FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Uden, DR Allen, CR Mitchell, RB Mccoy, TD Guan, QF AF Uden, Daniel R. Allen, Craig R. Mitchell, Rob B. McCoy, Tim D. Guan, Qingfeng TI Predicted avian responses to bioenergy development scenarios in an intensive agricultural landscape SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE cellulosic ethanol; climate; conservation reserve program; geographic information systems; grassland birds; rowcrops; scenario planning; switchgrass ID CONSERVATION RESERVE PROGRAM; GRASSLAND BIRDS; CELLULOSIC ETHANOL; TALLGRASS PRAIRIE; BREEDING BIRDS; UNITED-STATES; LAND-USE; SWITCHGRASS; BIOMASS; ENERGY AB Conversion of native prairie to agriculture has increased food and bioenergy production but decreased wildlife habitat. However, enrollment of highly erodible cropland in conservation programs has compensated for some grassland loss. In the future, climate change and production of second-generation perennial biofuel crops could further transform agricultural landscapes and increase or decrease grassland area. Switchgrass (Panicum virgatum) is an alternative biofuel feedstock that may be economically and environmentally superior to maize (Zea mays) grain for ethanol production on marginally productive lands. Switchgrass could benefit farmers economically and increase grassland area, but there is uncertainty as to how conversions between rowcrops, switchgrass monocultures and conservation grasslands might occur and affect wildlife. To explore potential impacts on grassland birds, we developed four agricultural land-use change scenarios for an intensively cultivated landscape, each driven by potential future climatic changes and ensuing irrigation limitations, ethanol demand, commodity prices, and continuation of a conservation program. For each scenario, we calculated changes in area for landcover classes and predicted changes in grassland bird abundances. Overall, birds responded positively to the replacement of rowcrops with switchgrass and negatively to the conversion of conservation grasslands to switchgrass or rowcrops. Landscape context and interactions between climate, crop water use, and irrigation availability could influence future land-use, and subsequently, avian habitat quality and quantity. Switchgrass is likely to provide higher quality avian habitat than rowcrops but lower quality habitat than conservation grasslands, and therefore, may most benefit birds in heavily cultivated, irrigation dependent landscapes under warmer and drier conditions, where economic profitability may also encourage conversions to drought tolerant bioenergy feedstocks. C1 [Uden, Daniel R.] Univ Nebraska, Sch Nat Resources, Nebraska Cooperat Fish & Wildlife Res Unit, Lincoln, NE 68583 USA. [Allen, Craig R.] Univ Nebraska, Sch Nat Resources, Nebraska Cooperat Fish & Wildlife Res Unit, US Geol Survey, Lincoln, NE 68583 USA. [Mitchell, Rob B.] Univ Nebraska, Forage & Bioenergy Res Unit, USDA ARS Grain, Lincoln, NE 68583 USA. [McCoy, Tim D.] Nebraska Game & Pk Commiss, Lincoln, NE 68503 USA. [Guan, Qingfeng] China Univ Geosci Wuhan, Fac Informat Engn, Wuhan 430074, Hubei, Peoples R China. RP Uden, DR (reprint author), Univ Nebraska, Sch Nat Resources, Nebraska Cooperat Fish & Wildlife Res Unit, 3310 Holdrege St, Lincoln, NE 68583 USA. EM danielruden87@gmail.com FU Climate Effects Network; US Geological Survey; Nebraska Game and Parks Commission; University of Nebraska-Lincoln; US Fish and Wildlife Service; Wildlife Management Institute FX The authors thank Dr. Jennifer Smith and two anonymous reviewers for their contributions to this manuscript, the Rainwater Basin Joint Venture for providing GIS data, and the Great Plains Landscape Conservation Cooperative and US Geological Survey. Climate Effects Network for financial support. The Nebraska Cooperative Fish and Wildlife Research Unit is jointly supported by a cooperative agreement between the US Geological Survey, the Nebraska Game and Parks Commission, the University of Nebraska-Lincoln, the US Fish and Wildlife Service and the Wildlife Management Institute. Any use of trade names is for descriptive purposes only and does not imply endorsement by the US Government. NR 76 TC 1 Z9 1 U1 5 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD JUL PY 2015 VL 7 IS 4 BP 717 EP 726 DI 10.1111/gcbb.12157 PG 10 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CK8ME UT WOS:000356491400014 ER PT J AU Nepal, P Wear, DN Skog, KE AF Nepal, Prakash Wear, David N. Skog, Kenneth E. TI Net change in carbon emissions with increased wood energy use in the United States SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE biogenic carbon accounting; carbon neutrality factor; cumulative radiative forcing; forest carbon; logging slash; harvested wood products; market-induced indirect land-use change; wood energy ID BIOENERGY PRODUCTION; LOGGING RESIDUES; FOSSIL-FUEL; LAND-USE; FOREST; SEQUESTRATION; CONSUMPTION; PRODUCTS; BIOFUELS; MARKETS AB Use of wood biomass for energy results in carbon (C) emissions at the time of burning and alters C stocks on the land because of harvest, regrowth, and changes in land use or management. This study evaluates the potential effects of expanded woody biomass energy use (for heat and power) on net C emissions over time. A scenario with increased wood energy use is compared with a dynamic business-as-usual scenario where wood energy use is driven by its historical relationship with gross domestic product. At the national level, we projected that up to 78% of increased cumulative C emissions from increased wood burning and up to 80% of increased cumulative radiative forcing would be offset over 50years by change in forest area loss, biomass regrowth on land, C storage in harvested wood products, and C in logging slash left in forests. For example, forest area is projected to decline in both scenarios, but 3.5 million hectares more are retained in the high wood energy-use case. Projected C offsets over a 50 year period differed substantially by US region (16% in the North, 50% in the West, and 95% in the South) not only because of differences in forest regrowth and induced investment in retaining and planting forest, but also because of shifts in competitive advantage among regions in producing various wood products. If wood systems displace coal systems that have 75% of the C emissions of wood energy systems per unit energy, then the nationwide net C emissions offset would be reduced to 71-74%. If displacing natural gas systems that have 40% of the level of wood bioenergy emissions per unit energy, the nationwide net C emissions offset would be 46-52%. C1 [Nepal, Prakash] LSU AgCtr, Sch Renewable Nat Resources Bldg, Baton Rouge, LA 70803 USA. [Wear, David N.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Skog, Kenneth E.] US Forest Serv, USDA, Ctr Integrated Forest Sci & Synth, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. RP Nepal, P (reprint author), LSU AgCtr, Sch Renewable Nat Resources Bldg, Baton Rouge, LA 70803 USA. EM pnepal@fs.fed.us FU USDA Forest Service Renewable Resources Planning Act (RPA) assessment FX We acknowledge funding support from the USDA Forest Service Renewable Resources Planning Act (RPA) assessment. Authors are thankful to Dr Peter J. Ince for his advice and planning of this research. NR 35 TC 1 Z9 1 U1 5 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD JUL PY 2015 VL 7 IS 4 BP 820 EP 835 DI 10.1111/gcbb.12193 PG 16 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CK8ME UT WOS:000356491400023 ER PT J AU Mbonimpa, EG Hong, CO Owens, VN Lehman, RM Osborne, SL Schumacher, TE Clay, DE Kumar, S AF Mbonimpa, Eric G. Hong, Chang O. Owens, Vance N. Lehman, R. Michael Osborne, Shannon L. Schumacher, Thomas E. Clay, David E. Kumar, Sandeep TI Nitrogen fertilizer and landscape position impacts on CO2 and CH4 fluxes from a landscape seeded to switchgrass SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE carbon dioxide; landscape positions; methane; nitrogen management; switchgrass ID BIOMASS PRODUCTION; SOIL RESPIRATION; CARBON-DIOXIDE; ATMOSPHERIC METHANE; GREAT-PLAINS; YIELD; TEMPERATURE; BIOENERGY; MANAGEMENT; DIVERSITY AB This study was conducted to evaluate the impacts of N fertilizer and landscape position on carbon dioxide (CO2) and methane (CH4) fluxes from a US Northern Great Plains landscape seeded to switchgrass (Panicum virgatum L.). The experimental design included three N levels (low, 0kgNha(-1); medium, 56kgNha(-1); and high, 112kgNha(-1)) replicated four times. The experiment was repeated at shoulder and footslope positions. Soil CO2 and CH4 fluxes were monitored once every 2weeks from May 2010 to October 2012. The CO2 fluxes were 40% higher at the footslope than the shoulder landscape position, and CH4 fluxes were similar in both landscape positions. Soil CO2 and CH4 fluxes averaged over the sampling dates were not impacted by N rates. Seasonal variations showed highest CO2 release and CH4 uptake in summer and fall, likely due to warmer and moist soil conditions. Higher CH4 release was observed in winter possibly due to increased anaerobic conditions. However, year to year (2010-2012) variations in soil CO2 and CH4 fluxes were more pronounced than the variations due to the impact of landscape positions and N rates. Drought conditions reported in 2012, with higher annual temperature and lower soil moisture than long-term average, resulted in higher summer and fall CO2 fluxes (between 1.3 and 3 times) than in 2011 and 2010. These conditions also promoted a net CH4 uptake in 2012 in comparison to 2010 when there was net CH4 release. Results from this study conclude that landscape positions, air temperature, and soil moisture content strongly influenced soil CO2 fluxes, whereas soil moisture impacted the direction of CH4 fluxes (uptake or release). However, a comprehensive life cycle analysis would be appropriate to evaluate environmental impacts associated with switchgrass production under local environmental conditions. C1 [Mbonimpa, Eric G.; Owens, Vance N.; Schumacher, Thomas E.; Clay, David E.; Kumar, Sandeep] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Hong, Chang O.] Pusan Natl Univ, Busan, South Korea. [Lehman, R. Michael; Osborne, Shannon L.] USDA, Brookings, SD USA. RP Kumar, S (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM sandeep.kumar@sdstate.edu OI Lehman, Michael/0000-0002-3391-3178 FU South Dakota State University (SDSU); North Central Regional Sun Grant Center at SDSU by the US Department of Energy Bioenergy Technologies Office [DE-FC36-05GO85041] FX We thank Mr. Jerry Roitsch for providing the land for the study, Mr. Joe Schumacher and Mr. Seth Owens for assisting in field sampling and collecting weather data. We also thank Dr. Shardendu Singh for his help in Principal Component Analysis. This study was supported by the South Dakota State University (SDSU) and North Central Regional Sun Grant Center at SDSU through a grant provided by the US Department of Energy Bioenergy Technologies Office under award number DE-FC36-05GO85041. NR 46 TC 7 Z9 7 U1 2 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD JUL PY 2015 VL 7 IS 4 BP 836 EP 849 DI 10.1111/gcbb.12187 PG 14 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CK8ME UT WOS:000356491400024 ER PT J AU Liu, W Xu, JN Zhu, R Zhu, YQ Zhao, Y Chen, P Pan, C Yao, WB Gao, XD AF Liu, Wei Xu, Jinnan Zhu, Rui Zhu, Yiqing Zhao, Yang Chen, Pei Pan, Chun Yao, Wenbing Gao, Xiangdong TI Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics SO INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES LA English DT Article DE Lycium barbarum; Fingerprinting profile; Chemometrics ID PERFORMANCE LIQUID-CHROMATOGRAPHY; GYNOSTEMMA-PENTAPHYLLUM; TEA POLYSACCHARIDES; HPLC-DAD; QUANTIFICATION; FRUIT; EXPRESSION; ZEAXANTHIN; SUGARS; CELLS AB Techniques including ultraviolet-visible spectra (UV), high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were used in the fingerprinting analysis of Lycium barbarum polysaccharides (LBPs) from different locations and varieties. Multiple fingerprinting profiles were used to evaluate the similarity and classification of different LBPs with the help of chemometrics. The results indicated that sixteen batches of LBPs had good consistency, and fingerprinting techniques were simple and robust for quality control of LBPs as well as related products. In addition, fingerprinting techniques combined with chemometrics could also be used to identify different cultivation locations of LBPs samples. Finally, four monosaccharides (galacturonic acid, glucose, galactose and arabinose) and the absorptions of stretching vibration of ester carbonyl groups as well as N-H variable angle vibration of-CONH-could be selected as herbal markers to distinguish different samples. (C) 2015 Elsevier B.V. All rights reserved. C1 [Liu, Wei; Xu, Jinnan; Zhu, Rui; Zhu, Yiqing; Yao, Wenbing; Gao, Xiangdong] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Peoples R China. [Liu, Wei; Xu, Jinnan; Zhu, Rui; Zhu, Yiqing; Yao, Wenbing; Gao, Xiangdong] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China. [Zhao, Yang; Chen, Pei] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Pan, Chun] Southeast Univ, Zhongda Hosp, Dept Crit Care Med, Nanjing 210009, Peoples R China. RP Yao, WB (reprint author), China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210009, Peoples R China. EM wbyao@cpu.edu.cn; xdgao@cpu.edu.cn RI ZHAO, YANG/O-7920-2014 FU National Scientific and Technological Major Project for Significant New Drugs Creation [2012ZX09502001-004]; National Natural Science Foundation of China [31301473]; Innovative Scientific Research Team Fund of Jiangsu Province; Priority Academic Program Development of Jiangsu Higher Education Institutions; Graduate Innovation Fund of Huahai Pharmaceutical Company [CX13B-012HH] FX This study was funded by the National Scientific and Technological Major Project for Significant New Drugs Creation (2012ZX09502001-004), the National Natural Science Foundation of China (No. 31301473), Innovative Scientific Research Team Fund of Jiangsu Province, the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Graduate Innovation Fund of Huahai Pharmaceutical Company (CX13B-012HH). NR 43 TC 7 Z9 9 U1 15 U2 78 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0141-8130 EI 1879-0003 J9 INT J BIOL MACROMOL JI Int. J. Biol. Macromol. PD JUL PY 2015 VL 78 BP 230 EP 237 DI 10.1016/j.ijbiomac.2015.03.062 PG 8 WC Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA CK4LN UT WOS:000356195400031 PM 25847838 ER PT J AU Kuhlmann, U Sappington, TW Wang, ZY AF Kuhlmann, Ulrich Sappington, Thomas W. Wang, Zhenying TI Special issue highlighting research presented at the 25th IWGO Conference, Chicago 2014 SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Editorial Material C1 [Kuhlmann, Ulrich] CABI, Delemont, Switzerland. [Sappington, Thomas W.] ARS, USDA, Ames, IA USA. [Wang, Zhenying] Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China. RP Kuhlmann, U (reprint author), CABI, Delemont, Switzerland. EM u.kuhlmann@cabi.org; tom.sappington@ars.usda.gov; zywang@ippcaas.cn NR 0 TC 0 Z9 0 U1 1 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUL PY 2015 VL 139 IS 6 SI SI BP 401 EP 402 DI 10.1111/jen.12243 PG 2 WC Entomology SC Entomology GA CL0EM UT WOS:000356612400001 ER PT J AU Hoffmann, AM French, BW Hellmich, RL Lauter, N Gassmann, AJ AF Hoffmann, A. M. French, B. W. Hellmich, R. L. Lauter, N. Gassmann, A. J. TI Fitness costs of resistance to Cry3Bb1 maize by western corn rootworm SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Bacillus thuringiensis; Diabrotica virgifera virgifera; ecological variation; refuge strategy ID FIELD-EVOLVED RESISTANCE; COLEOPTERA-CHRYSOMELIDAE; BACILLUS-THURINGIENSIS; HOST-PLANT; BT MAIZE; INSECT RESISTANCE; TRANSGENIC MAIZE; MANAGEMENT; ADAPTATION; SELECTION AB Crops producing toxins derived from the bacterium Bacillus thuringiensis (Bt) are widely planted to manage insect pests including western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), which is a significant pest of maize in the United States and Europe. However, the widespread use of Bt maize places intense selection pressure on pest populations to evolve resistance, and field-evolved resistance to Bt maize by western corn rootworm has been documented in the United States. In conjunction with non-Bt refuges, fitness costs of Bt resistance can delay resistance evolution. Fitness costs arise in the absence of Bt toxin when individuals with resistance alleles have lower fitness than Bt-susceptible genotypes. We quantified the level of resistance and fitness costs of resistance for a strain of western corn rootworm with laboratory-selected resistance to transgenic maize (Zea mays L.) producing Bt toxin Cry3Bb1. Survival to adulthood on Cry3Bb1 maize was more than twofold higher for resistant insects vs. susceptible insects, which is similar to the magnitude of resistance first observed in the field. Fitness costs were measured in two experiments; the first used maize hybrids and the second used inbred lines. The experiment with maize hybrids compared resistant and susceptible strains while the experiment with maize inbreds compared resistant, susceptible and heterozygous genotypes. The only non-recessive fitness cost detected (i.e. cost affecting heterozygotes) was for adult size. Recessive fitness costs (i.e. costs affecting the resistant strain) were observed for developmental rate, female survival and egg viability. However, when reared on non-Bt maize, the resistant strain also displayed higher fecundity, higher survival for males and greater adult longevity compared to the susceptible strain. These results suggest that resistance to Bt maize by western corn rootworm may not impose substantial fitness costs, and consequently, may evolve quickly and persist once present. C1 [Hoffmann, A. M.; Gassmann, A. J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [French, B. W.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD USA. [Hellmich, R. L.; Lauter, N.] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. RP Gassmann, AJ (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM aaronjg@iastate.edu FU United States Department of Agriculture [2009-33120-20256] FX Sean Bradley and Brandon Sorgatz assisted with experiments. Funding was provided by the United States Department of Agriculture through a Biotechnology Risk Assessment Grant 2009-33120-20256. Mention of a proprietary product does not constitute an endorsement or a recommendation by Iowa State University or USDA for its use. NR 45 TC 6 Z9 7 U1 1 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUL PY 2015 VL 139 IS 6 SI SI BP 403 EP 415 DI 10.1111/jen.12209 PG 13 WC Entomology SC Entomology GA CL0EM UT WOS:000356612400002 ER PT J AU Lundgren, JG McDonald, T Rand, TA Fausti, SW AF Lundgren, J. G. McDonald, T. Rand, T. A. Fausti, S. W. TI Spatial and numerical relationships of arthropod communities associated with key pests of maize SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE aphids; community; Diabrotica spp; Ostrinia nubilalis; Striacosta albicosta; thrips ID EUROPEAN CORN-BORER; IN-FIELD CORN; CHRYSOMELIDAE)-PROTECTED BT CORN; BACILLUS-THURINGIENSIS CORN; ALBICOSTA SMITH LEPIDOPTERA; ORIUS-INSIDIOSUS HEMIPTERA; WESTERN BEAN CUTWORM; NATURAL ENEMIES; LEAF APHID; COLEOMEGILLA-MACULATA AB Pest management largely focuses on managing individual pest species with little concern for the diverse communities that co-occur with key pests and potentially shape their population dynamics. During anthesis, we described the foliar arthropod communities on 53 maize farms throughout the region of eastern South Dakota. The resulting communities were examined for trends in local associations in the abundances of taxa with key pests in the system (rootworms [Diabrotica spp.], European corn borers [Ostrinia nubilalis], aphids and Western bean cutworm [Striacosta albicosta]) using regression analyses. Regional spatial clustering in the abundances of key pests with members of the community was explored using Moran's I test statistic. The distributions of rootworms and European corn borer were mapped. A total of 37185 arthropods representing at least 91 taxa were collected in South Dakota maize; there was an average of 5.06 predators and 8.29 herbivores found per plant. Key pests were never found at economically threatening levels (with one exception for Diabrotica). Numerous species were consistently numerically associated with each of the key pests across the farms during anthesis. Occasionally, these pests shared species with which they were locally associated with; for example, coccinellid egg abundances were predictive of the abundances of all key pest species except rootworm adults. Spatial analysis across the region suggested that species co-occurred with key pests based on local characteristics surrounding the fields, rather than as a result of regional characteristics. Exceptions were documented; namely aphids and Western bean cutworms that spatially clustered with a handful of other members of the community. The results of the study point out that the abundances of key pests of maize were interconnected through indirect associations in the abundances of other members of the community. These associations may be useful for manipulating maize agroecosystems to minimize the effects of maize pests. C1 [Lundgren, J. G.] ARS, USDA, NCARL, Brookings, SD 57006 USA. [McDonald, T.] Ohio Univ, Dept Econ, Athens, OH USA. [Rand, T. A.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT USA. [Fausti, S. W.] S Dakota State Univ, Dept Econ, Brookings, SD 57007 USA. RP Lundgren, JG (reprint author), ARS, USDA, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM Jonathan.Lundgren@ars.usda.gov FU USDA-AFRI [2010-85605-20552] FX Janet Fergen, Emmanuel Opuku, Mike Bredeson, Chloe Kruse, Ryan Schmid, Ashton Walter helped gather and collate the arthropod collections for this study. We thank Dr. Steven Holland for free use of Analytic Rarefaction 1.3, which is available on the website http://strata.uga.edu/software/anRareReadme.html; Mark Longfellow assisted in applying this analysis. The interpretation of the data presented in this manuscript does not represent an official position by the USDA. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. This research was funded by USDA-AFRI award 2010-85605-20552. NR 64 TC 1 Z9 1 U1 5 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUL PY 2015 VL 139 IS 6 SI SI BP 446 EP 456 DI 10.1111/jen.12215 PG 11 WC Entomology SC Entomology GA CL0EM UT WOS:000356612400006 ER PT J AU Ma, R Zhang, TC Bartelt-Hunt, SL Qi, Y Kranz, WL Snow, DD Mader, TL Shapiro, CA Shelton, DP van Donk, SJ Tarkalson, DD Ensley, S AF Ma, Rui Zhang, Tian C. Bartelt-Hunt, Shannon L. Qi, Yong Kranz, William L. Snow, Daniel D. Mader, Terry L. Shapiro, Charles A. Shelton, David P. van Donk, Simon J. Tarkalson, David D. Ensley, Steve TI Influence of Soil Properties and Test Conditions on Sorption and Desorption of Testosterone SO JOURNAL OF ENVIRONMENTAL ENGINEERING LA English DT Article DE Sorption; Desorption; Hormone; Testosterone; Soil; Fate and transport ID WASTE-WATER CONTAMINANTS; STEROID-HORMONES; AGRICULTURAL SOILS; ORGANIC-COMPOUNDS; TRANSPORT; 17-BETA-ESTRADIOL; FATE; SEDIMENTS; MANURE; ESTROGENS AB In this study, batch sorption and desorption experiments were conducted for testosterone using four agricultural soils and five clay minerals. Significant differences in sorption behavior were observed between abiotic and biotic systems. The Freundlich sorption coefficient K-f (mu g/g) / (mu g/mL)(n) ranged from 8.53 to 74.46 for soils and from 35.28 to 1,243 for clays. The maximum sorption capacity (mu g/g) of soils ranged from 25.25 to 440.61 for soils and from 168.46 to 499.84 for clays. Correlation of the sorption model parameters with the soil properties indicated that both clay content and soil organic matter are important variables in predicting testosterone sorption behavior. Observed testosterone desorption from agricultural soils ranged from approximately 14 to 100% after three desorption cycles, and the desorption percentage decreased as the initial testosterone concentration decreased. It was determined that the temperature, ionic strength, water/soil ratio, and soil depth influenced the sorption and desorption of testosterone. Desorption significantly increased with the soil depth (p < 0.05) and with the increase in the water/soil ratio. Temperature had an inverse effect on the sorption capacity of the soils tested. Thermodynamic calculations showed that the enthalpy change (Delta H-0) of the soils tested ranged from 12.9 to 20.7 kJ/mol, indicating a weak interaction between the testosterone and soil. The authors' results suggest that additional studies on how soil particles with different size fractions affect hormones' fate and transport are needed to determine the potential risk of testosterone leaching or runoff. (C) 2015 American Society of Civil Engineers. C1 [Ma, Rui] Chinese Acad Environm Planning, Minist Environm Protect, Ecol Environm Inst, Beijing 100012, Peoples R China. [Zhang, Tian C.; Bartelt-Hunt, Shannon L.; Qi, Yong] Univ Nebraska Lincoln, Dept Civil Engn, Omaha, NE 68182 USA. [Kranz, William L.; Mader, Terry L.; Shapiro, Charles A.; Shelton, David P.] Univ Nebraska, Haskell Agr Lab, Concord, NE 68728 USA. [Snow, Daniel D.] Univ Nebraska, Water Sci Lab, Lincoln, NE 68583 USA. [van Donk, Simon J.] Univ Nebraska Lincoln, West Cent Res & Extens Ctr, North Platte, NE 69101 USA. [Tarkalson, David D.] USDA ARS, NW Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Ensley, Steve] Iowa State Univ, Coll Vet Med, Ames, IA 50011 USA. RP Zhang, TC (reprint author), Univ Nebraska Lincoln, Dept Civil Engn, Omaha Campus, Omaha, NE 68182 USA. EM tzhang1@unl.edu FU USEPA Science to Achieve Results (STAR) program [EPA-G2006-STAR-M1]; National Science Foundation [1067537] FX The authors thank Dr. Mitiku Mamo for his help with field sampling and Dr. James A. Smith at the University of Virginia for soil surface area tests. This research was made possible through support from the USEPA Science to Achieve Results (STAR) program (EPA-G2006-STAR-M1) and the National Science Foundation (Award ID: 1067537). NR 35 TC 4 Z9 4 U1 3 U2 21 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9372 EI 1943-7870 J9 J ENVIRON ENG JI J. Environ. Eng.-ASCE PD JUL PY 2015 VL 141 IS 7 AR 04015006 DI 10.1061/(ASCE)EE.1943-7870.0000937 PG 9 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CK7EX UT WOS:000356395500008 ER PT J AU Rigsby, CM Showalter, DN Herms, DA Koch, JL Bonello, P Cipollini, D AF Rigsby, C. M. Showalter, D. N. Herms, D. A. Koch, J. L. Bonello, P. Cipollini, D. TI Physiological responses of emerald ash borer larvae to feeding on different ash species reveal putative resistance mechanisms and insect counter-adaptations SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Adaptations to host defense; Wood-boring insects; Oxidative stress; Antioxidant enzymes; Detoxification Host plant resistance ID EUCALYPTUS LONGHORNED BORER; ANOPLOPHORA-GLABRIPENNIS; PROTEASE INHIBITORS; MONOAMINE-OXIDASE; PHLOEM PHENOLICS; HELICOVERPA-ZEA; PROTEINS; DEFENSE; ENZYMES; ALLELOCHEMICALS AB Emerald ash borer, Agrilus planipennis Fairmaire, an Asian wood-boring beetle, has devastated ash (Fraxinus spp.) trees in North American forests and landscapes since its discovery there in 2002. In this study, we collected living larvae from EAB-resistant Manchurian ash (Fraxinus mandschurica), and susceptible white (Fraxinus americana) and green (Fraxinus pennsylvanica) ash hosts, and quantified the activity and production of selected detoxification, digestive, and antioxidant enzymes. We hypothesized that differences in larval physiology could be used to infer resistance mechanisms of ash. We found no differences in cytochrome P450, glutathione-S-transferase, carboxylesterase, sulfotransferase, and typtic BApNAase activities between larvae feeding on different hosts. Despite this, Manchurian ash-fed larvae produced a single isozyme of low electrophoretic mobility that was not produced in white or green ash-fed larvae. Additionally, larvae feeding on white and green ash produced two serine protease isozymes of high electrophoretic mobility that were not observed in Manchurian ash-fed larvae. We also found lower activity of B-glucosidase and higher activities of monoamine oxidase, ortho-quinone reductase, catalase, superoxide dismutase, and glutathione reductase in Manchurian ash-fed larvae compared to larvae that had fed on susceptible ash. A single isozyme was detected for both catalase and superoxide dismutase in all larval groups. The activities of the quinone-protective and antioxidant enzymes are consistent with the resistance phenotype of the host species, with the highest activities measured in larvae feeding on resistant Manchurian ash. We conclude that larvae feeding on Manchurian ash could be under quinone and oxidative stress, suggesting these may be potential mechanisms of resistance of Manchurian ash to EAB larvae, and that quinone-protective and antioxidant enzymes are important counter-adaptations of larvae for dealing with these resistance mechanisms. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Rigsby, C. M.; Cipollini, D.] Wright State Univ, Dept Biol Sci & Environm Sci, PhD Program, Dayton, OH 45435 USA. [Showalter, D. N.; Bonello, P.] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. [Herms, D. A.] Ohio State Univ, Dept Entomol, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Koch, J. L.] US Forest Serv, No Res Stn, USDA, Delaware, OH 43015 USA. RP Rigsby, CM (reprint author), Wright State Univ, Dept Biol Sci & Environm Sci, PhD Program, 3640 Colonel Glenn Highway, Dayton, OH 45435 USA. RI Showalter, David/D-4142-2013 OI Showalter, David/0000-0001-9853-4905 FU USDA APHIS Accelerated Emerald Ash Borer Research Program; Ohio Agricultural Research and Development Center, The Ohio State University; Wright State University FX We thank Dr. Michael Leffak and Joanna Bartholomew (Wright State University) for use of their gel imager. Jonathan Lelito (USDA-APHIS) and Therese Poland (Michigan State University) provided EAB eggs. Robert Hansen, Alejandro Chiriboga, Amilcar Vargas, Diane Hartzler, Paul Snyder, Amy Hill, Anna Conrad, Patrick Sherwood, Caterina Villari, Mark Miller, Mary Mason (all from The Ohio State University), and David Carey (USDA Forest Service Northern Research Station) assisted with maintaining ash trees and maintaining and collecting larvae. This project was funded by the USDA APHIS Accelerated Emerald Ash Borer Research Program, by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, and Wright State University. NR 60 TC 5 Z9 5 U1 7 U2 61 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD JUL PY 2015 VL 78 BP 47 EP 54 DI 10.1016/j.jinsphys.2015.05.001 PG 8 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA CK9EZ UT WOS:000356544700006 PM 25956198 ER PT J AU Claycombe, KJ Vomhof-DeKrey, EE Roemmich, JN Rhen, T Ghribi, O AF Claycombe, Kate J. Vomhof-DeKrey, Emilie E. Roemmich, James N. Rhen, Turk Ghribi, Othman TI Maternal low-protein diet causes body weight loss in male, neonate Sprague-Dawley rats involving UCP-1-mediated thermogenesis SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Maternal low protein diet; Brown fat thermogenesis; Irisin; PRDM16; UCP-1; Placental irisin; FNDC5 ID PITUITARY TSH RESPONSE; BROWN ADIPOSE-TISSUE; ADULT RATS; RESTRICTION; LACTATION; HYPERTENSION; EXPRESSION; MALNUTRITION; GESTATION; PREGNANCY AB Brown adipose tissue (BAT) plays an important role in regulating body weight (BW) by modifying thermogenesis. Maternal low protein (LP) diets reduce offspring birth weight. Increased BAT thermogenesis in utero may be one mechanism for the lower BW. However, whether maternal LP nutrition alters BAT thermogenesis and BW of offspring in utero is not yet known. We fed obese-prone Sprague-Dawley dams 8% LP or 20% normal protein (NP) diets for 3 weeks prior to breeding and through pregnancy. BW and gene expression of interscapular BAT (iBAT) thermogenic markers were measured in male fetal (gestation day 18) and neonatal (day 0 or 1) offspring. BW of neonatal LP males was lower than NP males but no difference was observed in females. Gene and protein expression of UCP-1 and transcription factors PRDM16 and PPAR alpha in iBAT were 2- to 6-fold greater in LP than in NP male neonatal offspring. FNDC5, a precursor of irisin and activator of thermogenesis, was expressed 2-fold greater in neonatal LP iBAT than NP males. However, fetal iBAT UCP-1, PRDM16, PPAR alpha and irisin mRNA did not differ between LP and NP groups. Maternal LP diet had no effects on placental irisin and UCP-2 expression. These results suggest that prenatal protein restriction increases the risk for low BW through mechanisms affecting full-term offspring iBAT thermogenesis but not greatly altering fetal iBAT or placental thermogenesis. Published by Elsevier Inc. C1 [Claycombe, Kate J.; Vomhof-DeKrey, Emilie E.; Roemmich, James N.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Rhen, Turk] Univ N Dakota, Dept Biol Sci, Grand Forks, ND 58202 USA. [Ghribi, Othman] Univ N Dakota, Dept Pharmacol Physiol & Therapeut, Sch Med & Hlth Sci, Grand Forks, ND 58202 USA. RP Claycombe, KJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 Second Ave North, Grand Forks, ND 58203 USA. EM kate.claycombe@ars.usda.gov FU U.S. Department of Agriculture Agricultural Research Service Project [5450-51000-047-00D] FX This work was supported by U.S. Department of Agriculture Agricultural Research Service Project #5450-51000-047-00D. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 28 TC 5 Z9 6 U1 2 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD JUL PY 2015 VL 26 IS 7 BP 729 EP 735 DI 10.1016/j.jnutbio.2015.01.008 PG 7 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA CK9LM UT WOS:000356561600006 PM 25858881 ER PT J AU MacDonald, RL Chen, HYH Bartels, SF Palik, BJ Prepas, EE AF MacDonald, Rebecca L. Chen, Han Y. H. Bartels, Samuel F. Palik, Brian J. Prepas, Ellie E. TI Compositional stability of boreal understorey vegetation after overstorey harvesting across a riparian ecotone SO JOURNAL OF VEGETATION SCIENCE LA English DT Article DE boreal forest; disturbance; diversity-stability hypothesis; riparian vegetation; succession; understorey plants ID PLANT-COMMUNITIES; FOREST; DIVERSITY; BIODIVERSITY; CONSEQUENCES; ASSEMBLAGES; DYNAMICS; DETERMINANTS; RESILIENCE; LANDSCAPE AB QuestionsUnderstanding factors that contribute to the stability of an ecosystem following harvesting is central to predicting responses of boreal ecosystems to increasing human disturbances. While the response of understorey vegetation to harvesting is well understood for upland sites, little is known about compositional stability of riparian understorey vegetation. We examined how compositional stability changes with or without harvesting along an upland to streamside gradient and tested whether compositional stability is affected by pre-harvest species diversity and composition. LocationLower Foothills sub-region of the Boreal Plain, ca. 20km northwest of Whitecourt, Alberta, CA. MethodsWe repeatedly sampled understorey vegetation of four winter-harvested and four unharvested sites in western Canadian boreal forest. Species covers were measured during the summer prior to harvesting in 2003 (year 0) and in 2004, 2008 and 2010 (1, 5 and 7yrs after, respectively). We used non-metric multidimensional scaling to ordinate plots using species covers, and measured vector length in the ordination space to quantify floristic dissimilarity of each plot between year 0 and years 1, 5 and 7, an inverse measure of compositional stability. ResultsFloristic dissimilarity between year 0 and subsequent years was significantly greater in harvested than unharvested sites for all subsequent sampling years. Furthermore, along the upland to stream gradient, floristic dissimilarity was larger on upland than stream sites. Additional analyses revealed that floristic dissimilarity was related negatively to pre-harvesting species richness and evenness, but positively to bryophyte cover. ConclusionsOur results demonstrate that understorey compositional stability is strongly influenced by harvesting, and streamside communities are more stable than upland communities. Our results indicate that compositional stability of understorey vegetation in response to harvesting is associated positively with pre-disturbance species richness and evenness, but negatively with bryophyte dominance. C1 [MacDonald, Rebecca L.; Chen, Han Y. H.; Bartels, Samuel F.; Prepas, Ellie E.] Lakehead Univ, Fac Nat Resources Management, Thunder Bay, ON P7B 5E1, Canada. [Palik, Brian J.] US Forest Serv, Ctr Res Ecosyst Change, No Res Stn, USDA, Grand Rapids, MN 55744 USA. RP Chen, HYH (reprint author), Lakehead Univ, Fac Nat Resources Management, Thunder Bay, ON P7B 5E1, Canada. EM rmacdon2@lakeheadu.ca; hchen1@lakeheadu.ca; sfbartel@lakeheadu.ca; bpalik@fs.fed.us; ellie.prepas@lakeheadu.ca RI Chen, Han/A-1359-2008; OI Chen, Han/0000-0001-9477-5541; Bartels, Samuel/0000-0003-2676-3295 FU Natural Sciences and Engineering Research Council of Canada (NSERC) Industrial (Millar Western Forest Products Ltd.) Post-graduate Scholarship; NSERC FX We would like to thank S. Luke, T. McCready and M. Serediak for help in initiating this study; J. Burke for providing valuable comments on an earlier version of the manuscript; and S. Eberwein, T. Butler, L. Salmon and M. LeRoux for assistance in the field. Funding for RLM was provided by a Natural Sciences and Engineering Research Council of Canada (NSERC) Industrial (Millar Western Forest Products Ltd.) Post-graduate Scholarship. This study is part of the NSERC-funded CRD project FORWARD and partners (http://forward.lakeheadu.ca/). NR 38 TC 1 Z9 1 U1 5 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1100-9233 EI 1654-1103 J9 J VEG SCI JI J. Veg. Sci. PD JUL PY 2015 VL 26 IS 4 BP 733 EP 741 DI 10.1111/jvs.12272 PG 9 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CL2XO UT WOS:000356811300013 ER PT J AU Lonsinger, RC Gese, EM Dempsey, SJ Kluever, BM Johnson, TR Waits, LP AF Lonsinger, Robert C. Gese, Eric M. Dempsey, Steven J. Kluever, Bryan M. Johnson, Timothy R. Waits, Lisette P. TI Balancing sample accumulation and DNA degradation rates to optimize noninvasive genetic sampling of sympatric carnivores SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE Canis latrans; DNA degradation; genotyping error; noninvasive genetic sampling; scat deposition; Vulpes macrotis ID GREAT-BASIN DESERT; FECAL DNA; URSUS-ARCTOS; GENOTYPING ERRORS; POPULATION-SIZE; FECES; IDENTIFICATION; PRESERVATION; DIET; AMPLIFICATION AB Noninvasive genetic sampling, or noninvasive DNA sampling (NDS), can be an effective monitoring approach for elusive, wide-ranging species at low densities. However, few studies have attempted to maximize sampling efficiency. We present a model for combining sample accumulation and DNA degradation to identify the most efficient (i.e. minimal cost per successful sample) NDS temporal design for capture-recapture analyses. We use scat accumulation and faecal DNA degradation rates for two sympatric carnivores, kit fox (Vulpes macrotis) and coyote (Canis latrans) across two seasons (summer and winter) in Utah, USA, to demonstrate implementation of this approach. We estimated scat accumulation rates by clearing and surveying transects for scats. We evaluated mitochondrial (mtDNA) and nuclear (nDNA) DNA amplification success for faecal DNA samples under natural field conditions for 20 fresh scats/species/season from <1-112days. Mean accumulation rates were nearly three times greater for coyotes (0.076scats/km/day) than foxes (0.029scats/km/day) across seasons. Across species and seasons, mtDNA amplification success was 95% through day 21. Fox nDNA amplification success was 70% through day 21 across seasons. Coyote nDNA success was 70% through day 21 in winter, but declined to <50% by day 7 in summer. We identified a common temporal sampling frame of approximately 14days that allowed species to be monitored simultaneously, further reducing time, survey effort and costs. Our results suggest that when conducting repeated surveys for capture-recapture analyses, overall cost-efficiency for NDS may be improved with a temporal design that balances field and laboratory costs along with deposition and degradation rates. C1 [Lonsinger, Robert C.; Waits, Lisette P.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Gese, Eric M.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Logan, UT 84322 USA. [Gese, Eric M.; Dempsey, Steven J.; Kluever, Bryan M.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Johnson, Timothy R.] Univ Idaho, Dept Stat Sci, Moscow, ID 83844 USA. RP Lonsinger, RC (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, 875 Perimeter Dr MS1136, Moscow, ID 83844 USA. EM Lons1663@vandals.uidaho.edu RI Gese, Eric/B-4578-2011 FU U.S. Department of Defense Environmental Security Technology Certification Programme [12-EB-RC5-006]; Legacy Resource Management Programme [W9132T-12-2-0050]; Army DPG Environmental Programme; U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center; Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources FX Funding provided by the U.S. Department of Defense Environmental Security Technology Certification Programme (12-EB-RC5-006), Legacy Resource Management Programme (W9132T-12-2-0050) and Army DPG Environmental Programme. Additional funding and logistical assistance provided by the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center; and the Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources. R Knight was essential to securing funding and provided logistical support. J Adams assisted with laboratory procedures. NR 46 TC 12 Z9 12 U1 5 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X EI 1755-0998 J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD JUL PY 2015 VL 15 IS 4 BP 831 EP 842 DI 10.1111/1755-0998.12356 PG 12 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CL1CJ UT WOS:000356679800013 PM 25454561 ER PT J AU Wolfe, JD Ralph, CJ Elizondo, P AF Wolfe, Jared D. Ralph, C. John Elizondo, Pablo TI Changes in the apparent survival of a tropical bird in response to the El Nio Southern Oscillation in mature and young forest in Costa Rica SO OECOLOGIA LA English DT Article DE El Nino Southern Oscillation; ENSO; Frugivore; SOI; Tropical bird ID CLIMATE-CHANGE; AMAZON BASIN; PATTERNS; FRAGMENTATION; MOVEMENT; DYNAMICS; RAINFALL; AMERICA; IMPACTS; SYSTEM AB The effects of habitat alteration and climatic instability have resulted in the loss of bird populations throughout the globe. Tropical birds in particular may be sensitive to climate and habitat change because of their niche specialization, often sedentary nature, and unique life-cycle phenologies. Despite the potential influence of habitat and climatic interactions on tropical birds, we lack comparisons of avian demographics from variably aged forests subject to different climatic phenomena. Here, we measured relationships between forest type and climatic perturbations on White-collared Manakin (Manacus candei), a frugivorous tropical bird, by using 12 years of capture data in young and mature forests in northeastern Costa Rica. We used Cormack-Jolly-Seber models and an analysis of deviance to contrast the influence of the El Nio Southern Oscillation (ENSO) on manakin survival. We found that ENSO had little effect on manakin survival in mature forests. Conversely, in young forests, ENSO explained 79 % of the variation where dry El Nio events negatively influenced manikin survival. We believe mature forest mitigated negative effects of dry El Nio periods and can serve as refugia for some species by buffering birds from climatic instability. Our results represent the first published documentation that ENSO influences the survival of a resident Neotropic landbird. C1 [Wolfe, Jared D.; Ralph, C. John] USDA Forest Serv, Pacific Southwest Res Stn, Arcata, CA USA. [Wolfe, Jared D.; Ralph, C. John] Klamath Bird Observ, Ashland, OR 97520 USA. [Elizondo, Pablo] Costa Rica Bird Observ, Inst Nacl Biodiversidad INBio, Santo Domingo De Heredia, Costa Rica. RP Wolfe, JD (reprint author), Klamath Bird Observ, Ashland, OR 97520 USA. EM jdw@klamathbird.org RI Dey, Kamalesh/E-6568-2017 NR 36 TC 6 Z9 6 U1 5 U2 31 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD JUL PY 2015 VL 178 IS 3 BP 715 EP 721 DI 10.1007/s00442-015-3256-z PG 7 WC Ecology SC Environmental Sciences & Ecology GA CK9BH UT WOS:000356534400008 PM 25687831 ER PT J AU Yang, JY Cushman, SA Song, XM Yang, J Zhang, PJ AF Yang, Jiuyan Cushman, Samuel A. Song, Xuemei Yang, Jie Zhang, Pujin TI Genetic diversity and drivers of genetic differentiation of Reaumuria soongorica of the Inner Mongolia plateau in China SO PLANT ECOLOGY LA English DT Article DE Reaumuria soongorica; Reciprocal causal modeling; Landscape genetics; Climate; STRUCTURE analysis ID MULTILOCUS GENOTYPE DATA; LANDSCAPE GENETICS; MANTEL TEST; FLOW; POPULATION; SPECIATION; INFERENCE; AMPLIFICATION; DIVERGENCE; REGRESSION AB We quantified genetic diversity and gene flow among eight populations of Reaumuria soongorica in Inner Mongolia, China. Our results showed that genetic differentiation of R. soongorica across the Inner Mongolian plateau is primarily clinal in nature and is driven primarily by differential landscape resistance across areas with changing patterns of seasonal precipitation, perhaps as a result of differential timing of reproductive phenology along precipitation gradients. Finding that seasonal patterns of precipitation, and not temperature, drive population connectivity and gene flow may have important implications for predicting the effects of climate change on this keystone foundation species and devising effective strategies to utilize it in restoration efforts to ameliorate ongoing desertification in the region. Genetic diversity was highest in the western part of the sampled population, perhaps indicating that this region has historically harbored the highest effective population size of the species or may have served as the source of recent range expansion to other parts of the sampled range which exhibited lower genetic diversity. Understanding the ecological drivers of these relationships might be critical to resolving the causes of the geographical pattern of diversity, and could be important in understanding the ecology of the species sufficiently to anticipate climate change effects and effectively implement management strategies to restore the species and combat desertification. C1 [Yang, Jiuyan; Song, Xuemei; Yang, Jie] Inner Mongolia Univ, Sch Life Sci, Hohhot 010021, Peoples R China. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Zhang, Pujin] Inner Mongolia Acad Agr & Anim Husb Sci, Chinese Acad Sci, Inner Mongolia Prataculture Res, Hohhot 010031, Peoples R China. RP Cushman, SA (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. EM scushman@fs.fed.us FU State Key Basic Research Development Programme of China [2012CB722201]; science and technology research projects of Inner Mongolia Education Department of China [NJ10002]; Natural Science Foundation of Inner Mongolia Autonomous Region of China [2014MS0350]; US Forest Service Rocky Mountain Research Station FX This work was supported by The State Key Basic Research Development Programme of China (Grant No. 2012CB722201), science and technology research projects of Inner Mongolia Education Department of China (NJ10002), The Natural Science Foundation of Inner Mongolia Autonomous Region of China (2014MS0350), and US Forest Service Rocky Mountain Research Station. NR 67 TC 1 Z9 1 U1 4 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD JUL PY 2015 VL 216 IS 7 BP 925 EP 937 DI 10.1007/s11258-015-0479-3 PG 13 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CK6XT UT WOS:000356372800002 ER PT J AU Garcia-Plazaola, JI Fernandez-Marin, B Duke, SO Hernandeza, A Lopez-Arbeloa, F Becerril, JM AF Ignacio Garcia-Plazaola, Jose Fernandez-Marin, Beatriz Duke, Stephen O. Hernandeza, Antonio Lopez-Arbeloa, Fernando Maria Becerril, Jose TI Autofluorescence: Biological functions and technical applications SO PLANT SCIENCE LA English DT Review DE Biocommunication; Emission; Fluorescence; Fluorophore; Green fluorescent protein ID GREEN FLUORESCENT PROTEIN; CHLOROPHYLL FLUORESCENCE; FERULIC ACID; GRAPEVINE LEAVES; PLANTS; LOCALIZATION; SPECTROSCOPY; EMISSION; TISSUE; PHOTOINHIBITION AB Chlorophylls are the most remarkable examples of fluorophores, and their fluorescence has been intensively studied as a non-invasive tool for assessment of photosynthesis. Many other fluorophores occur in plants, such as alkaloids, phenolic compounds and porphyrins. Fluorescence could be more than just a physicochemical curiosity in the plant kingdom, as several functional roles in biocommunication occur or have been proposed. Besides, fluorescence emitted by secondary metabolites can convert damaging blue and UV into wavelengths potentially useful for photosynthesis. Detection of the fluorescence of some secondary phytochemicals may be a cue for some pollinators and/or seed dispersal organisms. Independently of their functions, plant fluorophores provide researchers with a tool that allows the visualization of some metabolites in plants and cells, complementing and overcoming some of the limitations of the use of fluorescent proteins and dyes to probe plant physiology and biochemistry. Some fluorophores are influenced by environmental interactions, allowing fluorescence to be also used as a specific stress indicator. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Ignacio Garcia-Plazaola, Jose; Fernandez-Marin, Beatriz; Hernandeza, Antonio; Maria Becerril, Jose] Univ Basque Country, UPV EHU, Dept Biol Vegetal & Ecol, E-48080 Bilbao, Spain. [Fernandez-Marin, Beatriz] Univ Innsbruck, Inst Bot, A-6020 Innsbruck, Austria. [Fernandez-Marin, Beatriz] Univ Innsbruck, Ctr Mol Biosci Innsbruck, A-6020 Innsbruck, Austria. [Duke, Stephen O.] Univ Mississippi, ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Lopez-Arbeloa, Fernando] Univ Basque Country, UPV EHU, Dept Quim Fis, E-48080 Bilbao, Spain. RP Garcia-Plazaola, JI (reprint author), Univ Basque Country, UPV EHU, Dept Biol Vegetal & Ecol, Apdo 644, E-48080 Bilbao, Spain. EM joseignacio.garcia@ehu.es RI HERNANDEZ, ANTONIO/L-1702-2014; Fernandez-Marin, Beatriz/D-6701-2016 OI HERNANDEZ, ANTONIO/0000-0002-8718-7685; Fernandez-Marin, Beatriz/0000-0001-9951-0489 FU Feder [BFU 2010-15021]; Spanish Ministry of Economy (Mineco) [AGL 2012-3915-CO3-01]; Basque Government [UPV/EHU-GV IT-299-07]; Marie Curie IEF grant from the European FP7-PEOPLE [328370 MELISSA] FX The authors acknowledge the support of research grants BFU 2010-15021 (co-funded by Feder) and AGL 2012-3915-CO3-01 from the Spanish Ministry of Economy (Mineco), UPV/EHU-GV IT-299-07 from the Basque Government and Marie Curie IEF grant (328370 MELISSA) from the European FP7-PEOPLE received by BFM. NR 61 TC 3 Z9 3 U1 12 U2 76 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD JUL PY 2015 VL 236 BP 136 EP 145 DI 10.1016/j.plantsci.2015.03.010 PG 10 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CK9JO UT WOS:000356556600012 PM 26025527 ER PT J AU Yin, H Sumners, LH Dalloul, RA Miska, KB Fetterer, RH Jenkins, MC Zhu, Q Wong, EA AF Yin, H. Sumners, L. H. Dalloul, R. A. Miska, K. B. Fetterer, R. H. Jenkins, M. C. Zhu, Q. Wong, E. A. TI Changes in expression of an antimicrobial peptide, digestive enzymes, and nutrient transporters in the intestine of E. praecox-infected chickens SO POULTRY SCIENCE LA English DT Article DE Eimeria praecox; nutrient transporter; liver expressed antimicrobial peptide 2 ID CAMPYLOBACTER-JEJUNI; EIMERIA-ACERVULINA; BROILERS; FAMILY AB Coccidiosis is a major intestinal disease of poultry, caused by several species of the protozoan Eimeria. The objective of this study was to examine changes in expression of digestive enzymes, nutrient transporters, and an antimicrobial peptide following an Eimeria praecox challenge of chickens at days 3 and 6 post-infection. Gene expression was determined by real-time PCR and analyzed by one-way ANOVA. In the duodenum, the primary site of E. praecox infection, a number of genes were downregulated at both d3 and d6 post-infection. These genes included liver ex-pressed antimicrobial peptide 2 (LEAP2), the cationic (CAT1), anionic (EAAT3), and L-type (LAT1) amino acid transporters, the peptide transporter PepT1 and the zinc transporter ZnT1. Other transporters were downregulated either at d3 or d6. At both d3 and d6, there was downregulation of BoAT and CAT1 in the jejunum and downregulation of LEAP2 and LAT1 in the ileum. LEAP2, EAAT3, and ZnT1 have been found to be downregulated following challenge with other Eimeria species, suggesting a common cellular response to Eimeria. C1 [Yin, H.; Zhu, Q.] Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key, Chengdu 611130, Sichuan, Peoples R China. [Yin, H.; Sumners, L. H.; Dalloul, R. A.; Wong, E. A.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. [Miska, K. B.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Fetterer, R. H.; Jenkins, M. C.] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Wong, EA (reprint author), Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. EM ewong@vt.edu OI Dalloul, Rami/0000-0003-4690-9220 FU China Scholarship Council (Beijing) FX HY was supported by a grant from the China Scholarship Council (Beijing). NR 19 TC 2 Z9 2 U1 1 U2 20 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUL PY 2015 VL 94 IS 7 BP 1521 EP 1526 DI 10.3382/ps/pev133 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CK9BX UT WOS:000356536500012 PM 26015586 ER PT J AU Ehr, IJ Kerr, BJ Persia, ME AF Ehr, I. J. Kerr, B. J. Persia, M. E. TI Effects of peroxidized corn oil on performance, AMEn, and abdominal fat pad weight in broiler chicks SO POULTRY SCIENCE LA English DT Article DE broiler; energy; lipid peroxidation; performance ID DISTILLERS DRIED GRAINS; LIPID OXIDATION; SOYBEAN OIL; PEROXIDATION; SOLUBLES AB There is a trend to use more alternative lipids in poultry diets, either through animal-vegetable blends, distillers corn oil, or yellow grease. This has resulted in the use of lipids in poultry diets with a higher concentration of unsaturated fatty acids, which have a greater potential for peroxidation. The objective of this experiment was to determine the effects of peroxidized corn oil on broiler performance, dietary AMEn, and abdominal fat pad weight. The same refined corn oil sample was divided into 3 subsamples, 2 of which were exposed to different peroxidative processes. The 3 diets contained the unperoxidized corn oil (UO), a slowly peroxidized corn oil (SO; heated for 72 h at 95 degrees C with compressed air flow rate of 12 L/min), or a rapidly peroxidized corn oil (RO; heated for 12 h at 185 degrees C with compressed air flow rate of 12 L/min). Diets were fed from 0 to 14 d of age with each lipid fed at a 5% inclusion rate, continuing on from 15 to 27 d of age with each lipid fed at a 10% inclusion rate. There were 6 Ross 708 broiler chicks per cage with 10 replicates for each of the 3 dietary treatments. Abdominal fat pad and excreta collection was performed on d 27. Body weight gain, feed intake and feed efficiency were measured for the 0 to 14 and 0 to 27 d periods. The increased level of peroxidation reduced AMEn in broiler diets (UO = 3,490 kcal/kg; SO = 3,402 kcal/kg; RO = 3,344 kcal/kg on an as-is basis; SEM = 12.9, P <= 0.01). No significant treatment differences were observed among oil supplemented birds for BW gain, feed intake, feed efficiency, or abdominal fat pad weight. In conclusion, corn oil peroxidation status resulted in a decrease in dietary AMEn, but had minimal effects on broiler performance or fat pad weights. C1 [Ehr, I. J.; Persia, M. E.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Kerr, B. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Persia, ME (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. EM brian.kerr@ars.usda.gov FU Iowa State University Ag Experiment Station FX This research was financially supported in part by the Iowa State University Ag Experiment Station. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by Iowa State University or the USDA, and does not imply approval to the exclusion of other products that may be suitable. The authors gratefully acknowledge the assistance of J. Cook (USDA-ARS, Ames) with laboratory analysis, and M. Hansen, A. Gareis, and N. Nachtrieb (ISU, Ames) for animal care assistance. The USDA is an equal opportunity provider and employer. NR 29 TC 2 Z9 3 U1 0 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUL PY 2015 VL 94 IS 7 BP 1629 EP 1634 DI 10.3382/ps/pev131 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CK9BX UT WOS:000356536500024 PM 26015587 ER PT J AU Gast, RK Guraya, R Jones, DR Anderson, KE AF Gast, Richard K. Guraya, Rupa Jones, Deana R. Anderson, Kenneth E. TI Persistence of fecal shedding of Salmonella Enteritidis by experimentally infected laying hens housed in conventional or enriched cages SO POULTRY SCIENCE LA English DT Article DE Salmonella Enteritidis; chickens; fecal shedding; conventional cages; enriched colony cages ID ENTERICA SEROTYPE ENTERITIDIS; DIFFERENT HOUSING SYSTEMS; NATIONAL CONTROL PROGRAM; COMMERCIAL LAYER FLOCKS; HORIZONTAL TRANSMISSION; RISK-FACTORS; EGG CONTAMINATION; OBLIGATORY VACCINATION; SEROVAR-ENTERITIDIS; INTERNAL ORGANS AB Salmonella Enteritidis can be deposited inside eggs laid by infected hens, so the prevalence of this pathogen in commercial egg-producing flocks is an important risk factor for human illness. Opportunities for the introduction, transmission, and persistence of salmonellae in poultry are potentially influenced by flock housing and management systems. Animal welfare concerns have spurred the development of alternatives to traditional cage-based housing. However, the consequences of poultry housing systems for food safety have not been fully resolved by prior research. The present study assessed the effects of two different housing systems (conventional cages and colony cages enriched with perching and nesting areas) on the persistence of fecal shedding of Salmonella Enteritidis by groups of experimentally infected laying hens. In each of two trials, 136 hens were distributed among cages of both housing systems and orally inoculated with doses of 108 cfu of Salmonella Enteritidis (phage type 13a in one trial and phage type 4 in the other). At weekly intervals, samples of voided feces were collected from beneath each cage and cultured to detect Salmonella Enteritidis. Fecal shedding of Salmonella Enteritidis was detected for up to 8wk post-inoculation by hens housed in enriched colony cages and 10 wk by hens housed in conventional cages. For both trials combined, the frequency of positive fecal cultures was significantly (P < 0.05) greater for conventional cages than for enriched colony cages at 1wk (84.7 vs. 71.5%), 2 wk (54.2 vs. 31.3%), 3 wk (21.5 vs. 7.6%), and 4wk (9.7 vs. 2.8%) post-inoculation. These results demonstrate that the susceptibility of hens to intestinal colonization by Salmonella Enteritidis can differ between conventional and enriched cagebased production systems, although this effect does not necessarily translate into a corresponding difference in the longer-term persistence of fecal shedding. C1 [Gast, Richard K.; Guraya, Rupa; Jones, Deana R.] USDA ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. [Anderson, Kenneth E.] N Carolina State Univ, Dept Poultry Sci, Raleigh, NC 27695 USA. RP Gast, RK (reprint author), USDA ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. EM Richard.Gast@ars.usda.gov NR 64 TC 1 Z9 1 U1 2 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUL PY 2015 VL 94 IS 7 BP 1650 EP 1656 DI 10.3382/ps/pev113 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CK9BX UT WOS:000356536500027 PM 25910903 ER PT J AU Bowker, B Zhuang, H AF Bowker, B. Zhuang, H. TI Relationship between water-holding capacity and protein denaturation in broiler breast meat SO POULTRY SCIENCE LA English DT Article DE breast meat; myofibrillar; protein solubility; sarcoplasmic; water-holding capacity ID PECTORALIS MAJOR MUSCLE; MYOFIBRILLAR PROTEINS; CHICKEN MUSCLE; QUALITY TRAITS; EXUDATIVE MEAT; PORK QUALITY; FIBER TYPES; PALE; SOFT; GLYCOLYSIS AB The objective of this study was to determine the relationship between water-holding capacity (WHC) attributes and protein denaturation in broiler breast meat. Boneless skinless breast fillets (n = 72) were collected from a commercial processing plant at 2 h postmortem and segregated into low-WHC and high-WHC groups based on muscle pH and color (L*a*b*). At 6 and 24 h postmortem, brine uptake (%), cooking loss (%), and protein solubility (sarcoplasmic and myofibrillar) were measured and protein fractions were analyzed using SDS-PAGE. Drip loss accumulation (%) was measured after storage for 2 and 7 days postmortem. High-WHC fillets exhibited lower L*-lightness values and greater pH values at 2 and 24 h postmortem than low-WHC fillets. High-WHC fillets had greater brine uptake and less cooking loss at both 6 and 24 h postmortem compared to low-WHC fillets. Aging from 6 to 24 h postmortem increased brine uptake in highWHC fillets, but did not affect cooking loss in either low-WHC or high-WHC fillets. Drip loss accu-mulation was greater in low-WHC fillets at both 2 and 7 days postmortem. Myofibrillar protein solubility decreased with postmortem time but was not different between low-WHC and high-WHC fillets. Sarcoplasmic protein solubility increased with postmortem time and was greater in high-WHC fillets. SDS-PAGE analysis indicated that low-WHC fillets exhibited more glycogen phosphorylase denaturation than high-WHC fillets as evidenced by a more extensive shift of the protein from the sarcoplasmic to the myofibrillar protein fraction. Correlation analysis revealed that overall protein solubility measurements were not related to WHC attributes but that the degree of glycogen phosphorylase denaturation was significantly correlated (vertical bar r vertical bar = 0.52 to 0.80) to measures of WHC. Data indicated that WHC differences in broiler breast fillets were not due to differences in myofibrillar protein denaturation and suggested that the denaturation of sarcoplasmic proteins onto myofibrils may influence WHC in breast meat. C1 [Bowker, B.; Zhuang, H.] USDA ARS, US Natl Poultry Res Ctr, Athens, GA 30605 USA. RP Bowker, B (reprint author), USDA ARS, US Natl Poultry Res Ctr, Athens, GA 30605 USA. EM brian.bowker@ars.usda.gov NR 32 TC 1 Z9 2 U1 9 U2 37 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUL PY 2015 VL 94 IS 7 BP 1657 EP 1664 DI 10.3382/ps/pev120 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CK9BX UT WOS:000356536500028 PM 26009757 ER PT J AU McRoberts, RE Naesset, E Gobakken, T Bollandsas, OM AF McRoberts, Ronald E. Naesset, Erik Gobakken, Terje Bollandsas, Ole Martin TI Indirect and direct estimation of forest biomass change using forest inventory and airborne laser scanning data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Simple random sampling estimator; Stratified estimator; Model-assisted regression estimator ID ASSISTED ESTIMATION; STEM VOLUME; HEIGHT; LIDAR; AREA AB Remote sensing-based change estimation typically takes two forms. Indirect estimation entails constructing models of the relationship between the response variable of interest and remotely sensed auxiliary variables at two times and then estimating change as the differences in the model predictions for the two times. Direct estimation entails constructing models of change directly using observations of change in the response and the remotely sensed auxiliary variables for two dates. The direct method is generally preferred, although few statistically rigorous comparisons have been reported. This study focused on statistically rigorous, indirect and direct estimation of biomass change using forest inventory and airborne laser scanning (ALS) data for a Norwegian study area. Three sets of statistical estimators were used: simple random sampling estimators, indirect model-assisted regression estimators, and direct model-assisted regression estimators. In addition, three modeling approaches were used to support the direct model-assisted estimators. The study produced four relevant findings. First, use of the ALS auxiliary information greatly increased the precision of change estimates, regardless of whether indirect or direct methods were used. Second, contrary to previously reported results, the indirect method produced greater precision for the study area mean than the traditional direct method. Third, the direct method that used models whose predictor variables were selected in pairs but with separate coefficient estimates and models whose predictor variables were selected without regard to pairing produced the greatest precision. Finally, greater emphasis should be placed on the effects of model extrapolations for values of independent variables in the population that are beyond the range of the variables in the sample. Published by Elsevier Inc. C1 [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Naesset, Erik; Gobakken, Terje; Bollandsas, Ole Martin] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, As, Norway. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us NR 17 TC 11 Z9 13 U1 6 U2 20 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUL PY 2015 VL 164 BP 36 EP 42 DI 10.1016/j.rse.2015.02.018 PG 7 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CK9IU UT WOS:000356554600004 ER PT J AU Jansen, C Zhang, YZ Liu, HJ Gonzalez-Portilla, PJ Lauter, N Kumar, B Trucillo-Silva, I Martin, JPS Lee, M Simcox, K Schussler, J Dhugga, K Lubberstedt, T AF Jansen, Constantin Zhang, Yongzhong Liu, Hongjun Gonzalez-Portilla, Pedro J. Lauter, Nick Kumar, Bharath Trucillo-Silva, Ignacio Martin, Juan Pablo San Lee, Michael Simcox, Kevin Schussler, Jeff Dhugga, Kanwarpal Luebberstedt, Thomas TI Genetic and agronomic assessment of cob traits in corn under low and normal nitrogen management conditions SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID CONFIDENCE-INTERVALS; QTL ANALYSIS; MAIZE; ARCHITECTURE; POPULATION; LOCI; PREDICTION; MOISTURE; PROGENY; TESTERS AB Exploring and understanding the genetic basis of cob biomass in relation to grain yield under varying nitrogen management regimes will help breeders to develop dual-purpose maize. With rising energy demands and costs for fossil fuels, alternative energy from renewable sources such as maize cobs will become competitive. Maize cobs have beneficial characteristics for utilization as feedstock including compact tissue, high cellulose content, and low ash and nitrogen content. Nitrogen is quantitatively the most important nutrient for plant growth. However, the influence of nitrogen fertilization on maize cob production is unclear. In this study, quantitative trait loci (QTL) have been analyzed for cob morphological traits such as cob weight, volume, length, diameter and cob tissue density, and grain yield under normal and low nitrogen regimes. 213 doubled-haploid lines of the intermated B73 x Mo17 (IBM) Syn10 population have been resequenced for 8575 bins, based on SNP markers. A total of 138 QTL were found for six traits across six trials using composite interval mapping with ten cofactors and empirical comparison-wise thresholds (P = 0.001). Despite moderate to high repeatabilities across trials, few QTL were consistent across trials and overall levels of explained phenotypic variance were lower than expected some of the cob trait x trial combinations (R (2) = 7.3-43.1 %). Variation for cob traits was less affected by nitrogen conditions than by grain yield. Thus, the economics of cob usage under low nitrogen regimes is promising. C1 [Jansen, Constantin; Zhang, Yongzhong; Gonzalez-Portilla, Pedro J.; Kumar, Bharath; Trucillo-Silva, Ignacio; Lee, Michael; Luebberstedt, Thomas] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Zhang, Yongzhong; Liu, Hongjun] Sichuan Agr Univ, Maize Res Inst, Wenjiang 611130, Peoples R China. [Lauter, Nick] Iowa State Univ, USDA ARS Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Martin, Juan Pablo San; Simcox, Kevin; Schussler, Jeff; Dhugga, Kanwarpal] Pioneer HiBred Int Inc, Johnston, IA USA. RP Zhang, YZ (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM zhangyz2005111@163.com; thomasl@iastate.edu FU USDA_s National Institute of Food and Agriculture [IOW05180]; Interdepartmental Genetics Graduate Program; RF Baker Center for Plant Breeding at Iowa State University; China Scholarship Council FX The authors would like to acknowledge Pioneer Hi-Bred for growing the IBMsyn10 population at Marion, IA in 2010 and for providing phenotyping capacities at that location. The Authors also would like to thank USDA_s National Institute of Food and Agriculture (project number: IOW05180) for funding this work. Constantin Jansen and Pedro J. Gonzalez-Portilla were supported by the Interdepartmental Genetics Graduate Program as well as RF Baker Center for Plant Breeding at Iowa State University. Yongzhong Zhang and Hongjun Liu were the visiting student at ISU, supported by China Scholarship Council. NR 34 TC 1 Z9 1 U1 3 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2015 VL 128 IS 7 BP 1231 EP 1242 DI 10.1007/s00122-015-2486-0 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CK3WT UT WOS:000356148000002 PM 25762132 ER PT J AU Vazquez, MD Zemetra, R Peterson, CJ Chen, XMM Heesacker, A Mundt, CC AF Vazquez, M. Dolores Zemetra, Robert Peterson, C. James Chen, Xianming M. Heesacker, Adam Mundt, Christopher C. TI Multi-location wheat stripe rust QTL analysis: genetic background and epistatic interactions SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; QUANTITATIVE TRAIT LOCI; ADULT-PLANT RESISTANCE; STEM RUST; DURABLE RESISTANCE; UNITED-STATES; SPRING WHEAT; DURUM-WHEAT; LEAF RUST; MAIN AB Epistasis and genetic background were important influences on expression of stripe rust resistance in two wheat RIL populations, one with resistance conditioned by two major genes and the other conditioned by several minor QTL. Stripe rust is a foliar disease of wheat (Triticum aestivum L.) caused by the air-borne fungus Puccinia striiformis f. sp. tritici and is present in most regions around the world where commercial wheat is grown. Breeding for durable resistance to stripe rust continues to be a priority, but also is a challenge due to the complexity of interactions among resistance genes and to the wide diversity and continuous evolution of the pathogen races. The goal of this study was to detect chromosomal regions for resistance to stripe rust in two winter wheat populations, 'Tubbs'/'NSA-98-0995' (T/N) and 'Einstein'/'Tubbs' (E/T), evaluated across seven environments and mapped with diversity array technology and simple sequence repeat markers covering polymorphic regions of a parts per thousand 1480 and 1117 cM, respectively. Analysis of variance for phenotypic data revealed significant (P < 0.01) genotypic differentiation for stripe rust among the recombinant inbred lines. Results for quantitative trait loci/locus (QTL) analysis in the E/T population indicated that two major QTL located in chromosomes 2AS and 6AL, with epistatic interaction between them, were responsible for the main phenotypic response. For the T/N population, eight QTL were identified, with those in chromosomes 2AL and 2BL accounting for the largest percentage of the phenotypic variance. C1 [Vazquez, M. Dolores; Mundt, Christopher C.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Zemetra, Robert; Heesacker, Adam] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Peterson, C. James] Limagrain Cereals Seeds, Ft Collins, CO 80524 USA. [Chen, Xianming M.] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Dept Plant Pathol, Pullman, WA 99164 USA. RP Vazquez, MD (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM m.dolores.vazquez@oregonstate.edu FU Oregon Wheat Commission; Warren Kronstad Endowment FX We thank the Oregon Wheat Commission and Warren Kronstad Endowment for funding the project. We thank the Columbia Basin Agricultural Research Center staff, Kathryn Sackett, Larae Wallace, Mark Larson, and Paul Severns for their excellent technical assistance. NR 56 TC 3 Z9 3 U1 2 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2015 VL 128 IS 7 BP 1307 EP 1318 DI 10.1007/s00122-015-2507-z PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CK3WT UT WOS:000356148000007 PM 25847212 ER PT J AU Lin, M Cai, SB Wang, S Liu, SB Zhang, GR Bai, GH AF Lin, Meng Cai, Shibin Wang, Shan Liu, Shubing Zhang, Guorong Bai, Guihua TI Genotyping-by-sequencing (GBS) identified SNP tightly linked to QTL for pre-harvest sprouting resistance SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; CONTROLLING SEED DORMANCY; GRAIN DORMANCY; CHROMOSOME 4A; MAPPING QTLS; WHITE WHEAT; MAJOR QTL; BARLEY; MARKERS; MICROSATELLITE AB Using a GBS-SNP map, a QTL for pre-harvest sprouting resistance on 4AL of Totoumai A was delimited to 2.9-cM interval, and SNP closely linked to several other QTL were identified. Pre-harvest sprouting (PHS) of wheat is a major constraint to wheat production in many wheat-growing areas worldwide, because it reduces both wheat grain yield and the end-use quality. To identify markers tightly linked to the quantitative trait loci (QTL) for PHS resistance and seed dormancy (SD), we evaluated 155 recombinant inbred lines (RIL) derived from a cross between a PHS-resistant parent 'Tutoumai A' and a PHS-susceptible parent 'Siyang 936' for single-nucleotide polymorphisms (SNP) using genotyping-by-sequencing (GBS), and for PHS resistance and SD using both field and greenhouse grown plants. Two SNP, GBS109947 and GBS212432, were mapped to a major QTL region for PHS resistance and SD on chromosome 4AL, and delimited the QTL to a 2.9-cM interval. Two and nine additional SNP were mapped to minor QTL regions for SD on chromosome 5B and 5A, respectively. Critical SNP in these QTL regions were converted into KBioscience Competitive Allele-Specific PCR (KASP) assays that can be easily used for marker-assisted selection to improve PHS resistance. C1 [Lin, Meng; Cai, Shibin; Wang, Shan; Liu, Shubing; Zhang, Guorong; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Cai, Shibin] Jiangsu Acad Agr Sci, Food Crop Inst, Nanjing, Peoples R China. RP Bai, GH (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. EM gbai@ksu.edu FU National Research Initiative Competitive Grants CAP from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This is contribution number 15-199-J from the Kansas Agricultural Experiment Station. This project is partly funded by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 46 TC 8 Z9 9 U1 8 U2 67 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2015 VL 128 IS 7 BP 1385 EP 1395 DI 10.1007/s00122-015-2513-1 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CK3WT UT WOS:000356148000013 PM 25851002 ER PT J AU Pujol, V Forrest, KL Zhang, P Rouse, MN Hayden, MJ Huang, L Tabe, L Lagudah, E AF Pujol, Vincent Forrest, Kerrie L. Zhang, Peng Rouse, Matthew N. Hayden, Matthew J. Huang, Li Tabe, Linda Lagudah, Evans TI Identification of a stem rust resistance locus effective against Ug99 on wheat chromosome 7AL using a RAD-Seq approach SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; HEXAPLOID WHEAT; COMMON WHEAT; NONSUPPRESSOR MUTATION; POWDERY MILDEW; RACE UG99; GENE; CANTHATCH; MARKERS; SUPPRESSION AB A locus of major effect for stem rust resistance, effective against Ug99 and possibly a target of a suppressor on chromosome arm 7DL in wheat cultivar Canthatch, was mapped to 7AL. Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is responsible for major production losses around the world. The development of resistant cultivars is an effective and environmentally friendly way to manage the disease, but outbreaks can occur when new pathogen races overcome the existing host resistance genes. Ug99 (race TTKSK) and related Pgt races are virulent to the majority of existing cultivars, which presents a potential threat to global wheat production. The hexaploid wheat cultivar Canthatch has long been known to carry a suppressor of stem rust resistance on chromosome arm 7DL. Multiple "non-suppressor" mutants of Canthatch are reported to have gained resistance to Pgt races, including Ug99 (TTKSK) and related races TTKST and TTTSK. To genetically map the suppressor locus, a mapping population was developed from a cross between the susceptible cultivar Columbus, thought to possess the suppressor, and Columbus-NS766, a resistant, near-isogenic line believed to contain a mutant non-suppressor allele introgressed from Canthatch. Genetic mapping using a 9K SNP genotyping assay and restriction site-associated DNA sequencing (RAD-Seq) on bulked segregants led to the identification of markers linked to a locus of stem rust resistance. Surprisingly, genomic sequence information revealed the markers to be located on 7AL instead of 7DL, indicating that the resistance phenotype was due to a new resistance locus, rather than the inactivated suppressor. We suggest that the 7AL locus of resistance is most likely suppressed by the 7DL suppressor. C1 [Pujol, Vincent; Tabe, Linda; Lagudah, Evans] CSIRO Plant Ind, Canberra, ACT 2601, Australia. [Forrest, Kerrie L.; Hayden, Matthew J.] Ctr AgriBiosci, Dept Environm & Primary Ind Agribio, Bundoora, Vic 3083, Australia. [Zhang, Peng] Univ Sydney, Plant Breeding Inst, Cobbitty, NSW 2570, Australia. [Rouse, Matthew N.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Huang, Li] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. RP Pujol, V (reprint author), CSIRO Plant Ind, GPO Box 1600, Canberra, ACT 2601, Australia. EM pujol@affrc.go.jp RI Rouse, Matthew/G-5474-2011 FU BREAD program of the National Science Foundation [4W3101] FX We thank Prof. Robert McIntosh for his advice on rust phenotyping, Dr. Sami Hoxha for coordinating phenotyping experiments in Australia, Dr. Stephan Kong and Debbie Wong for their assistance in the construction of RAD-tag libraries and in the 9K SNP genotyping assay, and Dr. Kerber for providing the Col and CTH lines. This study was funded by the BREAD program of the National Science Foundation (4W3101). NR 35 TC 1 Z9 1 U1 3 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2015 VL 128 IS 7 BP 1397 EP 1405 DI 10.1007/s00122-015-2514-0 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CK3WT UT WOS:000356148000014 PM 25877521 ER PT J AU Eberhardt, TL Samuelson, LJ AF Eberhardt, Thomas L. Samuelson, Lisa J. TI Collection of wood quality data by X-ray densitometry: a case study with three southern pines SO WOOD SCIENCE AND TECHNOLOGY LA English DT Article ID RING SPECIFIC-GRAVITY; LOBLOLLY-PINE; RADIAL GROWTH; LONGLEAF PINE; NORWAY SPRUCE; UNITED-STATES; DOUGLAS-FIR; MATURE WOOD; CAMBIAL AGE; SLASH PINE AB X-ray densitometry is a technique often used in tree growth and wood quality studies to incrementally measure density (specific gravity) along a radial strip of wood. Protocols for this technique vary between laboratories because of differences in species, equipment, tree age, and other factors. Here, the application of X-ray densitometry is discussed in terms of a case study specific to the southern pines, whereby loblolly (Pinus taeda L), longleaf (Pinus palustris Mill.), and slash (Pinus elliottii Engelm.) pine wood cores were analyzed using an automated system. Objectives of this study included an assessment of the potential impacts on whole-core wood quality data from wood core extraction and use of two different demarcation methods (threshold and inflection point) for the earlywood-latewood transition. Wood core extraction before X-ray densitometry showed minimal impact on the shapes of individual ring profiles, and whole-core wood quality data were essentially unchanged. An assessment of the inflection point method employed for determining the earlywood-latewood transition point in the X-ray densitometry data demonstrated that the growth ring profiles for the southern pines are not amenable to polynomial fitting. Indeed, in the southern pines, the earlywood-latewood transitions are as equally abrupt as the latewood-earlywood transitions. Accordingly, a threshold density deemed appropriate to define the onset of a growth ring would be equally appropriate to define the onset of latewood formation. C1 [Eberhardt, Thomas L.] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Samuelson, Lisa J.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. RP Eberhardt, TL (reprint author), US Forest Serv, Forest Prod Lab, USDA, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM teberhardt@fs.fed.us NR 49 TC 0 Z9 0 U1 9 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0043-7719 EI 1432-5225 J9 WOOD SCI TECHNOL JI Wood Sci. Technol. PD JUL PY 2015 VL 49 IS 4 BP 739 EP 753 DI 10.1007/s00226-015-0732-x PG 15 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA CK3EE UT WOS:000356098600008 ER PT J AU Fitzpatrick, AL Rapp, SR Luchsinger, J Hill-Briggs, F Alonso, A Gottesman, R Lee, H Carnethon, M Liu, K Williams, K Sharrett, AR Frazier-Wood, A Lyketsos, C Seeman, T AF Fitzpatrick, Annette L. Rapp, Stephen R. Luchsinger, Jose Hill-Briggs, Felicia Alonso, Alvaro Gottesman, Rebecca Lee, Hochang Carnethon, Mercedes Liu, Kiang Williams, Kayleen Sharrett, A. Richey Frazier-Wood, Alexis Lyketsos, Constantine Seeman, Teresa TI Sociodemographic Correlates of Cognition in the Multi-Ethnic Study of Atherosclerosis (MESA) SO AMERICAN JOURNAL OF GERIATRIC PSYCHIATRY LA English DT Article DE MESA; cognition; methods; multi-ethnic; race; socioeconomic ID AFRICAN-AMERICANS; KAME PROJECT; KING COUNTY; DEMENTIA; EDUCATION; PERFORMANCE; PREVALENCE; NEUROPSYCHOLOGY; LITERACY; DECLINE AB Objective: To describe the methodology utilized to evaluate cognitive function in the Multi-Ethnic Study of Atherosclerosis (MESA) and to present preliminary results by age, sex, and race/ethnicity. Design: Cross-sectional measurements of a prospective observational cohort. Setting: Residents of 6 U.S. communities free of cardiovascular disease at baseline (2000-02). Participants: 4,591 adults who completed the fifth MESA clinical examination in 2011-12; mean age 70.3 (SD: 9.5) years, 53.1% women, 40.7% non-Hispanic white, 26.4% non-Hispanic black, 21.4% Hispanic, and 11.5% Chinese. Measurements: The cognitive battery consisted of the Cognitive Abilities Screening Instrument (version 2) to evaluate global cognition, the Digit Symbol Code for processing speed and Digit Spans Forward and Backward to assess memory. Demographic, socioeconomic, and cultural covariates were also collected for descriptive statistics and multivariate modeling. Results: Associations between socioeconomic factors and cognition revealed that age, race/ethnicity, education, occupational status, household income, health insurance type, household size, place of birth, years and generation in U.S., and the presence of the ApoE4 allele were significantly associated with performance on the cognitive tests, although patterns varied by specific test, racial/ethnicity, and sociocultural factors. Conclusion: As many of the influencing cultural and socioeconomic factors measured here are complex, multifactorial, and may not be adequately quantified, caution has been C1 [Fitzpatrick, Annette L.] Univ Washington, Dept Family Med, Seattle, WA 98195 USA. [Fitzpatrick, Annette L.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Fitzpatrick, Annette L.] Univ Washington, Dept Global Hlth, Seattle, WA 98195 USA. [Rapp, Stephen R.] Wake Forest Univ, Bowman Gray Sch Med, Dept Psychiat & Behav Med, Winston Salem, NC USA. [Luchsinger, Jose] Columbia Univ, Dept Med, Med Ctr, New York, NY USA. [Luchsinger, Jose] Columbia Univ, Med Ctr, Dept Epidemiol, New York, NY USA. [Hill-Briggs, Felicia] Johns Hopkins Med Inst, Dept Med, Baltimore, MD 21205 USA. [Hill-Briggs, Felicia] Johns Hopkins Med Inst, Dept Hlth Behav & Soc, Baltimore, MD 21205 USA. [Alonso, Alvaro] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Gottesman, Rebecca] Johns Hopkins Univ Hosp, Dept Cerebrovasc Neurol, Baltimore, MD 21287 USA. [Lee, Hochang] Yale Univ, Sch Med, Psychol Med Serv, New Haven, CT USA. [Carnethon, Mercedes; Liu, Kiang] Northwestern Univ, Feinberg Sch Med, Dept Prevent Med, Chicago, IL 60611 USA. [Williams, Kayleen] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Sharrett, A. Richey] Johns Hopkins Univ, Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Frazier-Wood, Alexis] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lyketsos, Constantine] Johns Hopkins Univ, Dept Psychiat & Behav Sci, Baltimore, MD USA. [Seeman, Teresa] Univ Calif Los Angeles, Dept Med, Los Angeles, CA 90024 USA. [Seeman, Teresa] Univ Calif Los Angeles, Dept Epidemiol, Los Angeles, CA 90024 USA. RP Fitzpatrick, AL (reprint author), Univ Washington, Dept Family Med, Box 357236, Seattle, WA 98195 USA. EM fitzpal@u.washington.edu RI Alonso, Alvaro/A-4917-2010 OI Alonso, Alvaro/0000-0002-2225-8323 FU National Heart, Lung, and Blood Institute [N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, N01-HC-95169]; National Center for Research Resources [UL1-TR-000040, UL1-RR-025005]; National Institute on Minority Health and Health Disparities [P60 MD 00206]; MESA FX This research was supported by contracts N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, and N01-HC-95169 from the National Heart, Lung, and Blood Institute; by grants UL1-TR-000040 and UL1-RR-025005 from the National Center for Research Resources; and by P60 MD 00206 from the National Institute on Minority Health and Health Disparities. The authors thank the other investigators, the staff, and the participants of the MESA study for their valuable contributions. A full list of participating MESA investigators and institutions can be found at http://www.mesa-nhlbi.org. MESA provides funding through one or more of these sources to Drs. Fitzpatrick, Alonso, Carnethon, Gottesman, Rapp, Seeman, Sharrett, and Ms. Williams. No authors have conflicts of interest to report. NR 28 TC 3 Z9 4 U1 1 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1064-7481 EI 1545-7214 J9 AM J GERIAT PSYCHIAT JI Am. J. Geriatr. Psychiatr. PD JUL PY 2015 VL 23 IS 7 BP 684 EP 697 DI 10.1016/j.jagp.2015.01.003 PG 14 WC Geriatrics & Gerontology; Gerontology; Psychiatry SC Geriatrics & Gerontology; Psychiatry GA CK0WN UT WOS:000355926200005 PM 25704999 ER PT J AU Lambertini, E Karns, JS Van Kessel, JAS Cao, HL Schukken, YH Wolfgang, DR Smith, JM Pradhan, AK AF Lambertini, Elisabetta Karns, Jeffrey S. Van Kessel, Jo Ann S. Cao, Huilin Schukken, Ynte H. Wolfgang, David R. Smith, Julia M. Pradhan, Abani K. TI Dynamics of Escherichia coli Virulence Factors in Dairy Herds and Farm Environments in a Longitudinal Study in the United States SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BULK TANK MILK; HEMOLYTIC-UREMIC SYNDROME; HORIZONTAL GENE-TRANSFER; RAW COWS MILK; SHIGA-TOXIN; FOODBORNE PATHOGENS; FEEDLOT CATTLE; LISTERIA-MONOCYTOGENES; SALMONELLA-ENTERICA; PASTEURIZED MILK AB Pathogenic Escherichia coli or its associated virulence factors have been frequently detected in dairy cow manure, milk, and dairy farm environments. However, it is unclear what the long-term dynamics of E. coli virulence factors are and which farm compartments act as reservoirs. This study assessed the occurrence and dynamics of four E. coli virulence factors (eae, stx(1), stx(2), and the gamma allele of the tir gene [gamma-tir]) on three U.S. dairy farms. Fecal, manure, water, feed, milk, and milk filter samples were collected from 2004 to 2012. Virulence factors were measured by postenrichment quantitative PCR (qPCR). All factors were detected in most compartments on all farms. Fecal and manure samples showed the highest prevalence, up to 53% for stx and 21% for gamma-tir in fecal samples and up to 84% for stx and 44% for gamma-tir in manure. Prevalence was low in milk (up to 1.9% for stx and 0.7% for gamma-tir). However, 35% of milk filters were positive for stx and 20% were positive for gamma-tir. All factors were detected in feed and water. Factor prevalence and levels, expressed as qPCR cycle threshold categories, fluctuated significantly over time, with no clear seasonal signal independent from year-to-year variability. Levels were correlated between fecal and manure samples, and in some cases autocorrelated, but not between manure and milk filters. Shiga toxins were nearly ubiquitous, and 10 to 18% of the lactating cows were potential shedders of E. coli O157 at least once during their time in the herds. E. coli virulence factors appear to persist in many areas of the farms and therefore contribute to transmission dynamics. C1 [Lambertini, Elisabetta; Cao, Huilin; Pradhan, Abani K.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Lambertini, Elisabetta; Pradhan, Abani K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Karns, Jeffrey S.; Van Kessel, Jo Ann S.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD USA. [Schukken, Ynte H.] Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY USA. [Schukken, Ynte H.] GD Anim Hlth, Deventer, Netherlands. [Wolfgang, David R.] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Smith, Julia M.] Univ Vermont, Dept Anim Sci, Burlington, VT 05405 USA. RP Pradhan, AK (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM akp@umd.edu OI Schukken, Ynte/0000-0002-8250-4194 NR 101 TC 1 Z9 1 U1 3 U2 22 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2015 VL 81 IS 13 BP 4477 EP 4488 DI 10.1128/AEM.00465-15 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CJ9TC UT WOS:000355844800025 PM 25911478 ER PT J AU Noar, J Loveless, T Navarro-Herrero, JL Olson, JW Bruno-Barcena, JM AF Noar, Jesse Loveless, Telisa Luis Navarro-Herrero, Jose Olson, Jonathan W. Bruno-Barcena, Jose M. TI Aerobic Hydrogen Production via Nitrogenase in Azotobacter vinelandii CA6 SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID AMMONIUM-ASSIMILATING CULTURES; 2ND ALTERNATIVE NITROGENASE; RHODOBACTER-CAPSULATUS; THERMOTOGA-NEAPOLITANA; STRUCTURAL GENES; TRANSCRIPTIONAL REGULATION; ENTEROBACTER-AEROGENES; KLEBSIELLA-PNEUMONIAE; MOLYBDENUM TRANSPORT; MUTATIONAL ANALYSIS AB The diazotroph Azotobacter vinelandii possesses three distinct nitrogenase isoenzymes, all of which produce molecular hydrogen as a by-product. In batch cultures, A. vinelandii strain CA6, a mutant of strain CA, displays multiple phenotypes distinct from its parent: tolerance to tungstate, impaired growth and molybdate transport, and increased hydrogen evolution. Determining and comparing the genomic sequences of strains CA and CA6 revealed a large deletion in CA6's genome, encompassing genes related to molybdate and iron transport and hydrogen reoxidation. A series of iron uptake analyses and chemostat culture experiments confirmed iron transport impairment and showed that the addition of fixed nitrogen (ammonia) resulted in cessation of hydrogen production. Additional chemostat experiments compared the hydrogen-producing parameters of different strains: in iron-sufficient, tungstate-free conditions, strain CA6's yields were identical to those of a strain lacking only a single hydrogenase gene. However, in the presence of tungstate, CA6 produced several times more hydrogen. A. vinelandii may hold promise for developing a novel strategy for production of hydrogen as an energy compound. C1 [Noar, Jesse; Bruno-Barcena, Jose M.] N Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA. [Olson, Jonathan W.] N Carolina State Univ, Dept Biol Sci, Raleigh, NC 27695 USA. [Loveless, Telisa] USDA ARS, NCSU, Raleigh, NC 27695 USA. [Luis Navarro-Herrero, Jose] Univ Politecn Valencia, Dept Automat & Syst Engn, Valencia, Spain. RP Bruno-Barcena, JM (reprint author), N Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA. EM jbbarcen@ncsu.edu OI Noar, Jesse/0000-0001-5879-6468 FU 3-year National Science Foundation Graduate Research Fellowship; Plant and Microbial Biology; [NIH P30 DK34987] FX J.N. was the recipient of a 3-year National Science Foundation Graduate Research Fellowship. This project was supported by the North Carolina State University Department of Microbiology (now Plant and Microbial Biology). The University of North Carolina-Chapel Hill Core facility is supported by grant number NIH P30 DK34987. NR 54 TC 1 Z9 1 U1 3 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2015 VL 81 IS 13 BP 4507 EP 4516 DI 10.1128/AEM.00679-15 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CJ9TC UT WOS:000355844800028 PM 25911479 ER PT J AU Lee, KJ Rahimnejad, S Powell, MS Barrows, FT Smiley, S Bechtel, PJ Hardy, RW AF Lee, Kyeong-Jun Rahimnejad, Samad Powell, Madison S. Barrows, Frederick T. Smiley, Scott Bechtel, Peter J. Hardy, Ronald W. TI Salmon testes meal as a functional feed additive in fish meal and plant protein-based diets for rainbow trout (Oncorhynchus mykiss Walbaum) and Nile tilapia (Oreochromis niloticus L.) fry SO AQUACULTURE RESEARCH LA English DT Article DE fish meal; plant protein; testes meal; rainbow trout; Nile tilapia ID FISHERIES BY-CATCH; NUTRITIONAL-VALUE; BODY-COMPOSITION; PRODUCT MEALS; GROWTH; HORMONES; 17-ALPHA-METHYLTESTOSTERONE; NUCLEOTIDES; SUPPLEMENTATION; JUVENILES AB Fishery processing by-products are a large resource from which to produce fishmeal and other products for a variety of uses. In this study, testes meal (TM) produced from pink salmon processing by-product was evaluated as a functional ingredient in aquafeeds. Nile tilapia and rainbow trout fry were fed five isonitrogenous and isoenergetic experimental diets for 4 and 9weeks respectively. Two diets were fishmeal-based (FM) and three were plant protein-based (PP). Salmon TM was added to the FM and PP diets at 7% to replace 20% of fishmeal protein (FMTM and PPTM respectively). An additional control diet was prepared in which fishmeal was added to the PP diet to supply an equivalent amount of protein as supplied by TM (PPFM). Inclusion of TM in both the FM- and PP-based diets resulted in higher final body weights, although differences were only significant between rainbow trout fed FM or FMTM diets. Similar differences were calculated for other indices of fish performance, e.g. specific growth rate, feed conversion ratio, protein efficiency ratio and protein retention efficiency. Feed intake was significantly higher for fish fed FMTM compared with FM in rainbow trout. For tilapia, final weights were numerically higher, but not significantly different for fish fed diets containing TM compared with non-TM diets (FM vs. FMTM; PP vs. PPTM). Performance of trout or tilapia fed the PPFM diet did not increase compared with the PP diet. The results indicate that TM addition to both FM and PP diets increased feed intake and also increased metabolic efficiency, demonstrating that TM can be a functional ingredient in aquafeeds. C1 [Lee, Kyeong-Jun; Powell, Madison S.; Hardy, Ronald W.] Univ Idaho, Inst Aquaculture Res, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Lee, Kyeong-Jun; Rahimnejad, Samad] Jeju Natl Univ, Dept Marine Life Sci, Jeju, South Korea. [Barrows, Frederick T.] ARS, Trout Grains Project, Hagerman Fish Culture Expt Stn, USDA, Hagerman, ID USA. [Smiley, Scott] Univ Alaska Fishery Ind Technol Ctr, Kodiak, AK USA. [Bechtel, Peter J.] ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. RP Hardy, RW (reprint author), Univ Idaho, Inst Aquaculture Res, Hagerman Fish Culture Expt Stn, 3059-F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. EM rhardy@uidaho.edu FU USDA-ARS Specific Cooperative Agreement [58-5341-9-441] FX This study was funded in part by USDA-ARS Specific Cooperative Agreement # 58-5341-9-441. We thank Mike Casten and Carol Hoffman for their assistance. NR 36 TC 0 Z9 0 U1 3 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD JUL PY 2015 VL 46 IS 7 BP 1590 EP 1596 DI 10.1111/are.12313 PG 7 WC Fisheries SC Fisheries GA CK2UH UT WOS:000356068400006 ER PT J AU Renye, JA Somkuti, GA AF Renye, John A., Jr. Somkuti, George A. TI Nisin-induced expression of a recombinant antihypertensive peptide in dairy lactic acid bacteria SO BIOTECHNOLOGY LETTERS LA English DT Article DE Antihypertensive peptide; Lactic acid bacteria; Nisin-induced expression; Pediocin ID I-CONVERTING-ENZYME; STREPTOCOCCUS-THERMOPHILUS; ESCHERICHIA-COLI; MILK; INHIBITORS; PEDIOCIN; CLONING AB To improve the process for the production of milk-derived antihypertensive peptides, including a 12-residue peptide (FFVAPFPECVGK) from alpha(S1)-casein. A synthetic gene encoding this peptide was cloned within the pediocin operon, replacing the nucleic acid sequence encoding the mature pediocin peptide (papA) and resulting in a translational fusion between the pediocin leader peptide and the 12-residue hypotensive (C-12) peptide. The recombinant operon was subsequently cloned immediately downstream of the nisA promoter to allow for inducible gene expression within Streptococcus thermophilus ST128, Lactococcus lactis subsp. lactis ML3 and Lactobacillus casei C2. RT-PCR was used to confirm recombinant gene expression in complex medium; and SDS-PAGE analysis showed that the pediocin secretion machinery, encoded by papC and papD, allowed for secretion of the recombinant peptide from both L. lactis ML3 and L. casei C2 in a chemically defined medium. The use of a nisin as a "food-grade" inducer molecule, and generally-regarded-as-safe LAB species suggests that this system could be used for the production of functional food ingredients. C1 [Renye, John A., Jr.; Somkuti, George A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Renye, JA (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM john.renye@ars.usda.gov NR 20 TC 0 Z9 0 U1 1 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD JUL PY 2015 VL 37 IS 7 BP 1447 EP 1454 DI 10.1007/s10529-015-1817-1 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CJ7OL UT WOS:000355685800016 PM 25846139 ER PT J AU Laufer, AS Grass, J Holt, K Whichard, JM Griffin, PM Gould, LH AF Laufer, A. S. Grass, J. Holt, K. Whichard, J. M. Griffin, P. M. Gould, L. H. TI Outbreaks of itSalmonella infections attributed to beef-United States,1973-2011 SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Antibiotic resistance; food safety; foodborne infections; outbreaks; Salmonella enterica ID FOODBORNE DISEASE OUTBREAKS; GROUND-BEEF; SALMONELLA-TYPHIMURIUM; LYMPH-NODES; STATES; FOOD; SURVEILLANCE; PATHOGENS; ENTERICA; MEAT AB Non-typhoidal Salmonella is estimated to be the most common bacterial cause of foodborne illness in the United States, causing an estimated one million domestically acquired foodborne illnesses annually. Recent, large outbreaks have highlighted the importance of ground beef as an important source of multidrug-resistant Salmonella. We analysed the epidemiology of salmonellosis outbreaks that were attributed to beef in the United States reported to the Centers for Disease Control and Prevention (CDC) from 1973 to 2011. During 1973-2011, of the 1965 outbreaks of Salmonella where a food vehicle was implicated, 96 were attributed to beef, accounting for 3684 illnesses. We observed a shift in the type of beef implicated in salmonellosis outbreaks, from roast to ground beef. Delicatessen-style roast beef cooked in commercial processing establishments was the predominant type during the 1970s and early 1980s; regulations on cooking and processing essentially eliminated this problem by 1987. Ground beef emerged as an important vehicle in the 2000s; it was implicated in 17 (45%) of the 38 beef-attributed outbreaks reported during 2002-2011. Although this emergence was likely due in part to increased participation in CDC's PulseNet, which was established in 1996, and proactive decisions by the United States Department of Agriculture's Food Safety and Inspection Service, stronger measures are needed to decrease contamination of ground beef with Salmonella. C1 [Laufer, A. S.] CDC, Epidem Intelligence Serv, Div Sci Educ & Profess Dev, CSELS, Atlanta, GA 30333 USA. [Laufer, A. S.; Grass, J.; Griffin, P. M.; Gould, L. H.] CDC, Enter Dis Epidemiol Branch, DFWED, NCEZID, Atlanta, GA 30333 USA. [Holt, K.] USDA, Food Safety & Inspect Serv, Washington, DC 20250 USA. [Whichard, J. M.] CDC, Enter Dis Lab Branch, DFWED, NCEZID, Atlanta, GA 30333 USA. RP Laufer, AS (reprint author), Ctr Dis Control & Prevent, 1600 Clifton Rd NE,Mail Stop A-31, Atlanta, GA 30333 USA. EM ALAUFER@cdc.gov FU Intramural CDC HHS [CC999999] NR 43 TC 7 Z9 7 U1 7 U2 29 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUL PY 2015 VL 143 IS 9 BP 2003 EP 2013 DI 10.1017/S0950268814003112 PG 11 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CJ8NU UT WOS:000355760600025 PM 25427978 ER PT J AU Pedersen, K Pabilonia, KL Anderson, TD Bevins, SN Hicks, CR Kloft, JM Deliberto, TJ AF Pedersen, K. Pabilonia, K. L. Anderson, T. D. Bevins, S. N. Hicks, C. R. Kloft, J. M. Deliberto, T. J. TI Widespread detection of antibodies to Leptospira in feral swine in the United States SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Disease; feral swine; Leptospira; leptospirosis; Sus scrofa ID SUS-SCROFA; WILD BOARS; ZOONOTIC DISEASE; SEROLOGIC SURVEY; HOGS; PARTICIPANTS; CALIFORNIA; PATHOGEN; OUTBREAK; FLORIDA AB As feral swine continue to expand their geographical range and distribution across the United States, their involvement in crop damage, livestock predation, and pathogen transmission is likely to increase. Despite the relatively recent discovery of feral swine involvement in the aetiology of a variety of pathogens, their propensity to transmit and carry a wide variety of pathogens is disconcerting. We examined sera from 2055 feral swine for antibody presence to six serovars of Leptospira that can also infect humans, livestock or domestic animals. About 13% of all samples tested positive for at least one serovar, suggesting that Leptospira infection is common in feral swine. Further studies to identify the proportion of actively infected animals are needed to more fully understand the risk they pose. C1 [Pedersen, K.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Ft Collins, CO 80521 USA. [Pabilonia, K. L.; Anderson, T. D.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Bevins, S. N.; Deliberto, T. J.] USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Ft Collins, CO 80521 USA. [Kloft, J. M.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Manhattan, KS USA. RP Pedersen, K (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Kerri.Pedersen@aphis.usda.gov NR 31 TC 2 Z9 2 U1 3 U2 13 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUL PY 2015 VL 143 IS 10 SI SI BP 2131 EP 2136 DI 10.1017/S0950268814003148 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CK3ZY UT WOS:000356157400012 PM 25518910 ER PT J AU Nakao, JH Pringle, J Jones, RW Nix, BE Borders, J Heseltine, G Gomez, TM McCluskey, B Roney, CS Brinson, D Erdman, M McDaniel, A Behravesh, CB AF Nakao, J. H. Pringle, J. Jones, R. W. Nix, B. E. Borders, J. Heseltine, G. Gomez, T. M. McCluskey, B. Roney, C. S. Brinson, D. Erdman, M. McDaniel, A. Behravesh, C. Barton TI 'One Health' investigation: outbreak of human Salmonella Braenderup infections traced to a mail-order hatchery - United States, 2012-2013 SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Outbreaks; poultry; Salmonella; Salmonella enterica; zoonoses ID LIVE POULTRY; CONTACT AB Human salmonellosis linked to contact with live poultry is an increasing public health concern. In 2012, eight unrelated outbreaks of human salmonellosis linked to live poultry contact resulted in 517 illnesses. In July 2012, PulseNet, a national molecular surveillance network, reported a multistate cluster of a rare strain of Salmonella Braenderup infections which we investigated. We defined a case as infection with the outbreak strain, determined by pulsed-field gel electrophoresis, with illness onset from 25 July 2012-27 February 2013. Ill persons and mail-order hatchery (MOH) owners were interviewed using standardized questionnaires. Traceback and environmental investigations were conducted. We identified 48 cases in 24 states. Twenty-six (81%) of 32 ill persons reported live poultry contact in the week before illness; case-patients named 12 different MOHs from eight states. The investigation identified hatchery D as the ultimate poultry source. Sampling at hatchery D yielded the outbreak strain. Hatchery D improved sanitation procedures and pest control; subsequent sampling failed to yield Salmonella. This outbreak highlights the interconnectedness of humans, animals, and the environment and the importance of industry knowledge and involvement in solving complex outbreaks. Preventing these infections requires a 'One Health' approach that leverages expertise in human, animal, and environmental health. C1 [Nakao, J. H.] Ctr Dis Control & Prevent CDC, NCEZID, DFWED, EIS,EDEB, Atlanta, GA USA. [Pringle, J.; Behravesh, C. Barton] CDC, NCEZID, DFWED, ORPB, Atlanta, GA 30333 USA. [Jones, R. W.; Nix, B. E.] Texas Dept State Hlth Serv, Hlth Serv Reg 7, Temple, TX USA. [Heseltine, G.] Texas Dept State Hlth Serv, Foodborne Illness Surveillance, Emerging & Acute Infect Dis Branch, Temple, TX USA. [Gomez, T. M.; McCluskey, B.] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Riverdale, MD USA. [Roney, C. S.; Brinson, D.] USDA, APHIS, VS, NPIP, Conyers, GA USA. [Erdman, M.] USDA, Natl Vet Serv Labs, APHIS, VS,Diagnost Bacteriol Lab, Ames, IA 50010 USA. [McDaniel, A.] CDC, NCEZID, DFWED, Enter Dis Lab Branch, Atlanta, GA 30333 USA. RP Nakao, JH (reprint author), Ctr Dis Control & Prevent, Emergency Response & Recovery Branch, CGH DGHP, 4770 Buford Hwy,MSF 57, Atlanta, GA 30341 USA. EM jnakao@cdc.gov FU Epi-Aid [2013-027] FX This work was supported by Epi-Aid 2013-027. NR 20 TC 2 Z9 3 U1 1 U2 8 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUL PY 2015 VL 143 IS 10 SI SI BP 2178 EP 2186 DI 10.1017/S0950268815000151 PG 9 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CK3ZY UT WOS:000356157400016 PM 25703600 ER PT J AU Chen, C Jiang, Q Ziska, LH Zhu, JG Liu, G Zhang, JS Ni, K Seneweera, S Zhu, CW AF Chen, Chen Jiang, Qian Ziska, Lewis H. Zhu, Jianguo Liu, Gang Zhang, Jishuang Ni, Kang Seneweera, Saman Zhu, Chunwu TI Seed vigor of contrasting rice cultivars in response to elevated carbon dioxide SO FIELD CROPS RESEARCH LA English DT Article DE Cultivars; Elevated CO2; Germination rate; Germination energy; Seed vigor ID CO2 ENRICHMENT FACE; ORYZA-SATIVA L.; YIELD FORMATION; GRAIN QUALITY; GROWTH; TEMPERATURE; GERMINATION; IMPACT; ACCLIMATION; NUTRITION AB Although a number of studies have shown that rising atmospheric carbon dioxide concentration, [CO2], can differentially affect the growth and yield potential of rice (Oryza sativa L.) cultivars, there has been no attempt to determine if the response is associated with changes in seed vigor, an essential aspect of crop establishment. Because previous investigations have shown that [CO2] can change the grain structure and quality of rice seed, we hypothesized that [CO2] would decrease vigor via decreased germination rates. To test this hypothesis, we used an in situ, free-air CO2 enrichment (FACE) system to assess seed quality in six rice cultivars that differed in their growth and reproductive response to rising [CO2]. Elevated [CO2] had no effect on seed hull thickness or seed specific gravity, but did significantly reduce total nitrogen and protein concentration for all cultivars. Despite the changes in grain physical and chemical traits associated with germination, no clear indication of quantitative effects of elevated [CO2 ] on rice germination was found. (C) 2015 Elsevier B.V. All rights reserved. C1 [Chen, Chen; Jiang, Qian; Zhu, Jianguo; Liu, Gang; Zhang, Jishuang; Ni, Kang; Zhu, Chunwu] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. [Chen, Chen; Jiang, Qian; Zhang, Jishuang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [Ziska, Lewis H.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Seneweera, Saman] Univ So Queensland, Ctr Syst Biol, Toowoomba, Qld 4350, Australia. RP Zhu, CW (reprint author), Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. EM cwzhu@issas.ac.cn FU National Basic Research Program (973 Program) [2014CB954500]; National Natural Science Foundation of China [31370457, 41301209, 31261140364, 31201126]; Chinese Academy of Sciences [KZCX3-SW-440]; Natural Science Foundation of Jiangsu Province in China [BK20131051, BK20140063] FX The work were supported by the National Basic Research Program (973 Program, 2014CB954500), the National Natural Science Foundation of China (grant nos. 31370457, 41301209, 31261140364, 31201126), the Knowledge Innovation Program of the Chinese Academy of Sciences (grant no. KZCX3-SW-440), and Natural Science Foundation of Jiangsu Province in China (grant no. BK20131051 and BK20140063). The FACE system instruments and apparatus were supplied by the National Institution of Agro-Environment Sciences and the Agricultural Research Center of Tohoku Region (Japan). NR 34 TC 2 Z9 3 U1 4 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD JUL PY 2015 VL 178 BP 63 EP 68 DI 10.1016/j.fcr.2015.03.023 PG 6 WC Agronomy SC Agriculture GA CK0GO UT WOS:000355883900008 ER PT J AU Meko, DM Friedman, JM Touchan, R Edmondson, JR Griffin, ER Scott, JA AF Meko, David M. Friedman, Jonathan M. Touchan, Ramzi Edmondson, Jesse R. Griffin, Eleanor R. Scott, Julian A. TI Alternative standardization approaches to improving streamflow reconstructions with ring-width indices of riparian trees SO HOLOCENE LA English DT Article DE age growth; cottonwood; dendrohydrology; detrending; "Little Ice Age'; Little Missouri River ID SEVERE SUSTAINED DROUGHT; MISSOURI RIVER; UNITED-STATES; GREAT-PLAINS; NORTH-DAKOTA; GROWTH; CLIMATE; TEMPERATURE; COTTONWOOD; REGIME AB Old, multi-aged populations of riparian trees provide an opportunity to improve reconstructions of streamflow. Here, ring widths of 394 plains cottonwood (Populus deltoides, ssp. monilifera) trees in the North Unit of Theodore Roosevelt National Park, North Dakota, are used to reconstruct streamflow along the Little Missouri River (LMR), North Dakota, US. Different versions of the cottonwood chronology are developed by (1) age-curve standardization (ACS), using age-stratified samples and a single estimated curve of ring width against estimated ring age, and (2) time-curve standardization (TCS), using a subset of longer ring-width series individually detrended with cubic smoothing splines of width against year. The cottonwood chronologies are combined with the first principal component of four upland conifer chronologies developed by conventional methods to investigate the possible value of riparian tree-ring chronologies for streamflow reconstruction of the LMR. Regression modeling indicates that the statistical signal for flow is stronger in the riparian cottonwood than in the upland chronologies. The flow signal from cottonwood complements rather than repeats the signal from upland conifers and is especially strong in young trees (e.g. 5-35years). Reconstructions using a combination of cottonwoods and upland conifers are found to explain more than 50% of the variance of LMR flow over a 1935-1990 calibration period and to yield reconstruction of flow to 1658. The low-frequency component of reconstructed flow is sensitive to the choice of standardization method for the cottonwood. In contrast to the TCS version, the ACS reconstruction features persistent low flows in the 19th century. Results demonstrate the value to streamflow reconstruction of riparian cottonwood and suggest that more studies are needed to exploit the low-frequency streamflow signal in densely sampled age-stratified stands of riparian trees. C1 [Meko, David M.; Touchan, Ramzi] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Friedman, Jonathan M.] US Geol Survey, Ft Collins Sci Ctr, Washington, DC USA. [Edmondson, Jesse R.] Univ Arkansas, Tree Ring Lab, Dept Geosci, Fayetteville, AR 72701 USA. [Griffin, Eleanor R.] US Geol Survey, Natl Res Program, Washington, DC USA. [Scott, Julian A.] US Forest Serv, Natl Stream & Aquat Ecol Ctr, USDA, Washington, DC USA. RP Meko, DM (reprint author), Univ Arizona, Tree Ring Res Lab, 1215 E Lowell St, Tucson, AZ 85721 USA. EM dmeko@LTRR.arizona.edu OI Friedman, Jonathan/0000-0002-1329-0663 FU National Park Service Climate Change Response Program, the National Park Service Water Resources Division; U.S. Geological Survey National Climate Change and Wildlife Science Center FX This work was supported by the National Park Service Climate Change Response Program, the National Park Service Water Resources Division, and the U.S. Geological Survey National Climate Change and Wildlife Science Center. NR 51 TC 4 Z9 4 U1 2 U2 19 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0959-6836 EI 1477-0911 J9 HOLOCENE JI Holocene PD JUL PY 2015 VL 25 IS 7 BP 1093 EP 1101 DI 10.1177/0959683615580181 PG 9 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CJ8NA UT WOS:000355758100004 ER PT J AU Yen, H Jeong, J Tseng, WH Kim, MK Records, RM Arabi, M AF Yen, Haw Jeong, Jaehak Tseng, Wen-Hsiao Kim, Min-Kyeong Records, Rosemary M. Arabi, Mazdak TI Computational Procedure for Evaluating Sampling Techniques on Watershed Model Calibration SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Optimization; Model evaluation; Behavior definition; Tree structured density estimation (TSDE) ID RAINFALL-RUNOFF MODELS; METROPOLIS ALGORITHM; GLOBAL OPTIMIZATION; PARAMETER-ESTIMATION; ASSESSMENT-TOOL; RANDOM SEARCH; INPUT DATA; UNCERTAINTY; SWAT; EVOLUTION AB A variety of computational techniques have been developed to efficiently and effectively draw realizations from the parameter space of watershed models for parameter estimation. However, to date, it has not been clearly understood how these techniques should be evaluated side by side. The main goal of this study is to develop and demonstrate a computational procedure for evaluating parameter sampling techniques. The analysis hinges on the evaluation of (1)efficiency in minimizing objective functions at the lowest required realizations; (2)effectiveness in drawing samples that adequately represent watershed characteristics based on automatic calibration results, and (3)effectiveness in enhancing the identifiability of the effective parameter space. The proposed procedure was applied to evaluate the performance of six commonly implemented sampling techniques for multisite, multiresponse parameter estimation of a river basin scale model in the Eagle Creek Watershed, Indiana, in the Midwestern United States. Results show that a particular technique surpassed all other methods in convergence speed and behavioral statistics. In addition, solutions derived using that technique were distributed closely in relatively small regions of the whole domain space, which enhanced the efficiency of the parameter search process. C1 [Yen, Haw; Jeong, Jaehak] Texas A&M AgriLife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. [Yen, Haw] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Tseng, Wen-Hsiao] Natl Cheng Kung Univ, Dept Hydraul & Ocean Engn, Tainan 701, Taiwan. [Kim, Min-Kyeong] Rural Dev Adm, Natl Acad Agr Sci, Suwon 441707, South Korea. [Records, Rosemary M.] Colorado State Univ, Dept Geosci, Ft Collins, CO USA. [Records, Rosemary M.; Arabi, Mazdak] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. RP Yen, H (reprint author), Texas A&M AgriLife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM hyen@brc.tamus.edu FU USDA-National Institute of Food and Agriculture Grants [2007-51130-03876, 2009-51130-06038]; Research Program for Agricultural Science AMP; Technology Development, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ008566] FX This study was supported in part by the USDA-National Institute of Food and Agriculture Grants 2007-51130-03876 and 2009-51130-06038 and the Research Program for Agricultural Science & Technology Development (Project No. PJ008566), National Academy of Agricultural Science, Rural Development Administration, Republic of Korea. Constructive comments by the associate editor and two anonymous reviewers greatly improved the quality of the paper. NR 61 TC 5 Z9 5 U1 2 U2 7 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD JUL PY 2015 VL 20 IS 7 AR UNSP 04014080 DI 10.1061/(ASCE)HE.1943-5584.0001095 PG 14 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA CK5CB UT WOS:000356238600010 ER PT J AU Anderson, MC Zolin, CA Hain, CR Semmens, K Yilmaz, MT Gao, F AF Anderson, Martha C. Zolin, Cornelio A. Hain, Christopher R. Semmens, Kathryn Yilmaz, M. Tugrul Gao, Feng TI Comparison of satellite-derived LAI and precipitation anomalies over Brazil with a thermal infrared-based Evaporative Stress Index for 2003-2013 SO JOURNAL OF HYDROLOGY LA English DT Article DE Evapotranspiration; Drought; Remote sensing; Brazil; Amazon ID MAPPING DAILY EVAPOTRANSPIRATION; SURFACE-ENERGY FLUXES; NORTHEAST BRAZIL; TIME-SERIES; DROUGHT; SCALES; AMAZON; MODEL; FORESTS; FIELD AB Shortwave vegetation index (VI) and leaf area index (LAI) remote sensing products yield inconsistent depictions of biophysical response to drought and pluvial events that have occurred in Brazil over the past decade. Conflicting reports of severity of drought impacts on vegetation health and functioning have been attributed to cloud and aerosol contamination of shortwave reflectance composites, particularly over the rainforested regions of the Amazon basin which are subject to prolonged periods of cloud cover and episodes of intense biomass burning. This study compares timeseries of satellite-derived maps of LAI from the Moderate Resolution Imaging Spectroradiometer (MODIS) and precipitation from the Tropical Rainfall Mapping Mission (TRMM) with a diagnostic Evaporative Stress Index (ES!) retrieved using thermal infrared remote sensing over South America for the period 2003-2013. This period includes several severe droughts and floods that occurred both over the Amazon and over unforested savanna and agricultural areas in Brazil. Cross-correlations between absolute values and standardized anomalies in monthly LAI and precipitation composites as well as the actual-to-reference evapotranspiration (ET) ratio used in the ESI were computed for representative forested and agricultural regions. The correlation analyses reveal strong apparent anticorrelation between MODIS LAI and TRMM precipitation anomalies over the Amazon, but better coupling over regions vegetated with shorter grass and crop canopies. The ESI was more consistently correlated with precipitation patterns over both landcover types. Temporal comparisons between ESI and TRMM anomalies suggest longer moisture buffering timescales in the deeper root. ed rainforest systems. Diagnostic thermal-based retrievals of ET and ET anomalies, such as used in the ES!, provide independent information on the impacts of extreme hydrologic events on vegetation health in comparison with VI and precipitation-based drought indicators, and used in concert may provide a more reliable evaluation of natural and managed ecosystem response to variable climate regimes. Published by Elsevier B.V. C1 [Anderson, Martha C.; Gao, Feng] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Zolin, Cornelio A.] Embrapa Agrosilvopastoral, Sinop, MT, Brazil. [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Semmens, Kathryn] Nat Nurture Ctr, Easton, PA USA. [Yilmaz, M. Tugrul] Middle E Tech Univ, Dept Civil Engn, Div Water Resources, TR-06531 Ankara, Turkey. RP Anderson, MC (reprint author), 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM martha.anderson@ars.usda.gov RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU Embrapa Visiting Scientist Program; Labex US; Brazilian Agricultural Research Corporation - Embrapa [10200.10/0215-9]; Agricultural Research Service - ARS FX This research was supported by funding from the Embrapa Visiting Scientist Program and from Labex US, an international scientific cooperation program sponsored by the Brazilian Agricultural Research Corporation - Embrapa, through Contract 10200.10/0215-9 with the Agricultural Research Service - ARS. NR 59 TC 10 Z9 10 U1 6 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD JUL PY 2015 VL 526 SI SI BP 287 EP 302 DI 10.1016/j.jhydrol.2015.01.005 PG 16 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CK0KS UT WOS:000355894700024 ER PT J AU Schaible, GD Mishra, AK Lambert, DM Panterov, G AF Schaible, Glenn D. Mishra, Ashok K. Lambert, Dayton M. Panterov, George TI Factors influencing environmental stewardship in US agriculture: Conservation program participants vs. non-participants SO LAND USE POLICY LA English DT Article DE Conservation practices; Conservation programs; Conservation policy; Environmental stewardship; Technology adoption; Cost-function models ID IRRIGATION TECHNOLOGY TRANSITIONS; COUNT DATA MODELS; SAMPLE SELECTION; ADOPTION; QUALITY; PROTECTION; TILLAGE; PLAINS; FARMER; STATES AB United States Department of Agriculture (USDA) conservation policy has increasingly shifted from a traditional land-retirement focus to greater emphasis on producer adoption of working-land conservation practices. This research made use of USDA integrated field/farm surveys, the Conservation Effects Assessment Project (CEAP) and Agricultural Resources Management Survey (ARMS), to (1) enhance understanding of operator, field, farm, economic, and environmental characteristic differences between conservation program participants and non-participants across a farm typology, and (2) to enhance understanding of the relative importance of these factors on influencing farm stewardship intensity in corn and wheat production, i.e., how these factors influence differences in producer adoption of alternative levels of land and pest-management practices between conservation program participants and non-participants. The research used a cost-function acreage-based technology adoption model to examine farm stewardship differences. Results indicate that program non-participants invest more heavily in land conserving and pest-management practices than program participants. Relative prices, structural, and socio-environmental factors play significantly different roles across crops, and between conservation program participants and non-participants, in their influence on producer adoption decisions for land and pest-management intensity. The environmental effectiveness and cost efficiency of conservation programs will likely improve when their implementation more explicitly recognizes farm heterogeneity as well as differences in farmer motivations for stewardship investments. Recognizing these differences can help improve targeting of conservation incentive structures. Published by Elsevier Ltd. C1 [Schaible, Glenn D.] USDA, Resource & Rural Econ Div, Econ Res Serv, Washington, DC 20250 USA. [Mishra, Ashok K.] Louisiana State Univ, Dept Agr Econ & Agribusiness, Baton Rouge, LA 70803 USA. [Lambert, Dayton M.] Univ Tennessee, Dept Agr & Resource Econ, Knoxville, TN 37996 USA. [Panterov, George] World Bank, Europe & Cent Asia, Washington, DC 20433 USA. RP Schaible, GD (reprint author), USDA, Resource & Rural Econ Div, Econ Res Serv, 1400 Independence Ave SW,Mail Stop 1800, Washington, DC 20250 USA. EM Schaible@ers.usda.gov; AMishra@agcenterr.LSU.edu; dmLambert@tennessee.edu; econgpanterov@gmail.com FU United States Department of Agriculture [TEN00442, LAB94163]; Tennessee Agricultural Experiment Station; Louisiana Agricultural Experiment Station FX Lambert's research was supported by United States Department of Agriculture Hatch Project TEN00442 and the Tennessee Agricultural Experiment Station. Mishra's time on this research was supported by United States Department of Agriculture Hatch Project LAB94163 and the Louisiana Agricultural Experiment Station. The authors appreciate the comments and suggestions from the Journal's two anonymous reviewers and from Marcel Aillery, Economic Research Service, U.S. Department of Agriculture. The views expressed are the author(s) and should not be attributed to the Economic Research Service, the U.S. Department of Agriculture, the University of Tennessee, Louisiana State University, or the World Bank. NR 54 TC 1 Z9 1 U1 5 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-8377 EI 1873-5754 J9 LAND USE POLICY JI Land Use Pol. PD JUL PY 2015 VL 46 BP 125 EP 141 DI 10.1016/j.landusepol.2015.01.018 PG 17 WC Environmental Studies SC Environmental Sciences & Ecology GA CK3IT UT WOS:000356110500011 ER PT J AU Weed, AS Bentz, BJ Ayres, MP Holmes, TP AF Weed, Aaron S. Bentz, Barbara J. Ayres, Matthew P. Holmes, Thomas P. TI Geographically variable response of Dendroctonus ponderosae to winter warming in the western United States SO LANDSCAPE ECOLOGY LA English DT Article DE Climate change; Demography; Mountain pine beetle; Process-based model; Bark beetles; Pinus; Cold tolerance ID MOUNTAIN PINE-BEETLE; POPULATION-GROWTH RATES; CLIMATE-CHANGE; RANGE EXPANSION; BARK BEETLE; SPATIOTEMPORAL PATTERNS; BRITISH-COLUMBIA; TREE MORTALITY; NORTH-AMERICA; SPATIAL SYNCHRONY AB Milder winters have contributed to recent outbreaks of Dendroctonus ponderosae in Canada, but have not been evaluated as a factor permitting concurrent outbreaks across its large range (ca.1500 x 1500 km) in the western United States (US). We examined the trend in minimum air temperatures in D. ponderosae habitats across the western US and assessed whether warming winters explained the occurrence of outbreaks using physiological and population models. We used climate data to analyze the history of minimum air temperatures and reconstruct physiological effects of cold on D. ponderosae. We evaluated relations between winter temperatures and beetle abundance using aerial detection survey data. Extreme winter temperatures have warmed by about 4 A degrees C since 1960 across the western US. At the broadest scale, D. ponderosae population dynamics between 1997 and 2010 were unrelated to variation in minimum temperatures, but relations between cold and D. ponderosae dynamics varied among regions. In the 11 coldest ecoregions, lethal winter temperatures have become less frequent since the 1980s and beetle-caused tree mortality increased-consistent with the climatic release hypothesis. However, in the 12 warmer regions, recent epidemics cannot be attributed to warming winters because earlier winters were not cold enough to kill D. ponderosae. There has been pronounced warming of winter temperatures throughout the western US, and this has reduced previous constraints on D. ponderosae abundance in some regions. However, other considerations are necessary to understand the broad extent of recent D. ponderosae epidemics in the western US. C1 [Weed, Aaron S.; Ayres, Matthew P.] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Bentz, Barbara J.] US Forest Serv, USDA, Rocky Mt Res Stn, Logan, UT 84321 USA. [Holmes, Thomas P.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC 27709 USA. RP Weed, AS (reprint author), Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. EM aaron_weed@nps.gov FU Southern Research Station (USDA Forest Service) FX We thank Jim Vandygriff for querying damage data from the main ADS database and Jacques Regniere for the BioSIM temperature database. Helpful comments were provided from Sandy Liebhold. Funding was provided by a cooperative agreement with the Southern Research Station (USDA Forest Service) to M.P.A. NR 96 TC 8 Z9 9 U1 5 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD JUL PY 2015 VL 30 IS 6 BP 1075 EP 1093 DI 10.1007/s10980-015-0170-z PG 19 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CJ7EM UT WOS:000355657200009 ER PT J AU DeJonge, KC Taghvaeian, S Trout, TJ Comas, LH AF DeJonge, Kendall C. Taghvaeian, Saleh Trout, Thomas J. Comas, Louise H. TI Comparison of canopy temperature-based water stress indices for maize SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Infrared thermometry; Crop water stress index (CWSI); Degrees above non-stressed (DANS); Deficit irrigation; Soil water deficit ID INFRARED THERMOMETRY; DEFICIT IRRIGATION; LIMITED IRRIGATION; THRESHOLD METHOD; TIME-THRESHOLD; ENERGY-BALANCE; GRAIN-SORGHUM; SOIL DROUGHT; CORN; PLANT AB Infrared thermal radiometers (IRTs) are an affordable tool for researchers to monitor canopy temperature. In this maize experiment, six treatments of regulated deficit irrigation levels were evaluated. The main objective was to evaluate these six treatments in terms of six indices (three previously proposed and three introduced in this study) used to quantify water stress. Three are point-in-time indices where one daily reading is assumed representative of the day (Crop Water Stress Index - CWSI, Degrees Above Non-Stressed DANS, Degrees Above Canopy Threshold - DACT) and three integrate the cumulative impact of water stress over time (Time Temperature Threshold - TTT, Integrated Degrees Above Non-Stressed - IDANS, Integrated Degrees Above Canopy Threshold - IDACT). Canopy temperature was highly correlated with leaf water potential (R-2=0.895). To avoid potential bias, the lowest observation from the non-stressed treatment was chosen as the baseline for DANS and IDANS indices. Early afternoon temperatures showed the most divergence and thus this is the ideal time to obtain spot index values. Canopy temperatures and stress indices were responsive to evapotranspiration-based irrigation treatments. DANS and DACT were highly correlated with CWSI above the corn threshold 28 degrees C used in the TIT method, and all indices showed linear relationship with soil water deficit at high temperatures. Recommendations are given to consider soils with high water-holding capacity when choosing a site for non-stressed reference crops used in the DANS method. The DACT may be the most convenient index, as all it requires is a single canopy temperature measurement yet has strong relationships with other indices and crop water measurements. Published by Elsevier B.V. C1 [DeJonge, Kendall C.; Trout, Thomas J.; Comas, Louise H.] USDA ARS, Water Management Res Unit, Ft Collins, CO 80522 USA. [Taghvaeian, Saleh] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. RP DeJonge, KC (reprint author), USDA ARS, Water Management Res Unit, 2150 Ctr Ave,Bldg D Ste 320, Ft Collins, CO 80522 USA. EM kendall.dejonge@ars.usda.gov; saleh.taghvaeian@okstate.edu; thomas.trout@ars.usda.gov; louise.comas@ars.usda.gov OI Trout, Thomas/0000-0003-1896-9170 NR 52 TC 8 Z9 8 U1 5 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JUL 1 PY 2015 VL 156 BP 51 EP 62 DI 10.1016/j.agwat.2015.03.023 PG 12 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CJ7WY UT WOS:000355712300006 ER PT J AU Webb, KM Harveson, RM West, MS AF Webb, K. M. Harveson, R. M. West, M. S. TI Evaluation of Rhizoctonia zeae as a potential biological control option for fungal root diseases of sugar beet SO ANNALS OF APPLIED BIOLOGY LA English DT Article DE Antagonistic fungi; Beta vulgaris; soilborne pathogens; sugarbeet ID CERCOSPORA LEAF-SPOT; CENTRAL HIGH-PLAINS; FUSARIUM-OXYSPORUM; LAETISARIA-ARVALIS; WAITEA-CIRCINATA; PLANT-PATHOGENS; CONTROL AGENT; DAMPING-OFF; SOLANI; SOIL AB Several common root diseases routinely damage sugar beet in Nebraska and other production areas of the Central High Plains, and it is becoming more common to find fields infested simultaneously with multiple pathogens. Owing to the shortage of available fungicides for economic management of soilborne diseases, alternative techniques such as biological control are increasingly being sought for disease management. Over the last several years, unidentified, sterile fungi have been isolated in conjunction with pathogens from infected sugar beet roots and seedlings. At least two promising isolates have been identified from in vitro assays that inhibit the radial growth of multiple sugar beet root pathogens, including Rhizoctonia solani, Fusarium oxysporum f. sp. betae, Phoma betae and Pythium aphanidermatum. Based on morphological and molecular characterisation, two isolates, Hall' and R47', were putatively identified as Rhizoctonia zeae. In vitro pathogenicity testing indicated that these isolates were not pathogens of sugar beet. Both isolates were compared with the well-established biological control fungus Laetisaria arvalis and tested as potential treatments in a field naturally infested with multiple sugar beet root diseases. Data indicated that these fungi provided some level of protection against a complex of soilborne diseases and suggest that these isolates could have a potential fit in an integrative management strategy for several sugar beet root diseases. C1 [Webb, K. M.] USDA ARS, Sugar Beet Res Unit, Ft Collins, CO 80526 USA. [Harveson, R. M.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE USA. [West, M. S.] USDA ARS, Plains Area Off, Ft Collins, CO 80526 USA. RP Webb, KM (reprint author), USDA ARS, Sugar Beet Res Unit, Ft Collins, CO 80526 USA. EM kimberly.webb@ars.usda.gov NR 77 TC 1 Z9 1 U1 5 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0003-4746 EI 1744-7348 J9 ANN APPL BIOL JI Ann. Appl. Biol. PD JUL PY 2015 VL 167 IS 1 BP 75 EP 89 DI 10.1111/aab.12210 PG 15 WC Agriculture, Multidisciplinary SC Agriculture GA CJ7TT UT WOS:000355703300007 ER PT J AU Whitehead, TR Johnson, CN Patel, NB Cotta, MA Moore, ERB Lawson, PA AF Whitehead, Terence R. Johnson, Crystal N. Patel, Nisha B. Cotta, Michael A. Moore, Edward R. B. Lawson, Paul A. TI Savagea faecisuis gen. nov., sp nov., a tylosin- and tetracycline-resistant bacterium isolated from a swine-manure storage pit SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Savagea; 16S rRNA; Phylogeny; Taxonomy; Manure; Swine ID COMB. NOV; ET-AL.; EMENDED DESCRIPTION; CLINICAL SPECIMENS; BACILLUS-SUBTILIS; LIPID-COMPOSITION; RECLASSIFICATION; PROPOSAL; IDENTIFICATION; FECES AB A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular methods was performed on three strains of a Gram-stain positive, non-sporeforming, motile aerobic rod-shaped bacterium resistant to tylosin and tetracycline isolated from a swine-manure storage pit. On the basis of 16S rRNA gene sequence analyses, it was confirmed that these isolates are highly related to each other and form a hitherto unknown lineage within the Planococcaceae. In particular, pairwise analysis of the 16S rRNA gene sequence demonstrated that the novel organism is closely related to members of the genus Sporosarcina (92.8-94.5 %), Pyschrobacillus (93.5-93.9 %) and Paenisporosarcina (93.3-94.5 %). The predominant fatty acids were found to consist of iso-C-15:0 and iso-C-17:1 omega 10c and the G+C mol% was determined to be 41.8. Based on biochemical, chemotaxonomic, and phylogenetic evidence, it is proposed that these novel strains be classified as a novel genus and species, Savagea faecisuis gen nov., sp. nov. The type strain is Con12(T) (=CCUG 63563(T) = NRRL B-59945(T) = NBRC 109956(T)). C1 [Whitehead, Terence R.; Cotta, Michael A.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Johnson, Crystal N.; Patel, Nisha B.; Lawson, Paul A.] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Moore, Edward R. B.] Univ Gothenburg, Dept Clin Bacteriol, Culture Collect, S-41346 Gothenburg, Sweden. [Lawson, Paul A.] Univ Oklahoma, Ecol & Evolutionary Biol Program, Norman, OK 73019 USA. RP Whitehead, TR (reprint author), ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Terry.Whitehead@ars.usda.gov; paul.lawson@ou.edu OI Patel, Nisha/0000-0003-2337-9984 NR 50 TC 1 Z9 1 U1 1 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD JUL PY 2015 VL 108 IS 1 BP 151 EP 161 DI 10.1007/s10482-015-0473-8 PG 11 WC Microbiology SC Microbiology GA CJ8KE UT WOS:000355748200015 PM 25980832 ER PT J AU Moore, MT Pierce, JR Farris, JL AF Moore, Matthew T. Pierce, Jon R. Farris, Jerry L. TI Water-Quality Analysis of an Intensively Used On-Farm Storage Reservoir in the Northeast Arkansas Delta SO ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article ID WASTE-WATER; REUSE; RUNOFF AB The use of farm reservoirs for supplemental irrigation is gaining popularity in the Mississippi Alluvial Plain (MAP). Due to depletions of several aquifers, many counties within the MAP have been designated as critical-use groundwater areas. To help alleviate stress on these aquifers, many farmers are implementing storage reservoirs for economic and conservation benefits. When used in tandem with a tailwater recovery system, reservoirs have the potential to trap and transform potential contaminants (e.g., nutrients and pesticides) rather than releasing them through drainage into receiving systems such as lakes, rivers, and streams. Roberts Reservoir is an intensively used, 49-ha on-farm storage reservoir located in Poinsett County, Arkansas. Water-quality analyses and toxicity assessments of the reservoir and surrounding ditches indicated a stable water-quality environment with no observed toxicity present in collected samples. Results of this study suggest that water released into a local receiving stream poses no contaminant risk and could be maintained for irrigation purposes, thereby decreasing the need for additional groundwater depletion. C1 [Moore, Matthew T.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Pierce, Jon R.; Farris, Jerry L.] Arkansas State Univ, Dept Biol Sci, State Univ, AR 72467 USA. RP Moore, MT (reprint author), USDA ARS, Natl Sedimentat Lab, POB 1157, Oxford, MS 38655 USA. EM matt.moore@ars.usda.gov NR 28 TC 1 Z9 1 U1 2 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0090-4341 EI 1432-0703 J9 ARCH ENVIRON CON TOX JI Arch. Environ. Contam. Toxicol. PD JUL PY 2015 VL 69 IS 1 BP 89 EP 94 DI 10.1007/s00244-015-0158-3 PG 6 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA CJ7OJ UT WOS:000355685600010 PM 25912809 ER PT J AU Yemshanov, D Koch, FH Riitters, KH McConkey, B Huffman, T Smith, S AF Yemshanov, Denys Koch, Frank H. Riitters, Kurt H. McConkey, Brian Huffman, Ted Smith, Stephen TI Assessing land clearing potential in the Canadian agriculture-forestry interface with a multi-attribute frontier approach SO ECOLOGICAL INDICATORS LA English DT Article DE Multi-attribute frontier; Non-dominance; Land clearing potential; Agriculture-forestry interface; Canadian land inventory; Landscape fragmentation potential ID MULTICRITERIA DECISION-ANALYSIS; ECOLOGICAL RISK-ASSESSMENT; CENTRAL SASKATCHEWAN; PORTFOLIO THEORY; DEFORESTATION; MANAGEMENT; UNCERTAINTY; DIVERSIFICATION; LANDSCAPES; SATELLITE AB The pattern of forest land clearing in a region can be viewed as a gauge of sustainable (or unsustainable) use of agricultural and forest resources. In this study we examine the geographical distribution of land clearing potential in the Canadian agriculture forestry interface and propose a new landscape-scale indicator that quantifies this potential. We consider the possibility that forested land will be cleared for agriculture as a trade-off between the land's capacity to support agriculture and its comparative value if it remains forested. However, this trade-off is complicated by the land's susceptibility to fragmentation (and subsequent conversion), which derives from the local pattern of forest, agriculture and other land cover types. We find the locations in the agriculture forestry interface with the highest land clearing potential by delineating nested multi-attribute frontiers in the dimensions of the land's agricultural capacity, its estimated forest productivity and its fragmentation potential. The multi-attribute frontier concept addresses our lack of knowledge about the relative importance of these multiple drivers of land conversion by objectively combining them into a single-dimensional land clearing pressure metric in a geographical setting. Overall, our approach provides a simple yet informative indicator which reveals the geographical stratification of land clearing pressures across large regions. In general, the spatial delineation of areas with high land clearing potential agrees well with recent evidence of land clearing and deforestation events in Canada. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved. C1 [Yemshanov, Denys] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Koch, Frank H.; Riitters, Kurt H.] US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, Res Triangle Pk, NC 27709 USA. [McConkey, Brian] Agr & Agri Food Canada, Swift Current, SK S9H 3X2, Canada. [Huffman, Ted] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada. [Smith, Stephen] Agr & Agri Food Canada, Res & Anal Directorate, Ottawa, ON K1A 0C5, Canada. RP Yemshanov, D (reprint author), Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM dyemshan@nrcan.gc.ca; fhkoch@fs.fed.us; kriitters@fs.fed.us; brian.mcconkey@agr.gc.ca; ted.huffman@agr.gc.ca; stephen.smith@agr.gc.ca OI Koch, Frank/0000-0002-3750-4507 FU Natural Resources Canada OERD FX The participation of Denys Yemshanov, Brian McConkey, Ted Huffman and Steven Smith was supported by Natural Resources Canada OERD Phase-III project "Maximizing environmental benefits of the bioeconomy using agricultural feedstock". NR 84 TC 0 Z9 0 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD JUL PY 2015 VL 54 BP 71 EP 81 DI 10.1016/j.ecolind.2015.02.019 PG 11 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CJ3EF UT WOS:000355365500007 ER PT J AU Simoncic, T Spies, TA Deal, RL Boncina, A AF Simoncic, Tina Spies, Thomas A. Deal, Robert L. Boncina, Andrej TI A Conceptual Framework for Characterizing Forest Areas with High Societal Values: Experiences from the Pacific Northwest of USA and Central Europe SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Priority area; Allocation; Forest planning; Ecosystem services; Spatially explicit approaches; Segregation versus integration forest management ID PROTECTION FORESTS; ECOSYSTEM SERVICES; FEDERAL FORESTS; UNITED-STATES; SUSTAINABLE MANAGEMENT; RESTORATION FRAMEWORK; POLICY; BIODIVERSITY; PLAN; CONSERVATION AB In recent decades, much work has been invested to describe forest allocations with high societal values. Yet, few comparative analyses have been conducted on their importance and differences across the regions of the globe. This paper introduces a conceptual framework to characterize forest priority areas defined as areas with identified higher importance of societal values in the context of multi-objective forest management. The six dimensions of the framework (designation objective, prioritization of objectives, governance, permanency, spatial scale, and management regime) characterize the general approach (integrative vs. segregative) to multi-objective forest management and explain the form and role of priority areas for providing forest services. The framework was applied in two case study regions-Pacific Northwest of USA (PNW) and Central Europe (CE). Differences between the regions exist in all dimensions. Late-successional and riparian reserves are specific to the PNW, while protection against natural hazards is specific to CE. In PNW, priority areas are mainly focused on public lands whereas in CE they include public and private lands. Priority areas in PNW are designated in a much larger spatial context and have longer time commitments. In CE, integration of management objectives on priority areas prevails, whereas in PNW priority areas tend to be designated for single objectives. In CE, greater tolerance of timber management within priority areas compared to PNW is allowed. Convergent trends in application of priority areas between the regions indicate mixing of segregation and integration approaches to forest management. C1 [Simoncic, Tina; Boncina, Andrej] Univ Ljubljana, Dept Forestry & Renewable Forest Resources, Biotehn Fac, Ljubljana 1000, Slovenia. [Spies, Thomas A.] US Forest Serv, USDA, PNW Res Stn, Corvallis, OR 97331 USA. [Deal, Robert L.] US Forest Serv, USDA, PNW Res Stn, Portland, OR 97331 USA. RP Simoncic, T (reprint author), Univ Ljubljana, Dept Forestry & Renewable Forest Resources, Biotehn Fac, Vecna Pot 83, Ljubljana 1000, Slovenia. EM tina.simoncic@bf.uni-lj.si; tspies@fs.fed.us; rdeal@fs.fed.us; andrej.boncina@bf.uni-lj.si FU Pahernik Foundation FX The authors wish to thank Christian Rosset and Tom Nygren for providing reviews of an early draft. We would also like to thank Pahernik Foundation for supporting the publishing of results. NR 108 TC 2 Z9 2 U1 2 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD JUL PY 2015 VL 56 IS 1 BP 127 EP 143 DI 10.1007/s00267-015-0482-4 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA CJ6RL UT WOS:000355621100010 PM 25894271 ER PT J AU Aamot, HU Ward, TJ Brodal, G Vralstad, T Larsen, GB Klemsdal, SS Elameen, A Uhlig, S Hofgaard, IS AF Aamot, H. U. Ward, T. J. Brodal, G. Vralstad, T. Larsen, G. B. Klemsdal, S. S. Elameen, A. Uhlig, S. Hofgaard, I. S. TI Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE 3-ADON; 15-ADON; Aggressiveness; Growth rate; Population ID HEAD BLIGHT PATHOGEN; NIVALENOL-PRODUCING CHEMOTYPES; GIBBERELLA-ZEAE; F-GRAMINEARUM; GENEALOGICAL CONCORDANCE; SENSU-STRICTO; FUMONISIN B-1; WINTER-WHEAT; DEOXYNIVALENOL; POPULATIONS AB As has been observed in several European countries, the frequency of Fusarium head blight (FHB) caused by members of the Fusarium graminearum species complex (FGSC) has increased in Norwegian cereals in recent years, resulting in elevated levels of deoxynivalenol in cereal grains. The objective of this study was to determine if this increase was associated with changes in FGSC composition within Norway. FGSC isolates collected from wheat, oats and barley in Norway during two periods, mainly 1993-1998 and 2004-2007, were characterized to determine species and trichothecene genotype composition and to assess levels of genetic variation and population structure. In vitro growth rates at different temperatures and aggressiveness in spring wheat were further characterized for a sub-selection of isolates. All Norwegian isolates were identified as F. graminearum. The 3-acetyl-deoxynivalenol (3-ADON) trichothecene type was dominant. However, isolates with the 15-ADON chemotype were detected in Norway for the first time and may represent a recent introduction of this trichothecene type. Bayesian-model based clustering and analyses of genetic differentiation indicated the persistence over the last 20 years of two sympatric and partially admixed populations of F. graminearum in Norway. Significant differences in average in vitro growth rates and aggressiveness were observed between these two populations. Our results demonstrate that the recent increase in prevalence of the FGSC in Norwegian cereals do not correspond to any dramatic changes in FGSC species or trichothecene chemotype composition. However, significant changes in population frequencies were observed among Norwegian F. graminearum. C1 [Aamot, H. U.; Brodal, G.; Larsen, G. B.; Klemsdal, S. S.; Elameen, A.; Hofgaard, I. S.] Norwegian Inst Agr & Environm Res, Bioforsk, N-1430 As, Norway. [Ward, T. J.] ARS, USDA, Peoria, IL 61604 USA. [Vralstad, T.; Uhlig, S.] Norwegian Vet Inst, N-0106 Oslo, Norway. RP Hofgaard, IS (reprint author), Norwegian Inst Agr & Environm Res, Bioforsk, Hogskoleveien 7, N-1430 As, Norway. EM ingerd.hofgaard@bioforsk.no FU Foundation for Research Levy on Agricultural Products/Agricultural Agreement Research Fund/the Research Council of Norway [173277/I10, 199412/I99]; Norwegian cereal industry and Bioforsk FX This work was funded by the following partners: the Foundation for Research Levy on Agricultural Products/Agricultural Agreement Research Fund/the Research Council of Norway (research grant 173277/I10 and 199412/I99), the Norwegian cereal industry and Bioforsk. Isolates of F. graminearum were kindly provided by Ludwig Niessen, Technical University of Munich Germany; Paul Nicolson, John Innes Centre, Norwich, UK, and Tapani Yli-Mattila, University of Turku, Finland. We are grateful to Tom Usgaard and Nathane Orwig for excellent technical assistance. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. NR 79 TC 8 Z9 8 U1 4 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2015 VL 142 IS 3 BP 501 EP 519 DI 10.1007/s10658-015-0629-4 PG 19 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CJ6RW UT WOS:000355622300007 ER PT J AU Xu, ZY Yu, J Kohel, RJ Percy, RG Beavis, WD Main, D Yu, JZ AF Xu, Zhanyou Yu, Jing Kohel, Russell J. Percy, Richard G. Beavis, William D. Main, Dorrie Yu, John Z. TI Distribution and evolution of cotton fiber development genes in the fibreless Gossypium raimondii genome SO GENOMICS LA English DT Article DE Cotton (Gossypium spp.); Fiber development; EST Unigenes; Recombination hotspots; Gene distribution; Genome evolution ID ALLOTETRAPLOID COTTON; TETRAPLOID COTTON; WIDE ANALYSIS; HIRSUTUM L.; MAP; EXPRESSION; SEQUENCE; CELL; REVEALS; ORGANIZATION AB Cotton fiber represents the largest single cell in plants and they serve as models to study cell development. This study investigated the distribution and evolution of fiber Unigenes anchored to recombination hotspots between tetraploid cotton (Gossypium hirsutum) At and Dt subgenomes, and within a parental diploid cotton (Gossypium raimondii) D genome. Comparative analysis of At vs D and Dt vs D showed that 1) the D genome provides many fiber genes after its merger with another parental diploid cotton (Gossypium arboreum) A genome although the D genome itself does not produce any spinnable fiber; 2) similarity of fiber genes is higher between At vs D than between Dt vs D genomic hotspots. This is the first report that fiber genes have higher similarity between At and D than between Dt and D. The finding provides new insights into cotton genomic regions that would facilitate genetic improvement of natural fiber properties. Published by Elsevier Inc. C1 [Xu, Zhanyou; Yu, Jing; Kohel, Russell J.; Percy, Richard G.; Yu, John Z.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Xu, Zhanyou; Beavis, William D.] Iowa State Univ, Bioinformat & Computat Biol, Ames, IA USA. [Yu, Jing; Main, Dorrie] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. RP Yu, JZ (reprint author), USDA ARS, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. EM john.yu@ars.usda.gov FU USDA-ARS CRIS Project [3091-21000-038-00D] FX The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint first authors. This research was supported by USDA-ARS CRIS Project 3091-21000-038-00D. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The U.S. Department of Agriculture is equal opportunity provider and employer. NR 45 TC 1 Z9 1 U1 2 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 EI 1089-8646 J9 GENOMICS JI Genomics PD JUL PY 2015 VL 106 IS 1 BP 61 EP 69 DI 10.1016/j.ygeno.2015.03.002 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CJ7KW UT WOS:000355674900008 PM 25796538 ER PT J AU Evangelista, RL Hojilla-Evangelista, MP Cermak, SC Isbell, TA AF Evangelista, Roque L. Hojilla-Evangelista, Milagros P. Cermak, Steven C. Isbell, Terry A. TI Dehulling of coriander fruit before oil extraction SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Coriander; Dehulling; Coriander oil; Oil extraction ID SEED OILS; PHYSICAL-PROPERTIES; PETROSELINIC ACID; SATIVUM L.; CANOLA AB Coriander (Coriandrum sativum L.) is a summer annual traditionally grown for use as fresh green herb, spice or for its essential oil. The essential oil is obtained by steam distillation of crushed fruit and the residue is utilized as feed or processed further to recover the triglyceride. The triglyceride is obtained from the whole fruit by mechanical pressing or by solvent extraction. The coriander fruit has low density due to the void space between the mericarps and the high proportion of hulls in the fruit's weight. Dehulling oilseeds before oil extraction is commonly employed whenever it is practical to do so, but this technique has not been applied to coriander seeds. This study evaluated the feasibility of dehulling coriander fruits as part of the seed preparation before oil extraction and developed a simple process of dehulling coriander. Coriander fruit with 17.6% oil and 10% moisture was dehulled by first splitting the fruit using an impact huller or a roller mill. Seeds still encased in the split hulls were loosened further using the impact huller. The hulls were removed by screening, aspirating, or using a gravity separator. Dehulled seed with purity >95% was attainable. Dehulling the coriander fruit reduced the weight by 50% and reduced the volume by 60%. Dehulling coriander also doubled the oil content, increased the crude protein by 70%, reduced the crude fiber by 28%, and reduced other carbohydrates by 65%. The coriander fruit and dehulled seeds with purity up to 95% were amenable to screw pressing. Prepressing and solvent extraction would be more appropriate for pure dehulled seeds due to their high oil content. Dehulled seeds must be oil extracted right away to minimize the increase in free fatty acid. Published by Elsevier B.V. C1 [Evangelista, Roque L.; Cermak, Steven C.; Isbell, Terry A.] ARS, Biooils Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Hojilla-Evangelista, Milagros P.] ARS, Plant Polymer Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Evangelista, RL (reprint author), ARS, Biooils Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Roque.Evangelista@ars.usda.gov NR 35 TC 3 Z9 3 U1 1 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD JUL PY 2015 VL 69 BP 378 EP 384 DI 10.1016/j.indcrop.2015.02.057 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA CJ3ED UT WOS:000355365300050 ER PT J AU Sanchez, NCB Carroll, JA Donaldson, JR Buntyn, JO Schmidt, TB AF Sanchez, Nicole C. Burdick Carroll, Jeffery A. Donaldson, Janet R. Buntyn, Joe O. Schmidt, Ty B. TI Exogenous administration of lipids to steers alters aspects of the innate immune response to endotoxin challenge SO INNATE IMMUNITY LA English DT Article DE cattle; glucose; innate immunity; non-esterified fatty acids; temperament ID NONESTERIFIED FATTY-ACIDS; RECTAL TEMPERATURE; TECHNICAL NOTE; BRAHMAN BULLS; BLOOD-GLUCOSE; IN-VIVO; TEMPERAMENT; HUMANS; INFUSION; CORTISOL AB This study examined the effects of increasing energy availability from both dextrose and lipid treatments on the pro-inflammatory response to LPS in Holstein steers. Steers were randomly assigned to one of three groups: saline at 0.5ml/kg body weight (Control) or 50% dextrose [0.5ml/kg body weight (Dextrose) to mimic calm cattle's response to LPS] administered immediately prior to LPS (0.5 mu g/kg body weight at 0h) or continuous lipid emulsion infusion from -1 to 6h [Intralipid 20% (Baxter, Deerfield, IL USA); 0.5ml/kg/hr (Lipid) to mimic temperamental cattle]. Concentrations of non-esterified fatty acids (NEFA) were greater in Lipid compared with Control and Dextrose steers. A greater decrease in the change in rectal temperature, relative to baseline, was observed in response to LPS in Dextrose in comparison to control and Lipid steers. Cortisol was greater in Lipid than Dextrose and Control steers from -0.5 to 0h, yet decreased from 0.5 to 5.5h relative to LPS challenge. Concentrations of IL-6 were decreased in Lipid steers compared with Dextrose and Control steers, and were decreased in Dextrose compared with Control steers post-LPS challenge. These data suggest that increasing circulating NEFA using an exogenous Lipid emulsion may modulate the pro-inflammatory response in steers. C1 [Sanchez, Nicole C. Burdick; Carroll, Jeffery A.] ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Donaldson, Janet R.] Mississippi State Univ, Dept Biol Sci, Mississippi State, MS 39762 USA. [Buntyn, Joe O.; Schmidt, Ty B.] Univ Nebraska, Dept Anim Sci, Lincoln, NE USA. RP Carroll, JA (reprint author), ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. EM jeff.carroll@ars.usda.gov NR 26 TC 2 Z9 2 U1 0 U2 3 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1753-4259 EI 1753-4267 J9 INNATE IMMUN-LONDON JI Innate Immun. PD JUL PY 2015 VL 21 IS 5 BP 512 EP 522 DI 10.1177/1753425914552741 PG 11 WC Biochemistry & Molecular Biology; Immunology; Medicine, Research & Experimental; Microbiology SC Biochemistry & Molecular Biology; Immunology; Research & Experimental Medicine; Microbiology GA CJ6RA UT WOS:000355619800006 PM 25305381 ER PT J AU Prabhakara, K Hively, WD McCarty, GW AF Prabhakara, Kusuma Hively, W. Dean McCarty, Gregory W. TI Evaluating the relationship between biomass, percent groundcover and remote sensing indices across six winter cover crop fields in Maryland, United States SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Winter cover crops; Biomass; Percent groundcover; Remote sensing; Vegetation indices ID HYPERSPECTRAL VEGETATION INDEXES; CHESAPEAKE BAY; WATER-QUALITY; GREEN; WHEAT; AGRICULTURE; ALGORITHMS; SOIL; RED AB Winter cover crops are an essential part of managing nutrient and sediment losses from agricultural lands. Cover crops lessen sedimentation by reducing erosion, and the accumulation of nitrogen in aboveground biomass results in reduced nutrient runoff. Winter cover crops are planted in the fall and are usually terminated in early spring, making them susceptible to senescence, frost burn, and leaf yellowing due to wintertime conditions. This study sought to determine to what extent remote sensing indices are capable of accurately estimating the percent groundcover and biomass of winter cover crops, and to analyze under what critical ranges these relationships are strong and under which conditions they break down. Cover crop growth on six fields planted to barley, rye, ryegrass, triticale or wheat was measured over the 2012-2013 winter growing season. Data collection included spectral reflectance measurements, aboveground biomass, and percent groundcover. Ten vegetation indices were evaluated using surface reflectance data from a 16-band CROPSCAN sensor. Restricting analysis to sampling dates before the onset of prolonged freezing temperatures and leaf yellowing resulted in increased estimation accuracy. There was a strong relationship between the normalized difference vegetation index (NDVI) and percent groundcover (r(2) = 0.93) suggesting that date restrictions effectively eliminate yellowing vegetation from analysis. The triangular vegetation index (TVI) was most accurate in estimating high ranges of biomass (r(2) = 0.86), while NDVI did not experience a clustering of values in the low and medium biomass ranges but saturated in the higher range (>1500 kg/ha). The results of this study show that accounting for index saturation, senescence, and frost burn on leaves can greatly increase the accuracy of estimates of percent groundcover and biomass for winter cover crops. Published by Elsevier B.V. C1 [Prabhakara, Kusuma] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Hively, W. Dean] US Geol Survey, Eastern Geog Sci Ctr, Reston, VA 22092 USA. [McCarty, Gregory W.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Prabhakara, K (reprint author), Univ Maryland, Dept Geog Sci, 2181 Samuel J LeFrak Hall, College Pk, MD 20742 USA. EM kusumaprabhak@gmail.com FU USDA Choptank River Conservation Effects Assessment Project (CEAP); USGS Land Change Science Program; National Fish and Wildlife Foundation FX Thank you to Ms. Megan Parry, Mr. Mouhamad Diabate, and Mr. Antonio Pereira for help with fieldwork and in the laboratory. Additional thanks to Dr. Chris Justice, Dr. Samuel Coward, and Dr. Ralph Dubayah for their guidance on this study. This research was a collaboration between the USDA-ARS Hydrology and Remote Sensing Laboratory, the USGS Eastern Geographic Science Center, and the University of Maryland, College Park. Funding was provided by the USDA Choptank River Conservation Effects Assessment Project (CEAP), the USGS Land Change Science Program, and the National Fish and Wildlife Foundation. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 37 TC 7 Z9 7 U1 9 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD JUL PY 2015 VL 39 BP 88 EP 102 DI 10.1016/j.jag.2015.03.002 PG 15 WC Remote Sensing SC Remote Sensing GA CJ3FD UT WOS:000355367900011 ER PT J AU Szogi, AA Vanotti, MB Hunt, PG AF Szoegi, Ariel A. Vanotti, Matias B. Hunt, Patrick G. TI Phosphorus recovery from pig manure solids prior to land application SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Phosphorus; Nitrogen; N:P ratio; Pig manure; Nutrient recovery; Animal waste ID POULTRY LITTER; QUALITY; IMBALANCES; PHOSPHATE; NITROGEN; WASTE AB Land disposal of pig manure is an environmental concern due to an imbalance of the nitrogen to phosphorus (N:P) ratio for crop production, leading to excess phosphorus (P) in soils and potential risks of water pollution. A process called "quick wash" was investigated for its feasibility to extract and recover P from pig manure solids. This process consists of selective dissolution of P from solid manure into a liquid extract using mineral or organic acid solutions, and recovery of P from the liquid extract by adding lime and an organic polymer to form a P precipitate. Laboratory tests confirmed the quick wash process selectively removed and recovered up to 90% of the total (TP) from fresh pig manure solids while leaving significant amounts of nitrogen (N) in the washed manure residue. As a result of manure P extraction, the washed solid residue became environmentally safer for land application with a more balanced N:P ratio for crop production. The recovered P can be recycled and used as fertilizer for crop production while minimizing manure P losses into the environment. Published by Elsevier Ltd. C1 [Szoegi, Ariel A.; Vanotti, Matias B.; Hunt, Patrick G.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Szogi, AA (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM ariel.szogi@ars.usda.gov FU USDA-ARS [6082-13630-005-00D] FX This article is part of USDA-ARS Project 6082-13630-005-00D "Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization." Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 33 TC 4 Z9 5 U1 7 U2 42 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD JUL 1 PY 2015 VL 157 BP 1 EP 7 DI 10.1016/j.jenvman.2015.04.010 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CJ7YF UT WOS:000355715600001 PM 25874587 ER PT J AU Centofanti, T Banuelos, G AF Centofanti, Tiziana Banuelos, Gary TI Evaluation of the halophyte Salsola soda as an alternative crop for saline soils high in selenium and boron SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Salinity; Halophyte; Salsola soda; Boron; Selenium; Alternative crops ID SAN-JOAQUIN VALLEY; DRAINAGE WATER; PLANTS; IRRIGATION; ACCUMULATION; PERFORMANCE; CALIFORNIA; TECHNOLOGY; SEDIMENTS AB Urbanization, industrial development, and intensive agriculture have caused soil contamination and land degradation in many areas of the world. Salinization is one important factor contributing to land degradation and it affects agricultural production and environmental quality. When salinization is combined with soil pollution by trace elements, as it occurs in many arid and semi-arid regions around the world, strategies to phyto-manage pollutants and sustain crop production need to be implemented. In this study, we present the case of saline soils in the West side of Central California which contain naturally-occurring selenium (Se), boron (B), and other salts, such as NaCl, CaCl2, Na2SO4, and Na2SeO4. To sustain crop production on Se- and B-laden arid saline soils, we investigated the potential of the halophyte "agretti" (Salsola soda L) as an alternative crop. The aim of our greenhouse study was to examine adaptability, B tolerance, and Se accumulation by S. soda grown on soils collected from a typical saline-laden field site located on the West side of the San Joaquin Valley (SJV). Our results showed that S. soda tolerates the saline (EC similar to 10 dS m(-1)) and B-laden soils (10 mg B L-1) of the SJV even with the additional irrigation of saline and B rich water (EC similar to 3 dS m(-1) and 4 mg B L-1). Under these growing conditions, the plant can accumulate high concentrations of Na (80 g Na kg(-1) DW), B (100 mg B kg(-1) DW), and Se (3-4 mg Se kg(-1) DW) without showing toxicity symptoms. Hence, S. soda showed promising potential as a plant species that can be grown in B-laden saline soils and accumulate and potentially manage excessive soluble Se and B in soil. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Centofanti, Tiziana] Calif State Univ Fresno, Ctr Irrigat Technol, Fresno, CA 93740 USA. [Banuelos, Gary] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Centofanti, T (reprint author), Calif State Univ Fresno, Ctr Irrigat Technol, 5370 N Chestnut M-S OF 18, Fresno, CA 93740 USA. EM tiziana.centofanti@gmail.com; gary.banuelos@ars.usda.gov FU American Chemical Society; SEED program scholarship FX We thank Christian Garcia for his helpful contribution to the completion of this study. Christian participated in this project as summer intern and recipient of the SEED program 2014 scholarship sponsored by the American Chemical Society. We also thank Justin King and Anna Allen for assisting with sample preparation and data processing. The authors thank Irvin Arroyo for his invaluable technical assistance and for analysis of mineral elements. NR 31 TC 2 Z9 2 U1 2 U2 31 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD JUL 1 PY 2015 VL 157 BP 96 EP 102 DI 10.1016/j.jenvman.2015.04.005 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CJ7YF UT WOS:000355715600009 PM 25897503 ER PT J AU Sanders, JL Zhou, Y Moulton, HM Moulton, ZX McLeod, R Dubey, JP Weiss, LM Kent, ML AF Sanders, J. L. Zhou, Y. Moulton, H. M. Moulton, Z. X. McLeod, R. Dubey, J. P. Weiss, L. M. Kent, M. L. TI The zebrafish, Danio rerio, as a model for Toxoplasma gondii: an initial description of infection in fish SO JOURNAL OF FISH DISEASES LA English DT Article DE Danio rerio; infection experiment ID SCOPHTHALMUS-MAXIMUS L; HUMAN-DISEASE; OOCYSTS; PATHOGENESIS; SURVIVAL; SYSTEM C1 [Sanders, J. L.; Kent, M. L.] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA. [Zhou, Y.; McLeod, R.] Univ Chicago, Dept Ophthalmol & Visual Sci, Chicago, IL 60637 USA. [Moulton, H. M.; Kent, M. L.] Oregon State Univ, Dept Biomed Sci, Corvallis, OR 97331 USA. [Moulton, Z. X.] Crescent Valley High Sch, Corvallis, OR USA. [Dubey, J. P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. [Weiss, L. M.] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10467 USA. [Weiss, L. M.] Montefiore Med Ctr, Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA. RP Sanders, JL (reprint author), Oregon State Univ, Dept Microbiol, 226 Nash Hall, Corvallis, OR 97331 USA. EM justin.sanders@oregonstate.edu FU Tartar Foundation; Department of Microbiology, Oregon State University; NIH [R01 U01AI082180-05]; Cornwell and Mann family; Morel family; Rooney-Alden family; Engel family; "Taking Out Toxo" FX The authors thank the Sinnhuber Aquatic Research Laboratory for providing zebrafish used in this study and the Oregon State University Veterinary Diagnostic Laboratory for histological slide preparation. This work was funded by the Tartar Foundation, Department of Microbiology, Oregon State University, NIH R01 U01AI082180-05 (RMc, YZ), and the Cornwell and Mann, Morel, Rooney-Alden, and Engel families and "Taking Out Toxo" (RMc, YZ). NR 32 TC 4 Z9 4 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD JUL PY 2015 VL 38 IS 7 BP 675 EP 679 DI 10.1111/jfd.12393 PG 5 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA CJ7LH UT WOS:000355676200009 PM 25951508 ER PT J AU Burri, BJ AF Burri, Betty J. TI Beta-cryptoxanthin as a source of vitamin A SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Review DE beta-cryptoxanthin; vitamin A; retinol; tangerines; metabolism; conversion ID SINGLE NUCLEOTIDE POLYMORPHISMS; GERBILS MERIONES-UNGUICULATUS; IN-VITRO DIGESTION; FLESHED SWEET-POTATO; ACUTE-PHASE RESPONSE; ZEA-MAYS SP.; MONGOLIAN GERBILS; CAROTENOID CONCENTRATIONS; BANGLADESHI WOMEN; DAILY CONSUMPTION AB Beta-cryptoxanthin is a common carotenoid that is found in fruit, and in human blood and tissues. Foods that are rich in beta-cryptoxanthin include tangerines, persimmons and oranges. Beta-cryptoxanthin has several functions that are important for human health, including roles in antioxidant defense and cell-to-cell communication. Most importantly, beta-cryptoxanthin is a precursor of vitamin A, which is an essential nutrient needed for eyesight, growth, development and immune response. We evaluate the evidence for beta-cryptoxanthin as a vitamin A-forming carotenoid in this paper. Observational, in vitro, animal model and human studies suggest that beta-cryptoxanthin has greater bioavailability from its common food sources than do alpha- and beta-carotene from theirs. Although beta-cryptoxanthin appears to be a poorer substrate for beta-carotene 15,15 oxygenase than is beta-carotene, animal model and human studies suggest that the comparatively high bioavailability of beta-cryptoxanthin from foods makes beta-cryptoxanthin-rich foods equivalent to beta-carotene-rich foods as sources of vitamin A. These results mean that beta-cryptoxanthin-rich foods are probably better sources of vitamin A, and more important for human health in general, than previously assumed. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Burri, BJ (reprint author), USDA ARS, Western Human Nutr Res Ctr, 430W Hlth Sci Dr, Davis, CA 95616 USA. EM Betty.burri@ars.usda.gov NR 83 TC 4 Z9 5 U1 8 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD JUL PY 2015 VL 95 IS 9 BP 1786 EP 1794 DI 10.1002/jsfa.6942 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CJ6UQ UT WOS:000355630400003 PM 25270992 ER PT J AU LeBlanc, N Kinkel, LL Kistler, HC AF LeBlanc, Nicholas Kinkel, Linda L. Kistler, H. Corby TI Soil Fungal Communities Respond to Grassland Plant Community Richness and Soil Edaphics SO MICROBIAL ECOLOGY LA English DT Article DE Fungi; Fusarium; Metagenomics; Rhizosphere ID PHYLOGENETIC DIVERSITY; SPECIES COMPLEX; RAIN-FORESTS; FUSARIA; LIFE; TREE; EVOLUTION; GRADIENT; ORIGIN AB Fungal communities in soil have significant influences on terrestrial ecosystem dynamics, yet our understanding of the drivers of fungal diversity and community structure in soil is limited. Fungal communities associated with the rhizosphere of four native perennial grassland plant species, two legumes and two grasses, grown in monoculture and polyculture in a long-term field experiment were characterized. Reference databases were developed for, and amplicon libraries sequenced from, multiple-copy rRNA and single-copy protein-coding loci. Clustering and alignment-based pipelines were utilized to evaluate differences in fungal community structure and diversity in response to plant host, plant community richness, and soil edaphics. Fungal diversity increased in the rhizosphere of plants growing in polyculture plant communities as compared to monoculture plant communities. Fungal community structure was differentiated between legumes and grasses growing in monoculture but not in polyculture. To specifically monitor fungi in the genus Fusarium in the soil, the protein-coding locus was used to increase phylogenetic resolution and enrich for this taxon. These data show that fungal community richness and structure are strongly linked with plant community dynamics and associated soil edaphic characteristics in these grassland soils. C1 [LeBlanc, Nicholas; Kinkel, Linda L.; Kistler, H. Corby] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Kistler, H. Corby] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kistler, HC (reprint author), Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM hckist@umn.edu FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011-67019-30200] FX We thank Joey Spatafora, Frank Kauff, Francois Lutzoni, and David Geiser for supplying a subset of the sequences used as references in this study, Georgiana May for supplying 454 compatible LSU primers, Chris Wright at the Roy J. Carver Sequencing Center, Dave Tilman at the Cedar Creek Ecosystem Science Reserve LTER, as well as Matt Bakker, Dan Schlatter, and Lindsey Otto-Hanson for their contributions to soil sampling and processing. We also acknowledge the comments from anonymous reviewers which helped improve the quality and clarity of this manuscript. This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67019-30200 from the USDA National Institute of Food and Agriculture. NR 48 TC 7 Z9 7 U1 8 U2 58 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0095-3628 EI 1432-184X J9 MICROB ECOL JI Microb. Ecol. PD JUL PY 2015 VL 70 IS 1 BP 188 EP 195 DI 10.1007/s00248-014-0531-1 PG 8 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA CJ7RC UT WOS:000355694900018 PM 25399511 ER PT J AU Nolan, BT Malone, RW Doherty, JE Barbash, JE Ma, LW Shaner, DL AF Nolan, Bernard T. Malone, Robert W. Doherty, John E. Barbash, Jack E. Ma, Liwang Shaner, Dale L. TI Data worth and prediction uncertainty for pesticide transport and fate models in Nebraska and Maryland, United States SO PEST MANAGEMENT SCIENCE LA English DT Article DE degradates; metolachlor; parameter estimation; prediction uncertainty; RZWQM ID 5 AGRICULTURAL SETTINGS; WATER-QUALITY; UNSATURATED ZONES; DEGRADATION; RZWQM; CALIBRATION; HERBICIDES; FLOW; USA; VALIDATION AB BACKGROUNDComplex environmental models are frequently extrapolated to overcome data limitations in space and time, but quantifying data worth to such models is rarely attempted. The authors determined which field observations most informed the parameters of agricultural system models applied to field sites in Nebraska (NE) and Maryland (MD), and identified parameters and observations that most influenced prediction uncertainty. RESULTSThe standard error of regression of the calibrated models was about the same at both NE (0.59) and MD (0.58), and overall reductions in prediction uncertainties of metolachlor and metolachlor ethane sulfonic acid concentrations were 98.0 and 98.6% respectively. Observation data groups reduced the prediction uncertainty by 55-90% at NE and by 28-96% at MD. Soil hydraulic parameters were well informed by the observed data at both sites, but pesticide and macropore properties had comparatively larger contributions after model calibration. CONCLUSIONSAlthough the observed data were sparse, they substantially reduced prediction uncertainty in unsampled regions of pesticide breakthrough curves. Nitrate evidently functioned as a surrogate for soil hydraulic data in well-drained loam soils conducive to conservative transport of nitrogen. Pesticide properties and macropore parameters could most benefit from improved characterization further to reduce model misfit and prediction uncertainty. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Nolan, Bernard T.] US Geol Survey, Reston, VA 20192 USA. [Malone, Robert W.] USDA, Ames, IA USA. [Doherty, John E.] Watermark Numer Comp, Corinda, Australia. [Doherty, John E.] Flinders Univ S Australia, Natl Ctr Groundwater Res & Training, Bedford Pk, SA, Australia. [Barbash, Jack E.] US Geol Survey, Tacoma, WA USA. [Ma, Liwang; Shaner, Dale L.] USDA, Ft Collins, CO USA. RP Nolan, BT (reprint author), US Geol Survey, Natl Ctr 413, Reston, VA 20192 USA. EM btnolan@usgs.gov NR 38 TC 1 Z9 1 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD JUL PY 2015 VL 71 IS 7 BP 972 EP 985 DI 10.1002/ps.3875 PG 14 WC Agronomy; Entomology SC Agriculture; Entomology GA CJ6UT UT WOS:000355630800013 PM 25132142 ER PT J AU Alarcon-Reverte, R Garcia, A Watson, SB Abdallah, I Sabate, S Hernandez, MJ Dayan, FE Fischer, AJ AF Alarcon-Reverte, Rocio Garcia, Alejandro Watson, Susan B. Abdallah, Ibrahim Sabate, Sebastian Hernandez, Maria J. Dayan, Franck E. Fischer, Albert J. TI Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California SO PEST MANAGEMENT SCIENCE LA English DT Article DE 5-enolpyruvylshikimate-3-phosphate synthase; EPSPS expression; EPSPS inhibition assay; polyploidy; resistance mechanism ID HORSEWEED CONYZA-CANADENSIS; RYEGRASS LOLIUM-RIGIDUM; L. CRONQ. BIOTYPES; 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; GENE AMPLIFICATION; HERBICIDE RESISTANCE; SHIKIMATE PATHWAY; AMARANTHUS-TUBERCULATUS; REDUCED TRANSLOCATION; ACETOLACTATE SYNTHASE AB BACKGROUNDEchinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide, was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of California, were used to generate three GR and one S selfed lines to study possible mechanisms involved in glyphosate resistance. RESULTSBased on the amount of glyphosate required to kill 50% of the plants (LD50)(,) GR lines were 4-9-fold more resistant than S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption, translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants. Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants. CONCLUSIONIt is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same population, individuals from different populations may carry different resistance mechanisms and different mechanisms can act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant expression of EPSPS sensitivity to glyphosate. (c) 2014 Society of Chemical Industry C1 [Alarcon-Reverte, Rocio; Garcia, Alejandro; Fischer, Albert J.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Watson, Susan B.; Dayan, Franck E.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS USA. [Abdallah, Ibrahim] Cairo Univ, Dept Pesticides, Fac Agr, Giza, Egypt. [Sabate, Sebastian] Estn Expt Agroind Obispo Colombres, San Miguel De Tucuman, Tucuman, Argentina. [Hernandez, Maria J.] Pontificia Univ Catolica Chile, Fac Agron & Ingn Forestal, Dept Ciencias Vegetales, Santiago, Chile. RP Fischer, AJ (reprint author), Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. EM ajfischer@ucdavis.edu RI Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 FU California Rice Research Board; Instituto Nacional de Investigacion Agropecuaria of Uruguay FX The authors are grateful to the California Rice Research Board and to the Instituto Nacional de Investigacion Agropecuaria of Uruguay for financial support. They would like to thank Dr Stephen Pearce for his assistance with gene sequencing and expression experiments. They would also like to express their gratitude for the collaboration of Dr Douglas Cook, Department of Plant Pathology, and Drs Jorge Dubcovsky and Marie Jasieniuk, Department of Plant Sciences at University of California, Davis, who facilitated equipment for molecular assays. Thanks also to Drs Daniela Ribeiro and Vijay Nandula for their useful comments concerning this manuscript. NR 88 TC 7 Z9 8 U1 2 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD JUL PY 2015 VL 71 IS 7 BP 996 EP 1007 DI 10.1002/ps.3878 PG 12 WC Agronomy; Entomology SC Agriculture; Entomology GA CJ6UT UT WOS:000355630800015 PM 25115401 ER PT J AU Szymanski, RM Morris, CF AF Szymanski, Ryan M. Morris, Craig F. TI Internal structure of carbonized wheat (Triticum spp.) grains: relationships to kernel texture and ploidy SO VEGETATION HISTORY AND ARCHAEOBOTANY LA English DT Article DE Charring; Carbonization; Archaeobotany; Ploidy; Glume wheats ID NEOLITHIC CATALHOYUK; MOLECULAR-GENETICS; POLYPLOID WHEAT; NORTHERN SYRIA; EMMER WHEAT; DOMESTICATION; HARDNESS; GENES; PUROINDOLINES; HISTORY AB The identification of wheat grains to the species level is problematic in many archaeobotanical samples, yet this is key to better understanding wheat phylogeny and agricultural trajectories. This study was conducted to see if the pronounced differences in kernel texture (grain hardness) which exist among einkorn (very soft), emmer/durum (very hard), and bread wheat/spelt (soft) would manifest themselves after charring (carbonization). Grains of these three species were either broken and then charred, or charred and then broken (charring at 250, 270 and 400 A degrees C). All specimens were then examined using field emission scanning electron microscopy. At 250 A degrees C, grains broken before charring showed distension across the broken exposed endosperm surface. In the two soft wheats (Triticum monococcum, T. spelta; einkorn and spelt), starch granules were still evident, whereas in T. dicoccum, emmer, the fracture surface was nearly smooth. When charring occurred before breaking, a flat fracture face resulted. At 270 A degrees C, distension increased for grains broken before charring whereas in those broken after charring, the fracture plane was again perpendicular to the longitudinal axis and was present as an open sponge-like topography. At 400 A degrees C, distension was pronounced and represented "puffing" in grains that were broken before charring. Those that were charred and then broken produced a markedly open porous structure, but again with aspects of an angular fracture plane. In conclusion, several morphological features related to grain hardness, and therefore ploidy and genus were present at the lower charring temperatures. These differences largely disappeared at 400 A degrees C. Characteristics of the carbonized grains provide some diagnostic features as to possible food processing or damage during recovery. C1 [Szymanski, Ryan M.] Washington State Univ, Dept Anthropol, Pullman, WA 99164 USA. [Morris, Craig F.] Washington State Univ, USDA, ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA. RP Morris, CF (reprint author), Washington State Univ, USDA, ARS, Western Wheat Qual Lab, E-202 Food Sci & Human Nutr Facil East,646394, Pullman, WA 99164 USA. EM morrisc@wsu.edu NR 55 TC 0 Z9 0 U1 8 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0939-6314 EI 1617-6278 J9 VEG HIST ARCHAEOBOT JI Veg. Hist. Archaeobot. PD JUL PY 2015 VL 24 IS 4 BP 503 EP 515 DI 10.1007/s00334-014-0508-5 PG 13 WC Plant Sciences; Paleontology SC Plant Sciences; Paleontology GA CJ8PO UT WOS:000355765900004 ER PT J AU Trivella, A Stawinoga, M Dayan, FE Cantrell, CL Mazellier, P Richard, C AF Trivella, Aurelien Stawinoga, Malgorzata Dayan, Franck E. Cantrell, Charles L. Mazellier, Patrick Richard, Claire TI Photolysis of natural beta-triketonic herbicides in water SO WATER RESEARCH LA English DT Article DE Photolysis; Natural herbicides; Water; Photoproducts; Dissipation ID P-HYDROXYPHENYLPYRUVATE DIOXYGENASE; LEPTOSPERMUM-SCOPARIUM; SOLAR LIGHT; TAUTOMERISM; IRRADIATION; MANUKA; OILS; WAX AB The fate of four natural beta-triketones (leptospermone, isoleptospermone, grandiflorone and flavesone, pK(a) = 4.0-4.5) in aqueous solution, in the dark and upon simulated solar light irradiation was investigated. In anionic form, beta-triketones undergo slow dark oxidation and photolysis with polychromatic quantum yields varying from 1.2 x 10(-4) to 3.7 x 10(-4). Leptospermone and grandiflorone are the most photolabile compounds. In,molecular form, beta-triketones are rather volatile. Polychromatic quantum yields between 1.2 x 10(-3) and 1.8 x 10(-3) could be measured for leptospermone and grandiflorone. They are 3-5 times higher than for the anionic forms. Photooxidation on the carbon atom bearing the acidic hydrogen atom is the main oxidation reaction, common to all the beta-triketones whatever their ionization state. However, leptospermone shows a special photoreactivity. In molecular form, it mainly undergoes photoisomerization. Based on this work, the half-lives of beta-triketones in surface waters should be comprised between 7 and 23 days. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Trivella, Aurelien; Stawinoga, Malgorzata; Richard, Claire] Univ Clermont Ferrand, Clermont Univ, Inst Chim Clermont Ferrand, F-63000 Clermont Ferrand, France. [Trivella, Aurelien; Stawinoga, Malgorzata; Richard, Claire] CNRS, UMR 6296, ICCF, F-63171 Aubiere, France. [Trivella, Aurelien; Mazellier, Patrick] Univ Bordeaux, EPOC Lab Phys & Tox Chim Environm, F-33405 Talence, France. [Trivella, Aurelien; Mazellier, Patrick] CNRS, UMR 5805, EPOC LPTC, F-33405 Talence, France. [Dayan, Franck E.; Cantrell, Charles L.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. RP Richard, C (reprint author), CNRS, UMR 6296, ICCF, F-63171 Aubiere, France. EM claire.richard@univ-bpclermont.fr RI Dayan, Franck/A-7592-2009; richard, claire/E-9405-2011; OI Dayan, Franck/0000-0001-6964-2499; Trivella, Aurelien/0000-0002-6807-0609 NR 24 TC 7 Z9 7 U1 2 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD JUL 1 PY 2015 VL 78 BP 28 EP 36 DI 10.1016/j.watres.2015.03.026 PG 9 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA CJ8SS UT WOS:000355774400004 PM 25898250 ER PT J AU Rigby, JR Yin, J Albertson, JD Porporato, A AF Rigby, J. R. Yin, Jun Albertson, John D. Porporato, Amilcare TI Approximate Analytical Solution to Diurnal Atmospheric Boundary-Layer Growth Under Well-Watered Conditions SO BOUNDARY-LAYER METEOROLOGY LA English DT Article DE Analytical solution; Atmospheric boundary layer; Bowen ratio; Equilibrium evaporation; Evaporative fraction; Mixed-layer model; Perturbation theory ID DAYTIME MIXED-LAYER; EQUILIBRIUM EVAPORATION; APPLIED-MODEL; LAND-SURFACE; INVERSION; ENTRAINMENT; CONVECTION; FEEDBACKS; HEIGHT; BUDGETS AB Simplified numerical models of the atmospheric boundary layer (ABL) are useful both for understanding the underlying dynamics and potentially providing parsimonious modelling approaches for inclusion in larger models. Herein the governing equations of a simplified slab model of the uniformly mixed, purely convective, diurnal ABL are shown to allow immediate solutions for the potential temperature and specific humidity as functions of the ABL height and net radiation when expressed in integral form. By employing a linearized saturation vapour relation, the height of the mixed layer is shown to obey a non-linear ordinary differential equation with quadratic dependence on ABL height. A perturbation solution provides general analytical approximations, of which the leading term is shown to represent the contribution under equilibrium evaporation. These solutions allow the diurnal evolution of the height, potential temperature, and specific humidity (i.e., also vapour pressure deficit) of the mixed layer to be expressed analytically for arbitrary radiative forcing functions. C1 [Rigby, J. R.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Yin, Jun; Albertson, John D.; Porporato, Amilcare] Duke Univ, Dept Civil & Environm Engn, Durham, NC 27708 USA. RP Rigby, JR (reprint author), USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. EM jr.rigby@ars.usda.gov; jun.yin@duke.edu; john.albertson@duke.edu; amilcare@duke.edu OI Rigby, James/0000-0002-5611-6307 FU National Aeronautics and Space Administration (NASA) [NNX09AN76G]; National Science Foundation [NSF-CBET-1033467, NSF-EAR-0838301, NSF-EAR-1331846, NSF-EAR-1316258, NSF FESD 1338694]; US DOE through the Office of Biological and Environmental Research, Terrestrial Carbon Processes program [DE-SC0006967]; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [201167003-30222]; U.S. Department of Energy FX We gratefully acknowledge funding from National Aeronautics and Space Administration (NASA grant NNX09AN76G), National Science Foundation (NSF-CBET-1033467, NSF-EAR-0838301, NSF-EAR-1331846, and NSF-EAR-1316258, NSF FESD 1338694), the US DOE through the Office of Biological and Environmental Research, Terrestrial Carbon Processes program (DE-SC0006967), as well as the Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture (201167003-30222). Data were obtained from the Atmospheric Radiation Measurement Program sponsored by the U.S. Department of Energy. The comments and useful criticisms of anonymous reviewers are gratefully acknowledged. NR 41 TC 2 Z9 2 U1 0 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0006-8314 EI 1573-1472 J9 BOUND-LAY METEOROL JI Bound.-Layer Meteor. PD JUL PY 2015 VL 156 IS 1 BP 73 EP 89 DI 10.1007/s10546-015-0018-8 PG 17 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CJ0GG UT WOS:000355153200005 ER PT J AU Hartman, GL Chang, HX Leandro, LF AF Hartman, G. L. Chang, H. -X. Leandro, L. F. TI Research advances and management of soybean sudden death syndrome SO CROP PROTECTION LA English DT Article DE Sudden death syndrome; Fusarium virguliforme; Integrated management ID F-SP GLYCINES; FUSARIUM-SOLANI; FIELD-RESISTANCE; CYST-NEMATODE; HETERODERA-GLYCINES; ROOT COLONIZATION; SYNDROME PATHOGEN; UNITED-STATES; CAUSAL AGENT; SP. GLYCINES AB Fusarium virguliforme causes soybean sudden death syndrome (SDS) in the United States. The disease was first observed in Arkansas in 1971, and since has been reported in most soybean-producing states, with a general movement from the southern to the northern states. In addition to F. virguliforme, three other species, Fusarium brasiliense, Fusarium crassistipitatum, and Fusarium tucumaniae, have been reported to cause SDS in South America. Yield losses caused by F. virguliforme range from slight to 100%. Severely infected plants often have increased flower and pod abortion, reduced seed size, increased defoliation, and prematurely senescence. Foliar symptoms observed in the field are most noticeable from mid to late reproductive growth stages. To manage SDS, research on crop rotations, soil types, tillage practices, seed treatments, and the development and utilization of host resistance has been investigated. This review focuses on what is known about E virguliforme, the management of SDS in the United States, and how genetic engineering along with other traditional management options may be needed as integrated approaches to manage SDS. Published by Elsevier Ltd. C1 [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Chang, H. -X.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Leandro, L. F.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50010 USA. RP Hartman, GL (reprint author), Univ Illinois, USDA ARS, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Chang, Hao-Xun/0000-0002-4667-7741 NR 77 TC 14 Z9 14 U1 9 U2 52 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUL PY 2015 VL 73 SI SI BP 60 EP 66 DI 10.1016/j.cropro.2015.01.017 PG 7 WC Agronomy SC Agriculture GA CJ3EL UT WOS:000355366100009 ER PT J AU Shapiro, C Arthaud, G Casey, F Hogan, D AF Shapiro, Carl Arthaud, Greg Casey, Frank Hogan, Dianna TI Ecosystem services science, practice, and policy: Perspectives from ACES, A Community on Ecosystem Services SO ECOLOGICAL ECONOMICS LA English DT Editorial Material C1 [Shapiro, Carl; Casey, Frank] US Geol Survey, Sci & Decis Ctr, Reston, VA 22092 USA. [Arthaud, Greg] US Forest Serv, Res & Dev, Washington, DC 20250 USA. [Hogan, Dianna] US Geol Survey, Eastern Geog Sci Ctr, Reston, VA 22092 USA. RP Shapiro, C (reprint author), US Geol Survey, Sci & Decis Ctr, 959 Natl Ctr, Reston, VA 22092 USA. NR 3 TC 1 Z9 1 U1 3 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 EI 1873-6106 J9 ECOL ECON JI Ecol. Econ. PD JUL PY 2015 VL 115 SI SI BP 1 EP 2 DI 10.1016/j.ecolecon.2015.04.001 PG 2 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA CI9AC UT WOS:000355061800001 ER PT J AU Horvath, DP Hansen, SA Moriles-Miller, JP Pierik, R Yan, CH Clay, DE Scheffler, B Clay, SA AF Horvath, David P. Hansen, Stephanie A. Moriles-Miller, Janet P. Pierik, Ronald Yan, Changhui Clay, David E. Scheffler, Brian Clay, Sharon A. TI RNAseq reveals weed-induced PIF3-like as a candidate target to manipulate weed stress response in soybean SO NEW PHYTOLOGIST LA English DT Article DE Glycine max (soybean); PHYTOCHROME INTERACTING FACTOR 3 (PIF3); shade avoidance response; transcriptomics; weed stress ID SHADE-AVOIDANCE RESPONSES; SHOCK TRANSCRIPTION FACTOR; MAX L MERR; ARABIDOPSIS-THALIANA; LIGHT QUALITY; INTRASPECIFIC COMPETITION; PHYTOCHROME GENE; PLANT-RESPONSES; CRITICAL PERIOD; CORN RESPONSE AB Weeds reduce yield in soybeans (Glycine max) through incompletely defined mechanisms. The effects of weeds on the soybean transcriptome were evaluated in field conditions during four separate growing seasons. RNASeq data were collected from six biological samples of soybeans growing with or without weeds. Weed species and the methods to maintain weed-free controls varied between years to mitigate treatment effects, and to allow detection of general soybean weed responses. Soybean plants were not visibly nutrient- or water-stressed. We identified 55 consistently downregulated genes in weedy plots. Many of the downregulated genes were heat shock genes. Fourteen genes were consistently upregulated. Several transcription factors including a PHYTOCHROME INTERACTING FACTOR 3-like gene (PIF3) were included among the upregulated genes. Gene set enrichment analysis indicated roles for increased oxidative stress and jasmonic acid signaling responses during weed stress. The relationship of this weed-induced PIF3 gene to genes involved in shade avoidance responses in Arabidopsis provide evidence that this gene may be important in the response of soybean to weeds. These results suggest that the weed-induced PIF3 gene will be a target for manipulating weed tolerance in soybean. C1 [Horvath, David P.] ARS, Sunflower & Plant Biol Res Unit, USDA, Fargo, ND 58102 USA. [Hansen, Stephanie A.; Moriles-Miller, Janet P.; Clay, David E.; Clay, Sharon A.] S Dakota State Univ, Dept Plant Biol, Brookings, SD 57006 USA. [Pierik, Ronald] Univ Utrecht, Grad Sch Life Sci, Utrecht, Netherlands. [Yan, Changhui] N Dakota State Univ, Dept Comp Sci, Fargo, ND 58105 USA. [Scheffler, Brian] ARS, MSA Genom Lab, USDA, Stoneville, MS 38776 USA. RP Horvath, DP (reprint author), ARS, Sunflower & Plant Biol Res Unit, USDA, Fargo, ND 58102 USA. EM David.Horvath@ars.usda.gov RI Pierik, Ronald/B-9591-2011; OI Pierik, Ronald/0000-0002-5320-6817; Scheffler, Brian/0000-0003-1968-8952 FU South Dakota Soybean Research and Promotion Council FX Funding in part was provided by the South Dakota Soybean Research and Promotion Council. Special thanks to Dr Greta Gramig and Dr Clarence Swanton and members of his lab past and present for a critical review of this manuscript. NR 80 TC 3 Z9 4 U1 11 U2 65 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JUL PY 2015 VL 207 IS 1 BP 196 EP 210 DI 10.1111/nph.13351 PG 15 WC Plant Sciences SC Plant Sciences GA CJ1ZX UT WOS:000355284900019 PM 25711503 ER PT J AU Zhang, QH Yang, L Zhang, J Wu, MD Chen, WD Jiang, DH Li, GQ AF Zhang, Qinghua Yang, Long Zhang, Jing Wu, Mingde Chen, Weidong Jiang, Daohong Li, Guoqing TI Production of anti-fungal volatiles by non-pathogenic Fusarium oxysporum and its efficacy in suppression of Verticillium wilt of cotton SO PLANT AND SOIL LA English DT Article DE Fusarium oxysporum CanR-46; Volatile organic compounds; Verticillium dahliae; Biological control; Fumigation ID FUNGUS MUSCODOR-ALBUS; PLANT-GROWTH; SEEDLING DISEASES; SUGAR-BEET; BIOCONTROL; STRAIN; ARABIDOPSIS; RESISTANCE; SUBSTANCES; VIRULENCE AB The study aimed at identifying volatile organic compounds (VOCs) produced by the non-pathogenic Fusarium oxysporum (Fo) strain CanR-46, and to determine the anti-fungal spectrum and the control efficacy of the Fo-VOCs. The Fo-VOCs were identified by GC-MS. The antifungal activity of the Fo-VOCs was tested using the double-dish method. Soil contaminated with Verticillium dahliae was fumigated with the Fo-VOCs to test the control efficacy. The GFP-tagged derivative strain CanR-46GFP was tested to colonize cotton roots for prevention of infection by V. dahliae. Nineteen VOCs were identified with eremophila-1 (10),11-diene being the most abundant. The Fo-VOCs inhibited mycelial growth of 14 fungal species including V. dahliae, delayed V. dahliae conidial germination, suppressed V. dahliae germ-tube elongation, and caused V. dahliae hyphal shriveling and collapse. Four synthetic chemicals, 5-hexenoic acid, limonene, octanoic acid and 3,4-2H-dihydropyran, in the Fo-VOCs profile showed antifungal activity against V. dahliae. The Fo-VOCs significantly (P < 0.05) reduced severity of Verticillium wilt of cotton compared to the control. CanR-46GFP extensively colonized the cotton root tissues, thus effectively prevented V. dahliae infection. The VOC-producing fungus F. oxysporum CanR-46 is a promising biocontrol agent against V. dahliae. C1 [Zhang, Qinghua; Yang, Long; Zhang, Jing; Wu, Mingde; Jiang, Daohong; Li, Guoqing] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. [Zhang, Qinghua; Yang, Long; Zhang, Jing; Wu, Mingde; Jiang, Daohong; Li, Guoqing] Huazhong Agr Univ, Key Lab Plant, Pathol Hubei Prov, Wuhan 430070, Peoples R China. [Zhang, Qinghua] Jiangxi Acad Agr Sci, Inst Vegetables & Flowers, Nanchang 330200, Jiangxi, Peoples R China. [Chen, Weidong] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. RP Li, GQ (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. EM zhangqinghua23@gmail.com; yanglong@mail.hzau.edu.cn; zhangjing1007@mail.hzau.edu.cn; mingde@mail.hzau.edu.cn; w-chen@wsu.edu; daohongjiang@mail.hzau.edu.cn; guoqingli@mail.hzau.edu.cn FU Natural Science Foundation of China [31070122]; R and D Special Fund for Public Welfare Industry (Agriculture) of China [201103016, 201303025] FX This research was funded by the Natural Science Foundation of China (Grant No. 31070122) and the R and D Special Fund for Public Welfare Industry (Agriculture) of China (Grant No. 201103016 and 201303025). Conflict of Interest: All the authors declare that they have no conflict of interest. NR 38 TC 3 Z9 3 U1 8 U2 59 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD JUL PY 2015 VL 392 IS 1-2 BP 101 EP 114 DI 10.1007/s11104-015-2448-y PG 14 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA CJ0FX UT WOS:000355152300009 ER PT J AU Bakker, MG Chaparro, JM Manter, DK Vivanco, JM AF Bakker, Matthew G. Chaparro, Jacqueline M. Manter, Daniel K. Vivanco, Jorge M. TI Impacts of bulk soil microbial community structure on rhizosphere microbiomes of Zea mays SO PLANT AND SOIL LA English DT Article DE Soil microbiome; Rhizosphere; Community dynamics; Zea mays; Illumina MiSeq ID BACTERIAL COMMUNITIES; GENETIC-STRUCTURE; ROOT MICROBIOME; ARABLE SOILS; MAIZE; PLANT; POPULATIONS; DIVERSITY; GROWTH; MICROORGANISMS AB It has frequently been shown that plants interact with soils to shape rhizosphere microbiomes. However, previous work has not distinguished between effects of soil properties per se, and effects attributable to the resident microbial communities of those soils. We aimed to test whether differences in the structure of bulk soil microbial communities, within a given soil type, would carry over to impact the structure of the rhizosphere microbial community. We used repeated chemical amendments to develop divergent bulk soil microbial community starting points from which rhizosphere development proceeded. Additionally, we contrasted rhizosphere microbiomes associated with two different cultivars of corn (Zea mays). A wide range of bacterial and archaeal taxa responded to chemical resource amendments, which reduced bulk soil microbiome diversities. Corn genotypes P9714XR and 35F40 had largely similar impacts on rhizosphere microbiome development, although significant differences were evident in select treatments. Notably, in cases where resource amendments altered bulk soil microbial community composition, legacy effects persisted into the rhizosphere. Our results suggest that rhizosphere microbial communities may develop into different states depending on site history and prior selective events. This work advances our understanding of soil microbiome dynamics and responsiveness to change in the form of simple resource amendments and the development of the rhizosphere. C1 [Bakker, Matthew G.