FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Shepperd, WD Smith, FW Pelz, KA AF Shepperd, Wayne D. Smith, Frederick W. Pelz, Kristen A. TI Group Clearfell Harvest Can Promote Regeneration of Aspen Forests Affected by Sudden Aspen Decline in Western Colorado SO FOREST SCIENCE LA English DT Article DE Populus tremuloides; aspen regeneration; sudden aspen decline; coppice silviculture ID YELLOWSTONE-NATIONAL-PARK; POPULUS-TREMULOIDES; USA; DYNAMICS; ELK; CLIMATE AB An experimental assessment of the use of clearfell harvesting to initiate a regeneration response in commercially managed aspen forests affected by sudden aspen decline (SAD) was conducted in western Colorado in cooperation with the USDA Forest Service. Nine pure commercial quality aspen stands, with three levels of mortality attributed to SAD, were selected (three replicates per mortality level). Half of each stand was clearfelled, and half was left uncut. The aspen regeneration response was monitored for three growing seasons after harvest in the cut and uncut treatments. Cut treatments with low and moderate mortality had the best subsequent regeneration response, and those with the heaviest mortality exhibited the poorest regeneration response. Uncut treatments exhibited very little regeneration response, regardless of the initial overstory mortality level. Dead trees in the uncut overstory were projected to fall within 15 years. These results indicate that it is possible to successfully regenerate aspen forests affected by SAD, provided that treatment occurs before the majority of the aspen are dead. C1 [Shepperd, Wayne D.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Smith, Frederick W.; Pelz, Kristen A.] Colorado State Univ, Dept Forest & Range Stewardship, Ft Collins, CO 80523 USA. RP Shepperd, WD (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. EM Wayne.Shepperd@colostate.edu; skip@warnercnr.colostate.edu; kristen.pelz@colostate.edu FU USDA Forest Service FX This research was made possible with a grant from the USDA Forest Service and generous contributions of time and effort by the staff of the Grand Mesa, Uncompahgre, and Gunnison National Forests and the Paonia Ranger District. We thank Dr. James Worrall and two anonymas reviewers for their helpful suggestions to improve the article. NR 24 TC 2 Z9 2 U1 3 U2 5 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD OCT PY 2015 VL 61 IS 5 BP 932 EP 937 DI 10.5849/forsci.14-101 PG 6 WC Forestry SC Forestry GA CU2ND UT WOS:000363359300012 ER PT J AU Brown, JP AF Brown, John P. TI Spatial Allocation of Timber Product Output Roundwood Receipts SO FOREST SCIENCE LA English DT Article DE procurement; nonlinear mixed models; Gompertz function; logistic function; repeated measures ID WOOD PROCUREMENT; SOUTHERN AB Data from Georgia timber product output studies were used to develop models that spatially allocate roundwood receipts data from primary wood-using mills. Mill receipts data were converted to a cumulative frequency based on a distance from the mill to a county center. Distances were calculated as either straight line or shortest road distance, and county centers were determined as geographic centers or weighted by forest mass. Logistic and Gompertz nonlinear asymptotic model forms were used to model cumulative frequency of annual receipts within a repeated measures framework. It was determined that the number of mill employees and the shortest road distance were significant factors. The Gompertz model form predicted an unbiased cumulative frequency for the 2003 data using several spatial covariance types in the repeated measures analysis. These methods demonstrate the potential to spatially allocate a mill's total receipts to its surrounding counties in the absence of county-specific recording. C1 US Forest Serv, USDA, Princeton Forestry Sci Lab, Princeton, WV 24740 USA. RP Brown, JP (reprint author), US Forest Serv, USDA, Princeton Forestry Sci Lab, Princeton, WV 24740 USA. EM jpbrown@fs.fed.us NR 11 TC 0 Z9 0 U1 2 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD OCT PY 2015 VL 61 IS 5 BP 950 EP 958 DI 10.5849/forsci.14-123 PG 9 WC Forestry SC Forestry GA CU2ND UT WOS:000363359300014 ER PT J AU Tyson, GH McDermott, PF Li, C Chen, Y Tadesse, DA Mukherjee, S Bodeis-Jones, S Kabera, C Gaines, SA Loneragan, GH Edrington, TS Torrence, M Harhay, DM Zhao, SH AF Tyson, Gregory H. McDermott, Patrick F. Li, Cong Chen, Yuansha Tadesse, Daniel A. Mukherjee, Sampa Bodeis-Jones, Sonya Kabera, Claudine Gaines, Stuart A. Loneragan, Guy H. Edrington, Tom S. Torrence, Mary Harhay, Dayna M. Zhao, Shaohua TI WGS accurately predicts antimicrobial resistance in Escherichia coli SO JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY LA English DT Article ID UNITED-STATES; PLASMID; GENES; SALMONELLA; ENTEROBACTERIACEAE; SUSCEPTIBILITY; MUTATIONS; INTEGRONS; ANIMALS; STRAINS AB Objectives: The objective of this study was to determine the effectiveness of WGS in identifying resistance genotypes of MDR Escherichia coli and whether these correlate with observed phenotypes. Methods: Seventy-six E. coli strains were isolated from farm cattle and measured for phenotypic resistance to 15 antimicrobials with the Sensititre(R) system. Isolates with resistance to at least four antimicrobials in three classes were selected for WGS using an Illumina MiSeq. Genotypic analysis was conducted with in-house Perl scripts using BLAST analysis to identify known genes and mutations associated with clinical resistance. Results: Over 30 resistance genes and a number of resistance mutations were identified among the E. coli isolates. Resistance genotypes correlated with 97.8% specificity and 99.6% sensitivity to the identified phenotypes. The majority of discordant results were attributable to the aminoglycoside streptomycin, whereas there was a perfect genotype-phenotype correlation for most antibiotic classes such as tetracyclines, quinolones and phenicols. WGS also revealed information about rare resistance mechanisms, such as structural mutations in chromosomal copies of ampC conferring third-generation cephalosporin resistance. Conclusions: WGS can provide comprehensive resistance genotypes and is capable of accurately predicting resistance phenotypes, making it a valuable tool for surveillance. Moreover, the data presented here showing the ability to accurately predict resistance suggest that WGS may be used as a screening tool in selecting anti-infective therapy, especially as costs drop and methods improve. C1 [Tyson, Gregory H.; McDermott, Patrick F.; Li, Cong; Chen, Yuansha; Tadesse, Daniel A.; Mukherjee, Sampa; Bodeis-Jones, Sonya; Kabera, Claudine; Gaines, Stuart A.; Zhao, Shaohua] US FDA, Div Anim & Food Microbiol, Res Off, Ctr Vet Med, Laurel, MD USA. [Loneragan, Guy H.] Texas Tech Univ, Dept Anim & Food Sci, Int Ctr Food Ind Excellence, Lubbock, TX 79409 USA. [Edrington, Tom S.] USDA ARS, Food & Feed Safety Res Unit, College Stn, TX USA. [Torrence, Mary] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD USA. [Harhay, Dayna M.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Zhao, SH (reprint author), US FDA, Div Anim & Food Microbiol, Res Off, Ctr Vet Med, Laurel, MD USA. EM shaohua.zhao@fda.hhs.gov FU US Food and Drug Administration; Research Participation Program at the Center for Veterinary Medicine FX This work was supported by the US Food and Drug Administration with internal funds as part of routine work. This project was also supported in part by an appointment to the Research Participation Program at the Center for Veterinary Medicine administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the US Department of Energy and US Food and Drug Administration. NR 29 TC 19 Z9 19 U1 5 U2 15 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7453 EI 1460-2091 J9 J ANTIMICROB CHEMOTH JI J. Antimicrob. Chemother. PD OCT PY 2015 VL 70 IS 10 BP 2763 EP 2769 DI 10.1093/jac/dkv186 PG 7 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA CU0ZZ UT WOS:000363249800012 PM 26142410 ER PT J AU Campbell, JF Buckman, KA Fields, PG Subramanyam, B AF Campbell, James F. Buckman, Karrie A. Fields, Paul G. Subramanyam, Bhadriraju TI Evaluation of Structural Treatment Efficacy against Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) Using Meta-Analysis of Multiple Studies Conducted in Food Facilities SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tribolium castaneum; Tribolium confusum; flour mill; rice mill; fumigation ID STORED-PRODUCT INSECTS; METHYL-BROMIDE; FLOUR MILL; LIFE STAGES; ELEVATED-TEMPERATURES; SULFURYL FLUORIDE; HEAT-TREATMENT; FEED MILL; FUMIGATION; DYNAMICS AB The phase out of methyl bromide for the treatment of structures where grain is milled or processed has triggered a need to evaluate the effectiveness of alternative structural treatments such as sulfuryl fluoride and heat. Tribolium castaneum (Herbst) (red flour beetle) and Tribolium confusum Jacquelin du Val (confused flour beetle) (Coleoptera: Tenebrionidae) are primary targets for structural treatments, and impact of treatments on these species was evaluated in this study. Efficacy was measured by comparing beetle captures in pheromone- and kairomone-baited traps immediately before and immediately after treatments. Studies were conducted primarily in commercial wheat mills and rice mills, with the treatments conducted by commercial applicators. A meta-analysis approach was used to evaluate efficacy data collected from 111 treatments applied in 39 facilities. Findings confirm that structural fumigations and heat treatments greatly reduce pest populations within food facilities, but there was significant variation in the efficacy of individual treatments. Generally, the different treatment types (sulfuryl fluoride, methyl bromide, and heat) provided similar reductions in beetle capture using multiple metrics of beetle activity. The novel application of meta-analysis to structural treatment efficacy assessment generated a robust estimate of overall treatment efficacy, provided insights into factors potentially impacting efficacy, and identified data gaps that need further research. C1 [Campbell, James F.; Buckman, Karrie A.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Fields, Paul G.] Univ Manitoba, Cereal Res Ctr Engn, Agr & Agri Food Canada, Informat & Technol Complex, Winnipeg, MB R3T 5V6, Canada. [Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. RP Campbell, JF (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov FU United States Department of Agriculture National Institute of Food and Agriculture Methyl Bromide Transitions grants [2006-51102-03588, 2011-51102-31125] FX We thank the many people that provided information for inclusion in this analysis, from the management and workers at the different facilities that generously allowed us access, to the different people that provided the raw data that enabled this analysis to be completed. This research was funded in part by United States Department of Agriculture National Institute of Food and Agriculture Methyl Bromide Transitions grants 2006-51102-03588 and 2011-51102-31125. NR 46 TC 2 Z9 2 U1 1 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2125 EP 2140 DI 10.1093/jee/tov215 PG 16 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300001 PM 26453702 ER PT J AU Hou, LX Du, YL Johnson, JA Wang, SJ AF Hou, Lixia Du, Yanli Johnson, Judy A. Wang, Shaojin TI Thermal Death Kinetics of Conogethes Punctiferalis (Lepidoptera: Pyralidae) as Influenced by Heating Rate and Life Stage SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Conogethes punctiferalis; thermal treatment; life stage; heating rate; thermal death kinetics ID CODLING MOTH LEPIDOPTERA; RADIO-FREQUENCY TREATMENTS; YELLOW PEACH MOTH; FRUIT-FLY DIPTERA; TRANSITELLA WALKER LEPIDOPTERA; POMONELLA L. LEPIDOPTERA; INSECT CONTROL; QUARANTINE TREATMENT; SITOPHILUS-ORYZAE; HIGH-TEMPERATURE AB Thermal death kinetics of Conogethes punctiferalis (Guenee) (Lepidoptera: Pyralidae) at different life stages, heating rate, and temperature is essential for developing postharvest treatments to control pests in chestnuts. Using a heating block system (HBS), the most heat-tolerant life stage of C. punctiferalis and the effects of heating rate (0.1, 0.5, 1, 5, and 10 degrees C/min) on insect mortality were determined. The thermal death kinetic data of fifth-instar C. punctiferalis were obtained at temperatures between 44 and 50 degrees C at a heating rate of 5 degrees C/min. The results showed that the relative heat tolerance of C. punctiferalis was found to be fifth instars>pupae> third instars> eggs. To avoid the enhanced thermal tolerance of C. punctiferalis at low heating rates (0.1 or 0.5 degrees C/min), a high heating rate of 5 degrees C/min was selected to simulate the fast radio frequency heating in chestnuts and further determine the thermal death kinetic data. Thermal death curves of C. punctiferalis followed a 0th-order kinetic reaction model. The minimum exposure time to achieve 100% mortality was 55, 12, 6, and 3 min at 44, 46, 48, and 50 degrees C, respectively. The activation energy for controlling C. punctiferalis was 482.15 kJ/mol with the z value of 4.09 degrees C obtained from the thermal death-time curve. The information provided by thermal death kinetics for C. punctiferalis is useful in developing effective postharvest thermal treatment protocols for disinfesting chestnuts. C1 [Hou, Lixia; Wang, Shaojin] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Du, Yanli] Beijing Univ Agr, Plant Sci & Technol Coll, Beijing 102206, Peoples R China. [Johnson, Judy A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Wang, Shaojin] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Wang, SJ (reprint author), Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM shaojinwang@nwsuaf.edu.cn FU Open Fund for Key Laboratory of Physical Processing of Agricultural Product in Jiangsu Province [JAPP2013-2]; General Program of National Natural Science Foundation of China [31371853]; Shaanxi Agricultural Science and Technology Innovation and Research Project [2013K01-50] FX This research was conducted in the College of Mechanical and Electronic Engineering, Northwest A&F University, and supported by research grants from Open Fund for Key Laboratory of Physical Processing of Agricultural Product in Jiangsu Province (JAPP2013-2), General Program of National Natural Science Foundation of China (31371853), and Shaanxi Agricultural Science and Technology Innovation and Research Project (2013K01-50). NR 38 TC 0 Z9 0 U1 2 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2192 EP 2199 DI 10.1093/jee/tov194 PG 8 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300007 PM 26453708 ER PT J AU Light, DM Ovchinnikova, I Jackson, ES Haff, RP AF Light, Douglas M. Ovchinnikova, Inna Jackson, Eric S. Haff, Ronald P. TI Effects of X-Ray Irradiation on Male Navel Orangeworm Moths (Lepidoptera: Pyralidae) on Mating, Fecundity, Fertility, and Inherited Sterility SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Amyelois transitella; navel orangeworm; irradiation; x-ray; sterility ID INDIAN-MEAL MOTH; CACTOBLASTIS-CACTORUM LEPIDOPTERA; EPHESTIA-CAUTELLA LEPIDOPTERA; MEDITERRANEAN FLOUR MOTH; KUEHNIELLA ZELLER LEPIDOPTERA; ECTOMYELOIS-CERATONIAE ZELLER; ANGOUMOIS GRAIN MOTH; GAMMA-IRRADIATION; PLODIA-INTERPUNCTELLA; AMYELOIS-TRANSITELLA AB Male adult navel orangeworm, Amyelois transitella (Walker), were irradiated using a laboratory scale x-ray irradiation unit to determine the required dose for complete egg sterility of mated female moths and inherited sterility of F1 and F2 generations. Adult male A. transitella were irradiated in two separate experiments at 100-300 Gy and 50-175 Gy. Mating frequency, fecundity, and fertility of normal females crossed with irradiated parental males was compared with the mating of nonirradiated moths. Mating frequency was 100% for females crossed with nonirradiated control males. At male treatment doses of >= 150 Gy the percentage of females found unmated increased, while multiple-mated females decreased. Female fecundity was not affected while fertility was affected in a dose-dependent relationship to exposure of parental males to x-ray irradiation. Embryonic development of eggs to the prehatch stage and egg eclosion did not occur at radiation doses >= 125 Gy. Emergence of F1 adults was low and occurred only for progeny of parental males exposed to doses <= 100 Gy, with no emergence at >= 125 Gy. Though fecundity appeared similar for control and irradiated F1 females, no F2 eggs hatched for the test exposures of 50-100 Gy. Based on our results, a dose of >= 125 Gy had efficacy in inducing both primary parental sterility in treated male moths and inherited sterility in F1 male and female moths. Results suggest that A. transitella might be considered a candidate for the sterile insect technique using adults irradiated at these relatively low x-ray exposure doses. C1 [Light, Douglas M.; Ovchinnikova, Inna; Jackson, Eric S.; Haff, Ronald P.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Light, DM (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM doug.light@ars.usda.gov NR 90 TC 1 Z9 1 U1 0 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2200 EP 2212 DI 10.1093/jee/tov201 PG 13 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300008 PM 26453709 ER PT J AU Morales-Ramos, JA Rojas, MG AF Morales-Ramos, Juan A. Rojas, M. Guadalupe TI Effect of Larval Density on Food Utilization Efficiency of Tenebrio molitor (Coleoptera: Tenebrionidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE yellow mealworm; food conversion; rearing; biomass production; insects for food ID FREEMANI HINTON COLEOPTERA; IN-VIVO PRODUCTION; ENTOMOPATHOGENIC NEMATODES; NUTRIENT COMPOSITION; SELF-SELECTION; DIET; INSECTS; PENTATOMIDAE; PREY; INVERTEBRATES AB Crowding conditions of larvae may have a significant impact on commercial production efficiency of some insects, such as Tenebrio molitor L. (Coleoptera: Tenebrionidae). Although larval densities are known to affect developmental time and growth in T. molitor, no reports were found on the effects of crowding on food utilization. The effect of larval density on food utilization efficiency of T. molitor larvae was studied by measuring efficiency of ingested food conversion (ECI), efficiency of digested food conversion (EDC), and mg of larval weight gain per gram of food consumed (LWGpFC) at increasing larval densities (12, 24, 36, 48, 50, 62, 74, and 96 larvae per dm(2)) over four consecutive 3-wk periods. Individual larval weight gain and food consumption were negatively impacted by larval density. Similarly, ECI, ECD, and LWGpFC were negatively impacted by larval density. Larval ageing, measured as four consecutive 3-wk periods, significantly and independently impacted ECI, ECD, and LWGpFC in a negative way. General linear model analysis showed that age had a higher impact than density on food utilization parameters of T. molitor larvae. Larval growth was determined to be responsible for the age effects, as measurements of larval mass density (in grams of larvae per dm(2)) had a significant impact on food utilization parameters across ages and density treatments (in number of larvae per dm(2)). The importance of mass versus numbers per unit of area as measurements of larval density and the implications of negative effects of density on food utilization for insect biomass production are discussed. C1 [Morales-Ramos, Juan A.; Rojas, M. Guadalupe] USDA ARS, Natl Biol Control Lab, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. RP Morales-Ramos, JA (reprint author), USDA ARS, Natl Biol Control Lab, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. EM Juan.moralesramos@arsusda.gov NR 49 TC 1 Z9 1 U1 7 U2 51 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2259 EP 2267 DI 10.1093/jee/tov208 PG 9 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300013 PM 26453714 ER PT J AU Murua, MG Nagoshi, RN Dos Santos, DA Hay-Roe, MM Meagher, RL Vilardi, JC AF Gabriela Murua, M. Nagoshi, Rodney N. Dos Santos, Daniel A. Hay-Roe, Mirian M. Meagher, Robert L. Vilardi, J. C. TI Demonstration Using Field Collections that Argentina Fall Armyworm Populations Exhibit Strain-specific Host Plant Preferences SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE agricultural entomology; crop protection; ecology & population biology; pest management; population genetics ID SPODOPTERA-FRUGIPERDA LEPIDOPTERA; HELICOVERPA-ARMIGERA LEPIDOPTERA; BACILLUS-THURINGIENSIS; MOLECULAR CHARACTERIZATION; INSECT RESISTANCE; NATURAL REFUGE; BT CROPS; NOCTUIDAE; IDENTIFICATION; BRAZIL AB Spodoptera frugiperda, the fall armyworm, is a major economic pest throughout the Western Hemisphere of corn (maize), cotton, sorghum, and a variety of agricultural grasses and vegetable crops. Studies in the United States, the Caribbean, and Brazil demonstrated the existence of two subpopulations (previously designated "host strains") that differ in their choice of plant host. Specifically, the corn strain is preferentially found in corn and sorghum, while the rice strain is dominant in rice, turf grass, and alfalfa. However, inconsistent results were reported in surveys of fall armyworm in Argentina, with some indicating that the host plant preferences of the two strains might be compromised or even nonexistent. If correct, this would complicate efforts to control this pest by considerably expanding the range of habitats that would have to be considered as potential sources for fall armyworm infestations in specific crops. A reexamination of Argentine fall armyworm, this time with field collections rather than the laboratory colonies used in previous studies, confirmed the existence of the two strains and their host preferences. Specifically, the corn strain was consistently the majority population infesting corn and was usually so in sorghum, while the rice strain was predominant in pasture/turf grasses and alfalfa. The one outlier was a collection from rice, which had a corn strain majority. Overall, the data were generally consistent with strain behaviors observed in other areas of the Western Hemisphere. C1 [Gabriela Murua, M.] Estn Expt Agroind Obispo Colombres, Secc Zool Agr, San Miguel De Tucuman, Argentina. [Gabriela Murua, M.] Consejo Nacl Invest Cient & Tecn, EEAOC, ITANOA, Inst Tecnol Agroind Noroeste, RA-1033 Buenos Aires, DF, Argentina. [Nagoshi, Rodney N.; Hay-Roe, Mirian M.; Meagher, Robert L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32604 USA. [Dos Santos, Daniel A.] Univ Nacl Tucuman, Fac Cs Nat, IBN, CONICET, RA-4000 San Miguel De Tucuman, Argentina. [Vilardi, J. C.] Univ Buenos Aires, Fac Ciencias Exactas & Nat, CONICET, IEGEBA,Dept Ecol Genet & Evoluc,Lab Genet Poblac, Buenos Aires, DF, Argentina. RP Nagoshi, RN (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32604 USA. EM rodney.nagoshi@ars.usda.gov FU EEAOC; CONICET; Agencia Nacional de Promocion Cientifica y Tecnologica (PICT) [1910]; USDA-ARS; Consejo de Investigaciones de la Universidad Nacional de Tucuman (CIUNT) [G535/1]; National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-67003-30209] FX We thank Jean Thomas at USDA-ARS for excellent technical support and helpful comments on the manuscript, and Franco Scalora, Alejandro Vera, Carmen Uruena, Sofia Torres, and David Gonzalez at EEAOC for excellent technical support and assistance in the collection of material. We also thank Lic. Eduardo Willink (EEAOC) for constructive comments on an earlier draft of the manuscript and Dr. Fernando Navarro (CONICET, Instituto Superior de Entomologia Dr. Abraham Willink (INSUE), Fac. Cs. Naturales, Universidad Nacional de Tucuman) for the taxonomic identification of fall armyworm larvae and/or adults. This study was supported by EEAOC, CONICET, Agencia Nacional de Promocion Cientifica y Tecnologica (PICT #1910), USDA-ARS, Consejo de Investigaciones de la Universidad Nacional de Tucuman (CIUNT #G535/1), and the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Agreement No. 2011-67003-30209. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 43 TC 4 Z9 4 U1 3 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2305 EP 2315 DI 10.1093/jee/tov203 PG 11 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300018 PM 26453719 ER PT J AU Tillman, PG Khrimian, A Cottrell, TE Lou, X Mizell, RF Johnson, CJ AF Tillman, P. G. Khrimian, A. Cottrell, T. E. Lou, X. Mizell, R. F., III Johnson, C. J. TI Trap Cropping Systems and a Physical Barrier for Suppression of Stink Bugs (Hemiptera: Pentatomidae) in Cotton SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE pheromone-baited stink bug trap; trap cropping system; physical barrier; nectar provision ID NEZARA-VIRIDULA L.; HETEROPTERA-PENTATOMIDAE; TRICHOPODA-PENNIPES; SEASONAL ABUNDANCE; EUSCHISTUS-SERVUS; GEOMETRIC ISOMERS; SOUTH-CAROLINA; PECAN ORCHARDS; FARMSCAPES; CROPS AB Euschistus servus ( Say), Nezara viridula ( L.), and Chinavia hilaris ( Say) ( Hemiptera: Pentatomidae) are economic pests of cotton in the coastal plain of the southeastern United States. The objective of this 2- yr study was to determine the ability of trap cropping systems, pheromone- baited stink bug traps, and a synthetic physical barrier at the peanut- to- cotton interface to manage stink bugs in cotton. The physical barrier was the most effective management tactic. Stink bug density in cotton was lowest for this treatment. In 2010, boll injury was lower for the physical barrier compared to the other treatments except for soybean with stink bug traps. In 2011, boll injury was lower for this treatment compared to the control. Soybean was an effective trap crop, reducing both stink bug density in cotton and boll injury regardless if used alone or in combination with either stink bug traps or buckwheat. Incorporation of buckwheat in soybean enhanced parasitism of E. servus egg masses by Telenomus podisi Ashmead in cotton. The insertion of eyelets in the lid of the insect- collecting device of a stink bug trap allowed adult stink bug parasitoids, but not E. servus, to escape. Stand- alone stink bug traps were not very effective in deterring colonization of cotton by stink bugs or reducing boll injury. The paucity of effective alternative control measures available for stink bug management justifies further full- scale evaluations into these management tactics for control of these pests in crops. C1 [Tillman, P. G.; Johnson, C. J.] ARS, USDA, Crop Protect & Management Res Lab, Tifton, GA 31793 USA. [Khrimian, A.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Cottrell, T. E.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Lou, X.] Univ Georgia, Coll Agr & Environm Sci, Tifton, GA 31793 USA. [Mizell, R. F., III] Univ Florida, NFREC Quincy, Quincy, FL 32351 USA. RP Tillman, PG (reprint author), ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 57 TC 5 Z9 5 U1 0 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2324 EP 2334 DI 10.1093/jee/tov217 PG 11 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300020 PM 26453721 ER PT J AU Miller, DR Crowe, CM Mayo, PD Silk, PJ Sweeney, JD AF Miller, D. R. Crowe, C. M. Mayo, P. D. Silk, P. J. Sweeney, J. D. TI Responses of Cerambycidae and Other Insects to Traps Baited With Ethanol, 2,3-Hexanediol, and 3,2-Hydroxyketone Lures in North-Central Georgia SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cerambycidae; hardwood; detection; adventive; exotic ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; LONGHORN BEETLES COLEOPTERA; MULTIPLE-FUNNEL TRAPS; HOST-PLANT VOLATILES; AMBROSIA BEETLES; AGGREGATION PHEROMONE; GEOGRAPHIC-VARIATION; BARK BEETLE; ANOPLOPHORA-GLABRIPENNIS; ANAGLYPTUS-SUBFASCIATUS AB In north-central Georgia, 13 species of woodboring beetles (Coleoptera: Cerambycidae: Cerambycinae) were attracted to multiple-funnel traps baited with ethanol and one of the following pheromones: (1) racemic 3-hydroxyhexan-2-one; (2) racemic 3-hydroxyoctan-2-one; and (3) syn-2,3-hexanediol. The following species were attracted to traps baited with ethanol and 3-hydroxyhexan-2-one: Anelaphus pumilus (Newman), Eburia quadrigeminata (Say), Euderces pini (Olivier), Knulliana cincta (Drury), Neoclytus mucronatus (F.), Neoclytus scutellaris (Olivier), and Xylotrechus colonus (F.). Clytus marginicollis Castelnau & Gory, and Anelaphus parallelus (Newman) were attracted to traps baited with ethanol and 3-hydroxyoctan-2-one, whereas traps baited with ethanol and syn-2,3-hexanediol were attractive to Anelaphus villosus (F.), A. parallelus, Neoclytus acuminatus (F.), Neoclytus jouteli jouteli Davis, and Megacyllene caryae (Gahan). Ethanol enhanced catches of seven cerambycid species in traps baited with syn-2,3-hexanediol and 3,2-hydroxyketones. Catches of bark and ambrosia beetles (Curculionidae: Scolytinae) in ethanol-baited traps were largely unaffected by the addition of syn-2,3-hexanediol and 3,2-hydroxyketone lures, except for two species. The mean catches of Hypothenemus rotundicollis Wood & Bright and Dryoxylon onoharaensum (Murayama) in ethanol-baited traps increased and decreased, respectively, with the addition of racemic 3-hydroxyoctan-2-one. Traps baited with ethanol and syn-2,3-hexanediol were attractive to Xylobiops basilaris (Say) (Bostrichidae) and Chariessa pilosa (Forster) (Cleridae), whereas Temnoscheila virescens (F.) (Trogossitidae) were attracted to traps baited with ethanol and 3-hydroxyhexan-2-one. The assassin bug, Apiomerus crassipes (F.) (Hemiptera: Reduviidae), was attracted to traps baited with ethanol and 3,2-hydroxyketones. C1 [Miller, D. R.; Crowe, C. M.] USDA Forest Serv, Southern Res Stn, Athens, GA 30602 USA. [Mayo, P. D.; Silk, P. J.; Sweeney, J. D.] Nat Resources Canada, Canadian Forest Serv, Atlantic Forestry Ctr, Fredericton, NB E3B 5P7, Canada. RP Miller, DR (reprint author), USDA Forest Serv, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. EM dmiller03@fs.fed.us FU USDA-APHIS FX For assistance and permission to conduct these studies on their respective lands, we thank the staff at the Oconee National Forest and the Rock Eagle 4-H Center, Eatonton, GA. Species identifications were kindly verified by E. R. Hoebeke. Funding for this research was provided by USDA-APHIS. We thank A. M. Ray and two anonymous reviewers for critical reviews of the manuscript. The use of trade names and identification of firms or corporations does not constitute an official endorsement or approval by the governments of the United States or Canada of any product or service to the exclusion of others that may be suitable. The USDA is an equal opportunity provider and employer. NR 71 TC 2 Z9 2 U1 1 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2354 EP 2365 DI 10.1093/jee/tov220 PG 12 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300023 PM 26453724 ER PT J AU Islam, MS Barr, NB Braswell, WE Martinez, M Ledezma, LA Molongoski, J Mastro, V Schuenzel, EL AF Islam, M. S. Barr, N. B. Braswell, W. E. Martinez, M. Ledezma, L. A. Molongoski, J. Mastro, V. Schuenzel, E. L. TI A Multiplex Real-Time PCR Assay for Screening Gypsy Moths (Lepidoptera: Erebidae) in the United States for Evidence of an Asian Genotype SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Erebidae; gypsy moth; real-time PCR assay; Asian gypsy moth haplotype ID DISPAR L LEPIDOPTERA; LYMANTRIA-DISPAR; NORTH-AMERICA; IDENTIFICATION; INVASION; DNA AB European gypsy moth populations (Lymantria dispar L.) are well established and a proven destructive force in hardwood trees throughout the United States and Canada. Introduction of the exotic Asian gypsy moth into North America would be even more impactful, as Asian gypsy moth populations have wider host ranges, and are capable of naturally dispersing more rapidly due to female flight ability. To support early detection and exclusion of Asian gypsy moth, the U.S. Department of Agriculture (USDA) uses molecular techniques to screen moths trapped in North America for evidence of common Asian genotype. In order to strengthen U.S. domestic capacity to screen moths quickly and efficiently, we report a real-time PCR assay for this pest. A probe system using TaqMan 5' nuclease chemistry is reported for detection of an allele associated with common Asian gypsy moth genotypes. The targeted allele is located at the nuclear FS1 locus currently used by the USDA in conventional PCR tests to screen for evidence of Asian gypsy moth introductions or introgression. The diagnostic probe is successfully multiplexed with a conserved 18S probe system to detect reaction failure due to poor sample quality or quantity. The specificity, sensitivity, and repeatability of the FS1-18S multiplex real-time PCR assay were tested on laboratory-reared and field-collected moths to demonstrate diagnostic utility. Implications of the new assay as a screening tool for evidence of Asian gypsy moth introgression and introduction are discussed. C1 [Islam, M. S.; Martinez, M.; Schuenzel, E. L.] Univ Texas Pan Amer, Dept Biol, Edinburg, TX 78539 USA. [Barr, N. B.; Braswell, W. E.; Ledezma, L. A.] USDA APHIS, Ctr Plant Hlth Sci & Technol, Moore Air Base, Miss Labora, Edinburg, TX 78541 USA. [Molongoski, J.; Mastro, V.] USDA APHIS, Ctr Plant Hlth Sci & Technol, Otis Lab, Buzzards Bay, MA 02542 USA. [Schuenzel, E. L.] Univ Texas Rio Grande Valley, Dept Biol, Edinburg, TX 78539 USA. RP Schuenzel, EL (reprint author), Univ Texas Pan Amer, Dept Biol, 1201 Univ Dr, Edinburg, TX 78539 USA. EM erin.schuenzel@utrgv.edu FU U.S. Department of Agriculture-Animal Plant Health Inspection Services award [10-8100-0094-CA] FX Funding for the research supported by U.S. Department of Agriculture-Animal Plant Health Inspection Services award number 10-8100-0094-CA to E.L. Schuenzel. NR 32 TC 3 Z9 3 U1 1 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2015 VL 108 IS 5 BP 2450 EP 2457 DI 10.1093/jee/tov212 PG 8 WC Entomology SC Entomology GA CT5DQ UT WOS:000362828300033 PM 26453734 ER PT J AU Gomez-Chiarri, M Guo, XM Tanguy, A He, Y Proestou, D AF Gomez-Chiarri, Marta Guo, Ximing Tanguy, Arnaud He, Yan Proestou, Dina TI The use of -omic tools in the study of disease processes in marine bivalve mollusks SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Epigenomics; Genomics; Metagenomics; Proteomics; Bivalve mollusk; Host-pathogen ID OYSTER CRASSOSTREA-GIGAS; SCALLOP CHLAMYS-FARRERI; SYDNEY ROCK OYSTERS; EXPRESSED SEQUENCE TAGS; MUSSEL MYTILUS-GALLOPROVINCIALIS; EST-SSR MARKERS; RUDITAPES-PHILIPPINARUM HEMOCYTES; SINGLE NUCLEOTIDE POLYMORPHISMS; ARTIFICIAL CHROMOSOME LIBRARIES; ARGOPECTEN-IRRADIANS-IRRADIANS AB Our understanding of disease processes and host pathogen interactions in model species has benefited greatly from the application of medium and high-throughput genomic, metagenomic, epigenomic, transcriptomic, and proteomic analyses. The rate at which new, low-cost, high-throughput -omic technologies are being developed has also led to an expansion in the number of studies aimed at gaining a better understanding of disease processes in bivalves. This review provides a catalogue of the genetic and -omic tools available for bivalve species and examples of how -omics has contributed to the advancement of marine bivalve disease research, with a special focus in the areas of immunity, bivalve pathogen interactions, mechanisms of disease resistance and pathogen virulence, and disease diagnosis. The analysis of bivalve genomes and transcriptomes has revealed that many immune and stress-related gene families are expanded in the bivalve taxa examined thus far. In addition, the analysis of proteomes confirms that responses to infection are influenced by epigenetic, post-transcriptional, and post-translational modifications. The few studies performed in bivalves show that epigenetic modifications are non-random, suggesting a role for epigenetics in regulating the interactions between bivalves and their environments. Despite the progress -omic tools have enabled in the field of marine bivalve disease processes, there is much more work to be done. To date, only three bivalve genomes have been sequenced completely, with assembly status at different levels of completion. Transcriptome datasets are relatively easy and inexpensive to generate, but their interpretation will benefit greatly from high quality genome assemblies and improved data analysis pipelines. Finally, metagenomic, epigenomic, proteomic, and metabolomic studies focused on bivalve disease processes are currently limited but their expansion should be facilitated as more transcriptome datasets and complete genome sequences become available for marine bivalve species. (C) 2015 Elsevier Inc. All rights reserved. C1 [Gomez-Chiarri, Marta] Univ Rhode Isl, Dept Fisheries Anim & Vet Sci, Kingston, RI 02881 USA. [Guo, Ximing] Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA. [Tanguy, Arnaud] CNRS, UMR Adaptat & Diversite Milieu Mann 7144, Stn Biol Roscoff, F-29680 Roscoff, France. [Tanguy, Arnaud] Univ Paris 06, Sorbonne Univ, Stn Biol Roscoff, F-29680 Roscoff, France. [He, Yan] Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China. [Proestou, Dina] USDA ARS, Natl Cold Water Marine Aquaculture Ctr, Kingston, RI 02881 USA. RP Gomez-Chiarri, M (reprint author), Univ Rhode Isl, Dept Fisheries Anim & Vet Sci, 169 CBLS, Kingston, RI 02881 USA. EM gomezchi@uri.edu NR 235 TC 5 Z9 5 U1 12 U2 34 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD OCT PY 2015 VL 131 BP 137 EP 154 DI 10.1016/j.jip.2015.05.007 PG 18 WC Zoology SC Zoology GA CT8PA UT WOS:000363077300011 PM 26021714 ER PT J AU Kim, DK Lillehoj, HS Jang, SI Lee, SH Hong, YH Lamont, SJ AF Kim, Duk K. Lillehoj, Hyun S. Jang, Seung I. Lee, Sung H. Hong, Yeong H. Lamont, Susan J. TI k Genetically Disparate Fayoumi Chicken Lines Show Different Response to Avian Necrotic Enteritis SO JOURNAL OF POULTRY SCIENCE LA English DT Article DE disease resistance; Fayoumi; gene expression; NE; necrotic enteritis ID KAPPA-B-ZETA; CLOSTRIDIUM-PERFRINGENS; GENE-EXPRESSION; EIMERIA-MAXIMA; BROILER-CHICKENS; COMPLEX DISPARATE; RISK-FACTORS; PROTEIN; COCCIDIOSIS; INFECTIONS AB Necrotic enteritis (NE) has reemerged as a significant problem as a result of growing restrictions of antibiotics in agricultural animal production and increasing concerns over antibiotic resistance in human pathogens. To enhance our understanding of host-pathogen immunobiology in NE, transcriptional analysis was conducted to compare changes in NE-induced intestinal transcripts and to identify immune-related genes whose expression are associated with NE disease resistance using two genetically disparate Fayoumi chicken lines, M5.1 and M15.2. NE was induced by co-infection of Eimeria maxima and Clostridium perfringens using an established disease model and two major NE-induced clinical signs, body weight loss and intestinal lesions, were measured in two inbred Fayoumi chicken lines, M5.1 and M15.2. In the clinical criteria, line M5.1 chickens were more resistant to NE compared to line 15.2 birds. Although they have the same genetic background, these two chicken lines are genetically disparate at their major histocompatibility complex (MHC) and this difference was reflected in the differential expression patterns of several inflammatory genes such as suppressor of cytokine signaling 3 (SOCS3), interleukin 8 (IL8), nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta (NFKBIZ), serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 (SERPINF1), and gap junction protein, alpha 1, 43kDa (GJA1) between NE-afflicted and uninfected chickens. These results will lead to increased insights on the NE disease resistance mechanisms and the role of host genes controlling host immune response to C, perfringens. C1 [Kim, Duk K.; Lillehoj, Hyun S.; Jang, Seung I.; Lee, Sung H.] USDA, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Kim, Duk K.] C&K Genom Inc, Seoul 151919, South Korea. [Jang, Seung I.] Daejeon Metropolitan City, Inst Hlth & Environm, Taejon 302789, South Korea. [Lee, Sung H.] Rural Dev Adm, Nat Acad Agr Sci, Wanju Gun 565851, Jeollabuk Do, South Korea. [Hong, Yeong H.] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. [Lamont, Susan J.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. RP Lillehoj, HS (reprint author), USDA, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Bldg 1040,BARC East, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU ARS CRIS; Next-Generation BioGreen 21 Program [PJ 01104401, PJ00808402]; Rural Development Administration, Republic of Korea [PJ 01049004] FX The authors thank Margie Nichols and Stacy O'Donnell for their significant contribution to this research. This work was partially supported by ARS CRIS project and grants from the Next-Generation BioGreen 21 Program (No. PJ 01104401 and No. PJ00808402) and "Physiological activity and mechanism of action of Allium hookeri" (No. PJ 01049004), Rural Development Administration, Republic of Korea. NR 37 TC 2 Z9 2 U1 1 U2 3 PU JAPAN POULTRY SCIENCE ASSOC PI TSUKUBA PA C/O NATL INST LIVESTOCK & GLASSLAND SCIENCE, 2 IKENODAI, TSUKUBA, IBARAKI 305-0901, JAPAN SN 1346-7395 J9 J POULT SCI JI J. Poult. Sci. PD OCT PY 2015 VL 52 IS 4 BP 245 EP 252 DI 10.2141/jpsa.0140203 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CU2KW UT WOS:000363353400001 ER PT J AU Kondo, MC South, EC Branas, CC AF Kondo, Michelle C. South, Eugenia C. Branas, Charles C. TI Nature-Based Strategies for Improving Urban Health and Safety SO JOURNAL OF URBAN HEALTH-BULLETIN OF THE NEW YORK ACADEMY OF MEDICINE LA English DT Review DE Urban nature; Green space; Place-based interventions; Public health; Crime ID CORONARY-HEART-DISEASE; GREEN SPACE; NEIGHBORHOOD DISORDER; INNER-CITY; SOCIOECONOMIC-STATUS; PUBLIC-HEALTH; MENTAL-HEALTH; CARDIOVASCULAR-DISEASE; RESEARCH AGENDA; COMMON SPACES AB Place-based programs are being noticed as key opportunities to prevent disease and promote public health and safety for populations at-large. As one key type of place-based intervention, nature-based and green space strategies can play an especially large role in improving health and safety for dwellers in urban environments such as US legacy cities that lack nature and greenery. In this paper, we describe the current understanding of place-based influences on public health and safety. We focus on nonchemical environmental factors, many of which are related to urban abandonment and blight. We then review findings from studies of nature-based interventions regarding impacts on health, perceptions of safety, and crime. Based on our findings, we suggest that further research in this area will require (1) refined measures of green space, nature, and health and safety for cities, (2) interdisciplinary science and cross-sector policy collaboration, (3) observational studies as well as randomized controlled experiments and natural experiments using appropriate spatial counterfactuals and mixed methods, and (4) return-on-investment calculations of potential economic, social, and health costs and benefits of urban greening initiatives. C1 [Kondo, Michelle C.] Forest Serv, USDA, No Res Stn, Philadelphia, PA 19103 USA. [South, Eugenia C.] Univ Penn, Perelman Sch Med, Dept Emergency Med, Philadelphia, PA 19104 USA. [Branas, Charles C.] Univ Penn, Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. RP Kondo, MC (reprint author), Forest Serv, USDA, No Res Stn, 100 North 20th St,Ste 205, Philadelphia, PA 19103 USA. EM michelleckondo@fs.fed.us OI Kondo, Michelle/0000-0001-9152-1589 FU National Institutes of Health ([NIH] [R01AA020331]; Centers for Disease Control and Prevention ([CDC] [R49CE002474]; US Department of Agriculture (USDA), Forest Service FX This work was supported by the National Institutes of Health ([NIH] grant R01AA020331 to C. C. B.) and the Centers for Disease Control and Prevention ([CDC] grant R49CE002474), with additional funding from the US Department of Agriculture (USDA), Forest Service (to M. C. K.). The NIH and CDC had no role in designing or conducting the study, collecting, managing, analyzing, or interpreting the data, preparing, reviewing, or approving the article, or deciding to submit the article for publication. NR 130 TC 2 Z9 2 U1 12 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-3460 EI 1468-2869 J9 J URBAN HEALTH JI J. Urban Health PD OCT PY 2015 VL 92 IS 5 BP 800 EP 814 DI 10.1007/s11524-015-9983-y PG 15 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA CU1AW UT WOS:000363252400002 PM 26275455 ER PT J AU Pouyat, RV Szlavecz, K Yesilonis, ID Wong, CP Murawski, L Marra, P Casey, RE Lev, S AF Pouyat, R. V. Szlavecz, K. Yesilonis, I. D. Wong, C. P. Murawski, L. Marra, P. Casey, R. E. Lev, S. TI Multi-scale assessment of metal contamination in residential soil and soil fauna: A case study in the Baltimore-Washington metropolitan region, USA SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Earthworms; Heavy metals; Isopods; Soil; Urban; Urbanization gradient ID URBAN-RURAL GRADIENT; HEAVY-METALS; LEAD PB; BIOLOGICAL INDICATORS; SPATIAL-DISTRIBUTION; RISK-ASSESSMENT; SURFACE SOILS; NEW-YORK; LAND-USE; EARTHWORMS AB This study examined the distribution of metals in residential soils from the scale of a residential yard to a metropolitan area by comparing residences along an urbanization gradient in the Baltimore Washington area, USA. In addition, earthworms and terrestrial isopods were sampled from residential yards to measure body burdens of metals. Soil metal concentrations from lawns and planting bed (road, foundation, and yard) patches were compared (1) among land-use types (inner urban, outer urban, suburban, and rural); (2) between pre- and post-1940 built residential structures; and (3) among yard patch types. Lawn soil concentrations of As, Cd, and Pb varied statistically among the land-use types. Differences between inner urban and rural lawn soils varied almost eight-fold for Pb, three-fold for Cd, and more than two-fold for As. Bed patches exhibited a slightly stronger relationship than lawns across the urbanization gradient. A similar relationship was shown for pre- and post-1940 structures with older having higher concentrations than post-1940 structures. Earthworm body burdens were statistically correlated with soil Pb, while isopod burdens exhibited a significant relationship with soil As, Cr, Ni, Pb, and Zn. A post-hoc analysis with bird blood Pb data that was available for the residences, showed a significant relationship with earthworm Pb body burdens. This study suggests that despite policy efforts to reduce metal emissions, contamination of soil persists in urban residences at levels that have health implications for people and wildlife living in the Baltimore Washington, DC area. Published by Elsevier B.V. C1 [Pouyat, R. V.] USDA Forest Serv, Washington, DC 20250 USA. [Szlavecz, K.] Johns Hopkins Univ, Dept Earth & Planetary Sci, Baltimore, MD 21218 USA. [Yesilonis, I. D.] Univ Maryland, USDA Forest Serv, No Res Stn, Baltimore, MD 21227 USA. [Wong, C. P.; Murawski, L.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Marra, P.] Smithsonian Inst, Migratory Bird Ctr, Washington, DC 20008 USA. [Casey, R. E.; Lev, S.] Towson Univ, Urban Environm Biogeochem Lab, Towson, MD 21252 USA. RP Pouyat, RV (reprint author), USDA Forest Serv, Washington, DC 20250 USA. EM rpouyat@fs.fed.us FU USDA Forest Service's Northern Global Change Program and Research Work Unit NRS-8, Syracuse, NY; National Science Foundation [DEB-1027188]; University of Maryland Baltimore County, Center for Urban Environmental Research and Education grant from the U.S. Environmental Protection Agency [R-82818204] FX We thank C. O'Dea, S. Lerman and three anonymous reviewers for their comments on earlier drafts of this manuscript. Funding support was provided by the USDA Forest Service's Northern Global Change Program and Research Work Unit NRS-8, Syracuse, NY; supplemental grant for Research Experiences for Undergraduates (REU) to the Baltimore Ecosystem Study Long Term Ecological Research grant from the National Science Foundation (Grant no. DEB-1027188); and University of Maryland Baltimore County, Center for Urban Environmental Research and Education grant from the U.S. Environmental Protection Agency (R-82818204). The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture or the Forest Service of any product to the exclusion of others that may be suitable. NR 74 TC 5 Z9 5 U1 14 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD OCT PY 2015 VL 142 SI SI BP 7 EP 17 DI 10.1016/j.landurbplan.2015.05.001 PG 11 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA CU2JP UT WOS:000363350100002 ER PT J AU Ruiz-Gonzalez, A Cushman, SA Madeira, MJ Randi, E Gomez-Moliner, BJ AF Ruiz-Gonzalez, Aritz Cushman, Samuel A. Jose Madeira, Maria Randi, Ettore Gomez-Moliner, Benjamin J. TI Isolation by distance, resistance and/or clusters? Lessons learned from a forest-dwelling carnivore inhabiting a heterogeneous landscape SO MOLECULAR ECOLOGY LA English DT Review DE Bayesian analysis; isolation by cluster; isolation by distance; isolation by landscape resistance; landscape genetics; Martes martes; reciprocal causal modelling ID MARTEN MARTES-MARTES; MULTILOCUS GENOTYPE DATA; SPATIAL GENETIC-STRUCTURE; ALLELE FREQUENCY DATA; PINE MARTEN; POPULATION-STRUCTURE; COMPUTER-PROGRAM; MAXIMUM-LIKELIHOOD; AMERICAN MARTEN; HABITAT FRAGMENTATION AB Landscape genetics provides a valuable framework to understand how landscape features influence gene flow and to disentangle the factors that lead to discrete and/or clinal population structure. Here, we attempt to differentiate between these processes in a forest-dwelling small carnivore [European pine marten (Martes martes)]. Specifically, we used complementary analytical approaches to quantify the spatially explicit genetic structure and diversity and analyse patterns of gene flow for 140 individuals genotyped at 15 microsatellite loci. We first used spatially explicit and nonspatial Bayesian clustering algorithms to partition the sample into discrete clusters and evaluate hypotheses of isolation by barriers' (IBB). We further characterized the relationships between genetic distance and geographical (isolation by distance', IBD) and ecological distances (isolation by resistance', IBR) obtained from optimized landscape models. Using a reciprocal causal modelling approach, we competed the IBD, IBR and IBB hypotheses with each other to unravel factors driving population genetic structure. Additionally, we further assessed spatially explicit indices of genetic diversity using sGD across potentially overlapping genetic neighbourhoods that matched the inferred population structure. Our results revealed a complex spatial genetic cline that appears to be driven jointly by IBD and partial barriers to gene flow (IBB) associated with poor habitat and interspecific competition. Habitat loss and fragmentation, in synergy with past overharvesting and possible interspecific competition with sympatric stone marten (Martes foina), are likely the main factors responsible for the spatial genetic structure we observed. These results emphasize the need for a more thorough evaluation of discrete and clinal hypotheses governing gene flow in landscape genetic studies, and the potential influence of different limiting factors affecting genetic structure at different spatial scales. C1 [Ruiz-Gonzalez, Aritz; Jose Madeira, Maria; Gomez-Moliner, Benjamin J.] Univ Basque Country, UPV EHU, Dept Zool & Anim Cell Biol, Vitoria 01006, Spain. [Ruiz-Gonzalez, Aritz; Jose Madeira, Maria; Gomez-Moliner, Benjamin J.] Univ Basque Country, UPV EHU, Lascaray Res Ctr, Systemat Biogeog & Populat Dynam Res Grp, Vitoria 01006, Spain. [Ruiz-Gonzalez, Aritz; Randi, Ettore] Natl Inst Environm Protect & Res, ISPRA, Conservat Genet Lab, I-40064 Bologna, Italy. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Randi, Ettore] Aalborg Univ, Sect Environm Engn, Dept 18, DK-9000 Aalborg, Denmark. RP Ruiz-Gonzalez, A (reprint author), Univ Basque Country, UPV EHU, Dept Zool & Anim Cell Biol, C Paseo Univ 7, Vitoria 01006, Spain. EM aritz.ruiz@ehu.es OI GOMEZ MOLINER, BENJAMIN JUAN/0000-0002-3054-8806; RUIZ GONZALEZ, ARITZ/0000-0001-7409-4634 FU Basque Government through the Research group on 'Systematics, Biogeography and Population Dynamics' [IT317-10, GIC10/76, IT575/13]; SAIOTEK research programme [S-PE11UN028]; University of the Basque Country (UPV-EHU); Department of Environment, Territorial Planning, Agriculture and Fisheries (Basque Government) through IKT S.A under the University-Enterprise Research programme [UE07/02]; Dept. of Education Universities; Research of the Basque Government [DKR-2012-64]; Spanish Ministry of Environment, Regional Governments of Navarre and Aragon, Alava Provincial Council FX This study has been funded by the Basque Government through the Research group on 'Systematics, Biogeography and Population Dynamics' (Ref. IT317-10; GIC10/76; IT575/13), the SAIOTEK research programme (Ref: S-PE11UN028) and by the University of the Basque Country (UPV-EHU) and the Department of Environment, Territorial Planning, Agriculture and Fisheries (Basque Government) through IKT S.A under the University-Enterprise Research programme (Ref. UE07/02). Ruiz-Gonzalez holds a Post doc fellowship awarded by the Dept. of Education Universities and Research of the Basque Government (Ref. DKR-2012-64). Several samples analysed in this study have been obtained in the framework of different carnivore surveys funded by regional or national administrations (Spanish Ministry of Environment, Regional Governments of Navarre and Aragon, Alava Provincial Council). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. The authors wish to thank the following persons and institutions for supplying some of the tissue and faecal samples used in this study: Patricia Lizarraga, Ricardo Gutierrez and Laura Elorza (CRF Martioda-Alava Provincial Council); the technical staff and rangers of the Natural Parks of Alava (Juan Carlos Ortiz, Ricardo Ortiz, Maria Elena Garcia, Kepa Garcia, Sonia Benitez, Elisabeth Cabanillas, Iker Ayala, Arantza Puente, Jesus Gomez, Inaki Martinez, Lidia Lacha, Jokin Saez de Camara); Rangers from Alava (Asier Martinez, Tomas Landa, Javier Pinedo, Mario Corral) and Bizkaia (Igor Aginako, Julio Ruiz Guijarro, Ignacio Martinez, Eneko Diaz, Juan Manuel Perez de Ana, David Vado, Ander Eguia) Provincial Councils; Joseba Carreras (Alava Provincial Council); Jokin Larumbe (Navarre Government); Dr. Fermin Urra (GAVRN); Asun Gomez (TRAGSATEC); Dr. Madis Podra (Asoc. Vison Europeo); Javier Sesma and Txema Fernandez (IKT); Nerea Ruiz de Azua (EKOS, S.L.); NR 105 TC 6 Z9 6 U1 13 U2 65 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD OCT PY 2015 VL 24 IS 20 BP 5110 EP 5129 DI 10.1111/mec.13392 PG 20 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CU1IC UT WOS:000363273100004 PM 26394893 ER PT J AU Hu, XJ Roberts, DP Xie, LH Maul, JE Yu, CB Li, YS Zhang, YB Qin, L Liao, X AF Hu, Xiaojia Roberts, Daniel P. Xie, Lihua Maul, Jude E. Yu, Changbing Li, Yinshui Zhang, Yinbo Qin, Lu Liao, Xing TI Components of a Rice-Oilseed Rape Production System Augmented with Trichoderma sp Tr-1 Control Sclerotinia sclerotiorum on Oilseed Rape SO PHYTOPATHOLOGY LA English DT Article ID GROWTH-PROMOTING RHIZOBACTERIA; BACILLUS-SUBTILIS TU-100; BIOLOGICAL-CONTROL; PLANT-DISEASES; MEGATERIUM A6; BIOCONTROL; SOIL; RESISTANCE; WHEAT; FIELD AB Sclerotinia sclerotiorum causes serious yield losses on many crops throughout the world. A multicomponent treatment that consisted of the residual rice straw remaining after rice harvest and Trichoderma sp. Tri-1 (Tri-1) formulated with the oilseed rape seedcake fertilizer was used in field soil infested with S. sclerotiorum. This treatment resulted in oilseed rape seed yield that was significantly greater than the nontreated control or when the fungicide carbendizem was used in the presence of this pathogen in field trials. Yield data suggested that the rice straw, oilseed rape seedcake, and Tri-1 components of this treatment all contributed incrementally. Similar treatment results were obtained regarding reduction in disease incidence. Slight improvements in yield and disease incidence were detected when this multicomponent treatment was combined with a fungicide spray. Inhibition of sclerotial germination by this multicomponent treatment trended greater than the nontreated control at 90, 120, and 150 days in field studies but was not significantly different from this control. This multicomponent treatment resulted in increased yield relative to the nontreated control in the absence of pathogen in a greenhouse pot study, while the straw alone and the straw plus oilseed rape seedcake treatments did not; suggesting that Tri-1 was capable of promoting growth. Experiments reported here indicate that a treatment containing components of a rice-oilseed rape production system augmented with Tri-1 can control S. sclerotiorum on oilseed rape, be used in integrated strategies containing fungicide sprays for control of this pathogen, and promote plant growth. C1 [Hu, Xiaojia; Xie, Lihua; Yu, Changbing; Li, Yinshui; Zhang, Yinbo; Qin, Lu; Liao, Xing] Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Wuhan 430062, Peoples R China. [Roberts, Daniel P.; Maul, Jude E.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. RP Roberts, DP (reprint author), USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. EM dan.roberts@ars.usda.gov; liaox@oilcrops.cn FU Special Funds from the National Scientific Support Program of China [2010BAD01B09]; Introducing International Advanced Agricultural Science and Technology Program [2014-Z4]; Fundamental Research Incremental Program from Chinese Academy of Agricultural Sciences [2014ZL029] FX We thank B. Vinyard, Director, Statistics Group, USDA-ARS, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD 20705 for help with statistics. This work was funded by Special Funds from the National Scientific Support Program of China (contract number 2010BAD01B09), Introducing International Advanced Agricultural Science and Technology Program (contract number 2014-Z4), and Fundamental Research Incremental Program from Chinese Academy of Agricultural Sciences (contract number 2014ZL029). NR 70 TC 2 Z9 2 U1 4 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2015 VL 105 IS 10 BP 1325 EP 1333 DI 10.1094/PHYTO-12-14-0371-R PG 9 WC Plant Sciences SC Plant Sciences GA CT8RJ UT WOS:000363083400005 PM 26390095 ER PT J AU Briggs, J Chen, SS Zhang, WJ Nelson, S Dubcovsky, J Rouse, MN AF Briggs, Jordan Chen, Shisheng Zhang, Wenjun Nelson, Sarah Dubcovsky, Jorge Rouse, Matthew N. TI Mapping of SrTm4, a Recessive Stem Rust Resistance Gene from Diploid Wheat Effective to Ug99 SO PHYTOPATHOLOGY LA English DT Article ID F-SP TRITICI; PUCCINIA-GRAMINIS; HEXAPLOID WHEAT; BARBERRY ERADICATION; TETRAPLOID WHEATS; AEGILOPS-TAUSCHII; DURUM-WHEAT; RACE TTKSK; PH1 LOCUS; MONOCOCCUM AB Race TTKSK (or Ug99) of Puccinia graminis f. sp. tritici, the causal agent of wheat stem rust, is a serious threat to wheat production worldwide. Diploid wheat, Triticum monococcum (genome A(m)), has been utilized previously for the introgression of stem rust resistance genes Sr21, Sr22, and Sr35. Multipathotype seedling tests of biparental populations demonstrated that T monococcum accession PI 306540 collected in Romania contains a recessive resistance gene effective to all P. graminis f. sp. tritici races screened, including race TTKSK. We will refer to this gene as SrTm4, which is the fourth stem rust resistance gene characterized from T. monococcum. Using two mapping populations derived from crosses of PI 272557 x PI 306540 and G3116 x P1306540, we mapped SrTm4 on chromosome arm 2A(m)L within a 2.1 cM interval flanked by sequence-tagged markers BQ461276 and DR732348, which corresponds to a 240-kb region in Brachypodium chromosome 5. The eight microsatellite and nine sequence-tagged markers linked to SrTm4 will facilitate the introgression and accelerate the deployment of SrTm4-mediated Ug99 resistance in wheat breeding programs. C1 [Briggs, Jordan; Nelson, Sarah; Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Chen, Shisheng; Zhang, Wenjun; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Chen, Shisheng] Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. [Rouse, Matthew N.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Rouse, MN (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM matthew.rouse@ars.usda.gov RI Rouse, Matthew/G-5474-2011; Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU USDA-ARS National Plant Disease Recovery System; Durable Rust Resistance in Wheat project; Bill and Melinda Gates Foundation; United Kingdom Department for International Development; United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) [2011-68002-30029, 2012-67013-19401]; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation FX This research was supported by the USDA-ARS National Plant Disease Recovery System, the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation, and the United Kingdom Department for International Development. J. Dubcovsky acknowledges support from the National Research Initiative Competitive Grants 2011-68002-30029 (Triticeae-CAP) and 2012-67013-19401 from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA), and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. We acknowledge the Computational Genetics Laboratory of the University of Minnesota Supercomputing Institute for computational support. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 52 TC 1 Z9 1 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2015 VL 105 IS 10 BP 1347 EP 1354 DI 10.1094/PHYTO-12-14-0382-R PG 8 WC Plant Sciences SC Plant Sciences GA CT8RJ UT WOS:000363083400007 PM 25844826 ER PT J AU Mustafa, SG Spooner-Hart, R Duncan, M Pettis, JS Steidle, JLM Rosenkranz, P AF Mustafa, Sandra G. Spooner-Hart, Robert Duncan, Michael Pettis, Jeffery S. Steidle, Johannes L. M. Rosenkranz, Peter TI Age and aggregation trigger mating behaviour in the small hive beetle, Aethina tumida (Nitidulidae) SO SCIENCE OF NATURE LA English DT Article DE Aethina tumida; Mating behaviour; Aggregation; Pheromones; Cryptic female choice ID APIS-MELLIFERA-CAPENSIS; CRYPTIC FEMALE CHOICE; EUROPEAN HONEY-BEES; SEX-PHEROMONE; COLEOPTERA NITIDULIDAE; MURRAY COLEOPTERA; AGROTIS-IPSILON; LADYBIRD BEETLE; COLONIES; DISCRIMINATION AB This study aimed to investigate the poorly documented reproductive behaviour of the small hive beetle, Aethina tumida (Nitidulidae), a honey bee (Apis mellifera) parasite. We described the mating behaviour in detail and tested the hypothesis that beetle aggregation plays a vital role in mating in this species. Gender preference was examined in the context of age-dependency and possible chemical communication. Beetles started mating at a high frequency 18 days after emergence from the soil but only if they were aggregated (p<0.001); mating was infrequent when beetles were paired. Males in aggregation also tried to copulate with males and only copulated more frequently with females at 18 days after emergence from soil (p<0.001) in contrast to newly emerged, 7-day-old and 60-day-old beetles. Males and females spent more time in social contact with the opposite sex (p<0.01) when they were 18 days old in contrast to 7-day-old beetles. Filter papers which had been in contact with 21-day-old beetles were highly attractive to similar-aged beetles of the opposite sex (p<0.01). This suggests that chemical substances produced by the beetles themselves play a role in mating. Mating behaviour was characterised by a short precopulation courtship and female aggression towards other females and copulating couples. Both behaviours may be indicative of cryptic female choice. Delayed onset of reproductive behaviour is typical of many polygamous species, whilst the indispensability of aggregation for onset of sexual behaviour seems to be a feature unique to A. tumida. Both strategies support mass reproduction in this parasitic species, enabling A. tumida to overcome its honey bee host colony, and are probably triggered by chemotactic cues. C1 [Mustafa, Sandra G.; Rosenkranz, Peter] Univ Hohenheim, Apicultural State Inst, D-70599 Stuttgart, Germany. [Spooner-Hart, Robert; Duncan, Michael] Univ Western Sydney, Sch Sci & Hlth, Penrith, NSW 2751, Australia. [Pettis, Jeffery S.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Steidle, Johannes L. M.] Univ Hohenheim, Inst Zool, D-70599 Stuttgart, Germany. RP Mustafa, SG (reprint author), Univ Hohenheim, Apicultural State Inst, August von Hartmannstr 13, D-70599 Stuttgart, Germany. EM s.mustafa@gmx.net FU German Federal Ministry for Food, Agriculture and Consumer Protection through the Federal Agency for Agriculture and Food FX The authors are particularly grateful to the whole staff of the Centre for Plant and Food Science (now School of Science and Health) at the University of Western Sydney and the USDA-ARS Bee Research Laboratory for supporting us during our visits on all levels. Furthermore, we thank Professor Bessei for providing unrestricted access to a computer equipped with the Observer 0.5 Software. Financial support was provided by the German Federal Ministry for Food, Agriculture and Consumer Protection through the Federal Agency for Agriculture and Food. NR 68 TC 0 Z9 0 U1 4 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0028-1042 EI 1432-1904 J9 SCI NAT-HEIDELBERG JI Sci. Nat. PD OCT PY 2015 VL 102 IS 9-10 AR 49 DI 10.1007/s00114-015-1300-9 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CT8EC UT WOS:000363046800004 ER PT J AU Biresaw, G Bantchev, GB AF Biresaw, Girma Bantchev, Grigor B. TI Tribological Properties of Limonene Bisphosphonates SO TRIBOLOGY LETTERS LA English DT Article DE Limonene; Bisphosphonates; Chemical modification; Synthesis; Polarity; Four-ball tribometer; Anti-wear; Extreme pressure; Weld point; Coefficient of friction; Wear scar diameter; PDSC; Onset temperature; Peak temperature; GC-MS; NMR; FTIR ID SEED OILS; LUBRICANTS AB Limonene was chemically modified by reacting it with dialkyl phosphites of varying alkyl structures under inert atmosphere in the presence of free radical initiators. The reaction gave a mixture of mono-and di-adduct products and was optimized to produce only the di-adduct product limonene bisphosphonate by forcing both limonene double bonds to react completely. The product mixture was carefully characterized using a combination of gas chromatography-mass spectroscopy, infrared spectroscopy, and nuclear magnetic resonance spectroscopy (H-1, C-13, P-31). The bisphosphonates were investigated for their physical and tribological properties. The alkyl bisphosphonates displayed density and viscosity that was a function of the alkyl structure (methyl, ethyl, n-butyl) and much higher than the values for the unreacted limonene. They also displayed improved oxidation stability but lower viscosity index and solubility in polyalphaolefin (PAO6) and high-oleic sunflower oil (HOSuO) base oils. Tribological characterization of the neat modified oils on a four-ball tribometer showed improved extreme-pressure weld point by all three di-adducts and improved anti-wear coefficient of friction (COF) and wear scar diameter (WSD) by the n-butyl di-adduct only. The limonene bisphosphonates also displayed improved COF and WSD as additives in PAO6 and HOSuO base oils at low concentrations. The effects of chemical modifications on physical and tribological properties can be explained in terms of increased polarity of the modified products, insertion of heavy atoms (from PO3) into the limonene structure, and complete absence of unsaturation in the modified products. C1 [Biresaw, Girma; Bantchev, Grigor B.] ARS, USDA, Biooils Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Biresaw, G (reprint author), ARS, USDA, Biooils Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM girma.biresaw@ars.usda.gov OI Bantchev, Grigor/0000-0003-2790-5195 NR 69 TC 1 Z9 1 U1 2 U2 7 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1023-8883 EI 1573-2711 J9 TRIBOL LETT JI Tribol. Lett. PD OCT PY 2015 VL 60 IS 1 AR 11 DI 10.1007/s11249-015-0578-2 PG 15 WC Engineering, Chemical; Engineering, Mechanical SC Engineering GA CT8FV UT WOS:000363051300002 ER PT J AU Meyer, SE Pendleton, BK AF Meyer, Susan E. Pendleton, Burton K. TI Evolutionary drivers of mast-seeding in a long-lived desert shrub SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE blackbrush; Coleogyne ramosissima; mass flowering; predator satiation; resource matching; wind pollination efficiency ID BLACKBRUSH COLEOGYNE-RAMOSISSIMA; WIND POLLINATION; SEEDLING ESTABLISHMENT; WESTERN-AUSTRALIA; FRUIT PRODUCTION; ROSACEAE; TREES; GERMINATION; SYNCHRONY; PATTERNS AB PREMISE OF THE STUDY: The evolutionary drivers and proximal regulators of mast-seeding are well understood for species of mesic environments, but how these regulators interact with high spatial and interannual variability in growing-season precipitation for a masting species in a desert environment has never been examined. METHOD: We followed flowering and seed production in 16 populations of the North American desert shrub blackbrush (Coleogyne ramosissima) from contrasting environments across its range over an 11-year period to determine patterns of interannual reproductive output variation. KEY RESULT: Patterns of reproductive output in blackbrush did not track current growing season precipitation, but instead were regulated by prior-year weather cues. The strength of the response to the masting cue depended on habitat quality, with higher mean reproductive output, shorter intervals between years of high seed production, and lower CVp at more favorable sites. Wind pollination efficiency was demonstrated to be an important evolutionary driver of masting in blackbrush, and satiation of heteromyid seed predator-dispersers was supported as an evolutionary driver based on earlier studies. CONCLUSIONS: Both the evolutionary drivers and proximal regulators of masting in blackbrush are similar to those demonstrated for masting species of mesic environments. Relatively low synchrony across populations in response to regional masting cues occurs at least partly because prior-year environmental cues can trigger masting efforts in years with resource limitation due to suboptimal precipitation, especially in more xeric low-elevation habitats. C1 [Meyer, Susan E.] US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. [Pendleton, Burton K.] US Forest Serv, Rocky Mt Res Stn, Albuquerque, NM 87102 USA. RP Meyer, SE (reprint author), US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, 735 N 500 E, Provo, UT 84606 USA. EM semeyer@xmission.com NR 33 TC 4 Z9 4 U1 2 U2 18 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 EI 1537-2197 J9 AM J BOT JI Am. J. Bot. PD OCT PY 2015 VL 102 IS 10 BP 1666 EP 1675 DI 10.3732/ajb.1500209 PG 10 WC Plant Sciences SC Plant Sciences GA CT5NA UT WOS:000362855900011 PM 26451036 ER PT J AU Geng, P Zhang, ML Harnly, JM Luthria, DL Chen, P AF Geng, Ping Zhang, Mengliang Harnly, James M. Luthria, Devanand L. Chen, Pei TI Use of fuzzy chromatography mass spectrometric (FCMS) fingerprinting and chemometric analysis for differentiation of whole-grain and refined wheat (T-aestivum) flour SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE UHPLC; PCA; SIMCA; PLS; Phospholipids; Flavonoids ID LEAST-SQUARES REGRESSION; FAST-ATOM-BOMBARDMENT; CARDIOVASCULAR-DISEASE; ANTIOXIDANT ACTIVITY; DIETARY-SUPPLEMENTS; PATTERN-RECOGNITION; FLAVONOIDS; RISK; PHYTOCHEMICALS; VARIETIES AB A fuzzy chromatography mass spectrometric (FCMS) fingerprinting method combined with chemometric analysis has been established for rapid discrimination of whole-grain flour (WF) from refined wheat flour (RF). Bran, germ, endosperm, and WF from three local cultivars or purchased from a grocery store were studied. The state of refinement (whole vs. refined) of wheat flour was differentiated successfully by use of principal-components analysis (PCA) and soft independent modeling of class analogy (SIMCA), despite potential confounding introduced by wheat class (red vs. white; hard vs. soft) or resources (different brands). Twelve discriminatory variables were putatively identified. Among these, dihexoside, trihexoside, apigenin glycosides, and citric acid had the highest peak intensity for germ. Variable line plots indicated phospholipids were more abundant in endosperm. Samples of RF and WF from three cultivars (Hard Red, Hard White, and Soft White) were physically mixed to furnish 20, 40, 60, and 80 % WF of each cultivar. SIMCA was able to discriminate between 100 %, 80 %, 60 %, 40 %, and 20 % WF and 100 % RF. Partial least-squares (PLS) regression was used for prediction of RF-to-WF ratios in the mixed samples. When PLS models were used the relative prediction errors for RF-to-WF ratios were less than 6 %. C1 [Geng, Ping; Zhang, Mengliang; Harnly, James M.; Luthria, Devanand L.; Chen, Pei] USDA ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. RP Chen, P (reprint author), USDA ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, Bldg 161,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov RI Zhang, Mengliang/J-3573-2015 OI Zhang, Mengliang/0000-0001-8366-0052 NR 36 TC 2 Z9 2 U1 0 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 EI 1618-2650 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD OCT PY 2015 VL 407 IS 26 BP 7875 EP 7888 DI 10.1007/s00216-015-9007-5 PG 14 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CT0UM UT WOS:000362512800004 PM 26374564 ER PT J AU Meng-Reiterer, J Varga, E Nathanail, AV Bueschl, C Rechthaler, J McCormick, SP Michlmayr, H Malachova, A Fruhmann, P Adam, G Berthiller, F Lemmens, M Schuhmacher, R AF Meng-Reiterer, Jacqueline Varga, Elisabeth Nathanail, Alexis V. Bueschl, Christoph Rechthaler, Justyna McCormick, Susan P. Michlmayr, Herbert Malachova, Alexandra Fruhmann, Philipp Adam, Gerhard Berthiller, Franz Lemmens, Marc Schuhmacher, Rainer TI Tracing the metabolism of HT-2 toxin and T-2 toxin in barley by isotope-assisted untargeted screening and quantitative LC-HRMS analysis SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE Untargeted metabolite profiling; Stable isotopic labelling; Fast polarity switching; Type A trichothecenes; Masked mycotoxins ID RESOLUTION MASS-SPECTROMETRY; MASKED MYCOTOXINS; METABOLOMICS; WHEAT; DEOXYNIVALENOL; TRICHOTHECENES; GLUCOSIDES; EXTRACTION; DIGESTION; SOFTWARE AB An extensive study of the metabolism of the type A trichothecene mycotoxins HT-2 toxin and T-2 toxin in barley using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) is reported. A recently developed untargeted approach based on stable isotopic labelling, LC-Orbitrap-MS analysis with fast polarity switching and data processing by MetExtract software was combined with targeted LC-Q-TOF-MS(/MS) analysis for metabolite structure elucidation and quantification. In total, 9 HT-2 toxin and 13 T-2 toxin metabolites plus tentative isomers were detected, which were successfully annotated by calculation of elemental formulas and further LC-HRMS/MS measurements as well as partly identified with authentic standards. As a result, glucosylated forms of the toxins, malonylglucosides, and acetyl and feruloyl conjugates were elucidated. Additionally, time courses of metabolite formation and mass balances were established. For absolute quantification of those compounds for which standards were available, the method was validated by determining apparent recovery, signal suppression, or enhancement and extraction recovery. Most importantly, T-2 toxin was rapidly metabolised to HT-2 toxin and for both parent toxins HT-2 toxin-3-O-beta-glucoside was identified (confirmed by authentic standard) as the main metabolite, which reached its maximum already 1 day after toxin treatment. C1 [Meng-Reiterer, Jacqueline; Varga, Elisabeth; Bueschl, Christoph; Malachova, Alexandra; Berthiller, Franz; Schuhmacher, Rainer] Univ Nat Resources & Life Sci, Dept Agrobiotechnol IFA Tulln, Ctr Analyt Chem, Vienna BOKU, A-3430 Tulln, Austria. [Meng-Reiterer, Jacqueline; Lemmens, Marc] BOKU, IFA Tulln, Inst Biotechnol Plant Prod, A-3430 Tulln, Austria. [Varga, Elisabeth; Malachova, Alexandra; Berthiller, Franz] BOKU, IFA Tulln, Christian Doppler Lab Mycotoxin Metab, A-3430 Tulln, Austria. [Nathanail, Alexis V.] Finnish Food Safety Author Evira, Res & Lab Dept, Chem & Toxicol Unit, Helsinki 00790, Finland. [Rechthaler, Justyna] Univ Appl Sci Wr Neustadt, Degree Programme Biotech Proc FHWN Tulln, A-3430 Tulln, Austria. [McCormick, Susan P.] USDA, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. [Michlmayr, Herbert; Fruhmann, Philipp; Adam, Gerhard] BOKU, Dept Appl Genet & Cell Biol, A-3430 Tulln, Austria. [Fruhmann, Philipp] Vienna Univ Technol, Inst Appl Synthet Chem, A-1060 Vienna, Austria. RP Schuhmacher, R (reprint author), Univ Nat Resources & Life Sci, Dept Agrobiotechnol IFA Tulln, Ctr Analyt Chem, Vienna BOKU, Konrad Lorenz Str 20, A-3430 Tulln, Austria. EM rainer.schuhmacher@boku.ac.at OI Fruhmann, Philipp/0000-0001-9165-5202; Varga, Elisabeth/0000-0001-6046-3259 FU Austrian Science Fund (FWF) [P26213]; Austrian Federal Ministry of Science, Research and Economy; National Foundation of Research, Technology; BIOMIN Holding GmbH; Nestec Ltd.; Vienna Science and Technology Fund [LS12-012]; FWF [SFB F37]; Finnish Funding Agency for Technology and Innovation (TEKES) [401/31/2011] FX We are grateful to I. Maloku for plant cultivation and N. Kampleitner for his support in Orbitrap measurements. Special thank goes to the Austrian Science Fund (FWF) for financing major parts of this work (Project P26213). Furthermore, we want to thank the Austrian Federal Ministry of Science, Research and Economy, the National Foundation of Research, Technology, BIOMIN Holding GmbH and Nestec Ltd. for funding the Christian Doppler Laboratory for Mycotoxin Metabolism. The HT2-3-O-beta-Glc was produced within the Vienna Science and Technology Fund project LS12-012, and the 3-acetyl-T2 was synthesised within the FWF project SFB F37. The SFB F37 project also enabled the development of MetExtract. Finally, the Finnish Funding Agency for Technology and Innovation (TEKES) is acknowledged for funding the Myco-DETECT project (401/31/2011). NR 32 TC 11 Z9 11 U1 8 U2 32 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 EI 1618-2650 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD OCT PY 2015 VL 407 IS 26 BP 8019 EP 8033 DI 10.1007/s00216-015-8975-9 PG 15 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CT0UM UT WOS:000362512800018 PM 26335000 ER PT J AU Meerow, AW Noblick, L Salas-Leiva, DE Sanchez, V Francisco-Ortega, J Jestrow, B Nakamura, K AF Meerow, Alan W. Noblick, Larry Salas-Leiva, Dayana E. Sanchez, Vanessa Francisco-Ortega, Javier Jestrow, Brett Nakamura, Kyoko TI Phylogeny and historical biogeography of the cocosoid palms (Arecaceae, Arecoideae, Cocoseae) inferred from sequences of six WRKY gene family loci SO CLADISTICS LA English DT Article ID SPECIES TREES; TRANSCRIPTION FACTORS; DNA-SEQUENCES; DIVERSIFICATION PATTERNS; RECOMBINANT SEQUENCES; GLOBAL BIOGEOGRAPHY; MAXIMUM-LIKELIHOOD; COMPUTER-PROGRAM; MULTILOCUS DATA; NORTHERN ANDES AB Arecaceae tribe Cocoseae is the most economically important tribe of palms, including both coconut and African oil palm. It is mostly represented in the Neotropics, with one and two genera endemic to South Africa and Madagascar, respectively. Using primers for six single copy WRKY gene family loci, we amplified DNA from 96 samples representing all genera of the palm tribe Cocoseae as well as outgroup tribes Reinhardtieae and Roystoneae. We compared parsimony (MP), maximum likelihood (ML), and Bayesian (13) analysis of the supermatrix with three species-tree estimation approaches. Subtribe Elaeidinae is sister to the Bactridinae in all analyses. Within subtribe Attaleinae, Lytocaryum, previously nested in Syagrus, is now positioned by MP and ML as sister to the former, with high support B maintains Lytocarvum embedded within Syagrus. Both MP and ML resolve Cocos as sister to Syagrus; B positions Cocos as sister to Attalea. Bactridineae is composed of two sister clades, Bactarts and Desmoncus in one, for which there is morphological support, and a second comprising Acrocomia, Astrocaryum, and Aiphanes. Two B and one NIL gene tree-species estimation approaches are incongruent with the supermatrix in a few critical intergeneric clades, but resolve the same infrageneric relationships. The biogeographic history of the Cocoseae is dominated by dispersal events. The tribe originated in the late Cretaceous in South America. Evaluated together, the supermatrix and species tree analyses presented in this paper provide the most accurate picture of the evolutionary history of the tribe to date, with more congruence than incongruence among the various methodologies. Published [2014]. This article is a U.S. Government work and is in the public domain in the USA. C1 [Meerow, Alan W.; Salas-Leiva, Dayana E.; Sanchez, Vanessa; Nakamura, Kyoko] ARS, USDA, SHRS, Natl Germplasm Repository, Miami, FL 33158 USA. [Noblick, Larry; Salas-Leiva, Dayana E.] Montgomery Bot Ctr, Coral Gables, FL 33156 USA. [Salas-Leiva, Dayana E.; Francisco-Ortega, Javier] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA. [Francisco-Ortega, Javier; Jestrow, Brett] Fairchild Trop Bot Garden, Kushlan Trop Sci Inst, Miami, FL 33156 USA. RP Meerow, AW (reprint author), ARS, USDA, SHRS, Natl Germplasm Repository, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM alan.meerow@ars.usda.gov NR 160 TC 5 Z9 7 U1 4 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0748-3007 EI 1096-0031 J9 CLADISTICS JI Cladistics PD OCT PY 2015 VL 31 IS 5 BP 509 EP 534 DI 10.1111/cla.12100 PG 26 WC Evolutionary Biology SC Evolutionary Biology GA CT5IP UT WOS:000362842500004 ER PT J AU Guo, QF Fei, SL Dukes, JS Oswalt, CM Iannone, BV Potter, KM AF Guo, Qinfeng Fei, Songlin Dukes, Jeffrey S. Oswalt, Christopher M. Iannone, Basil V., III Potter, Kevin M. TI A unified approach for quantifying invasibility and degree of invasion SO ECOLOGY LA English DT Article DE abundance; community ecology theory; competition; invasion theory; niche availability; prediction; resources ID SPECIES RICHNESS; BIOLOGICAL INVASIONS; PROPAGULE PRESSURE; PLANT-COMMUNITIES; DIVERSITY; BIODIVERSITY; RESISTANCE; GRASSLAND; COMPETITION; HYPOTHESES AB Habitat invasibility is a central focus of invasion biology, with implications for basic ecological patterns and processes and for effective invasion management. "Invasibility'' is, however, one of the most elusive metrics and misused terms in ecology. Empirical studies and meta-analyses of invasibility have produced inconsistent and even conflicting results. This lack of consistency, and subsequent difficulty in making broad cross-habitat comparisons, stem in part from (1) the indiscriminant use of a closely related, but fundamentally different concept, that of degree of invasion (DI) or level of invasion; and (2) the lack of common invasibility metrics, as illustrated by our review of all invasibility-related papers published in 2013. To facilitate both cross-habitat comparison and more robust ecological generalizations, we clarify the definitions of invasibility and DI, and for the first time propose a common metric for quantifying invasibility based on a habitat's resource availability as inferred from relative resident species richness and biomass. We demonstrate the feasibility of our metric using empirical data collected from 2475 plots from three forest ecosystems in the eastern United States. We also propose a similar metric for DI. Our unified, resource-based metrics are scaled from 0 to 1, facilitating cross-habitat comparisons. Our proposed metrics clearly distinguish invasibility and DI from each other, which will help to (1) advance invasion ecology by allowing more robust testing of generalizations and (2) facilitate more effective invasive species control and management. C1 [Guo, Qinfeng] US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, Res Triangle Pk, NC 27709 USA. [Fei, Songlin; Dukes, Jeffrey S.; Iannone, Basil V., III] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Oswalt, Christopher M.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN 37919 USA. [Potter, Kevin M.] N Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC 27709 USA. RP Guo, QF (reprint author), US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, 3041 East Cornwallis Rd, Res Triangle Pk, NC 27709 USA. EM qguo@fs.fed.us RI Dukes, Jeffrey/C-9765-2009; OI Dukes, Jeffrey/0000-0001-9482-7743; Fei, Songlin/0000-0003-2772-0166 FU NSF MacroSystems grant [DEB-1241932] FX We thank the reviewers for helpful comments. This work is supported by a NSF MacroSystems grant (DEB-1241932). NR 53 TC 6 Z9 6 U1 11 U2 63 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD OCT PY 2015 VL 96 IS 10 BP 2613 EP 2621 DI 10.1890/14-2172.1.sm PG 9 WC Ecology SC Environmental Sciences & Ecology GA CT5MD UT WOS:000362853600006 PM 26649383 ER PT J AU Ford, AT Goheen, JR Augustine, DJ Kinnaird, MF O'Brien, TG Palmer, TM Pringle, RM Woodroffe, R AF Ford, Adam T. Goheen, Jacob R. Augustine, David J. Kinnaird, Margaret F. O'Brien, Timothy G. Palmer, Todd M. Pringle, Robert M. Woodroffe, Rosie TI Recovery of African wild dogs suppresses prey but does not trigger a trophic cascade SO ECOLOGY LA English DT Article DE Acacia; African wild dogs (Lycaon pictus); antelope; dik-dik (Madoqua guentheri); endangered species; food web; indirect effect; large carnivore; rain; savanna; tree cover ID SAVING YELLOWSTONE ASPEN; LANDSCAPE-LEVEL TEST; PREDATION RISK; COMMUNITY STRUCTURE; RESOURCE EDIBILITY; LYCAON-PICTUS; BODY-SIZE; FOOD-WEB; WOLVES; POPULATION AB Increasingly, the restoration of large carnivores is proposed as a means through which to restore community structure and ecosystem function via trophic cascades. After a decades-long absence, African wild dogs (Lycaon pictus) recolonized the Laikipia Plateau in central Kenya, which we hypothesized would trigger a trophic cascade via suppression of their primary prey (dik-dik, Madoqua guentheri) and the subsequent relaxation of browsing pressure on trees. We tested the trophic-cascade hypothesis using (1) a 14-year time series of wild dog abundance; (2) surveys of dik-dik population densities conducted before and after wild dog recovery; and (3) two separate, replicated, herbivore-exclusion experiments initiated before and after wild dog recovery. The dik-dik population declined by 33% following wild dog recovery, which is best explained by wild dog predation. Dik-dik browsing suppressed tree abundance, but the strength of suppression did not differ between before and after wild dog recovery. Despite strong, top-down limitation between adjacent trophic levels (carnivore-herbivore and herbivore-plant), a trophic cascade did not occur, possibly because of a time lag in indirect effects, variation in rainfall, and foraging by herbivores other than dik-dik. Our ability to reject the trophic-cascade hypothesis required two important approaches: (1) temporally replicated herbivore exclusions, separately established before and after wild dog recovery; and (2) evaluating multiple drivers of variation in the abundance of dik-dik and trees. While the restoration of large carnivores is often a conservation priority, our results suggest that indirect effects are mediated by ecological context, and that trophic cascades are not a foregone conclusion of such recoveries. C1 [Ford, Adam T.] Univ British Columbia, Dept Zool, Vancouver, BC V6T 1Z4, Canada. [Ford, Adam T.; Goheen, Jacob R.; Kinnaird, Margaret F.; O'Brien, Timothy G.; Palmer, Todd M.; Pringle, Robert M.; Woodroffe, Rosie] Mpala Res Ctr, Nanyuki, Kenya. [Goheen, Jacob R.] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Augustine, David J.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Kinnaird, Margaret F.; O'Brien, Timothy G.] Wildlife Conservat Soc, Global Conservat Programs, Bronx, NY 10460 USA. [Palmer, Todd M.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Pringle, Robert M.] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Woodroffe, Rosie] Zool Soc London, Inst Zool, London NW1 4RY, England. RP Ford, AT (reprint author), Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. EM adamford@uoguelph.ca FU Natural Sciences and Engineering Research Council; Killam Pre-doctoral Fellowship; American Society of Mammalogists; University of British Columbia; University of Wyoming; Mpala Research Trust; USAID; Wildlife Conservation Society; National Geographic Society; African Wildlife Foundation; Denver Zoo FX This research was supported by grants from the Natural Sciences and Engineering Research Council (A. T. Ford, J. R. Goheen), a Killam Pre-doctoral Fellowship (A. T. Ford), the American Society of Mammalogists (A. T. Ford), University of British Columbia (A. T. Ford), University of Wyoming (J. R. Goheen), Mpala Research Trust (M. F. Kinnaird), USAID (M. F. Kinnaird, T. G. O'Brien), Wildlife Conservation Society (M. F. Kinnaird, T. G. O'Brien, R. Woodroffe), National Geographic Society (R. Woodroffe), African Wildlife Foundation (R. Woodroffe), and Denver Zoo (R. Woodroffe). NR 69 TC 5 Z9 5 U1 12 U2 78 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD OCT PY 2015 VL 96 IS 10 BP 2705 EP 2714 DI 10.1890/14-2056.1.sm PG 10 WC Ecology SC Environmental Sciences & Ecology GA CT5MD UT WOS:000362853600014 PM 26649391 ER PT J AU Robert, CAM Schirmer, S Barry, J French, BW Hibbard, BE Gershenzon, J AF Robert, Christelle A. M. Schirmer, Stefanie Barry, Julie French, B. Wade Hibbard, Bruce E. Gershenzon, Jonathan TI Belowground herbivore tolerance involves delayed overcompensatory root regrowth in maize SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article; Proceedings Paper CT 15th International Symposium on Insect-Plant Relationships (SIP) CY AUG 17-22, 2014 CL Univ Neuchatel, Neuchatel, SWITZERLAND HO Univ Neuchatel DE Diabrotica virgifera; Zea mays; root feeder; root behavior; root growth timing; western corn rootworm; Poaceae; Coleoptera; Chrysomelidae ID WESTERN CORN-ROOTWORM; COLEOPTERA-CHRYSOMELIDAE; RAPID CHANGES; CARBON ALLOCATION; PLANT-RESISTANCE; OPTIMIZATION; GERMPLASM; COSTS; ESTABLISHMENT; TEMPERATURE AB Plants can tolerate leaf-herbivore attack through metabolic reconfigurations that allow for the rapid regrowth of lost leaves. Several studies indicate that root-attacked plants can re-allocate resources to the aboveground parts. However, the connection between tolerance and root regrowth remains poorly understood. We investigated the timing and extent of root regrowth of tolerant and susceptible lines of maize, Zeamays L. (Poaceae), attacked by the western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), in the laboratory and the field. Infested tolerant maize plants produced more root biomass and even overcompensated for the lost roots, whereas this effect was absent in susceptible lines. Furthermore, the tolerant plants slowed growth of new roots in the greenhouse and in the field 4-8 days after infestation, whereas susceptible plants slowed growth of new roots only in the field and only after 12 days of infestation. The quick response of tolerant lines may have enabled them to escape root attack by starving the herbivores and by saving resources for regrowth after the attack had ceased. We conclude that both timing and the extent of regrowth may determine plant tolerance to root herbivory. C1 [Robert, Christelle A. M.] Univ Bern, Biot Interact, Inst Plant Sci, CH-3013 Bern, Switzerland. [Robert, Christelle A. M.; Schirmer, Stefanie; Gershenzon, Jonathan] Max Planck Inst Chem Ecol, Dept Biochem, D-07745 Jena, Germany. [Barry, Julie; Hibbard, Bruce E.] Univ Missouri, Plant Genet Res Univ, USDA ARS, Columbia, MO 65211 USA. [French, B. Wade] USDA ARS, North Cent Agr Res Lab, Brookings, SD USA. RP Robert, CAM (reprint author), Univ Bern, Sect Biot Interact, Inst Plant Sci, Altenbergrain 21, CH-3013 Bern, Switzerland. EM christelle.robert@ips.unibe.ch RI Gershenzon, Jonathan/K-1331-2013 OI Gershenzon, Jonathan/0000-0002-1812-1551 FU Swiss National Science Foundation [PBNEP3_140196, CRSII3_136184] FX We thank Matthias Erb, Matt Higdon, Chad Nielson, and the whole field crew for their kind contribution to field experiments, and Maxime Herve for statistical support. We are also grateful to the whole Root Herbivore Interactions Group members (MPI-CE) for their help in transplanting the maize plants in the greenhouse. Research activity by CAMR was supported by the Swiss National Science Foundation (PBNEP3_140196). This study was partially supported by a Sinergia grant from the Swiss National Science Foundation (CRSII3_136184). NR 47 TC 4 Z9 4 U1 2 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD OCT PY 2015 VL 157 IS 1 SI SI BP 113 EP 120 DI 10.1111/eea.12346 PG 8 WC Entomology SC Entomology GA CT7IV UT WOS:000362989300012 ER PT J AU Kemp, EA Cottrell, TE AF Kemp, E. A. Cottrell, T. E. TI Effect of Lures and Colors on Capture of Lady Beetles (Coleoptera: Coccinellidae) in Tedders Pyramidal Traps SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Harmonia axyridis; Tedders trap; lure; methyl salicylate; limonene ID HARMONIA-AXYRIDIS COLEOPTERA; COLEOMEGILLA-MACULATA; CHRYSOPERLA-CARNEA; METHYL SALICYLATE; SAMPLING METHODS; OLFACTORY CUES; PLUM CURCULIO; STICKY CARDS; HEMIPTERA; PENTATOMIDAE AB Purposeful attraction and aggregation of adult Coccinellidae at target sites would be useful for sampling purposes and pest suppression. We field-tested 1) lures in yellow and black pyramidal traps and 2) pyramidal traps that had been painted one or two colors (without lures) to determine if lures or trap color affected capture of adult Coccinellidae. In only one experiment with lures did a single rate of limonene increase trap capture, whereas no other lure ever did. Yellow traps, regardless of using a lure, always captured significantly more lady beetles than black traps. When single-color red, orange, yellow, green, blue, purple, black, and white traps (without lures) were tested, yellow traps captured significantly more lady beetles. Of all species of Coccinellidae captured in these single-color traps, 95% were the exotic species Harmonia axyridis (Pallas) and Coccinella septempunctata L. H. axyridis alone dominated trap capture comprising 74.1% of all lady beetles. Two-color traps (yellow-green, yellow-orange, yellow-white, and yellow-black) never captured more than single-color yellow traps. These results demonstrate that yellow pyramidal traps can be used to purposefully attract, and when used without a collection device, possibly aggregate adult Coccinellidae at targeted field sites. C1 [Kemp, E. A.; Cottrell, T. E.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Cottrell, TE (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM Ted.Cottrell@ars.usda.gov NR 49 TC 1 Z9 1 U1 5 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2015 VL 44 IS 5 BP 1395 EP 1406 DI 10.1093/ee/nvv108 PG 12 WC Entomology SC Entomology GA CT5BF UT WOS:000362820900012 PM 26314010 ER PT J AU Machtinger, ET Geden, CJ AF Machtinger, E. T. Geden, C. J. TI Comparison of the Olfactory Preferences of Four of Filth Fly Pupal Parasitoid Species (Hymenoptera: Pteromalidae) for Hosts in Equine and Bovine Manure SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE olfactometer; Musca domestica; Stomoxys calcitrans; Spalangia; Muscidifurax ID MUSCIDIFURAX-RAPTOR HYMENOPTERA; HOUSE-FLIES DIPTERA; SPALANGIA-CAMERONI HYMENOPTERA; MUSCA-DOMESTICA CONTROL; STABLE FLY; SUSTAINED RELEASES; MUSCIDAE PUPAE; POULTRY HOUSES; ZARAPTOR HYMENOPTERA; INTRAGUILD PREDATION AB House flies (Musca domestica L.) and stable flies (Stomoxys calcitrans (L.)) (Diptera: Muscidae) are common pests in equine and cattle facilities. Pupal parasitoids, primarily in the genera Spalangia and Muscidifurax (Hymenoptera: Pteromalidae), can be purchased for biological control of these flies. However, little is known about the host-habitat preferences associated with host-seeking by these parasitoids. The preferences of two Spalangia and two Muscidifurax species to odors associated with house fly hosts in equine and bovine manure were investigated in the laboratory using a Y-tube olfactometer. Odor stimuli from manure without developing flies, third-instar house flies in manure, and fly host puparia in manure were evaluated. In choice tests, S. cameroni and S. endius were strongly attracted to odor associated with equine manure against clean air. Although S. cameroni was attracted to all bovine manure-containing treatments against clean air, S. endius was only attracted to the bovine manure with third-instar flies. There were no significant differences between the Spalangia species in odor responses. Neither Muscidifurax species were attracted to equine manure treatments and were only attracted to the bovine manure with puparia over clean air. In manure comparison studies, bovine treatments with developing flies were more attractive than the equivalent equine treatments to both Muscidifurax species The data suggest that coexistence between the competing pteromalid parasitoids might be promoted by different host-seeking behaviors. Additionally, manure preferences may indicate parasitoid suitability for releases on different livestock and equine facilities. C1 [Machtinger, E. T.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Geden, C. J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Machtinger, ET (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. EM irishtangerine@ufl.edu FU United States Department of Agriculture Southern Sustainable Agriculture and Research Education (SARE) award [GS-11-101] FX We would like to thank Caitlin Taylor for her extensive help conducting the bioassays and Dana Johnson for maintaining laboratory fly colonies. The reviews of this manuscript by Dr. Heather McAuslane and Dr. Hans Alborn are appreciated, as is the help of Rudy Strohschein on his design of the glassware used in the experiments. We would like to thank Dr. Peter Teal for inspiring this continued work with parasitoid chemical ecology. This research was supported by a United States Department of Agriculture Southern Sustainable Agriculture and Research Education (SARE) award #GS-11-101. NR 51 TC 1 Z9 1 U1 6 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2015 VL 44 IS 5 BP 1417 EP 1424 DI 10.1093/ee/nvv120 PG 8 WC Entomology SC Entomology GA CT5BF UT WOS:000362820900014 PM 26314022 ER PT J AU Knight, AL Basoalto, E Katalin, J El-Sayed, AM AF Knight, A. L. Basoalto, E. Katalin, J. El-Sayed, A. M. TI A Binary Host Plant Volatile Lure Combined With Acetic Acid to Monitor Codling Moth (Lepidoptera: Tortricidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Monitoring; apple; pear ester; nonatriene ID CYDIA-POMONELLA LEPIDOPTERA; PHEROMONE-TREATED ORCHARDS; PEAR ESTER; SEX-PHEROMONE; APPLE ORCHARDS; MATING DISRUPTION; TREE VOLATILES; ETHYL (E; CODLEMONE; MANAGEMENT AB Field studies were conducted in the United States, Hungary, and New Zealand to evaluate the effectiveness of septa lures loaded with ethyl (E,Z)-2,4-decadienoate (pear ester) and (E)-4,8-dimethyl-1,3,7-nonatriene (nonatriene) alone and in combination with an acetic acid co-lure for both sexes of codling moth, Cydia pomonella (L.). Additional studies were conducted to evaluate these host plant volatiles and acetic acid in combination with the sex pheromone, (E,E)-8,10-dodecadien-1-ol (codlemone). Traps baited with pear ester/nonatriene + acetic acid placed within orchards treated either with codlemone dispensers or left untreated caught significantly more males, females, and total moths than similar traps baited with pear ester + acetic acid in some assays. Similarly, traps baited with codlemone/pear ester/nonatriene + acetic acid caught significantly greater numbers of moths than traps with codlemone/pear ester + acetic acid lures in some assays in orchards treated with combinational dispensers (dispensers loaded with codlemone/pear ester). These data suggest that monitoring of codling moth can be marginally improved in orchards under variable management plans using a binary host plant volatile lure in combination with codlemone and acetic acid. These results are likely to be most significant in orchards treated with combinational dispensers. Significant increases in the catch of female codling moths in traps with the binary host plant volatile blend plus acetic acid should be useful in developing more effective mass trapping strategies. C1 [Knight, A. L.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Basoalto, E.] Univ Austral Chile, Inst Prod & Sanidad Vegetal, Fac Ciencias Agr, Valdivia, Chile. [Katalin, J.] Inst Plant Protect, H-1525 Budapest, Hungary. [El-Sayed, A. M.] Agr & Sci Ctr, NZ Inst Plant & Food Res, Lincoln, New Zealand. RP Knight, AL (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov RI Basoalto Venegas, Esteban/A-6384-2016 OI Basoalto Venegas, Esteban/0000-0003-2280-8978 FU OTKA grant of HAS [K81494] FX We would like to thank Bill Lingren, Trece Inc., Adair, OK, for providing materials. Duane Larsen (ARS, U.S.D.A., Wapato, WA) assisted in the U.S. field trials. Research conducted in Hungary was partially supported by OTKA grant K81494 of HAS. We thank Laszlo Weinelt (Tordas) and Tamas Vikor (Soltvadkert) for providing the experimental site in Hungary. NR 37 TC 1 Z9 1 U1 3 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2015 VL 44 IS 5 BP 1434 EP 1440 DI 10.1093/ee/nvv116 PG 7 WC Entomology SC Entomology GA CT5BF UT WOS:000362820900016 PM 26314018 ER PT J AU Landolt, PJ Cha, DH Zack, RS AF Landolt, Peter J. Cha, Dong H. Zack, Richard S. TI Synergistic Trap Response of the False Stable Fly and Little House Fly (Diptera: Muscidae) to Acetic Acid and Ethanol, Two Principal Sugar Fermentation Volatiles SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Muscidae; fly; attractant; lure; trap; synergy ID MUSCA-DOMESTICA DIPTERA; XESTIA-C-NIGRUM; BAITED JUG-TRAP; CHEMICAL ATTRACTANTS; SYNANTHROPIC FLIES; DROSOPHILA-SUZUKII; MAMESTRA-CONFIGURATA; SOUTHERN CALIFORNIA; LACANOBIA-SUBJUNCTA; FANNIA-CANICULARIS AB In an initial observation, large numbers of muscoid flies (Diptera) were captured as nontarget insects in traps baited with solutions of acetic acid plus ethanol. In subsequent field experiments, numbers of false stable fly Muscina stabulans (Fallen) and little house fly Fannia canicularis (L.) trapped with the combination of acetic acid plus ethanol were significantly higher than those trapped with either chemical alone, or in unbaited traps. Flies were trapped with acetic acid and ethanol that had been formulated in the water of the drowning solution of the trap, or dispensed from polypropylene vials with holes in the vial lids for diffusion of evaporated chemical. Numbers of both species of fly captured were greater with acetic acid and ethanol in glass McPhail traps, compared to four other similar wet trap designs. This combination of chemicals may be useful as an inexpensive and not unpleasant lure for monitoring or removing these two pest fly species. C1 [Landolt, Peter J.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Zack, Richard S.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. RP Landolt, PJ (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov FU Washington State Tree Fruit Research Commission FX Technical assistance was provided by J. Alfaro, J. Beauchene, J. Brumley, P. Chapman, D. Green, D. Larson, and C. Smithhisler. This work was supported in part by funding from the Washington State Tree Fruit Research Commission. NR 47 TC 2 Z9 2 U1 4 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2015 VL 44 IS 5 BP 1441 EP 1448 DI 10.1093/ee/nvv119 PG 8 WC Entomology SC Entomology GA CT5BF UT WOS:000362820900017 PM 26314021 ER PT J AU McHugh, T Hoff, R AF McHugh, Tara Hoff, Ron TI All Sorts of Options for Food Product Sorting SO FOOD TECHNOLOGY LA English DT Editorial Material C1 [McHugh, Tara] USDA ARS, Albany, CA 94710 USA. [Hoff, Ron] ARS, Hlth Proc Foods Res Unit, Albany, CA USA. RP McHugh, T (reprint author), USDA ARS, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov; ron.haff@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD OCT PY 2015 VL 69 IS 10 BP 74 EP 76 PG 3 WC Food Science & Technology SC Food Science & Technology GA CT6LE UT WOS:000362923700015 ER PT J AU Bradford, SA Torkzaban, S Leij, F Simunek, J AF Bradford, Scott A. Torkzaban, Saeed Leij, Feike Simunek, Jiri TI Equilibrium and kinetic models for colloid release under transient solution chemistry conditions SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Colloid; Microorganism; Model; Transients; Chemistry ID SATURATED POROUS-MEDIA; CHEMICAL CONDITIONS; IONIC-STRENGTH; CATION-EXCHANGE; PRIMARY-MINIMUM; FLOW-RATE; TRANSPORT; PARTICLES; ADSORPTION; SURFACES AB We present continuum models to describe colloid release in the subsurface during transient physicochemical conditions. Our modeling approach relates the amount of colloid release to changes in the fraction of the solid surface area that contributes to retention. Equilibrium, kinetic, equilibrium and kinetic, and two-site kinetic models were developed to describe various rates of colloid release. These models were subsequently applied to experimental colloid release datasets to investigate the influence of variations in ionic strength (IS), pH, cation exchange, colloid size, and water velocity on release. Various combinations of equilibrium and/or kinetic release models were needed to describe the experimental data depending on the transient conditions and colloid type. Release of Escherichia coli D21g was promoted by a decrease in solution IS and an increase in pH, similar to expected trends for a reduction in the secondary minimum and nanoscale chemical heterogeneity. The retention and release of 20 nm carboxyl modified latex nanopardcles (NPs) were demonstrated to be more sensitive to the presence of Ca2+ than D21g. Specifically, retention of NPs was greater than D21g in the presence of 2 mM CaCl2 solution, and release of NPs only occurred after exchange of Ca2+ by Na+ and then a reduction in the solution IS. These findings highlight the limitations of conventional interaction energy calculations to describe colloid retention and release, and point to the need to consider other interactions (e.g., Born, steric, and/ or hydration forces) and/or nanoscale heterogeneity. Temporal changes in the water velocity did not have a large influence on the release of D21g for the examined conditions. This insensitivity was likely due to factors that reduce the applied hydrodynamic torque and/or increase the resisting adhesive torque; e.g., macroscopic roughness and grain-grain contacts. Our analysis and models improve our understanding and ability to describe the amounts and rates of colloid release and indicate that episodic colloid transport is expected under transient physicochemical conditions. Published by Elsevier B.V. C1 [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. [Torkzaban, Saeed] CSIRO Land & Water, Glen Osmond, SA 5064, Australia. [Leij, Feike] Calif State Univ Long Beach, Dept Civil Engn & Construct Engn Management, Long Beach, CA 90840 USA. [Simunek, Jiri] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. RP Bradford, SA (reprint author), ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. EM Scott.Bradford@ars.usda.gov RI Torkzaban, Saeed/G-7377-2013 OI Torkzaban, Saeed/0000-0002-5146-9461 FU USDA-ARS FX This research was supported by the 214 Manure and Byproduct Utilization Project of the USDA-ARS. Mention of trade names and company names in this manuscript does not imply any endorsement or preferential treatment by the USDA. We would also like to acknowledge the help of Dr. Brendan Headd in conducting these transport and release experiments. NR 55 TC 8 Z9 8 U1 12 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 EI 1873-6009 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD OCT PY 2015 VL 181 SI SI BP 141 EP 152 DI 10.1016/j.jconhyd.2015.04.003 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA CT6JF UT WOS:000362918600012 PM 25913320 ER PT J AU Torkzaban, S Bradford, SA Vanderzalm, JL Patterson, BM Harris, B Prommer, H AF Torkzaban, Saeed Bradford, Scott A. Vanderzalm, Joanne L. Patterson, Bradley M. Harris, Brett Prommer, Henning TI Colloid release and clogging in porous media: Effects of solution ionic strength and flow velocity SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Release; In-situ colloids; Clogging; Permeability; Modeling ID SOLUTION CHEMISTRY; CHEMICAL CONDITIONS; PARTICLE RELEASE; AQUIFER STORAGE; TRANSPORT; WATER; PERMEABILITY; DEPOSITION; IMMOBILIZATION; HYDRODYNAMICS AB The release and retention of in-situ colloids in aquifers play an important role in the sustainable operation of managed aquifer recharge (MAR) schemes. The processes of colloid release, retention, and associated permeability changes in consolidated aquifer sediments were studied by displacing native groundwater with reverse osmosis-treated (RO) water at various flow velocities. Significant amounts of colloid release occurred when: (i) the native groundwater was displaced by RO-water with a low ionic strength (IS), and (ii) the flow velocity was increased in a stepwise manner. The amount of colloid release and associated permeability reduction upon RO-water injection depended on the initial clay content of the core. The concentration of released colloids was relatively low and the permeability reduction was negligible for the core sample with a low clay content of about 1.3%. In contrast, core samples with about 6 and 7.5% clay content exhibited: (i) close to two orders of magnitude increase in effluent colloid concentration and (ii) more than 65% permeability reduction. Incremental improvement in the core permeability was achieved when the flow velocity increased, whereas a short flow interruption provided a considerable increase in the core permeability. This dependence of colloid release and permeability changes on flow velocity and colloid concentration was consistent with colloid retention and release at pore constrictions due to the mechanism of hydrodynamic bridging. A mathematical model was formulated to describe the processes of colloid release, transport, retention at pore constrictions, and subsequent permeability changes. Our experimental and modeling results indicated that only a small fraction of the in-situ colloids was released for any given change in the IS or flow velocity. Comparison of the fitted and experimentally measured effluent colloid concentrations and associated changes in the core permeability showed good agreement, indicating that the essential physics were accurately captured by the model. (C) 2015 Elsevier B.V. All rights reserved. C1 [Torkzaban, Saeed; Vanderzalm, Joanne L.] CSIRO Land & Water, Glen Osmond, SA 5064, Australia. [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA USA. [Patterson, Bradley M.; Prommer, Henning] CSIRO Land & Water, Floreat Pk, WA, Australia. [Patterson, Bradley M.] Univ Western Australia, Sch Chem & Biochem, Crawley 6009, Australia. [Harris, Brett] Curtin Univ, Dept Explorat Geophys, Perth, WA 6151, Australia. [Prommer, Henning] Univ Western Australia, Sch Earth & Environm, Crawley 6009, Australia. [Prommer, Henning] Flinders Univ S Australia, Natl Ctr Groundwater Res & Training, Adelaide, SA 5001, Australia. RP Torkzaban, S (reprint author), CSIRO Land & Water, Glen Osmond, SA 5064, Australia. EM Saeed.Torkzaban@csiro.au RI Prommer, Henning/A-4555-2008; Torkzaban, Saeed/G-7377-2013; Patterson, Bradley/B-8309-2009; Vanderzalm, Joanne/F-2339-2011 OI Prommer, Henning/0000-0002-8669-8184; Torkzaban, Saeed/0000-0002-5146-9461; FU Water Corporation of Western Australia; Gas Industry Social and Environmental Research Alliance (GISERA) FX This work was supported by the Water Corporation of Western Australia and the Gas Industry Social and Environmental Research Alliance (GISERA). GISERA is a collaborative vehicle established by CSIRO and Australia Pacific LNG to undertake publicly reported research addressing the socioeconomic and environmental impacts of Australia's natural gas industries. For more details about GISERA visit www.gisera.org.au. All data can be provided upon request from the corresponding author. NR 57 TC 8 Z9 9 U1 5 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 EI 1873-6009 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD OCT PY 2015 VL 181 SI SI BP 161 EP 171 DI 10.1016/j.jconhyd.2015.06.005 PG 11 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA CT6JF UT WOS:000362918600014 PM 26141344 ER PT J AU Chen, HL Liu, LP Wang, LX Wang, SH Wang, ML Cheng, XZ AF Chen, Honglin Liu, Liping Wang, Lixia Wang, Suhua Wang, Ming Li Cheng, Xuzhen TI Development of SSR markers and assessment of genetic diversity of adzuki bean in the Chinese germplasm collection SO MOLECULAR BREEDING LA English DT Article DE Vigna angularis; Simple sequence repeat (SSR) marker; Expressed sequence tag (EST)-SSR marker; Genetic diversity; Germplasm utilization ID ANGULARIS WILLD. OHWI; COWPEA VIGNA-UNGUICULATA; RADIATA L. WILCZEK; POLYMERASE-CHAIN-REACTION; PEA PISUM-SATIVUM; POPULATION-STRUCTURE; EUKARYOTIC GENOMES; LINKAGE MAP; TRANSFERABILITY; OHASHI AB Adzuki bean is an important food legume crop in East Asia. China is ranked as the number one country for its production and consumption. A large number of adzuki bean accessions are maintained in the Chinese national seed genebank. Tapping its genetic diversity may have potential in assisting breeding programs for new cultivar development and improvement. However, its genetic diversity was not well evaluated due to lack of sufficient DNA markers. For marker development, we initiated de novo transcriptome sequencing of adzuki bean. From sequence assembly, 65,950 unigenes were identified, and some of them were used for EST-SSR (eSSR) marker development. A set of 53 SSR markers were developed from adzuki bean, and 110 SSR (including 57 already available) markers were used for genotyping of 261 selected accessions. These adzuki bean accessions were classified into 10 clusters. The accessions from North China were well separated from the accessions from South China. The average polymorphic information content of genomic SSR (gSSR) markers and eSSR markers was 0.5081 and 0.2382, respectively. Results indicate that gSSR markers were more polymorphic than eSSR markers. These polymorphic eSSR and gSSR markers will be useful for assisting adzuki-bean-breeding programs and future germplasm curation. C1 [Chen, Honglin; Wang, Lixia; Wang, Suhua; Cheng, Xuzhen] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China. [Liu, Liping] China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China. [Wang, Ming Li] ARS, USDA, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. RP Cheng, XZ (reprint author), Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China. EM chengxuzhen@caas.cn FU Agricultural Science and Technology Innovation Program (ASTIP) in CAAS; China Agriculture Research System from Ministry of Agriculture of China [CARS-09] FX This study was funded by the Agricultural Science and Technology Innovation Program (ASTIP) in CAAS and the China Agriculture Research System (CARS-09) from the Ministry of Agriculture of China. NR 43 TC 2 Z9 2 U1 4 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD OCT PY 2015 VL 35 IS 10 AR 191 DI 10.1007/s11032-015-0383-5 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CT6WD UT WOS:000362952600004 ER PT J AU Qi, LL Long, YM Ma, GJ Markell, SG AF Qi, L. L. Long, Y. M. Ma, G. J. Markell, S. G. TI Map saturation and SNP marker development for the rust resistance genes (R-4, R-5, R-13a, and R-13b) in sunflower (Helianthus annuus L.) SO MOLECULAR BREEDING LA English DT Article DE Sunflower rust; Resistance genes; Sunflower; SNP markers; Gene pyramiding ID SCLEROTINIA MIDSTALK-ROT; ASSISTED SELECTION; PUCCINIA-HELIANTHI; CULTIVATED SUNFLOWER; DOWNY MILDEW; SUPPRESSED RECOMBINATION; KARYOTYPIC EVOLUTION; NORTH-DAKOTA; LINKAGE MAPS; RICH REGIONS AB Sunflower rust, which is incited by the fungus Puccinia helianthi Schwein., is the most common disease in Australia, Argentina, South Africa, and North America. Three independent genes, R-5, R-4, and R-13 with two alleles R-13a and R-13b, were discovered in sunflower and are promising sources of resistance to rust. R-5 was previously mapped to linkage group (LG) 2, and R-4 and R-13 were mapped to LG13 of the sunflower genome using simple sequence repeat (SSR) markers. The objective of this study was to finely map R-5, R-4, R-13a, and R-13b using newly developed single nucleotide polymorphism (SNP) markers in four F-2 populations previously used for SSR mapping. Of the 67 LG2 SNP markers screened, two SNPs, SFW03654 and NSA_000267, flanked R-5 at a genetic distance of 0.6 and 1.2 cM, respectively. This flanking narrowed the genetic interval containing R-5 from 5.1 to 1.8 cM in length. A total of 69 LG13 SNP markers were analyzed in the R-4, R-13a, and R-13b populations. In the R-4 consensus map, the gene R-4 was flanked by seven SNP loci; three co-segregating SNPs are on one side (0.7 cM proximal) and four on the other side (0.6 cM distal). Similarly, SNP markers that are tightly linked to both R-13a and R-13b were identified. R-13a was flanked by SNP markers at genetic distances of 0.4 and 0.2 cM. The SNP SFW00757 co-segregated with R-13b, and another three co-segregating SNPs were 2.4 cM proximal to R-13b. A total of 368 F-2 plants from the cross between a resistant BC3F2 plant carrying R-5 with HA-R6 carrying R-13a were first screened by SSR markers to identify "double-resistant'' lines. Twelve F-2 plants were identified to be homozygous for a combination of R-5 and R-13a, which was further confirmed with additional SNP markers developed in the present study. The double- resistant line developed in this study may provide a source of durable resistance to manage rust in confection sunflower production and will help mitigate selection pressure on each gene by the pathogen. C1 [Qi, L. L.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Long, Y. M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Ma, G. J.; Markell, S. G.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. RP Qi, LL (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU USDA-AMS Specialty Crop Block Grant Program [12-25-B-1480]; USDA-ARS CRIS [5442-21000-039-00D] FX The authors would like to thank Dr. Loren Rieseberg for providing access to the Sunflower Genome Data Repository. We also thank Drs. Xiwen Cai and Hongxia Wang for critical review of the manuscript, and Angelia Hogness for technical assistance. This project was supported by the USDA-AMS Specialty Crop Block Grant Program 12-25-B-1480, and the USDA-ARS CRIS Project No. 5442-21000-039-00D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 89 TC 1 Z9 1 U1 3 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD OCT PY 2015 VL 35 IS 10 AR 196 DI 10.1007/s11032-015-0380-8 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CT6WD UT WOS:000362952600009 ER PT J AU Dubey, JP Hilali, M Van Wilpe, E Calero-Bernal, R Verma, SK Abbas, IE AF Dubey, J. P. Hilali, M. Van Wilpe, E. Calero-Bernal, R. Verma, S. K. Abbas, I. E. TI A review of sarcocystosis in camels and redescription of Sarcocystis cameli and Sarcocystis ippeni sarcocysts from the one-humped camel (Camelus dromedarius) SO PARASITOLOGY LA English DT Article DE Sarcocystis cameli; Sarcocystis ippeni; one-humped camel (Camelus dromedarius); electron microscopy; ultrastructure ID SAUDI-ARABIA; PREVALENCE; MEAT; IRAN; DOG; NOMENCLATURE; ANIMALS; MASON; SHEEP AB There is considerable confusion concerning Sarcocystis species in camels. Five species: Sarcocystis cameli, Sarcocystis ippeni, Sarcocystis camelicanis, Sarcocystis camelocanis and Sarcocystis miescheri were named with inadequate descriptions and no type specimens. Here, we review literature on sarcocystosis in camels worldwide and redescribe structure of S. cameli and S. ippeni sarcocysts by light- and transmission electron microscopy (LM and TEM). Eight sarcocysts from the oesophagi of two camels (Camelus dromedarius) from Egypt were studied. By LM, all sarcocysts were thin-walled with barely visible projections on the cyst walls. By TEM, two structurally distinct sarcocysts were recognized by unique villar protrusions (vp) not found in sarcocysts from any other host. Sarcocysts of S. cameli had vp of type 9j. The sarcocyst wall had upright slender vp, up to 3.0 mu m long and 0.5 mu m wide; the total thickness of the sarcocyst wall with ground substance (gs) layer was 3.5 mu m. On each vp, there were rows of knob-like protrusions that appeared to be interconnected. The vp had microtubules that originated at midpoint of the gs and continued up to the tip; microtubules were smooth, without any granules or dense areas. Bradyzoites were approximately 14-15 x 3-4 mu m in size with typical organelles. Sarcocystis ippeni sarcocysts had type 32 sarcocyst wall characterized by conical vp with an electron dense knob. The total thickness of the sarcocyst wall (from the base of gs to vp tip) was 2.3-3.0 mu m. The vp were up to 1.2 mu m wide at the base and 0.25 mu m at the tip. Microtubules in vp originated at midpoint of gs and continued up to tip; microtubules were criss-crossed, smooth and without granules or dense areas. Bradyzoites were 12.0-13.5 x 2.0-3.0 mu m in size. Sarcocystis camelicanis, S. camelocanis and S. miescheri are considered invalid. C1 [Dubey, J. P.; Calero-Bernal, R.; Verma, S. K.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Hilali, M.] Cairo Univ, Dept Parasitol, Fac Vet Med, Giza 12211, Egypt. [Van Wilpe, E.] Univ Pretoria, Dept Anat & Physiol, Fac Vet Sci, ZA-0110 Onderstepoort, South Africa. [Abbas, I. E.] Fac Vet Med, Dept Parasitol, Mansoura, Egypt. RP Dubey, JP (reprint author), ARS, USDA, APDL, BARC East, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov RI Hilali, Mosaad/O-9443-2016; OI A. Hilali, Mosaad/0000-0002-9930-8501 FU Department of Employment and Innovation of the Regional Government of Extremadura (Spain) [PO12010]; European Social Fund FX R. Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund. NR 35 TC 1 Z9 1 U1 1 U2 3 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD OCT PY 2015 VL 142 IS 12 BP 1481 EP 1492 DI 10.1017/S0031182015000852 PG 12 WC Parasitology SC Parasitology GA CT6GK UT WOS:000362911100003 PM 26416197 ER PT J AU Fontenot, KR Nguyen, MM Al-Abdul-Wahid, MS Easson, MW Chang, SC Lorigan, GA Condon, BD AF Fontenot, Krystal R. Nguyen, Monique M. Al-Abdul-Wahid, M. Sameer Easson, Michael W. Chang, SeChin Lorigan, Gary A. Condon, Brian D. TI The thermal degradation pathway studies of a phosphazene derivative on cotton fabric SO POLYMER DEGRADATION AND STABILITY LA English DT Article DE Phosphazene derivative; ATR-IR; TGA-FTIR; Solid-state NMR; Thermal degradation pathway ID PHOSPHORUS-NITROGEN COMPOUNDS; SOLID-STATE NMR; FLAME-RETARDANT; PHOSPHONITRILIC COMPOUNDS; EPOXY-RESIN; SIDE-GROUPS; CELLULOSE; POLYMERIZATION; DECOMPOSITION; POLYMERS AB Phosphazene derivatives have been recognized as promising flame retardants for numerous synthetic polymeric systems. However, limited studies are available for phosphazene derivatives on natural polymeric systems such as cotton fabric. The flammability and thermal stability of fabric treated with a phosphazene derivative 1,1,3,3-dihydroxybipheny1-5,5-diaminoethanephosphazene (dBEP) indicated that only 9 wt% add-on of dBEP was required to achieve promising flame retardant properties on cotton fabric. To understand the mode of action of dBEP, the thermal degradation pathways of the control and cotton fabric treated with dBEP were investigated. Thermogravimetric analysis coupled with Fourier transform infrared spectroscopy (TGA-FTIR) was used to follow the evolved gases produced by the control and treated fabrics during thermal degradation. Two techniques, attenuated total reflectance infrared spectroscopy (ATR-IR) and solid-state nuclear magnetic resonance (NMR) were employed to examine the degraded residues of the unburned fabrics, burned fabrics, and dBEP. The results show that dBEP undergoes decomposition to produce phosphoric acid and polymerization to form phospham-like derivative that are known to retard fire. Published by Elsevier Ltd. C1 [Fontenot, Krystal R.; Nguyen, Monique M.; Easson, Michael W.; Chang, SeChin; Condon, Brian D.] USDA, Cotton Chem & Utilizat Res, New Orleans, LA 70124 USA. [Al-Abdul-Wahid, M. Sameer; Lorigan, Gary A.] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. RP Easson, MW (reprint author), USDA, Cotton Chem & Utilizat Res, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Michael.Easson@ars.usda.gov FU U. S. Department of Agriculture [6054-41430-007-00] FX This project was financed by the U. S. Department of Agriculture, Project Number: 6054-41430-007-00. We thank Dr. Marsha Cole for editing the manuscript and we thank Jade Smith for her assistance with the Wiley mill. NR 75 TC 4 Z9 5 U1 7 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0141-3910 EI 1873-2321 J9 POLYM DEGRAD STABIL JI Polym. Degrad. Stabil. PD OCT PY 2015 VL 120 BP 32 EP 41 DI 10.1016/j.polymdegradstab.2015.04.032 PG 10 WC Polymer Science SC Polymer Science GA CT6MJ UT WOS:000362926800005 ER PT J AU Bajwa, DS Bajwa, SG Holt, GA AF Bajwa, Dilpreet S. Bajwa, Sreekala G. Holt, Greg A. TI Impact of biofibers and coupling agents on the weathering characteristics of composites SO POLYMER DEGRADATION AND STABILITY LA English DT Article DE Natural fiber composites; Coupling agents; Natural weathering; Polymer degradation; Mechanical properties ID WOOD-PLASTIC COMPOSITES; MECHANICAL-PROPERTIES; THERMOPLASTIC COMPOSITES; POLYPROPYLENE COMPOSITES; PERFORMANCE AB This paper explores the ultraviolet (UV) weathering performance of high density polyethylene (HDPE) composites with different biofiber fillers and coupling agent. Biofiber polymer composite (BFPC) material samples were prepared using oak, cotton burr and stem (CBS) or guayule bagasse as fiber source HDPE as resin, and two different coupling agents. Weathering variables included exposure to UV radiation and moisture cycles for up to 2200 h. Each variable can degrade BFPC matrix independently or synergistically. The impact of weathering was measured through the changes in the surface matrix, mechanical properties and thermal stability of BFPC as a function of biofibers types and coupling agents. The coupling agent treated composites showed color shift (Delta E) and variable surface degradation. Water absorption of the weathered samples continued to increase after 10 days of immersion. Flexural stiffness, strength and impact properties of weathered samples decreased regardless of fiber type or coupling agent. Minimal linear thermal expansion was noted in weathered composites with coupling agents. Overall coupling agent helped to retain the mechanical properties of composites after exposure to UV weathering. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Bajwa, Dilpreet S.] N Dakota State Univ, Dept Mech Engn, Fargo, ND 58105 USA. [Bajwa, Sreekala G.] N Dakota State Univ, Dept Agr & Biosyst Engn, Fargo, ND 58105 USA. [Holt, Greg A.] ARS, USDA, Cotton Prod & Proc Res Unit, Lubbock, TX USA. RP Bajwa, DS (reprint author), N Dakota State Univ, Dept Mech Engn, Fargo, ND 58105 USA. EM dilpreet.bajwa@ndsu.edu NR 38 TC 3 Z9 3 U1 4 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0141-3910 EI 1873-2321 J9 POLYM DEGRAD STABIL JI Polym. Degrad. Stabil. PD OCT PY 2015 VL 120 BP 212 EP 219 DI 10.1016/j.polymdegradstab.2015.06.015 PG 8 WC Polymer Science SC Polymer Science GA CT6MJ UT WOS:000362926800024 ER PT J AU Jaso, V Glenn, G Klamczynski, A Petrovic, ZS AF Jaso, Vladislav Glenn, Gregory Klamczynski, Artur Petrovic, Zoran S. TI Biodegradability study of polylactic acid/thermoplastic polyurethane blends SO POLYMER TESTING LA English DT Article DE Polylactic acid; Thermoplastic polyurethane; Biodegradability; Morphology AB Blends with varied ratio of polylactic acid (PLA) and thermoplastic polyurethane (TPU) were prepared by melt blending. The PIA content in blends was 20, 40, 60 and 80 wt%. Samples of pure PIA and TPU that underwent the same thermal treatment were also prepared. Biodegradation was examined by respirometry. Pure TPU started to degrade immediately due to degradation of the low molecular weight plasticizer in the polymer. Pure PTA, on the other hand, exhibited an incubation period after which degradation progressed rapidly and was almost complete after 70 days. The degradation profile of the blends can be correlated to their morphology. Samples with a co-continuous morphology initially degrade at a higher rate than the rest of the samples due to the higher exposure of the TPU phase in these blends. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Jaso, Vladislav; Petrovic, Zoran S.] Pittsburg State Univ, Kansas Polymer Res Ctr, Pittsburg, KS 66762 USA. [Glenn, Gregory; Klamczynski, Artur] ARS, USDA, PWA, WRRC BCE, Albany, CA 94710 USA. RP Jaso, V (reprint author), Pittsburg State Univ, Kansas Polymer Res Ctr, 1701 S Broadway, Pittsburg, KS 66762 USA. EM vjaso@pittstate.edu FU United States Department of Agriculture [USDA-CSREES 2010-38924-20706] FX This work was supported by a grant from the United States Department of Agriculture (USDA-CSREES 2010-38924-20706). NR 9 TC 4 Z9 4 U1 6 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0142-9418 EI 1873-2348 J9 POLYM TEST JI Polym. Test PD OCT PY 2015 VL 47 BP 1 EP 3 DI 10.1016/j.polymertesting.2015.07.011 PG 3 WC Materials Science, Characterization & Testing; Polymer Science SC Materials Science; Polymer Science GA CT5OF UT WOS:000362859000002 ER PT J AU Valpiani, N Wilde, P Rogers, B Stewart, H AF Valpiani, Natalie Wilde, Parke Rogers, Beatrice Stewart, Hayden TI Patterns of fruit and vegetable availability and price competitiveness across four seasons are different in local food outlets and supermarkets SO PUBLIC HEALTH NUTRITION LA English DT Article DE Fruits and vegetables; Prices; Farmers' markets; Season ID FRESH FRUIT; CONSUMPTION; COMMUNITY; DISEASE; ACCESS; US AB ObjectiveTo explore the effect of seasonality on fruit and vegetable availability and prices across three outlet types (farmers' markets, roadside stands and conventional supermarkets).DesignCross-sectional survey of geographically clustered supermarkets, farmers' markets and roadside stands. Enumerators recorded the availability and lowest price for eleven fruits and eighteen vegetables in each season of 2011.SettingPrice data were collected at retail outlets located in central and eastern North Carolina.SubjectsThe sample consisted of thirty-three supermarkets, thirty-four farmers' markets and twenty-three roadside stands.ResultsOutside the local harvest season, the availability of many fruits and vegetables was substantially lower at farmers' markets and roadside stands compared with supermarkets. Given sufficient availability, some items were significantly cheaper (P<005) at direct retail outlets in the peak season (e.g. cantaloupe cost 360 % less at roadside stands than supermarkets), while others were significantly more expensive (e.g. carrots cost 1379 % more at farmers' markets than supermarkets). Although small samples limited statistical power in many non-peak comparisons, these results also showed some differences by item: two-thirds of fruits were cheaper at one or both direct outlets in the spring and autumn, whereas five of eighteen vegetables cost more at direct retail year-round.ConclusionsCommonly consumed fruits and vegetables were more widely available at supermarkets in central and eastern North Carolina than at direct retail outlets, in each season. Contingent on item availability, price competitiveness of the direct retail outlets varied by fruit and vegetable. For many items, the outlets compete on price in more than one season. C1 [Valpiani, Natalie; Wilde, Parke; Rogers, Beatrice] Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA 02111 USA. [Stewart, Hayden] USDA, Econ Res Serv, Washington, DC USA. RP Valpiani, N (reprint author), Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, 150 Harrison Ave, Boston, MA 02111 USA. EM Natalie.Valpiani@gmail.com FU National Science Foundation Graduate Research Fellowship [DGF-0806676] FX Financial support: This material is based upon work supported by the National Science Foundation Graduate Research Fellowship (grant number DGF-0806676). The funder had no role in the design, analysis or writing of this article. NR 41 TC 0 Z9 0 U1 4 U2 10 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD OCT PY 2015 VL 18 IS 15 BP 2846 EP 2854 DI 10.1017/S1368980015000981 PG 9 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA CT6WI UT WOS:000362953200018 PM 25895583 ER PT J AU Kim, JE Lillehoj, HS Hong, YH Kim, GB Lee, SH Lillehoj, EP Bravo, DM AF Kim, Ji Eun Lillehoj, Hyun S. Hong, Yeong Ho Kim, Geun Bae Lee, Sung Hyen Lillehoj, Erik P. Bravo, David M. TI Dietary Capsicum and Curcuma longa oleoresins increase intestinal microbiome and necrotic enteritis in three commercial broiler breeds SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Necrotic enteritis; Gut; Microbiota; Phytonutrient; Immunity ID SEGMENTED FILAMENTOUS BACTERIA; CLOSTRIDIUM-PERFRINGENS; GROWTH-PERFORMANCE; EIMERIA INFECTION; GENE-EXPRESSION; CANDIDATUS ARTHROMITUS; POULTRY PRODUCTION; GUT MICROBIOTA; CHICKENS; COCCIDIOSIS AB Three commercial broiler breeds were fed from hatch with a diet supplemented with Capsicum and Curcuma longa oleoresins, and co-infected with Eimeria maxima and Clostridium perfringens to induce necrotic enteritis (NE). Pyrotag deep sequencing of bacterial 16S rRNA showed that gut microbiota compositions were quite distinct depending on the broiler breed type. In the absence of oleoresin diet, the number of operational taxonomic units (OTUs), was decreased in infected Cobb, and increased in Ross and Hubbard, compared with the uninfected. In the absence of oleoresin diet, all chicken breeds had a decreased Candidatus Arthromitus, while the proportion of Lactobacillus was increased in Cobb, but decreased in Hubbard and Ross. Oleoresin supplementation of infected chickens increased OTUs in Cobb and Ross, but decreased OTUs in Hubbard, compared with unsupplemented/infected controls. Oleoresin supplementation of infected Cobb and Hubbard was associated with an increased percentage of gut Lactobacillus and decreased Selenihalanaerobacter, while Ross had a decreased fraction of Lactobacillus and increased Selenihalanaerobacter, Clostridium, Calothrix, and Geitlerinema. These results suggest that dietary Capsicum/Curcuma oleoresins reduced the negative consequences of NE on body weight and intestinal lesion, in part, through alteration of the gut microbiome in 3 commercial broiler breeds. Published by Elsevier Ltd. C1 [Kim, Ji Eun; Lillehoj, Hyun S.; Lee, Sung Hyen] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Hong, Yeong Ho; Kim, Geun Bae] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. [Lee, Sung Hyen] Rural Dev Adm, Natl Acad Agr Sci, Wonju 565851, Jeollabuk Do, South Korea. [Lillehoj, Erik P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. [Bravo, David M.] InVivo ANH, F-56250 St Nolff, France. RP Lillehoj, HS (reprint author), ARS, USDA, Bldg 1040,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM JiEun.Kim@ARS.USDA.GOV; Hyun.Lillehoj@ARS.USDA.GOV; yhong@cau.ac.kr; kimgeun@cau.ac.kr; lshin@korea.kr; elillehoj@peds.umaryland.ed; David.BRAVO@pancosma.ch FU Next-Generation BioGreen 21 Program [PJ008084]; Rural Development Administration with Golden Seed Project [PJ00992502]; Ministry of Agriculture, Food and Rural Affairs (MAFRA); Ministry of Oceans and Fisheries (MOE); Rural Development Administration (RDA); Korea Forest Service (KFS), Investigation of the functional activity and the development of functional foods of Allium hookeri [PJ010490]; Rural Development Administration, Republic of Korea; National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2011-0011903]; USDA, ARS in-house CRIS project [1245-32000-097] FX The project was supported partly by the Next-Generation BioGreen 21 Program (No PJ008084), Rural Development Administration with Golden Seed Project (PJ00992502), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOE), Rural Development Administration (RDA) and Korea Forest Service (KFS), Investigation of the functional activity and the development of functional foods of Allium hookeri (PJ010490), Rural Development Administration, Republic of Korea, partly by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0011903), and partly by a USDA, ARS in-house CRIS project (#1245-32000-097). The authors thank Dr. Don Ritter (Mountaire Farms, Millsboro, DE) for broiler chickens. NR 61 TC 1 Z9 1 U1 6 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD OCT PY 2015 VL 102 BP 150 EP 158 DI 10.1016/j.rvsc.2015.07.022 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA CT6JN UT WOS:000362919400026 PM 26412535 ER PT J AU Riar, DS Tehranchian, P Norsworthy, JK Nandula, V McElroy, S Srivastava, V Chen, S Bond, JA Scott, RC AF Riar, Dilpreet S. Tehranchian, Parsa Norsworthy, Jason K. Nandula, Vijay McElroy, Scott Srivastava, Vibha Chen, Shu Bond, Jason A. Scott, Robert C. TI Acetolactate Synthase-Inhibiting, Herbicide-Resistant Rice Flatsedge (Cyperus iria): Cross-Resistance and Molecular Mechanism of Resistance SO WEED SCIENCE LA English DT Article DE ALS assay; ALS gene sequencing; cytochrome P450 monooxygenase-based resistance; herbicide-resistance mechanism; Illumina HiSeq; rice flatsedge ID ECHINOCHLOA-CRUS-GALLI; ORYZA-SATIVA; BENSULFURON; GENERATION; MANAGEMENT; RESPONSES; ARKANSAS; PROPANIL; WEEDS; TOOL AB Overuse of acetolactate synthase (ALS)-inhibiting herbicides in rice has led to the evolution of halosulfuron-resistant rice flatsedge in Arkansas and Mississippi. Resistant accessions were crossresistant to labeled field rates of ALS-inhibiting herbicides from four different families, in comparison to a susceptible (SUS) biotype. Resistance index of Arkansas and Mississippi accessions based on an R/S ratio of the lethal dose required for 50% plant mortality (LD50) to bispyribac-sodium, halosulfuron, imazamox, and penoxsulam was >= 21-fold. Control of Arkansas, Mississippi, and SUS accessions with labeled field rates of 2,4-D, bentazon, and propanil was >= 93%. An enzyme assay revealed that an R/S ratio for 50% inhibition (I50) of ALS for halosulfuron was 2,600 and 200 in Arkansas and Mississippi, respectively. Malathion studies did not reveal enhanced herbicide metabolism in resistant plants. The ALS enzyme assay and cross-resistance studies point toward altered a target site as the potential mechanism of resistance. Trp574-Leu amino acid substitution within the ALS gene was found in both Arkansas and Mississippi rice flatsedge accessions using the Illumina HiSeq platform, which corresponds to the mechanism of resistance found in many weed species. Field-rate applications of 2,4-D, bentazon, and propanil can be used to control these ALSresistant rice flatsedge accessions. Nomenclature: 2,4-D; acetolactate synthase; bentazon; bispyribac-sodium; halosulfuron; imazamox; malathion; propanil; rice flatsedge, Cyperus iria L; rice, Oryza sativa L. C1 [Riar, Dilpreet S.] Dow AgroSci, Indianapolis, IN 46268 USA. [Tehranchian, Parsa; Norsworthy, Jason K.; Srivastava, Vibha] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Nandula, Vijay] ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS USA. [McElroy, Scott; Chen, Shu] Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. [Bond, Jason A.] Delta Res & Extens Ctr, Stoneville, MS 38776 USA. [Scott, Robert C.] Univ Arkansas, Dept Crop Soil & Environm Sci, Extens Weed Sci, Lonoke, AR 72086 USA. RP Riar, DS (reprint author), Dow AgroSci, 9330 Zionsville Rd, Indianapolis, IN 46268 USA. EM dsriar@dow.com FU Arkansas Rice Research and Promotion Board; U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) [6402-21000-050-00D] FX Authors are thankful to the Arkansas Rice Research and Promotion Board for funding this research. A part of this work was supported by the U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) project number 6402-21000-050-00D. NR 39 TC 3 Z9 3 U1 3 U2 21 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD OCT-DEC PY 2015 VL 63 IS 4 BP 748 EP 757 DI 10.1614/WS-D-15-00014.1 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CT6XQ UT WOS:000362957200002 ER PT J AU Berger, ST Ferrell, JA Rowland, DL Webster, TM AF Berger, Sarah T. Ferrell, Jason A. Rowland, Diane L. Webster, Theodore M. TI Palmer Amaranth (Amaranthus palmeri) Competition for Water in Cotton SO WEED SCIENCE LA English DT Article DE Water competition ID GOSSYPIUM-HIRSUTUM; WEED COMPETITION; GROWTH; TRANSPIRATION; YIELD; LEAF; SOIL AB Palmer amaranth is a troublesome weed in cotton production. Yield losses of 65% have been reported from season-long Palmer amaranth competition with cotton. To determine whether water is a factor in this system, experiments were conduced in 2011, 2012, and 2013 in Citra, FL, and in Tifton, GA. In 2011, infrequent rainfall lead to drought stress. The presence of Palmer amaranth resulted in decreased soil relative water content up to 1 m in depth. Cotton stomatal conductance (g(s)) was reduced up to 1.8 m from a Palmer amaranth plant. In 2012 and 2013 higher than average rainfall resulted in excess water throughout the growing season. In this situation, no differences were found in soil relative water content or cotton g(s) as a function of proximity to Palmer amaranth. A positive linear trend was found in cotton photosynthesis and yield; each parameter increased as distance from Palmer amaranth increased. Even in these well-watered conditions, daily water use of Palmer amaranth was considerably higher than that of cotton, at 1.2 and 0.49 g H(2)0 cm(-2) d(-1), respectively. Although Palmer amaranth removed more water from the soil profile, rainfall was adequate to replenish the profile in 2 of the 3 yr of this study. However, yield loss due to Palmer amaranth was still observed despite no change in g(s), indicating other factors, such as competition for light or response to neighboring plants during development, are driving yield loss. Nomenclature: Palmer amaranth, Amaranthus palmeri S. Wats.; cotton, Gossypium hirsutum L. C1 [Berger, Sarah T.; Ferrell, Jason A.; Rowland, Diane L.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Webster, Theodore M.] USDA ARS, Tifton, GA 31793 USA. RP Ferrell, JA (reprint author), Univ Florida, Dept Agron, Gainesville, FL 32611 USA. EM jferrell@ufl.edu RI Webster, Theodore/A-4468-2009 OI Webster, Theodore/0000-0002-8259-2059 NR 31 TC 0 Z9 0 U1 2 U2 12 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD OCT-DEC PY 2015 VL 63 IS 4 BP 928 EP 935 DI 10.1614/WS-D-15-00062.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CT6XQ UT WOS:000362957200020 ER PT J AU Williams, MM AF Williams, Martin M., II TI Managing Weeds in Commercial Edamame Production: Current Options and Implications SO WEED SCIENCE LA English DT Article DE Competitive cultivars; hand weeding; immature soybean seed; minor crop; vegetable soybean ID SOYBEAN GLYCINE-MAX; SEED YIELD; L. MERR.; COMPETITION; INTERFERENCE; POPULATION; TOLERANCE; DIVERSITY; TRAITS AB Edamame, a specialty food-grade soybean popular among health-conscious consumers, is growing in popularity worldwide. Despite a well-developed soybean industry, most edamame consumed in the United States is imported from Asia. Considerable interest exists in growing edamame domestically; however, weed interference is a major problem, and until recently, only a single herbicide was registered for use on the crop. The objectives of this work were (1) to compare effectiveness of weed management treatments that utilize herbicides currently registered for use on edamame or that may be registered in the near future, (2) to determine the significance of edamame cultivar on performance of these treatments, and (3) to identify potential relationships between the crop and weed. Ten different weed management treatments were tested in three edamame cultivars over a 3-yr period. All weed management treatments increased marketable pod yield relative to the nontreated control, but only treatments with saflufenacil or S-metolachlor combinations were comparable to the hand-weeded weed-free treatment. Of the treatments studied, S-metolachlor followed by imazamox was among the greatest yielding, had the least weed density and biomass, and did not reduce crop population density. Also, cultivars differed in their weed-suppressive ability. Path analysis indicated certain relationships were consistent across cultivars, such as weed population density having a direct negative association with crop biomass; however, other edamame-weed interactions were not identical across cultivars. Although more improvements are needed, the vegetable industry is beginning to have nascent weed management options in edamame, which will likely reduce reliance on hand weeding and result in crop-production costs that are more competitive in the global market. Nomenclature: Imazamox; S-metolachlor; saflufenacil; edamame, Glycine max (L.) Merr. C1 Univ Illinois, USDA ARS, Global Change & Photosynth Res, Urbana, IL 61801 USA. RP Williams, MM (reprint author), Univ Illinois, USDA ARS, Global Change & Photosynth Res, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM martin.williams@ars.usda.gov NR 29 TC 0 Z9 0 U1 3 U2 11 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD OCT-DEC PY 2015 VL 63 IS 4 BP 954 EP 961 DI 10.1614/WS-D-15-00027.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CT6XQ UT WOS:000362957200023 ER PT J AU Muller, MJ Enderle, J Pourhassan, M Braun, W Eggeling, B Lagerpusch, M Gluer, CC Kehayias, JJ Kiosz, D Bosy-Westphal, A AF Mueller, Manfred James Enderle, Janna Pourhassan, Maryam Braun, Wiebke Eggeling, Benjamin Lagerpusch, Merit Glueer, Claus-Christian Kehayias, Joseph J. Kiosz, Dieter Bosy-Westphal, Anja TI Metabolic adaptation to caloric restriction and subsequent refeeding: the Minnesota Starvation Experiment revisited SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE body composition; energy balance; energy expenditure; weight change; metabolic adaptation; weight loss; starvation; refeeding; MRI ID RESTING ENERGY-EXPENDITURE; BODY-WEIGHT REGULATION; ADAPTIVE THERMOGENESIS; PHYSICAL-ACTIVITY; THYROID-HORMONES; HUMANS; LEPTIN; SEMISTARVATION; OBESITY; FAT AB Background: Adaptive thermogenesis (AT) is the fat-free mass (FFM)-independent reduction of resting energy expenditure (REE) to caloric restriction (CR). AT attenuates weight loss and favors weight regain. Its variance, dynamics, and control remain obscure. Objectives: Our aims were to address the variance and kinetics of AT, its associations with body composition in the context of endocrine determinants, and its effect on weight regain. Design: Thirty-two nonobese men underwent sequential overfeeding (1 wk at +50% of energy needs), CR (3 wk at -50% of energy needs), and refeeding (2 wk at +50% of energy needs). AT and its determinants were measured together with body composition as assessed with the use of quantitative magnetic resonance, whole-body MRI, isotope dilution, and nitrogen and fluid balances. Results: Changes in body weight were +1.8 kg (overfeeding), -6.0 kg (CR), and +3.5 kg (refeeding). CR reduced fat mass and FFM by 114 and 159 g/d, respectively. Within FFM, skeletal muscle (-5%), liver (-13%), and kidneys (-8%) decreased. CR also led to reductions in REE (-266 kcal/d), respiratory quotient (-15%), heart rate (-14%), blood pressure (-7%), creatinine clearance (-12%), energy cost of walking (-22%), activity of the sympathetic nervous system (SNS) (-38%), and plasma leptin (-44%), insulin (-54%), adiponectin (-49%), 3,5,3'-tri-iodo-thyronine (T3) (-39%), and testosterone (-11%). AT was 108 kcal/d or 48% of the decrease in REE. Changes in FFM composition explained 36 kcal, which left 72 kcal/d for true AT. The decrease in AT became significant at <= 3 d of CR and was related to decreases in insulin secretion (r = 0.92, P < 0.001), heart rate (r = 0.60, P < 0.05), creatinine clearance (r = 0.79, P < 0.05), negative fluid balance (r = 0.51, P < 0.01), and the free water clearance rate (r = -0.90, P < 0.002). SNS activity and plasma leptin, ghrelin, and T3 and their changes with CR were not related to AT. Conclusion: During early weight loss, AT is associated with a fall in insulin secretion and body fluid balance. C1 [Mueller, Manfred James; Enderle, Janna; Pourhassan, Maryam; Braun, Wiebke; Eggeling, Benjamin; Lagerpusch, Merit; Kiosz, Dieter] Univ Kiel, Inst Human Nutr & Food Sci, Kiel, Germany. [Glueer, Claus-Christian] Univ Kiel, Univ Med Ctr Schleswig Holstein, Mol Imaging North Competence Ctr, Clin Radiol & Neuroradiol,Biomed Imaging, Kiel, Germany. [Kehayias, Joseph J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Body Composit Lab, Boston, MA 02111 USA. [Bosy-Westphal, Anja] Univ Hohenheim, Inst Nutr Med, Stuttgart, Germany. RP Muller, MJ (reprint author), Univ Kiel, Inst Human Nutr & Food Sci, Kiel, Germany. EM mmueller@nutrfoodsc.uni-kiel.de FU German Ministry of Education and Research [BMBF 01EA1336]; German Research Foundation [DFG Bo 3296/1-1]; BMBF Kompetenznetz Adipositas, Core Domain "Body composition" (Korperzusammensetzung) [01GI1125] FX Supported by a grant of the German Ministry of Education and Research (BMBF 01EA1336), the German Research Foundation (DFG Bo 3296/1-1), and the BMBF Kompetenznetz Adipositas, Core Domain "Body composition" (Korperzusammensetzung; 01GI1125). NR 50 TC 16 Z9 17 U1 3 U2 22 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD OCT PY 2015 VL 102 IS 4 BP 807 EP 819 DI 10.3945/ajcn.115.109173 PG 13 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS7CO UT WOS:000362241900012 PM 26399868 ER PT J AU MacKay, DS Eck, PK Gebauer, SK Baer, DJ Jones, PJH AF MacKay, Dylan S. Eck, Peter K. Gebauer, Sarah K. Baer, David J. Jones, Peter J. H. TI CYP7A1-rs3808607 and APOE isoform associate with LDL cholesterol lowering after plant sterol consumption in a randomized clinical trial SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE cholesterol; gene-nutrient interactions; LDL cholesterol; nutrigenetics; plant sterols ID APOLIPOPROTEIN-E; HYPERCHOLESTEROLEMIC SUBJECTS; GENETIC-VARIATION; DEUTERIUM INCORPORATION; SERUM-LIPIDS; ABSORPTION; ESTERS; METABOLISM; HUMANS; STANOL AB Background: The benefits of plant sterols (PSs) for cholesterol lowering are hampered by large heterogeneity across individuals, potentially because of genetic polymorphisms. Objective: We investigated the impact of candidate genetic variations on cholesterol response to PSs in a trial that recruited individuals with high or low endogenous cholesterol synthesis, estimated by lathosterol to cholesterol (L:C) ratio. Design: Mildly hypercholesterolemic adults preselected as possessing either high endogenous cholesterol synthesis (n = 24; mean SEM: L:C ratio = 2.03 +/- 0.39 mu mol/mmol) or low endogenous cholesterol synthesis (n = 39; mean +/- SEM: L:C ratio = 0.99 +/- 0.28 mu mol/mmol) consumed 2 g PS/d or a placebo for 28 d by using a dual-center, single-blind, randomized crossover design. Cholesterol synthesis and change in cholesterol absorption were measured with stable isotopic tracers. Candidate single-nucleotide polymorphisms and apolipoprotein E (APOE) isoform were assessed by TaqMan genotyping assay. Results: The cholesterol fractional synthesis rate was higher (P < 0.001) in participants with high endogenous cholesterol synthesis (mean +/- SEM: placebo: 9.16% +/- 0.47%; PSs: 9.74% +/- 0.47%) than in participants with low endogenous cholesterol synthesis (mean +/- SEM placebo: 5.72% +/- 0.43%; PS: 7.10% +/- 0.43%). Low-density lipoprotein (LDL) cholesterol lowering in response to PSs was associated with individuals' genotypes. Cholesterol 7 alpha-hydroxylase (CYP7A1-rs3808607) T/T homozygotes showed no LDL cholesterol lowering (mean +/- SEM: -0.05 +/- 0.07 mmol/L, P = 0.9999, n = 20), whereas the presence of the G-allele associated with LDL cholesterol response in a dose-dependent fashion (mean +/- SEM G/T: -0.22 +/- 0.06 mmol/L, P = 0.0006, n = 35; G/G: -0.46 +/- 0.12 mmol/L, P = 0.0009, n = 8). Similarly, APOE 63 carriers (mean +/- SEM: -0.13 +/- 0.05 mmol/L, P = 0.0370, n = 40) responded less than APOE epsilon 4 carriers (mean +/- SEM: -0.31 +/- 0.07 mmol/L, P < 0.0001, n = 23). Moreover, genoset CYP7A1-rs3808607 T/T/APOE epsilon 3 was associated with nonresponsiveness (mean +/- SEM: +0.09 +/- 0.08 mmol/L, P = 0.9999, n = 14). rs5882 in cholesteryl ester transfer protein (CETP) and rs4148217 in ATP-binding cassette subfamily G member 8 (ABCG8) did not associate with LDL cholesterol lowering. Cholesterol absorption decreased as a result of PS consumption, but this decrease was not related to circulating LDL cholesterol concentrations, cholesterol synthesis phenotype, or genotypes. Conclusion: CYP7A1-rs3808607 and APOE isoform are associated with the extent of reduction in circulating LDL cholesterol in response to PS consumption and could serve as potential predictive genetic markers to identify individuals who would derive maximum LDL cholesterol lowering with PS consumption. C1 [MacKay, Dylan S.; Jones, Peter J. H.] ARS, Richardson Ctr Funct Foods & Nutraceut, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [MacKay, Dylan S.; Eck, Peter K.; Jones, Peter J. H.] ARS, Dept Human Nutr Sci, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Gebauer, Sarah K.; Baer, David J.] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Jones, Peter J. H.] Univ Manitoba, Dept Food Sci, Winnipeg, MB R3T 2N2, Canada. RP Jones, PJH (reprint author), ARS, Richardson Ctr Funct Foods & Nutraceut, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. EM peter.jones@umanitoba.ca OI Eck, Peter/0000-0003-2371-9774 FU Canadian Institutes of Health Research [(FRN) 83894] FX Supported by Canadian Institutes of Health Research funding reference number (FRN) 83894. Placebo and plant sterol margarines were provided by Unilever Canada Inc. NR 33 TC 3 Z9 3 U1 3 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD OCT PY 2015 VL 102 IS 4 BP 951 EP 957 DI 10.3945/ajcn.115.109231 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS7CO UT WOS:000362241900028 PM 26333513 ER PT J AU Webb, KM Calderon, FJ AF Webb, Kimberly M. Calderon, Francisco J. TI Mid-Infrared (MIR) and Near-Infrared (NIR) Detection of Rhizoctonia solani AG 2-2 IIIB on Barley-Based Artificial Inoculum SO APPLIED SPECTROSCOPY LA English DT Article DE Near-infrared spectroscopy; NIR spectroscopy; Mid-infrared spectroscopy; MIR spectroscopy; Rhizoctonia solani; R. solani; Pathogen detection ID FT-IR SPECTROSCOPY; SUGAR-BEET; ROOT-ROT; SOIL; IDENTIFICATION; POPULATION; RESISTANCE; INFECTION; DISEASE; LEAVES AB The amount of Rhizoctonia solani in the soil and how much must be present to cause disease in sugar beet (Beta vulgaris L.) is relatively unknown. This is mostly because of the usually low inoculum densities found naturally in soil and the low sensitivity of traditional serial dilution assays. We investigated the usefulness of Fourier transform mid-infrared (MIR) and near-infrared (NIR) spectroscopic properties in identifying the artificial colonization of barley grains with R. solani AG 2-2 IIIB and in detecting R. solani populations in plant tissues and inoculants. The objectives of this study were to compare the ability of traditional plating assays to NIR and MIR spectroscopies to identify R. solani in different-size fractions of colonized ground barley (used as an artificial inoculum) and to differentiate colonized from non-inoculated barley. We found that NIR and MIR spectroscopies were sensitive in resolving different barley particle sizes, with particles that were <0.25 and 0.25-0.5 mm having different spectral properties than coarser particles. Moreover, we found that barley colonized with R. solani had different MIR spectral properties than the non-inoculated samples for the larger fractions (0.5-1.0, 1.0-2.0, and >2.0 mm) of the ground barley. This colonization was confirmed using traditional plating assays. Comparisons with the spectra from pure fungal cultures and non-inoculated barley suggest that the MIR spectrum of colonized barley is different because of the consumption of C substrates by the fungus rather than because of the presence of fungal bands in the spectra of the colonized samples. We found that MIR was better than NIR spectroscopy in differentiating the colonized from the control samples. C1 [Webb, Kimberly M.] USDA ARS, Sugar Beet Res Unit, Crops Res Lab, Ft Collins, CO 80526 USA. [Calderon, Francisco J.] USDA ARS, Cent Great Plains Resources Management Res, Akron, CO 80720 USA. RP Calderon, FJ (reprint author), USDA ARS, Cent Great Plains Resources Management Res, 40335 Cty Rd GG, Akron, CO 80720 USA. EM Francisco.Calderon@ars.usda.gov FU Beet Sugar Development Foundation [454] FX Additional funding for this project was provided by the Beet Sugar Development Foundation project #454. We sincerely thank Travis Vagher for the protocol to colonize hulless barley using the mushroom-spawn bags for the artificial inoculum and suggestions to minimize contamination issues. In addition, we thank Addison Reed, Claire Freeman, and Katherine Schmidt for their assistance in preparing the testing materials and performing some of the experiments. We also thank Mark West for assistance in the statistical analysis of the traditional plating assays. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal-opportunity provider and employer. NR 42 TC 0 Z9 0 U1 3 U2 14 PU SOC APPLIED SPECTROSCOPY PI FREDERICK PA 5320 SPECTRUM DRIVE SUITE C, FREDERICK, MD 21703 USA SN 0003-7028 EI 1943-3530 J9 APPL SPECTROSC JI Appl. Spectrosc. PD OCT PY 2015 VL 69 IS 10 BP 1129 EP 1136 DI 10.1366/14-07727 PG 8 WC Instruments & Instrumentation; Spectroscopy SC Instruments & Instrumentation; Spectroscopy GA CT2FO UT WOS:000362617600002 PM 26449805 ER PT J AU Messer, KD Borchers, AM AF Messer, Kent D. Borchers, Allison M. TI Choice for goods under threat of destruction SO ECONOMICS LETTERS LA English DT Article DE Field experiment; Preference reversals; Cost effective conservation ID CONSERVATION; ECONOMICS; VALUES AB The choices related to preservation often involve consideration of the fate of the non-selected land. Yet, theory traditionally assumes that the fate of non-selected goods does not influence consumers' preferences. Results from a framed field experiment involving the private choice of wine show that consumer preferences can dramatically shift for items under the threat of imminent destruction. This shift (upwards of 58% increase) may explain why conservation professionals, despite decades of scientific evidence, have failed to adopt cost-effective techniques that would yield large conservation benefits at no additional cost. Interestingly, economists exhibit similar preference shifts. (c) 2015 Elsevier B.V. All rights reserved. C1 [Messer, Kent D.] Univ Delaware, Dept Appl Econ & Stat, Unidel H Cosgrove Chair Environm, Newark, DE 19716 USA. [Borchers, Allison M.] Econ Res Serv, USDA, Washington, DC 20250 USA. RP Messer, KD (reprint author), Univ Delaware, Dept Appl Econ & Stat, Unidel H Cosgrove Chair Environm, Newark, DE 19716 USA. EM messer@udel.edu FU National Science Foundation [EPS-0814251]; US Environmental Protection Agency [08001017]; US Department of Agriculture [59-6000-4-0064] FX The research was received financial support of the National Science Foundation (EPS-0814251), the US Environmental Protection Agency (08001017), and the US Department of Agriculture (59-6000-4-0064) for this research though no federal funds were spent on wine. NR 16 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 0165-1765 EI 1873-7374 J9 ECON LETT JI Econ. Lett. PD OCT PY 2015 VL 135 BP 137 EP 140 DI 10.1016/j.econlet.2015.07.026 PG 4 WC Economics SC Business & Economics GA CT2EW UT WOS:000362615800034 ER PT J AU Johnson, KD Birdsey, R Cole, J Swatantran, A O'Neil-Dunne, J Dubayah, R Lister, A AF Johnson, Kristofer D. Birdsey, Richard Cole, Jason Swatantran, Anu O'Neil-Dunne, Jarlath Dubayah, Ralph Lister, Andrew TI Integrating LIDAR and forest inventories to fill the trees outside forests data gap SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Trees outside forest; Carbon management; Nonforest biomass; LIDAR; Forest inventory ID CARBON STORAGE; UNITED-STATES; URBAN; AREAS; SEQUESTRATION AB Forest inventories are commonly used to estimate total tree biomass of forest land even though they are not traditionally designed to measure biomass of trees outside forests (TOF). The consequence may be an inaccurate representation of all of the aboveground biomass, which propagates error to the outputs of spatial and process models that rely on the inventory data. An ideal approach to fill this data gap would be to integrate TOF measurements within a traditional forest inventory for a parsimonious estimate of total tree biomass. In this study, Light Detection and Ranging (LIDAR) data were used to predict biomass of TOF in all "nonforest boolean AND Forest Inventory and Analysis (FIA) plots in the state of Maryland. To validate the LIDAR-based biomass predictions, a field crew was sent to measure TOF on nonforest plots in three Maryland counties, revealing close agreement at both the plot and county scales between the two estimates. Total tree biomass in Maryland increased by 25.5 Tg, or 15.6 %, when biomass of TOF were included. In two counties (Carroll and Howard), there was a 47 % increase. In contrast, counties located further away from the interstate highway corridor showed only a modest increase in biomass when TOF were added because nonforest conditions were less common in those areas. The advantage of this approach for estimating biomass of TOF is that it is compatible with, and explicitly separates TOF biomass from, forest biomass already measured by FIA crews. By predicting biomass of TOF at actual FIA plots, this approach is directly compatible with traditionally reported FIA forest biomass, providing a framework for other states to follow, and should improve carbon reporting and modeling activities in Maryland. C1 [Johnson, Kristofer D.; Birdsey, Richard; Cole, Jason; Lister, Andrew] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. [Swatantran, Anu; Dubayah, Ralph] Univ Maryland, Dept Geog Sci, College Pk, MD 20740 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Spatial Anal Lab, Burlington, VT 05405 USA. RP Johnson, KD (reprint author), US Forest Serv, USDA, No Res Stn, 11 Campus Blvd Ste 200, Newtown Sq, PA 19073 USA. EM kristoferdjohnson@fs.fed.us RI Swatantran, Anu/B-8786-2016 FU NASA [N4-CMS14-0037] FX David Bruhn and Kathleen Kranich were responsible for all the field work involved in the validation component of the study and supported additional office and data entry work. Matthew Patterson and Thomas Willard provided training to the field crew. Cassandra Olson, James Blehm, and Robert Ilgenfritz provided FIA plot information and valuable guidance. Two internal reviews by Lara Roman and Grant Domke provided valuable feedback to improve the manuscript. This project was supported by NASA grant N4-CMS14-0037 (Dubayah, principal investigator). NR 19 TC 0 Z9 0 U1 1 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD OCT PY 2015 VL 187 IS 10 AR 623 DI 10.1007/s10661-015-4839-1 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CS7SD UT WOS:000362284700018 PM 26364065 ER PT J AU Chen, HZ Cheng, Z Wisniewski, M Liu, YS Liu, J AF Chen, Huizhen Cheng, Zhe Wisniewski, Michael Liu, Yongsheng Liu, Jia TI Ecofriendly hot water treatment reduces postharvest decay and elicits defense response in kiwifruit SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Defense response; Fungal pathogens; Hot water treatment; Kiwifruit; Postharvest decay ID LOW-TEMPERATURE STORAGE; SWEET CHERRY FRUIT; BOTRYTIS-CINEREA; PENICILLIUM-EXPANSUM; SPORE GERMINATION; CHILLING INJURY; ACTINIDIA-DELICIOSA; DISEASE RESISTANCE; WASHING TREATMENT; OXIDATIVE STRESS AB Hot water treatment (HWT) of fruit is an effective approach for managing postharvest decay of fruits and vegetables. In the present study, the effects of HWT (45 A degrees C for 10 min) on the growth of Botrytis cinerea and Penicillium expansum in vitro, and gray (B. cinerea) and blue mold (P. expansum) development in kiwifruit were investigated. HWT effectively inhibited spore germination and germ tube elongation of B. cinerea and P. expansum. Reactive oxygen species accumulation and protein impairment in the fungi triggered by HWT contributed to the inhibitory effect. Results of in vivo studies showed that HWT controlled gray and blue mold in kiwifruit stored at 4 and 25 A degrees C. HWT induced a significant increase in the activity of antioxidant enzymes, including catalase and peroxidase, and the level of total phenolic compounds in kiwifruit. These findings indicate that the inhibition of postharvest decay in kiwifruit by HWT is associated with the inhibition of spore germination of both fungal pathogens and the elicitation of defense response in the kiwifruit host. Moreover, HWT used in this study did not impair fruit quality. HWT appears to represent a potential non-chemical alternative for the effective management of postharvest decay of kiwifruit. C1 [Chen, Huizhen; Cheng, Zhe; Liu, Yongsheng; Liu, Jia] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. [Wisniewski, Michael] USDA ARS, Kearneysville, WV 25430 USA. RP Liu, J (reprint author), Hefei Univ Technol, Sch Biotechnol & Food Engn, 193 Tunxi Rd, Hefei 230009, Peoples R China. EM jialiu1983@163.com FU Project from Department of Education, Anhui Province [KJ2014ZD25]; Project from National Natural Science Foundation of China [31471157]; Hefei University of Technology [58-1931-4-008FN]; USDA [58-1931-4-008FN] FX This work was supported by a Project from Department of Education, Anhui Province (No. KJ2014ZD25), a Project from National Natural Science Foundation of China (No. 31471157), and a Collaborative Project between Hefei University of Technology and USDA (No. 58-1931-4-008FN). NR 55 TC 1 Z9 1 U1 9 U2 32 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD OCT PY 2015 VL 22 IS 19 BP 15037 EP 15045 DI 10.1007/s11356-015-4714-1 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CS8IB UT WOS:000362329300062 PM 26002370 ER PT J AU Song, QJ Hyten, DL Jia, GF Quigley, CV Fickus, EW Nelson, RL Cregan, PB AF Song, Qijian Hyten, David L. Jia, Gaofeng Quigley, Charles V. Fickus, Edward W. Nelson, Randall L. Cregan, Perry B. TI Fingerprinting Soybean Germplasm and Its Utility in Genomic Research SO G3-GENES GENOMES GENETICS LA English DT Article DE soybean; germplasm; genotyping; SoySNP50K; genetic diversity; haplotype block map ID MAX L.-MERR.; QUANTITATIVE TRAIT LOCI; HAPLOTYPE BLOCKS; POD DEHISCENCE; SEED SIZE; POPULATION; DIVERSITY; SELECTION; QTL; ASSOCIATION AB The United States Department of Agriculture, Soybean Germplasm Collection includes 18,480 domesticated soybean and 1168 wild soybean accessions introduced from 84 countries or developed in the United States. This collection was genotyped with the SoySNP50K BeadChip containing greater than 50K single-nucleotide polymorphisms. Redundant accessions were identified in the collection, and distinct genetic backgrounds of soybean from different geographic origins were observed that could be a unique resource for soybean genetic improvement. We detected a dramatic reduction of genetic diversity based on linkage disequilibrium and haplotype structure analyses of the wild, landrace, and North American cultivar populations and identified candidate regions associated with domestication and selection imposed by North American breeding. We constructed the first soybean haplotype block maps in the wild, landrace, and North American cultivar populations and observed that most recombination events occurred in the regions between haplotype blocks. These haplotype maps are crucial for association mapping aimed at the identification of genes controlling traits of economic importance. A case-control association test delimited potential genomic regions along seven chromosomes that most likely contain genes controlling seed weight in domesticated soybean. The resulting dataset will facilitate germplasm utilization, identification of genes controlling important traits, and will accelerate the creation of soybean varieties with improved seed yield and quality. C1 [Song, Qijian; Hyten, David L.; Jia, Gaofeng; Quigley, Charles V.; Fickus, Edward W.; Cregan, Perry B.] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Hyten, David L.] Pioneer HiBred Int Inc, Johnston, IA 50131 USA. [Nelson, Randall L.] ARS, USDA, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. [Nelson, Randall L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Song, QJ (reprint author), ARS, Soybean Genom & Improvement Lab, Beltsville Agr Res Ctr W, USDA, Beltsville, MD 20705 USA. EM Qijian.Song@ars.usda.gov FU United Soybean Board [8265]; United Soybean Board FX We thank Dr. Donghe Xu at the Japan International Research Center for Agricultural Sciences, Tsukuba, Japan, and Dr. Mun Sup Yoon at the Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, Suwon, South Korea, for the identification of landrace genotypes from Japan and Korea. We thank Rob Parry and Chris Pooley for their technical support in assembling the necessary hardware and software required for the Illumina sequence analysis and David M. Grant at USDA-ARS, Ames, IA, for depositing the genotyping dataset in SoyBase. This research was supported with funding from the United Soybean Board Project #8265. The support of the United Soybean Board is greatly appreciated. NR 35 TC 18 Z9 19 U1 2 U2 15 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD OCT 1 PY 2015 VL 5 IS 10 BP 1999 EP 2006 DI 10.1534/g3.115.019000 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA CT0SB UT WOS:000362505600007 PM 26224783 ER PT J AU Bian, Y Holland, JB AF Bian, Yang Holland, James B. TI Ensemble Learning of QTL Models Improves Prediction of Complex Traits SO G3-GENES GENOMES GENETICS LA English DT Article DE quantitative trait loci; thinning and aggregating; ensemble modeling; Zea mays ID ASSOCIATION MAPPING POPULATION; MARKER-ASSISTED SELECTION; SOUTHERN LEAF-BLIGHT; GENOMIC SELECTION; GENETIC ARCHITECTURE; QUANTITATIVE TRAITS; MAIZE; EFFICIENCY; RESISTANCE; REGRESSION AB Quantitative trait locus (QTL) models can provide useful insights into trait genetic architecture because of their straightforward interpretability but are less useful for genetic prediction because of the difficulty in including the effects of numerous small effect loci without overfitting. Tight linkage between markers introduces near collinearity among marker genotypes, complicating the detection of QTL and estimation of QTL effects in linkage mapping, and this problem is exacerbated by very high density linkage maps. Here we developed a thinning and aggregating (TAGGING) method as a new ensemble learning approach to QTL mapping. TAGGING reduces collinearity problems by thinning dense linkage maps, maintains aspects of marker selection that characterize standard QTL mapping, and by ensembling, incorporates information from many more markers-trait associations than traditional QTL mapping. The objective of TAGGING was to improve prediction power compared with QTL mapping while also providing more specific insights into genetic architecture than genome-wide prediction models. TAGGING was compared with standard QTL mapping using cross validation of empirical data from the maize (Zea mays L.) nested association mapping population. TAGGING-assisted QTL mapping substantially improved prediction ability for both biparental and multifamily populations by reducing both the variance and bias in prediction. Furthermore, an ensemble model combining predictions from TAGGING-assisted QTL and infinitesimal models improved prediction abilities over the component models, indicating some complementarity between model assumptions and suggesting that some trait genetic architectures involve a mixture of a few major QTL and polygenic effects. C1 [Bian, Yang; Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Holland, James B.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Holland, JB (reprint author), USDA ARS, Raleigh, NC 27695 USA. EM james_holland@ncsu.edu OI Holland, James/0000-0002-4341-9675 FU United States National Science Foundation [IOS-1238014, IOS-0820619]; US Department of Agriculture, Agricultural Research Service; National Science Foundation [IOS-1238014]; Monsanto Co. fellowship FX We thank Dr. Jason Peiffer for suggesting use of the Nelder-Mead optimization technique and for providing us with HapMap v1-based relationship matrix for the NAM panel. We thank Dr. Howard Bondell for a helpful discussion about analyses on bias and variance. We used the High Performance Computing Services in NC State University and thank Dr. Gary Howell for assistance with parallel computing. We appreciate two anonymous reviewers for their thorough evaluations. Research was supported by United States National Science Foundation grants IOS-1238014 and IOS-0820619 and the US Department of Agriculture, Agricultural Research Service. Y.B. is a graduate student at NC State University and supported by a National Science Foundation grant IOS-1238014 and a Monsanto Co. fellowship. NR 46 TC 0 Z9 0 U1 2 U2 10 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD OCT 1 PY 2015 VL 5 IS 10 BP 2073 EP 2084 DI 10.1534/g3.115.021121 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA CT0SB UT WOS:000362505600015 PM 26276383 ER PT J AU Bari, E Nazarnezhad, N Kazemi, SM Ghanbary, MAT Mohebby, B Schmidt, O Clausen, CA AF Bari, Ehsan Nazarnezhad, Nouredin Kazemi, Seyed Mahmoud Ghanbary, Mohammad Ali Tajick Mohebby, Behbood Schmidt, Olaf Clausen, Carol A. TI Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Pleurotus ostreatus; Trametes versicolor; Fagus orientalis; Mass loss; Compression strength; Hardness; Impact load resistance; FT-IR spectroscopy; Chemical analysis; Microscopy ID BROWN-ROT; INFRARED-SPECTROSCOPY; CHEMICAL-COMPOSITION; DECAY; LIGNIN; LIGNOCELLULOSICS; CHROMATOGRAPHY; STRENGTH AB The degradation capabilities of two white rot fungi, Pleurotus ostreatus and Trametes versicolor, obtained from natural stands of Fagus orientalis (beech) in Northern Iran were studied. Fruiting bodies of P. ostreatus and T versicolor were collected from fallen beech in the Alamdardeh forest, Iran. Beech wood samples were exposed to both fungi for 120 days according to EN113. Mass loss, resistance to compression parallel to grain, impact load resistance, as well as lignin, cellulose and hemicellulose content were determined in the samples before and after fungal exposure. FT-IR spectroscopy was used to collect spectra after fungal decay. Light and electron microscopy were used to study white-rot decay patterns in wood and compare the mechanism of attack for both fungi. Both fungi reduced wood mass and chemical constituents in similar quantities. Chemical analyses indicated similar reductions in cellulose, lignin and total carbohydrates. Both fungi had negligible affect on the hemicelluloses, arabinan, galactan, and glucan. Patterns of white decay were the same for both fungi. Thinning of the cell walls, colonization of fungal hyphae as well as formation of bore holes on the cell wallswere also similar for both fungi. The results demonstrated that both fungi produced a simultaneous white rot in beech wood. (c) 2015 Elsevier Ltd. All rights reserved. C1 [Bari, Ehsan; Nazarnezhad, Nouredin; Kazemi, Seyed Mahmoud] Univ Agr Sci & Nat Resources, Fac Nat Resources, Dept Wood & Paper Sci, Sari, Iran. [Bari, Ehsan] Tech Fac Sari 2, Dept Wood Sci & Engn, Sari, Iran. [Ghanbary, Mohammad Ali Tajick] Agr & Nat Resources Univ, Coll Agron Sci, Dept Mycol & Plant Pathol, Sari, Iran. [Mohebby, Behbood] Tarbiat Modares Univ, Fac Nat Resources, Dept Wood & Paper Sci, Noor, Iran. [Schmidt, Olaf] Univ Hamburg, Dept Wood Biol, D-21031 Hamburg, Germany. [Clausen, Carol A.] USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Bari, E (reprint author), Univ Agr Sci & Nat Resources, Fac Nat Resources, Dept Wood & Paper Sci, Sari, Iran. EM bari_Jenzites@yahoo.com; m.tajick@sanru.ac.ir; mohebbyb@modare.ac.ir; olaf.schmidt@uni-hamburg.de; cclausen@fs.fed.us OI Bari, Ehsan/0000-0002-9144-0663 NR 53 TC 9 Z9 9 U1 6 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD OCT PY 2015 VL 104 BP 231 EP 237 DI 10.1016/j.ibiod.2015.03.033 PG 7 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA CS8AP UT WOS:000362308400033 ER PT J AU Bari, E Oladi, R Schmidt, O Clausen, CA Ohno, K Nicholas, DD Daryaei, MG Karim, M AF Bari, Ehsan Oladi, Reza Schmidt, Olaf Clausen, Carol A. Ohno, Katie Nicholas, Darrel D. Daryaei, Mehrdad Ghodskhah Karim, Maryam TI Influence of xylem ray integrity and degree of polymerization on bending strength of beech wood decayed by Pleurotus ostreatus and Trametes versicolor SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Xylem ray; Degree of polymerization; White rot fungi; Impact bending strength ID SOUTHERN PINE; FUNGI; DEGRADATION; MODEL AB The scope of this research was to evaluate the influence of xylem ray (XR) and degree of polymerization (DP) of holocellulose in Oriental beech wood (Fagus orientalis Lipsky.) on impact bending strength against two white-rot fungi. Beech wood specimens, exposed to Pleurotus ostreatus and Trametes versicolor, were evaluated for impact bending strength at 15-day intervals for 120 days. Microscopic evaluations at each time interval indicated that both fungi degraded marginal ray parenchyma at early stages of decomposition causing a drastic loss in impact bending strength. However, the two fungi differed in their ability to reduce DP; DP and xylem ray integrity (without precedence) were the most important factors in mechanical stability of beech wood. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Bari, Ehsan] Sari Agr Sci & Nat Resources Univ, Dept Wood & Paper Sci, Sari, Iran. [Bari, Ehsan] Tech Fac Sari 2, Dept Wood Sci & Engn, Sari, Iran. [Oladi, Reza] Univ Tehran, Fac Nat Resources, Dept Wood Sci & Technol, Karaj, Iran. [Schmidt, Olaf] Univ Hamburg, Dept Wood Biol, D-21031 Hamburg, Germany. [Clausen, Carol A.; Ohno, Katie] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Nicholas, Darrel D.] Mississippi State Univ, Dept Sustainable Bioprod, Mississippi State, MS 39762 USA. [Daryaei, Mehrdad Ghodskhah; Karim, Maryam] Univ Guilan, Fac Nat Resources, Dept Forestry, Guilan, Iran. RP Bari, E (reprint author), Sari Agr Sci & Nat Resources Univ, Dept Wood & Paper Sci, Sari, Iran. EM bari_lenzites@yahoo.com; oladi@ut.ac.ir; o.schmidt@uni-hamburg.de; cclausen@fs.fed.us; kohno@fs.fed.us; d.nicholas@msstate.edu; mdaryaei9@gmail.com; maryam.karim88@gmail.com OI Bari, Ehsan/0000-0002-9144-0663; Oladi, Reza/0000-0002-8522-7321 NR 49 TC 4 Z9 4 U1 1 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD OCT PY 2015 VL 104 BP 299 EP 306 DI 10.1016/j.ibiod.2015.04.019 PG 8 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA CS8AP UT WOS:000362308400042 ER PT J AU Lebow, S Arango, R Woodward, B Lebow, P Ohno, K AF Lebow, Stan Arango, Rachel Woodward, Bessie Lebow, Patricia Ohno, Katie TI Efficacy of alternatives to zinc naphthenate for dip treatment of wood packaging materials SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Wood preservatives; Zinc naphthentate; Copper; Azole; Dip treatment; Decay fungi; Termites ID PRESERVATIVES AB Research is being conducted to evaluate potential preservatives to replace zinc naphthenate (ZnN) for use in dip-treatment of wooden packaging materials. In this study, laboratory tests evaluated the efficacy of preservatives in protecting Southern pine and yellow poplar against decay fungi and termites. Nine preservatives were evaluated at one or two concentrations, and in some cases with two dip times. The results of this study indicate that higher concentrations of most of the preservatives evaluated will provide protection similar to or greater than that of ZnN. Four of the formulations provided protection equivalent to or greater than that of ZnN across all of the test organisms. Lower concentrations of some of the copper-based preservatives were less effective than ZnN in preventing colonization of Southern pine by the copper tolerant fungus Postia placenta, but most formulations were more effective than ZnN in protecting yellow poplar against white-rot fungi. The test formulations tended to be more effective than ZnN in preventing termite feeding on southern pine, but some were less effective than ZnN in protecting yellow poplar from termites. Efficacy was found to increase with preservative concentration in several cases, but did not increase with a longer dip time. Published by Elsevier Ltd. C1 [Lebow, Stan; Arango, Rachel; Woodward, Bessie; Lebow, Patricia; Ohno, Katie] US Forest Serv, USDA, Forest Prod Lab, Washington, DC 20250 USA. RP Lebow, S (reprint author), US Forest Serv, USDA, Forest Prod Lab, Washington, DC 20250 USA. EM slebow@fs.fed.us FU US Army Armament Research Development and Engineering Center FX The authors acknowledge funding and technical support from the US Army Armament Research Development and Engineering Center. NR 16 TC 2 Z9 2 U1 1 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD OCT PY 2015 VL 104 BP 371 EP 376 DI 10.1016/j.ibiod.2015.07.006 PG 6 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA CS8AP UT WOS:000362308400050 ER PT J AU Xiao, ZL Bauchan, G Nichols-Russell, L Luo, YG Wang, Q Nou, XW AF Xiao, Zhenlei Bauchan, Gary Nichols-Russell, Lydia Luo, Yaguang Wang, Qin Nou, Xiangwu TI Proliferation of Escherichia coli O157:H7 in Soil-Substitute and Hydroponic Microgreen Production Systems SO JOURNAL OF FOOD PROTECTION LA English DT Article ID FRESH-PRODUCE; SPROUTS; LETTUCE; INTERNALIZATION; SAFETY; SEEDS; WASH; L. AB Radish (Raphanus sativus var. longipinnatus) microgreens were produced from seeds inoculated with Escherichia coli O157:H7 by using peat moss based soil-substitute and hydroponic production systems. E. coli populations on the edible and inedible parts of harvested microgreen plants (7 days postseeding) and in growth medium were examined. E. coli O157:H7 was shown to survive and proliferate significantly during microgreen growth in both production systems, with a higher level in the hydroponic production system. At the initial seed inoculation level of 3.7 log CFU/g, E. coli O157:H7 populations on the edible part of microgreen plants reached 2.3 and 2.1 log CFU/g (overhead irrigation and bottom irrigation, respectively) for microgreens from the soil-substitute production system and reached 5.7 log CFU/g for those hydroponically grown. At a higher initial inoculation of 5.6 log CFU/g seeds, the corresponding E. coli O157:117 populations on the edible parts of microgreens grown in these production systems were 3.4, 3.6, and 5.3 log CFU/g, respectively. Examination of the spatial distribution of bacterial cells on different parts of microgreen plants showed that contaminated seeds led to systematic contamination of whole plants, including both edible and inedible parts, and seed coats remained the focal point of E. coli O157:H7 survival and growth throughout the period of microgreen production. C1 [Xiao, Zhenlei; Nichols-Russell, Lydia; Luo, Yaguang; Nou, Xiangwu] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Bauchan, Gary] ARS, USDA, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA. [Xiao, Zhenlei; Wang, Qin] Univ Maryland, Dept Food Sci & Nutr, College Pk, MD 20740 USA. RP Nou, XW (reprint author), ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM xiangwu.nou@ars.usda.gov FU USDA-National Institute of Food and Agriculture Specialty Crop Research Initiative Grant [2010-51181-21230]; Friends of Agricultural Research-Beltsville (Maryland) FX This research is partially supported by USDA-National Institute of Food and Agriculture Specialty Crop Research Initiative Grant (award 2010-51181-21230). L. Nichols-Russell is an undergraduate student intern from University Maryland supported by Friends of Agricultural Research-Beltsville (Maryland). NR 21 TC 1 Z9 1 U1 4 U2 13 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD OCT PY 2015 VL 78 IS 10 BP 1785 EP 1790 DI 10.4315/0362-028X.JFP-15-063 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CT2DB UT WOS:000362611100002 PM 26408126 ER PT J AU Oni, RA Sharma, M Buchanan, RL AF Oni, Ruth A. Sharma, Manan Buchanan, Robert L. TI Survival of Salmonella enterica in Dried Turkey Manure and Persistence on Spinach Leaves SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157H7; WATER ACTIVITY; SEROVAR ENTERITIDIS; FRESH PRODUCE; BACTERIA; SOIL; INTERNALIZATION; TYPHIMURIUM; POPULATIONS; OPERATIONS AB Concerns about the microbiological safety of fresh produce have attracted attention in the past three decades due to multiple foodborne outbreaks. Animal manure contaminated with enteric pathogens has been identified as an important preharvest pathogen source. This study investigated the survival of Salmonella enterica in dust particles of dehydrated turkey manure and how association with manure dust may enhance the survival of salmonellae on leafy greens in the field. The survival of a cocktail of multiple Salmonella serotypes in the dried fecal material of various particle sizes (125 to 500 mu m) was examined at varying moisture contents (5, 10, and 15%). Survival times of the pathogen were inversely related to moisture content and particle size of manure dust, with viable Salmonella still detectable for up to 291 days in the smallest particle size (125 mu m) with 5% moisture. Association with manure dust particles increased the survival of Salmonella when subjected to UV light both under laboratory conditions and on the surface of spinach leaves in a greenhouse setting. The results of this study suggest that aerosolized manure particles could be a potential vehicle for Salmonella dispersal to leafy greens if the microorganism is present in the dry manure. C1 [Oni, Ruth A.; Buchanan, Robert L.] Univ Maryland, Coll Agr & Nat Resources, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Buchanan, Robert L.] Univ Maryland, Coll Agr & Nat Resources, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Sharma, Manan] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Area Res Ctr, Beltsville, MD 20705 USA. RP Buchanan, RL (reprint author), Univ Maryland, Coll Agr & Nat Resources, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM rbuchana@umd.edu FU Specialty Crop Research Initiative from USDA's National Institute of Food and Agriculture (USDA-NlFA) [2011-51181-30767] FX The authors acknowledge Miao Guo at the Nutrition and Food Science Department of the University of Maryland, and Dr. Yanguang Luo and Ellen Turner at the USDA Environmental Microbial and Food Safety Laboratory, Beltsville, MD, for helping with the statistical analysis of this work. We also thank Cheryl Roberts, Isaac Onigbinde, Sean Ferguson, and Janet Akinduro for their assistance with sample collection and laboratory analysis. This work was supported by the Specialty Crop Research Initiative (grant 2011-51181-30767) from the USDA's National Institute of Food and Agriculture (USDA-NlFA). The sponsor played no role in the study design and implementation. Any opinions, findings, and conclusions expressed in this material are those of the authors and do not necessarily reflect the views of the USDA-NlFA. NR 36 TC 2 Z9 2 U1 0 U2 8 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD OCT PY 2015 VL 78 IS 10 BP 1791 EP 1799 DI 10.4315/0362-028X.JFP-15-047 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CT2DB UT WOS:000362611100003 PM 26408127 ER PT J AU Oscar, TP AF Oscar, T. P. TI Neural Network Model for Survival and Growth of Salmonella enterica Serotype 8,20:-:z(6) in Ground Chicken Thigh Meat during Cold Storage: Extrapolation to Other Serotypes SO JOURNAL OF FOOD PROTECTION LA English DT Article ID DOMESTIC REFRIGERATORS; RISK-ASSESSMENT; RAW CHICKEN; TYPHIMURIUM; TEMPERATURE; VALIDATION; RETAIL; POULTRY; BREAST; TIME AB Mathematical models that predict the behavior of human bacterial pathogens in food are valuable tools for assessing and managing this risk to public health. A study was undertaken to develop a model for predicting the behavior of Salmonella enterica serotype 8,20:-:z(6) in chicken meat during cold storage and to determine how well the model would predict the behavior of other serotypes of Salmonella stored under the same conditions. To develop the model, ground chicken thigh meat (0.75 cm(3)) was inoculated with 1.7 log Salmonella 8,20:-:z(6) and then stored for 0 to 8 days at -8 to 16 degrees C. An automated miniaturized most-probable-number (MPN) method was developed and used for the enumeration of Salmonella. Commercial software (Excel and the add-in program NeuralTools) was used to develop a multilayer feedforward neural network model with one hidden layer of two nodes. The performance of the model was evaluated using the acceptable prediction zone (APZ) method. The number of Salmonella in ground chicken thigh meat stayed the same (P > 0.05) during 8 days of storage at -8 to 8 degrees C but increased (P < 0.05) during storage at 9 degrees C (+0.6 log) to 16 degrees C (+5.1 log). The proportion of residual values (observed minus predicted values) in an APZ (pAPZ) from -1 log (fail-safe) to 0.5 log (fail-dangerous) was 0.939 for the data (n = 426 log MPN values) used in the development of the model. The model had a pAPZ of 0.944 or 0.954 when it was extrapolated to test data (n = 108 log MPN per serotype) for other serotypes (S. enterica serotype Typhimurium var 5-, Kentucky, Typhimurium, and Thompson) of Salmonella in ground chicken thigh meat stored for 0 to 8 days at -4, 4, 12, or 16 degrees C under the same experimental conditions. A pAPZ of >= 0.7 indicates that a model provides predictions with acceptable bias and accuracy. Thus, the results indicated that the model provided valid predictions of the survival and growth of Salmonella 8,20:-:z(6) in ground chicken thigh meat stored for 0 to 8 days at -8 to 16 degrees C and that the model was validated for extrapolation to four other serotypes of Salmonella. C1 Univ Maryland Eastern Shore, USDA, Residue Chem & Predict Microbiol Res Unit, Agr Res Serv,Ctr Food Sci & Technol, Princess Anne, MD 21853 USA. RP Oscar, TP (reprint author), Univ Maryland Eastern Shore, USDA, Residue Chem & Predict Microbiol Res Unit, Agr Res Serv,Ctr Food Sci & Technol, Room 2111, Princess Anne, MD 21853 USA. EM thomas.oscar@ars.usda.gov NR 32 TC 2 Z9 2 U1 0 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD OCT PY 2015 VL 78 IS 10 BP 1819 EP 1827 DI 10.4315/0362-028X.JFP-15-093 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CT2DB UT WOS:000362611100006 PM 26408130 ER PT J AU DellaValle, DM Glahn, RP Shaff, JE O'Brien, K AF DellaValle, Diane M. Glahn, Raymond P. Shaff, Jon E. O'Brien, Kimberly TI Iron Absorption from an Intrinsically Labeled Lentil Meal Is Low but Upregulated in Women with Poor Iron Status SO JOURNAL OF NUTRITION LA English DT Article DE pulses; iron; bioavailability; lentils; absorption; non-heme ID YOUNG-WOMEN; LENS-CULINARIS; BIOAVAILABILITY; FOOD; BIOFORTIFICATION; POPULATION; BANGLADESH; CHILDREN; HEPCIDIN; ISOTOPE AB Background: Low iron absorption from important staple foods may contribute to iron deficiency in developing countries. To date, few studies have examined the iron bioavailability of pulse crops as commonly prepared and consumed by humans. Objective: The objectives were to characterize the iron absorption from a test meal of intrinsically labeled Fe-57 lentils prepared as dal, to compare the bioavailability of iron from Fe-57 in dal with that observed for a reference dose of Fe-58 as ferrous sulfate, and to assess associations between iron absorption and iron status indicators. Methods: This crossover study included 19 nonpregnant women (n= 6 anemic; hemoglobin: <12.0 g/dL) who consumed 2 test meals on consecutive days in a counter-balanced order, ferrous sulfate (7 mg FeSO4 plus 1 mg Fe-58) and 330 g dal (lentils enriched to 85.1% with Fe-57, 8 mg native Fe-57). Iron absorption was determined by analyzing blood samples taken 14 d after dosing with the use of magnetic sector thermal ionization mass spectrometry. Results: We found that the mean iron absorption from the dal was 2.20% +/- 3.40% and was significantly lower than the 23.6% +/- 13.2% observed from the same iron load given as ferrous sulfate (P < 0.001). Absorption of non-heme iron from dal and from ferrous sulfate was inversely associated with serum ferritin (SF; r= 0.50, P= 0.05 and r= 0.81, P< 0.001, respectively) and serum hepcidin (r= 0.45, P= 0.05 and r= 0.60, P= 0.007, respectively). Anemic women absorbed more iron from either source (1.20% from dal, P= 0.10; 18.3% from ferrous sulfate, P= 0.001) compared with women who were iron replete. Conclusions: Iron absorption from the dal was low overall but upregulated in anemic women. Both SF and hepcidin were inversely associated with iron absorption from both a supplemental and a food-based non-heme iron source in nonanemic and anemic women. C1 [DellaValle, Diane M.; Glahn, Raymond P.; Shaff, Jon E.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [O'Brien, Kimberly] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. RP DellaValle, DM (reprint author), Marywood Univ, Dept Nutr & Dietet, Neill Ctr Healthy Families 221, Scranton, PA 18509 USA. EM ddellavalle@hotmail.com OI DellaValle, Diane/0000-0002-3855-432X FU Saskatchewan Pulse Growers; USDA Agricultural Research Service FX Supported by the Saskatchewan Pulse Growers and USDA Agricultural Research Service NR 25 TC 1 Z9 1 U1 3 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD OCT PY 2015 VL 145 IS 10 BP 2253 EP 2257 DI 10.3945/jn.115.217273 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS9CW UT WOS:000362387700009 PM 26338889 ER PT J AU Raatz, SK Johnson, LK Picklo, MJ AF Raatz, Susan K. Johnson, LuAnn K. Picklo, Matthew J. TI Consumption of Honey, Sucrose, and High-Fructose Corn Syrup Produces Similar Metabolic Effects in Glucose-Tolerant and -Intolerant Individuals SO JOURNAL OF NUTRITION LA English DT Article DE sucrose; honey; high-fructose corn syrup; glycemia; triglycerides ID SUGAR-SWEETENED BEVERAGES; DIETARY FRUCTOSE; CARDIOVASCULAR-DISEASE; INSULIN-RESISTANCE; BLOOD-PRESSURE; OBESITY; HEALTH; PLASMA; RISK; HYPERTRIGLYCERIDEMIA AB Background: Public health recommendations call for a reduction in added sugars; however, controversy exists over whether all nutritive sweeteners produce similar metabolic effects. Objective: The objective was to compare the effects of the chronic consumption of 3 nutritive sweeteners [honey, sucrose, and high-fructose corn syrup containing 55% fructose (HFCS55)] on circulating glucose, insulin, lipids, and inflammatory markers; body weight; and blood pressure in individuals with normal glucose tolerance (GT) and those with impaired glucose tolerance (IGT). Methods: In a crossover design, participants consumed daily, in random order, 50 g carbohydrate from assigned sweeteners for 2 wk with a 2-to 4-wk washout period between treatments. Participants included 28 GT and 27 IGT volunteers with a mean age of 38.9 +/- 3.6 y and 52.1 +/- 2.7 y, respectively, and a body mass index (in kg/m(2)) of 26 +/- 0.8 and 31.5 +/- 1.0, respectively. Body weight, blood pressure (BP), serum inflammatory markers, lipids, fasting glucose and insulin, and oral-glucose-tolerance tests (OGTTs) were completed pre- and post-treatment. The OGTT incremental areas under the curve (iAUCs) for glucose and insulin were determined and homeostasis model assessment of insulin resistance (HOMA-IR) scores were calculated. Results: Body weight and serum glucose, insulin, inflammatory markers, and total and LDL-cholesterol concentrations were significantly higher in the IGT group than in the GT group at baseline. Glucose, insulin, HOMA-IR, and the OGTT iAUC for glucose or insulin did not differ by treatment, but all responses were significantly higher in the IGT group compared with the GT group.. Body weight was unchanged by treatment. Systolic BP was unchanged, whereas diastolic BP was significantly lower in response to sugar intake across all treatments. An increase in high-sensitivity C-reactive protein (hsCRP) was observed in the IGT group in response to all sugars. No treatment effect was observed for interleukin 6. HDL cholesterol did not differ as a result of status or treatment. Triglyceride (TG) concentrations increased significantly from pre- to post-treatment in response to all sugars tested. Conclusions: Daily intake of 50 g carbohydrate from honey, sucrose, or H FCS55 for 14 d resulted in similar effects on measures of glycemia, lipid metabolism, and inflammation. All 3 increased TG concentrations in both GT and IGT individuals and elevated glycemic and inflammatory responses in the latter. This trial was registered at clinicaltrials.gov as NCT01371266. C1 [Raatz, Susan K.; Johnson, LuAnn K.; Picklo, Matthew J.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Raatz, Susan K.] Univ Minnesota, Dept Nutr & Food Sci, St Paul, MN 55108 USA. RP Raatz, SK (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM susan.raatz@ars.usda.gov FU National Honey Board; USDA Agricultural Research Service [5450-51000-048-00D] FX Supported by a grant from the National Honey Board and by the USDA Agricultural Research Service (5450-51000-048-00D). NR 36 TC 1 Z9 1 U1 14 U2 71 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD OCT PY 2015 VL 145 IS 10 BP 2265 EP 2272 DI 10.3945/jn.115.218016 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS9CW UT WOS:000362387700011 PM 26338891 ER PT J AU Hileman, C Dirajlal-Fargo, S Lam, SK Kumar, J Lacher, C Combs, GF McComsey, GA AF Hileman, Corrilynn Dirajlal-Fargo, Sahera Lam, Suet Kam Kumar, Jessica Lacher, Craig Combs, Gerald F., Jr. McComsey, Grace A. TI Plasma Selenium Concentrations Are Sufficient and Associated with Protease Inhibitor Use in Treated HIV-Infected Adults SO JOURNAL OF NUTRITION LA English DT Article DE selenium; human immunodeficiency virus; inflammation; immune activation; cardiovascular disease ID ACUTE MYOCARDIAL-INFARCTION; RANDOMIZED CONTROLLED-TRIAL; INTIMA-MEDIA THICKNESS; ANTIRETROVIRAL THERAPY; MONOCYTE ACTIVATION; TRACE-ELEMENTS; BETA-CAROTENE; T-CELL; DISEASE; SERUM AB Background: Selenium is an essential constituent of selenoproteins, which play a substantial role in antioxidant defense and inflammatory cascades. Selenium deficiency is associated with disease states characterized by inflammation, including cardiovascular disease (CVD). Although HIV infection has been associated with low selenium, the role of selenium status in HIV-related CVD is unclear. Objectives: We sought to assess associations between plasma selenium and markers of inflammation, immune activation, and subclinical vascular disease in HIV-infected adults on contemporary antiretroviral therapy (ART) and to determine if statin therapy modifies selenium status. Methods: In the Stopping Atherosclerosis and Treating Unhealthy bone with RosuvastatiN trial, HIV-infected adults on stable ART were randomly assigned 1:1 to rosuvastatin or plkebo. Plasma selenium concentrations were determined at entry, week 24, and week 48. Spearman correlation and linear regression analyses were used to assess relations between baseline selenium, HIV-related factors and markers of inflammation, immune activation, and subclinical vascular disease. Changes in selenium over 24 and 48 wk were compared between groups. Results: One hundred forty-seven HIV-infected adults were included. All participants were on ART. Median current CD4+ count was 613, and 76% had HIV-1 RNA <= 5.48 copies/mL (range: <20-600). Median plasma selenium concentration was 122 mu g/L (range: 62-200). At baseline, higher selenium was associated with protease inhibitor (PI) use, lower body mass index, and a higher proportion of activated CD8+ T cells (CD8+CD38+human leukocyte antigen-DR+), but not markers of inflammation or subclinical vascular disease. Over 48 wk, selenium concentrations increased in the statin group (P < 0.01 within group), but the change did not differ between groups (+13.1 vs. +5.3 mu g/L; P= 0.14 between groups). Conclusions: Plasma selenium concentrations were within the normal range for the background population and were not associated with subclinical vascular disease in HIV-infected adults on contemporary ART. The association between current PI use and higher selenium may have implications for ART allocation, especially in resource-limited countries. Also, it appears that statin therapy may increase selenium concentrations; however, larger studies are necessary to confirm this finding. This trial was registered at clinicaltrials.gov as NCT01218802. C1 [Hileman, Corrilynn] Case Western Reserve Univ, Metrohlth Med Ctr, Dept Med, Div Infect Dis, Cleveland, OH USA. [Hileman, Corrilynn; Dirajlal-Fargo, Sahera; McComsey, Grace A.] Case Western Reserve Univ, Sch Med, Cleveland, OH 44106 USA. [Dirajlal-Fargo, Sahera; Lam, Suet Kam; Kumar, Jessica; McComsey, Grace A.] Case Western Reserve Univ, Rainbow Babies & Childrens Hosp, Dept Pediat, Div Pediat Infect Dis & Rheumatol, Cleveland, OH 44106 USA. [Lacher, Craig; Combs, Gerald F., Jr.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP McComsey, GA (reprint author), Case Western Reserve Univ, Sch Med, Cleveland, OH 44106 USA. EM grace.mccomsey@case.edu FU NIH [NR012642, K23HL116209]; USDA Agricultural Research Service FX This study was supported by the NIH [grant no. NR012642 (to GAM) and K23HL116209 (to COH)] and the USDA Agricultural Research Service. Technical assistance was provided by the Center for AIDS Research, Case Western Reserve University (P30 AI36219). NR 45 TC 1 Z9 1 U1 1 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD OCT PY 2015 VL 145 IS 10 BP 2293 EP 2299 DI 10.3945/jn.115.214577 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS9CW UT WOS:000362387700015 PM 26269240 ER PT J AU Van Rompay, MI McKeown, NM Goodman, E Eliasziw, M Chomitz, VR Gordon, CM Economos, CD Sacheck, JM AF Van Rompay, Maria I. McKeown, Nicola M. Goodman, Elizabeth Eliasziw, Misha Chomitz, Virginia R. Gordon, Catherine M. Economos, Christina D. Sacheck, Jennifer M. TI Sugar-Sweetened Beverage Intake Is Positively Associated with Baseline Triglyceride Concentrations, and Changes in Intake Are Inversely Associated with Changes in HDL Cholesterol over 12 Months in a Multi-Ethnic Sample of Children SO JOURNAL OF NUTRITION LA English DT Article DE dyslipidemias; sweetening agents; ethnic groups; health status disparities; child ID FOOD FREQUENCY QUESTIONNAIRE; CARDIOMETABOLIC RISK-FACTORS; BLOCK KIDS QUESTIONNAIRE; UNITED-STATES; CONSUMPTION PATTERNS; INSULIN-RESISTANCE; DRINK CONSUMPTION; PHYSICAL-ACTIVITY; YOUNG-CHILDREN; BLOOD-PRESSURE AB Background: Intake of sugar-sweetened beverages (SSBs) is linked to greater cardiometabolic risk in adults. Although longitudinal evidence is sparse among children, SSB intake reduction is targeted to reduce cardiometabolic risk factors in this group. Objective: We investigated characteristics associated with consumption of SSBs in a multi-ethnic sample of children/ adolescents and measured cross-sectional and longitudinal associations between SSB intake and plasma HDL cholesterol and triglycerides (TGs) over 12 mo. Methods: In a diverse cohort of children aged 8-15 y, cross-sectional associations (n = 613) between baseline SSB intake and blood lipid concentrations and longitudinal associations (n = 380) between mean SSB intake, changes in SSB intake, and lipid changes over 12 mo were assessed with multivariable linear regression. Results: Greater SSB intake was associated with lower socioeconomic status, higher total energy intake, lower fruit/vegetable intake, and more sedentary time. In cross-sectional analysis, greater SSB intake was associated with higher plasma TG concentrations among consumers (62.4, 65.3, and 71.6 mg/dL in children who consumed >0 but <2, >= 2 but <7, and >= 7 servings/wk, respectively; P-trend: 0.03); plasma HDL cholesterol showed no cross-sectional association. In the longitudinal analysis, mean SSB intake over 12 mo was not associated with lipid changes; however, the 12-mo increase in plasma HDLcholesterol concentration was greater among children who decreased their intake by >= 1 serving/wk (4.6 +/- 0.8 mg/dL) compared with children whose intake stayed the same (2.0 +/- 0.8 mg/dL) or increased (1.5 +/- 0.8 mg/dL; P = 0.02). Conclusions: In a multi-ethnic sample of children, intake of SSBs was positively associated with TG concentrations among consumers, and changes in SSB intake were inversely associated with HDL cholesterol concentration changes over 12 mo. Further research in large diverse samples of children is needed to study the public health implications of reducing SSB intake among children of different raciaVethnic groups. The Daily D Health Study was registered at clinicaltnals.gov as NCT01537809. C1 [Van Rompay, Maria I.; Economos, Christina D.; Sacheck, Jennifer M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Sch Med, Boston, MA 02111 USA. [Eliasziw, Misha; Chomitz, Virginia R.] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. [Goodman, Elizabeth] MassGen Hosp Children, Boston, MA USA. [Goodman, Elizabeth] Harvard Univ, Sch Med, Boston, MA USA. [Gordon, Catherine M.] Brown Univ, Hasbro Childrens Hosp, Alpert Med Sch, Div Adolescent Med, Providence, RI 02912 USA. [Gordon, Catherine M.] Brown Univ, Hasbro Childrens Hosp, Alpert Med Sch, Div Endocrinol, Providence, RI 02912 USA. RP Van Rompay, MI (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. EM maria.vanrompay@tufts.edu FU NIH National Heart, Lung, and Blood Institute [R01HL106160] FX Supported by NIH National Heart, Lung, and Blood Institute grant R01HL106160. NR 52 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD OCT PY 2015 VL 145 IS 10 BP 2389 EP 2395 DI 10.3945/jn.115.212662 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS9CW UT WOS:000362387700027 PM 26338888 ER PT J AU Zeng, HW Claycombe, KJ Reindl, KM AF Zeng, Huawei Claycombe, Kate J. Reindl, Katie M. TI Butyrate and deoxycholic acid play common and distinct roles in HCT116 human colon cell proliferation SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Apoptosis; Butyrate; Colon cancer; Cell cycle; Deoxycholic acid ID CHAIN FATTY-ACIDS; INDUCED APOPTOSIS; CANCER RISK; DNA-DAMAGE; BILE-ACIDS; ACTIVATION; ROS; CARCINOGENESIS; FERMENTATION; EXPRESSION AB Consumption of a high-fat diet causes an increase in bile acid deoxycholic acid (DCA) in colon lumen and colon cancer risk, while butyrate, an intestinal microbiota metabolite of dietary fiber, has been shown to exhibit colon cancer-preventive effects. To distinguish these opposing effects of DCA and butyrate (two major metabolites in colon lumen), we examined the effects of physiologically relevant doses of butyrate (0.5-2 mmol/l) and DCA (0.05-0.3 mmol/l) on colon cell proliferation. We hypothesize that butyrate and DCA each modulates the cell cycle and apoptosis via common and distinct cellular signaling targets. In this study, we demonstrated that both butyrate and DCA inhibited cell proliferation by up to 89% and 92% and increased cell apoptosis rate by up to 3.1- and 4.5-fold, respectively. Cell cycle analyses revealed that butyrate led to an increase in G1 and G2 fractions with a concomitant drop in the S-phase fraction, but DCA induced an increase in only G1 fraction with a concomitant drop in the S-phase fraction when compared with the untreated cells. The examination of early cellular signaling revealed that DCA but not butyrate increased intracellular reactive oxygen species, genomic DNA breakage, the activation of ERK1/2, caspase-3 and PARP. In contrast DCA decreased activated Rb protein level, and butyrate but not DCA increased p21 expression. Collectively, although both butyrate and DCA inhibit colonic cell proliferation, butyrate increases tumor suppressor gene expression, whereas DCA decreases tumor suppressor activation in cell cycle and apoptosis pathways. Published by Elsevier Inc. C1 [Zeng, Huawei; Claycombe, Kate J.] USDA, ARS, GFHNRC, Grand Forks, ND 58203 USA. [Reindl, Katie M.] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58108 USA. RP Zeng, HW (reprint author), USDA, ARS, GFHNRC, 2420 2nd Ave,North, Grand Forks, ND 58203 USA. EM huawei.zeng@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service, CRIS project [5450-51000-045-00] FX We greatly appreciate Mary Briske-Anderson, Brenda Skinner, Kay Keehr and Karen LoneFight for the technical support. This work was funded by the US Department of Agriculture, Agricultural Research Service, CRIS project (5450-51000-045-00). NR 38 TC 0 Z9 0 U1 6 U2 17 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD OCT PY 2015 VL 26 IS 10 BP 1022 EP 1028 DI 10.1016/j.jnutbio.2015.04.007 PG 7 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA CS9CZ UT WOS:000362388000005 PM 26026836 ER PT J AU Singh, SK Reddy, VR AF Singh, Shardendu K. Reddy, Vangimalla R. TI Response of carbon assimilation and chlorophyll fluorescence to soybean leaf phosphorus across CO2: Alternative electron sink, nutrient efficiency and critical concentration SO JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY LA English DT Article DE Chlorophyll; Carotenoids; Photosynthesis; Quantum yield; Photorespiration ID C-3 PHOTOSYNTHESIS; MESOPHYLL LIMITATIONS; CARBOXYLASE-OXYGENASE; SPECIFICITY FACTOR; MINERAL-NUTRITION; PHOTOSYSTEM-II; GAS-EXCHANGE; IN-VIVO; DIOXIDE; LEAVES AB To evaluate the response of CO2 assimilation rate (P-N) and various chlorophyll fluorescence (CF) parameters to phosphorus (P) nutrition, soybean plants were grown in controlled environment with sufficient (0.50 mM) and deficient (0.10 and 0.01 mM) phosphate (P) supply under ambient and elevated CO2 (aCO(2), 400 and eCO(2), 800 mu mol mol(-1), respectively). Measurements were made at ambient (21%) and low (2%) O-2 concentrations. Results showed strong correlation of leaf P concentration with P-N and CF parameters. The P deficiency showed parallel decreases in P-N, and CF parameters including quantum efficiency (F-v'/F-m'), quantum yield of photosystem II (Phi(PSII)), electron transport rate (J(F)), and photochemical quenching (q(p)). The F-v'/F-m' decreased as a result of greater decline in maximal (F-m') than minimal (F-o') fluorescence. The eCO(2) stimulated P-N especially under higher leaf P concentrations. Low O-2 also stimulated P-N but only at aCO(2). The photosynthetic carbon reduction (PCR, signified by P-N) and photorespiratory carbon oxidation cycles (PCO, signified photorespiration as indicated by ratio of J(F) to gross P-N and % increase in P-N at 2% O-2) was the major electron sinks. However, the presence of alternative electron sink was also evident as determined by the difference between the electron transport calculated from chlorophyll fluorescence and gas exchange measurements. Alternative electron sink declined at lower leaf P concentration suggesting its minor role in photochemical energy consumption, thus dissipation of the excess excitation pressure of PSII reaction center under P deficiency. The J(F)/P-G and % increase in P-N at 2 versus 21% O-2 remained consistent across leaf P concentration suggesting PCO cycle as an important mechanism to dissipate excess excitation energy in P deficient leaves. The severe decline of F-v'/F-m' Phi(PSII), J(F)and q(p) under P deficiency also suggested the occurrences of excess radiant energy dissipation by non-photochemical quenching mechanisms. Critical leaf P concentration (CLPC) needed to achieve 90% of the maximum value was greater for P-N than CF parameters. Moreover, CLPC was always higher at eCO(2) suggesting increased sensitivity of soybean to P deficiency under eCO(2). An increased phosphorus utilization efficiency of P-N and CF parameters was also achieved but with the expense of net CO2 assimilation in P-deficient leaves. (C) 2015 Elsevier B.V. All rights reserved. C1 [Singh, Shardendu K.; Reddy, Vangimalla R.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Singh, Shardendu K.] Univ Maryland, Wye Res & Educ Ctr, Queenstown, MD USA. RP Singh, SK (reprint author), USDA ARS, Crop Syst & Global Change Lab, Bldg 001,Rm 342,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM shardendu.singh@ars.usda.gov NR 55 TC 1 Z9 1 U1 3 U2 27 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 1011-1344 J9 J PHOTOCH PHOTOBIO B JI J. Photochem. Photobiol. B-Biol. PD OCT PY 2015 VL 151 BP 276 EP 284 DI 10.1016/j.jphotobiol.2015.08.021 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA CT2BT UT WOS:000362607700037 PM 26343044 ER PT J AU Sung, J Sonn, Y Lee, Y Kang, S Ha, S Krishnan, HB Oh, TK AF Sung, Jwakyung Sonn, Yeonkyu Lee, Yejin Kang, Seongsoo Ha, Sangkeun Krishnan, Hari B. Oh, Taek-Keun TI Compositional changes of selected amino acids, organic acids, and soluble sugars in the xylem sap of N, P, or K-deficient tomato plants SO JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE LA English DT Article DE nutrient deficiency; amino acids; organic acids; soluble sugars; tomato ID ARABIDOPSIS-THALIANA; BEAN-PLANTS; METABOLISM; STRESS; RESPONSES; METABOLOMICS; DEPRIVATION; PATHWAYS; DROUGHT; CULTURE AB Xylem sap plays a major role in long-distance transport of water, nutrients, and metabolites. However, there is little information on the behavior of metabolites in mineral-deficient xylem sap. For this reason, the time-dependent changes in selected metabolites (amino acids, organic acids, and soluble sugars) from tomato xylem sap in response to nitrogen (N), phosphorus (P), or potassium (K)-deficient condition were investigated. Tomato plants (Solanum lycopersicum L.) were grown hydroponically in liquid culture under three different mineral regimes: N-deficient [0.5 mM Ca(NO3)(2) and 0.5 mM KNO3], P-deficient (0.05 mM KH2PO4), and K-deficient (0.5 mM KNO3), respectively. Xylem sap was collected at 10:00 am after 1, 5, 15, and 30 d, and the selected metabolites were analyzed with liquid chromatography. All N, P, or K deficiencies led to a substantial increase in metabolites in the xylem sap. The predominant amino acid in the xylem sap was glutamine and, interestingly, all mineral deficiencies resulted in a substantial amount of -aminobutyric acid (GABA). Additionally, organic acids (citrate and malate) and soluble sugars were strongly increased in all mineral deficiencies, and, in particular, the level of shikimate was greatly affected by N deficiency. Based on these data, it is necessary to clearly elucidate an unknown event taking place in xylem loading in a variety of environmental impacts, and we are now studying to expand our knowledge on metabolic and proteomic responses using GC-MS and LC-MS. C1 [Sung, Jwakyung; Sonn, Yeonkyu; Lee, Yejin; Kang, Seongsoo; Ha, Sangkeun] Natl Acad Agr Sci, RDA, Div Soil & Fertilizer, Wonju 565851, North Jeolla Pr, South Korea. [Krishnan, Hari B.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Krishnan, Hari B.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. [Oh, Taek-Keun] Chungnam Natl Univ, Dept Bioenvironm Chem, Taejon 305764, South Korea. RP Oh, TK (reprint author), Chungnam Natl Univ, Dept Bioenvironm Chem, Taejon 305764, South Korea. EM ok5382@cnu.ac.kr FU Chungnam National University FX This study was financially supported by research fund of Chungnam National University. NR 31 TC 1 Z9 1 U1 15 U2 50 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1436-8730 EI 1522-2624 J9 J PLANT NUTR SOIL SC JI J. Plant Nutr. Soil Sci. PD OCT PY 2015 VL 178 IS 5 BP 792 EP 797 DI 10.1002/jpln.201500071 PG 6 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA CT1QQ UT WOS:000362575900012 ER PT J AU Brechenmacher, L Nguyen, THN Zhang, N Jun, TH Xu, D Mian, MAR Stacey, G AF Brechenmacher, Laurent Nguyen, Tran Hong Nha Zhang, Ning Jun, Tae-Hwan Xu, Dong Mian, M. A. Rouf Stacey, Gary TI Identification of Soybean Proteins and Genes Differentially Regulated in Near Isogenic Lines Differing in Resistance to Aphid Infestation SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE Soybean; aphid; transcriptomic; proteomic; NIL; resistant; susceptible ID BREVICORYNE-BRASSICAE ATTACK; WRKY TRANSCRIPTION FACTORS; RNA-SEQ REVEALS; DEFENSE RESPONSES; STATISTICAL-MODEL; MASS-SPECTROMETRY; FEEDING-BEHAVIOR; PLANT DEFENSE; PEA APHID; EXPRESSION AB Soybean aphid is an important pest causing significant yield losses. The Rag2 locus confers resistance to soybean aphid biotypes 1 and 2. Transcriptomic and proteomic analyses were done over a 48 h period after aphid infestation using near isogenic lines (NILs) differing at the Rag2 locus. Comparing the Rag2 and/or rag2 lines identified 3445 proteins, of which 396 were differentially regulated between the two lines, including proteins involved in cell wall metabolism, carbohydrate metabolism, and stress response. RNA-seq transcriptomic analysis identified 2361 genes significantly regulated between the resistant and susceptible lines. Genes upregulated in the Rag2 line were annotated as being involved in cell wall, secondary, and hormone metabolism as well as in stress, signaling, and transcriptional responses. Genes downregulated in the Rag2 line were annotated as being involved in photosynthesis and carbon metabolism. Interestingly, two genes (unknown and mitochondrial protease) located within the defined Rag2 locus were expressed significantly higher in the resistant genotype. The expression of a putative NBS-LRR resistant gene within the Rag2 locus was not different between the two soybean lines, but a second NBL-LRR gene located just at the border of the defined Rag2 locus was. Therefore, this gene may be a candidate R gene controlling aphid resistance. C1 [Brechenmacher, Laurent; Nguyen, Tran Hong Nha; Stacey, Gary] Univ Missouri, Natl Ctr Soybean Biotechnol, Div Biochem, Columbia, MO 65211 USA. [Brechenmacher, Laurent; Nguyen, Tran Hong Nha; Stacey, Gary] Univ Missouri, Natl Ctr Soybean Biotechnol, Div Plant Sci, Columbia, MO 65211 USA. [Zhang, Ning; Xu, Dong] Univ Missouri, Christopher S Bond Life Sci Ctr, Dept Comp Sci, Columbia, MO 65211 USA. [Jun, Tae-Hwan; Mian, M. A. Rouf] Ohio State Univ, USDA ARS, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Stacey, G (reprint author), Univ Missouri, Natl Ctr Soybean Biotechnol, Div Biochem, Columbia, MO 65211 USA. EM staceyg@missouri.edu OI Zhang, Ning/0000-0002-1218-5044 FU United Soybean Board; National Science Foundation [DBI-0421620] FX Work was funded by a grant from the United Soybean Board (to M.A.R.M. and G.S.). Salary support for L.B. and T.H.N.N. was provided by a National Science Foundation, Plant Genome grant Grant DBI-0421620 (to G.S.). NR 53 TC 0 Z9 0 U1 4 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 EI 1535-3907 J9 J PROTEOME RES JI J. Proteome Res. PD OCT PY 2015 VL 14 IS 10 BP 4137 EP 4146 DI 10.1021/acs.jproteome.5b00146 PG 10 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CS9BW UT WOS:000362385100005 PM 26350764 ER PT J AU Moorhead, J Gowda, P Hobbins, M Senay, G Paul, G Marek, T Porter, D AF Moorhead, Jerry Gowda, Prasanna Hobbins, Mike Senay, Gabriel Paul, George Marek, Thomas Porter, Dana TI Accuracy Assessment of NOAA Gridded Daily Reference Evapotranspiration for the Texas High Plains SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE climate; water resources management; Ogallala Aquifer region ID ENERGY BALANCE APPROACH; MAPPING EVAPOTRANSPIRATION; MODELS; SYSTEM AB The National Oceanic and Atmospheric Administration (NOAA) provides daily reference evapotranspiration (ETref) maps for the contiguous United States using climatic data from North American Land Data Assimilation System (NLDAS). This data provides large-scale spatial representation of ETref, which is essential for regional scale water resources management. Data used in the development of NOAA daily ETref maps are derived from observations over surfaces that are different from short (grass ETos) or tall (alfalfa ETrs) reference crops, often in nonagricultural settings, which carries an unknown discrepancy between assumed and actual conditions. In this study, NOAA daily ETos and ETrs maps were evaluated for accuracy, using observed data from the Texas High Plains Evapotranspiration (TXHPET) network. Daily ETos, ETrs and the climatic data (air temperature, wind speed, and solar radiation) used for calculating ETref were extracted from the NOAA maps for TXHPET locations and compared against ground measurements on reference grass surfaces. NOAA ETref maps generally overestimated the TXHPET observations (1.4 and 2.2mm/day ETos and ETrs, respectively), which may be attributed to errors in the NLDAS modeled air temperature and wind speed, to which reference ETref is most sensitive. Therefore, a bias correction to NLDAS modeled air temperature and wind speed data, or adjustment to the resulting NOAA ETref, may be needed to improve the accuracy of NOAA ETref maps. C1 [Moorhead, Jerry; Gowda, Prasanna] ARS CPRL, USDA, Bushland, TX 79012 USA. [Senay, Gabriel] US Geol Survey, Earth Resources Observat & Sci Ctr, Sioux Falls, SD 57030 USA. [Paul, George; Marek, Thomas] Texas A&M AgriLife Res, North Res Field, Etter, Amarillo, TX 79106 USA. [Porter, Dana] Texas A&M AgriLife Res & Extens Serv, Lubbock, TX 79403 USA. RP Moorhead, J (reprint author), ARS CPRL, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM jed.moorhead@ars.usda.gov OI Paul, George/0000-0002-5322-7650 NR 27 TC 1 Z9 1 U1 2 U2 4 PU WILEY PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD OCT PY 2015 VL 51 IS 5 BP 1262 EP 1271 DI 10.1111/1752-1688.12303 PG 10 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CS8WG UT WOS:000362369900008 ER PT J AU McEntire, M Snyder, S Freeman, D AF McEntire, Matthew Snyder, Scott Freeman, Don TI Comparison of Growth Between Morone Hybrids (Palmetto and Sunshine) in Earthen Ponds SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID BY-PRODUCT MEAL; STRIPED BASS; COMMERCIAL DIETS; SAXATILIS MALE; BODY-COMPOSITION; WHITE BASS; CHRYSOPS; PROTEIN; STRESS AB Palmetto bass are produced by crossing the female striped bass, Morone saxatilis, with the male white bass, Morone chrysops; whereas, the sunshine bass is the reciprocal cross. The hybrid striped bass industry typically rears sunshine bass in earthen ponds, because of the ease of handling, availability, and early maturation of the white bass female broodstock. Growth performance has been assumed similar between the crosses. Under commercial pond conditions, sunshine bass (19.8 +/- 0.4g (mean +/- SEM)) and palmetto bass (23.2 +/- 0.3g) were grown to market size (617.0 +/- 17.2g sunshine and 620 +/- 3.6g palmetto) within 15mo, yielding 4532.6kg/ha. The net production between the crosses was not significantly different and averaged 4373.4kg/ha. Feed conversion ratio (FCR) of sunshine bass (FCR 1.70) was better (P=0.0158) than palmetto bass (FCR 1.76). Commercial processing metrics of each cross demonstrated significant differences, with sunshine bass exhibiting lower frame waste (P=0.0457) than palmetto bass, probably due to fish body shape or conformational differences at this size. Without growth performance differences between the hybrids, there might be an advantage to rearing sunshine bass due to the slightly better FCR and increased skinless boneless fillet yield. C1 [McEntire, Matthew; Snyder, Scott; Freeman, Don] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP McEntire, M (reprint author), USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM Matthew.McEntire@ARS.USDA.GOV FU USDA/ARS [6225-31630-006-00D] FX The gift of sunshine bass by Keo Fish Farm, Keo, AR, USA, and palmetto bass by Andrew H. Hulsey AGFC State Hatchery, Hot Springs, AR, USA, is gratefully acknowledged. The authors thank Steven Rawles for valuable comments on the manuscript. This study was funded by the USDA/ARS under project number 6225-31630-006-00D. Animal care and experimental protocols were approved by the Harry K. Dupree Stuttgart National Aquaculture Research Center Institutional Animal Care and Use Committee and conformed to Agricultural Research Service Policies and Procedures 130.4 and 635.1. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 31 TC 0 Z9 0 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD OCT PY 2015 VL 46 IS 5 BP 557 EP 563 DI 10.1111/jwas.12213 PG 7 WC Fisheries SC Fisheries GA CS9TR UT WOS:000362435500009 ER PT J AU Gilbert, AT McCracken, GF Sheeler, LL Muller, LI O'Rourke, D Kelch, WJ New, JC AF Gilbert, Amy T. McCracken, Gary F. Sheeler, Lorinda L. Muller, Lisa I. O'Rourke, Dorcas Kelch, William J. New, John C., Jr. TI RABIES SURVEILLANCE AMONG BATS IN TENNESSEE, USA, 1996-2010 SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Bat; exposure; rabies; surveillance ID UNITED-STATES; NATIONAL SURVEILLANCE; EPIDEMIOLOGY; VIRUS; ECOLOGY; HOST; ESTABLISHMENT; COLORADO; DISEASE; HUMANS AB Rabies virus (RABV) infects multiple bat species in the Americas, and enzootic foci perpetuate in bats principally via intraspecific transmission. In recent years, bats have been implicated in over 90% of human rabies cases in the US. In Tennessee, two human cases of rabies have occurred since 1960: one case in 1994 associated with a tricolored bat (Perimyotis subflavus) RABV variant and another in 2002 associated with the tricolored/silver-haired bat (P. subflavus/Lasionycteris noctivagans) RABV variant. From 1996 to 2010, 2,039 bats were submitted for rabies testing in Tennessee. Among 1,943 bats in satisfactory condition for testing and with a reported diagnostic result, 96% (1,870 of 1,943) were identified to species and 10% (196 of 1,943) were rabid. Big brown (Eptesicus fuscus), tricolored, and eastern red (Lasiurus borealis) bats comprised 77% of testable bat submissions and 84% of rabid bats. For species with five or more submissions during 1996-2010, the highest proportion of rabid bats occurred in hoary (Lasiurus cinereus; 46%), unspecified Myotis spp. (22%), and eastern red (17%) bats. The best model to predict rabid bats included month of submission, exposure history of submission, species, and sex of bat. C1 [Gilbert, Amy T.; McCracken, Gary F.] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA. [Sheeler, Lorinda L.] South Coll, Knoxville, TN 37909 USA. [Muller, Lisa I.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [O'Rourke, Dorcas] E Carolina Univ, Dept Comparat Med, Greenville, NC 27834 USA. [Kelch, William J.; New, John C., Jr.] Univ Tennessee, Coll Vet Med, Knoxville, TN 37996 USA. RP Gilbert, AT (reprint author), USDA, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Amy.T.Gilbert@aphis.usda.gov OI Muller, Lisa/0000-0001-7833-2273; McCracken, Gary/0000-0002-2493-8103 FU US Environmental Protection Agency FX We thank the regional testing laboratories in the state of Tennessee, and particularly John Dunn, Rand Carpenter, Alice Green, and Emily Mosites, for technical advice and assistance during the study. This research was largely made possible by students from the College of Veterinary Medicine and the Department of Forestry, Wildlife and Fisheries, who participated in the annual Bat Identification Lab at the University of Tennessee. We also thank Justin Fischer for assistance with map illustrations. A.T.G. was supported by a US Environmental Protection Agency Science-To-Achieve-Results Graduate Fellowship. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of their respective institutions. NR 44 TC 1 Z9 1 U1 2 U2 22 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD OCT PY 2015 VL 51 IS 4 BP 821 EP 832 DI 10.7589/2014-12-277 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA CS9VP UT WOS:000362441100003 PM 26251992 ER PT J AU Berentsen, AR Johnson, SR Gilbert, AT VerCauteren, KC AF Berentsen, Are R. Johnson, Shylo R. Gilbert, Amy T. VerCauteren, Kurt C. TI Exposure to Rabies in Small Indian Mongooses (Herpestes auropunctatus) from Two Regions in Puerto Rico SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Caribbean; Herpestes auropunctatus; mongoose; Puerto Rico; rabies; wildlife disease ID UNITED-STATES; SURVEILLANCE; GRENADA; FOREST AB The small Indian mongoose (Herpestes auropunctatus) was introduced to several Caribbean Islands to control rat (Rattus spp.) damage to sugarcane plantations. Mongooses failed at suppressing rat populations and are now considered pests throughout most of their introduced range. Importantly, mongooses are rabies reservoirs on several Caribbean Islands. In Puerto Rico, mongooses have been implicated in up to 70% of reported animal rabies cases. There is no rabies vaccination program for wildlife in Puerto Rico, and data on rabies in mongooses are limited. We conducted a serosurvey of mongooses in two different ecologic environments in Puerto Rico: El Yunque National Forest and Cabo Rojo National Wildlife Refuge. We collected 119 serum samples from 112 mongooses, 44 (39.3%) of which were positive for rabies virus-neutralizing antibodies. We also collected oral swabs from 147 mongooses, including 88 from which we also collected serum. No oral swabs were positive for rabies virus RNA. Our data support previous research suggesting rabies virus is circulating within the mongoose population on Puerto Rico. C1 [Berentsen, Are R.; Johnson, Shylo R.; Gilbert, Amy T.; VerCauteren, Kurt C.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Berentsen, AR (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Are.R.Berentsen@aphis.usda.gov NR 19 TC 1 Z9 1 U1 3 U2 11 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD OCT PY 2015 VL 51 IS 4 BP 896 EP 900 DI 10.7589/2015-01-016 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA CS9VP UT WOS:000362441100014 PM 26251987 ER PT J AU Riley, DG Burke, JM Chase, CC Coleman, SW AF Riley, D. G. Burke, J. M. Chase, C. C., Jr. Coleman, S. W. TI Genetic effects for reproductive performance of straightbred and crossbred Romosinuano and Angus cows in a temperate zone SO LIVESTOCK SCIENCE LA English DT Article DE Criollo breeds; Crossbreeding; Heterosis; Reproductive rate ID X ENVIRONMENT INTERACTIONS; INFECTED TALL FESCUE; BEEF-CATTLE; COMMON BERMUDAGRASS; PREWEANING TRAITS; HEREFORD STEERS; BRAHMAN; CALVES; MILK; ERGOTAMINE AB The objectives of this work were to: (1) estimate heterosis and breed direct effects for cow reproduction traits of Romosinuano, Angus, and F-1 cows in a temperate climate, and (2) assess the effects of the type of forage grazed (bermudagrass, endophyte-infected and endophyte-free tall fescue) during the breeding season on traits and genetic effects. Calving and weaning rate (n=666 records) were evaluated for 145 cows from 2005 through 2012. Calving interval (n=400) and day of conception within breeding season (n=547) were recorded. Mixed models included combinations of fixed effects: year of record or cow age within year of record, breed type parameterized as main and interaction effects of sire breed and dam breed of cows with records. Additive genetic effects were included as a random component The sire breed-dam breed interaction was characterized by lower (P < 0.005) Angus calving and weaning rates (0.75 +/- 0.03, 0.65 +/- 0.04, respectively) than other groups, which ranged from 0.88 +/- 0.02 (Romosinuano) to 0.92 +/- 0.04 (Romosinuano-sired F-1) for calving rate and ranged from 0.81 +/- 0.03 (F-1 sired by Angus) to 0.82 +/- 0.03 (Romosinuano-sired F-1) for weaning rate. Romosinuano cows conceived later (P < 0.02) in the breeding season (25.2 +/- 2.3 d) than other groups (range from 16.9 +/- 3.1 to 17.3 +/- 1.8 d). Calving and weaning rates were highest (P < 0.05) for cows grazing bermudagrass during the breeding season (0.98 +/- 0.02, 0.93 +/- 0.03), and lowest for cows grazing endophyte-infected tall fescue (0.85 +/- 0.03, 0.78 +/- 0.04); cows grazing endophyte-free tall fescue were intermediate (0.94 +/- 0.02, 0.88 +/- 0.03). No interaction of forage type with sire or dam breed was detected (P > 0.24). Estimates of heterosis were 0.09 +/- 0.03, 0.11 +/- 0.04, and -3.7 +/- 2.0 d (P < 0.05) for calving and weaning rate, and conception day within breeding season, respectively. Romosinuano direct effects for these traits were large (P < 0.003) and favorable for calving and weaning rate (0.14 +/- 0.05, 0.2 +/- 0.05), but un-favorable for conception day within breeding season (8.7 +/- 2.9 d; P < 0.05). Angus cows had the greatest proportion of calf loss (0.33) and cows that died or were culled (0.8). Romosinuano may merit consideration for inclusion in breeding programs across forage types in temperate climates. (C) 2015 Published by Elsevier B.V. C1 [Riley, D. G.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Burke, J. M.] ARS, Dale Bumpers Small Farms Res Ctr, USDA, Booneville, AR 72927 USA. [Chase, C. C., Jr.] ARS, US Meat Anim Res Ctr, USDA, Clay Ctr, NE 68933 USA. [Coleman, S. W.] ARS, Grazinglands Res Lab, USDA, El Reno, OK 73036 USA. RP Riley, DG (reprint author), Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. EM david-riley@tamu.edu NR 29 TC 1 Z9 1 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD OCT PY 2015 VL 180 BP 22 EP 26 DI 10.1016/j.livsci.2015.06.024 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA CS9AT UT WOS:000362382200004 ER PT J AU Yesiltas, M Peale, RE Unger, M Sedlmair, J Hirschmugl, CJ AF Yesiltas, Mehmet Peale, Robert E. Unger, Miriam Sedlmair, Julia Hirschmugl, Carol J. TI Organic and inorganic correlations for Northwest Africa 852 by synchrotron-based Fourier transform infrared microspectroscopy SO METEORITICS & PLANETARY SCIENCE LA English DT Article ID DIFFUSE INTERSTELLAR-MEDIUM; TAGISH LAKE METEORITE; INTERPLANETARY DUST PARTICLES; CARBONACEOUS CHONDRITES; AQUEOUS ALTERATION; SILICATE GRAINS; CR CHONDRITES; SOLAR-SYSTEM; MURCHISON METEORITE; WATER-ICE AB Relationships between organic molecules and inorganic minerals are investigated in a single 34 mu m diameter grain of the CR2 chondrite Northwest Africa 852 (NWA) 852 with submicron spatial resolution using synchrotron-based imaging micro-FTIR spectroscopy. Correlations based on absorption strength for the various constituents are determined using statistical correlation analysis. The silicate band is found to be correlated with the hydration band, and the latter is highly correlated with stretching modes of aliphatic hydrocarbons. Spatial distribution maps show that water+ organic combination, silicate, OH, and C-H distributions overlap, suggesting a possible catalytic role of phyllosilicates in the formation of organics. In contrast, the carbonate band is anticorrelated with water+organic combination, however uncorrelated with any other spectral feature. The average ratio of asymmetric CH2 and CH3 band strengths (CH2/CH3 = 2.53) for NWA 852 is similar to the average ratio of interplanetary dust particles (similar to 2.40) and Wild 2 cometary dust particles (2.50), but it significantly exceeds that of interstellar medium objects (similar to 1.00) and several aqueously altered carbonaceous chondrites (similar to 1.40). This suggests organics of similar length/branching, and perhaps similar formation regions, for NWA 852, Wild 2 dust particles, and interplanetary dust particles. The heterogeneous spatial distribution of ratio values indicates the presence of a mixture of aliphatic organic material with different length/branching, and thus a wide range of parent body processes, which occurred before the considered grain was formed. C1 [Yesiltas, Mehmet; Peale, Robert E.] Univ Cent Florida, Dept Phys, Orlando, FL 32816 USA. [Unger, Miriam; Hirschmugl, Carol J.] Univ Wisconsin, Dept Phys, Milwaukee, WI 53211 USA. [Sedlmair, Julia] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Peale, RE (reprint author), Univ Cent Florida, Dept Phys, Orlando, FL 32816 USA. EM robert.peale@ucf.edu FU Graduate Fellowship Program by the Turkish government [1416]; University of Wisconsin-Madison; University of Wisconsin-Milwaukee; NSF MRI award [0619759]; NSF CHE award [1112433] FX Mr. Yesiltas was primarily supported through Graduate Fellowship Program #1416 by the Turkish government. SRC was primarily funded by the University of Wisconsin-Madison with supplemental support from facility users and the University of Wisconsin-Milwaukee. IRENI beamline's construction and development was supported by NSF MRI award # 0619759, and CJH was funded by NSF CHE award # 1112433. We thank Prof. Daniel Britt and Prof. Humberto Campins for critically reading the manuscript and making a number of valuable suggestions. We are also grateful to associate editor Dr. G. Benedix and anonymous reviewers for constructive comments on this manuscript. NR 70 TC 1 Z9 1 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1086-9379 EI 1945-5100 J9 METEORIT PLANET SCI JI Meteorit. Planet. Sci. PD OCT PY 2015 VL 50 IS 10 BP 1684 EP 1696 DI 10.1111/maps.12498 PG 13 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA CT1JN UT WOS:000362554700002 ER PT J AU Poulose, SM Thangthaeng, N Miller, MG Shukitt-Hale, B AF Poulose, Shibu M. Thangthaeng, Nopporn Miller, Marshall G. Shukitt-Hale, Barbara TI Effects of pterostilbene and resveratrol on brain and behavior SO NEUROCHEMISTRY INTERNATIONAL LA English DT Review DE Pterostilbene; Resveratrol; Polyphenols; Inflammation; Aging; Brain-signaling ID CANCER CHEMOPREVENTIVE AGENT; HEME OXYGENASE-1 EXPRESSION; CORONARY-HEART-DISEASE; OXIDATIVE STRESS; ALZHEIMERS-DISEASE; HEALTHY-VOLUNTEERS; TRANS-RESVERATROL; LIFE-SPAN; CARDIOVASCULAR-DISEASES; INDUCED NEUROTOXICITY AB Age is the greatest universal risk factor for neurodegenerative diseases. During aging, these conditions progress from minor loss of function to major disruptions in daily life, loss of independence and ultimately death. Because approximately 25% of the world population is expected to be older than age 65 by 2050, and no treatments exist to halt or reverse ongoing neurodegeneration, the need for effective prevention Strategies is more pressing that ever before. A growing body of research supports the role of diet in healthy aging, particularly diets rich in bioactive phytochemical compounds. Recently, stilbenes such as resveratrol (3, 5, 4'-trans-trihydroxystilbene) and its analogue, pterostilbene, have gained a significant amount of attention for their potent antioxidant, anti-inflammatory, and anticarcinogenic properties. However, evidence for the beneficial effects of stilbenes on cerebral function is just beginning to emerge. In this review, we summarize the current knowledge on the role of resveratrol and pterostilbene in improving brain health during aging, with specific focus on antioxidant and antiinflammatory signaling and behavioral outcomes. Published by Elsevier Ltd. C1 [Poulose, Shibu M.; Thangthaeng, Nopporn; Miller, Marshall G.; Shukitt-Hale, Barbara] Tufts Univ, USDA, HNRCA, Boston, MA 02111 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, USDA, HNRCA, Neurosci & Aging Lab, 711 Washington St, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov NR 107 TC 8 Z9 8 U1 6 U2 33 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0197-0186 EI 1872-9754 J9 NEUROCHEM INT JI Neurochem. Int. PD OCT PY 2015 VL 89 BP 227 EP 233 DI 10.1016/j.neuint.2015.07.017 PG 7 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA CT2CO UT WOS:000362609800023 PM 26212523 ER PT J AU Akond, M Liu, SM Kantartzi, SK Meksem, K Bellaloui, N Lightfoot, DA Yuan, JZ Wang, DC Anderson, J Kassem, MA AF Akond, Masum Liu, Shiming Kantartzi, Stella K. Meksem, Khalid Bellaloui, Nacer Lightfoot, David A. Yuan, Jiazheng Wang, Dechun Anderson, James Kassem, My Abdelmajid TI A SNP genetic linkage map based on the "Hamilton' by "Spencer' recombinant inbred line population identified QTL for seed isoflavone contents in soybean SO PLANT BREEDING LA English DT Article DE soybean; single nucleotide polymorphism; quantitative trait loci; isoflavone; daidzein; genistein; glycitein ID QUANTITATIVE TRAIT LOCI; GENOME SEQUENCE; GLYCINE-MAX; MARKERS; ENVIRONMENT; RESISTANCE; DISCOVERY; MECHANISM; PROTEIN; OIL AB Soybean is one of the most important crops worldwide for its protein and oil as well as the health beneficial phytoestrogens or isoflavone. This study reports a relatively dense single nucleotide polymorphism (SNP)-based genetic map based on Hamilton' by Spencer' recombinant inbred line population and quantitative trait loci (QTL) for seed isoflavone contents. The genetic map is composed of 1502 SNP markers and covers about 1423.72cM of the soybean genome. Two QTL for seed isoflavone contents have been identified in this population. One major QTL that controlled both daidzein (qDZ1) and total isoflavone contents (qTI1) was found on LG C2 (Chr 6). And a second QTL for glycitein content (qGT1) was identified on the LG G (Chr 18). These two QTL in addition to others identified in soybean could be used in soybean breeding to optimize isoflavone content. This newly assembled soybean linkage map is a useful tool to identify and map QTL for important agronomic traits and enhance the identification of the genes involved in these traits. C1 [Akond, Masum; Kassem, My Abdelmajid] Fayetteville State Univ, Plant Genom & Biotechnol Lab, Dept Biol Sci, Fayetteville, NC 28301 USA. [Liu, Shiming; Kantartzi, Stella K.; Meksem, Khalid; Lightfoot, David A.; Anderson, James] So Illinois Univ, Dept Plant Soil & Agr Syst, Carbondale, IL 62901 USA. [Bellaloui, Nacer] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Yuan, Jiazheng; Wang, Dechun] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Kassem, My Abdelmajid] Amer Univ Ras Al Khaimah, Dept Biotechnol, Ras Al Khaymah, U Arab Emirates. RP Kassem, MA (reprint author), Fayetteville State Univ, Plant Genom & Biotechnol Lab, Dept Biol Sci, Fayetteville, NC 28301 USA. EM mkassem@uncfsu.edu OI Lightfoot, David/0000-0002-5725-4381 FU Department of Defense (DOD) [W911NF-11-1-0178] FX The authors would like to thank the Department of Defense (DOD) for funding this work through the grant# W911NF-11-1-0178 to MAK and SK. Many thanks to Pam Ratcliff and the rest of the undergraduate and graduate students' crew for taking care of the plants in the greenhouse and the field. NR 63 TC 1 Z9 1 U1 4 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD OCT PY 2015 VL 134 IS 5 BP 580 EP 588 DI 10.1111/pbr.12298 PG 9 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA CT0VR UT WOS:000362516100012 ER PT J AU Strausbaugh, CA Neher, O Rearick, E Eujayl, IA AF Strausbaugh, Carl A. Neher, Oliver Rearick, Eugene Eujayl, Imad A. TI Influence of Harvest Timing, Fungicides, and Beet necrotic yellow vein virus on Sugar Beet Storage SO PLANT DISEASE LA English DT Article ID ROOT-ROT; PHYLOGENETIC DIVERSITY; BOTRYTIS-CINEREA; RESISTANCE; DNA; IDENTIFICATION; STORABILITY; RHIZOMANIA; COMPLEX; EVOLUTION AB Root rots in sugar beet storage can lead to multimillion dollar losses because of reduced sucrose recovery. Thus, studies were conducted to establish additional fungicide treatments for sugar beet storage and a greater understanding of the fungi involved in the sugar beet storage rot complex in Idaho. A water control treatment and three fungicides (Mertect [product at 0.065 ml/kg of roots; 42.3% thiabendazole {vol/vol}], Propulse [product at 0.049 ml/kg of roots; 17.4% fluopyram and 17.4% prothioconazole {vol/vol}], and Stadium [product at 0.13 ml/kg of roots; 12.51% azoxystrobin, 12.51% fludioxonil, and 9.76% difenoconozole fvol/volp were investigated for the ability to control fungal rots of sugar beet roots held up to 148 days in storage during the 2012 and 2013 storage seasons. At the end of September into October, roots were harvested weekly for 5 weeks from each of two sugar beet fields in Idaho, treated with the appropriate fungicide, and placed on top of a commercial indoor sugar beet storage pile until early February. Differences (P < 0.0001 to 0.0150) among fungicide treatments were evident. Propulse- and Stadium-treated roots had 84 to 100% less fungal growth versus the control roots, whereas fungal growth on Mertect-treated roots was not different from the control roots in 7 of 12 comparisons for roots harvested each of the first 3 weeks in both years of this study. The Propulse- and Stadium-treated roots also reduced (P < 0.0001 to 0.0146; based on weeks 1, 3, and 4 in 2012 and weeks 1, 3, 4, and 5 in 2013) sucrose loss by 14 to 46% versus the control roots, whereas roots treated with Mertect did not change sucrose loss compared with the control roots in 7 of 10 evaluations. The predominant fungi isolated from symptomatic roots were an Athelia-like sp., Bottytis cinerea, Penicillium spp., and Phoma betae. If Propulse and Stadium are labeled for use on sugar beet in storage, these fungicides should be considered for root rot control in commercial sugar beet storage and on roots held for vernalization for seed production of this biennial plant species. C1 [Strausbaugh, Carl A.; Eujayl, Imad A.] USDA ARS NWISRL, Kimberly, ID 83341 USA. [Neher, Oliver] Amalgamated Sugar Co, Boise, ID 83709 USA. [Rearick, Eugene] Amalgamated Res LLC, Twin Falls, ID 83301 USA. RP Strausbaugh, CA (reprint author), USDA ARS NWISRL, Kimberly, ID 83341 USA. EM carl.strausbaugh@ars.usda.gov OI Strausbaugh, Carl/0000-0001-6773-1375 FU United States Department of Agriculture CRIS [5368-21220-003-00D]; Amalgamated Sugar Co., LLC; Amalgamated Research, LLC; Beet Sugar Development Foundation; Snake River sugar beet growers FX These data support the objectives of the United States Department of Agriculture CRIS project 5368-21220-003-00D. We thank the Amalgamated Sugar Co., LLC, Amalgamated Research, LLC, Beet Sugar Development Foundation, and Snake River sugar beet growers for supporting our research; and J. Reed, D. Kenney, and T. Brown for their technical support. NR 63 TC 0 Z9 0 U1 0 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1296 EP 1309 DI 10.1094/PDIS-10-14-0998-RE PG 14 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500001 ER PT J AU Kielsmeier-Cook, J Danilova, TV Friebe, B Rouse, MN AF Kielsmeier-Cook, Joshua Danilova, Tatiana V. Friebe, Bernd Rouse, Matthew N. TI Resistance to the Ug99 Race Group of Puccinia graminis f. sp tritici in Wheat-Intra/intergeneric Hybrid Derivatives SO PLANT DISEASE LA English DT Article ID STEM RUST RESISTANCE; IN-SITU HYBRIDIZATION; TRANSLOCATIONS CONFERRING RESISTANCE; FUSARIUM HEAD BLIGHT; LEAF RUST; AEGILOPS-TAUSCHII; CYTOGENETIC CHARACTERIZATION; THINOPYRUM-INTERMEDIUM; AGROPYRON CHROMOSOME; PARTIAL AMPHIPLOIDS AB New races of Puccinia graminis f. sp. tritici, the causal agent of stem rust, threaten global wheat production. In particular, races belonging to the Ug99 race group significantly contribute to yield loss in several Afiican nations. Genetic resistance remains the most effective means of controlling this disease. A collection of 546 wheat-intra- and intergeneric hybrids developed by W. J. Sando (United States Department of Agriculture, Beltsville, MD) was screened with eight races of P. graminis f. sp. tritici, including races TTKSK, TTKST, TTTSK, TRTTF, TTTTF TPMKC, RKQQC, and QTHJC. There were 152 accessions resistant to one or more races and 29 accessions resistant to TTKSK, TTKST, and TTTSK. Of the,se 29 accessions, 9 were resistant to all races, 14 had infection type patterns that were indistinguishable from cultivars possessing Sr9h and Sr42, 2 were indistinguishable from accessions with SrTmp, and 4 did not display resistant patterns of accessions with any known Sr gene. Three accessions (604981, 605286, and 611932) characterized cytogenetically were disornic substitution lines, each with a single Thinopynan ponticum chromosome pair. One accession (606057) was a disomic substitution or addition line with two pairs of T ponticum chromosomes. In total, seven accessions are postulated to contain novel stem rust resistance genes. This research indicates the value of extant collections of wheat-intergeneric hybrids as sources of disease resistance genes. C1 [Kielsmeier-Cook, Joshua; Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Danilova, Tatiana V.; Friebe, Bernd] Kansas State Univ, Wheat Genet Resource Ctr, Dept Plant Pathol, Manhattan, KS 66506 USA. [Rouse, Matthew N.] USDA, Cereal Dis Lab, St Paul, MN USA. RP Kielsmeier-Cook, J (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM kiels008@umn.edu RI Rouse, Matthew/G-5474-2011; OI Kielsmeier-Cook, Joshua/0000-0002-7948-4540 FU USDA-ARS National Plant Disease Recovery System; Durable Rust Resistance in Wheat project; Bill and Melinda Gates Foundation; United Kingdom Department for International development FX This research was supported by the USDA-ARS National Plant Disease Recovery System and the Durable Rust Resistance in Wheat project managed by Cornell University, and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International development. We thank X. Cai and X. Zhu of North Dakota State University for their assistance with the cytogenetic analysis of PI 611899, and H. Bockelman (USDA-ARS) for providing seed of the accessions. NR 74 TC 1 Z9 1 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1317 EP 1325 DI 10.1094/PDIS-09-14-0922-RE PG 9 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500003 ER PT J AU Knaus, BJ Fieland, VJ Graham, KA Grunwald, NJ AF Knaus, B. J. Fieland, V. J. Graham, K. A. Gruenwald, N. J. TI Diversity of Foliar Phytophthora Species on Rhododendron in Oregon Nurseries SO PLANT DISEASE LA English DT Article ID SUDDEN OAK DEATH; WOODY ORNAMENTALS; SEQUENCE-ANALYSIS; RAMORUM BLIGHT; NORTH-AMERICA; SP NOV.; IDENTIFICATION; PATHOGEN; SUSCEPTIBILITY; VIBURNUM AB The genus Phytophthora contains some of the most notorious plant pathogens affecting nursery crops. Given the recent emergence of the sudden oak death pathogen Phytophthora ramorum, particularly in association with Rhododendron spp., characterization of Phytophthora communities associated with this host in nursery environments is prudent. Many taxa may present symptoms similar to P. ramorum but we do not necessarily know their identity, frequency, and importance. Here, we present a survey of Phytophthora taxa observed from seven nurseries in the U.S. state of Oregon. Incidence and diversity of Phytophthora communities differed significantly among nurseries and among seasons within nursery. The taxa P. syringae and P. plurivora were widespread and detected at most of the nurseries sampled. Nine other taxa were also detected but were found either in a single nursery or were shared among only a few nurseries. Characterization of the Phytophthora communities present in nurseries is an important step toward understanding the ecology of these organisms as well as an aid to nursery managers in determining what risks may be present when symptomatic plants are observed. This study builds on an increasing literature, which characterizes Phytophthora community structure in nurseries. C1 [Knaus, B. J.; Graham, K. A.; Gruenwald, N. J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97333 USA. [Fieland, V. J.; Gruenwald, N. J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gruenwald, N. J.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crops Res Unit, Corvallis, OR 97333 USA. EM grunwaln@science.oregonstate.edu OI Knaus, Brian/0000-0003-1665-4343; Grunwald, Niklaus/0000-0003-1656-7602 FU USDA-ARS CRIS [5358-22000-039-00D]; USDA-ARS Floriculture Nursery Initiative; USDA ARS NW Center for Nursery Crops; Oregon Department of Agriculture/Oregon Association of Nurseries (ODA-OAN) FX This project was supported by funds from USDA-ARS CRIS Project 5358-22000-039-00D, the USDA-ARS Floriculture Nursery Initiative, the USDA ARS NW Center for Nursery Crops, and the Oregon Department of Agriculture/Oregon Association of Nurseries (ODA-OAN) research programs. We thank K. Fairchild, M. Larsen, C. Press, and K. Bellingham for technical and administrative support. NR 49 TC 1 Z9 1 U1 0 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1326 EP 1332 DI 10.1094/PDIS-09-14-0964-RE PG 7 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500004 ER PT J AU Liu, ZH El-Basyoni, I Kariyawasam, G Zhang, GR Fritz, A Hansen, J Marais, F Friskop, A Chao, SM Akhunov, E Baenziger, PS AF Liu, Zhaohui El-Basyoni, Ibrahim Kariyawasam, Gayan Zhang, Guorong Fritz, Allan Hansen, Jana Marais, Francois Friskop, Andrew Chao, Shiaoman Akhunov, Eduard Baenziger, P. Stephen TI Evaluation and Association Mapping of Resistance to Tan Spot and Stagonospora Nodorum Blotch in Adapted Winter Wheat Germplasm SO PLANT DISEASE LA English DT Article ID PYRENOPHORA-TRITICI-REPENTIS; HOST-SELECTIVE TOXIN; QUANTITATIVE TRAIT LOCI; SEEDLING RESISTANCE; SPRING WHEAT; PTR TOXA; SEPTORIA DISEASES; YIELD COMPONENTS; GENETIC-ANALYSIS; HEXAPLOID WHEAT AB Tan spot and Stagonospora nodorum blotch (SNB), often occurring together, are two economically significant diseases of wheat in the Northern Great Plains of the United States. They are caused by the fungi Pyrenophora tritici-repentis and Parastagonospora nodorum, respectively, both of which produce multiple necrotrophic effectors (NE) to cause disease. In this work, 120 hard red winter wheat (HRWW) cultivars or elite lines, mostly from the United States, were evaluated in the greenhouse for their reactions to the two diseases as well as NE produced by the two pathogens. One P. nodorum isolate (Sn4) and four Pyrenophora tritici-repentis isolates (Pti2, 331-9, DW5, and AR CrossB10) were used separately in the disease evaluations. NE sensitivity evaluation included ToxA, Ptr ToxB, SnTox1, and SnTox3. The numbers of lines that were rated highly resistant to individual isolates ranged from 11 (9%) to 30 (25%) but only six lines (5%) were highly resistant to all isolates, indicating limited sources of:resistance to both diseases in the U.S. adapted HRWW germplasm. Sensitivity to ToxA was identified in 83 (69%) of the lines and significantly correlated with disease caused by Sn4 and Pti2, whereas sensitivity to other NE was present at much lower frequency and had no significant association with disease. As expected, association mapping located ToxA and SnTox3 sensitivity to chromosome arm 5BL and 5BS, respectively. A total of 24 potential quantitative trait loci was identified with -log (P value) > 3.0 on 12 chromosomes, some of which are novel. This work provides valuable information and tools for HRWW production and breeding in the Northern Great Plains. C1 [Liu, Zhaohui; Kariyawasam, Gayan; Hansen, Jana; Friskop, Andrew] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [El-Basyoni, Ibrahim; Baenziger, P. Stephen] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Zhang, Guorong] Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. [Fritz, Allan] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Marais, Francois] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58102 USA. [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Liu, ZH (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. EM zhh.liu@ndsu.edu OI El-Basyoni, Ibrahim/0000-0002-7210-8788 FU National Institute of Food and Agriculture, United States Department of Agriculture (USDA) [ND02224]; USDA-NIFA Triticeae Coordinated Agricultural Project [2011-68002-30029] FX We thank T. L. Friesen and Z. Zhang for critical review of the manuscript. This material is based upon work supported, in part, by the National Institute of Food and Agriculture, United States Department of Agriculture (USDA), under project number ND02224 to Z. Liu, and by the USDA-NIFA Triticeae Coordinated Agricultural Project, 2011-68002-30029 to P. S. Baenziger, E. Alchunov, and S. Chao. The Triticeae Coordinated Agricultural Project developed and shared the SNP molecular marker information on the lines used in this research. NR 52 TC 1 Z9 1 U1 4 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1333 EP 1341 DI 10.1094/PDIS-11-14-1131-RE PG 9 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500005 ER PT J AU Munck, IA Tanguay, P Wehner, J Villani, SM Cox, KD AF Munck, Isabel A. Tanguay, Philippe Wehner, Jennifer Villani, Sara M. Cox, Kerik D. TI Impact of White Pine Blister Rust on Resistant Cultivated Ribes and Neighboring Eastern White Pine in New Hampshire SO PLANT DISEASE LA English DT Article ID BLACK-CURRANT CULTIVARS; CRONARTIUM-RIBICOLA; NORTH-AMERICA; FOREST PESTS; NIGRUM; AECIOSPORES; ERADICATION; MANAGEMENT; VIABILITY; INFECTION AB To determine the impact of white pine blister rust (WPBR) following the recent breakdown of the Cr resistance dominant gene in cultivated Ribes spp., 255 plants of 19 Ribes cultivars and 445 neighboring eastern white pine (Pinus strobus) from 42 sites across New Hampshire were evaluated. Of the 19 Ribes cultivars evaluated, 15 were WPBR resistant, and 4 of these were labeled as black currant (Ribes nigrum) with the Cr gene (Cr Ribes cultivars). Incidence of WPBR ranged from 0 to 88% for WPBR-resistant Ribes cultivars. Mean WPBR severity was 14 and <6% of leaf area for Cr Ribes and partially resistant cultivars, respectively. The presence of Cronartium ribicola was confinned on 17 of the 19 Ribes cultivars screened with polymerase chain reaction analysis and DNA sequencing. Reference accessions of Cr Ribes cultivars from the Canadian Clonal Genebank were successfully infected with C. ribicola inoculum collected in New Hampshire from Cr Ribes cultivars and P. strobus, confirming that the vCr race of C. ribicola that has overcome the Cr resistance dominant gene in cultivated Ribes spp. is present in New Hampshire. The probability of finding pine trees with WPBR was greater for trees neighboring infected Cr Ribes cultivars (0.18) than trees neighboring WPBR-free Ribes cultivars (0.02). Results from this study suggest that the breakdown of Cr-based resistance in Ribes spp. poses a threat to the white pine resource and to cultivated Ribes production. C1 [Munck, Isabel A.] USDA, Forest Serv, Northeastern Area State & Private Forestry, Durham, NH 03824 USA. [Tanguay, Philippe] Laurentian Forestry Ctr, Canadian Forest Serv, Ste Foy, PQ G1V 4C7, Canada. [Wehner, Jennifer] New Hampshire Dept Resources & Econ Dev, New Hampshire Div Forests & Lands, Concord, NH 03302 USA. [Cox, Kerik D.] Cornell Univ, New York State Agr Expt Stn, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14456 USA. RP Munck, IA (reprint author), USDA, Forest Serv, Northeastern Area State & Private Forestry, Durham, NH 03824 USA. EM imunck@fs.fed.us OI Munck, Isabel/0000-0001-6889-9196 FU USDA Forest Service Evaluation Monitoring grant [NE-EM-B-13-01] FX This study was funded by USDA Forest Service Evaluation Monitoring grant NE-EM-B-13-01. We thank K. Lombard, P. Siegert, C. Smith, and K. Gooch for their contribution in the development of this study; D. Schneider for processing samples in 2012; K. Hummer and P. Zambino for providing advice and helpful suggestions; J. Stanovick for being instrumental in developing the study design and statistical analyses; A. Potvin for help with rust inoculations under controlled conditions; E. Jordan for assistance with fieldwork; T. Rawisnki for identifying wild Ribes spp.; J. Bubar and permit holders who allowed access to their properties; and the editor and two anonymous reviewers for their comments and suggestions. NR 50 TC 0 Z9 0 U1 1 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1374 EP 1382 DI 10.1094/PDIS-12-14-1338-RE PG 9 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500011 ER PT J AU Copes, WE Yang, X Hong, CX AF Copes, Warren E. Yang, Xiao Hong, Chuanxue TI Phytophthora Species Recovered From Irrigation Reservoirs in Mississippi and Alabama Nurseries and Pathogenicity of Three New Species SO PLANT DISEASE LA English DT Article ID HIGH-TEMPERATURE TOLERANT; ORNAMENTAL NURSERIES; GENUS PHYTOPHTHORA; NORTH-CAROLINA; UNITED-STATES; 1ST REPORT; WATER; SPP.; CALIFORNIA; RAMORUM AB From a survey for Phytophthora spp. in containment basins at one nursery each in Alabama and Mississippi, eight species and one taxon were recovered, with Phytophthora gonapodyides dominant in cooler months and P. hydropathica in warmer months, accounting for 39.6 and 46.6% overall recovery, respectively. Among the recoveries were P. macilentosa, P. mississippiae, and P. stricta, three new species recently described from a small lake (labeled M4) that serves as a primary water source for irrigation and to feed another irrigation pond (M5) at the Mississippi nursery. Neither of ponds M4 and M5 directly receives runoff from any production area. The three new species were tested for pathogenicity with Catharanthus roseus, Gardenia jasminoides 'August Beauty,' Hydrangea quercifolia 'Semmes Beauty,' Ilex magland 'Oakland,' Pieris japonica 'Mountain Snow,' and Rhododendron x 'Brandi Michele Raley.' None of the three species infected any of the test plants or became established in peat or pine bark growing media. Based on the result of pathogenicity trials as well as the field observation that none of the nine Phytophthora taxa recovered from irrigation reservoirs have caused episodic disease in the nurseries, they appear to not present a high risk to ornamental plants at those nurseries. C1 [Copes, Warren E.] USDA, ARS, Poplarville, MS 39470 USA. [Yang, Xiao; Hong, Chuanxue] Virginia Polytech Inst & State Univ, Agr Res & Extens Ctr, Dept Plant Pathol Physiol & Weed Sci, Virginia Beach, VA 23455 USA. RP Copes, WE (reprint author), USDA, ARS, Poplarville, MS 39470 USA. EM warren.copes@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service, Thad Cochran Southern Horticultural Laboratory, Southern Horticultural Research Unit [6404-21430-001-00D]; USDA/NIFA [2010-51181-21140] FX The research was supported in part through the US Department of Agriculture, Agricultural Research Service, Thad Cochran Southern Horticultural Laboratory, Southern Horticultural Research Unit project number 6404-21430-001-00D and in part by grants from USDA/NIFA (2010-51181-21140). We thank Mrs. Patricia Richardson for her assistance in performing single-stranded complementary polymorphism (SSCP) of some isolates, verifying identification to species using morphological traits, submitting reports to the group, and generating PCR products and submitting them for DNA sequencing. We also thank both nurseries for allowing our survey of their reservoirs and providing on-site assistances. NR 40 TC 3 Z9 3 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1390 EP 1395 DI 10.1094/PDIS-11-14-1197-RE PG 6 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500013 ER PT J AU Gurung, S Short, DPG Hu, XP Sandoya, GV Hayes, RJ Subbarao, KV AF Gurung, Suraj Short, Dylan P. G. Hu, Xiaoping Sandoya, German V. Hayes, Ryan J. Subbarao, Krishna V. TI Screening of Wild and Cultivated Capsicum Germplasm Reveals New Sources of Verticillium Wilt Resistance SO PLANT DISEASE LA English DT Article ID GENETIC DIVERSITY; DAHLIAE; PEPPER; ANNUUM; LETTUCE; TOMATO; SPP.; SOLANACEAE; ACCESSIONS; SELECTION AB Verticillium wilt caused by Verticillium dahliae is an important soilbome disease of pepper (Capsicum species) worldwide. Most commercial pepper cultivars lack resistance to this pathogen. Our objective was to identify resistance to two V. dahliae isolates in wild and cultivated Capsicum accessions from the core collection of the National Plant Germplasm System of the USDA. Screening of 397 Capsicunz accessions against two V. dahliae isolates (Vdca59 and VdCf45) was performed in a greenhouse. Seventy-eight accessions selected from this screen were further evaluated in a follow-up experiment. In total, 21 (26.9%) and 13 (16.6%) Capsicum accessions tested were resistant to Verticillium wilt when inoculated with V. dahliae isolates VdCa59 and VdCf45, respectively. Eight accessions (Grif 9073, PI 281396, PI 289397, PI 438666, PI 439292, PI 439297, PI 555616, and PI 594125) were resistant to Verticillium wilt against both V. dahliae isolates. On the basis of Germplasm Resources Information Network data, two of the Capsicum annuum accessions (Grif 9073 and PI 439297) were also resistant to Phytophthora root rot disease. These sources of multiple disease resistance will be useful to pepper breeding programs. C1 [Gurung, Suraj; Short, Dylan P. G.; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, Salinas, CA 93905 USA. [Hu, Xiaoping] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. [Hu, Xiaoping] Northwest A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China. [Sandoya, German V.] Univ Calif Davis, Genome Ctr, Salinas, CA 93905 USA. [Sandoya, German V.] Univ Calif Davis, Dept Plant Sci, Salinas, CA 93905 USA. [Hayes, Ryan J.] USDA ARS, Salinas, CA 93905 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, Salinas, CA 93905 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU California Pepper Commission FX The authors thank the excellent technical assistance of Judy Hubbard, Mary Orozco, and Rosa Marchebout during this study and the California Pepper Commission for funding portions of this work. We are also grateful to the USDA Pepper Germplasm Repository for providing the germplasm evaluated in this study. NR 40 TC 0 Z9 0 U1 5 U2 23 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1404 EP 1409 DI 10.1094/PDIS-01-15-0113-RE PG 6 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500015 ER PT J AU Alderman, SC Walenta, DL Hamm, PB Martin, RC Dung, J Kosman, E AF Alderman, Stephen C. Walenta, Darrin L. Hamm, Philip B. Martin, Ruth C. Dung, Jeremiah Kosman, Evsey TI Afternoon Ascospore Release in Claviceps purpurea Optimizes Perennial Ryegrass Infection SO PLANT DISEASE LA English DT Article ID KENTUCKY BLUEGRASS; ERGOT; POPULATIONS; DIVERSITY; SUSCEPTIBILITY; INDIVIDUALS; AEROBIOLOGY; RESISTANCE; VIRULENCE; OREGON AB In Kentucky bluegrass (Poa pratensis), Claviceps purpurea, the causal agent of ergot, typically releases ascospores during the early-morning hours, between about midnight and 10:00 A.M., corresponding to time of flowering, when the unfertilized ovaries are most susceptible to infection. During aeromycology studies of C. purpurea in perennial ryegrass (Lolium perenne) in northeastern Oregon during 2008 to 2010 and 2013, a strain of C. purpurea was found that released ascospores in the afternoon, coinciding with flowering in perernrial ryegrass. Under controlled environmental conditions, sclerotia from perennial ryegrass and Kentucky bluegrass released spores in the afternoon and morning, respectively, consistent with tirning of spore release under field conditions. Internal transcribed spacer (ITS) sequences of single sclerotial isolates from Kentucky bluegrass and perennial ryegrass were consistent with C. putpurea, although minor variations in ITS sequences among isolates were noted. Differences between Kentucky bluegrass and perennial ryegrass isolates were observed in random amplified polymorphic DNA. Evidence is provided for adaptation of C. purpurea to perennial ryegrass by means of delayed spore release that coincides with afternoon flowering in perennial ryegrass. C1 [Alderman, Stephen C.; Martin, Ruth C.] USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. [Walenta, Darrin L.] Oregon State Univ, Union Cty Extens Serv, La Grande, OR 97850 USA. [Hamm, Philip B.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Dept Bot & Plant Pathol, Hermiston, OR 97838 USA. [Dung, Jeremiah] Oregon State Univ, Dept Bot & Plant Pathol, Cent Oregon Agr Res Ctr, Madras 97741, Tamil Nadu, India. [Kosman, Evsey] Tel Aviv Univ, Inst Cereal Crops Improvement, IL-69978 Tel Aviv, Israel. RP Alderman, SC (reprint author), USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. EM aldermas@onid.orst.edu NR 25 TC 1 Z9 1 U1 2 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1410 EP 1415 DI 10.1094/PDIS-09-14-0978-RE PG 6 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500016 ER PT J AU Habibi, A Peever, TL Kim, W Chilvers, MI Chen, W Kaiser, WJ Muehlbauer, FJ AF Habibi, A. Peever, T. L. Kim, W. Chilvers, M. I. Chen, W. Kaiser, W. J. Muehlbauer, F. J. TI First Report of Ascochyta Blight of Spotted Locoweed (Astragalus lentiginosus) Caused by Ascochyta sp in Idaho. SO PLANT DISEASE LA English DT News Item C1 [Habibi, A.; Peever, T. L.; Kim, W.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chilvers, M. I.] Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Chen, W.; Kaiser, W. J.; Muehlbauer, F. J.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Habibi, A (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. NR 3 TC 1 Z9 1 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1446 EP 1446 DI 10.1094/PDIS-02-15-0174-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500023 ER PT J AU Kim, YK Saito, S Xiao, CL AF Kim, Y. K. Saito, S. Xiao, C. L. TI Occurrence of Fludioxonil Resistance in Penicillium digitatum from Citrus in California. SO PLANT DISEASE LA English DT News Item ID BASE-LINE SENSITIVITIES C1 [Kim, Y. K.] Pace Int, Wapato, WA 98951 USA. [Saito, S.; Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Kim, YK (reprint author), Pace Int, Wapato, WA 98951 USA. NR 4 TC 1 Z9 1 U1 2 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1447 EP 1448 DI 10.1094/PDIS-02-15-0226-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500027 ER PT J AU Badillo-Vargas, IE Roe, N Funderburk, JE Adkins, S AF Badillo-Vargas, I. E. Roe, N. Funderburk, J. E. Adkins, S. TI First Report of Tomato chlorotic spot virus in Scarlet Eggplant (Solanum aethiopicum) and American Black Nightshade (Solanum americanum) in the United States. SO PLANT DISEASE LA English DT News Item C1 [Badillo-Vargas, I. E.; Funderburk, J. E.] Univ Florida, NFREC, Quincy, FL 32351 USA. [Roe, N.] Farming Syst Res Inc, Boynton Beach, FL 33474 USA. [Adkins, S.] USDA ARS, Ft Pierce, FL 34945 USA. RP Badillo-Vargas, IE (reprint author), Univ Florida, NFREC, Quincy, FL 32351 USA. NR 5 TC 0 Z9 1 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2015 VL 99 IS 10 BP 1450 EP 1451 DI 10.1094/PDIS-04-15-0385-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CS9CK UT WOS:000362386500034 ER PT J AU Munoz-Amatriain, M Lonardi, S Luo, MC Madishetty, K Svensson, JT Moscou, MJ Wanamaker, S Jiang, T Kleinhofs, A Muehlbauer, GJ Wise, RP Stein, N Ma, YQ Rodriguez, E Kudrna, D Bhat, PR Chao, SM Condamine, P Heinen, S Resnik, J Wing, R Witt, HN Alpert, M Beccuti, M Bozdag, S Cordero, F Mirebrahim, H Ounit, R Wu, YH You, F Zheng, J Simkova, H Dolezel, J Grimwood, J Schmutz, J Duma, D Altschmied, L Blake, T Bregitzer, P Cooper, L Dilbirligi, M Falk, A Feiz, L Graner, A Gustafson, P Hayes, PM Lemaux, P Mammadov, J Close, TJ AF Munoz-Amatriain, Maria Lonardi, Stefano Luo, MingCheng Madishetty, Kavitha Svensson, Jan T. Moscou, Matthew J. Wanamaker, Steve Jiang, Tao Kleinhofs, Andris Muehlbauer, Gary J. Wise, Roger P. Stein, Nils Ma, Yaqin Rodriguez, Edmundo Kudrna, Dave Bhat, Prasanna R. Chao, Shiaoman Condamine, Pascal Heinen, Shane Resnik, Josh Wing, Rod Witt, Heather N. Alpert, Matthew Beccuti, Marco Bozdag, Serdar Cordero, Francesca Mirebrahim, Hamid Ounit, Rachid Wu, Yonghui You, Frank Zheng, Jie Simkova, Hana Dolezel, Jaroslav Grimwood, Jane Schmutz, Jeremy Duma, Denisa Altschmied, Lothar Blake, Tom Bregitzer, Phil Cooper, Laurel Dilbirligi, Muharrem Falk, Anders Feiz, Leila Graner, Andreas Gustafson, Perry Hayes, Patrick M. Lemaux, Peggy Mammadov, Jafar Close, Timothy J. TI Sequencing of 15622 gene-bearing BACs clarifies the gene-dense regions of the barley genome SO PLANT JOURNAL LA English DT Article DE Barley; Hordeum vulgare L; BAC sequencing; gene distribution; recombination frequency; synteny; centromere BACs; HarvEST:Barley; Aegilops tauschii ID BACTERIAL ARTIFICIAL CHROMOSOMES; FLOW-SORTED CHROMOSOMES; DE-NOVO ASSEMBLER; PHYSICAL MAP; GRASS GENOMES; SINGLE-CELL; EVOLUTION; WHEAT; RICE; ORGANIZATION AB Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15622 BACs representing the minimal tiling path of 72052 physical-mapped gene-bearing BACs. This generated 1.7Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barley-Ae.tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge about regions of the barley genome that are gene-dense but low recombination is particularly relevant. C1 [Munoz-Amatriain, Maria; Madishetty, Kavitha; Svensson, Jan T.; Moscou, Matthew J.; Wanamaker, Steve; Ma, Yaqin; Rodriguez, Edmundo; Bhat, Prasanna R.; Condamine, Pascal; Resnik, Josh; Close, Timothy J.