FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Lin, FM Marcelo, KL Rajapakshe, K Coarfa, C Dean, A Wilganowski, N Robinson, H Sevick, E Bissig, KD Goldie, LC Means, AR York, B AF Lin, Fumin Marcelo, Kathrina L. Rajapakshe, Kimal Coarfa, Cristian Dean, Adam Wilganowski, Nathaniel Robinson, Holly Sevick, Eva Bissig, Karl-Dimiter Goldie, Lauren C. Means, Anthony R. York, Brian TI The camKK2/camKIV relay is an essential regulator of hepatic cancer SO HEPATOLOGY LA English DT Article ID PROTEIN-KINASE KINASE; HEPATOCELLULAR-CARCINOMA; PROSTATE-CANCER; LIVER-CANCER; ACTIVATION; MTOR; SURVIVAL; PHOSPHORYLATION; IDENTIFICATION; STATISTICS AB Hepatic cancer is one of the most lethal cancers worldwide. Here, we report that the expression of Ca2+/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is significantly up-regulated in hepatocellular carcinoma (HCC) and negatively correlated with HCC patient survival. The CaMKK2 protein is highly expressed in all eight hepatic cancer cell lines evaluated and is markedly up-regulated relative to normal primary hepatocytes. Loss of CaMKK2 function is sufficient to inhibit liver cancer cell growth, and the growth defect resulting from loss of CaMKK2 can be rescued by ectopic expression of wild-type CaMKK2 but not by kinase-inactive mutants. Cellular ablation of CaMKK2 using RNA interference yields a gene signature that correlates with improvement in HCC patient survival, and ablation or pharmacological inhibition of CaMKK2 with STO-609 impairs tumorigenicity of liver cancer cells in vivo. Moreover, CaMKK2 expression is up-regulated in a time-dependent manner in a carcinogen-induced HCC mouse model, and STO-609 treatment regresses hepatic tumor burden in this model. Mechanistically, CaMKK2 signals through Ca2+/calmodulin-dependent protein kinase 4 (CaMKIV) to control liver cancer cell growth. Further analysis revealed that CaMKK2 serves as a scaffold to assemble CaMKIV with key components of the mammalian target of rapamycin/ribosomal protein S6 kinase, 70 kDa, pathway and thereby stimulate protein synthesis through protein phosphorylation. Conclusion: The CaMKK2/CaMKIV relay is an upstream regulator of the oncogenic mammalian target of rapamycin/ribosomal protein S6 kinase, 70 kDa, pathway, and the importance of this CaMKK2/CaMKIV axis in HCC growth is confirmed by the potent growth inhibitory effects of genetically or pharmacologically decreasing CaMKK2 activity; collectively, these findings suggest that CaMKK2 and CaMKIV may represent potential targets for hepatic cancer. (Hepatology 2015;62:505-520 C1 [Lin, Fumin; Marcelo, Kathrina L.; Rajapakshe, Kimal; Coarfa, Cristian; Dean, Adam; Bissig, Karl-Dimiter; Means, Anthony R.; York, Brian] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Wilganowski, Nathaniel; Robinson, Holly; Sevick, Eva] Univ Texas Hlth Sci Ctr Houston, Houston, TX 77030 USA. [Wilganowski, Nathaniel; Robinson, Holly; Sevick, Eva] Inst Mol Med, Ctr Mol Imaging, Houston, TX USA. [Bissig, Karl-Dimiter; Means, Anthony R.; York, Brian] Baylor Coll Med, Dan L Duncan Canc Ctr, Houston, TX 77030 USA. [Bissig, Karl-Dimiter; Goldie, Lauren C.] Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA. [Goldie, Lauren C.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Goldie, Lauren C.] ARS, USDA, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. RP York, B (reprint author), Baylor Coll Med, Dept Mol & Cellular Biol, 1 Baylor Plaza, Houston, TX 77030 USA. EM york@bcm.edu RI Rajapakshe, Kimal/P-1592-2015; Sevick-Muraca, Eva/A-4152-2017 OI Sevick-Muraca, Eva/0000-0002-8152-4847 FU NIGMS NIH HHS [R01 GM033976] NR 29 TC 8 Z9 8 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0270-9139 EI 1527-3350 J9 HEPATOLOGY JI Hepatology PD AUG PY 2015 VL 62 IS 2 BP 505 EP 520 DI 10.1002/hep.27832 PG 16 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA CN5EX UT WOS:000358453700022 PM 25847065 ER PT J AU Feda, DM Seelbinder, A Baek, S Raja, S Yin, L Roemmich, JN AF Feda, D. M. Seelbinder, A. Baek, S. Raja, S. Yin, L. Roemmich, J. N. TI Neighbourhood parks and reduction in stress among adolescents: Results from Buffalo, New York SO INDOOR AND BUILT ENVIRONMENT LA English DT Article DE Perceived stress; Adolescent; Neighbourhood environment; Parks; Physical activity ID TIME PHYSICAL-ACTIVITY; PERCEIVED STRESS; MENTAL-HEALTH; LEISURE-TIME; LONGITUDINAL ANALYSIS; NOISE EXPOSURE; GREEN SPACE; LIFE EVENTS; CHILDREN; ENVIRONMENTS AB Planners and landscape architects have long recognized the critical role of green space in urban environments. This cross-sectional field study of 68 adolescents determined the association between percent neighbourhood park area and perceived stress among adolescents, while controlling for physical activity. This study is the first to examine this association using objective measures of park area and adolescents' physical activity. A multivariate regression model indicated that percentage of park area ( = -62.573, p < 0.03) predicts perceived stress among adolescents. Access to neighbourhood parks buffers adolescents against perceived stress after controlling for socio-economic status and physical activity. Policy recommendations for incorporating parks into neighbourhood design are given. C1 [Feda, D. M.; Seelbinder, A.] SUNY Buffalo, Dept Pediat, Sch Med & Biomed Sci, Buffalo, NY 14214 USA. [Baek, S.; Raja, S.; Yin, L.] SUNY Buffalo, Sch Architecture & Planning, Dept Urban & Reg Planning, Buffalo, NY 14214 USA. [Roemmich, J. N.] USDA, Grand Forks Human Nutr Ctr, Grand Forks, ND 58203 USA. RP Feda, DM (reprint author), SUNY Buffalo, Dept Pediat, Bldg 26,Farber Hall,Room G56,3435 Main St, Buffalo, NY 14214 USA. EM dmfeda@buffalo.edu FU National Institutes of Health [HD055270] FX DMF implemented the protocol, collected data, drafted the manuscript, analysed and interpreted the data, and prepared the tables. AS collected data and revised the manuscript. SB computed the built environment variables, prepared the figure and revised the manuscript. SR and LY contributed to the study design and revised the manuscript. JNR developed the study concept and design, supervised acquisition of data, analysed and interpreted the data in collaboration with DMF, and revised previous and the final versions of the manuscript. All authors approved the final version of the manuscript for submission. None of the authors declared had conflicts of interest with respect to their authorship or the publication of this article. We would also like to thank Maya Lambiase, and Christina Lobarinas for their data collection, and Alex Brian Ticoalu for data process efforts. This work was supported by National Institutes of Health grant [HD055270] to JNR. The funding agency played no role in data collection, analysis or publication. NR 58 TC 0 Z9 0 U1 9 U2 25 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1420-326X EI 1423-0070 J9 INDOOR BUILT ENVIRON JI Indoor Built Environ. PD AUG PY 2015 VL 24 IS 5 BP 631 EP 639 DI 10.1177/1420326X14535791 PG 9 WC Construction & Building Technology; Engineering, Environmental; Public, Environmental & Occupational Health SC Construction & Building Technology; Engineering; Public, Environmental & Occupational Health GA CN6BJ UT WOS:000358518100006 ER PT J AU Kulas, M Porto-Fett, ACS Swartz, RS Shane, LE Strasser, H Munson, M Shoyer, BA Luchansky, JB AF Kulas, Megan Porto-Fett, Anna C. S. Swartz, Richard S. Shane, Laura E. Strasser, Hannah Munson, Madison Shoyer, Bradley A. Luchansky, John B. TI Thermal Inactivation of Shiga Toxin-Producing Escherichia coli Cells within Veal Cordon Bleu SO JOURNAL OF FOOD SAFETY LA English DT Article ID TENDERIZED BEEF STEAKS; FLAME GAS GRILL; ANTIMICROBIAL RESISTANCE; COOKING APPLIANCES; SOUTHERN ONTARIO; GROUND-BEEF; O157H7; O157/H7; SALMONELLA; SURVIVAL AB Veal cutlets were surface inoculated with ca. 6.6cfu/g of an eight-strain rifampicin-resistant cocktail of Shiga toxin-producing Escherichia coli (STEC) (O26:H11, O45:H2, O103:H2, O104:H4, O111:H-, O121:H19, O145:NM and O157:H7). Cutlets were mechanically tenderized and cordon bleu was prepared by adding slices of ham and cheese between two cutlets prior to batter/breading and cooking. Fully assembled cordon bleu were cooked in preheated (191.5C) extra virgin olive oil (45mL) on a griddle. Cooking for 4, 5 or 6min per side reduced STEC levels by ca. 1.3, 2.2 or 3.4logcfu/g, respectively, whereas cooking for 7-10min per side resulted in reductions of ca. 6.2logcfu/g. These data validated that cooking tenderized veal cordon bleu for at least 7 min per side in 45mL of olive oil on a griddle maintained at ca. 191.5C is sufficient to achieve an internal cordon bleu temperature of 69.03.3C and a 5-log reduction of STEC. Practical ApplicationsVeal cordon bleu is a popular meal prepared by placing a slice of cheese and a slice of cured pork between two cutlets and then coating/breading the resulting turnover-type product. We evaluated time and temperature cooking regimens for lethality toward Shiga toxin-producing Escherichia coli (STEC) inoculated onto mechanically tenderized multicomponent veal cordon bleu. Our results confirmed that a 5-log reduction of STEC was achieved by cooking veal cordon bleu to an internal temperature of 69.0 +/- 3.3C for at least 7min per side on a griddle maintained at 191.5C. Reductions of ca. 1.5-3.5logcfu/g were also achieved following cooking for 4 to 6min per side. These data establish guidelines for cooking veal cordon bleu, which, in turn, may decrease the potential risk of STEC illnesses associated with this product if pathogens are present and if these products are undercooked or mishandled. C1 [Kulas, Megan] Kansas State Univ, Manhattan, KS 66506 USA. [Porto-Fett, Anna C. S.; Shane, Laura E.; Shoyer, Bradley A.; Luchansky, John B.] USDA Agr Res Serv, Wyndmoor, PA 19038 USA. [Swartz, Richard S.] Penn State Univ, University Pk, PA 16802 USA. [Strasser, Hannah] Delaware Valley Coll, Doylestown, PA USA. [Munson, Madison] Drexel Univ, Philadelphia, PA 19104 USA. RP Luchansky, JB (reprint author), USDA Agr Res Serv, Wyndmoor, PA 19038 USA. EM John.Luchansky@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2012-68003-30155] FX We extend our sincere appreciation to John Phillips, Nelly Osoria, Ashley Hallowell and Laura Stahler (USDA/ARS/ERRC, Wyndmoor, PA) for their assistance on this project. This project was supported, in part, by Agriculture and Food Research Initiative Competitive Grant No. 2012-68003-30155 from the USDA National Institute of Food and Agriculture. NR 33 TC 1 Z9 1 U1 3 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0149-6085 EI 1745-4565 J9 J FOOD SAFETY JI J. Food Saf. PD AUG PY 2015 VL 35 IS 3 BP 403 EP 409 DI 10.1111/jfs.12188 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CN0TJ UT WOS:000358126300014 ER PT J AU Wang, YW Ding, W Kou, LP Li, L Wang, C Jurick, WM AF Wang, Yanwen Ding, Wu Kou, Liping Li, Liang Wang, Chen Jurick, Wayne M., II TI A Non-destructive method to assess freshness of raw bovine milk using FT-NIR spectroscopy SO JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE LA English DT Article DE Non-destructive evaluation; Raw milk; Freshness assessment; FT-NIR ID BACTERIA; PCA; PLS AB A non-destructive method to analyze the freshness of raw milk was developed using a FT-NIR spectrometer and a fiber optic probe. Diffuse transmittance spectra were acquired in the spectral range 833 similar to 2,500 nm from raw milk samples collected from Northwest A&F University Animal Husbandry Station. After each spectral acquisition, quality parameters such as acidity, pH, and lactose content were measured by traditional detection methods. For all milk samples, PLS (partial least square regression), MLR (multiple linear regression), and ANN (artificial neural networks) analyses were carried out in order to develop models to predict parameters that were indicative of freshness. Predictive models showed R-2 values up to 0.9647, 0.9876 and 0.8772 for acidity, pH, and lactose content, respectively (validation set validations). The similarity analysis and classification between raw milk freshness during storage was also conducted by means of hierarchical cluster analysis. Over an 8 day storage period, the highest heterogeneity was evident between days 1 and 2. C1 [Wang, Yanwen; Ding, Wu; Kou, Liping; Li, Liang; Wang, Chen] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Jurick, Wayne M., II] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. RP Ding, W (reprint author), Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. EM dingwu10142000@hotmail.com FU Natural Science Foundation of Shaanxi China [K332020916]; program for Northwest A F University [3-45] FX The authors gratefully acknowledge the financial support provided by the Natural Science Foundation of Shaanxi China (Grant No. K332020916) and the program for Northwest A & F University (Grant No. 3-45) NR 12 TC 1 Z9 1 U1 4 U2 22 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0022-1155 EI 0975-8402 J9 J FOOD SCI TECH MYS JI J. Food Sci. Technol.-Mysore PD AUG PY 2015 VL 52 IS 8 BP 5305 EP 5310 DI 10.1007/s13197-014-1574-5 PG 6 WC Food Science & Technology SC Food Science & Technology GA CN8CR UT WOS:000358665800069 PM 26243957 ER PT J AU Yen, H Sharifi, A Kalin, L Mirhosseini, G Arnold, JG AF Yen, Haw Sharifi, Amirreza Kalin, Latif Mirhosseini, Golbahar Arnold, Jeffrey G. TI Assessment of model predictions and parameter transferability by alternative land use data on watershed modeling SO JOURNAL OF HYDROLOGY LA English DT Article DE Alternative land use data; Input uncertainty; SWAT; IPEAT ID ASSESSMENT-TOOL; SPATIAL-RESOLUTION; OUTPUT UNCERTAINTY; CHESAPEAKE BAY; COVER DATABASE; UNITED-STATES; INPUT DATA; QUALITY; SWAT; SOIL AB In recent years, complex large-scale watershed models have been developed to perform simulations of hydrologic and nutrient processes. The potential impact caused by human activities such as agricultural implementations against the environment can be evaluated under future scenarios. Meanwhile, large amount of input data are required to enhance the performance of simulated results. For some natural or urban regions, it is possible to have multiple sources of geophysical data available but the associated effects of using alternating data sources on modeling results is not yet evaluated. In this study, three sources of land use data (Mid-Atlantic Regional Earth Science Applications Center (RESAC 2000), National Land Use Cover Dataset (NLCD 2001), and State Land Use/Cover Maps (STATE) were implemented on the Greensboro watershed, Maryland, USA. The Alternative Dataset Scheme (ADS) and the Parameter Transferability Scheme (PTS) were applied to investigate model predictive uncertainty and the potential impact of cross transferring optimal calibration parameters between models. It was demonstrated that model predictions simulated by SWAT model had better performance when RESAC land use map was used, followed by STATE, and NLCD land use maps. In addition, calibrated best parameter set from RESAC has presented relatively more transferable compared to NLCD and STATE. The use of varying data source may not only alter model predictions and the associated predictive uncertainty but also have direct impact on the transferability of model parameters. The major findings in this study may help future modelers and decision makers to recognize the importance of alternative data source selection. Therefore, the quality of subsequent research work, engineering applications or policies can be further improved. (C) 2015 Elsevier B.V. All rights reserved. C1 [Yen, Haw; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Yen, Haw] Texas A&M Univ, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Sharifi, Amirreza] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Kalin, Latif] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Kalin, Latif] Canik Basari Univ, Dept Civil Engn, TR-55080 Gurgenyatak Koyu, Canik Samsun, Turkey. [Mirhosseini, Golbahar] Auburn Univ, Biosyst Engn Dept, Auburn, AL 36849 USA. RP Yen, H (reprint author), Texas A&M Univ, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM hyen@brc.tamus.edu FU NOAA/UNH Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET); United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components FX This project was partially funded by grants from (i) NOAA/UNH Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET); and (ii) United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components. Other sources of funding were provided by the Center for Environmental Studies at the Urban-Rural Interface, Auburn University. Awesome comments provided by the associate editor and reviewer greatly improved the quality of the manuscript. Thanks a lot! In addition, please remember that USDA is an equal opportunity employer and provider. NR 52 TC 4 Z9 4 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG PY 2015 VL 527 BP 458 EP 470 DI 10.1016/j.jhydrol.2015.04.076 PG 13 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CN7QM UT WOS:000358629100040 ER PT J AU Ford, WI Fox, JF Pollock, E Rowe, H Chakraborty, S AF Ford, William I. Fox, James F. Pollock, Erik Rowe, Harold Chakraborty, Suvankar TI Testing assumptions for nitrogen transformation in a low-gradient agricultural stream SO JOURNAL OF HYDROLOGY LA English DT Article DE Stream nitrogen cycle; Agricultural watersheds; Surficial fine-grained laminae; Empirical mode decomposition; Autotrophic production ID NONSTATIONARY TIME-SERIES; ORGANIC-CARBON; SEDIMENT; DENITRIFICATION; PHOSPHORUS; CATCHMENTS; SOILS; TEMPERATURE; WATERSHEDS; ADSORPTION AB Common assumptions of the nitrogen cycle in agricultural streams point to biologic transformation of nitrate being tightly linked to benthic carbon turnover within fine sediments of the streambed. While the nitrogen and carbon linkage has been supported in agricultural streams using multi-week stream injection studies, few studies have tested these assumptions using multi-year time-series of carbon and nutrient species. We made elemental and isotopic measurements from 8 years of weekly suspended sediment samples on the main-stem, 14 months of dissolved nitrate samples on the main-stem and tributaries, and point observations of sediment samples from benthic algae and stream banks in an agricultural stream to test the assumptions. Results from Empirical Mode Decomposition of carbon and nitrogen time series suggest agreement with the prevailing assumption and coupling of benthic carbon dynamics with nitrate from late spring through fall during the 8 year sampling period for the temperate stream. During late spring, summer and fall, autotrophic growth and organic matter decomposition assist with controlling temporary sequestration of nitrate and denitrification in stream sediments, respectively. Contrary to conventional wisdom, our data results suggest decoupling of carbon and nitrogen dynamics from winter through mid-spring for much of the 8 year sampling period. During the winter and spring, nitrate loadings from upland fertilizer application and delivery of upland sediments by storm events are shown to instantaneously increase sediment nitrogen and instantaneously decrease transported nitrate. The result is attributed to abiotic transfer of transient nitrate storage during the winter and early spring due to variably charged sesquioxides within streambed sediments. The results provide the first study, to our knowledge, of the potential importance of nitrate sorption in the stream nitrogen cycle and potential implications are that sorption could retard nitrate loadings from downstream transport and increase the potential for denitrification beyond which would be expected with purely biologic based assumptions. Published by Elsevier B.V. C1 [Ford, William I.] USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. [Fox, James F.] Univ Kentucky, Dept Civil Engn, Lexington, KY 40506 USA. [Pollock, Erik] Univ Arkansas, Stable Isotope Lab, Fayetteville, AR 72701 USA. [Rowe, Harold] Univ Texas Austin, Bur Econ Geol, Austin, TX USA. [Chakraborty, Suvankar] Univ Utah, SIRFER, Salt Lake City, UT USA. RP Ford, WI (reprint author), USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. EM william.ford@ars.usda.gov OI Rowe, Harry/0000-0002-1665-6261; pollock, erik/0000-0002-2834-4935 FU University Of Kentucky Department Of Civil Engineering; National Science Foundation [0918856]; Kentucky Science & Engineering Foundation [2687-RDE-015] FX We thank Jason Backus with the Kentucky Geological Survey Laboratory for analysis of the concentration data and numerous undergraduate and graduate research associates that assisted with data collection and analysis. We thank the University Of Kentucky Department Of Civil Engineering for partial funding of the graduate student while at UK. We gratefully acknowledge financial support of this research under National Science Foundation Award #0918856 and Kentucky Science & Engineering Foundation Award #2687-RDE-015. NR 65 TC 4 Z9 4 U1 9 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG PY 2015 VL 527 BP 908 EP 922 DI 10.1016/j.jhydrol.2015.05.062 PG 15 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CN7QM UT WOS:000358629100077 ER PT J AU Dlott, G Maul, JE Buyer, J Yarwood, S AF Dlott, Glade Maul, Jude E. Buyer, Jeffrey Yarwood, Stephanie TI Microbial rRNA:rDNA gene ratios may be unexpectedly low due to extracellular DNA preservation in soils SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Activity; eDNA; iDNA; Oligotrophy; Preservation ID 16S RIBOSOMAL-RNA; COMMUNITY COMPOSITION; MARINE-SEDIMENTS; BACTERIAL; EXTRACTION; SEQUENCES; DYNAMICS; ARCHAEA; MICROORGANISMS; DECOMPOSITION AB We tested a method of estimating the activity of detectable individual bacterial and archaeal OTUs within a community by calculating ratios of absolute 16S rRNA to rDNA copy numbers. We investigated phylogenetically coherent patterns of activity among soil prokaryotes in non-growing soil communities. 'Activity ratios' were calculated for bacteria and archaea in soil sampled from a tropical rainforest and temperate agricultural field and incubated for one year at two levels of moisture availability and with and without carbon additions. Prior to calculating activity ratios, we corrected the relative abundances of OTUs to account for multiple copies of the 16S gene per genome. Although necessary to ensure accurate activity ratios, this correction did not change our interpretation of differences in microbial community composition across treatments. Activity ratios in this study were lower than those previously published (0.0003-210, logarithmic mean = 0.24), suggesting significant extracellular DNA preservation. After controlling for the influence of individual incubation jars, significant differences in activity ratios between all members of each phylum were observed. Planctomycetes and Firmicutes had the highest activity ratios and Crenarchaeota had the lowest activity overall. Our results suggest that greater caution should be taken in interpreting soil microbial community data derived from extracted DNA. Indirect extraction methods may be useful in ensuring that microbes identified from extracellular DNA are not erroneously interpreted as components of an active microbial community. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dlott, Glade; Yarwood, Stephanie] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Maul, Jude E.; Buyer, Jeffrey] ARS, Sustainable Agr Syst Lab, USDA, Beltsville, MD 20705 USA. RP Yarwood, S (reprint author), Univ Maryland, Dept Environm Sci & Technol, HJ Patterson Hall,Room 1216, College Pk, MD 20742 USA. EM gdlott@umd.edu; Jude.Maul@ARS.USDA.GOV; Jeffrey.Buyer@ARS.USDA.GOV; syarwood@umd.edu OI Buyer, Jeffrey/0000-0003-2098-0547 NR 66 TC 6 Z9 6 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD AUG PY 2015 VL 115 BP 112 EP 120 DI 10.1016/j.mimet.2015.05.027 PG 9 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA CN7PB UT WOS:000358625400022 PM 26055315 ER PT J AU Leone, A Chang, S Jirka, B AF Leone, Angela Chang, Sarah Jirka, Barbara TI Bring Nutrition Education to Life in the Classroom with MyPlate SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Leone, Angela; Chang, Sarah; Jirka, Barbara] USDA, Off Nutr Mkt & Commun, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. RP Leone, A (reprint author), USDA, Off Nutr Mkt & Commun, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 0 TC 0 Z9 1 U1 0 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD AUG PY 2015 VL 115 IS 8 BP 1200 EP 1202 DI 10.1016/j.jand.2015.06.003 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CN6IQ UT WOS:000358538700002 PM 26210083 ER PT J AU Espeland, MA Newman, AB Sink, K Gill, TM King, AC Miller, ME Guralnik, J Katula, J Church, T Manini, T Reid, KF McDermott, MM AF Espeland, Mark A. Newman, Anne B. Sink, Kaycee Gill, Thomas M. King, Abby C. Miller, Michael E. Guralnik, Jack Katula, Jeff Church, Timothy Manini, Todd Reid, Kieran F. McDermott, Mary M. CA LIFE Study Grp TI Associations Between Ankle-Brachial Index and Cognitive Function: Results From the Lifestyle Interventions and Independence for Elders Trial SO JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION LA English DT Article DE Cognitive function; ankle-brachial index; peripheral artery disease; dementia ID LOWER-EXTREMITY FUNCTION; PULSE-WAVE VELOCITY; CARDIOVASCULAR HEALTH; ALZHEIMERS-DISEASE; EDINBURGH ARTERY; OLDER-ADULTS; RISK-FACTORS; DIAGNOSTIC GUIDELINES; NATIONAL INSTITUTE; FOLLOW-UP AB Objective: The objective of this study was to evaluate cross-sectional and longitudinal associations between ankle-brachial index (ABI) and indicators of cognitive function. Design: Randomized clinical trial (Lifestyle Interventions and Independence for Elders Trial). Setting: Eight US academic centers. Participants: A total of 1601 adults ages 70-89 years, sedentary, without dementia, and with functional limitations. Measurements: Baseline ABI and interviewer-and computer-administered cognitive function assessments were obtained. These assessments were used to compare a physical activity intervention with a health education control. Cognitive function was reassessed 24 months later (interviewer-administered) and 18 or 30 months later (computer-administered) and central adjudication was used to classify individuals as having mild cognitive impairment, probable dementia, or neither. Results: Lower ABI had a modest independent association with poorer cognitive functioning at baseline (partial r = 0.09; P < .001). Although lower baseline ABI was not associated with overall changes in cognitive function test scores, it was associated with higher odds for 2-year progression to a composite of either mild cognitive impairment or probable dementia (odds ratio 2.60 per unit lower ABI; 95% confidence interval 1.06-6.37). Across 2 years, changes in ABI were not associated with changes in cognitive function. Conclusion: In an older cohort sedentary individuals with dementia and with functional limitations, lower baseline ABI was independently correlated with cognitive function and associated with greater 2-year risk for progression to mild cognitive impairment or probable dementia. (C) 2015 AMDA - The Society for Post-Acute and Long-Term Care Medicine. C1 [Espeland, Mark A.; Miller, Michael E.] Wake Forest Sch Med, Dept Biostat Sci, Winston Salem, NC 27157 USA. [Newman, Anne B.] Univ Pittsburgh, Hlth Aging Res Program, Pittsburgh, PA USA. [Sink, Kaycee] Wake Forest Sch Med, J Paul Sticht Ctr Aging, Winston Salem, NC 27157 USA. [Gill, Thomas M.] Yale Univ, Sch Med, Dept Med, New Haven, CT 06510 USA. [King, Abby C.] Stanford Univ, Sch Med, Dept Hlth & Res Policy, Stanford, CA 94305 USA. [King, Abby C.] Stanford Univ, Dept Med, Sch Med, Stanford Prevent Res Ctr, Stanford, CA 94305 USA. [Guralnik, Jack] Univ Maryland, Dept Epidemiol & Publ Hlth, Baltimore, MD 21201 USA. [Katula, Jeff] Wake Forest Univ, Dept Hlth & Exercise Sci, Winston Salem, NC 27109 USA. [Church, Timothy] Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Manini, Todd] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL USA. [Reid, Kieran F.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [McDermott, Mary M.] Northwestern Univ, Dept Med, Feinberg Sch Med, Chicago, IL 60611 USA. RP Espeland, MA (reprint author), Wake Forest Sch Med, Dept Biostat Sci, Med Ctr Blvd, Winston Salem, NC 27157 USA. EM mespelan@wakehealth.edu RI Buford, Thomas /B-1111-2011; Newman, Anne B./C-6408-2013; OI Buford, Thomas /0000-0002-9541-4358; Marsiske, Michael/0000-0001-5973-2116; Newman, Anne B./0000-0002-0106-1150; Glynn, Nancy/0000-0003-2265-0162 FU National Institutes of Health/National Institute on Aging Cooperative Agreement [UO1 AG22376]; National Heart, Lung and Blood Institute [3U01AG022376-05A2S]; Intramural Research Program, National Institute on Aging, NIH [1I01CX000927-01A1]; Claude D. Pepper Older Americans Independence Centers at the University of Florida [1 P30 AG028740]; Wake Forest University [1 P30 AG21332]; Tufts University [1P30AG031679]; University of Pittsburgh [P30 AG024827]; Yale University [P30AG021342]; National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Stanford University [UL1 RR025744]; Boston Rehabilitation Outcomes Cente [1R24HD065688-01A1]; National Institute on Aging [K07AG3587]; Department of Veterans Affairs; US Department of Agriculture [58-1950-7-707] FX The Lifestyle Interventions and Independence for Elders Study is funded by a National Institutes of Health/National Institute on Aging Cooperative Agreement #UO1 AG22376 and a supplement from the National Heart, Lung and Blood Institute 3U01AG022376-05A2S, and sponsored in part by the Intramural Research Program, National Institute on Aging (No. 1I01CX000927-01A1), NIH.; The research is partially supported by the Claude D. Pepper Older Americans Independence Centers at the University of Florida (1 P30 AG028740), Wake Forest University (1 P30 AG21332), Tufts University (1P30AG031679), University of Pittsburgh (P30 AG024827), and Yale University (P30AG021342) and the National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Stanford University (UL1 RR025744). Tufts University is also supported by the Boston Rehabilitation Outcomes Center (1R24HD065688-01A1). LIFE investigators are also partially supported by the following:; TMG (Yale University) is the recipient of an Academic Leadership Award (K07AG3587) from the National Institute on Aging.; Dr Carlos Fragoso (Spirometry Reading Center, Yale University) is the recipient of a Career Development Award from the Department of Veterans Affairs.; Dr Roger Fielding (Tufts University) is partially supported by the US Department of Agriculture, under agreement 58-1950-7-707. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. NR 39 TC 1 Z9 1 U1 1 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1525-8610 EI 1538-9375 J9 J AM MED DIR ASSOC JI J. Am. Med. Dir. Assoc. PD AUG 1 PY 2015 VL 16 IS 8 BP 682 EP 689 DI 10.1016/j.jamda.2015.03.010 PG 8 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA CN4TP UT WOS:000358423400010 PM 25869993 ER PT J AU Sollmann, R White, AM Gardner, B Manley, PN AF Sollmann, Rahel White, Angela M. Gardner, Beth Manley, Patricia N. TI Investigating the effects of forest structure on the small mammal community in frequent-fire coniferous forests using capture-recapture models for stratified populations SO MAMMALIAN BIOLOGY LA English DT Article DE Abundance; Distribution; Disturbance regime; Live trapping; Forest management ID FUEL-REDUCTION TREATMENTS; NORTHERN SIERRA-NEVADA; COARSE WOODY DEBRIS; OLD-GROWTH FORESTS; HABITAT ASSOCIATIONS; ANIMAL POPULATIONS; ECOLOGICAL TRAPS; SPATIAL SCALES; PINE FOREST; MICE AB Small mammals comprise an important component of forest vertebrate communities. Our understanding of how small mammals use forested habitat has relied heavily on studies in forest systems not naturally prone to frequent disturbances. Small mammal populations that evolved in frequent-fire forests, however, may be less restricted to specific habitat conditions due to the instability of these resources in time and space. We investigate how canopy cover and the volume of coarse woody debris (CWD), covariates that are considered important for small mammals, impact abundance and body mass of eight small mammal species. Based on live-trapping data collected across 23 sites over three years in a frequent fire forest in the Sierra Nevada we apply capture-recapture models for stratified populations, a statistically rigorous, rarely used framework that allows joint modeling of detection, abundance and its response to covariates. Canopy cover had a strong negative association with the abundance of yellow-pine chipmunks and California ground squirrels, and a strong positive association with deer mice. CWD had a strong negative association with the abundance of golden-mantled ground squirrels, yellow-pine and long-eared chipmunks, and a strong positive association with deer mice. Whereas canopy cover influenced abundance and body mass similarly, CWD had a positive association with body mass and a negative association with abundance in some species. These patterns could arise if suitable habitat is monopolized by socially dominant individuals. Despite these habitat associations, the small mammal community in our study was dynamic and diverse, with spatial and temporal variation in dominant species suggesting that species were flexible in their use of habitat. This study suggests that it is important to understand the disturbance regimes when investigating habitat requirements, coexistence and evolutionary ecology of small mammal species. (C) 2015 Deutsche Gesellschaft fur Saugetierkunde. Published by Elsevier GmbH. All rights reserved. C1 [Sollmann, Rahel; Gardner, Beth] N Carolina State Univ, Dept Forestry & Environm Resources, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. [White, Angela M.; Manley, Patricia N.] US Forest Serv, Pacific Southwest Res Stn, Davis, CA USA. RP Sollmann, R (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Campus Box 8008, Raleigh, NC 27695 USA. EM rsollma@ncsu.edu FU US Forest Service Pacific Southwest Research Station; Sierra Nevada Public Lands Management Act FX This work was made possible by the hard work of our field crews, particularly Scott Appleby, Katrina Heckendorn and Amy Sturgill. Eric Newkirk and Tray Biasiolli assisted with database management. We thank the managers in the Lake Tahoe Basin Management Unit for their continued support. We further thank J. Andrew Royle and Sarah Converse for advice on stratified CR models, RENCI for access to a high performance computer when we explored spatial CR analysis of the data, and Takashi Saitoh and three anonymous reviewer for valuable comments on an earlier draft of this manuscript. Funding was provided by US Forest Service Pacific Southwest Research Station, and the Sierra Nevada Public Lands Management Act. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 61 TC 5 Z9 6 U1 20 U2 58 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1616-5047 EI 1618-1476 J9 MAMM BIOL JI Mamm. Biol. PD AUG PY 2015 VL 80 IS 4 BP 247 EP 254 DI 10.1016/j.mambio.2015.03.002 PG 8 WC Zoology SC Zoology GA CN5KU UT WOS:000358469000001 ER PT J AU Liu, WW Gray, S Huo, Y Li, L Wei, TY Wang, XF AF Liu, Wenwen Gray, Stewart Huo, Yan Li, Li Wei, Taiyun Wang, Xifeng TI Proteomic Analysis of Interaction between a Plant Virus and Its Vector Insect Reveals New Functions of Hemipteran Cuticular Protein SO MOLECULAR & CELLULAR PROTEOMICS LA English DT Article ID RICE-STRIPE-VIRUS; ENDOPLASMIC-RETICULUM MEMBRANES; LAODELPHAX-STRIATELLUS FALLEN; MULTIPLE SEQUENCE ALIGNMENT; YELLOW-DWARF-VIRUS; ANOPHELES-GAMBIAE; CUTICLE PROTEINS; HOMOTYPIC FUSION; SITOBION-AVENAE; MYZUS-PERSICAE AB Numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. Here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (Rice stripe virus, RSV, genus Tenuivirus) with its vector insect, small brown planthopper (Laodelphax striatellus). Sixty-six proteins of L. striatellus that interacted with the nucleocapsid protein (pc3) of RSV were identified. A virus-insect interaction network, constructed for pc3 and 29 protein homologs of Drosophila melanogaster, suggested that nine proteins might directly interact with pc3. Of the 66 proteins, five (atlasin, a novel cuticular protein, jagunal, NAC domain protein, and vitellogenin) were most likely to be involved in viral movement, replication, and transovarial transmission. This work also provides evidence that the novel cuticular protein, CPR1, from L. striatellus is essential for RSV transmission by its vector insect. CPR1 binds the nucleocapsid protein (pc3) of RSV both in vivo and in vitro and colocalizes with RSV in the hemocytes of L. striatellus. Knockdown of CPR1 transcription using RNA interference resulted in a decrease in the concentration of RSV in the hemolymph, salivary glands and in viral transmission efficiency. These data suggest that CPR1 binds RSV in the insect and stabilizes the viral concentration in the hemolymph, perhaps to protect the virus or to help move the virus to the salivary tissues. Our studies provide direct experimental evidence that viruses can use existing vector proteins to aid their survival in the hemolymph. Identifying these putative vector molecules should lead to a better understanding of the interactions between viruses and vector insects. C1 [Liu, Wenwen; Li, Li; Wang, Xifeng] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Gray, Stewart] Cornell Univ, USDA, ARS, Plant Protect Res Unit, Ithaca, NY USA. [Huo, Yan] Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100080, Peoples R China. [Huo, Yan] Fujian Agr & Forestry Univ, Inst Plant Virol, Fujian Prov Key Lab Plant Virol, Fuzhou 350002, Peoples R China. RP Wang, XF (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM xfwang@ippcaas.cn RI Wang, Xifeng/A-6476-2016 FU National Key Basic Research of China [2010CB126200]; Natural Science Foundation of China [NSFC31401713]; China Postdoctoral Science Foundation [2014M560145]; Special Fund for Agro-scientific Research in the Public Interest [201303021] FX This work was supported by the National Key Basic Research of China (2010CB126200), Natural Science Foundation of China (NSFC31401713), China Postdoctoral Science Foundation (2014M560145), and the Special Fund for Agro-scientific Research in the Public Interest (201303021). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 61 TC 13 Z9 15 U1 2 U2 30 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 1535-9476 EI 1535-9484 J9 MOL CELL PROTEOMICS JI Mol. Cell. Proteomics PD AUG PY 2015 VL 14 IS 8 BP 2229 EP 2242 DI 10.1074/mcp.M114.046763 PG 14 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CO0JR UT WOS:000358837400014 PM 26091699 ER PT J AU Sun, LJ Miao, ZY Cai, CM Zhang, DJ Zhao, MX Wu, YY Zhang, XL Swarm, SA Zhou, LW Zhang, ZYJ Nelson, RL Ma, JX AF Sun, Lianjun Miao, Zhenyan Cai, Chunmei Zhang, Dajian Zhao, Meixia Wu, Yanyan Zhang, Xueling Swarm, Stephen A. Zhou, Liwen Zhang, Zhanyuan J. Nelson, Randall L. Ma, Jianxin TI GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean SO NATURE GENETICS LA English DT Article ID DOMESTICATION-RELATED TRAITS; GLYCINE-MAX; SEED COATS; GENETIC-ANALYSIS; GRAIN LEGUMES; GENOME; WATER; DIVERSITY; SELECTION; WILD AB Loss of seed-coat impermeability was essential in the domestication of many leguminous crops to promote the production of their highly nutritious seeds. Here we show that seed-coat impermeability in wild soybean is controlled by a single gene, GmHs1-1, which encodes a calcineurin-like metallophosphoesterase transmembrane protein. GmHs1-1 is primarily expressed in the Malpighian layer of the seed coat and is associated with calcium content. The transition from impermeability to permeability in domesticated soybean was caused by artificial selection of a point mutation in GmHs1-1. Interestingly, a number of soybean landraces evaded selection for permeability because of an alternative selection for seed-coat cracking that also enables seed imbibition. Despite the single origin of the mutant allele Gmhs1-1, the distribution pattern of allelic variants in the context of soybean population structure and the detected signature of genomic introgression between wild and cultivated soybeans suggest that Gmhs1-1 may have experienced reselection for seed-coat permeability. C1 [Sun, Lianjun; Miao, Zhenyan; Zhang, Dajian; Zhao, Meixia; Ma, Jianxin] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Cai, Chunmei; Wu, Yanyan; Zhang, Xueling; Ma, Jianxin] Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China. [Swarm, Stephen A.; Nelson, Randall L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Zhou, Liwen; Zhang, Zhanyuan J.] Univ Missouri, Div Plant Sci, Plant Transformat Core Facil, Columbia, MO USA. [Nelson, Randall L.] USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL USA. RP Ma, JX (reprint author), Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. EM maj@purdue.edu RI ZHAO, MEIXIA/N-3124-2015 OI ZHAO, MEIXIA/0000-0001-8812-8217 FU Indiana Soybean Alliance [00049970, 14055567]; Taishan Scholarship and High-Level Talents Foundation of Qingdao Agricultural University [631304]; North Central Soybean Research Program [58-3611-4-017]; Ag Alumni Seed and the Purdue Agricultural Research Program FX We thank Z. Tian, J. Du, F. Lin, J. Ping, L. Zhu and A. Johnson for their involvement in developing the mapping populations at Purdue University, and we thank D. Szymanski for valuable comments on the manuscript. This work was mainly supported by soybean checkoff funds from the Indiana Soybean Alliance (00049970 and 14055567) and was partially supported by the Taishan Scholarship and High-Level Talents Foundation of Qingdao Agricultural University (631304), the North Central Soybean Research Program (58-3611-4-017), Ag Alumni Seed and the Purdue Agricultural Research Program. The US Department of Agriculture-Agricultural Research Service is an equal-opportunity provider and employer. NR 45 TC 5 Z9 5 U1 11 U2 37 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD AUG PY 2015 VL 47 IS 8 BP 939 EP + DI 10.1038/ng.3339 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA CN8FG UT WOS:000358674100021 PM 26098868 ER PT J AU Li, SM Banuelos, GS Min, J Shi, WM AF Li, Sumei Banuelos, Gary S. Min, Ju Shi, Weiming TI Effect of continuous application of inorganic nitrogen fertilizer on selenium concentration in vegetables grown in the Taihu Lake region of China SO PLANT AND SOIL LA English DT Article DE Field experiment; Inorganic N-fertilizer; Nitrate accumulation; Se availability; Vegetable Se concentration ID SOIL NUTRIENTS; WHEAT-GRAIN; PADDY SOIL; PLANTS; RICE; SPECIATION; SULFUR; FOOD; TRANSLOCATION; CONTAMINATION AB Vegetables provide an important source of selenium (Se) for many regions of China. Excess nitrogen (N) fertilizers are commonly used for vegetable production. In this regard, the effect of inorganic N-fertilizer application on the Se content in soils and plants was evaluated in vegetables produced in a polytunnel greenhouse vegetable system. Vegetables were continuously cultivated for 6 years in a microplot field experiment and treated annually with conventional N fertilizer (870 kg ha(-1) inorganic fertilizer) and 0 kg N ha(-1) as control treatment in polytunnel greenhouse vegetable system in the Taihu Lake region of China. Both treatments included 234 kg ha(-1) N provided by manure. After each harvest, soil and vegetable samples were collected, and analyzed for soil NO3-N, Olsen-P, Se, phosphate extractable S and Se and vegetable Se. Six years of continuous application of inorganic N-fertilizer resulted in a significant accumulation of NO3-N, Olsen-P and extractable S in the soil over the course of the experiment. Soil NO3-N concentration accumulated up to 600 mg kg(-1) with application of inorganic N-fertilizer, while soil phosphate extractable Se and vegetables Se concentration were significantly lower than that those grown in control treatments. The negative effect of continuous application of inorganic N-fertilizer on vegetable Se concentration may be a consequent of excessive accumulated soil NO3-N. When NO3-N concentration was greater than 300 mg kg(-1), vegetable Se concentration was negatively correlated with soil NO3-N. The negative effect of continuous application of inorganic N-fertilizer on vegetable Se concentrations was likely the result of soil nitrate's competitive or antagonistic effect on Se uptake by vegetables grown in an intensive polytunnel vegetable cultivation system. C1 [Li, Sumei; Min, Ju; Shi, Weiming] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. [Banuelos, Gary S.] ARS, San Joaquin Valley Agr Sci Ctr, USDA, Parlier, CA 93648 USA. RP Shi, WM (reprint author), Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. EM smli@issas.ac.cn; gary.banuelos@ars.usda.gov; jmin@issas.ac.cn; wmshi@issas.ac.cn FU National Science & Technology Pillar Program [2012BAD15B03] FX We are grateful to the key projects in the National Science & Technology Pillar Program in the Twelfth Five-year Plan (2012BAD15B03) for financial support. We thank Xihai Deng and Hua Gong for analyzing the Se concentration in soil and vegetable samples. NR 41 TC 2 Z9 2 U1 6 U2 50 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD AUG PY 2015 VL 393 IS 1-2 BP 351 EP 360 DI 10.1007/s11104-015-2496-3 PG 10 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA CN8CY UT WOS:000358666600027 ER PT J AU Camhi, SM Evans, EW Hayman, LL Lichtenstein, AH Must, A AF Camhi, Sarah M. Evans, E. Whitney Hayman, Laura L. Lichtenstein, Alice H. Must, Aviva TI Healthy eating index and metabolically healthy obesity in US adolescents and adults SO PREVENTIVE MEDICINE LA English DT Article DE Diet; Metabolically healthy obese (MHO); Obesity; HEI-2005 ID BODY-MASS INDEX; NUTRITION EXAMINATION SURVEY; 3RD NATIONAL-HEALTH; FOOD GROUP INTAKE; CARDIOVASCULAR-DISEASE; DIETARY PATTERNS; NORMAL-WEIGHT; RISK-FACTORS; PREVALENCE; PHENOTYPES AB Objective. To determine whether dietary quality differs between metabolically-healthy-obese (MHO) and metabolically-abnormal-obesity (MAO) in a nationally representative sample. Methods. National Health and Nutrition Examination Survey (NHANES) data (2007-2008; 2009-2010) were used to identify obese adolescents (>= 95th body mass index (BMI) %tile) and adults (>= 30 kg/m(2)). MHO was defined as <2 abnormal cardiometabolic risk factors (elevated blood pressure, triglycerides, glucose, low high density lipoprotein cholesterol (HDL-C); or on medications). Healthy Eating Index 2005 (HEI-2005) scores were calculated from 24-hour recall data. General linear regression models determined whether HEI-2005 scores differed between MHO and MAO after controlling for age, race, gender, NHANES wave, BMI, physical activity and health status by age group (12-18; 19-44; 45-85 years). Results. Compared with MAO, MHO adolescents (n = 133) had higher total HEI-2005 score, higher milk scores, and higher scores from calories from solid fats, alcohol beverages and added sugars. MHO women 19-44 years (n = 240) had higher total HEI-2005, higher whole fruit, higher whole grain and higher meat and bean scores compared with MAO. No significant differences were observed between MHO and MAO for HEI-2005 total scores in men 19-44 years, or adults 45-85 years. Conclusion. MHO adolescents and women 19-44 years have better dietary compliance to the U.S. guidelines when compared with MAO, suggesting potential intervention targets to improve cardiometabolic risk within obesity. (C) 2015 Elsevier Inc. All rights reserved. C1 [Camhi, Sarah M.] Univ Massachusetts, Exercise & Hlth Sci Dept, Boston, MA 02125 USA. [Evans, E. Whitney] Miriam Hosp, Brown Med Sch, Weight Control & Diabet Res Ctr, Providence, RI USA. [Hayman, Laura L.] Univ Massachusetts, Off Vice Provost Res, Boston, MA 02125 USA. [Lichtenstein, Alice H.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Must, Aviva] Tufts Univ, Sch Med, Dept Publ Hlth Community Med, Boston, MA 02111 USA. RP Camhi, SM (reprint author), Univ Massachusetts, Coll Nursing & Hlth Sci, Exercise & Hlth Sci Dept, 100 Morrissey Blvd, Boston, MA 02125 USA. EM sarah.camhi@umb.edu NR 46 TC 11 Z9 11 U1 1 U2 21 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0091-7435 EI 1096-0260 J9 PREV MED JI Prev. Med. PD AUG PY 2015 VL 77 BP 23 EP 27 DI 10.1016/j.ypmed.2015.04.023 PG 5 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA CN4HQ UT WOS:000358391600005 PM 25937589 ER PT J AU Hirschi, KD Pruss, GJ Vance, V AF Hirschi, Kendal D. Pruss, Gail J. Vance, Vicki TI Dietary delivery: a new avenue for microRNA therapeutics? SO TRENDS IN BIOTECHNOLOGY LA English DT Editorial Material ID SMALL RNAS; PLANTS AB Many people carefully monitor their food choices, adhering to the philosophy that 'you are what you eat'. Recent research adds a new wrinkle to that old adage, suggesting that dietary small RNAs (sRNAs) can control the gene expression of the consumer and may provide an effective, noninvasive, and inexpensive therapy for many human diseases. C1 [Hirschi, Kendal D.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Pruss, Gail J.; Vance, Vicki] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. RP Hirschi, KD (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM kendalh@bcm.edu FU NCI NIH HHS [R03 CA181895] NR 12 TC 6 Z9 7 U1 3 U2 14 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0167-7799 J9 TRENDS BIOTECHNOL JI Trends Biotechnol. PD AUG PY 2015 VL 33 IS 8 BP 431 EP 432 DI 10.1016/j.tibtech.2015.06.003 PG 2 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CN9XX UT WOS:000358805500002 PM 26113189 ER PT J AU Villar, JL Turland, NJ Juan, A Gaskin, JF Alonso, MA Crespo, MB AF Villar, Jose L. Turland, Nicholas J. Juan, Ana Gaskin, John F. Angeles Alonso, M. Crespo, Manuel B. TI Tamarix minoa (Tamaricaceae), a new species from the island of Crete (Greece) based on morphological and plastid molecular sequence data SO WILLDENOWIA LA English DT Article DE Mediterranean flora; Aegean flora; molecular phylogeny; taxonomy; Tamarix africana; Tamarix hampeana ID DNA; LIKELIHOOD; INFERENCE; MRBAYES; REGIONS; MODELS AB Tamarix minoa is described from material collected on the S Aegean island of Crete (Kriti), Greece. A morphological comparison with the species considered to be closest, T. africana and T. hampeana, is provided. An original illustration showing the main morphological characters of the new species is also given, as are photographs of the new species in its habitat. The isolated phylogenetic position of T. minoa is shown to be strongly supported by plastid molecular sequence data (trnS-trnG, trnQ-rps16 and ndhF-rpl32), thus warranting its recognition at specific rank. C1 [Villar, Jose L.; Juan, Ana; Angeles Alonso, M.; Crespo, Manuel B.] Univ Alicante, Inst Univ CIBIO, ES-03080 Alicante, Spain. [Villar, Jose L.; Juan, Ana; Angeles Alonso, M.; Crespo, Manuel B.] Univ Alicante, DCARN, ES-03080 Alicante, Spain. [Turland, Nicholas J.] Free Univ Berlin, Bot Garten, D-14195 Berlin, Germany. [Turland, Nicholas J.] Free Univ Berlin, Bot Museum Berlin, D-14195 Berlin, Germany. [Gaskin, John F.] USDA ARS, Pest Management Res Unit, Sidney, MT 59270 USA. RP Villar, JL (reprint author), Univ Alicante, Inst Univ CIBIO, POB 99, ES-03080 Alicante, Spain. EM jose.villar@ua.es; n.turland@bgbm.org; ana.juan@ua.es; john.gaskin@ars.usda.gov; ma.alonso@ua.es; crespo@ua.es RI Villar Garcia, Jose Luis/H-9372-2015 OI Villar Garcia, Jose Luis/0000-0003-1729-8010 FU National Geographic Society (NGS) [8573-08]; I+D+I project (Mo de Educacion y Ciencia, Spanish Government) [CGL2008-05056]; Mo de Agricultura, Alimentacion y Medio Ambiente, Spanish Government [OAPN 354-2011]; University of Alicante, Spain [ACIE10-01, ACIE11-05, ACIE13-08] FX We warmly thank Maria Garcia Rivas for drawing the illustration (Fig. 1); the curators and personnel of all the herbaria mentioned in the text, especially James Solomon (MO) for the loan of the isotype specimen conserved there; Kim Mann for her kindness during the lab work carried out in Sidney, MT, U.S.A.; Fernando Martinez Flores for his valuable comments and Elena Martinez for her help with the field work. One of us (N. Turland) thanks the National Geographic Society for supporting the research project "Human recreation versus plant diversity on maritime sands in Crete" (NGS grant no. 8573-08), during which the type gathering of Tamarix minoa was made, and Pepy Bareka (ACA) for participating in the field work. The FPU programme (Mo de Educacion, Spain), the I+D+I project CGL2008-05056 (Mo de Educacion y Ciencia, Spanish Government), the project OAPN 354-2011 (Mo de Agricultura, Alimentacion y Medio Ambiente, Spanish Government) and complementary supporting funds ACIE10-01, ACIE11-05 and ACIE13-08 (University of Alicante, Spain) made this research possible. NR 29 TC 0 Z9 0 U1 1 U2 2 PU BOTANISCHER GARTEN & BOTANISCHE MUSEUM BERLIN-DAHLEM PI BERLIN PA FREIE UNIV BERLIN, KOENIGIN-LUISE-STRASSE 6-8, BERLIN, D-14191, GERMANY SN 0511-9618 J9 WILLDENOWIA JI Willdenowia PD AUG PY 2015 VL 45 IS 2 BP 161 EP 172 DI 10.3372/wi.45.45201 PG 12 WC Plant Sciences SC Plant Sciences GA CN4ZG UT WOS:000358438500001 ER PT J AU Nachman, RJ Zhang, QR Denlinger, D Jiang, HB Park, Y AF Nachman, Ronald J. Zhang, Qirui Denlinger, David Jiang, Hongbo Park, Yoonseong TI Development of mimetic analogs of pyrokinin-like neuropeptides to disrupt pest insect physiology/behavior SO AMINO ACIDS LA English DT Meeting Abstract C1 [Nachman, Ronald J.] USDA, Insect Neuropeptide Lab, Insect Control & Cotton Dis Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Zhang, Qirui; Denlinger, David] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA. [Jiang, Hongbo; Park, Yoonseong] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RI Zhang, Qirui/C-6579-2011 OI Zhang, Qirui/0000-0002-2749-9740 NR 0 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0939-4451 EI 1438-2199 J9 AMINO ACIDS JI Amino Acids PD AUG PY 2015 VL 47 IS 8 BP 1635 EP 1636 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CN1JH UT WOS:000358175100081 ER PT J AU Davis, TA Columbus, DA Steinhoff-Wagner, J Suryawan, A Fiorotto, ML Nguyen, HV AF Davis, Teresa A. Columbus, Daniel A. Steinhoff-Wagner, Julia Suryawan, Agus Fiorotto, Marta L. Nguyen, Hanh V. TI Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs SO AMINO ACIDS LA English DT Meeting Abstract C1 [Davis, Teresa A.; Columbus, Daniel A.; Steinhoff-Wagner, Julia; Suryawan, Agus; Fiorotto, Marta L.; Nguyen, Hanh V.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0939-4451 EI 1438-2199 J9 AMINO ACIDS JI Amino Acids PD AUG PY 2015 VL 47 IS 8 BP 1642 EP 1642 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CN1JH UT WOS:000358175100101 ER PT J AU Wang, KX Mattoo, AK Handa, AK AF Wang, Kexin Mattoo, Autar K. Handa, Avtar K. TI RNAseq reveals polyamine signaling in relation to tomato fruit development and ripening SO AMINO ACIDS LA English DT Meeting Abstract C1 [Wang, Kexin; Handa, Avtar K.] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA. [Mattoo, Autar K.] ARS, Sustainable Agr Syst Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 3 U2 9 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0939-4451 EI 1438-2199 J9 AMINO ACIDS JI Amino Acids PD AUG PY 2015 VL 47 IS 8 BP 1666 EP 1667 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CN1JH UT WOS:000358175100171 ER PT J AU Snyder, AK Rio, RVM AF Snyder, Anna K. Rio, Rita V. M. TI "Wigglesworthia morsitans" Folate (Vitamin B-9) Biosynthesis Contributes to Tsetse Host Fitness SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID TRYPANOSOMA-BRUCEI-BRUCEI; SODALIS-GLOSSINIDIUS; BUCHNERA-APHIDICOLA; GENOME SEQUENCE; G-BREVIPALPIS; 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; SECONDARY ENDOSYMBIONT; DENSITY REGULATION; ESCHERICHIA-COLI; GENE-EXPRESSION AB Closely related ancient endosymbionts may retain minor genomic distinctions through evolutionary time, yet the biological relevance of these small pockets of unique loci remains unknown. The tsetse fly (Diptera: Glossinidae), the sole vector of lethal African trypanosomes (Trypanosoma spp.), maintains an ancient and obligate mutualism with species belonging to the gammaproteobacterium Wigglesworthia. Extensive concordant evolution with associated Wigglesworthia species has occurred through tsetse species radiation. Accordingly, the retention of unique symbiont loci between Wigglesworthia genomes may prove instrumental toward host species-specific biological traits. Genome distinctions between "Wigglesworthia morsitans" (harbored within Glossina morsitans bacteriomes) and the basal species Wigglesworthia glossinidia (harbored within Glossina brevipalpis bacteriomes) include the retention of chorismate and downstream folate (vitamin B-9) biosynthesis capabilities, contributing to distinct symbiont metabolomes. Here, we demonstrate that these W. morsitans pathways remain functionally intact, with folate likely being systemically disseminated through a synchronously expressed tsetse folate transporter within bacteriomes. The folate produced by W. morsitans is demonstrated to be pivotal for G. morsitans sexual maturation and reproduction. Modest differences between ancient symbiont genomes may still play key roles in the evolution of their host species, particularly if loci are involved in shaping host physiology and ecology. Enhanced knowledge of the Wigglesworthia-tsetse mutualism may also provide novel and specific avenues for vector control. C1 [Snyder, Anna K.; Rio, Rita V. M.] W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA. RP Rio, RVM (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. EM rita.rio@mail.wvu.edu FU NASA West Virginia Space Grant Consortium graduate fellowship; National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R01AI118789] FX This work was supported by a NASA West Virginia Space Grant Consortium graduate fellowship (to A.K.S.) and the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R01AI118789 (to R.V.M.R.). NR 95 TC 1 Z9 1 U1 3 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD AUG PY 2015 VL 81 IS 16 BP 5375 EP 5386 DI 10.1128/AEM.00553-15 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CN2UX UT WOS:000358278600011 PM 26025907 ER PT J AU Prochaska, TJ Donze-Reiner, T Marchi-Werle, L Palmer, NA Hunt, TE Sarath, G Heng-Moss, T AF Prochaska, T. J. Donze-Reiner, T. Marchi-Werle, L. Palmer, N. A. Hunt, T. E. Sarath, G. Heng-Moss, T. TI Transcriptional responses of tolerant and susceptible soybeans to soybean aphid (Aphis glycines Matsumura) herbivory SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Glycine max; Soybean; Aphis glycines; Soybean aphid; Plant resistance; Tolerance ID PLANT ARABIDOPSIS-THALIANA; SINGLE DOMINANT GENE; KUNITZ TRYPSIN-INHIBITOR; ABIOTIC STRESS TOLERANCE; LEUCINE-RICH REPEAT; RUSSIAN WHEAT APHID; HEMIPTERA APHIDIDAE; DEFENSE RESPONSES; ASCORBATE PEROXIDASE; MICROARRAY ANALYSIS AB The soybean aphid, Aphis glycines Matsumura, was introduced in 2000 to North America and has become one of the most significant pests to soybean, Glycine max (L.) Merrill, production. Possible solutions to this problem are the use of resistant plants and the understanding of the genes involved in plant resistance. In this study, we sought to better understand the genes involved in the tolerance response of soybean plants to the soybean aphid, utilizing tolerant (KS4202) and susceptible (K-03-4686) plants. Studies were conducted under greenhouse conditions. Leaf samples of both tolerant and susceptible plants were collected at day 5 and day 15 after infestation and analyzed by sequencing-by-synthesis on an Illumina GA II X instrument. In the tolerant genotype, 3 and 36 genes were found to be differentially expressed in the infested plants compared to the control treatments at day 5 and day 15, respectively. A similar comparison in the susceptible genotype revealed 0 and 11 genes to be differentially expressed at day 5 and day 15, respectively. Predominately, genes related to plant defense, such as WRKY transcription factors, peroxidases, and cytochrome p450s, were up-regulated in the tolerant genotype 15 days post-infestation by aphids. In contrast, none of these genes were similarly up-regulated in the susceptible plants, suggesting that consistent elevation of defense responses is important to plant tolerance. However, significant genotypic differences in global gene expression were also found when transcriptomes from control uninfested plants were compared at both day 5 and 15. qPCR validation of select genes confirmed our RNA-seq data. These comparisons indicate that potentially broader regulation of transcriptomes also contributes to the tolerance response and provides data that the tolerant genotype (KS4202) could be useful in soybean breeding programs trying to minimize production losses accruing from soybean aphid feeding. C1 [Prochaska, T. J.; Donze-Reiner, T.; Marchi-Werle, L.; Heng-Moss, T.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Palmer, N. A.; Sarath, G.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA, ARS, Lincoln, NE 68583 USA. [Hunt, T. E.] Univ Nebraska Lincoln, NEREC Haskell Agr Lab, Dept Entomol, Concord, NE 68728 USA. RP Heng-Moss, T (reprint author), Univ Nebraska, Dept Entomol, 103 Entomology Hall, Lincoln, NE 68583 USA. EM thengmoss2@unl.edu FU University of Nebraska Agricultural Experiment Station [NEB-28-097, NEB-41-034]; Nebraska Soybeans Board; North Central Soybean Research Program FX We acknowledge Rachael Sitz, Kyle Koch, Mitchell Stamm, Ruth Miller, and David Orr for technical assistance; Dr. Yuannan Xia for RNA extraction and sequencing; Dr. Jean-Jack Reithoven for data interpretation; and William T. Schapaugh, Jr., for providing the soybean germplasm for these studies. This research was supported in part by the University of Nebraska Agricultural Experiment Station Projects NEB-28-097 and NEB-41-034, the Nebraska Soybeans Board, and the North Central Soybean Research Program. The U.S. Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. NR 118 TC 2 Z9 2 U1 5 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD AUG PY 2015 VL 9 IS 4 BP 347 EP 359 DI 10.1007/s11829-015-9371-2 PG 13 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA CN1JR UT WOS:000358176300003 ER PT J AU Hadlocon, LJS Zhao, LY Wyslouzil, BE Zhu, HP AF Hadlocon, Lara Jane S. Zhao, Lingying Wyslouzil, Barbara E. Zhu, Heping TI Semi-mechanistic modelling of ammonia absorption in an acid spray wet scrubber based on mass balance SO BIOSYSTEMS ENGINEERING LA English DT Article DE Acid spray scrubber; Ammonia; Modelling; Overall mass transfer coefficient; Gas absorption ID TRANSFER COEFFICIENTS; SOLUTE CONCENTRATION; REMOVAL EFFICIENCY; PACKED COLUMNS; TOWER; PERFORMANCE; OPERATIONS; SO2 AB A model to describe reactive absorption of ammonia (NH3) in an acid spray scrubber was developed as a function of the combined overall mass transfer coefficient K(y)a(v). An experimental study of NH3 absorption using 1% dilute sulphuric acid was carried out under different operating conditions. An empirical correlation for K(y)a(v) with respect to droplet Sauter mean diameter, liquid flow rate, and inlet NH3 concentration was developed with an R-2 = 97.12%. Air velocity positively correlated with K(y)a(v) at 30 ppmv, but did not exhibit an effect at higher concentrations (165-300 ppmv), while liquid flow rate showed the greatest effect on K(y)a(v). The K(y)a(v) correlation was incorporated in the performance model to construct a semi-mechanistic model with high prediction accuracy (R-2 = 97.60%, MSE = 0, RMSE = 0.03, MAPE = 5.24%). This generalised performance model can be used to predict NH3 removal efficiencies of our optimised acid spray scrubber under various operating conditions at animal facilities. (C) 2015 IAgrE. Published by Elsevier Ltd. All rights reserved. C1 [Hadlocon, Lara Jane S.; Zhao, Lingying] Ohio State Univ, Dept Food Agr & Biol Engn, Columbus, OH 43210 USA. [Wyslouzil, Barbara E.] Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA. [Zhu, Heping] ARS, USDA, Wooster, OH 44691 USA. RP Zhao, LY (reprint author), Ohio State Univ, Dept Food Agr & Biol Engn, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM hadlocon.1@osu.edu; zhao.119@osu.edu; wyslouzil.1@osu.edu; heping.zhu@ars.usda.gov RI Wyslouzil, Barbara/G-8219-2012; OI Wyslouzil, Barbara/0000-0001-9763-5990 FU National Research Initiative Competitive Grant from the USDA Cooperative State Research, Education, and Extension Service Air Quality Program [2008-55112-1876] FX This project was supported by National Research Initiative Competitive Grant No. 2008-55112-1876 from the USDA Cooperative State Research, Education, and Extension Service Air Quality Program. The authors would also like to thank the help and contributions of Dr. Roderick Manuzon, Barry Nudd of USDA-ARS, Carl Cooper, Jared Felder, and Maxwell Pfenning. NR 26 TC 1 Z9 2 U1 3 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 EI 1537-5129 J9 BIOSYST ENG JI Biosyst. Eng. PD AUG PY 2015 VL 136 BP 14 EP 24 DI 10.1016/j.biosystemseng.2015.05.002 PG 11 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA CN4GT UT WOS:000358389300003 ER PT J AU Lam, TI Stanker, LH Lee, K Jin, RS Cheng, LW AF Lam, Tina I. Stanker, Larry H. Lee, Kwangkook Jin, Rongsheng Cheng, Luisa W. TI Translocation of botulinum neurotoxin serotype A and associated proteins across the intestinal epithelia SO CELLULAR MICROBIOLOGY LA English DT Article ID INCREASE TOXIN ABSORPTION; CLOSTRIDIUM-BOTULINUM; HA PROTEINS; E-CADHERIN; PROGENITOR TOXINS; COMPLEX; TRANSCYTOSIS; BARRIER; BINDING; CELLS AB Botulinum neurotoxins (BoNTs) are some of the most poisonous natural toxins. Botulinum neurotoxins associate with neurotoxin-associated proteins (NAPs) forming large complexes that are protected from the harsh environment of the gastrointestinal tract. However, it is still unclear how BoNT complexes as large as 900kDa traverse the epithelial barrier and what role NAPs play in toxin translocation. In this study, we examined the transit of BoNT serotype A (BoNT/A) holotoxin, complex and recombinantly purified NAP complex through cultured and polarized Caco-2 cells and, for the first time, in the small mouse intestine. Botulinum neurotoxin serotype A and NAPs in the toxin complex were detectable inside intestinal cells beginning at 2h post intoxication. Appearance of the BoNT/A holotoxin signal was slower, with detection starting at 4-6h. This indicated that the holotoxin alone was sufficient for entry but the presence of NAPs enhanced the rate of entry. Botulinum neurotoxin serotype A detection peaked at approximately 6 and 8h for complex and holotoxin, respectively, and thereafter began to disperse with some toxin remaining in the epithelia after 24h. Purified HA complexes alone were also internalized and followed a similar time course to that of BoNT/A complex internalization. However, recombinant HA complexes did not enhance BoNT/A holotoxin entry in the absence of a physical link with BoNT/A. We propose a model for BoNT/A toxin complex translocation whereby toxin complex entry is facilitated by NAPs in a receptor-mediated mechanism. Understanding the intestinal uptake of BoNT complexes will aid the development of new measures to prevent or treat oral intoxications. C1 [Lam, Tina I.; Stanker, Larry H.; Cheng, Luisa W.] USDA ARS, Foodborne Toxin Detect & Prevent Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Lee, Kwangkook; Jin, Rongsheng] Univ Calif Irvine, Sch Med, Physiol & Biophys, Irvine, CA 92697 USA. RP Cheng, LW (reprint author), USDA ARS, Foodborne Toxin Detect & Prevent Unit, Western Reg Res Ctr, Albany, CA 94710 USA. EM luisa.cheng@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service [NP108, CRIS 5325-42000-048-00D]; National Institute of Allergy and Infectious Diseases Service [U54 AI065359, RO1A1091823]; IAA [40768] FX The authors would like to acknowledge Drs. Kirkwood Land, Xiaohua He and Wallace Yokoyama for their helpful comments; Wanless Hatcher, Zeke Martinez and Jacqueline Miller for their help with animal care and handling. This work was funded by the United States Department of Agriculture, Agricultural Research Service, National Program Project NP108, CRIS 5325-42000-048-00D. L.W.C. and R.J. were also funded by the National Institute of Allergy and Infectious Diseases Service Grant U54 AI065359 and RO1A1091823, respectively. L.H.S. was also funded by Interagency Agreement IAA#40768. NR 40 TC 5 Z9 5 U1 2 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-5814 EI 1462-5822 J9 CELL MICROBIOL JI Cell Microbiol. PD AUG PY 2015 VL 17 IS 8 BP 1133 EP 1143 DI 10.1111/cmi.12424 PG 11 WC Cell Biology; Microbiology SC Cell Biology; Microbiology GA CN2LD UT WOS:000358251700006 PM 25640773 ER PT J AU Epanchin-Niell, RS Liebhold, AM AF Epanchin-Niell, Rebecca S. Liebhold, Andrew M. TI Benefits of invasion prevention: Effect of time lags, spread rates, and damage persistence SO ECOLOGICAL ECONOMICS LA English DT Article DE Biological invasion; Economic impact; Damages; Gypsy moth (Lymantria dispar); Hemlock woolly adelgid (Adelges tsugae); Emerald ash borer (Agrilus planipennis) ID BIOLOGICAL INVASIONS; SPECIES DISTRIBUTION; ECONOMIC-IMPACTS; RISK-ASSESSMENT; NORTH-AMERICA; MANAGEMENT; INVADERS; POPULATIONS; MODELS; COSTS AB Quantifying economic damages caused by invasive species is crucial for cost-benefit analyses of biosecurity measures. Most studies focus on short-term damage estimates, but evaluating exclusion or prevention measures requires estimates of total anticipated damages from the time of establishment onward. The magnitude of such damages critically depends on the timing of damages relative to a species arrival because costs are discounted back to the time of establishment. Using theoretical simulations, we illustrate how (ceteris paribus) total long-term damages, and hence the benefits of prevention efforts, are greater for species that a) have short lags between introduction and spread or between arrival at a location and initiation of damages, b) cause larger, short-lived damages (as opposed to smaller, persistent damages), and c) spread faster or earlier. We empirically estimate total long-term discounted impacts for three forest pests currently invading North America gypsy moth (Lymantria dispar), hemlock woolly adelgid (Adelges tsugae), and etherald ash borer (Agrilus planipennis) - and discuss how damage persistence, lags between introduction and spread, and spread rates affect damages. Many temporal characteristics can be predicted for new invaders and should be considered in species risk analyses and economic evaluations of quarantine and eradication programs. (c) 2015 Elsevier B.V. All rights reserved. C1 [Epanchin-Niell, Rebecca S.] Resources Future Inc, Washington, DC 20036 USA. [Liebhold, Andrew M.] US Forest Serv, No Res Stn, Morgantown, WV USA. RP Epanchin-Niell, RS (reprint author), Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA. EM epanchin-niell@rff.org; aliebhold@fs.fed.us NR 52 TC 6 Z9 6 U1 15 U2 70 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 EI 1873-6106 J9 ECOL ECON JI Ecol. Econ. PD AUG PY 2015 VL 116 BP 146 EP 153 DI 10.1016/j.ecolecon.2015.04.014 PG 8 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA CM5UI UT WOS:000357754100014 ER PT J AU Reynnells, R Callahan, MT Handy, ET Roberts, C Felton, G Ingram, DT Millner, PD Sharma, M AF Reynnells, Russell Callahan, Mary Theresa Handy, Eric T. Roberts, Cheryl Felton, Gary Ingram, David T. Millner, Patricia D. Sharma, Manan TI Evaluation of Two Immunomagnetic Separation Techniques for the Detection and Recovery of Escherichia coli O157:H7 from Finished Composts SO FOOD ANALYTICAL METHODS LA English DT Article DE Immunomagnetic; Compost; E. coli O157:H7; Real time PCR; Soil amendments ID BOVINE; MANURE; SALMONELLA; O157-H7 AB Two rapid immunomagnetic separation (IMS) protocols were evaluated to recover inoculated Escherichia coli O157:H7 (1-2 log colony-forming unit (CFU)/g) from 30 different commercial, finished compost samples. Both protocols detected E. coli O157:H7 in compost samples; PCR techniques required the removal of inhibitors to reduce possibility of a false negative result. C1 [Reynnells, Russell; Callahan, Mary Theresa; Handy, Eric T.; Roberts, Cheryl; Millner, Patricia D.; Sharma, Manan] ARS, USDA, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Area Res Ctr, Beltsville, MD 20705 USA. [Felton, Gary] Univ Maryland, Environm Sci & Technol Dept, Anim Sci Agr Engn 1433, College Pk, MD 20742 USA. [Ingram, David T.] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. RP Sharma, M (reprint author), ARS, USDA, Environm Microbial & Food Safety Lab, Henry A Wallace Beltsville Area Res Ctr, Bldg 201,Rm 103,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM rreynn555@aol.com; callahan.maryt@gmail.com; eric.handy@ars.usda.gov; cheryl.roberts@ars.usda.gov; gfelton@umd.edu; david.ingram@fda.hhs.gov; pat.millner@ars.usda.gov; manan.sharma@ars.usda.gov FU Center for Produce Safety grant; USDA-ARS "Microbial Ecology & Safety of Fresh on-Farm Organically Grown Produce" project FX Funding was provided by the Center for Produce Safety grant "Validation of testing methods for the detection and quantification of Escherichia coli O157:H7, Salmonella spp., fecal coliforms and nonpathogenic Escherichia coli in compost" and the USDA-ARS "Microbial Ecology & Safety of Fresh on-Farm Organically Grown Produce" project. NR 13 TC 0 Z9 0 U1 2 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1936-9751 EI 1936-976X J9 FOOD ANAL METHOD JI Food Anal. Meth. PD AUG PY 2015 VL 8 IS 7 BP 1812 EP 1814 DI 10.1007/s12161-014-0068-4 PG 3 WC Food Science & Technology SC Food Science & Technology GA CN3LC UT WOS:000358326700022 ER PT J AU Pham, K Pal, R Qu, Y Liu, X Yu, H Shiao, SL Wang, XQ Smith, EO Cui, XJ Rodney, GG Cheng, NH AF Pham, Khanh Pal, Rituraj Qu, Ying Liu, Xi Yu, Han Shiao, Stephen L. Wang, Xinquan Smith, E. O'Brian Cui, Xiaojiang Rodney, George G. Cheng, Ninghui TI Nuclear glutaredoxin 3 is critical for protection against oxidative stress-induced cell death SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE Oxidative stress; Cell death; Redox homeostasis; Glutaredoxin; Free radicals ID IRON-SULFUR CLUSTER; NF-KAPPA-B; SACCHAROMYCES-CEREVISIAE; MONOTHIOL GLUTAREDOXINS; S-GLUTATHIONYLATION; CARDIAC-HYPERTROPHY; PROTEIN-KINASE; CARDIOMYOCYTE CONTRACTILITY; INTERACTING PROTEIN; EPITHELIAL-CELLS AB Mammalian glutaredoxin 3 (Grx3) has been shown to be critical in maintaining redox homeostasis and regulating cell survival pathways in cancer cells. However, the regulation of Grx3 is not fully understood. In the present study, we investigate the subcellular localization of Grx3 under normal growth and oxidative stress conditions. Both fluorescence imaging of Grx3-RFP fusion and Western blot analysis of cellular fractionation indicate that Grx3 is predominantly localized in the cytoplasm under normal growth conditions, whereas under oxidizing conditions, Grx3 is translocated into and accumulated in the nucleus. Grx3 nuclear accumulation was reversible in a redox-dependent fashion. Further analysis indicates that neither the N-terminal Trx-like domain nor the two catalytic cysteine residues in the active CGFS motif of Grx3 are involved in its nuclear translocation. Decreased levels of Grx3 render cells susceptible to cellular oxidative stress, whereas overexpression of nuclear-targeted Grx3 is sufficient to suppress cells' sensitivity to oxidant treatments and reduce reactive oxygen species production. These findings provide novel insights into the regulation of Grx3, which is crucial for cell survival against environmental insults. (C) 2015 Elsevier Inc. All rights reserved, C1 [Pham, Khanh; Yu, Han; Smith, E. O'Brian; Cheng, Ninghui] USDA ARS, Childrens Nutr Res Ctr, Dept Phys, Houston, TX 77030 USA. [Pal, Rituraj; Rodney, George G.] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA. [Qu, Ying; Cui, Xiaojiang] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Dept Surg, Los Angeles, CA 90048 USA. [Qu, Ying; Cui, Xiaojiang] Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Dept Obstet & Gynecol, Los Angeles, CA 90048 USA. [Liu, Xi; Wang, Xinquan] Tsinghua Univ, Sch Life Sci, Ctr Struct Biol, Minist Educ,Key Lab Prot Sci, Beijing 100084, Peoples R China. [Shiao, Stephen L.] Cedars Sinai Med Ctr, Radiat Oncol & Biochem Sci, Los Angeles, CA 90048 USA. RP Cui, XJ (reprint author), Cedars Sinai Med Ctr, Samuel Oschin Comprehens Canc Inst, Dept Surg, Los Angeles, CA 90048 USA. EM Xiaojiang.Cui@cshs.org; ncheng@bcm.edu FU U.S. Department of Agriculture/Agricultural Research Service [6250-51000-054]; National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [R01 AR061370]; National Institutes of Health [CA151610]; Avon Foundation [02-2014-063]; David Salomon Translational Breast Cancer Research Fund; Fashion Footwear Charitable Foundation of New York, Inc.; Margie and Robert E. Petersen Foundation FX This work is supported by the U.S. Department of Agriculture/Agricultural Research Service under Cooperation Agreement 6250-51000-054 (N.H.C.), Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award R01 AR061370 to G.G.R and by National Institutes of Health (CA151610), the Avon Foundation (02-2014-063), David Salomon Translational Breast Cancer Research Fund, and the Fashion Footwear Charitable Foundation of New York, Inc. and the Margie and Robert E. Petersen Foundation to Y.Q. and X.C. NR 62 TC 2 Z9 3 U1 1 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 EI 1873-4596 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD AUG PY 2015 VL 85 BP 197 EP 206 DI 10.1016/j.freeradbiomed.2015.05.003 PG 10 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA CN1RO UT WOS:000358198000019 PM 25975981 ER PT J AU Van Meulder, F Ratman, D Van Coppernolle, S Borloo, J Li, RW Chiers, K Van den Broeck, W De Bosscher, K Claerebout, E Geldhof, P AF Van Meulder, F. Ratman, D. Van Coppernolle, S. Borloo, J. Li, R. W. Chiers, K. Van den Broeck, W. De Bosscher, K. Claerebout, E. Geldhof, P. TI Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE Cattle; Cooperia oncophora; Vaccine; Immune response ID NEMATODE TELADORSAGIA-CIRCUMCINCTA; OSTERTAGIA-OSTERTAGI; GASTROINTESTINAL NEMATODES; ANTHELMINTIC RESISTANCE; GENETICALLY RESISTANT; SECRETED PROTEIN; NK CELLS; INFECTION; CATTLE; SHEEP AB Recently we reported the successful vaccination of calves against Cooperia oncophora with a double domain activation-associated secreted protein, purified from the excretory-secretory material of adult stage parasites. In an attempt to elucidate the immune mechanisms involved in protection, the humoral and cell-mediated immune responses following vaccination and infection were compared with non-vaccinated control animals. Antigen-specific IgG1, IgG2 and IgA levels were significantly increased in sera of vaccinated animals post vaccination, whereas no effect was observed for IgM. Antigen-specific intestinal IgG1 levels were significantly increased in the vaccinated animals, whereas no differences were observed for antigen-specific IgA, IgM and IgG2 levels. Upon re-stimulation in vitro with the vaccine antigen, a significant proliferation of both alpha beta- and gamma delta-T cells, and B cells, collected from mesenteric lymph nodes, was only observed in vaccinated animals. RNA-seq analysis of intestinal tissue yielded a list of 67 genes that were differentially expressed in vaccinated animals following challenge infection, amongst which were several cell adhesion molecules, lectins and glycosyl transferases. A correlation analysis between all immunological and parasitological parameters indicated that intestinal anti-double domain activation-associated secreted protein IgG1 levels correlated negatively with cumulative faecal egg counts and positively with the proportion of L4s and L5s. The proportion of immature stages was also positively correlated with the proliferation of alpha beta T cells. Worm length was negatively correlated with the transcript levels of several lectins and cell adhesion molecules. Overall, the results indicate that intramuscular administration of the vaccine resulted in an immune memory response particularly characterised by increased antigen-specific IgG1 levels in the intestinal mucosa. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. C1 [Van Meulder, F.; Van Coppernolle, S.; Borloo, J.; Claerebout, E.; Geldhof, P.] Univ Ghent, Fac Vet Med, Dept Virol Parasitol & Immunol, B-9820 Merelbeke, Belgium. [Li, R. W.] USDA ARS, Bovine Funct Genom Lab, Anim & Nat Resources Inst, Beltsville, MD USA. [Chiers, K.] Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Avian Dis, B-9820 Merelbeke, Belgium. [Van den Broeck, W.] Univ Ghent, Fac Vet Med, Dept Morphol, B-9820 Merelbeke, Belgium. [Ratman, D.; De Bosscher, K.] Univ Ghent VIB, VIB Dept Med Prot Res, Nucl Receptor Lab, Receptor Res Labs, Ghent, Belgium. RP Geldhof, P (reprint author), Univ Ghent, Fac Vet Med, Parasitol Lab, Salisburylaan 133, B-9820 Merelbeke, Belgium. EM peter.geldhof@ugent.be FU Agency for Innovation by Science and Technology (IWT Vlaanderen), Belgium; European Union 7th Framework Program (PARAVAC project) FX This work was supported by the Agency for Innovation by Science and Technology (IWT Vlaanderen), Belgium and the European Union 7th Framework Program (PARAVAC project). NR 43 TC 2 Z9 2 U1 3 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 EI 1879-0135 J9 INT J PARASITOL JI Int. J. Parasit. PD AUG PY 2015 VL 45 IS 9-10 BP 637 EP 646 DI 10.1016/j.ijpara.2015.03.007 PG 10 WC Parasitology SC Parasitology GA CN5IC UT WOS:000358462000008 PM 25937360 ER PT J AU Rothschild, J Rosentrater, KA Onwulata, C Singh, M Menutti, L Jambazian, P Omary, MB AF Rothschild, Jeffrey Rosentrater, Kurt A. Onwulata, Charles Singh, Mukti Menutti, Lorena Jambazian, Pera Omary, Maria B. TI Influence of quinoa roasting on sensory and physicochemical properties of allergen-free, gluten-free cakes SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Cake; coeliac; gluten-free; quinoa; roasting ID CHENOPODIUM-QUINOA; FUNCTIONAL-PROPERTIES; ATRIPLEX-HORTENSIS; CELIAC PATIENTS; PARTICLE-SIZE; XANTHAN GUM; IN-VITRO; QUALITY; FLOUR; ANTIOXIDANT AB The objectives of this study were to determine pasting properties of non-roasted (NR) and roasted quinoa (RQ) and to investigate the effect of RQ on consumer acceptance and physicochemical properties of an allergen-free, gluten-free cake formulation. Quinoa seeds were roasted at 177 degrees C for 15 (R15), 30 (R30) and 45min (R45), and flours were analysed for pasting properties. Five cakes including a commercial chocolate cake (CCC) and cakes made with NR and RQ flours were evaluated for preference by fifty panelists. Quality parameters included colour, water activity, moisture content, firmness, weight and height. Peak and final viscosity increased with roasting time. The NR cake had the highest sensory scores for appearance, colour and texture. On flavour and overall acceptability, CCC was the highest. Regarding quality data, CCC, NR and R15 cakes had similar L* values, while CCC had the lowest a*, b*, a(w), moisture content and firmness values. C1 [Rothschild, Jeffrey; Menutti, Lorena; Jambazian, Pera; Omary, Maria B.] Calif State Univ Los Angeles, Sch Kinesiol & Nutr Sci, Los Angeles, CA 90032 USA. [Rosentrater, Kurt A.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [Onwulata, Charles] USDA, Washington, DC 20250 USA. [Singh, Mukti] USDA ARS NCAUR, Peoria, IL 61604 USA. RP Omary, MB (reprint author), Calif State Univ Los Angeles, Sch Kinesiol & Nutr Sci, 5151 State Univ Dr, Los Angeles, CA 90032 USA. EM momary@calstatela.edu OI Rosentrater, Kurt/0000-0003-0131-7037 FU Southern California Section of the Institute of Food Technologists (SCI-FTS); California State University Agricultural Research Institute (ARI) FX The authors wish to thank the Southern California Section of the Institute of Food Technologists (SCI-FTS) and the California State University Agricultural Research Institute (ARI) for partial funding of this project. In addition, the authors would like to thank Audrey Thomas-Gahring, Tawana Simons and Eric Tilman (USDA-ERRC, Wyndmoor, PA), for their outstanding support with this research, and Food Science and Technology students at Cal Poly Pomona including Julie Nguyen and Olivia Zawadi. NR 72 TC 3 Z9 3 U1 9 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD AUG PY 2015 VL 50 IS 8 BP 1873 EP 1881 DI 10.1111/ijfs.12837 PG 9 WC Food Science & Technology SC Food Science & Technology GA CN2MR UT WOS:000358256100023 ER PT J AU Kiszonas, AM Fuerst, EP Luthria, D Morris, CF AF Kiszonas, Alecia M. Fuerst, E. Patrick Luthria, Devanand Morris, Craig F. TI Arabinoxylan content and characterisation throughout the bread-baking process SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Arabinose: xylose ratio; arabinoxylans; bread; water-extractable arabinoxylans; wheat ID WATER-SOLUBLE PENTOSANS; WHEAT-FLOUR PENTOSANS; END-USE QUALITY; NONSTARCH POLYSACCHARIDES; LIQUID-CHROMATOGRAPHY; FUNCTIONAL-PROPERTIES; PHYSICAL-PROPERTIES; DOUGH; FRACTIONS; GLUTEN AB End-use quality of wheat (Triticum aestivum L.) is influenced in a variety of ways by nonstarch polysaccharides, especially arabinoxylan (AX). The objective of this study was to track total and water-extractable AX (TAX and WEAX, respectively) throughout the bread-baking process, using wholemeal and refined flour. The TAX and WEAX content and the ratio of arabinose: xylose were assessed in flour, mixed dough, proofed dough and the bread loaf, which was separated into crumb, upper crust and bottom crust. Changes in TAX during the baking process differed between the refined flour and wholemeal samples, suggesting a change in the TAX availability which we ascribe to molecular interactions and heat treatment. WEAX content dramatically decreased during baking, suggesting that oxidative cross-linkages rendered it unextractable. Higher levels of WEAX and lower levels of arabinose substitution were correlated with higher loaf volumes for refined flour among the hard wheat varieties. Having a better understanding of the importance of both WEAX content and arabinose substitution allows for directed breeding efforts towards improved hard wheat varieties for optimum bread baking. C1 [Kiszonas, Alecia M.; Morris, Craig F.] Washington State Univ, USDA ARS Western Wheat Qual Lab, Pullman, WA 99164 USA. [Fuerst, E. Patrick] Washington State Univ, Dept Crop & Soil Sci, Coll Agr Human & Nat Resource Sci, Pullman, WA 99164 USA. [Fuerst, E. Patrick] USDA ARS, Western Wheat Qual Lab, Washington, DC 20250 USA. [Luthria, Devanand] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. RP Morris, CF (reprint author), Washington State Univ, USDA ARS Western Wheat Qual Lab, E 202 Food Qual Bldg, Pullman, WA 99164 USA. EM morrisc@wsu.edu FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2009-02347] FX The work was supported by the Agriculture and Food Research Initiative Grant no. 2009-02347 from the USDA National Institute of Food and Agriculture. NR 29 TC 2 Z9 2 U1 4 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD AUG PY 2015 VL 50 IS 8 BP 1911 EP 1921 DI 10.1111/ijfs.12829 PG 11 WC Food Science & Technology SC Food Science & Technology GA CN2MR UT WOS:000358256100027 ER PT J AU Staicu, LC Ackerson, CJ Cornelis, P Ye, L Berendsen, RL Hunter, WJ Noblitt, SD Henry, CS Cappa, JJ Montenieri, RL Wong, AO Musilova, L Sura-de Jong, M van Hullebusch, ED Lens, PNL Reynolds, RJB Pilon-Smits, EAH AF Staicu, L. C. Ackerson, C. J. Cornelis, P. Ye, L. Berendsen, R. L. Hunter, W. J. Noblitt, S. D. Henry, C. S. Cappa, J. J. Montenieri, R. L. Wong, A. O. Musilova, L. Sura-de Jong, M. van Hullebusch, E. D. Lens, P. N. L. Reynolds, R. J. B. Pilon-Smits, E. A. H. TI Pseudomonas moraviensis subsp stanleyae, a bacterial endophyte of hyperaccumulator Stanleya pinnata, is capable of efficient selenite reduction to elemental selenium under aerobic conditions SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE aerobic selenite reduction; elemental selenium nanoparticles; microchip capillary electrophoresis; multi-locus sequence analysis; Pseudomonas moraviensis; Stanleya pinnata ID CONTACT CONDUCTIVITY DETECTION; MICROCHIP ELECTROPHORESIS; SULFUR ACCUMULATION; NANOPARTICLES; SOIL; CONTAMINATION; ENVIRONMENT; RESISTANCE; METALS; GROWTH AB AimsTo identify bacteria with high selenium tolerance and reduction capacity for bioremediation of wastewater and nanoselenium particle production. Methods and ResultsA bacterial endophyte was isolated from the selenium hyperaccumulator Stanleya pinnata (Brassicaceae) growing on seleniferous soils in Colorado, USA. Based on fatty acid methyl ester analysis and multi-locus sequence analysis (MLSA) using 16S rRNA, gyrB, rpoB and rpoD genes, the isolate was identified as a subspecies of Pseudomonas moraviensis (973% nucleotide identity) and named P.moraviensis stanleyae. The isolate exhibited extreme tolerance to SeO32- (up to 120mmoll(-1)) and SeO42- (>150mmoll(-1)). Selenium oxyanion removal from growth medium was measured by microchip capillary electrophoresis (detection limit 95nmoll(-1) for SeO32- and 13nmoll(-1) for SeO42-). Within 48h, P.moraviensis stanleyae aerobically reduced SeO32- to red Se(0) from 10mmoll(-1) to below the detection limit (removal rate 027mmolh(-1) at 30 degrees C); anaerobic SeO32- removal was slower. No SeO42- removal was observed. Pseudomonas moraviensis stanleyae stimulated the growth of crop species Brassica juncea by 70% with no significant effect on Se accumulation. ConclusionsPseudomonas moraviensis stanleyae can tolerate extreme levels of selenate and selenite and can deplete high levels of selenite under aerobic and anaerobic conditions. Significance and Impact of the StudyPseudomonas moraviensis subsp. stanleyae may be useful for stimulating plant growth and for the treatment of Se-laden wastewater. C1 [Staicu, L. C.; Reynolds, R. J. B.; Pilon-Smits, E. A. H.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Staicu, L. C.; Cappa, J. J.; Lens, P. N. L.] UNESCO IHE Inst Water Educ, Delft, Netherlands. [Staicu, L. C.; van Hullebusch, E. D.] Univ Paris Est, Lab Geomat & Environm, UPEM, Marne La Vallee 2, France. [Ackerson, C. J.; Noblitt, S. D.; Henry, C. S.; Wong, A. O.] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA. [Cornelis, P.; Ye, L.] Vrije Univ, VIB Dept Struct Biol, Dept Bioengn Sci, Microbiol Res Grp, Brussels, Belgium. [Berendsen, R. L.] Univ Utrecht, Fac Sci, Dept Biol, Plant Microbe Interact, Utrecht, Netherlands. [Hunter, W. J.; Montenieri, R. L.] USDA ARS, Ft Collins, CO 80522 USA. [Musilova, L.; Sura-de Jong, M.] Prague Inst Chem Technol, Dept Biochem & Microbiol, CR-16628 Prague, Czech Republic. RP Pilon-Smits, EAH (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM epsmits@lamar.colostate.edu RI Musilova, Lucie/J-4522-2012; Berendsen, Roeland/I-1594-2016; OI Musilova, Lucie/0000-0002-8244-8006; van Hullebusch, Eric D./0000-0002-1519-1925 FU Fulbright Commission; European Commission through Erasmus Mundus Joint Doctorate Programme ETeCoS3 (Environmental Technologies for Contaminated Solids, Soils and Sediments) [2010-0009]; Czech Ministry of Education, Youth and Sports through KONTAKT [LH12087] FX We thank the Fulbright Commission for providing Lucian Staicu with financial support during his research stage at Colorado State University. A special thanks to the European Commission for providing financial support through the Erasmus Mundus Joint Doctorate Programme ETeCoS3 (Environmental Technologies for Contaminated Solids, Soils and Sediments) under the grant agreement FPA no 2010-0009. We also thank the Czech Ministry of Education, Youth and Sports for the financial support through the project KONTAKT LH12087. We are indebted to Dr. J. Euzeby for correcting the etymology of the isolate. NR 45 TC 6 Z9 6 U1 4 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD AUG PY 2015 VL 119 IS 2 BP 400 EP 410 DI 10.1111/jam.12842 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CN2KI UT WOS:000358249400009 PM 25968181 ER PT J AU McGarvey, JA Han, R Connell, JH Stanker, LH Hnasko, R AF McGarvey, J. A. Han, R. Connell, J. H. Stanker, L. H. Hnasko, R. TI Bacterial populations on the surfaces of organic and conventionally grown almond drupes SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE agriculture; almond; environmental; microbial phylogenetics; microbial structure ID PHYLLOSPHERE; DIVERSITY; COMMUNITY; MICROBIOLOGY; PARAMETERS; ECOLOGY; IMPACT AB AimsTo compare the bacterial populations on organically and conventionally grown almond drupes before and after hull split. Methods and ResultsWe constructed 16S rRNA gene libraries, containing approx. 3000 sequences each, from the bacteria from organically and conventionally grown drupes before and after hull split. We observed that before hull split both conventionally and organically grown drupes were colonized by relatively few types of bacteria that were mostly common phyllosphere-associated Proteobacteria. However, the organically grown drupes contained significantly more Alphaproteobacteria and the conventionally grown drupes contained significantly more Gammaproteobacteria. The conventionally grown drupes also contained significantly more sequences associated with the phylum Actinobacteria. After hull split, we observed a significant increase in bacterial diversity, with many newly appearing sequences that were not normally associated with the phyllosphere. ConclusionsOrganic and conventional growing methodologies influence the types of bacteria on almond drupes and hull split results in a burst of microbial diversification. Significance and Impact of the StudyProduction of organic produce is increasing due to consumer preferences, but it was unknown how this methodology affects the bacterial populations on almond drupes. This is the first study to compare the bacterial populations of organically and conventionally grown almond drupes. C1 [McGarvey, J. A.; Stanker, L. H.; Hnasko, R.] ARS, Foodborne Toxin Detect & Protect Res Unit, USDA, Albany, CA USA. [Han, R.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Connell, J. H.] Univ Calif Cooperat Extens, Oroville, CA USA. RP McGarvey, JA (reprint author), 800 Buchanan St, Albany, CA 94133 USA. EM Jeffery.mcgarvey@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service, National Program, Food Safety [108] FX This work was funded by the U.S. Department of Agriculture, Agricultural Research Service, National Program 108, Food Safety. NR 42 TC 1 Z9 1 U1 2 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD AUG PY 2015 VL 119 IS 2 BP 529 EP 538 DI 10.1111/jam.12850 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CN2KI UT WOS:000358249400021 PM 25974141 ER PT J AU Onwulata, C Thomas-Gahring, A Oduro-Yeboah, C White, AK Hotchkiss, AT AF Onwulata, Charles Thomas-Gahring, Audrey Oduro-Yeboah, Charlotte White, Andre K. Hotchkiss, Arland T. TI Effects of Uniquely Processed Cowpea and Plantain Flours on Wheat Bread Properties SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID GAS-LIQUID-CHROMATOGRAPHY; VIGNA-UNGUICULATA FLOUR; GLYCEMIC INDEX; FUNCTIONAL-PROPERTIES; RHIZOPUS MICROSPORUS; AVAILABLE GLUCOSE; HUMAN FOOD; FERMENTATION; QUALITY; PERFORMANCE AB The effect of incorporating uniquely processed whole-seed cowpeas or plantain flours at 10 or 20g/100g in all-purpose wheat flour on paste viscosity and bread-baking properties in a model bread was determined. Blanching plantains in hot water (100C) for 2min increased final viscosity, reduced rapidly available glucose (RAG) from 8.5 to 4.5g glucose/mg, and increased bread loaf size. Whole-seed cowpeas containing the hulls processed by soaking in water (CPS), reduced raffinose significantly (P<0.05), and -galactosidase enzyme (CPE) treatment eliminated both raffinose and stachyose completely. CPS decreased RAG values from 8.5 to 2.8g glucose/mg. Incorporating cowpea flours into model wheat breads increased loaf size. CPE flours reduced bread loaf size and increased internal browning. These flours can be incorporated into all-purpose wheat flour bread up to 20g/100g with improved physical properties, and possibly better glycemic responses. Practical ApplicationsPlantain is an emerging source of slowly digestible starch, and cowpeas are rich in proteins and micronutrients that could help to combat metabolic syndrome. Unique processes that convert whole-seed cowpeas and plantains into dried flours will enhance commercial utilization of these tropical produce. Converting peak season cowpea and plantain into shelf-stable food powders will provide long-term storage, eliminate postharvest losses, add economic value, and enhance food safety and security in the tropics. C1 [Onwulata, Charles; Thomas-Gahring, Audrey; White, Andre K.; Hotchkiss, Arland T.] ARS, CEEPR, Dairy & Funct Foods Res, Eastern Reg Res Ctr,USDA, Wyndmoor, PA 19038 USA. [Oduro-Yeboah, Charlotte] CSIR, Food Res Inst, Accra, Ghana. RP Onwulata, C (reprint author), ARS, CEEPR, Dairy & Funct Foods Res, Eastern Reg Res Ctr,USDA, Wyndmoor, PA 19038 USA. EM charles.onwulata@osec.usda.gov FU [58-1935-9-174F] FX The contribution of United States Department of Agriculture-Agricultural Research Service, Office of International Research Programs and the United States Agency for International Development is appreciated. Grant Agreement 58-1935-9-174F. NR 37 TC 0 Z9 0 U1 4 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD AUG PY 2015 VL 39 IS 4 BP 413 EP 422 DI 10.1111/jfpp.12246 PG 10 WC Food Science & Technology SC Food Science & Technology GA CN4ZR UT WOS:000358439600010 ER PT J AU O'Donnell, V Holinka, LG Krug, PW Gladue, DP Carlson, J Sanford, B Alfano, M Kramer, E Lu, ZQ Arzt, J Reese, B Carrillo, C Risatti, GR Borca, MV AF O'Donnell, Vivian Holinka, Lauren G. Krug, Peter W. Gladue, Douglas P. Carlson, Jolene Sanford, Brenton Alfano, Marialexia Kramer, Edward Lu, Zhiqiang Arzt, Jonathan Reese, Bo Carrillo, Consuelo Risatti, Guillermo R. Borca, Manuel V. TI African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge SO JOURNAL OF VIROLOGY LA English DT Article ID VIRAL VIRULENCE; MACROPHAGES; GROWTH; PIGS AB African swine fever virus (ASFV) is the etiological agent of an often lethal disease of domestic pigs. Disease control strategies have been hampered by the unavailability of vaccines against ASFV. Since its introduction in the Republic of Georgia, a highly virulent virus, ASFV Georgia 2007 (ASFV-G), has caused an epizootic that spread rapidly into Eastern European countries. Currently no vaccines are available or under development to control ASFV-G. In the past, genetically modified ASFVs harboring deletions of virulence-associated genes have proven attenuated in swine, inducing protective immunity against challenge with homologous parental viruses. Deletion of the gene 9GL (open reading frame [ORF] B119L) in highly virulent ASFV Malawi-Lil-20/1 produced an attenuated phenotype even when administered to pigs at 10(6) 50% hemadsorption doses (HAD(50)). Here we report the construction of a genetically modified ASFV-G strain (ASFV-G-Delta 9GLv) harboring a deletion of the 9GL (B119L) gene. Like Malawi-Lil-20/1-Delta 9GL, ASFV-G-Delta 9GL showed limited replication in primary swine macrophages. However, intramuscular inoculation of swine with 10(4) HAD(50) of ASFV-G-Delta 9GL produced a virulent phenotype that, unlike Malawi-Lil-20/1-Delta 9GL, induced a lethal disease in swine like parental ASFV-G. Interestingly, lower doses (10(2) to 10(3) HAD(50)) of ASFV-G-Delta 9GL did not induce a virulent phenotype in swine and when challenged protected pigs against disease. A dose of 10(2) HAD(50) of ASFV-G-Delta 9GLv conferred partial protection when pigs were challenged at either 21 or 28 days postinfection (dpi). An ASFV-G-Delta 9GL HAD(50) of 10(3) conferred partial and complete protection at 21 and 28 dpi, respectively. The information provided here adds to our recent report on the first attempts toward experimental vaccines against ASFV-G. IMPORTANCE The main problem for controlling ASF is the lack of vaccines. Studies on ASFV virulence lead to the production of genetically modified attenuated viruses that induce protection in pigs but only against homologous virus challenges. Here we produced a recombinant ASFV lacking virulence-associated gene 9GL in an attempt to produce a vaccine against virulent ASFV-G, a highly virulent virus isolate detected in the Caucasus region in 2007 and now spreading though the Caucasus region and Eastern Europe. Deletion of 9GL, unlike with other ASFV isolates, did not attenuate completely ASFV-G. However, when delivered once at low dosages, recombinant ASFV-G-Delta 9GL induces protection in swine against parental ASFV-G. The protection against ASFV-G is highly effective after 28 days postvaccination, whereas at 21 days postvaccination, animals survived the lethal challenge but showed signs of ASF. Here we report the design and development of an experimental vaccine that induces protection against virulent ASFV-G. C1 [O'Donnell, Vivian; Holinka, Lauren G.; Krug, Peter W.; Gladue, Douglas P.; Carlson, Jolene; Sanford, Brenton; Alfano, Marialexia; Arzt, Jonathan; Borca, Manuel V.] ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [O'Donnell, Vivian; Gladue, Douglas P.; Risatti, Guillermo R.] Univ Connecticut, Dept Pathobiol & Vet Sci, CAHNR, Storrs, CT USA. [Reese, Bo] Univ Connecticut, Ctr Genome Innovat, Storrs, CT USA. [Kramer, Edward; Lu, Zhiqiang] US Dept Homeland Secur, Plum Isl Anim Dis Ctr, Greenport, NY USA. [Carrillo, Consuelo] USDA, APHIS, Plum Isl Anim Dis Ctr, Greenport, NY USA. RP Borca, MV (reprint author), ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM manuel.borca@ars.usda.gov OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178; Arzt, Jonathan/0000-0002-7517-7893 FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-11-X-00077, HSHQPM-12-X-00005]; ARS/USDA-University of Connecticut [SCA 58-1940-1-190] FX This project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under award no. HSHQDC-11-X-00077 and HSHQPM-12-X-00005. We thank ARS/USDA-University of Connecticut SCA 58-1940-1-190 for partially supporting this work. NR 23 TC 6 Z9 6 U1 0 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD AUG PY 2015 VL 89 IS 16 BP 8556 EP 8566 DI 10.1128/JVI.00969-15 PG 11 WC Virology SC Virology GA CN2UT UT WOS:000358278200038 PM 26063424 ER PT J AU Stanton, RA Thompson, FR Kesler, DC AF Stanton, Richard A. Thompson, Frank R., III Kesler, Dylan C. TI Site occupancy of brown-headed nuthatches varies with habitat restoration and range-limit context SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Arkansas; brown-headed nuthatch; dispersal; habitat associations; habitat restoration; occupancy models; prescribed fire; range extension; Sitta pusilla ID R-PACKAGE; MODELS; BIRD; WOODPECKERS; POPULATION; DISPERSAL; ABUNDANCE; SELECTION; BEHAVIOR; SCALE AB Knowledge about species' responses to habitat restoration can inform subsequent management and reintroduction planning. We used repeated call-response surveys to study brown-headed nuthatch (Sitta pusilla) patch occupancy at the current limits of its apparently expanding range in an area with active habitat restoration. We fit a probit occupancy model that accounted for spatial autocorrelation using restricted spatial regression. Nuthatch occupancy was related to patch-level vegetation structure and range-extension context, i.e., latitude, but not prescribed fire history. Latitude and percent tree stocking had a negative relationship with occupancy (coefficients and 95% credible intervals: -1.07 [CI: -1.63, -0.67] and -0.63 [CI: -0.97, -0.350]). The density of recently killed and well-decayed snags had positive associations with occupancy (coefficients and 95% credible intervals: 0.57 [CI: 0.17, 1.16] and 0.37 [CI: 0.05, 0.72]). Neither grassy herbaceous cover nor percent of stocking in pine were associated with occupancy. We found that restoration efforts created suitable stand structure for brown-headed nuthatches, but many restored sites in the range-extension zone appeared to be vacant. Occupied habitats in the range-extension zone had fewer snags, less frequent fire, and more shrub cover than occupied sites where the species was established. Release from conspecific competition may have permitted nuthatches in the range-extension zone to exploit habitats that would otherwise have been marginal. Alternatively, nuthatches may be restricted to such sites although there are more suitable sites tens of kilometers away. Experimental translocations and reintroductions could determine how habitat structure and nuthatch density affect the quality of restored sites in the range-extension zone and enable those sites to achieve their biodiversity potential. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Stanton, Richard A.; Kesler, Dylan C.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. RP Stanton, RA (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. EM richardastanton@ufl.edu OI Stanton, Richard/0000-0001-9187-2186 NR 51 TC 0 Z9 0 U1 4 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2015 VL 79 IS 6 BP 917 EP 926 DI 10.1002/jwmg.903 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CN1JX UT WOS:000358177000007 ER PT J AU Augustine, DJ Derner, JD AF Augustine, David J. Derner, Justin D. TI Patch-burn grazing management, vegetation heterogeneity, and avian responses in a semi-arid grassland SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE fire-grazing interactions; grasshopper sparrow; horned lark; lark bunting; McCown's longspur; mountain plover; prescribed fire; pyric herbivory; shortgrass steppe; western meadowlark ID SHORTGRASS STEPPE; NEST SURVIVAL; PYRIC-HERBIVORY; SPATIAL HETEROGENEITY; COMMUNITY STRUCTURE; BIRD COMMUNITIES; GREAT-PLAINS; PRAIRIE DOG; FIRE; CONSERVATION AB Anthropogenic changes to disturbance regimes in grasslands, and associated homogenization of vegetation structure, have been implicated as factors contributing to declines in populations of grassland birds in North America. We examined the influence of patch-burn grazing management, which employs spatiotemporal interactions between fire and livestock grazing guided by historical disturbance patterns, on vegetation structure and bird abundance in shortgrass steppe in northeastern Colorado, USA. All study pastures were grazed by cattle at moderate stocking rates from May to October each year. In the patch-burn treatment, we burned 25% of each pasture in autumn (Oct or Nov) each year during 2007-2010; control pastures were not burned. Patch-burn grazing management increased vegetation heterogeneity by generating short (<4cm), sparse vegetation on recent burns. Although cattle selectively grazed recent burns, this did not alter vegetation structure in unburned portions of patch-burned pastures relative to controls. Of the 6 grassland bird species we examined, mountain plovers (Charadrius montanus) occurred exclusively on recent burns, whereas grasshopper sparrows (Ammodramus savannarum) occurred exclusively in grassland not burned for 3 years. Two species (lark bunting [Calamospiza melanocorys] and western meadowlark [Sturnella neglecta]) were 2-3 times less abundant on recent burns compared to controls, whereas densities of horned larks (Eremophila alpestris) and McCown's longspur (Rhynchophanes mccownii) were unaffected by burning. Lark bunting, western meadowlark, and grasshopper sparrow densities varied substantially among years. In the years when they were abundant, all 3 species increased in density across the time-since-burning gradient. Consistent with this pattern, patch-burn grazing management reduced the abundance of all 3 species at the whole-pasture scale. We found no evidence that unburned patches within the patch-burned pastures differed from unburned pastures in terms of the abundance of any bird species. Patch-burn grazing management was an effective strategy to create breeding habitat for mountain plovers. However, our findings suggest that in the shortgrass steppe, additional strategies that generate taller, more dense vegetation than occurs under moderate cattle grazing need to be considered in combination with patch-burn grazing management to sustain breeding habitat for the full suite of native grassland birds. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Augustine, David J.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. [Derner, Justin D.] ARS, Rangeland Resources Res Unit, USDA, Cheyenne, WY 82009 USA. RP Augustine, DJ (reprint author), ARS, Rangeland Resources Res Unit, USDA, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM David.Augustine@ars.usda.gov NR 47 TC 3 Z9 3 U1 4 U2 74 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2015 VL 79 IS 6 BP 927 EP 936 DI 10.1002/jwmg.909 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CN1JX UT WOS:000358177000008 ER PT J AU Wu, JX Siegel, RB Loffland, HL Tingley, MW Stock, SL Roberts, KN Keane, JJ Medley, JR Bridgman, R Stermer, C AF Wu, Joanna X. Siegel, Rodney B. Loffland, Helen L. Tingley, Morgan W. Stock, Sarah L. Roberts, Kevin N. Keane, John J. Medley, Joseph R. Bridgman, Roy Stermer, Chris TI Diversity of great gray owl nest sites and nesting habitats in California SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE California; forest management; great gray owl; nest tree characteristics; nesting habitat; Strix nebulosa ID YOSEMITE-NATIONAL-PARK; STRIX-NEBULOSA; SIERRA-NEVADA; SPOTTED OWLS AB The great gray owl (Strix nebulosa) is listed by the state of California as endangered, with a population estimate of fewer than 300 individuals in the state. Nest-site availability has been suggested as a limiting factor for population growth in California, but information on nest types and nesting habitat has been based on a small number of nests that may not fully represent the variety of conditions used by the species. We collated all known nesting records in the Sierra Nevada mountains of California since 1973 (n=56) and then visited 47 of the nest sites to characterize habitat and compare them with paired reference sites. Great gray owls used a diversity of trees (8 species) and nest types. Although great gray owls in California are considered conifer-forest specialists, 30% of nests were in oak trees and 21% were below 1,000m, which loosely corresponds to the lower conifer-zone limit. Across all elevations and tree species, degree of deterioration was the most important factor differentiating nest trees from paired reference trees at the same meadow, with nest trees being significantly more decayed. Nest trees (mean dbh=100.5 +/- SD 30.3cm) were also significantly larger than reference trees. Canopy cover within 50m of nest trees ((x) over bar =85.1 +/- 16.4%) was significantly greater at nest sites than at reference sites. At higher elevations, most nests were within 250m of a meadow edge, but at lower elevations, 31% of nests were >750m from the closest meadow. Based on these findings, we suggest that managers trying to promote great gray owl nesting maintain 4 or more large (100-cm dbh) snags per hectare in dense forests, especially near meadows. We also recommend increasing great gray owl survey effort in habitats and areas that may have been inadequately surveyed in the past. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Wu, Joanna X.; Siegel, Rodney B.; Loffland, Helen L.] Inst Bird Populat, Point Reyes Stn, CA 94956 USA. [Tingley, Morgan W.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Stock, Sarah L.] Yosemite Natl Pk, El Portal, CA 95318 USA. [Roberts, Kevin N.] Sierra Pacific Ind, Camino, CA 95709 USA. [Keane, John J.; Medley, Joseph R.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Medley, Joseph R.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Bridgman, Roy] Tahoe Natl Forest, Amer River Ranger Dist, Foresthill, CA 95631 USA. [Stermer, Chris] Calif Dept Fish & Wildlife, Sacramento, CA 95811 USA. RP Wu, JX (reprint author), Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. EM jwu@birdpop.org OI Tingley, Morgan/0000-0002-1477-2218 FU California Department of Fish and Wildlife; Yosemite Conservancy FX We thank the California Department of Fish and Wildlife and the Yosemite Conservancy for funding this project. Sierra Pacific Industries and Southern California Edison kindly granted us access to nests on their lands. We thank our field crew, S. Hein, M. Lerow, M. Poling, K. Strohm, B. Dudek, S. Eyes, T. Ely, N. Ludington, R. Carlton, and R. Byrnes for their careful data collection. M. Reno, S. Byrd, P. Krueger, K. Sorini, A. Otto, T. Lowe, J. and C. Winter, J. Cordes, J. Croteau, M. Flores, and C. Battistone shared valuable information regarding the location of nest trees and/or took us to nests. Two anonymous reviewers and the editors made suggestions that substantially improved this paper. This study was completed by The Institute for Bird Populations' Sierra Nevada Bird Observatory and is Contribution No. 488 of The Institute for Bird Populations. NR 34 TC 1 Z9 1 U1 4 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2015 VL 79 IS 6 BP 937 EP 947 DI 10.1002/jwmg.910 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CN1JX UT WOS:000358177000009 ER PT J AU Forcey, GM Thogmartin, WE Linz, GM Mckann, PC Crimmins, SM AF Forcey, Greg M. Thogmartin, Wayne E. Linz, George M. Mckann, Patrick C. Crimmins, Shawn M. TI Spatially explicit modeling of blackbird abundance in the Prairie Pothole Region SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE bayesian; blackbirds; climate; crop damage; hierarchical models; landscape; MCMC; North American Breeding Bird Survey; Prairie Pothole Region; relative abundance; weather ID BREEDING BIRD SURVEY; RED-WINGED BLACKBIRDS; LAND-COVER DATA; UNITED-STATES; HIERARCHICAL ANALYSIS; CERULEAN WARBLERS; HABITAT SELECTION; AVIAN ABUNDANCE; LANDSCAPE-SCALE; WETLAND BIRDS AB Knowledge of factors influencing animal abundance is important to wildlife biologists developing management plans. This is especially true for economically important species such as blackbirds (Icteridae), which cause more than $100 million in crop damages annually in the United States. Using data from the North American Breeding Bird Survey, the National Land Cover Dataset, and the National Climatic Data Center, we modeled effects of regional environmental variables on relative abundance of 3 blackbird species (red-winged blackbird, Agelaius phoeniceus; yellow-headed blackbird, Xanthocephalus xanthocephalus; common grackle, Quiscalus quiscula) in the Prairie Pothole Region of the central United States. We evaluated landscape covariates at 3 logarithmically related spatial scales (1,000ha, 10,000ha, and 100,000ha) and modeled weather variables at the 100,000-ha scale. We constructed models a priori using information from published habitat associations. We fit models with WinBUGS using Markov chain Monte Carlo techniques. Both landscape and weather variables contributed strongly to predicting blackbird relative abundance (95% credibility interval did not overlap 0). Variables with the strongest associations with blackbird relative abundance were the percentage of wetland area and precipitation amount from the year before bird surveys were conducted. The influence of spatial scale appeared smallmodels with the same variables expressed at different scales were often in the best model subset. This large-scale study elucidated regional effects of weather and landscape variables, suggesting that management strategies aimed at reducing damages caused by these species should consider the broader landscape, including weather effects, because such factors may outweigh the influence of localized conditions or site-specific management actions. The regional species distributional models we developed for blackbirds provide a tool for understanding these broader landscape effects and guiding wildlife management practices to areas that are optimally beneficial. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Forcey, Greg M.] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58105 USA. [Thogmartin, Wayne E.; Mckann, Patrick C.; Crimmins, Shawn M.] US Geol Survey, Upper Midwest Environm Sci Ctr, La Crosse, WI 54603 USA. [Linz, George M.] USDA, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. RP Forcey, GM (reprint author), Normandeau Associates, 102 NE 10th Ave, Gainesville, FL 32601 USA. EM research@gregforcey.com RI Thogmartin, Wayne/A-4461-2008 OI Thogmartin, Wayne/0000-0002-2384-4279 FU National Wildlife Research Center, a unit within the United States Department of Agriculture, Animal Plant and Health Inspection Service, Wildlife Services (WS); Department of Biological Sciences and the Graduate School at North Dakota State University; [WS-NWRCQA-1039] FX We thank W. J. Bleier, M. E. Biondini, G. K. Clambey, and G. L. Nuechterlein for their contributions and advice on this study. D. Anderson, D. Granfors, R. Johnson, N. Niemuth, and J. Sauer provided suggestions on the data analyses for this project. We also thank E. O'Neal for providing ArcGIS plugins to simplify the spatial analyses of weather and land use data. M. R. Sones provided copy-editing assistance on the tables and supplementary material. K. Hill edited the manuscript for content and flow. We thank B. R. Gray and 2 anonymous reviewers for their comments on an earlier version of this manuscript, improving it immensely. Thousands of volunteers annually conduct breeding bird survey counts, and we are grateful for their efforts. This project was funded by the National Wildlife Research Center, a unit within the United States Department of Agriculture, Animal Plant and Health Inspection Service, Wildlife Services (WS). G. M. Linz was the study director for this project (WS-NWRCQA-1039). Financial support was also provided by the Department of Biological Sciences and the Graduate School at North Dakota State University. Any use of trade, product, or firm names are for descriptive purposes only and do not imply endorsement by the U.S. Government. NR 73 TC 1 Z9 1 U1 2 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2015 VL 79 IS 6 BP 1022 EP 1033 DI 10.1002/jwmg.912 PG 12 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CN1JX UT WOS:000358177000017 ER PT J AU Bendele, KG Guerrero, FD Miller, RJ Li, AY Barrero, RA Moolhuijzen, PM Black, M McCooke, JK Meyer, J Hill, CA Bellgard, MI AF Bendele, Kylie G. Guerrero, Felix D. Miller, Robert J. Li, Andrew Y. Barrero, Roberto A. Moolhuijzen, Paula M. Black, Michael McCooke, John K. Meyer, Jason Hill, Catherine A. Bellgard, Matthew I. TI Acetylcholinesterase 1 in populations of organophosphate-resistant North American strains of the cattle tick, Rhipicephalus microplus (Acari: Ixodidae) SO PARASITOLOGY RESEARCH LA English DT Article DE Rhipicephalus microplus; Protein coding sequence; Acaricide resistance; Organophosphate; Allele; Acetylcholinesterase; AChE ID BOOPHILUS-MICROPLUS; MEXICAN STRAINS; INSECTICIDE RESISTANCE; MEMBRANE-PROTEINS; GENOME PROJECT; CDNA; PREDICTION; MUTATIONS; SEQUENCE; DATABASE AB Rhipicephalus microplus, the cattle fever tick, is a global economic problem to the cattle industry due to direct infestation of cattle and pathogens transmitted during feeding. Cattle fever tick outbreaks continue to occur along the Mexico-US border even though the tick has been eradicated from the USA. The organophosphate (OP) coumaphos targets acetylcholinesterase (AChE) and is the approved acaricide for eradicating cattle fever tick outbreaks. There is evidence for coumaphos resistance developing in cattle ticks in Mexico, and OP-resistant R. microplus ticks were discovered in outbreak populations of Texas in 2005. The molecular basis of coumaphos resistance is not known, and our study was established to gather further information on whether AChE1 is involved in the resistance mechanism. We also sought information on allele diversity in tick populations with different levels of coumaphos resistance. The overarching project goal was to define OP resistance-associated gene mutations such that a DNA-based diagnostic assay could be developed to assist the management of resistance. Three different AChE transcripts have been reported in R. microplus, and supporting genomic and transcriptomic data are available at CattleTickBase. Here, we report the complete R. microplus AChE1 gene ascertained by sequencing a bacterial artificial chromosome clone containing the entire coding region and the flanking 5' and 3' regions. We also report AChE1 sequences of larval ticks from R. microplus strains having different sensitivities to OP. To accomplish this, we sequenced a 669-bp region of the AChE1 gene corresponding to a 223 amino acid region of exon 2 to assess alleles in seven strains of R. microplus with varying OP resistance phenotypes. We identified 72 AChE1 sequence variants, 2 of which are strongly associated with OP-resistant phenotypes. Esterase-like sequences from the R. microplus transcriptome RmiTr Version 1.0 were compared to the available sequence databases to identify other transcripts with similarity to AChE1. C1 [Bendele, Kylie G.; Guerrero, Felix D.