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Chaparro, Jacqueline M.; Vivanco, Jorge M.] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. [Manter, Daniel K.] ARS, USDA, Soil Plant Nutrient Res Grp, Ft Collins, CO 80526 USA. RP Bakker, MG (reprint author), ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM Matt.Bakker@ars.usda.gov FU USDA NIFA [2011-67012-30938] FX Shawn Salley provided assistance in locating soils and performed the texture analysis. MGB was supported by a USDA NIFA Postdoctoral Fellowship grant (2011-67012-30938) for a portion of this work. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This article was the work of U.S. Government employees engaged in their official duties and is exempt from copyright. NR 48 TC 4 Z9 4 U1 11 U2 131 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD JUL PY 2015 VL 392 IS 1-2 BP 115 EP 126 DI 10.1007/s11104-015-2446-0 PG 12 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA CJ0FX UT WOS:000355152300010 ER PT J AU Cheung, AK AF Cheung, Andrew K. TI Specific functions of the Rep and Rep' proteins of porcine circovirus during copy-release and rolling-circle DNA replication SO VIROLOGY LA English DT Article DE Porcine circovirus; Rolling-circle DNA replication; Minus-genome primer ID TYPE-1; TRANSCRIPTION; TERMINATION; INITIATION; PLASMID; ORIGIN AB The roles of two porcine circovirus replication initiator proteins, Rep and Rep', in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates the single-stranded circular (ssc) genome from the displaced DNA strand. In the process, a minus-genome primer (MGP) necessary for complementary-strand synthesis, from ssc to ccc, is synthesized. Rep' cleaves the growing nascent-strand to regenerate the parent ccc molecule. In the process, a Rep'-DNA hybrid containing the right palindromic sequence (at the origin of DNA replication) is generated. Analysis of the virus particle showed that it is composed of four components: ssc, MGP, capsid protein and a novel Rep-related protein (designated Protein-3). (C) 2015 Published by Elsevier Inc. C1 USDA ARS, Natl Anim Dis Ctr, Virus & Prion Dis Res Unit, Ames, IA 50010 USA. RP Cheung, AK (reprint author), USDA ARS, Natl Anim Dis Ctr, Virus & Prion Dis Res Unit, POB 70, Ames, IA 50010 USA. EM mwsympo@hotmail.com FU USDA, Agricultural Research Service [5030-32000-108-00D] FX The author thanks Drs. M. Kehrli, J. Ridpath and K. Lager for critical reading of the manuscript, and Mr. N. Otis for excellent technical support. This work was supported by the USDA, Agricultural Research Service Project #5030-32000-108-00D. NR 15 TC 0 Z9 0 U1 1 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JUL PY 2015 VL 481 BP 43 EP 50 DI 10.1016/j.virol.2015.01.004 PG 8 WC Virology SC Virology GA CI9CW UT WOS:000355069000005 PM 25768890 ER PT J AU Kappes, MA Miller, CL Faaberg, KS AF Kappes, Matthew A. Miller, Cathy L. Faaberg, Kay S. TI Porcine reproductive and respiratory syndrome virus nonstructural protein 2 (nsp2) topology and selective isoform integration in artificial membranes SO VIROLOGY LA English DT Article DE Porcine reproductive and respiratory syndrome virus; Arterivirus; Nonstructural protein 2; Membrane topology; Structural protein; Protein isomers ID ARTERIVIRUS REPLICATION COMPLEX; SIGNAL RECOGNITION PARTICLE; TAIL-ANCHORED PROTEINS; ENDOPLASMIC-RETICULUM; TRANSMEMBRANE TOPOLOGY; TOPOGENIC SIGNALS; RNA REPLICATION; P-GLYCOPROTEIN; PREDICTION; DEGRADATION AB The membrane insertion and topology of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus (PRRSV) strain VR-2332 was assessed using a cell free translation system in the presence or absence of artificial membranes. Expression of PRRSV nsp2 in the absence of all other viral factors resulted in the genesis of both full-length nsp2 as well as a select number of C-terminal nsp2 isoforms. Addition of membranes to the translation stabilized the translation reaction, resulting in predominantly full-length nsp2 as assessed by immunoprecipitation. Analysis further showed full-length nsp2 strongly associates with membranes, along with two additional large nsp2 isoforms. Membrane integration of full-length nsp2 was confirmed through high-speed density fractionation, protection from protease digestion, and immunoprecipitation. The results demonstrated that nsp2 integrated into the membranes with an unexpected topology, where the amino (N)-terminal (cytoplasmic) and C-terminal (luminal) domains were orientated on opposite sides of the membrane surface. Published by Elsevier Inc. C1 [Kappes, Matthew A.; Faaberg, Kay S.] USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Kappes, Matthew A.; Miller, Cathy L.] Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA USA. RP Faaberg, KS (reprint author), USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, Mailstop 2S-209,1920 Dayton Ave, Ames, IA 50010 USA. EM kay.faaberg@ars.usda.gov OI Miller, Cathy/0000-0002-2601-4422 FU National Pork Board [13-196]; Boehringer Ingelheim Vetmedica, Inc. [991-3625-791]; USDA [3625-32000-108-00D] FX Funding, wholly or in part, was provided by The National Pork Board (#13-196), Boehringer Ingelheim Vetmedica, Inc. (Grant number 991-3625-791), and Project 3625-32000-108-00D of the USDA. USDA is an equal opportunity provider and employer. NR 69 TC 3 Z9 4 U1 1 U2 7 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JUL PY 2015 VL 481 BP 51 EP 62 DI 10.1016/j.virol.2015.01.028 PG 12 WC Virology SC Virology GA CI9CW UT WOS:000355069000006 PM 25768891 ER PT J AU Duveneck, MJ Thompson, JR Wilson, BT AF Duveneck, Matthew J. Thompson, Jonathan R. Wilson, B. Tyler TI An imputed forest composition map for New England screened by species range boundaries SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Nearest-neighbor imputation; New England; Forest inventory; Tree species distribution ID NEAREST-NEIGHBOR IMPUTATION; VEGETATION DYNAMICS; CLIMATE-CHANGE; LAND-USE; MODEL; SIMULATION; USA; BIOMASS; CLASSIFICATION; PREDICTIONS AB Initializing forest landscape models (FLMs) to simulate changes in tree species composition requires accurate fine-scale forest attribute information mapped continuously over large areas. Nearest-neighbor imputation maps, maps developed from multivariate imputation of field plots, have high potential for use as the initial condition within FLMs, but the tendency for field plots to be imputed over large geographical distances can result in species being mapped outside of their home ranges, which is problematic. We developed an approach for evaluating and imputing field plots based on their similarity across multiple spatial environmental variates, their species composition, and their geographical distance between source and imputation to produce a map that is appropriate for initializing an FLM. We used this approach to map 13 million ha of forest throughout the six New England states (Rhode Island, Connecticut, Massachusetts, New Hampshire, Vermont, and Maine). Using both independent state forest and, more extensive, ecoregion validation data sets, we compared the imputation map to field inventory data, based on the dissimilarity of tree community composition and the rank order correlation of tree species abundance. Average Bray-Curtis dissimilarity between the imputation map and field plots was 0.32 and 0.12, for the state forest and ecoregion validation data sets, respectfully. Average Spearman rank order correlation was 0.81 and 0.93 for the state forest and ecoregion validation data sets, respectfully. Our analyses suggest that this approach to imputation can realistically capture regional variation in forest composition. We expect the imputation map will be valuable for several regional forest studies and that the approach could be successfully used in other regions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Duveneck, Matthew J.; Thompson, Jonathan R.] Harvard Univ, Harvard Forest, Petersham, MA 01366 USA. [Wilson, B. Tyler] USDA, Forest Serv, Forest Inventory & Anal, No Res Stn, St Paul, MN USA. RP Duveneck, MJ (reprint author), Harvard Univ, Harvard Forest, Petersham, MA 01366 USA. EM mduveneck@gmail.com FU National Science Foundation Harvard Forest Long Term Ecological Research Program [NSF-DEB 12-37491]; Scenarios Society and Solutions Research Coordination Network [NSF-DEB-13-38809] FX This research was supported in part by the National Science Foundation Harvard Forest Long Term Ecological Research Program (Grant No. NSF-DEB 12-37491) and the Scenarios Society and Solutions Research Coordination Network (Grant No. NSF-DEB-13-38809). This research was also made possible by MOU (14-MU-11242305-025) between Harvard University and USFS Northern Research Station, to use actual FIA plot locations in our analysis. Our sincere thanks to Brett Butler for his help developing this MOU. We also thank Sofie McComb and Luca Morreale for research assistance, and two anonymous reviewers for valuable feedback on an earlier version of this manuscript. NR 51 TC 1 Z9 1 U1 6 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2015 VL 347 BP 107 EP 115 DI 10.1016/j.foreco.2015.03.016 PG 9 WC Forestry SC Forestry GA CI2LR UT WOS:000354579600010 ER PT J AU Rhoades, CC Fornwalt, PJ Paschke, MW Shanklin, A Jonas, JL AF Rhoades, Charles C. Fornwalt, Paula J. Paschke, Mark W. Shanklin, Amber Jonas, Jayne L. TI Recovery of small pile burn scars in conifer forests of the Colorado Front Range SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fuel management; Forest restoration; Soil disturbance; Fire effects; Burn severity; Prescribed fire ID SOIL-NITROGEN AVAILABILITY; SLASH; FIRE; RESTORATION; DYNAMICS; REMOVAL; QUALITY; SITES AB The ecological consequences of slash pile burning are a concern for land managers charged with maintaining forest soil productivity and native plant diversity. Fuel reduction and forest health management projects have created nearly 150,000 slash piles scheduled for burning on US Forest Service land in northern Colorado. The vast majority of these are small piles (<5 m diameter). Similar to larger piles, we found that burning small piles had significant immediate effects on soil nutrients and physical and chemical properties and native plant cover. To evaluate the need to rehabilitate small piles and compare the effectiveness of treatment options, we examined soil and plant responses to treatments designed to alter soil nutrients, moisture and temperature and to increase seed availability. We compared four surface treatments (soil scarification, woodchip mulch, tree branch mulch, untreated scars), with and without addition of a native seed mixture. Natural recovery and treatment effects were examined for 2.5 years after pile burning at 19 conifer forest sites along the Colorado Front Range. Woodchip mulch had dramatic effects on soil moisture, temperature, decomposition and inorganic soil N compared to the other treatments, untreated scars or unburned areas; woodchip mulch also suppressed plant establishment. Seeding increased total native species richness as expected, but had marginal effects on forb cover and no effect on graminoid cover. Soil N availability and plant cover did not differ from unburned areas in the absence of surface or seeding treatments within two years of pile burning. Neither reduced seed availability nor altered soil properties following burning hindered revegetation of these small burn scars by native herbaceous plants. Our findings indicate that rehabilitation may not be required for small burn pile scars except in sensitive areas, such as those with water quality and invasive plant concerns. Published by Elsevier B.V. C1 [Rhoades, Charles C.; Fornwalt, Paula J.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Paschke, Mark W.; Shanklin, Amber; Jonas, Jayne L.] Colorado State Univ, Forest & Rangeland Stewardship Dept, Ft Collins, CO 80523 USA. RP Rhoades, CC (reprint author), US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. EM crhoades@fs.fed.us FU US Forest Service; Region 2 Burned Areas Rehabilitation and Native Plants Programs, Boulder County Parks and Open Space; Colorado Agriculture Experiment Station FX We gratefully acknowledge financial support from the US Forest Service, Region 2 Burned Areas Rehabilitation and Native Plants Programs, Boulder County Parks and Open Space, and the Colorado Agriculture Experiment Station. Eric Schroder and James White of the Arapaho Roosevelt National Forests and Claire DeLeo and Chad Julian of the Boulder County Parks and Open Space helped locate potential study sites and install rehabilitation treatments. Jenny Ventker helped establish the study sites and install treatments. Thanks to Brett Wolk, Derek Pierson and numerous students for assistance with field and laboratory work Susan Miller and three anonymous reviewers provided comments that helped clarify the manuscript. NR 38 TC 5 Z9 5 U1 4 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2015 VL 347 BP 180 EP 187 DI 10.1016/j.foreco.2015.03.026 PG 8 WC Forestry SC Forestry GA CI2LR UT WOS:000354579600017 ER PT J AU Willis, JL Walters, MB Gottschalk, KW AF Willis, John L. Walters, Michael B. Gottschalk, Kurt W. TI Scarification and gap size have interacting effects on northern temperate seedling establishment SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Scarification; Germination; Light; Gaps; Diversity; Seedling ID EASTERN-UNITED-STATES; WHITE-TAILED DEER; LATE-SUCCESSIONAL FORESTS; HEMLOCK-HARDWOOD FORESTS; SINGLE-TREE SELECTION; OLD-GROWTH; YELLOW BIRCH; NATURAL REGENERATION; SPECIES-DIVERSITY; NORTHEASTERN MINNESOTA AB After decades focused on promoting economically valuable species, management of northern temperate forests has increasingly become focused on promoting tree species diversity. Unfortunately, many formerly common species that could contribute to diversity including yellow birch (Betula alleghaniensis Britton.), paper birch (Betula papyrifera Marsh), eastern hemlock (Tsuga canadensis L.), and eastern white pine (Pinus strobus L) are now uncommon in the seedling layer, raising concerns about our ability to use these species to increase diversity. In this study, two related seed addition experiments conducted in 45 variably-sized harvest gaps (unharvested to 6500 m(2)) in adjacent mesic northern hardwood stands, Emmet County, Michigan, USA were used to investigate mechanisms potentially limiting seedling recruitment. The first experiment examined the influence of light (i.e. harvest gap size), competing vegetation, and deer browsing on seedling survival for three years in a 2 x 2 factorial, where subplots were unfenced or fenced to exclude deer, unclipped or clipped to control competing vegetation, and located across the gradient of gap sizes. The second experiment explored the influence of scarification, light, and competing vegetation on germination and subsequent survival for 2 years in a 2 x 2 factorial, in subplots that were unscarified or scarified to remove litter, unclipped or clipped to control competing vegetation, and located across the gradient of gap sizes. Eastern hemlock, paper birch, and yellow birch, all smaller-seeded species, were 12, 17, and 95 times more abundant in scarified plots compared to unscarified plots. In contrast, white pine, the largest-seeded species, was unaffected by scarification and had low overall germination. Shade tolerant hemlock and shade intolerant paper birch germinated at higher densities in lower light, smaller harvest gap environments, while both mid-tolerant species, white pine and yellow birch, were unaffected by light. Each species' initial survival significantly increased with increasing light availability, and with the exception of yellow birch, each species also survived at a significantly higher rate with increasing light availability. Paper birch and hemlock third year survival also increased with increasing light. By the end of the third growing season, only paper birch survival was negatively impacted by competition from vegetation and no species were affected by exposure to deer browse pressure. At the conclusion of the study, large group selection gaps (24-50 m diameter) contained the highest density of each species except white pine, suggesting that large group selection gaps may provide the best opportunity for reestablishing this particular group of species in the seedling layer. (C) 2015 Elsevier B.V. All rights reserved. C1 [Willis, John L.] Mississippi State Univ, Dept Forestry, Mississippi State, MS 39762 USA. [Walters, Michael B.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Gottschalk, Kurt W.] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. RP Willis, JL (reprint author), Mississippi State Univ, Dept Forestry, 361 Thompson Hall,775 Stone Blvd, Mississippi State, MS 39762 USA. EM jw2905@msstate.edu; mwalters@msu.edu; kgottschalk@fs.fed.us FU Michigan Department of Natural Resources; United States Forest Service FX This project was funded by the Michigan Department of Natural Resources and the United States Forest Service. The authors would like to thank Evan Farinosi, Ben Daly, Emily Standen, Kaitlyn Snarski, and Casey Koleski for all their efforts in the field. We also thank Dr. Lars Brudvig, Dr. Rich Kobe, and Dr. David Rothstein for their helpful editorial comments. We also thank the two anonymous reviewers for their helpful comments. Finally we thank Sasha and Odo for all their indispensable efforts. NR 100 TC 8 Z9 9 U1 12 U2 71 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2015 VL 347 BP 237 EP 246 DI 10.1016/j.foreco.2015.02.026 PG 10 WC Forestry SC Forestry GA CI2LR UT WOS:000354579600023 ER PT J AU Gava, D Souza, CK Schaefer, R Vincent, AL Cantao, ME Coldebella, A Ciacci-Zanella, JR AF Gava, Danielle Souza, Carine K. Schaefer, Rejane Vincent, Amy L. Cantao, Mauricio E. Coldebella, Arlei Ciacci-Zanella, Janice R. TI A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4 SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Taq Man qPCR; Porcine parvovirus 4; Swine ID GENETIC-ANALYSIS; IDENTIFICATION; BOCAVIRUSES; PIGS AB Porcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TagManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 x 10(1) DNA copies/mu L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98-99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds. (C) 2015 Elsevier B.V. All rights reserved. C1 [Gava, Danielle; Schaefer, Rejane; Cantao, Mauricio E.; Coldebella, Arlei; Ciacci-Zanella, Janice R.] Embrapa Swine & Poultry, Hlth Anim Lab, BR-89700000 Concordia, SC, Brazil. [Souza, Carine K.] Univ Fed Rio Grande do Sul, Porto Alegre, RS, Brazil. [Vincent, Amy L.] USDA ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, Ames, IA USA. RP Gava, D (reprint author), Embrapa Swine & Poultry, Hlth Anim Lab, BR 153,Km 110, BR-89700000 Concordia, SC, Brazil. EM danielle.gava@embrapa.br RI Coldebella, Arlei/A-4488-2013; Gava, Danielle/D-7230-2013; Zanella, Janice/C-3632-2014 OI Coldebella, Arlei/0000-0003-1979-6021; Gava, Danielle/0000-0002-1917-2633; FU EMBRAPA; CNPq/Brazil FX The authors thank Camila Sa Rocha and Giseli Aparecida Ritterbusch for samples collection and Neide Lisiane Simon for technical assistance. We also thank Dr. Andrew Cheung for PPV4 genomic sequence. Funding was provided by EMBRAPA, and CNPq/Brazil. NR 18 TC 2 Z9 2 U1 2 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD JUL PY 2015 VL 219 BP 14 EP 17 DI 10.1016/j.jviromet.2015.03.011 PG 4 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA CI8RX UT WOS:000355040500003 PM 25813599 ER PT J AU Berner, DK Smallwood, EL Cavin, CA McMahon, MB Thomas, KM Luster, DG Lagopodi, AL Kashefi, JN Mukhina, Z Kolomiets, T Pankratova, L AF Berner, D. K. Smallwood, E. L. Cavin, C. A. McMahon, M. B. Thomas, K. M. Luster, D. G. Lagopodi, A. L. Kashefi, J. N. Mukhina, Zhanna Kolomiets, Tamara Pankratova, Lyubov TI Asymptomatic systemic disease of Canada thistle (Cirsium arvense) caused by Puccinia punctiformis and changes in shoot density following inoculation SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Canada thistle; Chemiluminescence western slot blot; Cirsium arvense; Epidemics; Rust fungus; Systemic disease ID ALLELOPATHIC ACTIVITY; L SCOP; TELIOSPORES; RUST; GERMINATION; MANAGEMENT; HERBICIDES; INFECTION; PATHOGENS; BIOLOGY AB Canada thistle (Cirsium arvense) is one of the worst weeds in temperate areas of the world. A rust fungus, Puccinia punctiformis, was first proposed as a biological control agent for C. arvense in 1893. The rust causes systemic disease which ultimately kills C. arvense plants. In 2013 it was demonstrated in four countries, that inoculation of C. arvense rosettes in the fall with ground telia-bearing leaves can initiate epidemics of systemic rust disease with an average of 28% of inoculated rosettes producing a systemically diseased shoot the following spring. Other rosettes that emerged near inoculation points in spring were stunted and appeared diseased. To determine whether other rosettes were diseased, a chemiluminescence western slot blot test, applying polyclonal antibodies raised against P. punctiformis antigens, was developed to detect the fungus in roots. Rosettes were inoculated with telia-bearing leaves in the fall in Maryland, USA and Veroia, Greece. Roots of asymptomatic rosettes that emerged adjacent to inoculation points the following spring were tested for the presence of the fungus with the slot blot test. Rosettes that had diseased shoots were recorded. Based on the slot blot tests, 50-60% of the asymptomatic rosettes adjacent to inoculation points were positive for presence of the rust and likely to be systemically diseased. To demonstrate that systemic disease leads to C. arvense decline, C. arvense shoot densities were measured annually at 10 sites, in three countries, that had been inoculated with teliabearing leaves in the fall between 2008 and 2012. Changes in C. arvense shoot densities over time were calculated. Average reductions in C. arvense density across the 10 sites were 43.1 +/- 10.0% at 18 months after inoculation, 63.8 +/- 8.0% at 30 months after inoculation, and 80.9 +/- 16.5% at 42 months after inoculation, and 72.9 +/- 27.2% at 54 months after inoculation; the 54 month reduction was, however, based on only two sites. Published by Elsevier Inc. C1 [Berner, D. K.; Smallwood, E. L.; Cavin, C. A.; McMahon, M. B.; Thomas, K. M.; Luster, D. G.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Lagopodi, A. L.] Aristotle Univ Thessaloniki, Sch Agr, GR-54124 Thessaloniki, Greece. [Kashefi, J. N.] ARS, USDA, European Biol Control Lab, GR-54623 Thessaloniki, Greece. [Mukhina, Zhanna] All Russia Res Inst Biol Means Plant Protect, Krasnodar 350039, Russia. [Kolomiets, Tamara; Pankratova, Lyubov] All Russia Res Phytopathol Inst, VNIIF, B Vazemy 143050, Moscow Region, Russia. RP Berner, DK (reprint author), ARS, USDA, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM dkber584@gmail.com FU International Science and Technology Center - USDA/ARS Office of International Research Programs [3289]; U.S. Department of State; USDA, ARS, European Biological Control Laboratory, Montpellier, France FX Research in Russia was supported by International Science and Technology Center Grant #3289 funded by the USDA/ARS Office of International Research Programs and the U.S. Department of State. Research in Greece was supported by USDA, ARS, European Biological Control Laboratory, Montpellier, France. NR 44 TC 1 Z9 1 U1 3 U2 16 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2015 VL 86 BP 28 EP 35 DI 10.1016/j.biocontrol.2015.02.006 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CI2DD UT WOS:000354554200005 ER PT J AU Schisler, DA Boehm, MJ Paul, PA Rooney, AP Dunlap, CA AF Schisler, David A. Boehm, Michael J. Paul, Pierce A. Rooney, Alejandro P. Dunlap, Chris A. TI Reduction of Fusarium head blight using prothioconazole and prothioconazole-tolerant variants of the Fusarium head blight antagonist Cryptococcus flavescens OH 182.9 SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Chemical control; Fungicide tolerant; Strain improvement; Integrated disease management; Fusarium head blight ID BIOLOGICAL-CONTROL; BIOCONTROL AGENT; MYCOTOXIN CONTAMINATION; DEOXYNIVALENOL CONTENT; GIBBERELLA-ZEAE; NODAENSIS OH-182.9; SEQUENCE ALIGNMENT; INTEGRATED CONTROL; BOTRYTIS-CINEREA; F-GRAMINEARUM AB Combining the biological control agent Cryptococcus flavescens OH 182.9 (NRRL Y-30216) with a triazole fungicide has potential for significantly reducing Fusarium head blight (FHB) but is complicated by yeast sensitivity to the chemical. Protracted exposure of strain OH 182.9 to increasing concentrations of prothioconazole (PTC) during growth in liquid culture resulted in stable variants with doubling times of 135-174 min in the presence of 50 ppm prothioconazole compared to 228 min for the progenitor strain. Prothioconazole-tolerant (PTCT) variants were confirmed to be cospecific with the progenitor strain using Biolog (TM) YT Microplate (TM) and phylogenetic analysis. In greenhouse tests, PTCT variant OH 182.9 4C reduced head blight severity by 83% compared to 36% for the wild type strain (P <= 0.05, Bonferroni mean separation). Disease reductions incited by other variants were not significant. Four PTCT variants, PTC applied as Proline (R) at label rate, and combinations of PTCT variants and fungicide significantly reduced FHB severity and incidence (P <= 0.05). In two field trials on susceptible winter wheat, OH 182.9 3C and OH 182.9 4C reduced severity by 34% and 42%, respectively, compared to the control (P <= 0.05) in one trial and 21% and 24%, respectively, in the other (not significantly different at P <= 0.05). In comparison, strain OH 182.9 did not reduce FHB severity consistently. For both field trials on two wheat varieties, treating flowering wheat heads with PTC and a PTCT variant resulted in the greatest numerical reduction of FHB severity, incidence, and DON relative to other treatments. However, combining PTCT variants with PTC did not significantly enhance treatment efficacy vs PTC alone. Published by Elsevier Inc. C1 [Schisler, David A.; Rooney, Alejandro P.; Dunlap, Chris A.] USDA ARS, NCAUR, Peoria, IL 61604 USA. [Boehm, Michael J.] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. [Paul, Pierce A.] Ohio State Univ, Dept Plant Pathol, OARDC, Wooster, OH 44691 USA. RP Schisler, DA (reprint author), USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM David.Schisler@ars.usda.gov FU U.S. Wheat and Barley Scab Initiative; state and federal funds FX The assistance of Jennifer Sloan, Maggie Hammar, Todd Hicks, Joe Rimelspach, Matthew Wallhead, William Bardall and Karlten Austin in conducting experiments is greatly appreciated as are the efforts of Dr. Yanhong Dong, University of Minnesota, in conducting the DON analysis. Studies were made possible, in part, by a grant from the U.S. Wheat and Barley Scab Initiative. Salaries and additional research support for P.A. Paul and M.J. Boehm were provided by state and federal funds to the Ohio Agricultural Research and Development Center, The Ohio State University. NR 71 TC 0 Z9 1 U1 3 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2015 VL 86 BP 36 EP 45 DI 10.1016/j.biocontrol.2015.04.002 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CI2DD UT WOS:000354554200006 ER PT J AU Feng, ZZ Paoletti, E Bytnerowicz, A Harmens, H AF Feng, Zhaozhong Paoletti, Elena Bytnerowicz, Andrzej Harmens, Harry TI Ozone and plants SO ENVIRONMENTAL POLLUTION LA English DT Editorial Material DE Ozone ID GROUND-LEVEL OZONE; CHINA C1 [Feng, Zhaozhong] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China. [Paoletti, Elena] IPSP CNR, I-50019 Florence, Italy. [Bytnerowicz, Andrzej] US Forest Serv, Pacific NW Res Stn, Riverside, CA USA. [Harmens, Harry] Environm Ctr Wales, Ctr Ecol & Hydrol, Bangor LL57 2UW, Gwynedd, Wales. RP Feng, ZZ (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Urban & Reg Ecol, Shuangqing Rd 18, Beijing 100085, Peoples R China. EM fzz@rcees.ac.cn RI Harmens, Harry/J-2887-2012; OI Harmens, Harry/0000-0001-8792-0181; Paoletti, Elena/0000-0001-5324-7769 NR 15 TC 4 Z9 6 U1 8 U2 45 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUL PY 2015 VL 202 BP 215 EP 216 DI 10.1016/j.envpol.2015.02.004 PG 2 WC Environmental Sciences SC Environmental Sciences & Ecology GA CI2MO UT WOS:000354581900026 PM 25681054 ER PT J AU Cornelius, ML Osbrink, WLA AF Cornelius, Mary L. Osbrink, Weste L. A. TI Natural resistance of exotic wood species to the Formosan subterranean termite (Isoptera: Rhinotermitidae) SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Coptotermes formosanus; Termite survival; Wood consumption; Wood preservatives ID COPTOTERMES-FORMOSANUS; RETICULITERMES-FLAVIPES; ANTITERMITIC ACTIVITY; ANTIFEEDANT ACTIVITY; ESSENTIAL OILS; ALLELOCHEMICAL RESISTANCE; FEEDING RESPONSES; SOUND WOOD; HEARTWOOD; EXTRACTS AB The purpose of this study was to evaluate survival and wood consumption of the Formosan subterranean termite, Coptotermes formosanus Shiraki, on ten different species of wood used as commercial lumber. Six of the wood species had natural resistance to termites and caused an average of >75% mortality. Southern yellow pine and spruce were the most palatable and teak was the most resistant of the wood tested. A test was also conducted to compare survival of termites on resistant wood with survival under starvation conditions after three and six weeks. After six weeks, survival of termites on teak was significantly lower than in the starvation control, suggesting that at least some of the termite mortality on teak may have been due to toxicity. Toxic chemical components of teak hold the most promise as wood preservatives. Published by Elsevier Ltd. C1 [Cornelius, Mary L.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Osbrink, Weste L. A.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Cornelius, ML (reprint author), ARS, USDA, Beltsville Agr Res Ctr, 10300 Baltimore Ave,Bld 007,Rm 313, Beltsville, MD 20705 USA. EM mary.cornelius@ars.usda.gov NR 36 TC 0 Z9 0 U1 2 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD JUL PY 2015 VL 101 BP 8 EP 11 DI 10.1016/j.ibiod.2015.03.016 PG 4 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA CI2ML UT WOS:000354581600002 ER PT J AU Hohenschuh, W Hector, R Murthy, GS AF Hohenschuh, William Hector, Ronald Murthy, Ganti S. TI A dynamic flux balance model and bottleneck identification of glucose, xylose, xylulose co-fermentation in Saccharomyces cerevisiae SO BIORESOURCE TECHNOLOGY LA English DT Article; Proceedings Paper CT International Conference on Emerging Trends in Biotechnology (ICETB) CY 2014 CL New Delhi, INDIA SP Biotech Res Soc, Jawaharlal Nehru Univ, Sch Environm Sci DE Xylose; Cellulosic ethanol; Fermentation; Pentose; Flux balance analysis ID PENTOSE-PHOSPHATE PATHWAY; TECHNOECONOMIC EVALUATION; BIOETHANOL PRODUCTION; HXT GENES; YEAST; ETHANOL; TRANSPORTERS; ISOMERASE; GROWTH AB A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further experiments showed xylulose transport through the HXT family of non-specific glucose transporters. A genome scale flux balance model was developed which included an improved variable sugar uptake constraint controlled by HXT expression. Model predictions closely matched experimental xylulose utilization rates suggesting the combination of transport and xylulokinase constraints is sufficient to explain xylulose utilization limitation in S. cerevisiae. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Hohenschuh, William; Murthy, Ganti S.] Oregon State Univ, Corvallis, OR 97331 USA. [Hector, Ronald] ARS, USDA, NCAUR, Washington, DC USA. RP Murthy, GS (reprint author), 116 Gilmore Hall, Corvallis, OR 97331 USA. EM MurthyG@ONID.OregonState.EDU RI Murthy, Ganti /P-5272-2015 NR 20 TC 3 Z9 3 U1 1 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUL PY 2015 VL 188 BP 153 EP 160 DI 10.1016/j.biortech.2015.02.015 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CH2YF UT WOS:000353890800023 PM 25791332 ER PT J AU Turner, KE Cassida, KA Zerby, HN Brown, MA AF Turner, K. E. Cassida, K. A. Zerby, H. N. Brown, M. A. TI Carcass parameters and meat quality in meat-goat kids finished on chicory, birdsfoot trefoil, or red clover pastures SO MEAT SCIENCE LA English DT Article DE Goat; Pasture-finished; Chicory; Birdsfoot trefoil; Red clover; Carcass; Meat quality ID FATTY-ACID-COMPOSITION; GRAZING LOTUS-CORNICULATUS; LUCERNE MEDICAGO-SATIVA; SULLA HEDYSARUM-CORONARIUM; TRIFOLIUM-REPENS PASTURE; CICHORIUM-INTYBUS; CONDENSED TANNINS; SESQUITERPENE LACTONES; ORCHARDGRASS PASTURES; SPANISH GOATS AB This study was conducted during the 2009 and 2010 grazing seasons to assess carcass parameters and chevon (goat meat) quality when meat-goat kids (n = 72) were finished on pastures of red clover (Trifolium pratense L.; RCL), birdsfoot trefoil (Lotus corniculatus L; BFT), or chicory (Cichorium intybus L.; CHIC). Final live weight (P < 0.05) and carcass weight (P = 0.10) were greater when goats were finished on RCL compared to CHIC with BET being intermediate. Ribeye area, backfat thickness, body wall thickness, internal fat score, and leg score were not different (P > 0.10) among treatments when adjusted for the covariate of carcass weight. Finishing meat-goat kids on RCL, BET, or CHIC impacted concentrations of fatty acids (FAs) 18:1 trans-10, 18:1 cis-11, 18:2, 18:3 polyunsaturated fatty acids (PUFAs), omega-6, omega-3, and PUFA:saturated fatty acid ratio in longissimus lumborum samples. Finishing meat-goat kids on CHIC, RCL, or BFT pastures produced carcass weights acceptable for most ethnic markets in the USA. Published by Elsevier Ltd. C1 [Turner, K. E.; Brown, M. A.] ARS, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. [Cassida, K. A.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Zerby, H. N.] Ohio State Univ, Dept Anim Sci, Meat Sci, Columbus, OH 43210 USA. RP Turner, KE (reprint author), ARS, USDA, Grazinglands Res Lab, 7207 West Cheyenne St, El Reno, OK 73036 USA. EM Ken.Turner@ars.usda.gov NR 62 TC 3 Z9 3 U1 4 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1740 EI 1873-4138 J9 MEAT SCI JI Meat Sci. PD JUL PY 2015 VL 105 BP 68 EP 74 DI 10.1016/j.meatsci.2015.03.005 PG 7 WC Food Science & Technology SC Food Science & Technology GA CH6NF UT WOS:000354152600011 PM 25817803 ER PT J AU Wu, XM Huang, YW Park, B Tripp, RA Zhao, YP AF Wu, Xiaomeng Huang, Yao-Wen Park, Bosoon Tripp, Ralph A. Zhao, Yiping TI Differentiation and classification of bacteria using vancomycin functionalized silver nanorods array based surface-enhanced Raman spectroscopy and chemometric analysis SO TALANTA LA English DT Article DE Surface enhanced Raman scattering; Bacteria detection; Silver nanorods; Vancomycin; Chemometric analysis ID FOODBORNE PATHOGENIC BACTERIA; LABEL-FREE DETECTION; SCATTERING SERS; NANOPARTICLES; DISCRIMINATION; IDENTIFICATION; SENSITIVITY; MOLECULES; CELLS AB Twenty seven different bacteria isolates from 12 species were analyzed using intrinsic surface-enhanced Raman scattering (SERS) spectra with recently developed vancomycin coated silver nanorod (VAN AgNR) substrates. The VAN AgNR substrates could generate reproducible SERS spectra of the bacteria with little to no interference from the environment or bacterial by-products as compared to the pristine substrates. By taking advantage of the structural composition of the cellular wall which varies from species to species, the differentiation of bacterial species is demonstrated by using chemometric analyses on those spectra. A second chemometric analysis step within the species cluster is able to differentiate serotypes and strains. The spectral features used for serotype differentiation arises from the surface proteins, while Raman peaks from adenine dominate the differentiation of strains. In addition, due to the intrinsic structural differences in the cell walls, the SERS spectra can distinguish Gram-positive from Gram-negative bacteria with high sensitivity and specificity, as well as 100% accuracy on predicting test samples. Our results provide important insights for using SERS as a bacterial diagnostic tool and further guide the design of a SERS-based detection platform. (C) 2015 Elsevier B.V. All rights reserved. C1 [Wu, Xiaomeng; Huang, Yao-Wen] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. [Wu, Xiaomeng; Zhao, Yiping] Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA. [Park, Bosoon] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA. [Tripp, Ralph A.] Univ Georgia, Dept Infect Dis, Athens, GA 30602 USA. [Zhao, Yiping] Univ Georgia, Dept Phys & Astron, Athens, GA 30602 USA. RP Zhao, YP (reprint author), Univ Georgia, Dept Phys & Astron, Athens, GA 30602 USA. EM zhaoy@physast.uga.edu RI Zhao, Yiping/A-4968-2008 FU USDA CSREES [2009-35603-05001]; National Science Foundation [CBET-1064228]; University of Georgia College of Agricultural and Environmental Sciences Experiment Station FX This work was partly supported by USDA CSREES Grant #2009-35603-05001, National Science Foundation under contract number CBET-1064228, and University of Georgia College of Agricultural and Environmental Sciences Experiment Station. The authors would like to thank Dr. Rene Alvarez and Dr. Alexander J. Burdette for their help throughout the project and the preparation of the manuscript. The authors would also like to thank Dr. Arthur Hinton, Jr. for providing the bacterial strains and Dr. Nasreen Bano for bacterial sample preparation. NR 40 TC 8 Z9 8 U1 11 U2 128 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0039-9140 EI 1873-3573 J9 TALANTA JI Talanta PD JUL 1 PY 2015 VL 139 BP 96 EP 103 DI 10.1016/j.talanta.2015.02.045 PG 8 WC Chemistry, Analytical SC Chemistry GA CH2LU UT WOS:000353857500014 PM 25882413 ER PT J AU Liu, SX Singh, M Wayman, AE Hwang, HS Fhaner, M AF Liu, Sean X. Singh, Mukti Wayman, Ashley E. Hwang, Hong-Sik Fhaner, Matthew TI Reduction of omega-3 oil oxidation in stable emulsion of caseinate-omega-3 oil-oat beta-glucan SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Omega-3 oils; Emulsion; Caseinate; Beta glucan; Lipid oxidation ID LIPID OXIDATION; INHIBITION; PRODUCTS AB Lipid oxidation of omega-3 fatty acids has posed a serious challenge for incorporating heart-healthy oil products into healthful foods and beverages. In this study, plant and marine based omega-3 oils were dispersed into sodium caseinate dispersions and then mixed with purified heart-healthy oat gum comprised of 97% beta-glucan in a homogenizer under room temperature. The stable emulsions underwent shelf life tests to assess their stabilities and oxidative activities. Various preparations of omega-3 oil-in-water emulsions with 10mL oil/100mLstabilized with sodium caseinate ranging from 0 to 3g caseinate/100g sample and beta-glucan ranging from 0 to 0.3g beta-glucan/100g sample were formed. The physical properties such as creaming index, particle size, and viscosity of the emulsions were measured. The lipid oxidation was measured as lipid hydroperoxide concentration and reduction of lipid oxidation was observed under shelf-life stress tests at 28 degrees C. The fatty acids of the oils were also measured using gas chromatography. Omega-3 fatty acids compositional changes were observed over the shelf life tests. It was found that caseinate helped reduce the oxidation of the oils in general and there is no significant impact of beta-glucan on oxidation. Addition of caseinate and beta-glucan in the emulsions slightly increased both particle size and viscosity. Published by Elsevier Ltd. C1 [Liu, Sean X.; Singh, Mukti; Wayman, Ashley E.; Hwang, Hong-Sik; Fhaner, Matthew] USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, Peoria, IL 61604 USA. RP Liu, SX (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Sean.liu@ars.usda.gov NR 22 TC 1 Z9 1 U1 1 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUL PY 2015 VL 62 IS 2 BP 1083 EP 1090 DI 10.1016/j.lwt.2015.02.012 PG 8 WC Food Science & Technology SC Food Science & Technology GA CG1CD UT WOS:000353009700021 ER PT J AU Zhang, Y Pan, ZL Venkitasamy, C Ma, HL Li, YL AF Zhang, Yin Pan, Zhongli Venkitasamy, Chandrasekar Ma, Haile Li, Yunliang TI Umami taste amino acids produced by hydrolyzing extracted protein from tomato seed meal SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Umami taste; Amino acids; Glutamic acid; Aspartic acid; Tomato seed meal ID RESPONSE-SURFACE METHODOLOGY; ENZYMATIC-HYDROLYSIS; BIOLOGICAL EVALUATION; FUNCTIONAL-PROPERTIES; PARTICLE-SIZE; OPTIMIZATION; TEMPERATURE; COLLAGEN; GLUTEN AB Enzymatic hydrolysis was performed for extracting protein to prepare umami taste amino acids from defatted tomato seed meal (DTSM) which is a by-product of tomato processing. Papain was used as an enzyme for the hydrolysis of DTSM. The particle size distribution of DTSM, protein concentration and free amino acids of the hydrolysate were analyzed. Response surface methodology was used in the design of experiments and analysis of results. The protein extraction ratio of greater than 85.64% was obtained when the DTSM with particle size <= 0.25 mm was hydrolyzed by papain for 5 h. The optimum extraction conditions to produce maximum concentration of umami taste amino acids were enzyme activity of 18.22%; pH of 3; hydrolysis temperature of 40 degrees C; and hydrolysis time of 6 h, which resulted in glutamic acid and aspartic acid concentrations of 727.6 mu g/mL and 149.9 mu g/mL, respectively. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Zhang, Yin] Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Peoples R China. [Zhang, Yin; Pan, Zhongli; Venkitasamy, Chandrasekar] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli] USDA ARS WRRC, Hlth Proc Foods Res Unit, Albany, CA 94710 USA. [Ma, Haile; Li, Yunliang] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China. [Ma, Haile] Jiangsu Prov Res Ctr Bioproc & Separat Engn Agrip, Zhenjiang 212013, Jiangsu, Peoples R China. RP Pan, ZL (reprint author), USDA ARS, Processed Foods Res Unit, West Reg Res Ctr, 800 Buchanan St, Berkeley, CA 94710 USA. EM zlpan@ucdavis.edu NR 31 TC 3 Z9 3 U1 6 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUL PY 2015 VL 62 IS 2 BP 1154 EP 1161 DI 10.1016/j.lwt.2015.02.003 PG 8 WC Food Science & Technology SC Food Science & Technology GA CG1CD UT WOS:000353009700031 ER PT J AU Alfaro, L Hayes, D Boeneke, C Xu, ZM Bankston, D Bechtel, PJ Sathivel, S AF Alfaro, Luis Hayes, Daniel Boeneke, Charles Xu, Zhimin Bankston, David Bechtel, Peter J. Sathivel, Subramaniam TI Physical properties of a frozen yogurt fortified with a nano-emulsion containing purple rice bran oil SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Frozen yogurt; Antioxidants; Functional foods; Physicochemical properties; Rice bran oil ID LACTIC-ACID BACTERIA; ICE-CREAM; RHEOLOGICAL PROPERTIES; TEMPERATURE; OXIDATION; PROTEIN; DEGRADATION; QUALITY; FIBER; FOOD AB The objectives of this study were to develop and evaluate a frozen yogurt (FY) fortified with a nanoemulsion containing purple rice bran oil (NPRBO). NPRBO with fat droplets size range of 150-300 nm was mixed with the FY ingredients to produce a frozen yogurt containing NPRBO (FYNRO). Plain frozen yogurt (PFY), frozen yogurt with sodium caseinate (FYSC) and FYNRO had similar hardness. The apparent viscosity of the FYNRO mix was similar to the PFY mix with values of 0.19 and 0.17 Pa.s, respectively. The FYNRO micrograph showed a more compact and dense structure compared to FYSC and PFY. This study demonstrated that FY could be fortified with NPRBO. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Alfaro, Luis; Xu, Zhimin; Bankston, David; Sathivel, Subramaniam] Louisiana State Univ, Ctr Agr, Sch Nutr & Food Sci, Baton Rouge, LA 70803 USA. [Hayes, Daniel; Sathivel, Subramaniam] Louisiana State Univ, Ctr Agr, Dept Biol & Agr Engn, Baton Rouge, LA 70803 USA. [Boeneke, Charles] Louisiana State Univ, Ctr Agr, Dept Anim Sci, Baton Rouge, LA 70803 USA. [Bechtel, Peter J.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Sathivel, S (reprint author), Louisiana State Univ, Ctr Agr, Sch Nutr & Food Sci, Baton Rouge, LA 70803 USA. EM ssathivel@agcenter.lsu.edu NR 48 TC 1 Z9 1 U1 4 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUL PY 2015 VL 62 IS 2 BP 1184 EP 1191 DI 10.1016/j.lwt.2015.01.055 PG 8 WC Food Science & Technology SC Food Science & Technology GA CG1CD UT WOS:000353009700035 ER PT J AU Nam, S Slopek, R Condon, BD Sawhney, P AF Nam, Sunghyun Slopek, Ryan Condon, Brian D. Sawhney, Paul TI Effect of polyester blends in hydroentangled raw and bleached cotton nonwoven fabrics on the adsorption of alkyl-dimethyl-benzyl-ammonium chloride SO TEXTILE RESEARCH JOURNAL LA English DT Article DE raw cotton; hydroentangled nonwoven; wipes; adsorption; kinetics; isotherm; first-order equation; KASRA model ID CELLULOSE FIBERS; ELECTROKINETIC PROPERTIES; SURFACTANT ADSORPTION; SORPTION; INTERFACE; KINETICS; CHARGE; MODEL AB The adsorption kinetics and isotherms of alkyl-dimethyl-benzyl-ammonium chloride (ADBAC), a cationic surfactant commonly employed as an antimicrobial agent, on hydroentangled nonwoven fabrics (applicable for wipes) including raw cotton, bleached cotton, and their blends with polyester (PES) were studied at room temperature. The adsorption kinetics of ADBAC on all nonwoven fabrics was best described by the pseudo-first-order kinetic model. Unlike bleached cotton/PES blends, the equilibrium adsorption capacities of ADBAC on raw cotton/PES blends were enhanced in comparison with predictions based on the binary mixing rule. The adsorption rates for raw cotton, determined by the KASRA (kinetics of adsorption study in the regions with constant adsorption acceleration) model and Elovich equation, were significantly greater than those for bleached cotton, resulting in a rapid decrease of adsorption rates when blending with PES, which has a negligible interaction with ADBAC. This distinctive adsorption property of raw cotton was attributed to its unique surface characteristics induced by the hydroentangling process: retained pectin, partial removal of waxes, and surface fibrillation, which enhance electrostatic interaction, hydrophobic interaction, and accessible surface area to ADBAC, respectively. In adsorption isotherms, raw cotton/PES blends exhibited a non-linear decrease in maximum adsorption capacity and monolayer adsorption capacity, calculated by the Langmuir and Langmuir-type equations, respectively, as a function of PES blend ratio. C1 [Nam, Sunghyun; Slopek, Ryan; Condon, Brian D.; Sawhney, Paul] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Condon, BD (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM brian.condon@ars.usda.gov NR 39 TC 3 Z9 3 U1 0 U2 14 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 EI 1746-7748 J9 TEXT RES J JI Text. Res. J. PD JUL PY 2015 VL 85 IS 12 BP 1221 EP 1233 DI 10.1177/0040517514561918 PG 13 WC Materials Science, Textiles SC Materials Science GA CG9LL UT WOS:000353636600002 ER PT J AU Chen, TW Huang, LH Lai, GX Chen, GY AF Chen, Tuanwei Huang, Lihan Lai, Guoxin Chen, Guoying TI Inorganic arsenic in starchy roots, tubers, and plantain and assessment of cancer risk of sub-Saharan African populations SO FOOD CONTROL LA English DT Article DE Inorganic arsenic; Cancer risk; Root and tuber; Plantain; Cassava; Potato; Sweet potato; Yam; Aroid ID EPIDEMIOLOGIC EVIDENCE; EXPOSURE; RICE; SPECIATION; FOOD; QUANTIFICATION; CONTAMINATION; CONSUMPTION; BANGLADESH; DISEASE AB Starchy roots, tubers, and plantain (RTP) are the staple food in sub-Saharan Africa (SSA); and also important energy sources in Asia, Europe, and America. In this work, inorganic arsenic (iAs) in these crops was separated and enriched by solid phase extraction (SPE), and quantified by hydride generation-atomic fluorescence spectrometry (HG-AFS). Overall, iAs in these crops ranged from 0.9 to 14.1 ng g(-1) wet weight. Long-term cancer risk associated with iAs intake from these crops was assessed by Monte Carlo simulation based on iAs concentrations and historical consumption and population data. For 19 high RIP consuming SSA countries, life-time cancer risk was low with a mean target risk at 6.3 x 10(-5) and a margin of exposure at 72. Published by Elsevier Ltd. C1 [Chen, Tuanwei] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China. [Huang, Lihan; Chen, Guoying] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Lai, Guoxin] Meizhouwan Vocat Technol Coll, Dept Chem Engn, Putian 351254, Fujian, Peoples R China. RP Chen, GY (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM guoying.chen@ars.usda.gov RI Chen, Guoying/C-6800-2015 OI Chen, Guoying/0000-0001-9532-0696 FU Training Program of Fujian Excellent Talents in Universities (FETU), Fujian, China FX Bun-hong Lai is acknowledged for technical assistance. Guoxin Lai is grateful for the financial support from the Training Program of Fujian Excellent Talents in Universities (FETU), Fujian, China. NR 43 TC 1 Z9 1 U1 2 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUL PY 2015 VL 53 BP 104 EP 108 DI 10.1016/j.foodcont.2015.01.016 PG 5 WC Food Science & Technology SC Food Science & Technology GA CE9NW UT WOS:000352171700015 ER PT J AU Fang, T Huang, LH Liu, LJ Mei, F Chen, JQ AF Fang, Ting Huang, Lihan Liu, Lijun Mei, Fan Chen, Jinquan TI Mathematical modeling of growth of Salmonella spp. and spoilage microorganisms in raw oysters SO FOOD CONTROL LA English DT Article DE Mathematical model; Salmonella; Spoilage microorganisms; Oysters ID COASTAL WATERS; MARINE-ENVIRONMENT; SEAFOOD; TEMPERATURE; PREVALENCE; SEROVARS; TYPHIMURIUM; INFECTIONS; SHELLFISH; FISH AB The main objective of this study was to develop the primary and secondary models to describe the growth kinetics of Salmonella as well as background microorganisms in raw, shucked oysters. Samples, inoculated with a cocktail of two Salmonella serotypes, S. Typhimurium (CICC22956) and S. Enteritidis (CICC21482), were incubated at 4, 8, 12, 16, 20, 25, 30, 33, 37, 40, and 43 degrees C. Growth of Salmonella was observed at all temperatures, except at 4 degrees C. The background microorganisms grew at all temperatures. All growth curves clearly exhibited lag, exponential and stationary phases, and were analyzed using the Huang growth model. Three secondary models (Ratkowsky square-root, Huang square-root, and Cardinal parameter models) were compared for evaluating the effect of temperature on bacterial growth rates. Data analysis was performed using IPMP 2013, a free predictive microbiology software tool developed by the USDA ARS. The Cardinal parameters model underestimated the specific rates of the microorganisms at low temperatures. The Huang square-root model was more suitable than the Ratkowsky square-root model for describing the effect of temperature on growth of Salmonella, while the Ratkowsky square-root model, on the other hand, was more suitable for background microorganisms. For both Salmonella and background microorganisms, the logarithms of the lag phase were expressed as linear functions of the logarithms of specific growth rates. The results of this study can be used by the food retailers and regulatory agencies to estimate the microbial shelf-life of raw, shucked oysters. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Fang, Ting; Liu, Lijun; Mei, Fan; Chen, Jinquan] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350001, Peoples R China. [Huang, Lihan] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Fang, T (reprint author), Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350001, Peoples R China. EM fangting930@163.com FU Agriculture Research Service, United States Department of Agriculture; Fujian Agriculture and Forestry University; National Natural Science Foundation of China [31401597]; Fujian Provincial Department of Education Program [JA13112]; Fujian Provincial Natural Science Foundation [2013J01084] FX This work was under the cooperative agreement between the Agriculture Research Service, United States Department of Agriculture and Fujian Agriculture and Forestry University. The project was supported by National Natural Science Foundation of China (31401597), Fujian Provincial Department of Education Program (JA13112) and Fujian Provincial Natural Science Foundation (2013J01084). NR 31 TC 0 Z9 1 U1 8 U2 52 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUL PY 2015 VL 53 BP 140 EP 146 DI 10.1016/j.foodcont.2014.12.036 PG 7 WC Food Science & Technology SC Food Science & Technology GA CE9NW UT WOS:000352171700020 ER PT J AU Martens, B Lievens, H Colliander, A Jackson, TJ Verhoest, NEC AF Martens, Brecht Lievens, Hans Colliander, Andreas Jackson, Thomas J. Verhoest, Niko E. C. TI Estimating Effective Roughness Parameters of the L-MEB Model for Soil Moisture Retrieval Using Passive Microwave Observations From SMAPVEX12 SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Passive Active L-band Sensor (PALS); passive microwave remote sensing; roughness modeling; soil moisture ID L-BAND RADIOMETER; DISCHARGE PREDICTIONS; SURFACE-ROUGHNESS; OCEAN SALINITY; SIMPLEX-METHOD; CROP FIELDS; CALIBRATION; EMISSION; RADAR; SCALE AB Despite the continuing efforts to improve existing soil moisture retrieval algorithms, the ability to estimate soil moisture from passive microwave observations is still hampered by problems in accurately modeling the observed microwave signal. This paper focuses on the estimation of effective surface roughness parameters of the L-band Microwave Emission from the Biosphere (L-MEB) model in order to improve soil moisture retrievals from passive microwave observations. Data from the SMAP Validation Experiment 2012 conducted in Canada are used to develop and validate a simple model for the estimation of effective roughness parameters. Results show that the L-MEB roughness parameters can be empirically related to the observed brightness temperatures and the leaf area index of the vegetation. These results indicate that the roughness parameters are compensating for both roughness and vegetation effects. It is also shown, using a leave-one-out cross validation, that the model is able to accurately estimate the roughness parameters necessary for the inversion of the L-MEB model. In order to demonstrate the usefulness of the roughness parameterization, the performance of the model is compared to more traditional roughness formulations. Results indicate that the soil moisture retrieval error can be reduced to 0.054 m(3)/m(3) if the roughness formulation proposed in this study is implemented in the soil moisture retrieval algorithm. C1 [Martens, Brecht; Lievens, Hans; Verhoest, Niko E. C.] Univ Ghent, Lab Hydrol & Water Management, B-9000 Ghent, Belgium. [Colliander, Andreas] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. [Jackson, Thomas J.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Martens, B (reprint author), Univ Ghent, Lab Hydrol & Water Management, B-9000 Ghent, Belgium. EM Brecht.Martens@ugent.be RI Verhoest, Niko/C-9726-2010; OI Verhoest, Niko/0000-0003-4116-8881; Martens, Brecht/0000-0002-7368-7953 FU National Aeronautics and Space Administration FX The authors would like to thank the SMAPVEX12 investigators for making the data of the campaigns publicly available to the scientific community and the anonymous reviewers for their significant contribution to this paper. The second author is a Postdoctoral Research Fellow with the Research Foundation Flanders (FWO). Part of the work described in this paper was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. NR 49 TC 5 Z9 6 U1 4 U2 38 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 EI 1558-0644 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD JUL PY 2015 VL 53 IS 7 BP 4091 EP 4103 DI 10.1109/TGRS.2015.2390259 PG 13 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA CD9ZO UT WOS:000351461000042 ER PT J AU Ecker, G Zalapa, J Auer, C AF Ecker, Geoffrey Zalapa, Juan Auer, Carol TI Switchgrass (Panicum virgatum L.) Genotypes Differ between Coastal Sites and Inland Road Corridors in the Northeastern US SO PLOS ONE LA English DT Article ID ECOLOGICAL RISK-ASSESSMENT; PHRAGMITES-AUSTRALIS; LOWLAND SWITCHGRASS; COMMON REED; GENE FLOW; PLANTS; POPULATIONS; ADAPTATION; DIVERSITY; MARKERS AB Switchgrass (Panicum virgatum L.) is a North American grass that exhibits vast genetic diversity across its geographic range. In the Northeastern US, local switchgrass populations were restricted to a narrow coastal zone before European settlement, but current populations inhabit inland road verges raising questions about their origin and genetics. These questions are important because switchgrass lines with novel traits are being cultivated as a biofuel feedstock, and gene flow could impact the genetic integrity and distribution of local populations. This study was designed to determine if: 1) switchgrass plants collected in the Long Island Sound Coastal Lowland coastal Level IV ecoregion represented local populations, and 2) switchgrass plants collected from road verges in the adjacent inland regions were most closely related to local coastal populations or switchgrass from other geographic regions. The study used 18microsatellitemarkers to infer the genetic relationships between 122 collected switchgrass plants and a reference dataset consisting of 28 cultivars representing ecotypes, ploidy levels, and lineages from North America. Results showed that 84% of 88 plants collected in the coastal plants were most closely aligned with the Lowland tetraploid genetic pool. Among this group, 61 coastal plants were similar to, but distinct from, all Lowland tetraploid cultivars in the reference dataset leading to the designation of a genetic sub-population called the Southern New England Lowland Tetraploids. In contrast, 67% of 34 plants collected in road verges in the inland ecoregions were most similar to two Upland octoploid cultivars; only 24% of roadside plants were Lowland tetraploid. These results suggest that cryptic, non-local genotypes exist in road verges and that gene flow from biofuels plantations could contribute to further changes in switchgrass population genetics in the Northeast. C1 [Ecker, Geoffrey; Auer, Carol] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA. [Zalapa, Juan] Univ Wisconsin, USDA ARS, Dept Hort, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Ecker, G (reprint author), Univ Connecticut, Dept Plant Sci & Landscape Architecture, 1390 Storrs Rd,U 4163, Storrs, CT 06269 USA. EM Giecker@gmail.com FU Biotechnology Risk Assessment Grant from the United States Department of Agriculture, National Institute of Food and Agriculture [2008-39211-19566, 2011-33522-3081]; University of Connecticut FX The project was supported by Biotechnology Risk Assessment Grant award no. 2008-39211-19566 and no. 2011-33522-3081 from the United States Department of Agriculture, National Institute of Food and Agriculture to CA, (http://nifa.usda.gov/program/biotechnology-risk-assessment-research-gra nts-program). The University of Connecticut provided assistantship support to GE, (uconn.edu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 1 Z9 1 U1 2 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 30 PY 2015 VL 10 IS 6 AR e0130414 DI 10.1371/journal.pone.0130414 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1BR UT WOS:000358151300014 PM 26125564 ER PT J AU Sampson, JF Hasegawa, E Mulki, L Suryawanshi, A Jiang, SH Chen, WS Rabinovich, GA Connor, KM Panjwani, N AF Sampson, James F. Hasegawa, Eiichi Mulki, Lama Suryawanshi, Amol Jiang, Shuhong Chen, Wei-Sheng Rabinovich, Gabriel A. Connor, Kip M. Panjwani, Noorjahan TI Galectin-8 Ameliorates Murine Autoimmune Ocular Pathology and Promotes a Regulatory T Cell Response SO PLOS ONE LA English DT Article ID LEISHMANIA-MAJOR INFECTION; STEM-CELLS; IN-VIVO; DISEASE; UVEITIS; UVEORETINITIS; TH17; INFLAMMATION; SUPPRESSES; TOLERANCE AB Galectins have emerged as potent immunoregulatory agents that control chronic inflammation through distinct mechanisms. Here, we report that treatment with Galectin-8 (Gal-8), a tandem-repeat member of the galectin family, reduces retinal pathology and prevents photoreceptor cell damage in a murine model of experimental autoimmune uveitis. Gal-8 treatment increased the number of regulatory T cells (Treg) in both the draining lymph node (dLN) and the inflamed retina. Moreover, a greater percentage of Treg cells in the dLN and retina of Gal-8 treated animals expressed the inhibitory coreceptor cytotoxic T lymphocyte antigen (CTLA)-4, the immunosuppressive cytokine IL-10, and the tissue-homing integrin CD103. Treg cells in the retina of Gal-8-treated mice were primarily inducible Treg cells that lack the expression of neuropilin-1. In addition, Gal-8 treatment blunted production of inflammatory cytokines by retinal T helper type (T-H) 1 and T(H)17 cells. The effect of Gal-8 on T cell differentiation and/or function was specific for tissues undergoing an active immune response, as Gal-8 treatment had no effect on T cell populations in the spleen. Given the need for rational therapies for managing human uveitis, Gal-8 emerges as an attractive therapeutic candidate not only for treating retinal autoimmune diseases, but also for other T(H)1-and T(H)17-mediated inflammatory disorders. C1 [Sampson, James F.; Panjwani, Noorjahan] Tufts Univ, Sackler Sch Grad Biomed Sci, Program Immunol, Boston, MA 02111 USA. [Hasegawa, Eiichi; Mulki, Lama; Connor, Kip M.] Harvard Univ, Massachusetts Eye & Ear Infirm, Sch Med, Angiogenesis Lab,Dept Ophthalmol, Boston, MA 02114 USA. [Suryawanshi, Amol; Panjwani, Noorjahan] Tufts Univ, Sch Med, New England Eye Ctr, Dept Ophthalmol, Boston, MA 02111 USA. [Jiang, Shuhong] Tufts Univ, JM USDA HNRCA, Lab Nutr & Vis Res, Boston, MA 02111 USA. [Jiang, Shuhong; Panjwani, Noorjahan] Peoples Hosp Inner Mongolia, Dept Ophthalmol, Hohhot, Peoples R China. [Chen, Wei-Sheng] Tufts Univ, Sackler Sch Grad Biomed Sci, Cell Mol & Dev Biol, Boston, MA 02111 USA. [Rabinovich, Gabriel A.] Consejo Nacl Invest Cient & Tecn, Lab Inmunopatol, IBYME, RA-1033 Buenos Aires, DF, Argentina. RP Connor, KM (reprint author), Harvard Univ, Massachusetts Eye & Ear Infirm, Sch Med, Angiogenesis Lab,Dept Ophthalmol, Boston, MA 02114 USA. EM kip_connor@meei.harvard.edu; Noorjahan.panjwani@tufts.edu OI Connor, Kip/0000-0002-2048-9080 FU National Eye Institute [R01EY007088, R01EY009349, R01EY022084-01/S1]; Mass Lions Eye Research fund; New England Corneal Transplant Fund; Research to Prevent Blindness FX This work was generously supported by National Eye Institute (https://www.nei.nih.gov/) Grants R01EY007088 (NP), R01EY009349 (NP), R01EY022084-01/S1 (KMC), Mass Lions Eye Research fund (NP), New England Corneal Transplant Fund (NP) and an unrestricted award from Research to Prevent Blindness (http://www.rpbusa.org/rpb/NP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 3 Z9 3 U1 0 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 30 PY 2015 VL 10 IS 6 AR e0130772 DI 10.1371/journal.pone.0130772 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1BR UT WOS:000358151300025 PM 26126176 ER PT J AU Barbero, R Abatzoglou, JT Kolden, CA Hegewisch, KC Larkin, NK Podschwit, H AF Barbero, Renaud Abatzoglou, John T. Kolden, Crystal A. Hegewisch, Katherine C. Larkin, Narasimhan K. Podschwit, Harry TI Multi-scalar influence of weather and climate on very large-fires in the Eastern United States SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article DE very large-fires; Eastern United States; temporal scales; climate-fire linkages ID WILDFIRE ACTIVITY; BURNED AREA; REGIMES AB A majority of area burned in the Eastern United States (EUS) results from a limited number of exceptionally large wildfires. Relationships between climatic conditions and the occurrence of very large-fires (VLF) in the EUS were examined using composite and climate-niche analyses that consider atmospheric factors across inter-annual, sub-seasonal and synoptic temporal scales. While most large-fires in the EUS coincided with below normal fuel moisture and elevated fire weather, VLF preferentially occurred during a long-term drought accompanied by more acute sub-seasonal drought realized through fuel moisture stress and elevated fire-weather conditions. These results were corroborated across the EUS, with varying influences of drought, fire danger and fire weather discriminating VLF from other large fires across different geographical regions. We also show that the probability of VLF conditioned by fire occurrence increases when long-term drought, depleted fuel moisture and elevated fire weather align. This framework illustrates the compounding role of different timescales in VLF occurrence and serves as a basis for improving VLF predictions with seasonal climate forecasts and climate change scenarios. C1 [Barbero, Renaud; Abatzoglou, John T.; Kolden, Crystal A.; Hegewisch, Katherine C.] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. [Larkin, Narasimhan K.; Podschwit, Harry] US Forest Serv, Pacific Wildland Fire Sci Lab, Seattle, WA USA. RP Barbero, R (reprint author), Univ Idaho, Dept Geog, 875 Perimeter Dr MS 3021, Moscow, ID 83844 USA. EM renaudb@uidaho.edu OI Abatzoglou, John/0000-0001-7599-9750 FU Joint Fire Science Program [11-1-7-4] FX We acknowledge the helpful feedback from three anonymous reviewers. This research was supported by the Joint Fire Science Program award number 11-1-7-4. NR 35 TC 8 Z9 8 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 EI 1097-0088 J9 INT J CLIMATOL JI Int. J. Climatol. PD JUN 30 PY 2015 VL 35 IS 8 BP 2180 EP 2186 DI 10.1002/joc.4090 PG 7 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CM5OG UT WOS:000357737000037 ER PT J AU Comoy, EE Mikol, J Luccantoni-Freire, S Correia, E Lescoutra-Etchegaray, N Durand, V Dehen, C Andreoletti, O Casalone, C Richt, JA Greenlee, JJ Baron, T Benestad, SL Brown, P Deslys, JP AF Comoy, Emmanuel E. Mikol, Jacqueline Luccantoni-Freire, Sophie Correia, Evelyne Lescoutra-Etchegaray, Nathalie Durand, Valerie Dehen, Capucine Andreoletti, Olivier Casalone, Cristina Richt, Juergen A. Greenlee, Justin J. Baron, Thierry Benestad, Sylvie L. Brown, Paul Deslys, Jean-Philippe TI Transmission of scrapie prions to primate after an extended silent incubation period SO SCIENTIFIC REPORTS LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; BOVINE SPONGIFORM ENCEPHALOPATHY; CHRONIC WASTING DISEASE; CYNOMOLGUS MACAQUES; NONHUMAN-PRIMATES; BSE TRANSMISSION; NATURAL SCRAPIE; AGENT; PROTEIN; SHEEP AB Classical bovine spongiform encephalopathy (c-BSE) is the only animal prion disease reputed to be zoonotic, causing variant Creutzfeldt-Jakob disease (vCJD) in humans and having guided protective measures for animal and human health against animal prion diseases. Recently, partial transmissions to humanized mice showed that the zoonotic potential of scrapie might be similar to c-BSE. We here report the direct transmission of a natural classical scrapie isolate to cynomolgus macaque, a highly relevant model for human prion diseases, after a 10-year silent incubation period, with features similar to those reported for human cases of sporadic CJD. Scrapie is thus actually transmissible to primates with incubation periods compatible with their life expectancy, although fourfold longer than BSE. Long-term experimental transmission studies are necessary to better assess the zoonotic potential of other prion diseases with high prevalence, notably Chronic Wasting Disease of deer and elk and atypical/Nor98 scrapie. C1 [Comoy, Emmanuel E.; Mikol, Jacqueline; Luccantoni-Freire, Sophie; Correia, Evelyne; Lescoutra-Etchegaray, Nathalie; Durand, Valerie; Dehen, Capucine; Brown, Paul; Deslys, Jean-Philippe] CEA, Atom Energy Commiss, Inst Emerging Dis & Innovat Therapies iMETI, Div Prions & Related Dis SEPIA, F-92265 Fontenay Aux Roses, France. [Andreoletti, Olivier] INRA, ENVT, F-31076 Toulouse, France. [Casalone, Cristina] Ist Zooprofilattico Sperimentale Piemonte, I-10154 Turin, Italy. [Richt, Juergen A.; Greenlee, Justin J.] Natl Anim Dis, USDA ARS, Virus & Prion Res Unit, Ames, IA 50010 USA. [Baron, Thierry] ANSES, F-69364 Lyon, France. [Benestad, Sylvie L.] Norwegian Vet Inst, N-0106 Oslo, Norway. RP Comoy, EE (reprint author), CEA, Atom Energy Commiss, Inst Emerging Dis & Innovat Therapies iMETI, Div Prions & Related Dis SEPIA, 18 Route Panorama,BP6, F-92265 Fontenay Aux Roses, France. EM emmanuel.comoy@cea.fr NR 56 TC 8 Z9 9 U1 3 U2 18 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 30 PY 2015 VL 5 AR 11573 DI 10.1038/srep11573 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL6UU UT WOS:000357103800001 PM 26123044 ER PT J AU Dubey, JP Houk, AE Verma, SK Calero-Bernal, R Humphreys, JG Lindsay, DS AF Dubey, J. P. Houk, A. E. Verma, S. K. Calero-Bernal, R. Humphreys, J. G. Lindsay, D. S. TI Experimental transmission of Cystoisospora felis-like coccidium from bobcat (Lynx rufus) to the domestic cat (Felis catus) SO VETERINARY PARASITOLOGY LA English DT Article DE Cystoisospora felis; Bobcat (Lynx rufus); Domestic cat (Felis catus); Transmission via mice ID TOXOPLASMA-GONDII OOCYSTS; ISOSPORA-FELIS; LIFE-CYCLE; IMMUNITY; RIVOLTA; MICE AB Cystoisospora felis is an ubiquitous coccidian of cats. The domestic cat (Fells catus) is its definitive host and several mammalian and avian species are its optional intermediate/transport hosts. Nothing is known if it is transmissible to wild felids. In the present study C.felis-like oocysts were found in two naturally infected bobcats (Lynx rufus) from Pennsylvania. To study transmission of C felis-like parasite from bobcats to domestic cats, sporulated oocysts of C fells-like from one bobcat were orally inoculated into interferon gamma gene knockout (KO) mice, and 56 days later tissues of KO mice were fed to two coccidian-free cats; two littermate cats were uninoculated controls. The inoculated cats and controls were euthanized five and seven days later, and their small intestines were studied histologically. One inoculated cat excreted C. felis-like oocysts seven days post inoculation (p.i.) and was immediately euthanized. Mature schizonts, mature male and female gamonts, and unsporulated oocysts were found in the lamina propria of small intestine; these stages were morphologically similar to C. felis of domestic cats. No parasites were seen in histological sections of small intestines of the remaining three cats. The experiment was terminated at seven days p.i. (minimum prepatent period for C. fells) to minimize spread of this highly infectious parasite to other cats. Although oocysts of the parasite in bobcats were morphologically similar to C. felis of domestic cats, the endogenous stages differed in their location of development. The bobcat derived parasite was located in the lamina propria of ileum whereas all endogenous stages of C. felis of domestic cats are always located in enterocytes of intestinal epithelium. Characterization of DNA isolated from C. fells-like oocysts from the donor bobcat revealed that sequences of the ITS1 region was only 87% similar to the ITS1 region of C. felis from domestic cats. These results indicate that the parasite in bobcat is likely different than C. felis of cats. Published by Elsevier B.V. C1 [Dubey, J. P.; Verma, S. K.; Calero-Bernal, R.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Houk, A. E.; Lindsay, D. S.] Virginia Tech, Dept Biomed Sci & Pathol, Virginia Maryland Reg Coll Vet Med, Blacksburg, VA 24061 USA. [Humphreys, J. G.] Indiana Univ Penn, Dept Biol, Indiana, PA 15705 USA. RP Dubey, JP (reprint author), ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov RI Lindsay, David/G-8891-2016 OI Lindsay, David/0000-0002-0592-8321 NR 14 TC 0 Z9 0 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUN 30 PY 2015 VL 211 IS 1-2 BP 35 EP 39 DI 10.1016/j.vetpar.2015.03.027 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CL5EH UT WOS:000356982100006 PM 25964236 ER PT J AU Bowles, TM Hollander, AD Steenwerth, K Jackson, LE AF Bowles, Timothy M. Hollander, Allan D. Steenwerth, Kerri Jackson, Louise E. TI Tightly-Coupled Plant-Soil Nitrogen Cycling: Comparison of Organic Farms across an Agricultural Landscape SO PLOS ONE LA English DT Article ID CONVENTIONAL CROPPING SYSTEMS; ECOSYSTEM SERVICES; PROCESSING TOMATO; SMALLHOLDER FARMS; N FERTILIZER; PCR DATA; NITRATE; MANAGEMENT; AVAILABILITY; AMMONIUM AB How farming systems supply sufficient nitrogen (N) for high yields but with reduced N losses is a central challenge for reducing the tradeoffs often associated with N cycling in agriculture. Variability in soil organic matter and management of organic farms across an agricultural landscape may yield insights for improving N cycling and for evaluating novel indicators of N availability. We assessed yields, plant-soil N cycling, and root expression of N metabolism genes across a representative set of organic fields growing Roma-type tomatoes (Solanum lycopersicum L.) in an intensively-managed agricultural landscape in California, USA. The fields spanned a three-fold range of soil carbon (C) and N but had similar soil types, texture, and pH. Organic tomato yields ranged from 22.9 to 120.1 Mg ha(-1) with a mean similar to the county average (86.1 Mg ha(-1)), which included mostly conventionally-grown tomatoes. Substantial variability in soil inorganic N concentrations, tomato N, and root gene expression indicated a range of possible tradeoffs between yields and potential for N losses across the fields. Fields showing evidence of tightly-coupled plant-soil N cycling, a desirable scenario in which high crop yields are supported by adequate N availability but low potential for N loss, had the highest total and labile soil C and N and received organic matter inputs with a range of N availability. In these fields, elevated expression of a key gene involved in root N assimilation, cytosolic glutamine synthetase GS1, confirmed that plant N assimilation was high even when inorganic N pools were low. Thus tightlycoupled N cycling occurred on several working organic farms. Novel combinations of N cycling indicators (i.e. inorganic N along with soil microbial activity and root gene expression for N assimilation) would support adaptive management for improved N cycling on organic as well as conventional farms, especially when plant-soil N cycling is rapid. C1 [Bowles, Timothy M.; Jackson, Louise E.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Hollander, Allan D.] Univ Calif Davis, Dept Environm Sci & Policy, Environm Informat Ctr, Davis, CA 95616 USA. [Steenwerth, Kerri] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA USA. RP Bowles, TM (reprint author), Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. EM timothy.bowles@gmail.com FU United States Department of Agriculture (USDA) Organic Agriculture Research and Extension Initiative (OREI) award [2009-01415]; National Science Foundation (NSF) FX The funding came from a United States Department of Agriculture (USDA) Organic Agriculture Research and Extension Initiative (OREI) award number 2009-01415 to LEJ; http://www.nifa.usda.gov/funding/rfas/OREI.html. Additional funding came from a National Science Foundation (NSF) Graduate Research Fellowship to TMB; https://www.nsfgrfp.org/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 3 Z9 3 U1 14 U2 47 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 29 PY 2015 VL 10 IS 6 AR e0131888 DI 10.1371/journal.pone.0131888 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1BL UT WOS:000358150400173 PM 26121264 ER PT J AU Groenendyk, DG Ferre, TPA Thorp, KR Rice, AK AF Groenendyk, Derek G. Ferre, Ty P. A. Thorp, Kelly R. Rice, Amy K. TI Hydrologic-Process-Based Soil Texture Classifications for Improved Visualization of Landscape Function SO PLOS ONE LA English DT Article ID CLUSTER-ANALYSIS; HYDRAULIC CONDUCTIVITY; TAXONOMY; MODELS AB Soils lie at the interface between the atmosphere and the subsurface and are a key component that control ecosystem services, food production, and many other processes at the Earth's surface. There is a long-established convention for identifying and mapping soils by texture. These readily available, georeferenced soil maps and databases are used widely in environmental sciences. Here, we show that these traditional soil classifications can be inappropriate, contributing to bias and uncertainty in applications from slope stability to water resource management. We suggest a new approach to soil classification, with a detailed example from the science of hydrology. Hydrologic simulations based on common meteorological conditions were performed using HYDRUS-1D, spanning textures identified by the United States Department of Agriculture soil texture triangle. We consider these common conditions to be: drainage from saturation, infiltration onto a drained soil, and combined infiltration and drainage events. Using a k-means clustering algorithm, we created soil classifications based on the modeled hydrologic responses of these soils. The hydrologic-process-based classifications were compared to those based on soil texture and a single hydraulic property, K-s. Differences in classifications based on hydrologic response versus soil texture demonstrate that traditional soil texture classification is a poor predictor of hydrologic response. We then developed a QGIS plugin to construct soil maps combining a classification with georeferenced soil data from the Natural Resource Conservation Service. The spatial patterns of hydrologic response were more immediately informative, much simpler, and less ambiguous, for use in applications ranging from trafficability to irrigation management to flood control. The ease with which hydrologic-process-based classifications can be made, along with the improved quantitative predictions of soil responses and visualization of landscape function, suggest that hydrologic-process-based classifications should be incorporated into environmental process models and can be used to define application-specific maps of hydrologic function. C1 [Groenendyk, Derek G.; Ferre, Ty P. A.] Univ Arizona, Hydrol & Water Resources, Tucson, AZ 85721 USA. [Thorp, Kelly R.] USDA ARS, United States Arid Land Agr Res Ctr, Maricopa, AZ USA. [Rice, Amy K.] Colorado Sch Mines, Hydrol Sci & Engn Program, Golden, CO 80401 USA. RP Groenendyk, DG (reprint author), Univ Arizona, Hydrol & Water Resources, Tucson, AZ 85721 USA. EM drockg@email.arizona.edu RI Thorp, Kelly/C-2013-2009 OI Thorp, Kelly/0000-0001-9168-875X FU Phoenix Chapter of Achievement Rewards for College Scientists Foundation (ARCS), Inc.; National Aeronautics and Space Administration (NASA) Research Opportunities in Space and Earth Sciences (ROSES) Terrestrial Ecology Program [NNH09ZDA001N] FX Funding was provided by the Phoenix Chapter of the Achievement Rewards for College Scientists Foundation (ARCS), Inc., www.arcsfoundation.org, (DGG) and through the National Aeronautics and Space Administration (NASA) Research Opportunities in Space and Earth Sciences (ROSES) Terrestrial Ecology Program, Award #NNH09ZDA001N, http://cce.nasa.gov/terrestrial_ecology/index.html (DGG, KRT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 0 Z9 0 U1 5 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 29 PY 2015 VL 10 IS 6 AR e0131299 DI 10.1371/journal.pone.0131299 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1BL UT WOS:000358150400094 PM 26121466 ER PT J AU Steinmann, N Corona, M Neumann, P Dainat, B AF Steinmann, Nadja Corona, Miguel Neumann, Peter Dainat, Benjamin TI Overwintering Is Associated with Reduced Expression of Immune Genes and Higher Susceptibility to Virus Infection in Honey Bees SO PLOS ONE LA English DT Article ID DEFORMED WING VIRUS; DIVISION-OF-LABOR; APIS-MELLIFERA; JUVENILE-HORMONE; VARROA-DESTRUCTOR; WINTER HONEYBEES; COLONY LOSSES; LIFE-SPAN; VITELLOGENIN; PROPHENOLOXIDASE AB The eusocial honey bee, Apis mellifera, has evolved remarkable abilities to survive extreme seasonal differences in temperature and availability of resources by dividing the worker caste into two groups that differ in physiology and lifespan: summer and winter bees. Most of the recent major losses of managed honey bee colonies occur during the winter, suggesting that winter bees may have compromised immune function and higher susceptibility to diseases. We tested this hypothesis by comparing the expression of eight immune genes and naturally occurring infection levels of deformed wing virus (DWV), one of the most widespread viruses in A. mellifera populations, between summer and winter bees. Possible interactions between immune response and physiological activity were tested by measuring the expression of vitellogenin and methyl farnesoate epoxidase, a gene coding for the last enzyme involved in juvenile hormone biosynthesis. Our data show that high DWV loads in winter bees correlate with reduced expression of genes involved in the cellular immune response and physiological activity and high expression of humoral immune genes involved in antibacterial defense compared with summer bees. This expression pattern could reflect evolutionary adaptations to resist bacterial pathogens and economize energy during the winter under a pathogen landscape with reduced risk of pathogenic viral infections. The outbreak of Varroa destructor infestation could have overcome these adaptations by promoting the transmission of viruses. Our results suggest that reduced cellular immune function during the winter may have increased honey bee's susceptibility to DWV. These results contribute to our understanding of honey bee colony losses in temperate regions. C1 [Steinmann, Nadja; Dainat, Benjamin] Agroscope Swiss Bee Res Ctr Liebefeld, CH-3003 Bern, Switzerland. [Steinmann, Nadja; Neumann, Peter] Univ Bern, Vetsuisse Fac, Inst Bee Hlth, CH-3001 Bern, Switzerland. [Steinmann, Nadja; Neumann, Peter] Univ Bern, Fac Med, CH-3001 Bern, Switzerland. [Corona, Miguel] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Dainat, Benjamin] Swiss Bee Hlth Serv, CH-3003 Bern, Switzerland. RP Dainat, B (reprint author), Agroscope Swiss Bee Res Ctr Liebefeld, Schwarzenburgstr 161, CH-3003 Bern, Switzerland. EM benjamin.dainat@gmail.com FU Swiss Federal Veterinary Office [1.11.02] FX This study was partially funded by the Swiss Federal Veterinary Office grant 1.11.02. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This study did not receive any further additional funding. NR 69 TC 5 Z9 6 U1 5 U2 47 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 29 PY 2015 VL 10 IS 6 AR e0129956 DI 10.1371/journal.pone.0129956 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1BL UT WOS:000358150400030 PM 26121358 ER PT J AU Seo, JH Chang, TH Lee, J Sabo, R Zhou, WD Cai, ZY Gong, SQ Ma, ZQ AF Seo, Jung-Hun Chang, Tzu-Hsuan Lee, Jaeseong Sabo, Ronald Zhou, Weidong Cai, Zhiyong Gong, Shaoqin Ma, Zhenqiang TI Microwave flexible transistors on cellulose nanofibrillated fiber substrates SO APPLIED PHYSICS LETTERS LA English DT Article ID THIN-FILM TRANSISTORS; SILICON NANOMEMBRANES; SOLAR-CELLS; TRANSPARENT; PAPER; ELECTRONICS AB In this paper, we demonstrate microwave flexible thin-film transistors (TFTs) on biodegradable substrates towards potential green portable devices. The combination of cellulose nanofibrillated fiber (CNF) substrate, which is a biobased and biodegradable platform, with transferrable single crystalline Si nanomembrane (Si NM), enables the realization of truly biodegradable, flexible, and high performance devices. Double-gate flexible Si NM TFTs built on a CNF substrate have shown an electron mobility of 160 cm(2)/V.s and f(T) and f(max) of 4.9GHz and 10.6 GHz, respectively. This demonstration proves the microwave frequency capability and, considering today's wide spread use of wireless devices, thus indicates the much wider utility of CNF substrates than that has been demonstrated before. The demonstration may also pave the way toward portable green devices that would generate less persistent waste and save more valuable resources. (C) 2015 AIP Publishing LLC. C1 [Seo, Jung-Hun; Chang, Tzu-Hsuan; Lee, Jaeseong; Ma, Zhenqiang] Univ Wisconsin, Dept Elect & Comp Engn, Madison, WI 53706 USA. [Sabo, Ronald; Cai, Zhiyong] USDA, Forest Prod Lab, Madison, WI 53726 USA. [Zhou, Weidong] Univ Texas Arlington, Dept Elect Engn, Arlington, TX 76019 USA. [Gong, Shaoqin] Univ Wisconsin, Wisconsin Inst Discovery, Dept Biomed Engn, Madison, WI 53706 USA. [Gong, Shaoqin] Univ Wisconsin, Mat Sci Program, Madison, WI 53706 USA. RP Gong, SQ (reprint author), Univ Wisconsin, Wisconsin Inst Discovery, Dept Biomed Engn, Madison, WI 53706 USA. EM sgong@engr.wisc.edu; mazq@engr.wisc.edu RI Seo, Jung-Hun/G-2659-2012; OI Seo, Jung-Hun/0000-0002-5039-2503; Lee, Jaeseong/0000-0001-6549-1021 FU PECASE award [FA9550-09-1-0482] FX This work was supported by a PECASE award under Grant No. FA9550-09-1-0482. The program manager at AFOSR is Dr. Gernot Pomrenke. NR 29 TC 13 Z9 13 U1 3 U2 27 PU AMER INST PHYSICS PI MELVILLE PA 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA SN 0003-6951 EI 1077-3118 J9 APPL PHYS LETT JI Appl. Phys. Lett. PD JUN 29 PY 2015 VL 106 IS 26 AR 262101 DI 10.1063/1.4921077 PG 4 WC Physics, Applied SC Physics GA CM4YQ UT WOS:000357693200020 ER PT J AU O'Neil, CE Iii, VLF Nicklas, TA AF O'Neil, Carol E. Iii, Victor L. Fulgoni Nicklas, Theresa A. TI Tree Nut consumption is associated with better adiposity measures and cardiovascular and metabolic syndrome health risk factors in US Adults: NHANES 2005-2010 SO NUTRITION JOURNAL LA English DT Article DE Tree Nuts; NHANES; Adults; Metabolic Syndrome; Cardiovascular Risk Factors ID RANDOMIZED CROSSOVER TRIAL; ENDOTHELIAL FUNCTION; WALNUT CONSUMPTION; HYPERCHOLESTEROLEMIC SUBJECTS; INFLAMMATORY MARKERS; INSULIN-RESISTANCE; HDL-CHOLESTEROL; NUTRIENT INTAKE; BLOOD-PRESSURE; LIPID-LEVELS AB Introduction: Previous research has shown inconsistencies in the association of tree nut consumption with risk factors for cardiovascular disease (CVD) and metabolic syndrome (MetS). Objective: To determine the association of tree nut consumption with risk factors for CVD and for MetS in adults. Methods: NHANES 2005-2010 data were used to examine the associations of tree nut consumption with health risks in adults 19+ years (n = 14,386; 51 % males). Tree nuts were: almonds, Brazil nuts, cashews, filberts [hazelnuts], macadamias, pecans, pine nuts, pistachios, and walnuts. Group definitions were non-consumers < 1/4 ounce/day and consumers of >= 1/4 ounce/day tree nuts using data from 24-h dietary recalls. Means and ANOVA (covariate adjusted) were determined using appropriate sample weights. Using logistic regression, odds ratios of being overweight (OW)/obese (OB) (body mass index [BMI] > 25/< 30 and >= 30, respectively) and having CVRF or MetS, were determined. Results: Tree nut consumption was associated with lower BMI (p = 0.004), waist circumference (WC) (p = 0.008), systolic blood pressure (BP) (p = 0.001), Homeostatic Model Assessment-Insulin Resistance (p = 0.043), and higher high density lipoprotein-cholesterol (p = 0.022), compared with no consumption, and a lower likelihood of OB (-25 %), OW/OB (-23 %), and elevated WC (-21 %). Conclusions: Tree nut consumption was associated with better weight status and some CVRF and MetS components. C1 [O'Neil, Carol E.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Iii, Victor L. Fulgoni] Nutr Impact LLC, Battle Creek, MI 49014 USA. [Nicklas, Theresa A.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP O'Neil, CE (reprint author), Louisiana State Univ, Ctr Agr, 261 Knapp Hall,110 LSU Union Sq, Baton Rouge, LA 70803 USA. EM coneil1@lsu.edu FU International Tree Nut Council Nutrition Research & Education Foundation; USDA-Agricultural Research Service [58-3092-5-001]; USDA Hatch Project [LAB 94209] FX With the exception of the acknowledged funding sources, the authors declare no conflicts of interest related to this manuscript. This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. Support was obtained from the International Tree Nut Council Nutrition Research & Education Foundation and USDA-Agricultural Research Service through specific cooperative agreement 58-3092-5-001. Partial support was received from the USDA Hatch Project LAB 94209. The funding sources had no input into the study design or the interpretation of the results. NR 54 TC 4 Z9 4 U1 13 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1475-2891 J9 NUTR J JI Nutr. J. PD JUN 28 PY 2015 VL 14 AR 64 DI 10.1186/s12937-015-0052-x PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CL5NM UT WOS:000357006600001 PM 26123047 ER PT J AU Tuttle, JR Nah, G Duke, MV Alexander, DC Guan, XY Song, QX Chen, ZJ Scheffler, BE Haigler, CH AF Tuttle, John R. Nah, Gyoungju Duke, Mary V. Alexander, Danny C. Guan, Xueying Song, Qingxin Chen, Z. Jeffrey Scheffler, Brian E. Haigler, Candace H. TI Metabolomic and transcriptomic insights into how cotton fiber transitions to secondary wall synthesis, represses lignification, and prolongs elongation SO BMC GENOMICS LA English DT Article DE Ascorbate; Cell elongation; Cell wall synthesis; Cotton fiber development; Gossypium; Lignification; Reactive oxygen species; Metabolomics; RNA Seq transcriptomics ID GOSSYPIUM-HIRSUTUM L.; PROGRAMMED CELL-DEATH; ROOT-HAIR-CELLS; GENE-EXPRESSION; DESICCATION TOLERANCE; ARABIDOPSIS-THALIANA; LIGNIN BIOSYNTHESIS; FUNCTIONAL GENOMICS; CELLULOSE SYNTHESIS; HYDROGEN-PEROXIDE AB Background: The morphogenesis of single-celled cotton fiber includes extreme elongation and staged cell wall differentiation. Designing strategies for improving cotton fiber for textiles and other uses relies on uncovering the related regulatory mechanisms. In this research we compared the transcriptomes and metabolomes of two Gossypium genotypes, Gossypium barbadense cv Phytogen 800 and G. hirsutum cv Deltapine 90. When grown in parallel, the two types of fiber developed similarly except for prolonged fiber elongation in the G. barbadense cultivar. The data were collected from isolated fibers between 10 to 28 days post anthesis (DPA) representing: primary wall synthesis to support elongation; transitional cell wall remodeling; and secondary wall cellulose synthesis, which was accompanied by continuing elongation only in G. barbadense fiber. Results: Of 206 identified fiber metabolites, 205 were held in common between the two genotypes. Approximately 38,000 transcripts were expressed in the fiber of each genotype, and these were mapped to the reference set and interpreted by homology to known genes. The developmental changes in the transcriptomes and the metabolomes were compared within and across genotypes with several novel implications. Transitional cell wall remodeling is a distinct stable developmental stage lasting at least four days (18 to 21 DPA). Expression of selected cell wall related transcripts was similar between genotypes, but cellulose synthase gene expression patterns were more complex than expected. Lignification was transcriptionally repressed in both genotypes. Oxidative stress was lower in the fiber of G. barbadense cv Phytogen 800 as compared to G. hirsutum cv Deltapine 90. Correspondingly, the G. barbadense cultivar had enhanced capacity for management of reactive oxygen species during its prolonged elongation period, as indicated by a 138-fold increase in ascorbate concentration at 28 DPA. Conclusions: The parallel data on deep-sequencing transcriptomics and non-targeted metabolomics for two genotypes of single-celled cotton fiber showed that a discrete developmental stage of transitional cell wall remodeling occurs before secondary wall cellulose synthesis begins. The data showed how lignification can be transcriptionally repressed during secondary cell wall synthesis, and they implicated enhanced capacity to manage reactive oxygen species through the ascorbate-glutathione cycle as a positive contributor to fiber length. C1 [Tuttle, John R.; Haigler, Candace H.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Nah, Gyoungju; Guan, Xueying; Song, Qingxin; Chen, Z. Jeffrey] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA. [Nah, Gyoungju; Guan, Xueying; Song, Qingxin; Chen, Z. Jeffrey] Univ Texas Austin, Ctr Computat Biol & Bioinformat, Austin, TX 78712 USA. [Duke, Mary V.; Scheffler, Brian E.] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Alexander, Danny C.] Metabolon Inc, Durham, NC 27713 USA. [Haigler, Candace H.] N Carolina State Univ, Dept Plant & Microbial Biol, Raleigh, NC 27695 USA. RP Haigler, CH (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM candace_haigler@ncsu.edu OI Scheffler, Brian/0000-0003-1968-8952 FU National Science Foundation grant [1025947] FX We thank: Cory Dashiell for producing the sequencing libraries; Dr. Joshua Udall for contributing A genome ESTs used in our RNAseq mapping reference; and Dr. Michael R. Stiff for helpful discussions. This work was supported by a National Science Foundation grant to ZJC, BES, and CHH (award # 1025947). The funding agency had no role in the collection, analysis, and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. NR 115 TC 5 Z9 5 U1 5 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 27 PY 2015 VL 16 AR 477 DI 10.1186/s12864-015-1708-9 PG 28 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CL4HV UT WOS:000356914100001 PM 26116072 ER PT J AU Obermayr, U Ruther, J Bernier, UR Rose, A Geier, M AF Obermayr, Ulla Ruther, Joachim Bernier, Ulrich R. Rose, Andreas Geier, Martin TI Y Evaluation of a Push-Pull Approach for Aedes aegypti (L.) Using a Novel Dispensing System for Spatial Repellents in the Laboratory and in a Semi-Field Environment SO PLOS ONE LA English DT Article ID L-LACTIC ACID; CONTROL STRATEGY; FIELD EFFICACY; ESSENTIAL OIL; BG-SENTINEL; CATNIP-OIL; MOSQUITO; CULICIDAE; DIPTERA; TRAPS AB The increase in insecticide resistant mosquito populations necessitates the exploration of novel vector control intervention measures. Push-pull strategies for insect control have been successful when used in integrated crop pest management. Through the combinatory use of deterring and attracting stimuli, the abundance of insect pests can be changed in a given area. A push-pull strategy might also significantly reduce human-vector contacts and augment existing mosquito control strategies, e.g. through the combination of an attractive trapping system and a potent spatial repellent. Our approach includes the BG-Sentinel (BGS) trap in combination with catnip oil (Nepeta cataria), a known spatial repellent for Aedes aegypti. To impart a deterrent effect on mosquitoes at a distance, a homogenous and continuous dispersal of volatile repellent compounds is crucial. We have developed a repellent dispensing system that is easy to use and provides a homogenous dispersal of repellent in an air curtain. The use of five 9 V fans and custom-made repellent sachets containing 10% catnip essential oil created a repellent loaded air curtain that provided coverage of an area of 2 m(2) (1.2 x 1.65 m). Air was sampled at four different heights in the curtain and analysed via thermal desorption (TD) and consecutive gas chromatography-mass spectrometry (GC-MS). Nepetalactone, the main constituent of the oil, was detected in air at a concentration range of 80 to 100 mu g/m(3) and the amounts were comparable at all four sampling positions. When a human volunteer was sitting behind the repellent curtain and a BGS trap was installed in front of the curtain in laboratory push-pull trials, Ae. aegypti landing collections decreased significantly by 50% compared to repellent-free controls. However, in a semi-field environment, comparable protective effects could not be achieved and further research on suitable repellent concentrations for outdoor implementation will be required. C1 [Obermayr, Ulla; Rose, Andreas; Geier, Martin] Biogents AG, D-93055 Regensburg, Germany. [Ruther, Joachim] Univ Regensburg, Inst Zool, D-93053 Regensburg, Germany. [Bernier, Ulrich R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Obermayr, U (reprint author), Biogents AG, Weissenburgstr 22, D-93055 Regensburg, Germany. EM ulla.obermayr@biogents.com FU Biogents AG, Regensburg, Germany FX The project was fully funded by Biogents AG, Regensburg, Germany. Funding was received by UO. The funder provided support in the form of salaries for author UO, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the 'author contributions' section. NR 49 TC 2 Z9 2 U1 3 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 26 PY 2015 VL 10 IS 6 AR e0129878 DI 10.1371/journal.pone.0129878 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1AM UT WOS:000358147500041 PM 26115365 ER PT J AU Girouard, N Schueneman, GT Shofner, ML Meredith, JC AF Girouard, Natalie Schueneman, Gregory T. Shofner, Meisha L. Meredith, J. Carson TI Exploiting colloidal interfaces to increase dispersion, performance, and pot-life in cellulose nanocrystal/waterborne epoxy composites SO POLYMER LA English DT Article DE Interfaces; Colloidal stability; Polymer nanocomposite; Cellulose ID PICKERING EMULSIONS; SILICA SURFACES; OCTADECYLTRICHLOROSILANE; NANOCOMPOSITES; NANOCRYSTALS; WHISKERS; BIOCOMPOSITES; ADSORPTION; KINETICS; BEHAVIOR AB In this study, cellulose nanocrystals (CNCs) are incorporated into a waterborne epoxy resin following two processing protocols that vary by order of addition. The processing protocols produce different levels of CNC dispersion in the resulting composites. The more homogeneously dispersed composite has a higher storage modulus and work of fracture at temperatures less than the glass transition temperature. Some properties related to the component interactions, such as thermal degradation and moisture content, are similar for both composite systems. The mechanism of dispersion is probed with electrophoretic measurements and electron microscopy, and based on these results, it is hypothesized that CNC preaddition facilitates the formation of a CNC-coated epoxy droplet, promoting CNC dispersion and giving the epoxide droplets added electrostatic stability. The implication of these structural changes in polymer network formation results in an extension of the epoxy and crosslinker mixture's pot life by three orders of magnitude. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Girouard, Natalie; Meredith, J. Carson] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA. [Schueneman, Gregory T.] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Shofner, Meisha L.] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. [Shofner, Meisha L.; Meredith, J. Carson] Georgia Inst Technol, Renewable Bioprod Inst, Atlanta, GA 30332 USA. RP Meredith, JC (reprint author), Georgia Inst Technol, Sch Chem & Biomol Engn, 311 Ferst Dr, Atlanta, GA 30332 USA. EM carson.meredith@chbe.gatech.edu RI Meredith, Carson/B-3323-2009 OI Meredith, Carson/0000-0003-2519-5003 FU United States Department of Agriculture Forest Products Laboratory FX Funding for this research was provided by the United States Department of Agriculture Forest Products Laboratory. NR 38 TC 8 Z9 8 U1 10 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0032-3861 EI 1873-2291 J9 POLYMER JI Polymer PD JUN 26 PY 2015 VL 68 BP 111 EP 121 DI 10.1016/j.polymer.2015.05.009 PG 11 WC Polymer Science SC Polymer Science GA CM5BN UT WOS:000357701900014 ER PT J AU Nallar, R Papp, Z Epp, T Leighton, FA Swafford, SR DeLiberto, TJ Dusek, RJ Ip, HS Hall, J Berhane, Y Gibbs, SEJ Soos, C AF Nallar, Rodolfo Papp, Zsuzsanna Epp, Tasha Leighton, Frederick A. Swafford, Seth R. DeLiberto, Thomas J. Dusek, Robert J. Ip, Hon S. Hall, Jeffrey Berhane, Yohannes Gibbs, Samantha E. J. Soos, Catherine TI Demographic and Spatiotemporal Patterns of Avian Influenza Infection at the Continental Scale, and in Relation to Annual Life Cycle of a Migratory Host SO PLOS ONE LA English DT Article ID A VIRUSES; WILD BIRDS; NORTH-AMERICA; SURVEILLANCE; PREVALENCE; WATERFOWL; ECOLOGY; DUCKS; PARAMYXOVIRUSES; TRANSMISSION AB Since the spread of highly pathogenic avian influenza (HPAI) H5N1 in the eastern hemisphere, numerous surveillance programs and studies have been undertaken to detect the occurrence, distribution, or spread of avian influenza viruses (AIV) in wild bird populations worldwide. To identify demographic determinants and spatiotemporal patterns of AIV infection in long distance migratory waterfowl in North America, we fitted generalized linear models with binominal distribution to analyze results from 13,574 blue-winged teal (Anas discors, BWTE) sampled in 2007 to 2010 year round during AIV surveillance programs in Canada and the United States. Our analyses revealed that during late summer staging (July-August) and fall migration (September-October), hatch year (HY) birds were more likely to be infected than after hatch year (AHY) birds, however there was no difference between age categories for the remainder of the year (winter, spring migration, and breeding period), likely due to maturing immune systems and newly acquired immunity of HY birds. Probability of infection increased non-linearly with latitude, and was highest in late summer prior to fall migration when densities of birds and the proportion of susceptible HY birds in the population are highest. Birds in the Central and Mississippi flyways were more likely to be infected compared to those in the Atlantic flyway. Seasonal cycles and spatial variation of AIV infection were largely driven by the dynamics of AIV infection in HY birds, which had more prominent cycles and spatial variation in infection compared to AHY birds. Our results demonstrate demographic as well as seasonal, latitudinal and flyway trends across Canada and the US, while illustrating the importance of migratory host life cycle and age in driving cyclical patterns of prevalence. C1 [Nallar, Rodolfo; Leighton, Frederick A.; Soos, Catherine] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK S7N 0W0, Canada. [Nallar, Rodolfo] Wildlife Conservat Soc, Greater Madidi Tambopata Landscape Conservat Prog, La Paz, Bolivia. [Papp, Zsuzsanna; Soos, Catherine] Environm Canada, Sci & Technol Branch, Saskatoon, SK, Canada. [Epp, Tasha] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. [Leighton, Frederick A.] Univ Saskatchewan, Canadian Wildlife Hlth Cooperat, Saskatoon, SK S7N 0W0, Canada. [Swafford, Seth R.] US Fish & Wildlife Serv, US Dept Interior, Yazoo City, MS USA. [DeLiberto, Thomas J.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Dis Program, Ft Collins, CO USA. [Dusek, Robert J.; Ip, Hon S.; Hall, Jeffrey] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Berhane, Yohannes] Natl Ctr Foreign Anim Dis, Canadian Food Inspect Agcy, Winnipeg, MB, Canada. [Gibbs, Samantha E. J.] US Fish & Wildlife Serv, Div Migratory Bird Management, Laurel, MD USA. RP Soos, C (reprint author), Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK S7N 0W0, Canada. EM catherine.soos@ec.gc.ca OI Hall, Jeffrey/0000-0001-5599-2826; Dusek, Robert/0000-0001-6177-7479 FU Alberta Conservation Association, Canada's Inter-agency Wild Bird Influenza Survey; Ducks Unlimited Canada's Institute for Wetland and Waterfowl Research, Environment Canada; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN266200700007C]; University of Saskatchewan; Wildlife Conservation Society; Canadian Food Inspection Agency; Public Health Agency of Canada; Environment Canada; participating provinces; USDA-APHIS FX This research was funded by the Alberta Conservation Association, Canada's Inter-agency Wild Bird Influenza Survey (which is funded by the Canadian Food Inspection Agency, Public Health Agency of Canada, Environment Canada, participating provinces, and USDA-APHIS), Ducks Unlimited Canada's Institute for Wetland and Waterfowl Research, Environment Canada, the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN266200700007C (USA), the University of Saskatchewan, the Wildlife Conservation Society (Graduate Fellowship Program). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 2 Z9 2 U1 5 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 25 PY 2015 VL 10 IS 6 AR e0130662 DI 10.1371/journal.pone.0130662 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL4OY UT WOS:000356933800071 PM 26110538 ER PT J AU Warren, KJ Olson, MM Thompson, NJ Cahill, ML Wyatt, TA Yoon, KJ Loiacono, CM Kohut, ML AF Warren, Kristi J. Olson, Molly M. Thompson, Nicholas J. Cahill, Mackenzie L. Wyatt, Todd A. Yoon, Kyoungjin J. Loiacono, Christina M. Kohut, Marian L. TI Exercise Improves Host Response to Influenza Viral Infection in Obese and Non-Obese Mice through Different Mechanisms SO PLOS ONE LA English DT Article ID DIET-INDUCED OBESITY; T-CELL RESPONSES; AMERICAN-HEART-ASSOCIATION; VIRUS-INFECTION; DENDRITIC CELLS; CARDIORESPIRATORY FITNESS; INSULIN-RESISTANCE; POSITIVE FEEDBACK; PHYSICAL-ACTIVITY; CLONAL EXPANSION AB Obesity has been associated with greater severity of influenza virus infection and impaired host defense. Exercise may confer health benefits even when weight loss is not achieved, but it has not been determined if regular exercise improves immune defense against influenza A virus (IAV) in the obese condition. In this study, diet-induced obese mice and lean control mice exercised for eight weeks followed by influenza viral infection. Exercise reduced disease severity in both obese and non-obese mice, but the mechanisms differed. Exercise reversed the obesity-associated delay in bronchoalveolar-lavage (BAL) cell infiltration, restored BAL cytokine and chemokine production, and increased ciliary beat frequency and IFN alpha-related gene expression. In non-obese mice, exercise treatment reduced lung viral load, increased Type-I-IFN-related gene expression early during infection, but reduced BAL inflammatory cytokines and chemokines. In both obese and non-obese mice, exercise increased serum anti-influenza virus specific IgG2c antibody, increased CD8+ T cell percentage in BAL, and reduced TNF alpha by influenza viral NP-peptide-responding CD8+ T cells. Overall, the results suggest that exercise "restores" the immune response of obese mice to a phenotype similar to non-obese mice by improving the delay in immune activation. In contrast, in non-obese mice exercise treatment results in an early reduction in lung viral load and limited inflammatory response. C1 [Warren, Kristi J.; Yoon, Kyoungjin J.; Kohut, Marian L.] Iowa State Univ, Immunobiol Program, Ames, IA 50011 USA. [Olson, Molly M.; Thompson, Nicholas J.; Cahill, Mackenzie L.; Kohut, Marian L.] Iowa State Univ, Coll Human Sci, Dept Kinesiol, Ames, IA USA. [Wyatt, Todd A.] VA Nebraska Western Iowa Hlth Care Syst, Res Serv, Dept Vet Affairs, Med Ctr, Omaha, NE USA. [Wyatt, Todd A.] Univ Nebraska, Med Ctr, Sleep & Allergy Div, Pulm Crit Care, Omaha, NE 68182 USA. [Loiacono, Christina M.] USDA, Natl Vet Serv Labs, APHIS, Ames, IA 50010 USA. [Yoon, Kyoungjin J.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. RP Kohut, ML (reprint author), Iowa State Univ, Immunobiol Program, Ames, IA 50011 USA. EM mkohut@iastate.edu FU National Institute of Allergy and Infectious Diseases at the National Institute of Health [R01 AI 059455-A2] FX This work was supported in part by the National Institute of Allergy and Infectious Diseases at the National Institute of Health [R01 AI 059455-A2] awarded to MLK. NR 60 TC 3 Z9 3 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 25 PY 2015 VL 10 IS 6 AR e0129713 DI 10.1371/journal.pone.0129713 PG 27 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL4OY UT WOS:000356933800028 PM 26110868 ER PT J AU Bai, YL Cui, Y Paoli, GC Shi, CL Wang, DP Shi, XM AF Bai, Yalong Cui, Yan Paoli, George C. Shi, Chunlei Wang, Dapeng Shi, Xianming TI Nanoparticles Affect PCR Primarily via Surface Interactions with PCR Components: Using Amino-Modified Silica-Coated Magnetic Nanoparticles as a Main Model SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE nanomaterials; polymerase chain reaction; effect; amino-modified silica-coated magnetic nanoparticles; gold nanoparticles ID POLYMERASE-CHAIN-REACTION; GOLD NANOPARTICLES; CARBON NANOTUBES; QUANTUM DOTS; SPECIFICITY; DNA; EFFICIENCY; IMMOBILIZATION; ADSORPTION; MECHANISM AB Nanomaterials have been widely reported to affect the polymerase chain reaction (PCR.). However, many studies in which these,effects were observed were not comprehensive, and many of the proposed mechanisms have been primarily speculative. In this work, we used amino-modified silica-coated magnetic nanoparticles,(ASMNPs, which can be collected very easily using an external magnetic field) as a model and compared them with gold nanoparticles (AuNPs, which have been studied extensively) to reveal the mechanisms by which nanoparticles affect PCR. We found that nanoparticles affect PCR primarily by binding to PCR components: (1) inhibition, (2) specifity, and (3) efficiency and yield of PCR are impacted. (1) Excess nanomaterials inhibit PCR by adsorbing to DNA polymerase, Mg2+, oligonucleotide primers, or DNA templates. Nanoparticle surface-active groups are particularly important to this effect. (2, a) Nanomaterials do not inhibit nonspecific amplification products caused by false priming as previously surmised. It was shown that relatively low concentrations of nanoparticles inhibited the amplification of long amplicons, and increasing the amount of nanoparticles, inhibited the amplification of short amplicons. This concentration phenomenon appears to be the result of the formation of "joints" upon the adsorption Of ASMNPs to DNA templates. (b) Nanomaterials are able to inhibit nonspecific amplification products due to incomplete amplification by preferably adsorbing, single-stranded incomplete amplification products. (3) Some types of nanomaterials, such as AuNPs, enhance the efficiency and yield of PCR because these types of nanoparticles can adsorb to single-stranded DNA more strongly than to double-stranded DNA. This behavior assists in the rapid and thorough denaturation of double-stranded DNA templates. Therefore, the interaction between the surface of nanoparticles and PCR components is sufficient to explain most of the effects of nanoparticles on PCR. C1 [Bai, Yalong; Cui, Yan; Shi, Chunlei; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. [Bai, Yalong; Cui, Yan; Shi, Chunlei; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Sch Agr & Biol, Shanghai 200240, Peoples R China. [Bai, Yalong; Cui, Yan; Shi, Chunlei; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China. [Paoli, George C.] ARS, USDA MOST Joint Res Ctr Food Safety, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Paoli, George C.] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Shi, XM (reprint author), Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. EM xmshi@sjtu.edu.cn FU Ministry of Science and Technology of China [2012AA101601, 2012BAD29B02]; National Natural Science Foundation of China [31230058] FX This study was supported by the Ministry of Science and Technology of China (2012AA101601 and 2012BAD29B02) and the National Natural Science Foundation of China (31230058). NR 51 TC 3 Z9 3 U1 8 U2 39 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD JUN 24 PY 2015 VL 7 IS 24 BP 13142 EP 13153 DI 10.1021/am508842v PG 12 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA CL6FO UT WOS:000357063200006 PM 26030087 ER PT J AU Jones, CR Brunner, JL Scoles, GA Owen, JP AF Jones, Cami R. Brunner, Jesse L. Scoles, Glen A. Owen, Jeb P. TI Factors affecting larval tick feeding success: host, density and time SO PARASITES & VECTORS LA English DT Article DE Parasite development; Host defense; Parasite density; Host-parasite interaction; Dermacentor andersoni; Peromyscus maniculatus ID MOUNTAIN WOOD TICK; LYME-DISEASE RISK; IXODES-SCAPULARIS; DERMACENTOR-ANDERSONI; ACQUIRED-RESISTANCE; RODENT HOSTS; BORRELIA-BURGDORFERI; POPULATION-DYNAMICS; PEROMYSCUS-LEUCOPUS; UNITED-STATES AB Background: Ectoparasites rely on blood-feeding to sustain activity, support development and produce offspring. Blood-feeding is also a route for transmission of diverse vector-borne pathogens. The likelihood of successfully feeding is thus an important aspect of ectoparasite population dynamics and pathogen transmission. Factors that affect blood-feeding include ectoparasite density, host defenses, and ages of the host and ectoparasite. How these factors interact to affect feeding success is not well understood. Methods: We monitored blood-feeding success of larval Rocky Mountain wood ticks (RMWTs; Dermacentor andersoni) on deer mice (Peromyscus maniculatus) in several experiments to determine how tick density, host defense, and ages of mice and ticks interact to influence feeding success. In the first experiment, tick-naive deer mice were infested with one of several densities of RMWT larvae, while a second cohort of mice were infested with 50 larvae each. Two weeks after ticks dropped off, mice in the first cohort were re-exposed to 50 larvae each and mice in the second cohort were re-exposed to varying densities of larvae. In the second experiment mice of different ages (45-374 days old) were exposed to 50 larvae each. Two weeks later mice were re-exposed to 50 larvae each. We combined data from these and several similar experiments to test the generality of the patterns we observed. Lastly, we tested whether tick feeding success was consistent on individual mice that were challenged on four occasions. Results: Mice acquired resistance such that feeding success declined dramatically from the first to the second infestation. Feeding success also declined with tick density and tick age. Mice, however, became more permissive with age. The sizes of these effects were similar and additive. Surprisingly, over successive infestations the relative resistance among mice changed among hosts within a cohort. Conclusions: We predict that larval blood-feeding success, and thus development to the nymph stage, will change due to variation in tick age and density, as well as the age and history of the host. Incorporating these biotic factors into modeling of tick population dynamics may improve predictions of tick-borne pathogen transmission. C1 [Jones, Cami R.; Owen, Jeb P.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Brunner, Jesse L.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Scoles, Glen A.] Washington State Univ, Anim Dis Unit, USDA ARS, Pullman, WA 99164 USA. RP Jones, CR (reprint author), Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. EM cami.jones@email.wsu.edu; jowen@wsu.edu FU Washington State University; USDA, ARS, Animal Disease Research Unit CRIS [5348-32000-033-00D] FX These experiments were made possible through funding from a Washington State University New Faculty SEED Grant, as well as USDA, ARS, Animal Disease Research Unit CRIS project number 5348-32000-033-00D. We would like to thank the animal facility staff, Ralph Horn and Kathy Mason for their help in animal care and for providing tick larvae. Special thanks to Samantha Whiteside for her help in animal care as well. NR 60 TC 2 Z9 2 U1 11 U2 27 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD JUN 24 PY 2015 VL 8 AR 340 DI 10.1186/s13071-015-0955-6 PG 10 WC Parasitology SC Parasitology GA CL6LO UT WOS:000357078800001 PM 26104393 ER PT J AU Collins, CDH Kautz, MA Tiller, R Lohani, S Ponce-Campos, G Hottenstein, J Metz, LJ AF Collins, Chandra D. Holifield Kautz, Mark A. Tiller, Ronald Lohani, Sapana Ponce-Campos, Guillermo Hottenstein, John Metz, Loretta J. TI Development of an integrated multiplatform approach for assessing brush management conservation efforts in semiarid rangelands (vol 9, 096057, 2015) SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Correction C1 [Collins, Chandra D. Holifield; Ponce-Campos, Guillermo] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Kautz, Mark A.; Lohani, Sapana; Hottenstein, John] Univ Arizona, Tucson, AZ 85721 USA. [Tiller, Ronald] Nature Conservancy, Tucson, AZ 85719 USA. [Metz, Loretta J.] USDA, Resource Assessment Div CEAP Grazing Lands, Grassland Soil & Water Res Lab, NRCS, Temple, TX 76502 USA. RP Collins, CDH (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. NR 1 TC 1 Z9 1 U1 1 U2 2 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD JUN 23 PY 2015 VL 9 AR 099999 DI 10.1117/1.JRS.9.099999 PG 1 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CM9BO UT WOS:000358000600001 ER PT J AU Neven, LG AF Neven, Lisa G. TI Reduction of Optimal Thermal Range in Aging Western Cherry Fruit Flies (Diptera: Tephritidae) SO JOURNAL OF INSECT SCIENCE LA English DT Article DE differential scanning calorimetry; microrespirometry; optimal thermal range; thermal tolerance ID RHAGOLETIS-INDIFFERENS DIPTERA; CODLING MOTH METABOLISM; FLY DIPTERA; DIAPAUSE DEVELOPMENT; FEEDING RESPONSES; TEMPERATURE; TOLERANCE; HEAT; ATMOSPHERES; WASHINGTON AB The western cherry fruit fly is an economically important pest of sweet cherries in the western United States. The potential of this pest to establish and spread in areas in which it is not currently present has been the focus of recent research. Most published information on the thermal tolerance and optimal thermal range of this pest has focused primarily on the diapausing pupae and predictive phenology models. Microrespirometry and differential calorimetry can be useful tools in describing the thermotolerance and optimal thermal range of insects. This methodology was employed to investigate the effects of western cherry fruit fly adult age on the optimal thermal range. Newly emerged flies exhibited the widest optimal thermal range spanning from 6.6 to 42.2 degrees C for a total range of 35.8 degrees C during heating scans of 0.4 degrees C/ min from 2 to 50 degrees C. This range diminished as the flies aged, with the shortest span observed with 28-d-old flies ranging from 10.5 to 37.8 degrees C, a span of 27.2 degrees C. Measurements of heat rate and oxygen consumption at isothermal, or static, temperatures indicated that all flies could survive exposure to 40 degrees C for at least 20 min, and that metabolism was greatly reduced, with a concomitant reduction in oxygen consumption rate at 40 to 42 degrees C. All flies exhibited a heat rate and oxygen consumption rate of zero when exposed to 45 and 50 degrees C. The loss of thermotolerance in adult flies can influence its ability to establish and spread in climates where daily temperatures exceed the optimal thermal range of this species. C1 USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Neven, LG (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM lisa.neven@ars.usda.gov FU Washington Tree Fruit Research Commission; USDA-Foreign Agricultural Service Technical Assistance Specialty Crops grants program FX I would like to thank Nathan Lehrman of Central Washington University, Janine Jewett and Jennifer Stout of the USDA-ARS-YARL for their technical assistance. This research was funded by a grant from the Washington Tree Fruit Research Commission in conjunction with the USDA-Foreign Agricultural Service Technical Assistance Specialty Crops grants program. I would also like to thank Dr. Rodney Cooper, USDA-ARS-YARL, and Dr. Sunil Kumar, Colorado State University, for peer reviews of this manuscript. NR 27 TC 0 Z9 0 U1 4 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUN 23 PY 2015 VL 15 AR 77 DI 10.1093/jisesa/iev059 PG 5 WC Entomology SC Entomology GA CM5IB UT WOS:000357720200005 ER PT J AU McMurray, RG Butte, NF Crouter, SE Trost, SG Pfeiffer, KA Bassett, DR Puyau, MR Berrigan, D Watson, KB Fulton, JE AF McMurray, Robert G. Butte, Nancy F. Crouter, Scott E. Trost, Stewart G. Pfeiffer, Karin A. Bassett, David R. Puyau, Maurice R. Berrigan, David Watson, Kathleen B. Fulton, Janet E. CA CDC NCI NCCOR Res Grp Energy Expen TI Exploring Metrics to Express Energy Expenditure of Physical Activity in Youth SO PLOS ONE LA English DT Article ID BODY-SIZE; SUBSTRATE UTILIZATION; AEROBIC POWER; CHILDREN; ADOLESCENTS; EXPLANATION; COMPENDIUM; VARIABLES; EXERCISE; ECONOMY AB Background Several approaches have been used to express energy expenditure in youth, but no consensus exists as to which best normalizes data for the wide range of ages and body sizes across a range of physical activities. This study examined several common metrics for expressing energy expenditure to determine whether one metric can be used for all healthy children. Such a metric could improve our ability to further advance the Compendium of Physical Activities for Youth. Methods A secondary analysis of oxygen uptake (VO2) data obtained from five sites was completed, that included 947 children ages 5 to 18 years, who engaged in 14 different activities. Resting metabolic rate (RMR) was computed based on Schofield Equations [Hum Nutr Clin Nut. 39 (Suppl 1), 1985]. Absolute oxygen uptake (ml.min(-1)), oxygen uptake per kilogram body mass (VO2 in ml. kg(-1).min(-1)), net oxygen uptake (VO2 - resting metabolic rate), allometric scaled oxygen uptake (VO2 in ml. kg(-0.75).min(-1)) and YOUTH-MET (VO2.[resting VO2](-1)) were calculated. These metrics were regressed with age, sex, height, and body mass. Results Net and allometric-scaled VO2, and YOUTH-MET were least associated with age, sex and physical characteristics. For moderate-to-vigorous intensity activities, allometric scaling was least related to age and sex. For sedentary and low-intensity activities, YOUTH-MET was least related to age and sex. Conclusions No energy expenditure metric completely eliminated the influence of age, physical characteristics, and sex. The Adult MET consistently overestimated EE. YOUTH-MET was better for expressing energy expenditure for sedentary and light activities, whereas allometric scaling was better for moderate and vigorous intensity activities. From a practical perspective, The YOUTH-MET may be the more feasible metric for improving of the Compendium of Physical Activities for Youth. C1 [McMurray, Robert G.] Univ N Carolina, Exercise & Sport Sci & Nutr, Chapel Hill, NC 27599 USA. [Butte, Nancy F.; Puyau, Maurice R.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Crouter, Scott E.; Bassett, David R.] Univ Tennessee, Dept Kinesiol Recreat & Sport Studies, Knoxville, TN USA. [Trost, Stewart G.] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Exercise & Nutr Sci, Brisbane, Qld 4001, Australia. [Pfeiffer, Karin A.] Michigan State Univ, Dept Kinesiol, E Lansing, MI 48824 USA. [Berrigan, David] NCI, Ctr Dis Control & Prevent, Natl Collaborat Childhood Obes Res, Washington, DC USA. [Watson, Kathleen B.; Fulton, Janet E.] CDC, Div Nutr Phys Act & Obes, Atlanta, GA 30333 USA. RP McMurray, RG (reprint author), Univ N Carolina, Exercise & Sport Sci & Nutr, Chapel Hill, NC 27599 USA. EM exphys@live.unc.edu RI Crouter, Scott/H-4852-2011; OI Crouter, Scott/0000-0003-1297-9538; Trost, Stewart/0000-0001-9587-3944 FU NHLBI NIH HHS [R21 HL093407]; NICHD NIH HHS [L30 HD066736] NR 32 TC 2 Z9 2 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 23 PY 2015 VL 10 IS 6 AR e0130869 DI 10.1371/journal.pone.0130869 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL4DD UT WOS:000356901900064 PM 26102204 ER PT J AU Zhao, LS Wu, FQ AF Zhao, Longshan Wu, Faqi TI Simulation of Runoff Hydrograph on Soil Surfaces with Different Microtopography Using a Travel Time Method at the Plot Scale SO PLOS ONE LA English DT Article ID OVERLAND-FLOW; ROUGHNESS; INFILTRATION; DEPRESSION; MODEL; WATER; GIS; RAINFALL; STORAGE AB In this study, a simple travel time-based runoff model was proposed to simulate a runoff hydrograph on soil surfaces with different microtopographies. Three main parameters, i.e., rainfall intensity (I), mean flow velocity (v(m)) and ponding time of depression (t(p)), were inputted into this model. The soil surface was divided into numerous grid cells, and the flow length of each grid cell (l(i)) was then calculated from a digital elevation model (DEM). The flow velocity in each grid cell (v(i)) was derived from the upstream flow accumulation area using v(m). The total flow travel time through each grid cell to the surface outlet was the sum of the sum of flow travel times along the flow path (i.e., the sum of l(i)/v(i)) and t(p). The runoff rate at the slope outlet for each respective travel time was estimated by finding the sum of the rain rate from all contributing cells for all time intervals. The results show positive agreement between the measured and predicted runoff hydrographs. C1 [Zhao, Longshan; Wu, Faqi] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling, Shaanxi, Peoples R China. [Zhao, Longshan] Guizhou Univ, Coll Forestry, Guiyang 550003, Guizhou, Peoples R China. [Zhao, Longshan] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Zhao, LS (reprint author), Northwest A&F Univ, Coll Nat Resources & Environm, Yangling, Shaanxi, Peoples R China. EM zls@nwsuaf.edu.cn FU National Natural Science Foundation of China [41271288] FX This study was funded by the National Natural Science Foundation of China (No. 41271288). NR 24 TC 0 Z9 0 U1 1 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 23 PY 2015 VL 10 IS 6 AR e0130794 DI 10.1371/journal.pone.0130794 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL4DD UT WOS:000356901900061 PM 26103635 ER PT J AU Bellaloui, N Turley, RB Stetina, SR AF Bellaloui, Nacer Turley, Rickie B. Stetina, Salliana R. TI Water Stress and Foliar Boron Application Altered Cell Wall Boron and Seed Nutrition in Near-Isogenic Cotton Lines Expressing Fuzzy and Fuzzless Seed Phenotypes SO PLOS ONE LA English DT Article ID GOSSYPIUM-HIRSUTUM L.; CHANNEL-MEDIATED TRANSPORT; FATTY-ACID-COMPOSITION; RHAMNOGALACTURONAN-II; IRRIGATION REGIMES; PLANT AQUAPORINS; BORIC-ACID; QUALITY; REQUIREMENT; SQUASH AB Our previous research, conducted under well-watered conditions without fertilizer application, showed that fuzziness cottonseed trait resulted in cottonseed nutrition differences between fuzzy (F) and fuzzless (N) cottonseed. Under water stress conditions, B mobility is further limited, inhibiting B movement within the plant, affecting seed nutrition (quality). Therefore, we hypothesized that both foliar B and water stress can affect B mobility, altering cottonseed protein, oil, and mineral nutrition. The objective of the current research was to evaluate the effects of the fuzziness seed trait on boron (B) and seed nutrition under water stress and foliar B application using near-isogenic cotton lines (NILs) grown in a repeated greenhouse experiment. Plants were grown under-well watered conditions (The soil water potential was kept between -15 to -20 kPa, considered field capacity) and water stress conditions (soil water potential between -100 and -150 kPa, stressed conditions). Foliar B was applied at a rate of 1.8 kg B ha(-1) as H3BO3. Under well-watered conditions without B the concentrations of seed oil in N lines were higher than in F lines, and seed K and N levels were lower in N lines than in Flines. Concentrations of K, N, and B in leaves were higher in Nlines than in Flines, opposing the trend in seeds. Water-stress resulted in higher seed protein concentrations, and the contribution of cell wall (structural) B to the total B exceeded 90%, supporting the structural role of B in plants. Foliar B application under well-watered conditions resulted in higher seed protein, oil, C, N, and B in only some lines. This research showed that cottonseed nutrition differences can occur due to seed fuzziness trait, and water stress and foliar B application can alter cottonseed nutrition. C1 [Bellaloui, Nacer; Turley, Rickie B.; Stetina, Salliana R.] ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38776 USA. RP Bellaloui, N (reprint author), ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38776 USA. EM nacer.bellaloui@ars.usda.gov FU USDA, Agricultural Research Service [6066-21220-012-00D, 6066-21000-051-00D] FX Funding for this research was provided by USDA, Agricultural Research Service projects 6066-21220-012-00D and 6066-21000-051-00D (http://www.ars.usda.gov/pandp/people/projects.htm?personid=37050). NR 62 TC 0 Z9 0 U1 1 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 22 PY 2015 VL 10 IS 6 AR e0130759 DI 10.1371/journal.pone.0130759 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL3FM UT WOS:000356835800101 PM 26098564 ER PT J AU Ghattas, H Sassine, AJ Seyfert, K Nord, M Sahyoun, NR AF Ghattas, Hala Sassine, AnnieBelle J. Seyfert, Karin Nord, Mark Sahyoun, Nadine R. TI Prevalence and Correlates of Food Insecurity among Palestinian Refugees in Lebanon: Data from a Household Survey SO PLOS ONE LA English DT Article ID MENTAL-HEALTH; POPULATIONS AB Lebanon hosts the highest per capita refugee concentration worldwide. The Palestinian presence in Lebanon dates from 1948 and they remain a marginalized population. No information on their food security status has been reported previously. A survey of a representative sample of Palestinian refugee households in Lebanon (n = 2501) was conducted using a stratified two stage cluster sampling approach. We measured food insecurity using a modified USDA household food security module, locally validated. We collected data on household demographic, socioeconomic, health, housing, coping strategies and household intake of food groups and analysed these by food security status. About 41% (CI: 39-43) of households reported being food insecure and 20% (CI: 18-22) severely food insecure. Poor households were more likely to be severely food insecure (OR 1.41 (1.06-1.86)) while higher education of the head of household was significantly associated with protection against severe food insecurity (OR 0.66 (0.52-0.84)). Additionally, higher food expenditure and possession of food-related assets were significantly associated with food security (OR 0.93 (0.89-0.97) and OR 0.74 (0.59-0.92), respectively). After adjusting for confounders, households where at least one member suffered from an acute illness remained significantly more likely to be severely food insecure (OR 1.31(1.02-1.66)), as were households whose proxy respondent reported poor mental health (OR 2.64 (2.07-3.38)) and poor self-reported health (OR 1.62 (1.22-2.13). Severely food insecure households were more likely to eat cheaper foods when compared to non-severely food insecure households (p<0.001) and were more likely to rely on gifts (p<0.001) or welfare (p<0.001). They were also more likely to have exhausted all coping strategies, indicating significantly more frequently that they could not do anything (p = 0.0102). Food insecurity is a significant problem among Palestinian refugees in Lebanon and is likely to be exacerbated at this time when the Syrian crisis amplifies the problem. C1 [Ghattas, Hala; Sassine, AnnieBelle J.] Amer Univ Beirut, Ctr Res Populat & Hlth, Beirut, Lebanon. [Seyfert, Karin] Univ London Sch Oriental & African Studies, Dept Econ, London, England. [Nord, Mark] ERS, USDA, Washington, DC USA. [Sahyoun, Nadine R.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Ghattas, H (reprint author), Amer Univ Beirut, Ctr Res Populat & Hlth, Beirut, Lebanon. EM hg15@aub.edu.lb RI Ghattas, Hala/K-5661-2016 FU European Union [SC-A-33/R082/000] FX This study was funded by the European Union through a grant to the United Nations Relief and Works Agency for Palestine Refugees in the Near East (UNRWA) SC-A-33/R082/000. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 22 TC 2 Z9 2 U1 3 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 22 PY 2015 VL 10 IS 6 AR e0130724 DI 10.1371/journal.pone.0130724 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL3FM UT WOS:000356835800098 PM 26098108 ER PT J AU Liu, N Liu, ZL Gong, GS Zhang, M Wang, X Zhou, Y Qi, XB Chen, HB Yang, JZ Luo, PG Yang, CP AF Liu, Na Liu, Z. Lewis Gong, Guoshu Zhang, Min Wang, Xu Zhou, You Qi, Xiaobo Chen, Huabao Yang, Jizhi Luo, Peigao Yang, Chunping TI Virulence Structure of Blumeria graminis f. sp tritici and Its Genetic Diversity by ISSR and SRAP Profiling Analyses SO PLOS ONE LA English DT Article ID WHEAT POWDERY MILDEW; AMPLIFIED POLYMORPHISM SRAP; MARKERS; POPULATIONS; RAPD; CHINA; ACCESSIONS; RESISTANCE; CULTIVARS; BRASSICA AB Blumeria graminis f. sp. tritici, which causes wheat powdery mildew, is an obligate biotrophic pathogen that can easily genetically adapt to its host plant. Understanding the virulence structure of and genetic variations in this pathogen is essential for disease control and for breeding resistance to wheat powdery mildew. This study investigated 17 pathogenic populations in Sichuan, China and classified 109 isolates into two distinct groups based on pathogenicity analysis: high virulence (HV, 92 isolates) and low virulence (LV, 17 isolates). Populations from Yibin (Southern region), Xichang (Western region), and Meishan (Middle region) showed lower virulence frequencies than populations from other regions. Many of the previously known resistance genes did not confer resistance in this study. The resistance gene Pm21 displayed an immune response to pathogenic challenge with all populations in Sichuan, and Pm13, Pm5b, Pm2+6, and PmXBD maintained resistance. AMOVA revealed significantly higher levels of variation within populations and lower levels of variation among populations within regions. High levels of gene flow were detected among populations in the four regions. Closely related populations within each region were distinguished by cluster analyses using ISSR and SRAP alleles. Both ISSR and SRAP allele profiling analyses revealed high levels of genetic diversity among pathogenic populations in Sichuan. Although ISSR and SRAP profiling analysis showed similar resolutions, the SRAP alleles appeared to be more informative. We did not detect any significant association between these alleles and the virulence or pathogenicity of the pathogen. Our results suggest that ISSR and SRAP alleles are more efficient for the characterization of small or closely related populations versus distantly related populations. C1 [Liu, Na; Gong, Guoshu; Zhang, Min; Zhou, You; Qi, Xiaobo; Chen, Huabao; Yang, Jizhi; Luo, Peigao; Yang, Chunping] Sichuan Agr Univ, Coll Agron, Chengdu, Sichuan, Peoples R China. [Liu, Z. Lewis] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. [Wang, Xu] Sichuan Agr Univ, Coll Resources, Chengdu, Sichuan, Peoples R China. RP Gong, GS (reprint author), Sichuan Agr Univ, Coll Agron, Chengdu, Sichuan, Peoples R China. EM guoshugong@126.com FU Public Welfare Specific Foundation of Agronomy, China [201303016] FX This work was supported by the Public Welfare Specific Foundation of Agronomy (No. 201303016), China. NR 67 TC 0 Z9 1 U1 1 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 22 PY 2015 VL 10 IS 6 AR e0130881 DI 10.1371/journal.pone.0130881 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL3FM UT WOS:000356835800116 PM 26098844 ER PT J AU Mousel, MR Reynolds, JO White, SN AF Mousel, Michelle R. Reynolds, James O. White, Stephen N. TI Genome-Wide Association Identifies SLC2A9 and NLN Gene Regions as Associated with Entropion in Domestic Sheep SO PLOS ONE LA English DT Article ID CONGENITAL ENTROPION; EYELID DEVELOPMENT; INVOLUTIONAL ECTROPION; ENDOTHELIAL-CELLS; URATE TRANSPORTER; SKELETAL-MUSCLE; HISTOPATHOLOGY; POPULATION; EXPRESSION; MECHANISM AB Entropion is an inward rolling of the eyelid allowing contact between the eyelashes and cornea that may lead to blindness if not corrected. Although many mammalian species, including humans and dogs, are afflicted by congenital entropion, no specific genes or gene regions related to development of entropion have been reported in any mammalian species to date. Entropion in domestic sheep is known to have a genetic component therefore, we used domestic sheep as a model system to identify genomic regions containing genes associated with entropion. A genome-wide association was conducted with congenital entropion in 998 Columbia, Polypay, and Rambouillet sheep genotyped with 50,000 SNP markers. Prevalence of entropion was 6.01%, with all breeds represented. Logistic regression was performed in PLINK with additive allelic, recessive, dominant, and genotypic inheritance models. Two genome-wide significant (empirical P<0.05) SNP were identified, specifically markers in SLC2A9 (empirical P = 0.007; genotypic model) and near NLN (empirical P = 0.026; dominance model). Six additional genome-wide suggestive SNP (nominal P<1x10(-5)) were identified including markers in or near PIK3CB (P = 2.22x10(-6); additive model), KCNB1 (P = 2.93x10(-6); dominance model), ZC3H12C (P = 3.25x10(-6); genotypic model), JPH1 (P = 4.68x20(-6); genotypic model), and MYO3B (P = 5.74x10(-6); recessive model). This is the first report of specific gene regions associated with congenital entropion in any mammalian species, to our knowledge. Further, none of these genes have previously been associated with any eyelid traits. These results represent the first genome-wide analysis of gene regions associated with entropion and provide target regions for the development of sheep genetic markers for marker-assisted selection. C1 [Mousel, Michelle R.] ARS, Range Sheep Prod Efficiency Res Unit, USDA, Dubois, ID USA. [Reynolds, James O.; White, Stephen N.] ARS, USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. [White, Stephen N.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP Mousel, MR (reprint author), ARS, USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. EM Michelle.Mousel@ars.usda.gov OI Mousel, Michelle/0000-0003-1367-7005 FU USDA-ARS CWA [5348-32000-031-00D] FX This research was funded by USDA-ARS CWA No. 5348-32000-031-00D (http://www.ars.usda.gov/main/main.htm). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 0 Z9 0 U1 1 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 22 PY 2015 VL 10 IS 6 AR e0128909 DI 10.1371/journal.pone.0128909 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL3FM UT WOS:000356835800020 PM 26098909 ER PT J AU Goyer, A Hamlin, L Crosslin, JM Buchanan, A Chang, JH AF Goyer, Aymeric Hamlin, Launa Crosslin, James M. Buchanan, Alex Chang, Jeff H. TI RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection SO BMC GENOMICS LA English DT Article DE Potato virus Y; Potato; RNA-Seq; Leaf; Resistance ID MOTIF-CONTAINING PROTEINS; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; DEFENSE RESPONSES; SALICYLIC-ACID; PHYTOPHTHORA-INFESTANS; TRANSCRIPTION FACTOR; DIFFERENTIAL GENE; PATHOGENS; PLANTS AB Background: Potato virus Y (PVY) is one of the most important plant viruses affecting potato production. The interactions between potato and PVY are complex and the outcome of the interactions depends on the potato genotype, the PVY strain, and the environmental conditions. A potato cultivar can induce resistance to a specific PVY strain, yet be susceptible to another. How a single potato cultivar responds to PVY in both compatible and incompatible interactions is not clear. Results: In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare the transcriptional changes in leaves of potato upon inoculation with PVY. We used two potato varieties: Premier Russet, which is resistant to the PVY strain O (PVYO) but susceptible to the strain NTN (PVYNTN), and Russet Burbank, which is susceptible to all PVY strains that have been tested. Leaves were inoculated with PVYO or PVYNTN, and samples were collected 4 and 10 h post inoculation (hpi). A larger number of differentially expressed (DE) genes were found in the compatible reactions compared to the incompatible reaction. For all treatments, the majority of DE genes were down-regulated at 4 hpi and up-regulated at 10 hpi. Gene Ontology enrichment analysis showed enrichment of the biological process GO term "Photosynthesis, light harvesting" specifically in PVYO-inoculated Premier Russet leaves, while the GO term "nucleosome assembly" was largely overrepresented in PVYNTN-inoculated Premier Russet leaves and PVYO-inoculated Russet Burbank leaves but not in PVYO-inoculated Premier Russet leaves. Fewer genes were DE over 4-fold in the incompatible reaction compared to the compatible reactions. Amongst these, five genes were DE only in PVYO-inoculated Premier Russet leaves, and all five were down-regulated. These genes are predicted to encode for a putative ABC transporter, a MYC2 transcription factor, a VQ-motif containing protein, a non-specific lipid-transfer protein, and a xyloglucan endotransglucosylase-hydroxylase. Conclusions: Our results show that the incompatible and compatible reactions in Premier Russet shared more similarities, in particular during the initial response, than the compatible reactions in the two different hosts. Our results identify potential key processes and genes that determine the fate of the reaction, compatible or incompatible, between PVY and its host. C1 [Goyer, Aymeric; Buchanan, Alex; Chang, Jeff H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Goyer, Aymeric] Hermiston Agr Res & Extens Ctr, Hermiston, OR USA. [Goyer, Aymeric; Chang, Jeff H.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Hamlin, Launa; Crosslin, James M.] USDA ARS, Prosser, WA 99350 USA. RP Goyer, A (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM aymeric.goyer@oregonstate.edu FU Oregon potato commission; National Institute of General Medical Sciences of the National Institutes of Health [R01GM104977]; Oregon State University Libraries & Press Open Access Fund; Washington state potato commission FX We thank Dr. Yanming Di (Statistics Department, Oregon State University) for his advice with statistical design and analysis. This project was supported by funding from the Oregon and Washington state potato commissions. Work in the laboratory of JHC is supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM104977. Publication of this article was funded by the Oregon State University Libraries & Press Open Access Fund. NR 46 TC 4 Z9 4 U1 7 U2 44 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 20 PY 2015 VL 16 AR 472 DI 10.1186/s12864-015-1666-2 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CK8HM UT WOS:000356479100002 PM 26091899 ER PT J AU Hawkins, LK Mylroie, JE Oliveira, DA Smith, JS Ozkan, S Windham, GL Williams, WP Warburton, ML AF Hawkins, Leigh K. Mylroie, J. Erik Oliveira, Dafne A. Smith, J. Spencer Ozkan, Seval Windham, Gary L. Williams, W. Paul Warburton, Marilyn L. TI Characterization of the Maize Chitinase Genes and Their Effect on Aspergillus flavus and Aflatoxin Accumulation Resistance SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCI; DIFFERENTIAL EXPRESSION; CONTRIBUTING RESISTANCE; CHITINOLYTIC ENZYMES; PLANT CHITINASES; IDENTIFICATION; INFECTION; DATABASE; PROTEIN; GENOME AB Maize (Zea mays L.) is a crop of global importance, but prone to contamination by aflatoxins produced by fungi in the genus Aspergillus. The development of resistant germplasm and the identification of genes contributing to resistance would aid in the reduction of the problem with a minimal need for intervention by farmers. Chitinolytic enzymes respond to attack by potential pathogens and have been demonstrated to increase insect and fungal resistance in plants. Here, all chitinase genes in the maize genome were characterized via sequence diversity and expression patterns. Recent evolution within this gene family was noted. Markers from within each gene were developed and used to map the phenotypic effect on resistance of each gene in up to four QTL mapping populations and one association panel. Seven chitinase genes were identified that had alleles associated with increased resistance to aflatoxin accumulation and A. flavus infection in field grown maize. The chitinase in bin 1.05 identified a new and highly significant QTL, while chitinase genes in bins 2.04 and 5.03 fell directly beneath the peaks of previously published QTL. The expression patterns of these genes corroborate possible grain resistance mechanisms. Markers from within the gene sequences or very closely linked to them are presented to aid in the use of marker assisted selection to improve this trait. C1 [Hawkins, Leigh K.; Mylroie, J. Erik; Smith, J. Spencer; Windham, Gary L.; Williams, W. Paul; Warburton, Marilyn L.] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Oliveira, Dafne A.; Ozkan, Seval] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Starkville, MS 39762 USA. RP Hawkins, LK (reprint author), USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. EM Leigh.Hawkins@ars.usda.gov FU USDA; USDA/USAID Feed the Future initiative project [58-0210-3-0012] FX This work was supported by the USDA (http://www.ars.usda.gov/main/site_main.htm? modecode=64-06-15-05) and the joint USDA/USAID Feed the Future initiative project number 58-0210-3-0012 (www.usaid.gov). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 2 Z9 3 U1 4 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 19 PY 2015 VL 10 IS 6 AR e0126185 DI 10.1371/journal.pone.0126185 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL3FE UT WOS:000356835000007 PM 26090679 ER PT J AU Morales-Cruz, A Amrine, KCH Blanco-Ulate, B Lawrence, DP Travadon, R Rolshausen, PE Baumgartner, K Cantu, D AF Morales-Cruz, Abraham Amrine, Katherine C. H. Blanco-Ulate, Barbara Lawrence, Daniel P. Travadon, Renaud Rolshausen, Philippe E. Baumgartner, Kendra Cantu, Dario TI Distinctive expansion of gene families associated with plant cell wall degradation, secondary metabolism, and nutrient uptake in the genomes of grapevine trunk pathogens SO BMC GENOMICS LA English DT Article DE Comparative genomics; Computational Analysis of gene Family Evolution (CAFE); CAZymes; Peroxidases; Secondary metabolism; P450s ID ESCA-ASSOCIATED FUNGI; VITIS-VINIFERA L; EUTYPA-LATA; WOOD DECAY; NECTRIA-HAEMATOCOCCA; PHOMOPSIS-VITICOLA; PROTEIN FAMILIES; ERYSIPHE-NECATOR; CAZY DATABASE; RNA-SEQ AB Background: Trunk diseases threaten the longevity and productivity of grapevines in all viticulture production systems. They are caused by distantly-related fungi that form chronic wood infections. Variation in wood-decay abilities and production of phytotoxic compounds are thought to contribute to their unique disease symptoms. We recently released the draft sequences of Eutypa lata, Neofusicoccum parvum and Togninia minima, causal agents of Eutypa dieback, Botryosphaeria dieback and Esca, respectively. In this work, we first expanded genomic resources to three important trunk pathogens, Diaporthe ampelina, Diplodia seriata, and Phaeomoniella chlamydospora, causal agents of Phomopsis dieback, Botryosphaeria dieback, and Esca, respectively. Then we integrated all currently-available information into a genome-wide comparative study to identify gene families potentially associated with host colonization and disease development. Results: The integration of RNA-seq, comparative and ab initio approaches improved the protein-coding gene prediction in T. minima, whereas shotgun sequencing yielded nearly complete genome drafts of Dia. ampelina, Dip. seriata, and P. chlamydospora. The predicted proteomes of all sequenced trunk pathogens were annotated with a focus on functions likely associated with pathogenesis and virulence, namely (i) wood degradation, (ii) nutrient uptake, and (iii) toxin production. Specific patterns of gene family expansion were described using Computational Analysis of gene Family Evolution, which revealed lineage-specific evolution of distinct mechanisms of virulence, such as specific cell wall oxidative functions and secondary metabolic pathways in N. parvum, Dia. ampelina, and E. lata. Phylogenetically-informed principal component analysis revealed more similar repertoires of expanded functions among species that cause similar symptoms, which in some cases did not reflect phylogenetic relationships, thereby suggesting patterns of convergent evolution. Conclusions: This study describes the repertoires of putative virulence functions in the genomes of ubiquitous grapevine trunk pathogens. Gene families with significantly faster rates of gene gain can now provide a basis for further studies of in planta gene expression, diversity by genome re-sequencing, and targeted reverse genetic approaches. The functional validation of potential virulence factors will lead to a more comprehensive understanding of the mechanisms of pathogenesis and virulence, which ultimately will enable the development of accurate diagnostic tools and effective disease management. C1 [Morales-Cruz, Abraham; Amrine, Katherine C. H.; Blanco-Ulate, Barbara; Cantu, Dario] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Lawrence, Daniel P.; Travadon, Renaud] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Rolshausen, Philippe E.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Baumgartner, Kendra] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Cantu, D (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM dacantu@ucdavis.edu RI Cantu, Dario /E-7658-2010 OI Cantu, Dario /0000-0002-4858-1508 FU Horticulture & Agronomy graduate fellowship (UC Davis); Horace O. Lanza Scholarship; Wine Spectator Scholarship; Pearl & Albert J. Winkler Scholarship in Viticulture; USDA, National Institute of Food and Agriculture, Specialty Crop Research Initiative [2012-51181-19954] FX This research was funded by the USDA, National Institute of Food and Agriculture, Specialty Crop Research Initiative (grant number 2012-51181-19954). AMC was also partially supported by a Horticulture & Agronomy graduate fellowship (UC Davis), a Horace O. Lanza Scholarship, a Wine Spectator Scholarship, and a The Pearl & Albert J. Winkler Scholarship in Viticulture. NR 129 TC 11 Z9 11 U1 8 U2 60 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 19 PY 2015 VL 16 AR 469 DI 10.1186/s12864-015-1624-z PG 22 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CK8HL UT WOS:000356479000001 PM 26084502 ER PT J AU Martcheva, M Lenhart, S Eda, S Klinkenberg, D Momotani, E Stabel, J AF Martcheva, Maia Lenhart, Suzanne Eda, Shigetoshi Klinkenberg, Don Momotani, Eiichi Stabel, Judy TI An immuno-epidemiological model for Johne's disease in cattle SO VETERINARY RESEARCH LA English DT Article ID AVIUM SUBSPECIES PARATUBERCULOSIS; BETWEEN-HOST DYNAMICS; COUPLING WITHIN-HOST; MYCOBACTERIUM-PARATUBERCULOSIS; INFECTIOUS-DISEASE; DAIRY HERDS; CALVES; MACROPHAGES; EVOLUTION; COWS AB To better understand the mechanisms involved in the dynamics of Johne's disease in dairy cattle, this paper illustrates a novel way to link a within-host model for Mycobacterium avium ssp. paratuberculosis with an epidemiological model. The underlying variable in the within-host model is the time since infection. Two compartments, infected macrophages and T cells, of the within-host model feed into the epidemiological model through the direct transmission rate, disease-induced mortality rate, the vertical transmission rate, and the shedding of MAP into the environment. The epidemiological reproduction number depends on the within-host bacteria load in a complex way, exhibiting multiple peaks. A possible mechanism to account for the switch in shedding patterns of the bacteria in this disease is included in the within-host model, and its effect can be seen in the epidemiological reproduction model. C1 [Martcheva, Maia] Univ Florida, Dept Math, Gainesville, FL 32611 USA. [Lenhart, Suzanne] Univ Tennessee, Dept Math, Knoxville, TN 37996 USA. [Eda, Shigetoshi] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Klinkenberg, Don] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, NL-3584 CL Utrecht, Netherlands. [Momotani, Eiichi] Tokyo Med & Dent Univ, Tohto Coll Hlth Sci, Dept Human Care, Fukaya, Saitama 3660052, Japan. [Stabel, Judy] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Klinkenberg, Don] Natl Inst Publ Hlth & Environm, Ctr Infect Dis Control, NL-3720 BA Bilthoven, Netherlands. RP Lenhart, S (reprint author), Univ Tennessee, Dept Math, Knoxville, TN 37996 USA. EM lenhart@math.utk.edu FU Within-host modeling of MAP infections Working Group at the National Institute for Mathematical and Biological Synthesis; National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF [DBI-1300426]; University of Tennessee, Knoxville; University of Tennessee Center for Business and Economic Research FX The authors acknowledge the support of the Within-host modeling of MAP infections Working Group at the National Institute for Mathematical and Biological Synthesis, sponsored by the National Science Foundation, the U.S. Department of Homeland Security, and the U.S. Department of Agriculture through NSF Award DBI-1300426, with additional support from The University of Tennessee, Knoxville. Lenhart is partially supported by the University of Tennessee Center for Business and Economic Research. NR 40 TC 1 Z9 1 U1 1 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD JUN 19 PY 2015 VL 46 AR 69 DI 10.1186/s13567-015-0190-3 PG 13 WC Veterinary Sciences SC Veterinary Sciences GA CK9WK UT WOS:000356590000001 PM 26091672 ER PT J AU Schukken, YH Whitlock, RH Wolfgang, D Grohn, Y Beaver, A VanKessel, J Zurakowski, M Mitchell, R AF Schukken, Ynte H. Whitlock, Robert H. Wolfgang, Dave Grohn, Yrjo Beaver, Annabelle VanKessel, JoAnn Zurakowski, Mike Mitchell, Rebecca TI Longitudinal data collection of Mycobacterium avium subspecies Paratuberculosis infections in dairy herds: the value of precise field data SO VETERINARY RESEARCH LA English DT Article ID JOHNES-DISEASE STATUS; UNITED-STATES; CATTLE; COWS; TRANSMISSION; METAANALYSIS; AGGREGATION; ASSOCIATION; POPULATIONS; PATTERNS AB Longitudinal infection data on Mycobacterium avium subspecies paratuberculosis (MAP) was collected on three dairy farms in Northeastern United States during approximately 10 years. Precise data on animal characteristics and animal location within farm were collected on these farms. Cows were followed over time with regard to MAP status during biannual fecal and serum sampling and quarterly serum sampling. Approximately 13 000 serum samples, 6500 fecal samples and 2000 tissue samples were collected during these years. Prevalence of positive samples was 1.4% for serological samples, 2.2% in fecal samples and 16.7% in tissue samples. Infection dynamics of MAP was studied and resulted in a number of potential changes in our understanding of MAP infection dynamics. First, a high prevalence of MAP infection was observed in these herds due to lifetime follow up of cows, including slaughter. Second, two distinctly different infection patterns were observed, so called non-progressors and progressors. Non-progressors were characterized by intermittent and low shedding of MAP bacteria and a virtual absence of a humoral immune response. Progressors were characterized by continuous and progressive shedding and a clearly detectable and progressive humoral immune response. Strain typing of MAP isolates on the three farms identified on two of three farms a dominant strain type, indicating that some strains are more successful in terms of transmission and infection progression. Continuous high quality longitudinal data collection turned out to be an essential tool in our understanding of pathobiology and epidemiology of MAP infections in dairy herds. C1 [Schukken, Ynte H.; Grohn, Yrjo; Beaver, Annabelle; Zurakowski, Mike; Mitchell, Rebecca] Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. [Whitlock, Robert H.] Univ Penn, New Bolton Ctr, Kennett Sq, PA USA. [Wolfgang, Dave] Penn State Univ, Dept Vet Sci, University Pk, PA 16801 USA. [VanKessel, JoAnn] ARS, USDA, Baltimore, MD USA. [Schukken, Ynte H.] GD Anim Hlth, Deventer, Netherlands. RP Schukken, YH (reprint author), Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. EM y.schukken@gdanimalhealth.com OI Schukken, Ynte/0000-0002-8250-4194 FU National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF Award [DBI-1300426]; University of Tennessee, Knoxville FX The authors acknowledge the collaboration of the three dairy farmers and their staff within the Regional Dairy Quality Management Alliance. Without their input and support, the data collection in this study would not have been possible. The authors also acknowledge the support of the Within-host modeling of MAP infections Working Group at the National Institute for Mathematical and Biological Synthesis, sponsored by the National Science Foundation, the U.S. Department of Homeland Security, and the U.S. Department of Agriculture through NSF Award DBI-1300426, with additional support from The University of Tennessee, Knoxville. NR 36 TC 5 Z9 5 U1 1 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD JUN 19 PY 2015 VL 46 AR 65 DI 10.1186/s13567-015-0187-y PG 11 WC Veterinary Sciences SC Veterinary Sciences GA CK9WF UT WOS:000356589500001 PM 26092492 ER PT J AU Bicha, W Schiff, N Lancaster, A Scheffler, B AF Bicha, Wesley Schiff, Nathan Lancaster, Aaron Scheffler, Brian TI A nearly cryptic Scorpionfly, Panorpa cryptica n. sp (Mecoptera: Panorpidae) from North America SO ZOOTAXA LA English DT Article DE COI; DNA; Georgia; new species; North Carolina; Panorpa; Panorpa acuta; Panorpa flexa; Panorpa nebulosa; scorpionfly; South Carolina ID PRIMERS AB The first nearly cryptic species of scorpionfly from the United States, Panorpa cryptica Bicha and Schiff, n. sp., is described from northern Georgia, southwestern North Carolina and northwestern South Carolina. This insect was initially differentiated from the very similar Panorpa nebulosa Westwood by its unique cytochrome oxidase subunit I (COI) mitochondrial DNA. Habitat details, distribution, and biology are described. C1 [Schiff, Nathan; Lancaster, Aaron] USDA, Ctr Bottomland Hardwoods Res, Forest Serv, Stoneville, MS 38776 USA. [Scheffler, Brian] USDA ARS, CGRU, MSA Genom Lab, Stoneville, MS 38776 USA. RP Bicha, W (reprint author), 121 Old Batley Rd, Oliver Springs, TN 37840 USA. EM mecoptera@live.com; nschiff@fs.fed.us; brian.scheffler@ars.usda.gov OI Scheffler, Brian/0000-0003-1968-8952 NR 21 TC 1 Z9 1 U1 1 U2 6 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 18 PY 2015 VL 3973 IS 3 BP 591 EP 600 PG 10 WC Zoology SC Zoology GA CK8AI UT WOS:000356459000012 PM 26249880 ER PT J AU de Oliveira, MLP Stover, E Thomson, JG AF de Oliveira, Maria Luiza Peixoto Stover, Ed Thomson, James G. TI The codA gene as a negative selection marker in Citrus SO SPRINGERPLUS LA English DT Article DE Citrus sinensis; codA gene; Cytosine deaminase; Negative selection marker; 5-Fluorocytosine ID CYTOSINE DEAMINASE GENE; SELECTABLE MARKER; TRANSFORMATION; ARABIDOPSIS; DNA; EXPRESSION AB The use of positive selectable marker genes is widespread in plant genetic transformation allowing transgenic cells to grow while repressing non-transgenic cells. Negative selectable markers, on the contrary, allow the repression or ablation of transgenic cells. The codA gene of Escherichia coli encodes cytosine deaminase that hydrolyzes 5-fluorocytosine (5-FC) into the cytotoxic compound 5 fluorouracil. We tested the transgenic expression of the bacterial codA gene in citrus as a conditional negative selection marker, with the goal of selecting against plant tissues in which a transgenic cassette has not been successfully removed. We developed transgenic citrus lines containing the selection cassette, codA:: nptll, driven by double enhanced CaMV35S promoter, verified by Southern blot analysis, RT-PCR, DsRed expression and subjected these transgenic lines to a 5-FC sensitivity assay. We found that, while non-transgenic citrus were unaffected by the presence of 5-FC, all of the transformed lines displayed symptoms of toxicity, indicating that the codA gene could be used as a negative selectable marker in Citrus, for post-transformation detection of the removal of undesired sequences. C1 [de Oliveira, Maria Luiza Peixoto; Stover, Ed] USDA ARS, Subtrop Insects & Hort Res Unit, Ft Pierce, FL 94710 USA. [Thomson, James G.] USDA ARS, Crop Improvement & Utilizat Res Unit, Albany, CA 94710 USA. RP Thomson, JG (reprint author), USDA ARS, Crop Improvement & Utilizat Res Unit, Albany, CA 94710 USA. EM James.Thomson@ars.usda.gov NR 18 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 2193-1801 J9 SPRINGERPLUS JI SpringerPlus PD JUN 17 PY 2015 VL 4 AR 264 DI 10.1186/s40064-015-1047-y PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CO6IT UT WOS:000359261100005 PM 26090311 ER PT J AU Renukaradhya, GJ Meng, XJ Calvert, JG Roof, M Lager, KM AF Renukaradhya, Gourapura J. Meng, Xiang-Jin Calvert, Jay G. Roof, Michael Lager, Kelly M. TI Inactivated and subunit vaccines against porcine reproductive and respiratory syndrome: Current status and future direction SO VACCINE LA English DT Review DE Porcine reproductive and respiratory; syndrome virus (PRRSV); Inactivated/killed PRRSV; Immunity; Cross-protection; Delivery system ID SYNDROME VIRUS PRRSV; MEDIATED IMMUNE-RESPONSES; NEUTRALIZING ANTIBODIES; IN-VIVO; EXPRESSING GP5; DNA VACCINE; NONSTRUCTURAL PROTEINS; NANOPARTICLE VACCINES; PROTECTIVE IMMUNITY; DELIVERY-SYSTEMS AB Within a few years of its emergence in the late 1980s, the PRRS virus had spread globally to become the foremost infectious disease concern for the pork industry. Since 1994, modified live-attenuated vaccines against porcine reproductive and respiratory syndrome virus (PRRSV-MLV) have been widely used, but have failed to provide complete protection against emerging and heterologous field strains of the virus. Moreover, like many other MLVs, PRRSV-MLVs have safety concerns including vertical and horizontal transmission of the vaccine virus and several documented incidences of reversion to virulence. Thus, the development of efficacious inactivated vaccines is warranted for the control and eradication of PRRS. Since the early 1990s, researchers have been attempting to develop inactivated PRRSV vaccines, but most of the candidates have failed to elicit protective immunity even against homologous virus challenge. Recent research findings relating to both inactivated and subunit candidate PRRSV vaccines have shown promise, but they need to be pursued further to improve their heterologous efficacy and cost-effectiveness before considering commercialization. In this comprehensive review, we provide information on attempts to develop PRRSV inactivated and subunit vaccines. These includes various virus inactivation strategies, adjuvants, nanoparticle-based vaccine delivery systems, DNA vaccines, and recombinant subunit vaccines produced using baculovirus, plant, and replication-deficient viruses as vector vaccines. Finally, future directions for the development of innovative non-infectious PRRSV vaccines are suggested. Undoubtedly there remains a need for novel PRRSV vaccine strategies targeted to deliver cross-protective, non-infectious vaccines for the control and eradication of PRRS. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Renukaradhya, Gourapura J.] Ohio State Univ, FAHRP, OARDC, Dept Vet Prevent Med, Wooster, OH 44691 USA. [Meng, Xiang-Jin] Virginia Polytech Inst & State Univ, Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. [Calvert, Jay G.] Zoetis, Kalamazoo, MI USA. [Roof, Michael] Boehringer Ingelheim Vetmed Inc, Ames, IA USA. [Lager, Kelly M.] USDA, Virol Swine Res Unit, Natl Anim Dis Ctr, Ames, IA USA. RP Renukaradhya, GJ (reprint author), Ohio State Univ, FAHRP, OARDC, 1680 Madison Ave, Wooster, OH 44691 USA. EM gourapura.1@osu.edu; kelly.lager@ars.usda.gov OI Gourapura, Renukaradhya/0000-0003-2861-180X FU NPB [13-246B] FX This is a commissioned literature review by the National Pork Board (NPB). The authors wish to thank the NPB (NPB Project #13-246B) for financial support. NR 88 TC 14 Z9 17 U1 12 U2 35 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUN 17 PY 2015 VL 33 IS 27 BP 3065 EP 3072 DI 10.1016/j.vaccine.2015.04.102 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA CL8PB UT WOS:000357235500001 PM 25980425 ER PT J AU Ma, LJ Qiao, JX Kong, XY Zou, YP Xu, XM Chen, XM Hu, XP AF Ma, Lijie Qiao, Jiaxing Kong, Xinyu Zou, Yiping Xu, Xiangming Chen, Xianming Hu, Xiaoping TI Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp tritici under Controlled Conditions SO PLOS ONE LA English DT Article ID PREDICTING STRIPE RUST; PACIFIC NORTHWEST; UNITED-STATES; EPIDEMIOLOGY; INFECTION; PATHOGEN; PCR; EXPRESSION; SELECTION; GENES AB Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0 degrees C and -5 degrees C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20 degrees C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring. C1 [Ma, Lijie; Qiao, Jiaxing; Kong, Xinyu; Zou, Yiping; Hu, Xiaoping] Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China. [Xu, Xiangming] East Malling Res, East Malling ME19 6BJ, Kent, England. [Chen, Xianming] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Hu, XP (reprint author), Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Taicheng Rd 3, Yangling 712100, Peoples R China. EM xphu@nwsuaf.edu.cn OI Xu, Xiangming/0000-0002-4567-7117 FU National Key Basic Research Program of China [2013CB127700]; National Natural Science Foundation of China [31071640, 31271985]; 111 Project from Education Ministry of China [B07049] FX This work was supported by the National Key Basic Research Program of China (2013CB127700), and the National Natural Science Foundation of China (31071640 and 31271985), and partially supported by the 111 Project from Education Ministry of China (B07049). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 2 Z9 2 U1 2 U2 24 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 17 PY 2015 VL 10 IS 6 AR e0130691 DI 10.1371/journal.pone.0130691 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK9NS UT WOS:000356567400154 PM 26083371 ER PT J AU Rancour, DM Hatfield, RD Marita, JM Rohr, NA Schmitz, RJ AF Rancour, David M. Hatfield, Ronald D. Marita, Jane M. Rohr, Nicholas A. Schmitz, Robert J. TI Cell wall composition and digestibility alterations in Brachypodium distachyon achieved through reduced expression of the UDP-arabinopyranose mutase SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE plant cell wall; biomass; Brachypodium distachyon; grass; nucleotide sugar; hydroxycinnamates ID AGROBACTERIUM-MEDIATED TRANSFORMATION; TANDEM MASS-SPECTROMETRY; DOWN-REGULATION; CROSS-LINKING; GENE FAMILY; HYDROXYCINNAMOYL TRANSFERASE; GLUCOSE DEHYDROGENASE; LIQUID-CHROMATOGRAPHY; ENZYMATIC DEGRADATION; CELLULOSE SYNTHESIS AB Nucleotide-activated sugars are essential substrates for plant cell-wall carbohydratepolymer biosynthesis. The most prevalent grass cell wall (CW) sugars are glucose (Glc), xylose (Xyl), and arabinose (Ara). These sugars are biosynthetically related via the UDP sugar interconversion pathway. We sought to target and generate UDP sugar interconversion pathway transgenic Brachypodium distachyon lines resulting in CW carbohydrate composition changes with improved digestibility and normal plant stature. Both RNAi-mediated gene-suppression and constitutive gene-expression approaches were performed. CWs from 336 To transgenic plants with normal appearance were screened for complete carbohydrate composition. RNAi mutants of BdiRGP1, a UDP-arabinopyranose mutase, resulted in large alterations in CW carbohydrate composition with significant decreases in CW Ara content but with minimal change in plant stature. Five independent RNAI-RGP1 T-1 plant lines were used for in-depth analysis of plant CWs. Real-time PCR analysis indicated that gene expression levels for BdiRGP1, BdRGP2, and BdRGP3 were reduced in RNAi-RGP1 plants to 15-20% of controls. CW Ara content was reduced by 23-51% of control levels. No alterations in CW Xyl and Glc content were observed. Corresponding decreases in CW ferulic acid (FA) and ferulic acid-dimers (FA-dimers) were observed. Additionally, CW p-coumarates (pCA) were decreased. We demonstrate the CW pCA decrease corresponds to Ara-coupled pCA. Xylanase-mediated digestibility of RNAi-RGP1 Brachypodium CWs resulted in a near twofold increase of released total carbohydrate. However, cellulolytic hydrolysis of CW material was inhibited in leaves of RNAI-FiGP1 mutants. Our results indicate that targeted manipulation of UDP sugar biosynthesis can result in biomass with substantially altered compositions and highlights the complex effect CW composition has on digestibility. C1 [Rancour, David M.; Hatfield, Ronald D.; Marita, Jane M.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Rohr, Nicholas A.; Schmitz, Robert J.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. RP Hatfield, RD (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM ronald.hatfield@ars.usda.gov FU University of Georgia FX The authors would like to thank Justin Marita and Emily Phillips for their assistance in harvesting plant tissues used for RNA-seq analysis. RNA-seq analysis was partially funded by start up funds from the University of Georgia to RS. The authors would like to thank Nick Santoro and the Great Lakes Bioenergy Research Center, CW Digestibility Platform at Michigan State University for performing the Accelerase 1000 enzyme digestibility analysis on our CW samples. NR 100 TC 4 Z9 4 U1 2 U2 25 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 17 PY 2015 VL 6 AR 446 DI 10.3389/fpls.2015.00446 PG 20 WC Plant Sciences SC Plant Sciences GA CL9HV UT WOS:000357289300001 PM 26136761 ER PT J AU Hotchkiss, AT Nunez, A Strahan, GD Chau, HK White, AK Marais, JPJ Hom, K Vakkalanka, MS Di, R Yam, KL Khoo, C AF Hotchkiss, Arland T., Jr. Nunez, Alberto Strahan, Gary D. Chau, Hoa K. White, Andre K. Marais, Jannie P. J. Hom, Kellie Vakkalanka, Malathi S. Di, Rong Yam, Kit L. Khoo, Christina TI Cranberry Xyloglucan Structure and Inhibition of Escherichia coli Adhesion to Epithelial Cells SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE cranberry; arabino-xyloglucan; Escherichia coli; anti-adhesive; Urinary tract infection ID VACCINIUM-MACROCARPON; BLUEBERRY JUICES; IN-VITRO; OLIGOSACCHARIDES; ADHERENCE; POLYSACCHARIDES; SPECTROSCOPY; PECTIN; PLANTS; WALLS AB Cranberry juice has been recognized as a treatment for urinary tract infections on the basis of scientific reports of proanthocyanidin anti-adhesion activity against Escherichia coli as well as from folklore. Xyloglucan oligosaccharides were detected in cranberry juice and the residue remaining following commercial juice extraction that included pectinase maceration of the pulp. A novel xyloglucan Was detected through tandem mass spectrometry analysis of an ion at m/z 1055 that was determined to be a branched, three hexose, four pentose oligosaccharide consistent with an arabino-xyloglucan structure. Two-dimensional nuclear magnetic resonance spectroscopy analysis provided through-bond correlations for the alpha-L-Araf (1 -> 2) alpha-D-Xylp (1 -> 6) beta-D-Glcp sequence, proving the S-type cranberry xyloglucan structure. Cranberry xyloglucan-rich fractions inhibited the adhesion of E coli CFT073 and UT189 strains to T24 human bladder epithelial cells and that of E. coli O157:H7 to HT29 human colonic epithelial cells. SSGG xyloglucan oligosaccharides represent a new cranberry bioactive component with E coli anti-adhesion activity and high affinity for type, 1 fimbriae. C1 [Hotchkiss, Arland T., Jr.; Nunez, Alberto; Strahan, Gary D.; Chau, Hoa K.; White, Andre K.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Marais, Jannie P. J.; Khoo, Christina] Ocean Spray Cranberries Inc, Lakeville Middleboro, MA 02349 USA. [Hom, Kellie] Univ Maryland, Sch Pharm, Dept Pharmaceut Sci, Baltimore, MD 21201 USA. [Vakkalanka, Malathi S.; Yam, Kit L.] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA. [Di, Rong] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. RP Hotchkiss, AT (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM arland.hotchkiss@ars.usda.gov FU Cooperative Research and Development Agreement; Ocean Spray Cranberries, Inc. [58-3K95-1-1503] FX This work was supported by a Cooperative Research and Development Agreement with Ocean Spray Cranberries, Inc. (58-3K95-1-1503). Mention of brand or firm name does not constitute an endorsement of the U.S. Department of Agriculture above others of a similar nature not mentioned. NR 38 TC 6 Z9 6 U1 1 U2 26 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 17 PY 2015 VL 63 IS 23 BP 5622 EP 5633 DI 10.1021/acs.jafc.5b00730 PG 12 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CL2DO UT WOS:000356754000007 PM 25973733 ER PT J AU Ruan, YY Konstantinov, AS Prathapan, KD Ge, SQ Yang, XK AF Ruan, Yong-Ying Konstantinov, Alexander S. Prathapan, K. D. Ge, Si-Qin Yang, Xing-Ke TI Penghou, a new genus of flea beetles from China (Coleoptera: Chrysomelidae: Galerucinae: Alticini) SO ZOOTAXA LA English DT Article DE new genus; new species; flea beetle; Alticinae; China; Yunnan AB A new genus (Penghou) with a single new species (P. yulongshan) from Yunnan Province in China is described and illustrated. It is compared to Hespera Weise, Hesperomorpha Ogloblin, Laotzeus Chen, Luperomorpha Weise, Mandarella Duvivier, Omeiana Chen, Stenoluperus Ogloblin and Taiwanohespera Kimoto. C1 [Ruan, Yong-Ying; Ge, Si-Qin; Yang, Xing-Ke] Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China. [Konstantinov, Alexander S.] USDA ARS, Systemat Entomol Lab, Washington, DC 20250 USA. [Ruan, Yong-Ying] Univ Chinese Acad Sci, Beijing 100039, Peoples R China. [Prathapan, K. D.] Kerala Agr Univ, Dept Entomol, Trivandrum 695522, Kerala, India. RP Konstantinov, AS (reprint author), USDA ARS, Systemat Entomol Lab, Washington, DC 20250 USA. EM alex.konstantinov@ars.usda.gov; yangxk@ioz.ac.cn FU National Science Foundation of China [31372239]; National Science Fund for Fostering Talents in Basic Research (Special Subjects in Animal Taxonomy) [NSFC-J1210002] FX We are grateful to M. Volkovitsh (Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia) for his friendship and camaraderie during collecting trip in China in 2002. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer. This research was supported by grants from the National Science Foundation of China to Xingke Yang (PI, Grant No. 31372239) and the National Science Fund for Fostering Talents in Basic Research (Special Subjects in Animal Taxonomy, NSFC-J1210002). NR 7 TC 0 Z9 0 U1 1 U2 10 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 17 PY 2015 VL 3973 IS 2 BP 300 EP 308 PG 9 WC Zoology SC Zoology GA CK8AA UT WOS:000356458100005 PM 26249860 ER PT J AU Chen, CYO Holbrook, M Duess, MA Dohadwala, MM Hamburg, NM Asztalos, BF Milbury, PE Blumberg, JB Vita, JA AF Chen, C-Y. Oliver Holbrook, Monika Duess, Mai-Ann Dohadwala, Mustali M. Hamburg, Naomi M. Asztalos, Bela F. Milbury, Paul E. Blumberg, Jeffrey B. Vita, Joseph A. TI Effect of almond consumption on vascular function in patients with coronary artery disease: a randomized, controlled, cross-over trial SO NUTRITION JOURNAL LA English DT Article DE Almonds; Coronary arterial disease; Dietary quality; Endothelial function; Inflammation; Oxidative stress ID DENSITY-LIPOPROTEIN-CHOLESTEROL; TYPE-2 DIABETES-MELLITUS; C-REACTIVE PROTEIN; HEART-DISEASE; CARDIOVASCULAR-DISEASE; RISK-FACTORS; HYPERCHOLESTEROLEMIC SUBJECTS; ENDOTHELIAL FUNCTION; MEDITERRANEAN DIET; OXIDATIVE STRESS AB Objective: Almonds reduce cardiovascular disease risk via cholesterol reduction, anti-inflammation, glucoregulation, and antioxidation. The objective of this randomized, controlled, cross-over trial was to determine whether the addition of 85 g almonds daily to a National Cholesterol Education Program (NCEP) Step 1 diet (ALM) for 6 weeks would improve vascular function and inflammation in patients with coronary artery disease (CAD). Research design and methods: A randomized, controlled, crossover trial was conducted in Boston, MA to test whether as compared to a control NCEP Step 1 diet absent nuts (CON), incorporation of almonds (85 g/day) into the CON diet (ALM) would improve vascular function and inflammation. The study duration was 22 weeks including a 6-weeks run-in period, two 6-weeks intervention phases, and a 4-weeks washout period between the intervention phases. A total of 45 CAD patients (27 F/18 M, 45-77 y, BMI = 20-41 kg/m(2)) completed the study. Drug therapies used by patients were stable throughout the duration of the trial. Results: The addition of almonds to the CON diet increased plasma a-tocopherol status by a mean of 5.8 %, reflecting patient compliance (P <= 0.05). However, the ALM diet did not alter vascular function assessed by measures of flow-mediated dilation, peripheral arterial tonometry, and pulse wave velocity. Further, the ALM diet did not significantly modify the serum lipid profile, blood pressure, C-reactive protein, tumor necrosis factor-a or E-selectin. The ALM diet tended to decrease vascular cell adhesion molecule-1 by 5.3 % (P = 0.064) and increase urinary nitric oxide by 17.5 % (P = 0.112). The ALM intervention improved the overall quality of the diet by increasing calcium, magnesium, choline, and fiber intakes above the Estimated Average Requirement (EAR) or Recommended Dietary Allowance (RDA). Conclusions: Thus, the addition of almonds to a NECP Step 1 diet did not significantly impact vascular function, lipid profile or systematic inflammation in CAD patients receiving good medical care and polypharmacy therapies but did improve diet quality without any untoward effect. C1 [Chen, C-Y. Oliver; Asztalos, Bela F.; Milbury, Paul E.; Blumberg, Jeffrey B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Holbrook, Monika; Duess, Mai-Ann; Dohadwala, Mustali M.; Hamburg, Naomi M.; Vita, Joseph A.] Boston Univ, Evans Dept Med, Sch Med, Boston, MA 02215 USA. [Holbrook, Monika; Duess, Mai-Ann; Dohadwala, Mustali M.; Hamburg, Naomi M.; Vita, Joseph A.] Boston Univ, Whitaker Cardiovasc Inst, Sch Med, Boston, MA 02215 USA. [Chen, C-Y. Oliver] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Chen, CYO (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM oliver.chen@tufts.edu FU USDA Agricultural Research Service [58-1950-014]; Almond Board of California FX The study was funded by the USDA Agricultural Research Service under Cooperative Agreement No. 58-1950-014 and the Almond Board of California. None of the authors declare a conflict of interest. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 47 TC 1 Z9 1 U1 10 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1475-2891 J9 NUTR J JI Nutr. J. PD JUN 17 PY 2015 VL 14 AR 61 DI 10.1186/s12937-015-0049-5 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CK7NE UT WOS:000356418400001 PM 26080804 ER PT J AU Fernandez-Friera, L Penalvo, JL Fernandez-Ortiz, A Ibanez, B Lopez-Melgar, B Laclaustra, M Oliva, B Mocoroa, A Mendiguren, J de Vega, VM Garcia, L Molina, J Sanchez-Gonzalez, J Guzman, G Alonso-Farto, JC Guallar, E Civeira, F Sillesen, H Pocock, S Ordovas, JM Sanz, G Jimenez-Borreguero, LJ Fuster, V AF Fernandez-Friera, Leticia Penalvo, Jose L. Fernandez-Ortiz, Antonio Ibanez, Borja Lopez-Melgar, Beatriz Laclaustra, Martin Oliva, Belen Mocoroa, Agustin Mendiguren, Jose Martinez de Vega, Vicente Garcia, Laura Molina, Jesus Sanchez-Gonzalez, Javier Guzman, Gabriela Alonso-Farto, Juan C. Guallar, Eliseo Civeira, Fernando Sillesen, Henrik Pocock, Stuart Ordovas, Jose M. Sanz, Gines Jesus Jimenez-Borreguero, Luis Fuster, Valentin TI Prevalence, Vascular Distribution, and Multiterritorial Extent of Subclinical Atherosclerosis in a Middle-Aged Cohort The PESA (Progression of Early Subclinical Atherosclerosis) Study SO CIRCULATION LA English DT Article DE atherosclerosis; epidemiology; multidetector computed tomography; population; risk assessment; ultrasonography ID CORONARY-HEART-DISEASE; CARDIOVASCULAR-DISEASE; ARTERY CALCIUM; YOUNG-ADULTS; RISK; EVENTS; ASSOCIATION; DESIGN; HEALTH; PLAQUE AB Background-Data are limited on the presence, distribution, and extent of subclinical atherosclerosis in middle-aged populations. Methods and Results-The PESA (Progression of Early Subclinical Atherosclerosis) study prospectively enrolled 4184 asymptomatic participants 40 to 54 years of age (mean age, 45.8 years; 63% male) to evaluate the systemic extent of atherosclerosis in the carotid, abdominal aortic, and iliofemoral territories by 2-/3-dimensional ultrasound and coronary artery calcification by computed tomography. The extent of subclinical atherosclerosis, defined as presence of plaque or coronary artery calcification >= 1, was classified as focal (1 site affected), intermediate (2-3 sites), or generalized (4-6 sites) after exploration of each vascular site (right/left carotids, aorta, right/left iliofemorals, and coronary arteries). Subclinical atherosclerosis was present in 63% of participants (71% of men, 48% of women). Intermediate and generalized atherosclerosis was identified in 41%. Plaques were most common in the iliofemorals (44%), followed by the carotids (31%) and aorta (25%), whereas coronary artery calcification was present in 18%. Among participants with low Framingham Heart Study (FHS) 10-year risk, subclinical disease was detected in 58%, with intermediate or generalized disease in 36%. When longer-term risk was assessed (30-year FHS), 83% of participants at high risk had atherosclerosis, with 66% classified as intermediate or generalized. Conclusions-Subclinical atherosclerosis was highly prevalent in this middle-aged cohort, with nearly half of the participants classified as having intermediate or generalized disease. Most participants at high FHS risk had subclinical disease; however, extensive atherosclerosis was also present in a substantial number of low-risk individuals, suggesting added value of imaging for diagnosis and prevention. C1 [Fernandez-Friera, Leticia; Penalvo, Jose L.; Fernandez-Ortiz, Antonio; Ibanez, Borja; Lopez-Melgar, Beatriz; Laclaustra, Martin; Oliva, Belen; Garcia, Laura; Molina, Jesus; Guzman, Gabriela; Sillesen, Henrik; Pocock, Stuart; Ordovas, Jose M.; Sanz, Gines; Jesus Jimenez-Borreguero, Luis; Fuster, Valentin] Ctr Nacl Invest Cardiovasc Carlos III, Madrid, Spain. [Fernandez-Friera, Leticia; Lopez-Melgar, Beatriz] Hosp Univ Monteprincipe, Madrid, Spain. [Laclaustra, Martin] Autonomous Univ Madrid, Madrid, Spain. [Laclaustra, Martin] St Louis Univ, St Louis, MO 63103 USA. [Fernandez-Ortiz, Antonio; Ibanez, Borja] Univ Complutense Madrid, Hosp Clin San Carlos, Madrid, Spain. [Mocoroa, Agustin; Mendiguren, Jose] Banco Santander, Madrid, Spain. [Martinez de Vega, Vicente] Hosp Univ Quiron, Madrid, Spain. [Sanchez-Gonzalez, Javier] Philips Healthcare, Madrid, Spain. [Guzman, Gabriela] Hosp Univ La Paz, Madrid, Spain. [Alonso-Farto, Juan C.] Hosp Gen Univ Gregorio Maranon, Madrid, Spain. [Guallar, Eliseo] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Civeira, Fernando] Hosp Univ Miguel Servet, Inst Aragones Ciencias Salud, Lipids Unit, Zaragoza, Spain. [Civeira, Fernando] Hosp Univ Miguel Servet, Inst Aragones Ciencias Salud, Mol Res Lab, Zaragoza, Spain. [Sillesen, Henrik] Univ Copenhagen, Rigshosp, DK-1168 Copenhagen, Denmark. [Pocock, Stuart] London Sch Hyg & Trop Med, London, England. [Ordovas, Jose M.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jesus Jimenez-Borreguero, Luis] Hosp Univ La Princesa, Madrid, Spain. [Fuster, Valentin] Mt Sinai Sch Med, New York, NY USA. RP Fuster, V (reprint author), Icahn Sch Med Mt Sinai, One Gustave L Levy Pl,Box 1030, New York, NY 10029 USA. EM valentin.fuster@mountsinai.org RI Ibanez, Borja/J-6993-2014; Fuster, Valentin/H-4319-2015; Fernandez-Ortiz, Antonio/B-2227-2017; Fernandez Friera, Leticia/E-9931-2015; OI Ibanez, Borja/0000-0002-5036-254X; Fuster, Valentin/0000-0002-9043-9986; Fernandez-Ortiz, Antonio/0000-0002-3239-1910; Fernandez Friera, Leticia/0000-0002-4237-2166; Jimenez-Borreguero, Luis Jesus/0000-0001-5870-237X FU CNIC; Fundacion Botin of Banco Santander; Spanish Ministry of Economy and Competitiveness; Pro-CNIC Foundation FX This study was supported by a noncompetitive unrestricted grant shared equally between the CNIC and the Fundacion Botin of Banco Santander. The PESA study is a noncommercial study that is independent of the healthcare and pharmaceutical industry. The CNIC is supported by the Spanish Ministry of Economy and Competitiveness and the Pro-CNIC Foundation. NR 34 TC 39 Z9 39 U1 4 U2 9 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0009-7322 EI 1524-4539 J9 CIRCULATION JI Circulation PD JUN 16 PY 2015 VL 131 IS 24 BP 2104 EP 2113 DI 10.1161/CIRCULATIONAHA.114.014310 PG 10 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA DC5IK UT WOS:000369254500009 PM 25882487 ER PT J AU Ducken, DR Brown, WC Alperin, DC Brayton, KA Reif, KE Turse, JE Palmer, GH Noh, SM AF Ducken, Deirdre R. Brown, Wendy C. Alperin, Debra C. Brayton, Kelly A. Reif, Kathryn E. Turse, Joshua E. Palmer, Guy H. Noh, Susan M. TI Subdominant Outer Membrane Antigens in Anaplasma marginale: Conservation, Antigenicity, and Protective Capacity Using Recombinant Protein SO PLOS ONE LA English DT Article ID MAMMALIAN HOST-CELLS; MAJOR SURFACE PROTEIN-2; BOOPHILUS-MICROPLUS; VACCINE DEVELOPMENT; IMMUNOGENICITY; IMMUNITY; IDENTIFICATION; INFECTION; SUPERINFECTION; TRANSMISSION AB Anaplasma marginale is a tick-borne rickettsial pathogen of cattle with a worldwide distribution. Currently a safe and efficacious vaccine is unavailable. Outer membrane protein (OMP) extracts or a defined surface protein complex reproducibly induce protective immunity. However, there are several knowledge gaps limiting progress in vaccine development. First, are these OMPs conserved among the diversity of A. marginale strains circulating in endemic regions? Second, are the most highly conserved outer membrane proteins in the immunogens recognized by immunized and protected animals? Lastly, can this subset of OMPs recognized by antibody from protected vaccinates and conserved among strains recapitulate the protection of outer membrane vaccines? To address the first goal, genes encoding OMPs AM202, AM368, AM854, AM936, AM1041, and AM1096, major subdominant components of the outer membrane, were cloned and sequenced from geographically diverse strains and isolates. AM202, AM936, AM854, and AM1096 share 99.9 to 100% amino acid identity. AM1041 has 97.1 to 100% and AM368 has 98.3 to 99.9% amino acid identity. While all four of the most highly conserved OMPs were recognized by IgG from animals immunized with outer membranes, linked surface protein complexes, or unlinked surface protein complexes and shown to be protected from challenge, the highest titers and consistent recognition among vaccinates were to AM854 and AM936. Consequently, animals were immunized with recombinant AM854 and AM936 and challenged. Recombinant vaccinates and purified outer membrane vaccinates had similar IgG and IgG2 responses to both proteins. However, the recombinant vaccinates developed higher bacteremia after challenge as compared to adjuvant-only controls and outer membrane vaccinates. These results provide the first evidence that vaccination with specific antigens may exacerbate disease. Progressing from the protective capacity of outer membrane formulations to recombinant vaccines requires testing of additional antigens, optimization of the vaccine formulation and a better understanding of the protective immune response. C1 [Ducken, Deirdre R.; Noh, Susan M.] USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [Ducken, Deirdre R.; Brown, Wendy C.; Alperin, Debra C.; Brayton, Kelly A.; Reif, Kathryn E.; Turse, Joshua E.; Noh, Susan M.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Brayton, Kelly A.; Noh, Susan M.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. RP Noh, SM (reprint author), USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. EM snoh@vetmed.wsu.edu FU United States Department of Agriculture-Agricultural Research Service [5348-32000-033-00D, 5348-3200-033-03S]; National Institutes of Health [R37 AI44005] FX This work was supported by the United States Department of Agriculture-Agricultural Research Service grant 5348-32000-033-00D, United States Department of Agriculture-Agricultural Research Service cooperative agreement 5348-3200-033-03S, and National Institutes of Health R37 AI44005. NR 48 TC 5 Z9 5 U1 0 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 16 PY 2015 VL 10 IS 6 AR e0129309 DI 10.1371/journal.pone.0129309 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK9EB UT WOS:000356542300021 PM 26079491 ER PT J AU Guo, SG Sun, HH Zhang, HY Liu, JA Ren, Y Gong, GY Jiao, C Zheng, Y Yang, WC Fei, ZJ Xu, Y AF Guo, Shaogui Sun, Honghe Zhang, Haiying Liu, Jingan Ren, Yi Gong, Guoyi Jiao, Chen Zheng, Yi Yang, Wencai Fei, Zhangjun Xu, Yong TI Comparative Transcriptome Analysis of Cultivated and Wild Watermelon during Fruit Development SO PLOS ONE LA English DT Article ID ALKALINE ALPHA-GALACTOSIDASE; WALL DISASSEMBLY EVENTS; CUCUMIS-MELO L; CITRULLUS-LANATUS; EXPRESSION ANALYSIS; RUBUS-IDAEUS; RNA-SEQ; ETHYLENE; TOMATO; METABOLISM AB Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an important vegetable crop world-wide. Watermelon fruit quality is a complex trait determined by various factors such as sugar content, flesh color and flesh texture. Fruit quality and developmental process of cultivated and wild watermelon are highly different. To systematically understand the molecular basis of these differences, we compared transcriptome profiles of fruit tissues of cultivated watermelon 97103 and wild watermelon PI296341-FR. We identified 2,452, 826 and 322 differentially expressed genes in cultivated flesh, cultivated mesocarp and wild flesh, respectively, during fruit development. Gene ontology enrichment analysis of these genes indicated that biological processes and metabolic pathways related to fruit quality such as sweetness and flavor were significantly changed only in the flesh of 97103 during fruit development, while those related to abiotic stress response were changed mainly in the flesh of PI296341-FR. Our comparative transcriptome profiling analysis identified critical genes potentially involved in controlling fruit quality traits including alpha-galactosidase, invertase, UDP-galactose/glucose pyrophosphorylase and sugar transporter genes involved in the determination of fruit sugar content, phytoene synthase, beta-carotene hydroxylase, 9-cis-epoxycarotenoid dioxygenase and carotenoid cleavage dioxygenase genes involved in carotenoid metabolism, and 4-coumarate: coenzyme A ligase, cellulose synthase, pectinesterase, pectinesterase inhibitor, polygalacturonase inhibitor and alpha-mannosidase genes involved in the regulation of flesh texture. In addition, we found that genes in the ethylene biosynthesis and signaling pathway including ACC oxidase, ethylene receptor and ethylene responsive factor showed highly ripening-associated expression patterns, indicating a possible role of ethylene in fruit development and ripening of watermelon, a non-climacteric fruit. Our analysis provides novel insights into watermelon fruit quality and ripening biology. Furthermore, the comparative expression profile data we developed provides a valuable resource to accelerate functional studies in watermelon and facilitate watermelon crop improvement. C1 [Guo, Shaogui; Yang, Wencai] China Agr Univ, Coll Agron & Biotechnol, Dept Vegetable Sci, Beijing Key Lab Growth & Dev Regulat Protected Ve, Beijing 100094, Peoples R China. [Guo, Shaogui; Sun, Honghe; Zhang, Haiying; Liu, Jingan; Ren, Yi; Gong, Guoyi; Xu, Yong] Natl Engn Res Ctr Vegetables, Key Lab Biol & Genet Improvement Hort Crops North, Beijing, Peoples R China. [Sun, Honghe; Jiao, Chen; Zheng, Yi; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Fei, ZJ (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM zf25@cornell.edu; xuyong@nercv.org RI Yang, Wencai/G-6517-2011; Zheng, Yi/F-6150-2016 OI Zheng, Yi/0000-0002-8042-7770 FU Ministry of Science and Technology of the People's Republic of China [2012AA100103, 2012AA100101, 2012AA100105, 2012BAD02B03, 2013BAD01B04-13, 2012BAD50G01, 2011BAD35B07]; National Natural Science Foundation of China [31272184, 31171980, 31401893, 31301783, 31361140355]; Beijing Natural Science Foundation [6141001, 6144023]; Ministry of Agriculture of the People's Republic of China [CARS-26]; Beijing Municipal Science and Technology Commission of China [Z121105002512037, Z131100003113012]; Beijing Excellent Talents Programme Foundation [2014000021223TD03, 2013D002020000003] FX This work was funded by the Ministry of Science and Technology of the People's Republic of China (2012AA100103, 2012AA100101, 2012AA100105, 2012BAD02B03, 2013BAD01B04-13, 2012BAD50G01 and 2011BAD35B07), National Natural Science Foundation of China (31272184, 31171980, 31401893, 31301783, 31361140355), Beijing Natural Science Foundation (6141001, 6144023), the Ministry of Agriculture of the People's Republic of China (CARS-26), the Beijing Municipal Science and Technology Commission of China (Z121105002512037, Z131100003113012) and the Beijing Excellent Talents Programme Foundation (2014000021223TD03, 2013D002020000003) to YX, HZ and SG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 62 TC 5 Z9 6 U1 12 U2 67 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 16 PY 2015 VL 10 IS 6 AR e0130267 DI 10.1371/journal.pone.0130267 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK9EB UT WOS:000356542300041 PM 26079257 ER PT J AU He, ZQ Zhang, HL Olk, DC AF He, Zhongqi Zhang, Hailin Olk, Dan C. TI Chemical Composition of Defatted Cottonseed and Soy Meal Products SO PLOS ONE LA English DT Article ID ADHESIVE PROPERTIES; PROTEIN ADHESIVES; AMINO-COMPOUNDS; SOILS; DIGESTIBILITY; MANAGEMENT; FRACTIONS; IMPROVE; MAPLE; WATER AB Chemical composition is critical information for product quality and exploration of new use. Hence defatted cottonseed meals from both glanded (with gossypol) and glandless (without gossypol) cotton seeds were separated into water soluble and insoluble fractions, or water soluble, alkali soluble as well as total protein isolates. The contents of gossypol, total protein and amino acids, fiber and carbohydrates, and selected macro and trace elements in these products were determined and compared with each other and with those of soy meal products. Data reported in this work improved our understanding on the chemical composition of different cottonseed meal products that is helpful for more economical utilization of these products. These data would also provide a basic reference for product standards and quality control when the production of the cottonseed meal products comes to pilot and industrial scales. C1 [He, Zhongqi] USDA ARS, So Reg Res Ctr, New Orleans, LA 70122 USA. [Zhang, Hailin] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Olk, Dan C.