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Lonardi, Stefano; Jiang, Tao; Alpert, Matthew; Beccuti, Marco; Bozdag, Serdar; Cordero, Francesca; Mirebrahim, Hamid; Ounit, Rachid; Wu, Yonghui; Zheng, Jie; Duma, Denisa] Univ Calif Riverside, Dept Comp Sci, Riverside, CA 92521 USA. [Luo, MingCheng; Ma, Yaqin; Witt, Heather N.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Svensson, Jan T.] Nord Genet Resource Ctr, SE-23053 Alnarp, Sweden. [Moscou, Matthew J.] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England. [Kleinhofs, Andris; Dilbirligi, Muharrem] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Muehlbauer, Gary J.; Heinen, Shane] Univ Minnesota, Dept Plant Biol, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Wise, Roger P.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res, Ames, IA 50011 USA. [Wise, Roger P.] Iowa State Univ, USDA ARS, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Stein, Nils; Altschmied, Lothar; Graner, Andreas] Leibniz Inst Plant Genet & Crop Plant Res IPK, D-06466 Gatersleben, Germany. [Ma, Yaqin] Molefarming Lab USA, Davis, CA 95616 USA. [Rodriguez, Edmundo] Univ Autonoma Agr Antonio Narro, Dept Ciencias Basicas, Saltillo 25315, Coahuila, Mexico. [Kudrna, Dave; Wing, Rod] Univ Arizona, Arizona Genom Inst, Tucson, AZ 85721 USA. [Bhat, Prasanna R.] Monsanto Res Ctr, Bangalore 560092, Karnataka, India. [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Resnik, Josh] Ronald Reagan UCLA Med Ctr, Los Angeles, CA 90095 USA. [Witt, Heather N.] Univ So Calif, Keck Sch Med, Los Angeles, CA 90033 USA. [Alpert, Matthew] Turtle Rock Studios, Lake Forest, CA 92630 USA. [Beccuti, Marco; Cordero, Francesca] Univ Turin, Dept Comp Sci, I-10149 Turin, Italy. [Bozdag, Serdar] Marquette Univ, Dept Math Stat & Comp Sci, Milwaukee, WI 53233 USA. [Wu, Yonghui] Google Inc, Mountain View, CA 94043 USA. [You, Frank] USDA ARS, Albany, CA 94710 USA. [You, Frank] Agr & Agri Food Canada, Morden, MB R6M 1Y5, Canada. [Zheng, Jie] Nanyang Technol Univ, Sch Comp Engn, Singapore 639798, Singapore. [Simkova, Hana; Dolezel, Jaroslav] Ctr Reg Hana Biotechnol & Agr Res, Inst Expt Bot, CZ-77200 Olomouc, Czech Republic. [Grimwood, Jane; Schmutz, Jeremy] Hudson Alpha Genome Sequencing Ctr, DOE Joint Genome Inst, Huntsville, AL 35806 USA. [Grimwood, Jane; Schmutz, Jeremy] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. [Duma, Denisa] Baylor Coll Med, Jan & Dan Duncan Neurol Res Inst, Houston, TX 77030 USA. [Blake, Tom; Feiz, Leila] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Bregitzer, Phil] USDA ARS, Aberdeen, ID 83210 USA. [Cooper, Laurel; Hayes, Patrick M.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Cooper, Laurel] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Dilbirligi, Muharrem] Sci & Technol Res Council Turkey, Int Cooperat Dept, TR-06100 Ankara, Turkey. [Falk, Anders] Swedish Univ Agr Sci, SE-75007 Uppsala, Sweden. [Feiz, Leila] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Gustafson, Perry] Univ Missouri, USDA, Columbia, MO 65211 USA. [Lemaux, Peggy] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Mammadov, Jafar] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Mammadov, Jafar] Dow AgroSci LLC, Indianapolis, IN 46268 USA. RP Close, TJ (reprint author), Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. EM timothy.close@ucr.edu RI Zheng, Jie/C-1356-2011; Moscou, Matthew/D-5266-2011; Schmutz, Jeremy/N-3173-2013; OI Zheng, Jie/0000-0001-6774-9786; Moscou, Matthew/0000-0003-2098-6818; Schmutz, Jeremy/0000-0001-8062-9172; Cordero, Francesca/0000-0002-3143-3330; Ounit, Rachid/0000-0003-1803-261X; Wing, Rod/0000-0001-6633-6226 FU USDA Initiative for Future Agriculture and Food Systems [01-52100-11346]; North American Barley Genome Project [USDA-CSREES 2001-34213-10511]; USDA-CSREES National Research Initiative (NRI) [2002-35300-12548]; NSF Plant Genome Research Program [DBI-0321756]; BarleyCAP [USDA-CSREES-NRI 2006-55606-16722, USDA-AFRI-NIFA 2009-85606-05701]; USDA-AFRI-NIFA [2009-65300-05645]; TriticeaeCAP [USDA-NIFA 2010-15718-10]; NSF-ABI [DBI-1062301]; UC Riverside Agricultural Experiment Station Hatch Project [CA-R-BPS-5306-H]; Office of Science of the US Department of Energy [DE-AC02-05CH11231]; National Program of Sustainability I [LO1204] FX This work was supported by the USDA Initiative for Future Agriculture and Food Systems 01-52100-11346, North American Barley Genome Project (USDA-CSREES 2001-34213-10511), USDA-CSREES National Research Initiative (NRI) 2002-35300-12548, NSF Plant Genome Research Program DBI-0321756, BarleyCAP (USDA-CSREES-NRI 2006-55606-16722 and USDA-AFRI-NIFA 2009-85606-05701), USDA-AFRI-NIFA 2009-65300-05645, TriticeaeCAP (USDA-NIFA 2010-15718-10), NSF-ABI DBI-1062301, and UC Riverside Agricultural Experiment Station Hatch Project CA-R-BPS-5306-H. The work conducted by the US Department of Energy Joint Genome Institute was supported by the Office of Science of the US Department of Energy under Contract No. DE-AC02-05CH11231. H.S and J.D. have been supported by grant award LO1204 from the National Program of Sustainability I. The authors also thank the following individuals: for communications regarding gene-bearing BAC IDs (Nick Collins, Jorge Dubcovsky, Katherine Feuillet, Kulvinder Gill, Yong Gu, David Laurie, Saghai Maroof, Tim Sutton, Pingsha Hu); for BAC-clone identification (Faith Lin, Ginger Mok, Hung Le); for provision of fee-for-service Morex barley research materials (Michael Atkins, Clemson University Genomics Institute), for provision of fee-for-service DNA sequencing (John Weger, UC Riverside Genomics Core Facility), and for other technical assistance (Gianfranco Ciardo, Raymond Fenton, Hung Le, Harkamal Walia). NR 60 TC 8 Z9 8 U1 5 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD OCT PY 2015 VL 84 IS 1 BP 216 EP 227 DI 10.1111/tpj.12959 PG 12 WC Plant Sciences SC Plant Sciences GA CT1LS UT WOS:000362560400016 PM 26252423 ER PT J AU Agga, GE Scott, HM AF Agga, Getahun E. Scott, H. Morgan TI Use of generalized ordered logistic regression for the analysis of multidrug resistance data SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Antimicrobial resistance; Multidrug resistance; Generalized ordered logistic regression; Ordinal logistic regression; E. coli; Pigs ID FECAL ESCHERICHIA-COLI; ANTIMICROBIAL-RESISTANCE; FEEDLOT CATTLE; COPPER SUPPLEMENTATION; ANTIBIOTIC-RESISTANCE; WEANED PIGS; CHLORTETRACYCLINE; HUMANS; MODELS; SUSCEPTIBILITY AB Statistical analysis of antimicrobial resistance data largely focuses on individual antimicrobial's binary outcome (susceptible or resistant). However, bacteria are becoming increasingly multidrug resistant (MDR). Statistical analysis of MDR data is mostly descriptive often with tabular or graphical presentations. Here we report the applicability of generalized ordinal logistic regression model for the analysis of MDR data. A total of 1,152 Escherichia coli, isolated from the feces of weaned pigs experimentally supplemented with chlortetracycline (CTC) and copper, were tested for susceptibilities against 15 antimicrobials and were binary classified into resistant or susceptible. The 15 antimicrobial agents tested were grouped into eight different antimicrobial classes. We defined MDR as the number of antimicrobial classes to which E. coli isolates were resistant ranging from 0 to 8. Proportionality of the odds assumption of the ordinal logistic regression model was violated only for the effect of treatment period (pre-treatment, during-treatment and post-treatment); but not for the effect of CTC or copper supplementation. Subsequently, a partially constrained generalized ordinal logistic model was built that allows for the effect of treatment period to vary while constraining the effects of treatment (CTC and copper supplementation) to be constant across the levels of MDR classes. Copper (Proportional Odds Ratio [Prop OR] = 1.03; 95% CI = 0.73-1.47) and CTC (Prop OR = 1.1; 95% CI = 0.78-1.56) supplementation were not significantly associated with the level of MDR adjusted for the effect of treatment period. MDR generally declined over the trial period. In conclusion, generalized ordered logistic regression can be used for the analysis of ordinal data such as MDR data when the proportionality assumptions for ordered logistic regression are violated. Published by Elsevier B.V. C1 [Agga, Getahun E.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Scott, H. Morgan] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA. RP Agga, GE (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18 D, Clay Ctr, NE 68933 USA. EM Getahun.Agga@ARS.USDA.GOV OI Agga, Getahun/0000-0002-9903-5283 FU National Pork Board (NPB) [10-121]; College of Veterinary Medicine, Kansas State University FX The experimental study of copper and chlortetracycline supplementation in weaned pigs on antimicrobial resistance was funded by National Pork Board (NPB #10-121) and the College of Veterinary Medicine, Kansas State University. NR 24 TC 0 Z9 0 U1 3 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD OCT 1 PY 2015 VL 121 IS 3-4 BP 374 EP 379 DI 10.1016/j.prevetmed.2015.08.015 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CT2DF UT WOS:000362611500022 PM 26342790 ER PT J AU Gao, Y Liu, FZ Wang, K Wang, DJ Gong, X Liu, LJ Richards, CM Henk, AD Volk, GM AF Gao, Yuan Liu, Fengzhi Wang, Kun Wang, Dajiang Gong, Xin Liu, Lijun Richards, Christopher M. Henk, Adam D. Volk, Gayle M. TI Genetic diversity of Malus cultivars and wild relatives in the Chinese National Repository of Apple Germplasm Resources SO TREE GENETICS & GENOMES LA English DT Article DE Genetic resources; Apple; Diversity; Cultivars ID X-DOMESTICA BORKH.; POPULATION-STRUCTURE; COLLECTION; IDENTIFICATION; RESISTANCE; ACCESSIONS; ROBUSTA; HYBRIDS AB The Research Institute of Pomology (IP), Chinese Academy of Agricultural Sciences (CAAS) in Xingcheng, China, maintains hundreds of apple accessions that originated from around the world. We have used 16 microsatellites to assess the diversity and differentiation of 391 accessions within the IP that represent Malus x domestica (from China, Japan, former Soviet Republics, and Western countries) as well as the crop's wild relative species Malus baccata, Malus prunifolia, Malus x robusta, and Malus sieversii. We identified genetic relationships among these eight source categories that suggest that the M. x domestica cultivars from the former Soviet Republics are most closely related to M. sieversii and may represent an independent lineage of domesticated apples distinct from those found in Western Europe and North America. We show that the M. x domestica cultivars from China and Western sources are genetically similar, whereas the cultivars from Japan are distinct. We also describe two accessions of M. x domestica ssp. chinensis landraces that are believed to be over 2000 years old and are more similar to wild species than are most of the M. x domestica cultivars. We show that the wild, landrace, and cultivar accessions within the IP offer novel diversity to apple breeding programs. C1 [Gao, Yuan; Liu, Fengzhi; Wang, Kun; Wang, Dajiang; Gong, Xin; Liu, Lijun] Chinese Acad Agr Sci, Minist Agr, Key Lab Biol & Genet Improvement Hort Crops Germp, Natl Repository Apple Germplasm Resources,Res Ins, Xingcheng 125100, Liaoning, Peoples R China. [Richards, Christopher M.; Henk, Adam D.; Volk, Gayle M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Wang, K (reprint author), Chinese Acad Agr Sci, Minist Agr, Key Lab Biol & Genet Improvement Hort Crops Germp, Natl Repository Apple Germplasm Resources,Res Ins, 98 Xinghai South St, Xingcheng 125100, Liaoning, Peoples R China. EM wangkun5488@163.com FU Core Research Budget of the Non-Profit Governmental Research Institution (ICS, CAAS); Special Scientific Research Fund of Agricultural Public Welfare Profession of China, the Ministry of Finance, P.R. China [092060302-9, 201303093]; USDA [0210-22310-004-50]; China's Ministry of Science and Technology (MOST) [0210-22310-004-50] FX We thank Professor Jia Jizheng for helpful suggestions and arrangements for the experiments performed at the Key Laboratory of Crop Germplasm and Utilization, Ministry of Agriculture, Beijing, P. R. China. This research was funded by Core Research Budget of the Non-Profit Governmental Research Institution (ICS, CAAS) and the Special Scientific Research Fund of Agricultural Public Welfare Profession of China, the Ministry of Finance, P.R. China (No. 092060302-9 and No. 201303093). This project was performed as part of the Bilateral Agreement between USDA and China's Ministry of Science and Technology (MOST) for cooperation on US-China Agricultural Flagship Projects (Project Number 0210-22310-004-50). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 38 TC 1 Z9 1 U1 6 U2 20 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD OCT PY 2015 VL 11 IS 5 AR 106 DI 10.1007/s11295-015-0913-7 PG 9 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CS9SJ UT WOS:000362431600020 ER PT J AU Jung, S Bassett, C Bielenberg, DG Cheng, CH Dardick, C Main, D Meisel, L Slovin, J Troggio, M Schaffer, RJ AF Jung, Sook Bassett, Carole Bielenberg, Douglas G. Cheng, Chun-Huai Dardick, Chris Main, Dorrie Meisel, Lee Slovin, Janet Troggio, Michela Schaffer, Robert J. TI A standard nomenclature for gene designation in the Rosaceae SO TREE GENETICS & GENOMES LA English DT Article DE Rosaceae; Gene naming; Gene database; Gene families ID X DOMESTICA FRUIT; TRANSCRIPTION FACTOR; PRUNUS-PERSICA; APPLE; GENOME; AUXIN AB The plant family Rosaceae is comprised of a highly diverse group of plants, with a wide range of ornamental and agricultural uses throughout the world. Comparative genomic analyses between rosaceous species are increasingly being used to assign putative function. With the wealth of new data, there is a need to standardize nomenclature to ensure uniformity and clarity across research groups. The Rosaceae Gene Name Standardization Committee was formed to develop a naming guideline for genes in Rosaceae family members. This manuscript details the issues involved with naming genes and proposes a common nomenclature system. The Genome Database for Rosaceae (GDR) (www.rosaceae.org) has developed a gene database that will support user-submitted gene names and annotations in addition to the predicted genes from whole genome sequences. GDR also hosts user-curated lists of gene class symbols. To facilitate standardization of gene names and avoid misinterpretation, the committee recommends that the GDR serve as the database of record for the names of Rosaceae genes and that gene names, gene class symbols, and gene annotation be registered in GDR prior to publication in peer reviewed journals. C1 [Jung, Sook; Cheng, Chun-Huai; Main, Dorrie] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Bassett, Carole; Dardick, Chris] USDA ARS, Appalachian Fruit Res Lab, Kearneysville, WV 25430 USA. [Bielenberg, Douglas G.] Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA. [Meisel, Lee] Univ Chile, Inst Nutr & Tecnol Alimentos, Santiago 7830490, Chile. [Slovin, Janet] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Troggio, Michela] Fdn Edmund Mach, Ist Agr San Michele AllAdige, San Michele All Adige, Italy. [Schaffer, Robert J.] Plant & Food Res Ltd, Auckland, New Zealand. [Schaffer, Robert J.] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand. RP Jung, S (reprint author), Washington State Univ, Dept Hort, Pullman, WA 99164 USA. EM sook_jung@wsu.edu; robert.schaffer@plantandfood.co.nz RI Meisel, Lee/J-6755-2012 OI Meisel, Lee/0000-0002-4806-6864 NR 22 TC 2 Z9 2 U1 3 U2 10 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD OCT PY 2015 VL 11 IS 5 AR 108 DI 10.1007/s11295-015-0931-5 PG 7 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CS9SJ UT WOS:000362431600022 ER PT J AU Jordan, DB Braker, JD Wagschal, K Lee, CC Chan, VJ Dubrovska, I Anderson, S Wawrzak, Z AF Jordan, Douglas B. Braker, Jay D. Wagschal, Kurt Lee, Charles C. Chan, Victor J. Dubrovska, Ievgeniia Anderson, Spencer Wawrzak, Zdzislaw TI X-ray Crystal Structure of Divalent Metal-Activated beta-xylosidase, RS223BX SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE GH43 beta-xylosidase; Inverting mechanism; Divalent metal cations; Activation ID SELENOMONAS-RUMINANTIUM; MOLECULAR REPLACEMENT; SPECIFICITY; HYDROLYSIS; BINDING; CALCIUM AB We report the X-ray crystal structure of a glycoside hydrolase family 43 beta-xylosidase, RS223BX, which is strongly activated by the addition of divalent metal cations. The 2.69 structure reveals that the Ca2+ cation is located at the back of the active-site pocket. The Ca2+ is held in the active site by the carboxylate of D85, an "extra" acid residue in comparison to other GH43 active sites. The Ca2+ is in close contact with a histidine imidazole, which in turn is in contact with the catalytic base (D15) thus providing a mechanism for stabilizing the carboxylate anion of the base and achieve metal activation. The active-site pocket is mirrored by an "inactive-site" pocket of unknown function that resides on the opposite side of the monomer. C1 [Jordan, Douglas B.; Braker, Jay D.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Wagschal, Kurt; Lee, Charles C.; Chan, Victor J.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Dubrovska, Ievgeniia] Northwestern Univ, Feinberg Sch Med, Dept Mol Pharmacol & Biol Chem, Chicago, IL 60611 USA. [Anderson, Spencer; Wawrzak, Zdzislaw] Northwestern Univ, Synchrotron Res Ctr, Argonne, IL 60439 USA. RP Jordan, DB (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM douglas.jordan@ars.usda.gov FU United States Department of Agriculture [CRIS 3620-41000-133-00D, CRIS 5325-41000-049-00] FX This work was supported in part by the United States Department of Agriculture CRIS 3620-41000-133-00D (D.B.J. and J.D.B.) and CRIS 5325-41000-049-00 (K.W, C.C.L, and V.J.C). Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 19 TC 1 Z9 1 U1 3 U2 9 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 EI 1559-0291 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD OCT PY 2015 VL 177 IS 3 BP 637 EP 648 DI 10.1007/s12010-015-1767-z PG 12 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CS2HM UT WOS:000361889900005 PM 26201482 ER PT J AU Moore, SJ Madin, B Norman, G Perkins, NR AF Moore, S. J. Madin, B. Norman, G. Perkins, N. R. TI Risk factors for mortality in cattle during live export from Australia by sea SO AUSTRALIAN VETERINARY JOURNAL LA English DT Article DE cattle; live export; mortality ID PNEUMONIA SHIPPING FEVER; FEEDLOT CATTLE; ALBERTA 1985-1988; DISEASE; PATTERN; CALVES AB ObjectiveThis study investigated mortality trends and risk factors for death for cattle exported live from Australia by sea. MethodsMortality data for all voyages from Australia to all destinations between 1995 and 2012 were analysed retrospectively. Daily mortality trends were assessed for 20 long-haul voyages from Australia to the Middle East and to the Russian Federation between 2010 and 2012. ResultsThe overall voyage mortality percentage was 0.17% across the 13 million cattle exported on 6447 voyages. Mortality rates decreased significantly after 2000 and stabilised at low levels from 2003. The mortality rate for voyages to the Middle East and north Africa (0.44%) was significantly higher than for voyages to south-east Europe (0.28%), north-east Asia (0.12%) and south-east Asia (0.09%). Cattle exported from ports in southern Australia carried a higher mortality risk than those exported from northern ports for both long- and short-haul voyages. The daily mortality rate on long-haul voyages peaked at 3-4 weeks post-departure, although there was a smaller peak at 1-2 weeks. ConclusionThe marked reduction in mortality rate since 2000 is related to a number of factors, including industry initiatives, government legislation and market demand, that have resulted in changes to both the selection of cattle for export and the management of cattle prior to and during voyages. Routine collection of animal performance data, combined with NLIS records and use of methods described here, have the potential to contribute to more effective management of mortality risks across the export chain. C1 [Moore, S. J.] Murdoch Univ, Sch Vet & Life Sci, Murdoch, WA 6150, Australia. [Madin, B.; Perkins, N. R.] AusVet Anim Hlth Serv, Toowoomba, Qld, Australia. [Norman, G.] Dept Agr & Food Western Australia, Toowoomba, Qld, Australia. RP Moore, SJ (reprint author), Natl Anim Dis Ctr, Virus & Pr Res Unit, POB 70, Ames, IA 50010 USA. EM Jo.Moore@ARS.USDA.GOV FU Meat & Livestock Australia; LiveCorp [W.LIV.0252] FX Thanks to Bob Vassallo and John Creeper for helping with livestock movement data. Funding for this project was provided by Meat & Livestock Australia and LiveCorp (project W.LIV.0252). NR 38 TC 1 Z9 1 U1 6 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0005-0423 EI 1751-0813 J9 AUST VET J JI Aust. Vet. J. PD OCT PY 2015 VL 93 IS 10 BP 339 EP 348 DI 10.1111/avj.12355 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA CS6CI UT WOS:000362164400020 PM 26303321 ER PT J AU Schmer, MR Jin, VL Wienhold, BJ AF Schmer, Marty R. Jin, Virginia L. Wienhold, Brian J. TI Sub-surface soil carbon changes affects biofuel greenhouse gas emissions SO BIOMASS & BIOENERGY LA English DT Article DE Soil organic carbon; Life-cycle assessment; Corn grain; Corn stover; Switchgrass; Greenhouse gas emissions ID BIOENERGY; SWITCHGRASS; TILLAGE; CORN AB Changes in direct soil organic carbon (SOC) can have a major impact on overall greenhouse gas (GHG) emissions from biofuels when using life-cycle assessment (LCA). Estimated changes in SOC, when accounted for in an LCA, are typically derived from near-surface soil depths (<30 cm). Changes in subsurface soil depths (>30 cm) could have a large positive or negative impact on overall GHG emissions from biofuels that are not always accounted for. Here, we evaluate how sub-surface SOC changes impact biofuel GHG emissions for corn (Zea mays L.) grain, corn stover, and switchgrass (Panicum virgatum L.) using the (Greenhouse Gases, Regulated Emissions, and Energy Use in the Transportation) GREET model. Biofuel GHG emissions showed as much as a 154% difference between using near-surface SOC stocks changes only or when accounting for both near- and sub-surface SOC stock changes. Differences in GHG emissions highlight the importance of accounting for sub-surface SOC changes especially in bioenergy cropping systems with potential for soil C storage to deeper soil depths. Published by Elsevier Ltd. C1 [Schmer, Marty R.; Jin, Virginia L.; Wienhold, Brian J.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. RP Schmer, MR (reprint author), Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Rm 118,Keim Hall UNL East Campus, Lincoln, NE 68583 USA. EM marty.schmer@ars.usda.gov FU Agricultural Research Service GRACEnet project; REAP project FX This publication is based on work supported by the Agricultural Research Service GRACEnet and REAP projects. NR 17 TC 2 Z9 2 U1 1 U2 13 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD OCT PY 2015 VL 81 BP 31 EP 34 DI 10.1016/j.biombioe.2015.05.011 PG 4 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CS5QZ UT WOS:000362134400006 ER PT J AU Pelaez-Samaniego, MR Yadama, V Garcia-Perez, M Lowell, E AF Pelaez-Samaniego, Manuel Raul Yadama, Vikram Garcia-Perez, Manuel Lowell, Eini TI Abundance and characteristics of lignin liquid intermediates in wood (Pinus ponderosa Dougl. ex Laws.) during hot water extraction SO BIOMASS & BIOENERGY LA English DT Article DE Lignin; Hot water extraction; Ponderosa pine (Pinus ponderosa Dougl. ex Laws.); Pretreatment; Wood ID ANALYTICAL PYROLYSIS; SUCCESSIVE EXTRACTION; BIOMASS RECALCITRANCE; ENZYMATIC-HYDROLYSIS; THERMAL-DEGRADATION; ORGANIC-SOLVENTS; STEAM EXPLOSION; KLASON LIGNIN; BATCH REACTOR; KRAFT LIGNIN AB The objective of this research was to investigate the effects of the conditions of hot water extraction (HWE) on abundance, properties, and structure of lignin depolymerization products. HWE of extracted softwood (ponderosa pine) was conducted using temperatures from 140 to 320 degrees C for 90 min. HWE materials were then subjected to a soxhlet extraction process using dichloromethane (DCM). The composition and molecular weight of this DCM-soluble material was conducted using semi-quantitative Py-GC/MS and ESI/MS, which showed that this material is rich in lignin with low molecular weight (<1200 g mol(-1)) and low polydispersity (similar to 1.4). The highest yield of this material (similar to 4.2%) was obtained in material processed at 260 degrees C. Py-GC/MS of the solid fraction after the DCM process showed a progressive reduction of products of the pyrolysis of lignin as the HWE temperature increased. This finding suggests that the lignin content in the solid decreased due in part to migration from the cell walls and middle lamella to the particles surfaces, particularly the inner surface of the cells. SEM and AFM techniques helped to visualize the ability of this DCM-soluble extract to adhere to cellulosic fibers. Therefore, this lignin-rich material presents properties that depend on the conditions of the operation. Results indicate that during HWE it is possible to control the process parameters to limit or increase the amount of DCM-soluble lignin and its properties as required for downstream processes. (c) 2015 Elsevier Ltd. All rights reserved. C1 [Pelaez-Samaniego, Manuel Raul; Garcia-Perez, Manuel] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. [Pelaez-Samaniego, Manuel Raul] Univ Cuenca, Fac Chem Sci, Cuenca, Ecuador. [Yadama, Vikram] Washington State Univ, Dept Civil & Environm Engn, Pullman, WA 99164 USA. [Yadama, Vikram] Washington State Univ, Composite Mat & Engn Ctr, Pullman, WA 99164 USA. [Lowell, Eini] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR USA. RP Pelaez-Samaniego, MR (reprint author), Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. EM m.r.pelaezsamaniego@email.wsu.edu OI Pelaez-Samaniego, Manuel Raul/0000-0002-7618-9474 FU USDA Forest Service Research and Development Woody Biomass, Bioenergy, and Bioproducts Grant Program [09-JV-11261975-028] FX The support of the USDA Forest Service Research and Development Woody Biomass, Bioenergy, and Bioproducts 2009 Grant Program (Agreement No. 09-JV-11261975-028) is acknowledged. M.R.Pelaez-Samaniego acknowledges the Fulbright Faculty Development Program Scholarship. The authors acknowledge Dr. Armando McDonald (University of Idaho) and the Franceschi Microscopy Center (Washington State University) for providing assistance on conducting the ESI/MS and SEM tests, respectively. NR 84 TC 1 Z9 1 U1 5 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD OCT PY 2015 VL 81 BP 117 EP 128 DI 10.1016/j.biombioe.2015.06.012 PG 12 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CS5QZ UT WOS:000362134400016 ER PT J AU Ruttanaprasert, R Jogloy, S Vorasoot, N Kesmala, T Kanwar, RS Holbrook, CC Patanothai, A AF Ruttanaprasert, R. Jogloy, S. Vorasoot, N. Kesmala, T. Kanwar, R. S. Holbrook, C. C. Patanothai, A. TI Root responses of Jerusalem artichoke genotypes to different water regimes SO BIOMASS & BIOENERGY LA English DT Article DE Drought; Available soil water; Helianthus tuberosus L.; Crop adaptation; Drought tolerance; Bioenergy ID HELIANTHUS-TUBEROSUS L.; DROUGHT TOLERANCE; STRESS; YIELD; PERFORMANCE; BIOMASS; GROWTH AB The objective of this study was to determine effects of drought on selected root growth parameters and develop relationships between root parameters and tuber yield for selected Jerusalem artichoke (JA) genotypes. Three water regimes (Field capacity, 50% available soil water (AW) and 25% AW) and five JA varieties (JA 60, JA 125, JA 5, JA 89 and HEL 65) were planted with factorial treatments in a randomized complete block design with four replications. Data on root dry weight (RDW) and root: shoot ratios (RSR) were measured manually. Root diameter (RD), root length (RI), root surface area (RSA) and root volume (RV) were collected at harvest. Drought tolerance indices (DTI) were calculated for all root parameters. Drought reduced all root parameters and DTI but increased RSR in JA 60, JA 125, JA 5, and HEL 65. JA 125 had high values for all root traits and DTI of these traits under drought stress. JA 60 had high DTI of RDW, RD and RSR under mild and severe water stress. JA 5 had high DTI of RDW, RD, RL, RSR and RV under drought conditions. JA 89 and HEL 65 performed well for RDW, RD, RL and low DTI of all root characteristics. DTI for root parameters were positively correlated with tuber dry weight under mild and severe water stress. The JA 5, JA 60 and JA 125 varieties showed high DTI for some root traits, indicating that better root parameters contributed to higher tuber yield under drought stress. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Ruttanaprasert, R.; Jogloy, S.; Vorasoot, N.; Kesmala, T.; Patanothai, A.] Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. [Jogloy, S.] Khon Kaen Univ, Peanut & Jerusalem Artichoke Improvement Funct Fo, Khon Kaen 40002, Thailand. [Kanwar, R. S.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. [Holbrook, C. C.] USDA ARS, Coastal Plain Expt Stn, Tifton, GA 31793 USA. RP Jogloy, S (reprint author), Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. EM sjogloy@gmail.com FU Khon Kaen University; Thailand Research Fund; Royal Golden Jubilee Ph.D. Program [PHD/0026/2551]; Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University; Higher Education Promotion and National Research University Project of Thailand of the Office of Higher Education Commission through the Food and Functional Food Research Cluster of Khon Kaen University; Thailand Research Fund (TRF) Khon Kaen University (KKU); Faculty of Agriculture KKU FX The Royal Golden Jubilee Ph.D. Program (jointly funded by Khon Kaen University and the Thailand Research Fund) (grant no. PHD/0026/2551), the Thailand Research Fund, provided financial supports to this research through the Senior Research Scholar Project of Professor Dr. Sanun Jogloy and the project was partially supported by the Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University. The Higher Education Promotion and National Research University Project of Thailand of the Office of Higher Education Commission were also acknowledged for funding support through the Food and Functional Food Research Cluster of Khon Kaen University. Finally, we would like to thank the Thailand Research Fund (TRF) Khon Kaen University (KKU) and Faculty of Agriculture KKU for providing financial support for training on manuscript preparation. NR 25 TC 0 Z9 0 U1 9 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD OCT PY 2015 VL 81 BP 369 EP 377 DI 10.1016/j.biombioe.2015.07.027 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CS5QZ UT WOS:000362134400046 ER PT J AU Elkasabi, Y Boateng, AA Jackson, MA AF Elkasabi, Yaseen Boateng, Akwasi A. Jackson, Michael A. TI Upgrading of bio-oil distillation bottoms into biorenewable calcined coke SO BIOMASS & BIOENERGY LA English DT Article DE Pyrolysis; Biomass; Bio-oil; Coke; Calcination ID GAS REACTIVE PYROLYSIS; X-RAY-DIFFRACTION; BIODIESEL PRODUCTION; CARBON; DESULFURIZATION; GRAPHITIZATION; EVOLUTION AB Depleting fossil fuel sources necessitate renewable substitutes for petroleum-based co-products. Fast pyrolysis of biomass generates a hydrocarbon liquid ("bio-oil") amenable to distillation and/or hydro-treatment into hydrocarbon blendstocks. Biorefineries must add value through parallel generation of co-products. We demonstrated a straightforward conversion of bio-oil distillate bottoms into calcined coke. The solid residue was subjected to calcination at 1200 degrees C for 1 h under N-2 atmosphere. The dry calcined product contained 96-99% carbon, was free from sulfur (<0.05% mass fraction), and contained a mass fraction of 0.2-1.1% ash. XRD confirmed steady increases in crystallite size with both devolatilization and calcination. FTIR spectroscopy indicated a loss of functional groups after calcination, except two broad peaks representing C-C and C-O. Temperature programmed oxidation (TPO) of the bottoms before and after calcination illustrates an increasing structural order via the increasing temperature(s) necessary to oxidize the samples. SEM images reveal bubbly morphologies similar to the industrially-favored sponge coke. The electrical resistivity of calcined coke samples measured to be < 1.6 m Omega-m, which closely falls in line with specifications for carbon anodes. Due to the aforementioned qualities and biomass origin, biorenewable calcined coke is an improved alternative to petroleum coke and can find application in carbon anodes, steel carburization, and graphite synthesis. Published by Elsevier Ltd. C1 [Elkasabi, Yaseen; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Jackson, Michael A.] ARS, Natl Ctr Agr Utilizat Res, USDA, Renewable Prod Technol Res Unit, Peoria, IL 61604 USA. RP Elkasabi, Y (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM yaseen.elkasabi@ars.usda.gov FU USDA-NIFA-BRDI [2012-10008-20271] FX We would like to acknowledge Craig Einfeldt for providing support work for the fast pyrolysis experiments. Also, we acknowledge Joseph Uknalis for the SEM images, Craig Johnson for x-ray fluorescence measurements (Drexel University), and Ed Wickham and Phoebe Qi for use of the FTIR spectrometer. We also thank John R. Regalbuto and Qiuli Liu (U. of South Carolina) for performing XRD measurements. USDA-NIFA-BRDI grant 2012-10008-20271 is hereby acknowledged. NR 37 TC 6 Z9 6 U1 1 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD OCT PY 2015 VL 81 BP 415 EP 423 DI 10.1016/j.biombioe.2015.07.028 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CS5QZ UT WOS:000362134400049 ER PT J AU Eggleston, G Andrzejewski, B Cole, M Dalley, C Sklanka, S St Cyr, E Chung, YJ Powell, R AF Eggleston, Gillian Andrzejewski, Brett Cole, Marsha Dalley, Caleb Sklanka, Scottie St Cyr, Eldwin Chung, Yoo-Jin Powell, Randall TI Novel storage technologies for raw and clarified syrup biomass feedstocks from sweet sorghum (Sorghum bicolor L. Moench) SO BIOMASS & BIOENERGY LA English DT Article DE Sweet sorghum; Biomass feedstock; Juice clarification; Syrup; Syrup storage; Fungal deterioration ID PILOT-PLANT; CANE JUICE; FERMENTATION; SUGAR; CLARIFICATION; MANUFACTURE; DETERIORATION AB Attention is currently focused on developing sustainable supply chains of sugar biomass feedstocks for new, flexible biorefineries. Fundamental processing needs identified by industry for the large-scale manufacture of biofuels and bioproducts from sweet sorghum (Sorghum bicolor L. Moench) include stabilization and concentration of juice into syrup for long-term storage, year-round supply, efficient transport, and acceptable end-product yields. Pilot plant studies were conducted to evaluate the storage (up to 160 days at similar to 25 degrees C) of raw and clarified syrups from sweet sorghum hybrid and commercial cultivars. Clarified syrups were manufactured after clarification of juice (80 degrees C; 5 ppm polyanionic flocculant) at various target limed pHs (6.1-6.8) followed by vacuum evaporation. All 70 Brix raw syrups were susceptible to microbial deterioration on the surface during storage, and raw syrups were more susceptible than clarified syrups. Surface deterioration was mainly fungal since bacterial growth was inhibited by low water activity. Juice clarification reduced the loss of fermentable sugars during the evaporation stage and, generally, allowed for better storage of syrup up to 80 days. Target clarification pH had a dramatic effect on the storage of clarified 70 Brix syrups with more acidic pHs reducing fungal deterioration. Further studies are now warranted on the post-evaporation pH adjustment of raw and clarified syrups to <6.1 for long-term storage. Inexpensive soy bean oil and candellila wax showed promise as surface sealants to preserve syrups for at least 80 days of storage at similar to 25 degrees C, also warranting further investigation. (C) 2015 Published by Elsevier Ltd. C1 [Eggleston, Gillian; Andrzejewski, Brett; Cole, Marsha; Sklanka, Scottie; St Cyr, Eldwin; Chung, Yoo-Jin] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Dalley, Caleb] USDA ARS, Sugarcane Unit, Houma, LA 70360 USA. [Powell, Randall] Delta BioRenewables LLC, Memphis, TN USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov OI Chung, Yoo Jin/0000-0001-8491-7470 FU United Sorghum Checkoff Program [USCP RN001-14] FX The authors are grateful to the United Sorghum Checkoff Program (USCP RN001-14) for funding this research. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 29 TC 2 Z9 2 U1 3 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD OCT PY 2015 VL 81 BP 424 EP 436 DI 10.1016/j.biombioe.2015.07.013 PG 13 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CS5QZ UT WOS:000362134400050 ER PT J AU Smith, TW Walinga, C Wang, S Kron, P Suda, J Zalapa, J AF Smith, Tyler W. Walinga, Charlotte Wang, Song Kron, Paul Suda, Jan Zalapa, Juan TI Evaluating the relationship between diploid and tetraploid Vaccinium oxycoccos (Ericaceae) in eastern Canada SO BOTANY LA English DT Article DE AFLP; cranberry; flow cytometry; morphometrics; polyploidy; Vaccinium oxycoccos ID NUCLEAR-DNA CONTENT; FLOW-CYTOMETRY; GENETIC DIVERSITY; PLOIDY LEVEL; POLYPLOIDY; PLANTS; CRANBERRY; AUTOPOLYPLOIDY; HYBRIDIZATION; POPULATIONS AB Vaccinium oxycoccos s.l. is a complex of diploid and polyploid plants. The taxonomic relationship between the cytotypes is uncertain, with conflicting treatments in recent revisions. To clarify this situation, we investigated the relationships among ploidy, morphology, and genetic diversity in this group. We collected samples from a 1000 km transect in eastern Canada. We used flow cytometry to determine DNA ploidy, completed a morphometric analysis of flowering stems, and assessed genetic diversity using AFLPs. Diploids only occurred growing in mixed populations with tetraploids. There were statistically significant morphological differences between ploidies; however, tetraploid variation encompasses the diploid range for most characters. AFLP data demonstrate that the tetraploids have undergone genetic divergence since their formation, obscuring whether they are auto -or allo-polyploids. Our results agree with previous work using isozymes, which revealed genetic divergence of diploids and tetraploids in North America; and morphometry, which demonstrated clear distinctions between diploids and tetraploids in Europe. We found that diploids and tetraploids co-occur much more frequently than previously recognized, which may explain the conflicting treatment of this group by North American taxonomists. We recommend recognizing diploids and tetraploids as distinct species. The distribution of the two species in North America suggests two hypotheses regarding the successful establishment of the tetraploid: the tetraploids' success is due to their capacity to exploit novel habitats outside the range of the diploid; or the tetraploid has in fact already out-competed the diploid in large areas of its former range, limiting the diploid to the far north. While we cannot rule out ongoing gene flow between diploids and tetraploids, it is likely a rare phenomenon in this group. C1 [Smith, Tyler W.; Wang, Song] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada. [Walinga, Charlotte] Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada. [Kron, Paul] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Suda, Jan] Charles Univ Prague, Dept Bot, Fac Sci, CZ-12801 Prague, Czech Republic. [Suda, Jan] Acad Sci Czech Republic, Inst Bot, CZ-25243 Pruhonice, Czech Republic. [Zalapa, Juan] Univ Wisconsin, USDA, ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Smith, TW (reprint author), Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, 960 Carling Ave,KW Neatby Bldg, Ottawa, ON K1A 0C6, Canada. EM tyler.smith@agr.gc.ca RI Suda, Jan/D-3369-2013; OI Suda, Jan/0000-0001-8964-9153; Walinga, Charlotte/0000-0002-4329-6943 NR 50 TC 1 Z9 1 U1 4 U2 23 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 1916-2790 EI 1916-2804 J9 BOTANY JI Botany PD OCT PY 2015 VL 93 IS 10 BP 623 EP 636 DI 10.1139/cjb-2014-0223 PG 14 WC Plant Sciences SC Plant Sciences GA CS3VU UT WOS:000362004100001 ER PT J AU Millar, CI Westfall, RD Delany, DL Flint, AL Flint, LE AF Millar, Constance I. Westfall, Robert D. Delany, Diane L. Flint, Alan L. Flint, Lorraine E. TI Recruitment patterns and growth of high-elevation pines in response to climatic variability (1883-2013), in the western Great Basin, USA SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE recruitment; radial growth; dendrochronology; tree line; climate change ID VOLCANIC-NATIONAL-PARK; SIERRA-NEVADA; ALPINE-TREELINE; BRISTLECONE-PINE; RANGE SHIFTS; CALIFORNIA; MOUNTAINS; DYNAMICS; FORESTS; 20TH-CENTURY AB Over the period 1883-2013, recruitment of subalpine limber pine (Pinus flexilis E. James) and Great Basin bristlecone pine (Pinus longaeva D.K. Bailey) above the upper tree line, below the lower tree line, and across middle-elevation forest borders occurred at localized sites across four mountain ranges in the western Great Basin. A synchronous pulse at all ecotones occurred between 1963 and 2000 (limber pine) and between 1955 and 1978 (bristlecone pine) when pines expanded 225 m beyond forest borders. Little recruitment occurred before this interval or in the 21st century. No obvious environmental factors distinguished recruitment locations from nonrecruitment locations. Where their ranges overlap, limber pine has leapfrogged above bristlecone pine by 300 m. Limber pine tree-ring chronologies, developed to compare radial-growth responses with recruitment, showed dominant pulses of increased growth during the same interval as recruitment. Significant climate correlations of growth and recruitment indicated lead and lag effects as much as 6 years and complex relationships with climate variables, corroborating the importance of cumulative climate effects relative to any single year. Water relations were the most important drivers of growth and recruitment and interacted with growing-season minimum and maximum temperatures. These results underscore the importance of studying ecotones at all margins when evaluating conifer response to climate change. C1 [Millar, Constance I.; Westfall, Robert D.; Delany, Diane L.] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Flint, Alan L.; Flint, Lorraine E.] US Geol Survey, Calif Water Sci Ctr, Sacramento, CA 95819 USA. RP Millar, CI (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. EM cmillar@fs.fed.us NR 66 TC 4 Z9 4 U1 7 U2 42 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD OCT PY 2015 VL 45 IS 10 BP 1299 EP 1312 DI 10.1139/cjfr-2015-0025 PG 14 WC Forestry SC Forestry GA CS2NW UT WOS:000361909000007 ER PT J AU Mortlock, M Kuzmin, IV Weyer, J Gilbert, AT Agwanda, B Rupprecht, CE Nel, LH Kearney, T Malekani, JM Markotter, W AF Mortlock, Marinda Kuzmin, Ivan V. Weyer, Jacqueline Gilbert, Amy T. Agwanda, Bernard Rupprecht, Charles E. Nel, Louis H. Kearney, Teresa Malekani, Jean M. Markotter, Wanda TI Novel Paramyxoviruses in Bats from Sub-Saharan Africa, 2007-2012 SO EMERGING INFECTIOUS DISEASES LA English DT Article AB As part of a larger survey for detection of pathogens among wildlife in sub-Saharan Africa conducted during 2007-2012, multiple diverse paramyxovirus sequences were detected in renal tissues of bats. Phylogenetic analysis supports the presence of at least 2 major viral lineages and suggests that paramyxoviruses are strongly associated with several bat genera. C1 [Mortlock, Marinda; Weyer, Jacqueline; Nel, Louis H.; Markotter, Wanda] Univ Pretoria, ZA-0002 Pretoria, South Africa. [Kuzmin, Ivan V.] Univ Texas Med Branch, Galveston, TX 77555 USA. [Weyer, Jacqueline] Natl Inst Communicable Dis, Johannesburg, South Africa. [Gilbert, Amy T.] USDA, Ft Collins, CO USA. [Agwanda, Bernard] Natl Museums Kenya, Nairobi, Kenya. [Rupprecht, Charles E.] LYSSA LLC, Atlanta, GA USA. [Rupprecht, Charles E.] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA. [Kearney, Teresa] Ditsong Natl Museum Nat Hist, Pretoria, South Africa. [Malekani, Jean M.] Univ Kinshasa, Kinshasa, DEM REP CONGO. RP Markotter, W (reprint author), Univ Pretoria, Dept Microbiol & Plant Pathol, New Agr Bldg,Room 9-2,Main Campus,Private Bag x20, ZA-0028 Hatfield, South Africa. EM wanda.markotter@up.ac.za OI Markotter, Wanda/0000-0002-7550-0080 FU National Research Foundation (NRF) of South Africa [78566, ABI3500, 91496, 92524]; Poliomyelitis Research Foundation (PRF) [12/14]; PRF [11/47]; NRF of South Africa [91496]; University of Pretoria FX This work is based on the research supported in part by a number of grants from the National Research Foundation (NRF) of South Africa (grant number 78566, NRF Research Infrastructure Support Programmes [RISP] grant for the ABI3500, and grant numbers 91496 and 92524) and the Poliomyelitis Research Foundation (PRF) (grant no. 12/14). M.M. was supported by funding from the PRF (grant no. 11/47 [MSc]), the NRF of South Africa (grant number 91496), and the postgraduate study abroad bursary program of the University of Pretoria, who funded the research visit to the Centers for Disease Control and Prevention (CDC). Bat sampling from Kenya and Nigeria was supported by the CDC's Global Disease Detection Program. Sample collection from Cameroon and DRC was supported by the US Agency for International Development's Emerging Pandemic Threats program. NR 13 TC 5 Z9 5 U1 0 U2 9 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD OCT PY 2015 VL 21 IS 10 BP 1840 EP 1843 DI 10.3201/eid2110.140368 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CS5ZY UT WOS:000362158000022 PM 26402433 ER PT J AU Fang, QX Ma, L Halvorson, AD Malone, RW Ahuja, LR Del Grosso, SJ Hatfield, JL AF Fang, Q. X. Ma, L. Halvorson, A. D. Malone, R. W. Ahuja, L. R. Del Grosso, S. J. Hatfield, J. L. TI Evaluating four nitrous oxide emission algorithms in response to N rate on an irrigated corn field SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE RZWQM; Daycent; WNMM; NOE; FASSET; Greenhouse gas emission ID GREENHOUSE-GAS EMISSIONS; DOUBLE-CROPPING SYSTEM; NORTH CHINA PLAIN; N2O EMISSIONS; FERTILIZED FIELDS; GENERALIZED-MODEL; WATER MANAGEMENT; TESTING DAYCENT; CARBON-DIOXIDE; ARABLE CROPS AB In this study, the RZWQM2 (Root Zone Water Quality Model) was used to simulate the responses of N2O emissions to different Nitrogen application rates on an irrigated corn field from 2003 to 2006, in eastern Colorado, USA. Four different algorithms from the literature were coupled with RZWQM2 and compared for simulating N2O emissions during nitrification and denitrification processes. The RZWQM2 was first calibrated for corn yield, N uptake, soil water (0-10 cm) and soil Nitrate-N content (0-180 cm) and the simulated daily nitrification and denitrification rates were used to calculate N2O emission using the algorithms from DAYCENT, NOE (Nitrous Oxide Emissions), WNMM (Water and Nitrogen Management Model), and FASSET models. The best N2O emission was simulated when the fraction of N2O release from nitrification was modified by water filled pore space (WFPS) as in the NOE model and the fraction N2O release from denitrification was adopted from the DAYCENT model. Published by Elsevier Ltd. C1 [Fang, Q. X.] Qingdao Agr Univ, Qingdao, Peoples R China. [Ma, L.; Halvorson, A. D.; Ahuja, L. R.; Del Grosso, S. J.] USDA ARS, Ft Collins, CO 80522 USA. [Malone, R. W.; Hatfield, J. L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. RP Ma, L (reprint author), USDA ARS, 2150 Ctr Ave Bldg D,Ste 200, Ft Collins, CO 80522 USA. EM fangqx@igsnrr.ac.cn; Liwang.Ma@ARS.USDA.GOV NR 91 TC 4 Z9 4 U1 5 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD OCT PY 2015 VL 72 BP 56 EP 70 DI 10.1016/j.envsoft.2015.06.005 PG 15 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA CS2MX UT WOS:000361906400006 ER PT J AU Athanasiadis, IN Janssen, S Holzworth, D Thorburn, P Donatelli, M Snow, V Hoogenboom, G White, JW AF Athanasiadis, Ioannis N. Janssen, Sander Holzworth, Dean Thorburn, Peter Donatelli, Marcello Snow, Val Hoogenboom, Gerrit White, Jeffrey W. TI Thematic issue on agricultural systems modelling and software - Part II Preface SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Editorial Material C1 [Athanasiadis, Ioannis N.; Janssen, Sander] WUR, Wageningen, Netherlands. [Holzworth, Dean; Thorburn, Peter] CSIRO, Canberra, ACT, Australia. [Donatelli, Marcello] CREA, Perugia, Italy. [Snow, Val] AgResearch, Wellington, New Zealand. [Hoogenboom, Gerrit] WSU, New York, NY USA. [White, Jeffrey W.] USDA ARS, Washington, DC 20250 USA. RP Athanasiadis, IN (reprint author), WUR, Wageningen, Netherlands. RI Holzworth, Dean/F-8332-2010; Athanasiadis, Ioannis/F-6301-2010 OI Athanasiadis, Ioannis/0000-0003-2764-0078 NR 0 TC 0 Z9 0 U1 4 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD OCT PY 2015 VL 72 BP 274 EP 275 DI 10.1016/j.envsoft.2015.09.004 PG 2 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA CS2MX UT WOS:000361906400024 ER PT J AU Holzworth, DP Snow, V Janssen, S Athanasiadis, IN Donatelli, M Hoogenboom, G White, JW Thorburn, P AF Holzworth, Dean P. Snow, Val Janssen, Sander Athanasiadis, Ioannis N. Donatelli, Marcello Hoogenboom, Gerrit White, Jeffrey W. Thorburn, Peter TI Agricultural production systems modelling and software: Current status and future prospects SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Agricultural modelling; Crop modelling; Model; Software; Reuse ID SIMULATE YIELD RESPONSE; FAO CROP MODEL; DECISION-SUPPORT-SYSTEMS; GREENHOUSE-GAS EMISSIONS; CLIMATE-CHANGE; FARMING SYSTEMS; CHANGING CLIMATE; SOUTHEASTERN USA; REGIONAL-SCALE; SPECIAL ISSUES AB During the past decade, the application of agricultural production systems modelling has rapidly expanded while there has been less emphasis on model improvement. Cropping systems modelling has become agricultural modelling, incorporating new capabilities enabling analyses in the domains of greenhouse gas emissions, soil carbon changes, ecosystem services, environmental performance, food security, pests and disease losses, livestock and pasture production, and climate change mitigation and adaptation. New science has been added to the models to support this broadening application domain, and new consortia of modellers have been formed that span the multiple disciplines. There has not, however, been a significant and sustained focus on software platforms to increase efficiency in agricultural production systems research in the interaction between the software industry and the agricultural modelling community. This paper describes the changing agricultural modelling landscape since 2002, largely from a software perspective, and makes a case for a focussed effort on the software implementations of the major models. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Holzworth, Dean P.; Thorburn, Peter] CSIRO Agr Flagship, Canberra, ACT, Australia. [Snow, Val] AgResearch, Wellington, New Zealand. [Janssen, Sander] Wageningen UR, Alterra, Wageningen, Netherlands. [Athanasiadis, Ioannis N.] Democritus Univ Thrace, GR-67100 Xanthi, Greece. [Donatelli, Marcello] Consiglio Ric Agr, Bologna, Italy. [Hoogenboom, Gerrit] Washington State Univ, Pullman, WA 99164 USA. [White, Jeffrey W.] USDA ARS, Washington, DC 20250 USA. [Athanasiadis, Ioannis N.] Wageningen Univ, Informat Technol Grp, NL-6700 AP Wageningen, Netherlands. RP Holzworth, DP (reprint author), CSIRO Agr Flagship, Canberra, ACT, Australia. EM Dean.Holzworth@csiro.au RI Thorburn, Peter/A-6884-2011; Holzworth, Dean/F-8332-2010; OI Athanasiadis, Ioannis/0000-0003-2764-0078 NR 119 TC 15 Z9 15 U1 14 U2 45 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD OCT PY 2015 VL 72 BP 276 EP 286 DI 10.1016/j.envsoft.2014.12.013 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA CS2MX UT WOS:000361906400025 ER PT J AU McNider, RT Handyside, C Doty, K Ellenburg, WL Cruise, JF Christy, JR Moss, D Sharda, V Hoogenboom, G Caldwell, P AF McNider, R. T. Handyside, C. Doty, K. Ellenburg, W. L. Cruise, J. F. Christy, J. R. Moss, D. Sharda, V. Hoogenboom, G. Caldwell, Peter TI An integrated crop and hydrologic modeling system to estimate hydrologic impacts of crop irrigation demands SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Agriculture; Hydrology; Drought; Crop modeling; Hydrology modeling ID SOUTHEASTERN UNITED-STATES; CERES-MAIZE MODEL; CLIMATE-CHANGE; WATER-RESOURCES; DSSAT MODEL; YIELD; GROWTH; INFORMATION; ENVIRONMENT; SIMULATION AB The present paper discusses a coupled gridded crop modeling and hydrologic modeling system that can examine the benefits of irrigation and costs of irrigation and the coincident impact of the irrigation water withdrawals on surface water hydrology. The system is applied to the Southeastern U.S. The system tools to be discussed include a gridded version (GriDSSAT) of the crop modeling system DSSAT. The irrigation demand from GriDSSAT is coupled to a regional hydrologic model (WaSSI). GriDSSAT and WaSSI are coupled through the USDA NASS CropScape data to provide crop acreages in each watershed. The crop model provides the dynamic irrigation demand which is a function of the weather. The hydrologic model responds to the weather and includes all other anthropogenic competing uses of water. Examples of the system include an analysis of the hydrologic impact of future expansion of irrigation and the real-time impact of short-term drought. (C) 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). C1 [McNider, R. T.; Handyside, C.; Doty, K.; Ellenburg, W. L.; Cruise, J. F.; Christy, J. R.; Moss, D.] Univ Alabama, Ctr Earth Syst Sci, Huntsville, AL 35899 USA. [Sharda, V.; Hoogenboom, G.] Washington State Univ, AgWeatherNet, Prosser, WA 99350 USA. [Caldwell, Peter] US Natl Forest Serv, Coweta, GA USA. RP McNider, RT (reprint author), Univ Alabama, Ctr Earth Syst Sci, Huntsville, AL 35899 USA. EM mcnider@nsstc.uah.edu FU USDA [2011-67004-30334]; NASA [GOM NNX10AP08G] FX Support for this paper was provided in part by USDA Grant # 2011-67004-30334 - Collaborative Research - Migration of Agricultural Production Back to the Southeast Number and NASA Grant # GOM NNX10AP08G - Use of Satellite Data to Improve the Categorization, Delineation and Mitigation of Drought. Views and information presented in this proposal do not necessarily reflect those of the funding agencies. We also would like to acknowledge the comments of an anonymous reviewer that greatly improved the clarity of the manuscript. NR 71 TC 3 Z9 3 U1 5 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD OCT PY 2015 VL 72 BP 341 EP 355 DI 10.1016/j.envsoft.2014.10.009 PG 15 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA CS2MX UT WOS:000361906400030 ER PT J AU Preisler, HK Schweizer, D Cisneros, R Procter, T Ruminski, M Tarnay, L AF Preisler, Haiganoush K. Schweizer, Donald Cisneros, Ricardo Procter, Trent Ruminski, Mark Tarnay, Leland TI A statistical model for determining impact of wildland fires on Particulate Matter (PM2.5) in Central California aided by satellite imagery of smoke SO ENVIRONMENTAL POLLUTION LA English DT Article DE Air pollution standards; Autoregressive model; Exceptional events; NOAA Hazard Mapping System ID SIERRA-NEVADA; UNITED-STATES; AIR-QUALITY; FORECASTING SYSTEM; FOREST-FIRES; VERIFICATION; WILDFIRES; INCREASE; LAND; AREA AB As the climate in California warms and wildfires become larger and more severe, satellite-based observational tools are frequently used for studying impact of those fires on air quality. However little objective work has been done to quantify the skill these satellite observations of smoke plumes have in predicting impacts to PM2.5 concentrations at ground level monitors, especially those monitors used to determine attainment values for air quality under the Clean Air Act. Using PM2.5 monitoring data from a suite of monitors throughout the Central California area, we found a significant, but weak relationship between satellite-observed smoke plumes and PM2.5 concentrations measured at the surface. However, when combined with an autoregressive statistical model that uses weather and seasonal factors to identify thresholds for flagging unusual events at these sites, we found that the presence of smoke plumes could reliably identify periods of wildfire influence with 95% accuracy. Published by Elsevier Ltd. C1 [Preisler, Haiganoush K.] US Forest Serv, USDA, Pacific Southwest Res Stn, WAB, Albany, CA 94706 USA. [Schweizer, Donald] Univ Calif Merced, Environm Syst Grad Grp, Merced, CA 95343 USA. [Cisneros, Ricardo] Univ Calif Merced, Sch Social Sci Humanities & Arts, Merced, CA 95343 USA. [Procter, Trent] US Forest Serv, USDA, Porterville, CA 93257 USA. [Ruminski, Mark] NOAA, NESDIS, Satellite Anal Branch, College Pk, MD 20740 USA. [Tarnay, Leland] US Forest Serv, USDA, Pacific Southwest Res Stn, Truckee, CA 96161 USA. RP Preisler, HK (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, WAB, 800 Buchanan St, Albany, CA 94706 USA. EM hpreisler@fs.fed.us; dschweizer@ucmerced.edu; rcisneros@ucmerced.edu; tprocter@fs.fed.us; mark.ruminski@noaa.gov; ltarnay@nps.gov NR 28 TC 2 Z9 2 U1 3 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD OCT PY 2015 VL 205 BP 340 EP 349 DI 10.1016/j.envpol.2015.06.018 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CS8AM UT WOS:000362308100039 PM 26123723 ER PT J AU Grilli, E Foresti, F Tugnoli, B Fustini, M Zanoni, MG Pasquali, P Callaway, TR Piva, A Alborali, GL AF Grilli, Ester Foresti, Fabio Tugnoli, Benedetta Fustini, Mattia Zanoni, Maria G. Pasquali, Paolo Callaway, Todd R. Piva, Andrea Alborali, Giovanni L. TI Microencapsulated Sorbic Acid and Pure Botanicals Affect Salmonella Typhimurium Shedding in Pigs: A Close-Up Look from Weaning to Slaughter in Controlled and Field Conditions SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID ORGANIC-ACIDS; IN-VITRO; SWINE; COLONIZATION; BROILERS; POULTRY; PIGLETS; BLEND; VIVO AB The aim of this study was to assess the efficacy of a combination of sorbic acid, thymol, and carvacrol in reducing the prevalence and shedding level of Salmonella Typhimurium in pigs either in a controlled challenge environment or in a production setting. In the first study, 24 weaned piglets were separated in 4 isolation units (6 piglets/isolation unit). Each unit received either a basal diet (no treatment) or a microencapsulated mixture of sorbic acid, thymol, and carvacrol at 1, 2, or 5g/kg of feed. After 21 d, pigs were orally challenged with 6 log(10) colony-forming units of Salmonella Typhimurium. Blood samples and feces from rectal ampullae were collected every week. On d56 of the study, pigs were euthanized and necropsied to collect intestinal contents (jejunum through colon) and ileocecal lymph nodes. Samples were analyzed for Salmonella Typhimurium and serological analysis was also conducted. In the second study, an all-in-all-out multisite pig farm that was positive for monophasic Salmonella Typhimurium was followed throughout a production cycle from weaning to slaughter. Pigs received either a basal diet or the basal diet including 5g/kg of the microencapsulated additive. Environmental, fecal, and blood samples were collected monthly, and cecal contents and ileocecal lymph nodes were collected at slaughter to isolate and enumerate Salmonella. The results indicate that the additive at 5g/kg tended to reduce Salmonella fecal prevalence in both a controlled challenge (p=0.07) and in production conditions (p=0.03). Nevertheless, the additive did not reduce the number of pigs seropositive for Salmonella, nor it reduced the Salmonella prevalence at slaughter. The data indicate that these additives are not effective alone but must be used in conjunction with appropriate containment measures at lairage in order to prevent reinfection in pigs and to reduce the number of pigs carrying Salmonella entering the food chain. C1 [Grilli, Ester; Tugnoli, Benedetta; Fustini, Mattia; Piva, Andrea] Univ Bologna, DIMEVET, I-40064 Bologna, Italy. [Zanoni, Maria G.; Alborali, Giovanni L.] Ist Zooprofilatt Emilia Romagna & Lombardia, Brescia, Italy. [Pasquali, Paolo] Ist Super Sanita, SPVSA, I-00161 Rome, Italy. [Callaway, Todd R.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX USA. RP Grilli, E (reprint author), Univ Bologna, DIMEVET, Via Tolara 50, I-40064 Bologna, Italy. EM ester.grilli@unibo.it FU Vetagro SpA FX This research was supported by a grant from Vetagro SpA. NR 25 TC 0 Z9 0 U1 4 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD OCT 1 PY 2015 VL 12 IS 10 BP 813 EP 819 DI 10.1089/fpd.2015.1953 PG 7 WC Food Science & Technology SC Food Science & Technology GA CS7NT UT WOS:000362271000001 PM 26203634 ER PT J AU Sanchez-Ingunza, R Guard, J Morales, CA Icard, AH AF Sanchez-Ingunza, Roxana Guard, Jean Morales, Cesar A. Icard, Alan H. TI Reduction of Salmonella Enteritidis in the Spleens of Hens by Bacterins That Vary in Fimbrial Protein SefD SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID ENTERICA SEROVAR ENTERITIDIS; ACTIVE SURVEILLANCE NETWORK; PHAGE TYPE-4 INFECTION; 10 US SITES; LAYING HENS; IMMUNE-RESPONSE; WILD-TYPE; VACCINE; CHICKENS; LIVE AB The objective of this research was to determine whether variation in the presence of fimbrial protein SefD would impact efficacy of bacterins as measured by recovery of Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) from the spleens of hens. Two bacterins were prepared that varied in SefD content. Also, two adjuvants were tested, namely, water-in-oil and aluminum hydroxide gel (alum). Control groups for both adjuvant preparations included infected nonvaccinated hens and uninfected nonvaccinated hens. At 21 days postinfection, Salmonella Enteritidis was recovered from 69.7%, 53.1%, and 86.0% from the spleens of all hens vaccinated with bacterins lacking SefD, bacterins that included SefD, and infected nonvaccinated control hens, respectively. No Salmonella was recovered from uninfected nonvaccinates. Results from individual trials showed that both bacterins reduced positive spleens, but that the one with SefD was more efficacious. Alum adjuvant had fewer side effects on hens and egg production as compared to water-in-oil. However, adjuvant did not change the relative recovery of Salmonella Enteritidis from spleens. These results suggest that SefD is a promising target antigen for improving the efficacy of immunotherapy in hens, and is intended to reduce Salmonella Enteritidis in the food supply. C1 [Sanchez-Ingunza, Roxana] CEVA Biomune, Lenexa, KS USA. [Guard, Jean; Morales, Cesar A.] ARS, USDA, Athens, GA 30605 USA. [Icard, Alan H.] Grafton Sci Staffing Co, Leawood, KS USA. RP Guard, J (reprint author), ARS, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM jean.guard@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS), under ARS Project [6612-32000-007-00] FX Animal experiments were approved by the Southeast Poultry Research Laboratory Institutional Animal Care and Use Committee (NIH OLAW Assurance number A4298-01). Dr. Mary Pantin-Jackwood and Dr. Henry Stone, U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) in Athens, GA, provided valuable advice on histopathology and adjuvant formulation. Dr. Richard Gast, USDA, National Poultry Research Center, provided a review of this article. Dr. Zaid Abdo, USDA, ARS, provided independent statistical analysis of data. This research was funded by the U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS), under ARS Project Number 6612-32000-007-00, Genetic Analysis of Poultry-Associated Salmonella enterica to Identify and Characterize Properties and Markers Associated with Egg-Borne Transmission of Illness. NR 39 TC 1 Z9 1 U1 1 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD OCT 1 PY 2015 VL 12 IS 10 BP 836 EP 843 DI 10.1089/fpd.2015.1971 PG 8 WC Food Science & Technology SC Food Science & Technology GA CS7NT UT WOS:000362271000004 PM 26218804 ER PT J AU Haley, BJ Sonnier, J Schukken, YH Karns, JS Van Kessel, JAS AF Haley, Bradd J. Sonnier, Jakeitha Schukken, Ynte H. Karns, Jeffrey S. Van Kessel, Jo Ann S. TI Diversity of Listeria monocytogenes Within a US Dairy Herd, 2004-2010 SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID BULK TANK MILK; UNITED-STATES; EPIDEMIC CLONES; OUTBREAK STRAINS; PREDOMINANT; ENVIRONMENT; PREVALENCE; CATTLE; PERIOD; INLA AB Listeria monocytogenes, the causative agent of listeriosis, is frequently isolated from the environment. Dairy cows and dairy farm environments are reservoirs of this pathogen, where fecal shedding contributes to its environmental dispersal and contamination of milk, dairy products, and meat. The molecular diversity of 40 L. monocytogenes isolates representing 3 serogroups (1/2a, 1/2b, and 4b) collected between 2004 and 2010 from the feces of dairy cattle on a single dairy farm was assessed using a multivirulence locus sequence typing (MVLST) assay. The dairy farm L. monocytogenes MVLST patterns were compared to those from 138 strains isolated globally from clinical cases, foods, and the environment. Results of the study demonstrated that several distantly related L. monocytogenes strains persisted among members of the herd over the course of the study while other strains were transient. Furthermore, some strains isolated during this study appear to be distantly related to previously isolated L. monocytogenes while others are closely related to Epidemic Clones associated with human illness. This work demonstrates that dairy cows can be reservoirs of a diverse population of potentially human pathogenic L. monocytogenes that represents a risk to consumers of milk, dairy products, and meat. C1 [Haley, Bradd J.; Sonnier, Jakeitha; Karns, Jeffrey S.; Van Kessel, Jo Ann S.] ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Schukken, Ynte H.] Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY USA. RP Van Kessel, JAS (reprint author), ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, USDA, Room 202,Bldg 173 BARC East, Beltsville, MD 20705 USA. EM joann.vankessel@ars.usda.gov OI Schukken, Ynte/0000-0002-8250-4194 NR 30 TC 1 Z9 3 U1 0 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD OCT 1 PY 2015 VL 12 IS 10 BP 844 EP 850 DI 10.1089/fpd.2014.1886 PG 7 WC Food Science & Technology SC Food Science & Technology GA CS7NT UT WOS:000362271000005 PM 26325149 ER PT J AU Zhang, JW Young, DH Luckow, KR AF Zhang, Jianwei Young, David H. Luckow, Kenneth R. TI Effect of redistributing windrowed topsoil on growth and development of ponderosa pine plantations SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Windrowing site preparation; Soil nitrogen displacement; Soil respreading; Nitrogen mineralization; Pinus ponderosa; Plantation growth ID SITE PREPARATION; SOIL; DISPLACEMENT; PIEDMONT; REMOVAL; FOREST AB Windrowing site preparation often displaces significant amounts of topsoil including nutrients and carbon into the strip-piles. Although short-term growth may increase due to the early control of competing vegetation, this practice can reduce long-term plantation productivity. Here, we report an experiment established in 1989 in a 28-year-old ponderosa pine (Pinus ponderosa) plantation to determine if redistributing topsoil, along with several shrub control measures, have influenced soil fertility and tree growth. Five treatments from a partial factorial design with three levels of shrub treatment and two levels of soil manipulation were applied in each of five blocks and consisted of: Control (C, do nothing); understory hydroaxed (masticated) to chips and left in the plot (H); windrows redistributed over brush (S); understory hydroaxed and windrows redistributed over chips (SH); and understory manually removed off-site and windrows redistributed (SM). Over the next 21 year period total windrowed topsoil volume and mass were determined, soil nutrient concentrations in and between windrows including soil mineralizable N, total N and C were determined, understory biomass measured, tree diameter, basal area, and volume measured in 1989, 1994, 2005 and 2010, and nitrogen concentration of tree foliage was measured in 1989, 1991 and 1994. Results showed that about 18 cm of topsoil had been displaced into windrows, including 1.98 (+/- 0.13) Mg N ha(-1) and 41.04 (+/- 2.46) Mg carbon ha(-1). In general, redistributing windrowed topsoil (S, SH, and SM) yielded a consistently positive effect on quadratic mean diameter, basal area (BA), and volume compared to C and H. No difference in growth was found between SH and SM. These results were supported by higher soil nitrogen and mineralizable nitrogen contents in the three topsoil redistribution treatments. Higher foliage nitrogen concentrations in the redistribution treatments further supported these higher tree growth rates. The positive effects of shrub removal were evidenced only on the treatments without topsoil redistribution (C versus H); the difference in BA and volume between C and H was only significant in 1994. Redistributing topsoil reduced woody plant biomass but significantly enhanced herbaceous biomass six years after treatment. This shows that windrowing site preparation reduces plantation growth and stand development through displacement of topsoil and its nutrients. These negative effects can be mitigated by carefully redistributing windrowed topsoil, even in an established plantation. Published by Elsevier B.V. C1 [Zhang, Jianwei] US Forest Serv, Pacific Southwest Res Stn, USDA, Redding, CA 96002 USA. [Young, David H.] US Forest Serv, Shasta Trinity Natl Forest, USDA, Redding, CA 96002 USA. [Luckow, Kenneth R.] US Forest Serv, Ouachita Natl Forest, USDA, Hot Springs, AR 71901 USA. RP Zhang, JW (reprint author), US Forest Serv, Pacific Southwest Res Stn, USDA, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM jianweizhang@fs.fed.us NR 27 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 1 PY 2015 VL 353 BP 148 EP 155 DI 10.1016/j.foreco.2015.05.039 PG 8 WC Forestry SC Forestry GA CS4PQ UT WOS:000362058700016 ER PT J AU Baker, SC Halpern, CB Wardlaw, TJ Crawford, RL Bigley, RE Edgar, GJ Evans, SA Franklin, JF Jordan, GJ Karpievitch, Y Spies, TA Thomson, RJ AF Baker, Susan C. Halpern, Charles B. Wardlaw, Timothy J. Crawford, Rodney L. Bigley, Richard E. Edgar, Graham J. Evans, Shelley A. Franklin, Jerry F. Jordan, Gregory J. Karpievitch, Yuliya Spies, Thomas A. Thomson, Russell J. TI Short- and long-term benefits for forest biodiversity of retaining unlogged patches in harvested areas SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Edge effects; Forest influence; Retention forestry; Variable retention; Plants; Invertebrates ID GROUND-DWELLING SPIDERS; GREEN-TREE RETENTION; VARIABLE-RETENTION; INVERTEBRATE ASSEMBLAGES; PACIFIC-NORTHWEST; VANCOUVER-ISLAND; BOREAL FOREST; RESPONSES; EDGE; CONSERVATION AB Aggregated retention, in which patches of trees (aggregates) remain unlogged within larger harvested units, was first applied commercially in 1986. A primary goal was to maintain greater diversity of forest-dependent species through harvest, relative to conventional clearcutting. Despite its global application, the long-term benefits for biodiversity and the comparative responses of disparate taxonomic groups to aggregated retention are largely unknown. A critical knowledge gap relates to the role of 'forest influence' whether and to what extent aggregates affect biodiversity in neighboring harvested areas. We sampled plants, beetles, and spiders/harvestmen in the world's three oldest aggregated-retention sites (21-26 years old), matched with three recently harvested sites (5-8 years old). For each taxonomic group, we compared species composition between undisturbed aggregates and regenerating forests to assess the 'lifeboating' function of aggregates. For each group, we also modeled changes in species composition, and in the numbers of aggregate- and regeneration-affiliated species, with distance from the aggregate edge into the regenerating forest along transects at north-facing edges. For all three taxa, species composition differed between aggregates and regenerating forests in both older and recent sites, confirming the long-term effectiveness of aggregates for lifeboating. The compositional difference between habitats was significantly greater at recent than at older sites for plants, but not for invertebrates. Plants and spiders/harvestmen responded to forest influence, with a marginal response for beetles. Responses for plants and spiders generally manifested as increased numbers of aggregate-affiliated species and decreased numbers of regeneration-affiliated species in regenerating areas closer to edges. Our results indicate that aggregated retention has short- and long-term benefits for biodiversity reflecting both the lifeboating and forest-influence functions of aggregates. However, variation in the responses of plants, beetles and spiders suggests that these benefits cannot be generalized among taxa. We advocate broader application of aggregated retention in forests managed for timber production and encourage managers to incorporate the benefits of forest influence in harvest designs by arranging aggregates to reduce average distances from harvested areas to unlogged habitats. (C) 2015 Elsevier B.V. All rights reserved. C1 [Baker, Susan C.; Wardlaw, Timothy J.; Evans, Shelley A.; Jordan, Gregory J.] Univ Tasmania, Sch Biol Sci, Hobart, Tas 7001, Australia. [Baker, Susan C.; Wardlaw, Timothy J.] Forestry Tasmania, Hobart, Tas 7001, Australia. [Baker, Susan C.; Halpern, Charles B.; Evans, Shelley A.; Franklin, Jerry F.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Crawford, Rodney L.] Univ Washington, Burke Museum, Seattle, WA 98195 USA. [Bigley, Richard E.] Washington State Dept Nat Resources, Forest Resources Div, Olympia, WA 98505 USA. [Edgar, Graham J.; Thomson, Russell J.] Univ Tasmania, Inst Marine & Antarctic Studies, Hobart, Tas 7001, Australia. [Karpievitch, Yuliya] Univ Tasmania, Sch Math & Phys, Hobart, Tas 7001, Australia. [Spies, Thomas A.] US Forest Serv, USDA, PNW Res Stn, Corvallis, OR 97331 USA. RP Baker, SC (reprint author), Forestry Tasmania, GPO Box 207, Hobart, Tas 7001, Australia. EM sue.baker@forestrytas.com.au RI Thomson, Russell/H-5653-2012; OI Thomson, Russell/0000-0003-4949-4120; Jordan, Greg/0000-0002-6033-2766 FU Australian Research Council Linkage grant [LP100100050]; Forestry Tasmania; Fulbright Scholarship - Washington Department of Natural Resources (DNR); University of Washington FX This research was funded by the Australian Research Council Linkage grant LP100100050, Forestry Tasmania, and a Fulbright Scholarship awarded to S.C.B., sponsored by Washington Department of Natural Resources (DNR) and the University of Washington. The study design benefited from input from a group of 16 forest ecologists and managers at a retention forestry workshop in Sweden in 2011 along with US Forest Service statistician Ashley Steel. Our research at the Big Dog site in Mt. Rainier National Park was conducted under permit MORA-2012-SCI-0027. A fantastic field crew assisted with plant surveys: Alessandra Zuin, Kara Randall, Paul Yurky, and Lauren Urgenson. Dana Ross and Paul Yurky assisted with processing of beetles and Chris Looney, Rod Rood, Samuel Perry, and Paul Hammond assisted with beetle identification. Numerous DNR staff under the leadership of Angus Brodie facilitated the project, as did Kirk Naylor from PacifiCorp. Lorin Hicks, Stu Farber and Tim Halme provided information on sites harvested by Plum Creek. We thank Sophie Edgar for producing the map of study site locations. We are grateful to the anonymous referees for useful suggestions on previous versions of this paper. NR 54 TC 9 Z9 9 U1 5 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 1 PY 2015 VL 353 BP 187 EP 195 DI 10.1016/j.foreco.2015.05.021 PG 9 WC Forestry SC Forestry GA CS4PQ UT WOS:000362058700020 ER PT J AU Dudley, MM Burns, KS Jacobi, WR AF Dudley, M. M. Burns, K. S. Jacobi, W. R. TI Aspen mortality in the Colorado and southern Wyoming Rocky Mountains: Extent, severity, and causal factors SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Aspen; Forest health; Dieback; Cytospora; Regeneration ID FOREST DIE-OFF; POPULUS-TREMULOIDES; NATIONAL-PARK; SOUTHWESTERN COLORADO; HYDRAULIC CONDUCTANCE; USA; DROUGHT; DIEBACK; REGENERATION; INSECTS AB Aspen mortality and overstory dieback have occurred at unusually high levels throughout Colorado and western North America over the past fifteen years. Findings from southwestern Colorado indicated dieback and mortality was clearly related to climate stress, coupled with disease and insect attack. To determine whether similar amounts and causes of mortality were occurring in other mountainous regions of Colorado and southern Wyoming, we determined the health status of aspen on five national forests during 2009-2010. We established 500 random characterization plots in aspen-dominated stands on four of the five national forests and 97 detailed measurement plots, with half in 'damaged' and half in 'healthy' aspen stands (as defined by USDA Forest Service aerial detection surveys). Overstory aspen was healthy overall, in spite of nearly ubiquitous presence of disease among plots (99%) and high incidence of insect damage (50-75%); in detailed plots standing adult dead aspen percentages ranged from 20% to 33% among the five national forests. Adult aspen in damaged stands occurred at lower densities, and had thinner crowns (41% dead crown, compared to 16% in healthy stands). Damaged stands also had more standing dead adult trees (37%) than in healthy stands (16%). The proportion of live sapling stems and density of saplings did not differ between healthy and damaged stands. Incidence of select mortality agents, including wood-boring insects (Agrilus liragus and Saperda calcarata) (40% in damaged, 18% in healthy), aspen bark beetles (Procryphalus mucronatus and Dypophloeus populi) (31% in damaged, 12% in healthy) and Cytospora canker (caused by fungi in the genus Cytospora) (40% in damaged, 18% in healthy) was considerably greater among damaged stands. Aspen bark beetles were more common on south-facing sites than east or west-facing sites (27%, 17%, and 15%, respectively); Cytospora canker was less common on sites above 2800 m (23%) than on lower-elevation sites (28-34%). Prevalence of wood borers, aspen bark beetles and Cytospora canker were all significantly and negatively correlated with latitude among at least two of three aspen size classes. Attempts to model various measures of health of adult aspen (i.e. crown dieback and stem mortality) using site and climatic variables were largely unsuccessful. Since no differences were detected in density of regeneration between damaged or healthy stands, we conclude that most stands will persist upon the landscape for the foreseeable future. Further, we note that although observed densities of regeneration in damaged stands are not as great as levels observed following complete stand removal, suckering does occur, and stems are measurably healthier than regeneration in healthy stands. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dudley, M. M.; Jacobi, W. R.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Burns, K. S.] US Forest Serv, USDA, Forest Hlth Protect, Lakewood Serv Ctr, Golden, CO 80401 USA. RP Dudley, MM (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM meg.dudley@rams.colostate.edu; ksburns@fs.fed.us; william.jacobi@colostate.edu FU USDA Forest Service, Forest Health Protection, Evaluation and Monitoring Program FX We would like to acknowledge and thank the following individuals: James ZumBrunnen, Greybill Statistical Laboratory, Colorado State University; James Worrall, USDA Forest Service; James Blodgett, USDA Forest Service; Brian Howell, USDA Forest Service; Wayne Sheppard, (Ret.) Colorado State University; and Scott Nilson, Margaret Hollowed, Mariah Coler, Lea Pace, Jillian Portillo, and Danielle Turley for field and laboratory assistance. This study was funded by a grant from the USDA Forest Service, Forest Health Protection, Evaluation and Monitoring Program. NR 54 TC 2 Z9 2 U1 3 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 1 PY 2015 VL 353 BP 240 EP 259 DI 10.1016/j.foreco.2015.06.002 PG 20 WC Forestry SC Forestry GA CS4PQ UT WOS:000362058700025 ER PT J AU Sun, SL Sun, G Caldwell, P McNulty, SG Cohen, E Xiao, JF Zhang, Y AF Sun, Shanlei Sun, Ge Caldwell, Peter McNulty, Steven G. Cohen, Erika Xiao, Jingfeng Zhang, Yang TI Drought impacts on ecosystem functions of the US National Forests and Grasslands: Part I evaluation of a water and carbon balance model SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Evapotranspiration; Gross primary productivity; National Forests and Grasslands; Water yield; Modeling ID NET PRIMARY PRODUCTIVITY; WESTERN UNITED-STATES; NORTH-AMERICA; EVAPOTRANSPIRATION ALGORITHM; VEGETATION INDEX; SATELLITE DATA; MODIS; CONSEQUENCES; INVERSION; EXCHANGE AB Understanding and quantitatively evaluating the regional impacts of climate change and variability (e.g., droughts) on forest ecosystem functions (i.e., water yield, evapotranspiration, and productivity) and services (e.g., fresh water supply and carbon sequestration) is of great importance for developing climate change adaptation strategies for National Forests and Grasslands (NFs) in the United States. However, few reliable continental-scale modeling tools are available to account for both water and carbon dynamics. The objective of this study was to test a monthly water and carbon balance model, the Water Supply Stress Index (WaSSI) model, for potential application in addressing the influences of drought on NFs ecosystem services across the conterminous United States (CONUS). The performance of the WaSSI model was comprehensively assessed with measured streamflow (Q) at 72 U.S. Geological Survey (USGS) gauging stations, and satellite-based estimates of watershed evapotranspiration (ET) and gross primary productivity (GPP) for 170 National Forest and Grassland (NFs). Across the 72 USGS watersheds, the WaSSI model generally captured the spatial variability of multi-year mean annual and monthly Q and annual ET as evaluated by Correlation Coefficient (R = 0.71-1.0), Nash Sutcliffe Efficiency (NS = 0.31-1.00), and normalized Root Mean Squared Error (0.06-0.48). The modeled ET and GPP by WaSSI agreed well with the remote sensing-based estimates for multi-year annual and monthly means for all the NFs. However, there were systemic discrepancies in GPP between our simulations and the satellite-based estimates on a yearly and monthly scale, suggesting uncertainties in GPP estimates in all methods (i.e., remote sensing and modeling). Overall, our assessments suggested that the WaSSI model had the capability to reconstruct the long-term forest watershed water and carbon balances at a broad scale. This model evaluation study provides a foundation for model applications in understanding the impacts of climate change and variability (e.g., droughts) on NFs ecosystem service functions. Published by Elsevier B.V. C1 [Sun, Shanlei; Zhang, Yang] N Carolina State Univ, Dept Marine Earth & Atmospher Sci, Raleigh, NC 27606 USA. [Sun, Ge; McNulty, Steven G.; Cohen, Erika] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Caldwell, Peter] USDA FS, Coweeta Hydrol Lab, Southern Res Stn, Otto, NC 28763 USA. [Xiao, Jingfeng] Univ New Hampshire, Inst Study Earth Oceans & Space, Earth Syst Res Ctr, Durham, NH 03824 USA. RP Sun, G (reprint author), US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, 920 Main Campus Dr,Venture 2,Suite 300, Raleigh, NC 27606 USA. EM gesun@fs.fed.us FU National Science Foundation EaSM program [AGS-1049200]; Eastern Forest Environment Threat Assessment Center (EFETAC), USDA Forest Service; NASA Carbon Cycle Science Program [NNX14AJ18G]; NASA Terrestrial Ecology Program [NNX12AK56G] FX This study was funded by the National Science Foundation EaSM program (AGS-1049200) awarded to North Carolina State University, and the Eastern Forest Environment Threat Assessment Center (EFETAC), USDA Forest Service. J. Xiao was supported by NASA's Carbon Cycle Science Program (grant # NNX14AJ18G) and Terrestrial Ecology Program (grant # NNX12AK56G). NR 62 TC 6 Z9 6 U1 9 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 1 PY 2015 VL 353 BP 260 EP 268 DI 10.1016/j.foreco.2015.03.054 PG 9 WC Forestry SC Forestry GA CS4PQ UT WOS:000362058700026 ER PT J AU Sun, SL Sun, G Caldwell, P McNulty, S Cohen, E Xiao, JF Zhang, Y AF Sun, Shanlei Sun, Ge Caldwell, Peter McNulty, Steve Cohen, Erika Xiao, Jingfeng Zhang, Yang TI Drought impacts on ecosystem functions of the US National Forests and Grasslands: Part II assessment results and management implications SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Droughts; Ecosystem productivity; National Forests and Grasslands; Water yield ID WESTERN UNITED-STATES; CLIMATE-CHANGE; NORTH-AMERICA; TEMPORAL VARIABILITY; PRIMARY PRODUCTIVITY; DIE-OFF; WATER; DISTURBANCE; VEGETATION; CONSEQUENCES AB The 781,000 km(2) (193 million acre) United States National Forests and Grasslands system (NF) provides important ecosystem services such as clean water supply, timber production, wildlife habitat, and recreation opportunities to the American public. Quantifying the historical impacts of climate change and drought on ecosystem functions at the national scale is essential to develop sound forest management and watershed restoration plans under a changing climate. This study applied the previously validated Water Supply and Stress Index model (WaSSI) to 170 NFs in the conterminous U.S. (CONUS) to examine how historical extreme droughts have affected forest water yield (Q) and gross primary productivity (GPP). For each NF, we focused on the five years with the lowest annual SPI3 (Standardized Precipitation Index on a 3-month time scale) during 1962-2012. The extent of extreme droughts as measured by the number of NFs and total area affected by droughts has increased during the last decade. Across all lands in CONUS, the most extreme drought during the past decade occurred in 2002, resulting in a mean reduction of Q by 32% and GPP by 20%. For the 170 individual NFs, on average, the top-five droughts represented a reduction in precipitation by 145 mm yr(-1) (or 22%), causing reductions in evapotranspiration by 29 mm yr(-1) (or 8%), Q by 110 mm yr(-1) (or 37%) and GPP by 65 gC m(2) yr(-1) (or 9%). The responses of the forest hydrology and productivity to the top-five droughts varied spatially due to different land-surface characteristics (e.g., climatology and vegetation) and drought severity at each NF. This study provides a comprehensive benchmark assessment of likely drought impacts on the hydrology and productivity in NFs using consistent methods and datasets across the conterminous U.S. The study results are useful to the forestry decision makers for developing appropriate strategies to restore and protect ecosystem services in anticipating potential future droughts and climate change. Published by Elsevier B.V. C1 [Sun, Shanlei] Nanjing Univ Informat Sci & Technol, Coll Hydrometeorol, Nanjing 210044, Jiangsu, Peoples R China. [Sun, Shanlei; Zhang, Yang] N Carolina State Univ, Dept Marine Earth & Atmospher Sci, Raleigh, NC 27606 USA. [Sun, Ge; McNulty, Steve; Cohen, Erika] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Caldwell, Peter] USDA FS, Coweeta Hydrol Lab, Southern Res Stn, Otto, NC 28763 USA. [Xiao, Jingfeng] Univ New Hampshire, Inst Study Earth Oceans & Space, Earth Syst Res Ctr, Durham, NH 03824 USA. RP Sun, G (reprint author), US Forest Serv, USDA, 920 Main Campus Dr,Venture 2,Suite 300, Raleigh, NC 27606 USA. EM gesun@fs.fed.us FU National Science Foundation EaSM program [AGS-1049200]; Eastern Forest Environment Threat Assessment Center (EFETAC), USDA Forest Service; NASA Carbon Cycle Science Program [NNX14AJ18G]; NASA Terrestrial Ecology Program [NNX12AK56G] FX This study was funded by the National Science Foundation EaSM program (AGS-1049200) with a grant award to North Carolina State University, and the Eastern Forest Environment Threat Assessment Center (EFETAC), USDA Forest Service. J. Xiao was supported by NASA's Carbon Cycle Science Program (Grant # NNX14AJ18G) and Terrestrial Ecology Program (Grant # NNX12AK56G). NR 108 TC 7 Z9 7 U1 11 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 1 PY 2015 VL 353 BP 269 EP 279 DI 10.1016/j.foreco.2015.04.002 PG 11 WC Forestry SC Forestry GA CS4PQ UT WOS:000362058700027 ER PT J AU Qian, XQ Li, XC Ilori, TO Klein, JD Hughey, RP Li, CJ Alli, AA Guo, ZY Yu, P Song, X Chen, GP AF Qian, Xiaoqian Li, Xuechen Ilori, Titilayo O. Klein, Janet D. Hughey, Rebecca P. Li, Cong-jun Alli, Abdel A. Guo, Zhengyu Yu, Peng Song, Xiang Chen, Guangping TI RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla SO FRONTIERS IN PHYSIOLOGY LA English DT Article DE gene expression; Illumina; diabetes; urinary concentration; sialylation; fucosylation ID UREA TRANSPORTER UT-A1; COLLECTING DUCT; N-GLYCAN; GLYCOPROTEIN; VASOPRESSIN; MICE; SIALIDASE; PROTEINS; LATTICES; CLONING AB The UT-A1 urea transporter is crucial to the kidney's ability to generate concentrated urine. Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa. In diabetes, UT-Al protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria. However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown. In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-Al is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions. These changes were accompanied by altered UT-A1 association with the galectin proteins, beta-galactoside glycan binding proteins. To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT Al glycosylation using streptozotocin (STZ)-induced diabetic rat kidney. Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions. These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9. In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes. Conclusions: In diabetes, not only is UT-A1 protein abundance increased but the protein's glycan structure is also significantly changed. UT-A1 protein becomes highly sialylated, fucosylated and branched. Consistently, a number of crucial glycosylation related genes are changed under diabetic conditions. The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes. C1 [Qian, Xiaoqian; Li, Xuechen; Klein, Janet D.; Alli, Abdel A.; Chen, Guangping] Emory Univ, Sch Med, Dept Physiol, Atlanta, GA 30322 USA. [Qian, Xiaoqian; Song, Xiang] Harbin Med Univ, Affiliated Hosp 4, Dept Cardiol, Harbin 150001, Heilongiliang, Peoples R China. [Ilori, Titilayo O.; Klein, Janet D.; Chen, Guangping] Emory Univ, Sch Med, Dept Med, Div Renal, Atlanta, GA USA. [Hughey, Rebecca P.] Univ Pittsburgh, Sch Med, Dept Med, Renal Electrolyte Div, Pittsburgh, PA 15213 USA. [Hughey, Rebecca P.] Univ Pittsburgh, Sch Med, Dept Med, Dept Cell Biol & Physiol, Pittsburgh, PA 15213 USA. [Li, Cong-jun] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD USA. [Guo, Zhengyu; Yu, Peng] Texas A&M Univ, Dept Elect & Comp Engn, TEES AarLife Ctr Bioinformat & Genom Syst Engn, College Stn, TX USA. RP Song, X (reprint author), Harbin Med Univ, Affiliated Hosp 4, Dept Cardiol, 31 Yinhana St, Harbin 150001, Heilongiliang, Peoples R China. EM song761231@sina.com; gchen3@emory.edu FU NIH [R01-DK087838, DK054787]; Chinese National Natural Science Foundation [81300248]; Heilongjiang Science and Technology [LBH-Q13113] FX This work was supported by NIH grants R01-DK087838 (to GC), DK054787 (to RPH) and by Chinese National Natural Science Foundation Project 81300248 (to XS), Heilongjiang Science and Technology funding LBH-Q13113 (to XS). NR 42 TC 4 Z9 4 U1 2 U2 8 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-042X J9 FRONT PHYSIOL JI Front. Physiol. PD OCT 1 PY 2015 VL 6 AR 274 DI 10.3389/fphys.2015.00274 PG 11 WC Physiology SC Physiology GA CS9TA UT WOS:000362433500001 PM 26483702 ER PT J AU Ford, KL Baumgartner, K Henricot, B Bailey, AM Foster, GD AF Ford, Kathryn L. Baumgartner, Kendra Henricot, Beatrice Bailey, Andy M. Foster, Gary D. TI A reliable in vitro fruiting system for Armillaria mellea for evaluation of Agrobacterium tumefaciens transformation vectors SO FUNGAL BIOLOGY LA English DT Article DE Basidiocarp; Basidiomycete; Fungal transformation; Honey fungus; Mushroom ID AGARICUS-BISPORUS; MEDIATED TRANSFORMATION; COPRINOPSIS-CINEREA; BODY FORMATION; EXPRESSION; AFRICA; DNA; TEMPERATURE; PROMOTERS; TABESCENS AB Armillaria mellea is a serious pathogen of horticultural and agricultural systems in Europe and North America. The lack of a reliable in vitro fruiting system for heterothallic A. mellea has hindered research and required dependence on intermittently available wild-collected basidiospores of endemic genotypes, necessitating the use of variable genetic material in transformation studies. Here we describe a reliable, reproducible in vitro fruiting method for heterothallic A. mellea from the western US. Isolates and growth conditions were evaluated to determine effective fruiting conditions. Following medium colonisation for 4 weeks, cultures were incubated under warm/bright conditions for 4-6 weeks before incubation in dim/cool conditions. Primordia emerged within 3-4 weeks following a temperature decrease and this was most efficient when coupled with a light reduction. Basidiocarps matured within 3-4 weeks and produced viable basidiospores. Agrobacterium tumefaciens and vectors were evaluated by transformation of in vitro-produced basidiospores and a versatile transformation vector was constructed to simplify promoter and marker gene exchange using homologous recombination in yeast. Fruiting bodies and viable basidiospores of A. mellea have been reliably produced in vitro which, coupled with the enhanced knowledge of suitable A. tumefaciens strains and vectors for transformation, will assist future genetic research into this important pathogen. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Ford, Kathryn L.; Bailey, Andy M.; Foster, Gary D.] Univ Bristol, Sch Biol Sci, Bristol BS8 1TQ, Avon, England. [Baumgartner, Kendra] Univ Calif Davis, ARS, USDA, Davis, CA 95616 USA. [Henricot, Beatrice] Royal Hort Soc, Woking GU23 6QB, Surrey, England. RP Foster, GD (reprint author), Univ Bristol, Sch Biol Sci, Life Sci Bldg,24 Tyndall Ave, Bristol BS8 1TQ, Avon, England. EM Kathryn.Ford@bristol.ac.uk; K.Baumgartner@ucdavis.edu; Beatrice.henricot@forest-ry.gsi.gov.uk; Andy.Bailey@bristol.ac.uk; Gary.Foster@bristol.ac.uk OI Bailey, Andy/0000-0002-7594-3703 FU Royal Horticultural Society; United States Department of Agriculture under the DEFRA Plant Health Licence PHL [51046/198767/2] FX This research was funded in part by the Royal Horticultural Society and United States Department of Agriculture and was conducted under the DEFRA Plant Health Licence PHL 51046/198767/2. NR 44 TC 3 Z9 3 U1 3 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD OCT PY 2015 VL 119 IS 10 BP 859 EP 869 DI 10.1016/j.funbio.2015.06.007 PG 11 WC Mycology SC Mycology GA CS5RQ UT WOS:000362136100001 PM 26399182 ER PT J AU Sanjuan, TI Franco-Molano, AE Kepler, RM Spatafora, JW Tabima, J Vasco-Palacios, AM Restrepo, S AF Sanjuan, Tatiana I. Franco-Molano, Ana E. Kepler, Ryan M. Spatafora, Joseph W. Tabima, Javier Vasco-Palacios, Aida M. Restrepo, Silvia TI Five new species of entomopathogenic fungi from the Amazon and evolution of neotropical Ophiocordyceps SO FUNGAL BIOLOGY LA English DT Article DE Hypocreales; Formicidae; Fulgoridae; Host specificity; Diversity; Taxonomy ID TROPICAL FOREST ECOSYSTEMS; PHYLOGENETIC EVIDENCE; CORDYCEPS; GENUS; ANTS; FORMICIDAE; BEAUVERIA; DIVERSIFICATION; SUBFAMILIES; MORPHOLOGY AB The neotropical biogeographic zone is a 'hot spot' of global biodiversity, especially for insects. Fungal pathogens of insects appear to track this diversity. However, the integration of this unique component of fungal diversity into molecular phylogenetic analyses remains sparse. The entomopathogenic fungal genus Ophiocordyceps is species rich in this region with the first descriptions dating to the early nineteenth century. In this study, material from various ecosystems throughout Colombia and Ecuador was examined. Molecular phylogenetic analyses of five nuclear loci including SSU, LSU, TEF, RPB1, and RPB2 were conducted alongside a morphological evaluation. Thirty-five specimens were examined representing fifteen different species of Ophiocordyceps, and five new species, Ophiocordyceps blattarioides, Ophiocordyceps tiputini, Ophiocordyceps araracuarensis, Ophiocordyceps fulgoromorphila, and Ophiocordyceps evansii, were described. An accurate identification of the host allowed us to conclude that host identity and host habitat are positively correlated with phylogenetic species of Ophiocordyceps and are probably strong drivers for speciation of neotropical entomopathogenic fungi. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Sanjuan, Tatiana I.; Franco-Molano, Ana E.; Vasco-Palacios, Aida M.] Univ Antioquia, Lab Taxon & Ecol Hongos, Medellin 1226, Colombia. [Kepler, Ryan M.] USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Spatafora, Joseph W.; Tabima, Javier] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Vasco-Palacios, Aida M.] Fdn Biodiversa Colombia, Bogota 111311, Colombia. [Sanjuan, Tatiana I.; Restrepo, Silvia] Univ Los Andes, Lab Micol & Fitopatol, Bogota 111711, Colombia. RP Sanjuan, TI (reprint author), Univ Antioquia, Lab Taxon & Ecol Hongos, Calle 67 53-108, Medellin 1226, Colombia. EM t_sanjuan@hotmail.com OI Restrepo, Silvia/0000-0001-9016-1040; Tabima, Javier/0000-0002-3603-2691 FU Netherlands Fellowship Programmes (NFP) of the Netherlands organization for international cooperation in higher education (NUFFIC); National Science Foundation [DEB-0529752]; COLCIENCIAS (Department of Science and Technology of Colombia) [4892009] FX We thank Rodrigo Gallego, Oscar Cadena, Dimitri Forero for the identification of the hosts; Thomas Laessoe for making collections available for this study; Kent Brothers for his help in the English writing for the paper. This research was partially financed by the Netherlands Fellowship Programmes (NFP) of the Netherlands organization for international cooperation in higher education (NUFFIC) grant to Aida Vasco-P., the National Science Foundation in the form of a PEET grant to Joseph W. Spatafora (DEB-0529752). This publication is a partial result from the project, "The phylogenetic relationship of Cordyceps in the Insecta class on the Amazon of Colombia", executed by the authors and with additional funds from COLCIENCIAS (Department of Science and Technology of Colombia) 4892009. NR 63 TC 0 Z9 0 U1 8 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD OCT PY 2015 VL 119 IS 10 BP 901 EP 916 DI 10.1016/j.funbio.2015.06.010 PG 16 WC Mycology SC Mycology GA CS5RQ UT WOS:000362136100004 PM 26399185 ER PT J AU Troemel, ER Becnel, JJ AF Troemel, Emily R. Becnel, James J. TI Genome analysis and polar tube firing dynamics of mosquito-infecting microsporidia SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Polar tube; Microsporidia; Edhazardia aedis; Vavraia culicis; Mosquito; Genome sequence; Aedes aegypti ID GERMINATION; SEQUENCE; SPORES AB Microsporidia are highly divergent fungi that are obligate intracellular pathogens of a wide range of host organisms. Here we review recent findings from the genome sequences of mosquito-infecting microsporidian species Edhazardia aedis and Vavraia culicis, which show large differences in genome size, although similar numbers of predicted genes. We also show a video of E. aedis polar tube firing, which is the dramatic mechanism used by microsporidia to deliver the germ cell (sporoplasm) into the host cell to initiate intracellular infection. (C) 2015 Elsevier Inc. All rights reserved. C1 [Troemel, Emily R.] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA. [Becnel, James J.] USDA ARS, CMAVE, Gainesville, FL 32608 USA. RP Troemel, ER (reprint author), Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, 9500 Gilman Dr, La Jolla, CA 92093 USA. EM etroemel@ucsd.edu; James.Becnel@ars.usda.gov FU NIAID [R01 AI087528] FX E.R.T. acknowledges support from NIAID R01 AI087528. J.J.B. acknowledges support from the USDA-Agricultural Research Service and Neil Sanscrainte for video editing. NR 16 TC 1 Z9 1 U1 2 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD OCT PY 2015 VL 83 BP 41 EP 44 DI 10.1016/j.fgb.2015.08.007 PG 4 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA CS8BV UT WOS:000362311600005 PM 26300319 ER PT J AU Diep, CS Hingle, M Chen, TA Dadabhoy, HR Beltran, A Baranowski, J Subar, AF Baranowski, T AF Diep, Cassandra S. Hingle, Melanie Chen, Tzu-An Dadabhoy, Hafza R. Beltran, Alicia Baranowski, Janice Subar, Amy F. Baranowski, Tom TI The Automated Self-Administered 24-Hour Dietary Recall for Children, 2012 Version, for Youth Aged 9 to 11 Years: A Validation Study SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE 24-hour recall; Children; Computer; Diet assessment; Web-based ID BODY-MASS INDEX; RECORDING SOFTWARE SYSTEM; MULTIPLE-PASS METHOD; RETENTION INTERVAL; SCHOOL-LUNCH; REPORTING ACCURACY; FOOD-INTAKE; 4TH-GRADE CHILDREN; 13-YEAR-OLD CHILDREN; PHYSICAL-ACTIVITY AB Background Valid methods of diet assessment are important for nutrition research and practice, but can be difficult with children. Objective To validate the 2012 version of the Automated Self-Administered 24-Hour Dietary Recall for Children (ASA24-Kids-2012), a self-administered web-based 24-hour dietary recall (24hDR) instrument, among children aged 9 to 11 years, in two sites. Design Quasiexperimental. Participants/setting In one site, trained staff members observed and recorded foods and drinks consumed by children (n=38) during school lunch. The next day, the observed children completed both ASA24-Kids-2012 and an interviewer-administered 24hDR in a randomized order. Procedures in a second site (n=31) were similar, except observations occurred during dinner in a community location. Statistical analyses Foods were classified as matches (reported and consumed), intrusions (reported, but not consumed), or omissions (not reported, but consumed) for each participant. Rates of matches, intrusions, and omissions were calculated. Rates were compared between each recall method using repeated measures analysis of covariance. For matched foods, the authors determined correlation coefficients between observed and reported serving sizes. Results Match, intrusion, and omission rates between ASA24-Kids-2012 and observed intakes in Site 1 were 37%, 27%, and 35%, respectively. Comparable rates for interviewer-administered 24hDRs were 57%, 20%, and 23%, respectively. In Site 2, match, intrusion, and omission rates between ASA24-Kids-2012 and observed intakes were 53%, 12%, and 36%, respectively, vs 76% matches, 9% intrusions, and 15% omissions for interviewer-administered 24hDRs. The relationship strength between reported and observed serving sizes for matched foods was 0.18 in Site 1 and 0.09 in Site 2 for ASA24-Kids-2012, and 0.46 in Site 1 and 0.11 in Site 2 for interviewer-administered 24hDRs. Conclusions ASA24-Kids-2012 was less accurate than interviewer-administered 24hDRs when compared with observed intakes, but both performed poorly. Additional research should assess the age at which children can complete recalls without the help of a parent or guardian, as well as elucidate under which circumstances recalls can reasonably be used among children. C1 [Diep, Cassandra S.; Chen, Tzu-An; Dadabhoy, Hafza R.; Beltran, Alicia; Baranowski, Janice; Baranowski, Tom] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Hingle, Melanie] Univ Arizona, Coll Agr & Life Sci, Dept Nutr Sci, Tucson, AZ USA. [Subar, Amy F.] Natl Canc Inst, Div Canc Control & Populat Sci, Bethesda, MD USA. RP Diep, CS (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, 1100 Bates St, Houston, TX 77030 USA. EM cdiep@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institute of Diabetes and Digestive and Kidney Diseases [R01 DK091254]; US Department of Agriculture/Agricultural Research Service [58-6250-0-008]; Primary Care Research Training Grant from National Research Service Award [T32 HP10031] FX This research was funded by a grant from the National Institute of Diabetes and Digestive and Kidney Diseases (no. R01 DK091254) and by institutional support from the US Department of Agriculture/Agricultural Research Service under Cooperative Agreement no. 58-6250-0-008. C. S. Diep was supported fully by a Primary Care Research Training Grant from National Research Service Award (no. T32 HP10031). NR 58 TC 8 Z9 8 U1 0 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD OCT PY 2015 VL 115 IS 10 BP 1591 EP 1598 DI 10.1016/j.jand.2015.02.021 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CS5YL UT WOS:000362154100004 PM 25887784 ER PT J AU Liu, HH Liu, HQ Zhou, LN Zhang, ZH Zhang, X Wang, ML Li, HX Lin, ZW AF Liu, Huanhuan Liu, Hangqin Zhou, Leina Zhang, Zhihai Zhang, Xuan Wang, Mingli Li, Haixia Lin, Zhongwei TI Parallel Domestication of the Heading Date 1 Gene in Cereals SO MOLECULAR BIOLOGY AND EVOLUTION LA English DT Article DE comparative mapping; flowering time; parallel domestication ID RICE ORYZA-SATIVA; CULTIVATED RICE; FLOWERING TIME; FOXTAIL MILLET; POSTDOMESTICATION SPREAD; PHOTOPERIOD SENSITIVITY; POPULATION-GENETICS; SETARIA-ITALICA; MAIZE; GENOME AB Flowering time is one of the key determinants of crop adaptation to local environments during domestication. However, the genetic basis underlying flowering time is yet to be elucidated in most cereals. Although staple cereals, such as rice, maize, wheat, barley, and sorghum, have spread and adapted to a wide range of ecological environments during domestication, it is yet to be determined whether they have a common genetic basis for flowering time. In this study, we show, through map-based cloning, that flowering time in sorghum is controlled by a major quantitative trait locus (QTL) Heading Date 1 (HD1), located on chromosome 10. The causal gene encodes the CONSTANS gene family which contains a CCT domain. A 5-bp deletion of a minor allele present in the coding sequence leads to a gene frameshift that delays flowering in sorghum. In contrast, in foxtail millet, association mapping of HD1 showed a common causal site with a splicing variant from "GT" to "AT" that was highly correlated with flowering time. In addition, the rice HD1 gene is known to harbor several causal variants controlling flowering time. These data indicate that the major flowering time QTL HD1 was under parallel domestication in sorghum, foxtail millet, and rice. The pattern of common mixed minor, or even rare, causal alleles in HD1 across different species may be representative of the genetic basis of the domestication syndrome. Furthermore, large DNA sequence analysis of HD1 revealed multiple origins for domesticated sorghum and a single origin for domesticated foxtail millet. C1 [Liu, Huanhuan; Liu, Hangqin; Zhou, Leina; Zhang, Zhihai; Zhang, Xuan; Li, Haixia; Lin, Zhongwei] China Agr Univ, Dept Plant Genet & Breeding, Natl Maize Improvement Ctr, Beijing 100094, Peoples R China. [Wang, Mingli] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA USA. RP Lin, ZW (reprint author), China Agr Univ, Dept Plant Genet & Breeding, Natl Maize Improvement Ctr, Beijing 100094, Peoples R China. EM zlin@cau.edu.cn FU Chinese Thousand Talents Program [21983004]; National Science Foundation of China [91335108]; Ministry of Science and Technology [2012AA10A305] FX We thank Germplasm Resources Information Network and Chinese Crop Germplasm Resources Information System for providing all sorghum and foxtail millet accessions. This work was supported by the Chinese Thousand Talents Program (21983004 to Z. L.), National Science Foundation of China (91335108 to Z. L.), and the Ministry of Science and Technology (2012AA10A305 to Z. L.). NR 40 TC 3 Z9 4 U1 5 U2 30 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0737-4038 EI 1537-1719 J9 MOL BIOL EVOL JI Mol. Biol. Evol. PD OCT PY 2015 VL 32 IS 10 BP 2726 EP 2737 DI 10.1093/molbev/msv148 PG 12 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA CS3PQ UT WOS:000361987100020 PM 26116860 ER PT J AU Devi, MJ Sinclair, TR Taliercio, E AF Devi, Mura Jyostna Sinclair, Thomas R. Taliercio, Earl TI Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean SO PLOS ONE LA English DT Article ID WRKY TRANSCRIPTION FACTORS; DROUGHT STRESS; ARABIDOPSIS-THALIANA; WATER-DEFICIT; TRANSPIRATION RESPONSE; ABIOTIC STRESSES; STOMATAL DENSITY; FERULIC ACID; WHEAT LEAVES; PLANTS AB Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments. C1 [Devi, Mura Jyostna; Sinclair, Thomas R.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Taliercio, Earl] USDA ARS, Soybean & Nitrogen Fixat Unit, Raleigh, NC 27695 USA. RP Taliercio, E (reprint author), USDA ARS, Soybean & Nitrogen Fixat Unit, Raleigh, NC 27695 USA. EM Earl.Taliercio@ars.usda.gov FU United Soybean Board [1213] FX This work was funded by the United Soybean Board, Project 1213. NR 62 TC 2 Z9 2 U1 1 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 1 PY 2015 VL 10 IS 10 AR e0139134 DI 10.1371/journal.pone.0139134 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS6GT UT WOS:000362177100032 PM 26427064 ER PT J AU Kristensen, T Naesset, E Ohlson, M Bolstad, PV Kolka, R AF Kristensen, Terje Naesset, Erik Ohlson, Mikael Bolstad, Paul V. Kolka, Randall TI Mapping Above- and Below-Ground Carbon Pools in Boreal Forests: The Case for Airborne Lidar SO PLOS ONE LA English DT Article ID LASER SCANNER DATA; COARSE WOODY DEBRIS; FINE-ROOT BIOMASS; NORWAY SPRUCE; UNDERSTORY VEGETATION; OLD-GROWTH; SCOTS PINE; BIOPHYSICAL PROPERTIES; VACCINIUM-MYRTILLUS; INDIVIDUAL TREES AB A large and growing body of evidence has demonstrated that airborne scanning light detection and ranging (lidar) systems can be an effective tool in measuring and monitoring above-ground forest tree biomass. However, the potential of lidar as an all-round tool for assisting in assessment of carbon (C) stocks in soil and non-tree vegetation components of the forest ecosystem has been given much less attention. Here we combine the use airborne small footprint scanning lidar with fine-scale spatial C data relating to vegetation and the soil surface to describe and contrast the size and spatial distribution of C pools within and among multilayered Norway spruce (Picea abies) stands. Predictor variables from lidar derived metrics delivered precise models of above-and below-ground tree C, which comprised the largest C pool in our study stands. We also found evidence that lidar canopy data correlated well with the variation in field layer C stock, consisting mainly of ericaceous dwarf shrubs and herbaceous plants. However, lidar metrics derived directly from understory echoes did not yield significant models. Furthermore, our results indicate that the variation in both the mosses and soil organic layer C stock plots appears less influenced by differences in stand structure properties than topographical gradients. By using topographical models from lidar ground returns we were able to establish a strong correlation between lidar data and the organic layer C stock at a stand level. Increasing the topographical resolution from plot averages (similar to 2000 m(2)) towards individual grid cells (1 m(2)) did not yield consistent models. Our study demonstrates a connection between the size and distribution of different forest C pools and models derived from airborne lidar data, providing a foundation for future research concerning the use of lidar for assessing and monitoring boreal forest C. C1 [Kristensen, Terje; Naesset, Erik; Ohlson, Mikael] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, As, Norway. [Kristensen, Terje; Bolstad, Paul V.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Kolka, Randall] US Forest Serv, USDA, Grand Rapids, MN USA. RP Kristensen, T (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM terjekrist@gmail.com OI Kristensen, Terje/0000-0002-4542-2915 FU Fulbright Foundation FX Funding was provided by Fulbright Foundation, http://www.cies.org/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 102 TC 1 Z9 1 U1 10 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 1 PY 2015 VL 10 IS 10 AR e0138450 DI 10.1371/journal.pone.0138450 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS6GT UT WOS:000362177100014 PM 26426532 ER PT J AU Stulberg, MJ Huang, Q AF Stulberg, Michael J. Huang, Qi TI A TaqMan-Based Multiplex qPCR Assay and DNA Extraction Method for Phylotype IIB Sequevars 1&2 (Select Agent) Strains of Ralstonia solanacearum SO PLOS ONE LA English DT Article ID POLYMERASE-CHAIN-REACTION; BLOOD-DISEASE BACTERIUM; REAL-TIME PCR; 3 BIOVAR 2; PSEUDOMONAS-SOLANACEARUM; SEQUENCE-ANALYSIS; SP-NOV; AMPLIFICATION; GERANIUM; RACE-3 AB Ralstonia solanacearum race 3 biovar 2 strains belonging to phylotype IIB, sequevars 1 and 2 (IIB-1&2) cause brown rot of potato in temperate climates, and are quarantined pathogens in Canada and Europe. Since these strains are not established in the U.S. and because of their potential risk to the potato industry, the U.S. government has listed them as select agents. Cultivated geraniums are also a host and have the potential to spread the pathogen through trade, and its extracts strongly inhibits DNA-based detection methods. We designed four primer and probe sets for an improved qPCR method that targets stable regions of DNA. RsSA1 and RsSA2 recognize IIB-1&2 strains, RsII recognizes the current phylotype II (the newly proposed R. solanacearum species) strains (and a non-plant associated R. mannitolilytica), and Cox1 recognizes eight plant species including major hosts of R. solanacearum such as potato, tomato and cultivated geranium as an internal plant control. We multiplexed the RsSA2 with the RsII and Cox1 sets to provide two layers of detection of a positive IIB-1&2 sample, and to validate plant extracts and qPCR reactions. The TaqMan-based uniplex and multiplex qPCR assays correctly identified 34 IIB-1&2 and 52 phylotype II strains out of 90 R. solanacearum species complex strains. Additionally, the multiplex qPCR assay was validated successfully using 169 artificially inoculated symptomatic and asymptomatic plant samples from multiple plant hosts including geranium. Furthermore, we developed an extraction buffer that allowed for a quick and easy DNA extraction from infected plants including geranium for detection of R. solanacearum by qPCR. Our multiplex qPCR assay, especially when coupled with the quick extraction buffer method, allows for quick, easy and reliable detection and differentiation of the IIB-1&2 strains of R. solanacearum. C1 [Stulberg, Michael J.; Huang, Qi] USDA, Floral & Nursery Plants Res Unit, ARS, Beltsville, MD 20705 USA. [Stulberg, Michael J.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. RP Huang, Q (reprint author), USDA, Floral & Nursery Plants Res Unit, ARS, Beltsville, MD 20705 USA. EM qi.huang@ars.usda.gov FU U.S. Department of Agriculture (USDA); Agricultuiral Reseach Service (ARS); Animal and Plant Health Inspection Service; U.S. Department of Energy (DOE); USDA; DOE [DE-AC05-06OR23100] FX This research was financially supported by the U.S. Department of Agriculture (USDA), Agricultuiral Reseach Service (ARS) and Animal and Plant Health Inspection Service. It was supported in part by an appointment to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. NR 35 TC 2 Z9 2 U1 0 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 1 PY 2015 VL 10 IS 10 AR e0139637 DI 10.1371/journal.pone.0139637 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS6GT UT WOS:000362177100084 PM 26426354 ER PT J AU Oliveira, TFB Bertechini, AG Bricka, RM Kim, EJ Gerard, PD Peebles, ED AF Oliveira, T. F. B. Bertechini, A. G. Bricka, R. M. Kim, E. J. Gerard, P. D. Peebles, E. D. TI Effects of in ovo injection of organic zinc, manganese, and copper on the hatchability and bone parameters of broiler hatchlings SO POULTRY SCIENCE LA English DT Article DE bone quality; embryonic; incubation; in ovo enrichment ID SOMATIC CHARACTERISTICS; NUTRIENT PROFILES; GROWTH-RATE; EMBRYOS; DEFICIENCY; STRENGTH; 25-HYDROXYCHOLECALCIFEROL; MINERALIZATION; PERFORMANCE; METABOLISM AB Effects of the in ovo injection of commercial diluent containing supplemental microminerals (Zn, Mn, and Cu) on hatchability and hatching chick quality variables in Ross x Ross 708 broilers were examined. On 17 d of incubation (doi) eggs were subjected to 1 of 4 treatments using a commercial multi-egg injector. Treatments included non-injected (treatment 1) and diluent-injected (treatment 2) control groups. Those in treatment 3 received diluent containing 0.181, 0.087, and 0.010 mg/mL of Zn, Mn, and Cu, respectively, and those in treatment 4 received diluent containing 0.544, 0.260, and 0.030 mg/mL of Zn, Mn, and Cu, respectively. A total of 1,872 eggs were distributed among 4 treatment groups on each of 6 replicate tray levels. Hatchability of fertile eggs set (HF) was determined on 20.5 and 21.5 doi. On 21.5 doi, HF and mean hatching chick weight (MHW) were determined. One bird from each treatment replicate group was randomly selected, weighed, and necropsied for the extraction of their livers and tibiae. The tibiae fresh and dry weight, length, width, bone breaking strength (BBS), and percentage of bone ash (PBA) were determined. The dry livers were weighed and ashed. Injection treatment had no significant effect on HF at 20.5 doi. However, there was a significant injection treatment effect on HF at 21.5 doi. The HF of eggs at 21.5 doi in treatment 4 was significantly lower than that of the non-injected control group, with treatment 3 being intermediate. Furthermore, there were no significant treatment effects noted for MHW fresh and dry tibia weights, tibia length and width, tibia length to weight ratio, BBS, liver ash content, or percentage of minerals (Ca, P, Mg, Mn, and Zn) in the tibia ash. However, embryos from eggs that received treatment 4 had a significantly higher PBA in comparison to all other treatments. In conclusion, although treatment 4 negatively affected HF, the injection of diluent containing the high micromineral concentration has the potential to improve bone mineralization. C1 [Oliveira, T. F. B.; Bertechini, A. G.] Univ Fed Lavras, Dept Anim Sci, BR-37200000 Lavras, Brazil. [Bricka, R. M.] Mississippi State Univ, Dept Chem Engn, Mississippi State, MS 39762 USA. [Kim, E. J.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. [Gerard, P. D.] Clemson Univ, Dept Math Sci, Clemson, SC 29634 USA. [Peebles, E. D.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. RP Peebles, ED (reprint author), Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. EM d.peebles@msstate.edu FU [MIS-322270] FX This is Journal Article Number J-12645 from the Mississippi Agricultural and Forestry Experiment Station supported by MIS-322270. Use of trade names in this publication does not imply endorsement by Mississippi Agricultural and Forestry Experiment Station of these products, nor similar ones not mentioned. NR 39 TC 2 Z9 2 U1 4 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD OCT PY 2015 VL 94 IS 10 BP 2488 EP 2494 DI 10.3382/ps/pev248 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CS3BH UT WOS:000361945600022 PM 26330613 ER PT J AU Niu, ZS Bai, FL Sun, T Tian, H Yu, D Yin, JC Li, SM Li, TH Cao, HW Yu, QZ Wu, YZ Ren, GP Li, DS AF Niu, Zeshan Bai, Fuliang Sun, Tian Tian, Hui Yu, Dan Yin, Jiechao Li, Siming Li, Tianhe Cao, Hongwei Yu, Qingzhong Wu, Yunzhou Ren, Guiping Li, Deshan TI Recombinant Newcastle Disease virus Expressing IL15 Demonstrates Promising Antitumor Efficiency in Melanoma Model SO TECHNOLOGY IN CANCER RESEARCH & TREATMENT LA English DT Article DE IL15; IL2; Melanoma; rNDV; Safer; Tumor agent AB Recombinant Newcastle Disease Virus (rNDV) has shown oncolytic therapeutic effect in preclinical studies. Previous data indicate that rNDV carrying IL2 has shown promise in cancer therapy. Due to the significant side effects of IL2, IL15 has been introduced into cancer therapy. A number of studies have suggested that IL15 efficiently enhances the activities of CTL and NK cells and inhibits the tumor recurrence and metastasis. Furthermore, IL15 is less toxic than IL2. Therefore, we hypothesize that a recombinant NDV expressing IL15 would be a promising agent for the treatment of malignant tumors. The human IL15 gene or IL2 gene was incorporated into the genome of lentogenic LaSota strain at the position between the HN and L genes (namely rNDV-IL15 or rNDV-IL2). The two viruses efficiently infected tumor cells and expressed IL15 or IL2 protein. Melanoma tumor-bearing mice were treated by intra-tumoral (i.t.) injection of rNDV-IL15 or rNDV-IL2. Both rNDV-IL15 and rNDV-IL2 effectively suppressed tumor growth compared with rNDV. The 120-day survival rate of rNDV-IL15- treated group was 12.5% higher than that of rNDV-IL2 group, although the difference was not statistically significant, both recombinant viruses had strong abilities to induce CD41 T cell and CTL cell responses. However, rNDV-IL15 significantly induced more IFN- release and stimulated more CD81 T cells infiltration in the tumor sites compared with rNDV-IL2. In the tumor re-challenged experiment, the survival rates of rNDV-IL15 group and rNDV-IL2 group were statistically higher than that of PBS group. The survival rate of rNDV-IL15 group was 26.67% higher than that of rNDV-IL2 group although the difference was not statistically significant. In conclusion, rNDV-IL15 is a promising antitumor agent against melanoma. C1 [Niu, Zeshan; Bai, Fuliang; Sun, Tian; Tian, Hui; Yu, Dan; Yin, Jiechao; Li, Siming; Wu, Yunzhou; Ren, Guiping; Li, Deshan] Northeast Agr Univ, Coll Life Sci, Harbin, Peoples R China. [Li, Tianhe] Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China. [Cao, Hongwei] Heilongjiang Bayi Agr Univ, Coll Biol Sci & Technol, Daqing, Peoples R China. [Yu, Qingzhong] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Li, DS (reprint author), Northeast Agr Univ, Coll Life Sci, Mucai St 59, Harbin, Peoples R China. EM deshanli@163.com FU National Natural Science Foundation of China (NSFC) [31200121]; Heilongjiang Funds for Young Scientists [QC2012C099]; Northeast Agriculture University Funds for Ph.D [2012RCB43] FX This study was supported by grants from the National Natural Science Foundation of China (NSFC, Grant No. 31200121), and the Heilongjiang Funds for Young Scientists (QC2012C099) and Northeast Agriculture University Funds for Ph.D. (2012RCB43). NR 0 TC 4 Z9 4 U1 1 U2 6 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1533-0346 EI 1533-0338 J9 TECHNOL CANCER RES T JI Technol. Cancer Res. Treat. PD OCT PY 2015 VL 14 IS 5 BP 607 EP 615 DI 10.7785/tcrt.2012.500414 PG 9 WC Oncology SC Oncology GA CR7KF UT WOS:000361527700012 PM 24645750 ER PT J AU Egan, JF Hafla, A Goslee, S AF Egan, J. Franklin Hafla, Aimee Goslee, Sarah TI Tradeoffs between production and perennial vegetation in dairy farming systems vary among counties in the northeastern US SO AGRICULTURAL SYSTEMS LA English DT Article DE Dairy farms; Grazing; Perennial forages; Phosphorus; Regional food systems ID UNITED-STATES; ENVIRONMENTAL-IMPACT; FORAGE YIELD; SUSTAINABILITY; AGRICULTURE; PHOSPHORUS; MANAGEMENT; LIVESTOCK; CROPLAND; PLANT AB Dairy farms that grow more perennial vegetation as grazing pastures or conserved forages can offer many environmental benefits but may show reduced milk production relative to farms feeding higher amounts of grain and corn silage. Because yields of annual and perennial crops vary with soil type, an accurate comparison of the productive potential of these systems over county or regional scales may require taking into account spatial variation in soil quality. In this study, we present a novel approach to calculate the production from dairy systems that adjusts average crop yields to the productive potential of local soils using the National Commodity Crop Productivity Index (NCCPI). We used on-farm survey data to define confinement and grazing systems with varying amounts of perennial forage and applied our method to a sample of five counties in the northeast United States. High corn silage farm systems produced 21 to 168% more milk per hectare of farmland than grazingbased farm systems, but variation among counties was greater than variation among systems, with the best (Lancaster, PA) producing as much as 5.3 times more than the least (Orange, VT). Adjusting yields for soil productivity had smaller effects on milk production than differences in farm system or county. On average, grazing farm systems generally produced slightly more milk when yields were adjusted using the NCCPI (8%) while high corn silage systems showed a moderate decrease (13%). Compared to scenarios of all local crop production, scenarios with unlimited corn and soybean imports often more than doubled county-scale milk production. Restricting grain imports to prevent excess phosphorus resulted in a 3-15% decrease in milk production relative to unlimited imports, but still produced far more milk than in the all local production scenarios. Sensitivity analysis of the model showed that milk production in each county was very responsive to changes in perennial forage yields (especially for grazing systems), responsive to changes in average daily milk production per cow, and generally not responsive to changes in the productive lifetime of lactating cows. This study demonstrates a persistent tradeoff between perenniality and production in dairy systems, but suggests that opportunities may exist to maintain current milk production levels in the Northeast while also expanding land cover in perennial vegetation. Published by Elsevier Ltd. C1 [Egan, J. Franklin; Hafla, Aimee; Goslee, Sarah] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Egan, JF (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, Bldg 3702,Curtin Rd, University Pk, PA 16802 USA. EM Franklin.Egan@ars.usda.gov NR 77 TC 2 Z9 2 U1 2 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-521X EI 1873-2267 J9 AGR SYST JI Agric. Syst. PD OCT PY 2015 VL 139 BP 17 EP 28 DI 10.1016/j.agsy.2015.06.004 PG 12 WC Agriculture, Multidisciplinary SC Agriculture GA CS0VE UT WOS:000361779500002 ER PT J AU Zhang, HY Mi, TJ Khan, OY Sheng, YJ Eremin, SA Beier, RC Zhang, SX Shen, JZ Wang, ZH AF Zhang, Huiyan Mi, Tiejun Khan, Oleg Yu Sheng, Yajie Eremin, Sergei A. Beier, Ross C. Zhang, Suxia Shen, Jianzhong Wang, Zhanhui TI Fluorescence polarization immunoassay using IgY antibodies for detection of valnemulin in swine tissue SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE Fluorescence polarization immunoassay; IgY; Chemical residues; Swine tissue; Valnemulin ID IN-VITRO AB Immunoglobulin Y (IgY) is derived from egg yolk and has been identified as a cheap and high-yield immunoreagent. The application of IgY in immunoassays for the detection of chemical contaminants in food samples has rarely been reported. In this work, we describe a rapid and sensitive fluorescence polarization immunoassay (FPIA) for valnemulin (VAL) using IgY which was produced using a previously prepared immunogen. Three fluorophore-labeled VAL tracers were synthesized and the sensitivity of the best tracer (VAL-DTAF) in the optimized FPIA with antibody IgY(100) demonstrated an IC50 value of 12 ng mL(-1) in buffer. After evaluation of several extraction procedures, acidified acetonitrile was selected to extract VAL from swine tissue. The recoveries of VAL in spiked swine tissue at three levels (50, 100, and 200 mu g kg(-1)) were higher than 79 % with coefficients of variation (CVs) lower than 12 %. The limit of detection (LOD) of the FPIA in swine tissue was 26 mu g kg(-1) and was lower than the maximum residue limit (MRL) of VAL set by the European Union. The study showed that IgY could be a good substitute for IgG when developing a high-throughput assay for chemical residues. C1 [Zhang, Huiyan; Mi, Tiejun; Sheng, Yajie; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui] China Agr Univ, Beijing Key Lab Detect Technol Anim Derived Food, Beijing Lab Food Qual & Safety, Coll Vet Med,State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China. [Khan, Oleg Yu; Eremin, Sergei A.] Moscow MV Lomonosov State Univ, Fac Chem, Dept Chem Enzymol, Moscow 119992, Russia. [Beier, Ross C.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Wang, ZH (reprint author), China Agr Univ, Beijing Key Lab Detect Technol Anim Derived Food, Beijing Lab Food Qual & Safety, Coll Vet Med,State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China. EM wangzhanhui@cau.edu.cn FU Trans-Century Training Program Foundation for the Talents from the Ministry of Education [NCET-12-0529]; Special Fund of Basic Scientific Research in Colleges [2013QJ048] FX This work was supported by grants from both the Trans-Century Training Program Foundation for the Talents from the Ministry of Education (NCET-12-0529) and the Special Fund of 2013 Basic Scientific Research in Colleges (2013QJ048) NR 18 TC 2 Z9 2 U1 5 U2 32 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 EI 1618-2650 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD OCT PY 2015 VL 407 IS 25 BP 7843 EP 7848 DI 10.1007/s00216-015-8948-z PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CR8WA UT WOS:000361633700030 PM 26277187 ER PT J AU Jordan, DB Braker, JD AF Jordan, Douglas B. Braker, Jay D. TI Rate-limiting steps of a stereochemistry retaining beta-D-xylosidase from Geobacillus stearothermophilus acting on four substrates SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article DE Glycoside hydrolase; beta-xylosidase; Burst-steady state; Dexylosylation; Rate limitation; Kinetic data reinterpretation ID FAMILY-52 GLYCOSIDE HYDROLASE; ENZYME; T-6 AB Kinetic experiments of GSXynB2, a GH52 retaining beta-xylosidase, acting on 2-nitrophenyl-beta-D-xylopyranoside (2NPX), 4-nitrophenyl-beta-D-xylopyranoside (4NPX), 4-methylumbelliferyl-beta-D-xylopyranoside (MuX) and xylobiose (X2) were conducted at pH 7.0 and 25 degrees C. Catalysis proceeds in two steps (xylodidation followed by dexylosidation): E + substrate TO E-xylose + leaving group TO E + xylose. kcat falls into two groups: 4NPX (1.95 s(-1)) and 2NPX, MuX and X2 (15.8 s(-1), 12.6 s(-1), 12.8 s(-1), respectively). Dexylosylation (E-xylose to E + xylose), the common step for the enzymatic hydrolysis of the four substrates, must exceed 15.8 s(-1). kcat of 4NPX would seem mainly limited by xylosylation (step 1) and the other three substrates would seem mainly limited by dexylosylation (step 2) - a conclusion that critically lacks chemical justification (compare 4NPX and 2NPX). Presteady-state rates indicate rapid xylosidation rates for all substrates so a later step (not dexylosidation) is rate-limiting for 4NPX. That 2NPX is an onlier and 4NPX is an outlier (both leaving group pK(a) of 7.2) of the Bronsted plot pattern (logk(cat) vs pK(a) of phenol leaving group) is thus possibly explained by 4NP release. The pH dependency of k(cat) 2NPX encompasses 2 bell-shaped curves with peaks of pH 3 and pH 7. Published by Elsevier Inc. C1 [Jordan, Douglas B.; Braker, Jay D.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Jordan, DB (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM douglas.jordan@ars.usda.gov NR 10 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0003-9861 EI 1096-0384 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD OCT 1 PY 2015 VL 583 BP 73 EP 78 DI 10.1016/j.abb.2015.08.004 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA CR9AA UT WOS:000361644400009 PM 26271441 ER PT J AU Valles, SM Porter, SD AF Valles, Steven M. Porter, Sanford D. TI Dose response of red imported fire ant colonies to Solenopsis invicta virus 3 SO ARCHIVES OF VIROLOGY LA English DT Article ID STRAND RNA VIRUS; SUCCESSFUL TRANSMISSION; HYMENOPTERA-FORMICIDAE; HOST-SPECIFICITY; POPULATIONS; DISCOVERY; DISEASE AB Baiting tests were conducted to evaluate the effect of increasing Solenopsis invicta virus 3 (SINV-3) dose on fire ant colonies. Actively growing early-stage fire ant (Solenopsis invicta Buren) laboratory colonies were pulse-exposed for 24 hours to six concentrations of SINV-3 (10(1), 10(3), 10(5), 10(7), 10(9) genome equivalents/mu l) in 1 ml of a 10 % sucrose bait and monitored regularly for two months. SINV-3 concentration had a significant effect on colony health. Brood rating (proportion of brood to worker ants) began to depart from the control group at 19 days for the 10(9) concentration and 26 days for the 10(7) concentration. At 60 days, brood rating was significantly lower among colonies treated with 10(9), 10(7), and 10(5) SINV-3 concentrations. The intermediate concentration, 10(5), appeared to cause a chronic, low-level infection with one colony (n = 9) supporting virus replication. Newly synthesized virus was not detected in any fire ant colonies treated at the 10(1) concentration, indicating that active infections failed to be established at this level of exposure. The highest bait concentration chosen, 10(9), appeared most effective from a control aspect; mean colony brood rating at this concentration (1.1 +/- 0.9 at the 60 day time point) indicated poor colony health with minimal brood production. No clear relationship was observed between the quantity of plus genome strand detected and brood rating. Conversely, there was a strong relationship between the presence of the replicative genome strand and declining brood rating, which may serve as a predictor of disease severity. Recommendations for field treatment levels to control fire ants with SINV-3 are discussed. C1 [Valles, Steven M.; Porter, Sanford D.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Valles, SM (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov NR 30 TC 3 Z9 3 U1 2 U2 15 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD OCT PY 2015 VL 160 IS 10 BP 2407 EP 2413 DI 10.1007/s00705-015-2520-1 PG 7 WC Virology SC Virology GA CS1IN UT WOS:000361817600002 PM 26162304 ER PT J AU Nemchinov, L AF Nemchinov, Lev TI Comment on "The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus)" SO ARCHIVES OF VIROLOGY LA English DT Letter C1 USDA ARS, Inst Plant Sci, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Nemchinov, L (reprint author), USDA ARS, Inst Plant Sci, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM Lev.Nemchinov@ars.usda.gov NR 1 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD OCT PY 2015 VL 160 IS 10 BP 2655 EP 2655 DI 10.1007/s00705-015-2594-9 PG 1 WC Virology SC Virology GA CS1IN UT WOS:000361817600034 PM 26381670 ER PT J AU Weber, GM Hostuttler, MA Semmens, KJ Beers, BA AF Weber, Gregory M. Hostuttler, Mark A. Semmens, Kenneth J. Beers, Brian A. TI Induction and viability of tetraploids in brook trout (Salvelinus fontinalis) SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID ONCORHYNCHUS-MYKISS EMBRYOS; PERCH PERCA-FLAVESCENS; RAINBOW-TROUT; HEAT-SHOCK; 1ST CLEAVAGE; DIPLOID FEMALES; MUD LOACH; PERFORMANCE; PLOIDY; TRIPLOIDY AB Brook trout (Salvelinus fontinalis) populations are threatened by introduction of invasive species, habitat loss, and habitat degradation in their native range and are a problem invasive species in western Unites States and Canada and in Europe. Stocking sterile triploids has been promoted as an approach to reduce negative effects of stocking of brook trout for recreational fishing on native fish populations. Crossing a tetraploid with a diploid is a method of triploid production that may help hatcheries meet demand. We induced tetraploidy in brook trout by application of 633 kg.cm(-2) of hydrostatic pressure for 8 min at 70%-72.5% of the first cleavage interval. Yields of above 50% tetraploid progeny at hatching were readily achieved, although few animals reached 1 year of age. We crossed a male tetraploid with female diploid fish and produced interploid-triploids with eyeing rates in excess of 50%, demonstrating male tetraploids are fertile and capable of siring triploid progeny. Female tetraploid fish were reared to 16 months posthatching and possessed follicles in secondary vitellogenesis, suggesting tetraploid females are also fertile. Tetraploid induction rates in excess of 96% were achieved applying the same hydrostatic pressure treatment to zygotes of tetraploid x diploid crosses at 30 min postfertilization. C1 [Weber, Gregory M.; Hostuttler, Mark A.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Semmens, Kenneth J.] Kentucky State Univ, Aquaculture Res Ctr, Frankfort, KY 40601 USA. [Beers, Brian A.] Virginia Dept Game & Inland Fisheries, Paint Bank Fish Culture Stn, Paint Bank, VA 24131 USA. RP Weber, GM (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM greg.weber@ars.usda.gov FU Agricultural Research Service Project [1930-31000-010-000D] FX We thank Josh Kretzer, Jill Birkett, and Meghan Manor for laboratory assistance, including FCI determination and tetraploidy induction; Vicky Blazer and Kathy Springs at USGS for the histology; animal caretaking contributions from Josh Kretzer, Jenea McGowan, and Kyle Jenkins at NCCCWA; and the staff at PBFCS. Funding for this study came from the Agricultural Research Service Project 1930-31000-010-000D. Mention of trade names is solely for the purpose of providing accurate information and should not imply product endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 40 TC 0 Z9 0 U1 1 U2 23 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD OCT PY 2015 VL 72 IS 10 BP 1443 EP 1449 DI 10.1139/cjfas-2014-0536 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CS1LZ UT WOS:000361828400001 ER PT J AU Wiechmann, ML Hurteau, MD North, MP Koch, GW Jerabkova, L AF Wiechmann, Morgan L. Hurteau, Matthew D. North, Malcolm P. Koch, George W. Jerabkova, Lucie TI The carbon balance of reducing wildfire risk and restoring process: an analysis of 10-year post-treatment carbon dynamics in a mixed-conifer forest SO CLIMATIC CHANGE LA English DT Article ID UNDERSTORY PLANT-COMMUNITIES; FUEL-REDUCTION TREATMENTS; NORTHERN SIERRA-NEVADA; WESTERN UNITED-STATES; PONDEROSA PINE FOREST; CLIMATE-CHANGE; PRESCRIBED-FIRE; TREE MORTALITY; THINNING TREATMENT; MANAGING FORESTS AB Forests sequester carbon from the atmosphere, helping mitigate climate change. In fire-prone forests, burn events result in direct and indirect emissions of carbon. High fire-induced tree mortality can cause a transition from a carbon sink to source, but thinning and prescribed burning can reduce fire severity and carbon loss when wildfire occurs. However, treatment implementation requires carbon removal and emissions to reduce high-severity fire risk. The carbon removed and emitted during treatment may be resequestered by subsequent tree growth, although there is much uncertainty regarding the length of time required. To assess the long-term carbon dynamics of thinning and burning treatments, we quantified the 10-year post-treatment carbon stocks and 10-year net biome productivity (NBP) from a full-factorial experiment involving three levels of thinning and two levels of burning in a mixed-conifer forest in California's Sierra Nevada. Our results indicate that (1) the understory thin treatment, that retained large trees, quickly recovered the initial carbon emissions (NBP = 31.4 +/- 4.2 Mg C ha(-1)), (2) the carbon emitted from prescribed fire in the burn-only treatment was resequestered within the historical fire return interval (NBP = 32.8 +/- 3.5 Mg C ha(-1)), and (3) the most effective treatment for reducing fire risk, understory thin and burn, had negative NBP (-6.0 +/- 4.5 Mg C ha(-1)) because of post-fire large tree mortality. Understory thinning and prescribed burning can help stabilize forest carbon and restore ecosystem resilience, but this requires additional emissions beyond only thinning or only burning. Retaining additional mid-sized trees may reduce the carbon impacts of understory thinning and burning. C1 [Wiechmann, Morgan L.] Penn State Univ, IGDP Ecol, University Pk, PA 16802 USA. [Wiechmann, Morgan L.; Hurteau, Matthew D.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [North, Malcolm P.] USDA, Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Koch, George W.] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Koch, George W.] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA. [Jerabkova, Lucie] Univ Calif Davis, Dept Plant Sci, Davis, CA 95618 USA. [Jerabkova, Lucie] Czech Univ Life Sci Prague, Dept Forest Management, Prague, Czech Republic. RP Wiechmann, ML (reprint author), Penn State Univ, IGDP Ecol, University Pk, PA 16802 USA. EM mlw331@psu.edu RI Hurteau, Matthew/D-2301-2009 OI Hurteau, Matthew/0000-0001-8457-8974 FU Joint Fire Science Program [10-1-10-14] FX We acknowledge funding from the Joint Fire Science Program (10-1-10-14). We thank J.P. Kaye and A.H. Taylor for constructive feedback. NR 68 TC 2 Z9 2 U1 3 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD OCT PY 2015 VL 132 IS 4 BP 709 EP 719 DI 10.1007/s10584-015-1450-y PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CS1CC UT WOS:000361799100018 ER PT J AU Wiechmann, ML Hurteau, MD North, MP Koch, GW Jerabkova, L AF Wiechmann, Morgan L. Hurteau, Matthew D. North, Malcolm P. Koch, George W. Jerabkova, Lucie TI The carbon balance of reducing wildfire risk and restoring process: an analysis of 10-year post-treatment carbon dynamics in a mixed-conifer forest (vol 132, pg 709, 2015) SO CLIMATIC CHANGE LA English DT Correction C1 [Wiechmann, Morgan L.] Penn State Univ, IGDP Ecol, University Pk, PA 16802 USA. [Wiechmann, Morgan L.; Hurteau, Matthew D.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [North, Malcolm P.] USDA, Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Koch, George W.] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Koch, George W.] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA. [Jerabkova, Lucie] Univ Calif Davis, Dept Plant Sci, Davis, CA 95618 USA. [Jerabkova, Lucie] Czech Univ Life Sci Prague, Dept Forest Management, Prague, Czech Republic. RP Wiechmann, ML (reprint author), Penn State Univ, IGDP Ecol, University Pk, PA 16802 USA. EM mlw331@psu.edu RI Hurteau, Matthew/D-2301-2009 OI Hurteau, Matthew/0000-0001-8457-8974 NR 1 TC 0 Z9 0 U1 1 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD OCT PY 2015 VL 132 IS 4 BP 721 EP 722 DI 10.1007/s10584-015-1484-1 PG 2 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CS1CC UT WOS:000361799100019 ER PT J AU Stergiopoulos, S Brown, C Felix, T Grampp, G Getz, K AF Stergiopoulos, S. Brown, C. Felix, T. Grampp, G. Getz, K. TI Health Care Professional Perspectives on Adverse Event Reporting by Treatment Setting in the United States: Underreporting and Educational Gap Insights SO DRUG SAFETY LA English DT Meeting Abstract CT 15th Annual Meeting of the International-Society-of-Pharmacovigilance (ISoP) - Cubism in Pharmacovigilance CY OCT 27-30, 2015 CL Prague, CZECH REPUBLIC SP Int Soc Pharmacovigilance ID DRUG EVENTS C1 [Stergiopoulos, S.; Getz, K.] Tufts Univ, Tufts Ctr Study Drug Dev, Boston, MA 02111 USA. [Brown, C.] Tufts Univ, HNRC, Boston, MA 02111 USA. [Felix, T.; Grampp, G.] Amgen Inc, Global Regulatory Affairs & Safety, Thousand Oaks, CA 91320 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU ADIS INT LTD PI NORTHCOTE PA 5 THE WAREHOUSE WAY, NORTHCOTE 0627, AUCKLAND, NEW ZEALAND SN 0114-5916 EI 1179-1942 J9 DRUG SAFETY JI Drug Saf. PD OCT PY 2015 VL 38 IS 10 MA P 156 BP 1030 EP 1030 PG 1 WC Public, Environmental & Occupational Health; Pharmacology & Pharmacy; Toxicology SC Public, Environmental & Occupational Health; Pharmacology & Pharmacy; Toxicology GA CS0PK UT WOS:000361764000219 ER PT J AU Sankaran, S Khot, LR Espinoza, CZ Jarolmasjed, S Sathuvalli, VR Vandemark, GJ Miklas, PN Carter, AH Pumphrey, MO Knowles, NR Payek, MJ AF Sankaran, Sindhuja Khot, Lav R. Espinoza, Carlos Zuniga Jarolmasjed, Sanaz Sathuvalli, Vidyasagar R. Vandemark, George J. Miklas, Phillip N. Carter, Arron H. Pumphrey, Michael O. Knowles, N. Richard Payek, Mark J. TI Low-altitude, high-resolution aerial imaging systems for row and field crop phenotyping: A review SO EUROPEAN JOURNAL OF AGRONOMY LA English DT Review DE Aerial imaging; Data mining; Crop breeding; High-throughput field phenomics ID WATER-STRESS INDEX; CHLOROPHYLL FLUORESCENCE; CANOPY TEMPERATURE; NITROGEN STATUS; WINTER-WHEAT; PLANT STRESS; MULTISPECTRAL FLUORESCENCE; PRECISION AGRICULTURE; UNMANNED HELICOPTER; SUNFLOWER LEAVES AB Global plant genetics research efforts have focused on developing high yielding, stress tolerant, and disease resistant row and field crop varieties that are more efficient in their use of agronomfc inputs (water, nutrients, pesticides, etc.). Until recently, a key bottleneck in such research was the lack of high-throughput sensing technologies for effective and rapid evaluation of expressed phenotypes under field conditions for holistic data-driven decision making and variety selection. This review focuses on technological aspects of integrating unmanned aerial vehicles with imaging systems to enhance field phenotyping capabilities. The state-of-the-art of unmanned aerial vehicle technology for various applications including crop emergence, vigor, and characterization of yield potential of row and field crops has been reviewed. The potential of using aerial imaging to evaluate resistance/susceptibility to biotic and abiotic stress for crop breeding and precision production management has been discussed along with future perspectives and developments. (C) 2015 Elsevier B.V. All rights reserved. C1 [Sankaran, Sindhuja; Khot, Lav R.; Espinoza, Carlos Zuniga; Jarolmasjed, Sanaz] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. [Sankaran, Sindhuja; Khot, Lav R.] Washington State Univ, Dept Biol Syst Engn, Ctr Precis & Automated Agr Syst, Prosser, WA 99350 USA. [Sathuvalli, Vidyasagar R.] Oregon State Univ, Dept Crop & Soil Sci, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. [Vandemark, George J.] ARS, Grain Legume Genet & Physiol Res Unit, USDA, Pullman, WA 99164 USA. [Miklas, Phillip N.] ARS, Vegetable & Forage Crops Prod Res Unit, USDA, Prosser, WA 99350 USA. [Carter, Arron H.; Pumphrey, Michael O.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Knowles, N. Richard; Payek, Mark J.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. RP Sankaran, S (reprint author), Washington State Univ, Dept Biol Syst Engn, POB 646120, Pullman, WA 99164 USA. EM sindhuja.sankaran@wsu.edu FU USDA National Institute for Food and Agriculture, Hatch Project [1002864 (WNP00821)] FX This activity was funded, in part, by USDA National Institute for Food and Agriculture, Hatch Project, 1002864 (WNP00821). NR 143 TC 20 Z9 20 U1 33 U2 115 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1161-0301 EI 1873-7331 J9 EUR J AGRON JI Eur. J. Agron. PD OCT PY 2015 VL 70 BP 112 EP 123 DI 10.1016/j.eja.2015.07.004 PG 12 WC Agronomy SC Agriculture GA CR8CX UT WOS:000361579800012 ER PT J AU Hu, CS Zhao, Y Li, KC Zhu, JY Gleisner, R AF Hu, Chuanshuang Zhao, Yu Li, Kecheng Zhu, J. Y. Gleisner, Roland TI Optimizing cellulose fibrillation for the production of cellulose nanofibrils by a disk grinder SO HOLZFORSCHUNG LA English DT Article DE cellulose nanofibrils (CNF); disk grinding; fibrillation efficiency; field emission-scanning electron microscopy (FE-SEM); microfibrillated cellulose (MFC); nanocellulose ID PULP; FIBER AB The fibrillation of a bleached kraft eucalyptus pulp was investigated by means of a laboratory-scale disk grinder for the production of cellulose nanofibrils (CNF), while the parameters disk rotating speed, solid loading, and fibrillation duration were varied. The cumulative energy consumption was monitored during fibrillation. The degree of polymerization (DP) and water retention value (WRV) of the resultant cellulose fibrils were determined as measures of the degree of fibrillation, which was also visualized by scanning electron microscopy, field emission-scanning electron microscopy, and transmission electron microscopy imaging. A higher rotating speed than 1500 rpm did not improve the fibrillation judged by DP and WRV measurements. Solid loading has an insignificant effect on fibrillation in a wide range. The energy consumption (E) was determined as a function of the DP and WRV. The optimal grinding conditions were between 1200 and 1500 rpm at 2.0%-2.2% solid loading. C1 [Hu, Chuanshuang; Zhu, J. Y.; Gleisner, Roland] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. [Hu, Chuanshuang] South China Agr Univ, Coll Forestry, Guangzhou, Guangdong, Peoples R China. [Zhao, Yu; Li, Kecheng] Univ New Brunswick, Dept Chem Engn, Fredericton, NB E3B 5A3, Canada. RP Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, USDA, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM jzhu@fs.fed.us FU Department of Education of Guangdong Province [YQ2013029]; USDA Agriculture and Food Research Initiative [2011-67009-20056]; Chinese Scholarship Council FX We acknowledge the financial supports from the Department of Education of Guangdong Province (YQ2013029), the USDA Agriculture and Food Research Initiative through a competitive grant (No. 2011-67009-20056), and the Chinese Scholarship Council. The funding from these programs made the visiting appointment of Chuanshuang Hu at the USDA Forest Products Laboratory possible. NR 26 TC 5 Z9 5 U1 12 U2 27 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD OCT PY 2015 VL 69 IS 8 BP 993 EP 1000 DI 10.1515/hf-2014-0219 PG 8 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA CS1MW UT WOS:000361831200009 ER PT J AU Lu, KY White, RH Fu, F Hou, JF Zhang, YS Gribbins, N Cai, ZY AF Lu, Keyang White, Robert H. Fu, Feng Hou, Junfeng Zhang, Yisheng Gribbins, Neil Cai, Zhiyong TI Reinforced hybrid wood-aluminum composites with excellent fire performance SO HOLZFORSCHUNG LA English DT Article DE aluminum alloy sheet; fire performance; mechanical property; wood-aluminum composite (WAC) ID LAMINATED VENEER LUMBER; CONNECTIONS; RETARDANTS; PLATES AB The fire performance of several types of wood-aluminum composites (WAC) was analyzed by the small vertical furnace test. The time needed to reach the temperature of 139 degrees C/181 degrees C (T-139 degrees C/181 degrees C) and the linear char rate of 300 degrees C (L-300 degrees C) were obtained by evaluating the fire performance of WAC. The T-139 degrees C/181 degrees C values ranged from 23.6 to 44.8 min. The presence and position of the aluminum alloy sheet remarkably affected the fire performance of WAC. In addition to an initial delay of 19 min, the L-300 degrees C also increased when the aluminum alloy sheet was located on the surface. However, the times for 300 degrees C only increased slightly when the aluminum alloy sheet was in the middle. The initial delay observed for the aluminum alloy sheet on the surface was reduced by more than 50% when the wood veneer was located on the surface of the aluminum alloy sheet. The mechanical properties of WAC were also investigated. It was concluded that the uniformity and strength of different composites was improved after the lamination of the aluminum alloy sheet. In addition, the modulus of elasticity of WAC quadrupled, and the bonding strength between the aluminum alloy sheet and the oriented strand board (OSB) was greater than that of the OSB. C1 [Lu, Keyang; Fu, Feng; Hou, Junfeng; Zhang, Yisheng] Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China. [Lu, Keyang; White, Robert H.; Gribbins, Neil; Cai, Zhiyong] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Hou, Junfeng] Zhejiang Agr & Forestry Univ, Coll Engn, Linan 311300, Peoples R China. RP Lu, KY (reprint author), Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China. EM luky@caf.ac.cn; zcai@fs.fed.us FU Chinese Academy of Forestry (CAF) [201104004] FX The authors thank the industry special fund project (201104004) and outstanding scholar teaching of Chinese Academy of Forestry (CAF) for supporting paper. We also greatly appreciated associate Professor Youming Yu (Zhejiang Agriculture RI Forestry University) for his technical assistance during the analyzing of bonding interface. NR 28 TC 0 Z9 0 U1 2 U2 7 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD OCT PY 2015 VL 69 IS 8 BP 1027 EP 1037 DI 10.1515/hf-2014-0099 PG 11 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA CS1MW UT WOS:000361831200013 ER PT J AU Foth, AJ Brown-Brandl, T Hanford, KJ Miller, PS Gomez, GG Kononoff, PJ AF Foth, A. J. Brown-Brandl, T. Hanford, K. J. Miller, P. S. Gomez, G. Garcia Kononoff, P. J. TI Energy content of reduced-fat dried distillers grains with solubles for lactating dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy cow; energy balance; indirect calorimetry; reduced-fat dried distillers grains with solubles ID SILAGE-BASED DIETS; ENTERIC METHANE; BEEF-CATTLE; RUMEN FERMENTATION; MILK-PRODUCTION; HOLSTEIN COWS; GRASS-SILAGE; CORN-SILAGE; EMISSIONS; NITROGEN AB Eight Holstein and 8 Jersey multiparous, lactating cows were used to complete 56 energy balances to determine the energy content of reduced-fat dried distillers grains with solubles (RFDDGS). A repeated switchback design was used to compare treatments with and without RFDDGS. Diets consisted of 24.2% corn silage, 18.4% alfalfa hay, 6.94% brome hay with either 22.9% rolled corn or 14.8% soybean meal (control), or 8.95% rolled corn, 28.8% RFDDGS, and 0% soybean meal [Co-P; dry-matter (DM) basis]. The inclusion of RFDDGS did not affect DM intake, averaging 21.4 0.53 kg of DM for all cows, but milk production tended to increase from 29.8 to 30.9 +/- 1.46 kg/d for control and Co-P treatments, respectively. Milk fat percentage and energy-corrected milk did not differ between treatments, averaging 4.33 +/- 0.14% and 34.1 kg/d, respectively. Milk protein was significantly decreased by the Co-P treatment (3.56 and 3.41 +/- 0.08% for control and Co-P treatments), but protein yield was not affected. Milk energies were 1.40 Mcal/d greater with Co-P. Energy lost as methane was reduced by 0.31 Mcal/d with the addition of RFDDGS to the diet. Heat loss averaged 29.9 +/- 0.55 Mcal/d and was not different between diets. Average energy retained as tissue energy was -2.99 +/- 0.93 Mcal/d and did not differ between treatments. Intake of digestible and metabolizable energy were not different between the control and Co-P treatments, averaging 2.68 and 2.31 Mcal/kg of DM, respectively. The net energy of lactation values of control and Co-P diets were calculated to be 1.43 and 1.47 Mcal/kg of DM, respectively. These energy estimates suggest greater energy content of diets containing RFDDGS than diets containing a mixture of corn and soybean meal in lactating dairy cows. C1 [Foth, A. J.; Miller, P. S.; Gomez, G. Garcia; Kononoff, P. J.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Brown-Brandl, T.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Hanford, K. J.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. RP Kononoff, PJ (reprint author), Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. EM pkononoff2@unl.edu FU Poet LLC (Sioux Falls, SD) FX The authors thank the employees of the University of Nebraska-Lincoln Dairy Research Facility for care and management of all experimental animals. Additional gratitude is expressed to Poet LLC (Sioux Falls, SD) for partial financial support and K. Karges, formerly of Poet LLC, for his feedback and ideas. NR 42 TC 1 Z9 1 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD OCT PY 2015 VL 98 IS 10 BP 7142 EP 7152 DI 10.3168/jds.2014-9226 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CS1YS UT WOS:000361865400048 PM 26233444 ER PT J AU Yuan, Q Jordan, R Brlansky, RH Istomina, O Hartung, J AF Yuan, Qing Jordan, Ramon Brlansky, Ronald H. Istomina, Olga Hartung, John TI Development of single chain variable fragment (scFv) antibodies against Xylella fastidiosa subsp pauca by phage display SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Xylella fastidiosa; Single chain variable fragment; Phage display ID CITRUS VARIEGATED CHLOROSIS; 16S-23S INTERGENIC SPACER; 16S RDNA; GENETIC DIVERSITY; AXENIC CULTURE; CLONAL DIVERSITY; PIERCES-DISEASE; COSTA-RICA; SAO-PAULO; STRAINS AB Xylella fastidiosa is a member of the gamma proteobacteria. It is fastidious, insect-vectored and xylem-limited and causes a variety of diseases, some severe, on a wide range of economically important perennial crops, including grape and citrus. Antibody based detection assays are commercially available for X. fastidiosa, and are effective at the species, but not at the subspecies level. We have made a library of scFv antibody fragments directed against X. fastidiosa subsp. pauca strain 9a5c (citrus) by using phage display technology. Antibody gene repertoires were PCR-amplified using 23 primers for the heavy chain variable region (V-H) and 21 primers for the light chain variable region (V-L). The V-H and V-L, were joined by overlap extension PCR, and then the genes of the scFv library were ligated into the phage vector pKM19. The library contained 1.2 x 10(7) independent clones with full-length scFv inserts. In each of 3 cycles of affinity-selection with 9a5c, about 1.0 x 10(12) phage were used for panning with 4.1 x 10(6), 7.1 x 10(6), 2.1 x 10(7) phage recovered after the first, second and third cycles, respectively. Sixty-six percent of clones from the final library bound X fastidiosa 9a5c in an ELISA. Some of these scFv antibodies recognized strain 9a5c and did not recognize X. fastidiosa strains that cause Pierce's disease of grapevine. Published by Elsevier B.V. C1 [Yuan, Qing; Hartung, John] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Jordan, Ramon] USDA ARS, Floral & Nursery Plants Res Unit, US Natl Arboretum, Beltsville, MD 20705 USA. [Brlansky, Ronald H.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Istomina, Olga] Sigma Tau Pharmaceut, Rome, Italy. [Yuan, Qing] Sichuan Med Univ, Luzhou 646000, Sichuan Provinc, Peoples R China. RP Hartung, J (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM john.hartung@ars.usda.gov FU Citrus Research and Development Foundation, Lake Alfred, FL [95]; USDA ARS [8042-22000-281-00D] FX This research was funded in part by reimbursable agreement #95 with the Citrus Research and Development Foundation, Lake Alfred, FL and by project 8042-22000-281-00D of the USDA ARS. NR 37 TC 1 Z9 1 U1 1 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD OCT PY 2015 VL 117 BP 148 EP 154 DI 10.1016/j.mimet.2015.07.020 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA CS1XA UT WOS:000361861000026 PM 26232710 ER PT J AU Zielinski, W AF Zielinski, William J. TI When prey provide more than food: mammalian predators appropriating the refugia of their prey SO MAMMAL RESEARCH LA English DT Article DE Predator-prey; Dens; Herbivore; Behavior; Habitat; Resting; Foraging ID PINE MARTENS; SOUTH-DAKOTA; PALLASS CATS; HABITAT USE; SWIFT FOX; KIT FOX; DIET; MONGOOSES; SELECTION; ECOLOGY AB Some mammalian predators acquire both food and shelter from their prey, by eating them and using the refugia the prey construct. I searched the literature for examples of predators that exhibit this behavior and summarize their taxonomic affiliations, relative sizes, and distributions. I hypothesized that size ratios of species involved in this dynamic would be near 1.0, and that most of these interactions would occur at intermediate and high latitudes. Seventeen species of Carnivorans exploited at least 23 species of herbivores as food and for their refugia. Most of them (76.4 %) were in the Mustelidae; several small species of canids and a few herpestids were exceptions. Surprisingly, the average predator/prey weight ratio was 10.51, but few species of predators were more than ten times the weight of the prey whose refugia they exploit. This may be why the long and thin Mustelines commonly exploit this habit. A number of predators appropriate the refugia of their key prey during winter when their prey occupies thermally secure nests. Indeed, most of the predator-prey pairs that engage in this relationship occur in intermediate and high latitudes, though there may be a reporting bias. Predators that depend on prey as food and for shelter, and whose fates are linked strongly to a few key prey species, may be particularly vulnerable to changes in climate that affect the subnivean habitats of their prey. Mammals that create refugia that can be used by other species (among them predators) may play disproportionately important roles in their communities. C1 USDA Forest Serv, Redwood Sci Lab, Pacific Southwest Res Stn, Arcata, CA 95521 USA. RP Zielinski, W (reprint author), USDA Forest Serv, Redwood Sci Lab, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU USDA Forest Service, Pacific Southwest Research Station FX I thank S. LaPoint, J. Lewis, and R. Green for information and conversations that contributed to the paper and R. Schlexer and M. Zielinski for editorial assistance. This work was funded by the USDA Forest Service, Pacific Southwest Research Station. NR 61 TC 0 Z9 0 U1 6 U2 23 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 2199-2401 EI 2199-241X J9 MAMMAL RES JI Mammal Res. PD OCT PY 2015 VL 60 IS 4 BP 285 EP 292 DI 10.1007/s13364-015-0236-y PG 8 WC Zoology SC Zoology GA CR9UI UT WOS:000361702400002 ER PT J AU Guo, LY Huang, YF Chen, X Hu-Li, J Urban, JF Paul, WE AF Guo, Liying Huang, Yuefeng Chen, Xi Hu-Li, Jane Urban, Joseph F., Jr. Paul, William E. TI Innate immunological function of T(H)2 cells in vivo SO NATURE IMMUNOLOGY LA English DT Article ID CHRONIC HELMINTH INFECTIONS; LYMPHOID-CELLS; AIRWAY INFLAMMATION; CYTOKINE PRODUCTION; HYGIENE HYPOTHESIS; IMMUNE-RESPONSES; IL-1 FAMILY; DISEASE; ASTHMA; INTERLEUKIN-13 AB Type 2 helper T cells (T(H)2 cells) produce interleukin 13 (IL-13) when stimulated by papain or house dust mite extract (HDM) and induce eosinophilic inflammation. This innate response is dependent on IL-33 but not T cell antigen receptors (TCRs). While type 2 innate lymphoid cells (ILC2 cells) are the dominant innate producers of IL-13 in naive mice, we found here that helminth-infected mice had more T(H)2 cells compared to uninfected mice, and these cells became major mediators of innate type 2 responses. T(H)2 cells made important contributions to HDM-induced antigen-nonspecific eosinophilic inflammation and protected mice recovering from infection with Ascaris suum against subsequent infection with the phylogenetically distant nematode Nippostrongylus brasiliensis. Our findings reveal a previously unappreciated role for effector T(H)2 cells during TCR-independent innate-like immune responses. C1 [Guo, Liying; Huang, Yuefeng; Chen, Xi; Hu-Li, Jane; Paul, William E.] NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. RP Guo, LY (reprint author), NIAID, Immunol Lab, NIH, Bldg 10, Bethesda, MD 20892 USA. EM lguo@niaid.nih.gov; wpaul@niaid.nih.gov FU Division of Intramural Research of the National Institute of Allergy and Infectious Diseases (US National Institute of Health) FX We thank A.N. McKenzie (Medical Research Council Laboratory of Molecular Biology, Cambridge, UK) for Il1rl1-/- mice; J.-P. Girard (Centre National de la Recherche Scientifique, Institute de Pharmacology et de Biologie Structurale) for Il33-LacZ gene-trap reporter mice; J. Ward for help with pathology analysis; and J. Edwards and K. Weng for help with cell sorting. Supported by the Division of Intramural Research of the National Institute of Allergy and Infectious Diseases (US National Institute of Health). NR 38 TC 27 Z9 27 U1 3 U2 16 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1529-2908 EI 1529-2916 J9 NAT IMMUNOL JI Nat. Immunol. PD OCT PY 2015 VL 16 IS 10 BP 1051 EP + DI 10.1038/ni.3244 PG 11 WC Immunology SC Immunology GA CR9OO UT WOS:000361686500010 PM 26322482 ER PT J AU Panickar, KS Qin, BL Anderson, RA AF Panickar, Kiran S. Qin, Bolin Anderson, Richard A. TI Ischemia-induced endothelial cell swelling and mitochondrial dysfunction are attenuated by cinnamtannin D1, green tea extract, and resveratrol in vitro SO NUTRITIONAL NEUROSCIENCE LA English DT Article DE Stroke; Edema; BBB; Oxidative stress; Cinnamon; Green tea ID MONOCYTE CHEMOATTRACTANT PROTEIN-1; OXYGEN-GLUCOSE DEPRIVATION; CEREBRAL-ISCHEMIA; BRAIN EDEMA; GLUTAMATE UPTAKE; CULTURED ASTROCYTES; OXIDATIVE STRESS; NEURONAL DAMAGE; GLIAL-CELLS; POLYPHENOLS AB Polyphenols possess antioxidant and anti-inflammatory properties. Oxidative stress (OS) and inflammation have been implicated in the pathogenesis of cytotoxic brain edema in cerebral ischemia. In addition, OS and pro-inflammatory cytokines also damage the endothelial cells and the neurovascular unit. Endothelial cell swelling may contribute to a leaky blood-brain barrier which may result in vasogenic edema in the continued presence of the existing cytotoxic edema. We investigated the protective effects of polyphenols on cytotoxic cell swelling in bEND3 endothelial cultures subjected to 5 hours oxygen-glucose deprivation (OGD). A polyphenol trimer from cinnamon (cinnamtannin D1), a polyphenol-rich extract from green tea, and resveratrol prevented the OGD-induced rise in mitochondrial free radicals, cell swelling, and the dissipation of the inner mitochondrial membrane potential. Monocyte chemoattractant protein (also called CCL2), a chemokine, but not tumor necrosis factor-a or interleukin-6, augmented the cell swelling. This effect of monochemoattractant protein 1-1 was attenuated by the polyphenols. Cyclosporin A, a blocker of the mitochondrial permeability transition pore, did not attenuate cell swelling but BAPTA-AM, an intracellular calcium chelator did, indicating a role of [Ca2+](i) but not the mPT in cell swelling. These results indicate that the polyphenols reduce mitochondrial reactive oxygen species and subsequent cell swelling in endothelial cells following ischemic injury and thus may reduce brain edema and associated neural damage in ischemia. One possible mechanism by which the polyphenols may attenuate endothelial cell swelling is through the reduction in [Ca2+](i). C1 [Panickar, Kiran S.; Qin, Bolin] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. [Panickar, Kiran S.; Anderson, Richard A.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Qin, Bolin] Integr Nutraceut Int, Springhill, TN 37174 USA. RP Panickar, KS (reprint author), Hillspet Nutr Ctr, Topeka, KS 66603 USA. EM kiran_panickar@hillspet.com FU US Department of Agriculture FX US Department of Agriculture. NR 55 TC 2 Z9 2 U1 3 U2 8 PU MANEY PUBLISHING PI LEEDS PA STE 1C, JOSEPHS WELL, HANOVER WALK, LEEDS LS3 1AB, W YORKS, ENGLAND SN 1028-415X EI 1476-8305 J9 NUTR NEUROSCI JI Nutr. Neurosci. PD OCT PY 2015 VL 18 IS 7 BP 297 EP 306 DI 10.1179/1476830514Y.0000000127 PG 10 WC Neurosciences; Nutrition & Dietetics SC Neurosciences & Neurology; Nutrition & Dietetics GA CS1ST UT WOS:000361848700002 PM 24773045 ER PT J AU Hidalgo, B Aslibekyan, S Wiener, HW Irvin, MR Straka, RJ Borecki, IB Tiwari, HK Tsai, MY Hopkins, PN Ordovas, JM Arnett, DK AF Hidalgo, Bertha Aslibekyan, Stella Wiener, Howard W. Irvin, Marguerite R. Straka, Robert J. Borecki, Ingrid B. Tiwari, Hemant K. Tsai, Michael Y. Hopkins, Paul N. Ordovas, Jose M. Arnett, Donna K. TI A family-specific linkage analysis of blood lipid response to fenofibrate in the Genetics of Lipid Lowering Drug and Diet Network SO PHARMACOGENETICS AND GENOMICS LA English DT Article DE family study; fenofibrate; lipids ID SEGREGATION AB Cost-effective identification of novel pharmacogenetic variants remains a pressing need in the field. Using data from the Genetics of Lipid Lowering Drugs and Diet Network, we identified genomic regions of relevance to fenofibrate response in a sample of 173 families. Our approach included a multipoint linkage scan, followed by selection of the families showing evidence of linkage. We identified a strong signal for changes in LDL-cholesterol (LDL-C) on chromosome 7 (peak logarithm of odds score=4.76) in the full sample (n=821). The signal for LDL-C response remained even after adjusting for baseline LDL-C. Restricting analyses only to the families contributing to the linkage signal for LDL-C (N=19), we observed a peak logarithm of odds score of 5.17 for chromosome 7. Two genes under this peak (ABCB4 and CD36) were of biological interest. These results suggest that linked family analyses might be a useful approach to gene discovery in the presence of a complex (e.g. multigenic) phenotype. C1 [Hidalgo, Bertha; Aslibekyan, Stella; Wiener, Howard W.; Irvin, Marguerite R.; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Sch Publ Hlth, Birmingham, AL 35294 USA. [Tiwari, Hemant K.] Univ Alabama Birmingham, Dept Biostat, Sch Publ Hlth, Birmingham, AL 35294 USA. [Straka, Robert J.] Univ Minnesota, Coll Pharm, Dept Expt & Clin Pharmacol, Minneapolis, MN 55455 USA. [Tsai, Michael Y.] Univ Minnesota, Sch Publ Hlth, Dept Med & Pathol, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Medford, MA 02155 USA. [Borecki, Ingrid B.] Univ Washington, Sch Med, Genome Sci Ctr, Div Stat Genom, Seattle, WA USA. [Hopkins, Paul N.] Univ Utah, Sch Med, Dept Internal Med, Div Cardiovasc Genet, Salt Lake City, UT USA. [Ordovas, Jose M.] CNIC, Dept Epidemiol Atherothrombosis & Imaging, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados IMDEA Alimentac, Madrid, Spain. RP Hidalgo, B (reprint author), Univ Alabama Birmingham, Dept Epidemiol, RPHB 220D, Birmingham, AL 35294 USA. EM bhidalgo@uab.edu FU National Heart, Lung, and Blood Institute [U01HL072524-04] FX This study was funded by National Heart, Lung, and Blood Institute grant U01HL072524-04. NR 13 TC 1 Z9 1 U1 1 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1744-6872 EI 1744-6880 J9 PHARMACOGENET GENOM JI Pharmacogenet. Genomics PD OCT PY 2015 VL 25 IS 10 BP 511 EP 514 DI 10.1097/FPC.0000000000000162 PG 4 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA CR6SB UT WOS:000361476500005 PM 26203732 ER PT J AU Ippolito, JA AF Ippolito, J. A. TI Aluminum-Based Water Treatment Residual Use in a Constructed Wetland for Capturing Urban Runoff Phosphorus: Column Study SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Aluminum-based water treatment residuals; Creeping red fescue; Engineered wetland; Phosphorus sorption; Phosphorus storage capacity; Urban runoff ID BY-PRODUCTS; SORPTION CAPACITY; BUFFER STRIPS; SLUDGE; RETENTION; BIOSOLIDS; GROWTH; SOIL; PHOSPHATE; REMOVAL AB Aluminum-based water treatment residuals (Al-WTRs) have a strong affinity to sorb P. In a proof-of-concept greenhouse column study, Al-WTR was surface-applied at rates equivalent to 0, 62, 124, and 248 Mg ha(-1) to 15 cm of soil on top of 46 cm of sand; Al-WTR rates were estimated to capture 0, 10, 20, and 40 years of P from an urban watershed entering an engineered wetland in Boise, ID, USA. Creeping red fescue (Festuca rubra) was established in all columns; one set of columns received no Al-WTR or plants. After plant establishment, once per week over a 12-week period, similar to 1.0 pore volumes of similar to 0.20 mg P L-1 were added to each column. Infiltration rates were measured, leachate was collected and analyzed for soluble P, and fescue yield, P concentration, and uptake were determined. After plant harvest, the sand, soil, and the Al-WTR layer were collected and analyzed for Olsen P; amorphous Al, Fe, and P; P storage capacity (PSC); and soluble + Al + Fe-bound, occluded, and Ca-bound P phases. Infiltration rate increased only due to the presence of plants. Leached P decreased (50 %) with plants present; Al-WTR further reduced soluble P leaching losses (60 %). Fescue yield, P concentration, and uptake increased with increasing Al-WTR rate, due to Al-WTR sorbing and potentially making P more plant available; Olsen-extractable P increased with increasing Al-WTR rate, supporting this contention. The PSC was reduced with the 62 Mg ha(-1) Al-WTR rate but maintained with greater Al-WTR rates. The 124 and 248 Mg ha(-1) Al-WTR rates also contained greater P associated with the soluble + Al + Fe and occluded phases which should be stable over the long term (e.g., decadal). It was recommended to apply Al-WTR near the 124 and 248 Mg ha(-1) rates in the future to capture urban runoff soluble P in the Boise, ID, engineered wetland. C1 USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. RP Ippolito, JA (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600E, Kimberly, ID 83341 USA. EM jim.ippolito@ars.usda.gov NR 36 TC 2 Z9 2 U1 4 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD OCT PY 2015 VL 226 IS 10 AR 334 DI 10.1007/s11270-015-2604-2 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA CS0PN UT WOS:000361764400013 ER PT J AU Turner, TL Zhang, GC Kim, S Subramaniam, V Steffen, D Skory, CD Jang, JY Yu, BJ Jin, YS AF Turner, Timothy L. Zhang, Guo-Chang Kim, Soo Rin Subramaniam, Vijay Steffen, David Skory, Christopher D. Jang, Ji Yeon Yu, Byung Jo Jin, Yong-Su TI Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Lactic acid; Xylose; Saccharomyces cerevisiae; Lignocellulose ID PERMEASE GENE JEN1; PYRUVATE DECARBOXYLASE; RHIZOPUS-ORYZAE; LACTATE-DEHYDROGENASE; EFFICIENT PRODUCTION; ETHANOL-PRODUCTION; LDH GENE; YEAST; FERMENTATION; EXPRESSION AB Production of lactic acid from renewable sugars has received growing attention as lactic acid can be used for making renewable and bio-based plastics. However, most prior studies have focused on production of lactic acid from glucose despite that cellulosic hydrolysates contain xylose as well as glucose. Microbial strains capable of fermenting both glucose and xylose into lactic acid are needed for sustainable and economic lactic acid production. In this study, we introduced a lactic acid-producing pathway into an engineered Saccharomyces cerevisiae capable of fermenting xylose. Specifically, ldhA from the fungi Rhizopus oryzae was overexpressed under the control of the PGK1 promoter through integration of the expression cassette in the chromosome. The resulting strain exhibited a high lactate dehydrogenase activity and produced lactic acid from glucose or xylose. Interestingly, we observed that the engineered strain exhibited substrate-dependent product formation. When the engineered yeast was cultured on glucose, the major fermentation product was ethanol while lactic acid was a minor product. In contrast, the engineered yeast produced lactic acid almost exclusively when cultured on xylose under oxygen-limited conditions. The yields of ethanol and lactic acid from glucose were 0.31 g ethanol/g glucose and 0.22 g lactic acid/g glucose, respectively. On xylose, the yields of ethanol and lactic acid were < 0.01 g ethanol/g xylose and 0.69 g lactic acid/g xylose, respectively. These results demonstrate that lactic acid can be produced from xylose with a high yield by S. cerevisiae without deleting pyruvate decarboxylase, and the formation patterns of fermentations can be altered by substrates. C1 [Turner, Timothy L.; Zhang, Guo-Chang; Jin, Yong-Su] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Turner, Timothy L.; Zhang, Guo-Chang; Subramaniam, Vijay; Steffen, David; Jin, Yong-Su] Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA. [Kim, Soo Rin] Kyungpook Natl Univ, Sch Food Sci & Biotechnol, Taegu 702701, South Korea. [Skory, Christopher D.] USDA ARS, Natl Ctr Agr Utilizat Res, Renewable Prod Technol RPT Res Unit, Peoria, IL 61604 USA. [Jang, Ji Yeon; Yu, Byung Jo] Korea Inst Ind Technol KITECH, Chungcheong Reg Div, IT Convergence Mat Grp, Cheonan, South Korea. RP Jin, YS (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, 260 Bevier Hall, Urbana, IL 61801 USA. EM ysjin@illinois.edu OI Kim, Soo Rin/0000-0001-5855-643X FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2016-67011-22806]; University of Illinois at Urbana-Champaign's JBT Graduate Fellowship FX This study was funded by the Agriculture and Food Research Initiative Competitive Grant No. 2016-67011-22806 from the USDA National Institute of Food and Agriculture. TLT received additional funding support from the University of Illinois at Urbana-Champaign's JBT Graduate Fellowship. NR 43 TC 5 Z9 5 U1 2 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD OCT PY 2015 VL 99 IS 19 BP 8023 EP 8033 DI 10.1007/s00253-015-6701-3 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CQ8ER UT WOS:000360839800019 PM 26043971 ER PT J AU Schmelcher, M Powell, AM Camp, MJ Pohl, CS Donovan, DM AF Schmelcher, Mathias Powell, Anne M. Camp, Mary J. Pohl, Calvin S. Donovan, David M. TI Synergistic streptococcal phage lambda SA2 and B30 endolysins kill streptococci in cow milk and in a mouse model of mastitis SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Peptidoglycan hydrolase; Endolysin; Bacteriophage; Antimicrobial; Mastitis; Streptococcus ID WALL BINDING DOMAINS; SOMATIC-CELL COUNT; STAPHYLOCOCCUS-AUREUS; BOVINE MASTITIS; LYTIC ENZYMES; MAMMARY-GLAND; BACTERIOPHAGE ENDOLYSINS; BACTERIOLYTIC AGENT; ESCHERICHIA-COLI; TRANSGENIC MICE AB Bovine mastitis results in billion dollar losses annually in the USA alone. Streptococci are among the most relevant causative agents of this disease. Conventional antibiotic therapy is often unsuccessful and contributes to development of antibiotic resistance. Bacteriophage endolysins represent a new class of antimicrobials against these bacteria. In this work, we characterized the endolysins (lysins) of the streptococcal phages lambda SA2 and B30 and evaluated their potential as anti-mastitis agents. When tested in vitro against live streptococci, both enzymes exhibited near-optimum lytic activities at ionic strengths, pH, and Ca2+ concentrations consistent with cow milk. When tested in combination in a checkerboard assay, the lysins were found to exhibit strong synergy. The lambda SA2 lysin displayed high activity in milk against Streptococcus dysgalactiae (reduction of CFU/ml by 3.5 log units at 100 mu g/ml), Streptococcus agalactiae (2 log), and Streptococcus uberis (4 log), whereas the B30 lysin was less effective. In a mouse model of bovine mastitis, both enzymes significantly reduced intramammary concentrations of all three streptococcal species (except for B30 vs. S. dysgalactiae), and the effects on mammary gland wet weights and TNF alpha concentrations were consistent with these findings. Unexpectedly, the synergistic effect determined for the two enzymes in vitro was not observed in the mouse model. Overall, our results illustrate the potential of endolysins for treatment of Streptococcus-induced bovine mastitis. C1 [Schmelcher, Mathias; Powell, Anne M.; Pohl, Calvin S.; Donovan, David M.] USDA ARS, Anim Biosci & Biotechnol Lab North East Area, Beltsville, MD 20705 USA. [Schmelcher, Mathias] ETH, Inst Food Nutr & Hlth, CH-8092 Zurich, Switzerland. [Camp, Mary J.] USDA ARS, Stat Grp, Off North East Area Director, Beltsville, MD 20705 USA. RP Schmelcher, M (reprint author), USDA ARS, Anim Biosci & Biotechnol Lab North East Area, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM mathias.schmelcher@hest.ethz.ch FU National Institutes of Health [1 R01 AI075077-01A1]; National Research Initiative [2007-35204-18395]; US State Department FX This work was supported in part by National Institutes of Health grant 1 R01 AI075077-01A1; National Research Initiative grant 2007-35204-18395 and US State Department funds supporting US-Pakistani and US-Russian collaborations. NR 69 TC 7 Z9 7 U1 6 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD OCT PY 2015 VL 99 IS 20 BP 8475 EP 8486 DI 10.1007/s00253-015-6579-0 PG 12 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CR6YA UT WOS:000361493200014 PM 25895090 ER PT J AU Zhou, ZG Hua, B Cao, XY Yang, J Olk, DC Deng, BL Liu, FJ Li, RY Mao, JD AF Zhou, Zhigao Hua, Bin Cao, Xiaoyan Yang, John Olk, Dan C. Deng, Baolin Liu, Fengjing Li, Renying Mao, Jingdong TI Chemical composition of dissolved organic matter from various sources as characterized by solid-state NMR SO AQUATIC SCIENCES LA English DT Article DE Dissolved organic matter (DOM); Chemical composition; Point/non-point source; Freshwater; Nuclear magnetic resonance (NMR) ID DIFFERENT LANDFILL AGES; MASS-SPECTROMETRY; HUMIC SUBSTANCES; WASTE-WATER; MOLECULAR CHARACTERIZATION; TEMPORAL VARIABILITY; LOWER MISSISSIPPI; SPECTROSCOPY; CARBON; LEACHATES AB This study used solid-state nuclear magnetic resonance techniques to characterize the chemical compositions of DOM from various sources and thereby explore the linkages of river DOM to its potential point and nonpoint sources. DOM samples were isolated from two streams draining watersheds of different land uses, landfill leachates of two ages, sequential (raw, facility- and wetland-treated) wastewaters in a wastewater treatment plant. Two sampling sites along the Missouri River were located upstream and downstream of the outlet of a tributary, to which the stream waters, landfill leachates, and wastewater effluents were discharged. Compared with the chemical composition of the stream DOM from a cropland-dominated watershed, the stream DOM from a grass/forestland-dominated watershed comprised 14 % more alkyl carbons (C), but similar to 5 % less O-alkyl C. The two leachate DOM samples from landfill cells of two ages showed similar area fractions of the carbon types, and their spectra exhibited a characteristic alkyl C peak at 37 ppm, potentially a molecular signature of the DOM of landfill leachate source. The DOM from the treated wastewater had slightly (similar to 4 %) less alkyl C than that from the raw wastewater, and the DOM from the wetland-treated wastewater displayed decreased O-alkyl C but increased alkyl C, both to a small degree (by similar to 4 %), compared to the DOM in the facility-treated wastewater. The chemical composition of DOM in the Missouri River was affected by the local organic inputs, with the DOM from downstream the outlet displaying similar to 11 % higher alkyl C. C1 [Zhou, Zhigao; Cao, Xiaoyan; Li, Renying; Mao, Jingdong] Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. [Hua, Bin; Yang, John; Liu, Fengjing] Lincoln Univ Missouri, Dept Agr & Environm Sci, Jefferson City, MO 65102 USA. [Olk, Dan C.