FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Field, RA Stegelmeier, BL Colegate, SM Brown, AW Green, BT AF Field, Reuel A. Stegelmeier, Bryan L. Colegate, Steven M. Brown, Ammon W. Green, Benedict T. TI An in vitro comparison of the cytotoxic potential of selected dehydropyrrolizidine alkaloids and some N-oxides SO TOXICON LA English DT Article DE Pyrrolizidine-alkaloid; Pyrrolizidine-alkaloid-N-oxide; Comparative-cytotoxicity; Chicken-CRL-2118; Hepatocellular-carcinoma ID DNA CROSS-LINKING; PYRROLIZIDINE ALKALOIDS; CARCINOGENIC ACTIVITY; TOXICITY; RATS; CELLS; LASIOCARPINE; METABOLISM; OFFICINALE; HEPATOTOXICITY AB Plants producing dehydropyrrolizidine alkaloids (DHPAs) are found throughout the world and they are dangerous to human and animal health. Several DHPAs are carcinogenic but only riddelliine has been classified as a potential human carcinogen by the National Toxicology Program. As DHPA-related carcinogenicity is probably linked to cytotoxicity, a model of CRL-2118 chicken hepatocyte cytotoxicity was developed to compare equimolar DHPA exposures between 19 and 300 mu M. Alkaloid-related cytotoxicity was estimated using cytomorphology, cell viability reflected by mitochondria] function and cellular degeneration reflected by media lactate dehydrogenase activity. Lasiocarpine induced cytotoxicity and decreased cell viability in a concentration dependent manner at 24 h. At similar concentrations and exposures of 48 and 72 h, seneciphylline, senecionine, monocrotaline and riddelliine were cytotoxic. None of the DHPA-N-oxides were significantly cytotoxic at these concentrations. Using graphic analyses the median cytotoxic concentration (DHPA concentration that produced 1/2 the maximum response) were estimated. The estimated descending order of cytotoxicity was lasiocarpine, seneciphylline, senecionine, heliotrine, riddelliine, monocrotaline, riddelliine-N-oxide, lycopsamine, intermedine, lasiocarpine-N-oxide and senecionine-N-oxide. This comparison identifies DHPAs that were more cytotoxic than carcinogenic riddelliine. Additional studies to better characterize the carcinogenic potential of these alkaloids are essential to better determine the risk they each may pose for human and animal health. Published by Elsevier Ltd. C1 [Field, Reuel A.; Stegelmeier, Bryan L.; Colegate, Steven M.; Brown, Ammon W.; Green, Benedict T.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84332 USA. RP Stegelmeier, BL (reprint author), 1150 E 1400 N, Logan, UT 84341 USA. EM bryan.stegelmeier@ars.usda.gov FU USDA Agriculture Research Service [NP215- 2080-32630-012-00] FX The authors acknowledge the contribution of Isabelle McCollum, Edward Knoppel, and James A. Pfister for their technical assistance. We also thank Drs. Kerry Rood and Chad Clancey for their constructive manuscript review. This work was funded through the USDA Agriculture Research Service NP215- 2080-32630-012-00. NR 46 TC 3 Z9 3 U1 0 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD APR PY 2015 VL 97 BP 36 EP 45 DI 10.1016/j.toxicon.2015.02.001 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA CE4MB UT WOS:000351803600005 PM 25666399 ER PT J AU Zhang, YBP Zhou, YB Sang, BY Wan, XC Yang, YO Zhang, JL Welker, TL Liu, KS AF Zhang, Yao-bei-ping Zhou, Yi-bin Sang, Ba-yi Wan, Xiao-chun Yang, Yan-ou Zhang, Jian-li Welker, Thomas L. Liu, Keshun TI Effect of dietary Chinese tea on growth performance, disease resistance and muscle fatty acid profile of channel catfish (Ictalurus punctatus) SO AQUACULTURE INTERNATIONAL LA English DT Article DE Chinese tea; Channel catfish; Growth performance; Fatty acid profile; Lipid deposition; Disease resistance ID JUVENILE OLIVE FLOUNDER; GREEN TEA; BODY-COMPOSITION; PARALICHTHYS-OLIVACEUS; ONCORHYNCHUS-MYKISS; CAMELLIA-SINENSIS; LIPID OXIDATION; STRESS RECOVERY; INDUCED OBESITY; RAINBOW-TROUT AB Six types of Chinese tea were incorporated into diets at four different levels (0, 1, 2 and 4 %) in order to determine their effects on the growth performance, fatty acid composition of muscle lipids and resistance to Aeromonas hydrophila infection of Ictalurus punctatus (channel catfish) fingerlings. Each diet was fed to catfish in triplicate tanks for 8 weeks. Results show that a 1 % Dragon Well tea (DWT) and Maofeng tea were significantly better than other diets (including the control) in improving weight gain (WG) and feed conversion rate (FCR) of fish; while the 4 % DWT increased FCR (P < 0.01) but decreased WG (P < 0.05). In addition, 4 % DWT resulted in a significant decrease in muscle lipid content. Pu-erh tea in 2 and 4 % dietary addition decreased saturated fatty acid and increased monounsaturated fatty acid content in muscle. The hepatosomatic index decreased significantly in fish fed the 4 % DWT diet. The mortality (%) of catfish challenged with A. hydrophila decreased with increasing addition of tea to the diet, and the mortality in DWT and Huangya tea (HYT) was lower than control groups (P < 0.05). In conclusion, diets supplemented with a low dose (1 %) of Chinese tea increased growth performance and enhanced the fish fillet quality. Addition of DWT or HYT tea at 4 % in channel catfish diets can improve resistance against A. hydrophila, but growth performance is reduced. C1 [Zhang, Yao-bei-ping; Zhou, Yi-bin; Sang, Ba-yi; Wan, Xiao-chun; Yang, Yan-ou; Zhang, Jian-li] Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Peoples R China. [Zhang, Yao-bei-ping; Zhou, Yi-bin; Sang, Ba-yi; Wan, Xiao-chun; Yang, Yan-ou; Zhang, Jian-li] Anhui Agr Univ, Minist Agr, Hefei 230036, Peoples R China. [Zhang, Yao-bei-ping] XiaoShan Food & Drug Adm, Hangzhou 311200, Zhejiang, Peoples R China. [Welker, Thomas L.] ARS, Hagerman Fish Culture Expt Stn, USDA, Hagerman, ID 83332 USA. [Liu, Keshun] ARS, Natl Small Grains & Potato Germplasm Res Unit, USDA, Aberdeen, ID 83210 USA. RP Zhou, YB (reprint author), Anhui Agr Univ, Key Lab Tea Biochem & Biotechnol, Minist Educ, Hefei 230036, Peoples R China. EM beiping@126.com; zhouyibin@ahau.edu.cn; 790925844@qq.com; xcwan@ahau.edu.cn; yangyanou@ahau.edu.cn; 809041730@qq.com; thomas.welker@ars.usda.gov; Keshun.Liu@ars.usda.gov FU International Science and Technology Cooperation Programme of the Ministry of Science and Technology [2011DFG33280] FX This research was supported by the International Science and Technology Cooperation Programme of the Ministry of Science and Technology (Project Number: 2011DFG33280). NR 51 TC 0 Z9 0 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0967-6120 EI 1573-143X J9 AQUACULT INT JI Aquac. Int. PD APR PY 2015 VL 23 IS 2 BP 683 EP 698 DI 10.1007/s10499-014-9846-7 PG 16 WC Fisheries SC Fisheries GA CD6WX UT WOS:000351232200020 ER PT J AU Khankhum, S Valverde, RA Pastor-Corrales, MA Osorno, JM Sabanadzovic, S AF Khankhum, Surasak Valverde, Rodrigo A. Pastor-Corrales, Marcial A. Osorno, Juan M. Sabanadzovic, Sead TI Two endornaviruses show differential infection patterns between gene pools of Phaseolus vulgaris SO ARCHIVES OF VIROLOGY LA English DT Article DE Andes; Common bean; dsRNA; Mesoamerica; Persistent viruses ID DOUBLE-STRANDED-RNA; DOMESTICATION; SEQUENCE; VIRUSES AB We investigated the occurrence of two plant endornaviruses, Phaseolus vulgaris endornavirus 1 and Phaseolus vulgaris endornavirus 2, in breeding lines, cultivars, landraces, and wild genotypes of common bean (Phaseolus vulgaris) collected from the two centers of common bean domestication: Mesoamerica and the Andes. The two endornaviruses were detected in many genotypes of Mesoamerican origin but rarely in genotypes of Andean origin. The results suggest that these two endornaviruses were introduced into the Mesoamerican modern genotypes during common bean domestication and provide more evidence for the existence of two divergent gene pools of common bean. C1 [Khankhum, Surasak; Valverde, Rodrigo A.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Pastor-Corrales, Marcial A.] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Osorno, Juan M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Sabanadzovic, Sead] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. RP Valverde, RA (reprint author), Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, 302 Life Sci Bldg, Baton Rouge, LA 70803 USA. EM ravalve@lsu.edu OI Sabanadzovic, Sead/0000-0002-2995-2633 FU USDA National Institute of Food and Agriculture FX We wish to thank the USDA-ARS, National Plant Germplasm System, Western Regional Plant Introduction Station, Pullman, WA, and Donald Halseth, Dept. of Horticulture, Cornell University, NY, for providing some common bean cultivars, landraces, wild P. vulgaris, and other Phaseolus species. We also thank Andrea Hebert, Louisiana State University, for editing the manuscript. Partial support for this investigation was provided by the USDA National Institute of Food and Agriculture. NR 22 TC 4 Z9 5 U1 2 U2 8 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD APR PY 2015 VL 160 IS 4 BP 1131 EP 1137 DI 10.1007/s00705-015-2335-0 PG 7 WC Virology SC Virology GA CE0RW UT WOS:000351514100027 PM 25623050 ER PT J AU Wraight, SP Ramos, ME AF Wraight, S. P. Ramos, M. E. TI Delayed efficacy of Beauveria bassiana foliar spray applications against Colorado potato beetle: Impacts of number and timing of applications on larval and next-generation adult populations SO BIOLOGICAL CONTROL LA English DT Article DE Beauveria bassiana; Leptinotarsa decemlineata; Application methods; Application timing; Microbial biological control ID LEPTINOTARSA-DECEMLINEATA; ENTOMOPATHOGENIC FUNGI; BACILLUS-THURINGIENSIS; METARHIZIUM-ANISOPLIAE; BIOLOGICAL-CONTROL; BALSAMO VUILLEMIN; DORYPHORAE DIPTERA; UNITED-STATES; NEW-YORK; CHRYSOMELIDAE AB Spray programs comprising multiple or single foliar applications of the fungal pathogen Beauveria bassiana strain GHA (Bb-GHA) made during morning (AM) vs. evening (PM) hours were tested against Colorado potato beetle Leptinotarsa decemlineata (CPB) in small research plots of potatoes over multiple field seasons, and efficacy against larval and first-generation adult populations was evaluated. The pathogen (formulated as BotaniGard WP (R)) was applied at the rate of 2.5 x 10(13) conidia in a spray volume of 468-480 L per ha. Modes of efficacy were investigated by collecting pre-pupal larvae and holding them in soil cages in the field and laboratory. Results revealed that larval control achievable with multiple-spray programs was poor regardless of environmental moisture conditions, but that emerging adult populations were reduced by an average of 80% (range 58-93%) relative to control populations. Programs comprising a single application of Bb targeted against late-instar larvae reduced adult populations by an average of 60% (range 36-75%). Overall mean differences in efficacy of PM vs. AM applications were not significant in terms of larval control and defoliation damage. Emergence of next-generation adults was significantly lower in the PM vs. AM treatments, but differences were small in terms of percent control. Average yields from the Bb-GHA multiple-spray programs were 18% greater than those from the controls; the mean 5% increase resulting from the Bb-GHA single-spray programs was not significant. Observations indicated that moderate to high rates of inoculation and infection were achieved under a broad range of environmental conditions in the crop canopy, but larvae persisted until they entered the soil to pupate. Most deaths due to mycosis occurred among pre-pupal larvae in their pupation cells in the soil. These results, in combination with similar findings of delayed efficacy reported by other researchers, indicate that foliar applications of Bb could be a significant component of an IPM program aimed at suppressing CPB populations on large farms or over large areas. Published by Elsevier Inc. C1 [Wraight, S. P.; Ramos, M. E.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Wraight, SP (reprint author), Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, 538 Tower Rd, Ithaca, NY 14853 USA. EM steve.wraight@ars.usda.gov NR 52 TC 4 Z9 4 U1 3 U2 27 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2015 VL 83 BP 51 EP 67 DI 10.1016/j.biocontrol.2014.12.019 PG 17 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CE2VG UT WOS:000351677500008 ER PT J AU Berg, SH Hough-Goldstein, J Lake, EC D'Amico, V AF Berg, Scott H. Hough-Goldstein, Judith Lake, Ellen C. D'Amico, Vincent TI Mile-a-minute weed (Persicaria perfoliata) and weevil (Rhinoncomimus latipes) response to varying moisture and temperature conditions SO BIOLOGICAL CONTROL LA English DT Article DE Water stress; Drought; Herbivory; Persicaria perfoliata; Rhinoncomimus latipes ID BIOLOGICAL-CONTROL AGENT; PARTHENIUM-HYSTEROPHORUS ASTERACEAE; ZYGOGRAMMA-BICOLORATA COLEOPTERA; KOROTYAEV COLEOPTERA; PHENOTYPIC PLASTICITY; INSECT PERFORMANCE; HOST-SPECIFICITY; L. POLYGONALES; WATER-STRESS; PLANT AB The combined effects of herbivory and water stress on growth and reproduction of mile-a-minute weed (Persicaria perfoliata (L.) H. Gross) were investigated in greenhouse trials over two years, with well-watered or water-limited plants either exposed or not exposed to herbivory by the mile-a-minute weevil (Rhinoncomimus latipes Korotyaev). Moisture limitation and weevil herbivory significantly reduced the number of seeds produced by P. perfoliata, with the fewest seeds produced when both factors were present. Seed weight was reduced by moisture limitation and weevil herbivory the second year, and seed viability was reduced by herbivory both years. Plant biomass was lower both years under conditions of water limitation, with an additional effect of herbivory the second year. Well-watered plants the second year also produced substantially more weevils than water-limited plants by the end of the season. Results are consistent with field observations suggesting that years of high rainfall allow resurgence of P. perfoliata plant populations that were previously suppressed by R. latipes. An additional environmental chamber trial assessed the interaction between the weed and weevil at two different temperatures. Here, plant mortality occurred only at the higher temperature with weevil herbivory, suggesting that herbivory has a greater negative effect on P. perfoliata under warm conditions. Additional studies on temperature effects are needed for a more complete understanding of interactions between P. perfoliata and R. latipes under different abiotic conditions. (C) 2015 Elsevier Inc. All rights reserved. C1 [Berg, Scott H.; Hough-Goldstein, Judith] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. [Lake, Ellen C.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [D'Amico, Vincent] US Forest Serv, No Res Stn, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. RP Hough-Goldstein, J (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, 531 S Coll Ave, Newark, DE 19716 USA. EM jhough@udel.edu FU Forest Health Technology Enterprise Team, USDA Forest Service FX Thanks to the Philip Alampi Beneficial Insects Laboratory, Trenton, NJ, for providing weevils for these experiments, and to William Bartz for assistance in the greenhouse. Funding was supplied by the Forest Health Technology Enterprise Team, USDA Forest Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity employer and provider. NR 41 TC 2 Z9 2 U1 2 U2 11 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2015 VL 83 BP 68 EP 74 DI 10.1016/j.biocontrol.2015.01.001 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CE2VG UT WOS:000351677500009 ER PT J AU Stewart, JF Will, RE Robertson, KM Nelson, CD AF Stewart, John F. Will, Rodney E. Robertson, Kevin M. Nelson, C. Dana TI Frequent fire protects shortleaf pine (Pinus echinata) from introgression by loblolly pine (P-taeda) SO CONSERVATION GENETICS LA English DT Article DE Shortleaf pine; Introgression; Fire ecology; Ecological genetics; Silviculture ID HYBRIDS AB Across much of the globe, fire is a major disturbance agent of forest and grassland communities. The removal of fire from previously fire-maintained ecosystems, which has occurred in many areas, changes species composition, favoring later less fire tolerant species over fire-adapted ones. A recent measured increase in the rate of hybridization between the fire-adapted shortleaf pine (Pinus echinata) and less fire-adapted loblolly pine (P. taeda) suggests that introgression may be an emerging threat to shortleaf pine as a genetically distinct species. We used 25 microsatellite markers on seedlings and saplings to test how the use of frequent fire affects the survival of hybrids between the two species by contrasting species makeup and hybridity in regularly burned areas (every 2 years) to that in neighboring unburned areas, both with mixed canopies of loblolly pine, shortleaf pine, and hybrids. The results show that frequent prescribed fire selects against loblolly pine and hybrids, restoring the community to one dominated by shortleaf pine. These results are the first to indicate that frequent fire can resist introgression between two co-occurring native species and that fire exclusion as a land management policy may be having unrecognized deleterious effects on the genetic integrity of species previously isolated from one another based on fire tolerance. C1 [Stewart, John F.; Will, Rodney E.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. [Robertson, Kevin M.] Tall Timbers Res Stn & Land Conservancy, Tallahassee, FL USA. [Nelson, C. Dana] US Forest Serv, USDA, Southern Res Stn, Southern Inst Forest Genet, Saucier, MS USA. RP Will, RE (reprint author), Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. EM rodney.will@okstate.edu RI Will, Rodney/G-8111-2011 FU United States Department of Agriculture Forest Service [12-CA-11330126-014]; McIntire-Stennis project [OKL0 2665] FX We thank Professor Emeritus C. G. Tauer for initiating this line of research and igniting interest in the importance of shortleaf pine as a species at risk. We thank J. Bradley for assisting in sampling, as well as Professor S. Hallgren for providing laboratory space. This project was funded by the United States Department of Agriculture Forest Service Cooperative Agreement 12-CA-11330126-014 and McIntire-Stennis project OKL0 2665. NR 17 TC 5 Z9 5 U1 5 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD APR PY 2015 VL 16 IS 2 BP 491 EP 495 DI 10.1007/s10592-014-0669-x PG 5 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA CD7RA UT WOS:000351287400019 ER PT J AU Kobayashi, Y Peterson, BC Waldbieser, GC AF Kobayashi, Y. Peterson, B. C. Waldbieser, G. C. TI Relationship between expression of muscle-specific uncoupling protein 2 messenger RNA and genetic selection toward growth in channel catfish SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Muscle growth; Uncoupling protein 2; Channel catfish; Gene; Food intake ID ICTALURUS-PUNCTATUS; EVOLUTIONARY HISTORY; GROWING FAMILIES; FEED-EFFICIENCY; FOOD-INTAKE; METABOLISM; UCP2; LIVER; FISH; POLYMORPHISMS AB This study tested the hypothesis that increased growth in channel catfish is associated with expression of the genes that code for uncoupling proteins (UCP) 2 and 3, members of the mitochondrial channel proteins involved in nutrient sensing and metabolism. The specific objective was to contrast the levels of UCP2 messenger RNA (mRNA) in fast vs slow growing catfish as well as in fed vs fasted catfish. Two distinct UCP2 transcripts were identified and named UCP2a and UCP2b, respectively. Nucleotide and amino acid sequence of catfish UCP2s were highly similar to UCP2 and other UCPs from other fish and mammals (>75%). Expression of UCP2a mRNA was detectable at very low levels in various meta bolically active tissues, whereas the expression of UCP2b mRNA was readily detectable in the muscle and heart. In a 21-wk feeding study, fish that grew faster had a greater percent body fat at the end of the study (P < 0.01). Expression of UCP2b mRNA tended to be lower (P < 0.10) in fast growing fish in the middle of the study although levels were similar at the beginning and the end of the study. In the fed vs fasted study, expression of UCP2b mRNA in muscle was increased (P < 0.05) in fish assigned to 30 d of fasting. Our results suggest that, based on the nucleotide and amino acid sequence similarities and, tissue mRNA distribution, catfish UCP2b may be the analog to UCP3. Moreover, our results suggest selection toward growth and associated fat accumulation appears to be independent of muscle UCP2b mRNA expression and UCP2b-mediated mechanisms. (C) 2015 Elsevier Inc. All rights reserved. C1 [Kobayashi, Y.] Ft Hays State Univ, Dept Biol Sci, Hays, KS 67601 USA. [Peterson, B. C.; Waldbieser, G. C.] ARS, USDA, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS USA. RP Kobayashi, Y (reprint author), Ft Hays State Univ, Dept Biol Sci, Hays, KS 67601 USA. EM y_kobayashi@fhsu.edu NR 40 TC 0 Z9 0 U1 0 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD APR PY 2015 VL 51 BP 56 EP 64 DI 10.1016/j.domaniend.2014.11.003 PG 9 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA CE0FU UT WOS:000351480700006 PM 25528205 ER PT J AU Timm, AL Pierce, RB AF Timm, Anne L. Pierce, Rodney B. TI Vegetative substrates used by larval northern pike in Rainy and Kabetogama Lakes, Minnesota SO ECOLOGY OF FRESHWATER FISH LA English DT Article DE northern pike; water level; light trap; larval; aquatic vegetation ID ST-LAWRENCE-RIVER; ESOX-LUCIUS; COUNT DATA; MODELS; ABUNDANCE; MUSKELLUNGE; REGRESSION; ECOLOGY; ZEROS AB Our objective was to identify characteristics of aquatic vegetative communities used as larval northern pike nursery habitat in Rainy and Kabetogama lakes, glacial shield reservoirs in northern Minnesota. Quatrefoil light traps fished at night were used to sample larval northern pike in 11 potential nursery areas. Larval northern pike were most commonly sampled among floating-leaf burreed Sparganium fluctuans, common burreed Sparganium eurycarpum, sedges Carex spp., hybrid cattail Typhaxglauca and wild rice Zizania palustris. A negative binomial model of light-trap catches using the presence/absence data for 2012 from all 11 bays included water elevation and the presence of cattail as significant variables. Ultimately, the species of vegetation may not be as important as the physical quality or form of the vegetation in supplying feeding and hiding cover. The aquatic plant species and structural forms encountered by larval northern pike suggest they use nearly any vegetated cover available in early spring. Water-level regulations that change availability of aquatic vegetation are likely to influence recruitment of northern pike to larger sizes. C1 [Timm, Anne L.] US Forest Serv, USDA, No Res Stn, Baltimore, MD 21228 USA. [Pierce, Rodney B.] Minnesota DNR, Fisheries & Wildlife, Grand Rapids, MN USA. RP Timm, AL (reprint author), US Forest Serv, USDA, No Res Stn, 5523 Res Pk Dr,Suite 350, Baltimore, MD 21228 USA. EM altimm@fs.fed.us FU International Joint Commission FX We thank the International Joint Commission for funding this project. We are indebted to John Stanovick for help with the statistical modelling and to Voyageurs National Park and Minnesota DNR for help with logistics in the field. We thank Douglas A. Wilcox and Cindy Tomcko for their comments to improve this manuscript. We thank James Anderson, Meredith Bacon, Tom Burri, Justin Carney, Abbey Crosby, Patrick Ferguson, Sarah Ferguson, Larry Kallemeyn, Claire Kissane, Jaime LeDuc, Ryan Maki, Eric Olson, Kevin Peterson, Wes Peterson, Clare Pillsbury, Nick Schlesser, Geof Smith, Sarah Szymaniak, Craig Treat, Emily Vinge, Ben Vondra and Howard Weinberg for help with the field work. NR 37 TC 0 Z9 0 U1 8 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-6691 EI 1600-0633 J9 ECOL FRESHW FISH JI Ecol. Freshw. Fish PD APR PY 2015 VL 24 IS 2 BP 225 EP 233 DI 10.1111/eff.12137 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CD8MN UT WOS:000351349400006 ER PT J AU Liu, G Liu, PL Yang, MY Zhang, Q Warrington, DN AF Liu, Gang Liu, Puling Yang, Mingyi Zhang, Qiong Warrington, David N. TI Establishment of chronofunctions for dark loessial soil on the central Loess Plateau based on soil chronosequences SO ENVIRONMENTAL EARTH SCIENCES LA English DT Article DE C-14; Chronofunction; Soil genesis; Dark loessial soil; Migration ID MARINE TERRACES; MASS-BALANCE; EVOLUTION; CALIBRATION; MICHIGAN; HOLOCENE; RATES; CHINA; USA AB The process of soil development can be studied quantitatively by analyzing the chronofunctions of soil based on its physical and chemical characteristics, which provide a basis for establishing soil development models. In this paper, the physical and chemical characteristics as well as the C-14 age of Holocene dark loessial soil profiles found in Luochuan and Yanchang areas on the central Loess Plateau were analyzed to establish soil chronosequences. Then, linear functions, logarithmic functions, and third-order polynomials were used to fit the soil chronosequences to establish the soil's chronofunctions, which were verified both theoretically and empirically. In the two soil profiles, third-order polynomials could best fit the age of clay (< 0.002 mm), silt (0.002-0.02 mm), and sand (0.02-2 mm), and their trends reflected the characteristics of dark loessial soil layers. The changes of soil organic carbon and pH in relation to soil age could be fitted by logarithmic functions. Lastly, third-order polynomials could best fit changes in the soil's CaCO3 content and Fe/Zr, K/Zr, P/Zr, Na/Zr and Mg/Zr ratios with soil age, which represented the migration processes of CaCO3 and various elements in the soil. C1 [Liu, Gang] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing 100048, Peoples R China. [Liu, Gang; Liu, Puling; Yang, Mingyi; Zhang, Qiong; Warrington, David N.] Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. [Liu, Gang] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Liu, G (reprint author), Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, 26 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China. EM gliu@foxmail.com FU National Natural Science Foundation of China [41201270]; State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin (China Institute of Water Resources and Hydropower Research) [IWHR-SKL-201219] FX This research was jointly supported by the National Natural Science Foundation of China (No. 41201270) and Open Research Fund of State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin (China Institute of Water Resources and Hydropower Research) (Grant No. IWHR-SKL-201219). We thank the anonymous reviewers for their helpful comments. NR 35 TC 0 Z9 0 U1 2 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1866-6280 EI 1866-6299 J9 ENVIRON EARTH SCI JI Environ. Earth Sci. PD APR PY 2015 VL 73 IS 8 BP 4313 EP 4323 DI 10.1007/s12665-014-3715-6 PG 11 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA CD9XK UT WOS:000351453600029 ER PT J AU Roche, SE Garner, MG Sanson, RL Cook, C Birch, C Backer, JA Dube, C Patyk, KA Stevenson, MA Yu, ZD Rawdon, TG Gauntlett, F AF Roche, S. E. Garner, M. G. Sanson, R. L. Cook, C. Birch, C. Backer, J. A. Dube, C. Patyk, K. A. Stevenson, M. A. Yu, Z. D. Rawdon, T. G. Gauntlett, F. TI Evaluating vaccination strategies to control foot-and-mouth disease: a model comparison study SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Disease spread; foot-and-mouth disease; model comparison; modelling; relative validation; vaccination ID EMERGENCY VACCINATION; SIMULATION-MODEL; EPIDEMIC; SPREAD; AUSTRALIA; OUTBREAK AB Simulation models can offer valuable insights into the effectiveness of different control strategies and act as important decision support tools when comparing and evaluating outbreak scenarios and control strategies. An international modelling study was performed to compare a range of vaccination strategies in the control of foot-and-mouth disease (FMD). Modelling groups from five countries (Australia, New Zealand, USA, UK, The Netherlands) participated in the study. Vaccination is increasingly being recognized as a potentially important tool in the control of FMD, although there is considerable uncertainty as to how and when it should be used. We sought to compare model outputs and assess the effectiveness of different vaccination strategies in the control of FMD. Using a standardized outbreak scenario based on data from an FMD exercise in the UK in 2010, the study showed general agreement between respective models in terms of the effectiveness of vaccination. Under the scenario assumptions, all models demonstrated that vaccination with 'stamping-out' of infected premises led to a significant reduction in predicted epidemic size and duration compared to the 'stamping-out' strategy alone. For all models there were advantages in vaccinating cattle-only rather than all species, using 3-km vaccination rings immediately around infected premises, and starting vaccination earlier in the control programme. This study has shown that certain vaccination strategies are robust even to substantial differences in model configurations. This result should increase end-user confidence in conclusions drawn from model outputs. These results can be used to support and develop effective policies for FMD control. C1 [Roche, S. E.; Garner, M. G.] Dept Agr, Epidemiol & One Hlth Program, Canberra, ACT, Australia. [Garner, M. G.] AsureQuality Ltd, Palmerston North, New Zealand. [Cook, C.; Birch, C.; Gauntlett, F.] Anim Hlth & Vet Labs Agcy, Weybridge, Surrey, England. [Backer, J. A.] Wageningen UR, Cent Vet Inst, Lelystad, Netherlands. [Dube, C.] Canadian Food Inspect Agcy, Anim Hlth Risk Assessment Unit, Toronto, ON, Canada. [Patyk, K. A.] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Sci Technol & Anal Serv,Ctr Epidemiol & Anim Hlth, Denver, CO USA. [Stevenson, M. A.] Univ Melbourne, Fac Vet Sci, Parkville, Vic 3052, Australia. [Yu, Z. D.; Rawdon, T. G.] Minist Primary Ind, Invest & Diagnost Ctr & Response Directorate, Wellington, New Zealand. RP Roche, SE (reprint author), Dept Agr, Epidemiol & One Hlth Program, Canberra, ACT, Australia. EM sharon.roche@agriculture.gov.au RI APHA, Staff publications/E-6082-2010; Birch, Colin/C-7572-2011 OI Stevenson, Mark/0000-0003-1890-9784; Birch, Colin/0000-0002-4608-9093 NR 34 TC 7 Z9 7 U1 3 U2 20 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD APR PY 2015 VL 143 IS 6 BP 1256 EP 1275 DI 10.1017/S0950268814001927 PG 20 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CD9JW UT WOS:000351415600016 PM 25078780 ER PT J AU Liu, S Gao, P Wang, XZ Davis, AR Baloch, AM Luan, FS AF Liu, Shi Gao, Peng Wang, Xuezheng Davis, Angela R. Baloch, Abdul Majeed Luan, Feishi TI Mapping of quantitative trait loci for lycopene content and fruit traits in Citrullus lanatus SO EUPHYTICA LA English DT Article DE Watermelon; Lycopene; CAPS; QTLs ID CUCUMIS-MELO L.; RECOMBINANT INBRED LINES; LINKAGE MAP; FLESH COLOR; BETA-CAROTENE; WATERMELON LINES; RED WATERMELON; CANARY YELLOW; MARKERS; TOMATO AB Lycopene content is an important factor for determining watermelon fruit quality. However, the low DNA polymorphism among cultivated watermelon (Citrullus lanatus) has hindered the ability to establish high quality genetic maps and study the quantitative trait loci (QTL) controlling the lycopene content trait. In this study, we successfully constructed a genetic map of watermelon to determine lycopene content and other horticultural fruit traits using a F-2 population developed from a cross between the two lines of watermelon LSW-177 and Cream of Saskatchewan. The genetic map contained 16 linkage groups covering a total length of 2,039.5 cM, which included 37 SSRs (Simple Sequence Repeat) and 107 CAPSs (Cleaved Amplified Polymorphic Sequences), with all of the CAPS markers developed from high-throughput re-sequencing of data from this study. Three CAPS markers (WII04E07-33,WII04E07-37,WII04E07-40) caused the F-2 population to perfectly co-segregate for each F-2 population plants. We also obtained 12 QTLs for all of the traits measured. Only one QTL (LCYB4.1) was detected with a high value of trait variation (83.50 %) that related to lycopene content and mapped on Chromosome 4 between CAPS markers WII04E07-33 and WII04E07-40, which could nearly account for all of the differences in lycopene content between the two parental strains. In this study, we highlighted 2,458 CAPS loci that were suitable for primer design with a polymorphism of 48.9 %, which is approximately a 12-fold increase from previous studies. The present map and QTLs will facilitate future studies on determining lycopene content related genes and cloning watermelon genes, while also providing for useful markers for breeding for lycopene content. C1 [Liu, Shi; Gao, Peng; Wang, Xuezheng; Baloch, Abdul Majeed; Luan, Feishi] Northeast Agr Univ, Hort Coll, Harbin 150030, Heilongjiang Pr, Peoples R China. [Liu, Shi; Gao, Peng; Wang, Xuezheng; Baloch, Abdul Majeed; Luan, Feishi] Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Harbin 150030, Heilongjiang Pr, Peoples R China. [Davis, Angela R.] ARS, South Cent Agr Res Lab, USDA, Washington, DC USA. RP Luan, FS (reprint author), Northeast Agr Univ, Hort Coll, 59 Mucai St, Harbin 150030, Heilongjiang Pr, Peoples R China. EM luanfeishi@sina.com FU National Nature Science Foundation of China [31272186]; China Agriculture Research System [CARS-26-02] FX This research was supported by the National Nature Science Foundation of China (31272186). This work was also supported by the China Agriculture Research System (CARS-26-02). NR 53 TC 2 Z9 3 U1 2 U2 45 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD APR PY 2015 VL 202 IS 3 BP 411 EP 426 DI 10.1007/s10681-014-1308-9 PG 16 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CD7SG UT WOS:000351291100007 ER PT J AU Manor, ML Cleveland, BM Kenney, PB Yao, JB Leeds, T AF Manor, Meghan L. Cleveland, Beth M. Kenney, P. Brett Yao, Jianbo Leeds, Tim TI Differences in growth, fillet quality, and fatty acid metabolism-related gene expression between juvenile male and female rainbow trout SO FISH PHYSIOLOGY AND BIOCHEMISTRY LA English DT Article DE Salmonids; PPAR; mTOR; Fatty acids; Fillet quality ID PROLIFERATOR ACTIVATED RECEPTORS; ONCORHYNCHUS-MYKISS; SEXUAL-MATURATION; ADIPOSE-TISSUE; LIPID-METABOLISM; ENERGY STORES; FEEDING LEVEL; MUSCLE; LIVER; TOR AB Sexual maturation occurs at the expense of stored energy and nutrients, including lipids; however, little is known regarding sex effects on nutrient regulatory mechanisms in rainbow trout prior to maturity. Thirty-two, 14-month-old, male and female rainbow trout were sampled for growth, carcass yield, fillet composition, and gene expression of liver, white muscle, and visceral adipose tissue. Growth parameters, including gonadosomatic index, were not affected by sex. Females had higher percent separable muscle yield, but there were no sex effects on fillet proximate composition. Fillet shear force indicated females produce firmer fillets than males. Male livers had greater expression of three cofactors within the mTOR signaling pathway that act to inhibit TORC1 assembly; mo25, rictor, and pras40. Male liver also exhibited increased expression of beta-oxidation genes cpt1b and ehhadh. These findings are indicative of increased mitochondrial b-oxidation in male liver. Females exhibited increased expression of the mTOR cofactor raptor in white muscle and had higher expression levels of several genes within the fatty acid synthesis pathway, including gpat, srebp1, scd1, and cd36. Female muscle also had increased expression of beta-oxidation genes cpt1d and cpt2. Increased expression of both fatty acid synthesis and beta-oxidation genes suggests female muscle may have greater fatty acid turnover. Differences between sexes were primarily associated with variation of gene expression within the mTOR signaling pathway. Overall, data suggest there is differential regulation of gene expression in male and female rainbow trout tissues prior to the onset of sexual maturity that may lead to nutrient repartitioning during maturation. C1 [Manor, Meghan L.; Kenney, P. Brett; Yao, Jianbo] W Virginia Univ, Davis Coll Agr Forestry & Consumer Sci, Div Anim & Nutr Sci, Morgantown, WV 26505 USA. [Cleveland, Beth M.; Leeds, Tim] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Manor, ML (reprint author), W Virginia Univ, Davis Coll Agr Forestry & Consumer Sci, Div Anim & Nutr Sci, 1042 Agr Sci Bldg,POB 6108, Morgantown, WV 26505 USA. EM MeghanLManor@gmail.com FU USDA/CSREE S National Research Initiative Competitive Grants Program [2007-35205-17914]; USDA/ARS CRIS [1930-31000-010-000D]; USDA-ARS [58-1930-0-059]; West Virginia University [WVA00643] FX This study was funded by USDA/CSREE S#2007-35205-17914 National Research Initiative Competitive Grants Program and supported by USDA/ARS CRIS Project 1930-31000-010-000D and USDA-ARS Cooperative Agreement No. 58-1930-0-059. Additional funding was porvided by West Virginia University Hatch Grant No. WVA00643. It is published with the approval of the West Virginia University Director of the Agricultural Station as scientific Paper No. 3232. We would like to thank NCCCWA staff members Dave Payne for his technical assistance during sample collection, and Jim Everson, Josh Kretzer, Jenea McGowan, and Kyle Jenkins for their assistance with animal care. We would also like to thank West Virginia University staff Dr. Mohammed Salem, Dr. Hao Ma, Johnni-Ann Sims, and Aunchalee Aussanasuwannakul for their technical support of this study, Dr. Scott Snyder (Zeigler Bros., Inc., Gardners, PA) for providing the unpelleted mash, and Dr. Rick Barrows (USDA, ARS, Bozeman, MT) for manufacturing the labeled feed used for estimating feed intake. NR 44 TC 0 Z9 0 U1 4 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-1742 EI 1573-5168 J9 FISH PHYSIOL BIOCHEM JI Fish Physiol. Biochem. PD APR PY 2015 VL 41 IS 2 BP 533 EP 547 DI 10.1007/s10695-015-0027-z PG 15 WC Biochemistry & Molecular Biology; Fisheries; Physiology SC Biochemistry & Molecular Biology; Fisheries; Physiology GA CE0PP UT WOS:000351506900017 PM 25673423 ER PT J AU Shang, L Guo, WC Nelson, SO AF Shang, Liang Guo, Wenchuan Nelson, Stuart O. TI Apple Variety Identification Based on Dielectric Spectra and Chemometric Methods SO FOOD ANALYTICAL METHODS LA English DT Article DE Apple; Dielectric properties; Learning vector quantization network; Support vector machine; Extreme learning machine ID EXTREME LEARNING-MACHINE; SUCCESSIVE PROJECTIONS ALGORITHM; NEAR-INFRARED SPECTROSCOPY; SUPPORT VECTOR MACHINES; LVQ NEURAL-NETWORK; VARIABLE SELECTION; NIR SPECTROSCOPY; MULTIVARIATE-ANALYSIS; HYPERSPECTRAL IMAGES; MASS-SPECTROMETRY AB The dielectric properties of 160 apples of three varieties were obtained from 10 to 1,800 MHz. Based on the Kennard-Stone algorithm, 106 apples were selected for calibration set and the remaining 54 apples were used for validation set. Principal component analysis (PCA) and successive projections algorithm (SPA) were used to extract characteristic variables from original full dielectric spectra (FS). The learning vector quantization (LVQ) network, support vector machine (SVM), and extreme learning machine (ELM) modeling algorithms were applied to build models to identify the varieties of apples. Results showed that the first three principal components, and two dielectric constants and ten loss factors were selected as characteristic variables by PCA and SPA, respectively. SPA-ELM and PCA-ELM, whose total average accuracy reached 99.5 and 99.0 %, respectively, had good potential in identifying apple varieties. The study indicates that the dielectric spectra with chemometrics are promising for identifying apple varieties nondestructively and accurately. C1 [Shang, Liang; Guo, Wenchuan] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Nelson, Stuart O.] ARS, Russell Res Ctr, USDA, Athens, GA 30605 USA. RP Guo, WC (reprint author), Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM guowenchuan69@126.com FU National Natural Science Foundation of China [31171720] FX This research was supported by a grant from the National Natural Science Foundation of China (project no. 31171720). NR 51 TC 5 Z9 5 U1 6 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1936-9751 EI 1936-976X J9 FOOD ANAL METHOD JI Food Anal. Meth. PD APR PY 2015 VL 8 IS 4 BP 1042 EP 1052 DI 10.1007/s12161-014-9985-5 PG 11 WC Food Science & Technology SC Food Science & Technology GA CD7RR UT WOS:000351289400027 ER PT J AU Marion, DA Phillips, JD Yocum, C Mehlhope, SH AF Marion, Daniel A. Phillips, Jonathan D. Yocum, Chad Mehlhope, Stephanie H. TI Stream channel responses and soil loss at off-highway vehicle stream crossings in the Ouachita National Forest (vol 216, pg 40, 2014) SO GEOMORPHOLOGY LA English DT Correction C1 [Marion, Daniel A.; Yocum, Chad] US Forest Serv, USDA, Southern Res Stn, Hot Springs, AR 71902 USA. [Marion, Daniel A.; Phillips, Jonathan D.] Univ Kentucky, Dept Geog, Lexington, KY 40506 USA. [Mehlhope, Stephanie H.] Univ Kentucky, Dept Biosyst & Agr Engn, Lexington, KY 40506 USA. RP Marion, DA (reprint author), US Forest Serv, USDA, Southern Res Stn, POB 1270, Hot Springs, AR 71902 USA. EM dmarion@fs.fed.us OI Marion, Daniel/0000-0002-5871-8969 NR 1 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X EI 1872-695X J9 GEOMORPHOLOGY JI Geomorphology PD APR 1 PY 2015 VL 234 BP 108 EP 108 DI 10.1016/j.geomorph.2015.01.027 PG 1 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CE4IV UT WOS:000351795200009 ER PT J AU Johnson, AJ Shukle, RH Chen, MS Srivastava, S Subramanyam, S Schemerhorn, BJ Weintraub, PG Moniem, HEMA Flanders, KL Buntin, GD Williams, CE AF Johnson, A. J. Shukle, R. H. Chen, M. -S. Srivastava, S. Subramanyam, S. Schemerhorn, B. J. Weintraub, P. G. Moniem, H. E. M. Abdel Flanders, K. L. Buntin, G. D. Williams, C. E. TI Differential expression of candidate salivary effector proteins in field collections of Hessian fly, Mayetiola destructor SO INSECT MOLECULAR BIOLOGY LA English DT Article DE Mayetiola destructor; gall midges; secreted salivary effector proteins; wheat; plant-insect interactions ID GENE CONFERRING RESISTANCE; SOUTHEASTERN UNITED-STATES; DIPTERA-CECIDOMYIIDAE; WINTER-WHEAT; GALL MIDGES; PROBE LEVEL; VIRULENCE; LARVAE; POPULATIONS; GLANDS AB Evidence is emerging that some proteins secreted by gall-forming parasites of plants act as effectors responsible for systemic changes in the host plant, such as galling and nutrient tissue formation. A large number of secreted salivary gland proteins (SSGPs) that are the putative effectors responsible for the physiological changes elicited in susceptible seedling wheat by Hessian fly, Mayetiola destructor (Say), larvae have been documented. However, how the genes encoding these candidate effectors might respond under field conditions is unknown. The goal of this study was to use microarray analysis to investigate variation in SSGP transcript abundance amongst field collections from different geographical regions (southeastern USA, central USA, and the Middle East). Results revealed significant variation in SSGP transcript abundance amongst the field collections studied. The field collections separated into three distinct groups that corresponded to the wheat classes grown in the different geographical regions as well as to recently described Hessian fly populations. These data support previous reports correlating Hessian fly population structure with micropopulation differences owing to agro-ecosystem parameters such as cultivation of regionally adapted wheat varieties, deployment of resistance genes and variation in climatic conditions. C1 [Johnson, A. J.; Shukle, R. H.; Schemerhorn, B. J.; Williams, C. E.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Johnson, A. J.; Shukle, R. H.; Schemerhorn, B. J.; Moniem, H. E. M. Abdel] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Chen, M. -S.] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Chen, M. -S.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Srivastava, S.] Duke Univ, Dept Stat, Durham, NC USA. [Subramanyam, S.; Williams, C. E.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Weintraub, P. G.] Agr Res Org, Gilat Res Ctr, Dn Negev, Israel. [Moniem, H. E. M. Abdel] Suez Canal Univ, Fac Sci, Dept Zool, Ismailia, Egypt. [Flanders, K. L.] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Buntin, G. D.] Univ Georgia, Coll Agr Expt Stat, Dept Entomol, Georgia Stn, Griffin, GA USA. RP Shukle, RH (reprint author), Purdue Univ, USDA ARS, Dept Entomol, W Lafayette, IN 47907 USA. EM shukle@purdue.edu FU USDA-ARS CRIS [3602-22000-016-00D] FX This is a joint contribution of the USDA-ARS and Purdue University and was supported through USDA-ARS CRIS number 3602-22000-016-00D. Mention of a commercial or proprietary product does not constitute an endorsement by the USDA. NR 65 TC 1 Z9 1 U1 2 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD APR PY 2015 VL 24 IS 2 BP 191 EP 202 DI 10.1111/imb.12148 PG 12 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CD9DD UT WOS:000351396600005 PM 25528896 ER PT J AU Inglett, GE Chen, DJ Liu, SX AF Inglett, George E. Chen, Diejun Liu, Sean X. TI Pasting and rheological properties of quinoa-oat composites SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Elasticity/viscosity; functional food; oat bran ID GLUCAN-RICH HYDROCOLLOIDS; BETA-GLUCAN; FLOUR; BARLEY; PRODUCTS; BEHAVIOR; TEXTURE; SYSTEMS; STARCH; NUTRIM AB Quinoa (Chenopodium, quinoa) flour, known for its essential amino acids, was composited with oat products containing -glucan known for lowering blood cholesterol and preventing heart disease. Quinoa-oat composites were developed and evaluated for their pasting and rheological properties by a Rapid Visco Analyzer (RVA) and an advanced rheometer. All quinoa-oat composites showed increased pasting viscosities with increases in oat contents. The elastic modulus G and viscous modulus G for all quinoa-oat composites were higher than quinoa, suggesting that oat products increased the viscous properties of composites. Shear-thinning properties were observed for all the composites. Improved water-holding capacities (WHC) were found for the composites containing quinoa with nutrim or oat bran concentrate compared to quinoa alone. All the WHC of quinoa-oat composites were increased with the higher amounts of oat components. These quinoa-oat composites have improved nutritional value and texture qualities suitable for functional food applications. C1 [Inglett, George E.; Chen, Diejun; Liu, Sean X.] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Inglett, GE (reprint author), ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM George.Inglett@ars.usda.gov NR 40 TC 2 Z9 2 U1 5 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD APR PY 2015 VL 50 IS 4 BP 878 EP 884 DI 10.1111/ijfs.12722 PG 7 WC Food Science & Technology SC Food Science & Technology GA CD9ZD UT WOS:000351459700005 ER PT J AU Moore, KD Martin, RS Bradford, WJ Marchant, CC Jones, DS Wojcik, MD Pfeiffer, RL Prueger, JH Hatfield, JL AF Moore, Kori D. Martin, Randal S. Bradford, William J. Marchant, Christian C. Jones, Derek S. Wojcik, Michael D. Pfeiffer, Richard L. Prueger, John H. Hatfield, Jerry L. TI Derivation and Use of Simple Relationships between Aerodynamic and Optical Particle Measurements SO JOURNAL OF ENVIRONMENTAL ENGINEERING LA English DT Article DE Air pollution; Particles; Monitoring; Instrumentation; Calibration; Remote sensing ID SAN-JOAQUIN VALLEY; PARTICULATE MATTER; PM10 MASS; ENVIRONMENT; FRACTION; MINIVOL; PM2.5; LOGAN; SIZE; UTAH AB A simple relationship, referred to as a mass conversion factor (MCF), is presented to convert optically based particle measurements to mass concentration. It is calculated from filter-based samples and optical particle counter (OPC) data on a daily or sample period basis. The MCF allows for greater temporal and spatial mass concentration information than typical filter-based measurements. Results of MCF calculations from several field studies are summarized. Pairwise comparisons from a collocated study with multiple OPCs and mass samplers suggest the minimum variability of the MCF is 5-10%. The variability of the MCF within a sample period during a field study with distributed samplers averaged 17-21%. In addition, the precision of the Airmetrics MiniVol Portable Air Sampler for particulate matter (PM) was typically <10%. Comparisons with federal reference method (FRM) samplers showed that MiniVols yield PM2.5 concentrations essentially equivalent to FRMs with slightly greater deviations from the FRM for PM 10 under tested ambient conditions. (C) 2014 American Society of Civil Engineers. C1 [Moore, Kori D.; Bradford, William J.; Marchant, Christian C.; Jones, Derek S.; Wojcik, Michael D.] Utah State Univ Res Fdn, Space Dynam Lab, North Logan, UT 84341 USA. [Moore, Kori D.; Martin, Randal S.] Utah State Univ, Dept Civil & Environm Engn, Logan, UT 84322 USA. [Bradford, William J.; Jones, Derek S.] Campbell Sci, Logan, UT 84321 USA. [Marchant, Christian C.] Natl Geospatial Intelligence Agcy, Springfield, VA 22150 USA. [Pfeiffer, Richard L.; Prueger, John H.; Hatfield, Jerry L.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. RP Moore, KD (reprint author), Utah State Univ Res Fdn, Space Dynam Lab, 1695 North Res Pk Way, North Logan, UT 84341 USA. EM kori.moore@sdl.usu.edu FU USDA [58-3625-9-743]; State of Utah, Department of Environmental Quality FX The research reported in this paper is partially based upon work supported by the USDA, Cooperative Agreement No. 58-3625-9-743. Any opinions, findings, conclusions, or recommendations expressed in this paper are those of the writers and do not necessarily reflect the view of the USDA. The State of Utah, Department of Environmental Quality, also provided funding and equipment support for some of the data collection. The writers thank the teams involved in conducting the field work. Mention of a trademark, proprietary product, or vendor is for information purposes only and does not constitute an endorsement by the USDA, State of Utah, Utah State University, or Space Dynamics Laboratory. NR 30 TC 2 Z9 2 U1 5 U2 18 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9372 EI 1943-7870 J9 J ENVIRON ENG JI J. Environ. Eng.-ASCE PD APR PY 2015 VL 141 IS 4 AR 04014078 DI 10.1061/(ASCE)EE.1943-7870.0000893 PG 9 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CD9YJ UT WOS:000351456800002 ER PT J AU Diep, CS Foster, MJ McKyer, ELJ Goodson, P Guidry, JJ Liew, J AF Diep, Cassandra S. Foster, Margaret J. McKyer, E. Lisako J. Goodson, Patricia Guidry, Jeffrey J. Liew, Jeffrey TI What are Asian-American Youth Consuming? A Systematic Literature Review SO JOURNAL OF IMMIGRANT AND MINORITY HEALTH LA English DT Article DE Nutrition; Children; Asian American ID CALCIUM-RICH FOOD; ENVIRONMENTAL-INFLUENCES; PHYSICAL-ACTIVITY; RISK-FACTORS; ACCULTURATION; CHINESE; CHILDREN; BEHAVIORS; OBESITY; HEALTH AB Numerous studies have explored dietary practices among children, but there are limited studies on children of Asian background in the US. This review had three aims: (a) review literature regarding Asian-American youth's dietary behaviors, (b) critically evaluate the methodological quality of such research, and (c) provide recommendations for future nutrition-related research on Asian-American youth. The authors conducted a systematic literature review through MEDLINE (EBSCO), CINAHL Plus with Full Text (EBSCO), and Embase (Ovid); extracted descriptive data; and evaluated methodological quality. Thirteen articles were included. Major findings included: (a) frequent consumption of milk, fruit, meat, unenriched white rice, vegetables, and high-fat and high-sugar items among Asian-American children and (b) acculturation's influences on diet, resulting in Asian-American youth consuming diets characterized by both Asian and American foods. Findings from this review may inform education and promotion programs and services for Asian Americans in the US. C1 [Diep, Cassandra S.] Baylor Coll Med, Dept Family & Community Med, Houston, TX 77030 USA. [Diep, Cassandra S.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Foster, Margaret J.] Texas A&M Univ, Med Sci Lib, College Stn, TX USA. [McKyer, E. Lisako J.; Goodson, Patricia; Guidry, Jeffrey J.] Texas A&M Univ, Dept Hlth & Kinesiol, College Stn, TX USA. [Liew, Jeffrey] Texas A&M Univ, Dept Educ Psychol, College Stn, TX 77843 USA. RP Diep, CS (reprint author), Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. EM cdiep@bcm.edu OI Foster, Margaret/0000-0002-4453-7788 FU Primary Care Research Training Grant from National Research Service Award [T32 HP10031] FX This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. The contents of this manuscript are part of C.S.D.'s dissertation work. C.S.D. was supported fully by a Primary Care Research Training Grant from National Research Service Award (#T32 HP10031). We are grateful for valuable comments from Tom Baranowski and the journal reviewers. NR 50 TC 3 Z9 3 U1 1 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1557-1912 EI 1557-1920 J9 J IMMIGR MINOR HEALT JI J. Immigr. Minor. Health PD APR PY 2015 VL 17 IS 2 BP 591 EP 604 DI 10.1007/s10903-013-9905-6 PG 14 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CE0UW UT WOS:000351524000035 PM 23996643 ER PT J AU Yang, YY Toor, GS Williams, CF AF Yang, Yun-Ya Toor, Gurpal S. Williams, Clinton F. TI Pharmaceuticals and organochlorine pesticides in sediments of an urban river in Florida, USA SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Organochlorine pesticides; Pharmaceuticals; River sediments; Urbanizing watershed ID WASTE-WATER TREATMENT; TANDEM MASS-SPECTROMETRY; TAMPA-BAY; SURFACE WATERS; ANTHROPOGENIC MARKER; AQUATIC ENVIRONMENT; SEWAGE-TREATMENT; NATIONAL RECONNAISSANCE; MICROBIAL-DEGRADATION; ORGANIC-COMPOUNDS AB Sediments from a rural to urban gradient along the Alafia River in Florida, USA, were collected to determine the risk of environmental contamination with legacy (organochlorine pesticides (OCPs)) and new contaminants (pharmaceuticals). Bed sediments (0-10 cm) collected from rural and urban sub-basins of the Alafia River were analyzed for OCPs and pharmaceuticals using standard gas chromatography and liquid chromatography-mass spectrometry techniques. Three most frequently detected pharmaceuticals in sediments were carbamazepine (100 % of samples), trimethoprim (89 % of samples), and pseudoephedrine (63 % of samples). While acetaminophen, diphenhydramine, lidocaine, and nicotine were detected in < 30 % of samples. The detection of caffeine in all sediment samples suggests that domestic wastewater from wastewater treatment plants and/or septic systems may be a contributing source at all the sites. Among the OCPs, endosulfan I was most frequently detected (37 % of samples), followed by delta-hexachlorocyclohexane (15 % of samples), gamma-chlordane and endosulfan II (both in 11 % of samples), and dichlorodiphenyldichloroethylene and methoxychlor (both in 7 % of samples). The lower concentrations of OCPs (sum 0-16.1 ng g(-1)) than pharmaceuticals (sum 0.5-61.9 ng g(-1)) in sediments are probably due to the historic use of OCPs since these were banned for use in the USA in the 1970s, while pharmaceuticals are still used. The variability in detection and concentrations of legacy and new compounds in rural and urban stream sediments is likely due to the different magnitude of input sources, site characteristics, and chemical properties of individual compounds. Significant positive correlations between OCPs and sediment properties (organic matter, silt, and clay) suggest that sediments are a major sink of various contaminants in the Alafia River. We conclude that the concentrations of both pharmaceuticals and OCPs in sediments of this urban river are relatively lower than existing literature; however, these can still be of environmental concern to aquatic organisms. C1 [Yang, Yun-Ya; Toor, Gurpal S.] Univ Florida, Inst Food & Agr Sci, Gulf Coast Res & Educ Ctr, Soil & Water Qual Lab, Wimauma, FL 33598 USA. [Williams, Clinton F.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85239 USA. RP Toor, GS (reprint author), Univ Florida, Inst Food & Agr Sci, Gulf Coast Res & Educ Ctr, Soil & Water Qual Lab, 14625 CR 672, Wimauma, FL 33598 USA. EM gstoor@ufl.edu FU Institute of Food and Agricultural Sciences of University of Florida; National Integrated Water Quality Grant Program from the USDA National Institute of Food and Agriculture [2011-51130-31173] FX We thank former chemist, Butch Bradlay, for analysis of OCPs and MS student, Stefan Kalev, for his assistance in creating a GIS map of study sites. This project was supported by Institute of Food and Agricultural Sciences of University of Florida and National Integrated Water Quality Grant Program no. 2011-51130-31173 from the USDA National Institute of Food and Agriculture. NR 62 TC 9 Z9 11 U1 7 U2 73 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD APR PY 2015 VL 15 IS 4 BP 993 EP 1004 DI 10.1007/s11368-015-1077-7 PG 12 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA CE0SN UT WOS:000351516400024 ER PT J AU Ngo, HL AF Ngo, Helen L. TI Lewis Base Additives Improve the Zeolite Ferrierite-Catalyzed Synthesis of Isostearic Acids SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Ferrierite zeolite; Isostearic acid; Lubricants; Proton sponge; Triphenylamine triphenylphosphine; Triphenylphosphine oxide; Tri-p(tolyl)phosphine ID ISOMERIZATION; ISOMERS; PROTON AB Isostearic acids (IA) are highly utilized for industrial purposes especially in the area of biolubricants, such as cosmetics and slip additives for polyolefin and related copolymer films. This study was designed to develop a zeolitic catalysis process for efficient IA production through isomerization of fatty acids. The process utilized zeolite protonated Ferrierite with a small amount of base additive to neutralize (i.e., poison) the acidic sites on the external surfaces of the zeolite particles to prevent side reactions. Of the six base additives examined, the proton sponge combined with the zeolite protonated Ferrierite was found to be the most effective for this isomerization. With only 0.5 wt% proton sponge additive to 5.0 wt% Ferrierite, the dimers were successfully suppressed from 20.6 wt% yield to 2.42 wt% with an IA yield of 83.4 wt% and a 98 % conversion. C1 ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Ngo, HL (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM Helen.Ngo@ars.usda.gov FU NIFA-AFRI [2013-67021-21142] FX I thank Mr. Bun Hong-Lai and Mrs. Renee Latona for experimental assistance, Dr. Michael Haas for his critical reading of the manuscript, and Mr. Zekai Lin from the University of Chicago for determining the dimensions of the base additives. Finally, I would like to thank NIFA-AFRI (Grant award # 2013-67021-21142) for providing partial funding support. NR 21 TC 0 Z9 0 U1 2 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD APR PY 2015 VL 92 IS 4 BP 613 EP 619 DI 10.1007/s11746-015-2608-5 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CE1QQ UT WOS:000351587400015 ER PT J AU Mahadwar, G Chauhan, KR Bhagavathy, GV Murphy, C Smith, AD Bhagwat, AA AF Mahadwar, G. Chauhan, K. R. Bhagavathy, G. V. Murphy, C. Smith, A. D. Bhagwat, A. A. TI Swarm motility of Salmonella enterica serovar Typhimurium is inhibited by compounds from fruit peel extracts SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE agricutural waste; bacterial motility; flagella synthesis; food safety; food-borne pathogens ID FRESH-CUT PRODUCE; ESCHERICHIA-COLI; ANTI-FLHD(4)C(2) FACTOR; EXPRESSION; SAFETY; GENE; YDIV AB Controlling spread of human pathogens on fresh produce is a top priority for public health reasons. Isolation of compounds from agricultural waste that would control spread of human pathogens was explored using Salmonella enterica serovar Typhimurium as a model organism. In the environment, micro-organisms migrate as a community' especially when they move on moist surfaces. This type of motility is characterized as swarming motility. We examined extracts from agricultural waste such as soya bean husk, peels of orange, pineapple, avocado and pomegranate for antiswarming activity. Avocado and pineapple peels showed moderate (similar to 40%) inhibition of swarming motility while pomegranate peel extract had high antiswarming activity (similar to 85% inhibition) and was examined in further detail. Although the pomegranate peel extract was acidic, swarm-inhibitory activity was not due to low pH and the peel extract did not inhibit growth of Salmonella. Among the key swarm motility regulatory genes, class II (fliF, fliA, fliT and fliZ) and class III (fliC and fliM) regulators were downregulated upon exposure to pomegranate peel extract. Pomegranate peels offer great potential as a bioactive repellent for pathogenic micro-organisms on moist surfaces. Significance and Impact of the StudyControlling the spread of food-borne pathogens in moist environments is an important microbial food safety issue. Isolation of compounds from agricultural waste (such as fruit peels) that would control spread of human pathogens was explored using Salmonella enterica serovar Typhimurium as a model organism. Pomegranate peels offer great potential as a bioactive repellent for pathogenic micro-organisms. C1 [Mahadwar, G.; Bhagwat, A. A.] ARS, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Chauhan, K. R.; Bhagavathy, G. V.] ARS, Invas Insect Biocontrol & Behav Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Murphy, C.] ARS, Soybean Genom Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Smith, A. D.] ARS, Diet Genom & Immunol Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Bhagwat, AA (reprint author), ARS, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA,BARC E, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Arvind.bhagwat@ars.usda.gov RI Viswanathan Bhagavathy, Ganga/H-5249-2015 OI Viswanathan Bhagavathy, Ganga/0000-0002-7674-6787 NR 28 TC 1 Z9 1 U1 1 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD APR PY 2015 VL 60 IS 4 BP 334 EP 340 DI 10.1111/lam.12364 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CD9CP UT WOS:000351395000005 PM 25422036 ER PT J AU Schimel, D Keller, M AF Schimel, David Keller, Michael TI Big questions, big science: meeting the challenges of global ecology SO OECOLOGIA LA English DT Article DE Project management; Systems engineering; National Ecological Observatory Network (NEON) ID INCREASING CO2; DIVERSITY; BIOSPHERE; CLIMATE AB Ecologists are increasingly tackling questions that require significant infrastucture, large experiments, networks of observations, and complex data and computation. Key hypotheses in ecology increasingly require more investment, and larger data sets to be tested than can be collected by a single investigator's or s group of investigator's labs, sustained for longer than a typical grant. Large-scale projects are expensive, so their scientific return on the investment has to justify the opportunity cost-the science foregone because resources were expended on a large project rather than supporting a number of individual projects. In addition, their management must be accountable and efficient in the use of significant resources, requiring the use of formal systems engineering and project management to mitigate risk of failure. Mapping the scientific method into formal project management requires both scientists able to work in the context, and a project implementation team sensitive to the unique requirements of ecology. Sponsoring agencies, under pressure from external and internal forces, experience many pressures that push them towards counterproductive project management but a scientific community aware and experienced in large project science can mitigate these tendencies. For big ecology to result in great science, ecologists must become informed, aware and engaged in the advocacy and governance of large ecological projects. C1 [Schimel, David] CALTECH, Jet Prop Lab, Pasadena, CA 91101 USA. [Keller, Michael] US Forest Serv, USDA, Int Inst Trop Forestry, San Juan, PR 00926 USA. [Keller, Michael] EMBRAPA Satellite Monitoring, Sao Paulo, Brazil. RP Schimel, D (reprint author), CALTECH, Jet Prop Lab, Pasadena, CA 91101 USA. EM dschimel@jpl.nasa.gov; mkeller.co2@gmail.com RI Keller, Michael/A-8976-2012 OI Keller, Michael/0000-0002-0253-3359 FU National Aeronautics and Space Administration FX We offer these insights to our Israeli colleagues as they contemplate a national network, the occasion for this special issue and to the broader community. We dedicate this to the dedicated community of NEON staff members, advisory group members and alumni. The research carried out at the Jet Propulsion Laboratory, California Institute of Technology, was under a contract with the National Aeronautics and Space Administration. Copyright 2014 California Institute of Technology. We thank all the colleagues, too many to mention, through whose efforts over many years, many projects have been not only spectacular scientifically but personally rewarding for us. NR 25 TC 9 Z9 9 U1 5 U2 41 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2015 VL 177 IS 4 BP 925 EP 934 DI 10.1007/s00442-015-3236-3 PG 10 WC Ecology SC Environmental Sciences & Ecology GA CE0CD UT WOS:000351470100002 PM 25680334 ER PT J AU Smith, MD La Pierre, KJ Collins, SL Knapp, AK Gross, KL Barrett, JE Frey, SD Gough, L Miller, RJ Morris, JT Rustad, LE Yarie, J AF Smith, Melinda D. La Pierre, Kimberly J. Collins, Scott L. Knapp, Alan K. Gross, Katherine L. Barrett, John E. Frey, Serita D. Gough, Laura Miller, Robert J. Morris, James T. Rustad, Lindsey E. Yarie, John TI Global environmental change and the nature of aboveground net primary productivity responses: insights from long-term experiments SO OECOLOGIA LA English DT Article DE Aboveground productivity; Hierarchical response framework (HRF); Long-Term Ecological Research (LTER) Network; Nutrient addition; Precipitation manipulation ID ATMOSPHERIC CARBON-DIOXIDE; TERRESTRIAL ECOSYSTEMS; NITROGEN DEPOSITION; PLANT-COMMUNITIES; BIODIVERSITY LOSS; SPECIES RICHNESS; CLIMATE-CHANGE; ELEVATED CO2; PHOSPHORUS LIMITATION; FOREST ECOSYSTEMS AB Many global change drivers chronically alter resource availability in terrestrial ecosystems. Such resource alterations are known to affect aboveground net primary production (ANPP) in the short term; however, it is unknown if patterns of response change through time. We examined the magnitude, direction, and pattern of ANPP responses to a wide range of global change drivers by compiling 73 datasets from long-term (> 5 years) experiments that varied by ecosystem type, length of manipulation, and the type of manipulation. Chronic resource alterations resulted in a significant change in ANPP irrespective of ecosystem type, the length of the experiment, and the resource manipulated. However, the pattern of ecosystem response over time varied with ecosystem type and manipulation length. Continuous directional responses were the most common pattern observed in herbaceous-dominated ecosystems. Continuous directional responses also were frequently observed in longer-term experiments (> 11 years) and were, in some cases, accompanied by large shifts in community composition. In contrast, stepped responses were common in forests and other ecosystems (salt marshes and dry valleys) and with nutrient manipulations. Our results suggest that the response of ANPP to chronic resource manipulations can be quite variable; however, responses persist once they occur, as few transient responses were observed. Shifts in plant community composition over time could be important determinants of patterns of terrestrial ecosystem sensitivity, but comparative, long-term studies are required to understand how and why ecosystems differ in their sensitivity to chronic resource alterations. C1 [Smith, Melinda D.; Knapp, Alan K.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Smith, Melinda D.; Knapp, Alan K.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [La Pierre, Kimberly J.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Collins, Scott L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Gross, Katherine L.] Michigan State Univ, WK Kellogg Biol Stn, Hickory Corners, MI 49060 USA. [Gross, Katherine L.] Michigan State Univ, Dept Plant Biol, Hickory Corners, MI USA. [Barrett, John E.] Virginia Tech, Dept Biol Sci, Blacksburg, VA USA. [Frey, Serita D.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Gough, Laura] Univ Texas Arlington, Dept Biol, Arlington, TX 76019 USA. [Miller, Robert J.] Univ Calif Santa Barbara, Inst Marine Sci, Santa Barbara, CA 93106 USA. [Morris, James T.] Univ S Carolina, Belle W Baruch Inst Marine & Coastal Sci, Columbia, SC 29208 USA. [Rustad, Lindsey E.] US Forest Serv, USDA, Durham, NH USA. [Yarie, John] Univ Alaska Fairbanks, Dept Nat Resources Management, Fairbanks, AK USA. RP Smith, MD (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM melinda.smith@colostate.edu RI Barrett, John/D-5851-2016; Collins, Scott/P-7742-2014; Smith, Melinda/J-8987-2014; OI Barrett, John/0000-0002-7610-0505; Collins, Scott/0000-0002-0193-2892; La Pierre, Kimberly/0000-0001-7056-4547 FU LTER Network Office FX This synthesis effort was a product of an LTER Working Group entitled "Long-term experiments in the LTER Network: synthesis and hypothesis testing." We thank the participants of the working group for their contributions; S. McNulty, J. Boggs, P. Edwards, F. Wood, and R. Oren for providing data for this analysis; and the LTER Network Office for providing support. We thank four anonymous reviewers for providing comments on previous versions of the manuscript. NR 79 TC 8 Z9 8 U1 17 U2 133 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2015 VL 177 IS 4 BP 935 EP 947 DI 10.1007/s00442-015-3230-9 PG 13 WC Ecology SC Environmental Sciences & Ecology GA CE0CD UT WOS:000351470100003 PM 25663370 ER PT J AU Mowll, W Blumenthal, DM Cherwin, K Smith, A Symstad, AJ Vermeire, LT Collins, SL Smith, MD Knapp, AK AF Mowll, Whitney Blumenthal, Dana M. Cherwin, Karie Smith, Anine Symstad, Amy J. Vermeire, Lance T. Collins, Scott L. Smith, Melinda D. Knapp, Alan K. TI Climatic controls of aboveground net primary production in semi-arid grasslands along a latitudinal gradient portend low sensitivity to warming SO OECOLOGIA LA English DT Article DE Climate change; Precipitation; Temperature; Standardized precipitation evaporation index; Evapotranspiration ID RAIN-USE EFFICIENCY; SHORTGRASS STEPPE; TERRESTRIAL ECOSYSTEMS; TALLGRASS PRAIRIE; INTERANNUAL VARIABILITY; SPECIES RESPONSES; PATAGONIAN STEPPE; TEMPERATE STEPPE; SOIL-MOISTURE; CARBON FLUXES AB Although climate models forecast warmer temperatures with a high degree of certainty, precipitation is the primary driver of aboveground net primary production (ANPP) in most grasslands. Conversely, variations in temperature seldom are related to patterns of ANPP. Thus forecasting responses to warming is a challenge, and raises the question: how sensitive will grassland ANPP be to warming? We evaluated climate and multi-year ANPP data (67 years) from eight western US grasslands arrayed along mean annual temperature (MAT; similar to 7-14 A degrees C) and mean annual precipitation (MAP; similar to 250-500 mm) gradients. We used regression and analysis of covariance to assess relationships between ANPP and temperature, as well as precipitation (annual and growing season) to evaluate temperature sensitivity of ANPP. We also related ANPP to the standardized precipitation evaporation index (SPEI), which combines precipitation and evapotranspiration to better represent moisture available for plant growth. Regression models indicated that variation in growing season temperature was negatively related to total and graminoid ANPP, but precipitation was a stronger predictor than temperature. Growing season temperature was also a significant parameter in more complex models, but again precipitation was consistently a stronger predictor of ANPP. Surprisingly, neither annual nor growing season SPEI were as strongly related to ANPP as precipitation. We conclude that forecasted warming likely will affect ANPP in these grasslands, but that predicting temperature effects from natural climatic gradients is difficult. This is because, unlike precipitation, warming effects can be positive or negative and moderated by shifts in the C-3/C-4 ratios of plant communities. C1 [Mowll, Whitney; Cherwin, Karie; Smith, Anine; Smith, Melinda D.; Knapp, Alan K.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Mowll, Whitney; Cherwin, Karie; Smith, Anine; Smith, Melinda D.; Knapp, Alan K.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Blumenthal, Dana M.] ARS, USDA, Ft Collins, CO 80526 USA. [Symstad, Amy J.] US Geol Survey, Northern Prairie Wildlife Res Ctr, Hot Springs, SD 57747 USA. [Vermeire, Lance T.] USDA ARS Ft Keogh LARRL, Miles City, MT 59301 USA. [Collins, Scott L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. RP Knapp, AK (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM aknapp@colostate.edu RI Collins, Scott/P-7742-2014; Smith, Melinda/J-8987-2014 OI Collins, Scott/0000-0002-0193-2892; FU US Department of Agriculture-Agricultural Research Service Climate Change, Soils and Emissions Program; US National Science Foundation (NSF) [1021559]; NSF Macrosystems Biology Program FX We thank Dr Ann Hess, Colorado State University, for her significant assistance with the statistical analysis. We also thank the technicians and scientists at the SGS and the Sevilleta LTER sites for collecting, managing and sharing ANPP data, and the scientists associated with the Prairie Heating and CO2 Enrichment (PHACE) project in Cheyenne, Wyoming. PHACE was supported by the US Department of Agriculture-Agricultural Research Service Climate Change, Soils and Emissions Program and the US National Science Foundation (NSF; DEB no. 1021559). Additional research support for this analysis came from the NSF Macrosystems Biology Program. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US government. NR 68 TC 5 Z9 6 U1 9 U2 68 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2015 VL 177 IS 4 BP 959 EP 969 DI 10.1007/s00442-015-3232-7 PG 11 WC Ecology SC Environmental Sciences & Ecology GA CE0CD UT WOS:000351470100005 PM 25669452 ER PT J AU Calero-Bernal, R Verma, SK Oliveira, S Yang, Y Rosenthal, BM Dubey, JP AF Calero-Bernal, R. Verma, S. K. Oliveira, S. Yang, Y. Rosenthal, B. M. Dubey, J. P. TI In the United States, negligible rates of zoonotic sarcocystosis occur in feral swine that, by contrast, frequently harbour infections with Sarcocystis miescheriana, a related parasite contracted from canids SO PARASITOLOGY LA English DT Article DE Sarcocystis; feral pigs; Sus scrofa; PCR-RFLP; parasitic load; food safety; USA ID RIBOSOMAL-RNA GENE; DOMESTIC PIGS; PREVALENCE; SUIHOMINIS; SUICANIS; SARCOSPORIDIA AB Transmission of pathogens between domestic and wild life animals plays an important role in epidemiology. Feral pig populations are increasing and expanding in the USA, and may constitute a risk to non-biosecure domestic pig facilities by serving as reservoirs for pathogens. We surveyed, for Sarcocystis infection, the myocardium of 1006 feral pigs (Sus scrofa) trapped or hunted in 29 states during the Comprehensive Feral Swine Disease Surveillance Program of the USDA's Animal and Plant Health Inspection Service, Wildlife Services unit during 2012-2014. Sarcocysts were detected in histological sections of 25% (251/1006) of myocardium with an average parasitic load/intensity of infection of 303 sarcocysts/section (15x07 cm), and higher prevalence of myocarditis in severe infections. Microscopic examination of pepsin digests of 147 hearts revealed a higher prevalence of Sarcocystis bradyzoites (49%, 72/147) than when diagnosed by histology. A fragment of Sarcocystis 18S rRNA was amplified and digested with a restriction endonuclease, revealing a pattern consistent with Sarcocystis miescheriana in all 44 selected samples. Sequencing 31 of these 44 isolates confirmed their correspondence to S. miescheriana. Thus, S. miescheriana infection, but not the zoonotic parasite Sarcocystis suihominis, appears to be prevalent and widespread in feral pigs in the USA. C1 [Calero-Bernal, R.; Verma, S. K.; Oliveira, S.; Yang, Y.; Rosenthal, B. M.; Dubey, J. P.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov OI Rosenthal, Benjamin/0000-0002-0224-3773 FU Department of Employment and Innovation of the Regional Government of Extremadura (Spain) [PO12010]; European Social Fund; China Scholarship Council FX Rafael Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund, and Yurong Yang is a visiting scientist from Henan Agricultural University, Peoples Republic of China and funded by China Scholarship Council. NR 33 TC 3 Z9 3 U1 0 U2 2 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD APR PY 2015 VL 142 IS 4 BP 549 EP 556 DI 10.1017/S0031182014001553 PG 8 WC Parasitology SC Parasitology GA CD7LU UT WOS:000351272700004 PM 25363485 ER PT J AU Suresh, U Murugan, K Benelli, G Nicoletti, M Barnard, DR Panneerselvam, C Kumar, PM Subramaniam, J Dinesh, D Chandramohan, B AF Suresh, Udaiyan Murugan, Kadarkarai Benelli, Giovanni Nicoletti, Marcello Barnard, Donald R. Panneerselvam, Chellasamy Kumar, Palanisamy Mahesh Subramaniam, Jayapal Dinesh, Devakumar Chandramohan, Balamurugan TI Tackling the growing threat of dengue: Phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector Aedes aegypti (Diptera: Culicidae) SO PARASITOLOGY RESEARCH LA English DT Article DE Fourier transform infrared spectroscopy; Green synthesis; Mosquitocidal nanoparticles; Mosquito-borne diseases; Nanobiotechnologies; Phyllanthaceae; UV-vis spectroscopy; X-ray diffraction ID STEPHENSI LISTON DIPTERA; ANOPHELES-STEPHENSI; CULEX-QUINQUEFASCIATUS; LARVICIDAL ACTIVITY; LEAF EXTRACT; GOLD NANOTRIANGLES; GREEN SYNTHESIS; ESSENTIAL OILS; EUPHORBIACEAE; REPELLENT AB Mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. Dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. Recently, transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. Aedes aegypti (Diptera: Culicidae) is the primary vector of dengue. The use of synthetic insecticides to control Aedes mosquitoes lead to high operational costs and adverse nontarget effects. In this scenario, eco-friendly control tools are a priority. We proposed a novel method to synthesize silver nanoparticles using the aqueous leaf extract of Phyllanthus niruri, a cheap and nontoxic material. The UV-vis spectrum of the aqueous medium containing silver nanostructures showed a peak at 420 nm corresponding to the surface plasmon resonance band of nanoparticles. SEM analyses of the synthesized nanoparticles showed a mean size of 30-60 nm. EDX spectrum showed the chemical composition of the synthesized nanoparticles. XRD highlighted that the nanoparticles are crystalline in nature with face-centered cubic geometry. Fourier transform infrared spectroscopy (FTIR) of nanoparticles exhibited prominent peaks 3,327.63, 2,125.87, 1,637.89, 644.35, 597.41, and 554.63 cm(-1). In laboratory assays, the aqueous extract of P. niruri was toxic against larval instars (I-IV) and pupae of A. aegypti. LC50 was 158.24 ppm (I), 183.20 ppm (II), 210.53 ppm (III), 210.53 ppm (IV), and 358.08 ppm (pupae). P. niruri-synthesized nanoparticles were highly effective against A. aegypti, with LC50 of 3.90 ppm (I), 5.01 ppm (II), 6.2 ppm (III), 8.9 ppm (IV), and 13.04 ppm (pupae). In the field, the application of silver nanoparticles (10xLC(50)) lead to A. aegypti larval reduction of 47.6 %, 76.7% and 100 %, after 24, 48, and 72 h, while the P. niruri extract lead to 39.9 %, 69.2 % and 100 % of reduction, respectively. In adulticidal experiments, P. niruri extract and nanoparticles showed LC50 and LC90 of 174.14 and 6.68 ppm and 422.29 and 23.58 ppm, respectively. Overall, this study highlights that the possibility to employ P. niruri leaf extract and green-synthesized silver nanoparticles in mosquito control programs is concrete, since both are effective at lower doses if compared to synthetic products currently marketed, thus they could be an advantageous alternative to build newer and safer tools against dengue vectors. C1 [Suresh, Udaiyan; Murugan, Kadarkarai; Panneerselvam, Chellasamy; Kumar, Palanisamy Mahesh; Subramaniam, Jayapal; Dinesh, Devakumar; Chandramohan, Balamurugan] Bharathiar Univ, Sch Life Sci, Dept Zool, Div Entomol, Coimbatore 641046, Tamil Nadu, India. [Benelli, Giovanni] Univ Pisa, Dept Agr Food & Environm, Insect Behav Grp, I-56124 Pisa, Italy. [Nicoletti, Marcello] Univ Roma La Sapienza, Dept Environm Biol, I-00185 Rome, Italy. [Barnard, Donald R.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. RP Benelli, G (reprint author), Univ Pisa, Dept Agr Food & Environm, Insect Behav Grp, Via Borghetto 80, I-56124 Pisa, Italy. EM kmvvkg@gmail.com; g.benelli@sssup.it RI Benelli, Giovanni/A-9100-2013; OI Benelli, Giovanni/0000-0001-8971-6010 FU Department of Science and Technology (New Delhi, India) [DST/SB/EMEQ-335/2013]; Mis. 124 MODOLIVI Grant; Italian Ministry of Education, University and Research (MIUR) FX Kadarkarai Murugan is grateful to the Department of Science and Technology (New Delhi, India), Project No. DST/SB/EMEQ-335/2013, for providing financial support. Giovanni Benelli is supported by a Mis. 124 MODOLIVI Grant. Funds were also provided by the Italian Ministry of Education, University and Research (MIUR). Funds were also provided by the Italian Ministry of Education, University and Research (MIUR). Funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 49 Z9 52 U1 4 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD APR PY 2015 VL 114 IS 4 BP 1551 EP 1562 DI 10.1007/s00436-015-4339-9 PG 12 WC Parasitology SC Parasitology GA CE0UV UT WOS:000351523900030 PM 25669140 ER PT J AU Rosser, TG Griffin, MJ Quiniou, SMA Khoo, LH Greenway, TE Wise, DJ Pote, LM AF Rosser, Thomas G. Griffin, Matt J. Quiniou, Sylvie M. A. Khoo, Lester H. Greenway, Terrence E. Wise, David J. Pote, Linda M. TI Small subunit ribosomal RNA sequence links the myxospore stage of Henneguya mississippiensis n. sp from channel catfish Ictalurus punctatus to an actinospore released by the benthic oligochaete Dero digitata SO PARASITOLOGY RESEARCH LA English DT Article DE Actinospore; Catfish; Henneguya; Ictalurus punctatus; Myxospore; Myxozoa ID PROLIFERATIVE GILL DISEASE; LIFE-CYCLE; TUBIFEX-TUBIFEX; ALTERNATE HOSTS; THELOHANELLUS-HOVORKAI; MYXOBOLUS-CEREBRALIS; MYXOZOA MYXOSPOREA; CAUSATIVE AGENT; RDNA SEQUENCES; DNA-SEQUENCE AB There are more than 200 species of Henneguya described from fish. Of these, only three life cycles have been determined, identifying the actinospore and myxospore stages from their respective hosts. Two of these life cycles involve the channel catfish (Ictalurus punctatus) and the freshwater oligochaete Dero digitata. Herein, we molecularly confirm the life cycle of a previously undescribed Henneguya sp. by matching 18S ribosomal RNA (rRNA) gene sequence of the myxospore stage from channel catfish with the previously described actinospore stage (Aurantiactinomyxon mississippiensis) from D. digitata. Gill tissue from naturally infected channel catfish contained pseudocysts restricted to the apical end of the primary lamellae. Myxospores were morphologically consistent with Henneguya spp. from ictalurid fishes in North America. The spores measured 48.8+/-4.8 mu m (range=40.7-61.6 mu m) in total spore length. The lanceolate spore body was 17.1+/-1.0 mu m (14.4-19.3 mu m) in length and 5.0+/-0.3 mu m (4.5-5.5 mu m) in width. The two polar capsules were 6.2+/-0.4 mu m (5.8-7.0 mu m) long and 5.0+/-0.3 mu m (4.55.5 mu m) wide. The polar capsule contained eight to nine coils in the polar filament. The two caudal processes were of equal length, measuring 31.0+/-4.1 mu m (22.9-40.6 mu m). The 1980-bp 18S rRNA gene sequence obtained from two excised cysts shared 99.4 % similarity (100 % coverage) to the published sequence of A. mississippiensis, an actinospore previously described from D. digitata. The sequence similarity between the myxospore from channel catfish and actinospore from D. digitata suggests that they are conspecific, representing alternate life stages of Henneguya mississippiensis n. sp. C1 [Rosser, Thomas G.; Pote, Linda M.] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Griffin, Matt J.; Khoo, Lester H.] Mississippi State Univ, Coll Vet Med, Dept Pathobiol & Populat Med, Mississippi State, MS 39762 USA. [Griffin, Matt J.; Khoo, Lester H.; Greenway, Terrence E.; Wise, David J.] Mississippi State Univ, Delta Res & Extens Ctr, Aquat Res & Diagnost Lab, Thad Cochran Natl Warmwater Aquaculture Ctr, Washington Cty, MS 38776 USA. [Quiniou, Sylvie M. A.] ARS, Warmwater Aquaculture Res Unit, USDA, Stoneville, MS 38776 USA. [Greenway, Terrence E.; Wise, David J.] Mississippi State Univ, Mississippi Agr & Forestry Expt Stn, Mississippi State, MS 39762 USA. RP Pote, LM (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM lpote@cvm.msstate.edu FU Mississippi State University College of Veterinary Medicine; Mississippi Agricultural and Forestry Experiment Station; USDAARS Catfish Health Initiative [CRIS 6402-31320-002-02] FX This research was supported by the Mississippi State University College of Veterinary Medicine, the Mississippi Agricultural and Forestry Experiment Station, and the USDAARS Catfish Health Initiative (CRIS 6402-31320-002-02). This is MAFES publication number: J-12614. NR 55 TC 4 Z9 4 U1 1 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD APR PY 2015 VL 114 IS 4 BP 1595 EP 1602 DI 10.1007/s00436-015-4345-y PG 8 WC Parasitology SC Parasitology GA CE0UV UT WOS:000351523900033 PM 25716821 ER PT J AU Lee, KR Chen, GQ Kim, HU AF Lee, Kyeong-Ryeol Chen, Grace Q. Kim, Hyun Uk TI Current progress towards the metabolic engineering of plant seed oil for hydroxy fatty acids production SO PLANT CELL REPORTS LA English DT Review DE Hydroxy fatty acid; Industrial fatty acid; Castor; Lesquerella; Arabidopsis; Transgenic oilseed crop ID STABLE GENETIC-TRANSFORMATION; RICINUS-COMMUNIS L.; DIACYLGLYCEROL ACYLTRANSFERASE GENE; COENZYME-A SYNTHETASE; ACYL-COA; ARABIDOPSIS-THALIANA; LESQUERELLA-FENDLERI; TRIACYLGLYCEROL SYNTHESIS; TRANSGENIC ARABIDOPSIS; ENZYMATIC-SYNTHESIS AB Hydroxy fatty acids produced in plant seed oil are important industrial material. This review focuses on the use of metabolic engineering approaches for the production of hydroxy fatty acids in transgenic plants. Vegetable oil is not only edible but can also be used for industrial purposes. The industrial demand for vegetable oil will increase with the continued depletion of fossil fuels and ensuing environmental issues such as climate change, caused by increased carbon dioxide in the air. Some plants accumulate high levels of unusual fatty acids in their seeds, and these fatty acids (FAs) have properties that make them suitable for industrial applications. Hydroxy fatty acids (HFAs) are some of the most important of these industrial FAs. Castor oil is the conventional source of HFA. However, due to the presence of toxin ricin in its seeds, castor is not cultivated on a large scale. Lesquerella is another HFA accumulator and is currently being developed as a new crop for a safe source of HFAs. The mechanisms of HFA synthesis and accumulation have been extensively studied using castor genes and the model plant Arabidopsis. HFAs accumulated to 17 % in the seed oil of Arabidopsis expressing a FA hydroxylase gene from castor (RcFAH12), but its seed oil content and plant growth decreased. When RcFAH12 gene was coexpressed with additional castor gene(s) in Arabidopsis, similar to 30 % HFAs were accumulated and the seed oil content and plant growth was almost restored to the wild-type level. Further advancement of our understanding of pathways, genes and regulatory mechanisms underlying synthesis and accumulation of HFAs is essential to developing and implementing effective genetic approaches for enhancing HFA production in oilseeds. C1 [Lee, Kyeong-Ryeol; Kim, Hyun Uk] Natl Acad Agr Sci, Dept Agr Biotechnol, Rural Dev Adm, Jeonju 560500, South Korea. [Chen, Grace Q.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Kim, HU (reprint author), Natl Acad Agr Sci, Dept Agr Biotechnol, Rural Dev Adm, Jeonju 560500, South Korea. EM hukim64@korea.kr OI KIM, HYUN UK/0000-0002-4566-3057 FU Agricultural Research Project Program of the National Academy of Agricultural Science [PJ010075]; Rural Development Administration, Republic of Korea [PJ009484012014]; US Department of Agriculture-Agricultural Research Service-Current Research Information System Project [2030-21410-020-00D]; USDA Trust Fund Cooperative Agreement; RDA [58 0212 9 036F] FX This study was conducted with the support of the Agricultural Research Project Program (project no. PJ010075) of the National Academy of Agricultural Science, the "Next-Generation BioGreen 21 Program" (SSAC, project no. PJ009484012014) of the Rural Development Administration, Republic of Korea, the US Department of Agriculture-Agricultural Research Service-Current Research Information System Project 2030-21410-020-00D, and the USDA Trust Fund Cooperative Agreement with RDA (Agreement number: 58 0212 9 036F). The authors wish to thank Dr. Colleen McMahan for critical reading of the manuscript. USDA is an equal opportunity provider and employer. Mention of a specific product name by the United States Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 125 TC 4 Z9 5 U1 2 U2 41 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0721-7714 EI 1432-203X J9 PLANT CELL REP JI Plant Cell Reports PD APR PY 2015 VL 34 IS 4 SI SI BP 603 EP 615 DI 10.1007/s00299-015-1736-6 PG 13 WC Plant Sciences SC Plant Sciences GA CE2AA UT WOS:000351613300008 PM 25577331 ER PT J AU Pan, LQ Lu, RF Zhu, QB McGrath, JM Tu, K AF Pan, Leiqing Lu, Renfu Zhu, Qibing McGrath, J. Mitchell Tu, Kang TI Measurement of moisture, soluble solids, sucrose content and mechanical properties in sugar beet using portable visible and near-infrared spectroscopy SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Visible and near-infrared spectroscopy; Sugar beet; Moisture content; Soluble solids content; Sucrose content; Mechanical property ID REFLECTANCE SPECTROSCOPY; QUALITY ATTRIBUTES; FIRMNESS; FRUIT; SPECTRA; TEMPERATURE; PREDICTION; PEACHES; APPLES; WATER AB Visible and near-infrared spectroscopy, coupled with partial least squares regression, was used to predict the moisture, soluble solids and sucrose content and mechanical properties of sugar beet. Interactance spectra were acquired from both intact and sliced beets, using two portable spectrometers covering the spectral regions of 400-1100 nm and 900-1600 nm, respectively. Both visible and short-wave near-infrared (400-1100 nm) and near-infrared (900-1600 nm) spectrometers gave excellent predictions for the moisture, soluble solids and sucrose content of beet slices with the correlations (r(p)) of 0.89-0.95 and the standard errors of prediction (SEP) of 0.60-0.85. Lower prediction accuracies were obtained for intact beets, with the r(p) values of 0.75-0.85 and the SEPs of 0.88-1.23. However, the two spectrometers showed a poor ability of predicting the compressive mechanical properties (i.e., maximum force, area and the slope for the force/displacement curve) of both beet slices and intact beets. Using simple correlation analysis, we also identified wavelengths that had strong correlation with the measured compositions of sugar beets. The portable visible and near-infrared spectrometry is potentially useful for rapid assessment of the moisture, soluble solids and sucrose content of sugar beet at harvest and during postharvest handling and processing. (C) 2015 Elsevier B.V. All rights reserved. C1 [Pan, Leiqing; Tu, Kang] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China. [Lu, Renfu; McGrath, J. Mitchell] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. [Zhu, Qibing] Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, Wuxi 214122, Peoples R China. RP Pan, LQ (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. EM pan_leiqing@njau.edu.cn OI pan, leiqing/0000-0001-5587-3544 FU Chinese National Foundation of Natural Science [31101282, 71103086]; Special Fund for Agro-scientific Research in the Public Interest [201303088] FX The authors would like to thank Thomas Goodwill and R. Scott Shaw in the USDA Agricultural Research Service (ARS) Sugarbeet & Bean Research Unit at East Lansing, Michigan, for their technical support for the sucrose measurement by HPLC. The research was also supported by the Chinese National Foundation of Natural Science (31101282, 71103086) and Special Fund for Agro-scientific Research in the Public Interest (201303088). NR 37 TC 7 Z9 8 U1 7 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD APR PY 2015 VL 102 BP 42 EP 50 DI 10.1016/j.postharvbio.2015.02.005 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CE4FK UT WOS:000351786300006 ER PT J AU Sotos-Prieto, M Moreno-Franco, B Ordovas, JM Leon, M Casasnovas, JA Penalvo, JL AF Sotos-Prieto, Mercedes Moreno-Franco, Belen Ordovas, Jose M. Leon, Montse Casasnovas, Jose A. Penalvo, Jose L. TI Design and development of an instrument to measure overall lifestyle habits for epidemiological research: the Mediterranean Lifestyle (MEDLIFE) index SO PUBLIC HEALTH NUTRITION LA English DT Article DE Diet quality; Mediterranean diet; Diet index; Lifestyle ID PHYSICAL-ACTIVITY QUESTIONNAIRE; AMERICAN-HEART-ASSOCIATION; DIET-QUALITY SCORES; CARDIOVASCULAR-DISEASE; HEALTH-PROFESSIONALS; ADHERENCE; MORTALITY; WOMEN; RISK; POPULATION AB Objective: To design and develop a questionnaire that can account for an individual's adherence to a Mediterranean lifestyle including the assessment of diet and physical activity patterns, as well as social interaction. Design: The Mediterranean Lifestyle (MEDLIFE) index was created based on the current Spanish Mediterranean food guide pyramid. MEDLIFE is a twenty-eight-item derived index consisting of questions about food consumption (fifteen items), traditional Mediterranean dietary habits (seven items) and physical activity, rest and social interaction habits (six items). Linear regression models and Spearman rank correlation were fitted to assess content validity and internal consistency. Setting: A subset of participants in the Aragon Workers' Health Study cohort (Zaragoza, Spain) provided the data for development of MEDLIFE. Subjects: Participants (n 988) of the Aragon Workers' Health Study cohort in Spain. Results: Mean MEDLIFE score was 11.3 (SD 2.6; range: 0-28), and the quintile distribution of MEDLIFE score showed a significant association with each of the individual items as well as with specific nutrients and lifestyle indicators (intra-validity). We also quantified MEDLIFE correspondence with previously reported diet quality indices and found significant correlations (rho range: 0.44-0.53; P < 0.001) for the Alternate Healthy Eating Index, the Alternate Mediterranean Diet Index and Mediterranean Diet Adherence Screener. Conclusions: MEDLIFE is the first index to include an overall assessment of lifestyle habits. It is expected to be a more holistic tool to measure adherence to the Mediterranean lifestyle in epidemiological studies. C1 [Sotos-Prieto, Mercedes; Ordovas, Jose M.; Penalvo, Jose L.] Ctr Nacl Invest Cardiovasc, Dep Epidemiol Atherothrombosis & Imaging, Madrid 28029, Spain. [Sotos-Prieto, Mercedes] Int SHE Fdn, Barcelona 08037, Spain. [Moreno-Franco, Belen] Univ Zaragoza, Dept Med Psychiat & Dermatol, Zaragoza, Spain. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Leon, Montse; Casasnovas, Jose A.] Inst Aragones Ciencias Salud ICS, Cardiovasc Res Unit, Zaragoza, Spain. RP Sotos-Prieto, M (reprint author), Ctr Nacl Invest Cardiovasc, Dep Epidemiol Atherothrombosis & Imaging, Madrid 28029, Spain. EM msotos@externo.cnic.es FU Spanish National Research Fund R + D [PI11/00403] FX Financial support: This study was funded by the Spanish National Research Fund R + D, project PI11/00403, 2012-2015 (Polymorphism APOA2-265T > C in relation to dietary patterns and cardiovascular risk factors). The Spanish National Research Fund R + D had no role in the design, analysis or writing of this article. NR 33 TC 2 Z9 2 U1 1 U2 7 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD APR PY 2015 VL 18 IS 6 BP 959 EP 967 DI 10.1017/S1368980014001360 PG 9 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA CD7AZ UT WOS:000351243900002 PM 25025396 ER PT J AU Baranowski, T Beltran, A Chen, TA Thompson, D O'Connor, T Hughes, S Diep, C Baranowski, JC AF Baranowski, Tom Beltran, Alicia Chen, Tzu-An Thompson, Debbe O'Connor, Teresia Hughes, Sheryl Diep, Cassandra Baranowski, Janice C. TI Predicting use of ineffective vegetable parenting practices with the Model of Goal Directed Behavior SO PUBLIC HEALTH NUTRITION LA English DT Article DE Ineffective vegetable parenting practices; Model of goal-directed self-determined behaviour; Vegetable; Responsive parenting practices; Structure parenting practices; Controlling parenting practices ID HEALTHY LIFE-STYLES; SELF-DETERMINATION THEORY; EFFECTS FIREFIGHTER; CHILDRENS FRUIT; HABIT STRENGTH; INTERVENTIONS; EMOTIONS; PHLAME; CONSUMPTION; PREVENTION AB Objective: Increasing a parent's ability to influence a child's vegetable intake may require reducing the parent's use of ineffective vegetable parenting practices. The present study was designed to understand the psychosocial influences on ineffective vegetable parenting practices. Design: A cross-sectional web-based survey was conducted to model use of ineffective vegetable parenting practices using validated scales from a Model of Goal Directed Vegetable Parenting Practices. Setting: The dependent variable was a composite ineffective vegetable parenting practices index. The independent variables included validated subscales of intention, habit, perceived barriers, desire, competence, autonomy, relatedness, attitudes, norms, perceived behavioural control and anticipated emotions. Models were analysed using block regression with backward deletion. Subjects: Parents of 307 pre-school children (3-5 years old). Results: Variables significantly positively related to ineffective vegetable parenting practices in order of relationship strength included habit of controlling vegetable practices (standardized beta=0.349, P < 0.0001) and desire (standardized beta=0.117, P=0.025). Variables significantly negatively related to ineffective vegetable parenting practices in order of relationship strength included perceived behavioural control of negative parenting practices (standardized beta=-0.215, P < 0000), the habit of active child involvement in vegetable selection (standardized beta=-0.142, P=0.008), anticipated negative parent emotional response to child vegetable refusal (standardized beta=-0.133, P=0.009), autonomy (standardized beta=-0.118, P=0.014), attitude about negative effects of vegetables (standardized beta=-0.118, P=0.015) and descriptive norms (standardized beta=-0.103, P=0.032). The model accounted for 40.5% of the variance in use of ineffective vegetable parenting practices. Conclusions: The present study is the first report of psychometrically tested scales to predict use of ineffective vegetable parenting practices. Innovative intervention procedures will need to be designed and tested to reduce ineffective vegetable parenting practices. C1 [Baranowski, Tom; Beltran, Alicia; Chen, Tzu-An; Thompson, Debbe; O'Connor, Teresia; Hughes, Sheryl; Diep, Cassandra; Baranowski, Janice C.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. RP Baranowski, T (reprint author), USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institute of Child Health and Human Development [HD058175]; US Department of Agriculture, Agricultural Research Service [58-6250-6001] FX Financial support: This research was funded by the National Institute of Child Health and Human Development (grant number HD058175) and institutional support from the US Department of Agriculture, Agricultural Research Service (Cooperative Agreement no. 58-6250-6001). The funders had no role in the design, analysis or writing of this article. NR 38 TC 1 Z9 1 U1 2 U2 6 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD APR PY 2015 VL 18 IS 6 BP 1028 EP 1035 DI 10.1017/S1368980014001220 PG 8 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA CD7AZ UT WOS:000351243900010 PM 24946833 ER PT J AU Davis, GC Carlson, A AF Davis, George C. Carlson, Andrea TI The inverse relationship between food price and energy density: is it spurious? SO PUBLIC HEALTH NUTRITION LA English DT Article DE Energy density; Food cost; Price; Spurious ID LOW-INCOME WOMEN; ECONOMIC-ANALYSIS; DIET COSTS; UNITED-STATES; OBESITY; DISPARITIES; CALIFORNIA; VARIABLES AB Objective: An important debate in the literature is whether or not higher energy-dense foods are cheaper than less energy-dense foods. The present communication develops and applies an easy statistical test to determine if the relationship between food price and energy density is an artifact of how the data units are constructed (i.e. is it 'spurious' or 'real'?). Design: After matching data on 4430 different foods from the National Health and Nutrition Examination Survey with corresponding prices from the Center for Nutrition Policy and Promotion's Food Prices Database, we use a simple regression model to test if the relationship between food price and energy density is 'real' or 'spurious'. Setting: USA. Subjects: Total sample size is 4430 observations of consumed foods from 4578 participants from the non-institutionalized US adult population (aged 19 years and over). Results: Over all 4430 foods, the null hypothesis of a spurious inverse relationship between food price per energy density and energy density is not rejected. When the analysis is broken down by twenty-five food groups, there are only two cases where the inverse relationship is not spurious. In fact, the majority of non-spurious relationships between food price and energy density are positive, not negative. Conclusions: One of the main arguments put forth regarding the poor diet quality of low-income households is that high energy-dense food is cheaper than lower energy-dense food. We find almost no statistical support for higher energy-dense food being cheaper than low energy-dense food. While economics certainly plays a role in explaining low nutritional quality, more sophisticated economic arguments are required and discussed. C1 [Davis, George C.] Virginia Tech Univ, Dept Human Nutr Foods & Exercise, Blacksburg, VA 24061 USA. [Davis, George C.] Virginia Tech Univ, Agr & Appl Econ, Blacksburg, VA 24061 USA. [Carlson, Andrea] USDA, Econ Res Serv, Washington, DC USA. RP Davis, GC (reprint author), Virginia Tech Univ, Dept Human Nutr Foods & Exercise, 214 Hutcheson Hall, Blacksburg, VA 24061 USA. EM georgedavis@vt.edu NR 33 TC 5 Z9 5 U1 2 U2 10 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD APR PY 2015 VL 18 IS 6 BP 1091 EP 1097 DI 10.1017/S1368980014001098 PG 7 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA CD7AZ UT WOS:000351243900018 PM 24893111 ER PT J AU Wyborn, C Yung, L Murphy, D Williams, DR AF Wyborn, Carina Yung, Laurie Murphy, Daniel Williams, Daniel R. TI Situating adaptation: how governance challenges and perceptions of uncertainty influence adaptation in the Rocky Mountains SO REGIONAL ENVIRONMENTAL CHANGE LA English DT Article DE Adaptation; Adaptation pathways; Governance; Scale; Uncertainty; USA ID CLIMATE-CHANGE ADAPTATION; UNITED-STATES; ADAPTIVE CAPACITY; BARRIERS; FRAMEWORK; SCALE; VIEWS AB Adaptation is situated within multiple, interacting social, political, and economic forces. Adaptation pathways envision adaptation as a continual pathway of change and response embedded within this broader sociopolitical context. Pathways emphasize that current decisions are both informed by past actions and shape the landscape of future options. This research examines how adaptation actors in Grand County, Colorado perceive adaptation in the context of environmental change and uncertainty. Grand County residents drew on experiences of past change to suggest they had a high capacity to respond to future change, in particular a significant outbreak of mountain pine beetle. While residents and land managers characterized adaptation as gradual and incremental, they also recognized the ways that powerful cross-scale processes related to federal land management and water diversions challenged local adaptation. Further, Grand County residents identified multiple uncertainties in addition to those associated with climate projections, suggesting that addressing uncertainty extends beyond developing strategies robust across different climate scenarios. The challenges of uncertainty and cross-scale governance require more than increased adaptive capacity; they demand that we understand how local and extra-local structures shape the adaptation envelope that enables and constrains local decisions and implementation. Within this envelope, local actors pursue particular adaptation pathways and exercise agency to influence the structures shaping their options. Drawing on empirical insights, we argue that the concepts of pathways and envelopes together provide theoretical space for understanding the dynamic interplay between structure and agency in the context of adaptation. C1 [Wyborn, Carina; Yung, Laurie] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Murphy, Daniel] Univ Cincinnati, Dept Anthropol, Cincinnati, OH USA. [Williams, Daniel R.] United States Forest Serv, Rocky Mt Res Stn, Washington, DC USA. RP Wyborn, C (reprint author), Univ Montana, Coll Forestry & Conservat, Room 304,Main Hall, Missoula, MT 59812 USA. EM carina.wyborn@umontana.edu; laurie.yung@umontana.edu; murphdl@ucmail.uc.edu; drwilliams@fs.fed.us FU USDA Forest Service Rocky Mountain Research Station FX We wish to thank Grand County residents who participated in interviews and focus groups. We are grateful to the USDA Forest Service Rocky Mountain Research Station for financial support and for the contributions of Cory Cleveland, Solomon Dobrowski, Lisa Eby, Shannon McNeeley, and Erin Towler. NR 59 TC 7 Z9 7 U1 2 U2 25 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-3798 EI 1436-378X J9 REG ENVIRON CHANGE JI Reg. Envir. Chang. PD APR PY 2015 VL 15 IS 4 BP 669 EP 682 DI 10.1007/s10113-014-0663-3 PG 14 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA CD8VK UT WOS:000351374300009 ER PT J AU Anderson, RL AF Anderson, Randy L. TI Impact of corn residue on yield of cool-season crops SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE crop diversity; synergy; systems design; weed tolerance ID NO-TILL; ROTATION; AGROECOSYSTEMS; PRODUCTIVITY; TEMPERATURE; PLACEMENT; REMOVAL; FUTURE AB Synergy between dry pea and corn can reduce the density of corn needed for optimum yield. Lower crop density may accrue an additional benefit, as after-harvest residues of corn lying on the soil surface can reduce yield of crops planted the next year. This study evaluated impact of corn residue levels on growth and yield of three cool-season crops in no-till. Corn was grown at two densities, 52,000 and 73,000 plantsha-1, leading to after-harvest residue levels designated as low and high residue. Residue quantity on the soil surface differed by 21%. Controls were included for each residue level by burying residue with tillage. Spring wheat, dry pea and red clover were planted the following year. Grain yield of spring wheat and dry pea and forage yield of red clover were reduced 13-33% by residue on the soil surface. However, yield of cool-season crops were 10-18% higher in the low-residue treatment compared with high residue. Furthermore, yield loss because of weed interference in spring wheat and red clover was greater with high residue. Of the three crops, spring wheat was the least affected by corn residue on the soil surface. One contributing factor to lower yield with high residue was reduced crop seedling establishment. Producers may be able to reduce the negative impact of corn residue on following crops in no-till systems by using synergistic crop sequences in the rotation. C1 USDA ARS, Brookings, SD 57006 USA. RP Anderson, RL (reprint author), USDA ARS, Brookings, SD 57006 USA. EM randy.anderson@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 6 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD APR PY 2015 VL 30 IS 2 BP 184 EP 189 DI 10.1017/S174217051300032X PG 6 WC Agriculture, Multidisciplinary SC Agriculture GA CD3LT UT WOS:000350980700008 ER PT J AU Babiker, EM Gordon, TC Chao, S Newcomb, M Rouse, MN Jin, Y Wanyera, R Acevedo, M Brown-Guedira, G Williamson, S Bonman, JM AF Babiker, E. M. Gordon, T. C. Chao, S. Newcomb, M. Rouse, M. N. Jin, Y. Wanyera, R. Acevedo, M. Brown-Guedira, G. Williamson, S. Bonman, J. M. TI Mapping resistance to the Ug99 race group of the stem rust pathogen in a spring wheat landrace SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; 1ST REPORT; SOUTH-AFRICA; GENE SR22; VIRULENCE; SR31; GERMPLASM; LINES; TTKS; CULTIVARS AB A new gene for Ug99 resistance from wheat landrace PI 374670 was detected on the long arm of chromosome 7A. Wheat landrace PI 374670 has seedling and field resistance to stem rust caused by Puccinia graminis f. sp tritici Eriks. & E. Henn (Pgt) race TTKSK. To elucidate the inheritance of resistance, 216 BC1F2 families, 192 double haploid (DH) lines, and 185 recombinant inbred lines (RILs) were developed by crossing PI 374670 and the susceptible line LMPG-6. The parents and progeny were evaluated for seedling resistance to Pgt races TTKSK, MCCFC, and TPMKC. The DH lines were tested in field stem rust nurseries in Kenya and Ethiopia. The DH lines were genotyped with the 90K wheat iSelect SNP genotyping platform. Goodness-of-fit tests indicated that a single dominant gene in PI 374670 conditioned seedling resistance to the three Pgt races. The seedling resistance locus mapped to the long arm of chromosome 7A and this result was verified in the RIL population screened with the flanking SNP markers using KASP assays. In the same region, a major QTL for field resistance was detected in a 7.7 cM interval and explained 34-54 and 29-36 % of the variation in Kenya and Ethiopia, respectively. Results from tests with specific Pgt races and the csIH81 marker showed that the resistance was not due to Sr22. Thus, a new stem rust resistance gene or allele, either closely linked or allelic to Sr15, is responsible for the seedling and field resistance of PI 374670 to Ug99. C1 [Babiker, E. M.; Gordon, T. C.; Bonman, J. M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Chao, S.] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Newcomb, M.; Rouse, M. N.; Jin, Y.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Wanyera, R.] Kenya Agr & Livestock Res Org, Njoro 20107, Kenya. [Acevedo, M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Brown-Guedira, G.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27606 USA. [Williamson, S.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Babiker, EM (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM Ebrahiem.Babiker@ARS.USDA.GOV; Mike.Bonman@ARS.USDA.GOV RI Rouse, Matthew/G-5474-2011 FU USDA-ARS National Plant Disease Recovery System; USDA-ARS CRIS project [2050-21000-029-00D]; Durable Rust Resistance in Wheat (DRRW) project; Bill and Melinda Gates Foundation; United Kingdom Department for International development; National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX We are very grateful to Gebre Hiwot Abraha, Sam Stoxen, and Sam Gale for their technical assistance. This research was supported by the USDA-ARS National Plant Disease Recovery System, the USDA-ARS CRIS project 2050-21000-029-00D, and the Durable Rust Resistance in Wheat (DRRW) project managed by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International development. This work was supported in part by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. NR 33 TC 8 Z9 8 U1 0 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2015 VL 128 IS 4 BP 605 EP 612 DI 10.1007/s00122-015-2456-6 PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CD9RY UT WOS:000351437100004 PM 25599859 ER PT J AU Chen, SS Rouse, MN Zhang, WJ Jin, Y Akhunov, E Wei, YM Dubcovsky, J AF Chen, Shisheng Rouse, Matthew N. Zhang, Wenjun Jin, Yue Akhunov, Eduard Wei, Yuming Dubcovsky, Jorge TI Fine mapping and characterization of Sr21, a temperature-sensitive diploid wheat resistance gene effective against the Puccinia graminis f. sp tritici Ug99 race group SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID STEM RUST RESISTANCE; HEXAPLOID WHEAT; SOUTH-AFRICA; INFECTION TYPES; 1ST REPORT; GENOME; MONOCOCCUM; VIRULENCE; LINES; TTKS AB The diploid wheat stem rust resistance gene Sr21 confers temperature-sensitive resistance to isolates of the Ug99 group and maps to the middle of the long arm of chromosome 2A (m) . A race of Puccinia graminis f. sp. tritici, the causal pathogen of stem rust of wheat, known as Ug99, and its variants, are virulent to plants carrying stem rust resistance genes currently deployed in most wheat cultivars worldwide. Therefore, identification, mapping and deployment of effective resistance genes are critical to reduce this threat. Resistance gene Sr21 identified in diploid wheat T. monococcum can be effective against races from the Ug99 race group, but both susceptible and partial resistant reactions have been reported in previous studies. To clarify this conflicting information we screened four monogenic lines with Sr21 and four susceptible controls with 16 Pgt isolates including five isolates of the Ug99 race group under three different temperatures and three different photoperiods. We observed that, temperature influences the interaction between monogenic lines with Sr21 and Ug99 race group isolates, and may be one source of previous inconsistencies. This result indicates that, although Sr21 confers partial resistance against Ug99, its effectiveness can be modulated by environmental conditions and should not be deployed alone. Using two large diploid wheat-mapping populations (total 3,788 F-2 plants) we mapped Sr21 approximately 50 cM from the centromere on the long arm of chromosome 2A(m) within a 0.20 cM interval flanked by sequence-based markers FD527726 and EX594406. The closely linked markers identified in this study will be useful to reduce the T. monococcum segments introgressed into common wheat, accelerate Sr21 deployment in wheat breeding programs, and facilitate the map-based cloning of this gene. C1 [Chen, Shisheng; Zhang, Wenjun; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Chen, Shisheng; Wei, Yuming] Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. RP Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM jdubcovsky@ucdavis.edu RI Rouse, Matthew/G-5474-2011; Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU Borlaug Global Rust Initiative; National Research Initiative Competitive Grants from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) [2011-68002-30029, 2012-67013-19401]; USDA-Agricultural Research Service [3640-21220-021-00]; USDA National Plant Disease Recovery System; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation FX This project was supported by the Borlaug Global Rust Initiative, by the National Research Initiative Competitive Grants 2011-68002-30029 (Triticeae-CAP) and 2012-67013-19401 from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA), USDA-Agricultural Research Service appropriated project 3640-21220-021-00, USDA National Plant Disease Recovery System, and by support from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. We thank Mariana Padilla for excellent technical support. NR 42 TC 4 Z9 5 U1 1 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2015 VL 128 IS 4 BP 645 EP 656 DI 10.1007/s00122-015-2460-x PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CD9RY UT WOS:000351437100008 PM 25634104 ER PT J AU Mohammadi, M Blake, TK Budde, AD Chao, SM Hayes, PM Horsley, RD Obert, DE Ullrich, SE Smith, KP AF Mohammadi, Mohsen Blake, Thomas K. Budde, Allen D. Chao, Shiaoman Hayes, Patrick M. Horsley, Richard D. Obert, Donald E. Ullrich, Steven E. Smith, Kevin P. TI A genome-wide association study of malting quality across eight US barley breeding programs SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID POPULATION-STRUCTURE; LINKAGE DISEQUILIBRIUM; COMPLEX TRAITS; CULTIVATED BARLEY; QTL DETECTION; BREAD WHEAT; GERMPLASM; RESISTANCE; SELECTION; MAP AB We report malt quality QTLs relevant to breeding with greater precision than previous mapping studies. The distribution of favorable alleles suggests strategies for marker-assisted breeding and germplasm exchange. This study leverages the breeding data of 1,862 barley breeding lines evaluated in 97 field trials for genome-wide association study of malting quality traits in barley. The mapping panel consisted of six-row and two-row advanced breeding lines from eight breeding populations established at six public breeding programs across the United States. A total of 4,976 grain samples were subjected to micro-malting analysis and mapping of nine quality traits was conducted with 3,072 SNP markers distributed throughout the genome. Association mapping was performed for individual breeding populations and for combined six-row and two-row populations. Only 16 % of the QTL we report here had been detected in prior bi-parental mapping studies. Comparison of the analyses of the combined two-row and six-row panels identified only two QTL regions that were common to both. In total, 108 and 107 significant marker-trait associations were identified in all six-row and all two-row breeding programs, respectively. A total of 102 and 65 marker-trait associations were specific to individual six-row and two-row breeding programs, respectively indicating that most marker-trait associations were breeding population specific. Combining datasets from different breeding program resulted in both the loss of some QTL that were apparent in the analyses of individual programs and the discovery of new QTL not identified in individual programs. This suggests that simply increasing sample size by pooling samples with different breeding history does not necessarily increase the power to detect associations. The genetic architecture of malting quality and the distribution of favorable alleles suggest strategies for marker-assisted selection and germplasm exchange. C1 [Mohammadi, Mohsen; Smith, Kevin P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Blake, Thomas K.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Budde, Allen D.] USDA ARS, USA Cereal Crop Res Unit, Madison, WI 53726 USA. [Chao, Shiaoman] USDA ARS BRL, Fargo, ND 58102 USA. [Hayes, Patrick M.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Horsley, Richard D.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Obert, Donald E.] Limagrain Cereal Seeds, Ft Collins, CO 80525 USA. [Ullrich, Steven E.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Smith, KP (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. EM smith376@umn.edu FU United States Department of Agriculture-NIFA [2009-85606-05701, 2011-68002-30029]; American Malting Barley Association FX This research was supported by United States Department of Agriculture-NIFA Grant No. 2009-85606-05701, "Barley Coordinated Agricultural Project" and United States Department of Agriculture-NIFA Grant No. 2011-68002-30029, "Triticeae Coordinated Agricultural Project" and the American Malting Barley Association. NR 63 TC 6 Z9 6 U1 5 U2 51 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2015 VL 128 IS 4 BP 705 EP 721 DI 10.1007/s00122-015-2465-5 PG 17 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CD9RY UT WOS:000351437100013 PM 25666272 ER PT J AU Qi, LL Long, YM Jan, CC Ma, GJ Gulya, TJ AF Qi, L. L. Long, Y. M. Jan, C. C. Ma, G. J. Gulya, T. J. TI Pl(17) is a novel gene independent of known downy mildew resistance genes in the cultivated sunflower (Helianthus annuus L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MARKER-ASSISTED SELECTION; PCR-BASED MARKERS; PLASMOPARA-HALSTEDII; RUST RESISTANCE; FERTILITY RESTORATION; PL5/PL8 LOCUS; INHERITANCE; GENOME; RACES; EVOLUTION AB Pl(17) , a novel downy mildew resistance gene independent of known downy mildew resistance genes in sunflowers, was genetically mapped to linkage group 4 of the sunflower genome. Downy mildew (DM), caused by Plasmopara halstedii (Farl.). Berl. et de Toni, is one of the serious sunflower diseases in the world due to its high virulence and the variability of the pathogen. DM resistance in the USDA inbred line, HA 458, has been shown to be effective against all virulent races of P. halstedii currently identified in the USA. To determine the chromosomal location of this resistance, 186 F (2:3) families derived from a cross of HA 458 with HA 234 were phenotyped for their resistance to race 734 of P. halstedii. The segregation ratio of the population supported that the resistance was controlled by a single dominant gene, Pl (17) . Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) primers were used to identify molecular markers linked to Pl (17) . Bulked segregant analysis using 849 SSR markers located Pl (17) to linkage group (LG) 4, which is the first DM gene discovered in this linkage group. An F-2 population of 186 individuals was screened with polymorphic SSR and SNP primers from LG4. Two flanking markers, SNP SFW04052 and SSR ORS963, delineated Pl (17) in an interval of 3.0 cM. The markers linked to Pl (17) were validated in a BC3 population. A search for the physical location of flanking markers in sunflower genome sequences revealed that the Pl (17) region had a recombination frequency of 0.59 Mb/cM, which was a fourfold higher recombination rate relative to the genomic average. This region can be considered amenable to molecular manipulation for further map-based cloning of Pl(17) . C1 [Qi, L. L.; Jan, C. C.; Gulya, T. J.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. [Long, Y. M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Ma, G. J.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. RP Qi, LL (reprint author), ARS, USDA, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU National Sunflower Association [12-D02]; USDA-ARS CRIS Project [5442-21000-039-00D] FX The authors would like to thank Dr. Loren Rieseberg for providing access to the Sunflower Genome Data Repository. We also thank Drs. Brent Hulke and Zengcui Zhang for critical review of the manuscript, and Angelia Hogness for technical assistance. This project was supported by the National Sunflower Association Agreement 12-D02 and the USDA-ARS CRIS Project No. 5442-21000-039-00D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 72 TC 8 Z9 8 U1 0 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2015 VL 128 IS 4 BP 757 EP 767 DI 10.1007/s00122-015-2470-8 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CD9RY UT WOS:000351437100017 PM 25673143 ER PT J AU Coleman, TW Jones, MI Hoddle, MS Haavik, LJ Moser, JC Flint, ML Seybold, SJ AF Coleman, Tom W. Jones, Michael I. Hoddle, Mark S. Haavik, Laurel J. Moser, John C. Flint, Mary L. Seybold, Steven J. TI Pyemotes tritici (Acari: Pyemotidae): a parasitoid of Agrilus auroguttatus and Agrilus coxalis (Coleoptera: Buprestidae) in the southwestern United States of America and southern Mexico SO CANADIAN ENTOMOLOGIST LA English DT Article ID GOLDSPOTTED OAK BORER; STRAW ITCH MITE; CALIFORNIA; WATERHOUSE; WOODLANDS AB The straw itch mite, Pyemotes tritici Lagreze-Fossat and Montane (Acari: Pyemotidae), was discovered parasitising the goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), an invasive exotic species to California, United States of America, and the Mexican goldspotted oak borer, Agrilus coxalis Waterhouse (Coleoptera: Buprestidae), during surveys for natural enemies for a classical biological control programme for A. auroguttatus. Pyemotes tritici caused low levels of mortality to each species of flatheaded borer, but it will likely not be a good candidate for a biological control programme because it is a generalist parasitoid with deleterious human health effects. C1 [Coleman, Tom W.] US Forest Serv, USDA, Forest Hlth Protect, San Bernardino, CA 92408 USA. [Jones, Michael I.; Haavik, Laurel J.; Flint, Mary L.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Jones, Michael I.] SUNY Syracuse, Dept Environm & Forest Biol, Syracuse, NY 13210 USA. [Hoddle, Mark S.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Haavik, Laurel J.] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Moser, John C.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Chem Ecol Forest Insects, Davis, CA 95616 USA. RP Coleman, TW (reprint author), US Forest Serv, USDA, Forest Hlth Protect, 602S Tippecanoe Ave, San Bernardino, CA 92408 USA. EM twcoleman@fs.fed.us FU United States Department of Agriculture (USDA) Forest Service, Forest Health Protection, Region 5; USDA Forest Service Pacific Southwest Research Station Invasive Species Program and Southern Research Station; USDA Forest Service, Forest Health Protection-International Activities Team; University of California, Riverside and Davis FX The authors would like to thank United States Department of Agriculture (USDA) Forest Service, Forest Health Protection, Region 5; USDA Forest Service Pacific Southwest Research Station Invasive Species Program and Southern Research Station; USDA Forest Service, Forest Health Protection-International Activities Team; and the University of California, Riverside and Davis for funding this work. NR 23 TC 0 Z9 0 U1 1 U2 16 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD APR PY 2015 VL 147 IS 2 BP 244 EP 248 DI 10.4039/tce.2014.38 PG 5 WC Entomology SC Entomology GA CD2AF UT WOS:000350875200010 ER PT J AU Korntner, P Hosoya, T Dietz, T Eibinger, K Reiter, H Spitzbart, M Roeder, T Borgards, A Kreiner, W Mahler, AK Winter, H Groiss, Y French, AD Henniges, U Potthast, A Rosenau, T AF Korntner, Philipp Hosoya, Takashi Dietz, Thomas Eibinger, Klaus Reiter, Heidemarie Spitzbart, Martin Roeder, Thomas Borgards, Andrea Kreiner, Wolfgang Mahler, Arnulf Kai Winter, Heribert Groiss, Yvonne French, Alfred D. Henniges, Ute Potthast, Antje Rosenau, Thomas TI Chromophores in lignin-free cellulosic materials belong to three compound classes. Chromophores in cellulosics, XII SO CELLULOSE LA English DT Article DE Cellulose; Chromophores; Aging; Bleaching; Brightness; Brightness reversion ID N-OXIDE LYOCELL; AROMATIC-COMPOUNDS; AQUEOUS-SOLUTION; RESIDUAL CHROMOPHORES; CARBONYL GROUPS; BACTERIAL CELLULOSE; AGED CELLULOSICS; KEY CHROMOPHORE; D-GLUCOSE; IDENTIFICATION AB The chromophore release and identification method isolates well-defined chromophoric substances from different cellulosic matrices, such as highly bleached pulps, cotton linters, bacterial cellulose, viscose or lyocell fibers, and cellulose acetates. The chromophores are present only in extremely low (ppm to ppb) concentrations. The concept of primary and secondary chromophores is introduced, with primary chromophores arising only from the polysaccharides inherent to cellulosic materials. Secondary chromophores also include atoms from the chemicals used to process the cellulose. Most primary chromophores belong to one of three compound classes: hydroxy-[1,4]-benzoquinones, hydroxy-[1,4]-naphthoquinones, and hydroxyacetophenones. Among them, three individual compounds dominate: 2,5-dihydroxy-[1,4]-benzoquinone, 5,8-hydroxy-[1,4]-naphthoquinone, and 2,5-dihydroxyacetophenones, amounting to more than 80 % of the total isolated chromophores in most cases. In lignin-free cellulosics, these three compounds can thus be regarded as key chromophores. The prevalence of these molecules is due to both exceptionally strong resonance stabilization, as reflected in delocalized double bonds, and their ready reformation from carbohydrate degradation products by recondensation reactions. The findings that (a) most chromophores in lignin-free cellulosic materials belong to only three compound classes and that (b) three chromophore compounds make up the bulk of the chromophore mixtures are foundational for future bleaching research: Based on this knowledge, specific searches for optimized bleaching conditions can now concentrate on these compounds and still cover the vast majority of chromophores. C1 [Korntner, Philipp; Hosoya, Takashi; Henniges, Ute; Potthast, Antje; Rosenau, Thomas] Univ Nat Resources & Life Sci Vienna BOKU, Div Chem Renewables, Dept Chem, A-1190 Vienna, Austria. [Dietz, Thomas] Evon Degussa, D-63457 Hanau, Germany. [Eibinger, Klaus] Zellstoff Pols AG, A-8761 Pols, Austria. [Reiter, Heidemarie; Spitzbart, Martin] Mondi Uncoated Fine & Kraft Paper GmbH, A-1030 Vienna, Austria. [Roeder, Thomas; Borgards, Andrea] Lenzing AG, A-4860 Lenzing, Austria. [Kreiner, Wolfgang; Mahler, Arnulf Kai; Winter, Heribert] SAPPI Papier Holding GmbH, A-8101 Gratkorn, Austria. [Groiss, Yvonne] Austropapier, A-1060 Vienna, Austria. [French, Alfred D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Rosenau, T (reprint author), Univ Nat Resources & Life Sci Vienna BOKU, Div Chem Renewables, Dept Chem, Muthgasse 18, A-1190 Vienna, Austria. EM thomas.rosenau@boku.ac.at FU Austrian Christian-Doppler-Society through the "Christian Doppler Laboratory for Advanced Cellulose Chemistry and Analytics''; Austria Research Promotion Agency (FFG) FX We would like to thank the Austrian Christian-Doppler-Society for financial support through the "Christian Doppler Laboratory for Advanced Cellulose Chemistry and Analytics'' and the Austria Research Promotion Agency (FFG) and the industrial partners for financial support through the research project "Chromophores-elucidation of yellowing pathways in cellulosic structures". NR 30 TC 3 Z9 3 U1 2 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2015 VL 22 IS 2 BP 1053 EP 1062 DI 10.1007/s10570-015-0566-6 PG 10 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA CD2AQ UT WOS:000350876300009 ER PT J AU Qing, Y Sabo, R Wu, YQ Zhu, JY Cai, ZY AF Qing, Yan Sabo, Ronald Wu, Yiqiang Zhu, J. Y. Cai, Zhiyong TI Self-assembled optically transparent cellulose nanofibril films: effect of nanofibril morphology and drying procedure SO CELLULOSE LA English DT Article DE Cellulose nanofibrils; Self-assembled films; Filtration; Freeze-drying; Casting; Mechanical and optical properties ID MICROFIBRILLATED CELLULOSE; POLYMER NANOCOMPOSITES; NANOPAPER STRUCTURES; POLYVINYL-ALCOHOL; WOOD-CELLULOSE; TEMPLATES; FIBRILS; AEROGELS AB Cellulose nanofibril (CNF) films currently provide great opportunity in many applications with advantages of excellent mechanical strength, high light transmittance, and good barrier properties. However, processes for preparing CNFs are typically tedious and vary, along with their properties. Here, five preparation methods using various combinations of filtration, freeze-drying, and casting are applied to produce CNF films, and their major properties are compared. Three different types of CNFs having a range of fiber diameter and aspect ratio were examined using each of these five preparation methods. Because of limited hydrogen bonds and nanofibril arrangement, the freeze-dried CNF films displayed reduced mechanical strength and light transmittance compared to the other methods, although freeze-drying was relatively fast. Some effects of film production methods on measured crystallinity were also observed with freeze-dried samples having lower crystallinity than films similarly produced by filtration and drying. Free-standing CNF films produced by casting at room temperature required long times and mold growth was sometimes observed, but cast films made from 2,2,6,6-tetramethylpiperidine-1-oxyl radical-oxidized CNFs had the highest light transmittance of any samples. Filtration of CNF suspensions followed by air- or oven-drying produced films with minimal defects, high mechanical strength, and good light transmittance with relatively little effort. Therefore, this filtration procedure is recommended for producing CNF films. C1 [Qing, Yan; Wu, Yiqiang] Cent South Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China. [Qing, Yan; Sabo, Ronald; Zhu, J. Y.; Cai, Zhiyong] USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Sabo, R (reprint author), USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM rsabo@fs.fed.us; zcai@fs.fed.us FU Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture the Forestry, Industry Research Special Funds for Public Welfare Project of China [2011-67009-20056, 201404604]; Hunan Provincial Innovation Foundation for Postgraduate from China [CX2014A013] FX This work was financially supported by Agriculture and Food Research Initiative Grant (No. 2011-67009-20056) from the USDA National Institute of Food and Agriculture the Forestry, Industry Research Special Funds for Public Welfare Project of China (201404604), and Hunan Provincial Innovation Foundation for Postgraduate (CX2014A013) from China. The authors gratefully acknowledge Rick Reiner of the Forest Products Laboratory for preparing and supplying TEMPO-oxidized cellulose nanofibrils. Debby Sherman of DS Imaging is kindly acknowledged for TEM imaging. NR 38 TC 9 Z9 11 U1 9 U2 67 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2015 VL 22 IS 2 BP 1091 EP 1102 DI 10.1007/s10570-015-0563-9 PG 12 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA CD2AQ UT WOS:000350876300012 ER PT J AU Tandon, S AF Tandon, Sharad TI Taxation and Political Mobilization: Evidence from Andhra Pradesh SO ECONOMIC DEVELOPMENT AND CULTURAL CHANGE LA English DT Article ID REDISTRIBUTIVE POLITICS; US-HOUSE; INDIA; PROTECTION; INCENTIVES; COUNTRIES; ELECTIONS; POLICIES; ECONOMY C1 USDA, Washington, DC 20250 USA. RP Tandon, S (reprint author), USDA, Washington, DC 20250 USA. NR 48 TC 0 Z9 0 U1 1 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 0013-0079 EI 1539-2988 J9 ECON DEV CULT CHANGE JI Econ. Dev. Cult. Change PD APR PY 2015 VL 63 IS 3 BP 515 EP 549 DI 10.1086/679736 PG 35 WC Area Studies; Economics; Planning & Development SC Area Studies; Business & Economics; Public Administration GA CC8RI UT WOS:000350635400004 ER PT J AU Trappey, EF Khouryieh, H Aramouni, F Herald, T AF Trappey, Emily Frederick Khouryieh, Hanna Aramouni, Fadi Herald, Thomas TI Effect of sorghum flour composition and particle size on quality properties of gluten-free bread SO FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL LA English DT Article DE Sorghum; flour characterization; composition; particle size; gluten-free bread ID CELIAC-DISEASE; EXTRACTION RATE; DIETARY FIBER; WHEAT BREAD; BAKING; STARCH; DOUGH; CRUMB; GRAIN; BRAN AB White, food-grade sorghum was milled to flour of varying extraction rates (60%, 80%, and 100%) and pin-milled at different speeds (no pin-milling, low-speed, and high-speed) to create flours of both variable composition and particle size. Flours were characterized for flour composition, total starch content, particle size distribution, color, damaged starch, and water absorption. Bread was characterized for specific volume, crumb structure properties, and crumb firmness. Significant differences were found (P<0.05) in the composition of sorghum flours of varying extraction rate, most notably for fiber and total starch contents. Flour particle size and starch damage were significantly impacted by extraction rate and speed of pin-milling. Water absorption increased significantly with increasing extraction rate and pin-milling speed. Breads produced from 60% extraction flour had significantly higher specific volumes, better crumb properties, and lower crumb firmness when compared with all other extractions and flour types. The specific volume of bread slices ranged from 2.01mL/g (100% extraction, no pin-milling) to 2.54mL/g (60% extraction, low-speed pin-milling), whereas the firmness ranged from 553.28g (60% extraction, high-speed pin-milling) to 1096.26g (commercial flour, no pin-milling). The bread characteristics were significantly impacted by flour properties, specifically particle size, starch damage, and fiber content (P<0.05). C1 [Trappey, Emily Frederick; Aramouni, Fadi] Kansas State Univ, Inst Food Sci, Manhattan, KS 66506 USA. [Khouryieh, Hanna] Western Kentucky Univ, Food Proc & Technol Program, Bowling Green, KY 42101 USA. [Herald, Thomas] USDA ARS, Ctr Grain & Anim Hlth Res, Washington, DC 20250 USA. RP Khouryieh, H (reprint author), Western Kentucky Univ, Food Proc & Technol Program, Bowling Green, KY 42101 USA. EM hanna.khouryieh@wku.edu NR 45 TC 4 Z9 4 U1 6 U2 33 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1082-0132 EI 1532-1738 J9 FOOD SCI TECHNOL INT JI Food Sci. Technol. Int. PD APR PY 2015 VL 21 IS 3 BP 188 EP 202 DI 10.1177/1082013214523632 PG 15 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CD3KN UT WOS:000350977400003 PM 24519987 ER PT J AU Marcoux, HM Daniels, LD Gergel, SE Da Silva, E Gedalof, Z Hessburg, PF AF Marcoux, Helene M. Daniels, Lori D. Gergel, Sarah E. Da Silva, Eric Gedalof, Ze'ev Hessburg, Paul F. TI Differentiating mixed- and high-severity fire regimes in mixed-conifer forests of the Canadian Cordillera SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire history; Forest age structure; Fire exclusion; Larix occidentalis; Snag dynamics; Stand dynamics ID YELLOWSTONE-NATIONAL-PARK; PONDEROSA PINE FORESTS; INTERIOR BRITISH-COLUMBIA; DAKOTA BLACK-HILLS; OLD-GROWTH; LANDSCAPE PATTERNS; SOUTHERN CASCADES; TREE RECRUITMENT; ENGELMANN SPRUCE; WILDLAND FIRES AB Mixed-severity fire regimes are important drivers of forest dynamics, stand structural attributes, and regional and local landscape heterogeneity, but they remain poorly understood. We reconstructed site-level fire histories using fire scars and even-aged cohorts at 20 sites in two contiguous watersheds in southeastern British Columbia, Canada, a region that is particularly understudied. We compared stand composition and structural attributes (i.e., snag and veteran tree densities, tree size variability, and maximum tree size) at sites found to be mixed-severity, as well as those found to be high-severity after recent (<150 years) and older (>= 150 years) stand-replacing fires. We developed two forest indices capturing age-structure complexity and continuity to refine these comparisons. Eleven of 20 sites displayed mixed-severity fire histories and were located at elevations 600 m higher than previously described for this landscape. Tree species composition varied with disturbance history. Mixed-severity sites were dominated by Douglas-fir and western larch that regenerated after frequent low- to moderate-intensity fires, which created fire scars. Periodic moderate-severity fires generated some even-aged cohorts with surviving veteran trees. At higher-elevations, intense fires generated cohorts dominated by lodgepole pine. Subalpine fir dominated high-severity sites that last burned >250 years ago. Age structures at mixed- and older high-severity sites were of similar complexity, but could be differentiated using our index of age structure continuity. We found western larch to be a strong indicator of historical mixed-severity fire regimes. Western larch trees and stumps were only found at mixed-severity sites, and 84% of these individuals established within 15 years of antecedent fire scars. Snag densities were greatest at high-severity sites that burned >150 years ago, in contrast to expectations that mixed-severity sites would be more structurally complex. Tree size attributes were indistinguishable between mixed- and high-severity sites, although, subcanopy densities were particularly high (upwards of 5600 ha(-1)) having persisted since the last fire at most sites. Selective harvesting and fire suppression during the 20th century have homogenized contemporary forest structures in mid-elevation forests. An improved understanding of mixed-severity fire regimes is vital to determining whether forest resilience is compromised and where ecological restoration is warranted. (C) 2015 Elsevier B.V. All rights reserved. C1 [Marcoux, Helene M.; Daniels, Lori D.; Gergel, Sarah E.] Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V5Z 1M9, Canada. [Da Silva, Eric; Gedalof, Ze'ev] Univ Guelph, Dept Geog, Guelph, ON N1G 2W1, Canada. [Hessburg, Paul F.] USDA, FS, PNW Res Stn, Wenatchee, WA USA. RP Marcoux, HM (reprint author), British Columbia Inst Technol, Sustainable Resource Management Program, 3700 Willingdon Ave, Burnaby, BC V5G 3H2, Canada. EM marcouxhelene@gmail.com FU Natural Science and Engineering Council of Canada (NSERC); NSERC post-graduate scholarship; NSERC Discovery Grants FX This research was funded by a Strategic Project Grant from the Natural Science and Engineering Council of Canada (NSERC) to L.D. Daniels, an NSERC post-graduate scholarship to H.M. Marcoux and NSERC Discovery Grants to Z. Gedalof, S.E. Gergel, and L.D. Daniels. Thanks to O. Freeman, E. Paul-Limoges and M. Marcoux for assistance in the field; T. Martin, A. Baylis and C. Ankerson for help in the lab; K. Stuart-Smith and R. Harris for facilitating access to the study area. Thanks for helpful feedback from two anonymous reviewers. NR 93 TC 13 Z9 13 U1 6 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2015 VL 341 BP 45 EP 58 DI 10.1016/j.foreco.2014.12.027 PG 14 WC Forestry SC Forestry GA CD2XE UT WOS:000350941500006 ER PT J AU Morin, RS Liebhold, AM AF Morin, Randall S. Liebhold, Andrew M. TI Invasions by two non-native insects alter regional forest species composition and successional trajectories SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Adelges tsugae; Eastern hemlock; Tsuga canadensis; Beech bark disease; Fagus grandifolia; Growth and mortality rates ID BEECH BARK DISEASE; GYPSY-MOTH DEFOLIATION; EASTERN UNITED-STATES; WOOLLY ADELGID INFESTATION; SOUTHERN NEW-ENGLAND; AMERICAN BEECH; NORTH-AMERICA; VEGETATION DYNAMICS; TSUGA-CANADENSIS; HARDWOOD STANDS AB While invasions of individual non-native phytophagous insect species are known to affect growth and mortality of host trees, little is known about how multiple invasions combine to alter forest dynamics over large regions. In this study we integrate geographical data describing historical invasion spread of the hemlock woolly adelgid, Adelges tsugae, and beech scale, Cryptococcus fagisuga, with regional forest inventory data collected by the US Forest Service's Forest Inventory and Analysis program to quantify the individual and combined impacts of these pest species. This analysis indicates that regional impacts of these insects on their hosts occur surprisingly slow but act to change regional forest succession pathways. Because beech and hemlock commonly co-occur in eastern North American forests, invasions by the two pest species are altering the current and future composition of large forest regions through their impacts on these two late-successional species. Such results demonstrate how forest insect invasions can profoundly modify forest dynamic processes, resulting in long-term changes in forest ecosystems. Published by Elsevier B.V. C1 [Morin, Randall S.] USDA, Forest Serv, No Res Stn, Newtown Sq, PA 19073 USA. [Liebhold, Andrew M.] USDA, Forest Serv, No Res Stn, Morgantown, WV 26505 USA. RP Morin, RS (reprint author), USDA, Forest Serv, No Res Stn, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. EM rsmorin@fs.fed.us NR 65 TC 6 Z9 7 U1 9 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2015 VL 341 BP 67 EP 74 DI 10.1016/j.foreco.2014.12.018 PG 8 WC Forestry SC Forestry GA CD2XE UT WOS:000350941500008 ER PT J AU Knoot, TG Shea, ME Schulte, LA Tyndall, JC Nelson, MD Perry, CH Palik, BJ AF Knoot, T. G. Shea, M. E. Schulte, L. A. Tyndall, J. C. Nelson, M. D. Perry, C. H. Palik, B. J. TI Forest change in the Driftless Area of the Midwest: From a preferred to undesirable future SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest Inventory and Analysis; Mesophication; Oak forest; US Public Land Survey ID EASTERN UNITED-STATES; NORTH-AMERICA; LAND-USE; OAK FORESTS; USA; PRESETTLEMENT; DISTURBANCE; VEGETATION; LANDSCAPE; WISCONSIN AB In the midwestern and eastern U.S., oaks (Quercus spp.) have been a dominant component of forests for at least the last 10,000 years, providing vital habitat for numerous wildlife and plant species that have adapted to oak forest conditions. However, the current state of these oak systems, in which there has been a general lack of successful oak regeneration and recruitment and an increase in the relative dominance of mesophytic species, may be nearing critical thresholds. If reached, restoring oak systems through natural regeneration and other methods, such as prescribed fire, may become especially challenging if not impossible. An understanding of spatial variation in oak dominance over time can inform and potentially improve the efficacy of intervention strategies. Using Public Land Survey and Forest Inventory and Analysis (FIA) inventories, we evaluated changes in the composition of timberland across ecoregional subsections in the Driftless Area of the Midwest at three time periods (pre-settlement 1800s, 1990s, and 2000s). We identified an overall decrease in oak dominance, and particularly dominance of the white oak (Quercus alba L., Q. macrocarpa Michx., and Q. bicolor Willd.) species group since the pre-settlement era, and an increase in other eastern soft hardwoods. Within the last 20 years, both the red oak (Q. rubra L., Q. ellipsoidalis E.J. Hill and Q. velutina Lam.) and white oak species groups decreased in dominance, with an increase in hard maple-basswood (A. saccharum Marsh., A. nigra L, and Tilia americana L.) species group dominance, indicating further mesophication of forests in the region. However, we found a notable decrease in hard maple-basswood relative dominance within the small diameter class across most of the regions within the last 10-20 years, with an increase in dominance of other, non-oak, species. Our findings complement qualitative evidence from interviews with natural resource professionals from the region and offer further information on the potential for forest conversion to "undesirable" forest conditions, as identified as a source of concern by some professionals. There was spatial variation in these trends, however, with some pronounced differences across adjacent state boundaries. The variation in forest change across state boundaries suggests the role of state-level socioeconomic and policy factors in affecting forest conditions, and thus the potential for a targeted and timely approach to promoting preferred pathways of change. (C) 2014 Elsevier B.V. All rights reserved. C1 [Knoot, T. G.; Shea, M. E.; Schulte, L. A.; Tyndall, J. C.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Nelson, M. D.; Perry, C. H.] USDA Forest Serv, No Res Stn, Forest Inventory & Anal, St Paul, MN 55108 USA. [Palik, B. J.] USDA Forest Serv, No Res Stn, Ctr Res Ecosyst Change, Grand Rapids, MN 55744 USA. RP Knoot, TG (reprint author), Wisconsin Dept Nat Resources, Bur Sci Serv, Wildlife & Forestry Res Sect, 2801 Progress Rd, Madison, WI 53716 USA. EM tricia.knoot@wisconsin.gov RI Shea, Monika/A-8514-2016; OI Shea, Monika/0000-0001-6772-7114; Perry, Charles/0000-0001-9377-5996 FU USDA Forest Service - Northern Research Station; Wisconsin DNR, Bureau of Science Services; U.S. EPA - STAR FX This research was supported by funds from the USDA Forest Service - Northern Research Station and Iowa State University, with additional support from the Wisconsin DNR, Bureau of Science Services, during the final preparations of the manuscript. We greatly appreciate assistance by M. Hatfield, USDA Forest Service, who provided guidance and data analysis during the early development of this project. Assistance with data management was provided by A. Hand, and project guidance and helpful review comments were provided by B. Hutnik, B. Danielson, N. Grudens-Schuck, J. Miller, M. Rickenbach, A. Stoltman, L. Westphal, and two anonymous reviewers. T. Knoot was a research fellow with the U.S. EPA - STAR Graduate Fellowship Program during the initial development of this project. EPA has not officially endorsed this publication and the views expressed herein may not reflect the views of EPA. NR 61 TC 2 Z9 2 U1 7 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2015 VL 341 BP 110 EP 120 DI 10.1016/j.foreco.2014.12.013 PG 11 WC Forestry SC Forestry GA CD2XE UT WOS:000350941500012 ER PT J AU Jusino, MA Lindner, DL Banik, MT Walters, JR AF Jusino, Michelle A. Lindner, Daniel L. Banik, Mark T. Walters, Jeffrey R. TI Heart rot hotel: fungal communities in red-cockaded woodpecker excavations SO FUNGAL ECOLOGY LA English DT Article DE Cavity excavators and fungi; Fungal communities in excavations; Fungal communities in living trees; Red-cockaded woodpecker (Picoides borealis); Wood decay fungi ID CAVITY-TREE SELECTION; NESTING BIRDS; ECOSYSTEM; MANAGEMENT; HISTORY; FORESTS; DIVERSITY AB Tree-cavity excavators such as woodpeckers are ecosystem engineers that have potentially complex but poorly documented associations with wood decay fungi. Fungi facilitate cavity excavation by preparing and modifying excavation sites for cavity excavators. Associations between fungi and endangered red-cockaded woodpeckers (RCWs) are particularly interesting because these are the only birds that specialize in excavating into the heartwood of living pines, a process that takes years to complete. Using molecular methods, we examined fungal communities in complete and incomplete RCW excavations, and non-cavity control trees. In addition to finding a high diversity of fungi, we found three groupings of fungal communities corresponding to the three groups of trees sampled. We show that trees selected for cavity excavation by RCWs are infected by distinct fungal communities, and propose two hypotheses to explain this outcome: the bird facilitation hypothesis and the tree selection hypothesis. (C) 2014 Elsevier Ltd and The British Mycological Society. All rights reserved. C1 [Jusino, Michelle A.; Walters, Jeffrey R.] Virginia Tech, Dept Biol Sci, Blacksburg, VA 24061 USA. [Jusino, Michelle A.] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA. [Lindner, Daniel L.; Banik, Mark T.] US Forest Serv, Ctr Forest Mycol Res, Madison, WI 53726 USA. RP Jusino, MA (reprint author), Univ Wisconsin, Dept Med Microbiol & Immunol, 1550 Linden Dr, Madison, WI 53706 USA. EM mjusino@wisc.edu; dlindner@wisc.edu; mbanik@fs.fed.us; jrwalt@vt.edu FU U.S. Department of Defense, Marine Corps Base Camp Lejeune; Harold H. Bailey fund at Virginia Tech; US Forest Service, Northern Research Station; American Ornithologists' Union; Mycological Society of America; Society for Integrative & Comparative Biology; Virginia Tech Graduate Research and Development Program; U.S. Department of Defense, Strategic Environmental Research and Development Program (Defense Coastal/Estuarine Research Program) [RC-1471, RC-1413] FX We sincerely thank Kevin R. Rose for assistance in the field, Nicholas J. Brazee for assistance in the lab, and James Skelton for helpful comments and assistance with statistical analyses. We would also like to thank the VT Avian Ecology Lab and two anonymous reviewers for helpful comments on previous versions of this manuscript. Funding for fieldwork was provided by the U.S. Department of Defense, Marine Corps Base Camp Lejeune. Funding for analysis of samples was provided by the Harold H. Bailey fund at Virginia Tech and the US Forest Service, Northern Research Station. Further funding for this work was provided by the American Ornithologists' Union, the Mycological Society of America, the Society for Integrative & Comparative Biology, and the Virginia Tech Graduate Research and Development Program. Further support for MAJ was provided by the U.S. Department of Defense, Strategic Environmental Research and Development Program (RC-1471 and RC-1413, Defense Coastal/Estuarine Research Program). NR 52 TC 2 Z9 2 U1 8 U2 38 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1754-5048 EI 1878-0083 J9 FUNGAL ECOL JI Fungal Ecol. PD APR PY 2015 VL 14 BP 33 EP 43 DI 10.1016/j.funeco.2014.11.002 PG 11 WC Ecology; Mycology SC Environmental Sciences & Ecology; Mycology GA CD2VE UT WOS:000350936300004 ER PT J AU Kuske, CR Hesse, CN Challacombe, JF Cullen, D Herr, JR Mueller, RC Tsang, A Vilgalys, R AF Kuske, Cheryl R. Hesse, Cedar N. Challacombe, Jean F. Cullen, Daniel Herr, Joshua R. Mueller, Rebecca C. Tsang, Adrian Vilgalys, Rytas TI Prospects and challenges for fungal metatranscriptomics of complex communities SO FUNGAL ECOLOGY LA English DT Editorial Material ID TRANSCRIPTOME; METAGENOMICS C1 [Kuske, Cheryl R.; Hesse, Cedar N.; Challacombe, Jean F.; Mueller, Rebecca C.] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA. [Cullen, Daniel] Univ Wisconsin, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Herr, Joshua R.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. [Tsang, Adrian] Concordia Univ, Ctr Struct & Funct Genom, Montreal, PQ H4B 1R6, Canada. [Vilgalys, Rytas] Duke Univ, Biol Sci, Durham, NC 27708 USA. RP Kuske, CR (reprint author), Los Alamos Natl Lab, Biosci Div, M888, Los Alamos, NM 87545 USA. OI Herr, Joshua/0000-0003-3425-292X NR 15 TC 7 Z9 7 U1 2 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1754-5048 EI 1878-0083 J9 FUNGAL ECOL JI Fungal Ecol. PD APR PY 2015 VL 14 BP 133 EP 137 DI 10.1016/j.funeco.2014.12.005 PG 5 WC Ecology; Mycology SC Environmental Sciences & Ecology; Mycology GA CD2VE UT WOS:000350936300016 ER PT J AU Jiang, T Skyllberg, U Wei, SQ Wang, DY Lu, S Jiang, ZM Flanagan, DC AF Jiang, Tao Skyllberg, Ulf Wei, Shiqiang Wang, Dingyong Lu, Song Jiang, Zhenmao Flanagan, Dennis C. TI Modeling of the structure-specific kinetics of abiotic, dark reduction of Hg(II) complexed by O/N and S functional groups in humic acids while accounting for time-dependent structural rearrangement SO GEOCHIMICA ET COSMOCHIMICA ACTA LA English DT Article ID NATURAL ORGANIC-MATTER; X-RAY-ABSORPTION; REDUCED SULFUR GROUPS; SUBSTANCES RIO-NEGRO; K-EDGE XANES; DIVALENT MERCURY; SPECTROSCOPIC EVIDENCE; QUANTITATIVE-ANALYSIS; ANOXIC ENVIRONMENTS; ELEMENTAL MERCURY AB Dark reduction of Hg(II) to Hg(0) in deep waters, soils and sediments accounts for a large part of legacy Hg recycling back to the atmosphere. Natural organic matter (NOM) plays a dual role in the process, acting as an electron donor and complexation agent of Hg(II). Experimental determination of rates of dark Hg(II) reduction is complicated by the simultaneously ongoing kinetics of Hg(II) rearrangement from the abundant, relatively weakly bonding RO/N (carboxyl, amino) groups in NOM to the much stronger bonding RSH (thiol) group. In this study, kinetics of the rearrangement are accounted for and we report rates of dark Hg(II) reduction for two molecular structures in presence of humic acids (HA) extracted from three different sources. Values on the pseudo first-order rate constant for the proposed structure Hg(OR)(2) (k(red) (Hg(OR)2)) were 0.18, 0.22 and 0.35 h(-1) for Peat, Coal and Soil HA, respectively, and values on the constant for the proposed structure RSHgOR (k(red) (RSHgOR)) were 0.003 and 0.006 h(-1) for Peat and Soil HA, respectively. The Hg(SR)(2) structure is the thermodynamically most stable, but the limited time of the experiment (53 h) did not allow for a determination of the rate of the very slow reduction of Hg(II) in this structure. For two out of three HA samples the concentration of RSH groups optimized by the kinetic model (0.6 x 10(-3) RSH groups per C atoms) was in good agreement with independent estimates provided by sulfur X-ray absorption near-edge spectroscopy (S XANES). Experiments conducted at varying concentrations of Hg(II) and HA demonstrated a positive relationship between Hg(II) reduction and concentrations of specific Hg(II) structures and electron donor groups, suggesting first order in each of these two components. The limitation of the Hg(II) reduction by electron donating groups of HA, as represented by the native reducing capacity (NRC), was demonstrated for the Coal HA sample. Normalization to NRC resulted in pseudo second-order rate constants (k(red) (Hg(OR)2/NRC) = 0.012 h(-1), k(red) (RSHgOR/NRC) = 1.9 - 2.1 x 10(-4) h(-1)) that were very similar for Soil and Peat HA. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Jiang, Tao; Wei, Shiqiang; Wang, Dingyong; Lu, Song; Jiang, Zhenmao] Southwest Univ, Dept Environm Sci & Engn, Coll Resources & Environm, Key Lab,Three Gorges Reservoir Reg Eco Environm,M, Chongqing, Peoples R China. [Jiang, Tao; Wei, Shiqiang; Wang, Dingyong; Lu, Song; Jiang, Zhenmao] Chongqing Key Lab Agr Resources & Environm, Chongqing, Peoples R China. [Jiang, Tao; Skyllberg, Ulf] Swedish Univ Agr Sci, Dept Forest Ecol & Management, SE-90183 Umea, Sweden. [Flanagan, Dennis C.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN USA. RP Skyllberg, U (reprint author), Swedish Univ Agr Sci, Dept Forest Ecol & Management, SE-90183 Umea, Sweden. EM ulf.skyllberg@slu.se; sqwei@swu.edu.cn OI Skyllberg, Ulf/0000-0001-6939-8799 FU National Science Foundation of China [41403079, 40971147]; National Key Basic Research Program of China (973 Program) [2013CB430004]; China Postdoctoral Science Foundation [2013M542238]; Fundamental Research Funds for the Central Universities [XDJK2013C151]; MAX-lab, Lund, Sweden FX We gratefully acknowledge the financial support from the National Science Foundation of China (41403079), National Key Basic Research Program of China (973 Program) (2013CB430004), the China Postdoctoral Science Foundation (2013M542238), National Science Foundation of China (40971147) and the Fundamental Research Funds for the Central Universities (XDJK2013C151). We acknowledge funding from MAX-lab, Lund, Sweden, and support from the staff at beamline I-811 during S XANES data collection. NR 73 TC 8 Z9 8 U1 8 U2 42 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0016-7037 EI 1872-9533 J9 GEOCHIM COSMOCHIM AC JI Geochim. Cosmochim. Acta PD APR 1 PY 2015 VL 154 BP 151 EP 167 DI 10.1016/j.gca.2015.01.011 PG 17 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA CC7PP UT WOS:000350560800010 ER PT J AU Kim, K Gesch, RW AF Kim, K. Gesch, R. W. TI Macro and Microminerals of four Cuphea genotypes grown across the upper Midwest USA SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Fatty acids; Macronutrients; Micronutrients; Heavy metals; ICP ID MANAGEMENT; ALFALFA; OIL AB Cuphea seed oil can be used for many purposes from motor oil and cosmetic components to jet fuel, because its seeds are a rich source of medium-chain fatty acids (C8:0 to C14:0). Processing cuphea oil to biofuel and other bioproducts of high quality can depend on the heavy metal content of its seed. However, little is known about the macro and micromineral content of its seed. The objective of this study was to evaluate mineral content of cuphea, especially heavy metal content for processing the oil into biofuels/bioproducts and to determine how this may differ across environments. Four cuphea genotypes were evaluated; two of which were semi-domesticated genotypes [PSR23 and HC-10 (Cuphea viscosissimaJacq. x C. lanceolata W.T. Aiton)] and two were wild species (Cuphea wrightii and C. viscosisima (VS-6-CPR-1). The study was conducted at four locations in North Dakota (ND), Minnesota (MN), Iowa (IA), and Illinois (IL). The soil was a Perella silty clay loam at Prosper, ND, a Barnes loam at Morris, MN, a Clarion loam at Ames, IA, and an Osco silt loam at Macomb, IL PSR23 and HC-10 were directly-seeded in the field and the two wild species were transplanted. All cuphea seeds contained some heavy metals. Although phosphorus (P) and potassium (K) were equally fertilized at all sites, the concentrations of P and K in seeds of PSR23 and HC-10 tended to be lower at the MN site than IA and IL sites, while the lowest P concentration was observed at the MN site for VS-6 and C. wrightii. Among calcium (Ca), magnesium (Mg), and sodium (Na) in cuphea seeds, sodium had the lowest concentration. The average concentrations for microminerals ranged from 40 to 53 mg kg(-1) for zinc (Zn), from 11.9 to 14.1 mg kg(-1) for copper (Cu). These concentrations are low compared to those found in many livestock feeds. Lead (Pb) and vanadium (V) were sporadically observed at a few locations. Cobalt (Co) was only detected for VS-6 at IA and C. wrightii at IA and MN, while cadmium (Cd) was only found for HC-10 at ND, PSR23 at IA, and C wrightii at ND. These results suggest that the concentrations of macro cuphea seeds varied by genotype and environment. This information will be useful for those industries interested in processing cuphea seed oil for manufacturing biofuels, cosmetics, and other specialty chemicals. Published by Elsevier B.V. C1 [Kim, K.] Mokpo Natl Univ, Dept Hort Sci, Mu An, South Korea. [Gesch, R. W.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Kim, K (reprint author), Jeon Nam Mu An Cheong Gye Young San Ro 1666, Seoul, South Korea. EM rollingkim@mokpo.ac.kr FU Energy & Environmental Research Center, University of North Dakota, through the Defense Advanced Research Projects Agency (DARPA) FX The authors thank Joe Boots, Fred Lutzi, and Iry Larsen for their expert field assistance and Jana Rinke for helping with the ICP analysis. We also acknowledge funding by the Energy & Environmental Research Center, University of North Dakota for cuphea research, through a grant from the Defense Advanced Research Projects Agency (DARPA). NR 25 TC 0 Z9 0 U1 3 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2015 VL 66 BP 38 EP 43 DI 10.1016/j.indcrop.2014.12.030 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CD2TW UT WOS:000350932900005 ER PT J AU Vaughn, SF Kenar, JA Eller, FJ Moser, BR Jackson, MA Peterson, SC AF Vaughn, Steven F. Kenar, James A. Eller, Fred J. Moser, Bryan R. Jackson, Michael A. Peterson, Steven C. TI Physical and chemical characterization of biochars produced from coppiced wood of thirteen tree species for use in horticultural substrates SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biochar; Coppice; Horticultural substrates; Carbon sequestration ID SHORT-ROTATION COPPICE; GROWTH; PLANTATIONS; PYROLYSIS; CHARCOAL; POPLAR; CARBON; SOILS; LAND AB Seven-year-old coppiced shoots from thirteen species of both native and non-native trees and shrubs were harvested, dried, and pyrolyzed to produce biochars for potential use in horticultural substrates. Several chemical and physical characteristics of the biochars were determined. There were slight differences among the parent coppiced shoots as to heats of combustion and biochar yields, with gray dogwood shoots having the highest values for both parameters. Surface areas, micropore surface areas, and bulk densities varied to a greater degree among the species. All biochars had very strongly basic pH values (>9.0), although electrical conductivity values varied greatly, appearing to correlate with levels of potassium found in the biochars. Ash content of the biochars was similar, and using the guidelines for O/C, H/C, and surface area, all biochar samples meet standards for utilization as soil amendments. Peak moisture sorption of the biochars was directly proportional to surface areas. From the results of this study, while there were differences in chemical and physical structures of the biochars examined, most of these tree species would be suitable for use in horticultural applications. Other factors such as, rate of growth of the coppiced shoots, level of fertility management, and resistance to herbivore browsing are important as well and can be used to determine the optimal species for a given geographic region. Published by Elsevier B.V. C1 [Vaughn, Steven F.; Kenar, James A.; Eller, Fred J.] USDA ARS, Funct Foods Res, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Moser, Bryan R.] USDA ARS, Biooils Res, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Jackson, Michael A.] USDA ARS, Renewable Prod Technol Res, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Peterson, Steven C.] USDA ARS, Plant Polymer Res, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Vaughn, SF (reprint author), USDA ARS, Funct Foods Res, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Steven.Vaughn@ars.usda.gov NR 42 TC 5 Z9 5 U1 3 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2015 VL 66 BP 44 EP 51 DI 10.1016/j.indcrop.2014.12.026 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA CD2TW UT WOS:000350932900006 ER PT J AU Royo-Esnal, A Necajeva, J Torra, J Recasens, J Gesch, RW AF Royo-Esnal, Aritz Necajeva, Jevgenija Torra, Joel Recasens, Jordi Gesch, Russ W. TI Emergence of field pennycress (Thlaspi arvense L.): Comparison of two accessions and modelling SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Brassicaceae; Hydrothermal time; Pennycress; Oilseed species; Photohydrothermal time; Rare weed ID SEEDLING EMERGENCE; ENVIRONMENTAL-CONTROL; WEED; GERMINATION; CRUCIFERAE; BIODIESEL; CROPS; TOOLS; SOIL; OIL AB Many weed species are becoming rare due to intense agricultural management, which leads to a decrease of biodiversity in agroecosystems. Cultivating some of these species for their oilseed content may help preserve them while profiting agronomically. Thlaspi arvense is one of these species with potential as an industrial crop. The aim of this work was to develop a model to describe the emergence of this species, and that can help to make decisions for its management, whether for conservation or production purposes. The emergence of two accessions of T. arvense, one from Spain and the other from USA, sown in Spain (Almenar) and USA (Morris), over two seasons (2011-12 and 2012-13) and in Riga (Latvia) over one season (2012), was followed to compare patterns and extent, as well as to develop emergence prediction models based on hydrothermal time (HTT) and photohydrothermal time (PhHTT). For the USA accession, the percentage of seeds that emerged was significantly higher than that of the Spanish accession. Both accessions presented two emergence peaks (autumn winter and spring) in both localities, but while these peaks could be considered as two different flushes in Almenar for both accessions, they appeared to be a single flush disrupted by low winter temperatures in Morris. An HTT-based model was applicable for both accessions with less precision than the PhHTT-based one, which was more accurate in most cases but failed in certain circumstances for the USA accession. The differences in emergence percentage among accessions suggest that some accessions might be more amenable to being used as a crop. The two models developed in this work predicted the emergence of both accessions of T. arvense quite accurately. The inclusion of photoperiod in the hydrothermal time equation, creating a new unit that we have called photohydrothermal time, offers a possibility to obtain more accurate models. (C) 2014 Elsevier B.V. All rights reserved. C1 [Royo-Esnal, Aritz; Torra, Joel; Recasens, Jordi] Univ Lleida, Dept Hortofruticultura Bot & Jardineria, Agrotecnio, Lleida 25198, Spain. [Necajeva, Jevgenija] Univ Latvia, Dept Plant Physiol, LV-1586 Riga, Latvia. [Gesch, Russ W.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Royo-Esnal, A (reprint author), Univ Lleida, Dept Hortofruticultura Bot & Jardineria, Agrotecnio, Alcalde Rovira Roure 191, Lleida 25198, Spain. EM aritz@hbj.udl.cat RI Torra, Joel/B-3959-2011; Royo-Esnal, Aritz/B-4174-2011; Recasens, Jordi/B-3970-2011 OI Torra, Joel/0000-0002-8666-6780; Royo-Esnal, Aritz/0000-0003-2534-8372; Recasens, Jordi/0000-0002-8799-9958 FU Ministerio de Educacion y Cienciade Espana [AGL2010-22084-C02-01] FX This work was supported by the Ministerio de Educacion y Cienciade Espana (AGL2010-22084-C02-01). We thank the Botanical Garden of the University of Latvia for letting us set the experiment in their court. The group from Lleida thanks Nuria Moix, Ana Isabal, Jordi Rey-Ana Banos and Eva Edo for their help in the field. We also thank Joe Boots for his expert field assistance at the Morris, Minnesota field site. Finally, we thank Dr Frank Forcella for his valuable suggestions to improve this manuscript. NR 29 TC 7 Z9 7 U1 3 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2015 VL 66 BP 161 EP 169 DI 10.1016/j.indcrop.2014.12.010 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA CD2TW UT WOS:000350932900022 ER PT J AU Isbell, TA Evangelista, R Glenn, SE Devore, DA Moser, BR Cermak, SC Rao, S AF Isbell, Terry A. Evangelista, Roque Glenn, Steve E. Devore, Drew A. Moser, Bryan R. Cermak, Steven C. Rao, Serin TI Enrichment of erucic acid from pennycress (Thlaspi arvense L.) seed oil SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Pennycress; Thlaspi arvense; Erucic Acid; Distillation; Soap crystallization ID PURIFICATION AB Pennycress (Thlaspi arvense L.) is a winter annual that has a wide geographic distribution and a growth habit that makes it suitable for an off-season rotation between corn and soybeans in much of the Midwestern United States. Pennycress seed contains 36% oil with 36.6% erucic acid content There are a number of markets that pennycress could supply from an enriched erucic fatty acid fraction. Erucic acid was enriched using two independent separation methods; vacuum distillation of fatty acids or methyl esters and fractional crystallization of potassium soaps directly from the triglyceride. Fractional crystallization provided the highest level of purity, yielding an 87% erucic enriched fraction but in low theoretical recovery of 23% when ethanol was used as the crystallization solvent. A higher theoretical erucic recovery (59%) was obtained when methanol was used as a solvent yielding a 71% enriched erucic acid fraction. This method utilized a mixed solvent (90:10 methanol/water) for crystallization with a 5:1 solvent to analyte ratio. In an independent study, molecular distillation of the fatty acid methyl esters at 90 degrees C, 7 Pa of pressure enriched the erucic acid content to 67.1% in a single pass and could be further enriched to 71.6% with a second pass with an overall mass balance for this double distilled fraction of 43.2%. Distillation of the fatty acids was similar to the methyl esters yielding a fraction that contained 69.0% erucic with an overall mass balance of this fraction of 38.4% after two distillations. All three enriched fractions (feed, distilled FAME and mother liquor) were suitable for biodiesel; 40 degrees C viscosity range 4.142-5.509, 100 degrees C viscosity range of 1.713-2.095. Pour points were improved from the feed of -18 degrees C to -27 degrees C in the mother liquor fraction and remained the same in the distillate at -18 degrees C. HFRR wear scars ranged from 120 to 177 mu m across all fractions. Published by Elsevier B.V. C1 [Isbell, Terry A.; Evangelista, Roque; Glenn, Steve E.; Devore, Drew A.; Moser, Bryan R.; Cermak, Steven C.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Rao, Serin] Arvens Technol Inc, Peoria, IL 61606 USA. RP Isbell, TA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM terry.isbell@ars.usda.gov OI Isbell, Terry/0000-0002-7239-776X FU Biotechnology Research Development Corporation [58-3K95-2-1544] FX The authors thank Jeff Forrester and Billy Deadmond for expelling pennycress oil, Ben Lowery for synthesis of pennycress methyl esters and Benetria Banks for physical property testing. The authors thank Biotechnology Research Development Corporation for providing funding for this work under the Cooperative Research and Development Agreement 58-3K95-2-1544. NR 20 TC 2 Z9 2 U1 2 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2015 VL 66 BP 188 EP 193 DI 10.1016/j.indcrop.2014.12.050 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CD2TW UT WOS:000350932900025 ER PT J AU Garcia-Gonzalez, MC Vanotti, MB Szogi, AA AF Garcia-Gonzalez, M. C. Vanotti, M. B. Szogi, A. A. TI Recovery of ammonia from swine manure using gas-permeable membranes: Effect of aeration SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Gas-permeable membranes; Ammonia recovery; Nutrient recovery; Swine manure; Aeration effect ID WASTE-WATER; ANAEROBIC-DIGESTION; DAIRY MANURE; PHOSPHORUS; REMOVAL; SEPARATION; SLUDGE; SLURRY AB The gas-permeable membrane process can recover ammonia from manure, reducing pollution whilst converting ammonia into an ammonium salt fertilizer. The process involves manure pH control to increase ammonium (NH4+) recovery rate that is normally carried out using an alkali. In this study a new strategy to avoid the use of alkali was tested applying low-rate aeration and nitrification inhibition. The wastewater used was raw swine manure with 2390 mg NF4+-N/L. Results showed that aeration increased pH above 8.5 allowing quick transformation of NH4+ into gaseous ammonia (NH3) and efficient recovery by permeation through the submerged membrane. The overall NH4+ recovery obtained with aeration was 98% and ammonia emissions losses were less than 1.5%. The new approach can substitute large amounts of alkali chemicals needed to obtain high NH4+ recovery with important economic and environmental savings. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Garcia-Gonzalez, M. C.] Agr Technol Inst Castilla & Leon ITACyL, Valladolid 47071, Spain. [Vanotti, M. B.; Szogi, A. A.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. RP Garcia-Gonzalez, MC (reprint author), Agr Technol Inst Castilla & Leon ITACyL, Carretera Burgos,Km 119, Valladolid 47071, Spain. EM gargonmi@itacyl.es OI Garcia Gonzalez, Maria Cruz/0000-0002-9860-363X FU INIA/FEDER Project "Transferencia de tecnologia para la gestion de residuos ganaderos y de la industria agro-alimentaria de Castilla y Leon" [CC09-072]; USDA-ARS Project "Innovative Animal Manure Treatment Technologies for Enhanced Environmental Quality" [6657-13630-001-00D] FX This work, has been funded by the INIA/FEDER Project CC09-072 "Transferencia de tecnologia para la gestion de residuos ganaderos y de la industria agro-alimentaria de Castilla y Leon". Cooperation with USDA-ARS Project 6657-13630-001-00D "Innovative Animal Manure Treatment Technologies for Enhanced Environmental Quality" is gratefully acknowledged. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 23 TC 4 Z9 4 U1 1 U2 33 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD APR 1 PY 2015 VL 152 BP 19 EP 26 DI 10.1016/j.jenvman.2015.01.013 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CC7AF UT WOS:000350519400003 PM 25602923 ER PT J AU Briske, DD Zhao, ML Han, GD Xiu, CB Kemp, DR Willms, W Havstad, K Kang, L Wang, ZW Wu, JG Han, XG Bai, YF AF Briske, David D. Zhao, Mengli Han, Guodong Xiu, Changbai Kemp, David R. Willms, Walter Havstad, Kris Kang, Le Wang, Zhongwu Wu, Jianguo Han, Xingguo Bai, Yongfei TI Strategies to alleviate poverty and grassland degradation in Inner Mongolia: Intensification vs production efficiency of livestock systems SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Review DE Pastoralism; Pastoral livelihoods; Poverty trap; Social-ecological systems; Sustainability ID RANGELAND DEGRADATION; CHINA; MANAGEMENT; ENVIRONMENT; STEPPE; ECOSYSTEMS; PASTURE AB Semi-nomadic pastoralism was replaced by sedentary pastoralism in Inner Mongolia during the 1960's in response to changes in land use policy and increasing human population. Large increases in numbers of livestock and pastoralist households (11- and 9-fold, respectively) during the past 60 yrs have variously degraded the majority of grasslands in Inner Mongolia (78 M ha) and jeopardize the livelihoods of 24 M human inhabitants. A prevailing strategy for alleviating poverty and grassland degradation emphasizes intensification of livestock production systems to maintain both pastoral livelihoods and large livestock numbers. We consider this strategy unsustainable because maximization of livestock revenue incurs high supplemental feed costs, marginalizes net household income, and promotes larger flock sizes to create a positive feedback loop driving grassland degradation. We offer an alternative strategy that increases both livestock production efficiency and net pastoral income by marketing high quality animal products to an increasing affluent Chinese economy while simultaneously reducing livestock impacts on grasslands. We further caution that this strategy be designed and assessed within a social-ecological framework capable of coordinating market expansion for livestock products, sustainable livestock carrying capacities, modified pastoral perceptions of success, and incentives for ecosystem services to interrupt the positive feedback loop that exists between subsistence pastoralism and grassland degradation in Inner Mongolia. (C) 2015 Published by Elsevier Ltd. C1 [Briske, David D.; Wang, Zhongwu] Texas A&M Univ, Ecosyst Sci & Management, College Stn, TX USA. [Zhao, Mengli; Han, Guodong; Xiu, Changbai] Inner Mongolian Agr Univ, Ecol & Environm Sci, Hohhot, Peoples R China. [Kemp, David R.] Univ Sydney, Charles Sturt Univ, Orange, NSW, Australia. [Willms, Walter] Agr & Agri Food Canada, Lethbridge, AB, Canada. [Havstad, Kris] USDA ARS, Jornada Expt Range, Las Cruces, NM USA. [Kang, Le] Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China. [Wu, Jianguo] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Wu, Jianguo] Arizona State Univ, Global Inst Sustainabil, Tempe, AZ USA. [Wu, Jianguo] Inner Mongolia Univ, Sino US Ctr Conservat Energy & Sustainabil Sci, Hohhot, Peoples R China. [Han, Xingguo; Bai, Yongfei] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. RP Zhao, ML (reprint author), Inner Mongolian Agr Univ, Ecol & Environm Sci, Hohhot, Peoples R China. EM menglizhao@yahoo.com RI Han, Xingguo/K-7552-2016 OI Han, Xingguo/0000-0002-1836-975X FU Australian Centre for International Agricultural Research, Hohhot, China; Institute of Botany, Chinese Academy of Science, Beijing; Chinese National Key Fundamental Research and Development Fund [2007CB106800]; Chinese National Nature Science Foundation [30860196, 31070414]; National Key Laboratory of Grassland Science and Range Resources; DRK FX These concepts originated from a workshop "Improving Grassland and Incomes in Western China" sponsored by the Australian Centre for International Agricultural Research, Hohhot, China, June 2008, and a symposium "Regional and Global Networks of Grassland Research: Issues and Perspectives" sponsored by the Institute of Botany, Chinese Academy of Science, Beijing, August 2009. Project supported by Chinese National Key Fundamental Research and Development Fund (No.2007CB106800), Chinese National Nature Science Foundation (No. 30860196, 31070414) and National Key Laboratory of Grassland Science and Range Resources. The Australian Centre for International Agricultural Research, the Australian Department of Agriculture, Fisheries and Forestry and the Australian Greenhouse Office funded DRK. NR 41 TC 4 Z9 5 U1 8 U2 76 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD APR 1 PY 2015 VL 152 BP 177 EP 182 DI 10.1016/j.jenvman.2014.07.036 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA CC7AF UT WOS:000350519400021 PM 25687702 ER PT J AU Bedinger, DH Goldfine, ID Corbin, JA Roell, MK Adams, SH AF Bedinger, Daniel H. Goldfine, Ira D. Corbin, John A. Roell, Marina K. Adams, Sean H. TI Differential Pathway Coupling of the Activated Insulin Receptor Drives Signaling Selectivity by XMetA, an Allosteric Partial Agonist Antibody SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID GLUCOSE-LOWERING THERAPIES; TISSUE-SPECIFIC EXPRESSION; IMPROVES GLYCEMIC CONTROL; COOH-TERMINAL TRUNCATION; PROTEIN-KINASE; MONOCLONAL-ANTIBODY; HIGH-AFFINITY; CANCER-RISK; BINDING; GROWTH AB The monoclonal antibody XMetA is an allosteric partial agonist of the insulin receptor (IR), which activates the metabolic Akt kinase signaling pathway while having little or no effect on the mitogenic extracellular signal-regulated kinase (ERK) signaling pathway. To investigate the nature of this selective signaling, we have conducted a detailed investigation of XMetA to evaluate specific phosphorylation and activation of IR, Akt, and ERK in Chinese hamster ovary cell lines expressing either the short or long isoform of the human IR. Insulin activated both pathways, but the phosphorylation of Akt was more sensitive to the hormone than the phosphorylation of ERK. Maximally effective concentrations of XMetA elicited phosphorylation patterns similar to 40-100 pM insulin, which were sufficient for robust Akt phosphorylation, but had little effect on ERK phosphorylation. These data indicate that the preferential signaling of XMetA is due to an innate difference in pathway sensitivity of Akt versus ERK responses to IR activation and partial agonism by XMetA, rather than a separate pathway-biased mechanism. The metabolic selectivity of partial IR agonists like XMetA, if recapitulated in vivo, may be a desirable feature of therapeutic agents designed to regulate blood glucose levels while minimizing undesirable outcomes of excessive IR mitogenic activation. C1 [Bedinger, Daniel H.; Goldfine, Ira D.; Corbin, John A.; Roell, Marina K.] Xoma Corp, Berkeley, CA 94710 USA. [Adams, Sean H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Adams, Sean H.] Dept Nutr, Davis, CA USA. [Bedinger, Daniel H.; Adams, Sean H.] Univ Calif Davis, Mol Cellular & Integrat Physiol Grad Grp, Davis, CA 95616 USA. RP Adams, SH (reprint author), Arkansas Childrens Nutr Ctr, 15 Childrens Way, Little Rock, AR 72202 USA. EM shadams@uams.edu FU Cooperative Research and Development Agreement [CRADA] [58-3K95-1-1497]; USDA-ARS Intramural Project [5306-51530-019-00D] FX This work was funded by a Cooperative Research and Development Agreement [CRADA 58-3K95-1-1497] between XOMA and United States Department of Agriculture-Agricultural Research Service (USDA-ARS), as well as through a USDA-ARS Intramural Project [5306-51530-019-00D]. USDA is an equal opportunity provider and employer. D.H.B., I.D.G., J.A.C., and M.K.R. are employees of XOMA (US), LLC. No other potential conflicts of interest relevant to this article are reported. S.H.A. has no conflict of interest to declare. NR 62 TC 5 Z9 5 U1 1 U2 6 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 0022-3565 EI 1521-0103 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR PY 2015 VL 353 IS 1 BP 35 EP 43 DI 10.1124/jpet.114.221309 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA CD4HP UT WOS:000351043600005 PM 25613982 ER PT J AU Prasifka, JR AF Prasifka, Jarrad R. TI Variation in the number of capitate glandular trichomes in wild and cultivated sunflower germplasm and its potential for use in host plant resistance SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE antibiosis; Asteraceae; breeding; Melanagromyza minimoides; sesquiterpene lactones ID HELIANTHUS-ANNUUS L; SESQUITERPENE LACTONES; HOMOEOSOMA-ELECTELLUM; MOTH LEPIDOPTERA; PYRALIDAE; ASSOCIATIONS; NOCTUIDAE; HOMOPTERA; TOMATO; PESTS AB The capitate glandular trichomes of wild sunflowers (Helianthus spp.) are considered to be effective defence components that act against some herbivorous insects, but cultivated sunflowers are reportedly deficient in glandular trichomes. To investigate whether glandular trichomes have a role in the protection of cultivated sunflowers against insects, in the present study, Helianthus annuus L. accessions were grown to quantify glandular trichome density in wild and cultivated germplasm types and assess potential anti-insect effects of terpenoids in the glandular trichomes of cultivated sunflowers. Evaluation revealed that capitate glandular trichomes are often abundant in cultivated sunflowers; relative to wild H. annuus, inbred maintainer (HA) lines have similar numbers of glandular trichomes per floret, while commercial hybrids have only approximate to 20% fewer trichomes when compared with wild sunflowers. In the laboratory assay, it was found that glandular trichome extracts increased the mortality rates of sunflower moth, Homoeosoma electellum (Hulst), larvae exposed from the neonatal stage to 9 d. In the surviving larvae, the extracts significantly reduced larval mass and head capsule width. Though there are limitations to the value of glandular trichomes for host plant resistance, the feeding deterrent or toxic effects of sesquiterpene lactones and diterpenes in sunflower glandular trichomes are not limited to sunflower moth larvae, suggesting a potential for resistance to other sunflower insect pests. Additional research is required to understand the inheritance and value of glandular trichomes in commercial sunflower germplasm and how the composition of terpenoids in the glandular trichomes of wild H. annuus may differ from that in cultivated material. C1 USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. RP Prasifka, JR (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM jarrad.prasifka@ars.usda.gov NR 29 TC 2 Z9 3 U1 2 U2 16 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 EI 1479-263X J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD APR PY 2015 VL 13 IS 1 BP 68 EP 74 DI 10.1017/S1479262114000653 PG 7 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CC8QM UT WOS:000350633200008 ER PT J AU Seiler, GJ AF Seiler, Gerald J. TI Comparison of fatty acid composition of oil from original and regenerated populations of wild Helianthus species SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE genebank maintenance; genetic resources; linoleic acid; oil quality; oleic acid; sunflower species ID MORPHOLOGICAL-CHARACTERISTICS; GENETIC RESOURCE; ANNUAL SUNFLOWER; SEED OIL; GERMPLASM; TEMPERATURE; ASTERACEAE; ACHENES AB Monitoring and protecting germplasm in genebanks using in situ collections while preserving its original genetic integrity is a priority of germplasm curation. Many germplasm accessions need to be regenerated due to their demand and/or seed condition. The regeneration of wild Helianthus (sunflower) species poses several challenges due to the diversity of 53 wild species. Fatty acid composition of sunflower oil is an important quality factor for the crop. Since oil quality is environmentally influenced, and evaluation of this trait is usually performed on oil from achenes from the original accessions of wild sunflower species, we conducted a study on 72 accessions of eight annual and four perennial taxa of wild sunflower species to compare the oil quality of the original accessions and those regenerated for genebank maintenance. The results showed that the fatty acid composition profiles of achenes from the original and regenerated accessions are not the same. It seems that selection for specific fatty acids in several species will require the analysis of both populations to identify germplasm accessions for use in breeding programmes. It should be borne in mind that accessions of wild species are open-pollinated segregating populations, so one would expect some variability in each succeeding generation. While there may be differences between the original and regenerated accessions, the interrelationships of fatty acids are generally similar in wild and cultivated sunflower species, so there should be no detrimental effects on oil quality when using the wild species for other traits. As more regenerated accessions become available, a more precise relationship between the original and regenerated accessions should emerge. C1 ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. RP Seiler, GJ (reprint author), ARS, USDA, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM gerald.seiler@ars.usda.gov FU USDA-ARS CRIS [5442-21000-039-00D] FX The author acknowledges Ms Marjorie Olson, USDA-ARS, Fargo, ND for providing expert technical assistance and Ms Mary Brothers (former curator of the wild sunflower species collection), USDA-ARS, North Central Regional Plant Introduction Station, Ames, IA, USA for providing the samples for this study. This study was supported by the USDA-ARS CRIS Project No. 5442-21000-039-00D. Mention of trade names or commercial products in this study is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 25 TC 0 Z9 0 U1 4 U2 13 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 EI 1479-263X J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD APR PY 2015 VL 13 IS 1 BP 83 EP 89 DI 10.1017/S1479262114000677 PG 7 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CC8QM UT WOS:000350633200010 ER PT J AU Kolmer, JA Kabdulova, MG Mustafina, MA Zhemchuzhina, NS Dubovoy, V AF Kolmer, J. A. Kabdulova, M. G. Mustafina, M. A. Zhemchuzhina, N. S. Dubovoy, V. TI Russian populations of Puccinia triticina in distant regions are not differentiated for virulence and molecular genotype SO PLANT PATHOLOGY LA English DT Article DE brown rust; Lr resistance genes; simple sequence repeat; specific virulence; Triticum aestivum ID LEAF RUST; GENETIC DIFFERENTIATION; MICROSATELLITE LOCI; COMMON WHEAT; RESISTANCE; DIVERSITY; AMERICA AB The objective of this study was to determine whether genetically distinct groups of Puccinia triticina are present in four regions of the Russian Federation. Collections of P.triticina were obtained from the Central, North Caucasus, Volga and West Siberia regions from 2006 to 2010. Ninety-nine single uredinial isolates were tested for virulence phenotype with 20 Thatcher near-isogenic lines of wheat. Forty-one virulence phenotypes were found in the four regions, with eight in common between the widely separated Central and West Siberia regions. A total of 72 isolates were tested for molecular genotype with 23 simple sequence repeat (SSR) primer pairs, and 66 isolates were used for further analysis after clone correction for virulence and molecular genotype. Analysis of variation showed no overall differentiation of SSR genotypes or virulence phenotypes based on region of origin. Linkage disequilibria for SSR genotypes were high across the entire population. The regional populations had higher than expected levels of allelic heterozygosity that indicated clonal reproduction. Based on cluster analysis of SSR genotypes there were two groups of P.triticina isolates that were widely distributed across Russia. The two SSR groups also differed significantly for virulence. Puccinia triticina may be dispersed from a common source of inoculum in the European or Caucasus regions of Russia. The Russian P.triticina populations were highly differentiated for SSR genotype from populations in Tajikistan, Kyrgyzstan, Uzbekistan, Armenia, Georgia and Azerbaijan and more similar to populations from southern Kazakhstan and northern Kazakhstan. C1 [Kolmer, J. A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Kabdulova, M. G.; Mustafina, M. A.; Zhemchuzhina, N. S.; Dubovoy, V.] Russian Agr Acad, All Russian Res Inst Phytopathol, Moscow 143050, Russia. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM jkolmer@umn.edu FU International Science and Technology Centre FX The authors thank Mark Hughes, Kun Xiao and Amy Fox for excellent technical assistance, and the International Science and Technology Centre for funding M. G. K. NR 21 TC 1 Z9 1 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD APR PY 2015 VL 64 IS 2 BP 328 EP 336 DI 10.1111/ppa.12248 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CD5PC UT WOS:000351139300011 ER PT J AU Yang, CH Odvody, GN Fernandez, CJ Landivar, JA Minzenmayer, RR Nichols, RL AF Yang, Chenghai Odvody, Gary N. Fernandez, Carlos J. Landivar, Juan A. Minzenmayer, Richard R. Nichols, Robert L. TI Evaluating unsupervised and supervised image classification methods for mapping cotton root rot SO PRECISION AGRICULTURE LA English DT Article DE Cotton root rot; Airborne multispectral imagery; Image classification; Vegetation index; Agreement index ID SYSTEM AB Cotton root rot, caused by the soilborne fungus Phymatotrichopsis omnivora, is one of the most destructive plant diseases occurring throughout the southwestern United States. This disease has plagued the cotton industry for over a century, but effective practices for its control are still lacking. Recent research has shown that a commercial fungicide, flutriafol, has potential for the control of cotton root rot. To effectively and economically control this disease, it is necessary to identify infected areas within fields so that site-specific technology can be used to apply fungicide only to the infected areas. The objectives of this study were to evaluate unsupervised classification applied to multispectral imagery, unsupervised classification applied to the normalized difference vegetation index (NDVI)and six supervised classification techniques, including minimum distance, Mahalanobis distance, maximum likelihood and spectral angle mapper (SAM), neural net and support vector machine (SVM),for mapping cotton root rot from airborne multispectral imagery. Two cotton fields with a history of root rot infection in Texas, USA were selected for this study. Airborne imagery with blue, green, red and near-infrared bands was taken from the fields shortly before harvest when infected areas were fully expressed in 2011. The four-band images were classified into infected and non-infected zones using the eight classification methods. Classification agreement index values for infected area estimation between any two methods ranged from 0.90 to 1.00 for both fields, indicating a high degree of agreement among the eight methods. Accuracy assessment showed that all eight methods accurately identified root rot-infected areas with overall accuracy values from 94.0 to 96.5 % for Field 1 and 93.0 to 95.0 % for Field 2. All eight methods appear to be equally effective and accurate for detection of cotton root rot for site-specific management of this disease, though the NDVI-based classification, minimum distance and SAM can be easily implemented without the need for complex image processing capability. These methods can be used by cotton producers and crop consultants to develop prescription maps for effective and economical control of cotton root rot. C1 [Yang, Chenghai] ARS, USDA, Aerial Applicat Technol Res Unit, College Stn, TX 77845 USA. [Odvody, Gary N.; Fernandez, Carlos J.; Landivar, Juan A.] Texas AgriLife Res & Extens Ctr, Corpus Christi, TX 78406 USA. [Minzenmayer, Richard R.] Texas AgriLife Extens Serv, Ballinger, TX 76821 USA. [Nichols, Robert L.] Cotton Inc, Cary, NC 27513 USA. RP Yang, CH (reprint author), ARS, USDA, Aerial Applicat Technol Res Unit, 3103 F & B Rd, College Stn, TX 77845 USA. EM chenghai.yang@ars.usda.gov FU Texas State Support Committee; Cotton Incorporated, Cary, North Carolina FX This project was partly funded by Texas State Support Committee and Cotton Incorporated, Cary, North Carolina. The authors wish to thank Adam Garcia of Edinburg, Texas and Fred Gomez of USDA-ARS at College Station, Texas for taking the airborne imagery for this study and Jim Forward of U.S. Fish and Wildlife Service at Alamo, Texas for assistance in image registration and ground verification. NR 18 TC 3 Z9 3 U1 2 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD APR PY 2015 VL 16 IS 2 BP 201 EP 215 DI 10.1007/s11119-014-9370-9 PG 15 WC Agriculture, Multidisciplinary SC Agriculture GA CD3QZ UT WOS:000350996100006 ER PT J AU Schutze, MK Aketarawong, N Amornsak, W Armstrong, KF Augustinos, AA Barr, N Bo, W Bourtzis, K Boykin, LM Caceres, C Cameron, SL Chapman, TA Chinvinijkul, S Chomic, A De Meyer, M Drosopoulou, E Englezou, A Ekesi, S Gariou-Papalexiou, A Geib, SM Hailstones, D Hasanuzzaman, M Haymer, D Hee, AKW Hendrichs, J Jessup, A Ji, QG Khamis, FM Krosch, MN Leblanc, L Mahmood, K Malacrida, AR Mavragani-Tsipidou, P Mwatawala, M Nishida, R Ono, H Reyes, J Rubinoff, D San Jose, M Shelly, TE Srikachar, S Tan, KH Thanaphum, S Haq, I Vijaysegaran, S Wee, SL Yesmin, F Zacharopoulou, A Clarke, AR AF Schutze, Mark K. Aketarawong, Nidchaya Amornsak, Weerawan Armstrong, Karen F. Augustinos, Antonis A. Barr, Norman Bo, Wang Bourtzis, Kostas Boykin, Laura M. Caceres, Carlos Cameron, Stephen L. Chapman, Toni A. Chinvinijkul, Suksom Chomic, Anastasija De Meyer, Marc Drosopoulou, Ellena Englezou, Anna Ekesi, Sunday Gariou-Papalexiou, Angeliki Geib, Scott M. Hailstones, Deborah Hasanuzzaman, Mohammed Haymer, David Hee, Alvin K. W. Hendrichs, Jorge Jessup, Andrew Ji, Qinge Khamis, Fathiya M. Krosch, Matthew N. Leblanc, Luc Mahmood, Khalid Malacrida, Anna R. Mavragani-Tsipidou, Pinelopi Mwatawala, Maulid Nishida, Ritsuo Ono, Hajime Reyes, Jesus Rubinoff, Daniel San Jose, Michael Shelly, Todd E. Srikachar, Sunyanee Tan, Keng H. Thanaphum, Sujinda Haq, Ihsan Vijaysegaran, Shanmugam Wee, Suk L. Yesmin, Farzana Zacharopoulou, Antigone Clarke, Anthony R. TI Synonymization of key pest species within the Bactrocera dorsalis species complex (Diptera: Tephritidae): taxonomic changes based on a review of 20 years of integrative morphological, molecular, cytogenetic, behavioural and chemoecological data SO SYSTEMATIC ENTOMOLOGY LA English DT Article ID ORIENTAL FRUIT-FLY; FLIES DIPTERA; PHILIPPINENSIS DIPTERA; AEDEAGAL LENGTH; METHYL EUGENOL; WING-SHAPE; MATING COMPATIBILITY; POPULATION-STRUCTURE; CARAMBOLAE DIPTERA; MITOCHONDRIAL-DNA AB Bactrocera papayaeDrew & Hancock, Bactrocera philippinensisDrew & Hancock, Bactrocera carambolaeDrew & Hancock, and Bactrocera invadensDrew, Tsuruta & White are four horticultural pest tephritid fruit fly species that are highly similar, morphologically and genetically, to the destructive pest, the Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). This similarity has rendered the discovery of reliable diagnostic characters problematic, which, in view of the economic importance of these taxa and the international trade implications, has resulted in ongoing difficulties for many areas of plant protection and food security. Consequently, a major international collaborative and integrated multidisciplinary research effort was initiated in 2009 to build upon existing literature with the specific aim of resolving biological species limits among B. papayae, B. philippinensis, B. carambolae, B.invadens and B.dorsalis to overcome constraints to pest management and international trade. Bactrocera philippinensis has recently been synonymized with B. papayae as a result of this initiative and this review corroborates that finding; however, the other names remain in use. While consistent characters have been found to reliably distinguish B. carambolae from B. dorsalis, B. invadens and B. papayae, no such characters have been found to differentiate the latter three putative species. We conclude that B. carambolae is a valid species and that the remaining taxa, B. dorsalis, B. invadens and B. papayae, represent the same species. Thus, we consider B. dorsalis (Hendel) as the senior synonym of B.papayaeDrew and Hancock syn.n. and B. invadensDrew, Tsuruta & White syn.n. A redescription of B. dorsalis is provided. Given the agricultural importance of B. dorsalis, this taxonomic decision will have significant global plant biosecurity implications, affecting pest management, quarantine, international trade, postharvest treatment and basic research. Throughout the paper, we emphasize the value of independent and multidisciplinary tools in delimiting species, particularly in complicated cases involving morphologically cryptic taxa. C1 [Schutze, Mark K.; Cameron, Stephen L.; Vijaysegaran, Shanmugam; Clarke, Anthony R.] Queensland Univ Technol, Sch Earth Environm & Biol Sci, Brisbane, Qld 4001, Australia. [Schutze, Mark K.; Chapman, Toni A.; Englezou, Anna; Hailstones, Deborah; Krosch, Matthew N.; Clarke, Anthony R.] Plant Biosecur Cooperat Res Ctr, Canberra, ACT, Australia. [Aketarawong, Nidchaya] Mahidol Univ, Fac Sci, Dept Biotechnol, Bangkok 10400, Thailand. [Amornsak, Weerawan] Kasetsart Univ, Dept Entomol, Fac Agr, Bangkok, Thailand. [Armstrong, Karen F.; Chomic, Anastasija] Lincoln Univ, Bioprotect Res Ctr, Christchurch, New Zealand. [Augustinos, Antonis A.; Bo, Wang; Bourtzis, Kostas; Caceres, Carlos; Hendrichs, Jorge; Reyes, Jesus; Haq, Ihsan] FAO IAEA Div Nucl Tech Food & Agr, Vienna, Austria. [Augustinos, Antonis A.; Bo, Wang; Bourtzis, Kostas; Caceres, Carlos; Hendrichs, Jorge; Reyes, Jesus; Haq, Ihsan] FAO IAEA Div Nucl Tech Food & Agr, Seibersdorf, Austria. [Augustinos, Antonis A.] Univ Patras, Dept Environm & Nat Resources Management, Agrinion, Greece. [Barr, Norman] USDA APHIS, Ctr Plant Hlth Sci & Technol, Mission Lab, Edinburg, TX USA. [Bo, Wang; Ji, Qinge] Fujian Agr & Forestry Univ, Fuzhou, Peoples R China. [Boykin, Laura M.] Univ Western Australia, ARC Ctr Excellence Plant Energy Biol, Perth, WA 6009, Australia. [Boykin, Laura M.] Univ Western Australia, Sch Chem & Biochem, Perth, WA 6009, Australia. [Chapman, Toni A.; Englezou, Anna; Hailstones, Deborah] NSW Dept Primary Ind, Elizabeth Macarthur Agr Inst, Menangle, Australia. [Chinvinijkul, Suksom] Minist Agr & Cooperat, Dept Agr Extens, Bangkok, Thailand. [De Meyer, Marc] Royal Museum Cent Africa, Invertebrates Sect, Tervuren, Belgium. [Drosopoulou, Ellena; Mavragani-Tsipidou, Pinelopi] Aristotle Univ Thessaloniki, Fac Sci, Sch Biol, Dept Genet Dev & Mol Biol, GR-54006 Thessaloniki, Greece. [Ekesi, Sunday; Khamis, Fathiya M.] ICIPE, Nairobi, Kenya. [Gariou-Papalexiou, Angeliki; Zacharopoulou, Antigone] Univ Patras, Dept Biol, Patras, Greece. [Geib, Scott M.] ARS, Trop Crop & Commod Protect Res Unit, Pacific Basin Agr Res Ctr, USDA, Hilo, HI USA. [Hasanuzzaman, Mohammed; Yesmin, Farzana] Bangladesh Atom Energy Commiss, Inst Food & Radiat Biol, Atom Energy Res Estab, Dhaka, Bangladesh. [Haymer, David] Univ Hawaii, Dept Cell & Mol Biol, Honolulu, HI 96822 USA. [Hee, Alvin K. W.] Univ Putra Malaysia, Fac Sci, Dept Biol, Serdang 43400, Malaysia. [Jessup, Andrew] NSW Dept Primary Ind, Cent Coast Primary Ind Ctr, Gosford, Australia. [Krosch, Matthew N.] Univ Queensland, Sustainable Minerals Inst, Ctr Water Minerals Ind, St Lucia, Qld, Australia. [Leblanc, Luc; Rubinoff, Daniel; San Jose, Michael] Univ Hawaii Manoa, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA. [Mahmood, Khalid] Pakistan Museum Nat Hist, Islamabad, Pakistan. [Malacrida, Anna R.] Univ Pavia, Dept Biol & Biotechnol, I-27100 Pavia, Italy. [Mwatawala, Maulid] Sokoine Univ Agr, Dept Crop Sci & Prod, Morogoro, Tanzania. [Nishida, Ritsuo; Ono, Hajime] Kyoto Univ, Grad Sch Agr, Kyoto, Japan. [Shelly, Todd E.] USDA APHIS, Honolulu, HI USA. [Srikachar, Sunyanee] Minist Agr & Cooperat, Dept Agr, Bangkok, Thailand. [Tan, Keng H.] Tan Hak Heng, George Town, Malaysia. [Thanaphum, Sujinda] Mahidol Univ, Fac Sci, Dept Biotechnol, Fruit Fly Mol Genet Lab, Bangkok 10400, Thailand. [Haq, Ihsan] Natl Agr Res Ctr, Islamabad, Pakistan. [Wee, Suk L.] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Environm & Nat Resource Sci, Bangi 43600, Malaysia. RP Schutze, MK (reprint author), Queensland Univ Technol, Sch Earth Environm & Biol Sci, GPO Box 2434, Brisbane, Qld 4001, Australia. EM m.schutze@qut.edu.au RI Cameron, Stephen/A-3742-2008; Jessup, Andrew/B-2510-2016; OI Cameron, Stephen/0000-0002-6694-4130; ONO, HAJIME/0000-0002-8042-2697; Boykin, Laura/0000-0002-6101-1921; Jessup, Andrew/0000-0003-2318-7835; , Ihsan/0000-0001-5879-7698; Jessup, Andrew/0000-0002-0479-7489; Chapman, Toni/0000-0002-4571-1629; Clarke, Anthony/0000-0002-8165-3032; Hailstones, Deborah/0000-0003-3042-5168 FU CRC National Plant Biosecurity and Plant Biosecurity CRC projects [CRC20115, CRC20183, PBCRC3024, PBCRC3066]; Australian Government's Cooperative Research Centres Program; Australian Research Council [FT120100746]; United States Department of Agriculture through Farm Bill funding [3.0251]; Tertiary Education Commission of New Zealand; UN-FAO/IAEA via the Coordinated Research Project 'Resolution of cryptic species complexes of tephritid pests to overcome constraints to SIT application and international trade' FX M.K.S., A.R.C., K.F.A., T.A.C., A.C., A.E., D.H. and M.N.K. were supported entirely or in part through CRC National Plant Biosecurity and Plant Biosecurity CRC projects CRC20115, CRC20183, PBCRC3024 and PBCRC3066. These authors acknowledge the support of the Australian Government's Cooperative Research Centres Program. S.L.C. was supported by an Australian Research Council Future Fellowship (FT120100746). L.L. and M.S.J. were supported through a Cooperative Agreement from the United States Department of Agriculture through Farm Bill funding (project 3.0251) administered by the College of Tropical Agriculture and Human Resources of the University of Hawaii. K.F.A. and L.M.B. were supported by the Tertiary Education Commission of New Zealand. The authors sincerely acknowledge support provided by the UN-FAO/IAEA via the Coordinated Research Project 'Resolution of cryptic species complexes of tephritid pests to overcome constraints to SIT application and international trade'. We thank Bruce Halliday of the CSIRO for nomenclatural advice. The authors thank the numerous anonymous reviewers for improving the manuscript. NR 91 TC 37 Z9 41 U1 8 U2 62 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6970 EI 1365-3113 J9 SYST ENTOMOL JI Syst. Entomol. PD APR PY 2015 VL 40 IS 2 BP 456 EP 471 DI 10.1111/syen.12113 PG 16 WC Evolutionary Biology; Entomology SC Evolutionary Biology; Entomology GA CD3ML UT WOS:000350982700010 ER PT J AU Knetter, SM Bearson, SMD Huang, TH Kurkiewicz, D Schroyen, M Nettleton, D Berman, D Cohen, V Lunney, JK Ramer-Tait, AE Wannemuehler, MJ Tuggle, CK AF Knetter, Susan M. Bearson, Shawn M. D. Huang, Ting-Hua Kurkiewicz, Dason Schroyen, Martine Nettleton, Dan Berman, Daniel Cohen, Valerie Lunney, Joan K. Ramer-Tait, Amanda E. Wannemuehler, Michael J. Tuggle, Christopher K. TI Salmonella enterica serovar Typhimurium-infected pigs with different shedding levels exhibit distinct clinical, peripheral cytokine and transcriptomic immune response phenotypes SO INNATE IMMUNITY LA English DT Article DE Cytokine; immune response; Salmonella; swine; transcriptomics ID NECROSIS-FACTOR-ALPHA; IFN-GAMMA; SEROTYPE TYPHIMURIUM; PRRSV VACCINATION; DENDRITIC CELLS; T-CELLS; SWINE; EXPRESSION; HOST; INFLAMMATION AB Foodborne salmonellosis costs the US $2.7billion/year, including $100.0million in annual losses to pork producers. Pigs colonized with Salmonella are usually asymptomatic with varied severity and duration of fecal shedding. Thus, understanding the responses that result in less shedding may provide a mechanism for control. Fifty-four pigs were inoculated with Salmonella enterica serovar Typhimurium (ST) and clinical signs, fecal ST shedding, growth performance, peripheral cytokines and whole blood gene expression were measured. Persistently shedding (PS) pigs had longer pyrexia and elevated serum IL-1, TNF- and IFN- compared with low shedding (LS) pigs, while LS pigs had brief pyrexia, less shedding that decreased more rapidly and greater serum CXCL8 than PS pigs. The PS pigs up-regulated genes involved with the STAT1, IFNB1 and IFN- networks on d 2, while up-regulation of genes involved in immune response regulation were only detected in LS pigs. This is the first study to examine host responses to ST infection at a clinical, performance, cytokine and transcriptomic level. The results indicated that pigs with different shedding outcomes developed distinct immune responses within the first 2d of ST infection, and elucidated alternative mechanisms that could be targeted to reduce Salmonella shedding and spread. C1 [Knetter, Susan M.; Huang, Ting-Hua; Schroyen, Martine; Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Bearson, Shawn M. D.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Kurkiewicz, Dason; Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Berman, Daniel; Cohen, Valerie; Lunney, Joan K.] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD USA. [Ramer-Tait, Amanda E.] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. [Wannemuehler, Michael J.] Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. RP Tuggle, CK (reprint author), Iowa State Univ, Dept Anim Sci, 2255 Kildee Hall, Ames, IA 50011 USA. EM cktuggle@iastate.edu FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2009-35205-05192]; USDA-ARS-NADC; USDA-ARS-BARC FX This work was supported by a National Research Initiative Competitive Grant [no. 2009-35205-05192] from the USDA National Institute of Food and Agriculture, the USDA-ARS-NADC and the USDA-ARS-BARC. NR 60 TC 7 Z9 7 U1 2 U2 10 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1753-4259 EI 1753-4267 J9 INNATE IMMUN-LONDON JI Innate Immun. PD APR PY 2015 VL 21 IS 3 BP 227 EP 241 DI 10.1177/1753425914525812 PG 15 WC Biochemistry & Molecular Biology; Immunology; Medicine, Research & Experimental; Microbiology SC Biochemistry & Molecular Biology; Immunology; Research & Experimental Medicine; Microbiology GA CC3IQ UT WOS:000350240900001 PM 24632525 ER PT J AU Low, SA Isserman, AM AF Low, Sarah A. Isserman, Andrew M. TI Where Are the Innovative Entrepreneurs? Identifying Innovative Industries and Measuring Innovative Entrepreneurship SO INTERNATIONAL REGIONAL SCIENCE REVIEW LA English DT Article DE entrepreneurship; self-employment; establishment birthrate; innovative industries; regional entrepreneurship; defining and measuring entrepreneurship ID CREATIVE CLASS; GROWTH; US; EMPLOYMENT; STATES AB This article concentrates on a crucial technical aspect of regional entrepreneurship research: how do we measure the most innovative of entrepreneurs, the entrepreneurs most likely to create regional growth? Innovation is a crucial component of entrepreneurship; yet, the frequent use of entrepreneurship proxies that do not consider innovation motivated us to propose and develop an indicator of innovative entrepreneurship that is useful for studies of regions, counties, states, and metropolitan areas as well. We posit that a novel combination of start-ups in innovative industries and self-employed in innovative industries yields entrepreneurship indicators that incorporate three widely recognized functions of entrepreneurship, including innovation. We detect sharp contrasts between our innovative entrepreneurship indicators and widely used entrepreneurship proxies. Our analysis demonstrates that innovative entrepreneurship is a useful empirical concept and that ignoring innovation in entrepreneurship likely has produced misleading research results and policy implications about regional entrepreneurship, its determinants, and its role in regional economic growth. C1 [Low, Sarah A.] Econ Res Serv, USDA, Washington, DC 20250 USA. [Isserman, Andrew M.] Univ Illinois, Dept Agr & Consumer Econ & Urban & Reg Planning, Champaign, IL USA. RP Low, SA (reprint author), Econ Res Serv, USDA, 1400 Independence Ave SW,MS 1800, Washington, DC 20250 USA. EM slow@ers.usda.gov NR 56 TC 3 Z9 3 U1 4 U2 37 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0160-0176 EI 1552-6925 J9 INT REGIONAL SCI REV JI Int. Reg. Sci. Rev. PD APR PY 2015 VL 38 IS 2 BP 171 EP 201 DI 10.1177/0160017613484926 PG 31 WC Environmental Studies; Planning & Development; Urban Studies SC Environmental Sciences & Ecology; Public Administration; Urban Studies GA CC6MA UT WOS:000350477900003 ER PT J AU Mohammed, AR Cothren, JT Chen, MH Tarpley, L AF Mohammed, A. R. Cothren, J. T. Chen, M. -H. Tarpley, L. TI 1-Methylcyclopropene (1-MCP)-Induced Alteration in Leaf Photosynthetic Rate, Chlorophyll Fluorescence, Respiration and Membrane Damage in Rice (Oryza sativa L.) Under High Night Temperature SO JOURNAL OF AGRONOMY AND CROP SCIENCE LA English DT Article DE 1-methylcyclopropene; chlorophyll fluorescence; high night temperature; photosynthesis; respiration; rice ID ELEVATED CO2; ETHYLENE PRODUCTION; MILLING QUALITY; MALE-STERILITY; YIELD; PLANTS; NITROGEN; STRESS; POLLEN; ACCUMULATION AB High night temperature (HNT) can induce ethylene-triggered reactive oxygen species production, which can cause premature leaf senescence and membrane damage, thereby affecting production, consumption and transfer of photosyn-thates, and yield. The 1-methylcyclopropene (1-MCP) can competitively bind with ethylene receptors and decrease ethylene effects. The objective was to determine the effects of HNT and 1-MCP on leaf photosynthetic rate (P-N), chlorophyll fluorescence, total chlorophyll (TC), respiration, membrane damage, pollen germination, spikelet fertility (SF) and yield of rice hybrid XL723'. Plants were grown under ambient night temperature (ANT) (25 degrees C) or HNT (30 degrees C) with or without 1-MCP treatment. Application of 1-MCP was at the boot stage. The decrease in yield (11%) under HNT was associated with decreased P-N (4%), stomatal conductance (8%), quantum yield (11%) TC (23%) and SF (5%) and increased respiration (74%), F-o/F-m (increase in thylakoid membrane damage; 11%) and membrane damage (leaf electrolytic leakage; 57%). The 1-MCP-treated plants grown under HNT showed increased yield (17%), which was associated with increased P-N (10%), stomatal conductance (30%), quantum yield (9%), TC (37%) and SF (11%) and decreased respiration (39%), F-o/F-m (5%) and membrane damage (18%). Plants grown under HNT showed increased grain chalkiness (154%) compared with plants grown under ANT. C1 [Mohammed, A. R.; Tarpley, L.] Texas A&M AgriLife Res Ctr, Beaumont, TX 77713 USA. [Cothren, J. T.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Chen, M. -H.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. RP Mohammed, AR (reprint author), Texas A&M AgriLife Res Ctr, 1509 Aggie Dr, Beaumont, TX 77713 USA. EM abdulrazack@tamu.edu FU AgroFresh Inc. FX The authors thank AgroFresh Inc. for their financial support. We would also like to thank Mr. James Medley for his help in the leaf nitrogen analysis and Ms. Huma F. Mohammed for her help in measuring leaf respiration. NR 60 TC 0 Z9 0 U1 5 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2250 EI 1439-037X J9 J AGRON CROP SCI JI J. Agron. Crop Sci. PD APR PY 2015 VL 201 IS 2 BP 105 EP 116 DI 10.1111/jac.12096 PG 12 WC Agronomy SC Agriculture GA CC7JR UT WOS:000350544000003 ER PT J AU Iverson, LR Collins, S Dangermond, J Forman, R Nassauer, JI Wiens, J Wolfe, E AF Iverson, Louis R. Collins, Scott Dangermond, Jack Forman, Richard Nassauer, Joan I. Wiens, John Wolfe, Erin TI In memoriam: Paul G. Risser (1939-2014) SO LANDSCAPE ECOLOGY LA English DT Biographical-Item C1 [Iverson, Louis R.] US Forest Serv, USDA, Delaware, OH 43015 USA. [Collins, Scott] Univ New Mexico, Albuquerque, NM 87131 USA. [Dangermond, Jack] ESRI, Redlands, CA USA. [Forman, Richard] Harvard Univ, Cambridge, MA 02138 USA. [Nassauer, Joan I.] Univ Michigan, Ann Arbor, MI 48109 USA. [Wiens, John] Oregon State Univ, Corvallis, OR 97330 USA. [Wolfe, Erin] Univ Oklahoma, Norman, OK 73072 USA. RP Iverson, LR (reprint author), US Forest Serv, USDA, Delaware, OH 43015 USA. EM liverson@fs.fed.us; scollins@sevilleta.unm.edu; jdangermond@esri.com; rforman@gsd.harvard.edu; nassauer@umich.edu; jwiens300@gmail.com; ewolfe@ou.edu NR 1 TC 0 Z9 0 U1 1 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD APR PY 2015 VL 30 IS 4 BP 579 EP 583 DI 10.1007/s10980-015-0174-8 PG 5 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CC4XT UT WOS:000350360100001 ER PT J AU Kusano, M Baxter, I Fukushima, A Oikawa, A Okazaki, Y Nakabayashi, R Bouvrette, DJ Achard, F Jakubowski, AR Ballam, JM Phillips, JR Culler, AH Saito, K Harrigan, GG AF Kusano, Miyako Baxter, Ivan Fukushima, Atsushi Oikawa, Akira Okazaki, Yozo Nakabayashi, Ryo Bouvrette, Denise J. Achard, Frederic Jakubowski, Andrew R. Ballam, Joan M. Phillips, Jonathan R. Culler, Angela H. Saito, Kazuki Harrigan, George G. TI Assessing metabolomic and chemical diversity of a soybean lineage representing 35 years of breeding SO METABOLOMICS LA English DT Article DE Soybean (Glycine max) breeding; Food safety; Metabolomics; Ionomics ID GENETIC DIVERSITY; ACCUMULATION; ENVIRONMENT; IMPROVEMENT; GERMPLASM; CROPS; SEEDS; RICE AB Information on crop genotype- and phenotype-metabolite associations can be of value to trait development as well as to food security and safety. The unique study presented here assessed seed metabolomic and ionomic diversity in a soybean lineage representing similar to 35 years of breeding (launch years 1972-2008) and increasing yield potential. Selected varieties included six conventional and three genetically modified (GM) glyphosate-tolerant lines. A metabolomics approach utilizing capillary electrophoresis (CE)-time-of-flight-mass spectrometry (TOF-MS), gas chromatography (GC)-TOF-MS and liquid chromatography (LC)-quadrupole (q)-TOFMS resulted in measurement of a total of 732 annotated peaks. Ionomics through inductively-coupled plasma (ICP)-MS profiled twenty mineral elements. Orthogonal partial least squares-discriminant analysis (OPLS-DA) of the seed data successfully differentiated newer higher-yielding soybean from earlier lower-yielding accessions at both field sites. This result reflected genetic fingerprinting data that demonstrated a similar distinction between the newer and older soybean. Correlation analysis also revealed associations between yield data and specific metabolites. There were no clear metabolic differences between the conventional and GM lines. Overall, observations of metabolic and genetic differences between older and newer soybean varieties provided novel and significant information on the impact of varietal development on biochemical variability. Proposed applications of omics in food and feed safety assessments will need to consider that GM is not a major source of metabolite variability and that trait development in crops will, of necessity, be associated with biochemical variation. C1 [Kusano, Miyako; Fukushima, Atsushi; Oikawa, Akira; Okazaki, Yozo; Nakabayashi, Ryo; Saito, Kazuki] RIKEN Ctr Sustainable Resource Sci, Tsunumi Ku, Yokohama, Kanagawa 23000405, Japan. [Baxter, Ivan] ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, USDA, St Louis, MO 63132 USA. [Bouvrette, Denise J.; Achard, Frederic; Jakubowski, Andrew R.; Ballam, Joan M.; Phillips, Jonathan R.; Culler, Angela H.; Harrigan, George G.] Monsanto Co, St Louis, MO 63167 USA. [Saito, Kazuki] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Chiba 2608675, Japan. RP Baxter, I (reprint author), ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, USDA, St Louis, MO 63132 USA. EM ivan.baxter@ars.usda.gov; kazuki.saito@riken.jp; george.g.harrigan@monsanto.com RI Saito, Kazuki/D-2670-2009; Baxter, Ivan/A-1052-2009; Fukushima, Atsushi/C-9279-2017; Nakabayashi, Ryo/C-9734-2017; Oikawa, Akira/D-2715-2017 OI Saito, Kazuki/0000-0001-6310-5342; Baxter, Ivan/0000-0001-6680-1722; Fukushima, Atsushi/0000-0001-9015-1694; Nakabayashi, Ryo/0000-0002-8674-0928; FU Japan Advanced Plant Science Network FX We are very grateful for the agronomic support provided by Matt Culler of Monsanto. Genetic fingerprint analyses were conducted within the Molecular Breeding Technology group at Monsanto. The excellent logistical support provided by James McCarter and his sample management team was also critical to the success of this experiment. We are indebted to Nordine Cheikh for his support and encouragement and to Mark Leibman and John Vicini for helpful comments on the manuscript. We also thank Tomoko Nishizawa, Makoto Kobayashi, Ryosuke Sasaki, and Koji Takano (RIKEN) for their technical assistance in the study. This research was also supported by the Japan Advanced Plant Science Network. NR 32 TC 7 Z9 7 U1 7 U2 55 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD APR PY 2015 VL 11 IS 2 BP 261 EP 270 DI 10.1007/s11306-014-0702-6 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CC7TC UT WOS:000350570300003 ER PT J AU Woodruff, DR Meinzer, FC Marias, DE Sevanto, S Jenkins, MW McDowell, NG AF Woodruff, David R. Meinzer, Frederick C. Marias, Danielle E. Sevanto, Sanna Jenkins, Michael W. McDowell, Nate G. TI Linking nonstructural carbohydrate dynamics to gas exchange and leaf hydraulic behavior in Pinus edulis and Juniperus monosperma SO NEW PHYTOLOGIST LA English DT Article DE carbon starvation; carbon storage; drought; growth limitation; Juniperus monosperma; leaf hydraulics; nonstructural carbohydrate (NSC); Pinus edulis ID WATER-STRESS; STOMATAL CONDUCTANCE; PHLOEM TRANSPORT; TREE MORTALITY; DROUGHT STRESS; DOUGLAS-FIR; DIE-OFF; CARBON; FOREST; PLANTS AB Leaf hydraulics, gas exchange and carbon storage in Pinus edulis and Juniperus monosperma, two tree species on opposite ends of the isohydry-anisohydry spectrum, were analyzed to examine relationships between hydraulic function and carbohydrate dynamics. Leaf hydraulic vulnerability, leaf water potential ((l)), leaf hydraulic conductance (K-leaf), photosynthesis (A), stomatal conductance (g(s)) and nonstructural carbohydrate (NSC) content were analyzed throughout the growing season. Leaf hydraulic vulnerability was significantly lower in the relatively anisohydric J.monosperma than in the more isohydric P.edulis. In P.edulis, (l) dropped and stayed below 50% loss of leaf hydraulic conductance (P-50) early in the day during May, August and around midday in September, leading to sustained reductions in K-leaf. In J.monosperma, (l) dropped below P-50 only during August, resulting in the maintenance of K-leaf during much of the growing season. Mean A and g(s) during September were significantly lower in P.edulis than in J.monosperma. Foliar total NSC was two to three times greater in J.monosperma than in P.edulis in June, August and September. Consistently lower levels of total NSC in P.edulis suggest that its isohydric strategy pushes it towards the exhaustion of carbon reserves during much of the growing season. C1 [Woodruff, David R.; Meinzer, Frederick C.] US Forest Serv, USDA, PNW Res Stn, Corvallis, OR 97331 USA. [Marias, Danielle E.] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Sevanto, Sanna; McDowell, Nate G.] Los Alamos Natl Lab, Earth & Environm Sci Div, Los Alamos, NM 87545 USA. [Jenkins, Michael W.] Univ Calif Santa Cruz, Dept Environm Studies, Santa Cruz, CA 95064 USA. RP Woodruff, DR (reprint author), US Forest Serv, USDA, PNW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM dwoodruff@fs.fed.us FU Los Alamos National Laboratory [DE-NA0001302]; USDA Forest Service Pacific Northwest Research Station [DE-NA0001302]; Department of Energy, Office of Science FX This work was supported in part by interagency agreement DE-NA0001302 between Los Alamos National Laboratory and the USDA Forest Service Pacific Northwest Research Station, and by the Department of Energy, Office of Science. We are grateful to Kristen Falk for help with laboratory work and to three anonymous reviewers for comments that resulted in an improved paper. NR 54 TC 10 Z9 11 U1 12 U2 73 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD APR PY 2015 VL 206 IS 1 BP 411 EP 421 DI 10.1111/nph.13170 PG 11 WC Plant Sciences SC Plant Sciences GA CC4TT UT WOS:000350347500039 PM 25412472 ER PT J AU Shen, GX Wei, J Qiu, XY Hu, RB Kuppu, S Auld, D Blumwald, E Gaxiola, R Payton, P Zhang, H AF Shen, Guoxin Wei, Jia Qiu, Xiaoyun Hu, Rongbin Kuppu, Sundaram Auld, Dick Blumwald, Eduardo Gaxiola, Roberto Payton, Paxton Zhang, Hong TI Co-overexpression of AVP1 and AtNHX1 in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE AtNHX1; AVP1; Auxin transport; Cotton; Drought tolerance; Salt tolerance ID VACUOLAR NA+/H+ ANTIPORTER; PYROPHOSPHATASE GENE AVP1; INCREASES FIBER YIELD; SALINITY TOLERANCE; PROTON PUMPS; H+-PPASE; ARABIDOPSIS; EXPRESSION; COEXPRESSION; TOMATO AB Salinity and drought are two major environmental stresses that limit the growth and productivity of cotton. To improve cotton's drought and salt tolerance, transgenic cotton plants expressing the Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 and H+-pyrophosphatase gene AVP1 were produced by cross-pollination of two single-gene-overexpressing plants. The salt tolerance and drought tolerance were further enhanced by simultaneously overexpressing AVP1 and AtNHX1 in comparison to AVP1 or AtNHX1 single-gene-overexpressing plants and to wild-type plants. Plant height, boll number, and fiber yield of AVP1/AtNHX1-co-overexpressing plants were higher than those of AVP1-overexpressing, AtNHX1-overexpressing, segregated non-transgenic line, and wild-type plants under saline and drought conditions. The photosynthetic rate of AVP1/AtNHX1-co-overexpressing plants was significantly higher than that of single-gene-overexpressing and wild-type plants under 200 mM NaCl treatment. In addition, the root systems of AVP1/AtNHX1-co-overexpressing plants were larger than those of single-gene-overexpressing and wild-type plants, which was likely due to increased auxin polar transport in the root systems of the AVP1/AtNHX1-co-overexpressing plants. Moreover, these AVP1/AtNHX1-co-overexpressing cotton plants produced 24 % higher fiber yield under low-irrigation conditions and 35 % higher fiber yield under dryland conditions as compared to wild-type cotton in the field. C1 [Shen, Guoxin; Wei, Jia; Qiu, Xiaoyun] Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China. [Hu, Rongbin; Kuppu, Sundaram; Zhang, Hong] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. [Auld, Dick] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. [Blumwald, Eduardo] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Gaxiola, Roberto] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Payton, Paxton] USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. RP Shen, GX (reprint author), Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Zhejiang, Peoples R China. EM guoxinshen@gmail.com; Hong.zhang@ttu.edu RI HU, RONGBIN/B-2225-2017; OI HU, RONGBIN/0000-0001-5921-6891 FU National Natural Science Foundation of China [31170793]; National Natural Science Foundation of Zhejiang province [Z12C130011]; program of Shaoxing 330 Overseas Elites; national research initiative competitive grant from USDA National Institute of Food and Agriculture [2007-35100-18382] FX This work was supported by grants from the National Natural Science Foundation of China to Guoxin Shen (31170793), the National Natural Science Foundation of Zhejiang province to Guoxin Shen (Z12C130011), and the program of Shaoxing 330 Overseas Elites to Guoxin Shen. This work was also supported by a national research initiative competitive grant (no. 2007-35100-18382) from USDA National Institute of Food and Agriculture. NR 29 TC 10 Z9 10 U1 2 U2 49 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD APR PY 2015 VL 33 IS 2 BP 167 EP 177 DI 10.1007/s11105-014-0739-8 PG 11 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CC9CK UT WOS:000350665000001 ER PT J AU Lobo, GP Frankenberger, JR Flanagan, DC Bonilla, CA AF Lobo, Gabriel P. Frankenberger, James R. Flanagan, Dennis C. Bonilla, Carlos A. TI Evaluation and improvement of the CLIGEN model for storm and rainfall erosivity generation in Central Chile SO CATENA LA English DT Article DE CLIGEN; Rainfall erosivity; Storm generation; Water erosion; Weather generator; WEPP ID EROSION PREDICTION; PRECIPITATION DATA; LOESS PLATEAU; VALIDATION; PARAMETERS; RUNOFF; CHINA AB CLIGEN (Climate GENerator) is a stochastic weather generator that produces daily estimates of precipitation and individual storm parameters, including time to peak, peak intensity and storm duration. These parameters are typically used as inputs for other models, such as the Water Erosion Prediction Project (WEPP) model. Although CLIGEN has been proven to be effective for predicting daily estimates, some discrepancies have been observed when generating storm parameters, such as the storm duration. Therefore, a study was conducted to evaluate and improve CLIGEN for storm generation. Individual rainfall events were identified from 1-h pluviograph records that were collected from 30 sites in Central Chile. In this study, 415 years of data were used; 18,012 storms were analyzed. In addition, rainfall erosivity was computed for all storms using the prescribed method to compare the energy provided by the measured and generated rainfall events. Using measured rainfall data, a procedure was developed to improve the CLIGEN estimates by calibrating the input parameter that controls the storm durations. This procedure in turn improved the rainfall intensities and erosivities. The model was tested before and after calibration with the measured rainfall data from the 30 sites in both the wet and dry seasons. Based on a monthly rainfall analysis, the results demonstrated that the number of storms and rainfall amounts, which are not affected by the calibration process, were accurately estimated with CLIGEN. However, before the calibration, especially in the wet season, the storm durations and maximum, intensities were consistently overestimated and underestimated, respectively, at most of the sites and for most months. Therefore, the annual rainfall erosivities were underestimated with CLIGEN at 19 of the 30 sites. After performing the calibration, the R-2 value for the CLIGEN-generated storm durations increased from 0.41 to 0.65. The maximum intensities also exhibited an improvement; the R-2 value increased from 031 to 0.60. Consequently, annual rainfall erosivities were generated with an R-2 value of 0.89; these erosivities were accurately estimated at 29 of the 30 sites. Therefore, this calibration procedure proved to be an effective alternative for generating more reliable storm patterns. This paper explains the procedure in detail and analyzes the parameters related to the individual storm generation process. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lobo, Gabriel P.; Bonilla, Carlos A.] Pontificia Univ Catolica Chile, Dept Ingn Hidraul & Ambiental, Santiago, Chile. [Frankenberger, James R.; Flanagan, Dennis C.] Purdue Univ, USDA ARS Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Bonilla, Carlos A.] Ctr Desarrollo Urbana Sustentable CEDEUS, Santiago, Chile. RP Bonilla, CA (reprint author), Pontificia Univ Catolica Chile, Dept Ingn Hidraul & Ambiental, Ave Vicuna Mackenna 4860, Santiago, Chile. EM cbonilla@ing.puc.cl RI Bonilla, Carlos/A-1676-2013 FU National Commission for Scientific and Technological Research CONICYT/FONDECYT/Regular [1130928] FX This research was supported by funding from the National Commission for Scientific and Technological Research CONICYT/FONDECYT/Regular 1130928. The rainfall data were provided by the General Directorate of Water Resources (DGA), Government of Chile. NR 24 TC 3 Z9 4 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD APR PY 2015 VL 127 BP 206 EP 213 DI 10.1016/j.catena.2015.01.002 PG 8 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA CB8KF UT WOS:000349877800022 ER PT J AU Fishman, ML Chau, HK Qi, PX Hotchkiss, AT Garcia, RA Cooke, PH AF Fishman, Marshall L. Chau, Hoa K. Qi, Phoebe X. Hotchkiss, Arland T. Garcia, Rafael A. Cooke, Peter H. TI Characterization of the global structure of low methoxyl pectin in solution SO FOOD HYDROCOLLOIDS LA English DT Article DE Low methoxyl citrus pectin; High performance size exclusion chromatography; Atomic force microscopy; Pectin structure ID ATOMIC-FORCE MICROSCOPY; SUGAR-BEET PECTIN; MOLECULAR-WEIGHT; GELS; MACROMOLECULES; COMPONENTS; TOMATO AB Low methoxyl citrus pectin (LMP) and amidated low methoxyl pectin (LMAP) were characterized by high performance size exclusion chromatography (HPSEC) with online light scattering (LS), intrinsic viscosity (eta(w)), differential refractive index (dRI) and ultra-violet (UV) detection, by amino acid analysis (AAA) and by atomic force microscopy (AFM). HPSEC revealed the following: the weight average molar mass (M-w), ranged 103-288 x 10(3) Da, (eta(w)), ranged 2.69-4.27 dL/g, radius of gyration (Rg(z)), was 28.6-49.5 nm and hydrated radius (Rh-zv), was 26.3-41.8 nm. The presence of phenylalanine and tyrosine residues as shown by the UV absorbance at 278 nm in the high molar mass range is indicative of protein or fragments associated with LMP derived from citrus fruit. The rho value (Rg(z)/Rh-zv) as a function of molar mass indicated that high molar mass pectin was more compact in shape than intermediate molar mass pectin. AFM images in this study and earlier studies indicate that high methoxyl pectin (HMP) and LMP form network structures in aqueous solution. Unlike HMP networks, LMP networks do not dissociate when dissolved in water at concentrations that are less than 6.6 mu g/mL. Furthermore, AFM images, M-w and eta(w) values for LMP and LMAP indicate that little or no added sugar is bound. Published by Elsevier Ltd. C1 [Fishman, Marshall L.; Chau, Hoa K.; Qi, Phoebe X.; Hotchkiss, Arland T.] USDA ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Garcia, Rafael A.] USDA ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Cooke, Peter H.] New Mexico State Univ, Core Univ, Res Resources Lab, Las Cruces, NM 88003 USA. RP Fishman, ML (reprint author), USDA ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM marshall.fishman@ars.usda.gov FU National Science [MRI-DMR 1229558] FX We thank Andre White for technical assistance in determining carbohydrate compositional analysis. We thank Robyn Moten and Michael Kurantz for technical assistance in determining crude protein content. This material is based in part upon work supported by the National Science under Grant Numbers MRI-DMR 1229558. NR 27 TC 9 Z9 9 U1 11 U2 50 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD APR PY 2015 VL 46 BP 153 EP 159 DI 10.1016/j.foodhyd.2014.12.021 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CB6RD UT WOS:000349753400017 ER PT J AU Colliander, A Jackson, T McNairn, H Chazanoff, S Dinardo, S Latham, B O'Dwyer, I Chun, W Yueh, S Njoku, E AF Colliander, Andreas Jackson, Thomas McNairn, Heather Chazanoff, Seth Dinardo, Steve Latham, Barron O'Dwyer, Ian Chun, William Yueh, Simon Njoku, Eni TI Comparison of Airborne Passive and Active L-Band System (PALS) Brightness Temperature Measurements to SMOS Observations During the SMAP Validation Experiment 2012 (SMAPVEX12) SO IEEE GEOSCIENCE AND REMOTE SENSING LETTERS LA English DT Article DE Passive Active L-band System (PALS); SMAP Validation Experiment 2012 (SMAPVEX12); Soil moisture; Soil Moisture Active Passive (SMAP); Soil Moisture and Ocean Salinity (SMOS) ID SOIL-MOISTURE; OCEAN SALINITY; SENSOR; RETRIEVAL; ALGORITHM AB In this letter, it is shown that spaceborne observations made by the European Space Agency's Soil Moisture and Ocean Salinity (SMOS) satellite agreed closely with the Passive Active L-band System (PALS) brightness temperature acquisitions during the Soil Moisture Active Passive (SMAP) Validation Experiment 2012. The difference between the SMOS and PALS measurements was less than 5 K and 6 K for vertical and horizontal polarizations, respectively, over the relatively homogeneous agricultural areas. These values are less than the SMOS subpixel variability determined from the PALS measurement. This result demonstrated that the measurements obtained in the experiment are scalable to spaceborne brightness temperature observations, are representative of the expected SMAP observations, and will be of value in the development of soil moisture algorithms for spaceborne missions. C1 [Colliander, Andreas; Chazanoff, Seth; Dinardo, Steve; Latham, Barron; O'Dwyer, Ian; Chun, William; Yueh, Simon; Njoku, Eni] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. [Jackson, Thomas] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [McNairn, Heather] Agr & Agri Food Canada, Sci & Technol Branch, Ottawa, ON K1A 0C6, Canada. RP Colliander, A (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. NR 18 TC 7 Z9 7 U1 4 U2 18 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1545-598X EI 1558-0571 J9 IEEE GEOSCI REMOTE S JI IEEE Geosci. Remote Sens. Lett. PD APR PY 2015 VL 12 IS 4 BP 801 EP 805 DI 10.1109/LGRS.2014.2362889 PG 5 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA CB5NM UT WOS:000349674400023 ER PT J AU Tussing-Humphreys, LM Thomson, JL Onufrak, SJ AF Tussing-Humphreys, Lisa M. Thomson, Jessica L. Onufrak, Stephen J. TI A Church-Based Pilot Study Designed to Improve Dietary Quality for Rural, Lower Mississippi Delta, African American Adults SO JOURNAL OF RELIGION & HEALTH LA English DT Article DE Diet; Mississippi; African Americans; Adult; Church ID WEIGHT-LOSS INTERVENTION; FOOD-INTAKE; PHYSICAL-ACTIVITY; HEALTH-PROMOTION; LOSS MAINTENANCE; LOSS PROGRAM; US ADULTS; WOMEN; PROJECT; PREVENTION AB We piloted a 6-month, church-based, behavioral intervention, Delta Body and Soul (DBS), for African American (AA) adults in the Lower Mississippi Delta (LMD). DBS was designed to improve overall dietary quality in LMD AA adults. The intervention included six once monthly group-based educational sessions implemented by trained church members. Program implementation, session attendance, congregational feedback, and baseline and post-intervention, demographic, health, behavioral, and clinical parameters were assessed. Participants were predominately AA, female, and overweight or obese. Retention rate was 79 %. High adherence, defined as attendance at four or more educational sessions, was associated with dietary quality improvement and reduced blood glucose. Implementation of the DBS pilot intervention was feasible and may result in dietary quality and clinical improvements. C1 [Tussing-Humphreys, Lisa M.] Univ Illinois, Dept Med, Div Hlth Promot Res, Chicago, IL 60608 USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Ctr Canc, Chicago, IL 60608 USA. [Thomson, Jessica L.] ARS, Mid South Area, USDA, Stoneville, MS 38776 USA. [Onufrak, Stephen J.] Ctr Dis Control & Prevent, Obes Prevent & Control Branch, Atlanta, GA 30341 USA. RP Tussing-Humphreys, LM (reprint author), Univ Illinois, Dept Med, Div Hlth Promot Res, Chicago, IL 60608 USA. EM ltussing@uic.edu NR 37 TC 6 Z9 6 U1 1 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0022-4197 EI 1573-6571 J9 J RELIG HEALTH JI J. Relig. Health PD APR PY 2015 VL 54 IS 2 BP 455 EP 469 DI 10.1007/s10943-014-9823-5 PG 15 WC Public, Environmental & Occupational Health; Religion SC Public, Environmental & Occupational Health; Religion GA CB4NO UT WOS:000349604800008 PM 24442772 ER PT J AU Lee, J Hwang, YS Kang, IK Choung, MG AF Lee, Jinwook Hwang, Young-Sun Kang, In-Kyu Choung, Myoung-Gun TI Lipophilic pigments differentially respond to drying methods in tea (Camellia sinensis L.) leaves SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Chlorophyll; Lutein; beta-Carotene; Pheophytin; Principal component analysis (PCA) ID NON-POLYPHENOLIC FRACTION; GREEN TEA; CHLOROPHYLLASE; CAROTENOIDS; COMPONENTS; CHEMISTRY; QUALITY; AROMA AB This study evaluated the effectiveness of drying methods in tea leaves on lipid soluble pigments. Tea leaves were conventionally steamed, freeze-dried, room temperature-dried, oven-dried, or microwave-dried and afterward 6 lipophilic pigments were identified using the high performance liquid chromatography approach. While conventional drying resulted in evenly distributed total higher pigment metabolites, the freeze drying method resulted in highest levels of chlorophyll, lutein, and beta-carotene. Room temperature drying resulted in the lowest contents of pigment metabolites, and the microwave drying method had uniformly moderate levels of metabolites. The levels of these lipophilic pigments were visualized using a heatmap and co-correlated according to the drying method. The principal component analysis scores plot shows that a relatively close linkage appeared between fresh leaves and the room temperature drying method as well as between the conventional and microwave drying methods. The conventional, freeze, room temperature, and microwave drying methods resulted in differential correlations among the pigment metabolites; in other words, drying method affects final ratios of pigment metabolites amongst themselves. Both the freeze drying method as well as the microwave drying method appear to better maintain pigment metabolite contents, compared to the conventional drying method, and could replace the conventional method for these reasons. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Lee, Jinwook] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Lee, Jinwook; Kang, In-Kyu] Kyungpook Natl Univ, Dept Hort Sci, Taegu 702701, South Korea. [Hwang, Young-Sun; Choung, Myoung-Gun] Kangwon Natl Univ, Dept Herbal Med Resource, Dogye Up 245907, Samcheok, South Korea. [Hwang, Young-Sun] Univ Texas Arlington, Dept Biol, Arlington, TX 76019 USA. RP Choung, MG (reprint author), Kangwon Natl Univ, Dept Herbal Med Resource, Dogye Campus,Hwangjori 3, Dogye Up 245907, Samcheok, South Korea. EM cmg7004@kangwon.ac.kr NR 27 TC 3 Z9 4 U1 7 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD APR PY 2015 VL 61 IS 1 BP 201 EP 208 DI 10.1016/j.lwt.2014.11.025 PG 8 WC Food Science & Technology SC Food Science & Technology GA CA5MS UT WOS:000348953100029 ER PT J AU Canto, ACVCS Suman, SP Nair, MN Li, ST Rentfrow, G Beach, CM Silva, TJP Wheeler, TL Shackelford, SD Grayson, A McKeith, RO King, DA AF Canto, Anna C. V. C. S. Suman, Surendranath P. Nair, Mahesh N. Li, Shuting Rentfrow, Gregg Beach, Carol M. Silva, Teofilo J. P. Wheeler, Tommy L. Shackelford, Steven D. Grayson, Adria McKeith, Russell O. King, D. Andy TI Differential abundance of sarcoplasmic proteome explains animal effect on beef Longissimus lumborum color stability SO MEAT SCIENCE LA English DT Article DE Beef color; Color stability; Glycolytic enzyme; Longissimus lumborum; Myoglobin; Sarcoplasmic proteome ID INFLUENCING METMYOGLOBIN FORMATION; OXIDATION-INDUCED OXIDATION; MAMMALIAN SKELETAL-MUSCLE; MEAT QUALITY TRAITS; LIPID OXIDATION; BOVINE MUSCLES; PYRUVATE-KINASE; POSTTRANSLATIONAL MODIFICATIONS; DISCOLORATION CHARACTERISTICS; MASS-SPECTROMETRY AB The sarcoplasmic proteome of beef Longissimus lumborum demonstrating animal-to-animal variation in color stability was examined to correlate proteome profile with color. Longissimus lumborum (36 h post-mortem) muscles were obtained from 73 beef carcasses, aged for 13 days, and fabricated to 2.5-cm steaks. One steak was allotted to retail display, and another was immediately vacuum packaged and frozen at -80 degrees C. Aerobically packaged steaks were stored under display, and color was evaluated on days 0 and 11. The steaks were ranked based on redness and color stability on day 11, and ten color-stable and ten color-labile carcasses were identified. Sarcoplasmic proteome of frozen steaks from the selected carcasses was analyzed. Nine proteins were differentially abundant in color-stable and color-labile steaks. Three glycolytic enzymes (phosphoglucomutase-1, glyceraldehyde-3-phosphate dehydrogenase, and pyruvate kinase M2) were over-abundant in color-stable steaks and positively correlated (P<0.05) to redness and color stability. These results indicated that animal variations in proteome contribute to differences in beef color. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Canto, Anna C. V. C. S.; Suman, Surendranath P.; Nair, Mahesh N.; Li, Shuting; Rentfrow, Gregg] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Canto, Anna C. V. C. S.; Silva, Teofilo J. P.] Univ Fed Fluminense, Fac Vet, Dept Tecnol Alimentos, BR-24230340 Rio De Janeiro, Brazil. [Beach, Carol M.] Univ Kentucky, Prote Core Facil, Lexington, KY 40506 USA. [Wheeler, Tommy L.; Shackelford, Steven D.; Grayson, Adria; McKeith, Russell O.; King, D. Andy] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Suman, SP (reprint author), Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. EM spsuma2@uky.edu OI Suman, Surendranath/0000-0001-7523-2146 FU Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture [2012-67018-30166]; Office of the Vice President for Research; Coordination for the Improvement of Higher Level Personnel (CAPES, Brazil) [BEX 0128-12-0] FX This project was supported by the Agriculture and Food Research Initiative Grant 2012-67018-30166 from the USDA National Institute of Food and Agriculture. Mass spectrometric analysis was performed at the University of Kentucky's Proteomics Core Facility, supported in part by funds from the Office of the Vice President for Research. The authors thank the Coordination for the Improvement of Higher Level Personnel (CAPES, Brazil) for providing the PDSE scholarship (BEX 0128-12-0) to Anna Canto for completing doctoral research at the University of Kentucky. NR 81 TC 14 Z9 14 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1740 EI 1873-4138 J9 MEAT SCI JI Meat Sci. PD APR PY 2015 VL 102 BP 90 EP 98 DI 10.1016/j.meatsci.2014.11.011 PG 9 WC Food Science & Technology SC Food Science & Technology GA CB2AG UT WOS:000349428500012 PM 25556319 ER PT J AU Li, M Malladi, S Hurd, HS Goldsmith, TJ Brichta-Harhay, DM Loneragan, GH AF Li, Min Malladi, Sasidhar Hurd, H. Scott Goldsmith, Timothy J. Brichta-Harhay, Dayna M. Loneragan, Guy H. TI Salmonella spp. in lymph nodes of fed and cull cattle: Relative assessment of risk to ground beef SO FOOD CONTROL LA English DT Article DE Salmonella; Risk assessment; Ground beef; Lymph node; Carcass surface; Cattle ID ESCHERICHIA-COLI O157-H7; DIRECT PLATING METHODS; PROCESSING PLANTS; UNITED-STATES; LAIRAGE ENVIRONMENT; ASSESSMENT MODEL; PREVALENCE; CARCASSES; INTERVENTIONS; CONTAMINATION AB Ground beef products have been implicated as the vehicle for the transmission of Salmonella in a number of outbreaks. Although carcass surface interventions have proven effective, Salmonella contamination in ground beef still occurs. Recent studies indicate that deep tissue lymph nodes (DTLNs) may be an important source of Salmonella contamination in ground beef products. We developed a stochastic simulation model covering the pre-to post-harvest stages to assess the relative contribution of DUN as compared with carcass surface, to Salmonella in ground beef, and the impact of various pre-and postharvest interventions. The model addressed fed and cull cattle, and in high and low prevalence seasons. Contamination from carcass surfaces and DTLNs was simulated separately. Linear relationships were used to describe the changes of Salmonella surface concentration and prevalence at different processing stages. The baseline results indicate that on average over 90% of the Salmonella CFU load in a 2000 lb (907 kg) production lot originates from DUN contamination as compared with carcass surface contamination. The relative contribution of DUN contamination was fairly robust to changes in model parameters for ground beef from fed cattle, while it was comparatively more sensitive to changes in model parameters for cull cattle. The predicted mean Salmonella CFU load from DTLN contamination was considerably greater in ground beef production lots from fed cattle compared with cull cattle. Correspondingly, our scenario analysis suggested that generic pre-harvest interventions which can reduce Salmonella contamination in DTLNs would cause a greater total CFU load reduction in ground beef production lots from fed cattle compared with cull cattle. The study provides some valuable information for prioritizing control measures targeted at Salmonella contamination from the beef carcass surface or DTLNs based on the current knowledge. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Li, Min; Hurd, H. Scott] Iowa State Univ, Dept Prod Anim Med, Coll Vet Med, Ames, IA 50011 USA. [Malladi, Sasidhar; Goldsmith, Timothy J.] Univ Minnesota, Coll Vet Med, Ctr Anim Hlth & Food Safety, St Paul, MN 55108 USA. [Brichta-Harhay, Dayna M.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Loneragan, Guy H.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. RP Li, M (reprint author), Cornell Univ, Dept Populat Med & Diagnost Sci, Coll Vet Med, S3-110 Schurman Hall, Ithaca, NY 14853 USA. EM minli@cornell.edu FU Beef Checkoff; USDA National Institutes of Food and Agriculture [2011-51110-31081] FX This project is funded in part by the The Beef Checkoff and the USDA National Institutes of Food and Agriculture (Award No. 2011-51110-31081). The authors are grateful to Drs. Terrance M. Arthur and Joseph M. Bosilevac from USDA/ARS for kindly providing scientific advice and experimental data to define key model parameters, and Drs. Darby Murphy and Sarah E. Strayer from University of Minnesota for contributing to literature search and screening. The author Dr. H. Scott Hurd passed away before the manuscript submission. The rest of the authors acknowledge his significant contributions in designing, conducting, and preparing manuscript of this project. NR 40 TC 4 Z9 4 U1 2 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD APR PY 2015 VL 50 BP 423 EP 434 DI 10.1016/j.foodcont.2014.09.011 PG 12 WC Food Science & Technology SC Food Science & Technology GA AY4YO UT WOS:000347581100059 ER PT J AU Friedman, M Henika, PR Levin, CE AF Friedman, Mendel Henika, Philip R. Levin, Carol E. TI Antimicrobial activities of red wine-based formulations containing plant extracts against Escherichia coli O157:H7 and Salmonella enterica serovar Hadar SO FOOD CONTROL LA English DT Article DE Microbial food safety; Apple Poly (TM); Hidrox (TM); Lemongrass; Oregano; Bitter almond ID ORGANIC LEAFY GREENS; INACTIVATE ANTIBIOTIC-RESISTANT; LISTERIA-MONOCYTOGENES; ESSENTIAL OILS; OREGANO OIL; GROUND-BEEF; CAMPYLOBACTER-JEJUNI; FOODBORNE PATHOGENS; CLOSTRIDIUM-PERFRINGENS; BACTERICIDAL ACTIVITIES AB We evaluated the antimicrobial activities of three red wine based plant extract/plant essential oil (EO) formulations: olive extract powder/oregano EO, apple skin extract powder/lemongrass EO), and green tea extract powder/bitter almond EO, and their formula permutations against the foodborne pathogens Escherichia coli O157:H7 and Salmonella enterica serovar Hadar. The following parameters that were expected to affect antimicrobial activity were evaluated: pH (3.6 or 7.0), temperature (4, 22, and 37 degrees C), incubation time (0, 5, 30, and 60 min) and bacterial load (similar to 10(4) or similar to 10(9) bacteria/ml). The formulations with oregano, lemongrass, or bitter almond EO inhibited the growth of both pathogens at acidic pH with bacterial loads of similar to 10(4) or similar to 10(9) bacteria/ml. At neutral pH, the formulations were less effective. Although all the 3-component formulations were fast acting; showing significant activity in less than 5 min, only the activity of the lemongrass-containing formulation markedly increased with longer incubation times from 0 to 60 min. Activity also increased for all 3-component formulations with increasing temperature from 4 degrees C to 37 degrees C, although the activity of the lemongrass formulation appeared to level off at 22 degrees C. Of the tested formulations, the lemongrass EO formulations, at pH 3.6, appeared to be the most effective against the tested pathogens, especially against Salmonella. The most active formulations merit evaluation for antimicrobial efficacy in liquid and solid food. Published by Elsevier Ltd. C1 [Friedman, Mendel; Henika, Philip R.; Levin, Carol E.] USDA ARS, Prod Safety & Microbiol Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. RP Friedman, M (reprint author), USDA ARS, Prod Safety & Microbiol Res Unit, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM mendel.friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 NR 33 TC 3 Z9 4 U1 4 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD APR PY 2015 VL 50 BP 652 EP 658 DI 10.1016/j.foodcont.2014.10.005 PG 7 WC Food Science & Technology SC Food Science & Technology GA AY4YO UT WOS:000347581100090 ER PT J AU Lacombe, A Niemira, BA Gurtler, JB Fan, XT Sites, J Boyd, G Chen, HQ AF Lacombe, Alison Niemira, Brendan A. Gurtler, Joshua B. Fan, Xuetong Sites, Joseph Boyd, Glenn Chen, Haiqiang TI Atmospheric cold plasma inactivation of aerobic microorganisms on blueberries and effects on quality attributes SO FOOD MICROBIOLOGY LA English DT Article DE Cold plasma; Blueberry; Microflora; Storage; Quality; Sensory attributes ID SALMONELLA-ENTERICA; DECONTAMINATION; O157-H7; FOODS; LIGHT AB Cold plasma (CP) is a novel nonthermal technology, potentially useful in food processing settings. Berries were treated with atmospheric CP for 0, 15, 30, 45, 60, 90, or 120 s at a working distance of 7.5 cm with a mixture of 4 cubic feet/minute (cfm) of CP jet and 7 cfm of ambient air. Blueberries were sampled for total aerobic plate count (APC) and yeast/molds immediately after treatment and at 1, 2, and 7 days. Blueberries were also analyzed for compression firmness, surface color, and total anthocyanins immediately after each treatment. All treatments with CP significantly (P < 0.05) reduced APC after exposure, with reductions ranging from 0.8 to 1.6 log CFU/g and 1.5 to 2.0 log CFU/g compared to the control after 1 and 7 days, respectively. Treatments longer than 60 s resulted in significant reductions in firmness, although it was demonstrated that collisions between the berries and the container contributed significantly to softening. A significant reduction in anthocyanins was observed after 90 s. The surface color measurements were significantly impacted after 120 s for the L* and a* values and 45 s for the b* values. CP can inactivate microorganisms on blueberries and could be optimized to improve the safety and quality of produce. Published by Elsevier Ltd. C1 [Lacombe, Alison; Niemira, Brendan A.; Gurtler, Joshua B.; Sites, Joseph; Boyd, Glenn] USDA, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Fan, Xuetong] USDA, Residue Chem & Predicat Microbiol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Chen, Haiqiang] Univ Delaware, Dept Food & Anim Sci, Newark, DE 19716 USA. RP Niemira, BA (reprint author), USDA, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, 600 Mermaid Lane, Wyndmoor, PA 19038 USA. EM Brendan.Niemira@ars.usda.gov OI Chen, Hsiu-Hsi/0000-0002-5799-6705 FU Agriculture and Food Research Competitive Grants Program of the USDA National Institute of Food and Agriculture, NIFA Award [2011-68003-30005] FX This project was supported by the Agriculture and Food Research Competitive Grants Program of the USDA National Institute of Food and Agriculture, NIFA Award No: 2011-68003-30005. The authors would also like to acknowledge Kimberly Sokorai for providing technical assistance in sensory analysis. NR 22 TC 17 Z9 17 U1 6 U2 82 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD APR PY 2015 VL 46 BP 479 EP 484 DI 10.1016/j.fm.2014.09.010 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA AW0OM UT WOS:000345992200059 PM 25475318 ER PT J AU Sim, SB Calla, B Hall, B DeRego, T Geib, SM AF Sim, Sheina B. Calla, Bernarda Hall, Brian DeRego, Theodore Geib, Scott M. TI Reconstructing a comprehensive transcriptome assembly of a white-pupal translocated strain of the pest fruit fly Bactrocera cucurbitae SO GIGASCIENCE LA English DT Article DE Bactrocera cucurbitae; Translocation; RNA-Seq; White-pupae; Tephritidae; Melon fly; SIT; Sterile insect technique ID RNA-SEQ DATA; REFERENCE GENOME AB Background: Bactrocera cucurbitae is a serious global agricultural pest. Basic genomic information is lacking for this species, and this would be useful to inform methods of control, damage mitigation, and eradication efforts. Here, we have sequenced, assembled, and annotated a comprehensive transcriptome for a mass-rearing sexing strain of this species. This forms a foundational genomic and transcriptomic resource that can be used to better understand the physiology and biochemistry of this insect as well as being a useful tool for population genetics. Findings: A transcriptome assembly was constructed containing 17,654 transcript isoforms derived from 10,425 unigenes. This transcriptome size is similar to reports from other Tephritid species and probably includes about 70-80% of the protein-coding genes in the genome. The dataset is publicly available in NCBI and GigaDB as a resource for researchers. Conclusions: Foundational knowledge on the protein-coding genes in B. cucurbitae will lead to improved resources for this species. Through comparison with a model system such as Drosophila as well as a growing number of related Tephritid transcriptomes, improved strategies can be developed to control this pest. C1 [Sim, Sheina B.; Calla, Bernarda; Hall, Brian; DeRego, Theodore; Geib, Scott M.] ARS, Trop Crop & Commod Protect Res Unit, Daniel K Inouye US Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. [Sim, Sheina B.; Hall, Brian] Univ Hawaii Manoa, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA. RP Geib, SM (reprint author), ARS, Trop Crop & Commod Protect Res Unit, Daniel K Inouye US Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. EM scott.geib@ars.usda.gov OI Geib, Scott/0000-0002-9511-5139 FU USDA-ARS; USDA Farm Bill Project [3.0251]; National Science Foundation [OCI-1053575XSEDE, TG-MCB140032] FX We thank Steven Tam for assistance in colony rearing and fruit fly sample collections used in this study. Funding was provided by USDA-ARS and SBS and BH were supported by USDA Farm Bill Project 3.0251. Bioinformatic analysis was performed on computing resources at USDA-ARS Pacific Basin Agricultural Research Center (Moana cluster; Hilo, HI, http://moana.dnsalias.org) and the Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by National Science Foundation grant number OCI-1053575XSEDE utilizing allocation TG-MCB140032 to SMG. Opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. USDA is an equal opportunity provider and employer. NR 11 TC 2 Z9 3 U1 1 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND EI 2047-217X J9 GIGASCIENCE JI GigaScience PD MAR 31 PY 2015 VL 4 AR 14 DI 10.1186/s13742-015-0053-x PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX4IK UT WOS:000365663100001 PM 25830018 ER PT J AU Steele, LD Coates, B Valero, MC Sun, W Seong, KM Muir, WM Clark, JM Pittendrigh, BR AF Steele, Laura D. Coates, Brad Valero, M. Carmen Sun, Weilin Seong, Keon Mook Muir, William M. Clark, John M. Pittendrigh, Barry R. TI Selective Sweep Analysis in the Genomes of the 91-R and 91-C Drosophila melanogaster Strains Reveals Few of the 'Usual Suspects' in Dichlorodiphenyltrichloroethane (DDT) Resistance SO PLOS ONE LA English DT Article ID LONG TERMINAL REPEAT; SODIUM-CHANNEL GENE; INSECTICIDE RESISTANCE; CROSS-RESISTANCE; AEDES-AEGYPTI; CYTOCHROME-P-450 SYSTEM; SUSCEPTIBLE STRAINS; ANOPHELES-GAMBIAE; POINT MUTATIONS; WIDE ANALYSIS AB Adaptation of insect phenotypes for survival after exposure to xenobiotics can result from selection at multiple loci with additive genetic effects. To the authors' knowledge, no selective sweep analysis has been performed to identify such loci in highly dichlorodiphenyltrichloroethane (DDT) resistant insects. Here we compared a highly DDT resistant phenotype in the Drosophila melanogaster (Drosophila) 91-R strain to the DDT susceptible 91-C strain, both of common origin. Whole genome re-sequencing data from pools of individuals was generated separately for 91-R and 91-C, and mapped to the reference Drosophila genome assembly (v. 5.72). Thirteen major and three minor effect chromosome intervals with reduced nucleotide diversity (pi) were identified only in the 91-R population. Estimates of Tajima's D (D) showed corresponding evidence of directional selection in these same genome regions of 91-R, however, no similar reductions in p or D estimates were detected in 91-C. An overabundance of non-synonymous proteins coding to synonymous changes were identified in putative open reading frames associated with 91-R. Except for NinaC and Cyp4g1, none of the identified genes were the 'usual suspects' previously observed to be associated with DDT resistance. Additionally, up-regulated ATP-binding cassette transporters have been previously associated with DDT resistance; however, here we identified a structurally altered MDR49 candidate resistance gene. The remaining fourteen genes have not previously been shown to be associated with DDT resistance. These results suggest hitherto unknown mechanisms of DDT resistance, most of which have been overlooked in previous transcriptional studies, with some genes having orthologs in mammals. C1 [Steele, Laura D.; Valero, M. Carmen; Sun, Weilin; Seong, Keon Mook; Pittendrigh, Barry R.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Coates, Brad] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Muir, William M.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Clark, John M.] Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA. RP Steele, LD (reprint author), Univ Illinois, Dept Entomol, 320 Morrill Hall, Urbana, IL 61801 USA. EM steele11@illinois.edu FU United States Department of Agriculture, Agricultural Research Service (USDA-ARS; CRIS Project) [3625-22000-017-00]; Iowa Agriculture and Home Economics Experiment Station, Ames, IA [3543]; C.W. Kearns, C.L. Metcalf and W.P. Flint Endowment FX The sequencing of the 91-R and 91-C genomes were paid for by the C.W. Kearns, C.L. Metcalf and W.P. Flint Endowment. A portion of research data analyses was supported by the United States Department of Agriculture, Agricultural Research Service (USDA-ARS; CRIS Project 3625-22000-017-00) and the Iowa Agriculture and Home Economics Experiment Station, Ames, IA (Project 3543). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 91 TC 3 Z9 3 U1 4 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 31 PY 2015 VL 10 IS 3 AR e0123066 DI 10.1371/journal.pone.0123066 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE8IF UT WOS:000352084800105 PM 25826265 ER PT J AU Tuggle, CK Waters, WR AF Tuggle, Christopher K. Waters, W. Ray TI Tuberculosis-resistant transgenic cattle SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Editorial Material ID BOVINE TUBERCULOSIS; GENETIC SUSCEPTIBILITY; MYCOBACTERIUM-BOVIS; INNATE IMMUNITY C1 [Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Waters, W. Ray] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Tuggle, CK (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. EM cktuggle@iastate.edu NR 19 TC 3 Z9 3 U1 0 U2 12 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 31 PY 2015 VL 112 IS 13 BP 3854 EP 3855 DI 10.1073/pnas.1502972112 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE5ZH UT WOS:000351914500031 PM 25829535 ER PT J AU Sun, YX AF Sun, Yuxiang TI Ghrelin receptor controls obesity by fat burning SO ONCOTARGET LA English DT Editorial Material ID BROWN ADIPOSE-TISSUE; WHITE; AGE C1 ARS, USDA, Childrens Nutr Res Ctr, Huffington Ctr Aging,Baylor Coll Med, Houston, TX 77041 USA. RP Sun, YX (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Huffington Ctr Aging,Baylor Coll Med, Houston, TX 77041 USA. EM yuxiangs@bcm.edu NR 8 TC 1 Z9 1 U1 0 U2 0 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD MAR 30 PY 2015 VL 6 IS 9 BP 6470 EP 6471 PG 2 WC Oncology; Cell Biology SC Oncology; Cell Biology GA CF8GI UT WOS:000352793800001 PM 25823665 ER PT J AU Jung, SY Vitolins, MZ Fenton, J Frazier-Wood, AC Hursting, SD Chang, SN AF Jung, Su Yon Vitolins, Mara Z. Fenton, Jenifer Frazier-Wood, Alexis C. Hursting, Stephen D. Chang, Shine TI Risk Profiles for Weight Gain among Postmenopausal Women: A Classification and Regression Tree Analysis Approach SO PLOS ONE LA English DT Article ID CORONARY-HEART-DISEASE; BODY-MASS INDEX; BREAST-CANCER; PHYSICAL-ACTIVITY; LIFE-STYLE; OBESITY; HEALTH; SMOKING; FAT; DIET AB Purpose Risk factors for obesity and weight gain are typically evaluated individually while "adjusting for" the influence of other confounding factors, and few studies, if any, have created risk profiles by clustering risk factors. We identified subgroups of postmenopausal women homogeneous in their clustered modifiable and non-modifiable risk factors for gaining >= 3% weight. Methods This study included 612 postmenopausal women 50-79 years old, enrolled in an ancillary study of the Women's Health Initiative Observational Study between February 1995 and July 1998. Classification and regression tree and stepwise regression models were built and compared. Results Of 27 selected variables, the factors significantly related to >= 3% weight gain were weight change in the past 2 years, age at menopause, dietary fiber, fat, alcohol intake, and smoking. In women younger than 65 years, less than 4 kg weight change in the past 2 years sufficiently reduced risk of >= 3% weight gain. Different combinations of risk factors related to weight gain were reported for subgroups of women: women 65 years or older (essential factor: < 9.8 g/day dietary factor), African Americans (essential factor: currently smoking), and white women (essential factor: >= 5 kg weight change for the past 2 years). Conclusions Our findings suggest specific characteristics for particular subgroups of postmenopausal women that may be useful for identifying those at risk for weight gain. The study results may be useful for targeting efforts to promote strategies to reduce the risk of obesity and weight gain in subgroups of postmenopausal women and maximize the effect of weight control by decreasing obesity-relevant adverse health outcomes. C1 [Jung, Su Yon; Chang, Shine] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Sch Nursing, Translat Sci Sect, Los Angeles, CA 90024 USA. [Vitolins, Mara Z.] Wake Forest Sch Med, Div Publ Hlth Sci, Dept Epidemiol & Prevent, Winston Salem, NC USA. [Fenton, Jenifer] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Frazier-Wood, Alexis C.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Hursting, Stephen D.] Univ Texas Austin, Dept Nutr Sci, Austin, TX 78712 USA. RP Jung, SY (reprint author), Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Sch Nursing, Translat Sci Sect, Los Angeles, CA 90024 USA. EM sjung@sonnet.ucla.edu FU NCI [R21 CA086036] FX This study was supported by an NCI grant (R21 CA086036; J.H. Grudo, Principal Investigator). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 4 Z9 4 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 30 PY 2015 VL 10 IS 3 AR e0121430 DI 10.1371/journal.pone.0121430 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE9AD UT WOS:000352134700104 PM 25822239 ER PT J AU Luo, J Qiu, H Cai, GH Wagner, NE Bhattacharya, D Zhang, N AF Luo, Jing Qiu, Huan Cai, Guohong Wagner, Nicole E. Bhattacharya, Debashish Zhang, Ning TI Phylogenomic analysis uncovers the evolutionary history of nutrition and infection mode in rice blast fungus and other Magnaporthales SO SCIENTIFIC REPORTS LA English DT Article ID MOLECULAR PHYLOGENY; COALESCENT MODEL; FRESH-WATER; SEQUENCE; CLASSIFICATION; ASCOMYCOTA; GENOME; PEZIZOMYCOTINA; BOOTSTRAP; INFERENCE AB The order Magnaporthales (Ascomycota, Fungi) includes devastating pathogens of cereals, such as the rice blast fungus Pyricularia (Magnaporthe) oryzae, which is a model in host-pathogen interaction studies. Magnaporthales also includes saprotrophic species associated with grass roots and submerged wood. Despite its scientific and economic importance, the phylogenetic position of Magnaporthales within Sordariomycetes and the interrelationships of its constituent taxa, remain controversial. In this study, we generated novel transcriptome data from 21 taxa that represent key Magnaporthales lineages of different infection and nutrition modes and phenotypes. Phylogenomic analysis of > 200 conserved genes allowed the reconstruction of a robust Sordariomycetes tree of life that placed the monophyletic group of Magnaporthales sister to Ophiostomatales. Among Magnaporthales, three major clades were recognized: 1) an early diverging clade A comprised of saprotrophs associated with submerged woods; 2) clade B that includes the rice blast fungus and other pathogens that cause blast diseases of monocot plants. These species infect the above-ground tissues of host plants using the penetration structure, appressorium; and 3) clade C comprised primarily of root-associated species that penetrate the root tissue with hyphopodia. The well-supported phylogenies provide a robust framework for elucidating evolution of pathogenesis, nutrition modes, and phenotypic characters in Magnaporthales. C1 [Luo, Jing; Zhang, Ning] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Qiu, Huan; Wagner, Nicole E.; Bhattacharya, Debashish] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. [Cai, Guohong] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Zhang, Ning] Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08901 USA. RP Bhattacharya, D (reprint author), Rutgers State Univ, Dept Ecol Evolut & Nat Resources, Foran Hall 102,59 Dudley Rd, New Brunswick, NJ 08901 USA. EM debash.bhattacharya@gmail.com; zhang@aesop.rutgers.edu RI Qiu, Huan/C-2671-2016; Zhang, Ning/K-3046-2012 OI Zhang, Ning/0000-0003-0755-2505 FU National Science Foundation [DEB 1145174] FX This work was supported by a grant from the National Science Foundation (DEB 1145174) awarded to N. Zhang and D. Bhattacharya. We thank Udi Zelzion (Rutgers University) for assistance in assembling the transcriptome data. NR 50 TC 11 Z9 11 U1 3 U2 27 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 30 PY 2015 VL 5 AR 9448 DI 10.1038/srep09448 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE6HS UT WOS:000351938100002 PM 25819715 ER PT J AU Yanai, RD Tokuchi, N Campbell, JL Green, MB Matsuzaki, E Laseter, SN Brown, CL Bailey, AS Lyons, P Levine, CR Buso, DC Likens, GE Knoepp, JD Fukushima, K AF Yanai, Ruth D. Tokuchi, Naoko Campbell, John L. Green, Mark B. Matsuzaki, Eiji Laseter, Stephanie N. Brown, Cindi L. Bailey, Amey S. Lyons, Pilar Levine, Carrie R. Buso, Donald C. Likens, Gene E. Knoepp, Jennifer D. Fukushima, Keitaro TI Sources of uncertainty in estimating stream solute export from headwater catchments at three sites SO HYDROLOGICAL PROCESSES LA English DT Article DE Gomadansan; Hubbard Brook; Coweeta Experimental Forest; nutrient flux; model selection; gap filling; water chemistry; measurement error ID BROOK-EXPERIMENTAL-FOREST; WATER-QUALITY DATA; LOAD ESTIMATION; MODEL CALIBRATION AB Uncertainty in the estimation of hydrologic export of solutes has never been fully evaluated at the scale of a small-watershed ecosystem. We used data from the Gomadansan Experimental Forest, Japan, Hubbard Brook Experimental Forest, USA, and Coweeta Hydrologic Laboratory, USA, to evaluate many sources of uncertainty, including the precision and accuracy of measurements, selection of models, and spatial and temporal variation. Uncertainty in the analysis of stream chemistry samples was generally small but could be large in relative terms for solutes near detection limits, as is common for ammonium and phosphate in forested catchments. Instantaneous flow deviated from the theoretical curve relating height to discharge by up to 10% at Hubbard Brook, but the resulting corrections to the theoretical curve generally amounted to <0.5% of annual flows. Calibrations were limited to low flows; uncertainties at high flows were not evaluated because of the difficulties in performing calibrations during events. However, high flows likely contribute more uncertainty to annual flows because of the greater volume of water that is exported during these events. Uncertainty in catchment area was as much as 5%, based on a comparison of digital elevation maps with ground surveys. Three different interpolation methods are used at the three sites to combine periodic chemistry samples with streamflow to calculate fluxes. The three methods differed by <5% in annual export calculations for calcium, but up to 12% for nitrate exports, when applied to a stream at Hubbard Brook for 1997-2008; nitrate has higher weekly variation at this site. Natural variation was larger than most other sources of uncertainty. Specifically, coefficients of variation across streams or across years, within site, for runoff and weighted annual concentrations of calcium, magnesium, potassium, sodium, sulphate, chloride, and silicate ranged from 5 to 50% and were even higher for nitrate. Uncertainty analysis can be used to guide efforts to improve confidence in estimated stream fluxes and also to optimize design of monitoring programmes. (c) 2014 The Authors. Hydrological Processes published John Wiley & Sons, Ltd. C1 [Yanai, Ruth D.; Lyons, Pilar; Levine, Carrie R.] SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. [Tokuchi, Naoko] Kyoto Univ, Div Forest Biosphere, Kyoto, Japan. [Campbell, John L.] US Forest Serv, No Res Stn, USDA, Durham, NH USA. [Green, Mark B.] Plymouth State Univ, Ctr Environm, Plymouth, NH USA. [Matsuzaki, Eiji] Univ British Columbia, Nat Resources & Environm Studies Grad Program, Prince George, BC, Canada. [Laseter, Stephanie N.; Brown, Cindi L.; Knoepp, Jennifer D.] US Forest Serv, Coweeta Hydrol Lab, USDA, Southern Res Stn, Otto, NC USA. [Bailey, Amey S.] US Forest Serv, Hubbard Brook Expt Forest, USDA, No Res Stn, North Woodstock, NH USA. [Buso, Donald C.] Cary Inst Ecosyst Studies, Hubbard Brook Expt Forest, North Woodstock, NH USA. [Likens, Gene E.] Cary Inst, Millbrook, NY USA. [Fukushima, Keitaro] Kyoto Univ, Grad Sch Agr, Kyoto, Japan. RP Yanai, RD (reprint author), SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. EM rdyanai@syr.edu OI Campbell, John/0000-0003-4956-1696; Green, Mark/0000-0002-7415-7209 FU National Science Foundation (NSF); NSF Long-Term Ecological Research (LTER) Network Office; Japanese Society for the Promotion of Science; US Forest Service, Department of Agriculture, Southern Research Station; NSF LTER [DEB-9632854, DEB-0218001]; A.W. Mellon Foundation; NSF, LTER programme; NSF, Long-Term Research in Environmental Biology programme FX This paper is a product of Quantifying Uncertainty in Ecosystem Studies (QUEST), www.quantifyinguncertainty.org, which was supported by the National Science Foundation (NSF) and the NSF Long-Term Ecological Research (LTER) Network Office. Many of the ideas implemented in this paper were developed in a meeting of a QUEST Working Group in March 2011. In June 2011, the Japanese Society for the Promotion of Science awarded a fellowship to Ruth Yanai, hosted by Naoko Tokuchi, to work on this project. Masanori Katsuyama and Nobu Ohte applied the hydrological model at Gomadansan. Tom Luther interpolated the precipitation volumes. Zak Krug entered data from the Coweeta archives handwritten in decades past, and Bill O'Neill and Adam Wild helped produce the figures. Wayne Martin, Jamie Shanley, and Doug Burns provided helpful reviews of the manuscript.; The research at Gomadansan is supported by the staff of the Wakayama Experimental Forest of Kyoto University. The Hubbard Brook Experimental Forest is operated by the US Forest Service, and it forms part of the NSF LTER network. Research at Coweeta Hydrologic Laboratory was funded by the US Forest Service, Department of Agriculture, Southern Research Station, and the NSF LTER (DEB-9632854 and DEB-0218001). Chemistry data for Hubbard Brook were obtained through funding to G.E. Likens from the A.W. Mellon Foundation and the NSF, including the LTER and Long-Term Research in Environmental Biology programmes. NR 33 TC 7 Z9 7 U1 6 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD MAR 30 PY 2015 VL 29 IS 7 BP 1793 EP 1805 DI 10.1002/hyp.10265 PG 13 WC Water Resources SC Water Resources GA CE0AJ UT WOS:000351463600009 ER PT J AU Tilahun, SA Guzman, CD Zegeye, AD Dagnew, DC Collick, AS Yitaferu, B Steenhuis, TS AF Tilahun, Seifu A. Guzman, Christian D. Zegeye, Assefa D. Dagnew, Dessalegn C. Collick, Amy S. Yitaferu, Birru Steenhuis, Tammo S. TI Distributed discharge and sediment concentration predictions in the sub-humid Ethiopian highlands: the Debre Mawi watershed SO HYDROLOGICAL PROCESSES LA English DT Article DE erosion; saturation excess runoff; sediment transport; gully erosion; simulation models; Blue Nile; Ethiopia ID MARA RIVER-BASIN; SOIL-EROSION; LAKE TANA; BLUE NILE; NORTHERN ETHIOPIA; BALANCE MODEL; LAND-USE; RUNOFF; IMPACT; YIELD AB Experimental research in the Ethiopian highlands found that saturation excess induced runoff and erosion are common in the sub-humid conditions. Because most erosion simulation models applied in the highlands are based on infiltration excess, we, as an alternative, developed the Parameter Efficient Distributed (PED) model, which can simulate water and sediment fluxes in landscapes with saturation excess runoff. The PED model has previously only been tested at the outlet of a watershed and not for distributed runoff and sediment concentration within the watershed. In this study, we compare the distributed storm runoff and sediment concentration of the PED model against collected data in the 95-ha Debre Mawi watershed and three of its nested sub-watersheds for the 2010 and 2011 rainy seasons. In the PED model framework, the hydrology of the watershed is divided between infiltrating and runoff zones, with erosion only taking place from two surface runoff zones. Daily storm runoff and sediment concentration values, ranging from 0.5 to over 30mm and from 0.1 to 35gl(-1), respectively, were well simulated. The Nash Sutcliffe efficiency values for the daily storm runoff for outlet and sub-watersheds ranged from 0.66 to 0.82, and the Nash-Sutcliffe efficiency for daily sediment concentrations were greater than 0.78. Furthermore, the model uses realistic fractional areas for surface and subsurface flow contributions, for example between saturated areas (15%), degraded areas (30%) and permeable areas (55%) at the main outlet, while close similarity was found for the remaining hydrology and erosion parameter values. One exception occurred for the distinctly greater transport limited parameter at the actively gullying lower part of the watershed. The results suggest that the model based on saturation excess provides a good representation of the observed spatially distributed runoff and sediment concentrations within a watershed by modelling the bottom lands (as opposed to the uplands) as the dominant contributor of the runoff and sediment load. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Tilahun, Seifu A.; Dagnew, Dessalegn C.; Steenhuis, Tammo S.] Bahir Dar Univ, Sch Civil & Water Resources Engn, Bahir Dar, Ethiopia. [Guzman, Christian D.; Zegeye, Assefa D.; Steenhuis, Tammo S.] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY USA. [Collick, Amy S.] ARS, USDA, University Pk, PA USA. [Zegeye, Assefa D.] ARARI, Adet Res Ctr, Bahir Dar, Ethiopia. [Yitaferu, Birru] ARARI, Bahir Dar, Ethiopia. RP Tilahun, SA (reprint author), Bahir Dar Univ, Sch Civil & Water Resources Engn, Bahir Dar, Ethiopia. EM satadm86@gmail.com FU International Foundation for Science (IFS); Higher Education for Development (HED); USDA International Science and Education (ISE) program; PEER Science Program of USAID FX Funding of the assistantship of the senior author was made available by the International Foundation for Science (IFS), Higher Education for Development (HED), USDA International Science and Education (ISE) program and PEER Science Program of USAID. The runoff and sediment data at the outlet were made available by Amhara Regional Agricultural Research Institute. NR 46 TC 6 Z9 6 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD MAR 30 PY 2015 VL 29 IS 7 BP 1817 EP 1828 DI 10.1002/hyp.10298 PG 12 WC Water Resources SC Water Resources GA CE0AJ UT WOS:000351463600011 ER PT J AU Shin, WK Yokoyama, WH Kim, W Wicker, L Kim, Y AF Shin, Woo-kyoung Yokoyama, Wallace H. Kim, Wook Wicker, Louise Kim, Yookyung TI Change in texture improvement of low-fat tofu by means of low-fat soymilk protein denaturation SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE low-fat tofu; low-fat soymilk; stepwise denaturation; gelling ID SELECTIVE THERMAL-DENATURATION; BETA-LACTOGLOBULIN; SOYBEAN VARIETIES; SOY PROTEIN; AGGREGATION; ACID; CURD AB BACKGROUNDTofu made from low-fat soy flour is a nutritional food for consumers and economically benefits the food processor. However, low-fat tofu has poor textural quality, especially insufficient firmness. Stepwise heating (heating at 75 degrees C, followed by holding at 95 degrees C) of full-fat soymilk increases gel properties. Therefore we evaluated the two-step heating of low-fat soymilk to improve tofu texture. RESULTSThe denaturation enthalpy and temperature of -conglycinin and glycinin were higher in low-fat tofu compared to high-fat tofu. The viscosity of low-fat soymilk and texture of tofu by one-step heating were weaker than full-fat soymilk and tofu. However, the two-step heating increased free sulfhydryl groups and viscosity of low-fat soymilk to a value higher or similar to conventional soymilk. The syneresis of low-fat tofu was reduced about 30% and hardness was higher (131.0 N) by the two-step process compared to one-step heating of full-fat tofu (101.4 N) by the one-step process. The microstructure of low-fat tofu became finer, denser and more homogeneous by the two-step heat process. CONCLUSIONLow-fat tofu produced by denaturing the two major soy proteins separately had improved textural qualities similar to full-fat tofu as a result of increased hydrophobic interactions between denatured protein molecules. (c) 2014 Society of Chemical Industry C1 [Shin, Woo-kyoung; Wicker, Louise; Kim, Yookyung] Korea Univ, Dept Human Ecol, Grad Sch, Seoul 136701, South Korea. [Yokoyama, Wallace H.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Kim, Wook] Korea Univ, Div Biotechnol, Seoul 136701, South Korea. [Wicker, Louise] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. RP Kim, Y (reprint author), Korea Univ, Dept Human Ecol, Grad Sch, Seoul 136701, South Korea. EM yookyung_kim@korea.ac.kr FU Korea Institute of Planning and Evaluation for Technology of Food, Agriculture, Forestry and Fisheries [108142-3] FX This research was supported by Korea Institute of Planning and Evaluation for Technology of Food, Agriculture, Forestry and Fisheries (No. 108142-3). NR 37 TC 1 Z9 1 U1 5 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD MAR 30 PY 2015 VL 95 IS 5 BP 1000 EP 1007 DI 10.1002/jsfa.6780 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CD5ZQ UT WOS:000351168100016 PM 24924689 ER PT J AU Rife, TW Wu, SY Bowden, RL Poland, JA AF Rife, Trevor W. Wu, Shuangye Bowden, Robert L. Poland, Jesse A. TI Spiked GBS: a unified, open platform for single marker genotyping and whole-genome profiling SO BMC GENOMICS LA English DT Article DE Plant breeding; Wheat; Marker platform; Genotyping-by-sequencing ID ASSISTED SELECTION; WHEAT; RESISTANCE; DISCOVERY; PLANTS; LOCUS; PCR; MAP AB Background: In plant breeding, there are two primary applications for DNA markers in selection: 1) selection of known genes using a single marker assay (marker-assisted selection; MAS); and 2) whole-genome profiling and prediction (genomic selection; GS). Typically, marker platforms have addressed only one of these objectives. Results: We have developed spiked genotyping-by-sequencing (sGBS), which combines targeted amplicon sequencing with reduced representation genotyping-by-sequencing. To minimize the cost of targeted assays, we utilize a small percent of sequencing capacity available in runs of GBS libraries to "spike" amplified targets of a priori alleles tagged with a different set of unique barcodes. This open platform allows multiple, single-target loci to be assayed while simultaneously generating a whole-genome profile. This dual-genotyping approach allows different sets of samples to be evaluated for single markers or whole genome-profiling. Here, we report the application of sGBS on a winter wheat panel that was screened for converted KASP markers and newly-designed markers targeting known polymorphisms in the leaf rust resistance gene Lr34. Conclusions: The flexibility and low-cost of sGBS will enable a range of applications across genetics research. Specifically in breeding applications, the sGBS approach will allow breeders to obtain a whole-genome profile of important individuals while simultaneously targeting specific genes for a range of selection strategies across the breeding program. C1 [Rife, Trevor W.] Kansas State Univ, Interdept Genet, Manhattan, KS 66506 USA. [Rife, Trevor W.; Wu, Shuangye; Poland, Jesse A.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Manhattan, KS 66506 USA. [Bowden, Robert L.] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Poland, Jesse A.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Poland, JA (reprint author), Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, 4024 Throckmorton Hall, Manhattan, KS 66506 USA. EM jpoland@ksu.edu OI Rife, Trevor/0000-0002-5974-6523; Poland, Jesse/0000-0002-7856-1399 FU USDA-NIFA funded Triticeae Coordinated Agriculture Project [2011-68002-30029]; NSF [IIP-1338897]; USAID Feed the Future Innovation Lab for Applied Wheat Genomics [AID-OAA-A-13-00051]; Bill & Melinda Gates Foundation; United States Department of Agriculture-Agricultural Research Service [5430-21000-006-00D]; Kansas Wheat Alliance; Kansas Wheat Commission FX We would like to thank the USDA Central Small Grain Genotyping Lab in Manhattan, KS for sequencing. The USDA-NIFA funded Triticeae Coordinated Agriculture Project (T-CAP) (2011-68002-30029) provided support for TR. This work was completed under the auspices of WGRC I/UCRC partially funded by NSF grant contract (IIP-1338897) and the USAID Feed the Future Innovation Lab for Applied Wheat Genomics (Cooperative Agreement No. AID-OAA-A-13-00051). Partial funding for this research was provided by the Bill & Melinda Gates Foundation through a grant to Cornell University for "Genomic Selection: The next frontier for rapid gains in maize and wheat improvement", the United States Department of Agriculture-Agricultural Research Service (Appropriation #5430-21000-006-00D), the Kansas Wheat Alliance, and the Kansas Wheat Commission. Mention of trade names does not constitute endorsement by the U.S. Department of Agriculture. NR 34 TC 4 Z9 4 U1 5 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 28 PY 2015 VL 16 AR 248 DI 10.1186/s12864-015-1404-9 PG 7 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CG1DC UT WOS:000353012200001 PM 25880848 ER PT J AU Pieniazek, J Stipanovic, R Puckhaber, L Wedegaertner, T Farnell, M Byrd, J Lee, J AF Pieniazek, J. Stipanovic, R. Puckhaber, L. Wedegaertner, T. Farnell, M. Byrd, J. Lee, J. TI Pressed, dehulled cottonseed meal: Effect on growth performance in broiler diets and retention of gossypol in tissues SO EUROPEAN POULTRY SCIENCE LA English DT Article DE Broiler; nutrition; cottonseed meal; gossypol; performance ID (-)-GOSSYPOL; CHICKENS; SUPPLEMENTATION; (+)-GOSSYPOL; FORMULATION; ENANTIOMERS; LYSINE AB This experiment evaluated the effect of cottonseed meal on broiler growth performance and determined residual gossypol in chicken tissues. Pressed, dehulled cottonseed meal (CSM) was included at 5 and 10% either for the first 21 d or for the entire 41 d trial; body weights and FCR for birds fed the CSM were compared to those of birds fed an industry type maize-soybean meal diet. A starter diet was fed through 21 d, a grower diet for 22 to 35 d, and a finisher diet for 36 to 41 d. Body weight and FCR were determined on d 10, 21, 35, and 41. Muscle and liver samples were collected to determine the presence of gossypol on d 21, 35 and 41. At the conclusion of the trial, no differences were observed (P < 0.05) in body weight or FCR for birds fed any of the CSM diets compared to the control diet. Gossypol was not found in breast and thigh tissues from birds fed any of the CSM diets at 35 and 41 d. However, after 35 and 41 d gossypol was found in some of the liver samples. These data indicate that pressed, dehulled cottonseed meal can be included at up to 10% in broiler diets without negatively affecting growth performance. Further research should be conducted to ascertain the maximum level of CSM that may safely be included in broiler diets, and determine the presence of gossypol in chicken tissues. C1 [Pieniazek, J.; Lee, J.] Texas A&M Syst, Texas A&M AgriLife Res & Extens, Dept Poultry Sci, College Stn, TX USA. [Stipanovic, R.; Puckhaber, L.; Byrd, J.] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Wedegaertner, T.] Cotton Inc, Cary, NC USA. [Farnell, M.] Mississippi State Univ, Extens Serv, Dept Poultry Sci, Mississippi State, MS 39762 USA. RP Stipanovic, R (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. EM bob.stipanovic@ars.usda.gov NR 22 TC 1 Z9 1 U1 1 U2 12 PU EUGEN ULMER GMBH CO PI STUTTGART PA POSTFACH 700561 WOLLGRASWEG 41, D-70599 STUTTGART, GERMANY SN 1612-9199 J9 EUR POULTRY SCI JI Eur. Poult. Sci. PD MAR 27 PY 2015 VL 79 DI 10.1399/eps.2015.81 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CF9FH UT WOS:000352868400001 ER PT J AU Zhang, L Pan, P Sappington, TW Lu, WX Luo, LZ Jiang, XF AF Zhang, Lei Pan, Pan Sappington, Thomas W. Lu, Weixiang Luo, Lizhi Jiang, Xingfu TI Accelerated and Synchronized Oviposition Induced by Flight of Young Females May Intensify Larval Outbreaks of the Rice Leaf Roller SO PLOS ONE LA English DT Article ID CNAPHALOCROCIS-MEDINALIS LEPIDOPTERA; LONG-DURATION FLIGHT; TRADE-OFFS; MELANOPLUS-SANGUINIPES; CYDIA-POMONELLA; REPRODUCTION; MIGRATION; PYRALIDAE; FECUNDITY; BEHAVIOR AB Physiological management of migration-reproduction trade-offs in energy allocation often includes a package of adaptions referred to as the oogenesis-flight syndrome. In some species, this trade-off may be overestimated, because factors like flight behavior and environmental conditions may mitigate it. In this study, we examined the reproductive consequences induced by different flight scenarios in an economically-important Asian migrant insect, Cnaphalocrocis medinalis. We found that the influences of flight on reproduction are not absolutely positive or negative, but instead depend on the age at which the moth begins flight, flight duration, and how many consecutive nights they are flown. Adult flight on the 1st or 2nd night after emergence, flight for 6 h or 12 h nightly, and flight on the first two consecutive nights after emergence significantly accelerated onset of oviposition or enhanced synchrony of egg-laying. The latter can contribute to subsequent larval outbreaks. However, flight after the 3rd night, flight for 18 h at any age, or flight on more than 3 consecutive nights after adult emergence did not promote reproductive development, and in some scenarios even constrained adult reproduction. These results indicate that there is a migration/reproduction trade-off in C. medinalis, but that it is mitigated or eliminated by flight under appropriate conditions. The strategy of advanced and synchronized oviposition triggered by migratory flight of young females may be common in other migratory insect pests. C1 [Zhang, Lei; Pan, Pan; Lu, Weixiang; Luo, Lizhi; Jiang, Xingfu] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pest, Beijing 100193, Peoples R China. [Sappington, Thomas W.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Genet Lab, Ames, IA USA. RP Jiang, XF (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pest, Beijing 100193, Peoples R China. EM xfjiang@ippcaas.cn FU Special Fund for Agro-scientific Research in the Public Interest [201403031, 200903057]; National Natural Science Foundation of China [31000850, 31071641, 31371947]; Beijing Natural Science Foundation [6142017] FX This work was funded by projects under the Special Fund for Agro-scientific Research in the Public Interest (201403031, 200903057), the National Natural Science Foundation of China (No. 31000850, 31071641, 31371947) and the Beijing Natural Science Foundation (6142017). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 2 Z9 3 U1 3 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 27 PY 2015 VL 10 IS 3 AR e0121821 DI 10.1371/journal.pone.0121821 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE8ZT UT WOS:000352133600121 PM 25815767 ER PT J AU Boock, JT King, BC Taw, MN Conrado, RJ Siu, KH Stark, JC Walker, LP Gibson, DM DeLisa, MP AF Boock, Jason T. King, Brian C. Taw, May N. Conrado, Robert J. Siu, Ka-Hei Stark, Jessica C. Walker, Larry P. Gibson, Donna M. DeLisa, Matthew P. TI Repurposing a Bacterial Quality Control Mechanism to Enhance Enzyme Production in Living Cells SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE directed evolution; cellulase; enzyme engineering; protein folding quality control; twin-arginine translocation ID ARGININE TRANSLOCATION PATHWAY; MULTIPLE DISULFIDE BONDS; SMALL-ANGLE SCATTERING; X-RAY-SCATTERING; PROTEIN SOLUBILITY; DIRECTED EVOLUTION; ESCHERICHIA-COLI; GENETIC SELECTION; IN-VIVO; STABILITY AB Heterologous expression of many proteins in bacteria, yeasts, and plants is often limited by low titers of functional protein. To address this problem, we have created a two-tiered directed evolution strategy in Escherichia coli that enables optimization of protein production while maintaining high biological activity. The first tier involves a genetic selection for intracellular protein stability that is based on the folding quality control mechanism inherent to the twin-arginine translocation pathway, while the second is a semi-high-throughput screen for protein function. To demonstrate the utility of this strategy, we isolated variants of the endoglucanase Cel5A, from the plant-pathogenic fungus Fusarium graminearum, whose production was increased by as much as 30-fold over the parental enzyme. This gain in production was attributed to just two amino acid substitutions, and it was isolated after two iterations through the two-tiered approach. There was no significant tradeoff in activity on soluble or insoluble cellulose substrates. Importantly, by combining the folding filter afforded by the twin-arginine translocation quality control mechanism with a function-based screen, we show enrichment for variants with increased protein abundance in a manner that does not compromise catalytic activity, providing a highly soluble parent for engineering of improved or new function. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Boock, Jason T.; Conrado, Robert J.; Siu, Ka-Hei; Stark, Jessica C.; DeLisa, Matthew P.] Cornell Univ, Sch Chem & Biomol Engn, Ithaca, NY 14853 USA. [King, Brian C.; Gibson, Donna M.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Taw, May N.] Cornell Univ, Dept Microbiol, Ithaca, NY 14853 USA. [Walker, Larry P.] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA. [Gibson, Donna M.] ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. RP DeLisa, MP (reprint author), Cornell Univ, Sch Chem & Biomol Engn, Ithaca, NY 14853 USA. EM md255@comell.edu RI King, Brian/K-2021-2016; OI King, Brian/0000-0003-3954-5190; Stark, Jessica/0000-0003-3828-5438; DeLisa, Matthew/0000-0003-3226-1566 FU United States Department of Agriculture National Institute of Food and Agriculture [2009-02202]; Department of Energy Emerging Opportunities Program through the Grant Lakes Bioenergy Research Center [115K555]; National Science Foundation (NSF) [DGE-1045513]; NSF; National Institutes of Health/National Institute of General Medical Sciences under NSF [DMR-1332208]; National Institute of General Medical Sciences, National Institutes of Health [GM-103485] FX We thank Dario Mizrachi and Yujie Chen for helpful discussion of SAXS results. This material is based upon work supported by the United States Department of Agriculture National Institute of Food and Agriculture Grant Number 2009-02202 (to D.M.G., L.P.W., and M.P.D.), the Department of Energy Emerging Opportunities Program Grant 115K555 through the Grant Lakes Bioenergy Research Center (to M.P.D.), and a National Science Foundation (NSF) GK-12 "Grass Roots" Fellowship under Grant Number DGE-1045513 (to J.T.B.). This work is also based upon research conducted at CHESS, which is supported by the NSF and the National Institutes of Health/National Institute of General Medical Sciences under NSF award DMR-1332208, using the macromolecular diffraction at CHESS facility, which is supported by award GM-103485 from the National Institute of General Medical Sciences, National Institutes of Health. NR 50 TC 4 Z9 4 U1 0 U2 9 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2836 EI 1089-8638 J9 J MOL BIOL JI J. Mol. Biol. PD MAR 27 PY 2015 VL 427 IS 6 BP 1451 EP 1463 DI 10.1016/j.jmb.2015.01.003 PN B PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CE4KE UT WOS:000351798700018 PM 25591491 ER PT J AU Twomey, MC Wolfenbarger, SN Woods, JL Gent, DH AF Twomey, Megan C. Wolfenbarger, Sierra N. Woods, Joanna L. Gent, David H. TI Development of Partial Ontogenic Resistance to Powdery Mildew in Hop Cones and Its Management Implications SO PLOS ONE LA English DT Article ID GRAPE BERRIES; PODOSPHAERA-APHANIS; UNCINULA-NECATOR; DOWNY MILDEW; INFECTION; SEVERITY; GROWTH; LEAVES; YIELD AB Knowledge of processes leading to crop damage is central to devising rational approaches to disease management. Multiple experiments established that infection of hop cones by Podosphaera macularis was most severe if inoculation occurred within 15 to 21 days after bloom. This period of infection was associated with the most pronounced reductions in alpha acids, cone color, and accelerated maturation of cones. Susceptibility of cones to powdery mildew decreased progressively after the transition from bloom to cone development, although complete immunity to the disease failed to develop. Maturation of cone tissues was associated with multiple significant affects on the pathogen manifested as reduced germination of conidia, diminished frequency of penetration of bracts, lengthening of the latent period, and decreased sporulation. Cones challenged with P. macularis in juvenile developmental stages also led to greater frequency of colonization by a complex of saprophytic, secondary fungi. Since no developmental stage of cones was immune to powdery mildew, the incidence of powdery mildew continued to increase over time and exceeded 86% by late summer. In field experiments with a moderately susceptible cultivar, the incidence of cones with powdery mildew was statistically similar when fungicide applications were made season-long or targeted only to the juvenile stages of cone development. These studies establish that partial ontogenic resistance develops in hop cones and may influence multiple phases of the infection process and pathogen reproduction. The results further reinforce the concept that the efficacy of a fungicide program may depend largely on timing of a small number of sprays during a relatively brief period of cone development. However in practice, targeting fungicide and other management tactics to periods of enhanced juvenile susceptibility may be complicated by a high degree of asynchrony in cone development and other factors that are situation-dependent. C1 [Twomey, Megan C.; Wolfenbarger, Sierra N.; Woods, Joanna L.; Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gent, David H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR USA. RP Gent, DH (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU US Department of Agriculture Agricultural Research Service Current Research Information System project [5358-21000-035-00]; Hop Research Council; US Department of Agriculture Specialty Crops Research Initiative [SC-2097531] FX Financial support was provided by US Department of Agriculture Agricultural Research Service Current Research Information System project 5358-21000-035-00, Hop Research Council (http://www.hopresearchcouncil.org/), and US Department of Agriculture Specialty Crops Research Initiative project number SC-2097531 (http://www.nifa.usda.gov/fo/specialtycropresearchinitiative.cfm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 2 Z9 2 U1 1 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 26 PY 2015 VL 10 IS 3 AR e0120987 DI 10.1371/journal.pone.0120987 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6QW UT WOS:000356353700072 PM 25811173 ER PT J AU Gaston, LA Kovar, JL AF Gaston, L. A. Kovar, J. L. TI Phytoremediation of High-Phosphorus Soil by Annual Ryegrass and Common Bermudagrass Harvest SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE phosphorus; Agricultural waste; phytoremediation ID WATER-TREATMENT RESIDUALS; BROILER LITTER APPLICATION; COOL-SEASON ANNUALS; NUTRIENT-UPTAKE; POULTRY LITTER; ENRICHED SOILS; SWINE EFFLUENT; AMENDED SOILS; LOLIUM-MULTIFLORUM; ROOT DISTRIBUTION AB Removal of soil phosphorus (P) in crop harvest is a remediation option for soils high in P. This 4-year field-plot study determined P uptake by annual ryegrass (ARG, Lolium multiflorum Lam.) and common bermudagrass (CB, Cynodon dactylon (L.) Pers.) from Ruston soil (fine-loamy, siliceous, thermic Typic Paleudult) enriched in P by five previous annual applications of poultry litter, and related P removed to Bray 2 P in surface (0-15cm) soil. Decreases in surface soil Bray 2 P were largely attributable to uptake. Phosphorus uptake was positively related to Bray 2 P but approached a limit. Mass of P removed in harvest closely approximated the decrease in mass of surface soil Bray 2 P. Maximum Bray 2 P drawdown per harvest (ARG and CB, average) was similar to 3mgkg(-1) at Bray 2 P similar to 300mgkg(-1), generally consistent with measured decreases in Bray 2 P. C1 [Gaston, L. A.] Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. [Kovar, J. L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA USA. RP Gaston, LA (reprint author), Louisiana State Univ, Ctr Agr, 104 Madison B Sturgis Hall, Baton Rouge, LA 70803 USA. EM lagaston@agctr.lsu.edu NR 52 TC 1 Z9 1 U1 5 U2 11 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0010-3624 EI 1532-2416 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD MAR 26 PY 2015 VL 46 IS 6 BP 736 EP 752 DI 10.1080/00103624.2015.1005224 PG 17 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA CF1JK UT WOS:000352301100005 ER PT J AU Hatfield, JL Walthall, CL AF Hatfield, Jerry L. Walthall, Charles L. TI Soil Biological Fertility: Foundation for the Next Revolution in Agriculture? SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE nutrient cycling; AgroBiotic fertilizer; soil biological activity ID MICROBIAL COMMUNITY STRUCTURE; N-MINERALIZATION; NITROGEN MINERALIZATION; QUALITY; PRODUCTIVITY; DEGRADATION; TILLAGE; BIOMASS; INDEXES AB Feeding the world's population in 40years will require improved efficiency in the use of plant nutrients and enhancement of soil resources. Over the past 60years, agricultural production has rapidly increased; however, continued degradation of soil may limit further increases. Improving the soil through enhanced soil biological activity has been proposed as a method of increasing the capacity of the soil to produce crops. Ongoing evaluations of one soil biological fertilizer with a patented process to convert and complex manure into stable finished products (AgroBiotic fertilizers) have been conducted in research plots and producer fields and have shown positive effects on grain yield and potato production. These effects are larger in soils with limited biological activity, suggesting that adding this unique AgroBiotic fertilizer helps restore the biological nutrient cycling in the soil. New developments and innovations that improve nutrient availability and the efficacy of soil biological fertilizers have the potential to help restore degraded soils and improve their production efficiency and capacity to feed the world's population. C1 [Hatfield, Jerry L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA USA. [Walthall, Charles L.] ARS, USDA, Off Natl Programs, Beltsville, MD USA. RP Hatfield, JL (reprint author), 2110 Univ Blvd, Ames, IA 50011 USA. EM jerry.hatfield@ars.usda.gov NR 33 TC 2 Z9 2 U1 4 U2 27 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0010-3624 EI 1532-2416 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD MAR 26 PY 2015 VL 46 IS 6 BP 753 EP 762 DI 10.1080/00103624.2015.1005227 PG 10 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA CF1JK UT WOS:000352301100006 ER PT J AU Eggleston, G Borges, E AF Eggleston, Gillian Borges, Eduardo TI Multiple Applications of Ion Chromatography Oligosaccharide Fingerprint Profiles To Solve a Variety of Sugar and Sugar-Biofuel Industry Problems SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Review DE ion chromatography; pulsed amperometric detection; HPAEC; oligosaccharide fingerprint profiles; sugar cane; sugar beet; sweet sorghum ID ANION-EXCHANGE CHROMATOGRAPHY; PULSED AMPEROMETRIC DETECTION; PROCESSING PROBLEMS; WAVE-FORM; DETERIORATION; PRODUCTS; BEET; ADULTERATION; DEXTRANASES; MANNITOL AB Sugar crops contain a broad variety of carbohydrates used for human consumption and the production of biofuels and bioproducts. Ion chromatography with integrated pulsed amperometric detection (IC-IPAD) can be used to simultaneously detect mono-, di-, and oligosaccharides, oligosaccharide isomers, mannitol, and ethanol in complex matrices from sugar crops. By utilizing a strong NaOH/NaOAc gradient method over 45 min, oligosaccharides of at least 2-12 dp can be detected. Fingerprint IC oligosaccharide profiles are extremely selective, sensitive, and reliable and can detect deterioration product metabolites from as low as 100 colony-forming units/mL lactic acid bacteria. The IC fingerprints can also be used to (i) monitor freeze deterioration, (ii) optimize harvesting methods and cut-to-crush times, (iii) differentiate between white refined sugar from sugar cane and from sugar beets, (iv) verify the activities of carbohydrate enzymes, (v) select yeasts for ethanol fermentations, and (vi) isolate and diagnose infections and processing problems in sugar factories. C1 [Eggleston, Gillian] ARS, Southern Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Borges, Eduardo] Fermentec Ltda, BR-13420640 Piracicaba, Brazil. RP Eggleston, G (reprint author), ARS, Southern Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov NR 43 TC 3 Z9 4 U1 4 U2 39 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 25 PY 2015 VL 63 IS 11 BP 2841 EP 2851 DI 10.1021/jf506370s PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CE6TY UT WOS:000351972300001 PM 25708094 ER PT J AU Krishnan, HB Kim, WS Oehrle, NW Alaswad, AA Baxter, I Wiebold, WJ Nelson, RL AF Krishnan, Hari B. Kim, Won-Seok Oehrle, Nathan W. Alaswad, Alaa A. Baxter, Ivan Wiebold, William J. Nelson, Randall L. TI Introgression of Leginsulin, a Cysteine-Rich Protein, and High-Protein Trait from an Asian Soybean Plant Introduction Genotype into a North American Experimental Soybean Line SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE leginsulin; soybean; sulfur; cysteine; methionine ID BASIC 7S GLOBULIN; NUTRITIONAL QUALITY; INHIBITOR ACTIVITY; TRYPSIN-INHIBITOR; GLYCINE-MAX; SEED; IDENTIFICATION; METHIONINE; YIELD; GENE AB Soybean is an important protein source for both humans and animals. However, soybean proteins are relatively poor in the sulfur-containing amino acids, cysteine and methionine. Improving the content of endogenous proteins rich in sulfur-containing amino acids could enhance the nutritive value of soybean meal. Leginsulin, a cysteine-rich peptide, predominantly accumulates in Asian soybean accessions but not in most North American cultivars. By screening diverse soybean accessions from the USDA Soybean Germplasm Collection, we were able to identify one plant introduction, PI 427138, as a high-protein line with relatively high amounts of both elemental sulfur and leginsulin. We introgressed these desirable traits from PI 427138 into an experimental line with the aim of improving the overall protein content and quality of seed proteins. Biochemical characterization of inbred progenies from the cross of LD00-3309 with PI 427138 grown at six locations revealed stable ingression of high protein, high elemental sulfur, and high leginsulin accumulation. Comparison of soybean seed proteins resolved by high-resolution 2-D gel electrophoresis in combination with Delta2D image analysis software revealed preferential accumulation of a few glycinin subunits contributed to the increased protein content in the introgressed lines. Amino acid analysis revealed that even though the leginsulin introgressed lines had higher protein, leginsulin, and elemental sulfur, the overall concentration of sulfur-containing amino acids was not significantly altered when compared with the parental lines. The experimental soybean lines developed during this study (Leg-3, Leg-7, and Leg-8) lack A5, A4, and B3 glycinin subunits and could be utilized in breeding programs to develop high-quality tofu cultivars. C1 [Krishnan, Hari B.; Oehrle, Nathan W.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. [Kim, Won-Seok; Alaswad, Alaa A.; Wiebold, William J.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. [Alaswad, Alaa A.] King Abdulaziz Univ, Jeddah, Saudi Arabia. [Baxter, Ivan] ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, USDA, St Louis, MO 63132 USA. [Nelson, Randall L.] Univ Illinois, Soybean Maize Germplasm Pathol & Genet Res Unit, ARS, USDA,Dept Crop Sci, Urbana, IL 61801 USA. RP Krishnan, HB (reprint author), Univ Missouri, Plant Genet Res Unit, USDA ARS, 108 Curtis Hall, Columbia, MO 65211 USA. EM Hari.Krishnan@ars.usda.gov RI Baxter, Ivan/A-1052-2009 OI Baxter, Ivan/0000-0001-6680-1722 NR 39 TC 0 Z9 0 U1 1 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 25 PY 2015 VL 63 IS 11 BP 2862 EP 2869 DI 10.1021/jf505202z PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CE6TY UT WOS:000351972300003 PM 25756929 ER PT J AU Chynoweth, MW Lepczyk, CA Litton, CM Hess, SC Kellner, JR Cordell, S AF Chynoweth, Mark W. Lepczyk, Christopher A. Litton, Creighton M. Hess, Steven C. Kellner, James R. Cordell, Susan TI Home Range Use and Movement Patterns of Non-Native Feral Goats in a Tropical Island Montane Dry Landscape SO PLOS ONE LA English DT Article ID DIFFERENCE VEGETATION INDEX; CAPRA-HIRCUS; SOCIAL SEGREGATION; ANIMAL ECOLOGY; DYNAMICS; UNGULATE; GPS; ECOSYSTEMS; RAINFALL; HABITAT AB Advances in wildlife telemetry and remote sensing technology facilitate studies of broad-scale movements of ungulates in relation to phenological shifts in vegetation. In tropical island dry landscapes, home range use and movements of non-native feral goats (Capra hircus) are largely unknown, yet this information is important to help guide the conservation and restoration of some of the world's most critically endangered ecosystems. We hypothesized that feral goats would respond to resource pulses in vegetation by traveling to areas of recent green-up. To address this hypothesis, we fitted six male and seven female feral goats with Global Positioning System (GPS) collars equipped with an Argos satellite upload link to examine goat movements in relation to the plant phenology using the Normalized Difference Vegetation Index (NDVI). Movement patterns of 50% of males and 40% of females suggested conditional movement between non-overlapping home ranges throughout the year. A shift in NDVI values corresponded with movement between primary and secondary ranges of goats that exhibited long-distance movement, suggesting that vegetation phenology as captured by NDVI is a good indicator of the habitat and movement patterns of feral goats in tropical island dry landscapes. In the context of conservation and restoration of tropical island landscapes, the results of our study identify how non-native feral goats use resources across a broad landscape to sustain their populations and facilitate invasion of native plant communities. C1 [Chynoweth, Mark W.; Lepczyk, Christopher A.; Litton, Creighton M.] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Hess, Steven C.] US Geol Survey, Pacific Isl Ecosyst Res Ctr, Hawail Natl Pk, HI USA. [Kellner, James R.] Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA. [Cordell, Susan] US Forest Serv, USDA, Inst Pacific Islands Forestry, Hilo, HI USA. RP Chynoweth, MW (reprint author), Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. EM chynoweth.mark@gmail.com FU National Science Foundation Graduate Research Fellowship [2010094953]; USDA Forest Service, Pacific Southwest Research Station, Institute of Pacific Islands Forestry Research Joint Venture [08-JV-11272177-074]; Strategic Environmental Research and Development Program [RC-1645]; W. T. Yoshimoto Foundation Endowed Fellowship in Animal Wildlife Conservation Biology; Invasive Species Program of USGS FX Funding was provided by the National Science Foundation Graduate Research Fellowship under Grant #2010094953 to MWC; USDA Forest Service, Pacific Southwest Research Station, Institute of Pacific Islands Forestry Research Joint Venture 08-JV-11272177-074 to CML; Strategic Environmental Research and Development Program RC-1645 to SC; W. T. Yoshimoto Foundation Endowed Fellowship in Animal Wildlife Conservation Biology to MWC. Support for SH was provided by the Invasive Species Program of USGS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 0 Z9 0 U1 3 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 25 PY 2015 VL 10 IS 3 AR e0119231 DI 10.1371/journal.pone.0119231 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE5MO UT WOS:000351880000023 PM 25807275 ER PT J AU Hake, S Ross-Ibarra, J AF Hake, Sarah Ross-Ibarra, Jeffrey TI Genetic, evolutionary and plant breeding insights from the domestication of maize SO ELIFE LA English DT Article ID ASSOCIATION MAPPING POPULATION; GENOME-WIDE ASSOCIATION; ZEA-MAYS; MORPHOLOGICAL DIFFERENCES; QUANTITATIVE RESISTANCE; LEAF-BLIGHT; TEOSINTE; ARCHITECTURE; LANDRACES; ORIGIN AB The natural history of maize began nine thousand years ago when Mexican farmers started to collect the seeds of the wild grass, teosinte. Invaluable as a food source, maize permeated Mexican culture and religion. Its domestication eventually led to its adoption as a model organism, aided in large part by its large chromosomes, ease of pollination and growing agricultural importance. Genome comparisons between varieties of maize, teosinte and other grasses are beginning to identify the genes responsible for the domestication of modern maize and are also providing ideas for the breeding of more hardy varieties. C1 [Hake, Sarah] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Hake, Sarah] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Ross-Ibarra, Jeffrey] Univ Calif Davis, Ctr Populat Biol, Dept Plant Sci, Davis, CA 95616 USA. [Ross-Ibarra, Jeffrey] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. RP Hake, S (reprint author), USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. EM hake@berkeley.edu OI Ross-Ibarra, Jeffrey/0000-0003-1656-4954 FU U.S. Department of Agriculture (USDA) [CRIS 5335-21000-035]; National Science Foundation (NSF) [1238014] FX U.S. Department of Agriculture (USDA) CRIS 5335-21000-035 Sarah Hake; National Science Foundation (NSF) Integrative Organismal Systems 1238014 Jeffrey Ross-Ibarra NR 50 TC 7 Z9 7 U1 7 U2 50 PU ELIFE SCIENCES PUBLICATIONS LTD PI CAMBRIDGE PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND SN 2050-084X J9 ELIFE JI eLife PD MAR 25 PY 2015 VL 4 AR e05861 DI 10.7554/eLife.05861 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CE5JA UT WOS:000351867100010 ER PT J AU Andersson, L Archibald, AL Bottema, CD Brauning, R Burgess, SC Burt, DW Casas, E Cheng, HH Clarke, L Couldrey, C Dalrymple, BP Elsik, CG Foissac, S Giuffra, E Groenen, MA Hayes, BJ Huang, LS Khatib, H Kijas, JW Kim, H Lunney, JK McCarthy, FM McEwan, JC Moore, S Nanduri, B Notredame, C Palti, Y Plastow, GS Reecy, JM Rohrer, GA Sarropoulou, E Schmidt, CJ Silverstein, J Tellam, RL Tixier-Boichard, M Tosser-Klopp, G Tuggle, CK Vilkki, J White, SN Zhao, SH Zhou, H AF Andersson, Leif Archibald, Alan L. Bottema, Cynthia D. Brauning, Rudiger Burgess, Shane C. Burt, Dave W. Casas, Eduardo Cheng, Hans H. Clarke, Laura Couldrey, Christine Dalrymple, Brian P. Elsik, Christine G. Foissac, Sylvain Giuffra, Elisabetta Groenen, Martien A. Hayes, Ben J. Huang, LuSheng S. Khatib, Hassan Kijas, James W. Kim, Heebal Lunney, Joan K. McCarthy, Fiona M. McEwan, John C. Moore, Stephen Nanduri, Bindu Notredame, Cedric Palti, Yniv Plastow, Graham S. Reecy, James M. Rohrer, Gary A. Sarropoulou, Elena Schmidt, Carl J. Silverstein, Jeffrey Tellam, Ross L. Tixier-Boichard, Michele Tosser-Klopp, Gwenola Tuggle, Christopher K. Vilkki, Johanna White, Stephen N. Zhao, Shuhong Zhou, Huaijun CA FAANG Consortium TI Coordinated international action to accelerate genome-to-phenome with FAANG, the Functional Annotation of Animal Genomes project SO GENOME BIOLOGY LA English DT Article ID ENCODE PROJECT; COMPLEX TRAITS; DNA ELEMENTS; MOUSE GENOME; CHROMATIN; EVOLUTION; SELECTION; REVEALS; CATTLE; DOMESTICATION AB We describe the organization of a nascent international effort, the Functional Annotation of Animal Genomes (FAANG) project, whose aim is to produce comprehensive maps of functional elements in the genomes of domesticated animal species. C1 [Andersson, Leif] Uppsala Univ, Dept Med Biochem & Microbiol, Sci Life Lab Uppsala, S-75123 Uppsala, Sweden. [Andersson, Leif] Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden. [Archibald, Alan L.; Burt, Dave W.] Univ Edinburgh, Roslin Inst, Edinburgh EH25 9RG, Midlothian, Scotland. [Archibald, Alan L.] Univ Edinburgh, Royal Dick Sch Vet Studies, Edinburgh EH25 9RG, Midlothian, Scotland. [Bottema, Cynthia D.] Univ Adelaide, Sch Anim & Vet Sci, Roseworthy, SA 5371, Australia. [Brauning, Rudiger] AgResearch, Invermay Agr Ctr, Mosgiel 9053, New Zealand. [Burgess, Shane C.] Univ Arizona, Coll Agr & Life Sci, Tucson, AZ 85719 USA. [Casas, Eduardo] USDA, Natl Anim Dis Ctr, Agr Res Serv, Ames, IA 50010 USA. [Cheng, Hans H.] USDA, ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Clarke, Laura] European Bioinformat Inst, European Mol Biol Lab, Cambridge CB10 1SD, England. [Couldrey, Christine] Livestock Improvement Corp, Hamilton 3284, New Zealand. [Dalrymple, Brian P.; Kijas, James W.] CSIRO, Agr Flagship, St Lucia, Qld 4067, Australia. [Elsik, Christine G.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Foissac, Sylvain; Tosser-Klopp, Gwenola] French Natl Inst Agr Res INRA, UMR Genet Physiol & Syst Elevage GenPhySE 1388, F-31326 Castanet Tolosan, France. [Giuffra, Elisabetta; Tixier-Boichard, Michele] French Natl Inst Agr Res INRA, UMR GABI 1313, F-78352 Jouy en Josas, France. [Groenen, Martien A.] Wageningen Univ, Anim Breeding & Genom Ctr, NL-6708 PB Wageningen, Netherlands. [Hayes, Ben J.] Dept Environm & Primary Ind Victoria, Biosci Res Div, Bundoora, Vic 3083, Australia. [Hayes, Ben J.] Dairy Futures Cooperat Res Ctr, Bundoora, Vic 3083, Australia. [Hayes, Ben J.] La Trobe Univ, Bundoora, Vic 3086, Australia. [Huang, LuSheng S.] Jiangxi Agr Univ, Key Lab Anim Biotechnol Jiangxi Prov, Nanchang 330029, Jiangxi, Peoples R China. [Huang, LuSheng S.] Jiangxi Agr Univ, Minist Agr China, Nanchang 330029, Jiangxi, Peoples R China. [Khatib, Hassan] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA. [Kim, Heebal] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea. [Lunney, Joan K.] USDA, Anim Parasit Dis Lab, ARS, Beltsville, MD 20705 USA. [McCarthy, Fiona M.] Univ Arizona, Schl Anim & Comparat Biomed Sci, Tucson, AZ 85721 USA. [McEwan, John C.] AgResearch, Anim Product Grp, Mosgiel 9053, New Zealand. [Moore, Stephen] Univ Queensland, Ctr Anim Sci Queensland Alliance Agr & Food Innov, St Lucia, Qld 4067, Australia. [Nanduri, Bindu] Mississippi State Univ, Dept Basic Sci, Coll Vet Med, Mississippi State, MS USA. [Nanduri, Bindu] Mississippi State Univ, Inst Genom Biocomput & Biotechnol, Mississippi State, MS USA. [Notredame, Cedric] Ctr Genom Regulat CRG, Comparat Bioinformat, Barcelona 08003, Spain. [Palti, Yniv] USDA, Natl Ctr Cool & Cold Water Aquaculture, ARS, Kearneysville, WV 25430 USA. [Plastow, Graham S.] Univ Alberta, Dept Agr Food & Nutr Sci, Livestock Gentec Ctr, Edmonton, AB T6G 2C8, Canada. [Reecy, James M.; Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Rohrer, Gary A.] USDA, US Meat Anim Res Ctr, ARS, Clay Ctr, Clay Ctr, NE 68933 USA. [Sarropoulou, Elena] Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture, Iraklion 71003, Greece. [Schmidt, Carl J.] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. [Silverstein, Jeffrey] USDA, Anim Prod & Protect, Agr Res Serv Aquaculture, Beltsville, MD 20705 USA. [Tellam, Ross L.] CSIRO Agr, Commonwealth Sci & Ind Res Org, St Lucia, Qld 4067, Australia. [Vilkki, Johanna] Nat Resources Inst Finland, Green Technol, Jokioinen 31600, Finland. [White, Stephen N.] USDA, Anim Dis Res Unit, ARS, Pullman, WA 99164 USA. [White, Stephen N.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Zhao, Shuhong] Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ China, Wuhan 430070, Peoples R China. [Zhou, Huaijun] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. RP Giuffra, E (reprint author), French Natl Inst Agr Res INRA, UMR GABI 1313, F-78352 Jouy En Josas, France. EM elisabetta.giuffra@jouy.inra.fr; cktuggle@iastate.edu RI Dalrymple, Brian/F-8721-2010; Tellam, Ross/H-2798-2013; Kijas, James/A-4656-2011; Groenen, Martien/D-8408-2012; Hayes, Ben/L-3308-2016; McEwan, John/A-1143-2008; Elsik, Christine/C-4120-2017; Giuffra, Elisabetta/J-8080-2014; OI Clarke, Laura/0000-0002-5989-6898; Archibald, Alan/0000-0001-9213-1830; TOSSER-KLOPP, GWENOLA/0000-0003-0550-4673; Kim, Heebal/0000-0003-3064-1303; Dalrymple, Brian/0000-0003-3891-5233; Tellam, Ross/0000-0003-2353-1640; Kijas, James/0000-0002-1417-8278; Groenen, Martien/0000-0003-0484-4545; Hayes, Ben/0000-0002-5606-3970; McEwan, John/0000-0003-4801-6207; Elsik, Christine/0000-0002-4248-7713; Brauning, Rudiger/0000-0001-5068-9166; Foissac, Sylvain/0000-0002-2631-5356; Schmidt, Carl/0000-0002-8386-4781 FU NIGMS NIH HHS [P20 GM103646] NR 53 TC 28 Z9 29 U1 6 U2 27 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1465-6906 EI 1474-760X J9 GENOME BIOL JI Genome Biol. PD MAR 25 PY 2015 VL 16 AR UNSP 57 DI 10.1186/s13059-015-0622-4 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CE4UL UT WOS:000351825600001 PM 25854118 ER PT J AU Zhao, Y Chang, YS Chen, P AF Zhao, Yang Chang, Yuan-Shiun Chen, Pei TI Differentiation of Aurantii Fructus Immaturus from Poniciri Trifoliatae Fructus Immaturus using flow-injection mass spectrometric (FIMS) metabolic fingerprinting method combined with chemometrics SO JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS LA English DT Article DE FIMS; Metabolic; Fingerprint; Chemometrics; Bitter orange ID FLAVONOID O-DIGLYCOSIDES; ELECTROSPRAY-IONIZATION; MULTIVARIATE-ANALYSIS; CELL-LINES; CITRUS; SAMPLES; POLYMETHOXYFLAVONES; IDENTIFICATION; CONSTITUENTS; NOBILETIN AB A flow-injection mass spectrometric metabolic fingerprinting method in combination with chemometrics was used to differentiate Aurantii Fructus Immaturus from its counterfeit Poniciri Trifoliatae Fructus Immaturus. Flow-injection mass spectrometric (FIMS) fingerprints of 9 Aurantii Fructus Immaturus samples and 12 Poniciri Trifoliatae Fructus Immaturus samples were acquired and analyzed using principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA). The authentic herbs were differentiated from their counterfeits easily. Eight characteristic components which were responsible for the differences between the samples were tentatively identified. Furthermore, three out of the eight components, naringin, hesperidin, and neohesperidin, were quantified. The results are useful to help identify the authenticity of Aurantii Fructus Immaturus. Published by Elsevier B.V. C1 [Zhao, Yang; Chen, Pei] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Chang, Yuan-Shiun] China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Coll Pharm, Taichung 40402, Taiwan. RP Chen, P (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Bldg 161,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov RI ZHAO, YANG/O-7920-2014 FU NIH HHS [Y01 OD001298-01] NR 29 TC 2 Z9 2 U1 3 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0731-7085 EI 1873-264X J9 J PHARMACEUT BIOMED JI J. Pharm. Biomed. Anal. PD MAR 25 PY 2015 VL 107 BP 251 EP 257 DI 10.1016/j.jpba.2014.12.035 PG 7 WC Chemistry, Analytical; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA CD5GV UT WOS:000351116900033 PM 25622204 ER PT J AU Klotz, JL Smith, DL AF Klotz, James L. Smith, Darrin L. TI Recent investigations of ergot alkaloids incorporated into plant and/or animal systems SO FRONTIERS IN CHEMISTRY LA English DT Editorial Material DE ergot alkaloids; livestock; animal systems; plant systems; fungus ID INFECTED PERENNIAL RYEGRASS; TALL FESCUE; ERGOPEPTINE ALKALOIDS; MASS-SPECTROMETRY; EPICHLOE; PERFORMANCE; ENDOPHYTES; RESPONSES; LIVESTOCK; EXPOSURE C1 [Klotz, James L.] ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY USA. [Smith, Darrin L.] Eastern Kentucky Univ, Dept Chem, Richmond, KY 40475 USA. RP Klotz, JL (reprint author), ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY USA. EM james.klotz@ars.usda.gov NR 32 TC 0 Z9 0 U1 3 U2 10 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND EI 2296-2646 J9 FRONT CHEM JI Front. Chem. PD MAR 25 PY 2015 VL 3 AR 23 DI 10.3389/fchem.2015.00023 PG 3 WC Chemistry, Multidisciplinary SC Chemistry GA DI2VJ UT WOS:000373356800001 PM 25859540 ER PT J AU Kim, ES Sonstegard, TS da Silva, MVGB Gasbarre, LC Van Tassell, CP AF Kim, Eui-Soo Sonstegard, Tad S. da Silva, Marcos V. G. B. Gasbarre, Louis C. Van Tassell, Curtis P. TI Genome-Wide Scan of Gastrointestinal Nematode Resistance in Closed Angus Population Selected for Minimized Influence of MHC SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; NATURAL-SELECTION; HAEMONCHUS-CONTORTUS; GENETIC-CONTROL; SHEEP; INFECTION; CATTLE; QUANTIFICATION; SUSCEPTIBILITY AB Genetic markers associated with parasite indicator traits are ideal targets for study of marker assisted selection aimed at controlling infections that reduce herd use of anthelminthics. For this study, we collected gastrointestinal (GI) nematode fecal egg count (FEC) data from post-weaning animals of an Angus resource population challenged to a 26 week natural exposure on pasture. In all, data from 487 animals was collected over a 16 year period between 1992 and 2007, most of which were selected for a specific DRB1 allele to reduce the influence of potential allelic variant effects of the MHC locus. A genome-wide association study (GWAS) based on BovineSNP50 genotypes revealed six genomic regions located on bovine Chromosomes 3, 5, 8, 15 and 27; which were significantly associated (-log(10) p=4.3) with Box-Cox transformed mean FEC (BC-MFEC). DAVID analysis of the genes within the significant genomic regions suggested a correlation between our results and annotation for genes involved in inflammatory response to infection. Furthermore, ROH and selection signature analyses provided strong evidence that the genomic regions associated BC-MFEC have not been affected by local autozygosity or recent experimental selection. These findings provide useful information for parasite resistance prediction for young grazing cattle and suggest new candidate gene targets for development of disease-modifying therapies or future studies of host response to GI parasite infection. C1 [Kim, Eui-Soo; Sonstegard, Tad S.; Gasbarre, Louis C.; Van Tassell, Curtis P.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [da Silva, Marcos V. G. B.] Empresa Brasileira Pesquisa Agr, Embrapa Gado Leite, Juiz De Fora, Brazil. RP Sonstegard, TS (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM tad.sonstegard@ars.usda.gov FU USDA-ARS [1265-31000-093-00, 104-00]; TS trust agreement with Merial-Igenity [1265-32000-084-01T]; Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008196] FX This work was supported in part by Projects 1265-31000-093-00 and 104-00 from USDA-ARS, and a TS trust agreement with Merial-Igenity (1265-32000-084-01T). TSS, LCG and CVT received the funding. ESK was partially supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ008196), Rural Development Administration, Republic of Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 62 TC 1 Z9 1 U1 1 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 24 PY 2015 VL 10 IS 3 AR e0119380 DI 10.1371/journal.pone.0119380 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH2XV UT WOS:000353889600024 PM 25803687 ER PT J AU Kim, HU Chen, GQ AF Kim, Hyun Uk Chen, Grace Qianhong TI Identification of hydroxy fatty acid and triacylglycerol metabolism-related genes in lesquerella through seed transcriptome analysis SO BMC GENOMICS LA English DT Article DE Hydroxy fatty acid; Lesquerella; Physaria fendleri; Seed; Transcriptome; Triacylglycerol; Gene expression; Quantitative polymerase chain reaction ID DIACYLGLYCEROL ACYLTRANSFERASE; TRANSGENIC ARABIDOPSIS; ENZYMATIC-SYNTHESIS; FENDLERI SEED; OIL CONTENT; MICROSOMAL PREPARATIONS; PHYSARIA BRASSICACEAE; OLEATE 12-HYDROXYLASE; CONDENSING ENZYME; RICINOLEIC ACID AB Background: Castor oil is the only commercial source of hydroxy fatty acid that has industrial value. The production of castor oil is hampered by the presence of the toxin ricin in its seed. Lesquerella seed also accumulates hydroxy fatty acid and is free of ricin, and thus it is being developed as a new crop for hydroxy fatty acid production. A high-throughput, large-scale sequencing of transcripts from developing lesquerella seeds was carried out by 454 pyrosequencing to generate a database for quality improvement of seed oil and other agronomic traits. Deep mining and characterization of acyl-lipid genes were conducted to uncover candidate genes for further studies of mechanisms underlying hydroxy fatty acid and seed oil synthesis. Results: A total of 651 megabases of raw sequences from an mRNA sample of developing seeds was acquired. Bioinformatic analysis of these sequences revealed 59,914 transcripts representing 26,995 unique genes that include nearly all known seed expressed genes. Based on sequence similarity with known plant proteins, about 74% (19,861) genes matched with annotated coding genes. Among them, 95% (18,868) showed highest sequence homology with Arabidopsis genes, which will allow translation of genomics and genetics findings from Arabidopsis to lesquerella. Using Arabidopsis acyl-lipid genes as queries, we searched the transcriptome assembly and identified 615 lesquerella genes involved in all known pathways of acyl-lipid metabolism. Further deep mining the transcriptome assembly led to identification of almost all lesquerella genes involved in fatty acid and triacylglycerol synthesis. Moreover, we characterized the spatial and temporal expression profiles of 15 key genes using the quantitative PCR assay. Conclusions: We have built a lesquerella seed transcriptome that provides a valuable reference in addition to the castor database for discovering genes involved in the synthesis of triacylglycerols enriched with hydroxy fatty acids. The information obtained from data mining and gene expression profiling will provide a resource not only for the study of hydroxy fatty acid metabolism, but also for the biotechnological production of hydroxy fatty acids in existing oilseed crops. C1 [Kim, Hyun Uk] Natl Acad Agr Sci Rural Dev Adm, Dept Agr Biotechnol, Jeonju 560500, South Korea. [Chen, Grace Qianhong] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Kim, HU (reprint author), Natl Acad Agr Sci Rural Dev Adm, Dept Agr Biotechnol, Jeonju 560500, South Korea. EM hukim64@korea.kr; grace.chen@ars.usda.gov OI KIM, HYUN UK/0000-0002-4566-3057 FU National Academy of Agricultural Science [PJ01007505]; International Cooperative Research Project Program of the National Academy of Agricultural Science [PJ00855602]; "Next-Generation BioGreen 21 Program (SSAC)" of the Rural development Administration (RDA), Republic of Korea [PJ01108101]; US Department of Agriculture-Agricultural Research Service-Current Research Information System Project [5325-21410-020-00D]; USDA Trust Fund Cooperative Agreement [58 0212 9 036 F]; RDA FX This study was conducted with support from the National Academy of Agricultural Science (project no. PJ01007505) and International Cooperative Research Project Program (Project No. PJ00855602) of the National Academy of Agricultural Science and "Next-Generation BioGreen 21 Program (SSAC, Project No. PJ01108101)" of the Rural development Administration (RDA), Republic of Korea, from the US Department of Agriculture-Agricultural Research Service-Current Research Information System Project 5325-21410-020-00D, and from the USDA Trust Fund Cooperative Agreement with RDA (Agreement number: 58 0212 9 036 F). The authors wish to thank Ann Blechl and Mark Smith for critical reading of the manuscript, and Kumiko Johnson for assisting qPCR analysis. Thanks also go to Dr. David Dierig and Dr. Mark Cruz for providing the cover photo of leaquerella grown in a research field of US Department of Agriculture. USDA is an equal opportunity provider and employer. Mention of a specific product name by the United States Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 92 TC 4 Z9 4 U1 4 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 24 PY 2015 VL 16 AR 230 DI 10.1186/s12864-015-1413-8 PG 21 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CE7CG UT WOS:000351994000001 PM 25881190 ER PT J AU Rodgers-Melnick, E Bradbury, PJ Elshire, RJ Glaubitz, JC Acharya, CB Mitchell, SE Li, CH Li, YX Buckler, ES AF Rodgers-Melnick, Eli Bradbury, Peter J. Elshire, Robert J. Glaubitz, Jeffrey C. Acharya, Charlotte B. Mitchell, Sharon E. Li, Chunhui Li, Yongxiang Buckler, Edward S. TI Recombination in diverse maize is stable, predictable, and associated with genetic load SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE recombination; maize; genetic load; deleterious mutations; methylation ID ZEA-MAYS L; DNA METHYLATION; MEIOTIC RECOMBINATION; ARABIDOPSIS; POPULATIONS; EVOLUTION; CONVERSION; LANDSCAPE; DOMESTICATION; FREQUENCIES AB Among the fundamental evolutionary forces, recombination arguably has the largest impact on the practical work of plant breeders. Varying over 1,000-fold across the maize genome, the local meiotic recombination rate limits the resolving power of quantitative trait mapping and the precision of favorable allele introgression. The consequences of low recombination also theoretically extend to the species-wide scale by decreasing the power of selection relative to genetic drift, and thereby hindering the purging of deleterious mutations. In this study, we used genotyping-by-sequencing (GBS) to identify 136,000 recombination breakpoints at high resolution within US and Chinese maize nested association mapping populations. We find that the pattern of cross-overs is highly predictable on the broad scale, following the distribution of gene density and CpG methylation. Several large inversions also suppress recombination in distinct regions of several families. We also identify recombination hotspots ranging in size from 1 kb to 30 kb. We find these hotspots to be historically stable and, compared with similar regions with low recombination, to have strongly differentiated patterns of DNA methylation and GC content. We also provide evidence for the historical action of GC-biased gene conversion in recombination hotspots. Finally, using genomic evolutionary rate profiling (GERP) to identify putative deleterious polymorphisms, we find evidence for reduced genetic load in hotspot regions, a phenomenon that may have considerable practical importance for breeding programs worldwide. C1 [Rodgers-Melnick, Eli; Bradbury, Peter J.; Elshire, Robert J.; Glaubitz, Jeffrey C.; Acharya, Charlotte B.; Mitchell, Sharon E.; Buckler, Edward S.] Cornell Univ, Inst Genom Div, Ithaca, NY 14853 USA. [Bradbury, Peter J.; Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. [Li, Chunhui; Li, Yongxiang] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China. RP Rodgers-Melnick, E (reprint author), Cornell Univ, Inst Genom Div, Ithaca, NY 14853 USA. EM er432@cornell.edu; pjb39@cornell.edu OI Buckler, Edward/0000-0002-3100-371X; Elshire, Robert/0000-0003-1753-6920 FU National Science Foundation [0820619, 1238014]; Ministry of Science and Technology of China [2011DFA30450]; US Department of Agriculture-Agricultural Research Service FX We thank Alex Lipka and Jeff Ross-Ibarra for helpful comments on several of the analyses during the preparation of this manuscript. We also thank three anonymous reviewers for their insightful comments. This work was supported by National Science Foundation Grants 0820619 and 1238014, Ministry of Science and Technology of China Grant 2011DFA30450, and the US Department of Agriculture-Agricultural Research Service. NR 45 TC 26 Z9 26 U1 4 U2 43 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 24 PY 2015 VL 112 IS 12 BP 3823 EP 3828 DI 10.1073/pnas.1413864112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE0EJ UT WOS:000351477000064 PM 25775595 ER PT J AU Czemmel, S Galarneau, ER Travadon, R McElrone, AJ Cramer, GR Baumgartner, K AF Czemmel, Stefan Galarneau, Erin R. Travadon, Renaud McElrone, Andrew J. Cramer, Grant R. Baumgartner, Kendra TI Genes Expressed in Grapevine Leaves Reveal Latent Wood Infection by the Fungal Pathogen Neofusicoccum parvum SO PLOS ONE LA English DT Article ID CV CABERNET-SAUVIGNON; VITIS-VINIFERA; EUTYPA-LATA; ESCA DISEASE; TRUNK DISEASES; DIEBACK; PLANT; BOTRYOSPHAERIACEAE; TRANSCRIPTOME; RESPONSES AB Some pathogenic species of the Botryosphaeriaceae have a latent phase, colonizing woody tissues while perennial hosts show no apparent symptoms until conditions for disease development become favorable. Detection of these pathogens is often limited to the later pathogenic phase. The latent phase is poorly characterized, despite the need for non-destructive detection tools and effective quarantine strategies, which would benefit from identification of host-based markers in leaves. Neofusicoccum parvum infects the wood of grapevines and other horticultural crops, killing the fruit-bearing shoots. We used light microscopy and high-resolution computed tomography (HRCT) to examine the spatiotemporal relationship between pathogen colonization and anatomical changes in stem sections. To identify differentially-expressed grape genes, leaves from inoculated and noninoculated plants were examined using RNA-Seq. The latent phase occurred between 0 and 1.5 months post-inoculation (MPI), during which time the pathogen did not spread significantly beyond the inoculation site nor were there differences in lesion lengths between inoculated and non-inoculated plants. The pathogenic phase occurred between 1.5 and 2 MPI, when recovery beyond the inoculation site increased and lesion lengths of inoculated plants tripled. By 2 MPI, inoculated plants also had decreased starch content in xylem fibers and rays, and increased levels of gel-occluded xylem vessels, the latter of which HRCT revealed at a higher frequency than microscopy. RNA-Seq and screening of 21 grape expression datasets identified 20 candidate genes that were transcriptionally-activated by infection during the latent phase, and confirmed that the four best candidates (galactinol synthase, abscisic acid-induced wheat plasma membrane polypeptide-19 ortholog, embryonic cell protein 63, BURP domain-containing protein) were not affected by a range of common foliar and wood pathogens or abiotic stresses. Assuming such host responses are consistent among cultivars, and do not cross react with other trunk/foliar pathogens, these grape genes may serve as host-based markers of the latent phase of N. parvum infection. C1 [Czemmel, Stefan; Cramer, Grant R.] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA. [Galarneau, Erin R.; McElrone, Andrew J.; Baumgartner, Kendra] USDA ARS, Davis, CA 94710 USA. [Travadon, Renaud] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Baumgartner, K (reprint author), USDA ARS, Davis, CA 94710 USA. EM kbaumgartner@ucdavis.edu FU United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative [2012-51181-19954] FX This research was funded by grant 2012-51181-19954 to Baumgartner and Cramer from the United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 5 Z9 5 U1 1 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 23 PY 2015 VL 10 IS 3 AR e0121828 DI 10.1371/journal.pone.0121828 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE6ZS UT WOS:000351987300294 PM 25798871 ER PT J AU Thumbi, SM Njenga, MK Marsh, TL Noh, S Otiang, E Munyua, P Ochieng, L Ogola, E Yoder, J Audi, A Montgomery, JM Bigogo, G Breiman, RF Palmer, GH McElwain, TF AF Thumbi, S. M. Njenga, M. Kariuki Marsh, Thomas L. Noh, Susan Otiang, Elkanah Munyua, Peninah Ochieng, Linus Ogola, Eric Yoder, Jonathan Audi, Allan Montgomery, Joel M. Bigogo, Godfrey Breiman, Robert F. Palmer, Guy H. McElwain, Terry F. TI Linking Human Health and Livestock Health: A "One-Health" Platform for Integrated Analysis of Human Health, Livestock Health, and Economic Welfare in Livestock Dependent Communities SO PLOS ONE LA English DT Article ID RURAL WESTERN KENYA; DEMOGRAPHIC SURVEILLANCE; DEVELOPING-COUNTRIES; NUTRITION; DISEASES; DETERMINANTS; MALNUTRITION; MORBIDITY; IMMUNITY; CHILDREN AB Background For most rural households in sub-Saharan Africa, healthy livestock play a key role in averting the burden associated with zoonotic diseases, and in meeting household nutritional and socio-economic needs. However, there is limited understanding of the complex nutritional, socio-economic, and zoonotic pathways that link livestock health to human health and welfare. Here we describe a platform for integrated human health, animal health and economic welfare analysis designed to address this challenge. We provide baseline epidemiological data on disease syndromes in humans and the animals they keep, and provide examples of relationships between human health, animal health and household socio-economic status. Method We designed a study to obtain syndromic disease data in animals along with economic and behavioral information for 1500 rural households in Western Kenya already participating in a human syndromic disease surveillance study. Data collection started in February 2013, and each household is visited bi-weekly and data on four human syndromes (fever, jaundice, diarrhea and respiratory illness) and nine animal syndromes (death, respiratory, reproductive, musculoskeletal, nervous, urogenital, digestive, udder disorders, and skin disorders in cattle, sheep, goats and chickens) are collected. Additionally, data from a comprehensive socio-economic survey is collected every 3 months in each of the study households. Findings Data from the first year of study showed 93% of the households owned at least one form of livestock (55%, 19%, 41% and 88% own cattle, sheep, goats and chickens respectively). Digestive disorders, mainly diarrhea episodes, were the most common syndromes observed in cattle, goats and sheep, accounting for 56% of all livestock syndromes, followed by respiratory illnesses (18%). In humans, respiratory illnesses accounted for 54% of all illnesses reported, followed by acute febrile illnesses (40%) and diarrhea illnesses (5%). While controlling for household size, the incidence of human illness increased 1.31-fold for every 10 cases of animal illness or death observed (95% CI 1.16-1.49). Access and utilization of animal source foods such as milk and eggs were positively associated with the number of cattle and chickens owned by the household. Additionally, health care seeking was correlated with household incomes and wealth, which were in turn correlated with livestock herd size. Conclusion This study platform provides a unique longitudinal dataset that allows for the determination and quantification of linkages between human and animal health, including the impact of healthy animals on human disease averted, malnutrition, household educational attainment, and income levels. C1 [Thumbi, S. M.; Marsh, Thomas L.; Noh, Susan; Yoder, Jonathan; Palmer, Guy H.; McElwain, Terry F.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Thumbi, S. M.; Otiang, Elkanah; Ochieng, Linus; Ogola, Eric; Audi, Allan; Bigogo, Godfrey] Kenya Govt Med Res Ctr, Ctr Global Hlth Res, Kisumu, Kenya. [Njenga, M. Kariuki; Munyua, Peninah; Montgomery, Joel M.] Ctr Dis Control & Prevent, Nairobi, Kenya. [Marsh, Thomas L.; Yoder, Jonathan] Washington State Univ, Sch Econ Sci, Pullman, WA 99164 USA. [Noh, Susan] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Breiman, Robert F.] Emory Univ, Atlanta, GA 30322 USA. RP Thumbi, SM (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM thumbi.mwangi@wsu.edu FU Paul G. Allen School for Global Animal Health FX The study was funded through the Paul G. Allen School for Global Animal Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 37 TC 10 Z9 10 U1 3 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 23 PY 2015 VL 10 IS 3 AR UNSP e0120761 DI 10.1371/journal.pone.0120761 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE6ZS UT WOS:000351987300174 PM 25798951 ER PT J AU Zhou, L Wang, SB Jian, JB Geng, QC Wen, J Song, QJ Wu, ZZ Li, GJ Liu, YQ Dunwell, JM Zhang, J Feng, JY Niu, Y Zhang, L Ren, WL Zhang, YM AF Zhou, Ling Wang, Shi-Bo Jian, Jianbo Geng, Qing-Chun Wen, Jia Song, Qijian Wu, Zhenzhen Li, Guang-Jun Liu, Yu-Qin Dunwell, Jim M. Zhang, Jin Feng, Jian-Ying Niu, Yuan Zhang, Li Ren, Wen-Long Zhang, Yuan-Ming TI Identification of domestication-related loci associated with flowering time and seed size in soybean with the RAD-seq genotyping method SO SCIENTIFIC REPORTS LA English DT Article ID MAP-BASED CLONING; GENETIC DIVERSITY; GLYCINE-MAX; POPULATION-STRUCTURE; CROP DOMESTICATION; ARABIDOPSIS; GENOME; WILD; MUTATION; MARKERS AB Flowering time and seed size are traits related to domestication. However, identification of domestication-related loci/genes of controlling the traits in soybean is rarely reported. In this study, we identified a total of 48 domestication-related loci based on RAD-seq genotyping of a natural population comprising 286 accessions. Among these, four on chromosome 12 and additional two on chromosomes 11 and 15 were associated with flowering time, and four on chromosomes 11 and 16 were associated with seed size. Of the five genes associated with flowering time and the three genes associated with seed size, three genes Glyma11g18720, Glyma11g15480 and Glyma15g35080 were homologous to Arabidopsis genes, additional five genes were found for the first time to be associated with these two traits. Glyma11g18720 and Glyma05g28130 were co-expressed with five genes homologous to flowering time genes in Arabidopsis, and Glyma11g15480 was co-expressed with 24 genes homologous to seed development genes in Arabidopsis. This study indicates that integration of population divergence analysis, genome-wide association study and expression analysis is an efficient approach to identify candidate domestication-related genes. C1 [Zhou, Ling; Wang, Shi-Bo; Geng, Qing-Chun; Wen, Jia; Zhang, Jin; Feng, Jian-Ying; Niu, Yuan; Zhang, Li; Ren, Wen-Long; Zhang, Yuan-Ming] Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Natl Ctr Soybean Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China. [Jian, Jianbo; Wu, Zhenzhen] BGI Shenzhen, Shenzhen 518083, Peoples R China. [Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Li, Guang-Jun] Linyi Univ, Coll Life Sci, Linyi 276005, Peoples R China. [Liu, Yu-Qin] Linyi Acad Agr Sci, Crop Res Inst, Linyi 276012, Peoples R China. [Dunwell, Jim M.] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Zhang, Yuan-Ming] Huazhong Agr Univ, Coll Plant Sci & Technol, Stat Genom Lab, Wuhan 430070, Peoples R China. RP Zhang, YM (reprint author), Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Natl Ctr Soybean Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China. EM soyzhang@njau.edu.cn OI Zhang, Yuan-Ming/0000-0003-2317-2190; Dunwell, Jim/0000-0003-2147-665X FU National Key Basic Research Program of China [2011CB109300]; Fundamental Research Funds for the Central Universities [KJQN201414, KJQN201422, KYT201002, KYZ201202-9]; National Natural Science Foundation of China [31301229, 31301004]; Specialised Research Fund for the Doctoral Program of Higher Education [20120097110023]; Priority Academic Program Development of Jiangsu Higher Education Institutions; Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [2014RC020] FX This work was supported by the National Key Basic Research Program of China (grant 2011CB109300), Fundamental Research Funds for the Central Universities (grants KJQN201414, KJQN201422, KYT201002 and KYZ201202-9), the National Natural Science Foundation of China (grants 31301229 and 31301004), a Specialised Research Fund for the Doctoral Program of Higher Education (grant 20120097110023), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (Program No. 2014RC020). The authors declare that they have no conflict of interest. NR 66 TC 8 Z9 8 U1 9 U2 67 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 23 PY 2015 VL 5 AR 9350 DI 10.1038/srep09350 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD8TL UT WOS:000351368800002 PM 25797785 ER PT J AU Henningson, JN Rajao, DS Kitikoon, P Lorusso, A Culhane, MR Lewis, NS Anderson, TK Vincent, AL AF Henningson, Jamie N. Rajao, Daniela S. Kitikoon, Pravina Lorusso, Alessio Culhane, Marie R. Lewis, Nicola S. Anderson, Tavis K. Vincent, Amy L. TI Comparative virulence of wild-type H1N1pdm09 influenza A isolates in swine SO VETERINARY MICROBIOLOGY LA English DT Article DE Pandemic; Influenza A virus; Swine; Pathology; HI; Cross-reactivity ID RESPIRATORY-SYNDROME-VIRUS; H1N1 2009 VIRUS; ORAL FLUID SAMPLES; GENETIC EVOLUTION; HEMAGGLUTININ; INFECTION; PIGS; TRANSMISSION; REPLICATION; SPECIFICITY AB In 2009, a novel swine-origin H1N1 (H1N1pdm09) influenza A virus (IAV) reached pandemic status and was soon after detected in pigs worldwide. The objective of this study was to evaluate whether differences in the HA protein can affect pathogenicity and antigenicity of H1N1pdm09 in swine. We compared lung pathology, viral replication and shedding and the antigenic relationships of four wild-type H1N1pdm09 viruses in pigs: one human (CA/09) and three isolated in swine after the pandemic (IL/09, IL/10, and MN/10). The swine strains were selected based upon unique amino acid substitutions in the HA protein. All selected viruses resulted in mild disease and viral shedding through nasal and oral fluids, however, viral replication and the degree of pathology varied between the isolates. A/Swine/IL/5265/2010 (IL/10), with substitutions I120M, S146G, S186P, V252M, had lower viral titers in the lungs and nasal secretions and fewer lung lesions. The other two swine viruses caused respiratory pathology and replicated to titers similar to the human CA/09, although MN/10 (with mutations D45Y, K304E, A425S) had lower nasal shedding. Swine-adapted H1N1pdm09 have zoonotic potential, and have reassorted with other co-circulating swine viruses, influencing the evolution of IAV in swine globally. Further, our results suggest that amino acid changes in the HA gene have the potential to alter the virulence of H1N1pdm09 in swine. Importantly, the limited clinical signs in pigs could result in continued circulation of these viruses with other endemic swine IAVs providing opportunities for reassortment. Published by Elsevier B.V. C1 [Henningson, Jamie N.; Rajao, Daniela S.; Kitikoon, Pravina; Anderson, Tavis K.; Vincent, Amy L.] USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Henningson, Jamie N.] Kansas State Univ, Dept Diagnost Med Pathobiol, Coll Vet Med, Manhattan, KS 66506 USA. [Lorusso, Alessio] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, Teramo, Italy. [Culhane, Marie R.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. [Lewis, Nicola S.] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England. [Anderson, Tavis K.] Georgia So Univ, Dept Biol, Statesboro, GA 30460 USA. RP Vincent, AL (reprint author), USDA ARS, Virus & Prion Res Unit, NADC, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov RI Lorusso, Alessio/A-7311-2016; OI Lorusso, Alessio/0000-0001-7933-7367; de Souza Rajao, Daniela/0000-0002-0772-0065; Lorusso, Alessio/0000-0001-6156-8212; Anderson, Tavis/0000-0002-3138-5535 FU CNPq-Brazil scholarship recipient; USDA-ARS; DHHS-CDC FX The authors thank Michelle Harland, Gwen Nordholm, Jason Huegel and Jason Crabtree for assistance with laboratory techniques and animal studies. D.S. Rajao was a CNPq-Brazil scholarship recipient. Funding was provided from USDA-ARS and an interagency agreement with DHHS-CDC. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 34 TC 2 Z9 2 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD MAR 23 PY 2015 VL 176 IS 1-2 BP 40 EP 49 DI 10.1016/j.vetmic.2014.12.021 PG 10 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CC1GK UT WOS:000350089000005 PM 25601799 ER PT J AU Shurson, GC Kerr, BJ Hanson, AR AF Shurson, Gerald C. Kerr, Brian J. Hanson, Andrea R. TI Evaluating the quality of feed fats and oils and their effects on pig growth performance SO JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY LA English DT Review DE Growth; Indicative tests; Lipids; Metabolic oxidation; Peroxidation; Pigs ID OXIDIZED VEGETABLE-OILS; ANIMAL FATS; YOUNG-PIGS; NUTRIENT DIGESTIBILITY; BROILER PERFORMANCE; OXIDATIVE STRESS; MEAT QUALITY; FRYING OIL; VITAMIN-E; LIVER AB Feed fats and oils provide significant amounts of energy to swine diets, but there is large variation in composition, quality, feeding value, and price among sources. Common measures of lipid quality include moisture, insolubles, and unsaponifiables (MIU), titer, and free fatty acid content, but provide limited information regarding their feeding value. Lipid peroxidation is an important quality factor related to animal growth performance and health, but maximum tolerable limits in various lipids have not been established. Several indicative assays can be used to detect the presence of various peroxidation compounds, but due to the complexity and numerous compounds produced and degraded during peroxidation process, no single method can adequately determine the extent of peroxidation. Until further information is available, using a combination of peroxide value, thiobarbituric acid reactive substances (TBARS), and anisidine value appear to provide a reasonable assessment of the extent of peroxidation in a lipid at a reasonable cost. However, fatty acid composition of the lipid being evaluated should be considered when selecting specific assays. Predictive tests can also be used to estimate the stability or susceptibility of lipids to peroxidation and include active oxygen method, oil stability index, and oxygen bomb method. A review of 16 published studies with pigs has shown an average decrease of 11.4% in growth rate, 8.8% feed intake fed isocaloric diets containing peroxidized lipids compared to diets containing unperoxidized lipids of the same source. Furthermore, serum vitamin E content was generally reduced and serum TBARS content was increased when peroxidized lipids were fed in these studies, suggesting that feeding peroxidized lipids negatively affects metabolic oxidative status of pigs. However, it is unclear if antioxidants are useful additions to lipids to maintain optimal nutritional value, or if their addition to swine diets is beneficial in overcoming a metabolic oxidative challenge. C1 [Shurson, Gerald C.] Univ Minnesota, Dept Anim Sci, St Paul, MN 55018 USA. [Kerr, Brian J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Hanson, Andrea R.] SVC Res, St Peter, MN 56082 USA. RP Shurson, GC (reprint author), Univ Minnesota, Dept Anim Sci, St Paul, MN 55018 USA. EM shurs001@umn.edu NR 56 TC 8 Z9 8 U1 8 U2 31 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2049-1891 J9 J ANIM SCI BIOTECHNO JI J. Anim. Sci. Biotechnol. PD MAR 21 PY 2015 VL 6 AR 10 DI 10.1186/s40104-015-0005-4 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CE7YF UT WOS:000352057100001 PM 25844168 ER PT J AU Jiao, C Gao, M Wang, XP Fei, ZJ AF Jiao, Chen Gao, Min Wang, Xiping Fei, Zhangjun TI Transcriptome characterization of three wild Chinese Vitis uncovers a large number of distinct disease related genes SO BMC GENOMICS LA English DT Article DE Grape; Chinese wild Vitis; De novo transcriptome; Disease related genes; Cis-NATs ID DOWNY MILDEW RESISTANCE; GRAPEVINE POWDERY MILDEW; ANTISENSE TRANSCRIPTS; DEFENSE RESPONSES; ARABIDOPSIS-THALIANA; EXPRESSION ANALYSIS; UNCINULA-NECATOR; REFERENCE GENOME; PLANT IMMUNITY; LOCUS AB Background: Grape is one of the most valuable fruit crops and can serve for both fresh consumption and wine production. Grape cultivars have been selected and evolved to produce high-quality fruits during their domestication over thousands of years. However, current widely planted grape cultivars suffer extensive loss to many diseases while most wild species show resistance to various pathogens. Therefore, a comprehensive evaluation of wild grapes would contribute to the improvement of disease resistance in grape breeding programs. Results: We performed deep transcriptome sequencing of three Chinese wild grapes using the Illumina strand-specific RNA-Seq technology. High quality transcriptomes were assembled de novo and more than 93% transcripts were shared with the reference PN40024 genome. Over 1,600 distinct transcripts, which were absent or highly divergent from sequences in the reference PN40024 genome, were identified in each of the three wild grapes, among which more than 1,000 were potential protein-coding genes. Gene Ontology (GO) and pathway annotations of these distinct genes showed those involved in defense responses and plant secondary metabolisms were highly enriched. More than 87,000 single nucleotide polymorphisms (SNPs) and 2,000 small insertions or deletions (indels) were identified between each genotype and PN40024, and approximately 20% of the SNPs caused nonsynonymous mutations. Finally, we discovered 100 to 200 highly confident cis-natural antisense transcript (cis-NAT) pairs in each genotype. These transcripts were significantly enriched with genes involved in secondary metabolisms and plant responses to abiotic stresses. Conclusion: The three de novo assembled transcriptomes provide a comprehensive sequence resource for molecular genetic research in grape. The newly discovered genes from wild Vitis, as well as SNPs and small indels we identified, may facilitate future studies on the molecular mechanisms related to valuable traits possessed by these wild Vitis and contribute to the grape breeding programs. Furthermore, we identified hundreds of cis-NAT pairs which showed their potential regulatory roles in secondary metabolism and abiotic stress responses. C1 [Jiao, Chen; Gao, Min; Wang, Xiping] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Jiao, Chen; Gao, Min; Wang, Xiping] Northwest A&F Univ, Minist Agr, Key Lab Hort Plant Biol & Germplasm Innovat North, Yangling 712100, Shaanxi, Peoples R China. [Jiao, Chen; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Wang, XP (reprint author), Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. EM wangxiping@nwsuaf.edu.cn; zf25@cornell.edu OI Fei, Zhangjun/0000-0001-9684-1450 FU National Science Foundation [IOS-0923312, IOS-1313887]; National Natural Science Foundation of China [31272136]; Program for Innovative Research Team of Grape Germplasm Resources and Breeding [2013KCT-25]; China Scholarship Council FX The authors would like to thank Monica Franciscus for proofreading. This work was supported by grants from National Science Foundation (IOS-0923312 and IOS-1313887) to ZF, National Natural Science Foundation of China (grant no. 31272136) and the Program for Innovative Research Team of Grape Germplasm Resources and Breeding (grant no. 2013KCT-25) to XW. CJ was partially supported by a fellowship from China Scholarship Council. NR 86 TC 3 Z9 3 U1 5 U2 43 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 21 PY 2015 VL 16 AR 223 DI 10.1186/s12864-015-1442-3 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CE3LJ UT WOS:000351727500002 PM 25888081 ER PT J AU Zhang, JP Song, QJ Cregan, PB Nelson, RL Wang, XZ Wu, JX Jiang, GL AF Zhang, Jiaoping Song, Qijian Cregan, Perry B. Nelson, Randall L. Wang, Xianzhi Wu, Jixiang Jiang, Guo-Liang TI Genome-wide association study for flowering time, maturity dates and plant height in early maturing soybean (Glycine max) germplasm SO BMC GENOMICS LA English DT Article DE Genetic architecture; Genetic improvement; GWAS; Quantitative trait locus; Single nucleotide polymorphism; Soybean (Glycine max) ID AGRONOMIC TRAITS; GROWTH HABIT; STEM GROWTH; QUANTITATIVE RESISTANCE; ARABIDOPSIS-THALIANA; GENE; CLONING; PHOTOPERIOD; EXPRESSION; MAIZE AB Background: Soybean (Glycine max) is a photoperiod-sensitive and self-pollinated species. Days to flowering (DTF) and maturity (DTM), duration of flowering-to-maturity (DFTM) and plant height (PH) are crucial for soybean adaptability and yield. To dissect the genetic architecture of these agronomically important traits, a population consisting of 309 early maturity soybean germplasm accessions was genotyped with the Illumina Infinium SoySNP50K BeadChip and phenotyped in multiple environments. A genome-wide association study (GWAS) was conducted using a mixed linear model that involves both relative kinship and population structure. Results: The linkage disequilibrium (LD) decayed slowly in soybean, and a substantial difference in LD pattern was observed between euchromatic and heterochromatic regions. A total of 27, 6, 18 and 27 loci for DTF, DTM, DFTM and PH were detected via GWAS, respectively. The Dt1 gene was identified in the locus strongly associated with both DTM and PH. Ten candidate genes homologous to Arabidopsis flowering genes were identified near the peak single nucleotide polymorphisms (SNPs) associated with DTF. Four of them encode MADS-domain containing proteins. Additionally, a pectin lyase-like gene was also identified in a major-effect locus for PH where LD decayed rapidly. Conclusions: This study identified multiple new loci and refined chromosomal regions of known loci associated with DTF, DTM, DFTM and/or PH in soybean. It demonstrates that GWAS is powerful in dissecting complex traits and identifying candidate genes although LD decayed slowly in soybean. The loci and trait-associated SNPs identified in this study can be used for soybean genetic improvement, especially the major-effect loci associated with PH could be used to improve soybean yield potential. The candidate genes may serve as promising targets for studies of molecular mechanisms underlying the related traits in soybean. C1 [Zhang, Jiaoping; Wang, Xianzhi; Wu, Jixiang; Jiang, Guo-Liang] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57006 USA. [Song, Qijian; Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Nelson, Randall L.] Univ Illinois, USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. [Nelson, Randall L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Jiang, Guo-Liang] Virginia State Univ, Agr Res Stn, Petersburg, VA 23806 USA. RP Jiang, GL (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57006 USA. EM gjiang@vsu.edu FU South Dakota Agricultural Experiment Station; USDA-NIFA/CSREES Hatch project; United Soybean Board; South Dakota Soybean Research and Promotion Council FX The authors are grateful to Siddhi Bhusal, Nicholas Hall, Marci Green, Sivananda Tirumalaraju and Mukhtar Agoub for assistance in implementing the field experiments, and Alexander E. Lipka, Institute for Genomic Diversity, Cornell University, for assistance in using GAPIT R package. This research was supported in part by the South Dakota Agricultural Experiment Station and USDA-NIFA/CSREES Hatch project, and the grants of the United Soybean Board and the South Dakota Soybean Research and Promotion Council awarded to G.-L. Jiang. Virginia State University Agricultural Research Station provided support in completion of the manuscript. NR 52 TC 17 Z9 18 U1 6 U2 37 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 20 PY 2015 VL 16 AR 217 DI 10.1186/s12864-015-1441-4 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CL4HN UT WOS:000356913300001 PM 25887991 ER PT J AU Ammar, ED Hentz, M Hall, DG Shatters, RG AF Ammar, El-Desouky Hentz, Matthew Hall, David G. Shatters, Robert G., Jr. TI Ultrastructure of Wax-Producing Structures on the Integument of the Melaleuca Psyllid Boreioglycaspis melaleucae (Hemiptera: Psyllidae), with Honeydew Excretion Behavior in Males and Females SO PLOS ONE LA English DT Article ID BIOLOGICAL-CONTROL AGENT; DIAPHORINA-CITRI; FINE-STRUCTURE; SCALE INSECTS; HOST-RANGE; QUINQUENERVIA; HOMOPTERA; GLANDS; APHID; PSYLLOIDEA AB The melaleuca psyllid, Boreioglycaspis melaleucae (Hemiptera: Psyllidae), was introduced to Florida as a biological control agent against Melaleuca quinquenervia, an invasive evergreen tree that has invaded large areas of Florida Everglades. Colonies of B. melaleucae nymphs are normally covered by white waxy secretions, and nymphs of various instars produce long bundles of white waxy filaments extending laterally and posteriorly from their abdomen. Scanning electron microscopy of 'naturally waxed' and 'dewaxed' nymphs (cleaned from wax) revealed two types of wax pore plates located dorsally and laterally on the integument of posterior abdominal segments starting with the 4th segment. Type-1 wax pore plates, with raised rim, peripheral groove, slits and pits, produce long ribbons and filaments of waxy secretions that are wound together forming long wax bundles, whereas type-2 wax pore plates, with slits only, produce shorter wax curls. Additionally, in both nymphs and adult females, the circumanal ring contained ornate rows of wax pores that produce wax filaments covering their honeydew excretions. Video recordings with stereomicroscopy showed that adult females produce whitish honeydew balls, powerfully propelled away from their body, probably to get these sticky excretions away from their eggs and newly hatched nymphs. Adult males, however, produce clear droplets of honeydew immediately behind them, simply by bending the posterior end of the abdomen downward. The possible role(s) of waxy secretions by nymphs and adults of B. melaleucae in reducing contamination of their colonies with honeydew, among other possibilities, are discussed. C1 [Ammar, El-Desouky; Hentz, Matthew; Hall, David G.; Shatters, Robert G., Jr.] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. RP Ammar, ED (reprint author), USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. EM desoukyammar@gmail.com FU Agricultural Research Service, United States Department of Agriculture FX Funding: This work was supported by Agricultural Research Service, United States Department of Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 28 TC 0 Z9 0 U1 5 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 20 PY 2015 VL 10 IS 3 AR e0121354 DI 10.1371/journal.pone.0121354 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE8HY UT WOS:000352083900126 PM 25793934 ER PT J AU Castaneda-Ortiz, EJ Ueti, MW Camacho-Nuez, M Mosqueda, JJ Mousel, MR Johnson, WC Palmer, GH AF Castaneda-Ortiz, Elizabeth J. Ueti, Massaro W. Camacho-Nuez, Minerva Mosqueda, Juan J. Mousel, Michelle R. Johnson, Wendell C. Palmer, Guy H. TI Association of Anaplasma marginale Strain Superinfection with Infection Prevalence within Tropical Regions SO PLOS ONE LA English DT Article ID OUTER-MEMBRANE PROTEINS; BOOPHILUS-MICROPLUS; ANTIGENIC VARIATION; TRANSMISSION; CONSERVATION; DIVERSITY; TICK; EXPRESSION; PATHOGENS; IXODIDAE AB Strain superinfection occurs when a second strain infects a host already infected with and having mounted an immune response to a primary strain. The incidence of superinfection with Anaplasma marginale, a tick-borne rickettsial pathogen of domestic and wild ruminants, has been shown to be higher in tropical versus temperate regions. This has been attributed to the higher prevalence of infection, with consequent immunity against primary strains and thus greater selective pressure for superinfection with antigenically distinct strains. However an alternative explanation would be the differences in the transmitting vector, Dermacentor andersoni in the studied temperate regions and Rhipicephalus microplus in the studied tropical regions. To address this question, we examined two tropical populations sharing the same vector, R. microplus, but with significantly different infection prevalence. Using two separate markers, msp1 alpha (one allele per genome) and msp2 (multiple alleles per genome), there were higher levels of multiple strain infections in the high infection prevalence as compared to the low prevalence population. The association of higher strain diversity with infection prevalence supports the hypothesis that high levels of infection prevalence and consequent population immunity is the predominant driver of strain superinfection. C1 [Castaneda-Ortiz, Elizabeth J.; Camacho-Nuez, Minerva] Univ Autonoma Ciudad Mexico, Posgrad Ciencias Genom, Mexico City, DF, Mexico. [Ueti, Massaro W.; Mousel, Michelle R.; Johnson, Wendell C.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [Ueti, Massaro W.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Mosqueda, Juan J.] Univ Autonoma Queretaro, Juriquilla, Mexico. [Palmer, Guy H.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. RP Palmer, GH (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM gpalmer@vetmed.wsu.edu OI Mousel, Michelle/0000-0003-1367-7005 FU Universidad Autonoma de la Ciudad de Mexico; U.S. Department of Agriculture, Agricultural Research Service [5348-32000-033-00D]; National Institutes of Health [R37 AI44005] FX Elizabeth J. Castaneda Ortiz was supported by Universidad Autonoma de la Ciudad de Mexico. This research was supported by the U.S. Department of Agriculture, Agricultural Research Service Grant 5348-32000-033-00D (MWU, MRM, WCJ) and the National Institutes of Health R37 AI44005 (GHP). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 26 TC 7 Z9 7 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 20 PY 2015 VL 10 IS 3 AR e0120748 DI 10.1371/journal.pone.0120748 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE8HY UT WOS:000352083900072 PM 25793966 ER PT J AU Kritchevsky, SB Beavers, KM Miller, ME Shea, MK Houston, DK Kitzman, DW Nicklas, BJ AF Kritchevsky, Stephen B. Beavers, Kristen M. Miller, Michael E. Shea, M. Kyla Houston, Denise K. Kitzman, Dalane W. Nicklas, Barbara J. TI Intentional Weight Loss and All-Cause Mortality: A Meta-Analysis of Randomized Clinical Trials SO PLOS ONE LA English DT Article ID OBESE OLDER-ADULTS; LIFE-STYLE INTERVENTION; HIGH BLOOD-PRESSURE; BODY-MASS INDEX; PHYSICAL-ACTIVITY; NONPHARMACOLOGIC INTERVENTIONS; HYPERTENSION PREVENTION; KNEE OSTEOARTHRITIS; DIABETES-MELLITUS; SODIUM REDUCTION AB Background Obesity is associated with increased mortality, and weight loss trials show rapid improvement in many mortality risk factors. Yet, observational studies typically associate weight loss with higher mortality risk. The purpose of this meta-analysis of randomized controlled trials (RCTs) of weight loss was to clarify the effects of intentional weight loss on mortality. Methods 2,484 abstracts were identified and reviewed in PUBMED, yielding15 RCTs reporting (1) randomization to weight loss or non-weight loss arms, (2) duration of >= 18 months, and (3) deaths by intervention arm. Weight loss interventions were all lifestyle-based. Relative risks (RR) and 95% confidence intervals (95% CI) were estimated for each trial. For trials reporting at least one death (n = 12), a summary estimate was calculated using the Mantel-Haenszel method. Sensitivity analysis using sparse data methods included remaining trials. Results Trials enrolled 17,186 participants (53% female, mean age at randomization = 52 years). Mean body mass indices ranged from 30-46 kg/m(2), follow-up times ranged from 18 months to 12.6 years (mean: 27 months), and average weight loss in reported trials was 5.5 +/- 4.0 kg. A total of 264 deaths were reported in weight loss groups and 310 in non-weight loss groups. The weight loss groups experienced a 15% lower all-cause mortality risk (RR = 0.85; 95% CI: 0.73-1.00). There was no evidence for heterogeneity of effect (Cochran's Q = 5.59 (11 d.f.; p = 0.90); I-2 = 0). Results were similar in trials with a mean age at randomization >= 55 years (RR = 0.84; 95% CI 0.71-0.99) and a follow-up time of >= 4 years (RR = 0.85; 95% CI 0.72-1.00). Conclusions In obese adults, intentional weight loss may be associated with approximately a 15% reduction in all-cause mortality. C1 [Kritchevsky, Stephen B.; Beavers, Kristen M.; Miller, Michael E.; Houston, Denise K.; Kitzman, Dalane W.; Nicklas, Barbara J.] Wake Forest Univ, Bowman Gray Sch Med, Dept Internal Med, Winston Salem, NC 27103 USA. [Beavers, Kristen M.] Wake Forest Univ, Dept Hlth & Exercise Sci, Winston Salem, NC 27103 USA. [Miller, Michael E.] Wake Forest Sch Med, Dept Biostat Sci, Winston Salem, NC USA. [Shea, M. Kyla] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Kritchevsky, SB (reprint author), Wake Forest Univ, Bowman Gray Sch Med, Dept Internal Med, Winston Salem, NC 27103 USA. EM skritche@wakehealth.edu FU Wake Forest Older Americans Independence Center [P30 AG021332]; [R01 AG033087] FX This work was supported by the Wake Forest Older Americans Independence Center (P30 AG021332) and by R01 AG033087. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 46 TC 19 Z9 21 U1 3 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 20 PY 2015 VL 10 IS 3 AR e0121993 DI 10.1371/journal.pone.0121993 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE8HY UT WOS:000352083900163 PM 25794148 ER PT J AU Salem, M Paneru, B Al-Tobasei, R Abdouni, F Thorgaard, GH Rexroad, CE Yao, JB AF Salem, Mohamed Paneru, Bam Al-Tobasei, Rafet Abdouni, Fatima Thorgaard, Gary H. Rexroad, Caird E. Yao, Jianbo TI Transcriptome Assembly, Gene Annotation and Tissue Gene Expression Atlas of the Rainbow Trout SO PLOS ONE LA English DT Article ID 3 ABUNDANCE CLASSES; RNA-SEQ DATA; ONCORHYNCHUS-MYKISS; MESSENGER-RNA; GENOME DUPLICATION; ATLANTIC SALMON; INTEGRATED MAP; FISH GENOMES; SKIN MUCUS; GENERATION AB Efforts to obtain a comprehensive genome sequence for rainbow trout are ongoing and will be complemented by transcriptome information that will enhance genome assembly and annotation. Previously, transcriptome reference sequences were reported using data from different sources. Although the previous work added a great wealth of sequences, a complete and well-annotated transcriptome is still needed. In addition, gene expression in different tissues was not completely addressed in the previous studies. In this study, non-normalized cDNA libraries were sequenced from 13 different tissues of a single doubled haploid rainbow trout from the same source used for the rainbow trout genome sequence. A total of similar to 1.167 billion paired-end reads were de novo assembled using the Trinity RNA-Seq assembler yielding 474,524 contigs > 500 base-pairs. Of them, 287,593 had homologies to the NCBI non-redundant protein database. The longest contig of each cluster was selected as a reference, yielding 44,990 representative contigs. A total of 4,146 contigs (9.2%), including 710 full-length sequences, did not match any mRNA sequences in the current rainbow trout genome reference. Mapping reads to the reference genome identified an additional 11,843 transcripts not annotated in the genome. A digital gene expression atlas revealed 7,678 housekeeping and 4,021 tissue-specific genes. Expression of about 16,000-32,000 genes (35-71% of the identified genes) accounted for basic and specialized functions of each tissue. White muscle and stomach had the least complex transcriptomes, with high percentages of their total mRNA contributed by a small number of genes. Brain, testis and intestine, in contrast, had complex transcriptomes, with a large numbers of genes involved in their expression patterns. This study provides comprehensive de novo transcriptome information that is suitable for functional and comparative genomics studies in rainbow trout, including annotation of the genome. C1 [Salem, Mohamed; Paneru, Bam; Al-Tobasei, Rafet; Abdouni, Fatima] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA. [Thorgaard, Gary H.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Thorgaard, Gary H.] Washington State Univ, Ctr Reprod Biol, Pullman, WA 99164 USA. [Rexroad, Caird E.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Leetown, WV 25430 USA. [Yao, Jianbo] W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. RP Salem, M (reprint author), Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA. EM Mohamed.salem@mtsu.edu FU United States Department of Agriculture, Agriculture and Food Research [58-1930-0-059]; United States Department of Agriculture, National Institute of Food and Agriculture [2014-67015-21602] FX Funding: This study was supported by a cooperative agreement grant No. 58-1930-0-059 from the United States Department of Agriculture, Agriculture and Food Research (JY); and a competitive grant No. 2014-67015-21602 from the United States Department of Agriculture, National Institute of Food and Agriculture (MS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 94 TC 6 Z9 6 U1 3 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 20 PY 2015 VL 10 IS 3 AR e0121778 DI 10.1371/journal.pone.0121778 PG 27 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CE8HY UT WOS:000352083900152 PM 25793877 ER PT J AU Wu, ZL Banuelos, GS Lin, ZQ Liu, Y Yuan, LX Yin, XB Li, M AF Wu, Zhilin Banuelos, Gary S. Lin, Zhi-Qing Liu, Ying Yuan, Linxi Yin, Xuebin Li, Miao TI Biofortification and phytoremediation of selenium in China SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE biofortification; phytoremediation; selenium; deficiency; nutrient ID HYPERACCUMULATOR STANLEYA-PINNATA; TRITICUM-AESTIVUM L.; FOOD CROPS; SELENOCYSTEINE METHYLTRANSFERASE; ASTRAGALUS RACEMOSUS; SE BIOFORTIFICATION; FOLIAR APPLICATION; ENHANCED SELENIUM; RHIZOSPHERE FUNGI; CANCER PREVENTION AB Selenium (Se) is an essential trace element for humans and animals but at high concentrations, Se becomes toxic to organisms due to Se replacing sulfur in proteins. Selenium biofortification is an agricultural process that increases the accumulation of Se in crops, through plant breeding, genetic engineering, or use of Se fertilizers. Selenium phytoremediation is a green biotechnology to clean up Se-contaminated environments, primarily through phytoextraction and phytovolatilization. By integrating Se phytoremediation and biofortification technologies, Se-enriched plant materials harvested from Se phytoremediation can be used as Se-enriched green manures or other supplementary sources of Se for producing Se-biofortified agricultural products. Earlier studies primarily aimed at enhancing efficacy of phytoremediation and biofortification of Se based on natural variation in progenitor or identification of unique plant species. In this review, we discuss promising approaches to improve biofortification and phytoremediation of Se using knowledge acquired from model crops. We also explored the feasibility of applying biotechnologies such as inoculation of microbial strains for improving the efficiency of biofortification and phytoremediation of Se. The key research and practical challenges that remain in improving biofortification and phytoremediation of Se have been highlighted, and the future development and uses of Se-biofortified agricultural products in China has also been discussed. C1 [Wu, Zhilin; Li, Miao] Anhui Agr Univ, Sch Resources & Environm, Sch Plant Protect, Key Lab Agri Food Safety Anhui Prov, Hefei, Peoples R China. [Wu, Zhilin; Liu, Ying; Yuan, Linxi; Yin, Xuebin] Univ Sci & Technol China, Suzhou Inst Adv Study, Bioengn Res Ctr Selenium, Adv Lab Selenium & Human Hlth Jiangsu, Suzhou 215123, Jiangsu, Peoples R China. [Wu, Zhilin; Liu, Ying; Yuan, Linxi; Yin, Xuebin] Univ Sci & Technol China, Sch Earth & Space Sci, Hefei 230026, Peoples R China. [Banuelos, Gary S.] USDA ARS, Parlier, CA USA. [Lin, Zhi-Qing] So Illinois Univ, Dept Biol Sci, Edwardsville, IL 62026 USA. [Lin, Zhi-Qing] So Illinois Univ, Environm Sci Program, Edwardsville, IL 62026 USA. RP Li, M (reprint author), Anhui Agr Univ, Sch Resources & Environm, Sch Plant Protect, Key Lab Agri Food Safety Anhui Prov, Hefei, Peoples R China. EM yuanli@ustc.edu.cn; xbyin@ustc.edu.cn; miaoli@ustc.edu.cn FU National Natural Science Foundation of China [31400091, 50949038]; Natural Science Foundation of Anhui province [1408085MC68]; Natural Science Foundation of Jiangsu Province [BK2012195, BK2012202]; Jiangsu Provincial Postdoctoral Science Foundation [1302080C]; China Postdoctoral Science Foundation [20100470108, 2013M540518] FX We thank all members of Advanced Laboratory for Selenium and Human Health, University of Science and Technology of China for their assistance during the preparation of this manuscript. This work was supported by the National Natural Science Foundation of China (Grant 31400091 and 50949038), the Natural Science Foundation of Anhui province (Grant 1408085MC68), the Natural Science Foundation of Jiangsu Province (Grant BK2012195 and BK2012202), Jiangsu Provincial Postdoctoral Science Foundation (Grant 1302080C), and China Postdoctoral Science Foundation (Grant 20100470108 and 2013M540518). NR 112 TC 16 Z9 17 U1 30 U2 102 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 20 PY 2015 VL 6 AR 136 DI 10.3389/fpls.2015.00136 PG 8 WC Plant Sciences SC Plant Sciences GA CE9FV UT WOS:000352150300001 PM 25852703 ER PT J AU Bellaloui, N Stetina, SR Turley, RB AF Bellaloui, Nacer Stetina, Salliana R. Turley, Rickie B. TI Cottonseed protein, oil, and mineral status in near-isogenic Gossypium hirsutum cotton lines expressing fuzzy/linted and fuzzless/linted seed phenotypes under field conditions SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE cottonseed protein; cottonseed oil; cottonseed composition; minerals; near-isogenic cotton; cotton mutants ID SOYBEAN PRODUCTION SYSTEM; FOLIAR BORON APPLICATION; AZOMETHINE-H METHOD; POLLEN-TUBE GROWTH; CELL ELONGATION; NUTRIENT COMPOSITION; FIBER DEVELOPMENT; GENE-EXPRESSION; PLANTS; CALCIUM AB Cotton is an important crop in the world and is a major source of oil for human consumption and cotton meal for livestock. Cottonseed nutrition (seed composition: protein, oil, and minerals) determines the quality of seeds. Therefore, maintaining optimum levels of cottonseed nutrition is critical. Physiological and genetic mechanisms controlling the levels of these constituents in cottonseed are still largely unknown. Our previous research conducted under greenhouse conditions showed that seed and leaf nutrition differed between fuzzless and fuzzy seed isolines. Therefore, the objective of this research was to investigate the seed fuzz phenotype (trait) effects on seed protein, oil, N, C, S, and minerals in five sets of near-isogenic mutant cotton lines for seed fuzz in a 2-year experiment under field condition to evaluate the stability of the effect of the trait on seed nutrition. The isolines (genotypes) in each set differ for the seed fuzz trait (fuzzless/linted seed line, N lines, and fuzzy/linted seed line, F lines). Results showed that seed protein was higher in the fuzzy genotype in all sets, but seed oil was higher in fuzzless genotype in all sets. The concentrations of seed Ca and C were higher in all fuzzless genotypes, but N, S, B, Fe, and Zn were higher in most of the fuzzy genotypes. Generally, minerals were higher in leaves of F lines, suggesting the translocation of minerals from leaves to seeds was limited. The research demonstrated that fiber development could be involved in cottonseed composition. This may be due to the involvement of fiber development in carbon and nitrogen metabolism, and the mobility of nutrients from leaves (source) to seed (sink). This information is beneficial to breeders to consider fuzzless cottonseed for potential protein and oil use and select for higher oil or higher protein content, and to physiologists to further understand the mobility of minerals to increase the quality of cottonseed nutrition for food and feed. C1 [Bellaloui, Nacer; Stetina, Salliana R.; Turley, Rickie B.] ARS, USDA, Crop Genet Res Unit, Plant Physiol, Stoneville, MS 38776 USA. RP Bellaloui, N (reprint author), ARS, USDA, Crop Genet Res Unit, Res Plant Physiol, 141 Expt Stn Rd,POB 345, Stoneville, MS 38776 USA. EM nacer.bellaloui@ars.usda.gov FU USDA, Agricultural Research Service [6066-21220-012-00D, 6066-21000-051-00D, 6066-22000-074-00D] FX Technical support for this project provided by S. Mosley, K Jordan, and R. Manning is appreciated. Funding for this research was provided by USDA, Agricultural Research Service projects 6066-21220-012-00D, 6066-21000-051-00D, and 6066-22000-074-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the Unites States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 71 TC 2 Z9 2 U1 1 U2 12 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 19 PY 2015 VL 6 AR 137 DI 10.3389/105-2015.00137 PG 14 WC Plant Sciences SC Plant Sciences GA CE9FN UT WOS:000352149500001 PM 25852704 ER PT J AU Zhu, YR Wu, FC He, ZQ Giesy, JP Feng, WY Mu, YS Feng, CL Zhao, XL Liao, HQ Tang, Z AF Zhu, Yuanrong Wu, Fengchang He, Zhongqi Giesy, John P. Feng, Weiying Mu, Yunsong Feng, Chenglian Zhao, Xiaoli Liao, Haiqing Tang, Zhi TI Influence of natural organic matter on the bioavailability and preservation of organic phosphorus in lake sediments SO CHEMICAL GEOLOGY LA English DT Article DE Enzymatic hydrolysis; P-31 NMR; Organic phosphorus; Bioavailability; Preservation; Natural organic matter ID MAGNETIC-RESONANCE-SPECTROSCOPY; HUMIC-ACID FRACTIONS; CAPILLARY-ZONE-ELECTROPHORESIS; SHALLOW EUTROPHIC LAKE; FRESH-WATER SEDIMENTS; ALKALINE-PHOSPHATASE; ENZYMATIC-HYDROLYSIS; P-31 NMR; BIOGENIC PHOSPHORUS; ETHYLENEDIAMINETETRAACETIC ACID AB Information about the bioavailability and sequestration of organic phosphorus (Po) in sediments is fundamental to understanding biogeochemical cycling of phosphorus (P) in eutrophic lakes. However, the processes governing preservation of Po in sediments are still poorly understood. Sequential extraction of P-o by H2O (H2O-P-o) and NaOH-EDTA (NaOH-EDTA P-o), in combination with enzymatic hydrolysis/P-31 NMR, was applied to estimate the bioavailability of Po in sediments of Lake Tai (Ch: Taihu), China. Of H2O-P-o and NaOH-EDTA P-o, 45.5-89.4% and 30.4-71.3% respectively were hydrolyzed by phosphatase, and therefore considered to be biologically available. Of NaOH-EDTA Po, 28.7-69.6% could not be hydrolyzed by phosphatase; this portion was characterized by P-31 NMR as monoester P and/or diester P. Simulation experiments of hydrolysis of model P-o compounds in the presence of humic acids (HA), which were used as a model for natural organic matter (NOM), and metals, including Al, Ca, and Fe, have demonstrated that enzymatic hydrolysis of labile monoester P was weakly reduced by HA or metal ions. Condensed phosphate (e.g., pyrophosphate) and phytate-like P (e.g., inositol phosphates) were resistant to enzymatic hydrolysis in the presence of HA and/or metal ions, which indicated that they may be possibly preserved in sediments. These observations suggest that NOM in sediments can be a significant factor determining the bioavailability and preservation of P-o in sediments. The presence of metals would enhance the effect of NOM on preservation of P-o in sediments. Formation of P-o-metal-HA or P-o-metal complexes might be mechanisms responsible for these processes. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhu, Yuanrong; Wu, Fengchang; Feng, Weiying; Mu, Yunsong; Feng, Chenglian; Zhao, Xiaoli; Liao, Haiqing; Tang, Zhi] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. [Zhu, Yuanrong; Feng, Weiying] Beijing Normal Univ, Coll Water Sci, Beijing 100875, Peoples R China. [He, Zhongqi] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Giesy, John P.] Univ Saskatchewan, Dept Biomed Vet Biosci, Saskatoon, SK, Canada. [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK, Canada. [Giesy, John P.] City Univ Hong Kong, Dept Biol & Chem, Kowloon, Hong Kong, Peoples R China. [Giesy, John P.] City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon, Hong Kong, Peoples R China. RP Wu, FC (reprint author), Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. EM wufengchang@vip.skleg.cn FU National Natural Science Foundation of China [41130743, 41403094, 41261140337] FX This research was jointly supported by the National Natural Science Foundation of China (Nos. 41130743, 41403094, 41261140337). NR 71 TC 11 Z9 12 U1 8 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0009-2541 EI 1878-5999 J9 CHEM GEOL JI Chem. Geol. PD MAR 18 PY 2015 VL 397 BP 51 EP 60 DI 10.1016/j.chemgeo.2015.01.006 PG 10 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA CE2TP UT WOS:000351671900005 ER PT J AU Thompson, D Bhatt, R Vazquez, I Cullen, KW Baranowski, J Baranowski, T Liu, Y AF Thompson, Debbe Bhatt, Riddhi Vazquez, Isabel Cullen, Karen W. Baranowski, Janice Baranowski, Tom Liu, Yan TI Creating action plans in a serious video game increases and maintains child fruit-vegetable intake: a randomized controlled trial SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Videogame; Fruit; Vegetables; Children; Implementation intentions; Maintenance ID PHYSICAL-ACTIVITY INTERVENTIONS; ACTIVITY BEHAVIOR-CHANGE; AFRICAN-AMERICAN GIRLS; IMPLEMENTATION INTENTIONS; DIETARY PATTERNS; CONSUMPTION; STUDENTS; PROGRAM; HEALTH; METAANALYSIS AB Background: Child fruit and vegetable intake is below recommended levels, increasing risk for chronic disease. Interventions to influence fruit and vegetable intake among youth have had mixed effects. Innovative, theory-driven interventions are needed. Goal setting, enhanced by implementation intentions (i.e., plans tightly connected to a behavioral goal), may offer a solution. Action plans state "how" a goal will be achieved, while coping plans identify a potential barrier and corresponding solution. The research reported here evaluated the short-and long-term effects of goal setting enhanced with implementation intentions on child fruit and vegetable intake in a 10-episode, theoretically-grounded serious videogame promoting fruit and vegetables. This is one of the first studies to test the efficacy of implementation intentions on the dietary intake of healthy children. Methods: A four-group randomized design with three data collection periods (baseline, immediate post-intervention, 3 months post-intervention) was employed. Groups varied on whether children created an implementation intention (none, action, coping, both) as part of goal setting. Participants were 4th and 5th grade children (similar to 9-11 years old) and one parent. An a priori power analysis indicated this would provide >80% power to detect a small effect (Cohen's d = 0.17). Children played a 10-episode online videogame; parents received 10 electronic newsletters and access to a parent-only website. The primary outcome was child fruit and vegetable intake, assessed via three, dietitian-assisted telephone recalls at each data collection period. The primary analysis was conducted using a repeated measures analysis of covariance with a mixed model procedure. Secondary analyses examined intervention effects on fruit and vegetables separately. Results: Four hundred parent/child dyads were recruited. A significant group-by-time interaction for fruit and vegetable intake (p < 0.001) was found in only the Action group, which had significant increases in fruit and vegetable intake at post 1 (p < 0.0001) and post 2 (p < 0.0001). No other significant interactions were observed; however, there were significant time effects for fruit (p < 0.0001). Conclusions: Action intentions may be an important component of successful interventions to increase and maintain fruit and vegetable intake in pre-adolescent children. Videogames promoting healthy diets offer an effective vehicle for delivering behavior change interventions to children. C1 [Thompson, Debbe; Vazquez, Isabel; Cullen, Karen W.; Baranowski, Janice; Baranowski, Tom; Liu, Yan] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77056 USA. [Bhatt, Riddhi] Baylor Coll Med, Dept Family & Community Med, Houston, TX 77030 USA. RP Thompson, D (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77056 USA. EM dit@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institutes of Health, National Institute of Child Health & Human Development [HD050595]; USDA/ARS [58-6250-0-008] FX This work is a publication of the United States Department of Agriculture/Agricultural Research Center (USDA/ARS), Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas. This project was supported by the National Institutes of Health, National Institute of Child Health & Human Development grant #HD050595 (to Dr. Thompson). This work is also a publication of the USDA/ARS, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. We would like to thank the children and parents who participated in this research, and the SQ2 data collection and intervention staffs. We would also like to extend our thanks to Archimage, Inc. of Houston, TX for its game design services. NR 59 TC 8 Z9 8 U1 3 U2 26 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD MAR 18 PY 2015 VL 12 AR 39 DI 10.1186/s12966-015-0199-z PG 10 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA CE0NG UT WOS:000351500300001 PM 25890060 ER PT J AU Huang, HB Qureshi, N Chen, MH Liu, W Singh, V AF Huang, Haibo Qureshi, Nasib Chen, Ming-Hsu Liu, Wei Singh, Vijay TI Ethanol Production from Food Waste at High Solids Content with Vacuum Recovery Technology SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE food waste; ethanol; fermentation; vacuum recovery; high solids content ID SACCHAROMYCES-CEREVISIAE; ENZYMATIC SACCHARIFICATION; WHEAT-STRAW; CORN STOVER; FERMENTATION; OPTIMIZATION; HYDROLYSIS; TEMPERATURE; SUGARCANE; STARCH AB Ethanol production from food wastes does not only solve environmental issues but also provides renewable biofuels. This study investigated the feasibility of producing ethanol from food wastes at high solids content (35%, w/w). A vacuum recovery system was developed and applied to remove ethanol from fermentation broth to reduce yeast ethanol inhibition. A high concentration of ethanol (144 g/L) was produced by the conventional fermentation of food waste without a vacuum recovery system. When the vacuum recovery is applied to the fermentation process, the ethanol concentration in the fermentation broth was controlled below 100 g/L, thus reducing yeast ethanol inhibition. At the end of the conventional fermentation, the residual glucose in the fermentation broth was 5.7 g/L, indicating incomplete utilization of glucose, while the vacuum fermentation allowed for complete utilization of glucose. The ethanol yield for the vacuum fermentation was found to be 358 g/kg of food waste (dry basis), higher than that for the conventional fermentation at 327 g/kg of food waste (dry basis). C1 [Huang, Haibo; Chen, Ming-Hsu; Liu, Wei; Singh, Vijay] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. [Qureshi, Nasib] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Singh, V (reprint author), Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. EM vsingh@illinois.edu NR 39 TC 8 Z9 8 U1 2 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 18 PY 2015 VL 63 IS 10 BP 2760 EP 2766 DI 10.1021/jf5054029 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CD9LS UT WOS:000351420400022 PM 25706565 ER PT J AU Allen, HK An, R Handelsman, J Moe, LA AF Allen, Heather K. An, Ran Handelsman, Jo Moe, Luke A. TI A Response Regulator from a Soil Metagenome Enhances Resistance to the beta-Lactam Antibiotic Carbenicillin in Escherichia coli SO PLOS ONE LA English DT Article ID 2-COMPONENT SIGNAL-TRANSDUCTION; FUNCTIONAL METAGENOMICS; ALASKAN SOIL; GENES; PROTEINS; PERMEABILITY; EVOLUTION; BACTERIA; MUTANTS; STRESS AB Functional metagenomic analysis of soil metagenomes is a method for uncovering as-yet unidentified mechanisms for antibiotic resistance. Here we report an unconventional mode by which a response regulator derived from a soil metagenome confers resistance to the beta-lactam antibiotic carbenicillin in Escherichia coli. A recombinant clone (beta lr16) harboring a 5,169 bp DNA insert was selected from a metagenomic library previously constructed from a remote Alaskan soil. The beta lr16 clone conferred specific resistance to carbenicillin, with limited increases in resistance to other tested antibiotics, including other beta-lactams (penicillins and cephalosporins), rifampin, ciprofloxacin, erythromycin, chloramphenicol, nalidixic acid, fusidic acid, and gentamicin. Resistance was more pronounced at 24 degrees C than at 37 degrees C. Zone-of-inhibition assays suggested that the mechanism of carbenicillin resistance was not due to antibiotic inactivation. The DNA insert did not encode any genes known to confer antibiotic resistance, but did have two putative open reading frames (ORFs) that were annotated as a metallopeptidase and a two-component response regulator. Transposon mutagenesis and subcloning of the two ORFs followed by phenotypic assays showed that the response regulator gene was necessary and sufficient to confer the resistance phenotype. Quantitative reverse transcriptase PCR showed that the response regulator suppressed expression of the ompF porin gene, independently of the small RNA regulator micF, and enhanced expression of the acrD, mdtA, and mdtB efflux pump genes. This work demonstrates that antibiotic resistance can be achieved by the modulation of gene regulation by heterologous DNA. Functional analyses such as these can be important for making discoveries in antibiotic resistance gene biology and ecology. C1 [Allen, Heather K.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [An, Ran; Moe, Luke A.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Handelsman, Jo] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA. RP Moe, LA (reprint author), Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. EM luke.moe@uky.edu FU United States Department of Agriculture National Institute of Food and Agriculture [2011-67020-30195]; National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service [WIS01312] FX This work was funded in part by grants from United States Department of Agriculture National Institute of Food and Agriculture (2011-67020-30195) and the National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service (WIS01312) to LAM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 3 Z9 3 U1 5 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 17 PY 2015 VL 10 IS 3 AR e0120094 DI 10.1371/journal.pone.0120094 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD7QC UT WOS:000351284600125 PM 25782011 ER PT J AU dos Santos, CR Cordeiro, RL Wong, DWS Murakami, MT AF dos Santos, Camila Ramos Cordeiro, Rosa Lorizolla Wong, Dominic W. S. Murakami, Mario Tyago TI Structural Basis for Xyloglucan Specificity and alpha-D-Xylp(1 -> 6)-D-Glcp Recognition at the-1 Subsite within the GH5 Family SO BIOCHEMISTRY LA English DT Article ID END-SPECIFIC CELLOBIOHYDROLASE; CELL WALL COMPOSITION; CRYSTAL-STRUCTURE; MACROMOLECULAR CRYSTALLOGRAPHY; SUBSTRATE-SPECIFICITY; MOLECULAR REPLACEMENT; CELLULOSE SURFACES; CLONING; ENDO-BETA-1,4-GLUCANASE; ENDOGLUCANASE AB GH5 is one of the largest glycoside hydrolase families, comprising at least 20 distinct activities within a common structural scaffold. However, the molecular basis for the functional differentiation among GH5 Members is still not fully understood, principally for xyloglucan specificity. In this work, we elucidated the crystal structures of two novel GH5 xyloglucanases (XEGs) retrieved from a rumen microflora metagenomic library, in the native state and in complex with xyloglucan-derived oligosaccharides. These results provided insights into the structural determinants that differentiate GH5 XEGs from parental cellulases and a new mode of action within the GH5 family related to structural adaptations in the -1 subsite. The oligosaccharide found in the XEG5A complex, permitted the mapping, for the first time; of the positive subsites of a GH5 XEG, revealing the importance of the pocket-like topology of the +1 subsite in conferring the ability of some GH5 enzymes to attack xyloglucan. Complementarily, the XEG5B complex covered the negative subsites, completing the subsite mapping of GH5 XEGs at high resolution. Interestingly, XEG5B is, to date, the only GH5 member able to cleave XXXG into XX and XG, and in the light of these results, we propose that a modification in the 1 subsite enables the accommodation of a xylosyl side chain at this position. The stereochemical compatibility of the -1 subsite with a xylosyl moiety was also reported for other Structurally nonrelated XEGs belonging to the GH74 family, indicating it to be an essential attribute for this mode of action. C1 [dos Santos, Camila Ramos; Cordeiro, Rosa Lorizolla; Murakami, Mario Tyago] Natl Ctr Res Energy & Mat, Brazilian Biosci Natl Lab, BR-13083970 Campinas, SP, Brazil. [Wong, Dominic W. S.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Murakami, MT (reprint author), Brazilian Ctr Res Energy & Mat, Brazilian Biosci Natl Lab, Giuseppe Maximo Scolfaro 10000, BR-13083970 Campinas, SP, Brazil. EM mario.murakami@lnbio.cnpem.br OI Murakami, Mario/0000-0002-0405-8010 FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/51890-8, 13/13309-0]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [476043/2011-5, 308092/2012-0]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) FX This research was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [Grant Nos. 10/51890-8 and 13/13309-0 to M.T.M.], Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [Grant Numbers 476043/2011-5 and 308092/2012-0 to M.T.M.], and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES). NR 63 TC 2 Z9 2 U1 1 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 17 PY 2015 VL 54 IS 10 BP 1930 EP 1942 DI 10.1021/acs.biochem.5b00011 PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CD8EU UT WOS:000351328800008 PM 25714929 ER PT J AU Ma, H Weber, GM Hostuttler, MA Wei, HR Wang, L Yao, JB AF Ma, Hao Weber, Gregory M. Hostuttler, Mark A. Wei, Hairong Wang, Lei Yao, Jianbo TI MicroRNA expression profiles from eggs of different qualities associated with post-ovulatory ageing in rainbow trout (Oncorhynchus mykiss) SO BMC GENOMICS LA English DT Article DE microRNA; Egg quality; Post ovulation; Rainbow trout ID REPRODUCTIVE-PERFORMANCE; SALMO-GAIRDNERI; 1ST CLEAVAGE; CELL-DEATH; FISH; TRANSCRIPTOME; OVULATION; SURVIVAL; STRESS; GROWTH AB Background: Egg quality is an important aspect in rainbow trout farming. Post-ovulatory aging is one of the most important factors affecting egg quality. MicroRNAs (miRNAs) are the major regulators in various biological processes and their expression profiles could serve as reliable biomarkers for various pathological and physiological conditions. The objective of this study was to identify miRNAs that are associated with egg qualities in rainbow trout using post-ovulatory aged eggs. Results: Egg samples from females on day 1, day 7, and day 14 post-ovulation (D1PO, D7PO and D14PO), which had the fertilization rates of 91.8%, 73.4% and less than 50%, respectively, were collected and small RNAs isolated from these samples were subjected to deep sequencing using the Illumina platform. The massive sequencing produced 27,342,477, 26,910,438 and 29,185,371 reads from the libraries of D1PO, D7PO and D14PO eggs, respectively. A three-way comparison of the miRNAs indicated that the egg samples shared 392 known and 236 novel miRNAs, and a total of 414, 481, and 470 known and 243, 298, and 296 novel miRNAs were identified from D1PO, D7PO and D14PO eggs, respectively. Four known miRNAs (omy-miR-193b-3p, omy-miR-203c-3p, omy-miR-499-5p and omy-miR-7550-3p) and two novel miRNAs (omy-miR-nov-95-5p and omy-miR-nov-112-5p) showed significantly higher expression in D1PO eggs relative to D14PO eggs as revealed by both deep sequencing and real time quantitative PCR analysis. GO analysis of the predicted target genes of these differentially expressed miRNAs revealed significantly enriched GO terms that are related to stress response, cell death, DNA damage, ATP generation, signal transduction and transcription regulation. Conclusions: Results indicate that post-ovulatory ageing affects miRNA expression profiles in rainbow trout eggs, which can in turn impact egg quality. Further characterization of the differentially expressed miRNAs and their target genes may provide valuable information on the role of these miRNAs in controlling egg quality, and ultimately lead to the development of biomarkers for prediction of egg quality in rainbow trout. C1 [Ma, Hao; Wang, Lei; Yao, Jianbo] W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. [Weber, Gregory M.; Hostuttler, Mark A.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Wei, Hairong] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. RP Yao, JB (reprint author), W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. EM jianbo.yao@mail.wvu.edu FU USDA-ARS [58-1930-0-059] FX This project was supported by the USDA-ARS Cooperative Agreement No. 58-1930-0-059. It is published with the approval of the director of the West Virginia Agricultural and Forestry Experiment Station as scientific paper No. 3235. NR 68 TC 9 Z9 11 U1 2 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 17 PY 2015 VL 16 AR 201 DI 10.1186/s12864-015-1400-0 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CE3KZ UT WOS:000351726500001 PM 25885637 ER PT J AU Dhillon, B Feau, N Aerts, AL Beauseigle, S Bernier, L Copeland, A Foster, A Gill, N Henrissat, B Herath, P LaButti, KM Levasseur, A Lindquist, EA Majoor, E Ohm, RA Pangilinan, JL Pribowo, A Saddler, JN Sakalidis, ML de Vries, RP Grigoriev, IV Goodwin, SB Tanguay, P Hamelin, RC AF Dhillon, Braham Feau, Nicolas Aerts, Andrea L. Beauseigle, Stephanie Bernier, Louis Copeland, Alex Foster, Adam Gill, Navdeep Henrissat, Bernard Herath, Padmini LaButti, Kurt M. Levasseur, Anthony Lindquist, Erika A. Majoor, Eline Ohm, Robin A. Pangilinan, Jasmyn L. Pribowo, Amadeus Saddler, John N. Sakalidis, Monique L. de Vries, Ronald P. Grigoriev, Igor V. Goodwin, Stephen B. Tanguay, Philippe Hamelin, Richard C. TI Horizontal gene transfer and gene dosage drives adaptation to wood colonization in a tree pathogen SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE poplar pathogen; tree disease; fungal genomics; Septoria canker ID SEPTORIA-MUSIVA; POPLAR CLONES; LEAF-SPOT; HYBRID POPLAR; FOREST TREES; FUNGI; CHAETOGLOBOSINS; DOMESTICATION; POPULICOLA; EXPANSION AB Some of the most damaging tree pathogens can attack woody stems, causing lesions (cankers) that may be lethal. To identify the genomic determinants of wood colonization leading to canker formation, we sequenced the genomes of the poplar canker pathogen, Mycosphaerella populorum, and the closely related poplar leaf pathogen, M. populicola. A secondary metabolite cluster unique to M. populorum is fully activated following induction by poplar wood and leaves. In addition, genes encoding hemicellulose-degrading enzymes, peptidases, and metabolite transporters were more abundant and were up-regulated in M. populorum growing on poplar wood-chip medium compared with M. populicola. The secondary gene cluster and several of the carbohydrate degradation genes have the signature of horizontal transfer from ascomycete fungi associated with wood decay and from prokaryotes. Acquisition and maintenance of the gene battery necessary for growth in woody tissues and gene dosage resulting in gene expression reconfiguration appear to be responsible for the adaptation of M. populorum to infect, colonize, and cause mortality on poplar woody stems. C1 [Dhillon, Braham; Feau, Nicolas; Beauseigle, Stephanie; Herath, Padmini; Sakalidis, Monique L.; Hamelin, Richard C.] Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada. [Aerts, Andrea L.; Copeland, Alex; LaButti, Kurt M.; Lindquist, Erika A.; Ohm, Robin A.; Pangilinan, Jasmyn L.; Grigoriev, Igor V.] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. [Bernier, Louis] Univ Laval, Ctr Etud Foret, Quebec City, PQ G1V 0A6, Canada. [Foster, Adam; Tanguay, Philippe; Hamelin, Richard C.] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Quebec City, PQ G1V 4C7, Canada. [Gill, Navdeep] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. [Henrissat, Bernard] Aix Marseille Univ, UMR Ctr Natl Rech Sci 7257, F-13288 Marseille, France. [Henrissat, Bernard] King Abdulaziz Univ, Dept Biol Sci, Jeddah 21413, Saudi Arabia. [Levasseur, Anthony] Aix Marseille Univ, IHU Mediterranee Infect, INSERM U1095, URMITE,UM63,CNRS 7278,IRD 198, F-13005 Marseille, France. [Majoor, Eline; de Vries, Ronald P.] Royal Netherlands Acad Arts & Sci, Fungal Biodivers Ctr CBS KNAW, Cent Bur Schimmelcultures, Fungal Physiol, NL-3584 CT Utrecht, Netherlands. [Majoor, Eline; de Vries, Ronald P.] Univ Utrecht, Fungal Mol Physiol, NL-3584 CT Utrecht, Netherlands. [Pribowo, Amadeus; Saddler, John N.] Univ British Columbia, Forest Prod Biotechnol & Bioenergy, Vancouver, BC V6T 1Z4, Canada. [Goodwin, Stephen B.] Purdue Univ, ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Feau, N (reprint author), Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada. EM feaunico@mail.ubc.ca; Richard.Hamelin@RNCan-NRCan.gc.ca RI Sakalidis, Monique/D-5126-2011; Ohm, Robin/I-6689-2016; de Vries, Ronald/F-8125-2011; Fac Sci, KAU, Biol Sci Dept/L-4228-2013; OI Sakalidis, Monique/0000-0001-6251-7724; de Vries, Ronald/0000-0002-4363-1123; Goodwin, Stephen/0000-0001-5708-9729; Bernier, Louis/0000-0002-1789-8190 FU Genomic Research and Development Initiative of Natural Resources Canada; Genome Canada; Genome BC Project [2112]; Office of Science of the US Department of Energy [DE-AC02-05CH11231]; Netherlands Organization for Scientific Research; Netherlands Genomics Initiative [93511035] FX This work was supported by the Genomic Research and Development Initiative of Natural Resources Canada, Genome Canada, and Genome BC Project 2112. The work conducted by the US Department of Energy Joint Genome Institute was supported by the Office of Science of the US Department of Energy under Contract DE-AC02-05CH11231. E.M. was supported by a grant from The Netherlands Organization for Scientific Research and The Netherlands Genomics Initiative 93511035 (to R.P.d.V.). NR 43 TC 7 Z9 7 U1 8 U2 41 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 17 PY 2015 VL 112 IS 11 BP 3451 EP 3456 DI 10.1073/pnas.1424293112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD4NM UT WOS:000351060000081 PM 25733908 ER PT J AU Zhou, XJ Welsch, R Yang, Y Alvarez, D Riediger, M Yuan, H Fish, T Liu, JP Thannhauser, TW Li, L AF Zhou, Xiangjun Welsch, Ralf Yang, Yong Alvarez, Daniel Riediger, Matthias Yuan, Hui Fish, Tara Liu, Jiping Thannhauser, Theodore W. Li, Li TI Arabidopsis OR proteins are the major posttranscriptional regulators of phytoene synthase in controlling carotenoid biosynthesis SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE carotenoid; phytoene synthase; Arabidopsis; OR; posttranscriptional regulation ID BETA-CAROTENE; POTATO-TUBERS; PARTIAL-PURIFICATION; RIPENING PROCESSES; STRESS TOLERANCE; ORANGE GENE; ACCUMULATION; TOMATO; EXPRESSION; CAULIFLOWER AB Carotenoids are indispensable natural pigments to plants and humans. Phytoene synthase (PSY), the rate-limiting enzyme in the carotenoid biosynthetic pathway, and ORANGE (OR), a regulator of chromoplast differentiation and enhancer of carotenoid biosynthesis, represent two key proteins that control carotenoid biosynthesis and accumulation in plants. However, little is known about the mechanisms underlying their posttranscriptional regulation. Here we report that PSY and OR family proteins [Arabidopsis thaliana OR (AtOR) and AtOR-like] physically interacted with each other in plastids. We found that alteration of OR expression in Arabidopsis exerted minimal effect on PSY transcript abundance. However, overexpression of AtOR significantly increased the amount of enzymatically active PSY, whereas an ator ator-like double mutant exhibited a dramatically reduced PSY level. The results indicate that the OR proteins serve as the major posttranscriptional regulators of PSY. The ator or ator-like single mutant had little effect on PSY protein levels, which involves a compensatory mechanism and suggests partial functional redundancy. In addition, modification of PSY expression resulted in altered AtOR protein levels, corroborating a mutual regulation of PSY and OR. Carotenoid content showed a correlated change with OR-mediated PSY level, demonstrating the function of OR in controlling carotenoid biosynthesis by regulating PSY. Our findings reveal a novel mechanism by which carotenoid biosynthesis is controlled via posttranscriptional regulation of PSY in plants. C1 [Zhou, Xiangjun; Yang, Yong; Yuan, Hui; Fish, Tara; Liu, Jiping; Thannhauser, Theodore W.; Li, Li] ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14850 USA. [Zhou, Xiangjun; Yuan, Hui; Li, Li] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Welsch, Ralf; Alvarez, Daniel; Riediger, Matthias] Univ Freiburg, Fac Biol 2, D-79104 Freiburg, Germany. RP Welsch, R (reprint author), Univ Freiburg, Fac Biol 2, D-79104 Freiburg, Germany. EM ralf.welsch@biologie.uni-freiburg.de; ll37@cornell.edu RI Yuan, Hui/H-6723-2016; OI Welsch, Ralf/0000-0002-2865-2743 FU Deutsche Forschungsgemeinschaft [WE 4731/2-1] FX We are grateful to Manuel Rodriguez-Concepcion for his critical comments and suggestions to improve the manuscript, J. Kudla for providing pSPYCE(M) and pSPYNE173 vectors, and S. Lu and the Arabidopsis Biological Resource Center for Arabidopsis T-DNA insertion seeds. R.W. was funded by Grant WE 4731/2-1 from the Deutsche Forschungsgemeinschaft. NR 51 TC 25 Z9 25 U1 4 U2 39 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 17 PY 2015 VL 112 IS 11 BP 3558 EP 3563 DI 10.1073/pnas.1420831112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD4NM UT WOS:000351060000099 PM 25675505 ER PT J AU Marini, JC Didelija, IC AF Marini, Juan C. Didelija, Inka Cajo TI Arginine Depletion by Arginine Deiminase Does Not Affect Whole Protein Metabolism or Muscle Fractional Protein Synthesis Rate in Mice SO PLOS ONE LA English DT Article ID CANCER CACHEXIA; SKELETAL-MUSCLE; CITRULLINE SYNTHESIS; CONTROLLED-TRIAL; DEGRADATION; GLUTAMINE; SUPPLEMENTATION; THERAPY; ENZYMES; BODY AB Due to the absolute need for arginine that certain cancer cells have, arginine depletion is a therapy in clinical trials to treat several types of cancers. Arginine is an amino acids utilized not only as a precursor for other important molecules, but also for protein synthesis. Because arginine depletion can potentially exacerbate the progressive loss of body weight, and especially lean body mass, in cancer patients we determined the effect of arginine depletion by pegylated arginine deiminase (ADI-PEG 20) on whole body protein synthesis and fractional protein synthesis rate in multiple tissues of mice. ADI-PEG 20 successfully depleted circulating arginine (< 1 mu mol/L), and increased citrulline concentration more than tenfold. Body weight and body composition, however, were not affected by ADI-PEG 20. Despite the depletion of arginine, whole body protein synthesis and breakdown were maintained in the ADI-PEG 20 treated mice. The fractional protein synthesis rate of muscle was also not affected by arginine depletion. Most tissues (liver, kidney, spleen, heart, lungs, stomach, small and large intestine, pancreas) were able to maintain their fractional protein synthesis rate; however, the fractional protein synthesis rate of brain, thymus and testicles was reduced due to the ADI-PEG 20 treatment. Furthermore, these results were confirmed by the incorporation of ureido [C-14] citrulline, which indicate the local conversion into arginine, into protein. In conclusion, the intracellular recycling pathway of citrulline is able to provide enough arginine to maintain protein synthesis rate and prevent the loss of lean body mass and body weight. C1 [Marini, Juan C.] Baylor Coll Med, Dept Pediat, Sect Crit Care Med, Houston, TX 77030 USA. [Marini, Juan C.; Didelija, Inka Cajo] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Marini, JC (reprint author), Baylor Coll Med, Dept Pediat, Sect Crit Care Med, Houston, TX 77030 USA. EM marini@bcm.edu FU federal funds from the United States Department of Agriculture, Agricultural Research Service under Cooperative Agreement [58-6250-6-001]; National Institutes of Health, National Institute of General Medical Sciences [GM108940] FX This work was supported by federal funds from the United States Department of Agriculture, Agricultural Research Service under Cooperative Agreement Number 58-6250-6-001 (http://www.ars.usda.gov/main/main.htm) and the National Institutes of Health, National Institute of General Medical Sciences (GM108940; http://www.nigms.nih.gov/Pages/default.aspx). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 36 TC 3 Z9 3 U1 1 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 16 PY 2015 VL 10 IS 3 AR e0119801 DI 10.1371/journal.pone.0119801 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD6EY UT WOS:000351183500125 PM 25775142 ER PT J AU O'Donnell, KM Thompson, FR Semlitsch, RD AF O'Donnell, Katherine M. Thompson, Frank R., III Semlitsch, Raymond D. TI Partitioning Detectability Components in Populations Subject to Within-Season Temporary Emigration Using Binomial Mixture Models SO PLOS ONE LA English DT Article ID CAPTURE-RECAPTURE DESIGN; RED-BACKED SALAMANDER; TERRESTRIAL SALAMANDERS; DETECTION PROBABILITIES; ESTIMATING ABUNDANCE; PLETHODON-CINEREUS; IMPERFECT DETECTION; ECOLOGY; COUNTS; METAPOPULATION AB Detectability of individual animals is highly variable and nearly always < 1; imperfect detection must be accounted for to reliably estimate population sizes and trends. Hierarchical models can simultaneously estimate abundance and effective detection probability, but there are several different mechanisms that cause variation in detectability. Neglecting temporary emigration can lead to biased population estimates because availability and conditional detection probability are confounded. In this study, we extend previous hierarchical binomial mixture models to account for multiple sources of variation in detectability. The state process of the hierarchical model describes ecological mechanisms that generate spatial and temporal patterns in abundance, while the observation model accounts for the imperfect nature of counting individuals due to temporary emigration and false absences. We illustrate our model's potential advantages, including the allowance of temporary emigration between sampling periods, with a case study of southern red-backed salamanders Plethodon serratus. We fit our model and a standard binomial mixture model to counts of terrestrial salamanders surveyed at 40 sites during 3-5 surveys each spring and fall 2010-2012. Our models generated similar parameter estimates to standard binomial mixture models. Aspect was the best predictor of salamander abundance in our case study; abundance increased as aspect became more northeasterly. Increased time-since-rainfall strongly decreased salamander surface activity (i.e. availability for sampling), while higher amounts of woody cover objects and rocks increased conditional detection probability (i.e. probability of capture, given an animal is exposed to sampling). By explicitly accounting for both components of detectability, we increased congruence between our statistical modeling and our ecological understanding of the system. We stress the importance of choosing survey locations and protocols that maximize species availability and conditional detection probability to increase population parameter estimate reliability. C1 [O'Donnell, Katherine M.; Semlitsch, Raymond D.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] US Forest Serv, USDA, No Res Stn, Columbia, MO USA. RP O'Donnell, KM (reprint author), Univ Missouri, Div Biol Sci, 105 Tucker Hall, Columbia, MO 65211 USA. EM odonnell.katie.m@gmail.com FU GAANN Fellowship; U.S. Forest Service [09-JV-11242311-064] FX This project was funded by GAANN Fellowship (KMO) and U.S. Forest Service, Joint Venture Agreement 09-JV-11242311-064. U.S. Forest Service was responsible for overall study design, but was not involved in data collection or analysis, decision to publish, or preparation of the manuscript. NR 56 TC 6 Z9 6 U1 5 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 16 PY 2015 VL 10 IS 3 AR e0117216 DI 10.1371/journal.pone.0117216 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD6EY UT WOS:000351183500017 PM 25775182 ER PT J AU Yao, JL Kula, RR Chen, JH AF Yao, Junli Kula, Robert R. Chen, Jiahua TI A new species in the newly recorded genus Trachyusa (Hymenoptera: Braconidae: Alysiinae) from China SO ZOOTAXA LA English DT Article DE Asia; distribution; identification; key; parasitoid; taxonomy ID REVISION AB Trachyusa whartoni Yao sp. nov. from Dalian, Liaoning, China is described and illustrated. Trachyusa is a newly recorded genus in China. A key to the species of Trachyusa is provided based on the keys of Belokobylskij (1998), Papp (1967), and van Achterberg and O'Connor (1990). Type specimens of the new species are deposited in the Beneficial Insects Institute, Fujian Agriculture and Forestry University, Fuzhou, China. C1 [Yao, Junli; Chen, Jiahua] Fujian Agr & Forestry Univ, Beneficial Insects Inst, Fuzhou 350002, Fujian, Peoples R China. [Kula, Robert R.] Smithsonian Inst, Natl Museum Nat Hist, Systemat Entomol Lab, Beltsville Agr Res Ctr,ARS USDA, Washington, DC 20013 USA. RP Chen, JH (reprint author), Fujian Agr & Forestry Univ, Beneficial Insects Inst, Fuzhou 350002, Fujian, Peoples R China. EM yao.junli.china@gmail.com; Robert.Kula@ars.usda.gov; JhChen34@163.com FU Specialized Research Fund for the Doctoral Program in Colleges and Universities [20113515110003] FX We express our gratitude to Dr. Robert A. Wharton (Texas A&M University, USA) for his kindness in offering valuable reference material and specimens of Trachyusa while the first author visited Texas A& M University. We thank Dr. Dicky Yu for providing many references. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. This research was supported by the Specialized Research Fund for the Doctoral Program in Colleges and Universities (2011) for the project Systemic Studies on Alysiinae of China (code: 20113515110003). NR 23 TC 0 Z9 0 U1 0 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 16 PY 2015 VL 3931 IS 4 BP 579 EP 584 PG 6 WC Zoology SC Zoology GA CD5WH UT WOS:000351158800007 PM 25781847 ER PT J AU Haack, B Mahabir, R Kerkering, J AF Haack, Barry Mahabir, Ron Kerkering, John TI Remote sensing-derived national land cover land use maps: a comparison for Malawi SO GEOCARTO INTERNATIONAL LA English DT Article DE Malawi; land cover; IPCC; land use; greenhouse gases ID ACCURACY ASSESSMENT; SATELLITE DATA; CLASSIFICATION ACCURACY; CLIMATE; URBANIZATION; CHALLENGES; IMAGERY AB Reliable land cover land use (LCLU) information, and change over time, is important for Green House Gas (GHG) reporting for climate change documentation. Four different organizations have independently created LCLU maps from 2010 satellite imagery for Malawi for GHG reporting. This analysis compares the procedures and results for those four activities. Four different classification methods were employed; traditional visual interpretation, segmentation and visual labelling, digital clustering with visual identification and supervised signature extraction with application of a decision rule followed by analyst editing. One effort did not report classification accuracy and the other three had very similar and excellent overall thematic accuracies ranging from 85 to 89%. However, despite these high thematic accuracies there were very significant differences in results. National percentages for forest ranged from 18.2 to 28.7% and cropland from 40.5 to 53.7%. These significant differences are concerns for both remote-sensing scientists and decision-makers in Malawi. C1 [Haack, Barry; Mahabir, Ron] George Mason Univ, Dept Geog & Geoinformat Sci, Fairfax, VA 22030 USA. [Kerkering, John] US Forest Serv, Washington, DC 20250 USA. RP Mahabir, R (reprint author), George Mason Univ, Dept Geog & Geoinformat Sci, Fairfax, VA 22030 USA. EM rmahabir@gmu.edu NR 63 TC 4 Z9 4 U1 2 U2 10 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1010-6049 EI 1752-0762 J9 GEOCARTO INT JI Geocarto Int. PD MAR 16 PY 2015 VL 30 IS 3 BP 270 EP 292 DI 10.1080/10106049.2014.952355 PG 23 WC Environmental Sciences; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Geology; Remote Sensing; Imaging Science & Photographic Technology GA CD0CQ UT WOS:000350738300003 ER PT J AU Lulai, EC Neubauer, JD Olson, LL Suttle, JC AF Lulai, Edward C. Neubauer, Jonathan D. Olson, Linda L. Suttle, Jeffrey C. TI Wounding induces changes in tuber polyamine content, polyamine metabolic gene expression, and enzyme activity during closing layer formation and initiation of wound periderm formation SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Arginine decarboxylase; Ornithine decarboxylase; Potato; Wound-heal; Wound-periderm ID POTATO-TUBER; SOLANUM-TUBEROSUM; INDUCED SUBERIZATION; PHENYLPROPANOID METABOLISM; BIOSYNTHESIS; INVOLVEMENT; OXIDASE; STRESS; ARABIDOPSIS; PROGRESSION AB Tuber wound-healing processes are complex, and the associated regulation and modulation of these processes are poorly understood. Polyamines (PA) are involved in modulating a variety of responses to biotic and abiotic plant stresses and have been suggested to be involved in tuber wound responses. However, the time course of wound-induced changes in tuber PA content, activity of key biosynthetic enzymes and associated gene expression has not been determined and coordinated with major wound-healing processes. The objective of this study was to determine these wound-induced changes and their coordination with wound-healing processes. Wounding induced increases in putrescine (Put) and spermidine (Spd), but had only minor effects on spermine (Spm) content during the 168 h time course which encompassed the initiation and completion of the closing layer formation, and the initiation of cell division and wound periderm formation. As determinants of the first committed step in PA biosynthesis, arginine and ornithine decarboxylase (ADC and ODC, respectively) activities were below levels of detectability in resting tubers and expression of genes encoding these two enzymes was low. Within 6 h of wounding, increases in the in vitro activities of ADC and ODC and expression of their cognate genes were observed. Expression of a gene encoding S-adenosylmethionine decarboxylase, required for Spd and Spm biosynthesis, was also increased 6 h after wounding and remained elevated throughout the time course. Expression of a polyamine catabolic gene, encoding polyamine oxidase, was down-regulated after wounding. Results indicated a rapid wound-induced increase in PA biosynthesis during closing layer formation and the time of nuclei entry and exit from S-phase. PA content remained elevated as wound-induced cells became meristematic and initiated formation of the wound periderm suggesting sustained involvement in wound-healing. Published by Elsevier GmbH. C1 [Lulai, Edward C.; Neubauer, Jonathan D.; Olson, Linda L.; Suttle, Jeffrey C.] ARS, USDA, Sugarbeet & Potato Unit, NCSL, Fargo, ND 58102 USA. RP Lulai, EC (reprint author), ARS, USDA, NCSL, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM ed.lulai@ars.usda.gov NR 41 TC 4 Z9 4 U1 7 U2 36 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD MAR 15 PY 2015 VL 176 BP 89 EP 95 DI 10.1016/j.jplph.2014.12.010 PG 7 WC Plant Sciences SC Plant Sciences GA CE9GD UT WOS:000352151100010 PM 25577734 ER PT J AU Houborg, R McCabe, M Cescatti, A Gao, F Schull, M Gitelson, A AF Houborg, Rasmus McCabe, Matthew Cescatti, Alessandro Gao, Feng Schull, Mitchell Gitelson, Anatoly TI Joint leaf chlorophyll content and leaf area index retrieval from Landsat data using a regularized model inversion system (REGFLEC) SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat; Leaf chlorophyll; LAI; SAIL; PROSPECT; Canopy reflectance; Model inversion ID RADIATIVE-TRANSFER MODELS; REMOTE-SENSING DATA; VEGETATION BIOPHYSICAL PARAMETERS; GROSS PRIMARY PRODUCTION; HIGHER-PLANT LEAVES; CANOPY REFLECTANCE; SURFACE REFLECTANCE; NITROGEN-CONTENT; NONDESTRUCTIVE ESTIMATION; PHOTOSYNTHETIC CAPACITY AB Leaf area index (LAI) and leaf chlorophyll content (Chl(l)) represent key biophysical and biochemical controls on water, energy and carbon exchange processes in the terrestrial biosphere. In combination, [LAI and Chi(1), provide critical information on vegetation density, vitality and photosynthetic potentials. However, simultaneous retrieval of LAI and Chl(l) from space observations is extremely challenging. Regularization strategies are required to increase the robustness and accuracy of retrieved properties and enable more reliable separation of soil, leaf and canopy parameters. To address these challenges, the REGularized canopy reFLECtance model (REGFLEC) inversion system was refined to incorporate enhanced techniques for exploiting ancillary LAI and temporal information derived from multiple satellite scenes. In this current analysis, REGFLEC is applied to a time-series of Landsat data. A novel aspect of the REGFLEC approach is the fact that no site-specific data are required to calibrate the model, which may be run in a largely automated fashion using information extracted entirely from image-based and other widely available datasets. Validation results, based upon in-situ LAI and Chl(l) observations collected over maize and soybean fields in central Nebraska for the period 2001-2005, demonstrate Chl(l) retrieval with a relative root-mean-square-deviation (RMSD) on the order of 19% (RMSD = 8.42 pg cm(-2)). While Chli retrievals were clearly influenced by the version of the leaf optical properties model used (PROSPECT), the application of spatio-temporal regularization constraints was shown to be critical for estimating Chl(l) with sufficient accuracy. REGFLEC also reproduced the dynamics of in-situ measured LAI well (r(2) = 0.85), but estimates were biased low, particularly over maize (LAI was underestimated by -36 %). This disparity may be attributed to differences between effective and true LAI caused by significant foliage clumping not being properly accounted for in the canopy reflectance model (SAIL). Additional advances in the retrieval of canopy biophysical and leaf biochemical constituents will require innovative use of existing remote sensing data within physically realistic canopy reflectance models along with the ability to exploit the enhanced spectral and spatial capabilities of upcoming satellite systems. (C) 2014 Elsevier Inc. All rights reserved. C1 [Houborg, Rasmus; McCabe, Matthew] King Abdullah Univ Sci & Technol, Water Desalinat & Reuse Ctr, Thuwal, Saudi Arabia. [Cescatti, Alessandro] European Commiss, Inst Environm & Sustainabil, Joint Res Ctr, Ispra, Italy. [Gao, Feng; Schull, Mitchell] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Gitelson, Anatoly] Univ Nebraska, Sch Nat Resources, Ctr Adv Land Management Informat Technol, Lincoln, NE USA. RP Houborg, R (reprint author), King Abdullah Univ Sci & Technol, Environm Sci & Engn Div, Water Desalinat & Reuse Ctr, Thuwal, Saudi Arabia. EM rasmus.houborg@kaust.edu.sa RI McCabe, Matthew/G-5194-2011 OI McCabe, Matthew/0000-0002-1279-5272 FU King Abdullah University of Science and Technology (KAUST); Marie Curie International Incoming Fellowship FX The research undertaken here was funded by the King Abdullah University of Science and Technology (KAUST). We appreciate the data provided by the Center for Advanced Land Management Information Technologies (CALMIT) and the Carbon Sequestration Program, University of Nebraska-Lincoln. Anatoly Gitelson acknowledge support of Marie Curie International Incoming Fellowship. NR 128 TC 14 Z9 14 U1 2 U2 41 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 15 PY 2015 VL 159 BP 203 EP 221 DI 10.1016/j.rse.2014.12.008 PG 19 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CF7PO UT WOS:000352749000016 ER PT J AU Ramanathan, P Zhu, JJ Bishop, EA Puckette, MC Hartwig, E Grubman, MJ Rodriguez, LL AF Ramanathan, Palaniappan Zhu, James J. Bishop, Elizabeth A. Puckette, Michael C. Hartwig, Ethan Grubman, Marvin J. Rodriguez, Luis L. TI A colorimetric bioassay for high-throughput and cost-effectively assessing anti-foot-and-mouth disease virus activity SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Foot-and-mouth disease virus; Type I interferons; Antiviral activity; Cytopathic effect reduction assay ID KIDNEY-CELL-LINE; ALPHA/BETA INTERFERON; SUSCEPTIBILITY; ASSAYS; REPLICATION; VALIDATION; CULTURE AB Foot-and-mouth disease virus (FMDV) is one of the most contagious animal viruses. This virus is very sensitive to inhibition by type I interferons. Currently, a bioassay based on plaque reduction is used to measure anti-FMDV activity of porcine IFNs. The plaque reduction assay is tedious and difficult to utilize for high-throughput analysis. Using available FMDV susceptible bovine and porcine cells, we developed and tested a colorimetric assay based on cytopathic effect reduction for its ability to quantify FMDV-specific antiviral activity of bovine and porcine type I interferons. Our results show that this new method has significant advantages over other assays in terms of labor intensity, cost, high-throughput capability and/or anti-FMDV specific activity because of simpler procedures and direct measurement of antiviral activity. Several assay conditions were tested to optimize the procedures. The test results show that the assay can be standardized with fixed conditions and a standard or a reference for measuring antiviral activity as units. This is an excellent assay in terms of sensitivity and accuracy based on a statistical evaluation. The results obtained with this assay were highly correlated with a conventional virus titration method. Published by Elsevier B.V. C1 [Ramanathan, Palaniappan; Zhu, James J.; Bishop, Elizabeth A.; Puckette, Michael C.; Hartwig, Ethan; Grubman, Marvin J.; Rodriguez, Luis L.] USDA, Foreign Anim Dis Res Unit, Agr Res Unit, Plum Isl Anim Dis Res Ctr, Orient Point, NY 11957 USA. RP Zhu, JJ (reprint author), USDA ARS, FADRU, NAA, Plum Isl Anim Dis Ctr, Orient Point, NY 11957 USA. EM james.zhu@ars.usda.gov; luis.rodriguez@ars.usda.gov RI Ramanathan, Palaniappan/B-1062-2012 OI Ramanathan, Palaniappan/0000-0001-8121-7647 FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-09-X-00373] FX The authors want to express our gratitude to Mr. Mike LaRocco and Ms. Marla Koster, FADRU, USDA-ARS, for providing the cell lines and Dr. Z. Lu, PIADC, DHS, for performing the DNA sequencing. This project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-09-X-00373. NR 13 TC 2 Z9 2 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD MAR 15 PY 2015 VL 164 IS 1-2 BP 74 EP 78 DI 10.1016/j.vetimm.2015.01.005 PG 5 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA CF0RD UT WOS:000352251000010 PM 25669593 ER PT J AU Ghane, E Fausey, NR Brown, LC AF Ghane, Ehsan Fausey, Norman R. Brown, Larry C. TI Modeling nitrate removal in a denitrification bed SO WATER RESEARCH LA English DT Article DE Arrhenius; Bromide tracer; Drainage water; Forchheimer; Greenhouse gas; Woodchip bioreactor ID DRAINAGE WATER MANAGEMENT; NITROUS-OXIDE; AGRICULTURAL DRAINAGE; METHANE EMISSIONS; BIOREACTORS; RATES; FIELD; PERFORMANCE; CALIBRATION; REDUCTION AB Denitrification beds are promoted to reduce nitrate load in agricultural subsurface drainage water to alleviate the adverse environmental effects associated with nitrate pollution of surface water. In this system, drainage water flows through a trench filled with a carbon media where nitrate is transformed into nitrogen gas under anaerobic conditions. The main objectives of this study were to model a denitrification bed treating drainage water and evaluate its adverse greenhouse gas emissions. Field experiments were conducted at an existing denitrification bed. Evaluations showed very low greenhouse gas emissions (mean N2O emission of 0.12 mu g N m(-2) min(-1)) from the denitrification bed surface. Field experiments indicated that nitrate removal rate was described by Michaelis-Menten kinetics with the Michaelis-Menten constant of 7.2 mg N L-1. We developed a novel denitrification bed model based on the governing equations for water flow and nitrate removal kinetics. The model evaluation statistics showed satisfactory prediction of bed outflow nitrate concentration during subsurface drainage flow. The model can be used to design denitrification beds with efficient nitrate removal which in turn leads to enhanced drainage water quality. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Ghane, Ehsan; Brown, Larry C.] Ohio State Univ, Dept Food Agr & Biol Engn, Columbus, OH 43210 USA. [Fausey, Norman R.] USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. RP Ghane, E (reprint author), Ohio State Univ, Dept Food Agr & Biol Engn, Columbus, OH 43210 USA. EM ehsan.ghane@outlook.com FU Ohio Agricultural and Research Development Center [2013-098]; Ohio State University Graduate School Presidential Fellowship; Overholt Drainage Education and Research Program; Department of Food, Agricultural and Biological Engineering; College of Food, Agricultural, and Environmental Sciences; USDA-Natural Resources Conservation Service CIG [69-5E34-10-053] FX Funding of this research was provided in part by Ohio Agricultural and Research Development Center grant number 2013-098; Ohio State University Graduate School Presidential Fellowship to lead author; Overholt Drainage Education and Research Program; Department of Food, Agricultural and Biological Engineering; College of Food, Agricultural, and Environmental Sciences; and USDA-Natural Resources Conservation Service CIG grant number 69-5E34-10-053. The funding sources were not involved in the study design, collection, analysis, interpretation of data, and preparation of the manuscript. NR 63 TC 13 Z9 13 U1 13 U2 53 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD MAR 15 PY 2015 VL 71 BP 294 EP 305 DI 10.1016/j.watres.2014.10.039 PG 12 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA CE6RX UT WOS:000351966900026 PM 25638338 ER PT J AU Liu, JD Linderholm, H Chen, DL Zhou, XJ Flerchinger, GN Yu, Q Du, J Wu, DR Shen, YB Yang, ZB AF Liu, Jiandong Linderholm, Hans Chen, Deliang Zhou, Xiuji Flerchinger, Gerald N. Yu, Qiang Du, Jun Wu, Dingrong Shen, Yanbo Yang, Zhenbin TI Changes in the relationship between solar radiation and sunshine duration in large cities of China SO ENERGY LA English DT Article DE Solar radiation; Sunshine duration; Angstrom model; Linear relationship; Calibration ID BRIGHT SUNSHINE; IRRADIANCE; ANGSTROM; COEFFICIENTS; POLLUTION; WEATHER; MODELS; CLOUDS; TRENDS AB Based on the linear relationship between solar radiation and sunshine duration, the Angstrom model is widely used to estimate solar radiation from routinely observed meteorological variables for energy exploitation. However, the relationship may have changed in quickly developing regions in the recent decades under global "dimming" and "brightening" context, with increasing aerosols due to industrial pollutions. Solar radiation stations under different climate conditions in six large cities in China are selected to test this hypothesis. Analysis of the related meteorological items shows that Guiyang has the lowest solar radiation with the average annual value of 10.5 MJm(-2)d(-1), while Lhasa on the Tibetan Plateau has the highest of 20.1 MJm(-2)d(-1). Both radiation and sunshine hours decreased from 1961 to 2010, but at different rates. A moving linear regression method is used to investigate the changes in the relationship between radiation and sunshine duration, the results indicate an abrupt change in the correlation coefficients in 1980-1990s, which can be attributed to the aerosol load resulting from air pollution caused by the industrial development in 1980s under China's Open Door Policy. The sky condition has been changing from clean to dirty, thus the relationship between solar radiation and duration changes in the 1980's and has recovered in the recent decades. This finding implies that it might not necessarily be right to use long data sets for model calibration. Further investigation confirms that the Angstrom model performs the best with higher NSE (nash-sutcliffe efficiency) of 0.914 and lower MAPE (mean absolute percentage error) and RMSE (root mean square error) values of 13.7 w/m(2) and 23.9 w/m(2) respectively, when calibrated with a 10-year data set. In contrast, the model performs worst when it is calibrated with a 40-year data set, with NSE, MAPE and RMSE values of 0.891, 15.1 w/m(2) and 25.3 w/m(2), respectively. Based on the findings of this research, a 10-year data set is recommended as the national standard for model calibration in rapidly developing regions of China. Further analogous investigations are needed in other industrial regions to make an international standard for Angstrom model calibration. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Liu, Jiandong; Zhou, Xiuji; Wu, Dingrong] Chinese Acad Meteorol Sci, Ctr Agrometeorol, China Meteorol Adm, Beijing 100081, Peoples R China. [Liu, Jiandong; Linderholm, Hans] Univ Gothenburg, Dept Earth Sci, Reg Climate Grp, Gothenburg, Sweden. [Chen, Deliang; Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Yu, Qiang] Univ Technol, Plant Funct & Climate Change Cluster, Sydney, NSW, Australia. [Du, Jun] Tibet Autonomous Meteorol Adm, Tibet Climate Ctr, Lhasa, Peoples R China. [Shen, Yanbo; Yang, Zhenbin] China Meteorol Adm, Ctr Solar & Wind Energy Res, Beijing, Peoples R China. RP Liu, JD (reprint author), Chinese Acad Meteorol Sci, Ctr Agrometeorol, 46 Zhongguancun South St, Beijing 100081, Peoples R China. EM liujd2001@263.net RI Chen, Deliang/A-5107-2013; Linderholm, Hans/N-1020-2013; Yu, Qiang/D-3702-2009 OI Chen, Deliang/0000-0003-0288-5618; Linderholm, Hans/0000-0002-1522-8919; Yu, Qiang/0000-0001-6950-1821 FU National Science Foundation of China [41105079]; Special Key Project on "Estimation and Prediction of the Solar Radiation in China" [GYHY201306048] FX This research is jointly supported by the National Science Foundation of China (No. 41105079) and Special Key Project on "Estimation and Prediction of the Solar Radiation in China" (No. GYHY201306048). The authors sincerely thank the anonymous referees for their strict but instructive comments on the manuscript. Special thanks are also extended to the weathermen at Lhasa station on the Tibetan Plateau under altitude hypoxia conditions. NR 42 TC 2 Z9 3 U1 2 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0360-5442 EI 1873-6785 J9 ENERGY JI Energy PD MAR 15 PY 2015 VL 82 BP 589 EP 600 DI 10.1016/j.energy.2015.01.068 PG 12 WC Thermodynamics; Energy & Fuels SC Thermodynamics; Energy & Fuels GA CE4GI UT WOS:000351788700053 ER PT J AU Benjankar, R Tonina, D McKean, J AF Benjankar, Rohan Tonina, Daniele McKean, James TI One-dimensional and two-dimensional hydrodynamic modeling derived flow properties: impacts on aquatic habitat quality predictions SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE one-dimensional and two-dimensional hydrodynamic models; stream morphology; habitat modeling; aquatic habitat quality distribution; longitudinal scales of topographical features ID SPAWNING HABITAT; STREAM HABITATS; KOOTENAI RIVER; 2D; SIMULATION; VELOCITY; USA; GEOMORPHOLOGY; SUITABILITY; INUNDATION AB Studies of the effects of hydrodynamic model dimensionality on simulated flow properties and derived quantities such as aquatic habitat quality are limited. It is important to close this knowledge gap especially now that entire river networks can be mapped at the microhabitat scale due to the advent of point-cloud techniques. This study compares flow properties, such as depth and velocity, and aquatic habitat quality predicted from pseudo-2D and fully 2D hydrodynamic modeling. The models are supported by high-resolution, point-cloud derived bathymetries, from which close-spaced cross-sections were extracted for the 1D modeling, of three morphologically and hydraulically different river systems. These systems range from small low-gradient meandering pool-riffle to large steep confined plane-bed rivers. We test the effects of 1D and 2D models on predicted hydraulic variables at cross-sections and over the full bathymetry to quantify the differences due to model dimensionality and those from interpolation. Results show that streambed features, whose size is smaller than cross-sectional spacing, chiefly determine the different results of 1D and 2D modeling whereas flow discharge, stream size, morphological complexity and model grid sizes have secondary effects on flow properties and habitat quality for a given species and life stage predicted from 1D and 2D modeling. In general, the differences in hydraulic variables are larger in the bathymetric than in the cross-sectional analysis, which suggests that some errors are introduced from interpolation of spatially disaggregated simulated variables with a 1D model, instead of model dimensionality 1D or 2D. Flow property differences are larger for velocity than for water surface elevation and depth. Differences in weighted usable area (WUA) derived from 1D and 2D modeling are relatively small for low-gradient meandering pool-riffle systems, but the differences in the spatial distribution of microhabitats can be considerable although clusters of same habitat quality are spatially comparable. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Benjankar, Rohan; Tonina, Daniele] Univ Idaho, Ctr Ecohydraul Res, Boise, ID 83702 USA. [McKean, James] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. RP Benjankar, R (reprint author), Univ Idaho, Ctr Ecohydraul Res, 322 E Front St,Suite 340, Boise, ID 83702 USA. EM rohanb@uidaho.edu RI Tonina, Daniele/I-7688-2012 OI Tonina, Daniele/0000-0002-1866-1013 FU United States Bureau of Reclamation [Rl2APJ 1025]; United States Forest Service [08-JV-11221659-036]; Center for Ecohydraulics Research (CER); University of Idaho (UI) Research Office FX We thank Dr C. Podolak, the editors and an anonymous reviewer for their constructive criticism and comments on an earlier version. Their suggestions greatly improved the manuscript. The study was partially supported by the United States Bureau of Reclamation grant Rl2APJ 1025 and the United States Forest Service grant 08-JV-11221659-036. Additional funding is provided by Center for Ecohydraulics Research (CER) and University of Idaho (UI) Research Office. We would like to thank the DHI (Danish Hydraulic Institute) for providing MIKE software packages to the University of Idaho. Any opinions, conclusions, or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of US Bureau of Reclamation or the US Forest Service. NR 80 TC 2 Z9 3 U1 0 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD MAR 15 PY 2015 VL 40 IS 3 BP 340 EP 356 DI 10.1002/esp.3637 PG 17 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CD4PW UT WOS:000351066900005 ER PT J AU Arthur, MA Blankenship, BA Schorgendorfer, A Loftis, DL Alexander, HD AF Arthur, Mary A. Blankenship, Beth A. Schoergendorfer, Angela Loftis, David L. Alexander, Heather D. TI Changes in stand structure and tree vigor with repeated prescribed fire in an Appalachian hardwood forest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Acer; Quercus; Sprouting; Prescribed fire; Stand structure; Mesophytic ID EASTERN UNITED-STATES; MIXED-OAK FORESTS; FUEL REDUCTION TREATMENTS; NORTH-AMERICA; CUMBERLAND PLATEAU; SOUTHERN APPALACHIANS; VEGETATION STRUCTURE; UPLAND OAK; REGENERATION; DYNAMICS AB Without large scale disturbances to alter forest structure and open the canopy, historically oak-dominated forests of the central and Appalachian hardwood regions of eastern North America are shifting to dominance by shade-tolerant, 'mesophytic' species. In response, prescribed fire is applied with increasing frequency and spatial extent to decrease non-oak species and promote dominance of oak species. However, relatively few studies have examined impacts of repeated fire to forest structure and tree vigor across multiple years and varied terrain. In this study, we examined tree vigor, tree mortality, and stand structure in response to different burn treatments: Frequent (burned 4 times in eight years), Less Frequent (burned 2 times in eight years), and Fire-Excluded. We hypothesized that fire-driven decreases in stem density and basal area would be greatest for small size classes, especially of shade-tolerant species on drier landscape positions, and would increase with burn frequency and fire temperature. We expected trees surviving fire to exhibit increased crown vigor over time since fire. Prescribed fire effects depended on tree size-class and landscape position. About 60% of surviving midstory trees (10-20 cm diameter at breast height (DBH)) and 25% of overstory trees (>= 20 cm DBH) on sub-xeric and intermediate landscape positions experienced crown dieback. Fire-Excluded sites had fewer trees with crown dieback (11-28% across size classes) compared to burned sites (21-87%). Throughout the duration of the study, midstory and overstory maples had significantly greater likelihood of increased crown dieback compared to oaks. Paradoxically, midstory maples had a higher survival probability than similarly-sized oaks, while overstory maples had lower survival than overstory oaks. The greatest reductions in density and basal area occurred in saplings (trees 2-10 cm DBH) and midstory trees on sub-xeric and intermediate (but not sub-mesic) landscape positions. Both Less Frequent and Frequent burning reduced density and basal area of sapling and mid-story shade-tolerant species, but also of mid-story chestnut oaks. Individual tree mortality was positively correlated with char height after the first burn regardless of burn frequency. A large and significant initial sprouting response to fire dissipated over time and with repeated burning. Future assessments of mortality and vigor of residual trees following fire are essential for evaluating the long-term effectiveness of prescribed fire management in shifting species composition away from 'mesophytic' species and toward oaks, and could help guide management choices regarding repeated prescribed burning. (C) 2015 Elsevier B.V. All rights reserved. C1 [Arthur, Mary A.; Blankenship, Beth A.; Schoergendorfer, Angela; Alexander, Heather D.] Univ Kentucky, Dept Forestry, Lexington, KY 40546 USA. [Schoergendorfer, Angela] IBM Corp, TJ Watson Res Ctr, Yorktown Hts, NY 10598 USA. [Loftis, David L.] USDA, Forest Serv Southern Res Stn, Bent Creek Expt Forest, Asheville, NC 28806 USA. [Alexander, Heather D.] Univ Texas Brownsville, Dept Biol Sci, Brownsville, TX 78520 USA. RP Arthur, MA (reprint author), Univ Kentucky, Dept Forestry, TP Cooper Bldg, Lexington, KY 40546 USA. EM marthur@uky.edu; beth.blankenship@uky.edu; angelasch@gmail.com; davidloftis@bellsouth.net; heather.alexander@utb.edu RI Clark, Stacy/I-3410-2015 FU Kentucky Agricultural Experiment Station [14-09-084]; USDA-USDI Joint Fire Science Program through USDA Forest Service Southern Research Station [01-3-3-14, 04-2-1-06]; USDA-USDI Joint Fire Science Program through Daniel Boone National Forest; University of Kentucky by the McIntire-Stennis Act FX This study (#14-09-084) is connected with a project of the Kentucky Agricultural Experiment Station. The research was supported by the USDA-USDI Joint Fire Science Program (01-3-3-14, 04-2-1-06) through a cooperative research agreement with the USDA Forest Service Southern Research Station, a Challenge Cost-Share Agreement with the Daniel Boone National Forest, and funding through the University of Kentucky provided by the McIntire-Stennis Act. We appreciate the contributions of many people, too numerous to mention individually, who supported this through field work, laboratory support, and elements of study design and site selection. Special thanks, however, are warranted to Autumn Foushee, Jessi Lyons, and Gretchen Carmean who led the field crews during many of the field seasons that led to the data presented here. We are also indebted to our USDA Forest Service collaborators from the Cumberland District of the Daniel Boone National Forest, including Jeff Lewis, EJ Bunzendahl, and David Manner. NR 64 TC 7 Z9 7 U1 8 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 15 PY 2015 VL 340 BP 46 EP 61 DI 10.1016/j.foreco.2014.12.025 PG 16 WC Forestry SC Forestry GA CD2SQ UT WOS:000350929700006 ER PT J AU Johnson, TN Buskirk, SW Hayward, GD Raphael, MG AF Johnson, Tracey N. Buskirk, Steven W. Hayward, Gregory D. Raphael, Martin G. TI Timber harvest interacts with broad-scale forest mortality to affect site occupancy dynamics of a vertebrate seed predator SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bark beetle; Colonization; Disturbance interactions; Extinction; Forest health; Subalpine forest ID YELLOWSTONE-NATIONAL-PARK; SUB-ALPINE FOREST; RED SQUIRRELS; LODGEPOLE PINE; DISTURBANCE INTERACTIONS; UNITED-STATES; SPOTTED OWLS; FOOD-HABITS; BARRED OWLS; ECOLOGY AB Extensive ecological disturbances can interact with spatial heterogeneity produced by previous disturbances, influencing responses of vertebrates to environmental change. Recent and ongoing outbreaks of bark beetles (including Dendroctonus spp. and Dryocoetes confuses) in the Rocky Mountains produced an opportunity to investigate effects of broad-scale tree mortality on an important seed predator in Nearctic coniferous forests, the red squirrel (Tamiasciurus hudsonicus), and to evaluate whether those effects interact with forest heterogeneity produced by previous timber harvest. Our study characterized site occupancy dynamics for red squirrels in relation to patch-cutting, a type of group-selection cut, and the bark-beetle outbreak, and evaluated whether patch cutting influenced subsequent effects of bark beetles on these squirrels. We used multi-season occupancy models and covariates for harvest- and outbreak-related habitat characteristics to describe extinction and colonization rates of red squirrels over a 27-yr period in Wyoming, USA. We observed effects of year, patch-cutting, and the bark beetle outbreak on the probability of detecting a red squirrel. We observed a negative association between local extinction rate and increasing snag density, but only to a threshold of 5 snags/0.04 ha. Local colonization rate was positively associated with the basal area of live trees. Annual site occupancy varied across years (range pre-harvest: 0.76-0.89; post-harvest: 0.84-0.99), and was lowest (0.70-0.72) during the two years sampling occurred approximately 14 years after the bark beetle outbreaks began. Tree mortality was lowest near patch cuts; this pattern was especially pronounced for mature trees (>30 cm diameter at breast height), which tend to produce the most cones and would likely contribute the most to red squirrel survival. Strong habitat effects on occupancy dynamics suggest that previously-harvested areas may provide refugia for red squirrels in post-outbreak forests. Our results support managing for uneven-aged stands of mixed species composition in subalpine forests of the Rocky Mountains. (C) 2014 Elsevier B.V. All rights reserved. C1 [Johnson, Tracey N.; Buskirk, Steven W.] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Hayward, Gregory D.] US Forest Serv, Anchorage, AK 99503 USA. [Raphael, Martin G.] US Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. RP Johnson, TN (reprint author), Western EcoSyst Technol Inc, 200 S 2nd St, Laramie, WY 82070 USA. EM tjohnson@west-inc.com RI Buskirk, Steven/K-2871-2016 OI Buskirk, Steven/0000-0003-0611-6648 FU U.S.D.A Forest Service [09-CS-11020600-043]; Wyoming Game and Fish Department's State Wildlife Grant Program, University of Wyoming; Wyoming Cooperative Fish and Wildlife Research Unit FX Support for this work provided by U.S.D.A Forest Service Cooperative Agreement Number 09-CS-11020600-043, Wyoming Game and Fish Department's State Wildlife Grant Program, University of Wyoming, and Wyoming Cooperative Fish and Wildlife Research Unit. S. Henry, S. Loose, and R. Skorkowsky assisted with project logistics. K. Dugger provided guidance on the statistical analysis, and D. Bell provided helpful comments on an earlier version of the manuscript. We thank the anonymous reviewers for constructive comments that improved the manuscript, and numerous field technicians for assistance with data collection. NR 53 TC 0 Z9 0 U1 5 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 15 PY 2015 VL 340 BP 95 EP 102 DI 10.1016/j.foreco.2014.12.030 PG 8 WC Forestry SC Forestry GA CD2SQ UT WOS:000350929700010 ER PT J AU Bormann, BT Darbyshire, RL Homann, PS Morrissette, BA Little, SN AF Bormann, Bernard T. Darbyshire, Robyn L. Homann, Peter S. Morrissette, Brett A. Little, Susan N. TI Managing early succession for biodiversity and long-term productivity of conifer forests in southwestern Oregon SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Early succession; Early-seral; Biodiversity; Long-term productivity; N-2 fixation ID DOUGLAS-FIR PLANTATIONS; WEED-CONTROL TREATMENTS; UNDERSTORY VEGETATION; COMPETING VEGETATION; SOUTHEASTERN ALASKA; PACIFIC-NORTHWEST; COASTAL OREGON; PONDEROSA PINE; UNITED-STATES; OLD-GROWTH AB Early-successional stages have been truncated and altered in many western U.S. forest landscapes by planting conifers, controlling competing vegetation, suppressing fire, and focusing on maintaining late-seral species and undisturbed riparian zones. Declining area of early-successional stages may be reducing resilience and sustainability on landscapes that experience elevated disturbance related to future climate changes. In this study, two post-harvest early-successional treatments were compared to each other and to two mature-forest treatments using 20 years of evidence from replicated 7-ha experimental units in a southwestern Oregon forest dominated by Douglas-fir (Pseudotsuga menziesii Mirb. Franco). One early-successional treatment (Douglas-fir plantation) planted Douglas-fir and was followed by a brushing to reduce hardwood competition to move quickly to the conifer stem-exclusion stage; the other (Early-seral plantation) favored natural sprouting and regeneration of hardwood shrubs and trees and planted scattered knobcone pines (Pinus attenuata Lemmon) and Douglas-fir. Plant diversity in the Early-seral plantation was 56% (year 2) and 26% (year 6) higher than in the Douglas-fir plantation. Both early-successional treatments far exceeded plant diversity in Unaltered and Thinned mature stands. Fifteen years of growth of shrubs and hardwood trees in the Early-seral plantation was remarkable, resulting in total aboveground biomass increment (18 Mg ha(-1) yr(-1)) double that of the Douglas-fir plantations. Important process effects related to primary productivity were noted: losses of soil organic matter from the B horizon in young Douglas-fir, and, after wildfire, increases in N-2-fixing plant cover in Early-seral plantation. The burl-sprouting and deep rooting of many hardwoods also created opportunities for nutrient retention and release from primary minerals as well as deep-profile water supply. Recognizing the importance of intentionally managing for shrubs and hardwood trees is particularly relevant at this site, because stand reconstruction and historical records indicate these species, along with knobcone pine, dominated the site for 40 years before the current mature Douglas-fir forest started gaining dominance. In contrast, the prolific natural regeneration of Douglas-fir after recent harvest and wildfire suggests that what comes back "naturally" in modern times will not allow this history to be repeated. Published by Elsevier B.V. C1 [Bormann, Bernard T.] USDA, Pacific NW Res Stn, Forest Serv, Corvallis, OR 97331 USA. [Darbyshire, Robyn L.] USDA, Pacific NW Res Stn, Forest Serv, Portland, OR USA. [Homann, Peter S.] Western Washington Univ, Huxley Coll Environm, Dept Environm Sci, Bellingham, WA 98225 USA. [Morrissette, Brett A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Little, Susan N.] USDA, Pacific NW Res Stn, Forest Serv, Portland, OR USA. RP Bormann, BT (reprint author), USDA, Pacific NW Res Stn, Forest Serv, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM bbormann@fs.fed.us; rdarbyshire@fs.fed.us; Peter.Homann@wwu.edu; brett.morrissette@oregonstate.edu; myrtleconn@earthlink.net FU Joint Fire Sciences Program [03-2-3-09, 10-1-10-18]; National Commission for Science on Sustainable Forestry [C4] FX This paper is a contribution of the USDA Forest Service, Pacific Northwest Research Station's Long-Term Ecosystem Productivity Program. Support for pre- and post-treatment, and post-wildfire sampling and analysis came from the Research Station, the US Environmental Protection Agency, Environmental Research Laboratory, Corvallis, Oregon (Interagency Agreement DW 12936179), the Joint Fire Sciences Program (grants 03-2-3-09 and 10-1-10-18), Western Washington University, the National Commission for Science on Sustainable Forestry (grant C4), and the Rogue River Siskiyou National Forest. We acknowledge the hard work of many individuals, including former LTEP-experiment leader Mike Amaranthus; agreement leads from Oregon State University, Kermit Cromack Jr. and Mark Harmon. This work would not have happened without the professional field and laboratory assistants from past years: Tom Bell, Aurore Chauvry, Matt Cowall, Colin Edgar, Laura Fabrey, Nate France, Nick Leahy, Kristina Muscutt, Suzanne Remillard, Vannessa Spini, Chris Stevens, and Kyle Swanson; and from recent years: Amy Barnhart, Dylan Burgess, Nick Daniel, Martyn Davies, Emma Garner, Tim Martin, and Kylie Meyer. We also thank: S. Mark Nay for soil respiration data analysis, Pat Cunningham for statistical guidance, and Paul Anderson, Tom Hanley, Mike Newton, Ross Kiester, Bob Zybach and two anonymous Journal reviewers for their thoughtful comments. NR 58 TC 2 Z9 2 U1 13 U2 74 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 15 PY 2015 VL 340 BP 114 EP 125 DI 10.1016/j.foreco.2014.12.016 PG 12 WC Forestry SC Forestry GA CD2SQ UT WOS:000350929700012 ER PT J AU Rathjens, H Oppelt, N Bosch, DD Arnold, JG Volk, M AF Rathjens, H. Oppelt, N. Bosch, D. D. Arnold, J. G. Volk, M. TI Development of a grid-based version of the SWAT landscape model SO HYDROLOGICAL PROCESSES LA English DT Article DE raster-based simulation; watershed modeling; discretization scheme; SWATgrid; landscape routing ID HYDROLOGICALLY SENSITIVE AREAS; COASTAL-PLAIN; NATURAL CATCHMENTS; SURFACE SATURATION; DRAINAGE DENSITY; CHANNEL; HILLSLOPE; RUNOFF; INDEX; SOIL AB Integrated river basin models should provide a spatially distributed representation of basin hydrology and transport processes to allow for spatially implementing specific management and conservation measures. To accomplish this, the Soil and Water Assessment Tool (SWAT) was modified by integrating a landscape routing model to simulate water flow across discretized routing units. This paper presents a grid-based version of the SWAT landscape model that has been developed to enhance the spatial representation of hydrology and transport processes. The modified model uses a new flow separation index that considers topographic features and soil properties to capture channel and landscape flow processes related to specific landscape positions. The resulting model is spatially fully distributed and includes surface, lateral and groundwater fluxes in each grid cell of the watershed. Furthermore, it more closely represents the spatially heterogeneous distributed flow and transport processes in a watershed. The model was calibrated and validated for the Little River Watershed (LRW) near Tifton, Georgia (USA). Water balance simulations as well as the spatial distribution of surface runoff, subsurface flow and evapotranspiration are examined. Model results indicate that groundwater flow is the dominant landscape process in the LRW. Results are promising, and satisfactory output was obtained with the presented grid-based SWAT landscape model. Nash-Sutcliffe model efficiencies for daily stream flow were 0.59 and 0.63 for calibration and validation periods, and the model reasonably simulates the impact of the landscape position on surface runoff, subsurface flow and evapotranspiration. Additional revision of the model will likely be necessary to adequately represent temporal variations of transport and flow processes in a watershed. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Rathjens, H.; Oppelt, N.] Univ Kiel, Dept Geog, D-24098 Kiel, Germany. [Bosch, D. D.] ARS, USDA, Southeast Watershed Res Lab, Tifton, GA 31793 USA. [Arnold, J. G.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Volk, M.] Helmholtz Ctr Environm Res UFZ Leipzig, Dept Computat Landscape Ecol, D-04318 Leipzig, Germany. RP Rathjens, H (reprint author), Univ Kiel, Dept Geog, Ludewig Meyn Str 14, D-24098 Kiel, Germany. EM rathjens@geographie.uni-kiel.de RI Volk, Martin/F-1172-2010 OI Volk, Martin/0000-0003-0064-8133 NR 60 TC 11 Z9 11 U1 2 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD MAR 15 PY 2015 VL 29 IS 6 BP 900 EP 914 DI 10.1002/hyp.10197 PG 15 WC Water Resources SC Water Resources GA CC7LD UT WOS:000350548100007 ER PT J AU Brown, KR McGuire, KJ Aust, WM Hession, WC Dolloff, CA AF Brown, Kristopher R. McGuire, Kevin J. Aust, W. Michael Hession, W. Cully Dolloff, C. Andrew TI The effect of increasing gravel cover on forest roads for reduced sediment delivery to stream crossings SO HYDROLOGICAL PROCESSES LA English DT Article DE forest roads; stream crossing approaches; rainfall simulation; sediment delivery; BMP cost-effectiveness; non-point-source pollution ID RAINFALL SIMULATION; DYNAMIC ERODIBILITY; EROSION PREDICTION; NORTHERN THAILAND; VIRGINIA PIEDMONT; WATER-QUALITY; UNPAVED ROADS; RUNOFF; GENERATION; VALIDATION AB Direct sediment inputs from forest roads at stream crossings are a major concern for water quality and aquatic habitat. Legacy road-stream crossing approaches, or the section of road leading to the stream, may have poor water and grade control upon reopening, thus increasing the potential for negative impacts to water quality. Rainfall simulation experiments were conducted on the entire running surface area associated with six reopened stream crossing approaches in the south-western Virginia Piedmont physiographic region, USA. Event-based surface run-off and associated total suspended solid (TSS) concentrations were compared among a succession of gravel surfacing treatments that represented increasing intensities of best management practice (BMP) implementation. The three treatments were no gravel (10-19% cover), low gravel (34-60% cover), and high gravel (50-99% cover). Increased field hydraulic conductivity was associated with maximized surface cover and ranged from 7.2 to 41.6, 11.9 to 46.3, and 16.0 to 58.6mmh(-1) respectively for the no gravel, low gravel, and high gravel treatments. Median TSS concentration of surface run-off for the no gravel treatment (2.84gl(-1)) was greater than low gravel (1.10gl(-1)) and high gravel (0.82gl(-1)) by factors of 2.6 and 3.5 respectively. Stream crossing approaches with 90-99% surface cover had TSS concentrations below 1gl(-1). Reducing the length of road segments that drain directly to the stream can reduce the costs associated with gravel surfacing. This research demonstrates that judicious and low-cost BMPs can ameliorate poor water control and soil erosion associated with reopening legacy roads. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Brown, Kristopher R.] Virginia Tech, Dept Forest Resources & Environm Conservat FREC, Blacksburg, VA 24061 USA. [McGuire, Kevin J.] Virginia Tech, Virginia Water Resources Res Ctr, FREC, Blacksburg, VA 24061 USA. [Aust, W. Michael] Virginia Tech, FREC, Blacksburg, VA 24061 USA. [Hession, W. Cully] Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA. [Dolloff, C. Andrew] Virginia Tech, USDA Forest Serv, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. RP Brown, KR (reprint author), Virginia Tech, Dept Forest Resources & Environm Conservat FREC, 210 Cheatham Hall,310 West Campus Dr, Blacksburg, VA 24061 USA. EM krisrb3@vt.edu RI McGuire, Kevin/E-7770-2010 OI McGuire, Kevin/0000-0001-5751-3956 FU Virginia Department of Forestry; Virginia Tech College of Natural Resources and Environment; Virginia Water Resources Research Center; RHFRRC FX Funding and support for this research were provided by the Virginia Department of Forestry, Virginia Tech College of Natural Resources and Environment, Virginia Water Resources Research Center, and RHFRRC. Laura Lehmann provided instruction regarding the operation of the pump and rainfall simulator, which belonged to the Department of Biological Systems Engineering at Virginia Tech. Brian Morris, Clay Sawyers, AJ Lang, and others provided invaluable field help in conducting the rainfall simulation experiments. We thank Pamela Edwards (USDA Forest Service, Northern Research Station) as well as two anonymous reviewers for their critical reviews of previous versions of this manuscript. Ana Maria Ortega Villa and Ian Crandell from the Laboratory for Interdisciplinary Statistical Analysis of Virginia Tech provided statistical consulting for this research. NR 38 TC 4 Z9 4 U1 3 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD MAR 15 PY 2015 VL 29 IS 6 BP 1129 EP 1140 DI 10.1002/hyp.10232 PG 12 WC Water Resources SC Water Resources GA CC7LD UT WOS:000350548100025 ER PT J AU Johnson, JB Gelvin, AB Duvoy, P Schaefer, GL Poole, G Horton, GD AF Johnson, Jerome B. Gelvin, Arthur B. Duvoy, Paul Schaefer, Garry L. Poole, Garry Horton, Glenn D. TI Performance characteristics of a new electronic snow water equivalent sensor in different climates SO HYDROLOGICAL PROCESSES LA English DT Article DE snow water equivalent; electronic sensor; hydrology; field measurements; snow pillow AB The US Army ERDC CRREL and the US Department of Agriculture Natural Resources Conservation Service developed a square electronic snow water equivalent (e-SWE) sensor as an alternative to using fluid-filled snow pillows to measure SWE. The sensors consist of a centre panel to measure SWE and eight outer panels to buffer edge stress concentrations. Seven 3m square e-SWE sensors were installed in five different climate zones. During the 2011-2012 winter, 1.8 and 1.2m square e-SWE sensors were installed and operated in Oregon. With the exception of New York State and Newfoundland, the e-SWE sensors accurately measured SWE, with R-2 values between the sensor and manual SWE measurements of between 0.86 and 0.98. The e-SWE sensor at Hogg Pass, Oregon, accurately measured SWE during the past 8years of operations. In the thin, icy snow of New York during midwinter 2008-2009, the e-SWE sensors overmeasured SWE because of edge stress concentrations associated with strong icy layers and a shallow snow cover. The New York e-SWE sensors' measurement accuracy improved in spring 2009 and further improved during the 2011-2012 winter with operating experience. At Santiam Junction, measured SWE from the 1.8 and 1.2m square e-SWE sensors agreed well with the snow pillow, 3m square e-SWE sensor, and manual SWE measurements until February 2013, when dust and gravel blew onto the testing area resulting in anomalous measurements. (c) 2014 The Authors. Hydrological Processes published by John Wiley & Sons Ltd. C1 [Johnson, Jerome B.; Duvoy, Paul] Univ Alaska Fairbanks, Inst Northern Engn, Fairbanks, AK 99775 USA. [Gelvin, Arthur B.] US Army ERDC Cold Reg Res & Engn Lab, Ft Wainwright, AK 99703 USA. [Schaefer, Garry L.] Nat Resources Conservat Serv, USDA, Portland, OR 97232 USA. [Poole, Garry] Newfoundland & Labrador Hydro, St John, NF A1B 4K7, Canada. [Horton, Glenn D.] New York City Environm Protect, Grahamsville, NY 12740 USA. RP Johnson, JB (reprint author), Univ Alaska Fairbanks, Inst Northern Engn, POB 755910, Fairbanks, AK 99775 USA. EM jerome.b.johnson@alaska.edu FU USDA NRCS; Institute of Northern Engineering, University of Alaska Fairbanks FX This work was supported by the USDA NRCS and the Institute of Northern Engineering, University of Alaska Fairbanks. We gratefully acknowledge the SnowNet project (http://www.ipysnow.net/) for using their preliminary SWE data for Barrow and Imnavait. NR 30 TC 1 Z9 1 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD MAR 15 PY 2015 VL 29 IS 6 BP 1418 EP 1433 DI 10.1002/hyp.10211 PG 16 WC Water Resources SC Water Resources GA CC7LD UT WOS:000350548100047 ER PT J AU Vithanage, M Rajapaksha, AU Ahmad, M Uchimiya, M Dou, XM Alessi, DS Ok, YS AF Vithanage, Meththika Rajapaksha, Anushka Upamali Ahmad, Mahtab Uchimiya, Minori Dou, Xiaomin Alessi, Daniel S. Ok, Yong Sik TI Mechanisms of antimony adsorption onto soybean stover-derived biochar in aqueous solutions SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Black carbon; Surface complexation model; Charcoal; Slow pyrolysis; Surface charge ID SURFACE FUNCTIONALITY; FAST PYROLYSIS; HEAVY-METALS; SOIL; ENVIRONMENT; WATERS; CARBON; RETENTION; CHEMISTRY; SORPTION AB Limited mechanistic knowledge is available on the interaction of biochar with trace elements (Sb and As) that exist predominantly as oxoanions. Soybean stover biochars were produced at 300 degrees C (SBC300) and 700 degrees C (SBC700), and characterized by BET, Boehm titration, FT-IR, NMR and Raman spectroscopy. Bound protons were quantified by potentiometric titration, and two acidic sites were used to model biochar by the surface complexation modeling based on Boehm titration and NMR observations. The zero point of charge was observed at pH 7.20 and 7.75 for SBC300 and SBC700, respectively. Neither antimonate (Sb(V)) nor antimonite (Sb(III)) showed ionic strength dependency (0.1, 0.01 and 0.001 M NaNO3), indicating inner sphere complexation. Greater adsorption of Sb(III) and Sb(V) was observed for SBC300 having higher OH content than SBC700. Sb(III) removal (85%) was greater than Sb(V) removal (68%). Maximum adsorption density for Sb(III) was calculated as 1.88 x 10(-6) mol m(2). The Triple Layer Model (TLM) successfully described surface complexation of Sb onto soybean stover-derived biochar at pH 4-9, and suggested the formation of monodentate mononuclear and binuclear complexes. Spectroscopic investigations by Raman, FT-IR and XPS further confirmed strong chemisorptive binding of Sb to biochar surfaces. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Vithanage, Meththika; Rajapaksha, Anushka Upamali; Ok, Yong Sik] Kangwon Natl Univ, Korea Biochar Res Ctr, Chunchon 200701, South Korea. [Vithanage, Meththika; Rajapaksha, Anushka Upamali] Inst Fundamental Studies, Chem & Environm Syst Modeling Res Grp, Kandy, Sri Lanka. [Ahmad, Mahtab] King Saud Univ, Coll Food & Agr Sci, Dept Soil Sci, Riyadh 11451, Saudi Arabia. [Uchimiya, Minori] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Dou, Xiaomin] Beijing Forestry Univ, Coll Environm Sci & Engn, Beijing 100083, Peoples R China. [Alessi, Daniel S.] Univ Alberta, Dept Earth & Atmospher Sci, Edmonton, AB T6G 2E3, Canada. RP Ok, YS (reprint author), Kangwon Natl Univ, Dept Environm Biol, Chunchon 200701, South Korea. EM meththikavithanage@gmail.com; anushkaupamali@gmail.com; mah_tabee@yahoo.com; Sophie.Uchimiya@ARS.USDA.GOV; alessi@ualberta.ca; soilok@kangwon.ac.kr RI Dou, Xiaomin/G-3899-2012; Ahmad, Mahtab/D-5076-2014; OI Dou, Xiaomin/0000-0003-2583-2244; Ahmad, Mahtab/0000-0003-1853-5161; Rajapaksha, Anushka Upamali/0000-0002-3240-3934; Alessi, Daniel/0000-0002-8360-8251 FU Basic Science Research Foundation through the National Research Foundation (NRF) of Korea; Ministry of Education, Science and Technology [2012R1A1B3001409]; Korea Basic Science Institute; Central Laboratory of the Kangwon National University in Korea FX This research was supported by Basic Science Research Foundation through the National Research Foundation (NRF) of Korea, funded by the Ministry of Education, Science and Technology (2012R1A1B3001409). The instrumental analysis was partly supported by the Korea Basic Science Institute and the Central Laboratory of the Kangwon National University in Korea. NR 40 TC 8 Z9 10 U1 23 U2 106 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD MAR 15 PY 2015 VL 151 BP 443 EP 449 DI 10.1016/j.jenvman.2014.11.005 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CC1FZ UT WOS:000350087900046 PM 25602696 ER PT J AU Aronstein, K Colby, D AF Aronstein, Kate Colby, Deanna TI A multiplex PCR assay for determination of mating type in isolates of the honey bee fungal pathogen, Ascosphaera apis SO JOURNAL OF APICULTURAL RESEARCH LA English DT Article DE fungal pathogen; mating type; honeybee; Ascosphaera apis; PCR C1 [Aronstein, Kate; Colby, Deanna] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA USA. RP Aronstein, K (reprint author), USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA USA. EM Kate.Aronstein@ars.usda.gov FU Agriculture and Food Research Initiative Competitive [2009-85118-05718] FX This work was supported by Agriculture and Food Research Initiative Competitive [grant number 2009-85118-05718]. NR 3 TC 0 Z9 0 U1 2 U2 2 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0021-8839 EI 2078-6913 J9 J APICULT RES JI J. Apic. Res. PD MAR 15 PY 2015 VL 54 IS 2 BP 105 EP 107 DI 10.1080/00218839.2015.1109917 PG 3 WC Entomology SC Entomology GA DF3MD UT WOS:000371247400007 ER PT J AU Danihlik, J Aronstein, K Petrivalsky, M AF Danihlik, Jiri Aronstein, Kate Petrivalsky, Marek TI Antimicrobial peptides: a key component of honey bee innate immunity Physiology, biochemistry, and chemical ecology SO JOURNAL OF APICULTURAL RESEARCH LA English DT Review DE honey bee; antimicrobial peptides; defensins; innate immunity; Varroa destructor; royalisin; Apis mellifera; Nosema; hymenoptaecin; viruses; apidaecins; abaecin; jelleine ID APIS-MELLIFERA; GENE-EXPRESSION; SOCIAL IMMUNITY; NOSEMA-CERANAE; ANTIBACTERIAL RESPONSE; PAENIBACILLUS-LARVAE; HYMENOPTERA APIDAE; HYGIENIC BEHAVIOR; VARROA-DESTRUCTOR; ANTIBIOTICS AB Honey bee immune responses are composed of a complex suite of individual immune mechanisms and special types of behavioral adaptations. The main focus of this paper is innate immunity in the honey bee, and specifically, the role and function of antimicrobial peptides (AMPs). Insect innate immunity constitutes evolutionary conserved defense strategies that provide immediate responses against invading pathogens. It consists of the three levels of resistance: physical barriers as the first line of defense, cell-mediated immunity, and cell-free humoral immunity, a complex network of intracellular signaling pathways leading to activation of a variety of humoral factors. Among those, AMPs are recognized as key components of humoral immunity in many types of organisms. The two basic mechanisms of action are: (1) the generation of leaks into prokaryotic membranes; and (2) either inhibition of bacterial protein translation or folding. Recently, four families of AMPs (i.e., apidaecins, abaecin, hymenoptaecin and defensins) have been described in the honey bee. One of the defensins, defensin1, was originally isolated from royal jelly, and therefore named royalisin. In addition, several bioactive peptides (e.g., apamin and melittin) were identified in bee venom. The expression of bee AMPs is regulated mainly by two intracellular signaling pathways Toll and Imd/JNK. However, the extent of gene expression and peptides synthesis is affected by a number of different biotic and abiotic factors. In this review paper, we have attempted to discuss factors involved in activation of the honey bee AMPs and their role in bee resistance to microbial pathogens and environmental stress such as exposure to pesticides. We also discuss recent knowledge on the molecular regulation of bee AMPs. Although recent advances in genomics have produced a new understanding of bee immunity in general, the exact mechanisms of gene regulation within each of the immune signaling pathways and the complex network of these signaling pathways still await further investigations. C1 [Danihlik, Jiri; Petrivalsky, Marek] Palacky Univ, Fac Sci, Dept Biochem, CR-77147 Olomouc, Czech Republic. [Danihlik, Jiri] Palacky Univ, Fac Sci, Dept Prot Biochem & Prote, Ctr Reg Hana Biotechnol & Agr Res, CR-77147 Olomouc, Czech Republic. [Aronstein, Kate] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA USA. RP Petrivalsky, M (reprint author), Palacky Univ, Fac Sci, Dept Biochem, CR-77147 Olomouc, Czech Republic. EM marek.petrivalsky@upol.cz NR 118 TC 1 Z9 1 U1 23 U2 35 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0021-8839 EI 2078-6913 J9 J APICULT RES JI J. Apic. Res. PD MAR 15 PY 2015 VL 54 IS 2 BP 123 EP 136 DI 10.1080/00218839.2015.1109919 PG 14 WC Entomology SC Entomology GA DF3MD UT WOS:000371247400010 ER PT J AU Liu, Y Qiu, S Li, JL Chen, H Tatsumi, E Yadav, M Yin, LJ AF Liu, Yan Qiu, Shuang Li, Jinlong Chen, Hao Tatsumi, Eizo Yadav, Madhav Yin, Lijun TI Peroxidase-mediated conjugation of corn fiber gum and bovine serum albumin to improve emulsifying properties SO CARBOHYDRATE POLYMERS LA English DT Article DE Corn fiber gum; Peroxidase; Hetero-conjugation; Emulsifying ability ID SUGAR-BEET PECTIN; CATALYZED CROSS-LINKING; IN-WATER EMULSIONS; FERULOYLATED ARABINOXYLANS; HORSERADISH-PEROXIDASE; STABILIZED EMULSIONS; SODIUM CASEINATE; IONIC-STRENGTH; FERULIC ACID; PROTEIN AB The emulsifying properties of corn fiber gum (CFG), a naturally occurring polysaccharide-protein complex, was improved by kinetically controlled formation of hetero-covalent linkages with bovine serum albumin (BSA), using horseradish peroxidase (HRP). The formation of hetero-crosslinked CFG-BSA conjugates was confirmed using ultraviolet-visible and Fourier-transform infrared analyses. The optimum CFG-BSA conjugates were prepared at a CFG:BSA weight ratio of 10:1, and peroxidase:BSA weight ratio of 1:4000. Selected CFG-BSA conjugates were used to prepare oil-in-water emulsions; the emulsifying properties were better than those of emulsions stabilized with only CFG or BSA. Measurements of mean droplet sizes and zeta potentials showed that CFG-BSA-conjugate-stabilized emulsions were less susceptible to environmental stresses, such as pH changes, high K ionic strengths, and freeze-thaw treatments than CFG- or BSA-stabilized emulsions. These conjugates have potential applications as novel emulsifiers in food industry. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Liu, Yan; Qiu, Shuang; Li, Jinlong; Chen, Hao; Yin, Lijun] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China. [Tatsumi, Eizo] Japan Int Res Ctr Agr Sci, Food Sci & Technol Div, Ibaraki 3058686, Japan. [Yadav, Madhav] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Yin, Lijun] Henan Univ Technol, Coll Food Sci & Technol, Zhengzhou 450052, Peoples R China. RP Yin, LJ (reprint author), POB 40,17 Qinghua East Rd, Beijing 100083, Peoples R China. EM ljyin@cau.edu.cn FU National Science Foundation of China [31371719]; National Key-technologies RD Project [2012BAD37B05]; special fund for agro-scientific research in the public interest [201303079] FX This research was carried out with financial support from the National Science Foundation of China (No. 31371719) and National Key-technologies R&D Project (No. 2012BAD37B05) and special fund for agro-scientific research in the public interest (No. 201303079). NR 46 TC 2 Z9 2 U1 6 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD MAR 15 PY 2015 VL 118 BP 70 EP 78 DI 10.1016/j.carbpol.2014.10.059 PG 9 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA AZ5KL UT WOS:000348259900011 PM 25542109 ER PT J AU Wang, ZH Mi, TJ Beier, RC Zhang, HY Sheng, YJ Shi, WM Zhang, SX Shen, JZ AF Wang, Zhanhui Mi, Tiejun Beier, Ross C. Zhang, Huiyan Sheng, Yajie Shi, Weimin Zhang, Suxia Shen, Jianzhong TI Hapten synthesis, monoclonal antibody production and development of a competitive indirect enzyme-linked immunosorbent assay for erythromycin in milk SO FOOD CHEMISTRY LA English DT Article DE Cross-reactivity; Enzyme-linked immunosorbent assay; Erythromycin; Hapten incorporation; Macrolides; Monoclonal antibody ID TANDEM MASS-SPECTROMETRY; MACROLIDE ANTIBIOTICS; GROWTH PROMOTERS; RESIDUES; IMMUNOASSAY; NANOPARTICLES; SULFONAMIDE; EXTRACTION; MUSCLE; SWINE AB Erythromycin is an antibiotic used extensively in veterinary practice worldwide for treatment, prevention and growth promotion. In this work, monoclonal antibodies (Mabs) against erythromycin were produced and used to develop a competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of erythromycin in milk. A novel carboxyphenyl derivative of erythromycin (ERO-CMO) was synthesized and conjugated with bovine serum (BSA) for use as the immunogen or ovalbumin (OVA) as the coating antigen. Four hybridoma cell lines were isolated, which produced Mabs that competed with erythromycin. The 6C1 and 5B2 Mabs had IC50 values for erythromycin of 14.40 and 0.94 mu g L-1, respectively. These Mabs demonstrated high cross-reactivity to the macrolides containing 14-membered rings, but not to oleandomycin. No cross-reactivity was observed for 12 macrolides that contained 15 or 16-membered lactone rings or for 2 pleuromutilins. The ciELISA developed using the 5B2 Mab afforded recovery values that ranged from 76.9% to 85.7% with only a 10-fold sample dilution prior to analysis. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Wang, Zhanhui; Mi, Tiejun; Zhang, Huiyan; Sheng, Yajie; Shi, Weimin; Zhang, Suxia; Shen, Jianzhong] China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China. [Beier, Ross C.] USDA ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Shen, JZ (reprint author), China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China. EM sjz@cau.edu.cn OI Wang, Zhanhui/0000-0002-0167-9559 FU Trans-Century Training Programme Foundation for Talents by the Ministry of Education [NCET-12-0529]; State Key Program of the National Natural Science of China [30830082] FX This work is supported by grants from the Trans-Century Training Programme Foundation for Talents by the Ministry of Education (NCET-12-0529) and the State Key Program of the National Natural Science of China (No. 30830082). NR 27 TC 6 Z9 7 U1 4 U2 129 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2015 VL 171 BP 98 EP 107 DI 10.1016/j.foodchem.2014.08.104 PG 10 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AS0EZ UT WOS:000343952300015 PM 25308648 ER PT J AU Du, XF Song, M Baldwin, E Rouseff, R AF Du, Xiaofen Song, Mei Baldwin, Elizabeth Rouseff, Russell TI Identification of sulphur volatiles and GC-olfactometry aroma profiling in two fresh tomato cultivars SO FOOD CHEMISTRY LA English DT Article DE Tomato sulphur; Tomato GC-O; FL 47; Tasti-Lee ID MARKET TOMATOES; PASTE VOLATILES; FLAVOR; QUALITY; PERCEPTION; COMPONENTS; FRUITS; DESCRIPTORS; ATTRIBUTES; PRODUCTS AB Ten sulphur volatiles were observed in two Florida tomato cultivars ('Tasti-Lee' and 'FL 47') harvested at three maturity stages (breaker, turning, and pink) using gas chromatography with a pulsed flame photometric detector (GC-PFPD). Eight PFPD peaks were identified using retention values from authentic sulphur standards and GC-MS characteristic masses. Seven were quantified using an internal standard combined with external calibration curves. Dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide 2-propylthiazole and 2-s-butylthiazole were newly identified in fresh tomatoes. Principal component analysis of sulphur volatiles indicated that there were appreciable maturity stage differences clustered in separate quadrants. GC-olfactometry (GC-O) identified 50 aroma-active compounds in 'Tasti-Lee', with 10 reported as odorants in fresh tomatoes for the first time. Four sulphur volatiles exhibited aroma activity, including two of the newly-reported fresh tomato sulphur volatiles, 2-s-butylthiazole and dimethyl sulphide. GC-O aroma profiling indicated that the most intense aroma category was earthy-musty, followed by fruity-floral, green-grassy, sweet-candy and sweaty-stale-sulphurous. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Du, Xiaofen; Song, Mei; Rouseff, Russell] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Baldwin, Elizabeth] USDA ARS, Hort Res Lab, Ft Pierce, FL 33881 USA. RP Du, XF (reprint author), Univ Florida, Citrus Res & Educ Ctr, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. EM xiaofendu09@gmail.com FU USDA Specialty Crops Research Initiative entitled "Increasing Consumption of Fresh Fruits & Vegetables by Enhancing Their Quality and Safety" FX This study was supported in part by a USDA Specialty Crops Research Initiative entitled "Increasing Consumption of Fresh Fruits & Vegetables by Enhancing Their Quality and Safety" as a joint research project with the University of California, Davis and the University of Florida, USA. Authors thank J.W. Scott (Gulf Coast Research and Education Centre, University of Florida, Florida) for supplying raw tomato samples. NR 40 TC 7 Z9 8 U1 5 U2 134 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAR 15 PY 2015 VL 171 BP 306 EP 314 DI 10.1016/j.foodchem.2014.09.013 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AS0EZ UT WOS:000343952300041 PM 25308674 ER PT J AU Basler, C Forshey, TM Machesky, K Erdman, CM Gomez, TM Brinson, DL Nguyen, TA Behravesh, CB Bosch, S AF Basler, Colin Forshey, Tony M. Machesky, Kimberly Erdman, C. Matthew Gomez, Thomas M. Brinson, Denise L. Thai-An Nguyen Behravesh, Casey Barton Bosch, Stacey TI Multistate Outbreak of Human Salmonella Infections Linked to Live Poultry from a Mail-Order Hatchery in Ohio - February-October 2014 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID MARCH-SEPTEMBER C1 [Basler, Colin] CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA. [Basler, Colin; Thai-An Nguyen; Behravesh, Casey Barton; Bosch, Stacey] CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Forshey, Tony M.] Ohio Dept Agr, Reynoldsburg, OH USA. [Machesky, Kimberly] Ohio Dept Hlth, Columbus, OH 43266 USA. [Erdman, C. Matthew; Gomez, Thomas M.; Brinson, Denise L.] USDA, Washington, DC USA. RP Basler, C (reprint author), CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA. EM cbasler@cdc.gov NR 4 TC 1 Z9 1 U1 0 U2 2 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD MAR 13 PY 2015 VL 64 IS 9 BP 258 EP 258 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CE2HF UT WOS:000351635300005 PM 25763879 ER PT J AU Nambeesan, SU Mandel, JR Bowers, JE Marek, LF Ebert, D Corbi, J Rieseberg, LH Knapp, SJ Burke, JM AF Nambeesan, Savithri U. Mandel, Jennifer R. Bowers, John E. Marek, Laura F. Ebert, Daniel Corbi, Jonathan Rieseberg, Loren H. Knapp, Steven J. Burke, John M. TI Association mapping in sunflower (Helianthus annuus L.) reveals independent control of apical vs. basal branching SO BMC PLANT BIOLOGY LA English DT Article DE Apical dominance; Association mapping; Branching; Helianthus annuus; Linkage disequilibrium; Plant architecture; Sunflower ID GENOME-WIDE ASSOCIATION; AXILLARY BUD OUTGROWTH; POPULATION-STRUCTURE; MERISTEM INITIATION; GENETIC ASSOCIATION; COMPLEX TRAITS; ARABIDOPSIS; DOMINANCE; GROWTH; ARCHITECTURE AB Background: Shoot branching is an important determinant of plant architecture and influences various aspects of growth and development. Selection on branching has also played an important role in the domestication of crop plants, including sunflower (Helianthus annuus L.). Here, we describe an investigation of the genetic basis of variation in branching in sunflower via association mapping in a diverse collection of cultivated sunflower lines. Results: Detailed phenotypic analyses revealed extensive variation in the extent and type of branching within the focal population. After correcting for population structure and kinship, association analyses were performed using a genome-wide collection of SNPs to identify genomic regions that influence a variety of branching-related traits. This work resulted in the identification of multiple previously unidentified genomic regions that contribute to variation in branching. Genomic regions that were associated with apical and mid-apical branching were generally distinct from those associated with basal and mid-basal branching. Homologs of known branching genes from other study systems (i.e., Arabidopsis, rice, pea, and petunia) were also identified from the draft assembly of the sunflower genome and their map positions were compared to those of associations identified herein. Numerous candidate branching genes were found to map in close proximity to significant branching associations. Conclusions: In sunflower, variation in branching is genetically complex and overall branching patterns (i.e., apical vs. basal) were found to be influenced by distinct genomic regions. Moreover, numerous candidate branching genes mapped in close proximity to significant branching associations. Although the sunflower genome exhibits localized islands of elevated linkage disequilibrium (LD), these non-random associations are known to decay rapidly elsewhere. The subset of candidate genes that co-localized with significant associations in regions of low LD represents the most promising target for future functional analyses. C1 [Nambeesan, Savithri U.; Mandel, Jennifer R.; Bowers, John E.; Corbi, Jonathan; Burke, John M.] Univ Georgia, Dept Plant Biol Miller Plant Sci, Athens, GA 30602 USA. [Marek, Laura F.] Iowa State Univ, USDA ARS, North Cent Reg Plant Intro Stn, Ames, IA 50014 USA. [Ebert, Daniel; Rieseberg, Loren H.] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. [Knapp, Steven J.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Burke, JM (reprint author), Univ Georgia, Dept Plant Biol Miller Plant Sci, Athens, GA 30602 USA. EM jmburke@uga.edu RI Bowers, John/B-9245-2009; Burke, John/A-3502-2013; OI Burke, John/0000-0002-1412-5539; Rieseberg, Loren/0000-0002-2712-2417 FU USDA National Institute of Food and Agriculture [2008-35300-19263]; NSF Plant Genome Research Program [DBI-0820451]; USDA/DOE Plant Feedstocks Genomics Joint Program [ER 64664]; Genome BC; Genome Canada FX We thank Eric Elsner, Jenny Leverett, Irvine Larsen, Tanya MacInnes, Anh Nguyen, and many undergraduate workers for their assistance in the field. This research was supported by USDA National Institute of Food and Agriculture (2008-35300-19263), the NSF Plant Genome Research Program (DBI-0820451), the USDA/DOE Plant Feedstocks Genomics Joint Program (ER 64664), Genome BC, and Genome Canada (http://www.genomecanada.ca). NR 79 TC 1 Z9 1 U1 2 U2 27 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAR 11 PY 2015 VL 15 AR 84 DI 10.1186/s12870-015-0458-9 PG 12 WC Plant Sciences SC Plant Sciences GA CG5FY UT WOS:000353317700001 PM 25887675 ER PT J AU Norman, KN Clawson, ML Strockbine, NA Mandrell, RE Johnson, R Ziebell, K Zhao, SH Fratamico, PM Stones, R Allard, MW Bono, JL AF Norman, Keri N. Clawson, Michael L. Strockbine, Nancy A. Mandrell, Robert E. Johnson, Roger Ziebell, Kim Zhao, Shaohua Fratamico, Pina M. Stones, Robert Allard, Marc W. Bono, James L. TI Comparison of whole genome sequences from human and non-human Escherichia coil 026 strains SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE Escherichia coli; O26; Shiga toxins; polymorphisms; phylogenetic ID COLI SEROGROUPS O26; ANTIMICROBIAL RESISTANCE; PHYLOGENETIC ANALYSIS; UNITED-STATES; MINCED BEEF; CATTLE; O111; INFECTIONS; OUTBREAK; O157 AB Shiga toxin-producing Escherichia coil (STEC) O26 is the second leading E co/i serogroup responsible for human illness outbreaks behind E coli O157:H7. Recent outbreaks have been linked to emerging pathogenic O26:H11 strains harboring stx2 only. Cattle have been recognized as an important reservoir of O26 strains harboring stx(1); however the reservoir of these emerging stx(2) strains is unknown. The objective of this study was to identify nucleotide polymorphisms in human and cattle-derived strains in order to compare differences in polymorphism derived genotypes and virulence gene profiles between the two host species. Whole genome sequencing was performed on 182 epidemiologically unrelated O26 strains, including 109 human-derived strains and 73 non-human-derived strains. A panel of 289 O26 strains (241 STEC and 48 non-STEC) was subsequently genotyped using a set of 283 polymorphisms identified by whole genome sequencing, resulting in 64 unique genotypes. Phylogenetic analyses identified seven clusters within the O26 strains. The seven clusters did not distinguish between isolates originating from humans or cattle; however, clusters did correspond with particular virulence gene profiles. Human and non-human-derived strains harboring stx(1) clustered separately from strains harboring stx2, strains harboring eae, and non-STEC strains. Strains harboring stx(2)were more closely related to non-STEC strains and strains harboring eae than to strains harboring stx(1). The finding of human and cattle-derived strains with the same polymorphism derived genotypes and similar virulence gene profiles, provides evidence that similar strains are found in cattle and humans and transmission between the two species may occur. C1 [Norman, Keri N.; Clawson, Michael L.; Bono, James L.] ARS, US Meat Anim Res Ctr, USDA, Clay Ctr, NE 68933 USA. [Strockbine, Nancy A.] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA. [Mandrell, Robert E.] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. [Johnson, Roger; Ziebell, Kim] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Guelph, ON, Canada. [Zhao, Shaohua] US FDA, Div Anim & Food Microbiol, Ctr Vet Med, Laurel, MD USA. [Fratamico, Pina M.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA. [Stones, Robert] Food & Environm Res Agcy, York, N Yorkshire, England. [Allard, Marc W.] US FDA, Div Microbiol, Ctr Food Safety & Appl Nutr, Off Regulatory Sci, College Pk, MD USA. RP Bono, JL (reprint author), ARS, US Meat Anim Res Ctr, USDA, State Spur 18D,POB 166, Clay Ctr, NE 68933 USA. EM jim.bono@ars.usda.gov FU USDA CSREES NRI FX We would like to thank Sandy Fryda-Bradley, Linda Flathman, and Chris Zimmerman for outstanding technical support. The .sff files for both human and non-human DNA pools have been deposited into the short read archives under BioProject accession PRJNA274335. Some strains used were collected as part of a USDA CSREES NRI grant awarded to Dr. James Keen. The use of product and company names is necessary to accurately report the methods and results; however, the USDA neither guarantees nor warrants the standard of the products, and the use of the names by the USDA implies no approval of the products to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 44 TC 2 Z9 2 U1 1 U2 4 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD MAR 11 PY 2015 VL 5 AR 21 DI 10.3389/fcimb.2015.00021 PG 10 WC Immunology; Microbiology SC Immunology; Microbiology GA CF3MW UT WOS:000352454000001 PM 25815275 ER PT J AU Holtman, KM Offeman, RD Franqui-Villanueva, D Bayati, AK Orts, WJ AF Holtman, Kevin M. Offeman, Richard D. Franqui-Villanueva, Diana Bayati, Andre K. Orts, William J. TI Countercurrent Extraction of Soluble Sugars from Almond Hulls and Assessment of the Bioenergy Potential SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE almond hulls; carbohydrates; fermentable sugars; countercurrent extraction; fermentation; ethanol; biogas ID CHEMICAL-COMPOSITION; PECTIN AB Almond hulls contain considerable proportions (37% by dry weight) of water-soluble, fermentable sugars (sucrose, glucose, and fructose), which can be extracted for industrial purposes. The maximum optimal solids loading was determined to be 20% for sugar extraction, and the addition of 0.5% (w/v) pectinase aided in maintaining a sufficient free water volume for sugar recovery. A laboratory countercurrent extraction experiment utilizing a 1 h steep followed by three extraction (wash) stages produced a high-concentration (131 g/L fermentable sugar) syrup. Overall, sugar recovery efficiency was 88%. The inner stage washing efficiencies were compatible with solution equilibrium calculations, indicating that efficiency was high. The concentrated sugar syrup was fermented to ethanol at high efficiency (86% conversion), and ethanol concentrations in the broth were 7.4% (v/v). Thin stillage contained 233 g SCOD/L, which was converted to biomethane at an efficiency of 90% with a biomethane potential of 297 mL/g SCODdestroyed. Overall, results suggested that a minima of 49 gal (185 L) ethanol and 75 m3 methane/t hulls (dry whole hull basis) are achievable. C1 [Holtman, Kevin M.; Offeman, Richard D.; Franqui-Villanueva, Diana; Bayati, Andre K.; Orts, William J.] ARS, PWA, WRRC, BCE,USDA, Albany, CA 94710 USA. RP Holtman, KM (reprint author), ARS, PWA, WRRC, BCE,USDA, 800 Buchanan St, Albany, CA 94710 USA. EM kevin.holtman@ars.usda.gov FU Almond Hullers and Processors Association (AHPA) under Trust Fund Cooperative Agreement [58-5325-2-140] FX We thank the Almond Hullers and Processors Association (AHPA) for financial support under Trust Fund Cooperative Agreement 58-5325-2-140. NR 26 TC 1 Z9 1 U1 7 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 11 PY 2015 VL 63 IS 9 BP 2490 EP 2498 DI 10.1021/jf5048332 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CD6GE UT WOS:000351186800016 PM 25693995 ER PT J AU Tizioto, PC Taylor, JF Decker, JE Gromboni, CF Mudadu, MA Schnabel, RD Coutinho, LL Mourao, GB Oliveira, PSN Souza, MM Reecy, JM Nassu, RT Bressani, FA Tholon, P Sonstegard, TS Alencar, MM Tullio, RR Nogueira, ARA Regitano, LCA AF Tizioto, Polyana C. Taylor, Jeremy F. Decker, Jared E. Gromboni, Caio F. Mudadu, Mauricio A. Schnabel, Robert D. Coutinho, Luiz L. Mourao, Gerson B. Oliveira, Priscila S. N. Souza, Marcela M. Reecy, James M. Nassu, Renata T. Bressani, Flavia A. Tholon, Patricia Sonstegard, Tad S. Alencar, Mauricio M. Tullio, Rymer R. Nogueira, Ana R. A. Regitano, Luciana C. A. TI Detection of quantitative trait loci for mineral content of Nelore longissimus dorsi muscle SO GENETICS SELECTION EVOLUTION LA English DT Article ID GENOME-WIDE ASSOCIATION; SINGLE NUCLEOTIDE POLYMORPHISM; MEAT QUALITY TRAITS; BEEF-CATTLE; BOVINE LIVER; BOS-TAURUS; TENDERNESS; GENE; CALPASTATIN; IRON AB Background: Beef cattle require dietary minerals for optimal health, production and reproduction. Concentrations of minerals in tissues are at least partly genetically determined. Mapping genomic regions that affect the mineral content of bovine longissimus dorsi muscle can contribute to the identification of genes that control mineral balance, transportation, absorption and excretion and that could be associated to metabolic disorders. Methods: We applied a genome-wide association strategy and genotyped 373 Nelore steers from 34 half-sib families with the Illumina BovineHD BeadChip. Genome-wide association analysis was performed for mineral content of longissimus dorsi muscle using a Bayesian approach implemented in the GenSel software. Results: Muscle mineral content in Bos indicus cattle was moderately heritable, with estimates ranging from 0.29 to 0.36. Our results suggest that variation in mineral content is influenced by numerous small-effect QTL (quantitative trait loci) but a large-effect QTL that explained 6.5% of the additive genetic variance in iron content was detected at 72 Mb on bovine chromosome 12. Most of the candidate genes present in the QTL regions for mineral content were involved in signal transduction, signaling pathways via integral (also called intrinsic) membrane proteins, transcription regulation or metal ion binding. Conclusions: This study identified QTL and candidate genes that affect the mineral content of skeletal muscle. Our findings provide the first step towards understanding the molecular basis of mineral balance in bovine muscle and can also serve as a basis for the study of mineral balance in other organisms. C1 [Tizioto, Polyana C.; Oliveira, Priscila S. N.; Souza, Marcela M.] Univ Fed Sao Carlos, Dept Genet & Evolut, BR-13560 Sao Carlos, SP, Brazil. [Tizioto, Polyana C.; Taylor, Jeremy F.; Decker, Jared E.; Schnabel, Robert D.] Univ Missouri, Div Anim Sci, Columbia, MO USA. [Gromboni, Caio F.] Bahia Sci & Technol, Fed Inst Educ, Valenca, BA, Brazil. [Mudadu, Mauricio A.; Nassu, Renata T.; Bressani, Flavia A.; Tholon, Patricia; Alencar, Mauricio M.; Tullio, Rymer R.; Nogueira, Ana R. A.; Regitano, Luciana C. A.] Embrapa Southeast Livestock, Sao Carlos, SP, Brazil. [Coutinho, Luiz L.; Mourao, Gerson B.] Univ Sao Paulo, Dept Anim Sci, ESALQ, Piracicaba, SP, Brazil. [Sonstegard, Tad S.] USDA ARS, Beltsville, MD USA. [Reecy, James M.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. RP Regitano, LCA (reprint author), Embrapa Southeast Livestock, Sao Carlos, SP, Brazil. EM luciana.regitano@embrapa.br RI Regitano, Luciana/C-7002-2012; de Alvarenga Mudadu, Mauricio/B-6524-2014; Nassu, Renata/B-7627-2012; Coutinho, Luiz/C-8455-2012; Mourao, Gerson/D-2064-2012; Nogueira, Ana Rita/D-9319-2012 OI Regitano, Luciana/0000-0001-9242-8351; de Alvarenga Mudadu, Mauricio/0000-0002-2767-2507; Mourao, Gerson/0000-0002-0990-4108; Nogueira, Ana Rita/0000-0003-3648-2956 FU National Council of Technological and Scientific Development (CNPq); Paulo Research Foundation (FAPESP) [2010/06515-4, 2012/01502-7] FX We thank Dr Gustavo Gasparin and Dr Wilson Malago Jr for technical assistance; Dr Michele Lopes do Nascimento, Dr Andrea Souza and MSc Amalia Saturnino for monitoring the feedlots; Dr Michel EB Yamaguishi and BSc Fabio D Vieira for technical assistance with database maintenance, Dr Dorian Garrick for data analysis support, the University of Missouri for accepting the first author as a visiting scholar, and the National Council of Technological and Scientific Development (CNPq) for providing fellowships to GB Mourao, LL Coutinho, MM Alencar, ARA Nogueira and LCA Regitano, and the scholarship provided to CF Gromboni. We also thank Sao Paulo Research Foundation (FAPESP) for providing scholarships #2010/06515-4 and #2012/01502-7 to PC Tizioto. NR 31 TC 4 Z9 4 U1 0 U2 16 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD MAR 11 PY 2015 VL 47 AR 15 DI 10.1186/s12711-014-0083-3 PG 9 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CD3AZ UT WOS:000350951400001 PM 25880074 ER PT J AU Sabur, MA Goldberg, S Gale, A Kabengi, N Al-Abadleh, HA AF Sabur, Md Abdus Goldberg, Sabine Gale, Adrian Kabengi, Nadine Al-Abadleh, Hind A. TI Temperature-Dependent Infrared and Calorimetric Studies on Arsenicals Adsorption from Solution to Hematite Nanoparticles SO LANGMUIR LA English DT Article ID ISOTHERMAL TITRATION CALORIMETRY; FUNCTIONAL THEORY CALCULATIONS; SURFACE COMPLEXATION; SPECTROSCOPIC CHARACTERIZATION; ARSENATE COORDINATION; PHOSPHORUS SORPTION; PHOSPHATE SORPTION; AQUEOUS-SOLUTIONS; FERRIC HYDROXIDE; ATR-FTIR AB To address the lack of systematic and surface sensitive studies on the adsorption energetics of arsenic compounds on metal (oxyhydr)oxides, we conducted temperature-dependent ATR-FTIR studies for the adsorption of arsenate, monomethylarsonic acid, and dimethylarsinic acid on hematite nanoparticles at pH 7. Spectra were collected as a function of concentration and temperature in the range 5-50 degrees C (278-323 K). Adsorption isotherms were constructed from spectral features assigned to surface arsenic. Values of Keq, adsorption enthalpy, and entropy were extracted from fitting the Langmuir model to the data and from custom-built triple-layer surface complexation models derived from our understanding of the adsorption mechanism of each arsenical. These spectroscopic and modeling results were complemented with flow-through calorimetric measurements of molar heats of adsorption. Endothermic adsorption processes were predicted from the application of mathematical models with a net positive change in adsorption entropy. However, experimentally measured heats of adsorption were exothermic for all three arsenicals studied herein, with arsenate releasing 1.6-1.9 times more heat than methylated arsenicals. These results highlight the role of hydration thermodynamics on the adsorption of arsenicals, and are consistent with the spectral interpretation of type of surface complexes each arsenical form in that arsenate is mostly dominated by bidentate, MMA by a mixture of mono- and bidentate, and DMA by mostly outer sphere. C1 [Sabur, Md Abdus; Al-Abadleh, Hind A.] Wilfrid Laurier Univ, Dept Chem & Biochem, Waterloo, ON N2L 3C5, Canada. [Goldberg, Sabine] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Gale, Adrian; Kabengi, Nadine] Georgia State Univ, Dept Geosci, Atlanta, GA 30303 USA. RP Al-Abadleh, HA (reprint author), Wilfrid Laurier Univ, Dept Chem & Biochem, Waterloo, ON N2L 3C5, Canada. EM halabadleh@wlu.ca FU Laurier; NSERC; Ontario's Ministry of Research and Innovation; U.S. Department of Energy (DOE) Office of Science Early Career Research Program [211267] FX H.A.A. acknowledges partial funding from Laurier, NSERC, and Early Researcher Award from Ontario's Ministry of Research and Innovation. N.K. acknowledges the support of the U.S. Department of Energy (DOE) Office of Science Early Career Research Program Grant No. 211267. NR 47 TC 9 Z9 9 U1 7 U2 50 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0743-7463 J9 LANGMUIR JI Langmuir PD MAR 10 PY 2015 VL 31 IS 9 BP 2749 EP 2760 DI 10.1021/la504581p PG 12 WC Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary SC Chemistry; Materials Science GA CD2OI UT WOS:000350918500017 PM 25695733 ER PT J AU Greenlee, MHW Smith, JD Platt, EM Juarez, JR Timms, LL Greenlee, JJ AF Greenlee, M. Heather West Smith, Jodi D. Platt, Ekundayo M. Juarez, Jessica R. Timms, Leo L. Greenlee, Justin J. TI Changes in Retinal Function and Morphology Are Early Clinical Signs of Disease in Cattle with Bovine Spongiform Encephalopathy SO PLOS ONE LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; CHRONIC WASTING DISEASE; OPTICAL COHERENCE TOMOGRAPHY; TRANSMISSIBLE MINK ENCEPHALOPATHY; ELECTRORETINOGRAM B-WAVE; PROTEIN GENE PRNP; PRION PROTEIN; PARKINSON DISEASE; CEREBROSPINAL-FLUID; BIPOLAR CELLS AB Bovine spongiform encephalopathy (BSE) belongs to a group of fatal, transmissible protein misfolding diseases known as transmissible spongiform encephalopathies (TSEs). All TSEs are caused by accumulation of misfolded prion protein (PrPSc) throughout the central nervous system (CNS), which results in neuronal loss and ultimately death. Like other protein misfolding diseases including Parkinson's disease and Alzheimer's disease, TSEs are generally not diagnosed until the onset of disease after the appearance of unequivocal clinical signs. As such, identification of the earliest clinical signs of disease may facilitate diagnosis. The retina is the most accessible part of the central nervous system, and retinal pathology in TSE affected animals has been previously reported. Here we describe antemortem changes in retinal function and morphology that are detectable in BSE inoculated animals several months (up to 11 months) prior to the appearance of any other signs of clinical disease. We also demonstrate that differences in the severity of these clinical signs reflect the amount of PrPSc accumulation in the retina and the resulting inflammatory response of the tissue. These results are the earliest reported clinical signs associated with TSE infection and provide a basis for understanding the pathology and evaluating therapeutic interventions. C1 [Greenlee, M. Heather West] Iowa State Univ, Dept Biomed Sci, Ames, IA 50010 USA. [Greenlee, M. Heather West; Platt, Ekundayo M.] Iowa State Univ, Interdept Toxicol Program, Ames, IA 50010 USA. [Smith, Jodi D.; Greenlee, Justin J.] Natl Anim Dis Ctr, Virus & Prion Res Unit, Ames, IA 50010 USA. [Platt, Ekundayo M.] Iowa State Univ, Dept Genet & Cell Biol, Ames, IA 50010 USA. [Juarez, Jessica R.; Timms, Leo L.] Iowa State Univ, Dept Anim Sci, Ames, IA 50010 USA. RP Greenlee, MHW (reprint author), Iowa State Univ, Dept Biomed Sci, Ames, IA 50010 USA. EM mheather@iastate.edu FU Agricultural Research Service; Agricultural Research Service, US Department of Agriculture [58-3625-5-114] FX This work was funded by the Agricultural Research Service 'Transmission, differentiation, and pathobiology of transmissible spongiform encephalopathies'; and the Specific Cooperative Agreement #58-3625-5-114 with the Agricultural Research Service, US Department of Agriculture. The funders had no role in study design, data collection and analysis, or preparation of the manuscript. NR 55 TC 5 Z9 5 U1 0 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 10 PY 2015 VL 10 IS 3 AR UNSP e0119431 DI 10.1371/journal.pone.0119431 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD7MX UT WOS:000351275700045 PM 25756286 ER PT J AU Manavalan, LP Prince, SJ Musket, TA Chaky, J Deshmukh, R Vuong, TD Song, L Cregan, PB Nelson, JC Shannon, JG Specht, JE Nguyen, HT AF Manavalan, Lakshmi P. Prince, Silvas J. Musket, Theresa A. Chaky, Julian Deshmukh, Rupesh Vuong, Tri D. Song, Li Cregan, Perry B. Nelson, James C. Shannon, J. Grover Specht, James E. Nguyen, Henry T. TI Identification of Novel QTL Governing Root Architectural Traits in an Interspecific Soybean Population SO PLOS ONE LA English DT Article ID CYST-NEMATODE RESISTANCE; MAX L. MERR.; RECOMBINANT INBRED LINES; GLYCINE-SOJA SIEB.; DROUGHT TOLERANCE; TRANSCRIPTION FACTOR; NITROGEN-FIXATION; WATER RELATIONS; YIELD; WILD AB Cultivated soybean (Glycine max L.) cv. Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits. In this study, phenotypic variability for root traits among 251 BC2F5 backcross inbred lines (BILs) developed from the cross Dunbar/PI 326582A were identified. The root systems of the parents and BILs were evaluated in controlled environmental conditions using a cone system at seedling stage. The G. max parent Dunbar contributed phenotypically favorable alleles at a major quantitative trait locus on chromosome 8 (Satt315-I locus) that governed root traits (tap root length and lateral root number) and shoot length. This QTL accounted for >10% of the phenotypic variation of both tap root and shoot length. This QTL region was found to control various shoot-and root-related traits across soybean genetic backgrounds. Within the confidence interval of this region, eleven transcription factors (TFs) were identified. Based on RNA sequencing and Affymetrix expression data, key TFs including MYB, AP2-EREBP and bZIP TFs were identified in this QTL interval with high expression in roots and nodules. The backcross inbred lines with different parental allelic combination showed different expression pattern for six transcription factors selected based on their expression pattern in root tissues. It appears that the marker interval Satt315-I locus on chromosome 8 contain an essential QTL contributing to early root and shoot growth in soybean. C1 [Manavalan, Lakshmi P.; Prince, Silvas J.; Musket, Theresa A.; Deshmukh, Rupesh; Vuong, Tri D.; Song, Li; Shannon, J. Grover; Nguyen, Henry T.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Chaky, Julian; Specht, James E.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD USA. [Nelson, James C.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Nguyen, HT (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM nguyenhenry@missouri.edu FU United Soybean Board; Missouri Soybean Merchandising Council FX This research was supported by the United Soybean Board, http://unitedsoybean.org/, and Missouri Soybean Merchandising Council, http://mosoy.org/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 81 TC 8 Z9 9 U1 3 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 10 PY 2015 VL 10 IS 3 AR UNSP e0120490 DI 10.1371/journal.pone.0120490 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CD7MX UT WOS:000351275700067 PM 25756528 ER PT J AU Kovalev, V Petkov, A Wold, C Urbanski, S Hao, WM AF Kovalev, V. Petkov, A. Wold, C. Urbanski, S. Hao, W. M. TI Determination of the smoke-plume heights and their dynamics with ground-based scanning lidar SO APPLIED OPTICS LA English DT Article ID FIRE EMISSIONS; CLIMATE AB Lidar-data processing techniques are analyzed, which allow determining smoke-plume heights and their dynamics and can be helpful for the improvement of smoke dispersion and air quality models. The data processing algorithms considered in the paper are based on the analysis of two alternative characteristics related to the smoke dispersion process: the regularized intercept function, extracted directly from the recorded lidar signal, and the square-range corrected backscatter signal, obtained after determining and subtracting the constant offset in the recorded signal. The analysis is performed using experimental data of the scanning lidar obtained in the area of prescribed fires. (C) 2015 Optical Society of America C1 [Kovalev, V.; Petkov, A.; Wold, C.; Urbanski, S.; Hao, W. M.] Forest Serv, USDA, Fire Sci Lab, Missoula, MT 59808 USA. RP Kovalev, V (reprint author), Forest Serv, USDA, Fire Sci Lab, 5775 Hwy 10 West, Missoula, MT 59808 USA. EM vladimirakovalev@fs.fed.us NR 14 TC 0 Z9 0 U1 1 U2 4 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 1559-128X EI 2155-3165 J9 APPL OPTICS JI Appl. Optics PD MAR 10 PY 2015 VL 54 IS 8 BP 2011 EP 2017 DI 10.1364/AO.54.002011 PG 7 WC Optics SC Optics GA CC9AE UT WOS:000350658900024 PM 25968377 ER PT J AU Reichel, MP Moore, DP Hemphill, A Ortega-Mora, LM Dubey, JP Ellis, JT AF Reichel, Michael P. Moore, Dadin P. Hemphill, Andrew Ortega-Mora, Luis M. Dubey, J. P. Ellis, John T. TI A live vaccine against Neospora caninum abortions in cattle SO VACCINE LA English DT Editorial Material ID DEFINITIVE HOSTS; TOXOPLASMOSIS C1 [Reichel, Michael P.] Univ Adelaide, Sch Anim & Vet Sci, Roseworthy, SA 5371, Australia. [Moore, Dadin P.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Hemphill, Andrew] Univ Bern, Inst Parasitol, Vetsuisse Fac, CH-3012 Bern, Switzerland. [Ortega-Mora, Luis M.] Univ Complutense Madrid, Fac Vet Sci, Dept Anim Hlth, SALUVET, E-28040 Madrid, Spain. [Dubey, J. P.] ARS, USDA, APDL BARC, Beltsville, MD 20705 USA. [Ellis, John T.] Univ Technol Sydney, Sch Med & Mol Biosci, Sydney, NSW 2007, Australia. RP Reichel, MP (reprint author), Univ Adelaide, Sch Anim & Vet Sci, Roseworthy Campus, Roseworthy, SA 5371, Australia. EM michael.reichel@adelaide.edu.au RI Reichel, Michael/B-8685-2013; Ortega-Mora, Luis-Miguel/F-1470-2016; Ellis, John/L-6988-2016 OI Reichel, Michael/0000-0002-9792-8336; Ortega-Mora, Luis-Miguel/0000-0002-4986-6783; Ellis, John/0000-0001-7328-4831 NR 23 TC 6 Z9 6 U1 5 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD MAR 10 PY 2015 VL 33 IS 11 BP 1299 EP 1301 DI 10.1016/j.vaccine.2015.01.064 PG 3 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA CD2RB UT WOS:000350925600001 PM 25659274 ER PT J AU Bertran, K Moresco, K Swayne, DE AF Bertran, Kateri Moresco, Kira Swayne, David E. TI Impact of vaccination on infection with Vietnam H5N1 high pathogenicity avian influenza virus in hens and the eggs they lay SO VACCINE LA English DT Article DE Chicken; Layers; Egg production; High pathogenicity avian influenza; H5N1; Vaccine ID OIL-EMULSION VACCINES; HONG-KONG; CLINICAL SIGNS; CHICKEN EGGS; A VIRUSES; PROTECTION; EFFICACY; DUCKS; EVOLUTION; OUTBREAK AB High pathogenicity avian influenza virus (HPAIV) infections in chickens negatively impact egg production and cause egg contamination. Previously, vaccination maintained egg production and reduced egg contamination when challenged with a North American H5N2 HPAIV. However, Asian H5N1 HPAIV infection has some characteristics of increased pathogenicity compared to other H5 HPAIV such as more rapid drop and complete cessation in egg production. Sham (vaccinated at 25 and 28 weeks of age), inactivated H5N1 Once (1X-H5-Vax; vaccinated at 28 weeks of age only) and inactivated H5N1 Twice (2X-H5-Vax; vaccinated at 25 and 28 weeks of age) vaccinated adult White Leghorn hens were challenged intranasally at 31 weeks of age with 6.1 log(10) mean embryo infectious doses (EID50) of clade 2.3.2.1a H5N1 HPAIV (AlchickeniVietnam/NCVD-675/2011) which was homologous to the inactivated vaccine. Sham-vaccinated layers experienced 100% mortality within 3 days post-challenge; laid soft and thin-shelled eggs; had recovery of virus from oral swabs and in 53% of the eggs from eggshell surface (35%), yolk (24%), and albumin (41%); and had very high titers of virus (average 7.91 log(10) EID50/g) in all segments of the oviduct and ovary. By comparison, 1X- and 2X-H5-Vax challenged hens survived infection, laid similar number of eggs pre- and post-challenge, all eggs had normal egg shell quality, and had significantly fewer contaminated eggs with reduced virus quantity. The 2X-H5-Vax hens had significantly higher HI titers by the day of challenge (304 GMT) and at termination (512 GMT) than 1X-H5-Vax hens (45 GMT and 128 GMT). The current study demonstrated that AIV infections caused by clade 2.3.2.1a H5N1 variants can be effectively controlled by either double or single homologous vaccination. Published by Elsevier Ltd. C1 [Bertran, Kateri; Moresco, Kira; Swayne, David E.] USDA ARS, Exot & Emerging Avian Viral Dis Res Unit, Athens, GA 30605 USA. RP Swayne, DE (reprint author), USDA ARS, Exot & Emerging Avian Viral Dis Res Unit, 934 Coll Stn Rd, Athens, GA 30605 USA. EM David.Swayne@ars.usda.gov FU Agricultural Research Service, USDA, Current Research Information Service [6612-32000-063-00D] FX This work was funded by Agricultural Research Service, USDA, Current Research Information Service project 6612-32000-063-00D. The authors gratefully acknowledge James Doster, Roger Brock, and Mariana Sa e Silva for their excellent technical assistance. Thanks to Dr. Nguyen Tung, National Centre for Veterinary Diagnostics, and Drs. Todd David and Ruben Donis, Centers for Disease Control and Prevention, for providing the vaccine and challenge virus. NR 48 TC 1 Z9 1 U1 1 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD MAR 10 PY 2015 VL 33 IS 11 BP 1324 EP 1330 DI 10.1016/j.vaccine.2015.01.055 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA CD2RB UT WOS:000350925600005 PM 25657093 ER PT J AU Chiou, BS Valenzuela-Medina, D Wechsler, M Bilbao-Sainz, C Klamczynski, AK Williams, TG Wood, DF Glenn, GM Orts, WJ AF Chiou, Bor-Sen Valenzuela-Medina, Diana Wechsler, Mark Bilbao-Sainz, Cristina Klamczynski, Artur K. Williams, Tina G. Wood, Delilah F. Glenn, Greg M. Orts, William J. TI Torrefied Biomass-Polypropylene Composites SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE cellulose and other wood products; composites; properties and characterization; thermoplastics ID PALM WOOD FLOUR; MECHANICAL-PROPERTIES; GREEN COMPOSITES; MORPHOLOGICAL PROPERTIES; POLYMER COMPOSITES; MALEIC-ANHYDRIDE; COUPLING AGENT; LIGNIN; FIBER; TORREFACTION AB Torrefied almond shells and wood chips were incorporated into polypropylene as fillers to produce torrefied biomass-polymer composites. The composites were prepared by extrusion and injection molding. Response surface methodology was used to examine the effects of filler concentration, filler size, and lignin factor (relative lignin to cellulose concentration) on the material properties of the composites. The heat distortion temperatures, thermal properties, and tensile properties of the composites were characterized by thermomechanical analysis, differential scanning calorimetry, and tensile tests, respectively. The torrefied biomass composites had heat distortion temperatures of 8-24 degrees C higher than that of neat polypropylene. This was due to the torrefied biomass restricting mobility of polypropylene chains, leading to higher temperatures for deformation. The incorporation of torrefied biomass generally resulted in an increase in glass transition temperature, but did not affect melting temperature. Also, the composites had lower tensile strength and elongation at break values than those of neat polypropylene, indicating weak adhesion between torrefied biomass and polypropylene. However, scanning electron microscopy results did indicate some adhesion between torrefied biomass and polypropylene. (C) 2014 Wiley Periodicals, Inc. C1 [Chiou, Bor-Sen; Valenzuela-Medina, Diana; Bilbao-Sainz, Cristina; Klamczynski, Artur K.; Williams, Tina G.; Wood, Delilah F.; Glenn, Greg M.; Orts, William J.] USDA, Albany, CA 94710 USA. [Wechsler, Mark] Renewable Fuel Technol, San Mateo, CA 94403 USA. RP Chiou, BS (reprint author), USDA, Albany, CA 94710 USA. EM bor-sen.chiou@ars.usda.gov NR 37 TC 0 Z9 0 U1 2 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD MAR 10 PY 2015 VL 132 IS 10 AR 41582 DI 10.1002/app.41582 PG 8 WC Polymer Science SC Polymer Science GA AW6DH UT WOS:000346359100011 ER PT J AU Bassil, NV Davis, TM Zhang, HL Ficklin, S Mittmann, M Webster, T Mahoney, L Wood, D Alperin, ES Rosyara, UR Putten, HKV Monfort, A Sargent, DJ Amaya, I Denoyes, B Bianco, L van Dijk, T Pirani, A Iezzoni, A Main, D Peace, C Yang, YL Whitaker, V Verma, S Bellon, L Brew, F Herrera, R de Weg, EV AF Bassil, Nahla V. Davis, Thomas M. Zhang, Hailong Ficklin, Stephen Mittmann, Mike Webster, Teresa Mahoney, Lise Wood, David Alperin, Elisabeth S. Rosyara, Umesh R. Putten, Herma Koehorst-vanc Monfort, Amparo Sargent, Daniel J. Amaya, Iraida Denoyes, Beatrice Bianco, Luca van Dijk, Thijs Pirani, Ali Iezzoni, Amy Main, Dorrie Peace, Cameron Yang, Yilong Whitaker, Vance Verma, Sujeet Bellon, Laurent Brew, Fiona Herrera, Raul de Weg, Eric van TI Development and preliminary evaluation of a 90 K Axiom (R) SNP array for the allo-octoploid cultivated strawberry Fragaria x ananassa SO BMC GENOMICS LA English DT Article DE Fragaria; Genotyping array; Plant breeding; Polyploidy; Strawberry; Single nucleotide polymorphism; Reduced ploidy ID SINGLE-NUCLEOTIDE POLYMORPHISMS; LINKAGE MAP; GENOME SEQUENCE; APPLE; WHEAT; GENE; IDENTIFICATION; REARRANGEMENTS; DUPLICATION; IMPROVEMENT AB Background: A high-throughput genotyping platform is needed to enable marker-assisted breeding in the allo-octoploid cultivated strawberry Fragaria x ananassa. Short-read sequences from one diploid and 19 octoploid accessions were aligned to the diploid Fragaria vesca 'Hawaii 4' reference genome to identify single nucleotide polymorphisms (SNPs) and indels for incorporation into a 90 K Affymetrix (R) Axiom (R) array. We report the development and preliminary evaluation of this array. Results: About 36 million sequence variants were identified in a 19 member, octoploid germplasm panel. Strategies and filtering pipelines were developed to identify and incorporate markers of several types: di-allelic SNPs (66.6%), multi-allelic SNPs (1.8%), indels (10.1%), and ploidy-reducing "haploSNPs" (11.7%). The remaining SNPs included those discovered in the diploid progenitor F. iinumae (3.9%), and speculative "codon-based" SNPs (5.9%). In genotyping 306 octoploid accessions, SNPs were assigned to six classes with Affymetrix's "SNPolisher" R package. The highest quality classes, PolyHigh Resolution (PHR), No Minor Homozygote (NMH), and Off-Target Variant (OTV) comprised 25%, 38%, and 1% of array markers, respectively. These markers were suitable for genetic studies as demonstrated in the full-sib family 'Holiday' x 'Korona' with the generation of a genetic linkage map consisting of 6,594 PHR SNPs evenly distributed across 28 chromosomes with an average density of approximately one marker per 0.5 cM, thus exceeding our goal of one marker per cM. Conclusions: The Affymetrix IStraw90 Axiom array is the first high-throughput genotyping platform for cultivated strawberry and is commercially available to the worldwide scientific community. The array's high success rate is likely driven by the presence of naturally occurring variation in ploidy level within the nominally octoploid genome, and by effectiveness of the employed array design and ploidy-reducing strategies. This array enables genetic analyses including generation of high-density linkage maps, identification of quantitative trait loci for economically important traits, and genome-wide association studies, thus providing a basis for marker-assisted breeding in this high value crop. C1 [Bassil, Nahla V.; Alperin, Elisabeth S.] USDA ARS, NCGR, Corvallis, OR 97331 USA. [Davis, Thomas M.; Zhang, Hailong; Mahoney, Lise; Wood, David; Yang, Yilong] Univ New Hampshire, Durham, NH 03824 USA. [Ficklin, Stephen; Main, Dorrie; Peace, Cameron] Washington State Univ, Pullman, WA 99164 USA. [Mittmann, Mike; Webster, Teresa; Pirani, Ali] Affymetrix, Santa Clara, CA USA. [Rosyara, Umesh R.; Iezzoni, Amy] Michigan State Univ, E Lansing, MI 48824 USA. [Putten, Herma Koehorst-vanc; van Dijk, Thijs; de Weg, Eric van] Wageningen UR Plant Breeding, Wageningen, Netherlands. [Monfort, Amparo] CSIC IRTA UAB UB, IRTA Ctr Res Agr Genom, Barcelona, Spain. [Sargent, Daniel J.; Bianco, Luca] Fdn Edmund Mach, Res & Innovat Ctr, I-38010 San Michele All Adige, TN, Italy. [Amaya, Iraida] IFAPA Ctr Churriana, Malaga, Spain. [Denoyes, Beatrice] INRA, UMR BFP, Bordeaux, France. [Whitaker, Vance; Verma, Sujeet] Univ Florida, GCREC, Wimauma, FL USA. [Bellon, Laurent; Brew, Fiona] Affymetrix UK Ltd, Wooburn Green, High Wycombe, England. [Herrera, Raul] Univ Talca, Inst Ciencias Biol, Talca, Chile. RP Bassil, NV (reprint author), USDA ARS, NCGR, Corvallis, OR 97331 USA. EM nahla.bassil@ars.usda.gov RI Monfort, Amparo/K-1356-2015 OI Monfort, Amparo/0000-0001-7106-7745 FU USDA's National Institute of Food and Agriculture - Specialty Crop Research Initiative project, "RosBREED: Enabling marker-assisted breeding in Rosaceae" [2009-51181-05808]; New Hampshire Agricultural Experiment Station [NH00433, NH00588]; USDA-CSREES National Research Initiative (NRI) Plant Genome Grant [2008-35300-04411]; Technological Top Institute Green Genetics [1C004RP]; Spanish Ministry of Science [AGL2010-21414]; USDA-ARS CRIS [5358-21000-044-00D] FX This is Scientific Contribution Number 2565 from the New Hampshire Agricultural Experiment Station. This study was partially funded by the USDA's National Institute of Food and Agriculture - Specialty Crop Research Initiative project, "RosBREED: Enabling marker-assisted breeding in Rosaceae" (2009-51181-05808). Partial funding was also provided by the New Hampshire Agricultural Experiment Station (Projects NH00433 and NH00588 - to TMD), and from USDA-CSREES National Research Initiative (NRI) Plant Genome Grant 2008-35300-04411 (to TMD). Technological Top Institute Green Genetics, project number 1C004RP, is acknowledged for financial support. Financial support was also provided by AGL2010-21414 from the Spanish Ministry of Science (to AM); from the USDA-ARS CRIS number 5358-21000-044-00D (to NVB). Fresh Forward Breeding B.V, The Netherlands provided the Holiday x Korona mapping population, and Yolanda Noordijk isolated DNA from this population. Alan Chambers, University of Florida extracted DNA from 'Sweet Charlie' and 'Winter Dawn' for library preparation. We thank strawberry breeders Jim Hancock (Michigan State University) and Chad Finn (USDA/ARS) for providing breeding selections and populations for analyses, and the USDA-ARS-NCGR staff for their numerous contributions of genetic resources and assistance. We also acknowledge the invaluable help of Oregon State University's Bioinformatics and Core facility staff. We thank Janet Slovin, David Chagne, and Melanie Shields for reviewing the manuscript prior to submission. We also thank an anonymous reviewer whose extensive and detailed comments and suggestions prompted revisions that strengthened the manuscript. NR 66 TC 8 Z9 9 U1 6 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 7 PY 2015 VL 16 DI 10.1186/s12864-015-1310-1 PG 30 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CE5SD UT WOS:000351895100001 ER PT J AU Addison, A Powell, JA Bentz, BJ Six, DL AF Addison, Audrey Powell, James A. Bentz, Barbara J. Six, Diana L. TI Integrating models to investigate critical phenological overlaps in complex ecological interactions: The mountain pine beetle-fungus symbiosis SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article DE Phenology; Dendroctonus ponderosae; Grosmannia clavigera; Ophiostoma montium ID DENDROCTONUS-PONDEROSAE COLEOPTERA; BLUE-STAIN FUNGI; CLIMATE-CHANGE; RANGE EXPANSION; BARK BEETLES; HOST; TEMPERATURE; SCOLYTIDAE; REPRODUCTION; MUTUALISM AB The fates of individual species are often tied to synchronization of phenology, however, few methods have been developed for integrating phenological models involving linked species. In this paper, we focus on mountain pine beetle (MPB, Dendroctonus ponderosae) and its two obligate mutualistic fungi, Grosmannia clavigera and Ophiostoma montium. Growth rates of all three partners are driven by temperature, and their idiosyncratic responses affect interactions at important life stage junctures. One critical phase for MPB-fungus symbiosis occurs just before dispersal of teneral (new) adult beetles, when fungi are acquired and transported in specialized structures (mycangia). Before dispersal, fungi must capture sufficient spatial resources within the tree to ensure contact with teneral adults and get packed into mycangia. Mycangial packing occurs at an unknown time during teneral feeding. We adapt thermal models predicting fungal growth and beetle development to predict overlap between the competing fungi and MPB teneral adult feeding windows and emergence. We consider a spectrum of mycangial packing strategies and describe them in terms of explicit functions with unknown parameters. Rates of growth are fixed by laboratory data, the unknown parameters describing various packing strategies, as well as the degree to which mycangial growth is slowed in woody tissues as compared to agar, are determined by maximum likelihood and two years of field observations. At the field location used, the most likely fungus acquisition strategy for MPB was packing mycangia just prior to emergence. Estimated model parameters suggested large differences in the relative growth rates of the two fungi in trees at the study site, with the most likely model estimating that G. clavigera grew approximately twenty-five times faster than O. montium under the bark, which is completely unexpected in comparison with observed fungal growth on agar. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Addison, Audrey; Powell, James A.] Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. [Bentz, Barbara J.] USDA, Forest Serv, Rocky Mt Res Stn, Logan, UT 84321 USA. [Six, Diana L.] Univ Montana, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. RP Addison, A (reprint author), Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. EM audrey.smith@aggiemail.usu.edu; jim.powell@usu.edu; bbentz@fs.fed.us; diana.six@cfc.umt.edu FU USDA Forest Service; Western Wildland Threat Assessment Center; National Science Foundation [DEB 0918756] FX We would like to thank Jim Vandygriff, Matt Hansen, Joel Martin and Greta Schen-Langenheim for field support and mycangial dissections. We would also like to thank Melissa Moore for fungal identification. Funding was provided by the USDA Forest Service, Western Wildland Threat Assessment Center and a National Science Foundation Grant, DEB 0918756. NR 30 TC 2 Z9 2 U1 2 U2 42 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 EI 1095-8541 J9 J THEOR BIOL JI J. Theor. Biol. PD MAR 7 PY 2015 VL 368 BP 55 EP 66 DI 10.1016/j.jtbi.2014.12.011 PG 12 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA CC1BS UT WOS:000350076800005 PM 25556687 ER PT J AU Ran, Q Li, W Du, Q Yang, CH AF Ran, Qiong Li, Wei Du, Qian Yang, Chenghai TI Hyperspectral image classification for mapping agricultural tillage practices SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE conservation tillage; hyperspectral data; feature extraction; spatial-spectral classification; agricultural remote sensing ID CROP RESIDUE COVER; MORPHOLOGICAL PROFILES; DISCRIMINANT-ANALYSIS AB An efficient classification framework for mapping agricultural tillage practice using hyperspectral remote sensing imagery is proposed, which has the potential to be implemented practically to provide rapid, accurate, and objective surveying data for precision agricultural management and appraisal from large-scale remote sensing images. It includes a local region filter [i.e., Gaussian low-pass filter (GLF)] to extract spatial-spectral features, a dimensionality reduction process [i.e., local fisher's discriminate analysis (LFDA)], and the traditional k-nearest neighbor (KNN) classifier, and is denoted as GLF-LFDA-KNN. Compared to our previously used local average filter and adaptive weighted filter, the GLF also considers spatial features in a small neighborhood, but it emphasizes the central pixel itself and is data-independent; therefore, it can achieve the balance between classification accuracy and computational complexity. The KNN classifier has a lower computational complexity compared to the traditional support vector machine (SVM). After classification separability is enhanced by the GLF and LFDA, the less powerful KNN can outperform SVM and the overall computational cost remains lower. The proposed framework can also outperform the SVM with composite kernel (SVM-CK) that uses spatial-spectral features. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) C1 [Ran, Qiong; Li, Wei] Beijing Univ Chem Technol, Coll Informat Sci & Technol, Beijing 100029, Peoples R China. [Du, Qian] Mississippi State Univ, Dept Elect & Comp Engn, Starkville, MS 39762 USA. [Yang, Chenghai] USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Ran, Q (reprint author), Beijing Univ Chem Technol, Coll Informat Sci & Technol, Beijing 100029, Peoples R China. EM ranqiong@mail.buct.edu.cn FU Natural Science Foundation of China [61302164]; Research Fund for Basic Research in Central Universities [YS1404] FX This work is supported by the Natural Science Foundation of China (No. 61302164) and the Research Fund for Basic Research in Central Universities (No. YS1404). NR 29 TC 2 Z9 2 U1 3 U2 17 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD MAR 6 PY 2015 VL 9 AR 097298 DI 10.1117/1.JRS.9.097298 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CF8SX UT WOS:000352833300001 ER PT J AU Hamrick AF Hamrick TI Body Mass Index: Accounting for Full Time Sedentary Occupation and 24-Hr Self-Reported Time Use (vol 9, e109051, 2014) SO PLOS ONE LA English DT Correction C1 USDA, Econ Res Serv, Washington, DC 20024 USA. RP Hamrick (reprint author), USDA, Econ Res Serv, Washington, DC 20024 USA. NR 1 TC 0 Z9 0 U1 0 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 6 PY 2015 VL 10 IS 3 AR e0118117 DI 10.1371/journal.pone.0118117 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC9KQ UT WOS:000350689400012 ER PT J AU Yu, Q Plotto, A Baldwin, EA Bai, J Huang, M Yu, Y Dhaliwal, HS Gmitter, FG AF Yu, Qibin Plotto, Anne Baldwin, Elizabeth A. Bai, Jinhe Huang, Ming Yu, Yuan Dhaliwal, Harvinder S. Gmitter, Frederick G., Jr. TI Proteomic and metabolomic analyses provide insight into production of volatile and non-volatile flavor components in mandarin hybrid fruit SO BMC PLANT BIOLOGY LA English DT Article DE Apocarotenoid volatiles; Carotenoids; Sesquiterpene synthase; Citrus; Gene expression ID SINENSIS L. OSBECK; TOMATO FLAVOR; FUNCTIONAL-CHARACTERIZATION; TANGERINE HYBRIDS; CITRUS; AROMA; BIOSYNTHESIS; IDENTIFICATION; TRANSCRIPTOME; QUALITY AB Background: Although many of the volatile constituents of flavor and aroma in citrus have been identified, the knowledge of molecular mechanisms and regulation of volatile production are very limited. Our aim was to understand mechanisms of flavor volatile production and regulation in mandarin fruit. Result: Fruits of two mandarin hybrids, Temple and Murcott with contrasting volatile and non-volatile profiles, were collected at three developmental stages. A combination of methods, including the isobaric tags for relative and absolute quantification (iTRAQ), quantitative real-time polymerase chain reaction, gas chromatography, and high-performance liquid chromatography, was used to identify proteins, measure gene expression levels, volatiles, sugars, organic acids and carotenoids. Two thirds of differentially expressed proteins were identified in the pathways of glycolysis, citric acid cycle, amino acid, sugar and starch metabolism. An enzyme encoding valencene synthase gene (Cstps1) was more abundant in Temple than in Murcott. Valencene accounted for 9.4% of total volatile content in Temple, whereas no valencene was detected in Murcott fruit. Murcott expression of Cstps1 is severely reduced. Conclusion: We showed that the diversion of valencene and other sesquiterpenes into the terpenoid pathway together with high production of apocarotenoid volatiles might have resulted in the lower concentration of carotenoids in Temple fruit. C1 [Yu, Qibin; Huang, Ming; Yu, Yuan; Gmitter, Frederick G., Jr.] Univ Florida, Inst Food & Agr Sci, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Plotto, Anne; Baldwin, Elizabeth A.; Bai, Jinhe] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. [Dhaliwal, Harvinder S.] Punjab Agr Univ, Coll Agr, Ludhiana 141004, Punjab, India. RP Gmitter, FG (reprint author), Univ Florida, Inst Food & Agr Sci, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. EM fgmitter@ufl.edu FU New Varieties Development and Management Corporation (NVDMC); Citrus Research and Development Foundation Inc. (CRDF) FX The authors thank Mrs. Misty Holt for collecting fruit samples. This work was partly supported by grants from the New Varieties Development and Management Corporation (NVDMC), and the Citrus Research and Development Foundation Inc. (CRDF), on behalf of the Florida citrus industry. NR 48 TC 4 Z9 4 U1 9 U2 48 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAR 6 PY 2015 VL 15 AR 76 DI 10.1186/s12870-015-0466-9 PG 16 WC Plant Sciences SC Plant Sciences GA CD1SP UT WOS:000350854900001 PM 25848837 ER PT J AU Brullo, C Brullo, S Gaskin, JF Del Galdo, GG Hrusa, GF Salmeri, C AF Brullo, Cristian Brullo, Salvatore Gaskin, John F. Del Galdo, Gianpietro Giusso Hrusa, G. Frederic Salmeri, Cristina TI A new species of Kali (Salsoloideae, Chenopodiaceae) from Sicily, supported by molecular analysis SO PHYTOTAXA LA English DT Article DE Chenopodiaceae; haplotype; ITS; Kali; karyology; new species; phylogeny; Sicily; taxonomy ID SALSOLA-TRAGUS; S.L. CHENOPODIACEAE; C-4 PHOTOSYNTHESIS; EVOLUTION; CALIFORNIA; PHYLOGENY; AUSTRALIS; ORIGIN AB Nomenclatural and taxonomical considerations on Kali, a controversial genus recently segregated from the polyphyletic Salsola s. lat. (Chenopodiaceae), are provided. The Kali group includes annual plants with leaves ending in a spine and lacking hypodermis, having also a cortex alternate to longitudinal chlorenchymatous striae. The species belonging to this genus mainly have a paleotemperate distribution (Europe, Asia and North Africa), occurring as aliens in North America, Australia and South Africa. A new species collected on Mt. Etna (Sicily), and closely related to K. australe, is described and illustrated as K. basalticum Its morphological and molecular features, karyology (2n=54), ecology, distribution, phylogeny and conservation status are examined. In addition, a list of the currently known species of Kali is provided, with some new combinations: Kali nepalense (Grubov) comb. nov., Kali pellucidum (Litvinov) comb. nov., Kali sinkiangense (A.J. Li) comb. nov., Kali gobicolum (Iljin) comb. nov., and Kali ryanii (G.F. Hrusa & Gaskin) comb. nov. C1 [Brullo, Cristian; Brullo, Salvatore; Del Galdo, Gianpietro Giusso] Univ Catania, Dipartimento Sci Biol Geol & Ambientali, I-95125 Catania, Italy. [Gaskin, John F.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Hrusa, G. Frederic] Calif Dept Food & Agr, Plant Pest Diagnost Branch, Sacramento, CA 95832 USA. [Salmeri, Cristina] Univ Palermo, Dipartimento Biol Ambientale & Biodiversita, I-90123 Palermo, Italy. RP Brullo, S (reprint author), Univ Catania, Dipartimento Sci Biol Geol & Ambientali, I-95125 Catania, Italy. EM salvo.brullo@gmail.com; fhrusa@cdfa.ca.gov; cristinasalmeri@gmail.com NR 113 TC 2 Z9 2 U1 3 U2 8 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD MAR 5 PY 2015 VL 201 IS 4 BP 256 EP 277 DI 10.11646/phytotaxa.201.4.2 PG 22 WC Plant Sciences SC Plant Sciences GA CF5DA UT WOS:000352574400002 ER PT J AU Huber, SC Weber, APM AF Huber, Steven Carl Weber, Andreas P. M. CA Frontiers Plant Sci Editorial Off TI Mechanosensitive channel candidate MCA2 is involved in touch-induced root responses in Arabidopsis (Retraction of vol 5, 421, 2014) SO FRONTIERS IN PLANT SCIENCE LA English DT Correction DE mechanosensitive channel; Arabidopsis; root; skewing; waving; calcium; touch response; mechanical stress C1 [Huber, Steven Carl] USDA ARS, Urbana, IL 61801 USA. [Weber, Andreas P. M.] Univ Dusseldorf, Dusseldorf, Germany. RP Huber, SC (reprint author), USDA ARS, Urbana, IL 61801 USA. NR 3 TC 0 Z9 0 U1 5 U2 17 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 5 PY 2015 VL 6 AR 153 DI 10.3389/fpls.2015.00153 PG 2 WC Plant Sciences SC Plant Sciences GA CD3HK UT WOS:000350968800001 ER PT J AU Clawson, ML Redden, R Schuller, G Heaton, MP Workman, A Chitko-McKown, CG Smith, TP Leymaster, KA AF Clawson, Michael L. Redden, Reid Schuller, Gennie Heaton, Michael P. Workman, Aspen Chitko-McKown, Carol G. Smith, Timothy Pl Leymaster, Kreg A. TI Genetic subgroup of small ruminant lentiviruses that infects sheep homozygous for TMEM154 frameshift deletion mutation A4(Delta)53 SO VETERINARY RESEARCH LA English DT Article ID ARTHRITIS-ENCEPHALITIS-VIRUS; PROGRESSIVE PNEUMONIA VIRUS; LINKED-IMMUNOSORBENT-ASSAY; MAEDI-VISNA LENTIVIRUS; PHYLOGENETIC ANALYSIS; CAPRINE; GOATS; TOOL AB Small ruminant lentivirus (SRLV) infections of sheep are influenced by genetics on both the host and pathogen sides. Genetic variation in the ovine transmembrane 154 (TMEM154) gene associates with infection susceptibility, and distinct SRLV genetic subgroups infect sheep in association with their TMEM154 diplotypes. In this study, a novel SRLV subgroup was identified that naturally infected sheep with various TMEM154 diplotypes, including those homozygous for a rare frameshift mutation (A4 delta53), which is predicted to abolish TMEM154 protein function. Thus, these SRLVs may infect sheep that lack functional TMEM154, and may not be restricted by TMEM154 diplotypes in establishing infections. C1 [Clawson, Michael L.; Schuller, Gennie; Heaton, Michael P.; Workman, Aspen; Chitko-McKown, Carol G.; Smith, Timothy Pl; Leymaster, Kreg A.] ARS, USDA, USMARC, Clay Ctr, NE 68933 USA. [Redden, Reid] N Dakota State Univ, Dept Anim Sci, Fargo, ND 58108 USA. RP Clawson, ML (reprint author), ARS, USDA, USMARC, State Spur 18D, Clay Ctr, NE 68933 USA. EM mike.clawson@ars.usda.gov OI Clawson, Michael/0000-0002-3355-5390 FU Agricultural Research Service; North Central Region-Sustainable Agricultural Research and Education program FX This work was supported by the Agricultural Research Service and by funding from the North Central Region-Sustainable Agricultural Research and Education program. We thank Jan Watts for secretarial support. The use of product and company names is necessary to accurately report the methods and results; however, the United States Department of Agriculture (USDA) neither guarantees nor warrants the standard of the products, and the use of names by the USDA implies no approval of the product to the exclusion of others that may also be suitable. The USDA is an equal opportunity provider and employer. NR 28 TC 0 Z9 0 U1 1 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD MAR 5 PY 2015 VL 46 AR 22 DI 10.1186/s13567-015-0162-7 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CC5CS UT WOS:000350376300001 PM 25756342 ER PT J AU Peterson, B Nelson, KJ Seielstad, C Stoker, J Jolly, WM Parsons, R AF Peterson, Birgit Nelson, Kurtis J. Seielstad, Carl Stoker, Jason Jolly, W. Matt Parsons, Russell TI Automated integration of lidar into the LANDFIRE product suite SO REMOTE SENSING LETTERS LA English DT Article ID VEGETATION; HEIGHT; VALIDATION; FIRE AB Accurate information about three-dimensional canopy structure and wildland fuel across the landscape is necessary for fire behaviour modelling system predictions. Remotely sensed data are invaluable for assessing these canopy characteristics over large areas; lidar data, in particular, are uniquely suited for quantifying three-dimensional canopy structure. Although lidar data are increasingly available, they have rarely been applied to wildland fuels mapping efforts, mostly due to two issues. First, the Landscape Fire and Resource Planning Tools (LANDFIRE) program, which has become the default source of large-scale fire behaviour modelling inputs for the US, does not currently incorporate lidar data into the vegetation and fuel mapping process because spatially continuous lidar data are not available at the national scale. Second, while lidar data are available for many land management units across the US, these data are underutilized for fire behaviour applications. This is partly due to a lack of local personnel trained to process and analyse lidar data. This investigation addresses these issues by developing the Creating Hybrid Structure from LANDFIRE/lidar Combinations (CHISLIC) tool. CHISLIC allows individuals to automatically generate a suite of vegetation structure and wildland fuel parameters from lidar data and infuse them into existing LANDFIRE data sets. CHISLIC will become available for wider distribution to the public through a partnership with the U.S. Forest Service's Wildland Fire Assessment System (WFAS) and may be incorporated into the Wildland Fire Decision Support System (WFDSS) with additional design and testing. WFAS and WFDSS are the primary systems used to support tactical and strategic wildland fire management decisions. C1 [Peterson, Birgit] US Geol Survey, Earth Resources Observat & Sci EROS Ctr, ASRC Fed InuTeq, Sioux Falls, SD 57198 USA. [Nelson, Kurtis J.; Stoker, Jason] USGS EROS Ctr, Sioux Falls, SD USA. [Seielstad, Carl] Univ Montana, Natl Ctr Landscape Fire Anal, Missoula, MT 59812 USA. [Jolly, W. Matt; Parsons, Russell] US Forest Serv, USDA, Fire Sci Lab, Missoula, MT USA. RP Peterson, B (reprint author), US Geol Survey, Earth Resources Observat & Sci EROS Ctr, ASRC Fed InuTeq, Sioux Falls, SD 57198 USA. EM bpeterson@usgs.gov OI Stoker, Jason/0000-0003-2455-0931 FU National Aeronautics and Space Administration [NNH12AU731]; USDA Forest Service; USGS [G13PC00028] FX This work was supported in part by the National Aeronautics and Space Administration under Grant # NNH12AU731. The work of Dr Jolly and Dr Parsons was supported by the USDA Forest Service. Dr Peterson's work was performed under USGS contract number G13PC00028. NR 26 TC 1 Z9 1 U1 0 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 2150-704X EI 2150-7058 J9 REMOTE SENS LETT JI Remote Sens. Lett. PD MAR 4 PY 2015 VL 6 IS 3 BP 247 EP 256 DI 10.1080/2150704X.2015.1029086 PG 10 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA CJ0JD UT WOS:000355160700009 ER PT J AU Wang, CY Fuentes-Hernandez, C Liu, JC Dindar, A Choi, S Youngblood, JP Moon, RJ Kippelen, B AF Wang, Cheng-Yin Fuentes-Hernandez, Canek Liu, Jen-Chieh Dindar, Amir Choi, Sangmoo Youngblood, Jeffrey P. Moon, Robert J. Kippelen, Bernard TI Stable Low-Voltage Operation Top-Gate Organic Field-Effect Transistors on Cellulose Nanocrystal Substrates SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE organic field-effect transistor; top-gate geometry; cellulose; low-voltage; ambient stability ID SOLAR-CELLS; PAPER ELECTRONICS; TRANSPARENT; FILM AB We report on the performance and the characterization of top-gate organic field-effect transistors (OFETs), comprising a bilayer gate dielectric of CYTOP/Al2O3 and a solution-processed semiconductor layer made of a blend of TIPS-pentacene:PTAA, fabricated on recyclable cellulose nanocrystal-glycerol (CNC/glycerol) substrates. These OFETs exhibit low operating voltage, low threshold voltage, an average field-effect mobility of 0.11 cm(2)/(V s), and good shelf and operational stability in ambient conditions. To improve the operational stability in ambient a passivation layer of Al2O3 is grown by atomic layer deposition (ALD) directly onto the CNC/glycerol substrates. This layer protects the organic semiconductor layer from moisture and other chemicals that can either permeate through or diffuse out of the substrate. C1 [Wang, Cheng-Yin; Fuentes-Hernandez, Canek; Dindar, Amir; Choi, Sangmoo; Kippelen, Bernard] Georgia Inst Technol, Sch Elect & Comp Engn, COPE, Atlanta, GA 30332 USA. [Liu, Jen-Chieh; Youngblood, Jeffrey P.; Moon, Robert J.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Kippelen, B (reprint author), Georgia Inst Technol, Sch Elect & Comp Engn, COPE, Atlanta, GA 30332 USA. EM kippelen@gatech.edu RI Fuentes Hernandez, Canek/G-3974-2011 FU USDA-Forest Service [11-JV-11111129-118] FX This research was funded in part USDA-Forest Service (Grant No. 11-JV-11111129-118). We thank Dr. Alan Rudie from the USDA Forest Product Laboratory (FRL), Madison, WI, for providing the CNC materials. NR 30 TC 9 Z9 9 U1 6 U2 65 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD MAR 4 PY 2015 VL 7 IS 8 BP 4804 EP 4808 DI 10.1021/am508723a PG 5 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA CG1AL UT WOS:000353005300045 PM 25651811 ER PT J AU Sohail, MU Hume, ME Byrd, JA Nisbet, DJ Shabbir, MZ Ijaz, A Rehman, H AF Sohail, Muhammad U. Hume, Michael E. Byrd, James A. Nisbet, David J. Shabbir, Muhammad Z. Ijaz, Ahmad Rehman, Habib TI Molecular analysis of the caecal and tracheal microbiome of heat-stressed broilers supplemented with prebiotic and probiotic SO AVIAN PATHOLOGY LA English DT Article ID GRADIENT GEL-ELECTROPHORESIS; MIXED EIMERIA INFECTION; ESSENTIAL OIL BLENDS; 16S RIBOSOMAL-RNA; DIETARY SUPPLEMENTATION; MANNAN-OLIGOSACCHARIDE; DIGESTIVE MICROFLORA; SALMONELLA; POULTRY; COMMUNITIES AB The gastrointestinal tract commensal microbiome is important for host nutrition, health and immunity. Little information is available regarding the role of these commensals at other mucosal surfaces in poultry. Tracheal mucosal surfaces offer sites for first-line health and immunity promotion in broilers, especially under stress-related conditions. The present study is aimed at elucidating the effects of feed supplementations with mannanoligosaccharides (MOS) prebiotic and a probiotic mixture (PM) on the caecal and tracheal microbiome of broilers kept under chronic heat stress (HS; 35 +/- 2 degrees C). Day-old chickens were randomly divided into five treatment groups: thermoneutral control (TN-CONT), HS-CONT, HS-MOS, HS-PM and HS synbiotic (fed MOS and PM). Caecal digesta and tracheal swabs were collected at day 42 and subjected to DNA extraction, followed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and pyrosequencing. The PCR-DGGE dendrograms revealed significant (49.5% similarity coefficients) differences between caecal and tracheal microbiome. Tracheal microbiome pyrosequencing revealed 9 phyla, 17 classes, 34 orders, 68 families and 125 genera, while 11 phyla, 19 classes, 34 orders, 85 families and 165 genera were identified in caeca. An unweighted UniFrac distance metric revealed a distinct clustering pattern (analysis of similarities, P = 0.007) between caecal and tracheal microbiome. Lactobacillus was the most abundant genus in trachea and caeca and was more abundant in caeca and trachea of HS groups compared with the TN-CONT group. Distinct bacterial clades occupied the caecal and tracheal microbiomes, although some bacterial groups overlapped, demonstrating a core microbiome dominated by Lactobacillus. No positive effects of supplementations were observed on abundance of probiotic bacteria. C1 [Sohail, Muhammad U.; Hume, Michael E.; Byrd, James A.; Nisbet, David J.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. [Sohail, Muhammad U.] Govt Coll Univ, Dept Physiol, Faisalabad, Pakistan. [Shabbir, Muhammad Z.; Rehman, Habib] Univ Vet & Anim Sci, Qual Operat Lab, Lahore, Pakistan. [Shabbir, Muhammad Z.; Rehman, Habib] Univ Vet & Anim Sci, Dept Physiol, Lahore, Pakistan. [Ijaz, Ahmad] Imperial Coll Business Studies, Lahore, Pakistan. RP Sohail, MU (reprint author), ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. EM umar.sohail@gcuf.edu.pk OI Sohail, Muhammad Umar/0000-0003-3250-3174; Shabbir, Muhammad Zubair/0000-0002-3562-007X FU ARS-USDA internal funds FX The study was sponsored by ARS-USDA internal funds. Muhammad U. Sohail visited ARS-USDA, College Station, TX, USA, on Higher Education Commission, Pakistan, research grant. NR 31 TC 2 Z9 3 U1 8 U2 42 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0307-9457 EI 1465-3338 J9 AVIAN PATHOL JI Avian Pathol. PD MAR 4 PY 2015 VL 44 IS 2 BP 67 EP 74 DI 10.1080/03079457.2015.1004622 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CC5FM UT WOS:000350384500002 PM 25564364 ER PT J AU Beltran, MS Gerba, CP Fett, AP Luchansky, JB Chaidez, C AF Beltran, Marcela Soto Gerba, Charles P. Fett, Anna Porto Luchansky, John B. Chaidez, Cristobal TI Prevalence and characterization of Listeria monocytogenes, Salmonella and Shiga toxin-producing Escherichia coli isolated from small Mexican retail markets of queso fresco SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH LA English DT Article DE queso fresco; food safety; Escherichia coli; Shiga toxin; Salmonella; Listeria monocytogenes ID SOFT CHEESE; RAW-MILK; PASTEURIZED MILK; UNITED-STATES; STYLE CHEESE; OUTBREAK; O157-H7; IDENTIFICATION; INFECTIONS; FOODS AB Queso fresco (QF) is a handmade cheese consumed and produced in Latin America. In Mexico, QF production is associated with a microbiological risk. The aim of the study was to determine the incidence and characterization of Listeria monocytogenes, Salmonella spp., and Shiga toxin-producing Escherichia coli (STEC) in QF from retail markets of the north-western State of Sinaloa, Mexico, and to assess the effect of physicochemical parameters on Listeria presence. A total of 75 QF samples were obtained. L. monocytogenes, E. coli, and coliforms were detected in 9.3, 94, and 100 %, respectively. Salmonella was not detected. STEC isolates showed virulence genes. Microbial loads were above the maximum values recommended by the Official Mexican Standards. Physicochemical parameters such as water activity (a(w)), moisture content, pH, and salinity played a role in Listeria prevalence in QF. Rigorous control in QF made in Culiacan, Mexico is needed to reduce the risk of foodborne pathogens. C1 [Beltran, Marcela Soto; Gerba, Charles P.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ USA. [Fett, Anna Porto; Luchansky, John B.] USDA ARS, Wyndmoor, PA USA. [Chaidez, Cristobal] CIAD, Culiacan, Mexico. RP Chaidez, C (reprint author), CIAD, Culiacan, Mexico. EM chaqui@ciad.edu.mx NR 42 TC 1 Z9 1 U1 0 U2 17 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0960-3123 EI 1369-1619 J9 INT J ENVIRON HEAL R JI Int. J. Environ. Health Res. PD MAR 4 PY 2015 VL 25 IS 2 BP 140 EP 148 DI 10.1080/09603123.2014.915016 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA CB3MU UT WOS:000349533100003 ER PT J AU Taliercio, E Loveless, T Turano, MJ AF Taliercio, Earl Loveless, Telisa Turano, Marc J. TI Identification of epitopes of the A1aBx and A5A4B3 subunits of glycinin antigenic in three animal species SO FOOD AND AGRICULTURAL IMMUNOLOGY LA English DT Article DE IgE; rabbit; IgG; hybrid striped bass; dog; allergen ID EARLY-WEANED PIGS; BETA-CONGLYCININ; SOYBEAN-MEAL; GROWTH-PERFORMANCE; ARA H-3; ALLERGEN; HYPERSENSITIVITY; PROTEINS; DOGS; SOY AB Soybean meal is commonly added to a variety of animal feeds to supplement protein sources and to optimise growth. While soybean protein is a valuable food supplement it has been recognised as an important food allergen. The soybean seed storage protein, glycinin, has been identified as an allergen. A tiled peptide array of the A1aBx and A5A4B3 subunits of glycinin was screened to identify the epitopes that bind antibodies from multiple species. We have identified four regions in these two glycinin subunits that are antigenic in most or all of the species tested. One region is implicated in an allergic response in dogs by the dog's ability to bind IgE. Three regions overlap or abut regions that are similar to allergenic epitopes in peanut. It will be critical to identify immunogenic regions able to cause allergies to soy in order to prioritise them for mitigation. C1 [Taliercio, Earl; Loveless, Telisa] USDA ARS, Raleigh, NC 27695 USA. [Turano, Marc J.] N Carolina State Univ, North Carolina Sea Grant, Raleigh, NC 27695 USA. RP Taliercio, E (reprint author), USDA ARS, Raleigh, NC 27695 USA. EM Earl.Taliercio@ars.usda.gov NR 21 TC 0 Z9 1 U1 2 U2 22 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0954-0105 EI 1465-3443 J9 FOOD AGR IMMUNOL JI Food Agric. Immunol. PD MAR 4 PY 2015 VL 26 IS 2 BP 271 EP 281 DI 10.1080/09540105.2014.906566 PG 11 WC Chemistry, Applied; Food Science & Technology; Immunology; Toxicology SC Chemistry; Food Science & Technology; Immunology; Toxicology GA AX8JO UT WOS:000347156100011 ER PT J AU Freilich, S Lev, S Gonda, I Reuveni, E Portnoy, V Oren, E Lohse, M Galpaz, N Bar, E Tzuri, G Wissotsky, G Meir, A Burger, J Tadmor, Y Schaffer, A Fei, ZJ Giovannoni, J Lewinsohn, E Katzir, N AF Freilich, Shiri Lev, Shery Gonda, Itay Reuveni, Eli Portnoy, Vitaly Oren, Elad Lohse, Marc Galpaz, Navot Bar, Einat Tzuri, Galil Wissotsky, Guy Meir, Ayala Burger, Joseph Tadmor, Yaakov Schaffer, Arthur Fei, Zhangjun Giovannoni, James Lewinsohn, Efraim Katzir, Nurit TI Systems approach for exploring the intricate associations between sweetness, color and aroma in melon fruits SO BMC PLANT BIOLOGY LA English DT Article DE Fruit quality; Specialized metabolites; Metabolomic; Transcriptomic; Correlation analysis; Recombinant inbred lines ID CUCUMIS-MELO; TOMATO FRUIT; GENE-EXPRESSION; FUNCTIONAL-CHARACTERIZATION; MOLECULAR MARKERS; CHARENTAIS MELON; ENZYME-ACTIVITY; L. FRUIT; RNA-SEQ; ACCUMULATION AB Background: Melon (Cucumis melo) fruits exhibit phenotypic diversity in several key quality determinants such as taste, color and aroma. Sucrose, carotenoids and volatiles are recognized as the key compounds shaping the above corresponding traits yet the full network of biochemical events underlying their synthesis have not been comprehensively described. To delineate the cellular processes shaping fruit quality phenotypes, a population of recombinant inbred lines (RIL) was used as a source of phenotypic and genotypic variations. In parallel, ripe fruits were analyzed for both the quantified level of 77 metabolic traits directly associated with fruit quality and for RNA-seq based expression profiles generated for 27,000 unigenes. First, we explored inter-metabolite association patterns; then, we described metabolites versus gene association patterns; finally, we used the correlation-based associations for predicting uncharacterized synthesis pathways. Results: Based on metabolite versus metabolite and metabolite versus gene association patterns, we divided metabolites into two key groups: a group including ethylene and aroma determining volatiles whose accumulation patterns are correlated with the expression of genes involved in the glycolysis and TCA cycle pathways; and a group including sucrose and color determining carotenoids whose accumulation levels are correlated with the expression of genes associated with plastid formation. Conclusions: The study integrates multiple processes into a genome scale perspective of cellular activity. This lays a foundation for deciphering the role of gene markers associated with the determination of fruit quality traits. C1 [Freilich, Shiri; Lev, Shery; Gonda, Itay; Reuveni, Eli; Portnoy, Vitaly; Oren, Elad; Galpaz, Navot; Bar, Einat; Tzuri, Galil; Wissotsky, Guy; Meir, Ayala; Burger, Joseph; Tadmor, Yaakov; Schaffer, Arthur; Lewinsohn, Efraim; Katzir, Nurit] Agr Res Org, Newe Yaar Res Ctr, IL-30095 Ramat Yishay, Israel. [Lohse, Marc] Targenomix GmbH, Potsdam, Germany. [Galpaz, Navot] Migal Res Inst, IL-11016 Kiryat Shmona, Israel. [Fei, Zhangjun; Giovannoni, James] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. [Fei, Zhangjun; Giovannoni, James] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Freilich, S (reprint author), Agr Res Org, Newe Yaar Res Ctr, IL-30095 Ramat Yishay, Israel. EM shiri@volcani.agri.gov.il FU BARD [IS-4223-09C]; Chief Scientist of the Ministry of Agriculture of Israel [261-1049-14] FX This research was supported by Research Grant Award No. IS-4223-09C from BARD, and by the Chief Scientist of the Ministry of Agriculture of Israel (project no. 261-1049-14). NR 71 TC 3 Z9 3 U1 10 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAR 3 PY 2015 VL 15 AR 71 DI 10.1186/s12870-015-0449-x PG 16 WC Plant Sciences SC Plant Sciences GA CM7YW UT WOS:000357913400001 PM 25887588 ER PT J AU Plevin, RJ Beckman, J Golub, AA Witcover, J O'Hare, M AF Plevin, Richard J. Beckman, Jayson Golub, Alla A. Witcover, Julie O'Hare, Michael TI Carbon Accounting and Economic Model Uncertainty of Emissions from Biofuels-Induced Land Use Change SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID GREENHOUSE-GAS EMISSIONS; TRADE; RESPONSES; ETHANOL AB Few of the numerous published studies of the emissions from biofuels-induced "indirect" land use change (ILUC) attempt to propagate and quantify uncertainty, and those that have done so have restricted their analysis to a portion of the modeling systems used. In this study, we pair a global, computable general equilibrium model with a model of greenhouse gas emissions from land-use change to quantify the parametric uncertainty in the paired modeling systems estimates of greenhouse gas emissions from ILUC induced by expanded production of three biofuels. We find that for the three fuel systems examined-US corn ethanol, Brazilian sugar cane ethanol, and US soybean biodiesel-95% of the results occurred within +/- 20 g CO(2)e MJ(-1) of the mean (coefficient of variation of 20-45%), with economic model parameters related to crop yield and the productivity of newly converted cropland (from forestry and pasture) contributing most of the variance in estimated ILUC emissions intensity. Although the experiments performed here allow us to characterize parametric uncertainty, changes to the model structure have the potential to shift the mean by tens of grams of CO(2)e per megajoule and further broaden distributions for ILUC emission intensities. C1 [Plevin, Richard J.; Witcover, Julie] Univ Calif Davis, Inst Transportat Studies, Davis, CA 95616 USA. [Beckman, Jayson] ERS, USDA, Washington, DC 20250 USA. [Golub, Alla A.] Purdue Univ, Ctr Global Trade Anal, Dept Agr Econ, W Lafayette, IN 47907 USA. [O'Hare, Michael] Univ Calif Berkeley, Goldman Sch Publ Policy, Berkeley, CA 94720 USA. RP Plevin, RJ (reprint author), Univ Calif Davis, Inst Transportat Studies, Davis, CA 95616 USA. EM plevin@ucdavis.edu FU California Air Resources Board; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX Portions of this research were funded by the California Air Resources Board. This research used resources of the National Energy Research Scientific Computing Center, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Authors thank the anonymous reviewers. The article represents the views of the authors and not necessarily of CARB, US DOE, or US Department of Agriculture. NR 48 TC 14 Z9 14 U1 10 U2 35 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAR 3 PY 2015 VL 49 IS 5 BP 2656 EP 2664 DI 10.1021/es505481 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CC8IH UT WOS:000350611100014 PM 25622072 ER PT J AU Harrington, CA Gould, PJ AF Harrington, Constance A. Gould, Peter J. TI Tradeoffs between chilling and forcing in satisfying dormancy requirements for Pacific Northwest tree species SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE chilling; forcing; dormancy; possibility line; budburst; parallel model ID DOUGLAS-FIR SEEDLINGS; BUD DORMANCY; FROST DAMAGE; TEMPERATE REGIONS; PICEA-SITCHENSIS; CLIMATIC-CHANGE; COLD-STORAGE; PINUS-TAEDA; PHENOLOGY; BUDBURST AB Many temperate and boreal tree species have a chilling requirement, that is, they need to experience cold temperatures during fall and winter to burst bud normally in the spring. Results from trials with 11 Pacific Northwest tree species are consistent with the concept that plants can accumulate both chilling and forcing units simultaneously during the dormant season and they exhibit a tradeoff between amount of forcing and chilling. That is, the parallel model of chilling and forcing was effective in predicting budburst and well chilled plants require less forcing for bud burst than plants which have received less chilling. Genotypes differed in the shape of the possibility line which describes the quantitative tradeoff between chilling and forcing units. Plants which have an obligate chilling requirement (Douglas-fir, western hemlock, western larch, pines, and true firs) and received no or very low levels of chilling did not burst bud normally even with long photoperiods. Pacific madrone and western redcedar benefited from chilling in terms of requiring less forcing to promote bud burst but many plants burst bud normally without chilling. Equations predicting budburst were developed for each species in our trials for a portion of western North America under current climatic conditions and for 2080. Mean winter temperature was predicted to increase 3.2-5.5 degrees C and this change resulted in earlier predicted budburst for Douglas-fir throughout much of our study area (up to 74 days earlier) but later budburst in some southern portions of its current range (up to 48 days later) as insufficient chilling is predicted to occur. Other species all had earlier predicted dates of budburst by 2080 than currently. Recent warming trends have resulted in earlier budburst for some woody plant species; however, the substantial winter warming predicted by some climate models will reduce future chilling in some locations such that budburst will not consistently occur earlier. C1 [Harrington, Constance A.] US Forest Serv, USDA, Pacific Northwest Res Stn, Olympia, WA 98512 USA. [Gould, Peter J.] Washington State Dept Nat Resources, Olympia, WA USA. RP Harrington, CA (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM charrington@fs.fed.us FU Office, Bureau of Land Management FX We thank the Oregon State Office, Bureau of Land Management for financial support, the Washington Department of Natural Resources Lt. Mike Webster Nursery for donating supplies, Marianne Elliott, Washington State University for donating the madrone seedlings, Teresa Vail, Leslie Brodie, and David Thornton for assistance in conducting the study, and Kevin Ford for assistance with the maps. NR 58 TC 4 Z9 4 U1 3 U2 33 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 3 PY 2015 VL 6 AR 120 DI 10.3389/fpls.2015.00120 PG 12 WC Plant Sciences SC Plant Sciences GA CD1VD UT WOS:000350861700001 PM 25784922 ER PT J AU Chitwood, MC Lashley, MA Kilgo, JC Pollock, KH Moorman, CE DePerno, CS AF Chitwood, M. Colter Lashley, Marcus A. Kilgo, John C. Pollock, Kenneth H. Moorman, Christopher E. DePerno, Christopher S. TI Do Biological and Bedsite Characteristics Influence Survival of Neonatal White-Tailed Deer? SO PLOS ONE LA English DT Article ID COYOTE PREDATION; NORTH-AMERICA; FAWNS; MORTALITY; POPULATION; MINNESOTA; NUTRITION; RATES; RISK; ELK AB Coyotes recently expanded into the eastern U.S. and potentially have caused localized white-tailed deer population declines. Research has focused on quantifying coyote predation on neonates, but little research has addressed the potential influence of bedsite characteristics on survival. In 2011 and 2012, we radiocollared 65 neonates, monitored them intensively for 16 weeks, and assigned mortality causes. We used Program MARK to estimate survival to 16 weeks and included biological covariates (i.e., sex, sibling status [whether or not it had a sibling], birth weight, and Julian date of birth). Survival to 16 weeks was 0.141 (95% CI = 0.075-0.249) and the top model included only sibling status, which indicated survival was lower for neonates that had a sibling. Predation was the leading cause of mortality (35 of 55; 64%) and coyotes were responsible for the majority of depredations (30 of 35; 86%). Additionally, we relocated neonates for the first 10 days of life and measured distance to firebreak, visual obstruction, and plant diversity at bedsites. Survival of predation to 10 days (0.726; 95% CI = 0.586-0.833) was weakly associated with plant diversity at bedsites but not related to visual obstruction. Our results indicate that neonate survival was low and coyote predation was an important source of mortality, which corroborates several recent studies from the region. Additionally, we detected only weak support for bedsite cover as a covariate to neonate survival, which indicates that mitigating effects of coyote predation on neonates may be more complicated than simply managing for increased hiding cover. C1 [Chitwood, M. Colter; Lashley, Marcus A.; Moorman, Christopher E.; DePerno, Christopher S.