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. RP He, ZQ (reprint author), USDA ARS, So Reg Res Ctr, New Orleans, LA 70122 USA. EM Zhongqi.He@ars.usda.gov NR 44 TC 4 Z9 4 U1 4 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 16 PY 2015 VL 10 IS 6 AR e0129933 DI 10.1371/journal.pone.0129933 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK9EB UT WOS:000356542300036 PM 26079931 ER PT J AU Schaefer, M Goldman, E Bartuska, AM Sutton-Grier, A Lubchenco, J AF Schaefer, Mark Goldman, Erica Bartuska, Ann M. Sutton-Grier, Ariana Lubchenco, Jane TI Nature as capital: Advancing and incorporating ecosystem services in United States federal policies and programs SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ecosystem services; natural capital; environmental policy; US federal agencies AB The concept of nature as capital is gaining visibility in policies and practices in both the public and private sectors. This change is due to an improved ability to assess and value ecosystem services, as well as to a growing recognition of the potential of an ecosystem services approach to make tradeoffs in decision making more transparent, inform efficient use of resources, enhance resilience and sustainability, and avoid unintended negative consequences of policy actions. Globally, governments, financial institutions, and corporations have begun to incorporate natural capital accounting in their policies and practices. In the United States, universities, nongovernmental organizations, and federal agencies are actively collaborating to develop and apply ecosystem services concepts to further national environmental and economic objectives. Numerous federal agencies have begun incorporating these concepts into land use planning, water resources management, and preparations for, and responses to, climate change. Going forward, well-defined policy direction will be necessary to institutionalize ecosystem services approaches in federal agencies, as well as to guide intersector and interdisciplinary collaborative research and development efforts. In addition, a new generation of decision support tools are needed to further the practical application of ecosystem services principles in policymaking and commercial activities. Improved performance metrics are needed, as are mechanisms to monitor the status of ecosystem services and assess the environmental and economic impacts of policies and programs. A greater national and international financial commitment to advancing ecosystem services and natural capital accounting would likely have broad, long-term economic and environmental benefits. C1 [Schaefer, Mark] Woodrow Wilson Int Ctr Scholars, Sci & Technol Innovat Program, Washington, DC 20004 USA. [Goldman, Erica] COMPASS, Silver Spring, MD 20910 USA. [Bartuska, Ann M.] USDA, Washington, DC 20250 USA. [Sutton-Grier, Ariana] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20740 USA. [Sutton-Grier, Ariana] NOAA, Natl Ocean Serv, Silver Spring, MD 20910 USA. [Lubchenco, Jane] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. RP Schaefer, M (reprint author), Woodrow Wilson Int Ctr Scholars, Sci & Technol Innovat Program, Washington, DC 20004 USA. EM markschaefer24@msn.com OI Sutton-Grier, Ariana/0000-0002-1242-7728 NR 28 TC 14 Z9 14 U1 9 U2 49 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 16 PY 2015 VL 112 IS 24 BP 7383 EP 7389 DI 10.1073/pnas.1420500112 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK5GS UT WOS:000356251800032 PM 26082544 ER PT J AU Eardley, C Griswold, T AF Eardley, Connal Griswold, Terry TI Taxonomic revision of Plesianthidium Cameron (Apoidea: Megachilidae: Anthidiini), an endemic southern African bee genus SO ZOOTAXA LA English DT Article DE Afrotropical; pollinator; pollen collector AB The southern African bee genus Plesianthidium Cameron is revised. It comprises twelve species. Two new species are described: Plesianthidium (Spinanthidium) richtersveldense sp. nov. (Namibia and South Africa) and P. (S.) namaquaense sp. nov. (South Africa). Two species, Plesianthidium calvini (Cockerell, 1932) and P. rufocaudatum (Friese, 1909), are reinstated as valid species. Keys for the identification of the species of Plesianthidium are given. C1 [Eardley, Connal] Agr Res Council, ZA-0121 Queenswood, South Africa. [Eardley, Connal] Univ KwaZulu Natal, Sch Biol & Conservat Sci, ZA-3209 Pietermaritzburg, South Africa. [Griswold, Terry] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. RP Eardley, C (reprint author), Agr Res Council, Private Bag X134, ZA-0121 Queenswood, South Africa. EM eardleyc@arc.agric.za; terry.grisold@ars.usda.gov NR 23 TC 1 Z9 1 U1 0 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 16 PY 2015 VL 3973 IS 1 BP 1 EP 56 PG 56 WC Zoology SC Zoology GA CK7ZU UT WOS:000356457500001 PM 26249712 ER PT J AU Reina, LD Perez-Diaz, IM Breidt, F Azcarate-Peril, MA Medina, E Butz, N AF Reina, L. D. Perez-Diaz, I. M. Breidt, F. Azcarate-Peril, M. A. Medina, E. Butz, N. TI Characterization of the microbial diversity in yacon spontaneous fermentation at 20 degrees C SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Fermented vegetables; Lactic acid bacteria; Heterolactic fermentation ID LACTIC-ACID BACTERIA; COMMUNITIES; MANNITOL; SEARCH AB The prebiotic fructooligosaccharide content of yacon makes this root an attractive alternative for the supplementation of a variety of food products. The preservation of yacon by fermentation has been proposed as an alternative to increase the probiotic content of the root concomitantly with its shelf life. Thus the fermented yacon could have significant functional content. The objective of this research was to characterize the biochemistry and microbiology of spontaneous yacon fermentation with 2% NaCl and define the viability of the proposed process. The biochemical analysis of spontaneous heterolactic fermentation of yacon showed a progressive drop in pH with increased lactic and acetic acids, and the production of mannitol during fermentation. The microbial ecology of yacon fermentation was investigated using culture-dependent and culture-independent methods. Bacterial cell counts revealed a dominance of lactic acid bacteria (LAB) over yeasts, which were also present during the first 2 days of the fermentation. Results showed that the heterofermentative LAB were primarily Leuconostoc species, thus it presents a viable method to achieve long term preservation of this root. Published by Elsevier B.V. C1 [Reina, L. D.; Medina, E.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Perez-Diaz, I. M.; Breidt, F.] USDA ARS, SAA, Food Sci Res Unit, Raleigh, NC 27695 USA. [Azcarate-Peril, M. A.; Butz, N.] Univ N Carolina, Sch Med, Dept Cell Biol & Physiol, Microbiome Core Facil, Chapel Hill, NC 27599 USA. [Medina, E.] CSIC, Inst Grasa, Dept Food Biotechnol, E-41080 Seville, Spain. RP Reina, LD (reprint author), N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. EM ldreina@yahoo.com RI Eduardo, Medina/D-3296-2014 OI Eduardo, Medina/0000-0002-9978-4524 FU National Institutes of Health [P30 DK34987]; Simmons award; Spanish Government (MECD) FX The author thanks Ms. Sandra Parker and Ms. Janet Hayes at the USDA-ARS, SAA Food Science Research Unit located in Raleigh, NC for the administrative support and technical assistance, respectively. We also thank Nevermore Farm in Arbuckle, CA, USA for supplying yacon. This research was partially supported (10%) by the National Institutes of Health grant number P30 DK34987, and a Simmons award. A Postdoctoral Fellowship from the Spanish Government (MECD) fully funded Dr. E. Medina. NR 25 TC 0 Z9 1 U1 1 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JUN 16 PY 2015 VL 203 BP 35 EP 40 DI 10.1016/j.ijfoodmicro.2015.03.007 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CI2PE UT WOS:000354588700006 PM 25777679 ER PT J AU Kane, SE Holler, LD Braun, LJ Neill, JD Young, DB Ridpath, JF Chase, CCL AF Kane, Susan E. Holler, Larry D. Braun, Lyle J. Neill, John D. Young, Douglas B. Ridpath, Julia F. Chase, Christopher C. L. TI Bovine viral diarrhea virus outbreak in a beef cow herd in South Dakota SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Article ID MUCOSAL DISEASE; BVDV-INFECTION; CATTLE; SASKATCHEWAN; PERFORMANCE; DIAGNOSIS AB Case Description-136 pregnant beef cows were purchased in the fall of 2003. The following spring, 128 cows calved as expected; 8 cows were believed to have aborted with the fetuses unavailable for evaluation. Of the 128 calves born, 8 died within 2 weeks after birth and 9 were born with congenital abnormalities. Clinical Findings-Cows and their calves were evaluated for bovine viral diarrhea virus (BVDV) infection. Forty-four of 120 calves, but 0 cows, tested positive for BVDV antigen by immunohistochemical staining of ear notch specimens. Treatment and Outcome-Five BVDV test-positive calves died shortly after weaning, and the remaining 39 BVDV test-positive calves were moved to an isolated feedlot and retested for BVDV at 5 to 6 months of age; 36 had positive results, which indicated that they were persistently infected (PI) with BVDV, whereas 3 had negative results, which indicated that they were transiently infected with BVDV at the time of the first test. All PI calves were infected with the same BVDV type 2a strain. As yearlings, 17 of the 36 PI calves died peracutely with lesions consistent with mucosal disease, 6 died without gross lesions, and 2 were euthanized because of chronic ill thrift. The remaining 11 PI calves appeared healthy and were sold for slaughter. Screening of the following year's calf crop for BVDV by use of immunohistochemical staining of ear-notch specimens yielded negative results for all calves. Clinical Relevance-Introduction of BVDV into a naive cow herd resulted in a loss of 44% of the calf crop subsequent to reproductive loss, poor thrift, and mucosal disease. C1 [Kane, Susan E.; Holler, Larry D.; Braun, Lyle J.; Chase, Christopher C. L.] S Dakota State Univ, Coll Agr & Biol Sci, Dept Vet & Biomed Sci, Brookings, SD 57006 USA. [Neill, John D.; Ridpath, Julia F.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Young, Douglas B.] S Dakota State Univ, State South Dakota Antelope Range & Livestock Res, Buffalo, SD 57720 USA. RP Chase, CCL (reprint author), S Dakota State Univ, Coll Agr & Biol Sci, Dept Vet & Biomed Sci, Brookings, SD 57006 USA. EM Christopher.Chase@sdstate.edu FU Boehringer Ingelheim Vetmedica; Pfizer Animal Health; Novartis Animal Health FX Supported in part by Boehringer Ingelheim Vetmedica, Pfizer Animal Health, and Novartis Animal Health. NR 26 TC 2 Z9 2 U1 1 U2 7 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 EI 1943-569X J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD JUN 15 PY 2015 VL 246 IS 12 BP 1358 EP 1362 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CP4KJ UT WOS:000359850100031 PM 26043135 ER PT J AU Siderhurst, MS Jang, EB Carvalho, LAFN Nagata, JT Derstine, NT AF Siderhurst, Matthew S. Jang, Eric B. Carvalho, Lori A. F. N. Nagata, Janice T. Derstine, Nathan T. TI Disruption of Darna pallivitta (Lepidoptera: Limacodidae) by Conventional and Mobile Pheromone Deployment SO JOURNAL OF INSECT SCIENCE LA English DT Article DE melon fly; n-butyl (E)-7,9-decadienoate; sex pheromone; invasive species; communication disruption ID MEDITERRANEAN FRUIT-FLIES; MATING DISRUPTION; ERADICATION; ORCHARDS AB Identification of the Darna pallivitta (Moore) pheromone component n-butyl (E)-7,9-decadienoate (E7,9-10: COOn-Bu) has made it possible to investigate communication disruption to control this lepidopteran pest. Conventional communication disruption trials showed marked decreases in the mean number of male moths captured in E7,9-10:COOnBu-treated fields compared with control fields. For traps baited with E7,9-10:COOnBu, percent disruptions were 94.4% and 92.1% for septa (1 g pheromone/ha, 1-wk trial duration) and spirals (6 g pheromone/ha, 8-wk trial duration) respectively. For traps baited with virgin female moths, percent disruption was 73.3% using septa disruptors (1 g pheromone/ha, 1-wk trial duration). Mobile communication disruption using Bactrocera cucurbitae (Coquillett) as carriers for E7,9-10:COOn-Bu was evaluated in the following three areas: fly survivorship, attraction of male moths to treated flies, and moth disruption in a small-scale field trial. Topical application of E7,9-10: COOnBu showed no significant decrease in survivorship at 50 and 80 mu g/fly. However, decreased survivorship was observed at 100 mu g/fly and linear regression showed E7,9-10:COOnBu dose was significantly correlated with B. cucurbitae survivorship. Traps containing honey-pheromone-fed flies attracted and caught D. pallivitta over a 1-wk period, demonstrating the attractiveness of the carrier. Releasing E7,9-10:COOnBu-fed B. cucurbitae (similar to 2 g pheromone/ha, 1-wk trial duration) resulted in significantly reduced trap catches in treatment fields compared with control fields on the first 2 d of the field trial. Percent disruptions were 84.7% (day 1) and 56.0% (day 2). These results suggest that both conventional communication disruption and mobile communication disruption have potential to control D. pallivitta. C1 [Siderhurst, Matthew S.; Derstine, Nathan T.] Eastern Mennonite Univ, Dept Chem, Harrisonburg, VA 22802 USA. [Jang, Eric B.; Carvalho, Lori A. F. N.; Nagata, Janice T.] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Siderhurst, MS (reprint author), Eastern Mennonite Univ, Dept Chem, 1200 Pk Rd, Harrisonburg, VA 22802 USA. EM matthew.siderhurst@emu.edu FU Hawaii Invasive Species Council FX We would thank Esther Schneider for her assistance in conducting field experiments and moth rearing. This project was supported in part by a grant from the Hawaii Invasive Species Council. NR 17 TC 0 Z9 0 U1 1 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUN 15 PY 2015 VL 15 AR 67 DI 10.1093/jisesa/iev052 PG 8 WC Entomology SC Entomology GA CK6ZZ UT WOS:000356380100001 ER PT J AU Bannantine, JP Stabel, JR Laws, E Cardieri, MCD Souza, CD AF Bannantine, John P. Stabel, Judith R. Laws, Elizabeth Cardieri, Maria Clara D. Souza, Cleverson D. TI Mycobacterium avium Subspecies paratuberculosis Recombinant Proteins Modulate Antimycobacterial Functions of Bovine Macrophages SO PLOS ONE LA English DT Article ID GAMMA-INTERFERON; NITRIC-OXIDE; DAIRY-COWS; TUBERCULOSIS; INTERLEUKIN-10; EXPRESSION; MATURATION; INFECTION; CATTLE; LIPOPROTEINS AB It has been shown that Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) activates the Mitogen Activated Protein Kinase (MAPK) p38 pathway, yet it is unclear which components of M. paratuberculosis are involved in the process. Therefore, a set of 42 M. paratuberculosis recombinant proteins expressed from coding sequences annotated as lipoproteins were screened for their ability to induce IL-10 expression, an indicator of MAPKp38 activation, in bovine monocyte-derived macrophages. A recombinant lipoprotein, designated as MAP3837c, was among a group of 6 proteins that strongly induced IL-10 gene transcription in bovine macrophages, averaging a 3.1-fold increase compared to non-stimulated macrophages. However, a parallel increase in expression of IL-12 and TNF-alpha was only observed in macrophages exposed to a subset of these 6 proteins. Selected recombinant proteins were further analyzed for their ability to enhance survival of M. avium within bovine macrophages as measured by recovered viable bacteria and nitrite production. All 6 IL-10 inducing MAP recombinant proteins along with M. paratuberculosis cells significantly enhanced phosphorylation of MAPK-p38 in bovine macrophages. Although these proteins are likely not post translationally lipidated in E. coli and thus is a limitation in this study, these results form the foundation of how the protein component of the lipoprotein interacts with the immune system. Collectively, these data reveal M. paratuberculosis proteins that might play a role in MAPK-p38 pathway activation and hence in survival of this organism within bovine macrophages. C1 [Bannantine, John P.; Stabel, Judith R.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Laws, Elizabeth; Cardieri, Maria Clara D.; Souza, Cleverson D.] Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA. RP Souza, CD (reprint author), Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA. EM souza@vetmed.wsu.edu OI Bannantine, John/0000-0002-5692-7898 NR 35 TC 1 Z9 1 U1 1 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 15 PY 2015 VL 10 IS 6 AR e0128966 DI 10.1371/journal.pone.0128966 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6HU UT WOS:000356329900040 PM 26076028 ER PT J AU Kertho, A Mamidi, S Bonman, JM McClean, PE Acevedo, M AF Kertho, Albert Mamidi, Sujan Bonman, J. Michael McClean, Phillip E. Acevedo, Maricelis TI Genome-Wide Association Mapping for Resistance to Leaf and Stripe Rust in Winter-Habit Hexaploid Wheat Landraces SO PLOS ONE LA English DT Article ID F-SP TRITICI; TURGIDUM SSP DICOCCOIDES; PUCCINIA-STRIIFORMIS; DISEASE-RESISTANCE; CHROMOSOMAL LOCATION; HIGH-TEMPERATURE; UNITED-STATES; LINKAGE DISEQUILIBRIUM; AESTIVUM L.; MODEL AB Leaf rust, caused by Puccinia triticina (Pt), and stripe rust, caused by P. striiformis f. sp. tritici (Pst), are destructive foliar diseases of wheat worldwide. Breeding for disease resistance is the preferred strategy of managing both diseases. The continued emergence of new races of Pt and Pst requires a constant search for new sources of resistance. Here we report a genome-wide association analysis of 567 winter wheat (Triticum aestivum) land-race accessions using the Infinium iSelect 9K wheat SNP array to identify loci associated with seedling resistance to five races of Pt (MDCL, MFPS, THBL, TDBG, and TBDJ) and one race of Pst (PSTv-37) frequently found in the Northern Great Plains of the United States. Mixed linear models identified 65 and eight significant markers associated with leaf rust and stripe rust, respectively. Further, we identified 31 and three QTL associated with resistance to Pt and Pst, respectively. Eleven QTL, identified on chromosomes 3A, 4A, 5A, and 6D, are previously unknown for leaf rust resistance in T. aestivum. C1 [Kertho, Albert; Acevedo, Maricelis] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. [Mamidi, Sujan; McClean, Phillip E.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Bonman, J. Michael] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID USA. RP Acevedo, M (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. EM maricelis.acevedo@ndsu.edu OI mamidi, sujan/0000-0002-3837-6121 FU Ducks Unlimited, Inc.; Triticeae CAP FX This work was supported by Ducks Unlimited, Inc. (http://www.ducks.org/); and Triticeae CAP (http://www.triticeaecap.org/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 11 Z9 11 U1 1 U2 14 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 15 PY 2015 VL 10 IS 6 AR e0129580 DI 10.1371/journal.pone.0129580 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6HU UT WOS:000356329900085 PM 26076040 ER PT J AU Sachdeva, S Iliev, R Ekhtiari, H Dehghani, M AF Sachdeva, Sonya Iliev, Rumen Ekhtiari, Hamed Dehghani, Morteza TI The Role of Self-Sacrifice in Moral Dilemmas SO PLOS ONE LA English DT Article ID TROLLEY PROBLEM; INTUITIONS; JUDGMENT; PSYCHOLOGY; PERCEPTION; ASYMMETRY; VALUES AB Centuries' worth of cultural stories suggest that self-sacrifice may be a cornerstone of our moral concepts, yet this notion is largely absent from recent theories in moral psychology. For instance, in the footbridge version of the well-known trolley car problem the only way to save five people from a runaway trolley is to push a single man on the tracks. It is explicitly specified that the bystander cannot sacrifice himself because his weight is insufficient to stop the trolley. But imagine if this were not the case. Would people rather sacrifice themselves than push another? In Study 1, we find that people approve of self-sacrifice more than directly harming another person to achieve the same outcome. In Studies 2 and 3, we demonstrate that the effect is not broadly about sensitivity to self-cost, instead there is something unique about sacrificing the self. Important theoretical implications about agent-relativity and the role of causality in moral judgments are discussed. C1 [Sachdeva, Sonya] US Forest Serv, Social Sci Res Unit, Evanston, IL USA. [Iliev, Rumen] Univ Michigan, Ford Sch Publ Policy, Ann Arbor, MI 48109 USA. [Ekhtiari, Hamed] Univ Tehran Med Sci, Iranian Natl Ctr Addict Studies, Res Ctr Mol & Cellular Imaging, Tehran, Iran. [Ekhtiari, Hamed] Inst Cognit Sci Studies, Translat Neurosci Program, Tehran, Iran. [Dehghani, Morteza] Univ So Calif, Dept Psychol, Los Angeles, CA 90089 USA. [Dehghani, Morteza] Univ So Calif, Brain & Creat Inst, Los Angeles, CA 90089 USA. RP Dehghani, M (reprint author), Univ So Calif, Dept Psychol, Los Angeles, CA 90089 USA. EM mdehghan@usc.edu OI Dehghani, Morteza/0000-0002-9478-4365 FU National Science Foundation [SES 0962185] FX This work was supported by National Science Foundation grant SES 0962185. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 0 Z9 0 U1 5 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 15 PY 2015 VL 10 IS 6 AR e0127409 DI 10.1371/journal.pone.0127409 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6HU UT WOS:000356329900012 PM 26075881 ER PT J AU Wang, QX Yan, DD Liu, PF Mao, LG Wang, D Fang, WS Li, Y Ouyang, CB Guo, MX Cao, AC AF Wang, Qiuxia Yan, Dongdong Liu, Pengfei Mao, Liangang Wang, Dong Fang, Wensheng Li, Yuan Ouyang, Canbin Guo, Meixia Cao, Aocheng TI Chloropicrin Emission Reduction by Soil Amendment with Biochar SO PLOS ONE LA English DT Article ID ROOT-KNOT NEMATODES; AGRICULTURAL FUMIGANTS; GREENHOUSE TOMATOES; 1,3-DICHLOROPROPENE; EFFICACY; FUMIGATION; STRAWBERRY; SORPTION; MANURE; BLACK AB Biochar has sorption capacity, and can be used to enhance the sequestration of volatile organic contaminants such as pesticides in soil. Chloropicrin (CP) is an important soil fumigant for the production of many fruit and vegetable crops, but its emissions must be minimized to reduce exposure risks and air pollution. The objective of this study was to determine the capacity of biochar to adsorb CP and the effect of biochar amendments to soil on CP emission, concentration in the soil gas phase, degradation in soil and CP bioactivity for controlling soil borne pests. CP emission and concentration in the soil air phase were measured from packed soil columns after fumigant injection at 20-cm depth and application of selected doses of biocharto the surface 5 cm soil. Laboratory incubation and fumigation experiments were conducted to determine the capacity of biochar to adsorb CP, the effects on CP degradation and, separately, CP's bioactivity on soil borne pests in soil amended with biochar. Biochar amendment at 2% to 5% (w/w) greatly reduced total CP emission losses by 85.7% - 97.7% compared to fumigation without biochar. CP concentrations in the soil gas-phase, especially in the top 5 cm of soil, were reduced within 48 h following application. The half-life of CP decreased from 13.6 h to 6.4 h as the biochar rate increased from 0% to 5%. CP and its metabolite (dichloronitromethane) both degraded more rapidly in pure biochar than in soil. The biochar used in the present study had a maximum adsorption capacity for CP of less than 5 mg g(-1). There were no negative effects on pathogen and nematode control when the biochar used in this study was less than 1% (on a weight basis) in soil. Biochar amendment to soil reduced the emissions of CP. CP concentrations in the top 5 cm of soil gas-phase were reduced. CP degradation was accelerated with the addition of biochar. The biochar used in the present study had a low adsorption capacity for CP. There were no negative effects on pathogen and nematode control when the biochar amendment rate was less than 1% (by weight). The findings would be useful for establishing guidelines for biochar use in soil fumigation. C1 [Wang, Qiuxia; Yan, Dongdong; Liu, Pengfei; Mao, Liangang; Fang, Wensheng; Li, Yuan; Ouyang, Canbin; Guo, Meixia; Cao, Aocheng] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Wang, Dong] USDA ARS, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, Parlier, DC USA. RP Cao, AC (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM caoac@vip.sina.com FU National Natural Science Foundation project of China [30900958]; Beijing Nova program [2010B064] FX This research was financially supported by the National Natural Science Foundation project of China (30900958) and Beijing Nova program (No. 2010B064). NR 34 TC 2 Z9 2 U1 5 U2 42 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 15 PY 2015 VL 10 IS 6 AR e0129448 DI 10.1371/journal.pone.0129448 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6HU UT WOS:000356329900075 PM 26075904 ER PT J AU Martin, MK Helm, J Patyk, KA AF Martin, Michael K. Helm, Julie Patyk, Kelly A. TI An approach for de-identification of point locations of livestock premises for further use in disease spread modeling SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Modeling; Simulation; K-anonymity; Privacy; Confidentiality; Random permutation ID MOUTH-DISEASE; PRIVACY PROTECTION; SIMULATION-MODEL; K-ANONYMITY; HEALTH DATA; OUTBREAK; IMPACT; AUSTRALIA AB We describe a method for de-identifying point location data used for disease spread modeling to allow data custodians to share data with modeling experts without disclosing individual farm identities. The approach is implemented in an open-source software program that is described and evaluated here. The program allows a data custodian to select a level of de-identification based on the K-anonymity statistic. The program converts a file of true farm locations and attributes into a file appropriate for use in disease spread modeling with the locations randomly modified to prevent re-identification based on location. Important epidemiological relationships such as clustering are preserved to as much as possible to allow modeling similar to those using true identifiable data. The software implementation was verified by visual inspection and basic descriptive spatial analysis of the output. Performance is sufficient to allow de-identification of even large data sets on desktop computers available to any data custodian. (C) 2015 Elsevier B.V. All rights reserved. C1 [Martin, Michael K.; Helm, Julie] Clemson Univ, Livestock Poultry Hlth Div, Columbia, SC 29224 USA. [Patyk, Kelly A.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Sci Technol & Anal Serv,Ctr Epidemiol & Anim Hlth, Ft Collins, CO 80526 USA. RP Martin, MK (reprint author), Clemson Univ, Livestock Poultry Hlth Div, POB 102406, Columbia, SC 29224 USA. EM mmarti5@clemson.edu OI Martin, Michael/0000-0002-8764-8525 FU USDA [13-9208-0328-CA] FX The authors thank the South Carolina Animal Disease Traceability Steering Committee and the South Carolina Poultry Health Advisory Committee for their thoughtful consideration and approval of the techniques described here. We are also grateful to Dr. Tim C. Boyer, Dr. Kimberly N. Forde-Folle and Dr. Bruce Wagner for their helpful comments and suggestions, and to Anne Berry for assistance with the manuscript. The authors would also like to acknowledge the support for this work under USDA Cooperative Agreement #13-9208-0328-CA. NR 45 TC 1 Z9 1 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD JUN 15 PY 2015 VL 120 IS 2 BP 131 EP 140 DI 10.1016/j.prevetmed.2015.04.010 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA CL0OU UT WOS:000356642800001 PM 25944175 ER PT J AU Mossavar-Rahmani, Y Shaw, PA Wong, WW Sotres-Alvarez, D Gellman, MD Van Horn, L Stoutenberg, M Daviglus, ML Wylie-Rosett, J Siega-Riz, AM Ou, FS Prentice, RL AF Mossavar-Rahmani, Yasmin Shaw, Pamela A. Wong, William W. Sotres-Alvarez, Daniela Gellman, Marc D. Van Horn, Linda Stoutenberg, Mark Daviglus, Martha L. Wylie-Rosett, Judith Siega-Riz, Anna Maria Ou, Fang-Shu Prentice, Ross L. TI Applying Recovery Biomarkers to Calibrate Self-Report Measures of Energy and Protein in the Hispanic Community Health Study/Study of Latinos SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE biological markers; calibration equations; dietary measurement error; Hispanics/Latinos; 24-hour dietary recall; nutrition assessment ID DOUBLY-LABELED WATER; POSTMENOPAUSAL WOMEN; URINE; EXPENDITURE; RISK; ASSOCIATIONS; BACKGROUNDS; CONSUMPTION; SAMPLES; DESIGN AB We investigated measurement error in the self-reported diets of US Hispanics/Latinos, who are prone to obesity and related comorbidities, by background (Central American, Cuban, Dominican, Mexican, Puerto Rican, and South American) in 2010-2012. In 477 participants aged 18-74 years, doubly labeled water and urinary nitrogen were used as objective recovery biomarkers of energy and protein intakes. Self-report was captured from two 24-hour dietary recalls. All measures were repeated in a subsample of 98 individuals. We examined the bias of dietary recalls and their associations with participant characteristics using generalized estimating equations. Energy intake was underestimated by 25.3% (men, 21.8%; women, 27.3%), and protein intake was underestimated by 18.5% (men, 14.7%; women, 20.7%). Protein density was overestimated by 10.7% (men, 11.3%; women, 10.1%). Higher body mass index and Hispanic/Latino background were associated with underestimation of energy (P < 0.05). For protein intake, higher body mass index, older age, nonsmoking, Spanish speaking, and Hispanic/Latino back-ground were associated with underestimation (P < 0.05). Systematic underreporting of energy and protein intakes and overreporting of protein density were found to vary significantly by Hispanic/Latino background. We developed calibration equations that correct for subject-specific error in reporting that can be used to reduce bias in diet-disease association studies. C1 [Mossavar-Rahmani, Yasmin; Wylie-Rosett, Judith] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10461 USA. [Shaw, Pamela A.] Univ Penn, Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [Wong, William W.] ARS, USDA, Houston, TX USA. [Wong, William W.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Sotres-Alvarez, Daniela; Ou, Fang-Shu] Univ N Carolina, Dept Biostat, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Gellman, Marc D.] Univ Miami, Dept Psychol, Coral Gables, FL 33124 USA. [Van Horn, Linda] Northwestern Univ, Dept Prevent Med, Feinberg Sch Med, Chicago, IL 60611 USA. [Stoutenberg, Mark] Univ Miami, Miller Sch Med, Dept Publ Hlth Sci, Miami, FL 33136 USA. [Daviglus, Martha L.] Univ Illinois, Inst Minor Hlth Res, Chicago, IL USA. [Siega-Riz, Anna Maria] Univ N Carolina, Dept Nutr, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Siega-Riz, Anna Maria] Univ N Carolina, Dept Epidemiol, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Prentice, Ross L.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. RP Mossavar-Rahmani, Y (reprint author), Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Div Hlth Promot & Nutr Res, 1300 Morris Pk Ave,Block Bldg,Room 339, Bronx, NY 10461 USA. EM yasmin.mossavar-rahmani@einstein.yu.edu RI DeMarcus, Forrest/O-9190-2016; Sotres-Alvarez, Daniela/O-9085-2016 OI Sotres-Alvarez, Daniela/0000-0002-3226-6140 FU National Heart, Lung, and Blood Institute [11414319-R01HL095856, NO1-HC65233, N01-HC65234, N01-HC65235, N01-HC65236, N01-HC65237]; National Center for Advancing Translational Sciences at the National Institutes of Health [UL1 TR001073]; National Cancer Institute [CA53996]; National Heart, Lung, and Blood Institute: the National Center on Minority Health and Health Disparities; National Heart, Lung, and Blood Institute: National Institute of Deafness and Other Communications Disorders; National Heart, Lung, and Blood Institute: National Institute of Dental and Craniofacial Research; National Heart, Lung, and Blood Institute: National Institute of Diabetes and Digestive and Kidney Diseases; National Heart, Lung, and Blood Institute: National Institute of Neurological Disorders and Stroke; National Heart, Lung, and Blood Institute: Office of Dietary Supplements FX This work was supported by grant 11414319-R01HL095856 from the National Heart, Lung, and Blood Institute. The Hispanic Community Health Study/Study of Latinos was carried out as a collaborative study supported by contracts from the National Heart, Lung, and Blood Institute to the University of North Carolina (NO1-HC65233), University of Miami (N01-HC65234), Albert Einstein College of Medicine (N01-HC65235), Northwestern University (N01-HC65236), and San Diego State University (N01-HC65237). The following contribute to the Hispanic Community Health Study/Study of Latinos through a transfer of funds to the National Heart, Lung, and Blood Institute: the National Center on Minority Health and Health Disparities, the National Institute of Deafness and Other Communications Disorders, the National Institute of Dental and Craniofacial Research, the National Institute of Diabetes and Digestive and Kidney Diseases, the National Institute of Neurological Disorders and Stroke, and the Office of Dietary Supplements. Additional support at the Albert Einstein College of Medicine was provided from the Clinical and Translational Science Award (UL1 TR001073) from the National Center for Advancing Translational Sciences at the National Institutes of Health, and support at the Fred Hutchinson Cancer Research Center was provided from National Cancer Institute grant CA53996. NR 26 TC 8 Z9 8 U1 1 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 EI 1476-6256 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JUN 15 PY 2015 VL 181 IS 12 BP 996 EP 1007 DI 10.1093/aje/kwu468 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CK4GE UT WOS:000356180600009 PM 25995289 ER PT J AU McRoberts, RE Naesset, E Gobakken, T AF McRoberts, Ronald E. Naesset, Erik Gobakken, Terje TI Optimizing the k-Nearest Neighbors technique for estimating forest aboveground biomass using airborne laser scanning data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Distance metric; Precision ID REMOTELY-SENSED DATA; SATELLITE IMAGERY; VARIABLES; AREA; INVENTORIES; PREDICTIONS; INFERENCE; VOLUME; DENSITY; FIELD AB Nearest neighbors techniques calculate predictions as linear combinations of observations for a selected number of population units in a sample that are most similar, or nearest, in a space of auxiliary variables to the population unit requiring the prediction. Nearest neighbors techniques have been shown to be particularly effective when used with forest inventory and remotely sensed data. Recent attention has focused on developing an underlying foundation consisting of diagnostic tools, inferential extensions, and techniques for optimization. For a study area in Norway, forest inventory and airborne laser scanning data were used with the k-Nearest Neighbors technique to estimate mean aboveground biomass per unit area. Optimization entailed reduction of the dimension of feature space, deletion of influential outliers, and selection of optimal weights for the weighted Euclidean distance metric. These optimization steps increased the proportion of variability explained in the reference set by as much as 20%, reduced confidence interval widths by as much as 35%, and produced standard errors that were as small as 3% of the estimate of the mean. Published by Elsevier Inc. C1 [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Naesset, Erik; Gobakken, Terje] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, As, Norway. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us FU COST Action [FP1001] FX The authors thank the following for careful reviews and insightful comments on the paper: Professor Gherardo Chirici, University of Molise, Italy; Drs. Daniel McInerney and Frank Barrett, Irish Forest Service; Dr. Ambrose Berger, Federal Research and Training Centre for Forests, Natural Hazards and Landscape, Austria; and Professor Erkki Tomppo, Finnish Forest Research Institute. Funding for the Task Force meeting at which much of the material in this article was first presented was provided by COST Action FP1001. NR 45 TC 14 Z9 14 U1 1 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN 15 PY 2015 VL 163 BP 13 EP 22 DI 10.1016/j.rse.2015.02.026 PG 10 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CJ8RN UT WOS:000355771300002 ER PT J AU Wing, BM Ritchie, MW Boston, K Cohen, WB Olsen, MJ AF Wing, Brian M. Ritchie, Martin W. Boston, Kevin Cohen, Warren B. Olsen, Michael J. TI Individual snag detection using neighborhood attribute filtered airborne lidar data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Snags; Snag detection; Snag density; Airborne lidar; Neighborhood attribute lidar filtering; Lidar filtering; Forestry ID PINE FOREST; INVENTORY; TREES; DEAD; PREDICTION; MORTALITY; ACCURACY; DENSITY; IMAGERY; HEIGHT AB The ability to estimate and monitor standing dead trees (snags) has been difficult due to their irregular and sparse distribution, often requiring intensive sampling methods to obtain statistically significant estimates. This study presents a new method for estimating and monitoring snags using neighborhood attribute filtered airborne discrete-return lidar data. The method first develops and then applies an automated filtering algorithm that utilizes three dimensional neighborhood lidar point-based intensity and density statistics to remove lidar points associated with live trees and retain lidar points associated with snags. A traditional airborne lidar individual-tree detection procedure is then applied to the snag-filtered lidar point cloud, resulting in stem map of identified snags with height estimates. The filtering algorithm was developed using training datasets comprised of four different forest types in wide range of stand conditions, and then applied to independent data to determine successful snag detection rates. Detection rates ranged from 43 to 100%, increasing as the size of snags increased. The overall detection rate for snags with DBH >= 25 cm was 56% (+/- 2.9%) with low commission error rates. The method provides the ability to estimate snag density and stem map a large proportion of snags across the landscape. The resulting information can be used to analyze the spatial distribution of snags, provide a better understanding of wildlife snag use dynamics, assess achievement of stocking standard requirements, and bring more clarity to snag stocking standards. Published by Elsevier Inc. C1 [Wing, Brian M.; Ritchie, Martin W.] US Forest Serv, USDA, Pacific Southwest Res Stn, Redding, CA 96002 USA. [Boston, Kevin] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Cohen, Warren B.] US Forest Serv, USDA, Pacific Southwest Res Stn, Corvallis, OR 97731 USA. [Olsen, Michael J.] Oregon State Univ, Sch Civil & Construct Engn, Corvallis, OR 97331 USA. RP Wing, BM (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM brianwing@fs.fed.us; mritchie@fs.fed.us; kevin.boston@oregonstate.edu; warren.cohen@oregonstate.edu; michael.olsen@oregonstate.edu NR 64 TC 9 Z9 9 U1 2 U2 22 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN 15 PY 2015 VL 163 BP 165 EP 179 DI 10.1016/j.rse.2015.03.013 PG 15 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CJ8RN UT WOS:000355771300015 ER PT J AU Lamba, J Karthikeyan, KG Thompson, AM AF Lamba, Jasmeet Karthikeyan, K. G. Thompson, A. M. TI Using radiometric fingerprinting and phosphorus to elucidate sediment transport dynamics in an agricultural watershed SO HYDROLOGICAL PROCESSES LA English DT Article DE sediment sources; suspended sediment; sediment fingerprinting; land use; phosphorus; sediment resuspension ID FLUVIAL SUSPENDED SEDIMENT; EFFECTS ASSESSMENT PROJECT; FALLOUT RADIONUCLIDES; RIVER-BASINS; BE-7; BERYLLIUM-7; CATCHMENT; EROSION; PB-210; POWER AB The major goals of this study were to determine stream bed sediment erosion/deposition rates, sediment age, percent new' sediment, and suspended sediment origin during two storm events of contrasting magnitudes (11.9mm over 5h and 58.9mm over 39h) using fallout radionuclides (excess lead 210 - Pb-210(xs) and beryllium 7 - Be-7) and link the nature and type of sediment source contributions to potential phosphorus (P) off-site transport. The study was conducted in cropland-dominated and mixed land use subwatersheds in the non-glaciated Pleasant Valley watershed (50km(2)) in South Central Wisconsin. Fine sediment deposition and erosion rates on stream beds varied from 0.76 to 119.29mgcm(-2)day(-1) (at sites near the watershed outlet) and 1.72 to 7.72mgcm(-2)day(-1) (at sites in the headwaters), respectively, during the two storm events. The suspended sediment age ranged from 123 +/- 12 to 234 +/- 33days during the smaller storm event; however, older sediment was more prevalent (p=0.037) in the streams during the larger event with suspended sediment age ranging from 226 +/- 9 to 322 +/- 114days. During the small and large storm event, percent new sediment in suspended sediment ranged from 5.3 +/- 2.1 to 21.0 +/- 2.9% and 5.3 +/- 2.7 to 6.7 +/- 5.7%, respectively. In the cropland-dominated subwatershed, upland soils were the major source of suspended sediment, whereas in the mixed land use subwatershed, both uplands and stream banks had relatively similar contributions to suspended sediment. In-stream (suspended and bed) sediment P levels ranged from 703 +/- 193 to 963 +/- 84mgkg(-1) during the two storm events. The P concentrations in suspended and bed sediment were reflective of the dominant sediment source (upland or stream bank or mixed). Overall, sediment transport dynamics showed significant variability between subwatersheds of different land use characteristics during two contrasting storm events. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Lamba, Jasmeet; Karthikeyan, K. G.; Thompson, A. M.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. RP Lamba, J (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702 Curtin Rd, University Pk, PA 16802 USA. EM jul59@psu.edu RI LAMBA, JASMEET/B-3878-2015 FU USDA NIFA award [2009-51130-06049] FX Funding for this project was provided by USDA NIFA award #2009-51130-06049. We thank John Panuska, Zach Zopp, Harsh Singh, Laura Good, Graham Peaslee, and staff at Dane County Land Conservation Division, Dane County, Wisconsin for their assistance in this study. We also thank all the producers in this watershed for allowing soil sampling from their lands. NR 49 TC 2 Z9 2 U1 1 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD JUN 15 PY 2015 VL 29 IS 12 BP 2681 EP 2693 DI 10.1002/hyp.10396 PG 13 WC Water Resources SC Water Resources GA CJ7TD UT WOS:000355701700003 ER PT J AU Xiong, LH Chen, K Ke, FS Lou, HB Yue, GQ Shen, B Dong, F Wang, SY Chen, LY Wang, CZ Ho, KM Wang, XD Lai, LH Xie, HL Xiao, TQ Jiang, JZ AF Xiong, L. H. Chen, K. Ke, F. S. Lou, H. B. Yue, G. Q. Shen, B. Dong, F. Wang, S. Y. Chen, L. Y. Wang, C. Z. Ho, K. M. Wang, X. D. Lai, L. H. Xie, H. L. Xiao, T. Q. Jiang, J. Z. TI Structural and dynamical properties of liquid Ag74Ge26 alloy studied by experiments and ab initio molecular dynamics simulation SO ACTA MATERIALIA LA English DT Article DE Ag74Ge26 alloy; Liquid structure; Ab initio molecular dynamics; X-ray diffraction ID METALLIC GLASSES; ORDER; TRANSITION; WATER; DENSITY; SILVER AB The structures and dynamics of liquid Ag74Ge26 alloy at different temperatures were investigated by high-energy X-ray diffraction and ab initio molecular dynamics simulation. The calculated structure factors agree well with the experimental data. Local atomic structure orders have been characterized by angular distribution functions, Honeycutt-Andersen index, Voronoi tessellation and the atomic cluster alignment. We reveal that (i) < 0,3,6,4,0 >, < 0,2,8,4,0 >, < 0,3,6,5,0 >, and < 1,3,4,5,1 > polyhedral dominate in the undercooled Ag74Ge26 liquid. (ii) The icosahedral-like short-range order is enhanced as the temperature decreases while < 0,3,6,4,0 >, < 0,2,8,4,0 >, < 0,3,6,5,0 >, and < 0,1,10,2,0 > polyhedral become the major short-range orders at low temperature. (iii) The medium-range order which was formed for amorphous phase at ambient temperature persists in the undercooled liquid. In addition, it is found that the diffusion constant of Ag is lower than that of Ge over the studied temperature range. Our findings elucidate that highly dominated short-range order associated with nearest-neighbors shell could empower the development of medium-range order and the formation of metallic glasses. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. C1 [Xiong, L. H.; Lou, H. B.; Wang, X. D.; Jiang, J. Z.] Zhejiang Univ, State Key Lab Silicon Mat, Lab New Struct Mat, Int Ctr New Struct Mat, Hangzhou 310027, Zhejiang, Peoples R China. [Xiong, L. H.; Lou, H. B.; Wang, X. D.; Jiang, J. Z.] Zhejiang Univ, Sch Mat Sci & Engn, Hangzhou 310027, Zhejiang, Peoples R China. [Chen, K.; Ke, F. S.; Yue, G. Q.; Shen, B.; Dong, F.; Wang, S. Y.; Chen, L. Y.] Fudan Univ, Shanghai Ultra Precis Opt Mfg Engn Ctr, Shanghai 200433, Peoples R China. [Chen, K.; Ke, F. S.; Yue, G. Q.; Shen, B.; Dong, F.; Wang, S. Y.; Chen, L. Y.] Fudan Univ, Dept Opt Sci & Engn, Shanghai 200433, Peoples R China. [Wang, S. Y.; Wang, C. Z.; Ho, K. M.] Iowa State Univ, USDA, Ames Lab, Ames, IA 50011 USA. [Wang, S. Y.; Wang, C. Z.; Ho, K. M.] Iowa State Univ, Dept Phys & Astron, Ames, IA 50011 USA. [Lai, L. H.; Xie, H. L.; Xiao, T. Q.] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201203, Peoples R China. RP Wang, SY (reprint author), Fudan Univ, Shanghai Ultra Precis Opt Mfg Engn Ctr, Shanghai 200433, Peoples R China. EM songyouwang@fudan.edu.cn; jiangjz@zju.edu.cn RI Wang, Songyou/H-4529-2011; Xiong, Lianghua/S-7960-2016 OI Wang, Songyou/0000-0002-4249-3427; Xiong, Lianghua/0000-0001-5323-6525 FU National Basic Research Program of China [2012CB825700, 2010CB933703, 2012CB934303]; National Natural Science Foundation of China [51371157, U1432105, 11374055, 10974029]; Fudan High-end Computing Center; Fundamental Research Funds for the Central Universities; US Department of Energy, Basic Energy Sciences, and Division of Materials Science and Engineering [DE-AC02-07CH11358] FX Financial supports from the National Basic Research Program of China (2012CB825700, 2010CB933703, and 2012CB934303), National Natural Science Foundation of China (51371157, U1432105, 11374055 and 10974029), the Fudan High-end Computing Center, and the Fundamental Research Funds for the Central Universities are gratefully acknowledged. Work at Ames Laboratory was supported by the US Department of Energy, Basic Energy Sciences, and Division of Materials Science and Engineering, including a grant of computer time at the National Energy Research Scientific Computing Centre (NERS) in Berkeley, CA under Contract No. DE-AC02-07CH11358. NR 32 TC 1 Z9 1 U1 6 U2 61 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1359-6454 EI 1873-2453 J9 ACTA MATER JI Acta Mater. PD JUN 15 PY 2015 VL 92 BP 109 EP 116 DI 10.1016/j.actamat.2015.03.047 PG 8 WC Materials Science, Multidisciplinary; Metallurgy & Metallurgical Engineering SC Materials Science; Metallurgy & Metallurgical Engineering GA CI8XI UT WOS:000355054600012 ER PT J AU Avina-Padilla, K de la Vega, OM Rivera-Bustamante, R Martinez-Soriano, JP Owens, RA Hammond, RW Vielle-Calzada, JP AF Avina-Padilla, Katia Martinez de la Vega, Octavio Rivera-Bustamante, Rafael Pablo Martinez-Soriano, Juan Owens, Robert A. Hammond, Rosemarie W. Vielle-Calzada, Jean-Philippe TI In silico prediction and validation of potential gene targets for pospiviroid-derived small RNAs during tomato infection SO GENE LA English DT Article DE Comparative genomics; RNA-mediated gene regulation; Viroid pathogenesis; Non-coding RNA; Tomato planta macho viroid ID TUBER VIROID RNA; HOST INTERACTIONS; MESSENGER-RNA; PROTEIN VIRP1; ACCUMULATION; CLEAVAGE; PLANTS; PATHOGENICITY; INTERFERENCE; PATHOGENESIS AB Viroids are small, covalently closed, circular non-coding RNA pathogens of flowering plants. It is proposed that the symptoms of viroid pathogenesis result from a direct interaction between the viroid genomic RNA and unknown host plant factors. Using a comparative genomic approach we took advantage of the detailed annotation of the Arabidopsis thaliana (Arabidopsis) genome to identify sequence homologies between putative viroid-derived small RNAs (vd-sRNAs) and coding regions in the plant genome. A pool of sequence homologies among 29 species of the Pospiviroidae family and the Arabidopsis genome was analyzed. Using this strategy we identified putative host gene targets that may be involved in symptom expression in viroid-infected plants. In this communication, we report the in silica prediction and the experimental validation of pospiviroid-derived sRNAs conserved in the lower strand of the pathogenicity domain of seven viroid species infecting tomato; those vd-sRNAs targeted for cleavage the host mRNA encoding a conserved tomato WD40-repeat protein (SolWD40-repeat; SGN_U563134). Analysis of SolWD40-repeat expression indicated that this gene is down-regulated in tomato plants infected with tomato planta macho viroid (TPMVd). Furthermore, 5' RLM-RACE revealed that the SolWD40-repeat mRNA is cleaved at the predicted target site showing complementarity to a corresponding TPMVd-sRNA identified in silica. Our approach proved to be useful for the identification of natural host genes containing sequence homologies with segments of the Pospiviroidae genome. Using this strategy we identified a functionally conserved gene in Arabidopsis and tomato, whose expression was modified during viroid infection in the host genome; regulation of this gene expression could be guided by vd-sRNA:mRNA complementarity, suggesting that the comparison of the Arabidopsis genome to viroid sequences could lead to the identification of unexpected interactions between viroid RNAs and their host. Published by Elsevier B.V. C1 [Avina-Padilla, Katia; Martinez de la Vega, Octavio; Rivera-Bustamante, Rafael; Pablo Martinez-Soriano, Juan; Vielle-Calzada, Jean-Philippe] IPN, Ctr Invest & Estudios Avanzados, Unidad Irapuato, Guanajuato 36821, Mexico. [Avina-Padilla, Katia; Owens, Robert A.; Hammond, Rosemarie W.] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Hammond, RW (reprint author), ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM kap@ira.cinvestav.mx; omartine@ira.cinvestav.mx; rrivera@ira.cinvestav.mx; owensj301@hotmail.com; rose.hammond@ars.usda.gov; vielle@ira.cinvestav.mx RI Rivera-Bustamante, Rafael/B-4688-2009; Martinez, Octavio/F-3951-2014 OI Rivera-Bustamante, Rafael/0000-0002-5114-2370; Martinez, Octavio/0000-0002-7590-0041 FU CONACyT [210296] FX We thank Juan Carlos Ochoa Sanchez, Dr. Wei Wei, Jonathan Shao, and John C. Hammond for their assistance with figures and data processing. We thank Dr. John Hammond (USDA ARS) for his critical reading of the manuscript. KAP was funded by grant No. 210296 from CONACyT for graduate program studies. NR 74 TC 9 Z9 9 U1 0 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD JUN 15 PY 2015 VL 564 IS 2 BP 197 EP 205 DI 10.1016/j.gene.2015.03.076 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA CI8SX UT WOS:000355043100012 PM 25862922 ER PT J AU Spear, A Faaberg, KS AF Spear, Allyn Faaberg, Kay S. TI Development of a genome copy specific RT-qPCR assay for divergent strains of type 2 porcine reproductive and respiratory syndrome virus SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE PRRSV; RT-qPCR; Genome copy; nsp9 ID HETEROCLITE SUBGENOMIC RNAS; PCR ASSAY; INFECTION; PRRSV; CHINESE; SWINE AB Porcine reproductive and respiratory syndrome virus (PRRSV) became a significant pathogen of swine upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, constantly re-emerging pathogen. In addition to the challenges faced in development of vaccines and diagnostics, research on the basic molecular pathogenesis of PRRSV is also restrained by the ability to accurately and comparatively quantitate levels of replication in different tissues and between strains. This is further complicated by the presence of non-genomic RNA within infected tissues which are generally detected with equivalent efficiency by RT-qPCR based techniques, thereby introducing inherent error in these measurements that may differ significantly by tissue and strain. To address this, an RT-qPCR based technique was developed which targets the viral RNA-dependent RNA polymerase gene (nsp9) which is unique to genomic RNA, being absent from all subgenomic and heteroclite RNAs. This assay targets a region of considerable sequence conservation, and based on sequence only, should be quantitative for approximately 40% of all Type 2 PRRSV strains in GenBank for which nsp9 sequence is available. The assay was demonstrated to be linear over nine orders of magnitude (10(10)-10(2) copies) and can be readily adapted for multiplex detection of additional divergent PRRSV strains. This assay will add significantly to the ability to assess and compare PRRSV replication in a variety of tissues and between divergent strains, including highly pathogenic strains of considerable concern to the global pork industry. Published by Elsevier B.V. C1 [Faaberg, Kay S.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Spear, Allyn] USDA ARS, Sch Freshwater Sci, Milwaukee, WI 53204 USA. RP Spear, A (reprint author), USDA ARS, Sch Freshwater Sci, 600 E Greenfield Ave, Milwaukee, WI 53204 USA. EM allyn.spear@ars.usda.gov NR 16 TC 0 Z9 1 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD JUN 15 PY 2015 VL 218 BP 1 EP 6 DI 10.1016/j.jviromet.2015.02.007 PG 6 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA CI1KG UT WOS:000354502500001 PM 25766790 ER PT J AU Telander, AC Slesak, RA D'Amato, AW Palik, BJ Brooks, KN Lenhart, CF AF Telander, Andrew C. Slesak, Robert A. D'Amato, Anthony W. Palik, Brian J. Brooks, Kenneth N. Lenhart, Christian F. TI Sap flow of black ash in wetland forests of northern Minnesota, USA: Hydrologic implications of tree mortality due to emerald ash borer SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Agrilus planipennis; Soil moisture; Sapwood depth; Forest management ID DIFFUSE-POROUS TREES; PLANT WATER-USE; FLUX-DENSITY; CANOPY CONDUCTANCE; LEAF-AREA; DISSIPATION PROBES; SAPWOOD AREA; PINE FOREST; TRANSPIRATION; MODEL AB Black ash (Fraxinus nigra) mortality caused by the invasive emerald ash borer (EAB) is of concern to land managers in the upper Great Lakes region, given the large areas of ash-dominated forest and potential alteration of wetland hydrology following loss of this foundation tree species. The importance of changes in evapotranspiration (ET) following black ash mortality is currently unknown and is the focus of this study. Sap flux density rates were evaluated at three black ash stands with differing moisture regimes within the Chippewa National Forest, Minnesota, USA using the Granier thermal dissipation method. Sapwood area and sap flux density were combined to determine sap flow. Tree level sap flux density estimates were comparable to other reported values and averaged 4.59, 2.31, and 1.62 m(3) m(-2) day(-1), respectively, for the very wet, wet, and moderately wet field sites. However, black ash exhibited small sapwood area in general, resulting in lower overall sap flow values. Scaled stand-level transpiration followed a similar trend as the tree-level estimates; mean daily transpiration over 10 weeks was 1.62 (80% of PET), 1.15 (53% of PET), and 0.90 (42% of PET) mm for the very wet, wet, and moderately wet site, respectively. Sap flux density was positively related to vapor pressure deficit when soil moisture was at or near saturation and negatively related when soil moisture content was lower. There was also a significant positive relationship between sap flux density and relative soil moisture saturation at the stand scale. Our results indicate that hydrologic regime has substantial influence on sap flow with highest transpiration when soil moisture is at saturation, underscoring the unique ecological role that black ash plays in these wetland forest types. The effects of EAB-induced black ash mortality on overall ET and related hydrologic processes will likely be greatest in the wettest hydrologic regimes. (C) 2015 Elsevier B.V. All rights reserved. C1 [Telander, Andrew C.; Slesak, Robert A.; D'Amato, Anthony W.; Brooks, Kenneth N.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Slesak, Robert A.] Minnesota Forest Resources Council, St Paul, MN 55108 USA. [Palik, Brian J.] US Forest Serv, USDA, Northern Res Stn, Grand Rapids, MN 55744 USA. [Lenhart, Christian F.] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA. RP Slesak, RA (reprint author), 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. EM raslesak@umn.edu FU Minnesota Environment and Natural Resources Trust Fund; Minnesota Forest Resources Council; USDA Forest Service Northern Research Station FX This project was funded by the Minnesota Environment and Natural Resources Trust Fund, the Minnesota Forest Resources Council, and the USDA Forest Service Northern Research Station. We would like to thank Steve Sebestyen and Donna Olson for providing data used in the PET calculations. We would also like to thank Joe Shannon for his assistance with project development and data interpretation, and Christopher Looney for providing the LAI estimates reported in Table 1. Assistance with field work and data collection was provided by David Rudolph. NR 53 TC 1 Z9 1 U1 9 U2 65 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 15 PY 2015 VL 206 BP 4 EP 11 DI 10.1016/j.agrformet.2015.02.019 PG 8 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA CH6IL UT WOS:000354140200002 ER PT J AU Wood, JD Griffis, TJ Baker, JM AF Wood, J. D. Griffis, T. J. Baker, J. M. TI Detecting drift bias and exposure errors in solar and photosynthetically active radiation data SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Radiation exposure; Sensor fading; Drift detection; Change point ID THERMAL OFFSET; CARBON-DIOXIDE; EARTHS SURFACE; UNITED-STATES; ENERGY; PYRANOMETERS; VARIABILITY; ABSORPTION; NETWORK; BALANCE AB All-black thermopile pyranometers are commonly used to measure solar radiation. Ensuring that the sensors are stable and free of drift is critical to accurately measure small variations in global solar irradiance at the Earth's surface (K down arrow), which is a potential driver of changes in surface temperature. We demonstrate that the decreased responsivities of Eppley PSP pyranometers of -1.5%y(-1), or -0.38% (GJ m(-2))(-1), were accompanied by a change in its spectral response owing to a discoloration of the sensing element. These observations motivated further work to develop routines to detect probable pyranometer drift in historical time-series. The temporal trends in the following ratios were used to detect pyranometer sensor drift: photosynthetically active radiation (PAR) to K down arrow, K down arrow to K-EX (extraterrestrial radiation at the top of the atmosphere) and PAR to K-EX. Data from 8 AmeriFlux sites spanning latitudes from similar to 32 to 54 degrees N were examined in this analysis. Probable drift in either a pyranometer or PAR sensor was identified at 5 of the 8 sites. The magnitude of the drift represented changes of 0.15-0.85%y(-1), which is sufficient to obscure actual trends in K down arrow, although these should be considered conservative low end drift estimates, given that we were not making comparisons to co-located higher grade instruments. Deployment exposure errors caused by sensor shading were also discovered by comparing the daily correlations between (i) K down arrow and K-EX and (ii) PAR and K-EX. Sensors drifting at rates similar to our defective PSP over a 5 year period would contribute to an underestimation of available energy of similar to 70 W m(-2), which is non-trivial in the context of assessing eddy covariance energy balance closure, employing Penman-Monteith or Bowen ratio methods or calculating albedo radiative forcings. Given that probable drift was identified at multiple AmeriFlux sites, we recommend enhancing network access to calibration services that are traceable to a high quality gold standard. (C) 2015 Elsevier B.V. All rights reserved. C1 [Wood, J. D.; Griffis, T. J.; Baker, J. M.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55105 USA. [Baker, J. M.] USDA ARS, Soil & Water Res Unit, St Paul, MN USA. RP Wood, JD (reprint author), Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55105 USA. EM jdwood@umn.edu RI Griffis, Timothy/A-5707-2011 FU United States Department of Agriculture [USDA-NIFA 2013-67019-21364]; Minnesota Corn Research & Promotion Council [4101-14SP]; United States Department of Energy's Office of Science FX We would like to acknowledge the technical support provided by Bill Breiter, as well as the site PI's from other AmeriFlux sites: T.A. Black, P. Blanken, T. Meyers, R. Scott and R. Staebler. Support for this research was provided by the United States Department of Agriculture, Grant number: USDA-NIFA 2013-67019-21364 and the Minnesota Corn Research & Promotion Council (4101-14SP). We would also like to acknowledge the AmeriFlux core site funding provided by the United States Department of Energy's Office of Science. NR 33 TC 0 Z9 0 U1 1 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 15 PY 2015 VL 206 BP 33 EP 44 DI 10.1016/j.agrformet.2015.02.015 PG 12 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA CH6IL UT WOS:000354140200004 ER PT J AU Yan, WJ Wang, YL Zhuang, H Zhang, JH AF Yan, Wenjing Wang, Yongli Zhuang, Hong Zhang, Jianhao TI DNA-engineered chiroplasmonic heteropyramids for ultrasensitive detection of mercury ion SO BIOSENSORS & BIOELECTRONICS LA English DT Article DE Gold nanoparticles; Heteropyramids; Circular dichroism; Mercury ion ID PLASMONIC CIRCULAR-DICHROISM; GOLD NANOPARTICLES; NANOROD ASSEMBLIES; OPTICAL-ACTIVITY; AU NANOROD; SENSOR; FLUORESCENCE; CHIRALITY AB In this study, plasmonic heteropyramids (HPs) made from two different sized gold nanoparticles (Au NPs) and five ssDNA sequences and their application for ultrasensitive detection of mercury ion (Hg2+) were demonstrated. Four ssDNA sequences were used as building blocks to form a pyramidal DNA frame, which contains a T-rich probe DNA at one vertex and three sulfhydryl groups modified with 10 nm Au NPs at the other three vertices. Another T-rich DNA sequence was modified and attached to a 25nm Au NP. In the presence of Hg2+ ions, 25 nm Au NPs hybridized with pyramidal DNA frame to build the plasmonic HPs based on T-Hg2+-T interaction, which exhibits unprecedented circular dichroism (CD) signal in the visible region. Based on this mechanism, a simple, high sensitive and selective chiroplasmonic HPs-based probe was constructed and demonstrated for Hg2+ ions detection. Under optimized conditions, Hg2+ ions could be selectively detected in a concentration range from 1 to 500 pg mL(-1) with a limit of detection of 0.2 pg mL(-1), which is much lower than the strictest Hg2+ safety requirement of 1 ng mL(-1) in water. (C) 2015 Elsevier B.V. All rights reserved. C1 [Yan, Wenjing; Wang, Yongli; Zhang, Jianhao] Nanjing Agr Univ, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. [Zhuang, Hong] ARS, Qual & Safety Assessment Res Unit, USDA, Athens, GA 30605 USA. RP Zhang, JH (reprint author), Nanjing Agr Univ, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. EM nau_zjh@njau.edu.cn FU National Technology Support Program of China [2012BAD28B01]; International Technology Cooperation Program of Jiangsu Province, China [BZ2014034] FX This study was sponsored by the National Technology Support Program of China (Grant no. 2012BAD28B01), the International Technology Cooperation Program of Jiangsu Province, China (Grant no. BZ2014034). NR 33 TC 3 Z9 3 U1 7 U2 81 PU ELSEVIER ADVANCED TECHNOLOGY PI OXFORD PA OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-5663 EI 1873-4235 J9 BIOSENS BIOELECTRON JI Biosens. Bioelectron. PD JUN 15 PY 2015 VL 68 BP 516 EP 520 DI 10.1016/j.bios.2015.01.028 PG 5 WC Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology SC Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics GA CD7CL UT WOS:000351248300073 PM 25636024 ER PT J AU Rasooly, R Balsam, J Hernlem, BJ Rasooly, A AF Rasooly, Reuven Balsam, Josh Hernlem, Bradley J. Rasooly, Avraham TI Sensitive detection of active Shiga toxin using low cost CCD based optical detector SO BIOSENSORS & BIOELECTRONICS LA English DT Article DE Food; Shiga toxin; CCD camera; Adenovirus ID LINKED-IMMUNOSORBENT-ASSAY; ESCHERICHIA-COLI O157-H7; STAPHYLOCOCCAL-ENTEROTOXIN; UNITED-STATES; FOOD; PCR AB To reduce the sources and incidence of food-borne illness there is a need to develop affordable, sensitive devices for detection of active toxins, such as Shiga toxin type 2 (Stx2). Currently the widely used methods for measuring Shiga toxin are immunoassay that cannot distinguish between the active form of the toxin, which poses a threat to life, to the inactive form which can bind to antibodies but show no toxicity. In this work, we determine toxin activity based on Shiga toxin inhibition of green fluorescent protein (GFP) combined with low cost charge-coupled device (CCD) fluorescence detection, which is more clinically relevant than immunoassay. For assay detection, a simple low cost fluorescence detection system was constructed using a CCD camera and light emitting diode (LED) excitation source, to measure GFP expression. The system was evaluated and compared to a commercial fluorometer using photo-multiplier detection for detecting active Stx2 in the range 100 ng/mL-0.01 pg/mL. The result shows that there is a negative linear relationship between Stx2 concentrations and luminous intensity of GFP, imaged by the CCD camera (R-2=0.85) or fluorometer (R-2=0.86). The low cost (similar to$300) CCD camera is capable of detecting Shiga toxin activity at comparable levels as a more expensive (similar to$30,000) fluorometer. These results demonstrate the utility and the potential of low cost detectors for toxin activity; this approach may increase the availability of foodborne bacterial toxin diagnostics in regions where there are limited resources and could be readily adapted to the detection of other food-borne toxins. Published by Elsevier B.V. C1 [Rasooly, Reuven; Hernlem, Bradley J.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Balsam, Josh; Rasooly, Avraham] US FDA, Div Biol, Off Sci & Engn, Silver Spring, MD 20993 USA. [Balsam, Josh] Univ Maryland, College Pk, MD 20742 USA. [Rasooly, Avraham] NCI, Off Canc Complementary & Alternat Med, Rockville, MD 20850 USA. RP Rasooly, R (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov NR 20 TC 3 Z9 3 U1 4 U2 46 PU ELSEVIER ADVANCED TECHNOLOGY PI OXFORD PA OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-5663 EI 1873-4235 J9 BIOSENS BIOELECTRON JI Biosens. Bioelectron. PD JUN 15 PY 2015 VL 68 BP 705 EP 711 DI 10.1016/j.bios.2015.01.065 PG 7 WC Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology SC Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics GA CD7CL UT WOS:000351248300100 PM 25677808 ER PT J AU McMahan, C Kostyal, D Lhamo, D Cornish, K AF McMahan, Colleen Kostyal, David Lhamo, Dhondup Cornish, Katrina TI Protein influences on guayule and Hevea natural rubber sol and gel SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE biomaterials; biopolymers and renewable polymers; gels; proteins; rubber ID STRUCTURAL-CHARACTERIZATION; LATEX; DEGRADATION AB Guayule (Parthenium argentatum) is under cultivation in the southwestern United States as an alternative source of natural rubber free from proteins that cause Type I latex allergies. However, since guayule lacks the protein-polymer interactions present in Hevea latex, its physical and chemical properties may differ. The solvent-soluble (Sol) and insoluble (Gel) fractions from guayule and Hevea natural rubbers were isolated through a solubilization/centrifugation deproteinization process. Protein could be reduced or removed by centrifugation, or concentrated in the gel fraction for both Hevea and guayule rubber. Separation of the sol fraction of Hevea rubber reduced the overall protein level, in some cases to below detection limits, without impacting rubber thermo-oxidative stability. Notably, no detectable cross reactions took place between guayule protein antibodies and Hevea-based materials, nor vice-versa. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42051. C1 [McMahan, Colleen; Lhamo, Dhondup] ARS, USDA, Western Reg Res Lab, Albany, CA 94710 USA. [Kostyal, David] Akron Rubber Dev Lab, Dept Microbiol, Akron, OH 44305 USA. [Cornish, Katrina] Ohio State Univ, Dept Hort & Crop Sci, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Cornish, Katrina] Ohio State Univ, Dept Food Agr & Biol Engn, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. RP McMahan, C (reprint author), ARS, USDA, Western Reg Res Lab, Albany, CA 94710 USA. EM colleen.mcmahan@ars.usda.gov RI Cornish, Katrina/A-9773-2013 FU USDA National Institute of Food and Agriculture, Hatch project [230837] FX Technical support from Dr. Wenshuang Xie, Mr. Richard Kamenik, Dr. Upul Hathwaik, and Ms. Sharette Colbert is gratefully acknowledged. This work is also supported by the USDA National Institute of Food and Agriculture, Hatch project 230837. NR 25 TC 6 Z9 6 U1 2 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD JUN 15 PY 2015 VL 132 IS 23 AR 42051 DI 10.1002/app.42051 PG 7 WC Polymer Science SC Polymer Science GA CD9BY UT WOS:000351392700030 ER PT J AU Krishnan, HB Wang, TTY AF Krishnan, Hari B. Wang, Thomas T. Y. TI An effective and simple procedure to isolate abundant quantities of biologically active chemopreventive Lunasin Protease Inhibitor Concentrate (LPIC) from soybean SO FOOD CHEMISTRY LA English DT Article DE Bowman-Birk inhibitor; Chemopreventive agent; Lunasin; LPIC (Lunasin Protease Inhibitor Concentrate) ID BOWMAN-BIRK INHIBITOR; BREAST-CANCER RISK; SOY PROTEIN; GLYCINE-MAX; PEPTIDE; PRODUCTS; AGENT; MACROPHAGES; EXPRESSION; PREVENTION AB Lunasin is a 5-kDa soybean bioactive peptide with demonstrated anti-cancer and anti-inflammatory properties. Recently, purification methods have been developed to obtain gram quantities of lunasin. However, these methods are cumbersome, time consuming and cost-prohibitive. To overcome these constrains we have developed a novel method which involves extraction of soybean flour with 30% ethanol followed by preferential precipitation of lunasin and protease inhibitors by calcium. The calcium precipitated protein fraction, which we termed as Lunasin Protease Inhibitor Concentrate (LPIC), contains three abundant proteins with molecular weights of 21,14 and 5 kDa. This simple procedure yields 3.2 g of LPIC from 100 g of soybean flour and the entire isolation procedure can be completed in less than 2 h. Treatment of THP-1 human monocyte cell lines with LPIC resulted in suppression of lipopolysaccharide-stimulated cytokine expression, demonstrating that the LPIC isolated by our simple procedure is biologically active. Published by Elsevier Ltd. C1 [Krishnan, Hari B.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. [Krishnan, Hari B.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. [Wang, Thomas T. Y.] ARS, Diet Gen & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Krishnan, HB (reprint author), Univ Missouri, USDA ARS, 108 Curtis Hall, Columbia, MO 65211 USA. EM Hari.Krishnan@ARS.USDA.GOV FU ARS Projects [3622-21000-000-00D, 1235-51530-053-00D] FX The authors thank Nathan Oehrle (USDA-ARS, University of Missouri) for technical assistance. Funding for this research was provided by ARS Projects 3622-21000-000-00D (H.B.K.) and 1235-51530-053-00D (T.W.). Product names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may be suitable. NR 38 TC 2 Z9 2 U1 4 U2 52 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JUN 15 PY 2015 VL 177 BP 120 EP 126 DI 10.1016/j.foodchem.2015.01.006 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CC1IC UT WOS:000350093400017 PM 25660866 ER PT J AU Kinney, KM Asner, GP Cordell, S Chadwick, OA Heckman, K Hotchkiss, S Jeraj, M Kennedy-Bowdoin, T Knapp, DE Questad, EJ Thaxton, JM Trusdell, F Kellner, JR AF Kinney, Kealohanuiopuna M. Asner, Gregory P. Cordell, Susan Chadwick, Oliver A. Heckman, Katherine Hotchkiss, Sara Jeraj, Marjeta Kennedy-Bowdoin, Ty Knapp, David E. Questad, Erin J. Thaxton, Jarrod M. Trusdell, Frank Kellner, James R. TI Primary Succession on a Hawaiian Dryland Chronosequence SO PLOS ONE LA English DT Article ID FOREST SUCCESSION; SOIL; ECOSYSTEMS; PHOSPHORUS; DYNAMICS; MATRIX; FIRE; AMS AB We used measurements from airborne imaging spectroscopy and LiDAR to quantify the biophysical structure and composition of vegetation on a dryland substrate age gradient in Hawaii. Both vertical stature and species composition changed during primary succession, and reveal a progressive increase in vertical stature on younger substrates followed by a collapse on Pleistocene-aged flows. Tall-stature Metrosideros polymorpha woodlands dominated on the youngest substrates (hundreds of years), and were replaced by the tall-stature endemic tree species Myoporum sandwicense and Sophora chrysophylla on intermediate-aged flows (thousands of years). The oldest substrates (tens of thousands of years) were dominated by the short-stature native shrub Dodonaea viscosa and endemic grass Eragrostis atropioides. We excavated 18 macroscopic charcoal fragments from Pleistocene-aged substrates. Mean radiocarbon age was 2,002 years and ranged from < 200 to 7,730. Genus identities from four fragments indicate that Osteomeles spp. or M. polymorpha once occupied the Pleistocene-aged substrates, but neither of these species is found there today. These findings indicate the existence of fires before humans are known to have occupied the Hawaiian archipelago, and demonstrate that a collapse in vertical stature is prevalent on the oldest substrates. This work contributes to our understanding of prehistoric fires in shaping the trajectory of primary succession in Hawaiian drylands. C1 [Kinney, Kealohanuiopuna M.; Kellner, James R.] Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA. [Kinney, Kealohanuiopuna M.; Cordell, Susan] US Forest Serv, USDA, Inst Pacific Isl Forestry, Hilo, HI USA. [Asner, Gregory P.; Kennedy-Bowdoin, Ty; Knapp, David E.] Carnegie Inst Sci, Dept Global Ecol, Stanford, CA USA. [Chadwick, Oliver A.] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Heckman, Katherine] Lawrence Livermore Natl Lab, USDA, Forest Serv, Northern Res Stn,Ctr Accelerator Mass Spectrometr, Livermore, CA USA. [Hotchkiss, Sara; Jeraj, Marjeta] Univ Wisconsin, Dept Bot, Madison, WI USA. [Questad, Erin J.] Calif Polytech Univ, Dept Biol Sci, Pomona, CA 91768 USA. [Thaxton, Jarrod M.] Eastern Kentucky Univ, Dept Biol Sci, Richmond, KY 40475 USA. [Trusdell, Frank] US Geol Survey, Hawaii Volcano Observ, Volcano, HI USA. RP Kinney, KM (reprint author), Brown Univ, Dept Ecol & Evolutionary Biol, Providence, RI 02912 USA. EM kealohanuiopuna_kinney@brown.edu OI Hotchkiss, Sara/0000-0002-0383-0144 FU USDA Forest Service Pacific Southwest Research Station; University of Maryland, College Park; Strategic Environmental Research and Development Program FX This work was supported by a Joint Venture Agreement between the USDA Forest Service Pacific Southwest Research Station and the University of Maryland, College Park, and by a grant from the Strategic Environmental Research and Development Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 27 TC 0 Z9 0 U1 14 U2 35 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 12 PY 2015 VL 10 IS 6 AR e0123995 DI 10.1371/journal.pone.0123995 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6GR UT WOS:000356327000001 PM 26066334 ER PT J AU Mahmoud, MS Kandil, OM Nasr, SM Hendawy, SHM Habeeb, SM Mabrouk, DM Silva, MG Suarez, CE AF Mahmoud, Mona S. Kandil, Omnia M. Nasr, Soad M. Hendawy, Seham H. M. Habeeb, Salwa M. Mabrouk, Dalia M. Silva, Marta G. Suarez, Carlos E. TI Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt SO PARASITES & VECTORS LA English DT Article DE B. bigemina; Babesia bovis; Competitive ELISA; Nested PCR; Semi-nested PCR; Hemogram; Egypt ID LINKED-IMMUNOSORBENT-ASSAY; CHAIN-REACTION AMPLIFICATION; WATER-BUFFALOS; CARRIER CATTLE; BOVINE BLOOD; BIGEMINA; ANTIBODIES; VALIDATION; DISEASES; VETERINARY AB Background: Babesiosis threatens the development of the cattle and buffaloes industries in Egypt and improved control is needed. The main objectives of this study are surveying the presence of bovine babesiosis in distinct selected bovine and buffalo populations in Egypt using novel molecular and previously validated serological methods, while also comparing the occurrence of hematological alterations among Babesia infected cattle and buffalos. Methods: A total of 253 and 81 blood samples from apparently healthy cattle and buffaloes, respectively, were randomly collected from diverse locations in Egypt. All samples were tested for Babesia bovis and B. bigemina infection using blood film examination, competitive ELISA (cELISA) and PCR. Novel semi-nested and nested PCR assays for the detection of Babesia bovis and B. bigemina respectively, were developed and used to analyze DNA extracted from bovine and buffalo samples. Hematological profiles were studied using a hematological analyzer. Results: Blood films examination revealed 13.8 % and 7.4 % Babesia infection rates in cattle and buffaloes, respectively. However, in cattle, the cELISA detected 32.8 %, 21.3 % and 10.7 % infection rates with B. bigemina, Babesia bovis and mixed infection, respectively. In addition, cELISA identified 22.2 %, 22.2 % and 6.2 % infection rates with B. bigemina, Babesia bovis and mixed infection, respectively in buffaloes. The semi-nested PCR assay showed that 15 % of the tested samples were positive for Babesia bovis in cattle, but just 3 % in buffaloes. Infections with B. bigemina were also found in cattle (32.4 %), but not in buffaloes upon nested PCR analysis. Sequencing analysis confirmed the identity of the PCR amplicons and showed that Egyptian genotypes of B. bigemina and Babesia bovis highly resemble sequences previously deposited in GenBank. Hemograms performed on the sampled animals revealed macrocytic hypochromic anemia associated with reduced platelet counts in infected cattle with babesiosis. In addition, marked increases in total leukocyte and granulocytic counts and decreases in lymphocytic counts were found in infected cattle. In contrast, no such hematological anomalies were found in presumably Babesia-infected buffaloes. Conclusions: Frequent occurrence of babesiosis among apparently healthy bovines in Egypt, suggests the need for appropriately designed prevalence studies in this country. Infected bovine, but not buffalo, populations often present hematological disorders compatible with intravascular hemolysis and thrombocytopenia. C1 [Mahmoud, Mona S.; Kandil, Omnia M.; Nasr, Soad M.; Hendawy, Seham H. M.; Habeeb, Salwa M.] Natl Res Ctr, Parasitol & Anim Dis Dept, Dokki 12622, Egypt. [Mabrouk, Dalia M.] Natl Res Ctr, Dept Cell Biol, Dokki 12622, Egypt. [Silva, Marta G.; Suarez, Carlos E.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Suarez, Carlos E.] USDA, Agr Res Serv, Anim Dis Res Unit, Pullman, WA USA. RP Silva, MG (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM marta@vetmed.wsu.edu OI Hendawy, Seham/0000-0001-7122-1570; Mahmoud, Mona/0000-0001-5017-9182; Habeeb, Salwa/0000-0001-9431-5211; Kandil, Omnia/0000-0001-7849-2955; Nasr, Soad/0000-0002-1538-8912 FU USAID through STDF [3866] FX This work was supported by the USAID Grant 3866 funded through the STDF. We are thankful to Dr. Chungwon Chung and Dr. Scott Adams from VMRD (Pullman, WA) for kindly providing the cELISA kits used for the serological diagnosis of bovine and buffalo babesiosis, to Paul Lacy for technical help in the development of the PCR reactions and to Jacob Laughery for technical help. We also would like to thank Dr. Don Knowles for facilitating the development of this project. NR 55 TC 5 Z9 6 U1 0 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD JUN 12 PY 2015 VL 8 AR 319 DI 10.1186/s13071-015-0928-9 PG 15 WC Parasitology SC Parasitology GA CK4TL UT WOS:000356216000001 PM 26062684 ER PT J AU Kistler, WM Stallknecht, DE DeLiberto, TJ Van Why, K Yabsley, MJ AF Kistler, Whitney M. Stallknecht, David E. DeLiberto, Thomas J. Van Why, Kyle Yabsley, Michael J. TI Subtype-specific influenza A virus antibodies in Canada geese (Branta canadensis) SO VETERINARY MICROBIOLOGY LA English DT Article DE Canada geese; Hemagglutination inhibition; Influenza A virus; Sentinel; Serology ID NORTH-AMERICA; WILD BIRDS; SURVEILLANCE; CHALLENGE; WATERFOWL; PATTERNS; DUCKS; H5N2 AB Historically, surveillance for influenza A viruses (IAVs) in wild birds has relied on viral detection assays. This was largely due to poor performance of serological assays in wild birds; however, recently developed commercial serological assays have improved the ability to detect IAV antibodies in wild birds. Serological surveillance for IAV antibodies in Canada geese (Branta canadensis) has shown that, despite a low prevalence of virus isolations, Canada geese are frequently exposed to IAVs and that exposure increases with latitude, which follows virus isolation prevalence patterns observed in dabbling ducks. The objectives of this study were to further evaluate IAV antibodies in Canada geese using a subtype-specific serological assay to determine if Canada geese are exposed to subtypes that commonly circulate in dabbling ducks. We collected serum samples from Canada geese in Minnesota, New Jersey, Pennsylvania, and Wisconsin and tested for antibodies to IAVs using a blocking ELISA. Positive samples were further tested by hemagglutination inhibition for 10 hemagglutinin IAV subtypes (H1-H10). Overall, we detected antibodies to NP in 24% (714/2919) of geese. Antibodies to H3, H4, H5, and H6 subtypes predominated, with H5 being detected most frequently. A decrease in H5 HI antibody prevalence and titers was observed from 2009 to 2012. We also detected similar exposure pattern in Canada geese from New Jersey, Minnesota, Washington and Wisconsin. Based on the published literature, H3, H4, and H6 viruses are the most commonly reported IAVs from dabbling ducks. These results indicate that Canada geese also are frequently exposed to viruses of the same HA subtypes; however, the high prevalence of antibodies to H5 viruses was not expected as H5 IAVs are generally not well represented in reported isolates from ducks. (C) 2015 Elsevier B.V. All rights reserved. C1 [Kistler, Whitney M.; Stallknecht, David E.; Yabsley, Michael J.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Kistler, Whitney M.; Yabsley, Michael J.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [DeLiberto, Thomas J.; Van Why, Kyle] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Kistler, WM (reprint author), Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. EM whitney.kistler@gmail.com FU Department of Homeland Security, Center of Excellence for Emerging and Zoonotic animal Diseases [2010-ST-061-AG0001-02]; National Institute of Allergy and Infectious Diseases (NIAAD), National Institutes of Health (NIH), Department of Health and Human Services [HHSN266200700007C] FX We thank J. Parris, J. Slusher, and A. Howey-Newsome (Southeastern Cooperative Wildlife Disease Study) and personal at the Pennsylvania Game Commission, United States Department of Agriculture Wildlife Services, New Jersey Department of Natural Resources, Minnesota Department of Natural Resources, and Wisconsin Department of Natural Resources for field assistance. Funding was provided by Department of Homeland Security, Center of Excellence for Emerging and Zoonotic animal Diseases cooperative agreement 2010-ST-061-AG0001-02 and contract HHSN266200700007C from National Institute of Allergy and Infectious Diseases (NIAAD), National Institutes of Health (NIH), Department of Health and Human Services. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 23 TC 0 Z9 0 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD JUN 12 PY 2015 VL 177 IS 3-4 BP 296 EP 301 DI 10.1016/j.vetmic.2015.03.021 PG 6 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CJ3AR UT WOS:000355356300008 PM 25845755 ER PT J AU Silver, MJ Kessler, NJ Hennig, BJ Dominguez-Salas, P Laritsky, E Baker, MS Coarfa, C Hernandez-Vargas, H Castelino, JM Routledge, MN Gong, YY Herceg, Z Lee, YS Lee, K Moore, SE Fulford, AJ Prentice, AM Waterland, RA AF Silver, Matt J. Kessler, Noah J. Hennig, Branwen J. Dominguez-Salas, Paula Laritsky, Eleonora Baker, Maria S. Coarfa, Cristian Hernandez-Vargas, Hector Castelino, Jovita M. Routledge, Michael N. Gong, Yun Yun Herceg, Zdenko Lee, Yong Sun Lee, Kwanbok Moore, Sophie E. Fulford, Anthony J. Prentice, Andrew M. Waterland, Robert A. TI Independent genomewide screens identify the tumor suppressor VTRNA2-1 as a human epiallele responsive to periconceptional environment SO GENOME BIOLOGY LA English DT Article ID EPIGENOME-WIDE ASSOCIATION; DNA METHYLATION ARRAY; EPIGENETIC EPIDEMIOLOGY; METASTABLE EPIALLELES; TRANSPOSABLE ELEMENTS; GENE; ILLUMINA; CANCER; MECHANISMS; DISEASES AB Background: Interindividual epigenetic variation that occurs systemically must be established prior to gastrulation in the very early embryo and, because it is systemic, can be assessed in easily biopsiable tissues. We employ two independent genome-wide approaches to search for such variants. Results: First, we screen for metastable epialleles by performing genomewide bisulfite sequencing in peripheral blood lymphocyte (PBL) and hair follicle DNA from two Caucasian adults. Second, we conduct a genomewide screen for genomic regions at which PBL DNA methylation is affected by season of conception in rural Gambia. Remarkably, both approaches identify the genomically imprinted VTRNA2-1 as a top environmentally responsive epiallele. We demonstrate systemic and stochastic interindividual variation in DNA methylation at the VTRNA2-1 differentially methylated region in healthy Caucasian and Asian adults and show, in rural Gambians, that periconceptional environment affects offspring VTRNA2-1 epigenotype, which is stable over at least 10 years. This unbiased screen also identifies over 100 additional candidate metastable epialleles, and shows that these are associated with cis genomic features including transposable elements. Conclusions: The non-coding VTRNA2-1 transcript (also called nc886) is a putative tumor suppressor and modulator of innate immunity. Thus, these data indicating environmentally induced loss of imprinting at VTRNA2-1 constitute a plausible causal pathway linking early embryonic environment, epigenetic alteration, and human disease. More broadly, the list of candidate metastable epialleles provides a resource for future studies of epigenetic variation and human disease. C1 [Silver, Matt J.; Hennig, Branwen J.; Moore, Sophie E.; Fulford, Anthony J.; Prentice, Andrew M.] London Sch Hyg & Trop Med, MRC, Int Nutr Grp, London WC1E 7HT, England. [Silver, Matt J.; Hennig, Branwen J.; Moore, Sophie E.; Fulford, Anthony J.; Prentice, Andrew M.] MRC Keneba, MRC Unit, Banjul, Gambia. [Kessler, Noah J.; Laritsky, Eleonora; Baker, Maria S.; Waterland, Robert A.] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Dominguez-Salas, Paula] Royal Vet Coll, Vet Epidemiol Econ & Publ Hlth Grp, Hatfield AL9 7TA, Herts, England. [Dominguez-Salas, Paula] Int Livestock Res Inst, Nairobi 00100, Kenya. [Coarfa, Cristian] Baylor Coll Med, Dept Mol & Cell Biol, Houston, TX 77030 USA. [Hernandez-Vargas, Hector; Herceg, Zdenko] IARC, Epigenet Grp, F-69372 Lyon 08, France. [Castelino, Jovita M.; Routledge, Michael N.] Univ Leeds, Sch Med, Leeds LS2 9JT, W Yorkshire, England. [Gong, Yun Yun] Queens Univ Belfast, Inst Global Food Secur, Belfast BT9 5AG, Antrim, North Ireland. [Lee, Yong Sun; Lee, Kwanbok] Univ Texas Med Branch, Dept Biochem & Mol Biol, Galveston, TX 77555 USA. [Moore, Sophie E.] MRC Human Nutr Res, Elsie Widdowson Lab, Cambridge CB1 9NL, England. [Waterland, Robert A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. RP Prentice, AM (reprint author), London Sch Hyg & Trop Med, MRC, Int Nutr Grp, Keppel St, London WC1E 7HT, England. EM Andrew.Prentice@lshtm.ac.uk; waterland@bcm.edu RI Gong, Yun Yun/L-7094-2016; OI Gong, Yun Yun/0000-0003-4927-5526; Silver, Matt/0000-0002-3852-9677 FU NIH/National Institute of Diabetes and Digestive and Kidney Diseases [1R01DK081557]; USDA [CRIS 6250-51000-055]; Wellcome Trust [WT086369MA]; International Nutrition Group by the UK Medical Research Council (MRC) [MCA760-5QX00]; UK Department for International Development (DFID) under the MRC/DFID Concordat agreement; IARC; Grand Challenges Exploration grant from the Bill and Melinda Gates Foundation FX We thank Adam Gillum (Baylor College of Medicine) for assistance with the figures, Dr Yongtao Guan (Baylor College of Medicine) for help with the analysis of the LCL methylation data, and Dr Florence Le Calvez-Kelm and Mr Geoffroy Durand from the Genetic Cancer Susceptibility group (IARC, Lyon) for performing the HM450 beadchip assays. RAW was supported by grants from NIH/National Institute of Diabetes and Digestive and Kidney Diseases (1R01DK081557) and the USDA (CRIS 6250-51000-055). The Gambian study was supported by a Wellcome Trust grant WT086369MA (to BJH), and core funding MCA760-5QX00 to the International Nutrition Group by the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement. Our thanks go to the study participants in The Gambia and staff at MRC Keneba, The Gambia. MNR, JMC and YYG were supported by a grant from IARC. HHV and ZH were supported by a Grand Challenges Exploration grant from the Bill and Melinda Gates Foundation. NR 46 TC 24 Z9 24 U1 1 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1465-6906 EI 1474-760X J9 GENOME BIOL JI Genome Biol. PD JUN 11 PY 2015 VL 16 AR 118 DI 10.1186/s13059-015-0660-y PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CK0OF UT WOS:000355903800001 PM 26062908 ER PT J AU Bellaloui, N Bruns, HA Abbas, HK Mengistu, A Fisher, DK Reddy, KN AF Bellaloui, Nacer Bruns, Herbert A. Abbas, Hamed K. Mengistu, Alemu Fisher, Daniel K. Reddy, Krishna N. TI Effects of Row-Type, Row-Spacing, Seeding Rate, Soil-Type, and Cultivar Differences on Soybean Seed Nutrition under US Mississippi Delta Conditions SO PLOS ONE LA English DT Article ID AZOMETHINE-H METHOD; RADIATION INTERCEPTION; CHEMICAL-COMPOSITION; PRODUCTION SYSTEM; SOLAR-RADIATION; NARROW ROWS; SINGLE-ROW; YIELD; MATURITY; DROUGHT AB The new Early Soybean Production System (ESPS), developed in the Midsouth USA, including the Mississippi delta, resulted in higher yield under irrigated and non-irrigated conditions. However, information on the effects of the agricultural practices such as row-type (RT: twin-vs. single-row), row-spacing, (RS), seeding rate (SR), soil-type (ST) on seed nutrition under the ESPS environment in the Mississippi delta is very limited. Our previous research in the Mississippi delta showed these agricultural practices altered seed nutrients in one cultivar only. However, whether these effects on seed nutrients will be exhibited by other soybean cultivars with earlier and later maturities across multiple years are not yet known. Therefore, the objective of this research was to evaluate the effects of agricultural practices and cultivar (Cv) differences on seed nutrition in clay and sandy soils under ESPS environment of high heat and drought. Two field experiments were conducted; one experiment was conducted in 2009 and 2010, and the other in 2008, 2009, and 2010 under irrigated conditions. Soybean were grown on 102 cm single-rows and on 25 cmtwin-rows with 102 cm centers at seeding rates of 20, 30, 40, and 50 seeds m-2. Two soybean cultivars (94M80 with earlier maturity; and GP 533 with later maturity) were used. Results showed that increasing seeding rate resulted in increases of protein, sucrose, glucose, raffinose, B, and P concentrations on both single-and twin-rows. However, this increase became either constant or declined at the higher rates (40 and 50 seeds m(-2)). Protein and linolenic acid concentrations were higher in GP 533 than in 94M80 on both row-types, but oil and oleic acid concentrations were in 94M80 than GP 533. Generally, cultivar GP 533 accumulated more seed constituents in seeds than 94M80. In 2010, there were no clear responses of seed nutrients to SR increase in both cultivars, perhaps due to drier year and high heat in 2010. It is concluded that RT and SR can alter seed nutrition under clay and sandy soils, especially under high heat and drought conditions as in the Mississippi delta. C1 [Bellaloui, Nacer] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Bruns, Herbert A.; Fisher, Daniel K.; Reddy, Krishna N.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Abbas, Hamed K.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Mengistu, Alemu] USDA ARS, Crop Genet Res Unit, Jackson, TN 38301 USA. RP Bellaloui, N (reprint author), USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM nacer.bellaloui@ars.usda.gov FU USDA-ARS (US federal agency); Agricultural Research Service Project [6402-21220-012-00D] FX This study was funded by USDA-ARS (US federal agency). The grant number is: Agricultural Research Service Project 6402-21220-012-00D. Website: http://www.ars.usda.gov/pandp/people/people.htm?personid=37050. NR 53 TC 1 Z9 1 U1 2 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0129913 DI 10.1371/journal.pone.0129913 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500166 PM 26061182 ER PT J AU Ferreira, AM Marques, AT Bhide, M Cubric-Curik, V Hollung, K Knight, CH Raundrup, K Lippolis, J Palmer, M Sales-Baptista, E Araujo, SS de Almeida, AM AF Ferreira, Ana Monteiro Marques, Andreia Tomas Bhide, Mangesh Cubric-Curik, Vlatka Hollung, Kristin Knight, Christopher Harold Raundrup, Katrine Lippolis, John Palmer, Mitchell Sales-Baptista, Elvira Araujo, Susana Sousa de Almeida, Andre Martinho TI Sequence Analysis of Bitter Taste Receptor Gene Repertoires in Different Ruminant Species SO PLOS ONE LA English DT Article ID EVOLUTION; IDENTIFICATION; VERTEBRATES; FAMILIES; HUMANS AB Bitter taste has been extensively studied in mammalian species and is associated with sensitivity to toxins and with food choices that avoid dangerous substances in the diet. At the molecular level, bitter compounds are sensed by bitter taste receptor proteins (T2R) present at the surface of taste receptor cells in the gustatory papillae. Our work aims at exploring the phylogenetic relationships of T2R gene sequences within different ruminant species. To accomplish this goal, we gathered a collection of ruminant species with different feeding behaviors and for which no genome data is available: American bison, chamois, elk, European bison, fallow deer, goat, moose, mouflon, muskox, red deer, reindeer and white tailed deer. The herbivores chosen for this study belong to different taxonomic families and habitats, and hence, exhibit distinct foraging behaviors and diet preferences. We describe the first partial repertoires of T2R gene sequences for these species obtained by direct sequencing. We then consider the homology and evolutionary history of these receptors within this ruminant group, and whether it relates to feeding type classification, using MEGA software. Our results suggest that phylogenetic proximity of T2R genes corresponds more to the traditional taxonomic groups of the species rather than reflecting a categorization by feeding strategy. C1 [Ferreira, Ana Monteiro; Sales-Baptista, Elvira] Univ Evora, ICAAM, P-7006554 Evora, Portugal. [Ferreira, Ana Monteiro; Araujo, Susana Sousa; de Almeida, Andre Martinho] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier ITQB, Plant Cell Biotechnol Lab, P-2780157 Oeiras, Portugal. [Marques, Andreia Tomas; Araujo, Susana Sousa; de Almeida, Andre Martinho] Inst Invest Cient Trop, P-1300344 Lisbon, Portugal. [Bhide, Mangesh] Univ Vet & Pharm, Lab Biomed Microbiol & Immunol, Kosice 04181, Slovakia. [Cubric-Curik, Vlatka] Univ Zagreb, Fac Agr, Dept Anim Sci, Zagreb 10000, Croatia. [Hollung, Kristin] Norwegian Food Res Inst, NOFIMA, N-1430 As, Norway. [Knight, Christopher Harold] Univ Copenhagen, Fac Hlth & Med Sci, DK-2200 Copenhagen, Denmark. [Raundrup, Katrine] Greenland Inst Nat Resources, Nuuk 3900, Greenland. [Lippolis, John] USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. [Palmer, Mitchell] USDA, Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA. [Sales-Baptista, Elvira] Univ Evora, Dept Zootecnia, P-7002554 Evora, Portugal. [de Almeida, Andre Martinho] CIISA, P-1300477 Lisbon, Portugal. [de Almeida, Andre Martinho] IBET, P-2780157 Oeiras, Portugal. RP de Almeida, AM (reprint author), Ross Univ, Sch Vet Med, PO 334, Basseterre, St Kitts & Nevi. EM adealmeida@rossvet.edu.kn RI Araujo, Susana/A-5482-2009; CIISA, FMV/D-2242-2016; Almeida, Andre/I-4369-2013; OI Araujo, Susana/0000-0003-2823-088X; Almeida, Andre/0000-0002-7810-3988; Sales-Baptista, Elvira/0000-0002-6318-4498 FU Fundacao para a Ciencia e a Tecnologia (Lisboa, Portugal) [SFRH/BPD/69655/2010, SFRH/BPD/90916/2012]; Ciencia program FX The authors acknowledge financial support from Fundacao para a Ciencia e a Tecnologia (Lisboa, Portugal) in the form of the grants SFRH/BPD/69655/2010 (A. M. Ferreira) and SFRH/BPD/90916/2012 (A. M. de Almeida) and Research Contract by the Ciencia 2008 program (S. S. Araujo). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 34 TC 1 Z9 1 U1 7 U2 37 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0124933 DI 10.1371/journal.pone.0124933 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500008 ER PT J AU Guan, W Shao, J Elbeaino, T Davis, RE Zhao, TC Huang, Q AF Guan, Wei Shao, Jonathan Elbeaino, Toufic Davis, Robert E. Zhao, Tingchang Huang, Qi TI Specific Detection and Identification of American Mulberry-Infecting and Italian Olive-Associated Strains of Xylella fastidiosa by Polymerase Chain Reaction SO PLOS ONE LA English DT Article ID BACTERIAL LEAF SCORCH; CITRUS VARIEGATED CHLOROSIS; SOUTHERN CALIFORNIA; PIERCES-DISEASE; OLEANDER; HOST; TREES; QUANTIFICATION; GRAPEVINE; ALMOND AB Xylella fastidiosa causes bacterial leaf scorch in many landscape trees including elm, oak, sycamore and mulberry, but methods for specific identification of a particular tree host species- limited strain or differentiation of tree-specific strains are lacking. It is also unknown whether a particular landscape tree-infecting X. fastidiosa strain is capable of infecting multiple landscape tree species in an urban environment. We developed two PCR primers specific for mulberry-infecting strains of X. fastidiosa based on the nucleotide sequence of a unique open reading frame identified only in mulberry-infecting strains among all the North and South American strains of X. fastidiosa sequenced to date. PCR using the primers allowed for detection and identification of mulberry-infecting X. fastidiosa strains in cultures and in samples collected from naturally infected mulberry trees. In addition, no mixed infections with or non-specific detections of the mulberry-infecting strains of X. fastidiosa were found in naturally X. fastidiosa-infected oak, elm and sycamore trees growing in the same region where naturally infected mulberry trees were grown. This genotype-specific PCR assay will be valuable for disease diagnosis, studies of strain-specific infections in insects and plant hosts, and management of diseases caused by X. fastidiosa. Unexpectedly but interestingly, the unique open reading frame conserved in the mulberry-infecting strains in the U.S. was also identified in the recently sequenced olive-associated strain CoDiRO isolated in Italy. When the primer set was tested against naturally infected olive plant samples collected in Italy, it allowed for detection of olive-associated strains of X. fastidiosa in Italy. This PCR assay, therefore, will also be useful for detection and identification of the Italian group of X. fastidiosa strains to aid understanding of the occurrence, evolution and biology of this new group of X. fastidiosa strains. C1 [Guan, Wei; Zhao, Tingchang] Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China. [Guan, Wei; Huang, Qi] USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. [Shao, Jonathan; Davis, Robert E.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. [Elbeaino, Toufic] Ist Agron Mediterraneo, Valenzano, BA, Italy. RP Huang, Q (reprint author), USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. EM qi.huang@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This research was funded by the United States Department of Agriculture, Agricultural Research Service. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 2 Z9 2 U1 5 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0129330 DI 10.1371/journal.pone.0129330 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500139 PM 26061051 ER PT J AU Lu, S Yin, XY Spollen, W Zhang, N Xu, D Schoelz, J Bilyeu, K Zhang, ZYJ AF Lu, Sha Yin, Xiaoyan Spollen, William Zhang, Ning Xu, Dong Schoelz, James Bilyeu, Kristin Zhang, Zhanyuan J. TI Analysis of the siRNA-Mediated Gene Silencing Process Targeting Three Homologous Genes Controlling Soybean Seed Oil Quality SO PLOS ONE LA English DT Article ID RNAI ENZYME COMPLEX; GUIDE STRAND; PLANTS; ARABIDOPSIS; SPECIFICITY; SUPPRESSION; SELECTION; PATHWAYS; PROTEINS; MICRORNA AB In the past decade, RNA silencing has gained significant attention because of its success in genomic scale research and also in the genetic improvement of crop plants. However, little is known about the molecular basis of siRNA processing in association with its target transcript. To reveal this process for improving hpRNA-mediated gene silencing in crop plants, the soybean GmFAD3 gene family was chosen as a test model. We analyzed RNAi mutant soybean lines in which three members of the GmFAD3 gene family were silenced. The silencing levels of FAD3A, FAD3B and FAD3C were correlated with the degrees of sequence homology between the inverted repeat of hpRNA and the GmFAD3 transcripts in the RNAi lines. Strikingly, transgenes in two of the three RNAi lines were heavily methylated, leading to a dramatic reduction of hpRNA-derived siRNAs. Small RNAs corresponding to the loop portion of the hairpin transcript were detected while much lower levels of siRNAs were found outside of the target region. siRNAs generated from the 318-bp inverted repeat were found to be diced much more frequently at stem sequences close to the loop and associated with the inferred cleavage sites on the target transcripts, manifesting "hot spots". The top candidate hpRNA-derived siRNA share certain sequence features with mature miRNA. This is the first comprehensive and detailed study revealing the siRNA-mediated gene silencing mechanism in crop plants using gene family GmFAD3 as a test model. C1 [Lu, Sha; Yin, Xiaoyan; Zhang, Zhanyuan J.] Univ Missouri, Plant Transformat Core Facil, Columbia, MO 65211 USA. [Lu, Sha; Yin, Xiaoyan; Schoelz, James; Bilyeu, Kristin; Zhang, Zhanyuan J.] Univ Missouri, Div Plant Sci, Columbia, MO USA. [Spollen, William] Univ Missouri, Bioinformat Core Facil, Columbia, MO USA. [Zhang, Ning; Xu, Dong] Univ Missouri, Dept Comp Sci, Columbia, MO USA. [Zhang, Ning; Xu, Dong] Univ Missouri, Inst Informat, Columbia, MO USA. [Bilyeu, Kristin] Univ Missouri, USDA ARS, Columbia, MO USA. RP Zhang, ZYJ (reprint author), Univ Missouri, Plant Transformat Core Facil, Columbia, MO 65211 USA. EM zhangzh@missouri.edu OI Zhang, Ning/0000-0002-1218-5044 FU China Scholarship Council [2008632140]; National Institutes of Health (NIH) [R01 GM100701]; University of Missouri; USDA-ARS Core fund; NIH FX This research was supported by 2008632140, China Scholarship Council (http://en.csc.edu.cn/). SL received the funding. An additional source of funding has been from National Institutes of Health (NIH) (R01 GM100701) (http://www.nih.gov/) and supports NZ (DX's lab). The grant funding agencies (China Scholarship Council and NIH) have no contribution to the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Except for SL and NZ, all remaining authors (XY, WS, DX, JS, ZZ) have been supported by their salary lines of the University of Missouri. KB's salary has been supported by USDA-ARS Core fund. Since these are salary support, not grants, the corresponding author hasn't described them in the fund source section. NR 35 TC 2 Z9 2 U1 1 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0129010 DI 10.1371/journal.pone.0129010 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500115 PM 26061033 ER PT J AU Machtinger, ET Geden, CJ Leppla, NC AF Machtinger, Erika T. Geden, Christopher J. Leppla, Norman C. TI Linear Dispersal of the Filth Fly Parasitoid Spalangia cameroni (Hymenoptera: Pteromalidae) and Parasitism of Hosts at Increasing Distances SO PLOS ONE LA English DT Article ID STABLE FLIES DIPTERA; STOMOXYS-CALCITRANS L; HOUSE-FLIES; MUSCIDIFURAX-RAPTORELLUS; MUSCA-DOMESTICA; BIOLOGICAL-CONTROL; BEEF-CATTLE; INUNDATIVE RELEASES; POULTRY HOUSES; MASS RELEASES AB Release of parasitic wasps (Hymenoptera: Pteromalidae) as biological control agents for house flies and stable flies in livestock confinements has had variable success. In part, this may reflect a lack of knowledge regarding the optimal distance to be used between parasitoid release stations. In the current study, we assessed the effect of linear distance on host parasitism by the wasp Spalangia cameroni Perkins. In open fields at distances ranging from 1 m to 60 m from a central point, house fly puparia were placed in a mixture of pine shavings soiled with equine manure, urine, and alfalfa hay. Releases of S. cameroni then were made using a 5: 1 host: parasitoid ratio. Host pupae were parasitized at all distances, with the highest rate of total parasitism (68.9%) recorded <= 5 m from the release site. Analyses of results using non-linear and linear models suggest that S. cameroni should be released in close proximity to host development areas. Additionally, releases may not be suitable in pasture situations where long-distance flight is required for control. However, further testing is needed to examine the effect of density-dependent dispersal and diffusion of S. cameroni. C1 [Machtinger, Erika T.; Leppla, Norman C.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Geden, Christopher J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Machtinger, ET (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. EM irishtangerine@ufl.edu FU Sustainable Agriculture Research and Education grant [GS11-101] FX Funding for this work came from Sustainable Agriculture Research and Education grant GS11-101, http://www.sare.org/, to ETM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 44 TC 1 Z9 1 U1 3 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0129105 DI 10.1371/journal.pone.0129105 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500124 PM 26061882 ER PT J AU Stahl, RS Ellis, CK Nol, P Waters, WR Palmer, M VerCauteren, KC AF Stahl, Randal S. Ellis, Christine K. Nol, Pauline Waters, W. Ray Palmer, Mitchell VerCauteren, Kurt C. TI Fecal Volatile Organic Ccompound Profiles from White-Tailed Deer (Odocoileus virginianus) as Indicators of Mycobacterium bovis Exposure or Mycobacterium bovis Bacille Calmette-Guerin (BCG) Vaccination SO PLOS ONE LA English DT Article ID LONG-CHAIN ALCOHOLS; DIAGNOSTIC-TECHNIQUES; TUBERCULOSIS; CATTLE; MANURE; TRANSMISSION; PERSISTENCE; INFECTION; MICHIGAN; BACTERIA AB White-tailed deer (Odocoileus virginianus) serve as a reservoir for bovine tuberculosis, caused by Mycobacterium bovis, and can be a source of infection in cattle. Vaccination with M. bovis Bacille Calmette Guerin (BCG) is being considered for management of bovine tuberculosis in deer. Presently, no method exists to non-invasively monitor the presence of bovine tuberculosis in deer. In this study, volatile organic compound profiles of BCG-vaccinated and non-vaccinated deer, before and after experimental challenge with M. bovis strain 95-1315, were generated using solid phase microextraction fiber head-space sampling over suspended fecal pellets with analysis by gas chromatography/mass spectrometry. Chromatograms were processed using XCMS Online to characterize ion variation among treatment groups. The principal component scores resulting from significant (alpha = 0.05) ion responses were used to build linear discriminant analysis models. The sensitivity and specificity of these models were used to evaluate the feasibility of using this analytical approach to distinguish within group comparisons between pre- and post-M. bovis challenge: non-vaccinated male or female deer, BCG-vaccinated male deer, and the mixed gender non-vaccinated deer data. Seventeen compounds were identified in this analysis. The peak areas for these compounds were used to build a linear discriminant classification model based on principal component analysis scores to evaluate the feasibility of discriminating between fecal samples from M. bovis challenged deer, irrespective of vaccination status. The model best representing the data had a sensitivity of 78.6% and a specificity of 91.4%. The fecal head-space sampling approach presented in this pilot study provides a non-invasive method to discriminate between M. bovis challenged deer and BCG-vaccinated deer. Additionally, the technique may prove invaluable for BCG efficacy studies with free-ranging deer as well as for use as a non-invasive monitoring system for the detection of tuberculosis in captive deer and other livestock. C1 [Stahl, Randal S.; Ellis, Christine K.; VerCauteren, Kurt C.] USDA, APHIS, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Nol, Pauline] USDA APHIS, Wildlife Livestock Dis Invest Team, Vet Serv Sci Technol & Anal Serv, Ft Collins, CO USA. [Waters, W. Ray; Palmer, Mitchell] USDA ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, Ames, IA USA. RP Stahl, RS (reprint author), USDA, APHIS, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. EM randal.s.stahl@aphis.usda.gov NR 46 TC 0 Z9 0 U1 4 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0129740 DI 10.1371/journal.pone.0129740 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500162 PM 26060998 ER PT J AU Zamora, IJ Stoll, B Ethun, CG Sheikh, F Yu, L Burrin, DG Brandt, ML Olutoye, OO AF Zamora, Irving J. Stoll, Barbara Ethun, Cecilia G. Sheikh, Fariha Yu, Ling Burrin, Douglas G. Brandt, Mary L. Olutoye, Oluyinka O. TI Low Abdominal NIRS Values and Elevated Plasma Intestinal Fatty Acid-Binding Protein in a Premature Piglet Model of Necrotizing Enterocolitis SO PLOS ONE LA English DT Article ID NEAR-INFRARED SPECTROSCOPY; TISSUE OXYGENATION; EARLY-DIAGNOSIS; UNITED-STATES; PRETERM PIGS; DYSFUNCTION; MORTALITY; EPIDEMIOLOGY; NUTRITION; SEVERITY AB To identify early markers of necrotizing enterocolitis (NEC), we hypothesized that continuous abdominal near-infrared spectroscopy (A-NIRS) measurement of splanchnic tissue oxygen saturation and intermittent plasma intestinal fatty-acid binding protein (pI-FABP) measured every 6 hours can detect NEC prior to onset of clinical symptoms. Premature piglets received parenteral nutrition for 48-hours after delivery, followed by enteral feeds every three hours until death or euthanasia at 96-hours. Continuous A-NIRS, systemic oxygen saturation (SpO(2)), and heart rate were measured while monitoring for clinical signs of NEC. Blood samples obtained at 6-hour intervals were used to determine pI-FABP levels by ELISA. Piglets were classified as fulminant-NEC (f-NEC), non-fulminant-NEC (nf-NEC) and No-NEC according to severity of clinical and histologic features. Of 38 piglets, 37% (n=14) developed nf-NEC, 18% (n=7) developed f-NEC and 45% (n=17) had No-NEC. There were significant differences in baseline heart rate (p=0.008), SpO(2) (p<0.001) and A-NIRS (p<0.001) among the three groups. A-NIRS values of NEC piglets remained lower throughout the study with mean for f-NEC of 69 +/- 3.8%, 71.9 +/- 4.04% for nf-NEC, and 78.4 +/- 1.8% for No-NEC piglets (p<0.001). A-NIRS <75% predicted NEC with 97% sensitivity and 97% specificity. NEC piglets demonstrated greater variability from baseline in A-NIRS than healthy piglets (10.1% vs. 6.3%; p=0.04). Mean pI-FABP levels were higher in animals that developed NEC compared to No-NEC piglets (0.66 vs. 0.09 ng/mL; p<0.001). In f-NEC piglets, pI-FABP increased precipitously after feeds (0.04 to 1.87 ng/mL; p<0.001). pI-FABP levels increased in parallel with disease progression and a value > 0.25ng/mL identified animals with NEC (68% sensitivity and 90% specificity). NIRS is a real-time, non-invasive tool that can serve as a diagnostic modality for NEC. In premature piglets, low A-NIRS in the early neonatal period and increased variability during initial feeds are highly predictive of NEC, which is then confirmed by rising plasma I-FABP levels. These modalities may help identify neonates with NEC prior to clinical manifestations of disease. C1 [Zamora, Irving J.; Ethun, Cecilia G.; Sheikh, Fariha; Yu, Ling; Brandt, Mary L.; Olutoye, Oluyinka O.] Texas Childrens Hosp, Baylor Coll Med, Michael E DeBakey Dept Surg, Div Pediat Surg, Houston, TX 77030 USA. [Stoll, Barbara; Burrin, Douglas G.] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. RP Olutoye, OO (reprint author), Texas Childrens Hosp, Baylor Coll Med, Michael E DeBakey Dept Surg, Div Pediat Surg, Houston, TX 77030 USA. EM oolutoye@bcm.edu FU U.S Department of Agriculture [58-6250-6-001]; Texas Medical Center Digestive Diseases Center (National Institutes of Health Grant) [P30 DK-56338]; Texas Children's Hospital Auxiliary fund FX The study was supported, in part, by the U.S Department of Agriculture, under Cooperative Agreement 58-6250-6-001, a pilot grant from the Texas Medical Center Digestive Diseases Center (National Institutes of Health Grant P30 DK-56338), and a grant from the Texas Children's Hospital Auxiliary fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 26 TC 4 Z9 5 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0125437 DI 10.1371/journal.pone.0125437 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500011 PM 26061399 ER PT J AU Zhang, TT Coates, BS Ge, X Bai, SX He, KL Wang, ZY AF Zhang, Tiantao Coates, Brad S. Ge, Xing Bai, Shuxiong He, Kanglai Wang, Zhenying TI Male- and Female-Biased Gene Expression of Olfactory-Related Genes in the Antennae of Asian Corn Borer, Ostrinia furnacalis (Guenee) (Lepidoptera: Crambidae) SO PLOS ONE LA English DT Article ID ODORANT-BINDING-PROTEIN; HOST-PLANT EXPERIENCE; RNA-SEQ DATA; PHEROMONE-BINDING; BOMBYX-MORI; DROSOPHILA-MELANOGASTER; LIGAND-BINDING; MANDUCA-SEXTA; NATURAL VARIATION; INSECT OLFACTION AB The Asian corn borer (ACB), Ostrinia furnacalis (Guenee), is a destructive pest insect of cultivated corn crops, for which antennal-expressed receptors are important to detect olfactory cues for mate attraction and oviposition. Few olfactory related genes were reported in ACB, so we sequenced and characterized the transcriptome of male and female O. furnacalis antennae. Non-normalized male and female O. furnacalis antennal cDNA libraries were sequenced on the Illumina HiSeq 2000 and assembled into a reference transcriptome. Functional gene annotations identified putative olfactory-related genes; 56 odorant receptors (ORs), 23 odorant binding proteins (OBPs), and 10 CSPs. RNA-seq estimates of gene expression respectively showed up-and down-regulation of 79 and 30 genes in female compared to male antennae, which included up-regulation of 8 ORs and 1 PBP gene in male antennae as well as 3 ORs in female antennae. Quantitative real-time RT-PCR analyses validated strong male antennal-biased expression of OfurOR3, 4, 6, 7, 8, 11, 12, 13 and 14 transcripts, whereas OfurOR17 and 18 were specially expressed in female antennae. Sex-biases gene expression described here provides important insight in gene functionalization, and provides candidate genes putatively involved in environmental perception, host plant attraction, and mate recognition. C1 [Zhang, Tiantao; Ge, Xing; Bai, Shuxiong; He, Kanglai; Wang, Zhenying] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Coates, Brad S.] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Wang, ZY (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM zywang@ippcaas.cn FU National Natural Science Foundation of China [31201753]; Special Fund for Agro-scientific Research in the Public Interest [201303026]; United States Department of Agriculture; 565 Agricultural 566 Research Service (USDA-ARS; CRIS Project) [3625-22000-017-00]; Iowa Agriculture 567 and Home Economics Experiment Station, Ames, IA [3543] FX This work was supported by the National Natural Science Foundation of China (31201753) and Special Fund for Agro-scientific Research in the Public Interest (201303026). A portion of research data analyses and interpretation was supported by the United States Department of Agriculture, 565 Agricultural 566 Research Service (USDA-ARS; CRIS Project 3625-22000-017-00) and the Iowa Agriculture 567 and Home Economics Experiment Station, Ames, IA (Project 3543). NR 82 TC 6 Z9 7 U1 11 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0128550 DI 10.1371/journal.pone.0128550 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500088 PM 26062030 ER PT J AU Zhang, YT Liu, J Zhang, JZ Liu, HB Liu, S Zhai, LM Wang, HY Lei, QL Ren, TZ Yin, CB AF Zhang, Yitao Liu, Jian Zhang, Jizong Liu, Hongbin Liu, Shen Zhai, Limei Wang, Hongyuan Lei, Qiuliang Ren, Tianzhi Yin, Changbin TI Row Ratios of Intercropping Maize and Soybean Can Affect Agronomic Efficiency of the System and Subsequent Wheat SO PLOS ONE LA English DT Article ID NORTH CHINA PLAIN; CROPPING SYSTEMS; INTERSPECIFIC INTERACTIONS; NITROGEN-FIXATION; YIELD; GROWTH; SOIL; AGRICULTURE; SOLE; PRODUCTIVITY AB Intercropping is regarded as an important agricultural practice to improve crop production and environmental quality in the regions with intensive agricultural production, e.g., northern China. To optimize agronomic advantage of maize (Zea mays L.) and soybean (Glycine max L.) intercropping system compared to monoculture of maize, two sequential experiments were conducted. Experiment 1 was to screening the optimal cropping system in summer that had the highest yields and economic benefits, and Experiment 2 was to identify the optimum row ratio of the intercrops selected from Experiment 1. Results of Experiment 1 showed that maize intercropping with soybean (maize parallel to soybean) was the optimal cropping system in summer. Compared to conventional monoculture of maize, maize parallel to soybean had significant advantage in yield, economy, land utilization ratio and reducing soil nitrate nitrogen (N) accumulation, as well as better residual effect on the subsequent wheat (Triticum aestivum L.) crop. Experiment 2 showed that intercropping systems reduced use of N fertilizer per unit land area and increased relative biomass of intercropped maize, due to promoted photosynthetic efficiency of border rows and N utilization during symbiotic period. Intercropping advantage began to emerge at tasseling stage after N topdressing for maize. Among all treatments with different row ratios, alternating four maize rows with six soybean rows (4M:6S) had the largest land equivalent ratio (1.30), total N accumulation in crops (258 kg ha(-1)), and economic benefit (3,408 USD ha(-1)). Compared to maize monoculture, 4M:6S had significantly lower nitrate-N accumulation in soil both after harvest of maize and after harvest of the subsequent wheat, but it did not decrease yield of wheat. The most important advantage of 4M:6S was to increase biomass of intercropped maize and soybean, which further led to the increase of total N accumulation by crops as well as economic benefit. In conclusion, alternating four maize rows with six soybean rows was the optimum row ratio in maize parallel to soybean system, though this needs to be further confirmed by pluri-annual trials. C1 [Zhang, Yitao; Zhang, Jizong; Liu, Hongbin; Liu, Shen; Zhai, Limei; Wang, Hongyuan; Lei, Qiuliang; Yin, Changbin] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Nonpoint Source Pollut Control, Minist Agr, Beijing 100081, Peoples R China. [Liu, Jian] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Ren, Tianzhi] Agroenvironm Protect Inst, Minist Agr, Tianjin 300191, Peoples R China. RP Zhang, JZ (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Nonpoint Source Pollut Control, Minist Agr, Beijing 100081, Peoples R China. EM zhangjizong@caas.cn; rentianzhi@caas.cn RI Liu, Jian/P-4050-2014 OI Liu, Jian/0000-0003-4199-1296 FU Special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture, China [201003014, 200903011] FX This study was funded by the Special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture, China (201003014, 200903011). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 44 TC 1 Z9 5 U1 9 U2 40 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2015 VL 10 IS 6 AR e0129245 DI 10.1371/journal.pone.0129245 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK1PO UT WOS:000355979500133 PM 26061566 ER PT J AU Wei, HL Chakravarthy, S Mathieu, J Helmann, TC Stodghill, P Swingle, B Martin, GB Collmer, A AF Wei, Hai-Lei Chakravarthy, Suma Mathieu, Johannes Helmann, Tyler C. Stodghill, Paul Swingle, Bryan Martin, Gregory B. Collmer, Alan TI Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Polymutants Reveal an Interplay between HopAD1 and AvrPtoB SO CELL HOST & MICROBE LA English DT Article ID PLANT NICOTIANA-BENTHAMIANA; CELL-DEATH; TRANSCRIPTION FACTORS; PATHOGEN EFFECTORS; DISEASE RESISTANCE; SYSTEM EFFECTORS; INNATE IMMUNITY; KINASE; GENES; MODEL AB The bacterial pathogen Pseudomonas syringae pv. tomato DC3000 suppresses the two-tiered plant innate immune system by injecting a complex repertoire of type III secretion effector (T3E) proteins. Beyond redundancy and interplay, individual T3Es may interact with multiple immunity-associated proteins, rendering their analysis challenging. We constructed a Pst DC3000 polymutant lacking all 36 T3Es and restored individual T3Es or their mutants to explore the interplay among T3Es. The weakly expressed T3E HopAD1 was sufficient to elicit immunity- associated cell death in Nicotiana benthamiana. HopAD1-induced cell death was suppressed partially by native AvrPtoB and completely by AvrPtoB(M3), which has mutations disrupting its E3 ubiquitin ligase domain and two known domains for interacting with immunity-associated kinases. AvrPtoBM3 also gained the ability to interact with the immunity-kinase MKK2, which is required for HopAD1-dependent cell death. Thus, AvrPtoB has alternative, competing mechanisms for suppressing effector-triggered plant immunity. This approach allows the deconvolution of individual T3E activities. C1 [Wei, Hai-Lei; Chakravarthy, Suma; Helmann, Tyler C.; Swingle, Bryan; Martin, Gregory B.; Collmer, Alan] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Mathieu, Johannes; Martin, Gregory B.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Stodghill, Paul; Swingle, Bryan] ARS, USDA, Ithaca, NY 14853 USA. RP Martin, GB (reprint author), Cornell Univ, Sch Integrat Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM gbm7@cornell.edu; arc2@cornell.edu RI Martin, Gregory/F-6262-2011 OI Martin, Gregory/0000-0003-0044-6830 FU National Science Foundation [IOS-1025642]; National Institutes of Health [R01-GM078021] FX We thank Diane Dunham and Paige Reeves for their assistance in the greenhouse and Noe Fernandez for assistance with the SGN VIGS Tool. Robert B. Abramovitch and Chang-Sik Oh performed preliminary experiments on MKK2. This work was supported by National Science Foundation grant IOS-1025642 (A.C. and G.B.M.) and by National Institutes of Health grant R01-GM078021 (G.B.M.). The authors declare no conflict of interest. NR 42 TC 5 Z9 5 U1 4 U2 28 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1931-3128 EI 1934-6069 J9 CELL HOST MICROBE JI Cell Host Microbe PD JUN 10 PY 2015 VL 17 IS 6 BP 752 EP 762 DI 10.1016/j.chom.2015.05.007 PG 11 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA CK3FI UT WOS:000356101600007 PM 26067603 ER PT J AU Yates, SR Ashworth, DJ Zheng, W Zhang, QP Knutesonn, J van Wessenbeeck, IJ AF Yates, Scott R. Ashworth, Daniel J. Zheng, Wei Zhang, Qiaoping Knutesonn, James van Wessenbeeck, Ian J. TI Emissions of 1,3-Dichloropropene and Chloropicrin after Soil Fumigation under Field Conditions SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soil fumigation; volatilization; emissions; Telone C-35; 1,3-dichloropropene; chloropicrin; bare soil; field experiment; shank injection; Industrial Source Complex Short-Term model (ISCST3); CALPUFF dispersion model; aerodynamic gradient method ID AGRICULTURAL FUMIGANTS; AIR CONCENTRATIONS; DIURNAL PATTERN; VOLATILIZATION; PESTICIDES; EXPOSURE; MODEL; FLUX; DEGRADATION; MITIGATION AB Soil fumigation is an important agronomic practice in the production of many high-value vegetable and fruit crops, but the use of chemical fumigants can lead to excessive atmospheric emissions. A large-scale (2.9 ha) field experiment was conducted to obtain volatilization and cumulative emission rates for two commonly used soil fumigants under typical agronomic practices: 1,3-dichloropropene (1,3-D) and chloropicrin. The aerodynamic method and the indirect back-calculation method using ISCST3 and CALPUFF dispersion models were used to estimate flux loss from the treated field. Over the course of the experiment, the daily peak volatilization rates ranged from 12 to 30 mu g m(-2) s(-1) for 1,3-D and from 0.7 to 2.6 mu g m(-2) s(-1) for chloropicrin. Depending on the method used for quantification, total emissions of 1,3-D and chloropicrin, respectively, ranged from 16 to 35% and from 0.3 to 1.3% of the applied fumigant. A soil incubation study showed that the low volatilization rates measured for chloropicrin were due to particularly high soil degradation rates observed at this field site. Understanding and quantifying fumigant emissions from agricultural soil will help in developing best management practices to reduce emission losses, reducing adverse impacts to human and ecosystem health, and providing inputs for conducting risk assessments. C1 [Yates, Scott R.; Ashworth, Daniel J.; Zhang, Qiaoping] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Ashworth, Daniel J.; Zheng, Wei] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Knutesonn, James; van Wessenbeeck, Ian J.] Dow Agrosci, Indianapolis, IN 46268 USA. RP Yates, SR (reprint author), USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. EM scott.yates@ars.usda.gov FU California Air Resources Board [05-351] FX Part of the research described was supported by the California Air Resources Board (Agreement 05-351). NR 50 TC 5 Z9 5 U1 0 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 10 PY 2015 VL 63 IS 22 BP 5354 EP 5363 DI 10.1021/acs.jafc.5b01309 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CK6CT UT WOS:000356316800006 PM 26001417 ER PT J AU Collison, A Yang, LY Dykes, L Murray, S Awika, JM AF Collison, Amy Yang, Liyi Dykes, Linda Murray, Seth Awika, Joseph M. TI Influence of Genetic Background on Anthocyanin and Copigment Composition and Behavior during Thermoalkaline Processing of Maize SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE anthocyanins; maize; nixtamalization; color stability; food quality ID ANTIOXIDANT ACTIVITY; PHENOLIC-ACIDS; CORN BRAN; PURPLE; COLOR; DIFERULOYLPUTRESCINE; BLUE; RED; PH; FLAVONOIDS AB Visual color is a primary quality factor for foods purchase; identifying factors that influence in situ color quality of pigmented maize during processing is important. Twenty-four genetically distinct pigmented maize hybrids (red/blue, blue, red, and purple) were used to investigate the effect of pigment and copigment composition on color stability during nixtamalization and tortilla chip processing. The red/blue and blue samples generally contained higher proportions of acylated anthocyanins (mainly cyanidin-3-(6 ''-malonylglucoside)) than the red and purple color classes. Phenolic amides were the major extractable copigments in all samples (450-764 mu g/g), with red samples containing the most putrescines and blue samples containing the most spermidines. Even though samples with higher proportions of acylated anthocyanins retained more pigments during processing, this did not relate to final product color quality. In general, the red/blue samples retained their color quality the best and thus are good candidates for genetic improvement for direct processing into alkalized products. C1 [Collison, Amy; Yang, Liyi; Dykes, Linda; Murray, Seth; Awika, Joseph M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Awika, Joseph M.] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA. [Collison, Amy] Frito Lay Inc, Plano, TX 75024 USA. [Yang, Liyi] Kellogg Co, Global Breakfast R&D, Battle Creek, MI 49017 USA. [Dykes, Linda] USDA ARS, Red River Valley Agr Res Ctr, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Awika, JM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM jawika@ag.tamu.edu FU Texas AgriLife Research Monocot Improvement Program FX Funding was provided by Texas AgriLife Research Monocot Improvement Program. NR 36 TC 6 Z9 6 U1 3 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 10 PY 2015 VL 63 IS 22 BP 5528 EP 5538 DI 10.1021/acs.jafc.5b00798 PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CK6CT UT WOS:000356316800026 PM 26010030 ER PT J AU Zhou, DC Zhao, SQ Zhang, LX Sun, G Liu, YQ AF Zhou, Decheng Zhao, Shuqing Zhang, Liangxia Sun, Ge Liu, Yongqiang TI The footprint of urban heat island effect in China SO SCIENTIFIC REPORTS LA English DT Article ID LAND-SURFACE TEMPERATURE; 32 MAJOR CITIES; CLIMATE; MODIS; VALIDATION; INTENSITY; EXPANSION; PHENOLOGY; PRODUCTS; PATTERNS AB Urban heat island (UHI) is one major anthropogenic modification to the Earth system that transcends its physical boundary. Using MODIS data from 2003 to 2012, we showed that the UHI effect decayed exponentially toward rural areas for majority of the 32 Chinese cities. We found an obvious urban/rural temperature "cliff", and estimated that the footprint of UHI effect (FP, including urban area) was 2.3 and 3.9 times of urban size for the day and night, respectively, with large spatiotemporal heterogeneities. We further revealed that ignoring the FP may underestimate the UHI intensity in most cases and even alter the direction of UHI estimates for few cities. Our results provide new insights to the characteristics of UHI effect and emphasize the necessity of considering city- and time-specific FP when assessing the urbanization effects on local climate. C1 [Zhou, Decheng; Zhang, Liangxia] Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Nanjing 210044, Jiangsu, Peoples R China. [Zhou, Decheng; Zhang, Liangxia] Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Nanjing 210044, Jiangsu, Peoples R China. [Zhou, Decheng; Zhao, Shuqing] Peking Univ, Minist Educ, Coll Urban & Environm Sci, Beijing 100871, Peoples R China. [Zhou, Decheng; Zhao, Shuqing] Peking Univ, Minist Educ, Key Lab Earth Surface Proc, Beijing 100871, Peoples R China. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, Raleigh, NC 27606 USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, Athens, GA 30602 USA. RP Zhao, SQ (reprint author), Peking Univ, Minist Educ, Coll Urban & Environm Sci, Beijing 100871, Peoples R China. EM sqzhao@urban.pku.edu.cn FU National Natural Science Foundation of China [31321061]; 111 Project [B14001]; Startup Foundation for Introducing Talent of NUIST [2014r051] FX This study was supported by the National Natural Science Foundation of China (#31321061) and the 111 Project (B14001), and the Startup Foundation for Introducing Talent of NUIST (2014r051). NR 35 TC 15 Z9 17 U1 12 U2 66 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 10 PY 2015 VL 5 AR 11160 DI 10.1038/srep11160 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK3AF UT WOS:000356086600001 PM 26060039 ER PT J AU Yang, SP Zhang, HY Beier, RC Sun, FF Cao, XY Shen, JZ Wang, ZH Zhang, SX AF Yang, Shupeng Zhang, Huiyan Beier, Ross C. Sun, Feifei Cao, Xingyuan Shen, Jianzhong Wang, Zhanhui Zhang, Suxia TI Comparative metabolism of Lappaconitine in rat and human liver microsomes and in vivo of rat using ultra high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry SO JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS LA English DT Article DE Lappaconitine; Metabolism; Liver microsomes; In vivo; UHPLC-Q/TOF-MS ID NORDITERPENOID ALKALOIDS; PROFILING ANALYSIS; HUMAN URINE; ACONITUM; IDENTIFICATION; PLASMA; VITRO; ANTINOCICEPTION; HYDROBROMIDE; SEPARATION AB Lappaconitine (LAP), a non-addictive potent analgesic drug, is broadly used to treat cancer and postoperative pain in many countries, and it also has antibiotic activity against Pseudomonas aeruginosa and Salmonella Typhi. Despite its widespread usage and potential for expanded use, its metabolism was poorly investigated. In this work, the metabolic fate of LAP in liver microsomes of the rat and human was compared, and after oral administration, the metabolites in the rat were investigated using ultra high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q/TOF-MS). As a result, a total of 51 metabolites were identified, including 48 metabolites that were reported here for the first time. Based on accurate MS/MS spectra and the known structure of LAP, the metabolites structures and their fragment ions were readily characterized. The biotransformations of LAP in vitro and in vivo were shown to involve hydroxylation, N-deacetylation, O-demethylation, N-deethylation, and hydrolysis. Furthermore, the results indicated a quantitative species difference in the metabolites for LAP between the rat and human. However, 16-DMLAP, DAL and 5'-OH-DAL were the main in vitro and in vivo metabolites. This work provides the LAP metabolite profiles in rat and human, which will help better understand the pharmacological and toxicological activities of LAP. (C) 2015 Elsevier B.V. All rights reserved. C1 [Yang, Shupeng; Zhang, Huiyan; Sun, Feifei; Cao, Xingyuan; Shen, Jianzhong; Wang, Zhanhui; Zhang, Suxia] China Agr Univ, Coll Vet Med, Beijing Lab Food Qual & Safety, Beijing Key Lab Detect Technol Anim Derived Food, Beijing 100193, Peoples R China. [Beier, Ross C.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Zhang, SX (reprint author), China Agr Univ, Coll Vet Med, 2 Yuanmingyuan Xi Rd, Beijing 100193, Peoples R China. EM suxia@cau.edu.cn FU National Basic Research Program of China [2009CB118801] FX This work was supported financially by the National Basic Research Program of China (Grant No. 2009CB118801). The authors thank Wenli Liu, Xiaoli Song, Ying Wang, Yanshen Li and Haixia Wu for their technical support. NR 38 TC 5 Z9 5 U1 7 U2 63 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0731-7085 EI 1873-264X J9 J PHARMACEUT BIOMED JI J. Pharm. Biomed. Anal. PD JUN 10 PY 2015 VL 110 BP 1 EP 11 DI 10.1016/j.jpba.2015.02.048 PG 11 WC Chemistry, Analytical; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA CG9CA UT WOS:000353610800001 PM 25796978 ER PT J AU Liu, JC Martin, DJ Moon, RJ Youngblood, JP AF Liu, Jen-Chieh Martin, Darren J. Moon, Robert J. Youngblood, Jeffrey P. TI Enhanced thermal stability of biomedical thermoplastic polyurethane with the addition of cellulose nanocrystals SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE biomedical applications; cellulose and other wood products; composites; polyurethanes; thermoplastics ID CELL RESPONSE EVALUATION; SOFT SEGMENT LENGTH; ELASTOMERIC POLYURETHANE; NANOCOMPOSITES; MORPHOLOGY; NANOCELLULOSE; COMPOSITES; WHISKERS; SERIES AB Freeze-dried cellulose nanocrystals (CNCs) were dispersed in the thermoplastic polyurethane [Pellethane 2363-55D (P55D)] by a solvent casting method to fabricate CNC-reinforced nanocomposites. This study demonstrated that the addition of small amounts (1-5 wt %) of CNCs to P55D increased the thermal degradation temperature while maintaining a similar stiffness, strength, and elongation of the neat P55D. CNC additions to P55D did not alter the glass-transition temperature, but the onset decomposition temperature was shifted from 286 to 327 degrees C when 1 wt % CNCs was dispersed in the matrix. The higher onset decomposition temperature was attributed to the formation of hydrogen bonds between the hydroxyl groups on the CNC surface and urethane groups in the hard block of P55D. The ultimate tensile strength and strain to failure (epsilon(f)) of the nanocomposites were minimally affected by additions up to 5 wt % CNCs, whereas the elastic modulus was increased by about 70%. The observation that epsilon(f) was unchanged with the addition of up to 5 wt % CNCs suggested that the flow/sliding of the hard blocks and chains were not hindered by the presence of the CNCs during plastic deformation. The ramifications of this study was that CNC additions resulted in wider processing temperatures of P55D for various biomedical devices while maintaining a similar stiffness, strength, and elongation. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41970. C1 [Liu, Jen-Chieh; Moon, Robert J.; Youngblood, Jeffrey P.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Martin, Darren J.] Univ Queensland, Australian Inst Bioengn & Technol, Brisbane, Qld 4072, Australia. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Moon, Robert J.] Georgia Inst Technol, Renewable Bioprod Inst, Atlanta, GA 30332 USA. RP Moon, RJ (reprint author), Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. EM robertmoon@fs.fed.us; jpyoungb@purdue.edu FU Seed Grant for Purdue/University of Queensland Early Career Researcher Exchange Travel Grant FX The authors thank the Seed Grant for Purdue/University of Queensland Early Career Researcher Exchange Travel Grant for funding this project. They are also grateful to the Forest Products Laboratory (U.S. Forest Service) for providing the freeze-dried CNCs. The authors also appreciate Darren Martin's research group at the Australian Institute for Bioengineering and Nanotechnology (University of Queensland, Australia) for its support with research materials and equipment. NR 37 TC 3 Z9 3 U1 3 U2 83 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD JUN 10 PY 2015 VL 132 IS 22 AR 41970 DI 10.1002/app.41970 PG 8 WC Polymer Science SC Polymer Science GA CD6OY UT WOS:000351209900010 ER PT J AU McLaughlin, JE Bin-Umer, MA Widiez, T Finn, D McCormick, S Tumer, NE AF McLaughlin, John E. Bin-Umer, Mohamed Anwar Widiez, Thomas Finn, Daniel McCormick, Susan Tumer, Nilgun E. TI A Lipid Transfer Protein Increases the Glutathione Content and Enhances Arabidopsis Resistance to a Trichothecene Mycotoxin SO PLOS ONE LA English DT Article ID FUSARIUM HEAD BLIGHT; CELL-DEATH; OXIDATIVE STRESS; SACCHAROMYCES-CEREVISIAE; UDP-GLUCOSYLTRANSFERASE; DEFENSE RESPONSES; REDUCED VIRULENCE; GIBBERELLA-ZEAE; GENE-EXPRESSION; DEOXYNIVALENOL AB Fusarium head blight (FHB) or scab is one of the most important plant diseases worldwide, affecting wheat, barley and other small grains. Trichothecene mycotoxins such as deoxynivalenol (DON) accumulate in the grain, presenting a food safety risk and health hazard to humans and animals. Despite considerable breeding efforts, highly resistant wheat or barley cultivars are not available. We screened an activation tagged Arabidopsis thaliana population for resistance to trichothecin (Tcin), a type B trichothecene in the same class as DON. Here we show that one of the resistant lines identified, trichothecene resistant 1 (trr1) contains a T-DNA insertion upstream of two nonspecific lipid transfer protein (nsLTP) genes, AtLTP4.4 and AtLTP4.5. Expression of both nsLTP genes was induced in trr1 over 10-fold relative to wild type. Overexpression of AtLTP4.4 provided greater resistance to Tcin than AtLTP4.5 in Arabidopsis thaliana and in Saccharomyces cerevisiae relative to wild type or vector transformed lines, suggesting a conserved protection mechanism. Tcin treatment increased reactive oxygen species (ROS) production in Arabidopsis and ROS stain was associated with the chloroplast, the cell wall and the apoplast. ROS levels were attenuated in Arabidopsis and in yeast overexpressing AtLTP4.4 relative to the controls. Exogenous addition of glutathione and other antioxidants enhanced resistance of Arabidopsis to Tcin while the addition of buthionine sulfoximine, an inhibitor of glutathione synthesis, increased sensitivity, suggesting that resistance was mediated by glutathione. Total glutathione content was significantly higher in Arabidopsis and in yeast overexpressing AtLTP4.4 relative to the controls, highlighting the importance of AtLTP4.4 in maintaining the redox state. These results demonstrate that trichothecenes cause ROS accumulation and overexpression of AtLTP4.4 protects against trichothecene-induced oxidative stress by increasing the glutathione-based antioxidant defense. C1 [McLaughlin, John E.; Bin-Umer, Mohamed Anwar; Widiez, Thomas; Finn, Daniel; Tumer, Nilgun E.] Rutgers State Univ, Sch Environm & Biol Sci, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA. [McCormick, Susan] Agr Res Serv, Bacterial Foodborne Pathogens & Mycol Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Tumer, NE (reprint author), Rutgers State Univ, Sch Environm & Biol Sci, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA. EM tumer@aesop.rutgers.edu OI WIDIEZ, Thomas/0000-0001-6002-2306 FU United States Department of Agriculture [59-0206-1-121]; United States Wheat and Barley Scab initiative; National Institutes of Health [S10RR025424] FX This is a cooperative project No. 59-0206-1-121 supported by the United States Department of Agriculture in cooperation with the United States Wheat and Barley Scab initiative and by the National Institutes of Health Grant S10RR025424 to Nilgun E. Tumer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 2 Z9 2 U1 8 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 9 PY 2015 VL 10 IS 6 AR e0130204 DI 10.1371/journal.pone.0130204 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK6PC UT WOS:000356349000082 PM 26057253 ER PT J AU Dickey, AM Kumar, V Morgan, JK Jara-Cavieres, A Shatters, RG McKenzie, CL Osborne, LS AF Dickey, Aaron M. Kumar, Vivek Morgan, J. Kent Jara-Cavieres, Antonella Shatters, Robert G., Jr. McKenzie, Cindy L. Osborne, Lance S. TI A novel mitochondrial genome architecture in thrips (Insecta: Thysanoptera): extreme size asymmetry among chromosomes and possible recent control region duplication SO BMC GENOMICS LA English DT Article ID RNA GENES; PHTHIRAPTERA; EVOLUTION; SEQUENCE AB Background: Multipartite mitochondrial genomes are very rare in animals but have been found previously in two insect orders with highly rearranged genomes, the Phthiraptera (parasitic lice), and the Psocoptera (booklice/barklice). Results: We provide the first report of a multipartite mitochondrial genome architecture in a third order with highly rearranged genomes: Thysanoptera (thrips). We sequenced the complete mitochondrial genomes of two divergent members of the Scirtothrips dorsalis cryptic species complex. The East Asia 1 species has the single circular chromosome common to animals while the South Asia 1 species has a genome consisting of two circular chromosomes. The fragmented South Asia 1 genome exhibits extreme chromosome size asymmetry with the majority of genes on the large, 14.28 kb, chromosome and only nad6 and trnC on the 0.92 kb mini-circle chromosome. This genome also features paralogous control regions with high similarity suggesting a very recent origin of the nad6 mini-circle chromosome in the South Asia 1 cryptic species. Conclusions: Thysanoptera, along with the other minor paraenopteran insect orders should be considered models for rapid mitochondrial genome evolution, including fragmentation. Continued use of these models will facilitate a greater understanding of recombination and other mitochondrial genome evolutionary processes across eukaryotes. C1 [Dickey, Aaron M.; Kumar, Vivek; Morgan, J. Kent; Shatters, Robert G., Jr.; McKenzie, Cindy L.] US Hort Res Lab, Subtrop Insects Res Unit, Ft Pierce, FL 34945 USA. [Dickey, Aaron M.; Kumar, Vivek; Osborne, Lance S.] Univ Florida, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. [Jara-Cavieres, Antonella] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL USA. [Shatters, Robert G., Jr.; McKenzie, Cindy L.] ARS, USDA, Ft Pierce, FL USA. RP Dickey, AM (reprint author), ARS, USDA, Clay Ctr, NE 68933 USA. EM adickey7@hotmail.com FU Floriculture and Nursery Research Initiative; USDA FX The authors thank J Prokop, F Grant, and R Stange for technical support. This research was partially funded by the Floriculture and Nursery Research Initiative, http://www.ars.usda.gov/research/projects/projects.htm?ACCN_NO=402626. Additional funding was provided internally by the USDA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 22 TC 4 Z9 5 U1 2 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 9 PY 2015 VL 16 AR 439 DI 10.1186/s12864-015-1672-4 PG 7 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CK4AD UT WOS:000356158200001 PM 26055161 ER PT J AU Galliher-Beckley, A Pappan, LK Madera, R Burakova, Y Waters, A Nickles, M Li, X Nietfeld, J Schlup, JR Zhong, Q McVey, S Dritz, SS Shi, J AF Galliher-Beckley, A. Pappan, L. K. Madera, Rachel Burakova, Y. Waters, A. Nickles, M. Li, X. Nietfeld, J. Schlup, J. R. Zhong, Q. McVey, S. Dritz, S. S. Shi, J. TI Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines SO VACCINE LA English DT Article DE Adjuvant; Oil-in-water emulsion; Swine influenza virus; Mycoplasma; Vaccine ID MOUTH-DISEASE VIRUS; IMMUNE-RESPONSE; REVERSIBLE INFERTILITY; PEPTIDE VACCINE; PIGS; PERFORMANCE; EFFICACY; ANTIBODY; SIZE; SELF AB Vaccines consisting of subunit or inactivated bacteria/virus and potent adjuvants are widely used to control and prevent infectious diseases. Because inactivated and subunit antigens are often less antigenic than live microbes, a growing need exists for the development of new and improved vaccine adjuvants that can elicit rapid and long-lasting immunity. Here we describe the development and characterization of a novel oil-in-water emulsion, OW-14. OW-14 contains low-cost plant-based emulsifiers and was added to antigen at a ratio of 1:3 with simple hand mixing. OW-14 was stable for prolonged periods of time at temperatures ranging from 4 to 40 degrees C and could be sterilized by autoclaving. Our results showed that OW-14 adjuvanted inactivated swine influenza viruses (SW; H3N2 and H1N1) and Mycoplasma hyopneumoniae (M. hyo) vaccines could be safely administered to piglets in two doses, three weeks apart. Injection sites were monitored and no adverse reactions were observed. Vaccinated pigs developed high and prolonged antibody titers to both Sly and M. hyo. Interestingly, antibody titers were either comparable or greater than those produced by commercially available FluSure (SIV) or RespiSure (M. hyo) vaccines. We also found that OW-14 can induce high antibody responses in pigs that were vaccinated with a decreased antigen dose. This study provides direct evidence that we have developed an easy-to-use and low-cost emulsion that can act as a powerful adjuvant in two common types of swine vaccines. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Galliher-Beckley, A.; Pappan, L. K.; Madera, Rachel; Burakova, Y.; Waters, A.; Nickles, M.; Li, X.; Shi, J.] Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA. [Nietfeld, J.; Dritz, S. S.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Burakova, Y.; Schlup, J. R.] Kansas State Univ, Coll Engn, Dept Chem Engn, Manhattan, KS 66506 USA. [Zhong, Q.] Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA. [McVey, S.] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. RP Shi, J (reprint author), Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA. EM jshi@vet.k-state.edu FU National Bio and Agro-Defense Facility Transition Fund [KBA-CBRI 611310]; NIH [R21 AI085416]; Kansas State University Research Foundation FX We thank Dr. Bob Goodband, Dr. Duane Davis, Mark Nelson, Frank Jennings and the rest of the staff at the Kansas State University Swine Teaching and Research Center for their technical help. Thanks to Dan Boyle and the Biology Department at Kansas State University for their technical help and use of their microscope facility. We thank Dr. Brooke Bloomberg and the rest of the Comparative Medicine staff at Kansas State University for their technical help. We thank Dr. John Bates for his critical reading of this manuscript. This research was supported in part by an award from the National Bio and Agro-Defense Facility Transition Fund, KBA-CBRI 611310, NIH R21 AI085416, and a research grant from Kansas State University Research Foundation. OW-14 adjuvant is patent pending. NR 23 TC 4 Z9 4 U1 1 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUN 9 PY 2015 VL 33 IS 25 BP 2903 EP 2908 DI 10.1016/j.vaccine.2015.04.065 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA CK4JN UT WOS:000356190200007 PM 25936722 ER PT J AU O'Brien, CW Tang, W AF O'Brien, Charles W. Tang, William TI Revision of the New World cycad weevils of the subtribe Allocorynina, with description of two new genera and three new subgenera (Coleoptera: Belidae: Oxycoryninae) SO ZOOTAXA LA English DT Article DE Notorhopalotria; Protocorynus; cycad cone; pollinators; new species ID ZAMIACEAE; POLLINATION; PHYLOGENY; MEXICO AB The taxonomy of the weevils inhabiting male cycad cones in the New World is reviewed. All species belong in a single subtribe, Allocorynina, of the family Belidae, subfamily Oxycoryninae and tribe Oxycorynini and are known to develop only in cones of the cycad genera Dioon and Zamia. Most species of Rhopalotria Chevrolat develop in male cones of Zamia ranging from Mexico, Belize, the Caribbean (Cuba, Isle of Youth, Cayman Islands, Jamaica and the Bahamas) to southern Florida, and one species in those of Dioon spinulosum in Mexico. Rhopalotria consists of three previously described species, two previously described genus-group names (treated herein as subgenera) and four new species described herein: subgenus Allocorynus Sharp with R. calonjei n. sp., R. furfuracea n. sp., R. mollis (Sharp) and R. vovidesi n. sp., and the nominate subgenus Rhopalotria with R. dimidiata Chevrolat, R. meerowi n. sp. and R. slossoni (Schaeffer). The species of Parallocorynus Voss develop only in cones of Dioon in Mexico, and the genus consists of one previously described species, the nominate subgenus and three new subgenera and 11 new species described herein: subgenus Dysicorynus n. subg. with P. andrewsi n. sp. and P. sonorensis n. sp., subgenus Eocorynus n. subg. with P. chemnicki n. sp. and P. schiblii n. sp., subgenus Neocorynus n. subg. with P. iglesiasi n. sp. and P. inexpectatus n. sp., and the nominate subgenus Parallocorynus with P. bicolor (Voss), P. gregoryi n. sp., P. jonesi n. sp., P. norstogi n. sp., P. perezfarrerai n. sp. and P. salasae n. sp. Two new genera are described, Protocorynus with one new species in Honduras, P. bontai, and Notorhopalotria with four new species ranging from Costa Rica to Colombia, N. montgomeryensis, N. panamensis, N. platysoma and N. taylori. Keys to genera, subgenera and species are provided. All of these weevils are believed to be involved in pollination of their host cycads. C1 [Tang, William] USDA, APHIS PPQ South Florida, Miami, FL 33266 USA. RP O'Brien, CW (reprint author), 2313 W Calle Balaustre, Green Valley, AZ 85622 USA. EM cobrien6@cox.net; William.Tang@aphis.usda.gov NR 40 TC 0 Z9 0 U1 1 U2 5 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 9 PY 2015 VL 3970 IS 1 BP 1 EP 87 PG 87 WC Zoology SC Zoology GA CK2DQ UT WOS:000356021200001 PM 26249478 ER PT J AU Cooper, WR Garczynski, SF Horton, DR AF Cooper, W. Rodney Garczynski, Stephen F. Horton, David R. TI Relative Abundance of Carsonella ruddii (Gamma Proteobacterium) in Females and Males of Cacopsylla pyricola (Hemiptera: Psyllidae) and Bactericera cockerelli (Hemiptera: Triozidae) SO JOURNAL OF INSECT SCIENCE LA English DT Article DE bacteriome; mycetome; mycetocyte; Psylloidea; jumping plant lice ID REAL-TIME PCR; CANDIDATUS LIBERIBACTER SOLANACEARUM; ENDOSYMBIONTS; ULTRASTRUCTURE; PSYLLOIDEA; INSECTA; BIOLOGY AB Carsonella ruddii (Gamma Proteobacterium) is an obligate bacterial endosymbiont of psyllids that produces essential amino acids that are lacking in the insect's diet. Accurate estimations of Carsonella populations are important to studies of Carsonella-psyllid interactions and to developing ways to target Carsonella for control of psyllid pests including pear psylla, Cacopsylla pyricola (Forster) (Hemiptera: Psyllidae) and potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae). We used two methods, namely fluorescence in situ hybridization and quantitative polymerase chain reaction (qPCR), to estimate relative abundance of Carsonella in bacteriocytes and whole bodies of psyllids, respectively. Using these two methods, we compared Carsonella populations between female and male insects. Estimations using fluorescence in situ hybridization indicated that Carsonella was more abundant in bacteriocytes of female C. pyricola than in those of males, but Carsonella abundance in bacteriocytes did not differ between sexes of B. cockerelli. Analyses by qPCR using whole-body specimens indicated Carsonella was more abundant in females than in males of both psyllids. Neither fluorescence in situ hybridization nor qPCR indicated that Carsonella populations differed in abundance among adults of different ages (0-3 wk after adult eclosion). Using fluorescence in situ hybridization, Carsonella was observed in ovarioles of newly emerged females and formed an aggregation in the posterior end of mature oocytes. Results of our study indicate that female psyllids harbor greater populations of Carsonella than do males and that sex should be controlled for in studies which require estimations of Carsonella populations. C1 [Cooper, W. Rodney; Garczynski, Stephen F.; Horton, David R.] USDA ARS, Agr Res Lab, Wapato, WA 98951 USA. RP Cooper, WR (reprint author), USDA ARS, Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM Rodney.Cooper@ars.usda.gov RI Cooper, William/D-3205-2017 FU Fresh and Processed Pear Research Committees FX Heather Headrick, Tara Culbertson, and Pauline Anderson provided technical assistance. This work was supported by the Fresh and Processed Pear Research Committees. NR 28 TC 0 Z9 0 U1 3 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUN 8 PY 2015 VL 15 AR UNSP 65 DI 10.1093/jisesa/iev050 PG 5 WC Entomology SC Entomology GA CK6ZW UT WOS:000356379800001 ER PT J AU Manamgoda, DS Rossman, AY Castlebury, LA Chukeatirote, E Hyde, KD AF Manamgoda, Dimuthu S. Rossman, Amy Y. Castlebury, Lisa A. Chukeatirote, Ekachai Hyde, Kevin D. TI A taxonomic and phylogenetic re-appraisal of the genus Curvularia (Pleosporaceae): human and plant pathogens SO PHYTOTAXA LA English DT Article DE Dothideomycetes; Keratitis; Morphology; Opportunistic infections; Pleosporales ID SPECIES RECOGNITION; BIPOLARIS; FUNGI; PHEOHYPHOMYCOSIS; COCHLIOBOLUS; PERITONITIS; GENICULATA; INFECTION; PATIENT; WEB AB Curvularia is an important genus whose species are widely distributed phytopathogens as well as opportunistic pathogens on human and animals. The purpose of this study is to re-evaluate the phylogenetic relationships of the species in the genus Curvularia using ITS (nuclear ribosomal internal transcribed spacer), GPDH (glyceraldehyde-3-phosphate dehydrogenase) and TEF (translation elongation factor) gene regions and to provide modern descriptions and illustrations of Curvularia australis, Curvularia buchloes, C. cymbopogonis, C. hawaiiensis, C. neoindica, C. neergaardii, C. nicotiae, C. nodulosa, C. ryleyi, and C. subpapendorfii which lack recent descriptions with details of host and distribution. A multi-gene phylogenetic tree based on ITS, GPDH and TEF gene regions is used to define species of a fresh collections obtained from various hosts and geographic locations in the world. Both human and plant associated species of Curvularia are included in the phylogenetic analysis. Some species that have previously been described from humans are herein reported from plant material as pathogens or saprobes and vice versa. Novel host associations are reported for C. asianensis, C. borreriae, C. hominis, C. muehlenbeckiae, C. trifolii and C. verruculosa. C1 [Manamgoda, Dimuthu S.; Hyde, Kevin D.] Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China. [Manamgoda, Dimuthu S.; Hyde, Kevin D.] World Agroforestry Ctr, East & Cent Asia, Kunming 650201, Peoples R China. [Manamgoda, Dimuthu S.; Rossman, Amy Y.; Castlebury, Lisa A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Chukeatirote, Ekachai; Hyde, Kevin D.] Mae Fah Luang Univ, Sch Sci, Inst Excellence Fungal Res, Chiang Rai 57100, Thailand. RP Manamgoda, DS (reprint author), Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Biodivers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China. FU U.S. Forest Service (USDA) FX D.S.M. is grateful for the assistance of the U.S. Forest Service (USDA) in sponsoring a visiting studentship at SMML. We thank Mary Barkworth, Megan Romberg (USA), Luis Mejia (Panama), N. Nishihara, and T. Tsukiboshi, (Japan) for the fresh collections and isolates they provided. Hugo Madrid Lorca, Roger Shivas and Yu Pei Tan are thanked for sharing some sequences. The assistance of Shannon Dominick in accessing specimens at BPI and obtaining specimen loans from other herbaria is greatly appreciated. The curators and managers of BRIP and K are thanked for the loan of specimens. Dhanushka Udayanga is thanked for the helpful comments to improve the manuscript. NR 56 TC 6 Z9 6 U1 1 U2 19 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD JUN 8 PY 2015 VL 212 IS 3 BP 175 EP 198 DI 10.11646/phytotaxa.212.3.1 PG 24 WC Plant Sciences SC Plant Sciences GA CK9ZP UT WOS:000356598400001 ER PT J AU Banks, MLL Kremer, RJ Eivazi, F Motavalli, PP Nelson, KA AF Banks, Mona-Lisa L. Kremer, Robert J. Eivazi, Freida Motavalli, Peter P. Nelson, Kelly A. TI Effects of Selected Surfactants on Nutrient Uptake in Corn (Zea mays L.) SO JOURNAL OF PLANT NUTRITION LA English DT Article DE Zea mays; herbicides; surfactants; nutrient uptake ID HUMIC-ACID; CATIONIC SURFACTANTS; SOIL; ADSORPTION; GLYPHOSATE; SORPTION; TILLAGE; TRIFLURALIN; SUBSTANCES; SEDIMENTS AB Surfactants in herbicide formulations eventually enter soil and may disrupt various processes. Research examined effects on nutrient uptake in corn caused by surfactants, herbicides, and surfactant-herbicide combinations applied to silt loam and silty clay loam soils in the greenhouse. Surfactants evaluated were Activator 90, Agri-Dex, and Thrust; herbicides were glyphosate, atrazine, and bentazon. Corn was planted in fertilized soils with moisture content maintained for optimum growth. Foliage (V8 growth stage) was collected for elemental analyses. Nutrient uptake differed with soil texture. Nutrient uptake from silty clay loam was more affected by surfactants and/or herbicides than in silt loam. Potassium uptake was significantly (P = 0.05) decreased in silt loam only by Thrust but uptake of phosphorus (P), potassium (K) calcium (Ca), sulfur (S), copper (Cu), and zinc (Zn) decreased by <= 30% in silty clay loam treated with surfactants. Surfactants and/or herbicides may interact with soil texture to affect nutrient uptake. Long-term field studies to validate changes in nutrient uptake and grain yields after annual applications of surfactants plus herbicides are needed. C1 [Banks, Mona-Lisa L.; Kremer, Robert J.; Motavalli, Peter P.] Univ Missouri, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. [Banks, Mona-Lisa L.; Eivazi, Freida] Lincoln Univ Missouri, Cooperat Res, Jefferson City, MO USA. [Kremer, Robert J.] ARS, USDA, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. [Nelson, Kelly A.] Univ Missouri, Div Plant Sci, Novelty, MO USA. RP Kremer, RJ (reprint author), Univ Missouri, Sch Nat Resources, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. EM kre-merR@missouri.edu NR 47 TC 0 Z9 0 U1 3 U2 22 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0190-4167 EI 1532-4087 J9 J PLANT NUTR JI J. Plant Nutr. PD JUN 7 PY 2015 VL 38 IS 7 BP 1036 EP 1049 DI 10.1080/01904167.2014.881878 PG 14 WC Plant Sciences SC Plant Sciences GA CJ1LE UT WOS:000355244300005 ER PT J AU Nicholson, SJ Nickerson, ML Dean, M Song, Y Hoyt, PR Rhee, H Kim, C Puterka, GJ AF Nicholson, Scott J. Nickerson, Michael L. Dean, Michael Song, Yan Hoyt, Peter R. Rhee, Hwanseok Kim, Changhoon Puterka, Gary J. TI The genome of Diuraphis noxia, a global aphid pest of small grains SO BMC GENOMICS LA English DT Article DE Diuraphis noxia; Russian wheat aphid; Plant-insect interactions; Phytotoxic; Aphid; Genome ID RUSSIAN WHEAT; PEA APHID; ACYRTHOSIPHON-PISUM; HEMIPTERA APHIDIDAE; DNA METHYLATION; MYZUS-PERSICAE; UNITED-STATES; BIOTYPIC DIVERSITY; PLANT-RESPONSES; GENE-EXPRESSION AB Background: The Russian wheat aphid, Diuraphis noxia Kurdjumov, is one of the most important pests of small grains throughout the temperate regions of the world. This phytotoxic aphid causes severe systemic damage symptoms in wheat, barley, and other small grains as a direct result of the salivary proteins it injects into the plant while feeding. Results: We sequenced and de novo assembled the genome of D. noxia Biotype 2, the strain most virulent to resistance genes in wheat. The assembled genomic scaffolds span 393 MB, equivalent to 93% of its 421 MB genome, and contains 19,097 genes. D. noxia has the most AT-rich insect genome sequenced to date (70.9%), with a bimodal CpG(O/E) distribution and a complete set of methylation related genes. The D. noxia genome displays a widespread, extensive reduction in the number of genes per ortholog group, including defensive, detoxification, chemosensory, and sugar transporter groups in comparison to the Acyrthosiphon pisum genome, including a 65% reduction in chemoreceptor genes. Thirty of 34 known D. noxia salivary genes were found in this assembly. These genes exhibited less homology with those salivary genes commonly expressed in insect saliva, such as glucose dehydrogenase and trehalase, yet greater conservation among genes that are expressed in D. noxia saliva but not detected in the saliva of other insects. Genes involved in insecticide activity and endosymbiont-derived genes were also found, as well as genes involved in virus transmission, although D. noxia is not a viral vector. Conclusions: This genome is the second sequenced aphid genome, and the first of a phytotoxic insect. D. noxia's reduced gene content of may reflect the influence of phytotoxic feeding in shaping the D. noxia genome, and in turn in broadening its host range. The presence of methylation-related genes, including cytosine methylation, is consistent with other parthenogenetic and polyphenic insects. The D. noxia genome will provide an important contrast to the A. pisum genome and advance functional and comparative genomics of insects and other organisms. C1 [Nicholson, Scott J.; Puterka, Gary J.] ARS, USDA, Stillwater, OK 74075 USA. [Nickerson, Michael L.; Dean, Michael] NCI, NIH, Bethesda, MD 20892 USA. [Nicholson, Scott J.; Song, Yan; Hoyt, Peter R.] Oklahoma State Univ, Dept Mol Biol & Biochem, Stillwater, OK 74078 USA. [Rhee, Hwanseok; Kim, Changhoon] Axeq Technol, Rockville, MD 20850 USA. RP Puterka, GJ (reprint author), ARS, USDA, Stillwater, OK 74075 USA. EM gary.puterka@ars.usda.gov NR 104 TC 5 Z9 5 U1 8 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 5 PY 2015 VL 16 AR 429 DI 10.1186/s12864-015-1525-1 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DA1PV UT WOS:000367568700001 PM 26044338 ER PT J AU Karki, TB Sah, SK Thapa, RB McDonald, AJ Davis, AS AF Karki, Tika B. Sah, Shrawan K. Thapa, Resam B. McDonald, Andrew J. Davis, Adam S. TI Identifying Pathways for Improving Household Food Self-Sufficiency Outcomes in the Hills of Nepal SO PLOS ONE LA English DT Article ID SECURITY; MANAGEMENT; MIDHILLS; MAIZE; YIELD AB Maintaining and improving household food self-sufficiency (FSS) in mountain regions is an ongoing challenge. There are many facets to the issue, including comparatively high levels of land fragmentation, challenging terrain and transportation bottlenecks, declining labor availability due to out-migration, and low technical knowledge, among others. Using a nonparametric multivariate approach, we quantified primary associations underlying current levels of FSS in the mid-hills of Nepal. A needs assessment survey was administered to 77 households in Lungaun (Baglung District), Pang (Parbat District), and Pathlekhet (Myagdi District), with a total of 80 variables covering five performance areas; resulting data were analyzed using Classification and Regression Trees. The most parsimonious statistical model for household FSS highlighted associations with agronomic management, including yields of maize and fingermillet within a relay cropping system and adoption of improved crop cultivars. Secondary analyses of the variables retained in the first model again focused primarily on crop and livestock management. It thus appears that continued emphasis on technical agricultural improvements is warranted, independent of factors such as land holding size that, in any case, are very difficult to change through development interventions. Initiatives to increase household FSS in the mid-hills of Nepal will benefit from placing a primary focus on methods of agricultural intensification to improve crop yields and effective technology transfer to increase adoption of these methods. C1 [Karki, Tika B.; Sah, Shrawan K.; Thapa, Resam B.] Tribhuvan Univ, Inst Agr & Anim Sci, Rampur, Nepal. [McDonald, Andrew J.] CIMMYT, South Asia Reg Off, Kathmandu, Nepal. [Davis, Adam S.] USDA ARS, Urbana, IL 61801 USA. RP Davis, AS (reprint author), USDA ARS, Urbana, IL 61801 USA. EM adam.davis@ars.usda.gov FU Bill and Melinda Gates Foundation; US Agency for International Development through the International Maize and Wheat Improvement Center (CIMMYT), Mexico FX T. B. Karki received fellowship support of his doctoral research from the Bill and Melinda Gates Foundation and US Agency for International Development through the International Maize and Wheat Improvement Center (CIMMYT), Mexico. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 25 TC 0 Z9 0 U1 2 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 5 PY 2015 VL 10 IS 6 AR e0127513 DI 10.1371/journal.pone.0127513 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ7CR UT WOS:000355652200022 PM 26047508 ER PT J AU Perera, OP Shelby, KS Popham, HJR Gould, F Adang, MJ Jurat-Fuentes, JL AF Perera, Omaththage P. Shelby, Kent S. Popham, Holly J. R. Gould, Fred Adang, Michael J. Jurat-Fuentes, Juan Luis TI Generation of a Transcriptome in a Model Lepidopteran Pest, Heliothis virescens, Using Multiple Sequencing Strategies for Profiling Midgut Gene Expression SO PLOS ONE LA English DT Article ID BACILLUS-THURINGIENSIS CRY1A; ALKALINE-PHOSPHATASE CDNA; DROSOPHILA S2 CELLS; RNA INTERFERENCE; BINDING-PROTEINS; AMINOPEPTIDASE-N; SEX-PHEROMONE; BOMBYX-MORI; COTTON BOLLWORM; DESERT LOCUST AB Heliothine pests such as the tobacco budworm, Heliothis virescens (F.), pose a significant threat to production of a variety of crops and ornamental plants and are models for developmental and physiological studies. The efforts to develop new control measures for H. virescens, as well as its use as a relevant biological model, are hampered by a lack of molecular resources. The present work demonstrates the utility of next-generation sequencing technologies for rapid molecular resource generation from this species for which lacks a sequenced genome. In order to amass a de novo transcriptome for this moth, transcript sequences generated from Illumina, Roche 454, and Sanger sequencing platforms were merged into a single de novo transcriptome assembly. This pooling strategy allowed a thorough sampling of transcripts produced under diverse environmental conditions, developmental stages, tissues, and infections with entomopathogens used for biological control, to provide the most complete transcriptome to date for this species. Over 138 million reads from the three platforms were assembled into the final set of 63,648 contigs. Of these, 29,978 had significant BLAST scores indicating orthologous relationships to transcripts of other insect species, with the top-hit species being the monarch butterfly (Danaus plexippus) and silkworm (Bombyx mori). Among identified H. virescens orthologs were immune effectors, signal transduction pathways, olfactory receptors, hormone biosynthetic pathways, peptide hormones and their receptors, digestive enzymes, and insecticide resistancgenomics and for new biological experimentation needed to develop efficient control efforts for this and related Noctuid pest moths.e enzymes. As an example, we demonstrate the utility of this transcriptomic resource to study gene expression profiling of larval midguts and detect transcripts of putative Bacillus thuringiensis (Bt) Cry toxin receptors. The substantial molecular resources described in this study will facilitate development of H. virescens as a relevant biological model for functional C1 [Perera, Omaththage P.] ARS, Southern Insect Management Res Unit, USDA, Stoneville, MS 38776 USA. [Shelby, Kent S.; Popham, Holly J. R.] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Gould, Fred] N Carolina State Univ, Dept Entomol, Raleigh, NC 27607 USA. [Adang, Michael J.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Jurat-Fuentes, Juan Luis] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. RP Perera, OP (reprint author), ARS, Southern Insect Management Res Unit, USDA, Stoneville, MS 38776 USA. EM OP.Perera@ars.usda.gov OI Jurat-Fuentes, Juan Luis/0000-0002-8945-1814 FU U. S. Department of Agriculture under National Research Initiative Award [2004-35607-19935]; U. S. Department of Agriculture under Biotechnology Risk Assessment Grant [2008-03046] FX This work was partially supported by the U. S. Department of Agriculture under National Research Initiative Award No. 2004-35607-19935 to M.J.A. and J.L.J.-F., and Biotechnology Risk Assessment Grant Award No. 2008-03046 to J.L.J.-F. and O.P.P. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 105 TC 2 Z9 3 U1 2 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 5 PY 2015 VL 10 IS 6 AR e0128563 DI 10.1371/journal.pone.0128563 PG 28 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ7CR UT WOS:000355652200079 PM 26047101 ER PT J AU Runyon, JB AF Runyon, Justin B. TI Haromyia, a new genus of long-legged flies from Dominica (Diptera: Dolichopodidae) SO ZOOTAXA LA English DT Article DE Neotropical; micro-dolichopodid; Enlinia; Harmstonia; West Indies; Achalcinae; clypeal setae ID SYSTEMATIC REVISION; PHYLOGENY; ZOOGEOGRAPHY; ACHALCINAE; ECOLOGY AB The new micro-dolichopodid genus Haromyia gen. nov. and the type species H. iviei sp. nov. are described from the island of Dominica in the Lesser Antilles. Males and females of Haromyia are distinguished by the large setae on a bulging clypeus, minute size, and wing veins that are nearly straight and evenly diverging from wing base. Haromyia does not fit readily into any contemporary dolichopodid subfamily, although it superficially resembles the Enliniinae and Achalcinae. Haromyia should be regarded as incertae sedis until the dolichopodid subfamilies can be refined, particularly to better incorporate the tropical diversity of this large family. C1 [Runyon, Justin B.] US Forest Serv, Rocky Mt Res Stn, USDA, Bozeman, MT 59717 USA. [Runyon, Justin B.] Montana State Univ, Montana Entomol Collect, Bozeman, MT 59717 USA. RP Runyon, JB (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, 1648 S 7th Ave, Bozeman, MT 59717 USA. EM jrunyon@montana.edu FU PECASE (President's Early Career Award in Science and Engineering) FX Thanks to Dr. Michael Ivie (Montana State University) for providing specimens and for allowing access to a Syncroscopy AutoMontagePro (R) system to create digital images. Thanks to Harold Robinson (USNM) for looking at photographs of this fly and providing comments. Dan Bickel, Scott Brooks and Renato Capellari provided helpful comments on the manuscript. This work was supported by funding from PECASE (President's Early Career Award in Science and Engineering) to Justin Runyon. NR 20 TC 0 Z9 0 U1 1 U2 5 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 5 PY 2015 VL 3964 IS 5 BP 589 EP 595 PG 7 WC Zoology SC Zoology GA CJ7OV UT WOS:000355687200010 PM 26249471 ER PT J AU Kapheim, KM Pan, HL Li, C Salzberg, SL Puiu, D Magoc, T Robertson, HM Hudson, ME Venkat, A Fischman, BJ Hernandez, A Yandell, M Ence, D Holt, C Yocum, GD Kemp, WP Bosch, J Waterhouse, RM Zdobnov, EM Stolle, E Kraus, FB Helbing, S Moritz, RFA Glastad, KM Hunt, BG Goodisman, MAD Hauser, F Grimmelikhuijzen, CJP Pinheiro, DG Nunes, FMF Soares, MPM Tanaka, ED Simoes, ZLP Hartfelder, K Evans, JD Barribeau, SM Johnson, RM Massey, JH Southey, BR Hasselmann, M Hamacher, D Biewer, M Kent, CF Zayed, A Blatti, C Sinha, S Johnston, JS Hanrahan, SJ Kocher, SD Wang, J Robinson, GE Zhang, GJ AF Kapheim, Karen M. Pan, Hailin Li, Cai Salzberg, Steven L. Puiu, Daniela Magoc, Tanja Robertson, Hugh M. Hudson, Matthew E. Venkat, Aarti Fischman, Brielle J. Hernandez, Alvaro Yandell, Mark Ence, Daniel Holt, Carson Yocum, George D. Kemp, William P. Bosch, Jordi Waterhouse, Robert M. Zdobnov, Evgeny M. Stolle, Eckart Kraus, F. Bernhard Helbing, Sophie Moritz, Robin F. A. Glastad, Karl M. Hunt, Brendan G. Goodisman, Michael A. D. Hauser, Frank Grimmelikhuijzen, Cornelis J. P. Pinheiro, Daniel Guariz Franco Nunes, Francis Morais Miranda Soares, Michelle Prioli Tanaka, Erica Donato Paulino Simoes, Zila Luz Hartfelder, Klaus Evans, Jay D. Barribeau, Seth M. Johnson, Reed M. Massey, Jonathan H. Southey, Bruce R. Hasselmann, Martin Hamacher, Daniel Biewer, Matthias Kent, Clement F. Zayed, Amro Blatti, Charles, III Sinha, Saurabh Johnston, J. Spencer Hanrahan, Shawn J. Kocher, Sarah D. Wang, Jun Robinson, Gene E. Zhang, Guojie TI Genomic signatures of evolutionary transitions from solitary to group living SO SCIENCE LA English DT Article ID TRANSPOSABLE ELEMENTS; POPULATION GENOMICS; POSITIVE SELECTION; SOCIAL INSECTS; PATTERNS; BEHAVIOR; PROTEIN; GENES; RATES AB The evolution of eusociality is one of the major transitions in evolution, but the underlying genomic changes are unknown. We compared the genomes of 10 bee species that vary in social complexity, representing multiple independent transitions in social evolution, and report three major findings. First, many important genes show evidence of neutral evolution as a consequence of relaxed selection with increasing social complexity. Second, there is no single road map to eusociality; independent evolutionary transitions in sociality have independent genetic underpinnings. Third, though clearly independent in detail, these transitions do have similar general features, including an increase in constrained protein evolution accompanied by increases in the potential for gene regulation and decreases in diversity and abundance of transposable elements. Eusociality may arise through different mechanisms each time, but would likely always involve an increase in the complexity of gene networks. C1 [Kapheim, Karen M.; Robertson, Hugh M.; Hudson, Matthew E.; Venkat, Aarti; Fischman, Brielle J.; Blatti, Charles, III; Sinha, Saurabh; Robinson, Gene E.] Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA. [Kapheim, Karen M.; Robertson, Hugh M.; Massey, Jonathan H.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Kapheim, Karen M.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Pan, Hailin; Li, Cai; Wang, Jun; Zhang, Guojie] BGI Shenzhen, China Natl GeneBank, Shenzhen 518083, Peoples R China. [Li, Cai] Univ Copenhagen, Nat Hist Museum Denmark, Ctr GeoGenet, DK-1350 Copenhagen, Denmark. [Salzberg, Steven L.] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA. [Salzberg, Steven L.] Johns Hopkins Univ, Dept Comp Sci, Baltimore, MD 21218 USA. [Salzberg, Steven L.] Johns Hopkins Univ, Dept Biostat, Baltimore, MD 21218 USA. [Salzberg, Steven L.; Puiu, Daniela; Magoc, Tanja] Johns Hopkins Univ, McKusick Nathans Inst Genet Med, Ctr Computat Biol, Sch Med, Baltimore, MD 21205 USA. [Hudson, Matthew E.; Venkat, Aarti] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Venkat, Aarti] Univ Chicago, Dept Human Genet, Chicago, IL 60637 USA. [Fischman, Brielle J.] Univ Illinois, Program Ecol & Evolutionary Biol, Urbana, IL 61801 USA. [Fischman, Brielle J.] Hobart & William Smith Coll, Dept Biol, Geneva, NY 14456 USA. [Hernandez, Alvaro] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. [Yandell, Mark; Ence, Daniel; Holt, Carson] Univ Utah, Eccles Inst Human Genet, Dept Human Genet, Salt Lake City, UT 84112 USA. [Yandell, Mark; Holt, Carson] Univ Utah, USTAR Ctr Genet Discovery, Salt Lake City, UT 84112 USA. [Yocum, George D.; Kemp, William P.] ARS, USDA, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND 58102 USA. [Bosch, Jordi] Univ Autonoma Barcelona, Ctr Ecol Res & Forestry Applicat CREAF, Bellaterra 08193, Spain. [Waterhouse, Robert M.; Zdobnov, Evgeny M.] Univ Geneva, Sch Med, Dept Genet Med & Dev, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.; Zdobnov, Evgeny M.] Swiss Inst Bioinformat, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA. [Waterhouse, Robert M.] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA. [Stolle, Eckart; Kraus, F. Bernhard; Helbing, Sophie; Moritz, Robin F. A.] Univ Halle Wittenberg, Dept Zool, Inst Biol, D-06099 Halle, Saale, Germany. [Stolle, Eckart] Mary Univ London, Sch Biol & Chem Sci, Organismal Biol Res Grp, London E1 4NS, England. [Kraus, F. Bernhard] Univ Hosp Halle, Dept Lab Med, D-06120 Halle, Saale, Germany. [Moritz, Robin F. A.] German Ctr Integrat Biodivers Res iDiv, D-04103 Leipzig, Germany. [Glastad, Karl M.; Goodisman, Michael A. D.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Hunt, Brendan G.] Univ Georgia, Dept Entomol, Griffin, GA 30223 USA. [Hauser, Frank; Grimmelikhuijzen, Cornelis J. P.] Univ Copenhagen, Dept Biol, Ctr Funct & Comparat Insect Genom, Copenhagen, Denmark. [Pinheiro, Daniel Guariz; Miranda Soares, Michelle Prioli; Paulino Simoes, Zila Luz] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil. [Pinheiro, Daniel Guariz] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Tecnol, BR-14884900 Jaboticabal, SP, Brazil. [Franco Nunes, Francis Morais] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP, Brazil. [Tanaka, Erica Donato] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil. [Hartfelder, Klaus] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049900 Ribeirao Preto, SP, Brazil. [Evans, Jay D.] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Barribeau, Seth M.] E Carolina Univ, Dept Biol, Greenville, NC 27858 USA. [Johnson, Reed M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Massey, Jonathan H.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [Southey, Bruce R.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Hasselmann, Martin; Hamacher, Daniel; Biewer, Matthias] Univ Hohenheim, Inst Anim Husb & Anim Breeding, Dept Populat Genom, Stuttgart, Germany. [Kent, Clement F.; Zayed, Amro] York Univ, Dept Biol, Toronto, ON M3J 1P3, Canada. [Kent, Clement F.] Howard Hughes Med Inst, Ashburn, VA 20147 USA. [Blatti, Charles, III; Sinha, Saurabh] Univ Illinois, Dept Comp Sci, Urbana, IL 61801 USA. [Johnston, J. Spencer; Hanrahan, Shawn J.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Kocher, Sarah D.] Harvard Univ, Museum Comparat Zool, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Wang, Jun] Univ Copenhagen, Dept Biol, DK-2200 Copenhagen, Denmark. [Wang, Jun] King Abdulaziz Univ, Princess Al Jawhara Ctr Excellence Res Hereditary, Jeddah 21589, Saudi Arabia. [Wang, Jun] Macau Univ Sci & Technol, Taipa 999078, Macau, Peoples R China. [Wang, Jun] Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China. [Robinson, Gene E.] Univ Illinois, Ctr Adv Study Prof Entomol & Neurosci, Urbana, IL 61801 USA. [Zhang, Guojie] Univ Copenhagen, Dept Biol, Ctr Social Evolut, DK-2100 Copenhagen, Denmark. RP Kapheim, KM (reprint author), Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA. EM karen.kapheim@usu.edu; wangj@genomics.org.cn; generobi@illinois.edu; zhanggj@genomics.org.cn RI Moritz, Robin/K-6053-2012; Wang, Jun/B-9503-2016; Kraus, Frank Bernhard/B-8172-2011; Hudson, Matthew/A-4438-2008; Hartfelder, Klaus/A-4293-2009; Johnson, Reed/H-3742-2011; Hauser, Frank/M-2952-2014; Evans, Jay/C-8408-2012; iDiv, Deutsches Zentrum/B-5164-2016; Wang, Jun/C-8434-2016; Waterhouse, Robert/A-1858-2010; Kent, Clement/B-9982-2008; Nunes, Francis/F-5871-2010; Hunt, Brendan/B-9816-2009; Zdobnov, Evgeny/K-1133-2012 OI Moritz, Robin/0000-0003-0791-887X; Stolle, Eckart/0000-0001-7638-4061; Wang, Jun/0000-0002-2113-5874; Grimmelikhuijzen, Cornelis/0000-0001-6486-2046; Bosch , Jordi/0000-0002-8088-9457; Kraus, Frank Bernhard/0000-0003-4354-9952; Hudson, Matthew/0000-0002-4737-0936; Hartfelder, Klaus/0000-0001-7981-8427; Hauser, Frank/0000-0001-5563-2345; Evans, Jay/0000-0002-0036-4651; Wang, Jun/0000-0002-8540-8931; Waterhouse, Robert/0000-0003-4199-9052; Kent, Clement/0000-0003-4994-0098; Nunes, Francis/0000-0002-7769-3058; Hunt, Brendan/0000-0002-0030-9302; FU BGI, a U.S. National Institutes of Health Pioneer Award [DP1 OD006416]; European Union [300837]; U.S. National Science Foundation [DEB-0640690, DEB-0743154]; Danish Council for Independent Research [10-081390, 0602-01170B]; Lundbeck Foundation; Georgia Tech-Elizabeth Smithgall Watts endowment; Marie Curie International Outgoing Fellowship [PIOF-GA-2011-303312]; Swiss National Science Foundation [31003A-125350]; Commission Informatique of the University of Geneva; Schmidheiny Foundation; Swiss Institute of Bioinformatics; Natural Sciences and Engineering Research Council of Canada; i5K Initiative FX Data deposition at National Center for Biotechnology Information: H. laboriosa, D. novaeangliae, E. mexicana, M. quadrifasciata, and M. rotundata genome assemblies accession numbers PRJNA279436, PRJNA279825, PRJNA279814, PRJNA279820, and PRJNA66515. Funding for genome sequencing and analysis: BGI, a U.S. National Institutes of Health Pioneer Award (DP1 OD006416) to G.E.R., and European Union Marie Curie International Incoming Fellowship (300837) to G.Z. Additional funding: U.S. National Science Foundation grant DEB-0640690 (M.A.D.G.) and DEB-0743154 (G.E.R. and M.E.H); Danish Council for Independent Research grants 10-081390 (C.L.) and 0602-01170B (C.J.P.G.); Lundbeck Foundation (C.J.P.G); Georgia Tech-Elizabeth Smithgall Watts endowment (M.A.D.G.); Marie Curie International Outgoing Fellowship PIOF-GA-2011-303312 (R.M.W.); Swiss National Science Foundation award 31003A-125350, Commission Informatique of the University of Geneva, Schmidheiny Foundation, and Swiss Institute of Bioinformatics (E.M.Z.); and Natural Sciences and Engineering Research Council of Canada Discovery Grant and Early Research Award (A.Z.). This project was conducted under the auspices of the i5K Initiative. We thank the Roy J. Carver Biotechnology Center (sequencing services); N. Lartillot (advice on Coevol); T. Newman (assistance with DNA extractions); and E. Hadley (assistance with figures). Computational support: D. Davidson, N. Band, D. Slater (University of Illinois), J. H. Kidner and H. Scharpenberg (Martin-Luther-University Halle-Wittenberg); Compute Canada; and Center for High Performance Computing at the University of Utah. J. Himes created the illustrations in Fig. 1. We are grateful to T. Pitts-Singer, H. G. Hall, B. N. Danforth, J. Gibbs, and S. Cardinal for providing bee specimens; R. Ayala for identification of E. mexicana; J. Vega and the staff at Estacion de Biologia Chamela, Universidad Nacional Autonoma de Mexico (UNAM), for support during collecting trips; and S. A. Cameron for insightful discussion. NR 28 TC 41 Z9 42 U1 26 U2 122 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JUN 5 PY 2015 VL 348 IS 6239 BP 1139 EP 1143 DI 10.1126/science.aaa4788 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ6FY UT WOS:000355590500049 PM 25977371 ER PT J AU Xu, WH Meng, Y Surana, P Fuerst, G Nettleton, D Wise, RP AF Xu, Weihui Meng, Yan Surana, Priyanka Fuerst, Greg Nettleton, Dan Wise, Roger P. TI The knottin-like Blufensin family regulates genes involved in nuclear import and the secretory pathway in barley-powdery mildew interactions SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE knottin; nuclear import; secretory pathway; powdery mildew; calmodulin; BSMV-VIGS; gene expression; negative regulator ID BACTERIAL-BLIGHT RESISTANCE; PLANT IMMUNE-RESPONSES; DISEASE-RESISTANCE; PROTEIN TRANSLOCATION; ARABIDOPSIS-THALIANA; CALMODULIN-BINDING; TRIGGERED IMMUNITY; BLUMERIA-GRAMINIS; AVIRULENCE GENES; BAX INHIBITOR-1 AB Plants have evolved complex regulatory mechanisms to control a multi-layered defense response to microbial attack. Both temporal and spatial gene expression are tightly regulated in response to pathogen ingress, modulating both positive and negative control of defense. BLUFENSINs, small knottin-like peptides in barley, wheat, and rice, are highly induced by attack from fungal pathogens, in particular, the obligate biotrophic fungus, Blumeria graminis f. sp. hordei (Bgh), causal agent of barley powdery mildew. Previous research indicated that Blufensin1 (Bln1) functions as a negative regulator of basal defense mechanisms. In the current report, we show that BLN1 and BLN2 can both be secreted to the apoplast and Barley stripe mosaic virus (BSMV)-mediated overexpression of Bln2 increases susceptibility of barley to Bgh. Bimolecular fluorescence complementation (BiFC) assays signify that BLN1 and BLN2 can interact with each other, and with calmodulin. We then used BSMV-induced gene silencing to knock down Bln1, followed by Barley1 GeneChip transcriptome analysis, to identify additional host genes influenced by Bln1. Analysis of differential expression revealed a gene set enriched for those encoding proteins annotated to nuclear import and the secretory pathway, particularly lmportin alpha 1-b and Sec61 gamma subunits. Further functional analysis of these two affected genes showed that when silenced, they also reduced susceptibility to Bgh. Taken together, we postulate that Bln1 is co-opted by Bgh to facilitate transport of disease-related host proteins or effectors, influencing the establishment of Bgh compatibility on its barley host. C1 [Xu, Weihui; Meng, Yan; Surana, Priyanka; Fuerst, Greg; Wise, Roger P.] Iowa State Univ, Ctr Plant Responses Environm Stresses, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Surana, Priyanka] Iowa State Univ, Bioinformat & Computat Biol Grad Program, Ames, IA 50011 USA. [Fuerst, Greg; Wise, Roger P.