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Deng, Baolin] Univ Missouri, Dept Civil & Environm Engn, Columbia, MO 65211 USA. [Zhou, Zhigao] Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Jiangsu, Peoples R China. [Li, Renying] Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Nanjing 210044, Jiangsu, Peoples R China. RP Mao, JD (reprint author), Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. EM jmao@odu.edu RI Cao, Xiaoyan/E-3492-2012 OI Cao, Xiaoyan/0000-0001-7571-6482 FU USDA-NIFA Capacity Building Grant Program [2010-38821-21558] FX The authors would like to thank Dr. Klaus Schmidt-Rohr at Brandeis University for his advice on NMR analysis and the manuscript. This project was funded by the USDA-NIFA Capacity Building Grant Program through the Grant No. 2010-38821-21558 to Lincoln University of Missouri, University of Missouri, and Old Dominion University. NR 46 TC 1 Z9 1 U1 6 U2 57 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 1015-1621 EI 1420-9055 J9 AQUAT SCI JI Aquat. Sci. PD OCT PY 2015 VL 77 IS 4 BP 595 EP 607 DI 10.1007/s00027-015-0405-8 PG 13 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CR5PY UT WOS:000361396800006 ER PT J AU Yamanaka, T Morimoto, N Nishida, GM Kiritani, K Moriya, S Liebhold, AM AF Yamanaka, Takehiko Morimoto, Nobuo Nishida, Gordon M. Kiritani, Keizi Moriya, Seiichi Liebhold, Andrew M. TI Comparison of insect invasions in North America, Japan and their Islands SO BIOLOGICAL INVASIONS LA English DT Article DE Biological invasion; Invasibility; Invasion pathway; Community composition; Non-native insects; Climatic similarities ID BIOLOGICAL-CONTROL; EUROPE; IMPACT; MODEL AB Among the various animal taxa that are have established outside their native ranges, invasions by insect species are the most numerous worldwide. In order to better understand the characteristics of insect species that make them more invasion prone, the characteristics of habitats that make them more susceptible to invasions and to understand the pathways that facilitate invasions, we compared the compositions of the native and non-native insect communities among five Pacific regions: North America, the Hawaiian Islands, mainland Japan, Ogasawara and Okinawa Islands. These regions comprise both island and mainland habitats and have historically been subjected to varying activities affecting insect movement. Among the five regions, the oceanic islands, Hawaii and Ogasawara, appear to be the most prone to invasions. Specific insect orders such as the Blattodea, Siphonaptera, Thysanoptera and Hemiptera are disproportionally represented in the non-native insect fauna compared to the native fauna in all regions. A large fraction of the non-native insect species in North America (9.4 %) and Hawaii (13.2 %) were introduced intentionally, as part of biological control programs, which were historically pursued more vigorously in those regions than in Japan. Dominance by individual insect orders within invaded communities can be explained by the historical importance of invasion pathways, some of which were likely similar among regions (e.g., movement on live plants), while other pathways appear more region specific. Translocation of insect species among world regions appears to be affected by climatic similarity, host plant similarity and propagule pressure, though these effects are often confounded. C1 [Yamanaka, Takehiko] Natl Inst Agroenvironm Sci, Nat Resources Inventory Ctr, Tsukuba, Ibaraki 3058604, Japan. [Morimoto, Nobuo] NARO Inst Livestock & Grassland Sci, Nasu Res Stn, Nasushiobara, Tochigi 3292793, Japan. [Nishida, Gordon M.] Univ Calif Berkeley, Berkeley, CA 94720 USA. [Moriya, Seiichi] Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan. [Liebhold, Andrew M.] USDA Forest Serv, No Res Stn, Morgantown, WV 26505 USA. RP Yamanaka, T (reprint author), Natl Inst Agroenvironm Sci, Nat Resources Inventory Ctr, 3-1-3 Kannondai, Tsukuba, Ibaraki 3058604, Japan. EM apple@affrc.go.jp FU National Socio-Environmental Synthesis Center pursuit project, "Globalization of the Live Plant Trade: Informing Efficient Strategies for Reducing Non-Native Pest Invasion Risk'' FX We thank R. Young and E. Luzader for assistance in compiling records. We also thank The Bernice Pauahi Bishop Museum (BPBM) for providing the newest Hawaiian list. This work was supported in part by the National Socio-Environmental Synthesis Center pursuit project, "Globalization of the Live Plant Trade: Informing Efficient Strategies for Reducing Non-Native Pest Invasion Risk''. NR 46 TC 5 Z9 5 U1 2 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD OCT PY 2015 VL 17 IS 10 BP 3049 EP 3061 DI 10.1007/s10530-015-0935-y PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR6MB UT WOS:000361460300020 ER PT J AU Leathers, TD Price, NPJ Bischoff, KM Manitchotpisit, P Skory, CD AF Leathers, Timothy D. Price, Neil P. J. Bischoff, Kenneth M. Manitchotpisit, Pennapa Skory, Christopher D. TI Production of novel types of antibacterial liamocins by diverse strains of Aureobasidium pullulans grown on different culture media SO BIOTECHNOLOGY LETTERS LA English DT Article DE Antibacterial; Aureobasidium pullulans; Exophilin; Heavy oil; Liamocin; Polyol lipids; Streptococcus antibacterial ID 5-HYDROXY-2-DECENOYL ESTERS; POLYOL LIPIDS; MANNITOL; 3,5-DIHYDROXYDECANOYL; ARABITOL; OILS AB Liamocins produced by strains of A. pullulans have potential agricultural and pharmaceutical applications as antibacterials with specificity against Streptococcus spp. Six strains of A. pullulans were characterized for liamocin production on four different culture media. The choice of strain and culture medium affected growth, liamocin yields, and production of contaminating pigments. Best growth and highest liamocin yields were obtained using A. pullulans strain NRRL 50384 grown on a sucrose basal medium. Unexpectedly, the choice of strain and culture medium also affected the structure of liamocins produced, providing novel types of liamocins. Liamocins varied not only in the ratios of trimer and tetramer polyester tail groups, but also in the nature of the polyol headgroup, which could include mannitol, arabitol, or glycerol. The ability to conveniently produce novel types of liamocins in good yields will provide novel antibacterials for applied uses, and facilitate structure-function studies on the mechanism of antibacterial activity. C1 [Leathers, Timothy D.; Price, Neil P. J.; Bischoff, Kenneth M.; Skory, Christopher D.] ARS, USDA, Natl Ctr Agr Utilizat Res, Renewable Prod Technol Res Unit,Agr Res Serv, Peoria, IL 61604 USA. [Manitchotpisit, Pennapa] Illinois State Univ, Sch Biol Sci, Normal, IL 61790 USA. RP Leathers, TD (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Renewable Prod Technol Res Unit,Agr Res Serv, 1815 N Univ St, Peoria, IL 61604 USA. EM tim.leathers@ars.usda.gov NR 17 TC 2 Z9 2 U1 3 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD OCT PY 2015 VL 37 IS 10 BP 2075 EP 2081 DI 10.1007/s10529-015-1892-3 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CR1FB UT WOS:000361068500021 PM 26112325 ER PT J AU Myers, MC Mason, JT Hoksch, BJ Cambardella, CA Pfrimmer, JD AF Myers, Mark C. Mason, James T. Hoksch, Benjamin J. Cambardella, Cynthia A. Pfrimmer, Jarrett D. TI Birds and butterflies respond to soil-induced habitat heterogeneity in experimental plantings of tallgrass prairie species managed as agroenergy crops in Iowa, USA SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE agricultural biodiversity; avian ecology; biofuel; community ecology; disturbance; edaphic variation; grassland restoration; habitat use; Lepidoptera; soil ecology ID COMMUNITY HETEROGENEITY; CONSERVATION; LANDSCAPE; DIVERSITY; MODELS; BIODIVERSITY; RESTORATION; BIOENERGY; SELECTION; FIRE AB The maintenance of habitat heterogeneity in agricultural landscapes has been promoted as a key strategy to conserve biodiversity. Animal response to grassland heterogeneity resulting from spatiotemporal variation in disturbance is well documented; however, the degree to which edaphic variation generates heterogeneity detectable by grassland wildlife has proven more difficult to study in natural settings. We conducted a field experiment to study how soils directly affect vegetation structure and composition and indirectly affect bird and butterfly assemblages using plantings of tallgrass prairie species managed as agroenergy crops in Iowa, USA. The experimental design included four vegetation treatments of varying species richness replicated on three soil types. Habitat characteristics varied widely among soils. Crops on sandy loam, the driest, most acidic soil with the lowest nutrient content, developed shorter, less dense vegetation with sparse litter accumulation and more bare ground compared to crops on loam and clay loam. Birds and butterflies responded similarly to soil-induced variation in habitat characteristics. Their abundance and species richness were similar on all soils, but their assemblage compositions varied among soils in certain vegetation treatments. In low-diversity grass crops, bird assemblages using sandy loam were dominated by species preferring open ground and sparse vegetation for foraging and nesting, whereas assemblages using loam and clay loam were dominated by birds preferring tall, dense vegetation with abundant litter. In high-diversity prairie crops, the species composition of forbs in bloom varied among soils and strongly influenced butterfly assemblages.Synthesis and applications. Prairie agroenergy crops established with identical management practices developed variable habitat characteristics due to natural edaphic variation, and this heterogeneity influenced the spatial distribution of bird and butterfly assemblages due to differential habitat use among species. This finding suggests that if unfertilized prairie crops were grown for agroenergy by land managers large-scale, soil-induced habitat heterogeneity would promote wildlife diversity within and among fields, further increasing the habitat value of these crops compared to the fertilized, annual monocultures that currently dominate the agricultural landscape. Our study also highlights the need for managers to consider soil properties when selecting sites to restore grassland habitat for species of conservation concern. Prairie agroenergy crops established with identical management practices developed variable habitat characteristics due to natural edaphic variation, and this heterogeneity influenced the spatial distribution of bird and butterfly assemblages due to differential habitat use among species. This finding suggests that if unfertilized prairie crops were grown for agroenergy by land managers large-scale, soil-induced habitat heterogeneity would promote wildlife diversity within and among fields, further increasing the habitat value of these crops compared to the fertilized, annual monocultures that currently dominate the agricultural landscape. Our study also highlights the need for managers to consider soil properties when selecting sites to restore grassland habitat for species of conservation concern. C1 [Myers, Mark C.; Mason, James T.; Hoksch, Benjamin J.] Univ No Iowa, Dept Biol, Cedar Falls, IA 50614 USA. [Mason, James T.; Hoksch, Benjamin J.] Univ No Iowa, Tallgrass Prairie Ctr, Cedar Falls, IA 50614 USA. [Cambardella, Cynthia A.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Pfrimmer, Jarrett D.] S Dakota State Univ, Dept Nat Resource Management, Brookings, SD 57007 USA. RP Myers, MC (reprint author), Univ No Iowa, Dept Biol, Cedar Falls, IA 50614 USA. EM mark.myers@uni.edu FU Iowa Power Fund; Iowa Science Foundation; Iowa EPSCoR (NSF) [EPS-1101284]; University of Northern Iowa FX We thank D. Smith, D. Williams and the Tallgrass Prairie Center staff for managing the site and providing productivity data; J. Ohmacht, G. Simmons, A. Morrow and the National Laboratory for Agriculture and the Environment staff for technical assistance; V. Fish and J. Wiemer of Black Hawk County Conservation Board for logistical support; and M. Sherrard, G. Siriwardena and three anonymous reviewers for critical reviews of the manuscript. Funding sources included the Iowa Power Fund, Iowa Science Foundation and Iowa EPSCoR (NSF Grant No. EPS-1101284). Summer fellowships provided to M.C.M. by the University of Northern Iowa facilitated our work. NR 46 TC 1 Z9 1 U1 9 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2015 VL 52 IS 5 BP 1176 EP 1187 DI 10.1111/1365-2664.12503 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR2UP UT WOS:000361186500008 ER PT J AU Wood, EM Pidgeon, AM Radeloff, VC Helmers, DP Culbert, PD Keuler, NS Flather, CH AF Wood, Eric M. Pidgeon, Anna M. Radeloff, Volker C. Helmers, David P. Culbert, Patrick D. Keuler, Nicholas S. Flather, Curtis H. TI Long-term avian community response to housing development at the boundary of US protected areas: effect size increases with time SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE avian abundance; avian richness; Breeding Bird Survey; inholding; private land; public land; species of greatest conservation need; synanthrope ID CONSERVATION DEVELOPMENT; UNITED-STATES; LAND-USE; EXURBAN DEVELOPMENT; HUMAN-POPULATION; BIODIVERSITY; PATTERNS; BIRDS; FRAGMENTATION; URBANIZATION AB Biodiversity conservation is a primary function of protected areas. However, protected areas also attract people, and therefore, land use has intensified at the boundaries of these lands globally. In the USA, since the 1970s, housing growth at the boundaries (<1km) of protected areas has increased at a rate far higher than on more distant private lands. Here, we designed our analyses to address our central hypothesis that increasing housing density in and near protected areas will increasingly alter their avian communities. We quantified the relationship between abundance and richness of protected-area avian species of greatest conservation need, land-cover affiliates (e.g. species associated with natural land cover such as forest breeders) and synanthropes (e.g. species associated with humans) with housing density on the boundary of protected areas and on more distant private lands from 1970 to 2010 in three ecoregions of the USA. We accomplished this using linear mixed-model analyses, data from the US Census Bureau and 90 routes of the North American Breeding Bird Survey. Housing density at the boundary of protected areas tended to be strongly negatively related with the abundance and richness of species of greatest conservation need and land-cover affiliates (upwards of 88% of variance explained) and strongly positively related with synanthropes (upwards of 83% of variance explained). The effect size of these relationships increased in most cases from 1970 to 2010 and was greatest in the densely developed eastern forests. In the more sparsely populated West, we found similar, though weaker, associations. Housing density on private lands more distant from protected areas had similar, but more muted negative effects.Synthesis and applications. Our results illustrate that as housing density has increased along the boundary of protected areas, the conservation benefit of these lands has likely diminished. We urge conservation planners to prioritize the purchase of private-land inholdings in order to maximize the extent of unfragmented natural lands within protected areas. Further, we strongly recommend that land-use planners implement boundary management strategies to alter the pattern of human access to protected areas, cluster development to concentrate the footprint of rural housing, and establish conservation agreements through local land trusts to buffer protected areas from the effects of development along protected-area boundaries. To maximize the conservation benefit of protected areas, we suggest that housing development should be restricted within 1km of their boundaries. Our results illustrate that as housing density has increased along the boundary of protected areas, the conservation benefit of these lands has likely diminished. We urge conservation planners to prioritize the purchase of private-land inholdings in order to maximize the extent of unfragmented natural lands within protected areas. Further, we strongly recommend that land-use planners implement boundary management strategies to alter the pattern of human access to protected areas, cluster development to concentrate the footprint of rural housing, and establish conservation agreements through local land trusts to buffer protected areas from the effects of development along protected-area boundaries. To maximize the conservation benefit of protected areas, we suggest that housing development should be restricted within 1km of their boundaries. C1 [Wood, Eric M.; Pidgeon, Anna M.; Radeloff, Volker C.; Helmers, David P.; Culbert, Patrick D.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Wood, Eric M.] Cornell Univ, Cornell Lab Ornithol, Ithaca, NY 14850 USA. [Keuler, Nicholas S.] Univ Wisconsin, Dept Stat, Madison, WI 53706 USA. [Flather, Curtis H.] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. RP Wood, EM (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM ericmwood@gmail.com RI Radeloff, Volker/B-6124-2016 OI Radeloff, Volker/0000-0001-9004-221X FU US Forest Service Rocky Mountain Research Station; NASA Biodiversity Program FX We gratefully acknowledge support for this research by the US Forest Service Rocky Mountain Research Station, and the NASA Biodiversity Program. We thank the volunteers who have collected BBS and housing census data, which have made this study possible. We thank M. Whittingham, R. Fuller and two anonymous reviewers for their very helpful comments to improve our manuscript. All authors declare no conflict of interests with this work. NR 48 TC 1 Z9 1 U1 7 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2015 VL 52 IS 5 BP 1227 EP 1236 DI 10.1111/1365-2664.12492 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR2UP UT WOS:000361186500013 ER PT J AU Duan, JJ Bauer, LS Abell, KJ Ulyshen, MD Van Driesche, RG AF Duan, Jian J. Bauer, Leah S. Abell, Kristopher J. Ulyshen, Michael D. Van Driesche, Roy G. TI Population dynamics of an invasive forest insect and associated natural enemies in the aftermath of invasion: implications for biological control SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE Hymenoptera; invasive; life table; natural enemies; parasitism; predation; wood borer ID EMERALD ASH BORER; AGRILUS-PLANIPENNIS COLEOPTERA; HYMENOPTERA-BRACONIDAE; NORTH-AMERICA; SEASONAL ABUNDANCE; BIOTIC FACTORS; BUPRESTIDAE; EULOPHIDAE; MICHIGAN; PARASITOIDS AB Understanding the population dynamics of exotic pests and associated natural enemies is important in developing sound management strategies in invaded forest ecosystems. The emerald ash borer (EAB) Agrilus planipennis Fairmaire is an invasive phloem-feeding beetle that has killed tens of millions of ash Fraxinus trees in North America since first detected in 2002. We evaluated populations of immature EAB life stages and associated natural enemies over a 7-year period (2008-2014) in six stands of eastern deciduous forest in southern Michigan, where Tetrastichus planipennisi Yang and two other Asian-origin EAB parasitoids were released for biological control between 2007 and 2010. We observed approximate to 90% decline in densities of live EAB larvae in infested ash trees at both parasitoid-release and control plots from 2009 to 2014 and found no significant differences in EAB density or mortality rates by parasitoids, avian predators or other undetermined factors between parasitoid-release and control plots. The decline in EAB larval density in our study sites was correlated with significant increases in EAB larval parasitism, first by native parasitoids, then by T.planipennisi. Life table analyses further indicated that parasitism by the introduced biocontrol agent and the North American native parasitoids contributed significantly to the reduction of net EAB population growth rates in our study sites from 2010 to 2014.Synthesis and applications. Our findings indicate that successful biocontrol of emerald ash borer (EAB) may involve suppression of EAB abundance both by local, generalist natural enemies (such as Atanycolus spp.) and by introduced specialist parasitoids (such as T.planipennisi). Biological control programmes against EAB in the aftermath of invasion should focus on establishing stable populations of T.planipennisi and other introduced specialist parasitoids for sustained suppression of low-density EAB populations. Moreover, we recommend releasing the introduced specialist biocontrol agents as soon as possible to prevent the outbreak of EAB populations in both newly infested and aftermath forests when EAB densities are still low. Our findings indicate that successful biocontrol of emerald ash borer (EAB) may involve suppression of EAB abundance both by local, generalist natural enemies (such as Atanycolus spp.) and by introduced specialist parasitoids (such as T.planipennisi). Biological control programmes against EAB in the aftermath of invasion should focus on establishing stable populations of T.planipennisi and other introduced specialist parasitoids for sustained suppression of low-density EAB populations. Moreover, we recommend releasing the introduced specialist biocontrol agents as soon as possible to prevent the outbreak of EAB populations in both newly infested and aftermath forests when EAB densities are still low. C1 [Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Bauer, Leah S.] US Forest Serv, USDA, No Res Stn, Lansing, MI 48910 USA. [Abell, Kristopher J.; Van Driesche, Roy G.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Ulyshen, Michael D.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Duan, JJ (reprint author), ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov FU USDA Forest Service, Forest Health Protection FX We thank Deborah Miller (USDA Forest Service) and many student helpers for assisting in laboratory work and field surveys of EAB larvae and their parasitoids. We are also grateful to Doug Luster (USDA Agricultural Research Service) for helpful comments on an early version of this manuscript, and Richard Reardon and Steven Katovich (USDA Forest Service, Forest Health Protection) for providing some financial support for this project. NR 43 TC 10 Z9 10 U1 16 U2 90 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2015 VL 52 IS 5 BP 1246 EP 1254 DI 10.1111/1365-2664.12485 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR2UP UT WOS:000361186500015 ER PT J AU Wang, XY Jennings, DE Duan, JJ AF Wang, Xiao-Yi Jennings, David E. Duan, Jian J. TI Trade-offs in parasitism efficiency and brood size mediate parasitoid coexistence, with implications for biological control of the invasive emerald ash borer SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE Agrilus planipennis; idiobiont ectoparasitoid; interspecific competition; invasive pest; koinobiont endoparasitoid; Spathius galinae; Tetrastichus planipennisi ID PLANIPENNISI HYMENOPTERA EULOPHIDAE; RUSSIAN FAR-EAST; COLEOPTERA BUPRESTIDAE; INTRINSIC COMPETITION; INTERSPECIFIC COMPETITION; ENDOPARASITOID WASPS; AGRILUS-PLANIPENNIS; LIFE-HISTORY; BRACONIDAE; RESOURCE AB Parasitoids often are selected for use as biological control agents because of their high host specificity, yet such host specificity can result in strong interspecific competition. Few studies have examined whether and how various extrinsic factors (such as parasitism efficiency, i.e. the ability to optimize host-finding attack rates) influence the outcome of competition between parasitoids, even though they could have profound effects on the implementation of classical biological control programmes. To determine the potential influence of extrinsic competition and coexistence on host suppression efficacy, we compared parasitism by two larval parasitoids (Tetrastichus planipennisi and Spathius galinae) of the invasive emerald ash borer (EAB) Agrilus planipennis, under different host densities, parasitoid densities, host plant sizes and parasitoid-host ratios.Spathius galinae had significantly higher parasitism efficiency (approximate to 4 times), but significantly lower brood size (>6 times) than that of T.planipennisi. The attack rates of hosts increased significantly with parasitoid density, whereas host density did not significantly affect multiparasitism. The parasitism rate of T.planipennisi on small host logs was significantly higher than that on large logs, while host plant (log) size had no significant impact on S.galinae parasitism. The multiparasitism rate was rather low regardless of host log size and parasitoid/host density, indicating that intrinsic competition between the two species of parasitoids might seldom occur in the field. The two species of parasitoids could therefore coexist in the same habitat, and any adverse effects on the suppression of EAB populations caused by competitive behaviour between the two species of parasitoids would likely be negligible.Synthesis and applications. Our findings suggest that introducing multiple species of parasitic natural enemies could be feasible for management of invasive species, but it is important to examine multiple extrinsic factors simultaneously when evaluating interspecific competition between them. Among these different extrinsic factors, we found that coexistence between parasitoids can be mediated by trade-offs in their parasitism efficiency and brood sizes. Thus, the differences in life-history traits of natural enemies could be used to select among biological control agents being considered for releases. Our findings suggest that introducing multiple species of parasitic natural enemies could be feasible for management of invasive species, but it is important to examine multiple extrinsic factors simultaneously when evaluating interspecific competition between them. Among these different extrinsic factors, we found that coexistence between parasitoids can be mediated by trade-offs in their parasitism efficiency and brood sizes. Thus, the differences in life-history traits of natural enemies could be used to select among biological control agents being considered for releases. C1 [Wang, Xiao-Yi] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China. [Jennings, David E.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Duan, Jian J.] ARS, Beneficial Insects Intro Res Unit, USDA, Newark, DE 19713 USA. RP Wang, XY (reprint author), Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China. EM xywang@caf.ac.cn OI Jennings, David/0000-0001-7179-3069 FU USDA Forest Service; China Scholarship Fund [201203270012] FX We are grateful to Jonathan Schmude, Kristi Larson, Susan Barth and Jackie Hoban for rearing of emerald ash borer and maintenance of parasitoids. This work was funded by the USDA Forest Service. Xiao-Yi Wang thanks the China Scholarship Fund (201203270012) for supporting his visit to USDA-ARS, BIIRU. We appreciate comments on an early draft of this manuscript by Doug Luster and Tim Watt, and we thank two anonymous reviewers for their valuable suggestions. NR 41 TC 4 Z9 4 U1 13 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2015 VL 52 IS 5 BP 1255 EP 1263 DI 10.1111/1365-2664.12487 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR2UP UT WOS:000361186500016 ER PT J AU Curtin, C Hubbard, K Anderson, SE Mick, E Must, A Bandini, LG AF Curtin, C. Hubbard, K. Anderson, S. E. Mick, E. Must, A. Bandini, L. G. TI Food Selectivity, Mealtime Behavior Problems, Spousal Stress, and Family Food Choices in Children with and without Autism Spectrum Disorder SO JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS LA English DT Article DE Autism spectrum disorder; Food selectivity; Mealtime behaviors ID TYPICALLY DEVELOPING-CHILDREN; FEEDING PROBLEMS; EATING BEHAVIORS; YOUNG-CHILDREN; DIETARY-INTAKE; QUESTIONNAIRE; ADOLESCENTS; VALIDATION; PATTERNS; QUALITY AB Mealtime behavior problems and family stress occur frequently among families of children with autism spectrum disorder (ASD). However, it is unknown whether food selectivity is an associated factor. The associations of high food selectivity with mealtime behavior problems, spousal stress, and influence on family members were assessed among 53 children with ASD and 58 typically developing (TD) children ages 3-11 years. Compared to TD children, children with ASD were more likely to have high food selectivity, and their parents reported more mealtime behavior problems, higher spousal stress, and influence on what other family members ate. High food selectivity was associated with mealtime behavior problems in both groups. Interventions to reduce food selectivity may lead to decreases in mealtime behavior problems. C1 [Curtin, C.; Bandini, L. G.] Univ Massachusetts, Sch Med, Eunice Kennedy Shriver Ctr, Charlestown, MA 02129 USA. [Hubbard, K.] Food & Nutr Serv, USDA, Western Reg Off, San Francisco, CA 94103 USA. [Anderson, S. E.] Ohio State Univ, Div Epidemiol, Coll Publ Hlth, Columbus, OH 43210 USA. [Mick, E.] Univ Massachusetts, Sch Med, Dept Quantitat Hlth Sci, Worcester, MA 01655 USA. [Must, A.] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. RP Curtin, C (reprint author), Univ Massachusetts, Sch Med, Eunice Kennedy Shriver Ctr, 465 Medford St,Suite 500, Charlestown, MA 02129 USA. EM carol.curtin@umassmed.edu; kristie.hubbard@fns.usda.gov; anderson.1767@osu.edu; eric.mick@umassmed.edu; aviva.must@tufts.edu; linda.bandini@umassmed.edu FU NIH [R21 HD048989, P30HD004147, P30DK046200]; Maternal and Child Health Research Program [UA3MC25735] FX This research was supported by the following NIH Grants: R21 HD048989, P30HD004147, and P30DK046200, and by a cooperative agreement with the Maternal and Child Health Research Program, Grant #UA3MC25735. NR 32 TC 5 Z9 5 U1 4 U2 27 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0162-3257 EI 1573-3432 J9 J AUTISM DEV DISORD JI J. Autism Dev. Disord. PD OCT PY 2015 VL 45 IS 10 BP 3308 EP 3315 DI 10.1007/s10803-015-2490-x PG 8 WC Psychology, Developmental SC Psychology GA CR6CF UT WOS:000361430200022 PM 26070276 ER PT J AU Naesset, E Bollandsas, OM Gobakken, T Solberg, S McRoberts, RE AF Naesset, Erik Bollandsas, Ole Martin Gobakken, Terje Solberg, Svein McRoberts, Ronald E. TI The effects of field plot size on model-assisted estimation of aboveground biomass change using multitemporal interferometric SAR and airborne laser scanning data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Biomass estimation; Biomass change estimation; ALS; Tandem-X; InSAR; Model-assisted estimation; Precision; Relative efficiency; Plot size; Plot boundary effect ID GROWING STOCK VOLUME; FOREST BIOMASS; LIDAR; INSAR; AREA; INVENTORY; INFERENCE; HEIGHT; DESIGN AB Remotely sensed data from airborne laser scanning (ALS) and interferometric synthetic aperture radar (InSAR) can greatly improve the precision of estimates of forest resource parameters such as mean biomass and biomass change per unit area. Field plots are typically used to construct models that relate the variable of interest to explanatory variables derived from the remotely sensed data. The models may then be used in combination with the field plots to provide estimates for a geographical area of interest with corresponding estimates of precision using model-assisted estimators. Previous studies have shown that field plot sizes found suitable for pure field surveys may be sub-optimal for use in combination with remotely sensed data. Plot boundary effects, co-registration problems, and misalignment problems favor larger plots because the relative impact of these effects on the models of relationships may decline by increasing plot size. In a case study in a small boreal forest area in southeastern Norway (852.6 ha) a probability sample of 145 field plots was measured twice over an 11 year period (1998/1999 and 2010). For each plot, field measurements were recorded for two plot sizes (200 m(2) and 300/400 m2). Corresponding multitemporal ALS (1999 and 2010) and InSAR data (2000 and 2011) were also available. Biomass for each of the two measurement dates as well as biomass change were modeled for all plot sizes separately using explanatory variables from the ALS and InSAR data, respectively. Biomass change was estimated using model-assisted estimators. Separate estimates were obtained for different methods for estimation of change, like the indirect method (difference between predictions of biomass for each of the two measurement dates) and the direct method (direct prediction of change). Relative efficiency (RE) was calculated by dividing the variance obtained for a pure field-based change estimate by the variance of a corresponding estimate using the model-assisted approach. For ALS, the RE values ranged between 7.5 and 15.0, indicating that approximately 7.5-15.0 as many field plots would be required for a pure field-based estimate to provide the same precision as an ALS-assisted estimate. For InSAR, RE ranged between 1.8 and 2.5. The direct estimation method showed greater REs than the indirect method for both remote sensing technologies. There was clearly a trend of improved RE of the model-assisted estimates by increasing plot size. For ALS and the direct estimation method RE increased from 9.8 for 200 m(2) plots to 15.0 for 400 m(2) plots. Similar trends of increasing RE with plot size were observed for InSAR. ALS showed on average 32-6.0 times greater RE values than InSAR. Because remote sensing can contribute to improved precision of estimates, sample plot size is a prominent design issue in future sample surveys which should be considered with due attention to the great benefits that can be achieved when using remote sensing if the plot size reflects the specific challenges arising from use of remote sensing in the estimation. That is especially the case in the tropics where field resources may be scarce and inaccessibility and poor infrastructure hamper field work. (C) 2015 Elsevier Inc. All rights reserved. C1 [Naesset, Erik; Bollandsas, Ole Martin; Gobakken, Terje] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, NO-1432 As, Norway. [Solberg, Svein] Norwegian Forest & Landscape Inst, NO-1431 As, Norway. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN USA. RP Naesset, E (reprint author), Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, POB 5003, NO-1432 As, Norway. EM erik.naesset@nmbu.no FU Research Council of Norway [184636/S30] FX This research has been funded by the Research Council of Norway (project #184636/S30: "Effects of changing climate on the alpine tree line and mountain forest carbon pools along 1500 km N-S and elevation gradients"). The authors would like to thank Mr. Arild Veidahl, one of the local forest owners who provided us with all necessary facilities to conduct the field inventories, and Mr. Vegard Lien of the Norwegian University of Life Sciences who provided technical assistance with the GPS work. Blom Geomatics, Norway, is acknowledged for collection and processing of the ALS data. NR 31 TC 5 Z9 7 U1 4 U2 21 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2015 VL 168 BP 252 EP 264 DI 10.1016/j.rse.2015.07.002 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CR5SY UT WOS:000361405500020 ER PT J AU Pastick, NJ Jorgenson, MT Wylie, BK Nield, SJ Johnson, KD Finley, AO AF Pastick, Neal J. Jorgenson, M. Torre Wylie, Bruce K. Nield, Shawn J. Johnson, Kristofer D. Finley, Andrew O. TI Distribution of near-surface permafrost in Alaska: Estimates of present and future conditions SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Alaska; Data mining; Pedometrics; Near-surface permafrost; Machine learning; Remote sensing ID NORTHERN BRITISH-COLUMBIA; HIGH-SPATIAL-RESOLUTION; ACTIVE-LAYER THICKNESS; REMOTELY-SENSED DATA; HUDSON-BAY LOWLANDS; YUKON RIVER-BASIN; CLIMATE-CHANGE; INTERIOR ALASKA; LAND-COVER; DISCONTINUOUS PERMAFROST AB High-latitude regions are experiencing rapid and extensive changes in ecosystem composition and function as the result of increases in average air temperature. Increasing air temperatures have led to widespread thawing and degradation of permafrost which in turn has affected ecosystems, socioeconomics, and the carbon cycle of high latitudes. Here we overcome complex interactions among surface and subsurface conditions to map near-surface permafrost through decision and regression tree approaches that statistically and spatially extend field observations using remotely sensed imagery, climatic data, and thematic maps of a wide range of surface and subsurface biophysical characteristics. The data fusion approach generated medium-resolution (30-m pixels) maps of near-surface (within 1 m) permafrost, active-layer thickness, and associated uncertainty estimates throughout mainland Alaska. Our calibrated models (overall test accuracy of similar to 85%) were used to quantify changes in permafrost distribution under varying future climate scenarios assuming no other changes in biophysical factors. Models indicate that near-surface permafrost underlies 38% of mainland Alaska and that near-surface permafrost will disappear on 16 to 24% of the landscape by the end of the 21st Century. Simulations suggest that near-surface permafrost degradation is more probable in central regions of Alaska than more northerly regions. Taken together, these results have obvious implications for potential remobilization of frozen soil carbon pools under warmer temperatures. Additionally, warmer and drier conditions may increase fire activity and severity, which may exacerbate rates of permafrost thaw and carbon remobilization relative to climate alone. The mapping of permafrost distribution across Alaska is important for land-use planning, environmental assessments, and a wide-array of geophysical studies. (C) 2015 Elsevier Inc. All rights reserved. C1 [Pastick, Neal J.] Stinger Ghaffarian Technol Inc, Sioux Falls, SD 57198 USA. [Jorgenson, M. Torre] Alaska Ecosci, Fairbanks, AK 99709 USA. [Wylie, Bruce K.] US Geol Survey, Earth Resources Observat & Sci Ctr, Sioux Falls, SD 57198 USA. [Nield, Shawn J.] Nat Resource Conservat Serv, USDA, Palmer, AK 99645 USA. [Johnson, Kristofer D.] US Dept Agr Forest Serv, No Res Stn, Newtown Sq, PA 19073 USA. [Finley, Andrew O.] Michigan State Univ, Dept Forestry & Geog, E Lansing, MI 48824 USA. [Pastick, Neal J.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. RP Pastick, NJ (reprint author), Stinger Ghaffarian Technol Inc, Sioux Falls, SD 57198 USA. EM njpastick@usgs.gov OI Wylie, Bruce/0000-0002-7374-1083 FU U.S. Geological Survey (USGS) Land Change Science Program; USGS [G08PC91508] FX Research was funded by the U.S. Geological Survey (USGS) Land Change Science Program. Work was performed under USGS contract G08PC91508. We thank 3 anonymous reviewers and Burke Minsley (USGS) for constructive comments that enhanced this manuscript. NR 114 TC 13 Z9 14 U1 7 U2 60 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2015 VL 168 BP 301 EP 315 DI 10.1016/j.rse.2015.07.019 PG 15 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CR5SY UT WOS:000361405500024 ER PT J AU Jiang, ZL Tang, Y Zhao, XM Zhang, MY Donovan, DM Tian, XC AF Jiang, Zongliang Tang, Yong Zhao, Xueming Zhang, Mingyuan Donovan, David M. Tian, Xiuchun (Cindy) TI Knockdown of Brm and Baf170, Components of Chromatin Remodeling Complex, Facilitates Reprogramming of Somatic Cells SO STEM CELLS AND DEVELOPMENT LA English DT Article ID EMBRYONIC STEM-CELLS; MAMMALIAN SWI/SNF COMPLEXES; SELF-RENEWAL; IPS CELLS; PLURIPOTENCY; GENE; ACTIVATION; STAT3; ESBAF; METHYLATION AB The SWI/SNF (SWItch/Sucrose NonFermentable or BAF, Brg/Brahma-associated factors) complexes are epigenetic modifiers of chromatin structure and undergo progressive changes in subunit composition during cellular differentiation. For example, in embryonic stem cells, esBAF contains Brg1 and Baf155, while their homologs, Brm and Baf170, are present in BAF of somatic cells. In this study, we sought to determine whether Brm and Baf170 play any roles in induced pluripotent stem cell (iPSC) reprogramming by using shRNA-mediated knockdown studies in the mouse model. We found that knocking down Brm during early, mid, and late stages (days 3, 6, and 9 after initial iPSC induction) and knocking down Baf170 during late-stage (day 9) reprogramming improve the numbers of iPSC colonies formed. We further showed that inhibition of these somatic BAF components also promotes complete reprogramming of partially reprogrammed somatic cells (pre-iPSCs). Finally, we found that the expression of Brm and Baf170 during reprogramming was regulated by Jak/Stat3 activity. Taken together, these data suggest that inhibiting somatic BAF improves complete reprogramming by facilitating the activation of the pluripotency circuitry. C1 [Jiang, Zongliang; Tang, Yong; Zhao, Xueming; Zhang, Mingyuan; Tian, Xiuchun (Cindy)] Univ Connecticut, Dept Anim Sci, Ctr Regenerat Biol, Storrs, CT 06269 USA. [Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD USA. RP Tian, XC (reprint author), Univ Connecticut, Dept Anim Sci, Ctr Regenerat Biol, 1390 Storrs Rd, Storrs, CT 06269 USA. EM xiuchun.tian@uconn.edu FU USDA [1265-31000-091-02S]; W2171 USDA Regional Project FX This project was supported by a USDA grant (1265-31000-091-02S) and the W2171 USDA Regional Project. NR 37 TC 2 Z9 2 U1 1 U2 8 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1547-3287 EI 1557-8534 J9 STEM CELLS DEV JI Stem Cells Dev. PD OCT 1 PY 2015 VL 24 IS 19 BP 2328 EP 2336 DI 10.1089/scd.2015.0069 PG 9 WC Cell & Tissue Engineering; Hematology; Medicine, Research & Experimental; Transplantation SC Cell Biology; Hematology; Research & Experimental Medicine; Transplantation GA CR5PP UT WOS:000361395900010 PM 26121422 ER PT J AU Kamfwa, K Cichy, KA Kelly, JD AF Kamfwa, Kelvin Cichy, Karen A. Kelly, James D. TI Genome-wide association analysis of symbiotic nitrogen fixation in common bean SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID PHASEOLUS-VULGARIS L; HOST-BACTERIA INTERACTIONS; N-2 FIXATION; NODULE NUMBER; SEED YIELD; LEGUMES; NODULATION; RHIZOBIUM; TRAITS; GROWTH AB Significant SNPs and candidate genes for symbiotic nitrogen fixation (SNF) and related traits were identified on Pv03, Pv07 and Pv09 chromosomes of common bean. A genome-wide association study (GWAS) was conducted to explore the genetic basis of variation for symbiotic nitrogen fixation (SNF) and related traits in the Andean Diversity Panel (ADP) comprising 259 common bean (Phaseolus vulgaris) genotypes. The ADP was evaluated for SNF and related traits in both greenhouse and field experiments. After accounting for population structure and cryptic relatedness, significant SNPs were identified on chromosomes Pv03, Pv07 and Pv09 for nitrogen derived from atmosphere (Ndfa) in the shoot at flowering, and for Ndfa in seed. The SNPs for Ndfa in shoot and Ndfa in seed co-localized on Pv03 and Pv09. Two genes Phvul.007G050500 and Phvul.009G136200 that code for leucine-rich repeat receptor-like protein kinases (LRR-RLK) were identified as candidate genes for Ndfa. LRR-RLK genes play a key role in signal transduction required for nodule formation. Significant SNPs identified in this study could potentially be used in marker-assisted breeding to accelerate genetic improvement of common bean for SNF. C1 [Kamfwa, Kelvin; Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Cichy, Karen A.] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. RP Kelly, JD (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM kellyj@msu.edu FU Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the Bureau for Economic Growth, Agriculture, and Trade, US Agency for International Development [EDH-A-00-07-00005-00]; US Department of Agriculture, Agricultural Research Service FX Research was supported by the Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the Bureau for Economic Growth, Agriculture, and Trade, US Agency for International Development, under the terms of Cooperative Agreement No. EDH-A-00-07-00005-00; and the US Department of Agriculture, Agricultural Research Service. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the US Agency for International Development or the US Government. We also thank Dr. Zixang Wen for his helpful comments on some aspects of data analyses. NR 60 TC 6 Z9 6 U1 7 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD OCT PY 2015 VL 128 IS 10 BP 1999 EP 2017 DI 10.1007/s00122-015-2562-5 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CR7QI UT WOS:000361545100008 PM 26133733 ER PT J AU Hwang, SD King, CA Ray, JD Cregan, PB Chen, P Carter, TE Li, ZL Abdel-Haleem, H Matson, KW Schapaugh, W Purcell, LC AF Hwang, Sadal King, C. Andy Ray, Jeffery D. Cregan, Perry B. Chen, Pengyin Carter, Thomas E., Jr. Li, Zenglu Abdel-Haleem, Hussein Matson, Kevin W. Schapaugh, William, Jr. Purcell, Larry C. TI Confirmation of delayed canopy wilting QTLs from multiple soybean mapping populations SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; MAX L.-MERR.; GLYCINE-MAX; DROUGHT TOLERANCE; COMPLEX TRAITS; GENETIC ARCHITECTURE; EXPERIMENTAL CROSSES; PLANT INTRODUCTION; UNITED-STATES; YIELD QTL AB QTLs for delayed canopy wilting from five soybean populations were projected onto the consensus map to identify eight QTL clusters that had QTLs from at least two independent populations. Quantitative trait loci (QTLs) for canopy wilting were identified in five recombinant inbred line (RIL) populations, 93705 KS4895 x Jackson, 08705 KS4895 x Jackson, KS4895 x PI 424140, A5959 x PI 416937, and Benning x PI 416937 in a total of 15 site-years. For most environments, heritability of canopy wilting ranged from 0.65 to 0.85 but was somewhat lower when averaged over environments. Putative QTLs were identified with composite interval mapping and/or multiple interval mapping methods in each population and positioned on the consensus map along with their 95 % confidence intervals (CIs). We initially found nine QTL clusters with overlapping CIs on Gm02, Gm05, Gm11, Gm14, Gm17, and Gm19 identified from at least two different populations, but a simulation study indicated that the QTLs on Gm14 could be false positives. A QTL on Gm08 in the 93705 KS4895 x Jackson population co-segregated with a QTL for wilting published previously in a Kefeng1 x Nannong 1138-2 population, indicating that this may be an additional QTL cluster. Excluding the QTL cluster on Gm14, results of the simulation study indicated that the eight remaining QTL clusters and the QTL on Gm08 appeared to be authentic QTLs. QTL x year interactions indicated that QTLs were stable over years except for major QTLs on Gm11 and Gm19. The stability of QTLs located on seven clusters indicates that they are possible candidates for use in marker-assisted selection. C1 [Hwang, Sadal; King, C. Andy; Chen, Pengyin; Purcell, Larry C.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Ray, Jeffery D.] USDA ARS, Crop Genet & Prod Res Unit, Stoneville, MS 38776 USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, BARC West, Beltsville, MD 20705 USA. [Carter, Thomas E., Jr.] N Carolina State Univ, USDA ARS, Dept Crop Sci, Raleigh, NC 27695 USA. [Li, Zenglu] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Li, Zenglu] Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Abdel-Haleem, Hussein] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Matson, Kevin W.] Monsanto Co, Global Soybean Breeding, St Louis, MO 63167 USA. [Schapaugh, William, Jr.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. RP Purcell, LC (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, 1366 Altheimer Dr, Fayetteville, AR 72704 USA. EM lpurcell@uark.edu FU United Soybean Board; United States Department of Agriculture-Agriculture Research Service (USDA-ARS) [6066-21220-012-00D] FX The authors gratefully acknowledge financial support for this research from the United Soybean Board. This work was also supported in part by the United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project number 6066-21220-012-00D. NR 61 TC 2 Z9 2 U1 5 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD OCT PY 2015 VL 128 IS 10 BP 2047 EP 2065 DI 10.1007/s00122-015-2566-1 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CR7QI UT WOS:000361545100011 PM 26163767 ER PT J AU Roberts, A Finardi, F Hegde, S Kiekebusch, J Klimpel, G Krieger, M Lema, MA Macdonald, P Nari, C Rubinstein, C Slutsky, B Vicien, C AF Roberts, Andrew Finardi-Filho, Flavio Hegde, Subray Kiekebusch, Juan Klimpel, Gonzalo Krieger, Mark Lema, Martin A. Macdonald, Philip Nari, Claudia Rubinstein, Clara Slutsky, Bernice Vicien, Carmen TI Proposed criteria for identifying GE crop plants that pose a low or negligible risk to the environment under conditions of low-level presence in seed SO TRANSGENIC RESEARCH LA English DT Review DE Genetically engineered plants; Low level presence; LLP; Environmental risk assessment; ERA AB The low-level presence (LLP) of genetically engineered (GE) seeds that have been approved in the country of origin but not the country of import presents challenges for regulators in both seed importing and exporting countries, as well as for the international seed trade and the farmers who rely on it. In addition to legal, financial and regulatory challenges, such LLP situations in seed may also require an environmental risk assessment by the country of import. Such assessments have typically been informed by the national framework established to support decisions related to wide scale cultivation, and frequently do not take into account the low environmental exposure and prior regulatory history of the GE plant. In addition, such assessment processes may not be well suited to the decision-making timeframe that is necessary when dealing with an LLP situation in imported seed. In order to facilitate regulatory decision making, this paper proposes a set of scientific criteria for identifying GE crop plants that are expected to pose a low or negligible risk to the environment under LLP conditions in seed. Regulatory decision makers in some importing countries may decide to use these criteria to assist in risk analysis associated with LLP situations they are experiencing or could experience in the future, and might choose to proactively apply the criteria to identify existing GE plants with regulatory approvals in other countries that would be expected to pose low risk under conditions of LLP in seed. C1 [Finardi-Filho, Flavio; Vicien, Carmen] ILSI Res Fdn, Ctr Environm Risk Assessment, Washington, DC USA. [Finardi-Filho, Flavio] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo, Brazil. [Hegde, Subray] USDA, Biotechnol Regulatory Serv, Anim & Plant Hlth Inspect Serv, Washington, DC USA. [Kiekebusch, Juan] Asociac Semilleros Argentinos, Buenos Aires, DF, Argentina. [Klimpel, Gonzalo] DuPont Pioneer, Johnston, IA USA. [Krieger, Mark] Dow AgroSci, Indianapolis, IN USA. [Lema, Martin A.] MAGyP, Buenos Aires, DF, Argentina. [Lema, Martin A.] Natl Univ Quilmes, Buenos Aires, DF, Argentina. [Macdonald, Philip] Canadian Food Inspect Agcy, Toronto, ON, Canada. [Nari, Claudia; Rubinstein, Clara] Monsanto Co, St Louis, MO USA. [Rubinstein, Clara] ILSI Argentina, Buenos Aires, DF, Argentina. [Slutsky, Bernice] Amer Seed Trade Assoc, Alexandria, VA USA. RP Roberts, A (reprint author), ILSI Res Fdn, Ctr Environm Risk Assessment, Washington, DC 20005 USA. EM aroberts@ilsi.org NR 9 TC 0 Z9 0 U1 5 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD OCT PY 2015 VL 24 IS 5 BP 783 EP 790 DI 10.1007/s11248-015-9899-z PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CR6LS UT WOS:000361459200001 PM 26264890 ER PT J AU Major, JE Mosseler, A Johnsen, KH Campbell, M Malcolm, J AF Major, John E. Mosseler, Alex Johnsen, Kurt H. Campbell, Moira Malcolm, John TI Red and black spruce provenance growth and allocation under ambient and elevated CO2 SO TREES-STRUCTURE AND FUNCTION LA English DT Article DE Biomass allocation; Black spruce; Elevated CO2; Fitness; Growth efficiency; Red spruce; Shade tolerance ID CARBON-DIOXIDE CONCENTRATION; CHLOROPLAST PIGMENT CONCENTRATIONS; DEVELOPMENTALLY ADAPTIVE TRAITS; SEED-SOURCE VARIATION; PICEA-RUBENS; GENETIC-VARIATION; ATMOSPHERIC CO2; NEW-ENGLAND; POPULATION VIABILITY; SHADE-TOLERANCE AB Late-successional red spruce performed relatively better than faster growing black spruce under elevated carbon dioxide; this may be related to species' shade tolerance. We compared growth, components of growth, and biomass allocation between two largely sympatric spruces, red spruce (Picea rubens Sarg.; RS) and black spruce (Picea mariana (Mill.) B.S.P.; BS) using 12 populations from across eastern Canada grown under ambient CO2 (aCO(2)) and elevated CO2 (eCO(2)) to evaluate absolute and relative responses to this increasing greenhouse gas. The 12 populations originated from continental to maritime environments, with seed collected from two proximate pairs of RS and BS stands from each of three provinces of eastern Canada-Ontario (ON), New Brunswick (NB), and Nova Scotia (NS)-considered here as provenances. Over 4 years, BS displayed increasingly greater height growth compared with RS. By the end of the 4th year, BS on average had 127 % greater total mass and 50 % greater root-collar diameter than RS. Bud flush was earlier for BS than RS, and BS bud flush occurred in the following order: ON, NB, NS. Under eCO(2), BS and RS allocated more mass to stem (stem + branches), and further analysis showed that 25 % of this difference in biomass allocation was attributable to increasing tree size, with the balance directly due to eCO(2). As expected, BS allocated more to aboveground than belowground mass than RS due their respective status as early- and late-successional species. However, under eCO(2), BS partitioned more belowground and RS more aboveground than under aCO(2). Black spruce had greater growth efficiency (GE; stem mass to needle mass ratio) than RS; GE increased with eCO(2), and the increase was greater for BS than RS. Elevated CO2 resulted in a relative biomass enhancement of 10.8 and 18.0 % for BS and RS, respectively. This does not support the theory that faster growing species will have a competitive advantage compared with inherently slower growing plants under eCO(2), but agrees with other observations that shade-tolerant species have greater response ratios to eCO(2) than shade-intolerant species. Also, despite high possibility of hybridization and introgression between the two species, particularly in the highly fragmented and small remnant populations from ON, the findings show that ON and NB remain true to species type for the major growth parameters, allometry, growth efficiencies, and physiological traits and that the provenance variation is mostly consistent with findings from common-garden studies. C1 [Major, John E.; Mosseler, Alex; Campbell, Moira; Malcolm, John] Atlant Forestry Ctr, Nat Resources Canada Canadian Forest Serv, Fredericton, NB E3B 5P7, Canada. [Johnsen, Kurt H.] USDA, Forest Serv, Southern Res Stn, Asheville, NC 28806 USA. RP Major, JE (reprint author), Atlant Forestry Ctr, Nat Resources Canada Canadian Forest Serv, 1350 Regent St, Fredericton, NB E3B 5P7, Canada. EM jmajor@nrcan.gc.ca FU Canadian Forest Service FX We gratefully acknowledge useful comments received from Bernie Daigle and Ed Swift. We gratefully acknowledge the greenhouse and growth-chamber growing skills of Laurie Yeates and Terry Hay, and the organizational and technical skills of Debby Barsi and Stephanie West are thankfully acknowledged. The financial support of the Canadian Forest Service is gratefully acknowledged. NR 60 TC 1 Z9 1 U1 5 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0931-1890 EI 1432-2285 J9 TREES-STRUCT FUNCT JI Trees-Struct. Funct. PD OCT PY 2015 VL 29 IS 5 BP 1313 EP 1328 DI 10.1007/s00468-015-1209-0 PG 16 WC Forestry SC Forestry GA CR6BZ UT WOS:000361429500003 ER PT J AU Eberle, KC Neill, JD Venn-Watson, SK McGill, JL Sacco, RE AF Eberle, Kirsten C. Neill, John D. Venn-Watson, Stephanie K. McGill, Jodi L. Sacco, Randy E. TI Novel Atlantic bottlenose dolphin parainfluenza virus TtPIV-1 clusters with bovine PIV-3 genotype B strains SO VIRUS GENES LA English DT Article DE Parainfluenza virus; Epithelial cells; Real-time PCR; Phylogeny; Growth kinetics; Pro-inflammatory cytokines ID PARAMYXOVIRUS V-PROTEINS; COMPLETE NUCLEOTIDE-SEQUENCE; RESPIRATORY SYNCYTIAL VIRUS; INTERFERON INDUCTION; TURSIOPS-TRUNCATUS; CONSERVED REGION; FUSION PROTEINS; HEPTAD REPEATS; MESSENGER-RNA; RIG-I AB Parainfluenza virus 3 (PIV-3) is a common viral infection not only in humans, but also in many other species. Serological evidence suggests that nearly 100 % of children in the United States have been infected with PIV-3 by 5 years of age. Similarly, in cattle, PIV-3 is commonly associated with bovine respiratory disease complex. A novel dolphin PIV-3 (TtPIV-1) was described by Nollens et al. in 2008 from a dolphin that was diagnosed with an unknown respiratory illness. At that time, TtPIV-1 was found to be most similar to, but distinct from, bovine PIV-3 (BPIV-3). In the present study, similar viral growth kinetics and pro-inflammatory cytokine (IL-1 beta, IL-6, and CXCL8) production were seen between BPIV-3 and TtPIV-1 in BEAS-2B, MDBK, and Vero cell lines. Initial nomenclature of TtPIV-1 was based on partial sequence of the fusion and RNA polymerase genes. Based on the similarities we saw with the in vitro work, it was important to examine the TtPIV-1 genome in more detail. Full genome sequencing and subsequent phylogenetic analysis revealed that all six viral genes of TtPIV-1 clustered within the recently described BPIV-3 genotype B strains, and it is proposed that TtPIV-1 be re-classified with BPIV-3 genotype B strains. C1 [Eberle, Kirsten C.; Neill, John D.; McGill, Jodi L.; Sacco, Randy E.] USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. [Eberle, Kirsten C.; Sacco, Randy E.] Iowa State Univ, Mol Cellular & Dev Biol Grad Program, Ames, IA USA. [Eberle, Kirsten C.; Sacco, Randy E.] Iowa State Univ, Immunobiol Grad Program, Ames, IA USA. [Venn-Watson, Stephanie K.] Natl Marine Mammal Fdn, Translat Med & Res Program, San Diego, CA USA. RP Sacco, RE (reprint author), USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, POB 70, Ames, IA 50010 USA. EM randy.sacco@ars.usda.gov FU Office of Naval Research [N0001412IP20029] FX We thank James Wellehan at the University of Florida Marine Mammal Health Program for providing us the TtPIV-1 isolate. We would also like to thank Judith Stasko in the Microscopy Services Unit at the National Animal Disease Center, Ames, IA for help with the electron microscopy portion. Funding for this project was supported in part by the Office of Naval Research Grant N0001412IP20029. NR 53 TC 1 Z9 1 U1 1 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD OCT PY 2015 VL 51 IS 2 BP 198 EP 208 DI 10.1007/s11262-015-1224-7 PG 11 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA CR6LU UT WOS:000361459400004 PM 26174699 ER PT J AU Nicholls, D Bumgardner, M AF Nicholls, David Bumgardner, Matthew TI Low-grade and character-marked hardwoods: A research review and synthesis of solid wood manufacturing and marketing SO FORESTRY CHRONICLE LA English DT Article DE low-grade hardwoods; character-marks; value-added hardwoods; industrial wood use; product design; small-diameter timber; wood perception ID RED-OAK LOGS; NORTHEASTERN UNITED-STATES; SMALL-DIAMETER HARDWOOD; LUMBER YIELD INCREASE; PALLET PART YIELDS; HOUSEHOLD FURNITURE; CONSUMER PREFERENCES; ECONOMIC-FEASIBILITY; PRODUCT DESIGN; WEST-VIRGINIA AB There is a substantial body of research from the past half-century addressing hardwood utilization and markets in North America. This synthesis is the first to our knowledge to consider two major (and related) aspects of this research concurrently: low-grade hardwood utilization and marketing of character-marked wood features. We first consider low-grade hardwood resources, products, and key challenges in processing and utilization researched since the 1970s. We then discuss several themes influencing marketing of character-mark products, including product development and consumer and retailer response. This review (considering 119 scientific papers) should help guide future research and value-added utilization of low-grade hardwoods, as it identifies important research results, needs, and gaps yet to be filled. These findings are important in an era of structural changes in the North American hardwood industry and increased pressure to maximize economic value from all hardwood resources. C1 [Nicholls, David] US Forest Serv, Pacific Northwest Res Stn, Sitka, AK 99835 USA. [Bumgardner, Matthew] US Forest Serv, Northern Res Stn, Delaware, OH USA. RP Nicholls, D (reprint author), US Forest Serv, Pacific Northwest Res Stn, Sitka, AK 99835 USA. EM dlnicholls@fs.fed.us NR 119 TC 0 Z9 0 U1 0 U2 0 PU CANADIAN INST FORESTRY PI MATTAWA PA C/O CANADIAN ECOLOGY CENTRE, PO BOX 430, 6905 HWY 17 W, MATTAWA, ONTARIO P0H 1V0, CANADA SN 0015-7546 EI 1499-9315 J9 FOREST CHRON JI For. Chron. PD OCT PY 2015 VL 91 IS 5 BP 548 EP 559 DI 10.5558/tfc2015-094 PG 12 WC Forestry SC Forestry GA V46JE UT WOS:000209879700014 ER PT J AU Han, M Zhao, CY Simunek, J Feng, G AF Han, Ming Zhao, Chengyi Simunek, Jirka Feng, Gary TI Evaluating the impact of groundwater on cotton growth and root zone water balance using Hydrus-1D coupled with a crop growth model SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Cotton; Groundwater; Yield; Evapotranspiration; Hydrus-1D; Crop growth ID MULCHED DRIP IRRIGATION; SOIL-SALINITY; NORTHWEST CHINA; MOISTURE; YIELD; L. AB Groundwater is an important factor that needs to be considered when evaluating the water balance of the soil-plant-atmosphere system and the sustainable development of arid oases. However, the impact of shallow groundwater on the root zone water balance and cotton growth is not fully understood. In this study, we have first analyzed the influence of the groundwater table depth on the seasonal maximum leaf area index of cotton, the average seasonal water stress, cotton yield, actual transpiration, actual evaporation, and capillary rise using experimental data collected at the Aksu water balance station, in Xinjiang, northwest of China and the Hydrus-1D variably-saturated soil water flow model coupled with a simplified crop growth model from SWAT. The coupled model has been first calibrated and validated using field observations of soil water content, leaf area index, cotton height, the above ground biomass, and cotton yield comparisons between measured and modeled variables have shown a reasonable agreement for all variables. Additionally, with a validated model, we have carried out numerical experiments from which we have concluded that groundwater is a major water resource for cotton growth in this region. The capillary rise from groundwater contributes almost 23% of crop transpiration when the average groundwater depth is 1.84 m, which is the most suitable groundwater depth for this experimental site. We have concluded that cotton growth and various components of the soil water balance are highly sensitive to the groundwater table level. Different positions of the groundwater table showed both positive and negative effects on cotton growth. Likewise, cotton growth has a significant impact on the capillary rise from groundwater. As a result, groundwater is a crucial factor that needs to be considered when evaluating agricultural land management in this arid region. The updated Hydrus-1D model developed in this study provides a powerful modeling tool for evaluating the effects of the groundwater table on local land management. (C) 2015 Elsevier B.V. All rights reserved. C1 [Han, Ming; Zhao, Chengyi] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Oasis Desert & Ecol, Urumqi 830011, Peoples R China. [Han, Ming] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Simunek, Jirka] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92506 USA. [Feng, Gary] ARS, Genet & Sustainable Agr Res Unit, USDA, Mississippi State, MS 39762 USA. RP Zhao, CY (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Oasis Desert & Ecol, Urumqi 830011, Peoples R China. EM zcy@ms.xjb.ac.cn FU Key National Natural Science Foundation [U14003281, 4117095]; National Project of China [2013BAC10B01]; SuMaRiO of BMBF (German Federal Ministry of Science and Technology) FX The study was supported by the Key National Natural Science Foundation (U14003281), (4117095), National Project (No. 2013BAC10B01) of China and Project SuMaRiO of BMBF (German Federal Ministry of Science and Technology) - Sustainable Management of River Oases along the Tarim River. The authors are grateful to Dr. Markus Disse and Keilholz Patrick of Technical University Munich, Germany, for their help. NR 29 TC 4 Z9 5 U1 10 U2 70 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD OCT PY 2015 VL 160 BP 64 EP 75 DI 10.1016/j.agwat.2015.06.028 PG 12 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CQ8NL UT WOS:000360864900007 ER PT J AU Zhang, HB Richardson, PA Belayneh, BE Ristvey, A Lea-Cox, J Copes, WE Moorman, GW Hong, CX AF Zhang, Haibo Richardson, Patricia A. Belayneh, Bruk E. Ristvey, Andrew Lea-Cox, John Copes, Warren E. Moorman, Gary W. Hong, Chuanxue TI Characterization of water quality in stratified nursery recycling irrigation reservoirs SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Recycling irrigation reservoir; Stratification; Water quality; Electrical conductivity; Oxidation reduction potential; Chlorophyll a ID AQUATIC ECOSYSTEMS; RUNOFF; PHYTOPLANKTON; MANAGEMENT; SIMULATION; NUTRIENTS; WETLANDS; REMOVAL; SHALLOW; PH AB Recycling irrigation reservoirs (RIRs) are an emerging aquatic ecosystem of global significance. Building upon the recent discovery of thermal stratification in these novel systems and its impacts on the vertical distribution of dissolved oxygen and pH, this study investigated the dynamics of chlorophyll a (CHLA), oxidation reduction potential (ORP), electrical conductivity (EC), and turbidity in the water columns of eight RIRs in the Mid-Atlantic region in USA over a 3-year period from 2011 to 2014. The vertical distribution of CHLA was associated with thermal stratification while ORP and EC were directly affected by thermal stratification. Specifically, CHLA concentration was greatest in the middle thermocline, followed by lower hypolimnion during the Stratification period. ORP level was higher at the surface than bottom with differences up to 505.67 my in Reservoir VA23. EC level was higher in the bottom than at the surface with the maximum difference of 0.90 dS m(-1) in Reservoir VA22. The top-bottom ORP and EC differences increased when water was stratified. Turbidity was indirectly affected by stratification. The correlation of turbidity with total precipitation was stronger during the non-stratification period from November to March than during the stratification period from April to October, mainly due to restriction on water mixing. The characterization of these additional water quality parameters in the water columns of RIRs provides fundamental knowledge about this novel water system and will help in developing sound water quality management practices for improved crop health and production while reducing agriculture's environmental footprint. This study also provides additional evidence supporting pH as a significant indicator for monitoring water quality in RIRs. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhang, Haibo; Richardson, Patricia A.; Hong, Chuanxue] Virginia Polytech Inst & State Univ, Hampton Rd Agr Res & Extens Ctr, Virginia Beach, VA 23455 USA. [Belayneh, Bruk E.; Ristvey, Andrew; Lea-Cox, John] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Copes, Warren E.] ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. [Moorman, Gary W.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. RP Zhang, HB (reprint author), Virginia Polytech Inst & State Univ, Hampton Rd Agr Res & Extens Ctr, 1444 Diamond Springs Rd, Virginia Beach, VA 23455 USA. EM haiboz85@vt.edu FU Virginia Agricultural Experiment Station; USDA National Institute of Food and Agriculture Specialty Crop Research Initiative [2010-51181-21140]; Hatch Program FX The data for this paper can be accessed at http://hdl.handle.net/10919/50561 of VTechWorks at Virginia Polytechnic Institute and State University. This research was supported in part by Virginia Agricultural Experiment Station, the USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (Agreement number 2010-51181-21140) and Hatch Program. We thank collaborating growers for providing access to the study reservoirs and timely on-site assistances. NR 32 TC 0 Z9 0 U1 3 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD OCT PY 2015 VL 160 BP 76 EP 83 DI 10.1016/j.agwat.2015.06.027 PG 8 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CQ8NL UT WOS:000360864900008 ER PT J AU Kurtzman, CP AF Kurtzman, Cletus P. TI Description of Martiniozyma gen. nov and transfer of seven Candida species to Saturnispora as new combinations SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Martiniozyma gen. nov.; Saturnispora; New yeast species combinations ID PHYLOGENETIC-RELATIONSHIPS; SEQUENCE-ANALYSIS; YEASTS; GENERA; WILLIOPSIS; THAILAND AB DNA sequence analysis has shown Candida abiesophila (NRRL Y-11514(T), CBS 5366(T)) and Candida asiatica (NRRL Y-63747(T), CBS 10863(T)) to be members of a small clade that is phylogenetically separate from other yeasts. In view of their isolation from neighboring genera, such as Pichia and Saturnispora, the two anamorphic species are proposed for transfer to Martiniozyma gen. nov. (MycoBank MB 812061) with Martiniozyma abiesophila designated as type species (MycoBank MB 812062). In keeping with the International Code of Nomenclature for algae, fungi, and plants, which specifies that related anamorphic and teleomorphic species can be assigned to the same genus, the following Candida species are transferred to Saturnispora to conform with their phylogenetic placement: Candida diversa (NRRL Y-5713(T)), Candida halmiae (CBS 11009(T)), Candida sanitii (CBS 10864(T)), Candida sekii (CBS 10931(T)), Candida siamensis (CBS 11022(T)), Candida silvae (NRRL Y-6725(T)) and Candida suwanaritii (CBS 11021(T)). C1 ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Kurtzman, CP (reprint author), ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov NR 24 TC 4 Z9 4 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD OCT PY 2015 VL 108 IS 4 BP 803 EP 809 DI 10.1007/s10482-015-0536-x PG 7 WC Microbiology SC Microbiology GA CQ8NG UT WOS:000360864000001 PM 26188713 ER PT J AU Uselman, SM Snyder, KA Leger, EA Duke, SE AF Uselman, Shauna M. Snyder, Keirith A. Leger, Elizabeth A. Duke, Sara E. TI Emergence and early survival of early versus late seral species in Great Basin restoration in two different soil types SO APPLIED VEGETATION SCIENCE LA English DT Article DE Cheatgrass (Bromus tectorum); Ecological resistance; Functional traits; Medusahead (Taeniatherum caput-medusae); Niche overlap; Plant-plant interactions; Seedling emergence and survival; Soil-plant relationships ID SAGEBRUSH STEPPE; COMMUNITY INVASIBILITY; GRASSLAND RESTORATION; PLANT INVASION; ESTABLISHMENT; COMPETITION; RESISTANCE; GRASSES; ECOSYSTEMS; CHEATGRASS AB QuestionsHow does performance of a native early seral seed mix during early life stages compare to that of a late seral mix when seeded with Bromus tectorum or Taeniatherum caput-medusae? Does either mix reduce survival of exotic annual grasses during early life stages, and does this effect differ with soil type? Is exotic performance stronger in one soil type, and does native performance when growing with exotics differ between soil types as a result of exotic preference? LocationUniversity of Nevada Agricultural Research Station, Reno, NV, US. MethodsWe compared seedling emergence and early survival (Nov through May) of two native seed mixes, each composed of grass, forb and shrub functional groups, when growing with B.tectorum or T.caput-medusae in soils of contrasting texture (sandy loam and clay loam). Natives were also seeded without exotics. Performance of an early seral mix was compared with that of a late seral mix representative of species commonly used in Great Basin restoration. ResultsComparing emergence and survival probabilities, early seral natives generally outperformed late seral natives when growing with exotics and had earlier emergence timing, although results differed among functional groups and soil types. In contrast, survival probabilities did not differ between the early and late seral mixes when growing without exotics. Within each seed mix, native grasses exhibited the highest emergence probabilities of the functional groups. Natives did not suppress exotics in early life stages. Performance of B.tectorum was higher on sandy loam, while T.caput-medusae was highly successful in both soil types. Performance of native functional groups differed by soil type when growing with exotics but did not differ when growing without exotics. Survival of native grasses, in particular, was generally higher on sandy loam when growing with exotics. ConclusionsFindings suggest that the use of early seral natives in areas invaded by exotic annual grasses may improve early seedling survival in the Great Basin, in comparison to the use of later seral species in traditional seed mixes. Variation in native performance in the two soil types may be helpful in tailoring seed mixes to different sites. C1 [Uselman, Shauna M.; Snyder, Keirith A.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. [Leger, Elizabeth A.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Duke, Sara E.] USDA ARS, Southern Plains Area Off, College Stn, TX 77845 USA. RP Uselman, SM (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. EM s.uselman@sbcglobal.net; keirith.snyder@ars.usda.gov; eleger@cabnr.unr.edu; sara.duke@ars.usda.gov FU Great Basin Native Plant Selection and Increase Project, U.S. Forest Service FX This research was partially supported by the Great Basin Native Plant Selection and Increase Project, U.S. Forest Service, and Nancy Shaw, who made the completion of this project possible. Research was conducted at the Nevada Agricultural Experiment Station, University of Nevada-Reno (UNR), and we thank Bo Kindred. We thank Dr. T. Forbis for contributing to the initial design of this study, and Dr. E. Goergen for many helpful conversations. We appreciate the assistance of S. Lencioni and S. Li in data collection; C. Bacher, M. Newell, A. Smith, M. Stout, B. Wehan and J. Whipple also helped collect data. S. Swim, B. Raitter, T. Jones, D. Harmon and T. Bobo assisted with seed and/or soil collection. T. Jones, C. Clements, S. Li, B. Raitter and D. Harmon helped in implementing the common garden. We thank A. J. Tiehm of the UNR Herbarium for verifying plant identification. We also thank Dr. P.S.J. Verburg, the Editor and anonymous referees for thoughtful comments for improving an earlier version of this manuscript. NR 58 TC 0 Z9 0 U1 9 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD OCT PY 2015 VL 18 IS 4 BP 624 EP 636 DI 10.1111/avsc.12175 PG 13 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CR2SU UT WOS:000361181500009 ER PT J AU Andersen, KM Naylor, BJ Endress, BA Parks, CG AF Andersen, Kelly M. Naylor, Bridgett J. Endress, Bryan A. Parks, Catherine G. TI Contrasting distribution patterns of invasive and naturalized non-native species along environmental gradients in a semi-arid montane ecosystem SO APPLIED VEGETATION SCIENCE LA English DT Article DE Community assembly; Disturbance; Elevation gradient; Functional groups; Habitat filtering; Non-native species; Plant invasions; Species turnover ID PLANT INVASIONS; ELEVATIONAL GRADIENTS; BIOLOGICAL INVASIONS; CLIMATE-CHANGE; UNITED-STATES; LAND-USE; ROADS; COMMUNITIES; FOREST; RESTORATION AB QuestionsMountain systems have high abiotic heterogeneity over local spatial scales, offering natural experiments for examining plant species invasions. We ask whether functional groupings explain non-native species spread into native vegetation and up elevation gradients. We examine whether non-native species distribution patterns are related to environmental variables after controlling for elevation and, thus, driven by niche processes. LocationThe Wallowa Mountains, northeast Oregon, USA. MethodsWe surveyed non-native plant species along three mountain roads and into the native habitat matrix to assess the extent of invasion success along distance from roadside and elevation gradients. We used GLM to predict single species occurrence probabilities, LMM to examine differences in distribution patterns among functional types, and pCCA to examine multivariate responses of the non-native community to ecological variables. ResultsProbability of occurrence of the eight focal invasive species was not significantly related to distance from the road, but declined with elevation. Non-native species with annual life history strategies were more restricted to lower elevations than perennial species. Non-native species considered invasive occurred at lower minimum elevations than naturalized species. Shifts in the species composition of the non-native plant community were related to changes in soil and climate variables. ConclusionsOur results suggest that invasive species have similar patterns of habitat associations and spread from roadsides to interior vegetation zones, whereas naturalized species partition environmental gradients in this semi-arid montane ecosystem. Furthermore, annual and invasive species groups occupy lower elevations and perennial and naturalized species groups have invaded further up the mountain roads and into the native vegetation. Thus, functional groupings may explain contrasting distribution patterns of non-native species and could be used to inform management strategies for non-native species. C1 [Andersen, Kelly M.] Univ Exeter, Dept Geog, Exeter EX4 4RJ, Devon, England. [Naylor, Bridgett J.; Parks, Catherine G.] US Forest Serv, USDA, Pacific NW Res Stn, La Grande, OR 97850 USA. [Andersen, Kelly M.; Endress, Bryan A.] San Diego Zoo Global, Inst Conservat Res, Div Appl Plant Ecol, Escondido, CA 92027 USA. [Endress, Bryan A.] Oregon State Univ, Eastern Oregon Agr & Nat Resource Program, La Grande, OR 97850 USA. RP Andersen, KM (reprint author), Univ Exeter, Dept Geog, Rennes Dr,Amory Bldg, Exeter EX4 4RJ, Devon, England. EM kanderse@life.illinois.edu; bnaylor@fs.fed.us; Bryan.endress@oregonstate.edu; cparks01@fs.fed.us FU USFS Pacific Northwest Research Station; National Fire Plan program; Mountain Invasion Research Network (MIREN) FX We would like to thank Susan Geer, Kent Coe and the entire field crew for their expertise in the field, as well as two anonymous reviewers for proving valuable comments improving this manuscript. Support for this research was partially provided by USFS Pacific Northwest Research Station and the National Fire Plan program in collaboration with the Mountain Invasion Research Network (MIREN). MIREN is an international research consortium focused on understanding the effects of global change on plant invasions and plant biodiversity in mountainous areas (http://www.miren.ethz.ch/). NR 44 TC 2 Z9 2 U1 6 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD OCT PY 2015 VL 18 IS 4 BP 683 EP 693 DI 10.1111/avsc.12185 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CR2SU UT WOS:000361181500014 ER PT J AU Hernandez-Stefanoni, JL Johnson, KD Cook, BD Dupuy, JM Birdsey, R Peduzzi, A Tun-Dzul, F AF Luis Hernandez-Stefanoni, Jose Johnson, Kristofer D. Cook, Bruce D. Manuel Dupuy, Juan Birdsey, Richard Peduzzi, Alicia Tun-Dzul, Fernando TI Estimating species richness and biomass of tropical dry forests using LIDAR during leaf-on and leaf-off canopy conditions SO APPLIED VEGETATION SCIENCE LA English DT Article DE Above-ground biomass; Canopy conditions; Forest structure; Species richness; LIDAR; Topography; Tropical dry forest ID AIRBORNE LIDAR; YUCATAN PENINSULA; ALPHA-DIVERSITY; SCALE VARIATION; REGIONAL-SCALE; TREE HEIGHT; CARBON; MEXICO; DENSITY; SLOPE AB QuestionsIs the accuracy of predictions of above-ground biomass (AGB) and plant species richness of tropical dry forests from LIDAR data compromised during leaf-off canopy period, when most of the vegetation is leafless, compared to the leaf-on period? How does topographic position affect prediction accuracy of AGB for leaf-off and leaf-on canopy conditions? LocationTropical dry forest, Yucatan Peninsula, Mexico. MethodsWe evaluated the accuracy of predictions using both leaf-on and leaf-off LIDAR estimates of biomass and species richness, and assessed the adequacy of both LIDAR data sets for characterizing these vegetation attributes in tropical dry forests using multiple regression analysis and ANOVA. The performance of the models was assessed by leave-one-out cross-validation. We also investigated differences in vegetation structure between two topographic conditions using PCA and ANOSIM. Finally, we evaluated the influence of topography on the accuracy of biomass estimates from LIDAR using multiple regression analysis and ANOVA. ResultsA higher overall accuracy was obtained with leaf-on vs leaf-off conditions for AGB (root mean square error (RMSE)=21.6 vs 25.7tonha(-1)), as well as for species richness (RMSE=5.5 vs 5.8 species, respectively). However, no significant differences in mean dissimilarities between biomass estimates from LIDAR and in situ biomass estimates comparing the two canopy conditions were found (F-1,F-39=0.03, P=0.87). In addition, no significant differences in dissimilarities of AGB estimation were found between flat and hilly areas (F-1,F-39=1.36, P=0.25). ConclusionsOur results suggest that estimates of species richness and AGB from LIDAR are not significantly influenced by canopy conditions or slope, indicating that both leaf-on and leaf-off models are appropriate for these variables regardless of topographic position in these tropical dry forests. C1 [Luis Hernandez-Stefanoni, Jose; Manuel Dupuy, Juan; Tun-Dzul, Fernando] Ctr Invest Cient Yucatan AC, Unidad Recursos Nat, Merida 97200, Yucatan, Mexico. [Johnson, Kristofer D.; Birdsey, Richard; Peduzzi, Alicia] US Forest Serv, No Res Stn, Newtown Sq, PA 19073 USA. [Cook, Bruce D.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. RP Hernandez-Stefanoni, JL (reprint author), Ctr Invest Cient Yucatan AC, Unidad Recursos Nat, Calle 43 130, Merida 97200, Yucatan, Mexico. EM jl_stefanoni@cicy.mx; kristoferdjohnson@fs.fed.us; bruce.cook@nasa.gov; jmdupuy@cicy.mx; rbirdsey@fs.fed.us; apeduzzi@fs.fed.us; fjtun@cicy.mx FU Reinforcing REDD+ and South-South Cooperation Project - Mexican National Forest Commission (CONAFOR); United States Forest Service (USFS); USAID FX We are grateful to Kaxil Kiuic A. C. for logistic support, and Filogonio May-Pat and Manuela Tamayo for assistance with fieldwork and data processing. We also thank two anonymous reviewers and the editor, whose constructive and insightful comments helped improve the clarity and impact of this paper. The study was financially supported by the Reinforcing REDD+ and South-South Cooperation Project, funded by the Mexican National Forest Commission (CONAFOR), United States Forest Service (USFS) and USAID. This study is part of the Mexican network of Intensive Carbon Monitoring Sites (MEX-SMIC), an affiliation of academic institutions, government and NGOs with various funding sources. The network currently includes five forested sites located in strategic landscapes for REDD+ activities in Mexico. NR 48 TC 2 Z9 2 U1 7 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD OCT PY 2015 VL 18 IS 4 BP 724 EP 732 DI 10.1111/avsc.12190 PG 9 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CR2SU UT WOS:000361181500018 ER PT J AU Lightle, D Dossett, M Ebert, T Finn, CE Martin, RR Lee, JC AF Lightle, D. Dossett, M. Ebert, T. Finn, C. E. Martin, R. R. Lee, J. C. TI Effects of three novel resistant black raspberry selections on Amphorophora agathonica feeding behavior and performance SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Aphid resistance; Electrical penetration graph; EPG; Plant acceptance; Rubus spp.; Feeding behavior ID PENETRATION GRAPH DATA; MYZUS-PERSICAE; RED RASPBERRY; VIRUS TRANSMISSION; APHIS-GOSSYPII; DWARF-VIRUS; APHIDIDAE; IDENTIFICATION; HOMOPTERA; HEMIPTERA AB Host plant resistance is a practical and cost-effective approach for growers to manage insect pests. Recently, three new sources of resistance in black raspberry (Rubus occidentalis; selections ORUS 3778-1, ORUS 3817-1, and ORUS 4109-1) against the large raspberry aphid, Amphorophora agathonica, were identified. We studied stages of host plant acceptance: host plant attraction, parturition (deposition of nymphs), nymph survival, and adult feeding behavior (using the electrical penetration graph [EPG]) to identify the location of the plant resistance. Aphids were more attracted to the susceptible cultivar 'Munger' (control) than the resistant selections. Parturition occurred on the resistant selections, but fewer nymphs were deposited on resistant lines relative to the susceptible control. Nymphs survived only an average of 3.3-3.6 days on resistant selections, whereas 94 % were still alive after 11 days on 'Munger.' There were differences in feeding behavior between the susceptible control and the resistant selections, but no differences between the three resistant selections. The tissues responsible for resistance appear to be the mesophyll and phloem sieve elements. Aphids had a reduced probability of salivation into the phloem sieve elements of resistant selections, and only one aphid each on ORUS 3778-1 and ORUS 4109-1 successfully ingested from the phloem. Because feeding behavior of A. agathonica did not differ between resistant selections, independent confirmation that resistance is conferred by unique genes should be obtained before pyramiding these sources together. C1 [Lightle, D.] Univ Calif Cooperat Extens, Orland, CA 95963 USA. [Dossett, M.] Agr & Agri Food Canada, British Columbia Blueberry Council, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Ebert, T.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Finn, C. E.; Martin, R. R.; Lee, J. C.