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Miller, Robert J.] USDA ARS, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. [Li, Andrew Y.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Barrero, Roberto A.; Moolhuijzen, Paula M.; Black, Michael; McCooke, John K.; Bellgard, Matthew I.] Murdoch Univ, Ctr Comparat Genom, Murdoch, WA 6150, Australia. [Meyer, Jason; Hill, Catherine A.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Meyer, Jason] Monsanto Co, Div Biotechnol, Chesterfield, MO 63017 USA. RP Guerrero, FD (reprint author), USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM Kylie.Bendele@ars.usda.gov; felix.guerrero@ars.usda.gov; Robert.Miller@ars.usda.gov; Andrew.Li@ars.usda.gov; rbarrero@ccg.murdoch.edu.au; pmoolhuijzen@ccg.murdoch.edu.au; mblack@ccg.murdoch.edu.au; jmccooke@ccg.murdoch.edu.au; hessianfly@yahoo.com; hillca@purdue.edu; mbellgard@ccg.murdoch.edu.au RI Black, Michael/H-7645-2016 FU Purdue University; US Department of Agriculture-Agricultural Research Service CRIS [6205-32000-026-00D] FX The authors wish to thank Dr. Phillip San Miguel of the Purdue Genomics Core Facility (Purdue University, West Lafayette, IN) for excellent technical support with the BAC sequencing, assembly and automated annotation and Purdue University for support of this part of the research through institutional funds. We also thank Drs. Kevin B. Temeyer and Roger Ivan Rodriguez-Vivas for their helpful review of the manuscript. KB carried out the DNA, PCR, and bioinformatic protocols and wrote the first draft of the manuscript, FG conceived of and directed the project and participated in writing/editing the manuscript, RM and AL directed and conducted the tick strain collections and selections and participated in editing the manuscript, RB, PM, MB, JKM, and MIB participated in the bioinformatic analysis of the sequenced BAC and assembling the Sanger-sequenced contigs using the R. microplus genome sequencing dataset, and JM and CH assisted in bioinformatic analysis of the BAC clone and editing the manuscript. The authors acknowledge funding support from the US Department of Agriculture-Agricultural Research Service CRIS Project No. 6205-32000-026-00D. USDA is an equal opportunity employer. NR 41 TC 0 Z9 0 U1 1 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD AUG PY 2015 VL 114 IS 8 BP 3027 EP 3040 DI 10.1007/s00436-015-4505-0 PG 14 WC Parasitology SC Parasitology GA CN4OV UT WOS:000358410600025 PM 25952704 ER PT J AU Walters, C AF Walters, Christina TI Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss SO PLANTA LA English DT Review DE Cell shrinkage; Configurational entropy; Desiccation; Desiccation tolerance; Glass formation; Plasmolysis; Temperature; Threshold ID MEDICAGO-TRUNCATULA SEEDS; MOISTURE-CONTENT LIMIT; DESICCATION TOLERANCE; MOLECULAR MOBILITY; HERMETIC STORAGE; LATE MATURATION; EMBRYONIC AXES; LETTUCE SEEDS; LONGEVITY; SURVIVAL AB Main conclusion Discrete categories of seed physiology can be explained through a unified concept of the structural and molecular mobility responses within cells to drying. Tolerance of desiccation is typically described by a threshold or low water content limit to survival. This convention provides fairly good distinction between orthodox and recalcitrant seeds, which show thresholds of less than about 0.07 and greater than about 0.2 g H2O g DW-1, respectively. Threshold water contents, however, are not direct measures of the intensity of water stress tolerated by seeds, nor are they measures of cell response to water stress. More direct criteria, that accommodate both spatial and temporal effects of water loss, are required to explain variation of desiccation tolerance and longevity in seeds from diverse genetic backgrounds and growth conditions. This essay presents the argument that changes in cellular volume directly quantify primary responses to desiccating stress in a context that also links damage, as cellular constituents compress, and protection, as compressed molecules form stabilizing structure. During desiccation, fluid cytoplasm solidifies, and the newly formed spatial relationships among molecules determine whether and how long viability is maintained. The diversity of seed behaviors suggests complexity and opportunity to discover molecules and mechanisms that regulate survival and perception of time in cells that lack metabolic function. C1 USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Walters, C (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80521 USA. EM christina.walters@ars.usda.gov NR 77 TC 10 Z9 10 U1 14 U2 54 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD AUG PY 2015 VL 242 IS 2 BP 397 EP 406 DI 10.1007/s00425-015-2312-6 PG 10 WC Plant Sciences SC Plant Sciences GA CN1GZ UT WOS:000358168000005 PM 25985842 ER PT J AU Goncalves, VN Carvalho, CR Johann, S Mendes, G Alves, TMA Zani, CL Junior, PAS Murta, SMF Romanha, AJ Cantrell, CL Rosa, CA Rosa, LH AF Goncalves, Vivian N. Carvalho, Camila R. Johann, Susana Mendes, Graziele Alves, Tania M. A. Zani, Carlos L. Junior, Policarpo A. S. Murta, Silvane M. F. Romanha, Alvaro J. Cantrell, Charles L. Rosa, Carlos A. Rosa, Luiz H. TI Antibacterial, antifungal and antiprotozoal activities of fungal communities present in different substrates from Antarctica SO POLAR BIOLOGY LA English DT Article DE Antarctica; Bioprospecting; Extremophiles; Fungi ID PURPUREOCILLIUM-LILACINUM; PAECILOTOXIN PRODUCTION; DIVERSITY; MACROALGAE; GEOMYCES; DISEASE; DESV.; SOILS AB Antarctica is a pristine and extreme environment that represents a unique opportunity for taxonomic, ecological and biotechnological studies of the microorganisms. In the present work, the fungal communities of rhizosphere soil of Deschampsia antarctica, soil, ornithogenic soil, marine and lake sediments in the Antarctic Peninsula were characterized as well as their capability to produce bioactive compounds. One hundred fungal isolates were recovered and identified by molecular analysis in 35 different taxa of 20 genera. Pseudogymnoascus sp. 1 and 3, Penicillium sp., Peniophora sp. and Mortierella alpina were the most frequent taxa identified. All isolates were cultured to produce ethanolic extracts, which were assayed against different target organisms to detect antimicrobial (against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Candida krusei, Cladosporium sphaerospermum and Paracoccidioides brasiliensis), cytotoxic (against breast MCF-7 and renal TK-10 human tumoral cells) and antiprotozoal (against Leishmania amazonensis and Trypanosoma cruzi) activities. Among the three human pathogenic fungal species, 20 extracts showed moderate to high and selective antifungal activity against P. brasiliensis. The extract of Purpureocillium lilacinum displayed high trypanocidal, antifungal and antibacterial activities, but with moderate toxicity over normal cells. Proton nuclear magnetic resonance (H-1 NMR) spectral analysis indicated the presence of compounds containing a highly functionalized aromatic ring system. Our results suggest that the Antarctic ecosystems represent an interesting habitat for the isolation and characterization of fungal taxa capable to producing bioactive compounds. The fungus P. lilacinum showed strong trypanocidal and antimicrobial activities with moderate toxicity over normal cells, which might be used as scaffold for the development of new drugs. C1 [Goncalves, Vivian N.; Carvalho, Camila R.; Johann, Susana; Mendes, Graziele; Rosa, Carlos A.; Rosa, Luiz H.] Univ Fed Minas Gerais, Inst Ciencias Biolg, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. [Alves, Tania M. A.; Zani, Carlos L.; Junior, Policarpo A. S.; Murta, Silvane M. F.; Romanha, Alvaro J.] FIOCRUZ MG, Rene Rachou Res Ctr, Belo Horizonte, MG, Brazil. [Romanha, Alvaro J.] Univ Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, Florianopolis, SC, Brazil. [Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. RP Rosa, LH (reprint author), Univ Fed Minas Gerais, Inst Ciencias Biolg, Dept Microbiol, POB 486, BR-31270901 Belo Horizonte, MG, Brazil. EM lhrosa@icb.ufmg.br RI Zani, Carlos/A-9658-2008 OI Zani, Carlos/0000-0003-1859-177X FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [Processes PROANTAR 407230/2013-0]; INCT Criosfera [Universal 471721/2013-1]; Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [Process Universal 0050-13]; Financiadora de Estudos e Projetos [FINEP 2084/07]; Program for Technological Development of Tools for Health-PDTIS-FIOCRUZ FX We acknowledge the financial support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Processes PROANTAR 407230/2013-0, INCT Criosfera, Universal 471721/2013-1, Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Process Universal 0050-13 and the Financiadora de Estudos e Projetos (FINEP 2084/07), the Program for Technological Development of Tools for Health-PDTIS-FIOCRUZ. We thank the Brazilian Navy for the logistic support. Additionally, we thank the Dr. D.I. Shapiro-Ilan USDA-ARS, SAA for the manuscript review. NR 37 TC 2 Z9 2 U1 5 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0722-4060 EI 1432-2056 J9 POLAR BIOL JI Polar Biol. PD AUG PY 2015 VL 38 IS 8 BP 1143 EP 1152 DI 10.1007/s00300-015-1672-5 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CM9PQ UT WOS:000358041600004 ER PT J AU Olanrewaju, HA Purswell, JL Collier, SD Branton, SL AF Olanrewaju, H. A. Purswell, J. L. Collier, S. D. Branton, S. L. TI Effects of color temperatures (Kelvin) of LED bulbs on blood physiological variables of broilers grown to heavy weights SO POULTRY SCIENCE LA English DT Article DE light-emitting diode; acid-base balance; blood gases; broilers; well-being ID ACID-BASE-BALANCE; LIGHT-INTENSITY; SPECTRAL SENSITIVITY; MONOCHROMATIC LIGHT; CHICKENS; POTASSIUM; STRESS; PHOTORECEPTION; PERFORMANCE; DOMESTICUS AB Light-emitting diode (LED) lighting is being used in the poultry industry to reduce energy usage in broiler production facilities. However, limited data are available comparing efficacy of different spectral distribution of LED bulbs on blood physiological variables of broilers grown to heavy weights (>3 kg). The present study evaluated the effects of color temperature (Kelvin) of LED bulbs on blood physiological variables of heavy broilers in 2 trials with 4 replicates/trial. The study was a randomized complete block design. Four light treatments consisted of 3 LED light bulbs [2,700 K, (Warm-LED); 5,000 K, (Cool-LED-#1); 5,000 K, (Cool-LED-#2)] and incandescent light (ICD, standard) from 1 to 56 d age. A total of 960 1-day-old Ross x Ross 708 chicks (30 males/room 30 females/room) were equally and randomly distributed among 16 environmentally controlled rooms at 50% RH. Each of the 4 treatments was represented by 4 rooms. Feed and water were provided ad libitum. All treatment groups were provided the same diet. Venous blood samples were collected on d 21, 28, 42, and 56 for immediate analysis of selected physiological variables and plasma collection. In comparison with ICD, Cool-LED-#1 had greater (P < 0.05) effects on pH, partial pressure of CO2 (pCO(2)), partial pressure of O-2 (pO(2)), saturated O-2 (sO(2)), and K+. However, all these acid-base changes remained within the normal venous acid-base homeostasis and physiological ranges. In addition, no effect of treatments was observed on HCO3-, hematocrit (Hct), hemoglobin (Hb), Na+, Ca2+, Cl-, mean corpuscular hemoglobin concentration (McHc), osmolality, and anion gap. Moreover, blood glucose concentrations were not affected by treatments. This study shows that the 3 LED light bulbs evaluated in this study may be suitable for replacement of ICD light sources in commercial poultry facilities to reduce energy cost and optimize production efficiency without inducing physiological stress on broilers grown to heavy weights. C1 [Olanrewaju, H. A.; Purswell, J. L.; Collier, S. D.; Branton, S. L.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. RP Olanrewaju, HA (reprint author), ARS, USDA, Poultry Res Unit, POB 5367, Mississippi State, MS 39762 USA. EM Hammed.Olanrewaju@ars.usda.gov NR 45 TC 1 Z9 1 U1 2 U2 12 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2015 VL 94 IS 8 BP 1721 EP 1728 DI 10.3382/ps/pev139 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CN1NG UT WOS:000358186100001 PM 26047670 ER PT J AU Shanmugasundaram, R Kogut, MH Arsenault, RJ Swaggerty, CL Cole, K Reddish, JM Selvaraj, RK AF Shanmugasundaram, Revathi Kogut, Michael H. Arsenault, Ryan J. Swaggerty, Christina L. Cole, Kimberly Reddish, John M. Selvaraj, Ramesh K. TI Effect of Salmonella infection on cecal tonsil regulatory T cell properties in chickens SO POULTRY SCIENCE LA English DT Article DE Salmonella; Tregs; persistance; IL-10 ID ANTIVIRAL IMMUNE-RESPONSE; IN-VITRO; VACCINATION STRATEGIES; VIRAL-INFECTION; DISEASE; VIRUS; VIVO; COCCIDIOSIS; SUPPRESSION; CD4(+) AB Two studies were conducted to study regulatory T cell [Treg (CD4(+) CD25(+))] properties during the establishment of a persistent intestinal infection in broiler chickens. Four-day-old broiler chicks were orally gavaged with 5 x 10(6) CFU/mL Salmonella enteritidis (S. enteritidis) or sterile PBS (control). Samples were collected at 4, 7, 10, and 14 d postinfection. There was a significant (P < 0.05) increase in the number of CD4(+) CD25(+) cells by d 4 postinfection that increased steadily throughout the course of the 14-d infection, whereas the number of CD4(+)CD25(+) cells in the noninfected controls remained steady throughout the study. CD4(+)CD25(+) cells from cecal tonsils of S. enteritidis-infected birds had a higher (P < 0.05) IL-10 mRNA content than CD4(+)CD25(+) cells from the noninfected controls at all time-points studied. The amount of IL-2 mRNA content in the cecal tonsil CD4(+)CD25(-) cells from the infected birds did not differ (P > 0.05) when compared to that of noninfected control birds. At a lower effector/responder cell ratio of 0.25: 1, CD4(+)CD25(+) cells from cecal tonsils of Salmonella-infected birds suppressed T cell proliferation at d 7 and 14 post-S. enteritidis infection, while CD4(+)CD25(+) cells from non-infected control groups did not suppress T cell proliferation. In the second studu, 1-day-old chickens were orally gavaged with PBS (control) or 1.25 x 10(8) CFU/bird S. enteritidis. At 7 and 21 d post-Salmonella infection, CD25(+) cells collected from cecal tonsils of S. enteritidis-infected birds and restimulated in vitro with Salmonella antigen had higher (P < 0.05) IL-10 mRNA content compared to those in the control group. Spleen CD4(+)CD25(+), CD4(+), and CD8(+) cell percentage did not differ (P > 0.05) between the Salmonella-infected and control birds. In conclusion, a persistent intestinal S. enteritidis infection increased the Treg percentage, suppressive properties, and IL-10 mRNA amounts in the cecal tonsils of broiler birds. C1 [Shanmugasundaram, Revathi; Cole, Kimberly; Reddish, John M.; Selvaraj, Ramesh K.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA. [Kogut, Michael H.; Arsenault, Ryan J.; Swaggerty, Christina L.] USDA ARS, Plains Area, College Stn, TX 77845 USA. RP Selvaraj, RK (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA. EM selvaraj.7@osu.edu FU Ohio Agricultural Research and Development Center SEEDS grants FX The authors have no conflict of interest. This study was partially funded by Ohio Agricultural Research and Development Center SEEDS grants awarded to Ramesh Selvaraj. Mention of commercial products is for the sole purpose of providing specific information, not recommendation or endorsement by the USDA. NR 40 TC 3 Z9 3 U1 1 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2015 VL 94 IS 8 BP 1828 EP 1835 DI 10.3382/ps/pev161 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CN1NG UT WOS:000358186100014 PM 26049799 ER PT J AU Evans, JD Leigh, SA Purswell, JL Collier, SD Kim, EJ Boykin, DL Branton, SL AF Evans, J. D. Leigh, S. A. Purswell, J. L. Collier, S. D. Kim, E. J. Boykin, D. L. Branton, S. L. TI The impact of deposition site on vaccination efficiency of a live bacterial poultry vaccine SO POULTRY SCIENCE LA English DT Article DE poultry vaccine; live vaccine; spray vaccination; vaccine deposition; Mycoplasma gallisepticum; AviPro MG F ID MYCOPLASMA-GALLISEPTICUM; F-STRAIN; LAYER CHICKENS AB Vaccines are utilized within the poultry industry to minimize disease-associated losses and spray vaccination is a commonly utilized means for the mass application of poultry vaccines. During this process, vaccine-laden particles are deposited upon target areas (e.g., eyes, nares, and oral cavity) resulting in the direct internalization of the vaccine. However, particles are also deposited on nontarget areas such as the exterior of the subject and its surrounding environment. To better determine the fate of particles deposited upon nontarget areas and the impact of deposition site on the efficiency of vaccine application, a live bacterial poultry vaccine (AviPro((R)) MG F) was applied via spray using a spray cabinet with a slotted partition allowing for head-only, body-only, and whole-bird spray application. At 11 wk age, Hy-Line((R)) W-36 pullets (n = 280) were allocated equally among 7 treatments including: nonvaccinated controls, pullets spray-vaccinated at the manufacturer's recommended dose (1X) in a site-specific manner (head-only, body-only, and whole-bird), pullets spray-vaccinated at 5X the recommended level (body-only), pullets vaccinated by manual eye-drop application (1X), and pullets eye-drop vaccinated at a level approximating that achieved during the spray vaccination process (1/700X). At 6 to 7 wk postvaccination, vaccination efficiency was assessed via serological-based assays [serum plate agglutination (SPA) and ELISA] and the detection of vaccine-derived in vivo populations. Results indicate an additive contribution of the vaccine deposited on the body to the overall vaccination efficiency of this live bacterial live poultry vaccine. C1 [Evans, J. D.; Leigh, S. A.; Purswell, J. L.; Collier, S. D.; Kim, E. J.; Branton, S. L.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. [Boykin, D. L.] ARS, USDA, Mid South Area Off, Stoneville, MS 38776 USA. RP Evans, JD (reprint author), ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. EM jeff.evans@ars.usda.gov NR 16 TC 0 Z9 0 U1 1 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2015 VL 94 IS 8 BP 1849 EP 1852 DI 10.3382/ps/pev149 PG 4 WC Agriculture, Dairy & Animal Science SC Agriculture GA CN1NG UT WOS:000358186100017 PM 26049801 ER PT J AU Rabin, BM Heroux, NA Shukitt-Hale, B Carrihill-Knoll, KL Beck, Z Baxter, C AF Rabin, Bernard M. Heroux, Nicholas A. Shukitt-Hale, Barbara Carrihill-Knoll, Kirsty L. Beck, Zachary Baxter, Chelsea TI Lack of reliability in the disruption of cognitive performance following exposure to protons SO RADIATION AND ENVIRONMENTAL BIOPHYSICS LA English DT Article DE Protons; Behavior; Novel object recognition; Operant responding ID OBJECT RECOGNITION MEMORY; HEAVY-PARTICLE IRRADIATION; FE-56 PARTICLES; HIPPOCAMPAL NEUROGENESIS; SPACE RADIATION; BRAIN-DAMAGE; FE-56-PARTICLE RADIATION; PERIRHINAL CORTEX; OXIDATIVE STRESS; HZE PARTICLES AB A series of three replications were run to determine the reliability with which exposure to protons produces a disruption of cognitive performance, using a novel object recognition task and operant responding on an ascending fixed-ratio task. For the first two replications, rats were exposed to head-only exposures to 1000 MeV/n protons at the NASA Space Radiation Laboratory. For the third replication, subjects were given head-only or whole-body exposures to both 1000 and 150 MeV/n protons. The results were characterized by a lack of consistency in the effects of exposure to protons on the performance of these cognitive tasks, both within and between replications. The factors that might influence the lack of consistency and the implications for exploratory class missions are discussed. C1 [Rabin, Bernard M.; Heroux, Nicholas A.; Carrihill-Knoll, Kirsty L.; Beck, Zachary; Baxter, Chelsea] UMBC, Dept Psychol, Baltimore, MD 21250 USA. [Shukitt-Hale, Barbara] Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Rabin, BM (reprint author), UMBC, Dept Psychol, Baltimore, MD 21250 USA. EM rabin@umbc.edu OI Heroux, Nicholas/0000-0001-6242-0770 FU National Aeronautics and Space Administration [NNJ06HD93G, NNX08AM66G, NNX13AB73G] FX The research reported here was carried out under National Aeronautics and Space Administration Grants NNJ06HD93G, NNX08AM66G and NNX13AB73G. NR 56 TC 0 Z9 0 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0301-634X EI 1432-2099 J9 RADIAT ENVIRON BIOPH JI Radiat. Environ. Biophys. PD AUG PY 2015 VL 54 IS 3 BP 285 EP 295 DI 10.1007/s00411-015-0597-2 PG 11 WC Biology; Biophysics; Environmental Sciences; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Biophysics; Environmental Sciences & Ecology; Radiology, Nuclear Medicine & Medical Imaging GA CN3LU UT WOS:000358328800004 PM 25935209 ER PT J AU Chen, J Carrillo, Y Pendall, E Dijkstra, FA Evans, RD Morgan, JA Williams, DG AF Chen, Janet Carrillo, Yolima Pendall, Elise Dijkstra, Feike A. Evans, R. Dave Morgan, Jack A. Williams, David G. TI Soil Microbes Compete Strongly with Plants for Soil Inorganic and Amino Acid Nitrogen in a Semiarid Grassland Exposed to Elevated CO2 and Warming SO ECOSYSTEMS LA English DT Article DE amino acids; global change; grasslands; nitrogen uptake; C-13; N-15 ID ATMOSPHERIC CARBON-DIOXIDE; SEDGE KOBRESIA-MYOSUROIDES; ORGANIC-NITROGEN; EXTRACTION METHOD; FREEZE-THAW; ECOSYSTEM; FORMS; WATER; AVAILABILITY; LIMITATION AB Free amino acids (FAAs) in soil are an important N source for plants, and abundances are predicted to shift under altered atmospheric conditions such as elevated CO2. Composition, plant uptake capacity, and plant and microbial use of FAAs relative to inorganic N forms were investigated in a temperate semiarid grassland exposed to experimental warming and free-air CO2 enrichment. FAA uptake by two dominant grassland plants, Bouteloua gracilis and Artemesia frigida, was determined in hydroponic culture. B. gracilis and microbial N preferences were then investigated in experimental field plots using isotopically labeled FAA and inorganic N sources. Alanine and phenylalanine concentrations were the highest in the field, and B. gracilis and A. frigida rapidly consumed these FAAs in hydroponic experiments. However, B. gracilis assimilated little isotopically labeled alanine, ammonium and nitrate in the field. Rather, soil microbes immobilized the majority of all three N forms. Elevated CO2 and warming did not affect plant or microbial uptake. FAAs are not direct sources of N for B. gracilis, and soil microbes outcompete this grass for organic and inorganic N when N is at peak demand within temperate semiarid grasslands. C1 [Chen, Janet] Univ Wyoming, Dept Ecosyst Sci & Management, Laramie, WY 82071 USA. [Chen, Janet; Pendall, Elise; Williams, David G.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Carrillo, Yolima; Pendall, Elise] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Pendall, Elise; Williams, David G.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Dijkstra, Feike A.] Univ Sydney, Dept Environm Sci, Ctr Carbon Water & Food, Camden, NSW 2570, Australia. [Evans, R. Dave] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Morgan, Jack A.] Washington State Univ, WSU Stable Isotope Core Facil, Sch Biol Sci, Pullman, WA 99163 USA. RP Chen, J (reprint author), Univ Wyoming, Dept Ecosyst Sci & Management, Laramie, WY 82071 USA. EM janet.chen@unh.edu; y.carrillo@uws.edu.au FU US Department of Agriculture Agricultural Research Service Climate Change, Soils & Emissions Program; USDA-CSREES Soil Processes Program [2008-35107-18655]; US Department of Energy Office of Science (BER) [DE-SC0006973]; Western Regional Center of the National Institute for Climatic Change Research; National Science Foundation (DEB) [1021559] FX Special thanks to David Legg, David Perry, Dan Lecain, Chandelle MacDonald, Amanda Brennan, Dana Blumenthal, Justin Jones, Erik Hobbie, and Jane Zelikova for manuscript, laboratory, and field assistance. This material is based upon work supported by the US Department of Agriculture Agricultural Research Service Climate Change, Soils & Emissions Program, USDA-CSREES Soil Processes Program (#2008-35107-18655), US Department of Energy Office of Science (BER), through the Terrestrial Ecosystem Science program (#DE-SC0006973) and the Western Regional Center of the National Institute for Climatic Change Research, and by the National Science Foundation (DEB#1021559). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. NR 59 TC 3 Z9 3 U1 22 U2 70 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD AUG PY 2015 VL 18 IS 5 BP 867 EP 880 DI 10.1007/s10021-015-9868-7 PG 14 WC Ecology SC Environmental Sciences & Ecology GA CN0FI UT WOS:000358089100011 ER PT J AU Dobrowski, SZ Swanson, AK Abatzoglou, JT Holden, ZA Safford, HD Schwartz, MK Gavin, DG AF Dobrowski, Solomon Z. Swanson, Alan K. Abatzoglou, John T. Holden, Zachary A. Safford, Hugh D. Schwartz, Mike K. Gavin, Daniel G. TI Forest structure and species traits mediate projected recruitment declines in western US tree species SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Article DE Climate change; demographic niche differences; facilitation; tree regeneration; water balance; western USA ID CLIMATE-CHANGE; UNITED-STATES; DOUGLAS-FIR; VEGETATION DISTRIBUTION; SPATIAL VARIATION; ALPINE TREELINE; CONIFER FORESTS; SIERRA-NEVADA; NICHE; CALIFORNIA AB AimDetermine if differences in the climatic niche between conspecific adult and juvenile trees of the western Unites States vary by species traits and to assess if forest canopies moderate the sensitivity of juvenile trees to climatic variation. LocationThe western Unites States. MethodsUsing data from the USDA Forest Inventory and Analysis programme, we compare the distribution of conspecific adult and juvenile trees for 62 western US tree species. We relate demographic niche differences to species traits including shade and drought tolerance. We model recruitment under projected climate change using generalized linear mixed models, probabilistic uncertainty accounting, forest structural data and projected changes in the climatic water balance. ResultsOn average juveniles of western US tree species occupy a climatic subset of their conspecific adults. Demographic niche differences increase as species shade and drought tolerance increase and are greatest at climatic range margins, indicating the potential for range contractions. Models calibrated solely with climate data project recruitment declines for 2080 that are 47% larger on average than models that also account for forest structure. Main conclusionsClimate change-driven declines in recruitment in western US tree species may be partly offset by the moderating effect of forest canopies. The importance of this stabilizing process will depend on whether a given site is disturbed and the traits of resident species, including their ability to utilize sites that have buffered microclimates. Conversely, our results suggest that broad-scale disturbances which result in the loss of forest canopy will amplify the effects of climate change on tree recruitment. C1 [Dobrowski, Solomon Z.; Swanson, Alan K.] Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. [Abatzoglou, John T.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. [Holden, Zachary A.] US Forest Serv, USDA, Missoula, MT USA. [Safford, Hugh D.] US Forest Serv, USDA, Vallejo, CA USA. [Schwartz, Mike K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Gavin, Daniel G.] Univ Oregon, Dept Geog, Eugene, OR 97403 USA. RP Dobrowski, SZ (reprint author), Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. EM solomon.dobrowski@umontana.edu RI Gavin, Daniel/C-9214-2009; Schwartz, Michael/C-3184-2014; OI Gavin, Daniel/0000-0001-8743-3949; Schwartz, Michael/0000-0003-3521-3367; Abatzoglou, John/0000-0001-7599-9750 FU National Science Foundation [1145985]; Montana Institute on Ecosystems; NSF EPSCoR grant [EPS-1101342]; USDA Forest Service Rocky Mountain Research Station [JV11221635-201]; USFS Region 5 FX Support for this research was provided by the National Science Foundation (DEB; 1145985), the Montana Institute on Ecosystems (as supported by NSF EPSCoR grant EPS-1101342), the USDA Forest Service Rocky Mountain Research Station (JV11221635-201), and the USFS Region 5. We are grateful to R. McCullough with the USFS Spatial Data Services for extracting climate data and providing FIA plot data. NR 57 TC 17 Z9 17 U1 4 U2 51 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X EI 1466-8238 J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD AUG PY 2015 VL 24 IS 8 BP 917 EP 927 DI 10.1111/geb.12302 PG 11 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA CM7FL UT WOS:000357855700005 ER PT J AU Cassone, BJ Redinbaugh, MG Dorrance, AE Michel, AP AF Cassone, Bryan J. Redinbaugh, Margaret G. Dorrance, Anne E. Michel, Andrew P. TI Shifts in Buchnera aphidicola density in soybean aphids (Aphis glycines) feeding on virus-infected soybean SO INSECT MOLECULAR BIOLOGY LA English DT Article DE Aphis glycines; Buchnera aphidicola; tetratrophic interactions; endosymbiont ID LEAF CURL VIRUS; WHITEFLY BEMISIA-TABACI; STREAKED DWARF VIRUS; ACYRTHOSIPHON-PISUM; PEA APHID; SYMBIOTIC BACTERIA; MOSAIC-VIRUS; ENDOSYMBIOTIC BACTERIA; SOGATELLA-FURCIFERA; SCHIZAPHIS-GRAMINUM AB Vertically transmitted bacterial symbionts are common in arthropods. Aphids undergo an obligate symbiosis with Buchnera aphidicola, which provides essential amino acids to its host and contributes directly to nymph growth and reproduction. We previously found that newly adult Aphis glycines feeding on soybean infected with the beetle-transmitted Bean pod mottle virus (BPMV) had significantly reduced fecundity. We hypothesized that the reduced fecundity was attributable to detrimental impacts of the virus on the aphid microbiome, namely Buchnera. To test this, mRNA sequencing and quantitative real-time PCR were used to assay Buchnera transcript abundance and titre in A. glycines feeding on Soybean mosaic virus-infected, BPMV-infected, and healthy soybean for up to 14 days. Our results indicated that Buchnera density was lower and ultimately suppressed in aphids feeding on virus-infected soybean. While the decreased Buchnera titre may be associated with reduced aphid fecundity, additional mechanisms are probably involved. The present report begins to describe how interactions among insects, plants, and plant pathogens influence endosymbiont population dynamics. C1 [Cassone, Bryan J.] Ohio State Univ, OARDC, Ctr Appl Plant Sci, Wooster, OH 44691 USA. [Cassone, Bryan J.; Redinbaugh, Margaret G.; Dorrance, Anne E.] Ohio State Univ, Dept Plant Pathol, OARDC, Wooster, OH 44691 USA. [Redinbaugh, Margaret G.] ARS Corn, USDA, Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. [Michel, Andrew P.] Ohio State Univ, Dept Entomol, OARDC, Wooster, OH 44691 USA. RP Cassone, BJ (reprint author), Ohio State Univ, OARDC, Ctr Appl Plant Sci, Dept Plant Pathol, Wooster, OH 44691 USA. EM cassone.4@osu.edu RI Redinbaugh, Margaret/A-3611-2013 FU Ohio State University, Center for Applied Plant Sciences FX Aphis glycines biotype 3 was kindly provided by Curt Hill, Biotype stock center, UIUC. We thank Brian McSpadden Gardener and Caroline Helene Spence for technical expertise. This research was supported by The Ohio State University, Center for Applied Plant Sciences. NR 75 TC 4 Z9 4 U1 10 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD AUG PY 2015 VL 24 IS 4 BP 422 EP 431 DI 10.1111/imb.12170 PG 10 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CM7OG UT WOS:000357883500003 PM 25845267 ER PT J AU Tuckow, AP Temeyer, KB AF Tuckow, A. P. Temeyer, K. B. TI Discovery, adaptation and transcriptional activity of two tick promoters: Construction of a dual luciferase reporter system for optimization of RNA interference in rhipicephalus (boophilus) microplus cell lines SO INSECT MOLECULAR BIOLOGY LA English DT Article DE biochemistry; molecular genetics; parasitology; gene expression; luciferase; RNAi; Ixodes; Rhipicephalus; ticks; promoter; transcription ID SOUTHERN CATTLE TICK; GENE-EXPRESSION; DROSOPHILA; RESISTANCE; IXODIDAE; ACARI; ACETYLCHOLINESTERASE; BACULOVIRUS; EFFICIENT; GENOMICS AB Dual luciferase reporter systems are valuable tools for functional genomic studies, but have not previously been developed for use in tick cell culture. We evaluated expression of available luciferase constructs in tick cell cultures derived from Rhipicephalus (Boophilus) microplus, an important vector of bovine babesiosis and anaplasmosis. Commercial promoters were evaluated for transcriptional activity driving luciferase expression in the tick cell lines. The human phosphoglycerate kinase (PGK) promoter resulted in detectable firefly luciferase activity within 2 days post-transfection of the R.microplus cell line BME26, with maximal activity at 5 days post-transfection. Several other promoters were weaker or inactive in the tick cells, prompting identification and assessment of transcriptional activity of the homologous ribosomal protein L4 (rpL4, GenBank accession no.: KM516205) and elongation factor 1 (EF-1, GenBank accession no.: KM516204) promoters cloned from R.microplus. Evaluation of luciferase expression driven by various promoters in tick cell culture resulted in selection of the R.microplus rpL4 promoter and the human PGK promoter driving transcription of sequences encoding modified firefly and NanoLuc (R) luciferases for construction of a dual luciferase reporter system for use in tick cell culture. C1 [Tuckow, A. P.; Temeyer, K. B.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Temeyer, KB (reprint author), ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM Kevin.Temeyer@ars.usda.gov NR 46 TC 2 Z9 2 U1 3 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD AUG PY 2015 VL 24 IS 4 BP 454 EP 466 DI 10.1111/imb.12172 PG 13 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CM7OG UT WOS:000357883500006 PM 25892533 ER PT J AU Shelby, KS Perera, OP Snodgrass, GL AF Shelby, K. S. Perera, O. P. Snodgrass, G. L. TI Expression profiles of astakine-like transcripts in the tarnished plant bug, Lygus lineolaris, exposed to fungal spores of Beauveria bassiana SO INSECT MOLECULAR BIOLOGY LA English DT Article DE Lygus lineolaris; real-time PCR; hematopoiesis; circadian; photoperiod ID MISSISSIPPI RIVER DELTA; MIRIDAE POPULATIONS; HETEROPTERA; RESISTANCE; DIET; HEMATOPOIESIS; CRUSTACEAN; CYTOKINES; HESPERUS; ACEPHATE AB Astakines are hematopoietic cytokines originally isolated from crustaceans. We identified three astakine-like transcripts in the tarnished plant bug (Lygus lineolaris), LlAst-1, LlAst-2 and LlAst-3, containing prokineticin domains. Quantitative real-time PCR showed variation in expression patterns of astakines in different tissues and between sexes. Relative expression levels of LlAst-1 were highest in the fat bodies of females, while LlAst-2 expression was highest in the fat bodies of both males and females. LlAst-3 expression was higher in male legs compared with the female legs, but lower in all other tissues. Infection with the entomopathogenic fungus Beauveria bassiana slightly elevated LlAst-1 expression 48 h after infection in both males and females. In contrast, the expression levels of LlAst-2 and LlAst-3 were not significantly changed in males and females. Compared with 12:00 h, LlAst-1 level was higher in both sexes at 18:00 h and 00:00 h (midnight). By 6:00 h, the LlAst-1 level in females was significantly reduced while that in males remained high. LlAst-2 and -3 had highest relative expression levels in females at midnight but were significantly lower than in males at midnight and in both sexes at 18:00 h and 6:00 h. This is the first report of expression of astakine-like cytokines from insects. C1 [Shelby, K. S.] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. [Perera, O. P.; Snodgrass, G. L.] ARS, Southern Insect Management Res Unit, USDA, Stoneville, MS 38776 USA. RP Perera, OP (reprint author), ARS, Southern Insect Management Res Unit, USDA, Stoneville, MS 38776 USA. EM O.P.perera@ars.usda.gov FU Cotton, Inc. [08-471] FX We thank Calvin A Pierce III and Priya Chatakondi (USDA-ARS, SIMRU) for technical support provided in insect rearing, RNA extractions, cDNA synthesis, and cloning Lygus astakine cDNA, Dr Maribel Portilla and Tabatha Nelson (USDA-ARS, SIMRU) for assistance in setting up B. bassiana infection study. All Sanger sequencing was carried out at the core facility of USDA-ARS Genomics and Bioinformatics Research Unit, Stoneville, MS. This work was partially supported by Cotton, Inc. grant 08-471 to OPP. We thank Drs Clint Allen (USDA-ARS SIMRU, Stoneville, MS), Brad Coates (USDA-ARS CICGRU, Ames, IA), and Ryan Kurtz (Cotton Inc., Carey, NC) for critically reading an earlier version this manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 26 TC 0 Z9 0 U1 1 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD AUG PY 2015 VL 24 IS 4 BP 480 EP 490 DI 10.1111/imb.12175 PG 11 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CM7OG UT WOS:000357883500008 PM 26018035 ER PT J AU Ostertag, R Warman, L Cordell, S Vitousek, PM AF Ostertag, Rebecca Warman, Laura Cordell, Susan Vitousek, Peter M. TI Using plant functional traits to restore Hawaiian rainforest SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE community assembly; functional restoration; Hawaiian lowland wet forest; hybrid ecosystem; invasion; multivariate trait space; tropical forest restoration ID ECOSYSTEM C1 [Ostertag, Rebecca] Univ Hawaii, Hilo, HI 96720 USA. [Warman, Laura; Cordell, Susan] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. [Vitousek, Peter M.] Stanford Univ, Stanford, CA 94305 USA. RP Ostertag, R (reprint author), Univ Hawaii, Hilo, HI 96720 USA. EM ostertag@hawaii.edu RI Warman, Laura/C-6651-2011 OI Warman, Laura/0000-0001-7339-749X FU Strategic Environmental Research and Development Program [RC-2117]; National Science Foundation [EPS-0903833] FX We thank the Strategic Environmental Research and Development Program for funding (Project RC-2117). Access to field sites was provided by the County of Hawai'i, Division of Forestry and Wildlife and Hawai'i Army National Guard. Jodie Schulten, Amanda Uowolo, Kai McGuire-Turcotte, Chaunda Tactay and Chris Chu assisted with field data collection and laboratory processing. Analyses were conducted by Lucas Mead and Tara Holitzki at the UH Hilo Analytical Laboratory; they were supported in part by National Science Foundation award number EPS-0903833. We thank our partners in the Hawai'i Army National Guard Environmental Office (Angela Kieran-Vast and Craig Blaisdell) and staff at Keaukaha Military Reservation for facilitating the establishment of the Liko Na Pilina project. Karen Holl provided comments on an earlier draft. NR 18 TC 9 Z9 9 U1 9 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD AUG PY 2015 VL 52 IS 4 BP 805 EP 809 DI 10.1111/1365-2664.12413 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CM9CR UT WOS:000358004300001 ER PT J AU Roncal, J Couderc, M Baby, P Kahn, F Millan, B Meerow, AW Pintaud, JC AF Roncal, Julissa Couderc, Marie Baby, Patrice Kahn, Francis Millan, Betty Meerow, Alan W. Pintaud, Jean-Christophe TI Palm diversification in two geologically contrasting regions of western Amazonia SO JOURNAL OF BIOGEOGRAPHY LA English DT Article DE Arecaceae; Astrocaryum sect; Huicungo; dispersal; diversification time; Fitzcarrald Arch; inter-Andean valleys; lowland rain forests; northern Amazonian foreland basin; phylogeography; vicariance ID LATE MIOCENE; FORELAND BASIN; ASTROCARYUM ARECACEAE; POPULATION-GROWTH; ANDEAN UPLIFT; NAZCA RIDGE; RAIN-FOREST; PHYLOGEOGRAPHY; SPECIATION; NEOTROPICS AB AimThe subduction of the Nazca Plate and the eastward propagation of the Andean orogenic wedge in western Amazonia caused the formation of arches or ridges that have influenced the modern configuration of the upper Amazon drainage and the diversification of biota. We used a lineage of 15 palm species (Astrocaryum sect. Huicungo, Arecaceae) to test two biogeographical hypotheses for lowland plants: (1) that vicariance resulted from tectonically mediated geographical barriers (population contraction), and (2) that recurrent dispersal events (population expansion) produced geographical isolation and subsequent speciation. LocationRain forests of South America. MethodsA total of 78 palm individuals were collected in the field, from which five chloroplast and two nuclear DNA fragments were sequenced. We reconstructed a Bayesian dated phylogeny and inferred the demographic history. We used a Bayesian phylogeographical spatial diffusion approach to propose a model of colonization. ResultsWe found a phylogeographical break at c. 5 degrees S between two main clades with crown ages of c. 6.7 and 7.3Ma located in the Fitzcarrald Arch (FA) and the subsiding northern Amazonian foreland basin (NAFB), respectively. These diversification times were close to the emergence of the FA in the late Miocene, and the coeval development of the transcontinental modern drainage and sedimentation plain of the NAFB. As expected for the recurrent-dispersal hypothesis, lineage delimitations were spatially inconsistent with the location of rivers or ridges, and we found some evidence of past ancestral population expansion supported particularly by the chloroplast sequences. Main conclusionsOur results support the biogeographical scenario whereby recurrent dispersal into western Amazonia produced spatial isolation of populations, followed by speciation within two areas of contrasting geological activity: tectonic uplift in the FA versus subsidence in the NAFB. We did not test and cannot rule out ecological speciation within western Amazonia or at finer spatial scales. C1 [Roncal, Julissa] Mem Univ Newfoundland, Dept Biol, St John, NF A1B 3X9, Canada. [Roncal, Julissa; Couderc, Marie; Pintaud, Jean-Christophe] IRD, UMR DIADE, F-34394 Montpellier 5, France. [Baby, Patrice] Univ Toulouse, CNRS,UMR 5563,UR 234,IRD,UPS Toulouse, GET, CNES, F-31400 Toulouse, France. [Baby, Patrice] Convenio IRD PERUPETRO, Lima 41, Peru. [Kahn, Francis; Millan, Betty] Univ Nacl Mayor San Marcos, Museo Hist Nat, Lima 14, Peru. [Meerow, Alan W.] ARS, USDA, SHRS, Natl Germplasm Repository, Miami, FL 33158 USA. RP Roncal, J (reprint author), Mem Univ Newfoundland, Dept Biol, 232 Elizabeth Ave, St John, NF A1B 3X9, Canada. EM jroncal@mun.ca RI Baby, Patrice/D-2936-2009 OI Baby, Patrice/0000-0001-6142-5174 FU Marie Curie Intra-European Fellowship Program [PIEF-GA-2009-251702]; European Commission [212631]; IRD PPR AMAZ DYNADIV/GET actions FX This study was supported by the Marie Curie Intra-European Fellowship Program (PIEF-GA-2009-251702), the European Commission FP7-PALMS project (Grant Agreement 212631; 2009-2013) and the IRD PPR AMAZ DYNADIV/GET actions in 2012 and 2013. We are grateful to Yolanda Wiersma for earlier versions of the map. Philippe Lemey and Guy Beale helped with the selection of spatial diffusion models. Alexandre Antonelli, Luke Beranek and two anonymous referees provided very helpful comments on the manuscript. NR 73 TC 1 Z9 2 U1 6 U2 50 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0305-0270 EI 1365-2699 J9 J BIOGEOGR JI J. Biogeogr. PD AUG PY 2015 VL 42 IS 8 BP 1503 EP 1513 DI 10.1111/jbi.12518 PG 11 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA CM7TP UT WOS:000357899700013 ER PT J AU Stojkov, J von Keyserlingk, MAG Marchant-Forde, JN Weary, DM AF Stojkov, J. von Keyserlingk, M. A. G. Marchant-Forde, J. N. Weary, D. M. TI Assessment of visceral pain associated with metritis in dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE rectal palpation; back arch; heart rate variability; pain behavior ID HEART-RATE-VARIABILITY; REPRODUCTIVE-PERFORMANCE; UTERINE INFLAMMATION; SPECTRAL-ANALYSIS; HYPERALGESIA; STRESS; WOMEN; CATTLE; VALIDATION; MECHANISMS AB Metritis is a common disease in dairy cattle, but to our knowledge, no work has assessed pain associated. with this disease. Tissue palpation is commonly used to assess pain in human and veterinary medicine. The objective of this study was to evaluate visceral pain responses during rectal palpation, with and without uterine palpation, in healthy cows and in cows diagnosed with metritis. A total of 49 Holstein dairy cows (mean standard deviation parity of 2.8 +/- 1.8) were subjected to systematic health checks every 3 d after parturition for 21 d, scoring for vaginal discharge (0 to 4); 13 cows showed a discharge score >= 2 during at least 1 health check and were classified as metritic, whereas 29 cows were classified as healthy and showed no sign of this or any other disease (including mastitis and lameness). Back arch and heart rate variability before examination and during palpation were recorded using video and heart rate monitors. Back arch (cm(2)) on the day of diagnosis was greater in metritic versus healthy cows (1,034 72 vs. 612 48 cm(2)), and greater during rectal palpation with uterine palpation versus rectal palpation without uterine palpation (869 45 vs. 777 45 cm2). Heart rate frequency domain analysis showed that the low-frequency portion was higher in cows with metritis versus healthy cows (16.5 +/- 1.2 vs. 12.9 +/- 1.0). Time domain analysis showed that the standard deviation between normal to normal interbeat intervals and the root mean square of successive differences both decreased during rectal palpation with uterine palpation versus rectal palpation without uterine palpation (1.9 +/- 0.1 vs. 2.5 +/- 0.1 and 1.3 +/- 0.1 vs. 1.7 +/- 0.1, respectively). Together, these results indicate that the inflammation associated with metritis is painful, and that the pain response can be detected during rectal palpation with and without uterine palpation. Rectal palpation with uterine palpation appears to be more aversive than rectal palpation without uterine palpation, suggesting that the former should be avoided when possible. C1 [Stojkov, J.; von Keyserlingk, M. A. G.; Weary, D. M.] Univ British Columbia, Fac Land & Food Syst, Anim Welf Program, Vancouver, BC V6T 1Z4, Canada. [Marchant-Forde, J. N.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Weary, DM (reprint author), Univ British Columbia, Fac Land & Food Syst, Anim Welf Program, Vancouver, BC V6T 1Z4, Canada. EM danweary@mail.ubc.ca RI Weary, Daniel/A-8868-2010; OI Weary, Daniel/0000-0002-0917-3982; Marchant-Forde, Jeremy/0000-0002-5287-2914 FU NSERC's Industrial Research Chair Program with industry contributions from the Dairy Farmers of Canada (Ottawa, ON, Canada); British Columbia Dairy Association (Burnaby, BC Canada); Westgen Endowment Fund (Milner, BC, Canada); Intervet Canada Corporation (Kirkland, QC, Canada); Zoetis (Kirkland, QC, Canada); BC Cattle Industry Development Fund (Kamloops, BC, Canada); Alberta Milk (Edmonton, AB, Canada); Valacta (St. Anne-de-Bellevue, QC, Canada); Can West DHI (Guelph, ON, Canada); Canada's Natural Sciences and Engineering Research Council (NSERC, Ottawa, ON, Canada) FX We thank the staff and students at The University of British Columbia's Dairy Education and Research Centre. The authors are grateful to Julie Huzzey and Julia Lomb (Animal Welfare Program, Vancouver, BC, Canada) for assisting with handling and data collection and to Doug Viera (Agriculture and Agri-Food Canada, Agassiz, BC, Canada) for lending us the heart rate monitors. This research was funded by a Discovery Grant to D. M. Weary from Canada's Natural Sciences and Engineering Research Council (NSERC, Ottawa, ON, Canada). University of British Columbia's Animal Welfare Program is funded by NSERC's Industrial Research Chair Program with industry contributions from the Dairy Farmers of Canada (Ottawa, ON, Canada), British Columbia Dairy Association (Burnaby, BC Canada), Westgen Endowment Fund (Milner, BC, Canada), Intervet Canada Corporation (Kirkland, QC, Canada), Zoetis (Kirkland, QC, Canada), BC Cattle Industry Development Fund (Kamloops, BC, Canada), Alberta Milk (Edmonton, AB, Canada), Valacta (St. Anne-de-Bellevue, QC, Canada), and Can West DHI (Guelph, ON, Canada). NR 51 TC 4 Z9 4 U1 2 U2 29 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2015 VL 98 IS 8 BP 5352 EP 5361 DI 10.3168/jds.2014-9296 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CM7AF UT WOS:000357842100029 PM 26074240 ER PT J AU Weiss, WP Azem, E Steinberg, W Reinhardt, TA AF Weiss, W. P. Azem, E. Steinberg, W. Reinhardt, T. A. TI Effect of feeding 25-hydroxyvitamin D-3 with a negative cation-anion difference diet on calcium and vitamin D status of periparturient cows and their calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE vitamin D; hypocalcemia; dietary cation-anion difference ID DAIRY-COWS; SUBCLINICAL HYPOCALCEMIA; MILK FEVER; REPRODUCTIVE DISORDERS; PARTURIENT PARESIS; TRANSITION PERIOD; SERUM METABOLITES; RISK-FACTOR; PLASMA; CATTLE AB Holstein cows (>1 gestation) were fed 1 of 3 diets during the last 13 d of gestation (ranged from 22 to 7 d). The control diet (16 cows) was formulated to provide 18,000 IU/d of vitamin D-3 and had a dietary cation-anion difference (DCAD) of 165 mEq/kg (DCAD = Na + K - Cl - S). The second diet (DCAD + D) provided the same amount of vitamin D-3 but had a DCAD of -139 mEq/kg (17 cows). The third diet (DCAD + 25D) had no supplemental vitamin D-3 but provided 6 mg/d of 25(OH) vitamin D-3 [25-(OH)D-3] with a DCAD of -138 mEq/kg (20 cows). Diets were fed until parturition and then all cows were fed a common lactation diet that contained vitamin D-3. Negative DCAD diets reduced urine pH, with the greatest decrease occurring with the DCAD + D treatment. Urinary Ca excretion was greatest for cows fed DCAD + 25D followed by cows fed DCAD + D. Urinary pH was negatively correlated with urinary excretion of Ca for cows fed DCAD + D. No such correlation was observed with the DCAD + 25D treatment because substantial excretion of urinary Ca occurred at moderate urinary pH values for that treatment. Cows fed DCAD + 25D had greater serum concentrations of 25-(OH)D-3 than other treatments from 5 d after supplementation started through 7 d in milk. Concentrations of 1,25-(OH)(2)D-3 in serum were greatest in DCAD + 25D cows starting at 2 d before calving and continued through 7 d in milk. Serum Ca concentrations 5 d before calving were greatest for cows fed DCAD + 25D, but at other time points before and after parturition treatment did not affect serum Ca. Incidence of clinical hypocalcemia was not statistically different between treatments, but cows fed DCAD + 25 had the highest incidence rate (12.5, 0, and 20% for control, DCAD + D, and DCAD + 25D). Calves born from cows fed DCAD + 25D had greater concentrations of 25-(OH)D-3 in serum at birth than calves from other treatments (before colostrum consumption), but concentrations were similar by 3 d of age. Concentrations of 25-(OH)D-3 in colostrum and transition milk were increased by feeding DCAD + 25D, but by 28 d in milk treatment effects no longer existed. Overall, feeding 25-OH vitamin D with a negative DCAD diet increased vitamin D status of the cow and her newborn calf but had minimal effects on calcium status and did not have positive effects on the incidence of hypocalcemia. C1 [Weiss, W. P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA. [Azem, E.; Steinberg, W.] DSM Nutr Prod Ltd, CH-4002 Basel, Switzerland. [Reinhardt, T. A.] USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. RP Weiss, WP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA. EM Weiss.6@osu.edu FU Ohio Agricultural Research and Development Center, The Ohio State University; DSM Nutritional Products Ltd., Basel, Switzerland FX Salaries and research support provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University. Additional research support provided by DSM Nutritional Products Ltd., Basel, Switzerland. NR 46 TC 4 Z9 4 U1 4 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2015 VL 98 IS 8 BP 5588 EP 5600 DI 10.3168/jds.2014-9188 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CM7AF UT WOS:000357842100050 PM 26051311 ER PT J AU Godden, SM Wells, S Donahue, M Stabel, J Oakes, JM Sreevatsan, S Fetrow, J AF Godden, S. M. Wells, S. Donahue, M. Stabel, J. Oakes, J. M. Sreevatsan, S. Fetrow, J. TI Effect of feeding heat-treated colostrum on risk for infection with Mycobacterium avium ssp paratuberculosis, milk production, and longevity in Holstein dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE colostrum; heat treatment; Johne's disease; Mycobacterium avium ssp paratuberculosis; pasteurize ID SUBSP PARATUBERCULOSIS; BOVINE COLOSTRUM; IMMUNOGLOBULIN-G; PASTEURIZATION TEMPERATURES; BATCH PASTEURIZATION; PASSIVE-IMMUNITY; JOHNES-DISEASE; UNITED-STATES; CALVES; LEVEL AB In summer 2007, a randomized controlled field trial was initiated on 6 large Midwest commercial dairy farms to investigate the effect of feeding heat-treated (HT) colostrum on transmission of Mycobacterium avium ssp. paratuberculosis (MAP) and on future milk production and longevity within the herd. On each farm, colostrum was collected daily from fresh cows, pooled, divided into 2 aliquots, and then 1 aliquot was heat-treated in a commercial batch pasteurizer at 60 degrees C for 60 mm. A sample from each batch of colostrum was collected for PCR testing (MAP-positive vs. MAP-negative). Newborn heifer calves were removed from the dam within 30 to 60 min of birth and systematically assigned to be fed 3.8 L of either fresh (FR; n = 434) or heat-treated (HT; n = 490) colostrum within 2 h of birth. After reaching adulthood (>2 yr old), study animals were tested once annually for 3 yr (2010, 2011, 2012) for infection with MAP using serum ELISA and fecal culture. Lactation records describing milk production data and death or culling events were collected during the 3-yr testing period. Multivariable model logistic and linear regression was used to investigate the effect of feeding HT colostrum on risk for testing positive to MAP during the 3-yr testing period (positive/negative; logistic regression) and on first and second lactation milk yield (kg/cow; linear regression), respectively. Cox proportional hazards regression was used to investigate the effect of feeding HT colostrum on risk and time to removal from the herd. Fifteen percent of all study animals were fed PCR-positive colostrum. By the end of the 3-yr testing period, no difference was noted in the proportion of animals testing positive for MAP, with either serum ELISA or fecal culture, when comparing the HT group (10.5%) versus the FR group (8.1%). There was no effect of treatment on first- (HT = 11.797 kg; FR = 11,671 kg) or second-lactation (HT = 11,013 kg; FR = 11,235 kg) milk production. The proportion of cows leaving the herd by study conclusion was not different for animals originally fed HT (68.0%) versus FR (71.7%) colostrum. Although a previous study showed that feeding HT colostrum (60 degrees C for 60 min) produces short-term benefits, including improved passive transfer of IgG and reduced morbidity in the preweaning period, the current study found no benefit of feeding HT colostrum on long-term outcomes including risk for transmission of Mycobacterium avium ssp. paratuberculosis, milk production in the first and second lactation, and longevity within the herd. C1 [Godden, S. M.; Wells, S.; Donahue, M.; Sreevatsan, S.; Fetrow, J.] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. [Stabel, J.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Oakes, J. M.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55454 USA. RP Godden, SM (reprint author), Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. EM godde002@umn.edu OI Sreevatsan, Srinand/0000-0002-5162-2403 FU USDA-Cooperative State Research, Education, and Extension Service (Washington, DC) FX Phase I of this study (calf enrollment to weaning) was funded by USDA-Cooperative State Research, Education, and Extension Service (Washington, DC). This project was administered by Penn State University (University Park, PA) and the College of Veterinary Medicine, University of Minnesota. The authors thank the owners and managers of the 6 dairy herds for their participation and support. We also thank the many students who provided technical assistance throughout the study, including Eb Ballinger, Amber Hazel, Katie Konkol, Jennifer Belz, Amy Dahlke, and Maggie Wehseler (College of Veterinary Medicine, University of Minnesota. St. Paul). NR 42 TC 2 Z9 2 U1 5 U2 22 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2015 VL 98 IS 8 BP 5630 EP 5641 DI 10.3168/jds.2015-9443 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CM7AF UT WOS:000357842100053 PM 26074228 ER PT J AU Broderick, GA Faciola, AP Armentano, LE AF Broderick, Glen A. Faciola, Antonio P. Armentano, Louis E. TI Replacing dietary soybean meal with canola meal improves production and efficiency of lactating dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE canola meal; soybean meal; milk production; nitrogen efficiency ID RUMEN-PROTECTED METHIONINE; DIFFERENT PROTEIN SUPPLEMENTS; MILK UREA NITROGEN; HIGH-MOISTURE CORN; ALFALFA SILAGE; AMINO-ACIDS; RUMINAL DEGRADABILITY; NUTRIENT UTILIZATION; DETERGENT FIBER; SOLE FORAGE AB Previous research suggested that crude protein (CP) from canola meal (CM) was used more efficiently than CP from solvent soybean meal (SBM) by lactating dairy cows. We tested whether dietary CP content influenced relative effectiveness of equal supplemental CP from either CM or SBM. Fifty lactating Holstein cows were blocked by parity and days in milk into 10 squares (2 squares with ruminal cannulas) in a replicated 5 x 5 Latin square trial. Five squares were fed: (1) low (14.5-14.8%) CP with SBM, (2) low CP with CM, (3) low CP with SBM plus CM, (4) high (16.4-16.7%) CP with SBM, and (5) high CP with CM; the other 5 squares were fed the same diets except with rumen-protected Met plus Lys (RPML) added as Mepron (Degussa Corp., Kennesaw, GA) and AminoShure-L (Balchem Corp., New Hampton, NY), which were assumed to provide 8 g/d of absorbed DL-Met and 12 g/d of absorbed L-Lys. Diets contained [dry matter (DM) basis] 40% corn silage, 26% alfalfa silage, 14 to 23% corn grain, 2.4% mineral-vitamin premixes, and 29 to 33% neutral detergent fiber. Periods were 3 wk (total 15 wk), and data from the last week of each period were analyzed using the Mixed procedures of SAS (SAS Institute Inc., Cary, NC). The only effects of RPML were increased DM intake and milk urea N (MUN) and urinary N excretion and trends for decreased milk lactose and solids-not-fat concentrations and milk-N:N intake; no significant RPML x protein source interactions were detected. Higher dietary CP increased milk fat yield and tended to increase milk yield but also elevated MUN, urine volume, urinary N excretion, ruminal concentrations of ammonia and branched-chain volatile fatty acids (VFA), lowered milk lactose concentration and milk-N:N intake, and had no effect on milk true protein yield. Feeding CM instead of SBM increased feed intake, yields of milk, energy-corrected milk, and true protein, and milk-N:N intake, tended to increase fat and lactose yields, and reduced MUN, urine volume, and urinary N excretion. At low CP, MUN was lower and intake tended to be greater on SBM plus CM versus SBM alone, but MUN and N excretion were not reduced to the same degree as on CM alone. Interactions of parity x protein source and parity x CP concentration indicated that primiparous cows were more responsive than multiparous cows to improved supply of metabolizable protein. Replacing SBM with CM reduced ruminal ammonia and branched-chain VFA concentrations, indicating lower ruminal degradation of CM protein. Replacing SBM with CM improved milk and protein yield and N-utilization in lactating cows fed both low- and high-CP diets. C1 [Broderick, Glen A.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Faciola, Antonio P.] Univ Nevada, Dept Agr Nutr & Vet Sci, Reno, NV 89557 USA. [Armentano, Louis E.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Broderick, Glen A.] Broderick Nutr & Res LLC, Madison, WI 53705 USA. RP Broderick, GA (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM gbroderi@wisc.edu FU Canola Council of Canada (Winnipeg, MB, Canada) FX The authors thank Rick Walgenbach and his farm crew (US Dairy Forage Research Center, Madison, WI) for harvesting and storing the feeds used in this trial and Nancy Betzold and her barn crew at the US Dairy Forage Research Center Farm (Prairie du Sac, WI) for feeding and animal care; Wendy Radloff and Mary Becker of the US Dairy Forage Research Center (Madison, WI) for assisting in sampling and laboratory analyses; Peter Crump of the University of Wisconsin-Madison for assisting with statistical analyses; and the Canola Council of Canada (Winnipeg, MB, Canada) for partial funding of this project. NR 57 TC 5 Z9 5 U1 6 U2 25 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2015 VL 98 IS 8 BP 5672 EP 5687 DI 10.3168/jds.2015-9563 PG 16 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CM7AF UT WOS:000357842100056 PM 26074230 ER PT J AU Anderson, JL Kalscheur, KF Garcia, AD Schingoethe, DJ AF Anderson, J. L. Kalscheur, K. F. Garcia, A. D. Schingoethe, D. J. TI Feeding fat from distillers dried grains with solubles to dairy heifers: I. Effects on growth performance and total-tract digestibility of nutrients SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE distillers grains; dairy heifer; dietary fat ID HOLSTEIN HEIFERS; PLUS SOLUBLES; PRODUCTS; FORAGE; DIETS; SIZE AB The objective of this study was to determine if increased dietary fat from dried distillers grains with solubles (DDGS) in diets of growing heifers affected dry matter intake, average daily gain (ADG), growth performance, and nutrient digestibility. Thirty-three Holstein heifers (133 +/- 18 d old) were used in a 24-wk randomized complete block design. Treatments we re (1) control (CON) containing ground corn and soybean products, (2) low-fat (LFDG) containing low-fat, high-protein DDGS and ground corn, and (3) high-fat (HFDG) with traditional DDGS. All diets contained 39.8% grass hay, 24.8% corn silage, and 1.5% vitamins and minerals. The HFDG diet was formulated to contain 4.8% fat compared with 2.8% in the CON and LFDG diets, which were greater in nonfibrous carbohydrate. Diets had a net energy gain of 1.0 Mcal/kg of dry matter and were limit-fed at 2.45% of body weight. Heifers were weighed every 2 wk and rations were adjusted accordingly. Heart girth, hip and wither heights, body length, and body condition score were recorded every 2 wk. Total-tract digestion of nutrients was evaluated during wk 16 using fecal grab sampling and an external marker. No treatments by time interactions were found. Dry matter intakes, body weights, ADG, and gain-to-feed ratio were similar among treatments; however, ADG averaged 0.96 kg/d among treatments, which is greater than recommended. All body frame measurements and body condition scores were similar among treatments. Total-tract digestibilities of dry matter and organic matter were not different among treatments. However, crude protein and neutral detergent fiber digestibility were increased in the HFDG diet compared with the CON and LFDG diets. These results demonstrate that using DDGS or low-fat DDGS with corn in growing heifer rations can maintain performance. Utilizing the fat in DDGS as a dietary energy source in replacement of starch from corn did not influence growth performance or negatively affect nutrient digestion.y C1 [Anderson, J. L.; Kalscheur, K. F.; Garcia, A. D.; Schingoethe, D. J.] S Dakota State Univ, Dept Dairy Sci, Brookings, SD 57007 USA. RP Kalscheur, KF (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. EM kenneth.kalscheur@ars.usda.gov FU South Dakota Agriculture Experiment Station (Brookings); USDA-Agricultural Research Service (Washington, DC) [58-5447-7-322, NC-1042] FX This work was supported in part by the South Dakota Agriculture Experiment Station (Brookings) and USDA-Agricultural Research Service (Washington, DC) Agreement No. 58-5447-7-322, and was a component of NC-1042: Management Systems to Improve the Economic and Environmental Sustainability of Dairy Enterprises. In memoriam, the authors gratefully acknowledge the role that Arnold Hippen had in helping to plan this research. The authors also thank H. Bruns, F. Diaz-Royon, K. Herrick, K. Mjoun, S. Ranathunga (who were graduate students in the South Dakota State University dairy science department at the time of the study), and the South Dakota State University Dairy Research and Training Facility farm crew for assistance with the farm trial. NR 25 TC 4 Z9 4 U1 2 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2015 VL 98 IS 8 BP 5699 EP 5708 DI 10.3168/jds.2014-9162 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CM7AF UT WOS:000357842100058 PM 26074227 ER PT J AU Ma, JT Fox, CS Jacques, PF Speliotes, EK Hoffmann, U Smith, CE Saltzman, E McKeown, NM AF Ma, Jiantao Fox, Caroline S. Jacques, Paul F. Speliotes, Elizabeth K. Hoffmann, Udo Smith, Caren E. Saltzman, Edward McKeown, Nicola M. TI Sugar-sweetened beverage, diet soda, and fatty liver disease in the Framingham Heart Study cohorts SO JOURNAL OF HEPATOLOGY LA English DT Article DE Sugar-sweetened beverages; Diet soda; Alanine transaminase; Fatty liver disease ID FOOD FREQUENCY QUESTIONNAIRE; VISCERAL FAT; CARDIOVASCULAR-DISEASE; METABOLIC SYNDROME; AMINOTRANSFERASE LEVELS; COMPUTED-TOMOGRAPHY; INSULIN-RESISTANCE; UNITED-STATES; PPAR-ALPHA; FRUCTOSE AB Background & Aims: Non-alcoholic fatty liver disease affects similar to 30% of US adults, yet the role of sugar-sweetened beverages and diet soda on these diseases remains unknown. We examined the cross-sectional association between intake of sugar-sweetened beverages or diet soda and fatty liver disease in participants of the Framingham Offspring and Third Generation cohorts. Methods: Fatty liver disease was defined using liver attenuation measurements generated from computed tomography in 2634 participants. Alanine transaminase concentration, a crude marker of fatty liver disease, was measured in 5908 participants. Sugar-sweetened beverage and diet soda intake were estimated using a food frequency questionnaire. Participants were categorized as either non-consumers or consumers (3 categories: 1 serving/month to <1 serving/week, 1 serving/week to <1 serving/day, and P1 serving/day) of sugar-sweetened beverages or diet soda. Results: After adjustment for age, sex, smoking status, Framingham cohort, energy intake, alcohol, dietary fiber, fat (% energy), protein (% energy), diet soda intake, and body mass index, the odds ratios of fatty liver disease were 1, 1.16 (0.88, 1.54), 1.32 (0.93, 1.86), and 1.61 (1.04, 2.49) across sugar-sweetened beverage consumption categories (p trend = 0.04). Sugar-sweetened beverage consumption was also positively associated with alanine transaminase levels (p trend = 0.007). We observed no significant association between diet soda intake and measures of fatty liver disease. Conclusion: In conclusion, we observed that regular sugar-sweetened beverage consumption was associated with greater risk of fatty liver disease, particularly in overweight and obese individuals, whereas diet soda intake was not associated with measures of fatty liver disease. (C) 2015 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. C1 [Ma, Jiantao; Jacques, Paul F.; Smith, Caren E.; Saltzman, Edward; McKeown, Nicola M.] Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fox, Caroline S.] NHLBIs Framingham Heart Study, Framingham, MA USA. [Fox, Caroline S.; Hoffmann, Udo] Harvard Univ, Sch Med, Boston, MA USA. [Speliotes, Elizabeth K.] Univ Michigan, Sch Med, Ann Arbor, MI USA. [Hoffmann, Udo] Massachusetts Gen Hosp, Boston, MA 02114 USA. RP McKeown, NM (reprint author), Tufts Univ, Jean Mayer USDA HNRCA, 711 Washington St, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU NIH/NHLBI's Framingham Heart Study [N01-HC-25195]; Boston University School of Medicine; United States Department of Agriculture, Agricultural Research Service (USDA) [58-1950-0-014]; NIH [HL112845] FX This project has been funded by the NIH/NHLBI's Framingham Heart Study (Contract N01-HC-25195); the Boston University School of Medicine; and the United States Department of Agriculture, Agricultural Research Service (USDA Agreement No. 58-1950-0-014). C.E.S was supported by the NIH K08 award (HL112845). NR 47 TC 16 Z9 16 U1 1 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-8278 EI 1600-0641 J9 J HEPATOL JI J. Hepatol. PD AUG PY 2015 VL 63 IS 2 BP 462 EP 469 DI 10.1016/j.jhep.2015.03.032 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA CM8YX UT WOS:000357991700024 PM 26055949 ER PT J AU Morel, JL Charzynski, P Shaw, RK Zhang, GL AF Morel, Jean Louis Charzynski, Przemysaw Shaw, Richard K. Zhang, Ganlin TI The seventh SUITMA conference held in ToruA", Poland, September 2013 PREFACE SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Editorial Material DE IUSS working group; SUITMA; Urban soils C1 [Morel, Jean Louis] Univ Lorraine, Lab Sols & Environm, F-54518 Vandoeuvre Les Nancy, France. [Charzynski, Przemysaw] Nicolaus Copernicus Univ, Fac Earth Sci, Dept Soil Sci & Landscape Management, PL-87100 Torun, Poland. [Shaw, Richard K.] USDA Nat Resources Conservat Serv, NRCS New Jersey, Somerset, NJ 08873 USA. [Zhang, Ganlin] Chinese Acad Sci, Inst Soil Sci, Dept Soil Resources & Remote Sensing Applicat, Nanjing 210008, Jiangsu, Peoples R China. RP Morel, JL (reprint author), Univ Lorraine, Lab Sols & Environm, 2 Ave Foret Haye, F-54518 Vandoeuvre Les Nancy, France. EM Jean-Louis.Morel@univ-lorraine.fr RI Charzynski, Przemyslaw/E-7680-2014; OI Charzynski, Przemyslaw/0000-0003-1467-9870; Morel, Jean Louis/0000-0002-5720-1482 NR 4 TC 1 Z9 1 U1 0 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD AUG PY 2015 VL 15 IS 8 BP 1657 EP 1658 DI 10.1007/s11368-015-1184-5 PG 2 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA CM9XO UT WOS:000358063700001 ER PT J AU Villasante, A Patro, B Chew, B Becerra, M Wacyk, J Overturf, K Powell, MS Hardy, RW AF Villasante, Alejandro Patro, Biswamitra Chew, Boon Becerra, Michael Wacyk, Jurij Overturf, Ken Powell, Madison S. Hardy, Ronald W. TI Dietary Intake of Purple Corn Extract Reduces Fat Body Content and Improves Antioxidant Capacity and n-3 Polyunsaturated Fatty Acid Profile in Plasma of Rainbow Trout, Oncorhynchus mykiss SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID LIPID OXIDATION-PRODUCTS; OXIDIZED FISH-OIL; SALMO-SALAR L.; GROWTH-PERFORMANCE; VITAMIN-E; IN-VITRO; DICENTRARCHUS-LABRAX; NUTRITIVE-VALUE; TISSUE-DAMAGE; LDL OXIDATION AB A study was conducted to determine the benefits of the intake of purple corn extract (PCE), a natural source of anthocyanins, in rainbow trout, Oncorhynchus mykiss. A control or test diet supplemented with 5% PCE was fed to triplicate tanks with 25 fish each for 8 wk. The concentrations of three anthocyanins, cyanidin-3-glucoside, pelargonidin-3-glucoside, and peonidin-3-glucoside, were measured in PCE. The chemical composition and fatty acid profiles of fish, as well as the fatty acid profiles in plasma, liver, and muscle were determined. The total antioxidant capacity (TAC) and the concentration of oxidative damage biomarkers, for example, protein carbonyls, 8-hydroxydeoxyguanosine, and thiobarbituric acid reactive substances (TBARS), were determined in plasma. Transcription of two antioxidant enzymes, glutathione peroxidase 1 (gpx1) and superoxide dismutase 1 (sod1), was measured in erythrocytes. Significant lower adiposity and significant higher percentage of total n-3 and total n-6 polyunsaturated fatty acids (PUFA) in the body of fish fed the test diet were detected. Significant higher plasma percentage of total n-3 PUFA, significant higher plasma TAC, and significant higher expression of gpx1 in erythrocytes of fish fed the test diet were measured. Overall, our results suggest potential protection against in vivo lipid peroxidation in fish fed the PCE supplemented diet. This is especially true due to detection of an enhanced antioxidant protection in plasma and erythrocytes in fish, the reduced adiposity and greater proportion of total n-3 and n-6 PUFA in the fish body, as well as a tendency toward lower TBARS plasma concentration in fish fed the test diet when compared to the control group. C1 [Villasante, Alejandro; Patro, Biswamitra; Powell, Madison S.; Hardy, Ronald W.] Univ Idaho, Inst Aquaculture Res, Hagerman, ID 83332 USA. [Chew, Boon] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Becerra, Michael; Overturf, Ken] USDA ARS, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Wacyk, Jurij] Univ Chile, Fac Ciencias Agron, Dept Anim Prod, Santiago 1004, Chile. RP Villasante, A (reprint author), Univ Idaho, Inst Aquaculture Res, Hagerman, ID 83332 USA. EM vill0378@vandals.uidaho.edu RI Wacyk, Jurij/B-6544-2014 OI Wacyk, Jurij/0000-0002-9044-2755 NR 85 TC 3 Z9 5 U1 2 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD AUG PY 2015 VL 46 IS 4 BP 381 EP 394 DI 10.1111/jwas.12206 PG 14 WC Fisheries SC Fisheries GA CM8TQ UT WOS:000357975400003 ER PT J AU Sealey, WM O'Neill, TJ Peach, JT Gaylord, TG Barrows, FT Block, SS AF Sealey, Wendy M. O'Neill, Thomas J. Peach, Jesse T. Gaylord, T. Gibson Barrows, Frederic T. Block, Stephanie S. TI Refining Inclusion Levels of Grain Distiller's Dried Yeast in Commercial-type and Plant-based Diets for Juvenile Rainbow Trout, Oncorhynchus mykiss SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID COBIA RACHYCENTRON-CANADUM; FISH-MEAL; CANDIDA-UTILIS; PROTEIN-SOURCE; ETHANOL YEAST; SUNSHINE BASS; GROWTH; REPLACEMENT; PERFORMANCE; FEEDS AB Recent studies with rainbow trout, Oncorhynchus mykiss, demonstrated that grain distiller's dried yeast (GDDY) at up to 12% inclusion was an effective dietary protein source when replacing fish meal. To examine the effectiveness of GDDY when replacing an increased variety of dietary protein sources, two feeding trials were conducted. In the first trial, six commercial-type diets were formulated to contain 42% digestible protein and 20% crude lipid with GDDY included at 0, 6, 9, 12, 15, and 18% to replace all dietary protein sources except fish meal. In the second trial, four plant-based diets with GDDY at 0, 9, 12, and 15% were examined. Experimental diets were fed twice daily, to apparent satiation, to three replicate tanks of fish/diet (initial weight = 22.3 +/- 0.7 g) for 10 wk in a 15 C recirculating system. Results demonstrated excellent fish growth and the inclusion rate of GDDY did not negatively affect growth or feed conversion. No significant negative effects of GDDY on body indices or whole-body proximate composition were observed. Based on these results, GDDY can be included in both commercial-type diets and plant-based diets for rainbow trout at up to 18 or 15%, respectively, without decreasing growth performance. C1 [Sealey, Wendy M.; O'Neill, Thomas J.; Peach, Jesse T.; Gaylord, T. Gibson] USFWS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Barrows, Frederic T.] USDA ARS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Block, Stephanie S.] Archer Daniel Midlands Res, Decatur, IL 62521 USA. RP Sealey, WM (reprint author), USFWS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. EM Wendy_Sealey@fws.gov NR 40 TC 1 Z9 1 U1 3 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD AUG PY 2015 VL 46 IS 4 BP 434 EP 444 DI 10.1111/jwas.12199 PG 11 WC Fisheries SC Fisheries GA CM8TQ UT WOS:000357975400007 ER PT J AU Lustgarten, MS Price, LL Fielding, RA AF Lustgarten, Michael S. Price, Lori Lyn Fielding, Roger A. TI Analytes and Metabolites Associated with Muscle Quality in Young, Healthy Adults SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article ID SKELETAL-MUSCLE; ADAPTIVE RESPONSE; OLDER-ADULTS; AMINO-ACID; EXERCISE; WOMEN; IDENTIFICATION; ACTIVATION; METABOLOMICS; PERFORMANCE AB Purpose Identification of mechanisms that underlie lower extremity muscle quality (leg press one repetition maximum/total lean mass; LP/Lean) may be important for individuals interested in optimizing fitness and sport performance. The purpose of the current study was to provide observational insight into mechanisms that may underlie muscle quality by characterizing the association between 286 mass spectrometry metabolites and 17 chemistry screen analytes with LP/Lean in young, healthy adults (N = 77 (49 women and 28 men); mean age, 24.4 +/- 4.2 yr; BMI, 23.5 +/- 2.6 kg center dot m(-2)). Methods Principal components analysis (PCA) was used to reduce the 286 metabolites into 73 metabolite-containing PCA factors. Sex-adjusted linear regression was used to examine the association between PCA factors and chemistry screen analytes with LP/Lean. Q values were computed to account for multiple comparison testing. Stepwise linear regression and leave-one-out cross validation were used to identify a predictor set representative of LP/Lean and to assess internal validity, respectively. Results Metabolites or analytes related to dietary protein intake (albumin, branched-chain amino acids (BCAA)) and excitation-contraction coupling (calcium and magnesium) were positively associated, whereas metabolites related to gut bacterial metabolism (cinnamoylglycine, hydrocinnamate, hippurate, indolepropionate) and peroxisome proliferator-activated receptor-alpha (PPAR-alpha) (methylglutarylcarnitine and cinnamoylglycine) activation were negatively associated with LP/Lean. Use of leave-one-out cross validation identified magnesium, sex, and the PCA factors containing BCAAs and methionine and methylglutarylcarnitine to be present in more than 90% of the stepwise regression models, thereby explaining 26.7% of the variance (adjusted R-2) inherent in muscle quality. Conclusion Collectively, these data suggest that mechanisms related to dietary protein intake, excitation-contraction coupling, gut microbial metabolism, and PPAR-alpha activation may underlie lower extremity muscle quality in young, healthy adults. C1 [Lustgarten, Michael S.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Price, Lori Lyn] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Biostat Res Ctr, Boston, MA USA. RP Fielding, RA (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Roger.Fielding@tufts.edu FU DOD [W911SR06C0001]; US Department of Agriculture [58-1950-0-014] FX This work was supported by DOD contract #W911SR06C0001 and in part by the US Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture and do not constitute endorsement by American College of Sports Medicine. NR 39 TC 1 Z9 1 U1 1 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0195-9131 EI 1530-0315 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD AUG PY 2015 VL 47 IS 8 BP 1659 EP 1664 DI 10.1249/MSS.0000000000000578 PG 6 WC Sport Sciences SC Sport Sciences GA CM8IF UT WOS:000357942200013 PM 25412292 ER PT J AU Chandra, A Keizerweerd, AT Que, YX Grisham, MP AF Chandra, Amaresh Keizerweerd, Amber T. Que, Youxiong Grisham, Michael P. TI Loop-mediated isothermal amplification (LAMP) based detection of Colletotrichum falcatum causing red rot in sugarcane SO MOLECULAR BIOLOGY REPORTS LA English DT Article DE Colletotrichum falcatum; LAMP; Red rot; Sugarcane ID POLYMERASE-CHAIN-REACTION; XYLI SUBSP XYLI; MOLECULAR-DETECTION; RAPID DETECTION; RT-LAMP; VIRUS; DISEASE; ASSAY; DNA AB Red rot, caused by Colletotrichum falcatum, is a destructive disease prevalent in most sugarcane-producing countries. Disease-free sugarcane planting materials (setts) are essential as the pathogen spreads primarily through infected setts. The present study was undertaken to develop a loop-mediated isothermal amplification (LAMP) assay for the detection of C. falcatum. C. falcatum genomic DNA was isolated from pure mycelium culture and infected tissues. Four sets of primers corresponding to a unique DNA sequence specific to C. falcatum were designed. Specificity of the LAMP test was checked with DNA of another fungal pathogen of sugarcane, Puccinia melanocephala, as well as two closely-related species, Colletotrichum fructivorum and Colletotrichum acutatum. No reaction was found with the three pathogens. When C. falcatum DNA from pure culture was used in a detection limit analysis, sensitivity of the LAMP method was observed to be ten times higher than that of conventional PCR; however, sensitivity was only 5 times higher when DNA from C. falcatum-infected tissues was used. Using the LAMP assay, C. falcatum DNA is amplified with high specificity, efficiency, and rapidity under isothermal conditions. Moreover, visual judgment of color change in < 1 h without further post-amplification processing makes the LAMP method convenient, economical, and useful in diagnostic laboratories and the field. C1 [Chandra, Amaresh; Keizerweerd, Amber T.; Grisham, Michael P.] ARS, USDA, MSA, Sugarcane Res Lab, Houma, LA 70360 USA. [Chandra, Amaresh] Indian Inst Sugarcane Res, Lucknow 226002, Uttar Pradesh, India. [Que, Youxiong] Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou 350002, Fujian, Peoples R China. RP Keizerweerd, AT (reprint author), ARS, USDA, MSA, Sugarcane Res Lab, Houma, LA 70360 USA. EM amber.keizerweerd@ars.usda.gov FU Department of Biotechnology, Government of India, New Delhi, India [05/AC/2012-12]; United States Department of Agriculture, Agriculture Research Service (CRIS Project) [6410-22000-013-00D] FX Amaresh Chandra is grateful to the Department of Biotechnology, Government of India, New Delhi, India for awarding the DBT-CREST Fellowship (BT/IN/DBT-CREST Awards/05/AC/2012-12). Amber Keizerweerd and Michael Grisham are thankful for financial support provided by the United States Department of Agriculture, Agriculture Research Service (CRIS Project: 6410-22000-013-00D). Drs. J. W. Hoy and Vinson Doyle are greatly appreciated for their help in providing the C. falcatum, C. fructivorum, and C. acutatum isolates. We also thank Dr. Duncan Clark of OptiGene Limited for his assistance in primer design. NR 32 TC 1 Z9 1 U1 3 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0301-4851 EI 1573-4978 J9 MOL BIOL REP JI Mol. Biol. Rep. PD AUG PY 2015 VL 42 IS 8 BP 1309 EP 1316 DI 10.1007/s11033-015-3875-9 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CN1GD UT WOS:000358165300008 PM 25861736 ER PT J AU Haag, WR Stoeckel, JA AF Haag, Wendell R. Stoeckel, James A. TI The role of host abundance in regulating populations of freshwater mussels with parasitic larvae SO OECOLOGIA LA English DT Article DE Recruitment; Acquired immunity; Competition; Frequency-dependent transmission; Fecundity ID DEPENDENT TRANSMISSION; BIVALVIA-UNIONIDAE; DYNAMICS; FISH; COMMUNITIES; STRATEGIES; GLOCHIDIA; EVOLUTION; DENSITIES; FECUNDITY AB Host-parasite theory makes predictions about the influence of host abundance, competition for hosts, and parasite transmission on parasite population size, but many of these predictions are not well tested empirically. We experimentally examined these factors in ponds using two species of freshwater mussels with parasitic larvae that infect host fishes via different infection strategies. For both species, recruitment and larval survival were positively related to host abundance, but there was no apparent minimum host threshold and positive population growth occurred when an average of one fish per mussel was present. Recruitment increased rapidly with an initial increase in host abundance but appeared to approach an asymptote at moderate host abundance. Recruitment and larval survival did not differ according to whether mussel species occurred alone or in combination, providing no evidence for competition for hosts via acquired immunity. However, larval survival of the species that attracts hosts with a lure was higher than the species that infects hosts passively, but lower survival of the latter strategy was offset by higher fecundity, which resulted in comparable recruitment between the two species. The lack of evidence for competition for hosts or host saturation suggests that mussel recruitment is limited primarily by fecundity and larval transmission efficiency. Despite the lack of a minimum host abundance threshold, high variation in recruitment in all treatments suggests that population growth at low host abundance is limited by stochasticity. These results show that host-parasite interactions in natural situations may differ substantially from predictions based on models or laboratory findings. C1 [Haag, Wendell R.] US Forest Serv, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. [Stoeckel, James A.] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. RP Haag, WR (reprint author), US Forest Serv, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. EM whaag@fs.fed.us FU US Forest Service, Southern Research Station FX We thank S. Nichols and M. Hart, who assisted with all aspects of the experiments. We also thank the following people for their various contributions to the study: M. Bland, A. Commens-Carson, D. Foree, A. Gascho Landis, R. Kennedy, T. Mosley, G. Moyer, I. Palmer, N. Rankin, B. Staton, and M. Warren. This study was supported by the US Forest Service, Southern Research Station. NR 39 TC 2 Z9 2 U1 10 U2 38 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD AUG PY 2015 VL 178 IS 4 BP 1159 EP 1168 DI 10.1007/s00442-015-3310-x PG 10 WC Ecology SC Environmental Sciences & Ecology GA CN0FL UT WOS:000358089400016 PM 25868661 ER PT J AU George, AD Thompson, FR Faaborg, J AF George, Andrew D. Thompson, Frank R., III Faaborg, John TI Isolating weather effects from seasonal activity patterns of a temperate North American Colubrid SO OECOLOGIA LA English DT Article DE Climate change; Elaphe obsoleta; Pantherophis obsoletus; Thermal ecology; Western ratsnakes ID BLACK RAT SNAKES; NEST PREDATION; CLIMATE-CHANGE; ELAPHE-OBSOLETA; SPATIAL ECOLOGY; HABITAT SELECTION; SONGBIRD NESTS; HOME RANGES; RATSNAKES; BEHAVIOR AB Forecasting the effects of climate change on threatened ecosystems and species will require an understanding of how weather influences processes that drive population dynamics. We have evaluated weather effects on activity patterns of western ratsnakes, a widespread predator of birds and small mammals in eastern North America. From 2010-2013 we radio-tracked 53 ratsnakes in the fragmented region of central Missouri. We relocated each snake 4x per week and used movement frequency as an index of activity. We used generalized linear mixed models within an information-theoretic approach to evaluate temporal and weather variables as potential predictors of snake activity. While snakes were generally sedentary, activity showed a linear response to relative humidity and a quadratic response to air temperature, peaking near 30 A degrees C. Seasonal activity patterns differed between sexes and among years, but snakes were generally least active in mid-summer, regardless of weather. Our findings provide strong evidence that air temperature and relative humidity differentially affect activity patterns of an important predator and are the mechanism explaining increased nest predation rates with warmer temperatures. C1 [George, Andrew D.; Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] Univ Missouri, US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. RP George, AD (reprint author), Univ Missouri, Div Biol Sci, 105 Tucker Hall, Columbia, MO 65211 USA. EM adgggb@mail.missouri.edu FU U.S.D.A. Forest Service Northern Research Station; University of Missouri FX We thank A. Mueller, A. Zack, A. Cave, S. Mayne, and J. Wheeler who assisted with the field work. J. Ballard and S. Szepanski helped with transmitter implantation. R. Semlitsch and three anonymous reviewers provided constructive comments to an earlier draft of this manuscript. Funding for this project was provided by the U.S.D.A. Forest Service Northern Research Station and the University of Missouri. NR 61 TC 2 Z9 2 U1 2 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD AUG PY 2015 VL 178 IS 4 BP 1251 EP 1259 DI 10.1007/s00442-015-3300-z PG 9 WC Ecology SC Environmental Sciences & Ecology GA CN0FL UT WOS:000358089400024 PM 25842295 ER PT J AU Hsia, DS Tarai, SG Alimi, A Coss-Bu, JA Haymond, MW AF Hsia, Daniel S. Tarai, Sarah G. Alimi, Amir Coss-Bu, Jorge A. Haymond, Morey W. TI Fluid management in pediatric patients with DKA and rates of suspected clinical cerebral edema SO PEDIATRIC DIABETES LA English DT Article DE cerebral edema; diabetic ketoacidosis; practice guidelines ID CHILDHOOD DIABETIC-KETOACIDOSIS; BRAIN HERNIATION; CONSENSUS STATEMENT; RISK-FACTORS; CHILDREN; THERAPY; ADOLESCENTS; SOCIETY AB ObjectiveTo compare outcomes of diabetic ketoacidosis (DKA) 6 yrs before and 6 yrs after changing rehydration fluids from 1/2 normal saline to Lactated Ringer's and decreasing the total intended fluid volume administered in the first 24 hrs from 3500 mL/m(2)/d to 2500 mL/m(2)/d at Texas Children's Hospital (TCH) in response to recommendations by the ESPE, LWPES, and ISPAD in 2004. Subjects/MethodsA retrospective cohort study was conducted in which 1868 admissions for DKA were identified and reviewed. The cohort was divided into two groups: Group A, 1998-2004, and Group B, 2004-2010. Subjects with suspected clinical cerebral edema and adverse outcomes were identified. ResultsAlthough not statistically significant, there was an equal number (n = 3) of adverse outcomes (death or neurological damage) in each group despite more than double the admissions in Group B (1264) compared with those in Group A (604). Overall, the incidence of suspected clinical cerebral edema was more than double for those admissions in which fluid resuscitation was initiated at an outside hospital (OSH) vs. at TCH (13.6 vs. 5.3%, p < 0.001). ConclusionsDecreasing the intended fluid rate during the initial 24 hrs to 2500 mL/m(2)/d and increasing the IV fluid sodium content did not significantly decrease the incidence of adverse outcomes in children with DKA. However, children transferred from an OSH had a higher incidence of suspected clinical cerebral edema. Thus, we need to more readily share our management protocols with the emergency rooms of local referring hospitals to potentially decrease the incidence of suspected clinical cerebral edema and adverse outcomes in children transferred with DKA. C1 [Hsia, Daniel S.; Haymond, Morey W.] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Div Pediat Diabet & Endocrinol, Houston, TX 77030 USA. [Tarai, Sarah G.] Baylor Coll Med, Sch Med, Houston, TX 77030 USA. [Alimi, Amir; Coss-Bu, Jorge A.] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Div Crit Care Med, Houston, TX 77030 USA. [Haymond, Morey W.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, Houston, TX 77030 USA. RP Hsia, DS (reprint author), Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. EM hsiads@pbrc.edu FU NIH [RO1DK 55478, 5T32DK063873-10]; USDA/ARS [6250-5100] FX We would like to thank the staff and physicians of the Texas Children's Hospital Emergency Department, the Sections of Critical Care Medicine and Pediatric Diabetes and Endocrinology for their dedication both day and night, their competency and care for these critically ill children and adolescents with diabetic ketoacidosis. The project was supported by NIH grant RO1DK 55478 (MWH), USDA/ARS 6250-5100 (MWH), and NIH grant 5T32DK063873-10 (DSH). Dr. Hsia is now at the Pennington Biomedical Research Center, Baton Rouge, LA, and Dr. Tarai is now at the University of Indiana, Department of Pediatrics. A portion of the data was presented as a poster at the Pediatric Endocrine Society Annual Meeting in Boston, MA, in May 2012. This work is a publication of the USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX). The contents of this publication do not necessarily reflect the views of policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement from the US Government. NR 28 TC 2 Z9 2 U1 1 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1399-543X EI 1399-5448 J9 PEDIATR DIABETES JI Pediatr. Diabetes PD AUG PY 2015 VL 16 IS 5 BP 338 EP 344 DI 10.1111/pedi.12268 PG 7 WC Endocrinology & Metabolism; Pediatrics SC Endocrinology & Metabolism; Pediatrics GA CM6XD UT WOS:000357833800004 PM 25800410 ER PT J AU Zhang, ML Xue, JJ Zhang, Q Sanderson, SC AF Zhang, Mingli Xue, Juanjuan Zhang, Qiang Sanderson, Stewart C. TI Inferring ancestral distribution area and survival vegetation of Caragana (Fabaceae) in Tertiary SO PLANT SYSTEMATICS AND EVOLUTION LA English DT Article DE Biogeography; Temperate Asia; Arid origin; Adaptive radiation; Spatial evolution; Miocene ID DISPERSAL-VICARIANCE ANALYSIS; TIBETAN PLATEAU; CLIMATE-CHANGE; ASIAN MONSOON; EVOLUTION; DIVERSIFICATION; PHYLOGENY; MIOCENE; UPLIFT; BIOGEOGRAPHY AB Caragana, a leguminous genus mainly restricted to temperate Central and East Asia, occurs in arid, semiarid, and humid belts, and has forest, grassland, and desert ecotypes. Based on the previous molecular phylogenetic tree and dating, biogeographical analyses of extant species area and ecotype were conducted by means of four ancestral optimization approaches: S-DIVA, Lagrange, Mesquite, and BBM. The results indicate the ancestral attributes of Caragana as an arid origin from the Junggar Basin and arid belt of climate and vegetation in the middle Miocene. The ancestral ecotype was most likely adapted to steppe habitats. Uplift and expansion of the Qinghai-Xizang (Tibet) Plateau (QTP) and retreat of the Paratethys Sea are believed to have led to this origin, and also the subsequent diversification and adaptive radiation in the genus. The direction of radiation is suggested in brief to have been from the Central Asian Junggar to East Asia and Tibet. C1 [Zhang, Mingli; Xue, Juanjuan] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. [Zhang, Mingli] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Zhang, Qiang] Chinese Acad Sci, Guangxi Inst Bot, Guangxi Zhuang Autonomous Reg, Guilin 541006, Peoples R China. [Sanderson, Stewart C.] USDA, Shrub Sci Lab, Intermountain Res Stn, Forest Serv, South Jordan, UT 84601 USA. RP Zhang, ML (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. EM zhangml@ibcas.ac.cn FU China National Key Basic Research Program [2012FY111500, 2014CB954201]; Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences FX We are grateful to two anonymous reviewers and editor-in-chief Prof. Marcus Koch for their valuable comments for improving the manuscript. Funding was provided by China National Key Basic Research Program (2012FY111500, 2014CB954201), and Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences. NR 59 TC 0 Z9 0 U1 2 U2 11 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0378-2697 EI 1615-6110 J9 PLANT SYST EVOL JI Plant Syst. Evol. PD AUG PY 2015 VL 301 IS 7 BP 1831 EP 1842 DI 10.1007/s00606-015-1196-z PG 12 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA CM9PK UT WOS:000358041000005 ER PT J AU Ladics, GS Bartholomaeus, A Bregitzer, P Doerrer, NG Gray, A Holzhauser, T Jordan, M Keese, P Kok, E Macdonald, P Parrott, W Privalle, L Raybould, A Rhee, SY Rice, E Romeis, J Vaughn, J Wal, JM Glenn, K AF Ladics, Gregory S. Bartholomaeus, Andrew Bregitzer, Phil Doerrer, Nancy G. Gray, Alan Holzhauser, Thomas Jordan, Mark Keese, Paul Kok, Esther Macdonald, Phil Parrott, Wayne Privalle, Laura Raybould, Alan Rhee, Seung Yon Rice, Elena Romeis, Joerg Vaughn, Justin Wal, Jean-Michel Glenn, Kevin TI Genetic basis and detection of unintended effects in genetically modified crop plants SO TRANSGENIC RESEARCH LA English DT Review DE Unintended effects; GM crop plants; Environmental risk assessment; Allergenicity; Toxicity ID WEED RISK-ASSESSMENT; BIOCHEMICAL PATHWAY DATABASE; ENGINEERED BREEDING STACKS; MULTIPLE FUNGAL PATHOGENS; METABOLIC FLUX ANALYSIS; SOMACLONAL VARIATION; SAFETY ASSESSMENT; INTELLECTUAL DISABILITY; ARABIDOPSIS-THALIANA; TRANSGENIC BARLEY AB In January 2014, an international meeting sponsored by the International Life Sciences Institute/Health and Environmental Sciences Institute and the Canadian Food Inspection Agency titled "Genetic Basis of Unintended Effects in Modified Plants" was held in Ottawa, Canada, bringing together over 75 scientists from academia, government, and the agro-biotech industry. The objectives of the meeting were to explore current knowledge and identify areas requiring further study on unintended effects in plants and to discuss how this information can inform and improve genetically modified (GM) crop risk assessments. The meeting featured presentations on the molecular basis of plant genome variability in general, unintended changes at the molecular and phenotypic levels, and the development and use of hypothesis-driven evaluations of unintended effects in assessing conventional and GM crops. The development and role of emerging "omics" technologies in the assessment of unintended effects was also discussed. Several themes recurred in a number of talks; for example, a common observation was that no system for genetic modification, including conventional methods of plant breeding, is without unintended effects. Another common observation was that "unintended" does not necessarily mean "harmful". This paper summarizes key points from the information presented at the meeting to provide readers with current viewpoints on these topics. C1 [Ladics, Gregory S.] DuPont Pioneer Agr Biotechnol, DuPont Expt Stn, Wilmington, DE 19803 USA. [Bartholomaeus, Andrew] Univ Queensland, Sch Med, Therapeut Res Ctr, Brisbane, Qld 4072, Australia. [Bartholomaeus, Andrew] Univ Canberra, Sch Pharm, Fac Hlth, Canberra, ACT 2601, Australia. [Bregitzer, Phil] ARS, Natl Small Grains Germplasm Res Facil, USDA, Aberdeen, ID 83210 USA. [Doerrer, Nancy G.] ILSI Hlth & Environm Sci Inst, Washington, DC 20005 USA. [Gray, Alan] CEH Wallingford, Ctr Ecol & Hydrol, Crowmarsh Gifford, Wallingford OX10 8BB, Oxon, England. [Holzhauser, Thomas] Paul Ehrlich Inst, Div Allergol, D-63225 Langen, Germany. [Jordan, Mark] Agr & Agri Food Canada, Cereal Res Ctr, Morden, MB R6M 1Y5, Canada. [Keese, Paul] Australian Govt, Off Gene Technol Regulator, Canberra, ACT 2601, Australia. [Kok, Esther] RIKILT Wageningen UR, NL-6700 AE Wageningen, Netherlands. [Macdonald, Phil] Canadian Food Inspect Agcy, Ottawa, ON K1A 0Y9, Canada. [Parrott, Wayne] Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Privalle, Laura] Bayer CropSci, Morrisville, NC 27560 USA. [Raybould, Alan] Syngenta Ltd, Jealotts Hill Int Res Ctr, Bracknell RG42 6EY, Berks, England. [Rhee, Seung Yon] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA. [Rice, Elena] Monsanto Co, Chesterfield, MO 63017 USA. [Romeis, Joerg] Agroscope, Inst Sustainabil Sci ISS, CH-8046 Zurich, Switzerland. [Vaughn, Justin] Univ Georgia, Athens, GA 30602 USA. [Wal, Jean-Michel] AgroParisTech, Dept SVS, F-75231 Paris, France. [Glenn, Kevin] Monsanto Co, St Louis, MO 63167 USA. RP Doerrer, NG (reprint author), ILSI Hlth & Environm Sci Inst, 1156 15th St NW,Suite 200, Washington, DC 20005 USA. EM ndoerrer@hesiglobal.org RI Kok, Esther/B-3919-2013 OI Kok, Esther/0000-0003-1472-6710 NR 103 TC 15 Z9 15 U1 23 U2 163 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD AUG PY 2015 VL 24 IS 4 BP 587 EP 603 DI 10.1007/s11248-015-9867-7 PG 17 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CM9ZQ UT WOS:000358070900001 PM 25716164 ER PT J AU Cingel, A Savic, J Vinterhalter, B Vinterhalter, D Kostic, M Jovanovic, DS Smigocki, A Ninkovic, S AF Cingel, Aleksandar Savic, Jelena Vinterhalter, Branka Vinterhalter, Dragan Kostic, Miroslav Jovanovic, Darka Seslija Smigocki, Ann Ninkovic, Slavica TI y Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor SO TRANSGENIC RESEARCH LA English DT Article DE Solanum tuberosum; Proteinase inhibitor; Oryzacystatin II; Colorado potato beetle; Pest management ID TRANSGENIC TOBACCO PLANTS; MANDUCA-SEXTA LARVAE; PROTEASE INHIBITORS; INSECT RESISTANCE; MOLECULAR-CLONING; MACROSIPHUM-EUPHORBIA; DIGESTIVE PROTEINASES; CYSTEINE PROTEINASES; SOLANUM-TUBEROSUM; METHYL JASMONATE AB Plant proteinase inhibitors (PIs) are attractive tools for crop improvement and their heterologous expression can enhance insect resistance in transgenic plants. PI oryzacystatin II (OCII), isolated from rice, showed potential in controlling pests that utilize cysteine proteinases for protein digestion. To evaluate the applicability of the OCII gene in enhancing plant defence, OCII-transformed potatoes were bioassayed for resistance to Colorado potato beetle (Leptinotarsa decemlineata Say). Feeding on transformed leaves of potato cultivars Desiree and Jelica significantly affected larval growth and development, but did not change mortality rates. During the L2 and L3 developmental stages larvae consumed the OCII-transformed foliage faster as compared to the nontransformed control. Also these larvae reached the prepupal stage (end of L4 stage) 2 days earlier than those fed on control leaves. However, the total amounts of consumed OCII-transformed leaves were up to 23 % lower than of control, and the maximal weights of prepupal larvae were reduced by up to 18 % as compared to larvae fed on nontransformed leaves. The reduction in insect fitness reported in this study in combination with other control measures, could lead to improved CPB resistance management in potato. C1 [Cingel, Aleksandar; Savic, Jelena; Vinterhalter, Branka; Vinterhalter, Dragan; Jovanovic, Darka Seslija; Ninkovic, Slavica] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade, Serbia. [Kostic, Miroslav] Inst Med Plant Res Dr Josif Pancic, Belgrade, Serbia. [Smigocki, Ann] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. RP Cingel, A (reprint author), Univ Belgrade, Inst Biol Res Sinisa Stankovic, Bulevar Despota Stefana 142, Belgrade, Serbia. EM cingel@ibiss.bg.ac.rs FU Ministry of Education, Science and Technological Development of Serbia [ON173015] FX We would like to thank Dr. Lisa Jouanin from Laboratorie de Biologie celulaire, INRA, Versailles, France for kindly provided polyclonal OC antibodies. This research was funded by Ministry of Education, Science and Technological Development of Serbia (Project ON173015). NR 69 TC 2 Z9 2 U1 3 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD AUG PY 2015 VL 24 IS 4 BP 729 EP 740 DI 10.1007/s11248-015-9873-9 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CM9ZQ UT WOS:000358070900011 PM 25820664 ER PT J AU Raybuck, AL Moorman, CE Fritts, SR Greenberg, CH Deperno, CS Simon, DM Warburton, GS AF Raybuck, Amy L. Moorman, Christopher E. Fritts, Sarah R. Greenberg, Cathryn H. Deperno, Christopher S. Simon, Dean M. Warburton, Gordon S. TI Do silvicultural practices to restore oaks affect salamanders in the short term? SO WILDLIFE BIOLOGY LA English DT Article ID SOUTHERN APPALACHIAN SALAMANDERS; MECHANICAL FUEL REDUCTION; ESTIMATING SITE OCCUPANCY; UPLAND HARDWOOD FOREST; BLUE-RIDGE MOUNTAINS; TERRESTRIAL SALAMANDERS; PLETHODON-CINEREUS; SMALL MAMMALS; PRESCRIBED FIRE; TIMBER HARVEST AB Salamanders are an important ecological component of eastern hardwood forests and may be affected by natural or silvicultural disturbances that alter habitat structure and associated microclimate. From May to August in 2008 (pretreatment) and 2011 (post-treatment), we evaluated the response of salamanders to three silvicultural practices designed to promote oak regeneration - prescribed fire, midstory herbicide application and shelterwood harvest - and a control. We trapped salamanders using drift fences with pitfall traps in five replicates of the four treatments. Only the southern gray-cheeked salamander Plethodon metcalfi and the southern Appalachian salamander P. teyahalee were captured in sufficient numbers for robust statistical analysis. We analyzed data for these species using single-species dynamic occupancy models in statistical software program R. We allowed changes in four covariates to influence extinction probability from pre- to post-treatment implementation: 1) percent leaf litter cover; 2) percent understory cover; 3) percent CWD cover; and 4) percent canopy cover. The final combined model set describing extinction probability contained four models with Delta AIC < 2 for P. metcalfi and nine models with Delta AIC < 2, including the null model, for P. teyahalee. For both species, the 95% confidence intervals for model-averaged extinction probability parameter estimates overlapped zero, suggesting none were significant predictors of extinction probability. Absence of short-term salamander response in midstory herbicide and prescribed burn treatments was likely because of minor or transitory changes to forest structure. In shelterwood harvests, any potential effects of reduced canopy and leaf litter cover may have been mitigated by rapid post-treatment vegetation sprouting. Additionally, climatic conditions associated with high elevation sites and high amounts of rainfall in 2011 may have compensated for potential changes to microclimate. Continued monitoring of Plethodon salamanders to assess responses at longer time scales (e.g. > 3 years post-treatment) is warranted. C1 [Raybuck, Amy L.; Moorman, Christopher E.; Fritts, Sarah R.; Deperno, Christopher S.] N Carolina State Univ, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. [Greenberg, Cathryn H.] USDA Forest Serv, Bent Creek Expt Forest, Asheville, NC 28806 USA. [Simon, Dean M.] North Carolina Wildlife Resources Commiss, Lawndale, NC 28090 USA. [Warburton, Gordon S.] North Carolina Wildlife Resources Commiss, Marion, NC 28752 USA. RP Raybuck, AL (reprint author), N Carolina State Univ, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. EM amy_raybuck@live.com FU US Dept of Agriculture Forest Service Southern Research Station (USDA-SRS); Fisheries, Wildlife, and Conservation Biology Program at North Carolina State Univ FX Funding was provided by the US Dept of Agriculture Forest Service Southern Research Station (USDA-SRS) and the Fisheries, Wildlife, and Conservation Biology Program at North Carolina State Univ. This research was initiated by the USDA-SRS Upland Hardwood Ecology and Management Research Work Unit (RWU 4157) in partnership with the USFS Northern Research Station, the North Carolina Wildlife Resources Commission (NCWRC), the Stevenson Land Company, and the Mark Twain National Forest. We thank the many individuals who assisted with drift fence installation and maintenance, data collection and sampling: K. Frick, T. Wait, A. Paoletta, R. Medford, M. Williams, C. Matthews, A. Tschirley, S. Smith, K. Dziwulski, J. Fields, D. Moffitt, J. Golden, S. Ogburn, J. Trammel, J. Sullivan, P. Helm, E. Greene, A. Warner, M. B. Howard and F. Raybuck. We thank T. Roof, J. Adams and site manager T. L. Keyser for providing logistical support and the NCWRC for implementing the treatments. NR 73 TC 3 Z9 3 U1 6 U2 24 PU WILDLIFE BIOLOGY PI RONDE PA C/O JAN BERTELSEN, GRENAAVEJ 14, KALO, DK-8410 RONDE, DENMARK SN 0909-6396 EI 1903-220X J9 WILDLIFE BIOL JI Wildlife Biol. PD AUG PY 2015 VL 21 IS 4 BP 186 EP 194 DI 10.2981/wlb.00076 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CM5KK UT WOS:000357726500001 ER PT J AU Frankel, L Fisher, JO Power, TG Chen, TA Cross, MB Hughes, SO AF Frankel, Leslie Fisher, Jennifer O. Power, Thomas G. Chen, Tzu-An Cross, Matthew B. Hughes, Sheryl O. TI Confirmatory factor analysis of the Feeding Emotions Scale. A measure of parent emotions in the context of feeding SO APPETITE LA English DT Article DE Parent-child dyad; Feeding emotions scale; Measures; Factor analysis; Differential emotions scale ID MOTHERS NEGATIVE AFFECTIVITY; MATERNAL DEPRESSION; FAMILY MEALTIMES; FIT INDEXES; OBESITY; STYLES; CHILDREN; TEMPERAMENT; CHILDHOOD; PRESCHOOL AB Assessing parent affect is important because studies examining the parent-child dyad have shown that parent affect has a profound impact on parent-child interactions and related outcomes. Although some measures that assess general affect during daily lives exist, to date there are only few tools that assess parent affect in the context of feeding. The aim of this study was to develop an instrument to measure parent affect specific to the feeding context and determine its validity and reliability. A brief instrument consisting of 20 items was developed that specifically asks how parents feel during the feeding process. This brief instrument draws on the structure of a well-validated general affect measure. A total of 296 Hispanic and Black Head Start parents of preschoolers completed the Feeding Emotions Scale along with other parent-report measures as part of a larger study designed to better understand feeding interactions during the dinner meal. Confirmatory factor analysis supported a two-factor model with independent subscales of positive affect and negative affect (Cronbach's alphas of 0.85 and 0.84, respectively). Concurrent and convergent construct validity was evaluated by correlating the subscales of the Feeding Emotions Scale with positive emotionality and negative emotionality from the Differential Emotions Scale - a measure of general adult emotions. Concurrent and convergent criterion validity was evaluated by testing mean differences in affect across parent feeding styles using ANOVA. A significant difference was found across maternal weight status for positive feeding affect. The resulting validated measure can be used to assess parent affect in studies of feeding to better understand how interactions during feeding may impact the development of child eating behaviors and possibly weight status. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Frankel, Leslie] Univ Houston, Dept Psychol Hlth & Learning Sci, Houston, TX 77004 USA. [Fisher, Jennifer O.] Temple Univ, Dept Publ Hlth, Philadelphia, PA 19140 USA. [Power, Thomas G.] Washington State Univ, Dept Human Dev, Pullman, WA 99164 USA. [Chen, Tzu-An; Hughes, Sheryl O.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Cross, Matthew B.] Univ Houston, Dept Hlth & Human Performance, Texas Obes Res Ctr, Houston, TX 77204 USA. RP Hughes, SO (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM shughes@bcm.edu OI Cross, Matthew B./0000-0002-9495-0976 FU U. S. Department of Agriculture [2006-55215-16695]; USDA/ARS [6250-51000-053] FX This research was supported by funds from the U. S. Department of Agriculture, Grant No. 2006-55215-16695. This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and had been funded, in part, with federal funds from the USDA/ARS under Cooperative Agreement No. 6250-51000-053. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. NR 39 TC 2 Z9 2 U1 4 U2 12 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD AUG 1 PY 2015 VL 91 BP 107 EP 113 DI 10.1016/j.appet.2015.04.008 PG 7 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA CM2XW UT WOS:000357546600016 PM 25865665 ER PT J AU Chen, TA O'Connor, TM Hughes, SO Beltran, A Baranowski, J Diep, C Baranowski, T AF Chen, Tzu-An O'Connor, Teresia M. Hughes, Sheryl O. Beltran, Alicia Baranowski, Janice Diep, Cassandra Baranowski, Tom TI Vegetable parenting practices scale. Item response modeling analyses SO APPETITE LA English DT Article DE Vegetable; Parenting practices; Multidimensional item response modeling; Differential item functioning ID CHILD FEEDING QUESTIONNAIRE; PRESCHOOL-CHILDREN; EATING PATTERNS; MULTIPLE-CHOICE; FRUIT; ISSUES; DIF; CONSUMPTION; CATEGORIES; STRATEGIES AB Objective: To evaluate the psychometric properties of a vegetable parenting practices scale using multidimensional polytomous item response modeling which enables assessing item fit to latent variables and the distributional characteristics of the items in comparison to the respondents. We also tested for differences in the ways item function (called differential item functioning) across child's gender, ethnicity, age, and household income groups. Method: Parents of 3-5 year old children completed a self-reported vegetable parenting practices scale online. Vegetable parenting practices consisted of 14 effective vegetable parenting practices and 12 ineffective vegetable parenting practices items, each with three subscales (responsiveness, structure, and control). Multidimensional polytomous item response modeling was conducted separately on effective vegetable parenting practices and ineffective vegetable parenting practices. Results: One effective vegetable parenting practice item did not fit the model well in the full sample or across demographic groups, and another was a misfit in differential item functioning analyses across child's gender. Significant differential item functioning was detected across children's age and ethnicity groups, and more among effective vegetable parenting practices than ineffective vegetable parenting practices items. Wright maps showed items only covered parts of the latent trait distribution. The harder- and easier-to-respond ends of the construct were not covered by items for effective vegetable parenting practices and ineffective vegetable parenting practices, respectively. Conclusions: Several effective vegetable parenting practices and ineffective vegetable parenting practices scale items functioned differently on the basis of child's demographic characteristics; therefore, researchers should use these vegetable parenting practices scales with caution. Item response modeling should be incorporated in analyses of parenting practice questionnaires to better assess differences across demographic characteristics. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Chen, Tzu-An; O'Connor, Teresia M.; Hughes, Sheryl O.; Beltran, Alicia; Baranowski, Janice; Diep, Cassandra; Baranowski, Tom] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Chen, TA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St,Rm 4012, Houston, TX 77030 USA. EM anntzuac@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institute of Child Health and Human Development [HD058175]; US Department of Agriculture, Agricultural Research Service [58-6250-6001] FX This research was funded by a grant from the National Institute of Child Health and Human Development (HD058175) and institutional support from the US Department of Agriculture, Agricultural Research Service (Cooperative Agreement no. 58-6250-6001). This manuscript does not represent the views of the USDA. Conflict of interest None declared. NR 78 TC 0 Z9 0 U1 4 U2 11 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD AUG 1 PY 2015 VL 91 BP 190 EP 199 DI 10.1016/j.appet.2015.04.048 PG 10 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA CM2XW UT WOS:000357546600026 PM 25895694 ER PT J AU Fahrner, SJ Lelito, JP Aukema, BH AF Fahrner, Samuel J. Lelito, Jonathan P. Aukema, Brian H. TI The influence of temperature on the flight capacity of emerald ash borer Agrilus planipennis and its parasitoid, Tetrastichus planipennisi: implications to biological control SO BIOCONTROL LA English DT Article DE Coleoptera: Buprestidae; Hymenoptera: Eulophidae; Dispersal; Host-parasitoid interactions ID COLEOPTERA BUPRESTIDAE; HYMENOPTERA EULOPHIDAE; NORTH-AMERICA; RELATIVE-HUMIDITY; NATURAL ENEMIES; COLD TOLERANCE; CONTROL AGENTS; CHINA; PERFORMANCE; DISPERSAL AB The success of classical biological control programs often hinges on an appropriate climatic match between a novel ecosystem and an imported natural enemy. In particular, temperature is the most important factor mediating survival, developmental synchrony, and dispersal of invasive pests and their natural enemies. We used computer-monitored flight mills to investigate the roles of temperature and humidity in the flight distance, flight speed, number of flights, and post-flight mortality of emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) and its parasitoid Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) in laboratory settings. Flight distance had a concave, parabolic relationship with temperature for both insects. Maximum flight distances in 24 h occurred at 27.9 A degrees C (1.13 km) for emerald ash borer and 26.5 A degrees C (1.81 km) for T. planipennisi. Flight speed and post-flight mortality increased with temperature for both insects. Relative humidity was inversely related to flight speeds for emerald ash borer, but positively correlated with the number of flights and post-flight survival by T. planipennisi. We integrated the relationship between flight distance and temperature for both insects with landscape temperatures and flight periods for insects at ten locations across the continental USA to develop a flight index. The flight index offers a relative scale of flight capacity across locations based solely on temperature. We found that the flight index for Detroit was intermediate to low compared to other locations, with the smallest and largest flight indices projected in Minneapolis and Seattle for emerald ash borer and Seattle and Atlanta for T. planipennisi. The implications of results for host-parasitoid dispersal patterns and release protocols for distributing T. planipennisi are discussed. C1 [Fahrner, Samuel J.; Aukema, Brian H.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Lelito, Jonathan P.] Emerald Ash Borer Biol Control, USDA APHIS PPQ, Brighton, MI 48116 USA. RP Fahrner, SJ (reprint author), Univ Minnesota, Dept Entomol, 1980 Folwell Ave, St Paul, MN 55108 USA. EM fahr0051@umn.edu; jplelito@gmail.com; BrianAukema@umn.edu FU Legislative-Citizen Commission on Minnesota Resources; McKnight Land-Grant Professorship funds; College of Food, Agricultural, and Natural Resource Sciences at the University of Minnesota FX We thank Dr. George E. Heimpel (University of Minnesota, USA) for advice in development of the flight mill and Jonathan Aukema, John Vanstone, and Jeff Cassidy (Western University, London, Ontario, Canada) for their technical expertise. Drs. Robert C. Venette (USDA Forest Service, Northern Research Station), handling editor Stefano Colazza, editor in chief Eric Wajnberg, and two anonymous reviewers provided helpful comments that improved earlier drafts of this manuscript. Funding was provided by the Legislative-Citizen Commission on Minnesota Resources, McKnight Land-Grant Professorship funds to BHA, and the College of Food, Agricultural, and Natural Resource Sciences at the University of Minnesota. NR 55 TC 2 Z9 2 U1 8 U2 35 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 EI 1573-8248 J9 BIOCONTROL JI Biocontrol PD AUG PY 2015 VL 60 IS 4 BP 437 EP 449 DI 10.1007/s10526-015-9657-4 PG 13 WC Entomology SC Entomology GA CM1ZD UT WOS:000357478300001 ER PT J AU Rezende, JM Zanardo, ABR Lopes, MD Delalibera, I Rehner, SA AF Rezende, Janayne Maria Riguetti Zanardo, Ana Beatriz Lopes, Mariana da Silva Delalibera, Italo, Jr. Rehner, Stephen A. TI Phylogenetic diversity of Brazilian Metarhizium associated with sugarcane agriculture SO BIOCONTROL LA English DT Article DE Hypocreales; Clavicipitaceae; Biological control; Entomopathogenic fungi; Molecular characterization; Phylogeny ID ENTOMOPATHOGENIC FUNGI; ANISOPLIAE; SPP.; SOIL; AGROECOSYSTEM; INSECTS; COMPLEX AB Biological control of spittlebugs with Metarhizium (Hypocreales: Clavicipitaceae) in Brazilian sugarcane is an example of effective pest management. However, little is known about the richness, distribution and ecology of Metarhizium species in Brazilian agroecosystems. We investigated Metarhizium diversity within a collection of 96 Brazilian isolates from spittlebugs and other insects, strains used for spittlebug control and soil isolates from sugarcane and other field crops and pristine habitats. A multilocus phylogenetic analysis of 5'-TEF and nuclear intergenic loci MzFG543igs and MzIGS3 yielded robust support for current species limits of the two most abundant taxa, Metarhizium anisopliae and Metarhizium robertsii, and the resolution of two lineages that lie beyond currently recognized species limits in this complex. With a single exception, all isolates from insects belong to a single subclade of M. anisopliae. These data will serve as resources about Metarhizium biodiversity for insect biological control initiatives in South America. C1 [Rezende, Janayne Maria; Riguetti Zanardo, Ana Beatriz; Lopes, Mariana da Silva; Delalibera, Italo, Jr.] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Entomol & Acarol, BR-13418900 Sao Paulo, Brazil. [Rehner, Stephen A.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Rezende, JM (reprint author), Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Entomol & Acarol, Av Padua Dias 11,POB 9, BR-13418900 Sao Paulo, Brazil. EM janaynerezende@gmail.com RI Riguetti Zanardo, Ana Beatriz /L-5695-2015 FU National Council for the Improvement of Higher Education (CAPES) in Brazil; National Council for Scientific and Technological Development (CNPq) in USA; CNPq [47/2010, 563233/2010-9]; FAPESP [2010/52342-4] FX The first author is grateful to the National Council for the Improvement of Higher Education (CAPES) and National Council for Scientific and Technological Development (CNPq) for research scholarship awards that supported this project in Brazil and USA, respectively. This work was partly supported by the research project SISBIOTA BRASIL (FAPs No 47/2010) from CNPq (Grant No 563233/2010-9) and FAPESP (Grant No 2010/52342-4). We thank Veronica Martins for laboratory assistance. The authors declare no conflict of interest. The mention of trade products or company or firm names does not imply that the U.S. Department of Agriculture recommends them over similar products or companies not mentioned. NR 33 TC 2 Z9 3 U1 5 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 EI 1573-8248 J9 BIOCONTROL JI Biocontrol PD AUG PY 2015 VL 60 IS 4 BP 495 EP 505 DI 10.1007/s10526-015-9656-5 PG 11 WC Entomology SC Entomology GA CM1ZD UT WOS:000357478300006 ER PT J AU Ulrich, KR Feldlaufer, MF Kramer, M St Leger, RJ AF Ulrich, Kevin R. Feldlaufer, Mark F. Kramer, Matthew St Leger, Raymond J. TI Inhibition of the entomopathogenic fungus Metarhizium anisopliae sensu lato in vitro by the bed bug defensive secretions (E)-2-hexenal and (E)-2-octenal SO BIOCONTROL LA English DT Article DE Cimex lectularius (Hemiptera: Cimicidae); Aldehydes; Antifungal activity; Volatiles; Defensive chemistry; Glandular secretions ID BIOLOGICAL-CONTROL AGENTS; CIMEX-LECTULARIUS L; ALIPHATIC-ALDEHYDES; BEAUVERIA-BASSIANA; ALARM PHEROMONE; LARVAE; IDENTIFICATION; AGGREGATION; ARTHROPODS; MECHANISMS AB The two major aldehydes (E)-2-hexenal and (E)-2-octenal emitted as defensive secretions by bed bugs Cimex lectularius L. (Hemiptera: Cimicidae), inhibit the in vitro growth of an isolate of Metarhizium anisopliae sensu lato (s.l.) (Metsch.) Sokorin (Hypocreales: Clavicipitaceae) (ARSEF 1548). These chemicals inhibit fungal growth by direct contact and via indirect exposure ("fumigation"). Fumigation with (E)-2-octenal for as little as 0.5 h was sufficient to inhibit all fungal growth. Bed bugs placed on filter paper treated with an isolate of M. anisopliae s.l. conidia in the absence of (E)-2-octenal exhibited 99 % mortality after one week. However, bed bugs placed on fungal-treated filter paper and exposed to (E)-2-octenal at 1 h experienced 10 % mortality. The inhibition of fungal growth by bed bug aldehydes is discussed in the context of other biotic and abiotic barriers to infection. C1 [Ulrich, Kevin R.; St Leger, Raymond J.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Feldlaufer, Mark F.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Kramer, Matthew] ARS, Biometr Consulting Serv, USDA, Beltsville, MD 20705 USA. RP Ulrich, KR (reprint author), Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. EM kru@umd.edu FU University of Maryland's Department of Entomology's Gahan Fellowship FX We thank Barbara Thorne (University of Maryland, College Park, USA) for guidance and reviewing the manuscript. We also thank members of the Armed Forces Pest Management Board (Silver Spring MD, USA), and personnel at the Walter Reed National Military Medical Center (Bethesda MD, USA) for their help in obtaining the blood products used to maintain insect colonies. This project was funded in part by the University of Maryland's Department of Entomology's Gahan Fellowship. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employee. NR 47 TC 1 Z9 2 U1 4 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 EI 1573-8248 J9 BIOCONTROL JI Biocontrol PD AUG PY 2015 VL 60 IS 4 BP 517 EP 526 DI 10.1007/s10526-015-9667-2 PG 10 WC Entomology SC Entomology GA CM1ZD UT WOS:000357478300008 ER PT J AU Crawford, JN Mensing, SA Lake, FK Zimmerman, SR AF Crawford, Jeffrey N. Mensing, Scott A. Lake, Frank K. Zimmerman, Susan R. H. TI Late Holocene fire and vegetation reconstruction from the western Klamath Mountains, California, USA: A multi-disciplinary approach for examining potential human land-use impacts SO HOLOCENE LA English DT Review DE anthropogenic impacts; California; fire history; forest structure; Klamath Mountains; vegetation change ID TREE-RING RECORDS; POSTGLACIAL VEGETATION; SISKIYOU MOUNTAINS; NORTHWESTERN CALIFORNIA; NORTHERN CALIFORNIA; CLIMATE-CHANGE; UNITED-STATES; HISTORY; CHARCOAL; FORESTS AB The influence of Native American land-use practices on vegetation composition and structure has long been a subject of significant debate. This is particularly true in portions of the western United States where tribal hunter-gatherers did not use agriculture to meet subsistence and other cultural needs. Climate has been viewed as the dominant determinant of vegetation structure and composition change over time, but ethnographic and anthropological evidence suggests that Native American land-use practices (particularly through the use of fire) had significant landscape effects on vegetation. However, it is difficult to distinguish climatically driven vegetation change from human-caused vegetation change using traditional paleoecological methods. To address this problem, we use a multidisciplinary methodology that incorporates paleoecology with local ethnographic and archaeological information at two lake sites in northwestern California. We show that anthropogenic impacts can be distinguished at our Fish Lake site during the cool and wet Little Ice Age', when we have evidence for open-forest or shade-intolerant vegetation, fostered for subsistence and cultural purposes, rather than the closed-forest or shade-tolerant vegetation expected due to the climatic shift. We also see a strong anthropogenic influence on modern vegetation at both sites following European settlement, decline in tribal use, and subsequent fire exclusion. These results demonstrate that Native American influences on vegetation structure and composition can be distinguished using methods that take into account both physical and cultural aspects of the landscape. They also begin to determine the scale at which western forests were influenced by Native American land-use practices and how modern forests of northwestern California are not solely products of climate alone. C1 [Crawford, Jeffrey N.; Mensing, Scott A.] Univ Nevada, Dept Geog, Mackay Sci MS 154, Reno, NV 89557 USA. [Lake, Frank K.] Pacif Southwest Res Stn, US Forest Serv, Albany, CA USA. [Zimmerman, Susan R. H.] Lawrence Livermore Natl Lab, Ctr Accelerator Mass Spectrometry, Livermore, CA USA. RP Mensing, SA (reprint author), Univ Nevada, Dept Geog, Mackay Sci MS 154, 201 Mackay Sci Hall, Reno, NV 89557 USA. EM smensing@unr.edu FU National Science Foundation [0926732, 0964261]; Lawrence Livermore National Laboratory [09ERI003]; US Department of Energy by Lawrence Livermore National Laboratory [DE-AC52-07NA27344] FX This research was supported by grants from the National Science Foundation (#0926732 and #0964261) and Lawrence Livermore National Laboratory Grant #09ERI003. This work was performed in part under the auspices of the US Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344. NR 101 TC 3 Z9 3 U1 1 U2 27 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0959-6836 EI 1477-0911 J9 HOLOCENE JI Holocene PD AUG PY 2015 VL 25 IS 8 BP 1341 EP 1357 DI 10.1177/0959683615584205 PG 17 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CM2PT UT WOS:000357524500012 ER PT J AU Brunton, J Steele, S Miller, C Lovullo, E Taft-Benz, S Kawula, T AF Brunton, J. Steele, S. Miller, C. Lovullo, E. Taft-Benz, S. Kawula, T. TI Identifying Francisella tularensis Genes Required for Growth in Host Cells SO INFECTION AND IMMUNITY LA English DT Article ID PATHOGENICITY ISLAND; INTRACELLULAR SURVIVAL; PHAGOSOMAL ESCAPE; TULAREMIA VACCINE; SCHU S4; MACROPHAGES; LVS; INHALATION; SECRETION; APOPTOSIS AB Francisella tularensis is a highly virulent Gram-negative intracellular pathogen capable of infecting a vast diversity of hosts, ranging from amoebae to humans. A hallmark of F. tularensis virulence is its ability to quickly grow to high densities within a diverse set of host cells, including, but not limited to, macrophages and epithelial cells. We developed a luminescence reporter system to facilitate a large-scale transposon mutagenesis screen to identify genes required for growth in macrophage and epithelial cell lines. We screened 7,454 individual mutants, 269 of which exhibited reduced intracellular growth. Transposon insertions in the 269 growth-defective strains mapped to 68 different genes. FTT_0924, a gene of unknown function but highly conserved among Francisella species, was identified in this screen to be defective for intracellular growth within both macrophage and epithelial cell lines. FTT_0924 was required for full Schu S4 virulence in a murine pulmonary infection model. The Delta FTT_0924 mutant bacterial membrane is permeable when replicating in hypotonic solution and within macrophages, resulting in strongly reduced viability. The permeability and reduced viability were rescued when the mutant was grown in a hypertonic solution, indicating that FTT_0924 is required for resisting osmotic stress. The Delta FTT_0924 mutant was also significantly more sensitive to beta-lactam antibiotics than Schu S4. Taken together, the data strongly suggest that FTT_0924 is required for maintaining peptidoglycan integrity and virulence. C1 [Brunton, J.; Steele, S.; Taft-Benz, S.; Kawula, T.] Univ N Carolina, Sch Med, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA. [Miller, C.] Washington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Lovullo, E.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. RP Kawula, T (reprint author), Univ N Carolina, Sch Med, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA. EM kawula@med.unc.edu FU NIH [AI082870] FX This work was supported by NIH grant AI082870 (T.K.). NR 34 TC 0 Z9 0 U1 1 U2 11 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 2015 VL 83 IS 8 BP 3015 EP 3025 DI 10.1128/IAI.00004-15 PG 11 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CM3XI UT WOS:000357618300002 PM 25987704 ER PT J AU Lone, AG Atci, E Renslow, R Beyenal, H Noh, S Fransson, B Abu-Lail, N Park, JJ Gang, DR Call, DR AF Lone, Abdul G. Atci, Erhan Renslow, Ryan Beyenal, Haluk Noh, Susan Fransson, Boel Abu-Lail, Nehal Park, Jeong-Jin Gang, David R. Call, Douglas R. TI Colonization of Epidermal Tissue by Staphylococcus aureus Produces Localized Hypoxia and Stimulates Secretion of Antioxidant and Caspase-14 Proteins SO INFECTION AND IMMUNITY LA English DT Article ID HUMAN SKIN; HUMAN KERATINOCYTES; ATOPIC-DERMATITIS; IN-VITRO; INFECTIONS; OXYGEN; SURFACE; ACTIVATION; RESISTANCE; DIFFUSION AB A partial-thickness epidermal explant model was colonized with green fluorescent protein (GFP)-expressing Staphylococcus aureus, and the pattern of S. aureus biofilm growth was characterized using electron and confocal laser scanning microscopy. The oxygen concentration in explants was quantified using microelectrodes. The relative effective diffusivity and porosity of the epidermis were determined using magnetic resonance imaging, while hydrogen peroxide (H2O2) concentration in explant media was measured by using microelectrodes. Secreted proteins were identified and quantified using elevated-energy mass spectrometry (MSE). S. aureus biofilm grows predominantly in lipid-rich areas around hair follicles and associated skin folds. Dissolved oxygen was selectively depleted (2- to 3-fold) in these locations, but the relative effective diffusivity and porosity did not change between colonized and control epidermis. Histological analysis revealed keratinocyte damage across all the layers of colonized epidermis after 4 days of culture. The colonized explants released significantly (P<0.01) more antioxidant proteins of both epidermal and S. aureus origin, consistent with elevated H2O2 concentrations found in the media from the colonized explants (P<0.001). Caspase-14 was also elevated significantly in the media from the colonized explants. While H2O2 induces primary keratinocyte differentiation, caspase-14 is required for terminal keratinocyte differentiation and desquamation. These results are consistent with a localized biological impact from S. aureus in response to colonization of the skin surface. C1 [Lone, Abdul G.; Call, Douglas R.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Atci, Erhan; Beyenal, Haluk; Abu-Lail, Nehal] Washington State Univ, Sch Chem Engn & Bioengn, Pullman, WA 99164 USA. [Renslow, Ryan] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA. [Noh, Susan] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Noh, Susan; Call, Douglas R.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Fransson, Boel] Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA. [Park, Jeong-Jin; Gang, David R.] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA. RP Call, DR (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM drcall@wsu.edu OI Call, Douglas/0000-0001-6791-055X FU U.S. Department of Defense [DM110308]; Agricultural Animal Health Program, Washington State University; Washington State Agricultural Research Center, Pullman, WA; National Science Foundation [DBI-1229749]; Linus Pauling Distinguished Postdoctoral Fellowship at Pacific Northwest National Laboratory; Department of Energy's Office of Biological and Environmental Research FX This research was supported in part by a grant (DM110308) from the U.S. Department of Defense and by the Agricultural Animal Health Program, Washington State University, and the Washington State Agricultural Research Center, Pullman, WA. Mass spectrometric analysis was performed on an instrument acquired through a Major Research Instrumentation grant (DBI-1229749) from National Science Foundation to D.R.G. Ryan Renslow was partially supported by a Linus Pauling Distinguished Postdoctoral Fellowship at Pacific Northwest National Laboratory.; We thank Niles Donegan, the Geisel School of Medicine, Dartmouth, for his kind gift of GFP-labeled S. aureus. We also thank Carla Schubiger and Lisa Orfe for their help in the laboratory and Tom Tevlin and Daniel Broeckel of Garfield Meats (Garfield, WA) and Sam Hunt and Jake Brunton of C&L Locker (Moscow, ID) for their generous gift of pig ears. All NMR experiments were performed at the Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility sponsored by the Department of Energy's Office of Biological and Environmental Research and located at the Pacific Northwest National Laboratory. NR 63 TC 1 Z9 1 U1 1 U2 17 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 2015 VL 83 IS 8 BP 3026 EP 3034 DI 10.1128/IAI.00175-15 PG 9 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CM3XI UT WOS:000357618300003 PM 25987705 ER PT J AU Wang, XF Su, HN Huang, L Deng, XL Chen, JC Zhou, CY Li, ZG AF Wang, Xuefeng Su, Huanan Huang, Li Deng, Xiaoling Chen, Jianchi Zhou, Changyong Li, Zhongan TI Identification of a Novel 1033-Nucleotide Deletion Polymorphism in the Prophage Region of "Candidatus Liberibacter asiaticus': Potential Applications for Bacterial Epidemiology SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE "Candidatus Liberibacter asiaticus'; epidemiology; genetic diversity; prophage ID GREENING DISEASE; TANDEM REPEATS; CITRUS; ALIGNMENT; SEQUENCE; GENOME; LOCUS AB The prophage/phage region in the genome of Candidatus Liberibacter asiaticus', an alpha-proteobacterium associated with citrus Huanglongbing, included many valuable loci for genetic diversity studies. Previously, a mosaic genomic region (CLIBASIA_05640 to CLIBASIA_05650) was characterized, and this revealed inter- and intracontinental variations of Ca. L. asiaticus'. In this study, 267 Ca. L. asiaticus' isolates collected from eight provinces in China were analysed with a primer set flanking the same mosaic region plus downstream sequence. While most amplicon sizes ranged from 1400 to 2000bp, an amplicon of 550bp (S550) was found in 14 samples collected from south-western China. Sequence analyses showed that S550 was the result of a 1033bp deletion which included the previously known mosaic region. The genetic nature of the deletion event remains unknown. The regional restriction of S550 suggests that the Ca. L. asiaticus' population from south-western China is different from those in eastern China. The small and easy-to-detect S550 amplicon could serve as a molecular marker for Ca. L. asiaticus' epidemiology. C1 [Wang, Xuefeng; Su, Huanan; Huang, Li; Zhou, Changyong; Li, Zhongan] Southwest Univ, Citrus Res Inst, Natl Citrus Engn Res Ctr, Chongqing 400712, Peoples R China. [Deng, Xiaoling] South China Agr Univ, Citrus Huanglongbing Res Ctr, Guangzhou 510642, Guangdong, Peoples R China. [Chen, Jianchi] ARS, USDA, Parlier, CA 93648 USA. RP Wang, XF (reprint author), Southwest Univ, Citrus Res Inst, Natl Citrus Engn Res Ctr, Chongqing 400712, Peoples R China. EM wangxuefeng@cric.cn; zhongan@cric.cn FU Special Fund for Agro-scientific Research in the Public Interest [201003067-02]; Natural Science Foundation Project CQ CSTC [cstc2012jjA80025]; Chongqing Key Laboratory of Citrus [CKLC201108]; Fundamental Research Funds for the Central Universities [XDJK2014A001] FX This work was partially supported by Special Fund for Agro-scientific Research in the Public Interest (201003067-02), Natural Science Foundation Project CQ CSTC (cstc2012jjA80025), Chongqing Key Laboratory of Citrus (CKLC201108) and Fundamental Research Funds for the Central Universities (XDJK2014A001). We thank E. Civerolo in reviewing our manuscript. NR 19 TC 1 Z9 1 U1 5 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD AUG PY 2015 VL 163 IS 7-8 BP 681 EP 685 DI 10.1111/jph.12307 PG 5 WC Plant Sciences SC Plant Sciences GA CM1UI UT WOS:000357465500022 ER PT J AU Frank, MH Edwards, MB Schultz, ER McKain, MR Fei, ZJ Sorensen, I Rose, JKC Scanlon, MJ AF Frank, Margaret H. Edwards, Molly B. Schultz, Eric R. McKain, Michael R. Fei, Zhangjun Sorensen, Iben Rose, Jocelyn K. C. Scanlon, Michael J. TI Dissecting the molecular signatures of apical cell-type shoot meristems from two ancient land plant lineages SO NEW PHYTOLOGIST LA English DT Article DE apical cell (AC); Equisetum arvense; seedless vascular plants; Selaginella moellendorfii; shoot apical meristem (SAM) ID KNOX HOMEOBOX GENES; CLASS IIIHD-ZIP; SELAGINELLA-MARTENSII; VASCULAR PLANTS; VECTOR REMOVAL; ARABIDOPSIS; EVOLUTION; EXPRESSION; PATTERN; GROWTH AB Shoot apical meristem (SAM) structure varies markedly within the land plants. The SAMs of many seedless vascular plants contain a conspicuous inverted, pyramidal cell called the apical cell (AC), which is unidentified in angiosperms. In this study, we use transcriptomic sequencing with precise laser microdissections of meristem subdomains to define the molecular signatures of anatomically distinct zones from the AC-type SAMs of a lycophyte (Selaginella moellendorffii) and a monilophyte (Equisetum arvense). The two model species for this study represent vascular plant lineages that diverged >400million yr ago. Our data comprise comprehensive molecular signatures for the distinct subdomains within AC-type SAMs, an anatomical anomaly whose functional significance has been debated in the botanical literature for over two centuries. Moreover, our data provide molecular support for distinct gene expression programs between the AC-type SAMs of Selaginella and Equisetum, as compared with the SAM transcriptome of the angiosperm maize. The results are discussed in light of the functional significance and evolutionary success of the AC-type SAM within the embryophytes. C1 [Frank, Margaret H.; Edwards, Molly B.; Schultz, Eric R.; Sorensen, Iben; Rose, Jocelyn K. C.; Scanlon, Michael J.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [McKain, Michael R.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Scanlon, MJ (reprint author), Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. EM mjs298@cornell.edu FU National Science Foundation [IOS-1146733] FX We thank K. Niklas for taxonomic identification of E. arvense samples, and A. Stalter for preparation of voucher specimens. We are also grateful to the reviewers for their thoughtful and constructive feedback. This work was supported by National Science Foundation grant IOS-1146733. Sequence files have been uploaded to the NCBI SRA database under the following accession numbers: Selaginella (SRX513905), Equisetum (SRX514722), and maize (SRX514742). The annotated E. arvense transcriptome is available for download from the Dataverse website:http://thedata.harvard.edu/dvn/dv/EqTransc. NR 89 TC 8 Z9 8 U1 6 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD AUG PY 2015 VL 207 IS 3 BP 893 EP 904 DI 10.1111/nph.13407 PG 12 WC Plant Sciences SC Plant Sciences GA CM6TZ UT WOS:000357824400038 PM 25900772 ER PT J AU Guo, XL Chronis, D De La Torre, CM Smeda, J Wang, XH Mitchum, MG AF Guo, Xiaoli Chronis, Demosthenis De La Torre, Carola M. Smeda, John Wang, Xiaohong Mitchum, Melissa G. TI Enhanced resistance to soybean cyst nematode Heterodera glycines in transgenic soybean by silencing putative CLE receptors SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE CLAVATA; CLE; cyst nematode; Heterodera; soybean; transgenic ID PLANT-PARASITIC NEMATODE; STEM-CELL FATE; MEDIATES SYSTEMIC REGULATION; GLOBODERA-ROSTOCHIENSIS; ARABIDOPSIS-THALIANA; EFFECTOR PROTEINS; ORGAN DEVELOPMENT; LOTUS-JAPONICUS; KINASE; GENES AB CLE peptides are small extracellular proteins important in regulating plant meristematic activity through the CLE-receptor kinase-WOX signalling module. Stem cell pools in the SAM (shoot apical meristem), RAM (root apical meristem) and vascular cambium are controlled by CLE signalling pathways. Interestingly, plant-parasitic cyst nematodes secrete CLE-like effector proteins, which act as ligand mimics of plant CLE peptides and are required for successful parasitism. Recently, we demonstrated that Arabidopsis CLE receptors CLAVATA1 (CLV1), the CLAVATA2 (CLV2)/CORYNE (CRN) heterodimer receptor complex and RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2), which transmit the CLV3 signal in the SAM, are required for perception of beet cyst nematode Heterodera schachtii CLEs. Reduction in nematode infection was observed in clv1, clv2, crn, rpk2 and combined double and triple mutants. In an effort to develop nematode resistance in an agriculturally important crop, orthologues of Arabidopsis receptors including CLV1, CLV2, CRN and RPK2 were identified from soybean, a host for the soybean cyst nematode Heterodera glycines. For each of the receptors, there are at least two paralogues in the soybean genome. Localization studies showed that most receptors are expressed in the root, but vary in their level of expression and spatial expression patterns. Expression in nematode-induced feeding cells was also confirmed. In vitro direct binding of the soybean receptors with the HgCLE peptide was analysed. Knock-down of the receptors in soybean hairy roots showed enhanced resistance to SCN. Our findings suggest that targeted disruption of nematode CLE signalling may be a potential means to engineer nematode resistance in crop plants. C1 [Guo, Xiaoli; De La Torre, Carola M.; Smeda, John; Mitchum, Melissa G.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Guo, Xiaoli; De La Torre, Carola M.; Smeda, John; Mitchum, Melissa G.] Univ Missouri, Bond Life Sci Ctr, Columbia, MO USA. [Chronis, Demosthenis; Wang, Xiaohong] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Wang, Xiaohong] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. RP Mitchum, MG (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM goellnerm@missouri.edu FU USDA-AFRI; MU-HHMI C3 Undergraduate Research Fellowship; Daniel F. Millikan Endowment FX We gratefully acknowledge funding by grants from USDA-AFRI to M.G.M and X.W., a MU-HHMI C3 Undergraduate Research Fellowship to J.S., and funding from the Daniel F. Millikan Endowment to C.C. We thank Robert Heinz for nematode population maintenance. NR 62 TC 7 Z9 8 U1 6 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD AUG PY 2015 VL 13 IS 6 BP 801 EP 810 DI 10.1111/pbi.12313 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CM3TI UT WOS:000357606800007 PM 25581705 ER PT J AU Knipfer, T Eustis, A Brodersen, C Walker, AM McElrone, AJ AF Knipfer, Thorsten Eustis, Ashley Brodersen, Craig Walker, Andrew M. McElrone, Andrew J. TI Grapevine species from varied native habitats exhibit differences in embolism formation/repair associated with leaf gas exchange and root pressure SO PLANT CELL AND ENVIRONMENT LA English DT Article DE cavitation; drought; recovery; Vitis; vulnerability; water stress; xylem ID RESOLUTION COMPUTED-TOMOGRAPHY; STRESS-INDUCED CAVITATION; IN-VIVO VISUALIZATIONS; XYLEM CAVITATION; VITIS-VINIFERA; WATER-STRESS; NONIRRIGATED CONDITIONS; VULNERABILITY; REPAIR; DROUGHT AB Drought induces xylem embolism formation, but grapevines can refill non-functional vessels to restore transport capacity. It is unknown whether vulnerability to embolism formation and ability to repair differ among grapevine species. We analysed in vivo embolism formation and repair using x-ray computed microtomography in three wild grapevine species from varied native habitats (Vitis riparia, V.arizonica, V.champinii) and related responses to measurements of leaf gas exchange and root pressure. Vulnerability to embolism formation was greatest in V.riparia, intermediate in V.arizonica and lowest in V.champinii. After re-watering, embolism repair was rapid and pronounced in V.riparia and V.arizonica, but limited or negligible in V.champinii even after numerous days. Similarly, root pressure measured after re-watering was positively correlated with drought stress severity for V.riparia and V.arizonica (species exhibiting embolism repair) but not for V.champinii. Drought-induced reductions in transpiration were greatest for V.riparia and least in V.champinii. Recovery of transpiration after re-watering was delayed for all species, but was greatest for V.champinii and most rapid in V.arizonica. These species exhibit varied responses to drought stress that involve maintenance/recovery of xylem transport capacity coordinated with root pressure and gas exchange responses. Grapevines can refill xylem vessels that have been embolized by drought, but it is unknown whether vulnerability to embolism formation and ability to repair differ among grapevine species. We analyzed in vivo embolism formation and repair using x-ray microtomography in three wild grapevine species from varied native habitats that represent important germplasm for commercial rootstocks. The species exhibited varied responses to drought stress that involve maintenance/recovery of xylem transport capacity coordinated with root pressure and gas exchange responses. Our findings provide new potential criteria for screening germplasm collections for drought stress resistance and insights into the mechanisms of embolism repair in these species. C1 [Knipfer, Thorsten; Eustis, Ashley; Walker, Andrew M.; McElrone, Andrew J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Brodersen, Craig] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [McElrone, Andrew J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP McElrone, AJ (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM ajmcelrone@ucdavis.edu RI Brodersen, Craig/J-1112-2016 OI Brodersen, Craig/0000-0002-0924-2570 FU NIFA SCRI grant; American Vineyard Foundation; USDA-ARS CRIS [5306-21220-004-00]; Office of Science, Office of Basic Energy Sciences, of the US Department of Energy [DE-AC02-05CH11231] FX This work was supported by a NIFA SCRI grant and funding from the American Vineyard Foundation to A.J.M. and USDA-ARS CRIS funding (Research Project #5306-21220-004-00). The authors kindly thank D. Parkinson and A. MacDowell for their assistance at the Lawrence Berkeley National Laboratory Advanced Light Source Beamline 8.3.2 microtomography facility. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the US Department of Energy under contract no. DE-AC02-05CH11231. Thanks to A. Fanton Borges, S. Castorani, G.A. Gambetta, Z. Nasafi, C. Osorio and A. Zedan for help with plant preparation and/or image collection and processing. NR 57 TC 18 Z9 19 U1 3 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD AUG PY 2015 VL 38 IS 8 BP 1503 EP 1513 DI 10.1111/pce.12497 PG 11 WC Plant Sciences SC Plant Sciences GA CM3UB UT WOS:000357609100007 PM 25495925 ER PT J AU Ehlenfeldt, MK Camp, MJ Wang, SY AF Ehlenfeldt, Mark K. Camp, Mary J. Wang, Shiow Y. TI alpha-Glucosidase inhibitory activity and antioxidant capacity in the peel and pulp of mixed-species blueberry hybrids SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE anthocyanins; highbush; phenolics; rabbiteye; Vaccinium ID AMYLASE; OXYGEN; ASSAY AB Inhibition of alpha-glucosidase activity is considered an effective means for controlling diabetes by regulating glucose uptake, and blueberries have been shown to possess high levels of inhibitory activity. In the present study, we examined the variations in alpha-glucosidase inhibition, phenolic and anthocyanin levels, and antioxidant capacity in the peel and pulp of 16 mixed-species rabbiteye hybrids (Vaccinium ashei ReadexVaccinium spp.), one rabbiteye cultivar (V. ashei) and two highbush hybrids (Vaccinium corymbosum). Peel tissue had, on average, about four times higher levels of alpha-glucosidase inhibitory activity than pulp, and exhibited significantly higher levels of all other measured activities, even though the peel comprised only a small portion of the fruit. Significant variations in the levels of antioxidant activity were observed; however, no consistent differences were observed between the hybrids with various species composition. Significant positive correlations (r >= 0.84) were found among -glucosidase inhibitory activity, total anthocyanin (TA) and phenolic levels, and scavenging activity against ROO., center dot OH, O-1(2) and H2O2 radicals in the extracts from the peel and pulp. There was a high correlation observed between alpha-glucosidase inhibitory activity levels and ROO.(ORAC)(peel) (r=0.95). A similarly high correlation with TA(peel) (r=0.93) suggests that TA would be a suitable assay proxy if a broader genotypic evaluation of blueberry genotypes is desired. C1 [Ehlenfeldt, Mark K.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ 08019 USA. [Camp, Mary J.] USDA ARS, Beltsville Agr Res Ctr BARC, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Wang, Shiow Y.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, BARC, Beltsville, MD 20705 USA. [Wang, Shiow Y.] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. RP Ehlenfeldt, MK (reprint author), USDA ARS, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ 08019 USA. EM mark.ehlenfeldt@ars.usda.gov NR 16 TC 0 Z9 0 U1 6 U2 21 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 EI 1479-263X J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD AUG PY 2015 VL 13 IS 2 BP 190 EP 194 DI 10.1017/S1479262114000690 PG 5 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA CM6TD UT WOS:000357822000015 ER PT J AU Manici, LM Kelderer, M Caputo, F Mazzola, M AF Manici, L. M. Kelderer, M. Caputo, F. Mazzola, M. TI Auxin-mediated relationships between apple plants and root inhabiting fungi: impact on root pathogens and potentialities of growth-promoting populations SO PLANT PATHOLOGY LA English DT Article DE binucleate Rhizoctonia sp; Cylindrocarpon-like fungi; Fusarium spp; indole-3-acetic acid (IAA); replant disease; rooting induction ID REPLANT DISEASE; GIBBERELLIN BIOSYNTHESIS; FUSARIUM; SUSCEPTIBILITY; IDENTIFICATION; SEQUESTRATION; ENDOPHYTES; BACTERIAL; STRESS; SOILS AB This study aimed to elucidate the relationship between plant hosts and root-colonizing fungi recovered from apple orchard soils that had been replanted over multiple generations. Functional relationships of three groups of filamentous fungi (Ceratobasidium sp., Cylindrocarpon-like group and Fusarium acuminatum) with apple rootstocks were evaluated in plant growth bioassays. The Cylindrocarpon-like group and Ceratobasidium sp. showed a relationship with the host plant varying from pathogenic to commensal through to mutualistic for the latter group, while that of F.acuminatum tended to be mutualistic. Seven fungal isolates of each group, which induced the highest plant growth in bioassays, were evaluated for auxin (IAA) and gibberellin (GA(3) and GA(4)) production in culture filtrate. All isolates of F.acuminatum as well as most of those of the Ceratobasidium sp. and Cylindrocarpon-like groups produced IAA in culture filtrate. IAA production was evaluated for additional isolates of endophytic fungal species from fruit tree orchards and the functionality of IAA was confirmed by growing in vitro micropropagated plantlets of apple rootstock on MS medium supplemented with fungal culture filtrate. Findings from this study may explain the difficulty in defining the precise role of diverse root-colonizing fungal populations in replant disease aetiology of fruit tree orchards. However, the results demonstrate the presence of a positive and widely available biotic component of the orchard soil biology that may be exploited for the benefit of tree growth and production. C1 [Manici, L. M.; Caputo, F.] Consiglio Ric & Sperimentaz Agr, CRA CIN, Bologna, Italy. [Kelderer, M.] Laimburg Res Ctr Agr & Forest, Ora, BZ, Italy. [Mazzola, M.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Manici, LM (reprint author), Consiglio Ric & Sperimentaz Agr, CRA CIN, Bologna, Italy. EM luisa.manici@entecra.it OI Manici, Luisa M./0000-0003-2798-4428 FU Italian Ministry of Politics for Agriculture, Food and Forestry through the project ENDOBIOFRUT; CORE Organic II Funding Bodies through the project BIO-INCROP [249667] FX The authors would like to thank A. R. Topp for her technical support in the plant growth bioassay and the staff of Renolab srl (San Giorgio di Piano, Bologna, Italy) for their support in analysis of fungal metabolites. The study was funded in part by the Italian Ministry of Politics for Agriculture, Food and Forestry through the project ENDOBIOFRUT and part by the CORE Organic II Funding Bodies, being partners of the FP7 ERA-Net project, CORE Organic II, www.coreorganic2.org (Coordination of European Transnational Research in Organic Food and Farming systems, project no. 249667), through the project BIO-INCROP. NR 35 TC 2 Z9 3 U1 7 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD AUG PY 2015 VL 64 IS 4 BP 843 EP 851 DI 10.1111/ppa.12315 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CM1XN UT WOS:000357474000012 ER PT J AU Gomes, LB Ward, TJ Badiale-Furlong, E Del Ponte, EM AF Gomes, L. B. Ward, T. J. Badiale-Furlong, E. Del Ponte, E. M. TI Species composition, toxigenic potential and pathogenicity of Fusarium graminearum species complex isolates from southern Brazilian rice SO PLANT PATHOLOGY LA English DT Article DE Fusarium asiaticum; nivalenol; Oryza sativa; trichothecene ID HEAD BLIGHT PATHOGENS; TRICHOTHECENE GENOTYPES; GENEALOGICAL CONCORDANCE; PARBOILED RICE; F-ASIATICUM; WHEAT; MYCOTOXINS; POPULATIONS; NIVALENOL; DEOXYNIVALENOL AB This study aimed to assess the extent and distribution of Fusarium graminearum species complex (FGSC) diversity in rice seeds produced in southern Brazil. Four species and two trichothecene genotypes were detected among 89 FGSC isolates, based on a multilocus genotyping assay: F.asiaticum (696%) with the nivalenol (NIV) genotype, F.graminearum (146%) with the 15-acetyldeoxynivalenol (ADON) genotype, and F.cortaderiae (146%) and F.meridionale (11%), both with the NIV genotype. Seven selected F.asiaticum isolates from rice produced NIV in rice-based substrate in vitro, at levels ranging from 47 to 841gg(-1). Similarly, two F.graminearum isolates from rice produced mainly 15-ADON (c.15-41gg(-1)) and a smaller amount of 3-ADON (c.6-12gg(-1)). One F.meridionale and two F.cortaderiae isolates did not produce detectable levels of trichothecenes. Two F.asiaticum isolates from rice and two from wheat (from a previous study), and one F.graminearum isolate from wheat, were pathogenic to both crops at various levels of aggressiveness based on measures of disease severity in wheat spikes and rice kernel infection in a greenhouse assay. Fusarium asiaticum and the reference F.graminearum isolate from wheat produced NIV, and deoxynivalenol and acetylates, respectively, in the kernels of inoculated wheat heads. No trichothecene was produced in kernels from inoculated rice panicles by any of the isolates. These findings constitute the first report of FGSC composition in rice outside Asia, and confirm the dominance of F.asiaticum in rice agroecosystems. C1 [Gomes, L. B.; Del Ponte, E. M.] Univ Fed Rio Grande do Sul, Dept Fitossanidade, BR-91540000 Porto Alegre, RS, Brazil. [Ward, T. J.] USDA ARS, Peoria, IL USA. [Badiale-Furlong, E.] Univ Fed Rio Grande, Escola Quim Alimentos, Rio Grande, RS, Brazil. RP Del Ponte, EM (reprint author), Univ Fed Vicosa, Dept Fitopatol, BR-36570900 Vicosa, MG, Brazil. EM delponte@ufv.br RI Del Ponte, Emerson/D-2464-2012 OI Del Ponte, Emerson/0000-0003-4398-409X FU Programa de Pos-graduacao em Fitotecnia of the Universidade Federal do Rio Grande do Sul (UFRGS); Conselho Nacional de desenvolvimento Cientifico e Tecnologico (CNPq); USDA-ARS National Program for Food Safety FX L.B.G. is grateful to the Programa de Pos-graduacao em Fitotecnia of the Universidade Federal do Rio Grande do Sul (UFRGS) and the Conselho Nacional de desenvolvimento Cientifico e Tecnologico (CNPq) for providing a graduate scholarship. E.M.D. and E.B.F. are grateful to the CNPq for a research fellowship. This study was partially supported by the USDA-ARS National Program for Food Safety. The authors are indebted to Nathane Orwig and Thomas Usgaard for excellent technical support. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 9 Z9 9 U1 2 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD AUG PY 2015 VL 64 IS 4 BP 980 EP 987 DI 10.1111/ppa.12332 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CM1XN UT WOS:000357474000026 ER PT J AU Habing, GG Kessler, SE Mollenkopf, DF Wittum, TE Anderson, TC Behravesh, CB Joseph, LA Erdman, MM AF Habing, G. G. Kessler, S. E. Mollenkopf, D. F. Wittum, T. E. Anderson, T. C. Behravesh, C. Barton Joseph, L. A. Erdman, M. M. TI Distribution and Diversity of Salmonella Strains in Shipments of Hatchling Poultry, United States, 2013 SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Salmonella; poultry; zoonoses; disease outbreaks; anti-microbial resistance; chickens ID PULSENET; HATCHERY; ANIMALS; FLOCKS AB Multistate outbreaks of salmonellosis associated with live poultry contact have been occurring with increasing frequency. In 2013, multistate outbreaks of salmonellosis were traced back to exposure to live poultry, some of which were purchased at a national chain of farm stores (Farm store chain Y). This study was conducted at 36 stores of Farm store chain Y and was concurrent with the timing of exposure for the human outbreaks of salmonellosis in 2013. We used environmental swabs of arriving shipment boxes of hatchling poultry and shipment tracking information to examine the distribution, diversity and anti-microbial resistance of non-typhoidal Salmonella (NTS) across farm stores and hatcheries. Isolates recovered from shipment boxes underwent serotyping, anti-microbial resistance (AMR) testing and pulsed-field gel electrophoresis (PFGE). Postal service tracking codes from the shipment boxes were used to determine the hatchery of origin. The PFGE patterns were compared with the PFGE patterns of NTS causing outbreaks of salmonellosis in 2013. A total of 219 hatchling boxes from 36 stores in 13 states were swabbed between 15 March 2013 and 18 April 2013. NTS were recovered from 59 (27%) of 219 hatchling boxes. Recovery was not significantly associated with species of hatchlings, number of birds in the shipment box, or the presence of dead, injured or sick birds. Four of the 23 PFGE patterns and 23 of 50 isolates were indistinguishable from strains causing human outbreaks in 2013. For serotypes associated with human illnesses, PFGE patterns most frequently recovered from shipment boxes were also more frequent causes of human illness. Boxes positive for the same PFGE pattern most frequently originated from the same mail-order hatchery. Only one of 59 isolates was resistant to anti-microbials used to treat Salmonella infections in people. This study provides critical information to address recurrent human outbreaks of salmonellosis associated with mail-order hatchling poultry. C1 [Habing, G. G.; Kessler, S. E.; Mollenkopf, D. F.; Wittum, T. E.] Ohio State Univ, Columbus, OH 43235 USA. [Anderson, T. C.; Behravesh, C. Barton; Joseph, L. A.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Erdman, M. M.] Natl Vet Serv Lab, Ames, IA 50010 USA. RP Habing, GG (reprint author), Ohio State Univ, A100D Sisson Hall,1920 Coffee Rd, Columbus, OH 43235 USA. EM habing.4@osu.edu FU Intramural CDC HHS [CC999999] NR 16 TC 0 Z9 0 U1 1 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD AUG PY 2015 VL 62 IS 5 BP 375 EP 380 DI 10.1111/zph.12157 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA CM3QS UT WOS:000357598900008 PM 25236179 ER PT J AU Eschen, R Britton, K Brockerhoff, E Burgess, T Dalley, V Epanchin-Niell, RS Gupta, K Hardy, G Huang, Y Kenis, M Kimani, E Li, HM Olsen, S Ormrod, R Otieno, W Sadof, C Tadeu, E Theyse, M AF Eschen, R. Britton, K. Brockerhoff, E. Burgess, T. Dalley, V. Epanchin-Niell, R. S. Gupta, K. Hardy, G. Huang, Y. Kenis, M. Kimani, E. Li, H. -M. Olsen, S. Ormrod, R. Otieno, W. Sadof, C. Tadeu, E. Theyse, M. TI International variation in phytosanitary legislation and regulations governing importation of plants for planting SO ENVIRONMENTAL SCIENCE & POLICY LA English DT Review DE International trade; Live plants; Invasive invertebrate pests and microbial pathogens; Legislation; IPPC; ISPM; Plants for planting; Risk analysis; Import regulations; Invasive alien species ID INVASIONS; INSECT; ESTABLISHMENT; INTERCEPTION; TRADE; RATES; PESTS; RISK; US AB The trade in plants for planting (P4P) is one of the major pathways for the introduction of pests. The strong increase in world trade in the past decades appears to have led to an increase in introductions of species transported by this pathway, and highlights the need for effective phytosanitary legislation and measures. The phytosanitary regulations in most countries are based on the International Plant Protection Convention and the World Trade Organisation's Agreement on Sanitary and Phytosanitary Measures, but there are large differences in countries' approaches to managing the risk of introducing invasive alien species through international plant trade. We reviewed elements of the phytosanitary legislations of ten countries on all continents and aimed to find regulations that prevent biological invasions. We found large differences in countries' phytosanitary regulations. New Zealand and Australia have the strictest phytosanitary regulations, while Europe maintains a general authorization for P4P imports. The remaining countries have regulations between these extremes. The evidence is sparse regarding the quality of implementation and effectiveness, and impact of individual phytosanitary measures. We recommend that National Plant Protection Organisations collect detailed information on P4P imports and the effectiveness of phytosanitary measures. Such information could provide a basis to improve a country's phytosanitary regulatory framework or could be used in risk assessments. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Eschen, R.; Kenis, M.] CABI, CH-2800 Delemont, Switzerland. [Britton, K.] USDA Forest Serv, Forest Serv Res & Dev, Washington, DC 20250 USA. [Brockerhoff, E.] Scion New Zealand Forest Res Inst, Christchurch 8540, New Zealand. [Burgess, T.; Hardy, G.] Murdoch Univ, Ctr Phytophthora Sci & Management, Sch Vet & Life Sci, Murdoch, WA 6150, Australia. [Dalley, V.; Olsen, S.] Minist Primary Ind, Plants Food & Environm Directorate, Wellington, New Zealand. [Epanchin-Niell, R. S.] Resources Future Inc, Washington, DC 20036 USA. [Gupta, K.] Natl Bur Plant Genet Resources, Div Plant Quarantine, New Delhi 110012, India. [Huang, Y.] CAIQ, PestRisk Anal Grp, Inst Plant Quarantine, Beijing, Peoples R China. [Kimani, E.] Kenyan Plant Hlth Inspectorate Serv, Nairobi, Kenya. [Li, H. -M.] Inst Plant Protect, MoA CABI Joint Lab Biosafety, Beijing 100193, Peoples R China. [Li, H. -M.] CABI, Zhongguancun Nandajie 12, Beijing 10080, Peoples R China. [Ormrod, R.] Canadian Food Inspect Agcy, Plant Hlth & Biosecur Programs, Kelowna, BC V1Y 7S9, Canada. [Otieno, W.] CABI, Nairobi, Kenya. [Sadof, C.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Tadeu, E.] Empresa Brasileira Pesquisa Agropecuaria Embrapa, BR-83411000 Colombo, PR, Brazil. [Theyse, M.] Fresh Produce Importers Assoc, ZA-0031 Gezina, South Africa. RP Eschen, R (reprint author), CABI, Rue Grillons 1, CH-2800 Delemont, Switzerland. EM r.eschen@cabi.org RI Burgess, Treena/G-4770-2011 OI Burgess, Treena/0000-0002-7962-219X FU National Socio-Environmental Synthesis Center (SESYNC) from the National Science Foundation [DBI-1052875]; Swiss Secretariat for Science, Education and Research FX We thank Bob Griffin, Brian Double and Bram de Hoop and an anonymous reviewer for comments on earlier versions of the manuscript. Part of this work was developed through working group 'Globalization of the Live Plant Trade: Informing Efficient Strategies for Reducing Non-Native Pest Invasion Risk' supported by the National Socio-Environmental Synthesis Center (SESYNC) under funding received from the National Science Foundation DBI-1052875. RE was supported through a grant from the Swiss Secretariat for Science, Education and Research to join the COST Action PERMIT. The inclusion of Kenya in the study greatly benefited from a CABI Development Bursary, awarded to RE. NR 34 TC 11 Z9 11 U1 5 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1462-9011 EI 1873-6416 J9 ENVIRON SCI POLICY JI Environ. Sci. Policy PD AUG PY 2015 VL 51 BP 228 EP 237 DI 10.1016/j.envsci.2015.04.021 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CL7JB UT WOS:000357147200020 ER PT J AU Simko, I Jimenez-Berni, JA Furbank, RT AF Simko, Ivan Jimenez-Berni, Jose A. Furbank, Robert T. TI Detection of decay in fresh-cut lettuce using hyperspectral imaging and chlorophyll fluorescence imaging SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Chlorophyll fluorescence imaging; Freezing damage; Fresh-cut lettuce salad; Hyperspectral imaging; Modified atmosphere packaging; Non-destructive phenotyping ID ESCHERICHIA-COLI O157H7; LEAFY VEGETABLES; QUALITY AB Fresh-cut lettuce sold in modified atmosphere packaging (MAP) is a desirable, but highly perishable product. Decay of tissue can start a few days after processing and may be difficult to detect by quick visual observation. A system for early detection of decay and gradual evaluation of its progress is important both for lettuce processing industry and for breeding companies and institutions assessing quality of new cultivars and breeding lines. We have developed two lettuce decay indices (LEDI) that can be used to detect decay of leaf tissue. One of the indices (LEDI4) is based on three wavelengths identified from hyperspectral imaging, while the second index (LEDICF) is based on chlorophyll fluorescence imaging. In addition to detecting lettuce decay, the indices identified tissue damaged by freezing temperatures. LEDI4 and LEDICF showed almost 97% accuracy in classifying tissue as being fresh or decayed when tested on red, dark green, green, light green, and yellow leaves. Specificity of the indices decreased when tested on fresh tissue with a very limited amount of chlorophyll that visually appeared to be almost white. Both indices detected lettuce decay without opening plastic MAP bags. The non-destructive nature of the methods thus allows rapid and repeated evaluation of samples over time and presents the opportunity for development of a commercial, high throughput scanner for evaluation of bagged, fresh-cut lettuce quality. Published by Elsevier B.V. C1 [Simko, Ivan] Agr Res Serv, USDA, US Agr Res Stn, Salinas, CA 93905 USA. [Jimenez-Berni, Jose A.; Furbank, Robert T.] CSIRO Plant Ind, Australian Plant Phen Facil, High Resolut Plant Phen Ctr, Canberra, ACT 2601, Australia. RP Simko, I (reprint author), Agr Res Serv, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM ivan.simko@ars.usda.gov RI Simko, Ivan/J-6048-2012; Furbank, Robert/A-1884-2010; Jimenez-Berni, Jose/F-8602-2011 OI Simko, Ivan/0000-0002-8769-8477; Jimenez-Berni, Jose/0000-0001-6542-921X FU OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems FX Ivan Simko acknowledges the receipt of a fellowship from the OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems in 2013. The authors wish to acknowledge the use of the Australian Plant Phenomics Facility in carrying out these experiments and the help of Helen Daily and the staff and students at HRPPC. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 25 TC 9 Z9 11 U1 2 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD AUG PY 2015 VL 106 BP 44 EP 52 DI 10.1016/j.postharvbio.2015.04.007 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CL8OC UT WOS:000357233000005 ER PT J AU Robins, JG Bushman, BS Escribano, S Jensen, KB AF Robins, Joseph G. Bushman, B. Shaun Escribano, Sandra Jensen, Kevin B. TI Heterosis for protein, digestibility, fiber, and water soluble carbohydrates in nine sources of orchardgrass germplasm SO EUPHYTICA LA English DT Article DE Carbohydrates; Cocksfoot; Dactylis glomerata; Digestibility; Fibre; Heterotic groups; Nutritive value; Protein ID DACTYLIS-GLOMERATA L; DRY-MATTER YIELD; GENETIC-VARIATION; PERFORMANCE; PROGRESS; MANAGEMENT; CROSSES; HYBRIDS; PROGENY; GRASS AB Orchardgrass, or cocksfoot, (Dactylis glomerata L.) is one of the most important forage grasses used in temperate agriculture. Yet, genetic gains are slow and complicated owing to the perennial and autotetraploid nature of orchardgrass. The development of orchardgrass population hybrids may be a way to more efficiently make genetic gains through heterosis. In the study reported herein, we describe the results of a 3 year study of the effect of heterosis on the forage quality of orchardgrass population hybrids managed with supplemental irrigation at a Cache County, UT, USA field site. The population hybrids derived from the orchardgrass cultivars Latar, Paiute, and Potomac; and from six additional germplasm sources from Asia, Europe, and North America. Data reflected July and August harvests from 2008 to 2010 and included crude protein (CP), in vitro true digestibility (IVTD), neutral detergent fiber (NDF), and water soluble carbohydrates (WSC). There were differences (P < 0.05) among the population hybrids and the included check cultivars for each of the traits at both harvest points. Mid-parent heterosis was relatively common and there were several instances of high-parent heterosis and reciprocal effects. Additionally, the population hybrids possessed better mean phenotypic values than the cultivars for July CP (2 g kg(-1)), and August CP (4 g kg(-1)), IVTD (7 g kg(-1)), NDF (-9 g kg(-1)), and WSC (4 g kg(-1)). Overall, the results suggest that population hybrids may prove a feasible approach for orchardgrass forage quality improvement. C1 [Robins, Joseph G.; Bushman, B. Shaun; Jensen, Kevin B.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Escribano, Sandra] Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA. RP Robins, JG (reprint author), USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. EM joseph.robins@ars.usda.gov NR 26 TC 3 Z9 3 U1 1 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD AUG PY 2015 VL 204 IS 3 BP 503 EP 511 DI 10.1007/s10681-014-1326-7 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CL3VU UT WOS:000356880300002 ER PT J AU Zhou, XL Zhang, Y Zeng, QD Chen, XM Han, DJ Huang, LL Kang, ZS AF Zhou, X. L. Zhang, Y. Zeng, Q. D. Chen, X. M. Han, D. J. Huang, L. L. Kang, Z. S. TI Identification of QTL for adult plant resistance to stripe rust in Chinese wheat landrace Caoxuan 5 SO EUPHYTICA LA English DT Article DE Durable resistance; Quantitative trait locus; Triticum aestivum; Puccinia striiformis f. sp tritici; Yellow rust ID TRITICUM-AESTIVUM L.; PUCCINIA-STRIIFORMIS; BREAD WHEAT; SPRING WHEAT; DISEASE RESISTANCE; LOCI; MAP; GENE; VALIDATION; LEAF AB Yellow (or stripe) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is an important wheat disease worldwide. The development of wheat cultivars with adult plant resistance (APR) has been given increasing emphasis in recent years because of the reputed durability of APR compared to all-stage resistance. Wheat landrace Caoxuan 5 showed a high level of APR in field nurseries from 2008 to 2014. Bulked segregant analysis using resistance gene analog polymorphism, simple sequence repeat and sequence-tagged site markers identified molecular markers linked to resistance genes. A total of 176 F-3 lines were evaluated for stripe rust response in field nurseries at two sites for 2 years. Broad sense heritability of APR in the population was estimated as 0.81. Inclusive composite interval mapping identified quantitative trait loci (QTL) QYrcx.nafu-3BS and QYrcx.nafu-6AL conferring adult plant resistance and they explained 17.3-38.1 % and 9.7-19.8 % of the phenotypic variations, respectively. Based on molecular markers, both QTL appear to be different from previously identified stripe rust resistance genes. The resistance QTL and their molecular markers identified in Caoxuan 5 should be useful in breeding for stripe rust resistance. C1 [Zhou, X. L.; Zeng, Q. D.; Huang, L. L.; Kang, Z. S.] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Zhou, X. L.; Zeng, Q. D.; Huang, L. L.; Kang, Z. S.] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China. [Zhang, Y.; Han, D. J.] Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China. [Chen, X. M.] Washington State Univ, USDA, ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Chen, X. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Kang, ZS (reprint author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. EM kangzs@nwsuaf.edu.cn FU National Key Basic Research Program of China [2013CB127700]; National Natural Science Foundation of China [31201497]; Chinese Ministry of Education [B07049] FX This study was supported by the National Key Basic Research Program of China (2013CB127700), the National Natural Science Foundation of China (31201497), and by the 111 Project from the Chinese Ministry of Education (No. B07049). We are grateful to the review of this manuscript by Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, Australia. NR 41 TC 0 Z9 0 U1 6 U2 31 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD AUG PY 2015 VL 204 IS 3 BP 627 EP 634 DI 10.1007/s10681-014-1349-0 PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CL3VU UT WOS:000356880300011 ER PT J AU Zhang, FF Liu, SS Chung, M Kelly, MJ AF Zhang, Fang Fang Liu, Shanshan Chung, Mei Kelly, Michael J. TI Growth patterns during and after treatment in patients with pediatric ALL: A meta-analysis SO PEDIATRIC BLOOD & CANCER LA English DT Article DE acute lymphoblastic leukemia; growth; obesity; pediatric; survivors ID ACUTE LYMPHOBLASTIC-LEUKEMIA; CHILDHOOD-CANCER SURVIVOR; BONE-MINERAL DENSITY; LONG-TERM SURVIVORS; BODY-MASS INDEX; CRANIAL IRRADIATION; ADULT SURVIVORS; CHILDREN; OBESITY; OVERWEIGHT AB BackgroundSurvivors of pediatric acute lymphoblastic leukemia (ALL) have a significantly higher body mass index (BMI) than their peers. Understanding the critical time periods in which patients with pediatric ALL are vulnerable to unhealthy weight gain will lay the groundwork for developing effectively timed interventions. ProcedureWe determined the growth patterns of patients with pediatric ALL during and after treatment through the conduct of a systematic review and meta-analysis. A search of MEDLINE, Scopus, and Web of Science was performed from its inception through May 2014. Studies met the inclusion criteria if they included at least 10 patients of pediatric ALL, and longitudinally assessed BMI at diagnosis and at least one time point after diagnosis ResultsTwenty-one studies met the inclusion criteria for the systematic review and 16 were included in meta-analysis. The mean increase in BMI z-score during treatment in 1,514 patients with pediatric ALL was 0.81 (95% CI: 0.25-1.38). Specifically, patients experienced substantial weight gain in early treatment (=0.41, 95% CI: -0.34, 1.17) and again during maintenance (=0.34, 95% CI:-0.22, 0.90). The mean increase in BMI z-score ranged between 0.52 and 0.89 beyond treatment completion. Subgroup analyses found unhealthy weight gain occurred regardless of patients' receipt of cranial radiation therapy, sex, and, weight status at diagnosis. ConclusionsPatients with pediatric ALL experience unhealthy weight gain early in treatment, and increases in weight are maintained beyond treatment completion. Preventing early onset of obesity is a priority for improving the care and outcomes for patients with pediatric ALL. Pediatr Blood Cancer 2015;62:1452-1460. (c) 2015 Wiley Periodicals, Inc. C1 [Zhang, Fang Fang; Liu, Shanshan] Tufts Univ, Dept Nutr Sci, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Zhang, Fang Fang] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chung, Mei] Tufts Univ, Nutr Infect Unit, Dept Publ Hlth & Community Med, Sch Med, Boston, MA 02111 USA. [Kelly, Michael J.] Tufts Med Ctr, Floating Hosp Children, Div Pediat Hematol Oncol, Boston, MA USA. [Kelly, Michael J.] Tufts Univ, Sch Med, Dept Pediat, Boston, MA 02111 USA. RP Zhang, FF (reprint author), Tufts Univ, Dept Nutr Sci, Friedman Sch Nutr Sci & Policy 28, 150 Harrison Ave,29, Boston, MA 02111 USA. EM fang_fang.zhang@tufts.edu FU Boston Nutrition Obesity Research Centerr [P30DK46200]; National Center for Research Resources [UL1 RR025752]; National Center for Advancing Translational Sciences; National Institutes of Health [UL1TR000073] FX Grant sponsor: Boston Nutrition Obesity Research Centerr; Grant number: P30DK46200; Grant sponsor: National Center for Research Resources; Grant number: UL1 RR025752; Grant sponsor: National Center for Advancing Translational Sciences and the National Institutes of Health; Grant number: UL1TR000073 NR 47 TC 8 Z9 8 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1545-5009 EI 1545-5017 J9 PEDIATR BLOOD CANCER JI Pediatr. Blood Cancer PD AUG PY 2015 VL 62 IS 8 BP 1452 EP 1460 DI 10.1002/pbc.25519 PG 9 WC Oncology; Hematology; Pediatrics SC Oncology; Hematology; Pediatrics GA CL3RD UT WOS:000356867200022 PM 25808413 ER PT J AU Zhang, FF Saltzman, E Kelly, MJ Liu, SS Must, A Parsons, SK Roberts, SB AF Zhang, Fang Fang Saltzman, Edward Kelly, Michael J. Liu, Shanshan Must, Aviva Parsons, Susan K. Roberts, Susan B. TI Comparison of childhood cancer survivors' nutritional intake with US dietary guidelines SO PEDIATRIC BLOOD & CANCER LA English DT Article DE childhood cancer; nutrition; survivors ID ACUTE LYMPHOBLASTIC-LEUKEMIA; LONG-TERM SURVIVORS; BODY-MASS INDEX; ADULT SURVIVORS; RISK-FACTORS; CARDIOVASCULAR-DISEASE; METABOLIC SYNDROME; 5-YEAR SURVIVORS; LATE MORTALITY; MULTIPLE-PASS AB BackgroundDespite improved survival, childhood cancer survivors experience significantly elevated risk of premature mortality and serious morbidity due to chronic health conditions. Poor diet quality can exacerbate chronic health conditions in the survivors but their nutritional intake has not been adequately studied. ProcedureWe assessed the Healthy Eating Index 2010 (HEI-2010) in 22 survivors of pediatric acute lymphoblastic leukemia and lymphoma (median age=11.7 years) and compared survivors' dietary intake to the 2010 Dietary Guidelines for Americans. Dietary data were collected using repeated 24hr dietary recalls over a 1-year period, which were averaged to estimate habitual intake. ResultsThe mean HEI-2010 in childhood cancer survivors was 52.7, about 50 percent of the maximum score. Long-term survivors (time from diagnosis 10 years) had a significantly lower HEI-2010 than recent survivors (time from diagnosis <5 years) (=-11.5, 95% CI: -22.1, -0.9, P=0.047). For individual food groups and nutrients, survivors had a particularly poor adherence to green vegetables and beans, total vegetables, and whole fruits. None of the survivors met the guidelines for dietary fiber and potassium intake. Only 4%, 19%, 24%, and 29% met the guidelines for vitamin D, sodium, calcium, and saturated fat intake. The average intake in relative to the recommended intake was 32% for vitamin D, 50% for potassium, 63% for fiber, and 85% for calcium, but was 115% for saturated fat and 143% for sodium. ConclusionsChildhood cancer survivors, in particular long-term survivors, have a poor adherence to the US dietary guidelines. Pediatr Blood Cancer 2015;62:1461-1467. (c) 2015 Wiley Periodicals, Inc. C1 [Zhang, Fang Fang; Saltzman, Edward; Liu, Shanshan; Roberts, Susan B.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Dept Nutr Sci, Boston, MA 02111 USA. [Zhang, Fang Fang; Saltzman, Edward; Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Must, Aviva] Tufts Univ, Dept Publ Hlth & Community Med, Sch Med, Boston, MA 02111 USA. [Kelly, Michael J.; Parsons, Susan K.] Tufts Med Ctr, Floating Hosp Children, Div Pediat Hematol Oncol, Boston, MA USA. [Kelly, Michael J.] Tufts Univ, Dept Pediat, Sch Med, Boston, MA 02111 USA. [Parsons, Susan K.] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Boston, MA USA. [Parsons, Susan K.] Tufts Univ, Dept Med, Sch Med, Boston, MA 02111 USA. RP Zhang, FF (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Dept Nutr Sci, Boston, MA 02111 USA. EM fang_fang.zhang@tufts.edu FU National Center for Research Resources [UL1 RR025752]; National Institutes of Health [UL1 TR000073]; Boston Nutrition Obesity Research Center [P30DK46200] FX Grant sponsor: National Center for Research Resources; Grant number: UL1 RR025752; Grant sponsor: National Institutes of Health; Grant number: UL1 TR000073; Grant sponsor: Boston Nutrition Obesity Research Center; Grant number: P30DK46200 NR 49 TC 5 Z9 5 U1 1 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1545-5009 EI 1545-5017 J9 PEDIATR BLOOD CANCER JI Pediatr. Blood Cancer PD AUG PY 2015 VL 62 IS 8 BP 1461 EP 1467 DI 10.1002/pbc.25521 PG 7 WC Oncology; Hematology; Pediatrics SC Oncology; Hematology; Pediatrics GA CL3RD UT WOS:000356867200023 PM 25808589 ER PT J AU Anderson, RL AF Anderson, Randy L. TI A cultural system to reduce weed interference in organic soybean SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE cover crops; cultural tactics; rotation design; weed biomass ID NO-TILL; CONSERVATION AGRICULTURE; POPULATION-DYNAMICS; CONYZA-CANADENSIS; CROP ROTATIONS; COVER CROPS; EMERGENCE; YIELD; CORN; TERMINATION AB Organic producers are seeking alternative tactics for weed control, so that they can reduce their need for tillage. In this study, we examined cultural strategies for controlling weeds during the transition from a cool-season crop to soybean. The study was arranged as a two-way factorial, with factors being choice of cool-season crop and tillage treatments. The cool-season crops were either spring wheat harvested for grain or an oat-pea mixture harvested for forage. Five tillage treatments, ranging from intensive tillage to no-till, were established following each cool-season crop. Two tillage treatments included the cover crops, oat plus oilseed radish. Soybean was planted the following growing season. Each soybean plot was split into two subplots: weed-free and weed-infested. A cultural system comprising oat/pea as a preceding crop with no-till and cover crops reduced weed biomass in soybean 63% compared to intensive tillage. Reduced weed biomass resulted because of delayed weed emergence and lower weed community density. Consequently, soybean yielded 14% more in this treatment than with the intensive tillage treatment when weeds were present. Weed community composition also differed between the two systems; horseweed and field dandelion were prominent in no-till, whereas common lambsquarters, redroot pigweed and buffalobur were prevalent in the tillage control. Other treatments did not control weeds better than intensive tillage. A cultural system approach may minimize the need for tillage during the interval between cool-season crops and soybean. C1 USDA ARS, Brookings, SD 57006 USA. RP Anderson, RL (reprint author), USDA ARS, Brookings, SD 57006 USA. EM randy.anderson@ars.usda.gov NR 36 TC 1 Z9 1 U1 3 U2 29 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD AUG PY 2015 VL 30 IS 4 BP 392 EP 398 DI 10.1017/S1742170514000167 PG 7 WC Agriculture, Multidisciplinary SC Agriculture GA CL2YG UT WOS:000356813400007 ER PT J AU Lee, JS Yoo, MH Jung, JK Bilyeu, KD Lee, JD Kang, S AF Lee, Ju Seok Yoo, Min-ho Jung, Jin Kyo Bilyeu, Kristin D. Lee, Jeong-Dong Kang, Sungtaeg TI Detection of novel QTLs for foxglove aphid resistance in soybean SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID AULACORTHUM-SOLANI KALTENBACH; HEMIPTERA APHIDIDAE; GENE; STRAINS; VIRUS; LOCI; MAP AB The Raso2 , novel QTL for Korea biotype foxglove aphid resistance in soybean from PI 366121 was identified on chromosome 7 using GoldenGate SNP microarray. Foxglove aphid, Aulacorthum solani (Kaltenbach), is a hemipteran insect that infects a wide variety of plants worldwide and causes serious yield losses in crops. The objective of this study was to identify the putative QTL for foxglove aphid resistance in wild soybean, PI 366121, (Glycine soja Sieb. and Zucc.). One hundred and forty-one F-4-derived F-8 recombinant inbred lines developed from a cross of susceptible Williams 82 and PI 366121 were used. The phenotyping of antibiosis and antixenosis resistance was done through choice and no-choice tests with total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 504 single-nucleotide polymorphism markers utilizing a GoldenGate assay. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 and three minor QTL regions on chromosomes 3, 6 and 18 were identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. However, the minor QTLs showed only antixenosis resistance response. The major QTL mapped to a different chromosome than the previously identified foxglove aphid resistance QTL, Raso1, from the cultivar Adams. Also, the responses to the Korea biotype foxglove aphid were different for Raso1, and the gene from PI 366121 against the Korea biotype foxglove aphid was different. Thus, the foxglove aphid resistance gene from PI 366121 was determined to be an independent gene from Raso1 and was designated as Raso2. This result could be useful in breeding for new foxglove aphid-resistant soybean cultivars. C1 [Lee, Ju Seok; Yoo, Min-ho; Kang, Sungtaeg] Dankook Univ, Dept Crop Sci & Biotechnol, Cheonan 330714, South Korea. [Jung, Jin Kyo] RDA, Natl Inst Crop Sci, Suwon 441857, South Korea. [Bilyeu, Kristin D.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. [Lee, Jeong-Dong] Kyungpook Natl Univ, Div Plant Biosci, Daegu 702701, South Korea. RP Kang, S (reprint author), Dankook Univ, Dept Crop Sci & Biotechnol, Cheonan 330714, South Korea. EM kangst@dankook.ac.kr FU 'Cooperative Research Program for Agriculture Science & Technology Development' Rural Development Administration, Republic of Korea [PJ009771] FX This work was carried out with the support of 'Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009771)' Rural Development Administration, Republic of Korea. NR 37 TC 3 Z9 3 U1 0 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2015 VL 128 IS 8 BP 1481 EP 1488 DI 10.1007/s00122-015-2519-8 PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CL2MN UT WOS:000356778300003 PM 25904004 ER PT J AU Cichy, KA Wiesinger, JA Mendoza, FA AF Cichy, Karen A. Wiesinger, Jason A. Mendoza, Fernando A. TI Genetic diversity and genome-wide association analysis of cooking time in dry bean (Phaseolus vulgaris L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID HARD-TO-COOK; COMMON BEANS; NUTRITIONAL QUALITY; ARABIDOPSIS; SOFTWARE; TANZANIA; SAMPLES; TRAITS; ENERGY; SEEDS AB Fivefold diversity for cooking time found in a panel of 206 Phaseolus vulgaris accessions. Fastest accession cooks nearly 20 min faster than average. SNPs associated with cooking time on Pv02, 03, and 06. Dry beans (Phaseolus vulgaris L.) are a nutrient dense food and a dietary staple in parts of Africa and Latin America. One of the major factors that limits greater utilization of beans is their long cooking times compared to other foods. Cooking time is an important trait with implications for gender equity, nutritional value of diets, and energy utilization. Very little is known about the genetic diversity and genomic regions involved in determining cooking time. The objective of this research was to assess cooking time on a panel of 206 P. vulgaris accessions, use genome- wide association analysis (GWAS) to identify genomic regions influencing this trait, and to test the ability to predict cooking time by raw seed characteristics. In this study 5.5-fold variation for cooking time was found and five bean accessions were identified which cook in less than 27 min across 2 years, where the average cooking time was 37 min. One accession, ADP0367 cooked nearly 20 min faster than average. Four of these five accessions showed close phylogenetic relationship based on a NJ tree developed with similar to 5000 SNP markers, suggesting a potentially similar underlying genetic mechanism. GWAS revealed regions on chromosomes Pv02, Pv03, and Pv06 associated with cooking time. Vis/NIR scanning of raw seed explained 68 % of the phenotypic variation for cooking time, suggesting with additional experimentation, it may be possible to use this spectroscopy method to non-destructively identify fast cooking lines as part of a breeding program. C1 [Cichy, Karen A.] ARS, Sugarbeet & Bean Res Unit, USDA, E Lansing, MI 48824 USA. [Cichy, Karen A.; Wiesinger, Jason A.; Mendoza, Fernando A.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Mendoza, Fernando A.] Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA. RP Cichy, KA (reprint author), ARS, Sugarbeet & Bean Res Unit, USDA, 1066 Bogue St,Room 494, E Lansing, MI 48824 USA. EM karen.cichy@ars.usda.gov OI Cichy, Karen/0000-0002-4311-0774 FU Norman Borlaug Commemorative Research Initiative (US Agency for International Development); US Department of Agriculture, Agricultural Research Service FX This work was supported in part by funding from the Norman Borlaug Commemorative Research Initiative (US Agency for International Development), by the US Department of Agriculture, Agricultural Research Service. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. We also thank Yasmin Salat, Dennis Katuuramu, and Scott Shaw for assistance with cooking. NR 49 TC 5 Z9 5 U1 5 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2015 VL 128 IS 8 BP 1555 EP 1567 DI 10.1007/s00122-015-2531-z PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CL2MN UT WOS:000356778300009 PM 26003191 ER PT J AU Hystad, SM Martin, JM Graybosch, RA Giroux, MJ AF Hystad, S. M. Martin, J. M. Graybosch, R. A. Giroux, M. J. TI Genetic characterization and expression analysis of wheat (Triticum aestivum) line 07OR1074 exhibiting very low polyphenol oxidase (PPO) activity SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID COMMON WHEAT; DURUM-WHEAT; ALLELIC VARIATION; NOODLE COLOR; HARD WHITE; MARKERS; REGISTRATION; PUROINDOLINE; INHERITANCE; PRODUCTS AB Characterized novel mutations present at Ppo loci account for the substantial reduction of the total kernel PPO activity present in a putative null Ppo - A1 genetic background. Wheat (Triticum aestivum) polyphenol oxidase (PPO) contributes to the time-dependent discoloration of Asian noodles. Wheat contains multiple paralogous and orthologous Ppo genes, Ppo-A1, Ppo-D1, Ppo-A2, Ppo-D2, and Ppo-B2, expressed in wheat kernels. To date, wheat noodle color improvement efforts have focused on breeding cultivars containing Ppo-D1 and Ppo-A1 alleles conferring reduced PPO activity. A major impediment to wheat quality improvement is a lack of additional Ppo alleles conferring reduced kernel PPO. In this study, a previously reported very low PPO line, 07OR1074, was found to contain a novel allele at Ppo-A2 and null alleles at the Ppo-A1 and Ppo-D1 loci. To examine the impact of each mutation upon kernel PPO, populations were generated from crosses between 07OR1074 and the hard white spring wheat cultivars Choteau and Vida. Expression analysis using RNA-seq demonstrated no detectable Ppo-A1 transcripts in 07OR1074 while Ppo-D1 transcripts were present at less than 10 % of that seen in Choteau and Vida. Novel markers specific for the Ppo-D1 and Ppo-A2 mutations discovered in 07OR1074, along with the Ppo-A1 STS marker, were used to screen segregating populations. Evaluation of lines indicated a substantial genotypic effect on PPO with Ppo-A1 and Ppo-D1 alleles contributing significantly to total PPO in both populations. These results show that the novel mutations in Ppo-A1 and Ppo-D1 present in 07OR1074 are both important to lowering overall wheat seed PPO activity and may be useful to produce more desirable and marketable wheat-based products. C1 [Hystad, S. M.; Martin, J. M.; Giroux, M. J.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Graybosch, R. A.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RP Giroux, MJ (reprint author), Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. EM mgiroux@montana.edu FU Montana Wheat and Barley Committee FX We thank the Montana Wheat and Barley Committee for financial support. NR 39 TC 1 Z9 1 U1 5 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2015 VL 128 IS 8 BP 1605 EP 1615 DI 10.1007/s00122-015-2535-8 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CL2MN UT WOS:000356778300013 PM 25982131 ER PT J AU Liang, Y Zhang, DY Ouyang, SH Xie, JZ Wu, QH Wang, ZZ Cui, Y Lu, P Zhang, D Liu, ZJ Zhu, J Chen, YX Zhang, Y Luo, MC Dvorak, J Huo, NX Sun, QX Gu, YQ Liu, ZY AF Liang, Yong Zhang, De-Yun Ouyang, Shuhong Xie, Jingzhong Wu, Qiuhong Wang, Zhenzhong Cui, Yu Lu, Ping Zhang, Dong Liu, Zi-Ji Zhu, Jie Chen, Yong-Xing Zhang, Yan Luo, Ming-Cheng Dvorak, Jan Huo, Naxin Sun, Qixin Gu, Yong-Qiang Liu, Zhiyong TI Dynamic evolution of resistance gene analogs in the orthologous genomic regions of powdery mildew resistance gene MlIW170 in Triticum dicoccoides and Aegilops tauschii SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID HEXAPLOID WHEAT; DRAFT GENOME; COMMON WHEAT; ORYZA-SATIVA; AESTIVUM L.; WILD EMMER; LEAF RUST; A-GENOME; RECOMBINATION; PROGENITOR AB Rapid evolution of powdery mildew resistance gene MlIW170 orthologous genomic regions in wheat subgenomes. Wheat is one of the most important staple grain crops in the world and also an excellent model for plant ploidy evolution research with different ploidy levels from diploid to hexaploid. Powdery mildew disease caused by Blumeria graminis f.sp. tritici can result in significant loss in both grain yield and quality in wheat. In this study, the wheat powdery mildew resistance gene MlIW170 locus located at the Triticum dicoccoides chromosome 2B short arm was further characterized by constructing and sequencing a BAC-based physical map contig covering a 0.3 cM genetic distance region (880 kb) and developing additional markers to delineate the resistance gene within a 0.16 cM genetic interval (372 kb). Comparative analyses of the T. dicoccoides 2BS region with the orthologous Aegilops tauschii 2DS region showed great gene colinearity, including the structure organization of both types of RGA1/2-like and RPS2-like resistance genes. Comparative analyses with the orthologous regions from Brachypodium and rice genomes revealed considerable dynamic evolutionary changes that have re-shaped this MlIW170 region in the wheat genome, resulting in a high number of non-syntenic genes including resistance-related genes. This result might reflect the rapid evolution in R-gene regions. Phylogenetic analysis on these resistance-related gene sequences indicated the duplication of these genes in the MlIW170 region, occurred before the separation of the wheat B and D genomes. C1 [Liang, Yong; Zhang, De-Yun; Ouyang, Shuhong; Xie, Jingzhong; Wu, Qiuhong; Wang, Zhenzhong; Cui, Yu; Lu, Ping; Zhang, Dong; Liu, Zi-Ji; Zhu, Jie; Chen, Yong-Xing; Zhang, Yan; Sun, Qixin; Liu, Zhiyong] China Agr Univ, Dept Plant Genet & Breeding, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China. [Luo, Ming-Cheng; Dvorak, Jan] Univ Calif Davis, Davis, CA 95616 USA. [Huo, Naxin; Gu, Yong-Qiang] ARS, USDA, West Reg Res Ctr, Albany, CA 94710 USA. RP Gu, YQ (reprint author), China Agr Univ, Dept Plant Genet & Breeding, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China. EM yong.gu@ars.usda.gov; zhiyongliu@cau.edu.cn FU Ministry of Science and Technology of China [2011AA100104, 2013CB127705]; Ministry of Agriculture of China [2011ZX08009-003-001]; National Natural Science Foundation of China [31030056]; Program of Introducing Talents of Discipline to Universities [111-02-3]; Monsanto's Beachell-Borlaug International Scholars Program FX This work was financially supported by Ministry of Science and Technology of China (2011AA100104, 2013CB127705), Ministry of Agriculture of China (2011ZX08009-003-001), National Natural Science Foundation of China (31030056), the Program of Introducing Talents of Discipline to Universities (111-02-3), and Monsanto's Beachell-Borlaug International Scholars Program. NR 38 TC 0 Z9 0 U1 1 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2015 VL 128 IS 8 BP 1617 EP 1629 DI 10.1007/s00122-015-2536-7 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CL2MN UT WOS:000356778300014 PM 25993896 ER PT J AU Bushakra, JM Bryant, DW Dossett, M Vining, KJ VanBuren, R Gilmore, BS Lee, J Mockler, TC Finn, CE Bassil, NV AF Bushakra, Jill M. Bryant, Douglas W. Dossett, Michael Vining, Kelly J. VanBuren, Robert Gilmore, Barbara S. Lee, Jungmin Mockler, Todd C. Finn, Chad E. Bassil, Nahla V. TI A genetic linkage map of black raspberry (Rubus occidentalis) and the mapping of Ag (4) conferring resistance to the aphid Amphorophora agathonica SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID REPEAT SSR MARKERS; DOMESTICA BORKH. GENOME; PERSICA L. BATSCH; RED RASPBERRY; MICROSATELLITE MARKERS; RAPD MARKERS; CANDIDATE GENES; PEACH; IDENTIFICATION; APPLE AB We have constructed a densely populated, saturated genetic linkage map of black raspberry and successfully placed a locus for aphid resistance. Black raspberry (Rubus occidentalis L.) is a high-value crop in the Pacific Northwest of North America with an international marketplace. Few genetic resources are readily available and little improvement has been achieved through breeding efforts to address production challenges involved in growing this crop. Contributing to its lack of improvement is low genetic diversity in elite cultivars and an untapped reservoir of genetic diversity from wild germplasm. In the Pacific Northwest, where most production is centered, the current standard commercial cultivar is highly susceptible to the aphid Amphorophora agathonica Hottes, which is a vector for the Raspberry mosaic virus complex. Infection with the virus complex leads to a rapid decline in plant health resulting in field replacement after only 3-4 growing seasons. Sources of aphid resistance have been identified in wild germplasm and are used to develop mapping populations to study the inheritance of these valuable traits. We have constructed a genetic linkage map using single-nucleotide polymorphism and transferable (primarily simple sequence repeat) markers for F-1 population ORUS 4305 consisting of 115 progeny that segregate for aphid resistance. Our linkage map of seven linkage groups representing the seven haploid chromosomes of black raspberry consists of 274 markers on the maternal map and 292 markers on the paternal map including a morphological locus for aphid resistance. This is the first linkage map of black raspberry and will aid in developing markers for marker-assisted breeding, comparative mapping with other Rubus species, and enhancing the black raspberry genome assembly. C1 [Bushakra, Jill M.; Gilmore, Barbara S.; Bassil, Nahla V.] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Bryant, Douglas W.; VanBuren, Robert; Mockler, Todd C.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Dossett, Michael] Agr & Agri Food Canada, BC Blueberry Council, Pacific Agrifood Res Ctr, Agassiz, BC V0M 1A0, Canada. [Vining, Kelly J.; Mockler, Todd C.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Gilmore, Barbara S.] ARS, USDA, Natl Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Lee, Jungmin] ARS, USDA, Hort Crops Res Unit Worksite, Parma, ID 83660 USA. [Finn, Chad E.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Bushakra, JM (reprint author), ARS, USDA, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM jill.bushakra@ars.usda.gov RI Mockler, Todd/L-2609-2013; OI Mockler, Todd/0000-0002-0462-5775; Lee, Jungmin/0000-0002-8660-9444 FU Washington Red Raspberry Commission; Oregon Raspberry and Blackberry Commission; North American Raspberry and Blackberry Growers Association; USDA-ARS; Northwest Center for Small Fruit Research; USDA-National Institute of Food and Agriculture (NIFA) Specialty Crop Research Initiative (SCRI) USDA-ARS CRIS [2072-21000-044-00D, 2072-21000-047-00D, 2072-21220-002-00D] FX The authors thank Dr. Jana Lee (USDA-ARS HCRU, Corvallis, OR, USA) for her help with rearing aphids, Dr. Eric van de Weg (University of Wageningen, The Netherlands) for his assistance with graphical genotyping and consultation, The Barley Lab at Oregon State University for access to JoinMap, Drs. Kim Lewers (USDA-ARS BARC, Beltsville, MD, USA) and Christopher Saski (Clemson University, Clemson, SC, USA) for access to unpublished SSR marker data, and Drs. Kim Lewers, James Olmstead (University of Florida, Gainesville, FL, USA) and two anonymous reviewers for their helpful suggestions. The authors thank the Washington Red Raspberry Commission, the Oregon Raspberry and Blackberry Commission, the North American Raspberry and Blackberry Growers Association, the USDA-ARS, and the Northwest Center for Small Fruit Research for providing matching funds or other support. This work was funded by the USDA-National Institute of Food and Agriculture (NIFA) Specialty Crop Research Initiative (SCRI) USDA-ARS CRIS 2072-21000-044-00D, 2072-21000-047-00D and 2072-21220-002-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 97 TC 6 Z9 6 U1 2 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2015 VL 128 IS 8 BP 1631 EP 1646 DI 10.1007/s00122-015-2541-x PG 16 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CL2MN UT WOS:000356778300015 PM 26037086 ER PT J AU Bytnerowicz, A Johnson, RF Zhang, L Jenerette, GD Fenn, ME Schilling, SL Gonzalez-Fernandez, I AF Bytnerowicz, A. Johnson, R. F. Zhang, L. Jenerette, G. D. Fenn, M. E. Schilling, S. L. Gonzalez-Fernandez, I. TI An empirical inferential method of estimating nitrogen deposition to Mediterranean-type ecosystems: the San Bernardino Mountains case study SO ENVIRONMENTAL POLLUTION LA English DT Article DE Nitrogen deposition; Complex terrain; Forests; Inferential deposition model; Critical loads ID BIDIRECTIONAL AMMONIA EXCHANGE; GASEOUS DRY DEPOSITION; MIXED-CONIFER FOREST; AMBIENT NITRIC-ACID; SOUTHERN CALIFORNIA; AIR-POLLUTANTS; ATMOSPHERIC DEPOSITION; UNITED-STATES; NORTH-AMERICA; ECOLOGICAL PERSPECTIVE AB The empirical inferential method (ELM) allows for spatially and temporally-dense estimates of atmospheric nitrogen (N) deposition to Mediterranean ecosystems. This method, set within a GIS platform, is based on ambient concentrations of NH3, NO, NO2 and HNO3; surface conductance of NH4+ and NO3-; stomatal conductance of NH3, NO, NO2 and HNO3; and satellite-derived LAI. Estimated deposition is based on data collected during 2002-2006 in the San Bernardino Mountains (SBM) of southern California. Approximately 2/3 of dry N deposition was to plant surfaces and 1/3 as stomatal uptake. Summer-season N deposition ranged from <3 kg ha(-1) in the eastern SBM to similar to 60 kg ha(-1) in the western SBM near the Los Angeles Basin and compared well with the throughfall and big-leaf micrometeorological inferential methods. Extrapolating summertime N deposition estimates to annual values showed large areas of the SBM exceeding critical loads for nutrient N in chaparral and mixed conifer forests. Published by Elsevier Ltd. C1 [Bytnerowicz, A.; Fenn, M. E.; Schilling, S. L.] US Forest Serv, USDA, PSW Res Stn, Riverside, CA 92507 USA. [Johnson, R. F.; Jenerette, G. D.] Univ Calif Riverside, Ctr Conservat Biol & Bot & Plant Sci, Riverside, CA 92521 USA. [Zhang, L.] Environm Canada, Air Qual Res Div, Toronto, ON MH3 5T4, Canada. [Gonzalez-Fernandez, I.] CIEMAT, Ecotoxicol Contaminac Atmosfer, E-28040 Madrid, Spain. RP Bytnerowicz, A (reprint author), US Forest Serv, USDA, PSW Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM abytnerowicz@fs.fed.us; mfenn@fs.fed.us RI Gonzalez Fernandez, Ignacio/Q-8214-2016 FU National Science Foundation [DEB 04-21530, DEB 0919006]; Fulbright-Schuman grant; EDEN project [CGL2009-13188-C03-02]; FP7 ECLAIRE project [EU-FP7-ENV-2011] FX Landcover and LAI data are available from the United States Forest Service and National Aeronautics and Space Administration (https://lpdaac.usgs.gov/). Gas concentration data are available on request by contacting the corresponding author. Study was partially funded by the National Science Foundation Biocomplexity Project DEB 04-21530 and DEB 0919006. Ignacio Gonzalez-Fernandez participation was supported by a Fulbright-Schuman grant and the EDEN (CGL2009-13188-C03-02) and FP7 ECLAIRE (EU-FP7-ENV-2011) projects. The authors thank Haiganoush Preisler for discussion of methodologies used in the project, especially analysis of uncertainty. NR 86 TC 4 Z9 4 U1 8 U2 38 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD AUG PY 2015 VL 203 BP 69 EP 88 DI 10.1016/j.envpol.2015.03.028 PG 20 WC Environmental Sciences SC Environmental Sciences & Ecology GA CL0OW UT WOS:000356643000009 PM 25863884 ER PT J AU Quan, B Bai, YJ Romkens, MJM Chang, KT Song, H Guo, T Lei, S AF Quan, Bin Bai, Yijun Roemkens, M. J. M. Chang, Kang-tsung Song, Hui Guo, Tao Lei, Shi TI Urban land expansion in Quanzhou City, China, 1995-2010 SO HABITAT INTERNATIONAL LA English DT Article DE Quanzhou; Urban land expansion; Land use change; Population growth; In situ urbanization ID QUANTIFYING SPATIOTEMPORAL PATTERNS; SPATIAL-TEMPORAL PATTERN; MULTISCALE PERSPECTIVE; RURAL CHINA; TIME-SERIES; CITIES; URBANIZATION; GROWTH; COMPACT; METRICS AB With its phenomenal development in recent decades, urbanization in China has been covered in a large number of studies. These studies have focused on large cities, with smaller and lesser known cities largely overlooked. This study analyzed the spatiotemporal changes of land use in Quanzhou, a historical city in Fujian Province, using GIS and remote sensing tools. Based on the land use change indices and spatial metrics, our results showed that built-up (urban) land in Quanzhou increased more than twofold in 1995-2010, at the expense of cultivated land, woodland, and grassland. During the same period, urban land patches increased in both number and size, while becoming more irregular and complex in shape. Most urban land expansion took place in the coastal areas, including the city districts and development and industrial zones. Although urbanization in Quanzhou has been remarkable since 1995, its average rate of urban land expansion has fallen behind Shenzhen and Dongguan in the Pearl River Delta. Geographic location and population growth are two important factors for the difference. Quanzhou is located in a less developed region of China, and its population growth has been slow due to its heavy reliance on labor-intensive, low-technology industries, which do not offer sufficient rural-urban wage differential to attract large inflows of migrant workers. Urbanization in China follows different paths in different cities and regions, as shown in this study by comparing Quanzhou with cities in the Pearl River Delta. (c) 2015 Elsevier Ltd. All rights reserved. C1 [Quan, Bin; Bai, Yijun; Song, Hui; Guo, Tao; Lei, Shi] Hunan Univ Sci & Technol, Natl Local Joint Engn Lab Geospatial Informat Tec, Xiangtan 411201, Peoples R China. [Roemkens, M. J. M.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Chang, Kang-tsung] Kainan Univ, Luzhu 33857, Taoyuan County, Taiwan. RP Chang, KT (reprint author), Kainan Univ, 1 Kainan Rd, Luzhu 33857, Taoyuan County, Taiwan. EM quanbin308@aliyun.com; chang@uidaho.edu FU Hunan Provincial Natural Science Foundation of China [14JJ2098] FX This research was supported by the Hunan Provincial Natural Science Foundation of China (No. 14JJ2098). NR 48 TC 5 Z9 5 U1 8 U2 42 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0197-3975 EI 1873-5428 J9 HABITAT INT JI Habitat Int. PD AUG PY 2015 VL 48 BP 131 EP 139 DI 10.1016/j.habitatint.2015.03.021 PG 9 WC Environmental Studies; Planning & Development; Urban Studies SC Environmental Sciences & Ecology; Public Administration; Urban Studies GA CK3JU UT WOS:000356113200015 ER PT J AU Centi, AJ Brown-Ramos, M Haytowitz, DB Booth, SL AF Centi, Amanda J. Brown-Ramos, Monica Haytowitz, David B. Booth, Sarah L. TI Changes in the content and forms of vitamin K in processed foods SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Trans-fatty acids; Vitamin K; Dihydrophylloquinone; Phylloquinone; Food composition; Food analysis ID TRANS-FATTY-ACIDS; DIETARY TRANS; PHYLLOQUINONE; YOUNGER; ADULTS; WOMEN; OILS; MEN AB High intake of trans fatty acids has been linked to deleterious health effects. including increased risk of cardiovascular disease. Since 2006, the Food and Drug Administration requires companies to label the trans fatty acid content of foods. This has resulted in an overall decrease of commercially-hydrogenated oils in the food supply. Hydrogenation of vitamin K (VK)-rich plant oil changes the form and content of VK. It is not known if changes in use of hydrogenated oil in the U.S. food supply resulted in a change in the forms and amount of VK in processed foods. To test this, we compared 253 foods for total and individual forms of VK in foods analyzed pre- and post-2006 as part Of the U.S. Department of Agriculture (USDA) Nutrient and Food Analysis Program. Overall, foods identified as rich sources of the hydrogenated form of VK pre-2006 (dihydrophylloquinone; dK) had lower amounts of dK post-2006 with a concomitant increase in the parent form of VK, phylloquinone. However, the range of dK was large within foods, suggestive of a wide range of current practices regarding use of hydrogenated oils in the U.S. food supply. (C) 2015 Elsevier Inc. All rights reserved. C1 [Centi, Amanda J.; Brown-Ramos, Monica; Booth, Sarah L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Haytowitz, David B.] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. RP Booth, SL (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM sarah.booth@tufts.edu NR 17 TC 1 Z9 1 U1 1 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD AUG PY 2015 VL 41 BP 42 EP 44 DI 10.1016/j.jfca.2015.01.005 PG 3 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK4IP UT WOS:000356187800006 ER PT J AU Vanier, NL Paraginski, RT Berrios, JD Oliveira, LD Elias, MC AF Vanier, Nathan Levien Paraginski, Ricardo Tadeu Berrios, Jose De J. Oliveira, Leandro da Conceicao Elias, Moacir Cardoso TI Thiamine content and technological quality properties of parboiled rice treated with sodium bisulfite: Benefits and food safety risk SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Parboiling; Rice; Sodium bisulfite; Thiamine; Food processing; Nutrient retention/loss; Food safety; Food composition; Food analysis ID SULFUR-DIOXIDE; POSTHARVEST STORAGE; COLOR; DEFICIENCY; IMPACT; GRAIN; CATS; BRAN AB Rice is a staple food for more than half of the world's population. The parboiling process is known to increase the nutritional value of milled rice, but the process darkens the grains, with a corresponding negative effect on consumer acceptability. The aim of this study was to evaluate the effects of using different concentrations of sodium bisulfite (0, 0.2, 0.4, 0.6, 0.8, 1.0%) during the soaking step of rice parboiling process on the thiamine content and technological properties of parboiled rice. Moreover, the residual sulfite content in parboiled grains was also evaluated. The lowest concentration of 0.2% sodium bisulfite was able to significantly (p < 0.05) promote a whiter colour, a decrease in the percentage of stained grains, and an increase in the percentage of completely gelatinized grains in the parboiled rice, compared to rice without sodium bisulfite treatment. However, the use of sodium bisulfite significantly (p < 0.05) reduced the thiamine content, which is considered a risk associated with the treatment. (C) 2015 Elsevier Inc. All rights reserved. C1 [Vanier, Nathan Levien; Paraginski, Ricardo Tadeu; Elias, Moacir Cardoso] Univ Fed Pelotas, Dept Agroind Sci & Technol, BR-96160000 Pelotas, Brazil. [Vanier, Nathan Levien; Berrios, Jose De J.] ARS, Hlth Proc Foods Res Unit, WRRC, USDA, Albany, CA 94710 USA. [Oliveira, Leandro da Conceicao] Inst Fed Sul Riograndense, BR-96060290 Pelotas, Brazil. RP Vanier, NL (reprint author), Univ Fed Pelotas, Dept Agroind Sci & Technol, BR-96160000 Pelotas, Brazil. EM nathanvanier@hotmail.com OI Vanier, Nathan/0000-0001-6592-3023 NR 31 TC 0 Z9 0 U1 4 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD AUG PY 2015 VL 41 BP 98 EP 103 DI 10.1016/j.jfca.2015.02.008 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK4IP UT WOS:000356187800014 ER PT J AU Coyle, DR Klepzig, KD Koch, FH Morris, LA Nowak, JT Oak, SW Otrosina, WJ Smith, WD Gandhi, KJK AF Coyle, David R. Klepzig, Kier D. Koch, Frank H. Morris, Lawrence A. Nowak, John T. Oak, Steven W. Otrosina, William J. Smith, William D. Gandhi, Kamal J. K. TI A review of southern pine decline in North America SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Abiotic factors; Heterobasidion; Leptographium; Pinus taeda; Pinus palustris; Root herbivory ID SOUTHEASTERN UNITED-STATES; INHABITING OPHIOSTOMATOID FUNGI; SUMMER RAINFALL VARIABILITY; INTENSIVE FOREST MANAGEMENT; BARK BEETLE INFESTATIONS; LITTLELEAF DISEASE SITES; BLUE-STAIN FUNGI; LOBLOLLY-PINE; LONGLEAF PINE; TREE MORTALITY AB The southeastern United States is among the most productive forested areas in the world. Four endemic southern pine species - loblolly, longleaf, shortleaf, and slash contribute significantly to the economic and ecological values in the region. A recently described phenomenon known as Southern Pine Decline (SPD) has been reported as having widespread impact in the southern pine region, particularly on loblolly pine. Root-feeding weevils and their associated fungi have been suggested as causal agents, even though literature and empirical research suggests that they are secondary insects colonizing weakened trees. Further, no published information exists about whether their associated fungi can cause mortality of mature trees in the southeastern U.S. Since there are significant management implications for pine health, we reviewed and critically examined the SPD phenomena on the southern landscape. Our regional analyses of USDA Forest Inventory and Analysis data show no discernable patterns related to pine growth or mortality, especially as related to topographic factors. There are no large-scale patterns related to pine mortality suggesting multiple interacting factors impacting tree health at stand-level. As such, the hypothesis that SPD is a regionally important decline syndrome and labeling declining southern pine stands as SPD is not supported. Instead, we discuss many abiotic (soil types, climate) and biotic (insects, pathogens, genetics) factors that may be interacting with each other and affecting southern pine health. Finally, we suggest management recommendations for landowners with pine health issues. (C) 2015 Elsevier B.V. All rights reserved. C1 [Coyle, David R.; Morris, Lawrence A.; Gandhi, Kamal J. K.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Klepzig, Kier D.] US Forest Serv, USDA, Southern Res Stn, Asheville, NC USA. [Koch, Frank H.; Smith, William D.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC USA. [Nowak, John T.; Oak, Steven W.] US Forest Serv, USDA, Forest Hlth Protect, Asheville, NC USA. [Otrosina, William J.] US Forest Serv, USDA, Southern Res Stn, Athens, GA USA. RP Coyle, DR (reprint author), Univ Georgia, Warnell Sch Forestry & Nat Resources, Bldg 4-434,180 E Green St, Athens, GA 30602 USA. EM drcoyle@uga.edu OI Koch, Frank/0000-0002-3750-4507 FU USDA Forest Service; Forest Health Protection; USDA Forest Service Southern Research Station; Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia FX We dedicate this paper to the late Robert Mitchell (Joseph W. Jones Ecological Research Center, Georgia), who provided much intellectual impetus to work on pine health issues before his untimely death. We appreciate the thoughtful conversations with many people pertaining to this topic, including personnel at the Georgia Forestry Commission (Scott Cameron, Chip Bates, James Johnson), South Carolina Forestry Commission (Chisolm Beckham), Florida Forest Service (Jeff Eickwort), USDA Forest Service (Michelle Cram, Steve Fraedrich), Virginia Department of Forestry (Chris Asaro), and University of Georgia (Brittany Barnes). We thank Jim Worrall and Michelle Cram (USDA Forest Service, Forest Health Protection), Paul Hennon (USDA Forest Service, Pacific Northwest Research Station), Mike Ulyshen and Steve Fraedrich (USDA Forest Service, Southern Research Station) and two anonymous reviewers for reviewing earlier drafts of this manuscript. Danielle Sank (University of Georgia) assisted with formatting the paper. Funding was provided by the USDA Forest Service, Forest Health Protection, USDA Forest Service Southern Research Station, and Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia. NR 180 TC 3 Z9 3 U1 11 U2 57 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 1 PY 2015 VL 349 BP 134 EP 148 DI 10.1016/j.foreco.2015.04.007 PG 15 WC Forestry SC Forestry GA CK3MA UT WOS:000356119000015 ER PT J AU Fiuza, VRS Oliveira, FCR Fayer, R Santin, M AF Fiuza, V. R. S. Oliveira, F. C. R. Fayer, R. Santin, M. TI First report of Enterocytozoon bieneusi in pigs in Brazil SO PARASITOLOGY INTERNATIONAL LA English DT Article DE Brazil; Enterocytozoon bieneusi; Genotypes; PCR; Pigs ID VIRUS-INFECTED PATIENTS; EASTERN UNITED-STATES; MOLECULAR CHARACTERIZATION; ENCEPHALITOZOON-CUNICULI; STOOL SAMPLES; DAIRY-CATTLE; INTESTINAL MICROSPORIDIOSIS; GIARDIA-DUODENALIS; HOST-SPECIFICITY; HIGH PREVALENCE AB Although Brazil is the world's fourth largest producer and exporter of pork, there is no information on Enterocytozoon bieneusi in pigs. This study was undertaken to determine the presence of E. bieneusi in pigs in the State of Rio de Janeiro, Brazil. Fecal samples were collected from 91 pigs (1- to 12-month-old) in 10 properties and examined by molecular methods. The presence of E. bieneusi was determined by PCR and all PCR positive specimens were sequenced to determine the genotype by nucleotide sequence analysis of the internal transcribed spacer of the rRNA gene. E. bieneusi was found in pigs in all farms. Fifty four (59.3%) samples were E. bieneusi-positive. A wide genetic diversity was found with 21 genotypes identified, 4 previously reported (0, EbpA, CS-1, and H) and 17 novel genotypes named PigEb1-PigEb17. All 17 novel genotypes identified in this study clustered within the previously designated zoonotic Group 1. The most prevalent genotypes were novel genotypes PigEb2 and PigEb4 (16/91, 17.6%, each). Mixed infections with 2 or 3 genotypes were detected in 13 pigs (24.1%). The high prevalence in pigs observed in this study, the description of two known zoonotic genotypes (EbpA and O), and the report of 17 new genotypes of E. bieneusi, represent an important advancement in the study of the wide genetic diversity of this organism, emphasizing the importance of further research, especially in geographical areas where little or no research has been conducted. The zoonotic risk of these novel genotypes and their importance to other animal species is still unknown, but needs to be further evaluated. Published by Elsevier Ireland Ltd. C1 [Fiuza, V. R. S.; Fayer, R.; Santin, M.] ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, Beltsville, MD 20705 USA. [Fiuza, V. R. S.] Minist Educ Brazil, CAPES Fdn, BR-70040020 Brasilia, DF, Brazil. [Oliveira, F. C. R.] Univ Estadual Norte Fluminense, BR-28013602 Rio De Janeiro, Brazil. RP Santin, M (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, Bldg 173,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM monica.santin-duran@ars.usda.gov FU Ministry of Education of Brazil [BEX 2705-13-3] FX This study was supported by the Ministry of Education of Brazil, process no BEX 2705-13-3. NR 86 TC 9 Z9 9 U1 1 U2 5 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 1383-5769 J9 PARASITOL INT JI Parasitol. Int. PD AUG PY 2015 VL 64 IS 4 BP 18 EP 23 DI 10.1016/j.parint.2015.01.002 PG 6 WC Parasitology SC Parasitology GA CK3LA UT WOS:000356116400003 PM 25582928 ER PT J AU Funk, PA Elsayed, K Yeater, KM Holt, GA Whitelock, DP AF Funk, P. A. Elsayed, K. Yeater, K. M. Holt, G. A. Whitelock, D. P. TI Could cyclone performance improve with reduced inlet velocity? SO POWDER TECHNOLOGY LA English DT Article DE Cyclones; Emissions; Energy consumption; Fine particulate; PM2.5 ID PRESSURE-DROP; 1D3D CYCLONES; DESIGN; FLOW; GAS; EFFICIENCY; EMISSIONS; DIAMETER AB Emission abatement cyclone performance is improved by increasing collection effectiveness or decreasing energy consumption. The object of this study was to quantify the pressure drop and fine particulate (PM2.5) collection of 1D3D cyclones (H = 4Dc, h = 1Dc) at inlet velocities from 8 to 18 m s(-1) (Stk = 0.7-1.5) using heterogeneous particulate as a test material at inlet concentrations from 3 to 75 g m(-3). Cyclone exhaust was passed through filters. Laser diffraction particle size distribution analysis was used to estimate PM2.5 emissions. Response surface models showed a strong correlation between cyclone pressure loss (Euler number) and inlet velocity and predicted a 46% reduction in pressure loss for a 25% reduction in inlet velocity (Stokes number). The model for PM2.5 emissions was less definitive and, surprisingly, predicted a 31% decrease in PM2.5 emissions when operating 25% below the design inlet velocity. Operating below the design inlet velocity (at a lower Stokes number) to reduce pressure losses (Euler number) would reduce both the financial and the environmental cost of procuring electricity. The unexpected co-benefit suggested by these trials was that emission abatement may improve at the same time, though other empirical trials have shown emissions to be independent of inlet velocity and Stokes number. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licensesiby-nc-nd/4.0/). C1 [Funk, P. A.; Whitelock, D. P.] USDA ARS, Southwestern Cotton Ginning Res Lab, Mesilla Pk, NM USA. [Elsayed, K.] Helwan Univ, Dept Power Mech Engn, Cairo, Egypt. [Yeater, K. M.] USDA ARS, Plains Area, Ft Collins, CO 80522 USA. [Holt, G. A.] USDA ARS, Cotton Prod & Proc Res Unit, Lubbock, TX USA. RP Funk, PA (reprint author), POB 578, Mesilla Pk, NM 88047 USA. EM paul.funk@ars.usda.gov RI Elsayed, khairy/A-2462-2011; Elsayed, Khairy/O-6781-2016 OI Elsayed, khairy/0000-0002-3157-327X; Elsayed, Khairy/0000-0002-3157-327X NR 36 TC 1 Z9 1 U1 2 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0032-5910 EI 1873-328X J9 POWDER TECHNOL JI Powder Technol. PD AUG PY 2015 VL 280 BP 211 EP 218 DI 10.1016/j.powtec.2015.04.026 PG 8 WC Engineering, Chemical SC Engineering GA CK4QQ UT WOS:000356208700024 ER PT J AU Horvitz, CC Koop, AL Erickson, KD AF Horvitz, Carol C. Koop, Anthony L. Erickson, Kelley D. TI TIME-INVARIANT AND STOCHASTIC DISPERSER-STRUCTURED MATRIX MODELS: INVASION RATES OF FLESHY-FRUITED EXOTIC SHRUBS SO DISCRETE AND CONTINUOUS DYNAMICAL SYSTEMS-SERIES B LA English DT Article DE Ardisia elliptica; biological invasions; dispersal kernel; integrodifference equation; invasion rate; matrix models; Everglades National Park; Florida ID PLANT MIGRATION RATES; SEED DISPERSAL; SENSITIVITY-ANALYSIS; ARDISIA-ELLIPTICA; DEMOGRAPHY; FORESTS; SPREAD AB Interest in spatial population dynamics includes applications to the spread of disease and invasive species. Recently, models for structured populations have been extended to incorporate temporal variation in both demography and dispersal. Here we propose a novel version of the model that incorporates structured dispersal to evaluate how changes in the relative proportion of mammalian, and short- and long-distance avian dispersers affect the rate of spread of an invasive shrub, Ardisia elliptica in Everglades National Park. We implemented 45 time-invariant models, including one in which a single dispersal kernel was estimated from field data by pooling all seedlings, and 44 that were disperser-structured in which dispersal kernels were estimated separately for gravity-, catbird-, robin- and raccoon-dispersed seed. Robins, the longest distance dispersers, are infrequent. Finally we implemented a time-varying model that included variability among years in the proportion of seeds that were taken by robins. The models estimated invasion speeds that ranged from 3.9 to 34.7 m yr(-1). Infrequent long-distance dispersal by robins were important in determining invasion speed in the disperser-structured model. Comparing model projections with the (historically) known rate of spread, we show how a model that stratifies seeds by dispersal agents does better than one that ignores them, although all of our models underestimate it. C1 [Horvitz, Carol C.; Erickson, Kelley D.] Univ Miami, Dept Biol, Inst Theoret & Math Ecol, Coral Gables, FL 33124 USA. [Koop, Anthony L.] USDA, Plant Protect & Quarantine, Plant Epidemiol & Risk Anal Lab, Raleigh, NC 27606 USA. RP Horvitz, CC (reprint author), Univ Miami, Dept Biol, Inst Theoret & Math Ecol, POB 249118, Coral Gables, FL 33124 USA. EM carolhorvitz@miami.edu; anthony.l.koop@aphis.usda.gov; kerickson22@bio.miami.edu FU Department of Biology, University of Miami FX We thank Steve Cantrell and Chris Cosner for inviting our participation in the Everything Disperses to Miami conference held by the Institute of Theoretical and Mathematical Ecology at the University of Miami in December 2012, where we presented many of these results. We thank Michael Neubert and Hal Caswell for inviting participation in the NCEAS Demography and Dispersal Synthesis Working Group (2001-2002). This field research and earlier versions of the time-invariant models directly resulted from the working group; MATLAB code for the analyses of wave speed that formed the basis of code used here were developed for and by the working group. Also, group participants James Clark, Janneke HilleRisLambers and Brian Beckage provided S-PLUS code to estimate dispersal kernels from mapped seedlings and adults. We thank Ecology 236 students at University of Miami and Justin Nigham, Fernando Valido, Agda Barreirinhas and Marcelo Tamasia for field assistance, and Everglades National Park for research permits (No: 1999125 and 2000107), and the Department of Biology, University of Miami for financial support. We thank Eelke Jongejians for comments on a previous version of this ms. This is Contribution No. XXX of the Program in Tropical Biology, Conservation and Behavior at the University of Miami. NR 31 TC 0 Z9 0 U1 5 U2 28 PU AMER INST MATHEMATICAL SCIENCES-AIMS PI SPRINGFIELD PA PO BOX 2604, SPRINGFIELD, MO 65801-2604 USA SN 1531-3492 EI 1553-524X J9 DISCRETE CONT DYN-B JI Discrete Contin. Dyn. Syst.-Ser. B PD AUG PY 2015 VL 20 IS 6 SI SI BP 1639 EP 1662 DI 10.3934/dcdsb.2015.20.1639 PG 24 WC Mathematics, Applied SC Mathematics GA CJ5YQ UT WOS:000355570500006 ER PT J AU Brauer, N Maynard, JJ Dahlgren, RA O'Geen, AT AF Brauer, Neil Maynard, Jonathan J. Dahlgren, Randy A. O'Geen, Anthony T. TI Fate of nitrate in seepage from a restored wetland receiving agricultural tailwater SO ECOLOGICAL ENGINEERING LA English DT Article DE Agricultural runoff; Wetlands; Nonpoint source pollution; Nitrogen; Nitrate leaching ID WATER-TREATMENT WETLAND; CONSTRUCTED WETLANDS; WASTE-WATER; ORGANIC-MATTER; DENITRIFICATION RATES; NATURAL WETLANDS; DEPTH FUNCTIONS; NORTH-CAROLINA; TILE DRAINAGE; NITROGEN AB Constructed and restored wetlands are a common practice to filter agricultural runoff, which often contains high levels of pollutants, including nitrate. Seepage waters from wetlands have potential to contaminate groundwater. This study used soil and water monitoring and hydrologic and nitrogen mass balances to document the fate and transport of nitrate in seepage and surface waters from a restored flow-through wetland adjacent to the San Joaquin River, California. A 39% reduction in NO3-N concentration was observed between wetland surface water inflows (12.87 +/- 6.43 mg L-1; mean +/- SD) and outflows (7.87 +/- 4.69 mg L-1). Redox potentials were consistently below the nitrate reduction threshold (similar to 250 mV) at most sites throughout the irrigation season. In the upper 10 cm of the main flowpath, denitrification potential (DNP) for soil incubations significantly increased from 151 to 2437 mg NO3-N m(-2) d(-1) when nitrate was added, but showed no response to carbon additions indicating that denitrification was primarily limited by nitrate. Approximately 72% of the water entering the wetland became deep seepage, water that percolated beyond 1-m depth. The wetland was highly effective at removing nitrate (3866 kg NO3-N) with an estimated 75% NO3-N removal efficiency calculated from a combined water and nitrate mass balance. The mass balance results were consistent with estimates of NO3-N removed (5085 kg NO3-N) via denitrification potential. Results indicate that allowing seepage from wetlands does not necessarily pose an appreciable risk for groundwater nitrate contamination and seepage can facilitate greater nitrate removal via denitrification in soil compared to surface water transport alone. (C) 2015 Elsevier B.V. All rights reserved. C1 [Brauer, Neil; Dahlgren, Randy A.; O'Geen, Anthony T.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Maynard, Jonathan J.] New Mexico State Univ, USDA ARS, Jornada Expt Range, Las Cruces, NM 88003 USA. RP O'Geen, AT (reprint author), Univ Calif Davis, Dept Land Air & Water Resources, One Shields Ave, Davis, CA 95616 USA. EM atogeen@ucdavis.edu FU California State Water Resources Control Board [05-055-555-0] FX Funding for this project has been provided in part through an agreement with the California State Water Resources Control Board, Grant Agreement No. 05-055-555-0. The contents of this document do not necessarily reflect the views and policies of the California State Water Resources Control Board, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. We gratefully acknowledge the assistance of Jiayou Deng, Tony Orozco, James Chang, Jeannie Evatt, and Max Edwards. NR 56 TC 3 Z9 3 U1 8 U2 55 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD AUG PY 2015 VL 81 BP 207 EP 217 DI 10.1016/j.ecoleng.2015.04.003 PG 11 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA CJ7KC UT WOS:000355672800026 ER PT J AU Stottlemyer, AD Waldrop, TA Wang, GG AF Stottlemyer, Aaron D. Waldrop, Thomas A. Wang, G. Geoff TI Prescribed burning and mastication effects on surface fuels in southern pine beetle-killed loblolly pine plantations SO ECOLOGICAL ENGINEERING LA English DT Article DE Forest disturbance; Site preparation; Hazard reduction; Fire behavior; Masticated debris ID PREDICTED FIRE BEHAVIOR; MIXED-CONIFER FOREST; MOUNTAIN PINE; APPALACHIAN MOUNTAINS; LODGEPOLE PINE; REDUCTION TREATMENTS; MECHANICAL MASTICATION; VEGETATION RESPONSE; MOISTURE-CONTENT; OAK FORESTS AB Surface fuels were characterized in loblolly pine (Pinus taeda L.) plantations severely impacted by southern pine beetle (Dendroctonus frontalis Ehrh.) (SPB) outbreaks in the upper South Carolina Piedmont. Prescribed burning and mastication were then tested as fuel reduction treatments in these areas. Prescribed burning reduced fuelbed continuity by consuming litter (Oi layer), duff (Oe + Oa), and woody surface fuels (1-, 10-, and 100-h timelag size classes) immediately after the treatment. Total loading of 1- and 10-h fuels in burned stands (3.1 Mg ha (1)) remained significantly lower than that in the control (no treatment) (5.6 Mg ha (1)) in the 2nd year post-treatment. However, 100- and 1000-h fuels increased post-burn due to accelerated failure of remaining pine snags and totaled 14.5 Mg ha (1) in the 2nd year post-treatment which was not significantly different than the control (17.3 Mg ha (1)). Mineral soil exposure averaged 73% of burned stands after consumption of the duff layer in many areas. Custom low, moderate, and high load fuel models were developed for SPB-killed stands and produced simulated fire behavior (flame length and rate of spread) similar to two standard slash-blowdown fuel models (SB2 and SB3) when input to the BehavePlus fire modeling system. Mastication resulted in a compacted (bulk density = 131.3 kg m (3)) and continuous layer of woody debris that averaged 15.1 cm in depth. Equations were developed for estimating masticated debris load and utilize fuelbed depth as input. The masticated debris load averaged 192.4 Mg ha (1) in the 1st year post-treatment and was significantly higher than total fuel loading in burned (16.3 Mg ha (1)) and control (24.3 Mg ha (1)) stands. The treatments tested in this study provide different options for preparing SPB-killed areas for reforestation activities and may produce short-term reductions in fire hazard. (C) 2015 Elsevier B.V. All rights reserved. C1 [Stottlemyer, Aaron D.] Penn State Univ, Du Bois, PA 15801 USA. [Waldrop, Thomas A.] USDA Forest Serv, Southern Res Stn, Clemson, SC 29631 USA. [Wang, G. Geoff] Clemson Univ, Dept Forestry & Nat Resources, Clemson, SC 29631 USA. RP Stottlemyer, AD (reprint author), Penn State Univ, One Coll Pl, Du Bois, PA 15801 USA. EM ads175@psu.edu FU Joint Fire Science Program [JFSP 04-2-1-33] FX We acknowledge funding from the Joint Fire Science Program under Project JFSP 04-2-1-33. We greatly appreciate field assistance from Will Faulkner, Richard Layton, Jay Garcia, Andy Nuffer, Ross Phillips, Helen Mohr, Greg Chapman, Chuck Flint, Lucy Brudnak, Mitch Smith, and Eddie Gambrel. Support with site selection and treatment implementation from Knight Cox and Bryan Odom with the Clemson Experimental Forest was critical to the completion of this study. Rob Loeb and two anonymous reviewers provided constructive comments on an earlier version of the manuscript. NR 68 TC 2 Z9 2 U1 4 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD AUG PY 2015 VL 81 BP 514 EP 524 DI 10.1016/j.ecoleng.2015.04.076 PG 11 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA CJ7KC UT WOS:000355672800060 ER PT J AU Farrow, EM Wang, JM Burken, JG Shi, HL Yan, WG Yang, J Hua, B Deng, BL AF Farrow, Eric M. Wang, Jianmin Burken, Joel G. Shi, Honglan Yan, Wengui Yang, John Hua, Bin Deng, Baolin TI Reducing arsenic accumulation in rice grain through iron oxide amendment SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Rice cultivar; Grain accumulation; Arsenic; Soil; Iron oxide ID CULTIVARS; PHOSPHATE; SOIL; AVAILABILITY; LEAD AB Effects of soil-arsenic (As), phosphorus and iron oxide on As accumulation in rice grain were investigated. Cultivars that have significantly different sensitivity to As, straighthead-resistant Zhe 733 and straighthead-susceptible Cocodrie, were used to represent different cultivar varieties. The grain accumulation of other elements of concern, selenium (Se), molybdenum (Mo), and cadmium (Cd) was also monitored. Results demonstrated that high soil-As not only resulted in high grain-As, but could also result in high grain-Se, and Zhe 733 had significantly less grain-As than Cocodrie did. However, soil-As did not impact grain-Mo and Cd. Among all elements monitored, iron oxide amendment significantly reduced grain-As for both cultivars, while the phosphate application only reduced grain-Se for Zhe 733. Results also indicated that cultivar type significantly impacted grain accumulation of all monitored trace elements. Therefore, applying iron oxide to As-contaminated land, in addition to choosing appropriate rice cultivar, can effectively reduce the grain accumulation of As. (C) 2015 Elsevier Inc. All rights reserved. C1 [Farrow, Eric M.; Wang, Jianmin; Burken, Joel G.] Missouri Univ Sci & Technol, Dept Civil Architectural & Environm Engn, Rolla, MO 65409 USA. [Shi, Honglan] Missouri Univ Sci & Technol, Dept Chem, Rolla, MO USA. [Yan, Wengui] USDA, Agr Res Serv, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Yang, John; Hua, Bin] Lincoln Univ Missouri, Dept Agr & Environm Sci, Jefferson City, MO USA. [Deng, Baolin] Univ Missouri, Dept Civil & Environm Engn, Columbia, MO USA. RP Wang, JM (reprint author), Missouri Univ Sci & Technol, Dept Civil Architectural & Environm Engn, Rolla, MO 65409 USA. EM wangjia@mst.edu RI Burken, Joel /C-2099-2016 OI Burken, Joel /0000-0002-7774-5364 FU U.S. Department of Agriculture [2008-38814-04727] FX This research was funded by U.S. Department of Agriculture (Award no. 2008-38814-04727) to Missouri University of Science & Technology through Lincoln University of Missouri. The authors would like to thank Ms. Tiffany Sookaserm for sample collection and preparation, Ms. Brandi Clark for assistance in method development, and Prof. V. A. Samaranayake for assistance with statistical analysis. NR 27 TC 3 Z9 3 U1 11 U2 73 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD AUG PY 2015 VL 118 BP 55 EP 61 DI 10.1016/j.ecoenv.2015.04.014 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA CJ2ZQ UT WOS:000355353600007 PM 25910688 ER PT J AU Forcella, F Eberle, CA Gesch, RW Johnson, JMF AF Forcella, Frank Eberle, Carrie A. Gesch, Russ W. Johnson, Jane M. F. TI Oilseed cuphea tolerates bromoxynil SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Cuphea viscosissima Jacq. X C.; Cuphea lanceolata WT Aiton 'PSR23'; Herbicide tolerance; Weed management ID NORTHERN CORN-BELT; SEED YIELD; OIL; HERBICIDES AB Weed management is a critical feature of all crop production, but especially for new and alternative crops with which most growers have little experience. Oilseed cuphea is a new annual crop for temperate regions and, at present, it is known to tolerate only a narrow spectrum of herbicides. Additional herbicides would be useful to encourage growers to examine this crop on their farms. Of particular importance are herbicides with broad activity against dicotyledonous weeds. Accordingly, cuphea tolerance to bromoxynil was tested in both greenhouse and field settings. Oilseed cuphea tolerated postemergence applications of bromoxynil up to 210 g ae ha(-1), which is a common label rate for this herbicide for traditional crops, but only if the plants had reached the 4- to 5-leaf stage of development at the time of application. Earlier applications, or applications at higher rates, could damage plants or reduce stands. Thus, postemergence applications of bromoxynil at or beyond the 5-leaf stage appear safe cuphea. This result broadens the spectrum of potentially competing weed species that can be managed effectively if they co-occur with oilseed cuphea. Published by Elsevier B.V. C1 [Forcella, Frank; Eberle, Carrie A.; Gesch, Russ W.; Johnson, Jane M. F.