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. RP Wise, RP (reprint author), Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, 351 Bessey Hall, Ames, IA 50011 USA. EM roger.wise@ars.usda.gov FU National Science Foundation Plant Genome grant [05-00461, 09-22746, 13-39348] FX We thank Matt Moscou for initial analysis of the Barley1 GeneChip dataset. This research was supported by National Science Foundation Plant Genome grants 05-00461, 09-22746, and 13-39348. This article is a joint contribution of the Iowa Agriculture and Home Economics Experiment Station and the Corn Insects and Crop Genetics Research Unit, USDA-Agricultural Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or the National Science Foundation. NR 108 TC 1 Z9 1 U1 1 U2 18 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 4 PY 2015 VL 6 AR 409 DI 10.3389/fpls.2015.00409 PG 18 WC Plant Sciences SC Plant Sciences GA CL6CW UT WOS:000357056200001 PM 26089830 ER PT J AU Saxton-Shaw, KD Ledermann, JP Kenney, JL Berl, E Graham, AC Russo, JM Powers, AM Mutebi, JP AF Saxton-Shaw, Kali D. Ledermann, Jeremy P. Kenney, Joan L. Berl, Erica Graham, Alan C. Russo, Joel M. Powers, Ann M. Mutebi, John-Paul TI The First Outbreak of Eastern Equine Encephalitis in Vermont: Outbreak Description and Phylogenetic Relationships of the Virus Isolate SO PLOS ONE LA English DT Article ID ENCEPHALOMYELITIS VIRUS; ARBOVIRUS ISOLATIONS; MOSQUITOS DIPTERA; CULICIDAE; STRAINS AB The first known outbreak of eastern equine encephalitis (EEE) in Vermont occurred on an emu farm in Rutland County in 2011. The first isolation of EEE virus (EEEV) in Vermont (VT11) was during this outbreak. Phylogenetic analysis revealed that VT11 was most closely related to FL01, a strain from Florida isolated in 2001, which is both geographically and temporally distinct from VT11. EEEV RNA was not detected in any of the 3,905 mosquito specimens tested, and the specific vectors associated with this outbreak are undetermined. C1 [Saxton-Shaw, Kali D.; Ledermann, Jeremy P.; Kenney, Joan L.; Powers, Ann M.; Mutebi, John-Paul] Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Ft Collins, CO 80521 USA. [Berl, Erica] Vermont Dept Hlth, Burlington, VT 05402 USA. [Graham, Alan C.] Vermont Agcy Agr Food & Markets, Waterbury, VT USA. [Russo, Joel M.] USDA, APHIS VS, Montpelier, VT USA. RP Mutebi, JP (reprint author), Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Ft Collins, CO 80521 USA. EM grv0@cdc.gov NR 32 TC 2 Z9 2 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 4 PY 2015 VL 10 IS 6 AR e0128712 DI 10.1371/journal.pone.0128712 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ7TC UT WOS:000355701600062 PM 26043136 ER PT J AU Whaley, A Sheridan, J Safari, S Burton, A Burkey, K Schlueter, J AF Whaley, Adam Sheridan, Jaime Safari, Sajedeh Burton, Amy Burkey, Kent Schlueter, Jessica TI RNA-seq analysis reveals genetic response and tolerance mechanisms to ozone exposure in soybean SO BMC GENOMICS LA English DT Article ID WATER-USE EFFICIENCY; TROPOSPHERIC OZONE; QUALITY-CONTROL; CLIMATE-CHANGE; ASCORBIC-ACID; STRESS; EXPRESSION; PLANTS; INJURY; CROPS AB Background: Oxidative stress caused by ground level ozone is a contributor to yield loss in a number of important crop plants. Soybean (Glycine max) is considered to be ozone sensitive, and current research into its response to oxidative stress is limited. To better understand the genetic response in soybean to oxidative stress, an RNA-seq analysis of two soybean cultivars was performed comparing an ozone intolerant cultivar (Mandarin-Ottawa) and an ozone resistant cultivar (Fiskeby III) following exposure to ozone. Results: Analysis of the transcriptome data revealed cultivar-specific expression level differences of genes previously implicated in oxidative stress responses, indicating unique cultivar-specific responses. Both Fiskeby III and Mandarin (Ottawa) exhibit an increased expression of oxidative response genes as well as glutathiones, phenylpropanoids, and phenylalanine ammonia-lyases. Mandarin (Ottawa) exhibited more general stress response genes whereas Fiskeby III had heightened expression of metabolic process genes. An examination of the timing of gene responses over the course of ozone exposure identified significantly more differentially expressed genes across all time points in Mandarin (Ottawa) than in Fiskeby III. The timing of expression was also considered to identify genes that may be indicative of a delayed response to ozone stress in Fiskeby III, We found that Mandarin (Ottawa) exhibits an higher level of expression in early time points for oxidative and general stress response genes while Fiskeby III seems to maintain expression of defense and stress response genes. Of particular interest was the expression of wax and cutin biosynthetic genes that we found to be expressed in Mandarin (Ottawa) in all sampled time points, whereas the expression of this pathway is only in the first time point for Fiskeby III. Conclusions: We were able to identify differentially expressed genes that correspond to each of the known or expected categories of genes previously implicated in other species for ozone stress. Our study shows evidence that at least part of the observed ozone tolerance of Fiskeby III may be due to its thicker, denser leaves providing passive resistance thereby limiting the degree of ozone exposure. The observed diminished genetic response is then likely a consequence of this reduced exposure. C1 [Whaley, Adam; Sheridan, Jaime; Safari, Sajedeh; Schlueter, Jessica] Univ N Carolina, Dept Bioinformat & Genom, Charlotte, NC 28223 USA. [Burton, Amy; Burkey, Kent] USDA ARS, Raleigh, NC 27695 USA. RP Schlueter, J (reprint author), Univ N Carolina, Dept Bioinformat & Genom, 9201 Univ City Blvd, Charlotte, NC 28223 USA. EM jschluet@uncc.edu FU USDA-ARS CRIS [6645-11000-008-00D]; United Soybean Board [0286]; NSF [1100695, 0822258]; UNC-Charlotte FX The authors thank Renee Tucker for assistance with plant growth and Jeff Barton for operating the ozone exposure system. Funding for Kent Burkey and Amy Burton provided by USDA-ARS CRIS# 6645-11000-008-00D and United Soybean Board grant # 0286. Adam Whaley was supported by NSF Award # 1100695 and Sajedeh Safari was supported by NSF Award # 0822258. Funding for the genomics experiments was provided by UNC-Charlotte. NR 48 TC 1 Z9 1 U1 5 U2 36 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 4 PY 2015 VL 16 AR 426 DI 10.1186/s12864-015-1637-7 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CK0QU UT WOS:000355910500001 PM 26040850 ER PT J AU Sarker, MI Fan, XT Liu, LS AF Sarker, Majher I. Fan, Xuetong Liu, LinShu TI Boron derivatives: As a source of 1-MCP with gradual release SO SCIENTIA HORTICULTURAE LA English DT Article DE Boron derivatives of methylene cyclopropane; 1-MCP; Plant growth regulator ID DIOXANE-MONOCHLOROBORANE; ETHYLENE BIOSYNTHESIS; 1-METHYLCYCLOPROPENE; TOMATO; REAGENT; HYDROBORATION; PERCEPTION; RECEPTORS; GREEN AB 1-Methylcycloprene (1-MCP) is a commercially available chemical used for the treatment of flowers or fruits to retard their ripening and thus prolong their shelf-life. The main difficulties associated are it's handling and control release on purpose as 1-MCP is a gas at ambient temperature, which limits its' broad spectrum of application. In this present study, we synthesized four boron derivatives-of methylene cyclopropane (MCP) which are stable at ambient conditions and capable of releasing 1-MCP gradually in contact with water depending on their reaction rates. These or similar compounds will offer better controlled delivery of 1-MCP than the existing technology, selective application, cost effective multi-batch treatment option and more importantly has the potential to be used in open fields to increase the crop yields around the globe. Open field application of 1-MCP can be a solution to protect crops from drought conditions, where higher crop yields are restricted due to inadequate amounts or unequal distribution of rain fall. Investigated complexes could be modified with the replacement of different alkyl or aryl groups and thus generating more complexes to explore. Published by Elsevier B.V. C1 [Sarker, Majher I.; Fan, Xuetong; Liu, LinShu] ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA,Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Liu, LS (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA,Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM LinShu.Liu@ARS.USDA.GOV NR 28 TC 0 Z9 0 U1 1 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUN 4 PY 2015 VL 188 BP 36 EP 43 DI 10.1016/j.scienta.2015.03.017 PG 8 WC Horticulture SC Agriculture GA CH6OO UT WOS:000354156100006 ER PT J AU Sapozhnikova, Y Lehotay, SJ AF Sapozhnikova, Yelena Lehotay, Steven J. TI Evaluation of Different Parameters in the Extraction of Incurred Pesticides and Environmental Contaminants in Fish SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE extractability; sample preparation; pesticides; environmental contaminants; fish; residue analysis ID TANDEM MASS-SPECTROMETRY; PRESSURE GAS-CHROMATOGRAPHY; POLYCYCLIC AROMATIC-HYDROCARBONS; POLYBROMINATED DIPHENYL ETHERS; SOLID-PHASE EXTRACTION; POLYCHLORINATED-BIPHENYLS; SAMPLE PREPARATION; RESIDUE ANALYSIS; LIQUID; QUECHERS AB Sample processing is often ignored during analytical method development and validation, but accurate results for real samples depend on all aspects of the analytical process. Also, validation is often conducted using only spiked samples, but extraction yields may be lower in incurred samples. In this study, different variables in extraction for incurred pesticides and environmental contaminants in fish were investigated. Among 207 analytes screened using low-pressure gas chromatography-tandem mass spectrometry, consisting of 150 pesticides, 15 polycyclic aromatic hydrocarbons (PAHs), 14 polychlorinated biphenyls (PCBs), 6 polybrominated diphenyl ethers (PBDEs), and 22 other flame retardants (FRs), 35 (16 pesticides, 9 PCBs, 5 PBDEs, and 5 PAHs) were identified for quantification in samples of salmon, croaker, and NIST Standard Reference Material 1947 (Lake Michigan Fish Tissue). Extraction efficiencies using different extraction devices (blending, vortexing, and vibrating) versus time, sample size, and sample/solvent ratio were determined. In comparison to blending results, use of a pulsed-vortexer for 1 min with 1/1 (g/mL) sample/acetonitrile ratio was generally sufficient to extract the incurred contaminants in the homogenized fish tissues. Conversely, extraction with a prototype vibration shaker often took 60 min to achieve 100% extraction efficiency. A main conclusion from this study is that accurate results for real samples can be obtained using batch extraction with a pulsed-vortexer in a simple and efficient method that achieves high sample throughput. C1 [Sapozhnikova, Yelena; Lehotay, Steven J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Steven.Lehotay@ars.usda.gov NR 19 TC 8 Z9 8 U1 6 U2 31 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 3 PY 2015 VL 63 IS 21 BP 5163 EP 5168 DI 10.1021/jf506256q PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CK0JG UT WOS:000355890900010 PM 25686151 ER PT J AU Venkatesh, TV Harrigan, GG Perez, T Flint-Garcia, S AF Venkatesh, Tyamagondlu V. Harrigan, George G. Perez, Tim Flint-Garcia, Sherry TI Compositional Assessments of Key Maize Populations: B73 Hybrids of the Nested Association Mapping Founder Lines and Diverse Landrace Inbred Lines SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE maize; landraces; Nested Association Mapping (NAM) founder lines; composition analysis ID ZEA-MAYS L.; GENETIC ARCHITECTURE; ARTIFICIAL SELECTION; GENOME; GRAIN; CORN; DETERMINANT; GERMPLASM; IMPACT AB The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America. The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits. Landraces are also of interest to the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines. The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (alpha-, gamma-, and delta-), beta-carotene, phytic acid, and raffinose. Grain was harvested from a replicated trial in New York, USA. For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set. Overall, results highlighted extensive variation in all composition components assessed for both sets of hybrids. The variation observed for some components within the landraces may therefore be of value for increasing their levels in modern maize lines. The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines. C1 [Venkatesh, Tyamagondlu V.; Harrigan, George G.; Perez, Tim] Monsanto Co, St Louis, MO 63167 USA. [Flint-Garcia, Sherry] ARS, USDA, Columbia, MO 65211 USA. [Flint-Garcia, Sherry] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Venkatesh, TV (reprint author), Monsanto Co, 800 N Lindbergh Blvd, St Louis, MO 63167 USA. EM t.v.venkatesh@monsanto.com; Sherry.Flint-Garcia@ars.usda.gov NR 41 TC 3 Z9 3 U1 2 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 3 PY 2015 VL 63 IS 21 BP 5282 EP 5295 DI 10.1021/acs.jafc.5b00208 PG 14 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CK0JG UT WOS:000355890900024 PM 25966324 ER PT J AU Schuller, S Sergeant, K Renaut, J Callanan, JJ Scaife, C Nally, JE AF Schuller, Simone Sergeant, Kjell Renaut, Jenny Callanan, John J. Scaife, Caitriona Nally, Jarlath E. TI Comparative proteomic analysis of lung tissue from guinea pigs with leptospiral pulmonary haemorrhage syndrome (LPHS) reveals a decrease in abundance of host proteins involved in cytoskeletal and cellular organization SO JOURNAL OF PROTEOMICS LA English DT Article DE Leptospiral pulmonary; haemorrhage syndrome; Leptospirosis; Guinea pig; DIGE; Lung proteome map ID DISSEMINATED INTRAVASCULAR COAGULATION; ALVEOLAR EPITHELIAL-CELLS; ACUTE-PHASE RESPONSE; ALPHA(1)-ACID GLYCOPROTEIN; INDUCIBLE EXPRESSION; MYCOBACTERIUM-BOVIS; BINDING PROTEIN; INTERROGANS; MODEL; THROMBOCYTOPENIA AB Leptospiral pulmonary haemorrhage syndrome (LPHS) is a particularly severe form of leptospirosis. LPHS is increasingly recognized in both humans and animals and is characterized by rapidly progressive intra-alveolar haemorrhage leading to high mortality. The pathogenic mechanisms of LPHS are poorly understood which hampers the application of effective treatment regimes. In this study a 2-D guinea pig proteome lung map was created and used to investigate the pathogenic mechanisms of LPHS. Comparison of lung proteomes from infected and non-infected guinea pigs via differential in-gel electrophoresis revealed highly significant differences in abundance of proteins contained in 130 spots. Acute phase proteins were the largest functional group amongst proteins with increased abundance in LPHS lung tissue, and likely reflect a local and/or systemic host response to infection. The observed decrease in abundance of proteins involved in cytoskeletal and cellular organization in LPHS lung tissue further suggests that infection with pathogenic Leptospira induces changes in the abundance of host proteins involved in cellular architecture and adhesion contributing to the dramatically increased alveolar septal wall permeability seen in LPHS. Biological significance The recent completion of the complete genome sequence of the guinea pig (Cavia porcellus) provides innovative opportunities to apply proteomic technologies to an important animal model of disease. In this study, the comparative proteomic analysis of lung tissue from experimentally infected guinea pigs with leptospiral pulmonary haemorrhage syndrome (LPHS) revealed a decrease in abundance of proteins involved in cellular architecture and adhesion, suggesting that loss or down-regulation of cytoskeletal and adhesion molecules plays an important role in the pathogenesis of LPHS. A publically available guinea pig lung proteome map was constructed to facilitate future pulmonary proteomics in this species. (C) 2015 Elsevier B.V. All rights reserved. C1 [Schuller, Simone; Callanan, John J.; Nally, Jarlath E.] Univ Coll Dublin, Sch Vet Med, Dublin 4, Ireland. [Sergeant, Kjell; Renaut, Jenny] Luxembourg Inst Sci & Technol, Environm Res & Innovat ERIN Dept, L-4422 Belvaux, Luxembourg. [Callanan, John J.; Scaife, Caitriona; Nally, Jarlath E.] Conway Inst Biomol & Biomed Res, Dublin 4, Ireland. [Schuller, Simone] Univ Bern, Vetsuisse Fac, CH-3012 Bern, Switzerland. [Callanan, John J.] Ross Univ, Sch Vet Med, Basseterre, St Kitts & Nevi. [Nally, Jarlath E.] Agr Res Serv, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, Dept Agr, Ames, IA 50010 USA. RP Schuller, S (reprint author), Univ Bern, Vetsuisse Fac, Langgassstr 128, CH-3012 Bern, Switzerland. EM simone.schuller@vetsuisse.unibe.ch RI Sergeant, Kjell/M-9789-2015 OI Sergeant, Kjell/0000-0003-2071-3510 FU Science Foundation Ireland [05/YI2/B696]; Department of Agriculture, Fisheries and Food [RSF06 363] FX This study was funded by the Science Foundation Ireland (05/YI2/B696) and the Research Stimulus Fund from the Department of Agriculture, Fisheries and Food (RSF06 363). The authors would like to acknowledge Mark Dalton, Alfie Redmond and the team at the UCD Biomedical Facility for helping us look after our experimental guinea pigs. We would also like to thank Prof. David McHugh and Dr. Kate Killick for their help with the Ingenuity Pathway Analysis. NR 76 TC 1 Z9 1 U1 3 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD JUN 3 PY 2015 VL 122 BP 55 EP 72 DI 10.1016/j.jprot.2015.03.021 PG 18 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CJ7WH UT WOS:000355710600005 PM 25818725 ER PT J AU Riley, RT Showker, JL Lee, CM Zipperer, CE Mitchell, TR Voss, KA Zitomer, NC Torres, O Matute, J Gregory, SG Ashley-Koch, AE Maddox, JR Gardner, N Gelineau-Van Waes, JB AF Riley, Ronald T. Showker, Jency L. Lee, Christine M. Zipperer, Cody E. Mitchell, Trevor R. Voss, Kenneth A. Zitomer, Nicholas C. Torres, Olga Matute, Jorge Gregory, Simon G. Ashley-Koch, Allison E. Maddox, Joyce R. Gardner, Nicole Gelineau-Van Waes, Janee B. TI A blood spot method for detecting fumonisin-induced changes in putative sphingolipid biomarkers in LM/Bc mice and humans SO FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT LA English DT Article DE corn; sphinganine 1-phosphate; maize; LC-MS; fumonisin; biomarkers; sphingolipid; blood spots ID NEURAL-TUBE DEFECTS; SPHINGOSINE 1-PHOSPHATE; METABOLISM; MAIZE; EXPOSURE; B-1; ERYTHROCYTES; GUATEMALA; CELLS; NEPHROTOXICITY AB Fumonisins (FB) are mycotoxins found in maize. They are hypothesised risk factors for neural tube defects (NTDs) in humans living where maize is a dietary staple. In LM/Bc mice, FB1-treatment of pregnant dams induces NTDs and results in increased levels of sphingoid base 1-phosphates in blood and tissues. The increased level of sphingoid base 1-phosphates in blood is a putative biomarker for FB1 inhibition of ceramide synthase in humans. Collection of blood spots on paper from finger sticks is a relatively non-invasive way to obtain blood for biomarker analysis. The objective of this study was to develop and validate in an animal model, and ultimately in humans, a method to estimate the volume of blood collected as blood spots on absorbent paper so as to allow quantification of the molar concentration of sphingoid base 1-phosphates in blood. To accomplish this objective, blood was collected from unexposed male LM/Bc and FB1-exposed pregnant LM/Bc mice and humans and applied to two types of absorbent paper. The sphingoid base 1-phosphates, absorbance at 270nm (A(270)), and total protein content (Bradford) were determined in the acetonitrile:water 5% formic acid extracts from the dried blood spots. The results show that in both mouse and human the A(270), total protein, and blood volume were closely correlated and the volume of blood spotted was accurately estimated using only the A(270) of the extracts. In mouse blood spots, as in tissues and embryos, the FB1-induced changes in sphingolipids were correlated with urinary FB1. The half-life of FB1 in the urine was short (<24h) and the elevation in sphingoid base 1-phosphates in blood was also short, although more persistent than the urinary FB1. C1 [Riley, Ronald T.; Showker, Jency L.; Lee, Christine M.; Zipperer, Cody E.; Mitchell, Trevor R.; Voss, Kenneth A.; Zitomer, Nicholas C.] ARS, Toxicol & Mycotoxin Res Unit, RB Russell Res Ctr, USDA, Athens, GA 30605 USA. [Torres, Olga; Matute, Jorge] Lab Diagnost Mol SA, Guatemala City, Guatemala. [Torres, Olga] Ctr Invest Nutr & Salud, Guatemala City, Guatemala. [Gregory, Simon G.; Ashley-Koch, Allison E.] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA. [Maddox, Joyce R.; Gardner, Nicole; Gelineau-Van Waes, Janee B.] Creighton Univ, Sch Med, Dept Pharmacol, Omaha, NE 68178 USA. RP Riley, RT (reprint author), ARS, Toxicol & Mycotoxin Res Unit, RB Russell Res Ctr, USDA, Athens, GA 30605 USA. EM ron.riley@ars.usda.gov OI Ashley-Koch, Allison/0000-0001-5409-9155 FU USDA-ARS [NP108, 6612-42000-012-00D]; Eunice Kennedy Shriver National Institute of Child Health & Human Development [RC4HD067971-01] FX This work was supported by USDA-ARS NP108 in house project 6612-42000-012-00D and Award Number RC4HD067971-01 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health & Human Development or the National Institutes of Health. NR 35 TC 3 Z9 3 U1 1 U2 15 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1944-0049 EI 1944-0057 J9 FOOD ADDIT CONTAM A JI Food Addit. Contam. Part A-Chem. PD JUN 3 PY 2015 VL 32 IS 6 BP 934 EP 949 DI 10.1080/19440049.2015.1027746 PG 16 WC Chemistry, Applied; Food Science & Technology; Toxicology SC Chemistry; Food Science & Technology; Toxicology GA CH5YP UT WOS:000354112400013 PM 25833119 ER PT J AU Matchett, JR Stark, PB Ostoja, SM Knapp, RA McKenny, HC Brooks, ML Langford, WT Joppa, LN Berlow, EL AF Matchett, J. R. Stark, Philip B. Ostoja, Steven M. Knapp, Roland A. McKenny, Heather C. Brooks, Matthew L. Langford, William T. Joppa, Lucas N. Berlow, Eric L. TI Detecting the influence of rare stressors on rare species in Yosemite National Park using a novel stratified permutation test SO SCIENTIFIC REPORTS LA English DT Article ID YELLOW-LEGGED FROG; NONNATIVE FISH; SIERRA-NEVADA; BUFO-CANORUS; CALIFORNIA; USA; CONSEQUENCES; VEGETATION; INTRODUCTIONS; REMOVAL AB Statistical models often use observational data to predict phenomena; however, interpreting model terms to understand their influence can be problematic. This issue poses a challenge in species conservation where setting priorities requires estimating influences of potential stressors using observational data. We present a novel approach for inferring influence of a rare stressor on a rare species by blending predictive models with nonparametric permutation tests. We illustrate the approach with two case studies involving rare amphibians in Yosemite National Park, USA. The endangered frog, Rana sierrae, is known to be negatively impacted by non-native fish, while the threatened toad, Anaxyrus canorus, is potentially affected by packstock. Both stressors and amphibians are rare, occurring in similar to 10% of potential habitat patches. We first predict amphibian occupancy with a statistical model that includes all predictors but the stressor to stratify potential habitat by predicted suitability. A stratified permutation test then evaluates the association between stressor and amphibian, all else equal. Our approach confirms the known negative relationship between fish and R. sierrae, but finds no evidence of a negative relationship between current packstock use and A. canorus breeding. Our statistical approach has potential broad application for deriving understanding (not just prediction) from observational data. C1 [Matchett, J. R.; Brooks, Matthew L.] US Geol Survey, Yosemite Field Stn, Oakhurst, CA 93644 USA. Univ Calif Berkeley, Dept Biostat, Berkeley, CA 94720 USA. [Ostoja, Steven M.] US Forest Serv, Sierra Natl Forest, Clovis, CA 93611 USA. [Knapp, Roland A.] Univ Calif, Sierra Nevada Aquat Res Lab, Mammoth Lakes, CA 93546 USA. [McKenny, Heather C.] Natl Pk Serv, Kantishna, AK 99755 USA. [Langford, William T.] RMIT Univ, Sch Math & Geospatial Sci, Melbourne, Vic 3000, Australia. [Joppa, Lucas N.] Microsoft Res, Sci Computat Lab, Cambridge CB1 2FB, England. [Berlow, Eric L.] Vibrant Data Inc, San Francisco, CA 94108 USA. RP Berlow, EL (reprint author), Vibrant Data Inc, 943 Clay St, San Francisco, CA 94108 USA. EM eric@mappr.io OI Stark, Philip/0000-0002-3771-9604 FU US Geological Survey Terrestrial, Freshwater, and Marine Ecosystems Program; Yosemite Conservancy; National Park Service; Process Minerals International FX We thank dozens of field assistants for their invaluable help with amphibian surveys for both R. sierrae and A. canorus. Mark Fincher, Liz Ballenger, Dan Abbe, and Travis Espinosa of YNP assisted with packstock data compilation and interpretation. The UC Merced Sierra Nevada Research Institute, the USGS Yosemite Field Station, the Sierra Nevada Aquatic Research Lab, the Swall Institute, Vibrant Data Labs, and Yosemite National Park all provided logistical support. Data compilation and analysis were supported by the US Geological Survey Terrestrial, Freshwater, and Marine Ecosystems Program. This project was also supported with funds from the Yosemite Conservancy and the National Park Service. W.T.L. is supported by a grant from Process Minerals International through Condition 13 of Australian EPBC Act approval 2010/5759. NR 37 TC 0 Z9 0 U1 3 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 2 PY 2015 VL 5 AR 10702 DI 10.1038/srep10702 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ6OC UT WOS:000355612200001 PM 26031755 ER PT J AU Diggins, CA Jachowski, DS Martin, J Ford, WM AF Diggins, Corinne A. Jachowski, David S. Martin, Jay Ford, W. Mark TI Incidental captures of Eastern Spotted Skunk in a High-elevation Red Spruce forest in Virginia SO NORTHEASTERN NATURALIST LA English DT Article ID SPILOGALE-PUTORIUS; NEOTOMA-MAGISTER; ARKANSAS AB Spilogale putorius (Eastern Spotted Skunk) is considered rare in the southern Appalachian Mountains and throughout much of its range. We report incidental captures of 6 Eastern Spotted Skunks in a high-elevation Picea rubens (Red Spruce) forest in southwestern Virginia during late February and March 2014. At 1520 m, these observations are the highest-elevation records for Eastern Spotted Skunk in the Appalachian Mountains. They are also the first known records of this species using Red Spruce forests in the southern Appalachians. C1 [Diggins, Corinne A.] Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Jachowski, David S.] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29634 USA. [Martin, Jay] US Forest Serv, George Washington Jefferson Natl Forest, Marion, VA 24354 USA. [Ford, W. Mark] US Geol Survey, Virginia Cooperat Fish & Wildlife Res Unit, Blacksburg, VA 24061 USA. RP Diggins, CA (reprint author), Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. EM cordie1@vt.edu NR 15 TC 1 Z9 1 U1 0 U2 0 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1092-6194 EI 1938-5307 J9 NORTHEAST NAT JI Northeast. Nat PD JUN PY 2015 VL 22 IS 2 BP N6 EP N10 DI 10.1656/045.022.0211 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8PI UT WOS:000370898300016 ER PT J AU Hibbitts, TJ Wahlberg, SA Voelker, G AF Hibbitts, Toby J. Wahlberg, Scott A. Voelker, Gary TI MXResolving the Identity of Texas Desmognathus SO SOUTHEASTERN NATURALIST LA English DT Article ID DUSKY SALAMANDERS; COASTAL-PLAIN; PLETHODONTIDAE; DECLINES; FROG AB Dusky salamanders in Texas have long been identified as Desmognathus auriculatus (Southern Dusky Salamander). However, recent taxonomic work has challenged this notion, restricting D. auriculatus to areas east of the Apalachicola River in Florida and Georgia, while all Texas populations are now considered D. conanti. Regrettably, Texas specimens were not included in the studies upon which these proposed taxonomic changes were based. Dusky salamanders have become increasingly difficult to find in Texas and have only been observed in the state 3 times since 1980. We surveyed 18 sites of which 6 were historical localities. We found 4 extant populations of dusky salamanders at new locations in Texas, one of which was a county record. We were unable to find dusky salamanders at some historically robust populations. We also used mtDNA sequence data to verify that Texas dusky salamanders are indeed D. conanti. C1 [Hibbitts, Toby J.; Voelker, Gary] Texas A&M Univ, Dept Wildlife & Fisheries Sci, Biodivers Res & Teaching Collect, College Stn, TX 77843 USA. [Wahlberg, Scott A.] US Forest Serv, USDA, Southern Res Stn, Nacogdoches, TX 75965 USA. RP Hibbitts, TJ (reprint author), Texas A&M Univ, Dept Wildlife & Fisheries Sci, Biodivers Res & Teaching Collect, College Stn, TX 77843 USA. EM thibbitts@tamu.edu NR 13 TC 1 Z9 1 U1 0 U2 0 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD JUN PY 2015 VL 14 IS 2 BP 213 EP 220 PG 8 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DF1MX UT WOS:000371105000001 ER PT J AU Doumas, SL Koprowski, JL Noble, WO AF Doumas, Sandra L. Koprowski, John L. Noble, William O. TI LANDSCAPE-LEVEL ASSESSMENT OF ABERT'S SQUIRREL AND RED SQUIRREL IN MIXED CONIFER FOREST SO SOUTHWESTERN NATURALIST LA English DT Article ID PONDEROSA PINE; INTRODUCED POPULATION; SCIURUS-ABERTI; TAMIASCIURUS; RESPONSES; ARIZONA; SITES AB We examined feeding sign of Abert's squirrels (Sciurus aberti) and red squirrels (Tamiasciurus hudsonicus) each spring 2005-2009 in mixed conifer forests in northern Arizona, USA. We used high-resolution aerial photographs to assess vegetation characteristics where feeding sign surveys were conducted and analyzed the association of feeding sign frequency to vegetation characteristics to compare and contrast species habitat use. At the landscape level, we found considerable overlap in characteristics of forests used by the two species of native tree squirrels. Red squirrels used denser forests with fewer and smaller gaps than do Abert's squirrels, and Abert's squirrels used more ponderosa pine (Pinus ponderosa) than do red squirrels. C1 [Doumas, Sandra L.; Koprowski, John L.] Univ Arizona, Sch Nat Resources & Environm, Wildlife Conservat & Management, Tucson, AZ 85721 USA. [Noble, William O.] Natl Forest Serv, USDA, Williams, AZ 86046 USA. RP Doumas, SL (reprint author), Univ Arizona, Sch Nat Resources & Environm, Wildlife Conservat & Management, Tucson, AZ 85721 USA. EM sdoumas@email.arizona.edu FU National Natural Landmark Program of the National Park Service FX We thank the National Natural Landmark Program of the National Park Service for funding and H. Eggleston (National Park Service), D. Hanni, and D. Pavlacky (Rocky Mountain Bird Observatory) for assistance. NR 33 TC 0 Z9 0 U1 1 U2 5 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 EI 1943-6262 J9 SOUTHWEST NAT JI Southw. Natural. PD JUN-SEP PY 2015 VL 60 IS 2-3 BP 240 EP 246 DI 10.1894/SWNAT-D-14-00016.1 PG 7 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NR UT WOS:000370893300014 ER PT J AU Elmqvist, T Setala, H Handel, SN van der Ploeg, S Aronson, J Blignaut, JN Gomez-Baggethun, E Nowak, DJ Kronenberg, J de Groot, R AF Elmqvist, T. Setala, H. Handel, S. N. van der Ploeg, S. Aronson, J. Blignaut, J. N. Gomez-Baggethun, E. Nowak, D. J. Kronenberg, J. de Groot, R. TI Benefits of restoring ecosystem services in urban areas SO CURRENT OPINION IN ENVIRONMENTAL SUSTAINABILITY LA English DT Article ID GREEN SPACE; AIR-POLLUTION; HUMAN HEALTH; ENVIRONMENT; LANDSCAPES; ECOLOGY; URBANIZATION; RESTORATION; DISSERVICES; COMPLEXITY AB Cities are a key nexus of the relationship between people and nature and are huge centers of demand for ecosystem services and also generate extremely large environmental impacts. Current projections of rapid expansion of urban areas present fundamental challenges and also opportunities to design more livable, healthy and resilient cities (e.g. adaptation to climate change effects). We present the results of an analysis of benefits of ecosystem services in urban areas. Empirical analyses included estimates of monetary benefits from urban ecosystem services based on data from 25 urban areas in the USA, Canada, and China. Our results show that investing in ecological infrastructure in cities, and the ecological restoration and rehabilitation of ecosystems such as rivers, lakes, and woodlands occurring in urban areas, may not only be ecologically and socially desirable, but also quite often, economically advantageous, even based on the most traditional economic approaches. C1 [Elmqvist, T.] Stockholm Univ, Stockholm Resilience Ctr, SE-10691 Stockholm, Sweden. [Setala, H.] Univ Helsinki, Dept Environm Sci, FIN-15140 Lahti, Finland. [Handel, S. N.] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. [van der Ploeg, S.; de Groot, R.] Wageningen Univ, Environm Syst Anal Grp, NL-6700 AA Wageningen, Netherlands. [Aronson, J.] CEFE, UMR 5175, F-34293 Montpellier, France. [Aronson, J.] Missouri Bot Garden, St Louis, MO 63110 USA. [Blignaut, J. N.] Univ Pretoria, Dept Econ, ZA-0002 Pretoria, South Africa. [Gomez-Baggethun, E.] Norwegian Inst Nat Res NINA, N-0349 Oslo, Norway. [Gomez-Baggethun, E.] Autonomous Univ Barcelona, Inst Environm Sci & Technol ICTA, Barcelona, Spain. [Nowak, D. J.] SUNY ESF, USDA Forest Serv, Syracuse, NY 13210 USA. [Kronenberg, J.] Univ Lodz, Fac Econ & Sociol, PL-90255 Lodz, Poland. RP Elmqvist, T (reprint author), Stockholm Univ, Stockholm Resilience Ctr, SE-10691 Stockholm, Sweden. EM thomas.elmqvist@su.se OI Kronenberg, Jakub/0000-0003-4903-2401; Setala, Heikki Martti/0000-0002-5230-4001 FU Formas and Biodiversa through the URBES project; GREEN SURGE, EU FP7 collaborative project [FP7-ENV.2013.6.2-5-603567] FX We thank the TEEB team and team leader Pavan Sukhdev for inspiring discussions and crucial views. This study has been possible through support to T. Elmqvist from Formas and Biodiversa through the URBES project and to T. Elmqvist and J. Kronenberg by GREEN SURGE, EU FP7 collaborative project, FP7-ENV.2013.6.2-5-603567. NR 81 TC 14 Z9 16 U1 30 U2 88 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1877-3435 EI 1877-3443 J9 CURR OPIN ENV SUST JI Curr. Opin. Environ. Sustain. PD JUN PY 2015 VL 14 BP 101 EP 108 DI 10.1016/j.cosust.2015.05.001 PG 8 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA CY3SP UT WOS:000366330500013 ER PT J AU Huang, XD Shupe, TF Hse, CY AF Huang, Xiao-dong Shupe, Todd F. Hse, Chung-Yun TI Study of moso bamboo's permeability and mechanical properties SO EMERGING MATERIALS RESEARCH LA English DT Article DE material characterization; material processing; materials science AB In this article, moso bamboo blocks were first treated with hydrochloric acid solvents with different concentrations or microwave treatments with various microwave output power and treatment durations. The results showed that the crystalliferous region of cellulose of moso bamboo blocks formed porous or swelling type structures under hydrochloric acid pretreatment conditions; the mechanical strength of the moso bamboo decreased when the percentage of hydrochloric acid pretreatment solvents increased from 0 to 3%. When the moso bamboo blocks were treated with microwave, the internal water was transformed into steam, and the water vapor destroyed the cell walls of the vessel, sieve tube, and parenchyma cells in the moso bamboo block, resulting in a significant improvement in the permeability of moso bamboo. In order to ensure that the moso bamboo samples did not burn by microwave heating, the samples had to be immersed in water. The process of water gasification inside the Moso bamboo was not violent. Therefore, the mechanical strength of the moso bamboo had decreased only slightly, and a weak relationship was found between the mechanical strength of the moso bamboo samples and the microwave treatment durations. C1 [Huang, Xiao-dong] Fujian Agr & Forestry Univ, Coll Mat Engn, Fuzhou, Peoples R China. [Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Nat Renewable Resource, Baton Rouge, LA 70803 USA. [Hse, Chung-Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA USA. RP Huang, XD (reprint author), Fujian Agr & Forestry Univ, Coll Mat Engn, Fuzhou, Peoples R China. EM Xiaodong_h@163.com FU Fujian Provincial Department of Science and Technology, China [2014 K53NI904A] FX The authors thank the Southern Research Station, USDA Forest Service, for the supplements in this research. The authors are grateful for the financial support of the Fujian Provincial Department of Science and Technology, China (2014 K53NI904A). NR 11 TC 0 Z9 0 U1 3 U2 7 PU ICE PUBLISHING PI WESTMINISTER PA INST CIVIL ENGINEERS, 1 GREAT GEORGE ST, WESTMINISTER SW 1P 3AA, ENGLAND SN 2046-0147 EI 2046-0155 J9 EMERG MATER RES JI Emerg. Mater. Res. PD JUN PY 2015 VL 4 IS 1 BP 130 EP 138 DI 10.1680/emr.14.00034 PG 9 WC Materials Science, Multidisciplinary SC Materials Science GA CX5CS UT WOS:000365719700018 ER PT J AU Miller, AJ Novy, A Glover, J Kellogg, EA Maul, JE Raven, P Jackson, PW AF Miller, A. J. Novy, A. Glover, J. Kellogg, E. A. Maul, J. E. Raven, P. Jackson, P. Wyse TI Expanding the role of botanical gardens in the future of food SO NATURE PLANTS LA English DT Editorial Material C1 [Miller, A. J.] St Louis Univ, Dept Biol, St Louis, MO 63103 USA. [Miller, A. J.; Raven, P.; Jackson, P. Wyse] Missouri Bot Garden, St Louis, MO 63110 USA. [Novy, A.] US Bot Garden, Washington, DC 20024 USA. [Novy, A.] Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, Washington, DC 20560 USA. [Glover, J.] US Agcy Int Dev, Washington, DC 20004 USA. [Kellogg, E. A.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Maul, J. E.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Jackson, P. Wyse] Washington Univ, Dept Biol, St Louis, MO 63130 USA. RP Miller, AJ (reprint author), St Louis Univ, Dept Biol, St Louis, MO 63103 USA. EM amille75@slu.edu; anovy@aoc.gov RI Kellogg, Elizabeth/M-2845-2013 OI Kellogg, Elizabeth/0000-0003-1671-7447 NR 14 TC 0 Z9 0 U1 2 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD JUN 1 PY 2015 VL 1 IS 6 AR 15078 DI 10.1038/NPLANTS.2015.78 PG 4 WC Plant Sciences SC Plant Sciences GA CV6TI UT WOS:000364403300001 PM 27250013 ER PT J AU Jackson, CR Stone, BWG Tyler, HL AF Jackson, Colin R. Stone, Bram W. G. Tyler, Heather L. TI Emerging Perspectives on the Natural Microbiome of Fresh Produce Vegetables SO AGRICULTURE-BASEL LA English DT Review DE microbiome; bacteria; salad produce; herbs; phyllosphere; endophytes; 16S rRNA ID ESCHERICHIA-COLI O157-H7; PHYLLOSPHERE BACTERIAL COMMUNITIES; SUBSTRATE UTILIZATION PATTERNS; SALMONELLA-ENTERICA SEROVARS; GRADIENT GEL-ELECTROPHORESIS; PEPPER PLANT PHYLLOSPHERE; RIBOSOMAL-RNA; HUMAN FECES; IRRIGATION WATER; ALFALFA SPROUTS AB Plants harbor a diverse microbiome existing as bacterial populations on the leaf surface (the phyllosphere) and within plant tissues (endophytes). The composition of this microbiome has been largely unexplored in fresh produce vegetables, where studies have tended to focus on pathogen detection and survival. However, the application of next-generation 16S rRNA gene sequencing approaches is beginning to reveal the diversity of this produce-associated bacterial community. In this article we review what is known about the composition of the microbiome of fresh produce vegetables, placing it in the context of general phyllosphere research. We also demonstrate how next-generation sequencing can be used to assess the bacterial assemblages present on fresh produce, using fresh herbs as an example. That data shows how the use of such culture-independent approaches can detect groups of taxa (anaerobes, psychrophiles) that may be missed by traditional culture-based techniques. Other issues discussed include questions as to whether to determine the microbiome during plant growth or at point of purchase or consumption, and the potential role of the natural bacterial community in mitigating pathogen survival. C1 [Jackson, Colin R.; Stone, Bram W. G.] Univ Mississippi, Dept Biol, University, MS 38677 USA. [Tyler, Heather L.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Jackson, CR (reprint author), Univ Mississippi, Dept Biol, University, MS 38677 USA. EM cjackson@olemiss.edu; bstone2@go.olemiss.edu; Heather.Tyler@ars.usda.gov NR 63 TC 2 Z9 2 U1 8 U2 18 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD JUN PY 2015 VL 5 IS 2 BP 170 EP 187 DI 10.3390/agriculture5020170 PG 18 WC Agronomy SC Agriculture GA CV4HL UT WOS:000364227700002 ER PT J AU Williams, WP Windham, GL AF Williams, W. Paul Windham, Gary L. TI Aflatoxin Accumulation in a Maize Diallel Cross SO AGRICULTURE-BASEL LA English DT Article DE Aspergillus flavus; aflatoxin; maize; plant resistance ID ASPERGILLUS-FLAVUS; GERMPLASM LINE; INSECT DAMAGE; REGISTRATION; CORN; CONTAMINATION; RESISTANCE; MYCOTOXINS; GEORGIA; MP313E AB Aflatoxins, produced by the fungus Aspergillus flavus, occur naturally in maize. Contamination of maize grain with aflatoxin is a major food and feed safety problem and greatly reduces the value of the grain. Plant resistance is generally considered a highly desirable approach to reduction or elimination of aflatoxin in maize grain. In this investigation, a diallel cross was produced by crossing 10 inbred lines with varying degrees of resistance to aflatoxin accumulation in all possible combinations. Three lines that previously developed and released as sources of resistance to aflatoxin accumulation were included as parents. The 10 parental inbred lines and the 45 single crosses making up the diallel cross were evaluated for aflatoxin accumulation in field tests conducted in 2013 and 2014. Plants were inoculated with an A. flavus spore suspension seven days after silk emergence. Ears were harvested approximately 60 days later and concentration of aflatoxin in the grain determined. Parental inbred lines Mp717, Mp313E, and Mp719 exhibited low levels (3-12 ng/g) of aflatoxin accumulation. In the diallel analysis, both general and specific combining ability were significant sources of variation in the inheritance of resistance to aflatoxin accumulation. General combining ability effects for reduced aflatoxin accumulation were greatest for Mp494, Mp719, and Mp717. These lines should be especially useful in breeding for resistance to aflatoxin accumulation. Breeding strategies, such as reciprocal recurrent selection, would be appropriate. C1 [Williams, W. Paul; Windham, Gary L.] Agr Res Serv, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. RP Williams, WP (reprint author), Agr Res Serv, USDA, Corn Host Plant Resistance Res Unit, POB 9555, Mississippi State, MS 39762 USA. EM paul.williams@ars.usda.gov; gary.windham@ars.usda.gov NR 26 TC 0 Z9 0 U1 0 U2 1 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD JUN PY 2015 VL 5 IS 2 BP 344 EP 352 DI 10.3390/agriculture5020344 PG 9 WC Agronomy SC Agriculture GA CV4HL UT WOS:000364227700011 ER PT J AU Tirado-Corbala, R Anderson, RG Wang, D Ayars, JE AF Tirado-Corbala, Rebecca Anderson, Ray G. Wang, Dong Ayars, James E. TI Soil Carbon and Nitrogen Stocks of Different Hawaiian Sugarcane Cultivars SO AGRONOMY-BASEL LA English DT Article DE Hawaii sugarcane; cultivars; soil carbon; soil nitrogen; carbon sequestration; biofuel ID IRRIGATED SUGARCANE; FRACTIONATION; MANAGEMENT; BIOFUELS; SYSTEM; BRAZIL; CROPS; SEQUESTRATION; ACCUMULATION; HARVEST AB Sugarcane has been widely used as a biofuel crop due to its high biological productivity, ease of conversion to ethanol, and its relatively high potential for greenhouse gas reduction and lower environmental impacts relative to other derived biofuels from traditional agronomic crops. In this investigation, we studied four sugarcane cultivars ( H- 65- 7052, H- 78- 3567, H- 86- 3792 and H- 87- 4319) grown on a Hawaiian commercial sugarcane plantation to determine their ability to store and accumulate soil carbon ( C) and nitrogen ( N) across a 24- month growth cycle on contrasting soil types. The main study objective establish baseline parameters for biofuel production life cycle analyses; sub- objectives included ( 1) determining which of four main sugarcane cultivars sequestered the most soil C and ( 2) assessing how soil C sequestration varies among two common Hawaiian soil series ( Pulehu- sandy clay loam and Molokai- clay). Soil samples were collected at 20 cm increments to depths of up to 120 cm using hand augers at the three main growth stages ( tillering, grand growth, and maturity) from two experimental plots at to observe total carbon ( TC), total nitrogen ( TN), dissolved organic carbon ( DOC) and nitrates ( NO- 3) using laboratory flash combustion for TC and TN and solution filtering and analysis for DOC and NO- 3. Aboveground plant biomass was collected and subsampled to determine lignin and C and N content. This study determined that there was an increase of TC with the advancement of growing stages in the studied four sugarcane cultivars at both soil types ( increase in TC of 15- 35 kg center dot m2). Nitrogen accumulation was more variable, and NO- 3 (< 5 ppm) were insignificant. The C and N accumulation varies in the whole profile based on the ability of the sugarcane cultivar's roots to explore and grow in the different soil types. For the purpose of storing C in the soil, cultivar H- 65- 7052 ( TC accumulation of similar to 30 kg center dot m- 2) and H- 86- 3792 ( 25 kg center dot m- 2) rather H- 78- 3567 ( 15 kg center dot m- 2) and H- 87- 4319 ( 20 kg center dot m- 2) appeared to produce more accumulated carbon in both soil types. C1 [Tirado-Corbala, Rebecca; Anderson, Ray G.; Wang, Dong; Ayars, James E.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, Parlier, CA 93648 USA. [Tirado-Corbala, Rebecca] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR USA. [Anderson, Ray G.] USDA ARS, US Salin Lab Contaminant Fate & Transport Unit, Riverside, CA 92507 USA. RP Tirado-Corbala, R (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM rebeccatiradocorbala@gmail.com; ray.anderson@ars.usda.gov; dong.wang@ars.usda.gov; james.ayars@ars.usda.gov OI Anderson, Ray/0000-0002-6202-5890 NR 71 TC 3 Z9 3 U1 2 U2 4 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD JUN PY 2015 VL 5 IS 2 BP 239 EP 261 DI 10.3390/agronomy5020239 PG 23 WC Agronomy SC Agriculture GA CV4HK UT WOS:000364227500009 ER PT J AU Chang, ML Chiu, YH Chiu, CJ Avoni, L AF Chang, Min-Lee Chiu, Yu-Hsuan Chiu, Chung-Jung Avoni, Luca TI Mobile application software (App) for Prediction of Advanced Age-related Macular Degeneration SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Chang, Min-Lee; Chiu, Chung-Jung] Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chiu, Yu-Hsuan] Concord Carlisle High Sch, Concord, MA USA. [Chiu, Chung-Jung] Tufts Univ, Sch Med, Dept Ophthalmol, Boston, MA 02111 USA. [Avoni, Luca] Bologna Hosp Osped Maggiore, Dept Ophthalmol, Bologna, Italy. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 4111 PG 2 WC Ophthalmology SC Ophthalmology GA CT5ZY UT WOS:000362891102312 ER PT J AU Chiu, CJ Chang, ML Li, T Gensler, G Taylor, A AF Chiu, Chung-Jung Chang, Min-Lee Li, Tricia Gensler, Gary Taylor, Allen TI American Minor Dietary Patterns and Age-related Macular Degeneration SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Chiu, Chung-Jung; Chang, Min-Lee; Taylor, Allen] Tufts Univ, JM USDA Human Nutr Res Ctr, Boston, MA 02111 USA. [Chiu, Chung-Jung; Taylor, Allen] Tufts Univ, Sch Med, Dept Ophthalmol, Boston, MA 02111 USA. [Li, Tricia] Harvard Univ, Brigham & Womens Hosp, Channing Div Network Med, Sch Med, Boston, MA 02115 USA. [Gensler, Gary] EMMES Corp, Age Related Eye Dis Study Coordinating Ctr, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 3759 PG 2 WC Ophthalmology SC Ophthalmology GA CT5ZY UT WOS:000362891101384 ER PT J AU Whitcomb, E Chang, ML Taylor, A AF Whitcomb, Elizabeth Chang, Min-Lee Taylor, Allen TI The Ubiquitin Conjugating Enzyme, UbcH7, Affects Both Migration and the Epithelial to Mesenchymal Transition in Lens Epithelial Cells: A Promising Drug Target for Secondary Cataracts SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Whitcomb, Elizabeth; Chang, Min-Lee; Taylor, Allen] Tufts Univ, HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 3213 PG 2 WC Ophthalmology SC Ophthalmology GA CT5ZY UT WOS:000362891100088 ER PT J AU Adenipekun, EO Jackson, CR Oluwadun, A Iwalokun, BA Frye, JG Barrett, JB Hiott, LM Woodley, TA AF Adenipekun, Eyitayo O. Jackson, Charlene R. Oluwadun, Afolabi Iwalokun, Bamidele A. Frye, Jonathan G. Barrett, John B. Hiott, Lari M. Woodley, Tiffanie A. TI Prevalence and Antimicrobial Resistance in Escherichia coli from Food Animals in Lagos, Nigeria SO MICROBIAL DRUG RESISTANCE LA English DT Article ID FIELD GEL-ELECTROPHORESIS; SPECTRUM BETA-LACTAMASES; ANTIBIOTIC-RESISTANCE; MULTIDRUG-RESISTANCE; ENVIRONMENT; SALMONELLA; POULTRY; STRAINS; PLASMID; HUMANS AB Foodborne bacteria are often associated with human infections; these infections can become more complicated to treat if the bacteria are also resistant to antimicrobials. In this study, prevalence, antimicrobial resistance, and genetic relatedness of Escherichia coli among food producing animals from Lagos, Nigeria, was investigated. From December 2012 to June 2013, E. coli were isolated from fecal samples of healthy cattle, chicken, and swine. Antimicrobial susceptibility testing against 22 antimicrobials was performed using broth microdilution with the Sensititre system. Clonal types were determined by pulsed-field gel electrophoresis (PFGE). From the analysis, 211/238 (88.7%), 170/210 (81%), and 136/152 (89.5%) samples from cattle, chicken, and swine, respectively, were positive for E. coli. A subset of those isolates (n=211) selected based on -lactamase production was chosen for further study. Overall, E. coli exhibited the highest resistance to tetracycline (124/211; 58.8%), trimethoprim/sulfamethoxazole (84/211; 39.8%), and ampicillin (72/211; 34.1%). Approximately 40% of the isolates were pan-susceptible, and none of the isolates were resistant to amikacin, cefepime, ceftazidime, ertapenem, meropenem, or tigecycline. Among the resistant isolates, 28 different resistance patterns were observed; 26 of those were characterized as multi-drug resistant (MDR; resistance to 2 antimicrobials). One isolate was resistant to 13 different antimicrobials representing five different antimicrobial classes. Using PFGE, MDR E. coli were genetically diverse and overall did not group based on source; identical PFGE patterns were detected among isolates from different sources. These results suggest that isolates cannot be attributed to specific sources, and some may be present across all of the sources. Results from this study indicate that food-producing animals in Nigeria are a reservoir of MDR E. coli that may be transferred to humans via the food chain. C1 [Adenipekun, Eyitayo O.; Oluwadun, Afolabi; Iwalokun, Bamidele A.] Olabisi Onabanjo Univ, Coll Hlth Sci, Dept Med Microbiol & Parasitol, Shagamu, Nigeria. [Jackson, Charlene R.; Frye, Jonathan G.; Barrett, John B.; Hiott, Lari M.; Woodley, Tiffanie A.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Res Ctr, Athens, GA 30605 USA. [Iwalokun, Bamidele A.] Nigerian Inst Med Res, Dept Biochem, Lagos, Nigeria. RP Jackson, CR (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM charlene.jackson@ars.usda.gov OI Frye, Jonathan/0000-0002-8500-3395 NR 37 TC 2 Z9 2 U1 0 U2 5 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 EI 1931-8448 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD JUN 1 PY 2015 VL 21 IS 3 BP 358 EP 365 DI 10.1089/mdr.2014.0222 PG 8 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA CU9PB UT WOS:000363875600016 PM 25658418 ER PT J AU Gay, CG AF Gay, Cyril G. TI Orbiviruses: A Gap Analysis SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Editorial Material C1 ARS, Anim Prod & Protect, Off Natl Programs, USDA, Beltsville, MD 20705 USA. RP Gay, CG (reprint author), ARS, Anim Prod & Protect, Off Natl Programs, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 333 EP 334 DI 10.1089/vbz.2015.28999.cgg PG 2 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200001 PM 26086553 ER PT J AU McVey, DS Drolet, BS Ruder, MG Wilson, WC Nayduch, D Pfannenstiel, R Cohnstaedt, LW MacLachlan, NJ Gay, CG AF McVey, D. Scott Drolet, Barbara S. Ruder, Mark G. Wilson, William C. Nayduch, Dana Pfannenstiel, Robert Cohnstaedt, Lee W. MacLachlan, N. James Gay, Cyril G. TI Orbiviruses: A North American Perspective SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Bluetongue virus; Epizootic hemorrhagic disease; Vector; Vaccine; Diagnostic test; Epidemiology ID EPIZOOTIC HEMORRHAGIC-DISEASE; BLUETONGUE-VIRUS SEROTYPE-2; UNITED-STATES; PHYLOGENETIC ANALYSIS; MEDITERRANEAN BASIN; GENETIC DIVERSITY; EUROPE; SEQUENCE; STRAINS; CLIMATE AB Orbiviruses are members of the Reoviridae family and include bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV). These viruses are the cause of significant regional disease outbreaks among livestock and wildlife in the United States, some of which have been characterized by significant morbidity and mortality. Competent vectors are clearly present in most regions of the globe; therefore, all segments of production livestock are at risk for serious disease outbreaks. Animals with subclinical infections also serve as reservoirs of infection and often result in significant trade restrictions. The economic and explicit impacts of BTV and EHDV infections are difficult to measure, but infections are a cause of economic loss for producers and loss of natural resources (wildlife). In response to United States Animal Health Association (USAHA) Resolution 16, the US Department of Agriculture (USDA), in collaboration with the Department of the Interior (DOI), organized a gap analysis workshop composed of international experts on Orbiviruses. The workshop participants met at the Arthropod-Borne Animal Diseases Research Unit in Manhattan, KS, May 14-16, 2013, to assess the available scientific information and status of currently available countermeasures to effectively control and mitigate the impact of an outbreak of an emerging Orbivirus with epizootic potential, with special emphasis given to BTV and EHDV. In assessing the threats, workshop participants determined that available countermeasures are somewhat effective, but several weaknesses were identified that affect their ability to prevent and control disease outbreaks effectively. C1 [McVey, D. Scott; Drolet, Barbara S.; Ruder, Mark G.; Wilson, William C.; Nayduch, Dana; Pfannenstiel, Robert; Cohnstaedt, Lee W.] ARS, USDA, ABADRU, Manhattan, KS 66502 USA. [MacLachlan, N. James] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA. [Gay, Cyril G.] ARS, USDA, Natl Program Anim Hlth 103, Beltsville, MD USA. RP McVey, DS (reprint author), ARS, USDA, ABADRU, 115 Coll Ave, Manhattan, KS 66502 USA. EM scott.mcvey@ars.usda.gov NR 48 TC 1 Z9 1 U1 3 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 335 EP 338 DI 10.1089/vbz.2014.1699 PG 4 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200002 PM 26086554 ER PT J AU Drolet, BS van Rijn, P Howerth, EW Beer, M Mertens, PP AF Drolet, Barbara S. van Rijn, Piet Howerth, Elizabeth W. Beer, Martin Mertens, Peter P. TI A Review of Knowledge Gaps and Tools for Orbivirus Research SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Review DE Orbivirus; Bluetongue virus; Epizootic hemorrhagic disease virus; Culicoides; Midges; Review ID EPIZOOTIC-HEMORRHAGIC-DISEASE; BLUETONGUE-VIRUS SEROTYPE; WHITE-TAILED DEER; SOUTH-EAST ASIA; IMMUNE-RESPONSE; GENETIC-CHARACTERIZATION; CULICOIDES-VARIIPENNIS; PHYLOGENETIC ANALYSIS; TOGGENBURG ORBIVIRUS; INTERFERON RECEPTOR AB Although recognized as causing emerging and re-emerging disease outbreaks worldwide since the late 1800s, there has been growing interest in the United States and Europe in recent years in orbiviruses, their insect vectors, and the diseases they cause in domestic livestock and wildlife. This is due, in part, to the emergence of bluetongue (BT) in northern Europe in 2006-2007 resulting in a devastating outbreak, as well as severe BT outbreaks in sheep and epizootic hemorrhagic disease (EHD) outbreaks in deer and cattle in the United States. Of notable concern is the isolation of as many as 10 new BT virus (BTV) serotypes in the United States since 1999 and their associated unknowns, such as route of introduction, virulence to mammals, and indigenous competent vectors. This review, based on a gap analysis workshop composed of international experts on orbiviruses conducted in 2013, gives a global perspective of current basic virological understanding of orbiviruses, with particular attention to BTV and the closely related epizootic hemorrhagic disease virus (EHDV), and identifies a multitude of basic virology research gaps, critical for predicting and preventing outbreaks. C1 [Drolet, Barbara S.] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66535 USA. [van Rijn, Piet] Wageningen Univ, Cent Vet Inst, Dept Virol, NL-6700 AP Wageningen, Netherlands. [van Rijn, Piet] North West Univ, Ctr Human Metabon, Dept Biochem, Potchefstroom, South Africa. [Howerth, Elizabeth W.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. [Beer, Martin] Friedrich Loeffler Inst, Inst Diagnost Virol, Insel Riems, Germany. [Mertens, Peter P.] Pirbright Inst, Vector Borne Dis Programme, Woking, Surrey, England. RP Drolet, BS (reprint author), ARS, USDA, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66535 USA. EM barbara.drolet@ars.usda.gov RI Institute, Pirbright/K-4476-2014; OI Mertens, Peter/0000-0002-3438-3738; van Rijn, Piet A./0000-0002-2594-1232 NR 94 TC 1 Z9 1 U1 3 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 339 EP 347 DI 10.1089/vbz.2014.1701 PG 9 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200003 PM 26086555 ER PT J AU Ruder, MG Lysyk, TJ Stallknecht, DE Foil, LD Johnson, DJ Chase, CC Dargatz, DA Gibbs, EPJ AF Ruder, Mark G. Lysyk, Timothy J. Stallknecht, David E. Foil, Lane D. Johnson, Donna J. Chase, Christopher C. Dargatz, David A. Gibbs, E. Paul J. TI Transmission and Epidemiology of Bluetongue and Epizootic Hemorrhagic Disease in North America: Current Perspectives, Research Gaps, and Future Directions SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Bluetongue virus; Culicoides; Epidemiology; Epizootic hemorrhagic disease virus; Transmission; Vector ID WHITE-TAILED DEER; CULICOIDES-SONORENSIS DIPTERA; VARIIPENNIS COMPLEX DIPTERA; SOUTHERN CALIFORNIA DAIRY; VIRUS-INFECTED CATTLE; UNITED-STATES; ODOCOILEUS-VIRGINIANUS; MOLECULAR CHARACTERIZATION; ANAPLASMA-MARGINALE; ENZOOTIC STABILITY AB Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are arthropod-transmitted viruses in the genus Orbivirus of the family Reoviridae. These viruses infect a variety of domestic and wild ruminant hosts, although the susceptibility to clinical disease associated with BTV or EHDV infection varies greatly among host species, as well as between individuals of the same species. Since their initial detection in North America during the 1950s, these viruses have circulated in endemic and epidemic patterns, with occasional incursions to more northern latitudes. In recent years, changes in the pattern of BTV and EHDV infection and disease have forced the scientific community to revisit some fundamental areas related to the epidemiology of these diseases, specifically in relation to virus-vector-host interactions and environmental factors that have potentially enabled the observed changes. The aim of this review is to identify research and surveillance gaps that obscure our understanding of BT and EHD in North America. C1 [Ruder, Mark G.] ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS 66502 USA. [Lysyk, Timothy J.] Agr & Agri Food Canada, Res Ctr, Lethbridge, AB, Canada. [Stallknecht, David E.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA USA. [Foil, Lane D.] Louisiana State Univ, Agctr, Bob Jones Wildlife Res Inst, Idlewild, LA USA. [Johnson, Donna J.] US Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, STAS, Vet Serv,USDA, Ames, IA USA. [Chase, Christopher C.] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. [Dargatz, David A.] US Anim & Plant Hlth Inspect Serv, Ctr Epidemiol & Anim Hlth, STAS, Vet Serv,USDA, Ft Collins, CO USA. [Gibbs, E. Paul J.] Univ Florida, Coll Vet Med, Dept Infect Dis, Gainesville, FL USA. RP Ruder, MG (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, USDA, 1515 Coll Ave, Manhattan, KS 66502 USA. EM mark.ruder@ars.usda.gov FU US Department of Agriculture (USDA) Agricultural Research Service (ARS), National Program for Animal Health FX We acknowledge the organizing committee and the US Department of Agriculture (USDA) Agricultural Research Service (ARS), National Program for Animal Health for sponsoring and conducting an Orbivirus Research Symposium during May, 2013, in Manhattan, Kansas, at the Center for Grain and Animal Health Research (CGAHR, USDA-ARS). The authors were grateful to participate and thank all of the participants for donating their time and talent. This paper will be part of the report from that symposium. The authors also thank the staff of the Arthropod-Borne Animal Diseases Research Unit and the CGAHR for on-site support of the symposium. NR 155 TC 4 Z9 4 U1 7 U2 16 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 348 EP 363 DI 10.1089/vbz.2014.1703 PG 16 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200004 PM 26086556 ER PT J AU Wilson, WC Daniels, P Ostlund, EN Johnson, DE Oberst, RD Hairgrove, TB Mediger, J McIntosh, MT AF Wilson, William C. Daniels, Peter Ostlund, Eileen N. Johnson, Donna E. Oberst, Richard D. Hairgrove, Thomas B. Mediger, Jessica McIntosh, Michael T. TI Diagnostic Tools for Bluetongue and Epizootic Hemorrhagic Disease Viruses Applicable to North American Veterinary Diagnosticians SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Bluetongue virus; Epizootic hemorrhagic virus; Diagnostic tests; Genotyping; Next-generation sequencing; Real-time PCR; Competitive ELISA ID POLYMERASE-CHAIN-REACTION; LINKED-IMMUNOSORBENT-ASSAY; FOOT-AND-MOUTH; MEDIATED ISOTHERMAL AMPLIFICATION; CULICOIDES-VARIIPENNIS DIPTERA; WEST-NILE-VIRUS; TIME RT-PCR; COMPETITIVE ELISA; CLINICAL-SAMPLES; SONORENSIS DIPTERA AB This review provides an overview of current and potential new diagnostic tests for bluetongue (BT) and epizootic hemorrhagic disease (EHD) viruses compiled from international participants of the Orbivirus Gap Analysis Workshop, Diagnostic Group. The emphasis of this review is on diagnostic tools available to North American veterinary diagnosticians. Standard diagnostic tests are readily available for BT/EHD viruses, and there are described tests that are published in the World Organization for Animal Health (OIE) Terrestrial Manual. There is however considerable variation in the diagnostic approach to these viruses. Serological assays are well established, and many laboratories are experienced in running these assays. Numerous nucleic acid amplification assays are also available for BT virus (BTV) and EHD virus (EHDV). Although there is considerable experience with BTV reverse-transcriptase PCR (RT-PCR), there are no standards or comparisons of the protocols used by various state and federal veterinary diagnostic laboratories. Methods for genotyping BTV and EHDV isolates are available and are valuable tools for monitoring and analyzing circulating viruses. These methods include RT-PCR panels or arrays, RT-PCR and sequencing of specific genome segments, or the use of next-generation sequencing. In addition to enabling virus characterization, use of advanced molecular detection methods, including DNA microarrays and next-generation sequencing, significantly enhance the ability to detect unique virus strains that may arise through genetic drift, recombination, or viral genome segment reassortment, as well as incursions of new virus strains from other geographical areas. C1 [Wilson, William C.] ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. [Daniels, Peter] CSIRO Australian Anim Hlth Lab, Geelong, Vic, Australia. [Ostlund, Eileen N.; Johnson, Donna E.] APHIS, Natl Vet Serv Labs, USDA, VS,Sci Technol & Anal Serv, Ames, IA USA. [Oberst, Richard D.] Kansas State Univ, Coll Vet Med, Kansas Vet Diagnost Lab, Manhattan, KS 66506 USA. [Hairgrove, Thomas B.] Texas A&M Univ, College Stn, TX USA. [Mediger, Jessica] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. [McIntosh, Michael T.] APHIS, Foreign Anim Dis Diagnost Lab, USDA, VS,STAS,NVSL,Plum Isl Dis Ctr, Greenport, NY USA. RP Wilson, WC (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. EM willliam.wilson@ars.usda.gov NR 101 TC 1 Z9 1 U1 1 U2 5 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 364 EP 373 DI 10.1089/vbz.2014.1702 PG 10 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200005 PM 26086557 ER PT J AU Pfannenstiel, RS Mullens, BA Ruder, MG Zurek, L Cohnstaedt, LW Nayduch, D AF Pfannenstiel, Robert S. Mullens, Bradley A. Ruder, Mark G. Zurek, Ludek Cohnstaedt, Lee W. Nayduch, Dana TI Management of North American Culicoides Biting Midges: Current Knowledge and Research Needs SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Bluetongue; Epizootic hemorrhagic disease; BTV; EHDV; Insecticides; Vector ID EPIZOOTIC HEMORRHAGIC-DISEASE; WHITE-TAILED DEER; SONORENSIS DIPTERA-CERATOPOGONIDAE; VARIIPENNIS COMPLEX DIPTERA; WASTE-WATER PONDS; NEW-YORK-STATE; BLUETONGUE-VIRUS; UNITED-STATES; IMMUNOMARKING TECHNIQUE; IMMATURE POPULATIONS AB Culicoides biting midges (Diptera: Ceratopogonidae) are biological vectors of two important viruses impacting North American ruminantsbluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV). These viruses have been identified for over 60 years in North America, but we still lack an adequate understanding of the basic biology and ecology of the confirmed vector, Culicoides sonorensis, and know even less about other putative Culicoides vector species. The major gaps in our knowledge of the biology of Culicoides midges are broad and include an understanding of the ecology of juveniles, the identity of potential alternate vector species, interactions of midges with both pathogens and vertebrates, and the effectiveness of potential control measures. Due to these broad and numerous fundamental knowledge gaps, vector biologists and livestock producers are left with few options to respond to or understand outbreaks of EHD or BT in North America, or respond to emerging or exotic Culicoides-transmitted pathogens. Here we outline current knowledge of vector ecology and control tactics for North American Culicoides species, and delineate research recommendations aimed to fill knowledge gaps. C1 [Pfannenstiel, Robert S.; Ruder, Mark G.; Cohnstaedt, Lee W.; Nayduch, Dana] ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS 66502 USA. [Mullens, Bradley A.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Zurek, Ludek] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Pfannenstiel, RS (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, USDA, 1515 Coll Ave, Manhattan, KS 66502 USA. EM bob.pfannenstiel@ars.usda.gov FU USDA Agricultural Research Service (ARS), National Program for Animal Health FX We would like to thank the organizing committee and the USDA Agricultural Research Service (ARS), National Program for Animal Health for sponsoring and conducting a gap analysis workshop for BTV and EHDV during May, 2013, in Manhattan, Kansas, at the Center for Grain and Animal Health Research (CGAHR, USDA-ARS). Additional thanks are extended to Justin Talley (Oklahoma State University) and Dustin Swanson (Thomas Moore College), as well as two anonymous reviewers for their comments and improvement of this manuscript. NR 96 TC 1 Z9 1 U1 5 U2 11 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 374 EP 384 DI 10.1089/vbz.2014.1705 PG 11 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200006 PM 26086558 ER PT J AU McVey, DS MacLachlan, NJ AF McVey, D. Scott MacLachlan, N. James TI Vaccines for Prevention of Bluetongue and Epizootic Hemorrhagic Disease in Livestock: A North American Perspective SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Arbovirus; Orbivirus; Bluetongue; Vaccines; Epizootic hemorrhagic disease; Immunity ID VIRUS SEROTYPE 8; OUTER CAPSID PROTEINS; INACTIVATED VACCINE; UNITED-STATES; NEUTRALIZING EPITOPES; ANTIBODY-RESPONSES; TRANSPLACENTAL TRANSMISSION; MONOCLONAL-ANTIBODIES; MEDITERRANEAN BASIN; PROTECTIVE EFFICACY AB Bluetongue (BT) and epizootic hemorrhagic disease (EHD) are noncontagious, insect-transmitted diseases of domestic and wild ruminants caused by related but distinct viruses. There are significant gaps in our scientific knowledge and available countermeasures to control an outbreak of orbivirus-induced disease, whether BT or EHD. Both BT virus (BTV) and EHD virus (EHDV) cause hemorrhagic fevers in susceptible ruminants; however, BT is principally a disease of domestic livestock whereas EHD is principally a disease of certain species of wild, non-African ungulates, notably white-tailed deer. The live-attenuated (modified live virus [MLV]) vaccines available in the United States for use in small ruminant livestock do provide good protection against clinical disease following infection with the homologous virus serotype. Although there is increasing justification that the use of MLV vaccines should be avoided if possible, these are the only vaccines currently available in the United States. Specifically, MLVs are used in California to protect sheep against infection with BTV serotypes 10, 11, and 17, and a MLV to BTV serotype 10 is licensed for use in sheep throughout the United States. These MLV vaccines may need to continue to be used in the immediate future for protective immunization of sheep and goats against BT. There are currently no licensed vaccines available for EHD in the United States other than autogenous vaccines. If there is a need to rapidly develop a vaccine to meet an emerging crisis associated with either BTV or EHDV infections, development of an inactivated virus vaccine in a conventional adjuvanted formulation will likely be required. With two doses of vaccine (and in some instances just one dose), inactivated vaccines can provide substantial immunity to the epizootic serotype of either BTV or EHDV. This strategy is similar to that used in the 2006-2008 BTV serotype 8 outbreaks in northern Europe that provided vaccine to the field within 2 years of the initial incursion (by 2008). Further research and development are warranted to provide more efficacious and effective vaccines for control of BTV and EHDV infections. C1 [McVey, D. Scott] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [MacLachlan, N. James] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA. RP McVey, DS (reprint author), ARS, USDA, ABADRU, 115 Coll Ave, Manhattan, KS 66502 USA. EM scott.mcvey@ars.usda.gov FU US Department of Agriculture (USDA) Agricultural Research Service (ARS), National Program for Animal Health FX The authors gratefully thank Dr. Cyril Gay and the US Department of Agriculture (USDA) Agricultural Research Service (ARS), National Program for Animal Health for sponsoring and conducting a recent Orbivirus Research symposium (May, 2013) in Manhattan, Kansas, at the USDA ARS Center for Grain and Animal Health Research. The authors were grateful to participate and wish to state their appreciation for all of the participants. This paper will be part of the report of the symposium. The authors also thank the staff of the Arthropod-Borne Animal Diseases Research Unit and the Center for Grain and Animal Health Research in Manhattan for on-site support of the symposium. NR 136 TC 5 Z9 5 U1 1 U2 11 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN 1 PY 2015 VL 15 IS 6 BP 385 EP 396 DI 10.1089/vbz.2014.1698 PG 12 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CU9XU UT WOS:000363901200007 PM 26086559 ER PT J AU Liu, Z Meyers, KJ Johnson, EJ Snodderly, M Tinker, L Wallace, R Sarto, G Mares, JA AF Liu, Zhe Meyers, Kristin J. Johnson, Elizabeth J. Snodderly, Max Tinker, Lesley Wallace, Robert Sarto, Gloria Mares, Julie A. TI Exposure to Lutein in Infancy via Breast Milk and Later Life Macular Pigment Optical Density SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Liu, Zhe; Meyers, Kristin J.; Mares, Julie A.] Univ Wisconsin, Dept Ophthalmol & Visual Sci, Madison, WI USA. [Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Snodderly, Max] Univ Texas Austin, Inst Neurosci, Austin, TX 78712 USA. [Snodderly, Max] Univ Texas Austin, Ctr Perceptual Syst, Austin, TX 78712 USA. [Tinker, Lesley] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Wallace, Robert] Univ Iowa, Dept Epidemiol, Iowa City, IA USA. [Sarto, Gloria] Univ Wisconsin, Dept Obstet & Gynecol, Madison, WI 53706 USA. NR 0 TC 1 Z9 1 U1 0 U2 3 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 192 PG 3 WC Ophthalmology SC Ophthalmology GA CT5WQ UT WOS:000362882200182 ER PT J AU Mares, JA Meyers, KJ Liu, Z Gehrs, K Iyengar, SK Tinker, L Wang, SJ Gangnon, R Bernstein, PS Johnson, EJ AF Mares, Julie A. Meyers, Kristin J. Liu, Zhe Gehrs, Karen Iyengar, Sudha K. Tinker, Lesley Wang, Sijian Gangnon, Ronald Bernstein, Paul S. Johnson, Elizabeth J. TI Joint Phenotypic and Genotypic Predictors of Macular Pigment Optical Density SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Mares, Julie A.; Meyers, Kristin J.; Liu, Zhe] Univ Wisconsin Madison, Dept Ophthalmol & Visual Sci, Madison, WI USA. [Gehrs, Karen] Univ Iowa, Dept Ophthalmol & Visual Sci, Coralville, IA USA. [Iyengar, Sudha K.] Case Western Reserve Univ, Dept Epidemiolgy & Biostat, Cleveland, OH USA. [Tinker, Lesley] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Wang, Sijian; Gangnon, Ronald] Univ Wisconsin Madison, Dept Populat Hlth Sci, Madison, WI USA. [Bernstein, Paul S.] Univ Utah Hlth Care, Moran Eye Ctr, Salt Lake City, UT USA. [Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 1082 PG 3 WC Ophthalmology SC Ophthalmology GA CT5WQ UT WOS:000362882202403 ER PT J AU Meyers, KJ Liu, Z Wang, SJ Klein, ML Wallace, R Tinker, L Blodi, BA Johnson, EJ Iyengar, SK Mares, JA AF Meyers, Kristin J. Liu, Zhe Wang, Sijian Klein, Michael L. Wallace, Robert Tinker, Lesley Blodi, Barbara A. Johnson, Elizabeth J. Iyengar, Sudha K. Mares, Julie A. TI Phenotypic and Genotypic Predictors of Low Retinal Response to Diets High in Lutein SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Meyers, Kristin J.; Liu, Zhe; Blodi, Barbara A.; Mares, Julie A.] Univ Wisconsin, Dept Ophthalmol & Visual Sci, Madison, WI USA. [Wang, Sijian] Univ Wisconsin, Dept Biostat & Med Informat, Madison, WI USA. [Klein, Michael L.] Oregon Hlth & Sci Univ, Casey Eye Inst, Portland, OR 97201 USA. [Wallace, Robert] Univ Iowa, Dept Epidemiolgy, Iowa City, IA USA. [Tinker, Lesley] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Iyengar, Sudha K.] Case Western Reserve Univ, Dept Epidemiol & Biostat, Cleveland, OH 44106 USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 1083 PG 3 WC Ophthalmology SC Ophthalmology GA CT5WQ UT WOS:000362882202404 ER PT J AU Rowan, S Jiang, SH Chang, ML Cassalman, C McGuire, C Baleja, JD Nagel, BA Fliesler, SJ Smith, D Taylor, A AF Rowan, Sheldon Jiang, Shuhong Chang, Min-Lee Cassalman, Christa McGuire, Christina Baleja, James D. Nagel, Barbara A. Fliesler, Steven J. Smith, Donald Taylor, Allen TI Dietary Glycemia Contributes Reversibly to Age-related Macular Degeneration and Metabolic Disease in Aged C57Bl/6J Mice SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract CT Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) CY MAY 03-07, 2015 CL Denver, CO SP Assoc Res Vis & Ophthalmol C1 [Rowan, Sheldon; Jiang, Shuhong; Chang, Min-Lee; Smith, Donald; Taylor, Allen] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Cassalman, Christa] Tufts Med Ctr, Pathol & Lab Med, Boston, MA USA. [McGuire, Christina; Baleja, James D.] Tufts Univ, Sch Med, Dev Mol & Chem Biol, Boston, MA 02111 USA. [Nagel, Barbara A.] St Louis Univ, Pathol, St Louis, MO 63103 USA. [Fliesler, Steven J.] SUNY Buffalo, Biochem & Ophthalmol, Buffalo, NY 14260 USA. [Fliesler, Steven J.] SUNY Eye Inst, Buffalo, NY USA. [Fliesler, Steven J.] Vet Adm Western New York Healthcare Syst, Res Serv, Buffalo, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2015 VL 56 IS 7 MA 2333 PG 3 WC Ophthalmology SC Ophthalmology GA CT5WQ UT WOS:000362882205334 ER PT J AU Fountain, JC Khera, P Yang, LM Nayak, SN Scully, BT Lee, RD Chen, ZY Kemerait, RC Varshney, RK Guo, BZ AF Fountain, Jake C. Khera, Pawan Yang, Liming Nayak, Spurthi N. Scully, Brian T. Lee, Robert D. Chen, Zhi-Yuan Kemerait, Robert C. Varshney, Rajeev K. Guo, Baozhu TI Resistance to Aspergillus flavus in maize and peanut: Molecular biology, breeding, environmental stress, and future perspectives SO CROP JOURNAL LA English DT Article DE Host resistance; Molecular breeding; Aflatoxin contamination; Reactive oxygen species; ROS ID PREHARVEST AFLATOXIN CONTAMINATION; PATHOGENESIS-RELATED PROTEIN; WRKY53 TRANSCRIPTION FACTOR; INDUCED ANTIOXIDANT DEFENSE; DROUGHT TOLERANCE TRAITS; ARACHIS-HYPOGAEA; SECONDARY METABOLISM; MYCOTOXIN PRODUCTION; SOIL-TEMPERATURE; GENE-EXPRESSION AB The colonization of maize (Zea mays L.) and peanut (Arachis hypogaea L.) by the fungal pathogen Aspergillus flavus results in the contamination of kernels with carcinogenic mycotoxins known as aflatoxins leading to economic losses and potential health threats to humans. The regulation of aflatoxin biosynthesis in various Aspergillus spp. has been extensively studied, and has been shown to be related to oxidative stress responses. Given that environmental stresses such as drought and heat stress result in the accumulation of reactive oxygen species (ROS) within host plant tissues, host-derived ROS may play an important role in cross-kingdom communication between host plants and A. flavus. Recent technological advances in plant breeding have provided the tools necessary to study and apply knowledge derived from metabolomic, proteomic, and transcriptomic studies in the context of productive breeding populations. Here, we review the current understanding of the potential roles of environmental stress, ROS, and aflatoxin in the interaction between A. flavus and its host plants, and the current status in molecular breeding and marker discovery for resistance to A. flavus colonization and aflatoxin contamination in maize and peanut. We will also propose future directions and a working model for continuing research efforts linking environmental stress tolerance and aflatoxin contamination resistance in maize and peanut. (C) 2015 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. All rights reserved. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Fountain, Jake C.; Khera, Pawan; Yang, Liming; Kemerait, Robert C.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Khera, Pawan; Nayak, Spurthi N.; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Telangana, India. [Khera, Pawan; Yang, Liming; Guo, Baozhu] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Yang, Liming] Huaiyin Normal Univ, Sch Life Sci, Huaian, Jiangsu, Peoples R China. [Scully, Brian T.] ARS, USDA, US Hort Lab, Ft Pierce, FL USA. [Lee, Robert D.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA USA. [Chen, Zhi-Yuan] Louisiana State Univ, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. RP Guo, BZ (reprint author), ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. EM baozhu.guo@ars.usda.gov NR 89 TC 4 Z9 4 U1 10 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD JUN PY 2015 VL 3 IS 3 SI SI BP 229 EP 237 DI 10.1016/j.cj.2015.02.003 PG 9 WC Agronomy SC Agriculture GA CT2YJ UT WOS:000362672200007 ER PT J AU Haider, N Rahman, MS Khan, SU Mikolon, A Osmani, MG Gurley, ES Shanta, IS Paul, SK Macfarlane-Berry, L Islam, A Islam, A Desmond, J Epstein, JH Priestley, RA Kersh, GJ Rahman, MZ Daszak, P Luby, SP Massung, RF Zeidner, N AF Haider, Najmul Rahman, Md. Shafiqur Khan, Salah Uddin Mikolon, Andrea Osmani, Muzaffor G. Gurley, Emily S. Shanta, Ireen Sultana Paul, Suman Kumer Macfarlane-Berry, Laura Islam, Ariful Islam, Ausraful Desmond, James Epstein, Jonathan H. Priestley, Rachael A. Kersh, Gilbert J. Rahman, Mohammed Ziaur Daszak, Peter Luby, Stephen P. Massung, Robert F. Zeidner, Nord TI Serological Evidence of Coxiella burnetii Infection in Cattle and Goats in Bangladesh SO ECOHEALTH LA English DT Article DE seroprevalence; C. burnetii; Q fever; cattle; goat; Bangladesh ID Q-FEVER; PREVALENCE; ANTIBODIES; ANIMALS AB We tested 1149 ruminant sera conveniently collected from three districts of Bangladesh to identify the serological evidence of Coxiella burnetii infection in cattle and goats by enzyme-linked immunosorbent assay. We found that 0.7% (8/1149) of ruminants had detectable immunoglobulin G for C. burnetii: 0.65% (4/620) in cattle and 0.76% (4/529) in goats. A sub-set of ruminant samples was retested and confirmed by immunofluorescence assay (18/112). Although we cannot rule out false-positive reactions, our study suggests the presence of C. burnetii in cattle and goats in Bangladesh. Further studies are required to estimate disease burden at the population level and identify risk factors for Q fever in ruminants in Bangladesh. C1 [Haider, Najmul; Rahman, Md. Shafiqur; Khan, Salah Uddin; Gurley, Emily S.; Shanta, Ireen Sultana; Paul, Suman Kumer; Macfarlane-Berry, Laura; Islam, Ausraful; Rahman, Mohammed Ziaur; Luby, Stephen P.; Zeidner, Nord] Int Ctr Diarrheal Dis Res Bangladesh Icddr B, Ctr Communicable Dis, Zoonot Dis Res Grp, Dhaka, Bangladesh. [Mikolon, Andrea] USDA, Hawthorne, CA USA. [Osmani, Muzaffor G.] Minist Fisheries & Livestock, Dept Livestock Serv, Dhaka, Bangladesh. [Islam, Ariful; Desmond, James; Epstein, Jonathan H.; Daszak, Peter] EcoHlth Alliance, New York, NY USA. [Priestley, Rachael A.; Kersh, Gilbert J.; Massung, Robert F.; Zeidner, Nord] Ctr Dis Control & Prevent, Atlanta, GA USA. [Luby, Stephen P.] Stanford Univ, Woods Inst Environm, Stanford, CA 94305 USA. RP Haider, N (reprint author), Int Ctr Diarrheal Dis Res Bangladesh Icddr B, Ctr Communicable Dis, Zoonot Dis Res Grp, Dhaka, Bangladesh. EM nazmulh@icddrb.org RI Gurley, Emily/B-7903-2010; OI Gurley, Emily/0000-0002-8648-9403; Luby, Stephen/0000-0001-5385-899X; Haider, Najmul/0000-0002-5980-3460 FU Google; Rockefeller foundation through EcoHealth Alliance FX This research was funded by Google and the Rockefeller foundation through EcoHealth Alliance. icddr,b acknowledges with gratitude the commitment of Google and the Rockefeller foundation to its research efforts. We acknowledge the veterinarians of Department of Livestock Services of Bangladesh as well as Chittagong Veterinary and Animal Sciences University who assisted with various aspects of this project. We are grateful to Ms. Diana DiazGranados, icddr,b, for her constructive support in editing this manuscript. NR 19 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD JUN PY 2015 VL 12 IS 2 BP 354 EP 358 DI 10.1007/s10393-015-1011-x PG 5 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CS0JZ UT WOS:000361746600020 PM 25649716 ER PT J AU Ballash, GA Dubey, JP Kwok, OCH Shoben, AB Robison, TL Kraft, TJ Dennis, PM AF Ballash, Gregory A. Dubey, J. P. Kwok, O. C. H. Shoben, Abigail B. Robison, Terry L. Kraft, Tom J. Dennis, Patricia M. TI Seroprevalence of Toxoplasma gondii in White-Tailed Deer (Odocoileus virginianus) and Free-Roaming Cats (Felis catus) Across a Suburban to Urban Gradient in Northeastern Ohio SO ECOHEALTH LA English DT Article DE seroprevalence; Toxoplasma gondii; white-tailed deer; free-roaming cats; urbanization ID UNITED-STATES; NEOSPORA-CANINUM; DOMESTIC CATS; GENETIC-CHARACTERIZATION; RISK-FACTORS; HOME-RANGE; ANTIBODIES; PREVALENCE; INFECTION; TRANSMISSION AB Felids serve as the definitive host of Toxoplasma gondii contaminating environments with oocysts. White-tailed deer (WTD; Odocoileus virginianus) are used as sentinel species for contaminated environments as well as a potential source for human foodborne infection with T. gondii. Here we determine the seroprevalence of T. gondii in a WTD and felid population, and examine those risk factors that increase exposure to the parasite. Serum samples from 444 WTD and 200 free-roaming cats (Felis catus) from urban and suburban reservations were tested for T. gondii antibodies using the modified agglutination test (MAT, cut-off 1:25). Antibodies to T. gondii were found in 261 (58.8%) of 444 WTD, with 164 (66.1%) of 248 from urban and 97 (49.5%) of 196 from suburban regions. Significant risk factors for seroprevalence included increasing age (P < 0.0001), reservation type (P < 0.0001), and household densities within reservation (P < 0.0001). Antibodies to T. gondii were found in 103 (51.5%) of 200 cats, with seroprevalences of 79 (51%) of 155 and 24 (53.3%) of 45 from areas surrounding urban and suburban reservations, respectively. Seroprevalence did not differ by age, gender, or reservation among the cats' sample. Results indicate WTD are exposed by horizontal transmission, and this occurs more frequently in urban environments. The difference between urban and suburban cat densities is the most likely the reason for an increased seroprevalence in urban WTD. These data have public health implications for individuals living near or visiting urban areas where outdoor cats are abundant as well as those individuals who may consume WTD venison. C1 [Ballash, Gregory A.; Dennis, Patricia M.] Ohio State Univ, Dept Vet Prevent Med, Columbus, OH 43210 USA. [Dubey, J. P.; Kwok, O. C. H.] USDA ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Shoben, Abigail B.] Ohio State Univ, Coll Publ Hlth, Div Biostat, Columbus, OH 43210 USA. [Robison, Terry L.; Kraft, Tom J.] Cleveland Metropk, Dept Planning Design & Nat Resources, Fairview Pk, OH 44126 USA. [Dennis, Patricia M.] Cleveland Metropk Zoo, Sarah Allison Steffee Ctr Zool Med, Cleveland, OH 44109 USA. RP Dennis, PM (reprint author), Ohio State Univ, Dept Vet Prevent Med, 1920 Coffey Rd, Columbus, OH 43210 USA. EM pmd@clevelandmetroparks.com RI Dennis, Patricia/A-2081-2015 FU Cleveland Metroparks; Cleveland Metroparks Zoo FX We would like to thank the Cleveland Metroparks staff, Dr. Thomas Wittum and Dr. Nidia Arguedas for helping with sample collection, statistical coding, and figure design. The project was funded by the Cleveland Metroparks and the Cleveland Metroparks Zoo. NR 36 TC 2 Z9 2 U1 8 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD JUN PY 2015 VL 12 IS 2 BP 359 EP 367 DI 10.1007/s10393-014-0975-2 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CS0JZ UT WOS:000361746600021 PM 25269422 ER PT J AU Brito, A Mujica-Coopman, MF Olivares, M de Romana, DL Cori, H Allen, LH AF Brito, Alex Mujica-Coopman, Maria F. Olivares, Manuel Lopez de Romana, Daniel Cori, Hector Allen, Lindsay H. TI Folate and Vitamin B-12 Status in Latin America and the Caribbean: An Update SO FOOD AND NUTRITION BULLETIN LA English DT Article DE folate; folic acid; Latin America; prevalence; vitamin; vitamin B-12 ID NEURAL-TUBE DEFECTS; FOLIC-ACID FORTIFICATION; NUTRITIONAL-STATUS; SERUM HOMOCYSTEINE; COSTA-RICA; MICRONUTRIENT DEFICIENCIES; COBALAMIN DEFICIENCY; REPRODUCTIVE AGE; GUATEMALAN WOMEN; IRON-DEFICIENCY AB Background: The current magnitude of folate and vitamin B-12 deficiency in Latin America and the Caribbean is uncertain. Objective: To summarize data on plasma or serum vitamin B-12 and folate concentrations in Latin America and the Caribbean reported since 1990, a period that covers the era before and after the introduction of folic acid fortification. Methods: A systematic review was conducted in 2012 and updated in 2014. Studies and surveys using biochemical biomarkers and conducted in apparently healthy individuals were identified. Results: Folate deficiency in Latin America and the Caribbean appears not to be a public health problem (prevalence < 5%) after the introduction of folic acid fortification. However, there is some indication that high rates of low or marginal vitamin B-12 status remain in most locations and across population groups. Conclusions: Adding vitamin B-12 as a fortificant with folic acid may be the best strategy in areas where vitamin B-12 deficiency is an established concern. C1 [Brito, Alex; Allen, Lindsay H.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Mujica-Coopman, Maria F.; Olivares, Manuel] Univ Chile, Inst Nutr & Food Technol INTA, Santiago, Chile. [Lopez de Romana, Daniel] Micronutrient Initiat, Ottawa, ON, Canada. [Cori, Hector] DSM Nutrit Prod, Santiago, Chile. RP Allen, LH (reprint author), Univ Calif Davis, ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM abrito@ucdavis.edu; Lindsay.Allen@ars.usda.gov FU DSM Nutritional Products FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: DSM Nutritional Products provided funding for this study. NR 68 TC 2 Z9 2 U1 2 U2 10 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2015 VL 36 SU 2 BP S109 EP S118 DI 10.1177/0379572115585772 PG 10 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CR2UC UT WOS:000361185100003 PM 26125196 ER PT J AU Cediel, G Olivares, M Brito, A Cori, H de Romana, DL AF Cediel, Gustavo Olivares, Manuel Brito, Alex Cori, Hector Lopez de Romana, Daniel TI Zinc Deficiency in Latin America and the Caribbean SO FOOD AND NUTRITION BULLETIN LA English DT Article DE zinc; deficiency; stunting; Latin America; Caribbean ID RANDOMIZED CONTROLLED-TRIALS; DIETARY ZINC; DEVELOPING-COUNTRIES; MEXICAN CHILDREN; NUTRITION SURVEY; POPULATION; IRON; INDICATORS; PROGRESS; COPPER AB Background: Zinc deficiency affects multiple vital functions in the life cycle, especially growth. Limited information is available on the magnitude of zinc deficiency in Latin America and the Caribbean. Objective: To examine the latest available information on both the prevalence of zinc deficiency and the risk of zinc deficiency in Latin America and the Caribbean. Methods: The prevalence of zinc deficiency was identified through a systematic review looking for the latest available data on serum zinc concentrations from surveys or studies with national representativeness conducted in Latin America and the Caribbean. The risk of zinc deficiency in Latin America and the Caribbean was estimated based on dietary zinc inadequacy (according to the 2011 National Food Balance Sheets) and stunting in children under 5 years of age. Results: Only four countries had available national biochemical data. Mexican, Colombian, Ecuadorian, and Guatemalan children under 6 years of age and women 12 to 49 years of age had a high prevalence of zinc deficiency (19.1% to 56.3%). The countries with the highest risk of zinc deficiency (estimated prevalence of inadequate zinc intake >25% plus prevalence of stunting >20%) were Belize, Bolivia, El Salvador, Guatemala, Haiti, Honduras, Nicaragua, and Saint Vincent and the Grenadines. Zinc dietary inadequacy was directly correlated with stunting (r = 0.64, p < .001). Conclusions: Prevalence data from the four available Latin America and Caribbean national surveys indicate a high prevalence of zinc deficiency in children under 6 years of age and women 12 to 49 years of age. High rates of both estimated zinc dietary inadequacy and stunting were also reported in most Latin America and Caribbean countries. C1 [Cediel, Gustavo; Olivares, Manuel] Univ Chile, Micronutrients Lab, Inst Nutr & Food Technol, Santiago, Chile. [Brito, Alex] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Cori, Hector] Nutr Sci Director Latin Amer DSM Nutrit Prod, Ottawa, ON, Canada. [Lopez de Romana, Daniel] Micronutrient Initiat, Ottawa, ON, Canada. RP de Romana, DL (reprint author), Jiron Batallon Libres Trujillo 159, Apt 102,Santiago Surco, Lima, Peru. EM dlopezderomana@gmail.com FU DSM Nutritional Products FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study received financial support for the research and publication by DSM Nutritional Products. NR 37 TC 2 Z9 3 U1 1 U2 9 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2015 VL 36 SU 2 BP S129 EP S138 DI 10.1177/0379572115585781 PG 10 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CR2UC UT WOS:000361185100005 PM 26125198 ER PT J AU Cediel, G Olivares, M Brito, A de Roman, DL Cori, H La Frano, MR AF Cediel, Gustavo Olivares, Manuel Brito, Alex de Roman, Daniel Lopez Cori, Hector La Frano, Michael R. TI Interpretation of Serum Retinol Data From Latin America and the Caribbean SO FOOD AND NUTRITION BULLETIN LA English DT Article DE Vitamin A; retinol; deficiency; Latin America; Caribbean ID VITAMIN-A-DEFICIENCY; CHILDREN; FORTIFICATION; PROGRAM; SUGAR; IRON; XEROPHTHALMIA; METAANALYSIS; GUATEMALA; LIVER AB Background: In recent decades, the general socioeconomic situation in Latin America and the Caribbean countries has improved, and many vitamin A programs have been implemented in an attempt to reduce vitamin A deficiency in the region. Objective: To examine vitamin A status in Latin America and the Caribbean based on serum retinol concentrations and to contrast available data published before and after 1998. Methods: A systematic review was performed. National surveys or representative studies that reported vitamin A status were selected. Results: Ten national surveys and six representative studies were identified. Data for children under 6 years of age indicate that Guatemala and Nicaragua have practically eradicated vitamin A deficiency (less than 2% prevalence of serum retinol < 20 mu g/dL). In Costa Rica, Cuba, El Salvador, and Panama, the prevalence of vitamin A deficiency ranged from 2.8% to 9.4%. In Peru, Honduras, Argentina, Ecuador, and Brazil, vitamin A deficiency is a moderate public health problem (prevalence from 14.0% to 17.4%), while in Colombia, Mexico, and Haiti it is a severe public health problem (prevalence from 24.3% to 32.0%). Disadvantaged groups (indigenous people and those of Afro-Colombian descent) have the highest rates of deficiency. The prevalence of vitamin A deficiency is under 20% in schoolchildren and adult women. When data published before and after 1998 for children under 6 years of age were compared, most Central American countries had a reduction in the prevalence of vitamin A deficiency (p < .05), whereas in South American countries, the prevalence of vitamin A deficiency increased over time (p < .05). Conclusions: The prevalence of vitamin A deficiency in children under 6 years of age has decreased in many Central American countries, but vitamin A deficiency still remains a public health problem in numerous Latin America and Caribbean countries, especially among disadvantaged and vulnerable groups. Because of issues with the accuracy of the serum retinol biomarker reflecting body stores, these results must be interpreted with caution. C1 [Cediel, Gustavo; Olivares, Manuel] Univ Chile, Micronutrients Lab, Inst Nutr & Food Technol, Santiago 7830490, Chile. [Brito, Alex; La Frano, Michael R.] Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [de Roman, Daniel Lopez] Micronutrient Initiat, Ottawa, ON, Canada. [Cori, Hector] DSM Nutrit Prod, Santiago, Chile. RP Cediel, G (reprint author), Univ Chile, Inst Nutr & Food Technol, El Libano 5524, Santiago 7830490, Chile. EM gcediel@inta.uchile.cl OI La Frano, Michael/0000-0002-2269-1688 FU DSM Nutritional Products FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study received financial support for the research and publication by DSM Nutritional Products. NR 46 TC 3 Z9 3 U1 2 U2 7 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2015 VL 36 SU 2 BP S98 EP S108 DI 10.1177/0379572115585743 PG 11 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CR2UC UT WOS:000361185100002 PM 26125200 ER PT J AU de Romana, DL Olivares, M Brito, A AF Lopez de Romana, Daniel Olivares, Manuel Brito, Alex TI Introduction: Prevalence of Micronutrient Deficiencies in Latin America and the Caribbean SO FOOD AND NUTRITION BULLETIN LA English DT Editorial Material DE anemia; folate; folic acid; Latin America; micronutrients; minerals; prevalence; vitamin A; vitamin B-12; vitamins; zinc AB This Food and Nutrition Bulletin supplement summarizes updated prevalence data on micronutrient deficiencies in Latin America and the Caribbean ( LAC). In order to provide an updated view of micronutrient status in LAC, systematic reviews were performed utilizing national health surveys and research-oriented studies focused on the prevalence of deficiencies of vitamin A, folate, anemia ( as a proxy of iron deficiency), and zinc. Results show that the prevalence of vitamin A deficiency has been reduced in many countries, folate deficiency is now almost non-existent, low or marginal vitamin B-12 status is still prevalent in most locations, anemia remains a public health problem among children under 6 years of age and women of childbearing age in most surveyed countries, and there is a high prevalence of zinc deficiency in children under 6 years of age and girls and women 12 to 49 years of age. Thus, regardless of improvements in the overall rates of economic growth in LAC, deficiencies of these micronutrients still remain a public health problem. C1 [Lopez de Romana, Daniel] Micronutrient Initiat, Ottawa, ON, Canada. [Olivares, Manuel] Univ Chile, Inst Nutr & Food Technol INTA, Santiago, Chile. [Brito, Alex] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. RP de Romana, DL (reprint author), Jiron Batallon Libres Trujillo 159, Santiago Surco, Lima, Peru. EM dlopezderomana@gmail.com; abrito@ucdavis.edu NR 6 TC 1 Z9 1 U1 2 U2 8 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2015 VL 36 SU 2 BP S95 EP S97 DI 10.1177/0379572115585736 PG 3 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CR2UC UT WOS:000361185100001 ER PT J AU Mujica-Coopman, MF Brito, A de Romana, DL Rios-Castillo, I Cori, H Olivares, M AF Mujica-Coopman, Maria F. Brito, Alex Lopez de Romana, Daniel Rios-Castillo, Israel Cori, Hector Olivares, Manuel TI Prevalence of Anemia in Latin America and the Caribbean SO FOOD AND NUTRITION BULLETIN LA English DT Article DE anemia; Caribbean; children; iron; Latin America; women ID IRON-DEFICIENCY ANEMIA; YOUNG-CHILDREN; FOLIC-ACID; PROGRAM; HEALTH; WOMEN; AGE; HEPCIDIN; IMPACT; CHILE AB Background: In Latin America and the Caribbean, anemia has been a public health problem that affects mainly women of childbearing age and children under 6 years of age. However, the current prevalence of anemia in this region is unknown. Objective: To examine the latest available prevalence data on anemia in Latin America and the Caribbean. Methods: A systematic review was conducted in 2011 and updated in 2014. Studies determining the prevalence of anemia conducted in apparently healthy populations with national or regional representativeness were included in the review. Results: The lowest prevalence rates of anemia among children under 6 years of age were found in Chile (4.0%), Costa Rica (4.0%), Argentina (7.6%), and Mexico (19.9%). In Nicaragua, Brazil, Ecuador, El Panama, and Honduras, anemia was a moderate public health problem, with prevalence ranging Salvador, Cuba, Colombia, the Dominican Republic, Peru, from 20.1% to 37.3%. Anemia was a severe public health problem in Guatemala, Haiti, and Bolivia. The prevalence of anemia among women of childbearing age was lowest in Chile (5.1%). In Colombia, El Salvador, Costa Rica, Nicaragua, Ecuador, Mexico, Peru, Honduras, and Argentina, anemia was a mild public health problem, with prevalence ranging from 7.6% to 18.7%. In Guatemala, Brazil, the Dominican Republic, and Bolivia, anemia was a moderate public health problem, with prevalence ranging from 21.4% to 38.3%. Panama and Haiti had the highest reported prevalence rates (40.0% and 45.5%, respectively), and anemia was considered a severe public health problem in those countries. Conclusions: Anemia remains a public health problem in children under 6 years of age and women of childbearing age in most Latin America and Caribbean countries for which data are available. C1 [Mujica-Coopman, Maria F.; Olivares, Manuel] Inst Nutr & Food Technol INTA, Micronutrient Lab, Santiago, Chile. [Brito, Alex] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Lopez de Romana, Daniel] Micronutrient Initiat, Ottawa, ON, Canada. [Rios-Castillo, Israel] Nutrit Res & Comprehens Dev Fdn, Panama City, Panama. [Cori, Hector] DSM Nutrit Prod, Santiago, Chile. RP Olivares, M (reprint author), Univ Chile, INTA, Micronutrient Lab, El Libano 5524, Santiago 832000, Chile. EM molivare@inta.uchile.cl FU DSM Nutritional Products FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: DSM Nutritional Products provided funding for this study. NR 58 TC 6 Z9 7 U1 4 U2 10 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2015 VL 36 SU 2 BP S119 EP S128 DI 10.1177/0379572115585775 PG 10 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CR2UC UT WOS:000361185100004 PM 26125197 ER PT J AU Tryon, MS Stanhope, KL Epel, ES Mason, AE Brown, R Medici, V Havel, PJ Laugero, KD AF Tryon, Matthew S. Stanhope, Kimber L. Epel, Elissa S. Mason, Ashley E. Brown, Rashida Medici, Valentina Havel, Peter J. Laugero, Kevin D. TI Excessive Sugar Consumption May Be a Difficult Habit to Break: A View From the Brain and Body SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID CORTICOTROPIN-RELEASING-FACTOR; CHRONIC STRESS; COMFORT FOOD; ADRENALECTOMIZED RATS; NEURAL CIRCUITS; AXIS; RESPONSES; CORTISOL; OBESITY; SYSTEM AB Context: Sugar overconsumption and chronic stress are growing health concerns because they both may increase the risk for obesity and its related diseases. Rodent studies suggest that sugar consumption may activate a glucocorticoid-metabolic-brain-negative feedback pathway, which may turn off the stress response and thereby reinforce habitual sugar overconsumption. Objective: The objective of the study was to test our hypothesized glucocorticoid-metabolic-brain model in women consuming beverages sweetened with either aspartame of sucrose. Design: This was a parallel-arm, double-masked diet intervention study. Setting: The study was conducted at the University of California, Davis, Clinical and Translational Science Center's Clinical Research Center and the University of California, Davis, Medical Center Imaging Research Center. Participants: Nineteen women (age range 18-40 y) with a body mass index (range 20-34 kg/m(2)) who were a subgroup from a National Institutes of Health-funded investigation of 188 participants assigned to eight experimental groups. Intervention: The intervention consisted of sucrose-or aspartame-sweetened beverage consumption three times per day for 2 weeks. Main Outcome Measures: Salivary cortisol and regional brain responses to the Montreal Imaging Stress Task were measured. Results: Compared with aspartame, sucrose consumption was associated with significantly higher activity in the left hippocampus (P = .001). Sucrose, but not aspartame, consumption associated with reduced (P = .024) stress-induced cortisol. The sucrose group also had a lower reactivity to naltrexone, significantly (P = .041) lower nausea, and a trend (P = .080) toward lower cortisol. Conclusion: These experimental findings support a metabolic-brain-negative feedback pathway that is affected by sugar and may make some people under stress more hooked on sugar and possibly more vulnerable to obesity and its related conditions. C1 [Tryon, Matthew S.; Stanhope, Kimber L.; Havel, Peter J.; Laugero, Kevin D.] Univ Calif Davis, Sch Vet Med, Dept Nutr, Davis, CA 95616 USA. [Havel, Peter J.] Univ Calif Davis, Sch Vet Med, Dept Mol Biosci, Davis, CA 95616 USA. [Medici, Valentina] Univ Calif Davis, Sch Med, Div Gastroenterol & Hepatol, Davis, CA 95616 USA. [Laugero, Kevin D.] ARS, Stress Biol & Nutr Res Lab, Obes & Metab Res Unit, Western Human Nutr Res Ctr,USDA, Davis, CA 95616 USA. [Epel, Elissa S.; Mason, Ashley E.; Brown, Rashida] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA. RP Laugero, KD (reprint author), ARS, Stress Biol & Nutr Res Lab, Obes & Metab Res Unit, Western Human Nutr Res Ctr,USDA, Davis, CA 95616 USA. EM kevin.laugero@ars.usda.gov FU University of California, Office of the President [142691]; US Department of Agriculture's Agricultural Research Service [5306-51530-019-00]; National Institutes of Health (NIH)/National Heart, Lung, and Blood Institute [1R01 HL09133, 1R01 HL107256]; National Center for Research Resources, a component of the NIH; NIH Roadmap for Medical Research [UL1 RR024146]; Building Interdisciplinary Research Careers in Women's Health award [K12 HD051958]; Eunice Kennedy Shriver National Institute of Child Health and Human Development, Office of Research on Women's Health, Office of Dietary Supplements; National Institute of Aging FX This work was supported by a multicampus grant from the University of California, Office of the President (Award 142691; principal investigator E.S.E.) and US Department of Agriculture's Agricultural Research Service intramural Project Number 5306-51530-019-00 (principal investigator K.D.L.). The parent study was supported by the National Institutes of Health (NIH)/National Heart, Lung, and Blood Institute Grant 1R01 HL09133 (principal investigator P.J.H.) and Grant 1R01 HL107256 (principal investigator P.J.H.); National Center for Research Resources, a component of the NIH, and NIH Roadmap for Medical Research Grant UL1 RR024146 (principal investigator L. Berglund). K.L.S. is supported by a Building Interdisciplinary Research Careers in Women's Health award (Grant K12 HD051958; principal investigator E. Gold) supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development, Office of Research on Women's Health, Office of Dietary Supplements, and the National Institute of Aging. NR 37 TC 9 Z9 9 U1 5 U2 25 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD JUN PY 2015 VL 100 IS 6 BP 2239 EP 2247 DI 10.1210/jc.2014-4353 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CQ8ET UT WOS:000360840000035 PM 25879513 ER PT J AU Han, DJ Wang, QL Chen, XM Zeng, QD Wu, JH Xue, WB Zhan, GM Huang, LL Kang, ZS AF Han, D. J. Wang, Q. L. Chen, X. M. Zeng, Q. D. Wu, J. H. Xue, W. B. Zhan, G. M. Huang, L. L. Kang, Z. S. TI Emerging Yr26-Virulent Races of Puccinia striiformis f. tritici Are Threatening Wheat Production in the Sichuan Basin, China SO PLANT DISEASE LA English DT Article ID STRIPE RUST RESISTANCE; ADULT-PLANT RESISTANCE; HIGH-TEMPERATURE; VIRULENCE CHARACTERIZATION; SEEDLING RESISTANCE; POWDERY MILDEW; UNITED-STATES; GENE YR26; CULTIVARS; EPIDEMIOLOGY AB Stripe rust, caused by Puccinia striiformis f. tritici, is one of the most destructive diseases of wheat in the world. The Sichuan Basin is one of the most important regions of wheat production and stripe rust epidemics in China. Stripe rust resistance gene Yr26 (the same gene as Yr24) has been widely used in wheat breeding programs and in many cultivars grown in this region since the gene was discovered in the early 1990s. Virulence to Yr26 has increased in frequency since its first detection in 2008. The objective of this study was to assess the vulnerability of the wheat cultivars and breeding lines in the Sichuan Basin to Yr26-virulent races. In total, 85 wheat accessions were tested with Yr26-avirulent races CYR32, CYR33, and Su11-4 and two Yr26-virulent races, V26/CM42 and V26/Gui22. DNA markers for Yr26 were used to determine the presence and absence of Yr26 in the wheat accessions. Of the 85 wheat accessions, only 5 were resistant and 19 susceptible to all races tested, and the remaining 61 were resistant to at least one or more races tested in seedling stage. In all, 65 (76.5%) accessions were susceptible to the emerging Yr26-virulent race V26/Gui22. In field tests, susceptible accessions increased from 31.8% in a nursery inoculated with predominant and Yr26-avirulent races to 61.2% in the nursery inoculated with the predominant races mixed with V26/Gui22. Based on the results of the molecular marker and race tests, 33 (38.8%) accessions were determined to have Yr26, showing that the Yr26 virulence is a major threat to wheat production in the Sichuan Basin and potentially in other regions of China. C1 [Han, D. J.; Wang, Q. L.; Zeng, Q. D.; Wu, J. H.; Xue, W. B.; Zhan, G. M.; Huang, L. L.; Kang, Z. S.] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. [Han, D. J.] Northwest A&F Univ, Coll Agron, Yangling, Shaanxi, Peoples R China. [Wang, Q. L.; Zhan, G. M.; Huang, L. L.; Kang, Z. S.] Northwest A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China. [Chen, X. M.] Washington State Univ, Wheat Genet Qual Physiol & Dis Res Unit, USDA ARS, Pullman, WA 99164 USA. [Chen, X. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Kang, ZS (reprint author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. EM huanglili@nwsuaf.edu.cn; kangzs@nwsuaf.edu.cn FU National Basic Research Program of China [2013CB127700]; National High-Tech R&D Program of China [2012AA101503]; earmarked fund for Modern Agro-industry Technology Research System [CARS-3-1-11]; National Natural Science Foundation of China [31371924]; Ministry of Education of China [B07049] FX This study was financially supported by the National Basic Research Program of China (number 2013CB127700), the National High-Tech R&D Program of China (number 2012AA101503), the earmarked fund for Modern Agro-industry Technology Research System (number CARS-3-1-11), the National Natural Science Foundation of China (31371924), and the 111 Project from the Ministry of Education of China (B07049). NR 40 TC 10 Z9 13 U1 1 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 754 EP 760 DI 10.1094/PDIS-08-14-0865-RE PG 7 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000002 ER PT J AU Copes, WE AF Copes, Warren E. TI Spread Potential of Binucleate Rhizoctonia from Nursery Propagation Floors to Trays Containing Azalea Stem Cuttings and Sanitary Control Options SO PLANT DISEASE LA English DT Article ID CONTAINER-GROWN AZALEA; WEB BLIGHT DEVELOPMENT; ORNAMENTAL PLANTS; SOLANI AB Binucleate Rhizoctonia sp. (BNR), the cause of web blight, can be spread on azalea stem cuttings into propagation houses, but also can be eliminated from stems by submerging cuttings in 50 degrees C water for 21 min. The overall objective was to evaluate risk of stem cuttings in trays becoming contaminated from inoculum on polypropylene fabric and gravel floors of propagation houses by evaluating four spread aspects in separate experiments. In experiment one, BNR was recovered from 1 to 50% samples from floor areas. In experiment two, BNR survival was higher under 70% shade than full sun, with the presence of organic media than its absence, and on gravel than on fabric. In experiment three in both years, BNR was never recovered from peat in trays beside or on colonized floor materials. In experiment four, disinfestants greatly reduced BNR on ground fabric and significantly on gravel. Results show BNR persists in propagation houses, yet spread into propagation trays is very low. Results imply the importance of cleaning floor surfaces of organic matter. Disinfestants further reduce propagule survival, but may not completely eliminate BNR, especially on gravel. C1 USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. RP Copes, WE (reprint author), USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. EM warren.copes@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service, Thad Cochran Southern Horticultural Laboratory, Southern Horticultural Research Unit [6404-21430-001-00D] FX I thank Debbie Boykin, Midsouth Area ARS Statistician, for statistical support in analyzing sanitation treatments. The research was supported through the United States Department of Agriculture, Agricultural Research Service, Thad Cochran Southern Horticultural Laboratory, Southern Horticultural Research Unit project number 6404-21430-001-00D. NR 19 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 842 EP 847 DI 10.1094/PDIS-09-14-0915-RE PG 6 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000013 ER PT J AU Gent, DH Twomey, MC Wolfenbarger, SN Woods, JL AF Gent, David H. Twomey, Megan C. Wolfenbarger, Sierra N. Woods, Joanna L. TI Pre- and Postinfection Activity of Fungicides in Control of Hop Downy Mildew SO PLANT DISEASE LA English DT Article ID PSEUDOPERONOSPORA-HUMULI; PLASMOPARA-VITICOLA; PERENNIAL RYEGRASS; PHYSICAL-MODES; STEM RUST; MANAGEMENT; INFECTION; ACID; AZOXYSTROBIN; PERSISTENCE AB Optimum timing and use of fungicides for disease control are improved by an understanding of the characteristics of fungicide physical mode of action. Greenhouse and field experiments were conducted to quantify and model the duration of pre- and postinfection activity of fungicides most commonly used for control of hop downy mildew (caused by Pseudoperonospora humuli). In greenhouse experiments, control of downy mildew on leaves was similar among fungicides tested when applied preventatively but varied depending on both the fungicide and the timing of application postinfection. Disease control decreased as applications of copper were made later after inoculation. In contrast, cymoxanil, trifloxystrobin, and dimethomorph reduced disease with similar efficacy when applied 48 h after inoculation compared with preventative applications of these fungicides. When fungicides were applied 72 h after inoculation, only dimethomorph reduced the sporulating leaf area similarly to preinoculation application timing. Adaxial chlorosis, necrosis, and water soaking of inoculated leaves, indicative of infection by P. humuli, were more severe when plants were treated with cymoxanil, trifloxystrobin, and dimethomorph 48 to 72 h after inoculation, even though sporulation was suppressed. Trifloxystrobin and dimethomorph applied 72 h after inoculation suppressed formation of sporangia on sporangiophores as compared with all other treatments. In field studies, dimethomorph, fosetyl-Al, and trifloxystrobin suppressed development of shoots with systemic downy mildew to the greatest extent when applied near the timing of inoculation, although the duration of preventative and postinfection activity varied among the fungicides. There was a small reduction in efficacy of disease control when fosetyl-Al was applied 6 to 7 days after inoculation as compared with protective applications. Trifloxystrobin had 4 to 5 days of pre-infection activity and limited postinfection activity. Dimethomorph had the longest duration of protective activity. Percent disease control was reduced progressively with increasing time between inoculation and application of dimethomorph. These findings provide guidance to the use of fungicides when applications are timed with forecasted or post hoc disease hazard warnings, as well as guidance on tank-mixes of fungicides that may be suitable both for resistance management considerations and extending intervals between applications. C1 [Gent, David H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, David H.; Twomey, Megan C.; Wolfenbarger, Sierra N.; Woods, Joanna L.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Gent, DH (reprint author), Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU Oregon State University Agricultural Research Foundation; Oregon Hop Commission; USDA-ARS CRIS [5358-21000-035-00] FX Funding for these studies was provided by the Oregon State University Agricultural Research Foundation, Oregon Hop Commission, and USDA-ARS CRIS Project 5358-21000-035-00. We thank D. Johnson and C. Ocamb for providing helpful suggestions that improved the manuscript; and S. Massie, Capital Farms, and F and B Nursery for providing the time, resources, and facilities that made this work possible. NR 35 TC 1 Z9 1 U1 6 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 858 EP 865 DI 10.1094/PDIS-10-14-1004-RE PG 8 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000015 ER PT J AU Rockey, W Potnis, N Timilsina, S Hong, JC Vallad, GE Jones, JB Norman, DJ AF Rockey, W. Potnis, N. Timilsina, S. Hong, J. C. Vallad, G. E. Jones, J. B. Norman, D. J. TI Multilocus Sequence Analysis Reveals Genetic Diversity in Xanthomonads Associated With Poinsettia Production SO PLANT DISEASE LA English DT Article ID GENUS XANTHOMONAS; STRAINS; TAXONOMY; AFLP; PCR; RELATEDNESS; PATHOVARS; INFERENCE; MODEL AB Xanthomonas axonopodis pv. poinsettiicola is traditionally identified as the primary causal agent of bacterial leaf spot on poinsettia (family Euphorbiaceae). Sixty-seven strains of xanthomonads isolated from lesions associated with several species within the family Euphorbiaceae were collected over a 64-year period. The pathogenicity of these strains was compared on several potential hosts and they were analyzed by multilocus sequence analysis (MLSA) using six housekeeping genes. The 67 Xanthomonas strains associated with poinsettia production were separated into three distinct clades based on MLSA. The first clade identified contained the X. axonopodis pv. poinsettiicola reference strain (LMG849(PT)). A second clade was more closely related to X. hortorum pv. pelargonii (LMG7314(PT)) and the third clade contained the X. codiaei type strain (LMG8678(T)). This analysis indicated that there may also be other closely related pathovars or species of Xanthomonas that can infect poinsettia. Strains from the three clades could not be distinguished by symptoms or virulence on poinsettia plants. Strains capable of infecting geranium were found in all three clades, although the extent of leaf spot formation and number of systemic infections were significantly less than those produced by X. hortorum pv. pelargonii strains, typically the main causal agent of bacterial leaf spot on geranium. Clade III also contained strains isolated from zebra plant (Aphelandra squarrosa, family Acanthaceae), which is a newly recognized host for X. codiaei and X. axonopodis pv. poinsettiicola. Xanthomonas leaf spot is a serious threat to poinsettia production that can be caused by several Xanthomonas spp. that can infect different ornamental plant hosts. It is imperative that growers maintain a strict sanitation program because reservoirs of inoculum can occur on a number of ornamental hosts. C1 [Rockey, W.; Potnis, N.; Timilsina, S.; Jones, J. B.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Hong, J. C.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Vallad, G. E.] Univ Florida, Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. [Norman, D. J.] Univ Florida, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. RP Norman, DJ (reprint author), Univ Florida, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. EM djn@ufl.edu FU Fred C. Gloeckner Foundation; University of Florida Institute of Food and Agricultural Sciences FX This research was funded by the Fred C. Gloeckner Foundation and the University of Florida Institute of Food and Agricultural Sciences. NR 28 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 874 EP 882 DI 10.1094/PDIS-08-14-0867-RE PG 9 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000017 ER PT J AU Green, MJ Daily, EL Davis, RE AF Green, M. J. Daily, E. L. Davis, R. E. TI The Lilac Cultivar Syringa 'Charisma' is a New Host for 'Candidates Phytoplasma pruni,' the Group 16SrIII, Subgroup A, Phytoplasma SO PLANT DISEASE LA English DT News Item ID X-DISEASE; CLASSIFICATION C1 [Green, M. J.] Canadian Food Inspect Agcy, Sidney Lab, Ctr Plant Hlth, North Saanich, BC V8L 1H3, Canada. [Daily, E. L.; Davis, R. E.] USDA ARS, MPPL, Beltsville, MD 20705 USA. RP Green, MJ (reprint author), Canadian Food Inspect Agcy, Sidney Lab, Ctr Plant Hlth, North Saanich, BC V8L 1H3, Canada. NR 4 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 886 EP 887 DI 10.1094/PDIS-12-14-1246-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000021 ER PT J AU Mulvey, ARL Hambleton, S AF Mulvey, Alaska. R. L. Hambleton, S. TI Stem Rust of Highbush-Cranberry (Viburnum edule) Caused by Puccinia linkii near Juneau, Alaska SO PLANT DISEASE LA English DT News Item C1 [Mulvey, Alaska. R. L.] USDA ARS, Forest Hlth Protect, Juneau, AK 99801 USA. [Hambleton, S.] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada. RP Mulvey, ARL (reprint author), USDA ARS, Forest Hlth Protect, Juneau, AK 99801 USA. NR 4 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 893 EP 893 DI 10.1094/PDIS-09-14-0987-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000037 ER PT J AU Warfield, CY Clemens, K Adkins, S AF Warfield, C. Y. Clemens, K. Adkins, S. TI First Report of Tomato chlorotic spot virus on Annual Vinca (Catharatzthus roseus) in the United States SO PLANT DISEASE LA English DT News Item C1 [Warfield, C. Y.; Clemens, K.] Ball Hort Co, West Chicago, IL 60185 USA. [Adkins, S.] USDA ARS, Ft Pierce, FL 34945 USA. RP Warfield, CY (reprint author), Ball Hort Co, West Chicago, IL 60185 USA. NR 5 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2015 VL 99 IS 6 BP 895 EP 896 DI 10.1094/PDIS-12-14-1269-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CQ8NW UT WOS:000360866000045 ER PT J AU Han, M Zhao, CY Feng, G Yan, YY Sheng, Y AF Han, Ming Zhao, Chengyi Feng, Gary Yan, Yingyu Sheng, Yu TI Evaluating the Effects of Mulch and Irrigation Amount on Soil Water Distribution and Root Zone Water Balance Using HYDRUS-2D SO WATER LA English DT Article ID SUBSURFACE DRIP IRRIGATION; PLASTIC MULCH; COTTON ROOT; WETTING PATTERNS; POINT SOURCES; YIELD; MODEL; FERTIGATION; SYSTEM; CHINA AB Water scarcity is the most critical constraint for sustainable cotton production in Xinjiang Province, northwest China. Drip irrigation under mulch is a major water-saving irrigation method that has been widely practiced for cotton production in Xinjiang. The performance of such an irrigation system should be evaluated for proper design and management. Therefore, a field experiment and a simulation study were conducted to (1) determine a modeling approach that can be applied to manage drip irrigation under mulch for cotton production in this region; and (2) examine the effects of irrigation amount and mulch on soil water distribution and root zone water balance components. In the experiment, four irrigation treatments were used: T1, 166.5 m(3); T2, 140.4 m(3); T3, 115.4 m(3); and T4: 102.3 m(3). The HYDRUS-2D model was calibrated, validated, and applied with the data obtained in this experiment. Soil water balance in the 0-70 cm soil profile was simulated. Results indicate that the observed soil water content and the simulated results obtained with HYDRUS-2D are in good agreement. The radius of the wetting pattern, root water uptake, and evaporation decreased as the amount of irrigation was reduced from T1 to T4, while a lot of stored soil water in the root zone was utilized and a huge amount of water was recharged from the layer below 70 cm to compensate for the decrease in irrigation amount. Mulch significantly reduced evaporation by 11.7 mm and increased root water uptake by 11.2 mm. Our simulation study suggests that this model can be applied to provide assistance in designing drip irrigation systems and developing irrigation strategies. C1 [Han, Ming; Zhao, Chengyi; Sheng, Yu] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Oasis Desert & Ecol, Urumqi 830011, Peoples R China. [Han, Ming] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Feng, Gary] ARS, Genet & Precis Agr Res Unit, USDA, Mississippi State, MS 39762 USA. [Yan, Yingyu] Xinjiang Monitoring Stn Water & Soil Conservat, Urumqi 830000, Peoples R China. RP Zhao, CY (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Oasis Desert & Ecol, Urumqi 830011, Peoples R China. EM dustming@gmail.com; zcy@ms.xjb.ac.cn; Gary.Feng@ars.usda.gov; yyy_2009@126.com; shengyu@ms.xjb.ac.cn FU Key National Natural Science Foundation [U14003281, 4117095]; National Project of China [2013BAC10B01]; Project SuMaRiO of BMBF (German Federal Ministry of Science and Technology)-Sustainable Management of River Oases along the Tarim River FX The study was supported by the Key National Natural Science Foundation (U14003281), (4117095) and National Project (No. 2013BAC10B01) of China and Project SuMaRiO of BMBF (German Federal Ministry of Science and Technology)-Sustainable Management of River Oases along the Tarim River. NR 47 TC 1 Z9 1 U1 10 U2 35 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD JUN PY 2015 VL 7 IS 6 BP 2622 EP 2640 DI 10.3390/w7062622 PG 19 WC Water Resources SC Water Resources GA CR0SO UT WOS:000361033700004 ER PT J AU Walston, LJ Najjar, SJ LaGory, KE Drake, SM AF Walston, Leroy J. Najjar, Stephen J. LaGory, Kirk E. Drake, Sean M. TI SPATIAL ECOLOGY OF BLANDING'S TURTLES (EMYDOIDEA BLANDINGII) IN SOUTHCENTRAL NEW HAMPSHIRE WITH IMPLICATIONS TO ROAD MORTALITY SO HERPETOLOGICAL CONSERVATION AND BIOLOGY LA English DT Article DE activity; behavior; compositional analysis; conservation; home range; minimum convex polygon ID FRESH-WATER TURTLES; HABITAT USE; HOME-RANGE; SPECIES CONSERVATION; CENTRAL WISCONSIN; MOVEMENTS; LANDSCAPE; POPULATIONS; ONTARIO; WETLAND AB Understanding the spatial ecology and habitat requirements of rare turtle species and the factors that threaten their populations is important for the success of long-term conservation programs. We present results from an eight-year field study in which we used radiotelemetry to monitor the activity and habitat use of 22 adult (seven male, 15 female) Blanding's Turtles (Emydoidea blandingii) in southcentral New Hampshire. Female turtles had significantly larger home ranges (mean = 19.6 ha +/- 3.5 SE) than males (mean = 10.7 ha +/- 0.1 SE). Activity patterns varied by season, with activity increasing each month after hibernation until peaking in June, coinciding with the nesting season. Males selected emergent and scrub-shrub wetlands in each season, whereas females selected scrub-shrub wetlands in spring and ponds in summer and fall. We identified road mortality as a potentially important threat for this population because females had greater road densities within home ranges and crossed roads more frequently than males. We attribute differences in road density and road crossings between the sexes to the irregular long distance nest forays and roadside nest site selection among females. The preservation of wetland networks and the implementation of measures to minimize road mortality are important considerations for the long-term persistence of this population. C1 [Walston, Leroy J.; LaGory, Kirk E.] Argonne Natl Lab, Div Environm Sci, Argonne, IL 60439 USA. [Najjar, Stephen J.] New Boston Air Force Stn, New Boston, NH USA. [Drake, Sean M.] US Forest Serv, White Mt Natl Forest, Gorham, NH USA. RP Walston, LJ (reprint author), Argonne Natl Lab, Div Environm Sci, 9700 S Cass Ave, Argonne, IL 60439 USA. EM lwalston@anl.gov FU United States Department of Defense; United States Air Force, through the United States Department of Energy [DEAC02-06CH11357] FX We thank the staff of the NBAFS for aiding in turtle collection. This work was supported under a military interdepartmental purchase request from the United States Department of Defense, United States Air Force, through the United States Department of Energy contract DEAC02-06CH11357. NR 38 TC 0 Z9 0 U1 5 U2 29 PU HERPETOLOGICAL CONSERVATION & BIOLOGY PI CORVALLIS PA C/O R BRUCE BURY, USGS FOREST & RANGELAND, CORVALLIS, OR 00000 USA SN 2151-0733 EI 1931-7603 J9 HERPETOL CONSERV BIO JI Herpetol. Conserv. Biol. PD JUN PY 2015 VL 10 IS 1 BP 284 EP 296 PG 13 WC Zoology SC Zoology GA CQ1QK UT WOS:000360373100022 ER PT J AU Lucas, JS Loeb, SC Jodice, PGR AF Lucas, Jessica S. Loeb, Susan C. Jodice, Patrick G. R. TI Roost selection by Rafinesque's big-eared bats (Corynorhinus rafinesquii) in a pristine habitat at three spatial scales SO ACTA CHIROPTEROLOGICA LA English DT Article DE bottomland hardwoods; predator avoidance; Rafinesque's big-eared bat; roost selection; roost fidelity ID MYOTIS MYOTIS AUSTRORIPARIUS; BOTTOMLAND HARDWOOD FORESTS; FEMALE EASTERN PIPISTRELLES; BROWN BATS; EPTESICUS-FUSCUS; SOUTHEASTERN MYOTIS; SITE SELECTION; DWELLING BAT; TREE ROOSTS; LANDSCAPE AB Although several studies have described roost use by Rafinesque's big-eared bats (Corynorhinus rafinesquii), few studies have examined roost selection. We examined roost use and selection by Rafinesque's big-eared bat at the tree, stand, and landscape scales during the maternity season in pristine old-growth habitat in the Coastal Plain of South Carolina. We located 43 roosts (14 maternity, 29 solitary) through cavity searches and radio-telemetry. Maternity colonies and solitary individuals selected roosts based on similar characteristics. The best model explaining roost selection by all bats included tree and stand characteristics; landscape factors had little influence on roost use. Bats selected large diameter trees in areas with a high density of trees with cavities in the surrounding area. Most roosts (67.4%) were in water tupelo (Nyssa aquatica) in semi-permanently flooded and saturated areas. Half of maternity roost cavities had upper bole openings whereas only 25.8% of solitary roosts had upper bole openings. Bats that roosted with maternity groups stayed in roosts for significantly shorter periods of time (1.3 +/- 0.1 days) and used significantly more roost trees (5.0 +/- 0.6 roosts) than adult males (3.8 +/- 1.10 days, 2.3 +/- 0.4 roosts, respectively). Maternity colony use of cavities with upper bole openings and shorter residency times suggest that predator avoidance may have been an important factor governing roosting behavior of maternity colonies in this area. Our results suggest that retention of large diameter, hollow trees in wetland areas will benefit Rafinesque's big-eared bat individuals and maternity colonies in this area. C1 [Lucas, Jessica S.] Clemson Univ, Sch Agr Forestry & Environm Sci, Clemson, SC 29634 USA. [Lucas, Jessica S.] Clemson Univ, US Geol Survey South Carolina Cooperat Fish, Clemson, SC 29634 USA. [Lucas, Jessica S.] Clemson Univ, Wildlife Res Unit, Clemson, SC 29634 USA. [Loeb, Susan C.] Clemson Univ, USDA Forest Serv, Southern Res Stn, Clemson, SC 29634 USA. [Jodice, Patrick G. R.] Clemson Univ, US Geol Survey, South Carolina Cooperat Fish & Wildlife Res Unit, Clemson, SC 29634 USA. RP Loeb, SC (reprint author), Clemson Univ, USDA Forest Serv, Southern Res Stn, Clemson, SC 29634 USA. EM sloeb@fs.fed.us FU Congaree National Park; USDA Forest Service, Southern Research Station FX Funding for this project was provided by Congaree National Park and the USDA Forest Service, Southern Research Station. We thank B. Hulslander, T. Yednock, T. Thom, and the rest of the Congaree National Park staff for all their logistical assistance. We thank M. Hobby, B. Smith, and S. Walters for field assistance and E. Winters for GIS and mapping assistance. B. L. Brown, J. S. Johnson, and J. C. Jones provided many useful comments on earlier drafts of this manuscript. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 59 TC 0 Z9 0 U1 2 U2 18 PU MUSEUM & INST ZOOLOGY PAS-POLISH ACAD SCIENCES PI WARSAW PA WILCZA STREET 64, 00-679 WARSAW, POLAND SN 1508-1109 EI 1733-5329 J9 ACTA CHIROPTEROL JI Acta Chiropt. PD JUN PY 2015 VL 17 IS 1 BP 131 EP 141 DI 10.3161/15081109ACC2015.17.1.011 PG 11 WC Zoology SC Zoology GA CO6WU UT WOS:000359298700011 ER PT J AU Crossey, LC Karlstrom, KE Dorsey, R Pearce, J Wan, E Beard, LS Asmerom, Y Polyak, V Crow, RS Cohen, A Bright, J Pecha, ME AF Crossey, L. C. Karlstrom, K. E. Dorsey, R. Pearce, J. Wan, E. Beard, L. S. Asmerom, Y. Polyak, V. Crow, R. S. Cohen, A. Bright, J. Pecha, M. E. TI Importance of groundwater in propagating downward integration of the 6-5 Ma Colorado River system: Geochemistry of springs, travertines, and lacustrine carbonates of the Grand Canyon region over the past 12 Ma SO GEOSPHERE LA English DT Article ID GULF-OF-CALIFORNIA; PLIOCENE BOUSE FORMATION; U-PB GEOCHRONOLOGY; SAN-FRANCISCO BAY; PALEOCLIMATE CHANGES; ESTUARINE SEDIMENTS; SOUTHERN NEVADA; WESTERN ARIZONA; ANDREAS FAULT; NORTH-AMERICA AB We applied multiple geochemical tracers (Sr-87/Sr-86, [Sr], delta C-13, and delta O-18) to waters and carbonates of the lower Colorado River system to evaluate its paleohydrology over the past 12 Ma. Modern springs in Grand Canyon reflect mixing of deeply derived (endogenic) fluids with meteoric (epigenic) recharge. Travertine (<1 Ma) and speleothems (2-4 Ma) yield Sr-87/Sr-86 and delta C-13 and delta O-18 values that overlap with associated water values, providing justification for use of carbonates as a proxy for the waters from which they were deposited. The Hualapai Limestone (12-6 Ma) and Bouse Formation (5.6-4.8 Ma) record paleohydrology immediately prior to and during integration of the Colorado River. The Hualapai Limestone was deposited from 12 Ma (new ash age) to 6 Ma; carbonates thicken eastward to similar to 210 m toward the Grand Wash fault, suggesting that deposition was synchronous with fault slip. A fanning-dip geometry is suggested by correlation of ashes between subbasins using tephrochronology. New detrital-zircon ages are consistent with the "Muddy Creek constraint," which posits that Grand Wash Trough was internally drained prior to 6 Ma, with limited or no Colorado Plateau detritus, and that Grand Wash basin was sedimentologically distinct from Gregg and Temple basins until after 6 Ma. New isotopic data from Hualapai Limestone of Grand Wash basin show values and ranges of Sr-87/Sr-86, delta C-13, and delta O-18 that are similar to Grand Canyon springs and travertines, suggesting a long-lived spring-fed lake/marsh system sourced from western Colorado Plateau groundwater. Progressive up-section decrease in Sr-87/Sr-86 and delta C-13 and increase in delta O-18 in the uppermost 50 m of the Hualapai Limestone indicate an increase in meteoric water relative to endogenic inputs, which we interpret to record progressively increased input of high-elevation Colorado Plateau groundwater from ca. 8 to 6 Ma. Grand Wash, Hualapai, Gregg, and Temple basins, although potentially connected by groundwater, were hydrochemically distinct basins before ca. 6 Ma. The Sr-87/Sr-86, delta C-13, and delta O-18 chemostratigraphic trends are compatible with a model for downward integration of Hualapai basins by groundwater sapping and lake spillover. The Bouse Limestone (5.6-4.8 Ma) was also deposited in several hydrochemically distinct basins separated by bedrock divides. Northern Bouse basins (Cottonwood, Mojave, Havasu) have carbonate chemistry that is nonmarine. The Sr-87/Sr-86 data suggest that water in these basins was derived from mixing of high-Sr-87/Sr-86 Lake Hualapai waters with lower-Sr-87/Sr-86, first-arriving "Colorado River" waters. Covariation trends of delta C-13 and delta O-18 suggest that newly integrated Grand Wash, Gregg, and Temple basin waters were integrated downward to the Cottonwood and Mojave basins at ca. 5-6 Ma. Southern, potentially younger Bouse basins are distinct hydrochemically from each other, which suggests incomplete mixing during continued downward integration of internally drained basins. Bouse carbonates display a southward trend toward less radiogenic Sr-87/Sr-86 values, higher [Sr], and heavier delta O-18 that we attribute to an increased proportion of Colorado River water through time plus increased evaporation from north to south. The delta C-13 and delta O-18 trends suggest alternating closed and open systems in progressively lower (southern) basins. We interpret existing data to permit the interpretation that the southernmost Blythe basin may have had intermittent mixing with marine water based on delta C-13 and delta O-18 covariation trends, sedimentology, and paleontology. [Sr] versus Sr-87/Sr-86 modeling suggests that southern Blythe basin Sr-87/Sr-86 values of similar to 0.710-0.711 could be produced by 25%-75% seawater mixed with river water (depending on [Sr] assumptions) in a delta-marine estuary system. We suggest several refinements to the "lake fill-and-spill" downward integration model for the Colorado River: (1) Lake Hualapai was fed by western Colorado Plateau groundwater from 12 to 8 Ma; (2) high-elevation Colorado Plateau groundwater was progressively introduced to Lake Hualapai from ca. 8 to 6 Ma; (3) Colorado River water arrived at ca. 5-6 Ma; and (4) the combined inputs led to downward integration by a combination of groundwater sapping and sequential lake spillover that first delivered Colorado Plateau water and detritus to the Salton Trough at ca. 5.3 Ma. We propose that the groundwater sapping mechanism strongly influenced lake evolution of the Hualapai and Bouse Limestones and that groundwater flow from the Colorado Plateau to Grand Wash Trough led to Colorado River integration. C1 [Crossey, L. C.; Karlstrom, K. E.; Asmerom, Y.; Polyak, V.; Crow, R. S.] Univ New Mexico, Dept Earth & Planetary Sci, Albuquerque, NM 87131 USA. [Dorsey, R.] 1272 Univ Oregon, Dept Geol Sci, Eugene, OR 97403 USA. [Pearce, J.] US Forest Serv, Grand Mesa Uncompahgre & Gunnison Natl Forests, Paonia, CO 81428 USA. [Wan, E.] US Geol Survey, Menlo Pk, CA 94025 USA. [Beard, L. S.] US Geol Survey, Flagstaff, AZ 86001 USA. [Cohen, A.; Bright, J.; Pecha, M. E.] Univ Arizona, Dept Geosci, Tucson, AZ 85721 USA. RP Crossey, LC (reprint author), Univ New Mexico, Dept Earth & Planetary Sci, Albuquerque, NM 87131 USA. OI Crossey, Laura/0000-0001-6237-8023 FU National Science Foundation (NSF) from Hydrologic Sciences Program [EAR-0838575, EAR-1119629, EAR-1242028, EAR-1348007]; NSF GK-12 program [DGE-0538396]; NSF of the Arizona LaserChron Center [EAR-1338583] FX This project benefited from partial financial support from National Science Foundation (NSF) grants EAR-0838575 from the Hydrologic Sciences Program, EAR-1119629, -1242028, and -1348007 from the Tectonics Program, and DGE-0538396 from the NSF GK-12 program. For tephrochronology, Holly Olson performed the laboratory processing and petrography, Dave Wahl conducted the instrumental and computer analysis, and Elmira Wan interpreted the data. Detrital-zircon samples were run by Mark Pecha and the University of Arizona LaserChron laboratory, and we acknowledge NSF grant EAR-1338583 for support of the Arizona LaserChron Center. W.R. Dickinson helped analyze and interpret the detrital-zircon data. Mark Holland created the detrital-zircon figure, and Jason Ricketts helped with drafting of other figures. We thank Viorel Atudori and the University of New Mexico Stable Isotope Laboratory for stable isotope data. The manuscript benefited from discussions and reviews from W.R Dickinson, David Miller, and an anonymous reviewer, as well as from Guest Associate Editor Andres Aslan. NR 99 TC 10 Z9 10 U1 3 U2 21 PU GEOLOGICAL SOC AMER, INC PI BOULDER PA PO BOX 9140, BOULDER, CO 80301-9140 USA SN 1553-040X J9 GEOSPHERE JI Geosphere PD JUN PY 2015 VL 11 IS 3 BP 660 EP 682 DI 10.1130/GES01073.1 PG 23 WC Geosciences, Multidisciplinary SC Geology GA CO9FP UT WOS:000359479400007 ER PT J AU Anekwe, TD Rahkovsky, I AF Anekwe, Tobenna D. Rahkovsky, Iiya TI The Association Between Food Prices and the Blood Glucose Level of US Adults With Type 2 Diabetes SO REVISTA PANAMERICANA DE SALUD PUBLICA-PAN AMERICAN JOURNAL OF PUBLIC HEALTH LA Spanish DT Article ID TAXES; DIET; CONSUMPTION; VEGETABLES; WEIGHT; FRUITS; HEALTH AB Objectives. We estimated the association between the price of healthy and less-healthy food groups and blood sugar among US adults with type 2 diabetes. Methods. We linked 1999-2006 National Health and Nutrition Examination Survey health information to food prices contained in the Quarterly Food-at-Home Price Database. We regressed blood sugar levels on food prices from the previous calendar quarter, controlling for market region and a range of other covariates. We also examined whether the association between food prices and blood sugar varies among different income groups. Results. The prices of produce and low-fat dairy foods were associated with blood sugar levels of people with type 2 diabetes. Specifically, higher prices for produce and low-fat dairy foods were associated with higher levels of glycated hemoglobin and fasting plasma glucose 3 months later. Food prices had a greater association with blood sugar for low-income people than for higherincome people, and in the expected direction. Conclusions. Higher prices of healthy foods were associated with increased blood sugar among people with type 2 diabetes. The association was especially pronounced among low-income people with type 2 diabetes. C1 [Anekwe, Tobenna D.; Rahkovsky, Iiya] USDA, Serv Invest Econ, Washington, DC 20250 USA. RP Anekwe, TD (reprint author), USDA, Serv Invest Econ, Washington, DC 20250 USA. EM tanekwe@ers.usda.gov NR 38 TC 0 Z9 0 U1 1 U2 3 PU PAN AMER HEALTH ORGANIZATION PI WASHINGTON PA 525 23RD ST NW, WASHINGTON, DC 20037 USA SN 1020-4989 J9 REV PANAM SALUD PUBL JI Rev. Panam. Salud Publica PD JUN PY 2015 VL 37 IS 6 BP 444 EP 452 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CO7CI UT WOS:000359314700012 PM 26245182 ER PT J AU Ford, WI Fox, JF AF Ford, William I. Fox, James F. TI Isotope-based Fluvial Organic Carbon (ISOFLOC) Model: Model formulation, sensitivity, and evaluation SO WATER RESOURCES RESEARCH LA English DT Article ID INORGANIC CARBON; HEADWATER STREAMS; NUTRIENT GRADIENT; STABLE-ISOTOPES; GROWTH-RATE; SEDIMENT; MATTER; NITROGEN; DECOMPOSITION; FRACTIONATION AB Watershed-scale carbon budgets remain poorly understood, in part due to inadequate simulation tools to assess in-stream carbon fate and transport. A new numerical model termed ISOtope-based FLuvial Organic Carbon (ISOFLOC) is formulated to simulate the fluvial organic carbon budget in watersheds where hydrologic, sediment transport, and biogeochemical processes are coupled to control benthic and transported carbon composition and flux. One ISOFLOC innovation is the formulation of new stable carbon isotope model subroutines that include isotope fractionation processes in order to estimate carbon isotope source, fate, and transport. A second innovation is the coupling of transfers between carbon pools, including algal particulate organic carbon, fine particulate and dissolved organic carbon, and particulate and dissolved inorganic carbon, to simulate the carbon cycle in a comprehensive manner beyond that of existing watershed water quality models. ISOFLOC was tested and verified in a low-gradient, agriculturally impacted stream. Results of a global sensitivity analysis suggested the isotope response variable had unique sensitivity to the coupled interaction between fluvial shear resistance of algal biomass and the concentration of dissolved inorganic carbon. Model calibration and validation suggested good agreement at event, seasonal, and annual timescales. Multiobjective uncertainty analysis suggested inclusion of the carbon stable isotope routine reduced uncertainty by 80% for algal particulate organic carbon flux estimates. C1 [Ford, William I.] USDA ARS, Columbus, OH USA. [Fox, James F.] Univ Kentucky, Civil Engn, Lexington, KY 40506 USA. RP Fox, JF (reprint author), Univ Kentucky, Civil Engn, Lexington, KY 40506 USA. EM james.fox@uky.edu FU University of Kentucky Department of Civil Engineering; National Science Foundation [0918856]; Kentucky Science & Engineering Foundation [2687-RDE-015] FX We thank Harry Rowe for analysis of the elemental data and numerous undergraduate and graduate research associates that assisted with data collection and analysis. We thank the University of Kentucky Department of Civil Engineering for partial funding of the graduate student. We gratefully acknowledge financial support of this research under National Science Foundation Award 0918856 and Kentucky Science & Engineering Foundation Award 2687-RDE-015. Please feel free to contact Fox in order to use the data in this paper. NR 75 TC 2 Z9 2 U1 5 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD JUN PY 2015 VL 51 IS 6 BP 4046 EP 4064 DI 10.1002/2015WR016999 PG 19 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA CN3CK UT WOS:000358301200010 ER PT J AU de Carvalho, LM Benda, ND Vaughan, MM Cabrera, AR Hung, K Cox, T Abdo, Z Allen, LH Teal, PEA AF de Carvalho, Luciana Marques Benda, Nicole D. Vaughan, Martha M. Cabrera, Ana R. Hung, Kaddie Cox, Thomas Abdo, Zaid Allen, L. Hartwell Teal, Peter E. A. TI Mi-1-Mediated Nematode Resistance in Tomatoes is Broken by Short-Term Heat Stress but Recovers Over Time SO JOURNAL OF NEMATOLOGY LA English DT Article DE heat stress; Meloidogyne incognita; Mi-1 gene; resistance; root-knot nematode; Solanum lycopersicum ID ROOT-KNOT NEMATODES; PLANT-PARASITIC NEMATODES; MELOIDOGYNE-INCOGNITA; SOIL TEMPERATURES; MI GENE; GENOTYPES; ROOTSTOCKS; PCR; EXPRESSION; INFECTION AB Tomato (Solanum lycopersicum L.) is among the most valuable agricultural products, but Meloidogyne spp. (root-knot nematode) infestations result in serious crop losses. In tomato, resistance to root-knot nematodes is controlled by the gene Mi-1, but heat stress interferes with Mi-1-associated resistance. Inconsistent results in published field and greenhouse experiments led us to test the effect of short-term midday heat stress on tomato susceptibility to Meloidogyne incognita race 1. Under controlled day/night temperatures of 25 degrees C/21 degrees C, 'Amelia', which was verified as possessing the Mi-1 gene, was deemed resistant (4.1 +/- 0.4 galls/plant) and Rutgers, which does not possess the Mi-1 gene, was susceptible (132 +/- 9.9 galls/plant) to M. incognita infection. Exposure to a single 3 hr heat spike of 35 degrees C was sufficient to increase the susceptibility of 'Amelia' but did not affect Rutgers. Despite this change in resistance, Mi-1 gene expression was not affected by heat treatment, or nematode infection. The heat-induced breakdown of Mi-1 resistance in 'Amelia' did recover with time regardless of additional heat exposures and M. incognita infection. These findings would aid in the development of management strategies to protect the tomato crop at times of heightened M. incognita susceptibility. C1 [de Carvalho, Luciana Marques] EMBRAPA Coastal Tablelands, Aracaju, SE, Brazil. [Benda, Nicole D.; Cabrera, Ana R.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Vaughan, Martha M.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. [Hung, Kaddie; Cox, Thomas; Allen, L. Hartwell; Teal, Peter E. A.] ARS, Ctr Med & Vet Entomol, USDA, Gainesville, FL USA. [Abdo, Zaid] ARS, Area Directors Off, USDA, Athens, GA USA. ARS, CMAVE, USDA, Gainesville, FL USA. RP de Carvalho, LM (reprint author), EMBRAPA Coastal Tablelands, Aracaju, SE, Brazil. EM nbenda@gmail.com RI Abdo, Zaid/E-5176-2017 OI Abdo, Zaid/0000-0002-8272-7734 FU USDA FX This research was funded by the USDA. NR 51 TC 0 Z9 0 U1 3 U2 18 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2015 VL 47 IS 2 BP 133 EP 140 PG 8 WC Zoology SC Zoology GA CO2MI UT WOS:000358990100006 ER PT J AU Howland, AD Skinkis, PA Wilson, JH Riga, E Pinkerton, JN Schreiner, RP Zasada, IA AF Howland, Amanda D. Skinkis, Patricia A. Wilson, John H. Riga, Ekaterini Pinkerton, John N. Schreiner, R. Paul Zasada, Inga A. TI Impact of Grapevine (Vitis vinifera) Varieties on Reproduction of the Northern Root- Knot Nematode (Meloidogyne hapla) SO JOURNAL OF NEMATOLOGY LA English DT Article DE host-parasite interaction; host status; M. hapla; microplots; own-rooted; Washington ID PLANT-PARASITIC NEMATODES; ROOTSTOCKS; PENETRATION; RESISTANCE; INFECTION; DENSITIES; TOLERANCE; ARENARIA; CLIMATE; GROWTH AB One of the most commonly encountered plant-parasitic nematodes in eastern Washington Vitis vinifera vineyards is Meloidogyne hapla; however, limited research exists on the impact of this nematode on V. vinifera. The objectives of this research were to determine the impact of M. hapla on Chardonnay and Cabernet Sauvignon vine establishment and to determine the host status of V. vinifera varieties/clones predominantly grown in Washington to M. hapla. In a microplot experiment, Chardonnay and Cabernet Sauvignon vines were planted into soil inoculated with different densities of M. hapla; population dynamics of M. hapla and vine performance were monitored over 3 yr. In greenhouse experiments, several clones representing five V. vinifera varieties, Chardonnay, Riesling, Cabernet Sauvignon, Merlot, and Syrah, were evaluated as hosts for M. hapla. In both microplot and greenhouse experiments, white varieties were significantly better hosts than red varieties. In the greenhouse experiments, Chardonnay and Riesling had 40% higher reproduction factor values than Syrah and Merlot, however, all varieties/clones screened were good hosts for M. hapla (reproduction factors. 3). In the microplot experiment, M. hapla eggs/g root were 4.5 times greater in Chardonnay compared to Cabernet Sauvignon 3 yr after planting but there was no evident impact of M. hapla on vine establishment. C1 [Howland, Amanda D.; Skinkis, Patricia A.] Oregon State Univ, Dept Hort, Corvallis, OR 97330 USA. [Wilson, John H.; Riga, Ekaterini] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Pinkerton, John N.; Schreiner, R. Paul; Zasada, Inga A.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Howland, AD (reprint author), Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97330 USA. EM inga.Zasada@ars.usda.gov FU Northwest Center for Small Fruit Research; Washington Grape & Wine Research Program FX This research was funded by the Northwest Center for Small Fruit Research and the Washington Grape & Wine Research Program. The authors thank Marj Lauer, Duncan Kroese, Amy Peetz, and Mariella Ballato for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 34 TC 1 Z9 1 U1 2 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2015 VL 47 IS 2 BP 141 EP 147 PG 7 WC Zoology SC Zoology GA CO2MI UT WOS:000358990100007 PM 26170476 ER PT J AU Wubben, MJ Gavilano, L Baum, TJ Davis, EL AF Wubben, Martin J. Gavilano, Lily Baum, Thomas J. Davis, Eric L. TI Sequence and Spatiotemporal Expression Analysis of CLE-Motif Containing Genes from the Reniform Nematode (Rotylenchulus reniformis Linford & Oliveira) SO JOURNAL OF NEMATOLOGY LA English DT Article DE CLE; effector; host-parasite relationship; reniform nematode; Rotylenchulus ID PLANT-PARASITIC NEMATODE; ESOPHAGEAL GLAND-CELLS; HETERODERA-GLYCINES; GLOBODERA-ROSTOCHIENSIS; ARABIDOPSIS-THALIANA; EFFECTOR PROTEINS; IDENTIFICATION; PEPTIDES; HOST; SIMILARITY AB The reniform nematode, Rotylenchulus reniformis, is a sedentary semi-endoparasitic species with a host range that encompasses more than 77 plant families. Nematode effector proteins containing plant-ligand motifs similar to CLAVATA3/ESR (CLE) peptides have been identified in the Heterodera, Globodera, and Meloidogyne genera of sedentary endoparasites. Here, we describe the isolation, sequence analysis, and spatiotemporal expression of three R. reniformis genes encoding putative CLE motifs named Rr-cle-1, Rr-cle-2, and Rr-cle-3. The Rr-cle cDNAs showed >98% identity with each other and the predicted peptides were identical with the exception of a short stretch of residues at the carboxy(C)-terminus of the variable domain (VD). Each RrCLE peptide possessed an amino-terminal signal peptide for secretion and a single C-terminal CLE motif that was most similar to Heterodera CLE motifs. Aligning the Rr-cle cDNAs with their corresponding genomic sequences showed three exons with an intron separating the signal peptide from the VD and a second intron separating the VD from the CLE motif. An alignment of the RrCLE1 peptide with Heterodera glycines and Heterodera schachtii CLE proteins revealed a high level of homology within the VD region associated with regulating in planta trafficking of the processed CLE peptide. Quantitative RT-PCR (qRT-PCR) showed similar expression profiles for each Rr-cle transcript across the R. reniformis life-cycle with the greatest transcript abundance being in sedentary parasitic female nematodes. In situ hybridization showed specific Rr-cle expression within the dorsal esophageal gland cell of sedentary parasitic females. C1 [Wubben, Martin J.] USDA ARS, Crop Sci Res Lab, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. [Wubben, Martin J.; Gavilano, Lily] Mississippi State Univ, Dept Plant & Soil Sci, Starkville, MS USA. [Baum, Thomas J.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA USA. [Davis, Eric L.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. RP Wubben, MJ (reprint author), USDA ARS, Crop Sci Res Lab, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. EM Martin.Wubben@ars.usda.gov FU United Soybean Board FX This work was funded in part by a grant from the United Soybean Board to ELD, TJB, and MJW. NR 35 TC 3 Z9 3 U1 0 U2 8 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2015 VL 47 IS 2 BP 159 EP 165 PG 7 WC Zoology SC Zoology GA CO2MI UT WOS:000358990100010 PM 26170479 ER PT J AU Smelser, A Blanco, M Lubberstedt, T Schechert, A Vanous, A Gardner, C AF Smelser, Andrew Blanco, Michael Luebberstedt, Thomas Schechert, Axel Vanous, Adam Gardner, Candice TI Weighing in on a method to discriminate maize haploid from hybrid seed SO PLANT BREEDING LA English DT Article DE doubled haploid; sorting kernels; maize breeding ID INDUCTION AB The doubled haploid breeding method can produce maize inbred lines faster than traditional methods, but there are challenges associated with it. Sorting haploid from hybrid seed based on visual colour markers is time consuming and can be difficult due to colour inhibitors that obscure pigmentation needed to distinguish between haploid, hybrid and out-crossed seed. In this study, weight was evaluated as a method to sort haploid from hybrid seed. A first experiment utilized two families for analysis in a preliminary study. Eleven haploid and hybrid kernels from both families were weighed for a total of 44 experimental units. A second experiment was carried out using six families, using the same format as the previous, for 132 experimental units. Hybrid seed weighed significantly more than haploid seed in both experiments. However, the interaction between line and kernel type was significant in the second experiment. In conclusion, efficacy of sorting haploid from hybrid kernels based on weight depends on the genotypes involved. C1 [Smelser, Andrew; Blanco, Michael] USDA ARS, North Cent Reg Plant Intro Stn, Ames, IA 50011 USA. [Luebberstedt, Thomas] Iowa State Univ, KJ Frey Chair Agron, Ames, IA 50011 USA. [Schechert, Axel] Strube Res GmbH & Co, D-38387 Soellingen, Germany. [Vanous, Adam] Iowa State Univ, Dept Agron, Ames, IA USA. [Gardner, Candice] Iowa State Univ, USDA ARS, Plant Intro Res Unit, Ames, IA USA. RP Smelser, A (reprint author), USDA ARS, North Cent Reg Plant Intro Stn, 1305 State Ave, Ames, IA 50011 USA. EM asmelser@mbsgenetics.com FU USDA-ARS; Hatch Multistate Project [NC-7] FX The Agriculture Experiment Station Consulting Group from the Department of Statistics at Iowa State University for statistical support. Funding support from USDA-ARS, and Hatch Multistate Project NC-7. Gerald De La Fuente for the use of haploid and hybrid seed used in this study. NR 6 TC 1 Z9 1 U1 0 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2015 VL 134 IS 3 BP 283 EP 285 DI 10.1111/pbr.12260 PG 3 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA CO3NB UT WOS:000359063600004 ER PT J AU Krishnamurthy, P Lee, JD Ha, BK Chae, JH Song, JT Tsukamoto, C Singh, RJ Chung, G AF Krishnamurthy, Panneerselvam Lee, Jeong-Dong Ha, Bo-Keun Chae, Jong-Hyun Song, Jong Tae Tsukamoto, Chigen Singh, Ram J. Chung, Gyuhwa TI Genetic characterization of group A acetylsaponin-deficient mutants from wild soybean (Glycine soja Sieb. and Zucc.) SO PLANT BREEDING LA English DT Article DE group A saponins; C-22 sugar chain biosynthesis; soyasaponins; soybean breeding; Glycine soja; wild soybean ID MAX L. MERRILL; SAPONINS; SEED; POLYMORPHISM; COMPONENTS; INHERITANCE; SOFTWARE; ALLELE; PLANT AB Group A acetylsaponins are the main causative components for bitter and astringent tastes of soybean (Glycine max). In this study, we examined the genetic nature of the absence of group A acetylsaponins in 12 Korean wild soybean (Glycine soja) accessions. In all 12 accessions, the coding region (1431-bp) of Sg-1 locus was identical with Sg-1(a), which adds the xylose sugar moiety at the terminal position of the C-22 sugar chain of SS-A, except one nucleotide (G -> A change) at +948th position. This point mutation results in change of one amino acid from tryptophan (TGG) to stop codon (TGA). We observed that the mutated Sg-1 was controlled by a single recessive gene (sg-1(0-a1)). This gene was mapped between BARCSOYSSR_07_1561 and BARCSOYSSR_07_1598 on soybean chromosome 7. Our study demonstrated that the mutated Sg-1 gene in Korean wild soybeans is genetically different from those identified in Japanese soybean cultivar 'Kinusayaka' and wild soybean JP-36121. We believe that the new Sg-1 mutants can also be utilized to produce a new soybean variety without bitter and astringent properties. C1 [Krishnamurthy, Panneerselvam; Chung, Gyuhwa] Chonnam Natl Univ, Dept Biotechnol, Yeosu 550749, Chonnam, South Korea. [Krishnamurthy, Panneerselvam] Natl Acad Agr Sci, Dept Agr Biotechnol, Jeonju 560500, South Korea. [Lee, Jeong-Dong; Chae, Jong-Hyun; Song, Jong Tae] Kyungpook Natl Univ, Sch Appl Biosci, Taegu 702701, South Korea. [Ha, Bo-Keun] Chonnam Natl Univ, Div Plant Biotechnol, Kwangju 500757, South Korea. [Tsukamoto, Chigen] Iwate Univ, Dept Appl Biol Chem, Morioka, Iwate 0208550, Japan. [Singh, Ram J.] Univ Illinois, USDA ARS, Natl Soybean Res Lab, Urbana, IL 61801 USA. RP Chung, G (reprint author), Chonnam Natl Univ, Dept Biotechnol, Yeosu 550749, Chonnam, South Korea. EM chung@chonnam.ac.kr FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2010-0013600] FX This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013600). NR 23 TC 3 Z9 3 U1 2 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2015 VL 134 IS 3 BP 316 EP 321 DI 10.1111/pbr.12269 PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA CO3NB UT WOS:000359063600009 ER PT J AU Stansell, Z Cory, W Couillard, D Farnham, M AF Stansell, Zachary Cory, Wendy Couillard, David Farnham, Mark TI Collard landraces are novel sources of glucoraphanin and other aliphatic glucosinolates SO PLANT BREEDING LA English DT Article DE collard; glucosinolate; glucoraphanin; Brassica oleracea var. viridis; chemoprotection; phytonutrients ID CRUCIFEROUS VEGETABLES; BRASSICA-OLERACEA; HORTICULTURAL TRAITS; BROCCOLI; CANCER; SULFORAPHANE; PREVENTION; CULTIVARS; PROFILES; MUSTARD AB The glucosinolate make-up of the edible parts of some Brassica oleracea L. crops has been investigated previously, but the leafy-green collard (B. oleracea var. viridis) remains relatively unstudied on this topic. Due to this lack of information, a collection of US landraces was examined for glucosinolate content of leaves. The specific objectives of this examination were to compare levels of certain glucosinolates among the conserved collard landraces, identify any individuals with a distinct glucosinolate profile and determine the potential of collard as a target for chemoprotective-based plant breeding. During winter 2010/2011, 81 collard landraces, four other viridis and four collard cultivars were evaluated in the field and harvested leaves were assayed for glucosinolates. In a subsequent study, 19 selected landraces plus the cultivars were included in a repeat trial in 2012/2013. Eighteen collard landraces contained relatively high levels of glucoraphanin in leaves in both years, and three (designated G 32575, G 32580, G 32586 in the US National Plant Germplasm System) were found to contain glucoraphanin in excess of 9.5 mu mol/g DM. The examined landraces are rich sources of important aliphatic glucosinolates, previously thought to be most abundant in other B. oleracea vegetables. C1 [Stansell, Zachary; Couillard, David; Farnham, Mark] ARS, USDA, US Vegetable Lab, Charleston, SC 29414 USA. [Cory, Wendy] Coll Charleston, Dept Chem & Biochem, Charleston, SC 29424 USA. RP Stansell, Z (reprint author), ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM zachary.stansell@ars.usda.gov NR 28 TC 0 Z9 0 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2015 VL 134 IS 3 BP 350 EP 355 DI 10.1111/pbr.12263 PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA CO3NB UT WOS:000359063600014 ER PT J AU Slattery, RA Ort, DR AF Slattery, Rebecca A. Ort, Donald R. TI Photosynthetic Energy Conversion Efficiency: Setting a Baseline for Gauging Future Improvements in Important Food and Biofuel Crops SO PLANT PHYSIOLOGY LA English DT Review ID RADIATION-USE EFFICIENCY; DRY-MATTER PRODUCTION; MAIZE ZEA-MAYS; ARID TROPICAL ENVIRONMENT; ABSORBED SOLAR-RADIATION; MISCANTHUS X GIGANTEUS; GENETIC YIELD GAINS; ARACHIS-HYPOGAEA L; SUPER HYBRID RICE; WHEAT-GRAIN-YIELD AB The conversion efficiency (epsilon(c)) of absorbed radiation into biomass (MJ of dry matter per MJ of absorbed photosynthetically active radiation) is a component of yield potential that has been estimated at less than half the theoretical maximum. Various strategies have been proposed to improve epsilon(c), but a statistical analysis to establish baseline epsilon(c) levels across different crop functional types is lacking. Data from 164 published epsilon(c) studies conducted in relatively unstressed growth conditions were used to determine the means, greatest contributors to variation, and genetic trends in epsilon(c) across important food and biofuel crop species. epsilon(c) was greatest in biofuel crops (0.049-0.066), followed by C-4 food crops (0.046-0.049), C-3 nonlegumes (0.036-0.041), and finally C-3 legumes (0.028-0.035). Despite confining our analysis to relatively unstressed growth conditions, total incident solar radiation and average growing season temperature most often accounted for the largest portion of epsilon(c) variability. Genetic improvements in epsilon(c), when present, were less than 0.7% per year, revealing the unrealized potential of improving epsilon(c) as a promising contributing strategy to meet projected future agricultural demand. C1 [Slattery, Rebecca A.; Ort, Donald R.] USDA, Dept Plant Biol, Urbana, IL 61801 USA. [Slattery, Rebecca A.; Ort, Donald R.] USDA, Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.] USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Ort, DR (reprint author), USDA, Dept Plant Biol, Urbana, IL 61801 USA. EM d-ort@illinois.edu FU U.S. Department of Agriculture Agricultural Research Service FX This work was supported by the U.S. Department of Agriculture Agricultural Research Service. NR 214 TC 3 Z9 5 U1 5 U2 22 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2015 VL 168 IS 2 BP 383 EP + DI 10.1104/pp.15.00066 PG 32 WC Plant Sciences SC Plant Sciences GA CO6WR UT WOS:000359298200001 PM 25829463 ER PT J AU Zhang, NY Gibon, Y Wallace, JG Lepak, N Li, PH Dedow, L Chen, C So, YS Kremling, K Bradbury, PJ Brutnell, T Stitt, M Buckler, ES AF Zhang, Nengyi Gibon, Yves Wallace, Jason G. Lepak, Nicholas Li, Pinghua Dedow, Lauren Chen, Charles So, Yoon-Sup Kremling, Karl Bradbury, Peter J. Brutnell, Thomas Stitt, Mark Buckler, Edward S. TI Genome-Wide Association of Carbon and Nitrogen Metabolism in the Maize Nested Association Mapping Population SO PLANT PHYSIOLOGY LA English DT Article ID C-4 PHOTOSYNTHESIS; GENETIC ARCHITECTURE; ENZYME-ACTIVITIES; QUANTITATIVE RESISTANCE; ARABIDOPSIS-THALIANA; TOBACCO PLANTS; BUNDLE-SHEATH; LEAF-BLIGHT; EXPRESSION; INVERTASE AB Carbon (C) and nitrogen (N) metabolismare critical to plant growth and development and are at the basis of crop yield and adaptation. We performed high-throughput metabolite analyses on over 12,000 samples from the nested association mapping population to identify genetic variation in C and N metabolism in maize (Zea mays ssp. mays). All samples were grown in the same field and used to identify natural variation controlling the levels of 12 key C and N metabolites, namely chlorophyll a, chlorophyll b, fructose, fumarate, glucose, glutamate, malate, nitrate, starch, sucrose, total amino acids, and total protein, along with the first two principal components derived from them. Our genome-wide association results frequently identified hits with single-gene resolution. In addition to expected genes such as invertases, natural variation was identified in key C-4 metabolism genes, including carbonic anhydrases and a malate transporter. Unlike several prior maize studies, extensive pleiotropy was found for C and N metabolites. This integration of field-derived metabolite data with powerful mapping and genomics resources allows for the dissection of key metabolic pathways, providing avenues for future genetic improvement. C1 [Zhang, Nengyi; Wallace, Jason G.; Kremling, Karl; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Li, Pinghua; Dedow, Lauren; Brutnell, Thomas] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Chen, Charles; Kremling, Karl; Buckler, Edward S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Brutnell, Thomas] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Gibon, Yves; Stitt, Mark] Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany. [Lepak, Nicholas; Bradbury, Peter J.; Buckler, Edward S.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [So, Yoon-Sup] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Gibon, Y (reprint author), Univ Bordeaux, Inst Natl Rech Agron, UMR 1332, F-33883 Villenave Dornon, France. EM yves.gibon@bordeaux.inra.fr; mstitt@mpimp-golm.mpg.de; esb33@cornell.edu RI Brutnell, Thomas/M-2840-2013; OI Brutnell, Thomas/0000-0002-3581-8211; Wallace, Jason/0000-0002-8937-6543; Buckler, Edward/0000-0002-3100-371X FU National Science Foundation [DBI-0501700, DBI-0321467, DBI-0820619, IOS-1238014]; U.S. Department of Agriculture-Agricultural Research Service; Max Planck Society FX This work was supported by the National Science Foundation (grant nos. DBI-0501700, DBI-0321467, DBI-0820619, and IOS-1238014), the U.S. Department of Agriculture-Agricultural Research Service, and the Max Planck Society. NR 62 TC 9 Z9 9 U1 13 U2 82 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2015 VL 168 IS 2 BP 575 EP 583 DI 10.1104/pp.15.00025 PG 9 WC Plant Sciences SC Plant Sciences GA CO6WR UT WOS:000359298200016 PM 25918116 ER PT J AU Swetnam, TL Lynch, AM Falk, DA Yool, SR Guertin, DP AF Swetnam, T. L. Lynch, A. M. Falk, D. A. Yool, S. R. Guertin, D. P. TI Discriminating disturbance from natural variation with LiDAR in semi-arid forests in the southwestern USA SO ECOSPHERE LA English DT Article DE Anselin local Moran's I; disturbance ecology; forest structure; Getis-Ord G; LiDAR; local indicators of spatial association; topographic indices ID LOCAL SPATIAL AUTOCORRELATION; TOPOGRAPHIC WETNESS INDEX; UPPER MONTANE FORESTS; STAND-REPLACING FIRE; TREE DETECTION; UNITED-STATES; PATTERNS; ECOLOGY; SCALE; MOUNTAINS AB Discriminating amongst spatial configurations and climax size of trees in forests along varying physical gradients from time since last disturbance is a significant component of applied forest management. Understanding what has led to the existing vegetation's structure has important implications for monitoring succession and eco-hydrological interactions within the critical zone: the near-surface environment where rock, soil, air, and biota interact and regulate ecosystem services. This research demonstrates the utilities of local indicators of spatial association (LISA) to (1) quantify natural variation in forest structure as derived from aerial Light Detection and Range (LiDAR) across topographically complex landscapes at ecologically relevant scale, i.e., individual trees; (2) map previously recorded but poorly defined forest disturbances; and (3) link scalable topographic indices to observed tree size distributions. We first selected a priori undisturbed and disturbed stands scanned by aerial LiDAR that included: preservation, mechanical fuels treatment, and logging in two similar semi-arid forest ecosystems. Next, we compared two topographic indices: the topographic position index (TPI) and topographic wetness index (TWI) to climax tree height and two related LISAs: the Getis-Ord G(i) and Anselin local Moran's I. G(i) and I measure local spatial clustering, producing z-scores that represent the significance of each statistic. The tallest trees in each study area were found to be located on negative TPI and greater TWI values (valley bottom positions and higher wetness conditions, respectively). Stands of trees with positive z-scores (i.e., overstory trees with similarly tall neighbors) were most likely to be located in the a priori undisturbed areas. Disturbed locations, on the other hand, were defined by neighborhoods of negative z-scores (i.e., shorter trees with short neighbors). Future applications include locating and reconstructing historical disturbances and developing a consistent strategy for characterizing stands for forest inventory monitoring. C1 [Swetnam, T. L.] Univ Arizona, Dept Geosci, Tucson, AZ 85721 USA. [Lynch, A. M.] US Forest Serv, Rocky Mt Res Stn, Tucson, AZ 85721 USA. [Lynch, A. M.; Falk, D. A.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Falk, D. A.; Guertin, D. P.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Yool, S. R.] Univ Arizona, Sch Geog & Dev, Tucson, AZ 85721 USA. RP Swetnam, TL (reprint author), Univ Arizona, Dept Geosci, Tucson, AZ 85721 USA. EM tswetnam@email.arizona.edu FU Coronado National Forest; Santa Catalina-Jemez River Basin Critical Zone Observatory (NSF Award) [0724958]; NSF [0724958] FX We would like to thank the two anonymous reviewers for their comments which greatly improved this manuscript. This work was supported by the Coronado National Forest and the Santa Catalina-Jemez River Basin Critical Zone Observatory (NSF Award #0724958). Thanks to Craig Wilcox, Coronado N.F., for providing insight into the ecological and land use history of the Pinalenos. Jemez ALS data were provided by the Critical Zone Observatories (NSF Award #0724958), Valles Caldera Trust, and Santa Fe N.F. We are also thankful to Kay Beeley, Bandelier National Park. NR 97 TC 2 Z9 2 U1 5 U2 13 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUN PY 2015 VL 6 IS 6 AR 97 DI 10.1890/ES14-00384.1 PG 22 WC Ecology SC Environmental Sciences & Ecology GA CN4XH UT WOS:000358433300009 ER PT J AU Wen, GY Chen, C Guo, J Zhang, ZY Shang, Y Shao, HB Luo, QP Yang, J Wang, HL Wang, HC Zhang, TF Zhang, RR Cheng, GF Yu, QZ AF Wen, Guoyuan Chen, Chen Guo, Jing Zhang, Zhenyu Shang, Yu Shao, Huabin Luo, Qingping Yang, Jun Wang, Hongling Wang, Hongcai Zhang, Tengfei Zhang, Rongrong Cheng, Guofu Yu, Qingzhong TI Development of a novel thermostable Newcastle disease virus vaccine vector for expression of a heterologous gene SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID V4 STRAIN; VILLAGE CHICKENS; FOREIGN GENE; PROTEIN; CLEAVAGE; NDV; GLYCOPROTEINS; REPLICATION; PROTECTION; CHALLENGE AB Thermostable Newcastle disease virus (NDV) vaccines have been used widely to control Newcastle disease for village poultry flocks, due to their independence of cold chains for delivery and storage. To explore the potential use of thermostable NDV as a vaccine vector, an infectious clone of thermostable avirulent NDV strain TS09-C was developed using reverse genetics technology. The GFP gene, along with the self-cleaving 2A gene of foot-and-mouth disease virus and ubiquitin monomer (2AUbi), were inserted immediately upstream of the NP (nucleocapsid protein), M (matrix protein) or L (large polymerase protein) gene translation start codon in the TS09-C infectious clone. Detection of GFP expression in the recombinant virus-infected cells showed that the recombinant virus, rTS-GFP/M, with the GFP gene inserted into the M gene expressed the highest level of GFP. The rTS-GFP/M virus retained the same thermostability, growth dynamics and pathogenicity as its parental rTS09-C virus. Vaccination of specific-pathogen-free chickens with the rTS-GFP/M virus conferred complete protection against virulent NDV challenge. Taken together, the data suggested that the rTS09-C virus could be used as a vaccine vector to develop bivalent thermostable vaccines against Newcastle disease and the target avian diseases for village chickens, especially in the developing and least-developed countries. C1 [Wen, Guoyuan; Chen, Chen; Guo, Jing; Shang, Yu; Shao, Huabin; Luo, Qingping; Yang, Jun; Wang, Hongling; Wang, Hongcai; Zhang, Tengfei; Zhang, Rongrong] Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430070, PR, Peoples R China. [Wen, Guoyuan; Zhang, Zhenyu; Yu, Qingzhong] USDA, Southeast Poultry Res Lab, Agr Res Serv, Athens, GA 30605 USA. [Wen, Guoyuan] Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430070, PR, Peoples R China. [Chen, Chen; Guo, Jing; Shang, Yu; Cheng, Guofu] Huazhong Agr Univ, Coll Vet Med, Vet Pathol Lab, Wuhan 430070, PR, Peoples R China. RP Yu, QZ (reprint author), USDA, Southeast Poultry Res Lab, Agr Res Serv, Athens, GA 30605 USA. EM qingzhong.yu@ars.usda.gov FU China Agriculture Research System [CARS-42-G11]; Special Fund for Agro-scientific Research in the Public Interest [201303033]; National Natural Science Foundation of China [31000082]; US Department of Agriculture (ARS CRIS) [6612-32000-067-00D] FX The authors thank Xiuqin Xia for excellent technical assistance and Patti Miller for critical reading of the manuscript. This research was supported by China Agriculture Research System (CARS-42-G11), Special Fund for Agro-scientific Research in the Public Interest (201303033), National Natural Science Foundation of China (31000082) and US Department of Agriculture (ARS CRIS project 6612-32000-067-00D). NR 40 TC 5 Z9 6 U1 4 U2 12 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD JUN PY 2015 VL 96 BP 1219 EP 1228 DI 10.1099/vir.0.000067 PN 6 PG 10 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CN1OJ UT WOS:000358189000003 PM 25626679 ER PT J AU Wilson, WC Ruder, MG Klement, E Jasperson, DC Yadin, H Stallknecht, DE Mead, DG Howerth, E AF Wilson, William C. Ruder, Mark G. Klement, Eyal Jasperson, Dane C. Yadin, Hagai Stallknecht, David E. Mead, Daniel G. Howerth, Elizabeth TI Genetic characterization of epizootic hemorrhagic disease virus strains isolated from cattle in Israel SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID WHITE-TAILED DEER; COMPLETE NUCLEOTIDE-SEQUENCE; AFRICAN HORSE SICKNESS; BLUETONGUE VIRUS; IBARAKI VIRUS; MOLECULAR CHARACTERIZATION; SEROTYPE 7; S7 GENE; PROTEIN; ORBIVIRUS AB Epizootic hemorrhagic disease virus (EFIDV), a member of the genus Orbivirus not reported previously in Israel, was isolated from Israeli cattle during a 'bluetongue-like' disease outbreak in 2006. To ascertain the origin of this new virus, three isolates from the outbreak were fully sequenced and compared with available sequences. Whilst the L2 gene segment clustered with the Australian EHDV serotype 7 (EHDV-7) reference strain, most of the other segments were clustered with EHDV isolates of African/Middle East origin, specifically Bahrain, Nigeria and South Africa. The M6 gene had genetic relatedness to the Australian/Asian strains, but with the limited data available the significance of this relationship is unclear. Only one EHDV-7 L2 sequence was available, and as this gene encodes the Serotype-specific epitope, the relationship of these EHDV-7 L2 genes to an Australian EHDV-7 reflects the serotype association, not necessarily the origin. The genetic data indicated that the strains affecting Israel in 2006 may have been related to similar outbreaks that occurred in North Africa in the same year. This finding also supports the hypothesis that EHDV entered Israel during 2006 and was not present there before this outbreak. C1 [Wilson, William C.; Ruder, Mark G.; Jasperson, Dane C.] ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS 66502 USA. [Klement, Eyal] Hebrew Univ Jerusalem, Koret Sch Vet Med, Jerusalem, Israel. [Yadin, Hagai] Kimron Vet Inst, Div Virol, Bet Dagan, Israel. [Stallknecht, David E.; Mead, Daniel G.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Athens, GA USA. [Howerth, Elizabeth] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. RP Wilson, WC (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS 66502 USA. EM william.wilson@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service [5430-32000-006-00D]; BARD, The United States-Israel Binational Agricultural Research and Development Fund [IS-4105-08] FX The authors thank Drs N. James MacLachlan and Natasha Gaudreault for their review of this article. This work was supported by the US Department of Agriculture, Agricultural Research Service (project #5430-32000-006-00D) and BARD, The United States-Israel Binational Agricultural Research and Development Fund, research grant no. IS-4105-08. NR 45 TC 3 Z9 3 U1 0 U2 1 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD JUN PY 2015 VL 96 BP 1400 EP 1410 DI 10.1099/vir.0.000089 PN 6 PG 11 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CN1OJ UT WOS:000358189000022 PM 25701817 ER PT J AU Velmurugan, P Myung, H Govarthanan, M Yi, YJ Seo, SK Cho, KM Lovanh, N Oh, BT AF Velmurugan, Palanivel Myung, Hyun Govarthanan, Muthusamy Yi, Young-Joo Seo, Sang-Ki Cho, Kwang-Min Lovanh, Nanh Oh, Byung-Taek TI Production and characterization of Bacterial Cellulose by Leifsonia sp CBNU-EW3 Isolated from the Earthworm, Eisenia fetida SO BIOTECHNOLOGY AND BIOPROCESS ENGINEERING LA English DT Article DE bacterial cellulose; Leifsonia sp.; optimization; characterization ID MICROBIAL CELLULOSE; CULTURE-CONDITIONS; OPTIMIZATION; GENUS; STRAIN AB A total of five bacterial strains were isolated from earthworm, Eisenia fetida and examined for bacterial cellulose (BC) production in Hestrin-Schramm medium (HS). Among the five strains tested, CBNU-EW3 exhibited excellent BC production and was identified as Leifsonia sp. by 16S rDNA sequence analysis. BC production by Leifsonia sp. CBNU-EW3 was optimum at pH 5, 30 degrees C, and with glucose and yeast extract as carbon and nitrogen sources, respectively, according to 15 day-long experiments. (XRD) analysis of the dried pellicle indicated that the BC was partially crystalline type I. Fourier transform infrared spectroscopy (FT-IR) analysis showed that the obtained pellicle contained the same functional groups as typical BC. Field emission scanning electron microscopy (FESEM) images showed that the BC micro-fibril matrix consisted of a flat surface with large pore size and cellulose aggregation. C1 [Velmurugan, Palanivel; Govarthanan, Muthusamy; Yi, Young-Joo; Oh, Byung-Taek] Chonbuk Natl Univ, Coll Environm & Bioresource Sci, Adv Inst Environm & Biosci, Div Biotechnol, Iksan 570752, South Korea. [Myung, Hyun] Chonbuk Natl Univ, Coll Environm & Bioresource Sci, Dept Ecol Landscape Architecture, Iksan 570752, South Korea. [Seo, Sang-Ki] Korea Rural Community Corp, Naju 520350, South Korea. [Cho, Kwang-Min] RDA, Natl Inst Crop Sci, Iksan 570080, South Korea. [Lovanh, Nanh] USDA ARS, AWMRU, Bowling Green, KY 42104 USA. [Oh, Byung-Taek] Chonbuk Natl Univ, Coll Agr & Life Sci, Plant Med Res Ctr, Jeonju, South Korea. RP Velmurugan, P (reprint author), Chonbuk Natl Univ, Coll Environm & Bioresource Sci, Adv Inst Environm & Biosci, Div Biotechnol, Iksan 570752, South Korea. EM btoh@jbnu.ac.kr OI Velmurugan, Palanivel/0000-0001-6506-1953 FU National Research Foundation of Korea (NRF) - government (MEST) [2011-0020202] FX This research was supported by a National Research Foundation of Korea (NRF) grant funded by the government (MEST; No. 2011-0020202). NR 35 TC 0 Z9 0 U1 2 U2 9 PU KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING PI SEOUL PA KOREAN SCIENCE TECHNOLOGY CENTER, #704 YEOGSAM-DONG, KANGNAM-KU, SEOUL 135-703, SOUTH KOREA SN 1226-8372 EI 1976-3816 J9 BIOTECHNOL BIOPROC E JI Biotechnol. Bioprocess Eng. PD JUN PY 2015 VL 20 IS 3 BP 410 EP 416 DI 10.1007/s12257-014-0793-y PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CN2VG UT WOS:000358279600005 ER PT J AU Mishra, V Ellenburg, WL Al-Hamdan, OZ Bruce, J Cruise, JF AF Mishra, Vikalp Ellenburg, Walter L. Al-Hamdan, Osama Z. Bruce, Josh Cruise, James F. TI Modeling Soil Moisture Profiles in Irrigated Fields by the Principle of Maximum Entropy SO ENTROPY LA English DT Article DE maximum entropy; soil moisture; irrigation efficiency ID WATER-UPTAKE; STATISTICAL-MECHANICS; INFORMATION-THEORY; CONCEPTUAL-MODEL; STRESS; COMPLEXITY; EFFICIENCY; RETENTION; GROWTH; ROOTS AB Vertical soil moisture profiles based on the principle of maximum entropy (POME) were validated using field and model data and applied to guide an irrigation cycle over a maize field in north central Alabama (USA). The results demonstrate that a simple two-constraint entropy model under the assumption of a uniform initial soil moisture distribution can simulate most soil moisture profiles that occur in the particular soil and climate regime that prevails in the study area. The results of the irrigation simulation demonstrated that the POME model produced a very efficient irrigation strategy with minimal losses (about 1.9% of total applied water). However, the results for finely-textured (silty clay) soils were problematic in that some plant stress did develop due to insufficient applied water. Soil moisture states in these soils fell to around 31% of available moisture content, but only on the last day of the drying side of the irrigation cycle. Overall, the POME approach showed promise as a general strategy to guide irrigation in humid environments, such as the Southeastern United States. C1 [Mishra, Vikalp; Ellenburg, Walter L.; Cruise, James F.] Univ Alabama, Natl Space Sci & Technol Ctr, Ctr Earth Syst Sci, Huntsville, AL 35805 USA. [Al-Hamdan, Osama Z.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Bruce, Josh] Univ Alabama, Dept Civil & Environm Engn, Huntsville, AL 35899 USA. RP Cruise, JF (reprint author), Univ Alabama, Natl Space Sci & Technol Ctr, Ctr Earth Syst Sci, 320 Sparkman Dr, Huntsville, AL 35805 USA. EM vikalp.mishra@nsstc.uah.edu; lee.ellenburg@uah.edu; Osama.Al-Hamdan@ars.usda.gov; jgb0010@alumni.uah.edu; james.cruise@nsstc.uah.edu FU joint NSF/USDA Project "F/USDA/NIFA Type 2-Collaborative Research-Migration of Agricultural Production Back to the Southeast" FX The authors would like to express their sincere gratitude to the anonymous reviewers for their constructive comments towards the improvment of this manuspricpt. This research was supported under the joint NSF/USDA Project "F/USDA/NIFA Type 2-Collaborative Research-Migration of Agricultural Production Back to the Southeast". The support of the project PI, Richard McNider, is appreciated. NR 61 TC 2 Z9 2 U1 0 U2 2 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1099-4300 J9 ENTROPY-SWITZ JI Entropy PD JUN PY 2015 VL 17 IS 6 BP 4454 EP 4484 DI 10.3390/e17064454 PG 31 WC Physics, Multidisciplinary SC Physics GA CM6MO UT WOS:000357803000043 ER PT J AU Wang, DG Wang, YZ Xiao, FG Guo, WY Zhang, YQ Wang, AP Liu, YH AF Wang, Deguo Wang, Yongzhen Xiao, Fugang Guo, Weiyun Zhang, Yongqing Wang, Aiping Liu, Yanhong TI A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria SO MOLECULES LA English DT Article DE real-time loop-mediated isothermal amplification (LAMP); specificity; sensitivity; pathogenic bacteria ID MEDIATED ISOTHERMAL AMPLIFICATION; RAPID DETECTION; O157 AB Molecular detection of bacterial pathogens based on LAMP methods is a faster and simpler approach than conventional culture methods. Although different LAMP-based methods for pathogenic bacterial detection are available, a systematic comparison of these different LAMP assays has not been performed. In this paper, we compared 12 in-house real-time LAMP assays with a commercialized kit (Isothermal Master Mix) for the detection of Listeria monocytogenes, Salmonella spp, Staphylococcus aureus, Escherichia coli O157, E. coli O26, E. coli O45, E. coli O103, E. coli O111, E. coli O121, E. coli O145 and Streptococcus agalactiae. False-positive results were observed in all 12 in-house real-time LAMP assays, while all the negative controls of Isothermal Master Mix remained negative after amplification. The detection limit of Isothermal Master Mix for Listeria monocytogenes, Salmonella spp, Staphylococcus aureus, Escherichia coli O157, E. coli O26, E. coli O45, E. coli O103, E. coli O111, E. coli O121 and Streptococcus agalactiae was 1 pg, whereas the sensitivity of the commercialized kit for E. coli O145 was 100 pg. In conclusion, the 12 in-house real-time LAMP assays were impractical to use, while the commercialized kit Isothermal Master Mix was useful for the detection of most bacterial pathogens. C1 [Wang, Deguo; Xiao, Fugang; Guo, Weiyun; Zhang, Yongqing] Xuchang Univ, Food & Bioengn Coll, Henan Postdoctoral Res Base, Xuchang 461000, Henan, Peoples R China. [Wang, Yongzhen] Xuchang Univ, Publ Lab Ctr, Xuchang 461000, Henan, Peoples R China. [Wang, Aiping] Zhengzhou Univ, Life Sci Coll, Zhengzhou 450000, Henan, Peoples R China. [Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Liu, YH (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM wangdg666@126.com; wangyzhxuch@sina.com; xfug@163.com; gwy2002@126.com; zyq336@163.com; pingaw@126.com; yanhong.liu@ars.usda.gov FU China Scholarship Council; Natural Science Foundation of China [31172331]; NSFC-Henan Talent Training Joint Foundation [U1204330]; Project for Scientific Research AMP; Innovation Team of Henan Universities [15IRTSTHN016]; Foundation of Henan Province for Excellent Young Teachers of Colleges and Universities [2012GGJS-172]; Xuchang University for Outstanding Young Backbone Teachers; Xuchang University FX The authors would like to acknowledge the China Scholarship Council. This work was supported by Natural Science Foundation of China (31172331), NSFC-Henan Talent Training Joint Foundation (U1204330), Project for Scientific Research & Innovation Team of Henan Universities (15IRTSTHN016), Foundation of Henan Province for Excellent Young Teachers of Colleges and Universities (2012GGJS-172), Project of Xuchang University for Outstanding Young Backbone Teachers, and Training Project for Outstanding Youth Backbone Personnel of Xuchang University. NR 15 TC 3 Z9 5 U1 3 U2 24 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD JUN PY 2015 VL 20 IS 6 BP 9487 EP 9495 DI 10.3390/molecules20069487 PG 9 WC Chemistry, Organic SC Chemistry GA CM8ZE UT WOS:000357992700001 PM 26020698 ER PT J AU Nguyen, MM Al-Abdul-Wahid, MS Fontenot, KR Graves, EE Chang, SC Condon, BD Grimm, CC Lorigan, GA AF Nguyen, Monique M. Al-Abdul-Wahid, M. Sameer Fontenot, Krystal R. Graves, Elena E. Chang, SeChin Condon, Brian D. Grimm, Casey C. Lorigan, Gary A. TI Understanding the Mechanism of Action of Triazine-Phosphonate Derivatives as Flame Retardants for Cotton Fabric SO MOLECULES LA English DT Article DE triazine-phosphonate; thermal degradation; mechanism; ATR-IR; TGA-FTIR; P-31 solid state NMR; phosphoric acid ID BOND-DISSOCIATION ENERGIES; THERMAL-DECOMPOSITION; MELAMINE POLYPHOSPHATE; FIRE BEHAVIOR; CELLULOSE; DEGRADATION; GAS; ACTIVATION; PHOSPHATE; NITROGEN AB Countless hours of research and studies on triazine, phosphonate, and their combination have provided insightful information into their flame retardant properties on polymeric systems. However, a limited number of studies shed light on the mechanism of flame retardancy of their combination on cotton fabrics. The purpose of this research is to gain an understanding of the thermal degradation process of two triazine-phosphonate derivatives on cotton fabric. The investigation included the preparation of diethyl 4,6-dichloro-1,3,5-triazin-2-ylphosphonate (TPN1) and dimethyl (4,6-dichloro-1,3,5-triazin-2-yloxy) methyl phosphonate (TPN3), their application on fabric materials, and the studies of their thermal degradation mechanism. The studies examined chemical components in both solid and gas phases by using attenuated total reflection infrared (ATR-IR) spectroscopy, thermogravimetric analysis coupled with Fourier transform infrared (TGA-FTIR) spectroscopy, and P-31 solid state nuclear magnetic resonance (P-31 solid state NMR), in addition to the computational studies of bond dissociation energy (BDE). Despite a few differences in their decomposition, TPN1 and TPN3 produce one common major product that is believed to help reduce the flammability of the fabric. C1 [Nguyen, Monique M.; Fontenot, Krystal R.; Graves, Elena E.; Chang, SeChin; Condon, Brian D.; Grimm, Casey C.] USDA, Cotton Chem & Utilizat Res, New Orleans, LA 70124 USA. [Al-Abdul-Wahid, M. Sameer; Lorigan, Gary A.] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. RP Nguyen, MM (reprint author), USDA, Cotton Chem & Utilizat Res, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM monique.nguyen@ars.usda.gov; s.wahid@miamioh.edu; krystal.fontenot@ars.usda.gov; elena.graves@ars.usda.gov; sechin.chang@ars.usda.gov; brian.condon@ars.usda.gov; casey.grimm@ars.usda.gov; lorigag@miamioh.edu FU U.S. Department of Agriculture FX This project was supported by the U.S. Department of Agriculture. NR 74 TC 1 Z9 1 U1 6 U2 40 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD JUN PY 2015 VL 20 IS 6 BP 11236 EP 11256 DI 10.3390/molecules200611236 PG 21 WC Chemistry, Organic SC Chemistry GA CM8ZE UT WOS:000357992700107 PM 26096432 ER PT J AU Arias, RS Martinez-Castillo, J Sobolev, VS Blancarte-Jasso, NH Simpson, SA Ballard, LL Duke, MV Liu, XF Irish, BM Scheffler, BE AF Arias, Renee S. Martinez-Castillo, Jaime Sobolev, Victor S. Blancarte-Jasso, Nasib H. Simpson, Sheron A. Ballard, Linda L. Duke, Mary V. Liu, Xiaofen F. Irish, Brian M. Scheffler, Brian E. TI Development of a Large Set of Microsatellite Markers in Zapote Mamey (Pouteria sapota (Jacq.) HE Moore & Stearn) and Their Potential Use in the Study of the Species SO MOLECULES LA English DT Article DE blast analysis; founder effect; genetic diversity; germplasm; domestication; Mexico; SSR markers; genetic structure ID SSR MARKERS; DOMESTICATION; POPULATIONS; FRUITS; SEQUENCES; REGION; MEXICO; LOCI; L. AB Pouteria sapota is known for its edible fruits that contain unique carotenoids, as well as for its fungitoxic, anti-inflammatory and anti-oxidant activity. However, its genetics is mostly unknown, including aspects about its genetic diversity and domestication process. We did high-throughput sequencing of microsatellite-enriched libraries of P. sapota, generated 5223 contig DNA sequences, 1.8 Mbp, developed 368 microsatellites markers and tested them on 29 individuals from 10 populations (seven wild, three cultivated) from Mexico, its putative domestication center. Gene ontology BLAST analysis of the DNA sequences containing microsatellites showed potential association to physiological functions. Genetic diversity was slightly higher in cultivated than in the wild gene pool (H-E = 0.41 and H-E = 0.35, respectively), although modified Garza-Williamson Index and Bottleneck software showed evidence for a reduction in genetic diversity for the cultivated one. Neighbor Joining, 3D Principal Coordinates Analysis and assignment tests grouped most individuals according to their geographic origin but no clear separation was observed between wild or cultivated gene pools due to, perhaps, the existence of several admixed populations. The developed microsatellites have a great potential in genetic population and domestication studies of P. sapota but additional sampling will be necessary to better understand how the domestication process has impacted the genetic diversity of this fruit crop. C1 [Arias, Renee S.; Sobolev, Victor S.] USDA ARS Natl Peanut Res Lab, Dawson, GA 39842 USA. [Martinez-Castillo, Jaime; Blancarte-Jasso, Nasib H.] Ctr Invest Cient Yucatan A C, Unidad Recursos Nat, CP-97200 Colonia Chuburn Hid, Merida, Mexico. [Simpson, Sheron A.; Ballard, Linda L.; Duke, Mary V.; Liu, Xiaofen F.; Scheffler, Brian E.] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Irish, Brian M.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. RP Martinez-Castillo, J (reprint author), Ctr Invest Cient Yucatan A C, Unidad Recursos Nat, Calle 43 130, CP-97200 Colonia Chuburn Hid, Merida, Mexico. EM renee.arias@ars.usda.gov; jmartinez@cicy.mx; victor.sobolev@ars.usda.gov; hiram.blancarte@cicy.mx; sheron.simpson@ars.usda.gov; linda.ballard@ars.usda.gov; mary.duke@ars.usda.gov; fanny.liu@ars.usda.gov; brian.irish@ars.usda.gov; brian.scheffler@ars.usda.gov OI Scheffler, Brian/0000-0003-1968-8952 FU USDA-ARS [6402-21310-004-00, 6635-21000-051-00, 6604-13210-005-02] FX We thank Valerie A. Orner and Matilde M. Ortiz Garcia for their technical assistance and Paulino Sima for his work assistance. This work was supported by USDA-ARS project numbers 6402-21310-004-00, 6635-21000-051-00 and 6604-13210-005-02. NR 62 TC 1 Z9 1 U1 1 U2 6 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD JUN PY 2015 VL 20 IS 6 BP 11400 EP 11417 DI 10.3390/molecules200611400 PG 18 WC Chemistry, Organic SC Chemistry GA CM8ZE UT WOS:000357992700116 PM 26111173 ER PT J AU de Cock, AWAM Lodhi, AM Rintoul, TL Bala, K Robideau, GP Abad, ZG Coffey, MD Shahzad, S Levesque, CA AF de Cock, A. W. A. M. Lodhi, A. M. Rintoul, T. L. Bala, K. Robideau, G. P. Abad, Z. Gloria Coffey, M. D. Shahzad, S. Levesque, C. A. TI Phytopythium: molecular phylogeny and systematics SO PERSOONIA LA English DT Article DE COI; LSU; Oomycetes; Oomycota; Peronosporales; Phytopythium; Pythiales; SSU ID INTERNAL TRANSCRIBED SPACER; PYTHIUM; PHYTOPHTHORA; TAXONOMY; EVOLUTION; FAMILY AB The genus Phytopythium (Peronosporales) has been described, but a complete circumscription has not yet been presented. In the present paper we provide molecular-based evidence that members of Pythium clade K as described by Levesque & de Cock (2004) belong to Phytopythium. Maximum likelihood and Bayesian phylogenetic analysis of the nuclear ribosomal DNA (LSU and SSU) and mitochondrial DNA cytochrome oxidase subunit 1 (COI) as well as statistical analyses of pairwise distances strongly support the status of Phytopythium as a separate phylogenetic entity. Phytopythium is morphologically intermediate between the genera Phytophthora and Pythium. It is unique in having papillate, internally proliferating sporangia and cylindrical or lobate antheridia. The formal transfer of clade K species to Phytopythium and a comparison with morphologically similar species of the genera Pythium and Phytophthora is presented. A new species is described, Phytopythium mirpurense. C1 [de Cock, A. W. A. M.] CBS KNAW Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands. [Lodhi, A. M.] Sindh Agr Univ, Dept Plant Pathol, Tandojam, Pakistan. [Rintoul, T. L.; Bala, K.; Robideau, G. P.; Levesque, C. A.] Agr & Agri Food Canada, Ottawa, ON K1A 0C6, Canada. [Abad, Z. Gloria] USDA APHIS PPQ, Ctr Plant Hlth Sci & Technol, Beltsville, MD 20705 USA. [Coffey, M. D.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Shahzad, S.] Univ Karachi, Dept Agr, Karachi, Pakistan. RP Levesque, CA (reprint author), Agr & Agri Food Canada, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada. EM Andre.Levesque@agr.gc.ca FU Genome Canada through the Ontario Genomics Institute; NSERC FX We thank Nicole Desaulniers for assistance in maintaining Phytopythium cultures, Rafik Assabgui and Julie Chapados from Agriculture and Agri-Food Canada, Ottawa for sequencing these strains. Strains were received from Anne Dorrance from The Ohio State University, Food, Agricultural, and Environmental Sciences, Plant Pathology, Columbus, OH, USA. We thank Marjan Vermaas for composing the photo plates. This research was supported through funding to the Consortium for the Barcode of Life Network (CBOL) from Genome Canada (through the Ontario Genomics Institute), NSERC and other sponsors listed at http://www.BOLNET.ca. NR 34 TC 16 Z9 18 U1 2 U2 15 PU RIJKSHERBARIUM PI LEIDEN PA PO BOX 9514, 2300 RA LEIDEN, NETHERLANDS SN 0031-5850 J9 PERSOONIA JI Persoonia PD JUN PY 2015 VL 34 BP 25 EP 39 DI 10.3767/003158515X685382 PG 15 WC Mycology SC Mycology GA CN1MF UT WOS:000358183400003 PM 26240443 ER PT J AU AghaKouchak, A Farahmand, A Melton, FS Teixeira, J Anderson, MC Wardlow, BD Hain, CR AF AghaKouchak, A. Farahmand, A. Melton, F. S. Teixeira, J. Anderson, M. C. Wardlow, B. D. Hain, C. R. TI Remote sensing of drought: Progress, challenges and opportunities SO REVIEWS OF GEOPHYSICS LA English DT Review DE drought; remote sensing ID LAND-SURFACE TEMPERATURE; SNOW WATER EQUIVALENT; MONITORING METEOROLOGICAL DROUGHT; POLARIZATION DIFFERENCE INDEX; VEGETATION CONDITION INDEX; PASSIVE MICROWAVE SENSORS; NINO SOUTHERN-OSCILLATION; EUCALYPTUS-MACULATA HOOK; AMAZON RAIN-FORESTS; SOIL-MOISTURE INDEX AB This review surveys current and emerging drought monitoring approaches using satellite remote sensing observations from climatological and ecosystem perspectives. We argue that satellite observations not currently used for operational drought monitoring, such as near-surface air relative humidity data from the Atmospheric Infrared Sounder mission, provide opportunities to improve early drought warning. Current and future satellite missions offer opportunities to develop composite and multi-indicator drought models. While there are immense opportunities, there are major challenges including data continuity, unquantified uncertainty, sensor changes, and community acceptability. One of the major limitations of many of the currently available satellite observations is their short length of record. A number of relevant satellite missions and sensors (e.g., the Gravity Recovery and Climate Experiment) provide only a decade of data, which may not be sufficient to study droughts from a climate perspective. However, they still provide valuable information about relevant hydrologic and ecological processes linked to this natural hazard. Therefore, there is a need for models and algorithms that combine multiple data sets and/or assimilate satellite observations into model simulations to generate long-term climate data records. Finally, the study identifies a major gap in indicators for describing drought impacts on the carbon and nitrogen cycle, which are fundamental to assessing drought impacts on ecosystems. C1 [AghaKouchak, A.; Farahmand, A.] Univ Calif Irvine, Ctr Hydrometeorol & Remote Sensing, Irvine, CA 92697 USA. [Melton, F. S.] NASA Ames Res Ctr Cooperat Res Earth Sci & Techno, Moffett Field, CA USA. [Teixeira, J.] CALTECH, NASA Jet Prop Lab, Pasadena, CA 91125 USA. [Anderson, M. C.] ARS, USDA, Beltsville, MD USA. [Wardlow, B. D.] Univ Nebraska, Sch Nat Resources, Lincoln, NE USA. [Hain, C. R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP AghaKouchak, A (reprint author), Univ Calif Irvine, Ctr Hydrometeorol & Remote Sensing, Irvine, CA 92697 USA. EM amir.a@uci.edu RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU National Aeronautics and Space Administration (NASA) [NNX15AC27G] FX This study is supported by the National Aeronautics and Space Administration (NASA) award NNX15AC27G. The input data sets and final outputs presented in this paper are all freely available through the Global Integrated Drought Monitoring and Prediction System (GIDMaPS; http://drought.eng.uci.edu/), and the NOAA/NIDIS Global Vegetation Health data (http://www.star.nesdis.noaa.gov/smcd/emb/vci/VH/). NR 329 TC 27 Z9 28 U1 26 U2 106 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 8755-1209 EI 1944-9208 J9 REV GEOPHYS JI Rev. Geophys. PD JUN PY 2015 VL 53 IS 2 BP 452 EP 480 DI 10.1002/2014RG000456 PG 29 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA CN3JO UT WOS:000358322200008 ER PT J AU Miesel, JR Hockaday, WC Kolka, RK Townsend, PA AF Miesel, Jessica R. Hockaday, William C. Kolka, Randall K. Townsend, Philip A. TI Soil organic matter composition and quality across fire severity gradients in coniferous and deciduous forests of the southern boreal region SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE wildfire; Pagami Creek fire; forest; soil organic matter; carbon; pyrogenic carbon ID C-13 NMR-SPECTRA; GLOBAL WILDLAND FIRE; BLACK CARBON; DETERMINING QUANTITATION; NORTHERN MINNESOTA; PRESCRIBED FIRE; PINE FOREST; CHARCOAL; NITROGEN; WILDFIRE AB Recent patterns of prolonged regional drought in southern boreal forests of the Great Lakes region, USA, suggest that the ecological effects of disturbance by wildfire may become increasingly severe. Losses of forest soil organic matter (SOM) during fire can limit soil nutrient availability and forest regeneration. These processes are also influenced by the composition of postfire SOM. We sampled the forest floor layer (i.e., full organic horizon) and 0-10cm mineral soil from stands dominated by coniferous (Pinus banksiana Lamb.) or deciduous (Populus tremuloides Michx.) species 1-2months after the 2011 Pagami Creek wildfire in northern Minnesota. We used solid-state C-13 NMR to characterize SOM composition across a gradient of fire severity in both forest cover types. SOM composition was affected by fire, even when no statistically significant losses of total C stocks were evident. The most pronounced differences in SOM composition between burned and unburned reference areas occurred in the forest floor for both cover types. Carbohydrate stocks in forest floor and mineral horizons decreased with severity level in both cover types, whereas pyrogenic C stocks increased with severity in the coniferous forest floor and decreased in only the highest severity level in the deciduous forest floor. Loss of carbohydrate and lignin pools contributed to a decreased SOM stability index and increased decomposition index. Our results suggest that increases in fire severity expected to occur under future climate scenarios may lead to changes in SOM composition and dynamics with consequences for postfire forest recovery and C uptake. C1 [Miesel, Jessica R.