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Lightle, D (reprint author), Univ Calif Cooperat Extens, POB 697, Orland, CA 95963 USA. EM dmlightle@ucanr.edu FU US Department of Agriculture Specialty Crops Research Initiative [2009-51181-06022]; CRIS [5358-22000-037-00D] FX Thanks to N. Mosier for propagation of the resistant black raspberries from tissue culture, North American Plants for donation of 'Munger' plants, B. Mackey for statistical advice, and C. Fieland and J. Mindolovich for assisting in behavioral assays. The authors declare no conflict of interest. Funding was provided by a US Department of Agriculture Specialty Crops Research Initiative grant 2009-51181-06022, and CRIS 5358-22000-037-00D. NR 41 TC 0 Z9 0 U1 1 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD OCT PY 2015 VL 9 IS 5 BP 487 EP 496 DI 10.1007/s11829-015-9390-z PG 10 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA CR1BB UT WOS:000361056600006 ER PT J AU Layman, ML Lundgren, JG AF Layman, Marissa L. Lundgren, Jonathan G. TI The influence of aphids (Myzus persicae) and pink lady beetle larvae (Coleomegilla maculata) on host plant preference of imported cabbageworm (Pieris rapae) SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Canola; Maternal preference; Multi-trophic interactions; Oviposition; Predator ID ENEMY-FREE SPACE; OVIPOSITION BEHAVIOR; CRUCIFEROUS VEGETABLES; PHYTOPHAGOUS INSECTS; CHEMICAL ECOLOGY; NATURAL ENEMIES; LEPIDOPTERA; SELECTION; BUTTERFLIES; SPECIALIZATION AB Oviposition decisions by herbivorous insects hinge on multiple factors, with some of the most important being enemy-free space and competition for resources. It is important to understand whether and how herbivores and predators can influence the maternal egg-laying preference when they are alone and in combination with host plants. Here, we evaluate whether the presence of aphids (a competitor) or a lady beetle larvae (a predator) influence host plant selection by an ovipositing butterfly. Canola (Brassica napus L.) was the highest quality of three putative Brassicaceae host plants for aphids Myzus persicae (Sulzer) (Hemiptera: Aphididae), while the butterfly Pieris rapae (L.) (Lepidoptera: Pieridae) showed similar survival on all. Canola was used to determine that the presence of a competitor herbivore (aphids) had no effect on butterfly oviposition behavior. However, predators significantly influenced the number of eggs laid on the plants, especially on those plants that had both aphids and a lady beetle larva present in combination. We expect that adult female P. rapae did not lay their eggs on the treatment that involved both herbivorous competition and predation risk, due to the combined risk factors along with the volatile chemicals and aphid alarm pheromones emitted on those plants that contained both the aphids and lady beetle larva. C1 [Layman, Marissa L.] S Dakota State Univ, Biol Microbiol Dept, Brookings, SD 57007 USA. [Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Lundgren, JG (reprint author), USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM jgl.entomology@gmail.com NR 60 TC 0 Z9 0 U1 5 U2 40 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD OCT PY 2015 VL 9 IS 5 BP 507 EP 514 DI 10.1007/s11829-015-9392-x PG 8 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA CR1BB UT WOS:000361056600008 ER PT J AU Lee, DH Leskey, TC AF Lee, D. -H. Leskey, T. C. TI Flight behavior of foraging and overwintering brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE invasive species; flight mill; dispersal; movement; pest management ID STAL HETEROPTERA; PERFORMANCE; DISPERSAL; ECOLOGY; PESTS; MOTH; MILL AB Brown marmorated stink bug, Halyomorpha halys (Stal), is a highly polyphagous invasive species attacking both cultivated and wild plants increasing its threat to ecosystems as a global pest. However, dispersal biology of this invasive species is not well understood. This study evaluated the flight capacity and behavior of H. halys under laboratory, semi-field, and field conditions. Flight mills were used to measure the baseline flight capacity of adults collected year round from the field and included both foraging and overwintering populations. The effects of abiotic conditions such as wind speed and temperatures on the free flight parameters of H. halys were evaluated under semi-field and field conditions. The mean flight distances over a 22-h period were 2442 and 2083 m for male and female, respectively. Most individuals (89%) flew <5 km, though some flew much further with a maximum flight distance observed of 117 km. Flight distances by H. halys increased after emergence from overwintering sites in spring and reached their highest point in June. The incidence of take off by H. halys was significantly affected by the wind speed; when provided with still air conditions, 83% of individuals took off, but the rates decreased to <10% when wind speed was increased to or above 0.75 m s(-1). The incidence of take off by H. halys was significantly affected by ambient temperature and light intensity in the field, whereas relative humidity and insect sex did not. When the temperature was at 10-15 degrees C, 3% of individuals took off, but the proportion of H. halys taking flight increased to 61, 84, and 87% at 15-20, 20-25, and 25-30 degrees C, respectively. In the field, prevailing flight direction was biased toward the opposite direction of the sun's position, especially in the morning. The implications of H. halys flight biology are discussed in the context of developing monitoring and management programs for this invasive species. C1 [Lee, D. -H.] Gachon Univ, Dept Life Sci, Songnam, Gyeonggi Do, South Korea. [Leskey, T. C.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. RP Lee, DH (reprint author), Gachon Univ, Dept Life Sci, 1342 Seongnamdaero, Songnam, Gyeonggi Do, South Korea. EM dl343@gachon.ac.kr FU Gachon University research fund [GCU-2014-0114]; USDA-NIFA SCRI [2011-51181-30937] FX We thank Cameron Scorza for excellent technical support. This work was supported in part by USDA-NIFA SCRI 2011-51181-30937 award. This research was also supported in part by the Gachon University research fund of 2014 (GCU-2014-0114). NR 33 TC 8 Z9 8 U1 7 U2 44 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD OCT PY 2015 VL 105 IS 5 BP 566 EP 573 DI 10.1017/S0007485315000462 PG 8 WC Entomology SC Entomology GA CR2HK UT WOS:000361148000005 PM 26074338 ER PT J AU Jennings, DE Duan, JJ Shrewsbury, PM AF Jennings, D. E. Duan, J. J. Shrewsbury, P. M. TI Biotic mortality factors affecting emerald ash borer (Agrilus planipennis) are highly dependent on life stage and host tree crown condition SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE EAB; forest insect; life tables; parasitoids; predation; tree resistance ID COLEOPTERA BUPRESTIDAE; NORTH-AMERICA; FRAXINUS SPP.; HYMENOPTERA ENCYRTIDAE; UNITED-STATES; IMPACTS; EULOPHIDAE; FAIRMAIRE; INVASION; BIOLOGY AB Emerald ash borer (EAB), Agrilus planipennis, is a serious invasive forest pest in North America responsible for killing tens to hundreds of millions of ash trees since it was accidentally introduced in the 1990s. Although host-plant resistance and natural enemies are known to be important sources of mortality for EAB in Asia, less is known about the importance of different sources of mortality at recently colonized sites in the invaded range of EAB, and how these relate to host tree crown condition. To further our understanding of EAB population dynamics, we used a large-scale field experiment and life-table analyses to quantify the fates of EAB larvae and the relative importance of different biotic mortality factors at 12 recently colonized sites in Maryland. We found that the fates of larvae were highly dependent on EAB life stage and host tree crown condition. In relatively healthy trees (i.e., with a low EAB infestation) and for early instars, host tree resistance was the most important mortality factor. Conversely, in more unhealthy trees (i.e., with a moderate to high EAB infestation) and for later instars, parasitism and predation were the major sources of mortality. Life-table analyses also indicated how the lack of sufficient levels of host tree resistance and natural enemies contribute to rapid population growth of EAB at recently colonized sites. Our findings provide further evidence of the mechanisms by which EAB has been able to successfully establish and spread in North America. C1 [Jennings, D. E.; Shrewsbury, P. M.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Duan, J. J.] ARS, USDA, Beneficial Insects Intro Unit, Newark, DE 19713 USA. RP Jennings, DE (reprint author), Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. EM david.e.jennings@gmail.com OI Jennings, David/0000-0001-7179-3069 FU USDA-ARS Specific Cooperative Agreement [58-1926-167]; USDA National Institute of Food and Agriculture, McIntire-Stennis Project [1003486] FX This work was supported by the USDA-ARS Specific Cooperative Agreement (58-1926-167), and the USDA National Institute of Food and Agriculture, McIntire-Stennis Project 1003486. We are extremely grateful to the Maryland Department of Agriculture for assistance with parasitoid releases and locating suitable study sites, particularly Dick Bean, Kim Rice, Charles Pickett, Steve Bell, Sam Stokes, Rose Buckner, Martin Proctor, and Aaron Shurtleff. We thank Mark Beals and Jesse Morgan (Green Ridge State Forest) for permission to use study sites, Jonathan Lelito and Stephanie Likens (USDA-APHIS) for providing parasitoids, and Kristi Larson, Jonathan Schmude, Sue Barth (all USDA-ARS), and Jackie Hoban (University of Delaware) for rearing EABs. We also thank Miles Wetherington, Trevor Scheffel, Meg Ryan, Jaclyn Mertz, Dan Nola, Katherine Rentas, Stokes Aker, Nancy Harding, Ashley Jones, and Grace Kunkel (all University of Maryland) for assistance in the field, and Doug Luster (USDA-ARS) and three anonymous reviewers for comments that greatly improved the manuscript. NR 52 TC 3 Z9 3 U1 5 U2 45 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD OCT PY 2015 VL 105 IS 5 BP 598 EP 606 DI 10.1017/S0007485315000498 PG 9 WC Entomology SC Entomology GA CR2HK UT WOS:000361148000009 PM 26072908 ER PT J AU Ryall, K Silk, P Webster, RP Gutowski, JM Meng, QF Li, Y Gao, WT Fidgen, J Kimoto, T Scarr, T Mastro, V Sweeney, JD AF Ryall, Krista Silk, Peter Webster, Reginald P. Gutowski, Jerzy M. Meng, Qingfan Li, Yan Gao, Wentao Fidgen, Jeff Kimoto, Troy Scarr, Taylor Mastro, Vic Sweeney, Jon D. TI Further evidence that monochamol is attractive to Monochamus (Coleoptera: Cerambycidae) species, with attraction synergised by host plant volatiles and bark beetle (Coleoptera: Curculionidae) pheromones SO CANADIAN ENTOMOLOGIST LA English DT Article ID TETROPIUM-FUSCUM F; SOUTHEASTERN UNITED-STATES; PINE SAWYER BEETLE; AGGREGATION PHEROMONE; GALLOPROVINCIALIS COLEOPTERA; SIMULTANEOUSLY ATTRACT; TITILLATOR COLEOPTERA; CINNAMOPTERUM KIRBY; LONGHORNED BEETLES; MULTIPLE-FUNNEL AB Monochamol (2-undecyloxy-1-ethanol) is a male-produced aggregation pheromone for several Monochamus Dejean (Coleoptera: Cerambycidae) species. We conducted trapping experiments in Canada, Poland, and China to test whether monochamol was attractive to additional Monochamus species and if attraction was synergised by plant volatiles and bark beetle (Coleoptera: Curculionidae) pheromones. We provide the first evidence of attraction for M. urussovii (Fischer) and M. saltuarius (Gebler) to monochamol or monochamol + kairomones. The highest numbers of M. urussovii were captured in traps baited with monochamol + plant volatiles (Manuka oil, ethanol and (95/5 +/-) alpha-pinene). Captures of M. saltuarius were highest in traps baited with monochamol, with the addition of cubeb oil tending to reduce captures. The highest numbers of M. scutellatus (Say) were captured in traps baited with monochamol + kairomones. A similar pattern in trap captures was found for M. notatus (Drury), M. marmorator Kirby, M. carolinensis (Olivier), and M. mutator LeConte. Detection rates, that is, proportion of traps capturing at least one specimen, was highest for traps baited with monochamol plus kairomones, particularly for less-common species. These results support the emerging hypothesis that pheromone compounds can attract related cerambycid species with cumulative evidence for attraction to monochamol for 12 species of Monochamus worldwide. C1 [Ryall, Krista; Fidgen, Jeff] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Silk, Peter; Webster, Reginald P.; Sweeney, Jon D.] Nat Resources Canada, Canadian Forest Serv, Atlantic Forestry Ctr, Fredericton, NB E3B 5P7, Canada. [Gutowski, Jerzy M.] European Ctr Nat Forests, Forest Res Inst, PL-17230 Bialowieza, Poland. [Meng, Qingfan] Beihua Univ, Coll Forestry, Fengman, Jilin, Peoples R China. [Li, Yan; Gao, Wentao] Beihua Univ, Fengman, Jilin, Peoples R China. [Kimoto, Troy] Canadian Food Inspect Agcy, Burnaby, BC V5C 6S7, Canada. [Scarr, Taylor] Ontario Minist Nat Resources, Sault Ste Marie, ON P6A 6B5, Canada. [Mastro, Vic] USDA, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. RP Ryall, K (reprint author), Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM kryall@nrcan.gc.ca FU Natural Resources Canada; Canadian Forest Service; United States Department of Agriculture Animal and Plant Health Inspection Service; Ontario Ministry of Natural Resources; Canadian Food Inspection Agency; Forest Protection Limited; SERG International FX This work was supported in part by Natural Resources Canada, Canadian Forest Service, United States Department of Agriculture Animal and Plant Health Inspection Service, Ontario Ministry of Natural Resources, Canadian Food Inspection Agency, Forest Protection Limited, and SERG International. The authors thank Katelyn Strong and Lin Tian Xi for permission to conduct experiments in the Acadia Research Forest, New Brunswick, and the Jiaohe Administration Bureau, Jilin, China, respectively. For technical assistance in the field and laboratory, the authors thank Chantelle Alderson, Chen Yibao, David Dutkievicz, Hugh Evans, Cory Hughes, Feng Li Chao, Isabelle Ochoa, Kathryn Nystrom, Lena van Seggelen, Krzysztof Sucko, Kalle Wainio, Vincent Webster, Wong Chong Shu, Li Lu Yao, Liu Yin, and Zhao Hong Rui. The authors thank Jeremy Allison and David Langor for constructive comments on an earlier draft of the manuscript. Finally, we thank Dave Davies for funding and support. NR 72 TC 11 Z9 11 U1 7 U2 27 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD OCT PY 2015 VL 147 IS 5 BP 564 EP 579 DI 10.4039/tce.2014.67 PG 16 WC Entomology SC Entomology GA CR0DI UT WOS:000360988100005 ER PT J AU Parks, LC Wallin, DO Cushman, SA Mcrae, BH AF Parks, Leslie C. Wallin, David O. Cushman, Samuel A. Mcrae, Brad H. TI Landscape-level analysis of mountain goat population connectivity in Washington and southern British Columbia SO CONSERVATION GENETICS LA English DT Article DE Population connectivity; GENELAND; Circuit theory; Causal modeling; Oreamnos americanus ID OREAMNOS-AMERICANUS POPULATION; GENE FLOW; CLIMATE-CHANGE; MANTEL TESTS; INBREEDING DEPRESSION; HABITAT FRAGMENTATION; COMPLEX LANDSCAPES; ALPINE UNGULATE; DIFFERENTIATION; RESISTANCE AB Habitat fragmentation and habitat loss diminish population connectivity, reducing genetic diversity and increasing extinction risk over time. Improving connectivity is widely recommended to preserve the long-term viability of populations, but this requires accurate knowledge of how landscapes influence connectivity. Detectability of landscape effects on gene flow is highly dependent on landscape context, and drawing conclusions from single landscape studies may lead to ineffective management strategies. We present a novel approach to elucidate regional variation in the relative importance of landscape variable effects on gene flow. We demonstrate this approach by evaluating gene flow between isolated, genetically impoverished mountain goat (Oreamnos americanus) populations in Washington and much larger, genetically robust populations in southern British Columbia. We used geneland to identify steep genetic gradients and then employed individual-based landscape genetics in a causal modeling framework to independently evaluate landscape variables that may be generating each of these genetic gradients. Our results support previous findings that freeways, highways, water, agriculture and urban landcover limit gene flow in this species. Additionally, we found that a previously unsupported landscape variable, distance to escape terrain, also limits gene flow in some contexts. By integrating geneland and individual-based methods we effectively identified regional limiting factors that have landscape-level implications for population viability. C1 [Parks, Leslie C.; Wallin, David O.] Western Washington Univ, Huxley Coll Environm, Dept Environm Sci, Bellingham, WA 98225 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Mcrae, Brad H.] North Amer Reg, Nat Conservancy, Ft Collins, CO 80524 USA. RP Wallin, DO (reprint author), Western Washington Univ, Huxley Coll Environm, Dept Environm Sci, 516 High St, Bellingham, WA 98225 USA. EM lesliecrparks@gmail.com; david.wallin@wwu.edu; scushman@fs.fed.us; bmcrae@tnc.org FU WDFW Aquatic Lands Enhancement Account; Seattle City Light; Mountaineers Foundation; Mazamas; Safari Club International Foundation; Washington Chapter of the Wildlife Society; Western Washington University Office of Research; Huxley College of the Environment FX We thank Andrew Shirk (University of Washington), Cliff Rice (WDFW), Brian Harris, Darryl Reynolds and Christ Proctor (BC Ministry of Forests, Lands and Natural Resource Operations), Cliff Nietvelt (BC Ministry of the Environment), Katy Chambers (BC Parks and Protected Areas), Aaron Shafer (Uppsala University), Kim Poole (Aurora Wildlife Research) and David Paetkau (Wildlife Genetics International) for their collaboration. We thank Samuel Wasser and Rebecca Nelson Booth (University of Washington) for assistance developing sample collection protocol and Ken Warheit, Scott Blankenship and Cheryl Dean (WDFW) for genotyping. Funding was provided by the WDFW Aquatic Lands Enhancement Account, Seattle City Light, the Mountaineers Foundation, the Mazamas, the Safari Club International Foundation, the Washington Chapter of the Wildlife Society, Western Washington University Office of Research and Sponsored Programs and Huxley College of the Environment. NR 79 TC 2 Z9 2 U1 17 U2 78 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD OCT PY 2015 VL 16 IS 5 BP 1195 EP 1207 DI 10.1007/s10592-015-0732-2 PG 13 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA CQ9MY UT WOS:000360940400014 ER PT J AU Iannone, BV Oswalt, CM Liebhold, AM Guo, QF Potter, KM Nunez-Mir, GC Oswalt, SN Pijanowski, BC Fei, SL AF Iannone, Basil V., III Oswalt, Christopher M. Liebhold, Andrew M. Guo, Qinfeng Potter, Kevin M. Nunez-Mir, Gabriela C. Oswalt, Sonja N. Pijanowski, Bryan C. Fei, Songlin TI Region-specific patterns and drivers of macroscale forest plant invasions SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE Biological invasions; Forest Inventory and Analysis Program; habitat invasibility; macrosystems; propagule pressure; spatial autocorrelation; spatial heterogeneity ID BIOLOGICAL INVASIONS; MACROSYSTEMS ECOLOGY; RHAMNUS-CATHARTICA; AREA RELATIONSHIPS; UNITED-STATES; IMPACTS; BIODIVERSITY; ECOSYSTEMS; REINVASION; FRAMEWORK AB AimStronger inferences about biological invasions may be obtained when accounting for multiple invasion measures and the spatial heterogeneity occurring across large geographic areas. We pursued this enquiry by utilizing a multimeasure, multiregional framework to investigate forest plant invasions at a subcontinental scale. LocationUnited States of America (USA). MethodsUsing empirical data from a national survey of USA forests, we compiled and mapped invasion richness (number of invasive species) and invasion prevalence (percentage of plots invaded) for 2524 counties. We then modelled each of these invasion measures as functions of 22 factors reflective of propagule pressure and/or habitat invasibility for eastern and western forests separately using simultaneous autoregressive spatial error models. ResultsEastern forests had higher mean invasion richness (6.1) and prevalence (48%) than western forests (3.2 and 10%, respectively). Spatial patterns of invasion richness and prevalence differed, especially in the West. Propagule pressure factors were always positively associated with both invasion measures. Factors associated with human legacy were nearly six times more strongly associated with western than eastern invasions. Unlike propagule pressure factors, habitat invasibility factors shared inconsistent associations with invasion measures. Main conclusionsThe weaker associations between human legacy and invasions in the heavily invaded East, compared to the less-invaded West, suggest a declining effect of propagule pressure over time with increasing invasion intensity. The importance of propagule pressure in less-invaded western forests suggests that spatial variability in propagule inputs, coupled with lags between establishment and commonness, drives the spatial differences between invasion richness and prevalence during early invasion stages. Meanwhile, declining spatial disagreement between invasion measures and the relative unimportance of propagule pressure, in heavily invaded eastern forests, suggest that species-specific variation in response to habitat invasibility drives spatial differences between invasion measures during later invasion stages. These insights further illustrate the importance of spatial heterogeneity in invasive plant management and policy at macroscales. C1 [Iannone, Basil V., III; Nunez-Mir, Gabriela C.; Pijanowski, Bryan C.; Fei, Songlin] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Oswalt, Christopher M.; Oswalt, Sonja N.] USDA, Forest Serv, Southern Res Stn, Knoxville, TN USA. [Liebhold, Andrew M.] USDA, Forest Serv, No Res Stn, Morgantown, WV USA. [Guo, Qinfeng] USDA, Forest Serv, Southern Res Stn, Asheville, NC USA. [Potter, Kevin M.] N Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC USA. RP Fei, SL (reprint author), Purdue Univ, Dept Forestry & Nat Resources, 715 W State St, W Lafayette, IN 47907 USA. EM sfei@purdue.edu OI Potter, Kevin/0000-0002-7330-5345; Fei, Songlin/0000-0003-2772-0166 FU NSF [1241932]; USDA Forest Service [13-JV-11330110-043]; North Carolina State University [13-JV-11330110-043] FX Thanks to the many FIA workers who collected the data used in this study; to Beth Schultz, Andrew Gray and Chris Witt for assisting with data compilation; and to Jarrod Doucette and Johanna Desprez for GIS-related advice. Comments from three anonymous referees improved this manuscript. This research was supported by NSF Macrosystems Biology grant # 1241932 and by a Research Joint Venture Agreement 13-JV-11330110-043 between the USDA Forest Service and North Carolina State University. NR 61 TC 5 Z9 5 U1 6 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 EI 1472-4642 J9 DIVERS DISTRIB JI Divers. Distrib. PD OCT PY 2015 VL 21 IS 10 BP 1181 EP 1192 DI 10.1111/ddi.12354 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR3BC UT WOS:000361204100005 ER PT J AU Zeilinger, AR Olson, DM Maclean, D Mori, N Nakata, R Andow, DA AF Zeilinger, Adam R. Olson, Dawn M. Maclean, Dan Mori, Naoki Nakata, Ryu Andow, David A. TI Behavioural and chemical mechanisms of plant-mediated deterrence and attraction among frugivorous insects SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Competition; consumer choice; facilitation; gossypol; induced resistance; induced susceptibility; Noctuidae; Pentatomidae ID STINK BUG HEMIPTERA; HOST-PLANT; INDUCED RESISTANCE; EUSCHISTUS-SERVUS; CEREAL APHIDS; PENTATOMIDAE; HERBIVORES; COMPETITION; PREFERENCE; RESPONSES AB 1. Herbivory often induces systemic plant responses that affect the host choice of subsequent herbivores, either deterring or attracting them, with implications for the performance of both herbivore and host plant. Combining measures of herbivore movement and consumption can efficiently provide insights into the induced plant responses that are most important for determining choice behaviour. 2. The preferences of two frugivorous stink bug species, Nezara viridula and Euschistus servus between cotton plants left undamaged or damaged by Helicoverpa zea and Heliothis virescens larvae were investigated. A novel consumer movement model was used to investigate if attraction rates or leaving rates determined preferences. Stink bug consumption rates were measured using salivary sheath flanges. Finally, the systemic induction of selected phenolic-based and terpenoid secondary metabolites were measured from heliothine herbivory on developing cotton bolls, to investigate if they explained stink bug feeding responses. 3. Heliothine herbivory did not affect the N. viridula feeding preference. However, we found opposing effects of H. zea and H. virescens herbivory on the behaviour of E. servus. Avoidance of H. zea-damaged plants is not obviously related to phenolic or terpenoid induction in cotton bolls; whereas a preference for H. virescens-damaged plants may be related to reductions in chlorogenic acid in boll carpel walls. 4. The present results highlight the inferential power of measuring both consumer movement and consumption in preference experiments and combining behavioural responses with phytochemical responses. Furthermore, while plant-mediated interactions among herbivorous insects are well studied, interactions among frugivorous species specifically have been poorly documented. C1 [Zeilinger, Adam R.; Andow, David A.] Univ Minnesota, Dept Entomol, Biol Program, St Paul, MN USA. [Olson, Dawn M.] USDA ARS, Crop Protect Res & Management Unit, Tifton, GA 31793 USA. [Maclean, Dan] Univ Georgia, Dept Hort, Tifton, GA USA. [Mori, Naoki; Nakata, Ryu] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto, Japan. [Andow, David A.] Univ Minnesota, Ctr Community Genet, St Paul, MN 55108 USA. RP Zeilinger, AR (reprint author), Univ Calif Berkeley, Berkeley Initiat Global Change Biol, 130 Mulford Hall 3114, Berkeley, CA 94720 USA. EM arz@berkeley.edu OI Zeilinger, Adam/0000-0002-4111-3568 FU National Research Initiative of US Department of Agriculture; National Institute of Food and Agriculture [2008-35302-04709]; US National Science Foundation IGERT program [0653827]; Thesis Research Grant; Doctoral Dissertation Fellowship; Dayton-Wilkie Fund of Bell Museum of Natural History; University of Minnesota FX This work was supported by the National Research Initiative of the US Department of Agriculture, National Institute of Food and Agriculture (grant number 2008-35302-04709 to DAA and DMO); the US National Science Foundation IGERT program (grant number 0653827 to the University of Minnesota); and a Thesis Research Grant, a Doctoral Dissertation Fellowship, and grants from the Dayton-Wilkie Fund of the Bell Museum of Natural History, University of Minnesota (to A.R.Z.). We would also like to thank A. Hornbuckle, M. Smith, A. Zittrouer, T. Brown, and J. Ransom for assistance in performing the experiments; C. Roberts, C. Blanco, and M. Toews for providing insects; T. Potter for assisting with terpenoid chemical analysis; P. Oikawa for illustrating our experimental design; and G. Heimpel, M. Daugherty, J. Thaler, D. Hare, two anonymous reviewers, and an associate editor for helpful comments on early drafts of this paper. We also thank the R Help on-line community for assistance with R programming. A. R. Zeilinger designed and conducted the preference experiment, with assistance from D. A. Andow and D. M. Olson. D. M. Olson and D. MacLean designed and conducted the chemical analysis of phenolic compounds. D. M. Olson, N. Mori, and R. Nakata designed and conducted the analysis of terpenoid analysis. A. R. Zeilinger and D. A. Andow designed the statistical analysis. A. R. Zeilinger wrote the manuscript with assistance from all co-authors. NR 55 TC 1 Z9 1 U1 1 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD OCT PY 2015 VL 40 IS 5 BP 532 EP 542 DI 10.1111/een.12221 PG 11 WC Entomology SC Entomology GA CR0KW UT WOS:000361008500006 ER PT J AU Bansal, S St Clair, JB Harrington, CA Gould, PJ AF Bansal, Sheel St Clair, J. Bradley Harrington, Constance A. Gould, Peter J. TI Impact of climate change on cold hardiness of Douglas-fir (Pseudotsugamenziesii): environmental and genetic considerations SO GLOBAL CHANGE BIOLOGY LA English DT Article DE assisted migration; climate transfer distance; cold acclimation; cold damage; conifer; frost; genecology; reciprocal transplant ID MENZIESII MIRB. FRANCO; FROST HARDINESS; FREEZING TOLERANCE; FOREST ECOSYSTEMS; DROUGHT TOLERANCE; WESTERN OREGON; PINUS-RADIATA; GROWTH; SEEDLINGS; TEMPERATURE AB The success of conifers over much of the world's terrestrial surface is largely attributable to their tolerance to cold stress (i.e., cold hardiness). Due to an increase in climate variability, climate change may reduce conifer cold hardiness, which in turn could impact ecosystem functioning and productivity in conifer-dominated forests. The expression of cold hardiness is a product of environmental cues (E), genetic differentiation (G), and their interaction (GxE), although few studies have considered all components together. To better understand and manage for the impacts of climate change on conifer cold hardiness, we conducted a common garden experiment replicated in three test environments (cool, moderate, and warm) using 35 populations of coast Douglas-fir (Pseudotsuga menziesii var.menziesii) to test the hypotheses: (i) cool-temperature cues in fall are necessary to trigger cold hardening, (ii) there is large genetic variation among populations in cold hardiness that can be predicted from seed-source climate variables, (iii) observed differences among populations in cold hardiness insitu are dependent on effective environmental cues, and (iv) movement of seed sources from warmer to cooler climates will increase risk to cold injury. During fall 2012, we visually assessed cold damage of bud, needle, and stem tissues following artificial freeze tests. Cool-temperature cues (e.g., degree hours below 2 degrees C) at the test sites were associated with cold hardening, which were minimal at the moderate test site owing to mild fall temperatures. Populations differed 3-fold in cold hardiness, with winter minimum temperatures and fall frost dates as strong seed-source climate predictors of cold hardiness, and with summer temperatures and aridity as secondary predictors. Seed-source movement resulted in only modest increases in cold damage. Our findings indicate that increased fall temperatures delay cold hardening, warmer/drier summers confer a degree of cold hardiness, and seed-source movement from warmer to cooler climates may be a viable option for adapting coniferous forest to future climate. C1 [Bansal, Sheel; Harrington, Constance A.; Gould, Peter J.] US Forest Serv, USDA, Pacific NW Res Stn, Olympia, WA 98512 USA. [St Clair, J. Bradley] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Gould, Peter J.] Washington State Dept Nat Resources, Olympia, WA 98504 USA. RP Bansal, S (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM sheelbansal9@gmail.com OI Bansal, Sheel/0000-0003-1233-1707 NR 75 TC 3 Z9 3 U1 7 U2 50 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD OCT PY 2015 VL 21 IS 10 BP 3814 EP 3826 DI 10.1111/gcb.12958 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR0FS UT WOS:000360994500021 PM 25920066 ER PT J AU Dilts, TE Weisberg, PJ Dencker, CM Chambers, JC AF Dilts, Thomas E. Weisberg, Peter J. Dencker, Camie M. Chambers, Jeanne C. TI Functionally relevant climate variables for arid lands: a climatic water deficit approach for modelling desert shrub distributions SO JOURNAL OF BIOGEOGRAPHY LA English DT Article DE Climatic water deficit; climograph; cold desert shrubs; gradient analysis; Great Basin; mechanistic model; PRISM; species distribution modelling; water balance; western USA ID SPECIES DISTRIBUTION MODELS; CONTERMINOUS UNITED-STATES; VEGETATION DISTRIBUTION; SPATIAL DEPENDENCE; GRADIENT ANALYSIS; BIG SAGEBRUSH; PATTERNS; BALANCE; RANGES; PREDICTION AB AimWe have three goals. (1) To develop a suite of functionally relevant climate variables for modelling vegetation distribution on arid and semi-arid landscapes of the Great Basin, USA. (2) To compare the predictive power of vegetation distribution models based on mechanistically proximate factors (water deficit variables) and factors that are more mechanistically removed from a plant's use of water (precipitation). (3) To quantify the climate gradients that control shrub distributions in a cold desert environment. LocationThe central basin and range ecoregion of the western USA (36-42 degrees N). MethodsWe used a modified Thornthwaite method to derive monthly water balance variables and to depict them using a water balance climograph. Eighteen variables were calculated from the climograph, representing different components of the seasonal water balance. These were used in boosted regression tree models to derive distribution models for 18 desert shrub species. The water balance approach was compared with an approach that used bioclimatic variables derived from the PRISM (Parameter-elevation Relationships on Independent Slopes Model) climate data. ResultsThe water balance and bioclimatic approaches yielded models with similar performance in predicting the geographical distribution of most shrub species. Cumulative annual climatic water deficit was consistently the most important water balance variable for predicting shrub type distributions, although predictions were improved by the inclusion of variables that describe the seasonal distribution of water balance such as water supply in the spring, fall actual evapotranspiration, monsoonality and summer decline in actual evapotranspiration. Main conclusionsThe water balance and bioclimatic approaches to species distribution modelling both yielded similar prediction accuracies. However, the water balance approach offers advantages over the bioclimatic approach because it is mechanistically derived to approximate physical processes important for plant growth. C1 [Dilts, Thomas E.; Weisberg, Peter J.; Dencker, Camie M.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Chambers, Jeanne C.] US Forest Serv, Rocky Mt Res Stn, Reno, NV 89512 USA. RP Dilts, TE (reprint author), 1664 N Virginia St, Reno, NV 89557 USA. EM tdilts@cabnr.unr.edu FU Joint fire Science Program Project [09-1-08-4] FX This research was funded largely by Joint fire Science Program Project #09-1-08-4. Patti Novak-Echenique from the USDA Natural Resource Conservation Service provided Ecological Site Description data which were used in an earlier version of the project. Dave Board provided helpful discussions throughout the project. Jian Yang, Ali Urza and Jerry Tiehm provided comments on earlier versions of this manuscript. We thank Joseph Veech, Janet Franklin and two anonymous referees for their reviews that greatly improved this manuscript. NR 46 TC 1 Z9 1 U1 4 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0305-0270 EI 1365-2699 J9 J BIOGEOGR JI J. Biogeogr. PD OCT PY 2015 VL 42 IS 10 BP 1986 EP 1997 DI 10.1111/jbi.12561 PG 12 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA CR2SX UT WOS:000361181900017 ER PT J AU Shen, QY Zhang, YZ Yang, RJ Hua, X Zhang, WB Zhao, W AF Shen, Qiuyun Zhang, Yuzhu Yang, Ruijin Hua, Xiao Zhang, Wenbin Zhao, Wei TI Thermostability enhancement of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus by site-directed mutagenesis SO JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC LA English DT Article DE Cellobiose 2-epimerase; Thermostatility; Site-directed mutagenesis; Semi-rational design ID SINGLE PROLINE SUBSTITUTION; PROTEIN THERMOSTABILITY; ASPERGILLUS-NIGER; FERULOYL ESTERASE; ALPHA-GLUCOSIDASE; STABILITY; DESIGN; LACTULOSE; RESIDUES; SEQUENCE AB Cellobiose 2-epimerase from the thermophile Caldicellulosiruptor saccharolydcus (CsCE) catalyzes the isomerization of lactose into lactulose, a non-digestible disaccharide widely used in food and pharmaceutical industries. Semi-rational approaches were applied to enhance the thermostability of CsCE. A total of eight single-site mutants were designed, and five of them showed prolonged half-life of inactivation at 80 degrees C. Combinatorial mutations were subsequently introduced, and the superior mutant was double mutant El 61D/N365P. The half-life was approximately 4-fold higher than that of the wild type enzyme. In addition, the reaction temperature for maximum activity increased from 80 degrees C to 87.5 degrees C, and catalytic efficiency (k(cat)/K-m) for lactulose production was increased 29%. Moreover, this mutant El 61D/N365P was more stable against chemical denaturation and showed also a broader pH profile. The second most stable variant were mutant E161D/S180P/S351G with a 3.3-fold increase in half-life. These results provided new insights into the thermostability of CsCE and suggested further potential industrial applications. (C) 2015 Elsevier B.V. All rights reserved. C1 [Shen, Qiuyun; Yang, Ruijin; Hua, Xiao; Zhang, Wenbin; Zhao, Wei] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China. [Shen, Qiuyun] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China. [Shen, Qiuyun; Zhang, Yuzhu] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Yang, RJ (reprint author), Jiangnan Univ, State Key Lab Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Peoples R China. EM yrj@jiangnan.edu.cn FU Key Project of National Science Fund of China [31230057]; National Key Technology R&D Program in the 12th Five Year Plan of China [2011BAD23B03]; Innovative Research Program for Graduate Students of Jiangsu Province [CXZZ11_0487] FX The authors gratefully thank Dominic Wong at WRRC, USDA-ARS for providing the instruments and reagents and giving valuable suggestions. The research was supported by the Key Project of National Science Fund of China (31230057), the National Key Technology R&D Program in the 12th Five Year Plan of China (2011BAD23B03), and the Innovative Research Program for Graduate Students of Jiangsu Province (CXZZ11_0487). NR 34 TC 1 Z9 1 U1 3 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1381-1177 EI 1873-3158 J9 J MOL CATAL B-ENZYM JI J. Mol. Catal. B-Enzym. PD OCT PY 2015 VL 120 BP 158 EP 164 DI 10.1016/j.molcatb.2015.07.007 PG 7 WC Biochemistry & Molecular Biology; Chemistry, Physical SC Biochemistry & Molecular Biology; Chemistry GA CR2MP UT WOS:000361164500020 ER PT J AU Mbuthia, LW Acosta-Martinez, V DeBruyn, J Schaeffer, S Tyler, D Odoi, E Mpheshea, M Walker, F Eash, N AF Mbuthia, Lilian Wanjiru Acosta-Martinez, Veronica DeBruyn, Jennifer Schaeffer, Sean Tyler, Donald Odoi, Evah Mpheshea, Molefi Walker, Forbes Eash, Neal TI Long term tillage, cover crop, and fertilization effects on microbial community structure, activity: Implications for soil quality SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Conservation agriculture; No-till; Till; Cover crops; Nitrogen fertilization; Soil quality; Microbial activity; Microbial biomass ID SOUTHERN HIGH-PLAINS; FATTY-ACID PROFILES; RESIDUE MANAGEMENT; ORGANIC-CARBON; CONSERVATION TILLAGE; NO-TILL; ENZYME-ACTIVITIES; REDUCED TILLAGE; NITROGEN; FUNGAL AB Conservation agriculture practices, such as reduced tillage, cover crops and fertilization, are often associated with greater microbial biomass and activity that are linked to improvements in soil quality. This study characterized the impact of long term (31 years) tillage (till and no-till), cover crops (Hairy vetch- Vicia villosa and winter wheat- Triticum aestivum, and a no cover control), and N-rates (0, 34, 67 and 101 kg N ha(-1)) on soil microbial community structure, activity and resultant soil quality calculated using the soil management assessment framework (SMAF) scoring index under continuous cotton (Gossypium hirsutum) production on a Lexington silt loam in West Tennessee. No-till treatments were characterized by a significantly greater (P < 0.05) abundance of Gram positive bacteria, actinomycetes and mycorrhizae fungi fatty acid methyl ester (FAME) biomarkers compared to till. Saprophytic fungal FAME biomarkers were significantly less abundant (P < 0,05) under no-till treatments resulting in a lower fungi to bacteria (F:B) ratio. Key enzymes associated with C, N & P cycling (beta-glucosidase, beta-glucosaminidase, and phosphodiesterase) had significantly higher rates under no-till relative to till, corresponding to significantly greater (P < 0.05) soil C and N, extractable nutrients (P, K and Ca) and yields. Mycorrhizae fungi biomarkers significantly decreased (P < 0.05) with increasing N-rate and was significantly less (P < 0.05) under the vetch cover crop compared to wheat and no cover. Treatments under vetch also had significantly higher beta-glucosaminidase and basal microbial respiration rates compared to wheat and no cover. Consequently, the total organic carbon (TOC) and beta-glucosidase SMAF quality scores were significantly greater under no-till compared to till and under the vetch compared to wheat and no cover treatments, resulting in a significantly greater overall soil quality index (SQI). Our results demonstrate that long-term no-till and use of cover crops under a low biomass monoculture crop production system like cotton results in significant shifts in the microbial community structure, activity, and conditions that favor C, N and P cycling compared to those under conventional tillage practices. These practices also led to increased yields and improved soil quality with no-till having 13% greater yields than till and treatments under vetch having 5% increase in soil quality compared to no cover and wheat. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Mbuthia, Lilian Wanjiru; DeBruyn, Jennifer; Schaeffer, Sean; Tyler, Donald; Odoi, Evah; Mpheshea, Molefi; Walker, Forbes; Eash, Neal] Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA. [Acosta-Martinez, Veronica] USDA ARS, Cropping Syst Res Lab, Wind Eros & Water Conservat Unit, Lubbock, TX 79401 USA. RP Mbuthia, LW (reprint author), Univ Tennessee, Dept Biosyst Engn & Soil Sci, 2506 EJ Chapman Dr, Knoxville, TN 37996 USA. EM lmbuthia@vols.utk.edu RI DeBruyn, Jennifer/A-8813-2010; Schaeffer, Sean/G-5071-2012; OI DeBruyn, Jennifer/0000-0002-2993-4144; Schaeffer, Sean/0000-0002-9684-2952; Eash, Neal/0000-0001-9141-4302 NR 69 TC 6 Z9 7 U1 37 U2 239 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD OCT PY 2015 VL 89 BP 24 EP 34 DI 10.1016/j.soilbio.2015.06.016 PG 11 WC Soil Science SC Agriculture GA CR2KZ UT WOS:000361159800003 ER PT J AU Yuan, J Chaparro, JM Manter, DK Zhang, RF Vivanco, JM Shen, QR AF Yuan, Jun Chaparro, Jacqueline M. Manter, Daniel K. Zhang, Ruifu Vivanco, Jorge M. Shen, Qirong TI Roots from distinct plant developmental stages are capable of rapidly selecting their own microbiome without the influence of environmental and soil edaphic factors SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Arabidopsis thaliana; Developmental stages; Microbiome; Phytohormone ID RHIZOSPHERE MICROBIOME; GROWTH; COMMUNITIES AB Soil microbes live in close association with plants and are crucial for plant health and fitness. Recent literature revealed that specific microbes were cultured at distinct developmental stages of Arabidopsis. It is not clear how fast the roots, depending on their developmental stage, can alter the root-associated microbiome. In this study, Arabidopsis, grown under sterile conditions at precisely distinct developmental stages were supplied with a soil microbial slurry. Within four days, roots selected specific microorganisms depending on plant development, and Proteobacteria among other bacterial groups were found to colonize the roots irrespective of developmental stage. Moreover, exposure to a microbiome resulted in modulation of phytohormone levels at different stages of Arabidopsis. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Yuan, Jun; Zhang, Ruifu; Shen, Qirong] Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Organ Solid Waste, Jiangsu Key Lab Organ Solid Waste Utilizat, Nanjing 210095, Jiangsu, Peoples R China. [Yuan, Jun; Chaparro, Jacqueline M.; Vivanco, Jorge M.] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. [Yuan, Jun; Chaparro, Jacqueline M.; Vivanco, Jorge M.] Colorado State Univ, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA. [Manter, Daniel K.] ARS, USDA, Soil Plant Nutrient Res Unit, Ft Collins, CO USA. RP Shen, QR (reprint author), Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Organ Solid Waste, Jiangsu Key Lab Organ Solid Waste Utilizat, Nanjing 210095, Jiangsu, Peoples R China. EM J.Vivanco@colostate.edu; shenqirong@njau.edu.cn RI Yuan, Jun/P-4658-2014 FU China Science and Technology Ministry (973 Program) [2015CB150500]; National Nature Science Foundation of China [31330069]; Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions; 111 project [B12009]; Colorado State University Agricultural Experiment Station; China Scholarship Council [201306850039] FX This study was financially supported by China Science and Technology Ministry (973 Program, 2015CB150500), National Nature Science Foundation of China (31330069), the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions, the 111 project (B12009), and by Colorado State University Agricultural Experiment Station. J. Y. was supported by China Scholarship Council (No. 201306850039). We also thank Charles Volmer for statistical consulting. NR 23 TC 4 Z9 4 U1 12 U2 89 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD OCT PY 2015 VL 89 BP 206 EP 209 DI 10.1016/j.soilbio.2015.07.009 PG 4 WC Soil Science SC Agriculture GA CR2KZ UT WOS:000361159800019 ER PT J AU Hinchliffe, D Condon, B Delhom, CD Chang, S Montalvo, J Madison, C Reynolds, M VonHoven, T Cintron, MS AF Hinchliffe, Doug Condon, Brian Delhom, Christopher D. Chang, SeChin Montalvo, Joseph Madison, Crista Reynolds, Michael VonHoven, Terri Cintron, Michael Santiago TI Physical and combustion properties of nonwoven fabrics produced from conventional and naturally colored cottons SO TEXTILE RESEARCH JOURNAL LA English DT Article DE calorimetry; cotton; flame retardant; hydroentanglement; needlepunch; nonwoven ID FIBER QUALITY; PIGMENTATION; LINT AB A comparative study was conducted to identify the effects of processing on physical and combustion properties of needlepunched (NP) and hydroentangled (H-E) nonwoven fabrics produced from fibers of white fiber cotton and a naturally colored brown fiber cotton. A significantly higher degree of flame retardancy (FR) in was observed in fabrics produced from brown cotton fibers compared with white fibers. Calorimetry revealed lower heat release capacity, lower peak heat release rate, and total heat release from brown fibers compared with white fibers. The ash content was also higher in brown fiber samples suggesting higher levels of inorganic elements in the brown fibers. Elemental analyses revealed brown cotton fibers had higher levels of known FR elements including phosphorous and magnesium. The H-E process reduced FR in brown fabrics, which also correlated with a reduction in phosphorous. However, brown H-E fabrics still maintained higher FR than white H-E fabrics. Water content analysis indicated higher water levels in brown fibers, particularly brown greige fibers, which correlated with increased FR. Processing parameters such as energy of H-E did not affect combustion of the two fabric types. Scouring of the brown fiber fabrics reduced, but did not remove coloration, while scouring and bleaching removed the brown color completely. Scouring alone, or scouring and bleaching, completely removed the higher FR properties of the brown fiber fabrics. The results indicate that the mechanism of FR in brown cotton fibers is dependent on multiple compositional factors that may include element content, water content, and compounds related to coloration. C1 [Hinchliffe, Doug; Condon, Brian; Delhom, Christopher D.; Chang, SeChin; Montalvo, Joseph; Madison, Crista; Reynolds, Michael; VonHoven, Terri; Cintron, Michael Santiago] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Hinchliffe, D (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM doug.hinchliffe@ars.usda.gov NR 40 TC 3 Z9 3 U1 5 U2 14 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 EI 1746-7748 J9 TEXT RES J JI Text. Res. J. PD OCT PY 2015 VL 85 IS 16 BP 1666 EP 1680 DI 10.1177/0040517515573410 PG 15 WC Materials Science, Textiles SC Materials Science GA CR2EQ UT WOS:000361138900002 ER PT J AU Greenberg, CH Goodrick, S Austin, JD Parresol, BR AF Greenberg, C. H. Goodrick, S. Austin, J. D. Parresol, B. R. TI Hydroregime Prediction Models for Ephemeral Groundwater-Driven Sinkhole Wetlands: a Planning Tool for Climate Change and Amphibian Conservation SO WETLANDS LA English DT Article DE Amphibian reproduction; Climate change; Ephemeral wetlands; Groundwater-driven wetlands; Hydroperiod; Hydroregime; Ocala national forest; Predictive models; Sinkhole wetlands ID EASTERN SPADEFOOT TOADS; FLORIDA LANDSCAPE; TEMPORAL ECOLOGY; UNITED-STATES; HYDROLOGY; HYDROPERIOD; PATTERNS; POOLS AB Hydroregimes of ephemeral wetlands affect reproductive success of many amphibian species and are sensitive to altered weather patterns associated with climate change. We used 17 years of weekly temperature, precipitation, and water-depth measurements for eight small, ephemeral, groundwater-driven sinkhole wetlands in Florida sandhills to develop a hydroregime predictive model. To illustrate its utility for climate-change planning, we forecasted weekly wetland water-depths and hydroperiods (2012-2060) using our model and downscaled climate data from the CSIRO Mk3.5 Global Circulation Model under an A1B emissions scenario. We then examined how forecasted water depths and hydroperiods might alter reproductive success, and thereby populations, of five anuran species. Precipitation and water-depth from the prior week were significant predictors of water depth. Our model forecasted shallower depths and shortened hydroperiods for most wetlands when used with the CSIRO Mk3.5 A1B scenario. The forecasted hydroregimes would likely provide adequate reproductive opportunity for only one of the five species we examined. We demonstrate the utility of our model in examining how different climate-change scenarios might affect hydroregimes and, indirectly, biological diversity. Climate change uncertainty highlights the importance of retaining multiple, hydrologically diverse wetlands on landscapes to maximize the potential for successful reproduction by species having differing hydroregime requirements. C1 [Greenberg, C. H.] USDA Forest Serv, Bent Creek Expt Forest, Southern Res Stn, Upland Hardwood Ecol & Management, Asheville, NC 28807 USA. [Goodrick, S.] USDA Forest Serv, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA 30602 USA. [Austin, J. D.] Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL 32611 USA. [Parresol, B. R.] USDA Forest Serv, Southern Res Stn, Asheville, NC USA. RP Greenberg, CH (reprint author), USDA Forest Serv, Bent Creek Expt Forest, Southern Res Stn, Upland Hardwood Ecol & Management, RWU 4157,1577 Brevard Rd, Asheville, NC 28807 USA. EM kgreenberg@fs.fed.us FU USDA Forest Service Ocala National Forest; Longleaf Pine Ecosystem Restoration Program; Southern Research Station; Southern Region [R8]; Department of Energy-Savannah River Operations (IA) [DE-AI09-76SR00056]; Florida Fish and Wildlife Conservation Commission [NG99-014, C1195] FX Funding was provided by the USDA Forest Service Ocala National Forest; Longleaf Pine Ecosystem Restoration Program; Southern Research Station; Southern Region (R8); Department of Energy-Savannah River Operations (IA Agreement DE-AI09-76SR00056); and the Florida Fish and Wildlife Conservation Commission (contracts NG99-014 and C1195). We thank J. Beach, S. Wazny, D. Wooten, R. Ashton, M. Welker, J. Smith, J. Staiger, J. Barichivich, R. Owen, D. Johnson, S. Johnson, J. Wiebe, K. Garren, E. Roznik, T. Sheltra, R. Ashton, S. Doucette-Riisse, C.J. Kovach, C. Bugbee, R. Lara, L. Tirado, and I. Luque for fieldwork. We also thank L. Lowery, R. Lowery, C. Sekerak, J. Hinchee, J. Clutts, J. Marr, M. Clere, M. Herrin, and the fire crew for assistance. We thank several anonymous reviewers and the Associate Editor for Wetlands for their helpful suggestions. NR 41 TC 1 Z9 1 U1 7 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD OCT PY 2015 VL 35 IS 5 BP 899 EP 911 DI 10.1007/s13157-015-0680-0 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CR0IQ UT WOS:000361002300006 ER PT J AU Evans, JJ Pasnik, DJ Klesius, PH AF Evans, Joyce J. Pasnik, David J. Klesius, Phillip H. TI Differential pathogenicity of five Streptococcus agalactiae isolates of diverse geographic origin in Nile tilapia (Oreochromis niloticus L.) SO AQUACULTURE RESEARCH LA English DT Article DE Streptococcus agalactiae; pathogenicity; bacteria; Oreochromis spp.; tilapia ID NONHEMOLYTIC GROUP-B; RED TILAPIA; FISH; INFECTION; INIAE; PCR; SUSCEPTIBILITY; VIRULENCE; DIFFICILE; VACCINE AB Streptococcus agalactiae is an emerging pathogen of fish and has caused significant morbidity and mortality worldwide. The main objective of this study is to assess whether pathogenic differences exist among isolates from different geographic locations. Nile tilapia (Oreochromis niloticus L.) were administered an intraperitoneal injection of suspension containing USA, Brazil, Honduras, Israel, or Kuwait S. agalactiae isolates at concentrations ranging from 10(2) to 10(7) cfu mL(-1). The LD50 values 7 days after challenge were as follows: USA (1.0 x 10(2) cfu mL(-1)), Brazil (1.5 x 10(3) cfu mL(-1)), Honduras (6.8 x 10(3) cfu mL(-1)), Israel (1.0 x 10(4) cfu mL(-1)) and Kuwait (7.2 x 10(5) cfu mL(-1)). Fish from all groups exhibited lethargy, anorexia, exophthalmia, corneal opacity, erratic swimming, petechiae and mortality. Opercular clearing and ascites were only found after infection with certain geographic isolates. The findings in this study indicate that S. agalactiae isolates of different geographic origin can cause significant mortalities after experimental challenge and can have different pathogenic capacities. Isolates from the Americas (USA, Brazil and Honduras) were more pathogenic to Nile tilapia than isolates from the Middle East/Asia (Israel and Kuwait). C1 [Evans, Joyce J.; Pasnik, David J.] Agr Res Serv, Aquat Anim Hlth Res Lab, USDA, Chestertown, MD USA. [Klesius, Phillip H.] Agr Res Serv, Aquat Anim Hlth Res Lab, USDA, Auburn, AL USA. RP Evans, JJ (reprint author), Agr Res Serv, Warm Water Aquaculture Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM joyce.evans@ars.usda.gov NR 37 TC 3 Z9 3 U1 0 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD OCT PY 2015 VL 46 IS 10 BP 2374 EP 2381 DI 10.1111/are.12393 PG 8 WC Fisheries SC Fisheries GA CQ5UR UT WOS:000360671700006 ER PT J AU Stewart, HA Bosworth, B Petrie-Hanson, L Martin, JA Allen, PJ AF Stewart, Heather A. Bosworth, Brian Petrie-Hanson, Lora Martin, James A. Allen, Peter J. TI Effects of chronic upper temperature regimes on growth of two geographical strains of channel and hybrid catfish SO AQUACULTURE RESEARCH LA English DT Article DE Channel catfish; hybrid catfish; chronic thermal stress; geographical; climate ID BLUE CATFISH; ICTALURUS-PUNCTATUS; EDWARDSIELLA-ICTALURI; WATER TEMPERATURE; FEED-UTILIZATION; TOLERANCE; ACCLIMATION; FISHERIES; SELECTION; SYSTEM AB Climate change is a growing concern for pond culture of catfish, due to possible exacerbation of temperature fluctuations and increased maximum daily temperatures. Because channel catfish (Ictalurus punctatus) have a broad natural distribution from Canada to Mexico, it was hypothesized that natural differences in thermal tolerance and seasonal growth may be attributed to different geographical strains. Furthermore, it was hypothesized that these differences would be observed in hybrid catfish (I. punctatus x [blue catfish] I. furcatus). Chronic thermal tolerance and growth rate were quantified in two geographically distinct strains of channel catfish and a corresponding hybrid catfish from one of these strains with a cross to an industry standard blue catfish strain. In a 6-week growth experiment, catfish were subjected to daily cycling temperatures of either 27-31 degrees C or 32-36 degrees C, mimicking pond fluctuations. Hybrid catfish had the highest survival at both temperatures, and both strains of channel catfish had greater growth in weight or length at 27-31 degrees C than at 32-36 degrees C. Therefore, these results indicate that physiological performance, in terms of growth, decreases in channel catfish at temperatures greater than 27-31 degrees C regardless of geographical origin of strain, whereas hybrid catfish did not show a decrease in weight under the same temperature conditions. C1 [Stewart, Heather A.; Martin, James A.; Allen, Peter J.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Bosworth, Brian] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. [Petrie-Hanson, Lora] Mississippi State Univ, Dept Basic Sci, Coll Vet Med, Mississippi State, MS 39762 USA. RP Allen, PJ (reprint author), Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, 9690, Mississippi State, MS 39762 USA. EM pallen@cfr.msstate.edu FU Natural Resource Enterprises; Mississippi Agricultural and Forestry Experimental Station; US Department of Agriculture (USDA) Agricultural Research Service; USDA National Institute of Food and Agriculture FX We thank the staff at the Thad Cochran National Warmwater Aquaculture Center for their assistance in spawning and rearing the fry used in this experiment; Dr Larry Hanson, Dr Wes Baumgartner and Dr Mark Lawrence at Mississippi State University College of Veterinary Medicine Fish Diagnostic Laboratory for their diagnostic work; students at Mississippi State University who helped transfer, weigh and measure fish; Mack Fondren and South Farm Aquaculture Facility staff for assistance. We also thank Natural Resource Enterprises, Mississippi Agricultural and Forestry Experimental Station, US Department of Agriculture (USDA) Agricultural Research Service, USDA National Institute of Food and Agriculture for funding and the two anonymous reviewers for providing helpful comments on this manuscript. NR 55 TC 0 Z9 0 U1 5 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD OCT PY 2015 VL 46 IS 10 BP 2407 EP 2417 DI 10.1111/are.12399 PG 11 WC Fisheries SC Fisheries GA CQ5UR UT WOS:000360671700010 ER PT J AU Skoracka, A Magalhaes, S Rector, BG Kuczynski, L AF Skoracka, Anna Magalhaes, Sara Rector, Brian G. Kuczynski, Lechosaw TI Cryptic speciation in the Acari: a function of species lifestyles or our ability to separate species? SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Review DE Biodiversity; Cryptic species; Hidden diversity; Integrative approach; Mites and ticks; Molecular systematics; Taxonomy ID WHEAT CURL MITE; CEREAL RUST MITE; BURYING BEETLES SILPHIDAE; ABACARUS-HYSTRIX ACARI; EXPERIMENTAL EVOLUTION; INTEGRATIVE TAXONOMY; CONFOCAL MICROSCOPY; HOST SPECIALIZATION; MULTIVARIATE DISCRIMINATION; REPRODUCTIVE COMPATIBILITY AB There are approximately 55,000 described Acari species, accounting for almost half of all known Arachnida species, but total estimated Acari diversity is reckoned to be far greater. One important source of currently hidden Acari diversity is cryptic speciation, which poses challenges to taxonomists documenting biodiversity assessment as well as to researchers in medicine and agriculture. In this review, we revisit the subject of biodiversity in the Acari and investigate what is currently known about cryptic species within this group. Based on a thorough literature search, we show that the probability of occurrence of cryptic species is mainly related to the number of attempts made to detect them. The use of, both, DNA tools and bioassays significantly increased the probability of cryptic species detection. We did not confirm the generally-accepted idea that species lifestyle (i.e. free-living vs. symbiotic) affects the number of cryptic species. To increase detection of cryptic lineages and to understand the processes leading to cryptic speciation in Acari, integrative approaches including multivariate morphometrics, molecular tools, crossing, ecological assays, intensive sampling, and experimental evolution are recommended. We conclude that there is a demonstrable need for future investigations focusing on potentially hidden mite and tick species and addressing evolutionary mechanisms behind cryptic speciation within Acari. C1 [Skoracka, Anna] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, Poznan, Poland. [Magalhaes, Sara] Univ Lisbon, Fac Sci, CE3c, P-1699 Lisbon, Portugal. [Rector, Brian G.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV USA. [Kuczynski, Lechosaw] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Avian Biol & Ecol, Poznan, Poland. RP Skoracka, A (reprint author), Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, Poznan, Poland. EM Anna.Skoracka@amu.edu.pl RI Kuczynski, Lechoslaw/E-5525-2011; Magalhaes, sara/B-9673-2012; OI Kuczynski, Lechoslaw/0000-0003-3498-5445; Magalhaes, sara/0000-0002-8609-7768; Skoracka, Anna/0000-0002-9485-532X FU National Science Centre in Poland (NSC) [DEC-2011/03/B/NZ8/00129]; NSC FX The authors would like to dedicate this work to the memory of Maurice Sabelis, who was an inspiration to acarologists around the world. This Project (conducted by AS and LK) was funded by the National Science Centre in Poland (NSC, Grant No. DEC-2011/03/B/NZ8/00129). The authors thank to Jacek Radwan (Evolutionary Biology Group, Adam Mickiewicz University [AMU]) for inspiration and fruitful discussion, Flore Zele for the discussion about symbiosis, Wiktoria Szydlo (AMU, Poznan, Poland) and Mariusz Lewandowski (SGGW, Warsaw, Poland) for participating in NSC Grant. NR 119 TC 4 Z9 4 U1 5 U2 35 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD OCT PY 2015 VL 67 IS 2 BP 165 EP 182 DI 10.1007/s10493-015-9954-8 PG 18 WC Entomology SC Entomology GA CQ6HG UT WOS:000360704600001 PM 26209969 ER PT J AU Machado-Ferreira, E Balsemao-Pires, E Dietrich, G Hojgaard, A Vizzoni, VF Scoles, G Bell-Sakyi, L Piesman, J Zeidner, NS Soares, CAG AF Machado-Ferreira, Erik Balsemao-Pires, Emilia Dietrich, Gabrielle Hojgaard, Andrias Vizzoni, Vinicius F. Scoles, Glen Bell-Sakyi, Lesley Piesman, Joseph Zeidner, Nordin S. Soares, Carlos A. G. TI Transgene expression in tick cells using Agrobacterium tumefaciens SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Transgene expression; Tick cells; Agrobacterium tumefaciens; Rhipicephalus microplus; Ixodes scapularis ID IXODES-SCAPULARIS TICKS; T-DNA; BORRELIA-BURGDORFERI; GENETIC-TRANSFORMATION; ANAPLASMA-MARGINALE; BOOPHILUS-MICROPLUS; HOST IMMUNITY; MODEL SYSTEM; PLANT-CELLS; INFECTION AB Ticks transmit infectious agents to humans and other animals. Genetic manipulation of vectors like ticks could enhance the development of alternative disease control strategies. Transgene expression using the phytopathogen Agrobacterium tumefaciens has been shown to promote the genetic modification of non-plant cells. In the present work we developed T-DNA constructs for A. tumefaciens to mediate transgene expression in HeLa cells as well as Rhipicephalus microplus tick cells. Translational fusions eGfp:eGfp or Salp15:eGfp, including the enhanced-green fluorescent protein and the Ixodes scapularis salivary factor SALP15 genes, were constructed using the CaMV 35S (cauliflower mosaic virus) promoter, "PBm" tick promoter (R. microplus pyrethroid metabolizing esterase gene) or the Simian Virus SV40 promoter. Confocal microscopy, RT-PCR and Western-blot assays demonstrated transgene(s) expression in both cell lines. Transgene expression was also achieved in vivo, in both R. microplus and I. scapularis larvae utilizing a soaking method including the A. tumefaciens donor cells and confirmed by nested-RT-PCR showing eGfp or Salp15 poly-A-mRNA(s). This strategy opens up a new avenue to express exogenous genes in ticks and represents a potential breakthrough for the study of tick-host pathophysiology. C1 [Machado-Ferreira, Erik; Balsemao-Pires, Emilia; Vizzoni, Vinicius F.; Soares, Carlos A. G.] Univ Fed Rio de Janeiro, Ilha Fundao, CCS, Inst Biol,Dept Genet,Lab Genet Mol Eucariontes &, BR-21941617 Rio De Janeiro, RJ, Brazil. [Machado-Ferreira, Erik; Dietrich, Gabrielle; Hojgaard, Andrias; Piesman, Joseph; Zeidner, Nordin S.] Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Bacterial Zoonoses Branch, Ft Collins, CO USA. [Scoles, Glen] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Bell-Sakyi, Lesley] Pirbright Inst, Surrey, England. RP Soares, CAG (reprint author), Univ Fed Rio de Janeiro, Ilha Fundao, CCS, Inst Biol,Dept Genet,Lab Genet Mol Eucariontes &, Bloco A,Sala A2-120, BR-21941617 Rio De Janeiro, RJ, Brazil. EM soares@biologia.ufrj.br RI Soares, Carlos/H-9464-2016; Institute, Pirbright/K-4476-2014 OI Soares, Carlos/0000-0001-9058-9266; FU CDC; Brazilian agency CAPES in the Departamento de Genetica-Instituto de Biologia (Universidade Federal do Rio de Janeiro); CAPES/Brazil; CNPq/Brazil FX Erik Machado-Ferreira's fellowship was supported by CDC and the Brazilian agency CAPES in the Departamento de Genetica-Instituto de Biologia (Universidade Federal do Rio de Janeiro). E. Balsemao-Pires was supported by a PhD fellowship from CAPES/Brazil and a SWE fellowship from CNPq/Brazil. The BME/CTVM2 cell line was provided by the Tick Cell Biobank. The anti-Salp15 antibody was supplied by Dr. Amy Ullmann-Moore of the CDC. NR 56 TC 2 Z9 2 U1 0 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD OCT PY 2015 VL 67 IS 2 BP 269 EP 287 DI 10.1007/s10493-015-9949-5 PG 19 WC Entomology SC Entomology GA CQ6HG UT WOS:000360704600007 PM 26188857 ER PT J AU Yoon, SC Lawrence, KC Park, B AF Yoon, Seung-Chul Lawrence, Kurt C. Park, Bosoon TI Automatic Counting and Classification of Bacterial Colonies Using Hyperspectral Imaging SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Hyperspectral imaging; Colony segmentation; Colony counting; Colony detection; Colony classification; Non-O157 STEC; Pathogen detection; Agar ID ESCHERICHIA-COLI SEROGROUPS; GROWTH-CHARACTERISTICS; MEASUREMENT UNCERTAINTY; UNITED-STATES; RAINBOW AGAR; SEPARATION; ALGORITHM; PATHOGENS; FUSARIUM; REGION AB Detection and counting of bacterial colonies on agar plates is a routine microbiology practice to get a rough estimate of the number of viable cells in a sample. There have been a variety of different automatic colony counting systems and software algorithms mainly based on color or gray-scale pictures although manual counting is still common. In microbiology, identification of presumptive-positive colonies on agar plates is predominantly done manually, which is laborious and time-consuming. This paper addresses a problem related to automatic colony segmentation and classification that can count the number of colonies according to their types. Hyperspectral imaging was used to develop a colony segmentation algorithm for detecting non-O517 Shiga-toxin producing Escherichia coli (STEC) pathogens on Rainbow agar. Hyperspectral absorbance image analysis in the visible and near-infrared spectral range from 400 to 1000 nm showed that colony morphology including size and texture was dependent on wavelength. The non-O157 STEC colonies showed dome-like absorbance profiles with local absorbance maxima. Touching colonies, causing problems for accurate counting and identification, were separated by optimally tessellating the mesh structure of local maximal points. The 428 nm was determined as the optimal wavelength for non-O157 STEC colony segmentation. The accuracy of the colony segmentation and counting algorithm was over 99 %. The average of the colony classification algorithm using automated colony segments was 92.5 %. C1 [Yoon, Seung-Chul; Lawrence, Kurt C.; Park, Bosoon] USDA ARS, Qual & Safety Res Unit, Athens, GA 30613 USA. RP Yoon, SC (reprint author), USDA ARS, Qual & Safety Res Unit, Athens, GA 30613 USA. EM seungchul.yoon@ars.usda.gov FU National Institute for Hometown Security FX This work was supported in part by a grant from The National Institute for Hometown Security. The authors would like to thank William R. Windham, Gerald W. Heitschmidt, Jerrie Barnett, and Peggy Feldner of USDA-ARS, Quality and Safety Assessment Research Unit, and Scott Ladely, Neelam Narang, and William C. Cray of the USDA-FSIS, Outbreaks Eastern Laboratory, in Athens, GA, USA for their assistance and help for this research. NR 53 TC 0 Z9 0 U1 5 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD OCT PY 2015 VL 8 IS 10 BP 2047 EP 2065 DI 10.1007/s11947-015-1555-3 PG 19 WC Food Science & Technology SC Food Science & Technology GA CQ4BQ UT WOS:000360549500005 ER PT J AU ul Haq, I Vreysen, MJB Caceres, C Shelly, TE Hendrichs, J AF ul Haq, Ihsan Vreysen, Marc J. B. Caceres, Carlos Shelly, Todd E. Hendrichs, Jorge TI Optimizing methyl-eugenol aromatherapy to maximize posttreatment effects to enhance mating competitiveness of male Bactrocera carambolae (Diptera: Tephritidae) SO INSECT SCIENCE LA English DT Article DE Bactrocera carambolae; mating competitiveness; ME aromatherapy; methyl-eugenol; ME feeding; posttreatment effect; SIT ID ORIENTAL FRUIT-FLY; MALE ANNIHILATION; STERILE MALES; DORSALIS DIPTERA; FLIES; ERADICATION; CONSUMPTION; SELECTION; BEHAVIOR; RELEASE AB Methyl-eugenol (ME) (1,2-dimethoxy-4-(2-propenyl)benzene), a natural phytochemical, did enhance male Bactrocera carambolae Drew & Hancock (Diptera: Tephritidae) mating competitiveness 3 d after ingestion. Enhanced male mating competitiveness can significantly increase the effectiveness of the sterile insect technique (SIT). ME application to mass reared sterile flies by feeding is infeasible. ME application by aromatherapy however, would be a very practical way of ME application in fly emergence and release facilities. This approach was shown to enhance mating competitiveness of B. carambolae 3 d posttreatment (DPT). Despite this added benefit, every additional day of delaying release will reduce sterile fly quality and will add cost to SIT application. The present study was planned to assess the effects of ME-aromatherapy on male B. carambolae mating competitiveness 1DPT and 2DPT. ME aromatherapy 1DPT or 2DPT did enhance mating competitiveness of B. carambolae males whereas ME feeding 1DPT and 2DPT did not. Male mating competitiveness was enhanced by the ME aromatherapy irrespective if they received 1DPT, 2DPT or 3DPT. ME aromatherapy, being a viable approach for its application, did enhance mating competitiveness of male B. carambolae 1d posttreatment as ME feeding did 3 d after ingestion. C1 [ul Haq, Ihsan; Vreysen, Marc J. B.; Caceres, Carlos] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Seibersdorf, Austria. [ul Haq, Ihsan] Natl Agr Res Ctr, Lahore, Pakistan. [Shelly, Todd E.] USDA, APHIS, Waimanalo, HI 96795 USA. [Hendrichs, Jorge] Joint FAO IAEA Div Nucl Tech Food & Agr Vienna, Insect Pest Control Sect, Vienna, Austria. RP ul Haq, I (reprint author), IAEA, Insect Pest Control Lab, Reaktorstr 1, A-1400 Vienna, Austria. EM I.Haq@iaea.org OI , Ihsan/0000-0001-5879-7698 NR 46 TC 0 Z9 0 U1 6 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD OCT PY 2015 VL 22 IS 5 BP 661 EP 669 DI 10.1111/1744-7917.12148 PG 9 WC Entomology SC Entomology GA CQ7EE UT WOS:000360764900008 ER PT J AU Dong, HT LaFrentz, B Pirarat, N Rodkhum, C AF Dong, H. T. LaFrentz, B. Pirarat, N. Rodkhum, C. TI Phenotypic characterization and genetic diversity of Flavobacterium columnare isolated from red tilapia, Oreochromis sp., in Thailand SO JOURNAL OF FISH DISEASES LA English DT Article DE 16S rRNA; 16S-23S rRNA intergenic spacer region; Flavobacterium columnare; genetic diversity; phenotypic characterization ID CATFISH ICTALURUS-PUNCTATUS; TROUT ONCORHYNCHUS-MYKISS; FISH PATHOGEN; FLEXIBACTER-COLUMNARIS; VIRULENCE; GENOMOVAR; STRAINS; DISEASE; WATER; ASSOCIATION AB Flavobacterium columnare is the aetiological agent of columnaris disease and severely affects various freshwater aquaculture fish species worldwide. The objectives of this study were to determine the phenotypic characteristics and genetic variability among F.columnare isolates isolated from red tilapia in Thailand. Forty-four F.columnare isolates were recovered from diseased fish in different geographical locations. The isolates exhibited homologous phenotypic characteristics but exhibited genetic diversity. One isolate was assigned to genomovar I, and the remainder were assigned to genomovar II, indicating the coexistence of these genomovars but predominance of genomovar II. Phylogenetic analysis of the 16S-23S ISR sequences revealed that a subset of the Thai isolates (n=25) contained a smaller intergenic spacer region (ISR) (523-537bp) and formed a unique ISR phylogenetic group. Phylogenetic analysis of the 16S rRNA gene supported the unique cluster of Thai isolates. This is the first description of the phenotypic and molecular characteristics of F.columnare isolated from red tilapia in Thailand as well as five isolates of F.columnare derived from other fish species including Nile tilapia, koi carp and striped catfish. C1 [Dong, H. T.; Rodkhum, C.] Chulalongkorn Univ, Fac Vet Sci, Dept Vet Microbiol, Bangkok 10330, Thailand. [LaFrentz, B.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL USA. [Pirarat, N.] Chulalongkorn Univ, Fac Vet Sci, Dept Vet Pathol, Bangkok 10330, Thailand. RP Rodkhum, C (reprint author), Chulalongkorn Univ, Fac Vet Sci, Dept Vet Microbiol, Bangkok 10330, Thailand. EM channarong.r@chula.ac.th RI Dong, Ha/N-4300-2016 OI Dong, Ha/0000-0002-7190-757X FU Chulalongkorn University; 90th anniversary of Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund) [GCUGR1125572134M] FX This research was supported by the graduate scholarship programme of Chulalongkorn University for faculty members from neighbouring countries and The 90th anniversary of Chulalongkorn University fund (Ratchadaphiseksomphot Endowment Fund) Grant No. GCUGR1125572134M. The authors would like to thank Dr. Dang Thi Hoang Oanh and Dr. Praparsiri Barnette for providing bacterial isolates (C31B, M1W, M1B, BU). We also thank staffs in the Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University for technical help. NR 38 TC 5 Z9 5 U1 0 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD OCT PY 2015 VL 38 IS 10 BP 901 EP 913 DI 10.1111/jfd.12304 PG 13 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA CQ7WC UT WOS:000360815500005 ER PT J AU Mateo-Sanchez, MC Balkenhol, N Cushman, S Perez, T Dominguez, A Saura, S AF Mateo-Sanchez, Maria C. Balkenhol, Niko Cushman, Samuel Perez, Trinidad Dominguez, Ana Saura, Santiago TI A comparative framework to infer landscape effects on population genetic structure: are habitat suitability models effective in explaining gene flow? SO LANDSCAPE ECOLOGY LA English DT Article DE Gene flow; Habitat suitability; Landscape resistance; Species movement; Landscape genetics; Brown bear ID BEAR URSUS-ARCTOS; CANTABRIAN BROWN BEAR; RESISTANCE SURFACES; NORTHERN SPAIN; MANTEL TESTS; CONNECTIVITY; CORRIDORS; SUBPOPULATIONS; PATCHINESS; SALAMANDER AB Most current methods to assess connectivity begin with landscape resistance maps. The prevailing resistance models are commonly based on expert opinion and, more recently, on a direct transformation of habitat suitability. However, habitat associations are not necessarily accurate indicators of dispersal, and thus may fail as a surrogate of resistance to movement. Genetic data can provide valuable insights in this respect. We aim at directly comparing the utility of habitat suitability models for estimating landscape resistance versus other approaches based on actual connectivity data. We develop a framework to compare landscape resistance models based on (1) a genetic-based multi model optimization and (2) a direct conversion of habitat suitability into landscape resistance. We applied this framework to the endangered brown bear in the Cantabrian Range (NW Spain). We found that the genetic-based optimization produced a resistance model that was more related to species movement than were models produced by direct conversion of habitat suitability. Certain land cover types and transport infrastructures were restrictive factors for species occurrence, but did not appear to impede the brown bear movements that determined observed genetic structure. In this study case, habitat suitability is not synonymous with permeability for dispersal, and does not seem to provide the best way to estimate actual landscape resistance. We highlight the general utility of this comparative approach to provide a comprehensive and practical assessment of factors involved in species movements, with the final aim of improving the initiatives to enhance landscape connectivity in conservation planning. C1 [Mateo-Sanchez, Maria C.; Saura, Santiago] Tech Univ Madrid, ETSI Montes Forestal & Medio Nat, Madrid 28040, Spain. [Balkenhol, Niko] Univ Gottingen, Dept Forest Zool & Forest Conservat, D-37077 Gottingen, Germany. [Cushman, Samuel] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Perez, Trinidad; Dominguez, Ana] Univ Oviedo, Dept Biol Func, E-33006 Oviedo, Spain. RP Mateo-Sanchez, MC (reprint author), Tech Univ Madrid, ETSI Montes Forestal & Medio Nat, Ciudad Univ S-N, Madrid 28040, Spain. EM mc.mateo@upm.es RI Dominguez, Ana/E-4412-2010; Saura, Santiago/A-9994-2008; OI Saura, Santiago/0000-0002-5239-0763; Dominguez-Sanjurjo, Ana/0000-0002-8610-5553 FU Spanish Ministry of Science and Innovation [AGL2012-31099]; Technical University of Madrid; government of "Principado de Asturias"; government of Junta de Castilla y Leon; Picos de Europa National Park; Fundacion Oso Pardo FX Funding was provided by the Spanish Ministry of Science and Innovation research grant GEFOUR (AGL2012-31099) and Technical University of Madrid. The non-invasive genotyping of bears was funded by the government of "Principado de Asturias", the government of Junta de Castilla y Leon and the Picos de Europa National Park along the years 2005 and 2010. We are also grateful to the Regional Administration involved in the brown bear management: Junta de Castilla y Leon, Gobierno de Cantabria, Principado de Asturias and Xunta de Galicia for providing data. Thanks also to the support provided by Fundacion Oso Pardo. NR 57 TC 10 Z9 10 U1 6 U2 62 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD OCT PY 2015 VL 30 IS 8 BP 1405 EP 1420 DI 10.1007/s10980-015-0194-4 PG 16 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CQ5VG UT WOS:000360673200004 ER PT J AU Kocyigit-Kaymakcioglu, B Beyhan, N Tabanca, N Ali, A Wedge, DE Duke, SO Bernier, UR Khan, IA AF Kocyigit-Kaymakcioglu, Bedia Beyhan, Nagihan Tabanca, Nurhayat Ali, Abbas Wedge, David E. Duke, Stephen O. Bernier, Ulrich R. Khan, Ikhlas A. TI Discovery and structure activity relationships of 2-pyrazolines derived from chalcones from a pest management perspective SO MEDICINAL CHEMISTRY RESEARCH LA English DT Article DE Chalcones; 2-Pyrazolines; Carbothioamide; Carboxamide; Aedes aegypti; Colletotrichum gloeosporioides; Botrytis cinerea; Lactuca sativa; Agrostis stolonifera; Lemna paucicostata ID ANTIINFLAMMATORY ACTIVITY; ANTIMICROBIAL EVALUATION; BIOLOGICAL EVALUATION; PYRAZOLE DERIVATIVES; ANTIMALARIAL AGENTS; MOSQUITO REPELLENTS; PLANT-PATHOGENS; ANTICANCER; FIPRONIL; CARBOXAMIDES AB Synthesis of chalcones and 2-pyrazoline derivatives has been an active field of research due to the established pharmacological effects of these compounds. In this study, a series of chalcone (1a-i), 2-pyrazoline-1-carbothioamides (2a-i), and 2-pyrazoline-1-carboxamide derivatives (3a-g) were synthesized and screened for their potential pesticide activities. The proposed structures of all the synthesized compounds were confirmed using the elemental analysis, UV, IR, H-1-NMR, and mass spectroscopy. Among the total of 25 tested compounds, compounds 1g and 2a and 2e with Biting Deterrence Index (BDI) values of 0.85, 0.83, and 0.8, respectively, at 25 nmol/cm(2) showed the highest biting deterrent activity against Aedes aegypti, which was comparable to N,N-diethyl-3-methylbenzamide (DEET). Compounds 1g, 2a and 2e were subsequently tested in human-based repellent bioassays, and they showed MED (minimum effective dose) values of 0.375, 0.094, and 0.375 mg/cm(2), respectively. Compound 1e was the most toxic compound (LC50 = 2.58 ppm), followed by 1f (LC50 = 5.69 ppm) and 2g (LC50 = 15.14 ppm), against 1-day-old Ae. aegypti larvae. Compounds 1f and 2h 1f and 2h showed the greatest growth inhibition against Colletotrichum gloeosporioides (97.6 and 98.5 %, respectively) at the lowest dose (0.3 mu M), which was greater antifungal activity than with standard commercial fungicides captan and azoxystrobin. Compounds 2d, 2g and 2h produced 79.5, 98.3, and 82.3 % growth inhibition, respectively, at 30.0 mu M against Botrytis cinerea, which was similar to captan in the antifungal activity. The active fungicidal compounds (2d, 2g, and 2h) were weaky phytotoxic, with little or no phytotoxicity at concentrations that were fungitoxic. Compound 2h stimulated the growth of Lemma paucicostata at concentrations that are fungitoxic to several plant pathogens. C1 [Kocyigit-Kaymakcioglu, Bedia; Beyhan, Nagihan] Marmara Univ, Fac Pharm, Dept Pharmaceut Chem, TR-34668 Istanbul, Turkey. [Tabanca, Nurhayat; Ali, Abbas; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Wedge, David E.; Duke, Stephen O.] Univ Mississippi, USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Bernier, Ulrich R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Khan, Ikhlas A.] Univ Mississippi, Dept BioMol Sci, Div Pharmacognosy, Sch Pharm, University, MS 38677 USA. RP Kocyigit-Kaymakcioglu, B (reprint author), Marmara Univ, Fac Pharm, Dept Pharmaceut Chem, TR-34668 Istanbul, Turkey. EM bkaymakcioglu@marmara.edu.tr FU Marmara University Scientific Research Projects Commission (BAPKO) [SAG-A-040609-0140]; USDA/ARS - US Department of Defense through the Armed Forces Pest Management Board [56-6402-1-612] FX This study was supported by Marmara University Scientific Research Projects Commission (BAPKO, Grant No. SAG-A-040609-0140) for the financial support and in part by USDA/ARS Grant No. 56-6402-1-612, Deployed War-Fighter Protection Research Program Grant funded by the US Department of Defense through the Armed Forces Pest Management Board. We thank Jessie Linda Robertson, Ramona Pace, Robert Johnson, Dr. Maia Tsikolia, Natasha M. Agramonte, Nathan Newlon, and Greg Allen for performing bioassays for this study and Dr. James J. Becnel, USDA-ARS, Gainesville, FL, for supplying mosquito eggs. NR 51 TC 4 Z9 4 U1 5 U2 19 PU SPRINGER BIRKHAUSER PI NEW YORK PA 233 SPRING STREET, 6TH FLOOR, NEW YORK, NY 10013 USA SN 1054-2523 EI 1554-8120 J9 MED CHEM RES JI Med. Chem. Res. PD OCT PY 2015 VL 24 IS 10 BP 3632 EP 3644 DI 10.1007/s00044-015-1415-8 PG 13 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA CQ3NF UT WOS:000360508300004 ER PT J AU Tintle, NL Newman, JW Shearer, GC AF Tintle, Nathan L. Newman, John W. Shearer, Gregory C. TI A novel approach to identify optimal metabotypes of elongase and desaturase activities in prevention of acute coronary syndrome SO METABOLOMICS LA English DT Article DE Likelihood ratio test; Metabotype estimation; Fatty acid activity indices ID C-REACTIVE PROTEIN; GLOBAL CARDIOVASCULAR RISK; FATTY-ACID DESATURATION; LIKELIHOOD RATIO TEST; ATHEROSCLEROSIS RISK; HEART-DISEASE; PREDICTION; COMMUNITIES; ELOVL6; METABOLOMICS AB Both metabolomic and genomic approaches are valuable for risk analysis, however typical approaches evaluating differences in means do not model the changes well. Gene polymorphisms that alter function would appear as distinct populations, or metabotypes, from the predominant one, in which case risk is revealed as changed mixing proportions between control and case samples. Here we validate a model accounting for mixed populations using biomarkers of fatty acid metabolism derived from a case/control study of acute coronary syndrome subjects in which both metabolomic and genomic approaches have been used previously. We first used simulated data to show improved power and sensitivity in the approach compared to classic approaches. We then used the metabolic biomarkers to test for evidence of distinct metabotypes and different proportions among cases and controls. In simulation, our model outperformed all other approaches including Mann-Whitney, t tests, and chi (2). Using real data, we found distinct metabotypes of six of the seven activities tested, and different mixing proportions in five of the six activity biomarkers: D9D, ELOVL6, ELOVL5, FADS1, and Sprecher pathway chain shortening (SCS). High activity metabotypes of non-essential fatty acids and SCS decreased odds for acute coronary syndrome, however high activity metabotypes of 20-carbon fatty acid synthesis increased odds. Our study validates an approach that accounts for both metabolomic and genomic theory by demonstrating improved sensitivity and specificity, better performance in real world data, and more straightforward interpretability. C1 [Tintle, Nathan L.] Dordt Coll, Dept Math Stat & Comp Sci, Sioux Ctr, IA USA. [Newman, John W.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Shearer, Gregory C.] Penn State Univ, Dept Nutr Sci, State Coll, PA 16801 USA. RP Shearer, GC (reprint author), Penn State Univ, Dept Nutr Sci, 110 Chandlee Lab, State Coll, PA 16801 USA. EM gcs13@psu.edu FU USDA [5306-51530-019-00D]; NIH [1U24DK097154-01, R15HG006915] FX The authors acknowledge John Spertus and William Harris for their work on the parent Project and in generating the data. Additional support was provided by the USDA Intramural Project 5306-51530-019-00D and the NIH, project 1U24DK097154-01 and R15HG006915. The USDA is an equal opportunity provider and employer. NR 41 TC 0 Z9 0 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD OCT PY 2015 VL 11 IS 5 BP 1327 EP 1337 DI 10.1007/s11306-015-0787-6 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CQ6LG UT WOS:000360715300027 ER PT J AU Sriwati, R Melnick, RL Muarif, R Strem, MD Samuels, GJ Bailey, BA AF Sriwati, Rina Melnick, Rachel L. Muarif, Rizky Strem, Mary D. Samuels, Gary J. Bailey, Bryan A. TI Trichoderma from Aceh Sumatra reduce Phytophthora lesions on pods and cacao seedlings SO BIOLOGICAL CONTROL LA English DT Article DE Cacao; Black pod rot; Trichoderma; Phytophthora ID BIOLOGICAL-CONTROL AGENTS; WITCHES-BROOM PATHOGEN; THEOBROMA-CACAO; BLACK-POD; SP NOV.; COCOA; DISEASES; MYCOPARASITISM; BIOCONTROL; CAMEROON AB The cocoa tree, Theobroma cacao L., suffers large yield losses in Aceh Indonesia due to the disease black pod rot, caused by Phytophthora spp. Despite having the largest area under cacao production in Sumatra, farmers in the Aceh region have low overall production because of losses to insect pests and black pod rot. Trichoderma spp. were isolated from the roots and leaves of cacao trees and screened as potential biological control agents. Isolates used in the study were Trichoderma asperellum isolates T2 and T4, Trichoderma longibrachiatum isolates T15 and T16, and Trichoderma virens isolates T1 and Tv. T1, T2, T4, and Tv completely colonized and destroyed Phytophthora tropicalis and Phytophthora palmivora mycelium in precolonized plate assays. All six isolates reduced P. tropicalis, but none reduced the growth of P. palmivora in dual plate assays. Phytophthora growth was suppressed on MIN media amended with sterile heat inactivated Trichoderma culture filtrates, with Tv best suppressing growth of both Phytophthora spp. T. virens isolate Tv was the only isolate observed coiling around P. tropicalis mycelium and disrupted the formation of P. palmivora sporangia. Of all six isolates, only Tv reduced P. palmivora lesion expansion in a detached pod assay, reducing severity by 71%. Tv also reduced P. palmivora infection on seedlings when applied aerially at 1 x 10(6) and 1 x 10(8) conidia/ml, by 19% and 59%, respectively. T. virens isolate Tv is a mycoparasite, antagonizes Phytophthora in a dual plate assay, and shows antibiosis against Phytophthora spp., suggesting that multiple modes of action contribute to its ability to limit Phytophthora lesion expansion on cacao pods and seedlings. Published by Elsevier Inc. C1 [Sriwati, Rina; Muarif, Rizky] Syiah Kuala Univ, Coll Agr, Agrotechnol Dept, Banda Aceh, Indonesia. [Melnick, Rachel L.; Strem, Mary D.; Bailey, Bryan A.] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD USA. [Samuels, Gary J.] Natl Inst Food & Agr, USDA, Washington, DC 20024 USA. [Samuels, Gary J.