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Kilgo, John C.] US Forest Serv, USDA, Southern Res Stn, New Ellenton, SC USA. [Pollock, Kenneth H.] N Carolina State Univ, Dept Appl Ecol, Raleigh, NC 27695 USA. RP Chitwood, MC (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. EM colter_chitwood@ncsu.edu FU United States Department of Defense; Fort Bragg Wildlife Branch FX The authors thank the United States Department of Defense and Fort Bragg Wildlife Branch for financial contributions to this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 6 Z9 6 U1 3 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 3 PY 2015 VL 10 IS 3 AR e0119070 DI 10.1371/journal.pone.0119070 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC4HV UT WOS:000350314700053 PM 25734333 ER PT J AU Terhzaz, S Teets, NM Cabrero, P Henderson, L Ritchie, MG Nachman, RJ Dow, JAT Denlinger, DL Davies, SA AF Terhzaz, Selim Teets, Nicholas M. Cabrero, Pablo Henderson, Louise Ritchie, Michael G. Nachman, Ronald J. Dow, Julian A. T. Denlinger, David L. Davies, Shireen-A. TI Insect capa neuropeptides impact desiccation and cold tolerance SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE environmental stress; insects; neuropeptides; capa; desiccation and cold tolerance ID CHILL-COMA RECOVERY; DROSOPHILA-MELANOGASTER; ION HOMEOSTASIS; CARDIOACCELERATORY PEPTIDE; LOCUSTA-MIGRATORIA; FREEZE TOLERANCE; LOW-TEMPERATURE; WATER-BALANCE; V-ATPASE; STRESS AB The success of insects is linked to their impressive tolerance to environmental stress, but little is known about how such responses are mediated by the neuroendocrine system. Here we show that the capability (capa) neuropeptide gene is a desiccation-and cold stress-responsive gene in diverse dipteran species. Using targeted in vivo gene silencing, physiological manipulations, stress-tolerance assays, and rationally designed neuropeptide analogs, we demonstrate that the Drosophila melanogaster capa neuropeptide gene and its encoded peptides alter desiccation and cold tolerance. Knockdown of the capa gene increases desiccation tolerance but lengthens chill coma recovery time, and injection of capa peptide analogs can reverse both phenotypes. Immunohistochemical staining suggests that capa accumulates in the capa-expressing Va neurons during desiccation and nonlethal cold stress but is not released until recovery from each stress. Our results also suggest that regulation of cellular ion and water homeostasis mediated by capa peptide signaling in the insect Malpighian (renal) tubules is a key physiological mechanism during recovery from desiccation and cold stress. This work augments our understanding of how stress tolerance is mediated by neuroendocrine signaling and illustrates the use of rationally designed peptide analogs as agents for disrupting protective stress tolerance. C1 [Terhzaz, Selim; Cabrero, Pablo; Henderson, Louise; Dow, Julian A. T.; Davies, Shireen-A.] Univ Glasgow, Coll Med Vet & Life Sci, Inst Mol Cell & Syst Biol, Glasgow G12 8QQ, Lanark, Scotland. [Teets, Nicholas M.; Denlinger, David L.] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA. [Teets, Nicholas M.; Denlinger, David L.] Ohio State Univ, Dept Ecol Evolut & Organismal Biol, Columbus, OH 43210 USA. [Ritchie, Michael G.] Univ St Andrews, Ctr Biol Divers, Sch Biol, St Andrews KY16 9TS, Fife, Scotland. [Nachman, Ronald J.] USDA, Southern Plains Agr Res Ctr, Insect Control & Cotton Dis Res Unit, College Stn, TX 77845 USA. RP Terhzaz, S (reprint author), Univ Glasgow, Coll Med Vet & Life Sci, Inst Mol Cell & Syst Biol, Glasgow G12 8QQ, Lanark, Scotland. EM selim.terhzaz@glasgow.ac.uk; denlinger.1@osu.edu; shireen.davies@glasgow.ac.uk RI Ritchie, Michael/F-7055-2013; Teets, Nicholas/H-7386-2013; OI Ritchie, Michael/0000-0001-7913-8675; Teets, Nicholas/0000-0003-0963-7457; Cabrero, Pablo/0000-0003-2141-7852 FU UK Biotechnology and Biological Sciences Research Council [BB/G020620, BB/L002647/1]; US Department of Agriculture/Department of Defense Deployed War Fighters Protection Grant Initiative [6202-22000-029-00D]; US-Israel Binational Agricultural Research and Development Fund (BARD) [IS-4205-09C]; National Science Foundation [IOS-0840772] FX We thank K. Lukowiak, W. Bendena, and A. Dornan for discussions and comments on the manuscript; G. Overend and L. Ranford-Cartwright for mosquitoes; W. Etges for D. mojavensis; and D. Parker for D. montana bioinformatics. This work was funded by grants from the UK Biotechnology and Biological Sciences Research Council (BB/G020620 and BB/L002647/1) (to S.-A.D., J.A.T.D., and S.T.); US Department of Agriculture/Department of Defense Deployed War Fighters Protection Grant Initiative (#6202-22000-029-00D) and US-Israel Binational Agricultural Research and Development Fund (BARD) (IS-4205-09C) (R.J.N.); and the National Science Foundation (IOS-0840772) (D.L.D.). NR 51 TC 14 Z9 14 U1 5 U2 44 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 3 PY 2015 VL 112 IS 9 BP 2882 EP 2887 DI 10.1073/pnas.1501518112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC3DK UT WOS:000350224900070 PM 25730885 ER PT J AU Mathieu, C Moreno, V Pedersen, J Jeria, J Agredo, M Gutierrez, C Garcia, A Vasquez, M Avalos, P Retamal, P AF Mathieu, Christian Moreno, Valentina Pedersen, Janice Jeria, Julissa Agredo, Michel Gutierrez, Cristian Garcia, Alfonso Vasquez, Marcela Avalos, Patricia Retamal, Patricio TI Avian Influenza in wild birds from Chile, 2007-2009 SO VIRUS RESEARCH LA English DT Article DE Influenza; Viruses; Wild birds; Chile ID TIME RT-PCR; VIRUS; WATERFOWL; ARGENTINA; LINEAGE; POULTRY AB Aquatic and migratory birds, the main reservoir hosts of avian influenza viruses including those with high pathogenic potential, are the wildlife species with the highest risk for viral dissemination across countries and continents. In 2002, the Chilean poultry industry was affected with a highly pathogenic avian influenza strain, which created economic loss and triggered the establishment of a surveillance program in wild birds. This effort consisted of periodic samplings of sick or suspicious animals found along the coast and analyses with standardized techniques for detection of influenza A virus. The aim of this work is to report the detection of three avian influenza strains (H13N2, H5N9, H13N9) in gulls from Chile between 2007-2009, which nucleotide sequences showed highest similitudes to viruses detected in wild birds from North America. These results suggest a dissemination route for influenza viruses along the coasts of Americas. Migratory and synanthropic behaviors of birds included in this study support continued monitoring of avian influenza viruses isolated from wild birds in The Americas and the establishment of biosecurity practices in farms. (C) 2015 Elsevier B.V. All rights reserved. C1 [Mathieu, Christian; Moreno, Valentina; Jeria, Julissa; Agredo, Michel; Gutierrez, Cristian; Garcia, Alfonso; Vasquez, Marcela; Avalos, Patricia] Serv Agr & Ganadero Chile SAG, Santiago, Chile. [Pedersen, Janice] Natl Vet Serv Labs, Avian Sect Diagnost Virol Lab, Ames, IA 50010 USA. [Retamal, Patricio] Univ Chile, Fac Ciencias Vet & Pecuarias, Santiago, Chile. RP Retamal, P (reprint author), Univ Chile, Fac Ciencias Vet & Pecuarias, Av Sta Rosa 11735, Santiago, Chile. EM pretamal@uchile.cl RI Retamal, Patricio/G-3054-2013 OI Retamal, Patricio/0000-0001-8597-9550 FU Exotic Avian Diseases Surveillance Program FX This work was supported by the Exotic Avian Diseases Surveillance Program. NR 18 TC 6 Z9 6 U1 0 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD MAR 2 PY 2015 VL 199 BP 42 EP 45 DI 10.1016/j.virusres.2015.01.008 PG 4 WC Virology SC Virology GA CE4JE UT WOS:000351796100005 PM 25602438 ER PT J AU Zhao, CY Escalante, LN Chen, H Benatti, TR Qu, JX Chellapilla, S Waterhouse, RM Wheeler, D Andersson, MN Bao, RY Batterton, M Behura, SK Blankenburg, KP Caragea, D Carolan, JC Coyle, M El-Bouhssini, M Francisco, L Friedrich, M Gill, N Grace, T Grimmelikhuijzen, CJP Han, Y Hauser, F Herndon, N Holder, M Ioannidis, P Jackson, L Javaid, M Jhangiani, SN Johnson, AJ Kalra, D Korchina, V Kovar, CL Lara, F Lee, SL Liu, XM Lofstedt, C Mata, R Mathew, T Muzny, DM Nagar, S Nazareth, LV Okwuonu, G Ongeri, F Perales, L Peterson, BF Pu, LL Robertson, HM Schemerhorn, BJ Scherer, SE Shreve, JT Simmons, D Subramanyam, S Thornton, RL Xue, K Weissenberger, GM Williams, CE Worley, KC Zhu, DH Zhu, YM Harris, MO Shukle, RH Werren, JH Zdobnov, EM Chen, MS Brown, SJ Stuart, JJ Richards, S AF Zhao, Chaoyang Escalante, Lucia Navarro Chen, Hang Benatti, Thiago R. Qu, Jiaxin Chellapilla, Sanjay Waterhouse, Robert M. Wheeler, David Andersson, Martin N. Bao, Riyue Batterton, Matthew Behura, Susanta K. Blankenburg, Kerstin P. Caragea, Doina Carolan, James C. Coyle, Marcus El-Bouhssini, Mustapha Francisco, Liezl Friedrich, Markus Gill, Navdeep Grace, Tony Grimmelikhuijzen, Cornelis J. P. Han, Yi Hauser, Frank Herndon, Nicolae Holder, Michael Ioannidis, Panagiotis Jackson, LaRonda Javaid, Mehwish Jhangiani, Shalini N. Johnson, Alisha J. Kalra, Divya Korchina, Viktoriya Kovar, Christie L. Lara, Fremiet Lee, Sandra L. Liu, Xuming Loefstedt, Christer Mata, Robert Mathew, Tittu Muzny, Donna M. Nagar, Swapnil Nazareth, Lynne V. Okwuonu, Geoffrey Ongeri, Fiona Perales, Lora Peterson, Brittany F. Pu, Ling-Ling Robertson, Hugh M. Schemerhorn, Brandon J. Scherer, Steven E. Shreve, Jacob T. Simmons, DeNard Subramanyam, Subhashree Thornton, Rebecca L. Xue, Kun Weissenberger, George M. Williams, Christie E. Worley, Kim C. Zhu, Dianhui Zhu, Yiming Harris, Marion O. Shukle, Richard H. Werren, John H. Zdobnov, Evgeny M. Chen, Ming-Shun Brown, Susan J. Stuart, Jeffery J. Richards, Stephen TI A Massive Expansion of Effector Genes Underlies Gall-Formation in the Wheat Pest Mayetiola destructor SO CURRENT BIOLOGY LA English DT Article ID PLANT IMMUNE-SYSTEM; HESSIAN FLY; GENOME; PROTEINS; BACTERIAL; FUNGAL; ENDOSYMBIONT; EXPRESSION; PREDICTION; RESISTANCE AB Gall-forming arthropods are highly specialized herbivores that, in combination with their hosts, produce extended phenotypes with unique morphologies [1]. Many are economically important, and others have improved our understanding of ecology and adaptive radiation [2]. However, the mechanisms that these arthropods use to induce plant galls are poorly understood. We sequenced the genome of the Hessian fly (Mayetiola destructor; Diptera: Cecidomyiidae), a plant parasitic gall midge and a pest of wheat (Triticum spp.), with the aim of identifying genic modifications that contribute to its plant-parasitic lifestyle. Among several adaptive modifications, we discovered an expansive reservoir of potential effector proteins. Nearly 5% of the 20,163 predicted gene models matched putative effector gene transcripts present in the M. destructor larval salivary gland. Another 466 putative effectors were discovered among the genes that have no sequence similarities in other organisms. The largest known arthropod gene family (family SSGP-71) was also discovered within the effector reservoir. SSGP-71 proteins lack sequence homologies to other proteins, but their structures resemble both ubiquitin E3 ligases in plants and E3-ligase-mimicking effectors in plant pathogenic bacteria. SSGP-71 proteins and wheat Skp proteins interact in vivo. Mutations in different SSGP-71 genes avoid the effector-triggered immunity that is directed by the wheat resistance genes H6 and H9. Results point to effectors as the agents responsible for arthropod-induced plant gall formation. C1 [Zhao, Chaoyang; Escalante, Lucia Navarro; Benatti, Thiago R.; Peterson, Brittany F.; Shreve, Jacob T.; Stuart, Jeffery J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47097 USA. [Chen, Hang] Kansas State Univ, Dept Entomol, Manhattan, KS 66056 USA. [Chen, Hang] Chinese Acad Forestry, Res Inst Resource Insects, Bailongsi 650224, Kunming, Peoples R China. [Qu, Jiaxin; Batterton, Matthew; Blankenburg, Kerstin P.; Coyle, Marcus; Francisco, Liezl; Han, Yi; Holder, Michael; Jackson, LaRonda; Javaid, Mehwish; Jhangiani, Shalini N.; Korchina, Viktoriya; Kovar, Christie L.; Lara, Fremiet; Lee, Sandra L.; Mata, Robert; Mathew, Tittu; Muzny, Donna M.; Nazareth, Lynne V.; Okwuonu, Geoffrey; Ongeri, Fiona; Perales, Lora; Pu, Ling-Ling; Scherer, Steven E.; Simmons, DeNard; Thornton, Rebecca L.; Weissenberger, George M.; Worley, Kim C.; Zhu, Dianhui; Zhu, Yiming; Richards, Stephen] Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, Houston, TX 77030 USA. [Chellapilla, Sanjay; Caragea, Doina; Grace, Tony; Herndon, Nicolae; Kalra, Divya; Nagar, Swapnil; Brown, Susan J.] Kansas State Univ, Div Biol, KSU Bioinfornnat Ctr, Manhattan, KS 66506 USA. [Waterhouse, Robert M.; Ioannidis, Panagiotis; Zdobnov, Evgeny M.] Univ Geneva, Sch Med, Dept Genet Med & Dev, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.; Ioannidis, Panagiotis; Zdobnov, Evgeny M.] Swiss Inst Bioinformat, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.] MIT, Comp Sci, Cambridge, MA 02139 USA. [Waterhouse, Robert M.] MIT, Artificial Intelligence Lab, Cambridge, MA 02139 USA. [Waterhouse, Robert M.] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA. [Wheeler, David; Werren, John H.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA. [Andersson, Martin N.; Loefstedt, Christer] Lund Univ, Dept Biol, S-22362 Lund, Sweden. [Bao, Riyue] Univ Chicago, Ctr Res Informat, Div Biol Sci, Bioinformat Core, Chicago, IL 60637 USA. [Bao, Riyue; Friedrich, Markus] Wayne State Univ, Dept Biol Sci, Detroit, MI 48202 USA. [Behura, Susanta K.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Caragea, Doina] Kansas State Univ, Dept Comp & Informat Sci, Manhattan, KS 66506 USA. [Carolan, James C.] Natl Univ Ireland Maynooth, Dept Biol, Maynooth, Kildare, Ireland. [El-Bouhssini, Mustapha] Int Ctr Agr Res Dry Areas, Rabat, Morocco. [Gill, Navdeep] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. [Grimmelikhuijzen, Cornelis J. P.; Hauser, Frank] Univ Copenhagen, Ctr Funct & Comparat Insect Genom, DK-2100 Copenhagen, Denmark. [Johnson, Alisha J.; Schemerhorn, Brandon J.; Shukle, Richard H.] Purdue Univ, USDA ARS, Dept Entomol, W Lafayette, IN 47907 USA. [Liu, Xuming; Chen, Ming-Shun] Kansas State Univ, Dept Entomol, USDA ARS, Manhattan, KS 66056 USA. [Robertson, Hugh M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Subramanyam, Subhashree] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Xue, Kun] Minzu Univ, Coll Life & Environm Sci, Beijing 100081, Peoples R China. [Williams, Christie E.] Purdue Univ, Dept Agron, USDA ARS, W Lafayette, IN 47907 USA. [Harris, Marion O.] N Dakota State Univ, Dept Entomol, Fargo, ND 58108 USA. RP Richards, S (reprint author), Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA. EM stephenr@bcm.edu RI Hauser, Frank/M-2952-2014; Waterhouse, Robert/A-1858-2010; Zdobnov, Evgeny/K-1133-2012; Ioannidis, Panagiotis/I-4281-2014; OI Hauser, Frank/0000-0001-5563-2345; Waterhouse, Robert/0000-0003-4199-9052; Ioannidis, Panagiotis/0000-0003-0939-6745; Zhao, Chaoyang/0000-0003-3378-3163; Grimmelikhuijzen, Cornelis/0000-0001-6486-2046 FU USDA-NIFA AFRI grant [2008-35302-18816, 2010-03741]; NSF [DEB0821936, DEB1257053]; Swedish Research Council (V.R.); Royal Physiographic Society; Marie Curie International Outgoing Fellowship [PIOF-GA-2011-303312]; Swiss National Science Foundation [31003A-125350, 31003A-143936] FX Genome sequencing was. supported by USDA-NIFA AFRI grant 2008-35302-18816 to S.R. Virulence mutation mapping performed in the J.J.S. lab was funded by USDA-NIFA AFRI grant 2010-03741 to J.S. Work by J.H.W. and D.W. was supported by NSF DEB0821936 and DEB1257053. M.N.A. and C.L. were funded by grants from the Swedish Research Council (V.R.) and the Royal Physiographic Society. R.M.W. was supported by Marie Curie International Outgoing Fellowship PIOF-GA-2011-303312. R.M.W. and P.I. were supported by Swiss National Science Foundation awards 31003A-125350 and 31003A-143936 to E.M.Z. Denise Caldwell and Andrew Katz (Purdue University) provided the photographs shown in Figure 1. NR 41 TC 27 Z9 28 U1 4 U2 47 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0960-9822 EI 1879-0445 J9 CURR BIOL JI Curr. Biol. PD MAR 2 PY 2015 VL 25 IS 5 BP 613 EP 620 DI 10.1016/j.cub.2014.12.057 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA CC9RP UT WOS:000350708800024 PM 25660540 ER PT J AU Enenkel, M See, LD Bonifacio, R Boken, V Chaney, N Vinck, P You, LZ Dutra, E Anderson, M AF Enenkel, Markus See, Linda Bonifacio, Rogerio Boken, Vijendra Chaney, Nathaniel Vinck, Patrick You, Liangzhi Dutra, Emanuel Anderson, Martha TI Drought and food security - Improving decision-support via new technologies and innovative collaboration SO GLOBAL FOOD SECURITY-AGRICULTURE POLICY ECONOMICS AND ENVIRONMENT LA English DT Article DE Drought; Food Security; Remote Sensing; Forecasting; Vulnerability; Crowd Sourcing ID SOIL-MOISTURE; VARIABILITY AB Governments, aid organizations and people affected by drought are struggling to mitigate the resulting impact on both water resources and crops. In this paper we focus on improved decision-support for agricultural droughts that threaten the livelihoods of people living in vulnerable regions. We claim that new strategic partnerships are required to link scientific findings to actual user requirements of governments and aid organizations and to turn data streams into useful information for decision-support. Furthermore, we list several promising approaches, ranging from the integration of satellite-derived soil moisture measurements that link atmospheric processes to anomalies on the land surface to improved long-range weather predictions and mobile applications. The latter can be used for the dissemination of relevant information, but also for validating satellite-derived datasets or for collecting additional information about socio-economic vulnerabilities. Ideally, the consequence is a translation of early warning into local action, strengthening disaster preparedness and avoiding the need for large-scale external support. (C) 2014 The Authors. Published by Elsevier B.V. C1 [Enenkel, Markus] Vienna Univ Technol, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. [See, Linda] Int Inst Appl Syst Anal, Ecosyst Serv & Management Grp, A-2361 Laxenburg, Austria. [Bonifacio, Rogerio] Vulnerabil Assessment & Mapping Unit, World Food Programme, Rome, Italy. [Boken, Vijendra] Univ Nebraska Kearney, Dept Geog & Earth Sci, Kearney, NE 68849 USA. [Chaney, Nathaniel] Princeton Univ, Terr Hydrol Res Grp, Princeton, NJ 08544 USA. [Vinck, Patrick] Harvard Univ, Sch Publ Hlth Harvard Humanitarian Initiat, Cambridge, MA 02138 USA. [You, Liangzhi] Int Food Policy Res Inst, Environm & Prod Technol, Washington, DC 20036 USA. [Dutra, Emanuel] European Ctr Medium Range Weather Forecasts, Reading RG2 9AX, Berks, England. [Anderson, Martha] USDA, Hydrol & Remote Sensing Lab, Agr Res Serv, Beltsville, MD 20705 USA. RP Enenkel, M (reprint author), Vienna Univ Technol, Dept Geodesy & Geoinformat, Gusshausstr 27-29, A-1040 Vienna, Austria. EM markus.enenkel@geo.tuwien.ac.at RI Dutra, Emanuel/A-3774-2010; OI Dutra, Emanuel/0000-0002-0643-2643; Anderson, Martha/0000-0003-0748-5525 FU Austrian Space Applications Programme (ASAP10) of the Austrian Research Promotion Agency [4277353]; SATIDA (Satellite Technologies for Improved Drought-Risk Assessment) FX This research was supported and funded within the framework of SATIDA (Satellite Technologies for Improved Drought-Risk Assessment). SATIDA is a project within the Austrian Space Applications Programme (ASAP10) of the Austrian Research Promotion Agency (project number 4277353). NR 17 TC 5 Z9 5 U1 3 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2211-9124 J9 GLOB FOOD SECUR-AGR JI Glob. Food Secur.-Agric.Policy PD MAR PY 2015 VL 4 BP 51 EP 55 DI 10.1016/j.gfs.2014.08.005 PG 5 WC Food Science & Technology SC Food Science & Technology GA CX8EE UT WOS:000365934600008 ER PT J AU Sagers, CL Londo, JP Bautista, N Lee, EH Watrud, LS King, G AF Sagers, Cynthia L. Londo, Jason P. Bautista, Nonnie Lee, Edward Henry Watrud, Lidia S. King, George TI Benefits of Transgenic Insect Resistance in Brassica Hybrids under Selection SO AGRONOMY-BASEL LA English DT Article DE Brassica; Bt Cry1Ac; feral species; herbivory; Plutella xylostella; risk assessment; weed evolution ID GREEN-FLUORESCENT PROTEIN; GENE FLOW; WILD SUNFLOWERS; OILSEED RAPE; NAPUS; HYBRIDIZATION; EXPRESSION; COSTS; POPULATIONS; EVOLUTION AB Field trials of transgenic crops may result in unintentional transgene flow to compatible crop, native, and weedy species. Hybridization outside crop fields may create novel forms with potential negative outcomes for wild and weedy plant populations. We report here the outcome of large outdoor mesocosm studies with canola (Brassica napus), transgenic canola, a sexually compatible weed B. rapa, and their hybrids. Brassica rapa was hybridized with canola and canola carrying a transgene for herbivore resistance (Bt Cry1Ac) and grown in outdoor mesocosms under varying conditions of competition and insect herbivory. Treatment effects differed significantly among genotypes. Hybrids were larger than all other genotypes, and produced more seeds than the B. rapa parent. Under conditions of heavy herbivory, plants carrying the transgenic resistance were larger and produced more seeds than non-transgenic plants. Pollen derived gene flow from transgenic canola to B. rapa varied between years (5%-22%) and was not significantly impacted by herbivory. These results confirm that canola-weed hybrids benefit from transgenic resistance and are aggressive competitors with congeneric crops and ruderals. Because some crop and crop-weed hybrids may be competitively superior, escapees may alter the composition and ecological functions of plant communities near transgenic crop fields. C1 [Sagers, Cynthia L.] Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA. [Londo, Jason P.] ARS, USDA, Geneva, NY 14456 USA. [Bautista, Nonnie] Univ Philippines Los Banos, Inst Biol Sci, Div Plant Biol, Laguna 4031, Philippines. [Lee, Edward Henry; Watrud, Lidia S.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. [King, George] CSS Dynamac, Corvallis, OR 97333 USA. RP Sagers, CL (reprint author), Univ Arkansas, Dept Biol Sci, Fayetteville, AR 72701 USA. EM csagers@uark.edu; jason.londo@ars.usda.gov; nsbautista@yahoo.com; lee.ehenry@epa.gov; watrud.lidia@epa.gov; king.george@epa.gov NR 35 TC 2 Z9 2 U1 4 U2 6 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD MAR PY 2015 VL 5 IS 1 BP 21 EP 34 DI 10.3390/agronomy5010021 PG 14 WC Agronomy SC Agriculture GA CV4IH UT WOS:000364229900001 ER PT J AU McHugh, T AF McHugh, Tara TI UV Processing of Mushrooms Enhances Vitamin D Content SO FOOD TECHNOLOGY LA English DT Editorial Material C1 USDA ARS, Albany, CA 94710 USA. RP McHugh, T (reprint author), USDA ARS, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD MAR PY 2015 VL 69 IS 3 BP 74 EP 76 PG 3 WC Food Science & Technology SC Food Science & Technology GA CD8HT UT WOS:000351336500023 ER PT J AU Li, SX Darwish, O Alkharouf, N Matthews, B Ji, PS Domier, LL Zhang, N Bluhm, BH AF Li, Shuxian Darwish, Omar Alkharouf, Nadim Matthews, Benjamin Ji, Pingsheng Domier, Leslie L. Zhang, Ning Bluhm, Burton H. TI Draft genome sequence of Phomopsis longicolla isolate MSPL 10-6 SO GENOMICS DATA LA English DT Article DE Draft genome; Phomopsis longicolla; Phomopsis seed decay; Sequences; Soybean pathogen AB Phomopsis longicolla is the primary cause of Phomopsis seed decay in soybean. This disease severely affects soybean seed quality by reducing seed viability and oil content, altering seed composition, and increasing frequencies of moldy and/or split beans. It is one of the most economically important soybean diseases. Here, we report the de novo assembled draft genome sequence of the P. longicolla isolate MSPL10-6, which was isolated from field-grown soybean seed in Mississippi, USA. This study represents the first reported genome sequence of a seedborne fungal pathogen in the Diaporthe-Phomopsis complex. The P. longicolla genome sequence will enable research into the genetic basis of fungal infection of soybean seed and provide information for the study of soybean-fungal interactions. The genome sequence will also be valuable for molecular genetic marker development, manipulation of pathogenicity-related genes and development of new control strategies for this pathogen. Published by Elsevier Inc. C1 [Li, Shuxian] ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Darwish, Omar; Alkharouf, Nadim] Towson Univ, Dept Comp & Informat Sci, Towson, MD 21252 USA. [Matthews, Benjamin] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 21075 USA. [Ji, Pingsheng] Univ Georgia, Dept Plant Pathol, Tifton, GA 31794 USA. [Domier, Leslie L.] Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA. [Zhang, Ning] Rutgers State Univ, Dept Plant Biol Pathol, New Brunswick, NJ 08901 USA. [Zhang, Ning] Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08901 USA. [Bluhm, Burton H.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. RP Li, SX (reprint author), ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM shuxian.li@ars.usda.gov; bbluhm@uark.edu RI Zhang, Ning/K-3046-2012 OI Zhang, Ning/0000-0003-0755-2505 NR 8 TC 1 Z9 1 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2213-5960 J9 GENOM DATA JI Genom. Data PD MAR PY 2015 VL 3 BP 55 EP 56 DI 10.1016/j.gdata.2014.11.007 PG 2 WC Genetics & Heredity SC Genetics & Heredity GA CT2YY UT WOS:000362674200013 PM 26484148 ER PT J AU Degrandi-Hoffman, G Eckholm, B Huang, M AF Degrandi-Hoffman, Gloria Eckholm, Bruce Huang, Ming TI Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Molecular Biology; Issue 97; pollen; nutrition; microbes; protein; amino acids; Africanized bees; genotype; Apis mellifera; scutellata ID APIS-MELLIFERA; PRUNUS-DULCIS; POLLEN; MICROBIOLOGY; BREAD; BIOCHEMISTRY; EXPRESSION; INVASION; AMERICA; ALMOND AB Honey bees obtain nutrients from pollen they collect and store in the hive as beebread. We developed methods to control the pollen source that bees collect and convert to beebread by placing colonies in a specially constructed enclosed flight area. Methods were developed to analyze the protein and amino acid composition of the pollen and beebread. We also describe how consumption of the beebread was measured and methods used to determine adult worker bee hemolymph protein titers after feeding on beebread for 4, 7 and 11 days after emergence. Methods were applied to determine if genotype affects the conversion of pollen to beebread and the rate that bees consume and acquire protein from it. Two subspecies (European and Africanized honey bees; EHB and AHB respectively) were provided with the same pollen source. Based on the developed methods, beebread made by both subspecies had lower protein concentrations and pH values than the pollen. In general, amino acid concentrations in beebread made by either EHB or AHB were similar and occurred at higher levels in beebread than in pollen. Both AHB and EHB consumed significantly more of the beebread made by AHB than by EHB. Though EHB and AHB consumed similar amounts of each type of beebread, hemolymph protein concentrations in AHB were higher than in EHB. Differences in protein acquisition between AHB and EHB might reflect environmental adaptations related to the geographic region where each subspecies evolved. These differences could contribute to the successful establishment of AHB populations in the New World because of the effects on brood rearing and colony growth. C1 [Degrandi-Hoffman, Gloria] USDA ARS, Carl Hayden Bee Res Ctr, Washington, DC 20250 USA. [Huang, Ming] Eurofins Agrosci Serv Inc, Ecotoxicol Div, Sao Paulo, Brazil. RP Degrandi-Hoffman, G (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, Washington, DC 20250 USA. EM gloria.hoffman@ars.usda.gov OI Eckholm, Bruce/0000-0002-1661-925X NR 30 TC 0 Z9 0 U1 3 U2 8 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD MAR PY 2015 IS 97 AR e52448 DI 10.3791/52448 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR7MM UT WOS:000361534300028 ER PT J AU He, ZQ Chapital, DC AF He, Zhongqi Chapital, Dorselyn C. TI Preparation and Testing of Plant Seed Meal-based Wood Adhesives SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 97; Cottonseed meal; soy meal; oilseed; protein isolate; wood adhesive; water resistance; shear strength ID MODIFIED SOY PROTEIN; WATER RESISTANCE; PHYSICOCHEMICAL PROPERTIES; SODIUM BISULFITE; COTTONSEED MEAL; STRENGTH; IMPROVE; VENEER; COMPOSITES; LIGNIN AB Recently, the interest in plant seed meal-based products as wood adhesives has steadily increased, as these plant raw materials are considered renewable and environment-friendly. These natural products may serve as alternatives to petroleum-based adhesives to ease environmental and sustainability concerns. This work demonstrates the preparation and testing of the plant seed-based wood adhesives using cottonseed and soy meal as raw materials. In addition to untreated meals, water washed meals and protein isolates are prepared and tested. Adhesive slurries are prepared by mixing a freeze-dried meal product with deionized water (3: 25 w/w) for 2 hr. Each adhesive preparation is applied to one end of 2 wood veneer strips using a brush. The tacky adhesive coated areas of the wood veneer strips are lapped and glued by hot-pressing. Adhesive strength is reported as the shear strength of the bonded wood specimen at break. Water resistance of the adhesives is measured by the change in shear strength of the bonded wood specimens at break after water soaking. This protocol allows one to assess plant seed-based agricultural products as suitable candidates for substitution of synthetic-based wood adhesives. Adjustments to the adhesive formulation with or without additives and bonding conditions could optimize their adhesive properties for various practical applications. C1 [He, Zhongqi; Chapital, Dorselyn C.] ARS, So Reg Res Ctr, USDA, New York, NY 10007 USA. RP He, ZQ (reprint author), ARS, So Reg Res Ctr, USDA, New York, NY 10007 USA. EM Zhongqi.He@ars.usda.gov FU Agency's in-house funding FX Data reported in this work are part of the USDA-ARS National Program 306 Project 'Values-Added Products from Cottonseed' research supported by the Agency's in-house funding. Publication of this paper is supported in part by the Journal of Visualized Experiments. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. We acknowledge the constructive comments from JoVE science editor and peer reviewers in the review and revision process. NR 29 TC 0 Z9 0 U1 1 U2 2 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD MAR PY 2015 IS 97 AR e52557 DI 10.3791/52557 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR7MM UT WOS:000361534300046 ER PT J AU Kowalski, RJ Morris, CF Ganjyal, GM AF Kowalski, Ryan J. Morris, Craig F. Ganjyal, Girish M. TI Waxy Soft White Wheat: Extrusion Characteristics and Thermal and Rheological Properties SO CEREAL CHEMISTRY LA English DT Article ID END-USE QUALITY; AMYLOSE CONTENT; STARCH GELATINIZATION; EXTRUDED CORN; RICE FLOUR; EXPANSION; COOKING; PRODUCTS; GRITS; RETROGRADATION AB Waxy wheat flour was analyzed for its thermal and rheological properties and was extruded to evaluate its potential for extruded products. Normal soft white wheat flour was analyzed with the same methods and same extrusion conditions to directly compare differences between the two types of flour. Through DSC analysis, waxy wheat flour was found to have a higher gelatinization peak temperature of 66.4 degrees C than normal wheat at 64.0 degrees C, although the transition required 2.00 J/g less energy. Rapid visco-analysis indicated that the waxy wheat flour pasted much more quickly and at lower temperatures than the normal wheat flour. Preliminary extrusion experiments were conducted to determine the optimal screw profile for waxy wheat with respect to maximum radial expansion. The optimum screw profile was used for extrusion trials with varying flour moisture (15-25% wb) and extruder screw speed (200-400 rpm) while monitoring process conditions including back pressure and specific mechanical energy. Physical properties of the extrudates were then studied. The radial expansion ratios of the waxy wheat extrudates exceeded those of the normal wheat extrudates by nearly twice as much, and it was observed that the waxy wheat flour took less energy in the form of fewer shear screw elements to expand. The waxy wheat extrudates also exhibited significantly higher water solubility and less water absorption than the normal wheat extrudates owing to solubilizing of the extrudates. The results of our study indicate that waxy wheat flour may be a viable ingredient for creating direct expanded products with less energy. C1 [Kowalski, Ryan J.; Ganjyal, Girish M.] Washington State Univ, Sch Food Sci, Pullman, WA 99163 USA. [Morris, Craig F.] USDA ARS, Western Wheat Qual Lab, Pullman, WA 99163 USA. RP Ganjyal, GM (reprint author), Washington State Univ, Sch Food Sci, Pullman, WA 99163 USA. EM girish.ganjyal@wsu.edu NR 46 TC 4 Z9 4 U1 4 U2 11 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2015 VL 92 IS 2 BP 145 EP 153 DI 10.1094/CCHEM-03-14-0039-R PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK3CO UT WOS:000356093700003 ER PT J AU Delwiche, SR Vinyard, BT Bettge, AD AF Delwiche, Stephen R. Vinyard, Bryan T. Bettge, Arthur D. TI Repeatability Precision of the Falling Number Procedure Under Standard and Modified Methodologies SO CEREAL CHEMISTRY LA English DT Article ID ALPHA-AMYLASE ACTIVITY; WHEAT-STARCH; HYDROLYSIS AB The falling number (FN) procedure is used worldwide to assess the integrity of the starch stored within wheat seed. As an indirect measurement of the activity level of alpha-amylase, FN relies on a dedicated viscometer that measures the amount of time needed for a metal stirring rod of precise geometry to descend a fixed distance through a column of water-flour or water-meal slurry that undergoes enzyme-activated starch hydrolysis under controlled mixing and heating conditions. For U.S. wheat, FN values of 300 s and above generally indicate soundness in the condition of the seed starches, whereas values less than 300 s often indicate that some seeds have broken dormancy, which deleteriously affects bread-, cake-, and noodle-making quality of products derived from their flour. Domestic and especially overseas sales contracts will often specify a minimum FN value for consignments, thus making it critical to ensure that the FN procedure be highly precise. The study described herein examined the level of repeatability precision of the FN procedure under strictly controlled laboratory conditions as a means to establish precision levels arising alone from the random nature of the viscous properties of starchy meal undergoing mixing and heating. Six representative samples of Pacific Northwest-grown soft white and club wheat, ranging in FN between 168 and 404 s, were repeatedly measured with the conventional FN procedure and three modifications thereof, with the modifications being increased meal and water amounts (8 g of meal + 30 mL of H2O instead of 7 g + 25 mL) or the addition of a polysorbate surfactant (0.1% Tween 20) to the mixture water. Based on 16 FN runs for each sample and treatment, estimated variances and coefficients of variation (CV) were determined for each treatment-sample combination. The results indicated that CVs between 1 and 4% were achieved for all treatments and samples. The treatment modification of an augmented test sample size improved precision, whereas incorporating a surfactant had a negligible effect. Precision and treatment (conventional versus augmented) findings were corroborated by two external laboratories with two of the six original samples and by the main laboratory on an independent set of 14 wheat meal samples of commercial origin. Another possible improvement in precision is in the sequencing of water and meal addition to be half the volume of water, then the meal, followed by the remaining volume of water, all at the conventional levels of meal and water. Preliminary experimental results indicate an improvement in precision by using the "sandwich" approach; however, further testing is warranted to substantiate this discovery. C1 [Delwiche, Stephen R.] USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. [Vinyard, Bryan T.] USDA ARS, Beltsville Agr Res Ctr, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Bettge, Arthur D.] ADB Wheat Consulting, Moscow, ID USA. [Bettge, Arthur D.] USDA ARS, Washington, DC 20250 USA. RP Delwiche, SR (reprint author), USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Bldg 303,BARC East, Beltsville, MD 20705 USA. EM stephen.delwiche@ars.usda.gov NR 16 TC 0 Z9 0 U1 5 U2 7 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2015 VL 92 IS 2 BP 177 EP 184 DI 10.1094/CCHEM-07-14-0156-R PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK3CO UT WOS:000356093700007 ER PT J AU Ramchandran, D Wang, P Dien, B Liu, W Cotta, MA Singh, V AF Ramchandran, Divya Wang, Ping Dien, Bruce Liu, Wei Cotta, Michael A. Singh, Vijay TI Improvement of Dry-Fractionation Ethanol Fermentation by Partial Germ Supplementation SO CEREAL CHEMISTRY LA English DT Article ID STARCH HYDROLYZING ENZYME; GRIND CORN PROCESSES AB Ethanol fermentation of dry-fractionated grits (corn endosperm pieces) containing different levels of germ was studied with the dry-grind process. Partial removal of the germ fraction allows for marketing the germ fraction and potentially more efficient fermentation. Grits obtained from a dry-milling plant were mixed with different amounts of germ (2, 5, 7, and 10% germ of the total sample) and compared with control grits (0% germ). Fermentation rates of germ-supplemented grits (2, 5, 7, and 10% germ) were faster than control grits (0% germ). Addition of 2% germ was sufficient to achieve a high ethanol concentration (19.06% v/v) compared with control grits (18.18% v/v). Fermentation of dry-fractionated grits (92, 95, and 97% grits) obtained from a commercial facility was also compared with ground whole corn (control). Fermentation rates were slower and final ethanol concentrations were lower for commercial grits than the control sample. However, in a final experiment, commercial grits were subjected to raw starch hydrolyzing (RSH) enzyme, resulting in higher ethanol concentrations (20.22, 19.90, and 19.49% v/v for 92, 95, and 97% grits, respectively) compared with the whole corn control (18.64% v/v). Therefore, high ethanol concentrations can be achieved with dry-fractionated grits provided the inclusion of a certain amount of germ and the use of RSH enzyme for controlled starch hydrolysis. C1 [Ramchandran, Divya; Liu, Wei; Singh, Vijay] Univ Illinois, Agr & Biol Engn, Urbana, IL 61801 USA. [Wang, Ping] US DOE, Natl Energy Technol Lab, Pittsburgh, PA 15236 USA. [Dien, Bruce; Cotta, Michael A.] USDA, Natl Ctr Agr Utilizat Res, Agr Res Serv, Peoria, IL 61604 USA. RP Singh, V (reprint author), Univ Illinois, Agr & Biol Engn, 1304 Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu OI Dien, Bruce/0000-0003-3863-6664 FU Gevo, Inc. FX We thank Patricia O'Bryan for her excellent technical help in conducting fermentations. We are grateful to Jim Hettenhaus and Robert Wooley for organizing this study and their valuable guidance. We also thank Gevo, Inc., for their partial financial support. NR 12 TC 0 Z9 0 U1 0 U2 1 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2015 VL 92 IS 2 BP 218 EP 223 DI 10.1094/CCHEM-08-14-0177-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK3CO UT WOS:000356093700013 ER PT J AU Fu, SM Hartung, J Zhou, CY Su, HN Tan, J Li, ZA AF Fu, S. M. Hartung, John Zhou, C. Y. Su, H. N. Tan, J. Li, Z. A. TI Ultrastructural Changes and Putative Phage Particles Observed in Sweet Orange Leaves Infected with 'Candidatus Liberibacter asiaticus' SO PLANT DISEASE LA English DT Article ID COMPLETE GENOME SEQUENCE; CA. L. AMERICANUS; CALCIUM-OXALATE; TANDEM REPEATS; CITRUS; DISEASE; PLANTS; GUANGDONG; SYMPTOMS; BRAZIL AB Huanglongbing (HLB), also known as citrus greening, is currently the most destructive citrus disease. Anatomical analyses of HLB-affected sweet orange were carried out by light and electron microscopy. As compared with healthy citrus, the phloem plasmodesmata were plugged with callose, and in some samples the phloem was collapsed. Chloroplast structures were deformed. Prophage sequences occupy a significant portion of the genome of 'Candidatus Liberibacter asiaticus' and have been used to distinguish strains from Yunnan and Guangdong provinces in China and Florida. Interestingly, a large number of possible putative phage particles were observed attached on the surface of 'Ca. L. asiaticus' cells in plants inoculated with strain FJ3 from Fujian Province, China. Phage particles have been observed previously only in periwinkle plants artificially inoculated in Florida with 'Ca. L. asiaticus' that carried the SC1-type prophage. PCR assays verified the presence of the SC1-type prophage sequences previously described from this bacterium in Florida in the FJ3 isolate. This is the first time that suspected phage particles have been observed in sweet orange trees infected with 'Ca. L. asiaticus.' C1 [Fu, S. M.] Southwest Univ, Citrus Res Inst, Coll Plant Protect, Chongqing 400715, Peoples R China. [Fu, S. M.; Hartung, John] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Zhou, C. Y.; Su, H. N.; Tan, J.; Li, Z. A.] Southwest Univ, Citrus Res Inst, Chongqing 400715, Peoples R China. RP Hartung, J (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM john.hartung@ars.usda.gov FU MOA's Public Benefit Research Foundation of China [201203076, 200903004-06] FX This research was supported by MOA's Public Benefit Research Foundation of China (201203076, 200903004-06). We thank Prof. J. Hong and his team for technical assistance on electron microscopy at Zhejiang University. NR 32 TC 1 Z9 1 U1 2 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 320 EP 324 DI 10.1094/PDIS-01-14-0106-RE PG 5 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500003 ER PT J AU Stulberg, MJ Shao, J Huang, Q AF Stulberg, Michael J. Shao, Jonathan Huang, Qi TI A Multiplex PCR Assay to Detect and Differentiate Select Agent Strains of Ralstonia solanacearum SO PLANT DISEASE LA English DT Article ID PSEUDOMONAS-SOLANACEARUM; REAL-TIME; BROWN-ROT; BIOVAR 2; DIVERSITY; PLANT; GERANIUM; POTATO; PHYLOGENY; FLORIDA AB Ralstonia solanacearum race 3 biovar 2 strains are considered select agents by the U.S. government because they are not endemic to the United States and have the potential to cause brown rot in our potato production fields. Simple and accurate methods are needed for quick identification prior to more discriminating but time-consuming verification methods. We developed a multiplex PCR assay that identifies R. solanacearum species complex strains, signals whether the strain detected is a select agent, and controls for false negatives associated with PCR inhibition or unsuccessful DNA extractions in one reaction. We identified unique sequences of non-phage-related DNA for the R. solanacearum species complex strains, and for select agent strains, using in silico genome subtraction. We also designed and included an internal plant DNA control assay. Our multiplex PCR assay correctly identified 90 R. solanacearum species complex strains and 34 select agent strains, while not recognizing five out-group bacterial species. Additionally, the multiplex PCR assay facilitated the detection of plant DNA and R. solanacearum from infected tomato, potato, geranium, and tobacco plants. Our rapid, accurate, and reliable detection assay can help government officials make timely and appropriate recommendations to exclude this bacterium from the United States. C1 [Stulberg, Michael J.; Huang, Qi] USDA ARS, US Natl Arboretum, Floral & Nursery Plant Res Unit, Beltsville, MD 20705 USA. [Shao, Jonathan] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. RP Huang, Q (reprint author), USDA ARS, US Natl Arboretum, Floral & Nursery Plant Res Unit, Beltsville, MD 20705 USA. EM qi.huang@ars.usda.gov FU U. S. Department of Agriculture FX This research was supported by the U. S. Department of Agriculture. We thank P. Prior in France, M. M. Lopez in Spain, J. F. Wang in Taiwan, J. H. Gao in China, C. Allen, J. Jones, and D. J. Norman in the United States for providing R. solanacearum strains, as well as J. Hartung in the United States for providing other bacterial strains for our study. NR 37 TC 5 Z9 5 U1 1 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 333 EP 341 DI 10.1094/PDIS-05-14-0483-RE PG 9 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500005 ER PT J AU Rosenzweig, N Hanson, LE Clark, G Franc, GD Stump, WL Jiang, QW Stewart, J Kirk, WW AF Rosenzweig, N. Hanson, L. E. Clark, G. Franc, G. D. Stump, W. L. Jiang, Q. W. Stewart, J. Kirk, W. W. TI Use of PCR-RFLP Analysis to Monitor Fungicide Resistance in Cercospora beticola Populations from Sugarbeet (Beta vulgaris) in Michigan, United States SO PLANT DISEASE LA English DT Article ID F-SP TRITICI; TUBULIN GENE; BENZIMIDAZOLE RESISTANCE; VENTURIA-INAEQUALIS; STROBILURIN FUNGICIDES; BENOMYL-RESISTANCE; BOTRYTIS-CINEREA; FIELD STRAINS; LEAF-SPOT; CONFERRING RESISTANCE AB Genetic resistance to Quinone outside inhibitor (QoI) and benzimidazole fungicides may be responsible for a recent decline in efficacy of chemical control management strategies for Cercospora leaf spot (CLS) caused by Cercospora beticola in Michigan sugarbeet (Beta vulgaris) fields. The target genes and fungicide resistance mutations are known for these two fungicides. Based on this, two standard polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays were developed to detect the G143A and E198A point mutations in the fungal mitochondrial cytochrome b and the beta-tubulin genes, respectively. These mutations confer a high level of resistance to either QoI or benzimidazole fungicides. The presence of the G143A and E198A mutations was monitored within C. beticola populations recovered from Michigan sugarbeet production fields collected in 2012. Both the QoI-resistant cytochrome b allele and the benzimidazole-resistant beta-tubulin allele were detected directly from leaf tissue following a PCR-RFLP assay. Using either detection assay, the G143A and E198A mutations were detected in over 90% of the 118 field samples originating from Michigan sugarbeet production under fungicide management programs for CLS control. Monitoring of the G143A and E198A mutations in fields located in 9 counties and 58 townships indicated that the mutations were widespread in Michigan sugarbeet production areas. The PCR-based assays used and developed in this study were effective in detecting the presence of the G143A and E198A mutations in C. beticola field populations from Michigan. C1 [Rosenzweig, N.; Jiang, Q. W.; Kirk, W. W.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Hanson, L. E.] USDA ARS, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Clark, G.; Stewart, J.] Michigan Sugar Co, Bay City, MI 48706 USA. [Franc, G. D.; Stump, W. L.] Univ Wyoming, Dept Plant Sci, Laramie, WY 82071 USA. RP Kirk, WW (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 612,Wilson Rd,35 Plant Biol Bldg, E Lansing, MI 48824 USA. EM kirkw@msu.edu FU Michigan Sugar Company Research & Education Advisory Council; Michigan State University Project GREEEN (Generating Research and Extension to Meet Economic and Environmental Needs); CSREES Hatch Project [MICL01966] FX In memory of Gary D. Franc, who died too soon, October 2012. This project was partially supported by the Michigan Sugar Company Research & Education Advisory Council and the Michigan State University Project GREEEN (Generating Research and Extension to Meet Economic and Environmental Needs) and CSREES Hatch Project Number MICL01966. The authors thank all the agriculturists from Michigan Sugar Company that assisted in collection of samples. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement. NR 50 TC 2 Z9 2 U1 4 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 355 EP 362 DI 10.1094/PDIS-03-14-0241-RE PG 8 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500008 ER PT J AU Moore, MJ Ostry, ME Hegeman, AD Martin, AC AF Moore, M. J. Ostry, M. E. Hegeman, A. D. Martin, A. C. TI Inhibition of Ophiognomonia clavigignenti-juglandacearum by Juglans Species Bark Extracts SO PLANT DISEASE LA English DT Article ID FUSICLADIUM-EFFUSUM; PHENOLIC-ACIDS; WALNUT; JUGLONE; RESISTANCE; TREES; NAPHTHOQUINONES; BUTTERNUT; GROWTH; REGIA AB A rapid and reliable technique is needed for identifying butternut trees (Juglans cinerea) with resistance to butternut canker. We investigated the potential of a bark extract bioassay to detect levels of resistance to Ophiognomonia clavigignenti-juglandacearum (Oc-j), the causal agent of butternut canker. Both reagent grade naphthoquinones and crude bark extracts of Juglans species inhibited germination of Oc-j conidia. A disc diffusion bioassay was used to study the level of inhibition by these bark extracts and results indicated extensive variation within and between butternut and other species of Juglans tested. In many months over a 3 year period, bark from butternut trees selected for apparent disease resistance could be distinguished from that of unselected trees. Inhibition of conidia germination roughly correlated to the level of resistance observed in field inoculations of the trees. Quantification of the naphthoquinone compounds juglone and plumbagin in butternut bark was performed using ultra-high performance liquid chromatography mass spectrometry. While the concentrations of these two compounds varied by month and by individual tree, juglone levels correlated well with the bark extract bioassay in some months. These results suggest that juglone concentration may account in part for the observed range of inhibition observed in the bioassay and variation in canker resistance among selections of butternut field inoculated with Oc-j. The bark extract bioassay described in the following report may have potential use for selecting resistant butternut for conservation and restoration purposes. C1 [Moore, M. J.; Ostry, M. E.] USDA Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Hegeman, A. D.; Martin, A. C.] Univ Minnesota, Dept Hort, St Paul, MN 55108 USA. RP Moore, MJ (reprint author), USDA Forest Serv, No Res Stn, St Paul, MN 55108 USA. EM melaniemoore@fs.fed.us OI Hegeman, Adrian/0000-0003-1008-6066 NR 33 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 401 EP 408 DI 10.1094/PDIS-06-14-0642-RE PG 8 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500014 ER PT J AU Bull, CT Ortiz-Lytle, MC Ibarra, AG du Toit, LJ Reynolds, G AF Bull, C. T. Ortiz-Lytle, M. C. Ibarra, A. G. du Toit, L. J. Reynolds, G. TI First Report of Bacterial Blight of Crucifers Caused by Pseudomonas cannabina pv. alisalensis in Minnesota on Arugula (Eruca vesicaria subsp sativa). SO PLANT DISEASE LA English DT News Item C1 [Bull, C. T.] USDA ARS, Agr Res Stn, Salinas, CA 93905 USA. [Ortiz-Lytle, M. C.] Calif State Univ, Undergrad Res Opportun Ctr, Seaside, CA 93955 USA. [Ibarra, A. G.] Hartnell Coll, Sci & Math Inst, Salinas, CA 93901 USA. [du Toit, L. J.] Washington State Univ Mt Vernon NWREC, Mt Vernon, WA 98273 USA. [Reynolds, G.] Riverbend Farm, Delano, MN 55328 USA. RP Bull, CT (reprint author), USDA ARS, Agr Res Stn, Salinas, CA 93905 USA. NR 2 TC 1 Z9 1 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 415 EP 416 DI 10.1094/PDIS-10-14-1020-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500018 ER PT J AU Murillo-Williams, A Esker, P Allen, T Stone, C Frederick, R AF Murillo-Williams, A. Esker, P. Allen, T. Stone, C. Frederick, R. TI First Report of Phakopsora pachyrhizi on Soybean in Costa Rica. SO PLANT DISEASE LA English DT News Item C1 [Murillo-Williams, A.] Univ Costa Rica, Ctr Invest Granos & Semillas, San Pedro Montes De Oca, Costa Rica. [Esker, P.] Univ Costa Rica, Ctr Invest Protecc Cultivos, San Pedro Montes De Oca, Costa Rica. [Allen, T.] Univ Mississippi, Dept Biochem Mol Biol Entomol & Plant Pathol, Stoneville, MS USA. [Stone, C.; Frederick, R.] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA. RP Murillo-Williams, A (reprint author), Univ Costa Rica, Ctr Invest Granos & Semillas, San Pedro Montes De Oca, Costa Rica. NR 2 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 418 EP 418 DI 10.1094/PDIS-06-14-0646-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500025 ER PT J AU Murithi, HM Beed, FD Soko, MM Haudenshield, JS Hartman, GL AF Murithi, H. M. Beed, F. D. Soko, M. M. Haudenshield, J. S. Hartman, G. L. TI First Report of Phakopsora pachyrhizi Causing Rust on Soybean in Malawi. SO PLANT DISEASE LA English DT News Item C1 [Murithi, H. M.; Beed, F. D.] Int Inst Trop Agr, Dar Es Salaam, Tanzania. [Soko, M. M.] Bvumbwe Agr Res Stn, Bvumbwe, Malawi. [Haudenshield, J. S.; Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Haudenshield, J. S.; Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Murithi, HM (reprint author), Int Inst Trop Agr, Dar Es Salaam, Tanzania. OI Harun, Murithi/0000-0001-7455-1188 NR 4 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 420 EP 420 DI 10.1094/PDIS-09-14-0924-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500030 ER PT J AU Gauthier, NW Polashock, J Veetil, TT Mar-Tin, RR Beale, J AF Gauthier, N. W. Polashock, J. Veetil, T. T. Mar-Tin, R. R. Beale, J. TI First Report of Blueberry Mosaic Disease Caused by Blueberry mosaic associated virus in Kentucky. SO PLANT DISEASE LA English DT News Item C1 [Gauthier, N. W.; Beale, J.] Univ Kentucky, Lexington, KY 40546 USA. [Polashock, J.] USDA ARS, Chatsworth, NJ 08019 USA. [Veetil, T. T.] Univ Arkansas, Fayetteville, AR 72701 USA. [Mar-Tin, R. R.] USDA ARS, Corvallis, OR 97330 USA. RP Gauthier, NW (reprint author), Univ Kentucky, Lexington, KY 40546 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2015 VL 99 IS 3 BP 421 EP 422 DI 10.1094/PDIS-09-14-0946-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CQ8NR UT WOS:000360865500034 ER PT J AU Allan, E Manter, D Jung, G AF Allan, E. Manter, D. Jung, G. TI Comparison of nematode communities between organically and conventionally managed golf courses SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Allan, E.; Jung, G.] Univ Massachusetts, Amherst, MA 01003 USA. [Manter, D.] USDA ARS SPNR, Ft Collins, CO USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500019 ER PT J AU Chavez, JDC Vargas, LIR Segarra, AE Davis, RE AF Chavez, J. D. Caicedo Vargas, L. I. Rivera Segarra, A. E. Davis, R. E. TI Identification and molecular characterization of pigeon pea witches'-broom phytoplasma in plants and its potential vectors in Puerto Rico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Chavez, J. D. Caicedo; Vargas, L. I. Rivera; Segarra, A. E.] Univ Puerto Rico, Coll Agr Sci, Agroenvironm Sci, Mayaguez, PR USA. [Davis, R. E.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 2 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500004 ER PT J AU Davis, RE Zhao, Y Lee, IM Wei, W AF Davis, R. E. Zhao, Y. Lee, I. M. Wei, W. TI Evaluation of Phaeoisariopsis griseola on common bean Phaseolus vulgaris in Puerto Rico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Davis, R. E.; Zhao, Y.; Lee, I. M.; Wei, W.] USDA ARS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500006 ER PT J AU Davis, RE Zhao, Y Lee, IM Wei, W AF Davis, R. E. Zhao, Y. Lee, I. M. Wei, W. TI 3-dimensional modeling of protein structures distinguishes closely related phytoplasmas SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Davis, R. E.; Zhao, Y.; Lee, I. M.; Wei, W.] USDA ARS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500005 ER PT J AU Hartung, JS Roy, A Shao, J Schneider, W Brlansky, RH AF Hartung, J. S. Roy, A. Shao, J. Schneider, W. Brlansky, R. H. TI History of Citrus leprosis virus recorded in herbarium specimens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Hartung, J. S.; Shao, J.] USDA ARS, Beltsville, MD USA. [Roy, A.; Schneider, W.] USDA ARS, Frederick, MD USA. [Brlansky, R. H.] Univ Florida, Lake Alfred, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500010 ER PT J AU Polashock, J AF Polashock, J. TI The causal agent(s) of blueberry stem blight disease in New Jersey SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Polashock, J.] USDA ARS, Chatsworth, NJ USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500033 ER PT J AU Serrato-Diaz, LM Rivera-Vargas, LI Goenaga, R French-Monar, RD AF Serrato-Diaz, L. M. Rivera-Vargas, L. I. Goenaga, R. French-Monar, R. D. TI Fungal pathogen complexes associated with diseases of rambutan, longan and mango in Puerto Rico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Serrato-Diaz, L. M.] Univ Puerto Rico, Dept Biol, Rio Piedras, PR 00931 USA. [Rivera-Vargas, L. I.] Univ Puerto Rico, Dept Crops & Agroenvironm Sci, Mayaguez, PR USA. [Goenaga, R.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR USA. [French-Monar, R. D.] Texas A&M AgriLife Extens Serv, Dept Plant Pathol & Microbiol, Amarillo, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500015 ER PT J AU Vargas, A Porch, T Beaver, J AF Vargas, A. Porch, T. Beaver, J. TI Evaluation of the tepary bean (Phaseolus acutifolius) CIAT germplasm collection for response to Bean Common Mosaic Necrosis Virus (BCMNV) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the Caribbean-Division of American-Phytopathological-Society (APS) and the Caribbean-Food-Crop-Society (CFCS) CY JUL 07-09, 2014 CL VI SP Amer Phytopathol Soc, Caribbean Div, Caribbean Food Crop Soc C1 [Vargas, A.; Beaver, J.] Univ Puerto Rico, Mayaguez, PR USA. [Porch, T.] USDA ARS TARS, Mayaguez, PR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 SU 1 PG 1 WC Plant Sciences SC Plant Sciences GA CP0VP UT WOS:000359594500017 ER PT J AU Rasooly, R Hernlem, B He, XH Friedman, M AF Rasooly, Reuven Hernlem, Bradley He, Xiaohua Friedman, Mendel TI Plant Compounds Enhance the Assay Sensitivity for Detection of Active Bacillus cereus Toxin SO TOXINS LA English DT Article DE food poisoning; Bacillus cereus bacteria; inactivation; Bacillus cereus toxin; enterotoxins; plant compounds; plant extracts; cell based assay; food safety ID ANTIMICROBIAL RESISTANCE; ANTIBIOTIC-RESISTANCE; ESSENTIAL OILS; PRODUCTS; GROWTH; PREVALENCE; CARVACROL; FOODS; RICE; MILK AB Bacillus cereus is an important food pathogen, producing emetic and diarrheal syndromes, the latter mediated by enterotoxins. The ability to sensitively trace and identify this active toxin is important for food safety. This study evaluated a nonradioactive, sensitive, in vitro cell-based assay, based on B. cereus toxin inhibition of green fluorescent protein (GFP) synthesis in transduced monkey kidney Vero cells, combined with plant extracts or plant compounds that reduce viable count of B. cereus in food. The assay exhibited a dose dependent GFP inhibition response with similar to 25% inhibition at 50 ng/mL toxin evaluated in culture media or soy milk, rice milk or infant formula, products associated with food poisonings outbreak. The plant extracts of green tea or bitter almond and the plant compounds epicatechin or carvacrol were found to amplify the assay response to similar to 90% inhibition at the 50 ng/mL toxin concentration greatly increasing the sensitivity of this assay. Additional studies showed that the test formulations also inhibited the growth of the B. cereus bacteria, likely through cell membrane disruption. The results suggest that the improved highly sensitive assay for the toxin and the rapid inactivation of the pathogen producing the toxin have the potential to enhance food safety. C1 [Rasooly, Reuven; Hernlem, Bradley; He, Xiaohua] ARS, Foodborne Contaminants Res Unit, USDA, Albany, CA 94710 USA. [Friedman, Mendel] ARS, Healthy Processed Foods Res Unit, USDA, Albany, CA 94710 USA. RP Rasooly, R (reprint author), ARS, Foodborne Contaminants Res Unit, USDA, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov; bradley.hernlem@ars.usda.gov; xiaohua.he@ars.usda.gov; mendel.friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 NR 26 TC 1 Z9 1 U1 2 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD MAR PY 2015 VL 7 IS 3 BP 835 EP 845 DI 10.3390/toxins7030835 PG 11 WC Toxicology SC Toxicology GA CO7LB UT WOS:000359339500014 PM 25767986 ER PT J AU Zong, X Wang, W Wei, H Wang, J Yan, X Hammond, RW Liu, Q AF Zong, X. Wang, W. Wei, H. Wang, J. Yan, X. Hammond, R. W. Liu, Q. TI INCIDENCE OF SWEET CHERRY VIRUSES IN SHANDONG PROVINCE, CHINA AND A CASE STUDY ON MULTIPLE INFECTION WITH FIVE VIRUSES SO JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Prunus necrotic ringspot virus; Prune dwarf virus; Little cherry virus-2; Cherry virus A; Cherry green ring mottle virus; sweet cherry; RT-PCR; phylogenetic analysis of Ilarviruses ID NECROTIC-RINGSPOT-VIRUS; RUSTY-MOTTLE-VIRUS; 1ST REPORT; NUCLEOTIDE-SEQUENCE; FRUIT-TREES; ILARVIRUSES; VARIABILITY AB Leaves were collected from a total of 62 trees displaying virus-like disease symptoms such as rugose mosaic in leaf, irregular shaped leaf blades or small leaf blades in a survey of sweet cherry (Prunus avium L. cv Red Lamp) in Shandong Province, the largest cherry production area in China. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Little cherry virus-2 (LChV-2), Cherry virus A (CVA) and Cherry green ring mottle virus (CGRMV) were present and that most of the samples (70%) were infected by at least two viruses. A case study in this survey of two selected trees revealed that a mixed infection with these five viruses simultaneously caused two types of field symptoms: late-maturity in fruits or fruit blast. Real-time quantitative RT-PCR analysis revealed that the relative proportion of the virus titers was distinct in the two sampled trees. The PNRSV titer was significantly higher than the four other viruses in the trees that exhibited fruit blast. Phylogenetic analysis of the PNRSV coat protein (CP) gene showed that the isolates HSY-4-1 and X-3, obtained from the two sampled trees separately, were classified into different subgroups of Group I isolates, which is characterized by a six-nucleotide insertion. A non-synonymous substitution was identified in the six-nucleotide insertion in the PNRSV CP gene in one of the two isolates. This is the first detailed study of the incidence of multiple sweet cherry viruses in China. C1 [Zong, X.; Wang, W.; Wei, H.; Wang, J.; Liu, Q.] Shandong Acad Agr Sci, Shandong Inst Pomol, Key Lab Fruit Tree Biotechnol Breeding Shandong P, Tai An 271000, Shandong, Peoples R China. [Hammond, R. W.] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Yan, X.] Shenyang Agr Univ, Coll Plant Protect, Shenyang 110866, Liaoning, Peoples R China. RP Liu, Q (reprint author), Shandong Acad Agr Sci, Shandong Inst Pomol, Key Lab Fruit Tree Biotechnol Breeding Shandong P, Tai An 271000, Shandong, Peoples R China. EM rose.hammond@ars.usda.gov; qzliu@sdip.cn FU Program for International S&T Cooperation Project of China [2012DFR30700]; Special Fund for Agro-scientific Research in the Public Interest of China [200903019]; Agricultural Breeding Project of Shandong Province, China FX The authors are grateful to Dr. Yan Zhao and Dr. John Hammond for their useful suggestions and comments. This work was supported by Program for International S&T Cooperation Project of China (2012DFR30700), Special Fund for Agro-scientific Research in the Public Interest of China (200903019) and Agricultural Breeding Project of Shandong Province, China. NR 22 TC 1 Z9 1 U1 0 U2 3 PU EDIZIONI ETS PI PISA PA PIAZZA CARRARA 16-19, 56126 PISA, ITALY SN 1125-4653 J9 J PLANT PATHOL JI J. Plant Pathol. PD MAR PY 2015 VL 97 IS 1 BP 61 EP 68 PG 8 WC Plant Sciences SC Plant Sciences GA CN9TY UT WOS:000358793400006 ER PT J AU Romanov, D Divashuk, M Havey, MJ Khrustaleva, L AF Romanov, Dmitry Divashuk, Mikhail Havey, Michael J. Khrustaleva, Ludmila TI Tyramide-FISH mapping of single genes for development of an integrated recombination and cytogenetic map of chromosome 5 of Allium cepa SO GENOME LA English DT Article DE Allium cepa; Tyramide-FISH; chromosome; EST; recombination ID IN-SITU HYBRIDIZATION; ULTRA-SENSITIVE FISH; SIGNAL AMPLIFICATION; EXPRESSED SEQUENCE; MULTICOLOR FISH; GENOME ANALYSIS; PHYSICAL MAPS; RICH REGIONS; BULB ONION; MAIZE AB Chromosome 5 of onion carries major quantitative trait loci (QTL) that control dry-matter content, pungency and storability of bulbs, amounts and types of epicuticular waxes, and resistances to abiotic factors, all of which are of interest to breeders. SNPs, SSRs, and RFLPs in expressed regions of the onion genome have been genetically mapped, and we used these clones and sequences from the NCBI database to develop DNA probes for in situ hybridization to integrate the genetic and physical maps of onion chromosome 5. We produced genomic amplicons from expressed regions of the onion genome that carried both exons and introns in order to increase the hybridization specificity of the probes and to enlarge the target DNA sizes. Tyramide-FISH technique was used to increase the detection sensitivity of relatively short target DNA regions, which range from 950 to 2100 bp. Through the integration of genetic and chromosomal maps, we were able to estimate the distribution of recombination events along onion chromosome 5. We demonstrated the efficiency of chromosomal in situ mapping of exon-intron genomic clones for the extremely large genome of onion. C1 [Romanov, Dmitry; Divashuk, Mikhail; Khrustaleva, Ludmila] Russian State Agr Univ MTAA, Ctr Mol Biotechnol, Moscow 127550, Russia. [Havey, Michael J.] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA. [Havey, Michael J.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. RP Khrustaleva, L (reprint author), Russian State Agr Univ MTAA, Ctr Mol Biotechnol, Moscow 127550, Russia. EM ludmila.khrustaleva19@gmail.com OI Divashuk, Mikhail/0000-0001-6221-3659 FU Russian Foundation for Basic Research [14-04-31595 mol_a]; Ministry of Education and Science of Russian Federation [GK P809] FX The reported study was supported partly by the Russian Foundation for Basic Research (research project No. 14-04-31595 mol_a) and partly by the Ministry of Education and Science of Russian Federation (Project GK P809). We thank Anna Kiseleva and Ilya Kirov for technical assistance. NR 51 TC 2 Z9 2 U1 2 U2 7 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD MAR PY 2015 VL 58 IS 3 BP 111 EP 119 DI 10.1139/gen-2015-0019 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CM5KH UT WOS:000357726200003 PM 26158384 ER PT J AU Keele, JW Kuehn, LA McDaneld, TG Tait, RG Jones, SA Smith, TPL Shackelford, SD King, DA Wheeler, TL Lindholm-Perry, AK McNeel, AK AF Keele, J. W. Kuehn, L. A. McDaneld, T. G. Tait, R. G., Jr. Jones, S. A. Smith, T. P. L. Shackelford, S. D. King, D. A. Wheeler, T. L. Lindholm-Perry, A. K. McNeel, A. K. TI Genomewide association study of lung lesions in cattle using sample pooling SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; bovine respiratory disease complex; disease resistance; genomewide association study; lung lesions; pneumonia ID BOVINE RESPIRATORY-DISEASE; FEEDLOT CATTLE; TREATMENT RECORDS; BEEF-CATTLE; POLYMORPHISMS; PERFORMANCE; SLAUGHTER; TRAITS; SCORES; IMPACT AB Bovine respiratory disease complex (BRDC) is the most expensive disease in beef cattle in the United States costing the industry at least US$1 billion annually. Bovine respiratory disease complex causes damage to lung tissue resulting in persistent lung lesions observable at slaughter. Severe lung lesions at harvest have been associated with decreased preharvest ADG and increased clinical BRDC in the feedlot. Our objective was to identify SNP that are associated with severe lung lesions observed at harvest in feedlot cattle. We conducted a genomewide association study (GWAS) using a case-control design for severe lung lesions in fed cattle at slaughter using the Illumina Bovine HD array (approximately 770,000 SNP) and sample pooling. Lung samples were collected from 11,520 young cattle, a portion of which had not been treated with antibiotics (participating in a "natural" marketing program), at a large, commercial beef processing plant in central Nebraska. Lung samples with lesions (cases) and healthy lungs (controls) were collected when both phenotypes were in close proximity on the viscera (offal) table. We constructed 60 case and 60 control pools with 96 animals per pool. Pools were constructed by sam-pling sequence to ensure that case and control pool pairs were matched by proximity on the processing line. The Bovine HD array (770,000 SNP) was run on all pools. Fourteen SNP on BTA 2, 3, 4, 9, 11, 14, 15, 22, 24, and 25 were significant at the genomewide experiment-wise error rate of 5% (P <= 1.49 x 10(-7)). Eighty-five SNP on 28 chromosomes achieved a false discovery rate of 5% (P <= 5.38 x 10(-6)). Significant SNP were near (+/- 100 kb) genes involved in tissue repair and regeneration, tumor suppression, cell proliferation, apoptosis, control of organ size, and immunity. Based on 85 significantly associated SNP in or near a collection of genes with diverse function on 28 chromosomes, we conclude that the genomic footprint of lung lesions is complex. A complex genomic footprint (genes and regulatory elements that affect the trait) is consistent with what is known about the cause of the disease: complex interactions among multiple viral and bacterial pathogens along with several environmental factors including dust, commingling, transportation, and stress. Characterization of sequence variation near significant SNP will enable accurate and cost effective genome-enhanced genetic evaluations for BRDC resistance in AI bulls and seed stock populations. C1 [Keele, J. W.; Kuehn, L. A.; McDaneld, T. G.; Tait, R. G., Jr.; Jones, S. A.; Smith, T. P. L.; Shackelford, S. D.; King, D. A.; Wheeler, T. L.; Lindholm-Perry, A. K.; McNeel, A. K.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Keele, JW (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM john.keele@ars.usda.gov NR 31 TC 3 Z9 3 U1 3 U2 11 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2015 VL 93 IS 3 BP 956 EP 964 DI 10.2527/jas.2014-8492 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6PK UT WOS:000357089100016 PM 26020874 ER PT J AU Psaros, KM McDaneld, TG Kuehn, LA Snelling, WM Keele, JW AF Psaros, K. M. McDaneld, T. G. Kuehn, L. A. Snelling, W. M. Keele, J. W. TI Evaluation of single nucleotide polymorphisms in chromosomal regions impacting pregnancy status in cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; deoxyribonucleic acid pooling; genotyping; reproduction ID LINEAR MIXED MODELS; REPRODUCTIVE EFFICIENCY; VARIANCE COMPONENT; FEMALE CATTLE; ASSOCIATION; TRAITS; GENOME; TESTS; EXPRESSION; GROWTH AB Reproductive success is an important component of commercial beef cattle production, and identification of DNA markers with predictive merit for reproductive success would facilitate accurate prediction of mean daughter pregnancy rate, enabling effective selection of bulls to improve female fertility. A previous study identified SNP associated with beef cattle reproductive efficiency based on a genome-wide association analysis approach using genotyping multiple-animal pools of DNA to increase the number of animals that could be genotyped with available resources. For the current study, we expand on this previous study by individually genotyping cattle from the pooling study for 89 SNP that were associated with female pregnancy rate. The aims of the study were to confirm the results of the pooling study and, more specifically, identify modes of gene action and DNA variations such as haplotypes that would not be possible with pooled genotyping. Eighty-nine SNP selected from the pooling study were evaluated using the Sequenom MassARRAY system to individually genotype animals from populations evaluated in the pooling study, including both Bos indicus and Bos taurus breeds. From this research, regions on chromosomes 5 (26.3-48.1 Mb; UMD3.1 assembly) and 9 (37,436,575 bp; UMD3.1 assembly), first identified in the previous pooling study, were shown through individual genotyping to harbor genetic variation (P < 0.05 genomewide significance) affecting reproductive efficiency in interspecific crosses (Bos indicus and Bos taurus) of cattle. Each of these markers exhibited additive (vs. dominant) gene action. Additionally, a haplotype block harboring an allele of Bos indicus origin with negative effects on reproduction was identified on chromosome 5 in interspecific composite breeds of Bos indicus x Bos taurus composites. C1 [Psaros, K. M.] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [McDaneld, T. G.; Kuehn, L. A.; Snelling, W. M.; Keele, J. W.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP McDaneld, TG (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM tara.mcdaneld@ars.usda.gov NR 32 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2015 VL 93 IS 3 BP 978 EP 987 DI 10.2527/jas.2014-8509 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6PK UT WOS:000357089100018 PM 26020876 ER PT J AU Diaz, JAC Vallet, JL Prince, TJ Phillips, CE DeDecker, AE Stalder, KJ AF Diaz, J. A. Calderon Vallet, J. L. Prince, T. J. Phillips, C. E. DeDecker, A. E. Stalder, K. J. TI Optimal dietary energy and amino acids for gilt development: Growth, body composition, feed intake, and carcass composition traits SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE body composition; carcass; feed intake; lysine; metabolizable energy; replacement gilts ID GROWING-PIGS; REPRODUCTIVE-PERFORMANCE; CULLING PATTERNS; FINISHING GILTS; LEAN GROWTH; LYSINE; PROTEIN; LEVEL; SWINE; KILOGRAMS AB The objective of this study was to determine if body composition of developing gilts could be altered at the onset of estrus by ad libitum feeding diets differing in standard ileal digestible (SID) lysine and ME using levels that are within those used in practice by pig producers in the United States. Crossbred Large White x Landrace gilts (n = 1,221), housed in groups, were randomly allotted to 6 corn-soybean diets in a 2 x 3 factorial arrangement formulated to provide 2 SID lysine and 3 ME levels. Gilts received grower diets formulated to provide 0.86 (low) or 1.02% (high) SID lysine and 2.94 (low), 3.25 (medium), or 3.57 (high) Mcal of ME/kg from 100 d of age until approximately 90 kg BW. Then, gilts were fed finisher diets containing 0.73 (low) or 0.85% (high) SID lysine and 2.94 (low), 3.26 (medium) or 3.59 (high) Mcal of ME/kg until 260 d of age. The medium SID lysine and medium-ME diets were based on an informal survey from the U.S. commercial swine industry to obtain average levels that are currently being formulated for developing gilts. Gilts were weighed and backfat thickness and loin area were recorded at the beginning of the trial and then every 28 d. Feed intake (FI) was recorded as feed disappearance within the pen at 2-wk intervals. Lysine (g) and ME (Mcal) consumed were calculated based on diet formulations. At approximately 260 d of age, gilts were slaughtered and warm carcass weight and fat thickness were recorded. There were no differences between lysine or ME levels for growth and body composition, except for backfat, which was slightly greater for gilts fed a high-ME diet. Gilts fed high-ME diets had a lower FI but a greater ME intake compared with gilts fed low ME (P < 0.05). Additionally, gilts fed the high-ME diet had lower FI and lysine intake per kilogram of BW gain when compared with gilts fed low-or medium-ME diets (P < 0.05). However, there was no difference in the megacalories consumed per kilogram of BW gain among treatments (P > 0.05). Carcasses from gilts fed the high-ME diet were 3.3 and 2.5 kg heavier than those from gilts fed the low- or medium-ME diets (P < 0.05). Despite significant differences in the lysine: ME ratio in the diets, no changes in growth or body composition occurred, likely due to compensatory changes in FI in response to dietary ME content. Caloric efficiency (Mcal to deposit 1 kg of BW) was similar among treatments. C1 [Diaz, J. A. Calderon; Stalder, K. J.] Iowa State Univ, Anim Sci, Ames, IA 50011 USA. [Vallet, J. L.] USDA ARS, US Meat Anim Res Ctr, Reprod Res Unit, Clay Ctr, NE 68933 USA. [Prince, T. J.] Prince Nutr Serv LLC, Auburn, AL 36830 USA. [Phillips, C. E.; DeDecker, A. E.] Murphy Brown LLC, Prod Res, Rose Hill, NC 28458 USA. RP Stalder, KJ (reprint author), Iowa State Univ, Anim Sci, 109 Kildee Hall, Ames, IA 50011 USA. EM stalder@iastate.edu OI Stalder, Kenneth/0000-0001-9540-681X FU National Pork Checkoff under the Sow Lifetime Productivity [12-209] FX This work was funded by the National Pork Checkoff under the Sow Lifetime Productivity Project number 12-209. The authors would like to thank Murphy Brown LLC for their collaboration during this project. NR 41 TC 2 Z9 2 U1 0 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2015 VL 93 IS 3 BP 1187 EP 1199 DI 10.2527/jas.2014-8460 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6PK UT WOS:000357089100038 ER PT J AU Peterson, CM Pilcher, CM Rothe, HM Marchant-Forde, JN Ritter, MJ Carr, SN Puls, CL Ellis, M AF Peterson, C. M. Pilcher, C. M. Rothe, H. M. Marchant-Forde, J. N. Ritter, M. J. Carr, S. N. Puls, C. L. Ellis, M. TI Effect of feeding ractopamine hydrochloride on growth performance and responses to handling and transport in heavy-weight pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE growth; handling and transport; pigs; ractopamine ID FINISHING PIGS; FLOOR SPACE; LOSSES; BEHAVIOR AB The impact of feeding ractopamine hydrochloride (RAC) on growth performance and responses to handling and transport in heavy BW pigs was evaluated in a study performed as a split-plot design with a 3 x 3 factorial arrangement of treatments: 1) RAC level (0 vs. 5 vs. 7.5 mg/kg of feed) and 2) handling intensity (HI; gentle vs. moderate vs. aggressive); RAC level was the main plot and HI was the subplot. A total of 288 pigs housed in groups of 8 were used to evaluate growth performance over a 28-d RAC feeding period (98.5 +/- 4.58 to 131.5 +/- 7.45 kg BW). On d 29 of the study, the HI treatment was applied to 216 pigs (6/pen; 2/pen on each HI). This was followed by transportation for 1 h on a livestock trailer at the end of which pigs were subjected to a final handling procedure. Blood samples (to measure acid-base, cortisol, and catecholamine levels) were collected and rectal temperature was measured 2 h before the HI treatment (baseline) and after the final handling procedure (final). Feeding RAC (5 and 7.5 mg/kg) improved (P < 0.01) ADG (9.9 and 9.0% for 5 and 7.5 mg/kg RAC, respectively) and G:F (8.8 and 11.8%, respectively) compared to controls, with no differences (P > 0.05) between the 2 RAC levels. Increasing the intensity of handling decreased (P < 0.001) final blood pH, bicarbonate, and base excess and increased (P < 0.001) final blood lactate and plasma cortisol and norepinephrine levels. Aggressive compared to gentle handling increased (P < 0.05) the incidence of pigs exhibiting open-mouth breathing and skin discoloration after the final handling procedure but had no effect (P > 0.05) on the incidence on nonambulatory, noninjured pigs. There was no effect (P > 0.05) of feeding RAC on final rectal temperature or blood acid-base measurements. Feeding 7.5, but not 5, compared to 0 mg/kg RAC increased (P < 0.05) final plasma epinephrine levels and the incidence of nonambulatory, noninjured pigs. This study confirms the improved growth performance of pigs fed RAC and the negative effects of aggressive handling on physical, metabolic, and physiological responses of pigs. It also suggests that pigs fed 5 compared to 0 mg/kg RAC showed similar responses to transport and handling. However, pigs fed 7.5 mg/kg of RAC had a greater incidence of nonambulatory, noninjured pigs when subjected to the handling/transport model and this warrants further investigation. C1 [Peterson, C. M.; Pilcher, C. M.; Rothe, H. M.; Puls, C. L.; Ellis, M.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Marchant-Forde, J. N.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Ritter, M. J.; Carr, S. N.] Elanco Anim Hlth, Greenfield, IN 46140 USA. RP Ellis, M (reprint author), Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. EM mellis7@illinois.edu OI Marchant-Forde, Jeremy/0000-0002-5287-2914 NR 18 TC 1 Z9 1 U1 1 U2 10 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2015 VL 93 IS 3 BP 1239 EP 1249 DI 10.2527/jas.2014-8303 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6PK UT WOS:000357089100042 PM 26020900 ER PT J AU Santurtun, E Moreau, V Marchant-Forde, JN Phillips, CJC AF Santurtun, E. Moreau, V. Marchant-Forde, J. N. Phillips, C. J. C. TI Physiological and behavioral responses of sheep to simulated sea transport motions SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE balance; behavior; heart rate variability; sea motions; sheep; transport ID HEART-RATE-VARIABILITY; VESTIBULAR NUCLEUS; VEHICULAR MOTION; ROLL OSCILLATION; SICKNESS; ANIMALS; STRESS; WELFARE; ROAD; EXPRESSION AB The motion of ships can cause discomfort and stress in humans, but little is known about the impact on sheep welfare, despite many sheep traveling long distances by ship during live export. We tested whether exposing sheep to roll (side to side movement), heave (up and down movement), and pitch (front to back movement) with similar amplitude and period conditions to a commercial livestock transport vessel would affect their behavior and physiology. Specifically, we tested the effects of these motions and a control treatment on behavior, heart rate variability, rumination, body posture, and balance of sheep. Four sheep (37 +/- 0.1 kg) were restrained in pairs in a crate, which was placed on a moveable and programmable platform that generated roll and pitch motions. An electric forklift was used to produce heave motion. The treatments were applied for 30 min each time in a changeover design with 1 repetition over 8 consecutive days. Sheep behavior was recorded continuously from video records, and heart rate monitors were attached to determine heart rate and its variability. Heave reduced the time that sheep spent ruminating, compared with the other 3 treatments (P < 0.001). The 2 sheep spent more time during heave with their heads 1 above the head of the other (P < 0.001) and looking toward their companion (P = 0.02), indicating greater affiliative behavior. Sheep spent more time during heave standing with their back supported on the crate (P = 0.006) and less time lying down (P = 0.01). Roll caused more stepping motions than pitch and control, indicating loss of balance (P < 0.001). Sheep experiencing heave and roll had increased heart rates and reduced interbeat intervals (IBI) compared to the control (P < 0.001). The IBI of sheep in the heave treatment had an increased ratio of low to high frequency duration (P = 0.01), indicating reduced parasympathetic control of stress responses. Therefore, there was both behavioral and physiological evidence that heave and roll caused stress, with sheep experiencing roll apparently coping better by regular posture changes and heave causing the sheep to seek the close presence of their companion. C1 [Santurtun, E.; Moreau, V.; Phillips, C. J. C.] Univ Queensland, Ctr Anim Welf & Ethics, Sch Vet Sci, Gatton, Qld 4343, Australia. [Moreau, V.] La Salle Beauvais Inst Polytech, F-60000 Beauvais, France. [Marchant-Forde, J. N.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Santurtun, E (reprint author), Univ Queensland, Ctr Anim Welf & Ethics, Sch Vet Sci, Gatton, Qld 4343, Australia. EM eduardo.santurtunoliveros@uqcon-nect.edu.au OI Marchant-Forde, Jeremy/0000-0002-5287-2914 FU Humane Slaughter Association; Humane Society International FX The authors acknowledge financial assistance from the Humane Slaughter Association and the Humane Society International, advice from Assistant Professor Thomas Banhazi of University of Southern Queensland, and assistance with data recording from Mrs. Agnes van der Schot. NR 56 TC 3 Z9 3 U1 2 U2 25 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2015 VL 93 IS 3 BP 1250 EP 1257 DI 10.2527/jas.2014-8037 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6PK UT WOS:000357089100043 PM 26020901 ER PT J AU Folta, SC Lichtenstein, AH Seguin, RA Goldberg, JP Corbin, MA Wiker, N Gauker, J Chui, K Nelson, ME AF Folta, Sara C. Lichtenstein, Alice H. Seguin, Rebecca A. Goldberg, Jeanne P. Corbin, Marilyn A. Wiker, Nancy Gauker, Jodi Chui, Kenneth Nelson, Miriam E. TI The StrongWomen-Healthy Hearts program in Pennsylvania: RE-AIM analysis SO TRANSLATIONAL BEHAVIORAL MEDICINE LA English DT Article DE Dissemination; RE-AIM; Cardiovascular disease; Women; Physical activity; Nutrition ID PHYSICAL-ACTIVITY QUESTIONNAIRE; LIFE-STYLE; INTERVENTION; FRAMEWORK; DISEASE; IMPACT; FRUIT; WOMEN; RISK AB Dissemination of evidence-based programs is needed to reduce CVD risk among midlife and older women. The aim of this study is to examine the public health impact of StrongWomen-Healthy Hearts in Pennsylvania using the RE-AIM framework. Reach, adoption, implementation, and maintenance were assessed using qualitative and quantitative measures; effectiveness was assessed using a pretest-posttest within-participants design. Reach into the target population was 5 in 100,000. Compared to the target population, a greater percentage of participants were white, married, middle-class, and had a graduate degree. Effectiveness was demonstrated (weight loss -2.0 kg, p<0.001). Adoption among trained leaders was high (83.3 %), as was fidelity in implementation (average score 9.3 of 10). No leaders maintained the program. To increase impact of the StrongWomen-Healthy Hearts Program, it will be important to lower the costs and modify the recruitment and training strategies to better reach low-income and minority women. Such strategies may also improve program maintenance. C1 [Folta, Sara C.; Goldberg, Jeanne P.; Nelson, Miriam E.] Tufts Univ, Friedman Sch Nutr Sci & Policy, John Hancock Res Ctr Phys Act Nutr & Obes Prevent, Medford, MA 02155 USA. [Lichtenstein, Alice H.] Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA USA. [Seguin, Rebecca A.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Corbin, Marilyn A.; Wiker, Nancy] Penn State Cooperat Extens, University Pk, PA USA. [Gauker, Jodi] Chester Cty Econ Dev Council, Exton, PA USA. [Chui, Kenneth] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Medford, MA 02155 USA. RP Folta, SC (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, John Hancock Res Ctr Phys Act Nutr & Obes Prevent, Medford, MA 02155 USA. EM sara.folta@tufts.edu FU Centers for Disease Control and Prevention, Atlanta, Georgia [1R18DP002144] FX This study was funded by grant 1R18DP002144 from the Centers for Disease Control and Prevention, Atlanta, Georgia. The funder played no role in the design, conduct, or analysis of the study, nor in the interpretation and reporting of the study findings. The researchers were independent from the funder. All authors had full access to study data and can take responsibility for the integrity of the data and accuracy of the data analysis. NR 27 TC 4 Z9 4 U1 2 U2 5 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 1869-6716 EI 1613-9860 J9 TRANSL BEHAV MED JI Transl. Behav. Med. PD MAR PY 2015 VL 5 IS 1 BP 94 EP 102 DI 10.1007/s13142-014-0286-8 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CL2QF UT WOS:000356788400011 PM 25729458 ER PT J AU Mohapatra, JK Pandey, LK Rai, DK Das, B Rodriguez, LL Rout, M Subramaniam, S Sanyal, A Rieder, E Pattnaik, B AF Mohapatra, Jajati K. Pandey, Laxmi K. Rai, Devendra K. Das, Biswajit Rodriguez, Luis L. Rout, Manoranjan Subramaniam, Saravanan Sanyal, Aniket Rieder, Elizabeth Pattnaik, Bramhadev TI Cell culture adaptation mutations in foot-and-mouth disease virus serotype A capsid proteins: implications for receptor interactions SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID SWISS-MODEL WORKSPACE; ANTIGENIC VARIATION; OLIGOSACCHARIDE RECEPTOR; INTEGRIN; SEQUENCE; SPECIFICITY; EVOLUTION; SELECTION; RESIDUES; COMPLEX AB In this study we describe the adaptive changes fixed on the capsid of several foot-and-mouth disease virus serotype A strains during propagation in cell monolayers. Viruses passaged extensively in three cell lines (BHK-21, LFBK and IB-RS-2) consistently gained positively charged amino acids in the putative heparin-sulfate-binding pocket (VP2 beta E-beta F loop, VP1 C-terminus and VP3 beta-B knob) surrounding the fivefold symmetry axis (VP1 beta-beta G loop) and at other discrete sites on the capsid (VP3 beta G-beta H loop, VP1 C-terminus, VP2 beta C strand and VP1 beta G-beta H loop). A lysine insertion in the VP1 beta F-beta G loop of two of the BHK-21-adapted viruses supports the biological advantage of positively charged residues acquired in cell culture. The charge transitions occurred irrespective of cell line, suggesting their possible role in ionic interaction with ubiquitous negatively charged cell-surface molecules such as glycosaminoglycans (GAG). This was supported by the ability of the cell-culture-adapted variants to replicate in the integrin-deficient, GAG-positive CHO-K1 cells and their superior fitness in competition assays compared with the lower passage viruses with WT genotypes. Substitutions fixed in the VP1 beta G-beta H loop (-3, 2 and +2 'RGD' positions) or in the structural element known to be juxtaposed against that loop (VP1 beta B-beta C loop) suggest their possible role in modulating the efficiency and specificity of interaction of the 'RGD' motif with alpha(v)-integrin receptors. The nature and location of the substitutions described in this study could be applied in the rapid cell culture adaptation of viral strains for vaccine production. C1 [Mohapatra, Jajati K.; Pandey, Laxmi K.; Das, Biswajit; Rout, Manoranjan; Subramaniam, Saravanan; Sanyal, Aniket; Pattnaik, Bramhadev] Project Directorate Foot & Mouth Dis, Mukteswar 263138, Uttarakhand, India. [Rai, Devendra K.; Rodriguez, Luis L.; Rieder, Elizabeth] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. RP Mohapatra, JK (reprint author), Project Directorate Foot & Mouth Dis, IVRI Campus, Mukteswar 263138, Uttarakhand, India. EM jajati1@gmail.com; pattnaikb@gmail.com FU Indian Council of Agricultural Research FX This work was supported by the Indian Council of Agricultural Research. NR 35 TC 1 Z9 4 U1 0 U2 1 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD MAR PY 2015 VL 96 BP 553 EP 564 DI 10.1099/vir.0.071597-0 PN 3 PG 12 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CJ8WT UT WOS:000355784900008 PM 25381054 ER PT J AU Smith, RJ Benavides, JC Jovan, S Amacher, M McCune, B AF Smith, Robert J. Benavides, Juan C. Jovan, Sarah Amacher, Michael McCune, Bruce TI A rapid method for landscape assessment of carbon storage and ecosystem function in moss and lichen ground layers SO BRYOLOGIST LA English DT Article DE Biomass; boreal forests; bryophyte and lichen ecology; carbon sequestration and cycling; climate change; ecosystem functions; Forest Inventory and Analysis program; land-use change; soil nutrient cycles ID BIOLOGICAL SOIL CRUSTS; BLACK SPRUCE FORESTS; ALASKA BOREAL FOREST; DOUGLAS-FIR FOREST; CLIMATE-CHANGE; PEATLAND HYDROLOGY; ARCTIC ECOSYSTEMS; PACIFIC-NORTHWEST; INTERIOR ALASKA; FEATHER MOSS AB Mat-forming "ground layers" of mosses and lichens often have functional impacts disproportionate to their biomass, and are responsible for sequestering one-third of the world's terrestrial carbon as they regulate water tables, cool soils and inhibit microbial decomposition. Without reliable assessment tools, the potential effects of climate and land use changes on these functions remain unclear; therefore, we implemented a novel "Ground Layer Indicator" method as part of the U.S.D.A. Forest Inventory and Analysis (FIA) program. Non-destructive depth and cover measurements were used to estimate biomass, carbon and nitrogen content for nine moss and lichen functional groups among eight contrasted habitat types in Pacific Northwest and subarctic U.S.A. (N = 81 sites). Ground layer cover, volume, standing biomass, carbon content and functional group richness were greater in boreal forest and tundra habitats of Alaska compared to Oregon forest and steppe. Biomass of up to 22769 +/- 2707 kg ha(-1) (mean 6 SE) in upland Picea mariana forests was nearly double other reports, likely because our method included viable, non-photosynthetic tissues. Functional group richness, which did not directly correspond with biomass, was greatest in lowland Picea mariana forests (7.1 +/- 0.4 functional groups per site). Bootstrap resampling revealed that thirty-two microplots per site were adequate for meeting data quality objectives. Here we present a non-destructive, repeatable and efficient method (sampling time: ca. 60 min per site) for gauging ground layer functions and evaluating responses to ecosystem changes. High biomass and functional distinctiveness in Alaskan ground layers highlight the need for increased attention to currently under-sampled boreal and arctic regions, which are projected to be among the most active responders to climate change. C1 [Smith, Robert J.; McCune, Bruce] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Benavides, Juan C.] Medellin Bot Garden, Medellin, Colombia. [Jovan, Sarah] US Forest Serv, USDA, Portland Forestry Sci Lab, Portland, OR 97205 USA. [Amacher, Michael] US Forest Serv, USDA, Logan Forestry Sci Lab, Logan, UT 84321 USA. RP Smith, RJ (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, 2082 Cordley Hall, Corvallis, OR 97331 USA. EM smithr2@onid.oregonstate.edu OI Smith, Robert/0000-0001-5085-634X FU USDA Forest Service [12-JVA-11261979]; Oregon State University [12-JVA-11261979] FX We are grateful to Trish Wurtz and Teresa Hollingsworth for field assistance and advising; Doug Daoust and Allison Nelson for project support; Bethany Schulz, Andrew Gray, and Robert Pattison for advising on field method, Kaleigh Spickerman for comments; Nick Lisuzzo and Shalane Frost for field assistance in Alaska; and Jamie Hollingsworth for facilitating surveys at Bonanza Creek Experimental Forest. Comments by the editor and two anonymous reviewers improved the quality of the manuscript. This research was supported by Joint Venture Agreement 12-JVA-11261979 between the USDA Forest Service and Oregon State University. NR 82 TC 1 Z9 1 U1 17 U2 69 PU AMER BRYOLOGICAL LICHENOLOGICAL SOC INC PI OMAHA PA C/O DR ROBERT S EGAN, SEC-TRES, ABLS, UNIV NEBRASKA OMAHA, DEPT BIOLOGY, OMAHA, NE 68182-0040 USA SN 0007-2745 EI 1938-4378 J9 BRYOLOGIST JI Bryologist PD SPR PY 2015 VL 118 IS 1 BP 32 EP 45 DI 10.1639/0007-2745-118.1.032 PG 14 WC Plant Sciences SC Plant Sciences GA CH7NV UT WOS:000354224700004 ER PT J AU Will-Wolf, S Jovan, S Neitlich, P Peck, JE Rosentreter, R AF Will-Wolf, Susan Jovan, Sarah Neitlich, Peter Peck, JeriLynn E. Rosentreter, Roger TI Lichen-based indices to quantify responses to climate and air pollution across northeastern USA SO BRYOLOGIST LA English DT Article DE Air pollution; air quality; climate; forest; indicator species; lichen; nitrogen; northeastern USA; sulfur ID EPIPHYTIC MACROLICHEN COMMUNITIES; WESTERN UNITED-STATES; LONG-TERM CHANGES; NITROGEN DEPOSITION; LANDSCAPE PATTERN; GRADIENTS; FORESTS; INDICATORS; CALIFORNIA; GROWTH AB Lichens are known to be indicators for air quality; they also respond to climate. We developed indices for lichen response to climate and air quality in forests across the northeastern United States of America (U.S.A.), using 218-250 plot surveys with 145-161 macrolichen taxa from the Forest Inventory and Analysis (FIA) Program of the U.S. Department of Agriculture, Forest Service. Lichen indicator species for response to climate and air quality were selected using Indicator Species Analysis, correlations with environmental variables, and published literature. Ordinations were used to evaluate the strength and relationships of the final indices. The Pollution Index was calculated for a plot from abundances of 12 tolerant and 45 sensitive indicator species standardized by abundance of all lichen species. The Index was correlated with modeled deposition of acidifying sulfur and oxidized nitrogen and with lichen community ordination pollution axes. Analyses suggested separate response of lichens to fertilizing N (weak statistical support). The Climate Index, from abundances of 19 warmer and 47 cooler climate indicator species, was correlated with modeled minimum January and annual maximum temperatures, and with ordination climate axes. The two indices are statistically independent. Repeat sample variability for each index was 714.5% (lower with higher quality data), supporting detection of consistent trends of 16-20% change over time or variation across space. Variability of the Climate Index was more affected by data quality than that of the Pollution Index. The continuous gradient of Pollution Index values suggests the cleanest areas may have air pollution above a critical load to fully protect lichen communities. These Indices can be applied to track lichen responses using other data from our study regions; suitability should be tested before use outside of the study area. C1 [Will-Wolf, Susan] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA. [Jovan, Sarah] US Forest Serv, USDA, Portland Forestry Sci Lab, Portland, OR 97205 USA. [Neitlich, Peter] Natl Pk Serv, Western Arctic Natl Parklands, Winthrop, WA 98862 USA. [Peck, JeriLynn E.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Rosentreter, Roger] Boise State Univ, Dept Biol, Boise, ID 83642 USA. RP Will-Wolf, S (reprint author), Univ Wisconsin, Dept Bot, 430 Lincoln Dr, Madison, WI 53706 USA. EM swwolf@wisc.edu FU USDA Forest Service Forest Health Monitoring Program (FHM) [23-94-29]; University of Wisconsin-Madison [23-94-29, SRS00-CA-11220146-082, SRS09-CA1130145101]; Oregon State University (original funds from USDA FS FHM) [2-5310-02]; USDA Forest Service Forest Indicator and Analysis Program (FIA) [SRS00-CA-11220146-082, SRS09-CA1130145101] FX This project was funded by cooperative agreement 23-94-29 between the USDA Forest Service Forest Health Monitoring Program (FHM) and University of Wisconsin-Madison, 1995 subcontract 2-5310-02 from Oregon State University to University of Wisconsin-Madison (original funds from USDA FS FHM), and cooperative agreements SRS00-CA-11220146-082 and SRS09-CA1130145101 between the USDA Forest Service Forest Indicator and Analysis Program (FIA) and University of Wisconsin-Madison, all for research by Susan Will-Wolf. FHM and FIA Northern Region field crews collected most of the plot and lichen data used for this project; their contributions were invaluable. We thank Bruce McCune for assistance describing the mathematical properties of our lichen abundance index, which he designed. We thank Linda Geiser and an anonymous reviewer for many comments that improved the paper. NR 90 TC 5 Z9 5 U1 5 U2 26 PU AMER BRYOLOGICAL LICHENOLOGICAL SOC INC PI OMAHA PA C/O DR ROBERT S EGAN, SEC-TRES, ABLS, UNIV NEBRASKA OMAHA, DEPT BIOLOGY, OMAHA, NE 68182-0040 USA SN 0007-2745 EI 1938-4378 J9 BRYOLOGIST JI Bryologist PD SPR PY 2015 VL 118 IS 1 BP 59 EP 82 DI 10.1639/0007-2745-118.1.059 PG 24 WC Plant Sciences SC Plant Sciences GA CH7NV UT WOS:000354224700007 ER PT J AU Morris, CF Casper, J Kiszonas, AM Fuerst, EP Murray, J Simeone, MC Lafiandra, D AF Morris, C. F. Casper, J. Kiszonas, A. M. Fuerst, E. P. Murray, J. Simeone, M. C. Lafiandra, D. TI Soft Kernel Durum Wheat-A New Bakery Ingredient? SO CEREAL FOODS WORLD LA English DT Article ID HEAT-STRESS; BREADMAKING QUALITY; PROTEIN-COMPOSITION; TRITICUM-AESTIVUM; GLUTEN STRENGTH; GRAIN-YIELD; BREAD; GENOTYPES; PUROINDOLINES; CULTIVARS C1 [Morris, C. F.; Kiszonas, A. M.] Washington State Univ, USDA ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA. [Casper, J.] Cargill Oils & Shortenings R&D, Plymouth, MA USA. [Fuerst, E. P.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Murray, J.] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Simeone, M. C.; Lafiandra, D.] Univ Tuscia, Dept Agr Forestry Nat & Energy, Viterbo, Italy. RP Morris, CF (reprint author), Washington State Univ, USDA ARS, Western Wheat Qual Lab, E-202 Food Qual Bldg, Pullman, WA 99164 USA. EM morrisc@wsu.edu FU NIFA AFRI [2013-67013-21226]; OECD Grant [JA00080722] FX We thank Leonard Joppa, Jodi A. Engleson, Kim Shantz, Dale Clark, Sam Huang, Karim Ammar, Elias Elias, Walter von Reding, Bill Atwell, Mehmet Tulbek, and Gabriel Burns; the staff of the WWQL, Doug Engle, Gail Jacobsen, Mary Baldridge, Wm. Kelley, Janet Luna, Mishelle Lenssen, Patricia Boyer, and Bozena Paszczynska (deceased); Joel Abecassis and Valerie Lullien-Pellerin (INRA, Montpellier, France); Claudio Pagliaccia (Fornovecchino); and Art and Doug McIntosh and Renae Schmidt (Harvest Ridge Organics). Stacey Sykes and Shawna Vogl assisted in the preparation of this manuscript. This research was supported in part by NIFA AFRI Grant No. 2013-67013-21226 and OECD Grant JA00080722. NR 28 TC 3 Z9 3 U1 2 U2 5 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0146-6283 J9 CEREAL FOOD WORLD JI Cereal Foods World PD MAR-APR PY 2015 VL 60 IS 2 BP 76 EP 83 DI 10.1094/CFW-60-2-0076 PG 8 WC Food Science & Technology SC Food Science & Technology GA CI4XL UT WOS:000354757100003 ER PT J AU Elmore, MH McGary, KL Wisecaver, JH Slot, JC Geiser, DM Sink, S O'Donnell, K Rokas, A AF Elmore, M. Holly McGary, Kriston L. Wisecaver, Jennifer H. Slot, Jason C. Geiser, David M. Sink, Stacy O'Donnell, Kerry Rokas, Antonis TI Clustering of Two Genes Putatively Involved in Cyanate Detoxification Evolved Recently and Independently in Multiple Fungal Lineages SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE Fusarium oxysporum; horizontal gene transfer; metabolic gene cluster; convergent evolution; gene duplication; fungicide resistance ID COMPARATIVE GENOMICS; SEQUENCE ALIGNMENT; DATABASE; CYANIDE; FUSARIUM; SEARCHES; BINDING AB Fungi that have the enzymes cyanase and carbonic anhydrase show a limited capacity to detoxify cyanate, a fungicide employed by both plants and humans. Here, we describe a novel two-gene cluster that comprises duplicated cyanase and carbonic anhydrase copies, which we name the CCA gene cluster, trace its evolution across Ascomycetes, and examine the evolutionary dynamics of its spread among lineages of the Fusarium oxysporum species complex (hereafter referred to as the FOSC), a cosmopolitan clade of purportedly clonalvascular wilt plant pathogens. Phylogenetic analysis of fungal cyanase and carbonic anhydrase genes reveals that the CCA gene cluster arose independently at least twice and is now present in three lineages, namely Cochliobolus lunatus, Oidiodendron maius, and the FOSC. Genome-wide surveys within the FOSC indicate that the CCA gene cluster varies in copy number across isolates, is always located on accessory chromosomes, and is absent in FOSC's closest relatives. Phylogenetic reconstruction of the CCA gene cluster in 163 FOSC strains from a wide variety of hosts suggests a recent history of rampant transfers between isolates. We hypothesize that the independent formation of the CCA gene cluster in different fungal lineages and its spread across FOSC strains may be associated with resistance to plant-produced cyanates or to use of cyanate fungicides in agriculture. C1 [Elmore, M. Holly; McGary, Kriston L.; Wisecaver, Jennifer H.; Slot, Jason C.; Rokas, Antonis] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Sink, Stacy; O'Donnell, Kerry] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Rokas, A (reprint author), Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. EM antonis.rokas@vanderbilt.edu RI Rokas, Antonis/A-9775-2008; Slot, Jason/E-4802-2011 OI Rokas, Antonis/0000-0002-7248-6551; FU Vanderbilt Undergraduate Summer Research Program Fellowship; National Science Foundation [2013170866, DEB-0844968, DEB-1442113] FX The authors thank Nathane Orwig for running some of the DNA sequences in the NCAUR DNA Core Facility. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. This work was conducted in part using the resources of the Advanced Computing Center for Research and Education at Vanderbilt University, Nashville, TN. This work was supported by funds provided by a Vanderbilt Undergraduate Summer Research Program Fellowship to M.H.E. and the National Science Foundation (2013170866 to M.H.E. and DEB-0844968 and DEB-1442113 to A.R.). NR 38 TC 5 Z9 5 U1 1 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD MAR PY 2015 VL 7 IS 3 BP 789 EP 800 DI 10.1093/gbe/evv025 PG 12 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA CI4UW UT WOS:000354750300014 PM 25663439 ER PT J AU Ma, C Meir, S Xiao, LT Tong, JH Liu, Q Reid, MS Jiang, CZ AF Ma, Chao Meir, Shimon Xiao, Langtao Tong, Jianhua Liu, Qing Reid, Michael S. Jiang, Cai-Zhong TI A KNOTTED1-LIKE HOMEOBOX Protein Regulates Abscission in Tomato by Modulating the Auxin Pathway SO PLANT PHYSIOLOGY LA English DT Article ID FLORAL ORGAN ABSCISSION; RECEPTOR-LIKE KINASE; FLOWER ABSCISSION; ARABIDOPSIS-THALIANA; INFLORESCENCE-DEFICIENT; SOLANUM-LYCOPERSICON; PLANT DEVELOPMENT; ZONE DEVELOPMENT; CELL-SEPARATION; KNOX GENES AB A gene encoding a KNOTTED1-LIKE HOMEOBOX PROTEIN1 (KD1) is highly expressed in both leaf and flower abscission zones. Reducing the abundance of transcripts of this gene in tomato (Solanum lycopersicum) by both virus-induced gene silencing and stable transformation with a silencing construct driven by an abscission-specific promoter resulted in a striking retardation of pedicel and petiole abscission. In contrast, Petroselinum, a semidominant KD1 mutant, showed accelerated pedicel and petiole abscission. Complementary DNA microarray and quantitative reverse transcription-polymerase chain reaction analysis indicated that regulation of abscission by KD1 was associated with changed abundance of genes related to auxin transporters and signaling components. Measurement of auxin content and activity of a DR5::beta-glucuronidase auxin reporter assay showed that changes in KD1 expression modulated the auxin concentration and response gradient in the abscission zone. C1 [Ma, Chao; Reid, Michael S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Meir, Shimon] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, IL-50250 Bet Dagan, Israel. [Xiao, Langtao; Tong, Jianhua; Liu, Qing] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China. [Jiang, Cai-Zhong] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. RP Reid, MS (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM msreid@ucdavis.edu; cjiang@ucdavis.edu RI Xiao, Langtao/K-7012-2016 OI Xiao, Langtao/0000-0003-1786-1950 FU U.S.-Israel Binational Agricultural Research and Development Fund [IS-4073-08C]; National Natural Science Foundation of China [91317312] FX This work was supported by the U.S.-Israel Binational Agricultural Research and Development Fund (grant no. IS-4073-08C) and the National Natural Science Foundation of China (grant no. 91317312). NR 46 TC 8 Z9 8 U1 4 U2 33 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD MAR PY 2015 VL 167 IS 3 BP 844 EP + DI 10.1104/pp.114.253815 PG 14 WC Plant Sciences SC Plant Sciences GA CI0GC UT WOS:000354413900020 PM 25560879 ER PT J AU Kondo, MC Low, SC Henning, J Branas, CC AF Kondo, Michelle C. Low, Sarah C. Henning, Jason Branas, Charles C. TI The Impact of Green Stormwater Infrastructure Installation on Surrounding Health and Safety SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID IN-DIFFERENCES ANALYSIS; INNER-CITY; DEFENSIBLE SPACE; DRUG-USE; CRIME; ENVIRONMENT; COCAINE; FEAR; ATTENTION; EXPOSURE AB Objectives. We investigated the health and safety effects of urban green stormwater infrastructure (GSI) installments. Methods. We conducted a difference-in-differences analysis of the effects of GSI installments on health (e.g., blood pressure, cholesterol and stress levels) and safety (e.g., felonies, nuisance and property crimes, narcotics crimes) outcomes from 2000 to 2012 in Philadelphia, Pennsylvania. We used mixed-effects regression models to compare differences in pre- and posttreatment measures of outcomes for treatment sites (n = 52) and randomly chosen, matched control sites (n = 186) within multiple geographic extents surrounding GSI sites. Results. Regression-adjusted models showed consistent and statistically significant reductions in narcotics possession (18%-27% less) within 16th mile, quarter-mile, half-mile (P < .001), and eighth-mile (P < .01) distances from treatment sites and at the census tract level (P < .01). Narcotics manufacture and burglaries were also significantly reduced at multiple scales. Nonsignificant reductions in homicides, assaults, thefts, public drunkenness, and narcotics sales were associated with GSI installation in at least 1 geographic extent. Conclusions. Health and safety considerations should be included in future assessments of GSI programs. Subsequent studies should assess mechanisms of this association. C1 [Kondo, Michelle C.; Low, Sarah C.; Henning, Jason] USDA, Forest Serv, No Res Stn, Philadelphia, PA 19103 USA. [Kondo, Michelle C.; Branas, Charles C.] Univ Penn, Ctr Clin Epidemiol & Biostat, Perelman Sch Med, Philadelphia, PA 19104 USA. [Henning, Jason] Davey Trees Inc, Philadelphia, PA USA. RP Kondo, MC (reprint author), USDA, Forest Serv, 100 North 20th St,Suite 205, Philadelphia, PA 19103 USA. EM michelleckondo@fs.fed.us OI Kondo, Michelle/0000-0001-9152-1589 FU US Department of Agriculture, Forest Service, Northern Research Station; National Institutes of Health [R01AA020331] FX This work was supported by the US Department of Agriculture, Forest Service, Northern Research Station, and the National Institutes of Health (grant R01AA020331 to C. C. B.). NR 46 TC 6 Z9 6 U1 4 U2 22 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD MAR PY 2015 VL 105 IS 3 BP E114 EP E121 DI 10.2105/AJPH.2014.302314 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CH5DP UT WOS:000354055100016 PM 25602887 ER PT J AU Ingham, RE Kroese, D Zasada, IA AF Ingham, Russell E. Kroese, Duncan Zasada, Inga A. TI Effect of Storage Environment on Hatching of the Cyst Nematode Globodera ellingtonae SO JOURNAL OF NEMATOLOGY LA English DT Article DE diapause; dormancy; hatching; potato root diffusate; quiescence; rearing; senescence; storage ID POTATO; ROSTOCHIENSIS; DIAPAUSE; MULTIPLICATION; TEMPERATURE; POPULATIONS; DAYLENGTH; OREGON AB Globodera spp. eggs go through a diapause, which remains dormant until favorable hatching conditions are reached. Because of the regulatory concerns with cyst nematodes, it is often only possible to rear eggs for research in the greenhouse. However, hatch is often lower for greenhouse-produced eggs than for eggs obtained from the field. The goal of this research was to determine storage conditions for Globodera ellingtonae eggs produced in the greenhouse that would increase percentage hatch. Over 3 yr, G. ellingtonae greenhouse-produced eggs were stored in different environments (-20 degrees C, 4 degrees C, room temperature, and the field) in either dry or moist soil. Percentage hatch after exposure to the different environments was determined in potato root diffusate. Across two experiments, field-produced eggs had higher hatch rates (65.2%) than greenhouse-produced eggs (10.4%). Temperature did not have an appreciable influence on hatch of eggs stored dry in two experiments (2.8% to 8.4% and 3.8% to 8.6%), but hatch of eggs stored in moist soil was significantly higher than in dry soil at all temperatures except -20 degrees C (26.8% and 28.7%). However, the ability of G. ellingtonae greenhouse-, microplot-, and field-produced eggs to reproduce on potato in field microplots was not different. Although it may not be possible to produce G. ellingtonae eggs in the greenhouse that have the magnitude of hatch as those produced in the field, hatching can be greatly increased by storing eggs in moist soil at either 4 degrees C or room temperature. C1 [Ingham, Russell E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Kroese, Duncan; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Ingham, RE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Cordley 2082, Corvallis, OR 97331 USA. EM inghamr@science.oregonstate.edu NR 22 TC 0 Z9 0 U1 6 U2 10 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2015 VL 47 IS 1 BP 45 EP 51 PG 7 WC Zoology SC Zoology GA CG9II UT WOS:000353628000004 PM 25861115 ER PT J AU Dinh, PTY Zhang, LH Mojtahedi, H Brown, CR Elling, AA AF Dinh, Phuong T. Y. Zhang, Linhai Mojtahedi, Hassan Brown, Charles R. Elling, Axel A. TI Broad Meloidogyne Resistance in Potato Based on RNA Interference of Effector Gene 16D10 SO JOURNAL OF NEMATOLOGY LA English DT Article DE Effector; host-parasitic relationship; Meloidogyne arenaria; M. chitwoodi; M. hapla; M. incognita; M. javanica; potato (Solanum tuberosum); resistance; RNA interference ID ROOT-KNOT NEMATODE; MC16D10L CONFERS RESISTANCE; DOUBLE-STRANDED-RNA; CULTIVATED POTATO; M-HAPLA; CHITWOODI; SOLANUM; COLUMBIA; PLANTS; ARABIDOPSIS AB Root-knot nematodes (Meloidogyne spp.) are a significant problem in potato (Solanum tuberosum) production. There is no potato cultivar with Meloidogyne resistance, even though resistance genes have been identified in wild potato species and were introgressed into breeding lines. The objectives of this study were to generate stable transgenic potato lines in a cv. Russet Burbank background that carry an RNA interference (RNAi) transgene capable of silencing the 16D10 Meloidogyne effector gene, and test for resistance against some of the most important root-knot nematode species affecting potato, i.e., M. arenaria, M. chitwoodi, M. hapla, M. incognita, and M. javanica. At 35 days after inoculation (DAI), the number of egg masses per plant was significantly reduced by 65% to 97% (P<0.05) in the RNAi line compared to wild type and empty vector controls. The largest reduction was observed in M. hapla, whereas the smallest reduction occurred in M. javanica. Likewise, the number of eggs per plant was significantly reduced by 66% to 87% in M. arenaria and M. hapla, respectively, compared to wild type and empty vector controls (P<0.05). Plant-mediated RNAi silencing of the 16D10 effector gene resulted in significant resistance against all of the root-knot nematode species tested, whereas RMc1(blb), the only known Meloidogyne resistance gene in potato, did not have a broad resistance effect. Silencing of 16D10 did not interfere with the attraction of M. incognita second-stage juveniles to roots, nor did it reduce root invasion. C1 [Dinh, Phuong T. Y.; Elling, Axel A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Zhang, Linhai; Brown, Charles R.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Mojtahedi, Hassan] Washington State Dept Agr, Prosser, WA 99350 USA. RP Dinh, PTY (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM elling@wsu.edu FU U.S. Department of Agriculture, Washington State Department of Agriculture; Northwest Potato Research Consortium [0672]; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Hatch Project [WNP00744] FX The authors thank Dr. Debra Inglis for critical reading of an earlier version of the manuscript. Funding for this project was provided by U.S. Department of Agriculture, Washington State Department of Agriculture, and Northwest Potato Research Consortium. PPNS No. 0672, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Hatch Project No. WNP00744, Washington State University, Pullman, WA 99164. NR 34 TC 1 Z9 1 U1 6 U2 17 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2015 VL 47 IS 1 BP 71 EP 78 PG 8 WC Zoology SC Zoology GA CG9II UT WOS:000353628000008 PM 25861119 ER PT J AU Bramwell, L AF Bramwell, Lincoln TI Firefighters in the Sky 75 Years and Still Smokejumping over Montana SO MONTANA-THE MAGAZINE OF WESTERN HISTORY LA English DT Article C1 US Forest Serv, Washington, DC 20250 USA. RP Bramwell, L (reprint author), US Forest Serv, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MONTANA HISTORICAL SOC PI HELENA PA 225 N ROBERTS ST, HELENA, MT 59601 USA SN 0026-9891 J9 MONTANA JI Mont.-Mag. West. Hist. PD SPR PY 2015 VL 65 IS 1 BP 60 EP 69 PG 10 WC History SC History GA CH3MW UT WOS:000353935800004 ER PT J AU Sui, R Baggard, J AF Sui, R. Baggard, J. TI WIRELESS SENSOR NETWORK FOR MONITORING SOIL MOISTURE AND WEATHER CONDITIONS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Irrigation; Soil moisture; Wireless sensor network; Weather station ID CAPACITANCE PROBES; WATER CONTENT; REAL-TIME; IRRIGATION; AGRICULTURE AB A wireless sensor network (WSN) was built and deployed in three fields to monitor soil moisture status and collect weather, data for irrigation scheduling. The WSN consists of soil moisture sensors, weather sensors, wireless data loggers, and a wireless modem. Soil moisture sensors were installed at three depths below the ground surface in various locations across the fields. Weather sensors were mounted on a 3-m instrument tower. An antenna mount was designed and fabricated for use in the WSN. When field equipment such as a fertilizer or chemical applicator impacted the mount, the mount was capable of protecting the antenna from damage by the equipment. In the WSN, received radio signal strength of Em50R data logger decreased as the distance from the data logger to the receiver increased. It also decreased as the distance between the top of the plant canopy and the logger's antenna above the plant canopy decreased. The antenna of the Em50R logger required replacement above the plant canopy for effective data communication. The Em50G data logger was capable of transferring data as its antenna was inside the plant canopy. Using the WSN system, soil moisture and weather conditions including precipitation, solar radiation, wind speed, and humidity were measured every minute and the hourly averages were reported and stored at 1-h interval. The soil moisture data and weather data were automatically and wirelessly transmitted to the internet making the data available online. Data collected by the WSN have been used in irrigation scheduling research in cotton, corn and soybean crops. C1 [Sui, R.; Baggard, J.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Baggard, J (reprint author), USDA ARS, Crop Prod Syst Res Unit, POB 350, Stoneville, MS 38776 USA. EM ruixiu.sui@ars.usda.gov FU Cotton Incorporated (Cary, N.C.) FX This project was partially supported by Cotton Incorporated (Cary, N.C.). NR 18 TC 0 Z9 1 U1 1 U2 22 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2015 VL 31 IS 2 BP 193 EP 201 PG 9 WC Agricultural Engineering SC Agriculture GA CG9WR UT WOS:000353671300003 ER PT J AU Derksen, RC Canas, LA Ranger, CM Reding, ME AF Derksen, R. C. Canas, L. A. Ranger, C. M. Reding, M. E. TI THE EFFECT OF APPLICATION METHOD ON THE TEMPORAL AND SPATIAL DISTRIBUTION OF NEONICOTINOID INSECTICIDES IN GREENHOUSE ZINNIA AND IMPACT ON APHID POPULATIONS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Drench; Green peach aphid; Imidacloprid; Neonicotinoid; Sprays; Systemic insecticide; Thiamethoxam; Zinnia ID SPRAY APPLICATION TECHNIQUE; IVY POT PLANTS; DEPOSITION; POINSETTIA; HANDGUN; ANGLE; BOOM AB Greenhouse trials were designed to evaluate the effect the application technique would have on temporal and spatial movement of neonicotinoid insecticides imidacloprid and thiamethoxam through plant tissue. Mature zinnia elegans plants were treated by either a soil drench of neonicotinoid insecticide or foliar application on a single leaf at approximately the midpoint of the plant height. Caged adult aphids were placed on the underside of leaves at five plant heights. Leaves were collected at various post-treatment times along the primary stem of the zinnia to determine levels of insecticide using commercial ELISA kits. Aphid populations were assessed at the time leaves were sampled. Insecticide was detected in all sampled leaves in the drench treatments. Drench treatments reduced aphid populations at all foliar sample locations. On single leaf treated plants, aphid populations were only reduced at the treated leaf and very little insecticide movement was detected. Results demonstrate that the method of delivery of neonicotinoids can have a significant impact on the biological effectiveness. The lack of significant movement of neonicotinoids following foliar application demonstrates the need to provide good spray distribution across the target plant. Drench treatments may provide more effective application options than foliar treatments for treating difficult to reach target areas. C1 [Derksen, R. C.; Ranger, C. M.; Reding, M. E.] USDA, Wooster, OH USA. [Canas, L. A.] Ohio State Univ, Dept Entomol, Wooster, OH USA. RP Derksen, RC (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, 1680 Madison Ave, Wooster, OH 44691 USA. EM rich.derksen@ars.usda.gov NR 21 TC 0 Z9 0 U1 2 U2 10 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2015 VL 31 IS 2 BP 211 EP 216 PG 6 WC Agricultural Engineering SC Agriculture GA CG9WR UT WOS:000353671300005 ER PT J AU Baker, KD Hughs, E Foulk, J AF Baker, K. D. Hughs, E. Foulk, J. TI SPINDLE SPEED OPTIMIZATION FOR COTTON PICKERS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Fiber quality; Harvesting ID HARVEST SYSTEMS; YARN QUALITY AB There is concern that changes to the operating speed and size of spindles on cotton picker harvesters over the years have resulted in a general decrease in cotton fiber quality, especially spindle twists, preparation, and neps. Previous research showed that spindle speeds of 3000 and 4000 rpm had more detrimental effects on picker stalk loss, trash levels in seed cotton, and cotton fiber quality than a spindle speed of 2000 rpm. This study was conducted to check fiber quality effects between 2000 and 2800 rpm in order to further define the optimum spindle speed range. Field tests were conducted for the 2008 and 2009 crop years by the USDA, Agricultural Research Service, Southwestern Cotton Ginning Research Laboratory in Mesilla Park, New Mexico. Three cotton varieties were grown under furrow-irrigated conditions in southern New Mexico and harvested with a modified one-row cotton picker each year using a ground speed of 0.85 m s(-1) (1.9 miles h(-1)) and spindle speeds of 2000, 2200, 2500, and 2800 rpm. The tests were replicated four times. No significant differences in stalk losses in the field were found. Significantly more trash was present in seed cotton picked with spindle speeds of 2200, 2500, and 2800 rpm than in seed cotton picked with a spindle speed of 2000 rpm. Differences in trash content were eliminated by seed cotton cleaning (same for all treatments). HVI classing data showed no significant dfferences among spindle speed treatments. Differences due to spindle speed for AFIS nep count, short fiber count, trash count, dust count, and seed coat nep count were non-significant at each of the four locations tested in the yarn preparation process. Results from open-end spinning tests, including opening and cleaning waste, total card waste, ends down, yarn strength, yarn elongation, neps, thick places, and thin places showed no significant differences due to spindle speed. Based on these results, the 2000 rpm spindle speed is optimal because trash in the seed cotton is reduced which reduces ginning costs; however; only minimal differences are present at spindle speeds up to 2800 rpm. C1 [Baker, K. D.; Hughs, E.] ARS, USDA, Southwestern Cotton Ginning Res Lab, Mesilla Pk, NM USA. [Foulk, J.] FX Fibers LLC, Clemson, SC USA. RP Baker, KD (reprint author), 300 E Coll Dr,POB 576, Mesilla Pk, NM 88047 USA. EM kevibake@nmsu.edu FU Cotton, Incorporated, Cary, North Carolina FX The financial support of Cotton, Incorporated, Cary, North Carolina, for this project and the collaboration of the USDA, Agricultural Research Service Cotton Quality Research Unit, Clemson, South Carolina, for cotton fiber quality and spinability is greatly appreciated as well as was the assistance of Southwestern Cotton Ginning Research Laboratory staff - Fermin Alvarado, Paul Delgado, Arnold Gomez, Juan Gomez, Ernest Herrera, Tye Lightfoot, Mark Trujillo, and Kirk Zivkovich. NR 11 TC 2 Z9 2 U1 4 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2015 VL 31 IS 2 BP 217 EP 225 PG 9 WC Agricultural Engineering SC Agriculture GA CG9WR UT WOS:000353671300006 ER PT J AU Jabro, JD Iversen, WM Stevens, WB Evans, RG AF Jabro, J. D. Iversen, W. M. Stevens, W. B. Evans, R. G. TI SPATIAL AND TEMPORAL VARIABILITY OF SOIL PENETRATION RESISTANCE TRANSECTING SUGARBEET ROWS AND INTER-ROWS IN TILLAGE SYSTEMS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Compaction; Sugarbeet; Tillage; Variability ID NORFOLK LOAMY SAND; PHYSICAL-PROPERTIES; STRIP-TILLAGE; WATER; COMPACTION; FIELD; CORN AB Soil compaction has detrimental consequences on soil quality and plant root growth. Soil compaction is a variable property due to tillage in both space and time. A field study was conducted near Sidney, Montana in 2007 to evaluate spatial and temporal variations of soil penetration resistance (PR) on a 61-cm transect across the rows and inter-rows of sugarbeet on a clay loam in both conventional (CT) and strip tillage (ST) systems. A cone penetrometer was used to measure PR on a grid sampling scheme (5 cm horizontal x 2.5 cm vertical). The penetrometer was pressed into the soil every 5 cm along a 61-cm transect using a steel template bisecting the rows and inter-rows of sugarbeet. At each transect point, measurements were recorded at 2.5-cm depth increment to a depth of 30 cm. Soil PR measurements were recorded prior to planting (26 April), after the first cultivation (13 June), before harvest (3 October), and after harvest (4 October). Soil water contents were gravimetrically determined at the time of PR measurements. Soil PR was significantly greater in CT than in ST across the rows and inter-rows at the 0 to 30 cm depth for all sampling dates. Generally, soil PR increased with depth at every position on transect for all sampling dates under both tillage systems. Significant temporal variations in soil PR within each tillage system were observed throughout the growing season. Spatial variation data showed that less compaction was observed in crop rows compared to inter-rows under both tillage systems. Soil PR values increased as the growing season progressed and were highest prior to crop harvest, approaching values greater than 3.5 MPa below the soil surface. C1 [Jabro, J. D.; Iversen, W. M.; Stevens, W. B.; Evans, R. G.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Jabro, JD (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM jay.jabro@ars.usda.gov NR 36 TC 0 Z9 0 U1 1 U2 9 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAR PY 2015 VL 31 IS 2 BP 237 EP 246 PG 10 WC Agricultural Engineering SC Agriculture GA CG9WR UT WOS:000353671300008 ER PT J AU Gilbert, MJ Kik, M Miller, WG Duim, B Wagenaar, JA AF Gilbert, Maarten J. Kik, Marja Miller, William G. Duim, Birgitta Wagenaar, Jaap A. TI Campylobacter iguaniorum sp nova, isolated from reptiles SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID SPECIES DEFINITION; THERMOPHILIC CAMPYLOBACTER; FETUS; IDENTIFICATION; HUMANS; TESTS; PROKARYOTES; MATRIX AB During sampling of reptiles for members of the class Epsilonproteobacteria, strains representing a member of the genus Campylobacter not belonging to any of the established taxa were isolated from lizards and chelonians. Initial amplified fragment length polymorphism, PCR and 16S rRNA sequence analysis showed that these strains were most closely related to Campylobacter fetus and Campylobacter hyointestinalis. A polyphasic study was undertaken to determine the taxonomic position of five strains. The strains were characterized by 16S rRNA and atpA sequence analysis, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and conventional phenotypic testing. Whole-genome sequences were determined for strains 1485E(T). and 2463D, and the average nucleotide and amino acid identities were determined for these strains. The strains formed a robust phylogenetic clade, divergent from all other species of the genus Campylobacter. In contrast to most currently known members of the genus Campylobacter, the strains showed growth at ambient temperatures, which might be an adaptation to their reptilian hosts. The results of this study clearly show that these strains isolated from reptiles represent a novel species within the genus Campylobacter, for which the name Campylobacter iguaniorum sp. nov. is proposed. The type strain is 1485E(T) (=LMG 28143(T)=CCUG 66346(T)). C1 [Gilbert, Maarten J.; Duim, Birgitta; Wagenaar, Jaap A.] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, NL-3508 TD Utrecht, Netherlands. [Kik, Marja] Univ Utrecht, Fac Vet Med, Dept Pathobiol, NL-3508 TD Utrecht, Netherlands. [Miller, William G.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. [Duim, Birgitta; Wagenaar, Jaap A.] WHO Collaborating Ctr Campylobacter, OIE Reference Lab Campylobacteriosis, Utrecht, Netherlands. [Wagenaar, Jaap A.] Cent Vet Inst Wageningen UR, Lelystad, Netherlands. RP Gilbert, MJ (reprint author), Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Yalelaan 1, NL-3508 TD Utrecht, Netherlands. EM m.j.gilbert@uu.nl NR 32 TC 3 Z9 3 U1 3 U2 8 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD MAR PY 2015 VL 65 BP 975 EP 982 DI 10.1099/ijs.0.000048 PN 3 PG 8 WC Microbiology SC Microbiology GA CG9WF UT WOS:000353670100036 PM 25574036 ER PT J AU Zhao, Y Davis, RE Wei, W Lee, IM AF Zhao, Yan Davis, Robert E. Wei, Wei Lee, Ing-Ming TI Should 'Candidatus Phytoplasma' be retained within the order Acholeplasmatales? SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID SEQUENCE-VARIABLE MOSAICS; COMPLETE GENOME SEQUENCE; NAD(P) BIOSYNTHESIS; ORDINAL RANK; FAM-NOV; CLASSIFICATION; AUSTRALIENSE; PROKARYOTES; MOLLICUTES; LAIDLAWII AB Phytoplasmas are a diverse but phylogenetically coherent group of cell-wall-less bacteria affiliated with the class Mollicutes. Due to difficulties in establishing axenic culture, phytoplasmas were assigned to a provisional genus, 'Candidatus Phytoplasma', and the genus was embraced within the order Acholeplasmatales. However, phytoplasmas differ significantly from species of the genus Acholeplasma in their habitat specificities, modes of life, metabolic capabilities, genomic architectures, and phylogenetic positions. This communication describes the unique ecological, nutritional, biochemical, genomic and phylogenetic properties that distinguish phytoplasmas from species of the genus Acholeplasma and all other taxa in the class Mollicutes. Since such distinguishing properties of the phytoplasmas are not referable to the descriptions of the order Acholeplasmatales and of all other existing orders, namely Mycoplasmatales, Entomoplasmatales and Anaeroplasmatales, this communication raises the question of whether 'Candidatus Phytoplasma' should be retained in the order Acholeplasmatales or whether a novel provisional order and family should be created to accommodate the genus 'Ca. Phytoplasma'. C1 [Zhao, Yan; Davis, Robert E.; Wei, Wei; Lee, Ing-Ming] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. RP Zhao, Y (reprint author), ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. EM yan.zhao@ars.usda.gov NR 48 TC 9 Z9 9 U1 1 U2 13 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD MAR PY 2015 VL 65 BP 1075 EP 1082 DI 10.1099/ijs.0.000050 PN 3 PG 8 WC Microbiology SC Microbiology GA CG9WF UT WOS:000353670100048 PM 25574038 ER PT J AU Barros-Parada, W Knight, AL Fuentes-Contreras, E AF Barros-Parada, Wilson Knight, Alan L. Fuentes-Contreras, Eduardo TI Modeling codling moth (Lepidoptera: Tortricidae) phenology and predicting egg hatch in apple orchards of the Maule Region, Chile SO Chilean Journal of Agricultural Research LA English DT Article DE Biofix; Cydia pomonella; degree days; codlemone; Malus domestica; pear ester ID CYDIA-POMONELLA LEPIDOPTERA; CODLEMONE-BAITED TRAPS; WASHINGTON-STATE; SEX-PHEROMONE; INSECTICIDE RESISTANCE; DECISION-SUPPORT; PEST-MANAGEMENT; AZINPHOS-METHYL; KAIROMONE; POPULATIONS AB Studies were conducted in the Maule Region to characterize the phenology of the codling moth, Cydia pomonella (L.) The Predictive Extension Timing Estimator (PETE) and a logistic phenological model were validated with eight data sets of cumulative moth catches in sex pheromone (PH) and kairomone-baited traps and the cumulative occurrence of fruit injuries from apple (Malus domestica Borkh.) orchards during the 2009-2011 seasons. Second, the start of egg hatch was predicted from the first sustained male and female moth catches (biofix) in traps baited with pear ester (PE), PE+acetic acid (AA), PE+PH, and PH alone. Both phenological models fit data well except that the logistic provided a better fit than the PETE model of the phenology of egg hatch of the codling moth in the first generation, with a difference of 11 d between models in the prediction of 50% egg hatch. No significant difference was found between biofix dates established for males using either PH or PE+PH lures or for the biofix date based on female catches with PE+AA or PH+PE. The biofix established with the sustained female catch occurred nearly 11 d later than the male-based biofix. The use of a female biofix provided on average a 4-d improvement in the prediction of first egg hatch compared with the traditional use of a male biofix, but this difference was not significant. The use of PE+AA lures increased the proportion of cases when a female-based biofix could be established compared with the use of the PH+PE lure. C1 [Barros-Parada, Wilson; Fuentes-Contreras, Eduardo] Univ Talca, Fac Ciencias Agr, Talca, Chile. [Knight, Alan L.] USDA ARS, Wapato, WA 98951 USA. RP Fuentes-Contreras, E (reprint author), Univ Talca, Fac Ciencias Agr, Casilla 747, Talca, Chile. EM efuentes@utalca.cl RI Fuentes-Contreras, Eduardo/A-7595-2008; Barros, Wilson/I-5535-2013 OI Fuentes-Contreras, Eduardo/0000-0003-3567-3830; Barros, Wilson/0000-0003-3015-4927 FU FONDECYT [1071029]; Millenium Nucleus (Molecular Ecology and Evolutionary Applications in the Agroecosystem) [NC 120027] FX This work was funded by FONDECYT grant 1071029 to EFC, and Millenium Nucleus (Molecular Ecology and Evolutionary Applications in the Agroecosystem) grant NC 120027 NR 38 TC 2 Z9 2 U1 2 U2 13 PU INST INVESTIGACIONES AGROPECUARIAS PI CHILLAN PA CENTRO REGIONAL DE INVESTIGACION QUILAMAPU, CASILLA 426, CHILLAN, 00000, CHILE SN 0718-5839 J9 CHIL J AGR RES JI Chil. J. Agric. Res. PD MAR PY 2015 VL 75 IS 1 BP 57 EP 62 DI 10.4067/S0718-58392015000100008 PG 6 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA CG5ON UT WOS:000353341900008 ER PT J AU Hall, MB AF Hall, Mary Beth TI Determination of Dietary Starch in Animal Feeds and Pet Food by an Enzymatic-Colorimetric Method: Collaborative Study SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID FIBER; CARBOHYDRATE AB Starch, glycogen, maltooligosaccharides, and other alpha-1,4- and alpha-1,6-linked glucose carbohydrates, exclusive of resistant starch, are collectively termed "dietary starch". This nutritionally important fraction is increasingly measured for use in diet formulation for animals as it can have positive or negative effects on animal performance and health by affecting energy supply, glycemic index, and formation of fermentation products by gut microbes. AOAC Method 920.40 that was used for measuring dietary starch in animal feeds was invalidated due to discontinued production of a required enzyme. As a replacement, an enzymatic-colorimetric starch assay developed in 1997 that had advantages in ease of sample handling and accuracy compared to other methods was considered. The assay was further modified to improve utilization of laboratory resources and reduce time required for the assay. The assay is quasi-empirical: glucose is the analyte detected, but its release is determined by run conditions and specification of enzymes. The modified assay was tested in an AOAC collaborative study to evaluate its accuracy and reliability for determination of dietary starch in animal feedstuffs and pet foods. In the assay, samples are incubated in screw cap tubes with thermostable a-amylase in pH 5.0 sodium acetate buffer for 1 h at 100 degrees C with periodic mixing to gelatinize and partially hydrolyze alpha-glucan. Amyloglucosidase is added, and the reaction mixture is incubated at 50 degrees C for 2 h and mixed once. After subsequent addition of water, mixing, clarification, and dilution as needed, free + enzymatically released glucose are measured. Values from a separate determination of free glucose are subtracted to give values for enzymatically released glucose. Dietary starch equals enzymatically released glucose multiplied by 162/180 (or 0.9) divided by the weight of the as received sample. Fifteen laboratories that represented feed company, regulatory, research, and commercial feed testing laboratories analyzed 10 homogenous test materials representing animal feedstuffs and pet foods in duplicate using the dietary starch assay. The test samples ranged from 1 to 70% in dietary starch content and included moist canned dog food, alfalfa pellets, distillers grains, ground corn grain, poultry feed, low starch horse feed, dry dog kibbles, complete dairy cattle feed, soybean meal, and corn silage. The average within-laboratory repeatability SD (s(r)) for percentage dietary starch in the test samples was 0.49 with a range of 0.03 to 1.56, and among-laboratory repeatability SDs (s(R)) averaged 0.96 with a range of 0.09 to 2.69. The HorRat averaged 2.0 for all test samples and 1.9 for test samples containing greater than 2% dietary starch. The HorRat results are comparable to those found for AOAC Method 996.11, which measures starch in cereal products. It is recommended that the dietary starch method be accepted for Official First Action status. C1 USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Hall, MB (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM marybeth.hall@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service FX I thank Jan Pitas (U.S. Dairy Forage Research Center) for assistance in developing the dietary starch method and assistance with preparing and distributing materials for the study. I thank the Laboratory Methods & Services Committee of the Association of American Feed Control Officials for their orchestration of the effort for defining dietary starch and their support and input in this project. I thank Nancy Thiex, Larry Novotny, and the staff of the Olsen Biochemistry Laboratory at South Dakota State University for assistance in preparing the test samples. Special thanks go to Nancy Thiex for her invaluable guidance and assistance throughout the study. The U.S. Department of Agriculture, Agricultural Research Service provided funding for the materials used in the study. I also thank the following collaborators for their participation in this study:; Robin Johnson, Montana Department of Agriculture and Analytical Laboratory, Bozeman, MT NR 23 TC 5 Z9 5 U1 4 U2 17 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD MAR-APR PY 2015 VL 98 IS 2 BP 397 EP 409 DI 10.5740/jaoacint.15-012 PG 13 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA CG5EE UT WOS:000353313100023 PM 25905746 ER PT J AU Kuenen, LPS Siegel, JP AF Kuenen, L. P. S. Siegel, Joel P. TI Measure Your Septa Release Ratios: Pheromone Release Ratio Variability Affected by Rubber Septa and Solvent SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Volatile traps; Pheromone collection; Volatile ratios; Methylene chloride; Hexane ID ORIENTAL FRUIT MOTH; INSECT SEX-PHEROMONES; GRAPHOLITHA-MOLESTA; EVAPORATION RATES; LEPIDOPTERA; COLLECTION; TORTRICIDAE; FORMULATIONS; IDENTIFICATION; ADSORPTION AB The type of solvent and the volume used to load pheromone components onto rubber septa had significant effects on pheromone release ratios, the variability of those release ratios, and the recoverability of the volatile components during subsequent extraction with hexane. Volatile release ratios of synthetic Oriental fruit moth (OFM) pheromone and additional volatile compounds were determined using a gas chromatograph column as a volatile trap for rapid (a parts per thousand currency sign1 hr) analysis from individual rubber septa. Volatile compound solutions were prepared in hexane, pentane, CH2Cl2, and methyl tert-butyl ether, and a 10, 33, or 100 mu l aliquot of each solution was applied to rubber septa. Septa loaded with 100 mu l of CH2Cl2 emitted significantly (P < 0.05) higher alcohol: acetate (OH:Ac) ratios than septa loaded with the other solvents, which were all similar. Release ratios of the alcohol and acetate components of the OFM pheromone components were assessed over a 3 week period using septa loaded with each solvent. Regardless of loading solvent, the OFM OH:Ac ratios declined logarithmically over 3 weeks; however, the decay slope from septa loaded with CH2Cl2 solutions was different from those of the other three solvents, which were nearly all the same. A high variability in OH:Ac release ratios was measured overall, regardless of the solvent used or the volume it was applied in. Four compounds of near-equal mass: 1-dodecanol, 1-dodecanal, methyl decanoate, and tridecane emitted different release ratios dependent on the solvent, hexane or CH2Cl2, with which a septum was loaded. The more polar and the greater the mass of the test compound, the slower it was emitted from a septum regardless of solvent. These combined results plus comparisons to earlier reports, suggest that researchers should empirically assess the release ratios from septa to be used in bioassays rather than just reporting the type of septum, ratios of compounds applied and solvent used to prepare them. C1 [Kuenen, L. P. S.; Siegel, Joel P.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Kuenen, LPS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM bas.kuenen@ars.usda.gov FU California Pistachio Research Board FX We are indebted to Mathew Hicks for conducting the volatile collections and GC analyses. This work was funded in part by the California Pistachio Research Board. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 33 TC 1 Z9 1 U1 3 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD MAR PY 2015 VL 41 IS 3 BP 303 EP 310 DI 10.1007/s10886-015-0557-y PG 8 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CG6GW UT WOS:000353397400009 PM 25801328 ER PT J AU Wang, YL Li, F Zhuang, H Li, LH Chen, X Zhang, JH AF Wang, Yongli Li, Feng Zhuang, Hong Li, Lianghao Chen, Xiao Zhang, Jianhao TI Effects of Plant Polyphenols and alpha-Tocopherol on Lipid Oxidation, Microbiological Characteristics, and Biogenic Amines Formation in Dry-Cured Bacons SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antimicrobial activity; biogenic amines; dry-cured bacon; polyphenols; alpha-tocopherol ID GRAPE SEED EXTRACT; SHELF-LIFE; GREEN TEA; ANTIOXIDANT ACTIVITY; FERMENTED SAUSAGES; SODIUM-CHLORIDE; PORK SAUSAGES; MEAT-PRODUCTS; ACID; HISTAMINE AB Effects of plant polyphenols (tea polyphenol [TP], grape seed extract [GSE], and gingerol) and a-tocopherol on physicochemical parameters, microbiological counts, and biogenic amines were determined in dry-cured bacons at the end of ripening. Results showed that plant polyphenols and alpha-tocopherol significantly decreased pH, thiobarbituric acid reactive substances content, and total volatile basic nitrogen (TVBN) compared with the control (P < 0.05). Microbial counts and biogenic amine contents in dry-cured bacons were affected by plant polyphenols or alpha-tocopherol, with TP being the most effective (P < 0.05) in reducing aerobic plate counts, Enterobacteriaceae, Micrococcaceae, yeast, and molds, as well as in inhibiting formation of putrescine, cadaverine, tyramine, and spermine. Principal component analysis indicated that the first 2 principal components (PC) explained about 85.5% of the total variation. PC1 was related with physicochemical factors, parts of biogenic amines, and spoilage microorganisms, whereas PC2 grouped the TVBN, tyramine, 2-phenylethylamine, yeast, and molds. These findings suggest that plant polyphenols, especially TP, could be used to process dry-cured bacons to improve the quality and safety of finished products. C1 [Wang, Yongli; Chen, Xiao; Zhang, Jianhao] Natl Engineer Res Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Yongli; Chen, Xiao; Zhang, Jianhao] Minist Agr, Key Lab Meat Proc & Qual Control, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Yongli; Chen, Xiao; Zhang, Jianhao] Nanjing Agr Univ, Synerget Innovat Ctr Food Safety & Nutr, Nanjing 210095, Jiangsu, Peoples R China. Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China. [Zhang, Jianhao] ARS, Qual & Safety Assessment Res Unit, USDA, Athens, GA 30605 USA. RP Zhang, JH (reprint author), Natl Engineer Res Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. EM jh_zhangnjau@163.com FU Natl. Key Technology R&D Program in the 12th Five-Year Plan of China [2012BAD28B01]; Foundation of Natl. Natural Science Foundation of China [31201417]; China Postdoctoral Science Foundation [2013M540561] FX This study was supported by the Natl. Key Technology R&D Program in the 12th Five-Year Plan of China (Grant No. 2012BAD28B01), the Foundation of Natl. Natural Science Foundation of China (Grant No. 31201417), and China Postdoctoral Science Foundation (Grant No. 2013M540561). NR 46 TC 4 Z9 4 U1 6 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2015 VL 80 IS 3 BP C547 EP C555 DI 10.1111/1750-3841.12796 PG 9 WC Food Science & Technology SC Food Science & Technology GA CG1VW UT WOS:000353064400009 PM 25676047 ER PT J AU Stommel, JR Dumm, JM AF Stommel, John R. Dumm, Judith M. TI Coordinated Regulation of Biosynthetic and Regulatory Genes Coincides with Anthocyanin Accumulation in Developing Eggplant Fruit SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE delphinidin; gene regulation; Myb; Myc; Solanum melongena ID HELIX TRANSCRIPTION FACTORS; SOLANUM-MELONGENA L.; CAPSICUM-ANNUUM; EVOLUTION; COLOR; EXPRESSION; PETUNIA; MYB; LC; PATHWAY AB Violet to black pigmentation of eggplant (Solanum melongena L.) fruit is caused by anthocyanin accumulation. Model systems demonstrate the role of regulatory genes in the control of anthocyanin biosynthesis. Anthocyanin structural gene transcription requires the expression of at least one member of each of three transcription factor families: MYB, MYC, and WD. To determine the molecular genetic basis for anthocyanin pigmentation in eggplant fruit, we used real-time polymerase chain reaction (PCR) to evaluate the expression of anthocyanin biosynthetic (Chs, Dfr, Ans) and regulatory (Myc, Myb(B), Myb(C), Wd) genes in S. melongena genotypes that produce fruit with dark violet ('Classic') or white ('Ghostbuster') coloration, respectively. Transcript levels and anthocyanin content were evaluated in fruit at various stages of development ranging from small post-anthesis fruit to full-sized marketable fruit. Anthocyanin content increased 9-fold in developing violet-colored 'Classic' fruit, whereas low but detectable concentrations were found in white 'Ghostbuster' fruit. Chs, Dfr, and Ans as well as Myb(C) and Myc transcript levels were significantly higher in 'Classic' in comparison with 'Ghostbuster' fruit at comparable stages of fruit development with greatest differences observed for Ans transcript levels. Myb(C) and Myc transcript levels increased in developing 'Classic' fruit coincident with increasing anthocyanin content. Myb(B) and Wd transcript levels were not coordinated with changes in biosynthetic transcript levels or anthocyanin concentration. C1 [Stommel, John R.; Dumm, Judith M.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Stommel, JR (reprint author), ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, Bldg 010A,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM john.stommel@ars.usda.gov NR 48 TC 1 Z9 1 U1 4 U2 18 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAR PY 2015 VL 140 IS 2 BP 129 EP 135 PG 7 WC Horticulture SC Agriculture GA CG4JA UT WOS:000353250200003 ER PT J AU Chen, CX Okie, WR AF Chen, Chunxian Okie, William R. TI Novel Peach Flower Types in a Segregating Population from 'Helen Borchers' SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article ID CAROTENOIDS; PLUM AB Several new peach (Prunus persica) flower types were discovered in an F-2 segregating population from an open-pollinated, non-showy-flowered F-1 seedling of 'Helen Borchers', a double-flowered ornamental cultivar. The novel flower types were white and red single-flowered, non-showy blooms, as well as double-flowered, non-showy red, pink, white, and yellow phenotypes. The double, non-showy flowers were very attractive, and resembled pom-pom chrysanthemums. Yellow flower color is unknown in peach. Flower type in the F-2 family segregated approximate to 3:l for non-showy (Sh_) vs. showy (shsh), for anthocyanin-present vs. anthocyanin-absent, and for pink (R_) vs. red (rr), independently. Flower petal number segregated at about 9:3:4 for classes single:semi-double:double. Although both parents were late flowering, the F-1 was not. The F-2 seedlings showed a wide range in time of flowering. Higher petal number was correlated with later bloom, although it is unclear whether this is due to linkage or developmental differences in the flowers with extra petals. These novel flower types might be useful as ornamentals, and for use in genetics and breeding studies. Microsatellite analysis of possible pollen donors revealed that 'Oldmixon Free', a non-showy-flowered peach cultivar, was likely the pollen parent of the F-1. C1 [Chen, Chunxian; Okie, William R.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Chen, CX (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM chunxian.chen@ars.usda.gov FU U.S. Department of Agriculture National Program of Plant Genetic Resources, Genomics and Genetic Improvement [6606-21000-004-006] FX The research was partly supported by the U.S. Department of Agriculture National Program of Plant Genetic Resources, Genomics and Genetic Improvement (Project no. 6606-21000-004-006). We thank Bryan Blackburn and Luke Quick for their assistance in maintaining the materials, and thank Drs. Bruce Wood and Tom Beckman for their critical review. This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 20 TC 0 Z9 0 U1 2 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAR PY 2015 VL 140 IS 2 BP 172 EP 177 PG 6 WC Horticulture SC Agriculture GA CG4JA UT WOS:000353250200008 ER PT J AU Shen, J Dirks, R Havey, MJ AF Shen, Jia Dirks, Rob Havey, Michael J. TI Diallel Crossing Among Doubled Haploids of Cucumber Reveals Significant Reciprocal-cross Differences SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE Cucumis sativus; cytoplasmic effects; heterosis; parent-of-origin effects ID TEOSINTE CYTOPLASMIC GENOMES; ABNORMAL GROWTH MUTANT; MITOCHONDRIAL GENOME; REPETITIVE DNAS; SATIVUS L.; ZEA-MAYS; MAIZE; GENE; PLANTS; REARRANGEMENTS AB Cytoplasmic effects on plant performance have been documented, but are not well understood. Cucumber (Cucumis sativus) is a useful plant for studying organellar effects on phenotypes because chloroplasts show maternal transmission and mitochondria paternal transmission. We produced doubled haploids (DH) from divergent cucumber populations, generated reciprocal hybrids in a diallel crossing scheme, measured fresh and dry weights of plants 22-30 days after planting seed, estimated combining abilities and heterosis for early plant growth, and assessed performance differences between reciprocal hybrids with identical nuclear genotypes. Across experiments, general and specific combining abilities and reciprocal effects, as well as their interactions with replicated experiments, were all highly significant (P < 0.001). Hybrids consistently out-performed parental lines with average heterosis over midparent values between 14% and 30%. A mitochondrial mutant (MSC3) showed negative effects when used as the male due to paternal transmission of mitochondria, but not as the female parent. Reciprocal hybrids among wild-type DH parents were identified that differed significantly (P = 0.032 to 0.001) for dry and fresh weights across experiments, indicating that cucumber breeders should evaluate both directions of crosses when producing hybrid cultivars. Reciprocal hybrids from DH cucumbers offer a unique opportunity to study biological factors contributing to significantly better performances, due to specific nuclear-cytoplasmic combinations and/or parent-of-origin effects in identical nuclear backgrounds. C1 [Shen, Jia; Havey, Michael J.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Shen, Jia] Nanjing Agr Univ, Coll Hort, Nanjing, Jiangsu, Peoples R China. [Dirks, Rob] Rijk Zwaan BV, NL-4793 RS Fijnaart, Netherlands. [Dirks, Rob] Univ Ghent, Fac Biosci Engn, B-9000 Ghent, Belgium. [Havey, Michael J.] USDA ARS, Madison, WI 53706 USA. RP Havey, MJ (reprint author), USDA ARS, Madison, WI 53706 USA. EM mjhavey@wisc.edu FU Nanjing (China) Agricultural University; USDA Specialty Crops Research Initiative (USA) [2011-51181-30661] FX We gratefully acknowledge the support of Nanjing (China) Agricultural University to JS and grant 2011-51181-30661 from the USDA Specialty Crops Research Initiative (USA). We thank Peter Crump of the University of Wisconsin for the SAS diallel-analysis program. NR 56 TC 1 Z9 1 U1 1 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAR PY 2015 VL 140 IS 2 BP 178 EP 182 PG 5 WC Horticulture SC Agriculture GA CG4JA UT WOS:000353250200009 ER PT J AU Porensky, LM Veblen, KE AF Porensky, Lauren M. Veblen, Kari E. TI Generation of Ecosystem Hotspots Using Short-Term Cattle Corrals in an African Savanna SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE fragmentation; grazing lawn; islands of fertility; laikipia; legacy effects; Ol Pejeta ID ABANDONED PASTORAL SETTLEMENTS; LARGE HERBIVORES; SEMIARID SAVANNA; LONG-TERM; ENVIRONMENTAL HETEROGENEITY; CENTENNIAL MOUNTAINS; SPECIES-DIVERSITY; WATERING POINTS; VEGETATION; LIVESTOCK AB Worldwide, many rangelands are managed for multiple uses, and it is increasingly important to identify livestock management practices that maximize rangeland productivity, biodiversity, and wildlife conservation. In sub-Saharan Africa, pastoralists and ranchers use temporary thorn-fence corrals ("bomas") to protect livestock at night. Traditional boma sites (used for months or years, then abandoned) develop into productive ecosystem hotspots ("glades") that attract diverse wildlife and persist for decades or even centuries. In central Kenya, livestock managers have recently begun using metal-fenced "mobile bomas," which are moved after only days or weeks. Although the assumption is that mobile boma sites will also develop into glades, whether or not this is true remains unclear. We used a broad-scale manipulative experiment to evaluate the ecosystem-level effects of mobile bomas used for 1 month. We also investigated impacts of initial boma density on glade development. We randomly assigned 12 plots to one of three density treatments: one boma, two bomas 200 m apart, or two bomas 100 m apart. Before the experiment and at 1, 6, 12, 18, and 32 months after boma abandonment, we sampled soil nutrients, foliar nutrients, plant communities, and wildlife use (via dung counts) within abandoned boma sites (experimental glades) and at paired reference sites (200 m away). After 18 months, surface soil nutrient concentrations in experimental glades were similar to those in traditionally formed glades. Experimental glade plant communities became dominated by a palatable, rhizomatous grass species, Cynodon plectostachyus. After 32 months, wildlife use by browsing and mixed feeding ungulates was 9 times higher in experimental glades than at paired reference sites. Boma density had few impacts on within-glade development patterns. These results demonstrate that by concentrating livestock in short-term corrals, managers can create ecosystem hotspots that increase functional heterogeneity, attract wildlife, and provide palatable forage for livestock. Published by Elsevier Inc. on behalf of Society for Range Management. C1 [Porensky, Lauren M.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Veblen, Kari E.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Veblen, Kari E.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. RP Porensky, LM (reprint author), USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM lauren.porensky@ars.usda.gov RI Veblen, Kari/D-1439-2012 OI Veblen, Kari/0000-0001-7523-3368 FU National Science Foundation (GRF) [DDIG 09-09539, LTREB DEB-08-16453]; University of California at Davis (Plant Sciences Dept Fellowships); University of California at Davis (Jastro-Shields Research Scholarship); University of California at Davis (Benjamin Madson Research Scholarship) FX Research was funded in part by the National Science Foundation (GRF and DDIG 09-09539 to L.M.P., LTREB DEB-08-16453 to T.P.Y.) and the University of California at Davis (Plant Sciences Dept Fellowships, Jastro-Shields and Benjamin Madson Research Scholarships). NR 60 TC 5 Z9 5 U1 4 U2 28 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2015 VL 68 IS 2 BP 131 EP 141 DI 10.1016/j.rama.2015.01.002 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CG3ZA UT WOS:000353217600003 ER PT J AU Thomas, MG Mohamed, AH Sawalhah, MN Holechek, JL Bailey, DW Hawkes, JM Luna-Nevarez, P Molinar, F Khumalo, G AF Thomas, Milton G. Mohamed, Ahmed H. Sawalhah, Mohammed N. Holechek, Jerry L. Bailey, Derek W. Hawkes, Jerry M. Luna-Nevarez, Pablo Molinar, Francisco Khumalo, Godfrey TI Long-Term Forage and Cow-Calf Performance and Economic Considerations of Two Stocking Levels on Chihuahuan Desert Rangeland SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Cattle productivity; Drought; Forage; Grazing management; Rangelands ID REPRODUCTIVE-PERFORMANCE; CLIMATE-CHANGE; BRANGUS COWS; CATTLE; TRENDS; VARIABILITY; MODERATE; BREED AB Forage and cow-calf productivity on two lightly and two conservatively grazed pastures were evaluated over a 15-year period (1997-2011) in the Chihuahuan Desert of south-central New Mexico. Spring-calving Brangus cows were randomly assigned to pastures in January of each year. Pastures were similar in area (1 098 +/- 69 ha, mean +/- SE) with similar terrain and distance towater. Utilization of primary forage species averaged 27.1 +/- 3.0% in lightly stocked pastures and 39.4 +/- 4.0% on conservatively stocked pastures. No differences in perennial grass standing crop (163.5 +/- 52.2 kg.ha(-1)) and calf weaning weights (286.1 +/- 2.6 kg) were detected (P > 0.10) between light and conservative treatments. Lightly grazed pastures yielded greater (P < 0.05) kg of calf weaned.ha(-1) and calf crop percent than conservatively grazed pastures in 1998 due to complete destocking of conservatively grazed pastures during that slight drought (i.e., rainfall was 75% of normal in 1998). After the initial 5 years of study (1997-2001), all pastures were destocked for 4 years (2002-2005) due to drought as rainfall was only 50% or less of normal. Pastures were then restocked for another 6 years (2006-2011). Postdrought, the percentage change in perennial grass standing forage crop (kg.ha(-1)) was -4.0 and -14.4 +/- 2.5 % (P < 0.09) in the light and conservative grazed pastures across the 6 years, respectively. While conservative stocking rates may provide higher net financial returns than light stocking rates during nondrought years as there were more AU per pasture, potential losses from cattle liquidation during short-term (i.e., 1-year) droughts could nullify this advantage. Results suggest that light grazing use of forage is a practical approach for Chihuahuan Desert cow-calf operations to minimize risk of herd liquidation during short-term drought. Published by Elsevier Inc. on behalf of Society for Range Management. C1 [Thomas, Milton G.] Colorado State Univ, Dept Anim Sci, Beef Cattle Breeding & Genet, Ft Collins, CO 80523 USA. [Mohamed, Ahmed H.] Desert Res Ctr, Dept Plant Ecol & Range Management, Cairo, Egypt. [Sawalhah, Mohammed N.] New Mexico State Univ, Las Cruces, NM 88003 USA. [Holechek, Jerry L.; Bailey, Derek W.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. [Luna-Nevarez, Pablo] Inst Technol De Sonora, Dept Ciencias Agron & Vet, Obregon 85000, Son, Mexico. [Molinar, Francisco] USDA, Nat Resource Conservat Serv, El Paso, TX 79936 USA. [Khumalo, Godfrey] Univ Swaziland, Anim Sci, Luyengo MR18, Swaziland. RP Holechek, JL (reprint author), New Mexico State Univ, Dept Anim & Range Sci, 2980 South Espina St,MSC 3I, Las Cruces, NM 88003 USA. EM holechek@nmsu.edu FU New Mexico Agricultural Experiment Station [072944] FX Research supported by New Mexico Agricultural Experiment Station (project 072944). NR 54 TC 1 Z9 1 U1 2 U2 5 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2015 VL 68 IS 2 BP 158 EP 165 DI 10.1016/j.rama.2015.01.003 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CG3ZA UT WOS:000353217600006 ER PT J AU Nafus, AM Svejcar, TJ Ganskopp, DC Davies, KW AF Nafus, Aleta M. Svejcar, Tony J. Ganskopp, David C. Davies, Kirk W. TI Abundances of Coplanted Native Bunchgrasses and Crested Wheatgrass after 13 Years SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Agropyron cristatum; restoration; revegetation; sagebrush steppe ID GREAT-BASIN; AGROPYRON-CRISTATUM; SEED BANKS; GRASS; INVASION; VEGETATION; RANGELANDS; DIVERSITY; IMPACTS; PRAIRIE AB Crested wheatgrass (Agropyron cristatum [L] Gaertm) has been seeded on more than 5 million hectares in western North America because it establishes more readily than native bunchgrasses. Currently, there is substantial interest in reestablishing native species in sagebrush steppe, but efforts to reintroduce native grasses into crested wheatgrass stands have been largely unsuccessful, and little is known about the long-term dynamics of crested wheatgrass/native species mixes. We examined the abundance of crested wheatgrass and seven native sagebrush steppe bunchgrasses planted concurrently at equal low densities in nongrazed and unburned plots. Thirteen years post establishment, crested wheatgrass was the dominant bunchgrass, with a 10-fold increase in density. Idaho fescue (Festuca idahoensis Elmer), Thurber's needlegrass (Achnatherum thurberianum (Piper) Barkworth), basin wildrye (Leymus cinereus [Scribn. & Merr.] A. Love), and Sandberg bluegrass (Poa secunda J. Presl) maintained their low planting density, whereas bluebunch wheatgrass (Pseudoroegneria spicata [Pursh] A. Love), needle-and-thread (Hesperostipa comata [Trin. & Rupr.] Barkworth), and squirreltail (Elymus elymoides [Raf.] Swezey) densities declined. Our results suggest that densities of native bunchgrasses planted with crested wheatgrass are unlikely to increase and that some species may only persist at low levels. The high recruitment of crested wheatgrass suggests that coplanting of some native bunchgrasses may be a viable way of avoiding crested wheatgrass monocultures when this species is necessary for rehabilitation or restoration. (C) 2015 Society for Range Management. Published by Elsevier Inc. All rights reserved. C1 [Nafus, Aleta M.] Oregon State Univ, Corvallis, OR 97330 USA. [Nafus, Aleta M.; Svejcar, Tony J.; Ganskopp, David C.; Davies, Kirk W.] USDA ARS, Burns, OR 97720 USA. RP Nafus, AM (reprint author), USDA ARS, 67826 A Hwy 205, Burns, OR 97720 USA. EM aleta.nafus@oregonstate.edu FU Bureau of Land Management Great Basin Native Plant Selection and Increase Project; USDA-Agricultural Research Service; Oregon Dept of Fish and Wildlife FX Research was funded in part by the Bureau of Land Management Great Basin Native Plant Selection and Increase Project with additional support provided by the USDA-Agricultural Research Service and the Oregon Dept of Fish and Wildlife. NR 30 TC 4 Z9 4 U1 3 U2 21 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2015 VL 68 IS 2 BP 211 EP 214 DI 10.1016/j.rama.2015.01.011 PG 4 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CG3ZA UT WOS:000353217600012 ER PT J AU Fisher, JR Fisher, DM Nelson, WA O'Neill, JC Skvarla, MJ Ochoa, R Bauchan, GR Radwell, AJ Dowling, APG AF Fisher, J. Ray Fisher, Danielle M. Nelson, Whitney A. O'Neill, Joseph C. Skvarla, Michael J. Ochoa, Ron Bauchan, Gary R. Radwell, Andrea J. Dowling, Ashley P. G. TI Torrenticola trimaculata n. sp (Parasitengona: Torrenticolidae), a three-spotted water mite from eastern North America: taxonomic history, species delimitation, and survey of external morphology SO ACAROLOGIA LA English DT Article DE Trombidiformes; Prostigmata; Hydrachnidia; Hydrachnidiae; LT-SEM; Cocconeidaceae ID ACARI HYDRACHNIDIA TORRENTICOLIDAE; SUCTORIAN CILIATES CILIOPHORA; MONATRACTIDES K. VIETS; PALEARCTIC REGION; 1896 ACARIFORMES; PIERSIG; HYDRACHNELLAE; LEBERTIOIDEA; PROSTIGMATA; FAMILY AB Torrenticola trimaculata Fisher n. sp. is described from eastern North America as the first in a series of descriptions on Torrenticolidae. As such, the study includes expanded discussions of methods, early taxonomic history, and numerous images surveying external morphology using a diversity of imaging methods. Species hypotheses were supported with analysis of the ''barcoding" region of COI. Torrenticola trimaculata is found to be a wide-ranging, variable species with two distinct morphs that do not coexist locally. Also, we report the first record of the diatom, Cocconeis placentula Ehrenberg 1838, as epiphytic on water mites. C1 [Fisher, J. Ray; Fisher, Danielle M.; Nelson, Whitney A.; O'Neill, Joseph C.; Skvarla, Michael J.; Radwell, Andrea J.; Dowling, Ashley P. G.] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. [Ochoa, Ron; Bauchan, Gary R.] USDA ARS, BARC West, Beltsville, MD 20705 USA. RP Fisher, JR (reprint author), Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. EM jrfisher@uark.edu; dkeeler@uark.edu; whitneyanelson@gmail.com; joneill@email.uark.edu; mskvarla36@gmail.com; ron.ochoa@ars.usda.gov; gary.bauchan@ars.usda.gov; radwell33@gmail.com; adowling@uark.edu FU National Science Foundation [DEB 1134868] FX We thank Ian Smith (CNC) for his expertise throughout the project and helpful comments reviewing the manuscript; Andy Alverson (University of Arkansas) for identifying the epiphytic diatoms; Reinhard Gerecke for helpful discussions of taxonomic history; USDA for use of LT-SEM; CNC for slide material; and our friends and families that support us all. This material is based upon work supported by the National Science Foundation under Grant No. DEB 1134868. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer. NR 180 TC 2 Z9 2 U1 2 U2 7 PU ACAROLOGIA-UNIVERSITE PAUL VALERY PI MONTPELLIER PA SERVICES PUBLICATIONS, 17 REU ABBE DE L EPEE, 34090 MONTPELLIER, FRANCE SN 0044-586X EI 2107-7207 J9 ACAROLOGIA JI Acarologia PD MAR PY 2015 VL 55 IS 1 BP 71 EP 116 DI 10.1051/acarologia/20152155 PG 46 WC Entomology SC Entomology GA CF9XH UT WOS:000352920000008 ER PT J AU Muhammad, A AF Muhammad, Andrew TI Price Risk and Exporter Competition in China's Soybean Market SO AGRIBUSINESS LA English DT Article ID EXCHANGE-RATE UNCERTAINTY; AGRICULTURAL TRADE; DEMAND; VOLATILITY; MODEL AB An import allocation model is used to examine the effects of price risk (variance of prices) on exporter competition in China's soybean market. Price risk is an important determinant of China's soybean imports across sources (Argentina, Brazil, and the United States), even when accounting for other factors. Results indicate that Argentina is the only country affected by own-price risk in the Chinese market; imports decline by 1.11% for every percentage increase price risk. The estimated risk premium for soybeans from Argentina is 0.44, indicating that if price risk increases by 1%, prices would have to fall by 0.44% for imports to remain unchanged. Price risk in Argentina has a positive effect on China's imports from the United States. Price risk in Brazil has a positive effect on imports from Argentina, but a negative effect on imports from the United States. [EconLit citations: D81, F14, Q11, Q17]. C1 [Muhammad, Andrew] USDA, Int Demand & Trade Branch, Market & Trade Econ Div, Econ Res Serv, Washington, DC 20024 USA. [Muhammad, Andrew] USDA, Econ Res Serv, Washington, DC USA. RP Muhammad, A (reprint author), USDA, Int Demand & Trade Branch, Market & Trade Econ Div, Econ Res Serv, Washington, DC 20024 USA. EM amuhammad@ers.usda.gov NR 24 TC 1 Z9 1 U1 3 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0742-4477 EI 1520-6297 J9 AGRIBUSINESS JI Agribusiness PD SPR PY 2015 VL 31 IS 2 BP 188 EP 197 DI 10.1002/agr.21393 PG 10 WC Agricultural Economics & Policy; Economics; Food Science & Technology SC Agriculture; Business & Economics; Food Science & Technology GA CF8FN UT WOS:000352791600003 ER PT J AU Logan-Young, CJ Yu, JZ Verma, SK Percy, RG Pepper, AE AF Logan-Young, Carla Jo Yu, John Z. Verma, Surender K. Percy, Richard G. Pepper, Alan E. TI SNP DISCOVERY IN COMPLEX ALLOTETRAPLOID GENOMES (GOSSYPIUM SPP., MALVACEAE) USING GENOTYPING BY SEQUENCING SO APPLICATIONS IN PLANT SCIENCES LA English DT Article DE genotyping by sequencing; Gossypium; interspecific; intraspecific; next-generation sequencing; polyploid; single-nucleotide polymorphisms ID COTTON; PLANT; HIRSUTUM; IDENTIFICATION; EVOLUTION; RESOURCE; REPEAT; L. AB Premise of the study: Single-nucleotide polymorphism (SNP) marker discovery in plants with complex allotetraploid genomes is often confounded by the presence of homeologous loci (along with paralogous and orthologous loci). Here we present a strategy to filter for SNPs representing orthologous loci. Methods and Results: Using Illumina next-generation sequencing, 54 million reads were collected from restriction enzyme-digested DNA libraries of a diversity of Gossypium taxa. Loci with one to three SNPs were discovered using the Stacks software package, yielding 25,529 new cotton SNP combinations, including those that are polymorphic at both interspecific and intraspecific levels. Frequencies of predicted dual-homozygous (aa/bb) marker polymorphisms ranged from 6.7-11.6% of total shared fragments in intraspecific comparisons and from 15.0-16.4% in interspecific comparisons. Conclusions: This resource provides dual-homozygous (aa/bb) marker polymorphisms. Both in silico and experimental validation efforts demonstrated that these markers are enriched for single orthologous loci that are homozygous for alternative alleles. C1 [Logan-Young, Carla Jo; Verma, Surender K.; Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Yu, John Z.; Percy, Richard G.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Pepper, AE (reprint author), Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. EM apepper@bio.tamu.edu FU Cotton Incorporated [08-380]; CREST Award from the Ministry of Science and Technology of India; [3091-21000-038-03] FX We would like to express our deepest gratitude to James Frelichowski and Jared Harris for their help with cotton germplasm resources. This work was funded by Cotton Incorporated Fellowship 08-380 (to C.J.L.Y.) and specific cooperative agreement 3091-21000-038-03 (A.E.P. and J.Z.Y.). S.K.V. was supported by a CREST Award from the Ministry of Science and Technology of India. NR 26 TC 6 Z9 6 U1 7 U2 27 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 2168-0450 J9 APPL PLANT SCI JI Appl. Plant Sci. PD MAR PY 2015 VL 3 IS 3 AR 1400077 DI 10.3732/apps.1400077 PG 8 WC Plant Sciences SC Plant Sciences GA CG1SD UT WOS:000353053300001 ER PT J AU Rogers, EE Ledbetter, CA AF Rogers, Elizabeth E. Ledbetter, Craig A. TI Susceptibility to Xylella fastidiosa in a First-generation Hybrid from a non-traditional Peach-Almond Cross SO HORTSCIENCE LA English DT Article DE Prunus persica; Prunus dulcis; Prunus webbii; interspecific hybrid; rootstock; titer ID LEAF SCORCH DISEASE; SUBSP MULTIPLEX; PHONY DISEASE; STRAINS; CALIFORNIA; RESISTANCE; DIVERSITY; ETIOLOGY; CITRUS; SCALD AB To facilitate development of Prunus L. rootstocks with desirable agronomic traits, domesticated peach (Prunus persica) and almond (P. dulcis) were crossed with wild almond relatives. This work reports that a hybrid from a P. webbii x P. persica cv. Harrow Blood cross is susceptible to almond leaf scorch disease (ALSD). ALSD is caused by the fastidious, xylem-limited bacterium Xylella fastidiosa. The P. webbii X 'Harrow Blood' hybrid, along with its parents, was inoculated with two ALSD-inducing strains (X. fastidiosa subsp. fastidiosa strain M23 and subsp. multiplex strain Dixon). Both X. fastidiosa strains grew to high titer in the susceptible P. webbii parent and in the interspecific hybrid; defoliation was also observed. As expected, 'Harrow Blood' did not exhibit defoliation symptoms or support growth of X. fastidiosa. This result contrasts with earlier work demonstrating that a P. persica x P. dulcis hybrid is not a suitable host for X. fastidiosa subsp. fastidiosa M23. It appears that the genetic basis of resistance/susceptibility differs between a P. persica x P. dulcis cross and the P. webbii X P. persica cross reported here. Understanding the degree of susceptibility to X. fastidiosa in complex hybrids of subgenus Amygdalus should be an important part of rootstock development. C1 [Rogers, Elizabeth E.; Ledbetter, Craig A.] ARS, USDA, San Joaquin Agr Sci Ctr, Parlier, CA 93648 USA. RP Rogers, EE (reprint author), ARS, USDA, San Joaquin Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM elizabeth.rogers@ars.usda.gov RI Rogers, Elizabeth/D-2087-2009 OI Rogers, Elizabeth/0000-0002-0545-4744 NR 31 TC 1 Z9 1 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2015 VL 50 IS 3 BP 337 EP 340 PG 4 WC Horticulture SC Agriculture GA CF7RM UT WOS:000352754000002 ER PT J AU Goenaga, R Guiltinan, M Maximova, S Seguine, E Irizarry, H AF Goenaga, Ricardo Guiltinan, Mark Maximova, Siela Seguine, Ed Irizarry, Heber TI Yield Performance and Bean Quality Traits of Cacao Propagated by Grafting and Somatic Embryo-derived Cuttings SO HORTSCIENCE LA English DT Article DE micropropagation; cocoa; quality ID THEOBROMA-CACAO; L.; EMBRYOGENESIS; VARIABILITY AB Twelve cacao (Theobroma cacao) clones propagated by grafting and orthotropic rooted cuttings of somatic embryo-derived plants were grown on an Ultisol soil at Corozal, Puerto Rico, and evaluated for 6 years of production under intensive management. Year, variety, year x variety, and propagation treatment x variety interactions indicated significant effects for dry bean yield, number of pods produced, pod index, plant height, and stem diameter. Propagation treatments had a significant effect on dry bean yield and pod index but not on number of pods produced. Average yield across varieties for both propagation treatments was 2087.9 kg.ha(-1) per year of dry beans. There was a highly significant variety effect. 'UF-668' was the top yielder averaging 2536.7 kg.ha(-1) per year of dry beans; however, this yield was not significantly different from the average yield of varieties 'TARS-30', 'TARS-1', 'TARS-13', 'TARS-14', and 'TARS-2', which averaged 2427.0 kg.ha(-1) per year. Except for 'UF-668', the TARS varieties were released in 2009 as high-yielding selections. Propagation treatments had a significant effect on dry bean yield. Dry bean yield of varieties propagated by grafting was 7% higher (2166.7 kg.ha(-1) per year) than those propagated by orthotropic rooted cuttings of somatic embryo-derived plants (2009.2 kg.ha(-1) per year). This yield difference could not be attributed to grafted plants being more vigorous nor by differences in root architecture. The lowest pod index value in both propagation treatments was obtained by 'UF-668'; however, pod index for this variety did not differ significantly from values for 'TARS-2' and 'TARS-23' in grafted plants and from 'TARS-2', 'TARS-23', and 'TARS-1' in plants propagated by orthotropic rooted cuttings of somatic embryo-derived plants. With few exceptions, flavor characteristics were not significantly affected by propagation treatments. Although there were significant differences between plant propagation treatments for some of the variables measured in this study, these were not of a magnitude that would preclude the use of somatic embryogenesis as a viable propagation system for cacao. C1 [Goenaga, Ricardo; Irizarry, Heber] ARS, Trop Agr Res Stn, USDA, Mayaguez, PR 00680 USA. [Guiltinan, Mark; Maximova, Siela] Penn State Univ, Dept Hort, University Pk, PA 16802 USA. [Seguine, Ed] MARS Inc, Hanover, PA 17331 USA. RP Goenaga, R (reprint author), ARS, Trop Agr Res Stn, USDA, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. EM ricardo.goenaga@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2015 VL 50 IS 3 BP 358 EP 362 PG 5 WC Horticulture SC Agriculture GA CF7RM UT WOS:000352754000005 ER PT J AU Baldos, OC DeFrank, J Sakamoto, GS AF Baldos, Orville C. DeFrank, Joseph Sakamoto, Glenn S. TI Germination Response of Dormant Tanglehead (Heteropogon contortus) Seeds to Smoke-infused Water and the Smoke-associated Stimulatory Compounds, Karrikinolide and Cyanide SO HORTSCIENCE LA English DT Article DE piligrass; speargrass; potassium cyanide; benzaldehyde; mandelonitrile; food-grade liquid smoke; xylose smoke-infused water ID PLANT-DERIVED SMOKE; TERSONIA-CYATHIFLORA GYROSTEMONACEAE; SEEDLING GROWTH; WOOD SMOKE; RED RICE; LYCOPERSICON-ESCULENTUM; BURNING VEGETATION; LIQUID SMOKE; BUTENOLIDE; PROMOTES AB Tanglehead (Heteropogon contortus) is a native Hawaiian grass that has been used in restoration and has potential for expanded re-vegetation use. Although interest and demand for tanglehead re-vegetation has increased, the supply of tanglehead seeds has remained limited as a result of a lack of seed production protocols addressing seed dormancy. Smoke water from burning vegetation may provide an economical and practical seed treatment because aerosol smoke has been reported to stimulate tanglehead seed germination. Dose rate and side-by-side comparison studies were conducted to evaluate the germination stimulation efficacy of food-grade liquid smoke, xylose smoke-infused water, tanglehead smoke-infused water, karrikinolide (KARD, and cyanide (i.e., mandelonitrile and potassium cyanide). Optimum smoke water dilutions were 1% v/v for food-grade liquid smoke and undiluted for xylose smoke-infused water and tanglehead smoke-infused water. KAR(1) was not stimulatory at concentrations between 0.0067 and 66.7 mu m. Potassium cyanide stimulated tanglehead seed germination at concentrations between 50 to 500 mu m. Germination was promoted to even greater levels with the cyanohydrin, mandelonitrile, indicating a role for benzaldehyde (a byproduct of mandelonitrile decomposition) in stimulating tanglehead seed germination. Benzaldehyde was confirmed to be stimulatory at concentrations between 50 to 100 mu M. The presence of cyanide at stimulatory levels was confirmed in tanglehead smoke-infused water (i.e., approximate to 100 mu m), but not in food-grade liquid smoke or xylose smoke-infused water. Germination with non-cyanide-containing smoke waters indicates the presence of other compounds in smoke that can stimulate tanglehead germination. In the side-by-side comparison study, food-grade liquid smoke (1% v/v) and undiluted tanglehead smoke-infused water provided consistent germination stimulation comparable to 500 mu M potassium cyanide. Undiluted xylose smoke-infused water did not provide significant germination stimulation in the comparison studies. This may be the result of differences in seed batch sensitivity to the germination stimulant, seed storage duration as well as subtle differences in the preparation of xylose smoke-infused water. C1 [Baldos, Orville C.; DeFrank, Joseph] Univ Hawaii Manoa, Dept Trop Plant & Soil Sci, St John Plant Sci Lab 102, Honolulu, HI 96822 USA. [Sakamoto, Glenn S.] Nat Resources Conservat Serv, USDA, Hoolehua Plant Mat Ctr, Hoolehua, HI 96729 USA. RP Baldos, OC (reprint author), Univ Hawaii Manoa, Dept Trop Plant & Soil Sci, St John Plant Sci Lab 102, 3190 Maile Way, Honolulu, HI 96822 USA. EM obaldos@hawaii.edu NR 66 TC 0 Z9 0 U1 3 U2 17 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2015 VL 50 IS 3 BP 421 EP 429 PG 9 WC Horticulture SC Agriculture GA CF7RM UT WOS:000352754000016 ER PT J AU Vargas, OL Bryla, DR AF Vargas, Oscar L. Bryla, David R. TI Growth and Fruit Production of Highbush Blueberry Fertilized with Ammonium Sulfate and Urea Applied by Fertigation or as Granular Fertilizer SO HORTSCIENCE LA English DT Article DE Vaccinium corymbosum; fertilizer practices; leaf nutrients; nitrogen management; salinity; soil pH ID NITROGEN; ESTABLISHMENT; NITRATE; YIELD; PH AB Fertigation with liquid sources of nitrogen (N) fertilizers, including ammonium sulfate and urea, were compared with granular applications of the fertilizers in northern highbush blueberry (Vaccinium corymbosum L. 'Bluecrop') during the first 5 years of fruit production (2008-12). The planting was established in Apr. 2006 at a field site located in western Oregon. The plants were grown on raised beds and mulched every 2 years with sawdust. Liquid fertilizers were injected through a drip system in equal weekly applications from mid-April to early August. Granular fertilizers were applied on each side of the plants, in three split applications from mid-April to mid-June, and washed into the soil using microsprinklers. Each fertilizer was applied at three N rates, which were increased each year as the plants matured (63 to 93, 133 to 187, and 200 to 280 kg.ha(-1) N) and compared with non-fertilized treatments (0 kg.ha(-1) N). Canopy cover, which was measured in 2008 only, and fresh pruning weight were greater with fertigation than with granular fertilizer and often increased with N rate when the plants were fertigated but decreased at the highest rate when granular fertilizer was applied. Yield also increased with N fertilizer and was 12% to 40% greater with fertigation than with granular fertilizer each year as well as 17% greater with ammonium sulfate than with urea in 2011. The response of berry weight to the treatments was variable but decreased with higher N rates during the first 3 years of fruit production. Leaf N concentration was greater with fertigation in 4 of 5 years and averaged 1.68% with fertigation and 1.61% with granular fertilizer. Leaf N was also often greater with ammonium sulfate than with urea and increased as more N was applied. Soil pH declined with increasing N rates and was lower with granular fertilizer than with fertigation during the first 3 years of fruit production and lower with ammonium sulfate than with urea in every year but 2010. Soil electrical conductivity (EC) was less than 1 dS.m(-1) in each treatment but was an average of two to three times greater with granular fertilizer than with fertigation and 1.4 to 1.8 times greater with ammonium sulfate than with urea. Overall, total yield averaged 32 to 63 t.ha(-1) in each treatment over the first 5 years of fruit production and was greatest when plants were fertigated with ammonium sulfate or urea at rates of at least 63 to 93 kg.ha(-1) N per year. C1 [Vargas, Oscar L.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Bryla, David R.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Bryla, DR (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM david.bryla@ars.usda.gov FU Oregon Blueberry Commission; Northwest Center for Small Fruits Research FX Financial support was provided by the Oregon Blueberry Commission, the Northwest Center for Small Fruits Research and our industry contributors. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 37 TC 3 Z9 3 U1 4 U2 28 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2015 VL 50 IS 3 BP 479 EP 485 PG 7 WC Horticulture SC Agriculture GA CF7RM UT WOS:000352754000025 ER PT J AU Ehret, DL Frey, B Forge, T Helmer, T Bryla, DR AF Ehret, David L. Frey, Brenda Forge, Tom Helmer, Tom Bryla, David R. TI Age-related Changes in Response of Highbush Blueberry Plants to Drip Irrigation SO HORTSCIENCE LA English DT Article DE Vaccinium corymbosum; antioxidant; fruit firmness; fruit size; nutritional health benefit; soil water potential ID WATER REQUIREMENTS; FRUIT-QUALITY; YIELD AB A study was conducted in the Fraser Valley of British Columbia, Canada, to determine the effects of drip configuration (one or two lines with emitters spaced every 0.3 or 0.45 m) and irrigation at moderate or heavy rates (5 or 10 L/plant) in a mature planting of 'Duke' highbush blueberry (Vaccinium corymbosum L.). Results were compared with those published previously from the first 4 years after planting. Although plant size increased with irrigation rate when the plants were younger, there was no added benefit of heavy irrigation on growth in the older plants. However, the plants became more sensitive to soil water deficits with age and, therefore, unlike when they were younger, had greater yields when more water was applied. Berry size and fruit firmness were little affected by irrigation in the older plants, but antioxidants, measured as oxygen radical absorbance capacity (ORAC), were higher with than without irrigation, suggesting that irrigation has the potential to improve the health benefits of blueberries. Growth, yield, and fruit quality were unaffected by drip configuration in any year. Overall, the results revealed that the response of highbush blueberry to drip irrigation changed over time and indicated that irrigation management should be adjusted as a planting matures. C1 [Ehret, David L.; Frey, Brenda; Helmer, Tom] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Forge, Tom] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC V0H 1Z0, Canada. [Bryla, David R.] ARS, USDA, Hort Crop Res Unit, Corvallis, OR 97330 USA. RP Ehret, DL (reprint author), Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. EM david.ehret@agr.gc.ca FU BC Blueberry Council; AAFC Developing Innovative Agri-Products (DIAP) program FX We are grateful to the BC Blueberry Council and the AAFC Developing Innovative Agri-Products (DIAP) program for financial assistance; to Elyse Hofs, Karlee Haak, and June Dawson for plot work; and to Mark Sweeney for horticultural advice. NR 14 TC 1 Z9 1 U1 1 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2015 VL 50 IS 3 BP 486 EP 490 PG 5 WC Horticulture SC Agriculture GA CF7RM UT WOS:000352754000026 ER PT J AU Zhang, FM Chen, JM Pan, YD Birdsey, RA Shen, SH Ju, WM Dugan, AJ AF Zhang, Fangmin Chen, Jing M. Pan, Yude Birdsey, Richard A. Shen, Shuanghe Ju, Weimin Dugan, Alexa J. TI Impacts of inadequate historical disturbance data in the early twentieth century on modeling recent carbon dynamics (1951-2010) in conterminous US forests SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE U; S; forests; carbon sink; disequilibrium state; stand age; disturbance ID CLIMATE-CHANGE; CANADA FORESTS; TEMPORAL PATTERNS; BALANCE; CO2; EQUILIBRIUM; TEMPERATURE; VEGETATION; STORAGE; FUTURE AB Stand age and disturbance data have become more available in recent years and can facilitate modeling studies that integrate and quantify effects of disturbance and nondisturbance factors on carbon dynamics. Since high-quality disturbance and forest age data to support forest dynamic modeling are lacking before 1950, we assumed dynamic equilibrium (carbon neutrality) for the starting conditions of forests with unknown historical disturbance and forest age information. The impacts of this assumption on forest carbon cycle estimation for recent decades have not been systematically examined. In this study, we tested an assumption of disequilibrium conditions for forests with unknown disturbance and age data by randomly assigning ages to them in the model initial year (1900) and analyzed uncertainties for 1951-2010 carbon dynamic simulations compared with the equilibrium assumption. Results show that with the dynamic equilibrium assumption, the total net biome productivity (NBP) of conterminous U.S. forests was 18860TgCyr(-1) with 18556TgCyr(-1) in living biomass and 323TgCyr(-1) in soil. The C release due to disturbance on average was about 6855TgCyr(-1). The disequilibrium assumption causes annual NBP from 1951 to 2010 in conterminous U.S. forests to vary by an average of 13% with the largest impact on the soil carbon component. Uncertainties related to nondisturbance factors have relatively small impacts on NBP estimation (within 10%), while uncertainties related to disturbances cause biases in NBP of 4 to 28%. We conclude that the dynamic equilibrium assumption for the model initialization in 1900 is acceptable for simulating 1951-2010 forest carbon dynamics as long as disturbance and age data are reliable for this later period, although caution should be taken regarding the prior-1950 simulation results because of their greater uncertainties. Key Points C1 [Zhang, Fangmin; Shen, Shuanghe] Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Collaborat Innovat Ctr Forecast & Evaluat Meteoro, Nanjing, Jiangsu, Peoples R China. [Zhang, Fangmin; Chen, Jing M.] Univ Toronto, Dept Geog, Toronto, ON M5S 1A1, Canada. [Pan, Yude; Birdsey, Richard A.; Dugan, Alexa J.] US Forest Serv, USDA, Marienville, PA USA. [Ju, Weimin] Nanjing Univ, Int Inst Earth Syst Sci, Nanjing 210008, Jiangsu, Peoples R China. RP Zhang, FM (reprint author), Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Collaborat Innovat Ctr Forecast & Evaluat Meteoro, Nanjing, Jiangsu, Peoples R China. EM fmin.zhang@utoronto.ca RI Pan, Yude/F-6145-2015 FU National Natural Science Fund for Young Scholar [31300420, 41105078, BK20130987]; Jiangsu Natural Science Fund for Young Scholar [31300420, 41105078, BK20130987]; USDA Forest Service Research grant [07-JV-11242300-114] FX We greatly appreciate the availability of the annual tower flux data from the AmeriFlux network sites. We are greatly indebted to the principle investigators of AmeriFlux and their research teams operating the 35 forest sites selected for our model validation. Thanks are also extended to Eric Sundquist and two anonymous reviewers for providing constructive comments during the review process. The research is supported by a research grant from National and Jiangsu Natural Science Funds for Young Scholar (31300420, 41105078, and BK20130987) and USDA Forest Service Research grant (07-JV-11242300-114). Data and models to support this article are from the research collaboration of Jingming Chen at University of Toronto with the U.S. Forest Service. Please contact fmin.zhang@utoronto.ca for additional information about this study. NR 56 TC 3 Z9 3 U1 2 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD MAR PY 2015 VL 120 IS 3 BP 549 EP 569 DI 10.1002/2014JG002798 PG 21 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CG1PT UT WOS:000353046200012 ER PT J AU Carnohan, LP Kaufman, PE Allan, SA Gezan, SA Weeks, ENI AF Carnohan, Lucas P. Kaufman, Phillip E. Allan, Sandra A. Gezan, Salvador A. Weeks, Emma N. I. TI Evaluation of Four Bed Bug Traps for Surveillance of the Brown Dog Tick (Acari: Ixodidae) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Rhipicephalus sanguineus; semiochemical; monitoring; carbon dioxide; heat ID RHIPICEPHALUS-SANGUINEUS LATREILLE; CIMEX-LECTULARIUS; CHEMO-ATTRACTION; CARBON-DIOXIDE; ECOLOGY; BRAZIL AB The brown dog tick, Rhipicephalus sanguineus (Latrielle), can be a serious residential pest due to its unique ability, among ticks, to complete its lifecycle indoors. A single engorged and fertilized female tick can oviposit around 4,000 eggs, allowing indoor establishment to be rapid and easy to miss in early-stage infestations. Acaricide treatment is currently the primary method of control, but can be costly and can lead to the development of acaricide resistance in tick populations. Traps of various designs can be used to help monitor and manage populations of indoor pests, such as cockroaches and bed bugs, but there are currently no commercially available traps for use with brown dog tick infestations. This study included a comparison of four commercially available bed bug traps (NightWatch [BioSensory Inc., Putnam, CT], Bed Bug Beacon [PackTite, Fort Collins, CO], ClimbUp [Susan McKnight Inc., Memphis, TN], and Verify [FMC Corporation, Philadelphia, PA]) with regard to their efficacy in capturing brown dog ticks, and also compared tick attraction to ClimbUp traps baited with several stimuli including CO2. Significantly more ticks were captured and attracted to the NightWatch and CO2-baited ClimbUp traps than the other two trap models. The results suggest that bed bug traps may be useful in brown dog tick monitoring, and CO2 will likely be an important component of a trapping system employed in the future. C1 [Carnohan, Lucas P.; Kaufman, Phillip E.; Weeks, Emma N. I.