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Hockaday, William C.] Baylor Univ, Dept Geol, Waco, TX 76798 USA. [Kolka, Randall K.] USDA Forest Serv, Northern Res Stn, Grand Rapids, MN USA. [Townsend, Philip A.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI USA. RP Miesel, JR (reprint author), Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. EM mieselje@msu.edu RI Townsend, Philip/B-5741-2008; OI Townsend, Philip/0000-0001-7003-8774; Hockaday, William/0000-0002-0501-0393; Miesel, Jessica/0000-0001-7446-464X FU NSF RAPID [1201484]; NSF EAR-IF [1132124]; Michigan State University FX Data used for the results presented are available from the corresponding author upon request. Funding for this project was provided by NSF RAPID award 1201484 to Phil Townsend, Randall Kolka, and others, by an NSF EAR-IF 1132124 to Bill Hockaday, and by Michigan State University. We thank Sue Leitz for providing the fire location map, Charles Perry for providing prefire FIA data, the Townsend Lab and Superior National Forest personnel for field assistance, Nate Aspelin for lab assistance, Stephanie Grand for helpful discussion of the data, and Bernardo Maestrini and Lucas Michelotti for comments on the Discussion. All sampling was performed in compliance with agency guidelines and permitting requirements for research in federally designated wilderness areas. We thank two anonymous reviewers for their helpful comments on an earlier version of this manuscript. NR 80 TC 2 Z9 2 U1 10 U2 50 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUN PY 2015 VL 120 IS 6 BP 1124 EP 1141 DI 10.1002/2015JG002959 PG 18 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CM8LM UT WOS:000357952400010 ER PT J AU Cuezzo, F Calcaterra, LA Chifflet, L Follett, P AF Cuezzo, F. Calcaterra, L. A. Chifflet, L. Follett, P. TI Wasmannia Forel (Hymenoptera: Formicidae: Myrmicinae) in Argentina: Systematics and Distribution SO SOCIOBIOLOGY LA English DT Article DE Wasmannia longiseta n. sp.; little fire ant; biogeography; taxonomy; phylogeny ID FIRE ANT; PHYLOGENETIC ANALYSIS; CONTINUOUS CHARACTERS; MAYR HYMENOPTERA; AUROPUNCTATA; POPULATIONS; GYNANDROMORPHISM; REPRODUCTION; INVASION; SEX AB The ant genus Wasmannia is endemic to the Neotropics, with 10 species occurring within the presumptive native range for the genus from Mexico to Argentina. Only the little fire ant, Wasmannia auropunctata is widely distributed being present from central-eastern Argentina to Bermuda, and has become infamous due to its recent worldwide expansion and status as an invasive pest. The objective of this work was to study the systematics and distribution of Wasmannia in its southern limit of distribution in Argentina. Out of the three species reported so far for Argentina, only W. auropunctata was found to be widely distributed, but abundant only in disturbed habitats mostly in the Northeast. Herein, the distribution of Wasmannia auropunctata is extended and its queen and male castes are redescribed, along with descriptions of gynandromorphs (specimens with left side of the head similar to a male and right side similar to a queen). Wasmannia sulcaticeps and W. williamsoni are much less common and widespread. W. sulcaticepsis mostly found in mountain forests in northwestern Argentina, whereas W. williamsoni is only found in shrublands and grasslands in central eastern Argentina, and most frequently in mountain grasslands. Both species overlap with W. auropunctata, which is more common in the lowlands, between approximately 400 and 1000 m elevation. The queen of W. williamsoni is described and queen and male of W. sulcaticeps are redescribed. A new species, Wasmannia longiseta n. sp. Cuezzo and Calcaterra, recently found in northeastern Argentina, is described based on worker morphology. Wasmannia rochai is recorded for the first time in Misiones, extending its distribution range from Sao Paulo (Brazil) to Misiones in northeastern Argentina. A key to the worker caste is provided. A cladistic analysis based on discrete and continuous morphological characters is presented as a first attempt to clarify the phylogenetic relationships between the known species of Wasmannia. C1 [Cuezzo, F.] Consejo Nacl Invest Cient & Tecn, Fac Ciencias Nat & IML, Inst Super Entomol Dr A Willink, San Miguel De Tucuman, Argentina. [Calcaterra, L. A.] Fdn Estudio Especies Invas, CONICET, Buenos Aires, DF, Argentina. [Chifflet, L.] Univ Buenos Aires, CONICET, Buenos Aires, DF, Argentina. [Follett, P.] USDA ARS, Pacific Basin Agr Res Ctr, Hilo, HI USA. RP Cuezzo, F (reprint author), Fac Ciencias Nat & IML, Inst Super Entomol Dr A Willink, Miguel Lillo 205,T4000JFE, San Miguel De Tucuman, Tucuman, Argentina. EM cuezzof@yahoo.com.ar FU USDA-ARS-Pacific Basin Agricultural Research Center; FuEDEI; National Scientific and Technical Research Council (CONICET, Argentina); Universidad Nacional de Tucuman [26/G413 CIUNT] FX We thank to the students Sonia Cabrera and Leonardo Ramirez for their field assistance, and Santiago Santoandre, Carolina Ramos, Francisco Sola, Julieta Fillol, and Adriana Aranda Rickert, who provided exemplars of Wasmannia species included in this study. We also thank three anonymous reviewers for the critical view of this article. This research was partly funded by USDA-ARS-Pacific Basin Agricultural Research Center, USDA-ARS-South American Biological Control Laboratory (now FuEDEI); the National Scientific and Technical Research Council (CONICET, Argentina) and the project 26/G413 CIUNT, Universidad Nacional de Tucuman. NR 50 TC 2 Z9 2 U1 3 U2 9 PU UNIV ESTADUAL FEIRA SANTANA PI FEIRA DE SANTANA PA AV TRANSORDESTINA S N NOVO HORIZONTE, FEIRA DE SANTANA, BAHAI CEP44036-900, BRAZIL SN 0361-6525 J9 SOCIOBIOLOGY JI Sociobiology PD JUN PY 2015 VL 62 IS 2 BP 246 EP 265 PG 20 WC Entomology SC Entomology GA CM4RW UT WOS:000357673400010 ER PT J AU Asao, S Ryan, MG AF Asao, Shinichi Ryan, Michael G. TI Carbohydrate regulation of photosynthesis and respiration from branch girdling in four species of wet tropical rain forest trees SO TREE PHYSIOLOGY LA English DT Article DE non-structural carbohydrates; NSC; phloem; sink regulation ID GIBBERELLIC-ACID APPLICATION; PHLOEM LOADING STRATEGIES; NET CO2 ASSIMILATION; DOUGLAS-FIR TREES; SOIL RESPIRATION; SINK-REGULATION; WATER RELATIONS; GAS-EXCHANGE; PLANT-GROWTH; LEAF PHOTOSYNTHESIS AB How trees sense source-sink carbon balance remains unclear. One potential mechanism is a feedback from non-structural carbohydrates regulating photosynthesis and removing excess as waste respiration when the balance of photosynthesis against growth and metabolic activity changes. We tested this carbohydrate regulation of photosynthesis and respiration using branch girdling in four tree species in a wet tropical rainforest in Costa Rica. Because girdling severs phloem to stop carbohydrate export while leaving xylem intact to allow photosynthesis, we expected carbohydrates to accumulate in leaves to simulate a carbon imbalance. We varied girdling intensity by removing phloem in increments of one-quarter of the circumference (zero, one-quarter, half, three-quarters, full) and surrounded a target branch with fully girdled ones to create a gradient in leaf carbohydrate content. Light saturated photosynthesis rate was measured in situ, and foliar respiration rate and leaf carbohydrate content were measured after destructive harvest at the end of the treatment. Girdling intensity created no consistent or strong responses in leaf carbohydrates. Glucose and fructose slightly increased in all species by 3.4% per one-quarter girdle, total carbon content and leaf mass per area increased only in one species by 5.4 and 5.5% per one-quarter girdle, and starch did not change. Only full girdling lowered photosynthesis in three of four species by 59-69%, but the decrease in photosynthesis was unrelated to the increase in glucose and fructose content. Girdling did not affect respiration. The results suggest that leaf carbohydrate content remains relatively constant under carbon imbalance, and any changes are unlikely to regulate photosynthesis or respiration. Because girdling also stops the export of hormones and reactive oxygen species, girdling may induce physiological changes unrelated to carbohydrate accumulation and may not be an effective method to study carbohydrate feedback in leaves. In three species, removal of three-quarters of phloem area did not cause leaf carbohydrates to accumulate nor did it change photosynthesis or respiration, suggesting that phloem transport is flexible and transport rate per unit phloem can rapidly increase under an increase in carbohydrate supply relative to phloem area. Leaf carbohydrate content thus may be decoupled from whole plant carbon balance by phloem transport in some species, and carbohydrate regulation of photosynthesis and respiration may not be as common in trees as previous girdling studies suggest. Further studies in carbohydrate regulation should avoid using girdling as girdling can decrease photosynthesis through unintended means without the tested mechanisms of accumulating leaf carbohydrates. C1 [Asao, Shinichi] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Asao, Shinichi; Ryan, Michael G.] Colorado State Univ, Nat Resources Ecol Lab, Ft Collins, CO 80523 USA. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Asao, S (reprint author), Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. EM shinchi.asao@colostate.edu RI Ryan, Michael/A-9805-2008; Asao, Shinichi/R-9514-2016 OI Ryan, Michael/0000-0002-2500-6738; Asao, Shinichi/0000-0002-0334-5464 FU National Science Foundation [EF-0236502, DEB-0703561]; Australia's Commonwealth Scientific and Industrial Research Organization (CSIRO) FX This research was supported by National Science Foundation grants EF-0236502 and DEB-0703561. M.G.R. was partially supported by Australia's Commonwealth Scientific and Industrial Research Organization (CSIRO) McMaster Fellowship. NR 109 TC 1 Z9 2 U1 6 U2 60 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD JUN PY 2015 VL 35 IS 6 BP 608 EP 620 DI 10.1093/treephys/tpv025 PG 13 WC Forestry SC Forestry GA CM7RK UT WOS:000357893400004 PM 25870320 ER PT J AU Swift, SM Seal, BS Garrish, JK Oakley, BB Hiett, K Yeh, HY Woolsey, R Schegg, KM Line, JE Donovan, DM AF Swift, Steven M. Seal, Bruce S. Garrish, Johnna K. Oakley, Brian B. Hiett, Kelli Yeh, Hung-Yueh Woolsey, Rebekah Schegg, Kathleen M. Line, John Eric Donovan, David M. TI A Thermophilic Phage Endolysin Fusion to a Clostridium perfringens-Specific Cell Wall Binding Domain Creates an Anti-Clostridium Antimicrobial with Improved Thermostability SO VIRUSES-BASEL LA English DT Article ID INTESTINAL BACTERIAL COMMUNITY; TANDEM MASS-SPECTROMETRY; EGG-WHITE LYSOZYME; STAPHYLOCOCCUS-AUREUS; STATISTICAL-MODEL; GROWTH PROMOTERS; BROILER-CHICKENS; EXPRESSION; SUSCEPTIBILITY; ALTERNATIVES AB Clostridium perfringens is the third leading cause of human foodborne bacterial disease and is the presumptive etiologic agent of necrotic enteritis among chickens. Treatment of poultry with antibiotics is becoming less acceptable. Endolysin enzymes are potential replacements for antibiotics. Many enzymes are added to animal feed during production and are subjected to high-heat stress during feed processing. To produce a thermostabile endolysin for treating poultry, an E. coli codon-optimized gene was synthesized that fused the N-acetylmuramoyl-l-alanine amidase domain from the endolysin of the thermophilic bacteriophage phi GVE2 to the cell-wall binding domain (CWB) from the endolysin of the C. perfringens-specific bacteriophage phi CP26F. The resulting protein, PlyGVE2CpCWB, lysed C. perfringens in liquid and solid cultures. PlyGVE2CpCWB was most active at pH 8, had peak activity at 10 mM NaCl, 40% activity at 150 mM NaCl and was still 16% active at 600 mM NaCl. The protein was able to withstand temperatures up to 50 degrees C and still lyse C. perfringens. Herein, we report the construction and characterization of a thermostable chimeric endolysin that could potentially be utilized as a feed additive to control the bacterium during poultry production. C1 [Swift, Steven M.; Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA,BARC East, Beltsville, MD 20705 USA. [Seal, Bruce S.; Garrish, Johnna K.; Oakley, Brian B.; Hiett, Kelli; Yeh, Hung-Yueh; Line, John Eric] ARS, Poultry Microbiol Safety & Proc Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. [Woolsey, Rebekah; Schegg, Kathleen M.] Univ Nevada, Nevada Prote Ctr, Sch Med, Reno, NV 89557 USA. RP Donovan, DM (reprint author), ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA,BARC East, 10300 Baltimore Ave,Bldg 230, Beltsville, MD 20705 USA. EM steven.swift@ars.usda.gov; sealb@onid.oregonstate.edu; johnna.garrish@ars.usda.gov; boakley@westernu.edu; keli.hiett@ars.usda.gov; hungyueh.yeh@ars.usda.gov; rebekahw@unr.edu; schegg@unr.edu; eric.line@ars.usda.gov; David.Donovan@ars.usda.gov OI Swift, Steven/0000-0002-3840-4401 FU U.S. Department of Agriculture, Agricultural Research Service [6612-32000-060, 8042-31000-103]; National Center for Research Resources from the National Institutes of Health [5P20RR016464-11]; National Institute of General Medical Sciences from the National Institutes of Health [8 P20 GM103440-11] FX Support for the research was provided by the U.S. Department of Agriculture, Agricultural Research Service (ARS CRIS project #6612-32000-060 and ARS CRIS project #8042-31000-103). The authors acknowledge primary amino acid sequencing and mass spectrometry analyses of the recombinant protein by the Nevada Proteomics Center, which operates under the auspices of grants from the National Center for Research Resources (5P20RR016464-11) and the National Institute of General Medical Sciences (8 P20 GM103440-11) from the National Institutes of Health. Supplementary information is available for this manuscript. NOTE: Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the ARS, USDA. NR 55 TC 6 Z9 6 U1 1 U2 14 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4915 J9 VIRUSES-BASEL JI Viruses-Basel PD JUN PY 2015 VL 7 IS 6 BP 3019 EP 3034 DI 10.3390/v7062758 PG 16 WC Virology SC Virology GA CM6LC UT WOS:000357799000014 PM 26075507 ER PT J AU Sanchez-Cohen, I Diaz-Padilla, G Velasquez-Valle, M Slack, DC Heilman, P Pedroza-Sandoval, A AF Sanchez-Cohen, I. Diaz-Padilla, G. Velasquez-Valle, M. Slack, D. C. Heilman, P. Pedroza-Sandoval, A. TI A decision support system for rainfed agricultural areas of Mexico SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Water balance; Risk; Model; Knowledge base ID CURVE NUMBER; MODEL AB Rural inhabitants of arid lands constantly face a lack of sufficient water to fulfill their agricultural and household needs. In this situation they have to take quick and precise decisions about how to cope with the situation. Moreover, there is not readily available technical information to support their decisions regarding the course of action they should follow to handle the agro-climatic risk. In this paper a computer model (soil water balance model) is described to assess the impact on crops yields of rainfall shortages in dry lands in Mexico. The model is linked to a knowledge based database where a farmer may find readily available information to support cropping decisions. The knowledge base activates when the computed average crop yield is less than the 50% of the expected crop yield. The knowledge base provides information on risk, potential crops, and the geographical location (counties) where the crop may succeed. Also, it provides a technology to increase water productivity under limited availability situations. Further, the model can evaluate the impact of a climate change scenario (IPCC B2). Other inputs to the model being equal, the user may shift the model to run the climate change scenario and to compare the outputs of the model to assess the climate change impact on future crops yields. (C) 2015 Elsevier B.V. All rights reserved. C1 [Sanchez-Cohen, I.; Velasquez-Valle, M.] Agr & Anim Husb Res Mexico, Natl Inst Forestry, Natl Ctr Disciplinary Res Soil Water Plant Atmosp, Gomez Palacio, Durango, Mexico. [Diaz-Padilla, G.] Reg Ctr Ctr Gulf Mexico, Natl Inst Forestry Agr & Anim Husb Res Mexico, Cotaxtla, Veracruz, Mexico. [Slack, D. C.] Univ Arizona, Agr & Bio Syst Engn, Tucson, AZ USA. [Heilman, P.] ARS, USDA, Tucson, AZ 85719 USA. [Pedroza-Sandoval, A.] Autonomous Univ Chapingo, Reg Unit Arid Lands, Bermejillo, Durango, Mexico. [Pedroza-Sandoval, A.] Dom Conocido, Bermejillo, Durango, Mexico. RP Sanchez-Cohen, I (reprint author), Agr & Anim Husb Res Mexico, Natl Inst Forestry, Natl Ctr Disciplinary Res Soil Water Plant Atmosp, Km 6-5 Canal Sacramento, Gomez Palacio, Durango, Mexico. EM sanchez.ignacio@inifap.gob.mx; diaz.gabriel@inifap.gob.mx; velasquez.agustin@inifap.gob.mx; slackd@email.arizona.edu; phil.heilman@ars.usda.gov; apedroza@chapingo.uruza.edu.mx FU Durango Foundation (Fundacion Produce Durango); National Institute for Forestry, Animal Husbandry and Agricultural Research of Mexico (INIFAP) FX The authors want to thank to the Durango Foundation (Fundacion Produce Durango) and the National Institute for Forestry, Animal Husbandry and Agricultural Research of Mexico (INIFAP) for their economic support to undergo this project. NR 27 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUN PY 2015 VL 114 BP 178 EP 188 DI 10.1016/j.compag.2015.03.009 PG 11 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA CL8RI UT WOS:000357241400019 ER PT J AU Mbonimpa, EG Gautam, S Lai, L Kumar, S Bonta, JV Wang, X Rafique, R AF Mbonimpa, E. G. Gautam, S. Lai, L. Kumar, S. Bonta, J. V. Wang, X. Rafique, R. TI Combined PEST and Trial-Error approach to improve APEX calibration SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE APEX; PEST; Trial and error; Calibration; Surface runoff ID HYDROLOGIC-MODELS; WATER-QUALITY; RUNOFF; CROPLAND; OPTIMIZATION; CONSERVATION; VALIDATION; LANDSCAPES; SIMULATION; EROSION AB The Agricultural Policy Environmental eXtender (APEX), a comprehensive hydrologic model well-suited for small watersheds, requires understanding of the input parameters for improved calibration. The "trial and error" method for calibrating the APEX model has been used very commonly in previous studies. In this study, the automatic calibration software Parameter Estimation (PEST) was combined with the conventional trial-and-error method to improve APEX calibration. The proposed Combined PEST and Trial Error (CPTE) approach can overcome: (i) weaknesses of "Trial Error' method in terms of tediousness and subjectivity involved in the decision to end a calibration, and (ii) drawback of PEST in that it may lead to biased simulation due to ignoring local specific condition. A case study was developed to verify the CPTE approach. The results based on APEX runoff simulation indicate that the CPTE approach greatly improved the calibration of APEX model with respect to model performance criteria. Coupling inverse modeling and trial error manual method can be an efficient and effective alternative in calibrating the APEX model. (C) 2015 Elsevier B.V. All rights reserved. C1 [Mbonimpa, E. G.; Gautam, S.; Lai, L.; Kumar, S.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Mbonimpa, E. G.] US Air Force, Dept Syst Engn & Management, Inst Technol, Wright Patterson AFB, OH 45433 USA. [Bonta, J. V.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Wang, X.] Texas AgriLife Res, Blackland Res & Extens Ctr, Temple, TX USA. [Rafique, R.] Pacific NW Natl Lab, Joint Global Change Res Inst, College Pk, MD 20740 USA. [Gautam, S.] Univ Missouri, Dept Bioengn, Columbia, MO 56211 USA. RP Kumar, S (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM Sandeep.Kumar@sdstate.edu FU USDA-NIFA [OHO01089-SS] FX Authors would like to acknowledge the US Department of Agriculture (USDA)-ARS personnel for providing hydrology data for this study. This research is part of a project supported by the USDA-NIFA, Project No. OHO01089-SS, "Quantifying the spatial location of small-scale land management changes in large watersheds using hydrological modeling". NR 50 TC 1 Z9 1 U1 5 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUN PY 2015 VL 114 BP 296 EP 303 DI 10.1016/j.compag.2015.04.014 PG 8 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA CL8RI UT WOS:000357241400031 ER PT J AU Duerr, AE Miller, TA Lanzone, M Brandes, D Cooper, J O'Malley, K Maisonneuve, C Tremblay, JA Katzner, T AF Duerr, Adam E. Miller, Tricia A. Lanzone, Michael Brandes, David Cooper, Jeff O'Malley, Kieran Maisonneuve, Charles Tremblay, Junior A. Katzner, Todd TI Flight response of slope-soaring birds to seasonal variation in thermal generation SO FUNCTIONAL ECOLOGY LA English DT Article DE Aquila chrysaetos; energy-limited; golden eagle; migration; orographic lift; soaring; thermal lift; time-limited; weather ID MIGRATING RAPTORS; WEATHER; SPEED; DEPARTURE; ALTITUDES; PATTERNS; ISRAEL AB 1. Animals respond to a variety of environmental cues, including weather conditions, when migrating. Understanding the relationship between weather and migration behaviour is vital to assessing time-and energy limitations of soaring birds. Different soaring modes have different efficiencies, are dependent upon different types of subsidized lift and are weather dependent. 2. We collected GPS locations from 47 known-age golden eagles that moved along 83 migration tracks. We paired each location with weather to determine meteorological correlates of migration during spring and fall as birds crossed three distinct ecoregions in north-east North America. 3. Golden eagle migration was associated with weather conditions that promoted thermal development, regardless of season, ecoregion or age. Eagle migration showed age-and season-specific responses to weather conditions that promoted orographic lift. 4. In spring, adult eagles migrated earlier, over fewer days, and under more variable weather conditions than did pre-adults, suggesting that adults were time limited and pre-adults made choices to conserve energy. In fall, we found no difference in the time span of migration or when each age class migrates; however, we saw evidence that pre-adults were less efficient migrants than adults. 5. The decision by soaring birds to migrate when thermals developed allowed individuals to manage trade-offs between migratory speed and migratory efficiency. When time was limited (i.e. spring movement of adults speeding towards nesting territories), use of whatever lift was available decreased the time span of migration. When migration was not time limited (e.g. spring movements by pre-adults, all movements in fall), eagles avoided suboptimal flight conditions by pausing migration, thus increasing the time span of migration while reducing energetic costs. C1 [Duerr, Adam E.; Miller, Tricia A.; Katzner, Todd] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Lanzone, Michael] Cellular Tracking Technol LLC, Somerset, PA 15501 USA. [Brandes, David] Lafayette Coll, Acopian Engn Ctr, Dept Civil & Environm Engn, Easton, PA 18042 USA. [Cooper, Jeff] Virginia Dept Game & Inland Fisheries, Fredericksburg, VA 22401 USA. [O'Malley, Kieran] West Virginia Div Nat Resources, Romney, WV 26575 USA. [Maisonneuve, Charles] Minist Dev Durable Environm Faune & Parcs, Rimouski, PQ G5L 8B3, Canada. [Tremblay, Junior A.] Minist Dev Durable Environm Faune & Parcs, Quebec City, PQ G1S 4X4, Canada. [Katzner, Todd] US Forest Serv, USDA, Timber & Watershed Lab, Parsons, WV 26287 USA. RP Duerr, AE (reprint author), W Virginia Univ, Div Forestry & Nat Resources, 322 Percival Hall, Morgantown, WV 26506 USA. EM adam.duerr@mail.wvu.edu OI Katzner, Todd/0000-0003-4503-8435 FU PA SWG [T-12, T47-R-1]; US DoE [DE-EE0003538]; NASA Climate and Biological Response Grant [NNX11AP61G]; Charles A. and Anne Morrow Lindbergh Foundation; Hydro-Quebec; Virginia Department of Game and Inland Fisheries through a Federal Aid in Wildlife Restoration grant from the US Fish and Wildlife Service FX Funding for this work was received from PA SWG grants T-12 and T47-R-1, US DoE grant DE-EE0003538, NASA Climate and Biological Response Grant #NNX11AP61G, Charles A. and Anne Morrow Lindbergh Foundation, Hydro-Quebec, and the authors' organizations. This publication was completed in part with funds provided by the Virginia Department of Game and Inland Fisheries through a Federal Aid in Wildlife Restoration grant from the US Fish and Wildlife Service. This work is Scientific Article No. 3222 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown. Use of golden eagles for this research was approved by the West Virginia University Institutional Animal Care and Use Committee (IACUC) protocol #11-0304 and Animal Care Committee of the Ministere du developpement durable, de l'Environnement, de la Faune et des Parcs du Quebec [MDDEFP] (permits CPA-FAUNE-07-00-02 and CPA-FAUNE-2009-06). TK and ML are both part owners of Cellular Tracking Technologies, LLC that manufacture GPS-GSM transmitters used in this project. We thank many people and organizations for their assistance with fieldwork and data collection. These include T. Anderson, the Bonta Family, B. Baillargeon, P. Beaupre, D. Brauning, J. Buchan, E. Estell, R. Faubert, R. Hartzel, B. Knight, D. Kramer, S. Jones, A. Mack, C. McCurdy, R. Mc Nicoll, B. D. Miller Family, P. Reum, B. Sargent, D. Smith, R. Tallman and M. Wheeler, the National Aviary, the Carnegie Museums of Natural History. We thank three anonymous reviewers for comments that greatly helped improve this manuscript. NR 43 TC 6 Z9 6 U1 2 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-8463 EI 1365-2435 J9 FUNCT ECOL JI Funct. Ecol. PD JUN PY 2015 VL 29 IS 6 BP 779 EP 790 DI 10.1111/1365-2435.12381 PG 12 WC Ecology SC Environmental Sciences & Ecology GA CM5OL UT WOS:000357737500006 ER PT J AU Mehring, AS Kuehn, KA Thompson, A Pringle, CM Rosemond, AD First, MR Lowrance, RR Vellidis, G AF Mehring, Andrew S. Kuehn, Kevin A. Thompson, Aaron Pringle, Catherine M. Rosemond, Amy D. First, Matthew R. Lowrance, R. Richard Vellidis, George TI Leaf litter nutrient uptake in an intermittent blackwater river: influence of tree species and associated biotic and abiotic drivers SO FUNCTIONAL ECOLOGY LA English DT Article DE aquatic hyphomycete; biofilm; chitin; coupled biogeochemical cycle; fungi; glucosamine; metal oxide; stoichiometry ID ORGANIC-MATTER; AQUATIC ENVIRONMENTS; MICROBIAL BIOFILMS; NITROGEN-FIXATION; BIOMASS; STREAM; SOIL; BIOAVAILABILITY; DECOMPOSITION; PHOSPHORUS AB 1. Organic matter may sequester nutrients as it decomposes, increasing in total N and P mass via multiple uptake pathways. During leaf litter decomposition, microbial biomass and accumulated inorganic materials immobilize and retain nutrients, and therefore, both biotic and abiotic drivers may influence detrital nutrient content. We examined the relative importance of these types of nutrient immobilization and compared patterns of nutrient retention in recalcitrant and labile leaf litter. 2. Leaf packs of water oak (Quercus nigra), red maple (Acer rubrum) and Ogeechee tupelo (Nyssa ogeche) were incubated for 431 days in an intermittent blackwater stream and periodically analysed for mass loss, nutrient and metal content, and microbial biomass. These data informed regression models explaining temporal changes in detrital nutrient content. Informal exploratory models compared estimated biologically associated nutrient stocks (fungal, bacterial, leaf tissue) to observed total detrital nutrient stocks. We predicted that (i) labile and recalcitrant leaf litter would act as sinks at different points in the breakdown process, (ii) plant and microbial biomass would not account for the entire mass of retained nutrients, and (iii) total N content would be more closely approximated than total P content solely from nutrients stored in leaf tissue and microbial biomass, due to stronger binding of P to inorganic matter. 3. Labile litter had higher nutrient concentrations throughout the study. However, lower mass loss of recalcitrant litter facilitated greater nutrient retention over longer incubations, suggesting that it may be an important long-term sink. N and P content were significantly related to both microbial biomass and metal content, with slightly stronger correlation with metal content over longer incubations. 4. Exploratory models demonstrated that a substantial portion of detrital nutrients was not accounted for by living or dead plant and microbial biomass, especially in the case of N. This suggests increased importance of both N and P sorption to inorganic matter over time, with possible additional storage of N complexed with lignin. A better understanding of the influence of these mechanisms may improve our understanding of detrital nutrient uptake, basal resource quality and retention and transport of nutrients in aquatic ecosystems. C1 [Mehring, Andrew S.; Pringle, Catherine M.; Rosemond, Amy D.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Kuehn, Kevin A.] Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. [Thompson, Aaron; Vellidis, George] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [First, Matthew R.] Woods Hole Oceanog Inst, Dept Geol & Geophys, Woods Hole, MA 02543 USA. [Lowrance, R. Richard] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. RP Mehring, AS (reprint author), Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92037 USA. EM amehring@ucsd.edu OI First, Matthew/0000-0003-1330-3353; First, Matt/0000-0003-3465-2376 FU USDA-CSREES Integrated Research, Education and Extension Competitive Grants Program's National Integrated Water Quality Program [2004-5113002224]; USDA-ARS CRIS; Odum School of Ecology, University of Georgia FX This work was funded by the USDA-CSREES Integrated Research, Education and Extension Competitive Grants Program's National Integrated Water Quality Program (Award No. 2004-5113002224), Hatch & State funds allocated to the Georgia Agricultural Experiment Stations, USDA-ARS CRIS project funds, and a Student Research Grant awarded to Andrew Mehring from the Odum School of Ecology, University of Georgia. Helpful comments on earlier versions of this manuscript were provided by John Davis and the Pringle and Rosemond laboratory groups at the University of Georgia. Seth Wenger provided valuable feedback on R coding and statistical analysis. We would like to thank Tom Maddox and Lisa Dean in the Analytical Chemistry Laboratory of the University of Georgia's Odum School of Ecology for analysis of plant litter carbon, nitrogen and phosphorus concentrations. We would also like to thank M. Jason Todd, Mamadou Coulibaly, Cynthia Tant, Katrina Morris, Jason Wisniewski and John Kominoski for providing assistance in the field. Chris Clegg, Debbie Coker and Leila Hargett assisted with water carbon and nutrient analyses, and Nehru Mantripragada and Gene Weeks assisted with metal analysis. We are grateful to Zachary Aultman and the Weyerhauser Company for granting access to their land. NR 63 TC 3 Z9 3 U1 13 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-8463 EI 1365-2435 J9 FUNCT ECOL JI Funct. Ecol. PD JUN PY 2015 VL 29 IS 6 BP 849 EP 860 DI 10.1111/1365-2435.12399 PG 12 WC Ecology SC Environmental Sciences & Ecology GA CM5OL UT WOS:000357737500014 ER PT J AU Kim, H Han, D Kang, J Choi, Y Levi, A Lee, GP Park, Y AF Kim, Hyeogjun Han, Dongyeop Kang, Jumsoon Choi, Youngwhan Levi, Amnon Lee, Gung Pyo Park, Younghoon TI Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.) SO HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY LA English DT Article DE cucurbits; DNA marker; fruit-related trait; marker-assisted breeding; maker-assisted selection ID INHERITANCE; RESISTANCE; REGIONS; KOREA AB The inheritance of foreground stripe pattern in rind of watermelon fruits [Citrullus lanatus (Thunb.) Matsum. & Nakai] was evaluated and molecular markers for selecting the Jubilee-type (JT) stripe pattern were developed based on bulked segregant analysis (BSA). Divergence in rind pattern among F-2 progeny derived from crossing Crimson-type (CT) 'Arka Manik' (AM) with JT 'TS34' (TS) indicated that stripe pattern is a quantitative trait controlled by more than one gene. The BSA of F-2 plants (derived from a cross between 'AM' and 'TS') using 60 random amplified polymorphic DNA (RAPD) primers revealed a distinct RAPD band (AT14-900) polymorphic between 'AM' (CT) and 'TS' (JT). The AT14-900 sequence (925 bp) was blasted to the reference watermelon (97103) genome and high sequence similarity (97.8%) was identified on physical location of 26246077 to 26246993 bp on chromosome 6. Two expressed sequence tags (ESTs) designated 'wsbin6-10' and 'wsbin6-11' that were closely linked to AT14-900 on a genetic linkage map (developed using the F-2 population derived from 'AM' x 'TS') were positioned 2,216 kb and 71 kb from AT14-900, respectively on the reference watermelon genome sequence. Marker genotyping of the F-2 population showed that wsbin6-11 was tightly linked to the JT stripe pattern of 'TS' and could be a useful codominant marker for selecting this trait. In a test using 100 breeding lines, 34 of the 36 lines carrying the JT stripe pattern were homozygous for the wsbin6-11 marker (450 bp) derived from 'TS', while other lines (e.g., with no stripe or CT stripe pattern) were homozygous for the wsbin6-11 marker (420 bp) derived 'AM'. These results indicated that wsbin6-11 would be a useful marker in watermelon breeding programs aiming to select for the JT stripe pattern from other various foreground and background rind patterns. C1 [Kim, Hyeogjun; Han, Dongyeop; Kang, Jumsoon; Choi, Youngwhan; Park, Younghoon] Pusan Natl Univ, Dept Hort Biosci, Miryang 627706, South Korea. [Levi, Amnon] ARS, US Vegetable Lab, USDA, Charleston, SC USA. [Lee, Gung Pyo] Chung Ang Univ, Dept Integrat Plant Sci, Coll Biotechnol & Nat Resources, Ansung 456756, South Korea. [Park, Younghoon] Pusan Natl Univ, Life & Ind Convergence Res Inst, Miryang 627706, South Korea. RP Park, Y (reprint author), Pusan Natl Univ, Dept Hort Biosci, Miryang 627706, South Korea. EM ypark@pusan.ac.kr FU Vegetable Breeding Research Center through Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs [710001-07-5]; Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA); Ministry of Oceans and Fisheries (MOF); Rural Development Administration (RDA); Korea Forest Service (KFS) FX This work was supported by a grant (710001-07-5) from the Vegetable Breeding Research Center through Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs. This research was also supported by Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS). NR 16 TC 1 Z9 1 U1 3 U2 15 PU KOREAN SOC HORTICULTURAL SCIENCE PI JEOLLABUK-DO PA C/O NATL INST HORTICULTURAL & HERBAL SCIENCE, 100, NONGSAENGMYEONG-RO, ISEO-MYEON, WANJU-GUN, JEOLLABUK-DO, 565-852, SOUTH KOREA SN 2211-3452 EI 2211-3460 J9 HORTIC ENVIRON BIOTE JI Hortic. Environ. Biotechnol. PD JUN PY 2015 VL 56 IS 3 BP 341 EP 349 DI 10.1007/s13580-015-0017-1 PG 9 WC Horticulture SC Agriculture GA CM5ST UT WOS:000357749600010 ER PT J AU Hummer, KE Hancock, JF AF Hummer, Kim E. Hancock, Jim F. TI Vavilovian Centers of Plant Diversity: Implications and Impacts SO HORTSCIENCE LA English DT Article DE center of diversity; center of origin; plant evolution; germplasm; plant breeding; plant exploration AB N.I. Vavilov, Academician of the V.I. Lenin All-Union Academy of Agricultural Sciences, takes his place as a founding pillar of knowledge in the cathedral of the origin and development of cultivated plants. Relying on the theories of his revered predecessors, Charles Darwin and Alphonse de Candolle, Vavilov developed the concept of centers of origin for agronomic crops. Vavilov's astute hypotheses set the stage for the modern use of exotic germplasm in plant breeding. Vavilov, a devoted scientist, continued to revise and refine his theory of the centers of origin throughout his lifetime based on additional plant collections and data evaluation. Though he initially proposed three in 1924, and eight in 1935, his final papers of 1940, discussed seven major centers with some minor additions. His concept of specific centers of origin for crop plants was not an isolated aphorism but has directed breeders, on their study and reflection, to the continued improvement and economic development of plants for humanity. Inherent genetic plant variability is the basis of domestication and breeding into crops of economic importance with food, fuel, fiber, and industrial uses. The objective of this article is to present a summary of Vavilov's plant explorations. His collection trips led to the development of his theory of the centers of origin of cultivated crops, the law of homologous variation, as well as his concept of genetic erosion. Further modification of his theories by other scientists and the impact of his ideas are mentioned. His influence on present day conservation of genetic resources are presented. C1 [Hummer, Kim E.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Hancock, Jim F.] Michigan State Univ, E Lansing, MI 48824 USA. RP Hummer, KE (reprint author), USDA ARS Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM Kim.Hummer@ars.usda.gov NR 17 TC 2 Z9 2 U1 2 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2015 VL 50 IS 6 BP 780 EP 783 PG 4 WC Horticulture SC Agriculture GA CM5YY UT WOS:000357766100003 ER PT J AU Hummer, KE AF Hummer, Kim E. TI In the Footsteps of Vavilov: Plant Diversity Then and Now SO HORTSCIENCE LA English DT Article DE center of diversity; center of origin; germplasm; plant breeding; plant evolution; plant exploration AB N.I. Vavilov's theories direct present-day global activities in plant science, breeding, and conservation. His expeditions around the world located centers of diversity of crop evolution. Vavilov was one of the earliest scientists to realize that wild genetic diversity could be lost, through genetic erosion, reducing the possibilities for future crop improvement. To measure genetic erosion, Gary Nabhan and colleagues traveled in 11 countries following routes that Vavilov had taken more than half a century before. The detailed notes concerning the vegetation and flora that Vavilov observed could be used as a baseline in contrast with Nabhan's plant and cultivar inventories to observe changes in plant diversity at specific sites. The objective of this manuscript is to summarize potential genetic erosion at three case study locations, the Pamiri Highlands of Tajikistan, the Ethiopian Highlands, and the Colorado Plateau of Southwestern North America. At these localities Vavilov's notes can be compared with the agricultural activities of the modern day. In each case, significant climatic, environmental, and human-caused changes have affected the local agriculture during the intervening years. Localities that have retained diversity have Suffered the least. Reduction of diversity is associated with decreased agricultural stability and productivity. Programs encouraging farmers to manage diversity and promote involvement of local youth in agriculture may reduce or moderate the effect of genetic erosion. C1 USDA ARS NCGR, Corvallis, OR 97333 USA. RP Hummer, KE (reprint author), USDA ARS NCGR, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM Kim.Hummer@ars.usda.gov NR 9 TC 1 Z9 1 U1 3 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2015 VL 50 IS 6 BP 784 EP 788 PG 5 WC Horticulture SC Agriculture GA CM5YY UT WOS:000357766100004 ER PT J AU Vargas, OL Bryla, DR Weiland, JE Strik, BC Sun, L AF Vargas, Oscar L. Bryla, David R. Weiland, Jerry E. Strik, Bernadine C. Sun, Luna TI Irrigation and Fertigation with Drip and Alternative Micro Irrigation Systems in Northern Highbush Blueberry SO HORTSCIENCE LA English DT Article DE Vaccinium corymbosum; Phytophthora cinnamomi; geotextile irrigation system; microsprinklers; nutrient management; root rot ID FERTILIZER APPLICATION; GRANULAR FERTILIZER; WATER REQUIREMENTS; PYTHIUM INFECTION; FRUIT PRODUCTION; GROWTH; NITROGEN; ESTABLISHMENT; AVAILABILITY; PHYTOPHTHORA AB The use of conventional drip and alternative micro irrigation systems were evaluated for 3 years in six newly planted cultivars (Earliblue, Duke, Draper, Bluecrop, Elliott, and Aurora) of northern highbush blueberry (Vaccinium corymbosum L.). The drip system included two lines of tubing on each side of the row with in-line drip emitters at every 0.45 m. The alternative systems included geotextile tape and microsprinklers. The geotextile tape was placed alongside the plants and dispersed water and nutrients over the entire length. Microsprinklers were installed between every other plant at a height of 1.2 m. Nitrogen was applied by fertigation at annual rates of 100 and 200 kg.ha(-1) N by drip, 200 kg.ha(-1) N by geotextile tape, and 280 kg.ha(-1) N by micro-sprinklers. By the end of the first season, plant size, in terms of canopy cover, was greatest with geotextile tape, on average, and lowest with microsprinklers or drip at the lower N rate. The following year, canopy cover was similar with geotextile tape and drip at the higher N rate in each cultivar, and was lowest with microsprinklers in all but 'Draper'. In most of the cultivars, geotextile tape and drip at the higher N rate resulted in greater leaf N concentrations than microsprinklers or drip at the lower N rate, particularly during the first year after planting. By the third year, yield averaged 3.1-9.1 t.ha(-1) among the cultivars, but was similar with geotextile tape and drip at either N rate, and was only lower with microsprinklers. Overall, drip was more cost effective than geotextile tape, and fertigation with 100 kg.ha(-1) N by drip was sufficient to maximize early fruit production in each cultivar. Microsprinklers were less effective by comparison and resulted in white salt deposits on the fruit. C1 [Vargas, Oscar L.; Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Bryla, David R.; Weiland, Jerry E.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Sun, Luna] Oregon State Univ, Dept Stat, Corvallis, OR 97331 USA. RP Bryla, DR (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM david.bryla@ars.usda.gov NR 33 TC 2 Z9 2 U1 3 U2 16 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2015 VL 50 IS 6 BP 897 EP 903 PG 7 WC Horticulture SC Agriculture GA CM5YY UT WOS:000357766100022 ER PT J AU Stringer, SJ Draper, AD Marshall-Shaw, D Sampson, BJ Adamczyk, JJ AF Stringer, Stephen J. Draper, Arlen D. Marshall-Shaw, Donna Sampson, Blair J. Adamczyk, John J., Jr. TI 'Bluesfest' Rabbiteye Blueberry SO HORTSCIENCE LA English DT Editorial Material DE Vaccinium ashei; V. virgatum; fruit breeding C1 [Stringer, Stephen J.; Draper, Arlen D.; Marshall-Shaw, Donna; Sampson, Blair J.; Adamczyk, John J., Jr.] ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. RP Stringer, SJ (reprint author), ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. EM Stephen.Stringer@ars.usda.gov NR 4 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2015 VL 50 IS 6 BP 934 EP 935 PG 2 WC Horticulture SC Agriculture GA CM5YY UT WOS:000357766100030 ER PT J AU Fernandez-Salvador, J Strik, BC Bryla, DR AF Fernandez-Salvador, Javier Strik, Bernadine C. Bryla, David R. TI Response of Blackberry Cultivars to Fertilizer Source during Establishment in an Organic Fresh Market Production System SO HORTTECHNOLOGY LA English DT Article DE Rubus; pelletized soy meal; processed poultry litter; fish emulsion hydrolysate; molasses; fruit quality; Brix; percent soluble solids ID ARAPAHO THORNLESS BLACKBERRY; MARION TRAILING BLACKBERRY; WEED MANAGEMENT-PRACTICES; RED RASPBERRY; BORON FERTILIZATION; CHEMICAL-PROPERTIES; NITROGEN UPTAKE; SOIL FERTILITY; YIELD; QUALITY AB Blackberry (Rubus ssp. Rubus) cultivars, three trailing types (Marion, Black Diamond, and Obsidian) and one semierect type (Triple Crown), were studied for their response to different types of fertilizer from 2011-12, at a certified organic, grower collaborator site located in Jefferson, OR. Plants were fertilized at a target rate of 50 1b/acre nitrogen (N) each spring using three different sources: 1) a liquid fish and molasses blend (4N 0P-1.7K); 2) pelletized soy (Glycine max) meal (8N-0.4P-1.7K); and 3) pelletized, processed poultry litter (4N-1.3P-2.5K). Plants were drip irrigated, and weeds were managed using a polypropylene, permeable landscape fabric (weed mat). Plant responses were greatly affected by cultivar, whereas the effects of fertilizer type were relatively minor. 'Triple Crown' produced the greatest yield in both years, whereas 'Black Diamond' and 'Marion' had the lowest yield in 2011 and 2012, respectively. 'Triple Crown' fruit had the highest percent soluble solids and were the least firm in 2011, whereas 'Marion' fruit were the least firm in 2012. Harvest date, within year, affected the fruit quality variables measured in all cultivars. Most soil nutrient levels were within the recommended range for all fertilizer treatments, except for boron (B), which declined to deficient levels in the second year. Fertilizer type had no effect on soil nutrient levels other than fertilization with the fish and molasses blend product increased soil potassium and sodium. Soil nutrient levels were affected by cultivar but varied by year for many nutrients. Primocane leaf tissue nutrient concentrations were above or within recommended standards for most nutrients, except for magnesium (Mg), calcium (Ca), and B, which, depending on the cultivar, were below standards. Over the 2-year study, the blackberry cultivars responded similarly to the three types of organic fertilizer. However, the cost of N varied from $8.16/1b for the liquid fish and molasses blend, $5.35/1b for the pelletized soy meal, and $2.54/1b for the pelletized, processed poultry litter. Supplemental fertilization with B, Mg, and Ca would be required with each fertilizer studied to maintain recommended soil fertility levels. C1 [Fernandez-Salvador, Javier; Strik, Bernadine C.] Oregon State Univ, Coll Agr Sci, Dept Hort, Corvallis, OR 97331 USA. [Bryla, David R.] USDA ARS, Corvallis, OR 97330 USA. RP Strik, BC (reprint author), Oregon State Univ, Coll Agr Sci, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. EM Bernadine.strik@oregonstate.edu NR 58 TC 6 Z9 6 U1 4 U2 14 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2015 VL 25 IS 3 BP 277 EP 292 PG 16 WC Horticulture SC Agriculture GA CM6AR UT WOS:000357770600002 ER PT J AU Dami, IE Li, SX Bowen, PA Bogdanoff, CP Shellie, KC Willwerth, J AF Dami, Imed E. Li, Shouxin Bowen, Patricia A. Bogdanoff, Carl P. Shellie, Krista C. Willwerth, Jim TI Foliar Applied Abscisic Acid Increases 'Chardonnay' Grapevine Bud Freezing Tolerance during Autumn Cold Acclimation SO HORTTECHNOLOGY LA English DT Article DE Vitis vinifera; hardiness; differential thermal analysis; cold injury ID ABA; SENESCENCE; STRATEGIES; RESPONSES; DORMANCY; INJURY; L. AB Economic loss due to cold weather events is a major constraint to winegrape (Vitis yinifera) production and wine-related industries where extreme and/or fluctuating winter temperatures induce injury and require remedial retraining and replanting increases production costs and lowers yield and fruit quality. The purpose of this study was to determine whether a foliar application of abscisic acid (ABA) could increase the freezing tolerance (FT) of field-grown, 'Chardonnay' winegrape and whether its effectiveness can be influenced by the phenological timing of the application. Mature 'Chardonnay' grapevines were treated with a foliar application of ABA at a concentration of 500 at vine phenological stages corresponding to 50% veraison, postveraison, and postharvest. Results from field trial sites located in four distinct winegrape production regions in the United States (Idaho and Ohio) and Canada (British Columbia and Ontario) showed that foliar application of ABA increased bud FT, primarily during autumn cold acclimation. Foliar ABA application had no consistent influence on bud FT in midwinter or during spring deacdimation, or on percent budburst in spring. Vine phenological stage at the time of ABA foliar application influenced ABA effectiveness, although results were inconsistent among locations. At most locations, applications made at veraison or postveraison were more effective than applications made postharvest. No phytotoxic response or adverse changes in yield or berry composition were detected in response to ABA application. The consistent increase in bud FT during autumn cold acclimation observed at all trial locations in this study indicates that foliar ABA, applied at veraison or postveraison, can reduce the risk of economic loss due to cold injury in production regions with frequent early autumn cold weather events. C1 [Dami, Imed E.; Li, Shouxin] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Bowen, Patricia A.; Bogdanoff, Carl P.] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC V0H 1Z0, Canada. [Shellie, Krista C.] USDA ARS, Hort Crops Res Unit, Parma, ID 83660 USA. [Willwerth, Jim] Brock Univ, Cool Climate Oenol & Viticulture Inst, St Catharines, ON L2S 3A1, Canada. RP Dami, IE (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. EM dami.1@osu.edu FU Agricultural Research Service Project [5358-21000-034-00D]; British Columbia Wine Grape Council; Agriculture and Agri-Food Canada; Ohio Grape Industries Program; Ohio State University Department of Horticulture and Crop Science FX This research was supported by funding from Agricultural Research Service Project 5358-21000-034-00D entitled "Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest"; the British Columbia Wine Grape Council and Agriculture and Agri-Food Canada; the Ohio Grape Industries Program, and the Ohio State University Department of Horticulture and Crop Science. We thank Alan Muir, Monte Shields, and Cheryl Franklin-Miller for their technical expertise and Winemakers LLC and Meineke Vineyards for the use of their field resources and materials. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 30 TC 1 Z9 1 U1 0 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2015 VL 25 IS 3 BP 293 EP 305 PG 13 WC Horticulture SC Agriculture GA CM6AR UT WOS:000357770600003 ER PT J AU Ward, B Smith, P James, S Stansell, Z Farnham, M AF Ward, Brian Smith, Powell James, Susan Stansell, Zachary Farnham, Mark TI Increasing Plant Density in Eastern United States Broccoli Production Systems to Maximize Marketable Head Yields SO HORTTECHNOLOGY LA English DT Article DE Brassica oleracea; plant spacing; plant population AB Increased demand for fresh market crown-cut broccoli (Brassica oleracea var. italica) has led to increased production along the eastern seaboard of the United States. Maximizing broccoli yields is a primary concern for quickly expanding eastern markets. Thus, a plant density study was carried out in Fall 2012 and 2013 using the hybrid cultivar Emerald Crown on a commercial farm in Summerton, SC, and in Fall 2013 using 'Emerald Crown' and another hybrid, 'Durapak 19', on a research farm in Charleston, SC. The objective was to determine the effect of variable within-row spacings of 4, 6, and 8 inches (using a system with double rows spaced 12-inches apart) on marketable yields and quality in the three environments. Our results indicated that increasing plant density by reducing within-row spacing to 4 inches significantly increased overall yield per hectare over the 6- and 8-inch spacing treatments at two of three environments. Stem diameter and average head weight were unaffected by plant density; however, heads harvested from plots with the highest plant densities had significantly (P < 0.01) lower bead uniformity in the Summerton 2012 trial, and significantly (P < 0.05) larger bead size in the two trials conducted in 2013. In general, the highest total marketable yields were from the 4-inch within-row spacing, but increased competition at the highest density may increase the risk of plants producing heads with lower quality characteristics. With the increased risk of producing lower quality marketable heads along with the increased production costs associated with the 4-inch spacing, yield and head quality attributes may be optimized at the 6-inch within-row spacing. C1 [Ward, Brian] Clemson Univ, Coastal Res & Educ Ctr, Dept Hort, Charleston, SC 29414 USA. [Smith, Powell; James, Susan] Clemson Univ Cooperat Extens, Lexington Cty Extens Ctr, Lexington, SC 29072 USA. [Stansell, Zachary; Farnham, Mark] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Ward, B (reprint author), Clemson Univ, Coastal Res & Educ Ctr, Dept Hort, 2700 Savannah Highway, Charleston, SC 29414 USA. EM bw@clemson.edu FU U.S. Department of Agriculture-National Institute of Food and Agriculture-Specialty Crops Research Initiative grant FX This research was partially funded by a U.S. Department of Agriculture-National Institute of Food and Agriculture-Specialty Crops Research Initiative grant entitled "Developing an Eastern Broccoli Industry." NR 14 TC 2 Z9 2 U1 1 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2015 VL 25 IS 3 BP 330 EP 334 PG 5 WC Horticulture SC Agriculture GA CM6AR UT WOS:000357770600007 ER PT J AU Panagopoulos, Y Gassman, PW Arritt, RW Herzmann, DE Campbell, TD Valcu, A Jha, MK Kling, CL Srinivasan, R White, M Arnold, JG AF Panagopoulos, Yiannis Gassman, Philip W. Arritt, Raymond W. Herzmann, Daryl E. Campbell, Todd D. Valcu, Adriana Jha, Manoj K. Kling, Catherine L. Srinivasan, Raghavan White, Michael Arnold, Jeffrey G. TI Impacts of climate change on hydrology, water quality and crop productivity in the Ohio-Tennessee River Basin SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE agricultural management scenarios; corn-based systems; global circulation models; hydrology; water quality; crop yields; SWAT; Ohio-Tennessee River Basin ID AGRICULTURAL CONSERVATION PRACTICES; GULF-OF-MEXICO; RUNOFF CURVE NUMBERS; MISSISSIPPI RIVER; ASSESSMENT-TOOL; MODEL DEVELOPMENT; SWAT; SOIL; YIELD; CALIBRATION AB Nonpoint source pollution from agriculture is the main source of nitrogen and phosphorus in the stream systems of the Corn Belt region in the Midwestern US. The eastern part of this region is comprised of the Ohio-Tennessee River Basin (OTRB), which is considered a key contributing area for water pollution and the Northern Gulf of Mexico hypoxic zone. A point of crucial importance in this basin is therefore how intensive corn-based cropping systems for food and fuel production can be sustainable and coexist with a healthy water environment, not only under existing climate but also under climate change conditions in the future. To address this issue, a OTRB integrated modeling system has been built with a greatly refined 12-digit subbasin structure based on the Soil and Water Assessment Tool (SWAT) water quality model, which is capable of estimating landscape and in-stream water and pollutant yields in response to a wide array of alternative cropping and/or management strategies and climatic conditions. The effects of three agricultural management scenarios on crop production and pollutant loads exported from the crop land of the OTRB to streams and rivers were evaluated: (1) expansion of continuous corn across the entire basin, (2) adoption of no-till on all corn and soybean fields in the region, (3) implementation of a winter cover crop within the baseline rotations. The effects of each management scenario were evaluated both for current climate and projected mid-century (2046-2065) climates from seven global circulation models (GCMs). In both present and future climates each management scenario resulted in reduced erosion and nutrient loadings to surface water bodies compared to the baseline agricultural management, with cover crops causing the highest water pollution reduction. Corn and soybean yields in the region were negligibly influenced from the agricultural management scenarios. On the other hand, both water quality and crop yield numbers under climate change deviated considerably for all seven GCMs compared to the baseline climate. Future climates from all GCMs led to decreased corn and soybean yields by up to 20% on a mean annual basis, while water quality alterations were either positive or negative depending on the GCM. The study highlights the loss of productivity in the eastern Corn Belt under climate change, the need to consider a range of GCMs when assessing impacts of climate change, and the value of SWAT as a tool to analyze the effects of climate change on parameters of interest at the basin scale. C1 [Panagopoulos, Yiannis; Gassman, Philip W.; Campbell, Todd D.; Valcu, Adriana; Kling, Catherine L.] Iowa State Univ, CARD, Ames, IA 50011 USA. [Arritt, Raymond W.; Herzmann, Daryl E.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Jha, Manoj K.] North Carolina A&T State Univ, Dept Civil Architectural & Environm Engn, Greensboro, NC 27411 USA. [Srinivasan, Raghavan] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [Srinivasan, Raghavan] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [White, Michael; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Panagopoulos, Y (reprint author), Iowa State Univ, CARD, Ames, IA 50011 USA. EM ypanag@iastate.edu; pwgassma@iastate.edu; rwarritt@bruce.agron.iastate.edu; akrherz@iastate.edu; tdc@iastate.edu; amvalcu@iastate.edu; mkjha@ncat.edu; ckling@iastate.edu; r-srinivasan@tamu.edu; mike.white@ars.usda.gov; jeff.arnold@ars.usda.gov RI Srinivasan, R/D-3937-2009 FU National Science Foundation [DEB1010259]; U.S. Department of Agriculture, National Institute of Food and Agriculture [20116800230190] FX This research was partially funded by the National Science Foundation, Award No. DEB1010259, Understanding Land Use Decisions & Watershed Scale Interactions: Water Quality in the Mississippi River Basin & Hypoxic Conditions in the Gulf of Mexico, and by the U.S. Department of Agriculture, National Institute of Food and Agriculture, Award No. 20116800230190, Climate Change, Mitigation, and Adaptation In Corn-Based Cropping Systems. NR 61 TC 5 Z9 5 U1 18 U2 89 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD JUN PY 2015 VL 8 IS 3 SI SI BP 36 EP 53 DI 10.3965/j.ijabe.20150803.1497 PG 18 WC Agricultural Engineering SC Agriculture GA CM2UD UT WOS:000357536900004 ER PT J AU Yen, H White, MJ Jeong, J Arabi, M Arnold, JG AF Yen, Haw White, Michael J. Jeong, Jaehak Arabi, Mazdak Arnold, Jeffrey G. TI Evaluation of alternative surface runoff accounting procedures using SWAT model SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE Soil and Water Assessment Tool (SWAT); curve number method; Bayesian model averaging; uncertainty analysis; hydrology; water quality ID WATER ASSESSMENT-TOOL; CURVE NUMBER; INPUT DATA; SOIL; UNCERTAINTY; SIMULATIONS; ENSEMBLES; BEHAVIOR; BASIN AB For surface runoff estimation in the Soil and Water Assessment Tool (SWAT) model, the curve number (CN) procedure is commonly adopted to calculate surface runoff by dynamically updating CN values based on antecedent soil moisture condition (SCSI) in field. From SWAT2005 and onward, an alternative approach has become available to apply the CN method by relating the runoff potential to daily evapotranspiration (SCSII). While improved runoff prediction with SCSII has been reported in several case studies, few investigations have been made on its influence to water quality output or on the model uncertainty associated with the SCSII method. The objectives of the research were: (1) to quantify the improvements in hydrologic and water quality predictions obtained through different surface runoff estimation techniques; and (2) to examine how model uncertainty is affected by combining different surface runoff estimation techniques within SWAT using Bayesian model averaging (BMA). Applications of BMA provide an alternative approach to investigate the nature of structural uncertainty associated with both CN methods. Results showed that SCSII and BMA associated approaches exhibit improved performance in both discharge and total NO3 predictions compared to SCSI. In addition, the application of BMA has a positive effect on finding well performed solutions in the multi-dimensional parameter space, but the predictive uncertainty is not evidently reduced or enhanced. Therefore, we recommend additional future SWAT calibration/validation research with an emphasis on the impact of SCSII on the prediction of other pollutants. C1 [Yen, Haw; Arabi, Mazdak] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Yen, Haw; Jeong, Jaehak] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Yen, Haw; White, Michael J.; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Yen, H (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. EM hyen@brc.tamus.edu; mike.white@ars.usda.gov; jjeong@brc.tamus.edu; mazdak.arabi@colostate.edu; jeff.arnold@ars.usda.gov FU USDA - National Institute of Food and Agriculture [2007-51130-03876, 2009-51130-06038]; Research Program for Agricultural Science & Technology Development [PJ008566]; National Academy of Agricultural Science, Rural Development Administration, Republic of Korea; USDA-NRCS Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components FX This study was supported in part by the USDA - National Institute of Food and Agriculture grants 2007-51130-03876, 2009-51130-06038, the Research Program for Agricultural Science & Technology Development (Project No. PJ008566), National Academy of Agricultural Science, Rural Development Administration, Republic of Korea, and the USDA-NRCS Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components. Awesome comments by the associate editor and reviewers greatly improved the quality of the manuscript. Thanks a lot! In addition, please remember that USDA is an equal opportunity employer and provider. NR 53 TC 9 Z9 9 U1 3 U2 13 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD JUN PY 2015 VL 8 IS 3 SI SI BP 54 EP 68 DI 10.3965/j.ijabe.20150803.833 PG 15 WC Agricultural Engineering SC Agriculture GA CM2UD UT WOS:000357536900005 ER PT J AU Taylor, RAJ Jeong, J White, M Arnold, JG AF Taylor, Robin A. J. Jeong, Jaehak White, Michael Arnold, Jeffrey G. TI Code modernization and modularization of APEX and SWAT watershed simulation models SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE code modernization; modularization; object-oriented programming; Fortran 2008; landscape-scale models; APEX; EPIC; SWAT ID EROSION; SOIL; PRODUCTIVITY; CALIBRATION; VALIDATION; HISTORY; SYSTEM; TOOL AB SWAT (Soil and Water Assessment Tool) and APEX (Agricultural Policy/Environmental eXtender) are respectively large and small watershed simulation models derived from EPIC (Environmental Policy Integrated Climate), a field-scale agroecology simulation model. All three models are coded in Fortran and have evolved over several decades. They are widely used to analyze anthropogenic influences on soil and water quality and quantity. Much of the original Fortran code has been retained even though Fortran has been through several cycles of development. Fortran now provides functionality originally restricted to languages like C, designed to communicate directly with the operating system and hardware. One can now use an object-oriented style of programming in Fortran, including inheritance, run-time polymorphism and overloading. In order to enhance their utility in research and policy-making, the models are undergoing a major revision to use some of the new Fortran features. With these new programming paradigms the developers of SWAT, APEX, and EPIC are working to make communication between the two models seamless. This paper describes the ongoing revision of these models that will make them easier to use, maintain, modify and document. It is intended that they will converge as they continue to evolution, while maintaining their distinctive features, capabilities and identities. C1 [Taylor, Robin A. J.; Jeong, Jaehak] Texas A&M AgriLife Res, Blackand Res & Extens Ctr, Temple, TX 76502 USA. [White, Michael; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Taylor, RAJ (reprint author), Texas A&M AgriLife Res, Blackand Res & Extens Ctr, Temple, TX 76502 USA. EM rtaylor@brc.tamus.edu; jjeong@brc.tamus.edu; mike.white@ars.usda.gov; jeff.arnold@ars.usda.gov NR 31 TC 1 Z9 1 U1 4 U2 15 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD JUN PY 2015 VL 8 IS 3 SI SI BP 81 EP 94 DI 10.3965/j.ijabe.20150803.1081 PG 14 WC Agricultural Engineering SC Agriculture GA CM2UD UT WOS:000357536900007 ER PT J AU Wang, DG Liu, YH AF Wang, Deguo Liu, Yanhong TI Development of Primer Sets for Loop-Mediated Isothermal Amplification that Enables Rapid and Specific Detection of Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article DE loop-mediated isothermal amplification (LAMP); 16S rRNA gene; 16S-23S rRNA intergenic spacer; Streptococcus dysgalactiae; Streptococcus uberis; Streptococcus agalactiae ID POLYMERASE-CHAIN-REACTION; BOVINE MASTITIS; STAPHYLOCOCCUS-AUREUS; SEQUENCE-ANALYSIS; ACID BACTERIA; IDENTIFICATION; GENE; PCR; SAMPLES; PATHOGENS AB Streptococcus dysgalactiae, Streptococcus uberis and Streptococcus agalactiae are the three main pathogens causing bovine mastitis, with great losses to the dairy industry. Rapid and specific loop-mediated isothermal amplification methods (LAMP) for identification and differentiation of these three pathogens are not available. With the 16S rRNA gene and 16S-23S rRNA intergenic spacers as targets, four sets of LAMP primers were designed for identification and differentiation of S. dysgalactiae, S. uberis and S. agalactiae. The detection limit of all four LAMP primer sets were 0.1 pg DNA template per reaction, the LAMP method with 16S rRNA gene and 16S-23S rRNA intergenic spacers as the targets can differentiate the three pathogens, which is potentially useful in epidemiological studies. C1 [Wang, Deguo] Xuchang Univ, Food & Bioengn Coll, Henan Postdoctoral Res Base, Xuchang 461000, Peoples R China. [Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Liu, YH (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM wangdg666@aliyun.com; yanhong.liu@ars.usda.gov FU Natural Science Foundation of China [31172331]; NSFC-Henan Talent Training Joint Foundation [U1204330]; Project for Scientific Research & Innovation Team of Henan Universities [15IRTSTHN016]; Foundation of Henan Province for Excellent Young Teachers of Colleges and Universities [2012GGJS-172]; Project of Xuchang University for Outstanding Young Backbone Teachers; Training Project for Outstanding Youth Backbone Personnel of Xuchang University FX The authors would like to acknowledge the China Scholarship Council. This work was supported by Natural Science Foundation of China (31172331), NSFC-Henan Talent Training Joint Foundation (U1204330), Project for Scientific Research & Innovation Team of Henan Universities (15IRTSTHN016), Foundation of Henan Province for Excellent Young Teachers of Colleges and Universities (2012GGJS-172), Project of Xuchang University for Outstanding Young Backbone Teachers, and Training Project for Outstanding Youth Backbone Personnel of Xuchang University. NR 28 TC 0 Z9 2 U1 2 U2 10 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD JUN PY 2015 VL 12 IS 6 BP 5735 EP 5742 DI 10.3390/ijerph120605735 PG 8 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA CL9BI UT WOS:000357268500003 PM 26016433 ER PT J AU Zayed, AB Britch, SC Soliman, MI Linthicum, KJ AF Zayed, Abdelbaset B. Britch, Seth C. Soliman, Mohamed I. Linthicum, Kenneth J. TI MOSQUITOES AND THE ENVIRONMENT IN NILE DELTA VILLAGES WITH PREVIOUS RIFT VALLEY FEVER ACTIVITY SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Larval habitat; arbovirus model; population dynamics; risk estimation; mosquito ecology ID VIRUS; EGYPT; OUTBREAK; KENYA; ISOLATIONS; LOGARITHMS AB Egypt is affected by serious human and animal mosquito-borne diseases such as Rift Valley fever (RVF). We investigated how potential RVF virus mosquito vector populations are affected by environmental conditions in the Nile Delta region of Egypt by collecting mosquitoes and environmental data from 3 key governorates before and after 2012 seasonal flooding. We found that environmental effects varied among species, life stages, pre- and postflood groupings, and geographic populations of the same species, and that mosquito community composition could change after flooding. Our study provides preliminary data for modeling mosquitoes and mosquito-borne diseases in the Nile Delta region. C1 [Zayed, Abdelbaset B.] Al Azhar Univ, Fac Sci, Girls Branch, Cairo, Egypt. [Britch, Seth C.; Linthicum, Kenneth J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Soliman, Mohamed I.] Gen Org Inst & Teaching Hosp, Minist Hlth, Res Inst Med Entomol, Giza, Egypt. RP Britch, SC (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. FU US Department of Agriculture-Agricultural Research Service Biosecurity Engagement Program [31060] FX This research was supported by US Department of Agriculture-Agricultural Research Service Biosecurity Engagement Program Project 31060, Environmental factors determining Rift Valley fever virus vectors in Egypt. The US Department of Agriculture is an equal opportunity provider and employer. NR 25 TC 0 Z9 0 U1 0 U2 5 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD JUN PY 2015 VL 31 IS 2 BP 139 EP 148 PG 10 WC Entomology SC Entomology GA CM5DM UT WOS:000357707000003 PM 26181689 ER PT J AU Winkel, LHE Vriens, B Jones, GD Schneider, LS Pilon-Smits, E Banuelos, GS AF Winkel, Lenny H. E. Vriens, Bas Jones, Gerrad D. Schneider, Leila S. Pilon-Smits, Elizabeth Banuelos, Gary S. TI Selenium Cycling Across Soil-Plant-Atmosphere Interfaces: A Critical Review SO NUTRIENTS LA English DT Review DE selenium; environment; plants; soil; atmosphere; speciation; biomethylation; biofortification; hyperaccumulation ID HYPERACCUMULATOR STANLEYA-PINNATA; AGRICULTURAL DRAINAGE SEDIMENT; EVAPORATION POND WATER; ORGANIC-MATTER CONTENT; PRAIRIE DOG HERBIVORY; TRITICUM-AESTIVUM L.; VOLATILE SELENIUM; HUMAN HEALTH; BRASSICA-JUNCEA; ISOTOPE ANALYSIS AB Selenium (Se) is an essential element for humans and animals, which occurs ubiquitously in the environment. It is present in trace amounts in both organic and inorganic forms in marine and freshwater systems, soils, biomass and in the atmosphere. Low Se levels in certain terrestrial environments have resulted in Se deficiency in humans, while elevated Se levels in waters and soils can be toxic and result in the death of aquatic wildlife and other animals. Human dietary Se intake is largely governed by Se concentrations in plants, which are controlled by root uptake of Se as a function of soil Se concentrations, speciation and bioavailability. In addition, plants and microorganisms can biomethylate Se, which can result in a loss of Se to the atmosphere. The mobilization of Se across soil-plant-atmosphere interfaces is thus of crucial importance for human Se status. This review gives an overview of current knowledge on Se cycling with a specific focus on soil-plant-atmosphere interfaces. Sources, speciation and mobility of Se in soils and plants will be discussed as well as Se hyperaccumulation by plants, biofortification and biomethylation. Future research on Se cycling in the environment is essential to minimize the adverse health effects associated with unsafe environmental Se levels. C1 [Winkel, Lenny H. E.; Vriens, Bas; Schneider, Leila S.] ETH, Swiss Fed Inst Technol ETH, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland. [Winkel, Lenny H. E.; Vriens, Bas; Jones, Gerrad D.] Eawag Swiss Fed Inst Aquat Sci & Technol, CH-8600 Dubendorf, Switzerland. [Pilon-Smits, Elizabeth] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Banuelos, Gary S.] ARS, USDA, San Joaquin Valley Agr Ctr, Parlier, CA 93648 USA. RP Winkel, LHE (reprint author), ETH, Swiss Fed Inst Technol ETH, Inst Biogeochem & Pollutant Dynam, CH-8092 Zurich, Switzerland. EM lenny.winkel@eawag.ch; bas.vriens@eawag.ch; gerrad.jones@eawag.ch; leilasc@student.ethz.ch; epsmits@lamar.colostate.edu; gary.banuelos@ars.usda.gov FU Swiss National Science Foundation [SNF PP00P2_133619] FX L.H.E.W. would like to acknowledge funding from the Swiss National Science Foundation (SNF PP00P2_133619). NR 288 TC 21 Z9 21 U1 30 U2 113 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD JUN PY 2015 VL 7 IS 6 BP 4199 EP 4239 DI 10.3390/nu7064199 PG 41 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CL9FN UT WOS:000357281800015 PM 26035246 ER PT J AU Gou, JY Li, K Wu, KT Wang, XD Lin, HQ Cantu, D Uauy, C Dobon-Alonso, A Midorikawa, T Inoue, K Sanchez, J Fu, DL Blechl, A Wallington, E Fahima, T Meeta, M Epstein, L Dubcovsky, J AF Gou, Jin-Ying Li, Kun Wu, Kati Wang, Xiaodong Lin, Huiqiong Cantu, Dario Uauy, Cristobal Dobon-Alonso, Albor Midorikawa, Takamufi Inoue, Kentaro Sanchez, Juan Fu, Daolin Blechl, Ann Wallington, Emma Fahima, Tzion Meeta, Madhu Epstein, Lynn Dubcovsky, Jorge TI Wheat Stripe Rust Resistance Protein WKS1 Reduces the Ability of the Thylakoid-Associated Ascorbate Peroxidase to Detoxify Reactive Oxygen Species SO PLANT CELL LA English DT Article ID F-SP TRITICI; PROGRAMMED CELL-DEATH; ENVELOPE MEMBRANE; DEFENSE RESPONSE; PLANT; ARABIDOPSIS; GENE; DISEASE; CHLOROPLASTS; TRANSPORT AB Stripe rust is a devastating fungal disease of wheat caused by Puccinia striiformis f. sp tritici (Pst). The WHEAT KINASE START1 (WKS1) resistance gene has an unusual combination of serine/threonine kinase and START lipid binding domains and confers partial resistance to Pst. Here, we show that wheat (Triticum aestivum) plants transformed with the complete WKS1 (variant WKS1.1) are resistant to Pst, whereas those transformed with an alternative splice variant with a truncated START domain (WKS1.2) are susceptible. WKS1.1 and WKS1.2 preferentially bind to the same lipids (phosphatidic acid and phosphatidylinositol phosphates) but differ in their protein-protein interactions. WKS1.1 is targeted to the chloroplast where it phosphorylates the thylakoid-associated ascorbate peroxidase (tAPX) and reduces its ability to detoxify peroxides. Increased expression of WKS1.1 in transgenic wheat accelerates leaf senescence in the absence of Pst. Based on these results, we propose that the phosphorylation of tAPX by WKS1.1 reduces the ability of the cells to detoxify reactive oxygen species and contributes to cell death. This response takes several days longer than typical hypersensitive cell death responses, thus allowing the limited pathogen growth and restricted sporulation that is characteristic of the WKS1 partial resistance response to Pst. C1 [Gou, Jin-Ying; Li, Kun; Wu, Kati; Wang, Xiaodong; Lin, Huiqiong; Midorikawa, Takamufi; Inoue, Kentaro; Sanchez, Juan; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Gou, Jin-Ying] Fudan Univ, Inst Plant Biol, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China. [Li, Kun; Fu, Daolin] Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China. [Cantu, Dario] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Uauy, Cristobal; Dobon-Alonso, Albor] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England. [Uauy, Cristobal; Wallington, Emma] Natl Inst Agr Bot, Cambridge CB3 0LE, England. [Blechl, Ann] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Fahima, Tzion] Univ Haifa, Inst Evolut, IL-31905 Haifa, Israel. [Fahima, Tzion] Univ Haifa, Dept Evolutionary & Environm Biol, IL-31905 Haifa, Israel. [Meeta, Madhu] Punjab Agr Univ, Dept Plant Breeding & Genet, Ludhiana 141004, Punjab, India. [Epstein, Lynn] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. RP Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM jdubcovsky@ucdavis.edu RI Cantu, Dario /E-7658-2010; Dubcovsky, Jorge/A-4969-2008; Uauy, Cristobal/B-7912-2014 OI Cantu, Dario /0000-0002-4858-1508; Dubcovsky, Jorge/0000-0002-7571-4345; Uauy, Cristobal/0000-0002-9814-1770 FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029]; US-Israel BARD Grants [US-4323-10C, IS-4628-13]; Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy [DE-FG02-08ER15963]; SCPRID Program from the Biotechnology and Biological Science Research Council [BB/J012017/1]; Howard Hughes Medical Institute; Gordon & Betty Moore Foundation; Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning [SHH1322012]; National Natural Science Foundation of China [31470386, 31110103917]; National Institute of Agricultural Botany Trust; Marie Curie fellowship FX This project was supported by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture, by US-Israel BARD Grants US-4323-10C and IS-4628-13, by Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy Grant DE-FG02-08ER15963, by the Major International Joint Research Project (31110103917) from the National Natural Science Foundation of China, and by the SCPRID Program (BB/J012017/1) from the Biotechnology and Biological Science Research Council. J.D. acknowledges the financial support of the Howard Hughes Medical Institute and Gordon & Betty Moore Foundation. J.-Y.G. is supported by The Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning (SHH1322012) and the National Natural Science Foundation of China (31470386). C.U. and E.W. acknowledge financial support from the National Institute of Agricultural Botany Trust and thank M. Craze and S. Bowden for transformation of wheat variety Glasgow as well as R. LeFevre and R. Howells for technical assistance. A.D.-A. is supported by a Marie Curie fellowship. The USDA is an equal opportunity provider and employer. Mention of a specific product name by the USDA does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 57 TC 13 Z9 14 U1 1 U2 27 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD JUN PY 2015 VL 27 IS 6 BP 1755 EP 1770 DI 10.1105/tpc.114.134296 PG 16 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA CM5HT UT WOS:000357718800016 PM 25991734 ER PT J AU Aubault, H Webb, NP Strong, CL McTainsh, GH Leys, JF Scanlan, JC AF Aubault, Helene Webb, Nicholas P. Strong, Craig L. McTainsh, Grant H. Leys, John F. Scanlan, Joe C. TI Grazing impacts on the susceptibility of rangelands to wind erosion: The effects of stocking rate, stocking strategy and land condition SO AEOLIAN RESEARCH LA English DT Article DE Aeolian; Dust; Soil erodibility; Rangeland management; Stocking rates; Land degradation ID BIOLOGICAL SOIL CRUSTS; RAINFALL VARIABILITY; NORTHERN AUSTRALIA; ARID RANGELAND; INNER-MONGOLIA; CLIMATE-CHANGE; CROP RESIDUES; DUST; MANAGEMENT; MODEL AB An estimated 110 Mt of dust is eroded by wind from the Australian land surface each year, most of which originates from the arid and semi-arid rangelands. Livestock production is thought to increase the susceptibility of the rangelands to wind erosion by reducing vegetation cover and modifying surface soil stability. However, research is yet to quantify the impacts of grazing land management on the erodibility of the Australian rangelands, or determine how these impacts vary among land types and over time. We present a simulation analysis that links a pasture growth and animal production model (GRASP) to the Australian Land Erodibility Model (AUSLEM) to evaluate the impacts of stocking rate, stocking strategy and land condition on the erodibility of four land types in western Queensland, Australia. Our results show that declining land condition, over stocking, and using inflexible stocking strategies have potential to increase land erodibility and amplify accelerated soil erosion. However, land erodibility responses to grazing are complex and influenced by land type sensitivities to different grazing strategies and local climate characteristics. Our simulations show that land types which are more resilient to livestock grazing tend to be least susceptible to accelerated wind erosion. Increases in land erodibility are found to occur most often during climatic transitions when vegetation cover is most sensitive to grazing pressure. However, grazing effects are limited during extreme wet and dry periods when the influence of climate on vegetation cover is strongest. Our research provides the opportunity to estimate the effects of different land management practices across a range of land types, and provides a better understanding of the mechanisms of accelerated erosion resulting from pastoral activities. The approach could help further assessment of land erodibility at a broader scale notably if combined with wind erosion models. (C) 2015 Elsevier B.V. All rights reserved. C1 [Aubault, Helene; McTainsh, Grant H.] Griffith Univ, Griffith Sch Environm, Brisbane, Qld 4111, Australia. [Webb, Nicholas P.] New Mexico State Univ, MSC JER 3, USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Strong, Craig L.] Australian Natl Univ, Fenner Sch Environm & Soc, Canberra, ACT 0200, Australia. [Leys, John F.] NSW Off Environm & Heritage, Sci Serv Div, Gunnedah, NSW 2380, Australia. [Scanlan, Joe C.] Dept Agr Fisheries & Forestry, Wilsonton, Qld 4350, Australia. RP Aubault, H (reprint author), Loughborough Univ Technol, Dept Geog, Loughborough LE11 3TU, Leics, England. EM h.a.p.aubault@lboro.ac.uk NR 78 TC 4 Z9 6 U1 8 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD JUN PY 2015 VL 17 BP 89 EP 99 DI 10.1016/j.aeolia.2014.12.005 PG 11 WC Geography, Physical SC Physical Geography GA CL7IW UT WOS:000357146700007 ER PT J AU Vallet, JL Miles, JR Rempel, LA Nonneman, DJ Lents, CA AF Vallet, J. L. Miles, J. R. Rempel, L. A. Nonneman, D. J. Lents, C. A. TI Relationships between day one piglet serum immunoglobulin immunocrit and subsequent growth, puberty attainment, litter size, and lactation performance SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT Forages and Pastures Symposium on Use of Marginal Lands and Fibrous Byproducts in Efficient Beef and Dairy Production Systems held at the Joint Annual Meeting of the Amer-Dairy-Sci-Assoc, the Amer-Soc-of Animal-Sci, and the Canadian-Soc-of-Animal-Sci CY JUL 20-24, 2014 CL Kansas City, MO SP Amer Dairy Sci Assoc, Amer Soc Animal Sci, Canadian Soc Animal Sci DE colostrum; growth; immunocrit; lactation; litter size; puberty ID NEONATAL PORCINE UTERUS; LACTOCRINE HYPOTHESIS; BIOLOGICAL-ACTIVITY; ENERGY-METABOLISM; PASSIVE TRANSFER; GENE-EXPRESSION; NEWBORN PIG; RELAXIN; MILK; MORTALITY AB Colostrum affects gut and uterine gland development in the neonatal piglet, suggesting that subsequent growth and reproductive performance may be affected. Measuring immunoglobulin in piglet serum using the immunoglobulin immunocrit on Day 1 of age provides a simple, inexpensive indication of the amount of colostrum acquired by the piglet in the first day of life. Relationships between serum immunoglobulin immunocrit measures and subsequent growth rates, age at puberty, incidence of puberty failure, litter size, and lactation performance were examined in pigs born and subsequently farrowing between 2009 and 2013. Immunoglobulin immunocrit measures were collected on 16,762 piglets on Day 1 of age. Of these piglets, BW measurements were available from 15,324 (7,684 males and 7,640 females) piglets at a range of ages from weaning to 200 d of age, allowing an assessment of growth rates. Age at puberty was recorded from a subset of 2,857 of the females after observing them for estrous behavior from approximately 170 to 250 d of age. To examine relationships between d 1 immunocrit and puberty failure, gilts with immunocrit measures that failed to reach puberty (n = 119) were matched with littermate gilts with immunocrit measures that achieved puberty (n = 167). Similarly, number born alive was collected on a subset (n = 799) of females from first to fourth parities for which d 1 immunocrits were measured on them as neonates. Finally, d 1 immunocrit effect on adult lactational competence was assessed by measuring litter average immunocrit (offspring of 440 females) and litter average piglet preweaning growth rate (offspring of 774 females) in females where d 1 immunocrits were available from them as neonates. Results indicated that low d 1 immunocrits were subsequently associated with reduced growth (P < 0.01), increased age at puberty (P < 0.01), reduced number born alive (P < 0.05), reduced litter average immunocrit (P < 0.05), and reduced litter average preweaning growth rate during lactation (P < 0.05). This suggests that management efforts to improve the amount of colostrum ingested by neonatal piglets would result in beneficial changes in production efficiency, particularly for gilts destined for the breeding herd. It also suggests that the immunoglobulin immunocrit can be useful in monitoring colostrum ingestion to maximize the beneficial effects of colostrum on subsequent performance. C1 [Vallet, J. L.; Miles, J. R.; Rempel, L. A.; Nonneman, D. J.; Lents, C. A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Vallet, JL (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM jeff.vallet@ars.usda.gov NR 34 TC 5 Z9 5 U1 1 U2 21 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2015 VL 93 IS 6 BP 2722 EP 2729 DI 10.2527/jas.2014-8535 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6VE UT WOS:000357104800013 PM 26115260 ER PT J AU Roever, CL DelCurto, T Rowland, M Vavra, M Wisdom, M AF Roever, C. L. DelCurto, T. Rowland, M. Vavra, M. Wisdom, M. TI Cattle grazing in semiarid forestlands: Habitat selection during periods of drought SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT Forages and Pastures Symposium on Use of Marginal Lands and Fibrous Byproducts in Efficient Beef and Dairy Production Systems held at the Joint Annual Meeting of the Amer-Dairy-Sci-Assoc, the Amer-Soc-of Animal-Sci, and the Canadian-Soc-of-Animal-Sci CY JUL 20-24, 2014 CL Kansas City, MO SP Amer Dairy Sci Assoc, Amer Soc Animal Sci, Canadian Soc Animal Sci DE biodiversity; Bos taurus; cattle; climate change; impacts; public land ID WESTERN UNITED-STATES; RESOURCE SELECTION; MANAGEMENT STRATEGIES; DISTRIBUTION PATTERNS; BLUE MOUNTAINS; GRIZZLY BEARS; RANGELANDS; LIVESTOCK; DIETS; WATER AB Climate change models are predicting increased frequency and severity of droughts in arid and semiarid environments, and these areas are responsible for much of the world's livestock production. Because cattle (Bos taurus) grazing can impact the abundance, distribution, and ecological function of native plant and animal communities, it is important to understand how cattle might respond to increasingly arid conditions. Here, we evaluate changes in habitat selection by cattle across an 8-yr period as a function of rainfall and other environmental covariates. Using resource selection functions, we evaluated habitat selection based on 2 behaviors, stationary and mobile. Models revealed similarity in cattle habitat selection across years, with only modest changes in selection as a function of precipitation, despite marked seasonal and interannual differences in rainfall. Cattle preferred gentle slopes, forest edges, wet meadows, and areas near water as well as areas far from water on plateaus. Cattle avoided areas at intermediate distances from water, typically associated with steep slopes. As conditions became drier during the late season, cattle did not switch selection patterns but instead contracted their selection around water. Cattle also selected similar habitats whether they were mobile or stationary, possibly making microsite decisions therein. This consistent pattern of selection across years could be particularly problematic for riparian communities as climates become drier; however, it may also simplify cattle management, as range managers can focus vegetation monitoring efforts on riparian areas. Due to the uncertainty surrounding future climatic conditions, it is imperative that both range and wildlife managers develop long-term plans to continue managing these multiuse landscapes in an ecologically sustainable manner based on expected patterns of livestock grazing. C1 [Roever, C. L.; DelCurto, T.] Oregon State Univ, Agr Program, La Grande, OR 97850 USA. [Rowland, M.; Vavra, M.; Wisdom, M.] USDA Forest Serv, Pacific Northwest Res Stn, La Grande, OR 97850 USA. RP Roever, CL (reprint author), Oregon State Univ, Agr Program, Badgley Hall 205,One Univ Blvd, La Grande, OR 97850 USA. EM mail@carrieroever.com FU USDA Forest Service Pacific Northwest Research Station; Oregon State University Agricultural and Natural Resources Research Center FX We thank Jennifer Hafer, Bridgett Naylor, and Cheryl Borum for GIS and data supported needed for our analyses and Pricilla Coe for expertise in interpreting collected data. Assistance with data analysis and methodology was provided by Hawthorne Beyer. Our work was funded by USDA Forest Service Pacific Northwest Research Station and Oregon State University Agricultural and Natural Resources Research Center. We would also like to thank the 2 anonymous reviewers for their thoughtful consideration and insightful comments to this manuscript. NR 66 TC 1 Z9 1 U1 3 U2 31 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2015 VL 93 IS 6 BP 3212 EP 3225 DI 10.2527/jas.2014-8794 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6VE UT WOS:000357104800060 PM 26115307 ER PT J AU Rogowski, A Briggs, JA Mortimer, JC Tryfona, T Terrapon, N Lowe, EC Basle, A Morland, C Day, AM Zheng, HJ Rogers, TE Thompson, P Hawkins, AR Yadav, MP Henrissat, B Martens, EC Dupree, P Gilbert, HJ Bolam, DN AF Rogowski, Artur Briggs, Jonathon A. Mortimer, Jennifer C. Tryfona, Theodora Terrapon, Nicolas Lowe, Elisabeth C. Basle, Arnaud Morland, Carl Day, Alison M. Zheng, Hongjun Rogers, Theresa E. Thompson, Paul Hawkins, Alastair R. Yadav, Madhav P. Henrissat, Bernard Martens, Eric C. Dupree, Paul Gilbert, Harry J. Bolam, David N. TI Glycan complexity dictates microbial resource allocation in the large intestine SO NATURE COMMUNICATIONS LA English DT Article ID HUMAN GUT MICROBIOTA; CARBOHYDRATE-BINDING MODULES; HYDROLASE FAMILY 30; CORN FIBER GUM; SUBSTRATE-SPECIFICITY; BACTEROIDES-THETAIOTAOMICRON; POLYSACCHARIDE UTILIZATION; BIFIDOBACTERIUM-BIFIDUM; ALPHA-GLUCURONIDASE; REACTION-MECHANISM AB The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota. C1 [Rogowski, Artur; Briggs, Jonathon A.; Lowe, Elisabeth C.; Basle, Arnaud; Morland, Carl; Day, Alison M.; Zheng, Hongjun; Thompson, Paul; Hawkins, Alastair R.; Gilbert, Harry J.; Bolam, David N.] Newcastle Univ, Sch Med, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England. [Mortimer, Jennifer C.; Tryfona, Theodora; Dupree, Paul] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England. [Terrapon, Nicolas; Henrissat, Bernard] Univ Aix Marseille, AFMB, UMR 7257, CNRS, F-13288 Marseille, France. [Rogers, Theresa E.; Martens, Eric C.] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA. [Yadav, Madhav P.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Henrissat, Bernard] King Abdulaziz Univ, Dept Biol Sci, Jeddah 21413, Saudi Arabia. RP Gilbert, HJ (reprint author), Newcastle Univ, Sch Med, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England. EM harry.gilbert@ncl.ac.uk; david.bolam@ncl.ac.uk RI Fac Sci, KAU, Biol Sci Dept/L-4228-2013; OI Rogers, Theresa/0000-0002-9741-5768; Mortimer, Jenny/0000-0001-6624-636X FU BBSRC [BB/G016186/1]; Wellcome Trust [WT097907AIA] FX We thank Dr Bruce Hamaker (Purdue University, IN, USA) for the kind gift of rice and sorghum xylans. This work was supported in part by grants to D.N.B. (BBSRC BB/G016186/1) and H.J.G. (Wellcome Trust WT097907AIA). NR 69 TC 28 Z9 28 U1 8 U2 37 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUN PY 2015 VL 6 AR 7481 DI 10.1038/ncomms8481 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL7UV UT WOS:000357178100001 PM 26112186 ER PT J AU Sheffield, CL Crippen, TL Byrd, JA Beier, RC Yeater, K AF Sheffield, Cynthia L. Crippen, Tawni L. Byrd, J. Allen Beier, Ross C. Yeater, Kathleen TI Canonical Discrimination of the Effect of a New Broiler Production Facility on Soil Chemical Profiles as Related to Current Management Practices SO PLOS ONE LA English DT Article ID GENETIC-VARIATION; PHOSPHORUS; EXTRACTANT; POTASSIUM; MANGANESE; NITROGEN; QUALITY; ZINC AB The effect dirt-floored broiler houses have on the underlying native soil, and the potential for contamination of the ground water by leaching under the foundation, is an understudied area. This study examines alterations in fifteen quantitative soil parameters (Ca, Cu, electrical conductivity, Fe, K, Mg, Mn, Na, NO3, organic matter, P, pH, S, soil moisture and Zn) in the underlayment of a newly constructed dirt-floored broiler house over the first two years of production (Native through Flock 11). The experiment was conducted near NW Robertson County, Texas, where the native soil is a fine, smectitic thermic Udertic Paleustalfs and the slopes range from zero to three percent. Multiple samples were collected from under each of three water and three feed lines the length of the house, in a longitudinal study during February 2008 through August 2010. To better define the relationship between the soil parameters and sampling times, a canonical discriminant analysis approach was used. The soil profiles assembled into five distinctive clusters corresponding to time and management practices. Results of this work revealed that the majority of parameters increased over time. The management practices of partial and total house clean-outs markedly altered soil profiles the house underlayment, thus reducing the risk of infiltration into the ground water near the farm. This is important as most broiler farms consist of several houses within a small area, so the cumulative ecological impact could be substantial if not properly managed. C1 [Sheffield, Cynthia L.; Crippen, Tawni L.; Byrd, J. Allen; Beier, Ross C.] ARS, USDA, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Yeater, Kathleen] ARS, USDA, Plains Area, Ft Collins, CO USA. RP Sheffield, CL (reprint author), ARS, USDA, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. EM cindy.sheffield@ars.usda.gov FU United States Department of Agriculture FX The authors extend their thanks to our commercial partner for allowing us to work in cooperation with their producers. We also gratefully recognize Andrew Herndon, Denise Caldwell, Charles Hernandez, Laura Ripley and Bob Droleskey for their invaluable help in both the field and laboratory. This project was funded by the United States Department of Agriculture. There is no commercial or financial conflict of interest. Mention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may be suitable. NR 29 TC 0 Z9 0 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 1 PY 2015 VL 10 IS 6 AR e0128179 DI 10.1371/journal.pone.0128179 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CL0KF UT WOS:000356630900120 PM 26029909 ER PT J AU Muharam, FM Maas, SJ Bronson, KF Delahunty, T AF Muharam, Farrah Melissa Maas, Stephen J. Bronson, Kevin F. Delahunty, Tina TI Estimating Cotton Nitrogen Nutrition Status Using Leaf Greenness and Ground Cover Information SO REMOTE SENSING LA English DT Article ID DIFFERENCE VEGETATION INDEX; HIGHER-PLANT LEAVES; CHLOROPHYLL CONTENT; PRECISION AGRICULTURE; SPECTRAL REFLECTANCE; IRRIGATED COTTON; CANOPY SCALES; FRACTION; ALGORITHMS; INDICATORS AB Assessing nitrogen (N) status is important from economic and environmental standpoints. To date, many spectral indices to estimate cotton chlorophyll or N content have been purely developed using statistical analysis approach where they are often subject to site-specific problems. This study describes and tests a novel method of utilizing physical characteristics of N-fertilized cotton and combining field spectral measurements made at different spatial scales as an approach to estimate in-season chlorophyll or leaf N content of field-grown cotton. In this study, leaf greenness estimated from spectral measurements made at the individual leaf, canopy and scene levels was combined with percent ground cover to produce three different indices, named TCCLeaf, TCCCanopy, and TCCScene. These indices worked best for estimating leaf N at early flowering, but not for chlorophyll content. Of the three indices, TCCLeaf showed the best ability to estimate leaf N (R-2 = 0.89). These results suggest that the use of green and red-edge wavelengths derived at the leaf scale is best for estimating leaf greenness. TCCCanopy had a slightly lower R-2 value than TCCLeaf (0.76), suggesting that the utilization of yellow and red-edge wavelengths obtained at the canopy level could be used as an alternative to estimate leaf N in the absence of leaf spectral information. The relationship between TCCScene and leaf N was the lowest (R-2 = 0.50), indicating that the estimation of canopy greenness from scene measurements needs improvement. Results from this study confirmed the potential of these indices as efficient methods for estimating in-season leaf N status of cotton. C1 [Muharam, Farrah Melissa; Maas, Stephen J.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. [Muharam, Farrah Melissa] Univ Putra Malaysia, Fac Agr, Dept Agr Technol, Upm Serdang 43400, Selangor, Malaysia. [Bronson, Kevin F.] ARS, US Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. [Delahunty, Tina] Bloomsburg Univ Penn, Dept Environm Geog & Geol Sci, Bloomsburg, PA 17815 USA. RP Muharam, FM (reprint author), Texas Tech Univ, Dept Plant & Soil Sci, MS42122, Lubbock, TX 79409 USA. EM farrahm@upm.edu.my; stephen.maas@ttu.edu; Kevin.Bronson@ars.usda.gov; tdelahun@bloomu.edu FU Universiti Putra Malaysia; Ministry of Education, Malaysia FX The main author would like to thank Universiti Putra Malaysia and Ministry of Education, Malaysia for study leave and scholarship. NR 47 TC 0 Z9 0 U1 5 U2 16 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUN PY 2015 VL 7 IS 6 BP 7007 EP 7028 DI 10.3390/rs70607007 PG 22 WC Remote Sensing SC Remote Sensing GA CM3NS UT WOS:000357589800021 ER PT J AU Young, JK Miller, E Essex, A AF Young, Julie K. Miller, Elizabeth Essex, Anna TI Evaluating Fladry Designs to Improve Utility as a Nonlethal Management Tool to Reduce Livestock Depredation SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE carnivore conservation; livestock depredation; nonlethal control; predator management ID GUARDING DOGS; WOLVES; WOLF; CONSERVATION; ATTITUDES; REINTRODUCTION; POPULATIONS; PROTECTION; EDGE AB Nonlethal deterrents against carnivores are important components to protecting livestock and conserving carnivore populations. However, the performance of the visual deterrent called fladry, a historical tool used to defend livestock from carnivores, is often hindered by design flaws that eventually reduce its effectiveness. Our purpose was to identify a fladry design that reduces coiling (i.e., wrapping of individual flags tight to the rope from which they hang) and maintains free movement of the deterrent in the wind. We created 6 new designs, replicated designs using 2 materials (nylon and marine vinyl), and compared them with the design most commonly used today-where flags were sewn directly onto the line along which they are strung. We conducted the study during January-February 2014 at the U.S. Department of Agriculture, Wildlife Services, Predator Research Facility in Millville, Utah, USA. Fladry made of marine vinyl and attached via 2 of our 6 designs showed the least amount of coiling, were relatively easy to construct, and did not result in significant additional costs. The 2 designs were shower curtain, where the flags are attached via circular links, and knotted, where a knot is tied in the flag below its point of attachment. We suggest users of nylon fladry modify it to one of these designs and advise new users to consider a heavier (e.g., marine vinyl) material. (C) 2015 The Wildlife Society. C1 [Young, Julie K.; Miller, Elizabeth] USDA, Natl Wildlife Res Ctr, Predator Res Field Stn, Logan, UT 84322 USA. [Essex, Anna] Ecole Natl Super Agron Toulouse, Inst Natl Polytech, Toulouse, France. RP Young, JK (reprint author), USDA, Natl Wildlife Res Ctr, Predator Res Field Stn, USU BNR 163, Logan, UT 84322 USA. EM julie.k.young@aphis.usda.gov FU U.S. Department of Agriculture-National Wildlife Research Center FX We thank A. Roadman and K. Schenavar for help with design and sewing of fladry. S. Brummer, E. Stevenson, G. Labadie, and J. Jouffrey helped with setting up fladry. S. Brummer also aided in data collection. We are grateful to M. Conner for statistical assistance with study design and analysis in Program MARK. We thank H. Weaver, 2 anonymous reviewers, and the Associate Editor for their comments. This project was funded by U.S. Department of Agriculture-National Wildlife Research Center. NR 26 TC 0 Z9 0 U1 3 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD JUN PY 2015 VL 39 IS 2 BP 429 EP 433 DI 10.1002/wsb.531 PG 5 WC Biodiversity Conservation SC Biodiversity & Conservation GA CL3VR UT WOS:000356879900025 ER PT J AU Swanepoel, PA Sanderson, MA Bell, LW Thomas, DT Bennett, JE AF Swanepoel, Pieter A. Sanderson, Matt A. Bell, Lindsay W. Thomas, Dean T. Bennett, James E. TI Southern African pasture science in the 21st century SO AFRICAN JOURNAL OF RANGE & FORAGE SCIENCE LA English DT Editorial Material ID KIKUYU OVER-SOWN; CULTIVATED PASTURES; PERENNIAL RYEGRASS; QUALITY C1 [Swanepoel, Pieter A.] Univ Stellenbosch, Dept Agron, ZA-7600 Stellenbosch, South Africa. [Sanderson, Matt A.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Bell, Lindsay W.] CSIRO, Sustainable Agr Flagship, Toowoomba, Qld, Australia. [Thomas, Dean T.] CSIRO, Sustainable Agr Flagship, Wembley, WA, Australia. [Bennett, James E.] Coventry Univ, Dept Geog Environm & Disaster Management, Coventry, W Midlands, England. [Bennett, James E.] Coventry Univ, Ctr Agroecol Water & Resilience, Coventry, W Midlands, England. RP Swanepoel, PA (reprint author), Univ Stellenbosch, Dept Agron, ZA-7600 Stellenbosch, South Africa. EM pieterswanepoel@sun.ac.za RI Bell, Lindsay/B-9769-2008; Swanepoel, Pieter/L-2536-2015; Thomas, Dean/E-4096-2010; OI Swanepoel, Pieter/0000-0002-6481-0673; Thomas, Dean/0000-0001-6653-2315; Bennett, James/0000-0002-7233-6203 NR 11 TC 0 Z9 0 U1 1 U2 4 PU NATL INQUIRY SERVICES CENTRE PTY LTD PI GRAHAMSTOWN PA 19 WORCESTER STREET, PO BOX 377, GRAHAMSTOWN 6140, SOUTH AFRICA SN 1022-0119 EI 1727-9380 J9 AFR J RANGE FOR SCI JI Afr. J. Range Forage Sci. PD JUN PY 2015 VL 32 IS 2 SI SI DI 10.2989/10220119.2015.1060693 PG 2 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CL4PO UT WOS:000356935600001 ER PT J AU Gibson, BW Boles, NC Souroullas, GP Herron, AJ Fraley, JK Schwiebert, RS Sharp, JJ Goodell, MA AF Gibson, Brian W. Boles, Nathan C. Souroullas, George P. Herron, Alan J. Fraley, Joe K. Schwiebert, Rebecca S. Sharp, John J. Goodell, Margaret A. TI Comparison of Cesium-137 and X-ray Irradiators by Using Bone Marrow Transplant Reconstitution in C57BL/6J Mice SO COMPARATIVE MEDICINE LA English DT Article AB Mice are used extensively in transplantation studies involving bone marrow ablation. Due to the increasing security issues and expenses involved with gamma irradiators, self-contained X-ray irradiators have been increasing in popularity. We hypothesized that bone marrow ablation by irradiation of mice with a Cs-137 irradiator would be comparable to that from an X-ray source irradiator. A lethaldose curve was obtained by irradiating C57BL/6J mice with 500, 700, 900, and 1100 cGy from either source. These data were used to determine the lethal radiation exposure range for a noncompetitive bone marrow engraftment curve for each source. At 90 d after reconstitution, the bone marrow engraftment curves revealed significant differences between the 2 sources in the establishment of B cell, myeloid, and T cell lineages. Murine B cell reconstitution after exposure to a Cs-137 source was greater than that after X-ray exposure at each dose level, whereas the converse was true for myeloid cell reconstitution. At the 1050- and 1100-cGy doses, mice irradiated by using the X-ray source demonstrated higher levels of T cell reconstitution but decreased survival compared with mice irradiated with the Cs-137 source. We concluded that although both sources ablated endogenous bone marrow sufficiently to enable stem cell engraftment, there are distinct physiologic responses that should be considered when choosing the optimal source for use in a study and that irradiation from the Cs-137 source was associated with lower overall morbidity due to opportunistic infection. C1 [Gibson, Brian W.; Schwiebert, Rebecca S.; Sharp, John J.] Baylor Coll Med, Ctr Comparat Med, USDA ARS, Childrens Res Ctr, Houston, TX 77030 USA. [Boles, Nathan C.; Souroullas, George P.; Goodell, Margaret A.] Baylor Coll Med, Dept Pediat & Mol & Human Genet, USDA ARS, Childrens Res Ctr, Houston, TX 77030 USA. [Herron, Alan J.] Baylor Coll Med, Dept Pathol & Immunol, USDA ARS, Childrens Res Ctr, Houston, TX 77030 USA. [Fraley, Joe K.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Res Ctr, Houston, TX 77030 USA. RP Gibson, BW (reprint author), Baylor Coll Med, Ctr Comparat Med, USDA ARS, Childrens Res Ctr, Houston, TX 77030 USA. EM bwg@bcm.tmc.edu OI Goodell, Margaret/0000-0003-1111-2932 FU NIH [DK092883, NCRR S10RR024574, NIAID AI036211, NCI P30CA125123]; BCM Cytometry and Cell Sorting Core FX The authors thank Bilques Bhatti and her histopathology staff for their many hours of tissue processing and histopathology slide preparation. We extend our gratitude to Stephanie Barrett, Benedict Gabriel, Francisco Crisanto, Jorge De La Rosa, Darshica Simon, Kenneth Thompson, Jennifer Volkmann, Miguel Posadas, and Marta Hernandez for their husbandry support during this project. We thank Scott Buss for his photographic contributions. This project was supported by the NIH (grant no. DK092883) and the BCM Cytometry and Cell Sorting Core with funding from the NIH (NCRR S10RR024574, NIAID AI036211, and NCI P30CA125123). NR 17 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC LABORATORY ANIMAL SCIENCE PI MEMPHIS PA 9190 CRESTWYN HILLS DR, MEMPHIS, TN 38125 USA SN 1532-0820 J9 COMPARATIVE MED JI Comparative Med. PD JUN PY 2015 VL 65 IS 3 BP 165 EP 172 PG 8 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA CL7JA UT WOS:000357147100002 PM 26141441 ER PT J AU Naidu, R Channey, R McConnell, S Johnston, N Semple, KT McGrath, S Dries, V Nathanail, P Harmsen, J Pruszinski, A MacMillan, J Palanisami, T AF Naidu, Ravi Channey, Rufus McConnell, Stuart Johnston, Niall Semple, Kirk T. McGrath, Steve Dries, Victor Nathanail, Paul Harmsen, Joop Pruszinski, Andrew MacMillan, Janet Palanisami, Thavamani TI Towards bioavailability-based soil criteria: past, present and future perspectives SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article; Proceedings Paper CT 6th International Workshop on Chemical Bioavailability in the Terrestrial Environment CY SEP 11-15, 2011 CL Adelaide, AUSTRALIA DE Bioavailability; Bioaccessibility; Risk assessment; Site assessment; Regulators; Remediation AB Bioavailability has been used as a key indicator in chemical risk assessment yet poorly quantified risk factor. Worldwide, the framework used to assess potentially contaminated sites is similar, and the decisions are based on threshold contaminant concentration. The uncertainty in the definition and measurement of bioavailability had limited its application to environment risk assessment and remediation. Last ten years have seen major developments in bioavailability research and acceptance. The use of bioavailability in the decision making process as one of the key variables has led to a gradual shift towards a more sophisticated risk-based approach. Now a days, many decision makers and regulatory organisations 'more readily accept' this concept. Bioavailability should be the underlying basis for risk assessment and setting remediation goals of those contaminated sites that pose risk to environmental and human health. This paper summarises the potential application of contaminant bioavailability and bioaccessibility to the assessment of sites affected by different contaminants, and the potential for this to be the underlying basis for sustainable risk assessment and remediation in Europe, North America and Australia over the coming decade. C1 [Naidu, Ravi; Palanisami, Thavamani] Univ S Australia, Cooperat Res Ctr Contaminat Assessment & Remediat, Salisbury, SA 5095, Australia. [Channey, Rufus] ARS, USDA, Environm Management & By Prod Utilizat Lab, Beltsville, MD 20705 USA. [McConnell, Stuart] EPA, Melbourne, Vic, Australia. [Johnston, Niall] DECC, Sydney, NSW, Australia. [Semple, Kirk T.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [McGrath, Steve] Rothamsted Res, West Common, Herts, England. [Dries, Victor] Publ Waste Agcy Flanders OVAM, B-2800 Mechelen, Belgium. [Nathanail, Paul] Univ Nottingham, Nottingham NG7 2RD, England. [Nathanail, Paul] Land Qual Management Ltd, Nottingham NG7 2RD, England. [Harmsen, Joop] Alterra, Wageningen UR, NL-6700 AA Wageningen, Netherlands. [Pruszinski, Andrew] EPA, Adelaide, SA, Australia. [MacMillan, Janet] Western Australian Dept Environm & Conservat, Perth, WA, Australia. RP Naidu, R (reprint author), Univ S Australia, Cooperat Res Ctr Contaminat Assessment & Remediat, Bldg 10, Salisbury, SA 5095, Australia. EM Ravi.Naidu@crccare.com RI Semple, Kirk/H-4466-2013; OI Semple, Kirk/0000-0002-4046-2037; McGrath, Stephen/0000-0003-0952-8947; Nathanail, C Paul/0000-0001-6200-3985 NR 11 TC 5 Z9 5 U1 5 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD JUN PY 2015 VL 22 IS 12 BP 8779 EP 8785 DI 10.1007/s11356-013-1617-x PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CK7YL UT WOS:000356453000002 PM 23519481 ER PT J AU Ben M'Barek, S Cordewener, JHG Ghaffary, SMT van der Lee, TAJ Liu, ZH Gohari, AM Mehrabi, R America, AHP Robert, O Friesen, TL Hamza, S Stergiopoulos, I de Wit, PJGM Kema, GHJ AF Ben M'Barek, Sarrah Cordewener, Jan H. G. Ghaffary, Seyed M. Tabib van der Lee, Theo A. J. Liu, Zhaohui Gohari, Amir. Mirzadi Mehrabi, Rahim America, Antoine H. P. Robert, Olivier Friesen, Timothy L. Hamza, Sonia Stergiopoulos, Ioannis de Wit, Pierre J. G. M. Kema, Gerrit H. J. TI FPLC and liquid-chromatography mass spectrometry identify candidate necrosis-inducing proteins from culture filtrates of the fungal wheat pathogen Zymoseptoria tritici SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Zymoseptoria tritici; Culture filtrates; Fast protein liquid chromatography; Liquid chromatography mass spectrometry; Necrosis-inducing proteins ID HOST-SELECTIVE TOXINS; MYCOSPHAERELLA-GRAMINICOLA; STAGONOSPORA-NODORUM; SEPTORIA-TRITICI; RHYNCHOSPORIUM-SECALIS; CLADOSPORIUM-FULVUM; PTR TOXA; HYDROGEN-PEROXIDE; BARLEY PATHOGEN; TAN SPOT AB Culture filtrates (CFs) of the fungal wheat pathogen Zymoseptoria tritici were assayed for necrosis-inducing activity after infiltration in leaves of various wheat cultivars. Active fractions were partially purified and characterized. The necrosis-inducing factors in CFs are proteinaceous, heat stable and their necrosis-inducing activity is temperature and light dependent. The in planta activity of CFs was tested by a time series of proteinase K (PK) co-infiltrations, which was unable to affect activity 30 min after CF infiltrations. This suggests that the necrosis inducing proteins (NIPs) are either absent from the apoplast and likely actively transported into mesophyll cells or protected from the protease by association with a receptor. Alternatively, plant cell death signaling pathways might be fully engaged during the first 30 min and cannot be reversed even after PK treatment. Further fractionation of the CFs with the highest necrosis-inducing activity involved fast performance liquid chromatography, SDS-PAGE and mass spectrometry. This revealed that most of the proteins present in the fractions have not been described before. The two most prominent ZtNIP encoding candidates were heterologously expressed in Pichia pastoris and subsequent infiltration assays showed their differential activity in a range of wheat cultivars. (C) 2015 Elsevier Inc. All rights reserved. C1 [Ben M'Barek, Sarrah; Cordewener, Jan H. G.; Ghaffary, Seyed M. Tabib; van der Lee, Theo A. J.; Gohari, Amir. Mirzadi; America, Antoine H. P.; Kema, Gerrit H. J.] Wageningen Univ & Res Ctr, Plant Res Int, NL-6708 PB Wageningen, Netherlands. [Ben M'Barek, Sarrah] Ctr Biotechnol Borj Cedria, Lab Plant Mol Physiol, Hammam Lif 2050, Tunisia. [Cordewener, Jan H. G.; America, Antoine H. P.] Ctr BioSyst & Genom, NL-6700 AA Wageningen, Netherlands. [Cordewener, Jan H. G.; America, Antoine H. P.] Netherlands Prote Ctr, Utrecht, Netherlands. [Ghaffary, Seyed M. Tabib] Safiabad Agr & Nat Resource Res Ctr, Dezfoul, Iran. [Liu, Zhaohui] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Gohari, Amir. Mirzadi] Univ Tehran, Coll Agr, Dept Plant Protect, Karaj, Iran. [Mehrabi, Rahim] Seed & Plant Improvement Inst, Cereal Res Dept, Karaj, Iran. [Robert, Olivier] Florimond Desprez, F-59242 Cappelle En Pevele, France. [Friesen, Timothy L.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Hamza, Sonia] Natl Agron Inst Tunisia, Genet Lab, Tunis 1082, Tunisia. [Stergiopoulos, Ioannis] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [de Wit, Pierre J. G. M.] Wageningen Univ, Lab Phytopathol, NL-6708 PB Wageningen, Netherlands. RP Kema, GHJ (reprint author), Wageningen Univ & Res Ctr, Plant Res Int BV, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. EM gert.kema@wur.nl OI Mehrabi, Rahim/0000-0002-5098-9123 FU UNESCO L'Oreal fellowship FX The authors thank the members of the International Mycosphaerella Genomics Consortium for discussions and suggestions. We thankfully remember the late Joost de Groot and his kind provision of the FastaFileMerge bioinformatics tool. We also thank Els Verstappen for excellent Z. tritici maintenance and greenhouse management and Ineke de Vries for technical assistance. Sarrah Ben M'Barek was sponsored by an UNESCO L'Oreal fellowship. NR 68 TC 3 Z9 3 U1 4 U2 22 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUN PY 2015 VL 79 SI SI BP 54 EP 62 DI 10.1016/j.fgb.2015.03.015 PG 9 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA CL5IP UT WOS:000356993500010 PM 26092790 ER PT J AU Wright, MS AF Wright, Maureen S. TI Preliminary observations of sugarcane trash degradation for repurposing as mulch SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE sugarcane; leaves; trash; mulch; compost ID SEASONAL-VARIATIONS; EXTRANEOUS MATTER; RESIDUE; STALK; MANAGEMENT; QUALITY; CANE AB A potential use for sugarcane trash is conversion to mulch. This study evaluated whether a compost enhancer or nitrogen would accelerate degradation. Trash was obtained from a sugarcane grower, and treated with water only, a compost starter, and/or nitrogen at rates of 1 kg (Low) or 2 kg (High) / 10 m(2). Treatments were monitored for 21 weeks. Height of the trash, temperature and visual changes were determined. Bins that received water only had the greatest reduction in height, 54.5%. None of the treatments resulted in a change in temperature, possibly due to the mass of material in each bin. All watered bins had a greater observed change in consistency, and the low nitrogen treatment changed in color and texture in all replicates. Intact bales were also observed. Trash in the center of bales that were watered and left dry underwent changes in color and texture. While dry bales did not receive added water, they likely held sufficient moisture from rainfall allowing for conversion of the leaves. Added water was necessary for conversion of trash in the bins, and enhanced by the addition of low nitrogen. Larger masses of trash would allow temperature maintenance to potentially accelerate conversion in future studies. C1 USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Wright, MS (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Maureen.Wright@ARS.USDA.GOV NR 22 TC 0 Z9 0 U1 0 U2 0 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD JUN PY 2015 VL 117 IS 1398 BP 434 EP 439 PG 6 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA CL4GL UT WOS:000356910500032 ER PT J AU Dunbar, J Allen, P White, J Neupane, R Xu, T Wolfe, J Arnold, J AF Dunbar, John Allen, Peter White, Joseph Neupane, Ram Xu, Tian Wolfe, June Arnold, Jeff TI Characterizing a Shallow Groundwater System beneath Irrigated Sugarcane with Electrical Resistivity and Radon (Rn-222) Puunene, Hawaii SO JOURNAL OF ENVIRONMENTAL AND ENGINEERING GEOPHYSICS LA English DT Article ID CANAL SEEPAGE; WATER-CONTENT; TOMOGRAPHY; RECHARGE; TRACERS; STREAMS; BASALT; FLOW; USA AB In this study, we use a combination of electrical resistivity profiling and radon (Rn-222) measurements to characterize a shallow groundwater system beneath the last remaining, large-scale sugarcane plantation on Maui, Hawaii. Hawaiian Commercial & Sugar Company has continuously operated a sugarcane plantation on the western flank of Haleakala Volcano since 1878. The sugarcane is irrigated with a combination of surface water brought through tunnels from the wetter, eastern flank of Haleakala Volcano and groundwater from wells within the plantation. To assess the flow of irrigation water through the shallow subsurface, we collected a representative topo-sequence of four 2-D resistivity profiles that sample different topographic and hydrologic settings within the plantation. The profiles show a down-slope-thickening (0 to 20 m), surficial low-resistivity (10-200 Ohm-m) layer extending from the upslope limit of irrigated sugarcane to the lowest elevations of the plantation. At a canal crossing, the low resistivity layer thickens and is less resistive upslope of the canal. Beneath a reservoir at mid-elevation, the layer thickens to 20 m and curves down slope beneath the reservoir and up to the base of the field beyond. At the base of the slope, the low resistivity layer is 20-m thick below both fields and a second reservoir. An increase in radon concentration in the down-flow direction within the canal system at one location suggests groundwater infiltration into the canal. We attribute the low-resistivity layer to irrigation water that has infiltrated below the root zone and leaked from canals and reservoirs within the plantation. The water flows down slope to the base of the slope and there flows vertically, recharging the basal aquifer. We suggest that seepage from the canals and reservoirs is in part controlled by the local pressure head within the shallow flow system. C1 [Dunbar, John; Allen, Peter; Xu, Tian] Baylor Univ, Dept Geol, Waco, TX 76798 USA. [White, Joseph; Neupane, Ram] Baylor Univ, Dept Biol, Waco, TX 76798 USA. [Wolfe, June] Texas A&M Univ, AgriLife Res & Extens Ctr, Temple, TX 76502 USA. [Arnold, Jeff] USDA ARS, Temple, TX 76502 USA. RP Dunbar, J (reprint author), Baylor Univ, Dept Geol, One Bear Pl 97354, Waco, TX 76798 USA. EM john_dunbar@baylor.edu FU USDA [32110119] FX This project was supported by USDA Grant 32110119. We are thankful for logistical help and guidance from Mae Nakahata and Rick Volner, of HC&S. NR 57 TC 0 Z9 0 U1 2 U2 6 PU ENVIRONMENTAL ENGINEERING GEOPHYSICAL SOC PI DENVER PA 1720 SOUTH BELLAIRE, STE 110, DENVER, CO 80222-433 USA SN 1083-1363 J9 J ENVIRON ENG GEOPH JI J. Environ. Eng. Geophys. PD JUN PY 2015 VL 20 IS 2 BP 165 EP 181 DI 10.2113/JEEG20.2.165 PG 17 WC Geochemistry & Geophysics; Engineering, Geological SC Geochemistry & Geophysics; Engineering GA CL4GQ UT WOS:000356911000005 ER PT J AU Sun, G Michelsen, AM Sheng, ZP Fang, AF Shang, YZ Zhang, HL AF Sun, Ge Michelsen, Ari M. Sheng, Zhuping Fang, Andrew Feng Shang, Yizi Zhang, Huilan TI Featured Collection Introduction: Water for Megacities Challenges and Solutions SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article C1 [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Michelsen, Ari M.] Texas A&M AgriLife Res Ctr El Paso, Dept Agr Econ, El Paso, TX 79927 USA. [Sheng, Zhuping] Texas A&M AgriLife Res Ctr El Paso, Dept Biol & Agr Engn, El Paso, TX 79927 USA. [Fang, Andrew Feng] Kieser & Associates LLC, Kalamazoo, MI 49007 USA. [Shang, Yizi] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing 100038, Peoples R China. [Zhang, Huilan] Beijing Forestry Univ, Minist Educ, Key Lab Soil & Water Conservat & Desertificat Com, Beijing 100083, Peoples R China. RP Sun, G (reprint author), US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, 920 Main Campus Dr,Venture Ctr 2,Suite 300, Raleigh, NC 27606 USA. EM gesun@fs.fed.us OI shang, Yizi/0000-0002-8432-9561 NR 18 TC 2 Z9 2 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2015 VL 51 IS 3 BP 585 EP 588 DI 10.1111/1752-1688.12317 PG 4 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CL2YO UT WOS:000356814300001 ER PT J AU Hao, L Sun, G Liu, YQ Qian, H AF Hao, Lu Sun, Ge Liu, Yongqiang Qian, Hong TI Integrated Modeling of Water Supply and Demand under Management Options and Climate Change Scenarios in Chifeng City, China SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE climate change; hydrological modeling; water demand and supply; integrated water management ID GENETIC SOIL CLASSIFICATION; LAND-USE; UNITED-STATES; RIVER-BASIN; IMPACTS; IRRIGATION; CALIFORNIA; PROJECTIONS; RESOURCES; DEPLETION AB Water resource management is becoming increasingly challenging in northern China because of the rapid increase in water demand and decline in water supply due to climate change. We provide a case study demonstrating the importance of integrated watershed management in sustaining water resources in Chifeng City, northern China. We examine the consequences of various climate change scenarios and adaptive management options on water supply by integrating the Soil and Water Assessment Tool and Water Evaluation and Planning models. We show how integrated modeling is useful in projecting the likely effects of management options using limited information. Our study indicates that constructing more reservoirs can alleviate the current water shortage and groundwater depletion problems. However, this option is not necessarily the most effective measure to solve water supply problems; instead, improving irrigation efficiency and changing cropping structure may be more effective. Furthermore, measures to increase water supply have limited effects on water availability under a continuous drought and a dry-and-warm climate scenario. We conclude that the combined measure of reducing water demand and increasing supply is the most effective and practical solution for the water shortage problems in the study area. C1 [Hao, Lu] Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Int Ctr Ecol Meteorol & Environm, Nanjing 210044, Jiangsu, Peoples R China. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, USDA, Athens, GA 30602 USA. [Qian, Hong] Nanjing Univ Informat Sci & Technol, Sch Appl Meteorol, Int Ctr Ecol Meteorol & Environm, Nanjing 210044, Jiangsu, Peoples R China. RP Hao, L (reprint author), Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Int Ctr Ecol Meteorol & Environm, 219 Ningliu Rd, Nanjing 210044, Jiangsu, Peoples R China. EM hl_haolu@163.com FU Natural Science Foundation of China (NSFC) [71373130, 91425301]; Jiangsu Key Laboratory of Agricultural Meteorology Fund [KYQ1201]; Chinese Special Fund for Meteorological-Scientific Research in the Public Interest [GYHY 201206023]; National Key Basic Research Program of China [2013CB430200, 2013CB430206]; Southern Research Station, USDA Forest Service FX This work was supported by the Natural Science Foundation of China (NSFC, no. 71373130 and no. 91425301), the Jiangsu Key Laboratory of Agricultural Meteorology Fund (no. KYQ1201), the Chinese Special Fund for Meteorological-Scientific Research in the Public Interest (no. GYHY 201206023), and the National Key Basic Research Program of China (2013CB430200, 2013CB430206). We thank the China Meteorological Administration and Environmental & Ecological Science Data Center for West China, NSFC, and Institute of Soil Science of the Chinese Academy of Sciences for their assistance in data sharing. The WEAP model was provided by the Stockholm Environment Institute (SEI). Partial support was also received from the Southern Research Station, USDA Forest Service. We also thank three anonymous reviewers and the Guest Editor Dr. Huilan Zhang for valuable comments and suggestions that improved the earlier version of the manuscript, and Lisa Delp Taylor for English editing. NR 50 TC 5 Z9 5 U1 2 U2 16 PU WILEY PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2015 VL 51 IS 3 BP 655 EP 671 DI 10.1111/1752-1688.12311 PG 17 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CL2YO UT WOS:000356814300006 ER PT J AU White, M Harmel, D Yen, H Arnold, J Gambone, M Haney, R AF White, Michael Harmel, Daren Yen, Haw Arnold, Jeff Gambone, Marilyn Haney, Richard TI Development of Sediment and Nutrient Export Coefficients for US Ecoregions SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE sediment; nutrients; surface water hydrology; simulation; nonpoint source pollution ID UNITED-STATES; RIVER-BASIN; LAND USES; MANAGEMENT; LOAD; NITROGEN; DATABASE; RUNOFF; YIELDS; LAKES AB Water quality impairment due to excessive nutrients and sediment is a major problem in the United States (U.S.). An important step in the mitigation of impairment in any given water body is determination of pollutant sources and amount. The sheer number of impaired waters and limited resources makes simplistic load estimation methods such as export coefficient (EC) methods attractive. Unfortunately ECs are typically based on small watershed monitoring data, which are very limited and/or often based on data collected from distant watersheds with drastically different conditions. In this research, we seek to improve the accuracy of these nutrient export estimation methods by developing a national database of localized EC for each ecoregion in the U.S. A stochastic sampling methodology loosely based on the Monte-Carlo technique was used to construct a database of 45million Soil and Water Assessment Tool (SWAT) simulations. These simulations consider a variety of climate, topography, soils, weather, land use, management, and conservation implementation conditions. SWAT model simulations were successfully validated with edge-of-field monitoring data. Simulated nutrient ECs compared favorably with previously published studies. These ECs may be used to rapidly estimate nutrient loading for any small catchment in the U.S. provided the location, area, and land-use distribution are known. C1 [White, Michael; Harmel, Daren; Arnold, Jeff; Haney, Richard] ARS, USDA, Temple, TX 76502 USA. [Yen, Haw; Gambone, Marilyn] Texas A&M AgriLife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. RP White, M (reprint author), ARS, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. EM mike.white@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service FX This work was funded by the U.S. Department of Agriculture, Agricultural Research Service. USDA is an equal opportunity provider and employer. NR 34 TC 6 Z9 6 U1 4 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2015 VL 51 IS 3 BP 758 EP 775 DI 10.1111/jawr.12270 PG 18 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CL2YO UT WOS:000356814300013 ER PT J AU Moorhead, JE Gowda, PH Singh, VP Porter, DO Marek, TH Howell, TA Stewart, BA AF Moorhead, Jerry E. Gowda, Prasanna H. Singh, Vijay P. Porter, Dana O. Marek, Thomas H. Howell, Terry A. Stewart, B. A. TI Identifying and Evaluating a Suitable Index for Agricultural Drought Monitoring in the Texas High Plains SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE evapotranspiration; Standardized Precipitation Index; Standardized Precipitation-Evapotranspiration Index; semiarid regions ID SEVERITY INDEX; UNITED-STATES; EVAPOTRANSPIRATION AB Drought is a complex and highly destructive natural phenomenon that affects portions of the United States almost every year, and severe water deficiencies can often become catastrophic for agricultural production. Evapotranspiration (ET) by crops is an important component in the agricultural water budget; thus, it is advantageous to include ET in agricultural drought monitoring. The main objectives of this study were to (1) conduct a literature review of drought indices with a focus to identify a simple but simultaneously adequate drought index for monitoring agricultural drought in a semiarid region and (2) using the identified drought index method, develop and evaluate time series of that drought index for the Texas High Plains. Based on the literature review, the Standardized Precipitation-Evapotranspiration Index (SPEI) was found to satisfy identified constraints for assessing agricultural drought. However, the SPEI was revised by replacing reference ET with potential crop ET to better represent actual water demand. Data from the Texas High Plains Evapotranspiration network was used to calculate SPEIs for the major irrigated crops. Trends and magnitudes of crop-specific, time-series SPEIs followed crop water demand patterns for summer crops. Such an observation suggests that a modified SPEI is an appropriate index to monitor agricultural drought for summer crops, but it was found to not account for soil water stored during the summer fallow period for winter wheat. C1 [Moorhead, Jerry E.; Gowda, Prasanna H.; Howell, Terry A.] ARS, Conservat & Prod Res Lab, USDA, Bushland, TX 79012 USA. [Singh, Vijay P.] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Porter, Dana O.] Texas A&M AgriLife Res & Extens Serv, Dept Biol & Agr Engn, Lubbock, TX 79403 USA. [Marek, Thomas H.] Texas A&M AgriLife Res, Dept Biol & Agr Engn, Amarillo, TX 79106 USA. [Stewart, B. A.] West Texas A&M Univ, Dryland Agr Inst, Canyon, TX 79016 USA. [Stewart, B. A.] West Texas A&M Univ, Dept Agr, Canyon, TX 79016 USA. RP Moorhead, JE (reprint author), ARS, Conservat & Prod Res Lab, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM jed.moorhead@ars.usda.gov FU USDA-ARS Ogallala Aquifer Program; West Texas AM University FX We gratefully acknowledge support from the USDA-ARS Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 40 TC 2 Z9 2 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2015 VL 51 IS 3 BP 807 EP 820 DI 10.1111/jawr.12275 PG 14 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CL2YO UT WOS:000356814300016 ER PT J AU Bieger, K Rathjens, H Allen, PM Arnold, JG AF Bieger, Katrin Rathjens, Hendrik Allen, Peter M. Arnold, Jeffrey G. TI Development and Evaluation of Bankfull Hydraulic Geometry Relationships for the Physiographic Regions of the United States SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE streams; fluvial geomorphology; bankfull discharge; nationwide and regional regression equations; hydrologic modeling ID CHANNEL GEOMETRY; RECURRENCE INTERVALS; ALLUVIAL CHANNELS; PERIOD DISCHARGE; STREAMS; RIVERS; WIDTH; UNCERTAINTY; ECOREGIONS; MORPHOLOGY AB Bankfull hydraulic geometry relationships are used to estimate channel dimensions for streamflow simulation models, which require channel geometry data as input parameters. Often, one nationwide curve is used across the entire United States (U.S.) (e.g., in Soil and Water Assessment Tool), even though studies have shown that the use of regional curves can improve the reliability of predictions considerably. In this study, regional regression equations predicting bankfull width, depth, and cross-sectional area as a function of drainage area are developed for the Physiographic Divisions and Provinces of the U.S. and compared to a nationwide equation. Results show that the regional curves at division level are more reliable than the nationwide curve. Reliability of the curves depends largely on the number of observations per region and how well the sample represents the population. Regional regression equations at province level yield even better results than the division-level models, but because of small sample sizes, the development of meaningful regression models is not possible in some provinces. Results also show that drainage area is a less reliable predictor of bankfull channel dimensions than bankfull discharge. It is likely that the regional curves can be improved using multiple regression models to incorporate additional explanatory variables. C1 [Bieger, Katrin] Texas A&M AgriLife, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Rathjens, Hendrik] Purdue Univ, Earth Atmospher & Planetary Sci, W Lafayette, IN 47907 USA. [Allen, Peter M.] Baylor Univ, Dept Geol, Waco, TX 76798 USA. [Arnold, Jeffrey G.] ARS, Grassland Soil & Water Res Lab, USDA, Temple, TX 76502 USA. RP Bieger, K (reprint author), Texas A&M AgriLife, Blackland Res & Extens Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. EM kbieger@brc.tamus.edu NR 91 TC 4 Z9 4 U1 3 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2015 VL 51 IS 3 BP 842 EP 858 DI 10.1111/jawr.12282 PG 17 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CL2YO UT WOS:000356814300019 ER PT J AU Dassanayake, RP Truscott, TC Zhuang, DY Schneider, DA Madsen-Bouterse, SA Young, AJ Stanton, JB Davis, WC O'Rourke, KI AF Dassanayake, Rohana P. Truscott, Thomas C. Zhuang, Dongyue Schneider, David A. Madsen-Bouterse, Sally A. Young, Alan J. Stanton, James B. Davis, William C. O'Rourke, Katherine I. TI Classical natural ovine scrapie prions detected in practical volumes of blood by lamb and transgenic mouse bioassays SO JOURNAL OF VETERINARY SCIENCE LA English DT Article DE B lymphocytes; blood; prions; scrapie; Tg338 mice ID LYMPHOID-TISSUE; IMMUNOHISTOCHEMICAL DETECTION; PRECLINICAL DIAGNOSIS; INFECTED SHEEP; PRPSC; PROTEIN; TRANSMISSION; ACCUMULATION; INFECTIVITY; BIOPSY AB Scrapie is diagnosed antemortem in sheep by detecting misfolded isoforms of prion protein (PrPsc) in lymphoid follicles of the rectal mucosa and nictitating membranes. Assay sensitivity is limited if (a) the biopsy is collected early during disease development, (b) an insufficient number of follicles is collected, or (c) peripheral accumulation of PrPSc is reduced or delayed. A blood test would be convenient for mass live animal scrapie testing. Currently approved techniques, however, have their own detection limits. Novel detection methods may soon offer anon-animal-based, rapid platform with detection sensitivities that rival the prion bioassay. In anticipation, we sought to determine if diseased animals could be routinely identified with a bioassay using B lymphocytes isolated from blood sample volumes commonly collected for diagnostic purposes in small ruminants. Scrapie transmission was detected in five of six recipient lambs intravenously transfused with B lymphocytes isolated from 5 similar to 10 mL of blood from a naturally scrapie-infected sheep. Additionally, scrapie transmission was observed in 18 ovinized transgenic Tg338 mice intracerebrally inoculated with B lymphocytes isolated from 5 similar to 10 mL of blood from two naturally scrapie-infected sheep. Based on our findings, we anticipate that these blood sample volumes should be of diagnostic value. C1 [Dassanayake, Rohana P.; Madsen-Bouterse, Sally A.; Stanton, James B.; Davis, William C.; O'Rourke, Katherine I.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Truscott, Thomas C.; Zhuang, Dongyue; Schneider, David A.] USDA, Anim Dis Res Unit, ARS, Pullman, WA 99164 USA. [Young, Alan J.] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. RP Dassanayake, RP (reprint author), Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM rohana1@vetmed.wsu.edu RI Stanton, James/A-5277-2011 OI Stanton, James/0000-0002-7661-2631 FU United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (The Current Research Information System) [5348-32000-030-00 D] FX This work was supported by funds from the United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (The Current Research Information System 5348-32000-030-00 D). The authors would like to thank Ms. Linda Hamburg and Ms. Lori Fuller for their assistance with the blood transfusion experiments; Ms. Desiree Lesiak for PRNP genotyping (USDA, ARS, Animal Disease Research Unit); and Mr. Christopher Schachtschneider and Ms. Jan Luft (Washington State University) for care of the animals. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 34 TC 3 Z9 3 U1 1 U2 2 PU KOREAN SOC VETERINARY SCIENCE PI SEOUL PA SEOUL NATL UNIV, COLLEGE VETERINARY MEDICINE,, SEOUL, 151-742, SOUTH KOREA SN 1229-845X EI 1976-555X J9 J VET SCI JI J. Vet. Sci. PD JUN PY 2015 VL 16 IS 2 BP 179 EP 186 DI 10.4142/jvs.2015.16.2.179 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CL4EO UT WOS:000356905600007 PM 25549221 ER PT J AU Stappen, I Tabanca, N Ali, A Wedge, DE Wanner, J Kaul, VK Lal, B Jaitak, V Gochev, VK Schmidt, E Jirovetz, L AF Stappen, Iris Tabanca, Nurhayat Ali, Abbas Wedge, David E. Wanner, Juergen Kaul, Vijay K. Lal, Brij Jaitak, Vikas Gochev, Velizar K. Schmidt, Erich Jirovetz, Leopold TI Chemical Composition and Biological Activity of Essential Oils from Wild Growing Aromatic Plant Species of Skimmia laureola and Juniperus macropoda from Western Himalaya SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Skimmia laureola; Juniperus macropoda; Essential oil; Antifungal activity; Antibacterial activity; Biting deterrent activity; Larvicidal activity ID ANTIMICROBIAL ACTIVITY; ANTIFUNGAL ACTIVITY; MEDICINAL-PLANTS; COMPONENTS AB The Himalayan region is very rich in a great variety of medicinal plants. In this investigation the essential oils of two selected species are described for their antimicrobial and larvicidal as well as biting deterrent activities. Additionally, the odors are characterized. Analyzed by simultaneous GC-MS and GC-FID, the essential oils' chemical compositions are given. The main components of Skimmia laureola oil were linalool and linalyl acetate whereas sabinene was found as the main compound for Juniperus macropoda essential oil. Antibacterial testing by agar dilution assay revealed highest activity of S. laureola oil against all tested bacteria, followed by J. macropoda oil. Antifungal activity was evaluated against the strawberry anthracnose causing plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. Juniperus macropoda essential oil indicated higher antifungal activity against all three pathogens than S. laureola oil. Both essential oils showed biting deterrent activity above solvent control but low larvicidal activity. C1 [Stappen, Iris; Schmidt, Erich; Jirovetz, Leopold] Univ Vienna, Dept Med Chem, Vienna, Austria. [Tabanca, Nurhayat; Ali, Abbas] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Wedge, David E.] Univ Mississippi, USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Wanner, Juergen] Kurt Kitzing Co, Wallerstein, Germany. [Kaul, Vijay K.] Inst Himalayan Bioresource Technol, CSIR, Palampur 176061, Himachal Prades, India. [Lal, Brij] Inst Himalayan Bioresource Technol, CSIR, Biodivers Div, Palampur 176061, Himachal Prades, India. [Jaitak, Vikas] Cent Univ Punjab, Ctr Chem & Pharmaceut Sci, Bathinda, India. [Gochev, Velizar K.] Paisij Hilendarski Univ Plovdiv, Dept Biochem & Microbiol, BG-4000 Plovdiv, Bulgaria. RP Stappen, I (reprint author), Univ Vienna, Dept Med Chem, Vienna, Austria. EM iris.stappen@univie.ac.at FU USDA/ARS [56-6402-1-612]; Deployed War-Fighter Protection research program - U.S. Department of Defense through the Armed Forces Pest Management Board FX This study was supported in part by USDA/ARS grant No. 56-6402-1-612, Deployed War-Fighter Protection research program grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. We thank Ms. J.L. Robertson, Ms. R. Pace for performing bioassays Dr. James J. Becnel, Mosquito and Fly Research Unit USDA-ARS, Gainesville, for supplying Ae. aegypti eggs. Authors V.K Kaul, B. Lal and V. Jaitak are thankful to the Director CSIR-IHBT Palampur for providing necessary facilities for plant survey, collection of plant materials, hydrodistillations and analytical work. NR 24 TC 0 Z9 0 U1 3 U2 6 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD JUN PY 2015 VL 10 IS 6 BP 1071 EP 1074 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA CK9NB UT WOS:000356565700067 PM 26197554 ER PT J AU Rahman, A Wallis, CM Uddin, W AF Rahman, Alamgir Wallis, Christopher M. Uddin, Wakar TI Silicon-Induced Systemic Defense Responses in Perennial Ryegrass Against Infection by Magnaporthe oryzae SO PHYTOPATHOLOGY LA English DT Article ID GRAY LEAF-SPOT; MILDEW BLUMERIA-GRAMINIS; RICE BLAST FUNGUS; POWDERY MILDEW; INDUCED RESISTANCE; GENE-EXPRESSION; CELL-WALL; PODOSPHAERA-PANNOSA; GRISEA INTERACTIONS; WEEPING LOVEGRASS AB Sustainable integrated disease management for gray leaf spot of perennial ryegrass may involve use of plant defense elicitors with compatible traditional fungicides to reduce disease incidence and severity. Silicon (Si) has previously been identified as a potential inducer or modulator of plant defenses against different fungal pathogens. To this end, perennial ryegrass was inoculated with the causal agent of gray leaf spot, Magnaporthe oryzae, when grown in soil that was nonamended or amended with three different levels of calcium silicate (1, 5, or 10 metric tons [t]/ha). When applied at a rate of 5 t/ha, calcium silicate was found to significantly suppress gray leaf spot in perennial ryegrass, including a significant reduction of disease incidence (39.5%) and disease severity (47.3%). Additional studies observed non-penetrated papillae or cell-wall appositions harboring callose, phenolic autofluorogens, and lignin-associated polyphenolic compounds in grass grown in the Si-amended soil. Regarding defense-associated enzyme levels, only following infection did grass grown in Si-amended soil exhibit greater activities of peroxidase and polyphenol oxidase than equivalent inoculated control plants. Also following infection with M. oryzae, grass levels of several phenolic acids, including chlorogenic acid and flavonoids, and relative expression levels of genes encoding phenylalanine ammonia lyase (PALa and PALb) and lipoxygenase (LOXa) significantly increased in Si-amended plants compared with that of nonamended control plants. These results suggest that Si-mediated increase of host defense responses to fungal pathogens in perennial ryegrass has a great potential to be part of an effective integrated disease management strategy against gray leaf spot development. C1 [Rahman, Alamgir; Uddin, Wakar] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Wallis, Christopher M.] Agr Res Serv, USDA, San Joaquin Valley Agr Sci, Parlier, CA 93648 USA. RP Uddin, W (reprint author), Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. EM wxu2@psu.edu NR 56 TC 4 Z9 4 U1 7 U2 26 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2015 VL 105 IS 6 BP 748 EP 757 DI 10.1094/PHYTO-12-14-0378-R PG 10 WC Plant Sciences SC Plant Sciences GA CL4VY UT WOS:000356955500004 PM 25738553 ER PT J AU Funnell-Harris, DL Sattler, SE O'Neill, PM Eskridge, KM Pedersen, JF AF Funnell-Harris, Deanna L. Sattler, Scott E. O'Neill, Patrick M. Eskridge, Kent M. Pedersen, Jeffrey F. TI Effect of waxy (Low Amylose) on Fungal Infection of Sorghum Grain SO PHYTOPATHOLOGY LA English DT Article ID FUSARIUM-MONILIFORME; ANTIFUNGAL PROTEINS; MOLD RESISTANCE; MAIZE; ENDOSPERM; STARCH; PLANTS; DIGESTIBILITY; PATHOGENS; OPAQUE-2 AB Loss of function mutations in waxy, encoding granule bound starch synthase (GBSS) that synthesizes amylose, results in starch granules containing mostly amylopectin. Low amylose grain with altered starch properties has increased usability for feed, food, and grain-based ethanol. In sorghum, two classes of waxy (wx) alleles had been characterized for absence or presence of GBSS: wx(a) (GBSS(-)) and wx(b) (GBSS(+), with reduced activity). Field-grown grain of wild-type; waxy, GBSS(-); and waxy, GBSS(+) plant introduction accessions were screened for fungal infection. Overall, results showed that waxy grains were not more susceptible than wild-type. GBSS- and wild-type grain had similar infection levels. However, height was a factor with waxy, GBSS(+) lines: short accessions (wxb allele) were more susceptible than tall accessions (undescribed allele). In greenhouse experiments, grain from accessions and near-isogenic wx(a), wx(b), and wild-type lines were inoculated with Alternaria sp., Fusarium thapsinum, and Curvularia sorghina to analyze germination and seedling fitness. As a group, waxy lines were not more susceptible to these pathogens than wildtype, supporting field evaluations. After C. sorghina and F. thapsinum inoculations most waxy and wild-type lines had reduced emergence, survival, and seedling weights. These results are valuable for developing waxy hybrids with resistance to grain-infecting fungi. C1 [Funnell-Harris, Deanna L.; O'Neill, Patrick M.] Univ Nebraska, GFBRU, USDA ARS, Lincoln, NE 68583 USA. [Funnell-Harris, Deanna L.; O'Neill, Patrick M.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Sattler, Scott E.; Pedersen, Jeffrey F.] Univ Nebraska, GFBRU, USDA ARS, Dept Agron & Hort, Lincoln, NE 68583 USA. [Eskridge, Kent M.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. RP Funnell-Harris, DL (reprint author), Univ Nebraska, GFBRU, USDA ARS, Lincoln, NE 68583 USA. EM Deanna.Funnell-Harris@ars.usda.gov FU USDA-ARS, CRIS project [5440-21220-032-00D] FX We thank J. Toy for overseeing field operations and producing greenhouse-grown grain; J. Toy and N. Palmer for valuable discussions; and K. Wohlgemuth, K. Drudik, and M. Sederberg for laboratory assistance. Funding was provided by the USDA-ARS, CRIS project 5440-21220-032-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 72 TC 1 Z9 1 U1 3 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2015 VL 105 IS 6 BP 786 EP 796 DI 10.1094/PHYTO-09-14-0255-R PG 11 WC Plant Sciences SC Plant Sciences GA CL4VY UT WOS:000356955500009 PM 25626075 ER PT J AU Lin, H Islam, MS Cabrera-La Rosa, JC Civerolo, EL Groves, RL AF Lin, Hong Islam, Md Sajedul Cabrera-La Rosa, Juan C. Civerolo, Edwin L. Groves, Russell L. TI Population Structure of Xylella fastidiosa Associated with Almond Leaf Scorch Disease in the San Joaquin Valley of California SO PHYTOPATHOLOGY LA English DT Article DE annual variation ID SEQUENCE REPEAT MARKERS; UNITED-STATES; GENETIC DIVERSITY; PIERCES-DISEASE; CLONAL DIVERSITY; CAUSAL BACTERIUM; SUBSP MULTIPLEX; OLIVE TREES; STRAINS; OLEANDER AB Xylella fastidiosa causes disease in many commercial crops, including almond leaf scorch (ALS) disease in susceptible almond (Prunus dulcis). In this study, genetic diversity and population structure of X. fastidiosa associated with ALS disease were evaluated. Isolates obtained from two almond orchards in Fresno and Kern County in the San Joaquin Valley of California were analyzed for two successive years. Multilocus simple-sequence repeat (SSR) analysis revealed two major genetic clusters that were associated with two host cultivars, 'Sonora' and 'Nonpareil', respectively, regardless of the year of study or location of the orchard. These relationships suggest that host cultivar selection and adaptation are major driving forces shaping ALS X. fastidiosa population structure in the San Joaquin Valley. This finding will provide insight into understanding pathogen adaptation and host selection in the context of ALS disease dynamics. C1 [Lin, Hong; Islam, Md Sajedul; Civerolo, Edwin L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Islam, Md Sajedul] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Cabrera-La Rosa, Juan C.] Univ Privada Antenor Orrego, Trujillo, Peru. [Groves, Russell L.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. RP Lin, H (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM Hong.Lin@ars.usda.gov FU USDA-ARS Xylella fastidiosa CRIS Project FX We thank P. Sahota and M. Schreiber for technical assistance. Funding for this project was provided by the USDA-ARS Xylella fastidiosa CRIS Project. Trade names or commercial products in this publication are mentioned solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal employment opportunity provider. NR 62 TC 0 Z9 0 U1 4 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2015 VL 105 IS 6 BP 825 EP 832 DI 10.1094/PHYTO-09-14-0254-R PG 8 WC Plant Sciences SC Plant Sciences GA CL4VY UT WOS:000356955500013 PM 25807309 ER PT J AU Edwards, MC Weiland, JJ Todd, J Stewart, LR AF Edwards, Michael C. Weiland, John J. Todd, Jane Stewart, Lucy R. TI Infectious Maize rayado fino virus from Cloned cDNA SO PHYTOPATHOLOGY LA English DT Article ID BLUE-DWARF-VIRUS; YELLOW MOSAIC TYMOVIRUS; ETCHED-LINE VIRUS; VASCULAR PUNCTURE; MOLECULAR CHARACTERIZATION; MECHANICAL TRANSMISSION; EXPRESSION STRATEGY; GENUS MARAFIVIRUS; LEAFHOPPER VECTOR; DALBULUS-MAIDIS AB A full-length cDNA clone was produced from a U.S. isolate of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus within the family Tymoviridae. Infectivity of transcripts derived from cDNA clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors Dalbulus maidis and Graminella nigrifrons that transmit the virus in a persistent-propagative manner. Infection of maize plants through vascular puncture inoculation of seed with transcript RNA resulted in the induction of fine stipple stripe symptoms typical of those produced by wild-type MRFV and a frequency of infection comparable with that of the wild type. Northern and Western blotting confirmed the production of MRFV-specific RNAs and proteins in infected plants and protoplasts. An unanticipated increase in subgenomic RNA synthesis over levels in infected plants was observed in protoplasts infected with either wild-type or cloned virus. A conserved cleavage site motif previously demonstrated to function in both Oat blue dwarf virus capsid protein and tymoviral nonstructural protein processing was identified near the amino terminus of the MRFV replicase polyprotein, suggesting that cleavage at this site also may occur. C1 [Edwards, Michael C.; Weiland, John J.] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Todd, Jane; Stewart, Lucy R.] USDA ARS, Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. RP Edwards, MC (reprint author), USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. EM michael.edwards@ars.usda.gov FU USDA-ARS CRIS project [5442-22000-048-00D] FX Funding for this work was provided by USDA-ARS CRIS project number 5442-22000-048-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 42 TC 2 Z9 2 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2015 VL 105 IS 6 BP 833 EP 839 DI 10.1094/PHYTO-09-14-0250-R PG 7 WC Plant Sciences SC Plant Sciences GA CL4VY UT WOS:000356955500014 PM 25651051 ER PT J AU Bag, S Al Rwahnih, M Li, A Gonzalez, A Rowhani, A Uyemoto, JK Sudarshana, MR AF Bag, Sudeep Al Rwahnih, Maher Li, Ashley Gonzalez, Asaul Rowhani, Adib Uyemoto, Jerry K. Sudarshana, Mysore R. TI Detection of a New Luteovirus in Imported Nectarine Trees: A Case Study to Propose Adoption of Metagenomics in Post-Entry Quarantine SO PHYTOPATHOLOGY LA English DT Article ID GENE-EXPRESSION; GENOME SEQUENCE; DWARF DISEASE; UNITED-STATES; SMALL RNAS; DNA VIRUS; PLUM POX; GRAPEVINE; IDENTIFICATION; FLORIDA AB In spring 2013, 5-year-old nectarine (Prunus persica) trees, grafted on peach rootstock Nemaguard, were found stunted in a propagation block in California. These trees had been propagated from budwood of three nectarine cultivars imported from France and cleared through the post-entry quarantine procedure. Examination of the canopy failed to reveal any obvious symptoms. However, examination of the trunks, after stripping the bark, revealed extensive pitting on the woody cylinder. To investigate the etiological agent, double-stranded RNA was extracted from bark scrapings from the scion and rootstock portions, and a cDNA library was prepared and sequenced using the Illumina platform. BLAST analysis of the contigs generated by the de novo assembly of sequence reads indicated the presence of a novel luteovirus. Complete sequence of the viral genome was determined by sequencing of three overlapping cDNA clones generated by reverse transcription-polymerase chain reaction (RT-PCR) and by rapid amplification of the 59- and 39-termini. The virus genome was comprised of 4,991 nucleotides with a gene organization similar to members of the genus Luteovirus (family Luteoviridae). The presence of the virus, tentatively named Nectarine stem pitting-associated virus, was confirmed in symptomatic trees by RT-PCR. Discovery of a new virus in nectarine trees after post-entry quarantine indicates the importance of including (i) metagenomic analysis by next-generation sequencing approach as an essential tool to assess the plant health status, and (ii) examination of the woody cylinders as part of the indexing process. C1 [Bag, Sudeep; Al Rwahnih, Maher; Rowhani, Adib] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Li, Ashley; Gonzalez, Asaul; Uyemoto, Jerry K.; Sudarshana, Mysore R.] Univ Calif Davis, USDA, Agr Res Serv, Dept Plant Pathol, Davis, CA 95616 USA. RP Sudarshana, MR (reprint author), Univ Calif Davis, USDA, Agr Res Serv, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA. EM mysore.sudarshana@ars.usda.gov FU USDA-ARS CRIS [5306-22000-014-00D] FX This project was supported by USDA-ARS CRIS 5306-22000-014-00D. The authors acknowledge the cooperation of a grower for providing access to trees used in the study. Helpful comments by anonymous reviewers of this manuscript are much appreciated. Mention of a trademark, proprietary products, or vendor does not constitute guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 41 TC 6 Z9 6 U1 5 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2015 VL 105 IS 6 BP 840 EP 846 DI 10.1094/PHYTO-09-14-0262-R PG 7 WC Plant Sciences SC Plant Sciences GA CL4VY UT WOS:000356955500015 PM 25775105 ER PT J AU Lehotay, SJ Sapozhnikova, Y Mol, HGJ AF Lehotay, Steven J. Sapozhnikova, Yelena Mol, Hans G. J. TI Current issues involving screening and identification of chemical contaminants in foods by mass spectrometry SO TRAC-TRENDS IN ANALYTICAL CHEMISTRY LA English DT Article DE Chemical contaminants; Confirmation; Cytotoxin; Mycotoxins; Identification; Mass spectrometry; Pesticides; Screening; Residues; Veterinary drugs ID PERFORMANCE LIQUID-CHROMATOGRAPHY; PESTICIDE-RESIDUE ANALYSIS; ION MOBILITY SPECTROMETRY; INFORMATION-DEPENDENT ACQUISITION; PRESSURE DESORPTION IONIZATION; SUPERSONIC MOLECULAR-BEAMS; VETERINARY DRUG RESIDUES; FLOW-INJECTION ANALYSIS; GAS-CHROMATOGRAPHY; QUADRUPOLE-TIME AB Although quantitative analytical methods must be empirically validated prior to their use in a variety of applications, including regulatory monitoring of chemical adulterants in foods, validation of qualitative method performance for the analytes and matrices of interest is frequently ignored, or general guidelines are assumed to be true for specific situations. Just as in the case of quantitative method validation, acceptable method performance criteria should be established for qualitative analysis purposes to suit the analytical needs for given applications, and empirical method validation should be conducted to demonstrate the qualitative performance capabilities the method. This critical review article is intended to describe and discuss recent developments with of respect to qualitative aspects in mass spectrometry, and to make recommendations for validation of qualitative methods that meet common needs for monitoring of chemical contaminants in foods. Published by Elsevier B.V. C1 [Lehotay, Steven J.; Sapozhnikova, Yelena] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Mol, Hans G. J.] Wageningen UR, RIKILT, NL-6708 WB Wageningen, Netherlands. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM steven.lehotay@ars.usda.gov NR 104 TC 19 Z9 19 U1 9 U2 58 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0165-9936 EI 1879-3142 J9 TRAC-TREND ANAL CHEM JI Trac-Trends Anal. Chem. PD JUN PY 2015 VL 69 SI SI BP 62 EP 75 DI 10.1016/j.trac.2015.02.012 PG 14 WC Chemistry, Analytical SC Chemistry GA CL1XC UT WOS:000356737200014 ER PT J AU McCoin, CS Knotts, TA Ono-Moore, KD Oort, PJ Adams, SH AF McCoin, Colin S. Knotts, Trina A. Ono-Moore, Kikumi D. Oort, Pieter J. Adams, Sean H. TI Long-chain acylcarnitines activate cell stress and myokine release in C2C12 myotubes: calcium-dependent and -independent effects SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE cell death; interleukin-6; skeletal muscle ID FATTY-ACID OXIDATION; PALMITOYLTRANSFERASE-II DEFICIENCY; MUSCLE INSULIN-RESISTANCE; COENZYME-A; SARCOPLASMIC-RETICULUM; VENTRICULAR MYOCYTES; MASS-SPECTROMETRY; IN-VIVO; CARNITINE; MEMBRANE AB Acylcarnitines, important lipid biomarkers reflective of acyl-CoA status, are metabolites that possess bioactive and inflammatory properties. This study examined the potential for long-chain acylcarnitines to activate cellular inflammatory, stress, and death pathways in a skeletal muscle model. Differentiated C2C12 myotubes treated with L-C14, C16, C18, and C18:1 carnitine displayed dose-dependent increases in IL-6 production with a concomitant rise in markers of cell permeability and death, which was not observed for shorter chain lengths. L-C16 carnitine, used as a representative long-chain acylcarnitine at initial extracellular concentrations >= 25 mu M, increased IL-6 production 4.1-, 14.9-, and 31.4-fold over vehicle at 25, 50, and 100 mu M. Additionally, L-C16 carnitine activated c-Jun NH2-terminal kinase, extracellular signal-regulated kinase, and p38 mitogen-activated protein kinase between 2.5- and 11-fold and induced cell injury and death within 6 h with modest activation of the apoptotic caspase-3 protein. L-C16 carnitine rapidly increased intracellular calcium, most clearly by 10 mu M, implicating calcium as a potential mechanism for some activities of long-chain acylcarnitines. The intracellular calcium chelator BAPTA-AM blunted L-C16 carnitine-mediated IL-6 production by >65%. However, BAPTA-AM did not attenuate cell permeability and death responses, indicating that these outcomes are calcium independent. The 16-carbon zwitterionic compound amidosulfobetaine-16 qualitatively mimicked the L-C16 carnitine-associated cell stress outcomes, suggesting that the effects of high experimental concentrations of long-chain acylcarnitines are through membrane disruption. Herein, a model is proposed in which acylcarnitine cell membrane interactions take place along a spectrum of cellular concentrations encountered in physiological-to-pathophysiological conditions, thus regulating function of membrane-based systems and impacting cell biology. C1 [McCoin, Colin S.; Adams, Sean H.] Univ Calif Davis, Mol Cellular & Integrat Physiol Grad Grp, Davis, CA 95616 USA. [Knotts, Trina A.; Ono-Moore, Kikumi D.; Oort, Pieter J.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA USA. [Knotts, Trina A.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Adams, Sean H.] Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA. [Adams, Sean H.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. RP Adams, SH (reprint author), Arkansas Childrens Nutr Ctr, 15 Childrens Way, Little Rock, AR 72202 USA. EM SHAdams@uams.edu OI McCoin, Colin/0000-0002-8557-6441 FU American Diabetes Association [1-12-BS-02]; National Institutes of Health (NIH) [R01-DK-078328, R01-DK-078328-02S1]; United States Department of Agriculture-Agricultural Research Service intramural Project [5306-51530-019-00]; T32 predoctoral training award; National Center for Advancing Translational Sciences, NIH [UL1-TR-000002, TL1-TR-000133] FX This work was supported by the American Diabetes Association (1-12-BS-02), the National Institutes of Health (NIH) (R01-DK-078328 and R01-DK-078328-02S1), and United States Department of Agriculture-Agricultural Research Service intramural Project 5306-51530-019-00. This project was also supported by a T32 predoctoral training award (to C. S. McCoin), funded by the National Center for Advancing Translational Sciences, NIH, through Grant no. UL1-TR-000002 and linked award TL1-TR-000133. NR 43 TC 5 Z9 5 U1 4 U2 8 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUN 1 PY 2015 VL 308 IS 11 BP E990 EP E1000 DI 10.1152/ajpendo.00602.2014 PG 11 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA CK5ID UT WOS:000356256100007 PM 25852008 ER PT J AU Meier, A Jansky, S Halterman, D AF Meier, Austin Jansky, Shelley Halterman, Dennis TI Germplasm Release: Three Potato Clones Incorporating Combined Resistances to Early Blight from S. palustre and Late Blight from S. bulbocastanum into a S. tuberosum Background SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato; Solanum tuberosum; Solanum palustre; Solanum bulbocastanum; Early blight; Late blight; Disease resistance ID SOLANUM-BULBOCASTANUM; SOMATIC HYBRIDS; BREVIDENS; CULTIVAR AB Three clones in a segregating population derived from a cross between the disease resistant parents +297 and K41 are being released as germplasm with resistance to both early blight, caused by Alternaria solani, and late blight, caused by Phytophthora infestans. The source of resistance to early blight in +297 is the wild species S. palustre and late blight resistance in K41 is conferred by the RB gene from S. bulbocastanum. These clones, named BR3, BR5, and BR85 (BR for Blight Resistant), yield well at a temperate zone latitude. In addition to containing heritable resistance to both early and late blights, these clones possess multiple other desirable agronomic traits, are fertile, and readily cross to cultivars. C1 [Jansky, Shelley; Halterman, Dennis] ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. [Meier, Austin; Jansky, Shelley] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. RP Halterman, D (reprint author), ARS, Vegetable Crops Res Unit, USDA, 1575 Linden Dr, Madison, WI 53706 USA. EM dennis.halterman@ars.usda.gov FU Wisconsin Potato and Vegetable Growers Association; Monsanto fellowship FX We would like to thank Dr. John Helgeson for providing resistant parents for this cross. Also thanks to Dr. Brian Perkins for determining the glycoalkaloid content of released clones. Funding was provided by the Wisconsin Potato and Vegetable Growers Association. Salary for A.M. was provided by a Monsanto fellowship. NR 12 TC 1 Z9 1 U1 1 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2015 VL 92 IS 3 BP 410 EP 416 DI 10.1007/s12230-015-9451-y PG 7 WC Agronomy SC Agriculture GA CK6KT UT WOS:000356337700012 ER PT J AU Bamberg, J Moehninsi Navarre, R Suriano, J AF Bamberg, John Moehninsi Navarre, Roy Suriano, Jana TI Variation for Tuber Greening in the Diploid Wild Potato Solanum Microdontum SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Greening; Wild potato; Glycoalkaloids ID FRESH-MARKET POTATOES; LIGHT EXPOSURE; GLYCOALKALOID CONTENT; CHLOROPHYLL; STORAGE; TEMPERATURE; CULTIVARS; ACCUMULATION; INHERITANCE; PLANT AB Greening of the tuber skin is an undesirable defect in fresh and processed potato. Tubers of 92 Solanum microdontum families represented by 12 individuals each were generated in the winter greenhouse in 2009-2010. These were evaluated in two trials of family bulks after 4 days of exposure to 200 ft candles of fluorescent white light at room temperature, similar to that observed in local grocery stores, and according to preliminary tests, able to turn some microdontum tubers very green. A qualitative score of green (G) or white (W) was assigned to each tuber. Tubers of about 10 % of families were scored as mostly W. In comparison, when 185 named Solanum tuberosum cultivars were illuminated in the same way, none from Europe or North America scored W. Two replicates of G and W individual tubers were selected from a variety of microdontum families and planted to create a new clonal generation of tubers. Those second generation daughter tubers showed a response to illumination which was consistent with that of the previous clonal generation. Finally, W and G clones were selected and mated. All GxG hybrids were uniformly G, but only three WxW crosses produced families that were nearly uniformly W. Thus some selections were shown to have consistent non-greening over trials spaced in time, over a clonal generation, and, when crossed together produced pure non-greening progeny. On the other hand, several very green selections similarly were consistently very green over time, clonal regeneration, and intermating. Tests of tuber tissue for glycoalkaloids showed that some illuminated clones neither green nor accumulate glycoalkaloids. These materials would make good models for further study of inheritance and physiology of the greening trait, and for breeding. C1 [Bamberg, John; Suriano, Jana] ARS, USDA, US Potato Genebank, Sturgeon Bay, WI 54235 USA. [Navarre, Roy] ARS, USDA, Prosser, WA USA. [Moehninsi] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. RP Bamberg, J (reprint author), ARS, USDA, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. EM John.Bamberg@ars.usda.gov FU Peninsular Agricultural Research Station FX The authors thank Mrs. Adele Douglass for technical assistance and first noticing the great greening differential among microdontum populations, and the Peninsular Agricultural Research Station program and staff for their support. NR 42 TC 1 Z9 1 U1 5 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2015 VL 92 IS 3 BP 435 EP 443 DI 10.1007/s12230-015-9454-8 PG 9 WC Agronomy SC Agriculture GA CK6KT UT WOS:000356337700015 ER PT J AU Heidari, M Fitzgerald, SD Zhang, HM AF Heidari, Mohammad Fitzgerald, Scott D. Zhang, Huanmin TI Immune Responses in Cecal Tonsils of Marek's Disease Virus-Infected Chickens SO AVIAN DISEASES LA English DT Article DE Marek's disease; cecal tonsil; toll-like receptors; real-time PCR ID TOLL-LIKE RECEPTORS; LYMPHOID-TISSUES; SIGNALING PATHWAYS; BURSA; CELLS; RECOGNITION; FABRICIUS; INNATE; THYMUS; GENES AB Marek's disease (MD) is a lymphoproliferative disease of domestic chickens that is caused by a highly cell-associated oncogenic a-herpesvirus, Marek's disease virus (MDV). MDV replicates in chicken lymphocytes and establishes a latent infection within CD4(+) T cells. Clinical signs of MD include depression, crippling, weight loss, bursal/thymic atrophy, neurologic disorders, and rapid onset of T cell lymphomas that infiltrate lymphoid tissues, visceral organs, and peripheral nerves. The cecal tonsils (CTs) are considered the largest lymphoid aggregates of avian gut-associated lymphoid tissue. Along with Peyer's patches, CTs elicit protective immune responses against bacterial and viral pathogens in the intestinal tract of avian species. In this study, we investigated the effect of MDV infection on toll-like receptor (TLR) gene expression in CTs of MD-susceptible (7(2)) and resistant (6(3)) chicken lines. Real-time PCR gene expression profiling revealed that of the 10 TLRs tested, TLR2A, TLR3, TLR5, and TLR15 displayed significant differential expression patterns at different time points postinoculation. The expression levels of the remaining six genes were minimally affected by MDV infection in either line. Immunohistochemical analysis showed a severe depletion of B cells and CD4(+) T cells in the CTs of susceptible line at 5 days postinfection (dpi), which recovered by 21 dpi. The destruction of B and T cells in the CTs of the resistant line was minimal at 5 dpi, which also recovered by 21 dpi. A significant infiltration of macrophages was observed after the depletion of B and T cells in the infected birds of both lines that could account for the differential TLR gene expression in the infected birds. The data presented provide further insight into the mechanism of MDV pathogenesis and tissue-specific immunologic responses to viral infection. C1 [Heidari, Mohammad; Zhang, Huanmin] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Fitzgerald, Scott D.] Michigan State Univ, Coll Vet Med, Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA. RP Heidari, M (reprint author), USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. EM mohammad.heidari@ars.usda.gov NR 48 TC 2 Z9 2 U1 1 U2 8 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2015 VL 59 IS 2 BP 213 EP 226 PG 14 WC Veterinary Sciences SC Veterinary Sciences GA CK9DH UT WOS:000356540300004 PM 26473671 ER PT J AU Gimeno, IM Cortes, AL Faiz, NM Hernandez-Ortiz, BA Guy, JS Hunt, HD Silva, RF AF Gimeno, Isabel M. Cortes, Aneg L. Faiz, Nik M. Hernandez-Ortiz, Byron A. Guy, James S. Hunt, Henry D. Silva, Robert F. TI Evaluation of the Protection Efficacy of a Serotype 1 Marek's Disease Virus-Vectored Bivalent Vaccine Against Infectious Laryngotracheitis and Marek's Disease SO AVIAN DISEASES LA English DT Article DE Marek's disease; laryngotracheitis; poultry; avian; vaccine; control; herpesvirus ID RECOMBINANT FOWLPOX VIRUS; STRAIN CVI 988; GLYCOPROTEIN-B; NEWCASTLE-DISEASE; FIELD TRIALS; IN-OVO; CHICKENS; HERPESVIRUS; GENES; REPLICATION AB Laryngotracheitis (LT) is a highly contagious respiratory disease of chickens that produces significant economic losses to the poultry industry. Traditionally, LT has been controlled by administration of modified live vaccines. In recent years, the use of recombinant DNA-derived vaccines using turkey herpesvirus (HVT) and fowlpox virus has expanded, as they protect not only against the vector used but also against LT. However, HVT-based vaccines confer limited protection against challenge, with emergent very virulent plus Marek's disease virus (vv+MDV). Serotype 1 vaccines have been proven to be the most efficient against vv+MDV. In particular, deletion of oncogene MEQ from the oncogenic vvMDV strain Md5 (BAC Delta MEQ) resulted in a very efficient vaccine against vv+MDV. In this work, we have developed two recombinant vaccines against MD and LT by using BAC Delta MEQ as a vector that carries either the LT virus (LTV) gene glycoprotein B (gB; BAC Delta MEQ-gB) or LTV gene glycoprotein J (gJ; BAC Delta MEQ-gJ). We have evaluated the protection that these recombinant vaccines confer against MD and LT challenge when administered alone or in combination. Our results demonstrated that both bivalent vaccines (BAC Delta MEQ-gB and BAC Delta MEQ-gJ) replicated in chickens and were safe to use in commercial meat-type chickens bearing maternal antibodies against MDV. BAC Delta MEQ-gB protected as well as a commercial recombinant (r)HVT-LT vaccine against challenge with LTV. However, BAC Delta MEQ-gJ did not protect adequately against LT challenge or increase protection conferred by BAC Delta MEQ-gB when administered in combination. On the other hand, both BAC Delta MEQ-gB and BAC Delta MEQ-gJ, administered alone or in combination, protected better against an early challenge with vv+MDV strain 648A than commercial strains of rHVT-LT or CVI988. Our results open a new avenue in the development of recombinant vaccines by using serotype 1 MDV as vectors. C1 [Gimeno, Isabel M.; Cortes, Aneg L.; Faiz, Nik M.; Hernandez-Ortiz, Byron A.; Guy, James S.] N Carolina State Univ, Sch Vet, Dept Populat Hlth & Pathobiol, Raleigh, NC 27607 USA. [Hunt, Henry D.; Silva, Robert F.] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Gimeno, IM (reprint author), N Carolina State Univ, Sch Vet, Dept Populat Hlth & Pathobiol, 1060 William Moore Dr, Raleigh, NC 27607 USA. EM imgimeno@ncsu.edu FU U.S. Poultry and Egg Association FX We thank Lonnie Milam for excellent technical assistance, personnel at the Laboratory Animal Resources for their help in the husbandry of the chickens, and to the U.S. Poultry and Egg Association for funding this project. NR 52 TC 1 Z9 2 U1 1 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2015 VL 59 IS 2 BP 255 EP 262 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CK9DH UT WOS:000356540300009 PM 26473676 ER PT J AU Bowman, AS Nolting, JM Massengill, R Baker, J Workman, JD Slemons, RD AF Bowman, Andrew S. Nolting, Jacqueline M. Massengill, Rose Baker, Joseph Workman, Jeffrey D. Slemons, Richard D. TI Influenza A Virus Surveillance in Waterfowl in Missouri, USA, 2005-2013 SO AVIAN DISEASES LA English DT Article DE influenza A virus; wild-waterfowl; Missouri; surveillance ID AVIAN INFLUENZA; SENTINEL DUCKS; UNITED-STATES; PARAMYXOVIRUSES; EPIZOOTIOLOGY; MINNESOTA; PATTERNS; MALLARDS; SUBTYPES; TURKEYS AB Missouri, United States, is located within the Mississippi Migratory Bird Flyway where wild waterfowl stop to feed and rest during migration and, weather permitting, to overwinter. Historically, Missouri has experienced sporadic influenza A virus (IAV) outbreaks in poultry and commercial swine. The introduction of IAVs from wild, migratory waterfowl is one possible source for the IAV, IAV genomic segments, or both involved in these outbreaks in key agricultural species. During 2005 through 2013, 3984 cloacal swabs were collected from hunter-harvested waterfowl in Missouri as part of an active IAV surveillance effort. Twenty-four avian species were represented in the sample population and 108 (2.7%) of the samples tested positive for IAV recovery. These IAV isolates represented 12 HA and nine NA subtypes and at least 27 distinct HA-NA combinations. An H14 IAV isolate recovered in Missouri during the sample period provided evidence for further establishment of the H14 subtype in North American wild waterfowl and gave proof that the previously rare subtype is more genetically diverse than previously detected. The present surveillance effort also produced IAV isolates that were genomically linked to the highly pathogenic H7N3 IAV strain that emerged in 2012 and caused severe disease in Mexico's domestic poultry. The presence of antigenically diverse IAV's circulating in wild waterfowl in the vicinity of commercial poultry and swine, along with the association of several wild-bird-lineage IAV genomic segments in viruses infecting poultry in North America, justifies continued attention to biosecurity efforts in food animal production systems and ongoing active IAV surveillance in wild birds. C1 [Bowman, Andrew S.; Nolting, Jacqueline M.; Workman, Jeffrey D.; Slemons, Richard D.] Ohio State Univ, Columbus, OH 43210 USA. [Massengill, Rose] USDA, Anim & Plant Hlth Inspect Serv, Jefferson City, MO 65109 USA. [Massengill, Rose; Baker, Joseph] Missouri Dept Agr, Jefferson City, MO 65102 USA. RP Bowman, AS (reprint author), Ohio State Univ, Dept Vet Prevent Med, 1920 Coffey Rd, Columbus, OH 43210 USA. EM bowman.214@osu.edu RI Bowman, Andrew/B-4321-2012 OI Bowman, Andrew/0000-0002-0738-8453 FU Ohio Department of Health; USDA Cooperative State Research, Education, and Extension Service (CSREES) Coordinated Agriculture Project (CAP), Avian Influenza Prevention and Control [20085520418863]; Centers of Excellence for Influenza Research and Surveillance (CEIRS), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN266200700007C, HHSN272201400006C] FX This work has been funded in part by the Ohio Department of Health; USDA Cooperative State Research, Education, and Extension Service (CSREES) Coordinated Agriculture Project (CAP), Avian Influenza Prevention and Control under award no. 20085520418863 and with federal funds from the Centers of Excellence for Influenza Research and Surveillance (CEIRS), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract Nos. HHSN266200700007C and HHSN272201400006C. NR 26 TC 0 Z9 0 U1 1 U2 8 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2015 VL 59 IS 2 BP 303 EP 308 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CK9DH UT WOS:000356540300015 PM 26473682 ER PT J AU Huff, GR Huff, WE Rath, NC Anthony, NB Nestor, KE AF Huff, G. R. Huff, W. E. Rath, N. C. Anthony, N. B. Nestor, K. E. TI Ascorbic Acid Differentially Affects Stress Response and Resistance to Colibacillosis in Turkeys from Genetic Lines Differing in Growth Rate SO AVIAN DISEASES LA English DT Article DE ascorbic acid; turkeys; transport stress; genetics; Escherichia coli ID INCREASED BODY-WEIGHT; COLI RESPIRATORY-INFECTION; ESCHERICHIA-COLI; BROILER-CHICKENS; VITAMIN-C; HEAT-STRESS; LYMPHOCYTE RATIO; OSTEOMYELITIS COMPLEX; POULTRY; IMMUNOSUPPRESSION AB Genetic selection for fast growth can affect the ability of male turkeys to cope with stressors, resulting in decreased immunity to opportunistic bacterial infection. The purpose of the current study was to compare the effects of ascorbic acid (AA) on the stress response and resistance to Escherichia coli challenge of birds selected for increased 16-wk body weight (BW; F-line) with their random-bred parent line (RBC2). Male turkeys were raised in duplicate floor pens in a two line 3 two AA treatment 3 two stress challenge (SC) design. At 5 wk of age, AA (1200 ppm) was provided in drinking water for a 24-hr period, during which all birds were weighed. After AA treatment, the SC group was subjected to a transport stress protocol. Six hours after the start of transport, SC birds were also inoculated in the thoracic air sac with 1 x 10(4) colony-forming units of E. coli. The following morning four birds from each pen were bled, and all birds were weighed and necropsied 2 days later. BW and gain after SC were decreased in the F-line but not the RBC2 line, and there were no AA effects on BW. The weight of the bursa of Fabricius relative to BW was higher in the RBC2 line than in the F-line, was decreased by SC, and was not affected by AA. The heterophil: lymphocyte ratio was higher in the SC F-line as compared to the SC RBC2 and was decreased by AA only in the SC F-line. Corticosterone (C) levels were increased by SC only in the F-line, and AA decreased C levels only in the RBC2 line. Airsacculitis scores were increased in the F-line SC birds. The challenge strain of E. coli was only detected in the air sac and liver of the AA-treated F-line SC birds and in the liver of the no-AA F-line birds. These results suggest that SC at 5 wk of age had a more deleterious effect on the fast-growing F-line than on its parent line and that AA may have increased susceptibility to colibacillosis in the SC F-line birds. C1 [Huff, G. R.; Huff, W. E.; Rath, N. C.] USDA ARS, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. [Anthony, N. B.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Nestor, K. E.] Ohio State Univ, Dept Anim Sci, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. RP Huff, GR (reprint author), USDA ARS, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. EM grhuff@uark.edu NR 44 TC 0 Z9 0 U1 2 U2 8 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2015 VL 59 IS 2 BP 323 EP 328 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CK9DH UT WOS:000356540300018 PM 26473685 ER PT J AU Carnaccini, S Crossley, B Breitmeyer, R Charlton, BR Bland, M Fowler, K De La Torre, F Torchetti, MK Wong, SS Wilson, D Jones, A Senties-Cue, CG AF Carnaccini, Silvia Crossley, Beate Breitmeyer, Richard Charlton, Bruce R. Bland, Mark Fowler, Kent De La Torre, Felicia Torchetti, Mia Kim Wong, Sook-San Wilson, Dennis Jones, Annette Senties-Cue, C. Gabriel TI Diagnosis and Control of a LPAI H5N8 Outbreak in a Japanese Quail (Coturnix coturnix japonica) Commercial Flock in the Central Valley of California SO AVIAN DISEASES LA English DT Article DE avian influenza; quail; California; LPAI; H5; duck; control; outbreak ID AVIAN INFLUENZA-VIRUS; UNITED-STATES; A VIRUSES; BIRDS; PATHOGENICITY; PATHOBIOLOGY; TRANSMISSION; PERSISTENCE; ADAPTATION; INFECTION AB In April 2014 an outbreak of low pathogenic avian influenza H5N8 North American genetic lineage was diagnosed in a commercial quail operation in Stanislaus County, California. Sudden increase in mortality prompted the submission of 20 Japanese quail hens (Coturnix c. japonica) to the California Animal Health and Food Safety Laboratory, Turlock Branch. Oropharyngeal and cloacal swabs tested positive for influenza A virus H5N8 by real-time reverse transcription-polymerase chain reaction. The virus was subsequently isolated. In vivo assay and sequencing of the hemagglutinin protein cleavage site classified the virus as a North American genetic lineage of low pathogenicity for chickens. Following the diagnosis, a rapid and coordinated response took place to contain the outbreak. The affected premise was depopulated, cleaned, and disinfected. Three areas from the affected premises-a 3 kilometer (km) radius (High Risk Zone), a 3-10 km area (Buffer Zone), and a 10-20 km (Surveillance Zone)-were established for avian influenza testing of commercial and noncommercial poultry operations. Surveillance testing and rapid control measures were successful in the control and eradication of the outbreak and revealed no area of spread of the virus from the index flock. This report describes the history, diagnosis, surveillance, and control measures applied to manage this outbreak. C1 [Carnaccini, Silvia; Charlton, Bruce R.; Senties-Cue, C. Gabriel] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, Turlock Branch, Sch Vet Med, Turlock, CA 95380 USA. [Crossley, Beate; Breitmeyer, Richard] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, Davis, CA 95616 USA. [Bland, Mark] Cutler Associates Int, Napa, CA 94558 USA. [Fowler, Kent; De La Torre, Felicia; Wilson, Dennis; Jones, Annette] Calif Dept Food & Agr, Anim Hlth & Food Safety Serv Div, Sacramento, CA 95814 USA. [Torchetti, Mia Kim] Natl Vet Serv Lab, Ames, IA 50010 USA. [Wong, Sook-San] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA. RP Senties-Cue, CG (reprint author), Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, Turlock Branch, Sch Vet Med, 1550 N Soderquist Rd, Turlock, CA 95380 USA. EM gsenties@cahfs.ucdavis.edu NR 22 TC 0 Z9 0 U1 2 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2015 VL 59 IS 2 BP 344 EP 348 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CK9DH UT WOS:000356540300022 PM 26473689 ER PT J AU Parys, KA Tewari, S Johnson, SJ AF Parys, Katherine A. Tewari, Sunil Johnson, Seth J. TI ADULTS OF THE WATERFERN WEEVIL, STENOPELMUS RUFINASUS GYLLENHAL (COLEOPTERA: CURCULIONIDAE), FEED ON A NON-HOST PLANT, SALVINIA MINIMA BAKER, IN LOUISIANA SO COLEOPTERISTS BULLETIN LA English DT Editorial Material ID LIFE-HISTORY C1 [Parys, Katherine A.; Tewari, Sunil; Johnson, Seth J.] Louisiana State Univ, Ctr Agr, Dept Entomol, LSU AgCtr, Baton Rouge, LA 70803 USA. [Parys, Katherine A.] USDA ARS, Southern Insect Res Management Unit, Stoneville, MS 38776 USA. [Tewari, Sunil] Dow AgroSci Midwest Res Ctr, Fowler, IN 47944 USA. RP Parys, KA (reprint author), Louisiana State Univ, Ctr Agr, Dept Entomol, LSU AgCtr, 404 Life Sci Bldg, Baton Rouge, LA 70803 USA. OI Parys, Katherine/0000-0001-7459-3687 NR 13 TC 0 Z9 0 U1 0 U2 3 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD JUN PY 2015 VL 69 IS 2 BP 316 EP 318 PG 3 WC Entomology SC Entomology GA CL0CA UT WOS:000356604900020 ER PT J AU Lee, CF Konstantinov, A AF Lee, Chi-Feng Konstantinov, Alexander TI A NEW SPECIES OF BURUMOSERIA CSIKI, 1939 FROM TAIWAN, WITH REDESCRIPTION OF THE GENUS AND NEW SYNONYMY OF LICYLLUS JACOBY, 1885 WITH THRASYCHROMA JACOBY, 1885 (COLEOPTERA: CHRYSOMELIDAE: GALERUCINAE: ALTICINI) SO COLEOPTERISTS BULLETIN LA English DT Article DE flea beetles; taxonomy; Oriental; Moseria; Erycibe henryi AB The Oriental genus Burumoseria Csiki, 1939 is redescribed. Burumoseria yuae Lee and Konstantinov, new species from Taiwan is described and illustrated. A key to Burumoseria species is provided. The Oriental and Australian genus Licyllus Jacoby, 1885 is synonymized with Thrasychroma Jacoby, 1885. C1 [Lee, Chi-Feng] Agr Res Inst Taiwan, Appl Zool Div, Taichung 413, Wufeng, Taiwan. [Konstantinov, Alexander] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist, Washington, DC 20560 USA. RP Lee, CF (reprint author), Agr Res Inst Taiwan, Appl Zool Div, 189 Chung Cheng Rd, Taichung 413, Wufeng, Taiwan. EM chifeng@tari.gov.tw FU Ministry of Science and Technology [MOST 103-2313-B-055-001] FX We thank the Taiwan Chrysomelid Research Team for assistance in collecting material, including Yi-Ting Chung, Bo-Xin Guo, Wen-Chuan Liao, Mei-Hua Tsou, Yu-Tung Wang, and Su-Fang Yu. We acknowledge the help of Jan Bezdek (Brno) in providing some literature. We especially thank Ta-Hsiang Lee for photographs of specimens, Mei-Hua Tsou for photographs of the host plant, and Chih-Kai Yang for identification of the host plants. This study was supported by the Ministry of Science and Technology (MOST 103-2313-B-055-001). Eleonore Dixon-Roche drew the habitus drawing of Burumoseria partita, Maayan Harel of Thrasychroma sp., and Mary Metz of Licyllus splendidus. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 6 TC 0 Z9 0 U1 1 U2 4 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD JUN PY 2015 VL 69 IS 2 BP 325 EP 331 PG 7 WC Entomology SC Entomology GA CL0CA UT WOS:000356604900023 ER PT J AU Nguyen, U Glenn, EP Nagler, PL Scott, RL AF Uyen Nguyen Glenn, Edward P. Nagler, Pamela L. Scott, Russell L. TI Long-term decrease in satellite vegetation indices in response to environmental variables in an iconic desert riparian ecosystem: the Upper San Pedro, Arizona, United States SO ECOHYDROLOGY LA English DT Article DE San Pedro River; Walnut Gulch Experimental Watershed; climate change; evapotranspiration; woody shrub encroachment; NDVI; enhanced vegetation index ID CLIMATE-CHANGE; FLUX TOWERS; EVAPOTRANSPIRATION; RIVER; TRANSPIRATION; PRECIPITATION; FOREST; MODIS; WATER; SOIL AB The Upper San Pedro River is one of the few remaining undammed rivers that maintain a vibrant riparian ecosystem in the southwest United States. However, its riparian forest is threatened by diminishing groundwater and surface water inputs, due to either changes in watershed characteristics such as changes in riparian and upland vegetation, or human activities such as regional groundwater pumping. We used satellite vegetation indices to quantify the green leaf density of the groundwater-dependent riparian forest from 1984 to 2012. The river was divided into a southern, upstream (mainly perennial flow) reach and a northern, downstream (mainly intermittent and ephemeral flow) reach. Pre-monsoon (June) Landsat normalized difference vegetation index (NDVI) values showed a 20% drop for the northern reach (P<0001) and no net change for the southern reach (P>005). NDVI and enhanced vegetation index values were positively correlated (P<005) with river flows, which decreased over the study period in the northern reach, and negatively correlated (P<005) with air temperatures in both reaches, which have increased by 14 degrees C from 1932 to 2012. NDVI in the uplands around the river did not increase from 1984 to 2012, suggesting that increased evapotranspiration in the uplands was not a factor in reducing river flows. Climate change, regional groundwater pumping, changes in the intensity of monsoon rain events and lack of overbank flooding are feasible explanations for deterioration of the riparian forest in the northern reach. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Uyen Nguyen; Glenn, Edward P.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. [Nagler, Pamela L.] US Geol Survey, Southwest Biol Sci Ctr, Sonoran Desert Res Stn, Tucson, AZ USA. [Scott, Russell L.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. RP Glenn, EP (reprint author), Univ Arizona, Dept Soil Water & Environm Sci, 1177 E 4th St,POB 210038, Tucson, AZ 85721 USA. EM eglenn@ag.arizona.edu NR 61 TC 3 Z9 3 U1 1 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD JUN PY 2015 VL 8 IS 4 BP 610 EP 625 DI 10.1002/eco.1529 PG 16 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA CL0JN UT WOS:000356628900007 ER PT J AU Vico, G Thompson, SE Manzoni, S Molini, A Albertson, JD Almeida-Cortez, JS Fay, PA Feng, X Guswa, AJ Liu, H Wilson, TG Porporato, A AF Vico, Giulia Thompson, Sally E. Manzoni, Stefano Molini, Annalisa Albertson, John D. Almeida-Cortez, Jarcilene S. Fay, Philip A. Feng, Xue Guswa, Andrew J. Liu, Hu Wilson, Tiffany G. Porporato, Amilcare TI Climatic, ecophysiological, and phenological controls on plant ecohydrological strategies in seasonally dry ecosystems SO ECOHYDROLOGY LA English DT Review DE seasonally dry ecosystem; Mediterranean climate; savanna; tropical dry forest; water stress; rainfall variability; soil moisture; carbon balance ID TROPICAL DECIDUOUS FOREST; NEOTROPICAL SAVANNA TREES; MEDITERRANEAN EVERGREEN OAKS; LEAF FUNCTIONAL TRAITS; VAPOR-PRESSURE DEFICIT; WATER RELATIONS; SOIL-WATER; RAINFALL SEASONALITY; GAS-EXCHANGE; SAP-FLOW AB Large areas in the tropics and at mid-latitudes experience pronounced seasonality and inter-annual variability in rainfall and hence water availability. Despite the importance of these seasonally dry ecosystems (SDEs) for the global carbon cycling and in providing ecosystem services, a unifying ecohydrological framework to interpret the effects of climatic variability on SDEs is still lacking. A synthesis of existing data about plant functional adaptations in SDEs, covering some 400 species, shows that leaf phenological variations, rather than physiological traits, provide the dominant control on plant-water-carbon interactions. Motivated by this result, the combined implications of leaf phenology and climatic variability on plant water use strategies are here explored with a minimalist model of the coupled soil water and plant carbon balances. The analyses are extended to five locations with different hydroclimatic forcing, spanning seasonally dry tropical climates (without temperature seasonality) and Mediterranean climates (exhibiting out of phase seasonal patterns of rainfall and temperature). The most beneficial leaf phenology in terms of carbon uptake depends on the climatic regime: evergreen species are favoured by short dry seasons or access to persistent water stores, whereas high inter-annual variability of rainy season duration favours the coexistence of multiple drought-deciduous phenological strategies. We conclude that drought-deciduousness may provide a competitive advantage in face of predicted declines in rainfall totals, while reduced seasonality and access to deep water stores may favour evergreen species. This article has been contributed to by US Government employees and their work is in the public domain in the USA. C1 [Vico, Giulia; Manzoni, Stefano] Swedish Univ Agr Sci SLU, Dept Crop Prod Ecol, Uppsala, Sweden. [Thompson, Sally E.] Univ Calif Berkeley, Dept Civil & Environm Engn, Berkeley, CA 94720 USA. [Manzoni, Stefano] Swedish Univ Agr Sci SLU, Dept Ecol, Uppsala, Sweden. [Manzoni, Stefano] Stockholm Univ, Dept Phys Geog & Quaternary Geol, S-10691 Stockholm, Sweden. [Molini, Annalisa] Masdar Inst Sci & Technol, iWater Ctr, Abu Dhabi, U Arab Emirates. [Molini, Annalisa] Masdar Inst Sci & Technol, Dept Chem & Environm Engn, Abu Dhabi, U Arab Emirates. [Albertson, John D.; Feng, Xue; Wilson, Tiffany G.; Porporato, Amilcare] Duke Univ, Dept Civil & Environm Engn, Durham, NC 27706 USA. [Albertson, John D.; Porporato, Amilcare] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Almeida-Cortez, Jarcilene S.] Univ Fed Pernambuco, Dept Bot, Recife, PE, Brazil. [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Guswa, Andrew J.] Smith Coll, Ctr Environm Ecol Design & Sustainabil, Northampton, MA 01063 USA. [Guswa, Andrew J.] Smith Coll, Picker Engn Program, Northampton, MA 01063 USA. [Liu, Hu] Chinese Acad Sci, Chinese Ecosyst Res Network, Linze Inland River Basin Res Stn, Lanzhou, Peoples R China. [Liu, Hu] Chinese Acad Sci, Key Lab Ecohydrol & River Basin Sci, Lanzhou, Peoples R China. [Liu, Hu] Penn State Univ, University Pk, PA 16802 USA. RP Vico, G (reprint author), Swedish Univ Agr Sci, Dept Crop Prod Ecol, POB 7043, SE-75007 Uppsala, Sweden. EM giulia.vico@slu.se RI Manzoni, Stefano/C-5330-2009; Vico, Giulia/A-6296-2010; Molini, Annalisa/E-8235-2016; Almeida-Cortez, Jarcilene/G-2935-2012; Thompson, Sally /J-7615-2012 OI Manzoni, Stefano/0000-0002-5960-5712; Vico, Giulia/0000-0002-7849-2653; Molini, Annalisa/0000-0003-3815-3929; Almeida-Cortez, Jarcilene/0000-0002-7118-7090; Thompson, Sally /0000-0003-4618-5066 FU US National Science Foundation [CBET 10-33467, FESD 1338694, DEB-1145875/1145649, EAR-1013339, EAR-1331940]; Pratt School of Engineering, Duke University; US Department of Agriculture [2011-67003-30222]; US Department of Energy through the Office of Biological and Environmental Research (BER) Terrestrial Ecosystem Science (TES) Program [DE-SC0006967]; project 'AgResource - Resource Allocation in Agriculture', from the Faculty of Natural Resources and Agricultural Sciences, Swedish University of Agricultural Sciences (SLU); Masdar Institute (One-to-One MIT-MI) [12WAMA1]; Masdar Institute [12WAMC1]; Masdar Institute Cooperative Program; USDA-NIFA [2010-65615-20632] FX This research was initiated at the 'Ecohydrology and Sustainability in Seasonally Dry Ecosystems' International Workshop (Duke University, 13-14 June 2011), which was supported by the US National Science Foundation, through grant CBET 10-33467, and by the Pratt School of Engineering, Duke University. We thank Ignacio Rodriguez-Iturbe for the stimulating discussions, and Kathrine Behrman, Virginia L. Jin, and two anonymous reviewers for their comments on earlier versions of the manuscript. The Instituto Fazenda Tamandua is also acknowledged as a catalyzer of collaboration across institutions and for providing information about the North-Eastern Brazil site. G.V., S.M., X.F., and A.P. gratefully acknowledge the support of the US National Science Foundation (CBET 10-33467, FESD 1338694, DEB-1145875/1145649), the US Department of Agriculture (2011-67003-30222), and the US Department of Energy through the Office of Biological and Environmental Research (BER) Terrestrial Ecosystem Science (TES) Program (DE-SC0006967). G.V. also acknowledges the support of the project 'AgResource - Resource Allocation in Agriculture', from the Faculty of Natural Resources and Agricultural Sciences, Swedish University of Agricultural Sciences (SLU). S.T. acknowledges the US National Science Foundation (EAR-1013339, EAR-1331940). A. M. acknowledges the support from the Masdar Institute (One-to-One MIT-MI, #12WAMA1 and Flagship #12WAMC1) in the framework of the MIT and Masdar Institute Cooperative Program. P.F. acknowledges support from USDA-NIFA (2010-65615-20632). A.P. also acknowledges the 'Lagrange fellow' project by the CRT Foundation and the Institute for Scientific Interchange. NR 157 TC 13 Z9 13 U1 13 U2 80 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD JUN PY 2015 VL 8 IS 4 BP 660 EP 681 DI 10.1002/eco.1533 PG 22 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA CL0JN UT WOS:000356628900011 ER PT J AU Gill, KA O'neal, ME AF Gill, K. A. O'neal, M. E. TI Survey of Soybean Insect Pollinators: Community Identification and Sampling Method Analysis SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE soybean; native bee; syrphid; Apis mellifera; pollen ID CORN-ROOTWORM COLEOPTERA; BEES HYMENOPTERA; PAN TRAPS; APIFORMES; LANDSCAPE; ABUNDANCE; DIPTERA; CROPS; ARTHROPODS; SYRPHIDAE AB Soybean, Glycine max (L.) Merrill, flowers can be a source of nectar and pollen for honey bees, Apis mellifera L. (Hymenoptera: Apidae), wild social and solitary bees (Hymenoptera: Apoidea), and flower-visiting flies (Diptera). Our objectives were to describe the pollinator community in soybean fields, determine which sampling method is most appropriate for characterizing their abundance and diversity, and gain insight into which pollinator taxa may contact soybean pollen. We compared modified pan traps (i.e., bee bowls), yellow sticky traps, and sweep nets for trapping pollinators in Iowa soybean fields when soybeans were blooming (i.e., reproductive stages R1-R6) during 2011 and 2012. When all trap type captures were combined, we collected 5,368 individuals and at least 50 species. Per trap type, the most pollinators were captured in bee bowls (3,644 individuals, 44 species), yellow sticky traps (1,652 individuals, 32 species), and sweep nets (66 individuals, 10 species). The most abundant species collected include Agapostemon virescens F. and Lasioglossum (Dialictus) species (Hymenoptera: Halictidae), Melissodes bimaculata Lepeletier (Hymenoptera: Apidae), and Toxomerus marginatus Say (Diptera: Syrphidae). To determine if these pollinators were foraging on soybean flowers, we looked for soybean pollen on the most abundant bee species collected that had visible pollen loads. We found soybean pollen alone or intermixed with pollen grains from other plant species on 29 and 38% of the bees examined in 2011 and 2012, respectively. Our data suggest a diverse community of pollinators-composed of mostly native, solitary bees-visit soybean fields and forage on their flowers within Iowa. C1 [Gill, K. A.] Nat Resources Conservat Serv, Xerces Soc Invertebrate Conservat, USDA, Columbus, NJ 08022 USA. [O'neal, M. E.