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Forcella, F (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM frank.forcella@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 FU Aveda Corporation FX Our general research on cuphea management was funded in part by Aveda Corporation. Managers of the Swan Lake Research Farm, Charles Hennen and Scott Larson, as well as Gary Amundson, Dean Peterson, and James Eklund have been instrumental in the success of the cuphea project. NR 16 TC 0 Z9 0 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD AUG PY 2015 VL 70 BP 201 EP 203 DI 10.1016/j.indcrop.2015.03.061 PG 3 WC Agricultural Engineering; Agronomy SC Agriculture GA CJ7VS UT WOS:000355709100026 ER PT J AU Pothula, AK Igathinathane, C Kronberg, S AF Pothula, Anand Kumar Igathinathane, C. Kronberg, S. TI Profile based image analysis for identification of chopped biomass stem nodes and internodes SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biofuel; Bioproducts; Feedstock quality; ImageJ; Separation; Sorting ID PARTICLE-SIZE DISTRIBUTION; FOOD QUALITY EVALUATION; CORN STOVER; MECHANICAL ENERGY; COMPUTER VISION; WHEAT-STRAW; SWITCHGRASS; ALGORITHM; KERNELS; SHAPE AB Because of their significant variation in chemical composition, segregation of chopped biomass into nodes and internodes helps in efficient utilization of these feedstocks. Stem internodes having low ash content are a better feedstock for biofuel and bioenergy applications than nodes. However, separation of these components is challenging because their physical characteristics are similar. We applied an image processing technique to identify nodes and internodes of chopped biomass from scanned digital images. In this study, we utilized the object profile identified differences in the node and intemode components and tested on chopped corn stalks and switchgrass stems. We considered four methods of image processing including rectangularity, solidity, width-, and slope-variation and developed an ImageJ plugin for the node-internode identification. Digital chopping of the ends of the objects was necessary for identification, especially dealing with projecting fibers and chipped rough ends, and an algorithm was developed for this. Among the methods tested, width-variation gave the best identification accuracy (97-98%), followed by rectangularity (93-96%), solidity (86-91%), and slope-variation (69-82%). Rectangularity - a relatively simpler method, and solidity - a standard ImageJ output, can be directly used to perform identification. The developed approach of node-internode identification can be easily applied to other chopped biomass and similar materials, and its application may lead to efficient biomass end use in biofuel and bioproduct industries. Published by Elsevier B.V. C1 [Pothula, Anand Kumar; Igathinathane, C.] N Dakota State Univ, Dept Agr & Biosyst Engn, Fargo, ND 58102 USA. [Kronberg, S.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Igathinathane, C (reprint author), N Dakota State Univ, Dept Agr & Biosyst Engn, 1221 Albrecht Blvd, Fargo, ND 58102 USA. EM lgathinathane.Cannayen@ndsu.edu OI Pothula, Anand/0000-0002-6129-5194 FU Renewable Energy Program of North Dakota Industrial Commission, Bismarck, ND [FAR0016577]; USDA - Agricultural Research Service [FAR0020464] FX The Renewable Energy Program (Grant number: FAR0016577, 2010) of North Dakota Industrial Commission, Bismarck, ND, and the USDA - Agricultural Research Service (Grant number: FAR0020464, 2012) provided funding for this research and these supports are gratefully appreciated. NR 32 TC 0 Z9 0 U1 3 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD AUG PY 2015 VL 70 BP 374 EP 382 DI 10.1016/j.indcrop.2015.03.048 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA CJ7VS UT WOS:000355709100048 ER PT J AU Rasutis, D Soratana, K McMahan, C Landis, AE AF Rasutis, Daina Soratana, Kullapa McMahan, Colleen Landis, Amy E. TI A sustainability review of domestic rubber from the guayule plant SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Review DE Guayule; Natural rubber; Hevea; Sustainability; Life cycle assessment ID NATURAL-RUBBER; SOUTHERN THAILAND; SEED OIL; BIODIESEL PRODUCTION; HEVEA-BRASILIENSIS; PARTHENIUM-ARGENTATUM; ALTERNATIVE SOURCES; LATEX ALLERGY; WASTE-WATER; RESIN AB Guayule (Parthenium argentatum Gray) is an arid-adapted, low-input perennial shrub native to Mexico and southern Texas that has received considerable attention as an alternative source of natural rubber. It has potential to replace the most common types of rubbers, including synthetic rubber derived from petroleum and natural rubber, which is tapped from Hevea (Hevea brasiliensis) trees grown in tropical regions, primarily Southeast Asia. The guayule plant produces natural rubber in its bark parenchyma cells and the shrub is processed to extract the latex. Guayule rubber is comparable in quality to Hevea natural rubber and the residual, non-latex guayule plant material can be transformed into valuable co-products, such as bioenergy. This review introduces the reader to guayule rubber production (agriculture, processing and products) and explores the sustainability implications of guayule rubber commercialization related to the three pillars of sustainability, including environmental impacts of rubber production, economic barriers and advantages, and social implications. The review highlights areas of focus that could be leveraged to help guayule become a more sustainable source of natural rubber. Guayule rubber provides an opportunity to lower the environmental impacts of a major commodity, to develop an industry to support the local U.S, economy, and to reduce U.S. dependence on non-renewable petroleum sources and rubber imports. Proposed recommendations to further support guayule sustainability include improving the efficiency of agricultural and processing activities, utilization of guayule co-products to improve economics of guayule cultivation, and the establishment of a secure guayule rubber supply at a production level that could consistently meet rubber demands. A better understanding of guayule rubber life-cycle impacts could be a way to reduce the environmental footprint of guayule rubber products and expedite its commercialization. (C) 2015 Elsevier B.V. All rights reserved. C1 [Rasutis, Daina; Soratana, Kullapa; Landis, Amy E.] Arizona State Univ, Sch Sustainable Engn & Built Environm, Tempe, AZ 85281 USA. [Soratana, Kullapa] Naresuan Univ, Sch Logist & Supply Chain, Muang 65000, Phitsanulok, Thailand. [McMahan, Colleen] ARS, USDA, Western Reg Res Lab, Berkeley, CA USA. RP Landis, AE (reprint author), Arizona State Univ, Sch Sustainable Engn & Built Environm, 781 E Terrace Rd, Tempe, AZ 85281 USA. EM amy.landis@asu.edu FU USDA Biomass Research and Development Initiative (BRDI) (USDA-NIFA) [2012-10006-19391 OH] FX This work was supported by the USDA Biomass Research and Development Initiative (BRDI) grant (USDA-NIFA 2012-10006-19391 OH). Any opinions, findings and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of USDA or other participants in the grant. NR 141 TC 7 Z9 7 U1 11 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD AUG PY 2015 VL 70 BP 383 EP 394 DI 10.1016/j.indcrop.2015.03.042 PG 12 WC Agricultural Engineering; Agronomy SC Agriculture GA CJ7VS UT WOS:000355709100049 ER PT J AU Zieman, EA Reeve, JD Braswell, WE Jimenez, FA AF Zieman, Elliott A. Reeve, John D. Braswell, W. Evan Jimenez, F. Agustin TI Pathology, distribution, morphological and genetic identity of Deladenus proximus (Tylenchida: Neotylenchidae) a parasitic nematode of the woodwasp, Sirex nigricornis in the eastern United States SO BIOLOGICAL CONTROL LA English DT Article DE Amylostereum; Biological control; Parasite induced sterilization; Woodwasps; Entomopathogenic nematode ID ROT FUNGUS AMYLOSTEREUM; BIOLOGICAL-CONTROL; SIRICID WOODWASPS; HYMENOPTERA-SIRICIDAE; NOCTILIO HYMENOPTERA; NORTH-AMERICA; AREOLATUM; HEMISPHERE; STRAINS; ECOLOGY AB The woodwasp, Sirex nigricornis (Hymenoptera: Siricidae), is solitary and utilizes a symbiotic fungus to extract nourishment from pine trees to feed its larvae. The woodwasp has a brief adult life, but the larvae develop for 1-3 years in the tree xylem. Infections with the nematode Deladenus proximus have been documented in the native woodwasp, Sirex nigricornis in the eastern United States and Canada. These nematodes appear to sterilize female woodwasps; however, the extent of the pathology and other aspects of the biology of Deladenus proximus remain unknown. In this study we examined the effects of Deladenus proximus on Sirex nigricornis using fresh - not preserved - specimens. Between 2009 and 2012, a total of 1639 woodwasps were examined for internal nematodes from emerging sites in Illinois, Louisiana and South Carolina. From this total, only 112 individuals were infected with the nematode Deladenus proximus, with varying prevalence across localities and years. Nematodes were found inside every egg of infected females, as well as the hemocoel and the mycangia. Morphometric analyses of mycetophagous reared adult nematodes suggest that a single species is present in localities from Arkansas, Illinois, Louisiana, New York and South Carolina. The screening of the mitochondrial cytochrome oxidase subunit 1 (cox1) of these organisms is consistent with this pattern in that all of these individuals belong to a single clade. Deladenus proximus appears to be an efficient sterilizer, yet its prevalence is relatively low. Experimental infections of the invasive Sirex noctilio are recommended to test the viability of using this nematode as a biological control agent. (C) 2015 The Authors. Published by Elsevier Inc. C1 [Zieman, Elliott A.; Reeve, John D.; Jimenez, F. Agustin] So Illinois Univ, Dept Zool, Carbondale, IL 62901 USA. [Braswell, W. Evan] USDA APHIS, Mission Lab, Ctr Sci & Technol, Edinburg, TX 78541 USA. RP Jimenez, FA (reprint author), So Illinois Univ, Dept Zool, Carbondale, IL 62901 USA. EM agustinjz@zoology.siu.edu RI Jimenez, Francisco/I-6010-2013 FU US Forest Service [USDA/FS-09-CA-11420004-290]; USDA-APHIS [12-8130-0171-CA] FX This project was funded by the US Forest Service (USDA/FS-09-CA-11420004-290) and USDA-APHIS (12-8130-0171-CA). Several collaborators facilitated specimens used in the completion of this project including Kevin J. Dodds and James Meeker (USDA-Forest Service); Scott Gardner and Gabor Racz (Harold W. Manter Laboratory Parasitological Collection, University of Nebraska); Jessica Hartshorn (University of Arkansas), and E. Erin Morris (Cornell University). Russell Bulluck, David Williams, Matthew Ciomperlik, Fred M. Stephen and an anonymous reviewer provided an insightful review to an earlier version of this manuscript. NR 49 TC 2 Z9 2 U1 2 U2 19 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD AUG PY 2015 VL 87 BP 14 EP 22 DI 10.1016/j.biocontrol.2015.04.009 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CJ1BG UT WOS:000355215900003 ER PT J AU Saha, BC Qureshi, N Kennedy, GJ Cotta, MA AF Saha, Badal C. Qureshi, Nasib Kennedy, Gregory J. Cotta, Michael A. TI Enhancement of xylose utilization from corn stover by a recombinant Escherichia coli strain for ethanol production SO BIORESOURCE TECHNOLOGY LA English DT Article DE Ethanol; Corn stover; Simultaneous saccharification and fermentation; Recombinant ethanologenic Escherichia coli; Substrate selective inoculum ID DILUTE PHOSPHORIC-ACID; ENZYMATIC SACCHARIFICATION; SACCHAROMYCES-CEREVISIAE; WHEAT-STRAW; FERMENTATION; PRETREATMENT; HYDROLYSIS; GLUCOSE; FBR5; FIBER AB Effects of substrate-selective inoculum prepared by growing on glucose, xylose, arabinose, GXA (glucose, xylose, arabinose, 1:1:1) and corn stover hydrolyzate (dilute acid pretreated and enzymatically hydrolyzed, CSH) on ethanol production from CSH by a mixed sugar utilizing recombinant Escherichia coli (strain FBR5) were investigated. The initial ethanol productivity was faster for the seed grown on xylose followed by GXA, CSH, glucose and arabinose. Arabinose grown seed took the longest time to complete the fermentation. Delayed saccharifying enzyme addition in simultaneous saccharification and fermentation of dilute acid pretreated CS by the recombinant E. coli strain FBR5 allowed the fermentation to finish in a shorter time than adding the enzyme simultaneously with xylose grown inoculum. Use of substrate selective inoculum and fermenting pentose sugars first under glucose limited condition helped to alleviate the catabolite repression of the recombinant bacterium on ethanol production from lignocellulosic hydrolyzate. Published by Elsevier Ltd. C1 [Saha, Badal C.; Qureshi, Nasib; Kennedy, Gregory J.; Cotta, Michael A.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Saha, BC (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Badal.Saha@ars.usda.gov NR 28 TC 6 Z9 6 U1 3 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD AUG PY 2015 VL 190 BP 182 EP 188 DI 10.1016/j.biortech.2015.04.079 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CJ0WN UT WOS:000355200200024 PM 25958140 ER PT J AU Cao, GL Ximenes, E Nichols, NN Frazer, SE Kim, D Cotta, MA Ladisch, M AF Cao, Guangli Ximenes, Eduardo Nichols, Nancy N. Frazer, Sarah E. Kim, Daehwan Cotta, Michael A. Ladisch, Michael TI Bioabatement with hemicellulase supplementation to reduce enzymatic hydrolysis inhibitors SO BIORESOURCE TECHNOLOGY LA English DT Article DE Bioabatement; Inhibitors; Hemicellulase supplementation; Enzymatic hydrolysis; Liquid hot water biomass pretreatment ID HOT-WATER PRETREATMENT; CORN STOVER; BIOLOGICAL ABATEMENT; XYLANASE; FERMENTATION; CELLULASES; CELLULOSE; ETHANOL; ENZYMES; PHENOLS AB A stepwise removal of inhibitory compounds by bioabatement combined with hemicellulase supplementation was conducted to enhance cellulose hydrolysis of liquid hot water-pretreated corn stover. Results showed that the fungus Coniochaeta ligniaria NRRL30616 eliminated most of the enzyme and fermentation inhibitors from liquid hot water-pretreated corn stover hydrolysates. Moreover, addition of hemicellulases after bioabatement and before enzymatic hydrolysis of cellulose achieved 20% higher glucose yields compared to non-treated samples. This work presents the mechanisms by which supplementation of the fungus with hemicellulase enzymes enables maximal conversion, and confirms the inhibitory effect of xylo-oligosaccharides in corn stover hydrolysates once the dominant inhibitory effect of phenolic compounds is removed. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Cao, Guangli; Ximenes, Eduardo; Kim, Daehwan; Ladisch, Michael] Purdue Univ, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA. [Nichols, Nancy N.; Frazer, Sarah E.; Cotta, Michael A.] USDA, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Agr Res Serv, Peoria, IL 61604 USA. [Cao, Guangli] Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China. [Cao, Guangli] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150001, Peoples R China. RP Ladisch, M (reprint author), Purdue Univ, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA. EM ladisch@purdue.edu OI Ximenes, Eduardo/0000-0001-9087-0218 NR 18 TC 7 Z9 7 U1 10 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD AUG PY 2015 VL 190 BP 412 EP 415 DI 10.1016/j.biortech.2015.04.064 PG 4 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CJ0WN UT WOS:000355200200055 PM 25958134 ER PT J AU Duan, Q Tait, RG Schneider, MJ Beitz, DC Wheeler, TL Shackelford, SD Cundiff, LV Reecy, JM AF Duan, Q. Tait, R. G., Jr. Schneider, M. J. Beitz, D. C. Wheeler, T. L. Shackelford, S. D. Cundiff, L. V. Reecy, J. M. TI Sire breed effect on beef longissimus mineral concentrations and their relationships with carcass and palatability traits SO MEAT SCIENCE LA English DT Article DE Beef; Breed; Longissimus; Iron; Magnesium; Zinc ID INSULIN-RESISTANCE; NONGENETIC FACTORS; MAGNESIUM INTAKE; ANIMAL-TISSUES; ANGUS CATTLE; IRON CONTENT; MUSCLE; COLOR; ASSOCIATIONS; MODEL AB The objective of this study was to evaluate sire breed effect on mineral concentration in beef longissimus thoracis (LT) and investigate the correlations between beef mineral concentrations and carcass and palatability traits. Steer progeny (N = 246) from the Germplasm Evaluation project Cycle VIII were used in this study. In addition to carcass traits, LT was evaluated for mineral concentrations, Warner-Bratzler shear force, and palatability traits. A mixed linear model estimated breed effects on mineral concentrations. No significant sire breed (P >= 0.43) or dam breed (P >= 0.20) effects were identified for mineral concentrations. Pearson correlation coefficients were calculated among mineral concentrations, carcass, and sensory traits. Zinc concentration was positively correlated (P <= 0.05) with total iron (r = 0.14), heme iron (r = 0.13), and magnesium (r = 0.19). Significant (P < 0.05) correlations were identified between non-heme or heme iron and most traits in this study. Magnesium concentration was correlated with all carcass and palatability traits. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Duan, Q.; Beitz, D. C.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Tait, R. G., Jr.; Schneider, M. J.; Beitz, D. C.; Reecy, J. M.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Tait, R. G., Jr.; Wheeler, T. L.; Shackelford, S. D.; Cundiff, L. V.] USDA, ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Reecy, JM (reprint author), Iowa State Univ, Dept Anim Sci, 2255 Kildee Hall, Ames, IA 50011 USA. EM qduan@email.unc.edu; jr.tait@ars.usda.gov; schneider.matt.john@gmail.com; dcbeitz@iastate.edu; tommy.wheeler@ars.usda.gov; steven.shackelford@ars.usda.gov; larry.cundiff@ars.usda.gov; jreecy@iastate.edu OI Tait, Jr., Richard/0000-0002-2379-7740 NR 33 TC 0 Z9 0 U1 1 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1740 EI 1873-4138 J9 MEAT SCI JI Meat Sci. PD AUG PY 2015 VL 106 BP 25 EP 30 DI 10.1016/j.meatsci.2015.03.020 PG 6 WC Food Science & Technology SC Food Science & Technology GA CI8VS UT WOS:000355050400005 PM 25866932 ER PT J AU Johnson, RB Kim, SK Watson, AM Barrows, FT Kroeger, EL Nicklason, PM Goetz, GW Place, AR AF Johnson, Ronald B. Kim, Shin-Kwon Watson, Aaron M. Barrows, Frederic T. Kroeger, Eric L. Nicklason, Peter M. Goetz, Giles W. Place, Allen R. TI Effects of dietary taurine supplementation on growth, feed efficiency, and nutrient composition of juvenile sablefish (Anoplopoma fimbria) fed plant based feeds SO AQUACULTURE LA English DT Article DE Taurine; Sablefish; Alternative feeds; 5 parameter saturated kinetics model ID FLOUNDER PARALICHTHYS-OLIVACEUS; YELLOWTAIL SERIOLA-QUINQUERADIATA; PERFORMANCE; FISH; REQUIREMENT; PRODUCTS; PROTEIN; COBIA; BODY AB Juvenile sablefish were fed a low taurine, basal feed with seven graded levels of supplemental taurine to determine taurine requirements for growth and feed efficiency. The basal feed was plant based, formulated primarily with soy and corn proteins with a minimal (9%) amount of fishmeal. The unsupplemented, basal feed contained 0.14% taurine. Experimental feeds were supplemented with 0, 0.25, 0.50, 1.0, 1.5, 3.0, and 6.0% taurine. Using the five parameter, saturated kinetic model (5 SKM), peak weight gain was predicted at 1.5% dietary taurine. Optimum weight gain, as defined as the region of the 5 SKM curve corresponding to at least 95% of peak, was predicted between 0.4% and 5.8% dietary taurine. Peak feed efficiency was predicted at 1.1% dietary taurine with optimum weight gain predicted between 0.4% and 4.2%. Whole body and muscle tissue protein and lipid content were not affected by taurine supplementation. Tissue taurine content increased asymptotically with increasing dietary taurine supplementation. Whole body tissue became saturated at 0.25 +/- 0.02% taurine, expressed on a wet weight basis. Muscle tissue became saturated at 0.34 +/- 0.02% taurine. Results fromthis study should increase the performance of alternative, plant based feeds formulated for sablefish and enable regulatory agencies better estimate the potential human exposure to taurine fromthe consumption of sablefish receiving these feeds. Published by Elsevier B.V. C1 [Johnson, Ronald B.; Kroeger, Eric L.; Nicklason, Peter M.; Goetz, Giles W.] Natl Marine Fisheries Serv, Environm & Fisheries Sci Div, Northwest Fisheries Sci Ctr, Seattle, WA 98112 USA. [Kim, Shin-Kwon] Natl Fisheries Res & Dev Inst, New Strategy Res Ctr, Busan 619705, South Korea. [Watson, Aaron M.; Place, Allen R.] Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21202 USA. [Watson, Aaron M.] Marine Resources Res Inst, South Carolina Dept Nat Resources, Charleston, SC 29412 USA. [Barrows, Frederic T.] ARS, USDA, Bozeman, MT 59715 USA. RP Johnson, RB (reprint author), Natl Marine Fisheries Serv, Environm & Fisheries Sci Div, Northwest Fisheries Sci Ctr, 2725 Montlake Blvd E, Seattle, WA 98112 USA. EM Ronald.B.Johnson@noaa.gov OI Johnson, Ronald/0000-0003-4362-123X FU US-Korea Joint Panel for Aquaculture [15-AQ-48]; Institute of Marine and Environmental Technology [15-148]; University of Maryland Center for Environmental Science [5015] FX The authors wish to acknowledge Thomas Scott, Lisa Armbruster, and Frank Sommers of the Aquaculture Program at NWFSC for care of experimental animals and fish culture system maintenance. The authors also thank Rick Goetz and the staff of the Marine Fish Program at NOAA Fisheries, Manchester, WA for the gift of juvenile sablefish for the study. The authors additionally thank Steven Rawles at the USDA Harry K. Dupree Stuttgart National Aquaculture Research Center, Stuttgart AR for his assistance with computer programing of the NLIN-procedure in SAS statistical software. The authors lastly thank Jason Frost from the U.S. Department of Agriculture for preparing feeds for the study at the U.S. Fish and Wildlife Service Bozeman Fish Technology Center, Bozeman, MT. This study was partially funded by the US-Korea Joint Panel for Aquaculture, grant #15-AQ-48. Additional funding was provided by contribution #15-148 of the Institute of Marine and Environmental Technology and contribution #5015 of the University of Maryland Center for Environmental Science. NR 41 TC 2 Z9 3 U1 4 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD AUG 1 PY 2015 VL 445 BP 79 EP 85 DI 10.1016/j.aquaculture.2015.03.030 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CI1FS UT WOS:000354489000009 ER PT J AU Locke, AM Ort, DR AF Locke, Anna M. Ort, Donald R. TI Diurnal depression in leaf hydraulic conductance at ambient and elevated [CO2] reveals anisohydric water management in field-grown soybean and possible involvement of aquaporins SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Leaf hydraulic conductance; Diurnal; Cavitation; Leaf water potential ID PLASMA-MEMBRANE AQUAPORINS; VESSEL-ASSOCIATED CELLS; LAURUS-NOBILIS; STOMATAL CONDUCTANCE; CIRCADIAN REGULATION; ENRICHMENT FACE; XYLEM EMBOLISM; CARBON-DIOXIDE; DOUGLAS-FIR; IN-VIVO AB Diurnal cycles of photosynthesis and water use in field-grown soybean (Glycine max) are tied to light intensity and vapor pressure deficit (VPD). At high mid-day VPD, transpiration rates can lead to a decline in leaf water potential (Psi(leaf)) if leaf hydraulic conductance (K-leaf) is insufficient to supply water to intercellular airspaces in pace with demand. K-leaf is determined by leaf xylem conductivity to water, as well as extra-xylem pathways that are likely mediated by aquaporin water transport proteins. When transpiration demand exceeds the maximum capacity of K-leaf to supply water, high tension in the water column can cause cavitation in xylem, and these emboli-blocked xylem vessels reduce water transport and thus lower K-leaf. Stomatal conductance typically remains high at mid-day for soybean, suggesting either a mid-day increase in K-leaf or that photosynthesis may be maintained at the cost of leaf water status, indicative of an anisohydric water management strategy in soybean. This study examined diurnal fluctuations in K-leaf and Psi(leaf), showing a mid-day depression in K-leaf in a pattern closely reflecting that of Psi(leaf), indicating that K-leaf depression is the result of cavitation in leaf xylem. The diurnal depression of K-leaf was not prevented by growth at elevated [CO2], which lowered stomatal conductance. Diurnal transcription patterns of aquaporin genes showed that a total of 34 genes belonging to 4 aquaporin families were expressed in soybean leaves, of which 22 were differentially expressed between at least two time points. These data suggest that mid-day K-leaf depression was driven primarily by cavitation at increasing xylem water tensions, but that aquaporins are also likely involved in diurnal regulation of soybean leaf water status. It is further concluded that because soybean photosynthesis is typically sustained at mid-day, K-leaf even at the depressed level was in excess of that needed to sustain a stomatal conductance sufficient to prevent depression of photosynthesis in soybean. (C) 2015 Elsevier B.V. All rights reserved. C1 [Locke, Anna M.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Locke, Anna M.; Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. RP Ort, DR (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM locke@ucr.edu; d-ort@igb.illinois.edu FU United States Department of Agriculture-Agricultural Research Service FX We thank Adrian Zimbleman, Rachel Yelin, Nathan Couch, Alex Hargus, Kannan Puthuval, Matthew Siebers, Sharon Gray, Courtney Leisner for assistance with field maintenance, measurements, and data analysis, and Andy VanLoocke for sharing temperature and humidity data. This work was funded by the United States Department of Agriculture-Agricultural Research Service. NR 66 TC 1 Z9 2 U1 10 U2 61 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD AUG PY 2015 VL 116 BP 39 EP 46 DI 10.1016/j.envexpbot.2015.03.006 PG 8 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA CI2NR UT WOS:000354584800005 ER PT J AU Porfiri, C Montoya, JC Koskinen, WC Azcarate, MP AF Porfiri, Carolina Montoya, Jorgelina C. Koskinen, William C. Azcarate, Mariela P. TI Adsorption and transport of imazapyr through intact soil columns taken from two soils under two tillage systems SO GEODERMA LA English DT Article DE Imazapyr; Adsorption; Leach; Tillage systems; Soil properties ID SOLUTE TRANSPORT; HERBICIDE IMAZAPYR; PREFERENTIAL FLOW; UNDISTURBED SOIL; SORPTION; IMAZETHAPYR; DESORPTION; IMAZAQUIN; IMIDAZOLINONE; MOBILITY AB The objective of this work was to study the sorption and transport of imazapyr (IMZ) in two soils with different physical-chemical properties, subject to long-term conventional tillage (CT) and no-tillage (NT) systems. Sorption was carried out using batch equilibrium method and high performance liquid chromatography analytical routines. Displacement experiments through intact soil cores were done using IMZ as reactive solute and bromide (Br-) as non-reactive solute. Equilibrium (CDEeq) and non-equilibrium (CDEnon-eq) transport models (CXTFIT 2.1) were employed to describe the breakthrough curves (BTCs). IMZ was only sorbed by NT soils with K-d values ranging from 0.22 to 1.1 L kg(-1). Clay content, pH and CEC values were the main factors affecting the sorption of IMZ. Miscible displacement studies showed that almost all the soil water was mobile and transport was related to physical equilibrium conditions. Some evidence of sorption non-equilibrium was seen in column experiments with IMZ; retardation factors were close to one, demonstrating the low sorption and the high potential to leach of this compound. (C) 2015 Elsevier B.V. All rights reserved. C1 [Porfiri, Carolina; Montoya, Jorgelina C.; Azcarate, Mariela P.] INTA, EEA Anguil, RA-6326 Anguil, La Pampa, Argentina. [Koskinen, William C.] Univ Minnesota, USDA ARS, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Porfiri, C (reprint author), INTA, EEA Anguil, Ruta Nacl 5,Km 580 CC 11, RA-6326 Anguil, La Pampa, Argentina. EM porfiri.carolina@inta.gob.ar FU INTA FX This study was conducted at the Agricultural Experimental Station of Anguil, La Pampa (Argentina) belonging to National Institute of Agricultural Technology (INTA). The financial support of this research by INTA is gratefully acknowledged. NR 51 TC 2 Z9 2 U1 6 U2 61 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD AUG PY 2015 VL 251 BP 1 EP 9 DI 10.1016/j.geoderma.2015.03.016 PG 9 WC Soil Science SC Agriculture GA CH0ZR UT WOS:000353751400001 ER PT J AU Hsi, DJ Ebel, ED Williams, MS Golden, NJ Schlosser, WD AF Hsi, David J. Ebel, Eric D. Williams, Michael S. Golden, Neal J. Schlosser, Wayne D. TI Comparing foodborne illness risks among meat commodities in the United States SO FOOD CONTROL LA English DT Article DE Risk assessment; Probability of illness; Risk management ID PUBLIC-HEALTH; OUTBREAK DATA; SOURCE ATTRIBUTION; PATHOGENS; FRAMEWORK; BURDEN AB Food-safety regulatory agencies are often tasked with oversight of a broad range of food commodities. For these agencies to regulate multiple commodities effectively, they need to develop policies and allocate resources that consider the varying magnitudes of the risk of illness that each of the commodities poses to the broad population of consumers. Process modeling is used in risk assessment to estimate the likelihood of illness by modeling contamination of raw foods, the microbial dynamics of pathogens between production and consumption, and dose-response relationships for the pathogen to estimate the risk and total number of illnesses for a specific commodity. Nevertheless, these models are usually unique to each commodity and constructed using different models and data sources, which can produce estimates that are difficult to compare. An alternative approach is presented that stems primarily from public health data. It uses simple methods to estimate various risk metrics simultaneously for multiple pathogens and commodities. This alternative approach is used to compare multiple risk metrics for beef, lamb, pork, and poultry for both Salmonella and Escherichia coli O157:H7. The implications of the different risk metrics are discussed with respect to current regulatory efforts in the United States. Published by Elsevier Ltd. C1 [Hsi, David J.] USDA, Food Safety & Inspect Serv, Off Field Operat, Ft Collins, CO 80526 USA. [Ebel, Eric D.; Williams, Michael S.; Golden, Neal J.; Schlosser, Wayne D.] USDA, Food Safety & Inspect Serv, Risk Assessment & Analyt Staff Off Publ Hlth Sci, Ft Collins, CO 80526 USA. RP Williams, MS (reprint author), USDA, Food Safety & Inspect Serv, Risk Assessment & Analyt Staff Off Publ Hlth Sci, 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM mike.williams@fsis.usda.gov NR 20 TC 1 Z9 1 U1 4 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD AUG PY 2015 VL 54 BP 353 EP 359 DI 10.1016/j.foodcont.2015.02.018 PG 7 WC Food Science & Technology SC Food Science & Technology GA CG1BO UT WOS:000353008200046 ER PT J AU Meisinger, JJ Palmer, RE Timlin, DJ AF Meisinger, John J. Palmer, Robert E. Timlin, Dennis J. TI Effects of tillage practices on drainage and nitrate leaching from winter wheat in the Northern Atlantic Coastal-Plain USA SO SOIL & TILLAGE RESEARCH LA English DT Article DE Lysimeter; Winter wheat; Drainage; Nitrate leaching; No-till; Plow-till ID SOIL HYDRAULIC-PROPERTIES; NO-TILLAGE; ORGANIC-CARBON; NITROGEN; CROP; DECOMPOSITION; MANAGEMENT; MOISTURE; COLUMNS; RESIDUE AB Management strategies for winter wheat (Triticum aestivum L., cultivar Pioneer "2548") in the Northern Atlantic Coastal-Plain region of the USA must balance agronomic production with practices to reduce nitrate-N (NO3-N) leaching, because much of this region lies within the Chesapeake Bay watershed. Winter wheat in this region is commonly grown using no-tillage (NT), but few studies have evaluated the effects of NT on NO3-N leaching for winter wheat. A four-year lysimeter study was conducted in Beltsville, Maryland by growing winter wheat in eight tension-drained undisturbed soil-column lysimeters (41 cm diameter by 100 cm deep) configured to exclude runoff, to compare the effects of NT vs. simulated plow-tillage (PT) and N fertilization practices on drainage volumes and NO3-N leaching. Additional data documented wheat total N uptake and bromide leaching. The temporal pattern of drainage showed that NT produced significant, but modestly higher, drainage volumes than PT during fall establishment (mid-Oct. to mid-Dec.), which increased the potential for NO3-N leaching, especially following high rainfalls. However, the greater risk of NO3-N leaching with NT during establishment decreased in the spring as tillage effects subsided and wheat water-use and N uptake became dominant. The increased fall leaching risk with NT can be managed by reducing fall-N applications, but both NT and PT can benefit from reduced NO3-N leaching resulting from spring split-N applications. The final NO3-N leaching losses depend on the interactions of precipitation timing and amount, fertilizer-N rate and timing, and initial soil water status. Additional winter-wheat NO3-N leaching research is needed to study to a wider range of soils, to assess high-residue vertical-tillage implements, and to evaluate precision application of spring fertilizer-N. Published by Elsevier B.V. C1 [Meisinger, John J.; Timlin, Dennis J.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Palmer, Robert E.] Univ Maryland, Dept Agron, College Pk, MD 20742 USA. RP Meisinger, JJ (reprint author), USDA ARS, Beltsville Agr Res Ctr, Beltsville Agr Res Ctr E, Room 6 Bldg 163F, Beltsville, MD 20705 USA. EM John.Meisinger@ars.usda.gov NR 42 TC 1 Z9 1 U1 12 U2 79 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD AUG PY 2015 VL 151 BP 18 EP 27 DI 10.1016/j.still.2015.02.007 PG 10 WC Soil Science SC Agriculture GA CG2GP UT WOS:000353093200003 ER PT J AU Zhang, BC Luo, YG Zhou, B Wang, Q Millner, PD AF Zhang, Boce Luo, Yaguang Zhou, Bin Wang, Qin Millner, Patricia D. TI A novel microfluidic mixer-based approach for determining inactivation kinetics of Escherichia coli O157:H7 in chlorine solutions SO FOOD MICROBIOLOGY LA English DT Article DE Microfluidic micromixer; Bacterial inactivation kinetics; Chlorine; Contact time ID LOW REYNOLDS-NUMBERS; CROSS-CONTAMINATION; PRODUCE WASH; WATER; DISINFECTION; LETTUCE; TIME; SALMONELLA; SURVIVAL; EFFICACY AB Determination of the minimum free chlorine concentration needed to prevent pathogen survival/cross-contamination during produce washing is essential for the development of science-based food safety regulations and practices. Although the trend of chlorine concentration-contact time on pathogen inactivation is generally understood, specific information on chlorine and the kinetics of pathogen inactivation at less than 1.00 s is urgently needed by the produce processing industry. However, conventional approaches to obtain this critical data have been unable to adequately measure very rapid responses. This paper reports our development, fabrication, and test of a novel microfluidic device, and its application to obtain the necessary data on pathogen inactivation by free chlorine in produce wash solution in times as short as 0.10 s. A novel microfluidic mixer with the capability to accurately determine the reaction time and control the chlorine concentration was designed with three inlets for bacterial, chlorine and dechlorinating solutions, and one outlet for effluent collection. The master mold was fabricated on a silicon wafer with microchannels via photopolymerization. Polydimethylsiloxane replicas with patterned microchannels were prototyped via soft lithography. The replicas were further assembled into the micromixer on glass via O-2 plasma treatment, and the inlets were connected to a syringe pump for solution delivery. To determine the kinetics of free chlorine on pathogen inactivation, chlorine solutions of varying concentrations were first pumped into the micromixer, together with the addition of bacterial suspension of Escherichia coli O157:H7 through a separate inlet. This was followed by injection of dechlorinating solution to stop the chlorine-pathogen reaction. The effluent was collected and the surviving bacteria cells were enumerated using a modified 'Most Probable Number' method. Free chlorine concentration was determined using a standard colorimetric method. The contact time was experimentally set by adjusting the solution flow rate, and was estimated by computational fluid dynamics modeling. Results showed that 1) pathogen inactivation was significantly affected by free chlorine concentration (P < 0.0001) and subsecond reaction time (P < 0.0001) and their interactions (P < 0.0001); and 2) the current industry practice of using 1.0 mg/L free chlorine will require more than 1.00 s total contact to achieve a 5-log(10) reduction in an E. coli O157:H7 population, whereas a 10.0 mg/L free chlorine solution will achieve 5-log(10) reduction in as little as 0.25 s. Information obtained from this study will provide critical insight on kinetics of bacterial inactivation for a broad range of sanitizers and produce wash operational conditions, thus facilitating the development and implementation of science-based food safety regulations and practices for improving food safety. Published by Elsevier Ltd. C1 [Zhang, Boce; Luo, Yaguang; Zhou, Bin; Millner, Patricia D.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Zhang, Boce; Zhou, Bin; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Luo, Yaguang] ARS, Food Qual Lab, USDA, Beltsville, MD 20705 USA. RP Luo, YG (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. EM yaguang.luo@ars.usda.gov FU USDA-NIFA Specialty Crop Research Initiative Grant [2010-51181-21230]; FabLab at the Maryland NanoCenter, University of Maryland, College Park, MD, USA FX This work is supported by USDA-NIFA Specialty Crop Research Initiative Grant Award No. 2010-51181-21230. We acknowledge the support of the FabLab at the Maryland NanoCenter, University of Maryland, College Park, MD, USA. NR 34 TC 2 Z9 2 U1 4 U2 62 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD AUG PY 2015 VL 49 BP 152 EP 160 DI 10.1016/j.fm.2015.01.013 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CE9ZS UT WOS:000352202900017 PM 25846925 ER PT J AU Carter, MQ Chapman, MH Gabler, F Brandl, MT AF Carter, Michelle Qiu Chapman, Mary H. Gabler, Franka Brandl, Maria T. TI Effect of sulfur dioxide fumigation on survival of foodborne pathogens on table grapes under standard storage temperature SO FOOD MICROBIOLOGY LA English DT Article DE Escherichia coli O157:H7; Listeria monocytogenes; Salmonella enterica; Sulfur dioxide; Fumigation; Table grapes ID ESCHERICHIA-COLI O157-H7; FRESH-CUT PIECES; LISTERIA-MONOCYTOGENES; SALMONELLA-ENTERICA; DISEASE OUTBREAKS; LEAF SURFACES; GRAY MOLD; LETTUCE; O157H7; VIRULENCE AB We examined the fate of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella enterica Thompson inoculated on freshly-harvested table grapes under standard cold storage with initial and weekly sulfur dioxide (SO2) fumigation. L. monocytogenes and S. enterica Thompson were much more sensitive to cold temperature than E. coli O157:H7. Furthermore, L. monocytogenes was highly susceptible to SO2. Initial fumigation with 100 or 200 ppm-hr was sufficient to eliminate this pathogen on grapes with low (104 cells/grape) and high (106 cells/grape) inocula, respectively. Initial fumigation with 300 ppm-hr reduced S. enterica Thompson population about 300- and 10-fold on grapes with low and high inocula, respectively. Initial fumigation with 300 ppm-hr reduced E. coli O157: H7 population to less than 10-fold, regardless of inoculum density. When grapes were inoculated with the high inoculum and fumigated on days 0 and 7 with 200 or 300 ppm-hr SO2, S. enterica Thompson and E. coli O157: H7 were completely inactivated between days 8 and 14 of cold storage. Standard cold storage combined with SO2 fumigation was effective in reducing and eliminating all three pathogens on table grapes, however, depending on the dose, two or three fumigations were needed for elimination of S. enterica Thompson and E. coli O157:H7. Published by Elsevier Ltd. C1 [Carter, Michelle Qiu; Chapman, Mary H.; Brandl, Maria T.] USDA ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Washington, DC 20250 USA. [Gabler, Franka] Calif Table Grape Commiss, Fresno, CA USA. RP Carter, MQ (reprint author), Western Reg Res Ctr, Produce Safety & Microbiol Unit, 800 Buchanan St, Albany, CA 94710 USA. EM michelle.carter@ars.usda.gov FU California Table Grape Commission (Trust Fund Cooperative Agreement) [58-5325-3-010-CA]; USDA-ARS CRIS project [5325-42000-046-00D] FX The authors wish to thank Jacqueline Louie for her technical support. This work was supported by the California Table Grape Commission (Trust Fund Cooperative Agreement No. 58-5325-3-010-CA Table Grape Commission) and USDA-ARS CRIS project 5325-42000-046-00D. NR 47 TC 3 Z9 3 U1 2 U2 33 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD AUG PY 2015 VL 49 BP 189 EP 196 DI 10.1016/j.fm.2015.02.002 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CE9ZS UT WOS:000352202900022 PM 25846930 ER PT J AU Sommers, C Rajkowski, KT Scullen, OJ Cassidy, J Fratamico, P Sheen, S AF Sommers, Christopher Rajkowski, Kathleen T. Scullen, O. Joseph Cassidy, Jennifer Fratamico, Pina Sheen, Shiowshuh TI Inactivation of Shiga Toxin-Producing Escherichia coli in lean ground beef by gamma irradiation SO FOOD MICROBIOLOGY LA English DT Article DE Gamma radiation; STEC; Ground beef; Virulence factors; Surrogates ID APPLE JUICE; O157-H7; RESISTANCE; PATHOGENS; RADIATION; HEAT AB In this study the radiation resistance of 40 Shiga Toxin-Producing Escherichia coli (STEC) isolates which contained various combinations of the shiga toxin 1 (stx1), shiga toxin 2 (stx2), intimin (eae), and hemolysin (ehx) genes were determined. The STEC were suspended in lean ground beef and irradiated at 4 degrees C. D-10 values, the radiation dose needed to reduce 1 log (90%) of a microorganism, ranged from 0.16 to 0.48 kGy, with a mean of 0.31 kGy for the 40 isolates. Isolates associated with illness outbreaks had a mean D-10 of 0.27 kGy, while non-outbreak isolates had a mean D-10 of 0.36 kGy (p < 0.05). The presence or absence of stx1, stx2, or both stx1 and 2 had no affect on D-10 (p > 0.05). The presence (0.30 kGy) or absence (0.35 kGy) of ehx had no affect on D-10 (p > 0.05). However, the mean D10 of isolates lacking eae (0.37 kGy) were significantly higher than those containing eae (0.27 kGy) (p < 0.05). There was no difference in D10 for isolates lacking eae regardless of whether or not they were associated with a foodborne illness outbreak (p > 0.05). It may be possible to use some of the STEC isolates which lacked eae, ehx, or both (D-10 > 0.30) as avirulent surrogates in food irradiation research. The data presented in this study provides risk assessors data for metagenomic analysis as well as food and radiation processors with valuable information to control of STEC in meat. Published by Elsevier Ltd. C1 [Sommers, Christopher; Rajkowski, Kathleen T.; Scullen, O. Joseph; Cassidy, Jennifer; Fratamico, Pina; Sheen, Shiowshuh] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Sommers, C (reprint author), USDA ARS, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM Christopher.sommers@ars.usda.gov NR 29 TC 3 Z9 3 U1 5 U2 33 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD AUG PY 2015 VL 49 BP 231 EP 234 DI 10.1016/j.fm.2015.02.013 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CE9ZS UT WOS:000352202900028 PM 25846936 ER PT J AU Chung, SY Mattison, CP Reed, S Wasserman, RL Desormeaux, WA AF Chung, Si-Yin Mattison, Christopher P. Reed, Shawndrika Wasserman, Richard L. Desormeaux, Wendy A. TI Treatment with oleic acid reduces IgE binding to peanut and cashew allergens SO FOOD CHEMISTRY LA English DT Article DE Oleic acid; Peanut allergen; Cashew allergen; Ana o 2; Sulfo-phospho-vanillin; IgE ID ALPHA-LACTALBUMIN; BETA-LACTOGLOBULIN; ORAL IMMUNOTHERAPY; SODIUM OLEATE; FOOD ALLERGY; TUMOR-CELLS; FATTY-ACID; OLIVE OIL; COMPLEXES; TEMPERATURE AB Oleic acid (OA) is known to bind and change the bioactivities of proteins, such as alpha-lactalbumin and beta-lactoglobulin in vitro. The objective of this study was to determine if OA binds to allergens from a peanut extract or cashew allergen and changes their allergenic properties. Peanut extract or cashew allergen (Ana o 2) was treated with or without 5 mM sodium oleate at 70 degrees C for 60 min (11) or under the same conditions with an additional overnight incubation at 37 degrees C (T2). After treatment, the samples were dialyzed and analyzed by SDS-PAGE and for OA content. IgE binding was evaluated by ELISA and western blot, using a pooled serum or plasma from individuals with peanut or cashew allergies. Results showed that OA at a concentration of 5 mM reduced IgE binding to the allergens. Peanut sample T2 exhibited a lower IgE binding and a higher OA content (protein-bound) than T1. Cashew allergen T2 also showed a reduction in IgE binding. We conclude that OA reduces the allergenic properties of peanut extract and cashew allergen by binding to the allergens. Our findings indicate that OA in the form of sodium oleate may be potentially useful as a coating to reduce the allergenic properties of peanut and cashew allergens. Published by Elsevier Ltd. C1 [Chung, Si-Yin; Mattison, Christopher P.; Reed, Shawndrika; Desormeaux, Wendy A.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Wasserman, Richard L.] Univ Texas Dallas, SW Med Ctr, Dept Pediat, Allergy Immunol Res Ctr North Texas, Dallas, TX 75390 USA. RP Chung, SY (reprint author), USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. EM siyin.chung@ars.usda.gov FU U.S. Government FX The author thanks Sophia Wojkowski for assisting in literature search. The study was funded by the U.S. Government. NR 36 TC 0 Z9 0 U1 7 U2 49 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD AUG 1 PY 2015 VL 180 BP 295 EP 300 DI 10.1016/j.foodchem.2015.02.056 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CE6QA UT WOS:000351961800038 PM 25766831 ER PT J AU Souza, MA Oliveira, JE Medeiros, ES Glenn, GM Mattoso, LHC AF Souza, Michelle A. Oliveira, Juliano E. Medeiros, Eliton S. Glenn, Gregory M. Mattoso, Luiz H. C. TI Controlled Release of Linalool Using Nanofibrous. Membranes of Poly(lactic acid) Obtained by Electrospinning and Solution Blow Spinning: A Comparative Study SO JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY LA English DT Article DE Electrospinning; Solution Blow Spinning; Nanofibers; Essential Oils; Controlled Release; Nanostructure ID ANTIMICROBIAL AGENTS; FILMS; FIBERS; NANOFIBERS; PLASTICIZERS; WETTABILITY; OIL; DIFFUSION; BUBBLE; AROMA AB The controlled-release of natural plant oils such as linalool is of interest in therapeutics, cosmetics, and antimicrobial and larvicidal products. The present study reports the release characteristics of linalool encapsulated at three concentrations (10, 15 and 20 wt.%) in poly(lactic acid) nanofibrous membranes produced by electrospinning and solution blow spinning (SBS) as well as the effect of linalool on fiber morphology and structural properties. PLA nanofibrous membranes were characterized by Scanning Electron Microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and contact angle measurements. The average diameters of the electrospun and solution blow spun nanofibers were similar, ranging from 176 to 240 nm. Linalool behaved as a plasticizer to PLA decreasing the glass transition temperature (T-g), melting point (T-m) and crystallization temperature (T-c) of PLA. Curves of the release of linalool at 35 degrees C were non-linear, showing a clear biphasic pattern consistent with one or more Fickian release components. The time required to release 50% of linalool (t(1/2)) decreased with increasing linalool concentration. The range in t(1/2) values for SBS nanofibers was higher (291-1645s) than the t(1/2) values for electrospun fibers (76-575s). C1 [Souza, Michelle A.; Mattoso, Luiz H. C.] Embrapa Instrumentacao CNPDIA, LNNA, BR-13560970 Sao Carlos, SP, Brazil. [Oliveira, Juliano E.; Medeiros, Eliton S.] Univ Fed Paraiba UFPB, Dept Engn Mat DEMAT, BR-58051900 Joao Pessoa, Paraiba, Brazil. [Glenn, Gregory M.] ARS, Bioprod Chem & Engn, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Mattoso, LHC (reprint author), Embrapa Instrumentacao CNPDIA, LNNA, Rua 15 Novembro,1452 Ctr, BR-13560970 Sao Carlos, SP, Brazil. RI Mattoso, Luiz H C/D-2794-2016 OI Mattoso, Luiz H C/0000-0001-7586-1014 FU National research council (CNPq) [163161/2011-9]; Sao Paulo State (FAPESP) [02010/19860-1]; Brazilian (CAPES) FX The authors are thankful to the National research council (CNPq, 163161/2011-9), Sao Paulo State (FAPESP, 02010/19860-1) and Brazilian (CAPES) funding agencies for the financial support to this research. NR 50 TC 7 Z9 7 U1 8 U2 147 PU AMER SCIENTIFIC PUBLISHERS PI VALENCIA PA 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA SN 1533-4880 EI 1533-4899 J9 J NANOSCI NANOTECHNO JI J. Nanosci. Nanotechnol. PD AUG PY 2015 VL 15 IS 8 BP 5628 EP 5636 DI 10.1166/jnn.2015.9692 PG 9 WC Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter SC Chemistry; Science & Technology - Other Topics; Materials Science; Physics GA CC3RW UT WOS:000350268100022 PM 26369129 ER PT J AU Bao, H Kommadath, A Liang, GX Sun, X Arantes, AS Tuggle, CK Bearson, SMD Plastow, GS Stothard, P Guan, LL AF Bao, Hua Kommadath, Arun Liang, Guanxiang Sun, Xu Arantes, Adriano S. Tuggle, Christopher K. Bearson, Shawn M. D. Plastow, Graham S. Stothard, Paul Guan, Le Luo TI Genome-wide whole blood microRNAome and transcriptome analyses reveal miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swine SO SCIENTIFIC REPORTS LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; INTRACELLULAR PATHOGENS; BACTERIAL-INFECTION; DIVERSE FUNCTIONS; IMMUNE-RESPONSES; GENE-EXPRESSION; DOWN-REGULATION; IRON TRANSPORT; INNATE; PROTEIN AB To understand the role of miRNAs in regulating genes involved in host response to bacterial infection and shedding of foodborne pathogens, a systematic profiling of miRNAs and mRNAs from the whole blood of pigs upon Salmonella challenge was performed. A total of 62 miRNAs were differentially expressed post infection (false discovery rate <0.1). An integrative analysis of both the differentially expressed miRNAs and mRNAs using sequence-based miRNA target prediction and negative correlation of miRNA-mRNA profiles helped identify miRNA-mRNA networks that may potentially regulate host response to Salmonella infection. From these networks, miR-214 and miR-331-3p were identified as new candidates potentially associated with Salmonella infection. An miRNA seed sequence analysis suggested that these miRNAs regulate several critical immune-related genes including SLC11A1, PIGE-108A11.3 and VAV2. We showed that challenged pigs had reduced miR-214 expression and increased miR-331-3p expression in the whole blood. Furthermore, the expression of the proposed targets of miR-214 (SLC11A1 and PIGE-108A11.3) increased while that of the proposed target of miR-331-3p (VAV2) decreased following challenge (expression changes confirmed by in vitro assays). Based on these observations, we propose potential roles for miR-214 and miR-331-3p in regulation of immune responses to Salmonella infection. C1 [Bao, Hua; Kommadath, Arun; Liang, Guanxiang; Sun, Xu; Arantes, Adriano S.; Plastow, Graham S.; Stothard, Paul; Guan, Le Luo] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. [Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Bearson, Shawn M. D.] USDA ARS, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, Ames, IA 50010 USA. RP Stothard, P (reprint author), Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. EM stothard@ualberta.ca; lguan@ualberta.ca FU Applied Livestock Genomics Program - Genome Alberta [ALGP13]; Alberta Livestock and Meat Agency; Large-Scale Applied Research Project; Application of Genomics to Improve Swine Health and Welfare; Genome Canada; Genome Alberta; Alberta Livestock and Meat Agency [2010R097S]; [USDA493 NIFA-2009-35205-05192] FX This work is supported by the Applied Livestock Genomics Program (ALGP13) funded by Genome Alberta and the Alberta Livestock and Meat Agency, and the Large-Scale Applied Research Project, Application of Genomics to Improve Swine Health and Welfare, funded by Genome Canada, Genome Alberta and the Alberta Livestock and Meat Agency. Blood samples were provided from Salmonella challenge studies conducted at USDA-ARS-NADC in a project funded by USDA493 NIFA-2009-35205-05192. Graham Plastow, Le Luo Guan, and Paul Stothard are grateful for the financial support of the Alberta Livestock and Meat Agency (project number 2010R097S). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 59 TC 3 Z9 3 U1 1 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 31 PY 2015 VL 5 AR 12620 DI 10.1038/srep12620 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN9LN UT WOS:000358770300001 PM 26227241 ER PT J AU Vega, FE Brown, SM Chen, H Shen, E Nair, MB Ceja-Navarro, JA Brodie, EL Infante, F Dowd, PF Pain, A AF Vega, Fernando E. Brown, Stuart M. Chen, Hao Shen, Eric Nair, Mridul B. Ceja-Navarro, Javier A. Brodie, Eoin L. Infante, Francisco Dowd, Patrick F. Pain, Arnab TI Draft genome of the most devastating insect pest of coffee worldwide: the coffee berry borer, Hypothenemus hampei SO SCIENTIFIC REPORTS LA English DT Article ID RNA-SEQ READS; GLUTATHIONE TRANSFERASES; TRIBOLIUM-CASTANEUM; GENE FAMILY; RESISTANCE; EVOLUTION; BEETLE; IDENTIFICATION; COLEOPTERA; EXPRESSION AB The coffee berry borer, Hypothenemus hampei, is the most economically important insect pest of coffee worldwide. We present an analysis of the draft genome of the coffee berry borer, the third genome for a Coleopteran species. The genome size is ca. 163 Mb with 19,222 predicted protein-coding genes. Analysis was focused on genes involved in primary digestion as well as gene families involved in detoxification of plant defense molecules and insecticides, such as carboxylesterases, cytochrome P450, gluthathione S-transferases, ATP-binding cassette transporters, and a gene that confers resistance to the insecticide dieldrin. A broad range of enzymes capable of degrading complex polysaccharides were identified. We also evaluated the pathogen defense system and found homologs to antimicrobial genes reported in the Drosophila genome. Ten cases of horizontal gene transfer were identified with evidence for expression, integration into the H. hampei genome, and phylogenetic evidence that the sequences are more closely related to bacterial rather than eukaryotic genes. The draft genome analysis broadly expands our knowledge on the biology of a devastating tropical insect pest and suggests new pest management strategies. C1 [Vega, Fernando E.] ARS, USDA, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Brown, Stuart M.; Chen, Hao; Shen, Eric] NYU, Sch Med, NYU Ctr Hlth Informat & Bioinformat, New York, NY 10016 USA. [Nair, Mridul B.; Pain, Arnab] King Abdullah Univ Sci & Technol, Computat Biosci Res Ctr, Pathogen Genom Lab, Thuwal Jeddah, Saudi Arabia. [Ceja-Navarro, Javier A.; Brodie, Eoin L.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Dept Ecol, Berkeley, CA 94720 USA. [Infante, Francisco] El Colegio Frontera ECOSUR, Tapachula 30700, Chiapas, Mexico. [Dowd, Patrick F.] ARS, USDA, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Vega, FE (reprint author), ARS, USDA, Sustainable Perennial Crops Lab, Bldg 001, Beltsville, MD 20705 USA. EM Fernando.Vega@ars.usda.gov RI Brodie, Eoin/A-7853-2008; Pain, Arnab/L-5766-2015; Ceja-Navarro, Javier/A-1731-2013; Brown, Stuart/A-9880-2016; OI Brodie, Eoin/0000-0002-8453-8435; Pain, Arnab/0000-0002-1755-2819; Ceja-Navarro, Javier/0000-0002-2954-3477; Brown, Stuart/0000-0002-0906-9907; Infante, Francisco/0000-0002-7419-7606; Vega, Fernando E./0000-0001-8103-5640 FU USDA-ARS [58-1245-3-309]; Center for Health Informatics and Bioinformatics at New York University [58-1245-3-309]; Lawrence Berkeley National Laboratory under U.S. Department of Energy [DE-AC02-05CH11231]; King Abdullah University of Science and Technology (KAUST); Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory under U.S. Department of Energy [DE-AC02-05CH1121231] FX Supported in part by Research Support Agreement 58-1245-3-309 between USDA-ARS and the Center for Health Informatics and Bioinformatics at New York University. Part of this work was performed at Lawrence Berkeley National Laboratory under U.S. Department of Energy Contract No. DE-AC02-05CH11231. The work was also supported by faculty baseline research funding (BRF) by King Abdullah University of Science and Technology (KAUST). J.A.C.-N. was supported in part by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory under U.S. Department of Energy Contract DE-AC02-05CH1121231. This work has used computing resources at the High Performance Computing Facility of the Center for Health Informatics and Bioinformatics at the NYU Langone Medical Center. NR 60 TC 6 Z9 6 U1 4 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 31 PY 2015 VL 5 AR 12525 DI 10.1038/srep12525 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN9MH UT WOS:000358772400001 PM 26228545 ER PT J AU Anderegg, WRL Schwalm, C Biondi, F Camarero, JJ Koch, G Litvak, M Ogle, K Shaw, JD Shevliakova, E Williams, AP Wolf, A Ziaco, E Pacala, S AF Anderegg, W. R. L. Schwalm, C. Biondi, F. Camarero, J. J. Koch, G. Litvak, M. Ogle, K. Shaw, J. D. Shevliakova, E. Williams, A. P. Wolf, A. Ziaco, E. Pacala, S. TI Pervasive drought legacies in forest ecosystems and their implications for carbon cycle models SO SCIENCE LA English DT Article ID AMAZON RAIN-FOREST; CLIMATE-CHANGE; RESILIENCE; EXTREMES; VARIABILITY; ALLOCATION; FEEDBACKS; SAVANNA; DIEBACK; CANOPY AB The impacts of climate extremes on terrestrial ecosystems are poorly understood but important for predicting carbon cycle feedbacks to climate change. Coupled climate-carbon cycle models typically assume that vegetation recovery from extreme drought is immediate and complete, which conflicts with the understanding of basic plant physiology. We examined the recovery of stem growth in trees after severe drought at 1338 forest sites across the globe, comprising 49,339 site-years, and compared the results with simulated recovery in climate-vegetation models. We found pervasive and substantial "legacy effects" of reduced growth and incomplete recovery for 1 to 4 years after severe drought. Legacy effects were most prevalent in dry ecosystems, among Pinaceae, and among species with low hydraulic safety margins. In contrast, limited or no legacy effects after drought were simulated by current climate-vegetation models. Our results highlight hysteresis in ecosystem-level carbon cycling and delayed recovery from climate extremes. C1 [Anderegg, W. R. L.; Wolf, A.; Ziaco, E.] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Anderegg, W. R. L.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA. [Anderegg, W. R. L.] Univ Arizona, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Schwalm, C.; Koch, G.] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Schwalm, C.] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86011 USA. [Biondi, F.; Ziaco, E.] Univ Nevada, DendroLab, Reno, NV 89557 USA. [Biondi, F.; Ziaco, E.] Univ Nevada, Grad Program Ecol Evolut & Conservat Biol, Reno, NV 89557 USA. [Camarero, J. J.] CSIC, Inst Pirenaico Ecol, Zaragoza 50192, Spain. [Litvak, M.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Ogle, K.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Shaw, J. D.] US Forest Serv, Rocky Mt Res Stn, Ogden, UT 84401 USA. [Shevliakova, E.] NOAA, Geophys Fluid Dynam Lab, Princeton, NJ 08540 USA. [Williams, A. P.] Columbia Univ, Lamont Doherty Earth Observ, Palisades, NY 10964 USA. RP Anderegg, WRL (reprint author), Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. EM anderegg@princeton.edu RI Biondi, Franco/G-2536-2010; Williams, Park/B-8214-2016; OI Biondi, Franco/0000-0003-0651-104X; Williams, Park/0000-0001-8176-8166; Camarero, J. Julio/0000-0003-2436-2922 FU NSF [DEB EF-1340270]; NOAA Climate and Global Change Postdoctoral Fellowship FX Funding for this research was provided by NSF (grant no. DEB EF-1340270). W.R.L.A. was supported in part by a NOAA Climate and Global Change Postdoctoral Fellowship, administered by the University Corporation for Atmospheric Research. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the opinions or policies of the funding agencies. All tree-ring data are available at www.ncdc.noaa.gov/data-access/paleoclimatology-data/datasets/tree-ring. We thank all data contributers at the International Tree-Ring Data Bank. All CMIP5 data are available at http://cmip-pcmdi.llnl.gov/cmip5/data_portal.html. We acknowledge the World Climate Research Programme's Working Group on Coupled Modelling, which is responsible for CMIP, and we thank the climate modeling groups (listed in the supplementary materials) for producing and making available their model output. For CMIP, the U.S. Department of Energy's Program for Climate Model Diagnosis and Intercomparison provides coordinating support and led the development of software infrastructure in partnership with the Global Organization for Earth System Science Portals. NR 38 TC 47 Z9 48 U1 34 U2 163 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JUL 31 PY 2015 VL 349 IS 6247 BP 528 EP 532 DI 10.1126/science.aab1833 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN8SP UT WOS:000358713300054 PM 26228147 ER PT J AU Ayars, JE Fulton, A Taylor, B AF Ayars, J. E. Fulton, A. Taylor, B. TI Subsurface drip irrigation in California-Here to stay? SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Subsurface drip irrigation; Processing tomato; Prune; Walnut; Almonds ID WATER; MICROIRRIGATION; QUALITY; SURFACE; CROPS; YIELD; CORN; SOIL AB Subsurface drip irrigation (SDI) has been used in California for over 30 years. Adoption occurred first in high value annual row crops. Over the years as drip irrigation materials, installation equipment, and irrigation scheduling tools have evolved, SDI has gained wider acceptance and is now being used in perennial crops as well on a limited basis. We discuss the early research on SDI in California and provide examples of the current commercial practices in both annual and perennial crops. These examples demonstrate how research preceded on-farm adoption and contributed to the implementation of SDI in California's production agriculture. SDI is being implemented throughout the world and these examples of implementation in production agriculture will be of interest in countries adopting the technology. Significant benefits are identified in terms of increased yield, improved crop quality, reduction in applied water and reduced agronomic costs for weed control, fertilization, and tillage. Improved water management is crucial for a sustainable future and SDI will be one tool that is available to improve water productivity. Published by Elsevier B.V. C1 [Ayars, J. E.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Fulton, A.] Univ Calif, Tehama Glenn Colusa & Shasta Counties, Cooperat Extens, Red Bluff, CA USA. [Taylor, B.] Brock Taylor Consulting, CPAG CPSS CCA, Clovis, CA 93619 USA. RP Ayars, JE (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM james.ayars@ars.usda.gov; aefulton@ucanr.edu; 1cpag@comcast.net NR 31 TC 10 Z9 13 U1 15 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JUL 31 PY 2015 VL 157 SI SI BP 39 EP 47 DI 10.1016/j.agwat.2015.01.001 PG 9 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CJ4ZU UT WOS:000355498000005 ER PT J AU Saseendran, SA Ahuja, LR Ma, LW Trout, TJ McMaster, GS Nielsen, DC Ham, JM Andales, AA Halvorson, AD Chavez, JL Fang, QXX AF Saseendran, S. A. Ahuja, Lajpat R. Ma, Liwang Trout, Thomas J. McMaster, Gregory S. Nielsen, David C. Ham, Jay M. Andales, Allan A. Halvorson, Ardel D. Chavez, Jose L. Fang, Quanxiao X. TI Developing and normalizing average corn crop water production functions across years and locations using a system model SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Cropping system model; Limited irrigation; Water management; Crop water production function ID CENTRAL GREAT-PLAINS; DEFICIT IRRIGATION; SOIL-WATER; NITROGEN-FERTILIZATION; WHEAT PRODUCTION; HYBRID MODEL; RZWQM; MANAGEMENT; ROTATION; PLANT AB Crop water production functions (CWPFs) are often expressed as crop yield vs. consumptive water use or irrigation water applied. CWPFs are helpful for optimizing management of limited water resources, but are site-specific and vary from year to year, especially when yield is expressed as a function of irrigation water applied. Designing limited irrigation practices requires deriving CWPFs from long-term field data to account for variation in precipitation and other climatic variables at a location. However, long-term field experimental data are seldom available. We developed location-specific (soil and climate) long-term averaged CWPFs for corn (Zea mays L.) using the Root Zone Water Quality Model (RZWQM2) and 20 years (1992-2011) of historical weather data from three counties of Colorado. Mean CWPFs as functions of crop evapotranspiration (ET), ET due to irrigation (ETa-d), irrigation (I), and plant water supply (PWS = effective rainfall + plant available water in the soil profile at planting + applied irrigation) were developed for three soil types at each location. Normalization of the developed CWPF across soils and climates was also developed. A Cobb-Douglas type response function was used to explain the mean yield responses to applied irrigations and extend the CWPFs for drip, sprinkler and surface irrigation methods, respectively, assuming irrigation application efficiencies of 95, 85 and 55%, respectively. The CWPFs developed for corn, and other crops, are being used in an optimizer program for decision support in limited irrigation water management in Colorado. (C) 2014 Elsevier B.V. All rights reserved. C1 [Saseendran, S. A.; Ahuja, Lajpat R.; Ma, Liwang; McMaster, Gregory S.] ARS, Agr Syst Res Unit, USDA, Ft Collins, CO 80526 USA. [Trout, Thomas J.] ARS, Water Management Res Unit, USDA, Ft Collins, CO 80526 USA. [Nielsen, David C.] ARS, Cent Great Plains Res Stn, USDA, Akron, CO 80720 USA. [Ham, Jay M.; Andales, Allan A.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Halvorson, Ardel D.] ARS, Soil Plant Nutr Res Unit, USDA, Ft Collins, CO 80526 USA. [Chavez, Jose L.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Fang, Quanxiao X.] Qingdao Agr Univ, Qingdao 266109, Peoples R China. RP Saseendran, SA (reprint author), ARS, Agr Syst Res Unit, USDA, Ft Collins, CO 80526 USA. EM Saseendran.Anapalli@ars.usda.gov RI Nielsen, David/A-8044-2009; OI Nielsen, David/0000-0002-8240-7183; Trout, Thomas/0000-0003-1896-9170 NR 43 TC 8 Z9 8 U1 3 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JUL 31 PY 2015 VL 157 SI SI BP 65 EP 77 DI 10.1016/j.agwat.2014.09.002 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CJ4ZU UT WOS:000355498000008 ER PT J AU Shanley, JB Sebestyen, SD McDonnell, JJ McGlynn, BL Dunne, T AF Shanley, James B. Sebestyen, Stephen D. McDonnell, Jeffrey J. McGlynn, Brian L. Dunne, Thomas TI Water's Way at Sleepers River watershed - revisiting flow generation in a post-glacial landscape, Vermont USA SO HYDROLOGICAL PROCESSES LA English DT Article DE runoff; watershed; isotopes; nutrients; streamflow ID RUNOFF PROCESSES; NEW-ZEALAND; STREAMFLOW GENERATION; BASEFLOW SEPARATION; ISOTOPE HYDROLOGY; SUBSURFACE-FLOW; DOUBLE PARADOX; OVERLAND-FLOW; STORM RUNOFF; CATCHMENT AB The Sleepers River Research Watershed (SRRW) in Vermont, USA, has been the site of active hydrologic research since 1959 and was the setting where Dunne and Black demonstrated the importance and controls of saturation-excess overland flow (SOF) on streamflow generation. Here, we review the early studies from the SRRW and show how they guided our conceptual approach to hydrologic research at the SRRW during the most recent 25years. In so doing, we chronicle a shift in the field from early studies that relied exclusively on hydrometric measurements to today's studies that include chemical and isotopic approaches to further elucidate streamflow generation mechanisms. Highlights of this evolution in hydrologic understanding include the following: (i) confirmation of the importance of SOF to streamflow generation, and at larger scales than first imagined; (ii) stored catchment water dominates stream response, except under unusual conditions such as deep frozen ground; (iii) hydrometric, chemical and isotopic approaches to hydrograph separation yield consistent and complementary results; (iv) nitrate and sulfate isotopic compositions specific to atmospheric inputs constrain new water contributions to streamflow; and (v) convergent areas, or hillslope hollows', contribute disproportionately to event hydrographs. We conclude by summarizing some remaining challenges that lead us to a vision for the future of research at the SRRW to address fundamental questions in the catchment sciences. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Shanley, James B.] US Geol Survey, Montpelier, VT 05602 USA. [Sebestyen, Stephen D.] US Forest Serv, USDA, Grand Rapids, MI USA. [McDonnell, Jeffrey J.] Univ Saskatchewan, Saskatoon, SK, Canada. [McDonnell, Jeffrey J.] Univ Aberdeen, Aberdeen, Scotland. [McGlynn, Brian L.] Duke Univ, Durham, NC USA. [Dunne, Thomas] Univ Calif Santa Barbara, Santa Barbara, CA 93106 USA. RP Shanley, JB (reprint author), US Geol Survey, Montpelier, VT 05602 USA. EM jshanley@usgs.gov RI McGlynn, Brian/A-2509-2008; Sebestyen, Stephen/D-1238-2013 OI McGlynn, Brian/0000-0001-5266-4894; Sebestyen, Stephen/0000-0002-6315-0108 NR 94 TC 7 Z9 7 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD JUL 30 PY 2015 VL 29 IS 16 SI SI BP 3447 EP 3459 DI 10.1002/hyp.10377 PG 13 WC Water Resources SC Water Resources GA CN5CH UT WOS:000358446700002 ER PT J AU Oh, MH Bender, KW Kim, SY Wu, X Lee, S Nou, IS Zielinski, RE Clouse, SD Huber, SC AF Oh, Man-Ho Bender, Kyle W. Kim, Sang Y. Wu, Xia Lee, Seulki Nou, Iii-Sup Zielinski, Raymond E. Clouse, Steven D. Huber, Steven C. TI Functional analysis of the BRI1 receptor kinase by Thr-for-Ser substitution in a regulatory autophosphorylation site SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE BRI1; kinase domain; directed mutagenesis; autophosphorylation ID TYROSINE PHOSPHORYLATION; BRASSINOSTEROID RECEPTOR; ARABIDOPSIS-THALIANA; ACTIVATION; GROWTH; DOMAIN; BAK1; TRANSPHOSPHORYLATION; SPECIFICITY; MECHANISMS AB BRI1 becomes highly phosphorylated in vivo upon perception of the ligand, brassinolide, as a result of autophosphorylation and transphosphorylation by its co-receptor kinase, BAK1. Important autophosphorylation sites include those involved in activation of kinase activity and those that are inhibitory, such as Ser-891. The inhibitory sites are autophosphorylated after kinase activation has been achieved and are postulated to contribute to deactivation of the kinase. The function of phosphosites is usually tested by substituting a non-phosphorylatable residue or an acidic residue that can act as a phosphomimetic. What has typically not been examined is substitution of a Thr for a Ser phosphosite (or vice versa) but given that Thr and Ser are not equivalent amino acids this type of substitution may represent a new approach to engineer regulatory phosphorylation. In the present study with BRI1, we substituted Thr at the Ser-891 phosphosite to generate the S891T directed mutant. The recombinant Flag-BRI1 (S891T) cytoplasmic domain protein (the S891T protein) was catalytically active and phosphorylation occurred at the engineered Thr-891 site. However, the S891T recombinant protein autophosphorylated more slowly than the wild-type protein during expression in E. coil. As a result, activation of peptide kinase activity (measured in vitro) was delayed as was transphosphorylation of bacterial proteins in situ. Stable transgenic expression of BRI1 (5891T)-Flag in Arabidopsis bri1-5 plants did not fully rescue the brassinosteroid (BR) phenotype indicating that BR signaling was constrained. Our working model is that restricted signaling in the 5891T plants occurs as a result of the reduced rate of activation of the mutant BRI1 kinase by autophosphorylation. These results provide the platform for future studies to critically test this new model in vivo and establish Ser-Thr substitutions at phosphosites as an interesting approach to consider with other protein kinases. C1 [Oh, Man-Ho; Lee, Seulki] Chungnam Natl Univ, Coll Biol Sci & Biotechnol, Dept Biol Sci, Plant Dev Genet, Taejon 305764, South Korea. [Oh, Man-Ho; Bender, Kyle W.; Kim, Sang Y.; Zielinski, Raymond E.; Huber, Steven C.] Univ Illinois, Dept Plant Biol, Prot Biochem, Urbana, IL 61801 USA. [Kim, Sang Y.; Huber, Steven C.] ARS, USDA, Urbana, IL USA. [Wu, Xia] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Nou, Iii-Sup] Sunchon Natl Univ, Dept Hort, Sunchon, South Korea. [Clouse, Steven D.] NC State Univ, Dept Hort Sci, Raleigh, NC USA. RP Oh, MH (reprint author), Chungnam Natl Univ, 99 Daekak Ro, Taejon 305764, South Korea. EM manhooh@cnu.ac.kr; schuber1@illinois.edu OI Bender, Kyle/0000-0002-1805-8097 FU National Science Foundation [IOS-1022177, MCB-1021363]; US Department of Agriculture (USDA)-Agricultural Research Service (ARS); Chungnam National University; Golden Seed Project (Center for Horticultural Seed Development) [213003-04-3-SB110]; Ministry of Agriculture, Food and Rural Affairs (MAFRA); Ministry of Oceans and Fisheries(MOF); Rural Development Administration (RDA); Korea Forest Service (KFS); National Research Foundation of Korea (NRF) - Ministry of Education [2014R1A1A401006751] FX This work was supported in part by the National Science Foundation (IOS-1022177 and MCB-1021363) and the US Department of Agriculture (USDA)-Agricultural Research Service (ARS) and this research was supported in part by research fund of Chungnam National University and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2014R1A1A401006751) and Golden Seed Project (Center for Horticultural Seed Development, No. 213003-04-3-SB110), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries(MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS). NR 35 TC 1 Z9 1 U1 1 U2 15 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUL 30 PY 2015 VL 6 AR 562 DI 10.3389/fpls.2015.00562 PG 11 WC Plant Sciences SC Plant Sciences GA CN8WR UT WOS:000358726900001 PM 26284086 ER PT J AU Jenkins, MC Fetterer, R Miska, K Tuo, W Kwok, O Dubey, JP AF Jenkins, M. C. Fetterer, R. Miska, K. Tuo, W. Kwok, O. Dubey, J. P. TI Characterization of the Eimeria maxima sporozoite surface protein IMP1 SO VETERINARY PARASITOLOGY LA English DT Article DE Eimeria; Immunoprotective protein; Gene expression ID PROTECTIVE IMMUNITY; CHICKENS; TENELLA; COCCIDIOSIS AB The purpose of this study was to characterize Eimeria maxima immune-mapped protein 1 (IMP1) that is hypothesized to play a role in eliciting protective immunity against E. maxima infection in chickens. RT-PCR analysis of RNA from unsporulated and sporulating E. maxima oocysts revealed highest transcription levels at 6-12 h of sporulation with a considerable downregulation thereafter. Alignment of IMP1 coding sequence from Houghton, Weybridge, and APU-1 strains of E. maxima revealed single nucleotide polymorphisms that in some instances led to amino acid changes in the encoded protein sequence. The E. maxima (APU-1) IMP1 cDNA sequence was cloned and expressed in 2 different polyHis Escherichia coli expression vectors. Regardless of expression vector, recombinant E. maxima IMP1 (rEmaxIMP1) was fairly unstable in non-denaturing buffer, which is consistent with stability analysis of the primary amino acid sequence. Antisera specific for rEmaxIMP1 identified a single 72 kDa protein or a 61 kDa protein by non-reducing or reducing SDS-PAGE/immunoblotting. Immunofluorescence staining with anti-rEmaxIMP1, revealed intense surface staining of E. maxima sporozoites, with negligible staining of merozoite stages. Immuno-histochemical staining of E. maxima-infected chicken intestinal tissue revealed staining of E. maxima developmental stages in the lamnia propia and crypts at both 24 and 48 h post-infection, and negligible staining thereafter. The expression of IMP1 during early stages of in vivo development and its location on the sporozoite surface may explain in part the immunoprotective effect of this protein against E. maxima infection. Published by Elsevier B.V. C1 [Jenkins, M. C.; Fetterer, R.; Tuo, W.; Kwok, O.; Dubey, J. P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Miska, K.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Jenkins, MC (reprint author), ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM mark.jenkins@ars.usda.gov FU USDA-ARS CRIS project "Development and Control of Intervention Strategies for Avian Coccidiosis" [1265-31320-0076-00D] FX The authors acknowledge the technical assistance of Carolyn Parker in the study. This project was solely funded by the USDA-ARS CRIS project "Development and Control of Intervention Strategies for Avian Coccidiosis"- Project No. 1265-31320-0076-00D. NR 21 TC 2 Z9 3 U1 0 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 30 PY 2015 VL 211 IS 3-4 BP 146 EP 152 DI 10.1016/j.vetpar.2015.05.009 PG 7 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CN0GY UT WOS:000358093300005 PM 26012860 ER PT J AU Li, RW Wu, ST Li, CJ Li, WZ Schroeder, SG AF Li, Robert W. Wu, Sitao Li, Cong-Jun Li, Weizhong Schroeder, Steven G. TI Splice variants and regulatory networks associated with host resistance to the intestinal worm Cooperia oncophora in cattle SO VETERINARY PARASITOLOGY LA English DT Article DE Cattle; Ruminants; RNA-seq; Splice variants; Gene fusion; Network; Resistance; Parasite ID SMOOTH-MUSCLE CONTRACTILITY; GASTROINTESTINAL NEMATODES; NIPPOSTRONGYLUS-BRASILIENSIS; IMMUNE-SYSTEM; INFECTION; EXPRESSION; GENES; CELLS; IL-13; STAT6 AB To elucidate the molecular mechanism of host resistance, we characterized the jejunal transcriptome of Angus cattle selected for parasite resistance for over 20 years in response to infection caused by the intestinal worm Cooperia oncophora. The transcript abundance of 56 genes, such as that of mucin 12 (MUC12) and intestinal alkaline phosphatase (ALPI), was significantly higher in resistant cattle. Novel splicing variants, exon skipping events, and gene fusion events, were also detected. An algorithm for the reconstruction of accurate cellular networks (ARACNE) was used to infer de novo regulatory molecular networks in the interactome between the parasite and host. Under a combined cutoff of an error tolerance (epsilon = 0.10) and a stringent P-value threshold of mutual information (1.0 x 10(-5)), a total of 229,100 direct interactions controlled by 20,288 hub genes were identified. Among these hub genes, 7651 genes had >= 100 direct neighbors while the top 9778 hub genes controlled more than 50% of total direct interactions. Three lysozyme genes (LYZ1, LYZ2, and LYZ3), which are co-located in bovine chromosome 5 in tandem and are strongly upregulated in resistant cattle, shared a common regulatory network of 55 genes. These ancient antimicrobials were likely involved in regulating host-parasite interactions by affecting host gut microbiome. Notably, ALPI, known as a gut mucosal defense factor, controlled a molecular network consisting 410 genes, including 14 transcription factors (TF) and 10 genes that were significantly regulated in resistant cattle. Several large regulatory networks were controlled by TF, such as STAT6, SREBF1, and ELF4. Gene ontology (GO) processes significantly enriched in the regulatory network controlled by STAT6 included lipid metabolism. Our findings provide insights into the immune regulation of host-parasite interactions and the molecular mechanisms of host resistance in cattle. Published by Elsevier B.V. C1 [Li, Robert W.; Li, Cong-Jun; Schroeder, Steven G.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Wu, Sitao; Li, Weizhong] Univ Calif San Diego, Ctr Res Biol Syst, San Diego, CA 92093 USA. [Li, Weizhong] J Craig Venter Inst, La Jolla, CA 92037 USA. RP Li, RW (reprint author), ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM Robert.Li@ars.usda.gov RI Li, Weizhong/A-9735-2008 OI Schroeder, Steven/0000-0001-9103-5150; Li, Weizhong/0000-0003-1804-9403 FU USDA National Institute of Food and Agriculture [2013-67015-22957] FX We would like to thank Dr. Louis C. Gasbarre and Deborah Hebert for their technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer. SW and WL were partially supported by the USDA National Institute of Food and Agriculture under Award No. 2013-67015-22957 to WL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 0 Z9 0 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 30 PY 2015 VL 211 IS 3-4 BP 241 EP 250 DI 10.1016/j.vetpar.2015.05.010 PG 10 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CN0GY UT WOS:000358093300018 PM 26025321 ER PT J AU Yang, YR Ying, YQ Verma, SK Cassinelli, ABM Kwok, OCH Liang, HD Pradhan, AK Zhu, XQ Su, C Dubey, JP AF Yang, Yurong Ying, Yuqing Verma, S. K. Cassinelli, A. B. M. Kwok, O. C. H. Liang, Hongde Pradhan, A. K. Zhu, X. Q. Su, C. Dubey, J. P. TI Isolation and genetic characterization of viable Toxoplasma gondii from tissues and feces of cats from the central region of China SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Feces; Cat (Fells catus); Isolation; Genotype; China ID STRAY CATS; PREVALENCE; GENOTYPE; PROVINCE; ANIMALS; DOGS AB Cats are important in the epidemiology of toxoplasmosis because they are the only definitive hosts that excrete environmentally resistant Toxoplasma gondii oocysts. Little is known of feline toxoplasmosis in China and most of the literature is in Chinese. Here we summarized all published reports on feline toxoplasmosis in English and report first identification of oocyst excretion by naturally infected cats in China. Unfrozen tissues of 42 cats and feces of 360 cats from China were bioassayed in mice for isolation of T. gondii. Antibodies to T. gondii were found in 21 of 42 (50%) of cats by the modified agglutination test (cut-off 1:25). Viable T. gondii was isolated from tissues of eight of 21 seropositive but not from 21 seronegative (<1:25) cats. Viable T. gondii was isolated from feces of one cat. DNA derived from cell cultured tachyzoites of all nine T. gondii isolates was characterized by PCR RFLP at 10 loci (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). Four genotypes were found; the genotypes of tissue isolates were ToxoDB #9 in six, ToxoDB #2 in one, and ToxoDB #17 in one. The fecal isolate was ToxoDB #1. To our knowledge, the present study is the first isolation of T. gondii from cat feces from China. (C) 2015 Published by Elsevier B.V. C1 [Yang, Yurong; Liang, Hongde] Henan Agr Univ, Coll Anim Sci & Vet Med, Lab Vet Pathol, Zhengzhou 450002, Peoples R China. [Ying, Yuqing; Pradhan, A. K.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Verma, S. K.; Cassinelli, A. B. M.; Kwok, O. C. H.; Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Pradhan, A. K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Zhu, X. Q.] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China. [Su, C.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov NR 41 TC 5 Z9 6 U1 2 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 30 PY 2015 VL 211 IS 3-4 BP 283 EP 288 DI 10.1016/j.vetpar.2015.05.006 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CN0GY UT WOS:000358093300023 PM 26033402 ER PT J AU Ramirez-Carvajal, L Rodriguez, LL AF Ramirez-Carvajal, Lisbeth Rodriguez, Luis L. TI Virus-resistant pigs might help to stem next outbreak SO ELIFE LA English DT Editorial Material ID MOUTH-DISEASE; INTERFERENCE; RNA C1 [Ramirez-Carvajal, Lisbeth; Rodriguez, Luis L.] ARS, Plum Isl Anim Dis Ctr, USDA, Orient Point, NY 11957 USA. [Ramirez-Carvajal, Lisbeth] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN USA. RP Ramirez-Carvajal, L (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Orient Point, NY 11957 USA. EM luis.rodriguez@ars.usda.gov NR 9 TC 1 Z9 1 U1 1 U2 3 PU ELIFE SCIENCES PUBLICATIONS LTD PI CAMBRIDGE PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND SN 2050-084X J9 ELIFE JI eLife PD JUL 29 PY 2015 VL 4 AR e09790 DI 10.7554/eLife.09790 PG 2 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CO1OK UT WOS:000358924700001 PM 26222499 ER PT J AU Harry-O'kuru, RE Tisserat, B Gordon, SH Gravet, A AF Harry-O'kuru, Rogers E. Tisserat, Brent Gordon, Sherald H. Gravet, Alan TI Osage Orange (Maclura pomifera L.) Seed Oil Poly(alpha-hydroxydibutylamine) Triglycerides: Synthesis and Characterization SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Osage orange; seed oil; epoxidation; amination; poly(alpha-hydroxydibutylamine) triglyceride AB Milled Osage orange seeds (Maclura pomifera (Raf.) Schneid) were Soxhlet extracted:with hexane, and portions of the extract were treated with activated carbon before solvent removal. The crude oil was winterized and degummed by centrifugation at low temperature. Decantation of the centrifugate gave an admixture of the triglycerides and free fatty acids. The free fatty acid content of the oil was removed when portions of the admixture were diluted with hexane and shaken With cold aqueous ammonium hydroxide (0.1 M) solution. The desiccant dried organic phase was concentrated under reduced pressure to give: the cleaned Osage orange triglyceride after solvent removal by rotary evaporation at 67 degrees C. Epoxidation of the-resulting, cleaned triglyceride was effected by reaction with in situ generated peroxy performic acid in H2O2. The oxirane rings' of the derivatized oil were then opened using N,N-dibutylamine catalyzed by anhydrous ZnCl2 to afford the poly(alpha-hydtotydibutylamine) triglyceride. The purpose of this work was to derivatize and thereby stabilize this highly unsaturated tree oil for its eventual use in lubrication applications: C1 [Harry-O'kuru, Rogers E.] USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, Peoria, IL 61604 USA. [Tisserat, Brent] USDA ARS, Natl Ctr Agr Utilizat Res, Funct Food Res Unit, Peoria, IL 61604 USA. [Gordon, Sherald H.] USDA ARS, Natl Ctr Agr Utilizat Res, Plant Polymer Res Unit, Peoria, IL 61604 USA. [Gravet, Alan] Hedge Apple Biotech, Bloomington, IL 61704 USA. RP Harry-O'kuru, RE (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Rogers.HarryOkuru@ars.usda.gov NR 20 TC 0 Z9 0 U1 3 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 29 PY 2015 VL 63 IS 29 BP 6588 EP 6595 DI 10.1021/acs.jafc.5b01625 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CO1EJ UT WOS:000358896200011 PM 26189408 ER PT J AU Saski, CA Bhattacharjee, R Scheffler, BE Asiedu, R AF Saski, Christopher A. Bhattacharjee, Ranjana Scheffler, Brian E. Asiedu, Robert TI Genomic Resources for Water Yam (Dioscorea alata L.): Analyses of EST-Sequences, De Novo Sequencing and GBS Libraries SO PLOS ONE LA English DT Article ID MILLET SETARIA-ITALICA; RICE ORYZA-SATIVA; MICROSATELLITE MARKERS; DNA; GENERATION; RESISTANCE; ALIGNMENT; FEATURES AB The reducing cost and rapid progress in next-generation sequencing techniques coupled with high performance computational approaches have resulted in large-scale discovery of advanced genomic resources in several model and non-model plant species. Yam (Dioscorea spp.) is a major food and cash crop in many countries but research efforts have been limited to understand the genetics and generate genomic information for the crop. The availability of a large number of genomic resources including genome-wide molecular markers will accelerate the breeding efforts and application of genomic selection in yams. In the present study, several methods including expressed sequence tags (EST)-sequencing, de novo sequencing, and genotyping-by-sequencing (GBS) profiles on two yam (Dioscorea alata L.) genotypes (TDa 95/00328 and TDa 95-310) was performed to generate genomic resources for use in its improvement programs. This includes a comprehensive set of EST-SSRs, genomic SSRs, whole genome SNPs, and reduced representation SNPs. A total of 1,152 EST-SSRs were developed from >40,000 EST-sequences generated from the two genotypes. A set of 388 EST-SSRs were validated as polymorphic showing a polymorphism rate of 34% when tested on two diverse parents targeted for anthracnose disease. In addition, approximately 40X de novo whole genome sequence coverage was generated for each of the two genotypes, and a total of 18,584 and 15,952 genomic SSRs were identified for TDa 95/00328 and TDa 95-310, respectively. A custom made pipeline resulted in the selection of 573 genomic SSRs common across the two genotypes, of which only eight failed, 478 being polymorphic and 62 monomorphic indicating a polymorphic rate of 83.5%. Additionally, 288,505 high quality SNPs were also identified between these two genotypes. Genotyping by sequencing reads on these two genotypes also revealed 36,790 overlapping SNP positions that are distributed throughout the genome. Our efforts in using different approaches in generating genomic resources provides a non-biased glimpse into the publicly available EST-sequences, yam genome, and GBS profiles with affirmation that the genomic complexity can be methodically unraveled and constitute a critical foundation for future studies in linkage mapping, germplasm analysis, and predictive breeding. C1 [Saski, Christopher A.] Clemson Univ, Genom & Computat Biol Lab, Inst Translat Genom, Clemson, SC USA. [Bhattacharjee, Ranjana; Asiedu, Robert] Int Inst Trop Agr, Biosci Ctr, Ibadan, Nigeria. [Scheffler, Brian E.] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. RP Bhattacharjee, R (reprint author), Int Inst Trop Agr, Biosci Ctr, PMB 5320, Ibadan, Nigeria. EM r.bhattacharjee@cgiar.org OI Scheffler, Brian/0000-0003-1968-8952 FU USAID-Linkage grant through the International Institute of Tropical Agriculture FX RB thanks USAID-Linkage grant awarded through the International Institute of Tropical Agriculture for sponsoring the research. The funding agency had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 2 Z9 2 U1 3 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 29 PY 2015 VL 10 IS 7 AR e0134031 DI 10.1371/journal.pone.0134031 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CO0JM UT WOS:000358836800098 PM 26222616 ER PT J AU Vellichirammal, NN Wang, HC Eyun, SI Moriyama, EN Coates, BS Miller, NJ Siegfried, BD AF Vellichirammal, Neetha Nanoth Wang, Haichuan Eyun, Seong-il Moriyama, Etsuko N. Coates, Brad S. Miller, Nicholas J. Siegfried, Blair D. TI Transcriptional analysis of susceptible and resistant European corn borer strains and their response to Cry1F protoxin SO BMC GENOMICS LA English DT Article DE European corn borer; Ostrinia nubilalis; Insect resistance; Cry1F resistance; Bt-toxin; Transcriptomics; Cry1F response; RNA-Seq ID BRUSH-BORDER MEMBRANE; HELIOTHIS-VIRESCENS LEPIDOPTERA; OSTRINIA-NUBILALIS LEPIDOPTERA; BACILLUS-THURINGIENSIS TOXINS; RNA-SEQ DATA; MANDUCA-SEXTA; INSECT RESISTANCE; GENE-EXPRESSION; DELTA-ENDOTOXINS; CROSS-RESISTANCE AB Background: Despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from Bacillus thuringiensis (Bt), little is known about the mechanism of resistance to these toxins. The purpose of this study is to identify genes associated with the mechanism of Cry1F toxin resistance in European corn borer (Ostrinia nubilalis Hubner). For this, we compared the global transcriptomic response of laboratory selected resistant and susceptible O. nubilalis strain to Cry1F toxin. We further identified constitutive transcriptional differences between the two strains. Results: An O. nubilalis midgut transcriptome of 36,125 transcripts was assembled de novo from 106 million Illumina HiSeq and Roche 454 reads and used as a reference for estimation of differential gene expression analysis. Evaluation of gene expression profiles of midgut tissues from the Cry1F susceptible and resistant strains after toxin exposure identified a suite of genes that responded to the toxin in the susceptible strain (n = 1,654), but almost 20-fold fewer in the resistant strain (n = 84). A total of 5,455 midgut transcripts showed significant constitutive expression differences between Cry1F susceptible and resistant strains. Transcripts coding for previously identified Cry toxin receptors, cadherin and alkaline phosphatase and proteases were also differentially expressed in the midgut of the susceptible and resistant strains. Conclusions: Our current study provides a valuable resource for further molecular characterization of Bt resistance and insect response to Cry1F toxin in O. nubilalis and other pest species. C1 [Vellichirammal, Neetha Nanoth; Wang, Haichuan; Miller, Nicholas J.; Siegfried, Blair D.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Eyun, Seong-il] Univ Nebraska, Ctr Biotechnol, Lincoln, NE 68583 USA. [Moriyama, Etsuko N.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68583 USA. [Moriyama, Etsuko N.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68583 USA. [Coates, Brad S.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. RP Vellichirammal, NN (reprint author), Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. EM neetha@unl.edu; bsiegfried1@unl.edu OI Eyun, Seong-il/0000-0003-4687-1066 FU Dow AgroSciences; USDA NIFA Biotechnology Risk Assessment Grant Program [2010-33522-21673] FX This research was supported by Dow AgroSciences and the USDA NIFA Biotechnology Risk Assessment Grant Program (2010-33522-21673). The authors thank Terence A. Spencer for O. nubilalis colony maintenance and for his assistance in conducting toxicity assays and Nathan Palmer for helpful discussions during transcriptome assembly construction. This work was completed utilizing the Holland Computing Center of the University of Nebraska. NR 63 TC 1 Z9 2 U1 3 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 29 PY 2015 VL 16 AR 558 DI 10.1186/s12864-015-1751-6 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CN6SP UT WOS:000358564800004 ER PT J AU Troche, C Eicher, SD Applegate, TJ AF Troche, Catalina Eicher, Susan D. Applegate, Todd J. TI The influence of dietary zinc source and coccidial vaccine exposure on intracellular zinc homeostasis and immune status in broiler chickens SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Zinc transporters; Coccidia; Broilers; Zinc sources ID EIMERIA-ACERVULINA INFECTION; METALLOTHIONEIN GENE-EXPRESSION; COPPER INTERRELATIONSHIP; LYMPHOID-TISSUES; MAXIMA INFECTION; ABSORPTION; CELLS; LIVER; METABOLISM; ACTIVATION AB Coccidia are protozoal parasites which compromise mucosal integrity of the intestine, potentiating poultry morbidity. The host's Zn status influences the course of infection. Therefore, two experiments were designed to determine how supplemental Zn regimens impacted jejunal and caecal immune status and Zn transporter expression. Coccivac (R)-B was administered weekly at ten times the recommended dose as a mild coccidial challenge (10CV). Zn was provided through a basal diet, supplemental zinc sulfate (ZnSO4), or a supplemental 1:1 blend of ZnSO4 and Availa (R)-Zn (Blend). Mucosal jejunum (Expt 1) and caecal tonsils (Expt 2) were evaluated for intracellular Zn concentrations and phagocytic capacity. Messenger expression of Zn transporters ZnT5, ZnT7, Zip9 and Zip13 were investigated to determine Zn trafficking. With 10CV, phagocytic capacity was decreased in jejunal cells by 2 %. In the caecal tonsils, however, phagocytic capacity increased with challenge, with the magnitude of increase being more pronounced with higher dietary Zn (10CV x Zn interaction; P = 0.04). Intracellular Zn within caecal tonsils was found significantly reduced with 10CV (27 %, P = 0.0001). 10CV also resulted in an overall increase in the ratio of Zip:ZnT transporters. With the exception of Zip13 transporter expression, dietary Zn source had little impact on any of the measured cellular parameters. Thus, intestinal mucosal tissues had reductions in intracellular free Zn during coccidial challenge, which was coupled with an upregulation of measured Zip transporters. This suggests that under coccidial challenge, intestinal cells attempt to compensate for the drop in intracellular Zn. C1 [Troche, Catalina; Applegate, Todd J.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Eicher, Susan D.] ARS, Livestock Behav Res Unit, USDA, W Lafayette, IN USA. RP Applegate, TJ (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. EM applegt@purdue.edu OI Applegate, Todd/0000-0002-2406-5058 FU Zinpro Corporation (Eden Prarie, Minnesota, USA); Zinpro Corporation, Eden Prairie, MN, USA FX The authors would like to thank Zhengyu Jiang and Liting Xu for their assistance with tissue processing and sample preparation during the flow cytometry experiments. We also thank Kolapo Ajuwon for the generous use of his lab facilities and expertise with PCR analysis. The authors thank Zinpro Corporation (Eden Prarie, Minnesota, USA) for partial funding for the present research.; Partial funding for the present research reported herein was provided by the Zinpro Corporation, Eden Prairie, MN, USA as an unconditional research gift. Zinpro Corporation had no role in the design, analysis or writing of this article. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. United States Department of Agriculture is an equal opportunity employer. NR 70 TC 1 Z9 1 U1 4 U2 28 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD JUL 28 PY 2015 VL 114 IS 2 BP 202 EP 212 DI 10.1017/S0007114515001592 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CO3TZ UT WOS:000359084200005 PM 26079373 ER PT J AU Moore, GG Mack, BM Beltz, SB AF Moore, Geromy G. Mack, Brian M. Beltz, Shannon B. TI Genomic sequence of the aflatoxigenic filamentous fungus Aspergillus nomius SO BMC GENOMICS LA English DT Article DE Aspergillus nomius; Genome sequence; Gene ontology; Phylogenomics; Mating type locus ID SECTION FLAVI; GENE CLUSTERS; MATING-TYPE; ANNOTATION; IDENTIFICATION; PREDICTION; FUMIGATUS; ALIGNMENT; ORYZAE; MODEL AB Background: Aspergillus nomius is an opportunistic pathogen and one of the three most important producers of aflatoxins in section Flavi. This fungus has been reported to contaminate agricultural commodities, but it has also been sampled in non-agricultural areas so the host range is not well known. Having a similar mycotoxin profile as A. parasiticus, isolates of A. nomius are capable of secreting B- and G- aflatoxins. Results: In this study we discovered that the A. nomius type strain (NRRL 13137) has a genome size of approximately 36 Mb which is comparable to other Aspergilli whose genomes have been sequenced. Its genome encompasses 11,918 predicted genes, 72 % of which were assigned GO terms using BLAST2GO. More than 1,200 of those predicted genes were identified as unique to A. nomius, and the most significantly enriched GO category among the unique genes was oxidoreducatase activity. Phylogenomic inference shows NRRL 13137 as ancestral to the other aflatoxigenic species examined from section Flavi. This strain contains a single mating-type idiomorph designated as MAT1-1. Conclusions: This study provides a preliminary analysis of the A. nomius genome. Given the recently discovered potential for A. nomius to undergo sexual recombination, and based on our findings, this genome sequence provides an additional evolutionary reference point for studying the genetics and biology of aflatoxin production. C1 [Moore, Geromy G.; Mack, Brian M.; Beltz, Shannon B.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Moore, GG (reprint author), USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. EM geromy.moore@ars.usda.gov NR 42 TC 3 Z9 3 U1 1 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 28 PY 2015 VL 16 AR 551 DI 10.1186/s12864-015-1719-6 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CN5PD UT WOS:000358480400003 PM 26216546 ER PT J AU Topuz, M Nemli, Y Fatima, T Mattoo, AK AF Topuz, Muhamet Nemli, Yildiz Fatima, Tahira Mattoo, Autar K. TI Seed dormancy is modulated in recently evolved chlorsulfuron-resistant Turkish biotypes of wild mustard (Sinapis arvensis) SO FRONTIERS IN CHEMISTRY LA English DT Article DE Sinapis arvensis; ALS inhibitor; herbicide resistance; point mutations; seed dormancy; germination; gibberellins ID KOCHIA KOCHIA-SCOPARIA; SYNTHASE-INHIBITING HERBICIDES; SULFONYLUREA-RESISTANT; ECOLOGICAL FITNESS; GERMINATION; GENE; ARABIDOPSIS; POPULATION; MUTATIONS; EMERGENCE AB Biotypes of the broad-leaved wild mustard (Sinapis arvensis L.) found in wheat fields of Aegean and Marmara region of Turkey were characterized and shown to have developed resistance to sulfonylurea (chlorsulfuron), an inhibitor of acetolactate synthase (ALS). DNA sequence analysis of the ALS genes from two such resistant ("R") biotypes, KNF-R1 and KNF-R2, revealed point mutations, CCT (Pro 197) to TCT (Ser 197) in KNF-R1 and CCT (Pro 197) to ACT (Thr 197) in KNF-R2; these substitutions are consistent with the presence of chlorsulfuron-insensitive ALS enzyme activity in the "R" S. arvensis biotypes. An additional phenotype of chlorsulfuron resistance in the Turkish S. arvensis "R" biotypes was revealed in the form of an altered seed dormancy behavior over 4-48 months of dry storage (after-ripening) compared to the susceptible ("S") biotypes. Seeds of the "S" biotypes dry stored for 4 months had a higher initial germination, which sharply decreased with storage time, while the seeds of the "R" biotypes had lower germination after 4-months storage, rising sharply and peaking thereafter by 24 months' of dry storage. The "R" biotype seeds continued to maintain a higher germination percentage even after 48 months of after-ripening. The seed weight of "R" and "S" biotypes after-ripened for 4 months was similar but those after-ripened for 48 months differed, "R" seeds were significantly heavier than those of the "S" seeds. Differential seed germinability between "S" and "R" biotypes was found not a case of differential viability, temperature regimen or non-response to pro-germination hormone GA3. These studies are of relevance to ecological fitness of herbicide-resistant biotypes in terms of seed viability and germination. C1 [Topuz, Muhamet; Fatima, Tahira; Mattoo, Autar K.] ARS, Sustainable Agr Syst Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Bldg 001, Beltsville, MD 20705 USA. [Topuz, Muhamet; Nemli, Yildiz] Ege Univ, Dept Plant Protect, Plant Protect Res Stn, Izmir, Turkey. RP Mattoo, AK (reprint author), ARS, Sustainable Agr Syst Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Bldg 001, Beltsville, MD 20705 USA. EM autar.mattoo@ars.usda.gov NR 49 TC 0 Z9 0 U1 1 U2 1 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND EI 2296-2646 J9 FRONT CHEM JI Front. Chem. PD JUL 24 PY 2015 VL 3 AR 46 DI 10.3389/fchem.2015.00046 PG 11 WC Chemistry, Multidisciplinary SC Chemistry GA DI2XV UT WOS:000373363400001 PM 26258120 ER PT J AU Graebner, RC Wise, M Cuesta-Marcos, A Geniza, M Blake, T Blake, VC Butler, J Chao, SM Hole, DJ Horsley, R Jaiswal, P Obert, D Smith, KP Ullrich, S Hayes, PM AF Graebner, Ryan C. Wise, Mitchell Cuesta-Marcos, Alfonso Geniza, Matthew Blake, Tom Blake, Victoria C. Butler, Joshua Chao, Shiaomen Hole, David J. Horsley, Rich Jaiswal, Pankaj Obert, Don Smith, Kevin P. Ullrich, Steven Hayes, Patrick M. TI Quantitative Trait Loci Associated with the Tocochromanol (Vitamin E) Pathway in Barley SO PLOS ONE LA English DT Article ID GENOME-WIDE ASSOCIATION; ALPHA-TOCOPHEROL; GAMMA-TOCOPHEROL; BREEDING GERMPLASM; BETA-GLUCAN; LINKAGE MAP; BIOSYNTHESIS; HUMANS; TRIAL; SUPPLEMENTATION AB The Genome-Wide Association Studies approach was used to detect Quantitative Trait Loci associated with tocochromanol concentrations using a panel of 1,466 barley accessions. All major tocochromanol types- alpha-, beta-, delta-,gamma-tocopherol and tocotrienol-were assayed. We found 13 single nucleotide polymorphisms associated with the concentration of one or more of these tocochromanol forms in barley, seven of which were within 2 cM of sequences homologous to cloned genes associated with tocochromanol production in barley and/or other plants. These associations confirmed a prior report based on bi-parental QTL mapping. This knowledge will aid future efforts to better understand the role of toco-chromanols in barley, with specific reference to abiotic stress resistance. It will also be useful in developing barley varieties with higher tocochromanol concentrations, although at current recommended daily consumption amounts, barley would not be an effective sole source of vitamin E. However, it could be an important contributor in the context of whole grains in a balanced diet. C1 [Graebner, Ryan C.; Cuesta-Marcos, Alfonso; Hayes, Patrick M.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Wise, Mitchell] USDA ARS, Cereal Crops Res, Madison, WI USA. [Geniza, Matthew; Jaiswal, Pankaj] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Blake, Tom] Montana State Univ, Plant Sci & Plant Pathol Dept, Bozeman, MT 59717 USA. [Blake, Victoria C.] USDA ARS, Crop Improvement & Genet Res, Albany, CA USA. [Butler, Joshua] Busch Agr Resources Inc, Ft Collins, CO USA. [Chao, Shiaomen] USDA ARS, Cereal Crops Res Unit, Fargo, ND USA. [Hole, David J.] Utah State Univ, Plants Soils & Climate Dept, Logan, UT 84322 USA. [Horsley, Rich] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Obert, Don] Limagrain Cereal Seeds, Lafayette, IN USA. [Smith, Kevin P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. [Ullrich, Steven] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Hayes, PM (reprint author), Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. EM patrick.m.hayes@oregonstate.edu RI Jaiswal, Pankaj/H-7599-2016 OI Jaiswal, Pankaj/0000-0002-1005-8383 FU USDA-NIFA TCAP Project [2011-68002-30029]; USDA-NIFA Collaborative Project [2013-31100-06041]; Busch Agricultural Resources, Inc; Limagrain Cereal Seeds FX This project was supported by USDA-NIFA TCAP Project No. 2011-68002-30029, and USDA-NIFA Collaborative Project No. 2013-31100-06041. Busch Agricultural Resources, Inc and Limagrain Cereal Seeds provided support in the form of salaries for authors JB & DO, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 0 Z9 0 U1 2 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 24 PY 2015 VL 10 IS 7 AR e0133767 DI 10.1371/journal.pone.0133767 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN7NT UT WOS:000358622000135 PM 26208213 ER PT J AU Yang, CY Powell, CA Duan, YP Shatters, R Zhang, MQ AF Yang, Chuanyu Powell, Charles A. Duan, Yongping Shatters, Robert Zhang, Muqing TI Antimicrobial Nanoemulsion Formulation with Improved Penetration of Foliar Spray through Citrus Leaf Cuticles to Control Citrus Huanglongbing SO PLOS ONE LA English DT Article ID CANDIDATUS LIBERIBACTER-ASIATICUS; GOLDEN DELICIOUS APPLES; SAO-PAULO-STATE; DELIVERY-SYSTEMS; GREENING DISEASE; PLANT CUTICLES; SURFACTANTS; PERMEABILITY; SINENSIS; LEAVES AB Huanglongbing (HLB) is the most serious disease affecting the citrus industry worldwide to date. The causal agent, Candidatus Liberibacter asiaticus (Las), resides in citrus phloem, which makes it difficult to effectively treat with chemical compounds. In this study, a transcuticular nanoemulsion formulation was developed to enhance the permeation of an effective antimicrobial compound (ampicillin; Amp) against HLB disease through the citrus cuticle into the phloem via a foliar spray. The results demonstrated that efficiency of cuticle isolation using an enzymatic method (pectinase and cellulase) was dependent on the citrus cultivar and Las-infection, and it was more difficult to isolate cuticles from valencia orange (Citrus sinensis) and HLB-symptomatic leaves. Of eight adjuvants tested, Brij 35 provided the greatest increase in permeability of the HLB-affected cuticle with a 3.33-fold enhancement of cuticular permeability over water control. An in vitro assay using Bacillus subtilis showed that nanoemulsion formulations containing Amp (droplets size = 5.26 +/- 0.04 nm and 94 +/- 1.48 nm) coupled with Brij 35 resulted in greater inhibitory zone diameters (5.75 mm and 6.66 mm) compared to those of Brij 35 (4.34 mm) and Amp solution (2.83 mm) alone. Furthermore, the nanoemulsion formulations eliminated Las bacteria in HLB-affected citrus in planta more efficiently than controls. Our study shows that a water in oil (W/O) nanoemulsion formulation may provide a useful model for the effective delivery of chemical compounds into citrus phloem via a foliar spray for controlling citrus HLB. C1 [Yang, Chuanyu; Zhang, Muqing] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou, Peoples R China. [Yang, Chuanyu; Powell, Charles A.; Zhang, Muqing] Univ Florida, Indian River Res & Educ Ctr, IFAS, Ft Pierce, FL USA. [Duan, Yongping; Shatters, Robert] USDA ARS, Hort Res Lab, Ft Pierce, FL USA. [Zhang, Muqing] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agri B, Nanning 530004, Peoples R China. RP Zhang, MQ (reprint author), Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou, Peoples R China. EM mqzhang@ufl.edu FU Citrus Research and Development Foundation [584, 617]; Guangxi Natural Science Foundation [2013GXNSFCB1902]; Guangxi international Cooperation Project [1347004-7] FX Financial support was provided by Citrus Research and Development Foundation (project#584, #617 and their enhancement), Guangxi Natural Science Foundation (project 2013GXNSFCB1902) and Guangxi international Cooperation Project (project 1347004-7). NR 52 TC 6 Z9 6 U1 7 U2 32 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 24 PY 2015 VL 10 IS 7 AR e0133826 DI 10.1371/journal.pone.0133826 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN7NT UT WOS:000358622000151 PM 26207823 ER PT J AU Ma, C Wang, H Macnish, AJ Estrada-Melo, AC Lin, J Chang, YH Reid, MS Jiang, CZ AF Ma, Chao Wang, Hong Macnish, Andrew J. Estrada-Melo, Alejandro C. Lin, Jing Chang, Youhong Reid, Michael S. Jiang, Cai-Zhong TI Transcriptomic analysis reveals numerous diverse protein kinases and transcription factors involved in desiccation tolerance in the resurrection plant Myrothamnus flabellifolia SO HORTICULTURE RESEARCH LA English DT Article ID RESPONSIVE GENE-EXPRESSION; COLD STRESS RESPONSES; CRATEROSTIGMA-PLANTAGINEUM; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; ABIOTIC STRESS; SPOROBOLUS-STAPFIANUS; GALLOYLQUINIC ACID; DROUGHT TOLERANCE; OSMOTIC-STRESS AB The woody resurrection plant Myrothamnus flabellifolia has remarkable tolerance to desiccation. Pyro-sequencing technology permitted us to analyze the transcriptome of M. flabellifolia during both dehydration and rehydration. We identified a total of 8287 and 8542 differentially transcribed genes during dehydration and rehydration treatments respectively. Approximately 295 transcriptionfactors (TFs) and 484 protein kinases (PKs) were up-or down-regulated in response to desiccation stress. Among these, the transcript levels of 53 TFs and 91 PKs increased rapidly and peaked early during dehydration. These regulators transduce signal cascades of molecular pathways, including the up-regulation of ABA-dependent and independent drought stress pathways and the activation of protective mechanisms for coping with oxidative damage. Antioxidant systems are up-regulated, and the photosynthetic system is modified to reduce ROS generation. Secondary metabolism may participate in the desiccation tolerance of M. flabellifolia as indicated by increases in transcript abundance of genes involved in isopentenyl diphosphate biosynthesis. Up-regulation of genes encoding late embryogenesis abundant proteins and sucrose phosphate synthase is also associated with increased tolerance to desiccation. During rehydration, the transcriptome is also enriched in transcripts of genes encoding TFs and PKs, as well as genes involved in photosynthesis, and protein synthesis. The data reported here contribute comprehensive insights into the molecular mechanisms of desiccation tolerance in M. flabellifolia. C1 [Ma, Chao; Macnish, Andrew J.; Estrada-Melo, Alejandro C.; Reid, Michael S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Wang, Hong; Lin, Jing; Chang, Youhong] Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China. [Jiang, Cai-Zhong] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. RP Jiang, CZ (reprint author), ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. EM cjiang@ucdavis.edu NR 80 TC 5 Z9 6 U1 3 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD JUL 22 PY 2015 VL 2 AR 15034 DI 10.1038/hortres.2015.34 PG 12 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA CQ6EY UT WOS:000360698400001 PM 26504577 ER PT J AU Jackson, MA Appell, M Blackburn, JK AF Jackson, Michael A. Appell, Michael Blackburn, Judith K. TI Hydrodeoxygenation of Fructose to 2,5-Dimethyltetrahydrofuran Using a Sulfur Poisoned Pt/C Catalyst SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article ID D-MANNITOL; HYDROGENATION; CONVERSION; DEHYDRATION; CELLULOSE; WATER; HYDROGENOLYSIS; RUTHENIUM; EFFICIENT; MOLECULE AB Fructose has been hydrodeoxygenated to 2,5-dimethyltetrahydrofuran using a sulfided Pt/C catalyst. The reaction was carried out in a stirred reactor at 10.3 MPa H-2 and 175 degrees C which allowed a 10% fructose solution to be converted in 2 h. The selectivity was greatly enhanced by using ethanol as solvent with 95% ethanol giving 50% DMTHF versus 9% in water. The only intermediate found along the reaction pathway was 2,5-hexanedione. This is presumed to be hydrogenated to 2,5-hexanediol which then ring closes to DMTHF. Molecular simulation at the B3LYP/6-311++G(2df,2p) level was used to propose a reaction pathway from fructose to 2,5-hexanedione and then to explain the preference for the cis isomer of DMTHF. C1 [Jackson, Michael A.; Blackburn, Judith K.] ARS, Renewable Prod Technol Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Appell, Michael] ARS, Bacterial Foodborne Pathogens & Mycol Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Jackson, MA (reprint author), ARS, Renewable Prod Technol Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.jackson@ars.usda.gov NR 36 TC 3 Z9 3 U1 7 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD JUL 22 PY 2015 VL 54 IS 28 BP 7059 EP 7066 DI 10.1021/acsiecr.5b00766 PG 8 WC Engineering, Chemical SC Engineering GA CN6PA UT WOS:000358555500006 ER PT J AU Harry-O'kuru, RE Biresaw, G Murray, RE AF Harry-O'kuru, Rogers E. Biresaw, Girma Murray, Rex E. TI Polyamine Triglycerides: Synthesis and Study of Their Potential in Lubrication, Neutralization, and Sequestration SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE milkweed oil; polyhydroxy oil; oxidgtion; polyketone; polyamine triglyceride ID MILKWEED OIL; POLYHYDROXY TRIGLYCERIDES; DIETARY POLYAMINES; CANCER-THERAPY; DERIVATIVES; RETROSPECT; PROSPECT; HEALTH; FOODS; ACID AB Renewable resources have evoked a new awakening in both scientific and industrial circles in the past decade. Vegetable oil one category of renewables that is amenable as a'source of new industrial products Because the source feedstock, seeds, are environmentally friendly) the derivatized products from these at the end of their lifetime could also be benign when designed appropriately. Bioethanol and biodiesel are examples of biobased industrial products currently in the market place and have become resources for uplifting the rural economy. Biolubricants also tare playing a more prominent role because they have -become closely competitive-with petroleum based lubricants: These products are renewable because the crops from which the feedstuff for the biofuels and biolubricants are produced are grown annually in contrast to nonrenewable mineral sources. Added to their renewability is the inherent biodegradability of their end use products after their useful lifetime. In a recent study of the lubricity characteristics of peracylated polyhydroxy milkweed oil, the derivatives Were found to exhibit good oxidative stability as well as excellent antiwear properties. To further explore an expansion in the properties of such materials in lubrication and other applications, in this study the polyhydroxy (OH) moieties of derivatized milkweed triglycerides were replaced with -NHR groupings in the oil. In this process novel polyketo triglyceride intermediates leading to polyamine derivatives of the vegetable oil have been synthesized The polyamine triglyceride markedly improved the stability of the parent oil to oxidative stress. It has also attenuated the extreme viscosity of the starting polyhydroxy oil to a more useful product that could be amenable for use as a lubricating agent, for example, hydraulic fluid. Both the polyketone and polyimine intermediates of the polyamine have chelating properties, The intermediates and the polyamine-were characterized spectroseopically) tribologically, and theologically for their intrinsic properties. C1 [Harry-O'kuru, Rogers E.; Biresaw, Girma; Murray, Rex E.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Harry-O'kuru, RE (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Rogers.HarryOkuru@ars.usda.gov NR 26 TC 0 Z9 0 U1 1 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 22 PY 2015 VL 63 IS 28 BP 6422 EP 6429 DI 10.1021/acs.jafc.5b00598 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CN6PB UT WOS:000358555600017 PM 26154265 ER PT J AU Price, NPJ Hartman, TM Vermillion, KE AF Price, Neil P. J. Hartman, Trina M. Vermillion, Karl E. TI Nickel-Catalyzed Proton-Deuterium Exchange (HDX) Procedures for Glycosidic Linkage Analysis of Complex Carbohydrates SO ANALYTICAL CHEMISTRY LA English DT Article ID PER-O-METHYLATION; RANEY-NICKEL; SODIUM-BOROHYDRIDE; METHYLSULFINYL CARBANION; OXIDATIVE-DEGRADATION; SELECTIVE DEUTERATION; AROMATIC-COMPOUNDS; MASS-SPECTROMETRY; ISOTOPE-EXCHANGE; H/D EXCHANGE AB The structural analysis of complex carbohydrates typically requires the assignment of three parameters: monosaccharide composition, the position of glycosidic linkages between monosaccharides, and the potion and nature of noncarbohydrate substituents. The glycosidic linkage positions are often determined by permethylation analysis, but this can be complicated by high viscosity or poor solubility, resulting in under-methylation. This is a,drawback because an under-methylated position may be misinterpreted as the erroneous site of a linkage or substituent Here, we describe an alternative approach to linkage analysis that makes use of a nonreversible deuterium exchange of C-H protons on the carbohydrate backbone. The exchange reaction is conducted in deuterated water catalyzed by Raney nickel, and results in the selective exchange of CH protons adjacent to free hydroxyl groups. Hence, the position of the residual C-H protons is indicative of the position of glycosidic linkages or other substituents and can be readily assigned by heteronuclear single quantum coherence-nuclear magnetic resonance (HSQC-NMR) or, following suitable derivatization, by gas chromatography-mass spectroscopy (GC/MS) analysis. Moreover, because the only changes to the parent sugar are proton/deuterium exchanges, the composition and linkage analysis can be determined in a single step. C1 [Price, Neil P. J.; Hartman, Trina M.] USDA ARS NCAUR, Renewable Prod Technol, USDA, Peoria, IL 61604 USA. [Vermillion, Karl E.] USDA ARS NCAUR, Funct Foods Res Units, USDA, Peoria, IL 61604 USA. RP Price, NPJ (reprint author), USDA ARS NCAUR, Renewable Prod Technol, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM neil.price@ars.usda.gov NR 48 TC 0 Z9 0 U1 2 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD JUL 21 PY 2015 VL 87 IS 14 BP 7282 EP 7290 DI 10.1021/acs.analchem.5b01505 PG 9 WC Chemistry, Analytical SC Chemistry GA CN6PE UT WOS:000358555900041 PM 26075577 ER PT J AU Bahder, BW Bollinger, ML Sudarshana, MR Zalom, FG AF Bahder, B. W. Bollinger, M. L. Sudarshana, M. R. Zalom, F. G. TI Preparation of Mealybugs (Hemiptera: Pseudococcidae) for Genetic Characterization and Morphological Examination SO JOURNAL OF INSECT SCIENCE LA English DT Article DE mealybug; taxonomy; identification; slide-mount; DNA extraction ID SEQUENCES; CLASSIFICATION AB Mealybugs (Hemiptera: Pseudococcidae) are economically significant agricultural pests on many different crops. Because of their small size and lack of easily visible characters for identification, determination of their taxonomic status is difficult and requires technical competency to prepare a slide-mounted specimen. The standard mounting technique does not allow for analysis of the genome of the specimen. Conversely, preparatory techniques for genetic analysis of mealybugs cause either loss of the entire individual or physical damage that can make morphology-based identification difficult. This study describes a simple protocol that does not impact physical integrity of the specimen for fixation and microscopic examination yet enables simultaneous DNA extraction for DNA-based identification of four mealybug species. All species prepared yielded high quality slide mounts, identified as Planococcus citri Risso, Pseudococcus viburni Signoret, Rhizoecus kondonis Kuwana, or Rhizoecus californicus Ferris. DNA extracted in this manner had higher purity and yield in the final eluate than in samples extracted using standard methods. DNA extracted was successfully amplified by polymerase chain reaction using primers for the cytochrome oxidase I gene and subsequently sequenced for all specimens. This protocol is likely to be applicable to other Hemiptera taxa that are preserved by slide mounting, allowing for both the preparation of a high-quality voucher specimen for morphological identification and simultaneous analysis of DNA for the same specimen. The methods used are technically less challenging than current standard procedures. C1 [Bahder, B. W.; Bollinger, M. L.; Zalom, F. G.] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA. [Sudarshana, M. R.] Univ Calif Davis, Dept Plant Pathol, USDA ARS, Davis, CA 95616 USA. RP Bahder, BW (reprint author), Univ Calif Davis, Dept Entomol & Nematol, 1 Shields Ave, Davis, CA 95616 USA. EM bwbahder@ucdavis.edu FU United States Department of Agriculture - Agricultural Research Service Current Research Information System [2032-22000-015-00D, 2023-22000-015-29S]; Fruit Tree, Nut Tree and Grapevine Improvement Advisory Board of California Department of Food and Agriculture FX Funding for this research was provided by the United States Department of Agriculture - Agricultural Research Service Current Research Information System project number 2032-22000-015-00D and 2023-22000-015-29S as well as the Fruit Tree, Nut Tree and Grapevine Improvement Advisory Board of the California Department of Food and Agriculture. The authors thank Trent Lawler, Ashley Li, Meredith Castillo, and Marion Eng for technical assistance in the laboratory and Dr. Sudeep Bag for assistance with sequence data. NR 20 TC 0 Z9 0 U1 2 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUL 21 PY 2015 VL 15 AR 104 DI 10.1093/jisesa/iev086 PG 7 WC Entomology SC Entomology GA CN7LQ UT WOS:000358616400002 ER PT J AU Agga, GE Arthur, TM Durso, LM Harhay, DM Schmidt, JW AF Agga, Getahun E. Arthur, Terrance M. Durso, Lisa M. Harhay, Dayna M. Schmidt, John W. TI Antimicrobial-Resistant Bacterial Populations and Antimicrobial Resistance Genes Obtained from Environments Impacted by Livestock and Municipal Waste SO PLOS ONE LA English DT Article ID ESCHERICHIA-COLI O157-H7; POLYMERASE-CHAIN-REACTION; WATER-TREATMENT PLANTS; ANTIBIOTIC-RESISTANCE; SALMONELLA-ENTERICA; FEEDLOT CATTLE; MULTIDRUG-RESISTANT; UNITED-STATES; FECAL SAMPLES; TYPHIMURIUM AB This study compared the populations of antimicrobial-resistant bacteria and the repertoire of antimicrobial resistance genes in four environments: effluent of three municipal wastewater treatment facilities, three cattle feedlot runoff catchment ponds, three swine waste lagoons, and two "low impact" environments (an urban lake and a relict prairie). Multiple liquid and solid samples were collected from each environment. The prevalences and concentrations of antimicrobial-resistant (AMR) Gram-negative (Escherichia coli and Salmonella enterica) and Gram-positive (enterococci) bacteria were determined from individual samples (n = 174). The prevalences of 84 antimicrobial resistance genes in metagenomic DNA isolated from samples pooled (n = 44) by collection date, location, and sample type were determined. The prevalences and concentrations of AMR E. coli and Salmonella were similar among the livestock and municipal sample sources. The levels of erythromycin-resistant enterococci were significantly higher in liquid samples from cattle catchment ponds and swine waste lagoons than in liquid samples from municipal wastewater treatment facilities, but solid samples from these environments did not differ significantly. Similarly, trimethoprim/sulfamethoxazole-resistant E. coli concentrations were significantly higher in swine liquid than in municipal liquid samples, but there was no difference in solid samples. Multivariate analysis of the distribution of antimicrobial resistance genes using principal coordinate analysis showed distinct clustering of samples with livestock (cattle and swine), low impact environment and municipal samples forming three separate clusters. The numbers of class A beta-lactamase, class C beta-lactamase, and fluoroquinolone resistance genes detected were significantly higher (P < 0.05) in municipal samples than in cattle runoff or swine lagoon samples. In conclusion, we report that AMR is a very widespread phenomenon and that similar prevalences and concentrations of antimicrobial-resistant bacteria and antimicrobial resistance genes exist in cattle, human, and swine waste streams, but a higher diversity of antimicrobial resistance genes are present in treated human waste discharged from municipal wastewater treatment plants than in livestock environments. C1 [Agga, Getahun E.; Arthur, Terrance M.; Harhay, Dayna M.; Schmidt, John W.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Durso, Lisa M.] ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE USA. RP Schmidt, JW (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Terrance.Arthur@ars.usda.gov FU Beef Checkoff [58-5438-3-414]; United States Department of Agriculture-Agricultural Research Service [5438-42000-014-00] FX This project was funded in part by the Beef Checkoff (#58-5438-3-414; to TMA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The remainder of the funding was provided by internal project funding from United States Department of Agriculture-Agricultural Research Service, Project Number 5438-42000-014-00. NR 73 TC 7 Z9 7 U1 8 U2 58 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 21 PY 2015 VL 10 IS 7 AR e0132586 DI 10.1371/journal.pone.0132586 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN6LZ UT WOS:000358547600041 PM 26197056 ER PT J AU Cade-Menun, BJ He, ZQ Dou, ZX AF Cade-Menun, Barbara J. He, Zhongqi Dou, Zhengxia TI Comparison of Phosphorus Forms in Three Extracts of Dairy Feces by Solution P-31 NMR Analysis SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE excreta; phosphorus; extraction; dithionite; Animal manure ID NUCLEAR-MAGNETIC-RESONANCE; ORGANIC PHOSPHORUS; POTENTIAL BIOAVAILABILITY; ANIMAL MANURES; POULTRY LITTER; SPECTROSCOPY; SOIL; SPECIATION; IDENTIFICATION; SOLUBILITY AB Using solution P-31 NMR spectroscopy, we compared three extractants, deionized water, sodium acetate buffer (pH 5.0) with fresh sodium dithionite (NaAc-SD), and 0.25M NaOH-0.05M ethylenediaminetetraacetic acid (NaOH-EDTA), for the profile of P compounds in two dairy fecal samples. Phosphorus extracted was 35 percent for water and 80-100 percent for NaAc-SD and NaOH-EDTA. Water extracted a greater proportion of organic P forms, particularly orthophosphate diesters, but a lower proportion of orthophosphate relative to NaOH-EDTA and NaAc-SD. Phosphorus recoveries and spectra were generally similar for NaOH-EDTA and NaAc-SD, but there was increased degradation of polyphosphate to pyrophosphate with NaOH-EDTA, and water, for one sample relative to NaAc-SD. For studies characterizing and quantifying all the P forms in manure and fecal samples, these results suggest that either NaOH-EDTA or NaAc-SD would be a good choice. However, extraction with water may be a better choice for studies monitoring labile manure P and potential environmental impact. C1 [Cade-Menun, Barbara J.] Agr & Agri Food Canada, Semiarid Prairie Agr Res Ctr, Swift Current, SK, Canada. [He, Zhongqi] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Dou, Zhengxia] Univ Penn, Sch Vet Med, Sect Anim Prod Syst, Kennett Sq, PA 19348 USA. RP He, ZQ (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. EM Zhongqi.He@ars.usda.gov FU USDA CSREES IFAFS [2001-52103-11334] FX This research was funded in part by USDA CSREES IFAFS Program Grant 2001-52103-11334. NR 29 TC 5 Z9 5 U1 3 U2 13 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0010-3624 EI 1532-2416 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD JUL 20 PY 2015 VL 46 IS 13 BP 1698 EP 1712 DI 10.1080/00103624.2015.1047512 PG 15 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA CN0UX UT WOS:000358130500008 ER PT J AU Clifton, EH Jaronski, ST Hodgson, EW Gassmann, AJ AF Clifton, Eric H. Jaronski, Stefan T. Hodgson, Erin W. Gassmann, Aaron J. TI Abundance of Soil-Borne Entomopathogenic Fungi in Organic and Conventional Fields in the Midwestern USA with an Emphasis on the Effect of Herbicides and Fungicides on Fungal Persistence SO PLOS ONE LA English DT Article ID METARHIZIUM-ANISOPLIAE; BEAUVERIA-BASSIANA; FARMING SYSTEM; AGRICULTURE; MARGINS; INSECT; BIODIVERSITY; ENVIRONMENT; MANAGEMENT; PHYLOGENY AB Entomopathogenic fungi (EPF) are widespread in agricultural fields and help suppress crop pests. These natural enemies may be hindered by certain agronomic practices associated with conventional agriculture including the use of pesticides. We tested whether the abundance of EPF differed between organic and conventional fields, and whether specific cropping practices and soil properties were correlated with their abundance. In one year of the survey, soil from organic fields and accompanying margins had significantly more EPF than conventional fields and accompanying margins. Regression analysis revealed that the percentage of silt and the application of organic fertilizer were positively correlated with EPF abundance; but nitrogen concentration, tillage, conventional fields, and margins of conventional fields were negatively correlated with EPF abundance. A greenhouse experiment in which fungicides and herbicides were applied to the soil surface showed no significant effect on EPF. Though organic fields were perceived to be more suitable environments for EPF, abiotic factors and cropping practices such as tillage may have greater impacts on the abundance of EPF. Also, fungicides and herbicides may not be as toxic to soil-borne EPF as originally thought. C1 [Clifton, Eric H.; Hodgson, Erin W.; Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Jaronski, Stefan T.] USDA, Northern Plains Agr Res Lab, Sidney, MT USA. RP Clifton, EH (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM eclifton@iastate.edu FU Leopold Center for Sustainable Agriculture [E2010-18] FX This work was supported by Leopold Center for Sustainable Agriculture, Grant: E2010-18 [http://www.leopold.iastate.edu/grants/e2010-18]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 0 Z9 0 U1 3 U2 34 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 20 PY 2015 VL 10 IS 7 AR e0133613 DI 10.1371/journal.pone.0133613 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN6LN UT WOS:000358546400106 PM 26191815 ER PT J AU Setamou, M Bartels, DW AF Setamou, Mamoudou Bartels, David W. TI Living on the Edges: Spatial Niche Occupation of Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), in Citrus Groves SO PLOS ONE LA English DT Article ID HOST PLANTS; DISEASE; VECTOR; OVIPOSITION; TEMPERATURE; MANAGEMENT; RESPONSES; PATTERNS; BIOLOGY; FLORIDA AB The spatial niche occupation of the Asian citrus psyllid, Diaphorina citri Kuwayama, 1908, was evaluated to determine its field colonization and food resource exploitation strategies in citrus groves. Mature grapefruit and sweet orange groves were surveyed as part of an areawide program in 2009-2010 to determine D. citri population densities and between-tree distribution. In both cultivars, significantly more psyllids were found on perimeter trees throughout the study period suggesting a strong edge effect in D. citri distribution in the groves. D. citri densities and infestation levels gradually declined from the edge to the center of grove. Higher numbers of D. citri were recorded on trees located on the east and south sides of the groves than those on the west and north sides. Citrus groves located at the outer edge of the study with at least one side non-surrounded to other citrus groves harbored significantly more D. citri than groves located within the block cluster and entirely surrounded by other groves. In detailed field studies during 2012, infestation of D. citri started from border trees in the grove where possibly one generation is completed before inner trees become infested. In addition, psyllid densities decreased significantly with increasing distance from the grove edge. Using the selection index, D citri exhibited a strong niche occupation preference for border trees. C1 [Setamou, Mamoudou] Texas A&M Univ, Dept Agr Agribusiness & Environm Sci, Kingsville Citrus Ctr, Weslaco, TX 78599 USA. [Bartels, David W.] USDA APHIS, Ctr Plant Hlth Sci Technol, Plant Protect Quarantine Miss Lab, Mission, TX USA. RP Setamou, M (reprint author), Texas A&M Univ, Dept Agr Agribusiness & Environm Sci, Kingsville Citrus Ctr, Weslaco, TX 78599 USA. EM mamoudou.setamou@tamuk.edu FU United States Department of Agriculture, Animal and Plant Health Inspection Services, Plant Protection and Quarantine [09-8412-1047CA] FX This study was supported by the United States Department of Agriculture, Animal and Plant Health Inspection Services, Plant Protection and Quarantine (09-8412-1047CA). NR 33 TC 4 Z9 4 U1 3 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 20 PY 2015 VL 10 IS 7 AR e0131917 DI 10.1371/journal.pone.0131917 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN6LN UT WOS:000358546400017 PM 26193111 ER PT J AU Skinner, C Patfield, S Hernlem, BJ He, XH AF Skinner, Craig Patfield, Stephanie Hernlem, Bradley J. He, Xiaohua TI New Stx2e Monoclonal Antibodies for Immunological Detection and Distinction of Stx2 Subtypes SO PLOS ONE LA English DT Article ID SHIGA TOXIN 2; ESCHERICHIA-COLI; EDEMA DISEASE; NEUTRALIZATION; VARIANTS; HUMANS; PURIFICATION; SUBUNIT; CELLS; ASSAY AB Background Stx2e is a primary virulence factor in STEC strains that cause edema disease in neonatal piglets. Though Stx2a and Stx2e are similar, many antibody-based Stx detection kits are designed to detect Stx2a and do not recognize the Stx2e subtype. Methods and Findings Four monoclonal antibodies against Stx2e were developed and characterized. Two of these mAbs recognize the B subunit of Stx2e, Stx2f, and to a lesser extent, Stx2b, Stx2c, and Stx2d. The other two mAbs recognize the A subunit of Stx2e, and cross-react with all Stx2 subtypes except Stx2f. The most sensitive sandwich ELISA using these mAbs has a limit of detection for Stx2e of 11.8 pg/mL. The ability of the neutralizing antibody Stx2e-2 to block Stx2e-receptor binding in Vero cells was visualized using immunofluorescence. Combinations of these and previously developed mAbs permit ELISA-based differentiation between closely related Stx2a, Stx2c, and Stx2d (using mAbs Stx2-5/2-1, Stx2-5/2e-2, and Stx2e-3/2e-2, respectively). Conclusions The sensitive immunoassays developed in this study should augment our capacity to detect Stx2e in porcine environments and biological samples. Moreover, immunoassays that can distinguish between the closely related Stx2a, Stx2c, and Stx2d subtypes can be useful in quickly analyzing Stx subtypes in samples containing more than one strain of STEC. C1 [Skinner, Craig; Patfield, Stephanie; Hernlem, Bradley J.; He, Xiaohua] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP He, XH (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM xiaohua.he@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS) National Program Current Research Information System (CRIS) [5325-42000-048-00D, NP108] FX This study was funded by the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) National Program NP108 Current Research Information System (CRIS) project 5325-42000-048-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 28 TC 3 Z9 3 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 20 PY 2015 VL 10 IS 7 AR e0132419 DI 10.1371/journal.pone.0132419 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN6LN UT WOS:000358546400025 PM 26192407 ER PT J AU Wang, J Hua, LS Pan, H Zhang, LZ Li, MX Huang, YZ Li, ZJ Lan, XY Lei, CZ Li, CJ Chen, H AF Wang, Jing Hua, Liu-shuai Pan, Hong Zhang, Liang-zhi Li, Ming-xun Huang, Yong-zhen Li, Zhuan-jian Lan, Xian-yong Lei, Chu-zhao Li, Cong-jun Chen, Hong TI Haplotypes in the promoter region of the CIDEC gene associated with growth traits in Nanyang cattle SO SCIENTIFIC REPORTS LA English DT Article ID DFF45-LIKE EFFECTOR-C; BROWN ADIPOSE-TISSUE; CELL-DEATH; HUMAN ADIPOCYTES; LIPID DROPLETS; DEFICIENT MICE; FSP27; METABOLISM; WHITE; IDENTIFICATION AB Cell death-inducing DFFA-like effector c (CIDEC, also known as Fsp27) has emerged as an important regulator of metabolism associated with lipodystrophy, diabetes, and hepatic steatosis. It is required for unilocular lipid droplet formation and optimal energy storage. The mechanism between this gene and livestock growth traits, however, has yet to be reported. In this study, we found ten novel single nucleotide polymorphisms (SNPs) in the 5' transcriptional region of CIDEC in Nanyang (NY) cattle, which are located in the recognition sequences (potential cis-acting elements) of 22 transcription factors, and the nine haplotypes represent nine different combinations of polymorphic potential cisacting elements. The results indicated that individuals with the H8-H8 diplotype had heavier body weights and faster growth rates (P < 0.01) at 18th months than those with H1-H8. We evaluated the transcriptional activities of different haplotypes in vitro, the results were consistent with the association analysis. The H8 haplotype had 1.88-fold (P < 0.001) higher transcriptional activity than the H1 haplotype. We speculate that the haplotypes of the potential cis-acting elements may affect the transcriptional activity of CIDEC, thus affecting the growth traits of cattle. This information may be used in molecular marker-assisted selection of cattle breeding in the future. C1 [Wang, Jing; Pan, Hong; Zhang, Liang-zhi; Li, Ming-xun; Huang, Yong-zhen; Li, Zhuan-jian; Lan, Xian-yong; Lei, Chu-zhao; Chen, Hong] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China. [Wang, Jing; Hua, Liu-shuai] Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450002, Henan, Peoples R China. [Li, Cong-jun] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. RP Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China. EM chenhong1212@263.net FU National Natural Science Foundation of China [31272408]; National 863 Program of China [2013AA102505]; Program of National Beef Cattle and Yak Industrial Technology System [CARS-38] FX This study was supported by National Natural Science Foundation of China (Grant No. 31272408), the National 863 Program of China (Grant No. 2013AA102505), Program of National Beef Cattle and Yak Industrial Technology System (Grant No. CARS-38). We thank Dr. Wei Chen (Department of Evolutionary Ecology and Genetics, Zoological Institute, Christian Albrechts-Universitaet zu Kiel, Germany) for language editing of the manuscript. NR 24 TC 2 Z9 2 U1 0 U2 9 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 20 PY 2015 VL 5 AR 12075 DI 10.1038/srep12075 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1HR UT WOS:000358169900001 PM 26189824 ER PT J AU Ge, LQ Jiang, YP Xia, T Song, QS Stanley, D Kuai, P Lu, XL Yang, GQ Wu, JC AF Ge, Lin-Quan Jiang, Yi-Ping Xia, Ting Song, Qi-Sheng Stanley, David Kuai, Peng Lu, Xiu-Li Yang, Guo-Qing Wu, Jin-Cai TI Silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Delphacidae) SO SCIENTIFIC REPORTS LA English DT Article ID PHLOEM-FEEDING INSECT; ADULT FEMALES; BODY-SIZE; INTRASPECIFIC VARIATION; ACYRTHOSIPHON-PISUM; VITELLIN CONTENT; EXPRESSION; LEPIDOPTERA; DIET; SUPPRESSION AB The brown planthopper (BPH), Nilaparvata lugens, sugar transporter gene 6 (Nlst6) is a facilitative glucose/fructose transporter (often called a passive carrier) expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. The influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. Nonetheless, it is reasonable to suspect that transporter-mediated uptake of dietary sugar is essential to the biology of phloem-feeding insects. Based on this reasoning, we posed the hypothesis that silencing, or reducing expression, of a BPH sugar transporter gene would be deleterious to the insects. To test our hypothesis, we examined the effects of Nlst6 knockdown on BPH biology. Reducing expression of Nlst6 led to profound effects on BPHs. It significantly prolonged the pre-oviposition period, shortened the oviposition period, decreased the number of eggs deposited and reduced body weight, compared to controls. Nlst6 knockdown also significantly decreased fat body and ovarian (particularly vitellogenin) protein content as well as vitellogenin gene expression. Experimental BPHs accumulated less fat body glucose compared to controls. We infer that Nlst6 acts in BPH growth and fecundity, and has potential as a novel target gene for control of phloem-feeding pest insects. C1 [Ge, Lin-Quan; Jiang, Yi-Ping; Xia, Ting; Kuai, Peng; Lu, Xiu-Li; Yang, Guo-Qing; Wu, Jin-Cai] Yangzhou Univ, Sch Plant Protect, Yangzhou 225009, Peoples R China. [Song, Qi-Sheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. RP Wu, JC (reprint author), Yangzhou Univ, Sch Plant Protect, Yangzhou 225009, Peoples R China. EM jincaiwu1952@sina.com FU National Natural Science Foundation of China [31201507]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [12KJB210006]; College Students Practice Innovation Training Program of Jiangsu Province University [201311117033Z]; major state Basic Research Program of China (973 program) [2010CB126200] FX This research was supported in part by the National Natural Science Foundation of China (31201507), by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (12KJB210006), by the College Students Practice Innovation Training Program of Jiangsu Province University (201311117033Z), by the major state Basic Research Program of China (973 program, Grant No. 2010CB126200). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 34 TC 3 Z9 3 U1 5 U2 40 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 17 PY 2015 VL 5 AR 12194 DI 10.1038/srep12194 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CM9OH UT WOS:000358037600001 PM 26185058 ER PT J AU Vano, JA Kim, JB Rupp, DE Mote, PW AF Vano, Julie A. Kim, John B. Rupp, David E. Mote, Philip W. TI Selecting climate change scenarios using impact-relevant sensitivities SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article DE sensitivity analysis; hydrologic modeling; vegetation carbon modeling; global climate model ranking; Pacific Northwest ID UNITED-STATES; PRECIPITATION; TEMPERATURE; ASSESSMENTS; PROJECTION; BASIN AB Climate impact studies often require the selection of a small number of climate scenarios. Ideally, a subset would have simulations that both (1) appropriately represent the range of possible futures for the variable/s most important to the impact under investigation and (2) come from global climate models (GCMs) that provide plausible results for future climate in the region of interest. We demonstrate an approach to select a subset of GCMs that incorporates both concepts and provides insights into the range of climate impacts. To represent how an ecosystem process responds to projected future changes, we methodically sample, using a simple sensitivity analysis, how an ecosystem variable responds locally to projected regional temperature and precipitation changes. We illustrate our approach in the Pacific Northwest, focusing on (a) changes in streamflow magnitudes in critical seasons for water management and (b) changes in annual vegetation carbon. C1 [Vano, Julie A.; Rupp, David E.; Mote, Philip W.] Oregon State Univ, Oregon Climate Change Res Inst, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Kim, John B.] US Forest Serv, Pacific NW Res Stn, Prineville, OR USA. [Kim, John B.] US Forest Serv, Western Wildland Environm Threat Assessment Ctr, Prineville, OR USA. RP Vano, JA (reprint author), Oregon State Univ, Oregon Climate Change Res Inst, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. EM jvano@coas.oregonstate.edu RI Rupp, David/G-8171-2014; OI Kim, John/0000-0002-3720-7916 FU National Science Foundation (NSF) [EAR-1250087, EAR-1039192]; U.S. National Oceanic and Atmospheric Administration (NOAA) [NA10OAR4310218]; Department of the Interior Northwest Climate Science Center (NW CSC) from the United States Geological Survey (USGS) [G12AC20495] FX The authors thank two anonymous reviewers for their feedback and G. Stephen Pitts for technical assistance with running MC2 simulations. GCM precipitation and temperature changes were calculated from data downloaded at http://cmip-pcmdi.llnl.gov/cmip5. This material is based upon the work supported by the National Science Foundation (NSF) under awards EAR-1250087 and EAR-1039192, by the U.S. National Oceanic and Atmospheric Administration (NOAA), grant NA10OAR4310218, and by the Department of the Interior Northwest Climate Science Center (NW CSC) through a cooperative agreement (G12AC20495) from the United States Geological Survey (USGS). Its contents are solely the responsibility of the authors and do not necessarily represent the views of the NSF, NOAA, NW CSC, or the USGS. This manuscript is submitted for publication with the understanding that the United States Government is authorized to reproduce and distribute reprints for governmental purposes. NR 33 TC 3 Z9 3 U1 2 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD JUL 16 PY 2015 VL 42 IS 13 BP 5516 EP 5525 DI 10.1002/2015GL063208 PG 10 WC Geosciences, Multidisciplinary SC Geology GA CN8LG UT WOS:000358691300054 ER PT J AU Kornelsen, KC Cosh, MH Coulibaly, P AF Kornelsen, Kurt C. Cosh, Michael H. Coulibaly, Paulin TI Potential of bias correction for downscaling passive microwave and soil moisture data SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE bias correction; downscaling; soil moisture; CMEM ID SYNTHETIC-APERTURE RADAR; TEMPORAL STABILITY; DATA ASSIMILATION; DATA SETS; VALIDATION; EMISSION; MODEL; RADIOMETER; RETRIEVAL; ALGORITHM AB Passive microwave satellites such as Soil Moisture and Ocean Salinity or Soil Moisture Active Passive observe brightness temperature (TB) and retrieve soil moisture at a spatial resolution greater than most hydrological processes. Bias correction is proposed as a simple method to disaggregate soil moisture to a scale more appropriate for hydrological applications. Temporal stability of soil moisture and TB was demonstrated at the Little Washita and Little River Experimental Watersheds using in situ observations and the Community Microwave Emissions Model. Decomposition of the mean square difference (MSD) between the watershed average soil moisture and TB showed that bias was a major contributor to differences between watershed average and local-scale soil moisture and TB, particularly at sites with high MSD. The mean RMSD between watershed average and local soil moisture was 0.04m(3)m(-3) and 0.06m(3)m(-3) at Little River and Little Washita, respectively. Following a simple bias correction the RMSD was reduced to 0.03m(3)m(-3) at both sites. Considering multiple incidence angles at both horizontal and vertical polarization, bias correction of watershed average TBV reduced the RMSD by approximately 75% and 45% and TBH RMSD by 68% and 36% for Little River and Little Washita, respectively, at all incidence angles. Therefore, at subsatellite grid scale, bias correction can be considered a viable technique for downscaling passive microwave observations and soil moisture retrievals. C1 [Kornelsen, Kurt C.; Coulibaly, Paulin] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON, Canada. [Cosh, Michael H.] ARS, USDA, Hydrol & Remote Lab, Beltsville, MD USA. [Coulibaly, Paulin] McMaster Univ, Dept Civil Engn, Hamilton, ON, Canada. RP Kornelsen, KC (reprint author), McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON, Canada. EM kornelkc@mcmaster.ca FU Natural Science and Engineering Research Council of Canada FX USDA-ARS data are currently available via each research laboratory (USDA-ARS Grazinglands Research Laboratory in El Reno, OK, or USDA-ARS-Southeast Watershed Research Laboratory in Tifton, GA. Meteorological data from the USDA SCAN sites were downloaded from (http://www.wcc.nrcs.usda.gov/scan/). The soil texture data are obtained from the SMEX03 that are freely available from the NASA DAAC at NSIDC (http://nsidc.org/data/amsr_validation/soil_moisture/smex03/). MODIS LAI data were accessed from the NASA Land Processes DAAC available at https://lpdaac.usgs.gov/. The MODIS land cover climatology data used were accessed via the USGS Land Cover Institute at http://landcover.usgs.gov/global_climatology.php. The Community Microwave Emission Model can be downloaded from the ECMWF at https://software.ecmwf.int/wiki/display/LDAS/CMEM. We would like to thank the Natural Science and Engineering Research Council of Canada for research funding. We are grateful for the contribution of three anonymous reviewers whose comments and suggestions have helped improve this manuscript. NR 64 TC 4 Z9 4 U1 0 U2 13 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD JUL 16 PY 2015 VL 120 IS 13 BP 6460 EP 6479 DI 10.1002/2015JD023550 PG 20 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CN8MN UT WOS:000358695200010 ER PT J AU Olson, MR Garcia, MV Robinson, MA Van Rooy, P Dietenberger, MA Bergin, M Schauer, JJ AF Olson, Michael R. Garcia, Mercedes Victoria Robinson, Michael A. Van Rooy, Paul Dietenberger, Mark A. Bergin, Michael Schauer, James Jay TI Investigation of black and brown carbon multiple-wavelength-dependent light absorption from biomass and fossil fuel combustion source emissions SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE black carbon; brown carbon; aethalometer; emission sources; absorption coefficient ID PRIMARY PARTICLE EMISSIONS; PHOTOACOUSTIC SPECTROMETER; SOURCE APPORTIONMENT; PARTICULATE MATTER; OPTICAL-ABSORPTION; AETHALOMETER DATA; ELEMENTAL CARBON; 7-WAVELENGTH AETHALOMETER; ANGSTROM EXPONENT; SOOT PARTICLES AB Quantification of the black carbon (BC) and brown carbon (BrC) components of source emissions is critical to understanding the impact combustion aerosols have on atmospheric light absorption. Multiple-wavelength absorption was measured from fuels including wood, agricultural biomass, coals, plant matter, and petroleum distillates in controlled combustion settings. Filter-based absorption measurements were corrected and compared to photoacoustic absorption results. BC absorption was segregated from the total light extinction to estimate the BrC absorption from individual sources. Results were compared to elemental carbon (EC)/organic carbon (OC) concentrations to determine composition's impact on light absorption. Multiple-wavelength absorption coefficients, Angstrom exponent (6.9 to <1.0), mass absorption cross section (MAC), and Delta C (97 mu gm(-3) to 0 mu gm(-3)) were highly variable. Sources such as incense and peat emissions showed ultraviolet wavelength (370nm) BrC absorption over 175 and 80 times (respectively) the BC absorption but only 21 and 11 times (respectively) at 520nm wavelength. The bulk EC MAC(EC,) (average at 520nm=9.03.7m(2)g(-1); with OC fraction <0.85=7.5m(2)g(-1)) and the BrC OC mass absorption cross sections (MAC(BrC,OC,)) were calculated; at 370nm ultraviolet wavelengths; the MAC(BrC,OC,) ranged from 0.8m(2)g(-1) to 2.29m(2)g(-1) (lowest peat, highest kerosene), while at 520nm wavelength MAC(BrC,OC,) ranged from 0.07m2g(-1) to 0.37m(2)g(-1) (lowest peat, highest kerosene/incense mixture). These MAC results show that OC content can be an important contributor to light absorption when present in significant quantities (>0.9 OC/TC), source emissions have variable absorption spectra, and nonbiomass combustion sources can be significant contributors to BrC. C1 [Olson, Michael R.; Garcia, Mercedes Victoria; Van Rooy, Paul; Schauer, James Jay] Univ Wisconsin, Environm Chem & Technol Program, Madison, WI 53706 USA. [Robinson, Michael A.] Cummins Emiss Solut, Stoughton, WI USA. [Dietenberger, Mark A.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. [Bergin, Michael] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA. RP Schauer, JJ (reprint author), Univ Wisconsin, Environm Chem & Technol Program, Madison, WI 53706 USA. EM jjschauer@wisc.edu FU U.S. EPA grant [R835039] FX This publication was made possible by U.S. EPA grant (R835039). Its contents are solely the responsibility of the grantee and do not necessarily represent the official views of the U.S. EPA. Further, U.S. EPA does not endorse the purchase of any commercial products or services mentioned in the publication. In addition, we would like to thank the following individuals and entities for their support of this research: Cummins Emission Solutions, Stoughton WI; USDA Forest Products Research Laboratory, Madison, WI; Wisconsin State Laboratory of Hygiene, Madison, WI, for chemical analysis support; Ken Ragland UW-Madison Professor Emeritus for expertise in solid fuel combustion, Charles Boardman USFS for sampling support; and the following UW-System graduate and undergraduate researchers: Charlie Feit, Jerome McGinnis, Ying Zhan, Austin Yantes, Jongbae Heo, Kristin Short, and Rebecca Goldberg. Data used to develop the figures and addition results not specifically discussed in the manuscript are reported in the supporting information. All results reported in this paper are available to readers at the NERSC repository http://www.nersc.gov/users/science-gateways/. NR 64 TC 12 Z9 12 U1 14 U2 78 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD JUL 16 PY 2015 VL 120 IS 13 BP 6682 EP 6697 DI 10.1002/2014JD022970 PG 16 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CN8MN UT WOS:000358695200023 ER PT J AU Arevalo-Gardini, E Canto, M Alegre, J Loli, O Julca, A Baligar, V AF Arevalo-Gardini, Enrique Canto, Manuel Alegre, Julio Loli, Oscar Julca, Alberto Baligar, Virupax TI Changes in Soil Physical and Chemical Properties in Long Lerm Improved Natural and Traditional Agroforestry Management Systems of Cacao Genotypes in Peruvian Amazon SO PLOS ONE LA English DT Article ID LAND-CLEARING METHOD; FOREST SOILS; ORGANIC-MATTER; SHORT-TERM; BURN AGRICULTURE; CROP PRODUCTION; FIRE; AVAILABILITY; RHIZOSPHERE; FERTILITY AB Growing cacao (Theobroma cacao L.) in an agroforestry system generates a productive use of the land, preserves the best conditions for physical, chemical and biological properties of tropical soils, and plays an important role in improving cacao production and fertility of degraded tropical soils. The aim of this study was to evaluate the impact of two long term agroforestry systems of cacao management on soil physical and chemical properties in an area originally inhabited by 30 years old native secondary forest (SF). The two agroforestry systems adapted were: improved natural agroforestry system (INAS) where trees without economic value were selectively removed to provide 50% shade and improved traditional agroforestry system (ITAS) where all native trees were cut and burnt in the location. For evaluation of the changes of soil physical and chemical properties with time due to the imposed cacao management systems, plots of 10 cacao genotypes (ICS95, UF613, CCN51, ICT1112, ICT1026, ICT2162, ICT2171, ICT2142, H35, U30) and one plot with a spontaneous hybrid were selected. Soil samples were taken at 0-20, 20-40 and 40-60 cm depths before the installation of the management systems (2004), and then followed at two years intervals. Bulk density, porosity, field capacity and wilting point varied significantly during the years of assessment in the different soil depths and under the systems assessed. Soil pH, CEC, exchangeable Mg and sum of the bases were higher in the INAS than the ITAS. In both systems, SOM, Ext. P, K and Fe, exch. K, Mg and Al+H decreased with years of cultivation; these changes were more evident in the 0-20 cm soil depth. Overall improvement of SOM and soil nutrient status was much higher in the ITAS than INAS. The levels of physical and chemical properties of soil under cacao genotypes showed a marked difference in both systems. C1 [Arevalo-Gardini, Enrique] ICT, Tarapoto, Peru. [Canto, Manuel; Alegre, Julio; Loli, Oscar; Julca, Alberto] UNALM, Lima, Peru. [Baligar, Virupax] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Arevalo-Gardini, E (reprint author), ICT, Tarapoto, Peru. EM enriquearevaloga@gmail.com FU Instituto de Cultivos Tropicales (ICT); United States-Department of Agriculture-Agricultural Research Service (USDA-ARS); United States Embassy (INL) in Lima-Peru FX This study was supported by Instituto de Cultivos Tropicales (ICT), United States-Department of Agriculture-Agricultural Research Service (USDA-ARS) and the United States Embassy (INL) in Lima-Peru. NR 94 TC 3 Z9 3 U1 4 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 16 PY 2015 VL 10 IS 7 AR e0132147 DI 10.1371/journal.pone.0132147 PG 29 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1RQ UT WOS:000358198200016 PM 26181053 ER PT J AU Venterea, RT Clough, TJ Coulter, JA Breuillin-Sessoms, F AF Venterea, Rodney T. Clough, Timothy J. Coulter, Jeffrey A. Breuillin-Sessoms, Florence TI Ammonium sorption and ammonia inhibition of nitrite-oxidizing bacteria explain contrasting soil N2O production SO SCIENTIFIC REPORTS LA English DT Article ID NITROUS-OXIDE PRODUCTION; NITRIFIER DENITRIFICATION; AGRICULTURAL SOIL; GRAZED PASTURE; NITRIFICATION; EMISSIONS; ARCHAEA; OXIDATION; KINETICS; ACID AB Better understanding of process controls over nitrous oxide (N2O) production in urine-impacted 'hot spots' and fertilizer bands is needed to improve mitigation strategies and emission models. Following amendment with bovine (Bos taurus) urine (Bu) or urea (Ur), we measured inorganic N, pH, N2O, and genes associated with nitrification in two soils ('L' and 'W') having similar texture, pH, C, and C/N ratio. Solution-phase ammonia (slNH(3)) was also calculated accounting for non-linear ammonium (NH4+) sorption capacities (ASC). Soil W displayed greater nitrification rates and nitrate (NO3-) levels than soil L, but was more resistant to nitrite (NO2-) accumulation and produced two to ten times less N2O than soil L. Genes associated with NO2- oxidation (nxrA) increased substantially in soil W but remained static in soil L. Soil NO2- was strongly correlated with N2O production, and cumulative (c-) slNH(3) explained 87% of the variance in c-NO2-. Differences between soils were explained by greater slNH(3) in soil L which inhibited NO2- oxidization leading to greater NO2- levels and N2O production. This is the first study to correlate the dynamics of soil slNH(3), NO2-, N2O and nitrifier genes, and the first to show how ASC can regulate NO2- levels and N2O production. C1 [Venterea, Rodney T.] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Venterea, Rodney T.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Clough, Timothy J.] Lincoln Univ, Fac Agr & Life Sci, Canterbury 7647, New Zealand. [Coulter, Jeffrey A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Breuillin-Sessoms, Florence] Univ Minnesota, Coll Biol Sci, Inst Biotechnol, St Paul, MN 55108 USA. RP Venterea, RT (reprint author), USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. EM Venterea@umn.edu RI Venterea, Rodney/A-3930-2009 FU Global Research Alliance Senior Scientist (GRASS) fellowship FX The authors gratefully acknowledge the assistance of S. Mitchell, P. Wang and M. Sadowsky in establishing the PCR methods, and S. Khan, S. Mitchell, K. Sidla and M. Dolan in assisting with laboratory analysis. This work was supported by a Global Research Alliance Senior Scientist (GRASS) fellowship to R. Venterea and T. Clough from the Livestock Emissions and Abatement Research Network. NR 48 TC 11 Z9 11 U1 14 U2 69 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 16 PY 2015 VL 5 AR 12153 DI 10.1038/srep12153 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CM9DP UT WOS:000358007100002 PM 26179972 ER PT J AU Wu, SF Xu, G Stanley, D Huang, J Ye, GY AF Wu, Shun-Fan Xu, Gang Stanley, David Huang, Jia Ye, Gong-Yin TI Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis SO SCIENTIFIC REPORTS LA English DT Article ID DENDRITIC CELLS; IMMUNOREGULATORY ROLE; OCTOPAMINE RECEPTOR; MOLECULAR-CLONING; INNATE IMMUNITY; MUSHROOM BODIES; APIS-MELLIFERA; MANDUCA-SEXTA; DRUG-ABUSE; DROSOPHILA AB Dopamine (DA) is a signal moiety bridging the nervous and immune systems. DA dysregulation is linked to serious human diseases, including addiction, schizophrenia, and Parkinson's disease. However, DA actions in the immune system remain incompletely understood. In this study, we found that DA modulates insect hemocyte phagocytosis using hemocytes prepared from the rice stem borer (RSB), Chilo suppressalis. We investigated whether insect hemocytes are capable of de novo DA production. Here we show that exposing hemocytes to lipopolysaccharide (LPS) led to induction of DA-generating enzymes. Exogenous DA induced rapid phosphorylation of extracellular signal-regulated kinase (ERK) in naive hemocytes. Activation of ERK was inhibited by preincubating with a DOP1 receptor antagonist. Thus, DA signaling via the DOP1 receptor may contribute to early hemocyte activation. DA synthesized and released from hemocytes may act in an autocrine mechanism to stimulate or maintain phagocytic activity. Consistent with this hypothesis, we found that inhibition of DA synthesis with alpha-methyl-DL-tyrosine methyl ester hydrochloride or blockage of DOP1 receptor with antagonist SCH23390 impaired hemocyte phagocytosis. Topical DA application also significantly decreased RSB mortality following challenge with the insect pathogenic fungus, Beauveria bassiana. We infer that a DA-dependent signaling system operates in hemocytes to mediate phagocytotic functions. C1 [Wu, Shun-Fan; Xu, Gang; Huang, Jia; Ye, Gong-Yin] Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China. [Wu, Shun-Fan; Xu, Gang; Huang, Jia; Ye, Gong-Yin] Zhejiang Univ, Inst Insect Sci, Key Lab Agr Entomol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China. [Wu, Shun-Fan] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China. [Wu, Shun-Fan] State & Local Joint Engn Res Ctr Green Pesticide, Nanjing, Jiangsu, Peoples R China. [Stanley, David] USDA ARS, BCIRL, Columbia, MO 65203 USA. RP Huang, J (reprint author), Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China. EM huangj@zju.edu.cn; chu@zju.edu.cn FU National Program on Key Basic Research Projects (973 Program) [2013CB127600]; China National Science Fund for Innovative Research Groups of Biological Control [31321063]; National Natural Science Foundation of China [31401782]; China National Science Fund for Distinguished Young Scholars [31025021] FX We are grateful to Dr. L.S. Song (Institute of Oceanology, Chinese Academy of Sciences) for sharing antibodies. We thank Y.Q. Li and X.D. Wu for the confocal microscopy and LC-MS/MS service for technical assistance. This work was financed by National Program on Key Basic Research Projects (973 Program, 2013CB127600), China National Science Fund for Innovative Research Groups of Biological Control (Grant no. 31321063), National Natural Science Foundation of China (31401782) and China National Science Fund for Distinguished Young Scholars (Grant no. 31025021). NR 53 TC 4 Z9 4 U1 2 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 16 PY 2015 VL 5 AR 12247 DI 10.1038/srep12247 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CM9GH UT WOS:000358014900002 PM 26179416 ER PT J AU DeVetter, L Colquhoun, J Zalapa, J Harbut, R AF DeVetter, Lisa Colquhoun, Jed Zalapa, Juan Harbut, Rebecca TI Yield estimation in commercial cranberry systems using physiological, environmental, and genetic variables SO SCIENTIA HORTICULTURAE LA English DT Article DE Vaccinium macrocarpon; Yield prediction; Regression analysis ID AMERICAN CRANBERRY; VACCINIUM-MACROCARPON; BIENNIAL BEARING; CULTIVARS; HETEROGENEITY; TEMPERATURE AB Improved methods of yield prediction are desired by the cranberry industry for developing early crop pricing forecasts. However, yield is a complex trait that is influenced by multiple interacting factors involving plant physiology, the environment, and cultivar genetics. These factors and their interactions are poorly understood and this ambiguity complicates yield prediction. This study sought to improve the current understanding of yield by measuring the effects of physiological, environmental, and genetics variables on yield. Sixty-six variables were evaluated on 'Stevens' and 'Ben Lear' samples collected from eight commercial cranberry marshes located in Wisconsin during the 2011 and 2012 growing cycles. Regression analysis revealed berry number alone explained 84.5% and 91.3% of the variation associated with yield of 'Stevens' and 'Ben Lear', respectively. Models incorporating berry number and size were accurate at predicting yield (R-2= 0.99 for 'Stevens' and 0.92 for 'Ben Lear'), yet are not useful for early crop forecasting purposes as desired by the industry. Additional regressions done to identify factors that influence berry number revealed large amounts of unexplained variation are associated with this trait. Intracultivar heterogeneity was also found to be substantial across sites and may contribute to the observed variation that could not be explained by the models. Differences in management practices could also contribute to this unexplained variation and is seldom accounted for in yield prediction studies. The main implications of our study suggests that improving the current understanding of variables influencing berry number could have positive implications on future efforts to develop more accurate methods of yield prediction for cranberry. (C) 2015 Elsevier B.V. All rights reserved. C1 [DeVetter, Lisa; Colquhoun, Jed; Zalapa, Juan; Harbut, Rebecca] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Zalapa, Juan] ARS, USDA, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP DeVetter, L (reprint author), Washington State Univ, Mt Vernon Res & Extens Ctr, 16650 State Route 536, Mt Vernon, WA 98273 USA. EM lisa.devetter@wsu.edu; rebecca.harbut@kpu.ca FU Wisconsin State Cranberry Growers Association; Wisconsin Cranberry Board, Inc. FX We would like to acknowledge the Wisconsin State Cranberry Growers Association and the Wisconsin Cranberry Board, Inc., for financial support of this project. Additional acknowledgements are extended to Drs. Beth Workmaster and Carol Barford, the many students who assisted with the project, and cooperating cranberry growers in Wisconsin. Research presented is part of a doctoral thesis and additional acknowledgements are extended to the University of Wisconsin-Madison Department of Horticulture and Dr. DeVetter's graduate committee. NR 22 TC 2 Z9 2 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUL 16 PY 2015 VL 190 BP 83 EP 93 DI 10.1016/j.scienta.2015.04.016 PG 11 WC Horticulture SC Agriculture GA CL2BV UT WOS:000356749500011 ER PT J AU Roh, MS Cheong, EJ Choi, IY Joung, YH AF Roh, Mark S. Cheong, Eun Ju Choi, Ik-Young Joung, Young Hee TI Characterization of wild Prunus yedoensis analyzed by inter-simple sequence repeat and chloroplast DNA (vol 114, pg 121, 2007) SO SCIENTIA HORTICULTURAE LA English DT Correction C1 [Roh, Mark S.] ARS, USDA, Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. [Cheong, Eun Ju] ARS, USDA, Natl Germplasm Resources Lab, Plant Dis Res Unit, Beltsville, MD 20705 USA. [Choi, Ik-Young] Seoul Natl Univ, CALS, NICEM, Seoul 151921, South Korea. [Joung, Young Hee] Cheonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea. RP Cheong, EJ (reprint author), ARS, USDA, Natl Germplasm Resources Lab, Plant Dis Res Unit, Beltsville, MD 20705 USA. EM Mark.Roh@ars.usda.gov; Eunju.Cheong@ars.usda.gov NR 1 TC 1 Z9 1 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUL 16 PY 2015 VL 190 BP 211 EP 211 DI 10.1016/j.scienta.2015.04.001 PG 1 WC Horticulture SC Agriculture GA CL2BV UT WOS:000356749500028 ER PT J AU Etemadnia, H Goetz, SJ Canning, P Tavallali, MS AF Etemadnia, Hamideh Goetz, Stephan J. Canning, Patrick Tavallali, Mohammad Sadegh TI Optimal wholesale facilities location within the fruit and vegetables supply chain with bimodal transportation options: An LP-MIP heuristic approach SO EUROPEAN JOURNAL OF OPERATIONAL RESEARCH LA English DT Article DE Optimal hub location; Bimodal transportation; Operation research; Supply chain ID NETWORK DESIGN; HUB; ALLOCATION; SYSTEM AB Population growth creates a challenge to food availability and access. To balance supply with growing demand, more food has to move from production to consumption sites. Moreover, demand for locally-grown food is increasing and the U.S. Department of Agriculture (USDA) seeks to develop and strengthen regional and local food systems. This article examines wholesale facility (hub) locations in food supply chain systems on a national scale to facilitate the efficient transfer of food from production regions to consumption locations. It designs an optimal national wholesale or hub location network to serve food consumption markets through efficient connections with production sites. The mathematical formulation is a mixed integer linear programming (MILP) problem that minimizes total network costs which include costs of transporting goods and locating facilities. A scenario study is used to examine the model's sensitivity to parameter changes, including travel distance, hub capacity, transportation cost, etc. An application is made to the U.S. fruit and vegetable industry. We demonstrate how parameter changes affect the optimal locations and number of wholesale facilities. Published by Elsevier B.V. C1 [Etemadnia, Hamideh; Goetz, Stephan J.] Penn State Univ, Dept Agr Econ Sociol & Educ, University Pk, PA 16802 USA. [Goetz, Stephan J.] Northeast Reg Ctr Rural Dev, University Pk, PA 16802 USA. [Canning, Patrick] Econ Res Serv, USDA, Washington, DC 20250 USA. [Tavallali, Mohammad Sadegh] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 119260, Singapore. RP Etemadnia, H (reprint author), Penn State Univ, Dept Agr Econ Sociol & Educ, University Pk, PA 16802 USA. EM hze1@psu.edu FU USDA NIFA grant [2011-68004-30057] FX Views presented here are those of the authors and do not necessarily reflect official policy of ERS, USDA, or Penn State University. Partial funding under USDA NIFA grant no. 2011-68004-30057 is gratefully acknowledged. NR 35 TC 7 Z9 7 U1 7 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-2217 EI 1872-6860 J9 EUR J OPER RES JI Eur. J. Oper. Res. PD JUL 16 PY 2015 VL 244 IS 2 BP 648 EP 661 DI 10.1016/j.ejor.2015.01.044 PG 14 WC Management; Operations Research & Management Science SC Business & Economics; Operations Research & Management Science GA CF1TR UT WOS:000352331300026 ER PT J AU Garrett, EF Po, E Bichi, ER Hexum, SK Melcher, R Hubner, AM AF Garrett, Edgar F. Po, Eleonora Bichi, Elena R. Hexum, Suzette K. Melcher, Robert Hubner, Andrew M. TI Clinical disease associated with epizootic hemorrhagic disease virus in cattle in Illinois SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Article ID WHITE-TAILED DEER; IBARAKI VIRUS; SEROTYPE 6; RESEMBLING BLUETONGUE; INFECTION; CALVES; ANTIBODIES; ORBIVIRUS; INDIANA; AGENT AB Objective-To describe the clinical manifestation of epizootic hemorrhagic disease virus (EHDV) in cattle during an outbreak in northwestern Illinois in the fall of 2013. Design-Outbreak survey. Sample-18 EHDV-affected cattle herds. Procedures-Herds with confirmed EHDV-infected cattle were selected for the survey on the basis of having a manager willing to participate. A survey was developed and administered to obtain information regarding the demographics of EHDV-affected herds and the clinical signs and outcomes for EHDV-infected cattle. Results-The managers of 13 beef and 5 dairy herds participated in the survey. The herds contained approximately 1,400 cattle, of which 61 were infected with EHDV and 16 died. Most cattle clinically affected with EHDV were adults, although 1 herd had 6 calves with clinical signs, and EHDV was identified during postmortem testing of 1 of those calves. Clinical signs most commonly observed were oral ulcerations or erosions, anorexia, weight loss, and lameness that typically lasted > 7 days. Of the 18 herds, 17 had wooded areas and 14 had at least 1 pond or marsh on the property. Deer were observed on the property of all farms. The EHDV outbreak in cattle coincided with an EHDV outbreak in deer in the region. Conclusions and Clinical Relevance-This large-scale outbreak of EHDV in US cattle was unusual because calves were affected. Because there is no way to effectively predict or prevent an EHDV outbreak in cattle, development of a vaccine for use in cattle during an outbreak would be beneficial. C1 [Garrett, Edgar F.; Po, Eleonora; Bichi, Elena R.] Univ Illinois, Coll Vet Med, Dept Vet Clin Med, Urbana, IL 61802 USA. [Hexum, Suzette K.] Vet Serv, Illinois Field Vet Med Off, APHIS, USDA, Springfield, IL 62711 USA. [Melcher, Robert] Stockton Blackhawk Vet Clin, Stockton, IL 61085 USA. [Hubner, Andrew M.] Lena Vet Clin, Lena, IL 61048 USA. RP Garrett, EF (reprint author), Univ Illinois, Coll Vet Med, Dept Vet Clin Med, Urbana, IL 61802 USA. EM efg@illinois.edu NR 29 TC 2 Z9 2 U1 1 U2 7 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 EI 1943-569X J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD JUL 15 PY 2015 VL 247 IS 2 BP 190 EP 195 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CP4KV UT WOS:000359851400026 PM 26133219 ER PT J AU Geng, P Harnly, JM Chen, P AF Geng, Ping Harnly, James M. Chen, Pei TI Differentiation of Whole Grain from Refined Wheat (T-aestivum) Flour Using Lipid Profile of Wheat Bran, Germ, and Endosperm with UHPLC-HRAM Mass Spectrometry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE whole grain; UHPLC; APCI; ESI; PCA; phospholipid; galactolipid; triglyceride; alk(en)ylresorcinols; gamma-oryzanol; sphingolipid ID LIQUID-CHROMATOGRAPHY; FATTY-ACIDS; OXIDATION-PRODUCTS; PDA-ESI/HRMSN; BREAD-MAKING; PHOSPHOLIPIDS; POLYPHENOLS; RYE; ALKYLRESORCINOLS; IDENTIFICATION AB A comprehensive analysis of wheat lipids from milling fractions of bran, germ, and endosperm was performed using ultrahigh-performance liquid chromatography high-resolution accurate-Mass multistage mass spectrometry (UHPLC-HRAM-MSn) with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) in both positive and negative modes. About 155 lipid compounds, including free fatty acids (FA), oxylipins, alk(en)ylresoreinols (ARs), gamma-oryzanol, sphingolipids, triglycerides (TGs), diglycerides (DGs), phospholipids, and galactolipids were characterized from the three milling fractions. Galactolipids and phospholipids were proposed to be potential discriminatory compounds for refined flour, whereas gamma-oryzanols, ARs, TGs, and DGs could distinguish whole wheat flour from a refined one based on principal component analysis (PCA). C1 [Geng, Ping; Harnly, James M.; Chen, Pei] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Chen, P (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Bldg 161,BARC E,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; National Institutes of Health [Y01 OD001298-01] FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health (Grant Y01 OD001298-01). NR 49 TC 2 Z9 2 U1 8 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 15 PY 2015 VL 63 IS 27 BP 6189 EP 6211 DI 10.1021/acs.jafc.5b01599 PG 23 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CN1NI UT WOS:000358186300011 PM 26083013 ER PT J AU Zhang, YX Chen, HL Maleki, SJ Cao, MJ Zhang, LJ Su, WJ Liu, GM AF Zhang, Yong-Xia Chen, Heng-Li Maleki, Soheila J. Cao, Min-Jie Zhang, Ling-Jing Su, Wen-Jin Liu, Guang-Ming TI Purification, Characterization, and Analysis of the Allergenic Properties of Myosin Light Chain in Procambarus clarkii SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Procambarus clarkii; myosin light chain; purification; physicochemical characterization; allergenicity; epitopes ID CALCIUM-BINDING PROTEIN; ARGININE KINASE; IMMUNOLOGICAL CHARACTERIZATION; SERINE PROTEINASE; SKELETAL-MUSCLE; SHRIMP ALLERGEN; CDNA CLONING; IDENTIFICATION; TROPOMYOSIN; STABILITY AB Myosin light chain (MLC) plays a vital role in cell and muscle functions and has been identified as an allergen in shrimp. In this study, MLC with a molecular mass of 18 kDa was purified from crayfish (Procambarus clarkii) muscle. Its physicochemical characterization showed that the purified MLC is a glycoprotein with 4.3% carbohydrate, highly stable to heat, acid-alkali, and digestion, and weakly retains IgE-binding activity when its secondary structure as altered. Serological assays suggested that conformational epitopes predominate over linear epitopes in the purified MLC. Two isoforms of the MLC gene (MLC1 and MLC2) were cloned, and the purified MLC was identified as MLC1. Analysis of the secondary and tertiary structures of the MLCs indicated that MLC1 has four conformational epitopes and three linear epitopes, whereas MLC2 had a major conformational epitope and three linear epitopes. These results are significant for understanding hypersensitization of humans to crayfish. C1 [Zhang, Yong-Xia; Chen, Heng-Li; Cao, Min-Jie; Zhang, Ling-Jing; Su, Wen-Jin; Liu, Guang-Ming] Jimei Univ, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Coll Food & Biol Engn, Xiamen 361021, Fujian, Peoples R China. [Maleki, Soheila J.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Liu, GM (reprint author), Jimei Univ, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Coll Food & Biol Engn, 43 Yindou Rd, Xiamen 361021, Fujian, Peoples R China. EM gmliu@jmu.edu.cn FU National Natural Scientific Foundation of China [31171660, U1405214]; Foundation for Innovative Research Team of Jimei University [2010A005] FX This work was supported by the Grant from the National Natural Scientific Foundation of China (31171660, U1405214) and the Foundation for Innovative Research Team of Jimei University (2010A005). NR 35 TC 2 Z9 2 U1 2 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 15 PY 2015 VL 63 IS 27 BP 6271 EP 6282 DI 10.1021/acs.jafc.5b01318 PG 12 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CN1NI UT WOS:000358186300018 PM 26083097 ER PT J AU Ashton, LM Hutchesson, MJ Rollo, ME Morgan, PJ Thompson, DI Collins, CE AF Ashton, Lee M. Hutchesson, Melinda J. Rollo, Megan E. Morgan, Philip J. Thompson, Debbe I. Collins, Clare E. TI Young adult males' motivators and perceived barriers towards eating healthily and being active: a qualitative study SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Young men; Focus groups; Qualitative research; Physical activity; Healthy eating; Obesity; Health behaviour ID RANDOMIZED CONTROLLED-TRIAL; BODY-MASS INDEX; WEIGHT-LOSS; PHYSICAL-ACTIVITY; UNIVERSITY-STUDENTS; FOCUS GROUPS; OBESE MEN; OVERWEIGHT; EXERCISE; COLLEGE AB Background: There is a lack of understanding of young men's perspectives in obesity-related research. This study aims to: (1) identify young men's perceived motivators and barriers in adopting healthy eating and physical activity behaviours, and (2) explore any differences in responses by weight status categories. Methods: Ten focus groups (32-63 minutes; 3-9 participants per group) were conducted with 61 young men (BMI: 25.3 +/- 5.1 kg/m(2), aged: 18-25 years) from the Hunter region, New South Wales, Australia. There were 35 (57.4 %) healthy weight men and 26 (42.6 %) overweight/obese men. Three groups were with healthy weight participants, three with overweight/obese participants and four with mixed-BMI participants. Sessions were audio-recorded and transcribed. Data analysis was conducted by an independent researcher using NVIVO10. Results: Motivators for healthy eating grouped into four themes: physical health (e.g. to live longer), sport or performance (e.g. to support their sporting goals), physical appearance (e.g. sexual attractiveness) and social influences (e.g. societal expectations to eat healthy), while key motivators for physical activity were: physical appearance (e.g. sexual attractiveness), social inclusion (e.g. making friends), physical and mental health (e.g. relieve stress) and improvements for sport or performance (e.g. improve fitness). Themes for key barriers to eating healthy were: intrinsic (e.g. perceived effort to adopt healthy eating), logistic (e.g. cost), and social factors (e.g. peer influence), while busy lifestyles (e.g. lack of time), logistic (e.g. cost), cognitive-emotional (e.g. feelings of inferiority) and social factors (e.g. family upbringing) were key barriers for physical activity. Responses varied little by BMI status. Conclusion: This research emphasises the importance of consulting young men when developing healthy lifestyle programs that aim to promote healthy eating and physical activity in young men. Future research is needed to identify the most effective ways to address their motivators and barriers in intervention research. C1 [Ashton, Lee M.; Hutchesson, Melinda J.; Rollo, Megan E.; Collins, Clare E.] Univ Newcastle, Prior Res Ctr Phys Act & Nutr, Fac Hlth & Med, Sch Hlth Sci, Callaghan, NSW 2308, Australia. [Morgan, Philip J.] Univ Newcastle, Prior Res Ctr Phys Act & Nutr, Fac Educ & Arts, Sch Educ, Callaghan, NSW 2308, Australia. [Thompson, Debbe I.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Collins, CE (reprint author), Univ Newcastle, Prior Res Ctr Phys Act & Nutr, Fac Hlth & Med, Sch Hlth Sci, Callaghan, NSW 2308, Australia. EM clare.collins@newcastle.edu.au FU 3D Healing Walk grant by Hunter Medical Research Institute (HMRI); International Postgraduate Award Scholarship; Greaves Family Medical Research Scholarship through HMRI; National Heart Foundation of Australia [100177] FX We would like to thank the independent qualitative researcher (Vibeke Hansen) for undertaking the data analysis and for the assistant moderator (Elroy Aguiar) who helped with time-keeping and note-taking for each focus group session. This research project was funded by a 3D Healing Walk grant provided by Hunter Medical Research Institute (HMRI). L.M. Ashton undertook this research as a part requirement for the degree of PhD (Nutrition and Dietetics), The University of Newcastle, Australia. L.M. Ashton is supported by an International Postgraduate Award Scholarship and The Greaves Family Medical Research Scholarship through HMRI. M.J Hutchesson is supported by a Postdoctoral Research Fellowship from the National Heart Foundation of Australia (#100177). NR 52 TC 8 Z9 8 U1 4 U2 46 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD JUL 15 PY 2015 VL 12 AR 93 DI 10.1186/s12966-015-0257-6 PG 10 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA CM7EQ UT WOS:000357853600001 PM 26169503 ER PT J AU Han, HS Oneil, E Bergman, RD Eastin, IL Johnson, LR AF Han, Han-Sup Oneil, Elaine Bergman, Richard D. Eastin, Ivan L. Johnson, Leonard R. TI Cradle-to-gate life cycle impacts of redwood forest resource harvesting in northern California SO JOURNAL OF CLEANER PRODUCTION LA English DT Article DE Redwood lumber; Life cycle analysis; Environmental impacts; Fuel consumption in timber harvesting; Air quality impacts ID ENVIRONMENTAL IMPACTS; WOOD PRODUCTS; DECAY RESISTANCE; INLAND NORTHWEST; PERFORMANCE; INVENTORY; HEARTWOOD; DECKING AB The first life cycle impact assessment for redwood (Sequoia sempervirens) forest management activities (i.e. a cradle-to-sawmill gate input) including the growing, harvesting, and hauling of redwood sawlogs to a sawmill was completed. In the stump-to-truck timber harvesting analysis, primary transport activities such as skidding and yarding consumed the largest amount of fuel and consequently generated the greatest environmental impacts (50% of the total) compared with the other harvesting stages (felling, processing, and loading). Hauling sawlogs to the sawmill was also a major contributor to total greenhouse gas emissions, representing 20% of total emissions. The most efficient harvest method in terms of fuel consumption per m(3) of wood harvested was the manual ground-based system used in even-aged silvicultural operations, followed by the skyline harvesting methods. Relative to even-aged silvicultural systems, uneven-aged systems, often prescribed to meet environmental goals such as maintaining biodiversity and protecting wildlife habitats, used 20% more fuel per m(3) of redwood sawlogs harvested. This was because uneven-aged systems required an increased number of entries to harvest the same amount of wood as even-aged systems. The cradle-to-gate GHG emissions from redwood forest management activities including growing, harvesting and hauling the logs represent only 14% (17.13 kg CO(2)e/m(3)) of the total GHG emissions (i.e. cradle-to-grave) associated with redwood decking lumber. The study results showed substantial differences in environmental impacts for the various harvesting operations and silvicultural systems for redwood forest management and operations. Therefore, the life cycle impact assessment results for the various redwood harvesting and silvicultural systems should be carefully considered when evaluating environmental performance of forest management activities along with other objectives. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Han, Han-Sup] Humboldt State Univ, Arcata, CA 95515 USA. [Oneil, Elaine; Eastin, Ivan L.] Univ Washington, Seattle, WA 98195 USA. [Bergman, Richard D.] USDA, Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Johnson, Leonard R.] Univ Idaho, Moscow, ID 83843 USA. RP Han, HS (reprint author), Humboldt State Univ, Arcata, CA 95515 USA. EM hh30@humboldt.edu; eoneil@uw.edu; rbergman@fs.fed.us; eastin@uw.edu; lrkmjohnson@frontier.com FU California Redwood Association FX We gratefully acknowledge the financial assistance for this research project provided by the California Redwood Association. NR 38 TC 2 Z9 2 U1 5 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0959-6526 EI 1879-1786 J9 J CLEAN PROD JI J. Clean Prod. PD JUL 15 PY 2015 VL 99 BP 217 EP 229 DI 10.1016/j.jclepro.2015.02.088 PG 13 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA CK4LJ UT WOS:000356195000018 ER PT J AU Cao, J Ou, GM Yang, NL Ding, KH Kream, BE Hamrick, MW Isales, CM Shi, XM AF Cao, Jay Ou, Guomin Yang, Nianlan Ding, Kehong Kream, Barbara E. Hamrick, Mark W. Isales, Carlos M. Shi, Xing-Ming TI Impact of targeted PPAR gamma disruption on bone remodeling SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Review DE PPAR gamma; MSC; Aging; Osteoblasts; Bone loss ID ACTIVATED-RECEPTOR-GAMMA; MESENCHYMAL STEM-CELLS; NECROSIS-FACTOR-ALPHA; MARROW STROMAL CELLS; LEUCINE-ZIPPER GILZ; ADIPOSE-TISSUE; OSTEOGENIC DIFFERENTIATION; OSTEOCLAST DIFFERENTIATION; MINERAL DENSITY; SERUM ADIPONECTIN AB Peroxisome proliferator-activated receptor gamma (PPAR gamma), known as the master regulator of adipogenesis, has been regarded as a promising target for new anti-osteoporosis therapy due to its role in regulating bone marrow mesenchymal stem/progenitor cell (BMSC) lineage commitment. However, the precise mechanism underlying PPAR gamma regulation of bone is not clear as a bone-specific PPAR gamma conditional knockout (cKO) study has not been conducted and evidence showed that deletion of PPAR gamma in other tissues also have profound effect on bone. In this study, we show that mice deficiency of PPAR gamma in cells expressing a 3.6 kb type I collagen promoter fragment (PPAR(fl/fl):Col3.6-Cre) exhibits a moderate, site-dependent bone mass phenotype. In vitro studies showed that adipogenesis is abolished completely and osteoblastogenesis increased significantly in both primary bone marrow culture and the BMSCs isolated from PPAR gamma cKO mice. Histology and histomorphometry studies revealed significant increases in the numbers of osteoblasts and surface in the PPAR gamma cKO mice. Finally, we found that neither the differentiation nor the function of osteoclasts was affected in the PPAR gamma cKO mice. Together, our studies indicate that PPAR gamma plays an important role in bone remodeling by increasing the abundance of osteoblasts for repair, but not during skeletal development. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Cao, Jay] USDA ARS Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Ou, Guomin; Yang, Nianlan; Ding, Kehong; Isales, Carlos M.; Shi, Xing-Ming] Georgia Regents Univ, Dept Neurosci & Regenerat Med, Augusta, GA 30912 USA. [Kream, Barbara E.] Univ Connecticut, Ctr Hlth, Dept Med, Farmington, CT USA. [Hamrick, Mark W.] Georgia Regents Univ, Dept Cell Biol, Augusta, GA 30912 USA. [Isales, Carlos M.; Shi, Xing-Ming] Georgia Regents Univ, Dept Orthopaed Surg, Augusta, GA 30912 USA. RP Shi, XM (reprint author), Georgia Regents Univ, Dept Neurosci & Regenerat Med, 1120 15th St, Augusta, GA 30912 USA. EM xshi@gru.edu RI Hamrick, Mark/K-1131-2016; OI Shi, Xing-Ming/0000-0003-4920-0989 FU National Institute On Aging of the National Institutes of Health [R01AG04 6248, P01AG036675, R01DK076045]; National Institute Of Diabetes And Digestive And Kidney Diseases; USDA Agricultural Research Service program [5450-51000-048-00D] FX We thank Dr. Frank J. Gonzalez, National Cancer Institute, NIH, for providing foxed PPAR gamma mouse. We thank Byung Lee for his involvement of this study. Research reported in this publication was supported by the National Institute On Aging of the National Institutes of Health under Award Numbers R01AG04 6248, P01AG036675, and R01DK076045 from the National Institute Of Diabetes And Digestive And Kidney Diseases. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Fund to J.C. was from the USDA Agricultural Research Service program "Food Factors to Prevent Obesity and Related Diseases", Current Research Information System no. 5450-51000-048-00D. NR 54 TC 6 Z9 6 U1 0 U2 6 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD JUL 15 PY 2015 VL 410 IS C SI SI BP 27 EP 34 DI 10.1016/j.mce.2015.01.045 PG 8 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA CK4QH UT WOS:000356207800005 PM 25666993 ER PT J AU Mask, TA Schoenecker, KA Kane, AJ Ransom, JI Bruemmer, JE AF Mask, Tracy A. Schoenecker, Kathryn A. Kane, Albert J. Ransom, Jason I. Bruemmer, Jason E. TI Serum antibody immunoreactivity to equine zona protein after SpayVac vaccination SO THERIOGENOLOGY LA English DT Article DE Contraception; Equus caballus; Fertility control; Immunocontraception; Porcine ZP ID WHITE-TAILED DEER; PELLUCIDA GLYCOPROTEINS; IMMUNOCONTRACEPTION; BIOSYNTHESIS; EXPRESSION; OOCYTES; HORSES AB Immunocontraception with porcine ZP (pZP) can be an effective means of fertility control in feral horses. Previous studies suggest that antibodies produced after pZP vaccination may both inhibit fertilization and cause follicular dysgenesis. Zonastat-H, PZP-22, and SpayVac are three pZP vaccines proposed for use in horses. Although all these vaccines contain the pZP antigen, variations in antigen preparation and vaccine formulation lead to differences in antigenic properties among them. Likewise, despite numerous efficacy and safety studies of Zonastat-H and PZP-22, the contraceptive mechanisms of SpayVac remain unclear. The preparation of pZP for SpayVac is thought to include more nonzona proteins, making it less pure than the other two vaccines. This may result in increased antigenicity of the vaccine. We therefore investigated the immunoreactivity of serum antibodies from SpayVac-vaccinated mares to equine zona protein. Western blot analyses revealed an immunoreactivity of these antibodies to protein isolated from mature equine oocytes, ZP, follicular tissues, and ovarian tissues. Immunohistochemical analyses were used to locate the binding of serum antibodies to the ZP of immature oocytes in ovarian stromal tissue. We also found serum antibodies from SpayVac-treated mares to be predominantly specific for zona protein 3. Collectively, our results suggest a model where serum antibodies produced in response to SpayVac vaccination are immunoreactive to equine zona protein in vitro. Our study lends insight into the contraceptive mechanisms underlying the infertility observed after SpayVac vaccination. (C) 2015 Elsevier Inc. All rights reserved. C1 [Mask, Tracy A.; Schoenecker, Kathryn A.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO USA. [Mask, Tracy A.; Bruemmer, Jason E.] Colorado State Univ, Anim Reprod & Biotechnol Lab, Ft Collins, CO 80523 USA. [Kane, Albert J.] US Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO USA. [Ransom, Jason I.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. RP Bruemmer, JE (reprint author), Colorado State Univ, Anim Reprod & Biotechnol Lab, Ft Collins, CO 80523 USA. EM Jason.Bruemmer@colostate.edu FU United States Geological Survey Wildlife Program FX This research was funded by the United States Geological Survey Wildlife Program and conducted through a cooperative effort between the USGS Fort Collins Science center, Bureau of Land Management, United States Department of Agriculture's Animal and Plant Health Inspection Service, and Colorado State University. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the United States Government or Colorado State University. NR 22 TC 1 Z9 2 U1 2 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X EI 1879-3231 J9 THERIOGENOLOGY JI Theriogenology PD JUL 15 PY 2015 VL 84 IS 2 BP 261 EP 267 DI 10.1016/j.theriogenology.2015.03.012 PG 7 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA CK0JH UT WOS:000355891000011 PM 25922172 ER PT J AU Gonzalez, P Battles, JJ Collins, BM Robards, T Saah, DS AF Gonzalez, Patrick Battles, John J. Collins, Brandon M. Robards, Timothy Saah, David S. TI Aboveground live carbon stock changes of California wildland ecosystems, 2001-2010 SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Carbon; Climate change; Protected areas; Uncertainty; Wildfire ID CLIMATE-CHANGE; FOREST; FIRE; US; BIOMASS; EMISSIONS; DISTURBANCE; GRASSLANDS; TEMPERATE; IMPACTS AB The balance between ecosystem emissions of carbon to the atmosphere and removals from the atmosphere indicates whether ecosystems are exacerbating or reducing climate change. Forest ecosystems in the State of California, USA, contain carbon that reaches the highest densities (mass per unit area) in the world, but it has been unresolved whether California ecosystems currently comprise a net sink or source of carbon. The California Global Warming Solutions Act of 2006 established greenhouse gas reduction targets for fossil fuel-burning sectors and ecosystems, underscoring the importance of tracking ecosystem carbon. Here, we conduct statewide spatial inventories of the aboveground live carbon stocks of forests and other terrestrial ecosystems of California, excluding agricultural and urban areas. We analyzed biomass data from field measurements of the Forest Inventory and Analysis program, published biomass information and remote sensing data on non-forest vegetation, and spatial distributions of vegetation types, height, and fractional cover derived by the Landfire program from Landsat remote sensing at 30 m spatial resolution. We conducted Monte Carlo analyses of the uncertainty of carbon stock change estimates from errors in tree biomass estimates, remote sensing, and estimates of the carbon fraction of biomass. The carbon stock in aboveground biomass was 850 +/- 230 Tg (mean +/- 95% confidence interval) in 2010. We found a net aboveground live carbon stock change of -69 +/- 15 Tg from 2001 to 2010, a rate of change of -0.8 +/- 0.2%y(-1). Due to slow decay of some dead wood, all of the live carbon stock change does not immediately generate emissions. Wildfires on 6% of the state analysis area produced two-thirds of the live carbon stock loss. This suggests that increased tree densities from a century of fire suppression have allowed the accumulation of fuel for carbon losses in recent wildfires. Remote sensing errors in vegetation classification accounted for most of the uncertainty in the carbon stock change estimates. Improvements are also needed to track spatial patterns of growth and dead wood. Our results establish the beginning of a time series for the state greenhouse gas inventory and provide information on the role of forest conservation and management in California in mitigating global climate change. Published by Elsevier B.V. C1 [Gonzalez, Patrick] Natl Pk Serv, Nat Resource Stewardship & Sci, Washington, DC 20005 USA. [Battles, John J.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Collins, Brandon M.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Robards, Timothy; Saah, David S.] Spatial Informat Grp, Pleasanton, CA 94588 USA. [Saah, David S.] Univ San Francisco, Dept Environm Sci, San Francisco, CA 94117 USA. RP Gonzalez, P (reprint author), Natl Pk Serv, Nat Resource Stewardship & Sci, Washington, DC 20005 USA. EM patrick_gonzalez@nps.gov RI Battles, John/G-8233-2012; Gonzalez, Patrick/B-9479-2013 OI Battles, John/0000-0001-7124-7893; Gonzalez, Patrick/0000-0002-7105-0561 FU California Air Resources Board (ARB) [10-778]; U.S. National Park Service Climate Change Response Program FX We gratefully acknowledge funding from the California Air Resources Board (ARB) under agreement 10-778, additional funding to Patrick Gonzalez from the U.S. National Park Service Climate Change Response Program, technical contributions from Klaus Scott, spatial data from Carlos Ramirez and the Forest Service Region 5 remote sensing laboratory, and supporting research by Debra Larson, John Sanders, Sheri Spiegal, and Natalie van Doom. We thank Richard Bode, Anny Huang, and Webster Tasat of ARB and Bruce Goines, Chris Keithley, Mark Rosenberg, David Stoms, and Mark Wenzel of the ARB Forest and Rangeland Greenhouse Gas Inventory Interagency Technical Working Group. NR 59 TC 8 Z9 9 U1 5 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2015 VL 348 BP 68 EP 77 DI 10.1016/j.foreco.2015.03.040 PG 10 WC Forestry SC Forestry GA CJ2ZN UT WOS:000355353300007 ER PT J AU Hanula, JL Horn, S O'Brien, JJ AF Hanula, James L. Horn, Scott O'Brien, Joseph J. TI Have changing forests conditions contributed to pollinator decline in the southeastern United States? SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Apoidea; Pollinator decline; Forest cover; Native bees; Solitary bees; Forest health ID RIPARIAN FORESTS; SIREX-NOCTILIO; OUACHITA MOUNTAINS; VISITING INSECTS; SPEYERIA-DIANA; NORTH-CAROLINA; NATIVE BEES; DIVERSITY; FIRE; PLANT AB Two conservation goals of the early 20th century, extensive reforestation and reduced wildfire through fire exclusion, may have contributed to declining pollinator abundance as forests became denser and shrub covered. To examine how forest structure affects bees we selected 5 stands in each of 7 forest types including: cleared forest; dense young pines; thinned young pines; mature open pine with extensive shrub/sapling cover; mature open pine with extensive herbaceous plant cover and little shrub cover; mature upland hardwood forest; and mature riparian hardwood forest. We sampled bees during the 2008 growing season using pan traps and measured overstory tree density, understory herbaceous plant and shrub diversity and cover, light penetration, and leaf area index. Numbers of bees and numbers of species per plot were highest in cleared forest and in mature pine stands with an herbaceous plant understory. Estimates of asymptotic species richness were highest in mature riparian hardwood forests, cleared forests and open pine forests with an herbaceous plant understory. Bee communities in the cleared forests and in the mature pine with an herbaceous plant understory were grouped together in ordination space which was consistent with perMANOVA results. The best predictor variable for bee species density was total tree basal area which was negatively correlated (r(2) = 0.58), while the best model for predicting bee abundance (r(2) = 0.62) included canopy openness, plant species density (both positively correlated) and shrub cover (negatively correlated). Our results combined with many others show that thinning forests combined with shrub control provides good bee habitat, is compatible with habitat restoration and management for other species, and the resulting forests will be healthier and less susceptible to old (e.g., southern pine beetle, Dendrocronus frontalis) and new (European woodwasp, Sirex noctilio) threats. Published by Elsevier B.V. C1 [Hanula, James L.; Horn, Scott; O'Brien, Joseph J.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Hanula, JL (reprint author), US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. EM jhanula@fs.fed.us FU USDA Forest Service, Southern Research Station's research work unit [4552] FX We thank Jason Gibbs for identifying Lasioglossum spp., Mike Cody and Chris Crowe for assistance in the field and laboratory, and John Nowak and Kay Kirkman for reviewing earlier drafts of this manuscript. Wenhua Pan, Kim Love-Myers, and Yuan Zhang provided assistance with statistical analyses. Funding for the study was provided by the USDA Forest Service, Southern Research Station's research work unit 4552, Insects, Diseases and Invasive Plants. NR 84 TC 5 Z9 6 U1 12 U2 87 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2015 VL 348 BP 142 EP 152 DI 10.1016/j.foreco.2015.03.044 PG 11 WC Forestry SC Forestry GA CJ2ZN UT WOS:000355353300014 ER PT J AU Vodde, F Jogiste, K Engelhart, J Frelich, LE Moser, WK Sims, A Metslaid, M AF Vodde, Floor Jogiste, Kalev Engelhart, Jeroen Frelich, Lee E. Moser, W. Keith Sims, Allan Metslaid, Marek TI Impact of wind-induced microsites and disturbance severity on tree regeneration patterns: Results from the first post-storm decade SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Mortality; Competition; Establishment sites; Regeneration dynamics; Windthrow ID SOUTHERN BOREAL FOREST; NORWAY SPRUCE; ADVANCE REGENERATION; PICEA-ABIES; SPECIES COEXISTENCE; SEEDLING MORTALITY; PLANT-COMMUNITIES; FAGUS-SYLVATICA; RAIN-FOREST; DEAD WOOD AB In two hemiboreal mixed spruce hardwood forests in north-east Estonia, we studied (1) which factors affect tree regeneration survival and development during the first post-storm decade and (2) how these effects change in time. Regeneration height and mortality of the tree species black alder (Alnus glutinosa (L.) J. Gaertn.), birch (Betula pendula Roth., Betula pubescens Ehrh.), Norway spruce (Picea abies (L.) Karst.) and European rowan (Sorbus aucuparia L.) were analysed in moderately and heavily damaged stands, in two types of windstorm-created microsites, i.e. root-plate pits and mounds of uprooted trees, and on intact soil at different stages since disturbance. Regeneration was significantly taller in heavily damaged areas and species traits regarding tree height only became noteworthy at later stages since disturbance. Mortality probability was initially indifferent to microsite type and increased later for regeneration on intact soil compared to regeneration on the storm-induced microsites. Mortality increased with storm severity for A. glutinosa and Betula, whereas mortality of P. abies was initially low and became higher with time since disturbance in areas with increased levels of coarse woody debris. Eventually, height and height increment in previous years were clearly negatively related to mortality probability and competition levels in previous years increased chance of death. The relatively high spatial heterogeneity and trends in dominance of post-storm microsites by different tree species increase disturbance-emulating management options. In conclusion, regeneration mortality and species composition are initially directed by exogenous factors linked to storm severity and microsite heterogeneity, generating a degree of spatial partitioning within a microsite, whereas gradually species' life-history traits and competition take over. (C) 2015 Elsevier B.V. All rights reserved. C1 [Vodde, Floor; Jogiste, Kalev; Engelhart, Jeroen; Metslaid, Marek] Estonian Univ Life Sci, Dept Forest Biol, EE-51014 Tartu, Estonia. [Sims, Allan] Estonian Univ Life Sci, Dept Forest Management, EE-51014 Tartu, Estonia. [Frelich, Lee E.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Moser, W. Keith] USDA, No Res Stn, Forest Serv, Forest Inventory & Anal Unit, St Paul, MN USA. RP Vodde, F (reprint author), Estonian Univ Life Sci, Dept Forest Biol, Kreutzwaldi 5, EE-51014 Tartu, Estonia. EM floorvodde@hotmail.com; kalev.jogiste@emu.ee; jeroenengelhart@gmx.com; freli001@umn.edu; moserk@safnet.org; allan.sims@emu.ee; marek.metslaid@emu.ee RI Sims, Allan/A-5119-2009; Vodde, Floortje/E-9465-2017; OI Sims, Allan/0000-0003-1312-6940; Vodde, Floortje/0000-0003-1764-6193; Frelich, Lee/0000-0002-9052-7070 FU Estonian Ministry of Education and Research [IUT21-4]; Environmental Investment Centre; Estonian Science Foundation [8496]; Estonian Research Council PUT [PUT715]; Prins Bernhard Cultuurfonds; Archimedes Foundation; [SF0170014s08] FX The authors wish to thank the fieldwork teams throughout the years, and the current and former forest districts for permission to carry out the field study. This study was supported by Grant No. SF0170014s08 and the Institutional Research Funding IUT21-4 from the Estonian Ministry of Education and Research, by the Environmental Investment Centre, the Estonian Science Foundation (Grant No. 8496), the Estonian Research Council grant PUT (PUT715), Prins Bernhard Cultuurfonds and the Archimedes Foundation. NR 58 TC 1 Z9 1 U1 5 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2015 VL 348 BP 174 EP 185 DI 10.1016/j.foreco.2015.03.052 PG 12 WC Forestry SC Forestry GA CJ2ZN UT WOS:000355353300017 ER PT J AU Thomazini, A Mendonca, S Teixeira, DB Almeida, ICC La Scala, N Canellas, LP Spokas, KA Milori, DMBP Turbay, CVG Fernandes, RBA Schaefer, CEGR AF Thomazini, A. Mendonca, S. Teixeira, D. B. Almeida, I. C. C. La Scala, N., Jr. Canellas, L. P. Spokas, K. A. Milori, D. M. B. P. Turbay, C. V. G. Fernandes, R. B. A. Schaefer, C. E. G. R. TI CO2 and N2O emissions in a soil chronosequence at a glacier retreat zone in Maritime Antarctica SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Greenhouse gases; Soil properties; Climate change; Carbon sink ID KING GEORGE ISLAND; ORGANIC-CARBON; MICROBIAL BIOMASS; CRYOSOLS; TEMPERATURE; NITROGEN; MATTER; FLUXES; TUNDRA AB Studies of C cycle alterations are extremely important to identify changes due to climate change, especially in the polar ecosystem. The objectives of this study were to (i) examine patterns of soil CO2-C and N2O-Nemissions, and (ii) evaluate the quantity and quality of soil organic matter across a glacier retreat chronosequence in the Maritime Antarctica. Field measurements were carried out during January and February 2010 (summer season) along a retreating zone of the White Eagle Glacier, at King George Island, Maritime Antarctica. Soil samples (0-10 cm) were collected along a 500-m transect at regular intervals to determine changes in soil organic matter. Field CO2-C emission measurements and soil temperature were carried out at regular intervals. In addition, greenhouse gas production potentials were assessed through 100 days laboratory incubations. Soils exposed for a longer time tended to have greater concentrations of soluble salts and possess sandier textures. Total organic C (3.59 g kg(-1)), total N (2.31 g kg(-1)) and labile C (1.83 g kg(-1)) tended to be lower near the glacier front compared with sites away from it, which is correlated with decreasing degree of humification of the soil organic matter with exposure time. Soil CO2-C emissions tended to increase with distance from the glacier front. On average, the presence of vegetation increased CO2-C emissions by 440%, or the equivalent of 0.633 g of CO2-C m(-2) h(-1). Results suggest that newly exposed landsurfaces undergo soil formation with increasing labile C input from vegetation, accompanied by increasing soil CO2-C emissions. Despite the importance of exposure time on CO2-C production and emissions, there was no similar trend in soil N2O-N production potentials as a function of glacial retreat. For N2O, instead, the maximum production occurred in sites with the first stages of vegetation growth. (C) 2015 Elsevier B.V. All rights reserved. C1 [Thomazini, A.; Mendonca, S.] Univ Fed Espirito Santo, Dept Plant Prod, BR-29500000 Alegre, Espirito Santo, Brazil. [Teixeira, D. B.; La Scala, N., Jr.] UNESP, FCAV, BR-14884900 Jaboticabal, SP, Brazil. [Almeida, I. C. C.] Inst Fed Norte Minas Gerais, Januaria, MG, Brazil. [Canellas, L. P.] Univ Estadual Norte Fluminense, Nucl Desenvolvimento Insumos Biol Agr NUDIBA, BR-28013602 Rio De Janeiro, Brazil. [Spokas, K. A.] Univ Minnesota, USDA ARS, Soil & Water Management Unit, St Paul, MN 55108 USA. [Milori, D. M. B. P.] Embrapa Instrumentat Brazilian Agr Res Corp, Sao Carlos, SP, Brazil. [Turbay, C. V. G.] Univ Fed Espirito Santo, Dept Geol, BR-29500000 Alegre, Espirito Santo, Brazil. [Fernandes, R. B. A.; Schaefer, C. E. G. R.] Univ Fed Vicosa, Dept Solos, BR-36570000 Vicosa, MG, Brazil. RP Thomazini, A (reprint author), Univ Fed Espirito Santo, Dept Plant Prod, BR-29500000 Alegre, Espirito Santo, Brazil. EM andre.thz@gmail.com; eduardo.mendonca@ufes.br; daniel.dbt@hotmail.com; ivancarreiro@yahoo.com.br; lascala@fcav.unesp.br; lucianocanellas@gmail.com; kurt.Spokas@ars.usda.gov; debora.milori@embrapa.br; cturbay@gmail.com; raphael@ufv.br; carlos.schaefer@ufv.br RI Milori, Debora/I-9861-2014; La Scala Jr., Newton/C-4398-2012; Milori, Debora/D-3875-2011; USP, CePOF/J-3608-2015; Teixeira, Daniel/O-4239-2014 OI Teixeira, Daniel/0000-0001-8172-7911 FU CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) [556794/2009-5]; MCTI (Ministerio da Ciencia, Tecnologia e Inovacao) FX We acknowledge CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) (556794/2009-5) and MCTI (Ministerio da Ciencia, Tecnologia e Inovacao) for granting financial support. We also acknowledge the United States Department of Agriculture-Agriculture Research Service and the University of Minnesota - St. Paul, MN USA for providing technical support. This work is a contribution of INCT-Criosfera TERRANTAR group. NR 48 TC 3 Z9 3 U1 3 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 15 PY 2015 VL 521 BP 336 EP 345 DI 10.1016/j.scitotenv.2015.03.110 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CH3ED UT WOS:000353909000036 PM 25855094 ER PT J AU Tillman, PG Cottrell, TE AF Tillman, P. Glynn Cottrell, Ted E. TI Spatiotemporal Distribution of Chinavia hilaris (Hemiptera: Pentatomidae) in Peanut-Cotton Farmscapes SO JOURNAL OF INSECT SCIENCE LA English DT Article DE inverse distance weighting; SADIE methodology; spatial distribution; noncrop host plant ID STINK BUGS HETEROPTERA; NEZARA-VIRIDULA HEMIPTERA; SEASONAL ABUNDANCE; GEORGIA FARMSCAPES; EUSCHISTUS-SERVUS; SOUTH-CAROLINA; PECAN ORCHARDS; TRAP CAPTURE; FIELD EDGES; DISPERSAL AB The green stink bug, Chinavia hilaris (Say) (Hemiptera: Pentatomidae), is a pest of cotton in the southeastern United States, but little is known concerning its spatiotemporal distribution in agricultural farmscapes. Therefore, spatiotemporal distribution of C. hilaris in farmscapes where cotton fields adjoined peanut was examined weekly. Spatial patterns of C. hilaris counts were analyzed using SADIE (Spatial Analysis by Distance Indices) methodology. Interpolated maps of C. hilaris density were used to visualize abundance and distribution of C. hilaris in crops. For the six peanut-cotton farmscapes studied, the frequency of C. hilaris in cotton (94.8%) was significantly higher than in peanut (5.2%), and nymphs were rarely detected in peanut, indicating that peanut was not a source of C. hilaris into cotton. Significantly, aggregated spatial distributions were detected in cotton. Maps of local clustering indices depicted patches of C. hilaris in cotton, mainly at field edges including the peanut-to-cotton interface. Black cherry (Prunus serotina Ehrh.) and elderberry (Sambucus nigra subsp. canadensis [L.] R. Bolli) grew in habitats adjacent to crops, C. hilaris were captured in pheromonebaited stink bug traps in these habitats, and in most instances, C. hilaris were observed feeding on black cherry and elderberry in these habitats before colonization of cotton. Spatial distribution of C. hilaris in these farmscapes revealed that C. hilaris colonized cotton field edges near these two noncrop hosts. Altogether, these findings suggest that black cherry and elderberry were sources of C. hilaris into cotton. Factors affecting the spatiotemporal dynamics of C. hilaris in peanut-cotton farmscapes are discussed. C1 [Tillman, P. Glynn] USDA ARS, Crop Protect & Management Res Lab, Tifton, GA 31793 USA. [Cottrell, Ted E.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Tillman, PG (reprint author), USDA ARS, Crop Protect & Management Res Lab, 2747 Davis Rd, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 40 TC 4 Z9 4 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUL 14 PY 2015 VL 15 AR 101 DI 10.1093/jisesa/iev081 PG 11 WC Entomology SC Entomology GA CN7LK UT WOS:000358615800004 ER PT J AU Skinner, DZ AF Skinner, Daniel Z. TI Genes Upregulated in Winter Wheat (Triticum aestivum L.) during Mild Freezing and Subsequent Thawing Suggest Sequential Activation of Multiple Response Mechanisms SO PLOS ONE LA English DT Article ID QUANTITATIVE EXPRESSION ANALYSIS; PLANT COLD-ACCLIMATION; LOW-TEMPERATURE; PHENYLPROPANOID PATHWAY; SPRING WHEAT; TOLERANCE; GLYCOSYLTRANSFERASES; SALINITY; ONTOLOGY; EXPOSURE AB Exposing fully cold-acclimated wheat plants to a mild freeze-thaw cycle of -3 degrees C for 24h followed by +3 degrees C for 24 or 48h results in dramatically improved tolerance of subsequent exposure to sub-freezing temperatures. Gene enrichment analysis of crown tissue from plants collected before or after the -3 degrees C freeze or after thawing at +3 degrees C for 24 or 48h revealed that many biological processes and molecular functions were activated during the freeze-thaw cycle in an increasing cascade of responses such that over 150 processes or functions were significantly enhanced by the end of the 48 h, post-freeze thaw. Nearly 2,000 individual genes were upregulated more than 2-fold over the 72 h course of freezing and thawing, but more than 70% of these genes were upregulated during only one of the time periods examined, suggesting a series of genes and gene functions were involved in activation of the processes that led to enhanced freezing tolerance. This series of functions appeared to include extensive cell signaling, activation of stress response mechanisms and the phenylpropanoid biosynthetic pathway, extensive modification of secondary metabolites, and physical restructuring of cell membranes. By identifying plant lines that are especially able to activate these multiple mechanisms it may be possible to develop lines with enhanced winterhardiness. C1 [Skinner, Daniel Z.] USDA ARS, Pullman, WA 99164 USA. [Skinner, Daniel Z.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Skinner, DZ (reprint author), USDA ARS, 209 Johnson Hall, Pullman, WA 99164 USA. EM dan.skinner@ars.usda.gov FU US Department of Agriculture intramural project [5348-21000-030-00D] FX This research was supported by US Department of Agriculture intramural project number 5348-21000-030-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 2 Z9 2 U1 0 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 14 PY 2015 VL 10 IS 7 AR e0133166 DI 10.1371/journal.pone.0133166 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1QN UT WOS:000358194900143 PM 26173115 ER PT J AU Wiedmann, RT Nonneman, DJ Rohrer, GA AF Wiedmann, Ralph T. Nonneman, Dan J. Rohrer, Gary A. TI Genome-Wide Copy Number Variations Using SNP Genotyping in a Mixed Breed Swine Population SO PLOS ONE LA English DT Article ID SINGLE NUCLEOTIDE POLYMORPHISMS; DOMESTIC PIG; VARIANTS; IDENTIFICATION; EXPRESSION; DISEASE AB Copy number variations (CNVs) are increasingly understood to affect phenotypic variation. This study uses SNP genotyping of trios of mixed breed swine to add to the catalog of known genotypic variation in an important agricultural animal. PorcineSNP60 BeadChip genotypes were collected from 1802 pigs that combined to form 1621 trios. These trios were from the crosses of 50 boars with 525 sows producing 1621 piglets. The pigs were part of a population that was a mix of 1/4 Duroc, 1/2 Landrace and 1/4 Yorkshire breeds. Merging the overlapping CNVs that were observed in two or more individuals to form CNV regions (CNVRs) yielded 502 CNVRs across the autosomes. The CNVRs intersected genes, as defined by RefSeq, 84% of the time - 420 out of 502. The results of this study are compared and contrasted to other swine studies using similar and different methods of detecting CNVR. While progress is being made in this field, more work needs to be done to improve consistency and confidence in CNVR results. C1 [Wiedmann, Ralph T.; Nonneman, Dan J.; Rohrer, Gary A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Rohrer, GA (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM gary.rohrer@ars.usda.gov FU Agricultural Research Service, a division of the US Department of Agriculture [5438-31000-083-00D] FX This work was funded by Current Research Information System #5438-31000-083-00D of the Agricultural Research Service, a division of the US Department of Agriculture. The funder did not contribute to the study design, data collation and analysis, decision to publish, or preparation of the manuscript. NR 33 TC 2 Z9 2 U1 0 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 14 PY 2015 VL 10 IS 7 AR e0133529 DI 10.1371/journal.pone.0133529 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1QN UT WOS:000358194900151 PM 26172260 ER PT J AU VanRaden, PM Sun, CY O'Connell, JR AF VanRaden, Paul M. Sun, Chuanyu O'Connell, Jeffrey R. TI Fast imputation using medium or low-coverage sequence data SO BMC GENETICS LA English DT Article DE Imputation; Genotype; Sequence read depth; Allele probability ID GENOME-WIDE ASSOCIATION; GENOTYPE IMPUTATION; LIVESTOCK POPULATIONS; DESIGN; CATTLE; PREDICTIONS; INFERENCE; SELECTION; ACCURACY; ANIMALS AB Background: Accurate genotype imputation can greatly reduce costs and increase benefits by combining whole-genome sequence data of varying read depth and array genotypes of varying densities. For large populations, an efficient strategy chooses the two haplotypes most likely to form each genotype and updates posterior allele probabilities from prior probabilities within those two haplotypes as each individual's sequence is processed. Directly using allele read counts can improve imputation accuracy and reduce computation compared with calling or computing genotype probabilities first and then imputing. Results: A new algorithm was implemented in findhap (version 4) software and tested using simulated bovine and actual human sequence data with different combinations of reference population size, sequence read depth and error rate. Read depths of >= 8x may be desired for direct investigation of sequenced individuals, but for a given total cost, sequencing more individuals at read depths of 2x to 4x gave more accurate imputation from array genotypes. Imputation accuracy improved further if reference individuals had both low-coverage sequence and high-density (HD) microarray data, and remained high even with a read error rate of 16 %. With read depths of <= 4x, findhap (version 4) had higher accuracy than Beagle (version 4); computing time was up to 400 times faster with findhap than with Beagle. For 10,000 sequenced individuals plus 250 with HD array genotypes to test imputation, findhap used 7 hours, 10 processors and 50 GB of memory for 1 million loci on one chromosome. Computing times increased in proportion to population size but less than proportional to number of variants. Conclusions: Simultaneous genotype calling from low-coverage sequence data and imputation from array genotypes of various densities is done very efficiently within findhap by updating allele probabilities within the two haplotypes for each individual. Accuracy of genotype calling and imputation were high with both simulated bovine and actual human genomes reduced to low-coverage sequence and HD microarray data. More efficient imputation allows geneticists to locate and test effects of more DNA variants from more individuals and to include those in future prediction and selection. C1 [VanRaden, Paul M.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Sun, Chuanyu] Natl Assoc Anim Breeders, Columbia, MO 65205 USA. [O'Connell, Jeffrey R.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. RP VanRaden, PM (reprint author), ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM Paul.VanRaden@ars.usda.gov FU Agricultural Research Service, United States Department of Agriculture (ARS-USDA); National Association of Animal Breeders; University of Maryland School of Medicine; ARS-USDA FX We thank Suzanne Hubbard for assistance with technical editing. The figures were created using Daniel's XL Toolbox addin for Excel (version 6.53) by Daniel Kraus, Wurzburg, Germany. PMV was funded solely by the Agricultural Research Service, United States Department of Agriculture (ARS-USDA). CS was funded solely by the National Association of Animal Breeders. JRO was funded by the University of Maryland School of Medicine and by ARS-USDA. The authors designed the study, analyzed the data, interpreted the results, and wrote and submitted the manuscript with no direct input from the funding sources. NR 37 TC 3 Z9 3 U1 4 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUL 14 PY 2015 VL 16 AR 82 DI 10.1186/s12863-015-0243-7 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA CM7EG UT WOS:000357852600003 PM 26168789 ER PT J AU Ort, DR Merchant, SS Alric, J Barkan, A Blankenship, RE Bock, R Croce, R Hanson, MR Hibberd, JM Long, SP Moore, TA Moroney, J Niyogi, KK Parry, MAJ Peralta-Yahya, PP Prince, RC Redding, KE Spalding, MH van Wijk, KJ Vermaas, WFJ von Caemmerer, S Weber, APM Yeates, TO Yuan, JS Zhu, XG AF Ort, Donald R. Merchant, Sabeeha S. Alric, Jean Barkan, Alice Blankenship, Robert E. Bock, Ralph Croce, Roberta Hanson, Maureen R. Hibberd, Julian M. Long, Stephen P. Moore, Thomas A. Moroney, James Niyogi, Krishna K. Parry, Martin A. J. Peralta-Yahya, Pamela P. Prince, Roger C. Redding, Kevin E. Spalding, Martin H. van Wijk, Klaas J. Vermaas, Wim F. J. von Caemmerer, Susanne Weber, Andreas P. M. Yeates, Todd O. Yuan, Joshua S. Zhu, Xin Guang TI Redesigning photosynthesis to sustainably meet global food and bioenergy demand SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE light capture/conversion; carbon capture/conversion; smart canopy; enabling plant biotechnology tools; sustainable crop production ID CO2 DIFFUSION; PHOTORESPIRATORY BYPASSES; CANOPY PHOTOSYNTHESIS; C-4 PHOTOSYNTHESIS; SYSTEMS BIOLOGY; ANTENNA SIZE; EFFICIENCY; RUBISCO; PRODUCTIVITY; INCREASE AB The world's crop productivity is stagnating whereas population growth, rising affluence, and mandates for biofuels put increasing demands on agriculture. Meanwhile, demand for increasing cropland competes with equally crucial global sustainability and environmental protection needs. Addressing this looming agricultural crisis will be one of our greatest scientific challenges in the coming decades, and success will require substantial improvements at many levels. We assert that increasing the efficiency and productivity of photosynthesis in crop plants will be essential if this grand challenge is to be met. Here, we explore an array of prospective redesigns of plant systems at various scales, all aimed at increasing crop yields through improved photosynthetic efficiency and performance. Prospects range from straightforward alterations, already supported by preliminary evidence of feasibility, to substantial redesigns that are currently only conceptual, but that may be enabled by new developments in synthetic biology. Although some proposed redesigns are certain to face obstacles that will require alternate routes, the efforts should lead to new discoveries and technical advances with important impacts on the global problem of crop productivity and bioenergy production. C1 [Ort, Donald R.] Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Ort, Donald R.; Long, Stephen P.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.; Long, Stephen P.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Merchant, Sabeeha S.; Yeates, Todd O.] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA. [Merchant, Sabeeha S.; Yeates, Todd O.] Univ Calif Los Angeles, Dept Energy, Inst Genom & Prote, Los Angeles, CA 90095 USA. [Alric, Jean] CNRS, Unite Mixte Rech Biol Vegetale & Microbiol Enviro, F-13115 St Paul Les Durance, France. [Barkan, Alice] Univ Oregon, Dept Biol, Eugene, OR 97403 USA. [Blankenship, Robert E.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Blankenship, Robert E.] Washington Univ, Dept Chem, St Louis, MO 63130 USA. [Bock, Ralph] Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany. [Croce, Roberta] Vrije Univ Amsterdam, Dept Phys & Astron, NL-1081 Amsterdam, Netherlands. [Hanson, Maureen R.] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA. [Hibberd, Julian M.] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England. [Long, Stephen P.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Moore, Thomas A.; Redding, Kevin E.] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA. [Moore, Thomas A.; Redding, Kevin E.; Vermaas, Wim F. J.] Arizona State Univ, Ctr Bioenergy & Photosynth, Tempe, AZ 85287 USA. [Moroney, James] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Niyogi, Krishna K.] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA. [Niyogi, Krishna K.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Niyogi, Krishna K.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA. [Parry, Martin A. J.] Rothamsted Res, Harpenden AL5 2JQ, Herts, England. [Peralta-Yahya, Pamela P.] Georgia Inst Technol, Dept Chem & Biochem, Atlanta, GA 30332 USA. [Prince, Roger C.] ExxonMobil Biomed Sci, Annandale, NJ 08801 USA. [Spalding, Martin H.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [van Wijk, Klaas J.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Vermaas, Wim F. J.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [von Caemmerer, Susanne] Australian Natl Univ, Res Sch Biol, Canberra, ACT 2601, Australia. [Weber, Andreas P. M.] Univ Dusseldorf, Dept Plant Biochem, D-40225 Dusseldorf, Germany. [Weber, Andreas P. M.] Univ Dusseldorf, Cluster Excellence Plant Sci, D-40225 Dusseldorf, Germany. [Yuan, Joshua S.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Zhu, Xin Guang] Chinese Acad Sci, CAS Key Lab Computat Biol, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China. RP Ort, DR (reprint author), Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM d-ort@illinois.edu RI Weber, Andreas/A-6250-2011; croce, roberta/N-4067-2014; von Caemmerer, Susanne/C-9317-2009; Alric, Jean/E-3538-2013 OI Hanson, Maureen/0000-0001-8141-3058; Parry, Martin/0000-0002-4477-672X; Yeates, Todd/0000-0001-5709-9839; Long, Stephen/0000-0002-8501-7164; Weber, Andreas/0000-0003-0970-4672; croce, roberta/0000-0003-3469-834X; Alric, Jean/0000-0003-3574-2234 FU Bill and Melinda Gates Foundation at the University of Illinois; Cold Spring Harbor Laboratory Corporate Sponsor Program FX We thank Haley Ahlers, who works on the Realizing Improved Photosynthetic Efficiency (RIPE) project funded by the Bill and Melinda Gates Foundation at the University of Illinois, for assistance with graphics. This paper was conceived at the workshop "Redesigning Photosynthesis-Identifying Opportunities and Novel Ideas" held at the Banbury Center, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, May 13-16, 2013. We thank the Cold Spring Harbor Laboratory Banbury Center for hosting and the Cold Spring Harbor Laboratory Corporate Sponsor Program for funding the workshop. NR 63 TC 59 Z9 63 U1 53 U2 175 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUL 14 PY 2015 VL 112 IS 28 BP 8529 EP 8536 DI 10.1073/pnas.1424031112 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CM7MU UT WOS:000357878700028 PM 26124102 ER PT J AU Dumpala, PR Peterson, BC Lawrence, ML Karsi, A AF Dumpala, Pradeep R. Peterson, Brian C. Lawrence, Mark L. Karsi, Attila TI Identification of Differentially Abundant Proteins of Edwardsiella ictaluri during Iron Restriction SO PLOS ONE LA English DT Article ID OUTER-MEMBRANE PROTEINS; ESCHERICHIA-COLI; CHANNEL CATFISH; PSEUDOMONAS-AERUGINOSA; VIBRIO-CHOLERAE; SMALL RNA; EXTRACELLULAR PRODUCTS; PUNCTATUS RAFINESQUE; PATHOGENIC BACTERIA; ENTERIC SEPTICEMIA AB Edwardsiella ictaluri is a Gram-negative facultative anaerobe intracellular bacterium that causes enteric septicemia in channel catfish. Iron is an essential inorganic nutrient of bacteria and is crucial for bacterial invasion. Reduced availability of iron by the host may cause significant stress for bacterial pathogens and is considered a signal that leads to significant alteration in virulence gene expression. However, the precise effect of iron-restriction on E. ictaluri protein abundance is unknown. The purpose of this study was to identify differentially abundant proteins of E. ictaluri during in vitro iron-restricted conditions. We applied two-dimensional difference in gel electrophoresis (2D-DIGE) for determining differentially abundant proteins and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS) for protein identification. Gene ontology and pathway-based functional modeling of differentially abundant proteins was also conducted. A total of 50 unique differentially abundant proteins at a minimum of 2-fold (p <= 0.05) difference in abundance due to iron-restriction were detected. The numbers of up-and down-regulated proteins were 37 and 13, respectively. We noted several proteins, including EsrB, LamB, MalM, MalE, FdaA, and TonB-dependent heme/hemoglobin receptor family proteins responded to iron restriction in E. ictaluri. C1 [Dumpala, Pradeep R.] Rogosin Inst, Xenia Div, Xenia, OH USA. [Peterson, Brian C.] USDA ARS, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. [Lawrence, Mark L.; Karsi, Attila] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. RP Karsi, A (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM a.karsi@msstate.edu FU United States Department of Agriculture; College of Veterinary Medicine FX The authors thank the United States Department of Agriculture and College of Veterinary Medicine for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 81 TC 2 Z9 2 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 13 PY 2015 VL 10 IS 7 AR e0132504 DI 10.1371/journal.pone.0132504 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1PW UT WOS:000358193100047 PM 26168192 ER PT J AU Hunter, MO Keller, M Morton, D Cook, B Lefsky, M Ducey, M Saleska, S de Oliveira, RC Schietti, J AF Hunter, Maria O. Keller, Michael Morton, Douglas Cook, Bruce Lefsky, Michael Ducey, Mark Saleska, Scott de Oliveira, Raimundo Cosme, Jr. Schietti, Juliana TI Structural Dynamics of Tropical Moist Forest Gaps SO PLOS ONE LA English DT Article ID RAIN-FOREST; CANOPY GAPS; PHASE REGENERATION; CENTRAL AMAZONIA; TREE DIVERSITY; BRAZILIAN AMAZON; STEADY-STATE; FIRME FOREST; IVORY-COAST; LIDAR DATA AB Gap phase dynamics are the dominant mode of forest turnover in tropical forests. However, gap processes are infrequently studied at the landscape scale. Airborne lidar data offer detailed information on three-dimensional forest structure, providing a means to characterize fine-scale (1 m) processes in tropical forests over large areas. Lidar-based estimates of forest structure (top down) differ from traditional field measurements (bottom up), and necessitate clear-cut definitions unencumbered by the wisdom of a field observer. We offer a new definition of a forest gap that is driven by forest dynamics and consistent with precise ranging measurements from airborne lidar data and tall, multi-layered tropical forest structure. We used 1000 ha of multi-temporal lidar data (2008, 2012) at two sites, the Tapajos National Forest and Ducke Reserve, to study gap dynamics in the Brazilian Amazon. Here, we identified dynamic gaps as contiguous areas of significant growth, that correspond to areas > 10 m(2), with height < 10 m. Applying the dynamic definition at both sites, we found over twice as much area in gap at Tapajos National Forest (4.8 %) as compared to Ducke Reserve (2.0 %). On average, gaps were smaller at Ducke Reserve and closed slightly more rapidly, with estimated height gains of 1.2 m y(-1) versus 1.1 m y(-1) at Tapajos. At the Tapajos site, height growth in gap centers was greater than the average height gain in gaps (1.3 m y(-1) versus 1.1 m y(-1)). Rates of height growth between lidar acquisitions reflect the interplay between gap edge mortality, horizontal ingrowth and gap size at the two sites. We estimated that approximately 10% of gap area closed via horizontal ingrowth at Ducke Reserve as opposed to 6 % at Tapajos National Forest. Height loss (interpreted as repeat damage and/or mortality) and horizontal ingrowth accounted for similar proportions of gap area at Ducke Reserve (13% and 10 %, respectively). At Tapajos, height loss had a much stronger signal (23 % versus 6 %) within gaps. Both sites demonstrate limited gap contagiousness defined by an increase in the likelihood of mortality in the immediate vicinity (similar to 6 m) of existing gaps. C1 [Hunter, Maria O.] Univ New Hampshire, Earth Sci Res Ctr, Durham, NH 03824 USA. [Keller, Michael] USDA Forest Serv, Int Inst Trop Forestry, San Juan, PR USA. [Keller, Michael] EMBRAPA Monitoramento Satelite, Campinas, SP, Brazil. [Morton, Douglas; Cook, Bruce] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Lefsky, Michael] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Ducey, Mark] Univ New Hampshire, Dept Nat Resources, Durham, NH 03824 USA. [Saleska, Scott] Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ USA. [de Oliveira, Raimundo Cosme, Jr.] EMBRAPA Amazonia Oriental, Belem, Para, Brazil. [Schietti, Juliana] Inst Nacl de Pesquisas da Amazonia, Manaus, Amazonas, Brazil. RP Hunter, MO (reprint author), Univ New Hampshire, Earth Sci Res Ctr, Durham, NH 03824 USA. EM Hunter.maria@gmail.com RI Keller, Michael/A-8976-2012; Morton, Douglas/D-5044-2012; Ducey, Mark/K-1101-2016 OI Keller, Michael/0000-0002-0253-3359; FU NASA [NNX09AO46H, NNG06GE11A, NNX09AI33G, NNG04G073G, NNX06AH36G]; NSF [DEB0721140]; USAid Sustainable Landscapes FX Funding provided by NASA Grant NNX09AO46H, NASA grant NNG06GE11A, NASA grant NNX09AI33G, NASA grant NNG04G073G, NASA grant NNX06AH36G, NSF grant DEB0721140, USAid Sustainable Landscapes (www.nasa.gov,www.nsf.gov,www.usaid.gov/climate/sustainablelandscapes). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 5 Z9 5 U1 4 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 13 PY 2015 VL 10 IS 7 AR e0132144 DI 10.1371/journal.pone.0132144 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1PW UT WOS:000358193100024 PM 26168242 ER PT J AU Overholt, WA Diaz, R Rosskopf, E Green, SJ Overholt, WA AF Overholt, Will A. Diaz, Rodrigo Rosskopf, Erin Green, Stefan J. Overholt, William A. TI Deep Characterization of the Microbiomes of Calophya spp. (Hemiptera: Calophyidae) Gall-Inducing Psyllids Reveals the Absence of Plant Pathogenic Bacteria and Three Dominant Endosymbionts SO PLOS ONE LA English DT Article ID 16S RIBOSOMAL-RNA; SAP-FEEDING INSECTS; PHLOEM-SAP; PROKARYOTIC ENDOSYMBIONTS; BRAZILIAN PEPPERTREE; DIAPHORINA-CITRI; LICE HEMIPTERA; GENOME; PSYLLOIDEA; SYMBIONTS AB Bacteria associated with sap-feeding insect herbivores include not only symbionts that may increase their hosts' fitness but also harmful plant pathogens. Calophya spp. gall-inducing psyllids (Hemiptera: Calophyidae) are being investigated for their potential as biological control agents of the noxious weed, Brazilian peppertree (Schinus terebinthifolia), in Florida. Although there are no examples of plant pathogen transmission by members of the family Calophyidae, several insects in the superfamily Psylloidea are known to transmit pathogenic bacteria in the genera Candidatus Liberibacter and Candidatus Phytoplasma. To determine whether Calophya spp. harbor potentially harmful plant pathogenic bacteria, we sequenced small subunit (SSU) ribosomal RNA (rRNA) gene amplicons generated from individuals from four Calophya spp. populations. All microbial SSU gene sequences fell into the bacterial domain, with 98-99% belonging to the Proteobacteria. The Calophya microbiomes contained a relatively simple community, with 49-79 operational taxonomic units (OTUs; 97%) detected, and only 5-8 OTUs with greater than 1% abundance. Candidatus Carsonella showed the highest relative abundance, with OTUs from this candidate genus representing between 51 - 65% of all recovered sequences. The next most abundant clade observed was an unclassified Enterobacteriacae group closely related to bacteria from the genera Buchnera and Blochmannia that ranged from 20-31% in relative abundance. Wolbachia populations were the third most abundant group and represented 7-27% of the diversity in microbial OTUs. No SSU rRNA gene sequences from putative pathogenic bacteria from the genera Ca. Liberibacter or Ca. Phytoplasma were detected in the microbiomes of the four Calophya populations. The probability that infected psyllids were present in our colonies, but were not sampled, was extremley low (1.39 x 10(-10)). As far as we are aware, our study is the first to characterize the microbiome of a candidate biological control agent, and coupled with previous work demonstrating a high degree of host specificity and absence of plant viruses, suggests that releasing Calophya spp. in United States poses minimal risk to non-target plants. C1 [Overholt, Will A.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Diaz, Rodrigo] Louisiana State Univ, Dept Entomol, Baton Rouge, LA 70803 USA. [Rosskopf, Erin] USDA, US Hort Res Lab, Ft Pierce, FL USA. [Green, Stefan J.] Univ Illinois, Dept Biol Sci, Chicago, IL 60680 USA. [Overholt, William A.] Univ Florida, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. RP Overholt, WA (reprint author), Univ Florida, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. EM billover@ufl.edu OI Green, Stefan/0000-0003-2781-359X FU Florida Fish and Wildlife Conservation Commission; Florida Department of Agriculture and Consumer Services; National Science Foundation Graduate Research Fellowship [2013172310] FX This research was funded in part by grants from the Florida Fish and Wildlife Conservation Commission, the Florida Department of Agriculture and Consumer Services, and a National Science Foundation Graduate Research Fellowship, https://www.nsfgrfp.org/, (to WAO1) under Grant No. 2013172310. Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 77 TC 0 Z9 0 U1 4 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 10 PY 2015 VL 10 IS 7 AR e0132248 DI 10.1371/journal.pone.0132248 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1FD UT WOS:000358162300107 PM 26161659 ER PT J AU Nam, HY Coates, B Kim, KS Park, M Lee, JH AF Nam, Hwa Yeun Coates, Brad Kim, Kyung Seok Park, Marana Lee, Joon-Ho TI Characterization of 12 Novel Microsatellite Markers of Sogatella furcifera (Hemiptera: Delphacidae) Identified From Next-Generation Sequence Data SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Sogatella furcifera; microsatellite marker; genetic diversity ID LEPIDOPTERA CRAMBIDAE POPULATIONS; NILAPARVATA-LUGENS; RICE PLANTHOPPERS; GENETIC-STRUCTURE; DNA; GENOME; REPEATS; INSECT; LOCI; DIFFERENTIATION AB The white-backed planthopper, Sogatella furcifera (Horvath) (Hemiptera: Delphacidae), is a major pest of rice and has long-range migratory behavior in Asia. Microsatellite markers (simple sequence repeats) have been widely used to determine the origins and genetic diversity of insect pests. We identified novel microsatellite loci for S. furcifera samples collected from Laos, Vietnam, and three localities in Bangladesh from next-generation Roche 454 pyrosequencing data. Size polymorphism at 12 microsatellite loci was verified for 40 adult individuals collected from Shinan, South Korea. The average number of alleles per locus was 7.92. The mean values of observed (H-o) and expected heterozygosities (H-E) were 0.615 and 0.757, respectively. These new microsatellite markers will be a resource for future ecological genetic studies of S. furcifera samples across more broad geographic regions in Asia and may assist in estimations of genetic differentiation and gene flow among populations for implementation of more effective management strategies to control this serious rice pest. C1 [Nam, Hwa Yeun; Park, Marana; Lee, Joon-Ho] Seoul Natl Univ, Dept Agr Biotechnol, Entomol Program, Seoul 151921, South Korea. [Coates, Brad; Kim, Kyung Seok] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit,Genet Lab 113, Ames, IA 50011 USA. [Lee, Joon-Ho] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151921, South Korea. RP Lee, JH (reprint author), Seoul Natl Univ, Dept Agr Biotechnol, Entomol Program, Seoul 151921, South Korea. EM jh7lee@snu.ac.kr FU Rural Development Administration in Korea [PJ00922907]; Brain Korea Plus; United States Department of Agriculture, Agricultural Research Service (USDA-ARS; CRIS Project) [3625-22000-017-00]; Iowa Agriculture and Home Economics Experiment Station, Ames, IA [3543] FX This research was supported by grants from Rural Development Administration in Korea (PJ00922907) and was partially supported by the Brain Korea Plus. Computational, and bioinformatic support for sequence data analysis was provided by the United States Department of Agriculture, 565 Agricultural 566 Research Service (USDA-ARS; CRIS Project 3625-22000-017-00), and the Iowa Agriculture 567 and Home Economics Experiment Station, Ames, IA (Project 3543). NR 61 TC 1 Z9 1 U1 2 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUL 10 PY 2015 VL 15 AR 94 DI 10.1093/jisesa/iev069 PG 7 WC Entomology SC Entomology GA CM5JW UT WOS:000357725100001 ER PT J AU Kerr, JT Pindar, A Galpern, P Packer, L Potts, SG Roberts, SM Rasmont, P Schweiger, O Colla, SR Richardson, LL Wagner, DL Gall, LF Sikes, DS Pantoja, A AF Kerr, Jeremy T. Pindar, Alana Galpern, Paul Packer, Laurence Potts, Simon G. Roberts, Stuart M. Rasmont, Pierre Schweiger, Oliver Colla, Sheila R. Richardson, Leif L. Wagner, David L. Gall, Lawrence F. Sikes, Derek S. Pantoja, Alberto TI Climate change impacts on bumblebees converge across continents SO SCIENCE LA English DT Article ID DIVERSITY; PATTERNS; SHIFTS; BOMBUS; BEES; HYMENOPTERA; LATITUDE; APIDAE AB For many species, geographical ranges are expanding toward the poles in response to climate change, while remaining stable along range edges nearest the equator. Using long-term observations across Europe and North America over 110 years, we tested for climate change-related range shifts in bumblebee species across the full extents of their latitudinal and thermal limits and movements along elevation gradients. We found cross-continentally consistent trends in failures to track warming through time at species' northern range limits, range losses from southern range limits, and shifts to higher elevations among southern species. These effects are independent of changing land uses or pesticide applications and underscore the need to test for climate impacts at both leading and trailing latitudinal and thermal limits for species. C1 [Kerr, Jeremy T.; Pindar, Alana] Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada. [Galpern, Paul] Univ Calgary, Fac Environm Design, Calgary, AB T2N 1N4, Canada. [Packer, Laurence] York Univ, Dept Biol, Toronto, ON M3J 2R7, Canada. [Potts, Simon G.; Roberts, Stuart M.] Univ Reading, Sch Agr Policy & Dev, Reading, Berks, England. [Rasmont, Pierre] Univ Mons, Dept Zool, B-7000 Mons, Belgium. [Schweiger, Oliver] UFZ Helmholtz Ctr Environm Res, Dept Community Ecol, Halle, Germany. [Colla, Sheila R.] Wildlife Preservat Canada, Guelph, ON, Canada. [Richardson, Leif L.] Univ Vermont, Gund Inst, Burlington, VT USA. [Wagner, David L.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT USA. [Gall, Lawrence F.] Yale Univ, Div Entomol, Peabody Museum Nat Hist, New Haven, CT USA. [Sikes, Derek S.] Univ Alaska Fairbanks, Univ Alaska Museum, Fairbanks, AK USA. [Pantoja, Alberto] ARS, USDA, Subarctic Agr Res Unit, Fairbanks, AK USA. RP Kerr, JT (reprint author), Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada. EM jkerr@uottawa.ca RI Schweiger, Oliver/B-4909-2008; OI Schweiger, Oliver/0000-0001-8779-2335; packer, laurence/0000-0002-1711-8203; Pindar, Alana/0000-0002-4273-3267 FU Natural Sciences and Engineering Research Council of Canada strategic network (CANPOLIN: Canadian Pollination Initiative); University of Ottawa Research Chair in Macroecology and Conservation FX This research was funded by the Natural Sciences and Engineering Research Council of Canada strategic network (CANPOLIN: Canadian Pollination Initiative) and Discovery Grant support and University of Ottawa Research Chair in Macroecology and Conservation to J.T.K. We are grateful to anonymous reviewers whose comments improved this paper and to P. Williams for advice and perspectives during development of the research. All data and supporting scripts are available from Dryad Digital Repository: doi: 10.5061/dryad.gf774. NR 26 TC 56 Z9 56 U1 55 U2 224 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JUL 10 PY 2015 VL 349 IS 6244 BP 177 EP 180 DI 10.1126/science.aaa7031 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CM4OM UT WOS:000357664300041 PM 26160945 ER PT J AU Schroyen, M Steibel, JP Koltes, JE Choi, I Raney, NE Eisley, C Fritz-Waters, E Reecy, JM Dekkers, JCM Rowland, RRR Lunney, JK Ernst, CW Tuggle, CK AF Schroyen, Martine Steibel, Juan P. Koltes, James E. Choi, Igseo Raney, Nancy E. Eisley, Christopher Fritz-Waters, Eric Reecy, James M. Dekkers, Jack C. M. Rowland, Robert R. R. Lunney, Joan K. Ernst, Catherine W. Tuggle, Christopher K. TI Whole blood microarray analysis of pigs showing extreme phenotypes after a porcine reproductive and respiratory syndrome virus infection SO BMC GENOMICS LA English DT Article DE Pig; PRRS; Microarray; Transcriptomics; WGCNA; PCIT; Immune response ID ALVEOLAR MACROPHAGES; HOST RESPONSE; ECONOMIC-IMPACT; BINDING-PROTEIN; GENE-EXPRESSION; UNITED-STATES; SYNDROME PRRS; LYMPH-NODE; NETWORKS; CELLS AB Background: The presence of variability in the response of pigs to Porcine Reproductive and Respiratory Syndrome virus (PRRSv) infection, and recent demonstration of significant genetic control of such responses, leads us to believe that selection towards more disease resistant pigs could be a valid strategy to reduce its economic impact on the swine industry. To find underlying molecular differences in PRRS susceptible versus more resistant pigs, 100 animals with extremely different growth rates and viremia levels after PRRSv infection were selected from a total of 600 infected pigs. A microarray experiment was conducted on whole blood RNA samples taken at 0, 4 and 7 days post infection (dpi) from these pigs. From these data, we examined associations of gene expression with weight gain and viral load phenotypes. The single nucleotide polymorphism (SNP) marker WUR10000125 (WUR) on the porcine 60 K SNP chip was shown to be associated with viral load and weight gain after PRRSv infection, and so the effect of the WUR10000125 (WUR) genotype on expression in whole blood was also examined. Results: Limited information was obtained through linear modeling of blood gene differential expression (DE) that contrasted pigs with extreme phenotypes, for growth or viral load or between animals with different WUR genotype. However, using network-based approaches, molecular pathway differences between extreme phenotypic classes could be identified. Several gene clusters of interest were found when Weighted Gene Co-expression Network Analysis (WGCNA) was applied to 4dpi contrasted with 0dpi data. The expression pattern of one such cluster of genes correlated with weight gain and WUR genotype, contained numerous immune response genes such as cytokines, chemokines, interferon type I stimulated genes, apoptotic genes and genes regulating complement activation. In addition, Partial Correlation and Information Theory (PCIT) identified differentially hubbed (DH) genes between the phenotypically divergent groups. GO enrichment revealed that the target genes of these DH genes are enriched in adaptive immune pathways. Conclusion: There are molecular differences in blood RNA patterns between pigs with extreme phenotypes or with a different WUR genotype in early responses to PRRSv infection, though they can be quite subtle and more difficult to discover with conventional DE expression analyses. Co-expression analyses such as WGCNA and PCIT can be used to reveal network differences between such extreme response groups. C1 [Schroyen, Martine; Koltes, James E.; Fritz-Waters, Eric; Reecy, James M.; Dekkers, Jack C. M.; Ernst, Catherine W.; Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Steibel, Juan P.; Raney, Nancy E.; Ernst, Catherine W.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Steibel, Juan P.] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI 48824 USA. [Choi, Igseo; Lunney, Joan K.] USDA ARS, Beltsville Agr Res Ctr, APDL, Beltsville, MD 20705 USA. [Eisley, Christopher] Iowa State Univ, Dept Stat, Ames, IA USA. [Rowland, Robert R. R.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. RP Steibel, JP (reprint author), Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. EM steibelj@msu.edu; cktuggle@iastate.edu FU USDA ARS funding; USDA NIFA [2010-65205-20433]; U.S. National Pork Board [07-233, 09-244, 10-033] FX This work was supported by USDA ARS funding and USDA NIFA grant # 2010-65205-20433. The PRRS Host Genetics Consortium (PHGC) samples were supported through grants #07-233, #09-244 and #10-033 from the U.S. National Pork Board. The authors would like to thank Dr. Zhiliang Hu for the annotation of the Pigoligoarray. NR 62 TC 3 Z9 3 U1 2 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 10 PY 2015 VL 16 AR 516 DI 10.1186/s12864-015-1741-8 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CM4EJ UT WOS:000357636800001 PM 26159815 ER PT J AU Bertran, K Thomas, C Guo, X Bublot, M Pritchard, N Regan, JT Cox, KM Gasdaska, JR Dickey, LF Kapczynski, DR Swayne, DE AF Bertran, Kateri Thomas, Colleen Guo, Xuan Bublot, Michel Pritchard, Nikki Regan, Jeffrey T. Cox, Kevin M. Gasdaska, John R. Dickey, Lynn F. Kapczynski, Darrell R. Swayne, David E. TI Expression of H5 hemagglutinin vaccine antigen in common duckweed (Lemna minor) protects against H5N1 high pathogenicity avian influenza virus challenge in immunized chickens SO VACCINE LA English DT Article DE Chickens; H5N1 high pathogenicity avian influenza; Hemagglutinin; Plant-derived vaccine ID REVERSE GENETICS; RECOMBINANT FOWLPOX; RECEPTOR-BINDING; EFFICACY; IMMUNOGENICITY; INFECTIONS; STRATEGIES; VARIANTS; ANTIBODY; POULTRY AB A synthetic hemagglutinin (HA) gene from the highly pathogenic avian influenza (HPAI) virus A/chicken/Indonesia/7/2003 (H5N1) (Indo/03) was expressed in aquatic plant Lemna minor (rLemna-HA). In Experiment 1, efficacy of rlemna-HA was tested on birds immunized with 0.2 mu g or 23 mu g HA and challenged with 10(6) mean chicken embryo infectious doses (EID50) of homologous virus strain. Both dosages of rLemna-HA conferred clinical protection and dramatically reduced viral shedding. Almost all the birds immunized with either dosage of rLemna-HA elicited HA antibody titers against Indo/03 antigen, suggesting an association between levels of anti-Indo/03 antibodies and protection. In Experiment 2, efficacy of rlemna-HA was tested on birds immunized with 0.9 mu g or 2.2 mu g HA and challenged with 10(6) EID50 of heterologous H5N1 virus strains A/chicken/Vietnam/NCVD-421/2010 (VN/10) or A/chicken/West Java/PM-WU/2006 (PWT/06). Birds challenged with VN/10 exhibited 100% survival regardless of immunization dosage, while birds challenged with PWT/06 had 50% and 30% mortality at 0.9 mu g HA and 2.2 mu g HA, respectively. For each challenge virus, viral shedding titers from 2.2 mu g HA vaccinated birds were significantly lower than those from 0.9 mu g HA vaccinated birds, and titers from both immunized groups were in turn significantly lower than those from sham vaccinated birds. Even if immunized birds elicited HA titers against the vaccine antigen Indo/03, only the groups challenged with VN/10 developed humoral immunity against the challenge antigen. None (rLemna-HA 0.9 mu g HA) and 40% (rLemna-HA 2.2 mu g HA) of the immunized birds challenged with PWT/06 elicited pre-challenge antibody titers, respectively. In conclusion, Lemna-expressed HA demonstrated complete protective immunity against homologous challenge and suboptimal protection against heterologous challenge, the latter being similar to results from inactivated whole virus vaccines. Transgenic duckweed-derived HA could be a good alternative for producing high quality antigen for an injectable vaccine against H5N1 HPAI viruses. Published by Elsevier Ltd. C1 [Bertran, Kateri; Thomas, Colleen; Kapczynski, Darrell R.; Swayne, David E.] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. [Guo, Xuan] Merial Ltd, R&D, Athens, GA 30601 USA. [Bublot, Michel] Merial SAS, R&D, F-69007 Lyon, France. [Pritchard, Nikki] Merial Select Inc, R&D, Gainesville, GA 30501 USA. [Regan, Jeffrey T.; Cox, Kevin M.; Gasdaska, John R.; Dickey, Lynn F.] Biolex Therapeut, Pittsboro, NC 27312 USA. RP Bertran, K (reprint author), ARS, Southeast Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM kateri.bertran@ars.usda.gov; colleen.thomas@fda.hhs.gov; Xuan.Guo@merial.com; Michel.bublot@merial.com; Nikki.Pritchard@merial.com; reganj75@gmail.com; kvcx@novozymes.com; jrgazman@gmail.com; lfdickey@gmail.com; darrell.kapczynski@ars.usda.gov; david.swayne@ars.usda.gov FU Merial, Inc. [58-6612-7-171]; Southeast Poultry Research Laboratory, Agricultural Research Service U.S. Department of Agriculture [58-6612-7-171] FX The authors would like to extend their gratitude to Karolyn Troupe at Merial Ltd. for vaccine preparation and characterization. Joan Beck, Kira Moresco, and James Doster at SEPRL for conducting the technical aspects of the animal study and analysis of clinical samples. This project was partially funded by Trust Agreement #58-6612-7-171 in collaboration between Merial, Inc. and Southeast Poultry Research Laboratory, Agricultural Research Service U.S. Department of Agriculture. This document is provided for scientific purposes only. Any reference to a brand of trademark herein is for informational purposes only and is not intended for a commercial purpose or to dilute the rights of the respective owner(s) of the brand(s) or trademark(s) (R) and (TM): all marks are the property of their respective owners. NR 45 TC 1 Z9 1 U1 3 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUL 9 PY 2015 VL 33 IS 30 BP 3456 EP 3462 DI 10.1016/j.vaccine.2015.05.076 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA CM7UO UT WOS:000357902200005 PM 26067184 ER PT J AU Stenfeldt, C Pacheco, JM Singanallur, NB Ferreira, HCD Vosloo, W Rodriguez, LL Arzt, J AF Stenfeldt, Carolina Pacheco, Juan M. Singanallur, Nagendrakumar B. Ferreira, Helena C. de Carvalho Vosloo, Wilna Rodriguez, Luis L. Arzt, Jonathan TI Clinical and virological dynamics of a serotype O 2010 South East Asia lineage foot-and-mouth disease virus in sheep using natural and simulated natural inoculation and exposure systems SO VETERINARY MICROBIOLOGY LA English DT Article DE FMDV; Foot-and-mouth disease; Virus; Sheep; Pathogenesis; Infection ID UNITED-KINGDOM; 2001 EPIDEMIC; CATTLE; INFECTION; PATHOGENESIS; IDENTIFICATION; DIAGNOSIS; ANTIBODY; SWINE; ASSAY AB Within-host infection dynamics of a recent field isolate of foot-and-mouth disease virus (FMDV), serotype O, topotype South East Asia, lineage Myamar'98 were evaluated in sheep using four different systems for virus exposure. Two novel, simulated natural, inoculation systems consisting of intra-nasopharyngeal (INP) deposition and aerosol inoculation were evaluated in comparison with two conventional systems: coronary band inoculation and direct contact exposure. All four exposure systems were efficient in generating consistently severe, generalized FMD with synchronous clinical characteristics within exposure groups, indicating that this Myanmar98 strain is highly virulent in sheep. Clinical and virological dynamics were similarly rapid following INP- and coronary band inoculation, with both systems leading to significantly earlier detection of virus shedding when compared to aerosol inoculation and contact exposure. The data presented herein support application of the two optimized simulated natural inoculation systems as valid alternatives to conventionally used exposure systems for studies of FMDV pathogenesis and vaccinology in sheep. Furthermore, the data suggest that targeted exposure of the ovine pharynx is highly efficient for generating consistent FMDV infection, which supports critical involvement of this anatomic region as a site of primary virus replication in sheep. Published by Elsevier B.V. C1 [Stenfeldt, Carolina; Pacheco, Juan M.; Ferreira, Helena C. de Carvalho; Rodriguez, Luis L.; Arzt, Jonathan] ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. [Stenfeldt, Carolina; Ferreira, Helena C. de Carvalho] PIADC Res Participat Program, Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Singanallur, Nagendrakumar B.; Vosloo, Wilna] CSIRO, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Arzt, J (reprint author), ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. EM Jonathan.Arzt@ars.usda.gov OI Stenfeldt, Carolina/0000-0002-2074-3886; Pacheco, Juan/0000-0001-5477-0201; Arzt, Jonathan/0000-0002-7517-7893 FU CSIRO-Australian Animals Health Laboratory [58-1940-3-004]; Agricultural Research Service, USDA [58-1940-3-004]; CSIRO; Australian Government [PSH 0652]; ARS-CRIS Project [1940- 32000-057-00D]; Plum Island Animal Disease Center Research Participation Program fellowships; Cattle Council of Australia; Australian Dairy Farmers; Australian Lot Feeders Association; Wool Producers Australia; Sheepmeat Council of Australia; Australian Pork Limited; Goat Industry Council of Australia through the Meat and Livestock Australia Donor Company FX This study was a collaboration between the CSIRO-Australian Animals Health Laboratory and the Agricultural Research Service, USDA, who co-funded the work under collaborative agreement 58-1940-3-004. Funding through CSIRO was provided in part by the livestock industries in Australia through the Cattle Council of Australia, Australian Dairy Farmers, Australian Lot Feeders Association, Wool Producers Australia, Sheepmeat Council of Australia, Australian Pork Limited and the Goat Industry Council of Australia through the Meat and Livestock Australia Donor Company matching arrangements with the Australian Government (Project PSH 0652). Animal Health Australia provided the project management service for all relevant industries. Additional funding came from ARS-CRIS Project 1940- 32000-057-00D. CS and HCF are recipients of Plum Island Animal Disease Center Research Participation Program fellowships, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the US Department of Energy. None of the funding sources had influence upon design or performance of experimental study, interpretation of results or writing of the manuscript. The Korean FMDV isolate was provided by Dr. Kwang-Nyeong Lee, Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs Republic of Korea. The authors thank Elizabeth Bishop, Ethan Hartwig and George Smoliga for processing of samples and Erin Howey for immunomicroscopy support. NR 35 TC 5 Z9 5 U1 4 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD JUL 9 PY 2015 VL 178 IS 1-2 BP 50 EP 60 DI 10.1016/j.vetmic.2015.04.004 PG 11 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CL2BU UT WOS:000356749400006 PM 25937316 ER PT J AU Kim, ES Sonstegard, TS Van Tassell, CP Wiggans, G Rothschild, MF AF Kim, Eui-Soo Sonstegard, Tad S. Van Tassell, Curtis P. Wiggans, George Rothschild, Max F. TI The Relationship between Runs of Homozygosity and Inbreeding in Jersey Cattle under Selection SO PLOS ONE LA English DT Article ID COMPLEX VERTEBRAL MALFORMATION; DAIRY-CATTLE; DEPRESSION; FERTILITY; GENOME; HOLSTEIN; MUTATION; TRAITS; OLD; HETEROZYGOSITY AB Inbreeding is often an inevitable outcome of strong directional artificial selection but on average it reduces population fitness with increased frequency of recessive deleterious alleles. Runs of homozygosity (ROH) representing genomic autozygosity that occur from mating between selected and genomically related individuals may be able to reveal the regions affecting fitness. To examine the influence of genomic autozygosity on fitness, we used a genome-wide association test to evaluate potential negative correlations between ROH and daughter pregnancy rate (DPR) or somatic cell score (SCS) in US Jersey cattle. In addition, relationships between changes of local ROH and inbreeding coefficients (F) were assessed to locate genomic regions with increased inbreeding. Despite finding some decreases in fertility associated with incremental increases in F, most emerging local ROH were not significantly associated with DPR or SCS. Furthermore, the analyses of ROH could be approximated with the most frequent haplotype(s), including the associations of ROH and F or traits. The analysis of the most frequent haplotype revealed that associations of ROH and fertility could be accounted for by the additive genetic effect on the trait. Thus, we suggest that a change of autozygosity is more likely to demonstrate footprints of selected haplotypes for production rather than highlight the possible increased local autozygosity of a recessive detrimental allele resulting from the mating between closely related animals in Jersey cattle. C1 [Kim, Eui-Soo; Sonstegard, Tad S.; Van Tassell, Curtis P.; Wiggans, George] ARS, Anim Genom & Improvement Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. [Kim, Eui-Soo; Rothschild, Max F.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. RP Rothschild, MF (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. EM mfrothsc@iastate.edu FU USDA-ARS-BFGL [1265-31000-104]; College of Agriculture and Life Sciences; State of Iowa and Hatch funds; Next-Generation BioGreen 21 Program [PJ011044]; Rural Development Administration, Republic of Korea FX This work was supported by the USDA-ARS-BFGL applied genomics project 1265-31000-104, the College of Agriculture and Life Sciences, the State of Iowa and Hatch funds. E.-S. Kim was partially supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ011044), Rural Development Administration, Republic of Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 3 Z9 3 U1 2 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 8 PY 2015 VL 10 IS 7 AR e0129967 DI 10.1371/journal.pone.0129967 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1EJ UT WOS:000358159700018 PM 26154171 ER PT J AU Kondo, MC Keene, D Hohl, BC MacDonald, JM Branas, CC AF Kondo, Michelle C. Keene, Danya Hohl, Bernadette C. MacDonald, John M. Branas, Charles C. TI A Difference-In-Differences Study of the Effects of a New Abandoned Building Remediation Strategy on Safety SO PLOS ONE LA English DT Article ID CRIME; HEALTH; DISORDER; VACANT; NEIGHBORHOODS; MULTILEVEL; IMPACTS; POLICY; LAND; CITY AB Vacant and abandoned buildings pose significant challenges to the health and safety of communities. In 2011 the City of Philadelphia began enforcing a Doors and Windows Ordinance that required property owners of abandoned buildings to install working doors and windows in all structural openings or face significant fines. We tested the effects of the new ordinance on the occurrence of crime surrounding abandoned buildings from January 2011 to April 2013 using a difference-in-differences approach. We used Poisson regression models to compare differences in pre- and post-treatment measures of crime for buildings that were remediated as a result of the ordinance (n = 676) or permitted for renovation (n = 241), and randomly-matched control buildings that were not remediated (n = 676) or permitted for renovation (n = 964), while also controlling for sociodemographic and other confounders measured around each building. Building remediations were significantly associated with citywide reductions in overall crimes, total assaults, gun assaults and nuisance crimes (p < 0.001). Building remediations were also significantly associated with reductions in violent gun crimes in one city section (p < 0.01). At the same time, some significant increases were seen in narcotics sales and possession and property crimes around remediated buildings (p < 0.001). Building renovation permits were significantly associated with reductions in all crime classifications across multiple city sections (p < 0.001). We found no significant spatial displacement effects. Doors and windows remediation offers a relatively low-cost method of reducing certain crimes in and around abandoned buildings. Cities with an abundance of decaying and abandoned housing stock might consider some form of this structural change to their built environments as one strategy to enhance public safety. C1 [Kondo, Michelle C.] US Forest Serv, USDA, No Res Stn, Philadelphia, MS 39350 USA. [Kondo, Michelle C.; Hohl, Bernadette C.; Branas, Charles C.] Univ Penn, Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [Keene, Danya] Yale Univ, Sch Publ Hlth, Social & Behav Sci, New Haven, CT USA. [MacDonald, John M.] Univ Penn, Sch Arts & Sci, Dept Criminol, Philadelphia, PA USA. RP Kondo, MC (reprint author), US Forest Serv, USDA, No Res Stn, Philadelphia, MS 39350 USA. EM michelleckondo@fs.fed.us OI Kondo, Michelle/0000-0001-9152-1589 FU Robert Wood Johnson Health and Societies Program; National Institutes of Health [R01AA020331]; Centers for Disease Control [R49CE002474] FX This study was funded in part by the Robert Wood Johnson Health and Societies Program, the National Institutes of Health (grant no. R01AA020331), and the Centers for Disease Control (grant no. R49CE002474). NR 32 TC 4 Z9 4 U1 5 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 8 PY 2015 VL 10 IS 7 AR e0129582 DI 10.1371/journal.pone.0129582 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1EJ UT WOS:000358159700014 PM 26153687 ER PT J AU Blaustein, RA Pachepsky, YA Hill, RL Shelton, DR AF Blaustein, Ryan A. Pachepsky, Yakov A. Hill, Robert L. Shelton, Daniel R. TI Solid Manure As a Source of Fecal Indicator Microorganisms: Release under Simulated Rainfall SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID ESCHERICHIA-COLI; MANAGEMENT-SYSTEMS; POULTRY LITTER; CATTLE MANURE; E. COLI; SOIL; RUNOFF; TRANSPORT; CRYPTOSPORIDIUM; ENTEROCOCCI AB Understanding and quantifying microbial release from manure is a precondition to estimation and management Of Microbial water quality. The objectives of this work were to determine the effects of rainfall intensity and surface slope on the release of Escherichia coil, enterococci, total coliforms, and dissolved chloride from solid dairy manure and to assess the performance of the one-parametric exponential model and the two-parametric Bradford-Schijven model when simulating the observed release. A controlled-intensity rainfall simulator induced 1 h of release in runoff/leachate partitioning boxes at three rainfall intensities (30, 60, and 90 mm h(-1)) and two surface slopes (5% and 20%). Bacterial concentrations in initial release were more than 1 order of magnitude lower than their starting concentrations in manure. As bacteria were released, they were partitioned into runoff and leachate at similar concentrations, but in different volumes, depending on slope. Bacterial release occurred in two stages that corresponded to mechanisms associated with release of manure liquid- and solid-phases. Parameters of the two models fitted to the bacterial release dependencies on rainfall depth were not significantly affected by rainfall intensity or slope. Based on model performance tests, the Bradford-Schijven model is recommended for simulating bacterial release from solid manure. C1 [Blaustein, Ryan A.; Hill, Robert L.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Blaustein, Ryan A.; Pachepsky, Yakov A.; Shelton, Daniel R.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Blaustein, RA (reprint author), Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. OI Pachepsky, Yakov/0000-0003-0232-6090 NR 37 TC 4 Z9 4 U1 0 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 7 PY 2015 VL 49 IS 13 BP 7860 EP 7869 DI 10.1021/acs.est.5b01095 PG 10 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CM6ZN UT WOS:000357840300045 PM 26011817 ER PT J AU Hanes, RJ Craze, NB Goel, PK Bakshi, BR AF Hanes, Rebecca J. Craze, Nathan B. Goel, Prem K. Bakshi, Bhavik R. TI Allocation Games: Addressing the III-Posed Nature of Allocation in Life-Cycle Inventories SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SYSTEM EXPANSION; ETHANOL; CORN; CONVERSION; INTENSITY; PRODUCTS AB Allocation is required when a life cycle contains multi-functional processes. One approach to allocation is to partition the embodied resources in proportion to a criterion, such as product mass or cost. Many practitioners apply multiple partitioning criteria to avoid choosing one arbitrarily. However, life cycle results from different allocation methods frequently contradict each other, making it difficult or impossible for the practitioner to draw any meaningful conclusions from the study. Using the matrix notation for life-cycle inventory data, we show that an inventory that requires allocation leads to an ill-posed problem: an inventory based on allocation is one of an infinite number of inventories that are highly dependent upon allocation methods. This insight is applied to comparative life-cycle assessment (LCA), in which products with the same function but different life cycles are compared. Recently, there have been several studies that applied multiple allocation methods and found that different products were preferred under different methods. We develop. the Comprehensive Allocation Investigation Strategy (CAIS) to examine any given inventory under all possible allocation decisions, enabling us to detect comparisons that are not robust to allocation, even when the comparison appears robust under conventional partitioning methods. While CATS does not solve the ill-posed problem, it provides a systematic way to parametrize and examine the effects of partitioning allocation. The practical usefulness of this approach is demonstrated with two case studies. The first compares ethanol produced from corn stover hydrolysis, corn stover gasification, and corn grain fermentation. This comparison was not robust to allocation. The second case study compares 1,3-propanediol (PDO) produced from fossil fuels and from biomass, which was found to be a robust comparison. C1 [Hanes, Rebecca J.; Bakshi, Bhavik R.] Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 USA. [Goel, Prem K.] Ohio State Univ, Dept Stat, Columbus, OH 43210 USA. [Craze, Nathan B.] USDA, NASS, Washington, DC 20004 USA. RP Bakshi, BR (reprint author), Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 USA. EM bakshi.2@osu.edu FU National Science Foundation [CBET-0829026]; United States Department of Agriculture [2012-38202-19288] FX Partial funding for this work was provided by the National Science Foundation (CBET-0829026) and the United States Department of Agriculture (2012-38202-19288). NR 39 TC 3 Z9 3 U1 1 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 7 PY 2015 VL 49 IS 13 BP 7996 EP 8003 DI 10.1021/acs.est.5b01192 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CM6ZN UT WOS:000357840300061 PM 26061700 ER PT J AU O'Rourke, JA Fu, FL Bucciarelli, B Yang, SS Samac, DA Lamb, JFS Monteros, MJ Graham, MA Gronwald, JW Krom, N Li, J Dai, XB Zhao, PX Vance, CP AF O'Rourke, Jamie A. Fu, Fengli Bucciarelli, Bruna Yang, S. Sam Samac, Deborah A. Lamb, Joann F. S. Monteros, Maria J. Graham, Michelle A. Gronwald, John W. Krom, Nick Li, Jun Dai, Xinbin Zhao, Patrick X. Vance, Carroll P. TI The Medicago sativa gene index 1.2: a web-accessible gene expression atlas for investigating expression differences between Medicago sativa subspecies SO BMC GENOMICS LA English DT Article DE Medicago sativa; Illumina; RNA-seq; Gene expression atlas; Cysteine cluster protein; Nodule-specific cysteine-rich peptide ID DOMAIN TRANSCRIPTION FACTOR; RNA-SEQ; ARABIDOPSIS-THALIANA; TETRAPLOID ALFALFA; COLD-ACCLIMATION; PROVIDES INSIGHT; VESSEL FORMATION; GENOME SEQUENCE; LOW-TEMPERATURE; WINTER-WHEAT AB Background: Alfalfa (Medicago sativa L.) is the primary forage legume crop species in the United States and plays essential economic and ecological roles in agricultural systems across the country. Modern alfalfa is the result of hybridization between tetraploid M. sativa ssp. sativa and M. sativa ssp. falcata. Due to its large and complex genome, there are few genomic resources available for alfalfa improvement. Results: A de novo transcriptome assembly from two alfalfa subspecies, M. sativa ssp. sativa (B47) and M. sativa ssp. falcata (F56) was developed using Illumina RNA-seq technology. Transcripts from roots, nitrogen-fixing root nodules, leaves, flowers, elongating stem internodes, and post-elongation stem internodes were assembled into the Medicago sativa Gene Index 1.2 (MSGI 1.2) representing 112,626 unique transcript sequences. Nodule-specific and transcripts involved in cell wall biosynthesis were identified. Statistical analyses identified 20,447 transcripts differentially expressed between the two subspecies. Pair-wise comparisons of each tissue combination identified 58,932 sequences differentially expressed in B47 and 69,143 sequences differentially expressed in F56. Comparing transcript abundance in floral tissues of B47 and F56 identified expression differences in sequences involved in anthocyanin and carotenoid synthesis, which determine flower pigmentation. Single nucleotide polymorphisms (SNPs) unique to each M. sativa subspecies (110,241) were identified. Conclusions: The Medicago sativa Gene Index 1.2 increases the expressed sequence data available for alfalfa by ninefold and can be expanded as additional experiments are performed. The MSGI 1.2 transcriptome sequences, annotations, expression profiles, and SNPs were assembled into the Alfalfa Gene Index and Expression Database (AGED) at http://plantgrn.noble.org/AGED/, a publicly available genomic resource for alfalfa improvement and legume research. C1 [O'Rourke, Jamie A.; Graham, Michelle A.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Fu, Fengli; Vance, Carroll P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Bucciarelli, Bruna; Yang, S. Sam; Samac, Deborah A.; Lamb, Joann F. S.; Gronwald, John W.; Vance, Carroll P.] USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. [Monteros, Maria J.; Krom, Nick; Li, Jun; Dai, Xinbin; Zhao, Patrick X.] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. RP O'Rourke, JA (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM Jamie.ORourke@ars.usda.gov FU USDA-ARS [3640-12210-002-00D, 3640-21000-029-00D, 3625-21220-005-00D] FX This work was performed in part using resources at the University of Minnesota Supercomputing Institute. This project was supported by USDA-ARS projects 3640-12210-002-00D, 3640-21000-029-00D and 3625-21220-005-00D. The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 105 TC 6 Z9 6 U1 2 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 7 PY 2015 VL 16 AR 502 DI 10.1186/s12864-015-1718-7 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CM3DF UT WOS:000357561200001 PM 26149169 ER PT J AU Rodrigues, FA Fuganti-Pagliarini, R Marcolino-Gomes, J Nakayama, TJ Molinari, HBC Lobo, FP Harmon, FG Nepomuceno, AL AF Rodrigues, Fabiana Aparecida Fuganti-Pagliarini, Renata Marcolino-Gomes, Juliana Nakayama, Thiago Jonas Correa Molinari, Hugo Bruno Lobo, Francisco Pereira Harmon, Frank G. Nepomuceno, Alexandre Lima TI Daytime soybean transcriptome fluctuations during water deficit stress SO BMC GENOMICS LA English DT Article DE Abiotic stress; Daily oscillation; diel regulation; Drought; Glycine max; Plant metabolism ID TIME QUANTITATIVE PCR; GENE-EXPRESSION AB Background: Since drought can seriously affect plant growth and development and little is known about how the oscillations of gene expression during the drought stress-acclimation response in soybean is affected, we applied Illumina technology to sequence 36 cDNA libraries synthesized from control and drought-stressed soybean plants to verify the dynamic changes in gene expression during a 24-h time course. Cycling variables were measured from the expression data to determine the putative circadian rhythm regulation of gene expression. Results: We identified 4866 genes differentially expressed in soybean plants in response to water deficit. Of these genes, 3715 were differentially expressed during the light period, from which approximately 9.55 % were observed in both light and darkness. We found 887 genes that were either up-or down-regulated in different periods of the day. Of 54,175 predicted soybean genes, 35.52 % exhibited expression oscillations in a 24 h period. This number increased to 39.23 % when plants were submitted to water deficit. Major differences in gene expression were observed in the control plants from late day (ZT16) until predawn (ZT20) periods, indicating that gene expression oscillates during the course of 24 h in normal development. Under water deficit, dissimilarity increased in all time-periods, indicating that the applied stress influenced gene expression. Such differences in plants under stress were primarily observed in ZT0 (early morning) to ZT8 (late day) and also from ZT4 to ZT12. Stress-related pathways were triggered in response to water deficit primarily during midday, when more genes were up-regulated compared to early morning. Additionally, genes known to be involved in secondary metabolism and hormone signaling were also expressed in the dark period. Conclusions: Gene expression networks can be dynamically shaped to acclimate plant metabolism under environmental stressful conditions. We have identified putative cycling genes that are expressed in soybean leaves under normal developmental conditions and genes whose expression oscillates under conditions of water deficit. These results suggest that time of day, as well as light and temperature oscillations that occur considerably affect the regulation of water deficit stress response in soybean plants. C1 [Rodrigues, Fabiana Aparecida; Fuganti-Pagliarini, Renata; Marcolino-Gomes, Juliana; Nakayama, Thiago Jonas; Nepomuceno, Alexandre Lima] Brazilian Agr Res Corp Embrapa Soybean, BR-86001970 Londrina, PR, Brazil. [Marcolino-Gomes, Juliana] Univ Estadual Londrina, Dept Biol, Londrina, PR, Brazil. [Nakayama, Thiago Jonas] Univ Fed Vicosa, Dept Crop Sci, Vicosa, MG, Brazil. [Correa Molinari, Hugo Bruno] Embrapa Agroenergy CNPAE, Genet & Biotechnol Lab, Brasilia, DF, Brazil. [Lobo, Francisco Pereira] Brazilian Agr Res Corp Embrapa Agr Informat, Campinas, SP, Brazil. [Harmon, Frank G.] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA USA. [Correa Molinari, Hugo Bruno; Nepomuceno, Alexandre Lima] USDA ARS, Ctr Plant Gene Express, Embrapa LABEX US Plant Biotechnol, Albany, CA 94710 USA. RP Nepomuceno, AL (reprint author), Brazilian Agr Res Corp Embrapa Soybean, Embrapa Soybean Rod,Carlos Joao Str S-N, BR-86001970 Londrina, PR, Brazil. EM alexandre.nepomuceno@embrapa.br OI Fuganti-Pagliarini, Renata /0000-0001-9282-2826 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq [PDE-202211/2011-8, PDE-202639/2011-8]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES [8075/11-5] FX Embrapa-Labex USA funded this project Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq (project # PDE-202211/2011-8, PDE-202639/2011-8, respectively) granted scholarships for FAR and RFP. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES (process # 8075/11-5) granted scholarship for JMG. We thank the Plant Gene Expression Center - USDA-ARS, Albany-CA, for the laboratory facilities. Approved for publication by the Editorial Board of Embrapa Soja as manuscript 08/2015. NR 12 TC 2 Z9 2 U1 1 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUL 7 PY 2015 VL 16 AR 505 DI 10.1186/s12864-015-1731-x PG 19 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CM1OJ UT WOS:000357449900001 PM 26149272 ER PT J AU Fay, PA Prober, SM Harpole, WS Knops, JMH Bakker, JD Borer, ET Lind, EM MacDougall, AS Seabloom, EW Wragg, PD Adler, PB Blumenthal, DM Buckley, Y Chu, CJ Cleland, EE Collins, SL Davies, KF Du, GZ Feng, XH Firn, J Gruner, DS Hagenah, N Hautier, Y Heckman, RW Jin, VL Kirkman, KP Klein, J Ladwig, LM Li, Q McCulley, RL Melbourne, BA Mitchell, CE Moore, JL Morgan, JW Risch, AC Schutz, M Stevens, CJ Wedin, DA Yang, LH AF Fay, Philip A. Prober, Suzanne M. Harpole, W. Stanley Knops, Johannes M. H. Bakker, Jonathan D. Borer, Elizabeth T. Lind, Eric M. MacDougall, Andrew S. Seabloom, Eric W. Wragg, Peter D. Adler, Peter B. Blumenthal, Dana M. Buckley, YvonneM. Chu, Chengjin Cleland, Elsa E. Collins, Scott L. Davies, Kendi F. Du, Guozhen Feng, Xiaohui Firn, Jennifer Gruner, Daniel S. Hagenah, Nicole Hautier, Yann Heckman, Robert W. Jin, Virginia L. Kirkman, Kevin P. Klein, Julia Ladwig, Laura M. Li, Qi McCulley, Rebecca L. Melbourne, Brett A. Mitchell, Charles E. Moore, Joslin L. Morgan, John W. Risch, Anita C. Schuetz, Martin Stevens, Carly J. Wedin, David A. Yang, Louie H. TI Grassland productivity limited by multiple nutrients SO NATURE PLANTS LA English DT Article ID NITROGEN LIMITATION; TERRESTRIAL ECOSYSTEMS; CO-LIMITATION; PHOSPHORUS; RESPONSES; DEPOSITION; PLANT; BIODIVERSITY; IMPACTS; ECOLOGY AB Terrestrial ecosystem productivity is widely accepted to be nutrient limited(1). Although nitrogen (N) is deemed a key determinant of aboveground net primary production (ANPP) 2,3, the prevalence of co-limitation by N and phosphorus (P) is increasingly recognized(4-8). However, the extent to which terrestrial productivity is co-limited by nutrients other than N and P has remained unclear. Here, we report results from a standardized factorial nutrient addition experiment, in which we added N, P and potassium (K) combined with a selection of micronutrients (K+mu), alone or in concert, to 42 grassland sites spanning five continents, and monitored ANPP. Nutrient availability limited productivity at 31 of the 42 grassland sites. And pairwise combinations of N, P, and K+mu co-limited ANPP at 29 of the sites. Nitrogen limitation peaked in cool, high latitude sites. Our findings highlight the importance of less studied nutrients, such as K and micronutrients, for grassland productivity, and point to significant variations in the type and degree of nutrient limitation. We suggest that multiple-nutrient constraints must be considered when assessing the ecosystem-scale consequences of nutrient enrichment. C1 [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Prober, Suzanne M.] CSIRO Land & Water Flagship, Wembley, WA 6913, Australia. [Harpole, W. Stanley] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Harpole, W. Stanley] German Ctr Integrat Biodivers Res iDiv, D-04103 Leipzig, Germany. [Harpole, W. Stanley] UFZ Helmholtz Ctr Environm Res, Dept Physiol Divers, D-04318 Leipzig, Germany. [Harpole, W. Stanley] Univ Halle Wittenberg, Inst Biol, D-06108 Halle, Saale, Germany. [Knops, Johannes M. H.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [Bakker, Jonathan D.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Borer, Elizabeth T.; Lind, Eric M.; Seabloom, Eric W.; Wragg, Peter D.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [MacDougall, Andrew S.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Adler, Peter B.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Adler, Peter B.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Buckley, YvonneM.] Univ Dublin Trinity Coll, Trinity Ctr Biodivers Res, Sch Nat Sci, Zool, Dublin 2, Ireland. [Chu, Chengjin; Du, Guozhen] Lanzhou Univ, Res Stn Alpine Meadow & Wetland Ecosyst, Lanzhou 730000, Peoples R China. [Cleland, Elsa E.] Univ Calif San Diego, Ecol Behav & Evolut Sect, San Diego, CA 92093 USA. [Collins, Scott L.; Ladwig, Laura M.] Univ New Mexico, Dept Biol, Albuquerque, NM 88003 USA. [Davies, Kendi F.; Melbourne, Brett A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Feng, Xiaohui] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Firn, Jennifer] Queensland Univ Technol, Sch Earth Environm & Biol Sci, Brisbane, Qld 4001, Australia. [Gruner, Daniel S.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Hagenah, Nicole; Kirkman, Kevin P.] Univ KwaZulu Natal, Sch Life Sci, ZA-3209 Pietermaritzburg, South Africa. [Hautier, Yann] Univ Utrecht, Dept Biol, Ecol & Biodivers Grp, NL-3584 CH Utrecht, Netherlands. [Heckman, Robert W.; Mitchell, Charles E.] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA. [Jin, Virginia L.] USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68538 USA. [Klein, Julia] Colorado State Univ, Dept Forest Rangeland & Watershed Stewardship, Ft Collins, CO 80523 USA. [Li, Qi] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China. [McCulley, Rebecca L.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Moore, Joslin L.] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia. [Morgan, John W.] La Trobe Univ, Dept Bot, Bundoora, Vic 3083, Australia. [Risch, Anita C.; Schuetz, Martin] Swiss Fed Inst Forest Snow & Landscape Res, Community Ecol, CH-8903 Birmensdorf, Switzerland. [Stevens, Carly J.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Wedin, David A.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Yang, Louie H.] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA. RP Fay, PA (reprint author), USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. EM philip.fay@ars.usda.gov RI Gruner, Daniel/A-5166-2010; Buckley, Yvonne/B-1281-2008; iDiv, Deutsches Zentrum/B-5164-2016; Harpole, William/C-2814-2013; Mitchell, Charles/I-3709-2014; MacDougall, Andrew/F-2037-2011; Risch, Anita/A-9836-2012; Collins, Scott/P-7742-2014; Prober, Suzanne/G-6465-2010; OI Gruner, Daniel/0000-0002-3153-4297; Buckley, Yvonne/0000-0001-7599-3201; Harpole, William/0000-0002-3404-9174; Mitchell, Charles/0000-0002-1633-1993; Risch, Anita/0000-0003-0531-8336; Collins, Scott/0000-0002-0193-2892; Fay, Philip/0000-0002-8291-6316; Lind, Eric/0000-0003-3051-7724; Borer, Elizabeth/0000-0003-2259-5853; Heckman, Robert/0000-0002-2281-3091 FU National Science Foundation Research Coordination Network [NSF-DEB-1042132]; Long Term Ecological Research program [NSF-DEB-1234162]; Institute on the Environment [DG-0001-13] FX We thank the Minnesota Supercomputer Institute for hosting project data, the University of Minnesota Institute on the Environment for hosting Nutrient Network meetings, and each site investigator for funding their site-level operations. Network coordination and data management were supported by funds from the National Science Foundation Research Coordination Network (NSF-DEB-1042132) to E.T.B. and E.W.S., from the Long Term Ecological Research program (NSF-DEB-1234162) to the Cedar Creek LTER, and from the Institute on the Environment (DG-0001-13). P.A.F. acknowledges USDA-NIFA (2010-65615-20632). USDA is an equal opportunity employer and provider. NR 30 TC 26 Z9 27 U1 29 U2 109 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD JUL 6 PY 2015 VL 1 IS 7 AR UNSP 15080 DI 10.1038/NPLANTS.2015.80 PG 5 WC Plant Sciences SC Plant Sciences GA CV6UL UT WOS:000364406600001 PM 27250253 ER PT J AU Howard, JK Klausmeyer, KR Fesenmyer, KA Furnish, J Gardali, T Grantham, T Katz, JVE Kupferberg, S McIntyre, P Moyle, PB Ode, PR Peek, R Quinones, RM Rehn, AC Santos, N Schoenig, S Serpa, L Shedd, JD Slusark, J Viers, JH Wright, A Morrison, SA AF Howard, Jeanette K. Klausmeyer, Kirk R. Fesenmyer, Kurt A. Furnish, Joseph Gardali, Thomas Grantham, Ted Katz, Jacob V. E. Kupferberg, Sarah McIntyre, Patrick Moyle, Peter B. Ode, Peter R. Peek, Ryan Quinones, Rebecca M. Rehn, Andrew C. Santos, Nick Schoenig, Steve Serpa, Larry Shedd, Jackson D. Slusark, Joe Viers, Joshua H. Wright, Amber Morrison, Scott A. TI Patterns of Freshwater Species Richness, Endemism, and Vulnerability in California SO PLOS ONE LA English DT Article ID GLOBAL DECLINE; UNITED-STATES; CONSERVATION; BIODIVERSITY; EXTINCTIONS; CHALLENGES; THREATS AB The ranges and abundances of species that depend on freshwater habitats are declining worldwide. Efforts to counteract those trends are often hampered by a lack of information about species distribution and conservation status and are often strongly biased toward a few well-studied groups. We identified the 3,906 vascular plants, macroinvertebrates, and vertebrates native to California, USA, that depend on fresh water for at least one stage of their life history. We evaluated the conservation status for these taxa using existing government and non-governmental organization assessments (e.g., endangered species act, NatureServe), created a spatial database of locality observations or distribution information from similar to 400 data sources, and mapped patterns of richness, endemism, and vulnerability. Although nearly half of all taxa with conservation status (n = 1,939) are vulnerable to extinction, only 114 (6%) of those vulnerable taxa have a legal mandate for protection in the form of formal inclusion on a state or federal endangered species list. Endemic taxa are at greater risk than non-endemics, with 90% of the 927 endemic taxa vulnerable to extinction. Records with spatial data were available for a total of 2,276 species (61%). The patterns of species richness differ depending on the taxonomic group analyzed, but are similar across taxonomic level. No particular taxonomic group represents an umbrella for all species, but hotspots of high richness for listed species cover 40% of the hotspots for all other species and 58% of the hotspots for vulnerable freshwater species. By mapping freshwater species hotspots we show locations that represent the top priority for conservation action in the state. This study identifies opportunities to fill gaps in the evaluation of conservation status for freshwater taxa in California, to address the lack of occurrence information for nearly 40% of freshwater taxa and nearly 40% of watersheds in the state, and to implement adequate protections for freshwater taxa where they are currently lacking. C1 [Howard, Jeanette K.; Klausmeyer, Kirk R.; Serpa, Larry; Shedd, Jackson D.; Morrison, Scott A.] Nature Conservancy, San Francisco, CA 94123 USA. [Fesenmyer, Kurt A.] Trout Unltd, Boise, ID USA. [Furnish, Joseph] US Forest Serv, USDA, Vallejo, CA USA. [Gardali, Thomas] Point Blue Conservat Sci, Petaluma, CA USA. [Grantham, Ted; Katz, Jacob V. E.; Moyle, Peter B.; Peek, Ryan; Quinones, Rebecca M.; Santos, Nick] Univ Calif Davis, Ctr Watershed Sci, Davis, CA 95616 USA. [Grantham, Ted; Katz, Jacob V. E.; Moyle, Peter B.; Peek, Ryan; Quinones, Rebecca M.; Santos, Nick] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [Kupferberg, Sarah] Univ Calif Berkeley, Integrat Biol, Berkeley, CA 94720 USA. [McIntyre, Patrick; Rehn, Andrew C.; Schoenig, Steve; Slusark, Joe] Calif Dept Fish & Wildlife, Biogeog Data Branch, Sacramento, CA USA. [Ode, Peter R.] Calif Dept Fish & Wildlife, Aquat Bioassessment Lab, Rancho Cordova, CA USA. [Viers, Joshua H.] Univ Calif Merced, Sch Engn, Merced, CA USA. [Wright, Amber] Univ Hawaii Manoa, Dept Biol, Honolulu, HI 96822 USA. RP Howard, JK (reprint author), Nature Conservancy, San Francisco, CA 94123 USA. EM jeanette_howard@tnc.org OI Peek, Ryan/0000-0002-9577-6885 FU U. S. Bureau of Land Management FX We thank The Nature Conservancy for supporting development of this database and this research, with additional support from the U. S. Bureau of Land Management. With the exception of the authors, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 3 Z9 3 U1 2 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 6 PY 2015 VL 10 IS 7 AR e0130710 DI 10.1371/journal.pone.0130710 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1DQ UT WOS:000358157600044 PM 26147215 ER PT J AU Niu, JY Sahni, S Liao, SS Tucker, KL Dawson-Hughes, B Gao, X AF Niu, Jinya Sahni, Shivani Liao, Susu Tucker, Katherine L. Dawson-Hughes, Bess Gao, Xiang TI Association between Sleep Duration, Insomnia Symptoms and Bone Mineral Density in Older Boston Puerto Rican Adults SO PLOS ONE LA English DT Article ID C-REACTIVE PROTEIN; CARDIOVASCULAR RISK; PHYSICAL-ACTIVITY; OSTEOPOROSIS; INFLAMMATION; BIOMARKERS; FRACTURES; WOMEN; METAANALYSIS; QUALITY AB Objective To examine the association between sleep patterns (sleep duration and insomnia symptoms) and total and regional bone mineral density (BMD) among older Boston Puerto Rican adults. Materials/Methods We conducted a cross-sectional study including 750 Puerto Rican adults, aged 47-79 y living in Massachusetts. BMD at 3 hip sites and the lumbar spine were measured using dual-energy X-ray absorptiometry. Sleep duration (<= 5 h, 6 h, 7 h, 8 h, or <= 9 h/d) and insomnia symptoms (difficulty initiating sleep, difficulty maintaining sleep, early-morning awaking, and non-restorative sleep) were assessed by a questionnaire. Multivariable regression was used to examine sex-specific associations between sleep duration, insomnia symptoms and BMD adjusting for standard confounders and covariates. Results Men who slept >= 9h/d had significantly lower femoral neck BMD, relative to those reporting 8 h/d sleep, after adjusting for age, education level, smoking, physical activity, depressive symptomatology, comorbidity and serum vitamin D concentration. This association was attenuated and lost significance after further adjustment for urinary cortisol and serum inflammation biomarkers. In contrast, the association between sleep duration and BMD was not significant in women. Further, we did not find any significant associations between insomnia symptoms and BMD in men or women. Conclusions Our study does not support the hypothesis that shorter sleep duration and insomnia symptoms are associated with lower BMD levels in older adults. However, our results should be interpreted with caution. Future studies with larger sample size, objective assessment of sleep pattern, and prospective design are needed before a conclusion regarding sleep and BMD can be reached. C1 [Niu, Jinya; Liao, Susu] Chinese Acad Med Sci, Inst Basic Med Sci, Dept Epidemiol & Biostat, Peking Union Med Coll, Beijing 100730, Peoples R China. [Sahni, Shivani] Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Inst Aging Res,Hebrew SeniorLife, Boston, MA 02215 USA. [Tucker, Katherine L.] Univ Massachusetts Lowell, Dept Clin Lab & Nutr Sci, Boston, MA USA. [Dawson-Hughes, Bess] Tufts Univ, Bone Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gao, Xiang] Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. RP Gao, X (reprint author), Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. EM xxg14@psu.edu OI Sahni, Shivani/0000-0002-9827-0986 FU National Institutes of Health [P01 AG023394, P50 HL105185, R01 AG027087, R01 NS062879-01A2] FX The study was supported by grants (P01 AG023394, P50 HL105185, R01 AG027087, and R01 NS062879-01A2) from the National Institutes of Health. None of the sponsors participated in the design of study or in the collection, analysis, or interpretation of the data. NR 35 TC 1 Z9 1 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 6 PY 2015 VL 10 IS 7 AR e0132342 DI 10.1371/journal.pone.0132342 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1DQ UT WOS:000358157600247 PM 26147647 ER PT J AU Sainju, UM Allen, BL Caesar-TonThat, T Lenssen, AW AF Sainju, Upendra M. Allen, Brett L. Caesar-TonThat, Thecan Lenssen, Andrew W. TI Dryland soil chemical properties and crop yields affected by long-term tillage and cropping sequence SO SPRINGERPLUS LA English DT Article DE Chemical properties; Crop yields; Nutrients; Tillage; Crop rotation ID NORTHERN GREAT-PLAINS; NITROGEN-FERTILIZATION; WINTER-WHEAT; SPRING WHEAT; GRAIN; ROTATION; CARBON; ACIDIFICATION; SYSTEMS AB Information on the effect of long-term management on soil nutrients and chemical properties is scanty. We examined the 30-year effect of tillage frequency and cropping sequence combination on dryland soil Olsen-P, K, Ca, Mg, Na, SO4-S, and Zn concentrations, pH, electrical conductivity (EC), and cation exchange capacity (CEC) at the 0-120 cm depth and annualized crop yield in the northern Great Plains, USA. Treatments were no-till continuous spring wheat (Triticum aestivum L.) (NTCW), spring till continuous spring wheat (STCW), fall and spring till continuous spring wheat (FSTCW), fall and spring till spring wheat-barley (Hordeum vulgare L., 1984-1999) followed by spring wheat-pea (Pisum sativum L., 2000-2013) (FSTW-B/P), and spring till spring wheat-fallow (STW-F, traditional system). At 0-7.5 cm, P, K, Zn, Na, and CEC were 23-60% were greater, but pH, buffer pH, and Ca were 6-31% lower in NTCW, STCW, and FSTW-B/P than STW-F. At 7.5-15 cm, K was 23-52% greater, but pH, buffer pH, and Mg were 3-21% lower in NTCW, STCW, FSTCW, FSTW-B/P than STW-F. At 60-120 cm, soil chemical properties varied with treatments. Annualized crop yield was 23-30% lower in STW-F than the other treatments. Continuous N fertilization probably reduced soil pH, Ca, and Mg, but greater crop residue returned to the soil increased P, K, Na, Zn, and CEC in NTCW and STCW compared to STW-F. Reduced tillage with continuous cropping may be adopted for maintaining long-term soil fertility and crop yields compared with the traditional system. C1 [Sainju, Upendra M.; Allen, Brett L.; Caesar-TonThat, Thecan] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Lenssen, Andrew W.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Sainju, UM (reprint author), USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov NR 33 TC 5 Z9 5 U1 2 U2 15 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 2193-1801 J9 SPRINGERPLUS JI SpringerPlus PD JUL 5 PY 2015 VL 4 AR 320 DI 10.1186/s40064-015-1122-4 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CO7UT UT WOS:000359368700001 PM 26171303 ER PT J AU Hildebrandt, E Dunn, JR Cheng, HH AF Hildebrandt, Evin Dunn, John R. Cheng, Hans H. TI Addition of a UL5 helicase-primase subunit point mutation eliminates bursal-thymic atrophy of Marek's disease virus increment Meq recombinant virus but reduces vaccinal protection SO AVIAN PATHOLOGY LA English DT Article ID LYMPHOID ORGAN ATROPHY; CELL-CULTURE; IN-VITRO; REPLICATION; ATTENUATION; EFFICACY; PASSAGE; HERPESVIRUS; CANDIDATES; CHALLENGE AB Marek's disease virus (MDV) is an oncogenic alphaherpesvirus and the causative agent of Marek's disease (MD), characterized by immunosuppression, paralysis, nerve enlargement and induction of T-cell lymphomas in chickens. Despite widespread usage of vaccines since the 1970s to control MD, more virulent field strains of MDV have emerged that overcome vaccinal protection, necessitating the development of new and more protective MD vaccines. The increment Meq virus, a recombinant Md5 strain MDV lacking the viral oncogene Meq, is one candidate MD vaccine with great potential but unfortunately it also causes bursal-thymic atrophy (BTA) in maternal antibody negative chickens, raising concerns that impede commercial use as a vaccine. Previously, we identified a point mutation within UL5 that reduced in vivo replication in attenuated viruses. We proposed that introduction of the UL5 point mutation into the increment Meq virus would reduce in vivo replication and eliminate BTA yet potentially retain high protective abilities. In birds, the increment Meq+UL5 recombinant MDV had reduced replication compared to the original increment Meq virus, while weights of lymphoid organs indicated that increment Meq+UL5 did not induce BTA, supporting the hypothesis that reduction of in vivo replication would also abolish BTA. Vaccine trials of the increment Meq+UL5 virus compared to other increment Meq-based viruses and commercial vaccines show that, while the increment Meq+UL5 does provide vaccinal protection, this protection was also reduced compared to the original increment Meq virus. Therefore, it appears that a very delicate balance is required between levels of replication able to induce high vaccinal protection, yet not so high as to induce BTA. C1 [Hildebrandt, Evin] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA. [Hildebrandt, Evin; Dunn, John R.; Cheng, Hans H.] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Cheng, HH (reprint author), USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM hans.cheng@ars.usda.gov FU National Research Initiative Competitive from USDA National Institute of Food and Agriculture [2010-65119-20505] FX This project was supported by National Research Initiative Competitive [grant number 2010-65119-20505] from the USDA National Institute of Food and Agriculture. NR 21 TC 0 Z9 0 U1 2 U2 4 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0307-9457 EI 1465-3338 J9 AVIAN PATHOL JI Avian Pathol. PD JUL 4 PY 2015 VL 44 IS 4 BP 254 EP 258 DI 10.1080/03079457.2015.1041366 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CQ0NO UT WOS:000360293300003 PM 25968878 ER PT J AU Harris, SS Dawson-Hughes, B AF Harris, Susan S. Dawson-Hughes, Bess TI Effects of Hydration and Calcium Supplementation on Urine Calcium Concentration in Healthy Postmenopausal Women SO JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION LA English DT Article DE hydration; calcium supplements; urine calcium concentration; renal stone risk ID KIDNEY-STONE RISK; BODY-WEIGHT; WATER; DISEASE; THIRST; TRIAL; SERUM; DIET; MEN AB Objective: The aim of this study was to determine whether calcium supplementation, compared with placebo, increases urine calcium concentrations to levels indicative of increased renal stone risk, and the role that fluid intake, as indicated by urine volume, may play in mitigating this risk.Methods: This is a secondary analysis of data from a randomized placebo-controlled trial of 500mg/d calcium supplementation to prevent bone loss. Subjects were 240 white postmenopausal women age 40 to 70years in good general health. Effects of supplementation on 1-year changes in 24h urine calcium concentration and urine volume were examined.Results: Both treatment group and urine volume were strong independent predictors of urine calcium concentration (p < 0.001). Among subjects with urine volume under 2 L/24 h, more than half of placebo subjects were at lowest risk for renal stones compared with less than 35% of calcium-supplemented subjects. Among those with higher urine volumes, all placebo subjects and more than 80% of calcium supplemented subjects were at lowest risk.Conclusions: The increased risk of renal stones with calcium supplement use may be largely eliminated with adequate fluid intake, but older adults may not spontaneously consume adequate fluids to minimize this risk and should be counseled to do so. C1 [Harris, Susan S.; Dawson-Hughes, Bess] Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Bone Metab Lab, Boston, MA 02111 USA. RP Harris, SS (reprint author), Tufts Univ, USDA, Jean Mayer HNRCA, 711 Washington St, Boston, MA 02111 USA. EM susan.harris@tufts.edu FU U.S. Department of Agriculture [58-1950-0-014] FX This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 22 TC 0 Z9 0 U1 1 U2 3 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0731-5724 EI 1541-1087 J9 J AM COLL NUTR JI J. Am. Coll. Nutr. PD JUL 4 PY 2015 VL 34 IS 4 BP 340 EP 346 DI 10.1080/07315724.2014.959207 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CP2VP UT WOS:000359735800009 PM 25856469 ER PT J AU Hartati, H Utsunomiya, YT Sonstegard, TS Garcia, JF Jakaria, J Muladno, M AF Hartati, Hartati Utsunomiya, Yuri Tani Sonstegard, Tad Stewart Garcia, Jose Fernando Jakaria, Jakaria Muladno, Muladno TI Evidence of Bos javanicus x Bos indicus hybridization and major QTLs for birth weight in Indonesian Peranakan Ongole cattle SO BMC GENETICS LA English DT Article DE Peranakan Ongole; Nellore; Bos indicus; Bos javanicus; birth weight ID GENOME-WIDE ASSOCIATION; QUANTITATIVE TRAIT LOCI; GENETIC-STRUCTURE; BRAHMAN CATTLE; GROWTH; PLAG1; ZEBU; PREDICTIONS; REGRESSION; EVOLUTION AB Background: Peranakan Ongole (PO) is a major Indonesian Bos indicus breed that derives from animals imported from India in the late 19(th) century. Early imports were followed by hybridization with the Bos javanicus subspecies of cattle. Here, we used genomic data to partition the ancestry components of PO cattle and map loci implicated in birth weight. Results: We found that B. javanicus contributes about 6-7 % to the average breed composition of PO cattle. Only two nearly fixed B. javanicus haplotypes were identified, suggesting that most of the B. javanicus variants are segregating under drift or by the action of balancing selection. The zebu component of the PO genome was estimated to derive from at least two distinct ancestral pools. Additionally, well-known loci underlying body size in other beef cattle breeds, such as the PLAG1 region on chromosome 14, were found to also affect birth weight in PO cattle. Conclusions: This study is the first attempt to characterize PO at the genome level, and contributes evidence of successful, stabilized B. indicus x B. javanicus hybridization. Additionally, previously described loci implicated in body size in worldwide beef cattle breeds also affect birth weight in PO cattle. C1 [Hartati, Hartati] Indonesian Agcy Agr Res & Dev, Minist Agr, Beef Cattle Res Stn, Pasuruan 16784, East Java, Indonesia. [Utsunomiya, Yuri Tani; Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, Brazil. [Sonstegard, Tad Stewart] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, BR-16050680 Sao Paulo, Brazil. [Jakaria, Jakaria; Muladno, Muladno] Bogor Agr Univ, Fac Anim Sci, Bogor 16680, Indonesia. RP Hartati, H (reprint author), Indonesian Agcy Agr Res & Dev, Minist Agr, Beef Cattle Res Stn, Jln Pahlawan 2 Grati, Pasuruan 16784, East Java, Indonesia. EM hartati06@yahoo.com; muladno@gmail.com RI Utsunomiya, Yuri Tani/K-5214-2013 OI Utsunomiya, Yuri Tani/0000-0002-6526-8337 FU Indonesian Agency for Agricultural Research and Development; Ministry of Agriculture through KKP3N activity; Sao Paulo Research Foundation (FAPESP) [2014/01095-8] FX The authors are grateful to the Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture through KKP3N activity in 2013 and 2014 that has facilitated and funded the research activities. YTU is supported by the Sao Paulo Research Foundation (FAPESP) - process 2014/01095-8. Mention of trade name proprietary product in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the authors or their respective institutions. NR 38 TC 1 Z9 1 U1 0 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUL 4 PY 2015 VL 16 AR 75 DI 10.1186/s12863-015-0229-5 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA CM0KG UT WOS:000357366200001 PM 26141727 ER PT J AU Zeng, HW Wu, M AF Zeng, Huawei Wu, Min TI The Inhibitory Efficacy of Methylseleninic Acid Against Colon Cancer Xenografts in C57BL/6 Mice SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID TISSUE SELENIUM LEVELS; HUMAN PROSTATE-CANCER; CELL-PROLIFERATION; COLORECTAL-CANCER; DIETARY SELENIUM; VITAMIN-E; PREVENTION; APOPTOSIS; RATS; SELENOMETHIONINE AB Data indicate that methylselenol is a critical selenium (Se) metabolite for anticancer activity in vivo. We tested the hypothesis that oral dosing methylseleninic acid (MSeA), a methylselenol precursor, inhibits the growth of colon cancer xenografts in C57BL/6 mice fed a Se adequate diet. In this study, MSeA supplementation was given by an oral dose (0, 1, or 3mg/kg body weight) regimen. MSeA increased Se content of liver, kidney, muscle, stomach (w/intestine) and plasma, and elevated blood glutathione peroxidase (GPx) activities. However, MSeA did not change lean/fat body composition, food consumption, levels of plasma leptin/adiponectin, and body weight gain. MSeA (3mg/kg body weight) inhibited tumor growth up to 61% when compared to the control group, and this inhibition was associated with a reduction of plasma tumor necrosis factor (TNF)/interleukin 6 (IL6) level but elevated blood GPx activities. In addition, MSeA (1mg/kg body weight) increased the activation of caspase-3, a major apoptotic enzyme, in tumor tissues. Taken together, our MSeA oral dosing regimen was at safe levels; and high blood GPx activities, caspase-3 activities in tumor tissue and a reduction of plasma TNF/IL6 level, play critical roles in inhibiting colon tumor growth in an immune-competent C57BL/6 mouse model. C1 [Zeng, Huawei] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. [Wu, Min] Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58201 USA. RP Zeng, HW (reprint author), ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. EM huawei.zeng@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service, CRIS project [5450-51000-045-00] FX This work was directly funded by the US Department of Agriculture, Agricultural Research Service, CRIS project (5450-51000-045-00). NR 37 TC 2 Z9 2 U1 3 U2 9 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0163-5581 EI 1532-7914 J9 NUTR CANCER JI Nutr. Cancer PD JUL 4 PY 2015 VL 67 IS 5 BP 831 EP 838 DI 10.1080/01635581.2015.1042547 PG 8 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA CL5SJ UT WOS:000357020600014 PM 26011019 ER PT J AU Williams, MD Goodrick, SL Grundstein, A Shepherd, M AF Williams, Marcus D. Goodrick, Scott L. Grundstein, Andrew Shepherd, Marshall TI Comparison of dew point temperature estimation methods in Southwestern Georgia SO PHYSICAL GEOGRAPHY LA English DT Article DE artificial neural network; dew point temperature; irrigation; land-use change; linear regression ID ARTIFICIAL NEURAL-NETWORKS; MINIMUM TEMPERATURE; LAND-USE; IRRIGATION; CLIMATE; PRECIPITATION; PLAINS; MODELS; IMPACT; MEMORY AB Recent upward trends in acres irrigated have been linked to increasing near-surface moisture. Unfortunately, stations with dew point data for monitoring near-surface moisture are sparse. Thus, models that estimate dew points from more readily observed data sources are useful. Daily average dew temperatures were estimated and evaluated at 14 stations in Southwest Georgia using linear regression models and artificial neural networks (ANN). Estimation methods were drawn from simple and readily available meteorological observations, therefore only temperature and precipitation were considered as input variables. In total, three linear regression models and 27 ANN were analyzed. The two methods were evaluated using root mean square error (RMSE), mean absolute error (MAE), and other model evaluation techniques to assess the skill of the estimation methods. Both methods produced adequate estimates of daily averaged dew point temperatures, with the ANN displaying the best overall skill. The optimal performance of both models was during the warm season. Both methods had higher error associated with colder dew points, potentially due to the lack of observed values at those ranges. On average, the ANN reduced RMSE by 6.86% and MAE by 8.30% when compared to the best performing linear regression model. C1 [Williams, Marcus D.; Goodrick, Scott L.] US Forest Serv, USDA, Ctr Forest Disturbance Sci, Athens, GA 30602 USA. [Williams, Marcus D.; Grundstein, Andrew; Shepherd, Marshall] Univ Georgia, Dept Geog, Athens, GA 30602 USA. RP Williams, MD (reprint author), US Forest Serv, USDA, Ctr Forest Disturbance Sci, Athens, GA 30602 USA. EM mwilliams.cfds@gmail.com FU United States Forest Service; Department of Geography at UGA FX We acknowledge the support of this work by the United States Forest Service and the Department of Geography at UGA. We also thank the support staff of the GAEMN for kindly providing the data for this study and the reviewers for their thoughtful input and feedback. NR 29 TC 0 Z9 0 U1 1 U2 7 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0272-3646 EI 1930-0557 J9 PHYS GEOGR JI Phys. Geogr. PD JUL 4 PY 2015 VL 36 IS 4 BP 255 EP 267 DI 10.1080/02723646.2015.1011554 PG 13 WC Environmental Sciences; Geography, Physical; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Physical Geography; Geology; Meteorology & Atmospheric Sciences GA CL1JV UT WOS:000356699600001 ER PT J AU Chacon, P Lorenz, K Lal, R Calhoun, FG Fausey, NR AF Chacon, Paula Lorenz, Klaus Lal, Rattan Calhoun, Frank G. Fausey, Norman R. TI Association of soil organic carbon with physically separated soil fractions in different land uses of Costa Rica SO ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE LA English DT Article DE soil organic carbon; particulate organic matter; water-stable aggregates; mean weight diameter; secondary particle size fractions; primary particle size fractions ID PARTICLE-SIZE FRACTIONS; TENSILE-STRENGTH; MATTER DYNAMICS; SEQUESTRATION RATES; AGGREGATE HIERARCHY; TROPICAL SOILS; NO-TILLAGE; STABILIZATION; TURNOVER; STABILITY AB Studies on soil organic carbon (SOC) storage and stability require the separate assessment of soil fractions in relation to protection against loss. This on-farm study assessed the role of primary (sand, silt, and clay) and secondary (micro- and macroaggregates) particle size fractions on the physical protection of SOC to 30-cm depth of nine agricultural and three forest land uses within three contrasting ecoregions of Costa Rica (i.e., Isthmian-Atlantic moist, Pacific dry, and Montane forest). Specific objectives were to: (1) determine the effect of land use in each ecoregion on particulate organic matter carbon (POM-C), silt plus clay-associated carbon (S+C-C), and micro- and macroaggregate associated water-stable aggregate carbon (WSA-C), and (2) establish the relationships among aggregate properties and SOC concentration. Aggregate stability was characterized by studying the WSA, mean weight diameter (MWD), and tensile strength (TS). Soils under coffee (Coffea arabica), oil palm (Elaeis guineensis), and pineapple (Ananas comosus) plantations had high clay contents to 30-cm depth (ranging between 42.9 and 60.7%), which indicated a high potential for SOC storage. In comparison to less disturbed forest soils, micro- and macroaggregation was not strongly affected by agricultural land use. At coffee and pasture land uses, POM-C concentration was significantly lower (ranging between 0.4 and 2.0 g C kg(-1)). Between 58 and 98% of SOC was associated with S+C fractions indicating the importance of these particle size fractions to SOC storage. The S+C-C and micro- and macroaggregate-C concentrations differed mainly among pineapple, mango (Mangifera indica), pasture, and other land uses within ecoregions. Further, there was no general tendency of higher C concentrations in the macro- vs. the microaggregate fractions in contrast to the aggregate hierarchy theory. The MWD was affected by land use only for pineapple and organic farming, and it decreased for mango and coffee with increase in soil depth. Correlations among SOC, texture, and TS were site-specific. Silt content was more important than the other particle size fractions in explaining SOC contents. In summary, the amount of physically separated soil fractions and their C storage and stability to 30-cm depth differed mainly among pineapple, mango, pasture, and coffee in specific ecoregions. Well-designed long-term field experiments in Costa Rica are needed to develop management practices for enhancing SOC storage and stabilization in physically separated soil fractions. C1 [Chacon, Paula] Guayas Waterkeeper Assoc Studies Neotrop, Cdla Nueva Kennedy, Guayaquil, Ecuador. [Lorenz, Klaus; Lal, Rattan] Ohio State Univ, Coll Food Agr & Environm Sci, Sch Environm & Nat Resources, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. [Calhoun, Frank G.] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Fausey, Norman R.] ARS, USDA, MWA, Soil Drainage Res Unit, Columbus, OH 43210 USA. RP Lorenz, K (reprint author), Ohio State Univ, Coll Food Agr & Environm Sci, Sch Environm & Nat Resources, Carbon Management & Sequestrat Ctr, 2021 Coffey Rd, Columbus, OH 43210 USA. EM lorenz.59@osu.edu FU CWC - TIE (Climate, Water and Carbon - Targeted Investment in Excellence) Program; EARTH University FX This research was funded by the CWC - TIE (Climate, Water and Carbon - Targeted Investment in Excellence) Program in collaboration with EARTH University. NR 64 TC 0 Z9 0 U1 9 U2 113 PU TAYLOR & FRANCIS AS PI OSLO PA KARL JOHANS GATE 5, NO-0154 OSLO, NORWAY SN 0906-4710 EI 1651-1913 J9 ACTA AGR SCAND B-S P JI Acta Agric. Scand. Sect. B-Soil Plant Sci. PD JUL 4 PY 2015 VL 65 IS 5 BP 448 EP 459 DI 10.1080/09064710.2015.1020859 PG 12 WC Agronomy; Soil Science SC Agriculture GA CF1PL UT WOS:000352319300006 ER PT J AU Summers, CF Adair, NL Gent, DH Mcgrath, MT Smart, CD AF Summers, Carly F. Adair, Nanci L. Gent, David H. Mcgrath, Margaret T. Smart, Christine D. TI Pseudoperonospora cubensis and P. humuli detection using species-specific probes and high definition melt curve analysis SO CANADIAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE cucurbit downy mildew; detection; hop downy mildew; Pseudoperonospora cubensis; Pseudoperonospora humuli ID REAL-TIME PCR; LOCKED NUCLEIC-ACIDS; DOWNY MILDEW; QUANTITATIVE PCR; QUANTIFICATION; SPECIALIZATION; MANAGEMENT; GRAPEVINE; SEQUENCE; DISEASE AB Real-time PCR assays using locked nucleic acid (LNA) probes and high resolution melt (HRM) analysis were developed for molecular differentiation of Pseudoperonospora cubensis and P. humuli, causal agents of cucurbit and hop downy mildew, respectively. The assays were based on a previously identified single nucleotide polymorphism (SNP) in the cytochrome oxidase subunit II (cox2) gene that differentiates the two species. Sequencing of the same region from 15 P. cubensis isolates collected in New York State for the current study confirmed that all isolates shared the conserved SNP. LNA probes were specific and sensitive, detecting as few as 10 sporangia for both species and as little as 1fg P. cubensis total DNA and 10fg P. humuli total DNA. The LNA assay detected both pathogens from air sampled using spore traps placed in vegetable fields and a hop yard during the summers of 2013 and 2014 and correctly diagnosed symptomatic leaf tissue. High resolution melt analysis (HRM) correctly identified all tested isolates as well as those isolates from symptomatic plants collected in the field. The LNA and HRM assays correctly identified both organisms when tested independently in a second laboratory. The results confirm that the LNA and HRM assays developed can provide reliable identification of both species despite the high molecular similarity of the cox2 gene. ResumeDes tests bases sur la PCR en temps reel avec amorces contenant des acides nucleiques bloques (LNA) et une analyse des courbes de fusion a haute resolution (HRM) ont ete concus en vue de la differenciation moleculaire de Pseudoperonospora cubensis et de P. humuli, agents causals du mildiou chez les cucurbitacees et le houblon, respectivement. Les tests etaient bases sur un polymorphisme mononucleotidique (SNP) prealablement identifie dans le gene de la sous-unite II de la cytochrome oxydase (cox2) qui differencie les deux especes. Le sequencage de la meme region de 15 isolats de P. cubensis, collectes dans l'Etat de New York pour l'etude en cours, a confirme que tous les isolats partageaient le SNP conserve. Les amorces de LNA etaient specifiques et sensibles, detectant aussi peu que 10 sporanges pour les deux especes et a peine 1 fg d'ADN total chez P. cubensis et 10 fg d'ADN total chez P. humuli. Le test avec amorces de LNA a permis de detecter les deux agents pathogenes dans l'air echantillonne des pieges a spores places dans des champs de legumes et dans une houblonniere durant les etes de 2013 et de 2014, et de correctement diagnostiquer les tissus foliaires symptomatiques. L'analyse des courbes de fusion a haute resolution a permis d'identifier correctement tous les isolats testes de meme que ceux provenant de plantes symptomatiques collectees sur le terrain. Les tests avec amorces de LNA et bases sur l'analyse des HRM ont permis d'identifier correctement les deux organismes lorsqu'ils ont tete analyses separement dans un deuxieme laboratoire. Les resultats confirment que les tests avec amorces de LNA et ceux bases sur l'analyse des HRM nouvellement concus peuvent permettre d'identifier precisement les deux especes malgre la forte similarite moleculaire du gene de la cox2. C1 [Summers, Carly F.; Smart, Christine D.] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, SIPS, Geneva, NY 14456 USA. [Adair, Nanci L.; Gent, David H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97330 USA. [Adair, Nanci L.; Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. [Mcgrath, Margaret T.] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Long Isl Hort Res & Extens Ctr, SIPS, Riverhead, NY 11901 USA. RP Smart, CD (reprint author), Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, SIPS, Geneva, NY 14456 USA. EM cds14@cornell.edu FU USDA's Organic Research and Extension Initiative (NIFA) [2012-51300-20006]; USDA-ARS CRIS [2072-21000-046-00]; NSF Graduate Research Fellowship [DGE-1144153] FX This work was supported by the USDA's Organic Research and Extension Initiative (NIFA contract no. 2012-51300-20006), and USDA-ARS CRIS 2072-21000-046-00. Funding for C.F. Summers was also provided through an NSF Graduate Research Fellowship (DGE-1144153). NR 47 TC 5 Z9 5 U1 4 U2 10 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0706-0661 EI 1715-2992 J9 CAN J PLANT PATHOL JI Can. J. Plant Pathol. PD JUL 3 PY 2015 VL 37 IS 3 BP 315 EP 330 DI 10.1080/07060661.2015.1053989 PG 16 WC Plant Sciences SC Plant Sciences GA CW4ES UT WOS:000364944700007 ER PT J AU Webb, KM Brenner, T Jacobsen, BJ AF Webb, Kimberly M. Brenner, Tammy Jacobsen, Barry J. TI Temperature effects on the interactions of sugar beet with Fusarium yellows caused by Fusarium oxysporum f. sp betae SO CANADIAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Beta vulgaris; climate change; environmental conditions; resistance screening; sugarbeet; disease development ID ROOT-ROT; SOIL-TEMPERATURE; ENVIRONMENTAL-CONDITIONS; DISEASE RESISTANCE; BIOLOGICAL-CONTROL; RHIZOCTONIA ROOT; CLIMATE-CHANGE; PLANT-DISEASE; 1ST REPORT; MOISTURE AB Fusarium yellows, caused by Fusarium oxysporum f. sp. betae, causes significant yield and storage losses in sugar beet. The pathogen can be highly variable and sugar beet cultivars can have differing responses to fungal infection. Environmental factors may be contributing to this variability in disease response and these interactions have not been fully described. We describe how air temperature may influence interactions of F. oxysporum f. sp. betae with sugar beet. Fusarium yellows was assessed at different temperatures in five sugar beet cultivars to determine how increasing temperatures affect disease development. Generally, temperatures of 24 degrees C or higher led to more disease symptoms, while temperatures of 16 degrees C and lower led to fewer symptoms. Additionally, during resistant interactions, Fusarium yellows development did not increase as temperatures increased, indicating that resistance remained effective at higher temperatures. However, some F. oxysporum f. sp. betae isolates had varying abilities to cause disease at the different temperatures tested, with some isolates able to cause more disease at higher temperatures, while for other isolates, mid-range temperatures were optimum for disease development. To gain insight on disease development at higher temperatures, the growth rate of F. oxysporum isolates (both pathogenic and non-pathogenic to sugar beet) was assessed in vitro at 15 degrees C to 35 degrees C. The greatest growth tended to occur at 25 degrees C; however, growth responses varied between isolates and did not always correlate with a disease response. These results suggest that diversity of the fungal population could influence disease severity in the field at different temperatures. ResumeLa jaunisse fusarienne, causee par Fusarium oxysporum f. sp. betae, cause d'importantes pertes chez la betterave a sucre, et ce, tant de rendement que durant l'entreposage. Le pouvoir de l'agent pathogene peut etre fortement variable et les cultivars de betterave a sucre peuvent reagir differemment a l'infection fongique. Les facteurs environnementaux peuvent contribuer a cette variabilite quant a la reaction a la maladie, et ces interactions n'ont pas encore ete entierement decrites. Nous decrivons comment la temperature peut influencer les interactions entre F. oxysporum f. sp. betae et la betterave a sucre. La jaunisse fusarienne a ete evaluee a differentes temperatures chez cinq cultivars afin de determiner a quel point l'augmentation de la temperature peut influencer le developpement de la maladie. De facon generale, les temperatures de 24 degrees C ou plus ont provoque plus de symptomes, tandis que les temperatures de 16 degrees C et moins en ont provoque moins. En outre, au cours des interactions durant lesquelles la resistance entrait en ligne de compte, le developpement de la jaunisse fusarienne ne s'est pas intensifie a mesure que la temperature augmentait, ce qui indique que la resistance demeurait effective aux temperatures plus elevees. Toutefois, certains isolats de F. oxysporum f. sp. betae pouvaient de diverses facons causer la maladie aux differentes temperatures testees, certains isolats engendrant des taux plus eleves de maladie a des temperatures superieures, tandis que, pour d'autres, les temperatures moyennes suffisaient pour provoquer optimalement le developpement de la maladie. Pour mieux comprendre le developpement de la maladie a des temperatures plus elevees, le taux de croissance d'isolats de F. oxysporum (pathogenes et non pathogenes pour la betterave a sucre) a ete evalue in vitro a des temperatures variant de 15 degrees C a 35 degrees C. La plus forte croissance avait tendance a se produire a 25 degrees C; toutefois, les reactions de croissance variaient d'un isolat a l'autre et ne correspondaient pas toujours a une reaction a la maladie. Ces resultats suggerent que la diversite de la population fongique pourrait influencer la gravite de la maladie au champ, et ce, a differentes temperatures. C1 [Webb, Kimberly M.; Brenner, Tammy] USDA ARS, Sugar Beet Res Unit, Ft Collins, CO 80526 USA. [Jacobsen, Barry J.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. RP Webb, KM (reprint author), USDA ARS, Sugar Beet Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM Kimberly.webb@ars.usda.gov FU Western Sugar Cooperative Joint Research Committee; USDA/NIFA Hatch Project [MONB223] FX Funding for this project was provided by the Western Sugar Cooperative Joint Research Committee and USDA/NIFA Hatch Project #MONB223. Fusarium yellows resistant sugar beet cultivars, SYN563 and SYN68, were provided by Syngenta Seeds-Hilleshog (Longmont, CO). We would like to thank Brett Kuwitzky for his help in performing these experiments. We would also like to thank Mark West, who was consulted on statistical analysis. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 53 TC 0 Z9 0 U1 8 U2 17 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0706-0661 EI 1715-2992 J9 CAN J PLANT PATHOL JI Can. J. Plant Pathol. PD JUL 3 PY 2015 VL 37 IS 3 BP 353 EP 362 DI 10.1080/07060661.2015.1071283 PG 10 WC Plant Sciences SC Plant Sciences GA CW4ES UT WOS:000364944700010 ER PT J AU Jones, SJ Pilkington, SJ Gent, DH Hay, FS Pethybridge, SJ AF Jones, S. J. Pilkington, S. J. Gent, D. H. Hay, F. S. Pethybridge, S. J. TI A polymerase chain reaction assay for ascosporic inoculum of Sclerotinia species SO NEW ZEALAND JOURNAL OF CROP AND HORTICULTURAL SCIENCE LA English DT Article DE ascospores; bean; disease forecasting; Sclerotinia sclerotiorum; white mould ID TIME PCR ASSAY; CARPOGENIC GERMINATION; QUANTITATIVE PCR; WHITE MOLD; STEM ROT; DISEASE; INFECTION; QUANTIFICATION; LETTUCE; MODEL AB A polymerase chain reaction (PCR) assay was developed that amplified a 170-bp fragment of the intergenic spacer region of Sclerotinia sclerotiorum, the cause of white mould. Sensitivity was 10 S. sclerotiorum ascospores per DNA extraction (0.2 ascospores per PCR reaction). The presence of soil did not affect sensitivity at 50, 100 and 500 ascospores/DNA extraction, but reduced sensitivity at 25 and 10 ascospores/DNA extraction by 10% and 30%, respectively. The assay did not amplify DNA of Botrytis cinerea but detected S. minor and S. trifoliorum. Utility of the test for detection of S. sclerotiorum ascospores in bean fields was demonstrated using rotating impaction samplers over two seasons. The use of the test in combination with an impaction sampler may provide benefits in time, sensitivity and specificity compared with visual identification and enumeration of spores from traps only. This system may provide an opportunity to schedule fungicides during periods of inoculum presence for disease management. C1 [Jones, S. J.; Pilkington, S. J.; Hay, F. S.] Univ Tasmania, Tasmanian Inst Agr, Burnie, Tas 7320, Australia. [Gent, D. H.] Oregon State Univ, ARS, USDA, Corvallis, OR 97331 USA. [Pethybridge, S. J.] Cornell Univ, New York Agr Expt Stn, Geneva, NY 14456 USA. RP Pethybridge, SJ (reprint author), Cornell Univ, New York Agr Expt Stn, Geneva, NY 14456 USA. EM sjp277@cornell.edu FU Vegetable Levy; Australian Commonwealth Government [VG07126]; University of Tasmania Visiting Scholar Programme FX This project was facilitated by Horticulture Australia Ltd, in partnership with AUSVEG and funded by the Vegetable Levy with matching funding from the Australian Commonwealth Government (VG07126). Travel of Dr Gent to Australia was partially funded by the University of Tasmania Visiting Scholar Programme. Thanks also to Craig Palmer (Tasmanian Institute of Agriculture) for technical assistance, Garry McNab (Simplot Australia Pty Ltd) and growers for access to fields. NR 28 TC 0 Z9 0 U1 4 U2 14 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0114-0671 EI 1175-8783 J9 NEW ZEAL J CROP HORT JI N. Z. J. Crop Hortic. Sci. PD JUL 3 PY 2015 VL 43 IS 3 BP 233 EP 240 DI 10.1080/01140671.2015.1025083 PG 8 WC Agronomy; Horticulture SC Agriculture GA CQ5DT UT WOS:000360624200008 ER PT J AU Brown, AV Hudson, KA AF Brown, Anne V. Hudson, Karen A. TI Developmental profiling of gene expression in soybean trifoliate leaves and cotyledons SO BMC PLANT BIOLOGY LA English DT Article DE Leaf senescence; Cotyledon senescence; Glycine max; Leaf development ID SENESCENCE-ASSOCIATED GENES; AUXIN RESPONSE ELEMENTS; LIGHT-REGULATED GENE; LEAF SENESCENCE; TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; MOLECULAR ANALYSIS; PLANT SENESCENCE; GLYCINE-MAX; DNA-BINDING AB Background: Immediately following germination, the developing soybean seedling relies on the nutrient reserves stored in the cotyledons to sustain heterotrophic growth. During the seed filling period, developing seeds rely on the transport of nutrients from the trifoliate leaves. In soybean, both cotyledons and leaves develop the capacity for photosynthesis, and subsequently senesce and abscise once their function has ended. Before this occurs, the nutrients they contain are mobilized and transported to other parts of the plant. These processes are carefully orchestrated by genetic regulation throughout the development of the leaf or cotyledon. Results: To identify genes involved in the processes of leaf or cotyledon development and senescence in soybean, we used RNA-seq to profile multiple stages of cotyledon and leaf tissues. Differentially expressed genes between stages of leaf or cotyledon development were determined, major patterns of gene expression were defined, and shared genes were identified. Over 38,000 transcripts were expressed during the course of leaf and cotyledon development. Of those transcripts, 5,000 were expressed in a tissue specific pattern. Of the genes that were differentially expressed between both later stage tissues, 90 % had the same direction of change, suggesting that the mechanisms of senescence are conserved between tissues. Analysis of the enrichment of biological functions within genes sharing common expression profiles highlights the main processes occurring within these defined temporal windows of leaf and cotyledon development. Over 1,000 genes were identified with predicted regulatory functions that may have a role in control of leaf or cotyledon senescence. Conclusions: The process of leaf and cotyledon development can be divided into distinct stages characterized by the expression of specific gene sets. The importance of the WRKY, NAC, and GRAS family transcription factors as major regulators of plant senescence is confirmed for both soybean leaf and cotyledon tissues. These results help validate functional annotation for soybean genes and promoters. C1 [Brown, Anne V.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Hudson, Karen A.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Hudson, KA (reprint author), Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, 915 West State St, W Lafayette, IN 47907 USA. EM kkaczoro@purdue.edu FU USDA-ARS CRIS project [3602 110 007D]; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2010-85117-20607] FX The authors are grateful to Dr. R. Khetani and Faye Zheng for analysis scripts, and the Purdue University Bioinformatics Core Facility for the use of alignment software and computation time. The authors would like to that Dr. M. Hudson for critical reading of the manuscript. This work was funded by USDA-ARS CRIS project number 3602 110 007D and the Agriculture and Food Research Initiative (No. 2010-85117-20607) from the USDA National Institute of Food and Agriculture. Product names are necessary to report factually on available data. However the USDA neither guarantees nor warrants the standard of the product, and use of the names implies no approval of the product to the exclusion of others that may also be suitable. NR 66 TC 7 Z9 7 U1 2 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUL 3 PY 2015 VL 15 AR 169 DI 10.1186/s12870-015-0553-y PG 14 WC Plant Sciences SC Plant Sciences GA CM3DH UT WOS:000357561400001 PM 26149852 ER PT J AU Maragos, CM Busman, M Ma, L Bobell, J AF Maragos, Chris M. Busman, Mark Ma, Liang Bobell, John TI Quantification of patulin in fruit leathers by ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA) SO FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT LA English DT Article DE fruit leather; DART-MS; patulin; ambient ionisation mass spectrometry ID TANDEM MASS-SPECTROMETRY; APPLE JUICE CONCENTRATE; ELECTROSPRAY-IONIZATION; MYCOTOXIN ANALYSIS; ASCORBIC-ACID; DRIED FRUITS; REAL-TIME; DART-MS; PRODUCTS; DEGRADATION AB Patulin is a mycotoxin commonly found in certain fruit and fruit products. For this reason many countries have established regulatory limits pertaining to, in particular, apple juice and apple products. Fruit leathers are produced by dehydrating fruit puree, leaving a sweet product that has a leathery texture. A recent report in the literature described the detection of patulin at substantial levels in fruit leathers. To investigate this further, an ultra-high-performance liquid chromatography-photodiode array (UPLC-PDA) method was developed for the sensitive detection of patulin in fruit leathers. Investigations were also made of the suitability of direct analysis in real time-mass spectrometry (DART-MS) for detection of patulin from the surface of fruit leathers. Results indicated DART-MS was insufficiently sensitive for quantification from the surface of home-style apple leathers, although patulin spiked onto the surface of leather or peel could be detected. The UPLC-PDA method was used to determine the fate of patulin during the preparation of home-made fruit leathers. Interestingly, when a home-style process was used, the patulin was not destroyed, but rather increased in concentration as the puree was dehydrated. The UPLC-PDA method was also used to screen for patulin in commercial fruit leathers. Of the 36 products tested, 14 were above the limit of detection (3.5 mu gkg(-1)) and nine were above the limit of quantification (12 mu gkg(-1)). Positive samples were confirmed by UPLC-MS/MS. Only one sample was found above the US regulatory limit for single-strength apple juice products (50 mu gkg(-1)). These results suggest patulin can be concentrated during preparation and can be found in fruit leathers. The limited survey suggests that patulin is fairly prevalent in such commercial products, but that the levels are usually low. C1 [Maragos, Chris M.; Busman, Mark; Bobell, John] ARS, Bacterial Foodborne Pathogens & Mycol, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Ma, Liang] Southwest Univ, Coll Food Sci, Chongqing, Peoples R China. RP Maragos, CM (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM chris.maragos@ars.usda.gov FU USDA Agricultural Research Service [5010-42000-045-00D]; National Basic Research Program of China [2013CB127803]; National Natural Science Foundation of China [31301476] FX This work was supported by the USDA Agricultural Research Service [project number 5010-42000-045-00D]. L. M. was supported by the National Basic Research Program of China [grant number 2013CB127803] and the National Natural Science Foundation of China [grant number 31301476]. NR 56 TC 2 Z9 2 U1 5 U2 28 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1944-0049 EI 1944-0057 J9 FOOD ADDIT CONTAM A JI Food Addit. Contam. Part A-Chem. PD JUL 3 PY 2015 VL 32 IS 7 BP 1164 EP 1174 DI 10.1080/19440049.2015.1036383 PG 11 WC Chemistry, Applied; Food Science & Technology; Toxicology SC Chemistry; Food Science & Technology; Toxicology GA CK6UK UT WOS:000356363600018 PM 25832782 ER PT J AU Lyu, Q Liao, QH Liu, YL Lan, YB AF Lyu, Qiang Liao, Qiuhong Liu, Yanli Lan, Yubin TI Feasibility of SSC Prediction for Navel Orange Based on Origin Recognition Using NIR Spectroscopy SO INTELLIGENT AUTOMATION AND SOFT COMPUTING LA English DT Article DE Partial least squares regression; NIR spectroscopy; Linear discriminant analysis; Navel orange; Origin recognition; Soluble solids content ID NEAR-INFRARED-SPECTROSCOPY; SOLUBLE-SOLIDS; NONDESTRUCTIVE MEASUREMENT; REFLECTANCE SPECTROSCOPY; INTERNAL QUALITY; SUGAR CONTENT; VIS/NIR-SPECTROSCOPY; SINENSIS L.; DRY-MATTER; FIRMNESS AB Soluble solids content (SSC) is one of most important quality indicators of the navel orange. In order to explore the feasibility of SSC prediction for the navel orange from different origins using near infrared (NIR) spectroscopy, we collected seven groups Newhall navel orange (Citrus sinensis (L) Osb.) samples from seven origins (i.e. Beibei, Fengjie, Leibo, Linhai, Wusheng, Xinfeng, and Yizhang) in China, and all the samples were combined as the eighth group. The difference of the growing environments caused the variation of SSC of oranges from different origins. The partial least squares regression (PLS) models were applied to predict the SSC of its origin samples and other origin samples. The results predicted by origin-model were the best compared to cross-origin-prediction results. So it was necessary to recognize origin as the first steps of SSC prediction. Linear discriminant analysis (LDA) model with the top 18 principle components could recognize the origins of samples with 100% accuracy. The overall results demonstrated that it was feasible that SSC prediction for navel orange based on origin recognition using NIR spectroscopy. C1 [Lyu, Qiang; Liao, Qiuhong; Liu, Yanli] Chinese Acad Agr Sci, Citrus Res Inst, Chongqing, Peoples R China. [Lyu, Qiang] Key Lab Phys Proc Agr Prod Jiangsu, Zhenjiang, Peoples R China. [Lan, Yubin] ARS, USDA, College Stn, TX USA. RP Lyu, Q (reprint author), Chinese Acad Agr Sci, Citrus Res Inst, Chongqing, Peoples R China. EM lvqiang@cric.cn FU Chongqing Science & Technology Commission [cstc2011gjhz80001, cstc2012gg-yyjs80002]; International Science & Technology Cooperation Program of China [2013DFA11470]; Fundamental Research Funds for the Central Universities [XDJK2013C102, XDJK2013A002]; Open Foundation of Agricultural Product Physical Processing Laboratory [JAPP2012-2]; National Sparking Plan Project [2012GA8110017] FX This study was supported by Chongqing Science & Technology Commission (Grant No. cstc2011gjhz80001, and cstc2012gg-yyjs80002), International Science & Technology Cooperation Program of China (2013DFA11470), Fundamental Research Funds for the Central Universities (Grant No. XDJK2013C102, and XDJK2013A002), Open Foundation of Agricultural Product Physical Processing Laboratory (Grant No. JAPP2012-2) and National Sparking Plan Project (Grant No. 2012GA8110017). NR 34 TC 0 Z9 0 U1 3 U2 14 PU AUTOSOFT PRESS PI SAN ANTONIO PA 18015 BOLLIS HILL, SAN ANTONIO, TX 78258 USA SN 1079-8587 EI 2326-005X J9 INTELL AUTOM SOFT CO JI Intell. Autom. Soft Comput. PD JUL 3 PY 2015 VL 21 IS 3 BP 305 EP 317 DI 10.1080/10798587.2015.1015775 PG 13 WC Automation & Control Systems; Computer Science, Artificial Intelligence SC Automation & Control Systems; Computer Science GA CG7CJ UT WOS:000353459500003 ER PT J AU Ranger, CM Schultz, PB Frank, SD Chong, JH Reding, ME AF Ranger, Christopher M. Schultz, Peter B. Frank, Steven D. Chong, Juang H. Reding, Michael E. TI Non-Native Ambrosia Beetles as Opportunistic Exploiters of Living but Weakened Trees SO PLOS ONE LA English DT Article ID ETHANOL-BAITED TRAPS; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; XYLOSANDRUS-GERMANUS COLEOPTERA; BLACK-WALNUT; EXOTIC BARK; STRESS; ATTRACTION; EFFICACY; FUNGUS; PLANTS AB Exotic Xylosandrus spp. ambrosia beetles established in non-native habitats have been associated with sudden and extensive attacks on a diverse range of living trees, but factors driving their shift from dying/dead hosts to living and healthy ones are not well understood. We sought to characterize the role of host physiological condition on preference and colonization by two invaders, Xylosandrus germanus and Xylosandrus crassiusculus. When given free-choice under field conditions among flooded and non-flooded deciduous tree species of varying intolerance to flooding, beetles attacked flood-intolerant tree species over more tolerant species within 3 days of initiating flood stress. In particular, flood-intolerant flowering dogwood (Cornus florida) sustained more attacks than flood-tolerant species, including silver maple (Acer saccharinum) and swamp white oak (Quercus bicolor). Ethanol, a key host-derived attractant, was detected at higher concentrations 3 days after initiating flooding within stems of flood intolerant species compared to tolerant and non-flooded species. A positive correlation was also detected between ethanol concentrations in stem tissue and cumulative ambrosia beetle attacks. When adult X. germanus and X. crassiusculus were confined with no-choice to stems of flood-stressed and non-flooded C. florida, more ejected sawdust resulting from tunneling activity was associated with the flood-stressed trees. Furthermore, living foundresses, eggs, larvae, and pupae were only detected within galleries created in stems of flood-stressed trees. Despite a capability to attack diverse tree genera, X. germanus and X. crassiusculus efficiently distinguished among varying host qualities and preferentially targeted trees based on their intolerance of flood stress. Non-flooded trees were not preferred or successfully colonized. This study demonstrates the host-selection strategy exhibited by X. germanus and X. crassiusculus in non-native habitats involves detection of stress-induced ethanol emission and early colonization of living but weakened trees. C1 [Ranger, Christopher M.; Reding, Michael E.] USDA ARS, Hort Insects Res Lab, Wooster, OH 44691 USA. [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Dept Entomol, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Schultz, Peter B.] Virginia Polytech Inst & State Univ, Hampton Rd Agr Res & Extens Ctr, Virginia Beach, VA USA. [Frank, Steven D.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Chong, Juang H.] Clemson Univ, Pee Dee Res & Educ Ctr, Florence, SC 29506 USA. RP Ranger, CM (reprint author), USDA ARS, Hort Insects Res Lab, Wooster, OH 44691 USA. EM christopher.ranger@ars.usda.gov FU USDA Floriculture and Nursery Research Initiative; USDA ARS Research Project (National Program 304-Crop Protection and Quarantine) [3607-22000-012-00D]; Horticultural Research Institute FX This research was funded by the USDA Floriculture and Nursery Research Initiative and base funds associated with USDA ARS Research Project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine), and the Horticultural Research Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 74 TC 6 Z9 7 U1 3 U2 106 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 2 PY 2015 VL 10 IS 7 AR e0131496 DI 10.1371/journal.pone.0131496 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CN1CS UT WOS:000358154400052 PM 26134522 ER PT J AU Bredeson, MM Lundgren, JG AF Bredeson, Michael M. Lundgren, Jonathan G. TI A Survey of the Foliar and Soil Arthropod Communities in Sunflower (Helianthus annuus) Fields of Central and Eastern South Dakota SO JOURNAL OF THE KANSAS ENTOMOLOGICAL SOCIETY LA English DT Article DE Insect community; Bioinventory; Predators; Pollinators; Herbivores; Detritivores ID NATURAL ENEMIES; INSECT; BIODIVERSITY; AGRICULTURE; CONSERVATION; MANAGEMENT; VEGETATION; ASTERACEAE; HERBIVORY AB The long coevolutionary history between sunflowers (Helianthus annuus, Asterales: Asteraceae) and arthropods in the northern Great Plains has resulted in a commonly grown oilseed crop that harbors a large diversity of insects. A bioinventory of foliar and subterranean arthropods was performed in 22 sunflower fields over a period of three site years in central and eastern South Dakota. Overall, 467 morphospecies were collected. From foliage, 15 arthropod orders were observed. Those containing the greatest species diversity were Hymenoptera, Coleoptera, Hemiptera, Diptera and Araneae with 80; 53; 53; 40 and 30 morphospecies each, respectively. Subterranean arthropods from 19 orders were collected. The five orders containing the highest number of morphospecies were Coleoptera, Hymenoptera, Hemiptera, Araneae and Diptera containing 77; 17; 14; 11 and nine morphospecies respectively. Although bioinventories can be expensive and time consuming, information gathered from them has many uses, including efforts to assess the implications of pesticide use, wildlife conservation, land use- and climate-change on community structure in sunflowers. C1 [Bredeson, Michael M.] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57006 USA. [Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Lundgren, JG (reprint author), USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. EM jonathan.lundgren@ars.usda.gov NR 43 TC 0 Z9 0 U1 4 U2 5 PU KANSAS ENTOMOLOGICAL SOC PI LAWRENCE PA PO BOX 368, LAWRENCE, KS 66044 USA SN 0022-8567 EI 1937-2353 J9 J KANSAS ENTOMOL SOC JI J. Kans. Entomol. Soc. PD JUL PY 2015 VL 88 IS 3 BP 305 EP 315 PG 11 WC Entomology SC Entomology GA DA0QB UT WOS:000367501100001 ER PT J AU Hu, JG Kwon, SJ Park, JJ Landry, E Mattinson, DS Gang, DR AF Hu, Jinguo Kwon, Soon-Jae Park, Jeong-Jin Landry, Erik Mattinson, D. Scott Gang, David R. TI LC-MS determination of L-DOPA concentration in the leaf and flower tissues of six faba bean (Vicia faba L.) lines with common and rare flower colors SO FUNCTIONAL FOODS IN HEALTH AND DISEASE LA English DT Article DE Faba bean; L-DOPA; Parkinson's disease ID PENTOSE-PHOSPHATE PATHWAY; PARKINSONS-DISEASE; ANTIOXIDANT ACTIVITY; STIMULATION; PHENOLICS; CONVICINE; FEATURES; PROLINE; SYSTEMS AB Background: Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the loss of muscle control, which causes trembling of the limbs and head as well as impaired balance. L-DOPA (L-3,4-dihydroxy phenylalanine) is the major ingredient of several prescription drugs used to treat PD. Faba bean (Vicia faba L.) is one of the few plant species that is known to produce L-DOPA and has the potential to be developed as a functional food crop for people suffering with PD. Objective: Aimed to provide needed information for people who want to use faba bean as a natural remedy or functional food to relieve PD symptoms, this study analyzed the variation of L-DOPA concentration in the leaf and flower tissues of six faba bean lines with common and rare flower colors. Methods: Leaf and flower samples were taken from field grown plants with different flower colors, namely, pink with purple lines and black dots, pure white, brown, and crimson. Samples were freeze-dried and L-DOPA was quantified by a LC-MS system consisting of an ACQUITY UPLC in line with a Synapt G2 HDMS quadrupole time-of-flight mass spectrometer. This experiment was carried out in two consecutive years (2012 and 2013) and the plants used in the second year were grown from the seeds harvested from the plants used in the first year. Results and Discussion: Our two-year study revealed a high level of variation in L-DOPA concentration for leaf and flower tissues among the six faba bean lines studied. The average L-DOPA concentration based on dry weight (DW) in flowers ranged from 27.8 to 63.5 mg/g and 18.2 to 48.7 mg/g for leaf tissues. There was no significant correlation between L-DOPA concentrations in flowers and leaves. The L-DOPA concentration in flowers and in leaves of the same line varied but was not statistically significant between the two years. Ideally, the genotype with the highest average L-DOPA concentration in both flowers and leaves would be grown as a natural source of this medicinally important molecule. When developing faba bean as a functional food crop for PD patients, a careful selection of genotype seems necessary for exploiting the full potential of this natural remedy. Conclusions: Faba bean has the potential to be developed as a functional food crop for PD patients. Consumption of young pods and leaves is the most practical means for direct intake or processing of L-DOPA from the faba bean plant. Favorable environmental conditions for growth will optimize L-DOPA yield. Further analysis of the genetic control of L-DOPA synthesis and metabolism will be valuable, with the possibility of developing environmentally resilient cultivars that can produce desirable amounts of L-DOPA for pharmaceutical use. C1 [Hu, Jinguo; Kwon, Soon-Jae; Landry, Erik] Washington State Univ, USDA, ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. [Park, Jeong-Jin; Gang, David R.] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA. [Hu, Jinguo; Landry, Erik] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Mattinson, D. Scott] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Kwon, Soon-Jae] Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jeongeup 580185, Jeonbuk, South Korea. RP Hu, JG (reprint author), Washington State Univ, USDA, ARS, Western Reg Plant Intro Stn, 59 Johnson Hall, Pullman, WA 99164 USA. NR 25 TC 0 Z9 0 U1 0 U2 3 PU FUNCTIONAL FOOD CENTER INC PI RICHARDSON PA 1212 HAMPSHIRE LN, STE 213, RICHARDSON, TX 75080 USA SN 2160-3855 J9 FUNCT FOODS HEALTH D JI Funct. Foods Health Dis. PD JUL PY 2015 VL 5 IS 7 BP 243 EP 250 PG 8 WC Food Science & Technology SC Food Science & Technology GA CY1XD UT WOS:000366200700001 ER PT J AU Bostian, M Whittaker, G Barnhart, B Fare, R Grosskopf, S AF Bostian, Moriah Whittaker, Gerald Barnhart, Brad Faere, Rolf Grosskopf, Shawna TI Valuing water quality tradeoffs at different spatial scales: An integrated approach using bilevel optimization SO WATER RESOURCES AND ECONOMICS LA English DT Article DE Integrated modeling; Nonpoint pollution; Bilevel multiobjective optimization; Tradeoff analysis; Conservation targeting ID NONPOINT POLLUTION-CONTROL; POINT-SOURCE POLLUTION; RIVER-BASIN; EVOLUTIONARY ALGORITHMS; IRRIGATED AGRICULTURE; AUTOMATIC CALIBRATION; MANAGEMENT; MODEL; CONSERVATION; LAND AB This study evaluates the tradeoff between agricultural production and water quality at both the watershed scale and the farm scale, using an integrated economic-biophysical hybrid genetic algorithm. We apply a multi-input, multi-output profit maximization model to detailed farm-level production data from the Oregon Willamette Valley to predict each producer's response to a targeted fertilizer tax policy. Their resulting production decisions are included in a biophysical model of basin-level soil and water quality. Building on a general regulation problem for nonpoint pollution, we use a hybrid genetic algorithm to integrate the economic and biophysical models into one bilevel multiobjective optimization problem, the joint maximization of farm profits and minimization of Nitrate runoff resulting from fertilizer usage. This approach allows us to more fully endogenize fertilizer reduction cost, rather than assume an average cost relationship. The solution set of tax rates generates the Pareto optimal frontier at the watershed level. We then measure the tradeoffs between maximum profit and Nitrogen loading for individual farms, subject to the solution fertilizer tax policy. We find considerable variation in tradeoff values across the basin, which could be used to target incentives for reducing Nitrogen loading to agricultural producers under non-uniform control strategies. (C) 2015 Elsevier B.V. All rights reserved. C1 [Bostian, Moriah] Lewis & Clark Coll, Dept Econ, Portland, OR 97219 USA. [Whittaker, Gerald; Barnhart, Brad] ARS, USDA, Corvallis, OR USA. [Faere, Rolf; Grosskopf, Shawna] Oregon State Univ, Dept Econ, Corvallis, OR 97331 USA. RP Bostian, M (reprint author), Lewis & Clark Coll, Dept Econ, Portland, OR 97219 USA. EM mbbostian@lclark.edu NR 75 TC 3 Z9 3 U1 3 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-4284 J9 WATER RESOUR ECON JI Water Resour. Econ. PD JUL PY 2015 VL 11 BP 1 EP 12 DI 10.1016/j.wre.2015.06.002 PG 12 WC Water Resources SC Water Resources GA CY0ZC UT WOS:000366134500001 ER PT J AU Stella, E Moscetti, R Massantini, R Monarca, D Cecchinii, M Haff, RP Contini, M AF Stella, E. Moscetti, R. Massantini, R. Monarca, D. Cecchinii, M. Haff, R. P. Contini, M. TI Recent advances in the use of NIR spectroscopy for qualitative control and protection of extra virgin olive oil SO RIVISTA ITALIANA DELLE SOSTANZE GRASSE LA Italian DT Article DE extra virgin olive oil; near infrared spectroscopy; chemometrics; olive oil quality; olive oil adulterations ID NEAR-INFRARED SPECTROSCOPY; CLASS-MODELING TECHNIQUES; OXIDATIVE STABILITY; FT-NIR; ADULTERATION; PARAMETERS; SPECTRA; AUTHENTICATION; CLASSIFICATION; SPECTROMETRY AB Recent studies on the use of near infrared (NIR) spectroscopy for the qualitative characterization of extra virgin olive oil, are reported and discussed in this paper. Research results confirms that NIR spectroscopy, combined with chemometric data analysis, allows to simultaneously evaluate all qualitative aspects of oil: analytical parameters established by law, chemical composition, sensorial, health and nutritional properties, shelf-life estimation, varietal and geographic identity, presence of adulterants. NIR spectroscopy is a powerful and versatile tool for detecting extra virgin olive oils, being fast, noninvasive, economical and environment friendly. Today, NIR spectroscopy reliability means that it can substitute and/or support traditional analytical techniques, both to monitor the effects of processing on the oil quality and for check purposes. C1 [Stella, E.; Monarca, D.; Cecchinii, M.] Univ Tuscia, Dipartimento Sci & Tecnol Agr Foreste Nat & Energ, Viterbo, Italy. [Moscetti, R.; Massantini, R.; Contini, M.] Univ Tuscia, Dipartimento Innovaz Sistemi Biol Agroalimentari, Viterbo, Italy. [Haff, R. P.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. RP Contini, M (reprint author), Univ Tuscia, Dipartimento Innovaz Sistemi Biol Agroalimentari, Viterbo, Italy. EM mcontini@unitus.it NR 54 TC 2 Z9 2 U1 7 U2 19 PU SERVIZI EDITORIALI ASSOC SRL PI COMO PA VIA ADAMO DEL PERO, 6, COMO, 22100, ITALY SN 0035-6808 J9 RIV ITAL SOSTANZE GR JI Riv. Ital. Sostanze Grasse PD JUL-SEP PY 2015 VL 92 IS 3 BP 155 EP 173 PG 19 WC Food Science & Technology SC Food Science & Technology GA CX1NF UT WOS:000365462200001 ER PT J AU Diaz, JAC Vallet, JL Lents, CA Nonneman, DJ Miles, JR Wright, EC Rempel, LA Cushman, RA Freking, BA Rohrer, GA Phillips, C DeDecker, A Foxcroft, G Stalder, K AF Calderon Diaz, J. A. Vallet, J. L. Lents, C. A. Nonneman, D. J. Miles, J. R. Wright, E. C. Rempel, L. A. Cushman, R. A. Freking, B. A. Rohrer, G. A. Phillips, C. DeDecker, A. Foxcroft, G. Stalder, K. TI Age at puberty, ovulation rate, and uterine length of developing gilts fed two lysine and three metabolizable energy concentrations from 100 to 260 d of age SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE age at puberty; gilts; metabolizable energy; reproductive tract; SID lysine ID REPRODUCTIVE DEVELOPMENT; BODY-COMPOSITION; DIETARY ENERGY; AMINO-ACIDS; GROWTH; SOWS; 1ST; PERFORMANCE; TRAITS AB The objective of this study was to determine the effect of ad libitum feeding diets differing in standard ileal digestible (SID) lysine and ME concentrations that bracket those fed to developing gilts in U.S. commercial settings. Average SID lysine and ME concentrations in diets currently fed to developing gilts were obtained from a poll of the U.S. commercial swine industry. Crossbred Large White. Landrace gilts (n = 1,221), housed in groups, were randomly allotted to 6 corn-soybean diets in a 2 x 3 factorial arrangement formulated to provided 2 SID lysine and 3 ME concentrations. Gilts received grower diets formulated to provide 1.02% (control = survey average) or 0.86% (control minus 15%) SID lysine and 2.94, 3.25, or 3.57 (survey average ME +/- 10%) Mcal of ME/kg from 100 d of age until approximately 90 kg BW. Then, gilts were fed finisher diet containing 0.85% (control = survey average) or 0.73% (control minus 15%) SID lysine and 2.94, 3.26, or 3.59 (control +/- 10%) Mcal of ME/kg until 260 d of age. Gilts were weighed, and backfat thickness and loin muscle area were recorded at the beginning of the trial and then every 28 d. Starting at 160 d of age, gilts were exposed daily to vasectomized boars and observed for behavioral estrus. At approximately 260 d of age, gilts were slaughtered and their reproductive tract was collected. Each reproductive tract was examined to determine whether the gilt was cyclic, the stage of estrus cycle, ovulation rate, and uterine length. Data were evaluated for normality and analyzed using mixed model methods. Average age at puberty was 193 d of age with a range from 160 to 265 d. When all gilts on trial at 160 d of age were included in the analysis, 91.0% reached puberty as determine by observation of standing estrus. Differences between dietary treatments on age at puberty or measurements of the reproductive tract were not detected. Growth rates to 160 d were not limiting for attainment of puberty in response to daily boar stimulation from 160 d. C1 [Calderon Diaz, J. A.; Stalder, K.] Iowa State Univ, Anim Sci, Ames, IA 50011 USA. [Vallet, J. L.; Lents, C. A.; Nonneman, D. J.; Miles, J. R.; Wright, E. C.; Rempel, L. A.; Cushman, R. A.; Freking, B. A.; Rohrer, G. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Phillips, C.; DeDecker, A.] Murphy Brown LLC, Prod Res, Rose Hill, NC 28458 USA. [Foxcroft, G.] Univ Alberta, Dept Swine Reprod Physiol, Edmonton, AB T6G 2P5, Canada. RP Stalder, K (reprint author), Iowa State Univ, Anim Sci, 109 Kildee Hall, Ames, IA 50011 USA. EM stalder@iastate.edu OI Stalder, Kenneth/0000-0001-9540-681X FU National Pork Checkoff under the Sow Lifetime Productivity Project [12-209]; USDA's TARGET Center [(202) 720-2600] FX This work was funded by the National Pork Checkoff under the Sow Lifetime Productivity Project 12-209. The authors would like to thank Murphy Brown LLC for their collaboration during this project. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S. W., Washington, DC. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 23 TC 1 Z9 1 U1 1 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2015 VL 93 IS 7 BP 3521 EP 3527 DI 10.2527/jas.2014-8522 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CW7IB UT WOS:000365170500030 ER PT J AU Pfister, JA Davis, TZ Hall, JO Stegelmeier, BL Panter, KE AF Pfister, J. A. Davis, T. Z. Hall, J. O. Stegelmeier, B. L. Panter, K. E. TI Elk (Cervus canadensis) preference for feeds varying in selenium concentration SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE diet selection; elk; preference; selenium ID FOOD PREFERENCES; PLANTS; SHEEP; FLAVOR; TASTE; ODOR; ACQUISITION; EXPERIENCE; AVERSIONS; TOXICOSIS AB Selenium-accumulator plants are reputed to be unpalatable to large ungulates. Elk (Cervus canadensis) populations in southeastern Idaho overlap with populations of Se-rich plants, but there is no information on the influence of plant Se concentration on elk dietary preferences. The objective of this study was to determine, under controlled conditions, the preference of elk for feeds varying in Se concentrations. Seven yearling female elk (128 +/- 5 kg) were purchased from a commercial elk farm in southeastern Idaho and adapted to low-Se alfalfa pellets. Three feeding trials using pellets with predetermined Se concentrations were conducted. Alfalfa pellets were commercially made with the addition of Symphyotrichum ascendens (western aster) so that the pellets contained 0.4, 5, 20, 50, or 100 mg/kg (DM basis) Se. In trial 1, 5 Se-containing alfalfa pellets (0.4, 5, 20, 50, and 100 mg/kg Se) were offered for 10 d; trial 2 used 4 Se-containing alfalfa pellet choices (0.4, 20, 50, and 100 mg/kg), and trial 3 used 3 pellet choices (0.4, 50, and 100 mg/kg) for 6 d. In trial 1, consumption of the control pellets by elk was greater than each of the other pellet choices (P < 0.001). Similarly, consumption of the 5-mg/kg Se pellet differed from control pellet and all other Se-containing pellets (P < 0.0001). There were no differences (P > 0.50) in consumption of the 20-, 50-, or 100-mg/kg Se pellets. In trial 2, elk consumed more (P < 0.0001) of the control pellet than the 20-, 50-, and 100-mg/kg Se pellets. Similarly, elk consumed more (P < 0.0001) of the 20-mg/kg Se pellet than the 50- and 100-mg/kg Se pellets. There were no differences (P > 0.99) in elk consumption of the 50-and 100-mg/kg Se pellets. In trial 3, elk consumption of the control and 50-and 100-mg/kg Se pellets differed (P <= 0.03) from one another each day except that on d 1 and 2, where elk consumption of the 50-and 100-mg/kg Se pellets did not differ (P >= 0.32). Elk clearly discriminated against pellets with the highest Se concentrations when they were given pellets with differing Se concentrations. These results suggest that elk are not likely to select forages with high Se concentrations, particularly when high-Se plants are present in a rangeland situation with numerous other forage choices. C1 [Pfister, J. A.; Davis, T. Z.; Stegelmeier, B. L.; Panter, K. E.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. [Hall, J. O.] Utah State Vet Diagnost Lab, Logan, UT 84341 USA. RP Pfister, JA (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM jim.pfister@ars.usda.gov NR 59 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2015 VL 93 IS 7 BP 3690 EP 3697 DI 10.2527/jas.2015-9008 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CW7IB UT WOS:000365170500045 PM 26440035 ER PT J AU Buttrey, EK Luebbe, MK McCollum, FT Cole, NA MacDonald, JC Hales, KE AF Buttrey, E. K. Luebbe, M. K. McCollum, F. T., III Cole, N. A. MacDonald, J. C. Hales, K. E. TI Effects of glycerin concentration in steam-flaked corn-based diets with supplemental yellow grease on performance and carcass characteristics of finishing beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; carcass quality; glycerin; yellow grease ID CRUDE GLYCERIN; FERMENTATION CHARACTERISTICS; NUTRIENT DIGESTIBILITY; FEEDLOT CATTLE; LAMBS; FAT AB Forty-eight individually fed crossbred steers (initial BW = 381 +/- 7.61 kg) were used to determine the effects of glycerin (GLY) concentration in steam-flaked corn (SFC)-based diets with added yellow grease on animal performance and carcass characteristics. Glycerin was included at 0, 2.5, 5, and 10% dietary DM replacing SFC. A completely randomized design was used with steers as the experimental unit, and the model included the fixed effects of diet. Contrast statements were used to determine linear and quadratic effects of GLY inclusion. Final BW and DMI were not affected (P > 0.27) by GLY concentration. Average daily gain and G: F based on live BW did not differ as GLY level increased in the diet from 0 to 10% of DM (P > 0.33). Carcass-adjusted final BW and carcass-adjusted G: F were also not affected by GLY concentration (P > 0.22); however, carcass-adjusted ADG tended to respond quadratically by decreasing from 2.5 to 5% GLY inclusion and increasing thereafter (P = 0.10). Calculated dietary NEm and NEg did not differ as GLY increased in the diet (P > 0.37). Hot carcass weight tended to respond quadratically, decreasing from 2.5 to 5% GLY and increasing thereafter (P = 0.10). Likewise, dressing percentage tended to respond quadratically by decreasing from 2.5 to 5% GLY inclusion and increasing to 10% GLY inclusion (P = 0.09). Fat thickness decreased linearly as GLY inclusion increased in the diet (P < 0.01); however, no differences were detected in marbling score and LM area (P > 0.21). Calculated yield grade (YG) decreased linearly as GLY increased in the diet from 0 to 10% of DM (P = 0.04). Based on our results, there was no animal performance benefit for replacing SFC with GLY in diets containing yellow grease, and the only change in carcass merit was a slight improvement in YG. C1 [Buttrey, E. K.; MacDonald, J. C.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX 79016 USA. [Luebbe, M. K.; McCollum, F. T., III; MacDonald, J. C.] Texas AgriLife Res & Extens Ctr, Amarillo, TX 79106 USA. [Cole, N. A.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Hales, K. E.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Hales, KE (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM kristin.hales@ars.usda.gov FU Texas Food and Fiber Commission, Austin FX This research was supported by a grant from the Texas Food and Fiber Commission, Austin. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. The authors have no conflicts of interest. NR 23 TC 1 Z9 1 U1 0 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2015 VL 93 IS 7 BP 3698 EP 3703 DI 10.2527/jas.2015-9138 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CW7IB UT WOS:000365170500046 PM 26440036 ER PT J AU Bateman, C Kendra, PE Rabaglia, R Hulcr, J AF Bateman, Craig Kendra, Paul E. Rabaglia, Robert Hulcr, Jiri TI Fungal symbionts in three exotic ambrosia beetles, Xylosandrus amputatus, Xyleborinus andrewesi, and Dryoxylon onoharaense (Coleoptera: Curculionidae: Scolytinae: Xyleborini) in Florida SO SYMBIOSIS LA English DT Article DE Xyleborini; Ambrosia fungi; Ambrosiella; Lateral transfer; non-native symbiosis ID SOUTHERN PINE-BEETLE; MAXIMUM-LIKELIHOOD; BARK BEETLES; SP NOV.; PHYLOGENY; RAFFAELEA; GLABRATUS; GENUS; EMERGENCE; DYNAMICS AB In nearly every forest habitat, ambrosia beetles (Coleoptera: Curculionidae: Scolytinae, Platypodinae) plant and maintain symbiotic fungus gardens inside dead or dying trees. Some non-native ambrosia beetles aggressively attack live trees and damage tree crops, lumber, and native woody plant taxa by introducing ambrosia fungi, some of which are plant pathogens. Most established exotic species, however, do not cause any economic damage, and consequently are little studied. To determine the specificity and diversity of ambrosia symbionts in under-studied non-native beetles in Florida, fungi were isolated from three species: Xylosandrus amputatus, Xyleborinus andrewesi, and Dryoxylon onoharaense. Two of the beetles sampled each yielded a fungal species isolated with 100 % frequency: X. amputatus: Ambrosiella beaveri or A. nakashimae, and X. andrewesi: Raffaelea sp. nov. nr. canadensis. Both of these symbionts have been isolated previously from closely related ambrosia beetles, supporting the hypothesis that some beetles can carry monocultures of fungi, but the fungi may not be specific to single beetle species. No consistent fungi were isolated from Dryoxylon onoharaense, raising questions about whether or not it truly carries its own symbionts. These results are now being used to test hypotheses and models explaining the evolution of pathogenicity within ambrosia fungi and invasion ability within exotic beetle-fungus complexes. C1 [Bateman, Craig; Hulcr, Jiri] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Kendra, Paul E.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Rabaglia, Robert] US Forest Serv, USDA, Forest Hlth Protect, Washington, DC 20250 USA. [Hulcr, Jiri] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. RP Hulcr, J (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM hulcr@ufl.edu FU United States Department of Agriculture (USDA) Forest Service (FS)-SRS [14-CA-11,330,130-032]; USDA-FS-FHP [12-CA-11,420,004-042]; USDA [14-8130-0377-CA]; National Science Foundation [DEB 1256968]; University of Florida Opportunity Seed fund FX This study was funded by United States Department of Agriculture (USDA) Forest Service (FS)-SRS Coop agreement 14-CA-11,330,130-032, USDA-FS-FHP Coop agreement 12-CA-11,420,004-042, USDA Farm Bill agreement 14-8130-0377-CA, National Science Foundation DEB 1256968, and a University of Florida Opportunity Seed fund. NR 46 TC 2 Z9 2 U1 3 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0334-5114 EI 1878-7665 J9 SYMBIOSIS JI Symbiosis PD JUL PY 2015 VL 66 IS 3 BP 141 EP 148 DI 10.1007/s13199-015-0353-z PG 8 WC Microbiology SC Microbiology GA CX2HG UT WOS:000365517100004 ER PT J AU Eschbaumer, M Li, WS Wernike, K Marshall, F Czub, M AF Eschbaumer, Michael Li, Wansi (May) Wernike, Kerstin Marshall, Frank Czub, Markus TI Probe-free real-time reverse transcription polymerase chain reaction assays for the detection and typing of porcine reproductive and respiratory syndrome virus in Canada SO CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE LA English DT Article ID MEDIATED ISOTHERMAL AMPLIFICATION; MELTING CURVE ANALYSIS; RT-PCR ASSAYS; RAPID DETECTION; SYBR-GREEN; MULTIPLEX PCR; MOLECULAR DIAGNOSTICS; CIRCOVIRUS TYPE-2; NORTH-AMERICAN; AMPLICON SIZE AB Porcine reproductive and respiratory syndrome (PRRS) has tremendous impact on the pork industry in North America. The molecular diagnosis of infection with PRRS virus (PRRSV) is hampered by its considerable strain diversity. In this study, 43 previously published or newly developed primers for probe-free real-time reverse transcription polymerase chain reaction (RT-PCR) were evaluated on their sensitivity, specificity, reproducibility, and repeatability, using a diverse panel of 36 PRRSV strains as well as other arteriviruses and unrelated porcine viruses. Three primer pairs had excellent diagnostic and analytical sensitivity on par with a probe-based reference assay, absolute specificity to virus genotype and species, as well as over 95% reproducibility and repeatability across a wide dynamic range. C1 [Eschbaumer, Michael; Li, Wansi (May); Czub, Markus] Univ Calgary, Fac Vet Med, Calgary, AB, Canada. [Wernike, Kerstin] Friedrich Loeffler Inst, Greifswald, Germany. [Marshall, Frank] Marshall Swine & Poultry Hlth Serv, Camrose, AB, Canada. RP Eschbaumer, M (reprint author), USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM michael.eschbaumer@ars.usda.gov FU Alberta Livestock and Meat Agency Ltd. [2012R033R] FX This study was funded by the Alberta Livestock and Meat Agency Ltd. as part of a pilot project assessing the pen-side molecular diagnosis of PCV2 and PRRSV (project number 2012R033R). NR 45 TC 0 Z9 0 U1 1 U2 4 PU CANADIAN VET MED ASSOC PI OTTAWA PA 339 BOOTH ST, OTTAWA, ONTARIO K1R 7K1, CANADA SN 0830-9000 J9 CAN J VET RES JI Can. J. Vet. Res.-Rev. Can. Rech. Vet. PD JUL PY 2015 VL 79 IS 3 BP 170 EP 179 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA CW4MG UT WOS:000364965000002 PM 26130848 ER PT J AU Springsteen, B Christofk, T York, RA Mason, T Baker, S Lincoln, E Hartsough, B Yoshioka, T AF Springsteen, Bruce Christofk, Thomas York, Robert A. Mason, Tad Baker, Stephen Lincoln, Emily Hartsough, Bruce Yoshioka, Takuyuki TI Forest biomass diversion in the Sierra Nevada: Energy, economics and emissions SO CALIFORNIA AGRICULTURE LA English DT Article ID FUELS AB As an alternative to open pile burning, use of forest wastes from fuel hazard reduction projects at Blodgett Forest Research Station for electricity production was shown to produce energy and emission benefits: energy (diesel fuel) expended for processing and transport was 2.5% of the biomass fuel (energy equivalent); based on measurements from a large pile burn, air emissions reductions were 98%-99% for PM2.5, CO (carbon monoxide), NMOC (nonmethane organic compounds), CH4 (methane) and BC (black carbon), and 20% for NOx and CO2-equivalent greenhouse gases. Due to transport challenges and delays, delivered cost was $70 per bone dry ton (BDT) - comprised of collection and processing ($34/BDT) and transport ($36/BDT) for 79 miles one way - which exceeded the biomass plant gate price of $45/BDT. Under typical conditions, the break-even haul distance would be approximately 30 miles one way, with a collection and processing cost of $30/BDT and a transport cost of $16/BDT. Revenue generated from monetization of the reductions in air emissions has the potential to make forest fuel reduction projects more economically viable. C1 [Springsteen, Bruce; Christofk, Thomas] Placer Cty Air Pollut Control Dist, Auburn, CA 95603 USA. [York, Robert A.] UC Ctr Forestry, Berkeley, CA USA. [York, Robert A.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA USA. [Mason, Tad] TSS Consultants, Rancho Cordova, CA USA. [Baker, Stephen; Lincoln, Emily] Missoula Fire Lab, USFS Rocky Mt Res Stn, Missoula, MT USA. [Hartsough, Bruce; Yoshioka, Takuyuki] Univ Calif Davis, Biol & Agr Engn, Davis, CA USA. RP Springsteen, B (reprint author), Placer Cty Air Pollut Control Dist, Auburn, CA 95603 USA. NR 18 TC 0 Z9 0 U1 4 U2 5 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 EI 2160-8091 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2015 VL 69 IS 3 BP 142 EP 149 DI 10.3733/ca.v069n03p142 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA CV8IU UT WOS:000364527900007 ER PT J AU Thompson, MP Anderson, NM AF Thompson, Matthew P. Anderson, Nathaniel M. TI Modeling fuel treatment impacts on fire suppression cost savings: A review SO CALIFORNIA AGRICULTURE LA English DT Review ID REDUCTION TREATMENTS; FOREST RESTORATION; DECISION-MAKING; WILDLAND FIRES; UNITED-STATES; WILDFIRE; BIOMASS; ENERGY; RISK; MANAGEMENT AB High up-front costs and uncertain return on investment make it difficult for land managers to economically justify large-scale fuel treatments, which remove trees and other vegetation to improve conditions for fire control, reduce the likelihood of ignition, or reduce potential damage from wildland fire if it occurs. In the short-term, revenue from harvested forest products can offset treatment costs and broaden opportunities for treatment implementation. Increasingly, financial analysis of fuel treatments is also incorporating long-term savings through reduced fire suppression costs, which can be difficult to quantify. This paper reviews the findings and lessons from recent modeling work evaluating the potential relationship between fuel treatments and avoided fire suppression costs. Across studies, treatments are generally predicted to reduce future fire suppression costs, although the magnitude of savings is unlikely to fully offset fuel treatment costs. This funding gap highlights the importance of forest product revenues in facilitating landscape-scale treatment. Factors influencing the effects of fuel treatment investments on fire suppression costs include the causal pathway linking treatment inputs to suppression cost outcomes; the spatiotemporal uncertainty of wild-fire-treatment interactions; and the scale of fuel treatment programs. C1 [Thompson, Matthew P.; Anderson, Nathaniel M.] US Forest Serv Rocky Mt Res Stn, Human Dimens Program, Missoula, MT 59801 USA. RP Thompson, MP (reprint author), US Forest Serv Rocky Mt Res Stn, Human Dimens Program, Missoula, MT 59801 USA. FU Rocky Mountain Research Station; USDA National Institute of Food and Agriculture; Joint Fire Science Program; National Fire Decision Support Center FX The Rocky Mountain Research Station, the USDA National Institute of Food and Agriculture, the Joint Fire Science Program, and the National Fire Decision Support Center supported this effort. NR 46 TC 1 Z9 1 U1 6 U2 12 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 EI 2160-8091 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2015 VL 69 IS 3 BP 164 EP 170 DI 10.3733/ca.v069n03p164 PG 7 WC Agriculture, Multidisciplinary SC Agriculture GA CV8IU UT WOS:000364527900010 ER PT J AU Rodgers, J Fortier, C Delhom, C Cui, XL AF Rodgers, James Fortier, Chanel Delhom, Chris Cui, Xiaoliang TI Laboratory Ginning and Blending Impacts on Cotton Fiber Micronaire Measurements SO AATCC JOURNAL OF RESEARCH LA English DT Article DE Blending; Cotton; Fiber; Fibronaire; HVI; Laboratory Ginning; Micronaire; Near Infrared; NIR AB For boll or small quantity seed cotton samples, fiber samples can be prepared by laboratory ginning methods, such as hand ginning (HG), laboratory-scale saw ginning (SG), and roller ginning (RG). Fiber blending is sometimes performed, which can impact fiber surface appearance. Interest was expressed on the impact of blending and laboratory ginning methods on Uster High Volume Instrument (HVI), Fibronaire, and near infrared (NIR) micronaire measurements. Blending impacts were slight but discernable by NIR only. Very good lint yield agreement was observed between the ginning methods (42-45%). Laboratory ginning impacts on the measured micronaire results were small, with between measurement method and between gin method agreements established. Thus, the use of any of the evaluated gin methods would yield acceptable and comparable micronaire results. C1 [Rodgers, James] ARS, Cotton Struct & Qual CSQ Res Unit, SRRC, USDA, New Orleans, LA 70124 USA. [Fortier, Chanel; Delhom, Chris; Cui, Xiaoliang] ARS, USDA, SRRC, New Orleans, LA 70124 USA. RP Rodgers, J (reprint author), ARS, USDA, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM james.rodgers@ars.usda.gov NR 11 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC TEXTILE CHEMISTS COLORISTS PI RES TRIANGLE PK PA PO BOX 12215, RES TRIANGLE PK, NC 27709 USA SN 2330-5517 J9 AATCC J RES JI AATCC J. Res. PD JUL-AUG PY 2015 VL 2 IS 4 BP 1 EP 7 PG 7 WC Materials Science, Textiles SC Materials Science GA CU6EA UT WOS:000363623200001 ER PT J AU Ramirez, M Camprubi-Robles, M Barranco, A Lieblein-Boff, JC Kuchan, MJ Lai, CS Johnson, EJ AF Ramirez, M. Camprubi-Robles, M. Barranco, A. Lieblein-Boff, J. C. Kuchan, M. J. Lai, C. S. Johnson, E. J. TI CAROTENOID ACCRETION IN NEONATAL RATS AND PIGLETS SO EUROPEAN JOURNAL OF OPHTHALMOLOGY LA English DT Meeting Abstract C1 [Ramirez, M.; Barranco, A.; Lai, C. S.] Abbott Nutr R&D, Granada, Spain. [Camprubi-Robles, M.] Abbott Nutr R&D, Granada, Spain. [Lieblein-Boff, J. C.; Kuchan, M. J.] Abbott Nutr R&D, Columbus, OH USA. [Johnson, E. J.] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WICHTIG PUBL PI MILAN PA 72/74 VIA FRIULI, 20135 MILAN, ITALY SN 1120-6721 EI 1724-6016 J9 EUR J OPHTHALMOL JI Eur. J. Ophthalmol. PD JUL-AUG PY 2015 VL 25 IS 4 MA MC015 BP E62 EP E62 PG 1 WC Ophthalmology SC Ophthalmology GA CT8EH UT WOS:000363047300026 ER PT J AU Yoon, SC Shin, TS Lawrence, KC Heitschmidt, GW Park, B Gamble, GR AF Yoon, Seung-Chul Shin, Tae-Sung Lawrence, Kurt C. Heitschmidt, Gerald W. Park, Bosoon Gamble, Gary R. TI Hyperspectral imaging using RGB color for foodborne pathogen detection SO JOURNAL OF ELECTRONIC IMAGING LA English DT Article DE hyperspectral image reconstruction; color; hyperspectral imaging; regression; non-O157 Shiga toxin-producing Escherichia coli; foodborne pathogen; pathogen detection ID SPECTRAL REFLECTANCE; SEROGROUPS; SYSTEM AB This paper reports the development of a spectral reconstruction technique for predicting hyperspectral images from RGB color images and classifying food-borne pathogens in agar plates using reconstructed hyperspectral images. The six representative non-O157 Shiga-toxin producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) grown on Rainbow agar plates were used for the study. A line-scan pushbroom hyperspectral imaging spectrometer was used to scan full reflectance spectra of pure non-O157 STEC cultures in the visible and near-infrared spectral range from 400 to 1000 nm. RGB color images were generated by simulation from hyperspectral images. Polynomial multivariate least-squares regression analysis was used to reconstruct hyperspectral images from RGB color images. The mean R-squared value for hyperspectral image reconstruction was similar to 0.98 in the spectral range between 400 and 700 nm for linear, quadratic, and cubic polynomial regression models. The accuracy of the hyperspectral image classification algorithm based on k-nearest neighbors algorithm of principal component scores was validated to be 92% with the test set (99% with the original hyperspectral images). The results of the study suggested that color-based hyperspectral imaging would be feasible without much loss of prediction accuracy compared to true hyperspectral imaging. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. C1 [Yoon, Seung-Chul; Shin, Tae-Sung; Lawrence, Kurt C.; Heitschmidt, Gerald W.; Park, Bosoon; Gamble, Gary R.] ARS, US Natl Poultry Res Ctr, USDA, Athens, GA 30605 USA. RP Yoon, SC (reprint author), ARS, US Natl Poultry Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM seungchul.yoon@ars.usda.gov NR 19 TC 1 Z9 1 U1 2 U2 11 PU IS&T & SPIE PI BELLINGHAM PA 1000 20TH ST, BELLINGHAM, WA 98225 USA SN 1017-9909 EI 1560-229X J9 J ELECTRON IMAGING JI J. Electron. Imaging PD JUL PY 2015 VL 24 IS 4 AR 043008 DI 10.1117/1.JEI.24.4.043008 PG 11 WC Engineering, Electrical & Electronic; Optics; Imaging Science & Photographic Technology SC Engineering; Optics; Imaging Science & Photographic Technology GA CT0KW UT WOS:000362485300008 ER PT J AU Gennari, SM Ogrzewalska, MH Soares, HS Saraiva, DG Pinter, A Nieri-Bastos, FA Labruna, MB Szabo, MPJ Dubey, JP AF Gennari, Solange Maria Ogrzewalska, Maria Halina Soares, Herbert Sousa Saraiva, Danilo Goncalves Pinter, Adriano Nieri-Bastos, Fernanda Aparecida Labruna, Marcelo Bahia Juan Szabo, Matias Pablo Dubey, Jitender Prakash TI Toxoplasma gondii antibodies in wild rodents and marsupials from the Atlantic Forest, state of Sao Paulo, Brazil SO REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA LA English DT Article DE Atlantic Forest; Brazil; marsupials; rodents; Toxoplasma gondii ID NEOSPORA-CANINUM; SEROPREVALENCE; RESPONSES AB Toxoplasma gondii is a protozoan parasite that infects a large spectrum of warm-blooded animals, including humans. Small rodents and marsupials play an important role in the epidemiology of T. gondii because they are sources of infection for domestic and feral cats. Serum samples from 151 rodents and 48 marsupials, captured in the Atlantic Forest, Sao Paulo State, southeastern Brazil, were analyzed for the presence of T. gondii antibodies. Antibodies detected by the modified agglutination test (MAT >= 25) were found in 8.6% (13/151) of the rodents and 10.4% (5/48) of the marsupials, with titers ranging from 25 to 6400 and from 25 to 3200, respectively for the rodents and marsupials. Three of the eight species of rodents (Akodon spp., Oligoryzomys nigripes and Rattus norvegicus), and one from the four marsupial species (Didelphis aurita) presented positive animals. T. gondii was described for the first time in the rodent Oligoryzomys nigripes. C1 [Gennari, Solange Maria; Soares, Herbert Sousa; Saraiva, Danilo Goncalves; Nieri-Bastos, Fernanda Aparecida; Labruna, Marcelo Bahia] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, BR-05508270 Sao Paulo, SP, Brazil. [Ogrzewalska, Maria Halina] Fiocruz MS, Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Hantaviroses & Rickettsioses, BR-21045900 Rio De Janeiro, RJ, Brazil. [Pinter, Adriano] Superintendencia Controle Endemias Estado Sao Paul, Sao Paulo, SP, Brazil. [Juan Szabo, Matias Pablo] Univ Fed Uberlandia, Fac Med Vet, BR-38400 Uberlandia, MG, Brazil. [Dubey, Jitender Prakash] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. RP Gennari, SM (reprint author), Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Ave Prof Orlando Marques Paiva 87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. EM sgennari@usp.br NR 19 TC 0 Z9 0 U1 2 U2 11 PU BRAZILIAN COLL VETERINARY PARASITOLOGY PI SAO PAULO PA C/O ASSISTANT EDITOR AV PROF LINEU PRESTES, 1374, CIDADE UNIVERSITARIA, CEP 05508-900, SAO PAULO, 00000, BRAZIL SN 1984-2961 J9 REV BRAS PARASITOL V JI Rev. Bras. Parasitol. Vet. PD JUL-SEP PY 2015 VL 24 IS 3 BP 379 EP 382 DI 10.1590/S1984-29612015045 PG 4 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CS7ML UT WOS:000362267600020 PM 26444068 ER PT J AU DeKeyser, ES Dennhardt, LA Hendrickson, J AF DeKeyser, Edward S. Dennhardt, Lauren A. Hendrickson, John TI Kentucky bluegrass (Poa pratensis) Invasion in the Northern Great Plains: A Story of Rapid Dominance in an Endangered Ecosystem SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Climate change; ecosystem impacts; history of invasion; introduced species; invasion; Northern Great Plains ID SPURGE EUPHORBIA-ESULA; ATMOSPHERIC CO2; UNITED-STATES; MANAGEMENT; PRAIRIE; CONSERVATION; DAKOTA AB Kentucky bluegrass was introduced into the present-day United States in the 1600s. Since that time, Kentucky bluegrass has spread throughout the United States and Canada becoming prolific in some areas. In the past century, Kentucky bluegrass has been a presence and often a dominant species in some prairies in the Northern Great Plains. Sometime within the past few decades, Kentucky bluegrass has become the most-common species on the untilled, native prairie sites of much of North and South Dakota. In this article, we hypothesize how Kentucky bluegrass has come to dominate one of the most endangered ecosystems in North America-the prairie-through a historical, ecological, and climatological lens. We urge others to start addressing the invasion of Kentucky bluegrass with both new research and management strategies. C1 [DeKeyser, Edward S.] N Dakota State Univ, Sch Nat Resource Sci, Nat Resources Management Program, Fargo, ND 58102 USA. N Dakota State Univ, Dept Biol Sci, Fargo, ND 58102 USA. ARS, No Great Plains Res Lab, USDA, Mandan, ND 58554 USA. RP DeKeyser, ES (reprint author), N Dakota State Univ, Sch Nat Resource Sci, Nat Resources Management Program, Fargo, ND 58102 USA. EM edward.dekeyser@ndsu.edu NR 52 TC 1 Z9 1 U1 8 U2 28 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2015 VL 8 IS 3 BP 255 EP 261 DI 10.1614/IPSM-D-14-00069.1 PG 7 WC Plant Sciences SC Plant Sciences GA CS5PC UT WOS:000362129200001 ER PT J AU Jones, LJ Ostoja, SM Brooks, ML Hutten, M AF Jones, Laura J. Ostoja, Steven M. Brooks, Matthew L. Hutten, Martin TI Short-term Response of Holcus lanatus L. (Common Velvetgrass) to Chemical and Manual Control at Yosemite National Park, USA SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Hand pulling; invasive species; Sierra Nevada; meadow; Yosemite National Park ID SUCCESSIONAL GRASSLAND; SPECIES INTERACTIONS; PLANT INVASIONS; CALIFORNIA; HERBICIDE; DIVERSITY; COLONIZATION; RESTORATION; DISTURBANCE; MANAGEMENT AB One of the highest priority invasive species at both Yosemite and Sequoia and Kings Canyon national parks is Holcus lanatus L. (common velvetgrass), a perennial bunchgrass that invades mid-elevation montane meadows. Despite velvetgrass being a high priority species, there is little information available on control techniques. The goal of this project was to evaluate the short-term response of a single application of common chemical and manual velvetgrass control techniques. The study was conducted at three montane sites in Yosemite National Park. Glyphosate spot-spray treatments were applied at 0.5, 1.0, 1.5, and 2.0% concentrations, and compared with hand pulling to evaluate effects on cover of common velvetgrass, cover of other plant species, and community species richness. Posttreatment year 1 cover of common velvetgrass was 12.1% +/- 1.6 in control plots, 6.3% +/- 1.5 averaged over the four chemical treatments (all chemical treatments performed similarly), and 13.6% +/- 1.7 for handpulled plots. This represents an approximately 50% reduction in common velvetgrass cover in chemically-treated plots recoded posttreatment year 1 and no statistically significant reduction in hand pulled plots compared with controls. However, there was no treatment effect in posttreatment year 2, and all herbicide application rates performed similarly. In addition, there were no significant treatment effects on nontarget species or species richness. These results suggest that for this level of infestation and habitat type, (1) one year of hand pulling is not an effective control method and (2) glyphosate provides some level of control in the short-term without impact to nontarget plant species, but the effect is temporary as a single year of glyphosate treatment is ineffective over a two-year period. C1 [Jones, Laura J.] Natl Pk Serv, Div Resources Management & Sci, El Portal, CA 95318 USA. [Ostoja, Steven M.] US Forest Serv, Sierra Natl Forest, Clovis, CA 93611 USA. [Brooks, Matthew L.] US Geol Survey, Western Ecol Res Ctr, Yosemite Field Stn, Oakhurst, CA 93644 USA. [Hutten, Martin] US Forest Serv, Tongass Natl Forest, Wrangell, AK 99929 USA. RP Ostoja, SM (reprint author), US Forest Serv, Sierra Natl Forest, Clovis, CA 93611 USA. EM stevenmostoja@fs.fed.us FU U.S. Geological Survey Park Oriented Biological Support program; U.S. Geological Survey Invasive Species Management Program FX Susan Jones, Athena Demetry, Robin White and three anonymous reviewers provided helpful comments on earlier versions of this paper. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. This project was funded by the U.S. Geological Survey Park Oriented Biological Support program and the U.S. Geological Survey Invasive Species Management Program. NR 38 TC 0 Z9 0 U1 2 U2 13 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2015 VL 8 IS 3 BP 262 EP 268 DI 10.1614/IPSM-D-14-00060.1 PG 7 WC Plant Sciences SC Plant Sciences GA CS5PC UT WOS:000362129200002 ER PT J AU Espeland, EK Kilian, R AF Espeland, Erin K. Kilian, Robert TI Low-Dose Glyphosate Does Not Control Annual Bromes in the Northern Great Plains SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Bromus japonicas; Bromus tectorum; cheatgrass; field brome; forb diversity; herbicide; nontarget effects; perennial grasses; seed bank ID MIXED GRASS PRAIRIE; DOWNY BROME; SELECTIVE CONTROL; TECTORUM; IMPACTS; MANAGEMENT; RANGELANDS; SYSTEMS; YIELD; FIRE AB Annual bromes (downy brome and Japanese brome) have been shown to decrease perennial grass forage production and alter ecosystem functions in northern Great Plains rangelands. Large-scale chemical control might be a method for increasing rangeland forage production. Although fall application has been shown to be the most effective and least likely to impact co-occurring native species, spring germination of downy brome may reduce the efficacy of fall-only herbicide application. We assessed the impact of a low glyphosate dose rate (210 g ha (-1)) applied to rangelands in fall or in fall and spring on nontarget species and on annual brome abundance at two sites in eastern Montana over 2 yr. We tested the following hypotheses: (1) nontarget effects are greater with spring herbicide application, (2) fall and spring herbicide application are necessary for effective downy brome control, and (3) fall herbicide application is sufficient to control Japanese brome. Few nontarget effects occurred; two dicotyledonous species exhibited small increases in response to herbicide. We found that that a single fall application reduced downy brome cover and seed bank density, but after the second fall application in the following year, downy brome did not continue to show a response to herbicide. After 2 yr of fall herbicide application, Japanese brome had denser seed banks in plots where herbicide had been applied. Blanket glyphosate application on rangelands is an unreliable method for controlling annual brome invasions in the northern Great Plains. C1 [Espeland, Erin K.] ARS, USDA, Northern Plains Agr Res Lab, Pest Management Res Unit, Sidney, MT 59270 USA. [Kilian, Robert] Nat Resources Conservat Serv, USDA, Area Off, Miles City, MT 59301 USA. RP Espeland, EK (reprint author), ARS, USDA, Northern Plains Agr Res Lab, Pest Management Res Unit, Sidney, MT 59270 USA. EM erin.espeland@ars.usda.gov FU U.S. Department of Agriculture-appropriated project [5436-22000-017-00] FX Research was funded by U.S. Department of Agriculture-appropriated project 5436-22000-017-00. Thanks to Maureen O'Mara for patient and painstaking field and lab assistance. Thanks also to Vicki Eskridge, Carol Karres, Cheryl Olson, Darcy Hammond, and Christie Youngs. Tom Nelson and George and Linda Carson generously provided study sites. John Gaskin, Dustin Strong, Jane Mangold, and two anonymous reviewers made suggestions that improved the manuscript. Thanks to Dan Bergstrom at Wilbur Ellis for conducting the spraying and supplying the chemical. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 42 TC 0 Z9 0 U1 3 U2 10 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2015 VL 8 IS 3 BP 334 EP 340 DI 10.1614/IPSM-D-15-00004.1 PG 7 WC Plant Sciences SC Plant Sciences GA CS5PC UT WOS:000362129200010 ER PT J AU Laflamme, G Broders, K Cote, C Munck, I Iriarte, G Innes, L AF Laflamme, Gaston Broders, Kirk Cote, Chantal Munck, Isabel Iriarte, Gloria Innes, Louise TI Priority of Lophophacidium over Canavirgella: taxonomic status of Lophophacidium dooksii and Canavirgella banfieldii, causal agents of a white pine needle disease SO MYCOLOGIA LA English DT Article DE Phacidiaceae; Pinus strobus; Rhytismataceae ID FUNGUS; DAMAGE AB In 2009 unusual white pine needle discoloration was observed in eastern Canada and northeastern USA. While the symptoms were similar in most pine stands, the disease was diagnosed as Canavirgella banfieldii in several locations and Dooks needle blight caused by Lophophacidium dooksii in others. Because of the similarities in symptom development and morphological characters of the causal agents, it was suspected that C. banfieldii and L. dooksii are either the same or closely related species. To test this hypothesis we examined several collections representing C. banfieldii and L. dooksii, including the two type specimens. Phylogenedc analyses of nuc internal transcribed spacer rDNA sequences confirm the synonymy of C. banfieldii with the earlier described L. dooksii and provide the first evidence of the close evolutionary relationship of L. dooksii to other pine pathogens. C1 [Laflamme, Gaston; Cote, Chantal] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Quebec City, PQ G1V 4C7, Canada. [Broders, Kirk; Iriarte, Gloria] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Munck, Isabel] USDA Forest Serv, State & Private Forestry, Durham, NH 03824 USA. [Innes, Louise] Minist Ressources Nat Quebec, Direct Protect Forets, Quebec City, PQ G1P 3W8, Canada. RP Laflamme, G (reprint author), Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, 1055 PEPS, Quebec City, PQ G1V 4C7, Canada. EM laflamme@nrcan-rncan.gc.ca OI Munck, Isabel/0000-0001-6889-9196 NR 28 TC 3 Z9 3 U1 1 U2 5 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2015 VL 107 IS 4 BP 745 EP 753 DI 10.3852/14-096 PG 9 WC Mycology SC Mycology GA CS2RL UT WOS:000361918700008 PM 25977210 ER PT J AU Tolk, JA Evett, SR Schwartz, RC AF Tolk, Judy A. Evett, Steven R. Schwartz, Robert C. TI Field-Measured, Hourly Soil Water Evaporation Stages in Relation to Reference Evapotranspiration Rate and Soil to Air Temperature Ratio SO VADOSE ZONE JOURNAL LA English DT Article ID BARE SOIL; MODEL; CROP; GROWTH AB Upon wetting, soil water evaporation (E) is controlled by available energy (Stage 1, or S1), and once water becomes limiting, also by soil hydraulic characteristics (Stage 2, or S2). Determination of the transitioning between stages is important for estimating E. The objectives of this research were to compare changes in E in relation to reference evapotranspiration (ETo), or E ETo(-1), and to the ratio of surface soil temperature (T-s) and air temperature (T-a), or T-s T-a(-1), using its change from < 1 to > 1 as a means to identify stage transitions. Hourly E measurements were made in 17 measurement runs after irrigations ranging from 9 to 52 mm in weighing lysimeters containing clay loam, silt loam, sandy loam, and fine sand at Bushland, TX. On the day of irrigation, E exceeded ETo by 21% on average. The 3-d total E losses from bare soils averaged 47% of irrigation amounts > 30 mm, 72% of irrigation amounts < 30 mm, and nighttime E averaged 28% of total E losses. On the day of irrigation, E/ETo relationships were similar for the four soils, indicating that soil texture had no effect on E/ETo during S1. The T-s T-a(-1) ratio exhibited a consistent response to drying rate changes. For E > ETo, T-s Ta-1 remained <= 0.9, but would be >= 1.0 when E was <= ETo. By using criteria based on T-s T-a(-1), estimates of readily available (REW) and total evaporable water (TEW) were variable but often larger for REW and within 3.5 mm for TEW, when compared with published values. C1 [Tolk, Judy A.; Evett, Steven R.; Schwartz, Robert C.] USDA ARS, Bushland, TX 79012 USA. RP Tolk, JA (reprint author), USDA ARS, PO Drawer 10, Bushland, TX 79012 USA. EM judy.tolk@ars.usda.gov NR 38 TC 2 Z9 2 U1 4 U2 11 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD JUL PY 2015 VL 14 IS 7 DI 10.2136/vzj2014.07.0079 PG 14 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CS4SC UT WOS:000362065200003 ER PT J AU Tuo, WB Zarlenga, D Hebert, D Miramontes, E Fetterer, R AF Tuo, Wenbin Zarlenga, Dante Hebert, Deborah Miramontes, Eliseo Fetterer, Raymond TI A Two-Step Method to Generate Highly-Purified Nematode Eggs from Feces: Sucrose Flotation Followed by Density Gradient Centrifugation Using Lymphocyte Separation Medium SO COMPARATIVE PARASITOLOGY LA English DT Article DE Ostertagia ostertagi; gastrointestinal nematode; parasite; egg; isolation AB Many modern-day studies require parasite samples essentially free of environmental contamination. Techniques used to prepare gastrointestinal nematode eggs from livestock are sufficient for biological studies but fall woefully short of generating pure preparations for downstream molecular, biochemical, and immunological studies. Consequently, a method to produce highly purified nematode parasite eggs free from fecal contamination is needed. The present study compared different procedures for egg isolation and attempted to improve the purity of Ostertagia ostertagi eggs isolated from cattle feces. The Wisconsin method using saturated sucrose has been used widely to enrich eggs from feces and is adequate for identification and counting. A more-recently developed method using the combination of salt and sucrose is robust and consistent and can be used to isolate eggs with much higher purity; however, residual fecal materials are still present in the egg preparations, which could account for large levels of nonspecific DNA and RNA contamination in the final samples. While large numbers of eggs can be harvested from the medium of overnight, in vitro-cultured adult worms, this method is labor intensive, involves euthanasia of animals, and requires the purification of adult worms to high purity and subsequent culturing to acquire the eggs. In addition, a large proportion of the eggs initiate development and hatch during isolation. In the current method, Ostertagia eggs free from fecal contamination were secondarily purified using lymphocyte separation medium (LSM) from eggs previously enriched by the Wisconsin method. The egg recovery following the LSM step was 100%. The results indicate that this two-step method involving sucrose and LSM was simple, rapid, nonselective, and greatly improved the purity of Ostertagia eggs. This method will have broad application for isolating eggs of the superfamily of Trichostrongyloidea, which includes the most-important nematode parasites infecting livestock. C1 [Tuo, Wenbin; Zarlenga, Dante; Hebert, Deborah; Miramontes, Eliseo; Fetterer, Raymond] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Tuo, WB (reprint author), ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM wenbin.tuo@ars.usda.gov; dante.zarlenga@ars.usda.gov; deborah.hebert@ars.usda.gov; eliseo.miramontes@ars.usda.gov; raymond.fetterer@ars.usda.gov NR 6 TC 1 Z9 1 U1 1 U2 6 PU HELMINTHOLOGICAL SOC WASHINGTON PI LAWRENCE PA C/O ALLEN PRESS INC, 1041 NEW HAMPSHIRE ST, ACCT# 141866, LAWRENCE, KS 66044 USA SN 1525-2647 EI 1938-2952 J9 COMP PARASITOL JI Comp. Parasitol. PD JUL PY 2015 VL 82 IS 2 BP 275 EP 279 PG 5 WC Parasitology; Zoology SC Parasitology; Zoology GA CR7VT UT WOS:000361560600016 ER PT J AU Maggioli, MF Palmer, MV Vordermeier, HM Whelan, AO Fosse, JM Nonnecke, BJ Waters, WR AF Maggioli, Mayara F. Palmer, Mitchell V. Vordermeier, H. Martin Whelan, Adam O. Fosse, James M. Nonnecke, Brian J. Waters, W. Ray TI Application of Long-term cultured Interferon-gamma Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Immunology; Issue 101; Immunology; bovine tuberculosis; CD4 T cells; vaccine ID BOVINE TUBERCULOSIS; PROTECTION; VACCINATION; INFECTION; VACCINES; VIRUS; PROFILES; BET; PERSISTENCE; COMMITMENT AB Effector and memory T cells are generated through developmental programing of naive cells following antigen recognition. If the infection is controlled up to 95 % of the T cells generated during the expansion phase are eliminated (i.e., contraction phase) and memory T cells remain, sometimes for a lifetime. In humans, two functionally distinct subsets of memory T cells have been described based on the expression of lymph node homing receptors. Central memory T cells express C-C chemokine receptor 7 and CD45RO and are mainly located in T-cell areas of secondary lymphoid organs. Effector memory T cells express CD45RO, lack CCR7 and display receptors associated with lymphocyte homing to peripheral or inflamed tissues. Effector T cells do not express either CCR7 or CD45RO but upon encounter with antigen produce effector cytokines, such as interferon-gamma. Interferon-gamma release assays are used for the diagnosis of bovine and human tuberculosis and detect primarily effector and effector memory T cell responses. Central memory T cell responses by CD4(+)T cells to vaccination, on the other hand, may be used to predict vaccine efficacy, as demonstrated with simian immunodeficiency virus infection of non-human primates, tuberculosis in mice, and malaria in humans. Several studies with mice and humans as well as unpublished data on cattle, have demonstrated that interferon-gamma ELISPOT assays measure central memory T cell responses. With this assay, peripheral blood mononuclear cells are cultured in decreasing concentration of antigen for 10 to 14 days (long-term culture), allowing effector responses to peak and wane; facilitating central memory T cells to differentiate and expand within the culture. C1 [Maggioli, Mayara F.; Palmer, Mitchell V.; Nonnecke, Brian J.; Waters, W. Ray] USDA ARS, Natl Anim Dis Ctr, Infect Bacterial Dis Res Unit, Washington, DC 20250 USA. [Maggioli, Mayara F.] Iowa State Univ, Coll Vet Med, Dept Vet Pathol, Iowa City, IA USA. [Vordermeier, H. Martin; Whelan, Adam O.] UK Vet Labs Agcy, Washington, DC USA. [Fosse, James M.] USDA, Anim & Plant Hlth Inspect Serv, Natl Ctr Anim Hlth, Visual Serv, Washington, DC USA. RP Maggioli, MF (reprint author), USDA ARS, Natl Anim Dis Ctr, Infect Bacterial Dis Res Unit, Washington, DC 20250 USA. EM mayara.maggioli@ars.usda.gov RI APHA, Staff publications/E-6082-2010; Vordermeier, H Martin/C-6936-2011 FU USDA; ARS Cris [3625-32000-104]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67015-30736] FX Research was supported by USDA, ARS Cris: 3625-32000-104 and Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-30736 from the USDA National Institute of Food and Agriculture. We thank Jessica Pollock, Emma Frimml-Morgan, Shelly Zimmerman, Kristin Bass, Bruce Pesch, Molly Stafne, Allen Jensen, and Tracy Porter for their excellent technical assistance as well as Rebecca Madison, Doug Ewing, Katie Pille, Jay Steffen, David Lubbers, Robin Zeisness, and David Panthen for the excellent care and handling of animals. NR 39 TC 1 Z9 1 U1 1 U2 4 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD JUL PY 2015 IS 101 AR e52833 DI 10.3791/52833 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR7ND UT WOS:000361536200029 PM 26275095 ER PT J AU Webb, KM Wintermantel, WM Kaur, N Prenni, JE Broccardo, CJ Wolfe, LM Hladky, LL AF Webb, Kimberly M. Wintermantel, William M. Kaur, Navneet Prenni, Jessica E. Broccardo, Carolyn J. Wolfe, Lisa M. Hladky, Laura L. TI Differential abundance of proteins in response to Beet necrotic yellow vein virus during compatible and incompatible interactions in sugar beet containing Rz1 or Rz2 SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Sugarbeet; Beta vulgaris; Rhizomania; BNYVV; Host resistance; Plant proteomics; Virus-host interactions ID TANDEM MASS-SPECTROMETRY; P25 PATHOGENICITY FACTOR; RHIZOMANIA RESISTANCE; UNITED-STATES; SUPEROXIDE DISMUTASES; STATISTICAL-MODEL; HOST-RESISTANCE; ACTIVE OXYGEN; PCR PRODUCTS; SEED VIGOR AB Rhizomania, caused by Beet necrotic yellow vein virus (BNYVV), is an important disease affecting sugar beet. Control is achieved through planting of resistant varieties; however, following the introduction of Rz1, new pathotypes that overcome resistance have appeared. To understand how BNYVV overcomes resistance, we examined quantitative protein differences during compatible and incompatible interactions when sugar beet is infected with either a traditional A-type strain or with an Rz1 resistance breaking strain. Proteomic data suggest distinct biochemical pathways are induced during compatible and incompatible sugar beet interactions with BNYVV. Pathways including the detoxification of reactive oxygen species, UB/proteasome, and photosynthesis should be studied in more depth to characterize roles in symptom development. Published by Elsevier Ltd. C1 [Webb, Kimberly M.] USDA ARS, Sugar Beet Res Unit, Ft Collins, CO 80526 USA. [Wintermantel, William M.; Kaur, Navneet; Hladky, Laura L.] USDA ARS, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. [Prenni, Jessica E.; Broccardo, Carolyn J.; Wolfe, Lisa M.] Colorado State Univ, Prote & Metabol Facil, Microbiol C130, Ft Collins, CO 80523 USA. RP Webb, KM (reprint author), USDA ARS, Sugar Beet Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM Kimberly.webb@ars.usda.gov OI Prenni, Jessica/0000-0002-0337-8450 FU Beet Sugar Development Foundation; California Beet Growers Association; Sugar Beet Research and Education Board of Minnesota and North Dakota; Western Sugar Cooperative Joint Research Committee FX This work was supported by the Beet Sugar Development Foundation, California Beet Growers Association, the Sugar Beet Research and Education Board of Minnesota and North Dakota, and the Western Sugar Cooperative Joint Research Committee. We thank KWS for providing seed for the near-isogenic sugar beet lines used in this study. We Paul A. Covey and Arturo A. Cortez for maintaining plants and assistance in processing samples for testing, and Addison Reed for assistance in protein annotation and identification of putative function. We also thank the PRIDE team for assistance with deposit of the mass spectrometry data into the ProteomeXchange database. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 77 TC 2 Z9 2 U1 5 U2 16 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD JUL PY 2015 VL 91 BP 96 EP 105 DI 10.1016/j.pmpp.2015.06.006 PG 10 WC Plant Sciences SC Plant Sciences GA CS1WZ UT WOS:000361860900013 ER PT J AU Armstrong, PR Maghirang, EB Pearson, TC AF Armstrong, Paul R. Maghirang, Elizabeth B. Pearson, Tom C. TI Detecting and Segregating Black Tip-Damaged Wheat Kernels Using Visible and Near-Infrared Spectroscopy SO CEREAL CHEMISTRY LA English DT Article AB Detection of individual wheat kernels with black tip symptom (BTS) and black tip damage (BTD) was demonstrated with near-infrared reflectance spectroscopy (NIRS) and silicon light-emitting-diode (LED) based instruments. The two instruments tested, a single-kernel NIRS instrument (SKNIRS) and a silicon LED-based single-kernel high-speed sorter (SiLED-SKS) were both developed by the Stored Product Insect and Engineering Research Unit, Center for Grain and Animal Health Research, USDA Agricultural Research Service. BTD was classified into four levels for the study ranging from sound, symptomatic (BTS) at two levels, and damaged (BTD). Discriminant analysis models for the SKNIRS instrument could distinguish sound undamaged kernels well, correctly classifying kernels 80% of the time. Damaged kernels were classified with 67% accuracy and symptomatic kernels at about 44%. Higher classification accuracy (81-87%) was obtained by creating only two groupings: 1) combined sound and lightly symptomatic kernels and 2) combined heavily symptomatic and damaged kernels. A linear regression model was developed from the SiLED-SKS sorted fractions to predict the percentage of combined BTS and BTD kernels in a sample. The model had an R-2 of 0.64 and a standard error of prediction of 7.4%, showing it had some measurement ability for BTS and BTD. The SiLED-SKS correctly classified and sorted out 90% of BTD and 66% of BTS for all 28 samples after three passes through the sorter. These instruments can serve as important tools for plant breeders and grading facilities of the wheat industry that require timely and objective determination and sorting of different levels of black tip present in wheat samples. C1 [Armstrong, Paul R.; Maghirang, Elizabeth B.] ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect & Engn Res Unit, Manhattan, KS 66502 USA. [Pearson, Tom C.] Amgen Inc, Thousand Oaks, CA 91320 USA. RP Armstrong, PR (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect & Engn Res Unit, Manhattan, KS 66502 USA. EM paul.armstrong@ars.usda.gov NR 16 TC 0 Z9 0 U1 3 U2 4 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD JUL-AUG PY 2015 VL 92 IS 4 BP 358 EP 363 DI 10.1094/CCHEM-09-14-0201-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CQ7XG UT WOS:000360818900003 ER PT J AU Chen, MH McClung, AM AF Chen, Ming-Hsuan McClung, Anna M. TI Effects of Cultivars, Organic Cropping Management, and Environment on Antioxidants in Whole Grain Rice SO CEREAL CHEMISTRY LA English DT Article ID NUTRITIONAL QUALITY; GAMMA-ORYZANOL; CANCER CELLS; BROWN RICE; SATIVA L.; VITAMIN-E; METABOLISM; PERICARP; PHENOLS; FOODS AB Whole grain rice contains functional antioxidants such as phenolics, flavonoids (including proanthocyanidins), vitamin E homologs (tocopherols and tocotrienols), and gamma-oryzanol that have positive effects on human health. These antioxidants are secondary metabolites in plants that can be induced under external stress. The objectives of this study were to quantify the effects of cultivars, crop management method (organic and conventional), and growing environment on the concentrations of these antioxidants in whole grain rice. Cultivars and environment contributed to a higher percentage of variation in the concentrations of these antioxidants than did crop management method. Cultivars accounted for a greater proportion of the variation than environment for all traits except total tocotrienols and gamma-oryzanol. Cultivars that are high in concentrations of these antioxidants were identified, but no one cultivar contained the highest concentration of all antioxidants evaluated. These cultivar differences indicate that improvement for phytochemical and antioxidant traits can be accomplished through traditional breeding. Because of the limited effect of crop management on these antioxidants, choice of cultivar should be the focus for organic production of whole grain rice high in these antioxidants. C1 [Chen, Ming-Hsuan; McClung, Anna M.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Chen, MH (reprint author), ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM ming.chen@ars.usda.gov NR 31 TC 1 Z9 1 U1 4 U2 9 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD JUL-AUG PY 2015 VL 92 IS 4 BP 364 EP 369 DI 10.1094/CCHEM-11-14-0240-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CQ7XG UT WOS:000360818900004 ER PT J AU Meepagala, KM Johnson, RD Techen, N Wedge, DE Duke, SO AF Meepagala, Kumudini M. Johnson, Robert D. Techen, Natascha Wedge, David E. Duke, Stephen O. TI Phomalactone from a Phytopathogenic Fungus Infecting ZINNIA elegans (ASTERACEAE) Leaves SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Phomalactone; Catenioblin A; Phytotoxin; Asteraceae; Nigrospora sphaerica; Zinnia elegans ID NATURAL-PRODUCTS; ENDOPHYTIC FUNGI; PLANT-PATHOGENS; PHYTOTOXINS; METABOLITES; HERBICIDES; DISCOVERY; GROWTH; MODES AB Zinnia elegans Jacq. plants are infected by a fungus that causes dark red spots with necrosis on leaves, particularly in late spring to the middle of summer in the Mid-South of the United States. This fungal disease causes the leaves to wilt and eventually kills the plant. The fungus was isolated, cultured in potato dextrose broth, and identified as Nigrospora sphaerica by molecular techniques. Two major lactone metabolites (phomalactone and catenioblin A) were isolated from liquid culture of N. sphaerica isolated from Z. elegans. When injected into leaves of Z. elegans, phomalactone caused lesions similar to those of the fungus. The lesion sizes were proportional to the concentration of the phomalactone. Phomalactone, but not catenioblin A, was phytotoxic to Z. elegans and other plant species by inhibition of seedling growth and by causing electrolyte leakage from photosynthetic tissues of both Z. elegans leaves and cucumber cotyledons. This latter effect may be related to the wilting caused by the fungus in mature Z. elegans plants. Phomalactone was moderately fungicidal to Coletotrichum fragariae and two Phomopsis species, indicating that the compound may keep certain other fungi from encroaching into plant tissue that N. sphaerica has infected. Production of large amounts of phomalactone by N. sphaerica contributes to the pathogenic behavior of this fungus, and may have other ecological functions in the interaction of N. sphaerica with other fungi. This is the first report of isolation of catenioblin A from a plant pathogenic fungus. The function of catenioblin A is unclear, as it was neither significantly phyto-nor fungitoxic. C1 [Meepagala, Kumudini M.; Johnson, Robert D.; Wedge, David E.; Duke, Stephen O.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Techen, Natascha] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod, University, MS 38677 USA. RP Meepagala, KM (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, POB 1848, University, MS 38677 USA. EM kmeepaga@olemiss.edu NR 38 TC 0 Z9 0 U1 1 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2015 VL 41 IS 7 BP 602 EP 612 DI 10.1007/s10886-015-0602-x PG 11 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CR6OC UT WOS:000361465800002 PM 26133676 ER PT J AU Zou, YF Millar, JG Blackwood, JS Van Duzor, R Hanks, LM Mongold-Diers, JA Wong, JCH Ray, AM AF Zou, Yunfan Millar, Jocelyn G. Blackwood, J. Scott Van Duzor, Ryan Hanks, Lawrence M. Mongold-Diers, Judith A. Wong, Joseph C. H. Ray, Ann M. TI (2S,4E)-2-Hydroxy-4-octen-3-one, a Male-Produced Attractant Pheromone of the Cerambycid Beetle Tylonotus bimaculatus SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Semiochemical; Longhorned beetle; Aggregation pheromone; 3-hydroxy-4-octen-2-one; Cerambycinae ID COLEOPTERA CERAMBYCIDAE; AGGREGATION PHEROMONES; SEX-PHEROMONE; COMPONENTS; VOLATILES; PHENOLOGY; FUSCUMOL; LURES AB We report the identification of a novel pheromone structure from males of the cerambycid beetle Tylonotus bimaculatus Haldeman (Cerambycinae: Hesperophanini), a species native to eastern North America. Volatiles collected from adult males contained (2S,4E)-2-hydroxyoct-4-en-3-one (71 %), (3R,4E)-3-hydroxyoct-4-en-2-one (15 %), (E)-4octen- 2,3-dione (13 %), and 2,3-octanedione (1.5 %). Four independent field bioassays with synthetic compounds confirmed that adults of both sexes were attracted by the racemate of the major component, (E)-2-hydroxyoct-4-en-3-one. No other cerambycid species were attracted in significant numbers. Attraction of both sexes is consistent with the male-produced pheromones of many other species in the subfamily Cerambycinae, but T. bimaculatus is unusual in having a pheromone chemistry that is so far unique among species in that subfamily. C1 [Zou, Yunfan; Millar, Jocelyn G.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Millar, Jocelyn G.] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA. [Blackwood, J. Scott; Van Duzor, Ryan] USDA APHIS PPQ, Des Plaines, IL 60018 USA. [Hanks, Lawrence M.; Mongold-Diers, Judith A.; Wong, Joseph C. H.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Ray, Ann M.] Xavier Univ, Dept Biol, Cincinnati, OH 45207 USA. RP Millar, JG (reprint author), Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. EM jocelyn.millar@ucr.edu FU Alphawood Foundation of Chicago; USDA APHIS PPQ [14-8130-0362-CA] FX We thank Steve Buck and the University of Illinois Committee on Natural Areas, the Vermilion County Conservation District, and the Forest Preserve Districts of Cook and DuPage Counties for access to field sites. We gratefully acknowledge financial support from The Alphawood Foundation of Chicago and USDA APHIS PPQ (agreement 14-8130-0362-CA). NR 33 TC 1 Z9 1 U1 2 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2015 VL 41 IS 7 BP 670 EP 677 DI 10.1007/s10886-015-0603-9 PG 8 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CR6OC UT WOS:000361465800009 PM 26149425 ER PT J AU Martin, DT Baribursa, D Huesing, JE Williams, SB Murdock, LL AF Martin, D. T. Baribursa, D. Huesing, J. E. Williams, S. B. Murdock, L. L. TI PICS bags protect wheat grain, Triticum aestivum (L.), against rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae) SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Hermetic; Triple bag; Postharvest; Storage; Wheat; Sitophilus oryzae ID COWPEA GRAIN; POSTHARVEST PRESERVATION; STORAGE; NIGER; DIFFUSION; BRUCHIDS; DENSITY; MAIZE; O-2; CO2 AB Purdue Improved Crop Storage Bags (PICS) bags are hermetic containers consisting of two inner layers of high-density polyethylene (HDPE) surrounded by an outer woven polypropylene bag. They have proven effective in preventing losses of stored cowpea grain to the cowpea bruchid, Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae) and perform well with other dry grains attacked by several species of postharvest pests. We tested PICS bags against the rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae), a common stored grain pest. Storing rice weevil infested wheat in PICS bags arrested weevil population growth and preserved the grain well. The air surrounding infested wheat held in PICS bags had lower oxygen and higher carbon dioxide levels compared to the ambient atmosphere. Seed germination declined over time in infested PICS bags, but less so than did grain held in infested woven bags. Low-cost, durable, pesticide-free PICS technology offers an inexpensive, practical and useful way to protect wheat against rice weevil. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Martin, D. T.; Baribursa, D.; Williams, S. B.; Murdock, L. L.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Huesing, J. E.] USDA, Washington, DC 20250 USA. RP Murdock, LL (reprint author), Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. EM murdockl@purdue.edu NR 22 TC 2 Z9 2 U1 2 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD JUL PY 2015 VL 63 BP 22 EP 30 DI 10.1016/j.jspr.2015.05.001 PG 9 WC Entomology SC Entomology GA CR5WD UT WOS:000361414200004 ER PT J AU Flinn, PW Arthur, FH Throne, JE Friesen, KS Hartzer, KL AF Flinn, P. W. Arthur, F. H. Throne, J. E. Friesen, K. S. Hartzer, K. L. TI Cold temperature disinfestation of bagged flour SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Tribolium castaneum; Freezing mortality; Stored-product insect control ID STORED-PRODUCT; METHYL-BROMIDE; ALTERNATIVES; AERATION; INSECTS; WHEAT; TIME; EGGS AB We conducted studies using a commercial freezer maintained at -17.8 degrees C to determine the time needed to kill Tribolium castaneum eggs in a pallet of flour. Each bag weighed 22.7 kg, and there were 5 bags in each of 10 layers. The dimensions of the pallet were 109-cm wide by 132-cm long by 123-cm tall, and the weight of the stacked pallet was approximately 1152 kg. We conducted tests for nine internal goal temperatures of -12, -10, -8, -6, -4, -2, 0, 4 and 8 degrees C. Internal temperatures in the most central location of the flour pallet reached: -11.0, -9.4, -6.9, -5.0, -3.5, -1.6, -0.1, 3.3, and 5.6 degrees C and were achieved after 11.0, 9.1, 8.9, 7.2, 6.7, 5.8, 5.5, 5.2, and 4.2 days, respectively. For treatments where the goal temperature for the center bag ranged from -12 to 4 degrees C, egg mortality was 100% in bags located in both the periphery and in the center of the pallet. When the temperature goal for the center bag was 8 degrees C, 7 +/- 2.5% of the eggs survived in bags located near the center of the pallet. Our data showed that temperatures that follow the dynamic temperature curve that takes place over 24.2 days (cool down and warm up for the 0 degrees C temperature goal) resulted in 100% mortality of T. castaneum eggs. The reason for the difference in mortality for a static compared to a dynamic temperature treatment may be due to the fact that the dynamic temperature treatment occurs over a much longer duration. The fact that the treatment only required 5.5 days in the freezer before it could be shipped makes it a practical method to disinfest pallets of flour, especially because the bags do not need to be removed from the pallet and no chemicals are used. Published by Elsevier Ltd. C1 [Flinn, P. W.; Arthur, F. H.; Throne, J. E.; Friesen, K. S.; Hartzer, K. L.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Flinn, PW (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM pwflinn@gmail.com NR 21 TC 2 Z9 2 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD JUL PY 2015 VL 63 BP 42 EP 46 DI 10.1016/j.jspr.2015.06.001 PG 5 WC Entomology SC Entomology GA CR5WD UT WOS:000361414200007 ER PT J AU Hou, LX Hou, JC Li, ZX Johnson, JA Wang, SJ AF Hou, Lixia Hou, Juncai Li, Zhongxin Johnson, Judy A. Wang, Shaojin TI Validation of radio frequency treatments as alternative non-chemical methods for disinfesting chestnuts SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Chestnut; Disinfestation; Quality; Radio frequency; Validation ID THERMAL-DEATH KINETICS; TRANSITELLA WALKER LEPIDOPTERA; CONTROL CODLING MOTH; IN-SHELL WALNUTS; INSECT CONTROL; POSTHARVEST TREATMENT; MACADAMIA NUTS; QUALITY; ENERGY; PYRALIDAE AB Chemical fumigation has been widely used to control insects in postharvest chestnuts but is inherent dangers when using fumigants. The purpose of this study was to validate application of radio frequency (RF) treatments for disinfesting chestnuts as an alternative to chemical fumigation. A practical process protocol was developed to control insect pests in chestnuts using a 27.12 MHz free-running oscillator RF system. Fifth-instar yellow peach moth, Conogethes punctiferalis, more heat tolerant than chestnut weevil, Curculio elephas, under three temperature and time combinations using a heating block system, was selected as the targeted insect to validate the RF treatment protocol. Mortality of fifth-instar C punctiferalis increased with increasing holding time at 55 degrees C using RF heating and reached 100% while holding in hot air for at least 5 min. Furthermore, there was no significant quality difference in color, fat, firmness, moisture content, protein, and soluble sugar content of chestnuts observed between RF treatments and controls. RF treatment methods hold potential to scale up for industrial applications of disinfesting chestnuts. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Hou, Lixia; Hou, Juncai; Wang, Shaojin] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Li, Zhongxin] Xinjiang Acad Agr Sci, Agr Mechanizat Inst, Urumqi, Xinjiang, Peoples R China. [Johnson, Judy A.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Wang, Shaojin] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Wang, SJ (reprint author), Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM shaojinwang@nwsuaf.edu.cn FU Open Fund for Key Laboratory of Physical Processing of Agricultural Product in Jiangsu Province [JAPP2013-2]; General Program of National Natural Science Foundation of China [31371853]; Shaanxi Agricultural Science and Technology Innovation and Research Project [2013K01-50] FX This research was conducted in the College of Mechanical and Electronic Engineering, Northwest A&F University, and supported by research grants from Open Fund for Key Laboratory of Physical Processing of Agricultural Product in Jiangsu Province (JAPP2013-2), General Program of National Natural Science Foundation of China (31371853) and Shaanxi Agricultural Science and Technology Innovation and Research Project (2013K01-50). The authors thank Xiuyun Zhang for helps on chestnut quality evaluations. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 41 TC 7 Z9 7 U1 4 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD JUL PY 2015 VL 63 BP 75 EP 79 DI 10.1016/j.jspr.2015.07.004 PG 5 WC Entomology SC Entomology GA CR5WD UT WOS:000361414200014 ER PT J AU Everman, JL Eckstein, TM Roussey, J Coussens, P Bannantine, JP Bermudez, LE AF Everman, Jamie L. Eckstein, Torsten M. Roussey, Jonathan Coussens, Paul Bannantine, John P. Bermudez, Luiz E. TI Characterization of the inflammatory phenotype of Mycobacterium avium subspecies paratuberculosis using a novel cell culture passage model SO MICROBIOLOGY-SGM LA English DT Article ID NATURALLY INFECTED CATTLE; BACILLUS-CALMETTE-GUERIN; BOVINE EPITHELIAL-CELLS; JOHNES-DISEASE; GENE-EXPRESSION; INTRACELLULAR PHENOTYPE; VACCINE CANDIDATES; IN-VITRO; MACROPHAGES; VIRULENCE AB Understanding the pathogenic mechanisms of Mycobacterium avium subspecies paratuberculosis (MAP) and the host responses to Johne's disease is complicated by the multi-faceted disease progression, late-onset host reaction and the lack of available ex vivo infection models. We describe a novel cell culture passage model that mimics the course of infection in vivo. The developed model simulates the interaction of MAP with the intestinal epithelial cells, followed by infection of macrophages and return to the intestinal epithelium. MAP internalization triggers a minimal inflammatory response. After passage through a macrophage phase, bacterial reinfection of MDBK epithelial cells, representing the late phase of intestinal mucosal infection, is associated with increased synthesis of the pro-inflammatory transcripts of IL-6, CCL5, IL-8 and IL-18, paired with decreased levels of TGF beta. Transcriptome analysis of MAP from each stage of epithelial cell infection identified increased expression of lipid biosynthesis and lipopeptide modification genes in the inflammatory phenotype of MAP. Total lipid analysis by HPLC-ES/MS indicates different lipidomic profiles between the two phenotypes and a unique set of lipids composing the inflammatory MAP phenotype. The presence of selected upregulated lipid-modification gene transcripts in samples of ileal tissue from cows diagnosed with Johne's disease supports and validates the model. By using the relatively simple cell culture passage model, we show that MAP alters its lipid composition during intracellular infection and acquires a pro-inflammatory phenotype, which likely is associated with the inflammatory phase of Johne's disease. C1 [Everman, Jamie L.; Bermudez, Luiz E.] Oregon State Univ, Dept Microbiol, Coll Sci, Corvallis, OR 97331 USA. [Everman, Jamie L.; Bermudez, Luiz E.] Oregon State Univ, Coll Vet Med, Dept Biomed Sci, Corvallis, OR 97331 USA. [Eckstein, Torsten M.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Roussey, Jonathan; Coussens, Paul] Michigan State Univ, Comparat Med & Integrat Biol Program, E Lansing, MI 48824 USA. [Coussens, Paul] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. [Bannantine, John P.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Bermudez, LE (reprint author), Oregon State Univ, Dept Microbiol, Coll Sci, Corvallis, OR 97331 USA. EM Luiz.bermudez@oregonstate.edu OI Bannantine, John/0000-0002-5692-7898 FU Foundation for Microbiology in San Francisco; Agriculture Experimental Service, Oregon State University FX We would like to thank Caprice Rosato for technical assistance with the Axon4000 Slide Reader and hybridization analysis, and Sadie Rice for technical assistance with CCL5 ELISAs. This work was funded by the Foundation for Microbiology in San Francisco, and by the Agriculture Experimental Service, Oregon State University NR 62 TC 1 Z9 1 U1 0 U2 2 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1350-0872 EI 1465-2080 J9 MICROBIOL-SGM JI Microbiology-(UK) PD JUL PY 2015 VL 161 BP 1420 EP 1434 DI 10.1099/mic.0.000106 PN 7 PG 15 WC Microbiology SC Microbiology GA CR5VG UT WOS:000361411900012 PM 25957310 ER PT J AU Michaud, J Cordell, S Cole, TC Ostertag, R AF Michaud, Jene Cordell, Susan Cole, T. Colleen Ostertag, Rebecca TI Drought in an Invaded Hawaiian Lowland Wet Forest SO PACIFIC SCIENCE LA English DT Article ID CLIMATE-CHANGE; WATER-USE; MORTALITY; RAINFALL; TRAITS; PLANT; ISLANDS; TREES; PRECIPITATION; VULNERABILITY AB In this study we examined historic drought frequency and hydrologic effects of removing invasive plants from one of the few remaining Hawaiian wet lowland forests, near Hilo, Hawai'i. We developed a conceptual and statistical model of Hilo droughts using historic rainfall and pan evaporation data and discovered that episodes of low soil moisture were most likely from January to March but also occurred in June or July. Field measurements were taken in four pairs of plots. Nonnative woody and herbaceous species were removed from four plots; control plots were undisturbed. Soil water potential measurements documented partial soil drying in control plots, but not removal plots, during droughts with recurrence intervals of 2-3 yr. Drier soils exhibited strong small-scale heterogeneity in soil water potential that presumably reflects macroporosity in the young 'a'a lava flow substrate. Transpiration from and rainfall interception by the dense canopy of nonnative species were most likely responsible for drier conditions in control plots. Removal plots experienced changes to shading, midday vapor pressure deficit, albedo, and aerodynamic resistance, but it appears that hydrologic impact of these variables was minor. We suggest that efforts to restore Hawaiian tropical rain forests should consider drought resilience as one objective, among many, of a restoration program. Germinating seeds, shallow-rooted saplings and deeper-rooted mature trees may respond differently to hydrologic effects of removing invasive plants. C1 [Michaud, Jene] Univ Hawaii, Dept Geol, Hilo, HI 96720 USA. [Cordell, Susan; Cole, T. Colleen] US Dept Agr Forest Serv, Inst Pacific Isl Forestry, Hilo, HI 96720 USA. [Ostertag, Rebecca] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. RP Michaud, J (reprint author), Univ Hawaii, Dept Geol, 200 West Kawili St, Hilo, HI 96720 USA. EM jene@hawaii.edu FU U.S. Department of Agriculture Forest Service Institute of Pacific Islands Forestry; NSF EPSCoR [0237065, 0554657] FX This research was funded and facilitated through the U.S. Department of Agriculture Forest Service Institute of Pacific Islands Forestry and NSF EPSCoR Awards 0237065 and 0554657. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of NSF. Manuscript accepted 15 September 2014. NR 42 TC 1 Z9 1 U1 3 U2 12 PU UNIV HAWAII PRESS PI HONOLULU PA 2840 KOLOWALU ST, HONOLULU, HI 96822 USA SN 0030-8870 EI 1534-6188 J9 PAC SCI JI Pac. Sci. PD JUL PY 2015 VL 69 IS 3 BP 367 EP 383 DI 10.2984/69.3.6 PG 17 WC Marine & Freshwater Biology; Zoology SC Marine & Freshwater Biology; Zoology GA CR3LP UT WOS:000361234500006 ER PT J AU Panyushkina, IP Leavitt, SW Domack, EW Wiedenhoeft, AC AF Panyushkina, Irina P. Leavitt, Steven W. Domack, Eugene W. Wiedenhoeft, Alex C. TI TREE-RING INVESTIGATION OF HOLOCENE FLOOD-DEPOSITED WOOD FROM THE ONEIDA LAKE WATERSHED, NEW YORK STATE SO TREE-RING RESEARCH LA English DT Article DE paleoflood; paleohydrology; riparian forest; US Northeast; Fish Creek; dendrochronology ID EASTERN NORTH-AMERICA; ENVIRONMENTAL-CHANGE; CLIMATE-CHANGE; EVENTS; OSCILLATION; VEGETATION; REGION; RIVER AB Glacial deposition and fluvial/lacustrine sedimentation interact over terrains in central New York State to preserve a history of geological and hydrological events as well as hydroclimatic transitions. The lower reach of Fish Creek draining the eastern watershed of Oneida Lake, NY, is an area with prominent wood remains. This study explores a collection of 52 logs encased in organic-rich deposits exposed by bank erosion at three locations along Fish Creek near Sylvan Beach, NY, with respect to radiocarbon ages, species, and the crossdating potential of tree rings. Radiocarbon ages and successful tree-ring crossdating document what we interpret as seven major hydrologic episodes ca. 10 ka (i.e. ca. 10,000 cal yr BP), 7.4 ka, 6.8 ka, 6.4 ka, 5.5 ka, 3.1 ka and 2.2 ka cal BP, during which channel aggradation and tree burial may have been associated with abruptly increased flood frequency and/or high water tables. This pilot study establishes four floating tree-ring records: [1] early Holocene hemlock (Tsuga), mid-Holocene [2] walnut (Juglans sp.) and [3] sycamore (Platanus), and [4] late Holocene elm (Ulmus sp.), with sample sizes of 8-14 series of 55-135 years length. Despite the complexity of distribution of radiocarbon ages at each site, the wealth of well-preserved wood demonstrates great promise for understanding the paleoflood history of the Oneida watershed by documenting the magnitude, location, and timing of floods. Further additional systematic sampling can add and strengthen tree-ring dating and tree-ring based flood records, confirm results, and contribute to the Holocene hydrological history of the region. C1 [Panyushkina, Irina P.; Leavitt, Steven W.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Domack, Eugene W.] Hamilton Coll, Dept Geosci, Clinton, NY 13323 USA. [Domack, Eugene W.] Univ S Florida, Coll Marine Sci, St Petersburg, FL 33701 USA. [Wiedenhoeft, Alex C.] USDA Forest Prod Lab, Ctr Wood Anat Res, Madison, WI 53726 USA. RP Panyushkina, IP (reprint author), Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. EM ipanyush@email.arizona.edu FU National Science Foundation, P2C2 Program [AGS-1003483] FX We are thankful to C. Griggs, N. Pederson and D. Barkley for their helpful comments and discussion of an early version of the manuscript. The thoughtful comments of two reviewers and Associate Editor G. Wiles greatly helped in improving the final paper. The radiocarbon dating was done at the NSF-Arizona Accelerator Mass Spectrometry (AMS) Laboratory at the University of Arizona. This research was sponsored by the National Science Foundation, P2C2 Program award #AGS-1003483. NR 45 TC 0 Z9 0 U1 4 U2 11 PU TREE-RING SOC PI TUCSON PA UNIV ARIZONA, TREE-RING LABORATORY, BLDG 58, TUCSON, AZ 85721 USA SN 1536-1098 EI 2162-4585 J9 TREE-RING RES JI Tree-Ring Res. PD JUL PY 2015 VL 71 IS 2 BP 83 EP 94 DI 10.3959/1536-1098-71.2.83 PG 12 WC Forestry SC Forestry GA CR3QT UT WOS:000361248700004 ER PT J AU Mmbaga, MT Kim, MS Mackasmiel, L Klopfenstein, NB AF Mmbaga, M. T. Kim, M. -S. Mackasmiel, L. Klopfenstein, N. B. TI Differentiation of Corynespora cassiicola and Cercospora sp in leaf-spot diseases of Hydrangea macrophylla using a PCR-mediated method SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Cercospora leaf spot; garden hydrangea; molecular diagnostics; plant disease complex; diagnostic primers AB Corynespora cassiicola and Cercospora sp. have been identified as the most prevalent and destructive leaf-spot pathogens of garden hydrangea [Hydrangea macrophylla (Thunberg) Seringe] in the southeastern USA, but they are often difficult to accurately detect and distinguish because they often occur together in a disease complex with other pathogenic leaf-spot fungi and produce very similar symptoms. This study was conducted to provide diagnostic PCR primers for detecting and distinguishing Corynespora cassiicola and Cercospora sp. among other leaf-spot pathogens of garden hydrangea. Two primer pairs showed specificity to Corynespora cassiicola and one primer pair showed specificity to Cercospora sp., and these primers did not amplify DNA from any other common fungal pathogens associated with hydrangea leaf-spot diseases. Results from this study show that DNA-based diagnostic primers provide a useful tool for pathogen detection/identification in hydrangea leaf-spot disease, which is an essential step toward understanding disease etiology and developing/applying appropriate disease-management practices in the southeastern USA. C1 [Mmbaga, M. T.; Mackasmiel, L.] Tennessee State Univ, Sch Agr & Consumer Sci, Otis A Floyd Nursery Res Ctr, Mcminnville, TN 37110 USA. [Kim, M. -S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul 136702, South Korea. [Klopfenstein, N. B.] US Forest Serv, USDA, RMRS, Moscow, ID 83843 USA. RP Mmbaga, MT (reprint author), Tennessee State Univ, Sch Agr & Consumer Sci, Otis A Floyd Nursery Res Ctr, 472 Cadillac Lane, Mcminnville, TN 37110 USA. EM mkim@kookmin.ac.kr NR 21 TC 0 Z9 0 U1 2 U2 5 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD JUL PY 2015 VL 95 IS 4 BP 711 EP 717 DI 10.4141/CJPS-2014-354 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CR0FP UT WOS:000360994100011 ER PT J AU Jego, G Rotz, CA Belanger, G Tremblay, GF Charbonneau, E Pellerin, D AF Jego, Guillaume Rotz, C. Alan Belanger, Gilles Tremblay, Gaetan F. Charbonneau, Edith Pellerin, Doris TI Simulating forage crop production in a northern climate with the Integrated Farm System Model SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Integrated Farm System Model; timothy; alfalfa; mixture; dairy farm; Canada ID DAIRY PRODUCTION SYSTEMS; NITROUS-OXIDE EMISSIONS; GLOBAL ACTIVITY MODEL; SNOW-COVER MODEL; NUTRITIVE-VALUE; TIMOTHY YIELD; CATIMO MODEL; GROWTH ANALYSIS; EASTERN CANADA; NEW-ZEALAND AB Whole-farm simulation models are useful tools for evaluating the effect of management practices and climate variability on the agro-environmental and economic performance of farms. A few process-based farm-scale models have been developed, but none has been evaluated in northern regions with boreal and hemiboreal climates characterized by a short growing season and a long period with snow cover. The study objectives were to calibrate the grass sub-model of the Integrated Farm System Model (IFSM) and evaluate its predictions of yield and nutritive value of timothy and alfalfa, grown alone or in a mixture, using experimental field data from across Canada, and to assess IFSM's predictions of yield of major annual crops grown on dairy farms in eastern Canada using regional yield data from two contrasting regions. Several timothy and alfalfa datasets combining sites, years, harvests, and N fertilization rates were used to calibrate and evaluate the model. For timothy and alfalfa, the model's accuracy was globally satisfactory in predicting dry matter yield and neutral detergent fiber concentration with a normalized root mean square error (NRMSE) <30%. For N uptake, the scatter was a bit larger, especially for timothy (NRMSE = 49%), mainly because of a small range in the measured data. The model's accuracy for predicting the yield of annual crops was generally good, with an NRMSE <30%. Adding timothy and alfalfa to the grass sub-model of IFSM and verifying the model's performance for annual crops confirmed that IFSM can be used in northern regions of North America. In addition, the model was able to simulate the yield and nutritive value of a timothy-alfalfa mixture, which is the most common perennial mixture used in Canada. C1 [Jego, Guillaume; Belanger, Gilles; Tremblay, Gaetan F.] Agr & Agri Food Canada, Soils & Crops Res & Dev Ctr, Quebec City, PQ G1V 2J3, Canada. [Rotz, C. Alan] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Charbonneau, Edith; Pellerin, Doris] Univ Laval, Dept Anim Sci, Quebec City, PQ G1V 0A6, Canada. RP Jego, G (reprint author), Agr & Agri Food Canada, Soils & Crops Res & Dev Ctr, 2560 Hochelaga Blvd, Quebec City, PQ G1V 2J3, Canada. EM guillaume.jego@agr.gc.ca FU Agriculture and Agri-Food Canada; Canadian Dairy Research Cluster FX The authors thank Dr. Philippe Savoie for making this collaborative work possible, Dr. Vern Baron for providing the Lacombe dataset, Juan-Manuel Moreno-Prado for processing the regional yield data, various research assistants, and the students who assisted with the field and laboratory measurements and data handling at the sites. This work was funded by Agriculture and Agri-Food Canada and the Canadian Dairy Research Cluster. NR 62 TC 1 Z9 1 U1 6 U2 19 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD JUL PY 2015 VL 95 IS 4 BP 745 EP 757 DI 10.4141/CJPS-2014-375 PG 13 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CR0FP UT WOS:000360994100015 ER PT J AU Kirn, DR Koochek, A Reid, KF von Berens, A Travison, TG Folta, S Sacheck, J Nelson, M Liu, C Phillips, E Aberg, AC Nydahl, M Gustafsson, T Cederholm, T Fielding, RA AF Kirn, Dylan R. Koochek, Afsaneh Reid, Kieran F. von Berens, Asa Travison, Thomas G. Folta, Sara Sacheck, Jennifer Nelson, Miriam Liu, Christine Phillips, Edward Aberg, Anna Cristina Nydahl, Margaretha Gustafsson, Thomas Cederholm, Tommy Fielding, Roger A. TI The Vitality, Independence, and Vigor in the Elderly 2 Study (VIVE2): Design and methods SO CONTEMPORARY CLINICAL TRIALS LA English DT Article DE Older adults; Mobility-limitations; Physical activity; Protein; Vitamin D; Supplementation ID RANDOMIZED CONTROLLED-TRIAL; LOWER-EXTREMITY FUNCTION; PHYSICAL PERFORMANCE BATTERY; VITAMIN-D SUPPLEMENTATION; PLACEBO-CONTROLLED TRIAL; MUSCLE PROTEIN-SYNTHESIS; LIMITED OLDER-ADULTS; SKELETAL-MUSCLE; RESISTANCE EXERCISE; BODY-COMPOSITION AB Background: Nutritional supplementation may potentiate the increase in skeletal muscle protein synthesis following exercise in healthy older individuals. Whether exercise and nutrition act synergistically to produce sustained changes in physical functioning and body composition has not been well studied, particularly in mobility-limited older adults. Methods: The VIVE2 study was a multi-center, randomized controlled trial, conducted in the United States and Sweden. This study was designed to compare the effects of a 6-month intervention with a once daily, experimental, 4 fl. oz. liquid nutritional supplement providing 150 kcal, whey protein (20 g), and vitamin D (800 IU) (Nestle Health Science, Vevey, Switzerland), to a low calorie placebo drink (30 kcal, non-nutritive; identical format) when combined with group-based exercise in 150 community-dwelling, mobility-limited older adults. All participants participated in a structured exercise program (3 sessions/week for 6 months), which included aerobic, strength, flexibility, and balance exercises. Results: The primary outcome was 6-month change in 400 m walk performance (m/s) between supplement and placebo groups. Secondary outcomes included 6 month change in: body composition, muscle cross-sectional area, leg strength, grip strength, stair climb time, quality of life, physical performance, mood/depressive symptoms and nutritional status. These outcomes were selected based on their applicability to the health and wellbeing of older adults. Conclusions: The results of this study will further define the role of nutritional supplementation on physical functioning and restoration of skeletal muscle mass in older adults. Additionally, these results will help refine the current physical activity and nutritional recommendations for mobility-limited older adults. (C) 2015 Elsevier Inc. All rights reserved. C1 [Kirn, Dylan R.; Reid, Kieran F.; Liu, Christine; Phillips, Edward; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Koochek, Afsaneh; von Berens, Asa; Cederholm, Tommy] Uppsala Univ, Dept Publ Hlth & Caring Sci, Clin Nutr & Metab, Uppsala, Sweden. [Gustafsson, Thomas] Karolinska Inst, Dept Lab Med, Stockholm, Sweden. [Folta, Sara; Sacheck, Jennifer; Nelson, Miriam] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Nelson, Miriam] Tufts Univ, Jonathan M Tisch Coll Citizenship & Publ Serv, Medford, MA 02155 USA. [Travison, Thomas G.] Hebrew SeniorLife, Inst Aging Res, Boston, MA USA. [Travison, Thomas G.; Phillips, Edward] Harvard Univ, Sch Med, Boston, MA USA. [Phillips, Edward] Spaulding Rehabil Hosp, Boston, MA USA. [Nydahl, Margaretha] Boston Univ, Sch Med, Sect Geriatr, Boston, MA 02118 USA. [Aberg, Anna Cristina] Uppsala Univ, Dept Publ Hlth & Caring Sci, Geriatr, Uppsala, Sweden. [Aberg, Anna Cristina] Dalarna Univ, Sch Educ Hlth & Soc, Dalarna, Sweden. [Liu, Christine] Uppsala Univ, Dept Food Nutr & Dietet, Uppsala, Sweden. [Travison, Thomas G.] Beth Israel Deaconess Med Ctr, Div Gerontol, Boston, MA 02215 USA. RP Kirn, DR (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Dylan.kim@tufts.edu FU Nestle Health Science, Vevey, Switzerland (CTA) [10.27.CLI]; U.S. Department of Agriculture [58-1950-0-014] FX This work was supported by Nestle Health Science, Vevey, Switzerland (CTA# 10.27.CLI).; This study was supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 72 TC 2 Z9 2 U1 1 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1551-7144 EI 1559-2030 J9 CONTEMP CLIN TRIALS JI Contemp. Clin. Trials PD JUL PY 2015 VL 43 BP 164 EP 171 DI 10.1016/j.cct.2015.06.001 PG 8 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA CQ7HI UT WOS:000360773200021 PM 26044464 ER PT J AU Warburton, ML Diepenbrock, CH AF Warburton, Marilyn L. Diepenbrock, Christine H. TI Introduction to the QTL That Matter Symposium SO CROP SCIENCE LA English DT Editorial Material C1 [Warburton, Marilyn L.] USDA ARS, Res Genet, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Diepenbrock, Christine H.] Cornell Univ, Plant Breeding & Genet Sect, Sch Integrat Plant Sci, Ithaca, NY 14850 USA. RP Warburton, ML (reprint author), USDA ARS, Res Genet, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. EM marilyn.warburton@ars.usda.gov NR 0 TC 0 Z9 0 U1 3 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2015 VL 55 IS 4 BP 1435 EP 1436 DI 10.2135/cropsci2015.04.0001in PG 2 WC Agronomy SC Agriculture GA CQ9MH UT WOS:000360938600003 ER PT J AU Semagn, K Beyene, Y Babu, R Nair, S Gowda, M Das, B Tarekegne, A Mugo, S Mahuku, G Worku, M Warburton, ML Olsen, M Prasanna, BM AF Semagn, Kassa Beyene, Yoseph Babu, Raman Nair, Sudha Gowda, Manje Das, Biswanath Tarekegne, Amsal Mugo, Stephen Mahuku, George Worku, Mosisa Warburton, Marilyn L. Olsen, Mike Prasanna, B. M. TI Quantitative Trait Loci Mapping and Molecular Breeding for Developing Stress Resilient Maize for Sub-Saharan Africa SO CROP SCIENCE LA English DT Article ID SINGLE NUCLEOTIDE POLYMORPHISMS; WELL-WATERED ENVIRONMENTS; ANTHESIS-SILKING INTERVAL; CHLOROTIC-MOTTLE-VIRUS; DROUGHT TOLERANCE; TROPICAL MAIZE; POPULATIONS REVEALS; SECONDARY TRAITS; MASCARENE SOURCE; LETHAL NECROSIS AB The International Maize and Wheat Improvement Center (CIMMYT), in partnership with several public and private institutions, is working to develop and deploy improved maize (Zea mays L.) germplasm that is drought tolerant, nitrogen use efficient (NUE), and disease resistant for sub-Saharan Africa (SSA), using conventional pedigree selection and molecular breeding. Here, we provide an overview of the progress made on (i) quantitative trait loci (QTL) analysis for drought, NUE, and maize lethal necrosis (MLN); (ii) development of production markers for maize streak virus (MSV) and MLN resistance; and (iii) marker-assisted recurrent selection (MARS) and genomic selection (GS) for developing drought tolerant maize germplasm. We identified several small to moderate effect QTL associated with grain yield and anthesis-silking interval under low N, managed drought, and optimum environments, but only a few small to moderate effect QTL were detected in multiple genetic backgrounds. Thus, CIMMYT is conducting the largest public MARS and GS projects in SSA. A recent comparative study of pedigree selection, MARS and GS undertaken across 8 to 10 bi-parental populations, demonstrated the superior performance of both MARS and GS schemes over pedigree selection. More than 90% of current commercial maize germplasm was found to be highly susceptible to MLN disease. Preliminary mapping studies identified three major QTL for MLN disease resistance, and two are being used for pilot line conversion using marker-assisted backcrossing (MABC). For MSV, pilot marker-assisted selection is ongoing, using three single nucleotide polymorphic (SNP) markers to select a major QTL on chromosome 1. Development of drought tolerant and NUE maize varieties must include MLN resistance in eastern Africa, and we strongly suggest to maize breeders to introgress promising genomic regions for MLN resistance using either conventional or molecular methods. C1 [Semagn, Kassa; Beyene, Yoseph; Gowda, Manje; Das, Biswanath; Mugo, Stephen; Mahuku, George; Worku, Mosisa; Olsen, Mike; Prasanna, B. M.] Int Maize & Wheat Improvement Ctr CIMMYT, Nairobi 00621, Kenya. [Babu, Raman; Nair, Sudha] Int Crops Res Inst Semi Arid Trop, CIMMYT Asian Maize Program, Patancheru 502324, Andhra Pradesh, India. [Tarekegne, Amsal] CIMMYT, Harare, Zimbabwe. [Warburton, Marilyn L.] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. RP Semagn, K (reprint author), Int Maize & Wheat Improvement Ctr CIMMYT, POB 1041, Nairobi 00621, Kenya. EM k.semagn@cgiar.org OI Semagn, Kassa/0000-0001-6486-5685 FU Bill & Melinda Gates Foundation; Howard Buffet Foundation; United States Agency for International Development (USAID); Syngenta Foundation for Sustainable Agriculture; CGIAR Research Program in Maize FX We are grateful to CIMMYT Field Technicians at the different stations in Kenya, Zimbabwe, and Zambia for phenotypic evaluation of the populations; CIMMYT Laboratory Technicians in Kenya for sample preparation for genotyping; the Monsanto Company for genotyping the WEMA populations and also providing marker information. The work presented here was carried mainly under the DTMA, WEMA, IMAS, and MLN-Africa projects funded by the Bill & Melinda Gates Foundation, Howard Buffet Foundation, United States Agency for International Development (USAID), Syngenta Foundation for Sustainable Agriculture, and the CGIAR Research Program in Maize. NR 48 TC 3 Z9 3 U1 4 U2 25 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2015 VL 55 IS 4 BP 1449 EP 1459 DI 10.2135/cropsci2014.09.0646 PG 11 WC Agronomy SC Agriculture GA CQ9MH UT WOS:000360938600005 ER PT J AU Pinson, SRM Wang, YG Tabien, RE AF Pinson, Shannon R. M. Wang, Yueguang Tabien, Rodante E. TI Mapping and Validation of Quantitative Trait Loci Associated with Tiller Production in Rice SO CROP SCIENCE LA English DT Article ID ORYZA-SATIVA L.; YIELD-RELATED TRAITS; RECOMBINANT INBRED LINES; AGRONOMIC TRAITS; BLIGHT RESISTANCE; FIELD-RESISTANCE; HEADING DATE; GRAIN-YIELD; QTLS; GENE AB An increase in early tiller production is desired in rice (Oryza sativa L.) to increase yield potential and enhance ability to shade and suppress weeds. Unfortunately, tiller production and survival are sensitive to many environmental cues, making tillering pattern a difficult trait to reliably evaluate in field plots. The present objective was to use pot-grown plants where seeding depth and the environment could be controlled to identify quantitative trait loci (QTLs) associated with rate of seedling development (seedling leaf number, SLN) and tiller production (tiller number, TN) as well as the node from which the first tiller originated (N1T). The QTLs were identified in two related mapping populations, the first being a set of 280 'Lemont'. 'TeQing' recombinant inbred lines (RILs) observed over four trials, in which nine QTLs associated with TN, three for SLN, and two for N1T were detected. Eight of the TN QTLs were verified in a second population consisting of 123 TeQing-into-Lemont backcross introgression lines (TILs). Each SLN and N1T QTL was colocated with a TN QTL, and all nine detected TN QTLs were located in genomic regions previously reported to contain TN major genes or QTLs, demonstrating their robustness under diverse genetic backgrounds and growth conditions. Early tillering was associated with rapid seedling development as detected by an increased number of leaves and nodes on 8-wk-old seedlings. The present data indicated that breeders could effectively select for enhanced early tiller production among segregating genotypes based on tiller counts conducted at a single time point approximately 8 to 9 wk after germination under greenhouse conditions. Marker-trait linkages reported here can support marker-assisted selection of these nine TN QTLs. C1 [Pinson, Shannon R. M.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Wang, Yueguang] Univ Idaho, Moscow, ID 83843 USA. [Tabien, Rodante E.] Texas A&M Agrilife Res, Beaumont, TX USA. RP Pinson, SRM (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130 East, Stuttgart, AR 72160 USA. EM shannon.pinson@ars.usda.gov FU Texas Rice Research Foundation FX This research was supported in part by a grant from the Texas Rice Research Foundation. The authors acknowledge Dr. Kathleen Yeater for consultation on analyzing marker-trait associations using SAS JMP Genomics and acknowledge Ms. Faye Seaberg and Ms. Piper Roberts whose expertise and dedication in monitoring plant growth details were essential to this study. NR 78 TC 2 Z9 2 U1 1 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2015 VL 55 IS 4 BP 1537 EP 1551 DI 10.2135/cropsci2014.09.0644 PG 15 WC Agronomy SC Agriculture GA CQ9MH UT WOS:000360938600011 ER PT J AU Trapp, JJ Urrea, CA Cregan, PB Miklas, PN AF Trapp, Jennifer J. Urrea, Carlos A. Cregan, Perry B. Miklas, Phillip N. TI Quantitative Trait Loci for Yield under Multiple Stress and Drought Conditions in a Dry Bean Population SO CROP SCIENCE LA English DT Article ID PHASEOLUS-VULGARIS L.; MYB TRANSCRIPTION FACTOR; PHOTOPERIOD SENSITIVITY; QTL-ANALYSIS; GRAIN-YIELD; COMMON; RESISTANCE; TOLERANCE; CULTIVARS; GENES AB Terminal and intermittent drought limits dry bean (Phaseolus vulgaris L.) production worldwide. Tolerance to drought exists but is difficult to breed for because of inconsistent expression across environments. Our objective was to identify quantitative trait loci (QTL) conditioning yield in a recombinant inbred line (RIL) population with consistent expression across multiple drought-stress environments. We tested 140 RILs from 'Buster' pinto (susceptible)/'Roza' pink (tolerant) for yield under multiple stresses (intermittent drought, compaction, and low fertility) across 3 yr and terminal drought across four location-years. A genetic linkage map (953 cM) was generated using single nucleotide polymorphism (SNP) markers. Two major-effect QTL were detected on Pv01 and Pv02. The Pv01 QTL, defined by the closest marker SNP50809 (47.7 Mb), explained up to 37% of the phenotypic variance for seed yield under multiple stress (including intermittent drought) and was consistently expressed each year. The Pv02 QTL, nearest SNP40055 (11.8 Mb), was detected under drought stress (R-2 = 33%) in addition to multiple stress (R-2 = 17-23%). Phenological traits cosegregated with the yield QTL and affirmed the importance of phenological plasticity in adaptation to drought stress. Late maturity contributed to increased yield under multiple and nonstress and early maturity to increased yield under terminal drought. Given major and consistent effect, further investigation of the potential for the Pv01 and Pv02 QTL in breeding for multiple abiotic stress and drought tolerance in dry bean is warranted. C1 [Trapp, Jennifer J.; Miklas, Phillip N.] USDA ARS, Grain Legume Genet & Physiol Res Unit, Prosser, WA 99350 USA. [Urrea, Carlos A.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, BARC West, Beltsville, MD 20705 USA. RP Miklas, PN (reprint author), USDA ARS, Grain Legume Genet & Physiol Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM phil.miklas@ars.usda.gov FU Norman Borlaug Commemorative Research Initiative [58-0210-3-012 (408-5354-012)] FX We thank Drs. Troy Peters and Prossie Nakawuka, Washington State University, for their assistance with soil water measurement and management and Dr. Kim Campbell for assistance with statistical analyses. This work was supported, in part, by funding received from the Norman Borlaug Commemorative Research Initiative between US Agency for International Development and the USDA-ARS through Cooperative Agreement Number 58-0210-3-012 (408-5354-012). NR 49 TC 4 Z9 4 U1 3 U2 12 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2015 VL 55 IS 4 BP 1596 EP 1607 DI 10.2135/cropsci2014.11.0792 PG 12 WC Agronomy SC Agriculture GA CQ9MH UT WOS:000360938600016 ER PT J AU Burson, BL Renganayaki, K Dowling, CD Hinze, LL Jessup, RW AF Burson, Byron L. Renganayaki, K. Dowling, Charlie D. Hinze, Lori L. Jessup, Russell W. TI Genetic Diversity among Pentaploid Buffelgrass Accessions SO CROP SCIENCE LA English DT Article ID CENCHRUS-CILIARIS POACEAE; APOMICTIC BUFFELGRASS; FACULTATIVE APOMIXIS; PENNISETUM-CILIARE; GRASS ACCESSIONS; REGISTRATION; PANICOIDEAE; GENOTYPES; MARKERS; FERTILIZATION AB Buffelgrass [Pennisetum ciliare (L.) Link (syn. Cenchrus ciliaris L.)], an important pasture and range grass in the arid semi-tropics and tropics, has excellent drought-tolerance but lacks winter hardiness. It is a polymorphic, apomictic species and its most common chromosome number is 2n = 4x = 36. Eighty-six pentaploid (2n = 5x = 45) accessions from South Africa were deposited into the USDA National Germplasm System (NPGS) in 1976 and these were more winter-hardy than all tetraploid accessions. Prior to 1976, only 31 pentaploids were in the NPGS but they lacked cold tolerance. Since 1976, ten pentaploid accessions with unknown cold tolerance have been added to the NPGS and they were included. The genetic diversity among all these pentaploid accessions was investigated to determine if the winter-hardy and non-winter-hardy genotypes were distinct. Amplified fragment length polymorphisms were used to determine the genetic diversity and phylogenetic relationships among the accessions. Ten primer combinations generated 862 polymorphic bands; the number of bands identified by each primer ranged from 74 to 117 (mean 93.4). Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis and principal coordinate analysis revealed two groups with one separating into two sub-groups and one sub-group separating into two subdivisions. Genetic similarity coefficients ranged from 0.39 to 0.98 (mean 0.74). These findings show considerable genetic diversity among the pentaploid accessions. Most cold-tolerant and non-cold-tolerant genotypes separated by subgroups but not by group. Nine tetraploid accessions, included for reference, clustered into several subgroups. The pentaploid genotypes appear to have polyphyletic origins. C1 [Burson, Byron L.; Hinze, Lori L.] USDA ARS, Crop Germplasm Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Renganayaki, K.; Dowling, Charlie D.; Jessup, Russell W.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. RP Burson, BL (reprint author), USDA ARS, Crop Germplasm Res Unit, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. EM byron.burson@ars.usda.gov OI Hinze, Lori L/0000-0003-3356-1245 NR 54 TC 0 Z9 0 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2015 VL 55 IS 4 BP 1637 EP 1645 DI 10.2135/cropsci2014.09.0655 PG 9 WC Agronomy SC Agriculture GA CQ9MH UT WOS:000360938600020 ER PT J AU Mishra, S Heckathorn, S Krause, C AF Mishra, Sasmita Heckathorn, Scott Krause, Charles TI The Levels of Boron-Uptake Proteins in Roots are Correlated with Tolerance to Boron Stress in Barley SO CROP SCIENCE LA English DT Article ID TOXICITY TOLERANCE; RHAMNOGALACTURONAN II; ARABIDOPSIS-THALIANA; HIGHER-PLANTS; DEFICIENCY; TRANSPORTER; EXPRESSION; WHEAT; EFFLUX; AVAILABILITY AB Boron (B) is an essential micronutrient required for plant growth and development. Recently, two major B-uptake proteins, BOR 1 and NIP5; 1, have been identified and partially characterized. BOR 1 is a high-affinity B transporter involved in xylem loading in roots, and NIP5; 1 is a major boric-acid channel in the plasma membrane. The aim of the present study was to determine if plant tolerance to B stress (deficiency or toxicity) is correlated with natural levels of B-uptake proteins in roots. We grew two barley (Hordeum vulgare L.) cultivars that differ in tolerance to low and high B (Schooner and Clipper, respectively) at three B levels (sub-, near-, and supraoptimal), and then we determined the concentration of BOR 1 and NIP5; 1 in roots using enzyme-linked immunosorbent assay (ELISA) and protein-specific antibodies that we developed. Shoot and root biomass in Schooner decreased at high B, while shoot mass in Clipper decreased in low B. Differences between cultivars in tolerance to B stress were unrelated to the concentration of B in plant tissue or to effects of B on root total protein concentration. BOR 1 content per unit total protein and per gram of root was greater in Clipper than Schooner, while NIP5; 1 content was greater in Schooner. The estimated activity of BOR 1 (B uptake per BOR 1) did not differ between cultivars. These results show that whole-plant tolerance to B deficiency and toxicity is correlated with levels of the major B-uptake proteins in roots (BOR 1 and NIP5; 1). Hence, BOR 1 and NIP5; 1 can potentially be used as biomarkers to identify plant genotypes with enhanced tolerance to B stress (e.g., for agriculture or phytoremediation). C1 [Mishra, Sasmita; Heckathorn, Scott] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Krause, Charles] Univ Toledo, USDA, Toledo, OH 43606 USA. RP Mishra, S (reprint author), Univ Toledo, Dept Environm Sci, 2801 W Bancroft St, Toledo, OH 43606 USA. EM sasmita.mishra@utoledo.edu FU Ohio Plant Biotechnology Consortium; USDA [SCA 58-3607-9-741] FX This research was supported by the Ohio Plant Biotechnology Consortium and USDA (SCA 58-3607-9-741). We thank Douglas Sturtz, Russ Friedrich, and the USDA for nutrient analysis; Dr. Harold Bockelman and Dr. Tim Sutton for providing us the seeds of different barley cultivars; and the anonymous reviewers for helpful comments. NR 44 TC 1 Z9 1 U1 3 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2015 VL 55 IS 4 BP 1741 EP 1748 DI 10.2135/cropsci2014.10.0706 PG 8 WC Agronomy SC Agriculture GA CQ9MH UT WOS:000360938600029 ER PT J AU Dunlap, CA Kim, SJ Kwon, SW Rooney, AP AF Dunlap, Christopher A. Kim, Soo-Jin Kwon, Soon-Wo Rooney, Alejandro P. TI Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp plantarum is a later heterotypic synonym of Bacillus methylotrophicus SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID COMPLETE GENOME SEQUENCE; DNA-DNA HYBRIDIZATION; FUSARIUM HEAD BLIGHT; GAMMA-GLUTAMIC ACID; GENE-CLUSTER; STRAIN; GROWTH; BIOCONTROL; SUBTILIS; ANTAGONISTS AB The rhizosphere-isolated bacteria belonging to the Bacillus amyloliquefaciens subsp. plantarum and Bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. These properties have made these strains the focus of commercial interest. Here, we present the draft genome sequence of B. methylotrophicus KACC 13105(T) (=CBMB205(T)). Comparative genomic analysis showed only minor differences between this strain and the genome of the B. amyloliquefaciens subsp. plantarum type strain, with the genomes sharing approximately 95% of the same genes. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the type strains of these two taxa are highly similar. In fact, our results show that the type strain of B. amyloliquefaciens subsp. plantarum FZB42(T) (=DSM 23117(T)=BGSC 10A6(T)) does not cluster with other members of the B. amyloliquefaciens taxon. Instead, it clusters well within a clade of strains that are assigned to B. methylotrophicus, including the type strain of that species. Therefore, we propose that the subspecies B. amyloliquefaciens subsp. plantarum should be reclassified as a later heterotypic synonym of B. methylotrophicus. C1 [Dunlap, Christopher A.; Kim, Soo-Jin; Rooney, Alejandro P.] USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Units, Peoria, IL 61604 USA. [Kim, Soo-Jin; Kwon, Soon-Wo] KACC, Agr Microbiol Div, Natl Acad Agr Sci, Rural Dev Adm, Wanju Gun, Jeollabuk Do, South Korea. RP Dunlap, CA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Units, 1815 N Univ St, Peoria, IL 61604 USA. EM christopher.dunlap@ars.usda.gov FU National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ011248] FX This study was supported by the National Academy of Agricultural Science, Rural Development Administration, Republic of Korea 'Research Program for Agricultural Science & Technology Development (project no. PJ011248)'. The authors would like to thank Heather Walker for expert technical assistance. In addition, the authors would like to thank Dr Rudiger Pukall of the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, for critical review and comments. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture (USDA). The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. NR 33 TC 16 Z9 17 U1 4 U2 18 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUL PY 2015 VL 65 BP 2104 EP 2109 DI 10.1099/ijs.0.000226 PN 7 PG 6 WC Microbiology SC Microbiology GA CQ8QN UT WOS:000360873600013 PM 25835027 ER PT J AU Dunlap, CA AF Dunlap, Christopher A. TI The status of the species Bacillus aerius. Request for an Opinion SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID SP-NOV.; AEROPHILUS AB During a recent study assessing the diversity of the species Bacillus licheniformis, it became apparent that the type strain of Bacillus aerius was not available from any established culture collection or from the authors who originally described it. Other authors have reported similar findings when trying to obtain the strain (Lai et al., 2014), therefore, this species cannot currently be included in any further scientific studies. It is proposed that, if suitable replacements for type strains are not found or neotype strains,are not proposed within two years following the publication of this Request for an Opinion, the Judicial Commission of the International Committee of Systematics of Prokaryotes place the name B. aerius on the list of rejected names. C1 USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. RP Dunlap, CA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM christopher.dunlap@ars.usda.gov NR 4 TC 1 Z9 1 U1 2 U2 3 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUL PY 2015 VL 65 BP 2341 EP 2341 DI 10.1099/ijs.0.000271 PN 7 PG 1 WC Microbiology SC Microbiology GA CQ8QN UT WOS:000360873600051 PM 25908707 ER PT J AU Spurgeon, DW Brent, CS AF Spurgeon, D. W. Brent, C. S. TI Diapause Response to Photoperiod in an Arizona Population of Lygus hesperus (Hemiptera: Miridae) SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Lygus hesperus; western tarnished plant bug; photoperiod; diapause ID PLANT BUG HETEROPTERA; REPRODUCTIVE DIAPAUSE; KNIGHT AB The western tarnished plant bug (Lygus hesperus Knight) is an important crop pest in the western United States that overwinters in an adult diapause. However, knowledge of L. hesperus diapause is incomplete. Eggs from field-collected adults were reared under photoperiods of 10:14, 11:13, 12:12, and 13:11 (L:D) h at 26.7 +/- 1 degrees C, and the diapause response for each gender was modeled by a logistic function. Incidence of a recently reported "glass bead" fat body type also was examined. Validation studies using the same methods were subsequently conducted using photoperiods of 10.5:13.5, 11.5:12.5, and 12.5:11.5 (L:D) h. No effects of bug gender, photoperiod, or diapause status on occurrence of the novel fat type were detected. Estimates of diapause in validation studies were within confidence intervals for initial predictions, but systematic deviations from initial predictions prompted refitting of the models to include validation data. Refitted functions estimated critical photoperiods of 11h44min for females and 11h21min for males. The maximum incidence of diapause was lower for males than for females and was <100% for both genders. Refitted functions also predicted 50% of the population-specific maximum diapause response corresponded to photoperiods of 11h54min (females) and 12h7min (males). These results, combined with other recent findings, suggest heterogeneity in diapause response likely enables L. hesperus populations to adapt to local conditions. The estimated functions relating photoperiod to diapause incidence provide baselines to facilitate future studies of environmental and geographical influences on diapause in this species. C1 [Spurgeon, D. W.; Brent, C. S.] USDA, ARS, Pest Management & Biocontrol Res Unit, Maricopa, AZ 85138 USA. RP Spurgeon, DW (reprint author), USDA, ARS, Pest Management & Biocontrol Res Unit, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. EM Dale.Spurgeon@ars.usda.gov NR 17 TC 1 Z9 1 U1 1 U2 2 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2015 VL 50 IS 3 BP 238 EP 247 PG 10 WC Entomology SC Entomology GA CR1IW UT WOS:000361079400007 ER PT J AU Ni, XZ Rileys, DG Sparks, AN AF Ni, Xinzhi Rileys, David G. Sparks, Alton N., Jr. TI Aggregation and Foraging Behavior of Imported Cabbageworm (Lepidoptera: Pieridae) Adults on Blue Vervain Flowers SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE cabbage white butterfly; aggregation; foraging behavior; blue vervain C1 [Ni, Xinzhi; Rileys, David G.; Sparks, Alton N., Jr.] Univ Georgia, USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. RP Ni, XZ (reprint author), Univ Georgia, USDA ARS, Crop Genet & Breeding Res Unit, Tifton Campus, Tifton, GA 31793 USA. EM xinzhi.ni@ars.usda.gov NR 0 TC 0 Z9 0 U1 3 U2 3 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2015 VL 50 IS 3 BP 252 EP 253 PG 2 WC Entomology SC Entomology GA CR1IW UT WOS:000361079400009 ER PT J AU Jarvie, HP Sharpley, AN Flaten, D Kleinman, PJA Jenkins, A Simmons, T AF Jarvie, Helen P. Sharpley, Andrew N. Flaten, Don Kleinman, Peter J. A. Jenkins, Alan Simmons, Tarra TI The Pivotal Role of Phosphorus in a Resilient Water-Energy-Food Security Nexus SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID HARMFUL ALGAL BLOOMS; LAKE-ERIE; ECOSYSTEM SERVICES; LONG-TERM; AGRICULTURAL PHOSPHORUS; LIVESTOCK PRODUCTION; NONPOINT POLLUTION; RE-EUTROPHICATION; CANADIAN PRAIRIES; TILLAGE SYSTEMS AB We make the case that phosphorus (P) is inextricably linked to an increasingly fragile, interconnected, and interdependent nexus of water, energy, and food security and should be managed accordingly. Although there are many other drivers that influence water, energy, and food security, P plays a unique and under-recognized role within the nexus. The P paradox derives from fundamental challenges in meeting water, energy, and food security for a growing global population. We face simultaneous dilemmas of overcoming scarcity of P to sustain terrestrial food and biofuel production and addressing overabundance of P entering aquatic systems, which impairs water quality and aquatic ecosystems and threatens water security. Historical success in redistributing rock phosphate as fertilizer to enable modern feed and food production systems is a grand societal achievement in overcoming inequality. However, using the United States as the main example, we demonstrate how successes in redistribution of P and reorganization of farming systems have broken local P cycles and have inadvertently created instability that threatens resilience within the nexus. Furthermore, recent expansion of the biofuels sector is placing further pressure on P distribution and availability. Despite these challenges, opportunities exist to intensify and expand food and biofuel production through recycling and better management of land and water resources. Ultimately, a strategic approach to sustainable P management can help address the P paradox, minimize tradeoffs, and catalyze synergies to improve resilience among components of the water, energy, and food security nexus. C1 [Jarvie, Helen P.; Jenkins, Alan] Ctr Ecol & Hydrol, Wallingford OX10 8BB, Oxon, England. [Sharpley, Andrew N.; Simmons, Tarra] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Flaten, Don] Univ Manitoba, Dept Soil Sci, Winnipeg, MB R3T 2N2, Canada. [Kleinman, Peter J. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Jarvie, HP (reprint author), Ctr Ecol & Hydrol, Wallingford OX10 8BB, Oxon, England. EM hpj@ceh.ac.uk RI Jenkins, Alan/I-8742-2012 FU UK Natural Environment Research Council via the Macronutrient Cycles Thematic Programme [NE/J011991/1]; UK Natural Environment Research Council via National Capability Research funding (Impacts of Nutrient Management) [NEC04879] FX This work was supported by the UK Natural Environment Research Council via the Macronutrient Cycles Thematic Programme (grant NE/J011991/1) and via National Capability Research funding (Impacts of Nutrient Management; grant NEC04879). The authors thank Paul Fixen at the International Plant Nutrition Institute for providing access to, and advice on, the NuGIS dataset and Chris Wardle at the British Geological Survey for help with the artwork for Figure 1. NR 119 TC 9 Z9 9 U1 13 U2 84 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2015 VL 44 IS 4 BP 1049 EP 1062 DI 10.2134/jeq2015.01.0030 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ8JC UT WOS:000360852600003 PM 26437086 ER PT J AU Jacinthe, PA Vidon, P Fisher, K Liu, X Baker, ME AF Jacinthe, P. A. Vidon, P. Fisher, K. Liu, X. Baker, M. E. TI Soil Methane and Carbon Dioxide Fluxes from Cropland and Riparian Buffers in Different Hydrogeomorphic Settings SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NITROUS-OXIDE EMISSION; LAND-USE; MICROBIAL BIOMASS; US MIDWEST; RESPIRATION; GROUNDWATER; DYNAMICS; FOREST; VARIABILITY; PEATLAND AB Riparian buffers contribute to the mitigation of nutrient pollution in agricultural landscapes, but there is concern regarding their potential to be hot spots of greenhouse gas production. This study compared soil CO2 and CH4 fluxes in adjacent crop fields and riparian buffers (a flood-prone forest and a flood-protected grassland along an incised channel) and examined the impact of water table depth (WTD) and flood events on the variability of gas fluxes in riparian zones. Results showed significantly (P < 0.001) higher CO2 emission in riparian areas than in adjoining croplands (6.8 +/- 0.6 vs. 3.6 +/- 0.5 Mg CO2-C ha(-1) yr(-1); mean +/- SE). Daily flux of CO2 and soil temperature were significantly related (P < 0.002), with Q 10 values ranging between 1.75 and 2.53. Significant relationships (P < 0.05) were found between CH4 daily flux and WTD. Flood events resulted in enhanced CH4 emission (up to + 44.5 mg CH4-C m(-2) d(-1) in a swale) under warm soil conditions (> 22 degrees C), but the effect of flooding was less pronounced in early spring (emission < 1.06 mg CH4-C m(-2) d(-1)), probably due to low soil temperature. Although CH4 flux direction alternated at all sites, overall the croplands and the flood-affected riparian forest were CH4 sources, with annual emission averaging + 0.04 +/- 0.17 and + 0.92 +/- 1.6 kg CH4-C ha(-1), respectively. In the riparian forest, a topographic depression (< 8% of the total area) accounted for 78% of the annual CH4 emission, underscoring the significance of landscape heterogeneity on CH4 dynamics in riparian buffers. The nonflooded riparian grassland, however, was a net CH4 sink (-1.08 +/- 0.22 kg CH4-C ha(-1) yr(-1)), probably due to the presence of subsurface tile drains and a dredged/incised channel at that study site. Although these hydrological alterations may have contributed to improvement in the CH4 sink strength of the riparian grassland, this must be weighed against the water quality maintenance functions and other ecological services provided by riparian buffers. C1 [Jacinthe, P. A.; Liu, X.] Indiana Univ Purdue Univ, Dept Earth Sci, Indianapolis, IN 46202 USA. [Vidon, P.] SUNY ESF, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. [Fisher, K.] USDA ARS, Natl Soil Eros Lab, W Lafayette, IN 47907 USA. [Baker, M. E.] Univ Maryland Baltimore Cty, Dept Geog & Environm Syst, Baltimore, MD 21250 USA. RP Jacinthe, PA (reprint author), Indiana Univ Purdue Univ, Dept Earth Sci, Indianapolis, IN 46202 USA. EM pjacinth@iupui.edu FU USDA-NRI grant [2009-35112-05241] FX This work was supported by USDA-NRI grant 2009-35112-05241. The authors thank the landowners, Mr. Douglas Johnstone and Mr. Jeffrey Phares, for granting access to the study sites; Lauren Thomas and Lori Bebinger for field and lab assistance; and Ryan McAtee for construction of the static chambers. NR 41 TC 5 Z9 5 U1 4 U2 26 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2015 VL 44 IS 4 BP 1080 EP 1090 DI 10.2134/jeq2015.01.0014 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ8JC UT WOS:000360852600006 PM 26437089 ER PT J AU Ford, W King, K Williams, M Williams, J Fausey, N AF Ford, W. King, K. Williams, M. Williams, J. Fausey, N. TI Sensitivity Analysis of the Agricultural Policy/Environmental eXtender (APEX) for Phosphorus Loads in Tile-Drained Landscapes SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID WATER-QUALITY; UNITED-STATES; MODEL; MANAGEMENT; TRANSPORT; INDEXES; RUNOFF; SOIL; CALIBRATION; VALIDATION AB Numerical modeling is an economical and feasible approach for quantifying the effects of best management practices on dissolved reactive phosphorus (DRP) loadings from agricultural fields. However, tools that simulate both surface and subsurface DRP pathways are limited and have not been robustly evaluated in tile-drained landscapes. The objectives of this study were to test the ability of the Agricultural Policy/Environmental eXtender (APEX), a widely used field-scale model, to simulate surface and tile P loadings over management, hydrologic, biologic, tile, and soil gradients and to better understand the behavior of P delivery at the edge-of-field in tile-drained midwestern landscapes. To do this, a global, variance-based sensitivity analysis was performed, and model outputs were compared with measured P loads obtained from 14 surface and subsurface edge-of-field sites across central and northwestern Ohio. Results of the sensitivity analysis showed that response variables for DRP were highly sensitive to coupled interactions between presumed important parameters, suggesting nonlinearity of DRP delivery at the edge-of-field. Comparison of model results to edge-of-field data showcased the ability of APEX to simulate surface and subsurface runoff and the associated DRP loading at monthly to annual timescales; however, some high DRP concentrations and fluxes were not reflected in the model, suggesting the presence of preferential flow. Results from this study provide new insights into baseline tile DRP loadings that exceed thresholds for algal proliferation. Further, negative feedbacks between surface and subsurface DRP delivery suggest caution is needed when implementing DRP-based best management practices designed for a specific flow pathway. C1 [Ford, W.; King, K.; Williams, M.; Fausey, N.] USDA ARS, Columbus, OH 43210 USA. [Williams, J.] Texas A&M Univ, Blackland Res & Extens Ctr, Temple, TX 76502 USA. RP Ford, W (reprint author), USDA ARS, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM william.ford@ars.usda.gov FU 4R Research Fund; CEAP: Conservation Effects Assessment Project; Ohio Corn Growers (Ohio State University) [CIG: 69-3A75-12-231]; Heidelberg University [CIG: 69-3A75-13-216]; NRCS MRBI (Mississippi River Basin Initiative); [EPA: DW-12-92342501-0] FX The authors thank the land owners and operators at each of the study sites who provided access to their fields; Eric Fischer for analytical expertise; Jedediah Stinner, Katie Rumora, Marie Schrecengost, and Phil Levison for help in data collection and site maintenance; Dr. Elizabeth Dayton for providing soil data for many of the sites; and the two anonymous reviewers and the associate editor for insightful comments that significantly improved the quality of the manuscript. Partial funding for the data collection and analysis was provided by the 4R Research Fund, CEAP: Conservation Effects Assessment Project, EPA: DW-12-92342501-0, Ohio Corn Growers, CIG: 69-3A75-12-231 (Ohio State University), CIG: 69-3A75-13-216 (Heidelberg University), and the NRCS MRBI (Mississippi River Basin Initiative). NR 52 TC 2 Z9 2 U1 1 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2015 VL 44 IS 4 BP 1099 EP 1110 DI 10.2134/jeq2014.12.0527 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ8JC UT WOS:000360852600008 PM 26437091 ER PT J AU Muwamba, A Amatya, DM Ssegane, H Chescheir, GM Appelboom, T Tollner, EW Nettles, JE Youssef, MA Birgand, F Skaggs, RW Tian, S AF Muwamba, A. Amatya, D. M. Ssegane, H. Chescheir, G. M. Appelboom, T. Tollner, E. W. Nettles, J. E. Youssef, M. A. Birgand, F. Skaggs, R. W. Tian, S. TI Effects of Site Preparation for Pine Forest/Switchgrass Intercropping on Water Quality SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID THINNING LOBLOLLY-PINE; NORTH-CAROLINA; MANAGEMENT-PRACTICES; CONTROLLED DRAINAGE; COASTAL-PLAIN; SOUTH-CAROLINA; TAEDA L.; FOREST; SOIL; PLANTATION AB A study was initiated to investigate the sustainability effects of intercropping switchgrass (Panicum virgatum L.) in a loblolly pine (Pinus taeda L.) plantation. This forest-based biofuel system could possibly provide biomass from the perennial energy grass while maintaining the economics and environmental benefits of a forest managed for sawtimber. Operations necessary for successful switchgrass establishment and growth, such as site preparation, planting, fertilizing, mowing and baling, may affect hydrology and nutrient runoff. The objectives of this study were (i) to characterize the temporal effects of management on nutrient concentrations and loadings and (ii) to use pretreatment data to predict those treatment effects. The study watersheds (similar to 25 ha each) in the North Carolina Atlantic Coastal Plain were a pine/switchgrass intercropped site (D1), a midrotation thinned pine site with natural understory (D2), and a switchgrass-only site (D3). Rainfall, drainage, water table elevation, nitrogen (total Kjedahl N, NH4-N, and NO3-N), and phosphate were monitored for the 2007-2008 pretreatment and the 2009-2012 treatment periods. From 2010 to 2011 in site D1, the average NO3-N concentration effects decreased from 0.18 to -0.09 mg L-1, and loads effects decreased from 0.86 to 0.49 kg ha(-1). During the same period in site D3, the average NO3 -N concentration effects increased from 0.03 to 0.09 mg L-1, and loads effects increased from -0.26 to 1.24 kg ha(-1). This study shows the importance of considering water quality effects associated with intensive management operations required for switchgrass establishment or other novel forest-based biofuel systems. C1 [Muwamba, A.; Tollner, E. W.] Univ Georgia, Athens, GA 30602 USA. [Amatya, D. M.] USDA Forest Serv, Ctr Forested Wetlands Res, Cordesville, SC 29434 USA. [Ssegane, H.] Argonne Natl Lab, Argonne, IL 60439 USA. [Chescheir, G. M.; Appelboom, T.; Youssef, M. A.; Birgand, F.; Skaggs, R. W.; Tian, S.] N Carolina State Univ, Raleigh, NC 27695 USA. [Nettles, J. E.] Weyerhaeuser Co, Columbus, MS 39701 USA. RP Amatya, DM (reprint author), USDA Forest Serv, Ctr Forested Wetlands Res, Cordesville, SC 29434 USA. EM damatya@fs.fed.us FU Weyerhauser Company; Catch light Energy LLC (A Chevron/Weyerhaeuser Joint Venture); US Department of Energy FX The authors thank Weyerhauser Company, Catch light Energy LLC (A Chevron/Weyerhaeuser Joint Venture), and the US Department of Energy for logistical and financial support for this work; Tyson Cliff for helping in field data collection; and the peer and anonymous reviewers for their review and suggestions. NR 49 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2015 VL 44 IS 4 BP 1263 EP 1272 DI 10.2134/jeq2014.11.0505 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ8JC UT WOS:000360852600025 PM 26437108 ER PT J AU Taylor, JM Moore, MT Scott, JT AF Taylor, Jason M. Moore, Matthew T. Scott, J. Thad TI Contrasting Nutrient Mitigation and Denitrification Potential of Agricultural Drainage Environments with Different Emergent Aquatic Macrophytes SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CONVECTIVE GAS-FLOW; DISSIMILATORY NITRATE REDUCTION; MISSISSIPPI RIVER-BASIN; LOW-GRADE WEIRS; GULF-OF-MEXICO; CONSTRUCTED WETLAND; FRESH-WATER; NITROGEN-FIXATION; SEDIMENT; DITCHES AB Remediation of excess nitrogen (N) in agricultural runoff can be enhanced by establishing wetland vegetation, but the role of denitrification in N removal is not well understood in drainage ditches. We quantified differences in N retention during experimental runoff events followed by stagnant periods in mesocosms planted in three different vegetation treatments: unvegetated, cutgrass [Leersia oryzoides (L.) Sw.], and common cattail (Typha latifolia L.). We also quantified denitrification rates using membrane inlet mass spectrometry from intact cores extracted from each mesocosm treatment. All treatments retained 60% or more of NO3--N loads during the 6-h experimental runoff event, but mesocosms planted with cutgrass had significantly higher (68%) retention than the cattail (60%) or unvegetated (61%) treatments. After the runoff event, mesocosms planted in cattail reduced NO3--N concentrations by > 95% within 24 h and cutgrass achieved similar reductions within 48 h, whereas reductions in the unvegetated mesocosms were significantly less (65%). Cores from cutgrass mesocosms had significantly higher average denitrification rates (5.93 mg m(-2) h(-1)), accounting for as much as 56% of the immobilized NO3--N within 48 h, whereas denitrification rates were minimal in cores from the unvegetated (-0.19 mg m(-2) h(-1)) and cattail (0.2 mg m(-2) h(-1)) mesocosms. Our findings have implications for mitigating excess NO3--N in agricultural runoff. While vegetated treatments removed excess NO3--N from the water column at similar and significantly higher rates than unvegetated treatments, the high denitrification rates observed for cutgrass highlight the potential for permanent removal of excess N from agricultural runoff in vegetated ditches and wetlands. C1 [Taylor, Jason M.; Moore, Matthew T.] USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, Oxford, MS 38655 USA. [Scott, J. Thad] Univ Arkansas, Dept Crop & Soil Environm Sci, Fayetteville, AR 72701 USA. RP Taylor, JM (reprint author), USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, 598 McElroy Dr, Oxford, MS 38655 USA. EM jason.taylor@ars.usda.gov NR 65 TC 1 Z9 2 U1 3 U2 26 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2015 VL 44 IS 4 BP 1304 EP 1314 DI 10.2134/jeq2014.10.0448 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ8JC UT WOS:000360852600029 PM 26437112 ER PT J AU Kennedy, CD Buda, AR Kleinman, PJA DeMoranville, CJ AF Kennedy, Casey D. Buda, Anthony R. Kleinman, Peter J. A. DeMoranville, Carolyn J. TI Chemical and Isotopic Tracers Illustrate Pathways of Nitrogen Loss in Cranberry Floodwaters SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NUTRIENT; GROUNDWATER; WATER; MASSACHUSETTS; CATCHMENT; NITRATE; RUNOFF; INPUTS; O-18 AB Limited research exists on the sources of nitrogen (N) in cranberry floodwaters, which have been identified as a prominent cause of concern to watershed N loading in the cranberry-producing region of southeastern Massachusetts. In this study, we used naturally occurring chemical and isotopic tracers to infer the sources of N transported by harvest floodwaters. In 2012, the cranberry bed was a net source of total N (TN), exporting 0.8 kg N ha(-1) (primarily as organic N) to a nearby lake. Systematic increases in TN concentration were associated with increasing fractions of pre-event soil water and groundwater ("porewater") in discharge. Results showed that N concentrations in porewater generally derive from the natural mixing of soil water and perched groundwater within the cranberry bed but locally display a connection to deep groundwater where the underlying peat is absent. These findings illustrate the environmental significance of stored pools of porewater in cranberry beds and the ability to focus on moments of disproportionate N transfer to most efficiently curtail floodwater N losses (i.e., 58% of N export occurred in only 22% of floodwater discharge). C1 [Kennedy, Casey D.] USDA ARS, Pasture Syst & Watershed Management Res Unit, East Wareham, MA 02538 USA. [Kleinman, Peter J. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [DeMoranville, Carolyn J.] Univ Massachusetts, Cranberry Stn, East Wareham, MA 02538 USA. RP Kennedy, CD (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, East Wareham, MA 02538 USA. EM casey.kennedy@ars.usda.gov NR 35 TC 1 Z9 1 U1 0 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2015 VL 44 IS 4 BP 1326 EP 1332 DI 10.2134/jeq2014.12.0549 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ8JC UT WOS:000360852600032 PM 26437115 ER PT J AU Subbarao, KV Sundin, GW Klosterman, SJ AF Subbarao, Krishna V. Sundin, George W. Klosterman, Steven J. TI Focus Issue Articles on Emerging and Re-Emerging Plant Diseases SO PHYTOPATHOLOGY LA English DT Article AB The history of plant pathology is closely tied to plant diseases that have changed the course of human history. The Irish potato famine, caused by late blight of potato, resulted in the starvation and death of millions of people and one of the most influential human migrations in history. Other plant diseases have impacted quality of life in myriad ways. The advent of fungicides, clean seeds, host resistance, and the development of a plethora of other techniques, has diminished the impact of plant diseases. However, impressive advances in modes of rapid transport have not only increased global trade and human migration, but also augmented the risk for anthropogenic invasions of plant pathogens. As a consequence, and possibly aggravated by climate change, many historical and contemporary diseases are emerging as threats to modern agriculture and food security. These emerging diseases are not only important in global crop production, but also pose severe risks on a local level, especially on small farms in developing countries. This Focus Issue of Phytopathology contains a collection of peer-reviewed research articles, invited reviews and perspective articles on an assortment of emerging and re-emerging diseases caused by bacterial (3 papers), fungal (6 papers), oomycete (5 papers), and viral plant pathogens (3 papers). These diseases cover a range of crops including annual field crops and perennial tree crops, and vegetables, across five continents. The following are brief summaries of the papers appearing in this issue. C1 [Subbarao, Krishna V.] Univ Calif Davis, US Agr Res Stn, Salinas, CA 93905 USA. [Sundin, George W.] Michigan State Univ, E Lansing, MI 48824 USA. [Klosterman, Steven J.] USDA ARS, Salinas, CA 93905 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 NR 17 TC 2 Z9 2 U1 9 U2 45 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 852 EP 854 DI 10.1094/PHYTO-105-7-0001 PG 3 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100001 ER PT J AU Singh, RP Hodson, DP Jin, Y Lagudah, ES Ayliffe, MA Bhavani, S Rouse, MN Pretorius, ZA Szabo, LJ Huerta-Espino, J Basnet, BR Lan, CX Hovmoller, MS AF Singh, Ravi P. Hodson, David P. Jin, Yue Lagudah, Evans S. Ayliffe, Michael A. Bhavani, Sridhar Rouse, Matthew N. Pretorius, Zacharias A. Szabo, Les J. Huerta-Espino, Julio Basnet, Bhoja R. Lan, Caixia Hovmoller, Mogens S. TI Emergence and Spread of New Races of Wheat Stem Rust Fungus: Continued Threat to Food Security and Prospects of Genetic Control SO PHYTOPATHOLOGY LA English DT Article DE black rust ID F-SP TRITICI; ADULT-PLANT RESISTANCE; GRAMINIS F.SP TRITICI; NUCLEOTIDE-BINDING-SITE; PUCCINIA-GRAMINIS; POWDERY MILDEW; LEUCINE-RICH; SOUTH-AFRICA; AESTIVUM L.; BREAD WHEAT AB Race Ug99 (TTKSK) of Puccinia graminis f. sp. tritici, detected in Uganda in 1998, has been recognized as a serious threat to food security because it possesses combined virulence to a large number of resistance genes found in current widely grown wheat (Triticum aestivum) varieties and germplasm, leading to its potential for rapid spread and evolution. Since its initial detection, variants of the Ug99 lineage of stem rust have been discovered in Eastern and Southern African countries, Yemen, Iran, and Egypt. To date, eight races belonging to the Ug99 lineage are known. Increased pathogen monitoring activities have led to the identification of other races in Africa and Asia with additional virulence to commercially important resistance genes. This has led to localized but severe stem rust epidemics becoming common once again in East Africa due to the breakdown of race-specific resistance gene SrTmp, which was deployed recently in the 'Digalu' and 'Robin' varieties in Ethiopia and Kenya, respectively. Enhanced research in the last decade under the umbrella of the Borlaug Global Rust Initiative has identified various race-specific resistance genes that can be utilized, preferably in combinations, to develop resistant varieties. Research and development of improved wheat germplasm with complex adult plant resistance (APR) based on multiple slow-rusting genes has also progressed. Once only the Sr2 gene was known to confer slow rusting APR; now, four more genes-Sr55, Sr56, Sr57, and Sr58-have been characterized and additional quantitative trait loci identified. Cloning of some rust resistance genes opens new perspectives on rust control in the future through the development of multiple resistance gene cassettes. However, at present, disease-surveillance-based chemical control, large-scale deployment of new varieties with multiple race-specific genes or adequate levels of APR, and reducing the cultivation of susceptible varieties in rust hot-spot areas remains the best stem rust management strategy. C1 [Singh, Ravi P.; Basnet, Bhoja R.; Lan, Caixia] CIMMYT, Int Maize & Wheat Improvement Ctr, Mexico City 06600, DF, Mexico. [Hodson, David P.] CIMMYT, Addis Ababa, Ethiopia. [Jin, Yue; Rouse, Matthew N.; Szabo, Les J.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Lagudah, Evans S.; Ayliffe, Michael A.] Commonwealth Sci & Ind Res Org CSIRO Plant Ind, Agr Flagship, Canberra, ACT 2601, Australia. [Bhavani, Sridhar] CIMMYT, Nairobi 00621, Kenya. [Pretorius, Zacharias A.] Univ Orange Free State, ZA-9300 Bloemfontein, South Africa. [Huerta-Espino, Julio] Campo Expt Valle Mexico INIFAP, Chapingo 56230, Edo De Mexico, Mexico. [Hovmoller, Mogens S.] Aarhus Univ, Dept Agroecol, DK-4200 Slagelse, Denmark. RP Singh, RP (reprint author), CIMMYT, Int Maize & Wheat Improvement Ctr, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. EM R.Singh@cgiar.org RI Rouse, Matthew/G-5474-2011; Hovmoller, Mogens/E-8374-2016; OI bhavani, sridhar/0000-0002-4091-2608 FU CIMMYT; USDA-ARS; University of the Free State; CSIRO; INIFAP; Bill and Melinda Gates Foundation; DFID; USAID; ICAR-India; GRDC-Australia FX We salute the late Dr. Norman Borlaug for his vision and efforts to mobilize the global wheat community and donors to support international efforts to mitigate the threat from Ug99 and other P. graminis f. sp. tritici races. We thank our own institutions (CIMMYT, USDA-ARS, University of the Free State, CSIRO, and INIFAP) for their support, particularly the DRRW Project managed by Cornell University (supported by the Bill and Melinda Gates Foundation and DFID), USAID, ICAR-India, USDA-ARS, and GRDC-Australia, among others, for their financial support; the Kenyan Agricultural and Livestock Research Organization (KALRO), and Ethiopian Agricultural Research Institute (EIAR) for the provision of field and other facilities that have been crucial in generating some of the information presented in the article; and J. Mollins for technical editing of the article. NR 100 TC 23 Z9 23 U1 14 U2 46 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 872 EP 884 DI 10.1094/PHYTO-01-15-0030-FI PG 13 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100004 PM 26120730 ER PT J AU Kelly, HY Dufault, NS Walker, DR Isard, SA Schneider, RW Giesler, LJ Wright, DL Marois, JJ Hartman, GL AF Kelly, Heather Y. Dufault, Nicholas S. Walker, David R. Isard, Scott A. Schneider, Raymond W. Giesler, Loren J. Wright, David L. Marois, James J. Hartman, Glen L. TI From Select Agent to an Established Pathogen: The Response to Phakopsora pachyrhizi (Soybean Rust) in North America SO PHYTOPATHOLOGY LA English DT Article ID SOUTHEASTERN UNITED-STATES; GERMPLASM ACCESSIONS; CONFERS RESISTANCE; MODELING SYSTEM; CAUSAL AGENT; GLYCINE-MAX; 1ST REPORT; KUDZU; IDENTIFICATION; GENE AB The pathogen causing soybean rust, Phakopsora pachyrhizi, was first described in Japan in 1902. The disease was important in the Eastern Hemisphere for many decades before the fungus was reported in Hawaii in 1994, which was followed by reports from countries in Africa and South America. In 2004, P. pachyrhizi was confirmed in Louisiana, making it the first report in the continental United States. Based on yield losses from countries in Asia, Africa, and South America, it was clear that this pathogen could have a major economic impact on the yield of 30 million ha of soybean in the United States. The response by agencies within the United States Department of Agriculture, industry, soybean check-off boards, and universities was immediate and complex. The impacts of some of these activities are detailed in this review. The net result has been that the once dreaded disease, which caused substantial losses in other parts of the world, is now better understood and effectively managed in the United States. The disease continues to be monitored yearly for changes in spatial and temporal distribution so that soybean growers can continue to benefit by knowing where soybean rust is occurring during the growing season. C1 [Kelly, Heather Y.] Univ Tennessee, Dept Entomol & Plant Pathol, West Tennessee Res & Educ Ctr, Jackson, TN 38301 USA. [Dufault, Nicholas S.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Walker, David R.; Hartman, Glen L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Walker, David R.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Isard, Scott A.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. [Isard, Scott A.] Penn State Univ, Dept Meteorol, University Pk, PA 16802 USA. [Schneider, Raymond W.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Giesler, Loren J.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68182 USA. [Wright, David L.] Univ Florida, North Florida Res & Educ Ctr, Dept Agron, Quincy, FL 32351 USA. [Marois, James J.] Univ Florida, NFREC, Dept Plant Pathol, Quincy, FL 32351 USA. [Marois, James J.] Univ Florida, Dept Plant Pathol, NFREC, Gainesville, FL 32611 USA. RP Hartman, GL (reprint author), Univ Illinois, USDA ARS, Urbana, IL 61801 USA. EM glen.hartman@ars.usda.gov NR 112 TC 7 Z9 7 U1 1 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 905 EP 916 DI 10.1094/PHYTO-02-15-0054-FI PG 12 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100007 PM 25775102 ER PT J AU Olivera, P Newcomb, M Szabo, LJ Rouse, M Johnson, J Gale, S Luster, DG Hodson, D Cox, JA Burgin, L Hort, M Gilligan, CA Patpour, M Justesen, AF Hovmoller, MS Woldeab, G Hailu, E Hundie, B Tadesse, K Pumphrey, M Singh, RP Jin, Y AF Olivera, Pablo Newcomb, Maria Szabo, Les J. Rouse, Matthew Johnson, Jerry Gale, Samuel Luster, Douglas G. Hodson, David Cox, James A. Burgin, Laura Hort, Matt Gilligan, Christopher A. Patpour, Mehran Justesen, Annemarie F. Hovmoller, Mogens S. Woldeab, Getaneh Hailu, Endale Hundie, Bekele Tadesse, Kebede Pumphrey, Michael Singh, Ravi P. Jin, Yue TI Phenotypic and Genotypic Characterization of Race TKTTF of Puccinia graminis f. sp tritici that Caused a Wheat Stem Rust Epidemic in Southern Ethiopia in 2013-14 SO PHYTOPATHOLOGY LA English DT Article DE dispersal model; surveillance ID R-PACKAGE; VIRULENCE; POPULATIONS; TTKS; UG99 AB A severe stem rust epidemic occurred in southern Ethiopia during November 2013 to January 2014, with yield losses close to 100% on the most widely grown wheat cultivar, 'Digalu'. Sixty-four stem rust samples collected from the regions were analyzed. A meteorological model for airborne spore dispersal was used to identify which regions were most likely to have been infected from postulated sites of initial infection. Based on the analyses of 106 single-pustule isolates derived from these samples, four races of Puccinia graminis f. sp. tritici were identified: TKTTF, TTKSK, RRTTF, and JRCQC. Race TKTTF was found to be the primary cause of the epidemic in the southeastern zones of Bale and Arsi. Isolates of race TKTTF were first identified in samples collected in early October 2013 from West Arsi. It was the sole or predominant race in 31 samples collected from Bale and Arsi zones after the stem rust epidemic was established. Race TTKSK was recovered from 15 samples from Bale and Arsi zones at low frequencies. Genotyping indicated that isolates of race TKTTF belongs to a genetic lineage that is different from the Ug99 race group and is composed of two distinct genetic types. Results from evaluation of selected germplasm indicated that some cultivars and breeding lines resistant to the Ug99 race group are susceptible to race TKTTF. Appearance of race TKTTF and the ensuing epidemic underlines the continuing threats and challenges posed by stem rust not only in East Africa but also to wider-scale wheat production. C1 [Olivera, Pablo; Newcomb, Maria] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Szabo, Les J.; Rouse, Matthew; Johnson, Jerry; Gale, Samuel; Jin, Yue] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Luster, Douglas G.] USDA ARS, Foreign Disease Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Hodson, David] Int Maize & Wheat Improvement Ctr CIMMYT Ethiopia, Addis Ababa, Ethiopia. [Cox, James A.; Gilligan, Christopher A.] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England. [Burgin, Laura; Hort, Matt] UK Met Off, Exeter EX1 3PB, Devon, England. [Patpour, Mehran; Justesen, Annemarie F.; Hovmoller, Mogens S.] Aarhus Univ, Dept Agroecol, DK-4200 Slagelse, Denmark. [Woldeab, Getaneh; Hailu, Endale] Ethiopian Inst Agr Res, Ambo Agr Res Ctr, Addis Ababa, Ethiopia. [Hundie, Bekele] Ethiopian Inst Agr Res, Kulumsa Agr Res Ctr, Assela, Ethiopia. [Tadesse, Kebede; Pumphrey, Michael] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Singh, Ravi P.] CIMMYT, Mexico City 06600, DF, Mexico. RP Jin, Y (reprint author), Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM yuejin@umn.edu RI Rouse, Matthew/G-5474-2011; Hovmoller, Mogens/E-8374-2016; Justesen, Annemarie/E-9853-2016 FU Bill and Melinda Gates Foundation; United Kingdom Department for International Development; USDA-ARS FX This research was part of the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development. Additional support was received from the USDA-ARS National Plant Disease Recovery Plan. We thank L. Wanschura, S. Stoxen, and M. Carter for their technical assistance. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 27 TC 13 Z9 13 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 917 EP 928 DI 10.1094/PHYTO-11-14-0302-FI PG 12 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100008 PM 25775107 ER PT J AU Mahuku, G Lockhart, BE Wanjala, B Jones, MW Kimunye, JN Stewart, LR Cassone, BJ Sevgan, S Nyasani, JO Kusia, E Kumar, PL Niblett, CL Kiggundu, A Asea, G Pappu, HR Wangai, A Prasanna, BM Redinbaugh, MG AF Mahuku, George Lockhart, Benham E. Wanjala, Bramwel Jones, Mark W. Kimunye, Janet Njeri Stewart, Lucy R. Cassone, Bryan J. Sevgan, Subramanian Nyasani, Johnson O. Kusia, Elizabeth Kumar, P. Lava Niblett, C. L. Kiggundu, Andrew Asea, Godfrey Pappu, Hanu R. Wangai, Anne Prasanna, Boddupalli M. Redinbaugh, Margaret G. TI Maize Lethal Necrosis (MLN), an Emerging Threat to Maize-Based Food Security in Sub-Saharan Africa SO PHYTOPATHOLOGY LA English DT Article ID CHLOROTIC-MOTTLE-VIRUS; SUGARCANE-MOSAIC-VIRUS; LINKED IMMUNOSORBENT-ASSAY; SEED-TRANSMISSION; PLANT-VIRUSES; 1ST REPORT; MIXED INFECTIONS; COAT PROTEIN; SWEET CORN; HOST-RANGE AB In sub-Saharan Africa, maize is a staple food and key determinant of food security for smallholder farming communities. Pest and disease outbreaks are key constraints to maize productivity. In September 2011, a serious disease outbreak, later diagnosed as maize lethal necrosis (MLN), was reported on maize in Kenya. The disease has since been confirmed in Rwanda and the Democratic Republic of Congo, and similar symptoms have been reported in Tanzania, Uganda, South Sudan, and Ethiopia. In 2012, yield losses of up to 90% resulted in an estimated grain loss of 126,000 metric tons valued at $ 52 million in Kenya alone. In eastern Africa, MLN was found to result from coinfection of maize with Maize chlorotic mottle virus (MCMV) and Sugarcane mosaic virus (SCMV), although MCMV alone appears to cause significant crop losses. We summarize here the results of collaborative research undertaken to understand the biology and epidemiology of MLN in East Africa and to develop disease management strategies, including identification of MLN-tolerant maize germplasm. We discuss recent progress, identify major issues requiring further research, and discuss the possible next steps for effective management of MLN. C1 [Mahuku, George; Kimunye, Janet Njeri; Prasanna, Boddupalli M.] Int Maize & Wheat Improvement Ctr CIMMYT, Nairobi, Kenya. [Lockhart, Benham E.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Wanjala, Bramwel; Wangai, Anne] Kenya Agr & Livestock Res Org, Nairobi, Kenya. [Jones, Mark W.; Stewart, Lucy R.; Cassone, Bryan J.; Redinbaugh, Margaret G.] Ohio State Univ, USDA ARS, Corn Soybean & Wheat Qual Res, Wooster, OH 44691 USA. [Jones, Mark W.; Stewart, Lucy R.; Cassone, Bryan J.; Redinbaugh, Margaret G.] Ohio State Univ, Dept Plant Pathol, Wooster, OH 44691 USA. [Sevgan, Subramanian; Nyasani, Johnson O.; Kusia, Elizabeth] Int Ctr Insect Physiol & Ecol, Plant Hlth Div, Nairobi, Kenya. [Kumar, P. Lava] Int Inst Trop Agr, Ibadan, Nigeria. [Niblett, C. L.] Venganza Inc, St Augustine, FL 32080 USA. [Kiggundu, Andrew; Asea, Godfrey] Natl Agr Res Org, Entebbe, Uganda. [Pappu, Hanu R.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Redinbaugh, MG (reprint author), Ohio State Univ, USDA ARS, Corn Soybean & Wheat Qual Res, Wooster, OH 44691 USA. EM peg.redinbaugh@ars.usda.gov RI Redinbaugh, Margaret/A-3611-2013 FU Bill and Melinda Gates Foundation (BMGF) [24531, 23512]; BMGF; Syngenta Foundation for Sustainable Agriculture; CGIAR Research Program on Maize for implementing a project on MLN in eastern Africa; CRP MAIZE; CIMMYT; Kenya Agricultural Productivity and Agribusiness Project (KAPAP) Collaborative Research Projects; USAID's AfricaRISING project in Tanzania; Ministry of Agriculture-Kenya; KALRO FX We thank K. Willie (USDA-ARS), J. Vacha (USDA-ARS), and G. Raikhy (Washington State University) for providing expert technical assistance; One Acre Fund for allowing us to share results of MCMV and SCMV surveys done in collaboration with ARS; the Bill and Melinda Gates Foundation (BMGF) for support to M. G. Redinbaugh, C. L. Niblett, and B. E. Lockhart (contracts 24531 and 23512); BMGF and Syngenta Foundation for Sustainable Agriculture for providing financial support and CGIAR Research Program on Maize for implementing a project on MLN in eastern Africa; CRP MAIZE, CIMMYT, the Kenya Agricultural Productivity and Agribusiness Project (KAPAP) Collaborative Research Projects, USAID's AfricaRISING project in Tanzania, the Ministry of Agriculture-Kenya, and KALRO for their support; and One Acre Fund, USDA Foreign Agriculture Service, and USDA-ARS Office of International Research Programs for travel assistance for M. G. Redinbaugh and C. L. Niblett. NR 56 TC 6 Z9 7 U1 11 U2 31 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 956 EP 965 DI 10.1094/PHYTO-12-14-0367-FI PG 10 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100012 PM 25822185 ER PT J AU Fry, WE Birch, PRJ Judelson, HS Grunwald, NJ Danies, G Everts, KL Gevens, AJ Gugino, BK Johnson, DA Johnson, SB McGrath, MT Myers, KL Ristaino, JB Roberts, PD Secor, G Smart, CD AF Fry, W. E. Birch, P. R. J. Judelson, H. S. Gruenwald, N. J. Danies, G. Everts, K. L. Gevens, A. J. Gugino, B. K. Johnson, D. A. Johnson, S. B. McGrath, M. T. Myers, K. L. Ristaino, J. B. Roberts, P. D. Secor, G. Smart, C. D. TI Five Reasons to Consider Phytophthora infestans a Reemerging Pathogen SO PHYTOPATHOLOGY LA English DT Article ID POTATO FAMINE PATHOGEN; A2 MATING-TYPE; EASTERN UNITED-STATES; LATE-BLIGHT; DISEASE RESISTANCE; CLONAL LINEAGES; SEXUAL REPRODUCTION; RXLR EFFECTORS; CENTRAL MEXICO; MICROSATELLITE MARKERS AB Phytophthora infestans has been a named pathogen for well over 150 years and yet it continues to "emerge", with thousands of articles published each year on it and the late blight disease that it causes. This review explores five attributes of this oomycete pathogen that maintain this constant attention. First, the historical tragedy associated with this disease (Irish potato famine) causes many people to be fascinated with the pathogen. Current technology now enables investigators to answer some questions of historical significance. Second, the devastation caused by the pathogen continues to appear in surprising new locations or with surprising new intensity. Third, populations of P. infestans worldwide are in flux, with changes that have major implications to disease management. Fourth, the genomics revolution has enabled investigators to make tremendous progress in terms of understanding the molecular biology (especially the pathogenicity) of P. infestans. Fifth, there remain many compelling unanswered questions. C1 [Fry, W. E.; Danies, G.; Myers, K. L.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY 14850 USA. [Birch, P. R. J.] Univ Dundee, Div Plant Sci, James Hutton Inst, Dundee DD2 4DA, Scotland. [Judelson, H. S.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Gruenwald, N. J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Everts, K. L.] Univ Maryland, Dept Plant Pathol, Salisbury, MD 21801 USA. [Gevens, A. J.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Gugino, B. K.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, Coll Agr Sci, University Pk, PA 16802 USA. [Johnson, D. A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Johnson, S. B.] Univ Maine Cooperat Extens, Presque Isle, ME 04769 USA. [McGrath, M. T.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Long Isl Hort Res & Extens Ctr, Riverhead, NY 11901 USA. [Ristaino, J. B.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Roberts, P. D.] Univ Florida, Dept Plant Pathol, Southwest Florida Res & Educ Ctr, Immokalee, FL 34142 USA. [Secor, G.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Smart, C. D.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Barton Lab, NYSAES, Geneva, NY 14456 USA. RP Fry, WE (reprint author), Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, 334 Plant Sci Bldg, Ithaca, NY 14850 USA. EM wef1@cornell.edu OI Ristaino, Jean/0000-0002-9458-0514; Grunwald, Niklaus/0000-0003-1656-7602 FU Agriculture and Food Research Initiative Competitive Grants Program from United States Department of Agriculture [2011-68004-30154] FX The work reviewed here was supported by many institutions and funding agencies. Acquisition of the new data (Figures 5 through 7) was supported by our institutions and by the Agriculture and Food Research Initiative Competitive Grants Program (grant number 2011-68004-30154) from the United States Department of Agriculture. NR 140 TC 23 Z9 24 U1 13 U2 57 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 966 EP 981 DI 10.1094/PHYTO-01-15-0005-FI PG 16 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100013 PM 25760519 ER PT J AU Kamvar, ZN Larsen, MM Kanaskie, AM Hansen, EM Grunwald, NJ AF Kamvar, Z. N. Larsen, M. M. Kanaskie, A. M. Hansen, E. M. Gruenwald, N. J. TI Spatial and Temporal Analysis of Populations of the Sudden Oak Death Pathogen in Oregon Forests SO PHYTOPATHOLOGY LA English DT Article ID PHYTOPHTHORA-RAMORUM; MICROSATELLITE MARKERS; GENOTYPIC DIVERSITY; CALIFORNIA; LINEAGES; DISTANCES; EVOLUTION; MIGRATION; DYNAMICS; VIBURNUM AB Sudden oak death caused by the oomycete Phytophthora ramorum was first discovered in California toward the end of the 20th century and subsequently emerged on tanoak forests in Oregon before its first detection in 2001 by aerial surveys. The Oregon Department of Forestry has since monitored the epidemic and sampled symptomatic tanoak trees from 2001 to the present. Populations sampled over this period were genotyped using microsatellites and studied to infer the population genetic history. To date, only the NA1 clonal lineage is established in this region, although three lineages exist on the North American west coast. The original introduction into the Joe Hall area eventually spread to several regions: mostly north but also east and southwest. A new introduction into Hunter Creek appears to correspond to a second introduction not clustering with the early introduction. Our data are best explained by both introductions originating from nursery populations in California or Oregon and resulting from two distinct introduction events. Continued vigilance and eradication of nursery populations of P. ramorum are important to avoid further emergence and potential introduction of other clonal lineages. C1 [Kamvar, Z. N.; Hansen, E. M.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Larsen, M. M.; Gruenwald, N. J.] USDA ARS, Hort Crop Res Unit, Corvallis, OR 97333 USA. [Kanaskie, A. M.] Oregon Dept Forestry, Salem, OR USA. [Gruenwald, N. J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gruenwald, N. J.] Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crop Res Unit, Corvallis, OR 97333 USA. EM grunwaln@science.oregonstate.edu RI Kamvar, Zhian/C-5236-2017; OI Kamvar, Zhian/0000-0003-1458-7108; Grunwald, Niklaus/0000-0003-1656-7602 FU United States Department of Agriculture (USDA) Agricultural Research Service (ARS) [5358-22000-039-00D]; USDA Animal and Plant Health Inspection Service; USDA-ARS Floriculture Nursery Initiative; Oregon Department of Agriculture/Oregon Association of Nurseries (ODA-OAN); USDA Forest Service Forest Health Monitoring Program FX This work was supported, in part, by United States Department of Agriculture (USDA) Agricultural Research Service (ARS) grant 5358-22000-039-00D, USDA Animal and Plant Health Inspection Service, the USDA-ARS Floriculture Nursery Initiative, the Oregon Department of Agriculture/Oregon Association of Nurseries (ODA-OAN), and the USDA Forest Service Forest Health Monitoring Program. NR 49 TC 4 Z9 4 U1 3 U2 19 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 982 EP 989 DI 10.1094/PHYTO-12-14-0350-FI PG 8 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100014 PM 26068281 ER PT J AU Mathew, FM Alananbeh, KM Jordahl, JG Meyer, SM Castlebury, LA Gulya, TJ Markell, SG AF Mathew, Febina M. Alananbeh, Kholoud M. Jordahl, James G. Meyer, Scott M. Castlebury, Lisa A. Gulya, Thomas J. Markell, Samuel G. TI Phomopsis Stem Canker: A Reemerging Threat to Sunflower (Helianthus annuus) in the United States SO PHYTOPATHOLOGY LA English DT Article DE pathogenicity ID DIAPORTHE-HELIANTHI; MAXIMUM-LIKELIHOOD; MODEL; SUBSTITUTION; INCONGRUENCE; AUSTRALIA; PATHOGENS; BOOTSTRAP; REVEAL; RATES AB Phomopsis stem canker causes yield reductions on sunflower (Helianthus annuus L.) on several continents, including Australia, Europe, and North America. In the United States, Phomopsis stem canker incidence has increased 16-fold in the Northern Great Plains between 2001 and 2012. Although Diaporthe helianthi was assumed to be the sole causal agent in the United States, a newly described species, D. gulyae, was found to be the primary cause of Phomopsis stem canker in Australia. To determine the identity of Diaporthe spp. causing Phomopsis stem canker in the Northern Great Plains, 275 infected stems were collected between 2010 and 2012. Phylogenetic analyses of sequences of the ribosomal DNA internal transcribed spacer region, elongation factor subunit 1-alpha, and actin gene regions of representative isolates, in comparison with those of type specimens, confirmed two species (D. helianthi and D. gulyae) in the United States. Differences in aggressiveness between the two species were determined using the stem-wound method in the greenhouse; overall, D. helianthi and D. gulyae did not vary significantly (P <= 0.05) in their aggressiveness at 10 and 14 days after inoculation. These findings indicate that both Diaporthe spp. have emerged as sunflower pathogens in the United States, and have implications on the management of this disease. C1 [Mathew, Febina M.; Alananbeh, Kholoud M.; Jordahl, James G.; Meyer, Scott M.; Markell, Samuel G.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [Castlebury, Lisa A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Gulya, Thomas J.] USDA ARS Northern Crop Sci Lab, Fargo, ND 58108 USA. RP Markell, SG (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. EM samuel.markell@ndsu.edu FU National Sunflower Association (Mandan, ND) FX This research was supported by the National Sunflower Association (Mandan, ND). We thank P. Esker and J. LeBoldus for statistical advice; B. Nelson and T. Helms for providing helpful suggestions during revision of the manuscript; and E. Heitkamp, S. Choi, A. Streifel, C. Steffen, M. Ramsett, E. Crane, M. Gilley, N. Balbyshev, B. Lyslo, C. Taylor, and S. Choi for their technical assistance. NR 47 TC 4 Z9 4 U1 3 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 990 EP 997 DI 10.1094/PHYTO-11-14-0336-FI PG 8 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100015 PM 26121367 ER PT J AU Roy, A Hartung, JS Schneider, WL Shao, J Leon, G Melzer, MJ Beard, JJ Otero-Colina, G Bauchan, GR Ochoa, R Brlansky, RH AF Roy, Avijit Hartung, John S. Schneider, William L. Shao, Jonathan Leon, Guillermo Melzer, Michael J. Beard, Jennifer J. Otero-Colina, Gabriel Bauchan, Gary R. Ochoa, Ronald Brlansky, Ronald H. TI Role Bending: Complex Relationships Between Viruses, Hosts, and Vectors Related to Citrus Leprosis, an Emerging Disease SO PHYTOPATHOLOGY LA English DT Article DE Dichorhavirus; flat mite; virus replication ID BREVIPALPUS-PHOENICIS ACARI; COMPLETE NUCLEOTIDE-SEQUENCE; GENOMIC ORGANIZATION; CYTOPLASMIC-TYPE; B-OBOVATUS; CILV-C; TENUIPALPIDAE; MITES; PLANT; GENUS AB Citrus leprosis complex is an emerging disease in the Americas, associated with two unrelated taxa of viruses distributed in South, Central, and North America. The cytoplasmic viruses are Citrus leprosis virus C (CiLV-C), Citrus leprosis virus C2 (CiLV-C2), and Hibiscus green spot virus 2, and the nuclear viruses are Citrus leprosis virus N (CiLV-N) and Citrus necrotic spot virus. These viruses cause local lesion infections in all known hosts, with no natural systemic host identified to date. All leprosis viruses were believed to be transmitted by one species of mite, Brevipalpus phoenicis. However, mites collected from CiLV-C and CiLV-N infected citrus groves in Mexico were identified as B. yothersi and B. californicus sensu lato, respectively, and only B. yothersi was detected from CiLV-C2 and CiLV-N mixed infections in the Orinoco regions of Colombia. Phylogenetic analysis of the helicase, RNA-dependent RNA polymerase 2 domains and p24 gene amino acid sequences of cytoplasmic leprosis viruses showed a close relationship with recently deposited mosquito-borne negevirus sequences. Here, we present evidence that both cytoplasmic and nuclear viruses seem to replicate in viruliferous Brevipalpus species. The possible replication in the mite vector and the close relationship with mosquito borne negeviruses are consistent with the concept that members of the genus Cilevirus and Higrevirus originated in mites and citrus may play the role of mite virus vector. C1 [Roy, Avijit; Brlansky, Ronald H.] Univ Florida, IFAS, Dept Plant Pathol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Hartung, John S.; Shao, Jonathan] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. [Roy, Avijit; Schneider, William L.] USDA ARS, Foreign Disease Weed Sci Res Unit, Ft Detrick, MD USA. [Leon, Guillermo] CORPOICA, Ctr Invest La Libertad, Villavicencio, Colombia. [Melzer, Michael J.] Univ Hawaii, Plant & Environm Protect Sci, Honolulu, HI 96822 USA. [Beard, Jennifer J.] Queensland Museum, South Brisbane, Qld 4101, Australia. [Otero-Colina, Gabriel] Colegio Postgrad, Mexico City 56230, DF, Mexico. [Bauchan, Gary R.] USDA ARS, Electron & Confocal Microscopy Unit, Beltsville, MD USA. [Ochoa, Ronald] USDA ARS, Systemat Entomol Lab, Beltsville, MD USA. RP Brlansky, RH (reprint author), Univ Florida, IFAS, Dept Plant Pathol, Citrus Res & Educ Ctr, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. EM rhby@ufl.edu FU USDA-APHIS-PPQ; Citrus Research and Development Foundation [405] FX We thank D. Creel and N. Malikul (SEL-USDA), J. Mineiro, M. Sato, A. L. Matioli (Laboratorio de Acarologia, Centro Experimental do Instituto Biologico, Brazil), and J. E. Pena (Tropical Research and Education Center, University of Florida) for their continued support with Brevipalpus spp. specimen preparation, and data and reference collection. We thank A. Stone (FDWSRU, Ft. Detrick, MD) for his technical help and C. Pooley (E&CMU USDA-ARS) and C. Paul (EPCC, USDA-ARS), Beltsville, MD, for figure preparations. This research was financially supported by the USDA-APHIS-PPQ and the Citrus Research and Development Foundation Grant 405. NR 59 TC 3 Z9 3 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 1013 EP 1025 DI 10.1094/PHYTO-12-14-0375-FI PG 13 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100017 PM 25775106 ER PT J AU Sudarshana, MR Perry, KL Fuchs, MF AF Sudarshana, Mysore R. Perry, Keith L. Fuchs, Marc F. TI Grapevine Red Blotch-Associated Virus, an Emerging Threat to the Grapevine Industry SO PHYTOPATHOLOGY LA English DT Article ID ROLLING CIRCLE AMPLIFICATION; COMPLETE GENOME SEQUENCE; LEAF-CURL-VIRUS; DNA VIRUS; FAMILY GEMINIVIRIDAE; GENETIC DIVERSITY; POLYMERASE; DISEASE; PAPAYA; BEGOMOVIRUSES AB Grapevine red blotch-associated virus (GRBaV) is a newly identified virus of grapevines and a putative member of a new genus within the family Geminiviridae. This virus is associated with red blotch disease that was first reported in California in 2008. It affects the profitability of vineyards by substantially reducing fruit quality and ripening. In red-berried grapevine cultivars, foliar disease symptoms consist of red blotches early in the season that can expand and coalesce across most of the leaf blade later in the season. In white-berried grapevine cultivars, foliar disease symptoms are less conspicuous and generally involve irregular chlorotic areas that may become necrotic late in the season. Determining the GRBaV genome sequence yielded critical information for the design of primers for polymerase chain reaction-based diagnostics. To date, GRBaV has been reported in the major grape-growing areas in North America and two distinct phylogenetic clades have been described. Spread of GRBaV is suspected in certain vineyards but a vector of epidemiological significance has yet to be identified. Future research will need to focus on virus spread, the production of clean planting stocks, and the development of management options that are effective, economical, and environmentally friendly. C1 [Sudarshana, Mysore R.] Univ Calif Davis, Dept Plant Pathol, USDA ARS, Davis, CA 95616 USA. [Perry, Keith L.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Fuchs, Marc F.] Cornell Univ, New York State Agr Expt Stn, Sect Plant Pathol & Plant Microbe Biol, Sch Integrat Plant Sci, Geneva, NY 14456 USA. RP Fuchs, MF (reprint author), Cornell Univ, New York State Agr Expt Stn, Sect Plant Pathol & Plant Microbe Biol, Sch Integrat Plant Sci, Geneva, NY 14456 USA. EM mf13@cornell.edu FU American Vineyard Foundation; California Fruit Tree, Nut Tree, and Grapevine Improvement Advisory Board; USDA-NIFA-Specialty Crop Block Grant; New York Wine and Grape Foundation; USDA-ARS CRIS [5306-22000-014-00D] FX We thank our respective lab members J. van den Heuvel (Department of Horticulture, Cornell University), A. Walker and J. Wolpert (Department of Viticulture and Enology, University of California, Davis), and R. Smith (University of California ANR, Sonoma County, Santa Rosa); anonymous reviewers for their useful comments; and the numerous individuals who have contributed to this work, with our apologies to those left unmentioned. This research was supported, in part, by the American Vineyard Foundation; California Fruit Tree, Nut Tree, and Grapevine Improvement Advisory Board; USDA-NIFA-Specialty Crop Block Grant; New York Wine and Grape Foundation; and USDA-ARS CRIS 5306-22000-014-00D. NR 41 TC 10 Z9 11 U1 6 U2 31 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2015 VL 105 IS 7 BP 1026 EP 1032 DI 10.1094/PHYTO-12-14-0369-FI PG 7 WC Plant Sciences SC Plant Sciences GA CQ9IJ UT WOS:000360927100018 PM 25738551 ER PT J AU Thinakaran, J Pierson, E Kunta, M Munyaneza, JE Rush, CM Henne, DC AF Thinakaran, Jenita Pierson, Elizabeth Kunta, Madhurababu Munyaneza, Joseph E. Rush, Charlie M. Henne, Don C. TI Silver leaf Nightshade (Solanum elaeagnifolium), a Reservoir Host for 'Candidatus Liberibacter solanacearum', the Putative Causal Agent of Zebra Chip Disease of Potato SO PLANT DISEASE LA English DT Article ID COCKERELLI HEMIPTERA TRIOZIDAE; REAL-TIME PCR; 1ST REPORT; TOMATO; TRANSMISSION; ASSOCIATION; SULC AB Zebra chip disease of potato is caused by the bacterial pathogen 'Candidatus Liberibacter solanacearum' and is a growing concern for commercial potato production in several countries in North and Central America and New Zealand. 'Ca. L. solanacearum' is vectored by the potato psyllid Bactericera cockerelli, which transmits the pathogen to several cultivated and wild solanaceaous host plants. Silverleaf nightshade (SLN), Solanum elaeagnifolium, is a common weed in the Lower Rio Grande Valley of Texas and a host for both the potato psyllid and 'Ca. L. solanacearum'. SLN plants were successfully inoculated with 'Ca. L. solanacearum' under laboratory conditions. Retention studies demonstrated that 'Ca. L. solanacearum'-infected SLN planted in the field in January 2013, concurrent with commercial potato planting, retained the pathogen under field conditions throughout the year despite extensive dieback during summer. The presence of 'Ca. L. solanacearum' was confirmed in leaves, roots, and stolons of SLN plants collected the following year using polymerase chain reaction. Acquisition assays using B. cockerelli adults also revealed that SLN retained the pathogen. Transmission studies determined that B. cockerelli can acquire 'Ca. L. solanacearum' within a 2-week acquisition access period on 'Ca. L. solanacearum'-infected SLN and subsequently transmit the pathogen to potato. These results demonstrate that SLN plants can serve as a reservoir for 'Ca. L. solanacearum', providing a source of inoculum for B. cockerelli adults colonizing potato the next season. The presence of SLN plants all year round in the LRGV makes the weed an epidemiologically important host. These findings underscore the importance of eradicating or managing SLN plants growing in the vicinity of potato fields to prevent spread of 'Ca. L. solanacearum' and damage caused by zebra chip. C1 [Thinakaran, Jenita; Henne, Don C.] Texas A&M AgriLife Res, Weslaco, TX 78596 USA. [Pierson, Elizabeth] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Kunta, Madhurababu] Texas A&M Univ, Kingsville Citrus Ctr, Weslaco, TX 78599 USA. [Munyaneza, Joseph E.] USDA ARS, Wapato, WA 98951 USA. [Rush, Charlie M.] Texas A&M AgriLife Res & Extens Ctr, Amarillo, TX 79106 USA. RP Thinakaran, J (reprint author), USDA ARS, Wapato, WA 98951 USA. EM Jenita.Thinakaran@ars.usda.gov FU USDA RAMP [2009-51101-05892]; USDA SCRI [2009-51181-20178] FX We thank T. Salazar, A. Reyes Martinez, A. Pena, and E. Huerta for technical assistance; and J. C. Miller, J. G. Levy, A. Ravindran, C. Tamborindeguy, and D. Scheuring for their guidance and support. Funding was provided by USDA RAMP grant 2009-51101-05892 and USDA SCRI grant 2009-51181-20178. NR 36 TC 0 Z9 0 U1 2 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 910 EP 915 DI 10.1094/PDIS-12-14-1254-RE PG 6 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200002 ER PT J AU Bock, CH Hotchkiss, MW Cottrell, TE Wood, BW AF Bock, Clive H. Hotchkiss, Michael W. Cottrell, Ted E. Wood, Bruce W. TI The Effect of Sample Height on Spray Coverage in Mature Pecan Trees SO PLANT DISEASE LA English DT Article ID WATER-SENSITIVE PAPERS; CLADOSPORIUM-CARYIGENUM; APPLE-TREES; FUSICLADIUM-EFFUSUM; LEAF WETNESS; SCAB; QUALITY; PROPICONAZOLE; TEMPERATURE; DEPOSITION AB Pecan scab (caused by Fusicladium effusum) is the most damaging disease of pecan in the southeastern United States. Large air-blast sprayers for orchards are used to apply fungicide to control the disease but little quantitative information exists on the spray coverage achieved in the canopy of these trees. A series of experiments using water-sensitive spray cards to record spray coverage (percent area) at different heights and locations up to 15 m in the canopy of pecan trees showed a significantly greater percentage of card area covered at the lowest sample height when compared with the highest sample height. At the lowest height (5 m), spray coverage on individual cards ranged from 4.7 to 73.5% and, at the highest sample height (15 m), spray coverage ranged from 0.02 to 9.5%. In general, there was little significant difference in spray card coverage up to at least 10 m but, at 12.5 and 15 m, there was significantly less spray coverage compared with the coverage at 5 m. Regression analysis indicated a consistent linear relationship between sample height in the tree and the percent area covered. When spray cards were positioned at different heights without possible interference from pecan limbs and foliage, similar effects of sample height on spray coverage were noted. Wind speed measurements showed that air movement declined rapidly with distance from the sprayer fan. Whereas, at 2 m from the fan, wind speeds were approximately 26 m s(-1), by 10 m, speeds had declined to 2 to 4 m s(-1). At distances > 12 m, wind speed was approaching ambient air movement of about 1 to 3 m s(-1). Although aerial application resulted in numerically greater spray coverage at sample heights > 10 m, it was not significant even though a weak linear relationship (R-2 = 0.21 to 0.25) suggested an effect of height. Characterizing and understanding pesticide spray coverage in pecan will allow us to discern limits imposed by existing technology, and provide the basis for improving spray application methods (or tree management) for more efficacious disease control. C1 [Bock, Clive H.; Hotchkiss, Michael W.; Cottrell, Ted E.; Wood, Bruce W.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Bock, CH (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. EM clive.bock@ars.usda.gov FU Georgia Commodity Commission for Pecans FX We thank the Georgia Commodity Commission for Pecans in providing some financial support towards these studies, and W. Evans and T. Endalew for assistance with experiments and image analysis. NR 48 TC 1 Z9 1 U1 3 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 916 EP 925 DI 10.1094/PDIS-11-14-1154-RE PG 10 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200003 ER PT J AU Anco, DJ Poole, GH Gottwald, TR AF Anco, D. J. Poole, G. H. Gottwald, T. R. TI Postharvest Quarantine Treatments for Diaphorina citri on Infested Curry Leaves SO PLANT DISEASE LA English DT Article ID HOT-WATER IMMERSION; GLOBAL DISTRIBUTION; IONOSPHERE; VOLATILES; PH AB Studies were conducted to evaluate treatments that reduce survival and attachment of Diaphorina citri nymphs on infested curry leaves (Bergera koenigii). Decontamination of curry leaves infested with D. citri in relation to disinfectant (none or Pro-San), temperature (0, 40, and 50 degrees C), and treatment duration (0, 5, 10, and 20 min) was examined using a split-split plot design. Experiments were performed three times. Treatment duration did not significantly affect D. citri nymph survival or removal (P > 0.2). Temperature and disinfectant each significantly affected D. citri nymph survival and removal (P < 0.031). The interaction of temperature and disinfectant was significant with respect to nymph survival (P < 0.0001) but did not significantly affect removal (P = 0.4589). Tissue damage was significantly affected by temperature (P = 0.0056), duration (P = 0.0023), the interaction of temperature and duration (P = 0.0320), and the interaction of disinfectant, temperature, and duration (P = 0.0410). Of the treatments resulting in 100% D. citri nymph mortality on infested curry leaves, 40 degrees C for 5 min with Pro-San was accompanied with the least proportion of curly leaf tissue damage (0.14 greater than untreated control, P = 0.25). Results from these studies may be useful in formulation of future regulatory policies regarding trade of citrus foliage, especially those used as condiments. C1 [Anco, D. J.] N Carolina State Univ, Natl Sci Fdn, Ctr Integrated Pest Management, Raleigh, NC 27606 USA. [Anco, D. J.; Poole, G. H.; Gottwald, T. R.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Anco, DJ (reprint author), N Carolina State Univ, Natl Sci Fdn, Ctr Integrated Pest Management, Raleigh, NC 27606 USA. EM dan.anco@ars.usda.gov FU Institute of Food and Agricultural Sciences, University of Florida; USDA Animal and Plant Health Inspection Service PPQ CPHST; USDA-ARS FX Salaries and research support were provided by the Institute of Food and Agricultural Sciences, University of Florida, USDA Animal and Plant Health Inspection Service PPQ CPHST, and USDA-ARS. We thank F. Albano, G. Brock, J. Hodge, M. Ritenour, J. Smith, and L. Therrien for their contributions; D. Hall and A. Hill for their assistance in providing the initial D. citri population; and P. Nagy for posing a stimulating question. NR 52 TC 0 Z9 0 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 926 EP 932 DI 10.1094/PDIS-12-14-1271-RE PG 7 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200004 ER PT J AU Gurung, S Short, DPG Hu, XP Sandoya, GV Hayes, RJ Koike, ST Subbarao, KV AF Gurung, Suraj Short, Dylan P. G. Hu, Xiaoping Sandoya, German V. Hayes, Ryan J. Koike, Steven T. Subbarao, Krishna V. TI Host Range of Verticillium isaacii and Verticillium klebahnii from Artichoke, Spinach, and Lettuce SO PLANT DISEASE LA English DT Article ID LACTUCA-SATIVA L.; DAHLIAE; WILT; RESISTANCE; TRICORPUS; FREQUENCY AB Verticillium is a genus that includes major vascular wilt pathogens. Recently, multilocus phylogenetic analyses of the genus identified five new species, including Verticillium isaacii and V. klebahnii, both of which occur in agricultural soils in coastal California and have been isolated from asymptomatic and diseased spinach and lettuce plants. Little data are available regarding their pathogenicity and virulence on a broader range of crops important to the region. Four isolates each of V isaacii and V. klebahnii along with two reference isolates of V. dahliae races 1 and 2 were inoculated on eight crops (artichoke, cauliflower, eggplant, lettuce, pepper, tomato, spinach, and strawberry) in a greenhouse experiment. After 8 weeks, plants were assessed for disease severity to determine the relative host ranges of Verticillium isolates. Additionally, 13 lettuce lines resistant to race 1 and partially resistant to race 2 of V dahliae were screened against V isaacii and V. klebahnii to evaluate their responses. Three of four V isaacii and four of four V klebahnii isolates tested were nonpathogenic on all crops tested except those indicated below. One V isaacii isolate caused wilt on artichoke and 'Salinas' lettuce and most isolates of both species caused varying degrees of Verticillium wilt on strawberry. Lettuce lines resistant to V dahliae race 1 and partially resistant to V dahliae race 2 also exhibited resistance to all of the isolates of V. isaacii and V. klebahnii. Thus, at least some isolates in the populations of V isaacii and V. klebahnii have the potential to become significant pathogens of coastal California crops. However, resistance developed against V dahliae also offers resistance to the pathogenic isolates of both species, at least in lettuce. C1 [Gurung, Suraj; Short, Dylan P. G.; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Gurung, Suraj; Short, Dylan P. G.; Subbarao, Krishna V.] US Agr Res Stn, Salinas, CA 93905 USA. [Hu, Xiaoping] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. [Hu, Xiaoping] Northwest A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China. [Sandoya, German V.] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Sandoya, German V.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Hayes, Ryan J.] USDA ARS, Salinas, CA 93905 USA. [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA 93901 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU USDA-NIFA-SCRI [2010-51181-21069]; California Leafy Greens Research Board FX Funding for this study was provided by USDA-NIFA-SCRI grant number 2010-51181-21069 and the California Leafy Greens Research Board. We thank R. Marchebout for her excellent technical assistance and K. Maruthachalam for assistance with strawberry experiments. NR 24 TC 2 Z9 2 U1 2 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 933 EP 938 DI 10.1094/PDIS-12-14-1307-RE PG 6 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200005 ER PT J AU Zasada, IA Weiland, JE Han, Z Walters, TW Moore, P AF Zasada, Inga A. Weiland, Jerry E. Han, Z. Walters, T. W. Moore, P. TI Impact of Pratylenchus penetrans on Establishment of Red Raspberry SO PLANT DISEASE LA English DT Article ID PLANT-PARASITIC NEMATODES; 3 ONTARIO SOILS; BRITISH-COLUMBIA; ROOT-ROT; GROWTH; YIELD AB The plant-parasitic nematode, Pratylenchus penetrans, is a major constraint to red raspberry (Rubus idaeus) production. To determine the impact of P. penetrans on the establishment and productivity of eight raspberry cultivars, R. niveus, and R. leucoderans, plants were grown in fumigated and nonfumigated soil. Then, soil and root populations of P. penetrans and plant productivity (vigor, cane height, biomass, and yield) were monitored over 2 years. In a separate experiment, the role that soil type plays in mediating P. penetrans populations and raspberry establishment was investigated. At 6 and 12 months after planting, population densities of P. penetrans were lower in fumigated than in nonfumigated soil; this trend continued 18 months after planting. All cultivars and R. lettcodermis were found to be extremely susceptible to damage caused by P. penetrans. By the end of the experiment, yield was the most sensitive indicator of plant productivity among cultivars, with losses in nonfumigated soil ranging from 63 to 100% of those observed in fumigated soil. However, there was no difference in the productivity of R. niveus plants grown in nonfumigated versus fumigated soils. Results also indicated that the damage potential of P. penetrans was greater on sandy loam versus silt loam soils. The total plant biomass of 'Meeker' raspberry was 46% lower in a sandy loam soil containing P. penetrans compared with soil without P. penetrans; this trend was not observed consistently in a silt loam soil. Despite the fact that soil fumigation is increasingly more limited by regulations, this study shows the utility of fumigation in reducing P. penetrans populations for a sufficient period of time to ensure that newly planted raspberry seedlings can become successfully established. C1 [Zasada, Inga A.; Weiland, Jerry E.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Han, Z.; Walters, T. W.] Washington State Univ, Northwestern Washington Res & Extens Ctr, Mt Vernon, WA 98273 USA. [Moore, P.] Washington State Univ, Puyallup, WA 98371 USA. RP Zasada, IA (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. EM inga.zasada@ars.usda.gov FU United States Department of Agriculture NIFA-RAMP grant [2010-51101-21649] FX We thank J. Pinkerton, D. Kroese, A. Davis, B. Beck, and D. Wallace for technical assistance. This research was partially funded by the United States Department of Agriculture NIFA-RAMP grant 2010-51101-21649. NR 39 TC 4 Z9 4 U1 1 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 939 EP 946 DI 10.1094/PDIS-09-14-0980-RE PG 8 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200006 ER PT J AU Tewoldemedhin, YT Lamprecht, SC Mazzola, M AF Tewoldemedhin, Y. T. Lamprecht, S. C. Mazzola, M. TI Rhizoctonia Anastomosis Groups Associated with Diseased Rooibos Seedlings and the Potential of Compost as Soil Amendment for Disease Suppression SO PLANT DISEASE LA English DT Article ID SOUTH-AFRICA; PLANT-DISEASES; WESTERN-CAPE; DAMPING-OFF; SOLANI; PATHOGENICITY; PYTHIUM; SPP.; CROP; MANAGEMENT AB Rhizoctonia spp. associated with rooibos in the Western Cape province of South Africa were recovered during the 2008 season by planting seedlings in rhizosphere soils collected from 14 rooibos nurseries. In all, 75 Rhizoctonia isolates were obtained, of which 67 were multinucleate and 8 were binucleate Rhizoctonia spp. The identity of these isolates to anastomosis group (AG) was determined through sequence analysis of the ribosomal DNA internal transcribed spacer region. The collection of multinucleate isolates included representatives of AG-2-2 (67%), AG-4 HGI (14%), AG-11 (5%), and R. zeae (3%). Binucleate AGs included AG-Bo (4%) and AG-K (4%) and an unidentified binucleate Rhizoctonia (3%). Rhizoctonia solani AG-2-2 was the most widely distributed species of Rhizoctonia detected among the 11 nurseries sampled. All AGs recovered from rooibos have been previously reported on crop plants in South Africa, with the exception of R. zeae. However, this is the first study to classify the Rhizoctonia AGs recovered from rooibos. In glasshouse bioassays, the most virulent Rhizoctonia AGs on rooibos and lupin were AG-2-2, AG-4 HGI, and AG-11. Although plant damage was less than that observed for lupin and rooibos, oat was significantly affected by AG-2-2 and AG-4 HGI. Two composts sourced from independent suppliers were evaluated for disease suppression under glasshouse conditions. Compost amendment suppressed damping-off by most R. solani AGs, except for AG-4 HGI. Furthermore, within AG-2-2, suppression by compost was isolate specific. C1 [Tewoldemedhin, Y. T.; Lamprecht, S. C.] ARC Plant Protect Res Inst, ZA-7599 Stellenbosch, Western Cape, South Africa. [Mazzola, M.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Tewoldemedhin, YT (reprint author), ARC Plant Protect Res Inst, Private Bag X5017, ZA-7599 Stellenbosch, Western Cape, South Africa. EM tewoldemedhiny@arc.agric.za FU South African Rooibos Council; Agricultural Research Council FX We thank the South African Rooibos Council and Agricultural Research Council for financial support of the project; and A. Schoeman, T. Phasoana, A. Van den Heever, S. Williams, J. Lottering, and X. Xhwitha for technical assistance. NR 32 TC 0 Z9 0 U1 3 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 1020 EP 1025 DI 10.1094/PDIS-11-14-1211-RE PG 6 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200016 ER PT J AU Winger, BW AF Winger, B. W. TI First Report of White Fir Dwarf Mistletoe (Arceuthobium abietinum f. sp concoloris) on Engelmann Spruce (Picea engelmanii) From Oregon. SO PLANT DISEASE LA English DT News Item C1 USDA Forest Serv, Bend, OR 97701 USA. RP Winger, BW (reprint author), USDA Forest Serv, Bend, OR 97701 USA. NR 3 TC 0 Z9 0 U1 2 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 1041 EP 1042 PG 2 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200041 ER PT J AU Batuman, O Natwick, ET Wintermantel, WM Tian, T McCreight, JD Hladky, LL Gilbertson, RL AF Batuman, O. Natwick, E. T. Wintermantel, W. M. Tian, T. McCreight, J. D. Hladky, L. L. Gilbertson, R. L. TI First Report of an Ipomovirus Infecting Cucurbits in the Imperial Valley of California. SO PLANT DISEASE LA English DT News Item C1 [Batuman, O.; Gilbertson, R. L.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Natwick, E. T.] Univ Calif ANR Cooperat Extens Imperial Cty, Holtville, CA 92250 USA. [Wintermantel, W. M.; McCreight, J. D.; Hladky, L. L.] USDA ARS, Salinas, CA 93905 USA. [Tian, T.] Calif Dept Food & Agr, Sacramento, CA 95832 USA. RP Batuman, O (reprint author), Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. NR 4 TC 4 Z9 4 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2015 VL 99 IS 7 BP 1042 EP 1042 PG 1 WC Plant Sciences SC Plant Sciences GA CQ8NX UT WOS:000360866200043 ER PT J AU Xiao, X Kuang, X Sauer, TJ Heitman, JL Horton, R AF Xiao, X. Kuang, X. Sauer, T. J. Heitman, J. L. Horton, R. TI Bare Soil Carbon Dioxide Fluxes with Time and Depth Determined by High-Resolution Gradient-Based Measurements and Surface Chambers SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID GAS-DIFFUSION COEFFICIENT; MODELING DIFFUSION; POROUS SOLIDS; CO2; EFFLUX; FOREST; RESPIRATION; FIELD AB Soil CO2 production rates and fluxes vary with time and depth. The shallow near-surface soil layer is important for myriad soil processes, yet knowledge of dynamic CO2 concentrations and fluxes in this complex zone is limited. We used a concentration gradient method (CGM) to determine CO2 production and effluxes with depth in shallow layers of a bare soil. The CO2 concentration was continuously measured at 13 depths in the 0- to 200-mm soil layer. For an 11-d period, 2% of the soil CO2 was produced below a depth of 175 mm, 8% was produced in the 50- to 175-mm soil layer, and 90% was produced in the 0- to 50-mm soil layer. Soil CO2 concentration showed similar diurnal patterns with temperature in deeper soil layers and out-of-phase diurnal patterns in surface soil layers. Soil CO2 flux from most of the soil layers can be described by an exponential function of soil temperature, with temperature sensitivity (Q(10)) ranging from 1.40 to 2.00 (1.62 +/- 0.17). The temperature-normalized CO2 fluxes are related to soil water content with a positive linear relationship in surface soil layers and a negative relationship in deep soil layers. The CO2 fluxes from CGM and chamber methods had good agreement at multiple time scales, which showed that the CGM method was able to estimate near-surface soil CO2 fluxes and production. The contrasting patterns between surface and deep layers of soil CO2 concentration and fluxes suggest the necessity of intensive CO2 concentration measurements in the surface soil layer for accurate determination of soil-atmosphere CO2 flux when using the CGM. C1 [Xiao, X.; Kuang, X.; Heitman, J. L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Sauer, T. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Horton, R.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Heitman, JL (reprint author), N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. EM jlheitman@ncsu.edu FU National Science Foundation [0809656, 1215864]; Hatch Act, State of Iowa; State of North Carolina funds FX This work was supported by the National Science Foundation (Grants no. 0809656 and 1215864) and by the Hatch Act, State of Iowa, and State of North Carolina funds. NR 27 TC 1 Z9 1 U1 2 U2 13 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1073 EP 1083 DI 10.2136/sssaj2015.02.0079 PG 11 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300007 ER PT J AU Pachepsky, Y Park, Y AF Pachepsky, Yakov Park, Yongeun TI Saturated Hydraulic Conductivity of US Soils Grouped According to Textural Class and Bulk Density SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID WATER-RETENTION; PEDOTRANSFER FUNCTIONS; FIELD; INFILTRATION; SCALE AB The importance of saturated hydraulic conductivity (Ksat) as a soil hydraulic property led to the development of multiple pedotransfer functions for estimating it. One approach to estimating Ksat uses textural classes rather than specific textural fraction contents as a pedotransfer input. The objective of this work was to develop and evaluate a grouping-based pedotransfer procedure to estimate Ksat for sample sizes used in laboratory measurements. A search of publications and reports resulted in the collection of 1245 data sets with coupled data on Ksat, USDA textural class, and bulk density in the United States into a database called USKSAT. A separate database was assembled for the state of Florida that included 24,566 data sets. Data in each textural class were split into high and low bulk density groups using the splitting algorithm that created the most homogeneous groups. Sample diameters and lengths were <10 cm. Peaks of the semi-partial R-2 were well defined for loamy soils. The threshold bulk density separating high and low bulk density groups is 1.24 g cm(-3) for clay soils, about 1.33 g cm(-3) for loamy soils, and about 1.65 g cm(-3) for sandy soils. The high bulk density groups included a much broader range of Ksat values than the low bulk density groups for clays and loams but not sandy soils. Inspection of superimposed dependencies of Ksat on bulk density in the USKSAT database and in the Florida database showed their similarity. When geometric means were used as estimates of Ksat within groups, the accuracy was not high and yet was comparable with estimates obtained from far more detailed soil information using sophisticated machine learning methods. Estimating Ksat from textural class and bulk density may have the advantage of utility in data-poor environments and large-scale projects. C1 [Pachepsky, Yakov; Park, Yongeun] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Pachepsky, Y (reprint author), USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090; Park, Yongeun/0000-0002-1959-0843 NR 48 TC 5 Z9 5 U1 2 U2 18 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1094 EP 1100 DI 10.2136/sssaj2015.02.0067 PG 7 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300009 ER PT J AU Nottingham, AC Thompson, JA Turk, PJ Li, QC Connolly, SJ AF Nottingham, Adrienne C. Thompson, James A. Turk, Philip J. Li, Qiuchen Connolly, Stephanie J. TI Seasonal Dynamics of Surface Soil Bulk Density in a Forested Catchment SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER; NITROGEN; SYSTEMS; CARBON; DEPTH AB Bulk density is a commonly measured property during field investigations of soils. Accurate and reliable bulk density measurements are critical for assessing soil quality as well as for converting mass-based measurements to volume-based values. Our objective was to determine if there are significant seasonal changes to the measured bulk density of surface soil horizons in a forested ecosystem. The frame method was used to measure bulk density at monthly intervals for 12 mo at 10 locations within a forested catchment. The measured bulk density was variable with time for all plots, with the strongest seasonality shown in Oi horizons. Seasonal trends in Oe and A horizons were less pronounced. Particularly for O horizons, it appears to be critical to consider the time of year when collecting bulk density data. C1 [Nottingham, Adrienne C.; Thompson, James A.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Turk, Philip J.] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [Li, Qiuchen] W Virginia Univ, Dept Stat, Morgantown, WV 26506 USA. [Connolly, Stephanie J.] US Forest Serv, Monongahela Natl Forest, Elkins, WV 26241 USA. RP Thompson, JA (reprint author), W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. EM james.thompson@mail.wvu.edu FU USDA-NRCS FX Portions of this research were supported by the USDA-NRCS. Scientific Contribution no. 3245 from the West Virginia Agricultural and Forestry Experiment Station, Morgantown. We thank Josh Cook and Sara Jones for assistance in field data collection and Erin Jahnes for assistance with sample processing in the laboratory. NR 31 TC 0 Z9 0 U1 1 U2 7 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1163 EP 1168 DI 10.2136/sssaj2014.12.0491n PG 6 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300016 ER PT J AU Jin, VL Schmer, MR Wienhold, BJ Stewart, CE Varvel, GE Sindelar, AJ Follett, RF Mitchell, RB Vogel, KP AF Jin, Virginia L. Schmer, Marty R. Wienhold, Brian J. Stewart, Catherine E. Varvel, Gary E. Sindelar, Aaron J. Follett, Ronald F. Mitchell, Robert B. Vogel, Kenneth P. TI Twelve Years of Stover Removal Increases Soil Erosion Potential without Impacting Yield SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID CORN RESIDUE REMOVAL; NO-TILL CORN; CARBON SEQUESTRATION; ORGANIC-CARBON; PRODUCTION SYSTEMS; CROP RESPONSE; NITROGEN; QUALITY; MATTER; MANAGEMENT AB Corn (Zea mays L.) stover (non-grain aboveground biomass) in the US Corn Belt is used increasingly for livestock grazing and co-feed and for cellulosic bioenergy production. Continuous stover removal, however, could alter long-term agricultural productivity by affecting soil organic C (SOC) and soil physical properties, indicators of soil fertility and erosion potential. In this study, we showed that 12 consecutive yr of 55% stover removal did not affect mean grain yields at any N fertilizer rate (4.5, 6.3, and 6.0 Mg ha(-1) for 60, 120, and 180 kg N ha(-1) yr(-1), respectively) in a marginally productive, rainfed continuous corn system under no-till (NT). Although SOC increased in the top 30 cm of all soils since 1998 (0.54-0.79 Mg C ha(-1) yr(-1)), stover removal tended to limit SOC gains compared with no removal. Near-surface soils (0-5-cm depth) were more sensitive to stover removal and showed a 41% decrease in particulate organic matter stocks, smaller mean weight diameter of dry soil aggregates, and lower abundance of water-stable soil aggregates compared with soils with no stover removal. Increasing N fertilizer rate mitigated losses in total water-stable aggregates in near-surface soils related to stover removal. Collectively, however, our results indicated soil structure losses in surface soils due to lower C inputs. Despite no effect on crop yields and overall SOC gains with time using NT management, annually removing stover for 12 yr resulted in a higher risk of wind and water erosion at this NT continuous corn site in the western Corn Belt. C1 [Jin, Virginia L.; Schmer, Marty R.; Wienhold, Brian J.; Varvel, Gary E.; Sindelar, Aaron J.] Univ Nebraska, USDA ARS, Agroecosystem Management Res Unit, Lincoln, NE 68583 USA. [Stewart, Catherine E.; Follett, Ronald F.] ARS, USDA, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. [Mitchell, Robert B.; Vogel, Kenneth P.] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Jin, VL (reprint author), Univ Nebraska, USDA ARS, Agroecosystem Management Res Unit, 137 Keim Hall, Lincoln, NE 68583 USA. EM Virginia.Jin@ars.usda.gov OI Stewart, Catherine/0000-0003-1216-0450 FU USDA-ARS, Greenhouse Gas Reduction through Agricultural Carbon Enhancement Network (GRACEnet); Resilient Economic Agricultural Practices partnership (REAP); North Central Regional Sun Grant Center at South Dakota State University by the USDOE Office of Biomass Programs [DE-FC36-05GO85041] FX Project funding was provided by the USDA-ARS as part of the Greenhouse Gas Reduction through Agricultural Carbon Enhancement Network (GRACEnet) and Resilient Economic Agricultural Practices partnership (REAP; formally Renewable Energy Assessment Project). Additional funding was from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the USDOE Office of Biomass Programs under Award no. DE-FC36-05GO85041. We thank Steve Masterson, Patrick Callahan, Steve Swanson, Susan Siragusa, Elizabeth Pruessner, David Walla, Molly Hoffbauer, Tyler Goeschel, and Carla Ahlschwede for technical assistance in the field and laboratory. The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication does not imply recommendation or endorsement by the USDA. NR 75 TC 8 Z9 8 U1 7 U2 27 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1169 EP 1178 DI 10.2136/sssaj2015.02.0053 PG 10 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300017 ER PT J AU Wegner, BR Kumar, S Osborne, SL Schumacher, TE Vahyala, IE Eynard, A AF Wegner, Brianna R. Kumar, Sandeep Osborne, Shannon L. Schumacher, Thomas E. Vahyala, Ibrahim E. Eynard, Anna TI Soil Response to Corn Residue Removal and Cover Crops in Eastern South Dakota SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NO-TILL CORN; ORGANIC-CARBON; NITROGEN-FERTILIZATION; STOVER REMOVAL; WETTABILITY; QUALITY; IMPACT; DECOMPOSITION; CARBOHYDRATE; PRODUCTIVITY AB Excessive removal of crop residue has been shown to degrade soil organic carbon (SOC), and hence soil quality. Our objective was to assess the impacts of corn (Zea mays L.) residue removal and cover crops on various soil quality indicators. The experiment was conducted on a silty clay loam soil with and without a cover crop following three residue removal treatments. The low residue removal (LRR) treatment consisted of harvesting corn grain, leaving all other plant materials on the soil surface. Medium residue removal (MRR) consisted of harvesting grain, then chopping, windrowing and baling the remaining residue. The high residue removal (HRR) consisted of cutting the stalks 0.15 m from the ground and removing essentially all above-ground biomass. Crop residue removal significantly impacted measured soil properties including SOC, but cover cropping had minimal effects. The LRR treatment resulted in higher SOC concentrations and increased aggregate stability compared with other treatments. Residue removal significantly impacted the microbial activity as measured by hydrolysis of fluorescein diacetate (FDA). This study confirmed that HRR rates lead to SOC decomposition and adversely affect soil properties and soil quality. Soil conservation and emerging uses for crop residues must be balanced. Therefore, before making any decision to harvest crop residues, it is essential to have accumulated more C in the residue and supplemental cover crops than is needed to maintain equilibrium SOC levels. C1 [Wegner, Brianna R.; Kumar, Sandeep; Schumacher, Thomas E.; Eynard, Anna] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57006 USA. [Osborne, Shannon L.] ARS, USDA, NCARL, Brookings, SD 57006 USA. [Vahyala, Ibrahim E.] Modibbo Adama Univ Technol, Dept Soil Sci, Yola, Nigeria. RP Osborne, SL (reprint author), ARS, USDA, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM Shannon.Osborne@ars.usda.gov FU South Dakota Agriculture Experiment Station of South Dakota State University; North Cnetral Regional Project [NC-1178] FX We thank Kurt in soil smapling and the lab analysis. The authors acknowledge the partial funding support from South Dakota Agriculture Experiment Station of South Dakota State University, and in cooperation with the North Cnetral Regional Project NC-1178 (Soil Carbon Sequestration). NR 37 TC 2 Z9 2 U1 6 U2 22 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1179 EP 1187 DI 10.2136/sssaj2014.10.0399 PG 9 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300018 ER PT J AU Wuest, SB AF Wuest, Stewart B. TI Seasonal Variation in Soil Bulk Density, Organic Nitrogen, Available Phosphorus, and pH SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID BIOCHEMICAL ATTRIBUTES; CARBON; TILLAGE; PRAIRIE; TRENDS; MATTER AB Scientists are being called on to measure and predict the effects of soil management and climate change on organic matter and other soil properties. The estimates and predictions generated from current conditions and short-term experiments will only be accurate if our measurement techniques produce data that represent actual soil properties during the period of prediction. Perhaps the least studied aspect of soil sampling is the possibility that the timing of samples might introduce large non-random errors. This study sampled replicated plots every month for 39 mo. Even after removing the measurement variation caused by surface soil bulk density fluctuations, variations in organic N, available P, and pH were always > 10% of the mean. Much of the variation appeared to be temporally correlated in a seasonal cycle. In this experiment, with a 1-yr crop rotation, averaging 12 monthly samples allowed differences among soil treatments to be detected that were within 2 to 4% of the mean. When highly accurate estimates are desired, researchers need to consider combining multiple sample timings to overcome temporal variability. C1 USDA ARS, Adams, OR 97810 USA. RP Wuest, SB (reprint author), USDA ARS, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM stewart.wuest@ars.usda.gov NR 16 TC 0 Z9 0 U1 3 U2 20 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1188 EP 1197 DI 10.2136/sssaj2015.02.0066 PG 10 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300019 ER PT J AU Williams, JD Wuest, SB Robertson, DS AF Williams, John D. Wuest, Stewart B. Robertson, David S. TI Soil Water and Water-Use Efficiency in No-Tillage and Sweep Tillage Winter Wheat Production in Northeastern Oregon SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID INLAND PACIFIC-NORTHWEST; DRY SUMMER CLIMATE; CONSERVATION TILLAGE; MANAGEMENT INFLUENCE; CROPPING SYSTEMS; FALLOW; EVAPORATION; DYNAMICS; ROTATION AB The productivity of rainfed winter wheat (WW, Triticum aestivum L.) depends on the efficient capture and storage of precipitation. In the semi-arid Pacific Northwest (PNW), USA, soil water is managed through a 14-mo fallow period to establish wheat before winter and maximize growth potential the following spring. The effects of soil management on soil water storage were investigated on a Walla Walla silt loam soil (coarse-silty, mixed, superactive, mesic Typic Haploxerolls). The treatments were untilled chemical fallow (CF), versus a one-pass undercutter fallow (UF) in 2-yr WW-fallow rotations. These were compared with an annually cropped no-till WW (ANT). Measured from 2007 through 2014, CF had significantly more soil water than UF. Annually cropped no-till WW had less soil water than CF or UF for the duration of the research. These results were not reflected in precipitation capture efficiency. Annualized crop yield was significantly greater in ANT than in CF or UF, which were not significantly different. This resulted in water-use efficiency (WUE) being 30% greater in ANT than CF or UF. Unlike reports from drier zones of the PNW, it appears that CF and even annual wheat production may be ways to maximize WUE by wheat. C1 [Williams, John D.; Wuest, Stewart B.; Robertson, David S.] ARS, Columbia Plateau Conservat Res Ctr, USDA, Pendleton, OR 97801 USA. RP Williams, JD (reprint author), ARS, Columbia Plateau Conservat Res Ctr, USDA, POB 370, Pendleton, OR 97801 USA. EM john.williams@ars.usda.gov FU USDA-ARS national program Climate Change, Soils, and Emissions [212] FX We thank Bob Correa and Steve Umbarger (farm managers) and Tami Johlke (physical science technician) of the USDA-ARS Columbia Plateau Conservation Research Center, Pendleton, OR, for data acquisition and plot management. We also appreciate the substantial suggestions made by two anonymous reviewers and the associate editor, with which this effort was greatly improved. This research funded by USDA-ARS national program Climate Change, Soils, and Emissions (NP#212). NR 33 TC 4 Z9 4 U1 1 U2 7 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1206 EP 1212 DI 10.2136/sssaj2014.12.0494 PG 7 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300021 ER PT J AU Long, RP Bailey, SW Horsley, SB Hall, TJ Swistock, BR DeWalle, DR AF Long, Robert P. Bailey, Scott W. Horsley, Stephen B. Hall, Thomas J. Swistock, Bryan R. DeWalle, David R. TI Long-Term Effects of Forest Liming on Soil, Soil Leachate, and Foliage Chemistry in Northern Pennsylvania SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NORTHEASTERN UNITED-STATES; SUGAR MAPLE; ALLEGHENY PLATEAU; ACIDIC DEPOSITION; HARDWOOD FOREST; RED SPRUCE; NEW-YORK; NUTRITION; STRESS; GROWTH AB Sugar maple (Acer saccharum Marsh.) decline disease, decreased growth, and regeneration failure have been related to a low supply of Ca and Mg. There is increased interest in augmenting cation availability via liming, but there is little information on the amounts of lime required and the longevity of the lime treatment. A single application of 22.4 Mg ha(-1) of dolomitic limestone in 1985 at four forested sites in Potter County, PA has shown that soil, soil water, and sugar maple foliage chemistry are significantly altered by liming and the changes in soils and foliage persist as long as 21-yr post-treatment. By 2001, only 3 kg ha(-1) of lime remained undissolved while increases in exchangeable Ca and Mg, and pH continued through 2006 at depths up to 35 to 45 cm. Exchangeable Mn and Al concentrations were significantly reduced by lime application, though effects were slower to develop at deeper depths. Soil solution chemistry from 1996 was highly variable but reflected the changed soil nutrients. On limed plots, sugar maple foliage had elevated concentrations of Ca and Mg in 2006 samples, indicating the persistence of the lime treatment. Comparison of Ca/Al ratios in soil solution, soils, and foliage show inconsistencies from previous published risk thresholds. Species-specific thresholds of Ca and Mg availability in soil and foliage have better potential management application and should be more rigorously tested as stress indicators. C1 [Long, Robert P.; Horsley, Stephen B.] US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. [Bailey, Scott W.] US Forest Serv, USDA, No Res Stn, North Woodstock, NH 03262 USA. [Hall, Thomas J.] Penn Bur Forestry, Div Forest Pest Management, Harrisburg, PA 17105 USA. [Swistock, Bryan R.; DeWalle, David R.] Penn State Univ, University Pk, PA 16802 USA. RP Long, RP (reprint author), US Forest Serv, USDA, No Res Stn, 335 Natl Forge Rd, Irvine, PA 16329 USA. EM rlong@fs.fed.us OI Bailey, Scott/0000-0002-9160-156X FU Pennsylvania DCNR, Bureau of Forestry FX We gratefully acknowledge the long-term support of the Pennsylvania DCNR, Bureau of Forestry, for providing study sites, funding, and technical assistance for this research. We thank numerous people who helped with the study going back to its initiation by B. Towers, L. Auchmoody, P. Lilja, and others. Thanks also to those who helped with field sampling and data collection especially including: V. Brown, D. Eggen, T. Frontz, J. Hanson, W. Laubscher, B. Regester, D. Saf, J. Shaffer, A. Sidlinger, and E. Wiltsie. Statistical expertise and advice provided by J. Stanovick, mathematical statistician, is gratefully acknowledged. NR 41 TC 2 Z9 2 U1 2 U2 8 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2015 VL 79 IS 4 BP 1223 EP 1236 DI 10.2136/sssaj2014.11.0465 PG 14 WC Soil Science SC Agriculture GA CR0XZ UT WOS:000361048300023 ER PT J AU Solaiman, DKY Ashby, RD Zerkowski, JA Krishnama, A Vasanthan, N AF Solaiman, Daniel K. Y. Ashby, Richard D. Zerkowski, Jonathan A. Krishnama, Anusha Vasanthan, Nadarajah TI Control-release of antimicrobial sophorolipid employing different biopolymer matrices SO BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY LA English DT Article DE Algicidal; Antibacterial; Biopolymer film; Biosurfactant; Glycolipid ID PREPARING SOLID DISPERSIONS; HARMFUL ALGAL BLOOMS; POORLY SOLUBLE API; LISTERIA-MONOCYTOGENES; PSEUDOMONAS-OLEOVORANS; SALMONELLA-TYPHIMURIUM; COMPOSITE FILMS; FRESH BROCCOLI; MITIGATION; NANOCOMPOSITES AB Sophorolipid (SL) purified from fermentation broth of Candida bombicola grown on oleic acid and glucose substrates was embedded at 0%, 9%, 17%, and 29% (%-total weight of final product) in solvent-cast films of poly(L-lactic acid) (PLLA), poly(epsilon-caprolactone), and poly(hydroxybutyrate) (PHB). Growth-inhibition activity of the SL-biopolymers against Propionibacterium acnes, a causative agent of acne vulgaris skin condition, is dependent on the SL contents of the films; the degree of inhibition as determined from the width of the zone of inhibition in agar-plate assays follows the order of SL-PCL > SL-PLLA > SL-PHB. The release of SL from the films into aqueous medium after a 4-d shaking at 25 degrees C showed that SL-PLLA (30.1 +/- 1.7 wt% SL released) most readily released the embedded SL, followed by SL-PHB (11.4 + 4.3 wt%) and SL-PCL (4.3 +/- 1.4 wt%). Thermal properties as determined by differential scanning calorimetry showed that SL decreases the heat of fusion (Delta H) and the melting temperature (T-m) of the biopolymers, indicating for the first time its usefulness as a plasticizer to prevent crystallization. In summary, the study shows the feasibility of controlling the release of antimicrobial SL by varying the type of biopolymer used, with the added advantage of SL functioning as a plasticizer to improve the physical properties of the film in term of lower crystallinity. Future research could benefit the agricultural sector via new developments as varied as antimicrobial food packaging and algal bloom mitigation. (C) 2015 Published by Elsevier Ltd. C1 [Solaiman, Daniel K. Y.; Ashby, Richard D.; Zerkowski, Jonathan A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Krishnama, Anusha; Vasanthan, Nadarajah] Long Isl Univ, Dept Chem & Biochem, Brooklyn, NY USA. RP Solaiman, DKY (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Nadarajah.Vasanthan@liu.edu; dan.solaiman@ars.usda.gov NR 41 TC 1 Z9 1 U1 6 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1878-8181 J9 BIOCATAL AGRIC BIOTE JI Biocatal. Agric. Biotechnol. PD JUL PY 2015 VL 4 IS 3 BP 342 EP 348 DI 10.1016/j.bcab.2015.06.006 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CR1VE UT WOS:000361112400010 ER PT J AU Dulay, RMR Ray, K Hou, CT AF Dulay, Rich Milton R. Ray, Karen Hou, Ching T. TI Optimization of liquid culture conditions of Philippine wild edible mushrooms as potential source of bioactive lipids SO BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY LA English DT Article DE Basidiomycetes; Liquid cultivation; Bioactive lipids; Fatty acids; Triglycerides ID MYCELIAL GROWTH; SUBMERGED CULTURE; EXOPOLYSACCHARIDE AB With remarkable bioactivities and delightful taste, mushrooms have been a commercial nutraceutical around the world. Mushrooms are cultivated on solid materials. Here we report the successful cultivation of four Philippine edible mushrooms in liquid medium. This work highlights the optimal liquid culture conditions with reference to the nutritional and physical growth factors, and preliminarily elucidated their wide variety of lipids by thin-layer chromatography. Sabouraud dextrose broth (SDB) was the most suitable culture medium for all studied mushrooms. Maximum mycelial biomasses of Ganotiemia Mcidurn. Pleurotus cystidiosus, Volvariella volvacea and Schizophyllurn commune favorably produced in SDB at pH 7, 7, 6 and 8. respectively, when incubated at 28 degrees C and 30 degrees C as optimum temperatures. The mycelia biomass yields obtained in the present study 0.8 g/30 ml are superior to the reported biomass yields of other basidiomycetes such as Russula sp. and Pycnoponrs cinnabarinus at 0.3 g/50 ml (Shiite eI al., 20 05). Agitation did not improved mycelial growth of mushrooms except G. luciclum, which the biomass yield was peaked at 100 rpm (500 ml medium in 2.8 L) shake-flask culture. Cholesterol, triglycerides, free fatty acids, and polar lipids were detected in all the four mushrooms. The potential biological activities as well as molecular species of these lipid materials from the four basidiomycetes are currently under investigation. Published by Elsevier Ltd. C1 [Dulay, Rich Milton R.; Ray, Karen; Hou, Ching T.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Dulay, Rich Milton R.] Cent Luzon State Univ, Coll Arts & Sci, Dept Biol Sci, Ctr Trop Mushroom Res & Dev, Sci City Munoz 3120, Nueva Ecija, Philippines. RP Dulay, RMR (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM richmiltondulay@yahoo.com; ching.hou@ars.usda.gov FU Fulbright Philippine-American Educational Foundation (PAEF); U.S. Department of State's Bureau of Educational and Cultural Affairs; Council for International Exchange of Scholars (CIES); Fulbright-Philippine Agriculture Advanced Research Grant in the United States FX Financial support from the Fulbright Philippine-American Educational Foundation (PAEF), the U.S. Department of State's Bureau of Educational and Cultural Affairs and the Council for International Exchange of Scholars (CIES) is gratefully acknowledged, Mr. Rich Milton R. Dulay is a recipient of the Fulbright-Philippine Agriculture Advanced Research Grant in the United States (October 2014 April 2015). The remarkable technical supports of Kelly Utt and Trina Hartman of NCAUR, USDA are also highly appreciated. NR 39 TC 3 Z9 3 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1878-8181 J9 BIOCATAL AGRIC BIOTE JI Biocatal. Agric. Biotechnol. PD JUL PY 2015 VL 4 IS 3 BP 409 EP 415 DI 10.1016/j.bcab.2015.04.003 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CR1VE UT WOS:000361112400019 ER PT J AU Stevens, WB Evans, RG Iversen, WM Jabro, JD Sainju, UM Allen, BL AF Stevens, William B. Evans, Robert G. Iversen, William M. Jabro, Jalal D. Sainju, Upendra M. Allen, Brett L. TI Strip Tillage and High-Efficiency Irrigation Applied to a Sugarbeet-Barley Rotation SO AGRONOMY JOURNAL LA English DT Article ID FREQUENCY SPRINKLER IRRIGATION; CORN PRODUCTION; CROP PRODUCTION; WATER-USE; SYSTEM; YIELD; SOIL; LEPA; DEFICIT; ESTABLISHMENT AB Strip tillage (ST) and high-efficiency overhead irrigation methods reduce fuel and water inputs compared to conventional practices, but have not been extensively evaluated in sugarbeet (Beta vulgaris L.)-malt barley (Hordeum vulgare L.) cropping systems. A field study comparing conventional tillage (CT) and ST systems and two sprinkler irrigation methods (mid-elevation spray application, MESA; low-energy precision application, LEPA) was conducted near Sidney, MT, from 2004 to 2008. Strip tillage was performed (for sugarbeet only) using a single operation that left alternating 30-cm wide strips of tilled and untilled soil while fertilizer was simultaneously banded 10 cm below the seed row. Conventional tillage for sugarbeet consisted of six separate tillage operations following a broadcast application of fertilizer. Tillage preceding malt barley consisted of one pass each with a disk and a field cultivator following a broadcast application of fertilizer. Sugarbeet grown with ST yielded as well as when grown with CT. Irrigation method did not affect sugarbeet yield. Malt barley yield was not affected by irrigation method but was 5.4% lower following ST sugarbeet than when following CT sugarbeet. Tillage and fertilizer application costs for the sugarbeet phase of the 2-yr rotation were estimated to be approximately $141 ha(-1) less with ST than with CT representing reductions of 66% for the sugarbeet phase alone and 47% for the 2-yr cropping system. It was concluded that both ST and LEPA are applicable to irrigated sugarbeet-malt barley cropping systems and may provide substantial cost savings compared to conventional practices. C1 [Stevens, William B.; Evans, Robert G.; Iversen, William M.; Jabro, Jalal D.; Sainju, Upendra M.; Allen, Brett L.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Stevens, WB (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM bart.stevens@ars.usda.gov NR 51 TC 2 Z9 2 U1 5 U2 24 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1250 EP 1258 DI 10.2134/agronj14.0525 PG 9 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000005 ER PT J AU Meki, MN Kiniry, JR Youkhana, AH Crow, SE Ogoshi, RM Nakahata, MH Tirado-Corbala, R Anderson, RG Osorio, J Jeong, J AF Meki, Manyowa N. Kiniry, Jim R. Youkhana, Adel H. Crow, Susan E. Ogoshi, Richard M. Nakahata, Mae H. Tirado-Corbala, Rebecca Anderson, Ray G. Osorio, Javier Jeong, Jaehak TI Two-Year Growth Cycle Sugarcane Crop Parameter Attributes and Their Application in Modeling SO AGRONOMY JOURNAL LA English DT Article ID RADIATION-USE EFFICIENCY; HIGH INPUT CONDITIONS; INTERCEPTED SOLAR-RADIATION; BIOMASS ACCUMULATION; IRRIGATED SUGARCANE; YIELD ACCUMULATION; TROPICAL AUSTRALIA; LIGHT INTERCEPTION; DIVERSE SITES; ALMANAC MODEL AB The renewed interest in the use of sugarcane (Saccharin officinarum L.) for biofuel could provide a viable market for potential Hawaiian sugarcane feedstock producers. In Hawaii, sugarcane is grown as an irrigated 2-yr cycle crop. There is however little information on crop parameter attributes of 2-yr cycle sugarcane. This field study on Maui, Hawaii, analyzed the relationship between sugarcane biomass accumulation and specific crop parameters. Overall, the high dry biomass yield (80.20 Mg ha(-1)) was the result of a high leaf area index (LAI, 7.50) and radiation use efficiency (RUE, 2.06 g MJ(-1). The crop growth rate was highly correlated to LAI (R-2, 0.86), and a light extinction coefficient (k) of 0.53 was estimated. Stalk density was estimated at 18 stalks m(-2), with a maximum plant height of 3.6 m, and a rooting depth exceeding 2.0 m. When the crop parameters were incorporated into a biological model of Agricultural Land Management Alternatives with Numerical Assessment Criteria (ALMANAC) the model accurately simulated sugarcane yields across seven different soil types and multiple management scenarios of applied irrigation water, N and P fertilizer inputs and various planting and harvest dates. The mean simulation percent (%) errors ranged from -6.4% to 1.8%, while the calculated Fisher's paired t test of 1.41 with 39 degrees of freedom, showed no significant differences (P >= 0.05) between measured and simulated yields. The ALMANAC model should be useful as a decision support tool for evaluating sugarcane management alternatives that maximize yields while optimizing water, N and P inputs. C1 [Meki, Manyowa N.; Osorio, Javier; Jeong, Jaehak] Texas A&M AgriLife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Kiniry, Jim R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Youkhana, Adel H.; Crow, Susan E.] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Ogoshi, Richard M.] Univ Hawaii Manoa, Dept Trop Plants & Soil Sci, Honolulu, HI 96825 USA. [Nakahata, Mae H.] Hawaii Commercial & Sugar Co, Puunene, HI 96784 USA. [Tirado-Corbala, Rebecca] Univ Puerto Rico, Crops & Agroenvironm Sci, Mayaguez, PR USA. [Anderson, Ray G.] ARS, USDA, US Salin Lab, Contaminant Fate & Transport Unit,George E Brown, Riverside, CA 92507 USA. RP Meki, MN (reprint author), Texas A&M AgriLife Res, Blackland Res & Extens Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. EM nmeki@brc.tamus.edu OI Anderson, Ray/0000-0002-6202-5890 FU USDA-ARS, Grassland, Soil and Water Research Laboratory; Texas A&M AgriLife Research, Temple, TX [58-6206-1-053]; U.S. Navy, Office of Naval Research (ONR); ONR Grant [N00014-12-1-0496] FX Preparation of this manuscript is supported by the USDA-ARS, Grassland, Soil and Water Research Laboratory, and Texas A&M AgriLife Research, Temple, TX, through Specific Cooperative Agreement: 58-6206-1-053, and was funded by the U.S. Navy, Office of Naval Research (ONR). This work was also partly supported by ONR Grant N00014-12-1-0496 to the University of Hawaii. We thank Neil Abranyi, Jason Drogowski, Daniel Richardson, Meghan Pawlowski, and HC&S staff for field work. USDA is an equal opportunity provider and employer. NR 64 TC 1 Z9 1 U1 0 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1310 EP 1320 DI 10.2134/agronj14.0588 PG 11 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000011 ER PT J AU Shigaki, F Dell, CJ AF Shigaki, Francirose Dell, Curtis J. TI Comparison of Low-Cost Methods for Measuring Ammonia Volatilization SO AGRONOMY JOURNAL LA English DT Article ID SURFACE-APPLIED UREA; FOREST SOILS; NITROGEN; EMISSIONS; LOSSES; MANURE; FIELD; PASTURE; CHAMBER; AGRICULTURE AB Nitrogen fertilizer use to improve crop production is increasing worldwide, and subsequent N losses via NH3 emissions generate undesirable economic and environmental consequences. Thus, low cost and practical methods to quantify NH3 emissions are essential for the development of management practices that minimize environmental impacts. The objective of this study was to compare different methods to quantify NH3 loss following urea application to a grass field and indoor soil boxes. The methods tested were: semi-open chamber (SOC), open-collector (OC), closed chamber (CC), and a recirculating chamber (RC). The SOC and OC were shown to be equally efficient. Cumulative recoveries through 216 h for the outdoor study were 9.5 and 8.5 kg NH3-N ha(-1) for OC and SOC (respectively), corresponding to 8 and 7% of the applied N. The closed-chamber recovered only 3.6% of applied N. For the indoor study, higher recovery rates were observed with all methods. Cumulative losses measured through 216 h did not differ between SOC and OC (24.4 and 24.9 kg NH3-N, respectively) and represented about 20% of N applied. However RC and CC recovered 10 to 12% of the applied N (14.8 and 12 kg NH3-N ha(-1), respectively), which was significantly less than amounts recovered with OC and SOC. Results from this study showed that SOCs and OCs are simple, practical, and low cost tools for quantifying NH3 emissions either for indoor and outdoor studies compared with closed and recirculating chambers which presented disadvantages such as low NH3 recovery and higher costs. C1 [Shigaki, Francirose] Univ Fed Maranhao, Ctr Agrarian Sci, BR-65500000 Chapadinha, MA, Brazil. [Dell, Curtis J.] USDA ARS, Pasture Syst & Watershed Management Unit, University Pk, PA 16802 USA. RP Shigaki, F (reprint author), Univ Fed Maranhao, Ctr Agrarian Sci, Rd BR 222 Km 04, BR-65500000 Chapadinha, MA, Brazil. EM francirose@yahoo.com.br FU CAPES-Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior FX The authors would like to thank the staff of the USDA-ARS, Pasture Systems and Watershed Management Research Unit, University Park, PA, for their valuable contribution to this study: Sarah Fishel, Bart Moyer, Emily Bachner, and Curtis Kennedy who helped conducting the field and indoor studies; and Lou Saporito for the support on the statistical analysis; and CAPES-Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior for funding Francirose Shigaki to conduct this research. NR 49 TC 2 Z9 2 U1 6 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1392 EP 1400 DI 10.2134/agronj14.0431 PG 9 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000019 ER PT J AU Trippe, KM Griffith, SM Banowetz, GM Whitaker, GW AF Trippe, Kristin M. Griffith, Stephen M. Banowetz, Gary M. Whitaker, Gerald W. TI Changes in Soil Chemistry following Wood and Grass Biochar Amendments to an Acidic Agricultural Production Soil SO AGRONOMY JOURNAL LA English DT Article ID PACIFIC-NORTHWEST; CHEMICAL-CHARACTERIZATION; LIMING AGENT; ASH; GASIFICATION; STRAW; METAANALYSIS; CARBON; CHARS; SEED AB The ability of biochars that are produced by biomass gasification to remediate acidic production soils is generally not as well known in comparison to biochars resulting from pyrolysis. A recent characterization of biochar produced from gasification of Kentucky bluegrass (Poa pratensis L.) seed screenings (KB) suggested that KB biochar might have utility in remediation of acidic agricultural production soils where aluminum uptake limits wheat (Triticum aestivum L.) growth. A replicated greenhouse pot study was conducted in which single plants of wheat were grown for 74 d in a Freeman (fine-silty, mixed, superactive, mesic Aquandic Palexeralf) or Bernhill (fine-loamy, isotic, mesic Vitrandic Haploxeralf) soil amended with biochars produced by gasification of either KB seed screenings or chipped conifer tree tops. Addition of either biochar to the soils immobilized soil-Al. Selective binding characteristics of the biochars for some ions, heavy metals, organics, and the capacity to alter acid soil pH suggest a potential utility in acid soil remediation. C1 [Trippe, Kristin M.; Griffith, Stephen M.; Banowetz, Gary M.; Whitaker, Gerald W.] USDA ARS, Natl Forage Seed & Prod Res Ctr, Corvallis, OR 97331 USA. RP Trippe, KM (reprint author), USDA ARS, Natl Forage Seed & Prod Res Ctr, Corvallis, OR 97331 USA. EM kristin.trippe@ars.usda.gov NR 29 TC 3 Z9 3 U1 2 U2 26 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1440 EP 1446 DI 10.2134/agronj14.0593 PG 7 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000023 ER PT J AU Jabro, JD Iversen, WM Stevens, WB Evans, RG Mikha, MM Allen, BL AF Jabro, Jalal D. Iversen, William M. Stevens, William Bart Evans, Robert G. Mikha, Maysoon M. Allen, Brett L. TI Effect of Three Tillage Depths on Sugarbeet Response and Soil Penetrability Resistance SO AGRONOMY JOURNAL LA English DT Article ID PHYSICAL-PROPERTIES; DEEP-TILLAGE; CLAY LOAM; PENETRATION RESISTANCE; HYDRAULIC-PROPERTIES; WESTERN-AUSTRALIA; CORN RESPONSE; STRIP TILLAGE; BULK-DENSITY; ROOT-GROWTH AB Tillage can alter soil properties and affect crop yield and quality. A 4-yr study was conducted on a Lihen sandy loam soil loam (sandy, mixed, frigid Entic Haplustoll) to evaluate the effect of tillage depth on sugarbeet (Beta vulgaris L.) root yield, root quality, and soil penetration resistance (PR). Tillage treatments consisted of no-tillage (NT), shallow tillage (ST), and deep tillage (DT). Soil PR was measured with a penetrometer in 2.5-cm increments to a 40-cm depth at three locations within each plot. Roots were hand harvested from each plot and each sample consisted of roots of two adjacent rows. Sugarbeet root yield and adjusted sucrose yield were not significantly affected by the depth of tillage in 2008, 2009, and 2011. In 2010, root yield was significantly greater (16.5%) in DT than in NT. The average sugarbeet yields across 4 yr were 58.77, 60.30, and 63.03 Mg ha(-1) for NT, ST, and DT, respectively. Root yield was lower in 2011 than other 3 yr due to cold and wet weather conditions in the spring. Soil PR values were significantly lower in DT than in ST and NT from 5-to 30-cm depth. However, significant differences were found between ST and NT at the 5-to 7.5-cm depth. Overall, DT enhanced soil physical environment but on average across 4 yr had no statistically significant effect on sugarbeet root yield and quality compared to both ST and NT practices, though a trend was noted that as tillage depth increased root yield increased. C1 [Jabro, Jalal D.; Iversen, William M.; Stevens, William Bart; Evans, Robert G.; Allen, Brett L.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Mikha, Maysoon M.] Cent Plains Resources Management Res, Akron, CO 80720 USA. RP Jabro, JD (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM jay.jabro@ars.usda.gov NR 31 TC 2 Z9 2 U1 4 U2 9 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1481 EP 1488 DI 10.2134/agronj14.0561 PG 8 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000028 ER PT J AU Halvorson, AD Stewart, CE AF Halvorson, Ardell D. Stewart, Catherine E. TI Stover Removal Affects No-Till Irrigated Corn Yields, Soil Carbon, and Nitrogen SO AGRONOMY JOURNAL LA English DT Article ID CROP RESIDUE REMOVAL; OXIDE EMISSIONS; CONVENTIONAL-TILL; ORGANIC-CARBON; MANAGEMENT; BIOENERGY; GRAIN; FERTILIZATION; PRODUCTIVITY; SWITCHGRASS AB Corn (Zea mays L.) stover removal can increase yields under no-till (NT) in climates where cold spring soil temperatures delay emergence and plant growth. The study objective was to evaluate partial stover removal (PR) effects on irrigated NT corn grain and stover yields, N uptake, and changes in soil organic carbon (SOC) and total soil nitrogen (TSN) compared to full stover retained (FR) under three N treatments on a clay loam soil. Stover removal (average 66%) increased early spring soil temperatures and enhanced early plant development compared to FR. Grain and stover yields increased with increasing N rate, as did plant N and C uptake, but varied with stover treatment. Averaged over N rate, grain yields were greater with PR than FR, but stover yields were lower with PR than FR. The C/N ratio of stover declined with increasing N rate. Nitrogen fertilization did not significantly influence SOC and TSN stocks aft er 7 yr. Averaged over N rates, PR removal decreased SOC 2.4 Mg SOC ha(-1), but FR increased SOC 6.8 Mg SOC ha(-1) in 0- to 30-cm soil depth. TSN stocks increased significantly with FR (1176 kg N ha(-1)) but not with PR (70 kg N ha(-1)). These results suggest that continued, long-term residue removal will negate any initial yield benefits from more rapid early spring plant development. Partial stover removal as a cellulosic feedstock at the levels we used for ethanol production would negatively impact soil quality under irrigated, NT corn production in Colorado. C1 [Halvorson, Ardell D.; Stewart, Catherine E.] USDA ARS, Ft Collins, CO 80526 USA. RP Halvorson, AD (reprint author), USDA ARS, 2150 Ctr Ave,Bldg D,Ste 100, Ft Collins, CO 80526 USA. EM halvorson@ars.usda.gov OI Stewart, Catherine/0000-0003-1216-0450 FU Agricultural Research Service under the ARS GRACEnet Project FX The authors thank C. Reule, B. Floyd, R. D'Adamo, T. Delorean, and P. Norris for their assistance and analytical support in collecting, processing, and analyzing the soil and plant samples and data reported herein and the ARDEC Staff for help with plot maintenance. This publication is based on work supported by the Agricultural Research Service under the ARS GRACEnet Project. NR 40 TC 5 Z9 5 U1 2 U2 17 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1504 EP 1512 DI 10.2134/agronj15.0074 PG 9 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000030 ER PT J AU Amaral, LR Molin, JP Schepers, JS AF Amaral, Lucas R. Molin, Jose P. Schepers, James S. TI Algorithm for Variable-Rate Nitrogen Application in Sugarcane Based on Active Crop Canopy Sensor SO AGRONOMY JOURNAL LA English DT Article ID INFRARED REFLECTANCE SPECTROSCOPY; IN-SEASON; OPTIMIZATION ALGORITHM; RESPONSE INDEX; USE EFFICIENCY; CORN; FERTILIZATION; YIELD; SOIL; SYSTEM AB Nitrogen fertilization is challenging for sugarcane (Saccharum spp.) producers due to its complex interaction with the crop and soil. Thus, the main goal of this study was to develop a feasible approach to guide variable-rate N application in sugarcane based on canopy sensor readings. This study was conducted for 5 yr. Several plot and strip N-rate experiments were conducted under a wide range of crop conditions in Brazil and evaluated with the Crop Circle active canopy sensor (Holland Scientific Inc.). Because of variability in crop density and growth development within sugarcane fields, the use of an N-rich reference area to estimate the crop response to N application was compromised. Biomass was the main crop parameter influencing canopy sensor readings, allowing yield estimation because biomass typically results in stalk yield. Thus, canopy sensor readings can efficiently predict relative sugarcane yield when working with data that are normalized to the mean for the field. Hence, an algorithm that takes into account this relationship was established. The concept of this algorithm is to apply higher N fertilization rates where the sugarcane yield potential is higher. Such an approach was determined to be useful to guide N application in sugarcane fields. Nevertheless, field validation is needed to confirm this N management strategy. Besides, more information about sugarcane biomass variability within fields may be required to increase algorithm efficiency. C1 [Amaral, Lucas R.; Molin, Jose P.] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biosyst Engn, Piracicaba, SP, Brazil. [Schepers, James S.] USDA ARS, Lincoln, NE 68588 USA. [Schepers, James S.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68588 USA. RP Amaral, LR (reprint author), Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Biosyst Engn, Piracicaba, SP, Brazil. EM lucasamaral@agronomo.eng.br OI Amaral, Lucas/0000-0001-8071-4449 FU Research and Projects Financing (FINEP) from the Ministry of Science and Technology through the PROSENSAP project; National Council for Scientific and Technological Development (CNPq); Sao Paulo Research Foundation (FAPESP) [2009/03372-0, 2011/08882-7, 2012/03897-9] FX We acknowledge the Research and Projects Financing (FINEP) from the Ministry of Science and Technology through the PROSENSAP project and the National Council for Scientific and Technological Development (CNPq) for their financial support; Sao Paulo Research Foundation (FAPESP) for providing a graduate scholarship to L.R. Amaral (Projects no. 2009/03372-0 and 2011/08882-7) and for financing his period working at the University of Nebraska (Project no. 2012/03897-9); the University of Nebraska at Lincoln for receiving L.R. Amaral; Sao Martinho's Mill team, Sugarcane Technology Center (CTC), and Maquinas Agricolas Jacto SA for the partnership and assistance with the experiments; and Dr. Heitor Cantarella, from the Agronomic Institute of Campinas (IAC) for permitting the use of some of his experiments. NR 48 TC 1 Z9 1 U1 3 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1513 EP 1523 DI 10.2134/agronj14.0494 PG 11 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000031 ER PT J AU Morton, LW AF Morton, Lois Wright TI Achieving Water Security in Agriculture: The Human Factor SO AGRONOMY JOURNAL LA English DT Article ID MANAGEMENT AB It is widely recognized that achieving water security will take substantive investments in hydrology, engineering, soil science, agronomy, and a wide variety of physical and natural sciences and technologies. Less understood is the human aspect, the social science of beliefs, values, human perceptions and decision-making, social relationships, and social organization that intentionally and unintentionally construct, destroy, and reconstruct the water and land resources to which society is intimately linked. Addressing the complex issues of water security will require humans to acknowledge the threats to security and a willingness to give priority to assuring water quality, water availability, and water access to meet the needs of a growing world population and their economic engines. Soil-water-vegetation-climate-human relationships are central to maintaining and repairing the hydrological cycle necessary for fresh, safe, and abundant water supply. The current and future condition of our earth ecosystem is substantively dependent on individual and social decisions and policies associated with water. There is a need for (i) more coupled human-natural science to understand these relationships, (ii) greater public participation in connecting scientific facts and social values, and (iii) a willingness to innovate and adapt water and land use decisions and policies as scientific understanding and values evolve. C1 [Morton, Lois Wright] Iowa State Univ, Dept Sociol, Coll Agr & Life Sci, Ames, IA 50011 USA. [Morton, Lois Wright] USDA, NIFA Climate & Corn Based Cropping Syst CAP, New York, NY USA. RP Morton, LW (reprint author), Iowa State Univ, Dept Sociol, Coll Agr & Life Sci, Ames, IA 50011 USA. EM lwmorton@iastate.edu FU USDA-NIFA Integrated Water Program, Heartland Regional Water Coordination Initiative [2008-51130-19526]; USDA-NIFA Climate Change, Mitigation, and Adaptation in Corn-based Cropping Systems Coordinated Agricultural Project (CAP) [2011-68002-30190] FX Support for this work comes from USDA-NIFA Integrated Water Program award no. 2008-51130-19526 Heartland Regional Water Coordination Initiative and USDA-NIFA Climate Change, Mitigation, and Adaptation in Corn-based Cropping Systems Coordinated Agricultural Project (CAP) award no. 2011-68002-30190. NR 23 TC 2 Z9 2 U1 2 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2015 VL 107 IS 4 BP 1557 EP 1560 DI 10.2134/agronj14.0039 PG 4 WC Agronomy SC Agriculture GA CQ2UB UT WOS:000360456000038 ER PT J AU Karlen, DL Kovar, JL Birrell, SJ AF Karlen, Douglas L. Kovar, John L. Birrell, Stuart J. TI Corn Stover Nutrient Removal Estimates for Central Iowa, USA SO SUSTAINABILITY LA English DT Article DE plant nutrients; source carbon; fertilizer replacement costs; feedstock quality; EZ bales ID HARVEST; YIELD; FERTILITY AB One of the most frequent producer-asked questions to those persons striving to secure sustainable corn (Zea mays L.) stover feedstock supplies for Iowa's new bioenergy conversion or other bio-product facilities is what quantity of nutrients will be removed if I harvest my stover? Our objective is to summarize six years of field research from central Iowa, U.S.A. where more than 600, 1.5 m(2) samples were collected by hand and divided into four plant fractions: vegetative material from the ear shank upward (top), vegetative material from approximately 10 cm above the soil surface to just below the ear (bottom), cobs, and grain. Another 400 stover samples, representing the vegetative material collected directly from a single-pass combine harvesting system or from stover bales were also collected and analyzed. All samples were dried, ground, and analyzed to determine C, N, P, K, Ca, Mg, S, Al, B, Cu, Fe, Mn, and Zn concentrations. Mean concentration and dry matter estimates for each sample were used to calculate nutrient removal and estimate fertilizer replacement costs which averaged $25.06, $20.04, $16.62, $19.40, and $27.41 Mg-1 for top, bottom, cob, stover, and grain fractions, respectively. We then used the plant fraction estimates to compare various stover harvest scenarios and provide an answer to the producer question posed above. C1 [Karlen, Douglas L.; Kovar, John L.] USDA ARS, NLAE, Ames, IA 50011 USA. [Birrell, Stuart J.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. RP Karlen, DL (reprint author), USDA ARS, NLAE, 2110 Univ Blvd, Ames, IA 50011 USA. EM Doug.Karlen@ars.usda.gov; John.Kovar@ars.usda.gov; sbirrell@iastate.edu FU USDA-ARS National Laboratory for Agriculture and Environment (NLAE); Iowa State University (ISU) Agronomy and Agricultural Biosystems Engineering Research Center (AABERC); USDA-ARS Resilient Economic Agricultural Practices (REAP) project; Department of Energy (DOE) Bioenergy Technologies Office's Regional Feedstock Partnership Corn Stover Team under DOE award [DE-FC36-05GO85041] FX The authors recognize that this project could not have been accomplished without the full support and dedication of research support personnel associated with the USDA-ARS National Labora