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Allan, Sandra A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Gezan, Salvador A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. RP Weeks, ENI (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM eniweeks@ufl.edu FU USDA; National Institute of Food and Agriculture (NIFA); Pest Management Alternatives Grants Program [FLA-ENY-005055]; University of Florida, Institute of Food and Agricultural Sciences Dean's Matching Assistantship Program FX We would like to thank Lois Wood for her help in the laboratory, and Karen Prine, Tim Davis, Amanda Eiden, and Chris Holderman for their assistance with preparing this article. Also a special thanks to Eric Paulsen and Albert Pons (United States Department of Agriculture [USDA]) who assisted with the construction of the mesocosms. Funding was provided by the USDA, National Institute of Food and Agriculture (NIFA), Pest Management Alternatives Grants Program through project FLA-ENY-005055 and the University of Florida, Institute of Food and Agricultural Sciences Dean's Matching Assistantship Program. NR 28 TC 1 Z9 1 U1 4 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2015 VL 52 IS 2 BP 260 EP 268 DI 10.1093/jme/tju020 PG 9 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CF2UE UT WOS:000352402300018 PM 26336310 ER PT J AU Prusinski, MA Mertins, JW Meehan, LJ AF Prusinski, M. A. Mertins, J. W. Meehan, L. J. TI Two Gynandromorphs of Ixodes scapularis (Acari: Ixodidae) from New York State SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Ixodes scapularis; gynandromorphism; tick morphology; blacklegged tick; neoteny AB Gynandromorphism, the simultaneous occurrence of both male and female genotypic and morphological characteristics in a single individual of a normally sexually dimorphic species, is rare in ticks. The phenomenon is documented previously for free-living specimens representing several tick genera, particularly Amblyomma and Hyalomma, but only rarely in Ixodes. Here we describe the first two known gynandromorphs of the blacklegged tick, Ixodes scapularis Say, collected while flagging vegetation during routine tick surveillance in the Hudson Valley region of New York State. Uniquely, both specimens display some morphological features typical of nymphs, in addition to those of both males and females. C1 [Prusinski, M. A.] Bur Communicable Dis Control, New York State Dept Hlth, Vector Ecol Lab, Wadsworth Ctr,Biggs Labs, Albany, NY 12237 USA. [Meehan, L. J.] Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Sci Serv,Natl Vet Serv Lab, Ames, IA 50010 USA. [Meehan, L. J.] Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Technol Serv,Natl Vet Serv Lab, Ames, IA 50010 USA. [Meehan, L. J.] Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Anal Serv,Natl Vet Serv Lab, Ames, IA 50010 USA. RP Prusinski, MA (reprint author), Bur Communicable Dis Control, New York State Dept Hlth, Vector Ecol Lab, Wadsworth Ctr,Biggs Labs, C-456-C467B,Empire State Plaza, Albany, NY 12237 USA. EM melissa.prusinski@health.ny.gov FU New York State Department of Health; U. S. National Institutes of Health [AI097137] FX We are grateful to Alexey Khodjakov and Eric McVey for translations of Russian literature sources. We also thank Jeffery T. Alfred (NVSL) and Adam J. Bell (New York State Museum, Research and Collections) for contributing photographic images. This work was funded by the New York State Department of Health and U. S. National Institutes of Health grant AI097137. NR 17 TC 2 Z9 2 U1 1 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2015 VL 52 IS 2 BP 278 EP 282 DI 10.1093/jme/tjv009 PG 5 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CF2UE UT WOS:000352402300021 PM 26336313 ER PT J AU Bai, FL Tian, H Yu, QZ Ren, GP Li, DS AF Bai, F. L. Tian, H. Yu, Q. Z. Ren, G. P. Li, D. S. TI Expressing foreign genes by Newcastle disease virus for cancer therapy SO MOLECULAR BIOLOGY LA English DT Review DE rNDV-FG; cancer therapy; cancer immunotherapy; virotherapy ID MODIFIED TUMOR-CELLS; APOPTOSIS-INDUCING LIGAND; INNATE IMMUNE-RESPONSES; NATURAL-KILLER-CELLS; HEMAGGLUTININ-NEURAMINIDASE; ONCOLYTIC VIRUS; MALIGNANT-MELANOMA; INTERFERON-ALPHA; PROSTATE-CANCER; VACCINE VECTOR AB An interesting aspect of Newcastle disease virus (NDV) is the ability to selectively replicate in tumor cells. Recently, using reverse genetics technology to enhance the oncolytic properties and therapeutic potential of NDV for tumor therapy has become popular in immunocompetent carcinoma tumor models. Expressing foreign genes by recombinant NDV (rNDV-FG) has been shown to be more effective in cancer therapy in preclinical studies. This paper provides an overview of the current studies on the cytotoxic and anti-cancer effects of rNDV-FG via direct oncolysis and immune stimulation. Safety of rNDV-FG as a therapeutic agent for cancer immunotherapy and virotherapy is also discussed. C1 [Bai, F. L.; Ren, G. P.; Li, D. S.] Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China. [Bai, F. L.; Tian, H.] Lubin Environm Equipment Protect Co Ltd, Dept Res & Dev, Shanghai 200125, Peoples R China. [Yu, Q. Z.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Bai, FL (reprint author), Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China. EM desganli@163.com FU Medical Research Council; Technology and Innovation Fund of Qin Pengchun; Northeast Agriculture University FX The study was supported by a grant from the Medical Research Council, Technology and Innovation Fund of Qin Pengchun and a student fellowship from the Northeast Agriculture University. NR 65 TC 1 Z9 1 U1 0 U2 15 PU MAIK NAUKA/INTERPERIODICA/SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 USA SN 0026-8933 EI 1608-3245 J9 MOL BIOL+ JI Mol. Biol. PD MAR PY 2015 VL 49 IS 2 BP 171 EP 178 DI 10.1134/S0026893315020028 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CF9RC UT WOS:000352902500001 ER PT J AU Nelson, MI Viboud, C Vincent, AL Culhane, MR Detmer, SE Wentworth, DE Rambaut, A Suchard, MA Holmes, EC Lemey, P AF Nelson, Martha I. Viboud, Cecile Vincent, Amy L. Culhane, Marie R. Detmer, Susan E. Wentworth, David E. Rambaut, Andrew Suchard, Marc A. Holmes, Edward C. Lemey, Philippe TI Global migration of influenza A viruses in swine SO NATURE COMMUNICATIONS LA English DT Article ID PANDEMIC H1N1 INFLUENZA; NORTH-AMERICAN SWINE; PIG-POPULATION; UNITED-STATES; ORIGIN; REASSORTMENT; EVOLUTION; HUMANS; TRANSMISSION; SEQUENCE AB The complex and unresolved evolutionary origins of the 2009 H1N1 influenza pandemic exposed major gaps in our knowledge of the global spatial ecology and evolution of influenza A viruses in swine (swIAVs). Here we undertake an expansive phylogenetic analysis of swIAV sequence data and demonstrate that the global live swine trade strongly predicts the spatial dissemination of swIAVs, with Europe and North America acting as sources of viruses in Asian countries. In contrast, China has the world's largest swine population but is not a major exporter of live swine, and is not an important source of swIAVs in neighbouring Asian countries or globally. A meta-population simulation model incorporating trade data predicts that the global ecology of swIAVs is more complex than previously thought, and the United States and China's large swine populations are unlikely to be representative of swIAV diversity in their respective geographic regions, requiring independent surveillance efforts throughout Latin America and Asia. C1 [Nelson, Martha I.; Viboud, Cecile; Rambaut, Andrew] NIH, Div Int Epidemiol & Populat Studies, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Vincent, Amy L.] ARS, Virus & Prion Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Culhane, Marie R.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. [Detmer, Susan E.] Univ Saskatchewan, Western Coll Vet Med, Saskatoon, SK S7N 5A8, Canada. [Wentworth, David E.] J Craig Venter Inst, Rockville, MD 20850 USA. [Rambaut, Andrew] Univ Edinburgh, Inst Evolutionary Biol, Ashworth Labs, Edinburgh EH9 FLT, Midlothian, Scotland. [Rambaut, Andrew] Univ Edinburgh, Ctr Immunol Infect & Evolut, Ashworth Labs, Edinburgh EH9 FLT, Midlothian, Scotland. [Suchard, Marc A.] Univ Calif Los Angeles, Dept Biomath, Los Angeles, CA 90095 USA. [Suchard, Marc A.] Univ Calif Los Angeles, Dept Biostat, Los Angeles, CA 90095 USA. [Suchard, Marc A.] Univ Calif Los Angeles, Dept Human Genet, Los Angeles, CA 90095 USA. [Holmes, Edward C.] Univ Sydney, Charles Perkins Ctr, Sch Biol Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia. [Holmes, Edward C.] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. [Lemey, Philippe] Univ Leuven, KU Leuven, Dept Microbiol & Immunol, Rega Inst, Leuven, Belgium. RP Nelson, MI (reprint author), NIH, Div Int Epidemiol & Populat Studies, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM nelsonma@mail.nih.gov OI Rambaut, Andrew/0000-0003-4337-3707; Wentworth, David/0000-0002-5190-980X; Holmes, Edward/0000-0001-9596-3552 FU Saskatchewan Ministry of Agriculture [C70077]; Multinational Influenza Seasonal Mortality Study (MISMS); Office of Pandemics and Emerging Threats at the U.S. Department of Health and Human Services; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN272200900007C]; European Union Seventh Framework Programme (FP7) [278433-PREDEMICS]; ERC [260864]; NHMRC Australia Fellowship [AF30] FX We thank Drs Sagar Goyal and Devi Patnayak, along with Wendy Wiese, Becca Wheeldon and Lotus Smasal at UMVDL for their virology work, and Rebecca Halpin, Nadia Fedorova, Xudong Lin and Timothy B. Stockwell at the J. Craig Venter Institute for their assistance in viral sequencing. Aaron Schwartzbard was greatly helpful in producing figures using R. This manuscript was prepared while D.E.W. was employed at the J. Craig Venter Institute. Partial support for this work was provided by a grant from the Saskatchewan Ministry of Agriculture contract number C70077. This work was supported in part by the Multinational Influenza Seasonal Mortality Study (MISMS), an on-going international collaborative effort to understand influenza epidemiology and evolution, led by the Fogarty International Center, National Institutes of Health, with funding from Office of Pandemics and Emerging Threats at the U.S. Department of Health and Human Services (A.R., M.I.N., C.V.). This project has also been funded in part with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services under the (contract number HHSN272200900007C). P.L., M.A.S. and A. R. acknowledge the funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant Agreement no. 278433-PREDEMICS and ERC Grant agreement no. 260864. E.C.H. is supported by an NHMRC Australia Fellowship (AF30). NR 50 TC 16 Z9 17 U1 3 U2 26 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAR PY 2015 VL 6 AR 6696 DI 10.1038/ncomms7696 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CG1OY UT WOS:000353043900001 PM 25813399 ER PT J AU Hofmeyr, JH Carpenter, JE Bloem, S Slabbert, JP Hofmeyr, M Groenewald, SS AF Hofmeyr, J. H. Carpenter, J. E. Bloem, S. Slabbert, J. P. Hofmeyr, M. Groenewald, S. S. TI Development of the sterile insect technique to suppress false codling moth Thaumatotibia leucotreta (Lepidoptera: Tortricidae) in citrus fruit: Research to implementation (Part 1) SO AFRICAN ENTOMOLOGY LA English DT Article DE false codling moth; sterile insect technique; ionizing radiation; mass-rearing; commercialization ID MEYRICK LEPIDOPTERA AB False codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is indigenous to sub-Saharan Africa and infests a large number of commercial and wild fruit-bearing plants. The pest was unknown in the Western Cape Province of South Africa until the end of the 1960s, when it was first identified in fruit orchards in the Paarl region. By the late 1970s T. leucotreta was well established on citrus and other host plants in the Olifants River Valley, approximately 180 km north of Paarl. Since then, control measures for the pest included a small number of insecticides, mating disruption and orchard sanitation. However, a longer term solution was needed and research on the sterile insect technique was initiated in 2002. This initially involved three phases: a study of the radiation biology and F-1 sterility of T. leucotreta (phase 1), field cage experiments (phase 2) and a pilot project over 35 ha of T. leucotreta-susceptible citrus (phase 3). Favourable results in these studies led to the design and construction of T. leucotreta-specific rearing equipment and the building of a mass-rearing facility capable of producing up to 21 million insects per week (phase 4). Commercial sterile insect releases over 1500 ha of citrus orchards in the Citrusdal region commenced in 2007-08. This area was expanded to 3000 ha in 2008-09 and 4000 hectares in 2009-10 (phase 5). Over the three years the status of T. leucotreta as a pest threat was systematically reduced in the sterile insect release area compared to the non-release area. Feral male populations were reduced 3-, 8- and 10-fold, pre-harvest crop losses decreased by 50 %, 80% and 93 %, and post-harvest export fruit rejections in the SIT area dropped 13 %, 25 % and 38 %, respectively, compared to the non-SIT area. C1 [Hofmeyr, J. H.; Hofmeyr, M.] Citrus Res Int Pty Ltd, ZA-7340 Citrusdal, South Africa. [Carpenter, J. E.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Bloem, S.] USDA APHIS PPQ CPHST, Plant Epidemiol & Risk Anal Lab, Raleigh, NC USA. [Slabbert, J. P.] iThemba LABS, Dept Radiat Biophys, Somerset West, South Africa. [Groenewald, S. S.] Xsit Pty Ltd, ZA-7340 Citrusdal, South Africa. RP Hofmeyr, JH (reprint author), Citrus Res Int Pty Ltd, 108 Voortrekker St, ZA-7340 Citrusdal, South Africa. EM jhh@telkomsa.net FU citrus industry in South Africa FX We gratefully acknowledge B. Barnes (Agricultural Research Council, Infruitec, Stellenbosch, retired) for his co-operation in supplying laboratory and gamma radiation facilities. We thank S. Honiball (formerly with Ceder Biocontrol, Citrusdal), for the rearing of T. leucotreta used in our research. The support of the citrus industry in South Africa for the research project was invaluable. We also thank B. Barnes, as well as T. Grout (CRI, Nelspruit) and S. Moore (CRI, Port Elizabeth) for extensive reviews of the manuscript. NR 13 TC 7 Z9 7 U1 2 U2 7 PU ENTOMOLOGICAL SOC SOUTHERN AFRICA PI HATFIELD PA PO BOX 13162, HATFIELD 0028, SOUTH AFRICA SN 1021-3589 EI 1026-4914 J9 AFR ENTOMOL JI Afr. Entomol. PD MAR PY 2015 VL 23 IS 1 BP 180 EP 186 PG 7 WC Entomology SC Entomology GA CF3WC UT WOS:000352478600020 ER PT J AU Balkcom, KS Burmester, CH AF Balkcom, K. S. Burmester, C. H. TI Nitrogen Applications for Wheat Production across Tillage Systems in Alabama SO AGRONOMY JOURNAL LA English DT Article ID SOUTHEASTERN COASTAL-PLAIN; RED WINTER-WHEAT; UNITED-STATES; CONSERVATION TILLAGE; FERTILIZER NITROGEN; NO-TILL; COTTON PRODUCTION; SOIL; YIELD; ROTATION AB Alabama wheat (Triticum aestivum L.) farmers are changing management practices, which include using higher N fertilizer rates and planting wheat with no-tillage or other conservation tillage systems to maximize yields. Experiments were conducted to (i) determine the level of tillage necessary to optimize wheat yields across different regions of Alabama and (ii) determine if N requirements change across tillage systems and regions in Alabama at four locations resulting in 9 site-year comparisons. Each experiment consisted of a split-plot design with tillage as the main plot and 12 N fertilizer treatments as subplots, replicated four times to compare Zadoks' Growth Stage (GS)-30 tiller densities, tiller N concentrations, tiller biomass, GS-31 wheat biomass, biomass N concentration, wheat yields, and grain crude protein. Nitrogen treatments consisted of different rates across fall, GS-30, and GS-31 application times. Tillage systems had no effect on tiller density, tiller N concentration, or tiller biomass, but fall N increased tiller density 15% and tiller biomass 34% across Coastal Plain locations. Non-inversion tillage increased wheat yields 13% on Coastal Plain soils compared to conventional tillage. Fall N increased wheat yields 10%, and N applied at GS-30 improved yields 18% compared to delaying application until GS-31, indicating application of fall N and applying total N by GS-30 was imperative for successful wheat production on Coastal Plain soils. Neither tillage system nor N applications affected wheat production extensively across the Limestone Valley. Non-inversion tillage or no-tillage with current recommended N practices can be successfully used in Alabama wheat production. C1 [Balkcom, K. S.] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Burmester, C. H.] Auburn Univ, Tennessee Valley Res & Extens Ctr, Crop Soil & Environm Sci Dept, Belle Mina, AL 35615 USA. RP Balkcom, KS (reprint author), ARS, USDA, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. EM kip.balkcom@ars.usda.gov FU Alabama Wheat and Feed Grains Commission; International Plant Nutrition Institute FX The authors would like to acknowledge support provided by the Alabama Wheat and Feed Grains Commission, as well as, the International Plant Nutrition Institute. Technical assistance from personnel located at the Alabama Agricultural Experiment Stations in Belle Mina, Fairhope, Headland, and Shorter, in addition, to support provided by Jeffrey Walker and Karl Mannschreck of USDA-ARS were very much appreciated. NR 38 TC 2 Z9 2 U1 3 U2 17 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 425 EP 434 DI 10.2134/agronj14.0132 PG 10 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100002 ER PT J AU Tewolde, H Shankle, MW Way, TR Adeli, A Brooks, JP He, ZQ AF Tewolde, Haile Shankle, Mark W. Way, Thomas R. Adeli, Ardeshir Brooks, John P. He, Zhongqi TI Enhancing Management of Fall-Applied Poultry Litter with Cover Crop and Subsurface Band Placement in No-Till Cotton SO AGRONOMY JOURNAL LA English DT Article ID BROILER LITTER; PLANT-PARTS; UPLAND SOIL; NITROGEN; YIELD; NITRATE; FERTILIZATION; ROTATION; GROWTH; WATER AB Whether the yield reduction risk in cotton (Gossypium birsutum L.) fertilized with fall-applied poultry litter, relative to spring-applied litter, in regions with warm falls or winters can be minimized by applying the litter in subsurface bands along with a winter cover crop is unknown. A field study was conducted in Mississippi to determine whether litter applied in fall can be as effective as litter applied in spring for no-till cotton if applied in narrow subsurface bands (SSB) in the presence of a winter wheat (Triticum aestivum L.) cover crop (WCC). The study compared 5.6 Mg ha(-1) litter applied in the fall vs. spring, in SSB vs. surface broadcast (BC), with a WCC vs. no cover crop (NCC) in a 2 x 2 x 2 treatment structure. An unfertilized control and a treatment fertilized with conventional inorganic fertilizers were included. The results showed that planting wheat as a WCC, relative to NCC, increased cotton lint yield by as much as 18% in the driest season. Applying litter in fall leads to loss of litter-derived nutrients if applied by BC, but the losses are reduced if it is applied as SSB. Applying litter as SSB in conjunction with a WCC increased cotton lint yield relative to BC application with NCC by as much as 22%. The combined benefit of cover crop and subsurface banding suggests that poultry litter may be applied in fall in these soils without yield penalty, with potential to reduce the litter rate and minimize environmental contamination. C1 [Tewolde, Haile; Adeli, Ardeshir; Brooks, John P.] ARS, USDA, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [Shankle, Mark W.] Mississippi State Univ, Pontotoc Ridge Flatwoods Branch Expt Stn, Pontotoc, MS 38863 USA. [Way, Thomas R.] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [He, Zhongqi] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Tewolde, H (reprint author), ARS, USDA, Crop Sci Res Lab, Mississippi State, MS 39762 USA. EM haile.tewolde@ars.usda.gov NR 25 TC 2 Z9 2 U1 2 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 449 EP 458 DI 10.2134/agronj14.0266 PG 10 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100005 ER PT J AU Yost, MA Russelle, MP Coulter, JA Schmitt, MA Sheaffer, CC Randall, GW AF Yost, Matt A. Russelle, Michael P. Coulter, Jeffrey A. Schmitt, Michael A. Sheaffer, Craig C. Randall, Gyles W. TI Stand Age Affects Fertilizer Nitrogen Response in First-Year Corn following Alfalfa SO AGRONOMY JOURNAL LA English DT Article ID YIELD RESPONSE; CROPPING SYSTEM; SOIL; AVAILABILITY; RECOMMENDATIONS; TILLAGE; NITRATE; PLANTS AB Knowledge about how stand age affects N response in first-year corn (Zea mays L.) following alfalfa (Medicago sativa L.) is needed to improve N credit predictions. In a 3-yr study, we evaluated response to N in first-year corn following fall-terminated 1-to 3-yr-old alfalfa stands with stand densities of 33 to 579 plants m(-2) at Lamberton and Waseca, MN, each year. At both locations, alfalfa stand age had minor impacts on soil inorganic N content and aboveground corn dry matter (DM) yield, N concentration, and N uptake during first-year corn. On medium-textured soil at Lamberton, first-year corn following 3-yr-old alfalfa stands required no fertilizer N to economically optimize grain yield, but required 64 and 97 kg N ha(-1) following 2-and 1-yr-old stands, respectively, at the fertilizer cost (US$ kg(-1) N)/corn grain price ($ kg(-1) grain) ratio of 5.6. At the same price ratio on fine-textured soil at Waseca, first-year corn required 94 kg N ha-1 following both 2-and 3-yr-old stands and 118 kg N ha-1 following 1-yr-old stands. Therefore, current alfalfa N credit guidelines from land-grant universities that are based on alfalfa stand density at termination were not accurate for alfalfa stands <= 3 yr old that frequently had high densities. These results also confirrm that alfalfa stand age affects the frequency and level of fertilizer N response in first-year corn, and support the role of soil texture in altering the effect of stand age. C1 [Yost, Matt A.; Coulter, Jeffrey A.; Sheaffer, Craig C.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Russelle, Michael P.] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. [Schmitt, Michael A.; Randall, Gyles W.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Yost, MA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall,1991 Upper Buford Cr, St Paul, MN 55108 USA. EM yostma@missouri.edu NR 40 TC 1 Z9 1 U1 3 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 486 EP 494 DI 10.2134/agronj14.0493 PG 9 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100009 ER PT J AU Zhao, DL Glaz, B Irey, MS Hu, CJ AF Zhao, Duli Glaz, Barry Irey, Michael S. Hu, Chen-Jian TI Sugarcane Genotype Variation in Leaf Photosynthesis Properties and Yield as Affected by Mill Mud Application SO AGRONOMY JOURNAL LA English DT Article ID CHLOROPHYLL METER; GROWTH-RESPONSES; WATER-TABLE; SAND SOIL; REFLECTANCE; STRESS; DEFICIENCY; WHEAT; REGISTRATION; FLUORESCENCE AB Variability in yield among sugarcane (a complex hybrid of Saccharum spp.) genotypes grown with and without mill mud application on sand soils in Florida has been documented, but little is known about what causes yield differences and if there are any relationships between yield components and physiological parameters. This study determined responses of leaf relative chlorophyll level (SPAD readings), photochemical efficiency of photosystem II in dark-adapted leaves (Fv/Fm), leaf stomatal conductance (g(s)), net photosynthetic rate (Pn), transpiration rate (E), difference between leaf and air temperatures (Tl-Ta), and spectral reflectance in one Erianthus [Erianthus arundinaceus (Rez.) Jeswiet] and seven sugarcane genotypes and their relationships to yield components across genotypes. Reflectance, Fv/Fm, SPAD, gs, Pn, E, and Tl-Ta were measured on leaves at the top visible dewlap during the first- and second-ratoon crops on a Margate sand soil (siliceous, hyperthermic Mollic Psammaquent) with or without mill mud added before planting. Mill mud application reduced leaf reflectance at wavelengths of 560 and 710 nm and significantly increased leaf SPAD, gs, Pn, and E in the first-ratoon crop only. Genotypic differences were detected in most physiological traits and yield components. Most physiological traits correlated with cane and sucrose yields when no mill mud was added. Genotypic variation in physiological and yield responses to mill mud application and genotype x mill mud interactions in yields indicated that augmenting yield selection of genotypes with leaf physiological traits will improve sugarcane breeding programs for sand soils. C1 [Zhao, Duli; Glaz, Barry] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Irey, Michael S.; Hu, Chen-Jian] US Sugar Corp, Clewiston, FL 33440 USA. RP Zhao, DL (reprint author), ARS, USDA, Sugarcane Field Stn, US Highway 441 N, Canal Point, FL 33438 USA. EM duli.zhao@ars.usda.gov NR 47 TC 1 Z9 1 U1 1 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 506 EP 514 DI 10.2134/agronj14.0401 PG 9 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100011 ER PT J AU Lenssen, AW Sainju, UM Jabro, JD Allen, BL Evans, RG AF Lenssen, A. W. Sainju, U. M. Jabro, J. D. Allen, B. L. Evans, R. G. TI Management and Tillage Influence Barley Forage Productivity and Water Use in Dryland Cropping Systems SO AGRONOMY JOURNAL LA English DT Article ID NORTHERN GREAT-PLAINS; KOCHIA KOCHIA-SCOPARIA; WILD OAT; SEEDING RATE; WEED MANAGEMENT; HORDEUM-VULGARE; NUTRITIVE-VALUE; WESTERN CANADA; ZERO-TILLAGE; LOW-RAINFALL AB Annual cereal forages are resilient in water use (WU), water use efficiency (WUE), and weed control compared with grain crops in dryland systems. The combined influence of tillage and management systems on annual cereal forage productivity and WU is not well documented. We conducted a field study for the effects of tillage (no-till and tilled) and management (ecological and conventional) systems on WU and performance of forage barley (Hordeum vulgare L.) and weed biomass in two crop rotations (wheat [Triticum aestivum L.]-forage barley-pea [Pisum sativum L.] and wheat-forage barley-corn [Zea mays L.] -pea) from 2004 to 2010 in eastern Montana. Conventional management included recommended seeding rates, broadcast N fertilization, and short stubble height of wheat. Ecological management included 33% greater seeding rates, banded N fertilization at planting, and taller wheat stubble. Forage barley in ecological management had 28 more plants m(-2), 2 cm greater height, 65 more tillers m(-2), 606 kg ha(-1) greater crop biomass, 3.5 kg ha(-1) mm(-1) greater WUE, and 47% reduction in weed biomass at harvest than in conventional management. Pre-plant and post-harvest soil water contents were similar among tillage and management systems, but barley WU was 13 mm greater in 4-yr than 3-yr rotation. Tillage had little effect on barley performance and WU. Dryland forage barley with higher seeding rate and banded N fertilization in more diversified rotation produced more yield and used water more efficiently than that with conventional seeding rate, broadcast N fertilization, and less diversified rotation in the semiarid northern Great Plains. C1 [Lenssen, A. W.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Lenssen, A. W.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT USA. [Sainju, U. M.; Jabro, J. D.; Allen, B. L.; Evans, R. G.] ARS, USDA, ASRU, Sidney, MT 59270 USA. RP Lenssen, AW (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM alenssen@iastate.edu NR 40 TC 0 Z9 0 U1 3 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 551 EP 557 DI 10.2134/agronj14.0421 PG 7 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100016 ER PT J AU Landry, EJ Coyne, CJ Hu, JG AF Landry, Erik J. Coyne, Clarice J. Hu, Jinguo TI Agronomic Performance of Spring-Sown Faba Bean in Southeastern Washington SO AGRONOMY JOURNAL LA English DT Article ID VICIA-FABA; DROUGHT TOLERANCE; YIELD; ADAPTATION; VARIETIES AB Faba bean (Vicia faba L.) is the world's fourth most important cool-season pulse crop. Currently commercial faba bean cultivars are unavailable in the United States specifically selected for grain production; therefore, a spring-sown variety trial with 11 populations was conducted for two seasons at two contrasting locations in southeastern Washington. Early seeding was expected to achieve optimal grain yield. In 2012, the warmer Central Ferry, WA, location was sown a month earlier than at Pullman, WA (April vs. May), resulting in earlier lowering, higher grain yield, and an earlier maturity across populations. A drier March-April in 2013 allowed similar sowing dates across locations, synchronizing phenological development and improving grain yields at Pullman. However, at Central Ferry, yield was reduced due to high temperatures (> 35 degrees C) and subsequent lower and pod shedding. The National Plant Germplasm System (NPGS) accession W6 12023 and the Extra Precoce Violetto x Hiverna/2 F-3:(5) breeding population were the highest yielding populations and earliest to lower and mature in 2012 and 2013, respectively. Across 4 site-years, W6 12023 proved to have superior general adaptability with a high mean plant yield (22.6 g plant(-1)). Yield data compared favorably to other spring sown pulses currently grown in the region; therefore, a multi-environment trial with early maturing spring-type cultivars is warranted. C1 [Landry, Erik J.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Coyne, Clarice J.; Hu, Jinguo] Washington State Univ, ARS, USDA, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. RP Hu, JG (reprint author), Washington State Univ, ARS, USDA, Western Reg Plant Intro Stn, Johnson Hall 59,POB 6402, Pullman, WA 99164 USA. EM jinguo.hu@ars.usda.gov FU USDA-ARS CRIS Project [5348-21000-026-00D]; NIFA MultiState Project [W006]; ARCS Foundation FX Funding from USDA-ARS CRIS Project 5348-21000-026-00D, NIFA MultiState Project W006, and a scholarship from the ARCS Foundation to E. Landry. We gratefully acknowledge the assistance with planting and plot maintenance by Wayne Olson, Sean Vail, and Kurt Tetrick. We would also like to thank Dr. Wolfgang Link for providing seed. NR 29 TC 4 Z9 4 U1 1 U2 3 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 574 EP 578 DI 10.2134/agronj14.0284 PG 5 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100019 ER PT J AU Tomasek, BJ Williams, MM Davis, AS AF Tomasek, Bradley J. Williams, Martin M., II Davis, Adam S. TI Optimization of Agricultural Field Workability Predictions for Improved Risk Management SO AGRONOMY JOURNAL LA English DT Article ID SOIL WORKABILITY; ORGANIC-MATTER; UNITED-STATES; TRENDS; CLIMATE; PRECIPITATION; VARIABILITY; SIMULATION; TEXTURE; MODELS AB Improved prediction of the days available for field work, or field working days (FWDs), is an important consideration for adapting farming systems to increased weather variability. We developed modeling approaches to estimate robust soil moisture thresholds for FWDs. We used simulated soil moisture to train the model on the same type of data that would be used for FWD forecasting (prediction). These new models were tested against previously suggested thresholds for field workability. Model 1 used historical field work and weather records from three crop research centers in a logistic regression model. A soil moisture threshold of 1.10 times the plastic limit (1.10PL) was identified. Model 2 identified statewide soil moisture and temperature thresholds by optimizing the root mean square error of the predicted number of weekly statewide FWDs across a 52-yr data set. The resulting thresholds of either 0.88PL or 0.73FC (field capacity) and an average temperature requirement of at least 6 degrees C yielded statistically smaller absolute errors for the state average FWDs and in eight of the nine crop reporting districts. The Model 2 thresholds also eliminated systematic overprediction present in previous thresholds. These results demonstrate that immutable theoretical thresholds for FWDs based on field-measured soil moisture can be suboptimal for prediction at a larger spatial scale due to consistent bias. C1 [Tomasek, Bradley J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Williams, Martin M., II; Davis, Adam S.] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Tomasek, BJ (reprint author), Univ Illinois, Dept Crop Sci, S-306 Turner Hall, Urbana, IL 61801 USA. EM tomasek3@illinois.edu FU USDA-ARS [3611-22000-018] FX We gratefully acknowledge the work of Kurt Spokas in updating the Soil and Moisture Model. Support for this work was provided by the USDA-ARS (Project 3611-22000-018). NR 36 TC 1 Z9 1 U1 1 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 627 EP 633 DI 10.2134/agronj14.0393 PG 7 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100025 ER PT J AU Glaz, B Wiersma, J Hernandez, JA Martin, NF Yeater, KM AF Glaz, Barry Wiersma, Jochum Hernandez, Jose A. Martin, Nicolas F. Yeater, Kathleen M. TI Introduction to the Statistical Concepts Symposium Section: Selected Review Topics to Improve Our Understanding and Use of Statistics SO AGRONOMY JOURNAL LA English DT Article C1 [Glaz, Barry; Wiersma, Jochum] Univ Minnesota, Northwest Res & Outreach Ctr, Crookston, MN 56716 USA. [Hernandez, Jose A.] Univ Minnesota, St Paul, MN 55108 USA. [Martin, Nicolas F.] Syngenta Biotechnol, Stanton, MN USA. [Yeater, Kathleen M.] ARS, USDA, PA NRRC, Ft Collins, CO 80526 USA. EM barry.glaz@gmail.com NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 691 EP 691 DI 10.2134/agronj14.0888 PG 1 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100032 ER PT J AU Casler, MD AF Casler, Michael D. TI Fundamentals of Experimental Design: Guidelines for Designing Successful Experiments SO AGRONOMY JOURNAL LA English DT Article ID RESPONSE-SURFACE DESIGNS; MODIFIED AUGMENTED DESIGN; COMPLETE-BLOCK-DESIGNS; FIELD EXPERIMENTS; CONTROL PLOTS; RELATIVE EFFICIENCY; GENETIC PROGRESS; SELECTION; TRIALS; POPULATIONS AB We often think of experimental designs as analogous to recipes in a cookbook. We look for something that we like, something that satisfies our needs, and frequently return to those that have become our long-standing favorites. We can easily become complacent, favoring the tried-and-true designs (or recipes) over those that contain unknown or untried ingredients or those that are too complex for our tastes and skills. Instead, I prefer to think of experimental designs as a creative series of decisions that are meant to solve one or more problems. These problems may be real or imagined-we may have direct evidence of a past or current problem or we may simply want insurance against future potential problems. The most significant manifestation of a "problem" or a "failed" design is unsatisfactory P values that prevent us from developing inferences about treatment differences. Four basic tenets or pillars of experimental design- replication, randomization, blocking, and size of experimental units-can be used creatively, intelligently, and consciously to solve both real and perceived problems in comparative experiments. Because research is expensive, both in terms of grant funds and the emotional costs invested in grant competition and administration, biological experiments should be designed under the mantra "failure is not an option." Guidelines and advice provided in this review are designed to reduce the probability of failure for researchers who are willing to question, evaluate, and possibly modify their decision-making processes. C1 ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Casler, MD (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr W, Madison, WI 53706 USA. EM michaelcasler@ars.usda.gov NR 56 TC 7 Z9 7 U1 8 U2 18 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 692 EP 705 DI 10.2134/agronj2013.0114 PG 14 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100033 ER PT J AU Campbell, KG Thompson, YM Guy, SO McIntosh, M Glaz, B AF Campbell, Kimberly Garland Thompson, Yvonne M. Guy, Stephen O. McIntosh, Marla Glaz, Barry TI "Is, or is not, the two great ends of Fate": Errors in Agronomic Research SO AGRONOMY JOURNAL LA English DT Article ID STATISTICAL SIGNIFICANCE; SIGNIFICANCE TESTS; PRACTICAL GUIDE; TEST CRITERIA; EFFECT SIZE; POWER; EQUIVALENCE; INFERENCE; PURPOSES; IMPACTS AB Agronomic research results include Type 1 (alpha) and Type 2 (beta) errors. Results are often reported using a alpha <= 0.05 while beta is ignored. Our objective was to discuss whether a false positive was more serious than a false negative in agronomic research. For comparison, current statistical methods used in Agronomy Journal were tabulated. Most papers used null hypothesis tests with a alpha <= 0.05, reporting results based on the LSD among all treatment pairs. Current practices do not account for the relative costs of false positive vs. false negative errors. A case study from the Washington State Wheat Extension trials was analyzed using mixed models with specific contrasts. While the overall effect for cultivar was significant, the beta error rate for the contrast was 40% and additional replications were needed to increase the power of this contrast. A second case study analyzed trials evaluating wheat (Triticum aestivum L.) resistance to Fusarium crown rot. Optimal alpha and beta error rates were estimated for two to eight replications with the Type1/Type2 error cost ratio set at 1:1 and 1:5. An average error rate (alpha and beta) <= 0.05 could be achieved with four replications when a reduction in the beta error was critical and a errors could be corrected in future experiments. Effective experimental design requires estimation of the acceptable magnitude and cost ratio of false positive and false negative errors and critical effect sizes. To be truly informative, reports of results should include this information plus observed effect sizes and variances. C1 [Campbell, Kimberly Garland] Washington State Univ, ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Campbell, Kimberly Garland; Thompson, Yvonne M.; Guy, Stephen O.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [McIntosh, Marla] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Glaz, Barry] ARS, USDA, Sugarcane Prod Res Unit, Canal Point, FL 33438 USA. RP Campbell, KG (reprint author), Washington State Univ, ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. EM kim.garland-campbell@ars.usda.gov FU USDA-ARS [5348-21220-003-00D]; Washington Grains Commission [3019-3548, 3019-6345, 4150-1216] FX We acknowledge the following funding sources: USDA-ARS (in-house) Project 5348-21220-003-00D and Washington Grains Commission Projects 3019-3548, 3019-6345, and 4150-1216. Thanks also to two anonymous reviewers and their detailed comments and to the senior author's graduate advisor, the highly successful tobacco breeder, Dr. E.A. Wernsman, who inspired the topic by stating, "I based my career on insignificant differences." NR 42 TC 0 Z9 0 U1 3 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 718 EP 729 DI 10.2134/agronj14.0167 PG 12 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100035 ER PT J AU Vargas, M Glaz, B Alvarado, G Pietragalla, J Morgounov, A Zelenskiy, Y Crossa, J AF Vargas, Mateo Glaz, Barry Alvarado, Gregorio Pietragalla, Julian Morgounov, Alexey Zelenskiy, Yuriy Crossa, Jose TI Analysis and Interpretation of Interactions in Agricultural Research SO AGRONOMY JOURNAL LA English DT Article ID MULTIPLE COMPARISON PROCEDURES; SITES REGRESSION; MONTE-CARLO; ATTRIBUTES; TRIALS; MODEL AB When reporting on well-conducted research, a characteristic of a complete and proper manuscript is one that includes analyses and interpretations of all interactions. Our purpose is to show how to analyze and interpret interactions in agronomy and breeding research by means of three data sets comprising random and fixed effects. Experiment 1 tested wheat (Triticum aestivum L.) at two N and four P fertilizer rates in two soil types. For this data set, we used a fixed-effect linear model with the highest order (three-way) interaction considered first and then worked down through the lower order interactions and main effects to illustrate the importance of interactions in data analysis. Experiment 2 evaluated maize (Zea mays L.) hybrids under four rates of N for 3 yr. For this data set, we used a linear mixed model and partitioned the four N rates into orthogonal polynomials. Experiment 3 evaluated genotypes in six environments where the objective was to show how to study genotype x environment interactions. Researchers must analyze all interactions, determine if they are due to changes in rank (crossover) or only to changes in scale, and then judge whether reporting on significant main effects or interactions would best explain the biological responses in their experiments. In an experiment with more than one factor, complete and correct analysis of interactions is essential for reporting and interpreting the research properly. C1 [Vargas, Mateo] Univ Autonoma Chapingo, Chapingo, Mexico. [Alvarado, Gregorio; Crossa, Jose] Int Maize & Wheat Improvement Ctr CIMMYT, Biometr & Stat Unit, Mexico City 06600, DF, Mexico. [Glaz, Barry] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Pietragalla, Julian] Int Maize & Wheat Improvement Ctr CIMMYT, Global Wheat Breeding Program, Mexico City 06600, DF, Mexico. [Morgounov, Alexey] CIMMYT, TR-06511 Ankara, Turkey. [Zelenskiy, Yuriy] CIMMYT, Astana 010000, Kazakhstan. RP Crossa, J (reprint author), Int Maize & Wheat Improvement Ctr CIMMYT, Biometr & Stat Unit, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. EM j.crossa@cgiar.org OI Crossa, Jose/0000-0001-9429-5855 NR 17 TC 1 Z9 1 U1 1 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 748 EP 762 DI 10.2134/agronj13.0405 PG 15 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100038 ER PT J AU Yeater, KM Duke, SE Riedell, WE AF Yeater, Kathleen M. Duke, Sara E. Riedell, Walter E. TI Multivariate Analysis: Greater Insights into Complex Systems SO AGRONOMY JOURNAL LA English DT Article ID CANONICAL DISCRIMINANT-ANALYSIS; WEED SPECIES ABUNDANCE; IDENTIFYING ASSOCIATIONS; GENETIC-VARIATION; SITE PROPERTIES; YIELD; SOIL; WHEAT AB Many agronomic researchers measure and collect multiple response variables in an effort to understand the more complex nature of the system being studied. Multivariate (MV) statistical methods encompass the simultaneous analysis of all random variables measured on each experimental or sampling unit. Many agronomic research systems studied are, by their very nature, MV; however, most analyses reported are univariate (analysis of one response at a time). The objective of this review is to outline a statistical foundation of applications of MV methods and techniques for the agronomic sciences. By utilizing two agronomic data sets, both typical in dimension and structure, we discuss three classes of MV techniques based on the research question and characteristics of the data: (i) hypothesis driven, such as MV analysis of variance; (ii) dimension reduction, such as principal components analysis; and (iii) classification and discrimination, which includes canonical discriminant analysis. Several advantages and disadvantages of the MV tools are explained. This review will provide researchers with a beginning framework of MV generalizations of univariate techniques, and methods that are unique to MV dimension analysis. It is important for researchers to capture the concept of variability within a MV data set to better understand the complex system. C1 [Yeater, Kathleen M.] ARS, USDA, Southern Plains Area, College Stn, TX 77840 USA. [Duke, Sara E.] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Riedell, Walter E.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Yeater, KM (reprint author), ARS, USDA, Southern Plains Area, 1001 Holleman Dr E, College Stn, TX 77840 USA. EM kathleen.yeater@ars.usda.gov NR 39 TC 5 Z9 6 U1 4 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2015 VL 107 IS 2 BP 799 EP 810 DI 10.2134/agronj14.0017 PG 12 WC Agronomy SC Agriculture GA CE9JV UT WOS:000352161100042 ER PT J AU Jiang, HQ Radtke, PJ Weiskittel, AR Coulston, JW Guertin, PJ AF Jiang, Huiquan Radtke, Philip J. Weiskittel, Aaron R. Coulston, John W. Guertin, Patrick J. TI Climate- and soil-based models of site productivity in eastern US tree species SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE bootstrap; climate change; climate envelope models; random forest; regression trees; site index ID WESTERN UNITED-STATES; FOREST INVENTORY; INDEX; PINE; PREDICTION; VARIABLES; REGRESSION; SPRUCE; PLANTATIONS; STANDS AB As concerns rise over potential effects of greenhouse gas related climate change on terrestrial ecosystems, forest managers require growth and yield modeling capabilities responsive to changing climate conditions. Our goal was to develop prediction models of site index for eastern US forest tree species with climate and soil properties as predictors for use in predicting potential responses of forest productivity to climate change. Species-specific site index data from the USDA Forest Service Forest Inventory and Analysis (FIA) program were linked to contemporary climate data and soil properties mapped in the USDA Soil Survey Geographic (SSURGO) database. Random forest regression tree based ensemble prediction models of site index were constructed based on 37 climate-related and 15 soil attributes. In addition to a species-specific site index, aggregate models were developed for species grouped into two broad categories: conifer (softwood) and hardwood (broadleaved) species groups. Species-specific models based on climate and soil predictors explained the most variation in site index of any models tested (R-2 = 62.5%, RMSE = 3.2 m). Comparable results were found when grouping species into conifer and hardwood groups (R-2 = 63.9%, RMSE = 4.6 m for conifers; R-2 = 35.9%, RMSE = 4.2 m for hardwoods). Model predictions based on multiple global circulation models (GCMs) and Intergovernmental Panel on Climate Change (IPCC) development scenarios were tested for statistical significance using bootstrap resampling methods. Results showed significant increases over the 21st century in mean site index for conifers between +0.5 and +2.4 m. Over the same time period, mean hardwood site index showed decreases of as much as -1.7 m for the scenarios tested. The results demonstrate the utility of using climate and soils data in predicting site index across a large geographic region, and the potential of climate change to alter forest productivity in the eastern US. Additional investigation is needed to interpret spatial patterns and ecological relationships related to predictions from this type of model. C1 [Jiang, Huiquan; Radtke, Philip J.] Virginia Tech, Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Weiskittel, Aaron R.] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Coulston, John W.] ARS, USDA, Knoxville, TN 37919 USA. [Guertin, Patrick J.] US Army Engn Res & Dev Ctr, Construct Engn Res Lab, Champaign, IL 61826 USA. RP Radtke, PJ (reprint author), Virginia Tech, Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. EM pradtke@vt.edu OI Radtke, Philip/0000-0002-8921-8406 FU U.S. Department of the Army; Southern Appalachian Mountains (SAM) Cooperative Ecosystem Studies Unit (CESU) FX This research was supported with funding from the U.S. Department of the Army in cooperation with the Southern Appalachian Mountains (SAM) Cooperative Ecosystem Studies Unit (CESU). NR 74 TC 2 Z9 3 U1 4 U2 23 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAR PY 2015 VL 45 IS 3 BP 325 EP 342 DI 10.1139/cjfr-2014-0054 PG 18 WC Forestry SC Forestry GA CF0CX UT WOS:000352211400011 ER PT J AU Eichenberger, S Miguez, F Edwards, J Knapp, A AF Eichenberger, Steve Miguez, Fernando Edwards, Jode Knapp, Allen TI Changes in Kernel Filling with Selection for Grain Yield in a Maize Population SO CROP SCIENCE LA English DT Article ID ZEA-MAYS L.; RECIPROCAL RECURRENT SELECTION; HIGH PLANT-DENSITY; SOURCE-SINK RATIO; WATER RELATIONS; HALF-SIB; ADAPTATION; MORPHOLOGY; GERMPLASM; DURATION AB Genetic improvement in kernel filling characteristics has been an important contributor to hybrid maize (Zea mays L.) yield increases. However, changes in kernel filling characteristics have not been evaluated within the context of recurrent selection programs. The objective of this study was to determine if rate and duration of kernel filling and their interaction with plant density changed with long-term recurrent selection for agronomic performance in the Iowa Stiff Stalk Synthetic (BSSS) population. Populations selected for improved grain yield and agronomic performance were compared with the unselected base population, BSSS, at average final plant densities of 4.7 and 6.6 seeds m(-2) at two locations near Ames, IA in 2009 and 2010. Treatments were replicated three times per location in a split plot design. Selected populations had a longer period of kernel filling but did not have a higher rate of kernel filling. Selected populations also had reduced final kernel weight at higher plant density, whereas unselected populations did not differ in final kernel weight. Reduction in final kernel weight at high plant density in selected populations suggests that increases in grain yield at high plant density in selected populations resulted from components of grain yield other than final kernel weight. C1 [Eichenberger, Steve] Monsanto Co, Cedar Falls, IA 50613 USA. [Miguez, Fernando; Knapp, Allen] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Edwards, Jode] ARS, USDA, CICGRU, Ames, IA 50011 USA. RP Edwards, J (reprint author), ARS, USDA, CICGRU, Agron Hall,100 Osborn Dr, Ames, IA 50011 USA. EM jode.edwards@ars.usda.gov NR 19 TC 2 Z9 2 U1 2 U2 18 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 521 EP 526 DI 10.2135/cropsci2014.07.0462 PG 6 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000006 ER PT J AU Edwards, JW Orellana, M AF Edwards, Jode W. Orellana, Massiel TI Increasing Selection Response by Bayesian Modeling of Heterogeneous Environmental Variances SO CROP SCIENCE LA English DT Article ID MIXED LINEAR-MODELS; PERFORMANCE; TRIALS; CORN AB Heterogeneity of nongenetic variances among genotypes reduces selection response because high-variance genotypes are more likely to have means in the tail of the distribution and thus more likely to be selected than low-variance genotypes. The bias towards high-variance genotypes is difficult to correct in selection programs because nongenetic variances must be estimated for each genotype with small sample size per genotype. We have tested a Bayesian approach to modeling heterogeneous variances among genotypes to test whether the Bayesian approach provides sufficient correction for variance heterogeneity to improve selection response. Data were simulated using a broad range of parameters and analyzed with a heterogeneous-variance model and homogeneous-variance model using Bayesian estimation. Selection was performed at intensities of 3.125 and 12.5% on the basis of Bayesian estimates of genotypic values with simulated data. Parameter values for heterogeneity of variances were chosen from previously published analyses of cultivar trials in maize ( Zea mays L.) and oat ( Avena sativa L.) in Iowa. With four to eight replications ( i.e., four to eight environments with one replicate per environment) and intense selection, we estimated that modeling of heterogeneous variances with Bayesian estimation could increase selection response by up to 15%. C1 [Edwards, Jode W.] Iowa State Univ, Dept Agron, USDA ARS CICGRU, Ames, IA 50011 USA. [Orellana, Massiel] Monsanto Co, Creve Coeur, MO 63141 USA. RP Edwards, JW (reprint author), Iowa State Univ, Dept Agron, USDA ARS CICGRU, Ames, IA 50011 USA. EM jode.edwards@ars.usda.gov NR 17 TC 0 Z9 0 U1 1 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 556 EP 563 DI 10.2135/cropsci2014.08.0582 PG 8 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000009 ER PT J AU Yerka, MK Watson, A Toy, JJ Erickson, G Pedersen, JF Mitchell, R AF Yerka, Melinda K. Watson, Andrea Toy, J. J. Erickson, Galen Pedersen, Jeffrey F. Mitchell, Rob TI Yield and Forage Value of a Dual-Purpose bmr-12 Sorghum Hybrid SO CROP SCIENCE LA English DT Article ID BROWN MIDRIB SORGHUM; LACTATING DAIRY-COWS; BICOLOR L. MOENCH; CORN-SILAGE; PEARL-MILLET; ETHANOL YIELD; GRAIN-SORGHUM; QUALITY; SUDANGRASS; MAIZE AB Grain sorghum [Sorghum bicolor (L.) Moench] is an important crop for rainfed production systems with 2.7 million ha grown in the United States in 2013. The brown-midrib (bmr) mutations, especially bmr-12, have resulted in low stover lignin and high fiber digestibility without reducing grain yield in some sorghum lines. However, the effect of the bmr trait on beef cattle (Bos taurus) performance when grazing crop residue is unknown. Our objectives were to validate previous small-plot results reporting no grain yield difference between near-isogenic bmr-12 (BMR) and wild-type control (CON) A Wheatland x R Tx430 sorghum hybrids in a field-scale experiment and to determine if BMR stover enhances beef production in a grazing experiment. Four replicated paddocks (2.3 ha) were planted in 2006 and 2008 near Mead, NE. Crossbred yearling steers (240 +/- 17 kg hd(-1)) grazed (2.6 steers ha(-1)) paddocks following grain harvest for 72 d in 2006 and 61 d in 2008. Forage was sampled 4, 30, and 60 d after grazing began. Grain yield of BMR was 6% less (P = 0.01) than CON with no difference in stover neutral detergent fiber (NDF) content, but BMR stover had higher in vitro NDF digestibility (IVNDFD) (31%; P < 0.0001), steer average daily gain (ADG; 0.18 kg hd(-1) d(-1); P = 0.001), and body weight (BW) gain (29 kg ha(-1); P = 0.002), resulting in an estimated increase in net return of $133.84 ha(-1) due to BMR. Results suggest that the A Wheatland x R Tx430 bmr-12 hybrid is an effective dual-purpose sorghum crop for both grain and beef production. C1 [Yerka, Melinda K.; Toy, J. J.; Pedersen, Jeffrey F.; Mitchell, Rob] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Watson, Andrea; Erickson, Galen] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. RP Yerka, MK (reprint author), Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. EM melinda.yerka@ars.usda.gov NR 36 TC 1 Z9 1 U1 6 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 681 EP 687 DI 10.2135/cropsci2014.06.0437 PG 7 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000022 ER PT J AU Xu, XY Bai, GH Carver, BF Zhan, KH Huang, YH Mornhinweg, D AF Xu, Xiangyang Bai, Guihua Carver, Brett F. Zhan, Kehui Huang, Yinghua Mornhinweg, Dolores TI Evaluation and Reselection of Wheat Resistance to Russian Wheat Aphid Biotype 2 SO CROP SCIENCE LA English DT Article ID RYE TRANSLOCATION LINES; DIURAPHIS-NOXIA; WINTER-WHEAT; REGISTRATION; GENES; INHERITANCE; GERMPLASM; MARKERS; IDENTIFICATION; POPULATIONS AB Russian wheat aphid (RWA, Diuraphis noxia, Mordvilko) biotype 2 (RWA2) is virulent to most known RWA resistance genes and severely threatens wheat (Triticum aestivum L.) production in the hard winter wheat area of the U.S. western Great Plains. We determined RWA2 reactions of 386 cultivars from China, 227 advanced breeding lines and recently released cultivars from the United States, 505 landraces from countries where RWA is endemic, and 31 genetic stocks developed in the United States, Australia, Canada, and Russia. The majority of wheat accessions from China and the United States were highly susceptible to RWA2. Only nine landrace accessions produced a homogeneous resistant reaction. In addition, highly resistant plants were identified in 28 heterogeneous landraces. Thus, reselection was conducted to purify some potential resistance sources, and the single-plant progenies of 220 selected plants were evaluated. Homogeneous resistant or highly resistant lines were identified from seven previously heterogeneous landraces. Reselection line PI 626759-20-32 offered a high level of resistance similar to lines carrying Dn7, the rye (Secale cereale L.)-derived resistance gene associated with undesirable bread-making quality. PI 626759-20-32 has the potential to supplement or replace Dn7 as a new RWA2 resistance source in wheat breeding. C1 [Xu, Xiangyang; Huang, Yinghua; Mornhinweg, Dolores] USDA ARS, Wheat Peanut & Other Field Crop Res Unit, Stillwater, OK 74075 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Carver, Brett F.] Oklahoma State Univ, Plant & Soil Sci Dept, Stillwater, OK 74075 USA. [Zhan, Kehui] Henan Agr Univ, Dept Agron, Zhengzou 450002, Peoples R China. RP Xu, XY (reprint author), USDA ARS, Wheat Peanut & Other Field Crop Res Unit, Stillwater, OK 74075 USA. EM xiangyang.xu@ars.usda.gov NR 40 TC 1 Z9 1 U1 4 U2 13 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 695 EP 701 DI 10.2135/cropsci2014.07.0473 PG 7 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000024 ER PT J AU Belamkar, V Wenger, A Kalberer, SR Bhattacharya, VG Blackmon, WJ Cannon, SB AF Belamkar, Vikas Wenger, Alex Kalberer, Scott R. Bhattacharya, V. Gautam Blackmon, William J. Cannon, Steven B. TI Evaluation of Phenotypic Variation in a Collection of Apios americana: An Edible Tuberous Legume SO CROP SCIENCE LA English DT Article ID POPULATIONS; ISOFLAVONE; POTATO; MEDIK; SEED AB Apios (Apios americana Medik.), sometimes called "potato bean," is a nitrogen-fixing legume, native to eastern North America, that produces protein-rich tubers at nodes along belowground stolons. Apios was used as a staple food source by Native Americans throughout eastern North America and holds promise as a crop. An Apios breeding program conducted during 1985 to 1994 involved the collection of similar to 210 wild accessions, followed by hybridization and selection, with assessments of > 2200 lines. Of these, 53 genotypes were retained for further evaluation. The study reports the phenotypic variation in this collection, at three locations and under three growing conditions (field, pots, and grow-bags). We found significant (P < 0.05) variation among the genotypes for 18 of the 20 measured traits under field conditions, and for seven of 20 traits in pots and grow-bags. Internode length, plant vigor, and stem diameter at 2 and 5 mo had strong correlations (r > 0.56, P < 0.01) with belowground yield plant(-1). Four phenotypically distinct clusters of genotypes were evident in the Apios collection. Several genotypes produced high yields in all locations-up to 1515 g of belowground tuber yield plant-1. The superior germplasm identified in this project may be suitable as cultivars, and will aid in further development of Apios lines as a crop. C1 [Belamkar, Vikas] Iowa State Univ, Interdept Genet, Ames, IA 50011 USA. [Belamkar, Vikas; Cannon, Steven B.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Kalberer, Scott R.; Cannon, Steven B.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Cannon, SB (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM steven.cannon@ars.usda.gov NR 28 TC 2 Z9 2 U1 3 U2 14 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 712 EP 726 DI 10.2135/cropsci2014.04.0281 PG 15 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000026 ER PT J AU Hinze, LL Horn, PJ Kothari, N Dever, JK Frelichowski, J Chapman, KD Percy, RG AF Hinze, Lori L. Horn, Patrick J. Kothari, Neha Dever, Jane K. Frelichowski, James Chapman, Kent D. Percy, Richard G. TI Nondestructive Measurements of Cottonseed Nutritional Trait Diversity in the US National Cotton Germplasm Collection SO CROP SCIENCE LA English DT Article ID SEED OIL CONTENT; FOLIAR APPLICATION; PROTEIN; FERTILIZATION; VARIABILITY; QUALITY; YIELDS; ZINC; ENVIRONMENT; PHOSPHORUS AB Recent studies have suggested that cottonseed (Gossypium spp.) has the potential to contribute to the effort against world hunger, particularly by providing a high-quality protein source. This report analyzed the diversity in protein content and other seed quality factors in the U.S. National Cotton Germplasm Collection. Nine genomes (one tetraploid and eight diploid) and 33 species (five tetraploid and 28 diploid) were surveyed in this examination of 2256 accessions. A novel nondestructive nuclear magnetic resonance technique was applied to measure oil and protein content, seed indices were calculated, and these data were associated with molecular marker information. Oil content ranged from 8 to 27%, protein values ranged from 10 to 36%, and seed index was lowest at 1 g per 100 seeds and extended up to 18 g per 100 seeds. Most of the range in values for these traits resided within G. hirsutum L. and G. barbadense L.; thus implying that variability for cottonseed quality can be introduced with relative ease in current breeding programs. The diploid genomes generally had extremely low values for oil, protein, and seed index. Molecular marker information indicated that chromosome 21 was likely associated with oil content. Understanding how these seed quality factors vary independently and in relation to each other will allow us to better select parents for breeding programs, and identifying associations with molecular markers may help us enhance progress through marker-assisted selection approaches. C1 [Hinze, Lori L.; Frelichowski, James; Percy, Richard G.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Horn, Patrick J.; Chapman, Kent D.] Univ N Texas, Dept Biol Sci, Ctr Plant Lipid Res, Denton, TX 76203 USA. [Kothari, Neha; Dever, Jane K.] Texas AgriLife Res, Dept Soil & Crop Sci, Lubbock, TX 79403 USA. RP Hinze, LL (reprint author), USDA ARS, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. EM Lori.Hinze@ars.usda.gov RI Kothari, Neha/K-8184-2016; OI Kothari, Neha/0000-0001-6184-1784; Hinze, Lori L/0000-0003-3356-1245 FU CRIS of the U.S. Department of Agriculture [6202-21000-031-00D]; Cotton Incorporated [08-395] FX This research project was supported by funding from CRIS No. 6202-21000-031-00D of the U.S. Department of Agriculture. Seed analyses by time-domain 1H-NMR was supported by agreement 08-395 from Cotton Incorporated to KDC. The authors gratefully acknowledge support staff from the USDA-ARS cotton genetics and breeding project in College Station, TX, and from the University of North Texas Center for Plant Lipid Research for their valuable assistance gathering the accession samples and measuring the seed quality traits. NR 40 TC 9 Z9 9 U1 0 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 770 EP 782 DI 10.2135/cropsci2014.04.0318 PG 13 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000031 ER PT J AU Burrell, AM Sharma, A Patil, NY Collins, SD Anderson, WF Rooney, WL Klein, PE AF Burrell, A. Millie Sharma, Arun Patil, Nikhil Y. Collins, S. Delroy Anderson, William F. Rooney, William L. Klein, Patricia E. TI Sequencing of an Anthracnose-Resistant Sorghum Genotype and Mapping of a Major QTL Reveal Strong Candidate Genes for Anthracnose Resistance SO CROP SCIENCE LA English DT Article ID BICOLOR L. MOENCH; COLLETOTRICHUM-SUBLINEOLUM; HIGHER-PLANTS; LEAF-BLIGHT; IDENTIFICATION; EVOLUTION; MARKERS; GENOME; IMPROVEMENT; FEEDSTOCK AB Anthracnose, caused by the fungal pathogen Colletotrichum sublineolum Henn. ex. Sacc. and Trotter 1913, is an economically damaging disease of sorghum [Sorghum bicolor (L.) Moench] in hot and humid production regions of the world. Control of anthracnose is almost exclusively through the use of genetic resistance. To further elucidate genetic resistance to anthracnose, a recombinant inbred line population derived from the cross of BTx623 (susceptible) and SC748-5 (resistant) was created. A linkage map was constructed using 117 F-5 individuals that were genotyped using Digital Genotyping, a genotyping-by-sequencing method developed specifically for C4 grasses, on an Illumina GAIIx. The linkage map consists of 619 single nucleotide polymorphism markers and three microsatellites with a total map length of 1269.9 cM. The population was phenotyped for anthracnose in four different environments. Using both composite interval mapping and inclusive composite interval mapping (ICIM), one major quantitative trait locus (QTL) on chromosome 5 was consistently identified as the source of anthracnose resistance in all environments. Sequencing genomic DNA from SC748-5 and comparison to BTx623 genomic sequence revealed numerous amino acid changes in annotated disease-resistance genes located in the area under the anthracnose QTL. This suggests that the genetic architecture for anthracnose resistance in SC748-5 is not under the control of one gene but, more likely, a link-age block containing several resistance genes. C1 [Burrell, A. Millie; Sharma, Arun; Patil, Nikhil Y.; Klein, Patricia E.] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Collins, S. Delroy; Rooney, William L.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Anderson, William F.] USDA ARS, Tifton, GA 31793 USA. RP Klein, PE (reprint author), Texas A&M Univ, Dept Hort Sci, 2133 TAMUS, College Stn, TX 77843 USA. EM pklein@tamu.edu FU Ceres, Inc. FX The authors wish to thank Ms. Natalie Patterson (Texas Agri-Life Research) for expert technical assistance. Additionally, we thank Dr. Louis Prom (USDA-ARS) for providing the colonized sorghum seeds containing mixed inoculum of multiple Colletotrichum sublineolum strains. This research was supported by Ceres, Inc. NR 63 TC 2 Z9 2 U1 3 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2015 VL 55 IS 2 BP 790 EP 799 DI 10.2135/cropsci2014.06.0430 PG 10 WC Agronomy SC Agriculture GA CF0HE UT WOS:000352223000033 ER PT J AU Cunniffe, NJ Koskella, B Metcalf, CJE Parnell, S Gottwald, TR Gilligan, CA AF Cunniffe, Nik J. Koskella, Britt Metcalf, C. Jessica E. Parnell, Stephen Gottwald, Tim R. Gilligan, Christopher A. TI Thirteen challenges in modelling plant diseases SO EPIDEMICS LA English DT Article DE Plant disease model; Plant epidemiology; Compartmental models; Stochastic models; Landscape-scale models ID THEORETICAL ASSESSMENT; CROP DISEASE; PATHOGEN; SPREAD; EPIDEMICS; EVOLUTION; VIRUS; SCALE; DISPERSAL; NETWORKS AB The underlying structure of epidemiological models, and the questions that models can be used to address, do not necessarily depend on the host organism in question. This means that certain preoccupations of plant disease modellers are similar to those of modellers of diseases in human, livestock and wild animal populations. However, a number of aspects of plant epidemiology are very distinctive, and this leads to specific challenges in modelling plant diseases, which in turn sets a certain agenda for modellers. Here we outline a selection of 13 challenges, specific to plant disease epidemiology, that we feel are important targets for future work. (C) 2014 The Authors. Published by Elsevier B.V. C1 [Cunniffe, Nik J.; Gilligan, Christopher A.] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England. [Koskella, Britt] Univ Exeter, BioSci, Tremough TR10 9EZ, England. [Metcalf, C. Jessica E.] Univ Oxford, Dept Zool, Oxford OX1 3PS, England. [Parnell, Stephen] Rothamsted Res, Computat & Syst Biol, Harpenden AL5 2JQ, Herts, England. [Gottwald, Tim R.] USDA ARS, Ft Pierce, FL 34945 USA. RP Cunniffe, NJ (reprint author), Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England. EM njc1001@cam.ac.uk RI parnell, stephen/I-7682-2015; Koskella, Britt/A-7398-2008; OI parnell, stephen/0000-0002-2625-4557; Koskella, Britt/0000-0003-1760-8496; Cunniffe, Nik/0000-0002-3533-8672 NR 37 TC 10 Z9 10 U1 3 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1755-4365 J9 EPIDEMICS-NETH JI Epidemics PD MAR PY 2015 VL 10 BP 6 EP 10 DI 10.1016/j.epidem.2014.06.002 PG 5 WC Infectious Diseases SC Infectious Diseases GA CF0IO UT WOS:000352226900003 PM 25843374 ER PT J AU Mojica, L Meyer, A Berhow, MA de Mejia, EG AF Mojica, Luis Meyer, Allison Berhow, Mark A. de Mejia, Elmira Gonzalez TI Bean cultivars (Phaseolus vulgaris L.) have similar high antioxidant capacity, in vitro inhibition of alpha-amylase and alpha-glucosidase while diverse phenolic composition and concentration SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE Common bean; Anthocyanins; Phenolics; Antioxidant capacity; Color; alpha-Glucosidase; alpha-Amylase ID COMMON BEANS; RICH EXTRACTS; NITRIC-OXIDE; ANTHOCYANINS; INFLAMMATION; PROFILES; PINTO AB Common beans are a good source of essential nutrients such as protein, fiber, vitamins, and minerals; they also contain phenolic compounds and other phytochemicals. Phenolic compounds exhibit high antioxidant capacity that promotes health benefits by reducing oxidative stress. The objective was to compare the composition and quantity of anthocyanins and other non-colored phenolic compounds in fifteen improved bean cultivars from Mexico and Brazil and their relation to antioxidant capacity and enzymes related to type-2 diabetes. Samples were analyzed for total phenolic compounds (TP), flavonoids, antioxidant capacity (AC), tannins and total anthocyanins. Type and quantity were evaluated by HPLC-ESI-MS. Delphinidin glucoside (0.9-129.0 mg/100 g dry coat), petunidin glucoside (0.7-115.0 mg/100 g dry coat) and malvidin glucoside (0.14-52.0 mg/100 g dry coat). Anthocyanidins were positively correlated when quantified by HPLC and colorimetric analysis (R = 0.99). Cultivar Negro-Otomi presented the highest concentration of anthocyanins (250 mg/100 g dry coat). Seventeen non-colored phenolic compounds were identified among cultivars; catechin, myricetin 3-O-arabinoside, epicatechin, vanillic acid, syringic acid and o-coumaric acid, presented the highest concentrations among identified phenolic compounds. The AC of all fifteen bean cultivars did not show significant differences (p > 0.05) ranging from 185.2 (FM-67) to 233.9 (FM-199) mmol TE/g coat. Compounds in the coat extracts of pinto and black cultivars were the most efficient to inhibit alpha-amylase and alpha-glucosidase. Studied cultivars differed in composition and concentration of phenolics including anthocyanins; however, there was no effect on AC as measured by oxygen radical absorbance capacity. Black beans contained delphinidin and ferulic acid, compounds commonly used as ingredients in functional foods due to their associated health benefits. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Mojica, Luis; Meyer, Allison; de Mejia, Elmira Gonzalez] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Berhow, Mark A.] ARS, USDA, Peoria, IL 61604 USA. RP de Mejia, EG (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. EM edemejia@illinois.edu FU Consejo Nacional de Ciencia y Tecnologia CONACyT-Mexico [215989]; Hatch Grant University of Illinois [ILLU-698-384] FX The authors are thankful to Ing. Jose Angel Cid Rios, Dr. Jorge Alberto Acosta Gallegos and Dr. Hercia Stampini Duarte Marino for providing the bean samples, and the Lemann Center at the University of Illinois for funding. Author Luis Mojica was supported by a scholarship from the Consejo Nacional de Ciencia y Tecnologia CONACyT-Mexico (215989). Research was supported by funding from a Hatch Grant (ILLU-698-384), University of Illinois. NR 41 TC 17 Z9 17 U1 11 U2 60 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD MAR PY 2015 VL 69 BP 38 EP 48 DI 10.1016/j.foodres.2014.12.007 PG 11 WC Food Science & Technology SC Food Science & Technology GA CE9OT UT WOS:000352174000005 ER PT J AU Juneja, VK Cadavez, V Gonzales-Barron, U Mukhopadhyay, S AF Juneja, Vijay K. Cadavez, Vasco Gonzales-Barron, Ursula Mukhopadhyay, Sudarsan TI Modelling the effect of pH, sodium chloride and sodium pyrophosphate on the thermal resistance of Escherichia coli O157:H7 in ground beef SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE E. coli; Minced beef; Weibull; Polynomial; Predictive model; Thermal lethality ID LISTERIA-MONOCYTOGENES; HEAT-RESISTANCE; HEMORRHAGIC COLITIS; INACTIVATION MODEL; SURVIVAL CURVES; WEIBULL MODEL; UNITED-STATES; O157-H7; TEMPERATURE; DESCRIBE AB The objective of this study was to assess the combined effects of temperature, pH, sodium chloride (NaCl), and sodium pyrophosphate (SPP) on the heat resistance of Escherichia coli O157:H7 in minced beef meat. A fractional factorial design consisted of four internal temperatures (55.0, 57.5, 60.0 and 62.5 degrees C), five concentrations of NaCl (0.0, 1.5, 3.0, 4.5 and 6.0 wt/wt%) and SPP (0.0, 0.1, 0.15, 0.2 and 03 wt/wt%), and five levels of pH (4.0, 5.0, 6.0, 7.0 and 8.0). The 38 variable combinations were replicated twice to provide a total of 76 survivor curves, which were modelled by a modified three-parameter Weibull function as primary model. The polynomial secondary models, developed to estimate the time to achieve a 3-log and a 5-log reduction, enabled the estimation of critical pH, NaCl and SPP concentrations, which are values at which the thermo-tolerance of E. coli O157:H7 reaches it maximum. The addition up to a certain critical concentration of NaCl (similar to 2.7-4.7%) or SPP (similar to 0.16%) acts independently to increase the heat resistance of E. coli O157:H7. Beyond such critical concentrations, the thermo-resistance of E. coli O157:H7 will progressively diminish. A similar pattern was found for pH with a critical value between 6.0 and 6.7, depending upon temperature and NaCl concentration. A mixed-effects omnibus regression model further revealed that the acidity of the matrix and NaCl concentration had a greater impact on the inactivation kinetics of E coli O157:H7 in minced beef than SPP, and both are responsible for the concavity/convexity of the curves. When pH, SPP or NaCl concentration is far above or below from its critical value, the temperatures needed to reduce E. coli O157:H7 up to a certain log level are much lower than those required when any other environmental condition is at its critical value. Meat processors can use the model to design lethality treatments in order to achieve specific log reductions of E. coli O157:H7 in ready-to-eat beef products. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Juneja, Vijay K.; Mukhopadhyay, Sudarsan] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Cadavez, Vasco; Gonzales-Barron, Ursula] Polytech Inst Broganzo, Sch Agr, CIMO Mt Res Ctr, Oporto, Portugal. RP Gonzales-Barron, U (reprint author), Polytech Inst Broganzo, Sch Agr, CIMO Mt Res Ctr, Oporto, Portugal. EM ubarron@ipb.pt RI Cadavez, Vasco/A-3958-2010; OI Cadavez, Vasco/0000-0002-3077-7414; Gonzales-Barron, Ursula/0000-0002-8462-9775 FU Portuguese Foundation for Science and Technology (FCT) [IF/00570] FX The authors thank Ms. Angie Osoria for her technical assistance. Dr. Gonzales-Barron wishes to acknowledge the financial support provided by the Portuguese Foundation for Science and Technology (FCT) through the award of a five-year Investigator Fellowship (IF) in the mode of Development Grants (IF/00570). NR 39 TC 2 Z9 2 U1 6 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD MAR PY 2015 VL 69 BP 289 EP 304 DI 10.1016/j.foodres.2014.11.050 PG 16 WC Food Science & Technology SC Food Science & Technology GA CE9OT UT WOS:000352174000035 ER PT J AU Brandeis, C Hodges, DG AF Brandeis, Consuelo Hodges, Donald G. TI Forest Sector and Primary Forest Products Industry Contributions to the Economies of the Southern States: 2011 Update SO JOURNAL OF FORESTRY LA English DT Article DE southern forest products industry; economic contribution; economic contribution; timber harvest; North American Industry Classification System (NAICS) AB The analysis in this article provides an update on the southern forest sector economic activity after the downturn experienced in 2008-2009. The analysis was conducted using Impact Analysis for Planning (IMPLAN) software and data sets for 2009 and 2011 and results from the USDA Forest Service Timber Products Output latest survey of primary wood processing mills. Although improving economic conditions are reflected by increased mill roundwood consumption during 2011, the forest industry's economic contribution improved slightly but not across all states. At the regional scale, the sector displayed a downward trend in employment, value added, and number of active primary mills. C1 [Brandeis, Consuelo] USDA, Forest Serv Forest Inventory & Anal, Knoxville, TN 37919 USA. [Hodges, Donald G.] Univ Tennessee, Knoxville, TN 37996 USA. RP Brandeis, C (reprint author), USDA, Forest Serv Forest Inventory & Anal, Knoxville, TN 37919 USA. EM cbrandeis@fs.fed.us; dhodges2@utk.edu OI Hodges, Donald/0000-0001-6751-0927 NR 18 TC 3 Z9 3 U1 1 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2015 VL 113 IS 2 BP 205 EP 209 DI 10.5849/jof.14-054 PG 5 WC Forestry SC Forestry GA CE8VY UT WOS:000352123400006 ER PT J AU Pomeroy, M Bragg, DC AF Pomeroy, Michael Bragg, Don C. TI Ozark Badger Lumber Company's Innovative Approach to Selective Management SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Bragg, Don C.] US Forest Serv, USDA, Monticello, AR USA. EM mikepomeroy@midwesthardwood.com; braggd@safnet.org NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2015 VL 113 IS 2 BP 257 EP 257 DI 10.5849/jof.14-115 PG 1 WC Forestry SC Forestry GA CE8VY UT WOS:000352123400012 ER PT J AU Kenefic, LS Kern, CC AF Kenefic, Laura S. Kern, Christel C. TI Early Northern Hardwood Silvicultural Research at the Dukes Experimental Forest, Michigan SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Kenefic, Laura S.] US Forest Serv, USDA, No Res Stn, Orono, ME 04469 USA. [Kern, Christel C.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN USA. RP Kenefic, LS (reprint author), US Forest Serv, USDA, No Res Stn, Orono, ME 04469 USA. EM lkenefic@fs.fed.us; cckern@fs.fed.us NR 12 TC 0 Z9 0 U1 1 U2 1 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2015 VL 113 IS 2 BP 258 EP 261 DI 10.5849/jof.14-154 PG 4 WC Forestry SC Forestry GA CE8VY UT WOS:000352123400013 ER PT J AU Elias, PE Heath, LS AF Elias, Patricia E. Heath, Linda S. TI REDD plus and Ecosystem Services: Relevance to the Forestry Profession SO JOURNAL OF FORESTRY LA English DT Editorial Material ID DEFORESTATION C1 [Elias, Patricia E.] Elias Consulting LLC, St Albans, VT 05478 USA. [Heath, Linda S.] US Forest Serv, USDA, Durham, NH USA. RP Elias, PE (reprint author), Elias Consulting LLC, St Albans, VT 05478 USA. EM pipa.elias@gmail.com; Lheath@fs.fed.us NR 18 TC 0 Z9 0 U1 1 U2 3 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2015 VL 113 IS 2 BP 268 EP 270 DI 10.5849/jof.14-107 PG 3 WC Forestry SC Forestry GA CE8VY UT WOS:000352123400015 ER PT J AU Jurney, DH Hoagland, S AF Jurney, David H. Hoagland, Serra TI Bridging the Gaps That Divide SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Jurney, David H.] US Forest Serv, USDA, Russellville, AR 72801 USA. [Hoagland, Serra] US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, Russellville, AR USA. RP Jurney, DH (reprint author), US Forest Serv, USDA, Russellville, AR 72801 USA. EM djurney@fs.fed.us NR 4 TC 0 Z9 0 U1 1 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2015 VL 113 IS 2 BP 271 EP 272 DI 10.5849/jof.14-124 PG 2 WC Forestry SC Forestry GA CE8VY UT WOS:000352123400016 ER PT J AU Reddy, UK Abburi, L Abburi, VL Saminathan, T Cantrell, R Vajja, VG Reddy, R Tomason, YR Levi, A Wehner, TC Nimmakayala, P AF Reddy, Umesh K. Abburi, Lavanya Abburi, Venkata Lakshmi Saminathan, Thangasamy Cantrell, Robert Vajja, Venkata Gopinath Reddy, Rishi Tomason, Yan R. Levi, Amnon Wehner, Todd C. Nimmakayala, Padma TI A Genome-Wide Scan of Selective Sweeps and Association Mapping of Fruit Traits Using Microsatellite Markers in Watermelon SO JOURNAL OF HEREDITY LA English DT Article DE domestication; heterozygosity; linkage disequilibrium; population structure; recombination rate ID SIMPLE SEQUENCE REPEATS; SINGLE NUCLEOTIDE POLYMORPHISMS; LANATUS THUNB. MATSUM.; INTEGRATED GENETIC-MAP; CITRULLUS-LANATUS; POPULATION-STRUCTURE; DIVERSITY ANALYSIS; MAIZE; NAKAI; DOMESTICATION AB Our genetic diversity study uses microsatellites of known map position to estimate genome level population structure and linkage disequilibrium, and to identify genomic regions that have undergone selection during watermelon domestication and improvement. Thirty regions that showed evidence of selective sweep were scanned for the presence of candidate genes using the watermelon genome browser (www.icugi.org). We localized selective sweeps in intergenic regions, close to the promoters, and within the exons and introns of various genes. This study provided an evidence of convergent evolution for the presence of diverse ecotypes with special reference to American and European ecotypes. Our search for location of linked markers in the whole-genome draft sequence revealed that BVWS00358, a GA repeat microsatellite, is the GAGA type transcription factor located in the 5' untranslated regions of a structure and insertion element that expresses a Cys(2)His(2) Zinc finger motif, with presumed biological processes related to chitin response and transcriptional regulation. In addition, BVWS01708, an ATT repeat microsatellite, located in the promoter of a DTW domain-containing protein (Cla002761); and 2 other simple sequence repeats that association mapping link to fruit length and rind thickness. C1 [Reddy, Umesh K.; Abburi, Lavanya; Saminathan, Thangasamy; Cantrell, Robert; Vajja, Venkata Gopinath; Reddy, Rishi; Tomason, Yan R.; Nimmakayala, Padma] West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. [Levi, Amnon] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Wehner, Todd C.] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. RP Reddy, UK (reprint author), West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. EM ureddy@wvstateu.edu FU USDA-NIFA [2013-38821-21453]; NSF-EPSCOR [1003907]; Gus R. Douglass Institute; NIH [P20RR016477] FX USDA-NIFA (2013-38821-21453), NSF-EPSCOR#1003907, Gus R. Douglass Institute and NIH grant number P20RR016477. NR 46 TC 5 Z9 5 U1 2 U2 33 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 EI 1465-7333 J9 J HERED JI J. Hered. PD MAR-APR PY 2015 VL 106 IS 2 BP 166 EP 176 DI 10.1093/jhered/esu077 PG 11 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA CF1HV UT WOS:000352296600003 PM 25425675 ER PT J AU Plischuk, S Sanscrainte, ND Becnel, JJ Estep, AS Lange, CE AF Plischuk, Santiago Sanscrainte, Neil D. Becnel, James J. Estep, Alden S. Lange, Carlos E. TI Tubulinosema pampeana sp n. (Microsporidia, Tubulinosematidae), a pathogen of the South American bumble bee Bombus atratus SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Argentina; Bee health; Cyst-like bodies; Nosema; Pampas region; Pollinator ID NOSEMA-BOMBI; SEQUENCE ALIGNMENT; APIS-MELLIFERA; HONEY-BEE; PARASITE; HYMENOPTERA; APIDAE; CERANAE; RATISBONENSIS; PERFORMANCE AB An undescribed microsporidium was detected and isolated from the South American bumble bee Bombus atratus collected in the Pampas region of Argentina. Infection intensity in workers averaged 8.2 x 10(7) spores/bee. The main site of infection was adipose tissue where hypertrophy of adipocytes resulted in cyst-like body formation. Mature spores were ovoid and monomorphic. They measured 4.00 mu m x 2.37 mu m (fresh) or 3.98 mu m x 1.88 mu m (fixed). All stages were diplokariotic and developed in direct contact with host cytoplasm. Isofilar polar filament was arranged in 16 coils in one or, posteriorly, two layers. Coiling angle was variable, between perpendicular and almost parallel to major spore axis. Late meronts and sporogonial stages were surrounded by vesicles of approximately 60 nm in diameter. Based on both new and already designed primers, a 1827 bp (SSUrRNA, ITS, LSUrRNA) sequence was obtained. Data analyses suggest that this microsporidium is a new species of the genus Tubulinosema. The name Tubulinosema pampeana sp. n. is proposed. (C) 2015 Elsevier Inc. All rights reserved. C1 [Plischuk, Santiago; Lange, Carlos E.] UNLP, CCT La Plata CONICET, Ctr Estudios Parasitol & Vectores CEPAVE, La Plata, Buenos Aires, Argentina. [Sanscrainte, Neil D.; Becnel, James J.; Estep, Alden S.] ARS, CMAVE, USDA, Gainesville, FL USA. [Estep, Alden S.] Navy Entomol Ctr Excellence, Naval Air Stn, Jacksonville, FL USA. [Lange, Carlos E.] CIC, Buenos Aires, DF, Argentina. RP Plischuk, S (reprint author), Ctr Estudios Parasitol & Vectores CEPAVE, Blvd 120 E-60 & 64, RA-1900 La Plata, Buenos Aires, Argentina. FU USDA - ARS; CICPBA; CONICET [4754/12]; FONCyT [PICT 2012-0851, PICT 2012-0199] FX Authors are grateful to Susan Bjornson, Cory Stanley-Stahr, Mariano Higes, Lee lien Solter and two anonymous reviewers. SP also acknowledges IDEA WILD. This study was supported by USDA - ARS, CICPBA, CONICET (Fin. Res. 4754/12), and FONCyT (PICT 2012-0851 and PICT 2012-0199). NR 45 TC 2 Z9 3 U1 2 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAR PY 2015 VL 126 BP 31 EP 42 DI 10.1016/j.jip.2015.01.006 PG 12 WC Zoology SC Zoology GA CF6NL UT WOS:000352673300005 PM 25637516 ER PT J AU Casey, W Jacobs, A Maull, E Matheson, J Clarke, C Lowit, A AF Casey, Warren Jacobs, Abigail Maull, Elizabeth Matheson, Joanna Clarke, Carol Lowit, Anna TI A New Path Forward: The Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and National Toxicology Program's Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) SO JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE LA English DT Article AB In 2000, the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) was congressionally established, with representatives from Federal regulatory and research agencies that require, use, generate, or disseminate toxicologic and safety testing information. For over 15 y, ICCVAM and the National Toxicology Program's Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) have worked together to promote the development, validation, and regulatory acceptance of test methods that replace, reduce, or refine the use of animals in regulatory testing. In 2013, both NICEATM and ICCVAM underwent major changes to their operating paradigms, to increase the speed and efficiency of regulatory approval and industry adoption of 3Rs testing methods within the United States and internationally. Accordingly, increased emphasis has been placed on international activities, primarily through interaction with the Organization for Economic Cooperation and Development and participation in the International Cooperation on Alternative Test Methods. In addition, ICCVAM has committed to increasing public awareness of and transparency about federal agencies' 3R activities and to fostering interactions with stakeholders. Finally, although it continues to support ICCVAM, NICEATM's work now includes validation support for Tox21, a collaboration aimed at identifying in vitro methods and computational approaches for testing chemicals to better understand and predict hazards to humans and the environment. The combination of more efficient operating paradigms, increased international collaboration, improved communication and interaction with stakeholders, and active participation in Tox21 likely will substantially increase the number of 3Rs methods developed and used in the United States and internationally. C1 [Casey, Warren; Maull, Elizabeth] NIEHS, NIH, DNTP, NICEATM, Res Triangle Pk, NC 27709 USA. [Jacobs, Abigail] FDA, CDER, Silver Spring, MD USA. [Matheson, Joanna; Lowit, Anna] US Consumer Prod Safety Commiss, Bethesda, MD USA. [Clarke, Carol] USDA, Riverdale, MD USA. US EPA, Washington, DC 20460 USA. RP Casey, W (reprint author), NIEHS, NIH, DNTP, NICEATM, POB 12233, Res Triangle Pk, NC 27709 USA. EM warren.casey@nih.gov NR 3 TC 4 Z9 4 U1 0 U2 5 PU AMER ASSOC LABORATORY ANIMAL SCIENCE PI MEMPHIS PA 9190 CRESTWYN HILLS DR, MEMPHIS, TN 38125 USA SN 1559-6109 J9 J AM ASSOC LAB ANIM JI J. Amer. Assoc. Lab. Anim. Sci. PD MAR PY 2015 VL 54 IS 2 BP 170 EP 173 PG 4 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA CE8UJ UT WOS:000352119300008 PM 25836963 ER PT J AU Landolt, PJ Zack, RS Roberts, D AF Landolt, Peter J. Zack, Richard S. Roberts, Diana TI Seasonal response of Noctua pronuba Linnaeus, 1758 (Lepidoptera: Noctuidae) to traps in Washington State SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE yellow underwing; cutworm; attractant; blacklight; flight period; acetic acid; 3-methyl-1-butanol ID ATTRACTANTS; CUTWORM; LURES; BAITS AB Noctua pronuba Linnaeus, 1758 (Lepidoptera: Noctuidae), the yellow underwing moth, was recently introduced into western North America. Blacklight traps at multiple sites in eastern Washington State indicate two broad periods of moth flight. A spring flight was evident in May and June, and a late season flight was evident from late August into October. Noctua pronuba moths were captured also in traps baited with acetic acid plus 3-methyl-1-butanol, but not in traps baited with acetic acid or 3-methyl-1-butanol alone. This chemical combination is thought to be a feeding attractant for many noctuid moths and may be useful for monitoring N. pronuba. The seasonal pattern of moths captured in traps baited with this chemical lure was similar to the pattern seen with blacklight traps. C1 [Landolt, Peter J.] ARS, USDA, Wapato, WA 98951 USA. [Zack, Richard S.] Washington State Univ, Dept Entomol, James Entomol Collect, Pullman, WA 99164 USA. [Roberts, Diana] Washington State Univ, Extens Serv, Spokane, WA 99202 USA. RP Landolt, PJ (reprint author), ARS, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov; zack@wsu.edu; robertsd@wsu.edu FU Washington Tree Fruit Research Commission; Washington State Potato Commission; Washington Grain Commission FX Technical assistance was provided by Jewel Brumley, Gracie Galindo, Robin Garcia, and Daryl Green. We thank R. Halvorson and Hal Johnson for access to their farms, Jim Evans of the Nature Conservancy for access to the Swauk Creek Ranch, and Elwood Crozier and the Obert family for access to their apple orchards. This work was supported in part by funding from the Washington Tree Fruit Research Commission, Washington State Potato Commission, and the Washington Grain Commission. NR 30 TC 0 Z9 0 U1 2 U2 4 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD MAR PY 2015 VL 91 IS 1 BP 20 EP 28 PG 9 WC Entomology SC Entomology GA CF3ZE UT WOS:000352486900002 ER PT J AU Yee, WL Lawrence, TW Hood, GR Feder, JL AF Yee, Wee L. Lawrence, Tom W. Hood, Glen R. Feder, Jeffrey L. TI New records of Rhagoletis Loew, 1862 (Diptera: Tephritidae) and their host plants in western Montana, USA SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Western Montana; Rhagoletis berberis; Rhagoletis basiola; Rhagoletis tabellaria; Rhagoletis pomonella ID CHERRY FRUIT-FLY; WASHINGTON-STATE; APPLE MAGGOT; SPECIES DIPTERA; INDIFFERENS DIPTERA; BRITISH-COLUMBIA; POMONELLA; PHENOLOGY; RACES; SHAPE AB Little information exists concerning the distribution of fruit flies in the genus Rhagoletis Loew, 1862 (Diptera: Tephritidae) in Montana, western U.S.A. Here, the presence of and host plant use by Rhagoletis spp. are documented in northwestern Montana. The western cherry fruit fly, Rhagoletis indifferens Curran, 1932, was abundant in the state and infested sweet cherry, Prunus avium (L.) L.; mahaleb cherry, P. mahaleb L.; tart cherry, P. cerasus L.; and bitter cherry, P. emarginata (Douglas ex Hook.) D. Dietr. (Rosaceae). Black cherry fruit fly, R. fausta (Osten-Sacken, 1877), was rare and detected only in bitter cherry. Rhagoletis berberis Curran, 1932, R. basiola (Osten-Sacken, 1873), R. tabellaria (Fitch, 1855), and apple maggot, R. pomonella (Walsh, 1867), were all recorded for the first time in Montana. Flies in Montana were mainly reared from previously reported species of host plants, but new host records also documented includeR. indifferens from black hawthorn, Crataegus douglasii Lindl. (Rosaceae), a new Montana record; R. berberis from sweet cherry; R. basiola from baldhip rose, Rosa gymnocarpa Nutt. (Rosaceae); and R. tabellaria from Hooker's fairy bells, Prosartes hookeri Torr. (Liliaceae). Rhagoletis pomonella, likely introduced into the western U.S., was reared from C. douglasii but not from the relatively few domesticated apple trees, Malus domestica Borkh., (Rosaceae) sampled. The findings extend the known geographic ranges of four Rhagoletis species and indicate that some flies in northwestern Montana have the ability to survive and develop in alternative and novel hosts, consistent with findings for populations in other areas of the western U.S. C1 [Yee, Wee L.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Hood, Glen R.; Feder, Jeffrey L.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. RP Yee, WL (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov FU NSF; USDA; USDA-FAS FX We thank Janine Jewett and Pete Chapman (USDA-ARS) for laboratory assistance, Ed Lisowski (Washington State Department of Agriculture, Yakima, WA) and Dong Cha (USDA-ARS) for reviewing early drafts of the manuscript, and the NSF and USDA and the USDA-FAS for supporting the work of J.L.F. and W.L.Y., respectively. NR 56 TC 3 Z9 3 U1 1 U2 13 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD MAR PY 2015 VL 91 IS 1 BP 39 EP 57 PG 19 WC Entomology SC Entomology GA CF3ZE UT WOS:000352486900004 ER PT J AU Smith-Pardo, AH Beucke, K AF Smith-Pardo, Allan H. Beucke, Kyle TI Flat Bugs (Hemiptera: Heteroptera: Aradidae) intercepted at United States Ports of Entry SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE quarantine; reportable; diversity; invasive species; identification AB The flat bugs (Hemiptera: Aradidae) are primarily mycophagous, and some species may pose a threat to cultivated mushrooms. In addition, at least one species feeds on living plant tissue. For this reason, they are potentially of economic significance. Their identification below family level is sometimes difficult, partly due to the scattered nature of the literature. We present a list of the aradids intercepted at United States Ports of Entry from 1992 to March 2013. In addition, we provide keys and diagnoses for the identification of aradid subfamilies and the intercepted, reportable genera of the Aradidae. C1 [Smith-Pardo, Allan H.; Beucke, Kyle] USDA APHIS PPQ, Plant Inspect Stn, San Francisco, CA 94080 USA. RP Smith-Pardo, AH (reprint author), USDA APHIS PPQ, Plant Inspect Stn, 389 Oyster Point Blvd,Suite 2, San Francisco, CA 94080 USA. EM allan.h.smith-pardo@aphis.usda.gov NR 29 TC 0 Z9 0 U1 0 U2 2 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD MAR PY 2015 VL 91 IS 1 BP 58 EP 81 PG 24 WC Entomology SC Entomology GA CF3ZE UT WOS:000352486900005 ER PT J AU Hardigan, MA Bamberg, J Buell, CR Douches, DS AF Hardigan, Michael A. Bamberg, John Buell, C. Robin Douches, David S. TI Taxonomy and Genetic Differentiation among Wild and Cultivated Germplasm of Solanum sect. Petota SO PLANT GENOME LA English DT Article ID BREVICAULE COMPLEX SOLANACEAE; ENDOSPERM BALANCE NUMBER; POLYCOMB-GROUP PROTEINS; SPECIES BOUNDARIES; ARABIDOPSIS-THALIANA; CROP DOMESTICATION; POTATO; DIVERSITY; SUCROSE; STARCH AB Because of their adaptation to a diverse set of habitats and stresses, wild species of cultivated crops offer new sources of genetic diversity for germplasm improvement. Using an Infinium array representing a genome-wide set of 8303 single nucleotide polymorphisms (SNPs), phylogenetic relationships and allelic diversity were evaluated within a diversity panel of germplasm from Solanum sect. Petota. This panel consists of 74 plant introductions (PIs) representing 25 species and provides a diverse representation of tuber-bearing Solanum germplasm. Unlike other molecular markers, genome-wide SNPs have not been widely implemented in potato. To determine relatedness between current species classifications and SNP-based genetic distances, a phylogeny was generated based on random individuals from each core collection PI. With few exceptions, SNP-based estimates of species relationships revealed general agreement with the existing taxonomic grouping of species in Solanum sect. Petota. Genotype comparisons between the Solanum sect. Petota diversity panel and a panel of 213 tetraploid cultivars and breeding lines indicated a greater extent of diversity between populations of native Andean landraces than among modern cultivated varieties. Comparison of SNP allele frequencies between the Solanum sect. Petota panel and tetraploid cultivars identified loci with extreme divergence between cultivated potato and its tuber-bearing relatives. Several of these loci are associated with genes related to carbohydrate metabolism and tuber development, suggesting potential roles in potato domestication. The Infinium SNP data offer a new taxonomic view of potato germplasm, while further identifying candidate alleles likely to differentiate wild germplasm and cultivated potato, possibly underlying key agronomic traits. C1 [Hardigan, Michael A.; Buell, C. Robin] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Bamberg, John] USDA ARS, Sturgeon Bay, WI 54235 USA. [Douches, David S.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Douches, DS (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. EM douchesd@msu.edu FU USDA NIFA Award [2009-85606-05673]; Michigan State University FX Funds for this work were provided through an award to DSD and CRB from USDA NIFA Award 2009-85606-05673 and through funds from Michigan State University. NR 70 TC 4 Z9 4 U1 7 U2 32 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.06.0025 PG 16 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900002 ER PT J AU Hart, JP Griffiths, PD AF Hart, John P. Griffiths, Phillip D. TI Genotyping-by-Sequencing Enabled Mapping and Marker Development for the By-2 Potyvirus Resistance Allele in Common Bean SO PLANT GENOME LA English DT Article ID YELLOW-MOSAIC-VIRUS; GENOME-WIDE ASSOCIATION; BREEDING LINE RESISTANT; PHASEOLUS-VULGARIS; VEIN-VIRUS; GENE; STRAIN; POPULATIONS; INHERITANCE; MANAGEMENT AB Since its emergence in 2001, an aphid-transmitted virus disease complex has caused substantial economic losses to snap bean (Phaseolus vulgaris L.) production and processing in the Great Lakes Region of the United States. The general ineffectiveness of chemical control measures for nonpersistently transmitted viruses established an urgent need for the development and deployment of cultivars with resistance to the component viruses. Our objectives were to further characterize the inheritance of resistance to Bean yellow mosaic virus (BYMV), which is conditioned by the By-2 allele, to adapt genotyping-by-sequencing (GBS) to common bean to discover and genotype genome-wide single nucleotide polymorphisms (SNPs) in a set of recombinant inbred lines (RILs) derived from an introgression program, and to enable and validate marker-assisted selection for By-2. We optimized ApeKI for GBS in common bean and retained 7530 high-quality SNPs that segregated in our introgression RILs. A case-control genome-wide association study (GWAS) was used to discover 44 GBS SNPs that were strongly associated with the resistance phenotype and which delimited a 974 kb physical interval on the distal portion of chromosome 2. Seven of these SNPs were converted to single-marker Kompetitive Allele-Specific Polymerase chain reaction (KASP) assays and were demonstrated to be tightly linked to BYMV resistance in an F-2 population of 185 individuals. This research enables marker-assisted selection of By-2, provides enhanced resolution for fine mapping, and demonstrates the potential of GBS as a highly efficient, high-throughput genotyping platform for common bean breeding and genetics. C1 [Hart, John P.] USDA ARS, TARS, Mayaguez, PR 00680 USA. [Griffiths, Phillip D.] Cornell Univ, Plant Breeding & Genet Sect, Sch Integrat Plant Sci, Geneva, NY 14456 USA. RP Hart, JP (reprint author), USDA ARS, TARS, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. EM jph248@cornell.edu FU New York State Vegetable Research Association; Seneca Foods Corporation; DuPont Pioneer gift; Cornell Graduate School; Bullis Fellowship of the New York State Agricultural Experiment Station FX We thank Lisa Blanchard, Charlotte Acharya, Rob Elshire, and Sharon Mitchell for sharing their expertise for GBS library construction, and we wish to acknowledge the expertise of Jenny Xiang and the Weill Cornell Medical College Genomics Resources Core Facility. We thank Jud Ward for bioinformatics support and for reviewing the manuscript and we also thank Tim Porch for reviewing the manuscript. We thank Kelly Swarts for providing key support with FILLIN SNP imputation. We thank Alex Lipka for key support with GAPIT and GWAS and we acknowledge the valuable insight of Jason Peiffer, Michael Gore, and Michael Mazourek in reviewing the results. Matthew Wavrick, Sarah Durkee, Jeff McNamara, and Kevin McGuigan provided excellent technical support. This research was supported through funding from the New York State Vegetable Research Association and the Seneca Foods Corporation. JH was provided with fellowship support from a DuPont Pioneer gift to Cornell University's Department of Plant Breeding and Genetics, the Cornell Graduate School, and the Bullis Fellowship of the New York State Agricultural Experiment Station. NR 70 TC 5 Z9 5 U1 2 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.09.0058 PG 14 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900012 ER PT J AU Islam, MS Thyssen, GN Jenkins, JN Fang, DD AF Islam, M. Sariful Thyssen, Gregory N. Jenkins, Johnie N. Fang, David D. TI Detection, Validation, and Application of Genotyping-by-Sequencing Based Single Nucleotide Polymorphisms in Upland Cotton SO PLANT GENOME LA English DT Article ID GENOME SEQUENCE; DIVERSITY; L.; DISCOVERY; MARKERS; ASSAYS; READS AB The presence of two closely related subgenomes in the allotetraploid Upland cotton, combined with a narrow genetic base of the cultivated varieties, has hindered the identification of polymorphic genetic markers and their use in improving this important crop. Genotyping-by-sequencing (GBS) is a rapid way to identify single nucleotide polymorphism (SNP) markers; however, these SNPs may be specific to the sequenced cotton lines. Our objective was to obtain a large set of polymorphic SNPs with broad applicability to the cultivated cotton germplasm. We selected 11 diverse cultivars and their random-mated recombinant inbred progeny for SNP marker development via GBS. Two different GBS methodologies were used by Data2Bio (D2B) and the Institute for Genome Diversity (IGD) to identify 4441 and 1176 polymorphic SNPs with minor allele frequency of >= 0.1, respectively. We further filtered the SNPs and aligned their sequences to the diploid Gossypium raimondii reference genome. We were able to use homeologous SNPs to assign 1071 SNP loci to the At subgenome and 1223 to the Dt subgenome. These filtered SNPs were located in genic regions about twice as frequently as expected by chance. We tested 111 of the SNPs in 154 diverse Upland cotton lines, which confirmed the utility of the SNP markers developed in such approach. Not only were the SNPs identified in the 11 cultivars present in the 154 cotton lines, no two cultivars had identical SNP genotypes. We conclude that GBS can be easily used to discover SNPs in Upland cotton, which can be converted to functional genotypic assays for use in breeding and genetic studies. C1 [Islam, M. Sariful; Thyssen, Gregory N.; Fang, David D.] ARS, USDA, SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. [Jenkins, Johnie N.] ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. [Fang, David D.] ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. RP Fang, DD (reprint author), ARS, USDA, SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459 FU United States Department of Agriculture-Agricultural Research Service CRIS [6435-21000-017-00D, 6406-21000-12-00D]; Cotton Incorporated Project [10-747] FX This research was funded by United States Department of Agriculture-Agricultural Research Service CRIS projects 6435-21000-017-00D, 6406-21000-12-00D, and Cotton Incorporated Project 10-747. We thank Mrs. Ping Li for her help during validation. Our appreciation goes to Data2Bio, LLC, Ames, IA, and Institute for Genome Diversity, Cornell University, for their excellent support in library construction, sequencing, and bioinformatics. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture, which is an equal opportunity provider and employer. NR 34 TC 4 Z9 4 U1 7 U2 33 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.07.0034 PG 10 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900006 ER PT J AU Rutkoski, J Singh, RP Huerta-Espino, J Bhavani, S Poland, J Jannink, JL Sorrells, ME AF Rutkoski, J. Singh, R. P. Huerta-Espino, J. Bhavani, S. Poland, J. Jannink, J. L. Sorrells, M. E. TI Efficient Use of Historical Data for Genomic Selection: A Case Study of Stem Rust Resistance in Wheat SO PLANT GENOME LA English DT Article ID POPULATION-STRUCTURE; QUANTITATIVE TRAITS; RIDGE-REGRESSION; INFORMATION; PREDICTION; ACCURACY; IMPACT; MAIZE; INDIVIDUALS; ASSOCIATION AB Genomic selection (GS) is a methodology that can improve crop breeding efficiency. To implement GS, a training population (TP) with phenotypic and genotypic data is required to train a statistical model used to predict genotyped selection candidates (SCs). A key factor impacting prediction accuracy is the relationship between the TP and the SCs. This study used empirical data for quantitative adult plant resistance to stem rust of wheat (Triticum aestivum L.) to investigate the utility of a historical TP (TPH) compared with a population-specific TP (TPPS), the potential for TPH optimization, and the utility of TPH data when close relative data is available for training. We found that, depending on the population size, a TPPS was 1.5 to 4.4 times more accurate than a TPH, and TPH optimization based on the mean of the generalized coefficient of determination or prediction error variance enabled the selection of subsets that led to significantly higher accuracy than randomly selected subsets. Retaining historical data when data on close relatives were available lead to a 11.9% increase in accuracy, at best, and a 12% decrease in accuracy, at worst, depending on the heritability. We conclude that historical data could be used successfully to initiate a GS program, especially if the dataset is very large and of high heritability. Training population optimization would be useful for the identification of TPH subsets to phenotype additional traits. However, after model updating, discarding historical data may be warranted. More studies are needed to determine if these observations represent general trends. C1 [Rutkoski, J.] Cornell Univ, Sch Integrat Plant Sci, Coll Agr & Life Sci, Int Programs, Ithaca, NY 14853 USA. [Rutkoski, J.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Rutkoski, J.; Singh, R. P.; Huerta-Espino, J.] CIMMYT, Int Maize & Wheat Improvement Ctr, El Batan 06600, DF, Mexico. [Huerta-Espino, J.] Campo Expt Valle Mexico INIFAP, Chapingo 56230, Edo De Mexico, Mexico. [Bhavani, S.] CIMMYT, Nairobi, Kenya. [Poland, J.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Manhattan, KS 66506 USA. [Poland, J.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Jannink, J. L.] USDA ARS, Ithaca, NY 14853 USA. [Jannink, J. L.; Sorrells, M. E.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. RP Rutkoski, J (reprint author), Cornell Univ, Sch Integrat Plant Sci, Coll Agr & Life Sci, Int Programs, 240 Emerson Hall, Ithaca, NY 14853 USA. EM jer263@cornell.edu OI bhavani, sridhar/0000-0002-4091-2608; Rutkoski, Jessica/0000-0001-8435-4049; Poland, Jesse/0000-0002-7856-1399 FU Bill and Melinda Gates Foundation; USDA-ARS [5430-21000-006-00D]; USDA National Institute of Food and Agriculture (NIFA)-Agriculture and Food Research Initiative (AFRI) [2011-68002-30029]; United States Agency for International Development (USAID) [AID-OAA-A-13-00051]; USDA National Needs Fellowship Grant [2008-38420-04755]; American Society of Plant Biology (ASPB)-Pioneer Hi-Bred Graduate Student Fellowship; USDA-NIFA [149-430] FX This research was funded by The Bill and Melinda Gates Foundation (grants: Durable Rust Resistance in Wheat and Genomic Selection: The Next Frontier for Rapid Gains in Maize And Wheat Improvement), the USDA-ARS (Appropriation No. 5430-21000-006-00D), USDA National Institute of Food and Agriculture (NIFA)-Agriculture and Food Research Initiative (AFRI) grant support award number 2011-68002-30029, and United States Agency for International Development (USAID) support to the Feed the Future Innovation Lab for Applied Wheat Genomics (Cooperative Agreement No. AID-OAA-A-13-00051). Partial support for J. Rutkoski was provided by a USDA National Needs Fellowship Grant #2008-38420-04755 and an American Society of Plant Biology (ASPB)-Pioneer Hi-Bred Graduate Student Fellowship. Partial support was also provided by USDA-NIFA Hatch Project 149-430. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 40 TC 6 Z9 6 U1 5 U2 21 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.09.0046 PG 10 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900010 ER PT J AU Sallam, AH Endelman, JB Jannink, JL Smith, KP AF Sallam, A. H. Endelman, J. B. Jannink, J. -L. Smith, K. P. TI Assessing Genomic Selection Prediction Accuracy in a Dynamic Barley Breeding Population SO PLANT GENOME LA English DT Article ID FUSARIUM HEAD BLIGHT; MARKER-ASSISTED SELECTION; HILBERT-SPACES REGRESSION; QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; GENETIC ARCHITECTURE; MOLECULAR MARKERS; RIDGE-REGRESSION; COMPLEX TRAITS; F-STATISTICS AB Prediction accuracy of genomic selection (GS) has been previously evaluated through simulation and cross-validation; however, validation based on progeny performance in a plant breeding program has not been investigated thoroughly. We evaluated several prediction models in a dynamic barley breeding population comprised of 647 six-row lines using four traits differing in genetic architecture and 1536 single nucleotide polymorphism (SNP) markers. The breeding lines were divided into six sets designated as one parent set and five consecutive progeny sets comprised of representative samples of breeding lines over a 5-yr period. We used these data sets to investigate the effect of model and training population composition on prediction accuracy over time. We found little difference in prediction accuracy among the models confirming prior studies that found the simplest model, random regression best linear unbiased prediction (RR-BLUP), to be accurate across a range of situations. In general, we found that using the parent set was sufficient to predict progeny sets with little to no gain in accuracy from generating larger training populations by combining the parent set with subsequent progeny sets. The prediction accuracy ranged from 0.03 to 0.99 across the four traits and five progeny sets. We explored characteristics of the training and validation populations (marker allele frequency, population structure, and linkage disequilibrium, LD) as well as characteristics of the trait (genetic architecture and heritability, H-2). Fixation of markers associated with a trait over time was most clearly associated with reduced prediction accuracy for the mycotoxin trait DON. Higher trait H-2 in the training population and simpler trait architecture were associated with greater prediction accuracy. C1 [Sallam, A. H.; Smith, K. P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Endelman, J. B.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Jannink, J. -L.] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Smith, KP (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM smith376@umn.edu FU National Institute of Food and Agriculture USDA [2009-65300-05661]; U.S. Wheat and Barley Scab Initiative USDA-ARS [59-0206-9-072]; USDA HATCH project [MIN-13-030]; Rahr Foundation FX We thank Ed Schiefelbein, Guillermo Velasquez, Karen Beaubien, Richard Horsley, the Minnesota Agricultural Experiment Station NW and WC Research and Outreach Centers, and the University of Minnesota Small Grains Pathology Lab for their contributions to conducting field trials and collecting data. In addition, we thank Shiaoman Chao and Yanhong Dong for SNP genotyping and toxin analysis, respectively. Additional thanks to Yang Da for helpful suggestions on an earlier draft of this manuscript. Funding for this work was supported by grants from the National Institute of Food and Agriculture USDA Award Number 2009-65300-05661, the U.S. Wheat and Barley Scab Initiative USDA-ARS Agreement No. 59-0206-9-072, USDA HATCH project MIN-13-030, and the Rahr Foundation. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 76 TC 11 Z9 11 U1 2 U2 29 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.05.0020 PG 15 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900001 ER PT J AU Vincent, JA Stacey, M Stacey, G Bilyeu, KD AF Vincent, Jennifer A. Stacey, Minviluz Stacey, Gary Bilyeu, Kristin D. TI Phytic Acid and Inorganic Phosphate Composition in Soybean Lines with Independent IPK1 Mutations SO PLANT GENOME LA English DT Article ID INOSITOL 1,3,4,5,6-PENTAKISPHOSPHATE 2-KINASE; GLYCINE-MAX; MODIFIED PHOSPHORUS; FIELD EMERGENCE; PHYTATE; ARABIDOPSIS; GENERATION; PHENOTYPE; SELECTION; GENETICS AB Soybean seeds contain a large amount of P, which is stored as phytic acid (PA). Phytic acid is indigestible by nonruminant livestock and considered an antinutritional factor in soybean meal. Several low PA soybean lines have been discovered, but many of these lines have either minor reductions in PA or inadequate germination and emergence. The reduced PA phenotype of soybean line Gm-lpa-ZC-2 was previously shown to be the result of a mutation in a gene encoding an inositol pentakisphosphate 2-kinase on chromosome 14 (14IPK1). While the 14IPK1 mutation was shown to have no impact on germination and emergence, the reduction in PA was modest (up to 50%). Our objective was to determine the effect on seed P partitioning for a novel mutation of an independent IPK1 gene on chromosome six (06IPK1) on its own and in combination with mutant alleles of the 14IPK1. We developed soybean populations and conducted genotype and phenotype association analyses based on the genotype of the 06IPK1 and 14IPK1 genes and the seed P partitioning profile. The lines with both mutant IPK1 genes had very low PA levels, moderate accumulation of inorganic phosphate (Pi), and accumulation of high amounts of P in lower inositols. The developed lines did not have significant reductions in germination or field emergence. In addition, characterization of the lower inositols produced in the mutant lines suggests that IPK1 is a polyphosphate kinase and provides some insight into the PA biosynthesis pathway in soybean seeds. C1 [Vincent, Jennifer A.; Stacey, Minviluz; Stacey, Gary; Bilyeu, Kristin D.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Bilyeu, Kristin D.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Bilyeu, KD (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM Kristin.Bilyeu@ars.usda.gov FU United Soybean Board FX We would like to thank the Paul Little and Christi Cole for their superb technical assistance. This research was supported by the United Soybean Board. We would also like to thank Pengyin Chen (University of Arkansas) for his generous sharing of Gm-lpa-ZC-2 seed. NR 36 TC 1 Z9 1 U1 4 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.10.0077 PG 10 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900016 ER PT J AU Wallace, JG Upadhyaya, HD Vetriventhan, M Buckler, ES Hash, CT Ramu, P AF Wallace, Jason G. Upadhyaya, Hari D. Vetriventhan, M. Buckler, Edward S. Hash, C. Tom Ramu, Punna TI The Genetic Makeup of a Global Barnyard Millet Germplasm Collection SO PLANT GENOME LA English DT Article ID POPULATION-STRUCTURE; ORPHAN CROPS; DNA AMOUNTS; INFERENCE; QUALITY; SAMPLES AB Barnyard millet (Echinochloa spp.) is an important crop for many smallholder farmers in southern and eastern Asia. It is valued for its drought tolerance, rapid maturation, and superior nutritional qualities. Despite these characteristics there are almost no genetic or genomic resources for this crop in either cultivated species [E. colona (L.) Link and E. crus-galli (L.) P. Beauv.]. Recently, a core collection of 89 barnyard millet accessions was developed at the genebank at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). To enhance the use of this germplasm and genomic research in barnyard millet improvement, we report the genetic characterization of this core collection using whole-genome genotyping-by-sequencing. We identified several thousand single-nucleotide polymorphisms segregating in the core collection, and we use them to show patterns of population structure and phylogenetic relationships among the accessions. We determine that there are probably four population clusters within the E. colona accessions and three such clusters within E. crus-galli. These clusters match phylogenetic relationships but by and large do not correspond to classification into individual races or clusters based on morphology. Geospatial data available for a subset of samples indicates that the clusters probably originate from geographic divisions. In all, these data will be useful to breeders working to improve this crop for smallholder farmers. This work also serves as a case study of how modern genomics can rapidly characterize crops, including ones with little to no prior genetic data. C1 [Wallace, Jason G.; Buckler, Edward S.; Ramu, Punna] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. [Upadhyaya, Hari D.; Vetriventhan, M.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Telangan, India. [Hash, C. Tom] ISC, ICRISAT Sahelian Ctr, Niamey, Niger. RP Wallace, JG (reprint author), Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. EM jason.wallace@cornell.edu RI Updhayaya, Hari/C-4858-2014; OI Updhayaya, Hari/0000-0002-5166-6844; Wallace, Jason/0000-0002-8937-6543 FU NSF [DBI-0820619, IOS-1238014]; ICRISAT; USDA-ARS FX This work was supported by NSF grants DBI-0820619 and IOS-1238014, ICRISAT, and the USDA-ARS. This work has been undertaken as part of the CGIAR Research Program on Dryland Cereals. Punna Ramu, Hari D. Upadhyaya, and M. Vetriventhan selected materials and prepared the samples for analysis. Jason G. Wallace performed bioinformatic analyses and had primary responsibility for writing the paper. C. Tom Hash and Edward S. Buckler provided oversight and direction. All authors had responsibility for editing the manuscript for publication. NR 31 TC 3 Z9 3 U1 2 U2 15 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.10.0067 PG 7 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900014 ER PT J AU Zhao, MX Cai, CM Zhai, JX Lin, F Li, LH Shreve, J Thimmapuram, J Hughes, TJ Meyers, BC Ma, JX AF Zhao, Meixia Cai, Chunmei Zhai, Jixian Lin, Feng Li, Linghong Shreve, Jacob Thimmapuram, Jyothi Hughes, Teresa J. Meyers, Blake C. Ma, Jianxin TI Coordination of MicroRNAs, PhasiRNAs, and NB-LRR Genes in Response to a Plant Pathogen: Insights from Analyses of a Set of Soybean Rps Gene Near-Isogenic Lines SO PLANT GENOME LA English DT Article ID TRANS-ACTING SIRNAS; SMALL INTERFERING RNAS; PHYTOPHTHORA-SOJAE; RESISTANCE GENES; DNA METHYLATION; MESSENGER-RNAS; GLYCINE-MAX; ARABIDOPSIS; BIOGENESIS; SECONDARY AB Disease-related genes, particularly the nucleotide binding site (NB)-leucine-rich repeat (LRR) class of R plant genes can be triggered by microRNAs (miRNAs) to generate phased small interfering RNAs (phasiRNAs), which could reduce the transcript levels of their targets. However, how global changes in NB-LRR transcript levels coordinate with changes in miRNA and phasiRNA levels in defense responses remains largely unknown. Here, we investigated changes in the relative abundance of small RNAs (sRNAs), with a focus on miRNAs and phasiRNAs and their potential targets in response to the pathogen Phytophthora sojae in the susceptible soybean [Glycine max (L.) Merr.] 'Williams' and nine resistant near-isogenic lines (NILs), each carrying a unique resistance to P. sojae (Rps) gene. In total, 369 distinct miRNAs, including 78 new ones, were identified in the 10 soybean lines. The majority of miRNAs were downregulated by the pathogen. Of the 525 NB-LRR genes found in the soybean reference genome, 257 were predicted to be the targets of eight abundant miRNA families and 126 (dubbed phasi-NB-LRRs or pNLs) were predicted to have produced phasiRNAs. Upregulation of 15 phasi-NB-LRRs was associated with downregulation of their corresponding phasiRNAs in the NILs; these phasiRNAs were predicted to regulate 75 additional NB-LRRs in trans. In addition, we identified putative 24-nucleotide (nt) phasiRNAs from transposons, possibly representing a novel general epigenetic mechanism for regulation of transposon activity under biotic stresses. Together, these observations suggest that miRNAs and phasiRNAs play an important role in response to plant pathogens through complex, multiple layers of post-transcriptional regulation. C1 [Zhao, Meixia; Lin, Feng; Ma, Jianxin] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Cai, Chunmei; Li, Linghong; Ma, Jianxin] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China. [Zhai, Jixian; Meyers, Blake C.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Zhai, Jixian; Meyers, Blake C.] Univ Delaware, Delaware Biotechnol Inst, Newark, DE 19716 USA. [Shreve, Jacob; Thimmapuram, Jyothi] Purdue Univ, Bioinformat Core Facil, W Lafayette, IN 47907 USA. [Hughes, Teresa J.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Hughes, Teresa J.] Monsanto Co, St Louis, MO 63167 USA. RP Ma, JX (reprint author), Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. EM maj@purdue.edu RI ZHAO, MEIXIA/N-3124-2015; Meyers, Blake/B-6535-2012; Lin, Feng/K-1814-2016 OI ZHAO, MEIXIA/0000-0001-8812-8217; Meyers, Blake/0000-0003-3436-6097; Lin, Feng/0000-0001-7491-4444 FU Indiana Soybean Alliance [205267]; National Natural Science Foundation of China [31371647]; Taishan Scholarship and High Level Talents Foundation of Qingdao Agricultural University [631304] FX We thank Phillip SanMiguel for assistance in sRNA sequencing and Doug Yatcilla for help with software installation and testing. This work was mainly supported by soybean check-off funds from the Indiana Soybean Alliance (grant number 205267) and partially supported by funds from the National Natural Science Foundation of China (grant number 31371647) and the Taishan Scholarship and High Level Talents Foundation of Qingdao Agricultural University (grant number 631304). NR 67 TC 4 Z9 4 U1 2 U2 20 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2015 VL 8 IS 1 DI 10.3835/plantgenome2014.09.0044 PG 13 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CF4CA UT WOS:000352494900009 ER PT J AU Bieren, JV Volpe, B Sutherland, DB Burgi, J Verbeek, JS Marsland, BJ Urban, JF Harris, NL AF Esser-von Bieren, Julia Volpe, Beatrice Sutherland, Duncan B. Buergi, Jerome Verbeek, J. Sjef Marsland, Benjamin J. Urban, Joseph F., Jr. Harris, Nicola L. TI Immune Antibodies and Helminth Products Drive CXCR2-Dependent Macrophage-Myofibroblast Crosstalk to Promote Intestinal Repair SO PLOS PATHOGENS LA English DT Article ID PROTECTIVE IMMUNITY; DENDRITIC CELLS; RESPONSES; MICE; RECEPTOR; CXCR2; ACTIVATION; EXPRESSION; INNATE; INFLAMMATION AB Helminth parasites can cause considerable damage when migrating through host tissues, thus making rapid tissue repair imperative to prevent bleeding and bacterial dissemination particularly during enteric infection. However, how protective type 2 responses targeted against these tissue-disruptive multicellular parasites might contribute to homeostatic wound healing in the intestine has remained unclear. Here, we observed that mice lacking antibodies (Aid(-/-)) or activating Fc receptors (Fcrg(-/-)) displayed impaired intestinal repair following infection with the murine helminth Heligmosomoides polygyrus bakeri (Hpb), whilst transfer of immune serum could partially restore chemokine production and rescue wound healing in Aid(-/-) mice. Impaired healing was associated with a reduced expression of CXCR2 ligands (CXCL2/3) by macrophages (M phi) and myofibroblasts (MF) within intestinal lesions. Whilst antibodies and helminths together triggered CXCL2 production by MF in vitro via surface FcR engagement, chemokine secretion by intestinal MF was elicited by helminths directly via Fcrg-chain/dectin2 signaling. Blockade of CXCR2 during Hpb challenge infection reproduced the delayed wound repair observed in helminth infected Aid(-/-) and Fcrg(-/-) mice. Finally, conditioned media from human MF stimulated with infective larvae of the helminth Ascaris suum together with immune serum, promoted CXCR2-dependent scratch wound closure by human MF in vitro. Collectively our findings suggest that helminths and antibodies instruct a chemokine driven MF-MF crosstalk to promote intestinal repair, a capacity that may be harnessed in clinical settings of impaired wound healing. C1 [Esser-von Bieren, Julia; Volpe, Beatrice; Sutherland, Duncan B.; Harris, Nicola L.] Ecole Polytech Fed Lausanne, Sch Life Sci, Global Hlth Inst, Lab Intestinal Immunol, Lausanne, Switzerland. [Esser-von Bieren, Julia] Tech Univ Munich, Ctr Allergy & Environm ZAUM, D-80290 Munich, Germany. [Esser-von Bieren, Julia] Helmholtz Ctr Munich, Munich, Germany. [Buergi, Jerome] Ecole Polytech Fed Lausanne, Sch Life Sci, Global Hlth Inst, Lab Cell & Membrane Biol, Lausanne, Switzerland. [Verbeek, J. Sjef] Leiden Univ, Med Ctr, Dept Human Genet, Leiden, Netherlands. [Marsland, Benjamin J.] Univ Lausanne, Fac Biol & Med, Serv Pneumol, CHU Vaudois, Lausanne, Switzerland. [Urban, Joseph F., Jr.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. RP Bieren, JV (reprint author), Ecole Polytech Fed Lausanne, Sch Life Sci, Global Hlth Inst, Lab Intestinal Immunol, Lausanne, Switzerland. EM nicola.harris@epfl.ch OI Urban, Joseph/0000-0002-1590-8869 FU Swiss National Science Foundation [SNF310030_133104] FX This study was supported by by the Swiss National Science Foundation (SNF310030_133104). http://www.snf.ch/en/Pages/default.aspx. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 1 Z9 1 U1 3 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD MAR PY 2015 VL 11 IS 3 AR UNSP e1004778 DI 10.1371/journal.ppat.1004778 PG 23 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA CE9ZI UT WOS:000352201900081 ER PT J AU Wang, QQ Zhu, JY AF Wang, Qianqian Zhu, J. Y. TI Facile preparation of nanofiller-paper using mixed office paper without deinking SO TAPPI JOURNAL LA English DT Review ID CELLULOSE NANOFIBRILS; BARRIER PROPERTIES; NANOCELLULOSES; NANOCOMPOSITES; STIFF AB Mixed office paper (MOP) pulp without deinking with an ash content of 18.1 +/- 1.5% was used as raw material to produce nanofiller-paper. The MOP pulp with filler was mechanically fibrillated using a laboratory stone grinder. Scanning electron microscope imaging revealed that the ground filler particles were wrapped by cellulose nanofibrils (CNFs), which substantially improved the incorporation of filler into the CNF matrix. Sheets made of this CNF matrix were densified due to improved bonding. Specific tensile strength and modulus of the nanofiller-paper with 60-min grinding reached 48.4 kN.m/kg and 8.1 MN.m/kg, respectively, approximately 250% and 200% of the respective values of the paper made of unground MOP pulp. Mechanical grinding duration did not affect the thermal stability of the nanofiller-paper. C1 [Wang, Qianqian] Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang, Peoples R China. [Zhu, J. Y.] USDA, Forest Serv, Forest Prod Lab, Madison, WI USA. RP Wang, QQ (reprint author), Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang, Peoples R China. EM jzhu@fs.fed.us RI Wang, Qianqian/G-1915-2012 OI Wang, Qianqian/0000-0003-3514-455X FU Natural Science Foundation of China [31300493]; USDA Agriculture and Food Research Initiative (AFRI) Competitive Program [2011-67009-20056] FX The authors gratefully acknowledge the financial support from the Natural Science Foundation of China (Grant No. 31300493) and the USDA Agriculture and Food Research Initiative (AFRI) Competitive Program (No. 2011-67009-20056). NR 31 TC 3 Z9 3 U1 2 U2 5 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD MAR PY 2015 VL 14 IS 3 BP 167 EP 174 PG 8 WC Materials Science, Paper & Wood SC Materials Science GA CF4VV UT WOS:000352551900003 ER PT J AU Starbuck, CA Amelon, SK Thompson, FR AF Starbuck, Clarissa A. Amelon, Sybill K. Thompson, Frank R., III TI Relationships Between Bat Occupancy and Habitat and Landscape Structure Along a Savanna, Woodland, Forest Gradient in the Missouri Ozarks SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE big brown bat; eastern red bat; evening bat; northern long-eared bat; restoration; site occupancy; tri-colored bat ID LONG-EARED BATS; WIND ENERGY FACILITIES; EASTERN RED BATS; NORTH-AMERICA; MYOTIS-SEPTENTRIONALIS; LASIURUS-BOREALIS; EPTESICUS-FUSCUS; ROOSTING ECOLOGY; SOUTH-CAROLINA; SITE OCCUPANCY AB Many land-management agencies are restoring savannas and woodlands using prescribed fire and forest thinning, and information is needed on how wildlife species respond to these management activities. Our objectives were to evaluate support for relationships of bat site occupancy with vegetation structure and management and landscape composition and structure across a gradient of savanna to forest in the Missouri Ozark Highlands, USA. We selected study sites that were actively managed for savanna and woodland conditions and control areas on similar landforms that had succeeded to closed-canopy forest. We used Anabat detectors to survey bats during the summers of 2010, 2011, and 2012. We fit single-species site-occupancy models to estimate detection probability and site occupancy. We evaluated a priori hypotheses in an information theoretic approach by evaluating support for candidate models that included habitat, landscape, and management effects. Site occupancy of evening bats (Nycticeius humeralis) was negatively related to poletimber and sawtimber density and positively related to fire frequency, while northern long-eared bat (Myotis septentrionalis) site occupancy was positively related to poletimber density and negatively related to understory stem densities. Site occupancy of big brown bats (Eptesicus fuscus), eastern red bats (Lasiurus borealis), and tri-colored bats (Perimyotis subflavus) were mostly not related to local vegetation structure and site occupancy was high across the savanna, woodland, forest gradient. We found more consistent and larger effect sizes for landscape-scale than for habitat-scale relationships; therefore, land managers should be cognizant of large-scale patterns in land cover when making local management decisions for these species. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Starbuck, Clarissa A.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Amelon, Sybill K.; Thompson, Frank R., III] Univ Missouri, USDA ARS, No Res Stn, Columbia, MO 65211 USA. RP Starbuck, CA (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM cas726@nau.edu OI Amelon, Sybill/0000-0003-3940-8153 FU U.S. Department of Agriculture Forest Service Northern Research Station FX We thank W. Dijak, J. Amelon, S. Kendrick, and J. Reidy for help with this study. We thank the U.S. Department of Agriculture Forest Service Northern Research Station for funding this project. The Mark Twain National Forest, Missouri Department of Conservation, and Missouri Department of Natural Resources provided study sites, information on the management of sites, and other logistical support. We thank an anonymous reviewer, L. Dodd, Associate Editor E. Arnett, and Editor C. Ribic for helpful comments on the manuscript. NR 50 TC 2 Z9 2 U1 16 U2 70 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD MAR PY 2015 VL 39 IS 1 BP 20 EP 30 DI 10.1002/wsb.512 PG 11 WC Biodiversity Conservation SC Biodiversity & Conservation GA CE8MA UT WOS:000352095300003 ER PT J AU Beasley, J Webster, SC Rhodes, OE Cunningham, FL AF Beasley, James Webster, Sarah C. Rhodes, Olin E., Jr. Cunningham, Fred L. TI Evaluation of Rhodamine B as a Biomarker for Assessing Bait Acceptance in Wild Pigs SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE bait; boar; feral swine; hair; pharmaceutical; Rhodamine B; Sus scrofa; toxicant; vaccination; wild pig ID CLASSICAL SWINE-FEVER; FERAL SWINE; ORAL IMMUNIZATION; SUS-SCROFA; MARKER; BOAR; POPULATIONS; AUSTRALIA; PRESSURE; EFFICACY AB Worldwide, there is growing interest in the use of pharmaceutical baits to control populations of wild pigs (Sus scrofa). In this study we evaluated the utility of Rhodamine B (RB), a chemical marker commonly used in wildlife research and management, as a potential biomarker for quantifying bait uptake in wild pigs. Thirty wild pigs were live-trapped, transported to a captive facility on the Department of Energy's Savannah River Site located in South Carolina, USA, during autumn 2013, and administered RB orally at a dosage of 30mg/kg. Eight vibrissae and guard hairs were collected pre- and post-RB exposure (7 or 14 days) and evaluated for the presence of RB using fluorescence microscopy. No evidence of RB marking was observed in any samples collected pre-RB administration. In contrast, we observed fluorescent marking post-RB exposure that was indicative of the presence of RB for all individuals, with 98% of vibrissae and 100% of guard hairs exhibiting RB marks. The uniform detection of RB among individuals and consistent manifestation of marks in both guard hair and vibrissae, samples that easily can be collected and stored by untrained field personnel from live or deceased pigs, suggests that RB is an effective biomarker for use in large-scale management programs to control wild pigs. In particular, our results, combined with previous studies evaluating uptake of RB in other species, suggest that RB can be used to develop baiting programs to deliver pharmaceuticals to free-ranging wild pigs, as well as evaluate the potential impacts of pig baits on non-target species. (c) 2014 The Wildlife Society. C1 [Beasley, James; Webster, Sarah C.; Rhodes, Olin E., Jr.] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA. [Cunningham, Fred L.] USDA, Anim Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, Mississippi State, MS 39762 USA. RP Beasley, J (reprint author), Univ Georgia, Savannah River Ecol Lab, PO Drawer E, Aiken, SC 29802 USA. EM beasley@srel.uga.edu FU U.S. Department of Agriculture National Wildlife Research Center; U.S. Department of Energy [DE-FC09-07SR22506] FX We would especially like to thank T. Grazia, J. Nance, T. Mims, and the Savannah River Site pig-trapping contractors for their assistance capturing wild pigs for this study. We also would like to thank Z. Smith and L. Oliver for their assistance with this research and 2 anonymous reviewers for providing comments on this manuscript. Funding was provided by the U.S. Department of Agriculture National Wildlife Research Center and the U.S. Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. NR 33 TC 1 Z9 1 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD MAR PY 2015 VL 39 IS 1 BP 188 EP 192 DI 10.1002/wsb.510 PG 5 WC Biodiversity Conservation SC Biodiversity & Conservation GA CE8MA UT WOS:000352095300023 ER PT J AU Jiang, XQ Yu, Y Chen, JW Zhao, MK Chen, H Song, XZ Matzuk, AJ Carroll, SL Tan, X Sizovs, A Cheng, NH Wang, MC Wang, J AF Jiang, Xiqian Yu, Yong Chen, Jianwei Zhao, Mingkun Chen, Hui Song, Xianzhou Matzuk, Alexander J. Carroll, Shaina L. Tan, Xiao Sizovs, Antons Cheng, Ninghui Wang, Meng C. Wang, Jin TI Quantitative Imaging of Glutathione in Live Cells Using a Reversible Reaction-Based Ratiometric Fluorescent Probe SO ACS CHEMICAL BIOLOGY LA English DT Article ID HIGHLY SELECTIVE DETECTION; HYDROGEN-SULFIDE; LIVING CELLS; IN-VIVO; H2S PRODUCTION; NITRIC-OXIDE; REDOX; THIOLS; MITOCHONDRIA; QUANTIFICATION AB Glutathione (GSH) plays an important role in maintaining redox homeostasis inside cells. Currently, there are no methods available to quantitatively assess the GSH concentration in live cells. Live cell fluorescence imaging revolutionized the field of cell biology and has become an indispensable tool in current biological studies. In order to minimize the disturbance to the biological system in live cell imaging, the probe concentration needs to be significantly lower than the analyte concentration. Because of this, any irreversible reaction-based GSH probe can only provide qualitative results within a short reaction time and will exhibit maximum response regardless of the GSH concentration if the reaction is completed. A reversible reaction-based probe with an appropriate equilibrium constant allows measurement of an analyte at much higher concentrations and, thus, is a prerequisite for GSH quantification inside cells. In this contribution, we report the first fluorescent probe-ThiolQuant Green (TQ Green)-for quantitative imaging of GSH in live cells. Due to the reversible nature of the reaction between the probe and GSH, we are able to quantify mM concentrations of GSH with TQ Green concentrations as low as 20 nM. Furthermore, the GSH concentrations measured using TQ Green in 3T3-L1, HeLa, HepG2, PANC-1, and PANC-28 cells are reproducible and well correlated with the values obtained from cell lysates. TQ Green imaging can also resolve the changes in GSH concentration in PANC-1 cells upon diethylmaleate (DEM) treatment. In addition, TQ Green can be conveniently applied in fluorescence activated cell sorting (FACS) to measure GSH level changes. Through this study, we not only demonstrate the importance of reaction reversibility in designing quantitative reaction-based fluorescent probes but also provide a practical tool to facilitate redox biology studies. C1 [Jiang, Xiqian; Chen, Jianwei; Zhao, Mingkun; Chen, Hui; Song, Xianzhou; Matzuk, Alexander J.; Carroll, Shaina L.; Tan, Xiao; Sizovs, Antons; Wang, Jin] Baylor Coll Med, Dept Pharmacol, Houston, TX 77030 USA. [Yu, Yong; Wang, Meng C.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Yu, Yong; Wang, Meng C.] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Zhao, Mingkun] Baylor Coll Med, Integrat Mol & Biomed Sci Grad Program, Houston, TX 77030 USA. [Cheng, Ninghui] Baylor Coll Med, ARS, USDA, Children Nutr Res Ctr, Houston, TX 77030 USA. [Cheng, Ninghui] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Wang, Jin] Baylor Coll Med, Dan L Duncan Canc Ctr, Ctr Drug Discovery, Houston, TX 77030 USA. [Wang, Jin] Baylor Coll Med, Cardiovasc Res Inst, Houston, TX 77030 USA. RP Wang, J (reprint author), Baylor Coll Med, Dept Pharmacol, Houston, TX 77030 USA. EM wangj@bcm.edu RI Jiang, Xiqian/C-7877-2017; OI Wang, Jin/0000-0003-3625-7919 FU Cancer Prevention and Research Institute of Texas (CPRIT) [R1104]; Welch Foundation [Q-1798]; National Institute of Health [R01AG045183]; Texas Advanced Computing Center (TACC); Center for Drug Discovery at Baylor College of Medicine; Dan L. Duncan Cancer Center; Cytometry and Cell Sorting Core at Baylor College of Medicine; NIH [P30 AI036211, P30 CA125123, S10 RR024574]; Integrative Molecular and Biomedical Sciences Graduate Program Training Grant (NIH) [5 T32 GM008231] FX This work is supported in part by the Cancer Prevention and Research Institute of Texas (CPRIT R1104) and the Welch Foundation (Q-1798) to J.W., National Institute of Health (R01AG045183) to M.C.W., Texas Advanced Computing Center (TACC), the Center for Drug Discovery at Baylor College of Medicine, the Dan L. Duncan Cancer Center, the Cytometry and Cell Sorting Core at Baylor College of Medicine with funding from the NIH (P30 AI036211, P30 CA125123, and S10 RR024574) and the expert assistance of Joel M. Sederstrom, the John S. Dunn Gulf Coast Consortium for Chemical Genomics, and the Shared Equipment Authority instrumentation at Rice University. M.Z. thanks the support from the Integrative Molecular and Biomedical Sciences Graduate Program Training Grant (NIH 5 T32 GM008231). We also thank Dr. S. Ho for sharing pCMV:mRFP-Rab5 plasmid. We also appreciate the discussion and suggestion from the reviewers, Drs. T. Wensel and T. Palzkill at Baylor College of Medicine and Dr. M. Janes and his molecular probe team at Thermo Fisher Scientific Inc. NR 50 TC 9 Z9 9 U1 17 U2 85 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1554-8929 EI 1554-8937 J9 ACS CHEM BIOL JI ACS Chem. Biol. PD MAR PY 2015 VL 10 IS 3 BP 864 EP 874 DI 10.1021/cb500986w PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CE1FY UT WOS:000351558700025 PM 25531746 ER PT J AU Schmidt, WF Broadhurst, CL Qin, JW Lee, H Nguyen, JK Chao, KL Hapeman, CJ Shelton, DR Kim, MS AF Schmidt, Walter F. Broadhurst, C. Leigh Qin, Jianwei Lee, Hoyoung Nguyen, Julie K. Chao, Kuanglin Hapeman, Cathleen J. Shelton, Daniel R. Kim, Moon S. TI Continuous Temperature-Dependent Raman Spectroscopy of Melamine and Structural Analog Detection in Milk Powder SO APPLIED SPECTROSCOPY LA English DT Article DE Raman spectroscopy; Biuret; Cyanuric acid; Melamine; Hyperspectral imaging; Temperature-dependent Raman spectroscopy ID TRANSFORM-INFRARED-SPECTROSCOPY; RARE-EARTH COMPLEXES; RAPID DETECTION; VIBRATIONAL ANALYSIS; IR-SPECTRA; FT-IR; PEPTIDES; NAPHTHALOCYANINE; PHTHALOCYANINE; POLYPEPTIDES AB Hyperspectral Raman imaging has the potential for rapid screening of solid-phase samples for potential adulterants. We can improve mixture analysis algorithms by defining a temperature range in which the contaminant spectrum changes dramatically and uniquely compared with unadulterated material. Raman spectra were acquired for urea, biuret, cyanuric acid, and melamine (pure and at 1% in dried milk powder) from 50 to 310 degrees C with a gradient of 1 degrees C min(-1). Adulterants were clearly indentified in the milk powder. Specific frequencies that were mainly associated with ring breathing, stretching, and in-plane deformation shifted with respect to temperature up to 12 cm(-1) in all four molecules. Specific frequencies significantly increased/decreased in intensity within narrow temperature ranges independent of whether the amine was mixed in milk. Correlation of Raman and differential scanning calorimetry data identified structural components and vibrational modes, which concur with or trigger phase transitions. C1 [Schmidt, Walter F.; Broadhurst, C. Leigh; Qin, Jianwei; Lee, Hoyoung; Nguyen, Julie K.; Chao, Kuanglin; Shelton, Daniel R.; Kim, Moon S.] ARS, Environm Microbiol & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Hapeman, Cathleen J.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Schmidt, WF (reprint author), ARS, Environm Microbiol & Food Safety Lab, USDA, Bldg 303,BARC East,10300 Baltimore Blvd, Beltsville, MD 20705 USA. EM waiter.schmidt@ars.usda.gov NR 38 TC 6 Z9 6 U1 6 U2 21 PU SOC APPLIED SPECTROSCOPY PI FREDERICK PA 5320 SPECTRUM DRIVE SUITE C, FREDERICK, MD 21703 USA SN 0003-7028 EI 1943-3530 J9 APPL SPECTROSC JI Appl. Spectrosc. PD MAR PY 2015 VL 69 IS 3 BP 398 EP 406 DI 10.1366/14-07600 PG 9 WC Instruments & Instrumentation; Spectroscopy SC Instruments & Instrumentation; Spectroscopy GA CE6XT UT WOS:000351982200011 PM 25664966 ER PT J AU Nicastro, HL Ross, SA Milner, JA AF Nicastro, Holly L. Ross, Sharon A. Milner, John A. TI Garlic and Onions: Their Cancer Prevention Properties SO CANCER PREVENTION RESEARCH LA English DT Review ID HELICOBACTER-PYLORI INFECTION; N-ACETYLTRANSFERASE ACTIVITY; FRIED BEEF PATTIES; ORGANOSULFUR COMPOUNDS; GASTRIC-CANCER; DIALLYL DISULFIDE; HETEROCYCLIC AMINES; CELL-PROLIFERATION; ALLIUM VEGETABLES; SULFUR-COMPOUNDS AB The Allium genus includes garlic, onions, shallots, leeks, and chives. These vegetables are popular in cuisines worldwide and are valued for their potential medicinal properties. Epidemiologic studies, while limited in their abilities to assess Allium consumption, indicate some associations of Allium vegetable consumption with decreased risk of cancer, particularly cancers of the gastrointestinal tract. Limited intervention studies have been conducted to support these associations. The majority of supportive evidence on Allium vegetables cancer-preventive effects comes from mechanistic studies. These studies highlight potential mechanisms of individual sulfur-containing compounds and of various preparations and extracts of these vegetables, including decreased bioactivation of carcinogens, antimicrobial activities, and redox modification. Allium vegetables and their components have effects at each stage of carcinogenesis and affect many biologic processes that modify cancer risk. This review discusses the cancer-preventive effects of Allium vegetables, particularly garlic and onions, and their bioactive sulfur compounds and highlights research gaps. (C)2015 AACR. C1 [Nicastro, Holly L.] NCI, Canc Prevent Fellowship Program, Nutr Sci Res Grp, Canc Prevent Div, Bethesda, MD 20892 USA. [Ross, Sharon A.] NCI, Nutr Sci Res Grp, Canc Prevent Div, Bethesda, MD 20892 USA. [Milner, John A.] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. RP Nicastro, HL (reprint author), NHLBI, 9609 Rockledge Dr 10110, Bethesda, MD 20892 USA. EM holly.nicastro@nih.gov FU Intramural NIH HHS [Z99 HL999999] NR 96 TC 9 Z9 9 U1 10 U2 58 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1940-6207 EI 1940-6215 J9 CANCER PREV RES JI Cancer Prev. Res. PD MAR PY 2015 VL 8 IS 3 BP 181 EP 189 DI 10.1158/1940-6207.CAPR-14-0172 PG 9 WC Oncology SC Oncology GA CE8FM UT WOS:000352077100001 PM 25586902 ER PT J AU Perez-Velazquez, J Quinones, B Hense, BA Kuttler, C AF Perez-Velazquez, Judith Quinones, Beatriz Hense, Burkhard A. Kuttler, Christina TI A mathematical model to investigate quorum sensing regulation and its heterogeneity in Pseudomonas syringae on leaves SO ECOLOGICAL COMPLEXITY LA English DT Article DE Pseudomonas syringae; Quorum sensing; Autoinducers; Population growth dynamics; Signal molecules; AHL; Stochastic process ID AERUGINOSA BIOFILMS; REGULATION SYSTEMS; GENE-EXPRESSION; SINGLE CELLS; LEAF SURFACE; BACTERIA; PHYLLOSPHERE; SIZE; AVAILABILITY; ENVIRONMENT AB Pseudomonas syringae is a plant-pathogen which, through the signalling system quorum sensing (QS), controls virulence. In this paper, we use the integral of a non-negative stochastic process to study the QS state of the bacterial colonies it forms when living on leaf surfaces. We investigate the extent to which factors such as water availability and diffusional losses of QS signalling molecules (autoinducers) would affect QS across colonies. Our results support that QS activation is indeed a good indicator of diffusional limitation, as QS is enhanced when diffusion of autoinducers signal decreases (either as a result of water availability or loss by diffusion). Using further experimental data, we explore heterogeneity of QS activation of this bacterium (colonies do not become homogeneously activated) when growing in this natural habitat. We extend our model to test a hypothesis regarding the initial QS potential of the cells. We are able to conclude that stochastic growth and uneven nutrient availability of the leaf surface may contribute only partially to the heterogeneity observed. We discuss the possible (evolutionary) explanations of this strategy. (C) 2014 Elsevier B.V. All rights reserved. C1 [Perez-Velazquez, Judith; Hense, Burkhard A.] German Res Ctr Enviromental Hlth, Helmholtz Zentrum Munchen, Inst Computat Biol, D-85764 Neuherberg, Germany. [Quinones, Beatriz] USDA, Western Reg Res Ctr, Produce Safety & Microbiol Unit, Berkeley, CA 94710 USA. [Kuttler, Christina] Tech Univ Munich, Ctr Math Sci, D-85747 Garching, Germany. RP Perez-Velazquez, J (reprint author), German Res Ctr Enviromental Hlth, Helmholtz Zentrum Munchen, Inst Computat Biol, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany. EM perez-velazquez@helmholtz-muenchen.de; beatriz.quinones@ars.usda.gov; burkhard.hense@helmholtz-muenchen.de; kuttler@ma.tum.de RI Perez-Velazquez, Judith/B-2460-2008 OI Perez-Velazquez, Judith/0000-0003-0969-075X NR 51 TC 5 Z9 5 U1 4 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1476-945X EI 1476-9840 J9 ECOL COMPLEX JI Ecol. Complex. PD MAR PY 2015 VL 21 SI SI BP 128 EP 141 DI 10.1016/j.ecocom.2014.12.003 PG 14 WC Ecology SC Environmental Sciences & Ecology GA CE6SA UT WOS:000351967200015 ER PT J AU Vandegehuchte, ML Sylvain, ZA Reichmann, LG De Tomasel, CM Nielsen, UN Wall, DH Sala, OE AF Vandegehuchte, Martijn L. Sylvain, Zachary A. Reichmann, Lara G. De Tomasel, Cecilia Milano Nielsen, Uffe N. Wall, Diana H. Sala, Osvaldo E. TI Responses of a desert nematode community to changes in water availability SO ECOSPHERE LA English DT Article DE drought; precipitation; resilience; soil ecology; soil moisture; trophic groups ID GRASSLAND ECOSYSTEMS; POPULATION-DYNAMICS; SIMULATED RAINFALL; FEEDING NEMATODES; NORTH-AMERICA; SOIL BIOTA; MOISTURE; TEMPERATURE; NITROGEN; MODEL AB The most recent climate models unequivocally predict a strong drying trend for the southwestern USA within the next century. Soil nematodes are a highly important component of desert ecosystem functioning, but rely on water films formovement. However, it is currently poorly understood how different trophic groups of nematodes respond to chronic presses as well as short-lived pulses of altered water availability, especially in arid systems where such changes are expected to have the greatest impact. The aim of this study was to assess the effects of both instantaneous and long-term variation in water availability on desert soil nematode trophic groups. We hypothesized that nematode abundance would respond positively to both short-and long-term increases in moisture. Based on the ecology of the different trophic groups we further made predictions about their relative rates of response. We increased or decreased precipitation from ambient levels in the Chihuahuan Desert for four consecutive years and sampled soil nematodes after two, three and four years. We tested the effects of altered precipitation treatments through time as well as gravimetric soil moisture at the time of sampling on the abundance of the different nematode trophic groups. In contrast to our hypotheses, the abundances of most nematode trophic groups were unaffected by the amount of precipitation, even after four years of altered precipitation. Plant-parasitic nematodes from low moisture soils were the only group that reacted positively to increased precipitation from the third year onwards. Trophic groups responded differently to soil moisture, with bacterivores decreasing with increasing moisture and omnivores showing a positive relationship that diminished over time. We show that in general, these desert nematodes were not limited by precipitation, and were highly resilient to decreases therein. However, when also considering the effects of soil moisture, some more complex patterns and differences among trophic groups emerged. We discuss potential mechanisms explaining these observations and contrast our findings with those from other ecosystems around the world. We conclude that deserts harbor nematode communities that seem more resilient to altered water availability than other ecosystems. C1 [Vandegehuchte, Martijn L.; Sylvain, Zachary A.; De Tomasel, Cecilia Milano; Nielsen, Uffe N.; Wall, Diana H.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Vandegehuchte, Martijn L.; Sylvain, Zachary A.; De Tomasel, Cecilia Milano; Nielsen, Uffe N.; Wall, Diana H.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Vandegehuchte, Martijn L.] Swiss Fed Inst Forest Snow & Landscape Res WSL, Res Unit Community Ecol, CH-8903 Birmensdorf, Switzerland. [Sylvain, Zachary A.] Atlantic Forestry Ctr, Canadian Forest Serv, Nat Resources Canada, Fredericton, NB E3B 5P7, Canada. [Reichmann, Lara G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Nielsen, Uffe N.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Nielsen, Uffe N.] Univ Western Sydney, Sch Sci & Hlth, Penrith, NSW 2751, Australia. [Sala, Osvaldo E.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Sala, Osvaldo E.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Sala, Osvaldo E.] New Mexico State Univ, Jornada Basin Long Term Ecol Res Program, Las Cruces, NM 88003 USA. RP Vandegehuchte, ML (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM martijn.vandegehuchte@wsl.ch RI Sylvain, Zachary/F-4828-2011 OI Sylvain, Zachary/0000-0003-3048-6067 FU National Science Foundation [DEB 09-17668, DEB 12-35828] FX We would like to thank the Jornada LTER, JERUSDA personnel and especially Kris Havstad and David Thatcher for their logistic support. This work was supported by National Science Foundation grants DEB 09-17668 and DEB 12-35828. NR 45 TC 2 Z9 2 U1 2 U2 31 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2015 VL 6 IS 3 AR 44 DI 10.1890/ES14-00319.1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA CF0AW UT WOS:000352205900017 ER PT J AU Jones, K Ramakrishnan, G Uchimiya, M Orlov, A AF Jones, Keith Ramakrishnan, Girish Uchimiya, Minori Orlov, Alexander TI New Applications of X-ray Tomography in Pyrolysis of Biomass: Biochar Imaging SO ENERGY & FUELS LA English DT Article ID BLACK CARBON BIOCHAR; CHEMICAL-COMPOSITION; MOLECULAR-STRUCTURE; MICRO-CT; TEMPERATURE; PLANT AB We report on the first ever use of nondestructive micrometer-scale synchrotron-computed microtomography (CMT) for biochar material characterization as a function of pyrolysis temperature. This innovative approach demonstrated an increase in micron-sized marcropore fraction of the cotton hull (CH) sample, resulting in up to 29% sample porosity. We have also found that initial porosity development occurred at low temperatures (below 350 degrees C) of pyrolysis, consistent with chemical composition of CH. This innovative technique can be highly complementary to traditional BET measurements, considering that Barrett-Joyner-Halenda (BJH) analysis of pore size distribution cannot detect these macropores. Such information can be of substantial relevance to environmental applications, given that water retention by biochars added to soils is controlled by macropore characteristic among the other factors. Complementing our data with SEM, EDX, and XRF characterization techniques allowed us to develop a better understanding of evolution of biochar properties during its production, such presence of metals and initial morphological features of biochar before pyrolysis. These results have significant implications for using biochar as a soil additive and for clarifying the mechanisms of biofuel production by pyrolysis. C1 [Jones, Keith; Ramakrishnan, Girish; Orlov, Alexander] SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11794 USA. [Jones, Keith] Brookhaven Natl Lab, Environm & Climate Sci Dept, Upton, NY 11973 USA. [Uchimiya, Minori] USDA, ARS Southern Reg Res Ctr, New Orleans, LA 70124 USA. RP Orlov, A (reprint author), SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11794 USA. FU U.S. Department of Energy [DE-AC02-98CH10886] FX Work was supported in part by the U.S. Department of Energy under contract No: DE-AC02-98CH10886. The U.S. DOE, Office of Science, and Office of Basic Energy Sciences supported use of National Synchrotron Light Source. NR 26 TC 1 Z9 1 U1 10 U2 57 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD MAR PY 2015 VL 29 IS 3 BP 1628 EP 1634 DI 10.1021/ef5027604 PG 7 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA CE2NV UT WOS:000351653200035 ER PT J AU Su, S Miska, KB Fetterer, RH Jenkins, MC Wong, EA AF Su, S. Miska, K. B. Fetterer, R. H. Jenkins, M. C. Wong, E. A. TI Expression of digestive enzymes and nutrient transporters in Eimeria-challenged broilers SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE Eimeria acervulina; Eimeria maxima; Eimeria tenella; Chickens; Nutrient transporters; Liver-expressed antimicrobial peptide 2 ID MESSENGER-RNA EXPRESSION; MAXIMA-INFECTED CHICKENS; AMINO-ACID TRANSPORTERS; ANTIMICROBIAL PEPTIDE; SMALL-INTESTINE; DEVELOPMENTAL REGULATION; CAMPYLOBACTER-JEJUNI; AMINOPEPTIDASE-N; ABUNDANCE; RESPONSES AB Avian coccidiosis is a disease caused by the intestinal protozoa Eimeria. The site of invasion and lesions in the intestine is species-specific, for example Eimeria acervulina affects the duodenum, Eimeria maxima the jejunum, and Eimeria tenella the ceca. Lesions in the intestinal mucosa cause reduced feed efficiency and body weight gain. The growth reduction may be due to changes in expression of digestive enzymes and nutrient transporters in the intestine. The objective of this study was to compare the expression of digestive enzymes, nutrient transporters and an antimicrobial peptide in broilers challenged with either Eimeria acervulina, Eimeria maxima or Eimeria tenella. The genes examined included digestive enzymes (APN and SI), peptide and amino acid transporters (PepT1, ASCT1, b(0,+)AT/rBAT, B(0)AT, CAT1, CAT2, EAAT3, LAT1, y(+)LAT1 and y(+)LAT2), sugar transporters (GLUT1, GLUT2, GLUT5 and SGLT1), zinc transporter (ZnT1) and an antimicrobial peptide (LEAP2). Duodenum, jejunum, ileum and ceca were collected 7 days post challenge. Eimeria acervulina challenge resulted in downregulation of various nutrient transporters or LEAP2 in the duodenum and ceca, but not the jejunum or ileum. Eimeria maxima challenge produced both downregulation and upregulation of nutrient transporters and LEAP2 in all three segments of the small intestine and ceca. Eimeria tenella challenge resulted in the downregulation and upregulation of nutrient transporters and LEAP2 in the jejunum, ileum and ceca, but not the duodenum. At the respective target tissue, Eimeria acervulina, Eimeria maxima and Eimeria tenella infection caused common downregulation of APN, b(0,+)AT, rBAT, EAAT3, SI, GLUT2, GLUT5, ZnT1 and LEAP2. The downregulation of nutrient transporters would result in a decrease in the efficiency of protein and polysaccharide digestion and uptake, which may partially explain the weight loss. The downregulation of nutrient transporters may also be a cellular response to reduced expression of the host defense protein LEAP2, which would diminish intracellular pools of nutrients and inhibit pathogen replication. (C) 2015 Elsevier Inc. All rights reserved. C1 [Su, S.; Wong, E. A.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. [Miska, K. B.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD USA. [Fetterer, R. H.; Jenkins, M. C.] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD USA. RP Wong, EA (reprint author), Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. EM ewong@vt.edu FU John Lee Pratt Animal Nutrition Program in the College of Agriculture and Life Sciences at Virginia Tech; USDA National Institute of Food and Agriculture multistate project [1002561] FX SS was supported by a fellowship from the John Lee Pratt Animal Nutrition Program in the College of Agriculture and Life Sciences at Virginia Tech. This work was supported in part by the USDA National Institute of Food and Agriculture multistate project 1002561. NR 29 TC 6 Z9 6 U1 6 U2 21 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 EI 1090-2449 J9 EXP PARASITOL JI Exp. Parasitol. PD MAR PY 2015 VL 150 BP 13 EP 21 DI 10.1016/j.exppara.2015.01.003 PG 9 WC Parasitology SC Parasitology GA CE8UZ UT WOS:000352120900003 PM 25617757 ER PT J AU Rieman, BE Smith, CL Naiman, RJ Ruggerone, GT Wood, CC Huntly, N Merrill, EN Alldredge, JR Bisson, PA Congleton, J Fausch, KD Levings, C Pearcy, W Scarnecchia, D Smouse, P AF Rieman, Bruce E. Smith, Courtland L. Naiman, Robert J. Ruggerone, Gregory T. Wood, Chris C. Huntly, Nancy Merrill, Erik N. Alldredge, J. Richard Bisson, Peter A. Congleton, James Fausch, Kurt D. Levings, Colin Pearcy, William Scarnecchia, Dennis Smouse, Peter TI A Comprehensive Approach for Habitat Restoration in the Columbia Basin SO FISHERIES LA English DT Article ID GENETIC STOCK IDENTIFICATION; CHINOOK SALMON; RIVER-BASIN; ADAPTIVE MANAGEMENT; PACIFIC SALMON; CLIMATE-CHANGE; UNITED-STATES; FOOD WEBS; CONSERVATION; ECOSYSTEMS C1 [Rieman, Bruce E.] US Forest Serv, Rocky Mt Res Stn, Seeley Lake, MT 59868 USA. [Smith, Courtland L.] Oregon State Univ, Sch Language Culture & Soc, Corvallis, OR 97331 USA. [Naiman, Robert J.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Naiman, Robert J.] Univ Western Australia, Ctr Excellence Nat Resource Management, Albany, WA, Australia. [Ruggerone, Gregory T.] Nat Resources Consultants, Seattle, WA USA. [Wood, Chris C.] Fisheries & Oceans Canada, Conservat Biol Sect, Nanaimo, BC, Canada. [Huntly, Nancy] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Merrill, Erik N.] Northwest Power & Conservat Council, Portland, OR USA. [Alldredge, J. Richard] Washington State Univ, Dept Stat, Pullman, WA 99164 USA. [Bisson, Peter A.] US Forest Serv, Forestry Sci Lab, Pacific NW Res Stn, Olympia, WA USA. [Congleton, James] Univ Idaho, Idaho Cooperat Fish & Wildlife Res Unit, Moscow, ID 83843 USA. [Fausch, Kurt D.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Levings, Colin] Fisheries & Oceans Canada, Ctr Aquaculture & Environm Res, W Vancouver, BC, Canada. [Pearcy, William] Oregon State Univ, Coll Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Scarnecchia, Dennis] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83843 USA. [Smouse, Peter] Rutgers State Univ, Ecol Evolut & Nat Resources, New Brunswick, NJ 08903 USA. EM brieman@blackfoot.net OI Huntly, Nancy/0000-0001-6051-6365 NR 114 TC 8 Z9 8 U1 5 U2 31 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0363-2415 EI 1548-8446 J9 FISHERIES JI Fisheries PD MAR PY 2015 VL 40 IS 3 BP 124 EP 135 PG 12 WC Fisheries SC Fisheries GA CE7MC UT WOS:000352023900011 ER PT J AU Porter, SD Valles, SM Wild, AL Dieckmann, R Plowes, NJR AF Porter, Sanford D. Valles, Steven M. Wild, Alexander L. Dieckmann, Roberta Plowes, Nicola J. R. TI Solenopsis invicta virus 3: Further host-specificity tests with native Solenopsis ants (Hymenoptera: Formicidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE Solenopsis invicta; biocontrol; host range; thief ant; native fire ant; SINV-3 ID FIRE ANTS AB A thorough understanding of host specificity is essential before pathogens can be used as biopesticides or self-sustaining biocontrol agents. In order to better define the host range of Solenopsis invicta virus 3 (SINV-3), we collected and exposed colonies of 2 native fire ants (Solenopsis aurea Wheeler, Solenopsis xyloni McCook) and 2 native thief ants (Solenopsis carolinensis Forel, Solenopsis molesta [Say]) (Hymenoptera: Formicidae) to the SINV-3 virus. Despite extreme exposure to the virus, active, replicating infections resulted only in colonies of the red imported fire ant (Solenopsis invicta Buren). These results, combined with a previous study of ants from 13 other genera, strongly support the proposition that the SINV-3 virus can be used safely as either a biopesticide or a self-sustaining biocontrol agent in parts of California, the Caribbean, and other regions of the world where this virus does yet not occur. C1 [Porter, Sanford D.; Valles, Steven M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Wild, Alexander L.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Dieckmann, Roberta] Coachella Valley Mosquito & Vector Control Dist, Indio, CA 92201 USA. [Plowes, Nicola J. R.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. RP Porter, SD (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM sanford.porter@ars.usda.gov NR 11 TC 4 Z9 4 U1 0 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 122 EP 125 PG 4 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500019 ER PT J AU Owens, D Nuessly, GS Gates, M AF Owens, David Nuessly, Gregg S. Gates, Michael TI Pachycrepoideus vindemmiae (Hymenoptera: Pteromalidae) as a potential natural enemy of maize-infesting Ulidiidae SO FLORIDA ENTOMOLOGIST LA English DT Article DE Pachycrepoideus vindemmia; Euxesta eluta; Ulidiidae; insecticides ID FRUIT-FLY DIPTERA; CORN; PARASITOIDS; PESTS AB Euxesta annonae Fabricius, E. eluta Loew, E. stigmatias Loew, and Chaetopsis massyla Walker (Diptera: Ulidiidae) are primary sweet corn pests in Florida. Few natural enemies of these flies are known. The pupal parasitoid Pachycrepoideus vindemmiae Rondani (Hymenoptera: Pteromalidae) was discovered in a laboratory colony of E. eluta and E. stigmatias, and its potential as a biological control agent was studied. Development times in fresh, chilled, and frozen E. eluta pupae were recorded. Fly larvae were allowed to dig into soil to pupate, and pupae covered by 2.5 cm of soil were presented to wasps to determine if Pachycrepoideus vindemmiae could locate them. Finally, to evaluate susceptibility to insecticides commercially used in ear-stage sweet corn, adult parasitoids were caged on maize leaves treated with chlorpyrifos, methomyl, or zeta cypermethrin for 24 h. Pachycrepoideus vindemmiae completed development in normal, chilled, and frozen fly pupae in 15-17 days. None of the fly pupae covered by soil were parasitized. Chlorpyrifos and methomyl residues killed >95% of Pachycrepoideus vindemmiae within 24 h. Zeta cypermethrin was slower acting, but resulted in 50% mortality after 24 h. Therefore, Pachycrepoideus vindemmiae does not appear to be well suited as an effective biological control agent of maize-infesting Ulidiidae in sweet corn fields. This is the first known account of this cosmopolitan parasitoid attacking maize-infesting ulidiids. C1 [Owens, David; Nuessly, Gregg S.] Univ Florida, Dept Entomol & Nematol, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [Gates, Michael] USDA ARS, Smithsonian Inst, Natl Museum Nat Hist, Systemat Entomol Lab, Washington, DC 20013 USA. RP Owens, D (reprint author), Univ Florida, Dept Entomol & Nematol, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. EM owensd119@ufl.edu NR 24 TC 2 Z9 2 U1 2 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 276 EP 279 PG 4 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500044 ER PT J AU Vega, FE Simpkins, A Bauchan, G Valdez-Carrasco, JM Castillo, A Infante, F AF Vega, Fernando E. Simpkins, Ann Bauchan, Gary Valdez-Carrasco, Jorge M. Castillo, Alfredo Infante, Francisco TI A mysterious wing spine in male coffee berry borers (Coleoptera: Curculionidae: Scolytinae) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Hypothenemus hampei; broca del cafe; stridulation; acoustics AB A spine was observed in the hind wings of male coffee berry borers, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae), although it was not present in all males. Females do not exhibit the spine. The function of the spine remains unknown although one possibility is that it might have had a stridulatory role in acoustic communication. C1 [Vega, Fernando E.; Simpkins, Ann] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Bauchan, Gary] USDA ARS, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA. [Valdez-Carrasco, Jorge M.] Colegio Postgrad, Texcoco, Edo De Mexico, Mexico. [Castillo, Alfredo; Infante, Francisco] El Colegio Frontera ECOSUR, Tapachula 30700, Chiapas, Mexico. RP Vega, FE (reprint author), USDA ARS, Sustainable Perennial Crops Lab, Bldg 001, Beltsville, MD 20705 USA. EM Fernando.Vega@ars.usda.gov RI Alfredo, Castillo/B-5491-2016; OI Alfredo, Castillo/0000-0003-1452-0026; Infante, Francisco/0000-0002-7419-7606; Vega, Fernando E./0000-0001-8103-5640 NR 4 TC 1 Z9 1 U1 1 U2 12 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 352 EP 353 PG 2 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500054 ER PT J AU Amalin, DM Averion, L Bihis, D Legaspi, JC David, EF AF Amalin, Divina M. Averion, Lani Bihis, Dennis Legaspi, Jesusa C. David, Edward F. TI Effectiveness of kaolin clay particle film in managing Helopeltis collaris (Hemiptera: Miridae), a major pest of cacao in The Philippines SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE cacao mirid, capsid bug; particle film; repellent; soybean oil as sticker-spreader AB The use of particle film technology has not been documented previously to protect cacao (Theobroma cacao L.; Malvales: Malvaceae) against insect feeding. Laboratory assessment of the use of local kaolin against the cacao mirid bug, Helopeltis collaris Stal (Hemiptera: Miridae), revealed a highly positive and statistically significant repellence of this insect pest on kaolin-treated cacao pods. This result warrants follow up field studies to evaluate further the potential of kaolin clay to control cacao pests in the Philippines. C1 [Amalin, Divina M.] De La Salle Univ, Coll Sci, Dept Biol, Manila 1004, Philippines. [Averion, Lani; Bihis, Dennis] Quezon Agr Expt Stn, Quezon City, Philippines. [Legaspi, Jesusa C.] USDA ARS, Ctr Med Agr & Vet Entomol, Tallahassee, FL 32308 USA. [Amalin, Divina M.; David, Edward F.] Cocoa Fdn Philippines, Quezon City, Philippines. RP Amalin, DM (reprint author), De La Salle Univ, Coll Sci, Dept Biol, 2401 Taft Ave, Manila 1004, Philippines. EM divina.amalin@dlsu.edu.ph NR 13 TC 0 Z9 0 U1 2 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 354 EP 355 PG 2 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500055 ER PT J AU Ammar, ED Hall, DG Shatters, RG AF Ammar, El-Desouky Hall, David G. Shatters, Robert G., Jr. TI Ultrastructure and development of the new stylets inside pre-molting first instar nymphs of the Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE bacteriome; citrus greening disease; filter chamber; huanglongbing; mandibular stylets; maxillary stylets AB The ultrastructure and development of new stylets was studied in pre-molting first instar nymph of Diaphorina citri Kuwayama (Hemiptera: Liviidae). Two oval-shaped masses of cuboidal hypodermal cells, located in the cephalic region, had long extensions that ended with developing pairs of mandibular and maxillary stylets, apparently coiled around these masses. A new structure, probably composed of softer cuticle, was found on the ventral side of each developing stylet suggesting that this structure may work as a mold during formation of the new stylets. Other organs of 1st instar nymphs, including the filter chamber and bacteriome, are also ultrastructurally described. C1 [Ammar, El-Desouky; Hall, David G.; Shatters, Robert G., Jr.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Ammar, ED (reprint author), USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM desoukyammar@gmail.com NR 8 TC 0 Z9 0 U1 3 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 373 EP 376 PG 4 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500062 ER PT J AU Diaz, R Romero, S Roda, A Mannion, C Overholt, WA AF Diaz, R. Romero, S. Roda, A. Mannion, C. Overholt, W. A. TI Diversity of arthropods associated with Mikania spp. and Chromolaena odorata (Asterales: Asteraceae: Eupatorieae) in Florida SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Chromolaena odorata; herbivore diversity; Mikania cordifolia; Mikania scandens ID BIOLOGICAL-CONTROL; HOST PLANTS; LEPIDOPTERA; ACARI; PSEUDOCOCCIDAE; TORTRICIDAE; CALIFORNIA; HOMOPTERA; HEMIPTERA; INVASIONS AB Field surveys revealed that in south Florida the recently established Mikania micrantha Kunth (Asterales: Asteraceae: Eupatorieae) had a lower diversity of monophagous insect herbivores compared to the native Mikania spp. or Chromolaena odorata (L.) R. M. King & H. Rob. (Asterales: Asteraceae: Eupatorieae). In addition, Mikania micrantha served as a host for pests of agricultural and ornamental crops in Florida. C1 [Diaz, R.; Overholt, W. A.] Univ Florida, Indian River Res & Educ Ctr, Biol Control Res & Containment Lab, Ft Pierce, FL 34981 USA. [Romero, S.; Mannion, C.] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. [Roda, A.] US Anim & Plant Hlth Inspect Serv, USDA, Miami, FL USA. RP Overholt, WA (reprint author), Univ Florida, Indian River Res & Educ Ctr, Biol Control Res & Containment Lab, Ft Pierce, FL 34981 USA. EM billover@ufl.edu NR 53 TC 1 Z9 1 U1 2 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 389 EP 393 PG 5 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500067 ER PT J AU Harris-Shultz, K Ni, XZ Wang, HL Knoll, JE Anderson, WF AF Harris-Shultz, Karen Ni, Xinzhi Wang, Hongliang Knoll, Joseph E. Anderson, William F. TI Use of benzimidazole agar plates to assess fall armyworm (Lepidoptera: Noctuidae) feeding on excised maize and sorghum leaves SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Spodoptera frugiperda; Zea mays; Sorghum bicolor; whorl tissue; bioassay ID SOUTHWESTERN CORN-BORER; SPODOPTERA-FRUGIPERDA SMITH,J.E.; INSECTICIDE RESISTANCE; POPULATIONS; LINES; WEED AB The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is an economically significant pest of sorghum and maize. Laboratory bioassays are often conducted for convenience and for fall armyworm confinement but as the number of entries and replications increase, the replenishing of fresh tissue daily and the cleaning of insect diet cups or petri dishes becomes laborious. The current study was conducted to determine if agar plates, used to retain leaf moisture, supplemented with benzimidazole, a fungicide that delays leaf senescence and retards fungicide growth, can be used to assess fall armyworm feeding on fresh maize and sorghum leaf tissue with minimal labor. We conducted 2 trials consisting of 3 cultivars of maize with known resistance or susceptibility to fall armyworm feeding, and 4 cultivars of sorghum that are parents of existing mapping populations. The top 2 (whorl) leaves were removed from 36-37-day old plants, 7.6 cm long pieces of leaf were excised, and each piece was placed on a benzimidazole agar plate. One larva per plate was placed on the leaf tissue; the plate was sealed with Parafilm and placed in an incubator for 7 days. Average larval weights for Trial 1 were significantly different than Trial 2 after 7 days, and thus each trial was analyzed separately. For both trials, the larvae that were fed susceptible maize line 'AB24E' had weights that were significantly greater than larvae fed resistant maize lines 'Mp708' and 'FAW1430'. No significant differences in weight were observed for fall armyworm larvae fed on the 4 sorghum lines except for those larvae fed 'Collier' in Trial 2, which had weights significantly greater than larvae fed the other 3 sorghum cultivars (Entry 22, 'Honey Drip', 'AN109'). Thus, the benzimidazole agar plate method is an easy and effective method for assessing fall armyworm feeding on maize and sorghum, and thus can be used to identify maize and sorghum germplasm lines with resistance to fall armyworm. C1 [Harris-Shultz, Karen; Ni, Xinzhi; Wang, Hongliang; Knoll, Joseph E.; Anderson, William F.] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. RP Harris-Shultz, K (reprint author), USDA ARS, Crop Genet & Breeding Res Unit, 115 Coastal Way, Tifton, GA 31793 USA. EM Karen.Harris@ars.usda.gov NR 22 TC 0 Z9 0 U1 1 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2015 VL 98 IS 1 BP 394 EP 397 PG 4 WC Entomology SC Entomology GA CE8JD UT WOS:000352087500068 ER PT J AU Baez-Gonzalez, AD Kiniry, JR Ramirez, JSP Garcia, GM Gonzalez, JLR Ceja, ESO AF Delia Baez-Gonzalez, Alma Kiniry, James R. Padilla Ramirez, Jose Saul Medina Garcia, Guillemo Ramos Gonzalez, Jose Luis Osuna Ceja, Esteban Salvador TI PARAMETERIZATION OF ALMANAC CROP SIMULATION MODEL FOR NON-IRRIGATED DRY BEAN IN SEMI-ARID TEMPERATE AREAS IN MEXICO SO INTERCIENCIA LA English DT Article DE Dry Bean; Harvest Index; Leaf Area Index; Parameterization; Potential Heat Units; Simulation Models ID SORGHUM; MAIZE; TEXAS; GROWTH; SORKAM; YIELD AB Dry bean simulation models can be used to make management decisions when properly parameterized. This study aimed to parameterize the ALMANAC (Agricultural Land Management Alternatives with Numerical Assessment Criteria) crop simulation model for dry bean in the semi-arid temperate areas of Mexico. The parameterization process was based on data from two important non-irrigated dry bean fields in Mexico. The parameters were potential heat units (PHU), leaf area index (LAI) and harvest index (HI) for both adapted improved cultivars and native cultivars. Model performance with the parameters was evaluated by comparing simulated and measured yields. The model described much of the variability in measured yields; it had a root mean square error (RMSE) of 0.26 Mg.ha(-1). The mean squared deviation (MSD) was 0.11, and the values of its three components were 0.01 for squared bias (SB), 0.10 for lack of correlation weighted by standard deviation (LCS) and 0.001 for squared difference between standard deviations (SDSD). The derived crop parameters for native cultivars (1000 PHU, 0.3 HI and 0.6 LAI) and for improved cultivars (850-900 PHU, 0.46-0.50 HI and 0.7-1.5 LAI) have potential use for simulating dry bean in semi-arid temperate areas in Mexico. C1 [Delia Baez-Gonzalez, Alma] Univ Reading, Agr Syst Anal & Modelling, Reading RG6 2AH, Berks, England. [Delia Baez-Gonzalez, Alma] Inst Nacl Invest Forest Agr & Pecuarias, Tam, Mexico. [Kiniry, James R.] Texas A&M Univ, Crop Physiol, College Stn, TX 77843 USA. [Kiniry, James R.] USDA ARS, Grassland Soil & Water Res Lab, Washington, DC 20250 USA. [Padilla Ramirez, Jose Saul] New Mexico State Univ, Agronomy, Las Cruces, NM 88003 USA. [Medina Garcia, Guillemo] Univ Zacatecas, Livestock Prod, Zacatecas, Mexico. [Medina Garcia, Guillemo] INIFAP Calera, Zacatecas, Mexico. [Ramos Gonzalez, Jose Luis] Univ A de Aguascalientes, Agronomy, Aguascalientes, Mexico. [Osuna Ceja, Esteban Salvador] Colegio Posgrad, Hydroscience, Montecillos, Mexico. RP Baez-Gonzalez, AD (reprint author), Univ Reading, Agr Syst Anal & Modelling, Reading RG6 2AH, Berks, England. EM baez.alma@inifap.gob.mx NR 34 TC 0 Z9 0 U1 2 U2 2 PU INTERCIENCIA PI CARACAS PA APARTADO 51842, CARACAS 1050A, VENEZUELA SN 0378-1844 J9 INTERCIENCIA JI Interciencia PD MAR PY 2015 VL 40 IS 3 BP 185 EP 189 PG 5 WC Ecology SC Environmental Sciences & Ecology GA CE5QZ UT WOS:000351892000006 ER PT J AU Sundaresan, S Philosoph-Hadas, S Riov, J Belausov, E Kochanek, B Tucker, ML Meir, S AF Sundaresan, Srivignesh Philosoph-Hadas, Sonia Riov, Joseph Belausov, Eduard Kochanek, Betina Tucker, Mark L. Meir, Shimon TI Abscission of flowers and floral organs is closely associated with alkalization of the cytosol in abscission zone cells SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Abscission zone; alkalization; Arabidopsis mutants; cytosol; ethylene; flower organs; pH regulation; tomato (Solanum lycopersicum); wild rocket (Diplotaxis tenuifolia) ID DEFICIENT-IN-ABSCISSION; INFLORESCENCE-DEFICIENT; PH REGULATION; ARABIDOPSIS-THALIANA; INTRACELLULAR PH; SEPARATION PROCESSES; AMMONIA SECRETION; AUXIN DEPLETION; APOPLASTIC PH; TOMATO FRUIT AB In vivo changes in the cytosolic pH of abscission zone (AZ) cells were visualized using confocal microscopic detection of the fluorescent pH-sensitive and intracellularly trapped dye, 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF), driven by its acetoxymethyl ester. A specific and gradual increase in the cytosolic pH of AZ cells was observed during natural abscission of flower organs in Arabidopsis thaliana and wild rocket (Diplotaxis tenuifolia), and during flower pedicel abscission induced by flower removal in tomato (Solanum lycopersicum Mill). The alkalization pattern in the first two species paralleled the acceleration or inhibition of flower organ abscission induced by ethylene or its inhibitor 1-methylcyclopropene (1-MCP), respectively. Similarly, 1-MCP pre-treatment of tomato inflorescence explants abolished the pH increase in AZ cells and pedicel abscission induced by flower removal. Examination of the pH changes in the AZ cells of Arabidopsis mutants defective in both ethylene-induced (ctr1, ein2, eto4) and ethylene-independent (ida, nev7, dab5) abscission pathways confirmed these results. The data indicate that the pH changes in the AZ cells are part of both the ethylene-sensitive and -insensitive abscission pathways, and occur concomitantly with the execution of organ abscission. pH can affect enzymatic activities and/or act as a signal for gene expression. Changes in pH during abscission could occur via regulation of transporters in AZ cells, which might affect cytosolic pH. Indeed, four genes associated with pH regulation, vacuolar H+-ATPase, putative high-affinity nitrate transporter, and two GTP-binding proteins, were specifically up-regulated in tomato flower AZ following abscission induction, and 1-MCP reduced or abolished the increased expression. C1 [Sundaresan, Srivignesh; Philosoph-Hadas, Sonia; Kochanek, Betina; Meir, Shimon] Volcani Ctr, ARO, Dept Postharvest Sci Fresh Produce, IL-5025001 Bet Dagan, Israel. [Sundaresan, Srivignesh; Riov, Joseph] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, IL-7610001 Rehovot, Israel. [Belausov, Eduard] Volcani Ctr, ARO, Dept Ornamental Hort, IL-5025001 Bet Dagan, Israel. [Tucker, Mark L.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Meir, S (reprint author), Volcani Ctr, ARO, Dept Postharvest Sci Fresh Produce, IL-5025001 Bet Dagan, Israel. EM shimonm@volcani.agri.gov.il FU Indian Council of Agricultural Research; United States-Israel Binational Agricultural Research and Development Fund (BARD) [US-4571-12C]; Israeli Ministry of Agriculture [203-0898-10] FX Contribution No. 697/14 from the ARO, The Volcani Center, Bet Dagan, Israel. We would like to thank Dr Sara E. Patterson (University of Wisconsin-Madison, USA), for generously providing the Arabidopsis mutant lines. SS would like to thank the Indian Council of Agricultural Research for providing him with an International Fellowship (ICAR-IF), as partial support of his PhD studies. This work was supported by the United States-Israel Binational Agricultural Research and Development Fund (BARD) [grant no. US-4571-12C to SM, MLT, and SP-H], and the Chief Scientist of the Israeli Ministry of Agriculture Fund [grant no. 203-0898-10 to SM and SP-H]. NR 67 TC 3 Z9 3 U1 6 U2 18 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2015 VL 66 IS 5 BP 1355 EP 1368 DI 10.1093/jxb/eru483 PG 14 WC Plant Sciences SC Plant Sciences GA CE2RJ UT WOS:000351663300018 PM 25504336 ER PT J AU Saminathan, T Nimmakayala, P Manohar, S Malkaram, S Almeida, A Cantrell, R Tomason, Y Abburi, L Rahman, MA Vajja, VG Khachane, A Kumar, B Rajasimha, HK Levi, A Wehner, T Reddy, UK AF Saminathan, Thangasamy Nimmakayala, Padma Manohar, Sumanth Malkaram, Sridhar Almeida, Aldo Cantrell, Robert Tomason, Yan Abburi, Lavanya Rahman, Mohammad A. Vajja, Venkata G. Khachane, Amit Kumar, Brajendra Rajasimha, Harsha K. Levi, Amnon Wehner, Todd Reddy, Umesh K. TI Differential gene expression and alternative splicing between diploid and tetraploid watermelon SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE genome duplication; gene expression; RACE-PCR; RNA-seq; tetraploid; alternative splicing; watermelon (Citrullus lanatus) ID CITRULLUS-LANATUS THUNB.; MADS-BOX GENES; RNA-SEQ; ARABIDOPSIS ALLOTETRAPLOIDS; DUPLICATED GENES; GENOME DUPLICATIONS; PLANT POLYPLOIDS; MESSENGER-RNA; EVOLUTION; TRANSCRIPTS AB The exploitation of synthetic polyploids for producing seedless fruits is well known in watermelon. Tetraploid progenitors of triploid watermelon plants, compared with their diploid counterparts, exhibit wide phenotypic differences. Although many factors modulate alternative splicing (AS) in plants, the effects of autopolyploidization on AS are still unknown. In this study, we used tissues of leaf, stem, and fruit of diploid and tetraploid sweet watermelon to understand changes in gene expression and the occurrence of AS. RNA-sequencing analysis was performed along with reverse transcription quantitative PCR and rapid amplification of cDNA ends (RACE)-PCR to demonstrate changes in expression and splicing. All vegetative tissues except fruit showed an increased level of AS in the tetraploid watermelon throughout the growth period. The ploidy levels of diploids and the tetraploid were confirmed using a ploidy analyser. We identified 5362 and 1288 genes that were up- and downregulated, respectively, in tetraploid as compared with diploid plants. We further confirmed that 22 genes underwent AS events across tissues, indicating possibilities of generating different protein isoforms with altered functions of important transcription factors and transporters. Arginine biosynthesis, chlorophyllide synthesis, GDP mannose biosynthesis, trehalose biosynthesis, and starch and sucrose degradation pathways were upregulated in autotetraploids. Phloem protein 2, chloroplastic PGR5-like protein, zinc-finger protein, fructokinase-like 2, MYB transcription factor, and nodulin MtN21 showed AS in fruit tissues. These results should help in developing high-quality seedless watermelon and provide additional transcriptomic information related to other cucurbits. C1 [Saminathan, Thangasamy; Nimmakayala, Padma; Manohar, Sumanth; Malkaram, Sridhar; Almeida, Aldo; Cantrell, Robert; Tomason, Yan; Abburi, Lavanya; Rahman, Mohammad A.; Vajja, Venkata G.; Reddy, Umesh K.] West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. [Khachane, Amit; Kumar, Brajendra; Rajasimha, Harsha K.] Genome Int Corp, Madison, WI 53717 USA. [Levi, Amnon] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Wehner, Todd] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. RP Reddy, UK (reprint author), West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. EM ureddy@wvstateu.edu FU USDA-NIFA [2013-38821-21453]; NSF-EPSCOR [1003907]; Gus R. Douglass Institute; NIH [P20RR016477] FX This project was supported by the USDA-NIFA (no. 2013-38821-21453), NSF-EPSCOR (no. 1003907), Gus R. Douglass Institute, and NIH (no. P20RR016477). The authors are grateful to R. Jarret, PGRCU, USDA-ARS (Griffin, GA, USA) for providing the seeds for the germplasm accessions. NR 74 TC 7 Z9 7 U1 7 U2 64 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2015 VL 66 IS 5 BP 1369 EP 1385 DI 10.1093/jxb/eru486 PG 17 WC Plant Sciences SC Plant Sciences GA CE2RJ UT WOS:000351663300019 PM 25520388 ER PT J AU Beck, BH Barnett, LM Farmer, BD Peatman, E Carter, D AF Beck, B. H. Barnett, L. M. Farmer, B. D. Peatman, E. Carter, D. TI Kaolinitic clay protects against Flavobacterium columnare infection in channel catfish Ictalurus punctatus (Rafinesque) SO JOURNAL OF FISH DISEASES LA English DT Article DE catfish; columnaris disease; disease prevention; Flavobacterium columnare; kaolin ID POTASSIUM-PERMANGANATE; FLEXIBACTER-COLUMNARIS; SALMO-GAIRDNERI; COPPER-SULFATE; AQUACULTURE; DISEASE; FISH; ANTIBIOTICS; ENVIRONMENT; ADSORPTION AB Columnaris disease, caused by the bacterial pathogen Flavobacterium columnare, continues to be a major problem worldwide in both wild and cultured freshwater finfish. Despite the far-reaching negative impacts of columnaris disease, safe and efficacious preventatives and curatives for this disease remain limited. In this study, we evaluated the potential of kaolin (Al2Si2O5(OH)(4)), a type of clay, for the prevention of columnaris disease. Channel catfish, Ictalurus punctatus (Rafinesque), fingerlings were experimentally challenged with Flavobacterium columnare in untreated water or with water containing kaolin (1 g L-1). Over the 7-day course of study, kaolin treatment led to significantly (P < 0.001) improved survival (96%) as compared to untreated fish (78% survival). Histological examination of the gills revealed that kaolin-treated fish had substantially less gill damage than untreated controls. Quantitative PCR analysis of gill tissue revealed that kaolin significantly reduced F. columnare adhesion (measured at 1 h post-challenge) and colonization (24 h post-challenge). Incubation of kaolin with F. columnare in vitro demonstrated that kaolin reduced the number of F. columnare cells in culture supernatants, presumably through the formation of physical complexes through adsorption. In summary, kaolin can improve survival, reduce gill pathologies and reduce bacterial attachment to key tissues associated with columnaris disease in channel catfish by binding to F. columnare. C1 [Beck, B. H.; Barnett, L. M.; Farmer, B. D.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Peatman, E.] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. [Carter, D.] KaMin LLC, Macon, GA USA. RP Beck, BH (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM benjamin.beck@ars.usda.gov NR 33 TC 3 Z9 3 U1 2 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD MAR PY 2015 VL 38 IS 3 BP 241 EP 248 DI 10.1111/jfd.12229 PG 8 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA CE9EG UT WOS:000352146000001 PM 24499204 ER PT J AU Barba, M Stewart, AJ Passler, T Wooldridge, AA van Santen, E Chamorro, MF Cattley, RC Hathcock, T Hogsette, JA Hu, XP AF Barba, M. Stewart, A. J. Passler, T. Wooldridge, A. A. van Santen, E. Chamorro, M. F. Cattley, R. C. Hathcock, T. Hogsette, J. A. Hu, X. P. TI Experimental Transmission of Corynebacterium pseudotuberculosis Biovar equi in Horses by House Flies SO JOURNAL OF VETERINARY INTERNAL MEDICINE LA English DT Article DE Equine; Musca domestica; Pigeon fever; Vector ID INFECTION AB BackgroundThe route of Corynebacterium pseudotuberculosis infection in horses remains undetermined, but transmission by insects is suspected. ObjectivesTo investigate house flies (Musca domestica L.) as vectors of C.pseudotuberculosis transmission in horses. AnimalsEight healthy, adult ponies. MethodsRandomized, controlled, blinded prospective study. Ten wounds were created in the pectoral region where cages for flies were attached. Three ponies were directly inoculated with C.pseudotuberculosis. Four ponies were exposed for 24hours to 20hours C.pseudotuberculosis-inoculated flies. One negative control pony was exposed to noninoculated flies. Ponies were examined daily for swelling, heat, pain, and drainage at the inoculation site. Blood was collected weekly for CBC and biochemical analysis, and twice weekly for synergistic hemolysis inhibition titers. ResultsClinical signs of local infection and positive cultures were observed in 7/7 exposed ponies and were absent in the negative control. In exposed ponies, peak serologic titers (1:512 to 1:2,048) were obtained between days 17 and 21. Seroconversion was not observed in the negative control. Neutrophil counts were higher in the positive and fly-exposed groups than in the negative control (P=.002 and P=.005) on day 3 postinoculation. Serum amyloid A concentrations were higher in the positive control than in the negative control and fly-exposed ponies on days 3 (P<.0001) and 7 (P=.0004 and P=.0001). No differences were detected for other biochemical variables. Conclusions and Clinical ImportanceHouse flies can serve as mechanical vectors of C.pseudotuberculosis and can transmit the bacterium to ponies. C1 [Barba, M.; Stewart, A. J.; Passler, T.; Wooldridge, A. A.; Chamorro, M. F.] Auburn Univ, Coll Vet Med, Dept Clin Sci, Auburn, AL 36849 USA. [van Santen, E.] Auburn Univ, Coll Vet Med, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. [Cattley, R. C.; Hathcock, T.] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA. [Hogsette, J. A.] USDA ARS CMAVE, Gainesville, FL USA. [Hu, X. P.] Auburn Univ, Dept Entomol & Plant Pathol, Coll Agr, Auburn, AL 36849 USA. RP Stewart, AJ (reprint author), Auburn Univ, Large Anim Teaching Hosp, Equine Internal Med, 1500 Wire Rd, Auburn, AL 36849 USA. EM stewaaj@auburn.edu RI Stewart, Allison/E-4355-2016; Barba Recreo, Marta/I-1888-2016 OI Stewart, Allison/0000-0002-2464-3954; Barba Recreo, Marta/0000-0002-3677-7239 FU Boehringer Ingelheim Vetmedica, Inc; Auburn University College of Veterinary Medicine Animal Health and Disease Research Funds FX This research was supported by the Boehringer Ingelheim Vetmedica, Inc, Advancement of Equine Research Award and Auburn University College of Veterinary Medicine Animal Health and Disease Research Funds. We acknowledge Auburn University, Sugg Laboratory personnel for use of BL-2 research facilities; Qiao Zhong; students Casey Randle, Amy Ramos, Jillian Costello, Blake Dewitt and Kacy Riley for technical assistance; Cole Baker for necropsy assistance; and Dr. Tiffany Durham from Salinas, CA for providing the field strain of Corynebacterium pseudotuberculosis. NR 23 TC 4 Z9 4 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0891-6640 EI 1939-1676 J9 J VET INTERN MED JI J. Vet. Intern. Med. PD MAR-APR PY 2015 VL 29 IS 2 BP 636 EP 643 DI 10.1111/jvim.12545 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CE5BW UT WOS:000351845900022 PM 25818218 ER PT J AU Olarte, RA Horn, BW Singh, R Carbone, I AF Olarte, Rodrigo A. Horn, Bruce W. Singh, Rakhi Carbone, Ignazio TI Sexual recombination in Aspergillus tubingensis SO MYCOLOGIA LA English DT Article DE Aspergillus niger; mating type; multilocus sequence typing; sexual reproduction ID AFLATOXIN GENE-CLUSTER; CITRIC-ACID PRODUCTION; MATING-TYPE GENES; FLAVUS; REPRODUCTION; PARASITICUS; NIGER; FERMENTATION; MUTAGENESIS; IMPROVEMENT AB Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and beta-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production. C1 [Olarte, Rodrigo A.; Singh, Rakhi; Carbone, Ignazio] N Carolina State Univ, Dept Plant Pathol, Ctr Integrated Fungal Res, Raleigh, NC 27695 USA. [Horn, Bruce W.] ARS, USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA. RP Carbone, I (reprint author), N Carolina State Univ, Dept Plant Pathol, Ctr Integrated Fungal Res, Raleigh, NC 27695 USA. EM icarbon@gncsu.edu FU Agriculture and Food Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture (NIFA) [2013-68004-20359]; USDA-NIFA Pre-doctoral fellowship [2012-67011-19689] FX We thank Travis Walk for technical assistance. This work was supported by the Agriculture and Food Research Initiative Competitive Grants Program grant No. 2013-68004-20359 from the USDA National Institute of Food and Agriculture (NIFA). RAO was supported by a USDA-NIFA Pre-doctoral fellowship, grant No. 2012-67011-19689. NR 36 TC 0 Z9 0 U1 3 U2 10 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2015 VL 107 IS 2 BP 307 EP 312 DI 10.3852/14-233 PG 6 WC Mycology SC Mycology GA CE5RF UT WOS:000351892600005 PM 25572097 ER PT J AU Richard, F Bellanger, JM Clowez, P Hansen, K O'Donnell, K Urban, A Sauve, M Courtecuisse, R Moreau, PA AF Richard, Franck Bellanger, Jean-Michel Clowez, Philippe Hansen, Karen O'Donnell, Kerry Urban, Alexander Sauve, Mathieu Courtecuisse, Regis Moreau, Pierre-Arthur TI True morels (Morchella, Pezizales) of Europe and North America: evolutionary relationships inferred from multilocus data and a unified taxonomy SO MYCOLOGIA LA English DT Article DE Ascomycota; Morchellaceae; nomenclature; Pezizomycetes; taxonomy ID INTERNAL TRANSCRIBED SPACER; PHYLOGENETIC ANALYSIS; RIBOSOMAL DNA; ASCOMYCOTA; DIVERSITY; CRASSIPES; SEQUENCES; REVEALS; TURKEY; FUNGI AB Applying early names, with or without original material, to genealogical species is challenging. For morels this task is especially difficult because of high morphological stasis and high plasticity of apothecium color and shape. Here we propose a nomenclatural revision of true morels (Morchella, Pezizales) from Europe and North America, based on molecular phylogenetic analyses of portions of the genes for RNA polymerase II largest subunit (RPB1) and second largest subunit (RPB2), translation elongation factor-1 alpha (TEF1), the nuc rDNA region encompassing the internal transcribed spacers 1 and 2, along with the 5.8S rDNA (ITS), and partial nuc 28S rDNA D1-D2 domains (28S). The 107 newly sequenced collections were from both continents, including 48 types, together with previously published sequences. Names are applied to 30 of the 65 currently recognized genealogical species. Results of the present study revealed that the number of Morchella species in Europe (n = 21) is nearly identical to that in North America (n = 22). Only seven species were found on both continents, consistent with previous reports of high continental endemism within the genus. Presently it is not possible to tell whether the transoceanic disjunctions were due to human activities, migration across a Bering land bridge or long-distance dispersal. In an effort to stabilize the taxonomy, due in part to the recent publication of synonyms for 11 of the species, accepted names are presented together with their corresponding later synonyms. A new subclade that includes holotypes of M. castanea and M. brunneorosea is identified in sect. Morchella (Esculenta Clade). Lectotypes for Morchella deliciosa, M. eximia and M. tridentina are designated here, as well as epitypes for M. dunalii, M. eximia, M. purpurascens and M. vulgaris. Morchella conica was determined to be illegitimate, and further research is required to determine the identity of M. elata and M. inamoena. C1 [Richard, Franck; Bellanger, Jean-Michel] INSERM, UMR CEFE 5175, F-34293 Montpellier, France. [Hansen, Karen] Swedish Museum Nat Hist, Dept Bot, SE-10405 Stockholm, Sweden. [O'Donnell, Kerry] ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Urban, Alexander] Univ Vienna, Fac Life Sci, Dept Bot & Biodivers Res, Div Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Sauve, Mathieu] Univ Montpellier, UMR CEFE 5175, F-34293 Montpellier, France. [Courtecuisse, Regis; Moreau, Pierre-Arthur] Univ Lille Nord France, Dept Sci Vegetales & Fong, Fac Sci Pharmaceut & Biol, F-59000 Lille, France. [Courtecuisse, Regis; Moreau, Pierre-Arthur] UFR Pharm, F-59000 Lille, France. RP Richard, F (reprint author), INSERM, UMR CEFE 5175, Campus CNRS,1919 Route Mende, F-34293 Montpellier, France. EM franck.richard@cefe.cnrs.fr FU grant "Diversite des champignons mycorhiziens des plantes" (DivMyc, to Marc-Andre Selosse) from the network Bibliotheque Du Vivant (BDV) - CNRS; Museum National d'Histoire Nature lle de Paris; INRA; CEA (Centre National de Sequencage); BDV program FX We are especially grateful for the help of the curators of the herbaria who kindly allowed us to generate DNA sequences from exsiccata kept at O (Katriina Bendiksen, Oslo) and LUG (Neria Rohmer, Lugano), to colleagues who contributed DNA sequences from their own material: Segula Masaphy (Israel), Mustapha Isiloglu and Hayrunisa Bas Sermenli (Turkey), and by providing bibliographic and unpublished data: Philippe Callac (INRA, Bordeaux), Christophe Lecuru (LIP, Lille), Michael Loizides (Cyprus), Anne Molia (Oslo) and Nicolas Van Vooren (Lyon), to Roy Kristiansen (Fredrikstad) for detailed information about his collections and correspondences with Emile Jacquetant, to Francesco Bellu (Bolzano) for the translation of Bresadola's handwritten labels on collections of M. tridentina kept at Stockholm, and to David Hawksworth and Scott Redhead for pointing out the invalidity of Jacquetant's names and their expert assistance in resolving the mix-up of the original material of M. eximioides and M. eonicopapyracea. P. Clowez especially thanks all professional and amateur mycologists who sent him exsiccata and photographs of morels cited above (see SUPPLEMENTARY TABLE I). Photo credits for FIGS. 4 and SUPPLEMENTARY FIGS. I, 2 are: Philippe Clowez (4a, 4d, 4i, 4l and 4o), Tomas Illescas (4b), Renee Lebeuf (4c), Luis Romero de la Osa (4e, 4h), Hugh and Sandi Smith (4f), Chris Matherly (4g), Pierre-Arthur Moreau (4j, S1b, S1c and S1d), Luc Martin (4k), Claudine Michaud (4m), Nicolas Van Vooren (4n), Ramona Ubral Hedenberg (S1a) and Roy Kristiansen (S2a-m). DNA sequences were generated at the Service of Genetic Markers of UMR CEFE 5175. We thank Stacy Sink (ARS-USDA, Peoria, Illinois) and Xianghua Wang (SMNH, Stockholm) for generating some of the DNA sequence data and Nathane Orwig (ARS-USDA, Peoria, Illinois) for running them in the NCAUR DNA Core Facility, and Beatrice Boury (Univ. Lille 2) for assistance in the computerized management of LIP. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. This project was supported by the the grant "Diversite des champignons mycorhiziens des plantes" (DivMyc, to Marc-Andre Selosse) from the network Bibliotheque Du Vivant (BDV) funded by the CNRS, the Museum National d'Histoire Nature lle de Paris, the INRA and the CEA (Centre National de Sequencage). We are grateful to Marie-Pierre Dubois, Finn Kjellberg and Jean-Yves Rasplus for their support from the BDV program. NR 77 TC 8 Z9 8 U1 3 U2 27 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2015 VL 107 IS 2 BP 359 EP 382 DI 10.3852/14-166 PG 24 WC Mycology SC Mycology GA CE5RF UT WOS:000351892600010 PM 25550303 ER PT J AU Aoki, T Vaughan, MM McCormick, SP Busman, M Ward, TJ Kelly, A O'Donnell, K Johnston, PR Geiser, DM AF Aoki, Takayuki Vaughan, Martha M. McCormick, Susan P. Busman, Mark Ward, Todd J. Kelly, Amy O'Donnell, Kerry Johnston, Peter R. Geiser, David M. TI Fusarium dactylidis sp nov., a novel nivalenol toxin-producing species sister to F. pseudograminearum isolated from orchard grass (Dactylis glomerata) in Oregon and New Zealand SO MYCOLOGIA LA English DT Article DE cock's foot; crown rot; forage; Fusarium head blight; genotyping; morphology; mycotoxins; pathogenicity; phylogenetics; RPB1; RPB2; trichothecene; zearalenone ID HEAD BLIGHT PATHOGEN; GIBBERELLA-ZEAE; GENEALOGICAL CONCORDANCE; NORTH-AMERICA; GENE-CLUSTER; GRAMINEARUM; COMPLEX; CLADE; DEOXYNIVALENOL; RECOGNITION AB The B trichothecene toxin-producing clade (B clade) of Fusarium includes the etiological agents of Fusarium head blight, crown rot of wheat and barley and stem and ear rot of maize. B clade isolates also have been recovered from several wild and cultivated grasses, including Dactylis glomerata (orchard grass or cock's foot), one of the world's most important forage grasses. Two isolates from the latter host are formally described here as F. dactylidis. Phenotypically F. dactylidis most closely resembles F. ussurianum from the Russian Far East. Both species produce symmetrical sporodochial conidia that are similar in size and curved toward both ends. However, conidia of F. ussurianum typically end in a narrow apical beak while the apical cell of F. dactylidis is acute. Fusarium dactylidis produced nivalenol mycotoxin in planta as well as low but detectable amounts of the estrogenic mycotoxin zearalenone in vitro. Results of a pathogenicity test revealed that F. dactylidis induced mild head blight on wheat. C1 [Aoki, Takayuki] Natl Inst Agrobiol Sci, Genet Resources Ctr MAFF, Tsukuba, Ibaraki 3058602, Japan. [Vaughan, Martha M.; McCormick, Susan P.; Busman, Mark; Ward, Todd J.; Kelly, Amy; O'Donnell, Kerry] ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 60604 USA. [Johnston, Peter R.] Landcare Res Manaaki Whenua, Auckland 1142, New Zealand. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Aoki, T (reprint author), Natl Inst Agrobiol Sci, Genet Resources Ctr MAFF, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan. EM taoki@nias.affrc.go.jp NR 38 TC 6 Z9 6 U1 1 U2 10 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2015 VL 107 IS 2 BP 409 EP 418 DI 10.3852/14-213 PG 10 WC Mycology SC Mycology GA CE5RF UT WOS:000351892600012 PM 25550300 ER PT J AU Owen, SF Berl, JL Edwards, JW Ford, WM Wood, PB AF Owen, Sheldon F. Berl, Jacob L. Edwards, John W. Ford, W. Mark Wood, Petra Bohall TI Raccoon (Procyon lotor) Diurnal Den Use within an Intensively Managed Forest in Central West Virginia SO NORTHEASTERN NATURALIST LA English DT Article ID CENTRAL MISSISSIPPI; HABITAT SELECTION; ADULT RACCOONS; DISEASE SPREAD; TREE CAVITIES; AVAILABILITY; MESOPREDATOR; LANDSCAPE; ILLINOIS; AGE AB Intensive forest management may influence the availability of suitable den sites for large den-seeking species, such as Procyon lotor (Raccoon). As part of a Raccoon ecology study on an industrial forest in the Allegheny Mountains of central West Virginia, we radio-tracked 32 Raccoons to 175 diurnal den sites to determine relative use of dens that included cavity trees, rock dens, log piles, slash piles, and exposed limbs. Patterns of den use significantly differed between sexes and among seasons. Overall, we recorded 58 cavity dens in 12 tree species with 7 maternal dens found in 5 tree species. Raccoons selected larger-diameter den trees than available cavity trees and non-cavity trees. Because the abundance of suitable tree cavities is known to influence Raccoon densities and recruitment at fine spatial scales and female Raccoons in this study used tree cavities as maternal den sites, the continued harvest of large-diameter trees (i.e., those capable of developing den cavities) without replacement may impact Raccoon recruitment within intensively managed forests throughout the central Appalachians. C1 [Owen, Sheldon F.; Berl, Jacob L.; Edwards, John W.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Ford, W. Mark] USDA, No Res Stn, Forest Serv, Parsons, WV 26287 USA. [Wood, Petra Bohall] W Virginia Univ, US Geol Survey, West Virginia Cooperat Fish & Wildlife Res Unit, Morgantown, WV 26506 USA. RP Edwards, JW (reprint author), W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. EM jedwards@wvu.edu FU USDA Forest Service Northern Research Station; Division of Forestry and Natural Resources, West Virginia University; WV Cooperative Fish and Wildlife Research Unit US Geological Survey; MeadWestvaco Corporation; USDA/APHIS Wildlife Services; West Virginia Division of Natural Resources FX Funding was provided by USDA Forest Service Northern Research Station, Division of Forestry and Natural Resources, West Virginia University, WV Cooperative Fish and Wildlife Research Unit US Geological Survey, MeadWestvaco Corporation, USDA/APHIS Wildlife Services, and West Virginia Division of Natural Resources. We thank our field technicians, H. Brace and J. Adams for their hard work and long hours. We also thank J. Johnson, J. Rodrigue, and J. Crum for field assistance and G. Seidel for statistical guidance. The use of trade names or products does not constitute endorsement by the US Government. This is West Virginia Agriculture and Forestry Experiment Station Scientific Article No. 3237. NR 30 TC 1 Z9 1 U1 3 U2 13 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1092-6194 EI 1938-5307 J9 NORTHEAST NAT JI Northeast. Nat PD MAR PY 2015 VL 22 IS 1 BP 41 EP 52 DI 10.1656/045.022.0106 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CE1CZ UT WOS:000351550900006 ER PT J AU Abrams, SA Hawthorne, KM Pammi, M AF Abrams, Steven A. Hawthorne, Keli M. Pammi, Mohan TI A Systematic Review of Controlled Trials of Lower-Protein or Energy-Containing Infant Formulas for Use by Healthy Full-Term Infants SO ADVANCES IN NUTRITION LA English DT Review DE child growth; development; lactation; infant formula; obesity prevention; protein requirements ID RANDOMIZED CONTROLLED-TRIAL; WEIGHT-GAIN; FAT MASS; GROWTH; MILK; AGE; RATIO; METABOLISM; OVERWEIGHT; EFFICIENCY AB Infant formulas have historically been developed based on providing macronutrients at intake concentrations approximately matching the composition of human milk. In most countries, targets of 1.4-1.5 g of protein/dL and 20 kcal/oz (67-68 kcal/dL) have been set as the protein and energy concentrations for formulas during the first year of life, although this may be an overestimation of these contents. Recent introduction of lower-protein and -energy formulas in full-term infants led us to systematically review the literature for its effects on growth. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines, our inclusion criteria were studies that enrolled healthy full-term infants and evaluated lower-protein or lower-energy formula, reported anthropometric outcomes including weight and length, and followed infants for at least 6 mo. Six studies were eligible for inclusion. These studies varied in the content of nutrients provided in the intervention and control groups, by additional dietary components in the study groups, and the timing and length of the intervention, which limit their usefulness for interpreting newly introduced lower-protein and -energy formulas in the United States. These studies suggest adequate growth during infancy and early childhood with infant formulas with concentrations of protein and energy slightly below historical standards in the United States. Further long-term research is needed to assess the impact of the use of lower-protein and/or lower-energy products, especially for nutritionally at-risk populations such as preterm infants and infants who are born small for gestational age. C1 [Abrams, Steven A.; Hawthorne, Keli M.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Pammi, Mohan] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Sect Neonatol, Houston, TX 77030 USA. RP Abrams, SA (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM sabrams@bcm.edu OI Abrams, Steven/0000-0003-4972-9233 FU USDA/Agricultural Research Service [58-6250-6-001] FX Supported in part by federal funds from the USDA/Agricultural Research Service under Cooperative Agreement number 58-6250-6-001. NR 29 TC 5 Z9 5 U1 1 U2 13 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAR PY 2015 VL 6 IS 2 BP 178 EP 188 DI 10.3945/an.114.006379 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CE0GU UT WOS:000351483300004 PM 25770256 ER PT J AU Huang, LP Drake, VJ Ho, E AF Huang, Liping Drake, Victoria J. Ho, Emily TI Zinc SO ADVANCES IN NUTRITION LA English DT Editorial Material ID CELL-FUNCTION C1 [Huang, Liping] Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Drake, Victoria J.; Ho, Emily] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA. [Ho, Emily] Oregon State Univ, Moore Family Ctr, Corvallis, OR 97331 USA. RP Ho, E (reprint author), Oregon State Univ, Moore Family Ctr, Corvallis, OR 97331 USA. EM ho@oregonstate.edu NR 7 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAR PY 2015 VL 6 IS 2 BP 224 EP 226 DI 10.3945/an.114.006874 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CE0GU UT WOS:000351483300009 PM 25770261 ER PT J AU Beatty, TKM Tuttle, CJ AF Beatty, Timothy K. M. Tuttle, Charlotte J. TI Expenditure Response to Increases in In-Kind Transfers: Evidence from the Supplemental Nutrition Assistance Program SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE Labeling effect; Stimulus Bill; Supplemental Nutrition Assistance Program ID FOOD STAMP RECIPIENTS; EMPIRICAL-EVALUATION; CONSUMPTION; ECONOMICS; EQUATION; SNAP AB Economic theory predicts that households who receive less in Supplemental Nutrition Assistance Program benefits than they spend on food will treat SNAP benefits as if they were cash. However, empirical tests of these predictions draw different conclusions. In this study, we reexamine this question using recent increases in Supplemental Nutrition Assistance Program benefits, the largest of which was due to the American Recovery and Reinvestment Act of 2009. We find that increases in benefits cause households to increase their food budget share by more than would be predicted by theory. Results are robust to a host of specification tests. C1 [Beatty, Timothy K. M.] Univ Calif Davis, Dept Agr & Resource Econ, Davis, CA 95616 USA. [Tuttle, Charlotte J.] ERS, USDA, Washington, DC 20024 USA. RP Beatty, TKM (reprint author), Univ Calif Davis, Dept Agr & Resource Econ, 1 Shields Ave, Davis, CA 95616 USA. EM tbeatty@ucdavis.edu; cjtuttle@ers.usda.gov NR 29 TC 1 Z9 1 U1 1 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD MAR PY 2015 VL 97 IS 2 BP 390 EP 404 DI 10.1093/ajae/aau097 PG 15 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA CE0DI UT WOS:000351474300003 ER PT J AU Zeilinger, AR Olson, DM Raygoza, T Andow, DA AF Zeilinger, Adam R. Olson, Dawn M. Raygoza, Tania Andow, David A. TI Do Counts of Salivary Sheath Flanges Predict Food Consumption in Herbivorous Stink Bugs (Hemiptera: Pentatomidae)? SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE stylet; feeding preference; Pentatomidae; salivary sheath; haustellate ID NEZARA-VIRIDULA; FEEDING PREFERENCE; HETEROPTERA-PENTATOMIDAE; POPULATION-STRUCTURE; SEASONAL ABUNDANCE; STYLET SHEATH; COTTON; DAMAGE; SEED; TRANSMISSION AB Counts of salivary sheaths and salivary flanges have been widely used in studies of feeding behavior and crop damage of pestiferous stink bugs (Hemiptera: Pentatomidae) and other sheath-feeding Hemiptera. While salivary flanges can effectively predict crop damage by stink bugs, previous studies have assumed that food consumption (e.g., ingestion) and preference can also be inferred from flange data. Yet this assumption has remained untested. We investigated the relationship between the number of stink bug salivary flanges and consumption of cotton bolls for two important agricultural pest species: Nezara viridula (L.) and Euschistus servus (Say). We inferred food consumption rates from measures of relative growth rate and excreta quantity. To measure excreta, we quantified the color intensity, or chromaticity, of excreta using digital image analysis. We found a positive relationship between growth rate and the number of flanges for fifth instars of E. servus. However, we found no relationship between growth or excretion and the number of flanges for all stages of N. viridula and for E. servus adults. Our results indicate that counts of salivary flanges should not be used to infer food consumption or preference in studies on N. viridula and E. servus adults, but can be used in studies of E. servus nymphs. Species- and stage-specific differences in the relationship between consumption and salivary flanges suggests distinct feeding strategies among species and stages; such differences may be potentially important in determining crop damage from pestiferous stink bugs. C1 [Zeilinger, Adam R.] Univ Minnesota, Dept Entomol, Conservat Biol Program, St Paul, MN 55108 USA. [Olson, Dawn M.] USDA ARS, Crop Protect Res & Management Unit, Tifton, GA 31794 USA. [Raygoza, Tania] Univ Calif Riverside, Dept Entomol, Riverside, CA 92507 USA. [Andow, David A.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Andow, David A.] Univ Minnesota, Ctr Community Genet, St Paul, MN 55108 USA. RP Zeilinger, AR (reprint author), Univ Calif Berkeley, Berkeley Initiat Global Change Biol, 130 Mulford Hall 3114, Berkeley, CA 94720 USA. EM arz@berkeley.edu OI Zeilinger, Adam/0000-0002-4111-3568 FU National Research Initiative (NRI) from U.S. Department of Agriculture [2008-02409]; Integrative Graduate Education and Research Traineeship (IGERT) from U.S. National Science Foundation [0653827]; Thesis Research Grant; Doctoral Dissertation Fellowship from the Graduate School, University of Minnesota; Dayton-Wilkie Fund of the Bell Museum of Natural History, University of Minnesota FX This study was partially supported by an National Research Initiative (NRI) grant 2008-02409 from the U.S. Department of Agriculture to D.A.A., D.M.O., and John Ruberson, an Integrative Graduate Education and Research Traineeship (IGERT) grant 0653827 from U.S. National Science Foundation to the University of Minnesota, a Thesis Research Grant and a Doctoral Dissertation Fellowship from the Graduate School, University of Minnesota, to A.R.Z., and grants from the Dayton-Wilkie Fund of the Bell Museum of Natural History, University of Minnesota, to A.R.Z. We thank A. Hornbuckle and M. Smith for assisting the experiments; T. Brown, E. Rosengren, J. Skelton, and N. Lynch for assisting in digital image analysis; S. Acton for advising on the digital image analysis method; M. Daugherty for providing access to MATLAB and advice on statistical analyses; and P. Oikawa for advising on statistical analyses. NR 38 TC 2 Z9 2 U1 2 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 109 EP 116 DI 10.1093/aesa/sau011 PG 8 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200001 ER PT J AU Lee, JC Dreves, AJ Cave, AM Kawai, S Isaacs, R Miller, JC Van Timmeren, S Bruck, DJ AF Lee, Jana C. Dreves, Amy J. Cave, Adam M. Kawai, Shinji Isaacs, Rufus Miller, Jeffrey C. Van Timmeren, Steven Bruck, Denny J. TI Infestation of Wild and Ornamental Noncrop Fruits by Drosophila suzukii (Diptera: Drosophilidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE alternative host; fruit host; host range; invasive pest; spotted wing drosophila ID SPOTTED-WING DROSOPHILA; HOST; INSECTICIDES; CROPS; FIELD AB Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) is a pest of small fruits and cherries, and has also been noted to infest a variety of wild, ornamental, and uncultivated hosts. Identifying alternative hosts is critical for pest management. Research objectives were to: 1) survey fruits in the field for natural infestation of D. suzukii, 2) determine the susceptibility of fruits in laboratory no-choice studies, and 3) evaluate short-range preference between simultaneously ripe alternative hosts and cultivated fruits in laboratory choice studies. Field surveys identified new hosts or confirmed previously reported hosts including: Berberis aquifolium Pursh, Oregon grape; Cornus spp., dogwood; Cotoneaster lacteus W.W. Smith, milkflower cotoneaster; Elaeagnus umbellata Thunberg, Autumn olive; Frangula purshiana (de Candolle) A. Gray, cascara buckthorn; Lindera benzoin (L.) Blume, spicebush; Lonicera caerulea L., blue honeysuckle; Morus sp., mulberry; Phytolacca americana L., pokeweed; Prunus avium (L.) L., wild cherry; Prunus laurocerasus L., cherry laurel; Prunus lusitanica L., Portuguese laurel; Rubus armeniacus Focke, Himalaya blackberry; Rubus spectabilis Pursh, salmonberry; Sambucus nigra L., black elderberry; Sarcococca confusa Sealy, sweet box; Solanum dulcamara L., bittersweet nightshade; and Symphoricarpos albus (L.) S.F. Blake, snowberry. High fruit infestations were observed in S. confusa during April-May and Lonicera spp. in June before most commercial fruits ripen. From both field and laboratory studies, there was no evidence of susceptibility during the estimated ripe period Crataegus L. 'Autumn Glory,' hawthorn; Ilex crenata Thunberg, Japanese holly; Nandina domestica Thunberg, sacred bamboo; Rhaphiolepis umbellata (Thunberg) Makino, yeddo hawthorne; Rosa acicularis Lindley, prickly rose; Skimmia japonica Thunberg, Japanese skimmia; and Viburnum davidii Franchet, David's viburnum. Lastly, laboratory choice tests identified that several fall-ripening alternative hosts were more susceptible than 'Pinot noir' or 'Pinot gris' wine grapes. By understanding host use, growers can identify high-risk areas where coordinated action may reduce infestation of D. suzukii in crops. C1 [Lee, Jana C.; Cave, Adam M.; Bruck, Denny J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Dreves, Amy J.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Kawai, Shinji; Miller, Jeffrey C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Isaacs, Rufus; Van Timmeren, Steven] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. RP Lee, JC (reprint author), USDA ARS, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM jana.lee@ars.usda.gov FU Northwest Center for Small Fruits Research; Agricultural Research Foundation; MBG Marketing; Michigan State Horticultural Society; Project GREEEN; U.S. Department of Agriculture-Current Research Information System [USDA-CRIS] [5358-22000-037-00D]; U.S. Department of Agriculture-Specialty Crop Research Initiative [USDA-SCRI] [2010-51181-21167] FX We thank plant taxonomists Pat Breen and Richard Halse at Oregon State University for identification of plants. We thank Christina Fieland, Jesse Mindolovich, Danielle Selleck, and Jeff Wong for assistance with laboratory assays; and Jamie Christensen, Delilah Clement, Nick Davros, Emily Haas, Margaret Lund, Jacob Morden, Amanda Ohm, Tom Peerbolt, Helmut Riedl, Kalli Shades, Peter Shearer, Jon Wyma, and Adam Young with field surveys. We thank Megan Woltz for comments on the manuscript; and Amanda Lake, Victoria Skillman, and Carolyn Smullin for checking plant names. This project was funded by the Northwest Center for Small Fruits Research, Agricultural Research Foundation, MBG Marketing, the Michigan State Horticultural Society, Project GREEEN, U.S. Department of Agriculture-Current Research Information System [USDA-CRIS] 5358-22000-037-00D, and U.S. Department of Agriculture-Specialty Crop Research Initiative [USDA-SCRI] Grant 2010-51181-21167. NR 27 TC 40 Z9 40 U1 6 U2 78 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 117 EP 129 DI 10.1093/aesa/sau014 PG 13 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200002 ER PT J AU Young, OP AF Young, Orrey P. TI Activity Patterns, Associated Environmental Conditions, and Mortality of the Larvae of Tetracha (=Megacephala) carolina (Coleoptera: Cicindelidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Tetracha carolina; larva; burrow; day and night; sun and shade ID TIGER BEETLES; ARTHROPODS; MISSISSIPPI; SURVIVAL; BIOLOGY; HABITAT; DELTA; FOOD AB Larvae of the tiger beetle Tetracha (=Megacephala) carolina L. (Coleoptera: Cicindelidae) were observed weekly in a residential yard in Greenville, Washington Co., MS, from 21 September to the following 30 June. Cleared areas around building foundations during this period contained 194 burrows of first-, second-, and third-instar larvae, as represented by three different diameter holes. Larvae were at the top of their burrow and visible during periods both day and night. Larvae retreated down into their burrow for longer periods when the site was in the shade relative to when it was in the sun. Burrows were closed with a soil plug during rain, when larvae were molting, and during harsh environmental conditions. Larvae whose burrows had a southern exposure were active a month longer in the fall and a month earlier in the spring compared with northern exposure larvae, though emerged adults appeared in both areas in late May. The density of burrows at three sites averaged 1 per 36.5 cm(2). Mortality of the third and last larval stage at the largest site was 34.4%, with higher mortality of the earlier stages. C1 [Young, Orrey P.] USDA ARS, So Field Crop Insect Management Lab, Stoneville, MS 38776 USA. RP Young, OP (reprint author), 9496 Good Lion Rd, Columbia, MD 21045 USA. EM ory2pam@verizon.net NR 28 TC 0 Z9 0 U1 2 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 130 EP 136 DI 10.1093/aesa/sau017 PG 7 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200003 ER PT J AU Cooper, WR Swisher, KD Garczynski, SF Mustafa, T Munyaneza, JE Horton, DR AF Cooper, W. Rodney Swisher, Kylie D. Garczynski, Stephen F. Mustafa, Tariq Munyaneza, Joseph E. Horton, David R. TI Wolbachia Infection Differs Among Divergent Mitochondrial Haplotypes of Bactericera cockerelli (Hemiptera: Triozidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Cardinium; Wolbachia; tomato psyllid; Liberibacter psyllaurous; endosymbionts ID POTATO PSYLLID HAPLOTYPES; CYTOPLASMIC INCOMPATIBILITY; UNITED-STATES; POPULATIONS; SPECIATION; DROSOPHILA; PIPIENTIS; DYNAMICS; AMERICA; CROPS AB Four mitochondrial (cytrochrome oxidase I) haplotypes of the potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), have been identified in North America: western, central, northwestern, and southwestern. A recent study found that females of the northwestern haplotype mated by males of the western or central haplotypes failed to produce viable eggs. Our goal was to determine whether these patterns in reproductive incompatibility are associated with differences among haplotypes in the presence of cytoplasmic incompatibility-inducing bacteria, Wolbachia or Cardinium. Polymerase chain reaction (PCR) revealed that psyllids of the western and central haplotypes were both simultaneously infected with two strains of Wolbachia, but Wolbachia was not detected in psyllids of the northwestern haplotype. PCR using archived DNA from field-collected psyllids confirmed patterns in Wolbachia infection among the western, central, and northwestern haplotypes, and also indicated that Wolbachia was not detectable in psyllids of the southwestern haplotype, which were not available for the mating studies. Cardinium was not detected in psyllids regardless of haplotype. These results provide evidence that differences in Wolbachia infection may be the cause of cytoplasmic incompatibilities among sympatric yet biologically distinct populations of B. cockerelli that have highly divergent mitochondrial haplotypes. This knowledge will improve the interpretation of studies to assess interactions and biological differences among B. cockerelli haplotypes. C1 [Cooper, W. Rodney; Swisher, Kylie D.; Garczynski, Stephen F.; Munyaneza, Joseph E.; Horton, David R.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Mustafa, Tariq] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. RP Cooper, WR (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 FU Northwest Potato Research Consortium FX Piedad Acala, Pauline Anderson, Merilee Bayer, Debra Broers, Tara Culbertson, Heather Headrick, Millie Heidt, and Delia Ramos provided technical assistance. B. cockerelli samples were provided by Don Henne and Carrie Wohleb. Funding was provided by the Northwest Potato Research Consortium. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 37 TC 1 Z9 1 U1 2 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 137 EP 145 DI 10.1093/aesa/sau048 PG 9 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200004 ER PT J AU Morales-Ramos, JA Kay, S Rojas, MG Shapiro-Ilan, DI Tedders, WL AF Morales-Ramos, Juan A. Kay, Sasha Rojas, M. Guadalupe Shapiro-Ilan, David I. Tedders, W. Louis TI Morphometric Analysis of Instar Variation in Tenebrio molitor (Coleoptera: Tenebrionidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE yellow mealworm; development; frequency distribution; instar determination ID HEAD-CAPSULE WIDTHS; ENTOMOPATHOGENIC NEMATODES; FREQUENCY-DISTRIBUTION; NUTRIENT COMPOSITION; LARVAL INSTARS; SELF-SELECTION; GROWTH; DIETS; PENTATOMIDAE; PREY AB Measurements of head capsule, mandible, metanotum, and body weight were done on larvae of Tenebrio molitor L. (Coleoptera: Tenebrionide) from the second to the last instar. Instar number varied from 14 to 18, but 15 or 16 instars were the most common. The value of dimensional measurements was evaluated as a tool for instar determination and to improve understanding of instar variation in T. molitor. Three methods of analysis were used, including discriminant, cluster, and frequency distribution analyses. Stepwise regression analysis was used to determine the optimal combination of dimensional variables. Head capsule width, left mandible length, and body weight at the beginning of stadia were the most significant variables impacting instar. Discriminant analyses of instar using these three variables resulted in 47.74% misclassifications, indicating a high degree of dimensional overlapping among instars. Dimensional overlap and variability increased in older instars. Cluster 15-group analysis using measurements of head capsule width and mandible length resulted in the lowest level of group variability; however, there was low degree of correspondence between cluster groups and instars. Frequency distribution analysis of head capsule width revealed 11 peaks, but only the early 8 peaks corresponded with observed instars. All three methods of morphometric analysis failed to identify all instars of T. molitor correctly. Frequency distribution analysis provided the best match, but only among instars 2 to 10. It appears that instar variation in the larval development of T. molitor occurs after the 10th instar. A model of T. molitor developmental plasticity is proposed. C1 [Morales-Ramos, Juan A.; Rojas, M. Guadalupe] USDA ARS NBCL, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Kay, Sasha; Tedders, W. Louis] Southeastern Insectaries Inc, Perry, GA 31069 USA. [Shapiro-Ilan, David I.] USDA ARS, Southeastern Fruit & Nut Tree Res Lab, Byron, GA 31008 USA. RP Morales-Ramos, JA (reprint author), USDA ARS NBCL, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. EM Juan.moralesramos@ars.usda.gov FU U.S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) [2007-33610-18416] FX We thank the U.S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) for financing this research through the Small Business Innovation Research (SBIR) program (grant 2007-33610-18416/proposal number 2007-03695). We wish to make a posthumous dedication of this paper to one of our authors, Mr. W. Louis Tedders. NR 40 TC 1 Z9 1 U1 5 U2 23 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 146 EP 159 DI 10.1093/aesa/sau049 PG 14 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200005 ER PT J AU Perera, OP Gore, J Snodgrass, GL Jackson, RE Allen, KC Abel, CA Luttrell, RG AF Perera, Omaththage P. Gore, Jeff Snodgrass, Gordon L. Jackson, Ryan E. Allen, Kerry C. Abel, Craig A. Luttrell, Randall G. TI Temporal and Spatial Genetic Variability Among Tarnished Plant Bug (Hemiptera: Miridae) Populations in a Small Geographic Area SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Lygus; population genetics; microsatellite; simple sequence repeat; genetic structure ID MISSISSIPPI RIVER DELTA; MITOCHONDRIAL-DNA; LYGUS-LINEOLARIS; DIFFERENTIATION MEASURE; INSECTICIDE RESISTANCE; MICROSATELLITE MARKERS; UNSAMPLED POPULATIONS; PYRETHROID RESISTANCE; SAMPLED POPULATIONS; GOSSYPIUM-HIRSUTUM AB The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is an important pest of cotton that also feeds on other crops and many wild hosts. In the mid-South, where tarnished plant bugs in cotton are controlled exclusively with synthetic insecticides, infestations resulted in > 29% of all yield loss attributed to insect damage and the control costs exceeded US$10 per acre in 2013. They have developed resistance to the most commonly used insecticides. Estimations of gene flow and migration are important to understand the spread of resistance in tarnished plant bug populations. Here, we analyzed tarnished plant bugs collected from July to September, 2006, to estimate population genetic parameters using 13 microsatellite markers. Our data indicated that tarnished plant bug populations in the study area had undergone a population bottleneck and all loci deviated from Hardy-Weinberg equilibrium in one or more collections. Bayesian simulations and factorial correspondence analysis indicated the presence of two genetic clusters in the tarnished plant bug populations in the study area. Proportions of insects separated into the two genetic clusters changed from July to September. Genetic differentiation and reduced gene flow between populations were also observed. We postulate that while random genetic drift and gene flow may have contributed to the seasonal variations observed in the study populations, selection by insecticide sprays in cotton during 2005-2006 as well as other extrinsic factors could also have played a significant role in the temporal variation in genetic structure observed in the tarnished plant bug populations. C1 [Perera, Omaththage P.; Snodgrass, Gordon L.; Jackson, Ryan E.; Allen, Kerry C.; Luttrell, Randall G.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. [Gore, Jeff] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA. [Abel, Craig A.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Perera, OP (reprint author), USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. EM op.perera@ars.usda.gov FU Cotton Inc. [08-471] FX We thank Sheron Simpson, Genomics and Bioinformatics Research Unit, USDA-ARS, Stoneville, MS, for genotyping services, Charles Lanford, Southern Insect Management Research Unit (SIMRU), USDA-ARS, Stoneville, MS, for collecting insects, former SIMRU employees and student interns Torey Looft, Rachel Leininger, LeAndrea Williams, Laura Lindner, Nilushi Karunamuni, and Carol Bates for insect DNA extractions. We are also grateful to Drs. Tom Sappington (Corn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, IA), Wee Tek Tay (Commonwealth Scientific and Industrial Research Organization, Canberra, Australia), and Michael A. Caprio (Mississippi State University, Starkville, MS), for critically evaluating an earlier version of this manuscript. This research was partially supported by Cotton Inc. grant 08-471 to O.P.P. NR 68 TC 2 Z9 2 U1 2 U2 16 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 181 EP 192 DI 10.1093/aesa/sau016 PG 12 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200008 ER PT J AU Roehrdanz, RL Wichmann, SS AF Roehrdanz, Richard L. Wichmann, Sheila Sears TI 28S Ribosomal RNA Sequences Separate Five Prominent Lygus (Hemiptera: Miridae) Pest Species Into Three Species Clusters SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE tarnished plant bug; nuclear DNA; phylogeny ID MITOCHONDRIAL-DNA; TREES AB A segment of the nuclear 28S rRNA gene was compared among six species of Lygus [Lygus hesperus (Knight), Lygus keltoni (Schwartz), Lygus borealis (Kelton), Lygus elisus (Van Duzee), Lygus lineolaris (Palisot de Beauvois), and Lygus vanduzeei (Knight)]. The DNA sequences were separate into three main groups. The LL group contains L. lineolaris and L. vanduzeei. Group LBLE is composed of L. elisus and most of L. borealis. Group LH includes L. hesperus and most of L. keltoni. Some L. keltoni were part of the LBLE group and some L. borealis were part of the LH group. The 28S region does not contain sufficient genetic polymorphism to delineate species. The apparent polyphyly of L. borealis and L. keltoni could reflect historic interbreeding, recent development of a hybrid swarm, or highlight inadequacies of morphospecies identification. C1 [Roehrdanz, Richard L.; Wichmann, Sheila Sears] USDA ARS, Insect Genet & Biochem Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. RP Roehrdanz, RL (reprint author), USDA ARS, Insect Genet & Biochem Res Unit, Red River Valley Agr Res Ctr, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM richard.roehrdanz@ars.usda.gov NR 22 TC 0 Z9 0 U1 2 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2015 VL 108 IS 2 BP 211 EP 214 DI 10.1093/aesa/sav002 PG 4 WC Entomology SC Entomology GA CE0CE UT WOS:000351470200011 ER PT J AU Eckholm, BJ Huang, MH Anderson, KE Mott, BM DeGrandi-Hoffman, G AF Eckholm, Bruce J. Huang, Ming H. Anderson, Kirk E. Mott, Brendon M. DeGrandi-Hoffman, Gloria TI Honey bee (Apis mellifera) intracolonial genetic diversity influences worker nutritional status SO APIDOLOGIE LA English DT Article DE intracolonial genetic diversity; extreme polyandry; honey bee nutrition ID DIVISION-OF-LABOR; EXTREME POLYANDRY; MATING FREQUENCY; JUVENILE-HORMONE; PROTEIN DIETS; COLONIES; HYMENOPTERA; EVOLUTION; QUEEN; L. AB Honey bee queens mate with multiple males resulting in high intracolonial genetic diversity among nestmates; a reproductive strategy known as extreme polyandry. Several studies have demonstrated the adaptive significance of extreme polyandry for overall colony performance and colony growth. Colonies that are more genetically diverse collect more pollen than colonies with less diversity. However, the effects of intracolonial genetic diversity on worker nutritional status are unknown. We created colonies headed by queens instrumentally inseminated with sperm from either 1 or 20 drones, then compared protein consumption, digestion, and uptake among nestmates. We found that nurse bees from colonies with multiple-drone-inseminated (MDI) queens consumed more pollen, had lower amounts of midgut tissue protease, and invested more protein into larvae than nurse bees from single-drone-inseminated (SDI) queens. Pollen foragers from MDI colonies had significantly higher hemolymph protein concentration than pollen foragers from SDI colonies. Differences in hemolymph protein concentration between nurses and pollen foragers were significantly smaller among MDI colonies than among SDI colonies. While intracolonial genetic diversity is correlated with increased foraging, our results suggest that this relationship may be driven in part by the elevated resource demands of nurse bees in genetically diverse colonies that consume and distribute more protein in response to the social context within the hive. C1 [Huang, Ming H.] Eurofins Agrosci Serv Inc, Prospect Hill, NC 27314 USA. [Anderson, Kirk E.; Mott, Brendon M.; DeGrandi-Hoffman, Gloria] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. RP Eckholm, BJ (reprint author), 11025 Zylstra Rd, Coupeville, WA 98239 USA. EM bruce@eckholm.com OI Eckholm, Bruce/0000-0002-1661-925X NR 69 TC 2 Z9 2 U1 1 U2 28 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAR PY 2015 VL 46 IS 2 BP 150 EP 163 DI 10.1007/s13592-014-0311-4 PG 14 WC Entomology SC Entomology GA CD6TY UT WOS:000351223600003 ER PT J AU Cardenas-Garcia, S Diel, DG Susta, L Lucio-Decanini, E Yu, QZ Brown, CC Miller, PJ Afonso, CL AF Cardenas-Garcia, Stivalis Diel, Diego G. Susta, Leonardo Lucio-Decanini, Eduardo Yu, Qingzhong Brown, Corrie C. Miller, Patti J. Afonso, Claudio L. TI Development of an improved vaccine evaluation protocol to compare the efficacy of Newcastle disease vaccines SO BIOLOGICALS LA English DT Article DE NDV; Vaccine efficacy; Newcastle disease; Homologous vaccination; Mortality ID SHEDDING FOLLOWING VACCINATION; CELL-MEDIATED-IMMUNITY; GENETIC DIVERSITY; BIOLOGICAL CHARACTERIZATION; VIRUS-VACCINES; POULTRY; CHICKENS; CHALLENGE; GENOTYPE; STRAIN AB While there is typically 100% survivability in birds challenged with vNDV under experimental conditions, either with vaccines formulated with a strain homologous or heterologous (different genotype) to the challenge virus, vaccine deficiencies are often noted in the field. We have developed an improved and more stringent protocol to experimentally evaluate live NDV vaccines, and showed for the first time under experimental conditions that a statistically significant reduction in mortality can be detected with genotype matched vaccines. Using both vaccine evaluation protocols (traditional and improved), birds were challenged with a vNDV of genotype XIII and the efficacy of live heterologous (genotype II) and homologous (genotype XIII) NDV vaccines was compared. Under traditional vaccination conditions there were no differences in survival upon challenge, but the homologous vaccine induced significantly higher levels of antibodies specific to the challenge virus. With the more stringent challenge system (multiple vaccine doses and early challenge with high titers of vNDV), the birds administered the homologous vaccine had superior humoral responses, reduced clinical signs, and reduced mortality levels than those vaccinated with the heterologous vaccine. These results provide basis for the implementation of more sensitive methods to evaluate vaccine efficacy. Published by Elsevier Ltd on behalf of The International Alliance for Biological Standardization. C1 [Cardenas-Garcia, Stivalis; Susta, Leonardo; Yu, Qingzhong; Miller, Patti J.; Afonso, Claudio L.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. [Cardenas-Garcia, Stivalis; Brown, Corrie C.] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA USA. [Diel, Diego G.] S Dakota State Univ, Anim Dis Res & Diagnost Lab, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. [Lucio-Decanini, Eduardo] Invest Aplicada SA CV, Col Ctr, Puebla 75700, Mexico. RP Afonso, CL (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM stivaliscgs@gmail.com; diego.diel@sdstate.edu; leonardo.susta@ars.usda.gov; elucio@grupoidisa.com; qingzhong.yu@ars.usda.gov; corbrown@uga.edu; patti.miller@ars.usda.gov; claudio.afonso@ars.usda.gov FU Investigacion Aplicada, S.A. de C.V.; USDA-ARS CRADA [58-3K95-9-1380, 6612-32000-064-00D] FX This project was supported by Investigacion Aplicada, S.A. de C.V. and by USDA-ARS CRADA number 58-3K95-9-1380, CRIS number 6612-32000-064-00D. NR 43 TC 6 Z9 6 U1 0 U2 11 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1045-1056 EI 1095-8320 J9 BIOLOGICALS JI Biologicals PD MAR PY 2015 VL 43 IS 2 BP 136 EP 145 DI 10.1016/j.biologicals.2014.11.003 PG 10 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Pharmacology & Pharmacy GA CE2OM UT WOS:000351654900009 PM 25511007 ER PT J AU Wei, LQ McDonald, AG Stark, NM AF Wei, Liqing McDonald, Armando G. Stark, Nicole M. TI Grafting of Bacterial Polyhydroxybutyrate (PHB) onto Cellulose via In Situ Reactive Extrusion with Dicumyl Peroxide SO BIOMACROMOLECULES LA English DT Article ID RING-OPENING POLYMERIZATION; CROSS-LINKING; POLY(ETHYLENE OXIDE); CRYSTALLIZATION; COMPOSITES; FIBERS; POLYHYDROXYALKANOATES; COPOLYMERIZATION; NANOCRYSTALS; BEHAVIOR AB Polyhydroxybutyrate (PHB) was grafted onto cellulose fiber by dicumyl peroxide (DCP) radical initiation via in situ reactive extrusion. The yield of the grafted (cellulose-g-PHB) copolymer was recorded and grafting efficiency was found to be dependent on the reaction time and DCP concentration. The grafting mechanism was investigated by electron spin resonance (ESR) analysis and showed the presence of radicals produced by DCP radical initiation. The grafted copolymer structure was determined by nuclear magnetic resonance (NMR) spectroscopy. Scanning electronic microscopy (SEM) showed that the cellulose-g-PHB copolymer formed a continuous phase between the surfaces of cellulose and PHB as compared to cellulose-PHB blends. The relative crystallinity of cellulose and PHB were quantified from Fourier transform infrared (FTIR) spectra and X-ray diffraction (XRD) results, while the absolute degree of crystallinity was evaluated by differential scanning calorimetry (DSC). The reduction of crystallinity indicated the grafting reaction occurred not just in the amorphous region but also slightly in crystalline regions of both cellulose and PHB. The smaller crystal sizes suggested the brittleness of PHB was decreased. Thermogravimetric analysis (TGA) showed that the grafted copolymer was stabilized relative to PHB. By varying the reaction parameters the compositions (%PHB and %cellulose) of resultant cellulose-g-PHB copolymer are expected to be manipulated to obtain tunable properties. C1 [Wei, Liqing; McDonald, Armando G.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Renewable Mat Program, Moscow, ID 83844 USA. [Stark, Nicole M.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP McDonald, AG (reprint author), Univ Idaho, Dept Forest Rangeland & Fire Sci, Renewable Mat Program, Moscow, ID 83844 USA. EM armandm@uidaho.edu FU USDA-Forest Products Laboratory [08-JV-111111]; USDA-CSREES [2007-34158-17640] FX The authors would like to acknowledge (i) the financial support from a USDA-Forest Products Laboratory Grant 08-JV-111111, (ii) Dr. Alexander Blumenfeld for his technical help with ESR and NMR, (iii) USDA-CSREES Grant 2007-34158-17640 for supporting the DSC, and (iv) Thermo Scientific for the FTIR spectrometer. NR 40 TC 27 Z9 27 U1 19 U2 82 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1525-7797 EI 1526-4602 J9 BIOMACROMOLECULES JI Biomacromolecules PD MAR PY 2015 VL 16 IS 3 BP 1040 EP 1049 DI 10.1021/acs.biomac.5b00049 PG 10 WC Biochemistry & Molecular Biology; Chemistry, Organic; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA CD1NH UT WOS:000350841100039 PM 25664869 ER PT J AU Young, OP AF Young, Orrey P. TI SIZE RELATIONSHIPS, EARLY REPRODUCTIVE STATUS, AND MANDIBULAR WEAR IN ADULT TETRACHA (= MEGACEPHALA) CAROLINA (L.) (COLEOPTERA: CARABIDAE: CICINDELINAE) SO COLEOPTERISTS BULLETIN LA English DT Article DE tiger beetle; morphology; reproduction; mensural characters; Mississippi; old-field; teneral ID TIGER BEETLES COLEOPTERA; OLD-FIELD HABITAT; MISSISSIPPI; DELTA AB Adult Tetracha carolina (L.) were active during a 15-week period at an old-field site in Washington Co., Mississippi. Seven body measurements were obtained from 398 individuals captured by barrier pitfalls, with male measurements significantly different from females except for length of the mandibles. When females first appeared, some either contained developed eggs or had recently discharged eggs, even though they were still teneral. By the end of the 15-week period, many of the mandibles of both sexes were severely damaged, some broken to a mere stub. The possible relationship of early reproductive status and rapid damage to mandibles is discussed. C1 USDA ARS, So Field Crop Insect Management Lab, Stoneville, MS 38776 USA. RP Young, OP (reprint author), USDA ARS, So Field Crop Insect Management Lab, POB 346, Stoneville, MS 38776 USA. EM ory2pam@verizon.net NR 24 TC 0 Z9 0 U1 0 U2 5 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD MAR PY 2015 VL 69 IS 1 BP 167 EP 173 PG 7 WC Entomology SC Entomology GA CE0MH UT WOS:000351497700017 ER PT J AU Ogle, SM Davis, K Lauvaux, T Schuh, A Cooley, D West, TO Heath, LS Miles, NL Richardson, S Breidt, FJ Smith, JE McCarty, JL Gurney, KR Tans, P Denning, AS AF Ogle, Stephen M. Davis, Kenneth Lauvaux, Thomas Schuh, Andrew Cooley, Dan West, Tristram O. Heath, Linda S. Miles, Natasha L. Richardson, Scott Breidt, F. Jay Smith, James E. McCarty, Jessica L. Gurney, Kevin R. Tans, Pieter Denning, A. Scott TI An approach for verifying biogenic greenhouse gas emissions inventories with atmospheric CO2 concentration data SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE greenhouse gas emissions inventory; atmospheric inversion modeling; emissions verification; carbon cycle ID UNITED-STATES; CARBON-DIOXIDE; FLUXES; SINKS; LAND; INVERSIONS; EXCHANGE; MODELS; BUDGET; SEQUESTRATION AB Verifying national greenhouse gas (GHG) emissions inventories is a critical step to ensure that reported emissions data to the United Nations Framework Convention on Climate Change (UNFCCC) are accurate and representative of a country's contribution to GHG concentrations in the atmosphere. Furthermore, verifying biogenic fluxes provides a check on estimated emissions associated with managing lands for carbon sequestration and other activities, which often have large uncertainties. We report here on the challenges and results associated with a case study using atmospheric measurements of CO2 concentrations and inverse modeling to verify nationally-reported biogenic CO2 emissions. The biogenic CO2 emissions inventory was compiled for the Mid-Continent region of United States based on methods and data used by the US government for reporting to the UNFCCC, along with additional sources and sinks to produce a full carbon balance. The biogenic emissions inventory produced an estimated flux of -408 +/- 136 TgCO(2) for the entire study region, which was not statistically different from the biogenic flux of -478 +/- 146 TgCO(2) that was estimated using the atmospheric CO2 concentration data. At sub-regional scales, the spatial density of atmospheric observations did not appear sufficient to verify emissions in general. However, a difference between the inventory and inversion results was found in one isolated area of West-central Wisconsin. This part of the region is dominated by forestlands, suggesting that further investigation may be warranted into the forest Cstock or harvested wood product data from this portion of the study area. The results suggest that observations of atmospheric CO2 concentration data and inverse modeling could be used to verify biogenic emissions, and provide more confidence in biogenic GHG emissions reporting to the UNFCCC. C1 [Ogle, Stephen M.; Schuh, Andrew] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Ogle, Stephen M.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. [Davis, Kenneth; Lauvaux, Thomas; Miles, Natasha L.; Richardson, Scott] Penn State Univ, Dept Meteorol, University Pk, PA 16802 USA. [Schuh, Andrew] Colorado State Univ, Cooperat Inst Res Atmosphere, Ft Collins, CO 80523 USA. [Cooley, Dan; Breidt, F. Jay] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [West, Tristram O.] Pacific NW Natl Lab, Joint Global Change Res Inst, College Pk, MD 20740 USA. [Heath, Linda S.; Smith, James E.] USDA, Forest Serv, No Res Stn, Forest Sci Lab, Durham, NH 03824 USA. [McCarty, Jessica L.] Michigan Tech Res Inst, Ann Arbor, MI 48105 USA. [Gurney, Kevin R.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Tans, Pieter] NOAA, Earth Syst Res Lab, Global Monitoring Div, Boulder, CO 80305 USA. [Denning, A. Scott] Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. RP Ogle, SM (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Campus Delivery 1499, Ft Collins, CO 80523 USA. EM stephen.ogle@colostate.edu OI Ogle, Stephen/0000-0003-1899-7446 FU National Aeronautics and Space Administration (NASA), Terrestrial Ecology Program [NNX08AK08G, NNH12AU35I]; NASA; US Department of Energy; National Oceanic and Atmospheric Administration; US Department of Agriculture FX This synthesis and analysis was supported by a grant from the National Aeronautics and Space Administration (NASA), Terrestrial Ecology Program (NNX08AK08G to Colorado State University and NNH12AU35I to Department of Energy). The Mid-Continent study was supported by multiple agencies in the US government through the North American Carbon Program, particularly NASA, US Department of Energy, National Oceanic and Atmospheric Administration and US Department of Agriculture. Inventory and inversion results are archived in the NASA Distributed Active Archive Center at Oak Ridge National Laboratory. NR 67 TC 2 Z9 2 U1 3 U2 35 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2015 VL 10 IS 3 AR 034012 DI 10.1088/1748-9326/10/3/034012 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CD9KB UT WOS:000351416100014 ER PT J AU Haley, BJ Allard, M Brown, E Hovingh, E Karns, JS van Kessel, JS AF Haley, B. J. Allard, M. Brown, E. Hovingh, E. Karns, J. S. van Kessel, J. S. TI Molecular detection of the index case of a subclinical Salmonella Kentucky epidemic on a dairy farm SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Food safety; genomics; Salmonella ID RESISTANT SALMONELLA; ENTERICA; HERD; DISSEMINATION; PREVALENCE; SEROTYPES; CATTLE AB Salmonella enterica commonly colonizes the intestinal tract of cattle and is a leading cause of foodborne illness. A previously described investigation into the prevalence of S. enterica on a dairy farm revealed an 8-year-long asymptomatic S. enterica epidemic caused by serotypes Cerro and Kentucky in the lactating herd. To investigate the source of the S. Kentucky strains, the genomes of two S. Kentucky isolates were sequenced; one collected prior to the epidemic (2004) and one collected during the epidemic (2010). Comparative genomic analysis demonstrated significant polymorphisms between the two strains. PCR primers targeting unique and strain-specific regions were developed, and screening of the archived isolates identified the index case of the asymptomatic S. Kentucky epidemic as a heifer that was raised off-site and transported onto the study farm in 2005. Analysis of isolates collected from all heifers brought onto the farm demonstrated frequent re-introduction of clones of the epidemic strain suggesting transmission of pathogens between farms might occur repeatedly. C1 [Haley, B. J.; Karns, J. S.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD USA. [Allard, M.; Brown, E.; van Kessel, J. S.] US FDA, Div Microbiol, Ctr Food Safety & Nutr, College Pk, MD USA. [Hovingh, E.] Penn State Univ, Anim Diagnost Lab, University Pk, PA USA. RP van Kessel, JS (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, Serv,Room 202,Bldg 173, Beltsville, MD 20705 USA. EM joann.vankessel@ars.usda.gov NR 18 TC 1 Z9 1 U1 0 U2 5 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD MAR PY 2015 VL 143 IS 4 BP 682 EP 686 DI 10.1017/S0950268814001289 PG 5 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CD4NB UT WOS:000351058600002 PM 25703396 ER PT J AU Pokharel, R Marahatta, SP Handoo, ZA Chitwood, DJ AF Pokharel, Ramesh Marahatta, S. P. Handoo, Z. A. Chitwood, D. J. TI Nematode community structures in different deciduous tree fruits and grape in Colorado, USA and impact of organic peach and apple production practices SO EUROPEAN JOURNAL OF SOIL BIOLOGY LA English DT Article DE Abundance; Community index; Diversity; Fruit; Nematode; Production system ID PLANT-PARASITIC NEMATODES; SOIL FOOD-WEB; FUNCTIONAL DIVERSITY; AGRICULTURAL SOILS; CROPPING SYSTEMS; MANAGEMENT; INDICATORS; AGROECOSYSTEMS; POPULATION; DISEASE AB Although free-living nematodes improve soil health and crop production, plant-parasitic nematodes (PPNs) damage crop plants and reduce crop yield. In this investigation, surveys were conducted to determine the PPNs associated with Colorado tree fruit orchards, and research blocks were evaluated to study the effects of crops and production practices on nematode community structure, a soil health indicator. During the surveys, a total of 16 PPN genera were observed: eight in grape, eight each in pear, cherry and peach, seven in apple and 11 each in prune, plum and apricot soils. Xiphinema sp. (dagger nematode), Pratylenchus sp. (root lesion nematode) and Meloidogyne sp. (root-knot nematode) were the predominant nematodes. Based on the results, Xiphinema sp. can be considered an important and other two potentially important PPNs. In the research blocks, nematode diversity, abundance, and structural indices were compared in organic and conventional peach and apple production systems as well as the apple cultivars 'Honeycrisp' and 'Cameo'. Both peach and apple organic production systems had higher nematode diversity and significantly higher total nematodes than their conventional counterparts. A significant interaction of variety and production system in apple was observed for Ditylenchus and total fungivores, Helicotylenchus, Paratylenchus, and Discolaimium populations. In peach, nematode indices such as percentage of fungivores, fungivore/bacterivore and fungivore/(fungivore + bacterivore) ratios, richness, diversity, and Maturity Index were significantly higher in organic soils. In apple, the Enrichment Index and Structure Index were significantly higher in the organic production systems, whereas the Basal Index was significantly lower in organic production systems as compared to the conventional ones. Subsets of other parameters studied were not significantly different between the organic and conventional production system in both apple and peach crops. These results indicate that crop type as well as management practices influence nematode communities and that organic apple and peach production may be better than conventional apple and peach production with respect to improved nematode community structure. However, soil improvement such as addition of organic matter, especially in conventional production systems and in apple orchards, might improve soil health and productivity. Published by Elsevier Masson SAS. C1 [Pokharel, Ramesh] Maryland Dept Agr, Annapolis, MD 21401 USA. [Marahatta, S. P.] Univ Hawaii, Dept Plant Sci, Honolulu, HI 96822 USA. [Handoo, Z. A.; Chitwood, D. J.] ARS, USDA, Hematol Lab, Beltsville, MD 20705 USA. RP Pokharel, R (reprint author), Maryland Dept Agr, 50 Harry S Truman Pkwy, Annapolis, MD 21401 USA. EM ramesh.pokharel@maryland.gov FU Western Colorado Research Center of Colorado State University FX The first author acknowledges the Western Colorado Research Center of Colorado State University for support. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 42 TC 0 Z9 1 U1 7 U2 40 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 1164-5563 EI 1778-3615 J9 EUR J SOIL BIOL JI Eur. J. Soil Biol. PD MAR-APR PY 2015 VL 67 BP 59 EP 68 DI 10.1016/j.ejsobi.2015.02.003 PG 10 WC Ecology; Soil Science SC Environmental Sciences & Ecology; Agriculture GA CE1HC UT WOS:000351561700009 ER PT J AU Leonard, JM Medina, AL Neary, DG Tecle, A AF Leonard, Jackson M. Medina, Alvin L. Neary, Daniel G. Tecle, Aregai TI The Influence of Parent Material on Vegetation Response 15 years after the Dude Fire, Arizona SO FORESTS LA English DT Article ID PONDEROSA PINE FORESTS; NORTHERN ARIZONA; HERBACEOUS VEGETATION; LEHMANN LOVEGRASS; EXOTIC GRASSES; UNITED-STATES; RESTORATION; UNDERSTORY; MANAGEMENT; ECOSYSTEM AB This study examined the effects of two types of parent material, sandstone and limestone, on the response of vegetation growth after the 1990 Dude Fire in central Arizona. The operating hypothesis of the study was that, given the right conditions, severe wildfire can trigger vegetation type conversion. Overall, three patterns emerged: (1) oak density increased by 413% from unburned sites to burned sites, with the highest densities occurring on sandstone soils; (2) weeping lovegrass (Eragrostis curvula Nees), a very aggressive non-native grass species seeded after the fire, now makes up 81% of the total herbaceous cover in the burned area; and (3) bare ground cover is 150% higher and litter cover is 50% lower in the burned area. Soil analysis was not definitive enough to differentiate impacts between parent materials however it was useful in quantifying the long-term impact of the fire on soils. The results of this study support the idea that catastrophic fire events can trigger vegetation type conversion and that perennial, non-native species used in rehabilitation efforts can persist within the ecosystem for long periods of time. Hence, the recovery period needed for the Dude Fire site to revert back to a pine-oak dominated forest could be on the scale of many decades to centuries. C1 [Leonard, Jackson M.; Neary, Daniel G.] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Medina, Alvin L.] US Forest Serv, USDA, Flagstaff, AZ 86005 USA. [Tecle, Aregai] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. RP Neary, DG (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM jleonard@fs.fed.us; almedina777@hotmail.com; dneary@fs.fed.us; aregai.tecle@nau.edu FU Payson Ranger District; USDA Forest Service National Fire Plan, Rocky Mountain Research Station, Fort Collins, Colorado; Air, Water, and Aquatic Environments research program; Northern Arizona University, School of Forestry FX The authors would like to thank the staff of the Tonto National Forest Supervisor's Office and the Payson Ranger District for their support of this research. The research was funded through the USDA Forest Service National Fire Plan, Rocky Mountain Research Station, Fort Collins, Colorado, and the Air, Water, and Aquatic Environments research program. Support from Northern Arizona University, School of Forestry is gratefully acknowledged. NR 64 TC 0 Z9 0 U1 3 U2 5 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAR PY 2015 VL 6 IS 3 BP 613 EP 635 DI 10.3390/f6030613 PG 23 WC Forestry SC Forestry GA CE5IA UT WOS:000351863600005 ER PT J AU Kindiger, B AF Kindiger, Bryan TI Transfer of the perfect flower trait from Poa secunda to Poa arachnifera SO GRASSLAND SCIENCE LA English DT Article DE Dioecious; perfect flowered; Poa ID REGISTRATION; BLUEGRASS; PLANTS AB A Texas bluegrass (Poa arachnifera) population has been developed that successfully integrates the perfect flower trait from Poa secunda through interspecific hybridization. The resulting perfect flowered P.arachnifera population is perennial, rhizomatous and lacks the cobweb-like hairs that are a typical characteristic of the P.arachnifera parent. Inheritance of the perfect flowered trait suggests a relatively simple inheritance, perhaps allelic to the segregation of staminate and pistillate individuals in other dioecious systems. This material may have applications as a clean seeded, perfect flowered P.arachnifera cultivar; or as a germplasm resource in a P.arachnifera breeding program. C1 USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Kindiger, B (reprint author), USDA ARS, Grazinglands Res Lab, 7207 West Cheyenne St, El Reno, OK 73036 USA. EM bryan.kindiger@ars.usda.gov NR 9 TC 0 Z9 0 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1744-6961 EI 1744-697X J9 GRASSL SCI JI Grassl. Sci. PD MAR PY 2015 VL 61 IS 1 BP 41 EP 44 DI 10.1111/grs.12068 PG 4 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA CD9GF UT WOS:000351405300007 ER PT J AU Ashby, RD Solaiman, DKY Strahan, GD Levine, AC Nomura, CT AF Ashby, Richard D. Solaiman, Daniel K. Y. Strahan, Gary D. Levine, Alex C. Nomura, Christopher T. TI Methanol-induced chain termination in poly(3-hydroxybutyrate) biopolymers: Molecular weight control SO INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES LA English DT Article DE Poly(3-hydroxybutyrate); Methanol; Methoxy end-capping; Molecular weight control ID CO-PRODUCT STREAM; PSEUDOMONAS-OLEOVORANS; LENGTH POLY(HYDROXYALKANOATES); POLYHYDROXYALKANOATES PHAS; CRUDE GLYCEROL; WASTE GLYCEROL; BIOSYNTHESIS; POLYESTERS; ACID; POLY(BETA-HYDROXYALKANOATES) AB A systematic study was performed to demonstrate the impact of methanol (MeOH) on poly(3-hydroxybutyrate) (PHB) synthesis and molecular weight (MW). Glycerine was used as the primary carbon source with varying concentrations of MeOH. Methanol retarded but did not completely inhibit growth and PHB production in Pseudomonas oleovorans. Proton NMR analysis revealed that the PHB polymers were end-capped with methoxy chemical groups causing MW reductions. The MW decreases were contingent upon the initial MeOH media concentration and the duration of the fermentations. The largest impact occurred at an initial MeOH concentration of 0.10% (w/v) where the number average molecular weights (M-n) decreased by 39%, 55%, and 72% in the 48, 72 and 96h cultures, respectively. Diffusion ordered NMR spectroscopy revealed a diffusivity (D) increase in the smaller molecular weight polymers with the PHB synthesized in the presence of 0.85% MeOH (72 h post-inoculation) having a D value of 0.66 x 10(-10) m(2)/s. Diffusivity increases indicate a reduction in hydrodynamic radii (R-hz) consistent with shorter chain-lengths. Crude glycerine from the biodiesel production process has been used as an inexpensive fermentation feedstock for polyhydroxyalkanoate (PHA) synthesis but its composition is facility-dependent. This information will be vital to tailor PHA properties to specific applications. Published by Elsevier B.V. C1 [Ashby, Richard D.; Solaiman, Daniel K. Y.; Strahan, Gary D.] Agr Res Serv, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Levine, Alex C.; Nomura, Christopher T.] SUNY Coll Environm Sci & Forestry, Dept Chem, Syracuse, NY 13210 USA. RP Ashby, RD (reprint author), Agr Res Serv, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Rick.Ashby@ARS.USDA.GOV OI Nomura, Christopher/0000-0001-6981-5679 FU NSF-CBET [1263905] FX The authors thank Nicole Crocker and Jennifer Thomas for their technical assistance throughout this study. Gel permeation chromatography studies were supported by NSF-CBET 1263905 awarded to C.T. Nomura. NR 48 TC 3 Z9 4 U1 2 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0141-8130 EI 1879-0003 J9 INT J BIOL MACROMOL JI Int. J. Biol. Macromol. PD MAR PY 2015 VL 74 BP 195 EP 201 DI 10.1016/j.ijbiomac.2014.12.026 PG 7 WC Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA CD7CO UT WOS:000351248600026 PM 25542165 ER PT J AU Fowler, J Kakani, R Haq, A Byrd, JA Bailey, CA AF Fowler, J. Kakani, R. Haq, A. Byrd, J. A. Bailey, C. A. TI Growth promoting effects of prebiotic yeast cell wall products in starter broilers under an immune stress and Clostridium perfringens challenge SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE yeast cell wall; mannanoligosaccharide; broiler; prebiotic; growth promotion ID MANNAN-OLIGOSACCHARIDES; NECROTIC ENTERITIS; BIO-MOS; PERFORMANCE; MICROFLORA; CHICKENS; DIETS AB This study was designed to investigate the growth promoting effects of supplementing different sources and concentrations of prebiotic yeast cell wall (YCW) products containing mannanoligosaccharides in starter broilers under an immune stress and Clostridium perfringens challenge. Through a series of 6 individual studies either 240 or 288 newly hatched chicks were randomly distributed to specific dietary treatments. All birds received a commercial infectious bursa disease vaccine at 10 or 15 d age followed by an orally administered Clostridium perfringens challenge on d 15, 16, and 17; or 18, 19, and 20. Weekly BW, feed consumption, and daily mortality were recorded per pen. Each study was terminated after 21 d. Pooled data analysis of all studies revealed no effect between different product sources of YCW. Products from both sources produced a significant improvement in growth rate compared to control birds fed no YCW. However, a blend of twoYCWproducts showed an approximate 15% improvement in growth rate and a 10% reduction in FCR. The optimum dose of any YCW product among those tested was determined to be approximately 250 ppm. Prebiotic YCW additives increased BW and improved feed conversion and may be considered as alternative growth promoters for starting broilers. C1 [Fowler, J.; Kakani, R.; Haq, A.; Bailey, C. A.] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77845 USA. [Byrd, J. A.] USDA ARS, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Fowler, J (reprint author), Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77845 USA. EM fowlerj22@tamu.edu NR 24 TC 4 Z9 4 U1 3 U2 17 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD MAR PY 2015 VL 24 IS 1 BP 66 EP 72 DI 10.3382/japr/pfv010 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CD7XK UT WOS:000351308600009 ER PT J AU Powell, JM Rotz, CA AF Powell, J. M. Rotz, C. A. TI Measures of Nitrogen Use Efficiency and Nitrogen Loss from Dairy Production Systems SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID DIETARY CRUDE PROTEIN; RUMEN-PROTECTED METHIONINE; MILK UREA NITROGEN; AMMONIA EMISSIONS; FERTILIZER NITROGEN; HERD MODEL; FARMS; WISCONSIN; COWS; EXCRETION AB In dairy production systems, tradeoffs can occur between fertilizer N applications and crop N use, feed N consumption and manure N excretion, and environmental impacts. This paper examines (i) how stocking rates affect N imports and management on dairy farms, N use efficiency (NUE; i. e., the amount of applied N incorporated into product N), and N loss; (ii) how reductions in fertilizer N and feed N may affect crop and milk production, NUE, and N loss; and (iii) why tradeoffs in N use outcomes should be considered when attempting to enhance overall NUE and reduce N loss. The Integrated Farm Simulation Model simulations of two representative dairy farm types and analyses of regional studies, long-term field experiments, and cow nutrition trials were used to demonstrate that (i) stocking rate affects cropping patterns, fertilizer and feed imports, and N loss; (ii) although fertilizer N reductions of 20 kg N ha(-1) may reduce slightly the crude protein (CP) content of corn silage (which would require purchase of additional CP supplements), this practice should not affect long-term corn yield but would reduce nitrate (NO3) and nitrous oxide (N2O) losses by 13 to 38%; (iii) dietary CP could be reduced on many dairy farms, which would not affect milk production but would reduce ammonia (NH3) and N2O emissions by 15 to 43%; and (iv) greater recognition of the tradeoffs in N use and N loss are needed to provide a better understanding of the potentials to enhance overall NUE and reduce environmental N loss from dairy production systems. C1 [Powell, J. M.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Rotz, C. A.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Powell, JM (reprint author), ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM mark.powell@ars.usda.gov FU United States Department of Agriculture National Institute for Food and Agriculture [2013-68002-20525]; NSF Research Coordination Network [DEB-1049744]; Soil Science Society of America; American Geophysical Union; International Plant Nutrition Institute; Fertilizer Institute; International Nitrogen Initiative FX In recognition of Dr. Michael Russelle's accomplishments and retirement, a pioneer in nitrogen research in dairy production systems. This study was partially funded through the United States Department of Agriculture National Institute for Food and Agriculture-supported projects, Dairy Coordinated Agricultural Project (CAP): Climate Change, Mitigation, and Adaptation in Dairy Production Systems of the Great Lakes Region (Award No. 2013-68002-20525). This work resulted from a conference supported by NSF Research Coordination Network award DEB-1049744 and by the Soil Science Society of America, the American Geophysical Union, The International Plant Nutrition Institute, The Fertilizer Institute, and the International Nitrogen Initiative. NR 55 TC 8 Z9 8 U1 5 U2 46 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 336 EP 344 DI 10.2134/jeq2014.07.0299 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000004 PM 26023953 ER PT J AU Osmond, DL Hoag, DLK Luloff, AE Meals, DW Neas, K AF Osmond, Deanna L. Hoag, Dana L. K. Luloff, Al E. Meals, Donald W. Neas, Kathy TI Farmers' Use of Nutrient Management: Lessons from Watershed Case Studies SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID GULF-OF-MEXICO; NITRATE CONCENTRATIONS; MISSISSIPPI RIVER; NITROGEN RATE; LAND-USE; CORN; CONSERVATION; CONSEQUENCES; PHOSPHORUS; DELIVERY AB Nutrient enrichment of water resources has degraded coastal waters throughout the world, including in the United States (e. g., Chesapeake Bay, Gulf of Mexico, and Neuse Estuary). Agricultural nonpoint sources have significant impacts on water resources. As a result, nutrient management planning is the primary tool recommended to reduce nutrient losses from agricultural fields. Its effectiveness requires nutrient management plans be used by farmers. There is little literature describing nutrient management decision-making. Here, two case studies are described that address this gap: (i) a synthesis of the National Institute of Food and Agriculture, the Conservation Effects Assessment Project, and (ii) field surveys from three nutrient-impaired river basins/watersheds in North Carolina (Neuse, Tar-Pamlico, and Jordan Lake drainage areas). Results indicate farmers generally did not fully apply nutrient management plans or follow basic soil test recommendations even when they had them. Farmers were found to be hesitant to apply N at university-recommended rates because they did not trust the recommendations, viewed abundant N as insurance, or used recommendations made by fertilizer dealers. Exceptions were noted when watershed education, technical support, and funding resources focused on nutrient management that included easing management demands, actively and consistently working directly with a small group of farmers, and providing significant resource allocations to fund agency personnel and cost-share funds to farmers. Without better dialogue with farmers and meaningful investment in strategies that reward farmers for taking what they perceive as risks relative to nutrient reduction, little progress in true adoption of nutrient management will be made. C1 [Osmond, Deanna L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Hoag, Dana L. K.] Colorado State Univ, Agr & Resource Econ, Ft Collins, CO 80523 USA. [Luloff, Al E.] Penn State Univ, Agr Econ Sociol & Educ, University Pk, PA 16802 USA. [Meals, Donald W.] Ice Nine Environm Consulting, Burlington, VT 05401 USA. [Neas, Kathy] NASS, USDA, Raleigh, NC 27601 USA. RP Osmond, DL (reprint author), N Carolina State Univ, Dept Soil Sci, Box 7619, Raleigh, NC 27695 USA. EM deanna_osmond@ncsu.edu FU USEPA; USDA NIFA; NRCS [2007-51130-18575]; NSF Research Coordination Network [DEB-1049744]; Soil Science Society of America; American Geophysical Union; International Plant Nutrition Institute; Fertilizer Institute; International Nitrogen Initiative FX The North Carolina watershed studies were funded by three separate USEPA grants as pass-through funds to the NC Department of Environment and Natural Resources. The NIFA-CEAP watershed study synthesis was funded by USDA NIFA and NRCS (Agreement No. 2007-51130-18575). This work resulted from a conference supported by NSF Research Coordination Network award DEB-1049744 and by the Soil Science Society of America, the American Geophysical Union, The International Plant Nutrition Institute, The Fertilizer Institute, and the International Nitrogen Initiative. NR 49 TC 9 Z9 9 U1 7 U2 39 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 382 EP 390 DI 10.2134/jeq2014.02.0091 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000008 PM 26023957 ER PT J AU Jarecki, MK Hatfield, JL Barbour, W AF Jarecki, Marek K. Hatfield, Jerry L. Barbour, Wiley TI Modeled Nitrous Oxide Emissions from Corn Fields in Iowa Based on County Level Data SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CARBON-DIOXIDE; AGRICULTURAL SOILS; CROPPING SYSTEM; USE EFFICIENCY; N2O EMISSIONS; GAS EMISSIONS; MANAGEMENT; FERTILIZER; TILLAGE; ROTATION AB The U. S. Corn Belt area has the capacity to generate high nitrous oxide (N2O) emissions due to medium to high annual precipitation, medium-to heavy-textured soils rich in organic matter, and high nitrogen (N) application rates. The purpose of this work was to estimate N2O emissions from cornfields in Iowa at the county level using the DeNitrification-DeComposition (DNDC) model and to compare the DNDC N2O emission estimates with available results from field experiments. All data were acquired for 2007 to 2011. Weather Underground Network and the Iowa State University Iowa Soil Properties and Interpretation Database 7.3 were the data sources for DNDC inputs and for computing county soil parameters. The National Agriculture Statistic Service 5-yr averages for corn yield data were used to establish ex post fertilizer N input at the county level. The DNDC output suggested county-wide N2O emissions in Iowa ranged from 2.2 kg N2O-N ha(-1) yr(-1) in south-central to 4.6 to 4.7 kg N2O-N ha(-1) yr(-1) in north-central and eastern Iowa counties. In northern districts, the average direct N2O emissions were 3.2, 4.4, and 3.6 kg N2O-N ha(-1) yr(-1) for west, central, and east, respectively. In central districts, average N2O emissions were 3.5, 3.9, and 3.4 kg N2O-N ha(-1) yr(-1) for west, central, and east, respectively. For southern districts, N2O emissions were 3.5, 2.6, and 3.1 kg N2O-N ha(-1) yr(-1) for west, central, and east, respectively. Direct N2O emissions estimated by the DNDC model were 1.93% of N fertilizer input to corn fields in Iowa, with values ranging from 1.66% in the northwest cropping district to 2.25% in the north-central cropping district. These values are higher than the average 1% loss rate used in the IPCC Tier 1 approach. C1 [Jarecki, Marek K.; Barbour, Wiley] Camco Clean Energy, Lone Tree, CO 80124 USA. [Hatfield, Jerry L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Jarecki, MK (reprint author), Camco Clean Energy, 9360 Stn St, Lone Tree, CO 80124 USA. EM majarek@yahoo.com FU NSF Research Coordination Network [DEB-1049744]; Soil Science Society of America; American Geophysical Union; International Plant Nutrition Institute; Fertilizer Institute; International Nitrogen Initiative; Camco Clean Energy; Cventure LLC. FX This work resulted from a conference supported by NSF Research Coordination Network award DEB-1049744 and by the Soil Science Society of America, the American Geophysical Union, The International Plant Nutrition Institute, The Fertilizer Institute, and the International Nitrogen Initiative. The authors gratefully acknowledge the contributions made by Mike Bess and Adam Penque as well as financial and in-kind support from Camco Clean Energy and Cventure LLC. NR 55 TC 5 Z9 5 U1 4 U2 30 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 431 EP 441 DI 10.2134/jeq2014.03.0100 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000013 PM 26023962 ER PT J AU Kleinman, PJA Smith, DR Bolster, CH Easton, ZM AF Kleinman, Peter J. A. Smith, Douglas R. Bolster, Carl H. Easton, Zachary M. TI Phosphorus Fate, Management, and Modeling in Artificially Drained Systems SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID WATER-QUALITY PROTECTION; AGRICULTURAL DRAINAGE; TILE DRAINAGE; COASTAL-PLAIN; DITCHES; TRANSPORT; SOILS; LOSSES AB Phosphorus (P) losses in agricultural drainage waters, both surface and subsurface, are among the most difficult form of nonpoint source pollution to mitigate. This special collection of papers on P in drainage waters documents the range of field conditions leading to P loss in drainage water, the potential for drainage and nutrient management practices to control drainage losses of P, and the ability of models to represent P loss to drainage systems. A review of P in tile drainage and case studies from North America, Europe, and New Zealand highlight the potential for artificial drainage to exacerbate watershed loads of dissolved and particulate P via rapid, bypass flow and shorter flow path distances. Trade-offs are identified in association with drainage intensification, tillage, cover crops, and manure management. While P in drainage waters tends to be tied to surface sources of P (soil, amendments or vegetation) that are in highest concentration, legacy sources of P may occur at deeper depths or other points along drainage flow paths. Most startling, none of the major fate-and-transport models used to predict management impacts on watershed P losses simulate the dominant processes of P loss to drainage waters. Because P losses to drainage waters can be so difficult to manage and to model, major investment are needed (i) in systems that can provide necessary drainage for agronomic production while detaining peak flows and promoting P retention and (ii) in models that can adequately describe P loss to drainage waters. C1 [Kleinman, Peter J. A.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Smith, Douglas R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Bolster, Carl H.] ARS, USDA, Food Anim Environm Syst Res Unit, Bowling Green, KY 42104 USA. [Easton, Zachary M.] Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA. RP Kleinman, PJA (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM peter.kleinman@ars.usda.gov NR 47 TC 15 Z9 15 U1 10 U2 59 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 460 EP 466 DI 10.2134/jeq2015.02.0090 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000016 PM 26023965 ER PT J AU Smith, DR King, KW Johnson, L Francesconi, W Richards, P Baker, D Sharpley, AN AF Smith, Douglas R. King, Kevin W. Johnson, Laura Francesconi, Wendy Richards, Pete Baker, Dave Sharpley, Andrew N. TI Surface Runoff and Tile Drainage Transport of Phosphorus in the Midwestern United States SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID AGRICULTURAL DRAINAGE; WATER-QUALITY; NITROGEN; TILLAGE; LOSSES; FLOW AB The midwestern United States offers some of the most productive agricultural soils in the world. Given the cool humid climate, much of the region would not be able to support agriculture without subsurface (tile) drainage because high water tables may damage crops and prevent machinery usage in fields at critical times. Although drainage is designed to remove excess soil water as quickly as possible, it can also rapidly transport agrochemicals, including phosphorus (P). This paper illustrates the potential importance of tile drainage for P transport throughout the midwestern United States. Surface runoff and tile drainage from fields in the St. Joseph River Watershed in northeastern Indiana have been monitored since 2008. Although the traditional concept of tile drainage has been that it slowly removes soil matrix flow, peak tile discharge occurred at the same time as peak surface runoff, which demonstrates a strong surface connection through macropore flow. On our research fields, 49% of soluble P and 48% of total P losses occurred via tile discharge. Edge-of-field soluble P and total P areal loads often exceeded watershed-scale areal loadings from the Maumee River, the primary source of nutrients to the western basin of Lake Erie, where algal blooms have been a pervasive problem for the last 10 yr. As farmers, researchers, and policymakers search for treatments to reduce P loading to surface waters, the present work demonstrates that treating only surface runoff may not be sufficient to reach the goal of 41% reduction in P loading for the Lake Erie Basin. C1 [Smith, Douglas R.; Francesconi, Wendy] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47906 USA. [King, Kevin W.] ARS, USDA, Soil Drainage Res Unit, Columbus, OH 43210 USA. [Johnson, Laura; Richards, Pete; Baker, Dave] Heidelberg Univ, Natl Ctr Water Qual Res, Tiffin, OH 44883 USA. [Sharpley, Andrew N.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. RP Smith, DR (reprint author), ARS, USDA, Natl Soil Eros Res Lab, 275 S Russell St, W Lafayette, IN 47906 USA. EM douglas.r.smith@ars.usda.gov NR 25 TC 39 Z9 39 U1 15 U2 97 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 495 EP 502 DI 10.2134/jeq2014.04.0176 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000019 PM 26023968 ER PT J AU Kleinman, PJA Church, C Saporito, LS McGrath, JM Reiter, MS Allen, AL Tingle, S Binford, GD Han, K Joern, BC AF Kleinman, Peter J. A. Church, Clinton Saporito, Lou S. McGrath, Josh M. Reiter, Mark S. Allen, Arthur L. Tingle, Shawn Binford, Greg D. Han, Kun Joern, Brad C. TI Phosphorus Leaching from Agricultural Soils of the Delmarva Peninsula, USA SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID COASTAL-PLAIN SOILS; MANURE APPLICATION; WATER-FLOW; DRAINAGE DITCHES; TILE DRAINS; TRANSPORT; SUBSURFACE; LOSSES; MANAGEMENT; GRASSLAND AB Leaching of phosphorus (P) mobilizes edaphic and applied sources of P and is a primary pathway of concern in agricultural soils of the Delmarva Peninsula, which defines the eastern boundary of the eutrophic Chesapeake Bay. We evaluated P leaching before and after poultry litter application from intact soil columns (30 cm diameter x 50 cm depth) obtained from low-and high-P members of four dominant Delmarva Peninsula soils. Surface soil textures ranged from fine sand to silt loam, and Mehlich-3 soil P ranged from 64 to 628 mg kg(-1). Irrigation of soil columns before litter application pointed to surface soil P controls on dissolved P in leachate (with soil P sorption saturation providing a stronger relationship than Mehlich-3 P); however, strong relationships between P in the subsoil (45-50 cm) and leachate P concentrations were also observed (r(2) = 0.61-0.73). After poultry litter application (4.5 Mg ha(-1)), leachate P concentrations and loads increased significantly for the finest-textured soils, consistent with observations that well-structured soils have the greatest propensity to transmit applied P. Phosphorus derived from poultry litter appeared to contribute 41 and 76% of total P loss in leachate from the two soils with the finest textures. Results point to soil P, including P sorption saturation, as a sound metric of P loss potential in leachate when manure is not an acute source of P but highlight the need to factor in macropore transport potential to predict leaching losses from applied P sources. C1 [Kleinman, Peter J. A.; Church, Clinton; Saporito, Lou S.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [McGrath, Josh M.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Reiter, Mark S.] Virginia Tech Univ, Eastern Shore Agr Res & Extens Ctr, Painter, VA 23420 USA. [Allen, Arthur L.] Univ Maryland Eastern Shore, Dept Agr, Princess Anne, MD 21853 USA. [Tingle, Shawn] Univ Delaware, Carvel Res & Educ Ctr, Georgetown, DE 19947 USA. [Binford, Greg D.] Wilbur Ellis Co, Zionsville, IN 46077 USA. [Han, Kun] Shandong Agr Univ, Agron Coll, Tai An, Shandong, Peoples R China. [Joern, Brad C.] Purdue Univ, Dept Agr, W Lafayette, IN 47907 USA. RP Kleinman, PJA (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM peter.kleinman@ars.usda.gov NR 46 TC 6 Z9 6 U1 5 U2 37 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 524 EP 534 DI 10.2134/jeq2014.07.0301 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000022 PM 26023971 ER PT J AU Bjorneberg, DL Leytem, AB Ippolito, JA Koehn, AC AF Bjorneberg, David L. Leytem, April B. Ippolito, James A. Koehn, Anita C. TI Phosphorus Losses from an Irrigated Watershed in the Northwestern United States: Case Study of the Upper Snake Rock Watershed SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID 2 LARGE TRACTS; FURROW EROSION; DRAINAGE WATERS; SOUTHERN IDAHO; RETURN FLOWS; SEDIMENT; RUNOFF; INFILTRATION; CONSERVATION; TRANSPORT AB Watersheds using surface water for irrigation often return a portion of the water to a water body. This irrigation return flow often includes sediment and nutrients that reduce the quality of the receiving water body. Research in the 82,000-ha Upper Snake Rock (USR) watershed from 2005 to 2008 showed that, on average, water diverted from the Snake River annually supplied 547 kg ha(-1) of total suspended solids (TSS), 1.1 kg ha(-1) of total P (TP), and 0.50 kg ha(-1) of dissolved P (DP) to the irrigation tract. Irrigation return flow from the USR watershed contributed 414 kg ha(-1) of TSS, 0.71 kg ha(-1) of TP, and 0.32 kg ha(-1) of DP back to the Snake River. Significantly more TP flowed into the watershed than returned to the Snake River, whereas there was no significant difference between inflow and return flow loads for TSS and DP. Average TSS and TP concentrations in return flow were 71 and 0.12 mg L-1, respectively, which exceeded the TMDL limits of 52 mg L-1 TSS and 0.075 mg L-1 TP set for this section of the Snake River. Monitoring inflow and outflow for five water quality ponds constructed to reduce sediment and P losses from the watershed showed that TSS concentrations were reduced 36 to 75%, but DP concentrations were reduced only 7 to 16%. This research showed that continued implementation of conservation practices should result in irrigation return flow from the USR watershed meeting the total maximum daily load limits for the Snake River. C1 [Bjorneberg, David L.; Leytem, April B.; Ippolito, James A.; Koehn, Anita C.] ARS, USDA, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. RP Bjorneberg, DL (reprint author), ARS, USDA, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM dave.bjorneberg@ars.usda.gov NR 25 TC 4 Z9 4 U1 3 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 552 EP 559 DI 10.2134/jeq2014.04.0166 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000025 PM 26023974 ER PT J AU Han, K Kleinman, PJA Saporito, LS Church, C McGrath, JM Reiter, MS Tingle, SC Allen, AL Wang, LQ Bryant, RB AF Han, Kun Kleinman, Peter J. A. Saporito, Lou S. Church, Clinton McGrath, Joshua M. Reiter, Mark S. Tingle, Shawn C. Allen, Arthur L. Wang, L. Q. Bryant, Ray B. TI Phosphorus and Nitrogen Leaching Before and After Tillage and Urea Application SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID COASTAL-PLAIN SOILS; MANURE APPLICATION; POULTRY LITTER; WATER-QUALITY; NITRATE; LOSSES; SUBSURFACE; TRANSPORT; DYNAMICS; AMMONIUM AB Leaching of nutrients through agricultural soils is a priority water quality concern on the Atlantic Coastal Plain. This study evaluated the effect of tillage and urea application on leaching of phosphorus (P) and nitrogen (N) from soils of the Delmarva Peninsula that had previously been under no-till management. Intact soil columns (30 cm wide x 50 cm deep) were irrigated for 6 wk to establish a baseline of leaching response. After 2 wk of drying, a subset of soil columns was subjected to simulated tillage (0-20 cm) in an attempt to curtail leaching of surface nutrients, especially P. Urea (145 kg N ha(-1)) was then broadcast on all soils (tilled and untilled), and the columns were irrigated for another 8 wk. Comparison of leachate recoveries representing rapid and slow flows confirmed the potential to manipulate flow fractions with tillage, albeit with mixed results across soils. Leachate trends in the finer-textured soil suggest that tillage impeded macropore flow and forced greater matrix flow. Despite significant vertical stratification of soil P that suggested tillage could prevent leaching of P via macropores from the surface to the subsoil, tillage had no significant impact on P leaching losses. Relatively high levels of soil P below 20 cm may have served as the source of P enrichment in leachate waters. However, tillage did lower losses of applied urea in leachate from two of the three soils, partially confirming the study's premise that tillage would destroy macropore pathways transmitting surface constituents to the subsoil. C1 [Kleinman, Peter J. A.; Saporito, Lou S.; Church, Clinton; Bryant, Ray B.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Han, Kun; Wang, L. Q.] Northwest A&F Univ, Coll Resources & Environm, Yangling 712100, Shaanxi, Peoples R China. [McGrath, Joshua M.] Univ Kentucky, Plant & Soil Sci, Lexington, KY 40546 USA. [Reiter, Mark S.] Virginia Tech, Eastern Shore Agr Res & Extens Ctr, Painter, VA 23420 USA. [Tingle, Shawn C.] Univ Delaware, Carvel Res & Educ Ctr, Georgetown, DE 19947 USA. [Allen, Arthur L.] Univ Maryland Eastern Shore, Princess Anne, MD 21853 USA. RP Kleinman, PJA (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM peter.kleinman@ars.usda.gov FU Chinese Service Center for Scholarly Exchange; USDA Agricultural Research Service FX We thank the staff of the USDA-ARS Pasture Systems and Watershed Management Research Laboratory for their contributions to this study. Soil columns were collected by David Otto and Mike Reiner. Laboratory analyses were conducted by Sarah Fishel, Charles Montgomery, and Paul Spock. This project was funded by the Chinese Service Center for Scholarly Exchange and the USDA Agricultural Research Service. NR 44 TC 4 Z9 4 U1 6 U2 47 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 560 EP 571 DI 10.2134/jeq2014.08.0326 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000026 PM 26023975 ER PT J AU Feyereisen, GW Francesconi, W Smith, DR Papiernik, SK Krueger, ES Wente, CD AF Feyereisen, Gary W. Francesconi, Wendy Smith, Douglas R. Papiernik, Sharon K. Krueger, Erik S. Wente, Christopher D. TI Effect of Replacing Surface Inlets with Blind or Gravel Inlets on Sediment and Phosphorus Subsurface Drainage Losses SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID AGRICULTURAL WATERSHEDS; NUTRIENT LOSSES; TILE INLETS; CLAY SOIL; RUNOFF; TRANSPORT; POLLUTION; TILLAGE; QUALITY; SYSTEMS AB Open surface inlets that connect to subsurface tile drainage systems provide a direct pathway for movement of sediment, nutrients, and agrochemicals to surface waters. This study was conducted to determine the reduction in drainage effluent total suspended sediment (TSS) and phosphorus (P) concentrations and loads when open surface inlets were replaced with blind (in gravel capped with 30 cm of soil) or gravel (in very coarse sand/fine gravel) inlets. In Indiana, a pair of closed depressions in adjacent fields was fitted with open inlet tile risers and blind inlets in 2005 and monitored for flow and water chemistry. Paired comparisons on a storm event basis during the growing season for years 2006 to 2013 showed that TSS loads were 40.4 and 14.4 kg ha(-1) event(-1) for tile risers and blind inlets, respectively. Total P (TP) and soluble reactive P (SRP) loads were 66 and 50% less for the blind inlets, respectively. In Minnesota, TSS and SRP concentrations were monitored for 3 yr before and after modification of 24 open inlets to gravel inlets in an unreplicated large-field on-farm study. Median TSS concentrations were 97 and 8.3 mg L-1 and median SRP concentrations were 0.099 and 0.064 mg L-1 for the open inlet and gravel inlet periods, respectively. Median TSS and SRP concentrations were elevated for snowmelt vs. non-snowmelt seasons for open and gravel inlets. Both replacement designs reduced suspended sediment and P concentrations and loads. The Indiana study suggests blind inlets will be effective beyond a 10-yr service life. C1 [Feyereisen, Gary W.] ARS, USDA, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Francesconi, Wendy] Int Ctr Trop Agr CIAT, Lima, Peru. [Smith, Douglas R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Papiernik, Sharon K.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Krueger, Erik S.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Wente, Christopher D.] N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Feyereisen, GW (reprint author), ARS, USDA, Soil & Water Management Res Unit, 439 Borlaug Hall,1991 Upper Buford Cir, St Paul, MN 55108 USA. EM gary.feyereisen@ars.usda.gov NR 40 TC 5 Z9 5 U1 3 U2 19 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 594 EP 604 DI 10.2134/jeq2014.05.0219 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000029 PM 26023978 ER PT J AU Radcliffe, DE Reid, DK Blomback, K Bolster, CH Collick, AS Easton, ZM Francesconi, W Fuka, DR Johnsson, H King, K Larsbo, M Youssef, MA Mulkey, AS Nelson, NO Persson, K Ramirez-Avila, JJ Schmieder, F Smith, DR AF Radcliffe, David E. Reid, D. Keith Blombaeck, Karin Bolster, Carl H. Collick, Amy S. Easton, Zachary M. Francesconi, Wendy Fuka, Daniel R. Johnsson, Holger King, Kevin Larsbo, Mats Youssef, Mohamed A. Mulkey, Alisha S. Nelson, Nathan O. Persson, Kristian Ramirez-Avila, John J. Schmieder, Frank Smith, Douglas R. TI Applicability of Models to Predict Phosphorus Losses in Drained Fields: A Review SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NONPOINT-SOURCE POLLUTION; COASTAL-PLAIN SOILS; SUBSURFACE DRAINAGE; WATER MANAGEMENT; SURFACE RUNOFF; NITROGEN LOSSES; TILE DRAINS; AGRICULTURAL WATERSHEDS; UNCERTAINTY ANALYSIS; TRANSPORT PROCESSES AB Most phosphorus (P) modeling studies of water quality have focused on surface runoff loses. However, a growing number of experimental studies have shown that P losses can occur in drainage water from artificially drained fields. In this review, we assess the applicability of nine models to predict this type of P loss. A model of P movement in artificially drained systems will likely need to account for the partitioning of water and P into runoff, macropore flow, and matrix flow. Within the soil profile, sorption and desorption of dissolved P and filtering of particulate P will be important. Eight models are reviewed (ADAPT, APEX, DRAINMOD, HSPF, HYDRUS, ICECREAMDB, PLEASE, and SWAT) along with P Indexes. Few of the models are designed to address P loss in drainage waters. Although the SWAT model has been used extensively for modeling P loss in runoff and includes tile drain flow, P losses are not simulated in tile drain flow. ADAPT, HSPF, and most P Indexes do not simulate flow to tiles or drains. DRAINMOD simulates drains but does not simulate P. The ICECREAMDB model from Sweden is an exception in that it is designed specifically for P losses in drainage water. This model seems to be a promising, parsimonious approach in simulating critical processes, but it needs to be tested. Field experiments using a nested, paired research design are needed to improve P models for artificially drained fields. Regardless of the model used, it is imperative that uncertainty in model predictions be assessed. C1 [Radcliffe, David E.] Univ Georgia, Crop & Soil Sci Dep, Athens, GA 30602 USA. [Reid, D. Keith] Agr & AgriFood Canada, Guelph, ON N1G 4S9, Canada. [Blombaeck, Karin; Johnsson, Holger; Larsbo, Mats; Persson, Kristian; Schmieder, Frank] Swedish Univ Agr Sci, Dept Soil & Environm, SE-75007 Uppsala, Sweden. [Bolster, Carl H.] ARS, USDA, Food Anim Environm Syst Res Unit, Bowling Green, KY 42104 USA. [Collick, Amy S.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. [Easton, Zachary M.; Fuka, Daniel R.] Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA. [Francesconi, Wendy] Natl Soil Eros Res Lab, W Lafayette, IN USA. [King, Kevin] ARS, USDA, Soil Drainage Res Unit, Columbus, OH USA. [Youssef, Mohamed A.] N Carolina State Univ, Dept Biol & Agr Engn, DS Weaver Lab, Raleigh, NC 27695 USA. [Mulkey, Alisha S.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Nelson, Nathan O.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Ramirez-Avila, John J.] Mississippi State Univ, Civil & Environm Engn Dept, Mississippi State, MS 39762 USA. [Smith, Douglas R.] Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Radcliffe, DE (reprint author), Univ Georgia, Crop & Soil Sci Dep, Athens, GA 30602 USA. EM dradclif@uga.edu NR 153 TC 13 Z9 13 U1 12 U2 56 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 614 EP 628 DI 10.2134/jeq2014.05.0220 PG 15 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000031 PM 26023980 ER PT J AU Adeli, A Sheng, J Jenkins, JN Feng, G AF Adeli, Ardeshir Sheng, J. Jenkins, J. N. Feng, G. TI Composting and Gypsum Amendment of Broiler Litter to Reduce Nutrient Leaching Loss SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID COMBUSTION BY-PRODUCTS; APPLIED POULTRY LITTER; NO-TILL COTTON; FEEDLOT MANURE; CHICKEN LITTER; PHOSPHORUS EXTRACTABILITY; LIVESTOCK FEEDS; SOIL PROPERTIES; RUNOFF QUALITY; SWINE MANURE AB The effect of composted litter relative to fresh litter on leaching losses of nutrients has not been well documented. Fresh and composted broiler litter was surface-applied to bermudagrass (hay) [Cynodon dactylon (L.) Pers.] established in undisturbed soil columns based on N need of the grass in the presence or absence of flue gas desulfurization (FGD) gypsum to evaluate an approach to reduce broiler litter nutrient leaching potential. Columns were periodically leached and biomass was harvested during the 60-d experiment. Total N applied to bermudagrass from broiler litter was 320 kg ha(-1). Gypsum was mixed with fresh and composted litter at the rate based on 20% of litter weight. For composted broiler litter, NO3-N, P, K, Cu, and Zn contents in the leachate obtained from the first leaching event were 58, 50, 40, 32, and 38% less than fresh broiler litter, respectively. Significant decreases in NO3-N (13%), P (53%), Cu (17%), and Zn (28%) in leachate were obtained when gypsum was mixed with fresh broiler litter. Fresh broiler litter and composted broiler litter applications increased bermudagrass growth compared with the control and gypsum significantly increased yields when mixed with broiler litter. Composted broiler litter application significantly increased N and organic C in the soil compared with fresh litter. Results demonstrate that coapplication of composted broiler litter with FGD gypsum provide the most effective management option for minimizing leaching losses of nutrients while sustaining crop productivity. C1 [Adeli, Ardeshir; Jenkins, J. N.; Feng, G.] ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS 39769 USA. [Sheng, J.] Jiangsu Acad Agr Sci, Agr Resources & Environm, Nanjing, Jiangsu, Peoples R China. RP Adeli, A (reprint author), ARS, USDA, Genet & Precis Agr Res Unit, 810 HWY 12 East, Mississippi State, MS 39769 USA. EM ardeshir.adeli@ars.usda.gov NR 60 TC 0 Z9 0 U1 3 U2 26 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 676 EP 683 DI 10.2134/jeq2014.04.0144 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000036 PM 26023985 ER PT J AU Ruan, CQ Hall, DG Liu, B Duan, YP Li, T Hu, HQ Fan, GC AF Ruan, Chuan-Qing Hall, David G. Liu, Bo Duan, Yong-Ping Li, Tao Hu, Han-Qing Fan, Guo-Cheng TI Host-Choice Behavior of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) Under Laboratory Conditions SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Asian citrus psyllid (Diaphorina citri Kuwayama); huanglongbing; host-choice behavior ID GREENING DISEASE; CENTRAL FLORIDA; TRANSMISSION; BIOLOGY; VECTOR AB The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the vector of the bacterial pathogens that cause Huanglongbing (HLB), considered to be the most devastating disease of citrus worldwide. Knowledge of the ACP's host-plant finding behavior aids in our understanding of the epidemiology of HLB and in designing experiments to investigate host plant resistance to ACP. We present the results of research conducted to assess the ability of adult ACP to distinguish between a non-host plant [Rhododendron simsii Planch. (Ericales: Ericaceae)] and two host plant species [Murraya paniculata (L.) Jack and "Lugan" Citrus reticulata Blanco (Sapindales: Rutaceae)] in a caged, free-choice situation. After being introduced into cages with the three plant species, more than 9 h elapsed before adult ACP made definite plant choices. Subsequently for more than 7 days, although adults were observed on R. simsii each time the plants were inspected, greater numbers were usually observed on M. paniculata or C. reticulata. Ultimately, most adults were on C. reticulata, intermediate numbers were on M. paniculata, and few were on R. simsii. However, at least some ACP adults were always observed on R. simsii, suggesting that adults regularly moved within a cage among the plant species. Regular movement of adults between plants would favor dissemination not only of its population but also of HLB. Adult ACP showed a strong preference for settling on flush leaves as opposed to mature leaves or other plant locations, and larger flush shoots were as attractive as tiny new shoots. It is well known that host plant volatiles such as those associated with flush attract ACP adults and regulate how fast and where they settle on a plant. C1 [Ruan, Chuan-Qing; Liu, Bo; Hu, Han-Qing; Fan, Guo-Cheng] Fujian Acad Agr Sci, Fuzhou 350003, Fujian, Peoples R China. [Hall, David G.; Duan, Yong-Ping] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Li, Tao] Fujian Agr & Forestry Univ, Fuzhou 350003, Fujian, Peoples R China. RP Liu, B (reprint author), Fujian Acad Agr Sci, Fuzhou 350003, Fujian, Peoples R China. EM ruanchuanqing@163.com; David.Hall@ars.usda.gov; liubofaas@163.com; YongPing.Duan@ars.usda.gov; 865796158@qq.com; 597550452@qq.com; 717760625@qq.com FU project "Evaluation of citrus germplasm for psyllid resistance in China" of US Department of Agriculture [6618-22000-037-01S]; Provincial Natural Science Foundation of Fujian, China [2012 J01106]; Chinese Special Fund for Agro-scientific Research in the Public Interest [201003067-05] FX The authors thank the financial support of the project "Evaluation of citrus germplasm for psyllid resistance in China" (No: 6618-22000-037-01S) of US Department of Agriculture. This study was also partially funded by Provincial Natural Science Foundation of Fujian, China (2012 J01106) and Chinese Special Fund for Agro-scientific Research in the Public Interest (No. 201003067-05). NR 23 TC 1 Z9 1 U1 3 U2 28 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD MAR PY 2015 VL 28 IS 2 BP 138 EP 146 DI 10.1007/s10905-015-9488-2 PG 9 WC Entomology SC Entomology GA CE0VB UT WOS:000351525100005 ER PT J AU Cooperband, MF Hartness, A Zilkowski, B Lelito, J Cosse, A AF Cooperband, Miriam F. Hartness, Ashley Zilkowski, B. Lelito, Jon Cosse, Allard TI Attraction of Spathius Agrili Yang (Hymenoptera: Eulophidae) to Male-Produced "Aggregation-sex Pheromone": Differences Between the Sexes and Mating Status SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Aggregation-sex pheromone; attraction; parasitoid; physiological state; mating; emerald ash borer ID EMERALD ASH BORER; TETRASTICHUS-PLANIPENNISI HYMENOPTERA; COLEOPTERA BUPRESTIDAE; BRACONIDAE; IDENTIFICATION; CHINA AB Male and female Spathius agrili Yang were tested for attraction to the synthetic male pheromone. Lures consisting of a 3-component pheromone blend were placed in the center of a white filter paper target used to activate upwind flight in the wind tunnel. When virgin males and females were tested for attraction, both sexes were attracted to the lure prior to mating. However, only males were attracted to the pheromone lures after mating. In another experiment, of females that flew to the lure as virgins, half were subsequently mated and the other half were not, and mated females were no longer attracted. Then both mated and virgin females were provided with host material (emerald ash borer larvae in sticks of ash) to determine if oviposition affected attraction. They were supplied with fresh hosts ad libidum and subsequently tested for attraction for 55 days, and results showed that oviposition did not affect attraction to the pheromone. The key factor in attraction to the pheromone was mating. Because this pheromone is released by one sex and is attractive to both sexes for the purpose of mating, it qualifies as an "aggregation-sex pheromone". C1 [Cooperband, Miriam F.; Hartness, Ashley] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Zilkowski, B.; Cosse, Allard] USDA ARS NCAUR, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. [Lelito, Jon] USDA APHIS PPQ Emerald Ash Borer Program, Brighton, MI 48116 USA. RP Cooperband, MF (reprint author), USDA APHIS PPQ CPHST, Otis Lab, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. EM miriam.f.cooperband@aphis.usda.gov NR 33 TC 2 Z9 2 U1 1 U2 7 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD MAR PY 2015 VL 28 IS 2 BP 167 EP 174 DI 10.1007/s10905-015-9492-6 PG 8 WC Entomology SC Entomology GA CE0VB UT WOS:000351525100008 ER PT J AU Cosse, AA Zilkowski, BW AF Cosse, Allard A. Zilkowski, Bruce W. TI Behavioral Responses of Lesser Mealworm Beetles, Alphitobius diaperinus, (Coleoptera: Tenebrionidae) to Pheromone Components Using a Wind Tunnel Dual Choice Walking Bioassay SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Aggregation pheromone; wind tunnel bioassay; Alphitobius diaperinus; lesser mealworm; poultry pest; Tenebrionidae; Coleoptera ID BASE-LINE RESPONSES; AGGREGATION PHEROMONE; RESERVOIR COMPETENCE; SALMONELLA-TYPHIMURIUM; GNATOCERUS-CORNUTUS; ESCHERICHIA-COLI; BROILER HOUSES; DISEASE VIRUS; PANZER; IDENTIFICATION AB The lesser mealworm beetle (LMW), Alphitobius diaperinus (Panzer), is a serious pest in poultry production facilities world-wide and is difficult to control by conventional means. Pheromone-based tools might become useful in the management of this species. Male LMW emit a five-component pheromone consisting of, (R)-(+)-limonene, (E)-beta-ocimene, (S)-(+)-linalool, (R)-(+)-daucene, and 2-nonanone. Three of the five components ((E)-beta-ocimene, (R)-(+)-daucene, and 2-nonanone) had shown behavioral activity, but it was not yet known if different blend combinations of the five components could modulate the beetle behavioral response. We developed a dual choice wind tunnel walking bioassay to test the attraction of male and female adult beetles to individual pheromone components and their blends. We tested hundreds of male and female adults at the same time to create a high throughput bioassay, which confirmed that only three compounds, (E)-beta-ocimene, (R)-(+)-daucene, and 2-nonanone, are necessary to exhibit the pheromone behavioral activity of the naturally emitted blend. Randomly roaming males and females responded by forming lines while walking up to a source of attractive odor. C1 [Cosse, Allard A.; Zilkowski, Bruce W.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Cosse, AA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Allard.Cosse@ars.usda.gov FU cooperative research and development agreement (CRADA); BASF Corporation FX We like to thank Kyle Schmitt, Kelly Buchek, and Savanna Dorsey for their technical assistance. We like to thank Debora Palmquist, USDA-ARS Midwest Area Office, for her help with the statistical analysis, and Miriam Cooperband, USDA-APHIS-CPHST, Otis Laboratory, Buzzard Bay, MA, for critically reading the manuscript and for her helpful suggestions. This investigation has been supported in part by funds made available through a cooperative research and development agreement (CRADA) with the BASF Corporation. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 24 TC 1 Z9 1 U1 5 U2 16 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD MAR PY 2015 VL 28 IS 2 BP 202 EP 210 DI 10.1007/s10905-015-9493-5 PG 9 WC Entomology SC Entomology GA CE0VB UT WOS:000351525100011 ER PT J AU Terrazas-Hernandez, JA Berrios, JD Glenn, GM Imam, SH Wood, D Bello-Perez, LA Vargas-Torres, A AF Terrazas-Hernandez, Jorge A. Berrios, Jose De J. Glenn, Gregory M. Imam, Syed H. Wood, Delilah Bello-Perez, Luis A. Vargas-Torres, Apolonio TI Properties of Cast Films Made of Chayote (Sechium edule Sw.) Tuber Starch Reinforced with Cellulose Nanocrystals SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Chayotextle starch; Cellulose nanocrystals; Mechanical properties; Biodegradation; Cast films ID MECHANICAL-PROPERTIES; THERMOPLASTIC STARCH; PHYSICAL-PROPERTIES; CASSAVA STARCH; NANOCOMPOSITE FILMS; CRYSTALLINITY; CLAY; NANOPARTICLES; NANOFIBRILS; GLYCEROL AB In this study, cellulose (C) and cellulose nanocrystals (CN) were blended with chayotextle starch to prepare films for the casting method. The films were stored at different temperatures and relative humidity (RH), and were analyzed by mechanical tests, X-ray diffraction, thermogravimetric analysis and biodegradation rate. The results of the mechanical characterization showed the highest values in the films with CN. In general, both type of films (with C and CN) presented an increase in the mechanical properties when the RH and temperature increased. The X-ray diffraction pattern of the films did not show any appreciable change from the effect of RH and temperature during storage. The thermogravimetric test showed that the RH and temperature of the storage did not affect the loss mass rate. The biodegradation of the films with C and CN was higher by temperature than RH, but at the longest storage times (up to 20 days) no change was observed by storage condition. C1 [Terrazas-Hernandez, Jorge A.; Vargas-Torres, Apolonio] Univ Autonoma Estado Hidalgo, Inst Ciencias Agr, Ctr Invest Ciencia & Tecnol Alimentos, Rancho Univ, Tulancingo 43600, Hidalgo, Mexico. [Berrios, Jose De J.; Glenn, Gregory M.; Imam, Syed H.; Wood, Delilah; Vargas-Torres, Apolonio] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Bello-Perez, Luis A.] Inst Politecn Nacl, CEPROBI, Yautepec 62731, Morelos, Mexico. RP Vargas-Torres, A (reprint author), Univ Autonoma Estado Hidalgo, Inst Ciencias Agr, Ctr Invest Ciencia & Tecnol Alimentos, Rancho Univ, Av Univ Km 1, Tulancingo 43600, Hidalgo, Mexico. EM apolovt@hotmail.com FU PROMEP-SEP; CONACyT-Mexico [167500] FX We appreciate the financial support from PROMEP-SEP and CONACyT-Mexico (167500). Western Regional Research Center (WRRC) of the United State Department of Agriculture (USDA). NR 47 TC 2 Z9 2 U1 0 U2 12 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 EI 1572-8900 J9 J POLYM ENVIRON JI J. Polym. Environ. PD MAR PY 2015 VL 23 IS 1 BP 30 EP 37 DI 10.1007/s10924-014-0652-0 PG 8 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA CD2FM UT WOS:000350890200004 ER PT J AU Smith, DR King, KW Williams, MR AF Smith, Douglas R. King, Kevin W. Williams, Mark R. TI What is causing the harmful algal blooms in Lake Erie? SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article ID SOIL; CONSEQUENCE; TRIBUTARIES C1 [Smith, Douglas R.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [King, Kevin W.; Williams, Mark R.] USDA ARS, Soil Drainage Res Unit, Columbus, OH USA. RP Smith, DR (reprint author), USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. NR 17 TC 24 Z9 24 U1 11 U2 64 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2015 VL 70 IS 2 BP 27A EP 29A DI 10.2489/jswc.70.2.27A PG 3 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CD2DO UT WOS:000350884500001 ER PT J AU Hyberg, S Iovanna, R Crumpton, W Richmond, S AF Hyberg, Skip Iovanna, Rich Crumpton, William Richmond, Shawn TI The cost effectiveness of wetlands designed and sited for nitrate removal: The effect of increased efficiency, rising easement costs, and lower interest rates SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article C1 [Hyberg, Skip; Iovanna, Rich] USDA, Farm Serv Agcy, Washington, DC 20250 USA. [Crumpton, William] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA USA. [Richmond, Shawn] Iowa Dept Agr & Land Stewardship, Iowa Conservat Reserve Enhancement Program, Des Moines, IA USA. RP Hyberg, S (reprint author), USDA, Farm Serv Agcy, Washington, DC 20250 USA. NR 8 TC 0 Z9 0 U1 1 U2 7 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2015 VL 70 IS 2 BP 30A EP 32A DI 10.2489/jswc.70.2.30A PG 3 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CD2DO UT WOS:000350884500002 ER PT J AU Mwangi, JK Shisanya, CA Gathenya, JM Namirembe, S Moriasi, DN AF Mwangi, J. K. Shisanya, C. A. Gathenya, J. M. Namirembe, S. Moriasi, D. N. TI A modeling approach to evaluate the impact of conservation practices on water and sediment yield in Sasumua Watershed, Kenya SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE calibration; conservation; ecosystems; soil erosion; Soil and Water Assessment Tool (SWAT); validation ID SWAT MODEL; SOIL LOSS; MANAGEMENT-PRACTICES; GRASSED WATERWAY; HYDROLOGIC MODEL; ASSESSMENT-TOOL; FILTER STRIPS; RUNOFF; QUALITY; EROSION AB Degradation of agricultural watersheds often reduces their capacity to provide ecosystem services such as sediment retention, flow regulation, and water quality improvement. Soil and water conservation practices can be used to enhance the capacity of watersheds to produce these services. The objective of this study was to evaluate the impact of agricultural conservation practices on water and sediment yield using the Soil and Water Assessment Tool (SWAT) model. The study area was Sasumua Watershed (107 km(2) [43 mi(2)]), where land degradation has affected watershed's capacity to regulate flow and maintain water quality. The model was calibrated and validated for streamflow at the watershed outlet. Data on annual average erosion rates for the area was used to constrain soil erodibility factor (KUSLE) and practice erodibility factor (PUSLE) parameters during sediment calibration while measured three month sediment concentration data was used for validation. Model performance was assessed using the coefficient of determination (r(2)), Nash-Sutcliffe efficiency coefficient (ENS) and percent bias (PBIAS). Results gave monthly streamflow r(2) values of 0.80 and 0.85, ENS values of 0.74 and 0.81, and PBIAS values of +/- 5% and +/- 6% during the calibration and validation. The model also satisfactorily simulated daily sediment concentrations with an r(2) value of 0.54. However, ENS and PBIAS values were low, which was attributed to the short duration of measurement. The validated model was used to simulate sediment yield for the period 1970 to 2010. Mean annual watershed sediment yield was 40,934 t y(-1) (9 0,243,096 lb yr(-1)). The impacts of filter strips, contour farming, parallel terraces, grassed waterways, and their combinations on water and sediment yield were simulated by adjusting relevant model parameters. The effectiveness of filter strips increased nonlinearly with width being optimum at 30 m (98.4 ft). A combination of 30 m (98.4 ft) wide filter strips and grassed waterways reduced sediment yield by 80%; parallel terraces, 10 m (32.8 ft) filter strips, and grassed waterways reduced sediment yield by 75%; 10 m (32.8 ft) filter strips and grassed waterways reduced yield by 73%; contour farming and grassed waterways reduced yield by 66%; and grassed waterways reduced yield by only 54%. Parallel terraces reduced surface runoff by 20% and increased base flow by 12%, while contour farming reduced surface runoff by 12% and increased base flow by 6.5%. Implementation of conservation practices can reduce sediment yield and increase water yield marginally. The results give an insight into the implications of the present land use management practices and can be used to devise ecologically sound watershed management and development plans. C1 [Mwangi, J. K.; Shisanya, C. A.] Kenyatta Univ, Nairobi, Kenya. [Gathenya, J. M.] Jomo Kenyatta Univ Agr & Technol, Juja, Kenya. [Namirembe, S.] World Agroforestry Ctr, Environm Serv, Nairobi, Kenya. [Moriasi, D. N.] USDA ARS, Grazinglands Res Lab, Washington, DC 20250 USA. RP Mwangi, JK (reprint author), Kenyatta Univ, Nairobi, Kenya. FU Jomo Kenyatta University of Agriculture and Technology; Pro-Poor Rewards for Environmental Services (PRESA) project of the World Agro forestry Centre (ICRAF) at Nairobi, Kenya FX This study was funded by Jomo Kenyatta University of Agriculture and Technology and the Pro-Poor Rewards for Environmental Services (PRESA) project of the World Agro forestry Centre (ICRAF) at Nairobi, Kenya. NR 67 TC 2 Z9 3 U1 1 U2 17 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2015 VL 70 IS 2 BP 75 EP 90 DI 10.2489/jswc.70.2.75 PG 16 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CD2DO UT WOS:000350884500005 ER PT J AU Gautam, S Mbonimpa, EG Kumar, S Bonta, JV Lal, R AF Gautam, S. Mbonimpa, E. G. Kumar, S. Bonta, J. V. Lal, R. TI Agricultural Policy Environmental eXtender model simulation of climate change impacts on runoff from a small no-till watershed SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE Agricultural Policy Environmental eXtender model; climate change; cover crop; management; no-till; runoff ID CORN-SOYBEAN ROTATION; ORGANIC-CARBON; CROP ROTATIONS; COVER CROPS; SOIL; LOSSES; INFILTRATION; HYDROLOGY; EROSION; RESIDUE AB Long-term hydrologic data sets are required to quantify the impacts of management and climate on runoff at the field scale where management practices are applied. This study was conducted to evaluate the impacts of long-term management and climate on runoff from a small watershed managed with no-till (NT) system. The Agricultural Policy Environmental eXtender (APEX), a field scale hydrologic model which is capable of simulating the management and climate impacts on runoff, was used in this study. The specific objectives of the study were to (1) simulate the impacts of cropping management and tillage system on runoff and (2) simulate climate change impacts on runoff using different temperature, precipitation, and carbon dioxide (CO2) scenarios generated from the APEX model. The study was conducted on a small watershed located on the North Appalachian Experimental Watershed (NAEW) near Coshocton, Ohio. This watershed (WS 118, similar to 0.79 ha [1.95 ac]) includes NT management with two periods of crop rotations: corn (Zea mays L.)-soybean (Glycine max L.)-rye (Secale cereale L.) (CSR; 2000 to 2005) and continuous corn (CC; 2006 to 2011). The results from this study indicate that the CSR rotation showed 37% lower simulated mean annual runoff compared with that of CC under NT system. The climate change scenarios indicated runoff was most sensitive to the precipitation, and interactions of precipitation, temperature, and CO2 concentrations. The highest increase of runoff (61%) was observed with 15% increase of precipitation, and the highest reduction in runoff (47%) with 15% decrease in precipitation, demonstrating the nonlinearity of hydrological systems. The results demonstrate the benefits of cover crops in the CSR over the CC rotation under NT system and show the significant impacts of climate change on runoff response from a small, upland, agricultural watershed. For future research, climate change impacts on runoff can be assessed using downscaled climate models that take into consideration interaction among weather parameters. C1 [Gautam, S.; Mbonimpa, E. G.; Kumar, S.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Bonta, J. V.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Lal, R.] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. RP Gautam, S (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. FU USDA National Institute of Food and Agriculture [OHO01089-SS] FX The authors would like to thank the USDA Agricultural Research Service personnel for providing the hydrology data and watershed information for this study. We also thank Robin Taylor, senior research scientist, Blackland Research and Extension Center, Temple, Texas, for his comments related to statistical analysis. Further, this research is part of a project supported by the USDA National Institute of Food and Agriculture, Project No. OHO01089-SS, "Quantifying the spatial location of small-scale land management changes in large watersheds using hydrological modeling." NR 58 TC 2 Z9 2 U1 3 U2 15 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2015 VL 70 IS 2 BP 101 EP 109 DI 10.2489/jswc.70.2.101 PG 9 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CD2DO UT WOS:000350884500007 ER PT J AU Svanback, A Ulen, B Bergstrom, L Kleinman, PJA AF Svanback, A. Ulen, B. Bergstrom, L. Kleinman, P. J. A. TI Long-term trends in phosphorus leaching and changes in soil phosphorus with phytomining SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE high phosphorus soils; phosphorus drawdown; phosphorus leaching; phytomining ID FERTILITY EXPERIMENTS; AGRICULTURAL SOILS; WATER-QUALITY; CLASSIFICATION; GRASSLAND; DRAINAGE; LOSSES; RUNOFF; SITES; SUBSURFACE AB Few mitigation strategies exist to reduce phosphorus (P) losses in leachate once soil P has built up. "Phytomining," or harvesting a crop without application of fertilizer P to create a negative P balance, has been proposed as a strategy for lowering soil P levels and preventing P loss to runoff and leachate. In this study crops were grown and harvested over 7 to 16 years in undisturbed soil columns (105 cm [41 in] deep) with contrasting textures (loamy sand, sandy loam, silty clay loam, and clay) and high P levels, while P loss in leachate was measured. Soil test P in the topsoil (0 to 20 cm [0 to 8 in] depth) was significantly decreased from the beginning to the end of the study for all soils, while a significant decreasing trend in dissolved reactive P in leachate was only observed in one soil. Downward movement of P from the topsoil to deeper layers was indicated to occur in three out of four soils. Although phytomining lowered soil test P by 11% to 37% in topsoils over the 7 to 16 year period of the study, results indicate that soils with P content well above agronomic optimum may take a much longer time to reach the agronomic optimum. C1 [Svanback, A.] Stockholm Univ, Balt Sea Ctr, S-10691 Stockholm, Sweden. [Ulen, B.; Bergstrom, L.] Swedish Univ Agr Sci, Dept Soil & Environm, Uppsala, Sweden. [Kleinman, P. J. A.] USDA ARS, University Pk, PA USA. RP Svanback, A (reprint author), Stockholm Univ, Balt Sea Ctr, S-10691 Stockholm, Sweden. NR 48 TC 1 Z9 1 U1 6 U2 26 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2015 VL 70 IS 2 BP 121 EP 132 DI 10.2489/jswc.70.2.121 PG 12 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CD2DO UT WOS:000350884500009 ER PT J AU Wright, JA Larson, RT Richardson, AG Cote, NM Stoops, CA Clark, M Obenauer, PJ AF Wright, Jennifer A. Larson, Ryan T. Richardson, Alec G. Cote, Noel M. Stoops, Craig A. Clark, Marah Obenauer, Peter J. TI COMPARISON OF BG-SENTINEL (R) TRAP AND OVIPOSITION CUPS FOR AEDES AEGYPTI AND AEDES ALBOPICTUS SURVEILLANCE IN JACKSONVILLE, FLORIDA, USA SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Aedes aegypti; Aedes albopictus; BG-Sentinel; oviposition cup surveillance; Florida; dengue ID HABITAT SEGREGATION; SOUTH FLORIDA; DENGUE VIRUS; TRANSMISSION; MOSQUITOS; CULICIDAE; INVASION; DIPTERA AB The BG-Sentinele (R) (BGS) trap and oviposition cups (OCs) have both proven effective in the surveillance of Aedes species. This study aimed to determine which of the 2 traps could best characterize the relative population sizes of Aedes albopictus and Aedes aegypti in an urban section of Jacksonville, FL. Until 1986, Ae. aegypti was considered the dominant container-breeding species in urban northeastern Florida. Since the introduction of Ae. albopictus, Ae. aegypti has become almost completely extirpated. In 2011, a resurgence of Ae. aegypti was detected in the urban areas of Jacksonville; thus this study initially set out to determine the extent of Ae. aegypti reintroduction to the area. We determined that the BGS captured a greater number of adult Ae. aegypti than Ae. albopictus, while OCs did not monitor significantly different numbers of either species, even in areas where the BGS traps suggested a predominance of one species over the other. Both traps were effective at detecting Aedes spp.; however, the BGS proved more diverse by detecting over 20 other species as well. Our results show that in order to accurately determine vectorborne disease threats and the impact of control operations on these 2 species, multiple trapping techniques should be utilized when studying Ae. aegypti and Ae. albopictus population dynamics. C1 [Wright, Jennifer A.; Larson, Ryan T.; Richardson, Alec G.; Cote, Noel M.; Obenauer, Peter J.] Navy Entomol Ctr Excellence, Naval Air Stn Jacksonville, Jacksonville, FL 32212 USA. [Stoops, Craig A.] USDA ARS, Navy & Marine Corps Publ Hlth Ctr, Ctr Med Agr & Vet Entomol, Gainesville, FL 32068 USA. [Clark, Marah] Jacksonville Mosquito Control Div, Jacksonville, FL 32218 USA. RP Wright, JA (reprint author), Navy Entomol Ctr Excellence, Naval Air Stn Jacksonville, Box 43 Bldg 927, Jacksonville, FL 32212 USA. FU Deployed War Fighter Program FX We thank Jason Francona, Michael Denson, Dante Benedicto, Gregory Allen, and Marcus McDonough for assistance with field collections. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the US government. This work was funded by the Deployed War Fighter Program. The author and coauthors are employees of the US government. This work was prepared as part of our official duties. Title 17 U.S.C. section 17 provides that, "Copyright protection under this title is not available for any work of the United States government." Title 17 U.S.C. section 101 defines a US government work as a work prepared by a military service member or employee of the US government as part of that person's official duties. NR 23 TC 2 Z9 2 U1 1 U2 7 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD MAR PY 2015 VL 31 IS 1 BP 26 EP 31 PG 6 WC Entomology SC Entomology GA CD8DZ UT WOS:000351326700004 PM 25843173 ER PT J AU Bernier, UR Kline, DL Allan, SA Barnard, DR AF Bernier, Ulrich R. Kline, Daniel L. Allan, Sandra A. Barnard, Donald R. TI LABORATORY STUDIES OF AEDES AEGYPTI ATTRACTION TO KETONES, SULFIDES, AND PRIMARY CHLOROALKANES TESTED ALONE AND IN COMBINATION WITH L-LACTIC ACID SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE mosquito attractments; lures; blends; olfactometer ID VOLATILE ORGANIC-COMPOUNDS; CHROMATOGRAPHY-MASS-SPECTROMETRY; YELLOW-FEVER MOSQUITO; HUMAN SKIN EMANATIONS; CARBON-DIOXIDE; CANDIDATE ATTRACTANTS; DIPTERA-CULICIDAE; ANOPHELES-GAMBIAE; MALARIA MOSQUITO; HOST-SEEKING AB The attraction of female Aedes aegypti to single compounds and binary compositions containing L-lactic acid and an additional saturated compound from a set of ketones, sulfides, and chloroalkanes was studied using a triple-cage dual-port olfactometer. These chemical classes were studied because of their structural relation to acetone, dimethyl disulfide, and dichloromethane, which have all been reported to synergize attraction to L-lactic acid. Human odors, carbon dioxide, and the binary mixture of L-lactic acid and CO2 served as controls for comparison of attraction responses produced by the binary inixtures. All tested mixtures that contained chloroalkanes attracted mosquitoes at synergistic levels, as did L-lactic acid and CO2. Synergism was less frequent in mixtures of L-lactic acid with sulfides and ketones; in the case of ketones, synergistic attraction was observed only for L-lactic acid combined with acetone or butanone. Suppression or inhibition of attraction response was observed for combinations that contained ketones of C-7-C-12 molecular chain length (optimum in the C-8-C-10 range). This inhibition effect is similar to that observed previously for specific ranges of carboxylic acids, aldehydes, and alcohols. C1 [Bernier, Ulrich R.; Kline, Daniel L.; Allan, Sandra A.; Barnard, Donald R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Bernier, UR (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. NR 64 TC 0 Z9 0 U1 2 U2 10 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD MAR PY 2015 VL 31 IS 1 BP 63 EP 70 PG 8 WC Entomology SC Entomology GA CD8DZ UT WOS:000351326700008 PM 25843177 ER PT J AU Xue, RD Smith, ML Yi, H Kline, DL AF Xue, Rui-De Smith, Michael L. Yi, Hoonbook Kline, Daniel L. TI FIELD EVALUATION OF A NOVEL MOS-HOLE TRAP AND NAPHTHA COMPARED WITH BG SENTINEL TRAP AND MOSQUITO MAGNET X TRAP TO COLLECT ADULT MOSQUITOES SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Aedes albopictus; Psorophora columbiae; Mos-Hole trap; BG Sentinel trap; Mosquito Magnet X trap; naphtha ID AEDES-ALBOPICTUS; SURVEILLANCE; EFFICACY AB The novel Mos-Hole trap (TM) with liquid attractant naphtha (TM) from Korea was compared with BG Sentinel (TM) trap and Mosquito Magnet X (TM) trap for field collection of adult mosquitoes in St. Johns County, northeastern Florida, from May to October 2013. The novel Mos-Hole trap baited with naphtha (liquid attractant) collected similar numbers of mosquitoes, compared with the number of mosquitoes caught by BG Sentinel traps baited with BG Lure (TM). Both Mos-Hole and BG Sentinel traps collected a significantly greater number of mosquitoes compared with the numbers collected by Mosquito Magnet X traps. In other field evaluations when switching lures, the Mos-Hole traps baited with BG Lure caught more mosquitoes than the BG Sentinel trap baited with liquid naphtha attractant. The results showed that the novel Mos-Hole trap has the potential to be used as an additional effective sampling tool for population surveillance and control of adult mosquitoes. C1 [Xue, Rui-De; Smith, Michael L.] Anastasia Mosquito Control Dist, St Augustine, FL 32080 USA. [Yi, Hoonbook] Seoul Womens Univ, Seoul 139774, South Korea. [Kline, Daniel L.] USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Xue, RD (reprint author), Anastasia Mosquito Control Dist, 500 Old Beach Rd, St Augustine, FL 32080 USA. NR 8 TC 1 Z9 1 U1 0 U2 3 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD MAR PY 2015 VL 31 IS 1 BP 110 EP 112 PG 3 WC Entomology SC Entomology GA CD8DZ UT WOS:000351326700017 PM 25843186 ER PT J AU Allen, AJ Stanton, JB Evermann, JF Fry, LM Ackerman, MG Barrington, GM AF Allen, Andrew J. Stanton, James B. Evermann, James F. Fry, Lindsay M. Ackerman, Melissa G. Barrington, George M. TI Bluetongue disease and seroprevalence in South American camelids from the northwestern region of the United States SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Alpacas; Bluetongue virus; camelids; dyspnea; llamas; pulmonary edema ID VIRUS SEROTYPE 8; EXPERIMENTAL-INFECTION; MEDITERRANEAN BASIN; ALPACA AB In late summer/early fall of 2013, 2 South American camelids from central Washington were diagnosed with fatal bluetongue viral disease, an event which is rarely reported. A 9-year-old intact male llama (Lama glama), with a 1-day history of anorexia, recumbency, and dyspnea before death. Abundant foam discharged from the mouth and nostrils, and the lungs were severely edematous on postmortem examination. Histologically, there was abundant intra-alveolar edema with fibrin. Hemorrhage and edema disrupted several other organs. Bluetongue viral RNA was detected by reverse transcription polymerase chain reaction (RT-PCR), and serotype 11 was identified by sequencing a segment of the VP2 outer capsid gene. Approximately 1 month later, at a site 150 miles north of the index case, a 2-year-old female alpaca with similar, acutely progressive clinical signs was reported. A postmortem examination was performed, and histologic lesions from the alpaca were similar to those of the llama, and again serotype 11 was detected by PCR. The occurrence of bluetongue viral infection and disease is described in the context of seasonal Bluetongue virus activity within the northwestern United States and southwestern Canada. C1 [Allen, Andrew J.; Evermann, James F.; Ackerman, Melissa G.; Barrington, George M.] Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA. [Stanton, James B.; Fry, Lindsay M.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Stanton, James B.; Evermann, James F.; Fry, Lindsay M.] Washington State Univ, Washington Anim Dis Diagnost Lab, Pullman, WA 99164 USA. [Fry, Lindsay M.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. RP Allen, AJ (reprint author), Washington State Univ, Dept Vet Clin Sci, POB 647060, Pullman, WA 99164 USA. EM aja@vetmed.wsu.edu RI Stanton, James/A-5277-2011 OI Stanton, James/0000-0002-7661-2631 NR 26 TC 0 Z9 0 U1 2 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD MAR PY 2015 VL 27 IS 2 BP 226 EP 230 DI 10.1177/1040638715571627 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CE1RT UT WOS:000351590300015 PM 25680921 ER PT J AU Berentsen, AR Vogt, S Guzman, AN Vice, DS Pitt, WC Shiels, AB Spraker, TR AF Berentsen, Are R. Vogt, Scott Guzman, Antenor N. Vice, Daniel S. Pitt, William C. Shiels, Aaron B. Spraker, Terry R. TI Capillaria hepatica infection in black rats (Rattus rattus) on Diego Garcia, British Indian Ocean Territory SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Black rats; Capillaria hepatica; Diego Garcia; Rattus rattus; zoonotic parasite ID NORVEGICUS AB Rats (Rattus spp.) are among the most damaging invasive species worldwide. The accidental introduction of rats has caused significant detriment to native flora and fauna, crops, structures, and human livelihoods. Rats are vectors of disease and carriers of various zoonotic parasites. Capillaria hepatica (syn. Callodium hepaticum) is a parasitic nematode found primarily in rodents but is known to infect over 140 mammal species, including human beings and several species of domestic animals. In this case study, the presence of C. hepatica infection in black rats on Diego Garcia, British Indian Ocean Territory, is reported. Liver samples from 20 black rats (Rattus rattus) were collected during a concurrent population density estimation study. Histology revealed 15 (75%) of the rats sampled had a current or previous infection with C. hepatica. In addition, a larval cestode compatible in size and shape with Cysticercus fasciolaris, the larval stage of Taenia taeniaeformis of cats, was found in 3 (15%) of the rats sampled. The high prevalence of C. hepatica infection in rats on Diego Garcia has implications for human health given the high population density of rats found on the island. C1 [Berentsen, Are R.] WS, USDA, APHIS, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Vogt, Scott] Naval Facil Engn Command, Yokosuka, Kanagawa, Japan. [Guzman, Antenor N.] Naval Facil Engn Command, Diego Garcia, British Indian, England. [Vice, Daniel S.] WS, USDA, APHIS, Hawaii Guam Pacific Isl Off, Barrigada, GU USA. [Pitt, William C.; Shiels, Aaron B.] WS, USDA, APHIS, Natl Wildlife Res Ctr,Hawaii Field Stn, Hilo, HI USA. [Spraker, Terry R.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. RP Berentsen, AR (reprint author), Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM are.r.berentsen@aphis.usda.gov FU U.S. Department of Defense [13-73-15-6576-IA] FX This research was funded through U.S. Department of Defense Agreement number 13-73-15-6576-IA. NR 18 TC 0 Z9 0 U1 3 U2 6 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD MAR PY 2015 VL 27 IS 2 BP 241 EP 244 DI 10.1177/1040638715573298 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA CE1RT UT WOS:000351590300018 PM 25691507 ER PT J AU Brown, RH Singh, J Singh, S Dahleen, LS Lemaux, PG Stein, N Mascher, M Bregitzer, P AF Brown, Ryan H. Singh, Jaswinder Singh, Surinder Dahleen, Lynn S. Lemaux, Peggy G. Stein, Nils Mascher, Martin Bregitzer, Phil TI Behavior of a modified Dissociation element in barley: a tool for genetic studies and for breeding transgenic barley SO MOLECULAR BREEDING LA English DT Article DE Dissociation; Transgene delivery; Transposon tagging; Barley ID AGROBACTERIUM-MEDIATED TRANSFORMATION; TUMEFACIENS C58 DERIVATIVES; T-DNA; INSERTIONAL MUTAGENESIS; EXPRESSION STABILITY; PARTICLE BOMBARDMENT; TRANSPOSON EXCISION; EFFICIENT METHOD; LAUNCH PADS; PLANTS AB Maize-derived sequences from the transposable elements Activator (Ac) and Dissociation (Ds) have enabled studies of gene function via transposon tagging. The characteristics of modified, transgene-containing Ds elements constructed for some of these studies have demonstrated their ability to resolve complex loci, separate transgenes from marker genes and vector sequences, and to support high and heritable levels of transgene expression. To most efficiently design breeding schemes for developing transgenic populations via Ds-mediated transposition, detailed knowledge of the dynamics and characteristics of transposition in barley is necessary. Examination of a barley transposon tagging population (n = 4,954) derived from crosses of lines containing Ds-bar insertions to lines expressing Ac transposase showed that the frequencies of transposition from eight original Ds-bar loci ranged from 5 to 41 % among F-2 individuals. Sequence analysis of Ds-bar terminal sequences and of flanking genomic sequences for 107 F-2 and F-3 individuals indicated precise integrations. Analysis of 173 flanking sequences derived from these populations and from previously produced populations, primarily using sequence-based methods, enabled the mapping of 159 to a specific chromosome and 136 to specific map locations. Of the 156 DsT loci that could be located to specific contigs, most were located in gene-rich areas and approximately 40 % were either in or near (within 1 kb) expressed sequences or predicted proteins. These data will enable the design of optimal breeding schemes for developing and using Ds-based systems for transposon tagging and for transgene delivery that are specific to barley. C1 [Brown, Ryan H.; Bregitzer, Phil] USDA ARS, Natl Small Grains Res Facil, Aberdeen, ID 83210 USA. [Singh, Jaswinder; Singh, Surinder] McGill Univ, Dept Plant Sci, Montreal, PQ H9X 3V9, Canada. [Dahleen, Lynn S.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Lemaux, Peggy G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Stein, Nils; Mascher, Martin] Leibniz Inst Plant Genet & Crop Plant Res IPK, D-06466 Seeland, Germany. RP Bregitzer, P (reprint author), USDA ARS, Natl Small Grains Res Facil, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM phil.bregitzer@ars.usda.gov FU Agricultural Research Service, USDA [5366-21000-031-00D, 5442-21000-036-00D] FX Funding for this research was provided by the Agricultural Research Service, USDA, projects 5366-21000-031-00D and 5442-21000-036-00D. The USDA-ARS is an equal opportunity employer. NR 54 TC 2 Z9 2 U1 0 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2015 VL 35 IS 3 AR 85 DI 10.1007/s11032-015-0193-9 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CD8AM UT WOS:000351317500003 ER PT J AU Myles, S Mahanil, S Harriman, J Gardner, KM Franklin, JL Reisch, BI Ramming, DW Owens, CL Li, L Buckler, ES Cadle-Davidson, L AF Myles, Sean Mahanil, Siraprapa Harriman, James Gardner, Kyle M. Franklin, Jeffrey L. Reisch, Bruce I. Ramming, David W. Owens, Christopher L. Li, Lin Buckler, Edward S. Cadle-Davidson, Lance TI Genetic mapping in grapevine using SNP microarray intensity values SO MOLECULAR BREEDING LA English DT Article DE Vitis; Grapevine; SNP discovery; Genotyping microarray ID GENOME-WIDE ASSOCIATION; GENOTYPE CALLING ALGORITHM; VITIS; TECHNOLOGY; TRAITS; ASSAY; SEX; RESISTANCE; SELECTION; SEQUENCE AB Genotyping microarrays are widely used for genetic mapping, but in high-diversity organisms, the quality of SNP calls can be diminished by genetic variation near the assayed nucleotide. To address this limitation in grapevine, we developed a simple heuristic that uses hybridization intensity to genetically map phenotypes without the need to distinguish between polymorphic states. We applied this approach to the mapping of three previously mapped traits, each controlled by single major effect loci-color, flower sex, and powdery mildew resistance-and confirmed that intensity values outperform SNP calls in all cases. Further, because per sample cost is a major limitation to the adoption of genotyping microarrays in applied genetic research and plant breeding, we tested how many samples were required to map a Mendelian trait in an F1 grape population and found that we could identify the correct genomic region with as few as 12 samples. For high-diversity species for which genotyping arrays are available or under development, our findings suggest a powerful and cost-effective approach to identify large-effect QTL when faced with poor SNP quality. C1 [Myles, Sean; Gardner, Kyle M.] Dalhousie Univ, Fac Agr, Dept Plant & Anim Sci, Truro, NS B2N 5E3, Canada. [Mahanil, Siraprapa; Harriman, James; Owens, Christopher L.; Cadle-Davidson, Lance] ARS, USDA, Grape Genet Res Unit, Geneva, NY USA. [Harriman, James; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, USDA, ARS, Ithaca, NY USA. [Franklin, Jeffrey L.] Agr & Agri Food Canada, Kentville, NS, Canada. [Reisch, Bruce I.] Cornell Univ, New York State Agr Expt Stn, Dept Hort, Geneva, NY 14456 USA. [Ramming, David W.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. [Li, Lin] Harvard Univ, Dept Biostat, Boston, MA 02115 USA. RP Myles, S (reprint author), Dalhousie Univ, Fac Agr, Dept Plant & Anim Sci, Truro, NS B2N 5E3, Canada. EM sean.myles@dal.ca; lance.cadledavidson@ars.usda.gov FU USDA-Agricultural Research Service; National Science and Engineering Research Council (NSERC) of Canada; Canada Research Chairs program FX We thank Jeremiah Degenhardt and Carlos D. Bustamante for useful discussion. This research was undertaken thanks to funding from the USDA-Agricultural Research Service, the National Science and Engineering Research Council (NSERC) of Canada and the Canada Research Chairs program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 4 Z9 4 U1 9 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2015 VL 35 IS 3 AR 88 DI 10.1007/s11032-015-0288-3 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CD8AM UT WOS:000351317500006 ER PT J AU Wu, YK Molongoski, JJ Winograd, DF Bogdanowicz, SM Louyakis, AS Lance, DR Mastro, VC Harrison, RG AF Wu, Yunke Molongoski, John J. Winograd, Deborah F. Bogdanowicz, Steven M. Louyakis, Artemis S. Lance, David R. Mastro, Victor C. Harrison, Richard G. TI Genetic structure, admixture and invasion success in a Holarctic defoliator, the gypsy moth (Lymantria dispar, Lepidoptera: Erebidae) SO MOLECULAR ECOLOGY LA English DT Article DE global phylogeography; hybridization; invasion success; microsatellite; mitochondrial DNA; Pleistocene glaciation ID POPULATION-STRUCTURE; PHYLOGENETIC NETWORKS; RANGE EXPANSION; DNA VARIATION; ICE AGES; SPECIATION; DIVERSITY; EVOLUTION; DISTANCE; PHYLOGEOGRAPHY AB Characterizing the current population structure of potentially invasive species provides a critical context for identifying source populations and for understanding why invasions are successful. Non-native populations inevitably lose genetic diversity during initial colonization events, but subsequent admixture among independently introduced lineages may increase both genetic variation and adaptive potential. Here we characterize the population structure of the gypsy moth (Lymantria dispar Linnaeus), one of the world's most destructive forest pests. Native to Eurasia and recently introduced to North America, the current distribution of gypsy moth includes forests throughout the temperate region of the northern hemisphere. Analyses of microsatellite loci and mitochondrial DNA sequences for 1738 individuals identified four genetic clusters within L.dispar. Three of these clusters correspond to the three named subspecies; North American populations represent a distinct fourth cluster, presumably a consequence of the population bottleneck and allele frequency change that accompanied introduction. We find no evidence that admixture has been an important catalyst of the successful invasion and range expansion in North America. However, we do find evidence of ongoing hybridization between subspecies and increased genetic variation in gypsy moth populations from Eastern Asia, populations that now pose a threat of further human-mediated introductions. Finally, we show that current patterns of variation can be explained in terms of climate and habitat changes during the Pleistocene, a time when temperate forests expanded and contracted. Deeply diverged matrilines in Europe imply that gypsy moths have been there for a long time and are not recent arrivals from Asia. C1 [Wu, Yunke; Molongoski, John J.; Winograd, Deborah F.; Lance, David R.; Mastro, Victor C.] USDA, Otis CPHST Lab, Joint Base Cape Cod, Buzzards Bay, MA 02542 USA. [Wu, Yunke; Bogdanowicz, Steven M.; Harrison, Richard G.] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA. [Louyakis, Artemis S.] Univ Florida, Dept Microbiol & Cell Sci, Kennedy Space Ctr, Space Life Sci Lab, Gainesville, FL 32611 USA. RP Harrison, RG (reprint author), Cornell Univ, Dept Ecol & Evolutionary Biol, Corson Hall, Ithaca, NY 14853 USA. EM rgh4@cornell.edu NR 74 TC 3 Z9 3 U1 14 U2 61 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD MAR PY 2015 VL 24 IS 6 BP 1275 EP 1291 DI 10.1111/mec.13103 PG 17 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CE0AY UT WOS:000351465300010 PM 25655667 ER PT J AU Ikeda, H Callaham, MA O'Brien, JJ Hornsby, BS Wenk, ES AF Ikeda, Hiroshi Callaham, Mac A., Jr. O'Brien, Joseph J. Hornsby, Benjamin S. Wenk, Evelyn S. TI Can the invasive earthworm, Amynthas agrestis, be controlled with prescribed fire? SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Carbon content; Forest management; Nitrogen content; delta C-13; delta N-15 ID TALLGRASS PRAIRIE SOILS; NORTH-AMERICAN FORESTS; SUGARCANE ECOSYSTEM; NATIVE EARTHWORMS; FEEDING ECOLOGY; ORGANIC-MATTER; OLIGOCHAETA; MEGASCOLECIDAE; DIPLOCARDIA; DELTA-N-15 AB Biological invasions are one of the most significant global-scale problems caused by human activities. Earthworms function as ecosystem engineers in soil ecosystems because their feeding and burrowing activities fundamentally change the physical and biological characteristics of the soils they inhabit. As a result of this "engineering," earthworm invasions can have significant effects on soil physical, chemical and biological properties. The species Amynthas agrestis (family Megascolecidae) was introduced to the United States from Asia, and has expanded its distribution range to include relatively undisturbed forests. Here, to clarify life history traits, we reared individuals under seven different conditions of food provision using litter, fragmented litter and soil, and also analyzed the stable isotope ratios of field-collected specimens to investigate their food resources in the field. Second, we examined whether prescribed fire can be used to manage invasive earthworms. We constructed eight experimental plots, each with 100 individuals of A. agrestis each, and burned half of the plots. The feeding experiment showed that the earthworms in units containing soil and some form of organic matter (litter and/or fragmented litter) produced many cocoons, indicating that litter and fragmented litter are important food resources for them. Stable isotope analyses also supported this result. During the experimental fires, average soil temperature at 5 cm depth increased by only 7.7 degrees C (average maximum of 32.2 degrees C). Litter mass was significantly reduced by the fires. Although numbers of A. agrestis and cocoons recovered from burned and unburned plots were not different, the viability of cocoons was significantly lower in burned plots. Fire may also reduce the survival rate of juveniles in the next year by depriving them of their preferred food resource. Most native earthworms in the United States live in the soil, while many invasive ones live in the litter layer and soil surface. Therefore, prescribed fire could be a viable tool for control of invasive earthworms without negatively impacting native earthworm populations. (c) 2014 Elsevier Ltd. All rights reserved. C1 [Ikeda, Hiroshi] Hirosaki Univ, Fac Agr & Life Sci, Hirosaki, Aomori 0368561, Japan. [Callaham, Mac A., Jr.; O'Brien, Joseph J.; Hornsby, Benjamin S.; Wenk, Evelyn S.] US Forest Serv, Ctr Forest Disturbance Sci, So Res Stn, USDA, Athens, GA USA. RP Ikeda, H (reprint author), Hirosaki Univ, Fac Agr & Life Sci, 3 Bunkyo Cho, Hirosaki, Aomori 0368561, Japan. EM hikeda@cc.hirosaki-u.ac.jp RI Ikeda, Hiroshi/B-3940-2011 FU JSPS [22-6882] FX We thank M. Brod for facilitating our work at Chattahoochee National Forest, C.M. Stegall for helping to construct experimental beds, B. Sheko for measuring C and N contents, D. Straube for providing important information, and K. Fukumori for advice on some analyses. We also thank L.G. Callaham and M.M. Callaham for assisting with introduction of earthworms into experimental beds. This work was partially supported by Grant-in-Aid for JSPS Fellows (22-6882). Materials from the GSMNP were collected under permit number GRSM-2011-SCI-0029. NR 42 TC 3 Z9 3 U1 12 U2 40 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD MAR PY 2015 VL 82 BP 21 EP 27 DI 10.1016/j.soilbio.2014.12.011 PG 7 WC Soil Science SC Agriculture GA CD8BW UT WOS:000351321200003 ER PT J AU Lin, H Drohan, P Green, TR AF Lin, Henry Drohan, Patrick Green, Timothy R. TI Hydropedology: The Last Decade and the Next Decade SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Editorial Material ID ISSUES C1 [Lin, Henry; Drohan, Patrick] Penn State Univ, Dep Ecosyst Sci & Management, University Pk, PA 16802 USA. [Green, Timothy R.] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. RP Lin, H (reprint author), Penn State Univ, Dep Ecosyst Sci & Management, University Pk, PA 16802 USA. EM henrylin@psu.edu RI Lin, Henry/E-8234-2011 NR 11 TC 1 Z9 3 U1 4 U2 30 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 357 EP 361 DI 10.2136/sssaj2015.02.0069 PG 5 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900001 ER PT J AU D'Amore, DV Edwards, RT Herendeen, PA Hood, E Fellman, JB AF D'Amore, David V. Edwards, Rick T. Herendeen, Paul A. Hood, Eran Fellman, Jason B. TI Dissolved Organic Carbon Fluxes from Hydropedologic Units in Alaskan Coastal Temperate Rainforest Watersheds SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article DE DOC, dissolved organic carbon; PCTR, perhumid coastal temperate rainforest ID ACROTELM-CATOTELM MODEL; SOUTHEAST ALASKA; HEADWATER CATCHMENT; RUNOFF GENERATION; NORTH-AMERICA; DOC FLUX; EXPORT; NITROGEN; WETLAND; STREAMS AB Dissolved organic C (DOC) transfer from the landscape to coastal margins is a key component of regional C cycles. Hydropedology provides a conceptual and observational framework for linking soil hydrologic function to landscape C cycling. We used hydropedology to quantify the export of DOC from the terrestrial landscape and understand how soil temperature and water table fluctuations regulate DOC losses in the C-rich, perhumid coastal temperate rainforest (PCTR) of Alaska. Land cover in the region is dominated by three major hydropedologic units: poor fen, forested wetland, and upland. We instrumented soils and streams in nine hydropedologic units to quantify DOC fluxes. Stream-water DOC concentrations varied from 5.7 to 16.7 mg C L-1. Mean area-weighted DOC fluxes were 24.8, 29.9, and 10.5 g C m(-2) yr(-1) from the poor fens, forested wetlands, and uplands, respectively. We found that increased soil temperature and frequent fluctuations of soil water tables promoted the export of large quantities of DOC from poor fen and forested wetland units and relatively high amounts of DOC from upland units. The DOC export from the hydropedologic units in the PCTR is among the highest in the world and highlights the importance of terrestrial to aquatic fluxes of DOC as a pathway for C loss in the region. C1 [D'Amore, David V.; Edwards, Rick T.] USDA, Forest Serv, Pacific NW Res Stn, Juneau, AK 99801 USA. [Herendeen, Paul A.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Hood, Eran; Fellman, Jason B.] Univ Alaska Southeast, Environm Sci Program, Juneau, AK 99801 USA. RP D'Amore, DV (reprint author), USDA, Forest Serv, Pacific NW Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. EM ddamore@fs.fed.us FU USDA National Research Initiative [2005-35102-16289] FX We would like to thank Erik Norberg, Jacob Berkowitz, Nick Bonzey, Mark Lukey, and Adelaide Johnson for field and laboratory assistance. This research was partially funded by the USDA National Research Initiative, Grant no. 2005-35102-16289. NR 60 TC 1 Z9 2 U1 3 U2 14 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 378 EP 388 DI 10.2136/sssaj2014.09.0380 PG 11 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900003 ER PT J AU Sherrod, LA Erskine, RH Green, TR AF Sherrod, Lucretia A. Erskine, Robert H. Green, Timothy R. TI Spatial Patterns and Cross-Correlations of Temporal Changes in Soil Carbonates and Surface Elevation in a Winter Wheat-Fallow Cropping System SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID CHEMICAL-PROPERTIES; EROSION; DEPOSITION; COLORADO; TILLAGE; TERRAIN AB Soil erosion and deposition impact the sustainability of agricultural lands within the semiarid Great Plains in the United States. Temporal differences between high-resolution digital elevation maps provide physical estimates of spatial erosion or deposition, and the depth to a calcic horizon is a chemical indicator. We hypothesized that soil surface layer CaCO3 concentration is inversely correlated with the change in surface elevation (Delta z). We studied a 109-ha field in northeastern Colorado under winter wheat (Triticum aestivum L.)-fallow rotation in alternating strips perpendicular to the prevailing wind. Soil samples (top 30 cm) collected from 185 landscape positions in 2001 and 2012 were analyzed for CaCO3 using a modified pressure-calcimeter method. The change in CaCO3 (Delta C) was significantly correlated with large-scale erosional and depositional areas (west and east blocks, respectively) and with soil units, whereas Delta z was correlated with management strips and blocks. The west block had an average Delta C of 3.2 g kg(-1) with 2.0 cm of erosion, whereas the east block decreased by 4.4 g kg(-1) with 4.2 cm of deposition. Summit positions had the highest CaCO3, and toeslope positions had the lowest. We found inverse relationships between Delta z and Delta C in summit and toeslope positions at both erosional (Delta z < -5 cm) and depositional (Delta z > 5 cm) areas, but Delta z was not correlated significantly with Delta C overall. High values of CaCO3 (>100 g kg(-1)) decreased with time. A high-resolution map of Delta z showed complex spatial patterns across scales, which inferred water and wind erosion and deposition affected by terrain and management. C1 [Sherrod, Lucretia A.; Erskine, Robert H.; Green, Timothy R.] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80525 USA. RP Green, TR (reprint author), USDA ARS, Agr Syst Res Unit, 2150-D Ctr Ave 200, Ft Collins, CO 80525 USA. EM tim.green@ars.usda.gov NR 24 TC 0 Z9 0 U1 2 U2 19 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 417 EP 427 DI 10.2136/sssaj2014.05.0222 PG 11 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900007 ER PT J AU Bourgault, RR Ross, DS Bailey, SW AF Bourgault, Rebecca R. Ross, Donald S. Bailey, Scott W. TI Chemical and Morphological Distinctions between Vertical and Lateral Podzolization at Hubbard Brook SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID DEPOSITION; LANDSCAPE; ECOSYSTEM; DYNAMICS; SPODOSOL; ALUMINUM; MATTER AB Classical podzolization studies assumed vertical percolation and pedon-scale horizon development. However, hillslope-scale lateral podzolization also occurs where lateral subsurface water flux predominates. In this hydropedologic study, 99 podzols were observed in Watershed 3, Hubbard Brook Experimental Forest, New Hampshire. Soil horizon samples were extracted with citrate-dithionite (d) and acid ammonium oxalate (o) to quantify Fed, Mnd, Alo, and Feo. Optical density of oxalate extract (ODOE) was measured to assess spodic C. Amorphous organometallic complexes (AOC) were observed in thin section, for which Al, Fe, Mn, and C were quantified using scanning electron microscopy-energy dispersive X-ray spectroscopy. Porosity and AOC/mineral ratio were calculated for thin section images using ImageJ. Laterally developed spodic horizons were twice as thick as vertically developed spodic horizons and contained higher concentrations of Al and Mn but lower Fe and C. Vertically developed spodic horizons had crumb microstructure with higher porosity, while laterally developed spodic horizons were more infilled. Aluminum + 0.5Fe and ODOE in the surface of laterally developed podzols were high and lacked contrast with the spodic horizon, making Spodosol classification problematic. Vertically developed spodic horizons form by solutional translocation and precipitation of AOC under unsaturated conditions. However, laterally developed spodic horizons could form via lateral translocation of solutes or physical transport and deposition of colloidal AOC with unsaturated or saturated flow. This study demonstrates the importance of lateral podzolization in producing soils with distinctive morphology, composition, and classification. Future studies or mapping efforts in podzolized catchments should incorporate these different pedogenic processes. C1 [Bourgault, Rebecca R.; Ross, Donald S.] Univ Vermont, Dept Plant & Soil Sci, Burlington, VT 05405 USA. [Bailey, Scott W.] US Forest Serv, North Woodstock, NH 03262 USA. RP Bourgault, RR (reprint author), Sacred Heart Univ, Dept Biol, 5151 Pk Ave, Fairfield, CT 06825 USA. EM bourgaultr@sacredheart.edu RI Ross, Donald/A-4477-2008; OI Ross, Donald/0000-0002-5390-6602; Bailey, Scott/0000-0002-9160-156X FU NSF EAR Hydrologic Sciences [101450] FX We wish to thank colleagues Kevin McGuire, J.P. Gannon (Virginia Tech) and Tom Bullen (USGS) for collaborative ideas; Michele Von Turkovich (UVM) for technical assistance with SEM-EDS; and undergraduate assistants Noah Ahles, Andrea Brendalen, Silene DeCiucies, Ethan Morehouse, Emily Piche, and Geoff Schwaner for field and laboratory assistance. Many thanks to two anonymous reviewers for comments on an earlier version of this manuscript. This work was supported by NSF EAR Hydrologic Sciences 101450, and was completed as part of the dissertation requirement for R.R. Bourgault. Hubbard Brook Experimental Forest is operated and maintained by the U.S. Forest Service, Northern Research Station, Newtown Square, PA. NR 34 TC 5 Z9 6 U1 3 U2 20 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 428 EP 439 DI 10.2136/sssaj2014.05.0190 PG 12 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900008 ER PT J AU Gillin, CP Bailey, SW McGuire, KJ Gannon, JP AF Gillin, Cody P. Bailey, Scott W. McGuire, Kevin J. Gannon, John P. TI Mapping of Hydropedologic Spatial Patterns in a Steep Headwater Catchment SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NORTHERN HARDWOOD FOREST; LOGISTIC-REGRESSION APPROACH; LANDSCAPE POSITION; STREAM WATER; TERRAIN; ECOSYSTEM; MODELS; PODZOLIZATION; INDICATORS; CHEMISTRY AB A hydropedologic approach can be used to describe soil units affected by distinct hydrologic regimes. We used field observations of soil morphology and geospatial information technology to map the distribution of five hydropedologic soil units across a 42-ha forested headwater catchment. Soils were described and characterized at 172 locations within Watershed 3, the hydrologic reference catchment for the Hubbard Brook Experimental Forest, New Hampshire. Soil profiles were grouped by presence and thickness of genetic horizons. Topographic and bedrock metrics were used in a logistic regression model to estimate the probability of soil group occurrence. Each soil group occurred under specific settings that influence subsurface hydrologic conditions. The most important metrics for predicting soil groups were Euclidean distance from bedrock outcrop, topographic wetness index, bedrock-weighted upslope accumulated area, and topographic position index. Catchment-scale maps of hydropedologic units highlight regions dominated by lateral eluviation or lateral illuviation and show that only about half the catchment is dominated by podzolization processes occurring under vertical percolation at the pedon scale. A water table map shows the importance of near-stream zones, typically viewed as variable source areas, as well as more distal bedrock-controlled zones to runoff generation. Although the catchment is steep and underlain by soils developed in coarse-textured parent material, patterns of groundwater incursion into the solum indicate that well-drained soils are restricted to deeper soils away from shallow bedrock and the intermittent stream network. Hydropedologic units can be a valuable tool for informing watershed management, soil C accounting, and understanding biogeochemical processes and runoff generation. C1 [Gillin, Cody P.; McGuire, Kevin J.] Virginia Tech, Dep Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Bailey, Scott W.] US Forest Serv, No Res Stn, North Woodstock, NH 03262 USA. [McGuire, Kevin J.] Virginia Tech, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. [Gannon, John P.] Western Carolina Univ, Geosci & Nat Resources Dep, Cullowhee, NC 28723 USA. RP Bailey, SW (reprint author), US Forest Serv, No Res Stn, 234 Mirror Lake Rd, North Woodstock, NH 03262 USA. EM swbailey@fs.fed.us RI McGuire, Kevin/E-7770-2010; OI McGuire, Kevin/0000-0001-5751-3956; Bailey, Scott/0000-0002-9160-156X; Gannon, John/0000-0002-4595-3214 FU National Science Foundation (NSF) Long-Term Ecological Research [DEB 1114804]; Hydrologic Sciences [EAR 1014507]; Research Experience for Undergraduate programs [DBI/EAR 0754678] FX Financial support was provided by the National Science Foundation (NSF) Long-Term Ecological Research (DEB 1114804), Hydrologic Sciences (EAR 1014507), and Research Experience for Undergraduate (DBI/EAR 0754678) programs. The LiDAR data were collected by Photo Science, Inc., for the White Mountain National Forest. Field work was partially conducted by Patricia Brousseau, Kaitland Harvey, Margaret Zimmer, Rebecca Bourgault, Margaret Burns, and Geoffrey Schwaner. The Hubbard Brook Experimental Forest is operated and maintained by the US Forest Service, Northern Research Station, Newtown Square, PA, and is part of the NSF Long-Term Ecological Research network. NR 62 TC 5 Z9 5 U1 6 U2 31 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 440 EP 453 DI 10.2136/sssaj2014.05.0189 PG 14 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900009 ER PT J AU Cade-Menun, BJ He, ZQ Zhang, HL Endale, DM Schomberg, HH Liu, CW AF Cade-Menun, Barbara J. He, Zhongqi Zhang, Hailin Endale, Dinku M. Schomberg, Harry H. Liu, Corey W. TI Stratification of Phosphorus Forms from Long-Term Conservation Tillage and Poultry Litter Application SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NUCLEAR-MAGNETIC-RESONANCE; P-31 NMR-SPECTROSCOPY; SOIL-PHOSPHORUS; INOSITOL HEXAKISPHOSPHATE; PHOSPHATASE-ACTIVITIES; ANIMAL MANURES; SPECIATION; IDENTIFICATION; FERTILIZATION; SUBSURFACE AB Phosphorus stratification leaves high P concentrations at the soil surface, which are vulnerable to loss in runoff. Understanding P forms at the soil surface may help control P loss, but little information is available on how P forms stratify in soils. We used P-31 nuclear magnetic resonance (NMR) spectroscopy and chemical analysis to characterize P forms and the elements controlling P cycling in Cecil soil (a fine, kaolinitic, thermic Typic Kanhapludult) that became P stratified after 11 yr of cropping management conducted under combinations of tillage (conventional and no-till, CT and NT, respectively) and inorganic chemical (CF) and poultry litter (PL) fertilization. Samples from three soil depths (0-2.5, 2.5-5, and 5-15 cm) were sequentially extracted in NaOH-ethylenediaminetetraacetic acid (EDTA) and HCl before P-31-NMR spectroscopy. The NaOH-EDTA extraction recovered 63 to 83% of the total P in a range of P forms, and HCl recovered 2 to 16% of the total P, mainly orthophosphate. Phosphorus forms and concentrations were evenly distributed in soil layers only in the CT treatment with CF. Stratification of orthophosphate and phytate occurred in NT samples fertilized with PL and CF, with the highest concentrations in the top 2.5 cm. Increased orthophosphate and phytate concentrations were detected at the soil surface for CT-PL samples, indicating that PL is not as well mixed by tillage as CF. These results suggest that P fertilizers should be placed below the soil surface under NT to avoid stratification and thus reduce potential P loss in surface runoff. C1 [Cade-Menun, Barbara J.] Agr & Agri Food Canada, Swift Current, SK S9H 3X2, Canada. [He, Zhongqi] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Zhang, Hailin] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Endale, Dinku M.] USDA ARS, Southeast Watershed Res Lab, Tifton, GA 31793 USA. [Schomberg, Harry H.] USDA ARS, Sustainable Agr Syst Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA. [Liu, Corey W.] Stanford Univ, Stanford Magnet Resonance Lab, Stanford, CA 94305 USA. RP Cade-Menun, BJ (reprint author), Agr & Agri Food Canada, Swift Current, SK S9H 3X2, Canada. EM Barbara.Cade-Menun@agr.gc.ca NR 62 TC 10 Z9 10 U1 6 U2 28 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 504 EP 516 DI 10.2136/sssaj2014.08.0310 PG 13 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900017 ER PT J AU Guzman, J Al-Kaisi, M Parkin, T AF Guzman, Jose Al-Kaisi, Mahdi Parkin, Timothy TI Greenhouse Gas Emissions Dynamics as Influenced by Corn Residue Removal in Continuous Corn System SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NITROUS-OXIDE EMISSIONS; CARBON-DIOXIDE EMISSION; LONG-TERM TILLAGE; SOIL CARBON; SOYBEAN ROTATION; AGRICULTURAL SOILS; CROPPING SYSTEMS; MANAGEMENT; FERTILIZATION; TEMPERATURE AB The removal of corn residue for bioethanol may require changes in current tillage and fertilization practices to minimize potential alterations to the soil environment that may lead to increase in greenhouse gas (GHG) emission. The objectives of this study were to examine how tillage, N fertilization rates, residue removal, and their interactions affect CO2, and N2O soil surface emissions. Greater CO2 emission coincided with higher soil temperatures typically observed with conventional tillage (CT) compared with no-tillage (NT), resulting in greater annual cumulative CO2 emission in CT (18.1 CO2 Mg ha(-1) yr(-1)) compared with NT (16.2 CO2 Mg ha(-1) yr(-1)) in 2009 and 2010 across sites. However, drier soil conditions during the growing season in 2011 lead to higher soil temperatures compared with 2009 and 2010. Consequently, annual cumulative CO2 emission from NT with 50 and 100% residue removal was (19.5 CO2 Mg ha(-1) yr(-1)) greater than that from CT (17.8 CO2 Mg ha(-1) yr(-1)) across all residue removal rates and from NT (17.5 CO2 Mg ha(-1) yr(-1)) with no residue removal, respectively across all N rates in the Ames central site (AC) in 2011. In the Armstrong southwest site (ASW) site, there were no significant differences between tillage or residue removal rates for annual cumulative CO2 emission (19.9 CO2 Mg ha(-1) yr(-1)) in 2011. Although N2O emission was considerably lower than CO2 emission, differences in N fertilization rates did have a significant impact on global warming potential once these gases were converted on the basis of their radiative forcing of the atmosphere. C1 [Guzman, Jose] Ohio State Univ, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. [Al-Kaisi, Mahdi] Iowa State Univ, Agron, Ames, IA 50011 USA. [Parkin, Timothy] ARS, USDA, Ames, IA 50011 USA. [Parkin, Timothy] Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Guzman, J (reprint author), Ohio State Univ, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. EM guzman.96@osu.edu FU Iowa State University Agronomy Department Endowment FX Support for this research project was provided by the Iowa State University Agronomy Department Endowment. NR 58 TC 3 Z9 3 U1 1 U2 26 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 612 EP 625 DI 10.2136/sssaj2014.07.0298 PG 14 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900029 ER PT J AU Mikha, MM Hergert, GW Benjamin, JG Jabro, JD Nielsen, RA AF Mikha, Maysoon M. Hergert, Gary W. Benjamin, Joseph G. Jabro, Jalal D. Nielsen, Rex A. TI Long-Term Manure Impacts on Soil Aggregates and Aggregate-Associated Carbon and Nitrogen SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER DYNAMICS; WHEAT CROPPING SYSTEM; CENTRAL GREAT-PLAINS; SANDY-LOAM SOIL; NO-TILLAGE; CONSERVATION TILLAGE; FERTILIZER APPLICATION; INORGANIC FERTILIZERS; CHEMICAL-PROPERTIES; MINERAL FERTILIZER AB Long-term studies document that soil properties influenced by management practices occur slowly. The objectives of this study were to evaluate 70 yr of manure (M) and commercial fertilizer (F) additions and moldboard plowing on soil organic C (SOC), soil total N (STN), water-stable aggregates (WSA), and aggregate-associated C and N. The Knorr-Holden plots have been in furrow irrigated continuous corn (Zea mays L.) since 1912 on a Tripp sandy loam (coarse-silty, mixed, superactive, mesic Aridic Haplustoll). Soil samples were collected from the 0- to 5-, 5- to 10-, 10- to 15-, and 15- to 30-cm depths in 2011. Soils were fractionated by wet sieving into four aggregate-size classes (>1000, 500-1000, 250-500, and 53-250 mm). Continuous M amendment increased the SOC in the 0- to 30-cm depth approximately 1.7-fold compared with the F treatment. The combination of F + M further increased SOC in the 0- to 15-cm depth by approximately 36% for the M treatment receiving 90 kg N ha(-1) of F (90 + M) and by 16% for the M treatment receiving 180 kg N ha-1 of F (180 + M) compared with the 15- to 30-cm depth. Macroaggregates increased with M and F + M when compared with F with the corresponding increase in microaggregate quantities associated with the F and no-N treatment. In the 0- to 30-cm depth, microaggregates were approximately 1.8 to 4.9 times greater than the macroaggregates. Aggregate-associated C masses were greater in microaggregates than in macroaggregates, which reflects greater amounts of microaggregates present in the soil. A significant, positive correlation was observed between SOC and aggregate-associated C. Overall, the addition of manure-based amendments, with or without F, increased SOC and enhanced aggregate stability. C1 [Mikha, Maysoon M.; Benjamin, Joseph G.] ARS, USDA, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Hergert, Gary W.; Nielsen, Rex A.] Univ Nebraska, Panhandle Res & Ext Ctr, Scottsbluff, NE 69361 USA. [Jabro, Jalal D.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Mikha, MM (reprint author), ARS, USDA, Cent Great Plains Res Stn, 40335 Rd GG, Akron, CO 80720 USA. EM Maysoon.Mikha@ars.usda.gov NR 47 TC 6 Z9 7 U1 6 U2 50 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 626 EP 636 DI 10.2136/sssaj2014.09.0348 PG 11 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900030 ER PT J AU Veum, KS Sudduth, KA Kremer, RJ Kitchen, NR AF Veum, Kristen S. Sudduth, Kenneth A. Kremer, Robert J. Kitchen, Newell R. TI Estimating a Soil Quality Index with VNIR Reflectance Spectroscopy SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID MANAGEMENT ASSESSMENT FRAMEWORK; FILLED PORE-SPACE; ORGANIC-MATTER; AGRICULTURAL MANAGEMENT; CHLOROFORM FUMIGATION; NIR SPECTROSCOPY; TILLAGE SYSTEMS; IN-SITU; CARBON; SPECTRA AB Sensor-based approaches to assessment and quantification of soil quality are important to facilitate cost-effective, site-specific soil management. The objective of this research was to evaluate the ability of visible, near-infrared (VNIR) diffuse reflectance spectroscopy to estimate multiple soil quality indicators (SQIs) and Soil Management Assessment Framework (SMAF) scores. A total of 234 soil samples from two depths (0-5 and 5-15 cm) were obtained in 2008 from 17 agricultural management systems located in the Central Claypan Region of Missouri, USA. The VNIR spectra were obtained on oven-dried and field-moist soil, and calibration models were developed with partial least squares (PLS) regression. Models were evaluated using the coefficient of determination (R-2), residual prediction deviation (RPD), and the ratio of prediction error to interquartile range (RPIQ). The most reliable estimation results were achieved using oven-dry soil for organic C, beta-glucosidase, total N, the biological SMAF score, the organic C score, and the beta-glucosidase score (R-2 >= 0.76, RPD >= 2.0, RPIQ >= 3.2). Using field-moist soils, the most reliable estimation results were achieved for organic C and the organic C score (R-2 >= 0.80, RPD >= 2.1, RPIQ >= 3.6). Incorporating the bulk density score and P score as auxiliary variables with the VNIR spectra improved estimation of the overall SMAF soil quality score for oven-dry soil (R-2 = 0.76, RPD = 2.0, RPIQ = 3.1) and field-moist soil (R-2 = 0.75, RPD = 1.9, RPIQ = 2.8). These results demonstrate the robustness of VNIR estimation of biological SQIs, and illustrate the potential for rapid, in-field quantification of soil quality by fusing VNIR sensors with auxiliary data obtained from complementary sensors or supplemental analyses. C1 [Veum, Kristen S.; Sudduth, Kenneth A.; Kitchen, Newell R.] ARS, USDA, Cropping Syst & Water Qual Unit, Columbia, MO 65211 USA. [Kremer, Robert J.] Univ Missouri, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. RP Veum, KS (reprint author), ARS, USDA, Cropping Syst & Water Qual Unit, 269 Agr Engn Bldg, Columbia, MO 65211 USA. EM Kristen.veum@ars.usda.go NR 64 TC 2 Z9 2 U1 8 U2 27 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 637 EP 649 DI 10.2136/sssaj2014.09.0390 PG 13 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900031 ER PT J AU Gesch, KR Wells, RR Cruse, RM Momm, HG Dabney, SM AF Gesch, Karl R. Wells, Robert R. Cruse, Richard M. Momm, Henrique G. Dabney, Seth M. TI Quantifying Uncertainty of Measuring Gully Morphological Evolution with Close-Range Digital Photogrammetry SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID STRUCTURE-FROM-MOTION; SOIL-EROSION; GRAVEL-BED; RECONSTRUCTION; PHOTOGRAPHY; DEPOSITION; ACCURACY; SURFACE; SYSTEM; MODEL AB Measurement of geomorphic change may be of interest to researchers and practitioners in a variety of fields, including geology, geomorphology, hydrology, engineering, and soil science. Landscapes are often represented by digital elevation models. Surface models generated of the same landscape over a time interval can be compared to estimate geomorphic evolution. Any such morphological estimate of change in a landform should include a range of probable values based on the quality of the digital elevation models that represent the surface of interest. This study sought to determine the uncertainty associated with detecting changes in reaches of ephemeral gullies with close-range digital photogrammetry. An experimental surface was constructed, surveyed, and photographed. The images were used as input to photogrammetry software to generate point clouds, which were then analyzed to determine the quality of elevation data generated by the photogrammetric technique. For individual point clouds the 2 sigma relative vertical accuracy was determined to equal 0.916 mm, and the 2 sigma absolute (geo-referenced) vertical accuracy was computed as 8.26 cm, and the 95% confidence range (2 sigma uncertainty) of detecting elevation change between two point clouds was determined to be +/- 1.29 to +/- 2.55 mm, depending on relief. These values could be applied to volumetrically derived estimates of geomorphic change as an uncertainty range. The high vertical accuracy and small uncertainty in elevation change determined in this study suggest that close-range digital photogrammetry is an effective and acceptable method to accurately detect small changes in ephemeral gullies or other geomorphic features of interest. C1 [Gesch, Karl R.; Cruse, Richard M.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Wells, Robert R.; Dabney, Seth M.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Momm, Henrique G.] Middle Tennessee State Univ, Dept Geosci, Murfreesboro, TN 37132 USA. RP Gesch, KR (reprint author), Iowa State Univ, Dept Agron, 2104 Agron Hall, Ames, IA 50011 USA. EM kgesch@iastate.edu FU NIWQP Grant from USDA-National Institute of Food and Agriculture [2012-03654] FX The authors thank Kevin Cole for sharing expertise and equipment to facilitate the RTK-GPS survey. This research was funded in part by NIWQP Grant No. 2012-03654 from the USDA-National Institute of Food and Agriculture. NR 43 TC 2 Z9 3 U1 4 U2 30 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 650 EP 659 DI 10.2136/sssaj2014.10.0396 PG 10 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900032 ER PT J AU Locke, MA Krutz, LJ Steinriede, RW Testa, S AF Locke, Martin A. Krutz, L. Jason Steinriede, R. Wade, Jr. Testa, Sam, III TI Conservation Management Improves Runoff Water Quality: Implications for Environmental Sustainability in a Glyphosate-Resistant Cotton Production System SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID PHOSPHORUS LOSSES; SOIL-PHOSPHORUS; TILLAGE SYSTEMS; SIMULATED RAINFALL; NUTRIENT LOSSES; UNITED-STATES; EROSION; SEDIMENT; CROPS; SURFACE AB Studies suggest that coincidental adoption of both herbicide-resistant genetically modified crops (GMC) and conservation management may be mutually complementary, but integrated conservation systems with GMCs need to be assessed to balance production goals with environmental concerns. Genetically modified glyphosate-resistant cotton (Gossypium hirsutum L.) was managed on replicated experimental plots as either no-till (NT) or minimum tillage (MT) and with either no cover (NC) or rye (Secale cereal L.) cover crop (CC) from 2001 to 2007 near Stoneville, MS. Rainfall simulations in 2007 were used to evaluate water quality in runoff as influenced by management at two critical times: (i) 24 h after fertilizer application in the spring; and (ii) 24 h after tillage following crop harvest. With the exception of MT-NC in the spring (with the lowest surface plant residue coverage, 2%), runoff was higher in fall than spring. Suspended solids and turbidity in runoff were higher for tilled soil (MT) and areas with no cover crop, particularly in the fall. Tillage in the fall was the largest contributor to erosion loss. Similarly, total orthophosphate and total Kjeldahl N losses were greatest with tillage in the fall, while the lowest dissolved organic C losses in runoff were in fall with NT. Overall, NT and CC treatments reduced nutrient and solids losses. Major factors contributing to these results include recent tillage (in the fall) and coverage of the soil surface by plant residues (NT > MT, CC > NC). This study demonstrated the effectiveness of integrating cover crop and conservation tillage in reducing runoff and nutrient losses in a GMC system. C1 [Locke, Martin A.; Steinriede, R. Wade, Jr.; Testa, Sam, III] ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, Oxford, MS 38655 USA. [Krutz, L. Jason] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA. RP Locke, MA (reprint author), ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, POB 1157, Oxford, MS 38655 USA. EM martin.locke@ars.usda.gov NR 56 TC 1 Z9 2 U1 2 U2 20 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 660 EP 671 DI 10.2136/sssaj2014.09.0389 PG 12 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900033 ER PT J AU Maltais-Landry, G Scow, K Brennan, E Vitousek, P AF Maltais-Landry, G. Scow, K. Brennan, E. Vitousek, P. TI Long-Term Effects of Compost and Cover Crops on Soil Phosphorus in Two California Agroecosystems SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID MICROBIAL BIOMASS; P POOLS; SYSTEMS; AVAILABILITY; MANAGEMENT; DYNAMICS; MANURE; PHOSPHATE; NITROGEN AB Inefficient P use in agriculture results in soil P accumulation and losses to surrounding ecosystems, highlighting the need to use P inputs more efficiently. Composts reduce the need for mineral fertilizers by recycling P from wastes at the regional scale, whereas cover crops reduce soil P losses and have the potential to increase internal soil P recycling by mobilizing soil P "fixed" from previous P applications. We studied the effects of compost and cover crops on soil P in two California experiments, using one to measure the effects of a single cover crop mixture and composted poultry manure across different management practices, and the second experiment to evaluate how different cover crops (pure grass, pure mustard, or grass-legumes) and yard compost affected soil P dynamics under organic management. We determined changes in soil P dynamics 8 to 18 yr after long-term experiments were established by measuring chloroform-extractable P, P sorption capacity, P saturation, and Hedley fractions. Cover crops generally increased microbial and organic P, whereas amendment with yard compost increased resin, microbial, and organic P, with no impact of cover crops and yard compost on other pools or on P sorption. In contrast, addition of composted poultry manure significantly increased all soil P pools (microbial, organic, and inorganic) and P saturation. Our results suggest a limited, moderate, and strong role of cover crops, yard compost, and composted poultry manure, respectively, in affecting soil P in California agroecosystems. C1 [Maltais-Landry, G.; Vitousek, P.] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. [Scow, K.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Brennan, E.] ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. RP Maltais-Landry, G (reprint author), Stanford Univ, Dept Biol, Gilbert Hall, Stanford, CA 94305 USA. EM gmaltais@stanford.edu FU Natural Sciences and Engineering Research Council of Canada (NSERC); Fonds Quebecois de la Recherche sur la Nature et les Technologies (FQRNT) FX We thank E. Torbert and I. Herrera for access to field sites and archived samples in Russell Ranch; S. Kolarik, T. Canonico and M. Patterson for lab assistance; J. Bateman, E. Frossard, L. Hess, N. Lincoln and three reviewers for comments. This research was partially funded by a graduate fellowship to G. Maltais-Landry from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Fonds Quebecois de la Recherche sur la Nature et les Technologies (FQRNT). NR 33 TC 3 Z9 3 U1 12 U2 51 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 688 EP 697 DI 10.2136/sssaj2014.09.0369 PG 10 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900036 ER PT J AU D'Amore, DV Ping, CL Herendeen, PA AF D'Amore, David V. Ping, Chien-Lu Herendeen, Paul A. TI Hydromorphic Soil Development in the Coastal Temperate Rainforest of Alaska SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID WATER-TABLE DEPTHS; SOUTHEAST ALASKA; MENDENHALL GLACIER; MOISTURE REGIMES; PARENT MATERIALS; COLOR PATTERNS; MORAINES; CATENA; HYDROSEQUENCE; ELECTRODES AB Predictive relationships between soil drainage and soil morphological features are essential for understanding hydromorphic processes in soils. The linkage between patterns of soil saturation, reduction, and reductimorphic soil properties has not been extensively studied in mountainous forested terrain. We measured soil saturation and reduction during a 4-yr period in three catenas of the perhumid coastal temperate rainforest of Alaska and compared these measurements to soil morphological features of Spodosols and Histosols. Soil saturation and anaerobic conditions indicated by redox potential corresponded to low-chroma colors, Fe concentrations, and accumulation of organic matter. Hue changes from 7.5YR to 10YR, 2.5Y, and 5GY were observed, at depths corresponding to water table position and Fe depletion, in Spodosol B horizons of backslope landscape positions. The depth to the features was dependent on the distance from the top of the catena, with a consistent pattern of near-surface soil saturation evident below the topographic break of 10% slope. The mean annual water table position was 34 cm below the surface in upper slope positions and 14 cm below the surface in lower slope positions. The association of color changes confirms the influence of saturation and reduction on the soil morphology of Spodosols. The consistent patterns in soil saturation, reduction, and soil morphological features offers guidance for hydric soil identification, wetland delineation, and ecological processes related to near-surface soil saturation. C1 [D'Amore, David V.] US Forest Serv, USDA, Pacific NW Res Stn, Juneau, AK 99801 USA. [Ping, Chien-Lu] Univ Alaska Fairbanks, Agr & Forestry Expt Stn, Palmer Res Ctr, Palmer, AK 99645 USA. [Herendeen, Paul A.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. RP D'Amore, DV (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. EM ddamore@fs.fed.us FU NRCS, National Wet Soil Monitoring Program; USDA Hatch project (NIFA); Alaska State Soil Classification project FX We would like to acknowledge the NRCS, National Wet Soil Monitoring Program, the USDA Hatch project (NIFA), and the Alaska State Soil Classification project for funding assistance with this project. We would also like to acknowledge the statistical advice of Pat Cunningham and Frances Biles and thank the two reviewers and associate editor for the critical and helpful review of this manuscript. We would also like to acknowledge the contributions of the late Dr. Warren Lynn to the successful implementation of this project. NR 66 TC 2 Z9 2 U1 4 U2 14 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2015 VL 79 IS 2 BP 698 EP 709 DI 10.2136/sssaj2014.08.0322 PG 12 WC Soil Science SC Agriculture GA CE2JC UT WOS:000351640900037 ER PT J AU Dudley, NS Jones, TC James, RL Sniezko, RA Cannon, P Borthakur, D AF Dudley, Nicklos S. Jones, Tyler C. James, Robert L. Sniezko, Richard A. Cannon, Phil Borthakur, Dulal TI Applied disease screening and selection program for resistance to vascular wilt in Hawaiian Acacia koa SO SOUTHERN FORESTS LA English DT Article; Proceedings Paper CT 1st International Meeting on Sustaining the Future of Acacia Plantation Forestry CY MAR 18-21, 2014 CL Hue, VIETNAM DE Acacia koa; disease resistance; Fusarium wilt; seedling inoculation; tree improvement ID LEGUMINOSAE; CLASSIFICATION; ISLANDS AB Acacia koa is a valuable tree species economically, ecologically and culturally in Hawai'i. A vascular wilt disease of A. koa resulting from infection by the fungal pathogen Fusarium oxysporum f. sp. koae ( FOXY) causes high rates of mortality in field plantings and threatens native A. koa forests in Hawai'i. Landowners are reluctant to consider A. koa for reforestation and restoration in many areas due to the threat of FOXY. Producing seeds or propagules with genetic resistance to FOXY is vital to successful A. koa reforestation and restoration. Virulent FOXY isolates were used in seedling inoculation trials to evaluate resistance levels among A. koa families in greenhouse experiments. Seedling survival varied by family, ranging from 3% to 92%, with an overall average of 46%. One clonal and three seedling field trials were established in 2012 and 2013 using selected families based on the inoculation trials. The greenhouse screening method serves as a powerful tool to rapidly evaluate A. koa families prior to outplanting, but the field trial data are needed to further validate the results and to monitor the durability over time. The field trials will also serve as a source of germplasm for selection of other commercial traits. C1 [Dudley, Nicklos S.; Jones, Tyler C.] Hawaii Agr Res Ctr, Kunia, HI 96759 USA. [James, Robert L.] Plant Dis Consulting Northwest, Vancouver, WA USA. [Sniezko, Richard A.] USDA, Forest Serv, Dorena Genet Resource Ctr, Cottage Grove, OR USA. [Cannon, Phil] USDA, Forest Serv, Forest Hlth Protect, Vallejo, CA USA. [Borthakur, Dulal] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Mol Biosci & Bioengn, Honolulu, HI 96822 USA. RP Dudley, NS (reprint author), Hawaii Agr Res Ctr, Kunia, HI 96759 USA. EM ndudley@harc-hspa.com FU Department of Land and Natural Resources, Division of Forestry and Wildlife; US Department of Agriculture, Forest Service, State and Private Forestry, Region 5, US Department of Agriculture, Natural Resource Conservation Service, Pacific Island Area, Conservation Innovation Grant [69-9251-10-881]; US Department of Agriculture, TSTAR award [2009-04862] FX We thank Aileen Yeh, Dr James Leary and James Mayo for assistance in the field. We thank the State of Hawai'i, Department of Land and Natural Resources, Division of Forestry and Wildlife for their continued administrative, technical and financial support. The project would not be possible without the support of numerous public and private landowners in providing access to collect seed of A. koa. Additional technical support was provided by the US Forest Service, Region 5, State and Private Forestry and Region 6, Dorena Genetic Resource Center. Funding was provided by the US Department of Agriculture, Forest Service, State and Private Forestry, Region 5, US Department of Agriculture, Natural Resource Conservation Service, Pacific Island Area, Conservation Innovation Grant # 69-9251-10-881; US Department of Agriculture, TSTAR award no. 2009-04862 County of Maui, Department of Water Supply, Watershed Grants Program. The US Department of Agriculture is an equal opportunity employer and provider. NR 41 TC 0 Z9 0 U1 7 U2 10 PU NATL INQUIRY SERVICES CENTRE PTY LTD PI GRAHAMSTOWN PA 19 WORCESTER STREET, PO BOX 377, GRAHAMSTOWN 6140, SOUTH AFRICA SN 2070-2620 EI 2070-2639 J9 SOUTH FORESTS JI South. Forests PD MAR PY 2015 VL 77 IS 1 SI SI BP 65 EP 73 DI 10.2989/20702620.2015.1007263 PG 9 WC Forestry SC Forestry GA CD9JT UT WOS:000351415300010 ER PT J AU Baumhardt, RL Stewart, BA Sainju, UM AF Baumhardt, R. L. Stewart, B. A. Sainju, U. M. TI North American Soil Degradation: Processes, Practices, and Mitigating Strategies SO SUSTAINABILITY LA English DT Review ID CROP-LIVESTOCK SYSTEMS; LONG-TERM TILLAGE; WESTERN CORN-BELT; GREAT-PLAINS; NITROGEN-FERTILIZATION; ORGANIC-CARBON; NO-TILL; CONVENTIONAL-TILLAGE; CHEMICAL-PROPERTIES; FALLOW ROTATION AB Soil can be degraded by several natural or human-mediated processes, including wind, water, or tillage erosion, and formation of undesirable physical, chemical, or biological properties due to industrialization or use of inappropriate farming practices. Soil degradation occurs whenever these processes supersede natural soil regeneration and, generally, reflects unsustainable resource management that is global in scope and compromises world food security. In North America, soil degradation preceded the catastrophic wind erosion associated with the dust bowl during the 1930s, but that event provided the impetus to improve management of soils degraded by both wind and water erosion. Chemical degradation due to site specific industrial processing and mine spoil contamination began to be addressed during the latter half of the 20th century primarily through point-source water quality concerns, but soil chemical degradation and contamination of surface and subsurface water due to on-farm non-point pesticide and nutrient management practices generally remains unresolved. Remediation or prevention of soil degradation requires integrated management solutions that, for agricultural soils, include using cover crops or crop residue management to reduce raindrop impact, maintain higher infiltration rates, increase soil water storage, and ultimately increase crop production. By increasing plant biomass, and potentially soil organic carbon (SOC) concentrations, soil degradation can be mitigated by stabilizing soil aggregates, improving soil structure, enhancing air and water exchange, increasing nutrient cycling, and promoting greater soil biological activity. C1 [Baumhardt, R. L.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Stewart, B. A.] West Texas A&M Univ, Dryland Agr Inst, Canyon, TX 79016 USA. [Sainju, U. M.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Baumhardt, RL (reprint author), USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. EM R.Louis.Baumhardt@ars.usda.gov; bstewart@wtamu.edu; Upendra.Sainju@ars.usda.gov NR 99 TC 4 Z9 4 U1 21 U2 92 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD MAR PY 2015 VL 7 IS 3 BP 2936 EP 2960 DI 10.3390/su7032936 PG 25 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA CE5AZ UT WOS:000351843400034 ER PT J AU Spackman, E Pantin-Jackwood, M Swayne, DE Suarez, DL Kapczynski, DR AF Spackman, Erica Pantin-Jackwood, Mary Swayne, David E. Suarez, David L. Kapczynski, Darrell R. TI Impact of route of exposure and challenge dose on the pathogenesis of H7N9 low pathogenicity avian influenza virus in chickens SO VIROLOGY LA English DT Article DE Avian influenza virus; H7N9 influenza; Chicken disease; Influenza pathogenesis ID WILD BIRDS; RT-PCR; TURKEYS; ORIGIN; INFECTION; SUBTYPE; FERRETS; DUCKS; QUANTITATION; INOCULATION AB H7N9 influenza A first caused human infections in early 2013 in China. Virus genetics, histories of patient exposures to poultry, and previous experimental studies suggest the source of the virus is a domestic avian species, such as chickens. In order to better understand the ecology of this H7N9 in chickens, we evaluated the infectious dose and pathogenesis of A/Anhui/1/2013 H7N9 in two common breeds of chickens, White Leghorns (table-egg layers) and White Plymouth Rocks (meat chickens). No morbidity or mortality were observed with doses of 10(6) or 10(8) EID50/bird when administered by the upper-respiratory route, and the mean infectious dose (10(6) EID50) was higher than expected, suggesting that the virus is poorly adapted to chickens. Virus was shed at higher titers and spread to the kidneys in chickens inoculated by the intravenous route. Challenge experiments with three other human-origin H7N9 viruses showed a similar pattern of virus replication. Published by Elsevier Inc. C1 [Spackman, Erica; Pantin-Jackwood, Mary; Swayne, David E.; Suarez, David L.; Kapczynski, Darrell R.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. RP Spackman, E (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Erica.spackman@ars.usda.gov; Mary.Pantin-jackwood@ars.usda.gov; David.Swayne@ars.usda.gov; David.Suarez@ars.usda.gov; Darrell.Kapczynski@ars.usda.gov FU U.S. Department of Agriculture, ARS CRIS Project [6612-32000-063-00D]; National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA [AAI12004-001-00001] FX The authors gratefully acknowledge, Scott Lee, Diane Smith, Aniko Zsak, Mar Costa-Hurtado, Eric Shepherd, Hai Jun Jiang, Suzanne DeBlois, Mari Rodriguez, and Kira Moresco for technical assistance with this work. This research was supported by the U.S. Department of Agriculture, ARS CRIS Project 6612-32000-063-00D and with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA no. AAI12004-001-00001. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 44 TC 6 Z9 6 U1 1 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAR PY 2015 VL 477 BP 72 EP 81 DI 10.1016/j.virol.2015.01.013 PG 10 WC Virology SC Virology GA CE0HH UT WOS:000351484600008 PM 25662310 ER PT J AU Lertpaitoonpan, W Moorman, TB Ong, SK AF Lertpaitoonpan, Warisara Moorman, Thomas B. Ong, Say Kee TI Effect of Swine Manure on Sulfamethazine Degradation in Aerobic and Anaerobic Soils SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Antibiotic; Sulfonamide; Sulfamethazine; Inhibition; Metabolites; Degradation ID SULFONAMIDE ANTIMICROBIAL AGENTS; VETERINARY ANTIBIOTICS; ANIMAL MANURE; SORPTION; FATE; SULFADIAZINE; WATER; RESIDUES; KINETICS; TYLOSIN AB Degradation and fate of sulfamethazine (SMZ) were determined under aerobic and anaerobic conditions in soil with and without swine manure amendment. For both aerobic and anaerobic conditions, SMZ disappeared rapidly during the first 7 days followed by slow disappearance which may indicate that SMZ had become more persistent and less available. For soils receiving 100 mg/kg of SMZ, the percent of SMZ remaining in the soil after 63 days were between 25 and 60 %. Depending on the initial SMZ concentration, estimated half-lives for aerobic and anaerobic incubations ranged from 1.2 to 6.6 and 2.3 days to more than 63 days, respectively. Addition of manure (0.054 g/g soil) did not significantly affect the half-lives of SMZ. Inhibitory effects of SMZ on anaerobic microbial respiration were observed in unamended soil at concentrations of 50 mg/kg or higher, but only transient inhibitory effects were found in aerobic soil. Five to 22 % of the C-14[phenyl]-SMZ added were extracted at the end of the incubations while 70 to 91 % of the C-14 were converted to bound (non-extractable) forms in both manure amended and unamended soil. Only 0.1 to 1.5 % of C-14-SMZ was mineralized to (CO2)-C-14. Disappearance of SMZ in sterilized soil was not completely halted indicating possible contribution of abiotic processes to the disappearance of SMZ in soil. C1 [Lertpaitoonpan, Warisara; Ong, Say Kee] Iowa State Univ, Dept Civil Construct & Environm Engn, Ames, IA 50011 USA. [Moorman, Thomas B.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. RP Ong, SK (reprint author), Iowa State Univ, Dept Civil Construct & Environm Engn, Ames, IA 50011 USA. EM skong@iastate.edu NR 42 TC 1 Z9 1 U1 4 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD MAR PY 2015 VL 226 IS 3 AR 81 DI 10.1007/s11270-014-2286-1 PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA CD5BO UT WOS:000351102400007 ER PT J AU Katzner, T Miller, TA Rodrigue, J Shaffer, S AF Katzner, Todd Miller, Tricia A. Rodrigue, Jane Shaffer, Steven TI A Most Dangerous Game: Death and Injury to Birds from Porcupine Quills SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article DE Aquila chrysaetos; birds; Golden Eagle; porcupine; predation ID FOOD-HABITS; ERETHIZON-DORSATUM; PREY SELECTION; GOLDEN EAGLES; NATIONAL-PARK; PREDATION; PRAIRIE; NESTS AB Predation is dangerous, not only for the prey but sometimes also for the predator. Because these dangers to predators are not well understood, we document evidence of predation or scavenging by a Golden Eagle (Aquila chrysaetos) on a North American porcupine (Erethizon dorsatum) in Pennsylvania, USA, with potentially lethal consequences to the eagle. We also review published literature on incidence of porcupine quills causing injury or death to birds. At least nine species have been documented as having contact with porcupine quills. A minimum of 39% of these interactions resulted in death to the bird, demonstrating the risk birds face when interacting with porcupines. Predation of porcupines should be selected against and irregular, yet it apparently persists and is likely more common than currently recognized. C1 [Katzner, Todd; Miller, Tricia A.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Katzner, Todd; Rodrigue, Jane] US Forest Serv, No Res Stn, USDA, Parsons, WV 26287 USA. [Shaffer, Steven] Penn Dept Conservat & Nat Resources, Tuscarora State Forest, Blain, PA 17006 USA. RP Katzner, T (reprint author), US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, 970 Lusk St, Boise, ID 83706 USA. EM tkatzner@usgs.gov OI Katzner, Todd/0000-0003-4503-8435 FU Virginia Department of Game and Inland Fisheries through a State Wildlife from the U.S. Fish and Wildlife Service; West Virginia Division of Natural Resources; North Carolina Wildlife Commission; Tennessee Wildlife Resources Agency; Pennsylvania Department of Conservation and Natural Resources FX The authors' institutions provided logistical and some financial support for this project. This publication was completed in part with funds provided by the Virginia Department of Game and Inland Fisheries through a State Wildlife Grant from the U.S. Fish and Wildlife Service. Trail camera work was supported by a number of other agencies including West Virginia Division of Natural Resources, North Carolina Wildlife Commission, Tennessee Wildlife Resources Agency, Pennsylvania Department of Conservation and Natural Resources and many others. Two anonymous reviewers and Wilson Journal of Ornithology's editor Mary Bomberger Brown provided insightful reviews that strengthened the manuscript. This is Scientific Article No. 3227 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown. NR 36 TC 1 Z9 3 U1 2 U2 9 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 EI 1938-5447 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD MAR PY 2015 VL 127 IS 1 BP 102 EP 108 PG 7 WC Ornithology SC Zoology GA CD9ZM UT WOS:000351460800012 ER PT J AU Pecor, KW Lake, EC Wund, MA AF Pecor, Keith W. Lake, Ellen C. Wund, Matthew A. TI Optimal Foraging by Birds: Experiments for Secondary & Postsecondary Students SO AMERICAN BIOLOGY TEACHER LA English DT Article DE Optimal foraging; birds; feeder; optimal diet; predation risk ID PREDATION RISK; MASS; PREFERENCE; BEHAVIOR; SIZE; PREY AB Optimal foraging theory attempts to explain the foraging patterns observed in animals, including their choice of particular food items and foraging locations. We describe three experiments designed to test hypotheses about food choice and foraging habitat preference using bird feeders. These experiments can be used alone or in combination and can also provide a foundation for students to develop extensions incorporating the basic methodology. We see these experiments as most applicable in secondary and postsecondary education, but they could be adapted for a variety of educational environments and for students with a variety of backgrounds. C1 [Pecor, Keith W.; Wund, Matthew A.] Coll New Jersey, Dept Biol, Ewing, NJ 08628 USA. [Lake, Ellen C.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. RP Pecor, KW (reprint author), Coll New Jersey, Dept Biol, 2000 Pennington Rd, Ewing, NJ 08628 USA. EM pecor@tcnj.edu; ellen.lake@ars.usda.gov; wundm@tcnj.edu FU Department of Biology; School of Science at The College of New Jersey FX We thank our students for helping develop and test these experiments and providing feedback on them. Funding was provided by the Department of Biology and the School of Science at The College of New Jersey. NR 17 TC 0 Z9 0 U1 8 U2 32 PU NATL ASSOC BIOLOGY TEACHERS INC PI RESTON PA 12030 SUNRISE VALLEY DR, #110, RESTON, VA 20191 USA SN 0002-7685 EI 1938-4211 J9 AM BIOL TEACH JI Am. Biol. Teach. PD MAR PY 2015 VL 77 IS 3 BP 192 EP 197 DI 10.1525/abt.2015.77.3.7 PG 6 WC Biology; Education, Scientific Disciplines SC Life Sciences & Biomedicine - Other Topics; Education & Educational Research GA CD3PN UT WOS:000350991200007 ER PT J AU Mackay, DS Gebauer, SK Eck, PK Baer, DJ Jones, PJH AF Mackay, Dylan S. Gebauer, Sarah K. Eck, Peter K. Baer, David J. Jones, Peter J. H. TI Lathosterol-to-cholesterol ratio in serum predicts cholesterol-lowering response to plant sterol consumption in a dual-center, randomized, single-blind placebo-controlled trial SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE cholesterol lowering; interindividual variability; non-cholesterol sterols; plant sterols; randomized controlled trial ID PLASMA-LIPID LEVELS; METABOLIC SYNDROME; SURROGATE MARKERS; LDL-CHOLESTEROL; LIPOPROTEIN CHOLESTEROL; PHYTOSTEROL ESTERS; ABSORPTION; FAT; KINETICS; ASSOCIATION AB Background: Benefits of plant sterols (PS) for cholesterol lowering are compromised by large variability in efficacy across individuals. High fractional cholesterol synthesis measured by deuterium incorporation has been associated with nonresponse to PS consumption; however, prospective studies that show this association have yet to be conducted. Objective: The goal was to test whether the lathosterol-to-cholesterol ratio (L:C ratio), a surrogate marker of endogenous cholesterol synthesis, serves as an a priori predictor of cholesterol lowering in response to PS consumption. Design: Sixty-three mildly hypercholesterolemic adults who were preselected as possessing either high endogenous cholesterol synthesis [HS; n = 24; L:C = 2.03 +/- 0.39 mu mol/mmol (mean +/- SD)] or low endogenous cholesterol synthesis (LS; n = 39; L:C = 0.99 +/- 0.28 mu mol/mmol) on the basis of baseline L:C consumed 2 g PS/d or a placebo for 28 d with the use of a dual-center, single-blind, randomized crossover design. Plasma lipid and noncholesterol sterol concentrations were measured at the end of each phase. Results: PS consumption lowered total cholesterol (TC; -0.25 +/- 0.05 mmol/L; P < 0.0001) and LDL cholesterol (-0.17 +/- 0.04 mmol/L; P < 0.0001) overall. Specifically, LS individuals responded to PS treatment with a reduction in TC (-0.40 +/- 0.07 mmol/L; P < 0.0001) and LDL cholesterol (-0.29 +/- 0.05 mmol/L; P = 0.0002), whereas HS individuals failed to show cholesterol lowering (TC: -0.09 +/- 0.09 mmol/L; P = 0.2843; LDL cholesterol: -0.05 +/- 0.07 mmol/L; P = 0.4917). The odds of LS participants responding to PS consumption with cholesterol lowering better than the mean cholesterol lowering in all participants were 4.25 (95% CI: 1.242, 14.556; P = 0.0211) for TC and 3.36 (95% CI: 1.112, 10.161; P = 0.0317) for LDL cholesterol, which was higher than for HS participants. Conclusions: The L:C ratio predicts the extent of reduction in circulating TC and LDL cholesterol in response to PS consumption. Cholesterol synthesis assessment may thus have a use in identifying responders and nonresponders to PS therapy. C1 [Mackay, Dylan S.; Eck, Peter K.; Jones, Peter J. H.] Univ Manitoba, Richardson Ctr Funct Foods & Nutraceut, Winnipeg, MB, Canada. [Jones, Peter J. H.] Univ Manitoba, Dept Food Sci, Winnipeg, MB R3T 2N2, Canada. [Mackay, Dylan S.; Eck, Peter K.; Jones, Peter J. H.] Univ Manitoba, Dept Human Nutr Sci, Winnipeg, MB, Canada. [Gebauer, Sarah K.; Baer, David J.] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. RP Jones, PJH (reprint author), Richardson Ctr Funct Foods & Nutraceut, 196 Innovat Dr,Smartpk, Winnipeg, MB R3T 6C5, Canada. EM peter.jones@umanitoba.ca RI MacKay, Dylan/B-6712-2012; OI MacKay, Dylan/0000-0002-0751-1634; Eck, Peter/0000-0003-2371-9774 FU Canadian Institutes of Health Research FX Supported by the Canadian Institutes of Health Research. Placebo and plant sterol margarines were provided by Unilever Canada Inc. NR 45 TC 6 Z9 6 U1 1 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 2015 VL 101 IS 3 BP 432 EP 439 DI 10.3945/ajcn.114.095356 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CC7HR UT WOS:000350538800005 PM 25733626 ER PT J AU Ahuja, JKC Pehrsson, PR Haytowitz, DB Wasswa-Kintu, S Nickle, M Showell, B Thomas, R Roseland, J Williams, J Khan, M Nguyen, Q Hoy, K Martin, C Rhodes, D Moshfegh, A Gillespie, C Gunn, J Merritt, R Cogswell, M AF Ahuja, Jaspreet K. C. Pehrsson, Pamela R. Haytowitz, David B. Wasswa-Kintu, Shirley Nickle, Melissa Showell, Bethany Thomas, Robin Roseland, Janet Williams, Juhi Khan, Mona Quynhanh Nguyen Hoy, Kathy Martin, Carrie Rhodes, Donna Moshfegh, Alanna Gillespie, Cathleen Gunn, Janelle Merritt, Robert Cogswell, Mary TI Sodium monitoring in commercially processed and restaurant foods SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE USDA National Nutrient Database for Standard Reference (SR); United States; monitoring; sodium; sodium reduction; commercially processed foods; restaurant foods ID NUTRIENT ANALYSIS PROGRAM; USDAS NATIONAL FOOD; UNITED-STATES AB Background: Most sodium in the US diet comes from commercially processed and restaurant foods. Sodium reduction in these foods is key to several recent public health efforts. Objective: The objective was to provide an overview of a program led by the USDA, in partnership with other government agencies, to monitor sodium contents in commercially processed and restaurant foods in the United States. We also present comparisons of nutrients generated under the program to older data. Design: We track similar to 125 commercially processed and restaurant food items ("sentinel foods") annually using information from food manufacturers and periodically by nationwide sampling and laboratory analyses. In addition, we monitor >1100 other commercially processed and restaurant food items, termed "priority-2 foods" (P2Fs) biennially by using information from food manufacturers. These foods serve as indicators for assessing changes in the sodium content of commercially processed and restaurant foods in the United States. We sampled all sentinel foods nationwide and reviewed all P2Fs in 2010-2013 to determine baseline sodium concentrations. Results: We updated sodium values for 73 sentinel foods and 551 P2Fs in the USDA's National Nutrient Database for Standard Reference (releases 23-26). Sodium values changed by at least 10% for 43 of the sentinel foods, which, for 31 foods, including commonly consumed foods such as bread, tomato catsup, and potato chips, the newer sodium values were lower. Changes in the concentrations of related nutrients (total and saturated fat, total sugar, potassium, or dietary fiber) that were recommended by the 2010 Dietary Guidelines for Americans for reduced or increased consumption accompanied sodium reduction. The results of sodium reduction efforts, based on resampling of the sentinel foods or re-review of P2Fs, will become available beginning in 2015. Conclusion: This monitoring program tracks sodium reduction efforts, improves food composition databases, and strengthens national nutrition monitoring. C1 [Ahuja, Jaspreet K. C.; Pehrsson, Pamela R.; Haytowitz, David B.; Wasswa-Kintu, Shirley; Nickle, Melissa; Showell, Bethany; Thomas, Robin; Roseland, Janet; Williams, Juhi; Khan, Mona; Quynhanh Nguyen; Hoy, Kathy; Martin, Carrie; Rhodes, Donna; Moshfegh, Alanna] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Gillespie, Cathleen; Gunn, Janelle; Merritt, Robert; Cogswell, Mary] CDC, Atlanta, GA 30333 USA. RP Ahuja, JKC (reprint author), 10300 Baltimore Ave,Bldg 005,Room 205 BARC WEST, Beltsville, MD 20705 USA. EM jaspreet.ahuja@ars.usda.gov FU CDC-USDA [60-1235-0-185] FX Supported in part by CDC-USDA agreement 60-1235-0-185. NR 37 TC 6 Z9 7 U1 1 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 2015 VL 101 IS 3 BP 622 EP 631 DI 10.3945/ajcn.114.084954 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CC7HR UT WOS:000350538800027 PM 25733648 ER PT J AU Hildebrandt, E Dunn, JR Cheng, HH AF Hildebrandt, Evin Dunn, John R. Cheng, Hans H. TI The Mut UL5-I682R Marek's Disease Virus with a Single Nucleotide Mutation Within the Helicase-Primase Subunit Gene not only Reduces Virulence but also Provides Partial Vaccinal Protection Against Marek's Disease SO AVIAN DISEASES LA English DT Article DE Marek's disease; vaccine; UL5; in vitro attenuation; recombinant ID CELL-CULTURE; HERPESVIRUS; ATTENUATION; PASSAGE; STRAIN; CHICKENS; EFFICACY AB Marek's disease virus (MDV) is an oncogenic herpesvirus that afflicts chickens with the disease known as Marek's disease (MD). This virus induces tumors, nerve lesions, immunosuppression, and death of affected birds. Vaccines are the primary control method for MD but, due to the periodic evolution of field strains, it is necessary to explore the development of new MD vaccines. MD vaccines are often attenuated MDV strains generated through serial passage in vitro. We previously used experimental evolution of MDV to provide a better understanding of the genetic basis of attenuation. During complete genome sequencing of evolved MDV populations, we identified a point mutation within the UL5 helicase-primase gene and created a UL5 recombinant virus that significantly reduced disease incidence by 89%-100%. To determine if experimental evolution also identifies mutations that provide protective qualities as potential vaccine candidates, we tested the UL5 recombinant virus as a vaccine and compared its protection to commercial herpesvirus of turkey (HVT) and bivalent (HVT + SB-1) vaccines. Both commercial vaccines resulted in higher protection against MD than did the UL5 recombinant virus, although the UL5 virus did provide protection against developing MD in 46%-70% of birds challenged. This indicates that a mutation within the UL5 helicase-primase gene not only reduces virulence but also confers protection against challenge with virulent MDV, providing support that not only can experimental evolution identify candidate mutations involved in attenuation but can also identify potential candidates for use in vaccine development. C1 [Hildebrandt, Evin] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA. [Hildebrandt, Evin; Dunn, John R.; Cheng, Hans H.] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Cheng, HH (reprint author), USDA ARS, Avian Dis & Oncol Lab, 4279 E Mt Hope Rd, E Lansing, MI 48823 USA. EM hans.cheng@ars.usda.gov FU National Research Initiative Competitive grant from USDA National Institute of Food and Agriculture [2010-65119-20505] FX We would like to thank Laurie Molitor for assistance with bird work, and Spencer Jackson for Histopaque isolation of PBLs, DNA extraction, and qPCR sample preparation, as well as Lonnie Milam for excellent technical advice. This project was supported by National Research Initiative Competitive grant number 2010-65119-20505 from the USDA National Institute of Food and Agriculture. NR 18 TC 0 Z9 0 U1 0 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2015 VL 59 IS 1 BP 94 EP 97 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA CD1IQ UT WOS:000350828700016 PM 26292541 ER PT J AU Hunt, HD Dunn, JR AF Hunt, Henry D. Dunn, John R. TI The Influence of Major Histocompatibility Complex and Vaccination with Turkey Herpesvirus on Marek's Disease Virus Evolution SO AVIAN DISEASES LA English DT Article DE Marek's disease; virus; virulence; evolution; major histocompatibility complex ID STRAIN CVI 988; CHICKEN LINES; GLYCOPROTEIN L; FIELD TRIALS; RESISTANCE; MUTATIONS; TYPE-2; GENE; TRANSFORMATION; PATHOGENESIS AB Over the last five decades, the pathogenicity of the Marek's disease virus (MDV) has evolved from the relatively mild strains (mMDV) observed in the 1960s to the more severe very-virulent-plus strains currently observed in today's outbreaks. The use of vaccines to control Marek's disease (MD), but not the infection cycle, is thought to be the major influence on the evolution of MDV. Selection for genetic resistance to MD has also been employed by the industry to control MD in the commercial setting but the role of host genetics on the evolution of MDV has been difficult to investigate in the field. To investigate the influence of vaccination and host resistance we developed a laboratory model to control and assess the effects of virus and animal genetics on MDV evolution. A bacterial artificial chromosome-derived MDV (Md5B40BAC) was used for in vivo passage (IVP) through turkey herpesvirus (HVT)-vaccinated resistant (MHC-B21) and susceptible (MHC-B13) genetic chicken lines. During IVP in the vaccinated susceptible line, the disease incidence increased from 23% MD in the first IVP to 53% MD during the fifth IVP. In the vaccinated resistant line, disease incidence increased from 0% MD during the first IVP to 29% MD during the fifth IVP. Although the IVP isolates remained relatively mild in the vaccinated resistant chicken line (29% MD) they increased from 0% to 63% MD when used to challenge the vaccinated susceptible chickens. There was no corresponding increase in disease incidence when the virus passed in the vaccinated susceptible genetic line was used to challenge the vaccinated resistant line. From this series of experiments we show that a cloned MDV (Md5B40BAC) can be selected by serial IVP to induce greater disease incidence in vaccinated chickens. This increase in disease incidence occurs in both susceptible and resistant chicken lines but is more easily observed in the susceptible line. Not surprisingly, both host genetics and vaccination play a role in selecting for increased MDV virulence. Our results suggest that the progressive increase in MDV virulence is partially masked as it circulates through vaccinated resistant genetic lines, but by applying this virus to less-resistant genetic lines, virus evolution can be clearly observed. We would predict that the introduction of more-resistant genetic lines into a commercial house contaminated with MDV circulating through susceptible lines would be less likely to produce vaccine breaks than placing susceptible lines into a house in which previously the MDV was circulating through resistant genetic lines. C1 [Hunt, Henry D.; Dunn, John R.] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Dunn, JR (reprint author), USDA ARS, Avian Dis & Oncol Lab, 4279 E Mt Hope Rd, E Lansing, MI 48823 USA. EM john.dunn@ars.usda.gov NR 41 TC 2 Z9 2 U1 1 U2 8 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2015 VL 59 IS 1 BP 122 EP 129 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CD1IQ UT WOS:000350828700020 PM 26292545 ER PT J AU Day, JM Zsak, L AF Day, J. Michael Zsak, Laszlo TI Investigating Turkey Enteric Picornavirus and Its Association with Enteric Disease in Poults SO AVIAN DISEASES LA English DT Article DE turkey; enteric; picornavirus; metagenomics ID POLYMERASE-CHAIN-REACTION; MOLECULAR CHARACTERIZATION; UNITED-STATES; RT-PCR; VIRUSES; CHICKEN; IDENTIFICATION; ASTROVIRUS; CORONAVIRUS; ROTAVIRUS AB Previous research into the viral community in the poultry gastrointestinal tract has revealed a number of novel and partially described enteric viruses. It is evident that the poultry gut viral community remains minimally characterized and incompletely understood. Investigations into the microbiome of the poultry gut have provided some insight into the geographical distribution and the rapidly evolving taxonomy of the avian enteric picornaviruses. The present investigation was undertaken to produce a comparative metagenomic analysis of the gut virome from a healthy turkey flock versus a flock placed in the field. This investigation revealed a number of enteric picornavirus sequences that were present in the commercial birds in the field that were completely absent in the healthy flock. A novel molecular diagnostic assay was used to track the shedding of field strains of turkey enteric picornavirus in commercial poults inoculated with picornavirus-positive intestinal homogenates prepared from turkeys that were experiencing moderate enteric disease. The propagation of this novel enteric picornavirus in commercial poults resulted in significant reduction in weight gain, and suggests that this common inhabitant of the turkey gut may result in performance problems or enteric disease in the field. C1 [Day, J. Michael; Zsak, Laszlo] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. RP Day, JM (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Michael.day@ars.usda.gov FU USDA/ARS CRIS [6612-32000-067-00D]; United States Poultry; Egg Association FX The authors thank H. John Barnes for supplying enteric samples from the NC State TAU flock and David Rives for sister flock samples. We thank Kabel Robbins and Butterball, LLC for providing day-old poults and field samples for the study. We thank the USDA Agricultural Research Service Genomics Laboratory for high-throughput sequencing support. Laura Ferguson and Fenglan Li are acknowledged for excellent technical support. This research was supported by USDA/ARS CRIS 6612-32000-067-00D Intervention Strategies To Control and Prevent Enteric Viral Diseases of Poultry and by a grant from the United States Poultry and Egg Association. NR 24 TC 3 Z9 3 U1 3 U2 10 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2015 VL 59 IS 1 BP 138 EP 142 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CD1IQ UT WOS:000350828700022 PM 26292547 ER PT J AU Zsak, L Cha, RM Li, FL Day, JM AF Zsak, Laszlo Cha, Ra Mi Li, Fenglan Day, J. Michael TI Host Specificity and Phylogenetic Relationships of Chicken and Turkey Parvoviruses SO AVIAN DISEASES LA English DT Article DE poultry; chicken; turkey; parvovirus; phylogenetics; host range ID POLYMERASE-CHAIN-REACTION; RUNTING-STUNTING SYNDROME; POULT ENTERITIS COMPLEX; ORIGIN REOVIRUSES; COMMERCIAL CHICKEN; BROILER-CHICKENS; VIRUS; ASTROVIRUS; INFECTION; GENOME AB Inoculation of specific-pathogen-free chickens and turkeys with five chicken parvoviruses (ChPV) and five turkey parvoviruses (TuPV) resulted in productive virus replication only in the homologous host species. A phylogenetic tree based on nucleotide sequences of the VP1 gene segment revealed a host-specific clustering of the virus strains. These results suggest that the VP1 gene plays an essential role in host specificity of ChPV and TuPV strains and could be a relevant target sequence for strain classification. C1 [Zsak, Laszlo; Cha, Ra Mi; Li, Fenglan; Day, J. Michael] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Zsak, L (reprint author), ARS, Southeast Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM laszlo.zsak@ars.usda.gov NR 35 TC 3 Z9 3 U1 2 U2 6 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2015 VL 59 IS 1 BP 157 EP 161 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CD1IQ UT WOS:000350828700026 PM 26292551 ER PT J AU Jesudhasan, PR McReynolds, JL Byrd, AJ He, HQ Genovese, KJ Droleskey, R Swaggerty, CL Kogut, MH Duke, S Nisbet, DJ Praveen, C Pillai, SD AF Jesudhasan, Palmy R. McReynolds, Jackson L. Byrd, Allen J. He, Haiqi Genovese, Kenneth J. Droleskey, Robert Swaggerty, Christina L. Kogut, Michael H. Duke, Sarah Nisbet, David J. Praveen, Chandni Pillai, Suresh D. TI Electron-Beam-Inactivated Vaccine Against Salmonella Enteritidis Colonization in Molting Hens SO AVIAN DISEASES LA English DT Article DE electron beam; ionizing irradiation; vaccine; Salmonella Enteriditis; colonization ID OUTER-MEMBRANE; CECAL BACTERIA; BROILER CHICKS; UNITED-STATES; IRRADIATION; IMMUNIZATION; SPOROZOITES; PROTECTION; IMMUNITY; MALARIA AB Electron-beam (eBeam) irradiation technology has a variety of applications in modern society. The underlying hypothesis was that eBeam-inactivated Salmonella enterica serovar Enteritidis (SE) cells can serve as a vaccine to control SE colonization and shedding in poultry birds. An eBeam dose of 2.5 kGy (kilograys) was used to inactivate a high-titer (10(8) colony-forming units [CFU]) preparation of SE cells. Microscopic studies revealed that the irradiation did not damage the bacterial cell membranes. The vaccine efficacy was evaluated by administering the eBeam-killed SE cells intramuscularly (1 x 10(6) CFU/bird) into 50-wk-old single comb white leghorn hens. On day 14 postvaccination, the hens were challenged orally with live SE cells (1 x 10(9) CFU) and SE colonization of liver, spleen, ceca, and ovaries determined on day 23. Blood samples were collected on days 0, 14, and 23 postvaccination and the sera were analyzed to quantify SE-specific IgG titers. The vaccinated chickens exhibited significantly (P < 0.0001) higher SE-specific IgG antibody responses and reduced SE ceca colonization (1.46 +/- 0.39 log(10) CFU/g) compared to nonvaccinated birds (5.32 +/- 0.32 log(10) CFU/g). They also exhibited significantly lower SE colonization of the ovaries (1/30), spleen (3/30), liver (4/30), and ceca (7/30) compared to nonvaccinated birds. These results provide empirical evidence that eBeam-based SE vaccines are immunogenic and are capable of protecting chickens against SE colonization. The advantages of eBeam-based vaccine technology are that it is nonthermal, avoids the use of formalin, and can be used to generate inactivated vaccines rapidly to address strain-specific infections in farms or flocks. C1 [Jesudhasan, Palmy R.; Praveen, Chandni; Pillai, Suresh D.] Texas A&M Univ, Natl Ctr Electron Beam Res, College Stn, TX 77843 USA. [McReynolds, Jackson L.; Byrd, Allen J.; He, Haiqi; Genovese, Kenneth J.; Droleskey, Robert; Swaggerty, Christina L.; Kogut, Michael H.; Duke, Sarah; Nisbet, David J.] Texas A&M Univ, USDA ARS, Food & Feed Safety Res Unit, College Stn, TX 77843 USA. RP Pillai, SD (reprint author), Texas A&M Univ, Natl Ctr Electron Beam Res, College Stn, TX 77843 USA. EM s-pillai@tamu.edu FU Hatch funds from the Texas A&M AgriLife Research; USDA-ARS FX The research was supported by Hatch funds from the Texas A&M AgriLife Research and funding from the USDA-ARS. The technology presented in this article is covered by U.S. patent 8,173,139. NR 24 TC 1 Z9 1 U1 0 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2015 VL 59 IS 1 BP 165 EP 170 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CD1IQ UT WOS:000350828700028 PM 26292553 ER PT J AU Chen, J Zelikova, TJ Pendall, E Morgan, JA Williams, DG AF Chen, Janet Zelikova, Tamara J. Pendall, Elise Morgan, Jack A. Williams, David G. TI Daily and seasonal changes in soil amino acid composition in a semiarid grassland exposed to elevated CO2 and warming SO BIOGEOCHEMISTRY LA English DT Article DE Organic nitrogen cycle; Soil amino acids; Grasslands; Nitrogen; Global change ID ATMOSPHERIC CARBON-DIOXIDE; ORGANIC NITROGEN; FOREST PLANTS; BOREAL FOREST; FREEZE-THAW; FINE ROOTS; ECOSYSTEM; MINERALIZATION; AVAILABILITY; TUNDRA AB Soil amino acids are often an important source of nitrogen (N) for plants, and anticipated global changes, including climate warming and rising atmospheric CO2 levels, have the potential to alter plant and microbial production and consumption of this N source in soils. We determined soil amino acid composition over a 1-year period at diurnal and seasonal time scales in a multi-factor global change experiment with elevated CO2 and warming in native semiarid grassland. Soil amino acids were collected in April, May and June of 2011 and April 2012 using a soil water perfusion and extraction method that minimized soil disturbance. This was a particular advantage when taking diurnal measurements. The extracts were analyzed by ultra performance liquid chromatography. We detected 16 different soil amino acids throughout the study, and glutamine/glutamate (glu-x), arginine, serine and asparagine/aspartate (asp-x) were consistently at highest relative concentrations, comprising 3-41, 6-20, 2-22 and 7-24 % of total amino acids, respectively. No direct effects of experimental warming or elevated CO2 on soil amino acid composition were observed. However, the relative abundance of individual soil amino acids shifted diurnally and seasonally with changes in soil temperature and soil moisture. Glu-x and arginine increased and serine decreased with higher temperature, while asp-x and serine increased and arginine decreased with higher moisture. Overall, the relative abundances of soil amino acids responded more strongly to both diurnal and seasonal changes in temperature and soil moisture than to elevated atmospheric CO2 and experimental warming. C1 [Chen, Janet; Williams, David G.] Univ Wyoming, Dept Bot Ecosyst Sci & Management, Laramie, WY 82071 USA. [Chen, Janet; Zelikova, Tamara J.; Pendall, Elise; Williams, David G.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Zelikova, Tamara J.; Pendall, Elise] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Pendall, Elise] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Morgan, Jack A.] ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. RP Chen, J (reprint author), Univ Wyoming, Dept Bot Ecosyst Sci & Management, Laramie, WY 82071 USA. EM janetchen613@gmail.com RI Williams, David/A-6407-2014; OI Williams, David/0000-0003-3627-5260; Pendall, Elise/0000-0002-1651-8969 FU US Department of Agriculture Agricultural Research Service Climate Change, Soils and Emissions Program; USDA-CSREES Soil Processes Program [2008-35107-18655]; US Department of Energy Office of Science (BER), through the Terrestrial Ecosystem Science Program [DE-SC0006973]; Western Regional Center of the National Institute for Climatic Change Research; National Science Foundation (DEB) [1021559] FX Special thanks to Dan Lecain, David Smith and Erik Hardy with the USDA-ARS Rangeland Resources Research Unit for providing air and soil moisture and temperature data and for building and maintaining the PHACE project, Chandelle MacDonald and Erik A. Hobbie for internal manuscript review, David E. Legg for SAS consultation and David J. Perry and Justin Jones for UPLC consultation. This material is based upon work supported by the US Department of Agriculture Agricultural Research Service Climate Change, Soils and Emissions Program, USDA-CSREES Soil Processes Program (#2008-35107-18655), US Department of Energy Office of Science (BER), through the Terrestrial Ecosystem Science Program (#DE-SC0006973) and the Western Regional Center of the National Institute for Climatic Change Research, and by the National Science Foundation (DEB#1021559). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. NR 63 TC 0 Z9 0 U1 11 U2 49 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAR PY 2015 VL 123 IS 1-2 BP 135 EP 146 DI 10.1007/s10533-014-0057-0 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CC9GC UT WOS:000350675300009 ER PT J AU Leathers, TD Sutivisedsak, N Nunnally, MS Price, NPJ Stanley, AM AF Leathers, Timothy D. Sutivisedsak, Nongnuch Nunnally, Melinda S. Price, Neil P. J. Stanley, April M. TI Enzymatic modification of schizophyllan SO BIOTECHNOLOGY LETTERS LA English DT Article DE beta-Glucanase; Modified schizophyllan; Schizophyllan; Viscosity ID DEGRADATION AB An enzymatic method was developed for the progressive modification of the polysaccharide schizophyllan. Fungal strains Hypocrea nigricans NRRL 62555, Penicillium crustosum NRRL 62558, and Penicillium simplicissimum NRRL 62550 were previously identified as novel sources of beta-endoglucanase with specificity towards schizophyllan. Concentrated enzyme preparations from these strains showed specific activities of 1.7-4.3 U beta-glucanase/mg protein. Using dilutions of these enzymes in time course digestions, schizophyllan was progressively modified to reduced molecular weight species. Glucose and oligosaccharides were found only in the more complete digestions, and thus modified schizophyllan can be produced quantitatively, without loss, to small molecules. Permethylation analysis confirmed that modified schizophyllan retains the fundamental linkage structure of native schizophyllan. Modified schizophyllan species showed progressively reduced viscosity profiles, and all exhibited pseudoplasticity in response to shear thinning. C1 [Leathers, Timothy D.; Sutivisedsak, Nongnuch; Nunnally, Melinda S.; Price, Neil P. J.; Stanley, April M.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Leathers, TD (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM tim.leathers@ars.usda.gov FU Agricultural and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2010-65504-20377] FX The authors thank Andrew J. Thomas for expert technical assistance. This project was supported by Agricultural and Food Research Initiative Competitive Grant no. 2010-65504-20377 from the USDA National Institute of Food and Agriculture. NR 15 TC 2 Z9 2 U1 1 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD MAR PY 2015 VL 37 IS 3 BP 673 EP 678 DI 10.1007/s10529-014-1707-y PG 6 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CC5JL UT WOS:000350394800024 PM 25335747 ER PT J AU Fielding, RA Ralston, SH Rizzoli, R AF Fielding, Roger A. Ralston, Stuart H. Rizzoli, Rene TI Emerging Impact of Skeletal Muscle in Health and Disease SO CALCIFIED TISSUE INTERNATIONAL LA English DT Editorial Material C1 [Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ralston, Stuart H.] Univ Edinburgh, Rheumat Dis Unit, Edinburgh, Midlothian, Scotland. [Rizzoli, Rene] Univ Hosp Geneva, Fac Med, Bone Dis Serv, Geneva, Switzerland. RP Fielding, RA (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM roger.fielding@tufts.edu NR 4 TC 0 Z9 0 U1 1 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0171-967X EI 1432-0827 J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD MAR PY 2015 VL 96 IS 3 SI SI BP 181 EP 182 DI 10.1007/s00223-015-9964-x PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CC9GN UT WOS:000350676500001 PM 25687787 ER PT J AU Margolis, LM Rivas, DA AF Margolis, Lee M. Rivas, Donato A. TI Implications of Exercise Training and Distribution of Protein Intake on Molecular Processes Regulating Skeletal Muscle Plasticity SO CALCIFIED TISSUE INTERNATIONAL LA English DT Review DE Skeletal muscle; Exercise adaptation; Mitochondrial biogenesis; Muscle protein turnover; Dietary protein; Cellular signaling ID ESSENTIAL AMINO-ACID; MESSENGER-RNA EXPRESSION; GAMMA COACTIVATOR-1-ALPHA EXPRESSION; INDUCED MITOCHONDRIAL BIOGENESIS; RESISTANCE EXERCISE; DIETARY-PROTEIN; ENDURANCE EXERCISE; RAG GTPASES; ANABOLIC RESPONSE; CONTRACTION MODE AB To optimize its function, skeletal muscle exhibits exceptional plasticity and possesses the fundamental capacity to adapt its metabolic and contractile properties in response to various external stimuli (e.g., external loading, nutrient availability, and humoral factors). The adaptability of skeletal muscle, along with its relatively large mass and high metabolic rate, makes this tissue an important contributor to whole body health and mobility. This adaptational process includes changes in the number, size, and structural/functional properties of the myofibers. The adaptations of skeletal muscle to exercise are highly interrelated with dietary intake, particularly dietary protein, which has been shown to further potentiate exercise training-induced adaptations. Understanding the molecular adaptation of skeletal muscle to exercise and protein consumption is vital to elicit maximum benefit from exercise training to improve human performance and health. In this review, we will provide an overview of the molecular pathways regulating skeletal muscle adaptation to exercise and protein, and discuss the role of subsequent timing of nutrient intake following exercise. C1 [Margolis, Lee M.; Rivas, Donato A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. RP Rivas, DA (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. EM donato.rivas@tufts.edu OI Rivas, Donato/0000-0002-4500-6233; , Lee/0000-0002-0652-1304 FU U.S. Department of Agriculture (USDA) [58-1950-0014]; RCDC fellowship from the Boston Claude D. Pepper Center OAIC [1P30AG031679] FX This material is based upon the work supported by the U.S. Department of Agriculture (USDA), under agreement No. 58-1950-0014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. DAR is supported by a RCDC fellowship from the Boston Claude D. Pepper Center OAIC (1P30AG031679). NR 119 TC 0 Z9 0 U1 1 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0171-967X EI 1432-0827 J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD MAR PY 2015 VL 96 IS 3 SI SI BP 211 EP 221 DI 10.1007/s00223-014-9921-0 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CC9GN UT WOS:000350676500004 PM 25348078 ER PT J AU Pojednic, RM Ceglia, L Olsson, K Gustafsson, T Lichtenstein, AH Dawson-Hughes, B Fielding, RA AF Pojednic, Rachele M. Ceglia, Lisa Olsson, Karl Gustafsson, Thomas Lichtenstein, Alice H. Dawson-Hughes, Bess Fielding, Roger A. TI Effects of 1,25-Dihydroxyvitamin D-3 and Vitamin D-3 on the Expression of the Vitamin D Receptor in Human Skeletal Muscle Cells SO CALCIFIED TISSUE INTERNATIONAL LA English DT Article DE Vitamin D; Skeletal muscle; Vitamin D receptor; Human; Primary myoblasts; Aging ID LOWER-EXTREMITY FUNCTION; PHYSICAL PERFORMANCE; OLDER WOMEN; VDR; SUPPLEMENTATION; ASSOCIATION; ACTIVATION; TISSUE AB Vitamin D receptor (VDR) expression and action in non-human skeletal muscle have recently been reported in several studies, yet data on the activity and expression of VDR in human muscle cells are scarce. We conducted a series of studies to examine the (1) effect of 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) on VDR gene expression in human primary myoblasts, (2) effect of 16-week supplementation with vitamin D-3 on intramuscular VDR gene expression in older women, and (3) association between serum 25-hydroxyvitamin D (25OHD) and intramuscular VDR protein concentration in older adults. Human primary myoblasts were treated with increasing concentrations of 1,25(OH)(2)D-3 for 18 h. A dose-dependent treatment effect was noted with 1 nmol/L of 1,25OH(2)D(3) increasing intramuscular VDR mRNA expression (mean fold change +/- A SD 1.36 +/- A 0.33; P = 0.05). Muscle biopsies were obtained at baseline and 16 weeks after vitamin D-3 supplementation (4,000 IU/day) in older adults. Intramuscular VDR mRNA was significantly different from placebo after 16 weeks of vitamin D-3 (1.2 +/- A 0.99; -3.2 +/- A 1.7, respectively; P = 0.04). Serum 25OHD and intramuscular VDR protein expression were examined by immunoblot. 25OHD was associated with intramuscular VDR protein concentration (R = 0.67; P = 0.0028). In summary, our study found VDR gene expression increases following treatment with 1,25OH(2)D(3) in human myoblasts. 25OHD is associated with VDR protein and 16 weeks of supplementation with vitamin D-3 resulted in a persistent increase in VDR gene expression of vitamin D-3 in muscle tissue biopsies. These findings suggest treatment with vitamin D compounds results in sustained increases in VDR in human skeletal muscle. C1 [Pojednic, Rachele M.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ceglia, Lisa] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA USA. [Ceglia, Lisa; Dawson-Hughes, Bess] Tufts Univ, Bone Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Olsson, Karl; Gustafsson, Thomas] Karolinska Inst, Karolinska Univ Hosp, Div Clin Physiol, Stockholm, Sweden. [Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Pojednic, RM (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM rachele.pojednic@joslin.harvard.edu OI Gustafsson, Thomas/0000-0002-1559-4206; Olsson, Karl/0000-0002-3519-4816 FU USDA Agricultural Research Service [58-1950-7-707, 58-1950-0-014]; Dairy Research Institute, Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679]; Boston Nutrition/Obesity Research Center [DK046200]; NHLBI [T32HL69772] FX USDA Agricultural Research Service, under Agreement Nos. 58-1950-7-707 (to BDH and LC) and 58-1950-0-014 (to RAF); The Dairy Research Institute (R.A.F.), Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679 to R.A.F.), the Boston Nutrition/Obesity Research Center (DK046200 to R.A.F.), and an NHLBI pre-doctoral training grant (T32HL69772 to RMP). Any opinions, findings, conclusion, or recommendation expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Agriculture. NR 21 TC 11 Z9 11 U1 0 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0171-967X EI 1432-0827 J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD MAR PY 2015 VL 96 IS 3 SI SI BP 256 EP 263 DI 10.1007/s00223-014-9932-x PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CC9GN UT WOS:000350676500008 PM 25479835 ER PT J AU Gordon, AR Karshikoff, B Kimball, BA Lundstrom, JN Soop, A Sorjonen, K Lekander, M Axelsson, J Olsson, MJ AF Gordon, Amy R. Karshikoff, Bianka Kimball, Bruce A. Lundstroem, Johan N. Soop, Anne Sorjonen, Kimmo Lekander, Mats Axelsson, John Olsson, Mats J. TI The scent of disease SO CHEMICAL SENSES LA English DT Meeting Abstract CT 24th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO) CY SEP 10-13, 2014 CL Dijon, FRANCE SP European Chemorecept Res Org C1 [Gordon, Amy R.; Karshikoff, Bianka; Lundstroem, Johan N.; Soop, Anne; Sorjonen, Kimmo; Lekander, Mats; Axelsson, John; Olsson, Mats J.] Karolinska Inst, Stockholm, Sweden. [Gordon, Amy R.; Kimball, Bruce A.; Lundstroem, Johan N.] Monell Chem Senses Ctr, Philadelphia, PA 19104 USA. [Lundstroem, Johan N.] Univ Penn, Philadelphia, PA 19104 USA. [Kimball, Bruce A.] USDA, APHIS, WS, Natl Wildlife Res Ctr, Philadelphia, PA USA. [Lekander, Mats] Stockholm Univ, S-10691 Stockholm, Sweden. EM mats.j.olsson@ki.se NR 0 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0379-864X EI 1464-3553 J9 CHEM SENSES JI Chem. Senses PD MAR PY 2015 VL 40 IS 3 BP 254 EP 254 PG 1 WC Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology SC Behavioral Sciences; Food Science & Technology; Neurosciences & Neurology; Physiology GA CD3WX UT WOS:000351015600120 ER PT J AU Zolkos, SG Jantz, P Cormier, T Iverson, LR McKenney, DW Goetz, SJ AF Zolkos, Scott G. Jantz, Patrick Cormier, Tina Iverson, Louis R. McKenney, Daniel W. Goetz, Scott J. TI Projected Tree Species Redistribution Under Climate Change: Implications for Ecosystem Vulnerability Across Protected Areas in the Eastern United States SO ECOSYSTEMS LA English DT Article DE species distribution modeling; species migration; forest management; conservation ID CHANGE IMPACTS; RANGE SHIFTS; HABITAT; BIODIVERSITY; MIGRATION; RESPONSES; FORESTS; PREDICTION; DISTRIBUTIONS; ADAPTATION AB The degree to which tree species will shift in response to climate change is uncertain yet critical to understand for assessing ecosystem vulnerability. We analyze results from recent studies that model potential tree species habitat across the eastern United States during the coming century. Our goals were to quantify and spatially analyze habitat projections and their congruence under multiple climate scenarios and to assess the implications of habitat change for forest vulnerability and adaptation to climate change in and around protected areas. We assessed habitat projections of species habitat extent and forest composition for 35 tree species under climate change from 2000 to 2100 within National Park Service management units in the Appalachian Landscape Conservation Cooperative (ALCC), spanning an approximately 1,500 km latitudinal gradient. Our results show that forest composition and species ranges could change substantially under all greenhouse gas emissions scenarios and that model correspondence was stronger for projections of habitat declines than increases. Model correspondence generally increased at finer spatial scales, but varied by tree species and focal area. In the ALCC, forest composition was projected to change the most in protected area centered ecosystems (PACEs). Northeastern PACEs were projected to be suitable for tree species currently in southeastern PACEs, suggesting that intermediate suitable habitat regions could promote tree species persistence and mitigate the impacts of climate change on eastern forests. These results suggest that climate-specific management of eastern U.S. forest ecosystems will be critical but challenging, requiring integrated assessment and management of PACEs and protected areas as well as higher-resolution monitoring and modeling to inform spatially explicit management decisions within eastern U.S. parks. C1 [Zolkos, Scott G.; Jantz, Patrick; Cormier, Tina; Goetz, Scott J.] Woods Hole Res Ctr, Falmouth, MA 02540 USA. [Iverson, Louis R.] US Forest Serv, Landscape Change Res Grp, No Res Stn, USDA, Delaware, OH 43015 USA. [McKenney, Daniel W.] Great Lakes Forestry Ctr, Canadian Forest Serv, Sault Ste Marie, ON P6A 2E5, Canada. RP Zolkos, SG (reprint author), Univ Alberta, Dept Biol Sci, CW 405,Biol Sci Bldg, Edmonton, AB T6G 2E9, Canada. EM zolkos@ualberta.ca RI Goetz, Scott/A-3393-2015 OI Goetz, Scott/0000-0002-6326-4308 FU National Aeronautics and Space Administration (NASA) Applied Sciences (Ecological Forecasting) program [NNX11AP66G] FX We thank W. Monahan and N. Fisichelli for their helpful comments; Matthew Peters, Stephen Matthews, and Anantha Prasad (U.S. Forest Service, Delaware, OH) for their work in creating and distributing the data represented in Iverson and others (2008a) used in this analysis; Kevin Lawrence, Kathy Campbell, and John Pedlar for their species modeling work in the Plant Hardiness project at Natural Resources Canada, Canadian Forest Service; and the anonymous reviewers for their insights, which greatly improved this manuscript. This work was supported by the National Aeronautics and Space Administration (NASA) Applied Sciences (Ecological Forecasting) program managed by Woody Turner (Grant NNX11AP66G). NR 58 TC 6 Z9 6 U1 5 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD MAR PY 2015 VL 18 IS 2 BP 202 EP 220 DI 10.1007/s10021-014-9822-0 PG 19 WC Ecology SC Environmental Sciences & Ecology GA CD2CC UT WOS:000350880400003 ER PT J AU Elliott, KJ Vose, JM Knoepp, JD Clinton, BD Kloeppel, BD AF Elliott, Katherine J. Vose, James M. Knoepp, Jennifer D. Clinton, Barton D. Kloeppel, Brian D. TI Functional Role of the Herbaceous Layer in Eastern Deciduous Forest Ecosystems SO ECOSYSTEMS LA English DT Article DE functional group; mesophytic cove; tree growth; litterfall; forest floor; soil CO2 efflux; N-mineralization ID ORGANIC-MATTER; SOUTHERN APPALACHIANS; TEMPERATE FOREST; SOIL CARBON; PLANT DIVERSITY; NITROGEN; TRAITS; LITTER; BIODIVERSITY; RESPONSES AB The importance of the herbaceous layer in regulating ecosystem processes in deciduous forests is generally unknown. We use a manipulative study in a rich, mesophytic cove forest in the southern Appalachians to test the following hypotheses: (i) the herbaceous functional group (HFG) in mesophytic coves accelerates carbon and nutrient cycling, (ii) high litter quality input and rapid nutrient turnover associated with HFG will have a positive effect on overstory tree growth, and (iii) the HFG regulates tree regeneration with negative effects on seedling establishment due to competition for resources. We established treatment plots in a mesic, cove-hardwoods forest and removed the herbaceous flora (HR, removed twice per year) or added herbaceous organic material (OMA, once per year) for comparison to a no removal (NR) reference for a total of 14 years. The OMA treatment stimulated soil N-mineralization and increased litterfall mass and N content. OMA N-mineralization rates were more than two times greater than both the NR and HR treatments; however, we did not detect significant differences in soil CO2 efflux among treatments. Higher overstory litterfall mass and N in the OMA treatment plots indicated that overstory trees were benefiting from the enhanced soil N-mineralization. Higher overstory leaf mass and N suggests an important linkage between HR and aboveground net primary production even though this did not translate into greater tree basal area increment. We found an increase in regeneration of all tree species with HFG removal, and the response was particularly evident for Acer rubrum seedlings. C1 [Elliott, Katherine J.; Knoepp, Jennifer D.; Clinton, Barton D.] US Forest Serv, USDA, Southern Res Stn, Ctr Forest Watershed Res,Coweeta Hydrol Lab, Otto, NC 28763 USA. [Vose, James M.] N Carolina State Univ, USDA, US Forest Serv, Southern Res Stn,Ctr Integrated Forest Sci, Raleigh, NC 27695 USA. [Kloeppel, Brian D.] Western Carolina Univ, Dept Geosci & Nat Resources, Cullowhee, NC 28723 USA. RP Elliott, KJ (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Forest Watershed Res,Coweeta Hydrol Lab, 3160 Coweeta Lab Rd, Otto, NC 28763 USA. EM kelliott@fs.fed.us FU USDA Forest Service; Southern Research Station; Coweeta Hydrologic Laboratory; Coweeta LTER Project - National Science Foundation [DEB-9632854, DEB-0218001] FX We thank Patsy Clinton and Chris Sobek for assistance in field sampling and Cindi Brown and Carol Harper for chemical analyses of plant and soil samples. We also thank Drs. Chris Oishi, Jennifer Fraterrigo, and Chelcy Miniat and two anonymous reviewers for their helpful comments on this manuscript. This research was funded by USDA Forest Service, Southern Research Station, Coweeta Hydrologic Laboratory, and the Coweeta LTER Project funded by National Science Foundation grants DEB-9632854 and DEB-0218001. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the U.S Department of Agriculture of any product or service. NR 77 TC 6 Z9 7 U1 7 U2 40 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD MAR PY 2015 VL 18 IS 2 BP 221 EP 236 DI 10.1007/s10021-014-9825-x PG 16 WC Ecology SC Environmental Sciences & Ecology GA CD2CC UT WOS:000350880400004 ER PT J AU Homann, PS Bormann, BT Morrissette, BA Darbyshire, RL AF Homann, Peter S. Bormann, Bernard T. Morrissette, Brett A. Darbyshire, Robyn L. TI Postwildfire Soil Trajectory Linked to Prefire Ecosystem Structure in Douglas-Fir Forest SO ECOSYSTEMS LA English DT Article DE fine woody debris; forest management; legacy; postwildfire change; soil carbon; soil nitrogen; wildfire ID PONDEROSA PINE FORESTS; COARSE WOODY DEBRIS; CARBON STORAGE; ORGANIC-MATTER; BOREAL FORESTS; LITTER DECOMPOSITION; DOMINATED FORESTS; PRESCRIBED FIRE; P POOLS; WILDFIRE AB Changes in soil C and N pools following wildfire are quite varied, but there is little understanding of the causes of the variation. We examined how the legacies of prefire ecosystem structure may explain the variation in soil trajectories during the first decade following wildfire. Five years prior to wildfire in a southwestern Oregon forest dominated by mature Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco], ecosystem structure was experimentally manipulated by thinning or clearcutting for comparison with unthinned forest. Repeated measurements of replicated experimental units were made before wildfire and during the first decade following wildfire. In the unthinned forest, the O-horizon soil C and N pools were decreased to 24-39% of prefire levels by wildfire, then increased to 53-70% during the first year postwildfire by deposition of fire-killed needles from overstory trees. The mineral soil (0-6 cm depth) C pool was decreased by wildfire, then increased in the following decade, while no change in the N pool was detected. In contrast, in the clearcut treatment, the O-horizon soil C and N pools were nearly totally consumed during the wildfire, lacked fire-killed overstory as a source of needle and fine and coarse wood inputs, but regained 20% of prefire masses in the following decade via foliar and root inputs from regenerated shrubs and herbaceous vegetation. Surface mineral soil C and N pools were decreased 35-50% by wildfire and showed no sign of recovery during the following decade. In contrast to wildfire, unburned ecosystem structures showed no changes in O horizon and increased mineral-soil N pool in the clearcut. We propose a conceptual model of soil C and N response following wildfire that includes legacy influences resulting from prefire ecosystem structures: residual live trees that generate continual litterfall and root turnover; fire-killed trees that produce needle-fall, dead roots, and fine- and coarse-wood detritus; and surviving roots and burls that contribute to postwildfire shrub regeneration. Consideration of prefire ecosystem structure and legacies in quantitative models may improve forecasts of postwildfire C budgets at stand to regional scales. C1 [Homann, Peter S.] Western Washington Univ, Huxley Coll Environm, Bellingham, WA 98225 USA. [Bormann, Bernard T.] US Forest Serv, Ecosyst Proc Program, USDA, Pacific Northwest Forest Res Stn,Forestry Sci Lab, Corvallis, OR 97331 USA. [Morrissette, Brett A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Darbyshire, Robyn L.] Wallowa Whitman Natl Forest, Baker City, OR 97814 USA. RP Homann, PS (reprint author), Western Washington Univ, Huxley Coll Environm, 516 High St,Environm Studies Bldg,Room 522, Bellingham, WA 98225 USA. EM Peter.Homann@wwu.edu FU Oregon [DW 12936179]; Joint Fire Sciences Program [03-2-3-09, 10-1-10-18]; National Commission for Science on Sustainable Forestry [C4] FX This paper is a contribution of the USDA Forest Service, Pacific Northwest Research Station's Long-Term Ecosystem Productivity Program. Support for pre- and posttreatment, and postwildfire sampling and analysis came from the Research Station, the US Environmental Protection Agency, Environmental Research Laboratory, Corvallis, Oregon (Interagency Agreement DW 12936179), the Joint Fire Sciences Program (Grants 03-2-3-09 and 10-1-10-18), Western Washington University, the National Commission for Science on Sustainable Forestry (Grant C4), and the Rogue River-Siskiyou National Forest. We acknowledge the hard work of many individuals, including former LTEP-experiment leader Mike Amaranthus; agreement leads from Oregon State University, Kermit Cromack Jr. and Mark Harmon. This work would not have happened without the professional field and laboratory assistants from past years: Tom Bell, Aurore Chauvry, Matt Cowall, Colin Edgar, Laura Fabrey, Nate France, Nick Leahy, Kristina Muscutt, Suzanne Remillard, Vannessa Spini, Chris Stevens, and Kyle Swanson; and from recent years: Amy Barnhart, Dylan Burgess, Nick Daniel, Martyn Davies, Emma Garner, Tim Martin, and Kylie Meyer. NR 48 TC 2 Z9 2 U1 4 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD MAR PY 2015 VL 18 IS 2 BP 260 EP 273 DI 10.1007/s10021-014-9827-8 PG 14 WC Ecology SC Environmental Sciences & Ecology GA CD2CC UT WOS:000350880400007 ER PT J AU Moore, JD Ouimet, R Long, RP Bukaveckas, PA AF Moore, Jean-David Ouimet, Rock Long, Robert P. Bukaveckas, Paul. A. TI Ecological benefits and risks arising from liming sugar maple dominated forests in northeastern North America SO ENVIRONMENTAL REVIEWS LA English DT Review DE acidification; acid rain; mitigation; liming; sugar maple; restoration; ecosystem; forest; aquatic habitat ID ACIDIC ADIRONDACK LAKE; TROUT SALVELINUS-FONTINALIS; EXOTIC EARTHWORM INVASION; BROOK-EXPERIMENTAL-FOREST; ACER-SACCHARUM MARSH; FROG RANA-TEMPORARIA; LONG-TERM CHANGES; HARDWOOD FOREST; UNITED-STATES; ALLEGHENY PLATEAU AB Liming, the application of carbonate materials (e. g., CaCO3, CaMg(CO3)(2)) to soils and surface waters, has been used extensively in Europe, and to a lesser extent in Canada and the United States, to mitigate the effects of acid deposition on forest and aquatic ecosystems. This literature review was conducted to assess the effects of liming on ecologically and economically important sugar maple dominated ecosystems of northeastern North America, where it is increasingly used to treat sugar maple dieback. Potential direct and indirect effects were considered to determine whether the use of liming to revitalize these forests could negatively affect other ecological parameters, including those in adjacent aquatic habitats. Based on current scientific literature, it is not anticipated that liming at rates of 1-3 t ha(-1) would have major detrimental effects on these ecosystems. However, liming could have negative effects on northern hardwood forests with regard to earthworm invasions. The choice of liming as a mitigation tool should be made not only after weighing the potentially negative effects against the benefits of restoring sugar maple dominated stands in poorly buffered soils, but also after considering ecological components that could be lost or never recovered if an acidified forest ecosystem is not limed. C1 [Moore, Jean-David; Ouimet, Rock] Minist Forets Faune & Parcs, Direct Rech Forestiere, Quebec City, PQ G1P 3W8, Canada. [Long, Robert P.] US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. [Bukaveckas, Paul. A.] Virginia Commonwealth Univ, Ctr Environm Studies, Dept Biol, Richmond, VA 23284 USA. RP Moore, JD (reprint author), Minist Forets Faune & Parcs, Direct Rech Forestiere, 2700 Rue Einstein, Quebec City, PQ G1P 3W8, Canada. EM jean-david.moore@mffp.gouv.qc.ca OI Ouimet, Rock/0000-0003-1282-2493; Bukaveckas, Paul/0000-0002-2636-7818 FU Ministere des Ressources naturelles du Quebec [112310063] FX This research was supported by the Ministere des Ressources naturelles du Quebec (project no. 112310063). We wish to thank Denise Tousignant for reviewing the manuscript. NR 223 TC 5 Z9 5 U1 11 U2 39 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 1208-6053 EI 1181-8700 J9 ENVIRON REV JI Environ. Rev. PD MAR PY 2015 VL 23 IS 1 BP 66 EP 77 DI 10.1139/er-2014-0048 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CC9HH UT WOS:000350678700006 ER PT J AU Maccaferri, M Zhang, JL Bulli, P Abate, Z Chao, SM Cantu, D Bossolini, E Chen, XM Pumphrey, M Dubcovsky, J AF Maccaferri, Marco Zhang, Junli Bulli, Peter Abate, Zewdie Chao, Shiaoman Cantu, Dario Bossolini, Eligio Chen, Xianming Pumphrey, Michael Dubcovsky, Jorge TI A Genome-Wide Association Study of Resistance to Stripe Rust (Puccinia striiformis f. sp tritici) in a Worldwide Collection of Hexaploid Spring Wheat (Triticum aestivum L.) SO G3-GENES GENOMES GENETICS LA English DT Article DE disease resistance; genetic map; yellow rust; bread wheat; association mapping ID MULTIPLE FUNGAL PATHOGENS; ADULT-PLANT RESISTANCE; LINKAGE DISEQUILIBRIUM; WINTER-WHEAT; POLYPLOID WHEAT; MIXED-MODEL; QUANTITATIVE RESISTANCE; STRUCTURED POPULATIONS; MISSING HERITABILITY; GENETIC ARCHITECTURE AB New races of Puccinia striiformis f. sp. tritici (Pst), the causal pathogen of wheat stripe rust, show high virulence to previously deployed resistance genes and are responsible for large yield losses worldwide. To identify new sources of resistance we performed a genome-wide association study (GWAS) using a worldwide collection of 1000 spring wheat accessions. Adult plants were evaluated under field conditions in six environments in the western United States, and seedlings were tested with four Pst races. A single-nucleotide polymorphism (SNP) Infinium 9K-assay provided 4585 SNPs suitable for GWAS. High correlations among environments and high heritabilities were observed for stripe rust infection type and severity. Greater levels of Pst resistance were observed in a subpopulation from Southern Asia than in other groups. GWAS identified 97 loci that were significant for at least three environments, including 10 with an experiment-wise adjusted Bonferroni probability < 0.10. These 10 quantitative trait loci (QTL) explained 15% of the phenotypic variation in infection type, a percentage that increased to 45% when all QTL were considered. Three of these 10 QTL were mapped far from previously identified Pst resistance genes and QTL, and likely represent new resistance loci. The other seven QTL mapped close to known resistance genes and allelism tests will be required to test their relationships. In summary, this study provides an integrated view of stripe rust resistance resources in spring wheat and identifies new resistance loci that will be useful to diversify the current set of resistance genes deployed to control this devastating disease. C1 [Maccaferri, Marco; Zhang, Junli; Abate, Zewdie; Bossolini, Eligio; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Maccaferri, Marco] Univ Bologna, Dept Agr Sci DipSA, I-40127 Bologna, Italy. [Bulli, Peter; Pumphrey, Michael] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Chao, Shiaoman] USDA ARS, Fargo, ND 58105 USA. [Cantu, Dario] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Chen, Xianming] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. RP Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, 1 Shields Ave, Davis, CA 95616 USA. EM Jdubcovsky@ucdavis.edu RI Cantu, Dario /E-7658-2010; Dubcovsky, Jorge/A-4969-2008 OI Cantu, Dario /0000-0002-4858-1508; Dubcovsky, Jorge/0000-0002-7571-4345 FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029]; Borlaug Global Rust Initiative; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation [3031] FX This project was supported by the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture, the Borlaug Global Rust Initiative, the Howard Hughes Medical Institute, and the Gordon and Betty Moore Foundation grant 3031. NR 95 TC 20 Z9 20 U1 7 U2 82 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD MAR 1 PY 2015 VL 5 IS 3 BP 449 EP 465 DI 10.1534/g3.114.014563 PG 17 WC Genetics & Heredity SC Genetics & Heredity GA CC9AK UT WOS:000350659600013 PM 25609748 ER PT J AU Grubbs, N Haas, S Beeman, RW Lorenzen, MD AF Grubbs, Nathaniel Haas, Sue Beeman, Richard W. Lorenzen, Marce D. TI The ABCs of Eye Color in Tribolium castaneum: Orthologs of the Drosophila white, scarlet, and brown Genes SO GENETICS LA English DT Article DE ABC transporters; eye pigmentation; evolution of development ID MULTIPLE SEQUENCE ALIGNMENT; MELANOGASTER-CINNABAR GENE; ANOPHELES-GAMBIAE; TRANSPORTER GENE; KYNURENINE 3-MONOOXYGENASE; TRYPTOPHAN OXYGENASE; AEDES-AEGYPTI; BOMBYX-MORI; URIC-ACID; T-COFFEE AB In Drosophila melanogaster, each of the three paralogous ABC transporters, White, Scarlet and Brown, is required for normal pigmentation of the compound eye. We have cloned the three orthologous genes from the beetle Tribolium castaneum. Conceptual translations of Tribolium white (Tcw), scarlet (Tcst), and brown (Tcbw) are 51, 48, and 32% identical to their respective Drosophila counterparts. We have identified loss-of-eye-pigment strains that bear mutations in Tcw and Tcst: the Tcw gene in the ivory (i) strain carries a single-base transversion, which leads to an E -> D amino-acid substitution in the highly conserved Walker B motif, while the Tcst gene in the pearl (p) strain has a deletion resulting in incorporation of a premature stop codon. In light of these findings, the mutant strains i and p are herein renamed white(ivory) (w(i)) and scarlet(pearl) (st(p)), respectively. In addition, RNA inhibition of Tcw and Tcst recapitulates the mutant phenotypes, confirming the roles of these genes in normal eye pigmentation, while RNA interference of Tcbw provides further evidence that it has no role in eye pigmentation in Tribolium. We also consider the evolutionary implications of our findings. C1 [Grubbs, Nathaniel; Lorenzen, Marce D.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Haas, Sue; Beeman, Richard W.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Lorenzen, MD (reprint author), N Carolina State Univ, Dept Entomol, Box 7613,1566 Thomas Hall, Raleigh, NC 27695 USA. EM marce_lorenzen@ncsu.edu RI Grubbs, Nathaniel/I-1721-2016 OI Grubbs, Nathaniel/0000-0002-3631-7427 FU NCSU; National Science Foundation [MCB-1244772] FX The authors thank Terri O'Leary and Pei-Shan Wu for technical assistance, and our two reviewers for helpful comments on the manuscript. This research was supported by the Agricultural Research Service and start-up funds to MDL from NCSU. NG is supported by a grant from the National Science Foundation (MCB-1244772). The authors declare no competing interests. NG, SH, RWB and MDL conceived and designed the experiments; NG, SH and MDL performed the experiments; NG, SH and MDL analyzed the results; and NG, RWB and MDL wrote the article. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 64 TC 2 Z9 2 U1 21 U2 69 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD MAR PY 2015 VL 199 IS 3 BP 749 EP + DI 10.1534/genetics.114.173971 PG 26 WC Genetics & Heredity SC Genetics & Heredity GA CD0BA UT WOS:000350733900011 PM 25555987 ER PT J AU Ham, C Loomis, JB Champ, PA AF Ham, Charlotte Loomis, John B. Champ, Patricia A. TI Relative Economic Values of Open Space Provided by National Forest and Military Lands to Surrounding Communities SO GROWTH AND CHANGE LA English DT Article ID PROPERTY-VALUES; PRICES; QUALITY AB Open space lands are provided by a variety of entities from private individuals to the federal government and these entities make management decisions based on a very broad range of priorities. The net benefits of additional open space depend on the number, quality, and composition of existing open space in the vicinity. In areas where open space is abundant and there is a significant proportion that is federally owned, the net benefits are not well understood. In this study, the marginal willingness to pay for proximity to public open space is estimated using the hedonic property method and home sales transactions in 2007 for El Paso County Colorado, a location that boasts of having over 150,000 acres of open space. Results from a generalized spatial two-stage least squares regression indicate that homes in close proximity to open space provided locally by city, county, and state governments sell for a premium, but larger premiums are associated with proximity to the Pike National Forest and Cheyenne Mountain Air Force Station likely at least partially due to the natural amenities of the Rocky Mountains. In contrast, proximity to Fort Carson Army Installation is found to negatively affect house sales prices likely due to undesirable activities that occur on and around the installation. As for the other area of federal lands, proximity to the U.S. Air Force Academy and to Peterson Air Force Base was found to negatively affect house prices; however, these effects were not statistically significant. C1 [Ham, Charlotte] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Loomis, John B.] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. [Champ, Patricia A.] USDA Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. RP Ham, C (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. EM cham@ncsu.edu; john.loomis@colostate.edu; pchamp@fs.fed.us FU USDA Forest Service Rocky Mountain Research Station; Center for Environmental Management of Military Lands at Colorado State University FX Charlotte Ham is a postdoctoral scholar at the Department of Forestry and Environmental Resources at North Carolina State University in Raleigh, NC, USA. Her e-mail address is: cham@ncsu.edu. John B. Loomis is a professor at the Department of Agricultural and Resource Economics at Colorado State University in Fort Collins, CO, USA. Loomis's e-mail address is: john.loomis@colostate.edu. Patricia A. Champ is an economist with the USDA Forest Service Rocky Mountain Research Station in Fort Collins, CO, USA. Champ's e-mail address is: pchamp@fs.fed.us. Funding for the project was made possible through a partnership between the USDA Forest Service Rocky Mountain Research Station and the Center for Environmental Management of Military Lands at Colorado State University. We are grateful for the constructive comments provided by the anonymous reviewers for Growth and Change. NR 40 TC 1 Z9 1 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0017-4815 EI 1468-2257 J9 GROWTH CHANGE JI Growth Change PD MAR PY 2015 VL 46 IS 1 BP 81 EP 96 DI 10.1111/grow.12072 PG 16 WC Planning & Development SC Public Administration GA CD1CK UT WOS:000350811200005 ER PT J AU Kim, KC Chun, H Lai, CQ Parnell, LD Jang, Y Lee, J Ordovas, JM AF Kim, Kyong-Chol Chun, Hyejin Lai, ChaoQiang Parnell, Laurence D. Jang, Yangsoo Lee, Jongho Ordovas, Jose. M. TI The association between genetic variants of RUNX2, ADIPOQ and vertebral fracture in Korean postmenopausal women SO JOURNAL OF BONE AND MINERAL METABOLISM LA English DT Article DE Polymorphisms; Runt-related transcription factor 2 (RUNX2); Adiponectin (ADIPOQ); Osteoporosis; Vertebral fracture ID BONE-MINERAL DENSITY; RANDOMIZED CONTROLLED-TRIAL; BODY-COMPOSITION; ELDERLY-MEN; GAMMA GENE; VITAMIN-D; FAT MASS; CALCIUM; LEPTIN; BMD AB Contrary to the traditional belief that obesity acts as a protective factor for bone, recent epidemiologic studies have shown that body fat might be a risk factor for osteoporosis and bone fracture. Accordingly, we evaluated the association between the phenotypes of osteoporosis or vertebral fracture and variants of obesity-related genes, peroxisome proliferator-activated receptor-gamma (PPARG), runt-related transcription factor 2 (RUNX2), leptin receptor (LEPR), and adiponectin (ADIPOQ). In total, 907 postmenopausal healthy women, aged 60-79 years, were included in this study. BMD and biomarkers of bone health and adiposity were measured. We genotyped for four single nucleotide polymorphisms (SNPs) from four genes (PPARG, RUNX2, LEPR, ADIPOQ). A general linear model for continuous dependent variables and a logistic regression model for categorical dependent variables were used to analyze the statistical differences among genotype groups. Compared with the TT subjects at rs7771980 in RUNX2, C-carrier (TC + CC) subjects had a lower vertebral fracture risk after adjusting for age, smoking, alcohol, total calorie intake, total energy expenditure, total calcium intake, total fat intake, weight, body fat. Odds ratio (OR) and 95 % interval (CI) for the vertebral fracture risk was 0.55 (95 % CI 0.32-0.94). After adjusting for multiple variables, the prevalence of vertebral fracture was highest in GG subjects at rs1501299 in ADIPOQ (p = 0.0473). A high calcium intake (> 1000 mg/day) contributed to a high bone mineral density (BMD) in GT + TT subjects at rs1501299 in ADIPOQ (p for interaction = 0.0295). Even if the mechanisms between obesity-related genes and bone health are not fully established, the results of our study revealed the association of certain SNPs from obesity-related genes with BMD or vertebral fracture risk in postmenopausal Korean women. C1 [Kim, Kyong-Chol] Cha Univ, Chaum Hosp, Dept Family Med, Seoul, South Korea. [Chun, Hyejin] Ewha Womans Univ, Sch Med, Mokdong Hosp, Hlth Promot Ctr, Seoul, South Korea. [Lai, ChaoQiang; Parnell, Laurence D.; Ordovas, Jose. M.] Tufts Univ, Nutr Genom Lab, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jang, Yangsoo; Lee, Jongho] Yonsei Univ, Grad Program Sci Aging, Seoul 120749, South Korea. RP Kim, KC (reprint author), Cha Univ, Chaum Hosp, Dept Family Med, Seoul, South Korea. EM joyks71@gmail.com OI Chun, Hyejin/0000-0001-6241-5453 NR 31 TC 0 Z9 0 U1 1 U2 5 PU SPRINGER JAPAN KK PI TOKYO PA CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN SN 0914-8779 EI 1435-5604 J9 J BONE MINER METAB JI J. Bone Miner. Metab. PD MAR PY 2015 VL 33 IS 2 BP 173 EP 179 DI 10.1007/s00774-014-0570-1 PG 7 WC Endocrinology & Metabolism; Medicine, Research & Experimental SC Endocrinology & Metabolism; Research & Experimental Medicine GA CC9NH UT WOS:000350697000006 PM 24570271 ER PT J AU Anderson, MK Rosenthal, J AF Anderson, M. Kat Rosenthal, Jeffrey TI AN ETHNOBIOLOGICAL APPROACH TO RECONSTRUCTING INDIGENOUS FIRE REGIMES IN THE FOOTHILL CHAPARRAL OF THE WESTERN SIERRA NEVADA SO JOURNAL OF ETHNOBIOLOGY LA English DT Article DE Indian burning; chaparral; ethnobiology; fire; Native Americans; Sierra Nevada ID CALIFORNIA INDIAN TRIBES; MANAGEMENT; GROWTH; REPRODUCTION; PATTERNS; STORAGE; FOREST AB Ethnographic interviews and historical literature reviews provide evidence that the nine ethno-linguistic groups inhabiting the foothill region of the western slope of the Sierra Nevada relied on a variety of plants and animals from chaparral communities to meet their material needs and deliberately burned the chaparral to maximize its ability to produce useful products. The reasons for burning in chaparral are grouped into six ecologically-based categories, each relying on a known response to fire of the chaparral flora, community, or landscape. The densities of people in the region were high, and villages needed large quantities of chaparral-derived materials with specific qualities to make many cultural objects. Because the majority of chaparral-occurring species used as cultural resources by Native groups required frequent fire in order to exist in sufficient quantity, or to be useful, fires caused by lightning strikes were likely insufficient to meet needs. The authors posit that tribes employed intentional burning to expand the availability, abundance, and diversity of plant materials necessary for foods, medicines, and critical technologies and that they collected plants and animals from across a mosaic of periodically burned chaparral stands at different stages of ecological succession. Overall the evidence suggests that the burning of chaparral, as a major strategy for economic intensification and extensification, altered the natural fire regimes of the foothills by expanding the burn season, shortening the fire-return interval in specific areas, and enhancing the abundance and density of species that suited specific cultural objectives. C1 [Anderson, M. Kat] USDA Nat Resources Conservat Serv, Calif State Off, Davis, CA 95616 USA. [Rosenthal, Jeffrey] Far Western Anthropol Res Grp Inc, Davis, CA USA. RP Anderson, MK (reprint author), USDA Nat Resources Conservat Serv, Calif State Off, 430 G St, Davis, CA 95616 USA. EM mkanderson@ucdavis.edu NR 143 TC 0 Z9 0 U1 6 U2 59 PU SOC ETHNOBIOLOGY PI DENTON PA UNIV NORTH TEXAS, DEPT GEOGRAPHY, 1155 UNION CIRCLE 305279, DENTON, TX 76203-5017 USA SN 0278-0771 EI 2162-4496 J9 J ETHNOBIOL JI J. Ethnobiol. PD MAR PY 2015 VL 35 IS 1 SI SI BP 4 EP 36 DI 10.2993/0278-0771-35.1.4 PG 33 WC Anthropology; Biology SC Anthropology; Life Sciences & Biomedicine - Other Topics GA CD2FC UT WOS:000350889000002 ER PT J AU Kalchayanand, N Arthur, TM Bosilevac, JM Schmidt, JW Wang, R Shackelford, S Wheeler, TL AF Kalchayanand, Norasak Arthur, Terrance M. Bosilevac, Joseph M. Schmidt, John W. Wang, Rong Shackelford, Steven Wheeler, Tommy L. TI Efficacy of Antimicrobial Compounds on Surface Decontamination of Seven Shiga Toxin-Producing Escherichia coli and Salmonella Inoculated onto Fresh Beef SO JOURNAL OF FOOD PROTECTION LA English DT Article ID COMMERCIAL GROUND-BEEF; UNITED-STATES; ORGANIC-ACIDS; IMMUNOMAGNETIC SEPARATION; PROCESSING PLANTS; CARCASS SURFACES; O157-H7; PREVALENCE; INTERVENTIONS; TYPHIMURIUM AB Several antimicrobial compounds have been used in commercial meat processing plants for decontamination of pathogens on beef carcasses, but there are many commercially available, novel antimicrobial compounds that may be more effective and suitable for use in beef processing pathogen-reduction programs. Sixty-four prerigor beef flanks (cutaneous trunci) were used in a study to determine whether hypobromous acid, neutral acidified sodium chlorite, and two citric acid based antimicrobial compounds effectively reduce seven Shiga toxin producing Escherichia coli (STEC) serogroups and Salmonella on the surface of fresh beef. Two cocktail mixtures were inoculated onto prerigor beef flank surfaces. Cocktail mixture 1 was composed of STEC serogroups O26, O103, O111, O145, and O157; and cocktail mixture 2 was composed of STEC serogroups O45, O121, and O157 and Salmonella. The inoculated fresh beef flanks were subjected to spray treatments with four antimicrobial compounds. Following antimicrobial treatments, both control and treated fresh beef samples were either enumerated immediately or were stored for 48 h at 4 degrees C before enumeration. All four antimicrobial compounds caused 0.7- to 2.0-log reductions of STEC, Salmonella, aerobic plate counts, and Enterobacteriaceae. Results also indicated that the four antimicrobial compounds were as effective at reducing the six non-O157 STEC strains as they were at reducing E. coil O157:H7 on the surfaces of fresh beef. The recovery of all seven STEC strains and Salmonella in a low-inoculation study indicated that none of the four antimicrobial compounds eliminated all of the tested pathogens. C1 [Kalchayanand, Norasak; Arthur, Terrance M.; Bosilevac, Joseph M.; Schmidt, John W.; Wang, Rong; Shackelford, Steven; Wheeler, Tommy L.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Kalchayanand, N (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM norasak.kalchayanand@ars.usda.gov FU American Meat Institute Foundation FX This study was funded in part by the American Meat Institute Foundation. The authors thank Environ Tech Chemical Services, Dan Mar, Safe Food Corporation, and SteriFx Incorporated for providing their products and for their technical support. The authors also thank Bruce Jasch, Frank Reno, Gregory Smith, Sydney Brodrick, Dee Kucera, Ken Ostdiek, Dave Kohmetscher, and Ron Harris for their laboratory technical support and Jody Gallagher for secretarial support. NR 45 TC 1 Z9 1 U1 4 U2 11 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2015 VL 78 IS 3 BP 503 EP 510 DI 10.4315/0362-028X.JFP-14-268 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CC7EU UT WOS:000350531300004 PM 25719873 ER PT J AU Edrington, TS Edrington, TS Genovese, KJ Loneragan, GH Hanson, DL Nisbet, DJ AF Brown, T. R. Edrington, T. S. Genovese, K. J. Loneragan, G. H. Hanson, D. L. Nisbet, D. J. TI Oral Salmonella Challenge and Subsequent Uptake by the Peripheral Lymph Nodes in Calves SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; LACTATING DAIRY-CATTLE; IN-GROUND BEEF; PREVALENCE; SLAUGHTER; CARRIAGE; ENTERICA; CULL AB Previous attempts to infect peripheral lymph nodes (PLNs) with Salmonella via oral inoculation have been inconsistent. Therefore, we performed a series of experiments to determine whether multiple exposures to an oral challenge would result in Salmonella-positive PLN in cattle. In each of three experiments, calves were inoculated with Salmonella Montevideo. In the first experiment, calves were challenged with either no Salmonella (control), a single oral dose (similar to 10(10); PCON), or 10 consecutive doses in water (similar to 10(3); WAT). The positive control treatment resulted in an increase (P < 0.05) in the percentage of Salmonella-positive PLNs, compared with the WAT-treated and control animals. Experiments 2 and 3 were designed to additionally determine if the stress associated with feed and water deprivation influences the systemic spread of Salmonella from the gastrointestinal tract to PLNs. Following 14 days of oral inoculation (average 7.1 x 10(4) CFU/day) in experiment 1, Salmonella was recovered from one subiliac and one superficial cervical lymph node of calves that were deprived of feed and water (72 h). No treatment differences (P > 0.05) were observed between control and deprived calves. Based on the poor recovery of Salmonella from the PLNs in WAT-challenged calves in experiments 1 and 2, a higher challenge dose (average 1.2 x 10(7) CFU) was used in experiment 3. The increased dose resulted in the recovery of the challenge strain of Salmonella from the PLNs (70.8 and 75.0% of control and deprived calves, respectively). However, no treatment differences (P > 0.05) were observed between control and deprived calves. Results of this research demonstrated that a substantial oral challenge is required to produce Salmonella-positive PLNs. However, as the challenge periods examined herein were considerably shorter compared with the normal time spent by cattle in feedlots, increased exposure time to lower doses may produce the same effect observed in experiment C1 [Brown, T. R.; Edrington, T. S.; Genovese, K. J.; Nisbet, D. J.] ARS, USDA, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. [Loneragan, G. H.; Hanson, D. L.] Texas Tech Univ, Dept Anim & Food Sci, Int Ctr Food Ind Excellence, Lubbock, TX 79409 USA. RP Edrington, TS (reprint author), ARS, USDA, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM tom.edrington@ars.usda.gov NR 15 TC 0 Z9 0 U1 2 U2 3 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2015 VL 78 IS 3 BP 573 EP 578 DI 10.4315/0362-028X.JFP-14-416 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CC7EU UT WOS:000350531300014 ER PT J AU Xie, YR Raruang, Y Chen, ZY Brown, RL Cleveland, TE AF Xie, Yu-Rong Raruang, Yenjit Chen, Zhi-Yuan Brown, Robert L. Cleveland, Thomas E. TI ZmGns, a maize class I beta-1,3-glucanase, is induced by biotic stresses and possesses strong antimicrobial activity SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Article DE beta-1; 3-Glucanase; biological activity; expression profiles; Zea mays; ZmGns ID PATHOGENESIS-RELATED PROTEINS; ASPERGILLUS-FLAVUS; GENE; INFECTION; EXPRESSION; CHITINASE; SEQUENCE; KERNELS; BINDING; FRUIT AB Plant -1,3-glucanases are members of the pathogenesis-related protein 2 (PR-2) family, which is one of the 17 PR protein families and plays important roles in biotic and abiotic stress responses. One of the differentially expressed proteins (spot 842) identified in a recent proteomic comparison between five pairs of closely related maize (Zea mays L.) lines differing in aflatoxin resistance was further investigated in the present study. Here, the corresponding cDNA was cloned from maize and designated as ZmGns. ZmGns encodes a protein of 338 amino acids containing a potential signal peptide. The expression of ZmGns was detectible in all tissues studied with the highest level in silks. ZmGns was significantly induced by biotic stresses including three bacteria and the fungus Aspergillus flavus. ZmGns was also induced by most abiotic stresses tested and growth hormones including salicylic acid. In vivo, ZmGns showed a significant inhibitory activity against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and fungal pathogen Botrytis cinerea when it overexpressed in Arabidopsis. Its high level of expression in the silk tissue and its induced expression by phytohormone treatment, as well as by bacterial and fungal infections, suggest it plays a complex role in maize growth, development, and defense. C1 [Xie, Yu-Rong; Raruang, Yenjit; Chen, Zhi-Yuan] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Brown, Robert L.; Cleveland, Thomas E.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Chen, ZY (reprint author), Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. EM zchen@agcenter.lsu.edu FU USDA [58-6435-6-055] FX We thank Drs. Norimoto Murai and Jong Hyun Ham for critical reviewing of the manuscript and Nicole Hazard for technical assistance. This study was supported by USDA cooperative agreement 58-6435-6-055. Published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript number 2009-240-2574. NR 52 TC 1 Z9 2 U1 3 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD MAR PY 2015 VL 57 IS 3 BP 271 EP 283 DI 10.1111/jipb.12286 PG 13 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CD2IO UT WOS:000350900500005 PM 25251325 ER PT J AU Ngeleza, GK Muhammad, A AF Ngeleza, Guyslain K. Muhammad, Andrew TI PREFERENTIAL TRADE AGREEMENTS BETWEEN THE MONETARY COMMUNITY OF CENTRAL AFRICA AND THE EUROPEAN UNION: STUMBLING OR BUILDING BLOCKS? A GENERAL EQUILIBRIUM APPROACH SO JOURNAL OF INTERNATIONAL DEVELOPMENT LA English DT Review DE Central Africa; EU; regional trade; multilateral trade; computable general equilibrium model; F13; F53; C68 ID ECONOMIC PARTNERSHIP AGREEMENTS; REGIONALISM; LIBERALIZATION; POLICY; MULTILATERALISM; WELFARE; EU AB This paper uses a computable general equilibrium approach to simulate two opposing views describing regional trade agreements either as building blocks for or stumbling blocks to multilateral trade liberalisation. This study focuses on the regional trade agreement between the Economic and Monetary Community of Central Africa (CEMAC) and the European Union (EU). Results show that, although a regional trade agreement may slightly raise welfare among the members of the agreement, the cost to nonmembers can be high. The regional breakdown in our design considers 14 regions, allowing for country-specific analysis for one least-developed country (Democratic Republic of Congo) and one non-least-developed country (Cameroon). Multilateral liberalisation amplifies welfare gain for Cameroon. The Democratic Republic of Congo, with its weaker institutional capacity, is affected negatively. An EU-CEMAC regional free trade agreement without multilateralism produces gains for both Cameroon and the Democratic Republic of Congo. Copyright (c) 2011 John Wiley & Sons, Ltd. C1 [Ngeleza, Guyslain K.] Int Food Policy Res Inst, Dev Strategy & Governance Div, Washington, DC 20036 USA. [Muhammad, Andrew] USDA, Econ Res Serv, Washington, DC USA. RP Ngeleza, GK (reprint author), Int Food Policy Res Inst, Dev Strategy & Governance Div, 6717 Edgartown Way, Gainesville, VA 20155 USA. EM G.ngeleza@cgiar.org FU Global Trade Analysis Project FX The authors are grateful to the Global Trade Analysis Project for research support. Authors also thank you, Thomas Hertel, William Masters, Terrie Walmsley, and Badri Narayanan, for suggestions and comments. The views expressed in this paper are those of the authors, and may not be attributed to the Economic Research Service or the U.S. Department of Agriculture. NR 44 TC 0 Z9 0 U1 3 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0954-1748 EI 1099-1328 J9 J INT DEV JI J. Int. Dev. PD MAR PY 2015 VL 27 IS 2 BP 251 EP 272 DI 10.1002/jid.1838 PG 22 WC Planning & Development SC Public Administration GA CD0NJ UT WOS:000350770700006 ER PT J AU Schuller, S Francey, T Hartmann, K Hugonnard, M Kohn, B Nally, JE Sykes, J AF Schuller, S. Francey, T. Hartmann, K. Hugonnard, M. Kohn, B. Nally, J. E. Sykes, J. TI European consensus statement on leptospirosis in dogs and cats SO JOURNAL OF SMALL ANIMAL PRACTICE LA English DT Review ID CHAIN-REACTION ASSAY; DISSEMINATED INTRAVASCULAR COAGULATION; KIRSCHNERI SEROVAR-GRIPPOTYPHOSA; MICROSCOPIC AGGLUTINATION-TEST; ACUTE-RENAL-FAILURE; CLIENT-OWNED DOGS; CANINE LEPTOSPIROSIS; RISK-FACTORS; SUSPECTED LEPTOSPIROSIS; PULMONARY HEMORRHAGE AB Leptospirosis is a zoonotic disease with a worldwide distribution affecting most mammalian species. Clinical leptospirosis is common in dogs but appears to be rare in cats. Both dogs and cats, however, can shed leptospires in the urine. This is problematic as it can lead to exposure of humans. The control of leptospirosis, therefore, is important not only from an animal but also from a public health perspective. The aim of this consensus statement is to raise awareness of leptospirosis and to outline the current knowledge on the epidemiology, clinical features, diagnostic tools, prevention and treatment measures relevant to canine and feline leptospirosis in Europe. C1 [Schuller, S.; Francey, T.] Univ Bern, Vetsuisse Fac, Dept Clin Vet Med, CH-3012 Bern, Switzerland. [Hartmann, K.] Univ Munich, Med Kleintierklin, D-80539 Munich, Germany. [Hugonnard, M.] Univ Lyon, VetAgro Sup, Small Anim Internal Med, Res Unit RS2GP,USC 1233, F-69280 Marcy Letoile, France. [Kohn, B.] Free Univ Berlin, Fac Vet Med, Clin Small Anim, D-14163 Berlin, Germany. [Nally, J. E.] ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Sykes, J.] Univ Calif Davis, Dept Med & Epidemiol, Davis, CA 95616 USA. RP Schuller, S (reprint author), Univ Bern, Vetsuisse Fac, Dept Clin Vet Med, CH-3012 Bern, Switzerland. RI HUGONNARD, Marine/C-6512-2015; OI HUGONNARD, Marine/0000-0002-1421-2448; Francey, Thierry/0000-0002-6693-5365; Hartmann, Katrin/0000-0002-5256-863X FU International Society for Companion Animal Infectious Diseases (ISCAID); MSD Animal Health FX This consensus was supported by the International Society for Companion Animal Infectious Diseases (ISCAID). The authors would like to thank MSD Animal Health for financially supporting a face-to-face meeting of the consensus panel. Stephanie Knopfler (FU Berlin) is acknowledged for preparing Tables 4-6. The authors would like to thank the reviewers for their excellent guidance during the preparation of this manuscript. NR 170 TC 16 Z9 16 U1 17 U2 64 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-4510 EI 1748-5827 J9 J SMALL ANIM PRACT JI J. Small Anim. Pract. PD MAR PY 2015 VL 56 IS 3 BP 159 EP 179 DI 10.1111/jsap.12328 PG 21 WC Veterinary Sciences SC Veterinary Sciences GA CD2JX UT WOS:000350905000002 PM 25754092 ER PT J AU Xu, LY Bickhart, DM Cole, JB Schroeder, SG Song, JZ Van Tassell, CP Sonstegard, TS Liu, GE AF Xu, Lingyang Bickhart, Derek M. Cole, John B. Schroeder, Steven G. Song, Jiuzhou Van Tassell, Curtis P. Sonstegard, Tad S. Liu, George E. TI Genomic Signatures Reveal New Evidences for Selection of Important Traits in Domestic Cattle SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE cattle genome; population structure; positive selection; haplotype network ID RECENT POSITIVE SELECTION; MULTILOCUS GENOTYPE DATA; GROWTH-HORMONE RECEPTOR; DNA-SEQUENCING DATA; DAIRY-CATTLE; ARTIFICIAL SELECTION; BOS-TAURUS; WIDE ASSOCIATION; HOLSTEIN CATTLE; COMPLEX TRAITS AB We investigated diverse genomic selections using high-density single nucleotide polymorphism data of five distinct cattle breeds. Based on allele frequency differences, we detected hundreds of candidate regions under positive selection across Holstein, Angus, Charolais, Brahman, and N'Dama. In addition to well-known genes such as KIT, MC1R, ASIP, GHR, LCORL, NCAPG, WIF1, and ABCA12, we found evidence for a variety of novel and less-known genes under selection in cattle, such as LAP3, SAR1B, LRIG3, FGF5, and NUDCD3. Selective sweeps near LAP3 were then validated by next-generation sequencing. Genome-wide association analysis involving 26,362 Holsteins confirmed that LAP3 and SAR1B were related to milk production traits, suggesting that our candidate regions were likely functional. In addition, haplotype network analyses further revealed distinct selective pressures and evolution patterns across these five cattle breeds. Our results provided a glimpse into diverse genomic selection during cattle domestication, breed formation, and recent genetic improvement. These findings will facilitate genome-assisted breeding to improve animal production and health. C1 [Xu, Lingyang; Bickhart, Derek M.; Cole, John B.; Schroeder, Steven G.; Van Tassell, Curtis P.; Sonstegard, Tad S.; Liu, George E.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Xu, Lingyang; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. RP Liu, GE (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM George.Liu@ars.usda.gov RI Cole, John/J-8571-2014; OI Cole, John/0000-0003-1242-4401; Schroeder, Steven/0000-0001-9103-5150; Bickhart, Derek/0000-0003-2223-9285 FU Agriculture and Food Research Initiative (AFRI) from U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) [2011-67015-30183] FX The authors thank members of the Bovine HapMap Consortium for sharing their samples. The authors also thank Reuben Anderson and Alexandre Dimitriv for technical assistance. This work was supported in part by Agriculture and Food Research Initiative (AFRI) grant No. 2011-67015-30183 from U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 113 TC 22 Z9 23 U1 2 U2 37 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0737-4038 EI 1537-1719 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD MAR PY 2015 VL 32 IS 3 BP 711 EP 725 DI 10.1093/molbev/msu333 PG 15 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA CD2KZ UT WOS:000350908500014 PM 25431480 ER PT J AU Daimiel-Ruiz, L Klett-Mingo, M Konstantinidou, V Mico, V Aranda, JF Garcia, B Martinez-Botas, J Davalos, A Fernandez-Hernando, C Ordovas, JM AF Daimiel-Ruiz, Lidia Klett-Mingo, Mercedes Konstantinidou, Valentini Mico, Victor Aranda, Juan F. Garcia, Belen Martinez-Botas, Javier Davalos, Alberto Fernandez-Hernando, Carlos Ordovas, Jose M. TI Dietary lipids modulate the expression of miR-107, an miRNA that regulates the circadian system SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Circadian rhythm; DHA; Dietary lipids; miR-107 ID CONJUGATED LINOLEIC-ACID; POLYUNSATURATED FATTY-ACIDS; CELL-CYCLE PROGRESSION; CARDIOVASCULAR-DISEASE; MICRORNA EXPRESSION; CACO-2 CELLS; FISH-OIL; CHOLESTEROL; METABOLISM; OBESITY AB Scope: The increased prevalence of cardiovascular diseases (CVDs) has been hypothesized to be the result of an increased exposure to a host of atherogenic environmental factors, paramount among them being unhealthy dietary habits. Long-chain n-3 polyunsaturated fatty acids have been shown to have cardio protective effects, partially due to their ability to regulate gene expression. In this regard, increasing attention has been devoted to the role of miRNAs as regulators of multiple metabolic pathways whose deregulation has been associated with CVD risk. Methods and results: In this work, we investigated whether miRNA expression was regulated by docosahexanoic acid, conjugated linoleic acid, and cholesterol in Caco-2 cells. The modulated miRNAs, miR-107 was differentially expressed by all treatments and this modulation was independent of its hosting gene, PANK1, possibly through its own promoter, which contains binding sites for metabolically relevant transcription factors. Among the putative target genes of miR-107, we found some genes with key roles in circadian rhythm. Specifically, we demonstrated that binding of miR-107 to the CLOCK gene results in the deregulation of the circadian rhythm of the cells. Conclusion: Since chronodisruption has been linked to metabolic disorders such as type 2 diabetes, atherosclerosis, obesity, and CVD, our findings suggests that miR-107 could represent a new approach for pharmacological treatment of these diseases. C1 [Daimiel-Ruiz, Lidia; Klett-Mingo, Mercedes; Konstantinidou, Valentini; Mico, Victor; Garcia, Belen; Davalos, Alberto; Ordovas, Jose M.] CEI UAM CSIC, IMDEA Food Inst, Nutrit Genom Cardiovasc Dis & Obes, E-28049 Madrid, Spain. [Aranda, Juan F.; Fernandez-Hernando, Carlos] Yale Univ, Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USA. [Aranda, Juan F.; Fernandez-Hernando, Carlos] Yale Univ, Sch Med, Comparat Med Sect, Integrat Cell Signaling & Neurobiol Metab Program, New Haven, CT 06510 USA. [Martinez-Botas, Javier] Hosp Univ Ramon y Cajal, IRyCIS, Serv Bioquim Invest, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. RP Daimiel-Ruiz, L (reprint author), CEI UAM CSIC, IMDEA Food Inst, Nutrit Genom Cardiovasc Dis & Obes, E-28049 Madrid, Spain. EM Lidia.daimiel@imdea.org RI Mico, Victor/M-7763-2014; OI Mico, Victor/0000-0003-0681-7962; Davalos, Alberto/0000-0001-5709-6443; Martinez-Botas, Javier/0000-0002-5190-3619 FU Instituto de Salud Carlos III [FIS] [PI11/00315]; Sociedad Espanola de Arteriosclerosis; National Institutes of Health [R01HL107953]; US Department of Agriculture Research Service [53-K06-5-10, 58e1950-9-001]; Fundacion Salud - Premio Merck-Serono de Investigacion FX We thank Jesus Herranz for his assistance with the cosinor analysis of the circadian rhythm. We also thank Roberto Martin for helping with the graphical representation of the microarray data. This work was supported by the direct finding of IMDEA-Food Institute through Comunidad de Madrid and European Union Structural Funds. Additional funding was provided by the Instituto de Salud Carlos III [FIS, PI11/00315] and the Sociedad Espanola de Arteriosclerosis to AD; the National Institutes of Health [R01HL107953] to CF-H; and the US Department of Agriculture Research Service [53-K06-5-10, 58e1950-9-001] and Fundacion Salud 2000 - Premio Merck-Serono de Investigacion 2012 to JMO. NR 68 TC 6 Z9 6 U1 3 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD MAR PY 2015 VL 59 IS 3 BP 552 EP 565 DI 10.1002/mnfr.201400616 PG 14 WC Food Science & Technology SC Food Science & Technology GA CD0LF UT WOS:000350763500017 PM 25522185 ER PT J AU Steelman, TA McCaffrey, SM Velez, ALK Briefel, JA AF Steelman, Toddi A. McCaffrey, Sarah M. Velez, Anne-Lise Knox Briefel, Jason Alexander TI What information do people use, trust, and find useful during a disaster? Evidence from five large wildfires SO NATURAL HAZARDS LA English DT Article DE Public information; Communication; Used; Useful; Trustworthy ID RISK COMMUNICATION; SOCIAL MEDIA; CRISIS COMMUNICATION; MANAGEMENT; PERCEPTION; PREPAREDNESS; CREDIBILITY; COMMUNITIES; STRATEGIES; CONTEXT AB The communication system through which information flows during a disaster can be conceived of as a set of relationships among sources and recipients who are concerned about key information characteristics. The recipient perspective is often neglected within this system. In this article, we explore recipient perspectives related to what information was used, useful, and trustworthy in a wildfire context. Using a survey (n = 873) on five large wildfires in 2009 and 2010, we found significant gaps between the sources that were used by the most respondents and those that that they rated as useful or trustworthy. The sources that were used most before the fires were highly correlated with the sources that were used most during the fire. C1 [Steelman, Toddi A.] Univ Saskatchewan, Sch Environm & Sustainabil, Saskatoon, SK S7H 2J5, Canada. [McCaffrey, Sarah M.] US Forest Serv, No Res Stn, USDA, Evanston, IL 60626 USA. [Velez, Anne-Lise Knox] N Carolina State Univ, Sch Publ & Int Affairs, Dept Publ Adm, Raleigh, NC 27695 USA. RP Steelman, TA (reprint author), Univ Saskatchewan, Sch Environm & Sustainabil, 329 Kirk Hall,117 Sci Pl, Saskatoon, SK S7H 2J5, Canada. EM Toddi.Steelman@usask.ca; smccaffrey@fs.fed.us; aknox2@ncsu.edu; Jason.Briefel@gmail.com FU Joint Fire Science Program [8-1-2 4-01]; USFS Northern Research Station; USFS Rocky Mountain Research Station; National Fire Protection Association FX This work was funded by the Joint Fire Science Program Grant Number 8-1-2 4-01, the USFS Northern Research Station, the USFS Rocky Mountain Research Station, and the National Fire Protection Association. All views expressed in this article are those of the authors. NR 69 TC 3 Z9 3 U1 5 U2 40 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0921-030X EI 1573-0840 J9 NAT HAZARDS JI Nat. Hazards PD MAR PY 2015 VL 76 IS 1 BP 615 EP 634 DI 10.1007/s11069-014-1512-x PG 20 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Water Resources SC Geology; Meteorology & Atmospheric Sciences; Water Resources GA CC4LZ UT WOS:000350326200032 ER PT J AU Kang, J Thakali, KM Jensen, GS Wu, XL AF Kang, Jie Thakali, Keshari M. Jensen, Gitte S. Wu, Xianli TI Phenolic Acids of the Two Major Blueberry Species in the US Market and Their Antioxidant and Anti-inflammatory Activities SO PLANT FOODS FOR HUMAN NUTRITION LA English DT Article DE Anti-inflammation; Antioxidant; Blueberries; HPLC-MS; Phenolic acid ID IN-VIVO ANTIOXIDANT; NATURAL-PRODUCTS; CAPACITY; SERUM; CONSUMPTION; FRUITS; FOODS; POLYPHENOLS; BERRIES; HEALTH AB Highbush (cultivated) and lowbush (wild) are the two major blueberry species in the US market. Eight phenolic acids were detected and quantified from these two species by HPLC-MS. Chlorogenic acid was found to be the predominant phenolic acid in both species, with 0.44 mg/g fresh weight in lowbush blueberries and 0.13 mg/g fresh weight in highbush blueberries. Total phenolic content in lowbush blueberries is over three times higher than that of highbush blueberries. The phenolic acid mixtures representing those in the two species were prepared by using authentic standards to assess their contribution to total antioxidant and anti-inflammatory activities of the whole berries. Neither lowbush nor highbush blueberry phenolic acid mixture contributed significantly to the total antioxidant capacity of their relevant whole berries measured by oxygen radical absorbance capacity (ORAC). Both phenolic acid mixtures were able to enter the cell and showed in cell antioxidant activities from the cell based antioxidant protection of erythrocytes (CAP-e) assay. Lowbush blueberry phenolic acid mixture was found to show anti-inflammatory activities by inhibiting the nuclear factor-kappa B (NF-kappa B) activation and the production of inflammatory cytokines (TNF-alpha and IL-6) at the high dose. C1 [Kang, Jie; Thakali, Keshari M.] Univ Arkansas Med Sci, Dept Physiol & Biophys, USDA Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA. [Jensen, Gitte S.] NIS Labs, Klamath Falls, OR 97601 USA. [Wu, Xianli] Hershey Co, Food Res & Technol, Hershey, PA 17033 USA. RP Wu, XL (reprint author), Hershey Co, Food Res & Technol, 1025 Reese Ave, Hershey, PA 17033 USA. EM xwu@hersheys.com FU USDA [6251-51000-005-02S] FX This study was supported by USDA (6251-51000-005-02S). NR 33 TC 7 Z9 7 U1 4 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-9668 EI 1573-9104 J9 PLANT FOOD HUM NUTR JI Plant Food Hum. Nutr. PD MAR PY 2015 VL 70 IS 1 BP 56 EP 62 DI 10.1007/s11130-014-0461-6 PG 7 WC Plant Sciences; Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Plant Sciences; Chemistry; Food Science & Technology; Nutrition & Dietetics GA CC4PO UT WOS:000350335500009 PM 25535004 ER PT J AU Olanrewaju, HA Purswell, JL Maslin, WR Collier, SD Branton, SL AF Olanrewaju, H. A. Purswell, J. L. Maslin, W. R. Collier, S. D. Branton, S. L. TI Effects of color temperatures (kelvin) of LED bulbs on growth performance, carcass characteristics, and ocular development indices of broilers grown to heavy weights SO POULTRY SCIENCE LA English DT Article DE light-emitting diode; growth performance; carcass yield; stress; broilers ID MONOCHROMATIC LIGHT-SOURCES; CHICKENS; INTENSITY; ENLARGEMENT; VARIABLES; MELATONIN; QUALITY; GREEN; EYE AB Limited data are available for comparing light-emitting diode (LED) bulbs that are currently available in commercial broiler production facilities. We evaluated the effects of color temperatures (kelvin) of LED bulbs on growth performance, carcass characteristics, and ocular development indices of broilers grown to heavy weights (>3.0 kg). The experiment had a randomized complete block design. Four treatments consisted of 3 LED light bulbs (2,700 [warm LED]; 5,000 [cool LED 1]; and 5,000 K [cool LED 2]) and incandescent light (2,010 K [ICD], standard) from day zero to 56 d of age. A total of 960 Ross x Ross 708 day-old chicks were equally and randomly distributed into 16 environmentally controlled rooms at 50% RH (30 males and 30 females/room). Thus, each of the 4 treatments was represented by 4 rooms (4 replicates) per trial. Feed and water were provided ad libitum. All birds were fed the same diet. Ocular specimens were collected on d 42 for development and histopathologic examination. Blood samples were collected on d 21, 28, 42, and 56 to determine plasma corticosterone. On d 56, twenty birds from each room (10 males and 10 females) were processed to determine weights and yields. The BW and BW gain (BWG), live weight, and carcass weights of birds reared under cool LED 1 were different in comparison to birds reared under ICD (P < 0.05). However, feed intake (FI), feed conversion ratio (FCR), and mortality were not affected by treatments. The treatments did not affect fat, breast and tender weights, and yields. In addition, ocular development indices and plasma corticosterone concentrations were not affected by treatments, suggesting the LED light bulbs we evaluated did not compromise the welfare of the birds. It was concluded that cool LED 1 may be a better potential replacement light source in comparison to ICD on performance, but it may be equal when compared to other LED light sources examined in this study. C1 [Olanrewaju, H. A.; Purswell, J. L.; Collier, S. D.; Branton, S. L.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. [Maslin, W. R.] Mississippi State Univ, Coll Vet Med, Wise Ctr, Mississippi State, MS 39762 USA. RP Olanrewaju, HA (reprint author), ARS, USDA, Poultry Res Unit, POB 5367, Mississippi State, MS 39762 USA. EM hammed.olanrewaju@ars.usda.gov NR 32 TC 3 Z9 3 U1 2 U2 17 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAR PY 2015 VL 94 IS 3 BP 338 EP 344 DI 10.3382/ps/peu082 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC8ZQ UT WOS:000350657500002 PM 25667422 ER PT J AU Lu, Y Zhao, HY Liu, YQ Zhou, XP Wang, JY Liu, TT Beier, RC Hou, XL AF Lu, Yan Zhao, Hongyu Liu, Yuqi Zhou, Xuping Wang, Jinyuan Liu, Tiantian Beier, Ross C. Hou, Xiaolin TI Characterization of quinolone resistance in Salmonella enterica serovar Indiana from chickens in China SO POULTRY SCIENCE LA English DT Article DE Salmonella Indiana; quinolone resistance; aac (6')-Ib-cr; gyrA ID ESCHERICHIA-COLI; FLUOROQUINOLONE RESISTANCE; TYPHIMURIUM DT104; MODIFYING ENZYME; DETERMINANTS QNR; AAC(6')-IB-CR; PREVALENCE; GENES; QEPA AB The aim of this study was to characterize the quinolone resistance of Salmonella enterica serovar Indiana isolated from chickens in China. A total of 293 Salmonella strains were isolated from chicken farms and slaughterhouses in Shandong province of China, and 130 (44.4%) were characterized as Salmonella enterica Indiana (chicken farms, n = 52 strains; slaughter houses, n = 78 strains). All isolate serotypes were tested with the Kauffmann-White classification system and examined for susceptibility to the quinolones: nalidixic acid, enrofloxacin, norfloxacin, and ciprofloxacin. The resistance of the Salmonella Indiana strains to nalidixic acid, enrofloxacin, norfloxacin, and ciprofloxacin were 100, 73.1, 71.2, and 82.7%, and 100, 59.0, 79.5, and 80.2%, respectively. Selected quinolone resistant strains were evaluated for mutations in genes (gyrA, gyrB, parC, and marA) by DNA sequencing. The gyrA mutation was found in all isolates, the parC mutation was only found in some isolates, and the gyrB and marA mutations were not observed. Quinolone resistance was evaluated in the representative isolates by screening for the quinolone resistance determinants, qnrA, qnrB, qnrS, qepA, and aac (6')-Ib-cr using PCR technology. The quinolone resistance determinants in Salmonella, qnrA, qnrB, qnrS, and qepA were negative by PCR, but aac(6')-Ib-cr had high detection rates of 90.4 and 96.2% in chicken farms and slaughterhouses, respectively. Salmonella Indiana containing the gyrA mutation was prevalent in farms and slaughterhouses and possessed a high frequency of the quinolone resistance determinant aac(6')-Ib-cr. These bacteria may have originated from the same source. C1 [Lu, Yan; Liu, Yuqi; Zhou, Xuping; Hou, Xiaolin] Beijing Univ Agr, Coll Anim Sci & Technol, Beijing Key Lab Tradit Chinese Vet Med TCVM, Beijing 102206, Peoples R China. [Zhao, Hongyu] Chinese Acad Sci, Inst Biophys, State Key Lab Biomacromol, Beijing 100101, Peoples R China. [Wang, Jinyuan; Liu, Tiantian] Vet Hlth Epidem Prevent Stn, Beijing 101300, Peoples R China. [Beier, Ross C.] USDA ARS, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Liu, YQ (reprint author), Beijing Univ Agr, Coll Anim Sci & Technol, Beijing Key Lab Tradit Chinese Vet Med TCVM, Beijing 102206, Peoples R China. EM liuyuqi618@sina.com FU National Natural Science Foundation of China [31201949, 31172362]; Construction of Science Research Base of Beijing University of Agriculture [PXM2014_014207_000026] FX The authors have declared that no conflict of interest exists. This study was supported by the National Natural Science Foundation of China (31201949 and 31172362) and Construction of Science Research Base of Beijing University of Agriculture (PXM2014_014207_000026). NR 31 TC 1 Z9 1 U1 0 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAR PY 2015 VL 94 IS 3 BP 454 EP 460 DI 10.3382/ps/peu133 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC8ZQ UT WOS:000350657500017 PM 25701209 ER PT J AU Rothrock, MJ Ingram, KD Gamble, J Guard, J Cicconi-Hogan, KM Hinton, A Hiett, KL AF Rothrock, Michael J., Jr. Ingram, Kimberly D. Gamble, John Guard, Jean Cicconi-Hogan, Kellie M. Hinton, Arthur, Jr. Hiett, Kelli L. TI The characterization of Salmonella enterica serotypes isolated from the scalder tank water of a commercial poultry processing plant: Recovery of a multidrug-resistant Heidelberg strain SO POULTRY SCIENCE LA English DT Article DE S. Heidelberg; multidrug resistance; scalder water ID FIELD GEL-ELECTROPHORESIS; ANTIMICROBIAL RESISTANCE; ESCHERICHIA-COLI; UNITED-STATES; INTERNATIONAL SPREAD; ANIMAL ORIGIN; INFECTIONS; ENTERITIDIS; IDENTIFICATION; POPULATION AB The recent multistate outbreak of a multidrug-resistant (MDR) Salmonella Heidelberg strain from commercial poultry production highlights the need to better understand the reservoirs of these zoonotic pathogens within the commercial poultry production and processing environment. As part of a larger study looking at temporal changes in microbial communities within the major water tanks within a commercial processing facility, this paper identifies and characterizes Salmonella enterica isolated from the water in a final scalder tank at 3 times during a typical processing day: prior to the birds entering the tank (start), halfway through the processing day (mid), and after the final birds were scalded (end). Over 3 consecutive processing days, no Salmonella were recovered from start-of-day water samples, while a total of 56 Salmonella isolates were recovered from the mid-day and end-of-day scalder water samples. Traditional and newer PCR-based serotyping methods eventually identified these isolates as either group C3 S. Kentucky (n = 45) and group B S. Heidelberg (n = 11). While none of the S. Kentucky isolates possessed any resistances to the antimicrobials tested, all S. Heidelberg isolates were found to be multidrug resistant to 5 specific antimicrobials representing 3 antimicrobial classes. Due to the potential public health impact of S. Heidelberg and the recent nationwide poultry-associated outbreak of multidrug-resistant S. Heidelberg, future studies should focus on understanding the transmission and environmental growth dynamics of this serotype within the commercial poultry processing plant environment. C1 [Rothrock, Michael J., Jr.; Guard, Jean; Cicconi-Hogan, Kellie M.] USDA ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. [Ingram, Kimberly D.; Hinton, Arthur, Jr.; Hiett, Kelli L.] USDA ARS, Poultry Microbiol Safety & Proc Res Unit, Athens, GA 30605 USA. [Gamble, John] Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA. RP Rothrock, MJ (reprint author), USDA ARS, Egg Safety & Qual Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM michael.rothrock@ars.usda.gov FU Agricultural Research Service, USDA CRIS Project "Pathogen Reduction and Processing Parameters in Poultry Processing Systems" [6612-41420-017-00]; Agricultural Research Service, USDA CRIS Project "Molecular Approaches for the Characterization of Foodborne Pathogens in Poultry" [6612-32000-059-00] FX The authors would like to acknowledge the expert technical assistance of Nicole Bartenfeld, Kathy Tate, and Laura Lee-Rutherford for their assistance in sampling, as well as Latoya Wiggins and Tod Stewart for typing the isolates. These investigations were supported equally by the Agricultural Research Service, USDA CRIS Projects "Pathogen Reduction and Processing Parameters in Poultry Processing Systems" (6612-41420-017-00) and "Molecular Approaches for the Characterization of Foodborne Pathogens in Poultry" (6612-32000-059-00). NR 41 TC 4 Z9 4 U1 0 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAR PY 2015 VL 94 IS 3 BP 467 EP 472 DI 10.3382/ps/peu060 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC8ZQ UT WOS:000350657500019 PM 25681479 ER PT J AU Karcher, DM Jones, DR Abdo, Z Zhao, Y Shepherd, TA Xin, H AF Karcher, D. M. Jones, D. R. Abdo, Z. Zhao, Y. Shepherd, T. A. Xin, H. TI Impact of commercial housing systems and nutrient and energy intake on laying hen performance and egg quality parameters SO POULTRY SCIENCE LA English DT Article DE enriched colony cage; cage-free aviary; nutrition; conventional cage; egg quality ID DISTILLERS DRIED GRAINS; FURNISHED CAGES; CONVENTIONAL CAGES; DIETARY-PROTEIN; AVIARY SYSTEM; STRENGTH; WELFARE; CALCIUM; SOLUBLES; WHITE AB The US egg industry is exploring alternative housing systems for laying hens. However, limited published research related to cage-free aviary systems and enriched colony cages exists related to production, egg quality, and hen nutrition. The laying hen's nutritional requirements and resulting productivity are well established with the conventional cage system, but diminutive research is available in regards to alternative housing systems. The restrictions exist with limited availability of alternative housing systems in research settings and the considerable expense for increased bird numbers in a replicate due to alternative housing system design. Therefore, the objective of the current study was to evaluate the impact of nutrient and energy intake on production and egg quality parameters from laying hens housed at a commercial facility. Lohmann LSL laying hens were housed in three systems: enriched colony cage, cage-free aviary, and conventional cage at a single commercial facility. Daily production records were collected along with dietary changes during 15 production periods (28-d each). Eggs were analyzed for shell strength, shell thickness, Haugh unit, vitelline membrane properties, and egg solids each period. An analysis of covariance (ANCOVA) coupled with a principal components analysis (PCA) approach was utilized to assess the impact of nutritional changes on production parameters and monitored egg quality factors. The traits of hen-day production and mortality had a response only in the PCA 2 direction. This finds that as house temperature and Met intake increases, there is an inflection point at which hen-day egg production is negatively effected. Dietary changes more directly influenced shell parameters, vitelline membrane parameters, and egg total solids as opposed to laying hen housing system. Therefore, further research needs to be conducted in controlled research settings on laying hen nutrient and energy intake in the alternative housing systems and resulting impact on egg quality measures. C1 [Karcher, D. M.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Jones, D. R.] USDA, Agr Res Serv, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. [Abdo, Z.] USDA, Agr Res Serv, South Atlantic Area, Athens, GA 30605 USA. [Zhao, Y.; Shepherd, T. A.; Xin, H.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. RP Karcher, DM (reprint author), Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. EM dkarcher@msu.edu FU Coalition for a Sustainable Egg Supply (Kansas City, MO) FX Research support provided in part by a grant from the Coalition for a Sustainable Egg Supply (Kansas City, MO). NR 34 TC 7 Z9 8 U1 4 U2 29 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAR PY 2015 VL 94 IS 3 BP 485 EP 501 DI 10.3382/ps/peu078 PG 17 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC8ZQ UT WOS:000350657500022 PM 25630672 ER PT J AU Jones, DR Cox, NA Guard, J Fedorka-Cray, PJ Buhr, RJ Gast, RK Abdo, Z Rigsby, LL Plumblee, JR Karcher, DM Robison, CI Blatchford, RA Makagon, MM AF Jones, D. R. Cox, N. A. Guard, J. Fedorka-Cray, P. J. Buhr, R. J. Gast, R. K. Abdo, Z. Rigsby, L. L. Plumblee, J. R. Karcher, D. M. Robison, C. I. Blatchford, R. A. Makagon, M. M. TI Microbiological impact of three commercial laying hen housing systems SO POULTRY SCIENCE LA English DT Article DE hen housing; microbiology; egg; Salmonella; Campylobacter ID BACTERIAL EGGSHELL CONTAMINATION; FURNISHED CAGES; SALMONELLA-ENTERITIDIS; CONVENTIONAL CAGES; EGG MICROBIOLOGY; INTERNAL ORGANS; ENRICHED CAGES; SHELL EGGS; TABLE EGGS; CAMPYLOBACTER AB Hen housing for commercial egg production continues to be a societal and regulatory concern. Controlled studies have examined various aspects of egg safety, but a comprehensive assessment of commercial hen housing systems in the US has not been conducted. The current study is part of a holistic, multidisciplinary comparison of the diverse aspects of commercial conventional cage, enriched colony cage, and cage-free aviary housing systems and focuses on environmental and egg microbiology. Environmental swabs and eggshell pools were collected from all housing systems during 4 production periods. Total aerobes and coliforms were enumerated, and the prevalence of Salmonella and Campylobacter spp. was determined. Environmental aerobic and coliform counts were highest for aviary drag swabs (7.5 and 4.0 log cfu/mL, respectively) and enriched colony cage scratch pad swabs (6.8 and 3.8 log cfu/mL, respectively). Aviary floor and system wire shell pools had the greatest levels of aero-bic contamination for all eggshell pools (4.9 and 4.1 log cfu/mL, respectively). Hens from all housing systems were shedding Salmonella spp. (89-100% of manure belt scraper blade swabs). The dry belt litter removal processes for all housing systems appear to affect Campylobacter spp. detection (0-41% of manure belt scraper blade swabs) considering detection of Campylobacter spp. was much higher for other environmental samples. Aviary forage area drag swabs were 100% contaminated with Campylobacter spp., whereas enriched colony cage scratch pads had a 93% positive rate. There were no differences in pathogen detection in the shell pools from the 3 housing systems. Results indicate egg safety is enhanced when hens in alternative housing systems use nest boxes. Additionally, current outcomes indicate the use of scratch pads in hen housing systems needs to be more thoroughly investigated for effects on hen health and egg safety. C1 [Jones, D. R.; Cox, N. A.; Guard, J.; Fedorka-Cray, P. J.; Buhr, R. J.; Gast, R. K.; Abdo, Z.; Rigsby, L. L.; Plumblee, J. R.] ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. [Karcher, D. M.; Robison, C. I.; Makagon, M. M.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Blatchford, R. A.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. RP Jones, DR (reprint author), ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. EM Deana.Jones@ars.usda.gov FU Coalition for a Sustainable Egg Supply (Kansas City, MO) FX Research support provided in part by a grant from the Coalition for a Sustainable Egg Supply (Kansas City, MO). NR 27 TC 3 Z9 3 U1 2 U2 21 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAR PY 2015 VL 94 IS 3 BP 544 EP 551 DI 10.3382/ps/peu010 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC8ZQ UT WOS:000350657500027 PM 25480737 ER PT J AU Huang, HL Sun, JH Mccoy, JA Zhong, HY Fletcher, EJ Harnly, J Chen, P AF Huang, Huilian Sun, Jianghao Mccoy, Joe-Ann Zhong, Haiyan Fletcher, Edward J. Harnly, James Chen, Pei TI Use of flow injection mass spectrometric fingerprinting and chemometrics for differentiation of three black cohosh species SO SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY LA English DT Article DE Black cohosh; Mass spectrometer; Fingerprinting; PCA ID PERFORMANCE LIQUID-CHROMATOGRAPHY; CIMICIFUGA-RACEMOSA; PRODUCTS; ACTAEA; IDENTIFICATION; GLYCOSIDES AB Flow injection mass spectrometry (FIMS) was used to provide chemical fingerprints of black cohosh (Actaea racemosa L.) in a manner of minutes by omitting the separation step. This method has proven to be a powerful tool for botanical authentication and in this study it was used to distinguish between three Actaea species prior to a more detailed chemical analysis using ultra high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Black cohosh has become increasingly popular as a dietary supplement in the United States for the treatment of symptoms related to menopause. However, it has been known to be adulterated with the Asian Actaea dahurica (Turcz. ex Fisch. & C.A.Mey.) Franch. species (syn. Cimicifuga dahurica (Turcz.) Maxim). Existing methods for identification of black cohosh and differentiation of Actaea species are usually lengthy, laborious, and lack robustness, often based on the comparison of a few pre-selected components. Chemical fingerprints were obtained for 77 black cohosh samples and their related species using FIMS in the negative ion mode. The analysis time for each sample was less than 2 mm, All data were processed using principal component analysis (PCA). FIMS fingerprints could readily differentiate all three species. Representative samples from each of the three species were further examined using UHPLC-MS to provide detailed profiles of the chemical differences between the three species and were compared to the PCA loadings. This study demonstrates a simple, fast, and easy analytical method that can be used to differentiate A. racemosa, Actaea podocarpa, and A. dahurica. (C) 2014 Published by Elsevier B.V. C1 [Huang, Huilian; Sun, Jianghao; Harnly, James; Chen, Pei] USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. [Huang, Huilian] Jiangxi Univ Tradit Chinese Med, TCM, Key Lab Modern Preparat, Minist Educ, Nanchang, Jiangxi, Peoples R China. [Mccoy, Joe-Ann] North Carolina Arboretum Germplasrn Repository, Asheville, NC USA. [Zhong, Haiyan] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha, Hunan, Peoples R China. [Fletcher, Edward J.] Strategic Sourcing Inc, Banner Elk, NC 28604 USA. RP Harnly, J (reprint author), USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. EM harnly.james@ars.usda.gov; pei.chen@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture [4080-52000-063-00D]; Office of Dietary Supplements at the National Institutes of Health (IIAA) [AOD12026-001-01002]; University's Young Teachers' Development Project of Jiangxi Province, China [2012-132] FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture (Project #4080-52000-063-00D), an Interagency Agreement with the Office of Dietary Supplements at the National Institutes of Health (IIAA # AOD12026-001-01002), and the University's Young Teachers' Development Project of Jiangxi Province (2012-132), China. NR 30 TC 4 Z9 4 U1 2 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0584-8547 J9 SPECTROCHIM ACTA B JI Spectroc. Acta Pt. B-Atom. Spectr. PD MAR 1 PY 2015 VL 105 SI SI BP 121 EP 129 DI 10.1016/j.sab.2014.10.005 PG 9 WC Spectroscopy SC Spectroscopy GA CC8IL UT WOS:000350611600020 ER PT J AU Nelson, MI Vincent, AL AF Nelson, Martha I. Vincent, Amy L. TI Reverse zoonosis of influenza to swine: new perspectives on the human-animal interface SO TRENDS IN MICROBIOLOGY LA English DT Review DE influenza A virus; swine; pandemic; evolution; human-animal interface ID PANDEMIC H1N1 2009; COMPLETE GENOME SEQUENCE; NORTH-AMERICAN SWINE; A VIRUSES; UNITED-STATES; GENETIC-CHARACTERIZATION; SOUTHERN CHINA; RESPIRATORY-DISEASE; EQUINE INFLUENZA; PIG-POPULATION AB The origins of the 2009 influenza A (H1N1) pandemic in swine are unknown, highlighting gaps in our understanding of influenza A virus (IAV) ecology and evolution. We review how recently strengthened influenza virus surveillance in pigs has revealed that influenza virus transmission from humans to swine is far more frequent than swine-to-human zoonosis, and is central in seeding swine globally with new viral diversity. The scale of global human-to-swine transmission represents the largest 'reverse zoonosis' of a pathogen documented to date. Overcoming the bias towards perceiving swine as sources of human viruses, rather than recipients, is key to understanding how the bidirectional nature of the human-animal interface produces influenza threats to both hosts. C1 [Nelson, Martha I.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Vincent, Amy L.] USDA ARS, Natl Anim Dis Ctr, Virus & Prion Res Unit, Ames, IA 50010 USA. RP Nelson, MI (reprint author), NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. EM nelsonma@mail.nih.gov FU Office of Global Affairs at the US Department of Health and Human Services (DHHS); USDA-ARS FX We would like to thank National Institutes of Health (NIH) intern Adaeze Ezeofor for her contributions to the organization and identification of the many journal references cited in this manuscript. We are also grateful to Drs Cecile Viboud (NIH) and Edward C. Holmes (University of Sydney) for their insightful comments on earlier drafts of this manuscript. This research was conducted within the context of the Multinational Influenza Seasonal Mortality Study (MISMS) (http://www.origem.info/misms/), an ongoing international collaborative effort to understand influenza epidemiology and evolution, led by the Fogarty International Center, NIH, with funding from the Office of Global Affairs at the US Department of Health and Human Services (DHHS) (M.I.N.). A.V. is funded by USDA-ARS. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 135 TC 28 Z9 30 U1 4 U2 41 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0966-842X EI 1878-4380 J9 TRENDS MICROBIOL JI Trends Microbiol. PD MAR PY 2015 VL 23 IS 3 BP 142 EP 153 DI 10.1016/j.tim.2014.12.002 PG 12 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA CD2TZ UT WOS:000350933200005 PM 25564096 ER PT J AU Zielinski, WJ Moriarty, KM Baldwin, J Kirk, TA Slauson, KM Rustigian-Romsos, HL Spencer, WD AF Zielinski, William J. Moriarty, Katie M. Baldwin, Jim Kirk, Thomas A. Slauson, Keith M. Rustigian-Romsos, Heather L. Spencer, Wayne D. TI Effects of season on occupancy and implications for habitat modeling: the Pacific marten Martes caurina SO WILDLIFE BIOLOGY LA English DT Article ID AMERICAN MARTEN; HOME-RANGE; DETECTION PROBABILITIES; CALIFORNIA; FOREST; ABUNDANCE; SELECTION; RATES; BIAS AB Season affects many characteristics of populations and, as a result, the interpretations of surveys conducted at different seasons. We explored seasonal variation in occupancy using data from four studies on the Pacific marten Martes caurina. Detection surveys were conducted during winter and summer using either cameras or track stations. We conducted a 'multiple location, paired season' analysis using data from all four study areas and a 'multiple season' analysis using seasonally replicated occupancy data collected at one of the areas. In the former analysis, summer occupancy estimates were significantly lower than winter and per visit probabilities of detection were indistinguishable between seasons. The probabilities of detection for the complete survey protocol were high (0.83 summer, 0.95 winter). Where summer and winter surveys were replicated, probability of occupancy was 5 times higher in winter (0.52) than summer (0.09). We considered the effect of seasonal variation in occupancy on the habitat models developed using summer and winter survey data. Using the same habitat suitability threshold (0.5), the weighted average of winter models predicted significantly more suitable habitat than summer models. The habitat predicted by the summer model was at higher elevation, and was distributed among more, and smaller, patches of habitat than the model developed using winter data. We expect a similar magnitude of differences if summer or winter data were used to monitor occupancy. The higher occupancy in winter is probably due to the abundance of young animals detected during dispersal. Summer survey results reflect the distribution of territory-holding adults, thus these surveys may reliably detect breeding individuals and represent reproductive habitat. The implications of season on the interpretation of survey results, and corresponding habitat models and monitoring programs, provide a challenge to managers that make decisions about habitat management for martens, and other species with disparate occupancy among seasons. C1 [Zielinski, William J.; Slauson, Keith M.] USDA Forest Serv, Pacific Southwest Res Stn, Arcata, CA 95521 USA. [Moriarty, Katie M.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Baldwin, Jim] USDA Forest Serv, Pacific Southwest Res Stn, Berkeley, CA 94701 USA. [Kirk, Thomas A.] USDA Forest Serv, Lassen Natl Forest, Susanville, CA 96130 USA. [Rustigian-Romsos, Heather L.] Conservat Biol Inst, Corvallis, OR 97333 USA. [Spencer, Wayne D.] Conservat Biol Inst, San Diego, CA 92116 USA. RP Zielinski, WJ (reprint author), USDA Forest Serv, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU Lassen National Forest; Tahoe National Forest; Lake Tahoe Basin Management Unit; Sierra National Forest; Pacific Southwest Region; Pacific Southwest Research Station of the USDA Forest Service; Herger Feinstein Quincy Library Group FX We thank T. Frolli and M. Williams, of the Lassen National Forest, for support and C. Epps, Oregon State University, for facilitating funding. We appreciate the support of the Lassen National Forest, Tahoe National Forest, Lake Tahoe Basin Management Unit and the Sierra National Forest and the Pacific Southwest Region and the Pacific Southwest Research Station of the USDA Forest Service and the Herger Feinstein Quincy Library Group. Discussions with Lassen National Forest biologists and J. Perrine were instrumental in developing this work. We thank the following for collecting and summarizing field data: R. Beane, T. Dodson, K. Gaudet, C. Heard, M. Delheimer, C. Kinnard, R. Kussow, P. Lieske, M. Linnell, K. Mansfield, B. Marckmann, M. Matsen, K. Moughamer, W. Owens, K. Phillips, A. Rogers, E. Shea, P. Squires, R. Schlexer and A. Stutz. We thank B. Noon and the editor for suggestions that improved the manuscript considerably. NR 41 TC 2 Z9 2 U1 3 U2 18 PU WILDLIFE BIOLOGY PI RONDE PA C/O JAN BERTELSEN, GRENAAVEJ 14, KALO, DK-8410 RONDE, DENMARK SN 0909-6396 EI 1903-220X J9 WILDLIFE BIOL JI Wildlife Biol. PD MAR PY 2015 VL 21 IS 2 BP 56 EP 67 DI 10.2981/wlb.00077 PG 12 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CC6DM UT WOS:000350455100001 ER PT J AU Connor, EE AF Connor, E. E. TI Invited review: Improving feed efficiency in dairy production: challenges and possibilities SO ANIMAL LA English DT Article DE dairy cow; feed efficiency; residual feed intake ID GREENHOUSE-GAS EMISSIONS; HOLSTEIN-FRIESIAN COWS; COPY NUMBER VARIATION; ANGUS BEEF-CATTLE; GENETIC-PARAMETERS; METHANE PRODUCTION; GENOMIC SELECTION; ENERGY-BALANCE; PRODUCTION TRAITS; MILK-PRODUCTION AB Despite substantial advances in milk production efficiency of dairy cattle over the last 50 years, rising feed costs remain a significant threat to producer profitability. There also is a greater emphasis being placed on reducing the negative impacts of dairy production on the environment; thus means to lower greenhouse gas (GHG) emissions and nutrient losses to the environment associated with cattle production are being sought. Improving feed efficiency among dairy cattle herds offers an opportunity to address both of these issues for the dairy industry. However, the best means to assess feed efficiency and make genetic progress in efficiency-related traits among lactating cows without negatively impacting other economically important traits is not entirely obvious. In this review, multiple measurements of feed efficiency for lactating cows are described, as well as the heritability of the traits and their genetic and phenotypic correlations with other production traits. The measure of feed efficiency, residual feed intake is discussed in detail in terms of the benefits for its selection, how it could be assessed in large commercial populations, as well as biological mechanisms contributing to its variation among cows, as it has become a commonly used method to estimate efficiency in the recent scientific literature. C1 USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Connor, EE (reprint author), USDA ARS, Anim Genom & Improvement Lab, 10300 Baltimore Ave,Bldg 306,BARC East, Beltsville, MD 20705 USA. EM erin.connor@ars.usda.gov NR 102 TC 7 Z9 7 U1 5 U2 60 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD MAR PY 2015 VL 9 IS 3 BP 395 EP 408 DI 10.1017/S1751731114002997 PG 14 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA CC0PN UT WOS:000350038100004 PM 25482927 ER PT J AU Lammers, PJ Kerr, BJ Honeyman, MS AF Lammers, P. J. Kerr, B. J. Honeyman, M. S. TI Biofuel co-products as swine feed ingredients: Combining corn distillers dried grains with solubles (DDGS) and crude glycerin SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Corn DDGS; Crude glycerin; Fatty acid profile; Performance; Pig; Pork adipose ID CARCASS FAT QUALITY; GROWER-FINISHER PIGS; GROWING PIGS; PORK QUALITY; DIETS; PERFORMANCE; DIGESTIBILITY; OIL AB The objective of this study was to examine the effects of combining corn DDGS with crude glycerin on growth performance and carcass traits to determine if a high level of crude glycerin supplementation could counteract the impact of corn DDGS on fatty acid profile of pork adipose. The experimental design was a 3 x 2 factorial arrangement of treatments with three levels of corn DDGS (0,150, or 250 g/kg diet) and two levels of crude glycerin (0 or 100 g/kg diet). Pigs were fed one of six experimental diets over a 3-phase feeding,program for 84 d with each diet within phase formulated to be equal in metabolizable energy (ME) and standardized heal digestible lysine (SID Lys). Pigs and feeders were weighed every 14 d to determine average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency (G:F). On d-84, pigs were weighed and scanned using real-time ultrasound to obtain fat depth and longissimus dorsi muscle (LM) area. Pigs were then harvested at a commercial abattoir and a sample of adipose was collected from the jowl of each pig. Because there was no interaction between level of corn DDGS and crude glycerin fed, only main effects are presented. Pig performance and carcass characteristics were not affected by dietary treatment (P >= 0.05). Differences in fatty acid composition were present due to dietary treatment. Increasing the level of corn DDGS reduced concentration of both saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) and increased the concentration of polyunsaturated fatty acids (PUFA) in pork jowl adipose (P<0.01). Pigs fed 100 g crude glycerin/kg diet had higher concentrations of MUFA and lower concentrations of PUFA (P<0.05). Diets containing up to 250 g corn DDGS/kg and 100 g crude glycerin/kg support growth of finishing pigs. However, increasing dietary levels of corn DDGS increased the concentration of unsaturated fatty acids in pork jowl adipose and this was not ameliorated by feeding 100 g/kg crude glycerin. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lammers, P. J.] IIT, Dept Agr, Normal, IL 61790 USA. [Kerr, B. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Honeyman, M. S.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. RP Lammers, PJ (reprint author), IIT, Dept Agr, Normal, IL 61790 USA. EM pjlamme@ilstu.edu FU Hatch Act; USDA Special Grant; USDA-ARS; Iowa Grain Quality Initiative; Agricultural Marketing Resource Center; Leopold Center for Sustainable Agriculture funds; State of Iowa FX This project was supported by Hatch Act, State of Iowa, USDA Special Grant, USDA-ARS, Iowa Grain Quality Initiative, Agricultural Marketing Resource Center, and Leopold Center for Sustainable Agriculture funds. The authors gratefully acknowledge Hawkeye Gold, LLC, Ames, IA; Central Iowa Energy, Newton, IA; and DSM Nutritional Products, Parsippany, NJ for providing test ingredients used in this research. The authors also gratefully acknowledge the assistance of the staff of the Iowa State University Swine Nutrition Research Farm, A. Penner for animal care, data collection, and statistical analyses, and Dr. Dong Ahn Iowa State University Ames, IA for laboratory assistance. Mention of trade names of commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendations or endorsement by Illinois State University, Iowa State University, or the USDA. The USDA is an equal opportunity employer. NR 19 TC 1 Z9 1 U1 1 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 EI 1873-2216 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD MAR PY 2015 VL 201 BP 110 EP 114 DI 10.1016/j.anifeedsci.2014.12.013 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC6ZD UT WOS:000350516600013 ER PT J AU Zalapa, JE Bougie, TC Bougie, TA Schlautman, BJ Wiesman, E Guzman, A Fajardo, DA Steffan, S Smith, T AF Zalapa, J. E. Bougie, T. C. Bougie, T. A. Schlautman, B. J. Wiesman, E. Guzman, A. Fajardo, D. A. Steffan, S. Smith, T. TI Clonal diversity and genetic differentiation revealed by SSR markers in wild Vaccinium macrocarpon and Vaccinium oxycoccos SO ANNALS OF APPLIED BIOLOGY LA English DT Article DE clonal diversity; Cranberry; genetic differentiation; hybridisation; microsatellite markers; ploidy ID AMERICAN CRANBERRY; MICROSATELLITE MARKERS; ERICACEAE; POPULATIONS; DISTANCE; RAPDS; LOCI; AIT.; EST; PCR AB American cranberry (Vaccinium macrocarpon) is a perennial, woody plant species, native to North American bogs and wetlands. Cranberries represent one of the few agriculturally important native plants in which wild gene pools are still readily available within the undeveloped wetlands of the northern US and Canada. Earlier studies have reported low genetic variation in V. macrocarpon at the species and population level. However, in this study, we characterised 229 individuals of wild V. macrocarpon and V. oxycoccos (small cranberry) from Wisconsin and 22 accessions using microsatellite markers and observed substantial genetic variation and differentiation within and among populations and species. While V. macrocarpon was analysed using 108 alleles from 11 microsatellite loci revealing 42 unique genotypes, V. oxycoccos was analysed using 156 alleles from eight loci revealing 28 unique genotypes. There were a total of 182 alleles found in both species combined with 156 of those alleles present in V. oxycoccos and 84 alleles found in V. macrocarpon. All eight loci possessed species-specific alleles with V. oxycoccos possessing 98 private alleles versus 26 private alleles found V. macrocarpon, and 58 alleles were found in common between both species. Our data will be valuable not only for future wild cranberry diversity and population genetics research, but for other cranberry breeding and genetics studies. C1 [Zalapa, J. E.; Wiesman, E.; Steffan, S.] ARS, USDA, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Bougie, T. C.; Bougie, T. A.; Schlautman, B. J.; Guzman, A.; Fajardo, D. A.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Smith, T.] Agr & Agri Food Canada, Ottawa, ON K1A 0C6, Canada. RP Zalapa, JE (reprint author), ARS, USDA, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM jezalapa@wisc.edu OI Smith, Tyler/0000-0001-7683-2653 FU National Science Foundation [DBI-1228280]; Wisconsin State Cranberry Growers Association; Cranberry Institute; USDA-ARS [3655-21220-001-00] FX Rom. 8:31-39. We thank Nicholi Vorsa, James Polashock, and Jennifer Johnson-Cicalese for providing the diploid and tetraploid V. oxycoccos controls. The authors thank Massimo Iorizzo for his helpful comments on this manuscript. This research was supported by funds from the National Science Foundation (DBI-1228280), Wisconsin State Cranberry Growers Association, Cranberry Institute, and USDA-ARS (project no. 3655-21220-001-00) provided to J.E.Z. and S.A.S. NR 42 TC 4 Z9 5 U1 2 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0003-4746 EI 1744-7348 J9 ANN APPL BIOL JI Ann. Appl. Biol. PD MAR PY 2015 VL 166 IS 2 BP 196 EP 207 DI 10.1111/aab.12173 PG 12 WC Agriculture, Multidisciplinary SC Agriculture GA CC4XA UT WOS:000350357300003 ER PT J AU Patel, JR Yossa, I Macarisin, D Millner, P AF Patel, Jitendra R. Yossa, Irene Macarisin, Dumitru Millner, Patricia TI Physical Covering for Control of Escherichia coli O157:H7 and Salmonella spp. in Static and Windrow Composting Processes SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ZOONOTIC AGENTS; SOLID FRACTION; BOVINE MANURE; STRAW MIXTURE; ON-FARM; WASTES; FATE; PATHOGENS; O157-H7; SYSTEMS AB This study investigated the effect of a 30-cm covering of finished compost (FC) on survival of Escherichia coli O157:H7 and Salmonella spp. in active static and windrow composting systems. Feedstocks inoculated with E. coli O157:H7 (7.41 log CFU/g) and Salmonella (6.46 log CFU/g) were placed in biosentry tubes (7.5-cm diameter, 30-cm height) at three locations:(i and ii) two opposing sides at the interface between the FC cover layer (where present) and the feedstock material (each positioned approximately 10 cm below the pile's surface) and (iii) an internal location (top) (approximately 30 cm below the surface). On specific sampling days, surviving populations of inoculated E. coli O157:H7 and Salmonella, generic E. coli, and coliforms in compost samples were determined. Salmonella spp. were reduced significantly within 24 h in windrow piles and were below the detection limit after 3 and 7 days at internal locations of windrow and static piles containing FC covering, respectively. Likewise, E. coli O157:H7 was undetectable after 1 day in windrow piles covered with finished compost. Use of FC as a covering layer significantly increased the number of days that temperatures in the windrows remained >= 55 degrees C at all locations and in static piles at internal locations. These time-temperature exposures resulted in rapid reduction of inoculated pathogens, and the rate of bacterial reduction was rapid in windrow piles. The sample location significantly influenced the survival of these pathogens at internal locations compared to that at interface locations of piles. Finished compost covering of compost piles aids in the reduction of pathogens during the composting process. C1 [Patel, Jitendra R.; Yossa, Irene; Macarisin, Dumitru; Millner, Patricia] USDA ARS, Beltsville Area Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Patel, JR (reprint author), USDA ARS, Beltsville Area Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM jitu.patel@ars.usda.gov FU Center for Produce Safety grant [SCB10055] FX This work was supported by the Center for Produce Safety grant (SCB10055). NR 40 TC 1 Z9 1 U1 3 U2 25 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2015 VL 81 IS 6 BP 2063 EP 2074 DI 10.1128/AEM.04002-14 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CC7NM UT WOS:000350554800021 PM 25576620 ER PT J AU Ramdas, KEF Lyashchenko, KP Greenwald, R Robbe-Austerman, S McManis, C Waters, WR AF Ramdas, Kira E. F. Lyashchenko, Konstantin P. Greenwald, Rena Robbe-Austerman, Suelee McManis, Cynthia Waters, W. Ray TI Mycobacterium bovis Infection in Humans and Cats in Same Household, Texas, USA, 2012 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID TUBERCULOSIS; ANTIGENS AB Mycobacterium bovis infection of cats is exceedingly rare in regions where bovine tuberculosis is not endemic. We describe the diagnosis and clinical management of pulmonary M. bovis infection in 2 indoor-housed cats and their association with at least 1 M. bovis-infected human in Texas, USA, in September 2012. C1 [Ramdas, Kira E. F.; McManis, Cynthia] Just Cats Vet Serv, The Woodlands, TX 77380 USA. [Lyashchenko, Konstantin P.; Greenwald, Rena] Chembio Diagnost Syst Inc, Medford, NY USA. [Robbe-Austerman, Suelee] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Waters, W. Ray] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Ramdas, KEF (reprint author), Just Cats Vet Serv, 1015 Evergreen Circle, The Woodlands, TX 77380 USA. EM kira.ramdas@gmail.com NR 15 TC 0 Z9 0 U1 0 U2 10 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD MAR PY 2015 VL 21 IS 3 BP 480 EP 483 DI 10.3201/eid2103.140715 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CC3SI UT WOS:000350269300017 PM 25695666 ER PT J AU Butler, WH Monroe, A McCaffrey, S AF Butler, William H. Monroe, Ashley McCaffrey, Sarah TI Collaborative Implementation for Ecological Restoration on US Public Lands: Implications for Legal Context, Accountability, and Adaptive Management SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Collaborative implementation; Ecological restoration; Public lands management; Accountability; Adaptive management ID GOVERNANCE; SCALE; SYSTEMS AB The Collaborative Forest Landscape Restoration Program (CFLRP), established in 2009, encourages collaborative landscape scale ecosystem restoration efforts on United States Forest Service (USFS) lands. Although the USFS employees have experience engaging in collaborative planning, CFLRP requires collaboration in implementation, a domain where little prior experience can be drawn on for guidance. The purpose of this research is to identify the ways in which CFLRP's collaborative participants and agency personnel conceptualize how stakeholders can contribute to implementation on landscape scale restoration projects, and to build theory on dynamics of collaborative implementation in environmental management. This research uses a grounded theory methodology to explore collaborative implementation from the perspectives and experiences of participants in landscapes selected as part of the CFLRP in 2010. Interviewees characterized collaborative implementation as encompassing three different types of activities: prioritization, enhancing treatments, and multiparty monitoring. The paper describes examples of activities in each of these categories and then identifies ways in which collaborative implementation in the context of CFLRP (1) is both hindered and enabled by overlapping legal mandates about agency collaboration, (2) creates opportunities for expanded accountability through informal and relational means, and, (3) creates feedback loops at multiple temporal and spatial scales through which monitoring information, prioritization, and implementation actions shape restoration work both within and across projects throughout the landscape creating more robust opportunities for adaptive management. C1 [Butler, William H.; Monroe, Ashley] Florida State Univ, Tallahassee, FL 32306 USA. [McCaffrey, Sarah] US Forest Serv, No Res Stn, Evanston, IL USA. RP Butler, WH (reprint author), Florida State Univ, Tallahassee, FL 32306 USA. EM wbutler@fsu.edu FU US Forest Service (USFS) Northern Research Station; Nature Conservancy FX This research was funded by the US Forest Service (USFS) Northern Research Station and further supported by a travel grant from The Nature Conservancy. The authors wish to thank the participants in this study whose time and thoughtful reflections were invaluable to this research. Also, we are grateful to three reviewers as well as Drs. Robert Deyle and Rachel Christensen whose insightful suggestions contributed significantly to improving the quality of this work. NR 48 TC 6 Z9 6 U1 4 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD MAR PY 2015 VL 55 IS 3 BP 564 EP 577 DI 10.1007/s00267-014-0430-8 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA CC5AI UT WOS:000350368300004 PM 25549996 ER PT J AU Kim, K Labbe, N Warren, JM Elder, T Rials, TG AF Kim, Keonhee Labbe, Nicole Warren, Jeffrey M. Elder, Thomas Rials, Timothy G. TI Chemical and anatomical changes in Liquidambar styraciflua L. xylem after long term exposure to elevated CO2 SO ENVIRONMENTAL POLLUTION LA English DT Article DE Free air CO2 enrichment; Sweetgum; Chemical composition; Hydraulic conductivity; PCA ID STEM WOOD PROPERTIES; CARBON-DIOXIDE CONCENTRATION; MATURE NORWAY SPRUCE; ATMOSPHERIC CO2; CO2-ENRICHED ATMOSPHERE; NITROGEN-FERTILIZATION; NUTRIENT AVAILABILITY; TROPOSPHERIC O-3; DECIDUOUS FOREST; WATER-FLOW AB The anatomical and chemical characteristics of sweetgum were studied after 11 years of elevated CO2 (544 ppm, ambient at 391 ppm) exposure. Anatomically, branch xylem cells were larger for elevated CO2 trees, and the cell wall thickness was thinner. Chemically, elevated CO2 exposure did not impact the structural components of the stem wood, but non-structural components were significantly affected. Principal component analysis (PCA) was employed to detect differences between the CO2 treatments by considering numerous structural and chemical variables, as well as tree size, and data from previously published sources (i.e., root biomass, production and turnover). The PCA results indicated a clear separation between trees exposed to ambient and elevated CO2 conditions. Correlation loadings plots of the PCA revealed that stem structural components, ash, Ca, Mg, total phenolics, root biomass, production and turnover were the major responses that contribute to the separation between the elevated and ambient CO2 treated trees. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Kim, Keonhee; Labbe, Nicole; Rials, Timothy G.] Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN 37996 USA. [Warren, Jeffrey M.] Oak Ridge Natl Lab, Climate Change Sci Inst, Oak Ridge, TN USA. [Warren, Jeffrey M.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Elder, Thomas] US Forest Serv, USDA, Southern Res Stn, Pineville, LA USA. RP Labbe, N (reprint author), Univ Tennessee, Ctr Renewable Carbon, 2506 Jacob Dr, Knoxville, TN 37996 USA. EM nlabbe@utk.edu RI Warren, Jeffrey/B-9375-2012 OI Warren, Jeffrey/0000-0002-0680-4697 FU U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC05-000R22725]; U.S. Department of Agriculture [2010-34158-20930] FX This study was supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, under contract DE-AC05-000R22725, and U.S. Department of Agriculture, award number 2010-34158-20930. NR 49 TC 2 Z9 3 U1 2 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD MAR PY 2015 VL 198 BP 179 EP 185 DI 10.1016/j.envpol.2015.01.006 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CC2RF UT WOS:000350191600023 PM 25603157 ER PT J AU Meigs, GW Kennedy, RE Gray, AN Gregory, MJ AF Meigs, Garrett W. Kennedy, Robert E. Gray, Andrew N. Gregory, Matthew J. TI Spatiotemporal dynamics of recent mountain pine beetle and western spruce budworm outbreaks across the Pacific Northwest Region, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bark beetle; Change detection; Defoliator; Forest disturbance; Landsat time series; Tree mortality ID TREE MORTALITY; BARK BEETLE; LANDSAT IMAGERY; FOREST STRUCTURE; UNITED-STATES; DISTURBANCE HISTORY; TEMPORAL PATTERNS; DETECTING TRENDS; CLIMATIC-CHANGE; OREGON AB Across the western US, the two most prevalent native forest insect pests are mountain pine beetle (MPB; Dendroctottus ponderosae; a bark beetle) and western spruce budworm (WSB; Choristoneura freemani; a defoliator). MPB outbreaks have received more forest management attention than WSB outbreaks, but studies to date have not compared their cumulative mortality impacts in an integrated, regional framework. The objectives of this study are to: (1) map tree mortality associated with MPB and WSB outbreaks by integrating forest health aerial detection surveys (ADS; 1970-2012), Landsat time series (1984-2012), and multi-date forest inventory data; (2) compare the timing, extent, and cumulative impacts of recent MPB and WSB outbreaks across forested ecoregions of the US Pacific Northwest Region (PNW; Oregon and Washington). Our Landsat-based insect atlas facilitates comparisons across space, time, and insect agents that have not been possible to date, complementing existing ADS maps in three important ways. The new maps (1) capture variation of insect impacts within ADS polygons at a finer spatial resolution (30 m), substantially reducing estimated insect extent; (2) provide consistent estimates of change for multiple agents, particularly long-duration changes; (3) quantify change in terms of field-measured tree mortality (dead basal area). Despite high variation across the study region, spatiotemporal patterns are evident in both the aerial survey- and Landsat-based maps of insect activity. MPB outbreaks occurred in two phases first during the 1970s and 1980s in eastern and central Oregon and then more synchronously during the 2000s throughout dry interior conifer forests of the PNW. Reflecting differences in habitat susceptibility and epidemiology, WSB outbreaks exhibited early activity in northern Washington and an apparent spread from the eastern to central PNVV during the 1980s, returning to northern Washington during the 1990s and 2000s. At ecoregional and regional scales, WSB outbreaks have exceeded MPB outbreaks in extent as well as total tree mortality, suggesting that ongoing studies should account for both bark beetles and defoliators. Given projected increases of insect and fire activity in western forests, the accurate assessment and monitoring of these disturbances will be crucial for sustainable ecosystem management. (C) 2014 Elsevier B.V. All rights reserved. C1 [Meigs, Garrett W.; Gregory, Matthew J.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Kennedy, Robert E.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Gray, Andrew N.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Meigs, GW (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. EM gmeigs@gmail.com FU NASA Headquarters under the NASA Earth and Space Science Fellowship Program Grant [NNX11AM01H]; USDA Forest Service, Forest Health Monitoring (FHM) Evaluation Monitoring [WC-F-09-2]; USDA Forest Service Northwest Forest Plan Effectiveness Monitoring Program; North American Carbon Program through grants from NASA's Terrestrial Ecology, Carbon Cycle Science, and Applied Sciences Programs; NASA New Investigator Program; Office of Science (BER) of the U.S. Department of Energy; Inventory and Monitoring networks of the National Park Service: Southwest Alaska, Sierra Nevada, Northern Colorado Plateau, and Southern Colorado Plateau FX This research was supported in part by NASA Headquarters under the NASA Earth and Space Science Fellowship Program Grant NNX11AM01H - and the USDA Forest Service, Forest Health Monitoring (FHM) Evaluation Monitoring - Grant No. WC-F-09-2. We acknowledge Justin Braaten, Kevin Briggs, John Campbell, Julie Johnson, Helen Maffei, Trevor McWilliams, Andrew Merschel, Ariel Muldoon, Matthew Reilly, Heather Roberts, Keith Sprengel, Elizabeth Willhite, Zhiqiang Yang, and Harold Zald for help with data access, analysis, and interpretation. We thank G. Kohler for help with insect taxonomy and the many PNW staff and contractors for collecting and compiling the aerial survey and forest inventory databases. We are grateful to Tiffany Garcia, James Johnston, Jono Meigs, David Shaw, and two anonymous reviewers for their insightful comments. The development and testing of the LandTrendr algorithms reported in this paper were made possible with the support of the USDA Forest Service Northwest Forest Plan Effectiveness Monitoring Program, the North American Carbon Program through grants from NASA's Terrestrial Ecology, Carbon Cycle Science, and Applied Sciences Programs, the NASA New Investigator Program, the Office of Science (BER) of the U.S. Department of Energy, and the following Inventory and Monitoring networks of the National Park Service: Southwest Alaska, Sierra Nevada, Northern Colorado Plateau, and Southern Colorado Plateau. NR 46 TC 15 Z9 15 U1 3 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2015 VL 339 BP 71 EP 86 DI 10.1016/j.foreco.2014.11.030 PG 16 WC Forestry SC Forestry GA CC6ZK UT WOS:000350517300007 ER PT J AU Knapp, EE AF Knapp, Eric E. TI Long-term dead wood changes in a Sierra Nevada mixed conifer forest: Habitat and fire hazard implications SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Coarse woody debris; Fire hazard; Mixed conifer; Reference conditions; Snag; Tree mortality ID LODGEPOLE PINE FORESTS; CAVITY-NESTING BIRDS; RED-BACKED VOLES; OLD-GROWTH; PRESCRIBED FIRE; FUELS MANAGEMENT; NORTHERN ARIZONA; TREE MORTALITY; WESTERN OREGON; SITE SELECTION AB Dead trees play an important role in forests, with snags and coarse woody debris (CWD) used by many bird and mammal species for nesting, resting, or foraging. However, too much dead wood can also contribute to extreme fire behavior. This tension between dead wood as habitat and dead wood as fuel has raised questions about appropriate quantities in fire-dependent forested ecosystems. Three plots installed in mixed conifer forest of the central Sierra Nevada in 1929 illustrate how amounts and sizes of dead wood have changed through time as a result of logging and fire exclusion. Diameter of snags was measured and CWD was mapped in the old-growth condition, prior to logging. Snags were re-measured in 2007 or 2008, and CWD was re-mapped in 20112. Snag density increased from 15.2 ha(-1) in 1929 to 140.0 ha(-1) in 2007/2008. However, average snag size declined, with 72% and 22% of snags classified as medium or large in 1929 and 2007/2008, respectively. Mechanisms of tree mortality also appear to have changed with greater mortality in smaller size classes, possibly as a result of higher live tree density. CWD volume, mass, and cover did not differ significantly between 1929 and 2012, with increased tree mortality and lack of periodic consumption by fire apparently compensating for the loss of inputs of large wood due to past logging. However, number of logs increased from 28 ha-1 to 76 ha(-1) and average size declined substantially. Because larger-sized dead wood is preferred by many wildlife species, the current condition of more, smaller, and more decayed woody pieces may have a lower ratio of habitat value relative to potential fire hazard than it once did. Size, density, and stage of decomposition are therefore potentially better metrics for managing dead wood than mass and/or volume alone. To restore dead wood to conditions more like those found historically will require growing larger trees and reducing the inputs of dead wood from small and intermediate-sized trees. Fire, which preferentially consumes smaller and more rotten wood, would also help shift the balance to larger and less decayed pieces. Published by Elsevier B.V. C1 US Forest Serv, USDA, Pacific Southwest Res Stn, Redding, CA 96002 USA. RP Knapp, EE (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM eknapp@fs.fed.us NR 73 TC 7 Z9 7 U1 5 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2015 VL 339 BP 87 EP 95 DI 10.1016/j.foreco.2014.12.008 PG 9 WC Forestry SC Forestry GA CC6ZK UT WOS:000350517300008 ER PT J AU Zhang, X Lee, X Griffis, TJ Andrews, AE Baker, JM Erickson, MD Hu, N Xiao, W AF Zhang, Xin Lee, Xuhui Griffis, Timothy J. Andrews, Arlyn E. Baker, John M. Erickson, Matt D. Hu, Ning Xiao, Wei TI Quantifying nitrous oxide fluxes on multiple spatial scales in the Upper Midwest, USA SO INTERNATIONAL JOURNAL OF BIOMETEOROLOGY LA English DT Article DE Nitrous oxide; Corn; Soybean; Agriculture; Land surface flux ID SOIL-PLANT SYSTEMS; N2O EMISSIONS; AGRICULTURAL FIELDS; SOYBEAN RESPONSE; EDDY COVARIANCE; MAIZE; YIELD; TILLAGE; LEAVES; WHEAT AB This study seeks to quantify the roles of soybean and corn plants and the cropland ecosystem in the regional N2O budget of the Upper Midwest, USA. The N2O flux was measured at three scales (plant, the soil-plant ecosystem, and region) using newly designed steady-state flow-through plant chambers, a flux-gradient micrometeorological tower, and continuous tall-tower observatories. Results indicate that the following. (1) N2O fluxes from unfertilized soybean (0.03 +/- 0.05 nmol m(-2) s(-1)) and fertilized corn plants (-0.01 +/- 0.04 nmol m(-2) s(-1)) were about one magnitude lower than N2O emissions from the soil-plant ecosystem (0.26 nmol m(-2) s(-1) for soybean and 0.95 nmol m(-2) s(-1) for corn), confirming that cropland N2O emissions were mainly from the soil. (2) Fertilization increased the corn plant flux for a short period (about 20 days), and late-season fertilization dramatically increased the soybean plant emissions. (3) The direct N2O emission from cropland accounted for less than 20 % of the regional flux, suggesting a significant influence by other sources and indirect emissions, in the regional N2O budget. C1 [Zhang, Xin; Lee, Xuhui] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Griffis, Timothy J.; Erickson, Matt D.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Andrews, Arlyn E.] NOAA, Earth Syst Res Lab, Boulder, CO USA. [Baker, John M.] ARS, USDA, St Paul, MN USA. [Hu, Ning; Xiao, Wei] Nanjing Univ Informat Sci & Technol, Yale NUIST Ctr Atmospher Environm, Nanjing, Jiangsu, Peoples R China. RP Zhang, X (reprint author), Princeton Univ, Woodrow Wilson Sch Publ & Int Affairs, Princeton, NJ 08544 USA. EM zhangxin.yale@gmail.com RI Griffis, Timothy/A-5707-2011; Andrews, Arlyn/K-3427-2012; Xiao, Wei/G-6586-2012; Zhang, Xin/K-8264-2016 OI Xiao, Wei/0000-0002-9199-2177; Zhang, Xin/0000-0003-1619-1537 FU Ministry of Education of China (PCSIRT); Rice Family Foundation; Yale Center for Environmental Law & Policy Research Prize Fellowship; Yale Institute for Biospheric Studies; USDA [NIFA/2010-65112-20528]; NOAA's Climate Program Office; NOAA FX We would like to thank the University of Minnesota UMore Park for use of the facilities. Funding was provided by the Ministry of Education of China (grant PCSIRT), the Rice Family Foundation, the Yale Center for Environmental Law & Policy Research Prize Fellowship, the Yale Institute for Biospheric Studies, and USDA NIFA/2010-65112-20528. Measurements at the WBI tower were funded by NOAA's Climate Program Office and are part of NOAA's contributions to the North American Carbon Program. We thank Professor Charles Stanier from the University of Iowa and his students for supporting the NOAA PFP measurements at the WBI tower. NR 69 TC 13 Z9 14 U1 11 U2 47 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0020-7128 EI 1432-1254 J9 INT J BIOMETEOROL JI Int. J. Biometeorol. PD MAR PY 2015 VL 59 IS 3 BP 299 EP 310 DI 10.1007/s00484-014-0842-4 PG 12 WC Biophysics; Environmental Sciences; Meteorology & Atmospheric Sciences; Physiology SC Biophysics; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Physiology GA CB9RF UT WOS:000349969100005 PM 24879356 ER PT J AU Biasi, A Martin, F Schena, L AF Biasi, Antonio Martin, Frank Schena, Leonardo TI Identification and validation of polymorphic microsatellite loci for the analysis of Phytophthora nicotianae populations SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Phytophthora parasitica; P. nicotianae; Microsatellites; SSRs ID SUDDEN OAK DEATH; PARASITICA VAR. NICOTIANAE; INFESTANS POPULATIONS; SIMPLE SEQUENCE; FLORICULTURE CROPS; CLONAL LINEAGES; PRACTICAL GUIDE; REPEAT LOCUS; BLACK SHANK; MARKERS AB A large number of SSR loci were screened in the genomic assemblies of 14 different isolates of Phytophthora nicotianae and primers were developed for amplification of 17 markers distributed among different contigs. These loci were highly polymorphic and amplified from genetically distant isolates of the pathogen. Among these, nine were further validated using a multiplexed genotyping assay with differentially labeled primers (FAM or HEX) to allow for duplex PCR amplification. The use of reverse primers with a 5' PIG tail was important to increase the quality and reliability of the analyses. A total of 46 alleles were detected in 5 tester isolates of P. nicotianae representing the breadth of diversity in the species. Furthermore, a high incidence of heterozygosity was determined with two alleles detected in 67% of the primer/isolate combinations. Three different alleles where detected for a single locus/isolate combination, indicating variation in ploidy. These markers represent a valuable new tool for the characterization of populations of P. nicotianae. (C) 2015 Elsevier B.V. All rights reserved. C1 [Biasi, Antonio; Schena, Leonardo] Univ Mediterranea, Dipartimento Agr, I-89122 Reggio Di Calabria, Italy. [Martin, Frank] USDA ARS, Salinas, CA 93905 USA. RP Schena, L (reprint author), Univ Mediterranea, Dipartimento Agr, Localita Feo Vito, I-89122 Reggio Di Calabria, Italy. EM lschena@unirc.it OI Schena, Leonardo/0000-0002-9737-2593 FU California Department of Food and Agriculture [SB12051]; FIRB from the Italian Ministry of Education, University and Research (MIUR) [RBFR10PZ4N] FX We thank Brett Tyler, Franck Panabieres and Carsten Russ for making available the P. parasitica genome sequences at "https://olive.broadinstitute.org/projects/phytophthora_parasitica" prior to publication. Much of this work was conducted by Antonio Biasi while visiting the Martin lab and partially supported by a grant from the California Department of Food and Agriculture (2012 Specialty Crop Block Grant Program) (SB12051) and by grant FIRB 2010 (RBFR10PZ4N) from the Italian Ministry of Education, University and Research (MIUR). NR 51 TC 3 Z9 3 U1 3 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD MAR PY 2015 VL 110 BP 61 EP 67 DI 10.1016/j.mimet.2015.01.012 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA CC2RE UT WOS:000350191500011 PM 25601792 ER PT J AU Bumpus, K Tagtow, A Haven, J AF Bumpus, Kirby Tagtow, Angie Haven, Jackie TI Let's Move! Celebrates 5 Years SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Bumpus, Kirby] Off First Lady, Washington, DC USA. [Tagtow, Angie; Haven, Jackie] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. RP Bumpus, K (reprint author), Off First Lady, White House, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAR PY 2015 VL 115 IS 3 BP 338 EP 341 DI 10.1016/j.jand.2014.12.022 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CC3FM UT WOS:000350231700002 PM 25721386 ER PT J AU Liu, CK Latona, NP Taylor, M Eble, C Aldema-Ramos, ML AF Liu, Cheng-Kung Latona, Nicholas P. Taylor, Maryann Eble, Christopher Aldema-Ramos, Mila L. TI CHARACTERIZATION OF MECHANICAL PROPERTIES OF LEATHER WITH AIRBORNE ULTRASONICS SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID FIBER ORIENTATION; HIDES AB A nondestructive method to accurately evaluate the quality of hides and leather is urgently needed by leather and hide industries. Effects have been made to develop airborne ultrasonic (AU) testing method using non-contact transducers to evaluate the quality of hides and leather. We previously reported the research results demonstrating the AU technology for revealing defects in hides and leather that were difficult to be found during visual inspection. Recently new research was carried out to develop AU methods to nondestructively characterize the mechanical properties of leather. Observations showed a strong correlation between the mechanical properties of leather and the corresponding AU parameters based on the distribution of the transmission time (time of flight) through leather. We also used this nondestructive method to characterize the grain break of leather. Results showed the difference in grain break could be determined from the AU parameters collected from moving the AU sensors over a leather sample. Observations showed the poorer the grain break, the higher the time of flight distribution. In short, this study demonstrated that the tensile strength, stiffness, toughness and grain break could be nondestructively determined by AU. C1 [Liu, Cheng-Kung; Latona, Nicholas P.; Taylor, Maryann; Eble, Christopher; Aldema-Ramos, Mila L.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Liu, CK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM ChengKung.Liu@ars.usda.gov NR 21 TC 1 Z9 1 U1 1 U2 3 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD MAR PY 2015 VL 110 IS 3 BP 88 EP 93 PG 6 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA CC7AP UT WOS:000350520400004 ER PT J AU Song, CX van der Voort, M van de Mortel, J Hassan, KA Elbourne, LDH Paulsen, IT Loper, JE Raaijmakers, JM AF Song, Chunxu van der Voort, Menno van de Mortel, Judith Hassan, Karl A. Elbourne, Liam D. H. Paulsen, Ian T. Loper, Joyce E. Raaijmakers, Jos M. TI The Rsm regulon of plant growth-promoting Pseudomonas fluorescens SS101: role of small RNAs in regulation of lipopeptide biosynthesis SO MICROBIAL BIOTECHNOLOGY LA English DT Article ID SIGNAL-TRANSDUCTION PATHWAY; ESCHERICHIA-COLI; PHYTOPHTHORA-INFESTANS; BACTERIAL GENOMES; SOLUBLE-RNAS; FUMARASE C; AERUGINOSA; IDENTIFICATION; CHA0; RECOGNITION AB The rhizobacterium Pseudomonas fluorescensSS101 inhibits growth of oomycete and fungal pathogens, and induces resistance in plants against pathogens and insects. To unravel regulatory pathways of secondary metabolite production in SS101, we conducted a genome-wide search for sRNAs and performed transcriptomic analyses to identify genes associated with the Rsm (repressor of secondary metabolites) regulon. In silico analysis led to the identification of 16 putative sRNAs in the SS101 genome. In frame deletion of the sRNAs rsmY and rsmZ showed that the Rsm system regulates the biosynthesis of the lipopeptide massetolide A and involves the two repressor proteins RsmA and RsmE, with the LuxR-type transcriptional regulator MassAR as their most likely target. Transcriptome analyses of the rsmYZ mutant further revealed that genes associated with iron acquisition, motility and chemotaxis were significantly upregulated, whereas genes of the type VI secretion system were downregulated. Comparative transcriptomic analyses showed that most, but not all, of the genes controlled by RsmY/RsmZ are also controlled by the GacS/GacA two-component system. We conclude that the Rsm regulon of P.fluorescensSS101 plays a critical role in the regulation of lipopeptide biosynthesis and controls the expression of other genes involved in motility, competition and survival in the plant rhizosphere. C1 [Song, Chunxu; van der Voort, Menno; van de Mortel, Judith; Raaijmakers, Jos M.] Wageningen Univ, Lab Phytopathol, NL-6708 PD Wageningen, Netherlands. [Hassan, Karl A.; Elbourne, Liam D. H.; Paulsen, Ian T.] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia. [Loper, Joyce E.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Song, Chunxu; Raaijmakers, Jos M.] Netherlands Inst Ecol, Dept Microbial Ecol, NL-6708 PB Wageningen, Netherlands. RP Raaijmakers, JM (reprint author), Wageningen Univ, Lab Phytopathol, NL-6708 PD Wageningen, Netherlands. EM j.raaijmakers@nioo.knaw.nl RI Elbourne, Liam/G-6150-2011; Hassan, Karl/B-2802-2013; Raaijmakers, Jos/D-1574-2014; Paulsen, Ian/K-3832-2012; OI Elbourne, Liam/0000-0002-9784-0959; Raaijmakers, Jos/0000-0003-1608-6614; KNAW, NIOO-KNAW/0000-0002-3835-159X; Paulsen, Ian/0000-0001-9015-9418; Hassan, Karl/0000-0003-2031-9679; Loper, Joyce/0000-0003-3501-5969 FU Dutch Graduate School of Experimental Plant Sciences (EPS) FX We are very grateful to the Dutch Graduate School of Experimental Plant Sciences (EPS) for financing this project. NR 65 TC 3 Z9 3 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1751-7907 EI 1751-7915 J9 MICROB BIOTECHNOL JI Microb. Biotechnol. PD MAR PY 2015 VL 8 IS 2 SI SI BP 296 EP 310 DI 10.1111/1751-7915.12190 PG 15 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CC4TA UT WOS:000350345300010 PM 25488342 ER PT J AU Butte, NF Brandt, ML Wong, WW Liu, Y Mehta, NR Wilson, TA Adolph, AL Puyau, MR Vohra, FA Shypailo, RJ Zakeri, IF AF Butte, Nancy F. Brandt, Mary L. Wong, William W. Liu, Yan Mehta, Nitesh R. Wilson, Theresa A. Adolph, Anne L. Puyau, Maurice R. Vohra, Firoz A. Shypailo, Roman J. Zakeri, Issa F. TI Energetic Adaptations Persist After Bariatric Surgery in Severely Obese Adolescents SO OBESITY LA English DT Article ID Y GASTRIC BYPASS; SURGICAL WEIGHT-LOSS; FAT-FREE MASS; BODY-WEIGHT; ADAPTIVE THERMOGENESIS; METABOLIC ADAPTATION; SKELETAL-MUSCLE; PEPTIDE YY; EXPENDITURE; HOMEOSTASIS AB ObjectiveEnergetic adaptations induced by bariatric surgery have not been studied in adolescents or for extended periods postsurgery. Energetic, metabolic, and neuroendocrine responses to Roux-en-Y gastric bypass (RYGB) surgery were investigated in extremely obese adolescents. MethodsAt baseline and at 1.5, 6, and 12 months post-baseline, 24-h room calorimetry, body composition, and fasting blood biochemistries were measured in 11 obese adolescents relative to five matched controls. ResultsIn the RYGB group, mean weight loss was 44 19 kg at 12 months. Total energy expenditure (TEE), activity EE, basal metabolic rate (BMR), sleep EE, and walking EE significantly declined by 1.5 months (P = 0.001) and remained suppressed at 6 and 12 months. Adjusted for age, sex, fat-free mass, and fat mass, EE was still lower than baseline (P = 0.001). Decreases in serum insulin, leptin, and triiodothyronine (T3), gut hormones, and urinary norepinephrine (NE) paralleled the decline in EE. Adjusted changes in TEE, BMR, and/or sleep EE were associated with decreases in insulin, homeostatic model assessment, leptin, thyroid stimulating hormone, total T3, peptide YY3-36, glucagon-like peptide-2, and urinary NE and epinephrine (P = 0.001-0.05). ConclusionsEnergetic adaptations in response to RYGB-induced weight loss are associated with changes in insulin, adipokines, thyroid hormones, gut hormones, and sympathetic nervous system activity and persists 12 months postsurgery. C1 [Butte, Nancy F.; Wong, William W.; Liu, Yan; Mehta, Nitesh R.; Wilson, Theresa A.; Adolph, Anne L.; Puyau, Maurice R.; Vohra, Firoz A.; Shypailo, Roman J.