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. RP O'neal, ME (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM oneal@iastate.edu FU DuPont Pioneer Hi-bred International; Leopold Center for Sustainable Agriculture FX We acknowledge funding to conduct this research from DuPont Pioneer Hi-bred International and the Leopold Center for Sustainable Agriculture. We had assistance with fieldwork and bee identification from Michael Joseph Wheelock and Kathryn Russell. NR 50 TC 6 Z9 7 U1 12 U2 50 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 488 EP 498 DI 10.1093/ee/nvv001 PG 11 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700006 PM 26313954 ER PT J AU Angelella, GM Egel, DS Holland, JD Nemacheck, JA Williams, CE Kaplan, I AF Angelella, G. M. Egel, D. S. Holland, J. D. Nemacheck, J. A. Williams, C. E. Kaplan, I. TI Differential Life History Trait Associations of Aphids with Nonpersistent Viruses in Cucurbits SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE watermelon mosaic virus; papaya ringspot virus; Aphis craccivora; Therioaphis trifolii; Aphis nerii ID CUCUMBER-MOSAIC-VIRUS; POTATO-LEAFROLL-VIRUS; PLANT-VIRUSES; SOUTHERN ILLINOIS; SECONDARY SPREAD; TOBACCO PLANTS; TRANSMISSION; VECTORS; EFFICIENCY; HOMOPTERA AB The diversity of vectors and fleeting nature of virus acquisition and transmission renders nonpersistent viruses a challenge to manage. We assessed the importance of noncolonizing versus colonizing vectors with a 2-yr survey of aphids and nonpersistent viruses on commercial pumpkin farms. We quantified aphid alightment using pan traps, while testing leaf samples with multiplex RT-PCR targeting cucumber mosaic virus (CMV), zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), and papaya ringspot virus (PRSV). Overall, we identified 53 aphid species (3,899 individuals), from which the melon aphid, Aphis gossypii Glover, a pumpkin-colonizing species, predominated (76 and 37% of samples in 2010 and 2011, respectively). CMV and ZYMV were not detected, but WMV and PRSV were prevalent, both regionally (WMV: 28/29 fields, PRSV: 21/29 fields) and within fields (infection rates = 69 and 55% for WMV in 2010 and 2011; 28 and 25% for PRSV in 2010 and 2011). However, early-season samples showed extremely low infection levels, suggesting cucurbit viruses are not seed-transmitted and implicating aphid activity as a causal factor driving virus spread. Interestingly, neither noncolonizer and colonizer alightment nor total aphid alightment were good predictors of virus presence, but community analyses revealed species-specific relationships. For example, cowpea aphid (Aphis craccivora Koch) and spotted alfalfa aphid (Therioaphis trifolii Monell f. maculata) were associated with PRSV infection, whereas the oleander aphid (Aphis nerii Bover de Fonscolombe) was associated with WMV spread within fields. These outcomes highlight the need for tailored management plans targeting key vectors of nonpersistent viruses in agricultural systems. C1 [Angelella, G. M.; Holland, J. D.; Kaplan, I.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Egel, D. S.] Southwest Purdue Agr Ctr, Vincennes, IN 47591 USA. [Nemacheck, J. A.; Williams, C. E.] Purdue Univ, USDA, ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Nemacheck, J. A.; Williams, C. E.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Angelella, GM (reprint author), Purdue Univ, Dept Entomol, 901 West State St, W Lafayette, IN 47907 USA. EM gangelel@purdue.edu FU United States Agricultural Service (USDA) North Central Region Integrated Pest Management (IPM) [11-34103-30723] FX We would like to thank Doris Lagos and David Voegtlin for their assistance with and training in aphid identification; the private pumpkin growers and the staff at Purdue University's Meigs Farm who gave permission to collect specimens from their fields; Christian Krupke for advice regarding aphid sampling; Shannon Pearce and Sara Hoke for their assistance collecting aphid and pumpkin leaf samples; Joseph Anderson, Shahideh Nouri, and Mahua Deb for their advice regarding RNA extraction and primer design; Carmen Blubaugh, Juli Carrillo, Michael Garvey, Ulianova Vidal Gomez, Laura Ingwell, Elizabeth Long, Paola Olaya, and Elizabeth Rowen for helpful comments on editing the manuscript; and the United States Agricultural Service (USDA) North Central Region Integrated Pest Management (IPM) Grants Program, grant 11-34103-30723, for funding this research. NR 67 TC 1 Z9 1 U1 3 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 562 EP 573 DI 10.1093/ee/nvv058 PG 12 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700013 PM 26313961 ER PT J AU Mustafa, T Horton, DR Swisher, KD Zack, RS Munyaneza, JE AF Mustafa, T. Horton, D. R. Swisher, K. D. Zack, R. S. Munyaneza, J. E. TI Effects of Host Plant on Development and Body Size of Three Haplotypes of Bactericera cockerelli (Hemiptera: Triozidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE potato psyllid haplotype; developmental trait; body size; potato; bittersweet nightshade ID ZEBRA CHIP DISEASE; CANDIDATUS LIBERIBACTER SOLANACEARUM; POTATO PSYLLID HAPLOTYPES; UNITED-STATES; LIFE-HISTORY; 1ST REPORT; HOMOPTERA PSYLLIDAE; FIELD CONDITIONS; TABLES; TOMATO AB Potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is an economic pest of solanaceous crops in North and Central America, and in New Zealand. Four genetic haplotypes of the psyllid have been identified in North America. Three of these haplotypes (Central, Western, and Northwestern) are common on potato crops within the major potato-growing regions of Idaho, Oregon, and Washington. Within this growing region, a weedy perennial nightshade, Solanum dulcamara (bittersweet nightshade), has been identified to be an important overwintering host and spring or summer source of psyllids colonizing potato fields. It is unclear whether bittersweet nightshade is a highly suitable host plant for all three haplotypes known to occur in the Pacific Northwest. The objective of the present study was to examine developmental traits and adult body size of all three haplotypes of psyllids reared on potato and bittersweet nightshade. Averaged over haplotype, development times were longer for psyllids reared on nightshade than potato. Duration of the preoviposition period, egg incubation requirements, nymphal development time, and total developmental time averaged 7.4, 5.9, 23.5, and 29.5 d on nightshade and 4.9, 5.5, 22.3, and 27.9 d on potato, respectively. The largest host effects were found for the Central haplotype, which exhibited a substantially extended (by over 5 d) preoviposition period on nightshade compared with potato. Averaged over host plant, nymphal and total development times of the Northwestern haplotype were longer (25.5 and 31.1 d, respectively) than those of the Western and Central haplotypes. The Northwestern haplotype was largest in overall body size, while the Central haplotype had the smallest overall body size, irrespective of host plant. Both sexes exhibited this trend. C1 [Mustafa, T.; Horton, D. R.; Swisher, K. D.; Munyaneza, J. E.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Mustafa, T.; Zack, R. S.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. RP Munyaneza, JE (reprint author), ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Joseph.Munyaneza@ars.usda.gov FU Northwest Potato Research Consortium; United States Department of Agriculture (USDA)-Agricultural Research Service State Cooperative Potato Research Program; USDA-National Institute of Food and Agriculture-Specialty Crop Research Initiative [2009-51181-20176] FX We thank Eugene Miliczky, Millie Heidt, Venkat Sengoda, and Francisco de la Rosa for their technical assistance. Financial support for this research was partially provided by the Northwest Potato Research Consortium, United States Department of Agriculture (USDA)-Agricultural Research Service State Cooperative Potato Research Program, and USDA-National Institute of Food and Agriculture-Specialty Crop Research Initiative (Project 2009-51181-20176). NR 31 TC 1 Z9 1 U1 2 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 593 EP 600 DI 10.1093/ee/nvv018 PG 8 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700017 PM 26313965 ER PT J AU Esquivel, JF AF Esquivel, J. F. TI Stylet Penetration Estimates for a Suite of Phytophagous Hemipteran Pests of Row Crops SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE stylet; feeding depth; rostrum length; Miridae; Pentatomidae ID NEZARA-VIRIDULA HEMIPTERA; GREEN STINK BUG; LYGUS-HESPERUS; COTTON; PENTATOMIDAE; TRANSMISSION; MIRIDAE; TEXAS; SPP.; L. AB Members of the Miridae (Lygus lineolaris Palisot de Beauvois and Pseudatomoscelis seriatus Reuter) and Pentatomidae (Acrosternum hilare Say, Euschistus servus (Say), Euschistus tristigmus (Say), Euschistus quadrator Rolston, Oebalus pugnax (F.), Piezodorus guildinii (Westwood), and Thyanta custator accerra McAtee) comprise a piercing-sucking insect complex that continues to plague multiple crops, including cotton. All these species have been associated with pathogen transmission. Breaching of boll carpel walls facilitates introduction of pathogens, and it is unknown whether stylets of these pests can fully penetrate carpel walls. Thus, stylet penetration estimates are needed and have been the focus of the present work. Stylet penetration estimates for L. lineolaris were significantly deeper than P. seriatus. Among the Pentatomidae, highest mean penetrationwas estimated for E. servus followed by A. hilare, yet A. hilare possessed a longer rostrum. Similarly, O. pugnax showed deeper penetration estimates than P. guildinii yet P. guildinii possessed a longer rostrum. Thus, rostrum length should not be equated to penetration potential. Pseudatomoscelis seriatus and L. lineolaris both infest early-season cotton, and the ranges of observed penetration indicate these insects, as well as the Pentatomidae, can breach the walls of critical pinhead squares and smaller bolls. The insects addressed herein affect a myriad of crops globally, and penetration estimates allow identification of growth stages susceptible to feeding and disease transmission. C1 ARS, USDA, Plains Area, Southern Plains Agr Res Ctr,Insect Control & Cott, College Stn, TX 77845 USA. RP Esquivel, JF (reprint author), ARS, USDA, Plains Area, Southern Plains Agr Res Ctr,Insect Control & Cott, 2765 F&B Rd, College Stn, TX 77845 USA. EM Jesus.Esquivel@ars.usda.gov NR 32 TC 1 Z9 1 U1 2 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 619 EP 626 DI 10.1093/ee/nvv027 PG 8 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700020 PM 26313968 ER PT J AU Funderburk, C Funderburk, J Tyler-Julian, K Srivastava, M Knox, G Andersen, P Adkins, S AF Funderburk, Charles Funderburk, Joe Tyler-Julian, Kara Srivastava, Mrittunjai Knox, Gary Andersen, Peter Adkins, Scott TI Population Dynamics of Frankliniella bispinosa (Thysanoptera: Thripidae) and the Predator Orius insidiosus (Hemiptera: Anthocoridae) as Influenced by Flower Color of Lagerstroemia (Lythraceae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Frankliniella tritici; Lagerstroemia indica; flower color; numerical response ID IN-FIELD PEPPER; STICKY TRAPS; SPANISH MOSS; OCCIDENTALIS THYSANOPTERA; INTEGRATED MANAGEMENT; SPECIES THYSANOPTERA; ABUNDANCE; FLORIDA; HOSTS; POLLEN AB Crapemyrtle is a common landscape planting that is a resource subsidy for beneficial insects. Field studies were conducted to determine the influence of crapemyrtle flower color on the population abundances and predator-prey dynamics of the herbivorous Frankliniella species and the predator Orius insidiosus. Adults and immatures of predator and prey were highly anthophilous, preferring white 'Acoma' flowers compared with lavender 'Apalachee', red 'Carolina Beauty', and pink 'Choctaw'. The predator was aggregated with its prey in a density-dependent manner: the adults by preferring the crapemyrtle clones also preferred by the thrips and the nymphs by direct tracking or as a function of increased prey and fecundity. Acoma was best for preference and buildup of O. insidiosus populations, and it was the only clone where there was no buildup in thrips populations. Two species of Karnyothrips (Thysanoptera: Phlaoethripidae), predators of small insects, were common in Tillandsia usneoides, an epiphyte on the crapemyrtle. Crapemyrtle is a bridge to enhance populations of O. insidiosus during summer months when there are few other hosts in the southern USA. C1 [Funderburk, Charles; Funderburk, Joe; Tyler-Julian, Kara; Srivastava, Mrittunjai; Knox, Gary; Andersen, Peter] Univ Florida, North Florida Res & Educ Ctr, Quincy, FL 32351 USA. [Adkins, Scott] ARS, Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. RP Funderburk, J (reprint author), Univ Florida, North Florida Res & Educ Ctr, 155 Res Rd, Quincy, FL 32351 USA. EM jef@ufl.edu FU University of Florida [58-6618-2-096]; United States Department of Agriculture, Agricultural Research Service FX We thank Russell Mizell III of the University of Florida for invaluable advice and assistance. The research was partially supported by Cooperative Agreement 58-6618-2-096 between the University of Florida and the United States Department of Agriculture, Agricultural Research Service. NR 43 TC 3 Z9 3 U1 2 U2 28 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 668 EP 679 DI 10.1093/ee/nvv057 PG 12 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700025 PM 26313973 ER PT J AU Manoukis, NC Siderhurst, M Jang, EB AF Manoukis, Nicholas C. Siderhurst, Matthew Jang, Eric B. TI Field Estimates of Attraction of Ceratitis capitata to Trimedlure and Bactrocera dorsalis (Diptera: Tephritidae) to Methyl Eugenol in Varying Environments SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE mark-release-recapture; pheromone; attraction range; tephritid ID MEDITERRANEAN FRUIT-FLY; EFFECTIVE SAMPLING RANGE; STERILE INSECT TECHNIQUE; FLIES DIPTERA; PHEROMONE TRAPS; RELEASED MALES; DISPERSAL; FEMALE; CALIFORNIA; DISTANCE AB Measuring and modeling the attractiveness of semiochemical-baited traps is of significant importance to detection, delimitation, and control of invasive pests. Here, we describe the results of field mark-release-recapture experiments with Ceratitis capitata (Wiedemann) and Bactrocera dorsalis (Hendel) to estimate the relationship between distance from a trap baited with trimedlure and methyl eugenol, respectively, and probability of capture for a receptive male insect. Experiments were conducted using a grid of traps with a central release point at two sites on Hawaii Island, a Macadamia orchard on the East side of the island and a lava field on theWest side. We found that for B. dorsalis and methyl eugenol there is a 65% probability of capture at similar to 36 m from a single trap, regardless of habitat. For C. capitata, we found a 65% probability of capture at a distance of similar to 14 m from a single trap in the orchard and 7 m in the lava field. We also present results on the spatial and temporal pattern of recaptures. The attraction data are analyzed via a hyperbolic secant-based capture probability model. C1 [Manoukis, Nicholas C.; Jang, Eric B.] ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Siderhurst, Matthew] Eastern Mennonite Univ, Dept Chem, Harrisonburg, VA 22802 USA. RP Manoukis, NC (reprint author), ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM nicholas.manoukis@ars.usda.gov OI Manoukis, Nicholas/0000-0001-5062-7256 FU U.S. Department of Agriculture-Agricultural Research Services (USDA-ARS); California Department of Food and Agriculture (CDFA); International Atomic Energy Agency Technical [18462/R0] FX We would like to thank Stephanie Gayle, Thomas Mangine, Andrew Yoshimoto, and others at DKI-PBARC for assistance in conducting the experiments. Thanks also to Mike McKenney and Keith Shigetani for providing flies for these experiments from the DKI-PBARC insect colony. We thank the Dean Takebayashi and the State of Hawaii, Department of Land and Natural Resources for permission to work in Pu'u waawaa, as well as Island Princess for permission to work in their Macadamia orchard in Keaau. We are also grateful to Walther Enkerlin and Roger Vargas for constructive comments on an early draft of the manuscript, and to three anonymous referees for their reviews. This work was funded by U.S. Department of Agriculture-Agricultural Research Services (USDA-ARS), the California Department of Food and Agriculture (CDFA), and International Atomic Energy Agency Technical Contract No. 18462/R0. Opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. USDA is an equal opportunity provider and employer. NR 55 TC 1 Z9 1 U1 6 U2 25 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 695 EP 703 DI 10.1093/ee/nvv020 PG 9 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700028 PM 26313976 ER PT J AU Burrack, HJ Asplen, M Bahder, L Collins, J Drummond, FA Guedot, C Isaacs, R Johnson, D Blanton, A Lee, JC Loeb, G Rodriguez-Saona, C van Timmeren, S Walsh, D McPhie, DR AF Burrack, Hannah J. Asplen, Mark Bahder, Luz Collins, Judith Drummond, Francis A. Guedot, Christelle Isaacs, Rufus Johnson, Donn Blanton, Anna Lee, Jana C. Loeb, Gregory Rodriguez-Saona, Cesar van Timmeren, Steven Walsh, Douglas McPhie, Douglas R. TI Multistate Comparison of Attractants for Monitoring Drosophila suzukii (Diptera: Drosophilidae) in Blueberries and Caneberries SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE insect monitoring; fermentation bait; integrated pest management; invasive species ID SPOTTED-WING DROSOPHILA; INTEGRATED PEST-MANAGEMENT; PHEROMONE LURES; TRAPS; COMBINATIONS; INSECTICIDES; CAPTURES; VINEGAR; CROPS; FIELD AB Drosophila suzukii Matsumara, also referred to as the spotted wing drosophila, has recently expanded its global range with significant consequences for its primary host crops: blueberries, blackberries, raspberries, cherries, and strawberries. D. suzukii populations can increase quickly, and their infestation is difficult to predict and prevent. The development of effective tools to detect D. suzukii presence in new areas, to time the beginning of activity within a crop, to track seasonal activity patterns, and to gauge the effectiveness of management efforts has been a key research goal. We compared the efficiency, selectivity, and relationship to fruit infestation of a range of commonly used homemade baits and a synthetic formulated lure across a wide range of environments in 10 locations throughout the United States. Several homemade baits were more efficient than apple cider vinegar, a commonly used standard, and a commercially formulated lure was, in some configurations and environments, comparable with the most effective homemade attractant as well as potentially more selective. All alternative attractants also captured flies between 1 and 2 wk earlier than apple cider vinegar, and detected the presence of D. suzukii prior to the development of fruit infestation. Over half the Drosophila spp. flies captured in traps baited with any of the attractants were not D. suzukii, which may complicate their adoption by nonexpert users. The alternative D. suzukii attractants tested are improvement on apple cider vinegar and may be useful in the development of future synthetic lures. C1 [Burrack, Hannah J.; McPhie, Douglas R.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Asplen, Mark] Metropolitan State Univ, Coll Arts & Sci, Dept Nat Sci, St Paul, MN 55106 USA. [Bahder, Luz; Walsh, Douglas] Washington State Univ, Dept Entomol, Prosser, WA 99350 USA. [Collins, Judith; Drummond, Francis A.] Univ Maine, Sch Biol & Ecol, Orono, ME 04469 USA. [Guedot, Christelle] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Isaacs, Rufus; van Timmeren, Steven] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Johnson, Donn] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. [Blanton, Anna] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Lee, Jana C.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Loeb, Gregory] Cornell Univ, Dept Entomol, Geneva Expt Stn, Geneva, NY 14456 USA. [Rodriguez-Saona, Cesar] Rutgers State Univ, Dept Entomol, Chatsworth, NJ 08019 USA. RP Burrack, HJ (reprint author), N Carolina State Univ, Dept Entomol, Campus Box 7634, Raleigh, NC 27695 USA. EM hjburrac@ncsu.edu FU U.S. Department of Agriculture Specialty Crop Research Initiative (USDA SCRI) [2010-51181-21167]; U.S. Department of Agriculture Current Research Information System (USDA CRIS) [5358-22000-037-00D]; USDA/Agricultural Marketing Service (AMS)/Arkansas Agriculture Department SCRI [12-25-B-1214]; USDA/National Institute of Food and Agriculture (NIFA)/University of Arkansas EIPM Coordination Program Grant FX We thank Robert Holdcraft, Bridget Blood, Alison Denelsbeck, Jennifer Frake, Christina Fieland, Jesse Mindolovich, Tressa Franzmeier, Chris Pontius, Barbara Lewis, and Taunya Ernst for field and laboratory assistance. Funding was provided by the U.S. Department of Agriculture Specialty Crop Research Initiative (USDA SCRI) grant 2010-51181-21167, U.S. Department of Agriculture Current Research Information System (USDA CRIS) 5358-22000-037-00D, USDA/Agricultural Marketing Service (AMS)/Arkansas Agriculture Department SCRI Grant 12-25-B-1214, USDA/National Institute of Food and Agriculture (NIFA)/University of Arkansas EIPM Coordination Program Grant. We are grateful to the fruit growers that allowed us to set up our experiments on their farms. NR 30 TC 15 Z9 15 U1 7 U2 64 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 704 EP 712 DI 10.1093/ee/nvv022 PG 9 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700029 PM 26313977 ER PT J AU Leskey, TC Agnello, A Bergh, JC Dively, GP Hamilton, GC Jentsch, P Khrimian, A Krawczyk, G Kuhar, TP Lee, DH Morrison, WR Polk, DF Rodriguez-Saona, C Shearer, PW Short, BD Shrewsbury, PM Walgenbach, JF Weber, DC Welty, C Whalen, J Wiman, N Zaman, F AF Leskey, Tracy C. Agnello, Arthur Bergh, J. Christopher Dively, Galen P. Hamilton, George C. Jentsch, Peter Khrimian, Ashot Krawczyk, Grzegorz Kuhar, Thomas P. Lee, Doo-Hyung Morrison, William R., III Polk, Dean F. Rodriguez-Saona, Cesar Shearer, Peter W. Short, Brent D. Shrewsbury, Paula M. Walgenbach, James F. Weber, Donald C. Welty, Celeste Whalen, Joanne Wiman, Nik Zaman, Faruque TI Attraction of the Invasive Halyomorpha halys (Hemiptera: Pentatomidae) to Traps Baited with Semiochemical Stimuli Across the United States SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE brown marmorated stink bug; pheromone; monitoring; IPM; traps ID MARMORATED STINK BUG; LIFE TABLE ANALYSIS; AGGREGATION PHEROMONE; STAL HETEROPTERA; TREE FRUIT; IMPACT; INSECTICIDES; PHENOLOGY; ORCHARDS; FIELDS AB A recent identification of the two-component aggregation pheromone of the invasive stink bug species, Halyomorpha halys (Stal), in association with a synergist, has greatly improved the ability to accurately monitor the seasonal abundance and distribution of this destructive pest. We evaluated the attraction of H. halys to black pyramid traps baited with lures containing the pheromone alone, the synergist methyl (2E, 4E, 6Z)-decatrienoate (MDT) alone, and the two lures in combination. Traps were deployed around areas of agricultural production including fruit orchards, vegetables, ornamentals, or row crops in Delaware, Maryland, North Carolina, New Jersey, New York, Ohio, Oregon, Pennsylvania, Virginia, and West Virginia from mid-April to mid-October, 2012 and 2013. We confirmed that H. halys adults and nymphs are attracted to the aggregation pheromone season long, but that attraction is significantly increased with the addition of the synergist MDT. H. halys adults were detected in April with peak captures of overwintering adults in mid-to late May. The largest adult captures were late in the summer, typically in early September. Nymphal captures began in late May and continued season long. Total captures declined rapidly in autumn and ceased by mid-October. Captures were greatest at locations in the Eastern Inland region, followed by those in the Eastern Coastal Plain and Pacific Northwest. Importantly, regardless of location in the United States, all mobile life stages of H. halys consistently responded to the combination of H. halys aggregation pheromone and the synergist throughout the entire the entire season, suggesting that these stimuli will be useful tools to monitor for H. halys in managed systems. C1 [Leskey, Tracy C.; Morrison, William R., III; Short, Brent D.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Agnello, Arthur] Cornell Univ, Dept Entomol, Geneva, NY USA. [Bergh, J. Christopher] Virginia Tech, Dept Entomol, AHS AREC, Winchester, Hants, England. [Dively, Galen P.; Shrewsbury, Paula M.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Hamilton, George C.; Rodriguez-Saona, Cesar] Rutgers State Univ, Dept Entomol, New Brunswick, NJ 08903 USA. [Jentsch, Peter] Cornell Univ, Dept Entomol, Hudson Valley Res Lab, Highland, NY USA. [Khrimian, Ashot; Weber, Donald C.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD USA. [Krawczyk, Grzegorz] Penn State Univ, Dept Entomol, FREC, Biglerville, PA USA. [Kuhar, Thomas P.] Virginia Tech, Dept Entomol, Blacksburg, VA USA. [Lee, Doo-Hyung] Gachon Univ, Dept Life Sci, Songnam, Kyeonggi Do, South Korea. [Polk, Dean F.] Rutgers State Univ, Dept Agr & Resource Management, New Brunswick, NJ 08903 USA. [Shearer, Peter W.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, Hood River, OR 97031 USA. [Walgenbach, James F.] N Carolina State Univ, Dept Entomol, MHCREC, Mills River, NC USA. [Welty, Celeste] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA. [Whalen, Joanne] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE USA. [Wiman, Nik] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Zaman, Faruque] Cornell Cooperat Extens, Riverhead, NY USA. RP Leskey, TC (reprint author), ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. EM tracy.leskey@ars.usda.gov OI Morrison, William/0000-0002-1663-8741 FU U.S. Department of Agriculture- National Institute of Food and Agriculture-Specialty Crop Research Initiative (USDA-NIFA-SCRI) [2011-51181-30937] FX We would like to thank Amy Blood, Bridget Blood, Samuel Brandt, John Cullum, Allison Denelsbeck, Jean Engelman, Travis Enyeart, Elizabeth Fread, Torri Hancock, Nancy Harding, Robert Holdcraft, Erika Maslen, Zachary Moore, Cameron Scorza, Steve Schoof, and Sean Wiles for their excellent assistance in the field. This work was supported by U.S. Department of Agriculture- National Institute of Food and Agriculture-Specialty Crop Research Initiative (USDA-NIFA-SCRI) #2011-51181-30937. NR 31 TC 12 Z9 12 U1 5 U2 29 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 746 EP 756 DI 10.1093/ee/nvv049 PG 11 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700033 PM 26313981 ER PT J AU Cooper, WR Spurgeon, DW AF Cooper, W. Rodney Spurgeon, Dale W. TI Temperature-Dependent Survival of Adult Lygus hesperus (Hemiptera: Miridae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE western tarnished plant bug; Lygus hesperus; survival; temperature ID EGG AB The western tarnished plant bug, Lygus hesperus Knight (Hemiptera: Miridae), is a key pest of many horticultural and agronomic crops in the western United States. Despite its well documented pest status, many aspects of the basic biology, including overwintering ecology, of L. hesperus are poorly understood. We examined the influence of eight constant temperatures from 10 to 35 degrees C on survival of nondiapausing adult L. hesperus held with or without food, and the consequences of exposure to an extended period at 10 degrees C on subsequent reproduction. Survival analyses indicated that, on average, fed insects tended to live longer than unfed insects, females lived longer than males, and the survival time decreased with increasing temperature. Nonlinear regressions indicated that median survival for insects grouped by gender and feeding status declined exponentially with increasing temperature. Survival functions for combinations of insect class (gender and feeding status) and temperature were adequately described by the respective two-parameter logistic functions. When adults were held for 9 d at 27 degrees C with food after a 33-d period at 10 degrees C either with or without food, no deleterious effects of prior starvation on propensity to mate or fecundity were demonstrated. These findings indicate that when temperatures are low, nondiapausing L. hesperus adults are capable of extended host-free survival with little or no impact on subsequent reproduction. Our findings suggest the current understanding of L. hesperus overwintering dynamics is incomplete. In addition, our results provide quantitative baseline information to facilitate more comprehensive investigation of the ecology of L. hesperus overwintering. C1 [Cooper, W. Rodney; Spurgeon, Dale W.] ARS, USDA, Shafter Cotton Res Stn, Shafter, CA 93263 USA. RP Cooper, WR (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 NR 21 TC 3 Z9 3 U1 0 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 808 EP 813 DI 10.1093/ee/nvv015 PG 6 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700039 PM 26313987 ER PT J AU Demkovich, M Siegel, JP Higbee, BS Berenbaum, MR AF Demkovich, Mark Siegel, Joel P. Higbee, Bradley S. Berenbaum, May R. TI Mechanism of Resistance Acquisition and Potential Associated Fitness Costs in Amyelois transitella (Lepidoptera: Pyralidae) Exposed to Pyrethroid Insecticides SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Amyelois transitella; detoxification; insecticide; resistance; pyrethroid ID NAVEL ORANGEWORM; BODY-SIZE; ALMOND; XENOBIOTICS; TORTRICIDAE; MYCOTOXINS; PISTACHIOS; SELECTION; SURVIVAL AB The polyphagous navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), is the most destructive pest of nut crops, including almonds and pistachios, in California orchards. Management of this insect has typically been a combination of cultural controls and insecticide use, with the latter increasing substantially along with the value of these commodities. Possibly associated with increased insecticide use, resistance has been observed recently in navel orangeworm populations in Kern County, California. In studies characterizing a putatively pyrethroid-resistant strain (R347) of navel orangeworm, susceptibility to bifenthrin and beta-cyfluthrin was compared with that of an established colony of susceptible navel orangeworm. Administration of piperonyl butoxide and S,S,S-tributyl phosphorotrithioate in first-instar feeding bioassays with the pyrethroids bifenthrin and beta-cyfluthrin produced synergistic effects and demonstrated that cytochrome P450 monooxygenases and carboxylesterases contribute to resistance in this population. Resistance is therefore primarily metabolic and likely the result of overexpression of specific cytochrome P450 monooxygenases and carboxylesterase genes. Resistance was assessed by median lethal concentration (LC50) assays and maintained across nine generations in the laboratory. Life history trait comparisons between the resistant strain and susceptible strain revealed significantly lower pupal weights in resistant individuals reared on the same wheat bran-based artificial diet across six generations. Time to second instar was greater in the resistant strain than the susceptible strain, although overall development time was not significantly different between strains. Resistance was heritable and may have an associated fitness cost, which could influence the dispersal and expansion of resistant populations in nut-growing areas in California. C1 [Demkovich, Mark; Berenbaum, May R.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Siegel, Joel P.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Higbee, Bradley S.] Paramount Farming Co, Shafter, CA 93263 USA. RP Demkovich, M (reprint author), Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. EM mdemkov3@illinois.edu FU Almond Board of California; California Pistachio Research Board FX We thank the Almond Board of California and the California Pistachio Research Board for funding this research. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 46 TC 3 Z9 4 U1 3 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 855 EP 863 DI 10.1093/ee/nvv047 PG 9 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700044 PM 26313992 ER PT J AU Arango, RA Marschalek, DA Green, F Raffa, KF Berres, ME AF Arango, R. A. Marschalek, D. A. Green, F., III Raffa, K. F. Berres, M. E. TI Genetic Analysis of Termite Colonies in Wisconsin SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Reticulitermes flavipes (Kollar); Reticulitermes tibialis Banks; amplified fragment-length polymorphism; GENELAND; genetic population structure ID EASTERN SUBTERRANEAN TERMITE; RUN LENGTH CONTROL; RETICULITERMES-FLAVIPES; POPULATION-STRUCTURE; AFLP MARKERS; ISOPTERA; RHINOTERMITIDAE; COPTOTERMES; CANADA; DNA AB The objective of this study was to document current areas of subterranean termite activity in Wisconsin and to evaluate genetic characteristics of these northern, peripheral colonies. Here, amplified fragment-length polymorphism was used to characterize levels of inbreeding, expected heterozygosity, and percent polymorphism within colonies as well as genetic structure among populations sampled. Genetic analysis revealed two species of termites occur in Wisconsin, Reticulitermes flavipes (Kollar) and Reticulitermes tibialis Banks, both found in the southern half of the state. Colonies of both species in Wisconsin are thought to represent the northern boundary of their current distributions. Measurements of within colony genetic variation showed the proportion of polymorphic loci to be between 52.9-63.9% and expected heterozygosity to range from 0.122-0.189. Consistent with geographical isolation, strong intercolony genetic differences were observed, with over 50% of F-ST values above 0.25 and the remaining showing moderate levels of genetic differentiation. Combined with low levels of inbreeding in most collection locations (F-IS 0.042-0.123), we hypothesize termites were introduced numerous times in the state, likely by anthropogenic means. We discuss the potential effects of these genetic characteristics on successful colony establishment of termites along the northern boundary compared with termites in the core region of their distribution. C1 [Arango, R. A.; Green, F., III] USDA, Forest Prod Lab, Madison, WI 53726 USA. [Arango, R. A.; Marschalek, D. A.; Raffa, K. F.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Marschalek, D. A.] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA. [Berres, M. E.] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA. RP Arango, RA (reprint author), USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM rarango@fs.fed.us NR 36 TC 0 Z9 0 U1 5 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 890 EP 897 DI 10.1093/ee/nvv023 PG 8 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700048 PM 26313996 ER PT J AU Fleming, DE Roehrdanz, RL Allen, KC Musser, FR AF Fleming, D. E. Roehrdanz, R. L. Allen, K. C. Musser, F. R. TI Comparisons of Lygus lineolaris (Hemiptera: Miridae) Populations from Two Distinct Geographical Regions of Mississippi SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Lygus lineolaris; tarnished plant bug; landscape ecology; mtDNA ID PLANT BUG HEMIPTERA; AGRICULTURAL LANDSCAPE; MAXIMUM-LIKELIHOOD; MITOCHONDRIAL-DNA; HETEROPTERA; DIVERSITY; COTTON; DELTA; LEPIDOPTERA; NOCTUIDAE AB The tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), is a major pest of cotton (Gossypium hirsutum L.) in the state of Mississippi. Economic data indicate that L. lineolaris is a more serious pest of cotton in the Delta region of Mississippi than in the Hills region; however, little data exist comparing the two populations. Two experiments were undertaken to compare L. lineolaris from these two geographically distinct regions. In the first experiment, colonies of L. lineolaris from each region were reared in the laboratory under controlled conditions and measurements of development time, survivorship, fecundity, and hatch rate were compared. The geographic region of origin had no effect on any of the variables measured; however, the diet used for rearing had a significant effect on all variables except hatch rate. In the second experiment, part of the cox1 gene of the L. lineolaris mitochondrial genome was compared between the two populations to examine possible genetic differences between L. lineolaris from the two regions of Mississippi. Data revealed two cox1 clades in the Delta region and only one cox1 clade in the Hills region. Taken together, the data do not explain the reason for the differences in the severity of damage to cotton in the two regions. C1 [Fleming, D. E.; Musser, F. R.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Roehrdanz, R. L.] USDA ARS, Biosci Res Lab, Fargo, ND 58102 USA. [Allen, K. C.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. RP Fleming, DE (reprint author), Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Box 9775, Mississippi State, MS 39762 USA. EM def18@msstate.edu FU United States Department of Agriculture-Agricultural Research Service Specific Cooperative Agreement [58-6402-3-017] FX Thanks to Kata Grala of the MSU Geosciences department for the help in providing GIS data used to describe landscape differences of the two regions. Thanks to Kathy Knighten and the many student workers who maintained the rearing facilities and colonies. Thanks to Sheila Sears Wichmann for technical assistance in preparing DNA for analysis. Partial funding for this research was provided by United States Department of Agriculture-Agricultural Research Service Specific Cooperative Agreement 58-6402-3-017. NR 34 TC 2 Z9 2 U1 1 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2015 VL 44 IS 3 BP 898 EP 906 DI 10.1093/ee/nvv025 PG 9 WC Entomology SC Entomology GA CK4HA UT WOS:000356183700049 PM 26313997 ER PT J AU Kiobia, DO Tumbo, SD Cantillo, J Rohde, BB Mallikarjunan, PK Mankin, RW AF Kiobia, D. O. Tumbo, S. D. Cantillo, J. Rohde, B. B. Mallikarjunan, P. K. Mankin, R. W. TI Characterization of sounds in maize produced by internally feeding insects: investigations to develop inexpensive devices for detection of Prostephanus truncatus (Coleoptera: Bostrichidae) and Sitophilus zeamais (Coleoptera: Curculionidae) in small-scale storage facilities in sub-Saharan Africa SO FLORIDA ENTOMOLOGIST LA English DT Article DE acoustic probe; post-harvest; hidden infestation; grain; signal processing; Tanzania ID STORED-PRODUCT; HORN COLEOPTERA; GRAIN; RESPONSES; SIGNALS; WEEVIL; SYSTEM; PESTS AB Infestations by Prostephanus truncatus Horn (Coleoptera: Bostrichidae) and Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) are prevalent in small-scale Zea mays L. storage facilities in Tanzania and other regions of sub-Saharan Africa. It is especially difficult to detect these species' larvae, which feed unseen inside the grain kernels. An electronic device that acoustically detects and reliably indicates the presence of such larvae could assist pest managers in maintaining the quality of the stored maize. A study was conducted in a sound-and vibration-controlled environment to estimate the amplitudes and spectral ranges of signals that an inexpensive electronic system would encounter while detecting insects in maize storage facilities. Larva-infested wheat kernels from a laboratory colony of Sitophilus oryzae (L.), a species similar in size and behavior to S. zeamais, were placed in a pouch and inserted near the side or the bottom of a bag of maize. An acoustic probe was inserted into the bag, and recordings were made at multiple positions, 5-35 cm from the pouch. Numerous sounds of 4 different types were detected over a range of frequencies extending to 7 kHz, well within the signal-processing capabilities of currently available low-cost microcontroller platforms. Larval sound impulses were detected frequently within 25 cm from the pouch, but not at 35 cm. However, adjustable-length probes could be used to reach within 30 cm of all maize kernels in the types of containers commonly used in regional storage facilities. Thus, there is considerable potential to develop an inexpensive sensor/ microcontroller system useful for managing stored product insect pests in sub-Saharan Africa. C1 [Kiobia, D. O.; Mallikarjunan, P. K.] Virginia Tech Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA. [Tumbo, S. D.] Sokoine Univ Agr, Dept Agr Engn & Land Planning, Morogoro, Tanzania. [Cantillo, J.; Mankin, R. W.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Rohde, B. B.] Univ Florida, Dept Elect & Comp Engn, Gainesville, FL 32611 USA. RP Mankin, RW (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM richard.mankin@ars.usda.gov FU USAID iAGRI program; Citrus Research and Development Foundation; National Science Foundation Graduate Research Fellowship [DGE-1315138] FX We thank Betty Weaver for providing the S. oryzae larvae used in this study as well as Everett Foreman for setting up the acoustic detection equipment (USDA-ARS-CMAVE). This research was supported in part by grants from the USAID iAGRI program, the Citrus Research and Development Foundation, and National Science Foundation Graduate Research Fellowship DGE-1315138. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA of any product or service to the exclusion of others that may be suitable. The USDA is an equal opportunity provider and employer. NR 29 TC 1 Z9 1 U1 3 U2 17 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 405 EP 409 PG 5 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400002 ER PT J AU Wu, FN Cen, YJ Deng, XL Chen, JC Xia, YL Liang, GW AF Wu, Fengnian Cen, Yijing Deng, Xiaoling Chen, Jianchi Xia, Yulu Liang, Guangwen TI Movement of Diaphorina citri (Hemiptera: Liviidae) adults between huanglongbing-infected and healthy citrus SO FLORIDA ENTOMOLOGIST LA English DT Article DE Asian citrus psyllid; citrus greening disease; preference; infected mature-yellow leaves; physiologically mature-yellow leaves ID CANDIDATUS LIBERIBACTER ASIATICUS; GREENING DISEASE; SAO-PAULO; PSYLLIDAE; KUWAYAMA; VOLATILES; TRANSMISSION; HOMOPTERA; BACTERIUM; RESPONSES AB Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is the vector of huanglongbing (HLB or citrus greening disease). Preferences of D. citri adults differed for HLB-infected and healthy citrus under different maturity conditions. The presence or absence of young shoots had a significant influence on the choice by D. citri adults between HLB-infected and healthy citrus hosts. When citrus plants had young shoots, infected plants were more attractive than healthy ones to the adults. Also, D. citri adults fed for a longer time on infected plants with young shoots than on their healthy counterparts. In the absence of young shoots, D. citri adults were at first also more attracted to infected mature leaves, but after 38 h they turned to healthy mature leaves. In a multiple choice experiment, infected young shoots and healthy young shoots were the most attractive, followed by shoots with infected mature-yellow leaves and physiologically mature-yellow leaves, and lastly by healthy or infected mature-green leaves. In an experiment to measure the relative attractiveness of yellow, green, and white boards, yellow color boards attracted more adults than green and white boards, indicating that the adults preferred the yellow color. The results suggest that D. citri adults when first confronted with a choice are more attracted to infected citrus because of the color, but subsequently they move to healthy citrus perhaps because of either the poor nutrition or a feeding barrier in the infected hosts. This behavior appears to facilitate the pathogen's spread. C1 [Wu, Fengnian; Cen, Yijing; Deng, Xiaoling; Liang, Guangwen] South China Agr Univ, Citrus Huanglongbing Res Lab, Lab Insect Ecol, Guangzhou 510642, Guangdong, Peoples R China. [Chen, Jianchi] USDA ARS, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res, Parlier, CA 93648 USA. [Xia, Yulu] N Carolina State Univ, Ctr Integrated Pest Management, Raleigh, NC 27606 USA. RP Cen, YJ (reprint author), South China Agr Univ, Citrus Huanglongbing Res Lab, Lab Insect Ecol, Guangzhou 510642, Guangdong, Peoples R China. EM cenyj@scau.edu.cn; gwliang@scau.edu.cn FU Ministry of Agriculture of the People's Republic of China under the public beneficial industry project "Study and Demonstration of the Technology of Integrated Management of Citrus Huanglongbing and Canker" [201003067]; Guangdong Provincial Department of Science and Technology project "Study on Technology Integration of Citrus Huanglongbing Management Strategies and Demonstration in Guangdong Province" [2012A020200016]; USDA APHIS-North Carolina State University joint project entitled as "Asian Citrus Psyllid and Huanglongbing Field Research and Outreach." FX This study was funded by the Ministry of Agriculture of the People's Republic of China under the public beneficial industry project "Study and Demonstration of the Technology of Integrated Management of Citrus Huanglongbing and Canker" (201003067), the Guangdong Provincial Department of Science and Technology project "Study on Technology Integration of Citrus Huanglongbing Management Strategies and Demonstration in Guangdong Province" (2012A020200016), and a USDA APHIS-North Carolina State University joint project entitled as "Asian Citrus Psyllid and Huanglongbing Field Research and Outreach." NR 32 TC 1 Z9 3 U1 3 U2 38 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 410 EP 416 PG 7 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400003 ER PT J AU Dickey, AM Stocks, IC Smith, T Osborne, L McKenzie, CL AF Dickey, Aaron M. Stocks, Ian C. Smith, Trevor Osborne, Lance McKenzie, Cindy L. TI DNA barcode development for three recent exotic whitefly (Hemiptera: Aleyrodidae) invaders in Florida SO FLORIDA ENTOMOLOGIST LA English DT Article DE coxI barcoding; invasive species; Singhiella simplex; Aleurodicus rugioperculatus; Paraleyrodes bondari; Paraleyrodes pseudonaranjae ID CHAIN-REACTION PRIMERS; MAXIMUM-LIKELIHOOD; BEMISIA-TABACI; PHYLOGENETIC ANALYSIS; IDENTIFICATION GUIDE; MITOCHONDRIAL-DNA; UNITED-STATES; HOMOPTERA; STERNORRHYNCHA; UNIVERSAL AB Several new whitefly (Hemiptera: Aleyrodidae) species have become established in Florida in the past decade. Three of these, fig whitefly (FW), rugose spiraling whitefly (RSW), and Bondar's nesting whitefly (BNW), have caused noticeable damage to residential plants in the landscape including ficus hedges, palms, and bird of paradise. Whiteflies are difficult to identify and 4th instar nymphs are needed for morphological identification making whiteflies good candidates for identification via DNA barcoding. A DNA barcoding cocktail to amplify the 5' end of the coxI mitochondrial gene from these species was developed. Subsequently, primers were developed for each species, validated with multiple populations collected throughout Florida, and a phylogenetic tree was constructed for placement of the 3 species in the whitefly tree of life. Besides FW, RSW, and BNW, 2 additional species of whiteflies were detected in collections, namely Paraleyrodes pseudonaranjae Martin (Hemiptera: Aleyrodidae) and a species provisionally designated Aleurodicinae sp1. RSW and BNW clustered with congeners within the phylogeny, and FW was resolved as a possible sister taxa to the genus Bemisia. The barcoding cocktail should allow sequencing of 5' coxI from multiple genera and both sub-families of whiteflies, and the primers developed for each species will facilitate rapid identification of these 3 invasive whiteflies. C1 [Dickey, Aaron M.; Osborne, Lance] Univ Florida, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. [Stocks, Ian C.; Smith, Trevor] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. [McKenzie, Cindy L.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP McKenzie, CL (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM Cindy.McKenzie@ars.usda.gov NR 43 TC 0 Z9 1 U1 4 U2 10 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 473 EP 478 PG 6 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400013 ER PT J AU Nalen, CMZ Kline, DL Sutton, BD Muller, G Cilek, JE AF Nalen, Catherine M. Zettel Kline, Daniel L. Sutton, Bruce D. Mueller, Guenter Cilek, James E. TI An annotated checklist of the horse flies, deer flies, and yellow flies (Diptera: Tabanidae) of Florida SO FLORIDA ENTOMOLOGIST LA English DT Article DE tabanid; Tabanus; Chrysops; Chlorotabanus; Hybomitra; Agkistrocerus; Merycomyia; Microtabanus; Stenotabanus; Whitneyomyia ID UNITED-STATES; DISEASE AGENTS; TRANSMISSION; EMPHASIS AB The last compilation of the Tabanidae of Florida was published in 1964 by Calvin Jones & Darrell Anthony. Since then, several taxonomic and distributional changes have been made, as well as the addition of several state locality records. We have compiled a list of Tabanidae species currently present in the state of Florida, or potentially present in Florida based on surrounding state records, using literature surveys and personal examinations of the collections at the Florida State Collection of Arthropods, Gainesville, Florida, USA. Currently, 124 species/subspecies are recorded from Florida, with an additional 15 species with likely Florida distributions from 15 different genera. In contrast, Jones & Anthony (1964) recognized 118 species among 14 genera. Although the number of species is not vastly different, much taxonomic revision has been done to the Florida Tabanidae. Three new Florida records are presented for Tabanus reinwardtii Weidemann, 1828, Chlorotabanus mexicanus (L., 1758), and Tabanus yucatanus Townsend, 1897. C1 [Nalen, Catherine M. Zettel; Kline, Daniel L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Sutton, Bruce D.] Florida Dept Agr & Consumer Serv, Dept Plant Ind, Gainesville, FL 32607 USA. [Mueller, Guenter] Hebrew Univ Jerusalem, Kuvin Ctr Study Infect & Trop Dis, Inst Med Res Israel Canada, Dept Microbiol & Mol Genet, Jerusalem, Israel. [Cilek, James E.] Navy Entomol Ctr Excellence, Dept Testing & Evaluat, Jacksonville, FL 32212 USA. RP Nalen, CMZ (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM cnalen@wcchd.org NR 72 TC 0 Z9 0 U1 2 U2 5 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 479 EP 488 PG 10 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400014 ER PT J AU Klingeman, WE Hansen, JA Basham, JP Oliver, JB Youssef, NN Swink, W Nalepa, CA Fare, DC Moulton, JK AF Klingeman, William E. Hansen, Jason A. Basham, Joshua P. Oliver, Jason B. Youssef, Nadeer N. Swink, Whitney Nalepa, Christine A. Fare, Donna C. Moulton, J. Kevin TI Seasonal flight activity and distribution of metallic woodboring beetles (Coleoptera: Buprestidae) collected in North Carolina and Tennessee SO FLORIDA ENTOMOLOGIST LA English DT Article DE biosurveillance; Cerceris fumipennis; intrastate distribution; jewel beetles; landscape; non-native insects; nursery; pest monitoring ID CERCERIS-FUMIPENNIS HYMENOPTERA; AGRILUS CURTIS COLEOPTERA; MEXICO COLEOPTERA; UNITED-STATES; CRABRONIDAE; BORER; RECORDS; AMERICA; MANAGEMENT; CANADA AB Distribution records and seasonal flight activity information for metallic woodboring beetle (Coleoptera: Buprestidae) species have not been compiled for North Carolina and Tennessee. Institutional, research, and private collections in North Carolina and Tennessee were reviewed to provide seasonal activity data of 5 subfamilies of buprestid beetle species. Label information was checked for 15,217 specimens of 135 species collected between 1901 and 2013 (North Carolina) and between 1934 and 2013 (Tennessee). These collections provided data on adult seasonal activity and county records for 121 species (4,467 specimens) and 105 species (10,750 specimens) from North Carolina and Tennessee, respectively. Two species, Agrilus carpini Knull and A. pensus Horn, are reported as New State Records for North Carolina. The data reveal key geographic areas in both states where few to no collections have been made, highlighting opportunities to validate species distributions and locations where future collecting efforts can be matched with the occurrence of larval and adult host plant resources. Seasonal activity records will inform future biosurveillance efforts for invasive and endemic pests and facilitate predictions of buprestid species that are likely to be active within the hunting flight season of Cerceris fumipennis (Say) (Hymenoptera: Crabronidae) wasps. Activity periods of the buprestids also can focus the management of selected economic pest species to times of the year when treatment efforts, particularly through use of contact insecticides, are likely to be most effective. C1 [Klingeman, William E.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Hansen, Jason A.; Moulton, J. Kevin] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Basham, Joshua P.; Oliver, Jason B.; Youssef, Nadeer N.] Tennessee State Univ, Coll Agr Human & Nat Sci, Otis L Floyd Nursery Res Ctr, McMinnville, TN 37110 USA. [Swink, Whitney; Nalepa, Christine A.] North Carolina Dept Agr & Consumer Serv, Beneficial Insects Lab, Raleigh, NC 27699 USA. [Fare, Donna C.] USDA ARS, Otis L Floyd Nursery Res Ctr, US Natl Arboretum, McMinnville, TN 37110 USA. RP Klingeman, WE (reprint author), Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. EM wklingem@utk.edu FU University of Tennessee Institute of Agriculture, Tennessee State University College of Agriculture, Human and Natural Sciences; North Carolina Department of Agriculture and Consumer Services, USDA APHIS, and USDA NIFA; Discover Life in America [DLIA2009-07]; Forest Health Protection, USDA Forest Service [10-DG-11083137-002]; USDA-ARS [58-6404-7-213] FX We thank Christopher Brown [TTUC] for access to their entomological teaching collection, Bob Blinn [NCSU], Richard Hoebeke [UGCA], Philip Perkins [MCZ], and Adriean Mayor and Becky Nichols [GSMC] for access to collections and professional assistance. We appreciate Henry Hespenheide, Ted MacRae, Steve Paiero, Stan Wellso, and Richard Westcott for both taxonomic identifications and confirmations of species identities across the various projects. We also thank Phil Flanagan, Jason Basham, and numerous laboratory assistants during the past 12 yr for their assistance in specimen processing. Special thanks also are extended to Rich Maxey, University of Tennessee Communications and Marketing Department, for his assistance in creating the Tennessee County records map. In North Carolina, we are grateful to Rebecca Norris, Kathy Kidd, David Allgood, Alan Larkins, Phil Groves, David Pearce, Ginger Hemmings, and Sue Dial for help with Cerceris wasp surveys and biosurveillance. This project was supported, in part, by The University of Tennessee Institute of Agriculture, Tennessee State University College of Agriculture, Human and Natural Sciences, the North Carolina Department of Agriculture and Consumer Services, USDA APHIS, and USDA NIFA, and as value-added outputs resulting from collections made with Discover Life in America (#DLIA2009-07), Forest Health Protection, USDA Forest Service (#10-DG-11083137-002) and USDA-ARS (#58-6404-7-213) grants. NR 62 TC 2 Z9 2 U1 0 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 579 EP 587 PG 9 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400030 ER PT J AU Allen, ML AF Allen, Margaret Louise TI Greenhouse evaluation of neonate and adult applications of Coleomegilla maculata (Coleoptera: Coccinellidae) to control twospotted spider mite infestations SO FLORIDA ENTOMOLOGIST LA English DT Article DE lady beetle; Tetranychus urticae; impatiens; ornamental plant; generalist predator ID BIOLOGICAL-CONTROL STRATEGIES; PREDATORY INSECTS; PLANT TRICHOMES; TETRANYCHIDAE; IMPATIENS; ACARI AB The lady beetle Coleomegilla maculata DeGeer (Coleoptera: Coccinellidae) is a euryphagous predator that is known to consume the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Spider mites are frequently problematic in greenhouse operations and often cause damage to the ornamental and bedding plant Impatiens walleriana Hook f. (Ericales: Balsaminaceae). Increasing demand for alternatives to pesticides combined with resistance to registered pest control products necessitates the evaluation of viable alternatives. In this study, naive impatiens were intentionally infested with twospotted spider mites. When damage was visible on experimental plants, they were treated with lady beetles in containment cages. One treatment was adult Coleomegilla maculata and another treatment was neonate Coleomegilla maculata using recently developed methods for rearing and harvesting eggs. Levels of spider mite infestation were assessed after treatments, and images of the treated and untreated plants were collected to assess the effectiveness of Coleomegilla maculata in a greenhouse environment. Plants with Coleomegilla maculata treatments were essentially free of spider mites. There is potential to use mass-reared Coleomegilla maculata eggs as treatment for spider mites in contained crops or indoor decorative plants. C1 USDA ARS, Natl Biol Control Lab, Stoneville, MS 38776 USA. RP Allen, ML (reprint author), USDA ARS, Natl Biol Control Lab, 59 Lee Rd, Stoneville, MS 38776 USA. EM meg.allen@ars.usda.gov OI Allen, Margaret/0000-0002-4758-1290 NR 22 TC 0 Z9 0 U1 2 U2 11 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 714 EP 720 PG 7 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400047 ER PT J AU Lapointe, SL Keathley, CP Stelinski, LL Urrutia, WH Mafra-Neto, A AF Lapointe, S. L. Keathley, Craig P. Stelinski, L. L. Urrutia, W. H. Mafra-Neto, A. TI Disruption of the leafminer Phyllocnistis citrella (Lepidoptera: Gracillariidae) in citrus: effect of blend and placement height, longevity of disruption and emission profile of a new dispenser SO FLORIDA ENTOMOLOGIST LA English DT Article DE sex pheromone; (Z,Z,E)-7,11,13-hexadecatrienal; citrus leafminer; SPLAT; DCEPT CLM ID MATING DISRUPTION; SEX-PHEROMONE; GAPS; IDENTIFICATION; MECHANISM; COVERAGE; STAINTON; CANKER AB Recent efforts to disrupt mating of the leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), a global pest of citrus, have focused on the use of SPLAT(TM) (ISCA Technologies), a flowable wax emulsion intended to serve as a slow-release matrix for pheromones. Early success with this approach was overshadowed by the expense and difficulty of application, and variation in wax component chemistry that contributed to reduced longevity of pheromone emission in the field. Solid elastomer dispensers (DCEPT CLM(TM), ISCA Technologies, Inc.) loaded with a 3:1 blend of (Z,Z,E)-7,11,13-hexadecatrienal and (Z,Z)-7,11-hexadecadienal, the major components of the Phyllocnistis citrella sex pheromone, provided disruption of trap catch in commercial citrus orchards for periods exceeding 30 wk. The triene component alone worked as well as or better than the 3:1 blend. The height of dispensers placed by hand in the tree canopy had a significant effect on trap shutdown. Dispensers placed low (0.6 m) in the canopy resulted in a reduction of trap shutdown in the upper third (>3 m) of the canopy suggesting that the net movement of pheromone molecules was downward during the period of active moth flight. Although moth flight appeared equivalent among the heights tested, placement of dispensers higher in the canopy appears more effective given this downward movement of pheromone plumes. These studies suggest that season-long trap catch disruption can be attained in citrus with a single application of a hand-applied dispenser. C1 [Lapointe, S. L.; Keathley, Craig P.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Stelinski, L. L.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. [Urrutia, W. H.; Mafra-Neto, A.] ISCA Technol Inc, Riverside, CA 92507 USA. RP Lapointe, SL (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM stephen.lapointe@ars.usda.gov FU Citrus Research and Development Foundation FX Larry Markle, Rylan Mermer, Josh MacNaught, Jiemin Liu, and Dr. Paul Robbins (USDA, ARS, Ft. Pierce, Florida), Wendy Meyer, and Ian Jackson (University of Florida, Lake Alfred, Florida) provided technical assistance in the field. Rocco Alessandro (USDA, ARS, Ft. Pierce, Florida) provided GC-MS analysis of DCEPT CLM dispensers. We are particularly grateful to David Kemeny (TRB Groves, Punta Gorda, Florida), Scott Lambeth (Golden River Fruit Company, Vero Beach, Florida), and Tom Stopyra (The Packers of Indian River, Ft. Pierce, Florida), for arranging access to those properties. Significant funding was provided by a grant from the Citrus Research and Development Foundation. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the United States Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. NR 15 TC 1 Z9 1 U1 5 U2 13 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 742 EP 748 PG 7 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400051 ER PT J AU Diaz, R Dickey, AM Shatters, RG Manrique, V Vitorino, MD Overholt, WA AF Diaz, Rodrigo Dickey, Aaron M. Shatters, Robert G., Jr. Manrique, Veronica Vitorino, Marcelo D. Overholt, William A. TI New species diversity revealed from molecular and morphological characterization of gall-inducing Calophya spp. (Hemiptera: Calophyidae) from Brazilian peppertree SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE natural enemy; Brazilian peppertree; leaflet gall inducer; phylogenetic analysis; weed biological control ID PLANT-LICE HEMIPTERA; SCHINUS-TEREBINTHIFOLIUS; FLORIDA; ANACARDIACEAE; LATIFORCEPS; HISTORY; DNA AB Four populations of Calophya spp. (Hemiptera: Calophyidae) collected in Brazil were characterized using molecular and morphological methods. Examination of adults revealed the presence of 2 morphotypes, which were identified as C. latiforceps and C. terebinthifolii. However, morphological examination of 5th instar nymphs detected differences within C. latiforceps, with a population from Ubu, Espirito Santo, being distinct from the other 2 populations. Molecular characterization of the mitochondrial CO1 gene supported the presence of a new species of Calophya. C1 [Diaz, Rodrigo; Manrique, Veronica; Overholt, William A.] Univ Florida, Indian River Res & Educ Ctr, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. [Dickey, Aaron M.; Shatters, Robert G., Jr.] USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Vitorino, Marcelo D.] Fundacao Univ Reg Blumenau, BR-89012900 Blumenau, SC, Brazil. RP Overholt, WA (reprint author), Univ Florida, Indian River Res & Educ Ctr, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. EM billover@ufl.edu NR 22 TC 1 Z9 1 U1 0 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 776 EP 779 PG 4 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400057 ER PT J AU Carrillo, D Narvaez, T Cosse, AA Stouthamer, R Cooperband, M AF Carrillo, Daniel Narvaez, Tereza Cosse, Allard A. Stouthamer, Richard Cooperband, Miriam TI Attraction of Euwallacea nr. fornicatus (Coleoptera: Curculionidae: Scolytinae) to lures containing quercivorol SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE avocado; ambrosia beetle; attractant; quercivorol ID AMBROSIA BEETLE; AGGREGATION PHEROMONE; AVOCADO; (1S,4R)-P-MENTH-2-EN-1-OL; PLATYPODIDAE AB Euwallacea nr. fornicatus (Eichhoff) (Coleoptera: Curculionidae: Scolytinae) is an exotic ambrosia beetle that vectors fungal Fusarium spp. to avocados. Two field trials testing potential attractants to trap Euwallacea spp. were conducted in south Florida. Quercivorol + Ultra High Release Ethanol (URH) was the most powerful attractant for Euwallacea nr. fornicatus in the 2 field trials. In Florida, the populations of Euwallacea nr. fornicatus are small. The attractiveness of quercivorol lures to other Euwallacea spp. should be tested in conditions where population levels are greater as in California and Israel. C1 [Carrillo, Daniel; Narvaez, Tereza] Univ Florida, Trop Res & Educ Ctr, Homestead, FL 33031 USA. [Cosse, Allard A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Stouthamer, Richard] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Cooperband, Miriam] USDA APHIS, Otis Lab, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. RP Carrillo, D (reprint author), Univ Florida, Trop Res & Educ Ctr, 18905 SW 280 ST, Homestead, FL 33031 USA. EM dancar@ufl.edu NR 7 TC 2 Z9 3 U1 5 U2 265 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 780 EP 782 PG 3 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400058 ER PT J AU Varone, L Logarzo, G Martinez, JJ Navarro, F Carpenter, JE Hight, SD AF Varone, Laura Logarzo, Guillermo Jose Martinez, Juan Navarro, Fernando Carpenter, James E. Hight, Stephen D. TI Field host range of Apanteles opuntiarum (Hymenoptera: Braconidae) in Argentina, a potential biocontrol agent of Cactoblastis cactorum (Lepidoptera: Pyralidae) in North America SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE parasitoid; host range; cactus moth; Apanteles alexanderi; Apanteles opuntiarum; biological control ID BIOLOGICAL-CONTROL AB Field exploration in Argentina for cactophagous lepidopteran hosts parasitized by the recently described braconid parasitoid Apanteles opuntiarum Martinez & Berta (Hymenoptera: Braconidae) revealed a host range restricted to Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae) and C. doddi. Field collections of cactophagous larvae parasitized by the congeneric and sympatric parasitoid A. alexanderi included the host species C. bucyrus, Sigelgaita nr. chilensis, Tucumania sp., Tucumania tapiacola and Salambona sp. If a narrow host range for A. opuntiarum is confirmed in quarantine with North American cactus-feeding species, then this parasitoid could be released as a biological control agent for C. cactorum with little or no risk to non-target species. C1 [Varone, Laura; Logarzo, Guillermo] FuEDEI, Fdn Estudio Especies Invasivas, RA-1686 Buenos Aires, DF, Argentina. [Jose Martinez, Juan] Consejo Nacl Invest Cient & Tecn, Div Entomol, Museo Argentino Ciencias Nat Bernardino Rivadavia, RA-1033 Buenos Aires, DF, Argentina. [Navarro, Fernando] Inst Super Entomol Dr Abraham Willink, RA-4000 San Miguel De Tucuman, Argentina. [Carpenter, James E.] USDA ARS CPMRU, Tifton, GA 31794 USA. [Hight, Stephen D.] USDA ARS CMAVE, Ctr Biol Control, FAMU, Tallahassee, FL 32308 USA. RP Varone, L (reprint author), FuEDEI, Fdn Estudio Especies Invasivas, RA-1686 Buenos Aires, DF, Argentina. EM lauvarone@fuedei.org NR 15 TC 0 Z9 1 U1 1 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 803 EP 806 PG 4 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400065 ER PT J AU Lapointe, SL AF Lapointe, Stephen L. TI A tribute to Dr. Anthony C. Bellotti and his contributions to cassava entomology SO FLORIDA ENTOMOLOGIST LA English DT Biographical-Item DE classical biological control; cassava mealybug; cassava green mite; Peace Corps; CGIAR AB Anthony (Tony) Bellotti's career as a humanitarian and entomologist followed a trajectory that took him to El Salvador with the Peace Corps (PC) in 1962, New Mexico State for a Masters, Paraguay (again with the Peace Corps), Cornell University for a Ph.D., and Colombia where he worked for the Centro Internacional de Agricultura Tropical (CIAT) from 1974 until his passing in March 2013 in Naples, Florida. Tony became a passionate advocate of cassava, and one of the world's pre-eminent authorities on the entomology of that orphan crop that sustains millions of the world's poorest populations. Tony played a crucial role in one of the most often cited and successful examples of classical biological control, the introduction of a parasitoid wasp from Paraguay to control the cassava mealybug throughout a broad area of Africa known as the Cassava Belt. His career spanned a period of time that might be referred to as a golden age of commodity programs at the international "CG Centers" organized under the Consultative Group on International Agricultural Research (CGIAR), which followed in the footsteps of Norman Borlaug and Robert McNamara, the unlikely alliance that led to the Green Revolution. Among Tony's lesser accomplishments was his recruitment of the author to CIAT in 1986, where I worked on pests of tropical forages and then on cassava integrated pest management in northeastern Brazil and West Africa. Tony was a mentor and a friend whose career offers a chance to look at a unique life and his contributions to international agriculture. C1 USDA ARS, United States Hort Res Lab, Ft Pierce, FL 34945 USA. RP Lapointe, SL (reprint author), USDA ARS, United States Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM stephen.lapointe@ars.usda.gov NR 23 TC 0 Z9 0 U1 7 U2 11 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2015 VL 98 IS 2 BP 810 EP 814 PG 5 WC Entomology SC Entomology GA CK7XY UT WOS:000356451400067 ER PT J AU Mason, CJ Klepzig, KD Kopper, BJ Kersten, P Illman, BL Raffa, KF AF Mason, Charles J. Klepzig, Kier D. Kopper, Brian J. Kersten, Philip J. Illman, Barbara L. Raffa, Kenneth F. TI Contrasting Patterns of Diterpene Acid Induction by Red Pine and White Spruce to Simulated Bark Beetle Attack, and Interspecific Differences in Sensitivity Among Fungal Associates SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Plant defense; Terpenoids; Ophiostoma; Leptographium; Ips; Dendroctonus ID DENDROCTONUS-RUFIPENNIS COLEOPTERA; NORWAY SPRUCE; CONIFER DEFENSE; PICEA-ABIES; IPS-PINI; CERATOCYSTIS-POLONICA; CHEMICAL DEFENSES; LODGEPOLE PINE; SITKA SPRUCE; RESIN ACIDS AB Conifers possess a suite of physiochemical defenses that protect their subcortical tissues from bark beetle - fungal complexes. These defenses include rapid induction of terpenoids and phenolics at the site of attack. Studies of the distribution, induction, and bioactivity of conifer terpenoids have focused heavily on monoterpenes. We assessed induction of diterpene acids in white spruce (Picea glauca) and red pine (Pinus resinosa) to fungal associates of two bark beetles, and the responses of four spruce beetle (Dendroctonus rufipennis)-associated fungi to three diterpene acids. Constitutive phloem contents differed between species, in that red pine had extremely low concentrations of diterpene acids, whereas white spruce had substantial constitutive levels. Induction differed quantitatively. Both red pine and white spruce exhibited marked increases, but red pine underwent greater increases and achieved higher concentrations than white spruce. Induction also differed qualitatively in that red pine showed lower diversity and fewer compositional changes during induction than white spruce. In red pine,fungal inoculation accompanying wounding elicited greater increases than wounding alone, but in white spruce total concentrations were higher following wounding alone. Spruce beetle fungal symbiont growth varied among species and compounds. Some diterpenes elicited both stimulatory and inhibitory effects on fungi, depending on concentration. All four fungi exhibited higher tolerances compared to those associated with pine bark beetles in previous studies. Variation in tolerances to, and potentially metabolism of, diterpene acids by symbionts may reflect differences in constitutive levels between spruce and pine, and partially explain differences in concentrations achieved during induction. C1 [Mason, Charles J.; Kopper, Brian J.; Raffa, Kenneth F.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Mason, Charles J.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Klepzig, Kier D.] ARS, Southern Res Stn, USDA, Asheville, NC 28804 USA. [Kopper, Brian J.] USDA APHIS PPQ, Raleigh, NC 27606 USA. [Kersten, Philip J.; Illman, Barbara L.] ARS, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Raffa, Kenneth F.] Univ Wisconsin, Russell Labs 345, Madison, WI 53706 USA. RP Raffa, KF (reprint author), Univ Wisconsin, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM raffa@entomology.wisc.edu FU McEntire Stennis [WIS01430]; University of Wisconsin-Madison College of Agricultural and Life Sciences FX This work was funded by McEntire Stennis WIS01430 and the University of Wisconsin-Madison College of Agricultural and Life Sciences. Critical reviews by two anonymous reviewers helped improve this paper. NR 60 TC 2 Z9 2 U1 3 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2015 VL 41 IS 6 BP 524 EP 532 DI 10.1007/s10886-015-0588-4 PG 9 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CK2KT UT WOS:000356040000003 PM 26003180 ER PT J AU Downey, D Chun, S Follett, P AF Downey, Danielle Chun, Stacey Follett, Peter TI Radiobiology of Small Hive Beetle (Coleoptera: Nitidulidae) and Prospects for Management Using Sterile Insect Releases SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Aethina tumida; honey bee; Apis mellifera; x-ray radiation; sterile insect technique ID AETHINA-TUMIDA COLEOPTERA; BIOLOGY; TEPHRITIDAE; LONGEVITY; COLONIES; DIPTERA; FLY AB Small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is considered a serious threat to beekeeping in the Western Hemisphere, Australia, and Europe mainly due to larval feeding on honey, pollen, and brood of the European honeybee, Apis mellifera L. Control methods are limited for this pest. Studies were conducted to provide information on the radiobiology of small hive beetle and determine the potential for sterile insect releases as a control strategy. Adult males and females were equally sensitive to a radiation dose of 80 Gy and died within 5-7 d after treatment. In reciprocal crossing studies, irradiation of females only lowered reproduction to a greater extent than irradiation of males only. For matings between unirradiated males and irradiated females, mean reproduction was reduced by > 99% at 45 and 60 Gy compared with controls, and no larvae were produced at 75 Gy. Irradiation of prereproductive adults of both sexes at 45 Gy under low oxygen (1-4%) caused a high level of sterility (> 99%) while maintaining moderate survivorship for several weeks, and should suffice for sterile insect releases. Sterile insect technique holds potential for suppressing small hive beetle populations in newly invaded areas and limiting its spread. C1 [Downey, Danielle; Chun, Stacey] Hawaii Dept Agr, Hilo, HI 96720 USA. [Follett, Peter] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Follett, P (reprint author), USDA ARS, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM peter.follett@ars.usda.gov NR 22 TC 2 Z9 2 U1 1 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 868 EP 872 DI 10.1093/jee/tov068 PG 5 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000004 PM 26470205 ER PT J AU Strange, JP AF Strange, James P. TI Bombus huntii, Bombus impatiens, and Bombus vosnesenskii (Hymenoptera: Apidae) Pollinate Greenhouse-Grown Tomatoes in Western North America SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE bumble bee; pollination; protected cultivation; tomato ID TERRESTRIS L. HYMENOPTERA; BUMBLE BEES BOMBUS; COMMERCIALIZATION; POPULATIONS AB Bumble bees (Bombus) are the primary pollinators of tomatoes grown in greenhouses and can significantly increase fruit weight compared with tomatoes that receive no supplemental pollination. More than a million colonies are sold worldwide annually to meet pollination needs. Due to mounting concerns over the transportation of bumble bees outside of their native ranges, several species native to western North American are currently being investigated as potential commercial pollinators. Here, two western, Bombus huntii Greene and Bombus vosnesenskii Radoszkowski, and one eastern species, Bombus impatiens Cresson, are compared for their efficacy as pollinators of greenhouse-grown tomatoes. In two experiments, colonies were placed in greenhouses and compared with control plants that received no supplemental pollination. In the first experiment, seed set was significantly increased with B. huntii pollination in one variety of cherry tomatoes. In the second experiment comparing all three bumble bee species, fruit weight was an average of 25.2 g heavier per fruit pollinated by bees versus the control, and the number of days to harvest was 2.9 d shorter for bee-pollinated fruit. In some rounds of pollination, differences were found among bumble bee species, but these were inconsistent across replicates and not statistically significant overall. Additionally, fruit weight was shown to be highly correlated to fruit diameter and seed set in all tests and, thus, is shown to be a reliable metric for assessing pollination in future studies. These results suggest that commercialization of western bumble bees is a viable alternative to the current practices of moving of nonnative bees into western North America to pollinate tomatoes. C1 Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. RP Strange, JP (reprint author), Utah State Univ, USDA ARS, Pollinating Insect Res Unit, 255 BNR, Logan, UT 84322 USA. EM James.Strange@ars.usda.gov NR 24 TC 1 Z9 1 U1 6 U2 16 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 873 EP 879 DI 10.1093/jee/tov078 PG 7 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000005 PM 26470206 ER PT J AU Meikle, WG Holst, N Cook, SC Patt, JM AF Meikle, William G. Holst, Niels Cook, Steven C. Patt, Joseph M. TI Variability in Small Hive Beetle (Coleoptera: Nitidulidae) Reproduction in Laboratory and Field Experiments SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE A. tumida; honey bee; population dynamics; temperature effect; field trial ID AETHINA-TUMIDA COLEOPTERA; MELLIFERA-CAPENSIS ESCH.; EUROPEAN HONEY-BEES; APIS-MELLIFERA; COLONIES; REMOVAL; SUCCESS; MURRAY; LARVAE; OVIPOSITION AB Experiments were conducted to examine how several key factors affect population growth of the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae). Laboratory experiments were conducted to examine effects of food quantity and temperature on reproduction of cohorts of young A. tumida adults (1:1 sex ratio) housed in experimental arenas. Daily numbers and total mass of larvae exiting arenas were highly variable within treatment. Either one or two cohorts of larvae were observed exiting the arenas. Food quantity, either 10 g or 20 g, did not significantly affect the number of larvae exiting arenas at 32A degrees C, but did at 28A degrees C; arenas provided 20 g food produced significantly more larvae than arenas provided 10 g. Temperature did not affect the total mass of larvae provided 10 g food, but did affect larval mass provided 20 g; beetles kept at 28A degrees C produced more larval mass than at 32A degrees C. Field experiments were conducted to examine A. tumida reproductive success in full strength bee colonies. Beetles were introduced into hives as egg-infested frames and as adults, and some bee colonies were artificially weakened through removal of sealed brood. Efforts were unsuccessful; no larvae were observed exiting from, or during the inspection of, any hives. Possible reasons for these results are discussed. The variability observed in A. tumida reproduction even in controlled laboratory conditions and the difficulty in causing beetle infestations in field experiments involving full colonies suggest that accurately forecasting the A. tumida severity in such colonies will be difficult. C1 [Meikle, William G.] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Holst, Niels] Aarhus Univ, Dept Agroecol, DK-8000 Aarhus C, Denmark. [Cook, Steven C.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Patt, Joseph M.] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. RP Meikle, WG (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. EM william.meikle@ars.usda.gov NR 31 TC 1 Z9 1 U1 1 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 887 EP 893 DI 10.1093/jee/tov101 PG 7 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000007 PM 26470208 ER PT J AU Thinakaran, J Pierson, EA Longnecker, M Tamborindeguy, C Munyaneza, JE Rush, CM Henne, DC AF Thinakaran, Jenita Pierson, E. A. Longnecker, M. Tamborindeguy, C. Munyaneza, J. E. Rush, C. M. Henne, D. C. TI Settling and Ovipositional Behavior of Bactericera cockerelli (Hemiptera: Triozidae) on Solanaceous Hosts Under Field and Laboratory Conditions SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Potato psyllid; Bactericera cockerelli; host preference; settling behavior ID PLANT SELECTION; PHYTOPHAGOUS INSECTS; NORTH-AMERICA; POTATO CROPS; LIBERIBACTER; DISEASE; SULC; TRANSMISSION; POPULATIONS; MANAGEMENT AB Potato psyllid, Bactericera cockerelli (Sulc), is a seasonal insect pest in the Lower Rio Grande Valley of Texas, where it transmits the bacterial pathogen "Candidatus Liberibacter solanacearum" that causes zebra chip disease of potato. Studies were conducted to evaluate host preference of B. cockerelli adults for different plant species, and plant size and density. Settling and oviposition behavior of B. cockerelli was studied on its wild and cultivated solanaceous hosts, including potato, tomato, pepper, eggplant, and silverleaf nightshade, under both field and laboratory conditions. Naturally occurring B. cockerelli were used to evaluate host preference under open field conditions throughout the growing season. Settling and oviposition preference studies in the laboratory were conducted as cage-release experiments using pairs of plants, and observations were recorded over a 72-h period. Results of field trials indicated that naturally occurring B. cockerelli preferred potato and tomato equally for settling and oviposition, but settled on pepper, eggplant, and silverleaf nightshade only in the absence of potato and tomato. Under laboratory conditions, B. cockerelli adults preferred larger host plants, regardless of the species tested. Results also showed that movement of B. cockerelli was minimal after initial landing and settling behavior was influenced by host plant density. Lone plants attracted the most psyllids and can be used as sentinel plants to monitor B. cockerelli activity. Information from both field and laboratory studies demonstrated that not only host plant species determined host selection behavior of B. cockerelli adults, but also plant size and density. C1 [Thinakaran, Jenita; Henne, D. C.] Texas A&M AgriLife Res, Weslaco, TX 78596 USA. [Thinakaran, Jenita; Munyaneza, J. E.] USDA ARS, Wapato, WA 98951 USA. [Pierson, E. A.] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Longnecker, M.] Texas A&M Univ, Dept Stat, College Stn, TX 77843 USA. [Tamborindeguy, C.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Rush, C. M.] Texas A& M AgriLife Res, Amarillo, TX 79106 USA. RP Thinakaran, J (reprint author), Texas A&M AgriLife Res, Weslaco, TX 78596 USA. EM Jenita.Thinakaran@ars.usda.gov FU United States Department of Agriculture - Risk Avoidance and Mitigation Program [2009-51101-05892]; United States Department of Agriculture - Specialty Crops Research Initiative [2009-51181-20178] FX We thank T. Salazar, C. Bonczyk, A. Pena, and E. Huerta for technical assistance and Dr. J. Creighton Miller for his valuable contribution in reviewing earlier versions of this manuscript. Funding provided by United States Department of Agriculture - Risk Avoidance and Mitigation Program grant (2009-51101-05892) and United States Department of Agriculture - Specialty Crops Research Initiative grant (2009-51181-20178) is gratefully acknowledged. NR 42 TC 0 Z9 0 U1 4 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 904 EP 916 DI 10.1093/jee/tov058 PG 13 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000009 PM 26470210 ER PT J AU Watt, TJ Duan, JJ Tallamy, DW Hough-Goldstein, J AF Watt, Timothy J. Duan, Jian J. Tallamy, Douglas W. Hough-Goldstein, Judith TI Effect of Parasitoid: Host Ratio and Group Size on Fitness of Spathius galinae (Hymenoptera: Braconidae): Implications for Mass-Rearing SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biological control; invasive pest; laboratory rearing; Agrilus planipennis; wood borer ID BORER COLEOPTERA BUPRESTIDAE; LOCAL MATE COMPETITION; RUSSIAN FAR-EAST; SEX-RATIO; BIOLOGICAL-CONTROL; EULOPHIDAE; BEHAVIOR; EGG; PARAMETERS; MICHIGAN AB Producing insect natural enemies in laboratories or insectaries for biological pest control is often expensive, and developing cost-effective rearing techniques is a goal of many biological control programs. Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a newly described ectoparasitoid of emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is currently being evaluated for environmental introduction in the United States to provide biological control of this invasive pest. To improve mass-rearing outcomes for S. galinae, we investigated the effects of parasitoid: host ratio and parasitoid and host group size (density) on parasitoid fitness. Our results showed that when 1 emerald ash borer larva was exposed to 1, 2, 4, or 8 female parasitoids, parasitism rate was positively associated with increasing parasitoid: host ratio, while brood size, sex ratio, and fitness estimates of progeny were not affected. When a constant 1:1 parasitoid: host ratio was used, but group size varied from 1 female parasitoid and 1 host, 5 parasitoids and 5 hosts, 10 of each, and 20 of each in same size rearing cages, parasitism rates were highest when at least 5 females were exposed to 5 host larvae. Moreover, the number of progeny produced per female parasitoid was greatest when group size was 10 parasitoids and 10 hosts. These findings demonstrate that S. galinae may be reared most efficiently in moderately high-density groups (10 parasitoids and hosts) and with a 1:1 parasitoid: host ratio. C1 [Watt, Timothy J.; Tallamy, Douglas W.; Hough-Goldstein, Judith] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA. [Watt, Timothy J.; Duan, Jian J.] USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. RP Watt, TJ (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA. EM tjwatt@udel.edu FU United States Department of Agriculture-Animal and Plant Health Inspection Service grant [58-1926-2-282] FX We thank Kristi Larson, Jackie Hoban, Sue Barth (University of Delaware), Jonathan Schmude, and Cherie Keenan (United States Department of Agriculture-Agricultural Research Service) for assistance maintaining insect cultures. We are grateful to Dick Bean, Kim Rice, and Steve Bell (Maryland Department of Agriculture) for providing emerald ash borer adults that were needed for laboratory egg production. We thank Doug Luster (United States Department of Agriculture-Agricultural Research Service) for reviewing the manuscript. This research was supported through funding from United States Department of Agriculture-Animal and Plant Health Inspection Service grant 58-1926-2-282. NR 32 TC 2 Z9 2 U1 1 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 951 EP 956 DI 10.1093/jee/tov062 PG 6 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000014 PM 26470215 ER PT J AU Zilkowski, BW Cosse, AA AF Zilkowski, Bruce W. Cosse, Allard A. TI A Culture Method for Darkling Beetles, Blapstinus spp. (Coleoptera: Tenebrionidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Blapstinus; cucurbit; mass-rearing; insect culture; sex determination ID ALPHITOBIUS-DIAPERINUS; AGGREGATION PHEROMONE; LESSER MEALWORM AB Darkling beetles, Blapstinus spp., have become a serious pest of Cucurbitaceae crops, especially in California. A culture method was sought to provide large numbers (>500) of adult beetles of known age and sex that could be used for laboratory testing when needed. A method previously developed for Alphitobius diaperinus (Panzer) using a diet of ground chick feed, with apple slices as a moisture source, was modified for use with Blapstinus spp. and then compared with the same method substituting apple slices with zucchini as the moisture source. Rearing boxes set up with apple slices produced significantly more pupae and adults than boxes containing zucchini slices. However, using either zucchini or apples as a moisture source yielded over the target of 500 adults per rearing box. A previous method designed to sex A. diaperinus based on the presence (female) or absence (male) of second valvifers in the pupal stage also proved to be effective for sexing the Blapstinus spp. C1 [Zilkowski, Bruce W.; Cosse, Allard A.] ARS, USDA, NCAUR, Peoria, IL 61604 USA. RP Zilkowski, BW (reprint author), ARS, USDA, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM Bruce.Zilkowski@ars.usda.gov NR 7 TC 0 Z9 0 U1 4 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1010 EP 1013 DI 10.1093/jee/tov040 PG 4 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000022 PM 26470223 ER PT J AU Sisterson, MS Wallis, CM Stenger, DC AF Sisterson, Mark S. Wallis, Christopher M. Stenger, Drake C. TI Effects of Glassy-Winged Sharpshooter (Hemiptera: Cicadellidae) Feeding, Size, and Lipid Content on Egg Maturation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Xylella fastidiosa; Pierce's disease; vector ecology; Cicadellidae; fat body ID HOMALODISCA-VITRIPENNIS; TRADE-OFFS; BODY-SIZE; HOST; INSECTS; LOAD; OVIPOSITION; BUTTERFLIES; METABOLISM; COAGULATA AB The glassy-winged sharpshooter (Homalodisca vitripennis) is synovigenic and must feed as an adult to produce eggs. Egg maturation rates depend on the host plant species provided to the adult female for feeding and are variable for females provided with the same host plant species. Here, the contribution of female size and lipid content to variation in egg maturation rates among females held on the same host plant species was assessed. To assess effects of female size and lipid content on egg maturation, feeding assays followed by measurements of egg load, female size, and lipid content were conducted. To accomplish this, females were field collected and held on cowpea until producing approximately 0, 12, 25, or 50 ml of excreta. After reaching prescribed excreta thresholds, females were dissected to determine egg load, hind tibia length, and head capsule width. Mature eggs were removed from the abdomen and dry weight of eggs and bodies (head, thorax, and abdomen) were obtained. Lipid content of eggs and bodies were determined using a quantitative colorimetric assay. Rates of body weight gain and body lipid gain were rapid with low levels of feeding (12 ml of excreta) but decelerated with additional feeding (>12 ml of excreta). In contrast, low levels of feeding (12 ml of excreta) resulted in little egg production, with rates of egg production accelerating with additional feeding (>12 ml of excreta). Accordingly, egg production was preceded by an increase in body dry weight and body lipid content. In agreement, probability that a female carried eggs increased with body lipid content in the 0-, 12-, and 25-ml feeding treatments. Across treatments, larger females carried more eggs than smaller females. Collectively, results suggest that variation in glassy-winged sharpshooter egg maturation rates partially may be explained by availability of lipid reserves at the start of a feeding bout and female size. C1 [Sisterson, Mark S.; Wallis, Christopher M.; Stenger, Drake C.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Sisterson, MS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM mark.sisterson@ars.usda.gov FU USDA-ARS [2034-22000-010-00D] FX We thank Rodrigo Krugner and David Bellamy for discussion. We also thank Sean Uchima and Donal Dwyer for technical assistance. Funding for this project was from USDA-ARS appropriated project 2034-22000-010-00D. NR 46 TC 1 Z9 1 U1 4 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1014 EP 1024 DI 10.1093/jee/tov049 PG 11 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000023 PM 26470224 ER PT J AU Hardke, JT Jackson, RE Leonard, BR Temple, JH AF Hardke, Jarrod T. Jackson, Ryan E. Leonard, B. Rogers Temple, Joshua H. TI Fall Armyworm (Lepidoptera: Noctuidae) Development, Survivorship, and Damage on Cotton Plants Expressing Insecticidal Plant-Incorporated Protectants SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Spodoptera frugiperda; Gossypium hirsutum; larval-pupal survivorship; Cry1Ac; Cry2Ab ID BACILLUS-THURINGIENSIS; BOLLWORM LEPIDOPTERA; TRANSGENIC COTTON; LARVAL SURVIVAL; BEET ARMYWORM; PROTEINS; ENDOTOXIN; EFFICACY; CRY1AC; FIELD AB Cotton, Gossypium hirsutum (L.), plants expressing insecticidal crystal (Cry) proteins of Bacillus thuringiensis (Bt) Berliner are planted on significant acreage across the southern region of the United States. Fall armyworm, Spodoptera frugiperda (J. E. Smith), can be a significant cotton pest in some years, but this species has not been a primary target of Bt cotton technologies. The objective of this study was to quantify fall armyworm larval survivorship and fruiting form injury on transgenic cotton lines expressing Cry1Ac (Bollgard), Cry1Ac + Cry2Ab (Bollgard II), and Cry1Ac + Cry1F (WideStrike) Bt proteins. Larval survivorship and fruiting form damage of fall armyworm on Bollgard, Bollgard II, WideStrike, and non-Bt (control) cotton lines were evaluated in no-choice field studies. Fall armyworm (third instars) were placed on flower buds (squares), white flowers, and bolls, enclosed within a nylon mesh exclusion cage, and evaluated at selected intervals after infestation. Exposure of fall armyworm larvae to Bollgard cotton lines generally resulted in no significant effects on survivorship compared with larvae exposed to the non-Bt cotton line. Survivorship and plant injury by fall armyworm on Bollgard II cotton lines was variable compared with that on non-Bt cotton lines, and significant differences between treatments were inconsistent. Fall armyworm had significantly lower survivorship and caused less plant injury on WideStrike cotton lines than on non-Bt cotton lines across all plant structures. Development and survivorship of fall armyworm larvae on these cotton lines also were evaluated in no-choice laboratory assays by offering the previously described fruiting forms to third instars. Bollgard II and WideStrike cotton lines significantly reduced fall armyworm development and survivorship compared with those larvae offered non-Bt tissue. These results suggest that differences exist among selected Bt cotton technologies in their performance against fall armyworm. C1 [Hardke, Jarrod T.; Temple, Joshua H.] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [Jackson, Ryan E.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. [Leonard, B. Rogers] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. EM jhardke@uaex.edu FU Louisiana Cotton Producers and Cotton Incorporated; Monsanto; Dow AgroSciences FX We thank the student interns with the LSU Department of Entomology and at the LSU AgCenter's Macon Ridge Research Station for their assistance in maintaining field trials and for fall armyworm colony maintenance. We also express appreciation to Trey Price for providing critical reviews of this manuscript. This article is published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript number 2015-279-21017. This project represents work supported by the Louisiana Cotton Producers and Cotton Incorporated, and partial funding from Monsanto and Dow AgroSciences. NR 31 TC 0 Z9 0 U1 6 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1086 EP 1093 DI 10.1093/jee/tov092 PG 8 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000032 PM 26470233 ER PT J AU Dominiak, BC Campbell, AJ Jang, EB Ramsey, A Fanson, BG AF Dominiak, Bernard C. Campbell, Angus J. Jang, Eric B. Ramsey, Amanda Fanson, Benjamin G. TI Field Evaluation of Melolure, a Formate Analogue of Cuelure, and Reassessment of Fruit Fly Species Trapped in Sydney, New South Wales, Australia SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Queensland fruit fly; cuelure; melolure; Bactrocera; Dacus ID RASPBERRY KETONE FORMATE; DIPTERA TEPHRITIDAE; SURVEILLANCE; ATTRACTANT; MANAGEMENT AB In Australia, tephritids are usually attracted to either cuelure or methyl eugenol. Methyl eugenol is a very effective lure, but cuelure is less effective likely due to low volatility. A new formate analogue of cuelure, melolure, has increased volatility, resulting in improved efficacy with the melon fruit fly, Bactrocera cucurbitae Coquillett. We tested the efficacy of melolure with fruit fly species in Sydney as part of the National Exotic Fruit Fly Monitoring programme. This monitoring programme has 71 trap sites across Sydney, with each trap site comprising separate Lynfield traps containing either cuelure, methyl eugenol, or capilure lure. In 2008, an additional Lynfield trap with melolure plugs was added to seven sites. In 2009 and 2010, an additional Lynfield trap with melolure wicks was added to 11 trap sites and traps were monitored fortnightly for 2 yr. Capture rates for melolure traps were similar to cuelure traps for Dacus absonifacies (May) and Dacus aequalis (Coquillet), but melolure traps consistently caught fewer Bactrocera tryoni (Froggatt) than cuelure traps. However, trap sites with both a cuelure and melolure traps had increased capture rates for D. absonifacies and D. aequalis, and a marginally significant increase for B. tryoni. Melolure plugs were less effective than melolure wicks, but this effect may be related to lure concentration. The broader Bactrocera group species were attracted more to cuelure than melolure while the Dacus group species were attracted more to melolure than cuelure. There is no benefit in switching from cuelure to melolure to monitor B. tryoni, the most important fruit fly pest in Australia. C1 [Dominiak, Bernard C.] NSW Dept Primary Ind, Orange, NSW 2800, Australia. [Campbell, Angus J.] NSW Dept Primary Ind, Bathurst, NSW 2795, Australia. [Jang, Eric B.] USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Ramsey, Amanda] Scentry Biol Inc, Res & Dev, Billings, MT 59102 USA. [Fanson, Benjamin G.] Deakin Univ, Ctr Integrat Ecol, Sch Life & Environm Sci, Geelong, Vic 3216, Australia. RP Dominiak, BC (reprint author), NSW Dept Primary Ind, Locked Bag 21, Orange, NSW 2800, Australia. EM bernie.dominiak@dpi.nsw.gov.au FU Department of Agriculture, Fisheries and Forestry FX The Sydney National Exotic Fruit Fly Monitoring programme is largely funded by the Department of Agriculture, Fisheries and Forestry. The assistance of trap inspectors is gratefully acknowledged. The identification staff of Orange Agricultural Institute are also gratefully acknowledged. Dr Matt Siderhurst and Dr Louise Rossiter provided useful comments on an earlier version of this manuscript. NR 17 TC 3 Z9 4 U1 2 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1176 EP 1181 DI 10.1093/jee/tov048 PG 6 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000042 PM 26470243 ER PT J AU Hall, DG Rohrig, E AF Hall, David G. Rohrig, Eric TI Bionomics of Asian Citrus Psyllid (Hemiptera: Liviidae) Associated with Orange Jasmine Hedges in Southeast Central Florida, with Special Reference to Biological Control by Tamarixia radiata SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE greening disease; huanglongbing; Murraya; Diaphorina citri ID CANDIDATUS LIBERIBACTER ASIATICUS; DIAPHORINA-CITRI; MURRAYA-PANICULATA; HYMENOPTERA EULOPHIDAE; SEASONAL ABUNDANCE; HOMOPTERA; DISEASE AB The Asian citrus psyllid, Diaphorina citri Kuwayama, is an important pest in Florida because it transmits bacteria responsible for citrus huanglongbing disease. In addition to infesting citrus, orange jasmine (Murraya exotica L.) is one of Asian citrus psyllid's preferred host plants and is widely grown as an ornamental hedge. We report on Asian citrus psyllid bionomics over three years at five urban plantings of orange jasmine and on biological control of Asian citrus psyllid by a parasitoid Tamarixia radiata (Waterston). T. radiata had been released in Florida shortly after Asian citrus psyllid was first found, and the parasitoid was known to be established at each planting. Additionally, three new T. radiata haplotypes were released every 3 wk at three plantings during the first study year (one haplotype per planting, over all releases an average of 17 parasitoids per linear meter of hedge); all three haplotypes were released at a fourth planting beginning midway through the study (over all releases, an average combined total of 202 parasitoids per linear meter of hedge). Asian citrus psyllid populations were present year-round at each planting, often at large levels. Such plantings may pose risk to commercial citrus as Asian citrus psyllid reservoirs. Releases of the new haplotypes did not cause any measurable reduction in Asian citrus psyllid population levels during the study, and ironically percentage parasitism was generally highest at a planting where no releases were made. Higher release rates might have been more effective. The probability is discussed that repetitive pruning of orange jasmine reduced the full potential of T. radiata against Asian citrus psyllid in this study. C1 [Hall, David G.] USDA ARS, Ft Pierce, FL 34945 USA. [Rohrig, Eric] FDACS DPI, Gainesville, FL 32614 USA. RP Hall, DG (reprint author), USDA ARS, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM david.hall@ars.usda.gov NR 24 TC 3 Z9 3 U1 1 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1198 EP 1207 DI 10.1093/jee/tov052 PG 10 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000045 PM 26470246 ER PT J AU Frank, DL Kurtz, R Tinsley, NA Gassmann, AJ Meinke, LJ Moellenbeck, D Gray, ME Bledsoe, LW Krupke, CH Estes, RE Weber, P Hibbard, BE AF Frank, Daniel L. Kurtz, Ryan Tinsley, Nicholas A. Gassmann, Aaron J. Meinke, Lance J. Moellenbeck, Daniel Gray, Michael E. Bledsoe, Larry W. Krupke, Christian H. Estes, Ronald E. Weber, Patrick Hibbard, Bruce E. TI Effect of Seed Blends and Soil-Insecticide on Western and Northern Corn Rootworm Emergence from mCry3A+eCry3.1Ab Bt Maize SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; Diabrotica barberi; refuge-in-a-bag; MIR604; 5307; insect resistance management; seed mix refuge ID DIABROTICA-VIRGIFERA-VIRGIFERA; ERROR COMPONENT MODEL; COLEOPTERA-CHRYSOMELIDAE; TRANSGENIC-MAIZE; CRY34/35AB1 PROTEINS; RESISTANCE; LARVAE; FIELD; MANAGEMENT; MORTALITY AB Seed blends containing various ratios of transgenic Bt maize (Zea mays L.) expressing the mCry3A + eCry3.1Ab proteins and non-Bt maize (near-isoline maize) were deployed alone and in combination with a soil applied pyrethroid insecticide (Force CS) to evaluate the emergence of the western corn rootworm, Diabrotica virgifera virgifera LeConte, in a total of nine field environments across the Midwestern United States in 2010 and 2011. Northern corn rootworm, Diabrotica barberi Smith & Lawrence emergence was also evaluated in four of these environments. Both western and northern corn rootworm beetle emergence from all Bt treatments was significantly reduced when compared with beetle emergence from near-isoline treatments. Averaged across all environments, western corn rootworm beetle emergence from 95:5, 90:10, and 80:20 seed blend ratios of mCry3A + eCry3.1Ab: near-isoline were 2.6-, 4.2-, and 6.7-fold greater than that from the 100:0 ratio treatment. Northern corn rootworm emergence from the same seed blend treatments resulted in 2.8-, 3.2-, and 4.2-fold more beetles than from the 100:0 treatment. The addition of Force CS (tefluthrin) significantly reduced western corn rootworm beetle emergence for each of the three treatments to which it was applied. Force CS also significantly delayed the number of days to 50% beetle emergence in western corn rootworms. Time to 50% beetle emergence in the 100% mCry3A + eCry3.1Ab treatment with Force CS was delayed 13.7 d when compared with western corn rootworm beetle emergence on near-isoline corn. These data are discussed in terms of rootworm resistance management. C1 [Frank, Daniel L.; Hibbard, Bruce E.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Kurtz, Ryan] Syngenta Biotechnol Inc, Res Triangle Pk, NC 27709 USA. [Tinsley, Nicholas A.; Gray, Michael E.; Estes, Ronald E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Gassmann, Aaron J.; Weber, Patrick] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Meinke, Lance J.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Moellenbeck, Daniel] DM Crop Res Grp Inc, Polk City, IA 50226 USA. [Bledsoe, Larry W.; Krupke, Christian H.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. RP Hibbard, BE (reprint author), Univ Missouri, USDA ARS, Plant Genet Res Unit, 205 Curtis Hall, Columbia, MO 65211 USA. EM Bruce.Hibbard@ars.usda.gov FU Syngenta; Biotechnology Risk Assessment Grant Program from the USDA National Institute of Food and Agriculture [2009-33120-20256] FX We wish to thank a large number of workers at each location that assisted in the labor-intensive tasks of setting up plots, digging screen tents into the ground, and collecting, counting and sexing beetles. This study was funded, in part by Syngenta and in part by Biotechnology Risk Assessment Grant Program competitive grant 2009-33120-20256 from the USDA National Institute of Food and Agriculture. Finally, we wish to thank two anonymous reviewers whose suggestions improved the writing quality and overall manuscript. This article reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA) or any of the universities represented in this publication. USDA is an equal opportunity provider and employer. NR 41 TC 5 Z9 5 U1 1 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1260 EP 1270 DI 10.1093/jee/tov081 PG 11 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000053 PM 26470254 ER PT J AU Ruiz-Arce, R Owen, CL Thomas, DB Barr, NB McPheron, BA AF Ruiz-Arce, Raul Owen, Christopher L. Thomas, Donald B. Barr, Norman B. McPheron, Bruce A. TI Phylogeographic Structure in Anastrepha ludens (Diptera: Tephritidae) Populations Inferred With mtDNA Sequencing SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Anastrepha ludens; mitochondrial DNA; Mexican fruit fly; sequencing; COI ID COSTA-RICA; STATISTICAL TESTS; CERATITIS DIPTERA; SUSPENSA DIPTERA; DNA POLYMORPHISM; SPECIES DIPTERA; FRUIT-FLIES; EVOLUTIONARY; FRATERCULUS; SYSTEMATICS AB Anastrepha ludens (Loew) (Diptera: Tephritidae), the Mexican fruit fly, is a major pest of citrus and mango. It has a wide distribution in Mexico and Central America, with infestations occurring in Texas, California, and Florida with origins believed to have been centered in northeastern Mexico. This research evaluates the utility of a sequence-based approach for two mitochondrial (COI and ND6) gene regions. We use these markers to examine genetic diversity, estimate population structure, and identify diagnostic information for A. ludens populations. We analyzed 543 individuals from 67 geographic collections and found one predominant haplotype occurring in the majority of specimens. We observed 68 haplotypes in all and see differences among haplotypes belonging to northern and southern collections. Mexico haplotypes differ by few bases possibly as a result of a recent bottleneck event. In contrast to the hypothesis suggesting northeastern Mexico as the origin of this species, we see that specimens from two southern collections show high genetic variability delineating three mitochondrial groups. These data suggest that Central America is the origin for A. ludens. We show that COI and ND6 are useful for phylogeographic studies of A. ludens. C1 [Ruiz-Arce, Raul; Barr, Norman B.] USDA, APHIS Sci & Technol, Miss Lab, Moore Air Base, Edinburg, TX 78541 USA. [Ruiz-Arce, Raul; Owen, Christopher L.; McPheron, Bruce A.] Penn State Univ, Dept Entomol, University Pk, PA 16801 USA. [Owen, Christopher L.] George Washington Univ, Computat Biol Inst, Ashburn, VA 20147 USA. [Thomas, Donald B.] USDA ARS, Cattle Fever Tick Res Lab, Moore Air Base, Edinburg, TX 78541 USA. [McPheron, Bruce A.] Ohio State Univ, Coll Food Agr & Environm Sci, Columbus, OH 43210 USA. RP Ruiz-Arce, R (reprint author), USDA, APHIS Sci & Technol, Miss Lab, Moore Air Base, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. EM Raul.A.Ruiz@aphis.usda.gov OI McPheron, Bruce/0000-0001-9623-5100 FU United States Department of Agriculture-Animal Plant Health Inspection Service-Center for Plant Health Science & Technology Project [M6M02]; USDA/Penn State cooperative agreement [07-8100-1051-CA] FX We are grateful to Roxanne E. Farris, Rosita Deleon, Juan D. Vasquez, Lisa A. Ledezma, and Oscar Obregon at the USDA-APHIS-CPHST Mission Laboratory in Edinburg, TX, for providing DNA extractions, and to Samuel Gum and Oralia Ruiz for their technical assistance at Penn State University, University Park, PA. In addition, we thank Dr David W. Bartels at the USDA-APHIS-CPHST Mission Laboratory for map construction. We also thank Dr Deborah S. Grove (Director of the Genomics Core Facility), Ashley Price, and Sheila Plock for their assistance at the Pennsylvania State University-Huck Institute Nucleic Acid Facility. The use or mention of a trademark or proprietary product does not constitute an endorsement, guarantee, or warranty of the product and does not imply its approval to the exclusion of other suitable products by the U.S. Department of Agriculture, an equal opportunity employer. This work was supported by the United States Department of Agriculture-Animal Plant Health Inspection Service-Center for Plant Health Science & Technology Project Code#M6M02, USDA/Penn State cooperative agreement 07-8100-1051-CA. NR 47 TC 1 Z9 1 U1 1 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1324 EP 1336 DI 10.1093/jee/tov082 PG 13 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000060 PM 26470261 ER PT J AU Ramm, C Wachholtz, M Amundsen, K Donze, T Heng-Moss, T Twigg, P Palmer, NA Sarath, G Baxendale, F AF Ramm, Crystal Wachholtz, Michael Amundsen, Keenan Donze, Teresa Heng-Moss, Tiffany Twigg, Paul Palmer, Nathan A. Sarath, Gautam Baxendale, Fred TI Transcriptional Profiling of Resistant and Susceptible Buffalograsses in Response to Blissus occiduus (Hemiptera: Blissidae) Feeding SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE buffalograss; chinch bug; transcriptome; peroxidase; catalase ID GENE-EXPRESSION; DIFFERENTIAL EXPRESSION; FUNCTIONAL GENOMICS; PLANT-RESPONSES; DEFENSE; PEROXIDASES; TOLERANCE; APHIDIDAE; STRESS; HERBIVORES AB Understanding plant resistance mechanisms at a molecular level would provide valuable insights into the biological pathways impacted by insect feeding, and help explain specific plant tolerance mechanisms. As a first step in this process, we conducted next-generation sequencing using RNA extracted from chinch bug-tolerant and -susceptible buffalograss genotypes at 7 and 14 d after chinch bug feeding. Sequence descriptions and gene ontology terms were assigned to 1,701 differentially expressed genes. Defense-related transcripts were differentially expressed within the chinch bug-tolerant buffalograss, Prestige, and susceptible buffalograss, 378. Interestingly, four peroxidase transcripts had higher basal expression in tolerant control plants compared with susceptible control plants. Defense-related transcripts, including two peroxidase genes, two catalase genes, several cytochrome P450 transcripts, a glutathione s-transferase, and a WRKY gene were upregulated within the Prestige transcriptome in response to chinch bug feeding. The majority of observed transcripts with oxidoreductase activity, including nine peroxidase genes and a catalase gene, were downregulated in 378 in response to initial chinch bug feeding. The observed difference in transcript expression between these two buffalograss genotypes provides insight into the mechanism(s) of resistance, specifically buffalograss tolerance to chinch bug feeding. C1 [Ramm, Crystal; Donze, Teresa; Heng-Moss, Tiffany; Baxendale, Fred] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Wachholtz, Michael] Univ Nebraska, Dept Biol, Omaha, NE 68182 USA. [Amundsen, Keenan; Palmer, Nathan A.; Sarath, Gautam] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Twigg, Paul] Univ Nebraska, Dept Biol, Kearney, NE 68449 USA. [Palmer, Nathan A.; Sarath, Gautam] USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Heng-Moss, T (reprint author), Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. EM thengmoss2@unl.edu FU Nebraska Research Initiative Grant; United States Golf Association FX This research was supported in part by the Nebraska Research Initiative Grant and the United States Golf Association. NR 56 TC 3 Z9 3 U1 0 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1354 EP 1362 DI 10.1093/jee/tov067 PG 9 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000063 PM 26470264 ER PT J AU Wilson, BE Vanweelden, MT Beuzelin, JM Reagan, TE Way, MO White, WH Wilson, LT Showler, AT AF Wilson, Blake E. Vanweelden, Matthew T. Beuzelin, Julien M. Reagan, Thomas E. Way, Michael O. White, William H. Wilson, Lloyd T. Showler, Allan T. TI A Relative Resistance Ratio for Evaluation of Mexican Rice Borer (Lepidoptera: Crambidae) Susceptibility Among Sugarcane Cultivars SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Saccharum spp; Eoreuma loftini; cultivar resistance ID EOREUMA-LOFTINI; TEXAS SUGARCANE; PYRALIDAE; OVIPOSITION; REGISTRATION; MANAGEMENT; LOUISIANA; VARIETIES; DAMAGE AB The Mexican rice borer, Eoreuma loftini (Dyar), is a major pest of sugarcane (hybrids of Saccharum spp.) in Louisiana and Texas. Resistance to E. loftini was evaluated in 51 commercial and experimental cultivars of sugarcane, energycane (hybrids of Saccharum spp.), and sorghum [Sorghum bicolor (L.) Moench and hybrids of Sorghum spp.] in four replicated small plot field experiments from 2009 to 2012. A relative resistance ratio was developed to compare levels of susceptibility among cultivars based on the percentage of bored internodes and survival to adulthood. This index was able to separate cultivars into five resistance categories and provides a new method for comparing levels of resistance among cultivars. E. loftini pest pressure in 2009 was among the highest recorded with injury ranging from 55 to 88% bored internodes. Commercial sugarcane cultivar HoCP 85-845 was identified as resistant in three of four experiments, whereas HoCP 04-838 was identified as susceptible in all experiments. Of the five sugarcane cultivars in commercial production in the Rio Grande Valley of Texas, only TCP 87-3388 was categorized as resistant. Of the cultivars with potential for bioenergy production, all of the energycane cultivars demonstrated higher levels of resistance than high-biomass and sweet sorghum cultivars. Continued evaluation of cultivar resistance to E. loftini is important to development of effective integrated pest management strategies for this pest. C1 [Wilson, Blake E.; Vanweelden, Matthew T.; Reagan, Thomas E.] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [Beuzelin, Julien M.] Louisiana State Univ, Ctr Agr, Dean Lee Res Stn, Alexandria, LA 71302 USA. [Way, Michael O.; Wilson, Lloyd T.] Texas A&M AgriLife Res & Extens Ctr, Beaumont, TX 77713 USA. [White, William H.] USDA ARS, Sugar Cane Res Unit, Houma, LA 70360 USA. [Showler, Allan T.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Wilson, BE (reprint author), Louisiana State Univ, Ctr Agr, Dept Entomol, 404 Life Sci Bldg, Baton Rouge, LA 70803 USA. EM bwils26@lsu.edu FU USDA National Institute of Food and Agriculture (NIFA) bioenergy grant [2011-67009-30132]; American Sugar Cane League FX Mark Nunez, Rebecca Pearson, and other Texas A&M AgriLife Research Beaumont staff provided technical support. Randy Richard (USDA-ARS) assisted in obtaining the Louisiana cultivars and participated in planting and harvesting activities. Tony Prado with the Rio Grande Valley Sugar Growers, as well as Andy Scott and Eddie Hernandez of Rio Farms, provided Rio Grande Valley sugarcane cultivars. Kenneth Gravois provided cultivars from the LSU AgCenter breeding program. This work was supported in part by USDA National Institute of Food and Agriculture (NIFA) bioenergy grant 2011-67009-30132 and the American Sugar Cane League. This manuscript was approved by the director of the Louisiana Agricultural Experiment Station as manuscript no. 2014-234-15668. NR 39 TC 2 Z9 3 U1 1 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1363 EP 1370 DI 10.1093/jee/tov076 PG 8 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000064 PM 26470265 ER PT J AU Diaz-Montano, J Campbell, JF Phillips, TW Throne, JE AF Diaz-Montano, John Campbell, James F. Phillips, Thomas W. Throne, James E. TI Evaluation of Potential Attractants for Six Species of Stored-Product Psocids (Psocoptera: Liposcelididae, Trogiidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE booklice; attractant; stored product ID BOSTRYCHOPHILA BADONNEL PSOCOPTERA; POPULATION-GROWTH; ESSENTIAL OILS; COMPARATIVE REPELLENCY; CONTROLLED-ATMOSPHERE; SUBSTRATE SELECTION; GRAIN PROTECTANTS; PAETA PSOCOPTERA; BOOK-PAPER; WHEAT AB Psocids have emerged as worldwide pests of stored commodities during the past two decades, and are difficult to control with conventional management tactics such as chemical insecticides. Therefore, it is necessary to investigate alternative management strategies, such as the use of attractants for monitoring and controlling psocids, which can be incorporated into integrated pest management programs for psocids. Using a two-choice pitfall test, we studied the response of adults of different ages and sexes of Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae), Liposcelis paeta Pearman, Liposcelis decolor (Pearman), Liposcelis brunnea Motschulsky, Liposcelis corrodens (Heymons), and Lepinotus reticulatus Enderlein (Psocoptera: Trogiidae) to volatiles from different potential attractants including grains, grain-based oils, brewer's yeast, wheat germ, and commercially available kairomone lures. For all species tested, sex and age did not have a major influence on response to the different potential attractants. Brewer's yeast most consistently elicited the strongest response for psocids, but this response frequently was not different from that to wheat germ and wheat germ oil. The percentage response to brewer's yeast varied among the psocid species tested: L. decolor (73-78%), L. entomophila (62-73%), L. brunnea (64-68%), L. paeta (42-57%), Lep. reticulatus (40%), and L. corrodens (15-19%). Two psocids species (L. corrodens and Lep. reticulatus) had low responses to all the potential attractants evaluated compared with the other four species. These results show there is high potential for using these attractants in a psocid-monitoring program. C1 [Diaz-Montano, John; Campbell, James F.; Throne, James E.] ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect & Engn Res Unit, Manhattan, KS 66502 USA. [Phillips, Thomas W.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Diaz-Montano, J (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect & Engn Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM john.diaz-montano@ars.usda.gov FU Kansas Agricultural Experiment Station FX We thank Ann Redmon for excellent technical assistance. T.W.P. was supported by the Kansas Agricultural Experiment Station. NR 42 TC 1 Z9 1 U1 2 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1398 EP 1407 DI 10.1093/jee/tov028 PG 10 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000067 PM 26470268 ER PT J AU Kavallieratos, NG Athanassiou, CG Arthur, FH AF Kavallieratos, Nickolas G. Athanassiou, Christos G. Arthur, Frank H. TI Efficacy of Deltamethrin Against Stored-Product Beetles at Short Exposure Intervals or on a Partially Treated Rice Mass SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE deltamethrin; short exposure; partially treated rice; stored-product beetles ID METHYL PLUS DELTAMETHRIN; CHLORPYRIFOS-METHYL; GRAIN PROTECTANTS; WEEVILS COLEOPTERA; PROGENY PRODUCTION; BETA-CYFLUTHRIN; FIELD STRAINS; WHEAT; MAIZE; CURCULIONIDAE AB Stored-product insects can potentially be exposed to grain protectants for variable time periods. Adults of three species, the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae), the granary weevil Sitophilus granarius (L.) (Coleoptera: Curculionidae), and the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) were exposed for 1, 4, 8, and 24 h on brown rice treated with the pyrethroid deltamethrin at the label rate of 0.5 ppm, then removed and placed on untreated rice. Adults of these same species plus the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae), the maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), and the warehouse beetle, Trogoderma variabile Ballion (Coleoptera: Dermestidae) were exposed on treated brown rice mixed with varying amounts of untreated rice to assess progeny production. Immediate and delayed mortality of exposed adults did not exceed 7% for any exposure interval, but progeny production for T. castaneum was generally lower in comparison with that for the other species. Increasing the amount of treated rice decreased progeny production of R. dominica but not for any Sitophilus species. Mixed results were obtained for T. castaneum and T. variabile. Results show that long exposure times and treatment of an entire rice mass may be necessary to give complete control of stored-product beetles. C1 [Kavallieratos, Nickolas G.] Agr Univ Athens, Dept Crop Sci, Lab Agr Zool & Entomol, GR-11855 Athens, Greece. [Kavallieratos, Nickolas G.] Benaki Phytopathol Inst, Dept Entomol & Agr Zool, Lab Agr Entomol, Kifisia 14561, Attica, Greece. [Kavallieratos, Nickolas G.; Athanassiou, Christos G.; Arthur, Frank H.] Ctr Grain & Anim Hlth Res, USDA ARS, Manhattan, KS 66502 USA. [Athanassiou, Christos G.] Univ Thessaly, Dept Agr Crop Prod & Rural Dev, Lab Entomol & Agr Zool, Nea Ionia 38446, Magnissia, Greece. RP Kavallieratos, NG (reprint author), Agr Univ Athens, Dept Crop Sci, Lab Agr Zool & Entomol, 75 Iera Odos Str, GR-11855 Athens, Greece. EM nick_kaval@hotmail.com NR 27 TC 4 Z9 4 U1 1 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1416 EP 1421 DI 10.1093/jee/tov060 PG 6 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000069 PM 26470270 ER PT J AU Athanassiou, CG Hasan, MM Phillips, TW Aikins, MJ Throne, JE AF Athanassiou, Christos G. Hasan, M. Mahbub Phillips, Thomas W. Aikins, M. Jamie Throne, James E. TI Efficacy of Methyl Bromide for Control of Different Life Stages of Stored-Product Psocids SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE methyl bromide; fumigant; Psocoptera; Liposcelis; Lepinotus ID LIPOSCELIS-BOSTRYCHOPHILA; POPULATION-GROWTH; SULFURYL FLUORIDE; LEPINOTUS-RETICULATUS; GRAIN PROTECTANTS; PSOCOPTERA; PHOSPHINE; WHEAT; ENTOMOPHILA; FUMIGATION AB The psocid species Liposcelis paeta Pearman, Liposcelis entomophila (Enderlein), Liposcelis decolor (Pearman), Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae), and Lepinotus reticulatus Enderlein (Psocoptera: Trogiidae) were evaluated in laboratory bioassays to determine their susceptibility to six concentrations of methyl bromide (0.027, 0.113, 0.280, 0.393, 0.452, and 0.616 g/m(3)) after 48 h of exposure at 27.5 degrees C. The life stages that were evaluated were adults (for all species), nymphs (for all species except Lep. reticulatus), and eggs (for L. entomophila, L. decolor, and L. bostrychophila). Adults and nymphs were very susceptible, and complete mortality was recorded at concentrations between 0.027 and 0.280 g/m(3). In contrast, eggs were by far more tolerant than adults and nymphs for all species tested. At 0.027 g/m(3), mortality did not exceed 53%, while survival was high even at 0.113 g/m(3). Complete (100%) egg mortality was recorded at 0.393 g/m(3) for L. decolor and at 0.452 g/m(3) for L. entomophila and L. bostrychophila; concentrations estimated to give 99% mortality for eggs of these three species were 0.710, 1.044, and 0.891 g/m(3), respectively. These results show that stored-product psocids are susceptible to methyl bromide, but concentrations of >= 0.452 g/m(3) should be used to control all life stages. C1 [Athanassiou, Christos G.] Univ Thessaly, Dept Agr Crop Prod & Rural Environm, Lab Entomol & Agr Zool, Nea Ionia Magnesia 38446, Greece. [Athanassiou, Christos G.; Throne, James E.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Hasan, M. Mahbub; Phillips, Thomas W.; Aikins, M. Jamie] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Hasan, M. Mahbub] Rajshahi Univ, Dept Zool, Lab Post Harvest Entomol, Rajshahi 6205, Bangladesh. RP Athanassiou, CG (reprint author), Univ Thessaly, Dept Agr Crop Prod & Rural Environm, Lab Entomol & Agr Zool, Phytokou Str, Nea Ionia Magnesia 38446, Greece. EM athanassiou@agr.uth.gr FU Kansas Agricultural Experiment Station [15-237-J]; U.S. Department of Agriculture (USDA) Methyl Bromide Transitions program; Fulbright Foundation FX We would like to thank Ann Redmon and Ngunza Kisangani for technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture, the University of Thessaly, Kansas State University, or Rajshahi University. Partial funding for this work was provided by the Kansas Agricultural Experiment Station, for which this is manuscript 15-237-J, a grant from the U.S. Department of Agriculture (USDA) Methyl Bromide Transitions program, and support to M.M.H. from the Fulbright Foundation. USDA is an equal opportunity provider and employer. NR 24 TC 2 Z9 2 U1 2 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1422 EP 1428 DI 10.1093/jee/tov069 PG 7 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000070 PM 26470271 ER PT J AU Chaudhury, MF Chen, H Sagel, A Skoda, SR AF Chaudhury, M. F. Chen, H. Sagel, A. Skoda, S. R. TI Effects of New Dietary Ingredients Used in Artificial Diet for Screwworm Larvae (Diptera: Calliphoridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cochliomyia hominivorax; bovine blood; blood cell; insect nutrition; insect rearing ID COCHLIOMYIA-HOMINIVORAX; GELLED MEDIA; BLOOD; AGENT AB Spray-dried whole bovine blood, dry poultry egg, and a dry milk substitute are the constituents of the artificial diet currently used for mass rearing screwworm larvae, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae). Due to high cost and uncertainty of the commercial supply of spray-dried blood, research was conducted to identify alternative, locally available, inexpensive, dietary ingredients which could reduce cost of rearing and eliminate concerns of short supply. Experimental diets were prepared without blood component and with various ratios of bovine blood or blood cell product and defatted soy flour. Results indicate that spray-dried bovine blood can be replaced by a readily available and less expensive blood cell product. When the quantity of whole dried blood or blood cell component was reduced or removed completely from the diet, the larvae did not feed adequately, resulting in high mortality. Those larvae that survived produced pupae that were of unacceptable quality. When the milk product was replaced by soy flour, pupae were slightly smaller than those reared using the current diet; however, replacement of egg product with soy flour produced even smaller pupae. Longevity of adult flies that emerged from these small pupae was short and the females deposited few eggs. These results indicate that soy flour cannot replace the blood component from the diet, but can replace the milk product successfully. It is likely that some factor or a combination of factors in the blood act as feeding stimulants, without which larvae are unable to feed normally, resulting in high larval mortality. C1 [Chaudhury, M. F.; Chen, H.] UNLEC, USDA ARS, Screwworm Res Unit, Lincoln, NE 68583 USA. [Sagel, A.] USDA ARS, COPEG, Screwworm Res Unit, Pacora, Panama. [Skoda, S. R.] USDA ARS, Screwworm Res Unit, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Chaudhury, MF (reprint author), UNLEC, USDA ARS, Screwworm Res Unit, Entomol Hall, Lincoln, NE 68583 USA. EM Muhammad.Chaudhury@ars.usda.gov NR 25 TC 0 Z9 0 U1 1 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1429 EP 1434 DI 10.1093/jee/tov039 PG 6 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000071 PM 26470272 ER PT J AU Klick, J Yang, WQ Bruck, DJ AF Klick, J. Yang, W. Q. Bruck, D. J. TI Marking Drosophila suzukii (Diptera: Drosophilidae) With Rubidium or N-15 SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE rubidium; N-15; insect marking; strawberry; spotted wing drosophila ID PHYSIOLOGICAL MARKER; PHYTOPHAGOUS INSECTS; PEST-MANAGEMENT; FIELD; DISPERSAL; COTTON; CORN AB Drosophila suzukii Matsumura (Diptera: Drosophilidae) has caused significant economic damage to berry and stone fruit production regions. Markers that are systemic in plants and easily transferred to target organisms are needed to track D. suzukii exploitation of host resources and trophic interactions. High and low concentrations of the trace element, rubidium (Rb), and the stable isotope, N-15, were tested to mark D. suzukii larvae feeding on fruits of enriched strawberry plants grown in containers under greenhouse conditions. Fly marker content and proportion of flies marked 1, 7, and 14 d after emergence from enriched fruits and fly dry mass were analyzed. Nearly 100% of the flies analyzed 14 d after emerging from N-15-enriched plants were marked, whereas only 30-75% and 0-3% were marked 14 d after emerging from high and low Rb concentration plants, respectively. Rapid Rb decay, strong N-15 persistence, and the economics of using these markers in the field to elucidate D. suzukii pest ecology are discussed. C1 [Klick, J.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yang, W. Q.] Oregon State Univ, North Willamette Res & Extens Ctr, Dept Hort, Aurora, OR 97002 USA. [Bruck, D. J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Klick, J (reprint author), Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. EM jimmyklick@gmail.com FU Agricultural Research Foundation; USDA Specialty Crop Initiative Grant [2010-51181-21167]; USDA Current Research Information System [5358-22000-037-00D] FX We acknowledge Molly Albrecht, Kelly Donahue, Amanda Lake, and Scott Shane for technical assistance. Thanks to Jadwiga Giebultowicz, George Hoffman, Joe Kleiber, Gail Langellotto-Rhodaback, Jana Lee, and Bob Van Steenwyk for helpful discussions; and Amy Dreves for reviewing an early draft of this manuscript. We are grateful to Suean Ott and Carolyn Scagel for digesting Rb samples and Andy Ungerer and the Oregon State University (OSU) W.M. Keck Collaboratory for ICP-MS assistance. We appreciate the help of David Myrold and the OSU Stable Isotope Research Unit for 15N analysis. Thanks to Hannah Tavalire for statistical consultation and manuscript review. Funding was provided by the Agricultural Research Foundation, USDA Specialty Crop Initiative Grant 2010-51181-21167, and USDA Current Research Information System 5358-22000-037-00D. NR 30 TC 0 Z9 0 U1 2 U2 16 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1447 EP 1451 DI 10.1093/jee/tov007 PG 5 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000074 PM 26470275 ER PT J AU Elliott, NC Backoulou, GF Brewer, MJ Giles, KL AF Elliott, N. C. Backoulou, G. F. Brewer, M. J. Giles, K. L. TI NDVI to Detect Sugarcane Aphid Injury to Grain Sorghum SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Homoptera; Aphididae; population; monitoring ID HOMOPTERA; WHEAT; STRESS; COTTON AB Multispectral remote sensing has potential to provide quick and inexpensive information on sugarcane aphid, Melanaphis sacchari (Zehntner), pest status in sorghum fields. We describe a study conducted to determine if injury caused by sugarcane aphid to sorghum plants in fields of grain sorghum could be detected using multispectral remote sensing from a fixed wing aircraft. A study was conducted in commercial grain sorghum fields in the Texas Gulf Coast region in June 2014. Twenty-six commercial grain sorghum fields were selected and rated for the level of injury to sorghum plants in the field caused by sugarcane aphid. Plant growth stage ranged from 5.0 (watery ripe) to 7.0 (hard dough) among fields; and plant injury rating from sugarcane aphid ranged from 1.0 (little or no injury) to 4.0 (> 40% of plants displaying injury) among fields. The normalized differenced vegetation index (NDVI) is calculated from light reflectance in the red and near-infrared wavelength bands in multispectral imagery and is a common index of plant stress. High NDVI indicates low levels of stress and low NDVI indicates high stress. NDVI ranged from -0.07 to 0.26 among fields. The correlation between NDVI and plant injury rating was negative and significant, as was the correlation between NDVI and plant growth stage. The negative correlation of NDVI with injury rating indicated that plant stress increased with increasing plant injury. Reduced NDVI with increasing plant growth probably resulted from reduced photosynthetic activity in more mature plants. The correlation between plant injury rating and plant growth stage was positive and significant indicating that plant injury from sugarcane aphid increased as plants matured. The partial correlation of NDVI with plant injury rating was negative and significant indicating that NDVI decreased with increasing plant injury after adjusting for its association with plant growth stage. We demonstrated that remotely sensed imagery acquired from grain sorghum fields using an airborne multi-spectral imaging system was sensitive to injury to sorghum plants caused by sugarcane aphid. C1 [Elliott, N. C.] USDA ARS, Plant Sci Res Lab, Stillwater, OK 74075 USA. [Backoulou, G. F.; Giles, K. L.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [Brewer, M. J.] Texas AgriLife Res & Extens Ctr Corpus Christi, Corpus Christi, TX 78406 USA. RP Elliott, NC (reprint author), USDA ARS, Plant Sci Res Lab, 1301 N Western Rd, Stillwater, OK 74075 USA. EM norman.elliott@ars.usda.gov NR 11 TC 2 Z9 2 U1 7 U2 35 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2015 VL 108 IS 3 BP 1452 EP 1455 DI 10.1093/jee/tov080 PG 4 WC Entomology SC Entomology GA CK5CF UT WOS:000356239000075 PM 26470276 ER PT J AU Ham, BR Myrick, CA Barrows, FT Yeoman, CJ Duff, GC Maskill, MG Sealey, WM AF Ham, Brian R. Myrick, Christopher A. Barrows, Frederic T. Yeoman, Carl J. Duff, Glenn C. Maskill, Mark G. Sealey, Wendy M. TI Feed Characteristics Alter Growth Efficiency of Cutthroat Trout SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE cutthroat trout; feed type; Snake River cutthroat ID SALMON SALMO-SALAR; ONCORHYNCHUS-MYKISS; ATLANTIC SALMON; PERFORMANCE; DIETS; RESPONSES; YELLOWSTONE; FRY AB Hatchery-cultured cutthroat trout Oncorhynchus clarkii fed some commercially available rainbow trout feeds display slow growth and increased mortality. Feed characteristics such as buoyancy and texture alter feed acceptance in some fish species, but their effects have not been adequately addressed in cutthroat trout. Therefore, the objective of this study was to examine whether feed structure and behavior preferences explain the decreased hatchery performance of juvenile cutthroat trout. To achieve this, we conducted two feeding trials in which we fed Westslope cutthroat trout O. clarkii lewisi and Snake River finespotted cutthroat trout O. clarkii behnkei a single diet formulation manufactured to display four different characteristics (floating, sinking, semimoist pellets, or a flake feed) and compared consumption, weight gain, and survival. In the first feeding trial, we stocked Westslope cutthroat trout (initial weight 11.3 g +/- 0.5 g) at 20 fish/tank. We used two different sizes of tanks, with four replicate small tanks (54-L) and two replicate large tanks (96-L) per feed type. Results of the first trial demonstrated a significant effect of feed type but not tank size on weight gain of Westslope cutthroat trout with no interaction. Westslope cutthroat trout fed the flake feed gained less weight than did fish fed any of the other feed types. Feed conversion ratio was affected by both feed type and tank size with no interaction. In a second feeding trial, Snake River cutthroat trout (initial weight 19.5 g +/- 0.5 g) were stocked at 20 fish/tank in 96-L tanks with four replicate tanks per feed type. Results of the second trial demonstrated that Snake River cutthroat trout fed the flake feed grew less, had higher feed conversion ratio, elevated hepatosomatic index, and reduced muscle ratio compared with fish fed the other feeds. Results demonstrate that flake feeds are not adequate for cutthroat trout at this life stage. However, additional research is needed to address other culture-related limitations because only minor differences between fish fed other feed types were detected. C1 [Ham, Brian R.; Sealey, Wendy M.] US Fish & Wildlife Serv, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Myrick, Christopher A.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Barrows, Frederic T.] USDA ARS, Bozeman Fish Technol Ctr, Trout Grains Project, Bozeman, MT 59715 USA. [Yeoman, Carl J.; Duff, Glenn C.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USA. [Maskill, Mark G.] US Fish & Wildlife Serv, Creston Natl Fish Hatchery, Kalispell, MT 59901 USA. RP Sealey, WM (reprint author), US Fish & Wildlife Serv, Bozeman Fish Technol Ctr, 4050 Bridger Canyon Rd, Bozeman, MT 59715 USA. EM wendy_sealey@fws.gov FU Western Regional Aquaculture Center from the U.S. Department of Agriculture National Institute of Food and Agriculture [2012-38500-19657] FX Funding for the study was provided, in part, by the Western Regional Aquaculture Center through grant number 2012-38500-19657 from the U.S. Department of Agriculture National Institute of Food and Agriculture. NR 28 TC 0 Z9 0 U1 3 U2 7 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2015 VL 6 IS 1 BP 83 EP 91 DI 10.3996/052014-JFWM-042 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CL0JD UT WOS:000356627400007 ER PT J AU Singh, M Byars, JA Liu, SX AF Singh, Mukti Byars, Jeffrey A. Liu, Sean X. TI Navy Bean Flour Particle Size and Protein Content Affect Cake Baking and Batter Quality SO JOURNAL OF FOOD SCIENCE LA English DT Article DE cake batter rheology; cake baking; gluten-free; navy bean flour; protein ID COMPOSITE FLOURS; PULSE FLOURS; RHEOLOGICAL PROPERTIES; BREAD QUALITY; WHEAT-FLOUR; PEA FLOUR; CHICKPEA; LAYER; FUNCTIONALITY; FRACTIONS AB Whole navy bean flour and its fine and coarse particle size fractions were used to completely replace wheat flour in cakes. Replacement of wheat flour with whole bean flour significantly increased the protein content. The protein content was adjusted to 3 levels with navy bean starch. The effect of navy bean flour and its fractions at 3 levels of protein on cake batter rheology and cake quality was studied and compared with wheat flour samples. Batters prepared from navy bean flour and its fractions had higher viscosity than the cake flour. Reducing the protein content by addition of starch significantly lowered the viscosity of cake batters. The whole navy bean flour and coarse bean fraction cakes were softer than cakes made with wheat flour but had reduced springiness. Principal component analysis showed a clear discrimination of cakes according to protein. It also showed that low protein navy bean flour cakes were similar to wheat flour cakes. Navy bean flour with protein content adjusted to the level of cake (wheat) flour has potential as a healthy alternative in gluten-free cakes. Practical Application This research determined that by lowering the protein content of whole navy bean flour with addition of navy bean starch, navy bean flour could be used a healthy alternative in gluten-free cakes. There is a great demand for wheat alternatives in foods particularly baked goods as gluten sensitivity increases. Baked goods such as cakes have wheat flour as a major ingredient, which is rich in gluten protein. Bean proteins do not have gluten, and are a good source of soluble fiber, B-vitamins, iron, calcium, potassium, and phosphorous, and low in sodium and fat. Blends of whole navy bean flour with navy bean starch were prepared to adjust protein content. The blends were used to measure cake batter and cake quality. Cakes using navy bean flour blends, and wheat flour as a control were prepared. Standard methods were used to measure the batter and cake quality of control and test samples. These findings will benefit the pulse industry and promote health and wellness by creating healthy alternatives for wheat flour in healthy gluten-free cakes. C1 [Singh, Mukti; Byars, Jeffrey A.; Liu, Sean X.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Singh, M (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM mukti.singh@ars.usda.gov NR 34 TC 1 Z9 1 U1 2 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD JUN PY 2015 VL 80 IS 6 BP E1229 EP E1234 DI 10.1111/1750-3841.12869 PG 6 WC Food Science & Technology SC Food Science & Technology GA CK6YQ UT WOS:000356375600014 PM 25922214 ER PT J AU Wilson, EM Johanningsmeier, SD Osborne, JA AF Wilson, Emily M. Johanningsmeier, Suzanne D. Osborne, Jason A. TI Consumer Acceptability of Cucumber Pickles Produced by Fermentation in Calcium Chloride Brine for Reduced Environmental Impact SO JOURNAL OF FOOD SCIENCE LA English DT Article DE calcium chloride taste; cucumber pickles; detection threshold; sodium-free fermentation; sustainability; vegetable preservation ID POLYGALACTURONASE; SALTS; FRUIT; CRISPNESS; QUALITY; TASTE AB Fermentation of cucumbers in calcium chloride (CaCl2) brine has been proposed as an alternative process to reduce the environmental impact of traditional, high salt fermentations. The objective of this research was to determine whether consumer acceptability of pickle products would be impacted by fermentation and storage of cucumbers in CaCl2 brine. Cucumbers were fermented and stored with 0.1M CaCl2 or 1M sodium chloride (NaCl) in open-air, 3000 gal tanks at a commercial facility and processed into hamburger dill chips containing 0.38M NaCl. Cucumbers fermented in CaCl2 required additional desalting to reduce CaCl2 concentrations to that of current products. Consumers (n = 101) showed no significant preference for pickles from different fermentation treatments, whether stored for 2 mo (P = 0.75) or 8 mo (P = 0.68) prior to processing. In contrast, NaCl fermented pickles were preferred over CaCl2 fermented pickles stored for 10 mo and desalted only once (P < 0.01). A series of preference tests indicated that the taste of CaCl2 was not the factor affecting consumer preference, and the 50% detection threshold of CaCl2 in dill pickle chips was found to be 61.8 7.6 mM, indicating that processors could potentially use CaCl2 fermentations with a single desalting step. Consumer liking of flavor (n = 73) was not influenced by fermentation in CaCl2 or by 23 or 35 mM CaCl2 in finished products (P > 0.05), but variability in texture decreased consumer liking (P < 0.05). Although promising, individual fermentation variability and texture quality of CaCl2 fermented products should be further evaluated prior to broad implementation of this process. Practical Application Disposal of high salt waste continues to burden the pickled vegetable industry. Consumer acceptability of pickles produced by commercial fermentation and storage in 1.1% calcium chloride (CaCl2) brines and traditional 6% sodium chloride brines was evaluated. In 2 independent trials, consumers displayed no difference in preference for pickles produced from either process. Further investigation of the taste of CaCl2 in finished products suggested that processors may use the CaCl2 fermentation with their normal process without significant concern for alterations in the taste profile. However, variability in texture quality impacted consumer acceptability, indicating the need for further process optimization. C1 [Wilson, Emily M.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Johanningsmeier, Suzanne D.] N Carolina State Univ, USDA, ARS, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. [Osborne, Jason A.] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. RP Johanningsmeier, SD (reprint author), N Carolina State Univ, USDA, ARS, SAA Food Sci Res Unit, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM Suzanne.johanningsmeier@ars.usda.gov FU Pickle Packers Intl., Inc. FX The authors thank Mount Olive Pickle Company and Lisa Moeller for donation of resources and expertise, Pickle Packers Intl., Inc., for partial financial support, Lisa Rosenberg for assistance in performing sensory tests, D. Seth Fornea for texture and color analysis of selected samples, and Janet Hayes for microbiological testing of products prior to consumption. NR 27 TC 1 Z9 1 U1 9 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD JUN PY 2015 VL 80 IS 6 BP S1360 EP S1367 DI 10.1111/1750-3841.12882 PG 8 WC Food Science & Technology SC Food Science & Technology GA CK6YQ UT WOS:000356375600033 PM 25981784 ER PT J AU Cornwell, ER Anderson, GB Coleman, D Getchell, RG Groocock, GH Warg, JV Cruz, AM Casey, JW Bain, MB Bowser, PR AF Cornwell, Emily R. Anderson, Gregory B. Coleman, Destiny Getchell, Rodman G. Groocock, Geoffrey H. Warg, Janet V. Cruz, Angela M. Casey, James W. Bain, Mark B. Bowser, Paul R. TI Applying multi-scale occupancy models to infer host and site occupancy of an emerging viral fish pathogen in the Great Lakes SO JOURNAL OF GREAT LAKES RESEARCH LA English DT Article DE Multi-scale occupancy model; Reversible Jump Markov chain Monte Carlo; qRT-PCR; Viral hemorrhagic septicemia virus; Viral surveillance; Virus isolation ID HEMORRHAGIC-SEPTICEMIA-VIRUS; ST-LAWRENCE-RIVER; DETECTION PROBABILITIES; CELL-CULTURE; FRESH-WATER; VHSV-IVB; RT-PCR; PREVALENCE; INFECTION; DISEASES AB Emerging pathogens in wildlife are being described at an increasing rate, but the methods used to describe their dynamics in wildlife populations have been slow to develop. Understanding pathogen prevalence and risk factors for infection are critical first components of developing wildlife disease management However, the estimation of these attributes can be biased by imperfect detection of the pathogen. In this study, we adopt a multi-scale site occupancy model to estimate the probability of pathogen detection when the diagnostic test is imperfect, host detection is imperfect, and detection can be measured at multiple scales. In addition, this model allowed the comparison of different diagnostic tests for pathogen presence. We then applied this model to the detection of viral hemorrhagic septicemia virus (VHSV) in wild fish populations in the Great Lakes. We show that VHSV is still widely distributed in the Great Lakes, and that a multi-scale model can identify additional risk factors to those identified by previous logistic regression approaches. We also estimate detection probabilities using molecular and traditional virological methods. Although our approach has several limitations, it has important implications in the management and modeling of VHSV and other emerging pathogens in aquatic wildlife. (C) 2015 International Association for Great Lakes Research. Published by Elsevier B.V. All rights reserved. C1 [Cornwell, Emily R.; Coleman, Destiny; Getchell, Rodman G.; Groocock, Geoffrey H.; Casey, James W.; Bowser, Paul R.] Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Aquat Anim Hlth Program, Ithaca, NY 14853 USA. [Anderson, Gregory B.; Bain, Mark B.] Cornell Univ, Dept Nat Resources, Ithaca, NY 14853 USA. [Warg, Janet V.; Cruz, Angela M.] USDA, Natl Vet Serv Labs, VS, APHIS, Ames, IA 50010 USA. RP Cornwell, ER (reprint author), Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Aquat Anim Hlth Program, Ithaca, NY 14853 USA. EM erc58@cornell.edu FU United States Department of Agriculture Animal and Plant Health Inspection Service [10-9100-1294-GR] FX We thank Emily Nash, Deidre Hayward, and Geofrey Eckerlin for assistance in collecting fish; Sandra LaBuda, Po Ting Wong, Rebecca Fellman, Chelsea Bellmund, Lindsay Glasner, Phoebe Clark, and Jimmy Todhunter for assistance with fish dissections; Greg Wooster for technical assistance; and Dr. John Farrell and the SUNY ESF Thousand Islands Biological Station for field and sampling support. Funding was provided by the United States Department of Agriculture Animal and Plant Health Inspection Service Cooperative Agreement10-9100-1294-GR. NR 45 TC 4 Z9 4 U1 0 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0380-1330 J9 J GREAT LAKES RES JI J. Gt. Lakes Res. PD JUN PY 2015 VL 41 IS 2 BP 520 EP 529 DI 10.1016/j.jglr.2015.01.002 PG 10 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CK7GE UT WOS:000356400000024 ER PT J AU Eizenga, GC Jia, MH Pinson, SR Gasore, ER Prasad, B AF Eizenga, G. C. Jia, M. H. Pinson, S. R. Gasore, E. R. Prasad, B. TI Exploring sheath blight quantitative trait loci in a Lemont/O. meridionalis advanced backcross population SO MOLECULAR BREEDING LA English DT Article DE Oryza sativa; Rhizoctonia solani; Advanced backcross; QTL mapping; Asian rice; Oryza meridionalis; Sheath blight ID ORYZA-SATIVA L.; RICE GERMPLASM LINES; YIELD-RELATED TRAITS; RHIZOCTONIA-SOLANI; CULTIVATED RICE; GENUS ORYZA; PHYLOGENETIC-RELATIONSHIPS; DISEASE RESISTANCE; JAPONICA RICE; QTL-ANALYSIS AB Oryza meridionalis is the wild Oryza species endemic to Australia. There are eight AA-genome Oryza species, one of which is cultivated rice, O. sativa, and O. meridionalis is the most genetically distant one. An O. meridionalis (IRGC105608) accession, previously identified as being moderately resistant to rice sheath blight (ShB) disease, was used as the donor parent to develop an advanced backcross population with the US semidwarf rice cultivar, Lemont, as the recurrent parent. The population was genotyped with 113 DNA markers and a linkage map constructed that spanned 1234.5 cM. ShB disease was evaluated in both greenhouse and field conditions. Days to heading (DH), plant height (PH) and culm angle (CA) were recorded because they can confound ShB disease ratings under field conditions. Multiple interval mapping identified qShB9-2 as the ShB-QTL most consistently effective across the generations and variable evaluation environments, with resistance to ShB disease attributed to the O. meridionalis allele in all cases. No QTL for any of the potentially confounding traits was identified in this region of chromosome9 of this cross. Lemont was found to contribute the desirable alleles that decreased the DH on chromosome 8 and PH on chromosome 1, in part due to the semidwarf gene, sd1, for short plant stature. No CA-QTL was identified due to the lack of segregation for this trait, especially in the advanced backcross generations. Further studies will be undertaken to fine map the qShB9-2 region and identify linked markers for use in cultivar development. C1 [Eizenga, G. C.; Jia, M. H.; Pinson, S. R.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Gasore, E. R.; Prasad, B.] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. RP Eizenga, GC (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130 E, Stuttgart, AR 72160 USA. EM georgia.eizenga@ars.usda.gov FU USDA Cooperative State Research, Education and Extension Service-National Research Initiative [2004-35317-14867] FX This work was supported in part by the USDA Cooperative State Research, Education and Extension Service-National Research Initiative-Applied Plant Genomics Program entitled 'RiceCAP: A coordinated research, education, and extension project for the application of genomic discoveries to improve rice in the United States' (USDA/CSREES Grant 2004-35317-14867). The superb technical assistance of Quynh P. H. Grunden and student intern, Robert G. Floyd, at the Dale Bumpers National Rice Research Center is also acknowledged. We acknowledge Dr. Fleet N. Lee, Dr. Yeshii Wamishe and Scott Belmar (Univ. Arkansas, Rice Research and Extension Center) for technical assistance with field testing and inoculum preparation. NR 96 TC 0 Z9 0 U1 2 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD JUN PY 2015 VL 35 IS 6 AR 140 DI 10.1007/s11032-015-0332-3 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CK6AO UT WOS:000356310400011 ER PT J AU Guan, YZ Peace, C Rudell, D Verma, S Evans, K AF Guan, Yingzhu Peace, Cameron Rudell, David Verma, Sujeet Evans, Kate TI QTLs detected for individual sugars and soluble solids content in apple SO MOLECULAR BREEDING LA English DT Article DE Fruit quality; Marker-assisted breeding; RosBREED; SNP; Soluble solids content ID X-DOMESTICA BORKH.; FRUIT-QUALITY TRAITS; MALUS-PUMILA MILL.; PERSICA L. BATSCH; MOLECULAR MARKERS; CANDIDATE GENES; STORED APPLES; SWEET CHERRY; IDENTIFICATION; PRUNUS AB Sweetness is one of the most important fruit quality traits in breeding programs, determining the overall quality and flavor perception of apples. Selecting for this trait using conventional breeding methods is challenging due to the complexity of its genetic control. In order to improve the efficiency of trait selection via DNA-based markers, extensive studies focused on the detection of quantitative trait loci (QTL) and the development of DNA-based markers associated with QTL regions for traits of interest. Newly discovered QTLs detected in multiple apple breeding populations are presented here for individual sugars (fructose, glucose, sucrose, and sorbitol) and soluble solids content (SSC) at harvest, after 10, and 20 weeks of refrigerated storage followed by 1 week at room temperature in two successive years. A total of 1416 polymorphic SNPs were filtered from the RosBreed Apple SNP Infinium (R) array for QTL analysis using FlexQTL (TM) software. QTLs for individual sugars were identified on linkage groups (LG) 1, 2, 3, 4, 5, 9, 11, 12, 13, 15, and 16, and QTLs for SSC were found on LGs 2, 3, 12, 13, and 15. One QTL region on LG 1 was consistently identified for both fructose and sucrose from harvest through storage in both years, which accounted for 34-67 and 13-41 % of total phenotypic variation, respectively. These stable QTLs with high explained phenotypic variation on LG 1 for fructose content indicate a promising genomic region for DNA-based marker development to enable marker-assisted breeding for sweetness selection in apple breeding programs. C1 [Guan, Yingzhu; Evans, Kate] Washington State Univ, Tree Fruit Res & Extens Ctr, Dept Hort, Wenatchee, WA 98801 USA. [Peace, Cameron; Verma, Sujeet] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Rudell, David] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Evans, K (reprint author), Washington State Univ, Tree Fruit Res & Extens Ctr, Dept Hort, Wenatchee, WA 98801 USA. EM kate_evans@wsu.edu FU USDA's National Institute of Food and Agriculture-Specialty Crop Research Initiative [2009-51181-05808] FX This work was partially funded by USDA's National Institute of Food and Agriculture-Specialty Crop Research Initiative project, 'RosBREED: enabling marker-assisted breeding in Rosaceae' (2009-51181-05808). We thank Nancy Buchanan, Julia Harshman, Lisa Brutcher, and Bonnie Konishi for assistance in phenotyping, Dave Buchanan for his skillful experience on GC-MS, Jerry Tangren for his assistance with statistical analysis of phenotypic data, Umesh Rosyara for his assistance in QTL analysis, Marco Bink and Eric van de Weg for training in Pedigree-Based Analysis concepts and software. NR 70 TC 8 Z9 8 U1 8 U2 49 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD JUN PY 2015 VL 35 IS 6 AR 135 DI 10.1007/s11032-015-0334-1 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CK6AO UT WOS:000356310400013 ER PT J AU Woodard, SH Lozier, JD Goulson, D Williams, PH Strange, JP Jha, S AF Woodard, S. Hollis Lozier, Jeffrey D. Goulson, David Williams, Paul H. Strange, James P. Jha, Shalene TI Molecular tools and bumble bees: revealing hidden details of ecology and evolution in a model system SO MOLECULAR ECOLOGY LA English DT Review DE behavior; social evolution; ecology; genomics; insects; molecular evolution; population genetics ID COMPLEMENTARY SEX DETERMINATION; DIPLOID MALE PRODUCTION; DEFORMED-WING-VIRUS; ROYAL JELLY PROTEIN; BOMBUS-TERRESTRIS; HYMENOPTERA-APIDAE; GENE-EXPRESSION; HONEY-BEE; FORAGING DISTANCE; NEST DENSITY AB Bumble bees are a longstanding model system for studies on behaviour, ecology and evolution, due to their well-studied social lifestyle, invaluable role as wild and managed pollinators, and ubiquity and diversity across temperate ecosystems. Yet despite their importance, many aspects of bumble bee biology have remained enigmatic until the rise of the genetic and, more recently, genomic eras. Here, we review and synthesize new insights into the ecology, evolution and behaviour of bumble bees that have been gained using modern genetic and genomic techniques. Special emphasis is placed on four areas of bumble bee biology: the evolution of eusociality in this group, population-level processes, large-scale evolutionary relationships and patterns, and immunity and resistance to pesticides. We close with a prospective on the future of bumble bee genomics research, as this rapidly advancing field has the potential to further revolutionize our understanding of bumble bees, particularly in regard to adaptation and resilience. Worldwide, many bumble bee populations are in decline. As such, throughout the review, connections are drawn between new molecular insights into bumble bees and our understanding of the causal factors involved in their decline. Ongoing and potential applications to bumble bee management and conservation are also included to demonstrate how genetics- and genomics-enabled research aids in the preservation of this threatened group. C1 [Woodard, S. Hollis; Jha, Shalene] Univ Texas, Dept Integrat Biol, Austin, TX 78712 USA. [Woodard, S. Hollis] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Lozier, Jeffrey D.] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35401 USA. [Goulson, David] Univ Sussex, Sch Life Sci, Evolut Behav & Environm, Falmer BN1 9QG, E Sussex, England. [Williams, Paul H.] Nat Hist Museum, Dept Life Sci, London SW7 5BD, England. [Strange, James P.] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. RP Jha, S (reprint author), Univ Texas, Dept Integrat Biol, Austin, TX 78712 USA. EM sjha@austin.utexas.edu RI Goulson, Dave/A-2462-2010 OI Goulson, Dave/0000-0003-4421-2876 FU USDA-NIFA FX We thank A. Bourke and two anonymous reviewers for their insightful reviews and S. Barribeau and B.M. Sadd for sharing early versions of their manuscripts. This work was supported by a USDA-NIFA Postdoctoral Fellowship awarded to S.H.W. NR 235 TC 4 Z9 4 U1 31 U2 122 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD JUN PY 2015 VL 24 IS 12 BP 2916 EP 2936 DI 10.1111/mec.13198 PG 21 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CL0FI UT WOS:000356615200004 PM 25865395 ER PT J AU Yan, L Graef, GL Nielsen, FH Johnson, LK Cao, J AF Yan, Lin Graef, George L. Nielsen, Forrest H. Johnson, LuAnn K. Cao, Jay TI Soy protein is beneficial but high-fat diet and voluntary running are detrimental to bone structure in mice SO NUTRITION RESEARCH LA English DT Article DE Bone; Fat; Running; Soy; Mice ID RESISTANT ACID-PHOSPHATASE; PHYSICAL-ACTIVITY; MINERAL DENSITY; INDUCED OBESITY; CORTICAL BONE; RAT MODEL; MASS; EXERCISE; OSTEOPOROSIS; WOMEN AB Physical activity and soy protein isolate (SPI) augmentation have been reported to be beneficial for bone health. We hypothesized that combining voluntary running and SPI intake would alleviate detrimental changes in bone induced by a high-fat diet. A 2 x 2 x 2 experiment was designed with diets containing 16% or 45% of energy as corn oil and 20% SPI or casein fed to sedentary or running male C57B1J6 mice for 14 weeks. Distal femurs were assessed for microstructural changes. The high-fat diet significantly decreased trabecular number (Tb.N) and bone mineral density (BMD) and increased trabecular separation (Tb.Sp). Soy protein instead of casein, regardless of fat content, in the diet significantly increased bone volume fraction, Tb.N, connectivity density, and BMD and decreased Tb.Sp. Voluntary running, regardless of fat content, significantly decreased bone volume fraction, Tb.N, connectivity density, and BMD and increased Tb.Sp. The high-fat diet significantly decreased osteocalcin and increased tartrate-resistant acid phosphatase 5b (TRAP 5b) concentrations in plasma. Plasma concentrations of osteocalcin were increased by both SPI and running. Running alleviated the increase in TRAP 5b induced by the high-fat diet. These findings demonstrate that a high-fat diet is deleterious, and SPI is beneficial to trabecular bone properties. The deleterious effect of voluntary running on trabecular structural characteristics indicates that there may be a maximal threshold of running beyond which beneficial effects cease and detrimental effects occur. Increases in plasma osteocalcin and decreases in plasma TRAP 5b in running mice suggest that a compensatory response occurs to counteract the detrimental effects of excessive running. Published by Elsevier Inc. C1 [Yan, Lin; Nielsen, Forrest H.; Johnson, LuAnn K.; Cao, Jay] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Graef, George L.] Univ Nebraska, Dept Agron, Lincoln, NE 68583 USA. RP Yan, L (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58202 USA. EM lin.yan@ars.usda.gov OI yan, lin/0000-0003-3646-6374 FU US Department of Agriculture, ARS [5450-51000-045-00D]; Nebraska Soybean Board (Lincoln, NE, USA) FX This work was supported by the US Department of Agriculture, ARS, research project 5450-51000-045-00D and by the Nebraska Soybean Board (Lincoln, NE, USA). NR 37 TC 1 Z9 1 U1 1 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUN PY 2015 VL 35 IS 6 BP 523 EP 531 DI 10.1016/j.nutres.2015.04.012 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CL0PA UT WOS:000356643400008 PM 25957968 ER PT J AU DeBlasio, SL Johnson, R Sweeney, MM Karasev, A Gray, SM MacCoss, MJ Cilia, M AF DeBlasio, Stacy L. Johnson, Richard Sweeney, Michelle M. Karasev, Alexander Gray, Stewart M. MacCoss, Michael J. Cilia, Michelle TI Potato leafroll virus structural proteins manipulate overlapping, yet distinct protein interaction networks during infection SO PROTEOMICS LA English DT Article DE Affinity purification MS; Luteoviridae, Polerovirus; Plant proteomics; Potato leafroll virus; Readthrough protein ID WESTERN YELLOWS VIRUS; TOBACCO-MOSAIC-VIRUS; PURIFICATION-MASS SPECTROMETRY; MINOR CAPSID PROTEIN; VIRAL COAT PROTEIN; READTHROUGH PROTEIN; APHID TRANSMISSION; MYZUS-PERSICAE; ARABIDOPSIS-THALIANA; DWARF LUTEOVIRUS AB Potato leafroll virus (PLRV) produces a readthrough protein (RTP) via translational readthrough of the coat protein amber stop codon. The RTP functions as a structural component of the virion and as a nonincorporated protein in concert with numerous insect and plant proteins to regulate virus movement/transmission and tissue tropism. Affinity purification coupled to quantitative MS was used to generate protein interaction networks for a PLRV mutant that is unable to produce the read through domain (RTD) and compared to the known wild-type PLRV protein interaction network. By quantifying differences in the protein interaction networks, we identified four distinct classes of PLRV-plant interactions: those plant and nonstructural viral proteins interacting with assembled coat protein (category I); plant proteins in complex with both coat protein and RTD (category II); plant proteins in complex with the RTD (category III); and plant proteins that had higher affinity for virions lacking the RTD (category IV). Proteins identified as interacting with the RTD are potential candidates for regulating viral processes that are mediated by the RTP such as phloem retention and systemic movement and can potentially be useful targets for the development of strategies to prevent infection and/or viral transmission of Luteoviridae species that infect important crop species. C1 [DeBlasio, Stacy L.; Sweeney, Michelle M.; Cilia, Michelle] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [DeBlasio, Stacy L.; Gray, Stewart M.; Cilia, Michelle] USDA ARS, Ithaca, NY 14853 USA. [Johnson, Richard; MacCoss, Michael J.] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Karasev, Alexander] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. [Gray, Stewart M.; Cilia, Michelle] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Cilia, M (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM mlc68@cornell.edu FU USDA NIFA [1907-22000-021-20]; Boyce Thompson Institute; NSF [1354309] FX The authors gratefully acknowledge Drs. Todd Greco, Ileana Cristea (Princeton) and an anonymous reviewer for helpful feedback, Keith Rivera at Cold Spring Harbor Laboratory for feedback on methods, James VanEe and John Flaherty at Cornell University's Institute for Biotechnology Bio-IT Facility for IT support, Tom Hammond and Rogerio Santos (Boyce Thompson Institute) for care of plants, Dr. Greg Martin (Boyce Thompson Institute) for access to the N. benthamiana genome sequencing data and Katherine S. Parks and Jaclyn Mahoney (Boyce Thompson Institute) for technical support. Funding was provided by USDA NIFA grant 1907-22000-021-20, the Boyce Thompson Institute, and NSF grant 1354309. NR 114 TC 3 Z9 3 U1 4 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1615-9853 EI 1615-9861 J9 PROTEOMICS JI Proteomics PD JUN PY 2015 VL 15 IS 12 SI SI BP 2098 EP 2112 DI 10.1002/pmic.201400594 PG 15 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CK6NN UT WOS:000356344900014 PM 25787689 ER PT J AU Green, BT Lee, ST Welch, KD Gardner, DR Stegelmeier, BL Davis, TZ AF Green, Benedict T. Lee, Stephen T. Welch, Kevin D. Gardner, Dale R. Stegelmeier, Bryan L. Davis, T. Zane TI The serum concentrations of lupine alkaloids in orally-dosed Holstein cattle SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Lupine; Biomarkers; Anagyrine; Cattle ID CROOKED CALF DISEASE; NICOTINIC ACETYLCHOLINE-RECEPTORS; NORDITERPENOID ALKALOIDS; IDENTIFICATION; LEUCOPHYLLUS; ANAGYRINE; WASHINGTON; BIOMARKERS; RANGELANDS; SELECTION AB Teratogenic alkaloid-containing Lupinus spp. cause congenital defects known as crooked calf disease that is periodically economically devastating for the cattle industry. Previous research indicates that cattle breeds may eliminate plant toxins differently, potentially altering their susceptibility. The objective of this study was to describe the toxicokinetics in Holsteins of anagyrine, the teratogenic lupine alkaloid that produces crooked calf disease. Other alkaloids including lupanine, an unidentified alkaloid and 5,6-dehydrolupanine were also evaluated. Dried ground Lupinus leucophyllus was orally dosed to four Holstein steers and blood samples were collected for 96 h, analyzed for serum alkaloid concentrations and toxicokinetic parameters calculated. The serum elimination of anagyrine in Holstein steers was faster than those reported for beef breeds. This suggests that Holsteins may be less susceptible to lupine-induced crooked calf disease. Additional work is needed to confirm these findings and to verify if there is a breed difference in disease incidence or severity. Published by Elsevier Ltd. C1 [Green, Benedict T.; Lee, Stephen T.; Welch, Kevin D.; Gardner, Dale R.; Stegelmeier, Bryan L.; Davis, T. Zane] ARS, Poisonous Plant Res Lab, USDA, Logan, UT USA. RP Green, BT (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM Ben.Green@ars.usda.gov FU USDA/ARS FX The authors thank Charles Hailes, Edward L. Knoppel, Scott Larsen, Isabelle McCollum, Kermit Price, Rex Probst, and Clint Stonecipher for their assistance. This research was supported by USDA/ARS. NR 38 TC 1 Z9 1 U1 1 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD JUN PY 2015 VL 100 BP 239 EP 244 DI 10.1016/j.rvsc.2015.04.001 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CK9KD UT WOS:000356558100037 PM 25912242 ER PT J AU Sluis, A Hake, S AF Sluis, Aaron Hake, Sarah TI Organogenesis in plants: initiation and elaboration of leaves SO TRENDS IN GENETICS LA English DT Review ID LATERAL ORGAN BOUNDARIES; SHOOT APICAL MERISTEM; RELATIVE CARDAMINE-HIRSUTA; POLAR AUXIN TRANSPORT; BOX PROTEIN TIR1; TRANSCRIPTION FACTOR; LEAF DEVELOPMENT; KNOX GENES; MEDICAGO-TRUNCATULA; REGULATORY NETWORK AB Plant organs initiate from meristems and grow into diverse forms. After initiation, organs enter a morphological phase where they develop their shape, followed by differentiation into mature tissue. Investigations into these processes have revealed numerous factors necessary for proper development, including transcription factors such as the KNOTTED-LIKE HOMEOBOX (KNOX) genes, the hormone auxin, and miRNAs. Importantly, these factors have been shown to play a role in organogenesis in various diverse model species, revealing both deep conservation of regulatory strategies and evolutionary novelties that led to new plant forms. We review here recent work in understanding the regulation of organogenesis and in particular leaf formation, highlighting how regulatory modules are often redeployed in different organ types and stages of development to achieve diverse forms through the balance of growth and differentiation. C1 [Sluis, Aaron] Univ Calif Berkeley, Ctr Plant Gene Express, Albany, CA 94710 USA. USDA ARS, Albany, CA 94710 USA. RP Sluis, A (reprint author), Univ Calif Berkeley, Ctr Plant Gene Express, 800 Buchanan St, Albany, CA 94710 USA. FU NIH [2T32GM007127-36A1]; NIFA [2012-67014-19429] FX The authors thank the reviewers who gave them helpful and constructive comments and Naomi On for a picture of a tomato leaf. A.S. was supported by NIH training grant 2T32GM007127-36A1. The work was supported by NIFA grant 2012-67014-19429. NR 91 TC 13 Z9 13 U1 12 U2 53 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0168-9525 J9 TRENDS GENET JI Trends Genet. PD JUN PY 2015 VL 31 IS 6 BP 300 EP 306 DI 10.1016/j.tig.2015.04.004 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA CL0MI UT WOS:000356636400004 PM 26003219 ER PT J AU Asbjornsen, H Mayer, AS Jones, KW Selfa, T Saenz, L Kolka, RK Halvorsen, KE AF Asbjornsen, Heidi Mayer, Alex S. Jones, Kelly W. Selfa, Theresa Saenz, Leonardo Kolka, Randall K. Halvorsen, Kathleen E. TI Assessing Impacts of Payments for Watershed Services on Sustainability in Coupled Human and Natural Systems SO BIOSCIENCE LA English DT Article DE payments for ecosystem services; water quality and quantity; indicators; monitoring and evaluation; interdisciplinary research ID ECOSYSTEM SERVICES; ENVIRONMENTAL SERVICES; CONCEPTUAL-FRAMEWORK; DESIGNING PAYMENTS; SOCIO-HYDROLOGY; LATIN-AMERICA; RIVER-BASIN; LAND-USE; MANAGEMENT; POVERTY AB Payments for watershed services (PWS) as a policy tool for enhancing water quality and supply have gained momentum in recent years, but their ability to lead to sustainable watershed outcomes is uncertain. Consequently, the demand for effective monitoring and evaluation (M&E) of PWS impacts on coupled human and natural systems (CHANS) and their implications for watershed sustainability (WS) is increasing. The theoretical foundations and practical applications of WS frameworks, which integrate biophysical and socioeconomic indicators to assess progress toward WS goals, have been extensively explored but rarely applied to PWS. We develop the PWS-WS framework as an approach for guiding indicator selection to improve knowledge about the complex drivers, interactions, and feedback between PWS and CHANS. A review of the PWS and WS literatures provides a basis for comparing and contrasting indicators. Using two case studies, we illustrate how applying the PWS-WS framework using a place-based, contextualized approach enhances potential for sustainable watershed outcomes. C1 [Asbjornsen, Heidi] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Asbjornsen, Heidi] Univ New Hampshire, Earth Syst Res Ctr, Inst Earth Oceans & Space, Durham, NH 03824 USA. [Halvorsen, Kathleen E.] Michigan Technol Univ, Dept Social Sci, Houghton, MI 49931 USA. [Halvorsen, Kathleen E.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Mayer, Alex S.] Michigan Technol Univ, Dept Civil & Environm Engn, Houghton, MI 49931 USA. [Jones, Kelly W.] Colorado State Univ, Dept Human Dimens Nat Resources, Ft Collins, CO 80523 USA. [Selfa, Theresa] SUNY Syracuse, Coll Environm Sci & Forestry, Dept Environm Studies, Syracuse, NY 13210 USA. [Saenz, Leonardo] Conservat Int, Washington, DC USA. [Kolka, Randall K.] USDA, No Res Stn, Forest Serv, Grand Rapids, MI USA. RP Asbjornsen, H (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. EM heidi.asbjornsen@unh.edu FU NSF's Dynamics of Coupled Natural-Human Systems (CNH) program [1313804] FX The authors would like to thank Sophie Avila-Foucat, Russ Congalton, and Robert Manson for contributing to early discussions about PWS-CHANS in Mexico. that inspired our thinking for this paper, Mariana Zareth Nava Lopez for assembling and summarizing the literature, and Z. Carter Berry for reviewing and providing valuable feedback on an earlier draft of the manuscript. The authors would like to acknowledge NSF's Dynamics of Coupled Natural-Human Systems (CNH) program grant no. 1313804 for partial funding in support of the research conducted for this synthesis. NR 64 TC 3 Z9 3 U1 8 U2 42 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD JUN PY 2015 VL 65 IS 6 BP 579 EP 591 DI 10.1093/biosci/biv051 PG 13 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CJ8UQ UT WOS:000355779400007 ER PT J AU Filatova, LY Donovan, DM Foster-Frey, J Pugachev, VG Dmitrieva, NF Chubar, TA Klyachko, NL Kabanov, AV AF Filatova, Lyubov Y. Donovan, David M. Foster-Frey, Juli Pugachev, Vladimir G. Dmitrieva, Natalia F. Chubar, Tatiana A. Klyachko, Natalia L. Kabanov, Alexander V. TI Bacteriophage phi11 lysin: Physicochemical characterization and comparison with phage phi80 alpha lysin SO ENZYME AND MICROBIAL TECHNOLOGY LA English DT Article DE Bacteriolytic enzymes; Phages; Staphylococcal infections; Activity; Stability; Structure ID CIRCULAR-DICHROISM SPECTRA; STAPHYLOCOCCUS-AUREUS; ENDOLYSIN; ANTIMICROBIALS; HYDROLASE; CHAP(K) AB Phage lytic enzymes are promising antimicrobial agents. Lysins of phages phi11 (LysPhi11) and phi80 alpha (LysPhi80 alpha) can lyse (destroy) cells of antibiotic-resistant strains of Staphylococcus aureus. Stability of enzymes is one of the parameters making their practical use possible. The objectives of the study were to investigate the stability of lysins of phages phi11 and phi80 alpha in storage and functioning conditions, to identify optimum storage conditions and causes of inactivation. Stability of the recombinant LysPhi11 and LysPhi80 alpha was studied using turbidimetry. CD-spectroscopy, dynamic light scattering, and electrophoresis were used to identify causes of inactivation. At 37 degrees C, pH 7.5 and concentration of NaCl not higher than 150 mM, LysPhi11 molecules contain a high percentage of random coils (43%). However, in spite of this the enzyme has high activity (0.4-0.8 OD600nm s(-1) mg(-1)). In storage conditions (4 degrees C and 22 degrees C, pH 6.0-9.0, 10-500 mM NaCl) LysPhi11 is inactivated by a monomolecular mechanism. The optimum storage conditions for LysPhi11 (4 degrees C, pH 6.0-7.5, 10 mM NaCl) were selected under which the time of the enzyme half-inactivation is 120-160 days. LysPhi80 alpha stability is insufficient: at 37 degrees C the enzyme loses half of its activity almost immediately; at 4 degrees C and 22 degrees C the time of half-inactivation of LysPhi80 alpha varies in the range from several hours to 3 days. Despite the common properties in the manifestation of antistaphylococcal activity the kinetic behavior of the enzymes is different. LysPhi11 is a more promising candidate to be used as an antimicrobial agent. (c) 2015 Elsevier Inc. All rights reserved. C1 [Filatova, Lyubov Y.; Chubar, Tatiana A.; Klyachko, Natalia L.; Kabanov, Alexander V.] Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Enzymol, Moscow 117234, Russia. [Donovan, David M.; Foster-Frey, Juli] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, NEA,USDA, Beltsville, MD USA. [Pugachev, Vladimir G.] FBSE SRC Virol & Bioengn Vector, Novosibirsk, Russia. [Dmitrieva, Natalia F.] IM Sechenov First Moscow State Med Univ, Moscow, Russia. [Klyachko, Natalia L.] Pirogov Russian Natl Res Med Univ RNRMU, Moscow, Russia. [Kabanov, Alexander V.] Univ N Carolina, Div Mol Pharmaceut, Ctr Nanotechnol Drug Delivery, UNC Eshelman Sch Pharm, Chapel Hill, NC USA. RP Filatova, LY (reprint author), Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Enzymol, Moscow 117234, Russia. EM luboff.filatova@gmail.com FU Ministry of Education and Science of the Russian Federation [11.G34.31.0004]; [RFBR-15-04-07995]; [RSF-14-13-00731] FX The study was supported by the Ministry of Education and Science of the Russian Federation (No. 11.G34.31.0004) and RFBR-15-04-07995 and RSF-14-13-00731. NR 24 TC 2 Z9 4 U1 1 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0141-0229 EI 1879-0909 J9 ENZYME MICROB TECH JI Enzyme Microb. Technol. PD JUN PY 2015 VL 73-74 BP 51 EP 58 DI 10.1016/j.enzmictec.2015.03.005 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CK3HZ UT WOS:000356108500007 PM 26002504 ER PT J AU Roman, IS Bartolome, L Gee, WS Alonso, RM Beck, JJ AF Roman, Itxaso San Bartolome, Luis Gee, Wai S. Alonso, Rosa M. Beck, John J. TI Comparison of ex situ volatile emissions from intact and mechanically damaged walnuts SO FOOD RESEARCH INTERNATIONAL LA English DT Article DE Walnut; Volatiles; Damaged; Semiochemicals; HS-SPME ID FEMALE CODLING MOTH; AMYELOIS-TRANSITELLA; NAVEL ORANGEWORM; ELECTROPHYSIOLOGICAL RESPONSES; CHEMICAL-COMPOSITION; PLANT VOLATILES; FUNGAL SPORES; L COLEOPTERA; PISTACHIO; ALMOND AB The codling moth (Cydia pomonella) and navel orangeworm (Amyelois transitella) are insect pests that inflict serious economic damage to California walnuts. Feeding by these larvae causes physical damage to the nut and can lead to contamination by aflatoxigenic fungi. Over the years volatile natural products have played a critical role in efforts to control or monitor these and other insect pest moths. The ex situ volatile emissions from intact and mechanically damaged Howard variety walnuts from the California Central Valley were evaluated over the course of a typical growing season. The volatile profiles were compared and differences in emission considered as a means to identify candidate volatiles for use in host plant-based attractants or in conjunction with pheromone blends to enhance attractancy. Walnut volatiles were extracted by headspace solid phase microextraction (HS-SPME) in a semi-closed system and analyzed by gas chromatography mass spectrometry (GC-MS). Ninety two volatiles were identified, including monoterpenes as the predominant class of compounds. A multivariate analysis of the data highlighted two sampling periods (late July-late August) where intact walnuts and mechanically damaged walnuts can be distinguished due to their volatile profile composition. The results of this study provide relevant information regarding potential host plant-based semiochemicals of two insect pests, valuable data regarding the ambient odors these insects encounter in walnut orchards and add some potentially interesting volatile compounds to the existing literature. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Roman, Itxaso San; Alonso, Rosa M.] Univ Basque Country, Dept Analyt Chem, Fac Sci & Technol, UPV EHU, Bilbao 48080, Spain. [Roman, Itxaso San; Gee, Wai S.; Beck, John J.] ARS, USDA, Foodborne Toxin Detect & Prevent, Western Reg Res Ctr, Albany, CA 94710 USA. [Bartolome, Luis] Univ Basque Country, Fac Sci & Technol, Cent Anal Serv SGIker, UPV EHU, Bilbao 48080, Spain. [Beck, John J.] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA. RP Roman, IS (reprint author), Univ Basque Country, Dept Analyt Chem, Fac Sci & Technol, UPV EHU, POB 644, Bilbao 48080, Spain. OI ALONSO ROJAS, ROSA M./0000-0001-9046-6602 FU Basque Government; University of the Basque Country [IT789-13, UFI11/23]; USDA-ARS CRIS project [5325-42000-037]; University of the Basque Country FX Authors thank the Basque Government and the University of the Basque Country (IT789-13 and UFI11/23) and USDA-ARS CRIS project 5325-42000-037 for financial support, the SGIker technical support (UPV/EHU, MICINN, GV/EJ, ERDF and ESF), and N. Baig for technical assistance and productive discussions. I. San Roman thanks the University of the Basque Country for the pre-doctoral grant. Finally, the authors graciously acknowledge D&D Farms of Yuba City, California for access to, and walnut samples from their walnut orchards. NR 31 TC 0 Z9 0 U1 2 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD JUN PY 2015 VL 72 BP 198 EP 207 DI 10.1016/j.foodres.2015.04.009 PG 10 WC Food Science & Technology SC Food Science & Technology GA CK3NK UT WOS:000356122600025 ER PT J AU Crutcher, FK Moran-Diez, ME Ding, SL Liu, JG Horwitz, BA Mukherjee, PK Kenerley, CM AF Crutcher, Frankie K. Moran-Diez, Maria E. Ding, Shengli Liu, Jinggao Horwitz, Benjamin A. Mukherjee, Prasun K. Kenerley, Charles M. TI A paralog of the proteinaceous elicitor SM1 is involved in colonization of maize roots by Trichoderma Wrens SO FUNGAL BIOLOGY LA English DT Article DE Cerato-platanin; Biocontrol; Induced systemic resistance; Root colonization ID INDUCED SYSTEMIC RESISTANCE; PLATANIN FAMILY PROTEIN; F-SP PLATANI; CERATO-PLATANIN; FUNCTIONAL-CHARACTERIZATION; SYMBIOTIC ASSOCIATION; MAGNAPORTHE-GRISEA; PHYTOTOXIC PROTEIN; VIRENS; EXPRESSION AB The biocontrol agent, Trichoderma uirens, has the ability to protect plants from pathogens by eliciting plant defense responses, involvement in mycoparasitism, or secreting antagonistic secondary metabolites. SM1, an elicitor of induced systemic resistance (ISR), was found to have three paralogs within the T. uirens genome. The paralog sm2 is highly expressed in the presence of plant roots. Gene deletion mutants of sm2 were generated and the mutants were found to overproduce SM1. The ability to elicit ISR in maize against Colletotrichum graminicola was not compromised for the mutants compared to that of wild type isolate. However, the deletion strains had a significantly lowered ability to colonize maize roots. This appears to be the first report on the involvement of an effector-like protein in colonization of roots by Trichoderma. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Crutcher, Frankie K.; Moran-Diez, Maria E.; Ding, Shengli; Mukherjee, Prasun K.; Kenerley, Charles M.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Crutcher, Frankie K.; Liu, Jinggao] ARS, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. [Moran-Diez, Maria E.] Lincoln Univ, Bioprotect Res Ctr, Canterbury 7647, New Zealand. [Horwitz, Benjamin A.] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel. [Mukherjee, Prasun K.] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India. RP Kenerley, CM (reprint author), Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. EM c-kenerley@tamu.edu FU National Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [2008-35319-04470]; Texas Department of Agriculture; U5S-Israel Binational Agricultural Research and Development Fund [TB-8031-08]; USDA National Needs Fellowship FX We would like to acknowledge Dr. Kranthi Mandadi (Texas A&M University) for his expertise on protein solubility and Dr. Tamar Ziv (Smoler Protein Center, Technion - Israel Institute of Technology) for proteomic confirmation of Pichia-expressed SM2. This work was supported by the National Research Initiative Competitive Grants Program (Grant no. 2008-35319-04470) from the USDA National Institute of Food and Agriculture, and the Texas Department of Agriculture and the U5S-Israel Binational Agricultural Research and Development Fund (Grant no. TB-8031-08). Three years of fellowship support were also provided by a USDA National Needs Fellowship. NR 51 TC 1 Z9 2 U1 1 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD JUN PY 2015 VL 119 IS 6 BP 476 EP 486 DI 10.1016/j.funbio.2015.01.004 PG 11 WC Mycology SC Mycology GA CK4JG UT WOS:000356189500003 PM 25986544 ER PT J AU Chettri, P Ehrlich, KC Bradshaw, RE AF Chettri, Pranav Ehrlich, Kenneth C. Bradshaw, Rosie E. TI Regulation of the aflatoxin-like toxin dothistromin by Aflj SO FUNGAL BIOLOGY LA English DT Article DE Gene regulation; Mycotoxin; Polyketide; Secondary metabolite ID GENE-CLUSTER; SECONDARY METABOLISM; ASPERGILLUS-FLAVUS; FOREST PATHOGEN; BIOSYNTHESIS; SEPTOSPORUM; LAEA; VEA AB Biosynthesis by Aspergillus parasiticus of aflatoxin, one of the most potent known naturally occurring Carcinogens, requires the activity of two regulatory proteins, AflR and AflJ, which are encoded by divergently transcribed genes within the aflatoxin gene cluster. Although the Zn(2)Cys(6) transcription factor, AflR, has been well-studied, the role of AflJ as a transcription regulatory factor is not well understood. An AflJ-like gene (DsAflJ) is also present in the genome of the pine needle pathogen Dothistroma septosporum and is similarly divergently transcribed from an AflR orthologue (DsAflR). These genes are involved in biosynthesis of dothistromin, a toxic virulence factor related to aflatoxin. DsAflJ mutants produced low levels of dothistromin (<25-fold less than wild-type); this was in contrast to earlier work with A. parasiticus AflJ mutants in which aflatoxin production was more severely impaired. As expected, complementation of D. septosporum mutants with an intact copy of the DsAflJ gene regained production of wild-type levels of dothistromin, although levels were not further increased by over-expression in multi-copy strains. However, heterologous AflJ genes from Aspergillus spp. were unable to complement DsAflJ mutants, suggesting that the proteins function differently in these species. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Chettri, Pranav; Bradshaw, Rosie E.] Massey Univ, Inst Fundamental Sci, Bioprotect Res Ctr, Palmerston North 4442, New Zealand. [Ehrlich, Kenneth C.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Bradshaw, RE (reprint author), Massey Univ, Inst Fundamental Sci, Manawatu Campus, Palmerston North 4442, New Zealand. EM p.chettri@massey.ac.nz; ehrlich8@gmail.com; r.e.bradshaw@massey.ac.nz RI chettri, pranav/E-4292-2015; Bradshaw, Rosie/C-7846-2011; OI Bradshaw, Rosie/0000-0001-5228-2685; Chettri, Pranav/0000-0001-5464-1274 FU Bio-Protection Research Centre, New Zealand [3-9-MU] FX This work was supported by the Bio-Protection Research Centre, New Zealand, under Grant 3-9-MU. NR 23 TC 1 Z9 1 U1 1 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD JUN PY 2015 VL 119 IS 6 BP 503 EP 508 DI 10.1016/j.funbio.2015.01.007 PG 6 WC Mycology SC Mycology GA CK4JG UT WOS:000356189500006 PM 25986547 ER PT J AU Oliver, AK Brown, SP Callaham, MA Jumpponen, A AF Oliver, Alena K. Brown, Shawn P. Callaham, Mac A., Jr. Jumpponen, Ari TI Polymerase matters: non-proofreading enzymes inflate fungal community richness estimates by up to 15 % SO FUNGAL ECOLOGY LA English DT Article DE Community composition; DNA polymerase; Fungal communities; Proofreading; Richness ID US FOREST ECOSYSTEM; RARE BIOSPHERE; PRIMERS; DIVERSITY; WRINKLES AB Rare taxa overwhelm metabarcoding data generated using next-generation sequencing (NGS). Low frequency Operational Taxonomic Units (OTUs) may be artifacts generated by PCR-amplification errors resulting from polymerase mispairing. We analyzed two Internal Transcribed Spacer 2 (ITS2) MiSeq libraries generated with proofreading (Thermo Scientific Phusion (R)) and non-proofreading (Thermo Scientific Phire (R)) polymerases from the same MiSeq reaction, the same samples, using the same DNA tags, and with two different clustering methods to evaluate the effect of polymerase and clustering tool choices on the estimates of richness, diversity and community composition. Our data show that, while the overall communities are comparable, OTU richness is exaggerated by the use of the non-proofreading polymerase-up to 15 % depending on the clustering method, and on the threshold of low frequency OTU removal. The overestimation of richness also consistently led to underestimation of community evenness, a result of increase in the low frequency OTUs. Stringent thresholds of eliminating the rare reads remedy this issue; exclusion of reads that occurred <= 10 times reduced overestimated OTU numbers to <0.3 %. As a result of these findings, we strongly recommend the use of proofreading polymerases to improve the data integrity as well as the use of stringent culling thresholds for rare sequences to minimize overestimation of community richness. (C) 2015 Elsevier Ltd and The British Mycological Society. All rights reserved. C1 [Oliver, Alena K.; Brown, Shawn P.; Jumpponen, Ari] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Callaham, Mac A., Jr.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Jumpponen, A (reprint author), Kansas State Univ, Div Biol, 433 Ackert Hall, Manhattan, KS 66506 USA. EM ari@ksu.edu FU USDA Forest Service Cooperative Agreement [11-CA-11330136-126]; DOE [DE-SC0004953]; National Science Foundation [NSF DGE-0841414] FX This work was supported by USDA Forest Service Cooperative Agreement 11-CA-11330136-126 to A.J. We would, like to thank Evelyn Wenk for coordinating the sampling. Alina Akhunova and Huang Liang at the Integrated Genomics Facility at Kansas State University performed the sequencing. The use of trade names in this contribution does not constitute official endorsement of these products by USDA or any of its subsidiary agencies. Alena Oliver and Ari Jumpponen were partly supported by DOE Contract No. DE-SC0004953. Shawn Brown was supported with a National Science Foundation GK-12 fellowship (NSF DGE-0841414, PI Ferguson). This is contribution no. 15-340-J from the Kansas Agricultural Experiment Station. NR 19 TC 8 Z9 8 U1 1 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1754-5048 EI 1878-0083 J9 FUNGAL ECOL JI Fungal Ecol. PD JUN PY 2015 VL 15 BP 86 EP 89 DI 10.1016/j.funeco.2015.03.003 PG 4 WC Ecology; Mycology SC Environmental Sciences & Ecology; Mycology GA CK3IJ UT WOS:000356109500010 ER PT J AU Hulse-Kemp, AM Ashrafi, H Stoffel, K Zheng, XT Saski, CA Scheffler, BE Fang, DD Chen, ZJ Van Deynze, A Stelly, DM AF Hulse-Kemp, Amanda M. Ashrafi, Hamid Stoffel, Kevin Zheng, Xiuting Saski, Christopher A. Scheffler, Brian E. Fang, David D. Chen, Z. Jeffrey Van Deynze, Allen Stelly, David M. TI BAC-End Sequence-Based SNP Mining in Allotetraploid Cotton (Gossypium) Utilizing Resequencing Data, Phylogenetic Inferences, and Perspectives for Genetic Mapping SO G3-GENES GENOMES GENETICS LA English DT Article DE SNP genotyping; cotton genomics; resequencing; BAC-derived SNPs; intraspecific ID HIRSUTUM L.; LINKAGE DISEQUILIBRIUM; GENOME SEQUENCE; DIVERSITY; MAP; EVOLUTION; HYBRID; GENUS; TOOL AB A bacterial artificial chromosome library and BAC-end sequences for cultivated cotton (Gossypium hirsutum L.) have recently been developed. This report presents genome-wide single nucleotide polymorphism (SNP) mining utilizing resequencing data with BAC-end sequences as a reference by alignment of 12 G. hirsutum L. lines, one G. barbadense L. line, and one G. longicalyx Hutch and Lee line. A total of 132,262 intraspecific SNPs have been developed for G. hirsutum, whereas 223,138 and 470,631 interspecific SNPs have been developed for G. barbadense and G. longicalyx, respectively. Using a set of interspecific SNPs, 11 randomly selected and 77 SNPs that are putatively associated with the homeologous chromosome pair 12 and 26, we mapped 77 SNPs into two linkage groups representing these chromosomes, spanning a total of 236.2 cMin an interspecific F2 population (G. barbadense 3-79 x G. hirsutum TM-1). The mapping results validated the approach for reliably producing large numbers of both intraspecific and interspecific SNPs aligned to BAC-ends. This will allow for future construction of high-density integrated physical and genetic maps for cotton and other complex polyploid genomes. The methods developed will allow for future Gossypium resequencing data to be automatically genotyped for identified SNPs along the BAC-end sequence reference for anchoring sequence assemblies and comparative studies. C1 [Hulse-Kemp, Amanda M.; Zheng, Xiuting; Stelly, David M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Hulse-Kemp, Amanda M.; Stelly, David M.] Texas A&M Univ, Interdisciplinary Genet Program, College Stn, TX 77843 USA. [Ashrafi, Hamid; Stoffel, Kevin; Van Deynze, Allen] Univ Calif Davis, Seed Biotechnol Ctr, Davis, CA 95616 USA. [Saski, Christopher A.] Clemson Univ, Genom Inst, Clemson, SC 29634 USA. [Scheffler, Brian E.] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Fang, David D.] USDA ARS, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. [Chen, Z. Jeffrey] Univ Texas Austin, Ctr Computat Biol & Bioinformat, Dept Mol Biosci, Austin, TX 78712 USA. [Chen, Z. Jeffrey] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA. RP Stelly, DM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, MS 2474,370 Olsen Blvd, College Stn, TX 77843 USA. EM stelly@tamu.edu OI Scheffler, Brian/0000-0003-1968-8952 FU Cotton Incorporated [03-430]; National Science Foundation, Plant Genome Research Program (NSF-PGRP) [IOS1025947] FX The authors acknowledge the Whole Systems Genomics Initiative (WSGI) for providing computational resources and systems administration support for the WSGI HPC cluster, and Kranti Konganti for assistance with utilizing the WSGI resources. The authors thank Fei Wang for maintaining the AgriGenomics Laboratory where KASP assays were performed, Alan Pepper (TAMU) for providing DNA for TX0231, and Iain Wilson's group (CSIRO) for providing DNA for Australian and Indian lines included in G. hirsutum screening. This work was supported by funding from Cotton Incorporated grant no. 03-430 and the National Science Foundation, Plant Genome Research Program (NSF-PGRP) grant no. IOS1025947. NR 37 TC 2 Z9 3 U1 2 U2 13 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN 1 PY 2015 VL 5 IS 6 BP 1095 EP 1105 DI 10.1534/g3.115.017749 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA CK4FC UT WOS:000356176000009 PM 25858960 ER PT J AU Hulse-Kemp, AM Lemm, J Plieske, J Ashrafi, H Buyyarapu, R Fang, DD Frelichowski, J Giband, M Hague, S Hinze, LL Kochan, KJ Riggs, PK Scheffler, JA Udall, JA Ulloa, M Wang, SS Zhu, QH Bag, SK Bhardwaj, A Burke, JJ Byers, RL Claverie, M Gore, MA Harker, DB Islam, MS Jenkins, JN Jones, DC Lacape, JM Llewellyn, DJ Percy, RG Pepper, AE Poland, JA Rai, KM Sawant, SV Singh, SK Spriggs, A Taylor, JM Wang, F Yourstone, SM Zheng, XT Lawley, CT Ganal, MW Van Deynze, A Wilson, IW Stelly, DM AF Hulse-Kemp, Amanda M. Lemm, Jana Plieske, Joerg Ashrafi, Hamid Buyyarapu, Ramesh Fang, David D. Frelichowski, James Giband, Marc Hague, Steve Hinze, Lori L. Kochan, Kelli J. Riggs, Penny K. Scheffler, Jodi A. Udall, Joshua A. Ulloa, Mauricio Wang, Shirley S. Zhu, Qian-Hao Bag, Sumit K. Bhardwaj, Archana Burke, John J. Byers, Robert L. Claverie, Michel Gore, Michael A. Harker, David B. Islam, Md S. Jenkins, Johnie N. Jones, Don C. Lacape, Jean-Marc Llewellyn, Danny J. Percy, Richard G. Pepper, Alan E. Poland, Jesse A. Rai, Krishan Mohan Sawant, Samir V. Singh, Sunil Kumar Spriggs, Andrew Taylor, Jen M. Wang, Fei Yourstone, Scott M. Zheng, Xiuting Lawley, Cindy T. Ganal, Martin W. Van Deynze, Allen Wilson, Iain W. Stelly, David M. TI Development of a 63K SNP Array for Cotton and High-Density Mapping of Intraspecific and Interspecific Populations of Gossypium spp. SO G3-GENES GENOMES GENETICS LA English DT Article DE linkage analysis; recombination; interspecific SNPs; intraspecific SNPs; breeding ID HIRSUTUM L.; GENETIC DIVERSITY; UPLAND COTTON; MAP; GENOME; EVOLUTION; SEQUENCE; IDENTIFICATION; COLLECTION; GERMPLASM AB High-throughput genotyping arrays provide a standardized resource for plant breeding communities that are useful for a breadth of applications including high-density genetic mapping, genome-wide association studies (GWAS), genomic selection (GS), complex trait dissection, and studying patterns of genomic diversity among cultivars and wild accessions. We have developed the CottonSNP63K, an Illumina Infinium array containing assays for 45,104 putative intraspecific single nucleotide polymorphism (SNP) markers for use within the cultivated cotton species Gossypium hirsutum L. and 17,954 putative interspecific SNP markers for use with crosses of other cotton species with G. hirsutum. The SNPs on the array were developed from 13 different discovery sets that represent a diverse range of G. hirsutum germplasm and five other species: G. barbadense L., G. tomentosum Nuttal x Seemann, G. mustelinum Miers x Watt, G. armourianum Kearny, and G. longicalyx J.B. Hutchinson and Lee. The array was validated with 1,156 samples to generate cluster positions to facilitate automated analysis of 38,822 polymorphic markers. Two high-density genetic maps containing a total of 22,829 SNPs were generated for two F-2 mapping populations, one intraspecific and one interspecific, and 3,533 SNP markers were co-occurring in both maps. The produced intraspecific genetic map is the first saturated map that associates into 26 linkage groups corresponding to the number of cotton chromosomes for a cross between two G. hirsutum lines. The linkage maps were shown to have high levels of collinearity to the JGI G. raimondii Ulbrich reference genome sequence. The CottonSNP63K array, cluster file and associated marker sequences constitute a major new resource for the global cotton research community. C1 [Hulse-Kemp, Amanda M.; Hague, Steve; Wang, Fei; Zheng, Xiuting; Stelly, David M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Riggs, Penny K.; Pepper, Alan E.; Stelly, David M.] Texas A&M Univ, Interdisciplinary Degree Program Genet, College Stn, TX 77843 USA. [Lemm, Jana; Plieske, Joerg; Ganal, Martin W.] TraitGenet GmbH, D-06466 Gatersleben, Germany. [Ashrafi, Hamid; Van Deynze, Allen] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Ashrafi, Hamid; Van Deynze, Allen] Univ Calif Davis, Seed Biotechnol Ctr, Davis, CA 95616 USA. [Buyyarapu, Ramesh] Dow AgroSci, Trait Genet & Technol, Indianapolis, IN 46268 USA. [Fang, David D.; Islam, Md S.] USDA ARS SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. [Frelichowski, James; Hinze, Lori L.; Wang, Shirley S.; Percy, Richard G.] USDA ARS SRRC, Crop Germplasm Res Unit, College Stn, TX 77843 USA. [Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Giband, Marc; Claverie, Michel; Lacape, Jean-Marc] CIRAD, UMR AGAP, F-34398 Montpellier, France. [Giband, Marc] EMBRAPA, Algodao, Nucleo Cerrado, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Kochan, Kelli J.; Riggs, Penny K.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Scheffler, Jodi A.] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. [Udall, Joshua A.; Byers, Robert L.; Harker, David B.; Yourstone, Scott M.] Brigham Young Univ, Plant & Wildlife Sci Dept, Provo, UT 84602 USA. [Ulloa, Mauricio; Burke, John J.] USDA ARS, PA, Plant Stress & Germplasm Dev Res Unit, Lubbock, TX 79415 USA. [Zhu, Qian-Hao; Llewellyn, Danny J.; Spriggs, Andrew; Taylor, Jen M.; Wilson, Iain W.] CSIRO Agr Flagship, Black Mt Labs, Canberra, ACT 2601, Australia. [Bag, Sumit K.; Bhardwaj, Archana; Rai, Krishan Mohan; Sawant, Samir V.; Singh, Sunil Kumar] CSIR Natl Bot Res Inst, Plant Mol Biol Div, Lucknow 226001, Uttar Pradesh, India. [Gore, Michael A.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Jenkins, Johnie N.] USDA ARS, Genet & Precis Agr Res, Mississippi State, MS 39762 USA. [Jones, Don C.] Cotton Inc, Agr Res, Cary, NC 27513 USA. [Poland, Jesse A.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Manhattan, KS 66506 USA. [Poland, Jesse A.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Lawley, Cindy T.] Illumina Inc, San Francisco, CA 94158 USA. RP Stelly, DM (reprint author), Texas A&M Univ, 370 Olsen Blvd, College Stn, TX 77843 USA. EM stelly@tamu.edu RI Zhu, Qianhao/A-1298-2010; Riggs, Penny/A-8192-2008; Bag, Sumit/B-4010-2013; Llewellyn, Danny/D-5230-2009; Taylor, Jennifer/A-1686-2010; Lacape, Jean-Marc/B-4326-2008; Wilson, Iain/D-4839-2009; OI Riggs, Penny/0000-0003-3296-320X; Bag, Sumit/0000-0001-8907-2841; Poland, Jesse/0000-0002-7856-1399; Hinze, Lori L/0000-0003-3356-1245; Llewellyn, Danny/0000-0001-9535-8707; Taylor, Jennifer/0000-0003-4231-0181; Wilson, Iain/0000-0002-4626-8059; Singh, Sunil Kumar/0000-0002-4897-8812; Buyyarapu, Ramesh/0000-0002-5343-9960 FU National Science Foundation, Plant Genome Research Program (NSF-PGRP) [IOS1025947]; Cotton Incorporated [03-430, 13-326, 08-386, 13-694] FX We thank Alexander Kozik for assistance with the CheckMatrix software, Kranti Konganti for implementing and troubleshooting the CheckMatrix software on the Texas Institute for Genome Sciences and Society HPC cluster, and Jing Yu for incorporation of the SNP data file into the CottonGen database. The authors thank Illumina, Inc. for contributing to expanding the scope of the study to allow for a broader impact across global cotton germplasm. This project was supported by National Science Foundation, Plant Genome Research Program (NSF-PGRP) grant No. IOS1025947, and Cotton Incorporated grants Nos. 03-430, 13-326, 08-386, and 13-694. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The U.S. Department of Agriculture and Texas A&M University System are equal opportunity providers and employers. J.L., J.P., and M.W.G. have competing commercial interests as employees of TraitGenetics, which is a commercial company that performs molecular marker analysis services with this array. C.T.L. is an employee of Illumina, Inc. that manufactures the array. This does not alter the authors' adherence to all of the G3 policies on sharing data and materials. There are no patents or products in development to declare. All remaining authors declare that they have no competing interests. NR 47 TC 20 Z9 23 U1 7 U2 31 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN 1 PY 2015 VL 5 IS 6 BP 1187 EP 1209 DI 10.1534/g3.115.018416 PG 23 WC Genetics & Heredity SC Genetics & Heredity GA CK4FC UT WOS:000356176000017 PM 25908569 ER PT J AU Del Valle-Echevarria, AR Kielkowska, A Bartoszewski, G Havey, MJ AF Del Valle-Echevarria, Angel R. Kielkowska, Agnieszka Bartoszewski, Grzegorz Havey, Michael J. TI The Mosaic Mutants of Cucumber: A Method to Produce Knock-Downs of Mitochondrial Transcripts SO G3-GENES GENOMES GENETICS LA English DT Article DE plant mitochondria; mitochondrial mutant; mitochondrial transcript; Cucumis sativus ID COMPLETE NUCLEOTIDE-SEQUENCE; OXYGEN SPECIES HOMEOSTASIS; RIBOSOMAL-PROTEIN S7; PLANT-MITOCHONDRIA; RESPIRATORY-CHAIN; ESCHERICHIA-COLI; COMPLEX I; ARABIDOPSIS MITOCHONDRIA; ALTERNATIVE OXIDASE; REPETITIVE DNAS AB Cytoplasmic effects on plant performance are well-documented and result from the intimate interaction between organellar and nuclear gene products. In plants, deletions, mutations, or chimerism of mitochondrial genes are often associated with deleterious phenotypes, as well as economically important traits such as cytoplasmic male sterility used to produce hybrid seed. Presently, genetic analyses of mitochondrial function and nuclear interactions are limited because there is no method to efficiently produce mitochondrial mutants. Cucumber (Cucumis sativus L.) possesses unique attributes useful for organellar genetics, including differential transmission of the three plant genomes (maternal for plastid, paternal for mitochondrial, and biparental for nuclear), a relatively large mitochondrial DNA in which recombination among repetitive motifs produces rearrangements, and the existence of strongly mosaic (MSC) paternally transmitted phenotypes that appear after passage of wild-type plants through cell cultures and possess unique rearrangements in the mitochondrial DNA. We sequenced the mitochondrial DNA from three independently produced MSC lines and revealed under-represented regions and reduced transcription of mitochondrial genes carried in these regions relative to the wild-type parental line. Mass spectrometry and Western blots did not corroborate transcriptional differences in the mitochondrial proteome of the MSC mutant lines, indicating that post-transcriptional events, such as protein longevity, may compensate for reduced transcription in MSC mitochondria. Our results support cucumber as a model system to produce transcriptional "knock-downs" of mitochondrial genes useful to study mitochondrial responses and nuclear interactions important for plant performance. C1 [Del Valle-Echevarria, Angel R.; Havey, Michael J.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Havey, Michael J.] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA. [Kielkowska, Agnieszka] Agr Univ Krakow, Fac Hort, PL-31425 Krakow, Poland. [Bartoszewski, Grzegorz] Warsaw Univ Life Sci, Fac Hort Biotechnol & Landscape Architecture, Dept Plant Genet Breeding & Biotechnol, PL-02776 Warsaw, Poland. RP Havey, MJ (reprint author), Univ Wisconsin, USDA ARS, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM mjhavey@wisc.edu RI Bartoszewski, Grzegorz/M-8458-2016 OI Bartoszewski, Grzegorz/0000-0002-6197-770X FU Agricultural Research Service of the United States Department of Agriculture; Graduate School of the University of Wisconsin-Madison; National Institutes of Health (NIH) Biotechnology Predoctoral Training Program (BTP) of the University of Wisconsin-Madison (NIH) [5 T32 GM08349] FX We thank Dr. Christine Chase (University of Florida) for providing ATP4 and PORIN antibodies, Mr. Joseph Walker and Dr. Richard D. Vierstra (UW-Madison) for their help with Western blots, and Dr. Douglas Senalik (USDA-ARS and UW-Madison) for his assistance with the development of coverage graphs. We especially thank Dr. Stefan Malepszy (Warsaw University of the Life Sciences) for his career-long commitment to research on cucumber cell cultures and development of the MSC lines. This research was supported by the Agricultural Research Service of the United States Department of Agriculture, a Science and Medicine Graduate Research Scholars Fellowship, an Advance Opportunity Fellowship of the Graduate School of the University of Wisconsin-Madison, and the National Institutes of Health (NIH) Biotechnology Predoctoral Training Program (BTP) of the University of Wisconsin-Madison (NIH grant 5 T32 GM08349). Names are necessary to report factually on available data; however, the U.S. Department of Agriculture (USDA) neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 81 TC 1 Z9 1 U1 2 U2 7 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN 1 PY 2015 VL 5 IS 6 BP 1211 EP 1221 DI 10.1534/g3.115.017053 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA CK4FC UT WOS:000356176000018 PM 25873637 ER PT J AU Lone, AG Atci, E Renslow, R Beyenal, H Noh, S Fransson, B Abu-Lail, N Park, JJ Gang, DR Call, DR AF Lone, Abdul G. Atci, Erhan Renslow, Ryan Beyenal, Haluk Noh, Susan Fransson, Boel Abu-Lail, Nehal Park, Jeong-Jin Gang, David R. Call, Douglas R. TI Staphylococcus aureus Induces Hypoxia and Cellular Damage in Porcine Dermal Explants SO INFECTION AND IMMUNITY LA English DT Article ID INDUCED APOPTOSIS; CHRONIC WOUNDS; LACTATE-DEHYDROGENASE; CELLS; OXYGEN; P53; PH; INFECTIONS; PROTEIN; SKIN AB We developed a porcine dermal explant model to determine the extent to which Staphylococcus aureus biofilm communities deplete oxygen, change pH, and produce damage in underlying tissue. Microelectrode measurements demonstrated that dissolved oxygen (DO) in biofilm-free dermal tissue was 4.45 +/- 1.17 mg/liter, while DO levels for biofilm-infected tissue declined sharply from the surface, with no measurable oxygen detectable in the underlying dermal tissue. Magnetic resonance imaging demonstrated that biofilm-free dermal tissue had a significantly lower relative effective diffusion coefficient (0.26 +/- 0.09 to 0.30 +/- 0.12) than biofilm-infected dermal tissue (0.40 +/- 0.12 to 0.48 +/- 0.12; P < 0.0001). Thus, the difference in DO level was attributable to biofilm-induced oxygen demand rather than changes in oxygen diffusivity. Microelectrode measures showed that pH within biofilm-infected explants was more alkaline than in biofilm-free explants (8.0 +/- 0.17 versus 7.5 +/- 0.15, respectively; P < 0.002). Cellular and nuclear details were lost in the infected explants, consistent with cell death. Quantitative label-free shotgun proteomics demonstrated that both proapoptotic programmed cell death protein 5 and antiapoptotic macrophage migration inhibitory factor accumulated in the infected-explant spent medium, compared with uninfected-explant spent media (1,351-fold and 58-fold, respectively), consistent with the cooccurrence of apoptosis and necrosis in the explants. Biofilm-origin proteins reflected an extracellular matrix-adapted lifestyle of S. aureus. S. aureus biofilms deplete oxygen, increase pH, and induce cell death, all factors that contribute to impede wound healing. C1 [Lone, Abdul G.; Call, Douglas R.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Atci, Erhan; Beyenal, Haluk; Abu-Lail, Nehal] Washington State Univ, Sch Chem Engn & Bioengn, Pullman, WA 99164 USA. [Renslow, Ryan] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA. [Noh, Susan] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Noh, Susan] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Fransson, Boel; Park, Jeong-Jin; Gang, David R.] Washington State Univ, Dept Vet Clin Sci, Inst Biol Chem, Pullman, WA 99164 USA. RP Call, DR (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM drcall@wsu.edu OI Call, Douglas/0000-0001-6791-055X FU U.S. Department of Defense [DM110308]; Agricultural Animal Health Program, Washington State University; Washington State Agricultural Research Center, Pullman, WA; National Science Foundation [DBI-1229749]; Department of Energy's Office of Biological and Environmental Research at Pacific Northwest National Laboratory; Linus Pauling Distinguished Postdoctoral Fellowship at Pacific Northwest National Laboratory FX This research was supported in part by a grant (DM110308) from the U.S. Department of Defense and by the Agricultural Animal Health Program, Washington State University, and the Washington State Agricultural Research Center, Pullman, WA. Mass spectrometric analysis was performed on an instrument acquired through a Major Research Instrumentation grant (DBI-1229749) from National Science Foundation to DRG. All NMR experiments were performed at the Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility sponsored by the Department of Energy's Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory. Ryan Renslow was also partially supported by a Linus Pauling Distinguished Postdoctoral Fellowship at Pacific Northwest National Laboratory. NR 77 TC 7 Z9 7 U1 0 U2 15 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD JUN PY 2015 VL 83 IS 6 BP 2531 EP 2541 DI 10.1128/IAI.03075-14 PG 11 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CK5DS UT WOS:000356243000030 PM 25847960 ER PT J AU Kormos, PR McNamara, JP Seyfried, MS Marshall, HP Marks, D Flores, AN AF Kormos, Patrick R. McNamara, James P. Seyfried, Mark S. Marshall, Hans Peter Marks, Danny Flores, Alejandro N. TI Bedrock infiltration estimates from a catchment water storage-based modeling approach in the rain snow transition zone SO JOURNAL OF HYDROLOGY LA English DT Article DE Bedrock infiltration; Deep percolation; Mountain block recharge; Rain snow transition zone; Catchment storage; Soil capacitance ID WESTERN UNITED-STATES; LEAF-AREA INDEX; SOIL-WATER; GROUNDWATER RECHARGE; ENERGY-BALANCE; MOUNTAIN BASIN; STREAMFLOW GENERATION; PRECIPITATION PHASE; RUNOFF GENERATION; DEEP-PERCOLATION AB Estimates of bedrock infiltration from mountain catchments in the western U.S. are essential to water resource managers because they provide an estimate of mountain block recharge to regional aquifers. On smaller scales, bedrock infiltration is an important term in water mass balance studies, which attempt to estimate hydrologic states and fluxes in watersheds with fractured or transmissive bedrock. We estimate the a daily time series of bedrock infiltration in a small catchment in the rain snow transition zone in southwest Idaho, using the difference between measured stream discharge and modeled soil drainage. The accuracy of spatial patterns in soil water storage are optimized, rather than the more common approach of minimizing error in integrated quantities such as streamflow. Bedrock infiltration is estimated to be 289 mm 50 mm for the 2011 water year, which is 34% +/- 12% of the precipitation (95% confidence). Soils on the southwest facing slope drain more often throughout the snow season, but the northeast facing slope contributes more total soil drainage for the water year. Peaks in catchment soil drainage and bedrock infiltration coincide with rain on snow events. Published by Elsevier B.V. C1 [Kormos, Patrick R.; McNamara, James P.; Marshall, Hans Peter; Flores, Alejandro N.] Boise State Univ, Dept Geosci, Boise, ID 83725 USA. [Kormos, Patrick R.; Seyfried, Mark S.; Marks, Danny] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. [Kormos, Patrick R.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Kormos, Patrick R.] US Agr Res Serv, Northwest Watershed Res Ctr, Boise, ID 83712 USA. RP Kormos, PR (reprint author), Boise State Univ, Dept Geosci, 1910 Univ Dr, Boise, ID 83725 USA. EM patrick.kormos@ars.usda.gov RI McNamara, James/F-1993-2011; OI Kormos, Patrick/0000-0003-1874-9215 FU Northwest Watershed Research Center; Boise State University Department of Geosciences, Student Research Initiative, and Graduate College; NASA EPSCoR [NNX10AN30A]; Inland Northwest Research Alliance (INRA); NSF-CBET [0854553, 08522]; USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West [5362-13610-008-00D]; USDA-NRCS Water and Climate Center-Portland, Oregon [5362-13610-008-03R]; NSF-EPS [0919514]; NSF EPSCoR [1329513]; NOAA [NA08NWS4620047] FX We thank Jason Williams and Seth Wenger for assisting in the preparation of this manuscript and Pam Aishlin for field data collection and processing. We thank the students, faculty, and scientists at the Agricultural Research Service, Northwest Watershed Research Center, Forest Service, Rocky Mountain Research Station, Boise Aquatics Science Laboratory, and Boise State University Department of Geosciences for intellectual support. We thank the Northwest Watershed Research Center and Boise State University Department of Geosciences, Student Research Initiative, and Graduate College for funding support, travel support, and general support. NASA EPSCoR and Inland Northwest Research Alliance (INRA) provided funding for this project. The collection and processing of the data presented in this paper were funded in part by NSF-CBET (0854553, 08522), USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West (5362-13610-008-00D), USDA-NRCS Water and Climate Center-Portland, Oregon (5362-13610-008-03R), NSF-EPS (0919514), NSF EPSCoR (1329513), NASA EPSCoR award NNX10AN30A, and NOAA (NA08NWS4620047). Any reference to specific equipment types or manufacturers is for information purposes and does not represent a product endorsement or recommendation. Boise State University and the USDA are equal opportunity employers. NR 117 TC 0 Z9 0 U1 3 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD JUN PY 2015 VL 525 BP 231 EP 248 DI 10.1016/j.jhydrol.2015.03.032 PG 18 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CK0HF UT WOS:000355885600019 ER PT J AU Kennedy, CD AF Kennedy, Casey D. TI Hydrologic and nutrient response of groundwater to flooding of cranberry farms in southeastern Massachusetts, USA SO JOURNAL OF HYDROLOGY LA English DT Article DE Groundwater; Floods; Nitrogen; Cranberry; Agriculture ID WATER; EVAPOTRANSPIRATION; MANAGEMENT; WETLANDS; STREAMS; BALANCE; BOG AB Seasonal flooding of cranberry farms is essential for commercial production of cranberries in southeastern Massachusetts, with close to 90% of growers using a flood for harvesting and winter protection. Although periodic flooding results in increased groundwater recharge, it may also exacerbate subsurface transport of dissolved forms of nitrogen and phosphorus. Given the paucity of information on groundwater exchange with cranberry floodwaters, hydrometric measurements were used to solve for the residual term of groundwater recharge in water budgets for three cranberry farms during the harvest and winter floods. Combined with continuous monitoring of water-table depth and discrete sampling of groundwater for analysis of nitrate (NO3-), ammonium (NH4+), and total dissolved phosphorus (TDP), values of groundwater recharge were used to evaluate the hydrologic and nutrient response of groundwater to flooding of cranberry farms. Mean values of groundwater recharge were 11 (+/- 6) and 47 (+/- 11) cm for the harvest and winter floods, respectively (one standard deviation in parentheses). The factor-of-four difference in ground recharge was related to flood holding times that, on average, were twenty days longer for the winter flood. The total estimated seasonal groundwater recharge of 58 cm was about four times higher than that assigned to cranberry farms in regional groundwater flow models. During the floods, 10 to 20-cm increases in water-table depth were observed for wells within 10 m of the farm, contrasting with decreases (or minimal variation) in water-table depth for wells located 100 m or farther from the farm. These spatial patterns in the hydrologic response of groundwater suggested a zone of influence of approximately 100 m from the flooded edge of the farm. Analysis of 43 groundwater samples collected from 10 wells indicated generally low concentrations of TDP in groundwater (<0.32 mu M for 84% of the samples). Nitrate accounted for 85% of the dissolved inorganic N in groundwater, exhibiting a spatial pattern of decreasing concentration with increasing distance from the farm (e.g., values were consistently less than 3.6 pM for wells located similar to 100 m from the flooded edge of farms). For one groundwater well located in proximity to the farm (similar to 10 m), decreases in NO3- concentration from 565 mu M (pre-flood) to 99 mu M (post-flood) were consistent with winter floodwater as a source of low-NO3- groundwater recharge. Published by Elsevier B.V. C1 USDA ARS, Pasture Syst & Watershed Management Res Unit, East Wareham, MA 02538 USA. RP Kennedy, CD (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, East Wareham, MA 02538 USA. EM casey.kennedy@ars.usda.gov NR 48 TC 2 Z9 2 U1 7 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD JUN PY 2015 VL 525 BP 441 EP 449 DI 10.1016/j.jhydrol.2015.02.038 PG 9 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CK0HF UT WOS:000355885600036 ER PT J AU Russell, ML Vermeire, LT Ganguli, AC Hendrickson, JR AF Russell, Morgan L. Vermeire, Lance T. Ganguli, Amy C. Hendrickson, John R. TI Season of fire manipulates bud bank dynamics in northern mixed-grass prairie SO PLANT ECOLOGY LA English DT Article DE Axillary bud; Dormancy; Grassland; Meristem limitation; Prescribed burn; Tiller ID TALLGRASS PRAIRIE; AXILLARY BUDS; GREAT-PLAINS; MERISTEM LIMITATION; SUMMER FIRE; PRECIPITATION; DORMANCY; DROUGHT; PEA; PRODUCTIVITY AB In perennial grassland dominated systems, belowground bud banks regulate plant community dynamics. Plant community responses to disturbance are largely driven by the ability to generate future aboveground growth originating from belowground axillary buds. This study examined bud bank dynamics for Bouteloua gracilis, Hesperostipa comata, and Pascopyrum smithii following fire in northwestern mixed-grass prairie in eastern Montana, USA. Belowground axillary buds were counted and classified for three growing seasons to determine immediate and short-term effects of summer, fall, and spring prescribed burns on patterns of bud bank activity, dormancy, and mortality. Prescribed burns did not result in immediate mortality of B. gracilis, H. comata, or P. smithii buds. Surprisingly, spring prescribed burns immediately increased the number of active B. gracilis buds. Summer fire, however, reduced B. gracilis active bud numbers. Fall burns immediately activated P. smithii buds, whereas fire did not influence any immediate bud dynamics for H. comata. Reduced bud numbers of H. comata may limit the ability to respond to fire. Season of fire directly manipulated bud activity, dormancy, and mortality for these species throughout the growing and dormant seasons following fire. Using season of fire to manipulate bud bank dynamics illustrates potential to improve post-fire management strategies based on known bud development trajectories and bud dynamics following fire. C1 [Russell, Morgan L.] Texas A&M AgriLife Extens Serv, San Angelo, TX 76901 USA. [Vermeire, Lance T.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Ganguli, Amy C.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. [Hendrickson, John R.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Russell, ML (reprint author), Texas A&M AgriLife Extens Serv, 7887 US Highway 87 North, San Angelo, TX 76901 USA. EM morgan.russell@ag.tamu.edu RI Ganguli, Amy/J-3342-2014 OI Ganguli, Amy/0000-0003-3960-1404 NR 47 TC 2 Z9 2 U1 4 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD JUN PY 2015 VL 216 IS 6 BP 835 EP 846 DI 10.1007/s11258-015-0471-y PG 12 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CK4ED UT WOS:000356173000006 ER PT J AU Franklin, J Ripplinger, J Freid, EH Marcano-Vega, H Steadman, DW AF Franklin, Janet Ripplinger, Julie Freid, Ethan H. Marcano-Vega, Humfredo Steadman, David W. TI Regional variation in Caribbean dry forest tree species composition SO PLANT ECOLOGY LA English DT Article DE Bahamas; Bahamian archipelago; Broadleaf subtropical evergreen forest; Forest community; Greater Antilles; Novel ecosystems; Tropical seasonal evergreen forest; Vegetation classification; West Indies ID PUERTO-RICO; RELATIVE ABUNDANCE; LIMESTONE FORESTS; VIRGIN-ISLANDS; SOUTH FLORIDA; CONSERVATION; BAHAMAS; ORDINATION; HABITAT; PLANTS AB How does tree species composition vary in relation to geographical and environmental gradients in a globally rare tropical/subtropical broadleaf dry forest community in the Caribbean? We analyzed data from 153 Forest Inventory and Analysis (FIA) plots from Puerto Rico and the U.S. Virgin Islands (USVI), along with 42 plots that we sampled in the Bahamian Archipelago (on Abaco and Eleuthera Islands). FIA data were collected using published protocols. In the Bahamian Archipelago, we recorded terrain and landscape variables, and identified to species and measured the diameter of all stems a parts per thousand yen5 cm at 1.3 m height in 10 m radius plots. All data were analyzed using clustering, ordination, and indicator species analysis at regional and local scales. Regionally, the largest cluster group included over half of all plots and comprised plots from all three island groups. Indicator species were native Bursera simaruba (Burseraceae) and Metopium toxiferum (Anacardiaceae). Species composition was similar to dry forests throughout the region based on published studies. Other groups we identified at the regional scale consisted of many Puerto Rico and USVI plots that were dominated by non-native species, documenting the widespread nature of novel ecosystems. At the local scale the Bahamian data clustered into two main groups corresponding largely to the two islands sampled, a pattern consistent with the latitudinal aridity gradient. Bahamian dry forests share previously undocumented compositional similarity with native-dominated dry forests found throughout the Caribbean, but they lack extensive post-disturbance novel dry forests dominated by non-native trees found in the Greater Antilles. C1 [Franklin, Janet] Arizona State Univ, Sch Geog Sci & Urban Planning, Tempe, AZ 85287 USA. [Ripplinger, Julie] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Freid, Ethan H.] Bahamas Natl Trust, Leon Levy Native Plant Preserve, Nassau, Bahamas. [Marcano-Vega, Humfredo] USDA Forest Serv, Southern Res Stn, Int Inst Trop Forestry, San Juan, PR 00926 USA. [Steadman, David W.] Univ Florida, Florida Museum Nat Hist, Gainesville, FL 32611 USA. RP Franklin, J (reprint author), Arizona State Univ, Sch Geog Sci & Urban Planning, Tempe, AZ 85287 USA. EM janet.franklin@asu.edu; julie.ripplinger@asu.edu; efreid@bnt.bs; hmarcano@fs.fed.us; dws@flmnh.ufl.edu RI Ripplinger, Julie/M-3213-2015 OI Ripplinger, Julie/0000-0002-2771-6637 FU U.S. National Science Foundation [BCS-1118340, BCS-1118369]; Arizona State University; USFS-Southern Research Station-FIA; USFS International Institute of Tropical Forestry FX This research was sponsored by the U.S. National Science Foundation (Grant BCS-1118340 to JF and BCS-1118369 to DWS) and Arizona State University (JF). For permissions and cooperation on Abaco and Eleuthera, we thank The Bahamas National Trust, The National Museum of The Bahamas (Antiquities, Monuments and Museums Corporation), Friends of the Environment, the Leon Levy Native Plant Preserve, C. Adair, M. and N. Albury, E. Carey, M. Daniels, A. and S. Knowles, D. Knowles, P. Maillis, O. Patterson, K. Tinker, and K. Williams. B. Trapido-Lurie produced Fig. 1. The USDA Forest Service (USFS) FIA program for Puerto Rico and the U.S. Virgin Islands is jointly funded by USFS-Southern Research Station-FIA and the USFS International Institute of Tropical Forestry. NR 62 TC 1 Z9 1 U1 7 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD JUN PY 2015 VL 216 IS 6 BP 873 EP 886 DI 10.1007/s11258-015-0474-8 PG 14 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CK4ED UT WOS:000356173000009 ER PT J AU Mitra, SK Chen, RQ Dhandaydham, M Wang, XF Blackburn, RK Kota, U Goshe, MB Schwartz, D Huber, SC Clouse, SD AF Mitra, Srijeet K. Chen, Ruiqiang Dhandaydham, Murali Wang, Xiaofeng Blackburn, Robert Kevin Kota, Uma Goshe, Michael B. Schwartz, Daniel Huber, Steven C. Clouse, Steven D. TI An autophosphorylation site database for leucine-rich repeat receptor-like kinases in Arabidopsis thaliana SO PLANT JOURNAL LA English DT Article DE receptor kinase; phosphorylation; Arabidopsis thaliana; mass spectrometry; motif analysis ID BRASSINOSTEROID SIGNAL-TRANSDUCTION; DEPENDENT PROTEIN-KINASE; PHOSPHORYLATION SITES; IN-VITRO; TYROSINE PHOSPHORYLATION; JUXTAMEMBRANE DOMAIN; FUNCTIONAL-ANALYSIS; THREONINE RESIDUES; CATALYTIC SUBUNIT; PLANT DEVELOPMENT AB Leucine-rich repeat receptor-like kinases (LRR RLKs) form a large family of plant signaling proteins consisting of an extracellular domain connected by a single-pass transmembrane sequence to a cytoplasmic kinase domain. Autophosphorylation on specific Ser and/or Thr residues in the cytoplasmic domain is often critical for the activation of several LRR RLK family members with proven functional roles in plant growth regulation, morphogenesis, disease resistance, and stress responses. While identification and functional characterization of invivo phosphorylation sites is ultimately required for a full understanding of LRR RLK biology and function, bacterial expression of recombinant LRR RLK cytoplasmic catalytic domains for identification of invitro autophosphorylation sites provides a useful resource for further targeted identification and functional analysis of invivo sites. In this study we employed high-throughput cloning and a variety of mass spectrometry approaches to generate an autophosphorylation site database representative of more than 30% of the approximately 223 LRR RLKs in Arabidopsis thaliana. We used His-tagged constructs of complete cytoplasmic domains to identify a total of 592 phosphorylation events across 73 LRR RLKs, with 497 sites uniquely assigned to specific Ser (268 sites) or Thr (229 sites) residues in 68 LRR RLKs. Multiple autophosphorylation sites per LRR RLK were the norm, with an average of seven sites per cytoplasmic domain, while some proteins showed more than 20 unique autophosphorylation sites. The database was used to analyze trends in the localization of phosphorylation sites across cytoplasmic kinase subdomains and to derive a statistically significant sequence motif for phospho-Ser autophosphorylation. Significance Statement High-throughput cloning and mass spectrometry were used to generate an extensive autophosphorylation site database of 496 unique phophosSer and phosphoThr sites for 68 Leucine-Rich Repeat Receptor-Like kinases in Arabidopsis thaliana. This database will be a useful resource for designing further experiments to characterize the biological function of multiple members of this large multi-gene family of plant signaling proteins. C1 [Mitra, Srijeet K.; Chen, Ruiqiang; Dhandaydham, Murali; Wang, Xiaofeng; Clouse, Steven D.] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Blackburn, Robert Kevin; Kota, Uma; Goshe, Michael B.] N Carolina State Univ, Dept Mol & Struct Biochem, Raleigh, NC 27695 USA. [Schwartz, Daniel] Univ Connecticut, Dept Physiol & Neurobiol, Storrs, CT 06269 USA. [Huber, Steven C.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. RP Clouse, SD (reprint author), N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. EM steve_clouse@ncsu.edu FU National Science Foundation [MCB-1021363, DBI-0619250, DBI-1126244]; North Carolina Agricultural Research Service FX This work was supported by grants from the National Science Foundation (MCB-1021363, DBI-0619250 and DBI-1126244) and the North Carolina Agricultural Research Service. Thanks to Gregory Scott for technical assistance. NR 61 TC 5 Z9 5 U1 5 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD JUN PY 2015 VL 82 IS 6 BP 1042 EP 1060 DI 10.1111/tpj.12863 PG 19 WC Plant Sciences SC Plant Sciences GA CK3BH UT WOS:000356089900012 PM 25912465 ER PT J AU Eckert, AJ Maloney, PE Vogler, DR Jensen, CE Mix, AD Neale, DB AF Eckert, Andrew J. Maloney, Patricia E. Vogler, Detlev R. Jensen, Camille E. Mix, Annette Delfino Neale, David B. TI Local adaptation at fine spatial scales: an example from sugar pine (Pinus lambertiana, Pinaceae) SO TREE GENETICS & GENOMES LA English DT Article DE Climate; Lake Tahoe Basin; Local adaptation; Pinus lambertiana; Soil; Sugar pine ID WATER-USE EFFICIENCY; HIERARCHICAL F-STATISTICS; SPRUCE PICEA-SITCHENSIS; GENE FLOW; ASSOCIATION GENETICS; QUANTITATIVE TRAITS; TREE POPULATIONS; DROUGHT STRESS; COMPLEX TRAITS; CLIMATE-CHANGE AB The forest environment can be heterogeneous over short physical distances, so that local adaptation by conifers, especially when selection is strong or gene flow restricted, may occur at fine spatial scales. Here, we examine patterns of genetic diversity across ten sugar pine (Pinus lambertiana Dougl.) populations located within the Lake Tahoe Basin of California and Nevada, USA. This region covers an area of only 1300 km(2) but is heterogeneous with respect to local environmental conditions. We associated 475 single nucleotide polymorphisms (SNPs) with five phenotypic traits, as well as with 11 environmental variables that describe the maternal tree environments. Phenotypes were measured in a common garden established from offspring of 108 maternal trees sampled from eight populations. Genetic, phenotypic, and environmental variation was pronounced among populations. Association genetic analysis identified six associations between SNPs and phenotypic traits, as well as 31 associations between SNPs and environmental principal components (PCs). Only two SNPs were shared among these sets of results. One of these two SNPs was associated with carbon isotope ratios that were correlated to an environmental PC representing soil and climate variables involved with water availability. Utilization of this three-pronged approach established conceptual links between genotypes and phenotypes in an ecological context. C1 [Eckert, Andrew J.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Maloney, Patricia E.; Jensen, Camille E.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Maloney, Patricia E.; Jensen, Camille E.] Univ Calif Davis, Tahoe Environm Res Ctr, Davis, CA 95616 USA. [Vogler, Detlev R.; Mix, Annette Delfino] US Forest Serv, USDA, Pacific Southwest Res Stn, Inst Forest Genet, Placerville, CA 95667 USA. [Neale, David B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Eckert, AJ (reprint author), Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. EM aeckert2@vcu.edu; pemaloney@ucdavis.edu FU Nevada Division of State Lands Lake Tahoe License Plate Program [LTLP 09-07] FX The Nevada Division of State Lands Lake Tahoe License Plate Program provided funding for this project (LTLP 09-07). NR 92 TC 2 Z9 2 U1 11 U2 53 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2015 VL 11 IS 3 AR 42 DI 10.1007/s11295-015-0863-0 PG 17 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CJ7UH UT WOS:000355704700010 ER PT J AU Friedline, CJ Lind, BM Hobson, EM Harwood, DE Mix, AD Maloney, PE Eckert, AJ AF Friedline, Christopher J. Lind, Brandon M. Hobson, Erin M. Harwood, Douglas E. Mix, Annette Delfino Maloney, Patricia E. Eckert, Andrew J. TI The genetic architecture of local adaptation I: the genomic landscape of foxtail pine (Pinus balfouriana Grev. & Balf.) as revealed from a high-density linkage map SO TREE GENETICS & GENOMES LA English DT Article DE Adaptation; Double digest restriction-site associated DNA sequencing; Foxtail pine; Linkage mapping; Pinus balfouriana ID QUANTITATIVE TRAIT LOCI; TAEDA L.; CRYPTOMERIA-JAPONICA; ASSOCIATION GENETICS; ARABIDOPSIS-THALIANA; GENOTYPING ERRORS; EVOLUTION; POPULATION; PINACEAE; CONIFER AB Explaining the origin and evolutionary dynamics of the genetic architecture of adaptation is a major research goal of evolutionary genetics. Despite controversy surrounding success of the attempts to accomplish this goal, a full understanding of adaptive genetic variation necessitates knowledge about the genomic location and patterns of dispersion for the genetic components affecting fitness-related phenotypic traits. Even with advances in next-generation sequencing technologies, the production of full genome sequences for non-model species is often costprohibitive, especially for tree species such as pines where genome size often exceeds 20 to 30 Gbp. We address this need by constructing a dense linkage map for foxtail pine (Pinus balfouriana Grev. & Balf.), with the ultimate goal of uncovering and explaining the origin and evolutionary dynamics of adaptive genetic variation in natural populations of this forest tree species. We utilized megagametophyte arrays (n = 76-95 megagametophytes/tree) from four maternal trees in combination with double digest restrictionsite associated DNA sequencing (ddRADseq) to produce a consensus linkage map covering 98.58 % of the foxtail pine genome, which was estimated to be 1276 cM in length (95 % CI, 1174 to 1378 cM). A novel bioinformatic approach using iterative rounds of marker ordering and imputation was employed to produce single-tree linkage maps (507-17,066 contigs/map; lengths, 1037.401572.80 cM). These linkage maps were collinear across maternal trees, with highly correlated marker orderings (Spearman's. > 0.95). A consensus linkage map derived from these single-tree linkage maps contained 12 linkage groups along which 20,655 contigs were non-randomly distributed across 901 unique positions (n = 23 contigs/ position), with an average spacing of 1.34 cM between adjacent positions. Of the 20,655 contigs positioned on the consensus linkage map, 5627 had enough sequence similarityto contigs contained within the most recent build of the loblolly pine (Pinus taeda L.) genome to identify them as putative homologues containing both genic and non-genic loci. Importantly, all 901 unique positions on the consensus linkage map had at least one contig with putative homology to loblolly pine. When combined with the other biological signals that predominate in our data (e. g., correlations of recombination fractions across single trees), we show that dense linkage maps for non-model forest tree species can be efficiently constructed using next-generation sequencing technologies. We subsequently discuss the usefulness of these maps as community-wide resources and as tools with which to test hypotheses about the genetic architecture of local adaptation.. C1 [Friedline, Christopher J.; Hobson, Erin M.; Harwood, Douglas E.; Eckert, Andrew J.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Lind, Brandon M.] Virginia Commonwealth Univ, Integrat Life Sci Program, Richmond, VA 23284 USA. [Mix, Annette Delfino] USDA, Inst Forest Genet, Pacific Southwest Res Stn, Placerville, CA USA. [Maloney, Patricia E.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Eckert, AJ (reprint author), Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. EM cfriedline@vcu.edu; lindb@vcu.edu; hobsonem@vcu.edu; harwoodde@vcu.edu; amix@fs.fed.us; pemaloney@ucdavis.edu; aeckert2@vcu.edu FU Virginia Commonwealth University; National Science Foundation (NSF) National Plant Genome Initiative (NPGI): Postdoctoral Research Fellowship in Biology (PRFB) [NSF-NPGI-PRFB-1306622] FX The authors would like to thank the staff at the USDA Institute of Forest Genetics, the VCU Nucleic Acids Research Facility, and the VCU Center for High Performance Computing. In addition, we would like to thank Tom Blush and Tom Burt for help in obtaining seeds. Funding for this project was made available to AJE via start-up funds from Virginia Commonwealth University. CJF was supported by the National Science Foundation (NSF) National Plant Genome Initiative (NPGI): Postdoctoral Research Fellowship in Biology (PRFB) FY 2013 Award no. NSF-NPGI-PRFB-1306622. NR 86 TC 2 Z9 2 U1 3 U2 18 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2015 VL 11 IS 3 DI 10.1007/s11295-015-0866-x PG 15 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CJ7UH UT WOS:000355704700017 ER PT J AU LaBonte, NR Ostry, ME Ross-Davis, A Woeste, KE AF LaBonte, Nicholas R. Ostry, Michael E. Ross-Davis, Amy Woeste, Keith E. TI Estimating heritability of disease resistance and factors that contribute to long-term survival in butternut (Juglans cinerea L.) SO TREE GENETICS & GENOMES LA English DT Article DE Ophiognomonia clavigignenti-juglandacearum; Butternut canker disease; Heritability; Forest genetics; Butternut ID PINE BLISTER RUST; SIROCOCCUS-CLAVIGIGNENTI-JUGLANDACEARUM; BEECH BARK DISEASE; NATURAL-POPULATIONS; MOLECULAR MARKERS; COMPUTER-PROGRAM; PAIRWISE RELATEDNESS; MICROSATELLITE LOCI; ARMILLARIA-MELLEA; GENETIC-STRUCTURE AB For most wild species affected by exotic pests or pathogens, the relative importance of heritable genetic differences in determining apparent variation in disease resistance is unknown. This is true in particular for butternut, a North American hardwood affected by butternut canker disease and undergoing demographic contraction. Little is known about site effects on butternut decline, in part because long-term monitoring data are lacking. We collected detailed disease phenotypes and multilocus microsatellite genotypes for all surviving individuals in a large natural population of butternut in 2003 (n=302) and 2012 (n=113). Two analytical methods, correlations between pairwise phenotypic similarity and pairwise relatedness, and estimation of among-family variance, both indicated weak heritability of disease-related traits and no heritability for overall tree health in the population. Additionally, an analysis of spatial data collected in 2001 (n=341) and 2012 (n=113) demonstrated that drier, upland sites contribute to increased likelihood of survival. We conclude that genetic differences among wild butternut individuals contributed little to observed variance in survival over 10 years but fine-scale site differences were useful predictors of butternut mortality. C1 [LaBonte, Nicholas R.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Ostry, Michael E.] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. [Ross-Davis, Amy] US Forest Serv, RMRS Forestry Sci Lab, USDA, Moscow, ID 83843 USA. [Woeste, Keith E.] Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, No Res Stn, USDA,Forest Serv, W Lafayette, IN 47907 USA. RP LaBonte, NR (reprint author), Purdue Univ, Dept Forestry & Nat Resources, Pfendler Hall,715 West State St, W Lafayette, IN 47907 USA. EM nlabonte@purdue.edu FU USDA NIFA [104355] FX The authors wish to thank Dwight Slocum for access to the butternuts at SLOW. Thanks also to Amy Miller and James Jacobs for the help in collecting leaf samples, and Megan Simmons for the help in extracting DNA and conducting PCR, and the staff of the Purdue Genomics Core for their advice and assistance. We would also like to thank J.S. Stanovick for the statistical support. The work presented in this paper was partially funded by the USDA NIFA Grant 104355 Partnership for Research and Education in Plant Breeding and Genetics at Purdue. We thank all the sponsors of the Partnership for their generosity. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 87 TC 0 Z9 0 U1 5 U2 21 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2015 VL 11 IS 3 AR 63 DI 10.1007/s11295-015-0884-8 PG 12 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CJ7UH UT WOS:000355704700031 ER PT J AU Padi, FK Ofori, A Takrama, J Djan, E Opoku, SY Dadzie, AM Bhattacharjee, R Motamayor, JC Zhang, DP AF Padi, Francis K. Ofori, Atta Takrama, Jemmy Djan, Esther Opoku, Stephen Y. Dadzie, Abu M. Bhattacharjee, Ranjana Motamayor, Juan Carlos Zhang, Dapeng TI The impact of SNP fingerprinting and parentage analysis on the effectiveness of variety recommendations in cacao SO TREE GENETICS & GENOMES LA English DT Article DE Diversity; Fingerprinting; Genetic structure; Mislabelling; Theobroma ID THEOBROMA-CACAO; NATURAL-POPULATIONS; GENETIC DIVERSITY; COCOA; L.; MICROSATELLITES; INCOMPATIBILITY; POLLINATION; ASSIGNMENT; INFERENCE AB Evidence for the impact of mislabeling and/or pollen contamination on consistency of field performance has been lacking to reinforce the need for strict adherence to quality control protocols in cacao seed garden and germplasm plot management. The present study used SNP fingerprinting at 64 loci to examine the diversity, labeling errors and parentage in 2551 trees obtained from six seed gardens, breeders clone collection and single-cross progenies and a sample of farmers' trees in Ghana. Clone mislabeling was pervasive, both within the seed garden clones and among clones of the breeders' active collection. Among the seed garden clones, mislabeled trees were assigned to other parental clones used in the seed garden, pointing to labeling errors prior to planting as the principal cause of mislabeling. Among the breeders' clone collection, both homonymous and synonymous mislabeling were identified in addition to trees with unique genotypes. This implicates pre-planting labeling errors and rootstocks overtaking budded scions. Parentage analysis supported the Amelonado ancestry of farmers' varieties but with significant contribution of Upper Amazon introductions. Parentage of recently developed clones and of progenies of controlled crosses showed evidence of both pollen contamination and effects of mislabeled parents. The observed patterns of unexpected parentage had direct effects on the consistency of the variety performance between trials and increased within-plot variability for families with mixed ancestry. The results provide a strong basis for mainstreaming SNP fingerprinting in cacao breeding programs to improve the efficiency of the variety development process. C1 [Padi, Francis K.; Ofori, Atta; Takrama, Jemmy; Djan, Esther; Opoku, Stephen Y.; Dadzie, Abu M.] Cocoa Res Inst, New Tafo Akim, Ghana. [Bhattacharjee, Ranjana] Int Inst Trop Agr IITA, Cent Biotechnol Lab, Ibadan, Nigeria. [Motamayor, Juan Carlos] Mars Inc, Hackettstown, NJ USA. [Zhang, Dapeng] USDA ARS PSI SPCL, Beltsville, MD 20705 USA. RP Padi, FK (reprint author), Cocoa Res Inst, Box 8, New Tafo Akim, Ghana. EM padifrancis@yahoo.co.uk FU Mars Incorporated through the African Cacao Initiative of the World Cacao Foundation (WCF/ACI); Ghana Cacao Board FX This research was financed in part by a grant from Mars Incorporated through the African Cacao Initiative of the World Cacao Foundation (WCF/ACI) and the Ghana Cacao Board. The paper is published with permission of the Executive Director of the Cacao Research Institute of Ghana as manuscript number CRIG/03/2015/028/001. NR 49 TC 1 Z9 1 U1 2 U2 17 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2015 VL 11 IS 3 AR 44 DI 10.1007/s11295-015-0875-9 PG 14 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CJ7UH UT WOS:000355704700012 ER PT J AU Potter, KM Hipkins, VD Mahalovich, MF Means, RE AF Potter, Kevin M. Hipkins, Valerie D. Mahalovich, Mary F. Means, Robert E. TI Nuclear genetic variation across the range of ponderosa pine (Pinus ponderosa): Phylogeographic, taxonomic and conservation implications SO TREE GENETICS & GENOMES LA English DT Article DE Biogeography; Gene conservation; Inbreeding; Isozymes; Microevolution; Microsatellites ID WESTERN UNITED-STATES; ALLELE FREQUENCY DATA; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; MITOCHONDRIAL-DNA; CLIMATE-CHANGE; ICE-AGES; POSTGLACIAL COLONIZATION; SPATIOTEMPORAL PATTERNS; HAPLOTYPE DISTRIBUTION AB Ponderosa pine (Pinus ponderosa) is among the most broadly distributed conifer species of western North America, where it possesses considerable ecological, esthetic, and commercial value. It exhibits complicated patterns of morphological and genetic variation, suggesting that it may be in the process of differentiating into distinct regional lineages. A robust analysis of genetic variation across the ponderosa pine complex is necessary to ensure the effectiveness of management and conservation efforts given the species' large distribution, the existence of many isolated disjunct populations, and the potential susceptibility of some populations to climate change and other threats. We used highly polymorphic nuclear microsatellite markers and isozyme markers from 3113 trees in 104 populations to assess genetic variation and structure across the geographic range of ponderosa pine. The results reveal pervasive inbreeding and patterns of genetic diversity consistent with the hypothesis that ponderosa existed in small, as-yet-undetected Pleistocene glacial refugia north of southern Arizona and New Mexico. The substructuring of genetic variation within the species complex was consistent with its division into two varieties, with genetic clusters within varieties generally associated with latitudinal zones. The analyses indicate widespread gene flow and/or recent common ancestry among genetic clusters within varieties, but not between varieties. Isolated disjunct populations had lower genetic variation by some measures and greater genetic differentiation than main-range populations. These results should be useful for decision-making and conservation planning related to this widespread and important species. C1 [Potter, Kevin M.] N Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC 27709 USA. [Hipkins, Valerie D.] US Forest Serv, Natl Forest Genet Lab, USDA, Placerville, CA 95667 USA. [Mahalovich, Mary F.] US Forest Serv, Genet Resource Program, Northern Rocky Mt Southwestern & Intermt Reg, USDA, Moscow, ID 83843 USA. [Means, Robert E.] Bur Land Management Wyoming, Cheyenne, WY 82009 USA. RP Potter, KM (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC 27709 USA. EM kpotter@ncsu.edu OI Potter, Kevin/0000-0002-7330-5345 FU Southern Research Station of the USDA Forest Service [10-JV-11330146-049]; North Carolina State University [10-JV-11330146-049] FX The authors thank the natural resource specialists from government agencies who assisted with the identification and collection of samples. The authors thank Doug Page, Connie Millar, and David Charlet for multiple collections; Jody Mello, Rosanna Hanson, Ricardo Hernandez, Pat Guge, Suellen Carroll, Kristin Motz, Courtney Owens, and Sara Trujillo for laboratory analyses; Mark Lesser for sharing the Pinus contorta primer sequences; and Julie Canavin for manuscript preparation assistance. This project was a cooperative effort between the Bureau of Land Management Wyoming and the National Forest Genetics Laboratory of the United States Department of Agriculture (USDA) Forest Service, Forest Management Staff. It was supported in part through Research Joint Venture Agreement 10-JV-11330146-049 between the Southern Research Station of the USDA Forest Service and North Carolina State University. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion or other products or vendors that also may be suitable. NR 134 TC 5 Z9 5 U1 3 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2015 VL 11 IS 3 AR 38 DI 10.1007/s11295-015-0865-y PG 23 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CJ7UH UT WOS:000355704700006 ER PT J AU Chen, J Nie, XA Liu, ZS Mi, Z Zhou, YH AF Chen, Jie Nie, Xiaoan Liu, Zengshe Mi, Zhen Zhou, Yonghong TI Synthesis and Application of Polyepoxide Cardanol Glycidyl Ether as Biobased Polyepoxide Reactive Diluent for Epoxy Resin SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Reactive diluents; Cardanol; Epoxy resin; Mechanical properties; Thermal properties ID RESPONSE-SURFACE METHODOLOGY; ACID METHYL-ESTERS; FATTY-ACID; OLEFIN EPOXIDATION; PROCESS VARIABLES; PHENOLIC RESIN; COATINGS; MECHANISMS; COMPOSITE; BLENDS AB Polyepoxide cardanol glycidyl ether (PECGE), a novel cardanol derivative, was synthesized and used as reactive diluent for petroleum-based epoxy resin in this work. The synthetic condition was first optimized, and the resultant PECGE diluent was characterized using Fourier transform infrared spectroscopy, H-1 NMR, and C-13 NMR. The influence of addition of this diluent on the viscosity of the diluent epoxy resin was also studied. Mechanical and heat-resistant properties of the cured epoxy resin containing PECGE were especially evaluated. By the addition of PECGE into the petroleum-based epoxy resin, the viscosity of the obtained epoxy resin was reduced. The tensile strength, elongation at break, and heat-resistant property of the obtained resin were all improved, while the flexural and compressive strengths of the resin only slightly decreased, implying the potential of PECGE as a very promising biobased reactive diluent for epoxy resin. C1 [Chen, Jie; Nie, Xiaoan; Mi, Zhen; Zhou, Yonghong] CAF, Inst Chem Ind Forestry Prod, Nanjing 210042, Jiangsu, Peoples R China. [Chen, Jie; Nie, Xiaoan; Mi, Zhen; Zhou, Yonghong] Natl Engn Lab Biomass Chem Utilizat, Nanjing 210042, Jiangsu, Peoples R China. [Chen, Jie; Nie, Xiaoan; Mi, Zhen; Zhou, Yonghong] Key Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China. [Nie, Xiaoan; Zhou, Yonghong] CAF, Inst New Technol Forestry, Beijing 100091, Peoples R China. [Liu, Zengshe] USDA ARS, Natl Ctr Agr Utilizat Res, Bio Oils Res Unit, Peoria, IL 61604 USA. RP Liu, ZS (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Bio Oils Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. EM kevin.liu@ars.usda.gov FU National "Twelth Five-Year" Plan for Science & Technology Support [2014BAD02B02]; Basic research funding earmarked for the national commonweal research institutes, CAF [CAFINT2014C12] FX The authors are grateful to the financial support from National "Twelth Five-Year" Plan for Science & Technology Support (grant number: 2014BAD02B02) and Basic research funding earmarked for the national commonweal research institutes, CAF (grant number: CAFINT2014C12). NR 44 TC 9 Z9 10 U1 5 U2 41 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD JUN PY 2015 VL 3 IS 6 BP 1164 EP 1171 DI 10.1021/acssuschemeng.5b00095 PG 8 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA CJ6ZZ UT WOS:000355645000016 ER PT J AU Baranowski, T AF Baranowski, Tom TI Are active video games useful to combat obesity? SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Editorial Material ID PHYSICAL-ACTIVITY; TRIAL; YOUTH; TIME; CHILDREN; IMPACT C1 Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU NCI NIH HHS [CA158917, CA 172864]; NICHD NIH HHS [HD075521, HD073608]; NIDDK NIH HHS [(DK091254-01] NR 13 TC 2 Z9 2 U1 0 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1107 EP 1108 DI 10.3945/ajcn.115.110221 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900001 PM 25809852 ER PT J AU Balentine, DA Dwyer, JT Erdman, JW Ferruzzi, MG Gaine, PC Hamly, JM Kwik-Uribe, CL AF Balentine, Douglas A. Dwyer, Johanna T. Erdman, John W., Jr. Ferruzzi, Mario G. Gaine, P. Courtney Hamly, James M. Kwik-Uribe, Catherine L. TI Recommendations on reporting requirements for flavonoids in research SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE bioactives; guidance; intervention; polyphenols; supplements ID CONCORD GRAPE JUICE; PERFORMANCE LIQUID-CHROMATOGRAPHY; CARDIOVASCULAR-DISEASE MORTALITY; HYDROXYCINNAMIC ACID-DERIVATIVES; FOOD FREQUENCY QUESTIONNAIRE; CORONARY-ARTERY-DISEASE; PARA-AMINOBENZOIC ACID; DAD-ESI-MSN; PHENOLIC-COMPOUNDS; HEART-DISEASE AB Numerous observational and intervention-based human studies support the notion of a beneficial role for dietary flavonoids in human health. Despite these studies, it is not yet possible to make dietary recommendations with regard to the types and amounts of flavonoids to be consumed. The inherent diversity of flavonoid structure, chemistry, and natural distribution in foods lends itself to errors in reporting the types and/or amounts of flavonoids consumed, as well as incomplete recognition of requirements for intervention studies that aim to assess their benefits in a clinical setting. A need exists for guidelines that facilitate the design and reporting of flavonoid research. With a focus on clinical studies, this article I) outlines limitations commonly encountered in the field of flavonoid research, including the inconsistent use of nomenclature, inappropriate analytic methods, inconsistent use of existing flavonoid databases, and the lack of full consideration in the design of test materials for intervention trials, and 2) provides guidance for future studies with a focus on clinical intervention trials. Adoption of this guidance will facilitate more accurate and interpretable research that will support the development of dietary recommendations regarding the intake of flavonoids. C1 [Balentine, Douglas A.] Unilever, Englewood Cliffs, NJ USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Erdman, John W., Jr.] Univ Illinois, Urbana, IL 61801 USA. [Ferruzzi, Mario G.] Purdue Univ, W Lafayette, IN 47907 USA. [Gaine, P. Courtney] Sugar Assoc, Washington, DC USA. [Hamly, James M.] USDA ARS, Beltsville, MD USA. [Kwik-Uribe, Catherine L.] Mars Inc, Mclean, VA USA. RP Erdman, JW (reprint author), Univ Illinois, 449 Bevier Hall,905 S Goodwin Ave, Urbana, IL 61801 USA. EM jwerdman@illinois.edu OI Dwyer, Johanna/0000-0002-0783-1769 FU North American Branch of the International Life Sciences Institute, Technical Committee on Flavonoids FX Supported by the North American Branch of the International Life Sciences Institute, Technical Committee on Flavonoids, for the coordination of the writing and open access fees for publication of this article. This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelines-and-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. NR 97 TC 9 Z9 9 U1 1 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1113 EP 1125 DI 10.3945/ajcn.113.071274 PG 13 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900004 PM 25854881 ER PT J AU Stanhope, KL Medici, V Bremer, AA Lee, V Lam, HD Nunez, MV Chen, GX Keim, NL Havel, PJ AF Stanhope, Kimber L. Medici, Valentina Bremer, Andrew A. Lee, Vivien Lam, Hazel D. Nunez, Marinelle V. Chen, Guoxia X. Keim, Nancy L. Havel, Peter J. TI A dose-response study of consuming high-fructose corn syrup-sweetened beverages on lipid/lipoprotein risk factors for cardiovascular disease in young adults SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE apolipoprotein CIII; fructose; risk factors; sugar; uric acid ID SERUM URIC-ACID; RANDOMIZED CONTROLLED-TRIAL; INSULIN SENSITIVITY; METABOLIC SYNDROME; BLOOD-LIPIDS; LIVER FAT; POSTPRANDIAL TRIGLYCERIDES; ENERGY-EXPENDITURE; VASCULAR-DISEASE; ORAL FRUCTOSE AB Background: National Health and Nutrition Examination Survey data show an increased risk of cardiovascular disease (CVD) mortality with an increased intake of added sugar. Objective: We determined the dose-response effects of consuming beverages sweetened with high-fructose corn syrup (HFCS) at zero, low, medium, and high proportions of energy requirements (Ereq) on circulating lipid/lipoprotein risk factors for CVD and uric acid in adults [age: 18-40 y; body mass index (in kg/m(2)): 18-35]. Design: We conducted a parallel-arm, nonrandomized, double-blinded intervention study in which adults participated in 3.5 inpatient days of baseline testing at the University of California Davis Clinical and Translational Science Center's Clinical Research Center. Participants then consumed beverages sweetened with HFCS at 0% (aspartame sweetened, n = 23), 10% (n = 18), 17.5% (n = 16), or 25% (n = 28) of Ereq during 13 outpatient days and during 3.5 inpatient days of intervention testing at the research center. We conducted 24-h serial blood collections during the baseline and intervention testing periods. Results: Consuming beverages containing 10%, 17.5%, or 25% Ereq from HFCS produced significant linear dose-response increases of lipid/lipoprotein risk factors for CVD and uric acid: postprandial triglyceride (0%: 0 4; 10%: 22 8; 17.5%: 25 5: 25%: 37 +/- 5 mg/dL, mean of Delta +/- SE, P < 0.0001 effect of HFCS-dose), fasting LDL cholesterol (0%: -1.0 +/- 3.1; 10%: 7.4 +/- 3.2; 17.5%: 8.2 +/- 3.1; 25%: 15.9 +/- 3.1 mg/dL, P < 0.0001), and 24-h mean uric acid concentrations (0%: -0.13 +/- 0.07; 10%: 0.15 +/- 0.06; 17.5%: 0.30 +/- 0.07; 25%: 0.59 +/- 0.09 mg/dL, P < 0.0001). Compared with beverages containing 0% HFCS, all 3 doses of HFCS-containing beverages increased concentrations of postprandial triglyceride, and the 2 higher doses increased fasting and/or postprandial concentrations of non-HDL cholesterol, LDL cholesterol, apolipoprotein B, apolipoprotein CIII, and uric acid. Conclusions: Consuming beverages containing 10%, 17.5%, or 25% Ereq from HFCS produced dose-dependent increases in circulating lipid/lipoprotein risk factors for CVD and uric acid within 2 wk. These results provide mechanistic support for the epidemiologic evidence that the risk of cardiovascular mortality is positively associated with consumption of increasing amounts of added sugars. This trial was registered at clinicaltrials.gov as NCT01103921. C1 [Stanhope, Kimber L.; Lee, Vivien; Lam, Hazel D.; Havel, Peter J.] Univ Calif Davis, Dept Mol Sci, Sch Vet Med, Davis, CA 95616 USA. [Stanhope, Kimber L.; Nunez, Marinelle V.; Keim, Nancy L.; Havel, Peter J.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Medici, Valentina] Univ Calif Davis, Div Gastroenterol & Hepatol, Sch Med, Davis, CA 95616 USA. [Bremer, Andrew A.] Univ Calif Davis, Dept Pediat, Sch Med, Davis, CA 95616 USA. [Keim, Nancy L.] Western Human Nutr Res Ctr, USDA, Davis, CA USA. RP Stanhope, KL (reprint author), Univ Calif Davis, Dept Mol Sci, Sch Vet Med, One Shields Ave, Davis, CA 95616 USA. EM klstanhope@ucdavis.edu FU NIH/National Heart, Lung and Blood Institute [1R01 HL09133, 1R01 HL107256]; National Center for Research Resources, a component of the NIH; NIH Roadmap for Medical Research [UL1 RR024146]; Building Interdisciplinary Research Careers in Women's Health award - National Institute of Child Health and Human Development, Office of Research on Women's Health, Office of Dietary Supplements [K12 HD051958]; National Institute of Aging; intramural USDA-ARS CRIS [5306-51530-022-00D] FX Supported by the NIH/National Heart, Lung and Blood Institute 1R01 HL09133 and 1R01 HL107256; National Center for Research Resources, a component of the NIH, and NIH Roadmap for Medical Research UL1 RR024146; a Building Interdisciplinary Research Careers in Women's Health award (K12 HD051958), funded by the National Institute of Child Health and Human Development, Office of Research on Women's Health, Office of Dietary Supplements, and the National Institute of Aging (to KLS); and intramural USDA-ARS CRIS 5306-51530-022-00D (to NLK). NR 51 TC 28 Z9 28 U1 6 U2 52 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1144 EP 1154 DI 10.3945/ajcn.114.100461 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900007 PM 25904601 ER PT J AU Layman, DK Anthony, TG Rasmussen, BB Adams, SH Lynch, CJ Brinkworth, GD Davis, TA AF Layman, Donald K. Anthony, Tracy G. Rasmussen, Blake B. Adams, Sean H. Lynch, Christopher J. Brinkworth, Grant D. Davis, Teresa A. TI Defining meal requirements for protein to optimize metabolic roles of amino acids SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT Foundation for Protein Summit 2.0 CY OCT 02-03, 2013 CL Purdue Univ, Washington, DC HO Purdue Univ DE leucine; mTOR; muscle protein synthesis; nitrogen balance; satiety ID HUMAN SKELETAL-MUSCLE; RANDOMIZED CONTROLLED-TRIAL; TYPE-2 DIABETES-MELLITUS; REGULATES FOOD-INTAKE; WEIGHT-LOSS DIETS; RESISTANCE EXERCISE; OLDER-ADULTS; LEAN MASS; BED REST; ANABOLIC RESPONSE AB Dietary protein provides essential amino acids (EAAs) for the synthesis of new proteins plus an array of other metabolic functions; many of these functions are sensitive to postprandial plasma and intracellular amino acid concentrations. Recent research has focused on amino acids as metabolic signals that influence the rate of protein synthesis, inflammation responses, mitochondrial activity, and satiety, exerting their influence through signaling systems including mammalian/mechanistic target of rapamycin complex 1 (mTORC1), general control nonrepressed 2 (GCN2), glucagon-like peptide 1 (GLP-1), peptide YY (PYY), serotonin, and insulin. These signals represent meal-based responses to dietary protein. The best characterized of these signals is the leucine-induced activation of mTORC1, which leads to the stimulation of skeletal muscle protein synthesis after ingestion of a meal that contains protein. The response of this metabolic pathway to dietary protein (i.e., meal threshold) declines with advancing age or reduced physical activity. Current dietary recommendations for protein are focused on total daily intake of 0.8 g/kg body weight, but new research suggests daily needs for older adults of >= 1.0 g/kg and identifies anabolic and metabolic benefits to consuming at least 20-30 g protein at a given meal. Resistance exercise appears to increase the efficiency of EAA use for muscle anabolism and to lower the meal threshold for stimulation of protein synthesis. Applying this information to a typical 3-meal-a-day dietary plan results in protein intakes that are well within the guidelines of the Dietary Reference Intakes for acceptable macronutrient intakes. The meal threshold concept for dietary protein emphasizes a need for redistribution of dietary protein for optimum metabolic health. C1 [Layman, Donald K.] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Anthony, Tracy G.] Rutgers State Univ, Dept Nutrit Sci, New Brunswick, NJ 08903 USA. [Rasmussen, Blake B.] Univ Texas Med Branch, Dept Nutr & Metab, Div Rehabil Sci, Galveston, TX 77555 USA. [Rasmussen, Blake B.] Univ Texas Med Branch, Sealy Ctr Aging, Galveston, TX 77555 USA. [Adams, Sean H.] Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA. [Adams, Sean H.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. [Lynch, Christopher J.] Penn State Univ, Coll Med, Dept Cellular & Mol Physiol, Hershey, PA USA. [Brinkworth, Grant D.] Commonwealth Sci & Ind Res Org Food & Nutrit Sci, Adelaide, SA, Australia. [Davis, Teresa A.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. RP Layman, DK (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, 260 Bevier Hall,905 South Goodwin, Urbana, IL 61801 USA. EM dlayman@illinois.edu OI Rasmussen, Blake/0000-0001-7854-5375 FU Beef Checkoff; Dairy Research Institute; Egg Nutrition Center; Global Dairy Platform; Hillshire Brands; National Pork Board; USDA-Agricultural Research Service [5306-51530-019-00]; National Dairy Council [58-5306-2-197]; NIH-National Institute of Diabetes and Digestive and Kidney Diseases [R01DK078328] FX Protein Summit 2.0 and this supplement were supported by funding from The Beef Checkoff, Dairy Research Institute, Egg Nutrition Center, Global Dairy Platform, Hillshire Brands, and the National Pork Board. Support for research relevant to this review was provided by the USDA-Agricultural Research Service intramural project 5306-51530-019-00, a grant (58-5306-2-197) from the National Dairy Council administered by Dairy Research Inc., and NIH-National Institute of Diabetes and Digestive and Kidney Diseases R01DK078328 (SHA). This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelines-and-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. NR 119 TC 18 Z9 19 U1 6 U2 34 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1330S EP 1338S DI 10.3945/ajcn.114.084053 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900032 ER PT J AU Paddon-Jones, D Campbell, WW Jacques, PF Kritchevsky, SB Moore, LL Rodriguez, NR van Loon, LJC AF Paddon-Jones, Douglas Campbell, Wayne W. Jacques, Paul F. Kritchevsky, Stephen B. Moore, Lynn L. Rodriguez, Nancy R. van Loon, Luc J. C. TI Protein and healthy aging SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT Foundation for Protein Summit 2.0 CY OCT 02-03, 2013 CL Purdue Univ, Washington, DC HO Purdue Univ DE dietary requirements; muscle; nutrition; protein; sarcopenia ID RECOMMENDED DIETARY ALLOWANCE; PLACEBO-CONTROLLED TRIAL; TRAINING-INDUCED CHANGES; ESSENTIAL AMINO-ACIDS; FRAIL ELDERLY-PEOPLE; OLDER-ADULTS; SKELETAL-MUSCLE; RESISTANCE EXERCISE; LEUCINE SUPPLEMENTATION; ANABOLIC RESPONSE AB Our understanding of the potential benefits and challenges of optimizing dietary protein intake in older adults continues to evolve. An overarching hypothesis generated during Protein Summit 2.0 was that consuming an adequate amount of high-quality protein at each meal, in combination with physical activity, may, delay the onset of sarcopenia, slow its progression, reduce the magnitude of its functional consequences, or all of these. The potential benefits of young and middle-aged adults adopting a diet pattern whereby adequate protein is consumed at each meal as a countermeasure to sarcopenia are presented and discussed. For example, meeting a protein threshold (similar to 25-30 g/meal) represents a promising, yet still largely unexplored dietary strategy to help maintain muscle mass and function. For many older adults, breakfast is a carbohydrate-dominated lower-protein meal and represents an opportunity to improve and more evenly distribute daily protein intake. Although both animal and plant-based proteins can provide the required essential amino acids for health, animal proteins generally have a higher proportion of the amino acid leucine. Leucine plays a key role in stimulating translation initiation and muscle protein anabolism and is the focus of ongoing research. Protein requirements should be assessed in the light of habitual physical activity. An evenly distributed protein diet provides a framework that allows older adults to benefit from the synergistic anabolic effect of protein and physical activity. To fully understand the role of dietary protein intake in healthy aging, greater efforts are needed to coordinate and integrate research design and data acquisition and interpretation from a variety of disciplines. C1 [Paddon-Jones, Douglas] Univ Texas Med Branch, Dept Nutr & Metab, Galveston, TX 77555 USA. [Campbell, Wayne W.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. [Jacques, Paul F.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. [Kritchevsky, Stephen B.] Wake Forest Sch Med, Gerontol & Geriatr Med, Winston Salem, NC USA. [Moore, Lynn L.] Boston Univ, Dept Med, Boston, MA 02215 USA. [Rodriguez, Nancy R.] Univ Connecticut, Dept Nutrit Sci, Storrs, CT USA. [van Loon, Luc J. C.] Maastricht Univ, NUTRIM, Sch Nutr Toxicol & Metab, NL-6200 MD Maastricht, Netherlands. RP Paddon-Jones, D (reprint author), Univ Texas Med Branch, Dept Nutr & Metab, 301 Univ Blvd, Galveston, TX 77555 USA. EM djpaddon@utmb.edu FU Beef Checkoff; Dairy Research Institute; Egg Nutrition Center; Global Dairy Platform; Hillshire Brands; National Pork Board FX Protein Summit 2.0 and this supplement were supported by funding from The Beef Checkoff, Dairy Research Institute, Egg Nutrition Center, Global Dairy Platform, Hillshire Brands, and the National Pork Board. Responsibility for the design, implementation, analysis, and interpretation of the information presented in this review was that of the authors. This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelines-and-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. NR 78 TC 20 Z9 20 U1 11 U2 48 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1339S EP 1345S DI 10.3945/ajcn.114.084061 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900033 ER PT J AU Phillips, SM Fulgoni, VL Heaney, RP Nicklas, TA Slavin, JL Weaver, CM AF Phillips, Stuart M. Fulgoni, Victor L., III Heaney, Robert P. Nicklas, Theresa A. Slavin, Joanne L. Weaver, Connie M. TI Commonly consumed protein foods contribute to nutrient intake, diet quality, and nutrient adequacy SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT Foundation for Protein Summit 2.0 CY OCT 02-03, 2013 CL Purdue Univ, Washington, DC HO Purdue Univ DE diet quality; mineral; nutrient density; vitamin; nutrient rich; animal protein ID NUTRITION EXAMINATION SURVEY; ADULTS NATIONAL-HEALTH; NUT CONSUMPTION; KEY NUTRIENTS; UNITED-STATES; RICH FOODS; US ADULTS; INDEX; PATTERNS; POSITION AB The amount of dietary protein needed to prevent deficiency in most individuals is defined in the United States and Canada by the Recommended Dietary Allowance and is currently set at 0.8 g protein . kg(-1) . d(-1) for adults. To meet this protein recommendation, the intake of a variety of protein food sources is advised. The goal of this article is to show that commonly consumed food sources of protein are more than just protein but also significant sources of essential nutrients. Commonly consumed sources of dietary protein frequently contribute substantially to intakes of nutrients such as calcium, vitamin D, potassium, dietary fiber, iron, and folate, which have been identified as nutrients of "concern" (i.e., intakes are often lower than recommended). Despite this, dietary recommendations to reduce intakes of saturated fat and solid fats may result in dietary guidance to reduce intakes of commonly consumed food sources of protein, in particular animal-based protein. We propose that following such dietary guidance would make it difficult to meet recommended intakes for a number of nutrients, at least without marked changes in dietary consumption patterns. These apparently conflicting pieces of dietary guidance are hard to reconcile; however, we view it as prudent to advise the intake of high-quality dietary protein to ensure adequate intakes of a number of nutrients, particularly nutrients of concern. C1 [Phillips, Stuart M.] McMaster Univ, Hamilton, ON, Canada. [Fulgoni, Victor L., III] Nutr Impact LLC, Battle Creek, MI USA. [Heaney, Robert P.] Creighton Univ, Omaha, NE 68178 USA. [Nicklas, Theresa A.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Slavin, Joanne L.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN USA. [Weaver, Connie M.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. RP Phillips, SM (reprint author), McMaster Univ, Dept Kinesiol, Exercise Metab Res Grp, Hamilton, ON, Canada. EM phillis@mcmaster.ca FU Beef Checkoff; Dairy Research Institute; Egg Nutrition Center; Global Dairy Platform; Hillshire Brands; National Pork Board; USDA/Agricultural Research Service [58-6250-0-008] FX Protein Summit 2.0 and this supplement were supported by funding from The Beef Checkoff, Dairy Research Institute, Egg Nutrition Center, Global Dairy Platform, Hillshire Brands, and the National Pork Board. Responsibility for the design, implementation, analysis, and interpretation of the information presented in this review was that of the authors.; Supported in part by the USDA/Agricultural Research Service through specific cooperative agreement 58-6250-0-008 (to TAN). No other specific sources of funding supported this work. This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelines-and-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. NR 48 TC 9 Z9 9 U1 3 U2 21 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1346S EP 1352S DI 10.3945/ajcn.114.084079 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900034 ER PT J AU Alexanden, N Rowe, S Brackett, RE Burton-Freeman, B Hentges, EJ Kretser, A Kluffeld, DM Meyers, LD Mukherjea, R Ohlhorst, S AF Alexanden, Nick Rowe, Sylvia Brackett, Robert E. Burton-Freeman, Britt Hentges, Eric J. Kretser, Alison Kluffeld, David M. Meyers, Linda D. Mukherjea, Ratna Ohlhorst, Sarah TI Achieving a transparent, actionable framework for public-private partnerships for food and nutrition research SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article; Proceedings Paper CT Foundation for Protein Summit 2.0 CY OCT 02-03, 2013 CL Purdue Univ, Washington, DC HO Purdue Univ DE public-private partnerships; research; guiding principles; nutrition; food safety; public health; conflict of interest AB Officers and other representatives of more than a dozen food-, nutrition-, and health-related scientific societies and organizations, food industry scientists, and staff of the USDA, the CDC, the Food and Drug Administration, and the NIH convened on 8 December 2014 in Washington, DC, to reach a consensus among individuals participating on guiding principles for the development of research-oriented, food-and nutrition-related public-private partnerships. During the daylong working meeting, participants discussed and revised 12 previously published guidelines to ensure integrity in the conduct of food and nutrition research collaborations among public, nonprofit, and private sectors. They agreed to reconvene periodically to reassess the public-private partnership principles. This article presents the guiding principles and potential benefits, outlines key discussion points, and articulates points of agreement and reservation. C1 [Alexanden, Nick] Media Concerns, Lewes, DE USA. [Rowe, Sylvia] SR Strategy LLC, Washington, DC USA. [Brackett, Robert E.; Burton-Freeman, Britt] IIT, Inst Food Safety & Hlth, Chicago, IL 60616 USA. [Hentges, Eric J.; Kretser, Alison] Int Life Sci Inst North Amer, Washington, DC USA. [Kluffeld, David M.] USDA ARS, Beltsville, MD USA. [Meyers, Linda D.; Ohlhorst, Sarah] Amer Soc Nutr, Bethesda, MD USA. [Mukherjea, Ratna] DuPont Nutr & Hlth, St Louis, MO USA. RP Ohlhorst, S (reprint author), Amer Soc Nutr, Bethesda, MD USA. EM sohlhorst@nutrition.org FU USDA Agricultural Research Service [58-1235-3-124] FX Supported in part by USDA Agricultural Research Service Cooperative Agreement 58-1235-3-124 to the ASN. This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelines-and-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. NR 1 TC 8 Z9 8 U1 2 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2015 VL 101 IS 6 BP 1359 EP 1363 DI 10.3945/ajcn.115.112805 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6YU UT WOS:000355641900036 PM 26034107 ER PT J AU Bosco-Lauth, AM Panella, NA Root, JJ Gidlewski, T Lash, RR Harmon, JR Burkhalter, KL Godsey, MS Savage, HM Nicholson, WL Komar, N Brault, AC AF Bosco-Lauth, Angela M. Panella, Nicholas A. Root, J. Jeffrey Gidlewski, Tom Lash, R. Ryan Harmon, Jessica R. Burkhalter, Kristen L. Godsey, Marvin S. Savage, Harry M. Nicholson, William L. Komar, Nicholas Brault, Aaron C. TI Serological Investigation of Heartland Virus (Bunyaviridae: Phlebovirus) Exposure in Wild and Domestic Animals Adjacent to Human Case Sites in Missouri 2012-2013 SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID THROMBOCYTOPENIA SYNDROME VIRUS; SEVERE FEVER; AMBLYOMMA-AMERICANUM; SHANDONG PROVINCE; UNITED-STATES; RIO-GRANDE; CHINA; TICK; IDENTIFICATION; TRANSMISSION AB Heartland virus (HRTV; Bunyaviridae: Phlebovirus) has recently emerged as a causative agent of human disease characterized by thrombocytopenia and leukopenia in the United States. The lone star tick (Amblyomma americanum L.) has been implicated as a vector. To identify candidate vertebrate amplification hosts associated with enzootic maintenance of the virus, sera and ticks were sampled from 160 mammals (8 species) and 139 birds (26 species) captured near 2 human case residences in Andrew and Nodaway Counties in northwest Missouri. HRTV-specific neutralizing antibodies were identified in northern raccoons (42.6%), horses (17.4%), white-tailed deer (14.3%), dogs (7.7%), and Virginia opossums (3.8%), but not in birds. Virus isolation attempts from sera and ticks failed to detect HRTV. The high antibody prevalence coupled with local abundance of white-tailed deer and raccoons identifies these species as candidate amplification hosts. C1 [Bosco-Lauth, Angela M.; Panella, Nicholas A.; Burkhalter, Kristen L.; Godsey, Marvin S.; Savage, Harry M.; Komar, Nicholas; Brault, Aaron C.] Ctr Dis Control & Prevent, Div Vector Borne Dis, Arboviral Dis Branch, Ft Collins, CO 80521 USA. [Root, J. Jeffrey; Gidlewski, Tom] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Lash, R. Ryan; Harmon, Jessica R.; Nicholson, William L.] Ctr Dis Control & Prevent, Div Vector Borne Dis, Rickettsial Dis Branch, Ft Collins, CO 80521 USA. RP Brault, AC (reprint author), Ctr Dis Control & Prevent, Div Vector Borne Dis, 3156 Rampart Rd, Ft Collins, CO 80521 USA. EM mopargal@rams.colostate.edu; nap4@cdc.gov; Jeff.Root@aphis.usda.gov; Thomas.Gidlewski@aphis.usda.gov; rlash@cdc.gov; jharmon@cdc.gov; ktb3@cdc.gov; mjg9@cdc.gov; hms1@cdc.gov; wnicholson@cdc.gov; nck6@cdc.gov; abrault@cdc.gov FU American Society of Microbiology (ASM) FX Funding for Angela Bosco-Lauth was provided by a fellowship from the American Society of Microbiology (ASM). NR 27 TC 7 Z9 7 U1 2 U2 9 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA SN 0002-9637 EI 1476-1645 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD JUN PY 2015 VL 92 IS 6 BP 1163 EP 1167 DI 10.4269/mtmh.14-0702 PG 5 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA CJ8WY UT WOS:000355785400014 PM 25870419 ER PT J AU Ridpath, JF Neill, JD AF Ridpath, Julia F. Neill, John D. TI Pestiviruses: old enemies and new challenges SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Editorial Material DE pestiviruses; eradication; emerging; impact; US BVDV Symposium; ESVV Pestivirus Meeting AB The genesis for this special issue on pestiviruses was a joint meeting on pestiviruses organized by the US BVDV Symposia Committee and the European Society for Veterinary Virology that was held October 14 and 15, 2014 in Kansas City, MO. The theme of the meeting was Pestiviruses: Old enemies and new challenges. The impetus for this joint effort was the recognition that regional approaches to disease control are at odds with the worldwide traffic in animal products and biologics. Further, the control of newly recognized pestiviruses, such as HoBi-like viruses, is more effective when approached as a global challenge rather than any one nation's problem. The joint meeting featured talks by researchers from North America, South America, Australia and Europe. The papers in this issue arose from keynote talks presented at the joint meeting and are organized around the following themes; pestiviruses and the immune system, genetic variability, the emergence of new pestiviruses and pestivirus control programs. C1 [Ridpath, Julia F.; Neill, John D.] USDA ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. RP Ridpath, JF (reprint author), USDA ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. EM julia.ridpath@ars.usda.gov NR 1 TC 1 Z9 1 U1 1 U2 6 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 EI 1475-2654 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD JUN PY 2015 VL 16 IS 1 BP 1 EP 3 DI 10.1017/S1466252315000134 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA CK1CV UT WOS:000355943900001 PM 26050566 ER PT J AU Hansen, TR Smirnova, NP Webb, BT Bielefeldt-Ohmann, H Sacco, RE Van Campen, H AF Hansen, Thomas R. Smirnova, Natalia P. Webb, Brett T. Bielefeldt-Ohmann, Helle Sacco, Randy E. Van Campen, Hana TI Innate and adaptive immune responses to in utero infection with bovine viral diarrhea virus SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Review DE innate; adaptive; immune response; bovine viral diarrhea virus; persistent infection; interferon; IFN; fetus ID DOUBLE-STRANDED-RNA; INTERFERON REGULATORY FACTOR-3; FETAL PERSISTENT INFECTION; IFN-GAMMA; PROTEASOMAL DEGRADATION; ALPHA/BETA-INTERFERON; TRANSCRIPTION FACTORS; RESPIRATORY-DISEASE; BETA-INTERFERON; HOST-DEFENSE AB Infection of pregnant cows with noncytopathic (ncp) bovine viral diarrhea virus (BVDV) induces rapid innate and adaptive immune responses, resulting in clearance of the virus in less than 3 weeks. Seven to 14 days after inoculation of the cow, ncpBVDV crosses the placenta and induces a fetal viremia. Establishment of persistent infection with ncpBVDV in the fetus has been attributed to the inability to mount an immune response before 90-150 days of gestational age. The result is immune tolerance', persistent viral replication and shedding of ncpBVDV. In contrast, we describe the chronic upregulation of fetal Type I interferon (IFN) pathway genes and the induction of IFN- pathways in fetuses of cows infected on day 75 of gestation. Persistently infected (PI) fetal IFN- concentrations also increased at day 97 at the peak of fetal viremia and IFN- mRNA was significantly elevated in fetal thymus, liver and spleen 14-22 days post maternal inoculation. PI fetuses respond to ncpBVDV infection through induction of Type I IFN and IFN- activated genes leading to a reduction in ncpBVDV titer. We hypothesize that fetal infection with BVDV persists because of impaired induction of IFN- in the face of activated Type I IFN responses. Clarification of the mechanisms involved in the IFN-associated pathways during BVDV fetal infection may lead to better detection methods, antiviral compounds and selection of genetically resistant breeding animals. C1 [Hansen, Thomas R.; Smirnova, Natalia P.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Biomed Sci, Anim Reprod & Biotechnol Lab, Ft Collins, CO 80523 USA. [Webb, Brett T.] N Dakota State Univ, Vet Diagnost Lab, Fargo, ND 58108 USA. [Bielefeldt-Ohmann, Helle] Univ Queensland, Australian Infect Dis Res Ctr, Brisbane, Qld 4072, Australia. [Bielefeldt-Ohmann, Helle] Univ Queensland, Sch Vet Sci, Brisbane, Qld 4072, Australia. [Sacco, Randy E.] USDA ARS, Ruminant Dis & Immunol Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Van Campen, Hana] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. RP Hansen, TR (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Biomed Sci, Anim Reprod & Biotechnol Lab, Ft Collins, CO 80523 USA. EM thomas.hansen@colostate.edu FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2008-35204-04652] FX This project was supported by Agriculture and Food Research Initiative Competitive (Grant no. 2008-35204-04652) from the USDA National Institute of Food and Agriculture. NR 75 TC 3 Z9 4 U1 1 U2 6 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 EI 1475-2654 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD JUN PY 2015 VL 16 IS 1 BP 15 EP 26 DI 10.1017/S1466252315000122 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA CK1CV UT WOS:000355943900003 PM 26050568 ER PT J AU Ridpath, JF AF Ridpath, Julia F. TI Emerging pestiviruses infecting domestic and wildlife hosts SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Review DE emerging pestivirus; virulence variation; host expansion ID BOVINE-VIRAL-DIARRHEA; CHAMOIS RUPICAPRA-PYRENAICA; BORDER-DISEASE VIRUS; HOBI-LIKE PESTIVIRUS; NEW-WORLD CAMELIDS; PERSISTENT INFECTION; NORTH-AMERICA; SWINE-FEVER; CALVES; THROMBOCYTOPENIA AB Until the early 1990s there were just three recognized species in the pestivirus genus, bovine viral diarrhea virus (BVDV), border disease virus (BDV) and classical swine fever virus (CSFV). Subsequently BVDV were divided into two different species, BVDV1 and BVDV2 and four additional putative pestivirus species have been identified, based on phylogenetic analysis. The four putative pestivirus specices, listed in chronological order of published reports, are Giraffe (isolated from one of several giraffes in the Nanyuki District of Kenya suffering from mucosal disease-like symptoms), HoBi (first isolated from fetal bovine serum originating in Brazil and later from samples originating in Southeast Asia), Pronghorn (isolated from an emaciated blind pronghorn antelope in the USA), and Bungowannah (isolated following an outbreak in pigs, resulting in still birth and neonatal death, in Australia). In addition to the emergence of putative new species of pestivirus, changes in host and virulence of recognized or classic' pestiviruses have led to reevaluation of disease control programs and management of domestic and wildlife populations. C1 ARS, USDA, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Ridpath, JF (reprint author), ARS, USDA, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, POB 70,1920 Dayton Ave, Ames, IA 50010 USA. EM julia.ridpath@ars.usda.gov NR 46 TC 1 Z9 1 U1 5 U2 19 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 EI 1475-2654 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD JUN PY 2015 VL 16 IS 1 BP 55 EP 59 DI 10.1017/S1466252315000067 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CK1CV UT WOS:000355943900007 PM 26050572 ER PT J AU Bauermann, FV Ridpath, JF AF Bauermann, Fernando V. Ridpath, Julia F. TI HoBi-like viruses - the typical "atypical bovine pestivirus' SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Review DE HoBi-like viruses; atypical pestiviruses; bovine viral diarrhea virus; BVDV-3; clinical signs; diagnostic; epidemiology ID VIRAL DIARRHEA VIRUS; CALVES; INFECTION; IDENTIFICATION; DISEASE; SAMPLES; EUROPE; CATTLE; PIGS AB HoBi-like viruses, also referred to as bovine viral diarrhea virus 3 (BVDV-3) and atypical pestivirus, have been proposed as a new putative bovine pestivirus species. These viruses were first identified in the last decade and are currently distributed in at least three continents. Published findings suggest that these viruses may be endemic at least in parts of South America and Asia. The clinical presentations in cattle, described in field outbreaks and controlled studies, are similar to those associated with BVDV and range from subclinical to mild clinical signs, sporadically associated with reproductive losses, respiratory illness and hemorrhagic syndrome. The complete host range of HoBi-like virus is unknown, but data suggest higher adaptation of HoBi-like viruses to ruminants than swine. Acute infections, characterized by mild clinical signs, such as low-grade pyrexia and leukopenia, have been observed in both cattle and sheep. Virus has been isolated from nasal swabs indicating that virus was being shed. While seroconversion has been observed in pigs, no clinical presentation or viral shedding was evident following inoculation. While some commercial BVDV diagnostic tests may detect HoBi-like viruses, these tests do not differentiate between BVDV and HoBi-like viruses. The differentiation of BVDV and HoBi-like viruses is critical to the design of surveillance programs for these viruses. C1 [Bauermann, Fernando V.; Ridpath, Julia F.] ARS, USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. RP Bauermann, FV (reprint author), ARS, USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM Fernando.bauermann@ars.usda.gov NR 30 TC 1 Z9 3 U1 1 U2 4 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 EI 1475-2654 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD JUN PY 2015 VL 16 IS 1 BP 64 EP 69 DI 10.1017/S146625231500002X PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CK1CV UT WOS:000355943900009 PM 26050574 ER PT J AU Woodbury, BL Gilley, JE Parker, DB Marx, DB Eigenberg, RA AF Woodbury, Bryan L. Gilley, John E. Parker, David B. Marx, David B. Eigenberg, Roger A. TI VOC emissions from beef feedlot pen surfaces as affected by within-pen location, moisture and temperature SO BIOSYSTEMS ENGINEERING LA English DT Article DE Air quality; Feedlot; Gas chromatography; Odour; VOC ID WET DISTILLERS GRAINS; ANIMAL FEEDING OPERATIONS; GAS-CHROMATOGRAPHY-OLFACTOMETRY; INVERSE-DISPERSION TECHNIQUE; ORGANIC SULFUR-COMPOUNDS; CATTLE FEEDLOTS; ODORANT PRODUCTION; AMMONIA EMISSIONS; ODOROUS COMPOUNDS; ESCHERICHIA-COLI AB A laboratory study was conducted to evaluate the effects of pen location, moisture, and temperature on emissions of volatile organic compounds (VOC) from surface materials obtained from feedlot pens where beef cattle were fed a diet containing 30% wet distillers grain plus solubles. Surface materials were collected from the feed trough (bunk), drainage, and raised areas (mounds) within three feedlot pens. The surface materials were mixed with water to represent dry, wet, or saturated conditions and then incubated at temperatures of 5, 15, 25 and 35 degrees C. A wind tunnel and gas chromatograph-mass spectrometer were used to collect and quantify emissions of eight volatile fatty acids (VFAs), five aromatics and two sulfur-containing compounds. Pen location significantly (P <0.05) affected measurements of 10 of the VOC with the largest values occurring for materials collected near the mound area. The largest VFA and aromatic emissions resulted for the dry moisture condition while wet and saturated conditions produced the largest sulfide emissions. Temperature affected emission of each VOC except indole, with values generally increasing as temperature increased. Odour activity value (OAV), which was the ratio of measured concentration of a single compound normalised to the odour threshold for that compound, was calculated for each compound. Four VFAs contributed 7.5% of the total OAV but only one aromatic, 4-methylphenol, was a major contributor to total OAV at 2.5%. In comparison, sulfide compounds contributed 87.3% of the total OAV. This research shows VOC emissions are affected by pen location, moisture condition, and temperature. Published by Elsevier Ltd on behalf of IAgrE. C1 [Woodbury, Bryan L.; Eigenberg, Roger A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Gilley, John E.] USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Parker, David B.] West Texas A&M Univ, Canyon, TX 79016 USA. [Marx, David B.] Univ Nebraska, Lincoln, NE 68583 USA. RP Woodbury, BL (reprint author), USDA ARS, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM Bryan.Woodbury@ARS.USDA.Gov NR 61 TC 3 Z9 3 U1 3 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 EI 1537-5129 J9 BIOSYST ENG JI Biosyst. Eng. PD JUN PY 2015 VL 134 BP 31 EP 41 DI 10.1016/j.biosystemseng.2015.03.014 PG 11 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA CJ7VG UT WOS:000355707800004 ER PT J AU Barba, M Stewart, AJ Passler, T Hathcock, T Wooldridge, AA van Santen, E Chamorro, MF Cattley, RC Hogsette, JA Hu, XP AF Barba, Marta Stewart, Allison J. Passler, Thomas Hathcock, Terri Wooldridge, Anne A. van Santen, E. Chamorro, Manuel F. Cattley, Russell C. Hogsette, Jerome A. Hu, Xing Ping TI Experimental inoculation of house flies Musca domestica with Corynebacterium pseudotuberculosis biovar equi SO BULLETIN OF INSECTOLOGY LA English DT Article DE Horse; pigeon fever; vector; equine pathogen; arthropod; transmission ID HORSES; INFECTION AB Cotynebacterium pseudotuberculosis (Actinomycetales Corynebacteriaceae) infection in horses causes three different disease syndromes: external abscesses, infection of internal organs and ulcerative lymphangitis. The exact mechanism of infection in horses remains undetermined, but transmission by insect vectors is suspected. The present study first determined the optimal culture media for inoculation of house flies (Musca domestica L.) (Diptera Muscidae), with C. pseudotuberculosis biovar equi and the time required for fly inoculation. A second experiment determined the duration of bacterial survival on flies. Exposure of house flies to 3 different preparations of blood agar supplemented with dextrose and colonized with C. pseudotuberculosis determined that a 10 minute exposure to the bacteria was enough to inoculate the flies. C. pseudotuberculosis could be recovered for up to 24 hours after house flies were exposed for 30 minutes to a blood agar plate colonized with the bacteria and moistened with 10% dextrose. These findings support the hypothesis that the house fly is a potential vector of pigeon fever and aid in establishing a protocol for a future experimental model to demonstrate the role of house flies as mechanical vectors in C. pseudotuberculosis infection. C1 [Barba, Marta; Stewart, Allison J.; Passler, Thomas; Wooldridge, Anne A.; Chamorro, Manuel F.] Auburn Univ, Coll Vet Med, Dept Clin Sci, Auburn, AL 36849 USA. [Hathcock, Terri; Cattley, Russell C.] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA. [van Santen, E.] Auburn Univ, Coll Agr, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. [Hogsette, Jerome A.] Univ Florida, USDA ARS, CMAVE, Gainesville, FL 32611 USA. [Hu, Xing Ping] Auburn Univ, Coll Agr, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. RP Stewart, AJ (reprint author), Auburn Univ, Large Anim Teaching Hosp, Dept Clin Sci, 1500 Wire Rd, Auburn, AL 36849 USA. EM stewaaj@auburn.edu; huxingp@auburn.edu RI Stewart, Allison/E-4355-2016; Barba Recreo, Marta/I-1888-2016 OI Stewart, Allison/0000-0002-2464-3954; Barba Recreo, Marta/0000-0002-3677-7239 FU Boehringer Ingelheim Vetmedica, Inc.; Advancement of Equine Research Award; Auburn University College of Veterinary Medicine Animal Health and Disease Research Funds FX The authors thank Qiao Zhong for technical assistance. Financial support was provided by Boehringer Ingelheim Vetmedica, Inc. Advancement of Equine Research Award and Auburn University College of Veterinary Medicine Animal Health and Disease Research Funds. NR 20 TC 0 Z9 0 U1 1 U2 5 PU ALMA MATER STUDIORUM, UNIV BOLOGNA PI BOLOGNA PA DEPT AGROENVIRONMENTAL SCIENCES & TECHNOLOGY, VIALE G FANIN, 42, BOLOGNA, 40127, ITALY SN 1721-8861 J9 B INSECTOL JI Bull. Insectology PD JUN PY 2015 VL 68 IS 1 BP 39 EP 44 PG 6 WC Entomology SC Entomology GA CK1JN UT WOS:000355963700007 ER PT J AU Marcinkowski, K Peterson, DL Ettl, GJ AF Marcinkowski, Kailey Peterson, David L. Ettl, Gregory J. TI Nonstationary temporal response of mountain hemlock growth to climatic variability in the North Cascade Range, Washington, USA SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE dendrochronology; mountain hemlock; North Cascades; limiting factors; climate ID SUB-ALPINE FIR; TREE-GROWTH; PACIFIC-NORTHWEST; TSUGA-MERTENSIANA; OLYMPIC-MOUNTAINS; ABIES-LASIOCARPA; RADIAL GROWTH; NATIONAL-PARK; LODGEPOLE PINE; SIERRA-NEVADA AB A stationary response of tree radial growth to climatic variables is assumed as a basis for climatic reconstructions and future growth projections in response to climate change. Mountain hemlock (Tsuga mertensiana (Bong.) Carriere) trees on the western slopes of the North Cascade Range (Washington, USA) were examined for stability in growth response to climatic influences at a small spatial scale. Moving correlation functions demonstrate that climate-growth interactions are nonstationary over time, alternating between periods of significant and nonsignificant responses. Correlations between growth and winter precipitation have weakened, becoming statistically insignificant in the last decade, but correlations with spring temperature and previous-year summer temperature have strengthened, becoming statistically significant. The Pacific Decadal Oscillation influences patterns in climate-growth correlations but does not seem to account for the most recent changes in correlation strength. At an interannual scale, growth differs between El Nino Southern Oscillation phases, specifically between El Nino and La Nina years and between La Nina and neutral phase years. The variability in growth response to climate at interannual and interdecadal time frames, especially with the climate changes emerging in recent decades, will challenge the reliability and accuracy of reconstruction and predictive models. C1 [Marcinkowski, Kailey; Ettl, Gregory J.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Peterson, David L.] US Forest Serv, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Marcinkowski, K (reprint author), Northern Inst Appl Climate Sci, Forest Sci Lab, 410 Macinnes Dr, Houghton, MI 49931 USA. EM kfmarcin@mtu.edu FU U.S. Geological Survey Global Change Research Program; University of Washington School of Environmental and Forest Sciences FX We thank Maureen Kennedy for providing valuable statistical advice and helpful discussions during the research process. Maureen Kennedy and Don McKenzie reviewed the initial manuscript and provided helpful suggestions. Joe Restaino, Jon Dvorak, Jon McDuffy, Jim Cronan, Brandon Helmstetter, and Robert Norheim assisted with fieldwork. Robert Norheim produced Fig. 1. Anonymous reviewers provided comments and suggestions that strengthened our paper, and we are grateful for their contributions. Research was funded by the U.S. Geological Survey Global Change Research Program and the University of Washington School of Environmental and Forest Sciences. This paper is a contribution of the Western Mountain Initiative. NR 60 TC 4 Z9 4 U1 5 U2 23 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JUN PY 2015 VL 45 IS 6 BP 676 EP 688 DI 10.1139/cjfr-2014-0231 PG 13 WC Forestry SC Forestry GA CJ5TR UT WOS:000355553400009 ER PT J AU Wear, DN Dixon, E Abt, RC Singh, N AF Wear, David N. Dixon, Ernest Abt, Robert C. Singh, Navinder TI Projecting Potential Adoption of Genetically Engineered Freeze-Tolerant Eucalyptus in the United States SO FOREST SCIENCE LA English DT Article DE real options; investment analysis; genetically modified organism (GMO); bioenergy ID ENERGY CROPS; UNCERTAINTY; US AB Development of commercial Eucalyptus plantations has been limited in the United States because of the species' sensitivity to freezing temperatures. Recently developed genetically engineered clones of a Eucalyptus hybrid, which confer freeze tolerance, could expand the range of commercial plantations. This study explores how freeze-tolerant Eucalyptus might be adopted as a preferred land use based on comparative returns and a real options land-use switching model. Climate factors other than freezing are assumed to limit potential adoption to the southeastern region of the United States. Comparison of returns indicates that Eucalyptus would probably not compete with cropland but could be competitive with forest uses, especially planted pine. Real options analysis, using both geometric Brownian motion and mean reverting models of stochastic returns, indicates that switching could be expected on a portion of planted pine forestland. Models predict about 0.8-1.4 million acres of Eucalyptus plantations (5-9% of the current area of planted pine). Extending the analysis to also consider the current area of naturally regenerated pine results in as much as 2.8 million acres of Eucalyptus. Actual adoption will probably depend on uncertain future markets for cellulose, especially for bioenergy feedstock. C1 [Wear, David N.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC 27709 USA. [Dixon, Ernest; Abt, Robert C.; Singh, Navinder] N Carolina State Univ, Raleigh, NC 27695 USA. RP Wear, DN (reprint author), US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC 27709 USA. EM dwear@fs.fed.us; ebdixon4@gmail.com; bob_abt@ncsu.edu; nindz27@gmail.com NR 21 TC 2 Z9 2 U1 4 U2 16 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2015 VL 61 IS 3 BP 466 EP 480 DI 10.5849/forsci.14-089 PG 15 WC Forestry SC Forestry GA CJ7ZA UT WOS:000355717700008 ER PT J AU Niblett, MR Sweeney, SH Church, RL Barber, KH AF Niblett, Matthew R. Sweeney, Stuart H. Church, Richard L. Barber, Klaus H. TI Structure of Fisher (Pekania pennanti) Habitat in a Managed Forest in an Interior Northern California Coast Range SO FOREST SCIENCE LA English DT Article DE habitat characterization; spatial analysis; industrial forest management; habitat analysis ID RESOURCE SELECTION; MARTES-PENNANTI; INVENTORY DATA; SUITABILITY; REGION; MODELS; RISKS AB Forest management planning across the Sierra Nevada and Northern California includes several focal wildlife species, one of which is the fisher, recently reclassified as Pekania pennant! (Sato et al. 2012) from Martes pennanti. This article presents an analysis of fisher habitat associated with privately owned forestland in Northern California. We take advantage of a high-resolution inventory database to show selective use of female fishers within this landscape. This landscape can be thought of as a matrix containing scattered mature stands of conifers and hardwoods (age >100 years) within a landscape of younger mixed conifer stands and numerous openings (24% of the area). Although stand and forest averages for habitat elements appear to score poorly using a widely used metric, we demonstrate through a new form of spatial sampling using a Kolmogorov-Smirnov (K-S) statistical test, which we call k-max, selective habitat use by female fishers at a relatively fine scale. This approach is novel in terms of generating population-level inferential results that are sensitive to smaller scale selection behavior, demonstrating that existing large structures, even though limited in areal extent, are critical elements in female fisher habitat. C1 [Niblett, Matthew R.] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Sweeney, Stuart H.; Church, Richard L.] Univ Calif Santa Barbara, Santa Barbara, CA 93106 USA. [Barber, Klaus H.] US Forest Serv, USDA, Reg Analyst Ecosyst Planning, Res Triangle Pk, NC 27709 USA. RP Niblett, MR (reprint author), Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. EM mniblett@geog.ucsb.edu; sweeney@geog.ucsb.edu; church@geog.ucsb.edu; 4khbarber@gmail.com NR 38 TC 0 Z9 0 U1 4 U2 17 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2015 VL 61 IS 3 BP 481 EP 493 DI 10.5849/forsci.13-086 PG 13 WC Forestry SC Forestry GA CJ7ZA UT WOS:000355717700009 ER PT J AU Vose, JM Miniat, CF Sun, G Caldwell, PV AF Vose, James M. Miniat, Chelcy F. Sun, Ge Caldwell, Peter V. TI Potential Implications for Expansion of Freeze-Tolerant Eucalyptus Plantations on Water Resources in the Southern United States SO FOREST SCIENCE LA English DT Article DE evapotranspiration; streamflow; modeling; water budget ID CATCHMENT-SCALE; FORESTED WATERSHEDS; ENERGY-EXCHANGE; CLIMATE-CHANGE; EVAPOTRANSPIRATION; PINE; HYDROLOGY; BALANCE; FLORIDA; BRAZIL AB The potential expansion of freeze-tolerant (FT) Eucalyptus plantations in the United States has raised concerns about the implications for water resources. Modeling was used to examine the potential effects of expanding the distribution of FT Eucalyptus plantations in US Department of Agriculture Plant Hardiness Zones 8b and greater on water yield (Q). Analyses focused on two scales: the stand scale and the regional scale at the 12-digit Hydrologic Unit Code (HUC) watershed. Results suggested that the stand-level implications of planting FT Eucalyptus on Q could vary by location, the land cover type before Eucalyptus establishment, and the hydrologic conditions of the planting site and surrounding area. Compared with that for some pine plantations, Q at the stand level could be equal to or reduced by as much as to 130 mm year(-1) (a reduction of 9-16% of precipitation) near the end of the rotation or on sites when leaf area index (LAI) is 4 m(2) m(-2) and reduced by as much as 500 mm year(-1) (a reduction of 33-63% of precipitation) when LAI is 5 m(2) m(-2). In contrast, at the scale of conversion indicated by an economic analysis as most likely (e.g., <20% conversion of conifer to FT eucalyptus), reductions on Q at the 12-digit HUC scale will be negligible. C1 [Vose, James M.] US Forest Serv, USDA, Ctr Integrated Forest Sci, Raleigh, NC 27699 USA. [Miniat, Chelcy F.; Caldwell, Peter V.] US Forest Serv, USDA, Coweeta Hydrol Lab, Otto, NC USA. [Sun, Ge] US Forest Serv, USDA, Eastern Forest Threats Ctr, Raleigh, NC USA. RP Vose, JM (reprint author), US Forest Serv, USDA, Ctr Integrated Forest Sci, Raleigh, NC 27699 USA. EM cfminiat@fs.fed.us; gesun@fs.fed.us; pcaldwell02@fs.fed.us OI Miniat, Chelcy/0000-0002-3266-9783 NR 49 TC 2 Z9 2 U1 6 U2 18 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2015 VL 61 IS 3 BP 509 EP 521 DI 10.5849/forsci.14-087 PG 13 WC Forestry SC Forestry GA CJ7ZA UT WOS:000355717700011 ER PT J AU Blazier, MA Clason, TR Liechty, HO Leggett, ZH Sucre, EB Roberts, SD Krapfl, K Vance, ED AF Blazier, Michael A. Clason, Terry R. Liechty, Hal O. Leggett, Zakiya H. Sucre, Eric B. Roberts, Scott D. Krapfl, Kurt Vance, Eric D. TI Nitrogen and Carbon of Switchgrass, Loblolly Pine, and Cottonwood Biofuel Production Systems in the Southeast United States SO FOREST SCIENCE LA English DT Article DE nitrogen; carbon; bioenergy; agroforestry ID SOIL MICROBIAL COMMUNITY; ORGANIC-MATTER; BIOCIDAL TREATMENTS; HARVEST FREQUENCY; FORESTS; AGRICULTURE; FUMIGATION; METABOLISM; BIOMASS; NITRATE AB Switchgrass has favorable characteristics as a biofuel feedstock, and it may be feasibly grown on sites currently forested or retired from agricultural production. It is ecologically important to understand the impacts of establishing and managing switchgrass on site nutrient cycling. Four studies conducted in the southeast United States tested switchgrass as a bioenergy crop grown in monoculture, in alley cropping systems, and as an alternative to conventional agricultural rotations. Each trial included comparisons of switchgrass with the prevailing land uses. In each trial, nitrogen mineralization (N-min), nitrification (N-nit), microbial biomass carbon (C-mic), microbial activity, and labile carbon were measured because of their sensitivity to management practices that alter soil nutrient cycling. In all studies of loblolly pine and switchgrass, switchgrass was apparently the dominant substrate source for (C-mic, and N-min increased when juvenile loblolly pine was isolated with herbicide as the sole vegetation. However, C-mic and N-min, of loblolly pine-switchgrass alley cropping systems were similar to those observed in conventional juvenile labially pine plantation conditions. In a trial of converting former agricultural fields into switchgrass or cottonwood biofuel plantations, this conversion reduced N-nit, and therefore the potential for nitrogen losses compared with those for a soybean-sorghum rotation typical for such sites. C1 [Blazier, Michael A.] Louisiana State Univ, Ctr Agr, Hill Farm Res Stn, Homer, LA 71040 USA. [Clason, Terry R.] USDA, Nat Resource Conservat Serv, Washington, DC USA. [Liechty, Hal O.] Univ Arkansas Monticello, Monticello, AR 71655 USA. [Roberts, Scott D.; Krapfl, Kurt] Mississippi State Univ, Mississippi State, MS 39762 USA. RP Blazier, MA (reprint author), Louisiana State Univ, Ctr Agr, Hill Farm Res Stn, Homer, LA 71040 USA. EM mblazier@agcenter.lsu.edu; clasont@bellsouth.net; liechty@uamont.edu; zakiya.leggett@weyerhaeuser.com; eric.sucre@weyerhaeuser.com; sroberts@cfr.msstate.edu; kkrapfl@cfr.msstate.edu; evance@ncasi.org FU National Council of Air and Stream Improvement, Catchlight Energy, Sun Grant; USDA Natural Resource Conservation Service FX The authors gratefully acknowledge the National Council of Air and Stream Improvement, Catchlight Energy, Sun Grant, and the USDA Natural Resource Conservation Service for the funding that made this research possible. NR 44 TC 2 Z9 2 U1 1 U2 19 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2015 VL 61 IS 3 BP 522 EP 534 DI 10.5849/forsci.14-016 PG 13 WC Forestry SC Forestry GA CJ7ZA UT WOS:000355717700012 ER PT J AU Jain, TB Graham, RT AF Jain, Theresa B. Graham, Russell T. TI Decrease in Sapling Nutrient Concentrations for Six Northern Rocky Mountain Coniferous Species SO FOREST SCIENCE LA English DT Article DE soil productivity; young forests; small tree utilization; cleanings and weedings; precommercial thinning ID PINUS-MONTICOLA; THINNING STANDS; UNITED-STATES; ENERGY WOOD; FOREST; BIOMASS; GROWTH; CARBON; DECOMPOSITION; MANAGEMENT AB In the west, fire exclusion, timber harvest, and last century's climate led to copious regeneration on millions of ha that now need tending. Without treatment, overcrowding increases competition, snow and ice damage potential, and ladder fuels. Limited funding prevents treating all of the affected ha, but by selling small trees for wood pellets, biofuel, or methanol, costs would decrease and more ha could be cleaned. In the northern Rocky Mountains, 8-10 tree species can occur in young forests, and until this study, information on foliage nutrient concentrations and leaching from saplings for all of these species was limited. We use a mixed-model analysis to examine the change in nutrient concentrations (calcium, magnesium, potassium, carbon, and nitrogen) remaining in saplings for 6 tree species (western white pine, ponderosa pine, western hemlock, western redcedar, Douglas-fir, and grand fir) over a 12-month period. Nutrient concentrations and the pattern of nutrient leaching did vary among the species, but we found that nutrient concentrations did not diminish in the first 200 days and western hemlock was the only species for which nutrient concentrations leached substantially in the 12-month period. Results suggest managers may need to use biomass utilization thresholds to ensure sufficient biomass is left on site to maintain soil productivity. C1 [Jain, Theresa B.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Graham, Russell T.] US Forest Serv, USDA, Washington, DC USA. RP Jain, TB (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. EM tjain@fs.fed.us; rtgraham@fs.fed.us FU USDA Forest Service; Rocky Mountain Research Station; National Fire Plan FX We thank Joanne Tirocke, Jonathan Sandquist, Don Shipton, and Gail Fullerton for conducting the sampling and laboratory analysis; there were many samples that needed processing and without these people doing all the leg work, this study would not have been possible. The associate editor and reviewers provided excellent comments and nuances that were helpful in our revisions. This study was supported by the USDA Forest Service, Rocky Mountain Research Station and funded through the National Fire Plan. NR 52 TC 0 Z9 0 U1 4 U2 7 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2015 VL 61 IS 3 BP 570 EP 578 DI 10.5849/forsci.13-174 PG 9 WC Forestry SC Forestry GA CJ7ZA UT WOS:000355717700018 ER PT J AU Briske, DD Joyce, LA Polley, HW Brown, JR Wolter, K Morgan, JA McCarl, BA Bailey, DW AF Briske, David D. Joyce, Linda A. Polley, H. Wayne Brown, Joel R. Wolter, Klaus Morgan, Jack A. McCarl, Bruce A. Bailey, Derek W. TI Climate-change adaptation on rangelands: linking regional exposure with diverse adaptive capacity SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID UNITED-STATES; AUSTRALIAN RANGELANDS; SHORTGRASS STEPPE; PRECIPITATION; VARIABILITY; STRATEGIES; INCREASES; CO2; VULNERABILITY; CONSEQUENCES AB The ecological consequences of climate change are predicted to vary greatly throughout US rangelands. Projections show warming and drying in the southern Great Plains and the Southwest, warmer and drier summers with reduced winter snowpack in the Northwest, and warmer and wetter conditions in the northern Great Plains. Primarily through their combined effects on soil water availability, these climatic changes will modify plant production and community composition, which will, in turn, affect the livelihoods of humans who rely upon livestock grazing. The ability of rangeland managers to assess risk and prepare for climate change varies greatly and reflects their different adaptive capacities. Geographically specific exposure to climate change and a diverse adaptive capacity to counteract these changes will require development of varied adaptation strategies that can accommodate the various needs and abilities of livestock managers. C1 [Briske, David D.] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [Joyce, Linda A.] US Forest Serv, Human Dimens Res Program, USDA, Rocky Mt Res Stn, Ft Collins, CO USA. [Polley, H. Wayne] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Brown, Joel R.] New Mexico State Univ, USDA, Jornada Expt Range, Nat Resource Conservat Serv, Las Cruces, NM 88003 USA. [Wolter, Klaus] Univ Colorado, Boulder, CO 80309 USA. [Wolter, Klaus] Natl Ocean & Atmospher Adm, Earth Syst Res Lab, Boulder, CO USA. [Morgan, Jack A.] USDA ARS, Crops Res Lab, Ft Collins, CO 80526 USA. [McCarl, Bruce A.] Texas A&M Univ, Dept Agr Econ, College Stn, TX 77843 USA. [Bailey, Derek W.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. RP Briske, DD (reprint author), Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. EM dbriske@tamu.edu NR 40 TC 5 Z9 5 U1 5 U2 43 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD JUN PY 2015 VL 13 IS 5 BP 249 EP 256 DI 10.1890/140266 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ6NK UT WOS:000355610300007 ER PT J AU Goldman, JJ AF Goldman, Jason J. TI DNA contents in Texas bluegrass (Poa arachnifera) selected in Texas and Oklahoma determined by flow cytometry SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE DNA content; Flow cytometry; Poa arachnifera; Texas bluegrass ID EASTERN GAMAGRASS; REGISTRATION AB Texas bluegrass (Poa arachnifera Torr.) is a dioecious, perennial, cool-season grass native to southern Kansas, Oklahoma, western Arkansas and most of Texas. Its major use has been for forage on rangelands in Texas and Oklahoma. More recently, interspecific hybrids between Texas bluegrass and Kentucky bluegrass (P. pratensis L.) have been developed and commercially released as heat tolerant turfgrass. Currently, the 2C nuclear DNA content of Texas bluegrass is unknown. Therefore, flow cytometry was performed on an assortment of male and female Texas bluegrass genotypes (n = 24) collected from fourteen locations in Texas and Oklahoma to estimate DNA content in picograms (pg). The DNA content of Texas bluegrass fell into two groups based on collection location. Plants collected from northwest Oklahoma and five other locations west of 33.75, -98.39 (latitude, longitude) ranged from 9.64 +/- A 0.04 to 10.07 +/- A 0.09 pg. Plants collected from four sites in Texas, east of 33.20, -98.16 contained a DNA content that ranged from 14.02 +/- A 0.04 to 14.75 +/- A 0.13 pg. Based on a root tip chromosome count, a male Texas bluegrass plant estimated to be 9.96 pg, appeared to contain 56 chromosomes, indicating the smaller DNA content plants may be octaploids (2n = 8x = 56). Significant differences were not detected between male and female plants based on DNA content. Knowledge of the DNA content in Texas bluegrass should be useful in a breeding program for characterizing germplasm and identifying desired DNA content levels among parental genotypes. C1 USDA ARS, Southern Plains Range Res Stn, Woodward, OK 73801 USA. RP Goldman, JJ (reprint author), USDA ARS, Southern Plains Range Res Stn, 2000 18th St, Woodward, OK 73801 USA. EM jason.goldman@ars.usda.gov NR 16 TC 1 Z9 2 U1 4 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD JUN PY 2015 VL 62 IS 5 BP 643 EP 647 DI 10.1007/s10722-015-0247-1 PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CJ9DC UT WOS:000355801600002 ER PT J AU Volk, GM Chao, CT Norelli, J Brown, SK Fazio, G Peace, C McFerson, J Zhong, GY Bretting, P AF Volk, Gayle M. Chao, C. Thomas Norelli, Jay Brown, Susan K. Fazio, Gennaro Peace, Cameron McFerson, Jim Zhong, Gan-Yuan Bretting, Peter TI The vulnerability of US apple (Malus) genetic resources SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Breeding; Disease resistance; Genetic diversity; Germplasm collections; Malus; Wild species ID FIRE BLIGHT RESISTANCE; X-DOMESTICA BORKH.; CROP WILD RELATIVES; REPLANT DISEASE; MOLECULAR MARKERS; GERMPLASM COLLECTION; CORE COLLECTION; LIQUID-NITROGEN; CLIMATE-CHANGE; CENTRAL-ASIA AB Apple (Malus x domestica Borkh.) is one of the top three US fruit crops in production and value. Apple production has high costs for land, labor and inputs, and orchards are a long-term commitment. Production is dominated by only a few apple scion and rootstock cultivars, which increases its susceptibility to dynamic external threats. Apple crop wild relatives, including progenitor species Malus sieversii (Ledeb.) M. Roem., Malus orientalis Uglitzk., Malus sylvestris (L.) Mill., and Malus prunifolia (Willd.) Borkh., as well as many other readily hybridized species, have a wide range of biotic and abiotic stress resistances as well as desirable productivity and fruit quality attributes. However, access to wild materials is limited and wild Malus throughout the world is at risk of loss due to human encroachment and changing climatic patterns. The USDA-ARS National Plant Germplasm System (NPGS) Malus collection, maintained by the Plant Genetic Resources Unit in Geneva, NY, US is among the largest collections of cultivated apple and Malus species in the world. The collection currently has 5004 unique accessions in the field and 1603 seed accessions representing M. x domestica, 33 Malus species, and 15 hybrid species. Of the trees in the field, 3,070 are grafted and are represented by a core collection of 258 individuals. Many wild species accessions are represented as single seedlings (non-grafted). The crop vulnerability status of apple in the US is moderate because although there are a few breeders developing new commercial cultivars who also access Malus species, threats and challenges include new diseases, pests, and changing climate combined with industry needs and consumer demands, with a limited number of cultivars in production. C1 [Volk, Gayle M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Chao, C. Thomas; Fazio, Gennaro; Zhong, Gan-Yuan] USDA ARS, Plant Genet Resources Unit, Geneva, NY 14456 USA. [Norelli, Jay] USDA ARS, Appalachian Fruit Res Lab, Kearneysville, WV 25430 USA. [Brown, Susan K.] New York State Agr Exptl Stn, Dept Hort, Geneva, NY 14456 USA. [Peace, Cameron] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [McFerson, Jim] Washington Tree Fruit Res Commiss, Wenatchee, WA 98801 USA. [Bretting, Peter] USDA ARS, George Washington Carver Ctr, Beltsville, MD USA. RP Volk, GM (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM Gayle.Volk@ars.usda.gov NR 170 TC 2 Z9 2 U1 4 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD JUN PY 2015 VL 62 IS 5 BP 765 EP 794 DI 10.1007/s10722-014-0194-2 PG 30 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CJ9DC UT WOS:000355801600010 ER PT J AU Anthanont, P Asztalos, BF Polisecki, E Zachariah, B Schaefer, EJ AF Anthanont, Pimjai Asztalos, Bela F. Polisecki, Eliana Zachariah, Benoy Schaefer, Ernst J. TI Case report: A novel apolipoprotein A-I missense mutation apoA-I (Arg149Ser)(Boston) associated with decreased lecithin-cholesterol acyltransferase activation and cellular cholesterol efflux SO JOURNAL OF CLINICAL LIPIDOLOGY LA English DT Editorial Material DE HDL deficiency; Apolipoprotein A-I; Genetic mutation; Cellular cholesterol efflux; LCAT activity; CETP activity ID CORONARY-HEART-DISEASE; DENSITY-LIPOPROTEIN CHOLESTEROL; OF-THE-LITERATURE; LCAT DEFICIENCY; HDL DEFICIENCY; CAPACITY; HETEROZYGOSITY; VARIANTS; RESIDUES; PLASMA AB We report a novel heterozygous apolipoprotein A-I (apoA-I) missense mutation (c.517C>A, p.Arg149Ser, designated as apoA-I-Boston) in a 67-year-old woman and her 2 sons, who had mean serum high-density lipoprotein (HDL) cholesterol, apoA-I, and apoA-I in very large alpha-1 HDL that were 10%, 35%, and 16% of normal, respectively (all P < .05). The percentage of HDL cholesterol in the esterified form was also significantly (P < .05) reduced to 52% of control values. Cholesteryl ester tranfer protein (CETP) activity was normal. The mean global, adenosine triphosphate (ATP)-binding cassette transporter A1 and scavenger receptor B type I mediated cellular cholesterol efflux capacity in apoB-depleted serum from affected family members were 41%, 37%, 47%, 54%, and 48% of control values, respectively (all P < .05). lecithin-cholesterol acyltransferase (LCAT) activity in plasma was 71% of controls, whereas in the cell-based assay, it was 73% of control values (P < .05). The data indicate that this novel apoA-I missense is associated with markedly decreased levels of HDL cholesterol and very large alpha-1 HDL, as well as decreased serum cellular cholesterol efflux and LCAT activity, but not with premature coronary heart disease, similar to other apoA-I mutations that have been associated with decreased LCAT activity. (C) 2015 National Lipid Association. All rights reserved. C1 [Anthanont, Pimjai; Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Cardiovasc Nutr Lab, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Asztalos, Bela F.; Polisecki, Eliana; Schaefer, Ernst J.] Boston Heart Diagnost, Framingham, MA USA. [Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Zachariah, Benoy] Steward Hlth Good Samaritan Cardiol Grp, Brockton, MA USA. RP Schaefer, EJ (reprint author), Tufts Univ, Cardiovasc Nutr Lab, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Ernst.Schaefer@tufts.edu FU NHLBI NIH HHS [P50 HL083813, P50 HL083813-01] NR 21 TC 0 Z9 1 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1933-2874 EI 1876-4789 J9 J CLIN LIPIDOL JI J. Clin. Lipidol. PD JUN PY 2015 VL 9 IS 3 BP 390 EP 395 DI 10.1016/j.jacl.2015.02.005 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA CJ8UK UT WOS:000355778800017 PM 26073399 ER PT J AU Huang, JW Luo, YG Nou, XW AF Huang, Jingwei Luo, Yaguang Nou, Xiangwu TI Growth of Salmonella enterica and Listeria monocytogenes on Fresh-Cut Cantaloupe under Different Temperature Abuse Scenarios SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157H7; STORAGE-TEMPERATURE; NATIVE MICROFLORA; MELONS; CONTAMINATION; SURFACE; MICROORGANISMS; TYPHIMURIUM; INHIBITION; PATHOGENS AB Effective cold chain management is a critical component of food safety practice. In this study, we examined the impact of commonly encountered temperature abuse scenarios on the proliferation of Salmonella enterica and Listeria monocyto genes on fresh-cut cantaloupe. Inoculated fresh-cut cantaloupe cubes were subjected to various temperature abuse conditions, and the growth of S. enterica and L. monocyto genes was determined. During 1 week of storage, Salmonella cell counts on fresh-cut cantaloupe increased by -0.26, 1.39, and 2.23 log units at 4 degrees C (control), 8 degrees C, and 12 degrees C (chronic temperature abuse), respectively, whereas that of L. monocytogenes increased by 0.75, 2.86, and 4.17 log units. Under intermittent temperature abuse conditions, where storage temperature fluctuated twice daily to room temperature for 30 min, Salmonella cell count increased by 2.18 log units, whereas that of L. monocytogenes increased by 1.86 log units. In contrast, terminal acute temperature abuses for 2 to 4 h resulted in upwards to 0.6 log unit for Salmonella, whereas the effect on L. monocyto genes was less significant compared with L. monocytogenes on cut cantaloupe stored at 4 degrees C. Significant deterioration of produce visual quality and tissue integrity, as reflected by electrolyte leakage, was also observed under various temperature abuse conditions. C1 [Huang, Jingwei] Sichuan Agr Univ, Yaan 625014, Sichuan, Peoples R China. [Luo, Yaguang; Nou, Xiangwu] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. RP Nou, XW (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. EM xiangwu.nou@ars.usda.gov FU U.S. Department of Agriculture, National Institute of Food and Agriculture [2010-51181-21230]; Chinese National Scholarship Council (Beijing, China) FX The authors thank Dr. Eunhee Park for assistance with statistic analyses. This research is partially supported by U.S. Department of Agriculture, National Institute of Food and Agriculture (Specialty Crop Research Initiative award 2010-51181-21230). J. Huang is a predoctoral visiting scholar at U.S. Department of Agriculture, Agricultural Research Service, from Sichuan Agriculture University (Ya'an, China), sponsored by the Chinese National Scholarship Council (Beijing, China). NR 29 TC 5 Z9 5 U1 1 U2 10 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2015 VL 78 IS 6 BP 1125 EP 1131 DI 10.4315/0362-028X.JFP-14-468 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CJ8UW UT WOS:000355780000009 PM 26038902 ER PT J AU Hwang, CA Huang, LH Juneja, V AF Hwang, Cheng-An Huang, Lihan Juneja, Vijay TI Effect of Acidified Sorbate Solutions on the Lag-Phase Durations and Growth Rates of Listeria monocytogenes on Meat Surfaces SO JOURNAL OF FOOD PROTECTION LA English DT Article ID TO-EAT FOODS; POTASSIUM SORBATE; ORGANIC-ACIDS; SODIUM DIACETATE; VACUUM PACKAGES; UNITED-STATES; ENHANCED INHIBITION; POULTRY PRODUCTS; BEEF BOLOGNA; LACTATE AB The surfaces of ready-to-eat meats are susceptible to postprocessing contamination by Listeria monocyto genes. This study quantified the lag-phase durations (LPD) and growth rates (GR) of L. monocyto genes on the surfaces of cooked ham as affected by sorbate solutions of different concentrations and pH levels. Slices of cooked ham inoculated with a four-strain mixture of L. monocytogenes (ca. 10(3) CFU/g) were surface treated with sorbate solutions of 0 to 4% (wt/vol) at pH 4.0 to 6.5, vacuum packaged, and stored at 4 to 12 degrees C for up to 45 days. The LPD and GR of L. monocyto genes were used to develop response surface models. The models estimated that the LPD of L. monocytogenes in samples treated with solutions of pH 4.0 to 5.5 (no sorbate) were 0 to 11 days and the GR were 0.25 to 0.36 log CPU/day, respectively, at 4 degrees C. With the treatments of 2 and 4% (wt/vol) sorbate solutions, the LPD were estimated to be extended to 2 to 26 days and 34 to >45 days, and the GR were reduced to 0.15 to 0.30 and 0 to 0.19 log CPU/day, respectively. At 4 degrees C, increasing sorbate concentrations by 1% (wt/vol) to 2, 3, and 4% (wt/vol) at pH 5.5 to 4.0 led to an extension of LPD by 2 to 11, 10 to 19, and 18 to 27 days, whereas the GR were reduced by 0.037 to 0.055, 0.048 to 0.066, and 0.060 to 0.078 log CPU/day, respectively. Sorbate also extended the LPD and reduced the GR of L. monocytogenes at 8 and 12 degrees C. Results indicated that sorbate concentration and pH level were significant factors affecting the LPD and GR of L. monocytogenes and that the combination of sorbate and low pH has potential for use as a surface treatment to control L. monocyto genes on meat surfaces. C1 [Hwang, Cheng-An; Huang, Lihan; Juneja, Vijay] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Hwang, CA (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM andy.hwang@ars.usda.gov NR 37 TC 0 Z9 0 U1 2 U2 8 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2015 VL 78 IS 6 BP 1154 EP 1160 DI 10.4315/0362-028X.JFP-14-408 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CJ8UW UT WOS:000355780000013 PM 26038906 ER PT J AU Karns, JS Haley, BJ Van Kessel, JAS AF Karns, Jeffrey S. Haley, Bradd J. Van Kessel, Jo Ann S. TI Improvements to a PCR-Based Serogrouping Scheme for Salmonella enterica from Dairy Farm Samples SO JOURNAL OF FOOD PROTECTION LA English DT Article ID CERRO; HERD AB Molecular serotyping through the use of PCR is a simple and useful technique for characterizing isolates of Salmonella enterica subsp. enterica belonging to serogroups B, C1 , C2, D1, and El, which are the majority of the isolates associated with human disease outbreaks. However, many of the Salmonella strains currently isolated from dairy farms in the northeastern United States are serovar Cello, a group K strain not detected by this assay. Primers from a well-known PCR assay for the identification of Salmonella were added to a commonly used serotyping assay so that strains, such as Salmonella Cerro, that do not produce bands in the original assay can be confirmed as belonging to S. enterica subsp. enterica. The modified assay frequently misidentified the serogroup of Salmonella Mbandaka isolates because of failure to amplify the wzxC1 amplicon. Therefore, the reverse primer for the wzxC1 target was modified based on in silico analysis to provide consistent classification of Salmonella Mbandaka as belonging to serogroup C1. These two modifications to the serogrouping PCR method enhance the utility of the method for characterizing Salmonella isolates. C1 [Karns, Jeffrey S.; Haley, Bradd J.; Van Kessel, Jo Ann S.] ARS, USDA, Henry A Wallace Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Karns, JS (reprint author), ARS, USDA, Henry A Wallace Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM jeffrey.karns@ars.usda.gov NR 7 TC 2 Z9 2 U1 0 U2 3 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2015 VL 78 IS 6 BP 1182 EP 1185 DI 10.4315/0362-028X.JFP-14-475 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CJ8UW UT WOS:000355780000017 PM 26038910 ER PT J AU Novotny, JA Baer, DJ Khoo, C Gebauer, SK Charrons, CS AF Novotny, Janet A. Baer, David J. Khoo, Christina Gebauer, Sarah K. Charrons, Craig S. TI Cranberry Juice Consumption Lowers Markers of Cardiometabolic Risk, Including Blood Pressure and Circulating C-Reactive Protein, Triglyceride, and Glucose Concentrations in Adults SO JOURNAL OF NUTRITION LA English DT Article DE polyphenol; flavonoid; cardiovascular disease; blood lipids; diabetes; inflammation ID ANGIOTENSIN-CONVERTING ENZYME; SPONTANEOUSLY HYPERTENSIVE-RATS; CORONARY-HEART-DISEASE; GRAPE-SEED PROCYANIDINS; LOW-CALORIE SWEETENERS; HEPATOCYTE APO-B; HIGH-FAT DIET; FLAVONOID QUERCETIN; CARDIOVASCULAR-DISEASE; NITRIC-OXIDE AB Background: Cardiometabolic risk is the risk of cardiovascular disease (CVD), diabetes, or stroke, which are leading causes of mortality and morbidity worldwide. Objective: The objective of this study was to determine the potential of low-calorie cranberry juice (LCCJ) to lower cardiometabolic risk. Methods.: A double-blind, placebo-controlled, parallel-arm study was conducted with controlled diets. Thirty women and 26 men (mean baseline characteristics: 50 y; weight, 79 kg; body mass index, 28 kg/m(2)) completed an 8-wk intervention with LCCJ or a flavor/color/energy-matched placebo beverage. Twice daily volunteers consumed 240 mL of LCCJ or the placebo beverage, containing 173 or 62 mg of phenolic compounds and 6.5 or 7.5 g of total sugar per 240-mL serving, respectively. Results: Fasting serum triglycerides (TGs) were lower after consuming LCCJ and demonstrated a treatment x baseline interaction such that the participants with higher baseline TG concentrations were more likely to experience a larger treatment effect (1.15 +/- 0.04 mmol/L vs. 1.25 +/- 0.04 mmol/L, respectively; P = 0.027). Serum C-reactive protein (CRP) was lower for individuals consuming LCCJ than for individuals consuming the placebo beverage [In transformed values of 0.522 +/- 0.115 In(mg/L) vs. 0.997 +/- 0.120 In(mg/L), P = 0.0054, respectively, and equivalent to 1,69 mg/L vs. 2.71 mg/L back-transformed]. LCCJ lowered diastolic blood pressure (BP) compared with the placebo beverage (69.2 +/- 0.8 mm Hg for LCCJ vs. 71.6 +/- 0.8 mm Hg for placebo; P = 0.048). Fasting plasma glucose was lower (P = 0.03) in the LCCJ group (5.32 +/- 0.03 mmol/L) than in the placebo group (5.42 +/- .03 mmol/L), and LCCJ had a beneficial effect on homeostasis model assessment of insulin resistance for participants with high baseline values (P = 0.035). Conclusion: LCCJ can improve several risk factors of CVD in adults, including circulating TGs, CRP, and glucose, insulin resistance, and diastolic BP. This trial was registered at clinicaltrials.gov as NCT01295684. C1 [Novotny, Janet A.; Baer, David J.; Gebauer, Sarah K.; Charrons, Craig S.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Khoo, Christina] Ocean Spray Cranberries Inc, Lakeville Middleborough, MA USA. RP Novotny, JA (reprint author), ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Janet.Novotny@ars.usda.gov FU Ocean Spray Cranberries, Inc.; USDA FX Supported by Ocean Spray Cranberries, Inc., and the USDA. C Khoo and Ocean Spray Cranberries, Inc., provided the beverages used in this study and were involved in discussions about the design but were not involved in the conduct, analysis, or interpretation of the results NR 107 TC 15 Z9 15 U1 8 U2 30 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2015 VL 145 IS 6 BP 1185 EP 1193 DI 10.3945/jn.114.203190 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6ZI UT WOS:000355643300006 PM 25904733 ER PT J AU Marini, JC Agarwal, U Didelija, IC AF Marini, Juan C. Agarwal, Umang Didelija, Inka C. TI Dietary Arginine Requirements for Growth Are Dependent on the Rate of Citrulline Production in Mice SO JOURNAL OF NUTRITION LA English DT Article DE amino acid; arginine; citrulline; growth; requirements ID AMINO-ACID-REQUIREMENTS; ORNITHINE; METABOLISM; CHICKS; MOUSE AB Background: In many species, including humans, arginine is considered a semiessential amino acid because under certain conditions endogenous synthesis cannot meet its demand. The requirements of arginine for growth in mice are ill defined and seem to vary depending on the genetic background of the mice. Objective: The objective of this study was to determine the metabolic and molecular basis for the requirement of arginine in 2 mouse strains. Methods: Institute of Cancer Research (ICR) and C57BL/6 (BL6) male mice were fed arginine-free or arginine-sufficient diets (Expt. 1) or 1 of 7 diets with increasing arginine concentration (from 0- to 8-g/kg diet, Expt. 2) between day 24 and 42 of life to determine the arginine requirements for growth. Citrulline production and "de novo" arginine synthesis were measured with use of stable isotopes, and arginine requirements were determined by breakpoint analysis and enzyme expression by reverse transcriptase-polymerase chain reaction. Results: In Expt. 1, ICR mice grew at the same rate regardless of the arginine concentration of the diet (mean +/- SE: 0.66 +/- 0.04 g/d, P = 0.80), but BL6 mice had a reduced growth rate when fed the arginine-free diet (0.25 +/- 0.02 g/d, P < 0.001) compared to the 8-g arginine/kg diet (0.46 +/- 0.03 g/d). ICR mice showed at least a 2-fold greater expression (P < 0.001) of ornithine transcarbamylase (OTC) than BL6 mice, which translated into a greater rate of citrulline (25%) and arginine synthesis (49%, P < 0.002). In Expt. 2, breakpoint analysis showed that the requirement for growth of BL6 mice was met with 2.32 +/- 0.39 g arginine/kg diet; for ICR mice, however, no breakpoint was found. Conclusion: Our data indicate that a reduced expression of OTC in BL6 mice translates into a reduced production of citrulline and arginine compared with ICR mice, which results in a dietary arginine requirement for growth in BL6 mice, but not in ICR mice. C1 [Marini, Juan C.] Baylor Coll Med, Pediat Crit Care Med, Houston, TX 77030 USA. [Marini, Juan C.; Agarwal, Umang; Didelija, Inka C.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Marini, JC (reprint author), Baylor Coll Med, Pediat Crit Care Med, Houston, TX 77030 USA. EM marini@bcm.edu FU USDA, Agricultural Research Service [58-6250-6-001]; NIH [R01 GM108940] FX Supported by federal funds from the USDA, Agricultural Research Service, under Cooperative Agreement number 58-6250-6-001, and the NIH (R01 GM108940 to JCM). NR 25 TC 1 Z9 2 U1 2 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2015 VL 145 IS 6 BP 1227 EP 1231 DI 10.3945/jn.114.209668 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6ZI UT WOS:000355643300011 PM 25855119 ER PT J AU Sebastian, RS Enns, CW Goldman, JD Martin, CL Steinfeldt, LC Murayi, T Moshfegh, AJ AF Sebastian, Rhonda S. Enns, Cecilia Wilkinson Goldman, Joseph D. Martin, Carrie L. Steinfeldt, Lois C. Murayi, Theophile Moshfegh, Alanna J. TI A New Database Facilitates Characterization of Flavonoid Intake, Sources, and Positive Associations with Diet Quality among US Adults SO JOURNAL OF NUTRITION LA English DT Article DE USDA provisional flavonoid addendum; What We Eat in America; NHANES; flavonoid intake; flavonoid dietary sources; Healthy Eating Index ID CARDIOVASCULAR-DISEASE MORTALITY; MAJOR FOOD SOURCES; PROSPECTIVE COHORT; POSTMENOPAUSAL WOMEN; CANCER-RISK; HEALTH; POLYPHENOLS; METAANALYSIS; POPULATION; PREVENTION AB Background: Epidemiologic studies demonstrate inverse associations between flavonoid intake and chronic disease risk. However, lack of comprehensive databases of the flavonoid content of foods has hindered efforts to fully characterize population intakes and determine associations with diet quality. Objectives: Using a newly released database of flavonoid values, this study sought to describe intake and sources of total flavonoids and 6 flavonoid classes and identify associations between flavonoid intake and the Healthy Eating Index (HEI) 2010. Methods: One day of 24-h dietary recall data from adults aged >= 20 y (n = 5420) collected in What We Eat in America (WWEIA), NHANES 2007-2008, were analyzed. Flavonoid intakes were calculated using the USDA Flavonoid Values for Survey Foods and Beverages 2007-2008. Regression analyses were conducted to provide adjusted estimates of flavonoid intake, and linear trends in total and component HEI scores by flavonoid intake were assessed using orthogonal polynomial contrasts. All analyses were weighted to be nationally representative. Results: Mean intake of flavonoids was 251 mg/d, with flavan-3-ols accounting for 81% of intake. Non-Hispanic whites had significantly higher (P < 0.001) intakes of total flavonoids (275 mg/d) than non-Hispanic blacks (176 mg/d) and Hispanics (139 mg/d). Tea was the primary source (80%) of flavonoid intake. Regardless of whether the flavonoid contribution of tea was included, total HEI score and component scores for total fruit, whole fruit, total vegetables, greens and beans, seafood and plant proteins, refined grains, and empty calories increased (P < 0.001) across flavonoid intake quartiles. Conclusions: A new database that permits comprehensive estimation of flavonoid intakes in WWEIA, NHANES 2007 2008; identification of their major food/beverage sources; and determination of associations with dietary quality will lead to advances in research on relations between flavonoid intake and health. Findings suggest that diet quality, as measured by NEI, is positively associated with flavonoid intake. C1 [Sebastian, Rhonda S.; Enns, Cecilia Wilkinson; Goldman, Joseph D.; Martin, Carrie L.; Steinfeldt, Lois C.; Murayi, Theophile; Moshfegh, Alanna J.] ARS, Food Surveys Res Grp, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Sebastian, RS (reprint author), ARS, Food Surveys Res Grp, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Rhonda.Sebastian@ars.usda.gov FU Office of Dietary Supplements, NIH [Y1-OD-1365-01] FX Supported in part by the Office of Dietary Supplements, NIH, Agreement No. Y1-OD-1365-01. NR 82 TC 9 Z9 9 U1 2 U2 16 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2015 VL 145 IS 6 BP 1239 EP 1248 DI 10.3945/jn.115.213025 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6ZI UT WOS:000355643300013 PM 25948787 ER PT J AU Bandara, T Hettiarachchi, M Liyanage, C Amarasena, S Wong, WW AF Bandara, Thushari Hettiarachchi, Manjula Liyanage, Chandrani Amarasena, Sujeewa Wong, William W. TI The Deuterium Oxide-to-the-Mother Method Documents Adequate Breast-Milk Intake among Sri Lankan Infants SO JOURNAL OF NUTRITION LA English DT Article DE breast-milk production; deuterium oxide; infants; isotope ratio mass spectrometry; Sri Lanka ID VOLUME; WOMEN AB Background: The WHO recommends that exclusive breastfeeding should last up to 6 mo. However, human milk intake of Sri Lankan infants has not been quantified scientifically. Objectives: The objectives of this study were to measure the human milk intake of Sri Lankan infants during the first 6 mo of age and to document the breastfeeding practices of their mothers. Methods: Forty-eight healthy mother-infant dyads were randomly recruited for this cross-sectional study at well-baby clinics. Milk intake was measured using the deuterium oxide-to-the-mother technique over a period of 2 wk. Information on breastfeeding practice and living standards of the participants were gathered using an interviewer-administered questionnaire. Results: Human milk intake was 672 +/- 123 g . d(-1) (mean +/- SD), 776 +/- 212 g . d(-1), and 801 +/- 51 g . d(-1) for infants <2 mo, 2 to <4 mo, and 4-6 mo of age, respectively. The milk intakes were not different among the age groups. Maternal body composition, age, or parity had no effects on milk intake of the infants. However, mother's education (P < 0.05, r = 0.35), infant's age (P < 0.05, r = 0.30), and body mass index (P < 0.05, r = 0.41) positively correlated with the milk intake. Over 63% of mothers had commenced breastfeeding within 30 min of delivery. About 60% of the mothers were feeding the baby 6-10 times during the daytime and >81% intended to continue exclusive breastfeeding until 6 mo of age. Conclusion: This study, for the first time, documented the adequacy of breast-milk intake among Sri Lankan infants and the nutrition status of the mothers. C1 [Bandara, Thushari; Hettiarachchi, Manjula; Liyanage, Chandrani; Amarasena, Sujeewa] Univ Ruhuna, Fac Med, Matara, Sri Lanka. [Wong, William W.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Wong, WW (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. EM wwong@bcm.edu FU International Atomic Energy Agency (IAEA) [IAEA-SRI-16826] FX Supported by the International Atomic Energy Agency (IAEA), through research grant IAEA-SRI-16826. NR 26 TC 1 Z9 1 U1 1 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2015 VL 145 IS 6 BP 1325 EP 1329 DI 10.3945/jn.115.211771 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ6ZI UT WOS:000355643300024 PM 25904731 ER PT J AU Forsman, ED Sovern, SG Taylor, M Biswell, BL AF Forsman, Eric D. Sovern, Stan G. Taylor, Margaret Biswell, Brian L. TI HOME RANGE AND HABITAT SELECTION BY NORTHERN SPOTTED OWLS ON THE EASTERN SLOPE OF THE CASCADE MOUNTAINS, WASHINGTON SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Northern Spotted Owl; Strix occidentalis caurina; habitat selection; home range; fixed kernel; minimum convex polygon; radiotelemetry; Washington ID GENERALIZED ESTIMATING EQUATIONS; NEAREST-NEIGHBOR IMPUTATION; BARRED OWLS; RESOURCE SELECTION; MANAGED FORESTS; CALIFORNIA; OREGON; FUELS; SIZE; REPRODUCTION AB We used radiotelemetry to study space use and habitat selection of 16 Northern Spotted Owls (Strix occidentalis caurina) on the eastern slope of the Cascade Mountains, Washington, U.S.A., in 1989-1990. We used a geographical information system (GIS) and aerial photo interpretation of digital orthophotos to assign owl locations a value for vegetation type, topographic position, amount of edge, and distance to water. We compared owl relocations and random locations within 95% fixed kernel (FK) home ranges to determine each owl's selection of cover types, using logistic regression and generalized estimating equations (GEE) to estimate an exponential resource selection function likelihood. Minimum convex polygon (MCP) home ranges (SE) averaged 2858 ha (712 ha) for males and 1883 ha (249 ha) for females. Individual 95% FK home ranges averaged 1980 ha (229 ha) for males and 1649 ha (163 ha) for females. Pair home ranges averaged 3419 ha (826 ha) for MCP and 2427 ha (243 ha) for 95% FK. Nonbreeding season home ranges averaged approximately 3.5 times larger than breeding season home ranges for both males and females. Our best habitat model indicated that owls selected closed-canopy forests with a component of large (>= 50 cm dbh) trees for roosting and foraging. In a given cover type, owls foraged lower on the slope. Management circles centered on nest areas-commonly used as a surrogate for home ranges-can be relatively poor representations of actual ranges used by pairs. However, an alternative for managing Spotted Owl home ranges is not readily available. Maintaining sufficient closed-canopy forest to provide habitat for Spotted Owls in the dry, fire-prone forests on the eastern slope of the Washington Cascades will be a challenge because forestry methods used to reduce the risk or severity of fire generally reduce the prevalence of structural features that characterize good Spotted Owl habitat. C1 [Forsman, Eric D.] USDA, Forestry Sci Lab, Forest Serv, Corvallis, OR 97331 USA. [Sovern, Stan G.; Taylor, Margaret] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Biswell, Brian L.] USDA, Forestry Sci Lab, Forest Serv, Olympia, WA 98502 USA. RP Forsman, ED (reprint author), USDA, Forestry Sci Lab, Forest Serv, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM ssovern@fs.fed.us FU U.S.D.A. Forest Service, Pacific Northwest Research Station; Wenatchee National Forest FX We greatly appreciate the help of C. Foster, D. Rolph, and K. Maurice, who helped us collect the data. They endured many long days and nights of wind, rain, and snow, and usually seemed to love it. K. Dugger gave advice on the statistical analysis. K. Dugger, D. Wiens, and two anonymous reviewers provided useful comments that helped us to greatly improve on an earlier draft. P. Singleton provided the datasets for topographic position and solar insolation. This study was funded by the U.S.D.A. Forest Service, Pacific Northwest Research Station, and Wenatchee National Forest. NR 83 TC 0 Z9 0 U1 9 U2 51 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD JUN PY 2015 VL 49 IS 2 BP 109 EP 128 PG 20 WC Ornithology SC Zoology GA CJ2LQ UT WOS:000355316700001 ER PT J AU Singleton, PH AF Singleton, Peter H. TI FOREST STRUCTURE WITHIN BARRED OWL (STRIX VARIA) HOME RANGES IN THE EASTERN CASCADE RANGE, WASHINGTON SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Northern Spotted Owl; Strix occidentalis caurina; Barred Owl; Strix varia; forest management; habitat use; niche partitioning; telemetry ID SPOTTED OWLS; SITE CHARACTERISTICS; HABITAT SELECTION; SPACE USE; MOUNTAINS; DYNAMICS AB Competitive interactions with Barred Owls (Strix varia) are an important factor contributing to the decline of the Northern Spotted Owl (Strix occidentalis caurina) population. Understanding the degree of similarity in fine-scale habitat associations for Spotted Owls and Barred Owls will help land managers evaluate whether there are specific vegetation conditions that could favor Spotted Owls over Barred Owls. From March 2004 to September 2006, I tracked 14 radio-tagged Barred Owls in the Okanogan-Wenatchee National Forest in the eastern Cascade Range, Washington. I analyzed forest structure characteristics from 170 plots sampled within areas used by the radio-tagged owls. I identified three forest types present within the Barred Owl home ranges, including: (1) open ponderosa pine (Pinus ponderosa), (2) simple-structure Douglas-fir (Pseudotsuga menziesii), and (3) complex-structure grand fir (Abies grandis). I compared individual forest structure characteristics and the three forest types to the intensity of Barred Owl use based on repeated measures of seasonal utilization distribution values at each plot using hierarchical mixed-effects models. Intensity of Barred Owl use during the breeding season was higher in areas with greater abundance of grand fir trees, taller and more diverse tree heights, more total trees per ha, more trees 12.7-22.9 cm dbh, more tree canopy >4.9 m, and less ground-cover vegetation <0.6 m. During the nonbreeding season, intensity of Barred Owl use was higher in areas with more trees 12.7-22.9 cm dbh, more total trees per ha, gentle slopes, and increased tree species diversity. Barred Owls used the structurally diverse grand fir forest type more intensively than the other two types during the breeding season. Intensity of use did not differ across the types during the nonbreeding season. Forest structure characteristics used by Barred Owls in this study were within the range of conditions reported to be used by Spotted Owls in the eastern Cascade Range. C1 USDA, Pacific NW Res Stn, Forest Serv, Wenatchee, WA 98801 USA. RP Singleton, PH (reprint author), USDA, Pacific NW Res Stn, Forest Serv, 1133 North Western Ave, Wenatchee, WA 98801 USA. EM psingleton@fs.fed.us FU U.S. Fish and Wildlife Service; U.S.D.A. Forest Service, Pacific Northwest Research Station; Okanogan-Wenatchee National Forest FX Support for this project was provided by the U.S. Fish and Wildlife Service, U.S.D.A. Forest Service, Pacific Northwest Research Station, and Okanogan-Wenatchee National Forest. Scott Graham, Aja Woodrow, James Begley, Danielle Clay, Marlene Farrell, Elizabeth Goulet, MaryEllen Haggard, Scott Heller, John Meriwether, Gail Roberts, and Tom Walker contributed to the collection of the vegetation and radiotelemetry data used here. Josh Lawler, John Lehmkuhl, Martin Raphael, Ashley Steel, Joe Buchanan, Dave Wiens, and Stan Sovern provided reviews and discussions that contributed to the development and improvement of this manuscript. NR 26 TC 3 Z9 3 U1 3 U2 22 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD JUN PY 2015 VL 49 IS 2 BP 129 EP 140 PG 12 WC Ornithology SC Zoology GA CJ2LQ UT WOS:000355316700002 ER PT J AU Washburn, BE Begier, MJ Wright, SE AF Washburn, Brian E. Begier, Michael J. Wright, Sandra E. TI COLLISIONS BETWEEN EAGLES AND AIRCRAFT: AN INCREASING PROBLEM IN THE AIRPORT ENVIRONMENT SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Bald Eagle; Haliaeetus leucocephalus; Golden Eagle; Aquila chrysaetos; airport; bird strike; human-raptor conflicts ID GOLDEN EAGLES; BALD EAGLE; MANAGEMENT; POPULATION; MORTALITY; RANKING; RISK; US AB Most known fatalities for both Bald Eagles (Haliaeetus leucocephalus) and Golden Eagles (Aquila chrysaetos) are associated with humans (e.g., collisions with vehicles and artificial structures). Notably, the risk of collisions between eagles and aircraft is an increasing problem at civil airports and military airfields. Of the 234 eagle collisions with civil and military aircraft reported to the Federal Aviation Administration, the U.S. Air Force, and the U.S. Navy during 1990-2013, 52% caused damage to the aircraft. During this 23-yr time period, Bald Eagle-aircraft collisions increased by 2200% and Golden Eagle-aircraft collisions increased by 400%. Eagle-aircraft collisions occur primarily during daylight hours (88%) and typically within the vicinity of the airfield itself; 82.6% of the Bald Eagle-aircraft collisions and 81.0% of Golden Eagle strikes occurred when the aircraft was at or below 305 m aboveground level. Although collision with aircraft is a very minor source of mortality for Golden Eagles, increasing and expanding Bald Eagle populations will likely result in more eagle-aircraft collisions. Currently, there are few mitigation tools and techniques available to reduce eagle-aircraft collisions. Development and evaluation of effective, publically acceptable methods of reducing eagle-human conflicts represent important areas for future research. C1 [Washburn, Brian E.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Begier, Michael J.] Wildlife Serv, USDA, Washington, DC 20250 USA. [Wright, Sandra E.] USDA, Wildlife Serv, Sandusky, OH 44870 USA. RP Washburn, BE (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov FU Federal Aviation Administration; U.S. Air Force; U.S. Department of Agriculture FX The Federal Aviation Administration, the U.S. Air Force, and the U.S. Department of Agriculture provided valuable logistical support, advice, and funding. We thank D. Sullivan, Lt. A. Robertson, Lt. Commander T. Mackey, and P. Miller for assisting with data access and various aspects of the study. T. DeVault, T. Katzner, and D. Ellis provided helpful comments on earlier drafts of the manuscript. Opinions expressed in this study do not necessarily reflect current U.S. Department of Defense or Federal Aviation Administration policy decisions governing the control of wildlife on or near airports. NR 29 TC 0 Z9 0 U1 8 U2 22 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD JUN PY 2015 VL 49 IS 2 BP 192 EP 200 PG 9 WC Ornithology SC Zoology GA CJ2LQ UT WOS:000355316700008 ER PT J AU Dorr, BS Engle, CR AF Dorr, Brian S. Engle, Carole R. TI Influence of Simulated Double-crested Cormorant, Phalacrocorax auritus, Predation on Multiple-batch Production of Channel Catfish, Ictalurus punctatus SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID DELTA REGION; MISSISSIPPI; IMPACT; AQUACULTURE; DEPREDATION; INDUSTRY AB The double-crested cormorant, Phalacrocorax auritus, is considered the primary depredating bird species on commercially produced channel catfish, Ictalurus punctatus, in the southeastern USA. We simulated different levels of cormorant predation on losses at harvest and economic effects on channel catfish production in a multiple-batch cropping system. We observed significant (P<0.05) declines in catfish production at increasing levels of cormorant predation in this study. This decline was mitigated by increased individual growth of catfish at higher predation rates (i.e., lower catfish densities). This mitigating effect produced a non-linear relationship with total kg of catfish harvested per pond resulting in a non-linear incremental increase in breakeven price related to predation. Costs of production ($/kg) increased with increasing predation levels up to very high levels of predation with a cumulative maximum increase in breakeven price of $0.143/kg. These results indicate that losses at harvest due to cormorant predation occur immediately but are mitigated in part by compensatory growth of individual catfish. Losses due to cormorant predation in multi-batch systems can be considerable, but there is not a 1:1 relationship between losses and kg of catfish harvested due to compensatory factors. C1 [Dorr, Brian S.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Mississippi Field Stn, Mississippi State, MS 39762 USA. [Engle, Carole R.] Univ Arkansas Pine Bluff, Pine Bluff, AR 71601 USA. RP Dorr, BS (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, Mississippi Field Stn, POB 6099, Mississippi State, MS 39762 USA. EM brian.s.dorr@aphis.usda.gov FU USDA/WS/National Wildlife Research Center [QA-1047] FX USDA/WS/National Wildlife Research Center provided funding to conduct this research under Quality Assurance protocol QA-1047. We thank Ed Robinson and staff with the Mississippi State University Agriculture and Forestry Experiment Station and the USDA, WS, NWRC Mississippi Field Station for obtaining catfish and assistance with stocking, seining, and maintenance of catfish. We also thank K. C. Hanson-Dorr, S. C. Barras, B. Strickland, and an anonymous reviewer for their comments and editorial assistance. NR 22 TC 0 Z9 0 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD JUN PY 2015 VL 46 IS 3 BP 319 EP 327 DI 10.1111/jwas.12187 PG 9 WC Fisheries SC Fisheries GA CJ7MK UT WOS:000355679500009 ER PT J AU Mischke, CC Tucker, CS Wise, DJ Brown, TW AF Mischke, Charles C. Tucker, Craig S. Wise, David J. Brown, Travis W. TI DEET (N,N-diethyl-m-toluamide) Toxicity to Channel Catfish, Ictalurus punctatus, Sac Fry SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID INSECT REPELLENT DEET AB The combination of open facilities, moisture, and warm weather during channel catfish, Ictalurus punctatus, spawning season causes mosquito-infestation problems. A common solution to mosquito problems in hatcheries is to use mosquito repellents applied to exposed skin and clothing. DEET (N,N-diethyl-m-toluamide) is the active ingredient in most personal insect repellents. We determined the 24-h acute toxicity of DEET to channel catfish fry. The toxicity test consisted of 10 sac fry in three replications of six concentrations (range: 157-478L/L) and a control. In addition to toxicity testing, a trial was conducted to determine the amount of active ingredient dispensed from two different applicators: a pump sprayer and an aerosol can. The 24-h lethal concentration (LC10) was 274ppm, and the 24-h LC50 was 345ppm. The concentration required to kill 50% of the organisms was above the 100ppm threshold to be considered practically non-toxic. The pump sprayer (98.11% active ingredient) dispensed 113.3 +/- 0.57mg (mean +/- SEM) active ingredient per pump. The aerosol can (30% active ingredient) dispensed 526.8 +/- 6.71mg (mean +/- standard error mean [SEM]) active ingredient per second. In hatcheries where air movement by fans is not sufficient to control mosquitoes, using insect repellent products containing DEET should be safe. C1 [Mischke, Charles C.; Wise, David J.] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. [Tucker, Craig S.; Brown, Travis W.] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. RP Mischke, CC (reprint author), Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, 127 Expt Stn Rd, Stoneville, MS 38776 USA. EM cmischke@drec.msstate.edu FU MAFES [MIS-371590] FX The information provided in this article was obtained from the Mississippi Agricultural and Forestry Experiment Station (MAFES, 12562), Mississippi State University. This project is supported under MAFES Project Number MIS-371590. NR 13 TC 0 Z9 0 U1 5 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD JUN PY 2015 VL 46 IS 3 BP 344 EP 347 DI 10.1111/jwas.12191 PG 4 WC Fisheries SC Fisheries GA CJ7MK UT WOS:000355679500012 ER PT J AU Pfannenstiel, RS Ruder, MG AF Pfannenstiel, Robert S. Ruder, Mark G. TI Colonization of bison (Bison bison) wallows in a tallgrass prairie by Culicoides spp (Diptera: Ceratopogonidae) SO JOURNAL OF VECTOR ECOLOGY LA English DT Article ID VARIIPENNIS COMPLEX DIPTERA; WASTE-WATER PONDS; BLUETONGUE-VIRUS; UNITED-STATES; EPIDEMIOLOGY; DISEASE C1 [Pfannenstiel, Robert S.; Ruder, Mark G.] ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS 66502 USA. RP Ruder, MG (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS 66502 USA. EM mark.ruder@ars.usda.gov NR 19 TC 0 Z9 0 U1 4 U2 10 PU SOC VECTOR ECOLOGY PI CORONA PA 1966 COMPTON AVE, CORONA, CA 92881 USA SN 1081-1710 EI 1948-7134 J9 J VECTOR ECOL JI J. Vector Ecol. PD JUN PY 2015 VL 40 IS 1 BP 187 EP 190 DI 10.1111/jvec.12150 PG 4 WC Entomology SC Entomology GA CJ9VN UT WOS:000355851400024 PM 26047201 ER PT J AU Tarbill, GL Manley, PN White, AM AF Tarbill, G. L. Manley, P. N. White, A. M. TI Drill, baby, drill: the influence of woodpeckers on post-fire vertebrate communities through cavity excavation SO JOURNAL OF ZOOLOGY LA English DT Article DE burn severity; community ecology; ecological succession; community dynamics; pioneer species; post-fire habitat ID NEST-SITE SELECTION; PRESCRIBED FIRE; POPULATION-DYNAMICS; SIERRA-NEVADA; FORESTS; SUCCESS; BIRDS; MANAGEMENT; CANADA; SNAGS AB Several studies have addressed the importance of woodpeckers as ecological engineers in forests due to their excavation of cavities. Although research in green, unburned forests has identified the influence of different excavators on secondary use by cavity-dependent species, little is known about the relative importance of cavities created by woodpeckers in recently burned forests. By excavating cavities, woodpeckers create habitat for secondary cavity users that can facilitate post-fire regeneration through seed dispersal, seed germination and regulation of insect populations that affect vegetative growth. In this study, we monitored 77 cavities created by three species of Picoides woodpeckers for use by secondary cavity species in a fire that burned in the Sierra Nevada, California. At each cavity we measured nest tree and site-specific parameters to determine if these characteristics could explain differential use by secondary cavity users. We found substantial overlap in cavity characteristics between woodpecker species, with the white-headed woodpecker differing most notably in their placement of cavities in larger diameter, shorter and more decayed trees in less dense stands than either hairy or black-backed woodpeckers. These differences in cavity placement may have resulted in the high diversity and large number of detections of secondary cavity species in white-headed woodpecker cavities. Black-backed and hairy woodpeckers were similar in the number of detections of secondary cavity use, although black-backed woodpecker cavities were used by more species than hairy woodpecker cavities. Secondary cavity use was high (86%) suggesting these woodpeckers, and the white-headed woodpecker in particular, can have an accelerating affect effect on ecological succession by providing valuable habitat features for seed dispersing birds and mammals, insectivorous birds, and small predators, thereby impacting ecological processes and functions. C1 [Tarbill, G. L.; White, A. M.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95616 USA. [Tarbill, G. L.] Calif State Univ Sacramento, Dept Biol, Sacramento, CA 95819 USA. [Manley, P. N.] US Forest Serv, Pacific Southwest Res Stn, Inst Forest Genet, USDA, Placerville, CA USA. RP Tarbill, GL (reprint author), US Forest Serv, Pacific Southwest Res Stn, USDA, 1731 Res Pk Dr, Davis, CA 95616 USA. EM gtarbill@gmail.com FU USDA Forest Service, Pacific Southwest Research Station; Southern Nevada Public Lands Act; California Tahoe Conservancy; Department of Biology at Sacramento State University FX Financial support for this project was provided by the USDA Forest Service, Pacific Southwest Research Station, the Southern Nevada Public Lands Act, the California Tahoe Conservancy and the Department of Biology at Sacramento State University. T.W. Richardson assisted with methodology and data collection. J. Baldwin provided statistical advice. E. Newkirk assisted with database management. Field assistance was provided by D. Gaube, K. Knox, K. Zummo, B.R. Campos and S.E. Meloy. Forestry data were provided by C. Carlson and S.Z. Dobrowski of the University of Montana College of Forestry and Conservation and H.D. Safford of the USDA Forest Service, Pacific Southwest Region. Finally, we would like to thank and acknowledge the contribution that P. Drapeau and anonymous reviewers made to this paper. NR 43 TC 4 Z9 4 U1 4 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0952-8369 EI 1469-7998 J9 J ZOOL JI J. Zool. PD JUN PY 2015 VL 296 IS 2 BP 95 EP 103 DI 10.1111/jzo.12220 PG 9 WC Zoology SC Zoology GA CK1XX UT WOS:000356003900002 ER PT J AU Rehder, DS Gundberg, CM Booth, SL Borges, CR AF Rehder, Douglas S. Gundberg, Caren M. Booth, Sarah L. Borges, Chad R. TI Gamma-Carboxylation and Fragmentation of Osteocalcin in Human Serum Defined by Mass Spectrometry SO MOLECULAR & CELLULAR PROTEOMICS LA English DT Article ID VITAMIN-K SUPPLEMENTATION; DIETARY PHYLLOQUINONE; CARBOXYGLUTAMIC ACID; BONE HEALTH; UNDERCARBOXYLATED OSTEOCALCIN; INSULIN-RESISTANCE; ENERGY-METABOLISM; OLDER WOMEN; GLA PROTEIN; MEN AB Serum osteocalcin (Oc) concentration is a highly specific measure of bone turnover, but its circulating proteoform(s) have not been well defined. Based on immunological methods, the major forms are thought to be the intact polypeptide and a large N-terminal-mid molecule fragment for which there is no consensus on the precise sequence. Vitamin K-dependent gamma (gamma)-carboxylated variants of Oc are also found in circulation but there have been no methods that can define how many of the three potential gamma-carboxyglutamic acid (Gla) residues are gamma-carboxylated or provide their relative abundances. Recent reports that uncarboxylated and partially gamma-carboxylated Oc forms have hormonal function underscore the need for precise evaluation of Oc at all three potential gamma-carboxylation sites. Herein, mass spectrometric immunoassay (MSIA) was used to provide qualitative and semiquantitative (relative percent abundance) information on Oc molecular variants as they exist in individual plasma and serum samples. Following verification that observable Oc proteoforms were accurately assigned and not simply ex vivo artifacts, MALDI-MSIA and ESI-MSIA were used to assess the relative abundance of Oc truncation and gamma-carboxylation, respectively, in plasma from 130 patients enrolled in vitamin K supplementation trials. Human Oc was found to circulate in over a dozen truncated forms with each of these displaying anywhere from 0-3 Gla residues. The relative abundance of truncated forms was consistent and unaffected by vitamin K supplementation. In contrast, when compared with placebo, vitamin K supplementation dramatically increased the fractional abundance of Oc with three Gla residues, corresponding to a decrease in the fractional abundance of Oc with zero Gla residues. These findings unequivocally document that increased vi-tamin K intake reduces the uncarboxylated form of Oc. Several reports of a positive effect of vitamin K intake on insulin sensitivity in humans have shown that un-or undercarboxylation of Oc, unlike in mice, is not associated with insulin resistance. Analyses similar to those described here will be useful to understand the functional significance of Oc gamma-carboxylation in human health and disease. C1 [Rehder, Douglas S.; Borges, Chad R.] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA. [Borges, Chad R.] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA. [Gundberg, Caren M.] Yale Univ, Sch Med, Dept Orthopaed & Rehabil, New Haven, CT 06510 USA. [Booth, Sarah L.] Tufts Univ, Jean Mayer USDA Human Nutr Ctr Aging, Boston, MA 02111 USA. RP Borges, CR (reprint author), Arizona State Univ, Dept Chem & Biochem, POB 876401, Tempe, AZ 85287 USA. EM chad.borges@asu.edu FU National Institutes of Health [DK082542, DK090958, AR38460, AG14759, DK69341]; USDA, Agricultural Research Service [58-1950-7-707] FX This work was supported by: The National Institutes of Health DK082542 and DK090958 (DR, CB), AR38460 (CG), AG14759 (SB) and DK69341 (SB), and the USDA, Agricultural Research Service under Cooperative Agreement No. 58-1950-7-707). NR 43 TC 4 Z9 4 U1 1 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 1535-9476 EI 1535-9484 J9 MOL CELL PROTEOMICS JI Mol. Cell. Proteomics PD JUN PY 2015 VL 14 IS 6 BP 1546 EP 1555 DI 10.1074/mcp.M114.047621 PG 10 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CJ5SX UT WOS:000355550400009 PM 25855755 ER PT J AU Liu, HJ Wang, XQ Warburton, ML Wen, WW Jin, ML Deng, M Liu, J Tong, H Pan, QC Yang, XH Yan, JB AF Liu, Haijun Wang, Xiaqing Warburton, Marilyn L. Wen, Weiwei Jin, Minliang Deng, Min Liu, Jie Tong, Hao Pan, Qingchun Yang, Xiaohong Yan, Jianbing TI Genomic, Transcriptomic, and Phenomic Variation Reveals the Complex Adaptation of Modern Maize Breeding SO MOLECULAR PLANT LA English DT Article DE temperate adaptation; positive selection; regulatory evolution; Zea mays ID BOX PROTEIN TIR1; GENE-EXPRESSION; SEED DEVELOPMENT; FLOWERING-TIME; ARABIDOPSIS-THALIANA; PLANT DEVELOPMENT; WIDE ASSOCIATION; AUXIN RECEPTOR; ZEA-MAYS; STRESS AB The temperate-tropical division of earlymaize germplasms to different agricultural environments was arguably the greatest adaptation process associated with the success and near ubiquitous importance of global maize production. Deciphering this history is challenging, but new insight has been gained from examining 558 529 single nucleotide polymorphisms, expression data of 28 769 genes, and 662 traits collected from 368 diverse temperate and tropical maize inbred lines in this study. This is a new attempt to systematically exploit the mechanisms of the adaptation process in maize. Our results indicate that divergence between tropical and temperate lines apparently occurred 3400-6700 years ago. Seven hundred and one genomic selection signals and transcriptomic variants including 2700 differentially expressed individual genes and 389 rewired co-expression network genes were identified. These candidate signals were found to be functionally related to stress responses, and most were associated with directionally selected traits, which may have been an advantage under widely varying environmental conditions faced by maize as it was migrated away from its domestication center. Our study also clearly indicates that such stress adaptation could involve evolution of protein-coding sequences as well as transcriptome-level regulatory changes. The latter process may be a more flexible and dynamic way for maize to adapt to environmental changes along its short evolutionary history. C1 [Liu, Haijun; Wang, Xiaqing; Wen, Weiwei; Jin, Minliang; Deng, Min; Liu, Jie; Tong, Hao; Pan, Qingchun; Yan, Jianbing] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. [Warburton, Marilyn L.] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Yang, Xiaohong] China Agr Univ, Natl Maize Improvement Ctr China, Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China. RP Yan, JB (reprint author), Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. EM yjianbing@mail.hzau.edu.cn FU National Natural Science Foundation of China [31123009, 31222041]; National Basic Research and Development Program of China [2011CB100105]; National Hi-Tech Research and Development Program of China [2012AA10A307]; Fundamental Research Funds for the Central Universities [2013SC01] FX This research was supported by the National Natural Science Foundation of China (31123009, 31222041), the National Basic Research and Development Program of China (2011CB100105), the National Hi-Tech Research and Development Program of China (2012AA10A307), and the Fundamental Research Funds for the Central Universities (Program No. 2013SC01). NR 102 TC 9 Z9 9 U1 5 U2 28 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1674-2052 EI 1752-9867 J9 MOL PLANT JI Mol. Plant. PD JUN 1 PY 2015 VL 8 IS 6 BP 871 EP 884 DI 10.1016/j.molp.2015.01.016 PG 14 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CJ5UZ UT WOS:000355558600004 PM 25620769 ER PT J AU Zhou, Q Miao, H Li, S Zhang, SP Wang, Y Weng, YQ Zhang, ZH Huang, SW Gu, XF AF Zhou, Qian Miao, Han Li, Shuai Zhang, Shengping Wang, Ye Weng, Yiqun Zhang, Zhonghua Huang, Sanwen Gu, Xingfang TI A Sequencing-Based Linkage Map of Cucumber SO MOLECULAR PLANT LA English DT Letter ID CYTOGENETIC MAP; GENOME; DOMESTICATION C1 [Zhou, Qian; Miao, Han; Li, Shuai; Zhang, Shengping; Wang, Ye; Zhang, Zhonghua; Huang, Sanwen; Gu, Xingfang] Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Inst Vegetables & Flowers, Minist Agr,Sino Dutch Joint Lab Hort Gen, Beijing 10081, Peoples R China. [Zhou, Qian; Huang, Sanwen] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China. [Weng, Yiqun] Univ Wisconsin, Vegetable Crops Res Unit, Dept Hort, USDA,ARS, Madison, WI 53706 USA. RP Huang, SW (reprint author), Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Inst Vegetables & Flowers, Minist Agr,Sino Dutch Joint Lab Hort Gen, Beijing 10081, Peoples R China. EM zhangzhonghua@caas.cn; huangsanwen@caas.cn; guxingfang@caas.cn NR 10 TC 3 Z9 3 U1 2 U2 21 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1674-2052 EI 1752-9867 J9 MOL PLANT JI Mol. Plant. PD JUN 1 PY 2015 VL 8 IS 6 BP 961 EP 963 DI 10.1016/j.molp.2015.03.008 PG 3 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CJ5UZ UT WOS:000355558600012 PM 25792147 ER PT J AU Honda, KL Lamon-Fava, S Matthan, NR Wu, DY Lichtenstein, AH AF Honda, Kaori L. Lamon-Fava, Stefania Matthan, Nirupa R. Wu, Dayong Lichtenstein, Alice H. TI Docosahexaenoic acid differentially affects TNF alpha and IL-6 expression in LPS-stimulated RAW 264.7 murine macrophages SO PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS LA English DT Review DE TNF alpha; IL-6; TLR4; Macrophages; PGE(2); CREB ID NECROSIS-FACTOR-ALPHA; NF-KAPPA-B; POLYUNSATURATED FATTY-ACIDS; PROSTAGLANDIN E-2 RECEPTORS; ELEMENT-BINDING PROTEIN; TOLL-LIKE RECEPTORS; MEDIATED TRANSCRIPTION; EICOSAPENTAENOIC ACID; INFLAMMATORY RESPONSE; NUCLEAR-PROTEIN AB Docosahexaenoic acid (DHA) is generally reported to have anti-inflammatory properties, however, prior work has documented differential effects on individual pro-inflammatory cytokines: reduced IL-6, but not TNF alpha, mRNA expression in macrophages. To elucidate the mechanism, the roles of prostaglandin E-2 (PGE(2)), cyclic AMP response element-binding protein (CREB), and NF kappa B were examined in RAW 264.7 macrophages. DMA did not influence CREB activity, but significantly reduced PGE(2) production by 41% and NF kappa B activity by 32%. Exogenous PGE(2) inhibited TNF alpha mRNA expression dose dependently. Unexpectedly, inhibiting PGE(2) production with NS-398 also decreased TNF alpha mRNA expression, suggesting a concentration-dependent dual role of PGE(2) in regulating TNF alpha expression. IL-6 expression was unaffected by endogenous or exogenous PGE(2). Partial block of NF kappa B activation (SN50; 46%, or, BAY-11-7082; 41%) lowered IL-6 to a greater extent than TNF alpha mRNA expression. The differential effect of DMA on TNF alpha and IL-6 mRNA expression may be mediated via reduction in NF kappa B activity. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Honda, Kaori L.; Lamon-Fava, Stefania; Matthan, Nirupa R.; Wu, Dayong; Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM alice.lichtenstein@tufts.edu FU NIH [NHLBI-T32-HL069772]; USDA [58-1950-0-0014] FX This study was supported by grants from the NIH NHLBI-T32-HL069772 (NLS) and the USDA agreement No. 58-1950-0-0014. NR 63 TC 6 Z9 6 U1 2 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0952-3278 EI 1532-2823 J9 PROSTAG LEUKOTR ESS JI Prostaglandins Leukot. Essent. Fatty Acids PD JUN PY 2015 VL 97 BP 27 EP 34 DI 10.1016/j.plefa.2015.03.002 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism GA CJ7VH UT WOS:000355707900005 PM 25921297 ER PT J AU Kudva, IT Krastins, B Torres, AG Griffin, RW Sheng, HQ Sarracino, DA Hovde, CJ Calderwood, SB John, M AF Kudva, Indira T. Krastins, Bryan Torres, Alfredo G. Griffin, Robert W. Sheng, Haiqing Sarracino, David A. Hovde, Carolyn J. Calderwood, Stephen B. John, Manohar TI The Escherichia coli O157:H7 cattle immunoproteome includes outer membrane protein A (OmpA), a modulator of adherence to bovine rectoanal junction squamous epithelial (RSE) cells SO PROTEOMICS LA English DT Article DE Cattle; GeLC-MS; MS; Immunoproteome; Norepinephrine; O157 ID III SECRETED PROTEINS; O157-H7 ADHERENCE; VIRULENCE FACTORS; TERMINAL RECTUM; STRESS-RESPONSE; FEEDLOT CATTLE; SHIGA TOXIN; BEEF-CATTLE; INFECTION; COLONIZATION AB Building on previous studies, we defined the repertoire of proteins comprising the immunoproteome (IP) of Escherichia coli O157:H7 (O157) cultured in DMEM supplemented with norepinephrine (O157 IP), a -adrenergic hormone that regulates E. coli O157 gene expression in the gastrointestinal tract, using a variation of a novel proteomics-based platform proteome mining tool for antigen discovery, called proteomics-based expression library screening (PELS; Kudva etal., 2006). The E. coli O157 IP (O157-IP) comprised 91 proteins, and included those identified previously using proteomics-based expression library screening, and also proteins comprising DMEM and bovine rumen fluid proteomes. Outer membrane protein A (OmpA), a common component of the above proteomes, and reportedly a contributor to E. coli O157 adherence to cultured HEp-2 epithelial cells, was interestingly found to be a modulator rather than a contributor to E. coli O157 adherence to bovine rectoanal junction squamous epithelial cells. Our results point to a role for yet to be identified members of the O157-IP in E. coli O157 adherence to rectoanal junction squamous epithelial cells, and additionally implicate a possible role for the outer membrane protein A regulator, TdcA, in the expression of such adhesins. Our observations have implications for the development of efficacious vaccines for preventing E. coli O157 colonization of the bovine gastrointestinal tract. C1 [Kudva, Indira T.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Krastins, Bryan; Sarracino, David A.] Harvard Partners Ctr Genet & Genom, Cambridge, MA USA. [Torres, Alfredo G.] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA. [Torres, Alfredo G.] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA. [Griffin, Robert W.; Calderwood, Stephen B.; John, Manohar] Massachusetts Gen Hosp, Div Infect Dis, Boston, MA 02114 USA. [Sheng, Haiqing; Hovde, Carolyn J.] Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83843 USA. [Calderwood, Stephen B.; John, Manohar] Harvard Univ, Sch Med, Dept Med, Boston, MA USA. [Calderwood, Stephen B.; John, Manohar] Harvard Univ, Sch Med, Dept Microbiol & Immunobiol, Boston, MA USA. RP Kudva, IT (reprint author), ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM Indira.Kudva@ars.usda.gov FU US National Institutes of Health (NIH) [R21 AI055963]; National Research Initiative of the US Department of Agriculture Cooperative State Research, Education and Extension Service [99-35201-8539, 04-04562]; US NIH [NO1-HD-0-3309, U54-AI-57141, P20-RR16454, P20-RR15587, P20-GM103408] FX US National Institutes of Health (NIH) grants R21 AI055963 to I.T.K supported part of this work. Excellent technical assistance provided by Bryan Wheeler at the National Animal Disease Center (NADC), Ames, Iowa, with the eukaryotic cell adherence/adherence-inhibition assays is acknowledged. The Animal Resource Unit personnel at NADC, especially Dr. Rebecca Madison and Denise Chapman, are gratefully acknowledged for assisting with the collection of the bovine rectoanal junction tissues and swabs. C.J.H was supported in part by the National Research Initiative of the US Department of Agriculture Cooperative State Research, Education and Extension Service grants 99-35201-8539 and 04-04562 and US NIH grants NO1-HD-0-3309, U54-AI-57141, P20-RR16454, P20-RR15587, and P20-GM103408 NR 61 TC 1 Z9 1 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1615-9853 EI 1615-9861 J9 PROTEOMICS JI Proteomics PD JUN PY 2015 VL 15 IS 11 BP 1829 EP 1842 DI 10.1002/pmic.201400432 PG 14 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CJ8CR UT WOS:000355727400010 PM 25643951 ER PT J AU Tuttle, KM Martinez, SA Schramm, EC Takebayashi, Y Seo, M Steber, CM AF Tuttle, Keiko M. Martinez, Shantel A. Schramm, Elizabeth C. Takebayashi, Yumiko Seo, Mitsunori Steber, Camille M. TI Grain dormancy loss is associated with changes in ABA and GA sensitivity and hormone accumulation in bread wheat, Triticum aestivum (L.) SO SEED SCIENCE RESEARCH LA English DT Article DE abscisic acid; afterripening; cold stratification; dormancy; gibberellin; pre-harvest sprouting; Triticum aestivum ID WHITE WINTER-WHEAT; SEED DORMANCY; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; SPROUTING-RESISTANT; SPRING WHEAT; GIBBERELLIN BIOSYNTHESIS; INSENSITIVE MUTANTS; IMPOSED DORMANCY; LOW-TEMPERATURE AB Knowledge about the hormonal control of grain dormancy and dormancy loss is essential in wheat, because low grain dormancy at maturity is associated with the problem of pre-harvest sprouting (PHS) when cool and rainy conditions occur before harvest. Low GA (gibberellin A) hormone sensitivity and high ABA (abscisic acid) sensitivity were associated with higher wheat grain dormancy and PHS tolerance. Grains of two PHS-tolerant cultivars were very dormant at maturity, and insensitive to GA stimulation of germination. More PHS-susceptible cultivars were less sensitive to ABA inhibition of germination, and were either more GA sensitive or germinated efficiently without GA at maturity. As grain dormancy was lost through dry afterripening or cold imbibition, grains first gained GA sensitivity and then lost ABA sensitivity. These changes in GA and ABA sensitivity can serve as landmarks defining stages of dormancy loss that cannot be discerned without hormone treatment. These dormancy stages can be used to compare different cultivars, seed lots and studies. Previous work showed that wheat afterripening is associated with decreasing ABA levels in imbibing seeds. Wheat grain dormancy loss through cold imbibition also led to decreased endogenous ABA levels, suggesting that reduced ABA signalling is a general mechanism triggering dormancy loss. C1 [Tuttle, Keiko M.; Martinez, Shantel A.; Schramm, Elizabeth C.; Steber, Camille M.] Washington State Univ, Dept Crop & Soil Sci, Mol Plant Sci, Pullman, WA 99164 USA. [Takebayashi, Yumiko; Seo, Mitsunori] RIKEN, Ctr Sustainable Resource Sci, Yokohama, Kanagawa 2300045, Japan. [Steber, Camille M.] Washington State Univ, USDA ARS, Physiol & Dis Res Unit, Wheat Genet Qual, Pullman, WA 99164 USA. RP Steber, CM (reprint author), Washington State Univ, Dept Crop & Soil Sci, Mol Plant Sci, Pullman, WA 99164 USA. EM csteber@wsu.edu RI Seo, Mitsunori/N-9603-2015; Takebayashi, Yumiko/C-8190-2017 OI Takebayashi, Yumiko/0000-0003-4207-297X FU National Science Foundation's East Asia and Pacific Summer Institute Award [1209878]; Japanese Society for Promotion of Science Summer Internship Award; Washington Grain Commission; US Department of Agriculture-Agricultural Research Service FX This research was supported by the National Science Foundation's East Asia and Pacific Summer Institute Award (1209878 to K.M.T.), the Japanese Society for the Promotion of Science Summer Internship Award (to K.M.T. and M.S.), the Washington Grain Commission and the US Department of Agriculture-Agricultural Research Service (to C.M.S.). NR 73 TC 5 Z9 5 U1 4 U2 32 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0960-2585 EI 1475-2735 J9 SEED SCI RES JI Seed Sci. Res. PD JUN PY 2015 VL 25 IS 2 SI SI BP 179 EP 193 DI 10.1017/S0960258515000057 PG 15 WC Plant Sciences SC Plant Sciences GA CK0SE UT WOS:000355914400011 ER PT J AU Mun, S Sassenrath, GF Schmidt, AM Lee, N Wadsworth, MC Rice, B Corbitt, JQ Schneider, JM Tagert, ML Pote, J Prabhu, R AF Mun, S. Sassenrath, G. F. Schmidt, A. M. Lee, N. Wadsworth, M. C. Rice, B. Corbitt, J. Q. Schneider, J. M. Tagert, M. L. Pote, J. Prabhu, R. TI Uncertainty analysis of an irrigation scheduling model for water management in crop production SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Irrigation schedule modeling; Uncertainty analysis; Soil water balance; Agricultural production tools; Crop water management ID MAIZE; UNIFORMITY; EFFICIENCY AB Irrigation scheduling tools are critical to allow producers to effectively manage water resources for crop production. To be useful, these tools need to be accurate, complete, and relatively reliable. The current work presents an uncertainty analysis and its results for the Mississippi Irrigation Scheduling Tool (MIST) model, showing the margin of error (uncertainty) of the resulting irrigation advice arising solely from the propagation of measurement uncertainty through the MIST calculations. The final relative uncertainty in the water balance value from MIST was shown to be around 9% of that value, which is in the normal range of the margin of error and acceptable for agronomic systems. The results of this research also indicate that accurate measurements of irrigation and rainfall are critical to minimizing errors when using MIST and similar scheduling tools. While developed with data from Mississippi, the results of this uncertainty analysis are relevant to similar tool development efforts across the southern and southeastern United States and other high-rainfall areas, especially for locations lacking high-quality co-located weather stations. (C) 2015 Elsevier B.V. All rights reserved. C1 [Mun, S.; Lee, N.; Wadsworth, M. C.; Rice, B.; Tagert, M. L.; Pote, J.; Prabhu, R.] Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. [Sassenrath, G. F.] Kansas State Univ, Southeast Agr Res Ctr, Parsons, KS 67357 USA. [Schmidt, A. M.] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. [Corbitt, J. Q.] USDA ARS, Stoneville, MS 38776 USA. [Schneider, J. M.] WeatherSense LLC, Geary, OK 73040 USA. RP Prabhu, R (reprint author), Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. EM sm655@msstate.edu; gsassenrath@ksu.edu; aschmidt@unl.edu; nl145@msstate.edu; mcw229@msstate.edu; br264@msstate.edu; Jason.corbitt@ars.usda.gov; jeanneschneider@mac.com; mltagert@abe.msstate.edu; jpote@abe.msstate.edu; rprabhu@abe.msstate.edu OI Mun, Sungkwang/0000-0002-3347-2587 FU Mississippi Soybean Promotion Board; Mississippi Corn Promotion Board; Mississippi State University MAFES Special Research Initiative (SRI); Department of Agricultural and Biological Engineering at Mississippi State University FX Support for portions of this work was received from the Mississippi Soybean Promotion Board and the Mississippi Corn Promotion Board to A.M. Schmidt and G.F. Sassenrath and from Mississippi State University MAFES Special Research Initiative (SRI) 2013 to R. Prabhu and G. F. Sassenrath. The authors would like to thank the Department of Agricultural and Biological Engineering at Mississippi State University for supporting this work. Special thanks and appreciation go to the cooperating farmers for the on-farm studies of crop water use and model validation. This is contribution number 15-092-J from the Kansas Agricultural Experiment Station. NR 26 TC 2 Z9 2 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JUN PY 2015 VL 155 BP 100 EP 112 DI 10.1016/j.agwat.2015.03.009 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CI8PW UT WOS:000355035200010 ER PT J AU Grigg, ME Dubey, JP Nussenblatt, RB AF Grigg, Michael E. Dubey, Jitender P. Nussenblatt, Robert B. TI Ocular Toxoplasmosis: Lessons From Brazil SO AMERICAN JOURNAL OF OPHTHALMOLOGY LA English DT Editorial Material ID GLOBAL REASSESSMENT; MEMORIAL-LECTURE; SOUTHERN BRAZIL; GONDII; RETINOCHOROIDITIS; INFECTION; DISEASE; RECURRENCES; ANTIGENS; BLOOD C1 [Grigg, Michael E.] NIAID, Parasit Dis Lab, Mol Parasitol Sect, Div Intramural Res,NIH, Bethesda, MD 20892 USA. [Nussenblatt, Robert B.] NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Dubey, Jitender P.] USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Grigg, ME (reprint author), NIAID, Parasit Dis Lab, NIH, 4 Ctr Dr,Room B1-06, Bethesda, MD 20892 USA. EM griggm@niaid.nih.gov FU Intramural NIH HHS [ZIA AI001017-09] NR 29 TC 10 Z9 10 U1 1 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9394 EI 1879-1891 J9 AM J OPHTHALMOL JI Am. J. Ophthalmol. PD JUN PY 2015 VL 159 IS 6 BP 999 EP 1001 DI 10.1016/j.ajo.2015.04.005 PG 3 WC Ophthalmology SC Ophthalmology GA CI9DL UT WOS:000355070500001 PM 25956461 ER PT J AU Li, HK Wan, YZ Simon, CJ AF Li, H. K. Wan, Y. Z. Simon, C. J. TI Characterisation of linkage disequilibrium patterns among intra-gene single-nucleotide polymorphisms in Vitis based on resequencing of 30 gene fragments SO AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH LA English DT Article DE genetic association pattern; grape species; linkage disequilibrium; single-nucleotide polymorphism; (2) test ID DROSOPHILA-MELANOGASTER; VINIFERA L.; GENOME; ASSOCIATION; DIVERSITY; SELECTION; GRAPE; ARABIDOPSIS; RECOMBINATION; DOMESTICATION AB Background and AimsLinkage disequilibrium (LD) is an important tool in studies of genetics and evolution. In the cultivated species of Vitis vinifera, LD has been extensively surveyed; however, in other Vitis species, LD has not previously been reported. Patterns of LD in five populations of 48 Vitis species and 248 accessions were extensively characterised in this study. Methods and ResultsPatterns of LD were discerned using 6862 intra-gene single-nucleotide polymorphisms (SNPs) through resequencing 12.4kb from 30 gene fragments. Most SNPs (99.9-100.0%) in Vitis presented LD associations within fragments. Similar contribution between linkage and recombination to LD was found in four of the five populations from estimation of the entire dataset. More contribution, however, from recombination to LD was observed in most of the single fragments. With moderate frequency, LD may be more stable than that with either low or high frequency. Mantel tests showed that most correlation coefficients (Rxy) were non-significant between LD and physical distance (PD, bp); however, most significant Rxy had negative values, indicating that PD proximity may be an important contributor to LD. ConclusionsPatterns of LD differed extensively among the five populations, among gene fragments and among regions within fragments. Most SNPs were depicted near each other in the principal coordinates analysis plots, suggesting that these SNPs were associated with each other. Both advantages and disadvantages of LD association should be properly evaluated for genetic conservation and grape breeding. Significance of the StudyThis research not only greatly adds to our knowledge of LD association and other genetic features in the genus of Vitis, but also provides valuable suggestions for genetic conservation and grape breeding. C1 [Wan, Y. Z.] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. [Li, H. K.] Northwest A&F Univ, Coll Resources & Environm, Yangling 712100, Shaanxi, Peoples R China. [Simon, C. J.] Cornell Univ, USDA, ARS, Plant Genet Resources Unit,New York State Agr Exp, Geneva, NY 14456 USA. RP Wan, YZ (reprint author), Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. EM wyz689@hotmail.com FU National Yangling Bio-Tech Breeding Centre Project [2001-01]; China Scholarship Council Project [22861057]; Young Scientist Foundation of Northwest AF University [QN2009-013]; Fund for Returnees Scientists from Foreign Study in Shaanxi Province [A289021201]; USDA-ARS Plant Genetic Resources Unit Current Research Information System (CRIS) [1910-21000-020-00D] FX We are grateful to Dr Joanne A. Labate for making valuable comments and suggestions about this study, to Dr Angela M. Baldo for designing primers for resequencing and to Dr Peter Cousins (E. & J. Gallo Winery) for providing expert advice on grapevine identification and classification. United States Department of Agriculture is an equal opportunity provider and employer. This work was supported by the National Yangling Bio-Tech Breeding Centre Project (2001-01), China Scholarship Council Project (22861057), the Young Scientist Foundation of Northwest A&F University (QN2009-013), the 2012-Fund for Returnees Scientists from Foreign Study in Shaanxi Province (A289021201) and the USDA-ARS Plant Genetic Resources Unit Current Research Information System (CRIS) Project 1910-21000-020-00D. NR 43 TC 0 Z9 0 U1 2 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1322-7130 EI 1755-0238 J9 AUST J GRAPE WINE R JI Aust. J. Grape Wine Res. PD JUN PY 2015 VL 21 IS 2 BP 319 EP 330 DI 10.1111/ajgw.12128 PG 12 WC Food Science & Technology; Horticulture SC Food Science & Technology; Agriculture GA CJ1NT UT WOS:000355251100016 ER PT J AU Braham, M Miller, T Duerr, AE Lanzone, M Fesnock, A LaPre, L Driscoll, D Katzner, T AF Braham, Melissa Miller, Tricia Duerr, Adam E. Lanzone, Michael Fesnock, Amy LaPre, Larry Driscoll, Daniel Katzner, Todd TI Home in the heat: Dramatic seasonal variation in home range of desert golden eagles informs management for renewable energy development SO BIOLOGICAL CONSERVATION LA English DT Article DE Aquila cluysaetos; Desert Renewable Energy Conservation Plan; Golden eagle; Home range; Mojave desert; Renewable energy ID RESOURCE SELECTION; HABITAT USE; WIND; ESTIMATORS; PATTERNS; RAPTOR AB Renewable energy is expanding quickly with sometimes dramatic impacts to species and ecosystems. To understand the degree to which sensitive species may be impacted by renewable energy projects, it is informative to know how much space individuals use and how that space may overlap with planned development. We used global positioning system global system for mobile communications (GPS-GSM) telemetry to measure year-round movements of golden eagles (Aquila cluysaetos) from the Mojave Desert of California, USA. We estimated monthly space use with adaptive local convex hulls to identify the temporal and spatial scales at which eagles may encounter renewable energy projects in the Desert Renewable Energy Conservation Plan area. Mean size of home ranges was lowest and least variable from November through January and greatest in February March and May August. These monthly home range patterns coincided with seasonal variation in breeding ecology, habitat associations, and temperature. The expanded home ranges in hot summer months included movements to cooler, prey-dense, mountainous areas characterized by forest, grasslands, and scrublands. Breeding-season home ranges (October May) included more lowland semi-desert and rock vegetation. Overlap of eagle home ranges and focus areas for renewable energy development was greatest when eagle home ranges were smallest, during the breeding season. Golden eagles in the Mojave Desert used more space and a wider range of habitat types than expected and renewable energy projects could affect a larger section of the regional population than was previously thought. Published by Elsevier Ltd. C1 [Braham, Melissa] W Virginia Univ, Div Geol & Geog, Morgantown, WV 26506 USA. [Braham, Melissa; Miller, Tricia; Duerr, Adam E.; Katzner, Todd] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Lanzone, Michael] Cellular Tracking Technol, Somerset, PA 15501 USA. [Fesnock, Amy] Bur Land Management, Calif State Off, Sacramento, CA 95825 USA. [LaPre, Larry] Bur Land Management, Calif Desert Dist, Moreno Valley, CA 92553 USA. [Driscoll, Daniel] Amer Eagle Res Inst, Apache Junction, AZ 85117 USA. [Katzner, Todd] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID 83706 USA. [Katzner, Todd] US Forest Serv, USDA, Timber & Watershed Lab, Parsons, WV 26287 USA. RP Braham, M (reprint author), W Virginia Univ, Div Forestry & Nat Resources, POB 6125, Morgantown, WV 26506 USA. EM Melissa.Braham@mail.wvu.edu OI Katzner, Todd/0000-0003-4503-8435 FU Bureau of Land Management [L11PX02237] FX Funding for this work was provided by the Bureau of Land Management, grant agreement L11PX02237. This research was approved by the West Virginia University Animal Care and Use Committee (ACUC) protocol #11-0304. At the time the research was conducted, T.K. and M.L. were owners of Cellular Tracking Technologies that manufactured the GPS-GSM transmitters used in this project. AF, TK, LL, TM, AED and ML developed the project; DD and colleagues trapped the eagles; MB, TIC, AED and TM analyzed the data; MB and TIC led writing and all authors contributed to revising and improving the manuscript. Brian Millsap, Dave Wiens and three anonymous reviewers provided helpful comments to improve the manuscript. This is Scientific Article No. 3243 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 39 TC 8 Z9 8 U1 7 U2 47 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD JUN PY 2015 VL 186 BP 225 EP 232 DI 10.1016/j.biocon.2015.03.020 PG 8 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CI8ZX UT WOS:000355061300026 ER PT J AU Zarones, L Sussman, A Morton, JM Plentovich, S Faegre, S Aguon, C Amar, A Ha, RR AF Zarones, Lainie Sussman, Adrienne Morton, John M. Plentovich, Sheldon Faegre, Sarah Aguon, Celestino Amar, Arjun Ha, Renee Robinette TI Population status and nest success of the Critically Endangered Mariana Crow Corvus kubaryi on Rota, Northern Mariana Islands SO BIRD CONSERVATION INTERNATIONAL LA English DT Article ID BROWN TREE SNAKE; PACIFIC ISLAND; AVIFAUNA; TRENDS; PERSISTENCE; EXTINCTION; SELECTION; SURVIVAL; IMPACTS; BIRDS AB The Mariana Crow Corvus kubaryi is a Critically Endangered species found only on the island of Rota, Northern Mariana Islands. It was extirpated from the neighbouring island of Guam by the introduced brown tree snake Boiga irregularis and the Rota population has been in decline since at least 1995. We identified only 60 pairs present on Rota in 2007 compared with an estimated 117 pairs in 1998, a decline of nearly 50% in nine years. The decline may be linked to proximity to human activities, though more data are needed. We monitored 204 crow nests between the 1996 and 2009 breeding seasons. Crows initiate clutches between August and April. The overall estimate of nest success was 25.7% (n = 204). On average 49% of pairs produced at least one fledgling per season. The mean number of fledglings per pair per year is 0.66. Mean clutch size was 2.6 (n = 82), mean number of nestlings was 1.4 (n = 106), and mean number of fledglings per nest was 1.3 (n = 68). Daily survival rates declined in later years, and increased during the nest cycle. The number of pairs with successful nests did not change during the study period, nor did the number of fledglings per pair. Predation appeared to be the primary cause of nest failure. The breeding season lasted around nine months and pairs re-nested after failure. Predation of adults and juveniles by cats, combined with possible inbreeding depression, habitat disturbance and human persecution appear to be the cause of decline of the Mariana Crow. We strongly recommend a programme of invasive predator control, habitat maintenance, and captive rearing to ensure the species' survival. C1 [Zarones, Lainie; Sussman, Adrienne; Faegre, Sarah; Ha, Renee Robinette] Univ Washington, Dept Psychol, Seattle, WA 98195 USA. [Morton, John M.] US Fish & Wildlife Serv, Dededo, GU 96912 USA. [Plentovich, Sheldon] Univ Hawaii, Dept Zool, Honolulu, HI 96822 USA. [Aguon, Celestino] USDA, Div Aquat & Wildlife Resources, Mangilao, GU 96913 USA. [Amar, Arjun] CNMI, Div Fish & Wildlife, Rota, CM 96951 USA. RP Zarones, L (reprint author), Dept Lands & Nat Resources, POB 10007, Saipan, CM 96950 USA. EM lainiezarones@gmail.com RI Amar, Arjun /F-3815-2011 OI Amar, Arjun /0000-0002-7405-1180 FU Commonwealth of the Northern Mariana Islands; Division of Fish and Wildlife; U. S. Fish & Wildlife Service FX We are grateful for the critical reviews of earlier versions of this manuscript by anonymous reviewers. This study was only possible because of the excellent professionalism shown by numerous biologists over the years: F. Amidon, M. Awasthy, M. Colon, G. Desy, B. Dicke, R. Dymzarov, J. Esselstyn, S. Farry, J. Graham, P. Hannon, J. Herriges, S. Holman, B. Hughes, J. Jackson, N. Johnson, M. Knight, S. Lawson, T. Lee, K. Levenstein, K. Livengood, B. Massey, B. O'Neil, M. Parker, M. Philippart, S. Pruett, L. Pulliam, J. Quitugua, R. Reed, E. Rehm, T. Reid, G. Robinson, D. Rollinson, M. Rogne, S. Seibel, T. Sharp, J. Shousky, N. Shutt, J. Stanaway, L. Tanino, R. Ulloa, M. Vamstad, E. Weiser, E. Wells, C. Willet, S. Wyshynski, and additional staff over the years from Guam's Division of Aquatic and Wildlife Resources. L. Williams and S. Kremer provided logistical support, and S. Taisacan and D. Worthington provided field support, for work on Rota CNMI. We thank the many CNMI Department of Lands and Natural Resources Secretaries and Division of Fish and Wildlife Directors who have supported this work over the years, including A. Palacios, M. Pangelinan, I. Dela Cruz, S. Igisomar, R. Seman, and P. Hamilton. All research was conducted under Federal Endangered Species Permits FWSPIO-3, TE-032209-7/8 and TE-017352-14 Federal Bird Banding Permits 22570 and 22802, CNMI scientific collecting permit no. 01512, and an amended CNMI Fish and Game Permit 068-95-SPN. This work was financially supported by the Commonwealth of the Northern Mariana Islands, Division of Fish and Wildlife and the U. S. Fish & Wildlife Service (Endangered Species, Section 6). NR 36 TC 3 Z9 3 U1 4 U2 26 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0959-2709 EI 1474-0001 J9 BIRD CONSERV INT JI Bird Conserv. Int. PD JUN PY 2015 VL 25 IS 2 BP 220 EP 233 DI 10.1017/S0959270914000045 PG 14 WC Ornithology SC Zoology GA CJ2AY UT WOS:000355287800008 ER PT J AU Poland, TM Chen, YG Koch, J Pureswaran, D AF Poland, Therese M. Chen, Yigen Koch, Jennifer Pureswaran, Deepa TI Review of the emerald ash borer (Coleoptera: Buprestidae), life history, mating behaviours, host plant selection, and host resistance SO CANADIAN ENTOMOLOGIST LA English DT Article ID AGRILUS-PLANIPENNIS COLEOPTERA; SEX-PHEROMONE COMPONENT; NORTH-AMERICAN; FAIRMAIRE COLEOPTERA; FRAXINUS SPP.; SPECIES RESISTANT; NATURAL ENEMIES; MANCHURIAN ASH; BIOTIC FACTORS; URBAN FOREST AB As of summer 2014, the invasive emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), has become established in 24 states in the United States of America and has killed tens of millions of ash trees since its introduction into Michigan in the 1990s. Considerable research has been conducted on many aspects of EAB life history, natural history, ecology, and management strategies in an attempt to contain this devastating pest. In this article, we review the life history, mating behaviours, and host plant selection by EAB in North America as well as host resistance to EAB attack. C1 [Poland, Therese M.] US Forest Serv, USDA, No Res Stn, Lansing, MI 48910 USA. [Chen, Yigen] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA. [Koch, Jennifer] US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. [Pureswaran, Deepa] Canadian Forest Serv, Laurentian Forestry Ctr, Quebec City, PQ G1V 4C7, Canada. RP Poland, TM (reprint author), US Forest Serv, USDA, No Res Stn, 3101 Technol Blvd,Suite F, Lansing, MI 48910 USA. EM tpoland@fs.fed.us NR 82 TC 8 Z9 8 U1 17 U2 74 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2015 VL 147 IS 3 SI SI BP 252 EP 262 DI 10.4039/tce.2015.4 PG 11 WC Entomology SC Entomology GA CJ1WB UT WOS:000355274400002 ER PT J AU Bauer, LS Duan, JJ Gould, JR Van Driesche, R AF Bauer, Leah S. Duan, Jian J. Gould, Juli R. Van Driesche, Roy TI Progress in the classical biological control of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) in North America SO CANADIAN ENTOMOLOGIST LA English DT Article ID EMERALD ASH BORER; HYMENOPTERA-BRACONIDAE DORYCTINAE; RUSSIAN FAR-EAST; SPATHIUS-AGRILI; NATURAL ENEMIES; PARASITOIDS ATTACKING; SEASONAL ABUNDANCE; IMMATURE STAGES; CHESTNUT BORER; BIOTIC FACTORS AB First detected in North America in 2002, the emerald ash borer (EAB) (Agrilus planipennis Fairmaire; Coleoptera: Buprestidae), an invasive phloem-feeding beetle from Asia, has killed tens of millions of ash (Fraxinus Linnaeus; Oleaceae) trees. Although few parasitoids attack EAB in North America, three parasitoid species were found attacking EAB in China: the egg parasitoid Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) and two larval parasitoids Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) and Spathius agrili Yang (Hymenoptera: Braconidae). In 2007, classical biological control of EAB began in the United States of America after release of these three species was approved. In 2013, release of the larval parasitoids was approved in Canada. Research continues at study sites in Michigan, United States of America where the establishment, prevalence, and spread of O. agrili and T. planipennisi have been monitored since 2008. However, establishment of S. agrili remains unconfirmed in northern areas, and its release is now restricted to regions below the 40th parallel. In 2015, approval for release of Spathius galinae Belokobylskij (Hymenoptera: Braconidae), an EAB larval parasitoid from the Russian Far East, may be granted in the United States of America. Researchers are guardedly optimistic that a complex of introduced and native natural enemies will regulate EAB densities below a tolerance threshold for survival of ash species or genotypes in forested ecosystems. C1 [Bauer, Leah S.] US Forest Serv, USDA, No Res Stn, Lansing, MI 48910 USA. [Duan, Jian J.] ARS, USDA, Beneficial Insects Introduct Res Unit, Newark, DE 19713 USA. [Gould, Juli R.] Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. [Van Driesche, Roy] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. RP Bauer, LS (reprint author), US Forest Serv, USDA, No Res Stn, Lansing, MI 48910 USA. EM lbauer@fs.fed.us FU Department of Entomology at Michigan State University; United States Department of Agriculture, Forest Service; United States Department of Agriculture, Animal and Plant Health Inspection Service FX The authors thank our many coworkers, research associates, students, and colleagues who provided assistance, collaborations, and information on emerald ash borer, natural enemies, and biological control research in the United States of America, Canada, and China during the last 12 years. They greatly appreciate federal, state, county, city, township, and other landowners for granting us permission to use their lands for the long-term studies on EAB biological control and ash recovery. They also thank T. Perkins (Michigan State University) and T. Luther (United States Department of Agriculture, Forest Service) for preparing the maps, two anonymous reviewers for their helpful comments on an early draft of this manuscript, the Department of Entomology at Michigan State University for their ongoing support and laboratory space, and United States Department of Agriculture, Forest Service and United States Department of Agriculture, Animal and Plant Health Inspection Service for partial funding of this research. NR 90 TC 13 Z9 15 U1 6 U2 34 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2015 VL 147 IS 3 SI SI BP 300 EP 317 DI 10.4039/tce.2015.18 PG 18 WC Entomology SC Entomology GA CJ1WB UT WOS:000355274400006 ER PT J AU Smith, A Herms, DA Long, RP Gandhi, KJK AF Smith, Annemarie Herms, Daniel A. Long, Robert P. Gandhi, Kamal J. K. TI Community composition and structure had no effect on forest susceptibility to invasion by the emerald ash borer (Coleoptera: Buprestidae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID AGRILUS-PLANIPENNIS COLEOPTERA; NORTH-AMERICA; FRAXINUS SPP.; SOUTHEASTERN MICHIGAN; RESISTANCE; ECOLOGY; TREES; INFESTATIONS; ECOSYSTEMS; MANAGEMENT AB Emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a non-native, wood-boring beetle that has caused widespread mortality of ash (Fraxinus Linnaeus (Oleaceae)) in eastern North America. During 2004-2007, we determined whether forest community composition and structure of black (F. nigra Marshall), green (F. pennsylvanica Marshall), and white (F. americana Linnaeus) ash stands influenced their susceptibility to EAB invasion in southeast Michigan, United States of America. There was no relationship between EAB-induced ash decline or percentage mortality and any measure of community composition (tree species diversity, stand/ash density, total basal area, or relative dominance of ash). There was also no relationship between measures of EAB impact (density of EAB signs, ash decline rating, percentage ash mortality, or percentage infested ash) and forest attributes (ash/total stand density, basal area, ash importance, or stand diversity). Decline and mortality of black ash advanced more rapidly than that of white and green ash. Percentage mortality of ash increased from 51% to 93% during 2004-2007. Distance from the epicentre of the invasion was negatively correlated with ash mortality, but this relationship dissipated over time. Stand composition data suggests that ash will be replaced by Quercus Linnaeus (Fagaceae), Acer Linnaeus (Sapindaceae), and Tilia Linnaeus (Malvaceae); such vegetation changes will irreversibly alter the structure and function of these forests. C1 [Smith, Annemarie] US Green Bldg Council, Cent Ohio Chapter, Columbus, OH 43214 USA. [Smith, Annemarie; Herms, Daniel A.; Gandhi, Kamal J. K.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Long, Robert P.] US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. [Gandhi, Kamal J. K.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Gandhi, KJK (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. EM kjgandhi@uga.edu FU United States Department of Agriculture Forest Service Northeastern Research Station's programme; United States Department of Agriculture Forest Service Northern Research Station; D.B. Warnell School of Forestry and Natural Resources, University of Georgia; Ohio Agricultural Research and Development Center; Ohio State University FX This research was funded by the United States Department of Agriculture Forest Service Northeastern Research Station's programme "Research on Biological Invasions of Northeastern Forests", United States Department of Agriculture Forest Service Northern Research Station, the D.B. Warnell School of Forestry and Natural Resources, University of Georgia, and by state and federal funds appropriated to the Ohio Agricultural Research and Development Center and The Ohio State University. We appreciate the hard work of Diane Hartzler, Bryant Chambers, Dan Rice, Rodrigo Chorbadjian, and Alejandro Chiriboga (The Ohio State University), all of whom assisted with fieldwork. Brittany Barnes (University of Georgia) formatted the paper. NR 42 TC 5 Z9 5 U1 14 U2 51 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2015 VL 147 IS 3 SI SI BP 318 EP 328 DI 10.4039/tce.2015.8 PG 11 WC Entomology SC Entomology GA CJ1WB UT WOS:000355274400007 ER PT J AU Siegert, NW Mercader, RJ McCullough, DG AF Siegert, Nathan W. Mercader, Rodrigo J. McCullough, Deborah G. TI Spread and dispersal of emerald ash borer (Coleoptera: Buprestidae): estimating the spatial dynamics of a difficult-to-detect invasive forest pest SO CANADIAN ENTOMOLOGIST LA English DT Article ID AGRILUS-PLANIPENNIS COLEOPTERA; LONG-DISTANCE DISPERSAL; NORTH-AMERICA; URBAN FOREST; BIOLOGICAL INVASIONS; POPULATION-DENSITY; GRAVITY MODELS; OUTLIER SITES; MANAGEMENT; PATTERNS AB The emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a destructive invasive pest that threatens North American ash (Fraxinus (Oleaceae)) and inflicts substantial aesthetic, ecological, and economic damage in urban forests and rural woodlands. Understanding adult EAB dispersal and spread of infestations is critical to effectively manage and mitigate its potential impact in North America and elsewhere. Challenges of quantifying short-distance and long-distance movement of adult EAB in the field remain exceptionally difficult. Here we review our current understanding of EAB spread. Research to date suggests natural spread of EAB populations is variable and influenced by local environmental conditions, but limited to only a few km per year. In contrast, long-distance spread resulting from anthropogenic movement of infested ash material, such as nursery trees or firewood, can greatly increase local and regional rate of spread through the formation of satellite populations. Key areas in need of future research are highlighted. C1 [Siegert, Nathan W.] US Forest Serv, Northeastern Area State & Private Forestry, Forest Hlth Protect, Durham, NH 03824 USA. [Mercader, Rodrigo J.] Washburn Univ, Dept Biol, Topeka, KS 66621 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. RP Siegert, NW (reprint author), US Forest Serv, Northeastern Area State & Private Forestry, Forest Hlth Protect, Durham, NH 03824 USA. EM nwsiegert@fs.fed.us RI Mercader, Rodrigo/L-6550-2015 OI Mercader, Rodrigo/0000-0002-4904-2805 NR 57 TC 6 Z9 6 U1 6 U2 42 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2015 VL 147 IS 3 SI SI BP 338 EP 348 DI 10.4039/tce.2015.11 PG 11 WC Entomology SC Entomology GA CJ1WB UT WOS:000355274400009 ER PT J AU McCullough, DG Mercader, RJ Siegert, NW AF McCullough, Deborah G. Mercader, Rodrigo J. Siegert, Nathan W. TI Developing and integrating tactics to slow ash (Oleaceae) mortality caused by emerald ash borer (Coleoptera: Buprestidae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID AGRILUS-PLANIPENNIS COLEOPTERA; NORTH-AMERICA; FRAXINUS SPP.; URBAN FOREST; SYSTEMIC INSECTICIDE; EMAMECTIN BENZOATE; OUTLIER SITES; TREES; INVASION; POPULATIONS AB Practical and effective strategies to manage emerald ash borer (EAB) (Agrilus planipennis Fairmaire; Coleoptera: Buprestidae) are increasingly important given economic and ecological impacts of this invader. While EAB detection remains challenging, tactics are available to protect individual ash (Fraxinus Linnaeus; Oleaceae) trees and slow EAB population growth, thereby delaying ash mortality. Simulations with a coupled map lattice model, parameterised with data from numerous field studies, showed treating trees with a highly effective systemic insecticide (TREE-age; emamectin benzoate), would be more effective in slowing EAB population growth than girdling trees to attract ovipositing females then destroying trees before larvae develop, while harvesting ash to reduce available phloem would have the least effect. From 2008 to 2012, cooperators participated in a pilot project to implement an integrated strategy for an EAB infestation in Upper Michigan, United States of America. Ash was inventoried and EAB density monitored using girdled ash detection trees supplemented with baited artificial traps across the >750 km(2) project area. While only a tiny fraction of ash trees in the project area were girdled (444-855 trees annually) or injected with TREE-age (<360 roadside trees annually), and treatment distribution was restricted, both treatments slowed EAB population growth. Coupling TREE-age injections with other tactics will likely yield additive or synergistic outcomes. C1 [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Mercader, Rodrigo J.] Washburn Univ, Dept Biol, Topeka, KS 66621 USA. [Siegert, Nathan W.] US Forest Serv, State & Private Forestry, Northeastern Area, Durham, NH 03824 USA. RP McCullough, DG (reprint author), Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. EM mccullo6@msu.edu RI Mercader, Rodrigo/L-6550-2015 OI Mercader, Rodrigo/0000-0002-4904-2805 FU United States Department of Agriculture Forest Service Northeastern Area State and Private Forestry; American Recovery and Reinvestment Act FX The authors thank the other members of the SLAM Management Board, including John Bedford, Michigan Department of Agriculture; Robert Heyd, Michigan Department of Natural Resources; Amos Ziegler and Gabriel Carballo, Michigan State University; Andrew Storer and Brenda Owen, Michigan Technological University; and Steven Katovich, Therese Poland, and Noel Schneeberger, United States Department of Agriculture Forest Service, for their important contributions to this project. They gratefully acknowledge the many people who participated in other aspects of the SLAM project. They also thank Chris MacQuarrie, Krista Ryall, and Taylor Scarr for organising the 2013 EAB symposium. Funding for the SLAM pilot project including our evaluation work, were provided by the United States Department of Agriculture Forest Service Northeastern Area State and Private Forestry, and the American Recovery and Reinvestment Act. NR 62 TC 5 Z9 6 U1 2 U2 34 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2015 VL 147 IS 3 SI SI BP 349 EP 358 DI 10.4039/tce.2015.3 PG 10 WC Entomology SC Entomology GA CJ1WB UT WOS:000355274400010 ER PT J AU Penaluna, BE Railsback, SF Dunham, JB Johnson, S Bilby, RE Skaugset, AE AF Penaluna, Brooke E. Railsback, Steve F. Dunham, Jason B. Johnson, Sherri Bilby, Robert E. Skaugset, Arne E. TI The role of the geophysical template and environmental regimes in controlling stream-living trout populations SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID INDIVIDUAL-BASED MODELS; RIVER CONTINUUM CONCEPT; CUTTHROAT TROUT; THERMAL REQUIREMENTS; PACIFIC-NORTHWEST; LANDSCAPE ECOLOGY; HABITAT; SALMONIDS; FISH; ECOSYSTEMS AB The importance of multiple processes and instream factors to aquatic biota has been explored extensively, but questions remain about how local spatiotemporal variability of aquatic biota is tied to environmental regimes and the geophysical template of streams. We used an individual-based trout model to explore the relative role of the geophysical template versus environmental regimes on biomass of trout (Oncorhynchus clarkii clarkii). We parameterized the model with observed data from each of the four headwater streams (their local geophysical template and environmental regime) and then ran 12 simulations where we replaced environmental regimes (stream temperature, flow, turbidity) of a given stream with values from each neighboring stream while keeping the geophysical template fixed. We also performed single-parameter sensitivity analyses on the model results from each of the four streams. Although our modeled findings show that trout biomass is most responsive to changes in the geophysical template of streams, they also reveal that biomass is restricted by available habitat during seasonal low flow, which is a product of both the stream's geophysical template and flow regime. Our modeled results suggest that differences in the geophysical template among streams render trout more or less sensitive to environmental change, emphasizing the importance of local fish-habitat relationships in streams. C1 [Penaluna, Brooke E.; Johnson, Sherri] US Forest Serv, USDA, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. [Penaluna, Brooke E.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Railsback, Steve F.] Lang Railsback & Associates, Arcata, CA 95521 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis Res Grp, Corvallis, OR 97331 USA. [Bilby, Robert E.] Weyerhaeuser Co, Federal Way, WA 98063 USA. [Skaugset, Arne E.] Oregon State Univ, Dept Forest Engn, Corvallis, OR 97331 USA. RP Penaluna, BE (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM bepenaluna@fs.fed.us OI Railsback, Steven/0000-0002-5923-9847 FU Environmental Protection Agency STAR Grant; J Frances Allen scholarship from American Fisheries Society; US Geological Survey to Oregon State University; Watersheds Research Cooperative; Oregon State University FX BEP was funded by an Environmental Protection Agency STAR Grant, a J Frances Allen scholarship from American Fisheries Society, a grant from the US Geological Survey to Oregon State University, the Watersheds Research Cooperative, and various scholarships at Oregon State University. I. Arismendi, L. Ganio, J. Hall, M. Betts, and three anonymous reviewers provided helpful comments. A. Evans gave statistical advice, and K. Ronnenberg helped with graphical illustrations. The use of trade or firm names here are for reader information only and do not constitute endorsement of any product or service by the US Government. NR 65 TC 3 Z9 3 U1 2 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD JUN PY 2015 VL 72 IS 6 BP 893 EP 901 DI 10.1139/cjfas-2014-0377 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CJ1NW UT WOS:000355251400009 ER PT J AU Waters, WR Palmer, MV Stafne, MR Bass, KE Maggioli, MF Thacker, TC Linscott, R Lawrence, JC Nelson, JT Esfandiari, J Greenwald, R Lyashchenkof, KP AF Waters, W. Ray Palmer, Mitchell V. Stafne, Molly R. Bass, Kristin E. Maggioli, Mayara F. Thacker, Tyler C. Linscott, Rick Lawrence, John C. Nelson, Jeffrey T. Esfandiari, Javan Greenwald, Rena Lyashchenkof, Konstantin P. TI Effects of Serial Skin Testing with Purified Protein Derivative on the Level and Quality of Antibodies to Complex and Defined Antigens in Mycobacterium bovis-Infected Cattle SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; SEROLOGICAL DIAGNOSIS; GAMMA-INTERFERON; TUBERCULOSIS; AVIDITY; RESPONSES; DISEASE; ELISA; SURVEILLANCE; PATHOGENESIS AB Several serological tests designed to detect antibodies to immunodominant Mycobacterium bovis antigens have recently emerged as ancillary tests for the detection of bovine tuberculosis in cattle, particularly when used after the injection of purified protein derivative (PPD) for skin testing, which significantly boosts M. bovis-specific antibody responses. The present findings demonstrate the onset and duration of boosted antibody responses after the injection of M. bovis PPD for the caudal fold test (CFT) and Mycobacterium avium and M. bovis PPDs for the comparative cervical test (CCT), administered in series in cattle experimentally infected with M. bovis. While skin tests boosted the responses to certain antigens (i.e., MPB83 and MPB70), they did not affect the responses to other antigens (e.g., ESAT-6, CFP10, MPB59, and MPB64). Administration of the CCT 105 days after the CFT resulted in an even greater secondary boost in antibody responses to MPB83 and MPB70 and to a proteinase K-digested whole-cell sonicate (WCS-PK) of M. bovis. Both IgM and IgG contributed to the initial boost in the MPB83/MPB70-specific antibody response after the CFT. The secondary boost after the CCT was primarily due to increased IgG levels. Also, the avidity of antibodies to MPB83 and MPB70 increased after the CCT in M. bovis-infected cattle. The avidity of antibodies to the WCS-PK antigens increased in the interval between the CFT and the CCT but did not increase further after the CCT. Together, these findings demonstrate that the administration of PPDs for skin tests results in additive enhancement (i.e., when the CFT and CCT are performed in series), both qualitative and quantitative, of MPB83/MPB70-specific antibody responses. C1 [Waters, W. Ray; Palmer, Mitchell V.; Maggioli, Mayara F.; Thacker, Tyler C.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Stafne, Molly R.; Maggioli, Mayara F.] Iowa State Univ, Coll Vet Med, Ames, IA USA. [Bass, Kristin E.] Bethyl Labs, Montgomery, TX USA. [Linscott, Rick; Lawrence, John C.] IDEXX Labs, Westbrook, ME USA. [Nelson, Jeffrey T.] Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, USDA, Ames, IA USA. [Esfandiari, Javan; Greenwald, Rena; Lyashchenkof, Konstantin P.] Chembio Diagnost Syst Inc, Medford, NY USA. RP Waters, WR (reprint author), ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM ray.waters@ars.usda.gov NR 42 TC 3 Z9 3 U1 2 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 EI 1556-679X J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD JUN PY 2015 VL 22 IS 6 BP 641 EP 649 DI 10.1128/CVI.00119-15 PG 9 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA CJ0DL UT WOS:000355143500004 PM 25855555 ER PT J AU Corbalan-Tutau, MD Gomez-Abellan, P Madrid, JA Canteras, M Ordovas, JM Garaulet, M AF Corbalan-Tutau, M. D. Gomez-Abellan, P. Madrid, J. A. Canteras, M. Ordovas, J. M. Garaulet, M. TI Toward a chronobiological characterization of obesity and metabolic syndrome in clinical practice SO CLINICAL NUTRITION LA English DT Article DE Circadian; Obesity; Melatonin; Cortisol; Metabolic syndrome ID BODY-FAT DISTRIBUTION; SALIVARY CORTISOL; WRIST TEMPERATURE; CIRCADIAN SYSTEM; SKIN TEMPERATURE; SLEEP DURATION; MOTOR-ACTIVITY; NORMAL-WEIGHT; CLOCK GENES; RHYTHMS AB Background & aims: To test several circadian rhythm variables in a female population to identify the best tool to assess chronodisruption in obesity and metabolic syndrome (MetS) to define a score to be used for chronodisruption characterization in clinical practice. Methods: Anthropometric measurements and markers of circadian rhythms, such as sleep and feeding diary, Horne-Ostberg questionnaire, melatonin and cortisol measurements, and wrist temperature measurements, were determined. MetS variables were also analyzed. Study was conducted in 70 women. Data were subjected to factor analysis. Receiver operating characteristic curves were used as predictors of chronodisruption risk, and a score was calculated to classify the subjects of risk. Results: Factor analysis showed that the first-factor grouped variables were related to the skin temperature measurement. Second factor consisted of variables related to salivary cortisol levels and obesity-related measurements. Third factor included variables related to sleep-wake cycle. Fourth factor referred to peripheral temperature variables and included the classification of subjects according to the Horne-Ostberg questionnaire. To obtain a final punctuation we performed the weighted mean of the first four factors. The final range was from 27 to 57, mean value of 42. Punctuation was defined as the "chronodisruption score." Women displaying higher chronodisruption scores had higher MetS risk. Conclusion: The study demonstrates that wrist temperature recordings, together with two questions of sleep onset and offset, and one morning salivary cortisol determination could be enough to characterize the chronobiology of obesity and MetS, a new chronodisruption score was developed. (C) 2014 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. C1 [Corbalan-Tutau, M. D.; Gomez-Abellan, P.; Madrid, J. A.; Garaulet, M.] Univ Murcia, Fac Biol, Dept Physiol, E-30100 Murcia, Spain. [Canteras, M.] Univ Murcia, Dept Biostat, E-30100 Murcia, Spain. [Ordovas, J. M.] Tufts Univ, Sch Med, Human Nutr Res Ctr Aging, US Dept Agr, Boston, MA 02111 USA. [Ordovas, J. M.] CNIC, Dept Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, J. M.] IMDEA Alimentac, Madrid, Spain. RP Garaulet, M (reprint author), Univ Murcia, Fac Biol, Dept Physiol, Campus Espinardo S-N, E-30100 Murcia, Spain. EM garaulet@um.es FU Spanish Government of Science and Innovation [BFU2011-24720]; Seneca Foundation [15123/PI/10] FX This work was supported by The Spanish Government of Science and Innovation (Project No. BFU2011-24720) and the Seneca Foundation (Project No. 15123/PI/10). NR 39 TC 7 Z9 7 U1 2 U2 16 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0261-5614 EI 1532-1983 J9 CLIN NUTR JI Clin. Nutr. PD JUN PY 2015 VL 34 IS 3 BP 477 EP 483 DI 10.1016/j.clnu.2014.05.007 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CI8PT UT WOS:000355034900020 PM 24953771 ER PT J AU Accinelli, C Abbas, HK Vicari, A Shier, WT AF Accinelli, Cesare Abbas, Hamed K. Vicari, Alberto Shier, W. Thomas TI Evaluation of recycled bioplastic pellets and a sprayable formulation for application of an Aspergillus flavus biocontrol strain SO CROP PROTECTION LA English DT Article DE Biodegradable plastic; Aspergillus flavus; Aflatoxins; Recycled bioplastic; Biocontrol formulations; Maize (Corn, Zea mays L.) ID AFLATOXIN CONTAMINATION; BIOLOGICAL-CONTROL; CORN; MAIZE; BIOSYNTHESIS; VARIABILITY; STRATEGIES; REDUCTION; GENETICS; ECOLOGY AB Biocontrol of Aspergillus flavus using inoculated bioplastic granules has been proven to be effective under laboratory and field conditions. In the present study, the use of low-density pellets from recycled bioplastic as a biocontrol strain carrier was evaluated. Applying recycled bioplastic pellets inoculated with biocontrol A. flavus NRRL 30797 to two soils with different levels of indigenous A. flavus, resulted in a rapid 55% and 63% decrease in percent aflatoxigenicity in A. flavus isolates from low and high population soils, respectively. The decrease was significantly more rapid using lower-density recycled bioplastic pellets than using higher-density bioplastic granules, but aflatoxin production by indigenous soil A. flavus decreased to comparable levels in two months. A sprayable bioplastic formulation prepared from inoculated recycled bioplastic pellets was effective in delivering adherent biocontrol A. flavus to corn leaves, and in promoting a 4-fold increase in recovered non-aflatoxigenic A. flavus DNA one week later. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Accinelli, Cesare; Vicari, Alberto] Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. [Abbas, Hamed K.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Shier, W. Thomas] Univ Minnesota, Dept Med Chem, Coll Pharm, Minneapolis, MN 55455 USA. RP Accinelli, C (reprint author), Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. EM cesare.accinelli@unibo.it OI Accinelli, Cesare/0000-0001-8200-0108 NR 32 TC 2 Z9 2 U1 2 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2015 VL 72 BP 9 EP 15 DI 10.1016/j.cropro.2015.02.020 PG 7 WC Agronomy SC Agriculture GA CI9AU UT WOS:000355063600002 ER PT J AU Hall, DG George, J Lapointe, SL AF Hall, David G. George, Justin Lapointe, Stephen L. TI Further investigations on colonization of Poncirus trifoliata by the Asian citrus psyllid SO CROP PROTECTION LA English DT Article DE Huanglongbing; Citrus greening; Candidatus Liberibacter; Diaphorina citri ID DIAPHORINA-CITRI; HEMIPTERA PSYLLIDAE; KUWAYAMA HOMOPTERA; GREENING DISEASE; HOST-PLANT; BIOLOGY; TEMPERATURE; PARAMETERS; FLORIDA AB The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae) vectors a bacterium, 'Candidatus Liberibacter asiaticus' (CLas), associated with one of the world's most serious diseases of citrus, huanglongbing (HLB) (also known as citrus greening disease). There is no known cure for this disease, which severely reduces tree productivity and fruit quality and promotes tree decline and death. Most growers apply insecticides to control ACP as a tactic to prevent or reduce the incidence of HLB. Plant resistance to ACP may be a viable tactic for managing ACP and CLas. Resistance to colonization by ACP populations has been reported in the genotype Poncirus trifoliata L., which is cross compatible with citrus. The Citrus Research Center (CRC) in Riverside, California, maintains a large number of accessions of P. trifoliata. We conducted four free-choice experiments with 29 CRC accessions of P. trifoliata and found that ACP deposited fewer eggs on 19 of those accessions compared with susceptible sweet orange, Citrus sinensis (L.) Osbeck. Subsequent infestation densities of nymphs generally reflected reductions in oviposition but there were notable exceptions. Some accessions may be susceptible to oviposition but contain traits that confer antibiosis to nymphs. In no-choice experiments with seedling plants representing eight P. trifoliata accessions, oviposition rates were reduced compared with sweet orange and it appeared that factors associated with mature leaves were involved. In no-choice experiments with adult ACP confined to flush cuttings, large reductions were observed in the number of eggs and nymphs on each of five P. trifoliata accessions compared with sweet orange. Based on these results in conjunction with published reports, accessions were identified that consistently showed resistance to oviposition. However, the level of resistance in these accessions has been variable possibly due to environmental conditions, plant age, proximity of plants to susceptible germplasm, and other factors. Published by Elsevier Ltd. C1 [Hall, David G.; George, Justin; Lapointe, Stephen L.] ARS, USDA, Ft Pierce, FL 34945 USA. RP Hall, DG (reprint author), ARS, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM David.Hall@ars.usda.gov; Justin.George@ars.usda.gov; Stephen.Lapointe@ars.usda.gov FU Citrus Research & Development Foundation [853] FX We acknowledge and thank Matt Hentz, Kathy Moulton and Patrick Legler for assistance with this research project. This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. The research was partially funded by a grant (#853) from the Citrus Research & Development Foundation. NR 21 TC 2 Z9 2 U1 7 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2015 VL 72 BP 112 EP 118 DI 10.1016/j.cropro.2015.03.010 PG 7 WC Agronomy SC Agriculture GA CI9AU UT WOS:000355063600016 ER PT J AU Wang, JC Correll, JC Jia, Y AF Wang, J. C. Correll, J. C. Jia, Y. TI Characterization of rice blast resistance genes in rice germplasm with monogenic lines and pathogenicity assays SO CROP PROTECTION LA English DT Article DE Monogenic line; Rice blast; Resistance genes Pita/Pita2 and Pi9 ID NEAR-ISOGENIC LINES; ORYZA-SATIVA L.; PI-TA; MAGNAPORTHE-ORYZAE; DIFFERENTIAL SYSTEM; PYRICULARIA-GRISEA; UNITED-STATES; DNA MARKERS; REP-PCR; POPULATION AB Resistance (R) genes have been effectively deployed in preventing rice crop losses due to the fungus Magnaporthe oryzae. In the present study, we studied the interaction between 24 monogenetic lines, carrying at least one major R gene, Pia, Pib, Pii, Pik, Pik-h, Pik-m, Pik-p, Pik-s, Pish, Pit, Pita, Pita-2, Piz, Piz-t, Pi1, Piz-5, Pi3, Pi5(t), Pi7(t), Pi9, Pi11(t), Pi12(t), Pi19, or Pi20, with 14 commonly found races (isolates) of M. oryzae. We demonstrated that the monogenic lines carrying Pi9 and Pita-2 provided broad spectrum resistance and their resistance frequencies were 92.9% and 78.6%, respectively. The races (isolates), IB33(FLN9), IE1(ZN13), IB1(ZN15), IC1(120), IE1K(TM2), IA1(ARB20112-114), IB49(unnamed), IH1(unnamed), ID1(ZN42), and IC17(ZN57) of M. oryzae were highly virulent to monogenic lines, and more than 50% of the isolates were pathogenic across all monogenic lines, respectively. Genetic diversity of 16 races (isolates), two as controls, of M. oryzae was verified by repetitive element palindrome polymerase chain reaction (Rep-PCR). Based on these data, a useful strategy of pyramiding blast R genes against pathogenic M. oryzae races was proposed. Eight rice germplasms were identified to carry more than two blast R genes with pathogenicity assays and R gene specific primers. These findings are important for breeding for improved blast resistance in the USA and worldwide. Published by Elsevier Ltd. C1 [Wang, J. C.] JAAS, Inst Plant Protect, Changchun 130033, Peoples R China. [Correll, J. C.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Jia, Y.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Jia, Y (reprint author), ARS, USDA, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130E, Stuttgart, AR 72160 USA. EM Yulin.Jia@ars.usda.gov FU Jilin Province government [20100578, 20120754, 201402016NY]; State Administration of Foreign Experts Affairs, PRC [CG20112200017]; University of Arkansas Rice Research and Promotion Board FX We thank the Sciences & Technology Development Fund of Jilin Province government (20100578, 20120754, 201402016NY), the Training Project of the State Administration of Foreign Experts Affairs, PRC (CG20112200017), and the University of Arkansas Rice Research and Promotion Board in supporting this research. We thank IRRI for generously providing rice monogenetic lines and rice variety LTH. We also thank Lijuan Li of JAAS, Michael Lin, Ellen McWhirter, and Tracy Bianco of USDA-Agricultural Research Service DB NRRC for their excellent technical assistance. The U. S. Department of Agriculture is an equal opportunity provider and employer. NR 42 TC 1 Z9 2 U1 3 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2015 VL 72 BP 132 EP 138 DI 10.1016/j.cropro.2015.03.014 PG 7 WC Agronomy SC Agriculture GA CI9AU UT WOS:000355063600019 ER PT J AU Werner, SJ DeLiberto, ST Mangan, AM Pettit, SE Ellis, JW Carlson, JC AF Werner, Scott J. DeLiberto, Shelagh T. Mangan, Anna M. Pettit, Susan E. Ellis, Jeremy W. Carlson, James C. TI Anthraquinone-based repellent for horned larks, great-tailed grackles, American crows and the protection of California's specialty crops SO CROP PROTECTION LA English DT Article DE Corvus brachyrhynchos; Eremophila alpestris; Human-wildlife conflicts; Quiscalus mexicanus; Vertebrate pest; Wildlife damage management ID DAMAGE; STRATEGIES AB Specialty crops include fresh and dried fruits, vegetables, tree nuts, and horticultural and nursery crops. California accounts for 28% of the specialty crop acreage in the United States of America, including 72% of U.S. lettuce production (Lactuca sativa L.), 27% of U.S. melon production and 100% of U.S. almond production (Prunus dulcis L). We conducted controlled feeding experiments to evaluate an anthraquinone-based repellent for horned larks (Eremophila alpestris L.), great-tailed grackles (Quiscalus mexicanus Gmelin) and American crows (Corvus brachyrhynchos Brehm) associated with the depredation of California's lettuce, melon and almond crops, respectively. We observed 38-100% feeding repellency among horned larks offered wheat seeds (Triticum spp. L) treated with 168-3010 ppm anthraquinone during the concentration-response experiment. Great-tailed grackles exposed to rice seeds (Oryza sativa L) treated with 2060-35,400 ppm anthraquinone exhibited 90-100% repellency. We observed 80-100% repellency among American crows offered almonds treated with 2980-31,500 ppm anthraquinone. We predicted a threshold concentration of 5200 ppm anthraquinone for American crows offered treated almonds. Our laboratory efficacy data provide a reliable basis for planning future field applications of anthraquinone-based bird repellents for the protection of specialty crops. Supplemental field efficacy studies are necessary for the registration of avian repellents and the management of agricultural depredation caused by wild birds. Published by Elsevier Ltd. C1 [Werner, Scott J.; DeLiberto, Shelagh T.; Mangan, Anna M.; Pettit, Susan E.; Ellis, Jeremy W.; Carlson, James C.] Wildlife Serv, USDA, US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Werner, SJ (reprint author), Wildlife Serv, USDA, US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Scott.J.Wemer@aphis.usda.gov OI Ellis, Jeremy/0000-0003-4512-0906 FU California Department of Food and Agriculture [SCB10034] FX This research was supported in part by California Department of Food and Agriculture's Specialty Crop Block Grant to S.J.W. (Grant Agreement SCB10034). Our feeding experiments were conducted with Avipel (R) Shield repellent (Arkion (R) Life Sciences, New Castle, DE, USA). Corporate collaborations do not imply endorsement by the United States Department of Agriculture. We appreciate the National Wildlife Research Center animal care staff that provided daily care throughout our feeding experiments. We also thank D.A. Reid for constructive feedback from his review of our manuscript. NR 30 TC 5 Z9 5 U1 2 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2015 VL 72 BP 158 EP 162 DI 10.1016/j.cropro.2015.03.020 PG 5 WC Agronomy SC Agriculture GA CI9AU UT WOS:000355063600023 ER PT J AU Mengistu, A Kelly, HM Arelli, PR Bellaloui, N AF Mengistu, Alemu Kelly, Heather M. Arelli, Prakash R. Bellaloui, Nacer TI Effects of tillage, cultivar and fungicide on Phomopsis longicolla and Cercospora kukuchii in soybean SO CROP PROTECTION LA English DT Article DE Headline fungicide; Soybean seed borne diseases ID SEED DECAY; INFECTION; QUALITY; YIELD; GERMINATION; KIKUCHII; IRRIGATION; SEVERITY AB It has become a standard practice for farmers to use a no-till production system where crop residues are left on the soil surface rather than incorporated into the soil. This practice helps reduce soil erosion, conserve energy, increase soil moisture, and reduce erosion. However, many plant pathogens survive in the previous year's crop residue, increasing disease risk in reduced-tillage systems. The effect of tillage on seed borne diseases has not been objectively evaluated in side by side comparisons of till and no-till culture. We evaluated the impact of tillage and foliar fungicide on percent seed infection by Phomopsis Seed Decay (PSD) and Purple Seed Stain (PSS) over 4 years at the Milan Research and Education Center, Milan, TN. Analysis of variance indicated there was no significant difference (P <= 0.05) in PSD and PSS infection due to tillage. However, there was a significant interaction between fungicide and tillage where fungicide significantly reduced PSD in tilled but not in no-till plots. On the contrary, PSS increased in both tilled and no-till plots with fungicide application. These results suggest that fungicide programs under both till and no-till systems may alter the level of severity of these two seed borne diseases and may require further research to minimize the risk. Published by Elsevier Ltd. C1 [Mengistu, Alemu; Arelli, Prakash R.] USDA ARS, Crop Genet Res Unit, Jackson, TN 38301 USA. [Kelly, Heather M.] Univ Tennessee, Entomol & Plant Pathol, Jackson, TN 38301 USA. [Bellaloui, Nacer] Crop Genet Res Unit, Stoneville, MS 38776 USA. RP Mengistu, A (reprint author), USDA ARS, Crop Genet Res Unit, 605 Airways Blvd, Jackson, TN 38301 USA. EM Alemu.mengistu@ars.usda.gov FU USDA, Agricultural Research Service [6401-21220-002-00D]; Tennessee Soybean Promotion Board [11-02-P 20111 (R111017178)] FX The authors thank Debbie Boykin for help with data analysis and J. Deffenbaugh and J. Jordan for technical assistance. This research was funded by the USDA, Agricultural Research Service, Project number 6401-21220-002-00D and the Tennessee Soybean Promotion Board 11-02-P Mengistu 20111 (R111017178). NR 44 TC 0 Z9 0 U1 2 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2015 VL 72 BP 175 EP 181 DI 10.1016/j.cropro.2015.03.022 PG 7 WC Agronomy SC Agriculture GA CI9AU UT WOS:000355063600026 ER PT J AU Rinella, MJ Hammond, DH Bryant, AEM Kozar, BJ AF Rinella, Matthew J. Hammond, Darcy H. Bryant, Ana-Elisa M. Kozar, Brian J. TI High precipitation and seeded species competition reduce seeded shrub establishment during dryland restoration SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bayesian adaptive management; competition; dryland; grassland; restoration; seeding; shrub ID WYOMING BIG SAGEBRUSH; OLD-FIELD RESTORATION; GREATER SAGE-GROUSE; ADAPTIVE-MANAGEMENT; PLANT-COMMUNITIES; PERENNIAL GRASSES; SEEDLING ESTABLISHMENT; POPULATION-DYNAMICS; HERBACEOUS BIOMASS; SEMIARID SAVANNA AB Drylands comprise 40% of Earth's land mass and are critical to food security, carbon sequestration, and threatened and endangered wildlife. Exotic weed invasions, overgrazing, energy extraction, and other factors have degraded many drylands, and this has placed an increased emphasis on dryland restoration. The increased restoration focus has generated a wealth of experience, innovations and empirical data, yet the goal of restoring diverse, native, dryland plant assemblages composed of grasses, forbs, and shrubs has generally proven beyond reach. Of particular concern are shrubs, which often fail to establish or establish at trivially low densities. We used data from two Great Plains, USA coal mines to explore factors regulating shrub establishment. Our predictor data related to weather and restoration (e.g., seed rates, rock cover) variables, and our response data described shrub abundances on fields of the mines. We found that seeded non-shrubs, especially grasses, formed an important competitive barrier to shrub establishment: With every one standard deviation increase in non-shrub seed rate, the probability shrubs were present decreased similar to 0.1 and shrub cover decreased similar to 35%. Since new fields were seeded almost every year for >20 years, the data also provided a unique opportunity to explore effects of stochastic drivers (i.e., precipitation, year effects). With every one standard deviation increase in precipitation the first growing season following seeding, the probability shrubs were present decreased similar to 0.07 and shrub cover decreased similar to 47%. High precipitation appeared to harm shrubs by increasing grass growth/competition. Also, weak evidence suggested shrub establishment was better in rockier fields where grass abundance/competition was lower. Multiple lines of evidence suggest reducing grass seed rates below levels typically used in Great Plains restoration would benefit shrubs without substantially impacting grass stand development over the long term. We used Bayesian statistics to estimate effects of seed rates and other restoration predictors probabilistically to allow knowledge of the predictors' effects to be refined through time in an adaptive management framework. We believe this framework could improve restoration planning in a variety of systems where restoration outcomes remain highly uncertain and ongoing restoration efforts are continually providing new data of value for reducing the uncertainty. C1 [Rinella, Matthew J.] ARS, Ft Keogh Livestock & Range Res Lab, USDA, Miles City, MT 59301 USA. [Hammond, Darcy H.] Mississippi State Univ, Dept Forestry, Coll Forest Resources, Mississippi State, MS 39759 USA. [Bryant, Ana-Elisa M.] Virginia Polytech Inst & State Univ, Ctr Leadership Global Sustainabil, Blacksburg, VA 24061 USA. [Kozar, Brian J.] Terra Soil & Environm Solut, Bozeman, MT 59714 USA. RP Rinella, MJ (reprint author), ARS, Ft Keogh Livestock & Range Res Lab, USDA, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM Matt.rinella@ars.usda.gov NR 88 TC 3 Z9 3 U1 4 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2015 VL 25 IS 4 BP 1044 EP 1053 DI 10.1890/14-1110.1 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ0TT UT WOS:000355191900013 PM 26465041 ER PT J AU Adler, PR Mitchell, JG Pourhashem, G Spatari, S Del Grosso, SJ Parton, WJ AF Adler, Paul R. Mitchell, James G. Pourhashem, Ghasideh Spatari, Sabrina Del Grosso, Stephen J. Parton, William J. TI Integrating biorefinery and farm biogeochemical cycles offsets fossil energy and mitigates soil carbon losses SO ECOLOGICAL APPLICATIONS LA English DT Article DE barley straw; bioenergy; cellulosic ethanol; corn stover; crop residue; life cycle assessment; nitrous oxide; soil carbon; wheat straw ID DAILY SOLAR-RADIATION; CORN STOVER REMOVAL; ORGANIC-CARBON; RESIDUE MANAGEMENT; COMPLEX TERRAIN; TILLAGE; CROP; MATTER; PRECIPITATION; TEMPERATURE AB Crop residues are potentially significant sources of feedstock for biofuel production in the United States. However, there are concerns with maintaining the environmental functions of these residues while also serving as a feedstock for biofuel production. Maintaining soil organic carbon (SOC) along with its functional benefits is considered a greater constraint than maintaining soil erosion losses to an acceptable level. We used the biogeochemical model DayCent to evaluate the effect of residue removal, corn stover, and wheat and barley straw in three diverse locations in the USA. We evaluated residue removal with and without N replacement, along with application of a high-lignin fermentation byproduct (HLFB), the residue by-product comprised of lignin and small quantities of nutrients from cellulosic ethanol production. SOC always decreased with residue harvest, but the decrease was greater in colder climates when expressed on a life cycle basis. The effect of residue harvest on soil N2O emissions varied with N addition and climate. With N addition, N2O emissions always increased, but the increase was greater in colder climates. Without N addition, N2O emissions increased in Iowa, but decreased in Maryland and North Carolina with crop residue harvest. Although SOC was lower with residue harvest when HLFB was used for power production instead of being applied to land, the avoidance of fossil fuel emissions to the atmosphere by utilizing the cellulose and hemicellulose fractions of crop residue to produce ethanol (offsets) reduced the overall greenhouse gas (GHG) emissions because most of this residue carbon would normally be lost during microbial respiration. Losses of SOC and reduced N mineralization could both be mitigated with the application of HLFB to the land. Therefore, by returning the high-lignin fraction of crop residue to the land after production of ethanol at the biorefinery, soil carbon levels could be maintained along with the functional benefit of increased mineralized N, and more GHG emissions could be offset compared to leaving the crop residues on the land. C1 [Adler, Paul R.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Mitchell, James G.; Pourhashem, Ghasideh; Spatari, Sabrina] Drexel Univ, Dept Civil Architectural & Environm Engn, Philadelphia, PA 19104 USA. [Del Grosso, Stephen J.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. [Parton, William J.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Adler, PR (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM paul.adler@ars.usda.gov FU Department of Education under GAANN-RETAIN [P200A100117] FX James G. Mitchell was supported by the Department of Education under GAANN-RETAIN, Award Number P200A100117. The authors thank Drexel students Christine Speers and Aswathi John, who was supported in part by Drexel University's Office of the Provost, for assistance in revising DayCent model simulations. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 54 TC 1 Z9 1 U1 10 U2 26 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2015 VL 25 IS 4 BP 1142 EP 1156 DI 10.1890/13-1694.1 PG 15 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ0TT UT WOS:000355191900020 PM 26465048 ER PT J AU Pojednic, RM Ceglia, L Lichtenstein, AH Dawson-Hughes, B Fielding, RA AF Pojednic, Rachele M. Ceglia, Lisa Lichtenstein, Alice H. Dawson-Hughes, Bess Fielding, Roger A. TI Vitamin D receptor protein is associated with interleukin-6 in human skeletal muscle SO ENDOCRINE LA English DT Article DE Vitamin D; Vitamin D receptor; Skeletal muscle; Inflammation ID KAPPA-B ACTIVITY; OLDER-ADULTS; STRENGTH; EXERCISE; SUPPLEMENTATION; INFLAMMATION; RESISTANCE; CELLS; RATS; MASS AB Vitamin D is associated with skeletal muscle physiology and function and may play a role in intramuscular inflammation, possibly via the vitamin D receptor (VDR). We conducted two studies to examine (1) whether serum 25-hydroxyvitamin D (25OHD) and/or intramuscular VDR protein concentrations are associated with intramuscular interleukin-6 (IL-6) and/or tumor necrosis factor-alpha (TNF alpha); and (2) whether 16-week supplementation with vitamin D-3 alters intramuscular IL-6 and/or TNF alpha. Potential-related signaling pathways were also examined. Muscle biopsies of 30 older, mobility-limited adults were obtained at baseline. A subset of 12 women were supplemented with either 4,000 IU/day of vitamin D-3 (N = 5) or placebo (N = 7), and biopsies were repeated at 16 weeks. Serum 25OHD was measured, and intramuscular VDR, IL-6, and TNF alpha gene expressions and protein concentrations were analyzed. Baseline serum 25OHD was not associated with intramuscular IL-6 or TNF alpha gene expression or protein concentration. Baseline intramuscular VDR protein concentration, adjusted for baseline serum 25OHD, was positively associated with intramuscular IL-6 gene expression (n = 28; p = 0.04), but negatively associated with intramuscular IL-6 protein (n = 18; p = 0.03). Neither intramuscular IL-6 nor TNF alpha gene expression was different between placebo (n = 7) or vitamin D-3 supplementation groups (n = 5) after 16 weeks (p = 0.57, p = 0.11, respectively). These data suggest that VDR is a better predictor than serum 25OHD concentration of intramuscular IL-6 gene and protein expressions. A similar relationship was not observed for TNF alpha expression. Further, supplementation with 4,000 IU vitamin D-3 per day does not appear to affect intramuscular IL-6 or TNF alpha gene expression after 16 weeks. C1 [Pojednic, Rachele M.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ceglia, Lisa] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA USA. [Ceglia, Lisa; Dawson-Hughes, Bess] Tufts Univ, Bone Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Pojednic, RM (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM rachele.pojednic@joslin.harvard.edu FU USDA Agricultural Research Service [58-1950-7-707, 58-1950-0-014]; Dairy Research Institute; Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679]; Boston Nutrition/Obesity Research Center [DK046200]; NHLBI pre-doctoral training Grant [T32HL69772] FX This study is supported by the USDA Agricultural Research Service, under agreements No. 58-1950-7-707 (to BDH and LC) and No. 58-1950-0-014 (to RAF); The Dairy Research Institute (R.A.F.), Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679 to R.A.F.); the Boston Nutrition/Obesity Research Center (DK046200 to R.A.F.); and an NHLBI pre-doctoral training Grant (T32HL69772 to RMP). [Any opinions, findings, conclusion, or recommendation expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Agriculture.] NR 35 TC 3 Z9 3 U1 0 U2 4 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 1355-008X EI 1559-0100 J9 ENDOCRINE JI Endocrine PD JUN PY 2015 VL 49 IS 2 BP 512 EP 520 DI 10.1007/s12020-014-0505-6 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CJ1GD UT WOS:000355230500025 PM 25510525 ER PT J AU Luvizotto, RAM Nascimento, AF Miranda, NCM Wang, XD Ferreira, ALA AF Luvizotto, R. A. M. Nascimento, A. F. Miranda, N. C. M. Wang, X-D Ferreira, A. L. A. TI Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats SO HUMAN & EXPERIMENTAL TOXICOLOGY LA English DT Article DE Adiponectin; FoxO1; lycopene; obesity; SIRT1 ID INSULIN-RESISTANCE; SYSTEMIC INFLAMMATION; TRANSCRIPTION FACTOR; METABOLIC SYNDROME; GENE-EXPRESSION; SINGLET OXYGEN; PPAR-GAMMA; CAROTENOIDS; LEPTIN; MACROPHAGES AB Aim: To investigate whether lycopene can modulate adiponectin levels and SIRT1 and FoxO1 gene expression in the adipose tissue of diet-induced obese rats. Methods: Male Wistar rats were first fed with hypercaloric diet (HD, n = 12) for 6 weeks, and afterward, these rats were randomly assigned to receive HD (n = 6) or HD with lycopene-rich tomato oleoresin (equivalent to lycopene 10 mg/kg body weight (BW)/day, HD + L, n = 6) by gavage for additional 6 weeks. Plasma lycopene and adiponectin levels were analyzed by high-performance liquid chromatography and immunoassay, respectively. The messenger RNA (mRNA) expressions of adiponectin, Sirtuin 1 (SIRT1), Forkhead box O 1 (FoxO1), fatty acid translocase/cluster of differentiation 36 (FAT/CD36), and PPAR in adipose tissues were determined by quantitative polymerase chain reaction. Results: Lycopene was detected in the plasma of rats in HD + L group but not in the HD group. Although both BW and adiposity were not different between the two groups, there was a significant increase in both plasma concentration and mRNA expression of adiponectin in the adipose tissue of the HD + L group. In addition, the lycopene supplementation upregulated mRNA expressions of SIRT1, FoxO1, and FAT/CD36 but downregulated PPAR in adipose tissue of obese rats. Conclusion: These data suggest that lycopene, in the concentration used, is not toxic and also its health benefits in adipose tissue may play a role against obesity-related complications. C1 [Luvizotto, R. A. M.; Nascimento, A. F.] Fed Univ Mato Grosso UFMT, Inst Hlth Sci, BR-78557267 Sinop, MG, Brazil. [Luvizotto, R. A. M.; Miranda, N. C. M.; Ferreira, A. L. A.] Botucatu Med Sch UNESP, Dept Internal Med, Sao Paulo, Brazil. [Wang, X-D] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Luvizotto, RAM (reprint author), Fed Univ Mato Grosso UFMT, Alexandre Ferronato Ave,1200 Inst Hlth Sci, BR-78557267 Sinop, MG, Brazil. EM reluvizotto@yahoo.com RI Nascimento, Andre/K-8857-2015 FU Sao Paulo Research Foundation (FAPESP) [10/06100-9, 10/19746-4, 11/19934-8, 11/22786-0]; U.S. Department of Agriculture [1950-51000-056] FX We thank Sao Paulo Research Foundation for financial support (FAPESP #10/06100-9, #10/19746-4, #11/19934-8, #11/22786-0) and U.S. Department of Agriculture, under agreement No. 1950-51000-056. NR 54 TC 7 Z9 7 U1 2 U2 10 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0960-3271 EI 1477-0903 J9 HUM EXP TOXICOL JI Hum. Exp. Toxicol. PD JUN PY 2015 VL 34 IS 6 BP 612 EP 619 DI 10.1177/0960327114551395 PG 8 WC Toxicology SC Toxicology GA CJ2SI UT WOS:000355334400005 PM 25632967 ER PT J AU Budischak, SA Sakamoto, K Megow, LC Cummings, KR Urban, JF Ezenwa, VO AF Budischak, Sarah A. Sakamoto, Kaori Megow, Lindsey C. Cummings, Kelly R. Urban, Joseph F., Jr. Ezenwa, Vanessa O. TI Resource limitation alters the consequences of co-infection for both hosts and parasites SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE Parasite interaction; Immune trade-off; Disease ecology; Trophic framework; Tolerance; Resistance; Super shedder ID HELIGMOSOMOIDES-BAKERI INFECTION; NIPPOSTRONGYLUS-BRASILIENSIS; MYCOBACTERIUM-TUBERCULOSIS; DIETARY-PROTEIN; DEFICIENT MICE; WOOD MICE; NUTRITION; RESISTANCE; IMMUNITY; IMMUNOLOGY AB Most animals are concurrently infected with multiple parasite species and live in environments with fluctuating resource availability. Resource limitation can influence host immune responses and the degree of competition between co-infecting parasites, yet its effects on individual health and pathogen transmission have not been studied for co-infected hosts. To test how resource limitation affects immune trade-offs and co-infection outcomes, we conducted a factorial experiment using laboratory mice. Mice were given a standard or low protein diet, dosed with two species of helminths (alone and in combination), and then challenged with a microparasite. Using a community ecology trophic framework, we found that co-infection influenced parasite survival and reproduction via host immunity, but the magnitude and direction of responses depended on resources and the combination of co-infecting parasites. Our findings highlight that resources and their consequence for host defenses are a key context that shapes the magnitude and direction of parasite interactions. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. C1 [Budischak, Sarah A.; Ezenwa, Vanessa O.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Sakamoto, Kaori; Cummings, Kelly R.; Ezenwa, Vanessa O.] Univ Georgia, Coll Vet Med, Athens, GA 30602 USA. [Megow, Lindsey C.] Univ Georgia, Coll Publ Hlth, Athens, GA 30602 USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. RP Budischak, SA (reprint author), 140 E Green St, Athens, GA 30602 USA. EM sabudischak@gmail.com OI Urban, Joseph/0000-0002-1590-8869 FU United States National Science Foundation (NSF) [DEB-1110459]; U.S. National Center for Research Resources [9T350D010433-06]; U.S. Department of Agriculture/Agricultural Research Service (USDA/ARS) [8042-32000-094-00D]; U.S. NSF Ecology of Infectious Diseases Grant [DEB-1102493] FX We thank L. Sellers, J. Alagappan, A. Zehner, O. Kamau-Nataki, B. Bringuel, N. Patel, S. Helms, K. Fontleroy, A. Harding, R. Trench, L Mclean, A. Maryniak, E. Rich, K. Yi and M. Martin for assistance in the laboratory. R. Lankau, S. Altizer, J. Byers, J. Maron, members of the Ezenwa laboratory and two anonymous reviewers provided helpful comments on earlier drafts. Animal protocols were approved by the University of Georgia, USA, Institutional Animal Care and Use Committee. This work was supported by a United States National Science Foundation (NSF), Doctoral Dissertation Improvement Grant to SAB and VOE (DEB-1110459), a U.S. National Center for Research Resources, training fellowship to KC (9T350D010433-06), a U.S. Department of Agriculture/Agricultural Research Service (USDA/ARS) Project Plan to JFU (8042-32000-094-00D), and a U.S. NSF Ecology of Infectious Diseases Grant, to VOE (DEB-1102493). NR 39 TC 7 Z9 7 U1 3 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 EI 1879-0135 J9 INT J PARASITOL JI Int. J. Parasit. PD JUN PY 2015 VL 45 IS 7 BP 455 EP 463 DI 10.1016/j.ijpara.2015.02.005 PG 9 WC Parasitology SC Parasitology GA CI8PQ UT WOS:000355034600003 PM 25812832 ER PT J AU Brooks, JP McLaughlin, MR Adeli, A Miles, DM AF Brooks, J. P. McLaughlin, M. R. Adeli, A. Miles, D. M. TI Pathogen re-colonization of in-house composted and noncomposted broiler litter SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler; colonization; litter; poultry; Salmonella; Listeria; Campylobacter ID SALMONELLA-ENTERICA; INDICATORS; BIOSOLIDS; REGROWTH; AMMONIA AB In-house litter composting has been reintroduced to the industry and shown to reduce bacteria by as much as 2 orders of magnitude. Other industries have demonstrated that pathogens can recolonize a waste-residual when microbial competition has been reduced or inhibited following composting. Poultry growers, in the process of shifting to in-house composting for pathogen control, should be aware of this potential problem. A laboratory microcosm study investigated pathogenic bacteria recolonization into composted and noncomposted broiler litter over a simulated broiler grow-out cycle. Objectives were to: 1) determine colonization potential for zoonotic and poultry bacterial pathogens, 2) identify beneficial bacteria which reduce pathogen recolonization, and 3) identify the effects of ammonia on pathogen recolonization. Composted broiler litter allowed Listeria and Campylobacter to colonize within the first 2 wk of the grow-out period while noncomposted litter resisted colonization. Colonization was nearly identical by the end of the grow-out period, and showed that bacterial pathogens had essentially been overtaken by commensal or normal bacteria. 16S rRNA libraries demonstrated reductions in Proteobacteria associated with composted litter (48 vs. 16%), which may indicate that this phylum occupies a niche which zoonotic pathogens prefer to occupy. Ammonia generation neither inhibited nor promoted bacterial colonization, as levels were high for both litter treatments. This study neither suggests nor condemns the continued use of this cost-effective, litter-treatment process; findings suggest that while the beneficial microbial population was initially reduced, it quickly recovered and pathogen colonization was neither enhanced nor inhibited because of this. This study demonstrates that the recently adopted in-house composting process may continue to be used, provided poultry health gains continue to be seen. C1 [Brooks, J. P.; McLaughlin, M. R.; Adeli, A.; Miles, D. M.] ARS, USDA, Mississippi State, MS 39762 USA. RP Brooks, JP (reprint author), ARS, USDA, Mississippi State, MS 39762 USA. EM john.brooks@ars.usda.gov FU U.S. Poultry and Egg Association [662] FX This study was funded by the U.S. Poultry and Egg Association Project #662. The authors would like to acknowledge Cindy Smith and Renotta Smith for their technical aid in processing samples. NR 21 TC 1 Z9 1 U1 3 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2015 VL 24 IS 2 BP 157 EP 167 DI 10.3382/japr/pfv013 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CJ0DO UT WOS:000355144000007 ER PT J AU Berrang, ME Cox, NA Meinersmann, RJ Oakley, BB Line, JE AF Berrang, Mark E. Cox, Nelson A. Meinersmann, Richard J. Oakley, Brian B. Line, J. Eric TI Detection of Campylobacter in 100 commercial flocks-Evaluation of plating media and filtration method SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler; Campylobacter; culture methods; plating media; filter method ID BROILER CARCASSES; AGAR PLATES; SPP.; CONTAMINATION; ENUMERATION; PREVALENCE; SAMPLES; COLONIZATION; POSTCHILL; RECOVERY AB Campylobacter is a natural member of the gut microflora in many commercial broilers and as such can become a contaminant on edible surfaces during processing. Culturing gut contents or feces can be a means to determine flock status prior to live-haul. The wide variety of non-Campylobacter background bacteria in these complex samples contaminates growth media and can make it very difficult to isolate Campylobacter. Over the course of 17 months, we cultured cecal contents from 100 different broiler flocks. For the last 50 flocks, we tested 3 selective plating media with and without the additional selection of a 0.45-mu m filter for detection of Campylobacter from cecal contents. Furthermore, from the last 50 flocks we also collected and cultured carcass rinse samples. Growth media tested included: Campy-Cefex Agar, Campy-Line Agar, and RF-Campylobacter jejuni/coli agar. About half (52%) of the 100 tested flocks were positive for Campylobacter; positive flocks were detected during each month of the year. Overall, the Campylobacter status of cecal contents from one carcass was predictive of the status of a carcass rinse from the same flock. Placing a complex sample such as cecal contents onto a 0.45-mu m filter laid on top of the plating medium improved the detection of Campylobacter by eliminating non-Campylobacter background colonies. All media allowed for detection of Campylobacter from less complex carcass rinse samples without filtering. However, Campy-Cefex agar had higher numbers of competing bacterial colonies than did Campy-Line agar or RF-Campylobacter jejuni/coli agar. C1 [Berrang, Mark E.; Cox, Nelson A.; Meinersmann, Richard J.; Oakley, Brian B.; Line, J. Eric] ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. RP Berrang, ME (reprint author), ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. EM mark.berrang@ars.usda.gov NR 20 TC 2 Z9 2 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2015 VL 24 IS 2 BP 240 EP 245 DI 10.3382/japr/pfv022 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CJ0DO UT WOS:000355144000016 ER PT J AU Bratton, MR Martin, EC Elliott, S Rhodes, LV Collins-Burow, BM McLachlan, JA Wiese, TE Boue, SM Burow, ME AF Bratton, Melyssa R. Martin, Elizabeth C. Elliott, Steven Rhodes, Lyndsay V. Collins-Burow, Bridgette M. McLachlan, John A. Wiese, Thomas E. Boue, Stephen M. Burow, Matthew E. TI Glyceollin, a novel regulator of mTOR/p70S6 in estrogen receptor positive breast cancer SO JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Glyceollin; Endocrine therapy; p70S6; Estrogen receptor (ER); mTOR; Anti-estrogen; Kinase signaling ID ACTIVATED PROTEIN-KINASE; TRANSCRIPTIONAL ACTIVATION; ANTIESTROGEN RESISTANCE; IN-VITRO; PHOSPHORYLATION; ALPHA; CELLS; INHIBITION; MODULATION; MECHANISMS AB An estimated 70% of breast cancer tumors utilize estrogen receptor (ER) signaling to maintain tumorigenesis and targeting of the estrogen receptor is a common method of treatment for these tumor types. However, ER-positive (+) breast cancers often acquire drug resistant or altered ER activity in response to anti-estrogens. Here we demonstrate glyceollin, an activated soy compound, has antiestrogen effects in breast cancers. We demonstrate through estrogen response element luciferase and phosphorylation-ER mutants that the effects of glyceollin arise from mechanisms distinct from conventional endocrine therapies. We show that glyceollin suppresses estrogen response element activity; however, it does not affect ER-alpha (a) phosphorylation levels. Additionally we show that glyceollin suppresses the phosphorylation of proteins known to crosstalk with ER signaling, specifically we demonstrate an inhibition of ribosomal protein S6 kinase, 70 kDa (p70S6) phosphorylation following glyceollin treatment. Our data suggests a mechanism for glyceollin inhibition of ERa through the induced suppression of p70S6 and demonstrates novel mechanisms for ER inhibition. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Martin, Elizabeth C.; Elliott, Steven; Rhodes, Lyndsay V.; Collins-Burow, Bridgette M.; McLachlan, John A.; Burow, Matthew E.] Tulane Univ, Dept Med, Sect Hematol & Med Oncol, New Orleans, LA 70118 USA. [McLachlan, John A.; Burow, Matthew E.] Tulane Univ, Dept Pharmacol, New Orleans, LA 70118 USA. [McLachlan, John A.; Burow, Matthew E.] Tulane Univ, Ctr Bioenvironm Res, New Orleans, LA 70118 USA. [Bratton, Melyssa R.; Wiese, Thomas E.] Xavier Univ, Coll Pharm, Div Basic Pharmaceut Sci, New Orleans, LA 70125 USA. [Boue, Stephen M.] USDA, So Reg Res Ctr, New Orleans, LA 70179 USA. RP Burow, ME (reprint author), Tulane Univ, Dept Med, Sect Hematol & Med Oncol, New Orleans, LA 70118 USA. EM mburow@tulane.edu FU Office of Naval Research [N00014-16-1-1136]; National Institutes of Health [CA125806]; COBRE [5P20GM103518]; U.S. Department of Agriculture [58-6435-7-019]; Department of Defense [W81XWH-11-1-0105]; NIMHD RCMI program 382 [8G12MD007595] FX This research was supported by The Office of Naval Research N00014-16-1-1136 and by National Institutes of Health- CA125806 (ME Burow) and COBRE 5P20GM103518 (BM Collins-Burow), U.S. Department of Agriculture 58-6435-7-019 (ME Burow, JA McLachlan, and SM Boue), Department of Defense Award W81XWH-11-1-0105 and NIMHD RCMI program 382 through Grant 8G12MD007595 (TE Weise). NR 36 TC 1 Z9 1 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-0760 J9 J STEROID BIOCHEM JI J. Steroid Biochem. Mol. Biol. PD JUN PY 2015 VL 150 BP 17 EP 23 DI 10.1016/j.jsbmb.2014.12.014 PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA CJ3GB UT WOS:000355370300003 PM 25771071 ER PT J AU Tagtow, A Haven, J Maniscalco, S Bard, S Larson, J AF Tagtow, Angie Haven, Jackie Maniscalco, Shelley Bard, Sasha Larson, Jessica TI Behind the Scenes at the US Department of Agriculture SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Tagtow, Angie; Haven, Jackie; Maniscalco, Shelley; Bard, Sasha; Larson, Jessica] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. RP Tagtow, A (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD JUN PY 2015 VL 115 IS 6 BP 868 EP 870 DI 10.1016/j.jand.2015.04.008 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ0BM UT WOS:000355137100002 PM 26025247 ER PT J AU Johnson, RK Lichtenstein, AH Kris-Etherton, PM Carson, AS Pappas, A Rupp, L Vafiadis, DK AF Johnson, Rachel K. Lichtenstein, Alice H. Kris-Etherton, Penny M. Carson, Ann S. Pappas, Antigoni Rupp, Linda Vafiadis, Dorothea K. TI Enhanced and Updated American Heart Association Heart-Check Front-of-Package Symbol: Efforts to Help Consumers Identify Healthier Food Choices SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article C1 [Johnson, Rachel K.] Univ Vermont, Dept Nutr & Food Sci, Burlington, VT 05405 USA. [Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Kris-Etherton, Penny M.] Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. [Carson, Ann S.] Univ Texas SW Med Ctr Dallas, Dept Clin Nutr, Dallas, TX 75390 USA. [Pappas, Antigoni; Rupp, Linda] Amer Heart Assoc, Dallas, TX USA. [Vafiadis, Dorothea K.] Amer Heart Assoc, Washington, DC USA. RP Johnson, RK (reprint author), Univ Vermont, Dept Nutr & Food Sci, Burlington, VT 05405 USA. FU American Heart Association FX This research was funded by the American Heart Association. NR 8 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD JUN PY 2015 VL 115 IS 6 BP 876 EP 884 DI 10.1016/j.jand.2015.02.008 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ0BM UT WOS:000355137100004 PM 25840936 ER PT J AU Polley, HW Derner, JD Jackson, RB Gill, RA Procter, AC Fay, PA AF Polley, H. Wayne Derner, Justin D. Jackson, Robert B. Gill, Richard A. Procter, Andrew C. Fay, Philip A. TI Plant community change mediates the response of foliar delta N-15 to CO2 enrichment in mesic grasslands SO OECOLOGIA LA English DT Article DE Isotope; Plant productivity; Soil carbon; Soil type; Tallgrass prairie ID SOIL ORGANIC-MATTER; ATMOSPHERIC CARBON-DIOXIDE; N-15 NATURAL-ABUNDANCE; ELEVATED CO2; NITROGEN AVAILABILITY; SPECIES COMPOSITION; MYCORRHIZAL FUNGI; PRODUCTIVITY; SUBAMBIENT; VEGETATION AB Rising atmospheric CO2 concentration may change the isotopic signature of plant N by altering plant and microbial processes involved in the N cycle. CO2 may increase leaf delta N-15 by increasing plant community productivity, C input to soil, and, ultimately, microbial mineralization of old, N-15-enriched organic matter. We predicted that CO2 would increase aboveground productivity (ANPP; g biomass m(-2)) and foliar delta N-15 values of two grassland communities in Texas, USA: (1) a pasture dominated by a C-4 exotic grass, and (2) assemblages of tallgrass prairie species, the latter grown on clay, sandy loam, and silty clay soils. Grasslands were exposed in separate experiments to a pre-industrial to elevated CO2 gradient for 4 years. CO2 stimulated ANPP of pasture and of prairie assemblages on each of the three soils, but increased leaf delta N-15 only for prairie plants on a silty clay. delta N-15 increased linearly as mineral-associated soil C declined on the silty clay. Mineral-associated C declined as ANPP increased. Structural equation modeling indicted that CO2 increased ANPP partly by favoring a tallgrass (Sorghastrum nutans) over a mid-grass species (Bouteloua curtipendula). CO2 may have increased foliar delta N-15 on the silty clay by reducing fractionation during N uptake and assimilation. However, we interpret the soil-specific, delta N-15-CO2 response as resulting from increased ANPP that stimulated mineralization from recalcitrant organic matter. By contrast, CO2 favored a forb species (Solanum dimidiatum) with higher delta N-15 than the dominant grass (Bothriochloa ischaemum) in pasture. CO2 enrichment changed grassland delta N-15 by shifting species relative abundances. C1 [Polley, H. Wayne; Fay, Philip A.] ARS, Grassland Soil & Water Res Lab, USDA, Temple, TX 76502 USA. [Derner, Justin D.] ARS, High Plains Grasslands Res Stn, USDA, Cheyenne, WY 82009 USA. [Jackson, Robert B.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Jackson, Robert B.] Stanford Univ, Sch Earth Sci, Stanford, CA 94305 USA. [Gill, Richard A.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Procter, Andrew C.] US EPA, Durham, NC 27709 USA. RP Polley, HW (reprint author), ARS, Grassland Soil & Water Res Lab, USDA, Temple, TX 76502 USA. EM wayne.polley@ars.usda.gov FU U.S. Department of Energy [ER64242] FX Chris Kolodziejczyk operated CO2 chambers. Field and laboratory assistance from Anne Gibson, Katherine Jones, Chris Kolodziejczyk, Alicia Naranjo, Kyle Tiner, and numerous students was invaluable. Joseph Craine and Kevin Mueller provided insightful suggestions that improved the manuscript. R.J. acknowledges support from the U.S. Department of Energy (Program in Ecosystem Research #ER64242). Mention of trade names or commercial products does not imply endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 1 Z9 1 U1 5 U2 34 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD JUN PY 2015 VL 178 IS 2 BP 591 EP 601 DI 10.1007/s00442-015-3221-x PG 11 WC Ecology SC Environmental Sciences & Ecology GA CI6VF UT WOS:000354900700025 PM 25604918 ER PT J AU Verma, SK Ajzenberg, D Rivera-Sanchez, A Su, C Dubey, JP AF Verma, S. K. Ajzenberg, D. Rivera-Sanchez, A. Su, C. Dubey, J. P. TI Genetic characterization of Toxoplasma gondii isolates from Portugal, Austria and Israel reveals higher genetic variability within the type II lineage SO PARASITOLOGY LA English DT Article DE Toxoplasma gondii; genotyping; PCR-RFLP; microsatellite analysis; Portugal; Israel; Austria ID FREE-RANGE CHICKENS; HIGH PREVALENCE; IMMUNOCOMPROMISED PATIENTS; CONGENITAL TOXOPLASMOSIS; CLINICAL FINDINGS; GENOTYPE; VIRULENCE; ANIMALS; IDENTIFICATION; RECOMBINATION AB This study compared genetic diversity of Toxoplasma gondii isolates from Portugal, Austria and Israel. For this, we genotyped 90 T. gondii isolates (16 from Portugal, 67 from Austria and 7 from Israel) using 10 nested PCR-restriction length polymorphism (RFLP) genetic markers and 15 microsatellite (MS) markers. By PCR-RFLP typing, 7 isolates from Portugal chickens were identified as type II (ToxoDB #1 or #3), 4 were type III (ToxoDB #2) and the remaining 4 isolates have unique genotype pattern were designated as ToxoDB #254. One mouse virulent isolate from a bovine fetus (Bos taurus) in Portugal was type I (ToxoDB #10) at all loci and designated as TgCowPr1. All 67 isolates from Austria and 7 from Israel were type II (ToxoDB #1 or #3). By MS typing, many additional genetic variations were revealed among the type II and type III isolates. Phylogenetic analysis showed that isolates from the same geographical locations tend to cluster together, and there is little overlapping of genotypes among different locations. This study demonstrated that the MS markers can provide higher discriminatory power to reveal association of genotypes with geographical locations. Future studies of the type II strains in Europe by these MS markers will be useful to reveal transmission patterns of the parasite. C1 [Verma, S. K.; Dubey, J. P.] USDA ARS, BARC, APDL, Beltsville, MD 20705 USA. [Ajzenberg, D.] Univ Dupuytren, Ctr Hosp, CNR, Toxoplasmose Toxoplasma Biol Resource Ctr BRC, F-87042 Limoges, France. [Ajzenberg, D.] Univ Limoges, Fac Med, INSERM, Lab Parasitol Mycol,UMR 1094,Neuroepidemiol Trop, F-87025 Limoges, France. [Rivera-Sanchez, A.; Su, C.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), USDA ARS, BARC, APDL, E Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov NR 55 TC 1 Z9 1 U1 2 U2 14 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD JUN PY 2015 VL 142 IS 7 BP 948 EP 957 DI 10.1017/S0031182015000050 PG 10 WC Parasitology SC Parasitology GA CJ1UN UT WOS:000355270200010 PM 25677825 ER PT J AU Makkar, S Rath, NC Packialakshmi, B Huff, WE Huff, GR AF Makkar, S. Rath, N. C. Packialakshmi, B. Huff, W. E. Huff, G. R. TI Nutritional effects of egg shell membrane supplements on chicken performance and immunity SO POULTRY SCIENCE LA English DT Article DE egg shell membrane; chicken; growth; immunity; stress ID PEPTIDES; PROTEINS; ANTIBIOTICS; LACTOFERRIN; EGGSHELL; GROWTH; ALTERNATIVES; MATURATION; LYSOZYME; DISEASE AB Eggshell membranes (ESM) contain a variety of proteins and peptides which help in the development of embryo and provide protection to it. Many of the peptides and proteins associated with ESM have antimicrobial, immune-modulatory, and adjuvant properties. We hypothesized that the membrane byproducts from egg, provided as posthatch nutritional supplements to chickens, may improve their performance and immunity. To explore its effect, we fed 3 groups of broiler chicks with feed containing 0, 0.2, and 0.4% ESM from d 1 posthatch through 14 d and regular feed thereafter. The birds were individually weighed at the onset of the study and at weekly intervals until the termination at third wk when they were bled and euthanized. The relative weights of liver, spleen, bursa, and heart, hematology profiles, and clinical chemistry variables including serum IgM, IgG, and corticosterone concentrations were measured. The chickens in the ESM treated groups showed a statistically significant increase in BW with no impact on relative organ weights. Compared with controls, the WBC and lymphocyte percentage increased in chickens fed 0.4% ESM whereas the monocyte percentage decreased at both levels of ESM. Except for the serum protein which increased in ESM fed birds no other metabolic clinical chemistry variables showed any significant change. Both IgM and IgG(Y) levels were elevated and corticosterone levels reduced in chickens fed ESM supplemented diets. Our results suggest that ESM supplements during the early phases of growth may improve immunity and stress variables, and enhance their growth performance without any detrimental effect on other physiological parameters. C1 [Makkar, S.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Rath, N. C.; Huff, W. E.; Huff, G. R.] Univ Arkansas, USDA ARS, Fayetteville, AR 72701 USA. [Packialakshmi, B.] Univ Arkansas, Poultry Sci Ctr, Cell & Mol Biol Program, Fayetteville, AR 72701 USA. RP Rath, NC (reprint author), Univ Arkansas, USDA ARS, Fayetteville, AR 72701 USA. EM narayan.rath@ars.usda.gov NR 27 TC 3 Z9 3 U1 1 U2 15 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN PY 2015 VL 94 IS 6 BP 1184 EP 1189 DI 10.3382/ps/pev098 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CJ0TA UT WOS:000355189700012 PM 25840966 ER PT J AU Napper, S Dadgar, S Arsenault, RJ Trost, B Scruten, E Kusalik, A Shand, P AF Napper, Scott Dadgar, Samira Arsenault, Ryan J. Trost, Brett Scruten, Erin Kusalik, Anthony Shand, Phyllis TI Induction of tissue- and stressor-specific kinomic responses in chickens exposed to hot and cold stresses SO POULTRY SCIENCE LA English DT Article DE kinome; peptide array; thermal stress; chickens ID ACTIVATED PROTEIN-KINASE; CHRONIC HEAT EXPOSURE; BREAST MEAT QUALITY; BROILER-CHICKENS; SKELETAL-MUSCLE; GROWTH-PERFORMANCE; FATTY-ACID; TEMPERATURE; METABOLISM; PARAMETERS AB Defining cellular responses at the level of global cellular kinase (kinome) activity is a powerful approach to deciphering complex biology and identifying biomarkers. Here we report on the development of a chicken-specific peptide array and its application to characterizing kinome responses within the breast (pectoralis major) and thigh (iliotibialis) muscles of poultry subject to temperature stress to mimic conditions experienced by birds during commercial transport. Breast and thigh muscles exhibited unique kinome profiles, highlighting the distinct nature of these tissues. Against these distinct backgrounds, tissue- and temperature-specific kinome responses were observed. In breast, both cold and hot stresses activated calcium-dependent metabolic adaptations. Also within breast, but specific to cold stress, was the activation of ErbB signaling as well as dynamic patterns of phosphorylation of AMPK, a key regulatory enzyme of metabolism. In thigh, cold stress induced responses suggestive of the occurrence of tissue damage, including activation of innate immune signaling pathways and tissue repair pathways (TGF-beta). In contrast, heat stress in thigh activated pathways associated with protein and fat metabolism through adipocytokine and mammalian target of rapamycin (mTOR) signaling. Defining the responses of these tissues to these stresses through conventional markers of pH, glycolytic potential, and meat quality offered a similar conclusion of the tissue- and stressor-specific responses, validating the kinome results. Collectively, the results of this study highlight the unique cellular responses of breast and thigh tissues to heat and cold stresses and may offer insight into the unique susceptibilities, as well as functional consequences, of these tissues to thermal stress. C1 [Napper, Scott; Scruten, Erin] Univ Saskatchewan, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada. [Napper, Scott] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5E5, Canada. [Trost, Brett; Kusalik, Anthony] Univ Saskatchewan, Dept Comp Sci, Saskatoon, SK S7N 5C9, Canada. [Dadgar, Samira; Shand, Phyllis] Univ Saskatchewan, Dept Food & Bioprod Sci, Saskatoon, SK S7N 5A8, Canada. [Arsenault, Ryan J.] USDA ARS, SPARC, College Stn, TX 77845 USA. RP Napper, S (reprint author), Univ Saskatchewan, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada. EM scott.napper@usask.ca RI Trost, Brett/K-4127-2016 OI Trost, Brett/0000-0003-4863-7273 FU Natural Science and Engineering Research Council; Agriculture and Agri-Food Canada; Saskatchewan Chicken Industry Development Fund; Alberta Farm Animal Care; Chicken Farmers of Saskatchewan; Alberta Chicken Producers; Canadian Poultry Research Council; Poultry Industry Council; Sofina Foods Inc. (Lilydale) FX The study was supported by funds provided by the Natural Science and Engineering Research Council, Agriculture and Agri-Food Canada, Saskatchewan Chicken Industry Development Fund, Alberta Farm Animal Care, Chicken Farmers of Saskatchewan, Alberta Chicken Producers, Canadian Poultry Research Council, Poultry Industry Council, and Sofina Foods Inc. (Lilydale). Special thanks to T. Crowe, M. Strawford, and the staff of the Poultry Research Center at the University of Saskatchewan for their support and participation in data collection. NR 62 TC 2 Z9 2 U1 3 U2 19 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN PY 2015 VL 94 IS 6 BP 1333 EP 1345 DI 10.3382/ps/pev046 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA CJ0TA UT WOS:000355189700027 PM 25838314 ER PT J AU Ploeg, MV Dutko, P Breneman, V AF Ploeg, Michele Ver Dutko, Paula Breneman, Vince TI Measuring Food Access and Food Deserts for Policy Purposes SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Policy targeting; food deserts; food access; poverty ID ENVIRONMENTS; PRICES AB Policymakers have dedicated increasing attention to whether Americans have access to healthful food. As a result, various methods for measuring food store access at the national level have been developed to identify areas that lack access. However, these methods face definitional, data, and methodological limitations. The focus on neighborhoods instead of individuals underestimates the barriers that some individuals face in accessing healthy food, and overestimates the problem in other neighborhoods. This paper reviews and critiques currently available national-level measures of food access. While multiple measures of food access are needed to understand the problem, we recommend greater attention be paid to individual measures of food store access. C1 [Ploeg, Michele Ver] USDA, Econ Res Serv, Washington, DC 20250 USA. [Dutko, Paula] Univ Florida, Food & Resource Econ Dept, Gainesville, FL 32611 USA. [Breneman, Vince] USDA, Res Support Branch, Washington, DC USA. RP Ploeg, MV (reprint author), USDA, Econ Res Serv, Washington, DC 20250 USA. EM Sverploeg@ers.usda.gov NR 36 TC 1 Z9 1 U1 5 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD JUN PY 2015 VL 37 IS 2 BP 205 EP 225 DI 10.1093/aepp/ppu035 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA CI4DH UT WOS:000354696400002 ER PT J AU Ahearn, MC Williamson, JM Black, N AF Ahearn, Mary Clare Williamson, James M. Black, Nyesha TI Implications of Health Care Reform for Farm Businesses and Families SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Affordable Care Act; farm employers; health insurance; hired farm labor; off-farm work ID INSURANCE; MASSACHUSETTS; DISPARITIES; HOUSEHOLDS; COVERAGE; ACCESS AB The Affordable Care Act has implications for the source of health insurance for farm households and potentially how much of their time they allocate to off-farm jobs and even the rate at which new operators enter farming. The Act will likely have impacts for the 1% of farms defined to be large employers, which are required to provide coverage for their workers or pay a penalty. While a very small share of all farms, they account for upward of 40% of the production for some commodities. How they adjust their use of farm labor in response to the Affordable Care Act has implications for farm structure. C1 [Ahearn, Mary Clare; Williamson, James M.; Black, Nyesha] USDA, Econ Res Serv, Washington, DC 20250 USA. RP Ahearn, MC (reprint author), USDA, Econ Res Serv, Washington, DC 20250 USA. EM mahearn54@aol.com RI Cabrera, Julio/F-5782-2016 NR 30 TC 1 Z9 1 U1 0 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD JUN PY 2015 VL 37 IS 2 BP 260 EP 286 DI 10.1093/aepp/ppu030 PG 27 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA CI4DH UT WOS:000354696400005 ER PT J AU Kelly, J Canning, P Weersink, A AF Kelly, Jessica Canning, Patrick Weersink, Alfons TI Decomposing the Farmer's Share of the Food Dollar SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Farmer share; Food dollar; Price changes; Input output (IO) analysis ID RETAIL PRICE SPREAD; MARKETING MARGIN; INDUSTRY; OUTPUT AB The Canadian farm share for five crop-based products and seven livestock-based products from 1997 to 2010 is calculated using a supply chain IO analysis. Significant differences exist in farm shares across food commodities with higher farm shares for livestock products and lower farm shares for grain-based products. The decline in the Canadian farm share for food consumed at home is driven in large part by the food purchasing habits of consumers. This paper also addresses the hypothesis that the decline in the Canadian farm share could be partially driven by rising input costs in post-farmgate processes or rising input costs that have greater impact on downstream sectors than primary agricultural producers. Three experiments were conducted to assess the impact of an increase in the cost of corn, energy, and farm labor would have on commodity output prices, farm returns, food expenditure, and farm share. In all three cases, the overall farm share increases, albeit by a small amount, suggesting that these shocks have a larger relative impact on the prices of agricultural commodities than the prices of marketing commodities used in post-farmgate activities. A two-period comparison of these simulations shows that energy (corn and farm labour) price shocks would have had a greater (lower) impact on the farm share in 2007 than 1997. C1 [Kelly, Jessica; Weersink, Alfons] Univ Guelph, Dept Food Agr & Resource Econ, Guelph, ON N1G 2W1, Canada. [Canning, Patrick] USDA, Econ Res Serv, Washington, DC USA. RP Weersink, A (reprint author), Univ Guelph, Dept Food Agr & Resource Econ, Guelph, ON N1G 2W1, Canada. EM aweersin@uoguelph.ca FU Ontario Ministry of Agriculture and Food (OMAF) FX The authors wish to acknowledge the assistance of Ken Nakagawa from Agriculture and Agri-Food Canada (AAFC) and the helpful comments from Ken Hansen, Howard Leathers, Nicolas Rockler, John Cranfield, and Brady Deaton. Financial support from the Ontario Ministry of Agriculture and Food (OMAF) is greatly appreciated. NR 32 TC 1 Z9 1 U1 1 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD JUN PY 2015 VL 37 IS 2 BP 311 EP 331 DI 10.1093/aepp/ppu034 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA CI4DH UT WOS:000354696400007 ER PT J AU Lewicki, KE Huyvaert, KP Piaggio, AJ Diller, LV Franklin, AB AF Lewicki, Krista E. Huyvaert, Kathryn P. Piaggio, Antoinette J. Diller, Lowell V. Franklin, Alan B. TI Effects of barred owl (Strix varia) range expansion on Haemoproteus parasite assemblage dynamics and transmission in barred and northern spotted owls (Strix occidentalis caurina) SO BIOLOGICAL INVASIONS LA English DT Article DE Blood parasite; Enemy Release Hypothesis; Haemoproteus; Invasive species; Parasite Spillback Hypothesis; Dilution Effect Hypothesis; Range expansion ID AVIAN MALARIA PARASITES; BLOOD PARASITES; PREVALENCE; POPULATIONS; DIVERSITY; HEMATOZOA; PATHOGENS; NETWORKS; ECOLOGY; HABITAT AB The range of the barred owl (Strix varia) has expanded westward over the past century and now entirely overlaps the range of the federally threatened northern spotted owl (Strix occidentalis caurina) in the Pacific Northwest. We compared Haemoproteus blood parasite assemblages among northern spotted owls in their native range and barred owls in both their native and invasive ranges to evaluate predictions of five hypotheses about parasites and biological invasions: (1) Enemy Release, where hosts benefit from a loss of parasites in their invasive range, (2) Enemy of My Enemy, where invasive hosts introduce parasites to naive native hosts, (3) Parasite Spillback, where invasive hosts act as a new reservoir to native parasites, (4) Increased Susceptibility, where native hosts introduce parasites to naive invasive hosts, and (5) Dilution Effect, where invasive species act as poor hosts to native parasites and decrease the density of potential hosts in their invasive range. We used haplotype network analyses to identify one haplotype common to both owl species throughout North America, three more haplotypes that appeared to be isolated to the barred owl's historic range, and a fifth haplotype that was only found in California. Based on infection status and parasite diversity in eastern and western barred owl populations, we found strong support for the Enemy Release Hypothesis. Northern spotted owls had higher parasite diversity and probability of infection than sympatric barred owls, offering some support for the Parasite Spillback and Dilution Effect Hypotheses. Overall, this study demonstrates the complexity of host-parasite relationships and highlights some of the ways in which species' range expansions may alter such relationships among both invasive and native hosts. C1 [Lewicki, Krista E.; Huyvaert, Kathryn P.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Piaggio, Antoinette J.; Franklin, Alan B.] USDA APHIS WS Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Diller, Lowell V.] Green Diamond Resource Co, Korbel, CA 95550 USA. RP Franklin, AB (reprint author), USDA APHIS WS Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM krista.lewicki@gmail.com; kate.huyvaert@colostate.edu; Toni.J.Piaggio@aphis.usda.gov; LDiller@greendiamond.com; Alan.B.Franklin@aphis.usda.gov FU Colorado State University Institutional Animal Care and Use Committee protocol [10-1818A]; U.S.D.A. Forest Service Region 5 [11-CS-11052007-319]; Colorado State University FX We thank Peter Carlson and Jeremy Rockweit of Klamath Biological Research Station; Mark Higley and the Hoopa Valley Tribe; Green Diamond Resource Company; Robert Feamster and Sierra Pacific Industries; and Laurie Clark and the National Council for Air and Stream Improvement, Inc., for allowing us to conduct research on their lands and for assistance in collecting western owl samples. The Avian Conservation Center (South Carolina), Wildcare Foundation (Oklahoma), Avian Haven (Maine), Carolina Raptor Center (North Carolina), Audubon of Florida (Florida), The Raptor Center (Minnesota), Tri-State Bird Rescue & Research (Delaware), and Alabama Raptor Center (Alabama) collected all the eastern barred owl samples analyzed in this study. Additional field and laboratory assistance came from Constanza Toro, Annie Kellner, Matthew Hopken, Nikki Crider, and Jeremy Dertien. Dr. Thomas Gidlewski provided access to his microscope; Nic Berrong assisted in installing and navigating the i-Solution Lite software; and Drs. Ellen Martinsen and Robert Ricklefs provided positive control samples. Finally, Drs. Liba Pejchar, Brian Foy, Ken Burnham, and Ann Hess assisted in study design and analysis and 2 anonymous reviewers provided helpful comments and suggestions that greatly improved our manuscript. This work was conducted under the auspices of the Colorado State University Institutional Animal Care and Use Committee protocol # 10-1818A. Funding and additional support for this project was provided by the U.S.D.A. Forest Service Region 5 contract 11-CS-11052007-319 and Colorado State University. NR 58 TC 2 Z9 2 U1 4 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD JUN PY 2015 VL 17 IS 6 BP 1713 EP 1727 DI 10.1007/s10530-014-0828-5 PG 15 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CH9RJ UT WOS:000354373500012 ER PT J AU Pitt, WC Sugihara, RT Berentsen, AR AF Pitt, William C. Sugihara, Robert T. Berentsen, Are R. TI Effect of travel distance, home range, and bait on the management of small Indian mongooses, Herpestes auropunctatus SO BIOLOGICAL INVASIONS LA English DT Article DE Bait attractiveness; Control; Foraging distance; Hawaii; Management ID CARNIVORA-VIVERRIDAE; ANTHROPOGENIC FOOD; ORAL TOXICITY; RAIN-FOREST; WEST-INDIES; JAVANICUS; MAMMALS; ISLAND; AMINOPROPIOPHENONE; VACCINATION AB Selection of preferred baits to attract mongooses to traps and other control devices is paramount in the effective management and control of this invasive predatory mammal. We examined the attractiveness of selected food items as baits to free-ranging mongooses in field trials at two different habitats on the island of Hawaii. We utilized radio telemetry to calculate mongoose home range and population density estimates. We implanted microchips to remotely identify and record visitations by mongooses to the candidate baits and investigated bait visitation rates, bait attraction distances, and bait discovery times. Mongooses in this study foraged over a wide area and readily investigated the various novel food baits, with fish, beef and egg-baited stations eliciting higher first and revisits over multiple days. We radio collared 34 mongooses. Overall mean home range estimates were 21.9 and 28.8 ha and did not differ between the two study sites (F = 2.12, p = 0.156), although overall male mongooses had larger home ranges than females (F = 22.92, df = 1, p < 0.0001). Extensive overlapping home ranges were recorded among individual mongooses, regardless of gender. Male mongooses were attracted from a greater distance to selected baits as compared to females (F = 15.80, df = 1, p = 0.0004) although females visited more bait stations than males at each site (F = 11.26, df = 1, p = 0.002 and F = 6.90, df = 1, p = 0.017). Baits were usually discovered within 24-30 h of exposure. Based on time to first bait discovery, no differences were found among percent of food stations visited among bait types at either site (F = 0.93, df = 4, p = 0.463 and F = 0.40, df = 3, p = 0.756). The results of this study provide insights on mongoose foraging ecology in Hawaii and the attractiveness of food baits used in developing effective control strategies in detecting and trapping mongooses in newly established areas as well as reducing or eradicating populations in native species habitat impacted by mongooses. C1 [Pitt, William C.; Sugihara, Robert T.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr,Hawaii Field Stn, Hilo, HI 96720 USA. [Berentsen, Are R.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, Hilo, HI 96720 USA. RP Pitt, WC (reprint author), Smithsonian Conservat Biol Inst, Natl Zool Pk, Front Royal, VA 22630 USA. EM pittw@si.edu FU U. S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office FX We are grateful for the support and assistance provided by the U. S. Fish and Wildlife Service, Pacific Islands Fish and Wildlife Office. Capture, handling and marking of mongooses was performed with approval of the National Wildlife Research Center's Institutional Animal Care and Use Committee February 25, 2005 under QA-1255. We thank Michael W. Fall and Catherine E. Swift for assistance with literature review and manuscript preparation. Special thanks to two anonymous reviewers for helpful and insightful comments on an earlier draft of this manuscript. NR 52 TC 3 Z9 3 U1 8 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD JUN PY 2015 VL 17 IS 6 BP 1743 EP 1759 DI 10.1007/s10530-014-0831-x PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CH9RJ UT WOS:000354373500014 ER PT J AU Chen, LH Wang, QQ Hirth, K Baez, C Agarwal, UP Zhu, JY AF Chen, Liheng Wang, Qianqian Hirth, Kolby Baez, Carlos Agarwal, Umesh P. Zhu, J. Y. TI Tailoring the yield and characteristics of wood cellulose nanocrystals (CNC) using concentrated acid hydrolysis SO CELLULOSE LA English DT Article DE Cellulose nanocrystals (CNC); Crystallinity; Crystal length and morphology; Surface charge; CNC yield optimization; Concentrated acid hydrolysis ID CHIRAL NEMATIC SUSPENSIONS; MICROCRYSTALLINE CELLULOSE; CRYSTALLINITY; EXTRACTION; STABILITY AB Cellulose nanocrystals (CNC) have recently received much attention in the global scientific community for their unique mechanical and optical properties. Here, we conducted the first detailed exploration of the basic properties of CNC, such as morphology, crystallinity, degree of sulfation and yield, as a function of production condition variables. The rapid cellulose depolymerization and sulfation reactions under concentrated acid concentrations of around 60 wt% resulted in a very narrow operating window for CNC production. We found that CNC yields as high as 70 wt% from a bleached eucalyptus 'craft pulp with glucan content of 78 wt% can be achieved under a tight range of reaction conditions and that a weighted average length of over 200 nm and sulfur content (a measure of CNC surface charge) between 3 and 10 mg/g can be produced. This study provided critical knowledge for the production of CNC with characteristics tailored for different specific applications, significant to commercialization. C1 [Chen, Liheng] S China Univ Technol, State Key Lab Pulp Paper Engn, Guangzhou 510641, Guangdong, Peoples R China. [Chen, Liheng; Wang, Qianqian; Hirth, Kolby; Baez, Carlos; Agarwal, Umesh P.; Zhu, J. Y.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. [Wang, Qianqian] Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang, Peoples R China. [Zhu, J. Y.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI USA. RP Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, USDA, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM jzhu@fs.fed.us RI Wang, Qianqian/G-1915-2012; Chen, Liheng/E-1985-2014 OI Wang, Qianqian/0000-0003-3514-455X; Chen, Liheng/0000-0001-7501-7972 FU US Forest Service; USDA Agriculture and Food Research Initiative (AFRI) [2011-67009-20056]; Chinese Scholarship Council (CSC), Guangzhou Elite Project of China FX This work was conducted while Chen and Wang were visiting Ph.D students at the US Forest Service, Forest Products Laboratory (FPL), Madison, WI, and on official government time of Zhu, Baez, and Hirth. This work was supported by US Forest Service. Financial support included the USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant (No. 2011-67009-20056), Chinese Scholarship Council (CSC), Guangzhou Elite Project of China. Funding from these programs made the visiting appointments of Chen and Wang at FPL possible. We also would like to acknowledge Drs. Ulrich Baxa of Nanotechnology Characterization Laboratory, Frederick National Laboratory for Cancer Research and Robin Harris of Johannes Gutenberg-University, Germany, for their insightful discussion on TEM images. NR 32 TC 24 Z9 24 U1 12 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD JUN PY 2015 VL 22 IS 3 BP 1753 EP 1762 DI 10.1007/s10570-015-0615-1 PG 10 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA CH7CK UT WOS:000354193000022 ER PT J AU Stambaugh, MC Varner, JM Noss, RF Dey, DC Christensen, NL Baldwin, RF Guyette, RP Hanberry, BB Harper, CA Lindblom, SG Waldrop, TA AF Stambaugh, Michael C. Varner, J. Morgan Noss, Reed F. Dey, Daniel C. Christensen, Norman L. Baldwin, Robert F. Guyette, Richard P. Hanberry, Brice B. Harper, Craig A. Lindblom, Sam G. Waldrop, Thomas A. TI Clarifying the role of fire in the deciduous forests of eastern North America: reply to Matlack SO CONSERVATION BIOLOGY LA English DT Article ID WHITE-TAILED DEER; MISSOURI OZARKS; NEW-ENGLAND; HARDWOOD FORESTS; SOIL CHARCOAL; OAK; USA; HISTORY; MESOPHICATION; RESTORATION C1 [Stambaugh, Michael C.; Guyette, Richard P.; Hanberry, Brice B.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Varner, J. Morgan] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Noss, Reed F.] Univ Cent Florida, Dept Biol, Orlando, FL 32816 USA. [Dey, Daniel C.] Univ Missouri, USDA, US Forest Serv, No Res Stn, Columbia, MO 65211 USA. [Christensen, Norman L.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Baldwin, Robert F.] Clemson Univ, Dept Forestry & Conservat, Clemson, SC 29634 USA. [Harper, Craig A.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Ellington Plant Sci 280, Knoxville, TN 37996 USA. [Lindblom, Sam G.] Virginia Chapter, Nat Conservancy, Charlottesville, VA 22901 USA. [Waldrop, Thomas A.] Clemson Univ, USDA, US Forest Serv, Southern Res Stn, Clemson, SC 29634 USA. RP Stambaugh, MC (reprint author), Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. EM stambaughm@missouri.edu NR 41 TC 10 Z9 10 U1 6 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD JUN PY 2015 VL 29 IS 3 BP 942 EP 946 DI 10.1111/cobi.12473 PG 5 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CI1CD UT WOS:000354477300037 PM 25704587 ER PT J AU Lee, S Jun, TH Michel, AP Mian, MAR AF Lee, Sungwoo Jun, T. H. Michel, Andrew P. Mian, M. A. Rouf TI SNP markers linked to QTL conditioning plant height, lodging, and maturity in soybean SO EUPHYTICA LA English DT Article DE Soybean; SNP markers; QTL; Marker assisted selection ID QUANTITATIVE TRAIT LOCI; GENETIC-LINKAGE MAP; AGRONOMIC TRAITS; PHYTOPHTHORA-SOJAE; INBRED POPULATIONS; PARTIAL RESISTANCE; GROWTH HABIT; PI 567301B; IDENTIFICATION; ALLELES AB Soybean (Glycine max L. Merr.) is a major crop and a leading source of protein meal and edible oil worldwide. Plant height (PHT), lodging (LDG), and days to maturity (MAT) are three important agronomic traits that influence the seed yield of soybean. The objective of this study was to map quantitative trait loci (QTL) for PHT, LDG and MAT using a high density SNP map of a recombinant inbred line soybean mapping population. With single factor analysis of variance six, four, and three QTL were identified across environments for PHT, LDG, and MAT, respectively. Several QTL for each trait were also detected by composite interval mapping with high confidence. Two and one QTL for PHT and LDG, respectively were novel QTL identified in this study. Additionally, most QTL identified in the present study are flanked by two or more SNP markers that are closely linked to each QTL. The SNP markers identified to be closely linked to each QTL in this study are valuable for marker assisted selection (MAS) of the QTL by interested soybean breeding programs. Thus, this study clearly advances the knowledge on genetic controls of plant height, lodging and maturity in soybean, and identifies more efficient and reliable markers for MAS for these traits than those currently exist. C1 [Lee, Sungwoo; Jun, T. H.; Michel, Andrew P.] Ohio State Univ, Dept Entomol, OARDC, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Wooster, OH 44691 USA. RP Mian, MAR (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, 1680 Madison Ave, Wooster, OH 44691 USA. EM rouf.mian@ars.usda.gov FU UDSA-ARS; Ohio State University/Ohio Agricultural Research and Development Center; Ohio Soybean Council FX We thank Tim Mendiola, Keith Freewalt, and Ronald Fioritto for their technical help in this study. We also thank members of the Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD for development and distribution of the Universal Soy Linkage Panel (USLP 1.0) of 1,536 SNP markers used in this study. This study was supported by UDSA-ARS, Ohio State University/Ohio Agricultural Research and Development Center and a Grant from the Ohio Soybean Council. NR 40 TC 4 Z9 4 U1 2 U2 33 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD JUN PY 2015 VL 203 IS 3 BP 521 EP 532 DI 10.1007/s10681-014-1252-8 PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CI5SM UT WOS:000354819700004 ER PT J AU Zhu, Y Wang, HF Hollis, JH Jacques, PF AF Zhu, Yong Wang, Huifen Hollis, James H. Jacques, Paul F. TI The associations between yogurt consumption, diet quality, and metabolic profiles in children in the USA SO EUROPEAN JOURNAL OF NUTRITION LA English DT Article DE NHANES; Yogurt; Glucose; Insulin; Children ID DOSE-RESPONSE METAANALYSIS; HEALTHY EATING INDEX-2005; DAIRY PRODUCT CONSUMPTION; INSULIN-RESISTANCE; RISK-FACTORS; CARDIOVASCULAR RISK; PROBIOTIC YOGURT; BODY-COMPOSITION; NATIONAL-HEALTH; UNITED-STATES AB Recent studies have shown that yogurt consumption was associated with better diet quality and a healthier metabolic profile in adults. However, such associations have not been investigated in children. The present study examined the associations in children using data from a nationally representative survey. Data from 5,124 children aged 2-18 years, who participated in the National Health and Nutrition Examination Survey (NHANES) between 2003 and 2006 in the USA were analyzed. The frequency of yogurt consumption over 12 months was determined using a validated food frequency questionnaire. Diet quality was assessed by the Healthy Eating Index 2005 (HEI-2005) using one 24-HR dietary recall, and metabolic profiles were obtained from the NHANES laboratory data. It was found that only 33.1 % of children consumed yogurt at least once per week (frequent consumers). Adjusting for covariates, frequent consumers had better diet quality than infrequent consumers, as indicated by a higher HEI-2005 total score (P = 0.04). Frequent yogurt consumption was associated with a lower fasting insulin level (P < 0.001), a lower homeostatic model assessment of insulin resistance (P < 0.001), and a higher quantitative insulin sensitivity check index (P = 0.03). However, yogurt consumption was not associated with body weight, fasting glucose, serum lipid profiles, C-reactive protein, and blood pressures (all P > 0.05). These results suggest that frequent yogurt consumption may contribute to improved diet quality and a healthier insulin profile in children. Future longitudinal studies and clinical trials in children are warranted to explore the health benefits of yogurt consumption. C1 [Zhu, Yong] Univ Iowa, Dept Epidemiol, Iowa City, IA USA. [Wang, Huifen; Jacques, Paul F.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Nutr Epidemiol Program, Boston, MA 02111 USA. [Hollis, James H.] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA USA. RP Jacques, PF (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Nutr Epidemiol Program, 711 Washington St, Boston, MA 02111 USA. EM paul.jacques@tufts.edu FU U.S. Department of Agriculture [58-1950-0-014]; Dannon Company, Inc. FX This work was supported by U.S. Department of Agriculture Agreement 58-1950-0-014, and a research Grant from The Dannon Company, Inc. NR 45 TC 3 Z9 3 U1 2 U2 15 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-6207 EI 1436-6215 J9 EUR J NUTR JI Eur. J. Nutr. PD JUN PY 2015 VL 54 IS 4 BP 543 EP 550 DI 10.1007/s00394-014-0735-7 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CI4KG UT WOS:000354716500005 PM 25034879 ER PT J AU Ekanayake, LJ Thavarajah, D Vial, E Schatz, B McGee, R Thavarajah, P AF Ekanayake, Lukshman J. Thavarajah, Dil Vial, Eric Schatz, Blaine McGee, Rebecca Thavarajah, Pushparajah TI Selenium fertilization on lentil (Lens culinaris Medikus) grain yield, seed selenium concentration, and antioxidant activity SO FIELD CROPS RESEARCH LA English DT Article DE Selenium; Lentil; Field experiment; Fertilization ID POTATO SOLANUM-TUBEROSUM; BIOFORTIFICATION; ENVIRONMENT; BRASSICA; GENOTYPE; HEALTH; GROWTH; PLANTS AB Selenium (Se) is an essential element for mammals but has not been considered as an essential element for higher plants. Lentil (Lens culinaris Medik.) is a cool season food legume rich in protein and a range of micronutrients including minerals (iron and zinc), folates, and carotenoids. The objective of this study was to determine the effect of field application of two Se forms on lentil grain yield, seed Se concentration, and antioxidant activity. The experiment was conducted at Carrington Research and Extension Center, ND, USA in 2012 and 2013 with five lentil genotypes and three Se treatments (control, 30 g ha(-1) of selenite, and 30 g ha(-1) of selenate) applied at seeding and at 50% flowering. Application of selenite and selenate increased the lentil grain yield by 10% and 4%, respectively, compared to the control. Seed Se concentration was significantly higher in lentils treated with selenate (1.4 mg kg(-1)) compared to selenite (0.9 mg kg(-1)) and the control (0.6 mg kg(-1)). Selenate increased the antioxidant activity (66% inhibition) of lentils compared to the selenite (64% inhibition) and control (59% inhibition). Overall, Se fertilization is beneficial in increasing lentil seed yield; however selenate will be more beneficial in increasing seed Se concentration. (C) 2015 Elsevier B.V. All rights reserved. C1 [Ekanayake, Lukshman J.; Vial, Eric] N Dakota State Univ, Dept 7640, Fargo, ND 58108 USA. [Thavarajah, Dil] Clemson Univ, Dept Agr & Environm Sci, Poole Agr Ctr 270, Clemson, SC 29634 USA. [Schatz, Blaine] Carrington Res Extens Ctr, Carrington, ND 58421 USA. [McGee, Rebecca] Washington State Univ, USDA, ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. RP Thavarajah, D (reprint author), Clemson Univ, Dept Agr & Environm Sci, Poole Agr Ctr 270, Clemson, SC 29634 USA. EM dthavar@clemson.edu FU Northern Pulse Growers Association, Clemson University, Clemson, SC, USA FX Support for this research was provided by the Northern Pulse Growers Association, Clemson University, Clemson, SC, USA. NR 39 TC 3 Z9 4 U1 2 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD JUN PY 2015 VL 177 BP 9 EP 14 DI 10.1016/j.fcr.2015.03.002 PG 6 WC Agronomy SC Agriculture GA CI8MC UT WOS:000355025400002 ER PT J AU Lee, J Hwang, YS Kang, IK Choung, MG AF Lee, Jinwook Hwang, Young-Sun Kang, In-Kyu Choung, Myoung-Gun TI Metabolic association of lipophilic pigments in the organs of soybean sprouts SO FOOD SCIENCE AND BIOTECHNOLOGY LA English DT Article DE Glycine max (L.) Merr.; chlorophyll a and b; lutein; beta-carotene; principal component analysis ID GLYCINE-SOJA SIEB.; BETA-CAROTENE; LUTEIN CONTENT; SEEDS; MAX; ANTIOXIDANT; ISOFLAVONES; GERMINATION; COMPONENTS; ALFALFA AB Ten soybean sprout varieties were examined for the evaluation of variations in lipophilic pigments, chlorophyll a and b, lutein, and beta-carotene in seeds, whole sprouts, cotyledons, and hypocotyls germinated for 5 days in darkness at 20A degrees C with 80% humidity. Variations in lipophilic pigment contents caused a wide separation of each variety on a principal component analysis (PCA) scores plot, indicating that separation of varieties had wider lipophilic pigment responses from whole sprouts and cotyledons than those from seeds and hypocotyls. Hierarchical cluster analysis indicated that clusters of each organ from 10 varieties corresponded to PCA score plot results. Correlation analysis indicated that there were 4 positive correlations in both whole sprouts and cotyledons, but only 1 or none in hypocotyls and seeds. The responses of lipophilic pigments varied highly depending on variety and organ. C1 [Lee, Jinwook] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Lee, Jinwook; Kang, In-Kyu] Kyungpook Natl Univ, Dept Hort Sci, Daegu 702701, South Korea. [Hwang, Young-Sun; Choung, Myoung-Gun] Kangwon Natl Univ, Dept Herbal Med Resource, Samcheok 245907, Gangwon, South Korea. [Hwang, Young-Sun] Univ Texas Arlington, Dept Biol, Arlington, TX 76019 USA. RP Choung, MG (reprint author), Kangwon Natl Univ, Dept Herbal Med Resource, Samcheok 245907, Gangwon, South Korea. EM cmg7004@kangwon.ac.kr FU Rural Development Administration (RDA), Republic of Korea [PJ 01004602] FX This work was supported by a grant from the National Agenda Programs for Agricultural R&D (PJ 01004602), Rural Development Administration (RDA), Republic of Korea. NR 30 TC 0 Z9 0 U1 4 U2 5 PU KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST PI SEOUL PA #605, KOREA SCI TECHNOL CENT, 635-4 YEOKSAM-DONG, KANGNAM-GU, SEOUL, 135-703, SOUTH KOREA SN 1226-7708 EI 2092-6456 J9 FOOD SCI BIOTECHNOL JI Food Sci. Biotechnol. PD JUN PY 2015 VL 24 IS 3 BP 859 EP 865 DI 10.1007/s10068-015-0111-2 PG 7 WC Food Science & Technology SC Food Science & Technology GA CI6QR UT WOS:000354886300011 ER PT J AU Mama, SK McCurdy, SA Evans, AE Thompson, DI Diamond, PM Lee, RE AF Mama, Scherezade K. McCurdy, Sheryl A. Evans, Alexandra E. Thompson, Deborah I. Diamond, Pamela M. Lee, Rebecca E. TI Using Community Insight to Understand Physical Activity Adoption in Overweight and Obese African American and Hispanic Women: A Qualitative Study SO HEALTH EDUCATION & BEHAVIOR LA English DT Article DE community; exercise; incentive; minority health; participatory research; social environment ID SOCIAL ENVIRONMENTS; MIXED METHODS; EXERCISE; BARRIERS; HEALTH; INTERVENTION; MOTHERS; PROJECT; OTHERS; PARKS AB Ecologic models suggest that multiple levels of influencing factors are important for determining physical activity participation and include individual, social, and environmental factors. The purpose of this qualitative study was to use an ecologic framework to gain a deeper understanding of the underlying behavioral mechanisms that influence physical activity adoption among ethnic minority women. Eighteen African American and Hispanic women completed a 1-hour in-depth interview. Verbatim interview transcripts were analyzed for emergent themes using a constant comparison approach. Women were middle-aged (age M = 43.9 +/- 7.3 years), obese (body mass index M = 35.0 +/- 8.9 kg/m(2)), and of high socioeconomic status (88.9% completed some college or more, 41.2% reported income >$82,600/year). Participants discussed individual factors, including the need for confidence, motivation and time, and emphasized the importance of environmental factors, including their physical neighborhood environments and safety of and accessibility to physical activity resources. Women talked about caretaking for others and social support and how these influenced physical activity behavior. The findings from this study highlight the multilevel, interactive complexities that influence physical activity, emphasizing the need for a more sophisticated, ecologic approach for increasing physical activity adoption and maintenance among ethnic minority women. Community insight gleaned from this study may be used to better understand determinants of physical activity and develop multilevel solutions and programs guided by an ecologic framework to increase physical activity in ethnic minority women. C1 [Mama, Scherezade K.] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA. [Mama, Scherezade K.; Lee, Rebecca E.] Univ Houston, Houston, TX USA. [McCurdy, Sheryl A.; Diamond, Pamela M.] Univ Texas Sch Publ Hlth, Houston, TX USA. [Evans, Alexandra E.] Univ Texas Sch Publ Hlth, Austin, TX USA. [Thompson, Deborah I.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Lee, Rebecca E.] Arizona State Univ, Phoenix, AZ USA. RP Mama, SK (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Hlth Dispar Res, 1400 Pressler St,Unit 1440, Houston, TX 77030 USA. EM skmama@mdanderson.org OI McCurdy, Sheryl/0000-0003-0526-1608 FU National Institutes of Health's National Institute of Nursing Research [F31 NR013349]; National Cancer Institute [R01 CA109403] FX The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the National Institutes of Health's National Institute of Nursing Research (F31 NR013349) and the National Cancer Institute (R01 CA109403). NR 34 TC 2 Z9 3 U1 3 U2 13 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1090-1981 EI 1552-6127 J9 HEALTH EDUC BEHAV JI Health Educ. Behav. PD JUN PY 2015 VL 42 IS 3 BP 321 EP 328 DI 10.1177/1090198114557128 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CI8GE UT WOS:000355007600005 PM 25504569 ER PT J AU Williams, PA Cates, SC Blitstein, JL Hersey, JC Kosa, KM Long, VA Singh, A Berman, D AF Williams, Pamela A. Cates, Sheryl C. Blitstein, Jonathan L. Hersey, James C. Kosa, Katherine M. Long, Valerie A. Singh, Anita Berman, Danielle TI Evaluating the Impact of Six Supplemental Nutrition Assistance Program Education Interventions on Children's At-Home Diets SO HEALTH EDUCATION & BEHAVIOR LA English DT Article DE child; milk; nutrition education; school; SNAP-Ed; vegetables ID FAT-REDUCED DIET; PRESCHOOL-CHILDREN; VEGETABLE INTAKE; CONTROLLED-TRIAL; US CHILDREN; FRUIT; RISK; CALCIUM; CONSUMPTION; HEALTH AB Background. Nutrition education in the Supplemental Nutrition Assistance Program Education (SNAP-Ed) is designed to promote healthy eating behaviors in a low-income target population. Purpose. To evaluate the effectiveness of six SNAP-Ed interventions delivered in child care centers or elementary school settings in increasing participating children's at-home fruit and vegetable (F/V) consumption by 0.3 cups per day and use of fat-free or low-fat milk instead of whole or reduced-fat milk during the prior week. Method. Clustered randomized or quasi-experimental clustered trials took place in child care centers or elementary schools between 2010 and 2012. Parents of children at intervention and control sites completed baseline and follow-up surveys about their child's at home F/V consumption and other dietary behaviors. Results. One of the six interventions was successful in meeting the objective of increasing children's F/V consumption by 0.3 cups per day. For three of the six interventions, there was a small but statistically significant increase in F/V consumption and/or use of low-fat or fat-free milk. Conclusion. Although not all interventions were effective, these findings suggest that it is possible for some SNAP-Ed interventions to improve dietary habits among low-income children among some families. The effective interventions appear to have benefited from implementation experience and sustained efforts at intervention refinement and improvement. C1 [Williams, Pamela A.; Cates, Sheryl C.; Blitstein, Jonathan L.; Hersey, James C.; Kosa, Katherine M.] RTI Int, Res Triangle Pk, NC 27709 USA. [Long, Valerie A.] Altarum Inst, Portland, ME USA. [Singh, Anita; Berman, Danielle] USDA, Alexandria, VA USA. RP Kosa, KM (reprint author), RTI Int, 3040 Cornwallis Rd,POB 12194, Res Triangle Pk, NC 27709 USA. EM kkosa@rti.org FU Food and Nutrition Service, U.S. Department of Agriculture [AG-3198-D-09-0096] FX The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The work was funded under Contract Number AG-3198-D-09-0096 with the Food and Nutrition Service, U.S. Department of Agriculture. NR 41 TC 2 Z9 2 U1 1 U2 16 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1090-1981 EI 1552-6127 J9 HEALTH EDUC BEHAV JI Health Educ. Behav. PD JUN PY 2015 VL 42 IS 3 BP 329 EP 338 DI 10.1177/1090198114558589 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CI8GE UT WOS:000355007600006 PM 25512074 ER PT J AU Arndt, C Powell, JM Aguerre, MJ Crump, PM Wattiaux, MA AF Arndt, C. Powell, J. M. Aguerre, M. J. Crump, P. M. Wattiaux, M. A. TI Feed conversion efficiency in dairy cows: Repeatability, variation in digestion and metabolism of energy and nitrogen, and ruminal methanogens SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE feed efficiency; energy metabolism; nitrogen utilization; manure; dairy cattle ID CORN-SILAGE; ENTERIC METHANE; MILK-PRODUCTION; PROTEIN-CONCENTRATION; CARBON-DIOXIDE; ALFALFA SILAGE; BEEF-CATTLE; N EXCRETION; BALANCE; PERFORMANCE AB The objective was to study repeatability and sources of variation in feed conversion efficiency [FCE, milk kg/kg dry matter intake (DMI)] of lactating cows in mid to late lactation. Trials 1 and 2 used 16 cows (106 to 368 d in milk) grouped in 8 pairs of 1 high- and 1 low-FCE cow less than 16 d in milk apart. Trial 1 determined the repeatability of FCE during a 12-wk period. Trial 2 quantified the digestive and metabolic partitioning of energy and N with a 3-d total fecal and urine collection and measurement of CH4 and CO2 emission. Trial 3 studied selected ruminal methanogens in 2 pairs of cows fitted with rumen cannulas. Cows received a single diet including 28% corn silage, 27% alfalfa silage, 17% crude protein, and 28% neutral detergent fiber (dry matter basis). In trial 1, mean FCE remained repeatedly different and averaged 1.83 and 1.03 for high- and low-FCE cows, respectively. In trial 2, high- FCE cows consumed 21% more DMI, produced 98% more fat- and protein-corrected milk, excreted 42% less manure per kilogram of fat-and protein-corrected milk, but emitted the same daily amount of CH4 and CO2 compared with low-FCE cows. Percentage of gross energy intake lost in feces was higher (28.6 vs. 25.9%), but urinary (2.76 vs. 3.40%) and CH4 (5.23 vs. 6.99%) losses were lower in high-than low-FCE cows. Furthermore, high-FCE cows partitioned 15% more of gross energy intake toward net energy for maintenance, body gain, and lactation (37.5 vs. 32.6%) than low-FCE cows. Lower metabolic efficiency and greater heat loss in low-FCE cows might have been associated in part with greater energy demand for immune function related to subclinical mastitis, as somatic cell count was 3.8 fold greater in low-than high-FCE cows. As a percentage of N intake, high-FCE cows tended to have greater fecal N (32.4 vs. 30.3%) and had lower urinary N (32.2 vs. 41.7%) and greater milk N (30.3 vs. 19.1%) than low-FCE cows. In trial 3, Methanobrevibacter spp. strain AbM4 was less prevalent in ruminal content of high-FCE cows, which emitted less CH4 per unit of DMI and per unit of neutral detergent fiber digested than low-FCE cows. Thus lower digestive efficiency was more than compensated by greater metabolic efficiencies in high-compared with low-FCE cows. There was not a single factor, but rather a series of mechanisms involved in the observed differences in efficiency of energy utilization of the lactating cows in this study. C1 [Arndt, C.; Aguerre, M. J.; Wattiaux, M. A.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Powell, J. M.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Crump, P. M.] Univ Wisconsin, Dept Comp & Biometry, Madison, WI 53706 USA. RP Wattiaux, MA (reprint author), Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. EM wattiaux@wisc.edu FU USDA-ARS [58-3655-7-627]; USDA-Hatch Multi-State Research Formula fund [NE-1044, WIS01547]; USDA National Institute of Food and Agriculture [2013-68002-20525] FX This research was supported in part by the USDA-ARS specific cooperative agreement #58-3655-7-627, by USDA-Hatch Multi-State Research Formula fund NE-1044 award number WIS01547, and by USDA National Institute of Food and Agriculture grant #2013-68002-20525 climate change mitigation and adaptation in dairy production systems of the great lakes region. The authors thank Robbin Smith (US Dairy Forage Research Center, Madison, WI) for her assistance in chamber maintenance and conduct of these experiments; Nancy Betzold and her barn crew for feeding and animal care at the US Dairy Forage Center Research Farm (Prairie du Sac, WI); Mary Beth Hall (US Dairy Forage Research Center) for providing the rumen boluses, and Rebecca Holloway and other University of Wisconsin-Madison students for helping during sample collection and processing. NR 53 TC 5 Z9 6 U1 8 U2 59 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2015 VL 98 IS 6 BP 3938 EP 3950 DI 10.3168/jds.2014-8449 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CI3GB UT WOS:000354634600037 PM 25841962 ER PT J AU Hall, MB Nennich, TD Doane, PH Brink, GE AF Hall, M. B. Nennich, T. D. Doane, P. H. Brink, G. E. TI Total volatile fatty acid concentrations are unreliable estimators of treatment effects on ruminal fermentation in vivo SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE volatile fatty acid concentration; ruminal fermentation; rumen digesta; regression analysis ID LACTATING DAIRY-COWS; RUMEN LIQUID VOLUME; PROTEIN DEGRADABILITY; DILUTION RATE; SHEEP; ABSORPTION; WATER; BLOOD; DIETS; PH AB Volatile fatty acid concentrations ([VFA], mM) have long been used to assess the effect of dietary treatments on ruminal fermentation in vivo. However, discrepancies in statistical results between [VFA] and VFA pool size (VFAmol) possibly related to ruminal digesta liquid amount (LIQ, kg) indicate potential issues with the use of [VFA]. We investigated relationships among [VFA], VFAmol, and LIQ measured 2 h postfeeding using individual lactating cow data (n = 175) from 7 separate feeding studies. Regression analyses were performed using mixed models with "study" as a discrete random variable. The mean across studies and average range of values within studies, respectively, were 151 and 75 for [VFA], 11.2 and 9.8 for VFAmol, 73.3 and 41.0 for LIQ, and 289 and 83 mmol/kg for rumen fluid osmolality. Liquid amount changed with VFAmol (3.76 VFAmol + 31.2; average within-study R-2 = 0.69), but the relationship was weak between [VFA] and LIQ (0.524 LIQ + 112.8; average within-study R-2 = 0.12). The relationship between LIQ and VFAmol was likely a function of the osmotic gradient between rumen liquid and blood. The VFA are a major ruminal solute; VFAmol amounts can affect water flux in the rumen as similar tonicities of rumen fluid and blood are maintained. This also has a damping effect on ruminal solute concentration, creating the weak relationship between [VFA] and LIQ. Within studies, similar [VFA] were found in LIQ differing by 30 kg or more. The difference between minimum and maximum LIQ within cow within study was 12.7 kg (standard deviation = 7.1), so inclusion of "cow" in analyses did not correct for the variation in LIQ. To allow valid comparisons of experimental treatments, responses must be on an equivalent basis; concentrations in different LIQ are not on an equivalent basis and so are not valid to use for comparing treatment effects. The [VFA] changed with VFAmol (5.80 VFAmol + 86.3; average within-study R-2 = 0.56). However, the ratio of [VFA] to VFAmol ranged from 9.0 to 24.1 as a function of 1,000/LIQ; this reflects the inherent calculated relationship among the variables. The varying relationship of [VFA] to VFAmol further indicates that [VFA] is not an appropriate measure to evaluate the progress or effect of treatments on ruminal fermentation. Predictions of LIQ and VFAmol using cow and ruminal measures were insufficiently precise to be used in research. Previously drawn conclusions based on [VFA] need to be reevaluated, and alternate evaluations for in vivo ruminal fermentation are needed. C1 [Hall, M. B.; Brink, G. E.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Nennich, T. D.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Doane, P. H.] ADM Res, Decatur, IL 62526 USA. RP Hall, MB (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM marybeth.hall@ars.usda.gov NR 27 TC 6 Z9 6 U1 1 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2015 VL 98 IS 6 BP 3988 EP 3999 DI 10.3168/jds.2014-8854 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CI3GB UT WOS:000354634600041 PM 25828661 ER PT J AU Hain, CR Crow, WT Anderson, MC Yilmaz, MT AF Hain, Christopher R. Crow, Wade T. Anderson, Martha C. Yilmaz, M. Tugrul TI Diagnosing Neglected Soil Moisture Source-Sink Processes via a Thermal Infrared-Based Two-Source Energy Balance Model SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID LAND-SURFACE MODEL; PART I; EVAPOTRANSPIRATION ALGORITHM; WATER-TABLE; MODIS DATA; LEAF-AREA; FLUXES; SYSTEM; SCALES; IMPLEMENTATION AB In recent years, increased attention has been paid to the role of previously neglected water source (e.g., irrigation, direct groundwater extraction, and inland water bodies) and sink (e.g., tile drainage) processes on the surface energy balance. However, efforts to parameterize these processes within land surface models (LSMs) have generally been hampered by a lack of appropriate observational tools for directly observing the impact(s) of such processes on surface energy fluxes. One potential strategy for quantifying these impacts are direct comparisons between bottom-up surface energy flux predictions from a one-dimensional, free-drainage LSM with top-down energy flux estimates derived via thermal infrared remote sensing. The neglect of water source (and/or sink) processes in the bottom-up LSM can be potentially diagnosed through the presence of systematic energy flux biases relative to the top-down remote sensing retrieval. Based on this concept, the authors introduce the Atmosphere-Land Exchange Inverse (ALEXI) Source-Sink for Evapotranspiration (ASSET) index derived from comparisons between ALEXI remote sensing latent heat flux retrievals and comparable estimates obtained from the Noah LSM, version 3.2. Comparisons between ASSET index values and known spatial variations of groundwater depth, irrigation extent, inland water bodies, and tile drainage density within the contiguous United States verify the ability of ASSET to identify regions where neglected soil water source-sink processes may be impacting modeled surface energy fluxes. Consequently, ASSET appears to provide valuable information for ongoing efforts to improve the parameterization of new water source-sink processes within modern LSMs. C1 [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20740 USA. [Crow, Wade T.; Anderson, Martha C.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Yilmaz, M. Tugrul] Middle E Tech Univ, Div Water Resources, Dept Civil Engn, TR-06531 Ankara, Turkey. RP Hain, CR (reprint author), Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, 5825 Univ Res Ct,Suite 4001, College Pk, MD 20740 USA. EM chris.hain@noaa.gov RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU NASA Applied Sciences Water Resources Program [NNX12AK90G]; NOAA Climate Program Office [NA11OAR4310139] FX This work was supported by NASA Applied Sciences Water Resources Program Award NNX12AK90G, "Development of a Multi-Scale Remote-Sensing Based Framework for Mapping Drought over North America," and NOAA Climate Program Office Award NA11OAR4310139, "Dual Assimilation of Microwave and Thermal-Infrared Satellite Observations of Soil Moisture into NLDAS for Improved Drought Monitoring." We wish to thank the anonymous reviewers for helping to significantly improve the quality of the manuscript. NR 60 TC 8 Z9 8 U1 1 U2 11 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUN PY 2015 VL 16 IS 3 BP 1070 EP 1086 DI 10.1175/JHM-D-14-0017.1 PG 17 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CI9YI UT WOS:000355126500007 ER PT J AU Coopersmith, EJ Cosh, MH Petersen, WA Prueger, J Niemeier, JJ AF Coopersmith, Evan J. Cosh, Michael H. Petersen, Walt A. Prueger, John Niemeier, James J. TI Soil Moisture Model Calibration and Validation: An ARS Watershed on the South Fork Iowa River SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID DATA ASSIMILATION SYSTEM; FLOW DURATION CURVES; REGIONAL PATTERNS; PHYSICAL CONTROLS; REGIME CURVE; PRODUCTS; SMEX02 AB Soil moisture monitoring with in situ technology is a time-consuming and costly endeavor for which a method of increasing the resolution of spatial estimates across in situ networks is necessary. Using a simple hydrologic model, the estimation capacity of an in situ watershed network can be increased beyond the station distribution by using available precipitation, soil, and topographic information. A study site was selected on the Iowa River, characterized by homogeneous soil and topographic features, reducing the variables to precipitation only. Using 10-km precipitation estimates from the North American Land Data Assimilation System (NLDAS) for 2013, high-resolution estimates of surface soil moisture were generated in coordination with an in situ network, which was deployed as part of the Iowa Flood Studies (IFloodS). A simple, bucket model for soil moisture at each in situ sensor was calibrated using four precipitation products and subsequently validated at both the sensor for which it was calibrated and other proximal sensors, the latter after a bias correction step. Average RMSE values of 0.031 and 0.045 m(3) m(-3) were obtained for models validated at the sensor for which they were calibrated and at other nearby sensors, respectively. C1 [Coopersmith, Evan J.; Cosh, Michael H.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Petersen, Walt A.] NASA Wallops Space Flight Facil, Wallops, VA USA. [Prueger, John] ARS, Natl Lab Agr & Environm, USDA, Ames, IA USA. [Niemeier, James J.] Univ Iowa, IIHR Hydrosci & Engn, Iowa City, IA USA. RP Coopersmith, EJ (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, 10300 Baltimore Ave,Bldg 007, Beltsville, MD 20705 USA. EM evan.coopersmith@ars.usda.gov RI Measurement, Global/C-4698-2015 FU NASA Terrestrial Hydrology Program [NNH10ZDA001N-THP] FX USDA is an equal opportunity provider and employer. This work is supported by the NASA Terrestrial Hydrology Program (NNH10ZDA001N-THP). NR 24 TC 7 Z9 7 U1 0 U2 11 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUN PY 2015 VL 16 IS 3 BP 1087 EP 1101 DI 10.1175/JHM-D-14-0145.1 PG 15 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CI9YI UT WOS:000355126500008 ER PT J AU Han, EJ Crow, WT Hain, CR Anderson, MC AF Han, Eunjin Crow, Wade T. Hain, Christopher R. Anderson, Martha C. TI On the Use of a Water Balance to Evaluate Interannual Terrestrial ET Variability SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID MODELING SYSTEM; EVAPOTRANSPIRATION; IMPLEMENTATION; EVAPORATION; SCALES AB Accurately measuring interannual variability in terrestrial evapotranspiration ET is a major challenge for efforts to detect trends in the terrestrial hydrologic cycle. Based on comparisons with annual values of terrestrial evapotranspiration [GRAPHICS] derived from a terrestrial water balance analysis, past research has cast doubt on the ability of existing products to accurately capture [GRAPHICS] variability. Using a variety of [GRAPHICS] estimates, this analysis reexamines this conclusion and finds that estimates of [GRAPHICS] variations obtained from a land surface model are more strongly correlated with [GRAPHICS] independently acquired from thermal infrared remote sensing than [GRAPHICS] derived from water balance considerations. This tendency is attributed to significant interannual variations in terrestrial water storage neglected by the water balance approach. Overall, results demonstrate the need to reassess perceptions concerning the skill of [GRAPHICS] estimates derived from land surface models and show the value of accurate remotely sensed ET products for the validation of interannual ET. C1 [Han, Eunjin] Columbia Univ, Int Res Inst Climate & Soc, Earth Inst, Palisades, NY 10964 USA. [Crow, Wade T.; Anderson, Martha C.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Han, EJ (reprint author), Columbia Univ, Int Res Inst Climate & Soc, Earth Inst, 61 Route 9W,Monell Bldg, Palisades, NY 10964 USA. EM eunjin@iri.columbia.edu RI Han, Eunjin /G-4121-2013; Anderson, Martha/C-1720-2015 OI Han, Eunjin /0000-0001-6208-7410; Anderson, Martha/0000-0003-0748-5525 FU Water Resources Group within the NASA Applied Sciences Program FX Research was funded by a grant to Wade T. Crow (PI) from the Water Resources Group within the NASA Applied Sciences Program. NR 19 TC 2 Z9 2 U1 3 U2 9 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUN PY 2015 VL 16 IS 3 BP 1102 EP 1108 DI 10.1175/JHM-D-14-0175.1 PG 7 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CI9YI UT WOS:000355126500009 ER PT J AU Midtbo, LK Borkowska, AG Bernhard, A Ronnevik, AK Lock, EJ Fitzgerald, ML Torstensen, BE Liaset, B Brattelid, T Pedersen, TL Newman, JW Kristiansen, K Madsen, L AF Midtbo, Lisa Kolden Borkowska, Alison G. Bernhard, Annette Ronnevik, Alexander Krokedal Lock, Erik-Jan Fitzgerald, Michael L. Torstensen, Bente E. Liaset, Bjorn Brattelid, Trond Pedersen, Theresa L. Newman, John W. Kristiansen, Karsten Madsen, Lise TI Intake of farmed Atlantic salmon fed soybean oil increases hepatic levels of arachidonic acid-derived oxylipins and ceramides in mice SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE EPA; DHA; Obesity; Diabetes; Aquaculture; Lipidomics ID FATTY LIVER-DISEASE; INDUCED INSULIN-RESISTANCE; DOCOSAHEXAENOIC ACID; FISH-OIL; SALAR L.; EICOSAPENTAENOIC ACID; METABOLIC SYNDROME; LIPID-METABOLISM; SKELETAL-MUSCLE; DIET AB Introduction of vegetable ingredients in fish feed has affected the fatty acid composition in farmed Atlantic salmon (Salmo solar L). Here we investigated how changes in fish feed affected the metabolism of mice fed diets containing fillets from such farmed salmon. We demonstrate that replacement of fish oil with rapeseed oil or soybean oil in fish feed had distinct spillover effects in mice fed western diets containing the salmon. A reduced ratio of n-3/n-6 polyunsaturated fatty acids in the fish feed, reflected in the salmon, and hence also in the mice diets, led to a selectively increased abundance of arachidonic acid in the phospholipid pool in the livers of the mice. This was accompanied by increased levels of hepatic ceramides and arachidonic acid-derived pro-inflammatory mediators and a reduced abundance of oxylipins derived from eicosapentaenoic acid and docosahexaenoic acid. These changes were associated with increased whole body insulin resistance and hepatic steatosis. Our data suggest that an increased ratio between n-6 and n-3-derived oxylipins may underlie the observed marked metabolic differences between mice fed the different types of farmed salmon. These findings underpin the need for carefully considering the type of oil used for feed production in relation to salmon farming. (C) 2015 The Authors. Published by Elsevier Inc. C1 [Midtbo, Lisa Kolden; Borkowska, Alison G.; Bernhard, Annette; Ronnevik, Alexander Krokedal; Kristiansen, Karsten; Madsen, Lise] Univ Copenhagen, Dept Biol, DK-2200 Copenhagen N, Denmark. [Midtbo, Lisa Kolden; Bernhard, Annette; Ronnevik, Alexander Krokedal; Lock, Erik-Jan; Torstensen, Bente E.; Liaset, Bjorn; Brattelid, Trond; Madsen, Lise] Natl Inst Nutr & Seafood Res, Bergen, Norway. [Borkowska, Alison G.; Fitzgerald, Michael L.] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Boston, MA 02114 USA. [Borkowska, Alison G.; Pedersen, Theresa L.; Newman, John W.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Albany, CA USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, West Coast Metabol Ctr, Davis, CA 95616 USA. RP Kristiansen, K (reprint author), Univ Copenhagen, Dept Biol, Ole Maaloes Vej 5, DK-2200 Copenhagen N, Denmark. EM kk@bio.ku.dk; lise.madsen@nifes.no RI Madsen, Lise/C-6246-2012; Kristiansen, Karsten/J-5148-2014 OI Madsen, Lise/0000-0003-4468-1947; Kristiansen, Karsten/0000-0002-6024-0917 FU Research Council of Norway [199626]; West Coast Metabolomics Center [NIH 1 U24 DK097154]; intramural USDA-ARS Project [5306-51530-019-00D] FX This study was funded by the Research Council of Norway, project 199626. Work was funded in part by the following: the West Coast Metabolomics Center (NIH 1 U24 DK097154); intramural USDA-ARS Project 5306-51530-019-00D (JWN). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The USDA is an equal opportunity provider and employer. NR 65 TC 5 Z9 5 U1 1 U2 17 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD JUN PY 2015 VL 26 IS 6 BP 585 EP 595 DI 10.1016/j.jnutbio.2014.12.005 PG 11 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA CI6ZM UT WOS:000354912700002 PM 25776459 ER PT J AU Dunn, RO Ngo, HL Haas, MJ AF Dunn, Robert O. Ngo, Helen L. Haas, Michael J. TI Branched-Chain Fatty Acid Methyl Esters as Cold Flow Improvers for Biodiesel SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Branched-chain fatty acid methyl esters; Cloud point; Fatty acid methyl esters; Kinematic viscosity; Pour point ID LOW-TEMPERATURE PROPERTIES; ALTERNATIVE DIESEL FUELS; FILTER PLUGGING POINT; VEGETABLE-OIL; CLOUD POINT; CRYSTALLIZATION BEHAVIOR; OXIDATIVE STABILITY; KINEMATIC VISCOSITY; PHYSICAL-PROPERTIES; ENGINE PERFORMANCE AB Biodiesel is an alternative diesel fuel derived mainly from the transesterification of plant oils with methanol or ethanol. This fuel is generally made from commodity oils such as canola, palm or soybean and has a number of properties that make it compatible in compression-ignition engines. Despite its many advantages, biodiesel has poor cold flow properties that may impact its deployment during cooler months in moderate temperature climates. This work is a study on the use of skeletally branched-chain-fatty acid methyl esters (BC-FAME) as additives and diluents to decrease the cloud point (CP) and pour point (PP) of biodiesel. Two BC-FAME, methyl iso-oleate and methyl iso-stearate isomers (Me iso-C-18:1 and Me iso-C-18:0), were tested in mixtures with fatty acid methyl esters (FAME) of canola, palm and soybean oil (CaME, PME and SME). Results showed that mixing linear FAME with up to 2 mass% BC-FAME did not greatly affect CP, PP or kinematic viscosity (nu) relative to the unmixed biodiesel fuels. In contrast, higher concentrations of BC-FAME, namely between 17 and 39 mass%, significantly improved CP and PP without raising nu in excess of limits in the biodiesel fuel standard specification ASTM D 6751. Furthermore, it is shown that biodiesel/Me iso-C-18:0 mixtures matched or exceeded the performance of biodiesel/Me iso-C-18:1 mixtures in terms of decreasing CP and PP under certain conditions. This was taken as evidence that additives or diluents with chemical structures based on long-chain saturated chains may be more effective at reducing the cold flow properties of mixtures with biodiesel than structures based on long-chain unsaturated chains. C1 [Dunn, Robert O.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Ngo, Helen L.; Haas, Michael J.] USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Dunn, RO (reprint author), USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Robert.Dunn@ars.usda.gov NR 71 TC 5 Z9 5 U1 5 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2015 VL 92 IS 6 BP 853 EP 869 DI 10.1007/s11746-015-2643-2 PG 17 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CI5UW UT WOS:000354826300009 ER PT J AU Hojilla-Evangelista, MP Selling, GW Berhow, MA Evangelista, RL AF Hojilla-Evangelista, Mila P. Selling, Gordon W. Berhow, Mark A. Evangelista, Roque L. TI Extraction, Composition and Functional Properties of Pennycress (Thlaspi arvense L.) Press Cake Protein SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Pennycress; Pennycress proteins; Press cake; Protein extraction; Protein functionality ID CHEMICAL-COMPOSITION; CANOLA PROTEINS; SEED; OIL; ULTRAFILTRATION; SOLUBILITY; PROFILE AB This study compared two methods for extracting the protein in pennycress (Thlaspi arvense L.) press cake and determined the composition and functional properties of the protein products. Proteins in pennycress press cake were extracted by using the conventional alkali-solubilization-acid-precipitation (AP) method or saline-based (SE) procedure (0.1 M NaCl at 50 A degrees C). The extraction method has a major influence on the purity and functional properties of press cake protein products. AP had a lower protein yield (23 %) but much higher purity (90 % crude protein) compared with SE (45 % yield, 67 % crude protein). AP protein isolate had high foam capacity (120 ml), high foam stability (96 % foam volume retention) and high emulsion stability (24-35 min), and it was resistant to heat denaturation (3 % loss of solubility at pH 2 and pH 10). On the other hand, SE protein concentrate showed remarkably high solubility (> 76 %) between pH 2 and 10 and exceptional emulsifying activity (226-412 m(2)/g protein), but was more susceptible to heat denaturation at pH 7 and pH 10 (65-78 % loss of solubility). These results strongly demonstrate that higher purity pennycress press cake protein can be produced by either saline extraction or acid precipitation and have functional properties that are desirable for non-food uses. C1 [Hojilla-Evangelista, Mila P.; Selling, Gordon W.] ARS, Plant Polymer Res Unit, NCAUR, USDA, Peoria, IL 61604 USA. [Berhow, Mark A.] ARS, Funct Foods Res Unit, NCAUR, USDA, Peoria, IL 61604 USA. [Evangelista, Roque L.] ARS, Biooils Res Unit, NCAUR, USDA, Peoria, IL 61604 USA. RP Hojilla-Evangelista, MP (reprint author), ARS, Plant Polymer Res Unit, NCAUR, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Mila.HojillaEvangelista@ars.usda.gov NR 37 TC 2 Z9 2 U1 0 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2015 VL 92 IS 6 BP 905 EP 914 DI 10.1007/s11746-015-2653-0 PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CI5UW UT WOS:000354826300013 ER PT J AU Nelson, MI Stratton, J Killian, ML Janas-Martindale, A Vincent, AL AF Nelson, Martha I. Stratton, Jered Killian, Mary Lea Janas-Martindale, Alicia Vincent, Amy L. TI Continual Reintroduction of Human Pandemic H1N1 Influenza A Viruses into Swine in the United States, 2009 to 2014 SO JOURNAL OF VIROLOGY LA English DT Article ID PIGS; TRANSMISSION; EVOLUTION; FARM; REASSORTMENT; MODELS AB The diversity of influenza A viruses in swine (swIAVs) presents an important pandemic threat. Knowledge of the human-swine interface is particularly important for understanding how viruses with pandemic potential evolve in swine hosts. Through phylogenetic analysis of contemporary swIAVs in the United States, we demonstrate that human-to-swine transmission of pandemic H1N1 (pH1N1) viruses has occurred continuously in the years following the 2009 H1N1 pandemic and has been an important contributor to the genetic diversity of U.S. swIAVs. Although pandemic H1 and N1 segments had been largely removed from the U.S. swine population by 2013 via reassortment with other swIAVs, these antigens reemerged following multiple human-to-swine transmission events during the 2013-2014 seasonal epidemic. These findings indicate that the six internal gene segments from pH1N1 viruses are likely to be sustained long term in the U.S. swine population, with periodic reemergence of pandemic hemagglutinin (HA) and neuraminidase (NA) segments in association with seasonal pH1N1 epidemics in humans. Vaccinating U.S. swine workers may reduce infection of both humans and swine and in turn limit the role of humans as sources of influenza virus diversity in pigs. IMPORTANCE Swine are important hosts in the evolution of influenza A viruses with pandemic potential. Here, we analyze influenza virus sequence data generated by the U.S. Department of Agriculture's national surveillance system to identify the central role of humans in the reemergence of pandemic H1N1 (pH1N1) influenza viruses in U.S. swine herds in 2014. These findings emphasize the important role of humans as continuous sources of influenza virus diversity in swine and indicate that influenza viruses with pandemic HA and NA segments are likely to continue to reemerge in U.S. swine in association with seasonal pH1N1 epidemics in humans. C1 [Nelson, Martha I.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Stratton, Jered; Vincent, Amy L.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Killian, Mary Lea; Janas-Martindale, Alicia] APHIS, Diagnost Virol Lab, Natl Vet Serv Labs, Sci Technol & Anal Serv,Vet Serv,USDA, Ames, IA USA. RP Nelson, MI (reprint author), NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM nelsonma@mail.nih.gov FU Multinational Influenza Seasonal Mortality Study (MISMS); Office of Global Affairs at the Department of Health and Human Services; USDA-ARS; USDA-APHIS FX This work was supported in part by the Multinational Influenza Seasonal Mortality Study (MISMS), an ongoing international collaborative effort to understand influenza epidemiology and evolution, led by the Fogarty International Center, National Institutes of Health, with funding from the Office of Global Affairs at the Department of Health and Human Services (M.I.N.). Funding was provided by USDA-ARS (J.S. and A.L.V.) and by USDA-APHIS (A.J.-M. and M.L.K.). NR 36 TC 19 Z9 20 U1 0 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD JUN PY 2015 VL 89 IS 12 BP 6218 EP 6226 DI 10.1128/JVI.00459-15 PG 9 WC Virology SC Virology GA CI3OU UT WOS:000354657900007 PM 25833052 ER PT J AU Lee, DH Torchetti, MK Winker, K Ip, HS Song, CS Swayne, DE AF Lee, Dong-Hun Torchetti, Mia Kim Winker, Kevin Ip, Hon S. Song, Chang-Seon Swayne, David E. TI Intercontinental Spread of Asian-Origin H5N8 to North America through Beringia by Migratory Birds SO JOURNAL OF VIROLOGY LA English DT Article ID AVIAN INFLUENZA; SOUTH-KOREA; WILD BIRDS; VIRUS; PATTERNS; FLYWAY; ALASKA AB Phylogenetic network analysis and understanding of waterfowl migration patterns suggest that the Eurasian H5N8 clade 2.3.4.4 avian influenza virus emerged in late 2013 in China, spread in early 2014 to South Korea and Japan, and reached Siberia and Beringia by summer 2014 via migratory birds. Three genetically distinct subgroups emerged and subsequently spread along different flyways during fall 2014 into Europe, North America, and East Asia, respectively. All three subgroups reappeared in Japan, a wintering site for waterfowl from Eurasia and parts of North America. C1 [Lee, Dong-Hun; Swayne, David E.] ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr Agr,USDA, Athens, GA 30605 USA. [Torchetti, Mia Kim] USDA, Natl Vet Serv Labs, Sci Technol & Anal Serv, Vet Serv,Anim & Plant Hlth Inspect Serv, Ames, IA 50010 USA. [Winker, Kevin] Univ Alaska Fairbanks, Univ Alaska Museum, Fairbanks, AK USA. [Ip, Hon S.] US Geol Survey, Natl Wildlife Hlth Ctr, US Dept Interior, Madison, WI USA. [Song, Chang-Seon] Konkuk Univ, Coll Vet Med, Seoul, South Korea. RP Swayne, DE (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr Agr,USDA, Athens, GA 30605 USA. EM David.Swayne@ars.usda.gov RI Winker, Kevin/M-2042-2014; OI Winker, Kevin/0000-0002-8985-8104; Ip, Hon/0000-0003-4844-7533 NR 20 TC 62 Z9 65 U1 4 U2 23 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD JUN PY 2015 VL 89 IS 12 BP 6521 EP 6524 DI 10.1128/JVI.00728-15 PG 4 WC Virology SC Virology GA CI3OU UT WOS:000354657900034 PM 25855748 ER PT J AU Boyington, J Joseph, L Fielding, R Pate, R AF Boyington, Josephine Joseph, Lyndon Fielding, Roger Pate, Russell TI Sedentary Behavior Research Priorities-NHLBI/NIA Sedentary Behavior Workshop Summary SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article ID TYPE-2 DIABETES-MELLITUS; PHYSICAL-ACTIVITY; CARDIOVASCULAR-DISEASE; TIME SPENT; US ADULTS; RISK; MORTALITY; MEN; OBESITY; WOMEN C1 [Boyington, Josephine] NHLBI, NIH, Bethesda, MD 20892 USA. [Joseph, Lyndon] NIA, NIH, Bethesda, MD 20892 USA. [Fielding, Roger] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Pate, Russell] Univ S Carolina, Arnold Sch Publ Hlth, Columbia, SC 29208 USA. RP Pate, R (reprint author), Univ S Carolina, Arnold Sch Publ Hlth, Suite 212,921 Assembly St, Columbia, SC 29208 USA. EM rpate@mailbox.sc.edu FU National Heart, Lung, and Blood Institute; National Institute on Aging; Office of Disease Prevention of the National Institutes of Health; U.S. Department of Agriculture [58-19500-014]; Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679]; U.S. Department of Agriculture; National Institutes of Health; Nestec Ltd.; Regeneron Pharmaceutical; Centers for Disease Control and Prevention; Duke Endowment; FTZ Coca-Cola Service Company FX The workshop was sponsored by the National Heart, Lung, and Blood Institute, the National Institute on Aging, and the Office of Disease Prevention of the National Institutes of Health. The views expressed in this document reflect the collective ideas and opinions of the authors and do not necessarily represent the official views of the National Institutes of Health, the U.S. Department of Health and Human Services, or the other workshop participants.; Dr. Fielding's participation in this project was partially supported by the U.S. Department of Agriculture, under agreement no. 58-19500-014, and the Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679). Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Agriculture.; Dr. Fielding receives grant support from the U.S. Department of Agriculture, National Institutes of Health, Nestec Ltd., and Regeneron Pharmaceutical; is a consultant to Regeneron Pharmaceuticals and Nestec, Ltd; serves on the Scientific Advisory Boards of Cytokinetics, Inc., Pronutria, Inc., Amonnett, Inc., and Inside Tracker, Inc,; and owns stock or options in Pronutria, Amonnett, Inc., Inside Tracker, and Myosyntax.; Dr. Pate receives grant support from the National Institutes of Health, Centers for Disease Control and Prevention, Duke Endowment, and FTZ Coca-Cola Service Company. He serves on the Scientific Advisory Board of Curves, Inc. NR 17 TC 4 Z9 4 U1 0 U2 8 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0195-9131 EI 1530-0315 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD JUN PY 2015 VL 47 IS 6 BP 1291 EP 1294 DI 10.1249/MSS.0000000000000516 PG 4 WC Sport Sciences SC Sport Sciences GA CI4TC UT WOS:000354745500024 PM 25222819 ER PT J AU Jenkins, MC O'Brien, CN Santin, M Fayer, R AF Jenkins, M. C. O'Brien, C. N. Santin, M. Fayer, R. TI Changes in the levels of Cryspovirus during in vitro development of Cryptosporidium parvum SO PARASITOLOGY RESEARCH LA English DT Article DE Cryptosporidium; Cryspovirus; Cell culture ID DOUBLE-STRANDED RNAS; VIRUS-LIKE; PCR; COMBINATION; SEQUENCE; OOCYSTS; CELLS AB The purpose of this study was to develop and utilize semi-quantitative RT-PCR and PCR assays for measuring the level of Cryspovirus, the viral symbiont of Cryptosporidium parvum, during in vitro development of the protozoan. Cultures of human carcinoma cells (HCT-8) were inoculated with excysting C. parvum sporozoites, followed by harvest of cells and culture medium at 2-, 24-, 48-, and 72-h post-infection. Changes in viral RNA levels were detected by RT-PCR using primers specific for RNA encoding the 40-kDa capsid protein (CP) or RNA-dependent RNA polymerase (RdRp). Parasite or host DNA was quantified by PCR specific for C. parvum or human glyceraldehyde-3-phosphate dehydrogenase (HuGAPDH). An internal standard (competitor) was incorporated into all assays as a control for PCR inhibition. Intracellular levels of C. parvum DNA increased between 2- and 48-h post-infection, and then decreased at 72 h. Culture medium overlying these C. parvum-infected cells displayed a similar increase in CP and RdRp signal, reaching peak levels at 48 h. However, the CP and RdRp levels in cellular RNA displayed only a modest increase between 2 and 48 h, and exhibited no change (CP) or decreased (RdRp) at 72 h. These data suggest that during the first 48 h of C. parvum in vitro development, Cryspovirus is released into the media overlying cells but remains at fairly constant levels within infected cells. C1 [O'Brien, C. N.; Santin, M.; Fayer, R.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Jenkins, M. C.] BARC EAST, Beltsville, MD 20070 USA. RP Jenkins, MC (reprint author), BARC EAST, Bldg 1040, Beltsville, MD 20070 USA. EM mark.jenkins@ars.usda.gov FU USDA-ARS CRIS Project [1245-32000-089-00D] FX Funding for this research was provided under USDA-ARS CRIS Project No. 1245-32000-089-00D. NR 26 TC 0 Z9 1 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD JUN PY 2015 VL 114 IS 6 BP 2063 EP 2068 DI 10.1007/s00436-015-4390-6 PG 6 WC Parasitology SC Parasitology GA CI0CT UT WOS:000354404100003 PM 25704645 ER PT J AU Makarikov, AA Nims, TN Galbreath, KE Hoberg, EP AF Makarikov, Arseny A. Nims, Todd N. Galbreath, Kurt E. Hoberg, Eric P. TI Hymenolepis folkertsi n. sp (Eucestoda: Hymenolepididae) in the oldfield mouse Peromyscus polionotus (Wagner) (Rodentia: Cricetidae: Neotominae) from the southeastern Nearctic with comments on tapeworm faunal diversity among deer mice SO PARASITOLOGY RESEARCH LA English DT Article DE Eucestoda; Hymenolepididae; Hymenolepis folkertsi; New species; Morphology; Rodents; Peromyscus polionotus; Georgia ID CESTODA HYMENOLEPIDIDAE; PHYLOGENETIC-RELATIONSHIPS; HELMINTH-PARASITES; MAMMALS; CYCLOPHYLLIDEA; MANICULATUS; GEOMYIDAE; CLIMATE; ANOPLOCEPHALIDAE; ARVICOLINAE AB A previously unrecognized species of hymenolepidid cestode attributable to Hymenolepis is described based on specimens in Peromyscus polionotus, oldfield mouse, from Georgia near the southeastern coast of continental North America. Specimens of Hymenolepis folkertsi n. sp. differ from those attributed to most other species in the genus by having testes arranged in a triangle and a scolex with a prominent rostrum-like protrusion. The newly recognized species is further distinguished by the relative position and length of the cirrus sac, shape of seminal receptacle, and relative size of external seminal vesicle and seminal receptacle. Hymenolepidid cestodes have sporadically been reported among the highly diverse assemblage of Peromyscus which includes 56 distinct species in the Nearctic. Although the host genus has a great temporal duration and is endemic to the Nearctic, current evidence suggests that tapeworm faunal diversity reflects relatively recent assembly through bouts of host switching among other cricetid, murid, and geomyid rodents in sympatry. C1 [Makarikov, Arseny A.] Russian Acad Sci, Inst Systemat & Ecol Anim, Siberian Branch, Novosibirsk 630091, Russia. [Nims, Todd N.] Georgia Perimeter Coll, Dept Sci, Covington, GA 30014 USA. [Galbreath, Kurt E.] No Michigan Univ, Dept Biol, Marquette, MI 49855 USA. [Hoberg, Eric P.] ARS, US Natl Parasite Collect, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Makarikov, AA (reprint author), Russian Acad Sci, Inst Systemat & Ecol Anim, Siberian Branch, Frunze Str 11, Novosibirsk 630091, Russia. EM makarikov@mail.ru RI Makarikov, Arseny/I-5516-2013 OI Makarikov, Arseny/0000-0002-4389-8397 FU Russian Fund for Fundamental Research [14-04-00871-a]; National Science Foundation [DEB 0819696, 0818823]; NSF through the Beringian Coevolution Project [DEB 0196095, 0415668]; Integrated Inventory of Biomes of the Arctic (DEB-Biodiversity Discovery and Analysis) [1258010] FX We thank Dr. Jean Mariaux (Natural History Museum, Geneva, Switzerland), Dr. Patricia Pilitt (US National Parasite Collection, Beltsville, MD, USA), and Dr. Scott L. Gardner (Harold W. Manter Laboratory of Parasitology, Lincoln, NE, USA), for specimen loans and/or providing conditions and laboratory space for examination of the type and voucher specimens. We thank Dr. Oscar Pung (Georgia Southern University (GSU), Statesboro, GA, USA) for his assistance with the mouse dissections. We thank The Nature Conservancy in Georgia for allowing access to their managed lands. We are grateful for access to the Arctos database through the Museum of Southwestern Biology, University of New Mexico, and a summary of inventory for parasites in species of Peromyscus prepared by Mariel Campbell. We sincerely thank Dr. Scott L. Gardner and an anonymous reviewer for their detailed comments that improved our manuscript. Research by AAM was supported in part by the Russian Fund for Fundamental Research (Project No. 14-04-00871-a). Further support for AAM was provided by the National Science Foundation (DEB 0819696 and 0818823) through grants addressing cestode diversity coordinated by Dr. Janine Caira, University of Connecticut. This is also a contribution to understanding history and diversity of mammalian helminth faunas supported by NSF through the Beringian Coevolution Project (DEB 0196095 and 0415668) and the Integrated Inventory of Biomes of the Arctic (DEB-Biodiversity Discovery and Analysis-1258010) to J.A. Cook (University of New Mexico), EPH, and KEG. Portions of this study were approved by the Institutional Animal Care and Use Committee (IACUC) at Georgia Southern University, permitted by the Georgia Department of Natural Resources, and done in partial completion of a Master of Science in Biology at GSU by TNN. NR 67 TC 2 Z9 2 U1 3 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD JUN PY 2015 VL 114 IS 6 BP 2107 EP 2117 DI 10.1007/s00436-015-4399-x PG 11 WC Parasitology SC Parasitology GA CI0CT UT WOS:000354404100009 PM 25762188 ER PT J AU Kelly, CN Calderon, FC Acosta-Martinez, V Mikha, MM Benjamin, J Rutherford, DW Rostad, CE AF Kelly, Charlene N. Calderon, Francisco C. Acosta-Martinez, Vernica Mikha, Maysoon M. Benjamin, Joseph Rutherford, David W. Rostad, Colleen E. TI Switchgrass Biochar Effects on Plant Biomass and Microbial Dynamics in Two Soils from Different Regions SO PEDOSPHERE LA English DT Article DE correction factor; extraction efficiency; fatty acid methyl ester profile; nitrogen mineralization; soil microbial community; soil nutrient availability; wheat ID HIGHLY WEATHERED SOILS; BLACK CARBON; COMMUNITY STRUCTURE; WHEAT CROP; MANAGEMENT; NITROGEN; AMENDMENT; CHARCOAL; TROPICS; QUALITY AB Biochar amendments to soils may alter soil function and fertility in various ways, including through induced changes in the microbial community. We assessed microbial activity and community composition of two distinct clayey soil types, an Aridisol from Colorado (CO) in the U.S. Central Great Plains, and an Alfisol from Virginia (VA) in the southeastern USA following the application of switchgrass (Panicum virgatum) biochar. The switchgrass biochar was applied at four levels, 0%, 2.5%, 5%, and 10%, approximately equivalent to biochar additions of 0, 25, 50, and 100 t ha(-1), respectively, to the soils grown with wheat (Triticum aestivum) in an eight-week growth chamber experiment. We measured wheat shoot biomass and nitrogen (N) content and soil nutrient availability and N mineralization rates, and characterized the microbial fatty acid methyl ester (FAME) profiles of the soils. Net N mineralization rates decreased in both soils in proportion to an increase in biochar levels, but the effect was more marked in the VA soil, where net N mineralization decreased from -2.1 to -38.4 mg kg(-1). The 10% biochar addition increased soil pH, electrical conductivity, Mehlich- and bicarbonate-extractable phosphorus (P), and extractable potassium (K) in both soil types. The wheat shoot biomass decreased from 17.7 to 9.1 g with incremental additions of biochar in the CO soil, but no difference was noted in plants grown in the VA soil. The FAME recovery assay indicated that the switchgrass biochar addition could introduce artifacts in analysis, so the results needed to be interpreted with caution. Non-corrected total FAME concentrations indicated a decline by 45% and 34% with 10% biochar addition in the CO and VA soils, respectively, though these differences became nonsignificant when the extraction efficiency correction factor was applied. A significant decline in the fungi:bacteria ratio was still evident upon correction in the CO soil with biochar. Switchgrass biochar had the potential to cause short-term negative impacts on plant biomass and alter soil microbial community structure unless measures were taken to add supplemental N and labile carbon (C). C1 [Kelly, Charlene N.] Western Carolina Univ, Dept Geosczences & Nat Resources, Cullowhee, NC 28723 USA. [Calderon, Francisco C.; Mikha, Maysoon M.; Benjamin, Joseph] USDA ARS, Akron, OH USA. [Acosta-Martinez, Vernica] USDA ARS, Lubbock, TX 79403 USA. [Kelly, Charlene N.; Rutherford, David W.; Rostad, Colleen E.] US Geol Survey, Denver, CO 80225 USA. RP Kelly, CN (reprint author), Western Carolina Univ, Dept Geosczences & Nat Resources, 331 Stillwell Hall, Cullowhee, NC 28723 USA. EM cnkelly@email.wcu.edu NR 55 TC 4 Z9 5 U1 9 U2 71 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD JUN PY 2015 VL 25 IS 3 BP 329 EP 342 PG 14 WC Soil Science SC Agriculture GA CI5WT UT WOS:000354831200001 ER PT J AU Liu, LJ Ramsay, T Zinkgraf, M Sundell, D Street, NR Filkov, V Groover, A AF Liu, Lijun Ramsay, Trevor Zinkgraf, Matthew Sundell, David Street, Nathaniel Robert Filkov, Vladimir Groover, Andrew TI A resource for characterizing genome-wide binding and putative target genes of transcription factors expressed during secondary growth and wood formation in Populus SO PLANT JOURNAL LA English DT Article DE Populus trichocarpa; chromatin immunoprecipitation sequencing; transcription factor; cambium; secondary growth; wood formation ID CHIP-SEQ; ARABIDOPSIS; MERISTEM; BREVIPEDICELLUS; ASSOCIATION; MECHANISMS; PENNYWISE; CAMBIUM; PLANTS; MODEL AB Identifying transcription factor target genes is essential for modeling the transcriptional networks underlying developmental processes. Here we report a chromatin immunoprecipitation sequencing (ChIP-seq) resource consisting of genome-wide binding regions and associated putative target genes for four Populus homeodomain transcription factors expressed during secondary growth and wood formation. Software code (programs and scripts) for processing the Populus ChIP-seq data are provided within a publically available iPlant image, including tools for ChIP-seq data quality control and evaluation adapted from the human Encyclopedia of DNA Elements (ENCODE) project. Basic information for each transcription factor (including members of Class I KNOX, Class III HD ZIP, BEL1-like families) binding are summarized, including the number and location of binding regions, distribution of binding regions relative to gene features, associated putative target genes, and enriched functional categories of putative target genes. These ChIP-seq data have been integrated within the Populus Genome Integrative Explorer (PopGenIE) where they can be analyzed using a variety of web-based tools. We present an example analysis that shows preferential binding of transcription factor ARBORKNOX1 to the nearest neighbor genes in a pre-calculated co-expression network module, and enrichment for meristem-related genes within this module including multiple orthologs of Arabidopsis KNOTTED-like Arabidopsis 2/6. C1 [Liu, Lijun; Zinkgraf, Matthew; Groover, Andrew] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Ramsay, Trevor; Filkov, Vladimir] Univ Calif Davis, Dept Comp Sci, Davis, CA 95618 USA. [Sundell, David; Street, Nathaniel Robert] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, SE-90187 Umea, Sweden. [Groover, Andrew] Univ Calif Davis, Dept Plant Biol, Davis, CA 95618 USA. RP Groover, A (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. EM agroover@fs.fed.us RI Street, Nathaniel/B-3920-2008 OI Street, Nathaniel/0000-0001-6031-005X FU Agriculture and Food Research initiative competitive grant of the USDA National Institute of Food and Agriculture [2011-67013-30062]; NSF Postdoctoral Research Fellowship in Biology [IOS-1402064] FX We would like to thank Brian Stanton, Kathy Haiby, and Rich Shuren of Greenwood Resources for facilitating collection of cambium and developing wood/bark samples. Illumina sequencing was performed by the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley. This work was supported by the Agriculture and Food Research initiative competitive grant 2011-67013-30062 of the USDA National Institute of Food and Agriculture. MZ is supported by NSF Postdoctoral Research Fellowship in Biology Grant IOS-1402064. NR 36 TC 9 Z9 10 U1 4 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD JUN PY 2015 VL 82 IS 5 BP 887 EP 898 DI 10.1111/tpj.12850 PG 12 WC Plant Sciences SC Plant Sciences GA CJ1CO UT WOS:000355219800013 PM 25903933 ER PT J AU Mendes, RF Mendes, LM de Oliveira, JE Savastano, H Glenn, G Tonoli, GHD AF Mendes, Rafael Farinassi Mendes, Lourival Marin de Oliveira, Juliano Elvis Savastano Junior, Holmer Glenn, Gregory Denzin Tonoli, Gustavo Henrique TI Modification of eucalyptus pulp fiber using silane coupling agents with aliphatic side chains of different length SO POLYMER ENGINEERING AND SCIENCE LA English DT Article ID CEMENT-BASED COMPOSITES; CELLULOSE FIBERS; LIGNOCELLULOSIC WASTES; FUNCTIONALIZED SILANES; SURFACE-PROPERTIES; DURABILITY; CONDENSATION; PERFORMANCE; HYDROLYSIS; ADSORPTION AB The objective of this work was to evaluate the effect of three silane coupling agents with different aliphatic chain lengths on the hydrophobicity of eucalyptus pulp fiber. The three silanes coupling agents used (isobutyltrimethoxysilane, methyltrimethoxysilane, and n-octyltriethoxysilane [OTES]) were each tested at three concentrations. Scanning electron microscopy coupled with energy dispersive spectroscopy revealed that the silane coupling agents markedly increased the Si content of the treated fibers. The Si distribution was not completely homogenous but was abundant in the treated samples. The treated fibers had higher contact angles for water and lower moisture adsorption than the control. Of the coupling agents tested, OTES treated fibers had the highest moisture resistance. This was likely due to the greater chain length of the aliphatic side group coupled to OTES. The use of silane coupling agents with alkyl side chains could improve the functional properties of pulp fiber and increase its compatibility with hydrophobic polymers. POLYM. ENG. SCI., 55:1273?1280, 2015. (c) 2015 Society of Plastics Engineers C1 [Mendes, Rafael Farinassi; de Oliveira, Juliano Elvis] Univ Fed Lavras, Dept Engn, BR-37200000 Lavras, MG, Brazil. [Mendes, Lourival Marin; Denzin Tonoli, Gustavo Henrique] Univ Fed Lavras, Dept Forest Sci, BR-37200000 Lavras, MG, Brazil. [Savastano Junior, Holmer] Univ Sao Paulo, Dept Biosyst Engn, Fac Zootecnia Engn & Alimentos, BR-13635900 Sao Paulo, Brazil. [Glenn, Gregory] USDA ARS, WRRC, Bioprod Res Unit, Albany, CA 94710 USA. RP Mendes, RF (reprint author), Univ Fed Lavras, Dept Engn, BR-37200000 Lavras, MG, Brazil. EM rafaelfarinassi@gmail.com RI Savastano Junior, Holmer/B-8369-2012 OI Savastano Junior, Holmer/0000-0003-1827-1047 FU Brazilian Research Council (CNPq); Research Foundation of Minas Gerais (FAPEMIG) FX Contract grant sponsor: Brazilian Research Council (CNPq) and the Research Foundation of Minas Gerais (FAPEMIG). NR 46 TC 0 Z9 0 U1 1 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-3888 EI 1548-2634 J9 POLYM ENG SCI JI Polym. Eng. Sci. PD JUN PY 2015 VL 55 IS 6 BP 1273 EP 1280 DI 10.1002/pen.24065 PG 8 WC Engineering, Chemical; Polymer Science SC Engineering; Polymer Science GA CI4RM UT WOS:000354740200007 ER PT J AU Cainong, JC Bockus, WW Feng, YG Chen, PD Qi, LL Sehgal, SK Danilova, TV Koo, DH Friebe, B Gill, BS AF Cainong, Joey C. Bockus, William W. Feng, Yigao Chen, Peidu Qi, Lili Sehgal, Sunish K. Danilova, Tatiana V. Koo, Dal-Hoe Friebe, Bernd Gill, Bikram S. TI Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID TRITICUM-AESTIVUM L.; BREAD WHEAT; MAJOR QTL; BAC-FISH; GENOME AB This manuscript describes the transfer and molecular cytogenetic characterization of a novel source of Fusarium head blight resistance in wheat. Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe [telomorph = Gibberella zeae (Schwein. Fr.) Petch] is an important disease of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD) worldwide. Wheat has limited resistance to FHB controlled by many loci and new sources of resistance are urgently needed. The perennial grass Elymus tsukushiensis thrives in the warm and humid regions of China and Japan and is immune to FHB. Here, we report the transfer and mapping of a major gene Fhb6 from E. tsukushiensis to wheat. Fhb6 was mapped to the subterminal region in the short arm of chromosome 1E(ts)#1S of E. tsukushiensis. Chromosome engineering was used to replace corresponding homoeologous region of chromosome 1AS of wheat with the Fhb6 associated chromatin derived from 1E(ts)#1S of E. tsukushiensis. Fhb6 appears to be new locus for wheat as previous studies have not detected any FHB resistance QTL in this chromosome region. Plant progenies homozygous for Fhb6 had a disease severity rating of 7 % compared to 35 % for the null progenies. Fhb6 has been tagged with molecular markers for marker-assisted breeding and pyramiding of resistance loci for effective control of FHB. C1 [Cainong, Joey C.; Bockus, William W.; Sehgal, Sunish K.; Danilova, Tatiana V.; Koo, Dal-Hoe; Friebe, Bernd; Gill, Bikram S.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Plant Sci Ctr, Manhattan, KS 66506 USA. [Feng, Yigao; Chen, Peidu] Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China. [Qi, Lili] USDA ARS, Crop Sci Lab, Fargo, ND 58102 USA. [Sehgal, Sunish K.] S Dakota State Univ, Brookings, SD 57006 USA. RP Friebe, B (reprint author), Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Plant Sci Ctr, Manhattan, KS 66506 USA. EM friebe@ksu.edu FU U.S. Wheat and Barley Scab Initiative; Kansas Wheat Commission; Kansas Crop Improvement Association; WGRC I/UCRC NSF [1338897] FX We thank W. John Raupp and Robert R McIntosh for critical editorial review of the manuscript and Duane Wilson for technical assistance. This research was supported by grants from the U.S. Wheat and Barley Scab Initiative, the Kansas Wheat Commission, the Kansas Crop Improvement Association and WGRC I/UCRC NSF contract 1338897. This is contribution number 14-395-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, U.S.A. NR 28 TC 9 Z9 12 U1 3 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2015 VL 128 IS 6 BP 1019 EP 1027 DI 10.1007/s00122-015-2485-1 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CI3FU UT WOS:000354633800002 PM 25726000 ER PT J AU Naruoka, Y Garland-Campbell, KA Carter, AH AF Naruoka, Y. Garland-Campbell, K. A. Carter, A. H. TI Genome-wide association mapping for stripe rust (Puccinia striiformis F. sp tritici) in US Pacific Northwest winter wheat (Triticum aestivum L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID ADULT-PLANT RESISTANCE; QUANTITATIVE TRAIT LOCI; MARKER-ASSISTED SELECTION; HIGH-TEMPERATURE; SPRING WHEAT; LEAF RUST; YELLOW RUST; BREAD WHEAT; POPULATION-STRUCTURE; COMMON WHEAT AB Potential novel and known QTL for race-specific all-stage and adult plant resistance to stripe rust were identified by genome-wide association mapping in the US PNW winter wheat accessions. Stripe rust (Puccinia striiformis F. sp. tritici; also known as yellow rust) is a globally devastating disease of wheat (Triticum aestivum L.) and a major threat to wheat production in the US Pacific Northwest (PNW), therefore both adult plant and all-stage resistance have been introduced into the winter wheat breeding programs in the PNW. The goal of this study was to identify quantitative trait loci (QTL) and molecular markers for these resistances through genome-wide association (GWAS) mapping in winter wheat accessions adapted to the PNW. Stripe rust response for adult plants was evaluated in naturally occurring epidemics in a total of nine environments in Washington State, USA. Seedling response was evaluated with three races under artificial inoculation in the greenhouse. The panel was genotyped with the 9K Illumina Wheat single nucleotide polymorphism (SNP) array and additional markers linked to previously reported genes and QTL for stripe rust resistance. The population was grouped into three sub-populations. Markers linked to Yr17 and previously reported QTL for stripe rust resistance were identified on chromosomes 1B, 2A, and 2B. Potentially novel QTL associated with race-specific seedling response were identified on chromosomes 1B and 1D. Potentially novel QTL associated with adult plant response were located on chromosomes 2A, 2B, 3B, 4A, and 4B. Stripe rust was reduced when multiple alleles for resistance were present. The resistant allele frequencies were different among sub-populations in the panel. This information provides breeders with germplasm and closely linked markers for stripe rust resistance to facilitate the transfer of multiple loci for durable stripe rust resistance into wheat breeding lines and cultivars. C1 [Naruoka, Y.; Carter, A. H.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Garland-Campbell, K. A.] ARS, Wheat Genet Qual Physiol & Dis Res Unit, USDA, Pullman, WA 99164 USA. RP Naruoka, Y (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM yukiko.naruoka@wsu.edu FU Washington State University [0232]; Washington Grain Commission [5238]; National Research Initiative Competitive Grants CAP project from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This project was supported by Washington State University (Hatch project #0232), the Washington Grain Commission Project #5238, and a National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. We greatly appreciate Dr. Linda Klein for her helpful review of and edits to the manuscript. We thank Ryan Higginbotham, Gary Shelton, and Kent Evans for maintenance of the field screening locations, Kerry Balow and Anmin Wan for assistance in the greenhouse screens, and Drs. Robert E. Allan and Clarence J. Peterson for their battles with stripe rust that led to the accumulation of so many minor genes for resistance in the PNW winter wheat germplasm. NR 78 TC 10 Z9 10 U1 9 U2 65 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2015 VL 128 IS 6 BP 1083 EP 1101 DI 10.1007/s00122-015-2492-2 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CI3FU UT WOS:000354633800008 PM 25754424 ER PT J AU Singh, RJ Nelson, RL AF Singh, R. J. Nelson, R. L. TI Intersubgeneric hybridization between Glycine max and G-tomentella: production of F-1, amphidiploid, BC1, BC2, BC3, and fertile soybean plants SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID WILD PERENNIAL RELATIVES; ALIEN ADDITION LINES; INTERSPECIFIC HYBRIDIZATION; GENUS GLYCINE; PHAKOPSORA-PACHYRHIZI; WIDE HYBRIDIZATION; BREEDING-BEHAVIOR; EMBRYO CULTURE; L MERR; RESISTANCE AB This paper describes methods for unlocking genetic treasure from wild perennial Glycine species of Australia for soybean improvement. The genetic resources of the ca. 26 species of the genus Glycine subgenus Glycine have not been exploited to broaden the genetic base of soybean (Glycine max; 2n = 40). The objectives of this study were to develop methods for producing F-1, amphidiploid, BC1, BC2, BC3, and fertile soybean plants from crosses of soybean and the genus Glycine subgenus Glycine species, in order to utilize the subgenus Glycine germplasm in soybean breeding. Soybean cultivars were hybridized with six accessions of 78-chromosome G. tomentella as well as one accession each of 40-chromosome G. tomentella, G. argyrea and G. latifolia. They were chosen because they exhibit resistance to soybean rust. We were successful in producing fertile soybean from soybean cv. 'Dwight' and 78-chromosome G. tomentella accession PI 441001, while other hybrids were discontinued either at F-1 or amphidiploid stage. The F-1 seeds aborted prior to reaching maturity, so developing seeds from 19 to 21 day old pods were cultured aseptically in various media formulations. Seed maturation and multiple embryo generation media were developed. F-1 plants with shoots and roots (2n = 59) were transplanted to pots in greenhouse. Amphidiploid (2n = 118) plants were backcrossed to 'Dwight'. BC1 (2n = 79) plants were propagated through in vitro and 43 mature BC2F1 seeds were harvested. Fifteen surviving BC2F1 plants were morphologically distinct, sterile, and had chromosome numbers ranging 2n = 56-59. Chromosome numbers of the BC3F1 plants ranged 2n = 40-49. Derived fertile soybeans were first planted in the field in 2008 and are being evaluated for yield, resistance to pathogens and pests and tolerance to salt through material transfer agreement. C1 [Singh, R. J.; Nelson, R. L.] Univ Illinois, Dept Crop Sci, USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. RP Singh, RJ (reprint author), Univ Illinois, Dept Crop Sci, USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, 1101 West Peabody Dr, Urbana, IL 61801 USA. EM Ram.Singh@ars.usda.gov FU Illinois Soybean Association; Soybean Disease Biotechnology Center at the University of Illinois; United Soybean Board [1289] FX This work was partially funded by the Illinois Soybean Association, the Soybean Disease Biotechnology Center at the University of Illinois and United Soybean Board project #1289. We would like to thank many undergraduate students who helped in immature seed rescue program. We are thankful to Mr. Chong You who located perennial Glycine accessions on the map of Australia and Papua New Guinea. Professor G. H. Chung, Chonnam National University, Yeosu, Korea and Professor Jai Dev, C.S.K. Himanchal Pradesh Agricultural University, Palampur, India, who participated in this study. We are thankful to Raji Yusuf for taking care of plants in the greenhouse. NR 42 TC 3 Z9 3 U1 2 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2015 VL 128 IS 6 BP 1117 EP 1136 DI 10.1007/s00122-015-2494-0 PG 20 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CI3FU UT WOS:000354633800010 PM 25835560 ER PT J AU Cook, D Welch, KD Green, BT Gardner, DR Pfister, JA Constantino, JR Stonecipher, CA AF Cook, Daniel Welch, Kevin D. Green, Benedict T. Gardner, Dale R. Pfister, James A. Constantino, John R. Stonecipher, Clinton A. TI The relative toxicity of Delphinium stachydeum in mice and cattle SO TOXICON LA English DT Article DE Delphinium; Toxic plant; Norditerpene alkaloids; Cattle ID TALL LARKSPUR DELPHINIUM; NORDITERPENOID ALKALOIDS; DITERPENOID ALKALOIDS; SPP.; RANGELANDS; BARBEYI AB Larkspurs (Delphinium spp.) are poisonous plants on rangelands throughout the Western United States and Canada. Larkspur-induced poisoning in cattle is due to norditerpene alkaloids that are represented by two main structural groups of norditerpene alkaloids, the N-(methylsuccinimido) anthranoyllycoctonine type (MSAL-type) and the non-MSAL type. Information on the alkaloid composition and resulting toxicity in mice and cattle is lacking for a number of Delphinium species, including Delphinium stachydeum. The objective of this study was to determine the alkaloid composition of D. stachydeum and to characterize its relative toxicity in mice and cattle compared to two reference species Delphinium barbeyi and Delphinium occidentale. D. stachydeum contains the non-MSAL-type alkaloids but not the MSAL-type alkaloids. D. stachydeum was less toxic than D. barbeyi and D. occidentale in the mouse model. D. stachydeum was less toxic than the MSAL-containing D. barbeyi but much more toxic than the non-MSAL-containing D. occidentale in cattle as measured by heart rate and time of exercise. These results indicate that predictions of Delphinium toxicity can't be accurately made based solely on results from the mouse model or the absence of the MSAL-type alkaloids in the plant. Published by Elsevier Ltd. C1 [Cook, Daniel; Welch, Kevin D.; Green, Benedict T.; Gardner, Dale R.; Pfister, James A.; Constantino, John R.; Stonecipher, Clinton A.] USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Cook, D (reprint author), USDA, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov NR 27 TC 4 Z9 4 U1 0 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD JUN 1 PY 2015 VL 99 BP 36 EP 43 DI 10.1016/j.toxicon.2015.03.005 PG 8 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA CI8MD UT WOS:000355025500005 PM 25772859 ER EF