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Brandt, Mary L.] Baylor Coll Med, Dept Surg, Houston, TX 77030 USA. [Zakeri, Issa F.] Drexel Univ, Dept Epidemiol & Biostat, Philadelphia, PA 19104 USA. RP Butte, NF (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM nbutte@bcm.edu OI Wong, William/0000-0002-7147-5850 FU USDA/ARS [58-6250-0-008]; National Institute of Diabetes and Digestive and Kidney Diseases [U01DK072493/UM1 DK072493]; University of Cincinnati [UM1DK095710] FX This work is an ancillary study to Teen-LABS and was funded by USDA/ARS (Cooperative Agreement No. 58-6250-0-008) and the National Institute of Diabetes and Digestive and Kidney Diseases with a grant to the Cincinnati Children's Hospital Medical Center (U01DK072493/UM1 DK072493; PI: Thomas Inge, MD, PhD) and the University of Cincinnati (UM1DK095710; PI: C. Ralph Buncher, ScD, and Todd Jenkins, PhD, MPH). The authors thank the significant contributions made by Teen-LABS Consortium as well as their parent study LABS Consortium (U01DK066557). NR 39 TC 10 Z9 10 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD MAR PY 2015 VL 23 IS 3 BP 591 EP 601 DI 10.1002/oby.20994 PG 11 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA CC3LG UT WOS:000350249700014 PM 25707380 ER PT J AU Vaghefi, SA Mousavi, SJ Abbaspour, KC Srinivasan, R Arnold, JR AF Vaghefi, S. Ashraf Mousavi, S. J. Abbaspour, K. C. Srinivasan, R. Arnold, J. R. TI Integration of hydrologic and water allocation models in basin-scale water resources management considering crop pattern and climate change: Karkheh River Basin in Iran SO REGIONAL ENVIRONMENTAL CHANGE LA English DT Article DE Hydrologic modeling; Climate change adaptation; SWAT; MODSIM ID IMPACTS; STREAMFLOW; SYSTEM; FOOD AB The paradigm of integrated water resources management requires coupled analysis of hydrology and water resources in a river basin. Population growth and uncertainties due to climate change make historic data not a reliable source of information for future planning of water resources, hence necessitating climate and landuse change impact studies. This work presents an integrated modeling approach by linking Soil and Water Assessment Tool (SWAT) and MODSIM. While SWAT produces hydrologic and water resources information, MODSIM provides a decision support system for water allocation. We used the coupled SWAT-MODSIM to analyze the effects of climate and cropping pattern changes on agricultural and hydroenergy production in the Karkheh River Basin, a semiarid region in south-west of Iran. Cropping patterns were considered by limiting the cereal production to 50 % (S1, near to historic), 17 % (S2), and 83 % (S3) of total agricultural areas. The future climate was provided by the Canadian Global Coupled Model (CGCM 3.1 version T63) for A1B, A2, and B1 scenarios. The results showed that based on future climate changes and landuse scenarios, wheat production had a large variation in five economically important agricultural regions ranging from 33,000 ton year(-1) (S2-A1B) to 74,000 ton year(-1) (S3-A2). Similarly, energy production, while increasing from 614 to 1,100 GWH in A2, decreased from 614 to 464 GWH in B1 climate scenario. Our analyses indicate that cropping pattern change can be used as an effective tool to adapt to the negative impacts of climate change. C1 [Vaghefi, S. Ashraf; Mousavi, S. J.] Amirkabir Univ Technol, Dept Civil & Environm Engn, Tehran Polytech, Tehran, Iran. [Abbaspour, K. C.] Swiss Fed Inst Aquat Sci & Technol, Eawag, CH-8600 Dubendorf, Switzerland. [Srinivasan, R.] Texas A&M Univ, Spatial Sci Lab, Texas Agr Expt Stn, College Stn, TX 77845 USA. [Arnold, J. R.] ARS, Grassland Soil & Water Res Lab, USDA, Temple, TX 76502 USA. RP Mousavi, SJ (reprint author), Amirkabir Univ Technol, Dept Civil & Environm Engn, Tehran Polytech, Hafez St 424, Tehran, Iran. EM jmosavi@aut.ac.ir RI Strauss, Peter/I-2983-2015; Srinivasan, R/D-3937-2009; Ashraf Vaghefi, Seyed Saeid/N-7632-2016 OI Strauss, Peter/0000-0002-8693-9304; Ashraf Vaghefi, Seyed Saeid/0000-0002-3061-6493 NR 28 TC 3 Z9 3 U1 4 U2 40 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-3798 EI 1436-378X J9 REG ENVIRON CHANGE JI Reg. Envir. Chang. PD MAR PY 2015 VL 15 IS 3 BP 475 EP 484 DI 10.1007/s10113-013-0573-9 PG 10 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA CC4GO UT WOS:000350310400005 ER PT J AU Yu, GT Zhang, QJ Friesen, TL Rouse, MN Jin, Y Zhong, SB Rasmussen, JB Lagudah, ES Xu, SS AF Yu, Guotai Zhang, Qijun Friesen, Timothy L. Rouse, Matthew N. Jin, Yue Zhong, Shaobin Rasmussen, Jack B. Lagudah, Evans S. Xu, Steven S. TI Identification and mapping of Sr46 from Aegilops tauschii accession CIae 25 conferring resistance to race TTKSK (Ug99) of wheat stem rust pathogen SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; HESSIAN FLY RESISTANCE; ADULT-PLANT RESISTANCE; AESTIVUM L.; MICROSATELLITE MARKERS; SPRING WHEAT; BREAD WHEAT; GENE SR36; LINES; INTROGRESSION AB Mapping studies confirm that resistance to Ug99 race of stem rust pathogen in Aegilops tauschii accession Clae 25 is conditioned by Sr46 and markers linked to the gene were developed for marker-assisted selection. The race TTKSK (Ug99) of Puccinia graminis f. sp. tritici, the causal pathogen for wheat stem rust, is considered as a major threat to global wheat production. To address this threat, researchers across the world have been devoted to identifying TTKSK-resistant genes. Here, we report the identification and mapping of a stem rust resistance gene in Aegilops tauschii accession CIae 25 that confers resistance to TTKSK and the development of molecular markers for the gene. An F-2 population of 710 plants from an Ae. tauschii cross CIae 25 x AL8/78 were first evaluated against race TPMKC. A set of 14 resistant and 116 susceptible F-2:3 families from the F-2 plants were then evaluated for their reactions to TTKSK. Based on the tests, 179 homozygous susceptible F-2 plants were selected as the mapping population to identify the simple sequence repeat (SSR) and sequence tagged site (STS) markers linked to the gene by bulk segregant analysis. A dominant stem rust resistance gene was identified and mapped with 16 SSR and five new STS markers to the deletion bin 2DS5-0.47-1.00 of chromosome arm 2DS in which Sr46 was located. Molecular marker and stem rust tests on CIae 25 and two Ae. tauschii accessions carrying Sr46 confirmed that the gene in CIae 25 is Sr46. This study also demonstrated that Sr46 is temperature-sensitive being less effective at low temperatures. The marker validation indicated that two closely linked markers Xgwm210 and Xwmc111 can be used for marker-assisted selection of Sr46 in wheat breeding programs. C1 [Yu, Guotai; Zhong, Shaobin; Rasmussen, Jack B.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Zhang, Qijun] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Friesen, Timothy L.; Xu, Steven S.] ARS, USDA, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Lagudah, Evans S.] CSIRO Agr Flagship, Canberra, ACT 2601, Australia. RP Xu, SS (reprint author), ARS, USDA, Cereal Crops Res Unit, No Crop Sci Lab, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM steven.xu@ars.usda.gov RI Rouse, Matthew/G-5474-2011 FU Bill & Melinda Gates Foundation; USDA-ARS CRIS Project [5442-22000-037-00D] FX We thank Drs. Chao-Chien Jan and Lili Qi for critically reviewing the manuscript. The authors also thank Danielle Holmes and Daryl Klindworth for technical support. This research was supported in part by funds to S. S. X. provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project and the USDA-ARS CRIS Project No. 5442-22000-037-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 64 TC 4 Z9 4 U1 0 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2015 VL 128 IS 3 BP 431 EP 443 DI 10.1007/s00122-014-2442-4 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CC0QT UT WOS:000350041500006 PM 25523501 ER PT J AU Hao, YF Parks, R Cowger, C Chen, ZB Wang, YY Bland, D Murphy, JP Guedira, M Brown-Guedira, G Johnson, J AF Hao, Yuanfeng Parks, Ryan Cowger, Christina Chen, Zhenbang Wang, Yingying Bland, Dan Murphy, J. Paul Guedira, Mohammed Brown-Guedira, Gina Johnson, Jerry TI Molecular characterization of a new powdery mildew resistance gene Pm54 in soft red winter wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID ADULT-PLANT RESISTANCE; F-SP TRITICI; QUANTITATIVE TRAIT LOCI; COMMON WHEAT; CHROMOSOMAL LOCATION; NORTH-CAROLINA; MAJOR QTL; EM THELL.; REGISTRATION; MARKERS AB A new powdery mildew resistance gene Pm54 was identified on chromosome 6BL in soft red winter wheat. Powdery mildew is causing increasing damage to wheat production in the southeastern USA. To combat the disease, a continuing need exists to discover new genes for powdery mildew resistance and to incorporate those genes into breeding programs. Pioneer(A (R)) variety 26R61 (shortened as 26R61) and AGS 2000 have been used as checks in the Uniform Southern Soft Red Winter Wheat Nursery for a decade, and both have provided good resistance across regions during that time. In the present study, a genetic analysis of mildew resistance was conducted on a RIL population developed from a cross of 26R61 and AGS 2000. Phenotypic evaluation was conducted in the field at Plains, GA, and Raleigh, NC, in 2012 and 2013, a total of four environments. Three quantitative trait loci (QTL) with major effect were consistently detected on wheat chromosomes 2BL, 4A and 6BL. The 2BL QTL contributed by 26R61 was different from Pm6, a widely used gene in the southeastern USA. The other two QTL were identified from AGS 2000. The 6BL QTL was subsequently characterized as a simple Mendelian factor when the population was inoculated with a single Blumeria graminis f. sp. tritici (Bgt) isolate in controlled environments. Since there is no known powdery mildew resistance gene (Pm) on this particular location of common wheat, the gene was designated Pm54. The closely linked marker Xbarc134 was highly polymorphic in a set of mildew differentials, indicating that the marker should be useful for pyramiding Pm54 with other Pm genes by marker-assisted selection. C1 [Hao, Yuanfeng; Chen, Zhenbang; Wang, Yingying; Bland, Dan; Johnson, Jerry] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. [Parks, Ryan; Cowger, Christina] N Carolina State Univ, USDA ARS Plant Sci Res, Dept Plant Pathol, Raleigh, NC 27695 USA. [Murphy, J. Paul; Guedira, Mohammed] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Brown-Guedira, Gina] N Carolina State Univ, USDA ARS Plant Sci Res, Dept Crop Sci, Raleigh, NC 27695 USA. RP Johnson, J (reprint author), Univ Georgia, Dept Crop & Soil Sci, Griffin Campus, Griffin, GA 30223 USA. EM y.hao@cgiar.org; Ryan.Parks@ars.usda.gov; Christina.Cowger@ars.usda.gov; zchen@uga.edu; yywang@uga.edu; sgrain@uga.edu; paul_murphy@ncsu.edu; mguedir@ncsu.edu; Gina.Brown-Guedira@ars.usda.gov; jjohnso@uga.edu OI Guedira, Mohammed/0000-0003-3953-6112 FU National Research Initiative of USDA's Cooperative State Research, Education and Extension Service, CAP [2006-55606-16629] FX The authors acknowledge financial support by the National Research Initiative of USDA's Cooperative State Research, Education and Extension Service, CAP (Grant No. 2006-55606-16629). Critical review of the manuscript from Dr. R.A. McIntosh is highly appreciated. NR 49 TC 19 Z9 22 U1 3 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2015 VL 128 IS 3 BP 465 EP 476 DI 10.1007/s00122-014-2445-1 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CC0QT UT WOS:000350041500009 PM 25533209 ER PT J AU Qi, LL Ma, GJ Long, YM Hulke, BS Gong, L Markell, SG AF Qi, L. L. Ma, G. J. Long, Y. M. Hulke, B. S. Gong, L. Markell, S. G. TI Relocation of a rust resistance gene R (2) and its marker-assisted gene pyramiding in confection sunflower (Helianthus annuus L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID PUCCINIA-HELIANTHI; DOWNY MILDEW; GERMPLASM LINES; POSSESSING GENES; PARENTAL LINES; MOSAIC-VIRUS; NORTH-DAKOTA; LEAF RUST; REGISTRATION; SELECTION AB The rust resistance gene R (2) was reassigned to linkage group 14 of the sunflower genome. DNA markers linked to R (2) were identified and used for marker-assisted gene pyramiding in a confection type genetic background. Due to the frequent evolution of new pathogen races, sunflower rust is a recurring threat to sunflower production worldwide. The inbred line Morden Cross 29 (MC29) carries the rust resistance gene, R (2) , conferring resistance to numerous races of rust fungus in the US, Canada, and Australia, and can be used as a broad-spectrum resistance resource. Based on phenotypic assessments and SSR marker analyses on the 117 F-2 individuals derived from a cross of HA 89 with MC29 (USDA), R (2) was mapped to linkage group (LG) 14 of the sunflower, and not to the previously reported location on LG9. The closest SSR marker HT567 was located at 4.3 cM distal to R (2) . Furthermore, 36 selected SNP markers from LG14 were used to saturate the R (2) region. Two SNP markers, NSA_002316 and SFW01272, flanked R (2) at a genetic distance of 2.8 and 1.8 cM, respectively. Of the three closely linked markers, SFW00211 amplified an allele specific for the presence of R (2) in a marker validation set of 46 breeding lines, and SFW01272 was also shown to be diagnostic for R (2) . These newly developed markers, together with the previously identified markers linked to the gene R (13a) , were used to screen 524 F-2 individuals from a cross of a confection R (2) line and HA-R6 carrying R (13a) . Eleven homozygous double-resistant F-2 plants with the gene combination of R (2) and R (13a) were obtained. This double-resistant line will be extremely useful in confection sunflower, where few rust R genes are available, risking evolution of new virulence phenotypes and further disease epidemics. C1 [Qi, L. L.; Hulke, B. S.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. [Ma, G. J.; Gong, L.; Markell, S. G.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Long, Y. M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Qi, LL (reprint author), ARS, USDA, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU USDA-AMS [12-25-B-1480]; USDA-ARS CRIS [5442-21000-039-00D] FX We thank Drs. Gerald Seiler and Gongjun Shi for critical review of the manuscript, and Angelia Hogness for technical assistance. This project was supported by the USDA-AMS Cooperative Agreement 12-25-B-1480 and the USDA-ARS CRIS Project No. 5442-21000-039-00D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 64 TC 6 Z9 6 U1 1 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2015 VL 128 IS 3 BP 477 EP 488 DI 10.1007/s00122-014-2446-0 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CC0QT UT WOS:000350041500010 PM 25575836 ER PT J AU Shen, XK Ma, LX Zhong, SF Liu, N Zhang, M Chen, WQ Zhou, YL Li, HJ Chang, ZJ Li, X Bai, GH Zhang, HY Tan, FQ Ren, ZL Luo, PG AF Shen, X. K. Ma, L. X. Zhong, S. F. Liu, N. Zhang, M. Chen, W. Q. Zhou, Y. L. Li, H. J. Chang, Z. J. Li, X. Bai, G. H. Zhang, H. Y. Tan, F. Q. Ren, Z. L. Luo, P. G. TI Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID STRIPE RUST; COMMON WHEAT; CONFERRING RESISTANCE; MOLECULAR MARKERS; TRITICUM-AESTIVUM; DRAFT GENOME; BREAD WHEAT; WILD EMMER; MAP; DISEASES AB Powdery resistance putatively derived from Thinopyrum intermedium in the wheat line L962 is controlled by a single dominant gene designated PmL962 and mapped to chromosome arm 2BS. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease affecting the production of wheat (Triticum aestivum). Powdery mildew resistance was putatively transferred from Thinopyrum intermedium to the common wheat line L962, which conferred resistance to multiple Chinese Bgt isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F-1, F-2, and F-2:3 populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F-2:3 lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated PmL962. It is flanked by Xwmc314 and BE443737at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in Brachypodium distachyon and a 223.5 kb genomic region on rice (Oryza sativa) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding. C1 [Shen, X. K.; Chen, W. Q.; Zhou, Y. L.; Luo, P. G.] CAAS, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China. [Shen, X. K.; Ma, L. X.; Zhong, S. F.; Liu, N.; Zhang, M.; Li, X.; Zhang, H. Y.; Tan, F. Q.; Ren, Z. L.; Luo, P. G.] Sichuan Agr Univ, Prov Key Lab Plant Breeding & Genet, Chengdu 611130, Sichuan, Peoples R China. [Li, H. J.] Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China. [Chang, Z. J.] Shanxi Acad Agr Sci, Inst Crop Genet, Taiyuan 030031, Shanxi, Peoples R China. [Bai, G. H.; Luo, P. G.] ARS, Hard Winter Wheat Genet Res Unit, USDA, Manhattan, KS 66506 USA. RP Luo, PG (reprint author), CAAS, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China. EM lpglab@sicau.edu.cn FU National Natural Science Foundation of China [31271721]; Provincial Science and Technology Foundation for Young Scientists of Sichuan China [2010JQ0042]; State Key Laboratory for Biology of Plant Disease and Insect Pests, China [SKLOF201410]; Specific Foundation of Agronomy [nyhyzx3-15, 201303016]; Key Project of the Education Ministry of China [2012146]; Ministry of Science and Technology of China [2011CB100403, 2013CB127701] FX Financial support was provided by the National Natural Science Foundation of China (31271721), the Provincial Science and Technology Foundation for Young Scientists of Sichuan China (2010JQ0042), the State Key Laboratory for Biology of Plant Disease and Insect Pests, China (SKLOF201410), the Specific Foundation of Agronomy (No. nyhyzx3-15, 201303016), the Key Project of the Education Ministry of China (2012146), and the Ministry of Science and Technology of China (2011CB100403 and 2013CB127701). We are grateful to Dr. R. A. McIntosh (University of Sydney, Australia) and Dr. X. C. Xia [Institute of Crop Science at the National Wheat Improvement Centre of The National Key Facility for Crop Gene Resources and Genetic Improvement of the Chinese Academy of Agricultural Sciences (CAAS) in Beijing, China] for critically reviewing drafts of this paper. We are also grateful to Prof Z. Y. Liu, College of Agriculture and Biotechnology, China Agricultural University, Beijing, for providing many useful suggestions and discussing the manuscript. NR 49 TC 6 Z9 6 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2015 VL 128 IS 3 BP 517 EP 528 DI 10.1007/s00122-014-2449-x PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CC0QT UT WOS:000350041500013 PM 25556931 ER PT J AU Palmieri, C Anthenill, L Shivaprasad, HL AF Palmieri, C. Anthenill, L. Shivaprasad, H. L. TI Cutaneous Mucinosis in a Strain of Brown-Egg Laying Chickens SO VETERINARY PATHOLOGY LA English DT Article DE mucinosis; skin; histology; brown egg-laying chickens ID SYSTEMIC-LUPUS-ERYTHEMATOSUS; GLYCOSAMINOGLYCAN SYNTHESIS; DERMAL FIBROBLASTS; HYALURONAN; DOGS; SKIN; STIMULATION; CULTURES; BETA AB Cutaneous mucinosis is a cutaneous disorder described in humans, dogs, and rarely cats but never reported in birds. Twenty-six brown egg-laying chickens between ages 43 and 46 weeks had a history of feather loss, scaly, dry skin, weight loss, and decreased egg production. Microscopic findings in the skin included fragmentation of collagen bundles and interstitial, periadnexal, and perivascular dermal accumulation of wispy, mildly basophilic material that was also occasionally observed within the follicular epithelium. A moderate lymphoplasmacytic and heterophilic perivascular dermatitis was also observed. The wispy to granular material was diffusely Alcian blue positive and periodic acid-Schiff negative (consistent with mucin), suggesting a diagnosis of primary or secondary cutaneous mucinosis. The cause of this condition could not be determined. C1 [Palmieri, C.] Univ Queensland, Sch Vet Sci, Brisbane, Qld 4072, Australia. [Anthenill, L.] US Food Safety & Inspect Serv, USDA, Off Field Operat, Washington, DC 20250 USA. [Anthenill, L.] Calif Anim Hlth & Food Safety Lab Syst, San Bernardino, CA USA. [Shivaprasad, H. L.] Calif Anim Hlth & Food Safety Lab Syst, Tulare, CA USA. [Shivaprasad, H. L.] Univ Calif Davis, Sch Vet Med, Tulare, CA 93274 USA. RP Shivaprasad, HL (reprint author), Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, 18830 Rd 112, Tulare, CA 93274 USA. EM hlshivaprasad@ucdavis.edu OI Palmieri, Chiara/0000-0002-5791-6066 NR 36 TC 0 Z9 0 U1 1 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 EI 1544-2217 J9 VET PATHOL JI Vet. Pathol. PD MAR PY 2015 VL 52 IS 2 BP 351 EP 355 DI 10.1177/0300985814535642 PG 5 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA CC7NX UT WOS:000350556100014 PM 24942711 ER PT J AU Wada, S Maki, S Niedz, RP Reed, BM AF Wada, Sugae Maki, Shinya Niedz, Randall P. Reed, Barbara M. TI Screening genetically diverse pear species for in vitro CaCl2, MgSO4 and KH2PO4 requirements SO ACTA PHYSIOLOGIAE PLANTARUM LA English DT Article DE Growth medium; Mesos components; Micropropagation; Mineral nutrition; Pyrus ID TOBACCO TISSUE CULTURES; GROWTH; MEDIA; L. AB Conservation of important plant germplasm is often difficult due to the specific growth requirements of genetically diverse species including in vitro culture collections. Recently the mesos components (CaCl2, MgSO4, KH2PO4) of Murashige and Skoog medium were identified as one of the most influential groups of nutrients for five pear genotypes. To determine if this requirement also applied to a larger germplasm collection, 18 genotypes in six species were screened. Shoot quality, shoot length, leaf spots and leaf color were the most affected responses. Seven of nine Pyrus communis cultivars had improved shoot quality, five had significantly longer shoots, better leaf color and fewer leaf spots while two had more shoots. Two of the four Pyrus pyrifolia cultivars had improved shoot quality while three had better leaf color and fewer leaf spots. Pyrus calleryana 'Capital', Pyrus cordata and Pyrus ussuriensis 'Harbin' had longer shoots while Pyrus koehnei had less callus. P. ussuriensis 'Hang Pa Li' was the only genotype where shoot quality declined at high mesos concentrations. Quantitative ion analysis detected substantially higher concentrations of Ca, Mg and K, but significantly less Fe, in the shoots cultured on increased mesos compared to controls. This study confirms that increased mesos improved growth of P. communis and P. pyrifolia cultivars, but produced fewer significantly improved responses for four other species. C1 [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Maki, Shinya] Niihama Natl Coll Technol, Dept Appl Chem & Biotechnol, Niihama, Ehime, Japan. [Niedz, Randall P.] USDA, US Hort Res Lab, Agr Res Serv, Ft Pierce, FL 34945 USA. [Reed, Barbara M.] USDA, Agr Res Serv, Corvallis, OR 97333 USA. RP Wada, S (reprint author), Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. EM Sugae.Wada@oregonstate.edu; maki@chem.niihama-nct.ac.jp; Randall.Niedz@ars.usda.gov; Barbara.Reed@ars.usda.gov FU Oregon Association of Nurseries; Oregon Department of Agriculture; USDA-ARS CRIS [5358-21000-0-38-00D] FX We thank NCGR lab personnel for assistance with collection of the data. This project was funded by a grant from the Oregon Association of Nurseries and the Oregon Department of Agriculture and by USDA-ARS CRIS project 5358-21000-0-38-00D. NR 27 TC 1 Z9 1 U1 3 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0137-5881 EI 1861-1664 J9 ACTA PHYSIOL PLANT JI Acta Physiol. Plant. PD MAR PY 2015 VL 37 IS 3 DI 10.1007/s11738-014-1754-y PG 10 WC Plant Sciences SC Plant Sciences GA CC0TU UT WOS:000350050500021 ER PT J AU Casler, MD Vermerris, W Dixon, RA AF Casler, Michael D. Vermerris, Wilfred Dixon, Richard A. TI Replication Concepts for Bioenergy Research Experiments SO BIOENERGY RESEARCH LA English DT Review DE ANOVA; Experimental design; Feedstock; Generalized linear mixed models; Linear mixed models; Power; Replication; Randomization; Repeated measures ID STATISTICAL-ANALYSIS; FIELD EXPERIMENTS; MICROARRAY DATA; DESIGN; VARIABILITY; EFFICIENCY; ACCURACY; NUMBERS; YIELD; PLOTS AB While there are some large and fundamental differences among disciplines related to the conversion of biomass to bioenergy, all scientific endeavors involve the use of biological feedstocks. As such, nearly every scientific experiment conducted in this area, regardless of the specific discipline, is subject to random variation, some of which is unpredictable and unidentifiable (i.e., pure random variation such as variation among plots in an experiment, individuals within a plot, or laboratory samples within an experimental unit) while some is predictable and identifiable (repeatable variation, such as spatial or temporal patterns within an experimental field, a glasshouse or growth chamber, or among laboratory containers). Identifying the scale and sources of this variation relative to the specific hypotheses of interest is a critical component of designing good experiments that generate meaningful and believable hypothesis tests and inference statements. Many bioenergy feedstock experiments are replicated at an incorrect scale, typically by sampling feedstocks to estimate laboratory error or by completely ignoring the errors associated with growing feedstocks in an agricultural area at a field or farmland (micro- or macro-region) scale. As such, actual random errors inherent in experimental materials are frequently underestimated, with unrealistically low standard errors of statistical parameters (e.g., means), leading to improper inferences. The examples and guidelines set forth in this paper and many of the references cited are intended to form the general policy and guidelines for replication of bioenergy feedstock experiments to be published in BioEnergy Research. C1 [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Vermerris, Wilfred] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32610 USA. [Vermerris, Wilfred] Univ Florida, UF Genet Inst, Gainesville, FL 32610 USA. [Dixon, Richard A.] Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA. [Dixon, Richard A.] Oak Ridge Natl Lab, BioEnergy Sci Ctr BESC, US DOE, Oak Ridge, TN 37831 USA. RP Casler, MD (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM michael.casler@ars.usda.gov FU USDA-ARS; USDA-NIFA Biomass Research and Development [2011-10006-30358]; U.S. DOE EERE BTO/U.S. DOE International Affairs [DE-PI0000031]; Southeastern SunGrant Center; USDA-NIFA [2010-38502-21854]; US Department of Energy's Bioenergy Sciences Center - Office of Biological and Environmental Research in the DOE Office of Science [BER DE-AC05-00OR22725] FX We thank the following members of the BioEnergy Research editorial board for making the time to review various versions of this manuscript and provide suggestions and constructive criticism on the manuscript: Angela Karp, Rothamstead Research Harpendon, Hertfordshire, UK; Antje Hermann, Christian-Albrechts- Universitat zu Kiel, Germany; Ronald Zalesny, USDA Forest Service, Rhinelander, WI, USA; JY Zhu, USDA Forest Service, Madison, WI, USA; and Edzard van Santen, Auburn University, Auburn, AL, USA. We also thank two anonymous peer reviewers for their constructive comments that were helpful in improving the manuscript. Funding for MDC was provided by congressionally allocated funds through USDA-ARS. Funding for WV was provided by USDA-NIFA Biomass Research and Development grant No. 2011-10006-30358, U.S. DOE EERE BTO/U.S. DOE International Affairs award No. DE-PI0000031, and Southeastern SunGrant Center and USDA-NIFA award No. 2010-38502-21854. Funding for RAD was provided from the US Department of Energy's Bioenergy Sciences Center, supported by the Office of Biological and Environmental Research in the DOE Office of Science (BER DE-AC05-00OR22725). NR 36 TC 3 Z9 3 U1 1 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2015 VL 8 IS 1 BP 1 EP 16 DI 10.1007/s12155-015-9580-7 PG 16 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CC0UA UT WOS:000350051300001 ER PT J AU Koch, KG Palmer, N Stamm, M Bradshaw, JD Blankenship, E Baird, LM Sarath, G Heng-Moss, TM AF Koch, Kyle G. Palmer, Nathan Stamm, Mitch Bradshaw, Jeff D. Blankenship, Erin Baird, Lisa M. Sarath, Gautam Heng-Moss, Tiffany M. TI Characterization of Greenbug Feeding Behavior and Aphid (Hemiptera: Aphididae) Host Preference in Relation to Resistant and Susceptible Tetraploid Switchgrass Populations SO BIOENERGY RESEARCH LA English DT Article DE Choice studies; EPG; Feeding behavior; Greenbug; Plant resistance; Tetraploid switchgrass; Yellow sugarcane aphid ID SIEVE ELEMENT ACCEPTANCE; CALCIUM-OXALATE CRYSTALS; XYLEM INGESTION; MYZUS-PERSICAE; CEREAL APHIDS; GROWTH-STAGES; HOMOPTERA; PENETRATION; PHLOEM; ANTIXENOSIS AB Two choice studies were performed to evaluate greenbug, Schizaphis graminum (Rondani), and yellow sugarcane aphid, Sipha flava (Forbes), preference for two tetraploid switchgrass populations, Summer and Kanlow, and one experimental hybrid, K x S, derived by crossing Kanlow (male) x Summer (female) plants. Additionally, an assessment of S. graminum feeding behavior was performed on the same switchgrass populations, by using the electrical penetration graph (EPG) technique. Choice studies for S. flava indicated a lack of antixenosis, with no preference by aphids among any of the switchgrass populations at any time point. However, choice studies with S. graminum indicated a preference for the K x S plants at 24 h after aphid introduction. No obvious differences were observed for the leaf surfaces between the three populations. Feeding behavior studies for S. graminum on switchgrasses indicated no differences for the time to first probe or time to first sieve element phase among switchgrass populations. However, duration of sieve element phases for S. graminum was significantly less on Kanlow compared to K x S and Summer. S. graminum also had a significantly lower potential phloem ingestion index (PPII) and few aphids showing sustained phloem ingestion on Kanlow as compared to K x S and Summer plants. These results suggest that resistance factors (chemical or mechanical) in Kanlow are located in the phloem tissue. At the whole leaf level, some differences were observed for a subset of polar metabolites, although Kanlow plants were significantly enriched for oxalic acid. C1 [Koch, Kyle G.; Stamm, Mitch; Heng-Moss, Tiffany M.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Palmer, Nathan; Sarath, Gautam] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Bradshaw, Jeff D.] Univ Nebraska, Dept Entomol, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. [Blankenship, Erin] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. [Baird, Lisa M.] Univ San Diego, Dept Biol, San Diego, CA 92110 USA. RP Heng-Moss, TM (reprint author), Univ Nebraska, Dept Entomol, 201B Entomol Hall, Lincoln, NE 68583 USA. EM thengmoss2@unl.edu FU USDA-National Institute of Food and Agriculture [2011-67009-30096]; USDA-ARS CRIS [5440-21000-030-00D] FX We gratefully acknowledge Z. B. Mayo for reviewing the manuscript and K. P. Vogel for providing the switchgrass germ plasm for these studies. We thank Dr. Han Chen, Microscopy Core Facility, University of Nebraska at Lincoln, for the help with the SEM. This research was supported in part by the USDA-National Institute of Food and Agriculture grant number 2011-67009-30096 and by the USDA-ARS CRIS project 5440-21000-030-00D. The US Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. NR 51 TC 2 Z9 2 U1 2 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2015 VL 8 IS 1 BP 165 EP 174 DI 10.1007/s12155-014-9510-0 PG 10 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CC0UA UT WOS:000350051300015 ER PT J AU Lazarus, W Headlee, WL Zalesny, RS AF Lazarus, William Headlee, William L. Zalesny, Ronald S. TI Impacts of Supplyshed-Level Differences in Productivity and Land Costs on the Economics of Hybrid Poplar Production in Minnesota, USA SO BIOENERGY RESEARCH LA English DT Article DE Poplar; Economics; Land rent; Discount rate; Biomass; 3-PG ID BIOMASS; POSITION; SYSTEMS AB The joint effects of poplar biomass productivity and land costs on poplar production economics were compared for 12 Minnesota counties and two genetic groups, using a process-based model (3-PG) to estimate aboveground biomass productivity. The counties represent three levels of productivity which, due to spatial stratification, were analogous to three biomass supplysheds. An optimal rotation age (ORA) was calculated that minimizes the annualized, discounted per-dry megagrams biomass cost for each county, genetic group and land cover, and for two discount rates (5 and 10 %). The ORA for the lowest-cost county (Todd) with specialist genotypes and a 5 % discount rate is 14 years and the breakeven price at that age is US$71 dry Mg-1, while for the highest-cost county (McLeod), the generalist genotype and a 10 % discount rate, the ORA is 10 years and the breakeven price at that age is US$175 dry Mg-1. Planting after a previous poplar stand increased breakeven prices and increased the ORAs by 1 to 2 years relative to planting after a previous annual crop. An ANOVA analysis showed a significant genetic group effect and significant productivity class x land rent interactions. All other factors being equal, an increase in the discount rate from 5 to 10 % is expected to reduce ORAs by 2 to 3 years. High-productivity supplysheds can also be expected to have ORAs that are 2 to 3 years shorter than low-productivity ones. Land costs were not as closely correlated to productivity as we expected. C1 [Lazarus, William] Univ Minnesota, Dept Appl Econ, St Paul, MN 55108 USA. [Headlee, William L.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Zalesny, Ronald S.] US Forest Serv, No Res Stn, USDA, Rhinelander, WI USA. RP Lazarus, W (reprint author), Univ Minnesota, Dept Appl Econ, 249 Ruttan Hall,1994 Buford Ave, St Paul, MN 55108 USA. EM wlazarus@umn.edu OI Lazarus, William/0000-0003-3133-2318 FU U.S. Forest Service Northern Research Station, Institute for Applied Ecosystem Studies [RWU-NRS-13] FX This study was funded by the U.S. Forest Service Northern Research Station, Institute for Applied Ecosystem Studies (RWU-NRS-13). We thank Sue Lietz for creating Fig. 1 and our experts for providing information on poplar production practices. We are also grateful to Brad Bender and Gregg Johnson for review of earlier versions of the manuscript. NR 35 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2015 VL 8 IS 1 BP 231 EP 248 DI 10.1007/s12155-014-9520-y PG 18 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CC0UA UT WOS:000350051300020 ER PT J AU Zhang, JZ Laguna, A Clemons, C Wolcott, MP Gleisner, R Zhu, JY Zhang, X AF Zhang, Jingzhi Laguna, Andrea Clemons, Craig Wolcott, Michael P. Gleisner, Rolland Zhu, J. Y. Zhang, Xu TI Effect of Hot-Pressing Temperature on the Subsequent Enzymatic Saccharification and Fermentation Performance of SPORL Pretreated Forest Biomass SO BIOENERGY RESEARCH LA English DT Article DE Biomass commoditization; Supply chain logistics; Densification; Water retention value; Cellulase adsorption; Enzymatic hydrolysis ID LIGNOCELLULOSES; LOGISTICS; SCALE AB Methods to increase the energy density of biofuel feedstock for shipment are important towards improving supply chain efficiency in upstream processes. Towards this end, densified pretreated lignocellulosic biomass was produced using hot-pressing. The effects of fiber hornification induced by hot-pressing on enzymatic digestibilities of lodgepole pine and poplar NE222 wood chips pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) were examined. Pretreated wood chips were pressed at 25, 70, 90, 110, and 177 A degrees C. The cellulose accessibilities of the pressed and unpressed substrates were evaluated using water retention value and direct cellulase adsorption measurements. Hot-pressing below 110 A degrees C produced a degree of hornification (DH) below 0.26 and had limited effect on cellulose accessibility and enzymatic digestibility. Hot-pressing at 177 A degrees C produced a DH of 0.86 that substantially hornified the fibers and resulted near zero saccharification. The saccharification results were consistent with cellulose accessibility data. Ethanol fermentation studies at 18 % solids suggest that a pressing below 110 A degrees C is preferred to reduce its effect on biofuel yield. C1 [Zhang, Jingzhi; Zhang, Xu] Beijing Univ Chem Technol, Sch Life Sci & Technol, Beijing 100029, Peoples R China. [Zhang, Jingzhi; Laguna, Andrea; Clemons, Craig; Gleisner, Rolland; Zhu, J. Y.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. [Laguna, Andrea] Univ Wisconsin, Madison, WI USA. [Wolcott, Michael P.] Washington State Univ, Dept Civil & Environm Engn, Pullman, WA 99164 USA. RP Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, USDA, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM jzhu@fs.fed.us FU Agriculture and Food Research Initiative Competitive grant [2011-68005-30416]; USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA); Chinese Scholarship Council (CSC); USDA-NIFA FX We would like to acknowledge the financial support from the Agriculture and Food Research Initiative Competitive grant (no. 2011-68005-30416) and USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA) and the Chinese Scholarship Council (CSC). These two programs made the visiting appointment of Jinzhi Zhang at the USDA Forest Products Lab (FPL) possible. The USDA-NIFA funding also supported Andrea Laguna's summer internship at FPL through the Washington State University Summer Undergraduate Research Program. We also would like to acknowledge Phil Walsh and Fred Matt of FPL for pressing the chips and carbohydrate analysis, respectively. NR 23 TC 1 Z9 1 U1 2 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2015 VL 8 IS 1 BP 464 EP 470 DI 10.1007/s12155-014-9530-9 PG 7 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CC0UA UT WOS:000350051300040 ER PT J AU Fischer, MJ Havill, NP Brewster, CC Davis, GA Salom, SM Kok, LT AF Fischer, Melissa J. Havill, Nathan P. Brewster, Carlyle C. Davis, Gina A. Salom, Scott M. Kok, Loke T. TI Field assessment of hybridization between Laricobius nigrinus and L. rubidus, predators of Adelgidae SO BIOLOGICAL CONTROL LA English DT Article DE Hemlock woolly adelgid; Laricobius nigrinus; Laricobius rubidus; Hybridization; Biological control ID COLEOPTERA DERODONTIDAE; BIOLOGICAL-CONTROL; NORTH-AMERICA; SPECIATION; REINFORCEMENT AB Two adelgid predators, Laricobius nigrinus Fender and Laricobius rubidus LeConte, were recently discovered to produce hybrid progeny in the eastern United States. Laricobius rubidus is native to eastern North America where it feeds on pine bark adelgid (Pineus strobi Hartig) and Laricobius nigrinus is native to western North America and was introduced to the eastern United States in 2003 for biological control of hemlock woolly adelgid (Adelges tsugae Annand). Currently, Laricobius nigrinus and Laricobius rubidus form a mosaic of hybrid zones throughout the eastern United States. It is not known whether these zones will be maintained over time and whether hybridization will impact the efficacy of biological control or result in displacement of Laricobius rubidus. Sampling from 2007 to 2012 on eastern hemlock (Tsuga canadensis Carriere) showed a clear increase in Laricobius nigrinus, a decrease in Laricobius rubidus, and a steady proportion of hybrids. Sampling from 2010 to 2012 on both eastern hemlock and white pine (Pinus strobus L.) at ten sites showed that Laricobius nigrinus was more dominant on hemlock and Laricobius rubidus was more dominant on white pine, which may demonstrate habitat preference and promote segregation between the two species. Site factors were tested for a relationship with the proportion of hybrids. The number of years Laricobius nigrinus had been present at the site was the only factor that showed a relationship. The results suggest that Laricobius nigrinus may displace Laricobius rubidus on hemlock, but not on white pine at sites where they are both present. (C) 2014 Elsevier Inc. All rights reserved. C1 [Fischer, Melissa J.; Brewster, Carlyle C.; Salom, Scott M.; Kok, Loke T.] Virginia Tech, Blacksburg, VA 24061 USA. [Havill, Nathan P.] US Forest Serv, USDA, No Res Stn, Hamden, CT 06514 USA. [Davis, Gina A.] Idaho Dept Lands, Coeur Dalene, ID 83815 USA. RP Fischer, MJ (reprint author), Washington State Dept Nat Resources, 225 S Silke Rd, Colville, WA 99114 USA. EM Melissa.Fischer@dnr.wa.gov RI Kok, Loke/G-9922-2016 OI Kok, Loke/0000-0002-5734-3472 FU USDA Forest Service [07-CA-11420004-161, 11-CA-11420004-133] FX Thanks to Peter Thomasson, Chris Philips, Ian Firkin, Linda Manden, Brad Onken, and Biff Thompson for help in the field; to Carrie Jubb, Kara Tourje, Natalie Morris, Ryan Mays, Tom McAvoy and Christine Cho for their help at the Virginia Tech Insectary and Quarantine Lab, and to Joanne Klein, Brenna Traver, Stephanie Vece, Jacqueline Martin, Chandler Barton, and Grace Mulholland for their help in the molecular lab. This work was supported by USDA Forest Service Cooperative Agreements 07-CA-11420004-161 and 11-CA-11420004-133. NR 25 TC 4 Z9 4 U1 0 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2015 VL 82 BP 1 EP 6 DI 10.1016/j.biocontrol.2014.12.002 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CB9OC UT WOS:000349960100001 ER PT J AU Shapiro-Ilan, DI Cottrell, TE Mizell, RF Horton, DL Zaid, A AF Shapiro-Ilan, David I. Cottrell, Ted E. Mizell, Russell F., III Horton, Dan L. Zaid, Abdo TI Field suppression of the peachtree borer, Synanthedon exitiosa, using Steinernema carpocapsae: Effects of irrigation, a sprayable gel and application method SO BIOLOGICAL CONTROL LA English DT Article DE Application method; Entomopathogenic nematode; Formulation; Irrigation; Peachtree borer; Steinernema carpocapsae; Synanthedon exitiosa ID ENTOMOPATHOGENIC NEMATODES; BIOLOGICAL-CONTROL; APPLICATION TECHNOLOGY; APPLE ORCHARDS; CODLING MOTH; LEPIDOPTERA; RHABDITIDA; EFFICACY; SESIIDAE; TORTRICIDAE AB The peachtree borer, Synanthedon exitiosa, is a major pest of stone fruit trees in North America. In prior studies, the entomopathogenic nematode, Steinernema carpocapsae, caused substantial reductions in Synanthedon exitiosa damage when applied by watering can to peach trees that were irrigated regularly. Here we report two additional studies that assessed Steinernema carpocapsae efficacy in suppressing Synanthedon exitiosa damage in peach orchards; one study focused on irrigation requirements and the other on application method. In the first experiment we compared Steinernema carpocapsae applied with and without irrigation, and application of a sprayable gel, Barricade(R), as a potential replacement for irrigation. In the second experiment, we compared application methods that growers might use including a boom sprayer, handgun, trunk sprayer and watering can (used as a positive control). In both experiments chlorpyrifos was also included as a positive control, and in the application methods experiment an untreated (negative) control was also included. All treatments were applied in the fall of 2012 and 2013 and Synanthedon exitiosa infestation was assessed following the spring of 2013 and 2014, respectively. In the first experiment, nematodes applied without irrigation did not prevent high levels of infestation levels (75% of trees were infested) whereas nematodes applied with the sprayable gel suppressed damage at the same level as chlorpyrifos (<20% infestation). Thus, our results indicate that the sprayable gel applied to soil around the tree base can enhance entomopathogenic nematode efficacy, and the gel may be used as a substitute for irrigation when applying Steinernema carpocapsae for Synanthedon exitiosa control; this finding may be applicable to similar pests in various cropping systems. This is the first report of direct application of the sprayable gel to soil (previous reports concerned aboveground applications). Also in the first experiment, intermediate levels of damage (31-38% infestation) were observed in plots that received nematodes with irrigation. We suspect that a higher rate of irrigation would have improved efficacy. In the second experiment, the boom sprayer, trunk sprayer and watering can methods of nematode application resulted in Synanthedon exitiosa infestations that were similar to the chemical insecticide standard treatment (chlorpyrifos) and below levels in the non-treated control, whereas the handgun treatment was not different from the untreated control or chemical standard. Published by Elsevier Inc. C1 [Shapiro-Ilan, David I.; Cottrell, Ted E.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Mizell, Russell F., III] Univ Florida, Dept Entomol, Quincy, FL USA. [Horton, Dan L.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Zaid, Abdo] USDA ARS, Off Director Stat, Athens, GA 30613 USA. RP Shapiro-Ilan, DI (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM David.Shapiro@ars.usda.gov FU USDA-NIFA-SARE (Sustainable Agriculture Research and Education) [LS11-241] FX We thank Ryan Burciaga, Leigh Ann Burnell, Stacy Byrd and Kathy Halat, for technical assistance. This study was supported in part by USDA-NIFA-SARE (Sustainable Agriculture Research and Education) Grant No. LS11-241. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the United States Department of Agriculture. NR 42 TC 5 Z9 5 U1 1 U2 31 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2015 VL 82 BP 7 EP 12 DI 10.1016/j.biocontrol.2014.12.003 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CB9OC UT WOS:000349960100002 ER PT J AU Miranda, M Sivinski, J Rull, J Cicero, L Aluja, M AF Miranda, Mario Sivinski, John Rull, Juan Cicero, Lizette Aluja, Martin TI Niche breadth and interspecific competition between Doryctobracon crawfordi and Diachasmimorpha longicaudata (Hymenoptera: Braconidae), native and introduced parasitoids of Anastrepha spp. fruit flies (Diptera: Tephritidae) SO BIOLOGICAL CONTROL LA English DT Article DE Competition; Biological control; Niche overlap; Interspecific interactions ID COMPARATIVE FUNCTIONAL-APPROACH; HOST DETECTION BEHAVIOR; FLY DIPTERA; BIOLOGICAL-CONTROL; LARVAL-PUPAL; BIOSTERES-LONGICAUDATUS; EVOLUTIONARY ARGUMENT; INSECT PARASITOIDS; FIELD EXPERIMENTS; MEXICO AB Interactions among multiple natural enemies can enhance or interfere with their impacts on host/prey populations. Such could be the case with two species of Braconidae that are currently considered for augmentative biological control of pestiferous tephritid fruit flies in Mexico: the exotic Diachasmimorpha longicaudata (Ashmead) and the native Doryctobracon crawfordi (Viereck). Since niche overlap and competition could influence the range and effectiveness of these parasitoids were they to be released together, we compared behaviors and morphologies that might influence their access to hosts. These included ovipositor length, diel pattern of oviposition, effect of host instar on development, host range, host-depth, foraging success in different sized host patches, and effects of super- and multi-parasitism. Intra- and interspecific adult interactions on host patches were also observed. There were significant overlaps in ovipositor length, diel patterns of oviposition, preferred host age, and host depth and size. Doryctobracon crawfordi failed to exploit Anastrepha obliqua and Ceratitis capitata, while Doryctobracon longicaudata parasitized four tephritid species. Doryctobracon longicaudata dominated Doryctobracon crawfordi in multi-parasitism tests and was also better able to survive superparasitism than its competitor. Our results suggest that simultaneous augmentative release of these two species would result in substantial competition. However, because Doryctobracon crawfordi is naturally found at greater densities than Diachasmimorpha longicaudata at high elevations, perhaps because of greater tolerance for cold temperatures, releases in such areas might yield better results than releases of its otherwise superior competitor. (C) 2015 Elsevier Inc. All rights reserved. C1 [Miranda, Mario] Inst Invest Forestales Agr & Pecuarias INIFAP, Apatzingan, Michoacan, Mexico. [Sivinski, John] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32604 USA. [Rull, Juan; Aluja, Martin] Inst Ecol AC, Xalapa 91000, Veracruz, Mexico. [Cicero, Lizette] Inst Nacl Invest Forestales Agr & Pecuarias, Oaxaca, Yucatan, Mexico. RP Aluja, M (reprint author), Inst Ecol AC, Km 2-5 Antigua Carretera Coatepec, Xalapa 91000, Veracruz, Mexico. EM martin.aluja@inecol.mx RI Cicero, Lizette/A-9848-2016 OI Cicero, Lizette/0000-0002-8486-5700 FU Consejo Nacional de Ciencia y Tecnologia (CONACyT); CONACyT [46846-Q]; Campana Nacional contra las Moscas de la Fruta (SAGARPA-IICA-INECOL); United States Department of Agriculture - Agricultural Research Service [USDA-ARS58-6615-3-025]; Sistema Regional de investigacion CONACyT SIGOLFO [96-01003-V]; Instituto de Ecologia (PADEP-UNAM) [030417, 037111]; Instituto de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP) FX This article is part of the Ph.D. dissertation of Mario Miranda directed by Martin Aluja. Mario Miranda acknowledges a fellowship by the Consejo Nacional de Ciencia y Tecnologia (CONACyT). We thank Isabel Jacome, Larissa Guillen, Jaime Pinero, Maurilio Lopez, Astrid Eben, Raquel Cervantes, Andrea Birke, Armando Ibanez, Cesar Ruiz, Braulio Cordoba, Guadalupe Trujillo, Anita Sanchez, Gloria Lagunes, Cecilia Martinez, and Armando Martinez for technical support in Xalapa. We thank Pablo Montoya, Francisco Limon, and Jorge Cancino for support during experiments in Chiapas. We are grateful to two anonymous reviewers for insightful comments on an earlier version of this M. S. This research was funded by CONACyT (46846-Q), Campana Nacional contra las Moscas de la Fruta (SAGARPA-IICA-INECOL), United States Department of Agriculture - Agricultural Research Service (USDA-ARS58-6615-3-025), Sistema Regional de investigacion CONACyT (SIGOLFO 96-01003-V), Instituto de Ecologia (PADEP-UNAM 030417 and 037111), and the Instituto de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP). NR 63 TC 2 Z9 2 U1 3 U2 16 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2015 VL 82 BP 86 EP 95 DI 10.1016/j.biocontrol.2014.12.008 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CB9OC UT WOS:000349960100012 ER PT J AU Diodati, ME Bates, AH Cooley, MB Walker, S Mandrell, RE Brandl, MT AF Diodati, Michelle E. Bates, Anne H. Cooley, Michael B. Walker, Samarpita Mandrell, Robert E. Brandl, Maria T. TI High Genotypic and Phenotypic Similarity Among Shiga Toxin-Producing Escherichia coli O111 Environmental and Outbreak Strains SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID SALMONELLA-TYPHIMURIUM; O26; PREVALENCE; INFECTIONS; ANTIBODIES; CALIFORNIA; CELLULOSE; DIARRHEA; REGION; CATTLE AB Escherichia coli serogroup O111 is among the six most commonly reported non-O157:H7 Shiga toxin-producing E. coli (STEC), which are emerging as important foodborne pathogens. We have assembled a collection of environmental and clinical strains of E. coli O111 from diverse sources and investigated various genotypic and phenotypic characteristics of these strains to gain a better understanding of the epidemiology and biology of this serogroup. Sixty-three percent of the strains (24/38) were of H-type 8, which dominated the environmental- and outbreak-strains group, whereas the sporadic-case strains were more heterogeneous in H-type. All of the environmental and outbreak strains harbored the Shiga toxin 1 gene (stx1), eae, and ehx, and a subset of these also carried the Shiga toxin 2 gene (stx2). Only 9 of 16 sporadic-case strains produced stx1 and/or stx2, and these were mostly of H-type 8 and 10. Pulsed-field gel electrophoresis analysis revealed a cluster of environmental, outbreak, and sporadic illness strains with high phylogenetic similarity. Strains in this pulsogroup were all of the H8 type and STEC pathotype, and carried eae and ehx. Smaller clusters of highly similar STEC O111 strains included outbreak and sporadic illness strains isolated during different time periods or from different geographical locations. A distinct aggregative behavior was observed in the cultures of all environmental and outbreak STEC O111 strains, but not in those of sporadic-case strains. Among environmental and outbreaks strains, aggregation was positively correlated with production of curli fimbriae and RpoS function, and negatively with cellulose synthesis, while the nonaggregative behavior of sporadic-case strains correlated (positively) only with cellulose production. Our results indicate that STEC O111 strains sharing high genotypic similarity and important phenotypic traits with STEC O111 outbreak strains are present in the agricultural environment and may contribute to the burden of foodborne disease. C1 [Diodati, Michelle E.; Bates, Anne H.; Cooley, Michael B.; Walker, Samarpita; Mandrell, Robert E.; Brandl, Maria T.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. RP Brandl, MT (reprint author), ARS, Produce Safety & Microbiol Res Unit, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM maria.brandl@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service CRIS [5325-42000-046, 5325-42000-047] FX The authors thank Yaguang Zhou, Marcia Sousa Oliveira, and Sung Im for technical assistance, and the many people who shared their strains. This research was supported by the United States Department of Agriculture, Agricultural Research Service CRIS projects 5325-42000-046 and 5325-42000-047. NR 38 TC 3 Z9 3 U1 1 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAR 1 PY 2015 VL 12 IS 3 BP 235 EP 243 DI 10.1089/fpd.2014.1887 PG 9 WC Food Science & Technology SC Food Science & Technology GA CD1AI UT WOS:000350805600010 PM 25658825 ER PT J AU Larned, ST Gooseff, MN Packman, AI Rugel, K Wondzell, SM AF Larned, Scott T. Gooseff, Michael N. Packman, Aaron I. Rugel, Kathleen Wondzell, Steven M. TI Groundwater-surface-water interactions: current research directions SO FRESHWATER SCIENCE LA English DT Article ID HYPORHEIC ZONE; ECOLOGY; STREAM C1 [Larned, Scott T.] Natl Inst Water & Atmospher Res, Christchurch, New Zealand. [Gooseff, Michael N.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Packman, Aaron I.] Northwestern Univ, Dept Civil & Environm Engn, Evanston, IL 60208 USA. [Rugel, Kathleen] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Wondzell, Steven M.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP Larned, ST (reprint author), Natl Inst Water & Atmospher Res, Christchurch, New Zealand. EM scott.larned@niwa.co.nz; mgooseff@rams.colostate.edu; a-packman@northwestern.edu; krugel@uga.edu; swondzell@fs.fed.us RI Packman, Aaron/B-7085-2009; Gooseff, Michael/N-6087-2015 OI Gooseff, Michael/0000-0003-4322-8315 NR 30 TC 3 Z9 3 U1 9 U2 54 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2015 VL 34 IS 1 BP 92 EP 98 DI 10.1086/679491 PG 7 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CB9ZV UT WOS:000349995000008 ER PT J AU Zarnetske, JP Haggerty, R Wondzell, SM AF Zarnetske, Jay P. Haggerty, Roy Wondzell, Steven M. TI Coupling multiscale observations to evaluate hyporheic nitrate removal at the reach scale SO FRESHWATER SCIENCE LA English DT Article DE surface-water-groundwater interaction; denitrification; nutrient cycling; nitrogen; residence time ID COASTAL MARINE ECOSYSTEMS; MOUNTAIN STREAM; MASS-TRANSFER; FRESH-WATER; AQUATIC ECOSYSTEMS; HEADWATER STREAMS; SOLUTE TRANSPORT; DENITRIFICATION; DYNAMICS; EXCHANGE AB Excess NO3- in streams is a growing and persistent problem for both inland and coastal ecosystems, and denitrification is the primary removal process for NO3-. Hyporheic zones can have high denitrification potentials, but their role in reach- and network-scale NO3- removal is unknown because it is difficult to estimate. We used independent and complementary multiscale measurements of denitrification and total NO3- uptake to quantify the role of hyporheic NO3- removal in a 303-m reach of a 3(rd)-order agricultural stream in western Oregon, USA. We characterized the reach-scale NO3- dynamics with steady-state N-15-NO3- tracer-addition experiments and solute-transport modeling, and measured the hyporheic conditions via in-situ biogeochemical and groundwater modeling. We also developed a method to link these independent multiscale measurements. Hyporheic NO3- removal (rate coefficient (HZ) = 0.007/h) accounted for 17% of the observed total reach NO3- uptake and 32% of the reach denitrification estimated from the N-15 experiments. The primary limitations on hyporheic denitrification at the reach scale were availability of labile dissolved organic C and the restricted size of the hyporheic zone caused by anthropogenic channelization (sediment thickness 1.5 m). Linking multiscale methods made estimates possible for hyporheic influence on stream NO3- dynamics. However, it also demonstrated that the traditional reach-scale tracer experimental designs and subsequent transport modeling cannot be used alone to directly investigate the role of the hyporheic zone on reach-scale water and solute dynamics. C1 [Zarnetske, Jay P.] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48824 USA. [Haggerty, Roy] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Haggerty, Roy] Oregon State Univ, Inst Water & Watersheds, Corvallis, OR 97331 USA. [Wondzell, Steven M.] US Forest Serv, Pacific NW Res Stn, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. RP Zarnetske, JP (reprint author), Michigan State Univ, Dept Geol Sci, E Lansing, MI 48824 USA. EM jpz@cns.msu.edu; haggertr@geo.oregonstate.edu; swondzell@fs.fed.us RI Haggerty, Roy/A-5863-2009; Zarnetske, Jay/F-4685-2010 OI Zarnetske, Jay/0000-0003-2777-5378 FU National Science Foundation (NSF) [DGE-0333257, EAR-0409534]; Oregon State University (OSU) Institute for Water and Watersheds (IWW); Yale Institute for Biospheric Studies; Hollis M. Dole Environmental Geology Foundation at Oregon State University FX This research was supported by National Science Foundation (NSF) grants DGE-0333257 and EAR-0409534 and an Oregon State University (OSU) Institute for Water and Watersheds (IWW) grant. Further support was provided by the Yale Institute for Biospheric Studies and the Hollis M. Dole Environmental Geology Foundation at Oregon State University. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the NSF. We thank the Harry Klopfenstein Farm for generously granting access to the Drift Creek research sites. We are very appreciative of the important role Associate Editor Aaron Packman and referees played in improving this manuscript. We extend special thanks to: V. Adams, S. Baxter, P. Zarnetske, A. Argerich, and B. Burkholder for field/laboratory assistance; L. Ashkenas and S. Thomas for advising JPZ on stable 15N handling; M. Otter of MBL's Stable Isotope Laboratory for analyzing 15N samples; and C. Jones and K. Motter of Cooperative Chemical Analytical Laboratory and OSU IWW Collaboratory for help with analyzing general water chemistry. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the US Department of Agriculture of any product or service. NR 60 TC 5 Z9 5 U1 3 U2 50 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2015 VL 34 IS 1 BP 172 EP 186 DI 10.1086/680011 PG 15 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CB9ZV UT WOS:000349995000014 ER PT J AU Wondzell, SM AF Wondzell, Steven M. TI Groundwater-surface-water interactions: perspectives on the development of the science over the last 20 years SO FRESHWATER SCIENCE LA English DT Article DE hyporheic; groundwater; streams; conceptual models; literature review ID HYPORHEIC ZONE; INTEGRATING HYDROLOGY; TRANSIENT STORAGE; SOLUTE TRANSPORT; 3RD-ORDER STREAM; RIVER CONTINUUM; POROUS-MEDIA; REACH-SCALE; FLOW; DYNAMICS AB Freshwater Science published a special series of papers on groundwater-surface-water (GW-SW) interactions in this issue (2015), marking the anniversary of an earlier special series of papers on the hyporheic zone published in 1993. In this concluding paper, I compare the 2 special series of papers and use this comparison to examine the development of the science in the years between 1993 and 2015. The 1993 papers marked the beginning of a period of exponential growth in the study of, and publication of, papers on GW-SW interactions. The 1993 papers tended to be forward looking, proposing conceptual models of GW-SW interactions across stream networks and identifying critical gaps. The 2015 special series of papers contrasts sharply with that of 1993. Broad issue papers are mostly lacking from the current special series. Instead, the special series is dominated by papers focusing on process-based or descriptive studies using empirical approaches. This difference probably stems from major methodological advancements over the past 2 decades that make it possible to study GW-SW interactions with ever greater detail and, thus, allows more complete understanding of specific processes. In contrast, the authors of the 1993 special series were acutely aware of the paucity of GW-SW studies and, thus, posed their conceptual models as hypotheses. Surprisingly, these hypotheses have not been rigorously tested in the decades since their publication. Perhaps it is time to re-examine such broad conceptual models. There remains a critical need for a holistic understanding of how GW-SW interactions vary among streams types and sizes and with changes in discharge among seasons or over storm events and how these GW-SW interactions influence stream ecosystem processes. C1 US Forest Serv, Pacific NW Res Stn, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. RP Wondzell, SM (reprint author), US Forest Serv, Pacific NW Res Stn, Corvallis Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM swondzell@fs.fed.us NR 58 TC 2 Z9 2 U1 3 U2 49 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD MAR PY 2015 VL 34 IS 1 BP 368 EP 376 DI 10.1086/679665 PG 9 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CB9ZV UT WOS:000349995000028 ER PT J AU Chu, HS Gottgens, JF Chen, JQ Sun, G Desai, AR Ouyang, ZT Shao, CL Czajkowski, K AF Chu, Housen Gottgens, Johan F. Chen, Jiquan Sun, Ge Desai, Ankur R. Ouyang, Zutao Shao, Changliang Czajkowski, Kevin TI Climatic variability, hydrologic anomaly, and methane emission can turn productive freshwater marshes into net carbon sources SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon budget; carbon dioxide; carbon sequestration; dissolved organic carbon; eddy-covariance; methane; particulate organic carbon ID ENERGY-BALANCE CLOSURE; ECOSYSTEM CO2 EXCHANGE; ORGANIC-CARBON; NUTRIENT ACCUMULATION; LAKE-ERIE; EDDY COVARIANCE; INTERANNUAL VARIABILITY; PHRAGMITES-AUSTRALIS; PRAIRIE WETLAND; FLOATING LEAVES AB Freshwater marshes are well-known for their ecological functions in carbon sequestration, but complete carbon budgets that include both methane (CH4) and lateral carbon fluxes for these ecosystems are rarely available. To the best of our knowledge, this is the first full carbon balance for a freshwater marsh where vertical gaseous [carbon dioxide (CO2) and CH4] and lateral hydrologic fluxes (dissolved and particulate organic carbon) have been simultaneously measured for multiple years (2011-2013). Carbon accumulation in the sediments suggested that the marsh was a long-term carbon sink and accumulated similar to 96.9 +/- 10.3 (+/- 95% CI) g Cm(-2)yr(-1) during the last similar to 50years. However, abnormal climate conditions in the last 3years turned the marsh to a source of carbon (42.7 +/- 23.4g Cm(-2)yr(-1)). Gross ecosystem production and ecosystem respiration were the two largest fluxes in the annual carbon budget. Yet, these two fluxes compensated each other to a large extent and led to the marsh being a CO2 sink in 2011 (-78.8 +/- 33.6g Cm(-2)yr(-1)), near CO2-neutral in 2012 (29.7 +/- 37.2g C m(-2)yr(-1)), and a CO2 source in 2013 (92.9 +/- 28.0g Cm(-2)yr(-1)). The CH4 emission was consistently high with a three-year average of 50.8 +/- 1.0g Cm(-2)yr(-1). Considerable hydrologic carbon flowed laterally both into and out of the marsh (108.3 +/- 5.4 and 86.2 +/- 10.5g Cm(-2)yr(-1), respectively). In total, hydrologic carbon fluxes contributed similar to 23 +/- 13g Cm(-2)yr(-1) to the three-year carbon budget. Our findings highlight the importance of lateral hydrologic inflows/outflows in wetland carbon budgets, especially in those characterized by a flow-through hydrologic regime. In addition, different carbon fluxes responded unequally to climate variability/anomalies and, thus, the total carbon budgets may vary drastically among years. C1 [Chu, Housen; Gottgens, Johan F.; Chen, Jiquan; Ouyang, Zutao; Shao, Changliang] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC USA. [Desai, Ankur R.] Univ Wisconsin, Dept Atmospher & Ocean Sci, Madison, WI USA. [Shao, Changliang] Univ Toledo, Lake Erie Ctr, Oregon, OH USA. [Czajkowski, Kevin] Univ Toledo, Dept Geog & Planning, Toledo, OH 43606 USA. RP Chu, HS (reprint author), Univ Toledo, Dept Environm Sci, 2801 W Bancroft St, Toledo, OH 43606 USA. EM hchu6@rockets.utoledo.edu RI Desai, Ankur/A-5899-2008; Chu, Housen/Q-6517-2016; Chen, Jiquan/D-1955-2009 OI Desai, Ankur/0000-0002-5226-6041; Chu, Housen/0000-0002-8131-4938; FU National Oceanic and Atmospheric Administration (NOAA) [NA10OAR4170224]; National Science Foundation (NSF) [NSF1034791, NSF0845166] FX This project was funded by the National Oceanic and Atmospheric Administration (NOAA) (NA10OAR4170224) and the National Science Foundation (NSF) (NSF1034791) Field Station and Marine Labs (FSML) program, USA. ARD was supported by NSF (NSF0845166). We thank John Simpson and the Winous Point Marsh Conservancy for fully supporting the research platform and logistical assistance at the Winous Point North Marsh. We thank Richard Becker, Timothy Fisher, James Martin-Hayden, Donald Cahoon, Karen Roderick-Lingema, Asko Noormets, Thomas Bridgeman, Kristin Kirschbaum, Ranjeet John, Barry Muller, Jeremy Pritt, Walter Berger, and Haiqiang Guo for their helpful assistance and advice. We thank the Subject Editor and three anonymous reviewers for providing valuable suggestions for the quality of the study. We gratefully acknowledge Mike Deal, Jianye Xu, Orrin Babcock, Cory Becher, Yahn-Jauh Su, Jing Xie, Terenzio Zenone, and Jennifer Teeple for building and maintaining the site infrastructure and assisting in the data collection and management. We also thank Lisa Delp Taylor for editing the manuscript. NR 99 TC 9 Z9 9 U1 5 U2 71 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2015 VL 21 IS 3 BP 1165 EP 1181 DI 10.1111/gcb.12760 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CB8QS UT WOS:000349896400014 PM 25287051 ER PT J AU Petrie, MD Collins, SL Swann, AM Ford, PL Litvak, ME AF Petrie, M. D. Collins, S. L. Swann, A. M. Ford, P. L. Litvak, M. E. TI Grassland to shrubland state transitions enhance carbon sequestration in the northern Chihuahuan Desert SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon sequestration; Chihuahuan Desert; creosotebush shrubland; desert grassland; ecological state transition; ecosystem services ID WOODY PLANT INVASION; CENTRAL NEW-MEXICO; GLOBAL DESERTIFICATION; AMERICAN GRASSLANDS; SEMIARID ECOSYSTEMS; CLIMATE VARIABILITY; PULSE DYNAMICS; UNITED-STATES; ENCROACHMENT; MONSOON AB The replacement of native C-4-dominated grassland by C-3-dominated shrubland is considered an ecological state transition where different ecological communities can exist under similar environmental conditions. These state transitions are occurring globally, and may be exacerbated by climate change. One consequence of the global increase in woody vegetation may be enhanced ecosystem carbon sequestration, although the responses of arid and semiarid ecosystems may be highly variable. During a drier than average period from 2007 to 2011 in the northern Chihuahuan Desert, we found established shrubland to sequester 49g Cm(-2)yr(-1) on average, while nearby native C-4 grassland was a net source of 31g Cm(-2)yr(-1) over this same period. Differences in C exchange between these ecosystems were pronounced - grassland had similar productivity compared to shrubland but experienced higher C efflux via ecosystem respiration, while shrubland was a consistent C sink because of a longer growing season and lower ecosystem respiration. At daily timescales, rates of carbon exchange were more sensitive to soil moisture variation in grassland than shrubland, such that grassland had a net uptake of C when wet but lost C when dry. Thus, even under unfavorable, drier than average climate conditions, the state transition from grassland to shrubland resulted in a substantial increase in terrestrial C sequestration. These results illustrate the inherent tradeoffs in quantifying ecosystem services that result from ecological state transitions, such as shrub encroachment. In this case, the deleterious changes to ecosystem services often linked to grassland to shrubland state transitions may at least be partially offset by increased ecosystem carbon sequestration. C1 [Petrie, M. D.; Collins, S. L.; Litvak, M. E.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Swann, A. M.] Univ New Mexico, Dept Biol, Sevilleta LTER, Albuquerque, NM 87131 USA. [Ford, P. L.] US Forest Serv, USDA, Rocky Mt Res Stn, Albuquerque, NM USA. RP Petrie, MD (reprint author), Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. EM matt@sevilleta.unm.edu RI Collins, Scott/P-7742-2014 OI Collins, Scott/0000-0002-0193-2892 FU National Science Foundation; USDA Forest Service, Western Wildland Environmental Threat Assessment Center; NASA [NNX11AG91G]; NSF-EPSCoR award for Hydrology FX This research was conducted using four datasets collected through the Sevilleta LTER. This research was partially funded by a National Science Foundation grant to the University of New Mexico for Long Term Ecological Research, by a grant from the USDA Forest Service, Western Wildland Environmental Threat Assessment Center, by NASA grant award NNX11AG91G to Litvak, and through an NSF-EPSCoR award for Hydrology to the University of New Mexico. NR 63 TC 7 Z9 7 U1 13 U2 105 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAR PY 2015 VL 21 IS 3 BP 1226 EP 1235 DI 10.1111/gcb.12743 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CB8QS UT WOS:000349896400018 PM 25266205 ER PT J AU Stewart, CE Follett, RF Pruessner, EG Varvel, GE Vogel, KP Mitchell, RB AF Stewart, Catherine E. Follett, Ronald F. Pruessner, Elizabeth G. Varvel, Gary E. Vogel, Kenneth P. Mitchell, Robert B. TI Nitrogen and harvest effects on soil properties under rainfed switchgrass and no-till corn over 9 years: implications for soil quality SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE harvest timing; no-till corn; P, K, N fertilizer; residue removal; soil C sequestration; soil organic C; switchgrass ID ORGANIC-MATTER DYNAMICS; CARBON SEQUESTRATION; BIOMASS PRODUCTION; MICROBIAL RESPONSE; CROPPING SYSTEMS; CENTRAL IOWA; USA; MANAGEMENT; BIOENERGY; PHOSPHORUS AB Nitrogen fertilizer and harvest management will alter soils under bioenergy crop production and the long-term effects of harvest timing and residue removal remain relatively unknown. Compared to no-tilled corn (NT-C, Zea mays L.), switchgrass (Panicum virgatum L.) is predicted to improve soil properties [i.e. soil organic C (SOC), soil microbial biomass (SMB-C), and soil aggregation] due to its perennial nature and deep-rooted growth form, but few explicit field comparisons exist. We assessed soil properties over 9 years for a rainfed study of N fertilizer rate (0, 60, 120, and 180 kg N ha(-1)) and harvest management on switchgrass (harvested in August and postfrost) and NT-C (with and without 50% stover removal) in eastern NE. We measured SOC, aggregate stability, SMB-C, bulk density (BD), pH, P and K in the top 0-30 cm. Both NT-C and switchgrass increased SMB-C, SOC content, and aggregate stability over the 9 years, reflecting improvement from previous conventional management. However, the soils under switchgrass had double the percent aggregate stability, 1.3 times more microbial biomass, and a 5-8% decrease in bulk density in the 0-5 and 5-10 cm depths compared to NT-C. After 9 years, cumulative decrease in available P was significantly greater beneath NT-C (-24.0 kg P ha(-1)) compared to switchgrass (-5.4 kg P ha(-1)). When all measured soil parameters were included in the Soil Management Assessment Framework (SMAF), switchgrass improved soil quality index over time (Delta SQI) in all depths. NT-C without residue removal did not affect Delta SQI, but 50% residue removal decreased Delta SQI (0-30 cm) due to reduced aggregate stability and SMB-C. Even with best-management practices such as NT, corn stover removal will have to be carefully managed to prevent soil degradation. Long-term N and harvest management studies that include biological, chemical, and physical soil measurements are necessary to accurately assess bioenergy impacts on soils. C1 [Stewart, Catherine E.; Follett, Ronald F.; Pruessner, Elizabeth G.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. [Varvel, Gary E.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Vogel, Kenneth P.; Mitchell, Robert B.] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Stewart, CE (reprint author), USDA ARS, Soil Plant Nutrient Res Unit, Suite 100,2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM catherine.stewart@colostate.edu OI Stewart, Catherine/0000-0003-1216-0450 FU US Department of Agriculture (USDA), Agricultural Research Service (ARS); USDA Natural Resources Conservation Service FX This research was funded by the US Department of Agriculture (USDA), Agricultural Research Service (ARS), and the USDA Natural Resources Conservation Service. The authors acknowledge the important technical contributions of Edward Buenger, Susan Sampson-Liebig, Steven Masterson, Patrick Callahan, Kevin Grams, Stephan Swanson, and many student employees. The field assistance and coordination by Steve Scheinost, Asst. State Soil Scientist, USDA, NRCS, for Nebraska and laboratory support provided by the USDA, NRCS, Soil Survey Laboratory (National Soil Survey Center) is acknowledged. This work is part of the USDA-ARS GRACEnet network. NR 54 TC 13 Z9 13 U1 6 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD MAR PY 2015 VL 7 IS 2 BP 288 EP 301 DI 10.1111/gcbb.12142 PG 14 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CB8QI UT WOS:000349895300012 ER PT J AU de Figueiredo, EB Panosso, AR Reicosky, DC La Scala, N AF de Figueiredo, Eduardo B. Panosso, Alan R. Reicosky, Donald C. La Scala, Newton, Jr. TI Short-term CO2-C emissions from soil prior to sugarcane (Saccharum spp.) replanting in southern Brazil SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE agriculture; crop straw; dolomite; greenhouse gas emissions; harvest systems; mitigation strategies ID CARBON-DIOXIDE EMISSIONS; GREENHOUSE-GAS BALANCE; PICEA-ABIES L; ETHANOL-PRODUCTION; WATER CONTENT; UNITED-STATES; TILLAGE; RESPIRATION; SEQUESTRATION; MANAGEMENT AB New management strategies should be identified to increase the potential of bioenergy crops to minimize climate change. This study quantified the impact of sugarcane (Saccharum spp.) harvest systems, straw and soil management on carbon dioxide (CO2) fluxes prior to crop replanting carried out on February 2010 in southern Brazil. The soil studied was classified as Haplustult (USDA Soil Taxonomy). Three sugarcane harvest systems were considered: burned (BH) and green harvest with straw maintained on (GH SM) or removed from (GH SR) the soil surface. Our hypothesis is that intensive tillage and the management of sugarcane crop straw could lead to higher CO2 emissions from soil. We measured CO2 emissions in no-till (NT) conditions and after conventional tillage (CT), and with or without dolomite and agricultural gypsum applications. Soil CO2 emissions were measured with a Li Cor chamber (Model Li-8100). Water content of soil and soil temperature readings were first taken 24 h after tillage, over the next 25 days after tillage with 18 measurement days. The removal of sugarcane straw from the soil surface resulted in the rapid reduction of water content of soil (6% in volume) followed by a 64% increase in soil CO2-C emissions, supporting our hypothesis. Additional soil CO2-C emissions caused by removal of crop straw were 253 kg CO2-C ha(-1), which is as high as CO2-C losses induced by tillage. Dolomite and agricultural gypsum applications did not always increase CO2 emissions, especially when applied on soil surface with crop straw and tilled. The conversion from burned to green harvest systems can improve the soil C sequestration rate in sugarcane crops when combined with reduced tillage and straw maintenance on soil surface. The effect of straw removal and related CO2 emission for electricity generation should be considered in further studies from sugarcane areas. C1 [de Figueiredo, Eduardo B.; Panosso, Alan R.; La Scala, Newton, Jr.] Univ Estadual Paulista, FCAV, Dept Ciencias Exatas, Sao Paulo, Brazil. [Reicosky, Donald C.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP de Figueiredo, EB (reprint author), Univ Estadual Paulista, FCAV, Dept Ciencias Exatas, Sao Paulo, Brazil. EM eduardobfigueiredo@hotmail.com RI La Scala Jr., Newton/C-4398-2012 FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); Usina Ipiranga de Acucar e Alcool S.A. FX We are grateful to Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), and Usina Ipiranga de Acucar e Alcool S.A., for support. NR 76 TC 0 Z9 0 U1 1 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD MAR PY 2015 VL 7 IS 2 BP 316 EP 327 DI 10.1111/gcbb.12151 PG 12 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CB8QI UT WOS:000349895300014 ER PT J AU Hoover, CM Heath, LS AF Hoover, Coeli M. Heath, Linda S. TI A commentary on 'Mineral soil carbon fluxes in forests and implications for carbon balance assessments': a deeper look at the data SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Editorial Material DE carbon accounting; carbon assessments; forest soil; management impacts; soil organic carbon; forest soil ID LAND-USE CHANGE; META ANALYSIS; STORAGE; CHRONOSEQUENCE; MANAGEMENT; STOCKS AB Forestry practitioners contacted us with their concerns about a recent review article by Buchholz T, Friedland AJ, Hornig CE, Keeton WS, Zanchi G, Nunery J (2013) GCB Bioenergy who questioned the way soil carbon is treated in many models and protocols, and indicated that an increasing number of research studies showed meaningful soil organic carbon (SOC) loss as a result of forest management. We revisit the major studies cited in the review and present a more complete look at the results, consistently treat forest floor carbon as a separate pool, discuss differences in interpretation, and suggest opportunities to advance the state of knowledge regarding SOC and forest carbon accounting. Overall, we conclude that the literature continues to support the current default assumption of little or no change in mineral SOC when sound forest management practices are followed. C1 [Hoover, Coeli M.; Heath, Linda S.] USDA Forest Serv, No Res Stn, Durham, NH 03824 USA. RP Hoover, CM (reprint author), USDA Forest Serv, No Res Stn, Durham, NH 03824 USA. EM choover@fs.fed.us NR 18 TC 1 Z9 1 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD MAR PY 2015 VL 7 IS 2 BP 362 EP 365 DI 10.1111/gcbb.12130 PG 4 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CB8QI UT WOS:000349895300018 ER PT J AU Sanchez-Murillo, R Brooks, ES Elliot, WJ Gazel, E Boll, J AF Sanchez-Murillo, R. Brooks, E. S. Elliot, W. J. Gazel, E. Boll, J. TI Baseflow recession analysis in the inland Pacific Northwest of the United States SO HYDROGEOLOGY JOURNAL LA English DT Article DE USA; Groundwater/surface-water relations; Geology; Climate; Watershed management ID CLIMATE-CHANGE; STREAM TEMPERATURE; HUMID REGIONS; FLOW-ANALYSIS; TRENDS; BASIN; VARIABILITY; CATCHMENTS; HYDROLOGY; IMPACTS AB The storage-discharge relationships of 26 watersheds in the inland Pacific Northwest of the United States were analyzed. Four fitting methods were used to obtain the baseflow coefficients: lower envelope, organic correlation, and ordinary and inverse least squares. Several climatic and terrain attributes were evaluated as predictors of baseflow coefficients. Watersheds dominated by basalt and flatter landscapes exhibited the smallest recession time scales (K) (12.5-20.0 days). Greater K values (33.3-66.7 days) were obtained over catchments dominated by metamorphic and sedimentary rocks. Mean basin slope and the aridity index were found to be the best estimators of baseflow coefficients. Baseflow in flat basalt landscapes, located in dry warm climates, decrease rapidly during summer months and are most sensitive to future droughts and warming climates. Groundwater systems feeding streams during the driest months can drop to less than 1 mm of effective storage in these sensitive systems. In contrast, the minimum annual storage in mountainous systems can have greater than 10 mm effective storage. By understanding the main factors controlling baseflow recession characteristics, environmental agencies could prioritize efforts in areas where future droughts and land use changes may affect ecological assemblages and socio-economic activities. C1 Univ Idaho, W Water Resources Program, Waters, Moscow, ID 83844 USA. [Sanchez-Murillo, R.; Boll, J.] Univ Idaho, West Water Resources Program, Moscow, ID 83844 USA. [Sanchez-Murillo, R.] Univ Nacl, Dept Chem, Heredia, Costa Rica. [Brooks, E. S.; Boll, J.] Univ Idaho, Dept Biol & Agr Engn, Moscow, ID 83844 USA. [Elliot, W. J.] USDA Forest Serv Rocky Mt Res Stn, Moscow, ID 83843 USA. [Gazel, E.] Virginia Tech, Dept Geosci, Blacksburg, VA 24061 USA. RP Sanchez-Murillo, R (reprint author), Univ Nacl, Dept Chem, POB 86-3000, Heredia, Costa Rica. EM sanc7767@vandals.uidaho.edu FU USDA-Forest Service Rocky Mountain Research Station [10-JV-11221634-252]; University of Idaho [10-JV-11221634-252] FX This project was funded by the joint venture agreement (No. 10-JV-11221634-252) between USDA-Forest Service Rocky Mountain Research Station and the University of Idaho. The authors thank the insights and useful comments from two anonymous reviewers. NR 45 TC 3 Z9 4 U1 1 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1431-2174 EI 1435-0157 J9 HYDROGEOL J JI Hydrogeol. J. PD MAR PY 2015 VL 23 IS 2 BP 287 EP 303 DI 10.1007/s10040-014-1191-4 PG 17 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA CC0PO UT WOS:000350038200007 ER PT J AU Letcher, BH Schueller, P Bassar, RD Nislow, KH Coombs, JA Sakrejda, K Morrissey, M Sigourney, DB Whiteley, AR O'Donnell, MJ Dubreuil, TL AF Letcher, Benjamin H. Schueller, Paul Bassar, Ronald D. Nislow, Keith H. Coombs, Jason A. Sakrejda, Krzysztof Morrissey, Michael Sigourney, Douglas B. Whiteley, Andrew R. O'Donnell, Matthew J. Dubreuil, Todd L. TI Robust estimates of environmental effects on population vital rates: an integrated capture-recapture model of seasonal brook trout growth, survival and movement in a stream network SO JOURNAL OF ANIMAL ECOLOGY LA English DT Article DE annual survival; Bayesian modelling; capture-mark-recapture; integrated model; movement; sensitivity; stream fish; stream network; survival ID AGE-STRUCTURED POPULATION; SALMON SALMO-SALAR; JOLLY-SEBER MODEL; ATLANTIC SALMON; MARK-RECAPTURE; SALVELINUS-FONTINALIS; SENSITIVITY-ANALYSIS; VIABILITY ANALYSIS; PROJECTION MODELS; UNIFIED APPROACH AB 1. Modelling the effects of environmental change on populations is a key challenge for ecologists, particularly as the pace of change increases. Currently, modelling efforts are limited by difficulties in establishing robust relationships between environmental drivers and population responses. We developed an integrated capture-recapture state-space model to estimate the effects of two key environmental drivers (stream flow and temperature) on demographic rates (body growth, movement and survival) using a long-term (11years), high-resolution (individually tagged, sampled seasonally) data set of brook trout (Salvelinus fontinalis) from four sites in a stream network. Our integrated model provides an effective context within which to estimate environmental driver effects because it takes full advantage of data by estimating (latent) state values for missing observations, because it propagates uncertainty among model components and because it accounts for the major demographic rates and interactions that contribute to annual survival. We found that stream flow and temperature had strong effects on brook trout demography. Some effects, such as reduction in survival associated with low stream flow and high temperature during the summer season, were consistent across sites and age classes, suggesting that they may serve as robust indicators of vulnerability to environmental change. Other survival effects varied across ages, sites and seasons, indicating that flow and temperature may not be the primary drivers of survival in those cases. Flow and temperature also affected body growth rates; these responses were consistent across sites but differed dramatically between age classes and seasons. Finally, we found that tributary and mainstem sites responded differently to variation in flow and temperature. Annual survival (combination of survival and body growth across seasons) was insensitive to body growth and was most sensitive to flow (positive) and temperature (negative) in the summer and fall. These observations, combined with our ability to estimate the occurrence, magnitude and direction of fish movement between these habitat types, indicated that heterogeneity in response may provide a mechanism providing potential resilience to environmental change. Given that the challenges we faced in our study are likely to be common to many intensive data sets, the integrated modelling approach could be generally applicable and useful. C1 [Letcher, Benjamin H.; Schueller, Paul; Bassar, Ronald D.; Sakrejda, Krzysztof; Morrissey, Michael; Sigourney, Douglas B.; O'Donnell, Matthew J.; Dubreuil, Todd L.] US Geol Survey, SO Conte Anadromous Fish Res Ctr, Leetown Sci Ctr, Turners Falls, MA 01376 USA. [Schueller, Paul; Sakrejda, Krzysztof] Univ Massachusetts, Program Organism & Evolutionary Biol, Amherst, MA 01003 USA. [Nislow, Keith H.; Coombs, Jason A.] Univ Massachusetts, USDA Forest Serv, Northern Res Stn, Amherst, MA 01003 USA. [Morrissey, Michael] Univ St Andrews, Sch Biol, St Andrews KY16 9ST, Fife, Scotland. [Whiteley, Andrew R.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. RP Letcher, BH (reprint author), US Geol Survey, SO Conte Anadromous Fish Res Ctr, Leetown Sci Ctr, Turners Falls, MA 01376 USA. EM bletcher@usgs.gov RI Morrissey, Michael/F-3226-2010 OI Morrissey, Michael/0000-0001-6209-0177 FU US Fish and Wildlife Service North Atlantic Conservation Cooperative; National Institute of Food and Agriculture, U.S. Department of Agriculture; Massachusetts Agricultural Experiment Station; Environmental Conservation Department of the University of Massachusetts Amherst [14] FX Many thanks to all the students and interns who have helped sample the West Brook. Partial funding was provided by the US Fish and Wildlife Service North Atlantic Conservation Cooperative and the National Institute of Food and Agriculture, U.S. Department of Agriculture, the Massachusetts Agricultural Experiment Station and the Environmental Conservation Department of the University of Massachusetts Amherst, under project number MAS # 14. Thanks also to Jeff Falgout of the USGS Core Science Analytics and Synthesis Center in Denver CO, USA, for facilitating computer runs on the USGS computer cluster in Denver. We thank two anonymous reviewers, Dan Hocking and Will Fields, for helpful comments on a manuscript draft. NR 62 TC 10 Z9 10 U1 7 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8790 EI 1365-2656 J9 J ANIM ECOL JI J. Anim. Ecol. PD MAR PY 2015 VL 84 IS 2 BP 337 EP 352 DI 10.1111/1365-2656.12308 PG 16 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CB9RB UT WOS:000349968700003 PM 25327608 ER PT J AU Huang, XF Zhou, D Guo, J Manter, DK Reardon, KF Vivanco, JM AF Huang, X-F. Zhou, D. Guo, J. Manter, D. K. Reardon, K. F. Vivanco, J. M. TI Bacillus spp. from rainforest soil promote plant growth under limited nitrogen conditions SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Bacillus atrophaeus; Bacillus pumilus; Bacillus subtilis; induced systemic resistance; plant growth-promoting rhizobacteria; rainforest soil ID PSEUDOMONAS-CHLORORAPHIS O6; INDUCED SYSTEMIC RESISTANCE; ARABIDOPSIS-THALIANA; DEPENDENT PRODUCTION; RHIZOBACTERIA; BACTERIA; MICROORGANISMS; CUCUMBER; ROOT; BIODIVERSITY AB AimsThe aim of this study was to evaluate effects of PGPR (plant growth-promoting rhizobacteria) isolated from rainforest soil on different plants under limited nitrogen conditions. Methods and ResultsBacterial isolates from a Peruvian rainforest soil were screened for plant growth-promoting effects on Arabidopsis (Col-0). Four selected isolates including one Bacillus subtilis, two B. atrophaeus and one B.pumilus significantly promoted growth of Zea mays L. and Solanum lycopersicum under greenhouse conditions. Moreover, the PGPRs significantly promoted growth of S.lycopersicum in both low and nitrogen-amended soil conditions. These PGPR strains were further studied to obtain insights into possible mechanisms of plant growth promotion. Volatile chemicals from those isolates promoted Arabidopsis growth, and the expression of genes related to IAA production was induced in the Arabidopsis plants treated with PGPRs. Further, selected PGPR strains triggered induced systemic resistance (ISR) against Pseudomonas syringae pv tomato DC3000 in Arabidopsis. ConclusionsPGPR strains isolated from the rainforest soil promoted the plant growth of Arabidopsis, corn and tomato. Significance and Impact of the StudyNew PGPR that have wider adaptability to different crops, soils and environmental conditions are needed to decrease our reliance on agricultural amendments derived from fossil-based fuels. The PGPRs isolated from a nonagricultural site constitute new plant growth-promoting strains that could be developed for agricultural uses. C1 [Huang, X-F.; Zhou, D.; Vivanco, J. M.] Colorado State Univ, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA. [Huang, X-F.; Reardon, K. F.] Colorado State Univ, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA. [Zhou, D.; Guo, J.] Nanjing Agr Univ, Dept Plant Pathol, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China. [Manter, D. K.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80522 USA. [Reardon, K. F.; Vivanco, J. M.] Colorado State Univ, Cell & Mol Biol Grad Program, Ft Collins, CO 80523 USA. RP Vivanco, JM (reprint author), Colorado State Univ, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA. EM j.vivanco@colostate.edu RI Reardon, Kenneth/A-1952-2016 OI Reardon, Kenneth/0000-0002-7753-4049 NR 55 TC 1 Z9 4 U1 0 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAR PY 2015 VL 118 IS 3 BP 672 EP 684 DI 10.1111/jam.12720 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CB9TR UT WOS:000349976400014 PM 25494882 ER PT J AU Wiman, NG Walton, VM Shearer, PW Rondon, SI Lee, JC AF Wiman, Nik G. Walton, Vaughn M. Shearer, Peter W. Rondon, Silvia I. Lee, Jana C. TI Factors affecting flight capacity of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) SO JOURNAL OF PEST SCIENCE LA English DT Article DE Flight mill; Dispersal; Invasive species; BMSB ID LIFE TABLE ANALYSIS; APHIS FABAE SCOP; STAL HETEROPTERA; TETHERED FLIGHT; CUTICULAR HYDROCARBONS; PHYSIOLOGICAL FACTORS; MATED STATUS; WATER-LOSS; TEMPERATURE; LEPIDOPTERA AB Brown marmorated stink bug, Halyomorpha halys (StAyenl) is a highly destructive invasive pest of annual and perennial crops in the eastern United States and is an increasing threat to agriculture in the Pacific Northwest. Flight mills were used to examine flight capacity of H. halys in order to better understand its invasive characteristics. Specifically, we examined generational, sexual, and phenotypical effects on flight distance, frequency, velocity, and diel flight patterns of field-collected H. halys from two seasons. There was a clear dichotomy in total flight distance for insects that flew 5 km or less and those that flew more than 5 km in 24 h. The tendency for long distance flight of H. halys changed over the course of a given season, peaking at the end of the growing season. Summer generation H. halys flew farther and faster than overwintered adults, but not as frequently. Males and females had similar numbers of flights, but females went farther. Pre-flight weight of insects was correlated flight activity of adults in terms of speed, frequency, and distance. Overwintered H. halys lost a greater proportion of their pre-flight body weight during the assay than did the summer generation adults. Despite many limitations extrapolating flight mill data to the field, this study nonetheless provided evidence that H. halys has the capacity for long distance flight, particularly in the summer generation. The nutritional status and fat reserves of overwintered versus summer generation adults are discussed as potential explanations for the flight patterns found in this study. C1 [Wiman, Nik G.; Walton, Vaughn M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Shearer, Peter W.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, Hood River, OR 97031 USA. [Rondon, Silvia I.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR USA. [Lee, Jana C.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR USA. RP Wiman, NG (reprint author), Oregon State Univ, Dept Hort, Agr & Life Sci Bldg 4017, Corvallis, OR 97331 USA. EM nik.wiman@oregonstate.edu FU Agriculture and Food Research Initiative Specialty Crop Research Initiative from the USDA National Institute of Food and Agriculture [2011-51181-30937] FX We thank Wyatt Rice-Narusch for help with flight mill assembly, Erika Maslen and Chris Hedstrom for assisting with field collections, and Teah Smith for technical assistance with the flight mills. Thanks to Doo-Hyung Lee for comments that improved this manuscript. This research was supported by Agriculture and Food Research Initiative Specialty Crop Research Initiative Competitive Grant no. 2011-51181-30937 from the USDA National Institute of Food and Agriculture. NR 59 TC 19 Z9 19 U1 10 U2 54 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD MAR PY 2015 VL 88 IS 1 BP 37 EP 47 DI 10.1007/s10340-014-0582-6 PG 11 WC Entomology SC Entomology GA CC0PW UT WOS:000350039000005 ER PT J AU Fontenot, EA Arthur, FH Hartzer, KL AF Fontenot, Emily A. Arthur, Frank H. Hartzer, Kris L. TI Effect of diet and refugia on development of Dermestes maculatus DeGeer reared in a laboratory SO JOURNAL OF PEST SCIENCE LA English DT Article DE Dermestes maculatus; Hide beetle; Stored products; Rearing; Refugia; Cannibalism ID HIDE BEETLE; LESSER MEALWORM; POULTRY HOUSES; COLEOPTERA; COMBINATION; EFFICACY; PESTS AB The hide beetle, Dermestes maculatus DeGeer, is a Dermestid beetle that can infest a wide variety of stored products, including pet foods, animal feeds, dried foods, and grain products with high protein content. Although there is published information concerning the biology and habits of D. maculatus, there are few studies that examine these factors in terms of mass rearing for maintenance of laboratory cultures. Multiple experiments with factors such as diet type and amount, container size, refugia type, and amount and effect of larval density were examined to assess methodologies that could be utilized in mass rearing. Protein-rich diet sources such as commercial pet food, nutrition drink mix, and bone meal provided adequate nutrition for D. maculatus and supported development from egg to adult. Cannibalism by larvae and adults, especially on the pupae, was common but could be minimized by providing refugia for larvae to utilize as pupation sites. At greater larval densities, cork and wood refugia increased survival to the adult stage by nearly 50 % by reducing cannibalization. Results show that D. maculatus can be mass reared successfully in the laboratory, but the diet must have adequate protein content, and precautions must be taken to reduce cannibalism. C1 [Fontenot, Emily A.] FAO IAEA Agr & Biotechnol Lab, A-2444 Seibersdorf, Austria. [Fontenot, Emily A.; Arthur, Frank H.; Hartzer, Kris L.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. RP Fontenot, EA (reprint author), FAO IAEA Agr & Biotechnol Lab, Reaktorstr 1, A-2444 Seibersdorf, Austria. EM eagnesfontenot@gmail.com NR 17 TC 0 Z9 0 U1 4 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD MAR PY 2015 VL 88 IS 1 BP 113 EP 119 DI 10.1007/s10340-014-0562-x PG 7 WC Entomology SC Entomology GA CC0PW UT WOS:000350039000013 ER PT J AU Thomas, PR Karriker, LA Ramirez, A Zhang, JQ Ellingson, JS Crawford, KK Bates, JL Hammen, KJ Holtkamp, DJ AF Thomas, Paul R. Karriker, Locke A. Ramirez, Alejandro Zhang, Jianqiang Ellingson, Josh S. Crawford, Kimberly K. Bates, Jessica L. Hammen, Kristin J. Holtkamp, Derald J. TI Evaluation of time and temperature sufficient to inactivate porcine epidemic diarrhea virus in swine feces on metal surfaces SO JOURNAL OF SWINE HEALTH AND PRODUCTION LA English DT Article DE swine; porcine epidemic diarrhea virus; inactivation; temperature; thermo-assisted drying and decontamination ID RESPIRATORY SYNDROME VIRUS; UNITED-STATES; TRANSPORT VEHICLES; CORONAVIRUS AB Objectives: To determine temperature and time applications sufficient to inactivate porcine epidemic diarrhea virus (PEDV) on a commercial livestock trailer, and practical within the constraints of current thermo-assisted drying and decontamination (TADD) capabilities in the industry. Materials and methods: Thirty-two 4-week-old barrows were inoculated via oral gastric tube with 5 mL of either PEDV-negative feces (Neg; n = 4), untreated PEDV-positive feces (Pos; n = 4), or PEDV-positive feces subjected to 71 degrees C for 10 minutes (71C-10M; n = 4), 63 degrees C for 10 minutes (63C-10M; n = 4), 54 degrees C for 10 minutes (54C-10M; n = 4), 38 degrees C for 12 hours (38C-12H; n = 4), 20 degrees C for 24 hours (20C-24H; n = 4), or 20 degrees C for 7 days (20C-7D; n = 4). These pigs served as a bioassay to determine the infectivity of virus following treatment. Bioassay results were determined by reverse-transcriptase polymerase chain reaction on rectal swabs collected from the inoculated pigs on days 3 and 7 post inoculation. Results: None Of the pigs in the 71C-10M and 20C-7D groups became infected with PEDV. This result differed significantly from that of the Pos group (P < .05). Results of the other groups did not differ significantly from that of the Pos group (P>.05). Implication: Holding PEDV in the presence of feces at 71 degrees C for 10 minutes or at 20 degrees C (room temperature) for 7 days is sufficient to inactivate the virus, preventing transmission under the conditions of this study. C1 [Thomas, Paul R.; Karriker, Locke A.; Ellingson, Josh S.; Bates, Jessica L.; Hammen, Kristin J.] Iowa State Univ, Coll Vet Med, Swine Med Educ Ctr, Ames, IA USA. [Karriker, Locke A.; Ramirez, Alejandro; Zhang, Jianqiang; Holtkamp, Derald J.] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Thomas, Paul R.; Ellingson, Josh S.] AMVC Management Serv, Audubon, IA 50025 USA. [Crawford, Kimberly K.] ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Thomas, PR (reprint author), AMVC Management Serv, 508 Market St, Audubon, IA 50025 USA. EM pthomas@amvcms.com FU National Pork Board FX Funding was provided by The National Pork Board. The authors would also like to thank AMVC Management Services for in-kind support and students and staff of the Swine Medicine Education Center at Iowa State for assistance with live-animal work. NR 18 TC 2 Z9 2 U1 1 U2 6 PU AMER ASSOC SWINE VETERINARIANS PI PERRY PA 902 1ST AVE, PERRY, IA 50220-1703 USA SN 1537-209X J9 J SWINE HEALTH PROD JI J. Swine. Health Prod. PD MAR-APR PY 2015 VL 23 IS 2 BP 84 EP 90 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA CC0ES UT WOS:000350009200006 ER PT J AU Mun, SP Cai, ZY Zhang, JL AF Mun, Sung Phil Cai, Zhiyong Zhang, Jilei TI Preparation of Fe-cored carbon nanomaterials from mountain pine beetle-killed pine wood SO MATERIALS LETTERS LA English DT Article DE Mountain pine beetle; Pine wood; Fe-cored carbon nanomaterials (Fe-CNs); Carbon shell; Iron carbides; alpha-Fe; gamma-Fe ID IRON NANOPARTICLES; CARBIDE; CHAR AB The mountain pine beetle-killed lodgepole pine (Pinus contorta) wood treated with iron (III) nitrate solution was used for the preparation of Fe-cored carbon nanomaterials (Fe-CNs) under various carbonization temperatures. The carbonization yield of Fe-treated sample (5% as Fe) was always 1-3% higher (after ash compensation) than that of the non-treated samples heated at the same condition. The lowest carbonization temperature required to produce Fe-CNs was 700 degrees C. The carbon shell was composed of 30-40 well-aligned layers of graphitic carbon nanostructure. The iron captured by graphitic layers was assumed to be iron carbides and/or alpha-Fe and gamma-Fe. This study indicates that at least 700 degrees C of carbonization temperature is needed for the production of Fe-CNs and the mountain pine beetle-killed pine wood can be a carbon source for the production of Fe-CNs. (C) 2014 Elsevier B.V. All rights reserved. C1 [Mun, Sung Phil] Chonbuk Natl Univ, Dept Wood Sci & Technol, Jeonju 561756, Jeonbuk, South Korea. [Cai, Zhiyong] US Forest Serv, USDA, Dept Forest Prod, Madison, WI 53726 USA. [Zhang, Jilei] Mississippi State Univ, Dept Forest Prod, Mississippi State, MS 39762 USA. RP Zhang, JL (reprint author), Mississippi State Univ, Dept Forest Prod, Mississippi State, MS 39762 USA. EM jzhang@cfr.msstate.edu FU USDA Forest Service [11-JV-11111124-129] FX This work was supported by the USDA Forest Service through Grant no. 11-JV-11111124-129. NR 12 TC 0 Z9 0 U1 3 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-577X EI 1873-4979 J9 MATER LETT JI Mater. Lett. PD MAR 1 PY 2015 VL 142 BP 45 EP 48 DI 10.1016/j.matlet.2014.11.053 PG 4 WC Materials Science, Multidisciplinary; Physics, Applied SC Materials Science; Physics GA CB6HQ UT WOS:000349728500013 ER PT J AU Ballester, AR Marcet-Houben, M Levin, E Sela, N Selma-Lazaro, C Carmona, L Wisniewski, M Droby, S Gonzalez-Candelas, L Gabaldon, T AF Ballester, Ana-Rosa Marcet-Houben, Marina Levin, Elena Sela, Noa Selma-Lazaro, Cristina Carmona, Lourdes Wisniewski, Michael Droby, Samir Gonzalez-Candelas, Luis Gabaldon, Toni TI Genome, Transcriptome, and Functional Analyses of Penicillium expansum Provide New Insights Into Secondary Metabolism and Pathogenicity SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID MULTIPLE SEQUENCE ALIGNMENT; CARBOHYDRATE-ACTIVE ENZYMES; GLUCONIC ACID; TERVERTICILLATE PENICILLIA; PATULIN BIOSYNTHESIS; ENDOPHYTIC FUNGUS; ANALYSIS TOOLKIT; BLUE MOLD; GENE; APPLE AB The relationship between secondary metabolism and infection in pathogenic fungi has remained largely elusive. The genus Penicillium comprises a group of plant pathogens with varying host specificities and with the ability to produce a wide array of secondary metabolites. The genomes of three Penicillium expansum strains, the main post-harvest pathogen of pome fruit, and one Pencillium italicum strain, a post-harvest pathogen of citrus fruit, were sequenced and compared with 24 other fungal species. A genomic analysis of gene clusters responsible for the production of secondary metabolites was performed. Putative virulence factors in P. expansum were identified by means of a transcriptomic analysis of apple fruits during the course of infection. Despite a major genome contraction, P. expansum is the Penicillium species with the largest potential for the production of secondary metabolites. Results using knockout mutants clearly demonstrated that neither patulin nor citrinin are required by P. expansum to successfully infect apples. C1 [Ballester, Ana-Rosa; Selma-Lazaro, Cristina; Carmona, Lourdes; Gonzalez-Candelas, Luis] CSIC, Inst Agroquim & Tecnol Alimentos, Valencia 46980, Spain. [Marcet-Houben, Marina; Gabaldon, Toni] CRG, Bioinformat & Genom Programme, Barcelona 08003, Spain. [Marcet-Houben, Marina; Gabaldon, Toni] Univ Pompeu Fabra, Barcelona 08003, Spain. [Levin, Elena; Sela, Noa; Droby, Samir] ARO, Volcani Ctr, Dept Postharvest Sci, IL-50250 Bet Dagan, Israel. [Wisniewski, Michael] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Gabaldon, Toni] ICREA, Barcelona 08010, Spain. RP Gabaldon, T (reprint author), CRG, Bioinformat & Genom Programme, Dr Aiguader 88, Barcelona 08003, Spain. EM lgonzalez@iata.csic.es; tgabaldon@crg.es RI Sela, Noa/C-5133-2008; Marcet-Houben, Marina/H-2170-2015; Ballester, Ana Rosa/D-9677-2011; OI Marcet-Houben, Marina/0000-0003-4838-187X; Ballester, Ana Rosa/0000-0002-7688-7838; Gabaldon, Toni/0000-0003-0019-1735; Gonzalez-Candelas, Luis/0000-0002-2579-6880 FU Spanish Ministry of Economy and Innovation [AGL-2008-04828-C03-02, AGL2011-30519-C03-01]; Generalitat Valenciana, Spain [PROMETEO/2010/010, PROMETEOII/2014/027]; CSIC; European Social Fund; FPI Predoctoral fellowship from the Spanish MICINN [BES-2012-054595]; Spanish ministry of Economy and Competitiveness [BIO2012-37161]; Qatar National Research Fund grant [NPRP 5-298-3-086]; European Research Council under the European Union [ERC-2012-StG-310325] FX The technical assistance of A. Izquierdo (IATA-CSIC, Valencia, Spain) is gratefully acknowledged. We are grateful to H. Himmelbauer and the CRG sequencing core facility for their technical assistance. Work at L. Gonzalez-Candelas' lab was partially supported by the Spanish Ministry of Economy and Innovation (Research Grants AGL-2008-04828-C03-02 and AGL2011-30519-C03-01) and by the Generalitat Valenciana, Spain (PROMETEO/2010/010 and PROMETEOII/2014/027). A.-R. Ballester is grateful to CSIC and the European Social Fund for her postdoctoral contract JAE-Doc. C. Selma-Lazaro is recipient of a FPI Predoctoral fellowship from the Spanish MICINN (BES-2012-054595). The T. Gabaldon group research is funded, in part, by a grant from the Spanish ministry of Economy and Competitiveness (BIO2012-37161), a Grant from the Qatar National Research Fund grant (NPRP 5-298-3-086), and a grant from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC (Grant Agreement n. ERC-2012-StG-310325). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 88 TC 29 Z9 29 U1 9 U2 74 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD MAR PY 2015 VL 28 IS 3 BP 232 EP 248 DI 10.1094/MPMI-09-14-0261-FI PG 17 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA CB7BL UT WOS:000349781000004 PM 25338147 ER PT J AU Brown, DW Lee, SH Kim, LH Ryu, JG Lee, S Seo, Y Kim, YH Busman, M Yun, SH Proctor, RH Lee, T AF Brown, Daren W. Lee, Seung-Ho Kim, Lee-Han Ryu, Jae-Gee Lee, Soohyung Seo, Yunhee Kim, Young Ho Busman, Mark Yun, Sung-Hwan Proctor, Robert H. Lee, Theresa TI Identification of a 12-Gene Fusaric Acid Biosynthetic Gene Cluster in Fusarium Species Through Comparative and Functional Genomics SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID IN-VITRO; VERTICILLIOIDES; TRANSFORMATION; PATHOGENICITY; NORMALIZATION; GRAMINEARUM; SUSPENSION; ENDOPHYTE; CELLS AB In fungi, genes involved in biosynthesis of a secondary metabolite (SM) are often located adjacent to one another in the genome and are coordinately regulated. These SM biosynthetic gene clusters typically encode enzymes, one or more transcription factors, and a transport protein. Fusaric acid is a polyketide-derived SM produced by multiple species of the fungal genus Fusarium. This SM is of concern because it is toxic to animals and, therefore, is considered a mycotoxin and may contribute to plant pathogenesis. Preliminary descriptions of the fusaric acid (FA) biosynthetic gene (FUB) cluster have been reported in two Fusarium species, the maize pathogen E verticillioides and the rice pathogen E fujikuroi. The cluster consisted of five genes and did not include a transcription factor or transporter gene. Here, analysis of the FUB region in E verticillioides, E fujikuroi, and E oxysporum, a plant pathogen with multiple hosts, indicates the FUB cluster consists of at least 12 genes (FUB1 to FUB12). Deletion analysis confirmed that nine FUB genes, including two Zn(II)(2)Cys(6) transcription factor genes, are required for production of wild-type levels of FA. Comparisons of FUB cluster homologs across multiple Fusarium isolates and species revealed insertion of non-FUB genes at one or two locations in some homologs. Although the ability to produce FA contributed to the phytotoxicity of E oxysporum culture extracts, lack of production did not affect virulence of E oxysporum on cactus or E verticillioides on maize seedlings. These findings provide new insights into the genetic and biochemical processes required for FA production. C1 [Brown, Daren W.; Busman, Mark; Proctor, Robert H.] USDA ARS NCAUR, Peoria, IL 61604 USA. [Lee, Seung-Ho] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Ginseng Res Div, Eumseong 369873, South Korea. [Kim, Lee-Han; Ryu, Jae-Gee; Lee, Soohyung; Lee, Theresa] Rural Dev Adm, Natl Acad Agr Sci, Microbial Safety Team, Wonju 565851, South Korea. [Seo, Yunhee; Kim, Young Ho] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151921, South Korea. [Seo, Yunhee; Kim, Young Ho] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea. [Yun, Sung-Hwan] Soonchunhyang Univ, Dept Med Biotechnol, Asan 336745, South Korea. RP Brown, DW (reprint author), USDA ARS NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM daren.brown@ars.usda.gov; tessyll@korea.kr FU Research Program for Agricultural Science & Technology Development, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ008432, PJ008635]; Next-Generation Bio Green21 Program, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ008210] FX We thank C. McGovern, C. Probyn, and M. Moore for technical assistance and D. Palmquist for assistance with the statistical analysis. Part of the study was supported by the "Research Program for Agricultural Science & Technology Development (Project No. PJ008432 and PJ008635)" and a grant from the "Next-Generation Bio Green21 Program (No. PJ008210)", National Academy of Agricultural Science, Rural Development Administration, Republic of Korea. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 43 TC 8 Z9 8 U1 9 U2 52 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD MAR PY 2015 VL 28 IS 3 BP 319 EP 332 DI 10.1094/MPMI-09-14-0264-R PG 14 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA CB7BL UT WOS:000349781000011 PM 25372119 ER PT J AU Seidl, MF Faino, L Shi-Kunne, X van den Berg, GCM Bolton, MD Thomma, BPHJ AF Seidl, Michael F. Faino, Luigi Shi-Kunne, Xiaoqian van den Berg, Grardy C. M. Bolton, Melvin D. Thomma, Bart P. H. J. TI The Genome of the Saprophytic Fungus Verticillium tricorpus Reveals a Complex Effector Repertoire Resembling That of Its Pathogenic Relatives SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID CHITIN-TRIGGERED IMMUNITY; HIDDEN MARKOV MODEL; RNA-SEQ; EUKARYOTIC GENOMES; SIGNAL PEPTIDES; PLANT-PATHOGENS; PROTEIN FAMILY; EVOLUTION; ANNOTATION; WILT AB Vascular wilts caused by Verticillium spp. are destructive plant diseases affecting hundreds of hosts. Only a few Verticillium spp. are causal agents of vascular wilt diseases, of which V dahliae is the most notorious pathogen, and several V dahliae genomes are available. In contrast, V tricorpus is mainly known as a saprophyte and causal agent of opportunistic infections. Based on a hybrid approach that combines second and third generation sequencing, a near-gapless V tricorpus genome assembly was obtained. With comparative genomics, we sought to identify genomic features in V dahliae that confer the ability to cause vascular wilt disease. Unexpectedly, both species encode similar effector repertoires and share a genomic structure with genes encoding secreted proteins clustered in genomic islands. Intriguingly, V tricorpus contains significantly fewer repetitive elements and an extended spectrum of secreted carbohydrate-active enzymes when compared with V dahliae. In conclusion, we highlight the technical advances of a hybrid sequencing and assembly approach and show that the saprophyte V tricorpus shares many hallmark features with the pathogen V dahliae. C1 [Seidl, Michael F.; Faino, Luigi; Shi-Kunne, Xiaoqian; van den Berg, Grardy C. M.; Thomma, Bart P. H. J.] Wageningen Univ, Lab Phytopathol, NL-6708 PB Wageningen, Netherlands. [Bolton, Melvin D.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. RP Thomma, BPHJ (reprint author), Wageningen Univ, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. EM bart.thomma@wur.nl OI Seidl, Michael F/0000-0002-5218-2083; faino, luigi/0000-0002-6807-4191 FU Research Council Earth and Life Sciences (ALW) of the Netherlands Organization of Scientific Research (NWO) FX The authors acknowledge support by the Research Council Earth and Life Sciences (ALW) of the Netherlands Organization of Scientific Research (NWO). Moreover, we thank B. Essenstam for excellent plant care and X. Wang (USDA ARS) for excellent technical assistance. NR 79 TC 7 Z9 7 U1 3 U2 28 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD MAR PY 2015 VL 28 IS 3 BP 362 EP 373 DI 10.1094/MPMI-06-14-0173-R PG 12 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA CB7BL UT WOS:000349781000014 PM 25208342 ER PT J AU De Graff, JV Gallegos, AJ Reid, ME LaHusen, RG Denlinger, RP AF De Graff, Jerome V. Gallegos, Alan J. Reid, Mark E. LaHusen, Richard G. Denlinger, Roger P. TI Using monitoring and modeling to define the hazard posed by the reactivated Ferguson rock slide, Merced Canyon, California SO NATURAL HAZARDS LA English DT Article DE Landslide; Rock slide; Real-time monitoring; Simulation modeling; Emergency response; California ID IRREGULAR 3-DIMENSIONAL TERRAIN; GRANULAR AVALANCHES; GEOMORPHOLOGY AB Rapid onset natural disasters such as large landslides create a need for scientific information about the event, which is vital to ensuring public safety, restoring infrastructure, preventing additional damage, and resuming normal economic activity. At the same time, there is limited data available upon which to base reliable scientific responses. Monitoring movement and modeling runout are mechanisms for gaining vital data and reducing the uncertainty created about a rapid onset natural disaster. We examine the effectiveness of this approach during the 2006 Ferguson rock slide disaster, which severed California Highway 140. Even after construction of a bypass restoring normal access to the community of El Portal, CA and a major entrance to Yosemite National Park, significant scientific questions remained. The most important for the affected public and emergency service agencies was the likelihood that access would again be severed during the impending rainy season and the possibility of a landslide dam blocking flow in the Merced River. Real-time monitoring of the Ferguson rock slide yielded clear information on the continuing movement of the rock slide and its implications for emergency response actions. Similarly, simulation of runout deposits using a physically based model together with volumes and slope steepness information demonstrated the conditions necessary for a landslide dam-forming event and the possible consequences of such an event given the dimensions of potential rock slide deposits. C1 [De Graff, Jerome V.; Gallegos, Alan J.] US Forest Serv, USDA, Clovis, CA 93611 USA. [Reid, Mark E.] US Geol Survey, Menlo Pk, CA 94025 USA. [LaHusen, Richard G.; Denlinger, Roger P.] US Geol Survey, Vancouver, WA USA. RP De Graff, JV (reprint author), US Forest Serv, USDA, Clovis, CA 93611 USA. EM jdegraff@csufresno.edu NR 24 TC 1 Z9 1 U1 0 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0921-030X EI 1573-0840 J9 NAT HAZARDS JI Nat. Hazards PD MAR PY 2015 VL 76 IS 2 BP 769 EP 789 DI 10.1007/s11069-014-1518-4 PG 21 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Water Resources SC Geology; Meteorology & Atmospheric Sciences; Water Resources GA CC4SL UT WOS:000350343600003 ER PT J AU Wong, GK Shulman, RJ Chumpitazi, BP AF Wong, G. K. Shulman, R. J. Chumpitazi, B. P. TI Gastric emptying scintigraphy results in children are affected by age, anthropometric factors, and study duration SO NEUROGASTROENTEROLOGY AND MOTILITY LA English DT Article DE children; dyspepsia; gastric emptying; gastroparesis; motility; nuclear medicine ID CLINICAL CHARACTERISTICS; INTERSTITIAL-CELLS; BREATH TEST; GASTROPARESIS; MOTILITY; ACCOMMODATION; SYMPTOMS; OUTCOMES; CAJAL AB BackgroundA standardized 4-h adult-based gastric emptying scintigraphy (GES) protocol is increasingly being used in children to evaluate for gastroparesis. We sought to determine the effect of age, anthropometrics, and study duration on GES results using this protocol in children. MethodsRetrospective review of children who underwent a 4-h solid-meal GES study at a tertiary care center. GES results and anthropometric data (e.g., weight, stature, body surface area [BSA]) were systematically captured. Key ResultsOf 216 children, 188 (87%) were able to complete the study meal. Children unable to complete the meal were younger and smaller. In multivariate analysis, only increasing BSA was identified as being positively associated with ability to complete the meal (odds ratio: 19.7; p<0.001). Of those completing the meal, 48 (26%) had delayed emptying (4-h retention value >10%). These children were significantly younger and smaller than those with normal emptying. In multivariate analysis of those completing the meal, only increasing BSA (odds ratio: 0.26; p=0.006) was identified as being negatively associated with delayed emptying. There was a progressive increase in the positive predictive value for identification of delayed gastric emptying as the duration of the study increased (0.25, 0.60, and 0.71 at 1, 2, and 3h, respectively) using the 4-hr value as a comparator. Conclusions & InferencesYoung children have more difficulty completing the GES meal. Childhood gastric retention is affected by age and anthropometric factors, primarily BSA. The standardized 4-h GES protocol may need to take these factors into account in children. C1 [Wong, G. K.; Shulman, R. J.; Chumpitazi, B. P.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Wong, G. K.; Shulman, R. J.; Chumpitazi, B. P.] Texas Childrens Hosp, Houston, TX 77030 USA. [Shulman, R. J.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Chumpitazi, BP (reprint author), Texas Childrens Hosp, 6701 Fannin St,CCC 1010-03, Houston, TX 77030 USA. EM chumpita@bcm.edu FU National Institutes of Health [R01 NR05337, NR013497]; Daffy's Foundation; USDA/ARS [6250-51000-043, P30 DK56338]; NASPGHAN Foundation/Nestle Nutrition Young Investigator Development Award; [K23 DK101688] FX This study was supported in part by R01 NR05337 and NR013497 from the National Institutes of Health, the Daffy's Foundation, the USDA/ARS under Cooperative Agreement No. 6250-51000-043, and P30 DK56338 which funds the Texas Medical Center Digestive Disease Center. Funding was also provided by the NASPGHAN Foundation/Nestle Nutrition Young Investigator Development Award and K23 DK101688 (B.P.C.). NR 25 TC 1 Z9 1 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1350-1925 EI 1365-2982 J9 NEUROGASTROENT MOTIL JI Neurogastroenterol. Motil. PD MAR PY 2015 VL 27 IS 3 BP 356 EP 362 DI 10.1111/nmo.12499 PG 7 WC Gastroenterology & Hepatology; Clinical Neurology; Neurosciences SC Gastroenterology & Hepatology; Neurosciences & Neurology GA CC2CV UT WOS:000350153400007 PM 25557417 ER PT J AU Hajek, AE Tobin, PC Haynes, KJ AF Hajek, Ann E. Tobin, Patrick C. Haynes, Kyle J. TI Replacement of a dominant viral pathogen by a fungal pathogen does not alter the collapse of a regional forest insect outbreak SO OECOLOGIA LA English DT Article DE Epizootiology; Environmental drivers; Insect outbreaks; Spatial lag model; Dependence ID GYPSY-MOTH LEPIDOPTERA; NUCLEAR POLYHEDROSIS-VIRUS; SPATIAL DENSITY-DEPENDENCE; ENTOMOPHAGA-MAIMAIGA; LYMANTRIA-DISPAR; POPULATION-DYNAMICS; INFECTIOUS-DISEASES; NORTH-AMERICA; LONG-TERM; ENTOMOPHTHORALES AB Natural enemies and environmental factors likely both influence the population cycles of many forest-defoliating insect species. Previous work suggests precipitation influences the spatiotemporal patterns of gypsy moth outbreaks in North America, and it has been hypothesized that precipitation could act indirectly through effects on pathogens. We investigated the potential role of climatic and environmental factors in driving pathogen epizootics and parasitism at 57 sites over an area of a parts per thousand 72,300 km(2) in four US mid-Atlantic states during the final year (2009) of a gypsy moth outbreak. Prior work has largely reported that the Lymantria dispar nucleopolyhedrovirus (LdNPV) was the principal mortality agent responsible for regional collapses of gypsy moth outbreaks. However, in the gypsy moth outbreak-prone US mid-Atlantic region, the fungal pathogen Entomophaga maimaiga has replaced the virus as the dominant source of mortality in dense host populations. The severity of the gypsy moth population crash, measured as the decline in egg mass densities from 2009 to 2010, tended to increase with the prevalence of E. maimaiga and larval parasitoids, but not LdNPV. A significantly negative spatial association was detected between rates of fungal mortality and parasitism, potentially indicating displacement of parasitoids by E. maimaiga. Fungal, viral, and parasitoid mortality agents differed in their associations with local abiotic and biotic conditions, but precipitation significantly influenced both fungal and viral prevalence. This study provides the first spatially robust evidence of the dominance of E. maimaiga during the collapse of a gypsy moth outbreak and highlights the important role played by microclimatic conditions. C1 [Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Tobin, Patrick C.] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. [Haynes, Kyle J.] Univ Virginia, Dept Environm Sci, Blandy Expt Farm, Boyce, VA 22620 USA. RP Hajek, AE (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM aeh4@cornell.edu FU USDA Forest Service, Forest Health Protection USDA Forest Service grant [07-CA-11420004-152] FX We thank R. Rabaglia and R. Reardon (USDA Forest Service) for helping to conceive, assist, and design this study. This study was only possible due to extensive assistance from R. Tatman (MD Department of Agriculture), T. Marasco (PA Bureau of Forestry), C. Asaro (VA Department of Forestry), and R. Turcotte (USDA Forest Service, Morgantown, WV) and their teams. At Cornell, samples were diagnosed and data were collated by R. Plymale, S. Long, K. Ciccaglione, C. Fritzen, M. Garvey, M. Grambor, T. James, A. Navarro, and J. Tyvoll, and J. Nix counted resting spore densities in soil samples. We thank L. Blackburn (USDA Forest Service) and J. Walter (University of Virginia) for technical assistance, and A. Liebhold and two anonymous reviewers for comments on the manuscript. This study was funded by USDA Forest Service, Forest Health Protection USDA Forest Service grant #07-CA-11420004-152. NR 78 TC 4 Z9 4 U1 4 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD MAR PY 2015 VL 177 IS 3 BP 785 EP 797 DI 10.1007/s00442-014-3164-7 PG 13 WC Ecology SC Environmental Sciences & Ecology GA CC0NV UT WOS:000350033500015 PM 25510217 ER PT J AU Woodall, CW Russell, MB Walters, BF D'Amato, AW Fraver, S Domke, GM AF Woodall, C. W. Russell, M. B. Walters, B. F. D'Amato, A. W. Fraver, S. Domke, G. M. TI Net carbon flux of dead wood in forests of the Eastern US SO OECOLOGIA LA English DT Article DE Downed dead wood; Detritus; Stand dynamics; Random forests model; Decay classes ID UNITED-STATES; BOREAL FORESTS; DEBRIS; MANAGEMENT; CLIMATE; DISTURBANCE; ECOLOGY; BIOMASS; DECOMPOSITION; INVENTORY AB Downed dead wood (DDW) in forest ecosystems is a C pool whose net flux is governed by a complex of natural and anthropogenic processes and is critical to the management of the entire forest C pool. As empirical examination of DDW C net flux has rarely been conducted across large scales, the goal of this study was to use a remeasured inventory of DDW C and ancillary forest attributes to assess C net flux across forests of the Eastern US. Stocks associated with large fine woody debris (diameter 2.6-7.6 cm) decreased over time (-0.11 Mg ha(-1) year(-1)), while stocks of larger-sized coarse DDW increased (0.02 Mg ha(-1) year(-1)). Stocks of total DDW C decreased (-0.14 Mg ha(-1) year(-1)), while standing dead and live tree stocks both increased, 0.01 and 0.44 Mg ha(-1) year(-1), respectively. The spatial distribution of DDW C stock change was highly heterogeneous with random forests model results indicating that management history, live tree stocking, natural disturbance, and growing degree days only partially explain stock change. Natural disturbances drove substantial C transfers from the live tree pool (a parts per thousand a'4 Mg ha(-1) year(-1)) to the standing dead tree pool (a parts per thousand 3 Mg ha(-1) year(-1)) with only a minimal increase in DDW C stocks (a parts per thousand 1 Mg ha(-1) year(-1)) in lower decay classes, suggesting a delayed transfer of C to the DDW pool. The assessment and management of DDW C flux is complicated by the diversity of natural and anthropogenic forces that drive their dynamics with the scale and timing of flux among forest C pools remaining a large knowledge gap. C1 [Woodall, C. W.; Walters, B. F.; Domke, G. M.] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. [Russell, M. B.; D'Amato, A. W.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Fraver, S.] Univ Maine, Sch Forest Resources, Orono, ME USA. RP Woodall, CW (reprint author), US Forest Serv, No Res Stn, USDA, 1992 Folwell Ave, St Paul, MN 55108 USA. EM cwoodall@fs.fed.us OI Domke, Grant/0000-0003-0485-0355 NR 64 TC 4 Z9 4 U1 5 U2 34 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD MAR PY 2015 VL 177 IS 3 BP 861 EP 874 DI 10.1007/s00442-014-3171-8 PG 14 WC Ecology SC Environmental Sciences & Ecology GA CC0NV UT WOS:000350033500021 PM 25430045 ER PT J AU Shea, MK Kritchevsky, SB Hsu, FC Nevitt, M Booth, SL Kwoh, CK McAlindon, TE Vermeer, C Drummen, N Harris, TB Womack, C Loeser, RF AF Shea, M. K. Kritchevsky, S. B. Hsu, F. -C. Nevitt, M. Booth, S. L. Kwoh, C. K. McAlindon, T. E. Vermeer, C. Drummen, N. Harris, T. B. Womack, C. Loeser, R. F. CA Hlth ABC Study TI The association between vitamin K status and knee osteoarthritis features in older adults: The Health, Aging and Body Composition Study SO OSTEOARTHRITIS AND CARTILAGE LA English DT Article DE Osteoarthritis; Nutrition; Phylloquinone; Vitamin K; Matrix gla protein; Epidemiology ID MATRIX GLA PROTEIN; BONE MORPHOGENETIC PROTEIN-2; COMMUNITY-DWELLING ADULTS; ARTICULAR-CARTILAGE; ELDERLY PERSONS; MENISCAL TEAR; CALCIUM; SYMPTOMS; CALCIFICATION; DEPOSITION AB Background: Vitamin K-dependent (VKD) proteins, including the mineralization inhibitor matrix-gla protein (MGP), are found in joint tissues including cartilage and bone. Previous studies suggest low vitamin K status is associated with higher osteoarthritis (OA) prevalence and incidence. Objective: To clarify what joint tissues vitamin K is relevant to in OA, we investigated the cross-sectional and longitudinal association between vitamin K status and knee OA structural features measured using magnetic resonance imaging (MRI). Methods: Plasma phylloquinone (PK, vitamin K1) and dephosphorylated-uncarboxylated MGP ((dp) ucMGP) were measured in 791 older community-dwelling adults who had bilateral knee MRIs (mean +/- SD age = 74 +/- 3 y; 67% female). The adjusted odds ratios (and 95% confidence intervals) [OR (95%CI)] for presence and progression of knee OA features according to vitamin K status were calculated using marginal models with generalized estimating equations (GEEs), adjusted for age, sex, body mass index (BMI), triglycerides and other pertinent confounders. Results: Longitudinally, participants with very low plasma PK (<0.2 nM) were more likely to have articular cartilage and meniscus damage progression after 3 years [OR (95% CIs): 1.7(1.0-3.0), 2.6(1.3-5.2) respectively] compared to sufficient PK (>= 1.0 nM). Higher plasma (dp) ucMGP (reflective of lower vitamin K status) was associated with higher odds of meniscus damage, osteophytes, bone marrow lesions, and subarticular cysts cross-sectionally [ORs (95% CIs) comparing highest to lowest quartile: 1.6(1.1-2.3); 1.7(1.1-2.5); 1.9(1.3-2.8); 1.5(1.0-2.1), respectively]. Conclusion: Community-dwelling men and women with very low plasma PK were more likely to have progression of articular cartilage and meniscus damage. Plasma (dp) ucMGP was associated with presence of knee OA features but not progression. Future studies are needed to clarify mechanisms underlying vitamin Ks role in OA. (C) 2014 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. C1 [Shea, M. K.; Booth, S. L.] Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Kritchevsky, S. B.] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. [Hsu, F. -C.] Wake Forest Sch Med, Div Publ Hlth Sci, Dept Biostat Sci, Winston Salem, NC USA. [Nevitt, M.] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. [Kwoh, C. K.] Univ Arizona, Div Rheumatol, Tucson, AZ USA. [McAlindon, T. E.] Tufts Med Ctr, Div Rheumatol, Boston, MA USA. [Vermeer, C.; Drummen, N.] VitaK, Maastricht, Netherlands. [Harris, T. B.] NIA, Lab Epidemiol & Populat Sci, Bethesda, MD 20892 USA. [Womack, C.] Univ Tennessee, Dept Prevent Med, Hlth Sci Ctr, Memphis, TN USA. [Loeser, R. F.] Univ N Carolina, Div Rheumatol Allergy & Immunol, Chapel Hill, NC USA. RP Shea, MK (reprint author), Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM kyla.shea@tufts.edu OI Vermeer, Cees/0000-0002-2300-8561 FU Intramural Research Program of the National Institutes of Health, National Institute on Aging [N01-AG-6-2101, N01-AG-6-2103, N01-AG-6-2106]; National Institute of Arthritis Musculoskeletal and Skin Diseases [R21AR062284, K01AR063167]; Arthritis Foundation; U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX This study was supported in part by the Intramural Research Program of the National Institutes of Health, National Institute on Aging, and contracts N01-AG-6-2101, N01-AG-6-2103, and N01-AG-6-2106; the National Institute of Arthritis Musculoskeletal and Skin Diseases (R21AR062284 and K01AR063167), a New Investigator Grant from the Arthritis Foundation and the U.S. Department of Agriculture, Agricultural Research Service under Cooperative Agreement No. 58-1950-7-707. NR 42 TC 8 Z9 8 U1 1 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1063-4584 EI 1522-9653 J9 OSTEOARTHR CARTILAGE JI Osteoarthritis Cartilage PD MAR PY 2015 VL 23 IS 3 BP 370 EP 378 DI 10.1016/j.joca.2014.12.008 PG 9 WC Orthopedics; Rheumatology SC Orthopedics; Rheumatology GA CB9PF UT WOS:000349963300005 PM 25528106 ER PT J AU Hashimoto, M Aoki, Y Saito, S Suzuki, S AF Hashimoto, Maki Aoki, Yoshinao Saito, Seiya Suzuki, Shunji TI Characterisation of heteroplasmic status at codon 143 of the Botrytis cinerea cytochrome b gene in a semi-quantitative AS-PCR assay SO PEST MANAGEMENT SCIENCE LA English DT Article DE additional intron; AS-PCR; Botrytis cinerea; fungicide resistance; heteroplasmy; QoI fungicide ID PLASMOPARA-VITICOLA POPULATIONS; QO INHIBITOR FUNGICIDES; RESISTANCE; STROBILURIN; PATHOGENS AB BACKGROUNDAn in-depth understanding of quinone outside inhibitor (QoI)-fungicide-resistant Botrytis cinerea isolates in a vineyard is expected to contribute to the development of an optimum disease management programme for the control of grape grey mould. RESULTSThe resistance and structure of the cytochrome b gene in B. cinerea collected from a Japanese vineyard were characterised. The semi-quantitative allele-specific primer-polymerase chain reaction (AS-PCR) assay developed in the present study was able to distinguish heteroplasmic status from homoplasmic status at codon 143 of the cytochrome b gene in QoI-fungicide-resistant B. cinerea from vineyards in Japan. With this assay it was demonstrated that the repeated introduction of QoI fungicide selection pressure increased the ratio of G143A-mutated cytochrome b genes in B. cinerea isolates. CONCLUSIONIt is proposed that the semi-quantitative AS-PCR assay is a reliable tool for the detection of QoI fungicide resistance and the evaluation of homoplasmic/heteroplasmic status at codon 143 of the cytochrome b gene in B. cinerea isolates. (c) 2014 Society of Chemical Industry C1 [Hashimoto, Maki; Aoki, Yoshinao; Suzuki, Shunji] Univ Yamanashi, Inst Enol & Viticulture, Kofu, Yamanashi 4000005, Japan. [Saito, Seiya] USDA ARS, Commod Protect & Qual Res Unit, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. RP Suzuki, S (reprint author), Univ Yamanashi, Inst Enol & Viticulture, Kofu, Yamanashi 4000005, Japan. EM suzukis@yamanashi.ac.jp NR 17 TC 0 Z9 0 U1 4 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAR PY 2015 VL 71 IS 3 BP 467 EP 477 DI 10.1002/ps.3867 PG 11 WC Agronomy; Entomology SC Agriculture; Entomology GA CC0AV UT WOS:000349998100015 PM 25067839 ER PT J AU Bull, CT Gebben, SJ Goldman, PH Trent, M Hayes, RJ AF Bull, Carolee T. Gebben, Samantha J. Goldman, Polly H. Trent, Mark Hayes, Ryan J. TI Host Genotype and Hypersensitive Reaction Influence Population Levels of Xanthomonas campestris pv. vitians in Lettuce SO PHYTOPATHOLOGY LA English DT Article DE Lactuca sativa; X. hortorum ID BACTERIAL LEAF-SPOT; CULTIVAR; LEAVES; COLONIZATION; RESISTANCE; STRAINS; DISEASE AB Dynamics of population sizes of Xanthomonas campestris pv. vitians inoculated onto or into lettuce leaves were monitored on susceptible and resistant cultivars. In general, population growth was greater for susceptible (Clemente, Salinas 88, Vista Verde) than resistant (Batavia Reine des Glaces, Iceberg, Little Gem) cultivars. When spray-inoculated or infiltrated, population levels of X. campestris pv. vitians were consistently significantly lower on Little Gem than on susceptible cultivars, while differences in the other resistant cultivars were not consistently statistically significant. Populations increased at an intermediate rate on cultivars Iceberg and Batavia Reine des Glaces. There were significant positive correlations between bacterial concentration applied and disease severity for all cultivars, but bacterial titer had a significantly greater influence on disease severity in the susceptible cultivars than in Little Gem and an intermediate influence in Iceberg and Batavia Reine des Glaces. Infiltration of X. campestris pv. vitians strains into leaves of Little Gem resulted in an incompatible reaction, whereas compatible reactions were observed in all other cultivars. It appears that the differences in the relationship between population dynamics for Little Gem and the other cultivars tested were due to the hypersensitive response in cultivar Little Gem. These findings have implications for disease management and lettuce breeding because X. campestris pv. vitians interacts differently with cultivars that differ for resistance mechanisms. C1 [Bull, Carolee T.; Gebben, Samantha J.; Goldman, Polly H.; Trent, Mark; Hayes, Ryan J.] ARS, USDA, Crop Improvement & Protect Unit, Salinas, CA 93905 USA. [Gebben, Samantha J.] Hartnell Coll, Sci & Math Inst, Salinas, CA 93901 USA. RP Bull, CT (reprint author), ARS, USDA, Crop Improvement & Protect Unit, 1636 E Alisal St, Salinas, CA 93905 USA. EM Carolee.Bull@ars.usda.gov FU California Leafy Greens Research Program; California Department of Food and Agriculture; NSF Science Talent Expansion Project (STEP), Hartnell College, Salinas, CA [0525444] FX This research was supported by the California Leafy Greens Research Program and the California Department of Food and Agriculture. Partial funding for work by S. Gebben was provided by an NSF Science Talent Expansion Project (STEP) grant, No. 0525444, Hartnell College, Salinas, CA. Special thanks to Isaiah Martinez, Estefania Palacios, Andrew Roberts, Noemi Gomez, Ben Longcor, Ana Ibarra, Robert Lomeli, Bert Robinson, Jose Orozco, David Milligan, and Sharon Benzen for assistance with experiments. The mention of a trade name, proprietary product, or vendor does not constitute an endorsement, guarantee, or warranty by the U.S. Department of Agriculture and does not imply its approval or the exclusion of these or other products that may be suitable. NR 47 TC 3 Z9 3 U1 2 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 BP 316 EP 324 DI 10.1094/PHYTO-07-14-0185-R PG 9 WC Plant Sciences SC Plant Sciences GA CB8JA UT WOS:000349874100004 PM 25302523 ER PT J AU Childers, R Danies, G Myers, K Fei, ZJ Small, IM Fry, WE AF Childers, Richard Danies, Giovanna Myers, Kevin Fei, Zhangjun Small, Ian M. Fry, William E. TI Acquired Resistance to Mefenoxam in Sensitive Isolates of Phytophthora infestans SO PHYTOPATHOLOGY LA English DT Article ID EASTERN UNITED-STATES; MULTIDRUG-RESISTANCE; METALAXYL RESISTANCE; INSENSITIVITY; POPULATIONS; FUNGICIDES; MANNITOL; GENOTYPES; STRESS; ORIGIN AB The systemic fungicide mefenoxam has been important in the control of late blight disease caused by Phytophthora infestans. This phenylamide fungicide has a negative effect on the synthesis of ribosomal RNA; however, the genetic basis for inherited field resistance is still not completely clear. We recently observed that a sensitive isolate became tolerant after a single passage on mefenoxam-containing medium. Further analyses revealed that all sensitive isolates tested (in three diverse genotypes) acquired this resistance equally quickly. In contrast, isolates that were "resistant" to mefenoxam in the initial assessment (stably resistant) did not increase in resistance upon further exposure. However, there appeared to be a cost associated with acquired resistance in the initially sensitive isolates, in that isolates with acquired resistance grew more slowly on mefenoxam-free medium than did the same isolates that had never been exposed to mefenoxam. The acquired resistance of the sensitive isolates declined slightly with subsequent culturing on medium free of mefenoxam. To investigate the mechanism of acquired resistance, we employed strand-specific RNA sequencing. Many differentially expressed genes were genotype specific, but one set of genes was differentially expressed in all genotypes. Among these were several genes (a phospholipase "Pi-PLD-like-3,"two ATP-binding cassette superfamily [ABC] transporters, and a mannitol dehydrogenase) that were up-regulated and whose function might contribute to a resistance phenotype. C1 [Childers, Richard; Danies, Giovanna; Myers, Kevin; Small, Ian M.; Fry, William E.] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. RP Fry, WE (reprint author), Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA. EM wef1@cornell.edu FU Cornell University; Agriculture and Food Research Initiative Competitive Grants Program from the U.S. Department of Agriculture [2011-68004-30154] FX This work was supported by Cornell University and by the Agriculture and Food Research Initiative Competitive Grants Program Grant 2011-68004-30154 from the U.S. Department of Agriculture. We thank D. Camuzeaux for technical assistance. NR 34 TC 7 Z9 8 U1 3 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 BP 342 EP 349 DI 10.1094/PHYTO-05-14-0148-R PG 8 WC Plant Sciences SC Plant Sciences GA CB8JA UT WOS:000349874100007 PM 25226526 ER PT J AU Frenkel, O Cadle-Davidson, L Wilcox, WF Milgroom, MG AF Frenkel, Omer Cadle-Davidson, Lance Wilcox, Wayne F. Milgroom, Michael G. TI Mechanisms of Resistance to an Azole Fungicide in the Grapevine Powdery Mildew Fungus, Erysiphe necator SO PHYTOPATHOLOGY LA English DT Article ID UNCINULA-NECATOR; MYCOSPHAERELLA-GRAMINICOLA; INHIBITING FUNGICIDES; OBLIGATE BIOTROPH; GENETIC GROUPS; SENSITIVITY; GENOTYPES; PATHOGEN; MYCLOBUTANIL; POPULATIONS AB We studied the mechanisms of azole resistance in Erysiphe necator by quantifying the sensitivity to myclobutanil (EC50) in 65 isolates from the eastern United States and 12 from Chile. From each isolate, we sequenced the gene for sterol 14-demethylase (CYP51), and measured the expression of CYP51 and homologs of four putative efflux transporter genes, which we identified in the E. necator transcriptome. Sequence variation in CYP51 was relatively low, with sequences of 40 U.S. isolates identical to the reference sequence. Nine U.S. isolates and five from Chile carried a previously identified A to T nucleotide substitution in position 495 (A495T), which results in an amino acid substitution in codon 136 (Y136F) and correlates with high levels of azole resistance. We also found a nucleotide substitution in position 1119 (A1119C) in 15 U.S. isolates, whose mean EC50 value was equivalent to that for the Y136F isolates. Isolates carrying mutation A1119C had significantly greater CYP51 expression, even though A1119C does not affect the CYP51 amino acid sequence. Regression analysis showed no significant effects of the expression of efflux transporter genes on EC50. Both the Y136F mutation in CYP51 and increased CYP51 expression appear responsible for azole resistance in eastern U.S. populations of E. necator. C1 [Frenkel, Omer; Milgroom, Michael G.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Cadle-Davidson, Lance] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14456 USA. [Wilcox, Wayne F.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Sch Integrat Plant Sci, Geneva, NY 14456 USA. RP Milgroom, MG (reprint author), Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. EM mgm5@cornell.edu FU Vaadia-BARD Postdoctoral Fellowship from the United States-Israel Binational Agricultural Research and Development Fund (BARD) [FI-410-2008]; USDA-Viticulture Consortium-East award; Hatch project [NYC-153410] FX This research was supported by Vaadia-BARD Postdoctoral Fellowship Award No. FI-410-2008 from the United States-Israel Binational Agricultural Research and Development Fund (BARD) to O. Frenkel, a USDA-Viticulture Consortium-East award to L. Cadle-Davidson and W. F. Wilcox, and Hatch project NYC-153410 to M. G. Milgroom. We thank J. Burr for conducting myclobutanil sensitivity assays; M. Drott for statistical analyses; and F. Riveros of the Instituto de Investigaciones Agropecuarias (INIA) in La Serena, Chile, for supplying E. necator isolates from that region. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 46 TC 7 Z9 7 U1 5 U2 32 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 BP 370 EP 377 DI 10.1094/PHYTO-07-14-0202-R PG 8 WC Plant Sciences SC Plant Sciences GA CB8JA UT WOS:000349874100010 PM 25271353 ER PT J AU Webster, CG Frantz, G Reitz, SR Funderburk, JE Mellinger, HC McAvoy, E Turechek, WW Marshall, SH Tantiwanich, Y McGrath, MT Daughtrey, ML Adkins, S AF Webster, Craig G. Frantz, Galen Reitz, Stuart R. Funderburk, Joseph E. Mellinger, H. Charles McAvoy, Eugene Turechek, William W. Marshall, Spencer H. Tantiwanich, Yaowapa McGrath, Margaret T. Daughtrey, Margery L. Adkins, Scott TI Emergence of Groundnut ringspot virus and Tomato chlorotic spot virus in Vegetables in Florida and the Southeastern United States SO PHYTOPATHOLOGY LA English DT Article ID SCHULTZEI THYSANOPTERA THRIPIDAE; WILT-VIRUS; 1ST REPORT; FRANKLINIELLA-OCCIDENTALIS; PLANT-VIRUSES; SOUTH FLORIDA; TOSPOVIRUSES; CROPS; TRANSMISSION; ARGENTINA AB Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus (TCSV) are two emerging tospoviruses in Florida. In a survey of the southeastern United States, GRSV and TCSV were frequently detected in solanaceous crops and weeds with tospovirus-like symptoms in south Florida, and occurred sympatrically with Tomato spotted wilt virus (TSWV) in tomato and pepper in south Florida. TSWV was the only tospovirus detected in other survey locations, with the exceptions of GRSV from tomato (Solanrum lycopersicum) in South Carolina and New York, both of which are first reports. Impatiens (Impatiens walleriana) and lettuce (Lactuca sativa) were the only non-solanaceous GRSV and/or TCSV hosts identified in experimental host range studies. Little genetic diversity was observed in GRSV and TCSV sequences, likely due to the recent introductions of both viruses. All GRSV isolates characterized were reassortants with the TCSV M RNA. In laboratory transmission studies, Frankliniella schultzei was a more efficient vector of GRSV than F occidentalis. TCSV was acquired more efficiently than GRSV by F occidentalis but upon acquisition, transmission frequencies were similar. Further spread of GRSV and TCSV in the United States is possible and detection of mixed infections highlights the opportunity for additional reassortment of tospovirus genomic RNAs. C1 [Webster, Craig G.; Turechek, William W.; Marshall, Spencer H.; Adkins, Scott] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Frantz, Galen; Mellinger, H. Charles] Glades Crop Care Inc, Jupiter, FL 33458 USA. [Reitz, Stuart R.] Oregon State Univ, Dept Crop & Soil Sci, Ontario, OR 97914 USA. [Funderburk, Joseph E.] Univ Florida, Dept Entomol & Nematol, North Florida Res & Educ Ctr, Quincy, FL 32351 USA. [McAvoy, Eugene] Univ Florida, IFAS, Labelle, FL 33935 USA. [Tantiwanich, Yaowapa] Minist Agr & Cooperat, Plant Pathol Res Grp, Plant Protect Res & Dev Off, Dept Agr, Bangkok 10900, Thailand. [McGrath, Margaret T.; Daughtrey, Margery L.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Long Isl Hort Res & Extens Ctr, Riverhead, NY 11901 USA. RP Adkins, S (reprint author), ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM scott.adkins@ars.usda.gov FU USDA-NIFA Critical Issues [2011-37610-31178]; Florida Specialty Crop Block Grant [018014]; USDA-AFRI [2012-68004-20166]; Florida Tomato Committee; Florida Specialty Crop Foundation; Florida Fruit and Vegetable Association FX We thank C. Vanderspool, K. Bowers, A. Moreno, and B. Burns for their excellent technical assistance, numerous scouts and growers for help with sample collection, J. Marois for soybean seeds, and E. Rosskopf and N. Burelle for tomato seeds. Financial support was provided in part by grants from the USDA-NIFA Critical Issues (2011-37610-31178), Florida Specialty Crop Block Grant (018014) and USDA-AFRI (2012-68004-20166) programs, and the Florida Tomato Committee, the Florida Specialty Crop Foundation, and the Florida Fruit and Vegetable Association. Mention of a trademark, warranty, proprietary product, or vendor does not constitute a guarantee by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 40 TC 14 Z9 14 U1 0 U2 23 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2015 VL 105 IS 3 BP 388 EP 398 DI 10.1094/PHYTO-06-14-0172-R PG 11 WC Plant Sciences SC Plant Sciences GA CB8JA UT WOS:000349874100012 PM 25317844 ER PT J AU Ott, JP Hartnett, DC AF Ott, Jacqueline P. Hartnett, David C. TI Bud bank dynamics and clonal growth strategy in the rhizomatous grass, Pascopyrum smithii SO PLANT ECOLOGY LA English DT Article DE Bud; Guerilla; Phalanx; Rhizome; Vegetative reproduction; Western wheatgrass ID WATER-USE EFFICIENCY; TALLGRASS PRAIRIE; APICAL DOMINANCE; RESPONSES; DEFOLIATION; GUERRILLA; PHALANX; REPENS; WHEATGRASS; DEMOGRAPHY AB Recruitment of rhizomatous perennial grass ramets primarily occurs from the belowground bud bank. Investment in guerilla versus phalanx growth is determined by bud availability, development, and spatial distribution. The tiller and bud bank dynamics of Pascopyrum smithii, a dominant rhizomatous grass of the northern mixed-grass prairie, were examined in South Dakota throughout an annual cycle to assess the investment in guerilla versus phalanx growth and the role of different bud populations in renewal versus regenerative functions and vegetative spread via rhizomes. Pascopyrum smithii invested substantially in both phalanx and guerilla tiller production. However, investment in rhizome production before tiller recruitment prioritized guerilla over phalanx growth. Annual tiller recruitment of P. smithii was capable of flexible timing, occurring in either spring or fall. Renewal buds, from which rhizomes and tillers were recruited, primarily consisted of the youngest generation of buds borne at the base of tillers. Although rhizome axillary buds and older tiller axillary buds were rarely used in annual tiller recruitment, they provided a sizable reserve (regenerative) bud bank. The spatial distribution of bud development produced the mixed guerrilla-phalanx growth pattern and flexible tiller recruitment timing of P. smithii. Therefore, P. smithii is capable of employing both conservative and foraging growth strategies which will facilitate its persistence under local neighborhood variability and changing resource availability associated with environmental change. Understanding the spatial distribution of buds as determined by rhizome architecture is essential to understanding the distribution and composition of species within plant communities dominated by clonal species. C1 [Ott, Jacqueline P.; Hartnett, David C.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. RP Ott, JP (reprint author), US Forest Serv Rocky Mt Res Stn, Forest & Grassland Res Lab, Rapid City, SD 57702 USA. EM jacquelinepott@fs.fed.us FU Kansas State University Division of Biology; Konza Prairie Long-Term Ecological Research Program; USDA Rangeland Research Program [310306] FX This work was supported by the Kansas State University Division of Biology, the Konza Prairie Long-Term Ecological Research Program, and the USDA Rangeland Research Program [Grant number 310306]. We thank C. Ferguson, J. Nippert, and L. Murray for helpful suggestions on previous drafts of this manuscript. We also thank K. Sebes for lab assistance, and Wind Cave NP, especially B. Burkhart, for their field site support. NR 42 TC 1 Z9 2 U1 7 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD MAR PY 2015 VL 216 IS 3 BP 395 EP 405 DI 10.1007/s11258-014-0444-6 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CB9TF UT WOS:000349975000004 ER PT J AU Spadoni, A Guidarelli, M Phillips, J Mari, M Wisniewski, M AF Spadoni, A. Guidarelli, M. Phillips, J. Mari, M. Wisniewski, M. TI Transcriptional profiling of apple fruit in response to heat treatment: Involvement of a defense response during Penicillium expansum infection SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Blue mold; Microarray analysis; Gene expression; Hot Water Treatment ID HOT-WATER TREATMENT; SHOCK-PROTEIN; NEONECTRIA-GALLIGENA; EPICUTICULAR WAX; CALCIUM-UPTAKE; STORAGE ROTS; EXPRESSION; STRESS; ARABIDOPSIS; RESISTANCE AB Heat treatment of harvested fruit has been demonstrated to be an effective and a safe approach for managing postharvest decay. In the present study, the effect of a hot water treatment (HT) (45 degrees C for 10 min) on the response of apple to blue mold infection was investigated. HT was applied to 'Ultima Gala' apples using 2 different methods. Wounded apples were: (1) inoculated with a Penicillium expansum spore suspension and then heat-treated after I, 4 and 24 h (Inoc-HT); or (2) first heat-treated and then inoculated with a P. expansum spore suspension after 1, 4 and 24 h (HT-Inoc). All treated/inoculated apples were stored at 20 degrees C for 6 days. Significant reductions in fruit rot incidence, up to 100%, were observed using the Inoc-HT protocol at 4 and 24 h while a 30% reduction in blue mold incidence was found at 1 and 4 h using the HT-Inoc method. In vitro experiments showed no evident lethal effect of HT at 45 degrees C for 10 min on the germination of P. expansum conidia, indicating that this pathogen has a high heat tolerance. In order to investigate the molecular mechanisms involved in fruit response to heat treatment, an apple microarray was used to conduct a global transcriptional analysis of gene expression in apple at 0, 15,30 min, 1, 4, 8 and 24 h after the heat treatment. The results provided evidence that at 1 and 4 h after heating, the HT apples had the highest number of differentially expressed genes. A significant upregulation of heat shock proteins, heat shock cognate protein, and heat shock transcription factor genes, involved in thermotolerance were observed. This indicates that the apple fruit respond to the heat treatment in a programmed manner and suggests that the genes responsible for thermotolerance may also be involved in the induced resistance response. Published by Elsevier B.V. C1 [Spadoni, A.; Guidarelli, M.; Mari, M.] Univ Bologna, Dept Agr Sci, Alma Mater Studiorum, I-40127 Bologna, Italy. [Phillips, J.] USDA ARS, Eastern Reional Res Ctr, Wyndmoor, PA 25430 USA. [Wisniewski, M.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Wisniewski, M (reprint author), USDA ARS, Appalachian Fruit Res Stn, Wiltshire Rd 2217, Kearneysville, WV 25430 USA. NR 38 TC 10 Z9 10 U1 3 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2015 VL 101 BP 37 EP 48 DI 10.1016/j.postharybio.2014.10.009 PG 12 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CC1FQ UT WOS:000350087000006 ER PT J AU Lindsey, RL Fedorka-Cray, PJ Abley, M Turpin, JB Meinersmann, RJ AF Lindsey, Rebecca L. Fedorka-Cray, Paula J. Abley, Melanie Turpin, Jennifer B. Meinersmann, Richard J. TI Evaluating the Occurrence of Escherichia albertii in Chicken Carcass Rinses by PCR, Vitek Analysis, and Sequencing of the rpoB Gene SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RIBOSOMAL-RNA GENE; HAFNIA-ALVEI; MOLECULAR-IDENTIFICATION; COLI-STRAINS; CORYNEBACTERIUM; GASTROENTERITIS; PHYLOGENY; PATHOGEN; OUTBREAK; MEMBERS AB Escherichia albertii is a recently described species that has been associated with gastroenteritis in humans and with healthy and ill birds. Most recently, it has been identified as the causative agent in a food-borne outbreak in Japan. The distribution and clinical importance of E. albertii are not well studied because its importance is unclear. Culture methods for clinical isolation frequently miss E. albertii or incorrectly identify it as Shigella spp., Escherichia coli, or Hafnia alvei. This study was designed to determine if E. albertii could be recovered from chicken carcass rinses collected at slaughter during a 1-year period from November 2009 until October 2010. Colonies were isolated from chicken carcass rinses and tested by PCR for the presence or absence of clpX, lysP, mdh, intimin (eae), Shiga toxins 1 and 2 (stx(1), stx(2), and stx(2f)), heat-stable enterotoxin A (staA), and cytolethal distending toxins 1 and 2 (cdtB) genes. Sixty-five isolates were analyzed by sequencing a section of the rpoB gene. Analysis of the rpoB gene sequences revealed 14 fixed differences between E. albertii and other, closely related organisms. The fixed differences found in the rpoB gene could aid in future discrimination of E. albertii from closely related bacteria. C1 [Lindsey, Rebecca L.; Fedorka-Cray, Paula J.; Abley, Melanie; Turpin, Jennifer B.; Meinersmann, Richard J.] USDA, Richard B Russell Agr Res Ctr, Agr Res Serv, Bacterial Epidemiol & Antimicrobial Resistance Re, Athens, GA 30601 USA. RP Meinersmann, RJ (reprint author), USDA, Richard B Russell Agr Res Ctr, Agr Res Serv, Bacterial Epidemiol & Antimicrobial Resistance Re, Athens, GA 30601 USA. EM rick.meinersmann@ars.usda.gov NR 42 TC 2 Z9 2 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2015 VL 81 IS 5 BP 1727 EP 1734 DI 10.1128/AEM.03681-14 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CB3RY UT WOS:000349547200021 PM 25548040 ER PT J AU Morin, RS Randolph, KC Steinman, J AF Morin, Randall S. Randolph, KaDonna C. Steinman, Jim TI Mortality rates associated with crown health for eastern forest tree species SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Tree crown health; Tree mortality; Forest inventory; Adelges tsugae; Beech bark disease; Forest health ID BEECH BARK DISEASE; UNITED-STATES; PINE DECLINE; GROWTH; DIEBACK AB The condition of tree crowns is an important indicator of tree and forest health. Crown conditions have been evaluated during inventories of the US Forest Service Forest Inventory and Analysis (FIA) program since 1999. In this study, remeasured data from 55,013 trees on 2616 FIA plots in the eastern USAwere used to assess the probability of survival among various tree species using the suite of FIA crown condition variables. Logistic regression procedures were employed to develop models for predicting tree survival. Results of the regression analyses indicated that crown dieback was the most important crown condition variable for predicting tree survival for all species combined and for many of the 15 individual species in the study. The logistic models were generally successful in representing recent tree mortality responses to multiyear infestations of beech bark disease and hemlock woolly adelgid. Although our models are only applicable to trees growing in a forest setting, the utility of models that predict impending tree mortality goes beyond forest inventory or traditional forestry growth and yield models and includes any application where managers need to assess tree health or predict tree mortality including urban forest, recreation, wildlife, and pest management. C1 [Morin, Randall S.] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. [Randolph, KaDonna C.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN 37919 USA. [Steinman, Jim] US Forest Serv, USDA, Northeastern Area State & Private Forestry, Newtown Sq, PA 19073 USA. RP Morin, RS (reprint author), US Forest Serv, USDA, No Res Stn, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. EM rsmorin@fs.fed.us NR 45 TC 0 Z9 0 U1 2 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD MAR PY 2015 VL 187 IS 3 AR 87 DI 10.1007/s10661-015-4332-x PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CB2CS UT WOS:000349434900032 PM 25655130 ER PT J AU Metson, GS Iwaniec, DM Baker, LA Bennett, EM Childers, DL Cordell, D Grimm, NB Grove, JM Nidzgorski, DA White, S AF Metson, Genevieve S. Iwaniec, David M. Baker, Lawrence A. Bennett, Elena M. Childers, Daniel L. Cordell, Dana Grimm, Nancy B. Grove, J. Morgan Nidzgorski, Daniel A. White, Stuart TI Urban phosphorus sustainability: Systemically incorporating social, ecological, and technological factors into phosphorus flow analysis SO ENVIRONMENTAL SCIENCE & POLICY LA English DT Article DE Phosphorus; Substance flow analysis; Urban ecology; Urban sustainability ID WATER MANAGEMENT; DECISION-MAKING; SYSTEMS; SCIENCE; POLICY; CONSUMPTION; GOVERNANCE; INDICATORS; FRAMEWORK; ECOSYSTEM AB Phosphorus (P) is an essential fertilizer for agricultural production but is also a potent aquatic pollutant. Current P management fails to adequately address both the issue of food security due to P scarcity and P pollution threats to water bodies. As centers of food consumption and waste production, cities transport and store much P and thus provide important opportunities to improve P management. Substance flow analysis (SFA) is often used to understand urban P cycling and to identify inefficiencies that may be improved on. However, SFAs typically do not examine the factors that drive observed P dynamics. Understanding the social, ecological, and technological context of P stocks and flows is necessary to link urban P management to existing urban priorities and to select local management options that minimize tradeoffs and maximize synergies across priorities. Here, we review P SFA studies in 18 cities, focusing on gaps in the knowledge required to implement P management solutions. We develop a framework to systemically explore the full suite of factors that drive P dynamics in urban systems. By using this framework, scientists and managers can build a better understanding of the drivers of P cycling and improve our ability to address unsustainable P use and waste. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Metson, Genevieve S.; Bennett, Elena M.] McGill Univ, Dept Nat Resource Sci, Ste Anne De Bellevue, PQ, Canada. [Metson, Genevieve S.; Bennett, Elena M.] McGill Univ, McGill Sch Environm, Ste Anne De Bellevue, PQ, Canada. [Iwaniec, David M.; Grimm, Nancy B.] Arizona State Univ, Julie Ann Wrigley Global Inst Sustainabil, Tempe, AZ 85287 USA. [Baker, Lawrence A.] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA. [Childers, Daniel L.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Cordell, Dana; White, Stuart] Univ Technol Sydney, Inst Sustainable Futures, Sydney, NSW 2007, Australia. [Grimm, Nancy B.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Grove, J. Morgan] US Forest Serv, No Res Stn, Baltimore, MD USA. [Nidzgorski, Daniel A.] Univ Minnesota, St Paul, MN 55108 USA. RP Metson, GS (reprint author), McGill Univ, Dept Nat Resource Sci, 21,111 Lakeshore Rd, Ste Anne De Bellevue, PQ, Canada. EM genevieve.metson@mail.mcgill.ca; diwaniec@asu.edu; baker127@umn.edu; elena.bennett@mcgill.ca; dan.childers@asu.edu; dana.cordell@uts.edu.au; nbgrimm@asu.edu; jmgrove@gmail.com; dnidz@umn.edu; Stuart.White@uts.edu.au RI Grimm, Nancy/D-2840-2009; Iwaniec, David/M-7993-2014; OI Grimm, Nancy/0000-0001-9374-660X; Iwaniec, David/0000-0002-0410-4152; White, Stuart/0000-0001-8078-5327; Nidzgorski, Daniel/0000-0003-4454-3042 FU U.S. National Science Foundation (NSF) through the Urban Sustainability Research Coordination Network [1140070]; NSERC [RGPIN 327077]; NSF through the Central Arizona-Phoenix Long-Term Ecological Research Program [1026865] FX Support was provided by the U.S. National Science Foundation (NSF) through the Urban Sustainability Research Coordination Network (Grant No. 1140070) and provided by NSERC through a Discovery Grant (Grant No. RGPIN 327077) to EMB. DMI, DLC, and NBG also received support from the NSF through the Central Arizona-Phoenix Long-Term Ecological Research Program (Grant No. 1026865). NR 57 TC 8 Z9 10 U1 4 U2 52 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1462-9011 EI 1873-6416 J9 ENVIRON SCI POLICY JI Environ. Sci. Policy PD MAR PY 2015 VL 47 BP 1 EP 11 DI 10.1016/j.envsci.2014.10.005 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CB4EQ UT WOS:000349581400001 ER PT J AU Versmissen, J Oosterveer, DM Yazdanpanah, M Dehghan, A Holm, H Erdman, J Aulchenko, YS Thorleifsson, G Schunkert, H Huijgen, R Vongpromek, R Uitterlinden, AG Defesche, JC van Duijn, CM Mulder, M Dadd, T Karlsson, HD Ordovas, J Kindt, I Jarman, A Hofman, A van Vark-van der Zee, L Blommesteijn-Touw, AC Kwekkeboom, J Liem, AH van der Ouderaa, FJ Calandra, S Bertolini, S Averna, M Langslet, G Ose, L Ros, E Almagro, F de Leeuw, PW Civeira, F Masana, L Pinto, X Simoons, ML Schinkel, AFL Green, MR Zwinderman, AH Johnson, KJ Schaefer, A Neil, A Witteman, JCM Humphries, SE Kastelein, JJP Sijbrands, EJG AF Versmissen, Jorie Oosterveer, Daniella M. Yazdanpanah, Mojgan Dehghan, Abbas Holm, Hilma Erdman, Jeanette Aulchenko, Yurii S. Thorleifsson, Gudmar Schunkert, Heribert Huijgen, Roeland Vongpromek, Ranitha Uitterlinden, Andre G. Defesche, Joep C. van Duijn, Cornelia M. Mulder, Monique Dadd, Tony Karlsson, Hrobjartur D. Ordovas, Jose Kindt, Iris Jarman, Amelia Hofman, Albert van Vark-van der Zee, Leonie Blommesteijn-Touw, Adriana C. Kwekkeboom, Jaap Liem, Anho H. van der Ouderaa, Frans J. Calandra, Sebastiano Bertolini, Stefano Averna, Maurizio Langslet, Gisle Ose, Leiv Ros, Emilio Almagro, Fatima de Leeuw, Peter W. Civeira, Fernando Masana, Luis Pinto, Xavier Simoons, Maarten L. Schinkel, Arend F. L. Green, Martin R. Zwinderman, Aeilko H. Johnson, Keith J. Schaefer, Arne Neil, Andrew Witteman, Jacqueline C. M. Humphries, Steve E. Kastelein, John J. P. Sijbrands, Eric J. G. TI Identifying genetic risk variants for coronary heart disease in familial hypercholesterolemia: an extreme genetics approach SO EUROPEAN JOURNAL OF HUMAN GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; ARTERY-DISEASE; CARDIOVASCULAR-DISEASE; QUANTITATIVE TRAITS; POLYMORPHISMS; POPULATION; PCSK9; LOCI; LDL AB Mutations in the low-density lipoprotein receptor (LDLR) gene cause familial hypercholesterolemia (FH), a disorder characterized by coronary heart disease (CHD) at young age. We aimed to apply an extreme sampling method to enhance the statistical power to identify novel genetic risk variants for CHD in individuals with FH. We selected cases and controls with an extreme contrast in CHD risk from 17 000 FH patients from the Netherlands, whose functional LDLR mutation was unequivocally established. The genome-wide association (GWA) study was performed on 249 very young FH cases with CHD and 217 old FH controls without CHD (above 65 years for males and 70 years of age for females) using the Illumina HumanHap550K chip. In the next stage, two independent samples (one from the Netherlands and one from Italy, Norway, Spain, and the United Kingdom) of FH patients were used as replication samples. In the initial GWA analysis, we identified 29 independent single nucleotide polymorphisms (SNPs) with suggestive associations with premature CHD (P<1 x 10(-4)). We examined the association of these SNPs with CHD risk in the replication samples. After Bonferroni correction, none of the SNPs either replicated or reached genome-wide significance after combining the discovery and replication samples. Therefore, we conclude that the genetics of CHD risk in FH is complex and even applying an 'extreme genetics' approach we did not identify new genetic risk variants. Most likely, this method is not as effective in leveraging effect size as anticipated, and may, therefore, not lead to significant gains in statistical power. C1 [Versmissen, Jorie; Oosterveer, Daniella M.; Yazdanpanah, Mojgan; Vongpromek, Ranitha; Uitterlinden, Andre G.; Mulder, Monique; van Vark-van der Zee, Leonie; Blommesteijn-Touw, Adriana C.; Schinkel, Arend F. L.; Sijbrands, Eric J. G.] Erasmus Univ, Med Ctr, Dept Internal Med, Rotterdam, Netherlands. [Dehghan, Abbas; Aulchenko, Yurii S.; Uitterlinden, Andre G.; van Duijn, Cornelia M.; Hofman, Albert; Witteman, Jacqueline C. M.] Erasmus Univ, Med Ctr, Dept Epidemiol, Rotterdam, Netherlands. [Holm, Hilma; Thorleifsson, Gudmar] deCODE Genet, Reykjavik, Iceland. [Erdman, Jeanette; Schunkert, Heribert] Tech Univ Munich, Deutsch Hertzzentrum Munchen, D-80290 Munich, Germany. [Aulchenko, Yurii S.] Novosibirsk State Univ, Novosibirsk 630090, Russia. [Huijgen, Roeland; Defesche, Joep C.; Kastelein, John J. P.] Amsterdam Med Ctr, Dept Vasc Med, Amsterdam, Netherlands. [Dadd, Tony; Jarman, Amelia; Green, Martin R.] Unilever R&D, Sharnbrook, Beds, England. [Karlsson, Hrobjartur D.] Landspitali Univ Hosp, Iceland Heart Assoc, Reykjavik, Iceland. [Karlsson, Hrobjartur D.] Landspitali Univ Hosp, Dept Cardiol, Reykjavik, Iceland. [Ordovas, Jose] Tufts Univ, Jean Mayer USDA HNRCA, Nutr & Genom Lab, Boston, MA 02111 USA. [Kindt, Iris] Stichting Opsporing Erfelijke Hypercholesterolemi, Amsterdam, Netherlands. [Kwekkeboom, Jaap] Erasmus Univ, Med Ctr, Dept Gastroenterol & Hepatol, Rotterdam, Netherlands. [Liem, Anho H.] St Franciscus Gasthuis Hosp, Dept Cardiol, Rotterdam, Netherlands. [van der Ouderaa, Frans J.] Leiden Univ, Med Ctr, Netherlands Consortium Hlth Ageing, Leiden, Netherlands. [Calandra, Sebastiano] Univ Modena & Reggio Emilia, Dept Biomed Sci, Modena, Italy. [Bertolini, Stefano] Univ Genoa, Dept Internal Med, I-16126 Genoa, Italy. [Averna, Maurizio] Univ Palermo, Dept Internal Med & Med Specialties, Palermo, Italy. [Langslet, Gisle; Ose, Leiv] Oslo Univ Hosp, Lipid Clin, Oslo, Norway. [Ros, Emilio] Hosp Clin Barcelona, IDIBAPS, Lipid Clin, Endocrinol & Nutr Serv, Barcelona, Spain. [Ros, Emilio] ISCIII, CIBER Fisiopatol Obesidad & Nutr CIBERobn, Madrid, Spain. [Almagro, Fatima] Hosp Guipuzcoa, San Sebastian, Guipuzcoa, Spain. [de Leeuw, Peter W.] Maastricht Univ, Med Ctr, Dept Internal Med, NL-6200 MD Maastricht, Netherlands. [Civeira, Fernando] Hosp Univ Miguel Servet, Lipids Unit, Zaragoza, Spain. [Civeira, Fernando] Hosp Univ Miguel Servet, Mol Res Unit, Zaragoza, Spain. [Masana, Luis] Hosp Univ St Joan Reus, URLA, Reus, Spain. [Pinto, Xavier] Hosp Univ Bellvitge, Serv Med Interna, Unitat Lipids & Arteriosclerosi, Barcelona, Spain. [Simoons, Maarten L.; Schinkel, Arend F. L.] Erasmus Univ, Med Ctr, Dept Cardiol, Rotterdam, Netherlands. [Zwinderman, Aeilko H.] Amsterdam Med Ctr, Dept Clin Epidemiol & Biostat, Amsterdam, Netherlands. [Johnson, Keith J.] Novartis Insitutes Biomed Res, Cambridge, MA USA. [Schaefer, Arne] Univ Kiel, Inst Clin Mol Biol, Kiel, Germany. [Neil, Andrew] Univ Oxford, Oxford Ctr Diabet Endocrinol & Metab, Oxford, England. [Humphries, Steve E.] UCL, Inst Cardiovasc Sci, BHF Labs, London, England. RP Sijbrands, EJG (reprint author), Erasmus MC, Dr Molewaterpl 10, NL-3015 GD Rotterdam, Netherlands. EM e.sijbrands@erasmusmc.nl RI Aulchenko, Yurii/M-8270-2013; OI Aulchenko, Yurii/0000-0002-7899-1575; Sijbrands, Eric/0000-0001-8857-7389; Masana, Luis/0000-0002-0789-4954; Averna, Maurizio/0000-0003-3558-9209; Dehghan, Abbas/0000-0001-6403-016X; Humphries, Stephen E/0000-0002-8221-6547 FU Dutch Heart Foundation [2006B190]; Unilever, UK; Pfizer; MSD The Netherlands; Department of Health's NIHR Biomedical Research Centre [RG 2008/008]; ISCIII, Spain [FIS PS09/01292] FX We thank Carl Jarman and David Gunn for help in sample preparation and data collection. CIBERobn is an initiative of ISCIII, Spain. This work was supported by the Dutch Heart Foundation (2006B190) and Unilever, UK. Additional funding was provided by Pfizer and MSD The Netherlands. SEH acknowledges BHF support (RG 2008/008) and also funding from the Department of Health's NIHR Biomedical Research Centre funding scheme. ER is supported by grant FIS PS09/01292 from ISCIII, Spain. NR 28 TC 5 Z9 5 U1 0 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1018-4813 EI 1476-5438 J9 EUR J HUM GENET JI Eur. J. Hum. Genet. PD MAR PY 2015 VL 23 IS 3 BP 381 EP 387 DI 10.1038/ejhg.2014.101 PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA CB5MG UT WOS:000349670900017 PM 24916650 ER PT J AU Paul, L Jacques, PF Aviv, A Vasan, RS D'Agostino, RB Levy, D Selhub, J AF Paul, Ligi Jacques, Paul F. Aviv, Abraham Vasan, Ramachandran S. D'Agostino, Ralph B. Levy, Daniel Selhub, Jacob TI High plasma folate is negatively associated with leukocyte telomere length in Framingham Offspring cohort SO EUROPEAN JOURNAL OF NUTRITION LA English DT Article DE Telomere length; Folate; Multivitamins; Folic acid fortification ID FOLIC-ACID SUPPLEMENTATION; BREAST-CANCER; ALZHEIMERS-DISEASE; PERNICIOUS-ANEMIA; HUMAN FIBROBLASTS; MULTIVITAMIN USE; MAMMALIAN-CELLS; RISK; HOMOCYSTEINE; METABOLISM AB Shortening of telomeres, the protective structures at the ends of eukaryotic chromosomes, is associated with age-related pathologies. Telomere length is influenced by DNA integrity and DNA and histone methylation. Folate plays a role in providing precursors for nucleotides and methyl groups for methylation reactions and has the potential to influence telomere length. We determined the association between leukocyte telomere length and long-term plasma folate status (mean of 4 years) in Framingham Offspring Study (n = 1,044, females = 52.1 %, mean age 59 years) using data from samples collected before and after folic acid fortification. Leukocyte telomere length was determined by Southern analysis and fasting plasma folate concentration using microbiological assay. There was no significant positive association between long-term plasma folate and leukocyte telomere length among the Framingham Offspring Study participants perhaps due to their adequate folate status. While the leukocyte telomere length in the second quintile of plasma folate was longer than that in the first quintile, the difference was not statistically significant. The leukocyte telomere length of the individuals in the fifth quintile of plasma folate was shorter than that of those in the second quintile by 180 bp (P < 0.01). There was a linear decrease in leukocyte telomere length with higher plasma folate concentrations in the upper four quintiles of plasma folate (P for trend = 0.001). Multivitamin use was associated with shorter telomeres in this cohort (P = 0.015). High plasma folate status possibly resulting from high folic acid intake may interfere with the role of folate in maintaining telomere integrity. C1 [Paul, Ligi; Jacques, Paul F.; Selhub, Jacob] Tufts Univ, JM USDA HNRC, Boston, MA 02111 USA. [Aviv, Abraham] Univ Med & Dent New Jersey, Ctr Human Dev & Aging, Newark, NJ 07103 USA. [Vasan, Ramachandran S.; D'Agostino, Ralph B.; Levy, Daniel] NHLBI, Framingham Heart Study, Framingham, MA 01702 USA. RP Paul, L (reprint author), Tufts Univ, JM USDA HNRC, 711 Washington St, Boston, MA 02111 USA. EM Ligi.Paul_Pottenplackel@tufts.edu OI Ramachandran, Vasan/0000-0001-7357-5970 FU United States Department of Agriculture [51520-008-04S]; National Heart Lung and Blood Institute, Framingham Heart Study (NHLBI/NIH) [N01-HC-25195]; Boston University School of Medicine FX Support from United States Department of Agriculture Cooperative Agreement 51520-008-04S, National Heart Lung and Blood Institute, Framingham Heart Study (NHLBI/NIH Contract #N01-HC-25195) and Boston University School of Medicine. Any opinions, findings, conclusion or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the United States Department of Agriculture. NR 51 TC 5 Z9 5 U1 2 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-6207 EI 1436-6215 J9 EUR J NUTR JI Eur. J. Nutr. PD MAR PY 2015 VL 54 IS 2 BP 235 EP 241 DI 10.1007/s00394-014-0704-1 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CB6WF UT WOS:000349766800008 PM 24793435 ER PT J AU Zhang, X Perez-Rodriguez, P Semagn, K Beyene, Y Babu, R Lopez-Cruz, MA Vicente, FS Olsen, M Buckler, E Jannink, JL Prasanna, BM Crossa, J AF Zhang, X. Perez-Rodriguez, P. Semagn, K. Beyene, Y. Babu, R. Lopez-Cruz, M. A. Vicente, F. San Olsen, M. Buckler, E. Jannink, J-L Prasanna, B. M. Crossa, J. TI Genomic prediction in biparental tropical maize populations in water-stressed and well-watered environments using low-density and GBS SNPs SO HEREDITY LA English DT Article ID QUANTITATIVE TRAITS; BREEDING POPULATIONS; MOLECULAR MARKERS; GENETIC VALUES; SELECTION; PEDIGREE; ACCURACY; GENOTYPE AB One of the most important applications of genomic selection in maize breeding is to predict and identify the best untested lines from biparental populations, when the training and validation sets are derived from the same cross. Nineteen tropical maize biparental populations evaluated in multienvironment trials were used in this study to assess prediction accuracy of different quantitative traits using low-density (similar to 200 markers) and genotyping-by-sequencing (GBS) single-nucleotide polymorphisms (SNPs), respectively. An extension of the Genomic Best Linear Unbiased Predictor that incorporates genotype x environment (GE) interaction was used to predict genotypic values; cross-validation methods were applied to quantify prediction accuracy. Our results showed that: (1) low-density SNPs (similar to 200 markers) were largely sufficient to get good prediction in biparental maize populations for simple traits with moderate-to-high heritability, but GBS outperformed low-density SNPs for complex traits and simple traits evaluated under stress conditions with low-to-moderate heritability; (2) heritability and genetic architecture of target traits affected prediction performance, prediction accuracy of complex traits (grain yield) were consistently lower than those of simple traits (anthesis date and plant height) and prediction accuracy under stress conditions was consistently lower and more variable than under well-watered conditions for all the target traits because of their poor heritability under stress conditions; and (3) the prediction accuracy of GE models was found to be superior to that of non-GE models for complex traits and marginal for simple traits. C1 [Zhang, X.; Lopez-Cruz, M. A.; Vicente, F. San; Olsen, M.; Crossa, J.] CIMMYT, Int Maize & Wheat Improvement Ctr, Mexico City 06600, DF, Mexico. [Perez-Rodriguez, P.] Colegio Postgrad, Montecillo, Estado De Mexic, Mexico. [Semagn, K.; Beyene, Y.; Prasanna, B. M.] CIMMYT, Int Maize & Wheat Improvement Ctr, Nairobi, Kenya. [Babu, R.] CIMMYT India, ICRISAT, Hyderabad, Andhra Pradesh, India. [Buckler, E.; Jannink, J-L] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. RP Crossa, J (reprint author), CIMMYT, Biometr & Stat Unit, Int Maize & Wheat Improvement Ctr, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. EM j.crossa@cgiar.org OI Semagn, Kassa/0000-0001-6486-5685; Crossa, Jose/0000-0001-9429-5855; Buckler, Edward/0000-0002-3100-371X FU Water Effective Maize for Africa (WEMA) project; Cornell-CIMMYT Genomic Selection project - Bill and Melinda Gates Foundation FX We thank all collaborators in Africa who were involved in conducting the extensive biparental multienvironment trials. We acknowledge the financial support provided by the Water Effective Maize for Africa (WEMA) project and the Cornell-CIMMYT Genomic Selection project financed by the Bill and Melinda Gates Foundation. NR 34 TC 22 Z9 23 U1 0 U2 35 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD MAR PY 2015 VL 114 IS 3 BP 291 EP 299 DI 10.1038/hdy.2014.99 PG 9 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA CB5MH UT WOS:000349671000006 PM 25407079 ER PT J AU Ballou, MA Hanson, DL Cobb, CJ Obeidat, BS Sellers, MD Pepper-Yowell, AR Carroll, JA Earleywine, TJ Lawhon, SD AF Ballou, M. A. Hanson, D. L. Cobb, C. J. Obeidat, B. S. Sellers, M. D. Pepper-Yowell, A. R. Carroll, J. A. Earleywine, T. J. Lawhon, S. D. TI Plane of nutrition influences the performance, innate leukocyte responses, and resistance to an oral Salmonella enterica serotype Typhimurium challenge in Jersey calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE calf; health; immune; plane of nutrition ID FED MILK REPLACERS; IMMUNE-RESPONSES; DAIRY CALVES; BODY-COMPOSITION; NEONATAL CALVES; GROWTH; HOLSTEIN; PROTEIN; HEALTH AB Two experiments investigated how plane of nutrition influences performance, leukocyte responses, and resistance to an oral Salmonella enterica serotype Typhimurium challenge. In experiment 1, 46 (2 +/- 1 d of age) calves were randomly assigned to 2 diets: a low (LPN; n = 23) and high plane of nutrition (HPN; n 23). The LPN calves were fed 409 g/d of dry matter (DM) of a 20% crude protein and 20% fat milk replacer, whereas HPN calves were fed 610 and 735 g/d of DM of a 28% crude protein and 25% fat milk replacer during wk 1 and 2 to 6, respectively. In experiment 2, 20 bull calves (LPN; n = 11 and HPN; n = 9) were orally challenged on d 80 with 1.5 x 10(7) cfu of Salmonella Typhimurium (ATCC #14028). The HPN calves had a greater incidence (87.5 vs. 45.5%) and duration of days with high fecal scores (5.5 vs. 3.5 d). The LPN calves had greater neutrophil surface expression of L-selectin on d 7, 21, and 42. Following the Salmonella Typhimurium challenge, calf starter DM intake was greater among the HPN calves. The percentage of neutrophils producing an oxidative burst was also greater among HPN calves on d 1 to 5 after the challenge. Similarly, the intensity of the oxidative burst tended to be greater among the HPN calves on d 2 and 3 postchallenge. The secretion of tumor necrosis factor-la from whole-blood cultures stimulated with lipopolysaccharide tended to be greater on d 1 and was greater on d 5 and 6 among HPN calves. The median ranks of haptoglobin concentrations were greater and plasma zinc concentrations tended to be decreased among LPN calves. These data indicate that feeding a HPN to Jersey calves improved average daily gain and feed efficiency, but increased the incidence of high fecal scores during the first few weeks of life; however, the HPN Jersey calves may be more resistant to Salmonella Typliimurium after weaning. C1 [Ballou, M. A.; Hanson, D. L.; Cobb, C. J.; Obeidat, B. S.; Sellers, M. D.; Pepper-Yowell, A. R.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. [Obeidat, B. S.] Jordan Univ Sci & Technol, Fac Agr, Dept Anim Prod, Irbid 22110, Jordan. [Carroll, J. A.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79401 USA. [Earleywine, T. J.] Land OLakes Milk Prod Co, Minneapolis, MN 55126 USA. [Lawhon, S. D.] Texas A&M Univ, College Stn, TX 77845 USA. RP Ballou, MA (reprint author), Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. EM michael.ballou@ttu.edu RI Lawhon, Sara/G-5147-2011 OI Lawhon, Sara/0000-0001-9154-8909 NR 22 TC 6 Z9 6 U1 4 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2015 VL 98 IS 3 BP 1972 EP 1982 DI 10.3168/jds.2014-8783 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CB4FW UT WOS:000349584600053 PM 25597967 ER PT J AU Antaya, NT Soder, KJ Kraft, J Whitehouse, NL Guindon, NE Erickson, PS Conroy, AB Brito, AF AF Antaya, N. T. Soder, K. J. Kraft, J. Whitehouse, N. L. Guindon, N. E. Erickson, P. S. Conroy, A. B. Brito, A. F. TI Incremental amounts of Ascophyllum nodosum meal do not improve animal performance but do increase milk iodine output in early lactation dairy cows fed high-forage diets SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE Ascophyllum nodosum meal; dairy cow; iodine; milk yield ID SEAWEED EXTRACT; REPLACING ALFALFA; BROWN SEAWEED; HEAT-STRESS; FATTY-ACIDS; EXCRETION; TASCO; LAMBS; GLUCOCORTICOIDS; DIGESTIBILITY AB The objective of this study was to investigate the effects of incremental amounts of Ascophyllum nodosum meal (ANOD) on milk production, milk composition including fatty acids and I, blood metabolites, and nutrient intake and digestibility in early lactation dairy cows fed high-forage diets. Twelve multiparous Jersey cows averaging (mean +/- standard deviation) 40 +/- 21 d in milk and 464 +/- 35 kg of body weight and 4 primiparous Jersey cows averaging 75 +/- 37 d in milk and 384 +/- 17 kg of body weight were randomly assigned to treatment sequences in a replicated 4 x 4 Latin square design. Each period lasted 21 d with 14 d for diet adaptation and 7 d for data and sample collection. Cows were fed a total mixed ration (64:36 forage-to-concentrate ratio) supplemented (as fed) with 0, 57, 113, or 170 g/d of ANOD. Milk yield as well as concentrations and yields of milk components (fat, protein, lactose, milk urea N) were not affected by increasing dietary amounts of ANOD. Concentration (from 178 to 1,370 pg/L) and yield (from 2.8 to 20.6 mg/d) of milk I increased linearly in cows fed incremental amounts of ANOD as a result of the high concentration of I (820 mg/kg of dry matter) in ANOD. Overall, only minor changes were observed in the proportion of milk fatty acids with ANOD supplementation. Quadratic trends were observed for dry matter intake and total-tract digestibilities of organic matter and neutral detergent fiber, whereas negative linear trends were observed for serum concentration of cortisol and crude protein digestibility with ANOD supplementation. Serum concentrations of triiodothyronine and thyroxine were not affected by ANOD supplementation and averaged 1.1 and 48.4 ng/mL, respectively. However, feeding increasing amounts of ANOD linearly reduced the plasma concentration of nonesterified fatty acids (from 164 to 132 mEq/L). Quadratic effects were found for the total-tract digestibility of ADF and urinary output of purine derivatives, suggesting that ANOD supplementation may stimulate growth of ruminal cellulolytic bacteria in a dose-dependent fashion. In general, feeding incremental amounts of ANOD to early lactation dairy cows dramatically increased milk I concentration and output with no effect on animal performance. C1 [Antaya, N. T.; Whitehouse, N. L.; Guindon, N. E.; Erickson, P. S.; Brito, A. F.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Soder, K. J.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Kraft, J.] Univ Vermont, Dept Anim Sci, Burlington, VT 05405 USA. [Conroy, A. B.] Univ New Hampshire, Thompson Sch Appl Sci, Durham, NH 03824 USA. RP Brito, AF (reprint author), Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. EM andre.brito@unh.edu FU New Hampshire Agricultural Experiment Station; USDA National Institute of Food and Agriculture [NC-1042, NH00616-R, 1001855] FX Partial funding was provided by the New Hampshire Agricultural Experiment Station. This is Scientific Contribution Number 2588. This work was supported by the USDA National Institute of Food and Agriculture (Hatch Multistate NC-1042 Project Number NH00616-R; Project Accession Number 1001855).Gratitude is extended to Melissa Rubano (USDA-Agricultural Research Station, Pasture Systems and Watershed Management Research Unit) and Melissa Bainbridge (University of Vermont) for technical assistance, as well as University of New Hampshire undergraduate students Elizabeth Fletcher, Samantha Werner, Gina Soule, Adam Crowther, and Justin Stilwell for support during samples collection. We also thank Christiane Girard (Agriculture and Agri-Food Canada/Dairy and Swine Research and Development Centre; Sherbrooke, QC, Canada) for vitamin B analysis and Thorvin Inc. NR 63 TC 4 Z9 4 U1 3 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2015 VL 98 IS 3 BP 1991 EP 2004 DI 10.3168/jds.2014-8851 PG 14 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CB4FW UT WOS:000349584600055 PM 25547299 ER PT J AU Tempelman, RJ Spurlock, DM Coffey, M Veerkamp, RF Armentano, LE Weigel, KA de Haas, Y Staples, CR Connor, EE Lu, Y VandeHaar, MJ AF Tempelman, R. J. Spurlock, D. M. Coffey, M. Veerkamp, R. F. Armentano, L. E. Weigel, K. A. de Haas, Y. Staples, C. R. Connor, E. E. Lu, Y. VandeHaar, M. J. TI Heterogeneity in genetic and nongenetic variation and energy sink relationships for residual feed intake across research stations and countries SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE heritability; international study; random regression model; residual feed intake ID RANDOM REGRESSION-MODELS; DAIRY-CATTLE; LACTATION PERFORMANCE; EFFICIENCY; COWS; SELECTION; SUPPLEMENTATION; HERITABILITY; COMPONENTS; VARIANCE AB Our long-term objective is to develop breeding strategies for improving feed efficiency in dairy cattle. In this study, phenotypic data were pooled across multiple research stations to facilitate investigation of the genetic and nongenetic components of feed efficiency in Holstein cattle. Specifically, the heritability of residual feed intake (RFI) was estimated and heterogeneous relationships between RFI and traits relating to energy utilization were characterized across research stations. Milk, fat, protein, and lactose production converted to megacalories (milk energy; MilkE), dry matter intakes (DMI), and body weights (BW) were collected on 6,824 lactations from 4,893 Holstein cows from research stations in Scotland, the Netherlands, and the United States. Weekly DMI, recorded between 50 to 200 d in milk, was fitted as a linear function of MilkE, BW 75, and change in BW (Delta BW), along with parity, a fifth-order polynomial on days in milk (DIM), and the interaction between this polynomial and parity in a first-stage model. The residuals from this analysis were considered to be a phenotypic measure of RFI. Estimated partial regression coefficients of DMI on MilkE and on BW 75 ranged from 0.29 to 0.47 kg/Mcal for MilkE across research stations, whereas estimated partial regression coefficients on BW 75 ranged from 0.06 to 0.16kg/kg(0.75). Estimated partial regression coefficients on Delta BW ranged from 0.06 to 0.39 across stations. Heritabilities for country-specific RFI were based on fitting second-stage random regression models and ranged from 0.06 to 0.24 depending on DIM. The overall heritability estimate across all research stations and all DIM was 0.15 +/- 0.02, whereas an alternative analysis based on combining the first- and second-stage model as 1 model led to an overall heritability estimate of 0.18 +/- 0.02. Hence future genomic selection programs on feed efficiency appear to be promising; nevertheless, care should be taken to allow for potentially heterogeneous variance components and partial relationships between DMI and other energy sink traits across environments when determining RFI. C1 [Tempelman, R. J.; Lu, Y.; VandeHaar, M. J.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Spurlock, D. M.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Coffey, M.] Scottish Agr Coll, Anim & Vet Sci Grp, Easter Bush EH25 9RG, Midlothian, Scotland. [Veerkamp, R. F.] Wageningen UR Livestock Res, Anim Breeding & Genom Ctr, NL-6700 AH Wageningen, Netherlands. [Armentano, L. E.; Weigel, K. A.; de Haas, Y.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Staples, C. R.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Connor, E. E.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Tempelman, RJ (reprint author), Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. EM tempelma@msu.edu OI Coffey, Mike/0000-0003-4890-6218 FU USDA-NIFA [2011-68004-30340]; Scottish Government FX The authors acknowledge funding from USDA-NIFA Grant Number 2011-68004-30340. CRV (Arnhem, the Netherlands) and the Why Dry-project (Wageningen University, the Netherlands) are acknowledged for providing part of the Dutch data. UK data collection was funded by Scottish Government. NR 33 TC 11 Z9 11 U1 7 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2015 VL 98 IS 3 BP 2013 EP 2026 DI 10.3168/jds.2014-8510 PG 14 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CB4FW UT WOS:000349584600057 PM 25582589 ER PT J AU Wiggans, GR VanRaden, PM Cooper, TA AF Wiggans, G. R. VanRaden, P. M. Cooper, T. A. TI Technical note: Rapid calculation of genomic evaluations for new animals SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE genomic evaluation; genotype; continuous evaluation AB A method was developed to calculate preliminary genomic evaluations daily or weekly before the release of official monthly evaluations by processing only newly genotyped animals using estimates of single nucleotide polymorphism effects from the previous official evaluation. To minimize computing time, reliabilities and genomic inbreeding are not calculated, and fixed weights are used to combine genomic and traditional information. Correlations of preliminary and September official monthly evaluations for animals with genotypes that became usable after the extraction of genotypes for August 2014 evaluations were >0.99 for most Holstein traits. Correlations were lower for breeds with smaller population size. Earlier access to genomic evaluations benefits producers by enabling earlier culling decisions and genotyping laboratories by making workloads more uniform across the month. C1 [Wiggans, G. R.; VanRaden, P. M.; Cooper, T. A.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Wiggans, GR (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM George.Wiggans@ars.usda.gov NR 4 TC 6 Z9 6 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2015 VL 98 IS 3 BP 2039 EP 2042 DI 10.3168/jds.2014-8868 PG 4 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CB4FW UT WOS:000349584600060 PM 25557894 ER EF