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. RP Melnick, RL (reprint author), Natl Inst Food & Agr, USDA, 800 9th St SW, Washington, DC 20024 USA. EM rachelmelnick@gmail.com FU World Cocoa Foundation; WCF-Aceh Cacao Fellowship Program; SwissContact's PEKA Project FX USDA is an equal opportunity provider and employer. The World Cocoa Foundation and the WCF-Aceh Cacao Fellowship Program under SwissContact's PEKA Project supported Dr. Swarti's travel and living expenses while at the USDA-ARS-SPCL. Fieldwork was supported by a Grant received from the SwissContact's PEKA Project. NR 49 TC 0 Z9 0 U1 2 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD OCT PY 2015 VL 89 BP 33 EP 41 DI 10.1016/j.biocontrol.2015.04.018 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CQ4BN UT WOS:000360549200004 ER PT J AU Duan, JJ Gould, JR Fuester, RW AF Duan, Jian J. Gould, Juli R. Fuester, Roger W. TI Evaluation of the host specificity of Spathius galinae (Hymenoptera: Braconidae), a larval parasitoid of the emerald ash borer (Coleoptera: Buprestidae) in Northeast Asia SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Host range; Risk assessment; Wood borers; Agrilus planipennis; Non-target ID TETRASTICHUS-PLANIPENNISI HYMENOPTERA; OOBIUS-AGRILI HYMENOPTERA; RUSSIAN FAR-EAST; BIOLOGICAL-CONTROL; EULOPHIDAE; ENCYRTIDAE; DORYCTINAE; FAIRMAIRE; ABUNDANCE; MICHIGAN AB Host-specificity determination prior to the introduction of non-native natural enemies is a critical component of the risk assessment for modern classical biocontrol programs. In the present study, we assessed the host specificity of a newly described parasitoid, Spathius galinae Belokobylskij and Strazanac, which is native to Northeast Asia, for classical biocontrol of the invasive emerald ash borer (EAB), Agrilus planipennis Fairmaire, in North America. Along with foreign exploration for natural enemies of EAB in 2010 and 2012, we surveyed ash (Fraxinus spp.) wood-boring insects and their parasitoids from artificially stressed Oriental ash (F. rhynchophylla Hance) trees in natural forest stands in the Primorskiy Kray region of Russia. Once colonies of S. galinae were established in U.S. quarantine, we tested the parasitoid against 15 species or groups of North American wood-boring insects that were selected along a continuum from closely related taxa (e.g., Agrilus spp. in the family Buprestidae in Coleoptera) to distantly related ones in Lepidoptera and Hymenoptera. While three of the tested insects, Neoclytus acuminatus Fabricius, Podosesia spp. and Hylesinus fraxini Panzer attack ash as their main host, the rest attack non-ash trees such as birch, maple and oak. Results from our field survey in Russia showed that S. galinae did not attack bark beetles (Hylesinus spp.) or longhorned beetles (Cerambycidae) that coexisted with EAB on infested ash trees. In quarantine laboratory studies, S. galinae did not attack 14 species or groups of non-target wood-boring insects infesting both ash and/or non-ash trees such as birch, maple or red oak. The only non-target attack was on the goldspotted oak borer (GSOB), Agrilus auroguttatus Schaeffer, infesting red oak. The rate of parasitism on GSOB was significantly lower than on EAB under laboratory conditions that favored parasitism. These findings indicate that the host specificity of S. galinae is restricted within the host genus (Agrilus). Published by Elsevier Inc. C1 [Duan, Jian J.; Fuester, Roger W.] USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Gould, Juli R.] USDA APHIS PPQ CPHST, Buzzards Bay, MA 02542 USA. RP Duan, JJ (reprint author), USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov NR 45 TC 3 Z9 3 U1 7 U2 39 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD OCT PY 2015 VL 89 BP 91 EP 97 DI 10.1016/j.biocontrol.2015.06.005 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CQ4BN UT WOS:000360549200011 ER PT J AU Talbot, NC Caperna, TJ AF Talbot, Neil C. Caperna, Thomas J. TI Proteome array identification of bioactive soluble proteins/peptides in Matrigel: relevance to stem cell responses SO CYTOTECHNOLOGY LA English DT Article DE Cell culture; Extracellular matrix; Matrigel; Protein array ID BASEMENT-MEMBRANE MATRIX; EXTRACELLULAR-MATRIX; GROWTH-FACTOR; NEURONAL DIFFERENTIATION; VASCULAR DEVELOPMENT; BIOLOGICAL-ACTIVITY; SELF-RENEWAL; CULTURE; SCAFFOLDS; SURVIVAL AB Matrigel and similar commercial products are extracts of the Engelbreth-Holm-Swarm sarcoma that provide a basement-membrane-like attachment substrate or gel that is used to grow cells on or in, respectively. To ascertain further what proteins may be present in Matrigel, besides its major basement-membrane constituents, an analysis of the expressed liquid of gelled Matrigel was performed using proteome array technology. Among the growth factors/cytokines assayed, high positive detection was found for IGFBP1, IGFBP3, LIF, platelet factor 4, PlGF-2, and VEGF; moderate reactivity was found for cyr61, IGFBP2, IGFBP6, IL-1ra, and NOV; and low, but detectable, responses occurred for aFGF, IL-13, IL-23, M-CSF, and VEGF-B. Among the chemokines assayed, high positive detection was found for MIG and serpin E1; moderate reactivity was found for IP-10, MCP-1, and MCP-5, and low, but detectable, responses occurred for CXCL16, I-TAC, and MIP-1 alpha. Among the other biologically active proteins assayed, high positive detection was found for adiponectin, C5a, endocan, lipocalin-2, sICAM-1, MMP-3, and TIMP-1; moderate reactivity was found for C-reactive protein, coagulation factor III, endoglin, endostatin/collagen XVIII, endothelin-1, ICAM-1, MMP-9, osteopontin, pentraxin-3, and RANTES; and low, but detectable, responses occurred for fetuin A, MMP-8, pentraxin-2, RBP4, resistin, and TIMP-4. The study found several growth factors, chemokines, and biologically active proteins not previously identified in Matrigel, and this may have significance to the interpretations of observed cellular responses when cells are grown on or in Matrigel. C1 [Talbot, Neil C.; Caperna, Thomas J.] ARS, Anim Biosci & Biotechnol Lab, BARC, USDA, Beltsville, MD 20705 USA. RP Talbot, NC (reprint author), ARS, Anim Biosci & Biotechnol Lab, BARC, USDA, East Bldg 200,Rm 13, Beltsville, MD 20705 USA. EM neil.talbot@ars.usda.gov NR 43 TC 1 Z9 1 U1 0 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-9069 EI 1573-0778 J9 CYTOTECHNOLOGY JI Cytotechnology PD OCT PY 2015 VL 67 IS 5 BP 873 EP 883 DI 10.1007/s10616-014-9727-y PG 11 WC Biotechnology & Applied Microbiology; Cell Biology SC Biotechnology & Applied Microbiology; Cell Biology GA CP9NR UT WOS:000360221700012 PM 24744128 ER PT J AU Adhikari, TB Mamidi, S Gurung, S Bonman, JM AF Adhikari, Tika B. Mamidi, Sujan Gurung, Suraj Bonman, John Michael TI Mapping of new quantitative trait loci (QTL) for resistance to Septoria tritici blotch in spring wheat (Triticum aestivum L.) SO EUPHYTICA LA English DT Article DE Genetics; Marker-assisted selection (MAS); Mycosphaerella graminicola; Resistance; Septoria tritici blotch; Triticum aestivum L ID STAGONOSPORA NODORUM BLOTCH; MYCOSPHAERELLA-GRAMINICOLA; HEXAPLOID WHEAT; CHROMOSOMAL LOCATION; GENETIC-ANALYSIS; TAN SPOT; CULTIVARS; IDENTIFICATION; DISEASES; ISOLATE AB Septoria tritici blotch (STB), caused by Zymoseptoria tritici (synonyms: Mycosphaerella graminicola and Septoria tritici), is a devastating disease of wheat (Triticum aestivum L.) worldwide. STB can be managed by fungicide application and by host resistance. The use of fungicides is less effective due to the development of fungal populations resistant to commercially available fungicides. As a result, host plant resistance is considered as one of the best strategies of STB management. In our preliminary study, the spring wheat cultivar 'Largo' showed a high level of resistant to multiple isolates of Z. tritici. The main objective of this study was to identify quantitative trait loci (QTL) for STB resistance in Largo and DNA markers associated with QTL. The 118 F-7 recombinant-inbred lines were developed from a cross between the synthetic hexaploid wheat Largo and the spring wheat susceptible line ND495 by single-seed descent. Disease severity in the mapping population was assessed at flag leaf stage 21 days after inoculation in the greenhouse at North Dakota State University, Fargo, ND in 2009 and 2010. Approximately, 2500 Diversity Arrays Technology(A (R)) (DArT) markers were used for genetic mapping. Among them, 263 DArT markers were polymorphic and revealed the 37 linkage groups. The new putative QTL (QStb.2A) associated with STB resistance was flanked by the two DArT markers wPt_3896 and wPt_4197, (wPt_4555) and is mapped on chromosome 2A. Single marker analysis also detected three additional DArT markers associated with other QTLs; however, chromosomal locations of these markers are unknown. Our results indicated that the DArT markers identified in this study can be useful for marker-assisted selection in wheat breeding programs and for combing this novel QTL with other STB resistance genes. C1 [Adhikari, Tika B.] N Carolina State Univ, Dept Plant Pathol, NSF Ctr Integrated Pest Management, Raleigh, NC 27606 USA. [Mamidi, Sujan] N Dakota State Univ, Genom & Bioinformat Program, NDSU Dept 7670, Fargo, ND 58108 USA. [Gurung, Suraj] Univ Calif Davis, Dept Plant Pathol, US Agr Res Stn, Salinas, CA 93905 USA. [Bonman, John Michael] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. RP Adhikari, TB (reprint author), N Carolina State Univ, Dept Plant Pathol, NSF Ctr Integrated Pest Management, Centennial Campus,840 Main Campus Dr, Raleigh, NC 27606 USA. EM tika_adhikari@ncsu.edu OI mamidi, sujan/0000-0002-3837-6121 FU North Dakota Wheat Commission; State Board of Agricultural Research and Education, North Dakota; USDA-ARS [58-5366-0-133] FX We gratefully acknowledge financial support from North Dakota Wheat Commission, and the State Board of Agricultural Research and Education, North Dakota, and USDA-ARS specific cooperative agreement 58-5366-0-133. We thank Steven Xu for providing the mapping population and Jana Hansen for technical help. NR 46 TC 2 Z9 2 U1 3 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD OCT PY 2015 VL 205 IS 3 BP 699 EP 706 DI 10.1007/s10681-015-1393-4 PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CQ1UD UT WOS:000360384300005 ER PT J AU Stommel, JR Whitaker, BD Haynes, KG Prohens, J AF Stommel, John R. Whitaker, Bruce D. Haynes, Kathleen G. Prohens, Jaime TI Genotype x environment interactions in eggplant for fruit phenolic acid content SO EUPHYTICA LA English DT Article DE Caffeoylquinic acid esters; Fruit quality; Heritability; Hydroxycinnamic acids; Phenolics; Solanum melongena ID CHLOROGENIC ACID; SOLANUM-MELONGENA; MASS SPECTROMETRY; CULTIVARS; POLYPHENOLS; COLLECTION; METABOLISM; DIVERSITY; CHEMISTRY; VARIETIES AB Eggplant fruit are a rich source of phenolic acids that influence fruit culinary quality and antioxidant content. We evaluated the influence of production environments and stability of diverse genotypes across environments for eggplant fruit phenolic acid content. Ten Solanum melongena accessions consisting of five F-1 hybrid cultivars, three open-pollinated cultivars and two land race accessions, plus one S. macrocarpon and one S. aethiopicum accession, were grown at two locations under greenhouse and open field environments. Twenty phenolic acid conjugates were identified in fruit flesh and assigned to six classes that included hydroxycinnamic acid amides, caffeoylquinic acid esters, hydroxycinnamoylquinic acid esters, malonylcaffeoylquinic acid esters, di-hydroxycinnamoylquinic acid esters, and other hydroxycinnamic acid conjugates. There were significant differences among accessions for total phenolic acid conjugate content and for all six classes. There were no significant differences detected among the environments for any of the variables. However, the environment x accession interaction was highly significant for all phenolic acid classes. Broad-sense heritability estimates for all six phenolic acid classes were high, ranging from 0.64 to 0.96. Stability analysis demonstrated widespread instability for phenolic acid content across environments. Stability of the predominant caffeoylquinic acid esters class positively influenced stability of total phenolic acid content for some but not all genotypes. High heritability, coupled with highly significant genotype x environment interactions suggests that stability estimates may improve the efficiency of breeding new genotypes with predictable performance across environments. C1 [Stommel, John R.; Haynes, Kathleen G.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville Agr Res Ctr,BARC West, Beltsville, MD 20705 USA. [Whitaker, Bruce D.] ARS, Food Qual Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Prohens, Jaime] Univ Politecn Valencia, Inst Conservac & Mejora Agrodiversidad Valenciana, Valencia 46022, Spain. RP Stommel, JR (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville Agr Res Ctr,BARC West, B-010A,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM john.stommel@ars.usda.gov RI Prohens, Jaime/H-2228-2015 OI Prohens, Jaime/0000-0003-1181-9065 NR 37 TC 3 Z9 3 U1 2 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD OCT PY 2015 VL 205 IS 3 BP 823 EP 836 DI 10.1007/s10681-015-1415-2 PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CQ1UD UT WOS:000360384300015 ER PT J AU Paris, HS Doron-Faigenboim, A Reddy, UK Donahoo, R Levi, A AF Paris, Harry S. Doron-Faigenboim, Adi Reddy, Umesh K. Donahoo, Ryan Levi, Amnon TI Genetic relationships in Cucurbita pepo (pumpkin, squash, gourd) as viewed with high frequency oligonucleotide-targeting active gene (HFO-TAG) markers SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Crop evolution; Cucurbita pepo subsp pepo; Cucurbitaceae; Nuclear DNA markers; Plant breeding; Pumpkin; Squash; Zucchini ID CULTIVAR-GROUPS; DOMESTICATION; DIVERSITY; EVOLUTION; AMERICA; IMAGES; AFLP; SRAP AB Cucurbita pepo is a highly diverse, economically important member of the Cucurbitaceae. C. pepo encompasses hundreds of cultivars of pumpkins, squash, and gourds. Although C. pepo has been scrutinized with various types of DNA markers, the relationships among the cultivar-groups of C. pepo subsp. pepo, the more widely grown subspecies, have not heretofore been adequately resolved. We assessed genetic relationships among 68 accessions of Cucurbita pepo, including 48 from C. pepo subsp. pepo, using polymorphisms in 539 high frequency oligonucleotide-targeting active gene (HFO-TAG) fragments, that preferably represent coding regions of the genome. Dissimilarities among accessions were calculated, a dendrogram was constructed, and principal component analyses were conducted. Dissimilarities demarcated the four edible-fruited cultivar-groups of C. pepo subsp. pepo, Cocozelle, Pumpkin, Vegetable Marrow, and Zucchini. Furthermore, the results indicate that the Old World pumpkins as well as the long-fruited cultivar-groups of C. pepo subsp. pepo (cocozelle, vegetable marrow, and zucchini) evolved from spontaneous crossing and gene exchange between pumpkins derived from northern North America and pumpkins derived from southern North America. Consistent with pictorial and narrative historical records, such crossing appears to have occurred in Renaissance Europe within the first decades of the European contact with North America. The Old World pumpkins are more closely related to the long-fruited cultivar-groups than are the native North American pumpkins. C1 [Paris, Harry S.] Agr Res Org, Newe Yaar Res Ctr, IL-30095 Ramat Yishay, Israel. [Doron-Faigenboim, Adi] Agr Res Org, Volcani Ctr, IL-50250 Bet Dagan, Israel. [Reddy, Umesh K.] West Virginia State Univ, Dept Biol, Institute, WV 25112 USA. [Donahoo, Ryan; Levi, Amnon] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. RP Paris, HS (reprint author), Agr Res Org, Newe Yaar Res Ctr, POB 1021, IL-30095 Ramat Yishay, Israel. EM hsparis@agri.gov.il NR 50 TC 4 Z9 4 U1 2 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD OCT PY 2015 VL 62 IS 7 BP 1095 EP 1111 DI 10.1007/s10722-015-0218-6 PG 17 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CQ1UQ UT WOS:000360385800013 ER PT J AU Patron, N Orzaez, D Marillonnet, S Warzecha, H Matthewman, C Youles, M Raitskin, O Leveau, A Farre, G Rogers, C Smith, A Hibberd, J Webb, AAR Locke, J Schornack, S Ajioka, J Baulcombe, DC Zipfel, C Kamoun, S Jones, JDG Kuhn, H Robatzek, S Van Esse, HP Sanders, D Oldroyd, G Martin, C Field, R O'Connor, S Fox, S Wulff, B Miller, B Breakspear, A Radhakrishnan, G Delaux, PM Loque, D Granell, A Tissier, A Shih, P Brutnell, TP Quick, WP Rischer, H Fraser, PD Aharoni, A Raines, C South, PF Ane, JM Hamberger, BR Langdale, J Stougaard, J Bouwmeester, H Udvardi, M Murray, JAH Ntoukakis, V Schafer, P Denby, K Edwards, KJ Osbourn, A Haseloff, J AF Patron, Nicola Orzaez, Diego Marillonnet, Sylvestre Warzecha, Heribert Matthewman, Colette Youles, Mark Raitskin, Oleg Leveau, Aymeric Farre, Gemma Rogers, Christian Smith, Alison Hibberd, Julian Webb, Alex A. R. Locke, James Schornack, Sebastian Ajioka, Jim Baulcombe, David C. Zipfel, Cyril Kamoun, Sophien Jones, Jonathan D. G. Kuhn, Hannah Robatzek, Silke Van Esse, H. Peter Sanders, Dale Oldroyd, Giles Martin, Cathie Field, Rob O'Connor, Sarah Fox, Samantha Wulff, Brande Miller, Ben Breakspear, Andy Radhakrishnan, Guru Delaux, Pierre-Marc Loque, Dominique Granell, Antonio Tissier, Alain Shih, Patrick Brutnell, Thomas P. Quick, W. Paul Rischer, Heiko Fraser, Paul D. Aharoni, Asaph Raines, Christine South, Paul F. Ane, Jean-Michel Hamberger, Bjoern R. Langdale, Jane Stougaard, Jens Bouwmeester, Harro Udvardi, Michael Murray, James A. H. Ntoukakis, Vardis Schaefer, Patrick Denby, Katherine Edwards, Keith J. Osbourn, Anne Haseloff, Jim TI Standards for plant synthetic biology: a common syntax for exchange of DNA parts SO NEW PHYTOLOGIST LA English DT Article DE cloning; DNA assembly; genetic syntax; GoldenGate; synthetic biology; Type IIS restriction endonucleases ID ONE-POT; CLONING; BIOTECHNOLOGY; TOOLS AB Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering. C1 [Patron, Nicola; Youles, Mark; Raitskin, Oleg; Zipfel, Cyril; Kamoun, Sophien; Jones, Jonathan D. G.; Kuhn, Hannah; Robatzek, Silke; Van Esse, H. Peter] Norwich Res Pk, Sainsbury Lab, Norwich NR4 7RG, Norfolk, England. [Patron, Nicola; Raitskin, Oleg; Smith, Alison; Hibberd, Julian; Webb, Alex A. R.; Locke, James; Schornack, Sebastian; Ajioka, Jim; Baulcombe, David C.; Sanders, Dale; Oldroyd, Giles; Martin, Cathie; Field, Rob; O'Connor, Sarah; Osbourn, Anne; Haseloff, Jim] Univ Cambridge, John Innes Ctr, OpenPlant Consortium, Norwich NR4 7UH, Norfolk, England. [Patron, Nicola; Matthewman, Colette; Raitskin, Oleg; Smith, Alison; Hibberd, Julian; Webb, Alex A. R.; Locke, James; Schornack, Sebastian; Ajioka, Jim; Baulcombe, David C.; Sanders, Dale; Oldroyd, Giles; Martin, Cathie; Field, Rob; O'Connor, Sarah; Osbourn, Anne; Haseloff, Jim] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England. [Orzaez, Diego; Granell, Antonio] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, E-46071 Valencia, Spain. [Marillonnet, Sylvestre; Tissier, Alain] Leibniz Inst Pflanzenbiochem, D-06120 Halle, Saale, Germany. [Warzecha, Heribert] Tech Univ Darmstadt, Plant Biotechnol & Metab Engn, D-64287 Darmstadt, Germany. [Matthewman, Colette; Leveau, Aymeric; Farre, Gemma; Rogers, Christian; Sanders, Dale; Oldroyd, Giles; Martin, Cathie; Field, Rob; O'Connor, Sarah; Fox, Samantha; Wulff, Brande; Miller, Ben; Breakspear, Andy; Radhakrishnan, Guru; Delaux, Pierre-Marc; Osbourn, Anne] Norwich Res Pk, John Innes Ctr, Norwich NR4 7UH, Norfolk, England. [Smith, Alison; Hibberd, Julian; Webb, Alex A. R.; Baulcombe, David C.; Haseloff, Jim] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England. [Locke, James; Schornack, Sebastian] Univ Cambridge, Sainsbury Lab, Cambridge CB2 1LR, England. [Ajioka, Jim] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England. [Loque, Dominique; Shih, Patrick] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA. [Loque, Dominique] Joint BioEnergy Inst, EmeryStn East, Emeryville, CA 94608 USA. [Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Quick, W. Paul] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England. [Rischer, Heiko] VTT Tech Res Ctr Finland, Espoo 02044, Finland. [Fraser, Paul D.] Univ London, Sch Biol Sci, Egham TW20 0EX, Surrey, England. [Aharoni, Asaph] Weizmann Inst Sci, Dept Plant Sci, IL-76100 Rehovot, Israel. [Raines, Christine] Univ Essex, Sch Biol Sci, Colchester CO4 3SQ, Essex, England. [South, Paul F.] USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Ane, Jean-Michel] Univ Wisconsin, Dept Bacteriol & Agron, Madison, WI 53706 USA. [Hamberger, Bjoern R.] Univ Copenhagen, Dept Plant & Environm Sci, Biochem Lab, Frederiksberg C, Denmark. [Langdale, Jane] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England. [Stougaard, Jens] Aarhus Univ, Dept Mol Biol & Genet, Ctr Carbohydrate Recognit & Signalling, Aarhus, Denmark. [Bouwmeester, Harro] Wageningen Univ, Wageningen UR, NL-6700 AA Wageningen, Netherlands. [Udvardi, Michael] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA. [Murray, James A. H.] Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales. [Ntoukakis, Vardis; Schaefer, Patrick; Denby, Katherine] Univ Warwick, Warwick Integrat Synthet Biol, Coventry CV4 7AL, W Midlands, England. [Ntoukakis, Vardis; Schaefer, Patrick; Denby, Katherine] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England. [Edwards, Keith J.] Univ Bristol, BrisSynBio, Bristol BS8 1TQ, Avon, England. RP Patron, N (reprint author), Norwich Res Pk, Sainsbury Lab, Norwich NR4 7RG, Norfolk, England. EM nicola.patron@tsl.ac.uk RI Granell, Antonio/G-3664-2010; ZIPFEL, CYRIL/D-7103-2011; Kuhn, Hannah/C-9984-2017; ORZAEZ, DIEGO/H-3457-2012; Loque, Dominique/A-8153-2008; Ane, Jean-Michel/G-5921-2010; Murray, James/D-3399-2009; Hamberger, Bjoern/I-2122-2012; Jones, Jonathan/J-5129-2012; Brutnell, Thomas/M-2840-2013; Kamoun, Sophien/B-3529-2009; Tissier, Alain/B-2108-2017; Wulff, Brande/C-7465-2013; OI ZIPFEL, CYRIL/0000-0003-4935-8583; Kuhn, Hannah/0000-0003-0145-1797; ORZAEZ, DIEGO/0000-0003-1662-5403; Ane, Jean-Michel/0000-0002-3128-9439; Murray, James/0000-0002-2282-3839; Hamberger, Bjoern/0000-0003-1249-1807; Jones, Jonathan/0000-0002-4953-261X; Brutnell, Thomas/0000-0002-3581-8211; Kamoun, Sophien/0000-0002-0290-0315; Tissier, Alain/0000-0002-9406-4245; Wulff, Brande/0000-0003-4044-4346; Baulcombe, David/0000-0003-0780-6878; Miller, Ben/0000-0003-0882-033X; Haseloff, Jim/0000-0003-4793-8058; van Esse, Peter/0000-0002-3667-060X; Field, Rob/0000-0001-8574-0275; Delaux, Pierre-Marc/0000-0002-6211-157X; Bouwmeester, Harro/0000-0003-0907-2732; Denby, Katherine/0000-0002-7857-6814; Radhakrishnan, Guru/0000-0003-0381-8804 FU Biotechnology and Biological Sciences Research Council [BBS/E/J/000C0618] NR 27 TC 21 Z9 21 U1 12 U2 76 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD OCT PY 2015 VL 208 IS 1 BP 13 EP 19 DI 10.1111/nph.13532 PG 7 WC Plant Sciences SC Plant Sciences GA CQ1RN UT WOS:000360376400005 PM 26171760 ER PT J AU Lawrence, SD Novak, NG AF Lawrence, Susan D. Novak, Nicole G. TI Mining the Brassica oleracea Genome for Q-type C2H2 Zinc Finger Transcription Factor Genes SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Q-type C2H2 zinc finger transcription factors; B. oleracea; Infestation; Biotic and abiotic stress; Phylogeny ID EXPRESSION ANALYSIS; PROTEINS; STRESS; TRIPLICATION; REPRESSORS; DROUGHT; MOTIF; COLD AB Q-type zinc finger proteins have been studied in several plant species and have been associated with response to stress. A whole genome analysis of Arabidopsis identified 176 putative C2H2 transcription factors (TF). Q-type C2H2 TFs containing the QALGGH motif and are a subset of these. In Arabidopsis, 18 have been described that contain two zinc finger motifs per protein, and several of these have been associated with a response to abiotic stress. The zinc fingers bind to the promoters of genes and negatively regulate transcription via the ethylene-responsive element-binding factor-associated amphiphilic repression (EAR) motif. Previously, we have identified StZFP2 from potato as induced upon infestation by insect pests. Interest in finding similar proteins in the newly annotated cabbage genome (Brassica oleracea var. capitata) led to the discovery of 25 proteins with 2-Q-type zinc finger domains. Examination of several transcriptome studies of B. oleracea submitted to the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) confirms the expression of all of these genes in at least one tissue type. Identification of the nearest homologs of these proteins in Arabidopsis and comparison of the map positions of both Arabidopsis and B. oleracea genes supports both the synteny between the genetic maps of these related species and the triplication event in Brassica species hypothesized to occur prior to the split from the ancestral progenitor of Arabidopsis and B. oleracea. C1 [Lawrence, Susan D.; Novak, Nicole G.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Lawrence, SD (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, 10300 Baltimore Ave BARC West,Bldg 007,Rm 301, Beltsville, MD 20705 USA. EM susan.lawrence@ars.usda.gov NR 20 TC 0 Z9 0 U1 6 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD OCT PY 2015 VL 33 IS 5 BP 1611 EP 1617 DI 10.1007/s11105-015-0857-y PG 7 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CQ1XE UT WOS:000360392800035 ER PT J AU Long, DS McCallum, JD AF Long, Dan S. McCallum, John D. TI On-combine, multi-sensor data collection for post-harvest assessment of environmental stress in wheat SO PRECISION AGRICULTURE LA English DT Article DE Precision agriculture; Multi-sensor fusion; Light detection and ranging; Harvest index ID GRAIN PROTEIN-CONCENTRATION; YIELD RESPONSE; HARVEST INDEX; SPRING WHEAT; NITROGEN; WATER; REFLECTANCE; FERTILIZER; SENSORS; DENSITY AB On-combine yield monitors are widely used in precision agriculture for locating areas within fields where yields are reduced. However, the crop yield variability may be better interpreted by utilizing grain protein maps to reveal the factors limiting yield. The objective of this study was to develop an on-combine multi-sensor system for obtaining site-specific measurements of grain yield, grain protein concentration, and straw yield at the same spatial resolution as grain yield. The methodology is based on a mass flow yield monitor, in-line near-infrared spectrometer, and light detection and ranging (LiDAR) instrument. The LiDAR sensor is used to indirectly estimate straw yield through the measurement of crop height. Neighborhoods within the individual grain yield and protein maps obtained by the yield monitor and the protein sensor are correlated to identify areas within fields where grain yield was limited by nitrogen stress or water stress. In addition, scatter plots of grain yield and straw yield, and deviations from the observed maximum slope, are used to identify specific regions of environmental stress. Multi-sensor data are acquired at coincident locations and thus, it is not necessary to interpolate data to a common estimation grid to enable their fusion. The on-combine, multi-sensor system is illustrated with results from farm fields in eastern Oregon, USA. C1 [Long, Dan S.; McCallum, John D.] USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. RP Long, DS (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, POB 370, Pendleton, OR 97801 USA. EM dan.long@oregonstate.edu NR 27 TC 1 Z9 1 U1 2 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD OCT PY 2015 VL 16 IS 5 BP 492 EP 504 DI 10.1007/s11119-015-9391-z PG 13 WC Agriculture, Multidisciplinary SC Agriculture GA CQ4IL UT WOS:000360568200002 ER PT J AU Thorp, KR Hunsaker, DJ French, AN Bautista, E Bronson, KF AF Thorp, K. R. Hunsaker, D. J. French, A. N. Bautista, E. Bronson, K. F. TI Integrating geospatial data and cropping system simulation within a geographic information system to analyze spatial seed cotton yield, water use, and irrigation requirements SO PRECISION AGRICULTURE LA English DT Article DE Crop model; Height; Inverse modeling; Leaf area index; Model inversion; Optimization; Precision agriculture; Remote sensing; Simulated annealing; Yield ID REMOTE-SENSING DATA; CERES-MAIZE MODEL; LEAF-AREA INDEX; MANAGEMENT STRATEGIES; PRECISION AGRICULTURE; SPRINKLER IRRIGATION; DECISION-SUPPORT; BALANCE MODELS; USE EFFICIENCY; LINT YIELD AB The development of sensors that provide geospatial information on crop and soil conditions has been a primary success for precision agriculture. However, further developments are needed to integrate geospatial data into computer algorithms that spatially optimize crop production while considering potential environmental impacts and resource limitations. The objective of this research was to combine several information technologies, including remote sensing, a cropping system model, and a geographic information system (GIS), to synthesize and interpret geospatial data collected during two irrigation scheduling experiments conducted in 2009 and 2011 in a 5-ha cotton field in central Arizona. The Geospatial Simulation (GeoSim) plug-in for Quantum GIS was used to manage geospatial data and conduct site-specific simulations with the CSM-CROPGRO-Cotton model. Simulated annealing optimization was used to adjust five model parameters to simulate site-specific conditions in 320 zones across the field. Using input parameters for GeoSim, a multiple criteria objective function was developed to incorporate measured and simulated leaf area index (LAI), crop canopy height, seed cotton yield, and evapotranspiration (ET) for site-specific optimization of CSM-CROPGRO-Cotton. Parameter identifiability and equifinality issues associated with model inversion were investigated. The optimized model was used for post hoc analysis of irrigation rates that maximized site-specific irrigation water use efficiency. With spatial optimization, the model was able to simulate LAI with root mean squared errors (RMSE) of 15 and 8 % in the 2009 and 2011 experiments, respectively. The RMSEs between measured and simulated canopy height, seed cotton yield, and ET were 5 % or less in both seasons. Some parameters were more identifiable than others during model inversions. Multiple temporal estimates of LAI were effective for constraining the model's specific leaf area parameter (SLAVR, cm(2) g(-1)), but lack of information on root growth reduce identifiability of a parameter related to that process (SRGF0). Post-hoc simulation analysis of irrigation management options showed that irrigation schedules based on remotely sensed vegetation indices increased irrigation water use efficiency as compared to traditional scheduling methods, particularly in the 2009 growing season. In 2011, the analysis showed that all scheduling methods resulted in excess irrigation application, and higher deep seepage rates were simulated in that season. Taken together, the results demonstrate that well-designed software tools and algorithms for data processing and interpretation can be potentially transformative for integrating multiple geospatial data sets to compute optimum scenarios for precision irrigation management. C1 [Thorp, K. R.; Hunsaker, D. J.; French, A. N.; Bautista, E.; Bronson, K. F.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Thorp, KR (reprint author), USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM kelly.thorp@ars.usda.gov RI Thorp, Kelly/C-2013-2009 OI Thorp, Kelly/0000-0001-9168-875X NR 58 TC 1 Z9 1 U1 8 U2 66 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD OCT PY 2015 VL 16 IS 5 BP 532 EP 557 DI 10.1007/s11119-015-9393-x PG 26 WC Agriculture, Multidisciplinary SC Agriculture GA CQ4IL UT WOS:000360568200004 ER PT J AU Liao, CH Zhang, HJ Wang, XP Li, DQ AF Liao, Chunhui Zhang, Houjiang Wang, Xiping Li, Dongqing TI Condition assessment of wood structural members at Yu Tomb of the Ming Dynasty, China SO MATERIALS AND STRUCTURES LA English DT Article DE Classification; Historic structure; Wood deterioration; Resistance micro-drilling; Stress wave timing; Renovation AB A detailed survey and inspection of the wood members at Yu Tomb of the Ming Dynasty in Beijing, China, was conducted during a structure renovation project. Visual classification and nondestructive inspection procedures were employed to assess the physical and mechanical conditions of the wood members taken down from the Soul Tower and the in-place members of the Three Gates and the Holy beam. Stress-wave-timing and resistance micro-drilling tests were found effective in detecting internal structural defects of the wood members in ancient structures and providing scientific data for accurate condition classification. The results of this inspection project provided the architects and construction engineers reliable data to develop a cost-effective renovation plan that can maximize the reuse of the original materials, at the same time ensure the safety of the historic structure. C1 [Liao, Chunhui; Zhang, Houjiang] Beijing Forestry Univ, Sch Technol, Beijing 100083, Peoples R China. [Wang, Xiping] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Li, Dongqing] Beijing Res Inst Architectural Heritages, Beijing, Peoples R China. RP Zhang, HJ (reprint author), Beijing Forestry Univ, Sch Technol, Beijing 100083, Peoples R China. EM cloudy_c119@163.com; hjzhang6@bjfu.edu.cn; xwang@fs.fed.us; lee5697@vip.sina.com FU Beijing Forestry University [11-MU-11111133-031]; USDA Forest Products Laboratory [11-MU-11111133-031]; Beijing Research Institute of Architectural Heritages FX This project was conducted through the research collaboration between Beijing Forestry University and the USDA Forest Products Laboratory (11-MU-11111133-031). The funding support from the Beijing Research Institute of Architectural Heritages is gratefully acknowledged. NR 14 TC 0 Z9 0 U1 1 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1359-5997 EI 1871-6873 J9 MATER STRUCT JI Mater. Struct. PD OCT PY 2015 VL 48 IS 10 BP 3259 EP 3267 DI 10.1617/s11527-014-0396-2 PG 9 WC Construction & Building Technology; Engineering, Civil; Materials Science, Multidisciplinary SC Construction & Building Technology; Engineering; Materials Science GA CP5RS UT WOS:000359942300014 ER PT J AU Yen, H Hoque, Y Harmel, RD Jeong, J AF Yen, Haw Hoque, Yamen Harmel, Robert Daren Jeong, Jaehak TI The impact of considering uncertainty in measured calibration/validation data during auto-calibration of hydrologic and water quality models SO STOCHASTIC ENVIRONMENTAL RESEARCH AND RISK ASSESSMENT LA English DT Article DE Measurement uncertainty; Model calibration; SWAT; Uncertainty analysis ID GOODNESS-OF-FIT; ASSESSMENT-TOOL; SWAT; SOIL AB The importance of uncertainty inherent in measured calibration/validation data is frequently stated in literature, but it is not often considered in calibrating and evaluating hydrologic and water quality models. This is due to the limited amount of data available to support relevant research and the limited scientific guidance on the impact of measurement uncertainty. In this study, the impact of considering measurement uncertainty during model auto-calibration was investigated in a case study example using previously published uncertainty estimates for streamflow, sediment, and NH4-N. The results indicated that inclusion of measurement uncertainty during the auto-calibration process does impact model calibration results and predictive uncertainty. The level of impact on model predictions followed the same pattern as measurement uncertainty: streamflow < sediment < NH4-N; however, the direction of that impact (increasing or decreasing) was not consistent. In addition, inclusion rate and spread results did not indicate a clear relationship between predictive uncertainty and the magnitude of measurement uncertainty. The purpose of this study was not to show that inclusion of measurement uncertainty produces better calibration results or parameter estimation. Rather, this study demonstrated that uncertainty in measured calibration/validation data can play a crucial role in parameter estimation during auto-calibration and that this important source of predictive uncertainty should be not be ignored as it is in typical model applications. Future modeling applications related to watershed management or scenario analysis should consider the potential impact of uncertainty in measured calibration/validation data, as model predictions influence decision-making, policy formulation, and regulatory action. C1 [Yen, Haw; Hoque, Yamen; Jeong, Jaehak] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. [Yen, Haw; Harmel, Robert Daren] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Yen, H (reprint author), Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM haw.yen@gmail.com FU United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components FX This study was supported by the funding from the United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components. Awesome comments provided by reviewers and editorial board greatly improved the quality of the manuscript. Also USDA is an equal opportunity employer and provider. NR 36 TC 2 Z9 2 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-3240 EI 1436-3259 J9 STOCH ENV RES RISK A JI Stoch. Environ. Res. Risk Assess. PD OCT PY 2015 VL 29 IS 7 BP 1891 EP 1901 DI 10.1007/s00477-015-1047-z PG 11 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences; Statistics & Probability; Water Resources SC Engineering; Environmental Sciences & Ecology; Mathematics; Water Resources GA CP3WW UT WOS:000359814500013 ER PT J AU Davidai, N Westbrook, JK Lessard, JP Hallam, TG McCracken, GF AF Davidai, Noa Westbrook, John K. Lessard, Jean-Philippe Hallam, Thomas G. McCracken, Gary F. TI The importance of natural habitats to Brazilian free-tailed bats in intensive agricultural landscapes in the Winter Garden region of Texas, United States SO BIOLOGICAL CONSERVATION LA English DT Article DE Conservation of threatened species; Land use change; Ecosystem services; Landscape management; Agricultural systems; Bats ID TADARIDA-BRASILIENSIS-MEXICANA; COMMUNITY STRUCTURE; CONVENTIONAL FARMS; SPECIES RICHNESS; LAND-USE; BIODIVERSITY; LEPIDOPTERA; CHIROPTERA; NOCTUIDAE; ABUNDANCE AB The conversion of natural lands to agriculture is a leading cause of the worldwide loss of biodiversity. In particular, cropland monocultures alter insect abundance and diversity compared to adjacent natural habitats. While agricultural lands can provide large numbers of insect pests as prey items to predators such as bats, insect pest population size vary greatly throughout growing seasons. This study assesses the importance of land use and corn earworm moth availability as spatial and temporal drivers of bat activity. We quantified spatial variation in land use patterns at fifteen sites located within the Winter Garden region in south central Texas and used bat detectors and insect pheromone traps to monitor nightly bat activity and corn earworm moth abundance across the landscape and throughout most of the year. Our temporal analyses show that bat activity was positively correlated with moth abundance, but only early in the growing season when moth abundance is at its peak. The key result from this study is a positive relationship between bat activity and natural habitat cover during late summer months, corresponding to periods of low moth abundance and a peak in bat activity. During the late summer period, bats were more active at sites containing a larger percentage of natural habitats than those containing a larger percentage of agricultural land. Our results strongly suggest that intensive agricultural practices create systems providing bats with inconsistent resource supply, but the persistence of natural habitats provides consistency in food supply though time. Taken together, these findings illustrate the importance of protecting and restoring natural habitats for the conservation for bats and the pest-suppression services they provide in agricultural ecosystems. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Davidai, Noa; Lessard, Jean-Philippe; Hallam, Thomas G.; McCracken, Gary F.] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA. [Westbrook, John K.] USDA ARS, Insect Control & Cotton Dis Res Unit, College Stn, TX 77845 USA. [Lessard, Jean-Philippe] Concordia Univ, Dept Biol, Montreal, PQ H4B 1R6, Canada. RP Davidai, N (reprint author), 7213 Somerled Ave, Montreal, PQ H4V 1W4, Canada. EM ndavidai@hotmail.com; john.westbrook@ars.usda.gov; jp.lessard@concordia.ca; thallam@utk.edu; gmccrack@utk.edu OI McCracken, Gary/0000-0002-2493-8103 FU Department of Ecology and Evolutionary Biology at the University of Tennessee, a Bat Conservation International Student Research Scholarship; NSF [EIA-0326483] FX We thank Mariano Rodriguez-Cabal and Aaron Gove for providing comments on early draft of the manuscript. Rodney Sams, Seth Walker and Thomas Maslen provided valuable assistance with the fieldwork. We thank three anonymous reviewers for their comments, which significantly improved previous versions of the manuscript. We also thank the numerous agricultural landowners who provided us access to field sites. Noa Davidai was supported by the Department of Ecology and Evolutionary Biology at the University of Tennessee, a Bat Conservation International Student Research Scholarship, and NSF Grant EIA-0326483 (TH Kunz, PI, M Betke, GF McCracken, P Morton, and JK Westbrook, CoPI's). NR 54 TC 2 Z9 2 U1 14 U2 92 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD OCT PY 2015 VL 190 BP 107 EP 114 DI 10.1016/j.biocon.2015.05.015 PG 8 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CO2FS UT WOS:000358972200013 ER PT J AU Linz, GM Bucher, EH Canavelli, SB Rodriguez, E Avery, ML AF Linz, George M. Bucher, Enrique H. Canavelli, Sonia B. Rodriguez, Ethel Avery, Michael L. TI Limitations of population suppression for protecting crops from bird depredation: A review SO CROP PROTECTION LA English DT Review DE Avian pests; Blackbirds; Crop damage; Dickcissel; Eared dove; Granivorous birds ID RED-WINGED BLACKBIRDS; MONK PARAKEETS; MANAGEMENT STRATEGIES; AGELAIUS-PHOENICEUS; SOUTH-DAKOTA; LAND-USE; DAMAGE; DICKCISSELS; ARGENTINA; VENEZUELA AB Blackbirds (Icterinae) in North America, and dickcissels (Spiza americana Gmelin), eared doves (Zenaida auriculata Des Murs), and monk parakeets (Myiopsitta monachus Boddaert) in South America can cause serious economic damage to grain crops. Farmers frequently advocate lethal bird damage abatement measures based on the perceived need to take immediate action to avoid serious economic losses. In comparison, wildlife managers must make informed decisions based on a multitude of factors, including local, state, and national environmental laws, administrative restrictions, logistics, costs, expected outcome, and cultural considerations related to wildlife stewardship. In this paper, we focus on practicality, environmental safety, cost-effectiveness and wildlife stewardship to evaluate efforts to manage avian crop damage using lethal control. In each case where a lethal program was initiated, at least one of these four tenets was violated and there was temporary relief at best. Published by Elsevier Ltd. C1 [Linz, George M.] USDA WS, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. [Bucher, Enrique H.] Univ Nacl Cordoba, Ctr Zool Aplicada, Inst Diversidad & Ecol Anim CONICET UNC, RA-5000 Cordoba, Argentina. [Bucher, Enrique H.] Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, RA-5000 Cordoba, Argentina. [Canavelli, Sonia B.] Natl Inst Agr Technol, Parana Agr Expt Stn, Oro Verde, Entre Rios, Argentina. [Rodriguez, Ethel] INIA, Vertebrate Pest Management Dept, Montevideo 4703, Uruguay. [Rodriguez, Ethel] MGAP, Montevideo 4703, Uruguay. [Avery, Michael L.] USDA WS, Natl Wildlife Res Ctr, Gainesville, FL USA. RP Linz, GM (reprint author), USDA WS, 2110 Miriam Circle,Suite B, Bismarck, ND 58501 USA. EM george.m.linz@aphis.usda.gov; buchereh@gmail.com; canavelli.sonia@inta.gob.ar; ethel.rodriguez@gmail.com; michaellavery@aphis.usda.gov NR 63 TC 0 Z9 1 U1 8 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD OCT PY 2015 VL 76 BP 46 EP 52 DI 10.1016/j.cropro.2015.06.005 PG 7 WC Agronomy SC Agriculture GA CO7GF UT WOS:000359326900007 ER PT J AU Sun, X Yan, MJ Zhang, AJ Wang, MQ AF Sun, Xiao Yan, Miao-Jun Zhang, Aijun Wang, Man-Qun TI Transgenic cry1C* gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Bacillus thuringiensis; Rough rice; Volatile chemicals; Physical character; Non-target effect; Tritrophic bioassay ID BACILLUS-THURINGIENSIS MAIZE; ELECTRONIC NOSE; PLODIA-INTERPUNCTELLA; PLANT VOLATILES; INDICA RICE; MILLED RICE; BT COTTON; INSECT; RESPONSES; TONGUE AB Rough rice grains are often stored for extended periods before they are used or consumed. However, during storage, the rough rice is vulnerable to insect infestation, resulting in significant economic loss. Previous studies have shown that volatiles cues, physical characteristics, and taste chemicals on the grains could be the important key behavior factors for storage insect pests to locate the hosts and select oviposition sites. It is also well known that the transgenic Bt rough rice line TIC-19, which expresses a cry1C* gene has a high resistance to Lepidoptera pests. However, there were no evidences to show the consequences of host preference for non-target insect pests after growing Bt transgenic rice. In this study, the potential key factors of Bt rough rice were investigated for their impacts on the behaviors of nontarget pest lesser grain borer Rhyzopertha dominica, the main weevil pest of grain and its parasitic wasps Anisopteromalus calandrae, the natural enemy of the beetle. Both electronic nose and electronic tongue analyses showed that the parameters of Bt rough rice were analogous to those of the non-Bt rough rice. The volatile profiles of Bt and non-Bt rough rice examined by gas chromatographic mass spectrometry (GC-MS) were similar. For most volatile compounds, there were no significantly quantitative differences in compound quantities between Bt and non-Bt rough rice. The densities of sclereids and trichomes on the rough rice husk surface were statistically equal in Bt and non-Bt rough rice. The non-target pest, R. dominica, and its parasitoid wasp, A. calandrae, were attracted to both rough rice and could not distinguish the transgenic TIC-19 from the isogenic rough rice. These results demonstrated that Bt rough rice has no negative impacts on the host preference behaviors of non-target stored product pest R. dominica and its parasitoid A. calandrae. (C) 2015 Elsevier Inc. All rights reserved. C1 [Sun, Xiao; Yan, Miao-Jun; Wang, Man-Qun] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Zhang, Aijun] USDA ARS, Invas Insect Biocontrol & Behav Lab, BARC West, Beltsville, MD 20705 USA. RP Wang, MQ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. EM mqwang@mail.hzau.edu.cn FU National Genetically Modified Organisms Breeding Major Projects of China [2011ZZX08001-001]; Fundamental Research Funds for the Central Universities [2013PY046] FX This study was supported and funded by the National Genetically Modified Organisms Breeding Major Projects of China (No. 2011ZZX08001-001) and Fundamental Research Funds for the Central Universities (2013PY046). NR 59 TC 1 Z9 1 U1 1 U2 28 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD OCT PY 2015 VL 120 BP 449 EP 456 DI 10.1016/j.ecoenv.2015.06.034 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA CO3AQ UT WOS:000359029000058 PM 26150137 ER PT J AU Snider, JL Chastain, DR Coillins, GD Grey, TL Sorensen, RB AF Snider, John L. Chastain, Daryl R. Coillins, Guy D. Grey, Timothy L. Sorensen, Ronald B. TI Do genotypic differences in thermotolerance plasticity correspond with water-induced differences in yield and photosynthetic stability for field-grown upland cotton? SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Heat tolerance; Photosystem II; Gossypium hirsutum; Cotton; Lint yield; Plasticity ID GOSSYPIUM-HIRSUTUM; ELECTRON-TRANSPORT; PHOTOSYSTEM-II; HEAT-STRESS; RUBISCO ACTIVASE; HIGH-TEMPERATURE; PIMA COTTON; PLANTS; FLUORESCENCE; TOLERANCE AB To determine if cultivar differences in thermotolerance plasticity of photosystem II promote yield or photosynthetic stability when variability in both parameters is water-induced, the temperature response of maximum quantum yield of photosystem II (F-v/F-m) was evaluated for two cotton cultivars (FM 1944 GLB2 and PHY 499 WRF), at three times (squaring, first flower, and peak bloom) during the 2014 growing season, under five different irrigation regimes. The temperature inducing a 15% decline in F-v/F-m from the optimum (T-15) served as a measure of thermotolerance. Furthermore, during 2014 net photosynthesis (A(N)) measurements coincided with T-15 measurements; predawn water potential (Psi(PD)) was measured three days per week; and canopy temperature (T-C) was measured continuously. Yield was determined for both cultivars and all five irrigation regimes in 2013 and 2014 although significant irrigation effects were only observed during 2014. Thermotolerance, photosynthetic, and yield stability were determined using a regression approach to assess genotype x environment interaction. Irrigation treatments significantly affected Psi(PD) and T-C and large variability in thermotolerance (although all T-15 values were well-above the observed air and canopy temperatures), A(N), and lint yield were observed. The most thermotolerance-stable cultivar was also the most photosynthetically stable and yield stable, suggesting that greater thermotolerance plasticity of PSII does not necessarily promote yield stability when yield variability is water-induced. Also, the methods described here provide an approach to rapidly assess thermotolerance plasticity of field grown cotton that could be adopted on a large scale and that does not require extensive knowledge of site-specific conditions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Snider, John L.; Chastain, Daryl R.; Grey, Timothy L.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31794 USA. [Coillins, Guy D.] N Carolina State Univ, Dept Crop Sci, Upper Coastal Plains Res Stn, Rocky Mount, NC 27801 USA. [Sorensen, Ronald B.] ARS, USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA. RP Snider, JL (reprint author), Univ Georgia, Dept Crop & Soil Sci, 115 Coastal Way, Tifton, GA 31794 USA. EM jlsnider@uga.edu FU Georgia Cotton Commission; Cotton Incorporated FX The authors thank the Georgia Cotton Commission and Cotton Incorporated for providing financial support of this project and the University of Georgia for research facilities. We also thank Lola Sexton, Dudley Cook, Calvin Perry, Will Vance, Jenna Pitts, Tyler Beasley, and Keri Dixon for their assistance in the field and Andy Knowlton for sample ginning. NR 37 TC 1 Z9 1 U1 0 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD OCT PY 2015 VL 118 BP 49 EP 55 DI 10.1016/j.envexpbot.2015.06.005 PG 7 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA CO3AL UT WOS:000359028500006 ER PT J AU Wang, LB Baldwin, EA Plotto, A Luo, WQ Raithore, S Yu, ZF Bai, JH AF Wang, Libin Baldwin, Elizabeth A. Plotto, Anne Luo, Weiqi Raithore, Smita Yu, Zhifang Bai, Jinhe TI Effect of methyl salicylate and methyl jasmonate pre-treatment on the volatile profile in tomato fruit subjected to chilling temperature SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Solanum lycopersicum; Aroma; Ripening; Chilling injury; Sensory ID ARGININE CATABOLISM; GENE-EXPRESSION; FLAVOR; INJURY; ACID; BIOSYNTHESIS; ACCUMULATION; QUALITY; AROMA; BIOCHEMISTRY AB Tomato fruits exposed to chilling temperatures suffer aroma loss prior to visual chilling. injury (CI) symptoms. Methyl salicylate (MeSA) and methyl jasmonate (MeJA) treatments were reported to alleviate the development of visual Cl; however, it is unknown if the treatments alleviate internal Cl in the form of aroma loss. In this research, 'FL 47' tomatoes at breaker stage were treated with MeSA or MeJA vapor prior exposure to chilling temperature. The chilling treatment did not result in visual Cl; however, for internal Cl it generally suppressed production of oxygen-containing heterocyclic compounds, ketones, sulfur- and nitrogen-containing heterocyclic compounds, alcohols, and aldehydes, including 13 important aroma contributors to tomato fruit. MeJA had no impact on sensory evaluation in spite of resulting in slightly altered volatile profile; however, MeSA alleviated the Cl-induced reduction of a number of volatile compounds, and thereby enhanced tomato aroma. Published by Elsevier B.V. C1 [Wang, Libin; Baldwin, Elizabeth A.; Plotto, Anne; Luo, Weiqi; Raithore, Smita; Bai, Jinhe] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Wang, Libin; Yu, Zhifang] Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. RP Bai, JH (reprint author), ARS, USDA, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov FU China Scholarship Council [201306850049]; Postgraduate Program in Jiangsu Province [CXLX13-267]; Big Red Tomato Packers, Fort Pierce, FL FX We wish to thank the financial support to the experiment from China Scholarship Council (201306850049), Postgraduate Program in Jiangsu Province (CXLX13-267) and the Big Red Tomato Packers, Fort Pierce, FL. NR 51 TC 8 Z9 9 U1 8 U2 59 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD OCT PY 2015 VL 108 BP 28 EP 38 DI 10.1016/j.postharvbio.2015.05.005 PG 11 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CO2GA UT WOS:000358973000004 ER PT J AU Listopad, CMCS Masters, RE Drake, J Weishampel, J Branquinho, C AF Listopad, Claudia M. C. S. Masters, Ronald E. Drake, Jason Weishampel, John Branquinho, Cristina TI Structural diversity indices based on airborne LiDAR as ecological indicators for managing highly dynamic landscapes SO ECOLOGICAL INDICATORS LA English DT Article DE Ecological indicator; Fire regime; LiDAR remote sensing; Pine-grassland; Shannon height diversity index; Structural biodiversity ID LARGE-FOOTPRINT LIDAR; CANOPY STRUCTURE; FOREST STRUCTURE; SPECIES RICHNESS; RETURN LIDAR; RAIN-FOREST; STAND; FLORIDA; BIRD; FIRE AB An objective, quantifiable index of structural biodiversity that could be rapidly obtained with reduced or no field effort is essential for the use of structure as universal ecological indicator for ecosystem management. Active remote sensing provides a rapid assessment tool to potentially guide land managers in highly dynamic and spatially complex landscapes. These landscapes are often dependent on frequent disturbance regimes and characterized by high endemism. We propose a modified Shannon-Wiener Index and modified Evenness Index as stand structural complexity indices for surrogates of ecosystem health. These structural indices are validated at Tall Timbers Research Station the site of one of the longest running fire ecology studies in southeastern U.S. This site is dominated by highly dynamic pine-grassland woodlands maintained with frequent fire. Once the dominant ecosystem in the Southeast, this woodland complex has been cleared for agriculture or converted to other cover types, and depends on a frequent (1- to 3-year fire return interval) low- to moderate-intensity fire regime to prevent succession to mixed hardwood forests and maintain understory species diversity. Structural evaluation of the impact of multiple disturbance regimes included height profiles and derived metrics for five different fire interval treatments; 1-year, 2-year, 3-year, mixed fire frequency (a combination of 2- and 4-year fire returns), and fire exclusion. The 3-dimensional spatial arrangement of structural elements was used to assess hardwood encroachment and changes in structural complexity. In agreement with other research, 3-year fire return interval was considered to be the best fire interval treatment for maintaining the pine-grassland woodlands, because canopy cover and vertical diversity indices were shown to be statistically higher in fire excluded and less frequently burned plots than in 1- and 2-year fire interval treatments. We developed a LiDAR-derived structural diversity index, LHDI, and propose that an ecosystem-specific threshold target for management intervention can be developed, based on significant shifts in structure and composition using this new index. Structural diversity indices can be valuable surrogates of ecosystem biodiversity, and ecosystem-specific target values can be developed as objective quantifiable goals for conservation and ecosystem integrity, particularly in remote areas. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Listopad, Claudia M. C. S.; Weishampel, John] Univ Cent Florida, Dept Biol, Orlando, FL 32816 USA. [Masters, Ronald E.] Univ Wisconsin, Stevens Point, WI 54481 USA. [Drake, Jason] US Forest Serv, USDA, Tallahassee, FL USA. [Listopad, Claudia M. C. S.; Branquinho, Cristina] Univ Lisbon, Fac Ciencias, Ctr Biol Ambiental, FCUL, P-1749016 Lisbon, Portugal. RP Listopad, CMCS (reprint author), 1852 Cato Court, Indialantic, FL 32903 USA. EM cdlistopad@fc.ul.pt; Ron.Masters@uwsp.edu; jasondrake@fs.fed.us; John.Weishampel@ucf.edu; cmbranquinho@fc.ul.pt RI Branquinho, Cristina/B-3670-2008 OI Branquinho, Cristina/0000-0001-8294-7924 FU NASA New Investigator Program [NG04GO52G]; FCT-MCTES grant [SFRH/BPD/78679/2011] FX This research was financially supported by NASA New Investigator Program grant (NG04GO52G) and FCT-MCTES grant (SFRH/BPD/78679/2011). NR 48 TC 6 Z9 6 U1 12 U2 100 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD OCT PY 2015 VL 57 BP 268 EP 279 DI 10.1016/j.ecolind.2015.04.017 PG 12 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CN0GJ UT WOS:000358091800032 ER PT J AU Munther, D Luo, YG Wu, JH Magpantay, FMG Srinivasan, P AF Munther, Daniel Luo, Yaguang Wu, Jianhong Magpantay, Felicia M. G. Srinivasan, Parthasarathy TI A mathematical model for pathogen cross-contamination dynamics during produce wash SO FOOD MICROBIOLOGY LA English DT Article DE Mathematical modeling; Cross-contamination; Produce wash-cycle ID ESCHERICHIA-COLI O157/H7; CUT LEAFY GREENS; E.-COLI; CHLORINE; WATER; EFFICACY; LETTUCE; INACTIVATION; SALMONELLA; EQUIPMENT AB One of the main challenges for the fresh-food produce industry is to ensure that the produce is free from harmful pathogens. A potential area of risk is due to cross-contamination in a sanitizing chlorine wash-cycle, where the same water is used to wash contaminated as well as non-contaminated produce. However, this is also an area where effective intervention strategies are possible, provided we have a good understanding of the mechanism of cross-contamination. Based on recent experimental work by Luo, Y. et al. A pilot plant scale evaluation of a new process aid for enhancing chlorine efficacy against pathogen survival and cross-contamination during produce wash, International Journal of Food Microbiology, 158 (2012), 133-139, we have built mathematical models that allow us to quantify the amount of cross-contamination of Escherichia coli O157:H7 from spinach to lettuce, and assessed the efficacy of the associated wash-cycle protocols. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Munther, Daniel; Srinivasan, Parthasarathy] Cleveland State Univ, Dept Math, Cleveland, OH 44115 USA. [Luo, Yaguang] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD USA. [Wu, Jianhong] York Univ, Dept Math & Stat, Toronto, ON M3J 2R7, Canada. [Magpantay, Felicia M. G.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. RP Srinivasan, P (reprint author), Cleveland State Univ, Dept Math, Cleveland, OH 44115 USA. EM p.srinivasan35@csuohio.edu NR 12 TC 0 Z9 1 U1 4 U2 34 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD OCT PY 2015 VL 51 BP 101 EP 107 DI 10.1016/j.fm.2015.05.010 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CM9FP UT WOS:000358013100014 PM 26187834 ER PT J AU Gashua, IB Williams, PA Yadav, MP Baldwin, TC AF Gashua, I. B. Williams, P. A. Yadav, M. P. Baldwin, T. C. TI Characterisation and molecular association of Nigerian and Sudanese Acacia gum exudates SO FOOD HYDROCOLLOIDS LA English DT Article DE Gum Arabic; Molecular association; Acacia gum ID SENEGAL GUM; PROTEIN; ARABINOGALACTAN; WEIGHT; FRACTION AB The chemical and physicochemical characteristics of gum exudate samples harvested from mature trees of Acacia senegal at two new specific ecolocations in Nigeria, have been investigated together with gum samples harvested from A. senegal and A. seyal originating from Sudan. The monosaccharide sugar compositions for the A. senegal gum samples were found to be similar, but the protein contents for the Nigerian samples were significantly higher than recorded for the Sudanese sample. Gel Permeation Chromatography coupled to light scattering, refractive index and U.V. detectors, has shown the presence of arabinogalactan, arabinogalactan-protein and glycoprotein fractions within the A. senegal gums and has also shown the presence of an additional small proportion of very low molar mass proteinaceous material all the samples which has previously been ignored. The plot of radius of gyration, R-g, as a function of elution volume showed a discontinuity for one of the Nigerian samples and for the A. seyal gum sample at elution volumes corresponding to the AGP component suggesting a different molecular structure. Plots of Mw-v-R-g confirmed that the molecules had a compact structure. The hydrodynamic size of the molecules was followed using dynamic light scattering as a function of time and it was found that molecular association occurred in solution. The extent of association increased as the protein content in the sample increased and was inhibited in the presence of electrolyte, it was concluded that association was due to electrostatic interaction between the protein moieties and glucuronic acid groups on individual macromolecules. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Gashua, I. B.; Baldwin, T. C.] Wolverhampton Univ, Fac Sci & Engn, Wolverhampton WV1 1LY, W Midlands, England. [Gashua, I. B.; Williams, P. A.] Glyndwr Univ, Ctr Water Soluble Polymers, Wrexham LL11 2AW, Wales. [Gashua, I. B.] Fed Polytech Damaturu, Dept Sci Lab Technol, Damaturu, Yobe State, Nigeria. [Yadav, M. P.] USDA, Eastern Reg Res Ctr, Agr Res Serv, Wyndmoor, PA 19038 USA. RP Baldwin, TC (reprint author), Wolverhampton Univ, Fac Sci & Engn, Wulfruna St, Wolverhampton WV1 1LY, W Midlands, England. EM T.Baldwin@wlv.ac.uk OI Williams, Peter A./0000-0002-5744-3430 FU Federal Polytechnic Damaturu; Tertiary Education Trust Fund; Staff training intervention Nigeria FX Ibrahim B. Gashua is grateful to The Federal Polytechnic Damaturu, and the Tertiary Education Trust Fund, 2011/12 Staff training intervention Nigeria for providing sponsorship in order to carry out this work. NR 28 TC 5 Z9 5 U1 1 U2 37 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD OCT PY 2015 VL 51 BP 405 EP 413 DI 10.1016/j.foodhyd.2015.05.037 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CM8DV UT WOS:000357928200043 ER PT J AU Yi, J Li, Y Zhong, F Yokoyama, W AF Yi, Jiang Li, Yue Zhong, Fang Yokoyama, Wallace TI The physicochemical stability and in vitro bioaccessibility of beta- carotene in oil- in-water sodium caseinate emulsions (vol 35, pg 19, 2014) SO FOOD HYDROCOLLOIDS LA English DT Correction C1 [Yi, Jiang; Li, Yue; Zhong, Fang] Jiangnan Univ, Key Lab Food Colloids & Biotechnol, Minist Educ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China. [Yi, Jiang; Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Yokoyama, W (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM fzhong@jiangnan.edu.cn; Wally.Yokoyama@ARS.USDA.GOV NR 1 TC 0 Z9 0 U1 6 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD OCT PY 2015 VL 51 BP 519 EP 519 DI 10.1016/j.foodhyd.2015.05.005 PG 1 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CM8DV UT WOS:000357928200056 ER PT J AU Liu, JY Sidhu, SS Wang, ML Tonnis, B Habteselassie, M Mao, JD Huang, QG AF Liu, Jiayang Sidhu, Sudeep S. Wang, Ming Li Tonnis, Brandon Habteselassie, Mussie Mao, Jingdong Huang, Qingguo TI Evaluation of various fungal pretreatment of switchgrass for enhanced saccharification and simultaneous enzyme production SO JOURNAL OF CLEANER PRODUCTION LA English DT Article DE Bioethanol; Switchgrass biomass; Fungal pretreatment; Enzymatic hydrolysis; Enzyme production; Accessory enzyme ID DILUTE-ACID PRETREATMENT; LIGNOCELLULOSIC BIOMASS; RICE STRAW; PHANEROCHAETE-CHRYSOSPORIUM; BIOETHANOL PRODUCTION; ETHANOL-PRODUCTION; WHITE-ROT; HYDROLYSIS; LACCASE; FERMENTATION AB In order to produce clean energy from renewable lignocellulosic biomass, the feedstock must be pretreated by means of one or combined cost-competitive methods to make the entire process economically feasible. Fungal cultivation may be one promising pretreatment approach, for which the effectiveness varies with different fungal strains in regard to biomass loss, sugar yield, enzyme loading, and co-product yield. Wild-type fungus might have a great potential in pretreating feedstock for both lowered enzyme loading and beneficial co-product enzyme production. In this study, 25 diverse fungal strains were screened for pretreatment of switchgrass biomass with regard to co-production of beneficial enzymes and the subsequent saccharification process, by examining switchgrass biomass loss, effectiveness of enzymatic hydrolysis, and ligninolytic activity. The most promising fungus was identified as an inedible mushroom, Agaricus molybdites (H4) with the highest saccharification efficiency and ligninolytic enzyme production. Additionally, eight accessory enzymes, e.g. pectinase, esterase, xylanase, beta-glucosidase, chitinase, lipase, a-amylase, and laccase, were found to assist cellulase in enhancing saccharification of H4 pretreated switchgrass to varying degrees. Our experimental results suggest that pretreatment of switchgrass with an appropriate fungus, followed by exposure to cellulase and synergistic accessory enzymes can be an effective strategy for maximizing the process of saccharification. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Liu, Jiayang; Sidhu, Sudeep S.; Habteselassie, Mussie; Huang, Qingguo] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. [Liu, Jiayang] Huanghuai Univ, Sch Bioengn, Zhumadian 463000, Peoples R China. [Wang, Ming Li; Tonnis, Brandon] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Mao, Jingdong] Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. RP Huang, QG (reprint author), Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. EM qhuang@uga.edu FU HATCH funds FX This work was supported in part by HATCH funds. The authors thank Dr. Melanie Harrison-Dunn for providing switchgrass materials. NR 42 TC 5 Z9 5 U1 8 U2 50 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0959-6526 EI 1879-1786 J9 J CLEAN PROD JI J. Clean Prod. PD OCT 1 PY 2015 VL 104 BP 480 EP 488 DI 10.1016/j.jclepro.2015.04.094 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA CM3AG UT WOS:000357552900048 ER PT J AU Ontl, TA Cambardella, CA Schulte, LA Kolka, RK AF Ontl, Todd A. Cambardella, Cynthia A. Schulte, Lisa A. Kolka, Randall K. TI Factors influencing soil aggregation and particulate organic matter responses to bioenergy crops across a topographic gradient SO GEODERMA LA English DT Article DE Carbon; Organic matter; Soil; Physical protection; Aggregation; Bioenergy ID ARBUSCULAR MYCORRHIZAL FUNGI; NATIVE GRASSLAND SOILS; ROOT-DERIVED CARBON; NO-TILLAGE SOILS; LAND-USE; CONVENTIONAL-TILLAGE; LANDSCAPE POSITION; DENSITY FRACTIONS; CROPPING SYSTEMS; SILTY SOILS AB Bioenergy crops have the potential to enhance soil carbon (C) pools from increased aggregation and the physical protection of organic matter; however, our understanding of the variation in these processes over heterogeneous landscapes is limited. In particular, little is known about the relative importance of soil properties and root characteristics for the physical protection of particulate organic matter (POM). We studied short-term (3-year) changes in aggregation and POM-C pools under three cropping systems (switchgrass, a triticale/sorghum double crop, continuous corn) replicated across five landscape positions along a topographic gradient in Iowa, USA. We isolated POM associated with three aggregate fractions (>2 mm, 025-2 mm, and 0.053-0.25 mm) to determine the relative influence of ten soil and three root properties. Aggregation increased in all cropping systems and was greatest under switchgrass; however cropping system effects were not consistent among positions. Total soil organic C stocks did not change, but C within both physically protected (iPOM-C) and unprotected (frPOM) C pools increased. Shifts in iPOM-C were concurrently influenced by soil properties and root traits. Soil texture had the strongest influence (65% relative importance), with finer-textured soils showing greater gains in total iPOM-C, while greater root biomass influenced (35% relative importance) accrual of total iPOM-C. Aggregate fractions varied in their iPOM-C response to soil and root variables, however individual pools similarly showed the importance of soil texture and root biomass and annual root productivity (BNPP). Changes in frPOM-C were strongly correlated with BNPP. Our data suggest that macroaggregate formation drives short-term responses of POM, which are influenced by both soil and root system properties. Crops that maximize root biomass and BNPP will lead to the largest increases in protected soil C stocks. However, C storage rates will vary across landscapes according to soil conditions, with texture as the primary influence. (C) 2015 Elsevier B.V. All rights reserved. C1 [Ontl, Todd A.; Schulte, Lisa A.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Cambardella, Cynthia A.] USDA Agr Res Serv, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Kolka, Randall K.] USDA Forest Serv, Northern Res Stn, Grand Rapids, MN 55744 USA. RP Ontl, TA (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49930 USA. EM taontl@gmail.com OI Ontl, Todd/0000-0003-4036-4848 NR 101 TC 4 Z9 4 U1 8 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD OCT PY 2015 VL 255 BP 1 EP 11 DI 10.1016/j.geoderma.2015.04.016 PG 11 WC Soil Science SC Agriculture GA CL0OP UT WOS:000356642300001 ER PT J AU Lin, BF Luo, YG Teng, Z Zhang, BC Zhou, B Wang, Q AF Lin, Baofeng Luo, Yaguang Teng, Zi Zhang, Boce Zhou, Bin Wang, Qin TI Development of silver/titanium dioxide/chitosan adipate nanocomposite as an antibacterial coating for fruit storage SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Silver; Titanium dioxide; Chitosan adipate; Nanocomposite; Antibacterial coating ID WATER-SOLUBLE CHITOSAN; SILVER NANOPARTICLES; ANTIMICROBIAL ACTIVITY; MULTILAYERED FILMS; GREEN SYNTHESIS; IRRADIATION; REDUCTION; TOXICITY; AGENT; TIO2 AB A novel nanocomposite of silver/titanium dioxide/chitosan adipate (Ag/TiO2/CS) was developed through photochemical reduction using a chitosan adipate template. Chitosan served as a reducing agent anchoring the metal ions through Ag-N coordination bonds and electrostatic attractions, thus stabilizing the Ag/TiO2/CS product observed by Fourier transform infrared spectroscopy and X-ray diffraction patterns. Scanning electron microscopy observations revealed that the nanocomposite particles (50-100 nm) were deposited onto the chitosan adipate layer. The product exhibited high zeta-potentials from 30.1 mV to 33.0 mV during 60 days of storage. In addition, the nanocomposite demonstrated higher antimicrobial activity than AgNO3 or nano-Ag particles at similar concentrations, as evidenced by the inhibition zone, minimum inhibitory concentrations (MIC), and growth curve. The nanocomposite reduced the Escherichia coli population by 6 logs after 24 h of incubation, and had an MIC value of 038 ug Ag/mL. These results suggest that Ag/TiO2/CS has the potential to be used as an antibacterial protective coating for fruit storage. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Lin, Baofeng] Guangxi Univ, Coll Chem & Chem Engn, Nanning 530004, Peoples R China. [Lin, Baofeng; Luo, Yaguang; Zhang, Boce; Zhou, Bin] ARS, Food Qual Lab, USDA, Beltsville, MD 20705 USA. [Teng, Zi; Zhang, Boce; Zhou, Bin; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Luo, YG (reprint author), ARS, Food Qual Lab, USDA, Beltsville, MD 20705 USA. EM Yaguang.Luo@ars.usda.gov RI Teng, Zi/G-5747-2013 OI Teng, Zi/0000-0002-6029-7024 FU USDA-NIFA Specialty Crop Research Initiative Grant Award [2010-51181-21230]; National Natural Science Foundation of China [51263001] FX This research is supported by the USDA-NIFA Specialty Crop Research Initiative Grant Award No. 2010-51181-21230, and by National Natural Science Foundation of China under Grant No.51263001(to Baofeng Lin). We are grateful for the technical support of the Maryland Nano Center of the University of Maryland, and to Ms. Ellen Turner at USDA-ARS. NR 40 TC 2 Z9 3 U1 9 U2 104 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD OCT PY 2015 VL 63 IS 2 BP 1206 EP 1213 DI 10.1016/j.lwt.2015.04.049 PG 8 WC Food Science & Technology SC Food Science & Technology GA CK9FH UT WOS:000356545500058 ER PT J AU Mukhopadhyay, S Ukuku, DO Juneja, VK AF Mukhopadhyay, Sudarsan Ukuku, Dike O. Juneja, Vijay K. TI Effects of integrated treatment of nonthermal UV-C light and different antimicrobial wash on Salmonella enterica on plum tomatoes SO FOOD CONTROL LA English DT Article DE Salmonella enterica; Tomatoes; Antimicrobials; Ultraviolet light ID ESCHERICHIA-COLI O157H7; ACIDIC ELECTROLYZED WATER; HIGH HYDROSTATIC-PRESSURE; AQUEOUS CHLORINE DIOXIDE; HYDROGEN-PEROXIDE; ORGANIC-ACIDS; RAW TOMATOES; LISTERIA-MONOCYTOGENES; CARDIOVASCULAR-DISEASE; REDUCING SALMONELLA AB The aim of this study was to evaluate inactivation of inoculated Salmonella enterica on whole tomato surface exploiting integration of nonthermal ultraviolet light (UV-C) treatment with antimicrobial wash. The effect of combined treatment on background microflora (aerobic mesophilic, and yeast and mold), during storage at ambient temperature (22 degrees C) for 21 days was also determined. A bacterial cocktail containing three serotypes of S. enterica (S. Newport H1275, S. Stanley H0558, and S. Montevideo G4639) was used based on their association with produce-related outbreaks. Tomatoes were spot inoculated using approximately 100 mu L of inocula to achieve cell population of about 10(7) CFU/tomato. An inoculated tomato was initially treated with a low (0.6 kJ/m(2)) dose of UV-C light (253.7 nm) followed by immersion in selected sanitizing solution (700 ml) to wash under mild agitation (ca. 250 rpm) for 2 min at room temperature (22 degrees C). Inactivation efficacy of combined treatments varied widely depending on the sanitizer property. Combined UV-C plus aqueous ozone (1 ppm) provided 3.13 +/- 0.47 log CPU/fruit Salmonella reduction which was significantly lower (p < 0.05) compared to the rest of the combination treatments; whereas the treatment of UV-C followed by immersion in a novel antimicrobial preparation 'HEN', formulated mixing hydrogen peroxide, EDTA and nisin provided the best log reduction (4.71 +/- 0.25 log CFU/fruit). Organic acids (1%) or their binary mixtures, hydrogen peroxide (3%), and HEN provided greater than 4.0 log reductions for UV-C treated tomatoes. Treatments were effective in controlling native microbial loads as the total aerobic mesophilic organisms and the population of yeast and mold remained significantly (p < 0.05) low during storage compared to control. Findings from this study provide safe and effective post harvest intervention strategies for produce industry as an alternative to current chlorine based wash. These results may also help researchers design future decontamination studies. Published by Elsevier Ltd. C1 [Mukhopadhyay, Sudarsan; Juneja, Vijay K.] ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Ukuku, Dike O.] ARS, Food Safely Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Mukhopadhyay, S (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM Sudarsan.mukhopadhyay@ars.usda.gov; Dike.ukuku@ars.usda.gov; Vijay.juneja@ars.usda.gov NR 61 TC 1 Z9 1 U1 5 U2 41 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD OCT PY 2015 VL 56 BP 147 EP 154 DI 10.1016/j.foodcont.2015.03.020 PG 8 WC Food Science & Technology SC Food Science & Technology GA CK0HN UT WOS:000355886400021 ER PT J AU Tao, FF Peng, YK Gomes, CL Chao, KL Qin, JW AF Tao, Feifei Peng, Yankun Gomes, Carmen L. Chao, Kuanglin Qin, Jianwei TI A comparative study for improving prediction of total viable count in beef based on hyperspectral scattering characteristics SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Beef; Total viable count; Hyperspectral scattering imaging; Nondestructive detection; Lorentzian function; Parameter combination ID TRANSFORM INFRARED-SPECTROSCOPY; QUANTITATIVE DETECTION; MEAT SPOILAGE; MICROBIAL SPOILAGE; IMAGING TECHNIQUE; LIGHT-SCATTERING; NEURAL-NETWORK; SOLUBLE SOLIDS; PORK MEAT; TENDERNESS AB The objectives of this study were to investigate the feasibility of hyperspectral scattering imaging to predict the bacterial contamination in meat nondestructively, and propose an optimal approach for detecting low levels of total viable count (TVC) contamination in beef. Fresh beef samples were obtained from a commercial slaughtering plant, and stored at 4 degrees C for 0-12 days. The visible/near-infrared (VIS/NIR) hyperspectral images in the backscattering mode were acquired from 3-5 beef samples on each day of the experiment, in parallel with microbiological analysis to enumerate the TVC population. Lorentzian function was used to resolve the light scattering information within the hyperspectral image and consequently Lorentzian parameters, which represented different hyperspectral scattering characteristics were extracted. In this study, not only the individual Lorentzian parameters but also the parameter combinations were used to establish the multivariate statistical models for predicting beef TVC, based on the modeling methods of principal component regression (PCR), partial least squares regression (PLSR), and back propagation neural network (BPNN), respectively. The models established using individual Lorentzian parameters did not perform well in predicting low levels of TVC contamination in beef, and the best prediction result could only achieved with the correlation coefficient of prediction set (R-p) and root mean squared error of prediction set (RMSEP) of 0.81 and 1.27 log CFU/g, respectively. Based on the parameter combinations, the best modeling results were achieved with R-p and RMSEP of 0.86 and 0.93 log CFU/g, 0.87 and 0.79 log CFU/g, 0.90 and 0.88 log CFU/g by PCR, PLSR, and BPNN methods, respectively, which confirmed the superiority of the parameter combination method. The results of this study demonstrated for the first time that hyperspectral scattering imaging combined with Lorentzian function and the proposed parameter combination method could be used to detect low levels of bacterial contamination in beef nondestructively. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Tao, Feifei; Peng, Yankun] China Agr Univ, Coll Engn, Beijing 100083, Peoples R China. [Gomes, Carmen L.] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Chao, Kuanglin; Qin, Jianwei] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. RP Peng, YK (reprint author), China Agr Univ, Coll Engn, POB 102,17 Qinghua East Rd, Beijing 100083, Peoples R China. EM ypeng@cau.edu.cn RI Gomes, Carmen/J-5091-2015 OI Gomes, Carmen/0000-0003-0095-6478 FU Special Fund for Agro-scientific Research in the Public Interest Program [201003008]; National Science and Technology Support Program [2012BAH04B00]; China Postdoctoral Science Foundation [2014M561096] FX The authors gratefully acknowledge the Special Fund for Agro-scientific Research in the Public Interest Program (Project No. 201003008), the National Science and Technology Support Program (Project No. 2012BAH04B00), and the China Postdoctoral Science Foundation (Project No. 2014M561096) for supporting this research. NR 42 TC 3 Z9 4 U1 10 U2 50 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD OCT PY 2015 VL 162 BP 38 EP 47 DI 10.1016/j.jfoodeng.2015.04.008 PG 10 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA CJ4ZC UT WOS:000355496200006 ER PT J AU Yang, SP Wang, Y Beier, RC Zhang, HY De Ruyck, K Sun, FF Cao, XY Shen, JZ Zhang, SX Wang, ZH AF Yang, Shupeng Wang, Ying Beier, Ross C. Zhang, Huiyan De Ruyck, Karl Sun, Feifei Cao, Xingyuan Shen, Jianzhong Zhang, Suxia Wang, Zhanhui TI Simultaneous Determination of Type A and B Trichothecenes and Their Main Metabolites in Food Animal Tissues by Ultraperformance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE type A and B trichothecenes; DON; T-2 toxin; metabolites; chicken; pork; liver; liquid chromatography; tandem mass spectrometry ID LC-MS/MS METHOD; T-2 TOXIN; MYCOTOXINS; DEOXYNIVALENOL; VALIDATION; EXTRACTION; WHEAT; PATHWAYS; EXPOSURE; URINE AB A rapid method for the liquid chromatography tandem mass spectrometric determination of type A and B trichothecenes and their major metabolites in chicken meat, pork, chicken liver, and swine liver was developed. The analytes included T-2 toxin, HT-2 toxin, T-2 triol, neosolaniol, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deepoxydeoxynivalenol, and nivalenol. The compounds were extracted from samples with acetonitrile/ethyl acetate (1:3, v/v) and then cleaned up using Oasis HLB cartridges. Analysis was carried out with ultraperformance liquid chromatography tandem mass spectrometry. The mean recoveries of spiked samples ranged from 74.1% to 96.9% with intraday and interday relative standard deviations of less than 9.9% and 9.1%, respectively. The limit of detection and limit of quantitation ranged from 3.0 to 15.0 mu g/kg and from 10.0 to 50.0 mu g/kg, respectively. The proposed method has been successfully applied for analysis of real samples, with the primary results indicating that, compared to mycotoxins themselves, their metabolites are more likely to occur and be detectable in animal tissue foods. C1 [Yang, Shupeng; Wang, Ying; Zhang, Huiyan; Sun, Feifei; Cao, Xingyuan; Shen, Jianzhong; Zhang, Suxia; Wang, Zhanhui] China Agr Univ, Beijing Key Lab Detect Technol Anim Derived Food, Natl Reference Lab Vet Drug Residues, Beijing Lab Food Qual & Safety,Coll Vet Med, Beijing 100193, Peoples R China. [Beier, Ross C.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. [De Ruyck, Karl] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, B-9000 Ghent, Belgium. RP Zhang, SX (reprint author), China Agr Univ, Beijing Key Lab Detect Technol Anim Derived Food, Natl Reference Lab Vet Drug Residues, Beijing Lab Food Qual & Safety,Coll Vet Med, Beijing 100193, Peoples R China. EM suxia@cau.edu.cn FU National Natural Science Foundation of China (NSFC) [U1301214]; International Science & Technology Cooperation Program of China [2012DFG31840]; Special Fund for Agro-scientific Research in the Public Interest [201203040] FX This work was supported by the National Natural Science Foundation of China (NSFC) (Grant U1301214), the International Science & Technology Cooperation Program of China (Grant 2012DFG31840), and the Special Fund for Agro-scientific Research in the Public Interest (Grant 201203040). NR 30 TC 4 Z9 4 U1 1 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD SEP 30 PY 2015 VL 63 IS 38 BP 8592 EP 8600 DI 10.1021/acs.jafc.5b03281 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CS7DA UT WOS:000362243100029 PM 26321427 ER PT J AU Yu, DH Gadkari, M Zhou, Q Yu, SY Gao, N Guan, YT Schady, D Roshan, TN Chen, MH Laritsky, E Ge, ZQ Wang, H Chen, R Westwater, C Bry, L Waterland, RA Moriarty, C Hwang, C Swennes, AG Moore, SR Shen, LL AF Yu, Da-Hai Gadkari, Manasi Zhou, Quan Yu, Shiyan Gao, Nan Guan, Yongtao Schady, Deborah Roshan, Tony N. Chen, Miao-Hsueh Laritsky, Eleonora Ge, Zhongqi Wang, Hui Chen, Rui Westwater, Caroline Bry, Lynn Waterland, Robert A. Moriarty, Chelsea Hwang, Cindy Swennes, Alton G. Moore, Sean R. Shen, Lanlan TI Postnatal epigenetic regulation of intestinal stem cells requires DNA methylation and is guided by the microbiome SO GENOME BIOLOGY LA English DT Article ID CPG ISLAND METHYLATION; EPITHELIAL-CELLS; GUT MICROBIOME; CANCER-CELLS; GENE-CONTROL; GERM-FREE; DIFFERENTIATION; MICE; GLYCOSYLATION; METABOLISM AB Background: DNA methylation is an epigenetic mechanism central to development and maintenance of complex mammalian tissues, but our understanding of its role in intestinal development is limited. Results: We use whole genome bisulfite sequencing, and find that differentiation of mouse colonic intestinal stem cells to intestinal epithelium is not associated with major changes in DNA methylation. However, we detect extensive dynamic epigenetic changes in intestinal stem cells and their progeny during the suckling period, suggesting postnatal epigenetic development in this stem cell population. We find that postnatal DNA methylation increases at 3' CpG islands (CGIs) correlate with transcriptional activation of glycosylation genes responsible for intestinal maturation. To directly test whether 3' CGI methylation regulates transcription, we conditionally disrupted two major DNA methyltransferases, Dnmt1 or Dnmt3a, in fetal and adult intestine. Deficiency of Dnmt1 causes severe intestinal abnormalities in neonates and disrupts crypt homeostasis in adults, whereas Dnmt3a loss was compatible with intestinal development. These studies reveal that 3' CGI methylation is functionally involved in the regulation of transcriptional activation in vivo, and that Dnmt1 is a critical regulator of postnatal epigenetic changes in intestinal stem cells. Finally, we show that postnatal 3' CGI methylation and associated gene activation in intestinal epithelial cells are significantly altered by germ-free conditions. Conclusions: Our results demonstrate that the suckling period is critical for epigenetic development of intestinal stem cells, with potential important implications for lifelong gut health, and that the gut microbiome guides and/or facilitates these postnatal epigenetic processes. C1 [Yu, Da-Hai; Gadkari, Manasi; Zhou, Quan; Guan, Yongtao; Roshan, Tony N.; Chen, Miao-Hsueh; Laritsky, Eleonora; Waterland, Robert A.; Moriarty, Chelsea; Hwang, Cindy; Shen, Lanlan] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Yu, Shiyan; Gao, Nan] Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA. [Guan, Yongtao; Waterland, Robert A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Schady, Deborah] Texas Childrens Hosp, Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA. [Ge, Zhongqi; Wang, Hui; Chen, Rui] Baylor Coll Med, Dept Mol & Human Genet, Ctr Human Genome, Houston, TX 77030 USA. [Westwater, Caroline] Med Univ S Carolina, Dept Oral Hlth Sci, Charleston, SC 29425 USA. [Bry, Lynn] Brigham & Womens Hosp, Harvard Digest Dis Ctr, Ctr Clin & Translat Metagen, Boston, MA 02115 USA. [Swennes, Alton G.] Baylor Coll Med, Ctr Comparat Med, Houston, TX 77030 USA. [Swennes, Alton G.] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA. [Moore, Sean R.] Cincinnati Childrens Hosp Med Ctr, Ctr Global Child Hlth, Gastroenterol Hepatol & Nutr, Cincinnati, OH 45229 USA. RP Shen, LL (reprint author), Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St,Ste 8020, Houston, TX 77030 USA. EM Lanlan.Shen@bcm.edu FU Sidney Kimmel Foundation; US Department of Agriculture [CRIS 6250-51000-055, CRIS 6250-51000-054]; March of Dimes [1-FY-08-392]; National Institutes of Health [R01HD061916, 1R01DK081557, K02TW008767, 1R21CA137689]; Bill & Melinda Gates Grand Challenges Explorations Grant [OPP1109785] FX This work was supported by grants from the Sidney Kimmel Foundation (to L.S.); US Department of Agriculture (CRIS 6250-51000-055 to L.S. and R.A.W., CRIS 6250-51000-054 to M.H.C.); March of Dimes (1-FY-08-392 to R.A.W.); National Institutes of Health (R01HD061916 to L.B., 1R01DK081557 to R.A.W., K02TW008767 to S.R.M., and 1R21CA137689 to L.S.), and a Bill & Melinda Gates Grand Challenges Explorations Grant (OPP1109785 to S.R.M. and L.S.). We would like to thank Adam Gillum for assistance with the figures. We are grateful for the shared resources provided by the Cellular and Molecular Morphology core at the Texas Medical Center Digestive Disease Center (NIH/NIDDK P30 DK56338), Harvard Digestive Disease Center Gnotobiotic Mouse Core (NIH/NIDDK P30 DK034854), MUSC Gnotobiotic Animal Core (NIH/NIGMS P30GM103331) and Baylor College of Medicine Gnotobiotic Mouse Facility. Fluorescence activated sorting was performed in the Texas Children's Cancer and Hematology Core Flow Laboratory with the expert assistance of Tatiana Goltsova. NR 66 TC 8 Z9 8 U1 5 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1465-6906 EI 1474-760X J9 GENOME BIOL JI Genome Biol. PD SEP 30 PY 2015 VL 16 AR 211 DI 10.1186/s13059-015-0763-5 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CS2CF UT WOS:000361874700001 PM 26420038 ER PT J AU Neill, JD Dubovi, EJ Ridpath, JF AF Neill, John D. Dubovi, Edward J. Ridpath, Julia F. TI Identification of amino acid changes in the envelope glycoproteins of bovine viral diarrhea viruses isolated from alpaca that may be involved in host adaptation SO VETERINARY MICROBIOLOGY LA English DT Article DE Persistent infection; Host adaptation; Genetic change; Phylogenetic analysis; Camelid ID NEW-WORLD CAMELIDS; SWINE-FEVER VIRUS; WHITE-TAILED DEER; E-RNS; PERSISTENT INFECTION; E2; ESTABLISHMENT; PESTIVIRUSES AB Bovine viral diarrhea viruses (BVDV) are most commonly associated with infections of cattle. However, BVDV are often isolated from closely related ruminants with a number of BVDV-1b viruses being isolated from alpacas that were both acutely and persistently infected. The complete nucleotide sequence of the open reading frame of eleven alpaca-adapted BVDV isolates and the region encoding the envelope glycoproteins of an additional three isolates were determined. With the exception of one, all alpaca isolates were >99.2% similar at the nucleotide level. The Hercules isolate was more divergent, with 95.7% sequence identity to the other viruses. Sequence similarity of the 14 viruses indicated they were isolates of a single BVDV strain that had adapted to and were circulating through alpaca herds. Hercules was a more distantly related strain that has been isolated only once in Canada and represented a separate adaptation event that possessed the same adaptive changes. Comparison of amino acid sequences of alpaca and bovine-derived BVDV strains revealed three regions with amino acid sequences unique to all alpaca isolates. The first contained two small in-frame deletions near the N-terminus of the E2 glycoprotein. The second was found near the C-terminus of the E2 protein where four altered amino acids were located within a 30 amino acid domain that participates in E2 homodimerization. The third region contained three variable amino acids in the C-terminus of the E-rns within the amphipathic helix membrane anchor. These changes were found in the polar side of the amphipathic helix and resulted in an increased charge within the polar face. Titration of bovine and alpaca viruses in both bovine and alpaca cells indicated that with increased charge in the amphipathic helix, the ability to infect alpaca cells also increased. Published by Elsevier B.V. C1 [Neill, John D.; Ridpath, Julia F.] ARS, USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. [Dubovi, Edward J.] Cornell Univ, Coll Vet Med, Anim Hlth Diagnost Ctr, Ithaca, NY 14853 USA. RP Neill, JD (reprint author), ARS, USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM john.neill@ars.usda.gov FU Alpaca Research Foundation FX Sincere thanks are given to the Alpaca Research Foundation for the generous funding provided for this project. The authors would like to thank Renae Lesan, Ashley Holmes and Kathy Fulk for excellent technical assistance. Thanks are given to Dr. James Evermann for the 10270 virus isolate and to Dr. Sagar Goyal for the gift of the alpaca fetus BVDV strain 23909. Appreciation is also expressed to Dr. David Alt and Lea Ann Hobbs of the NADC DNA sequencing facility for their help in the sequence analysis of these alpaca viruses and to Dr. Darrell Bayles for his assistance in amino acid comparisons. NR 25 TC 2 Z9 2 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD SEP 30 PY 2015 VL 179 IS 3-4 BP 299 EP 303 DI 10.1016/j.vetmic.2015.06.007 PG 5 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CP9ZR UT WOS:000360254900023 PM 26072370 ER PT J AU Luedtke, BE Bosilevac, JM AF Luedtke, Brandon E. Bosilevac, Joseph M. TI Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE veal; total EHEC; multiplex qPCR; MPN; dPCR ID POLYMERASE-CHAIN-REACTION; CATTLE FECES; VIRULENCE FACTORS; BOVINE FECES; BEEF-CATTLE; DIGITAL PCR; E. COLI; CALVES; O157; STEC AB The increased association of enterohemorrhagic Escherichia coli (EHEC) with veal calves has led the United States Department of Agriculture Food Safety and Inspection Service to report results of veal meat contaminated with the Top 7 serogroups separately from beef cattle. However, detection methods that can also provide concentration for determining the prevalence and abundance of EHEC associated with veal are lacking. Here we compared the ability of qPCR and a molecular based most probable number assay (MPN) to detect and enumerate EHEC from veal hides at the abattoir and the resulting pre-intervention carcasses. In addition, digital PCR (dPCR) was used to analyze select samples. The qPCR assay was able to enumerate total EHEC in 32% of the hide samples with a range of approximately 34 to 91,412 CFUs/100 cm(2) (95% Cl 4-113,460 CFUs/100 cm(2)). Using the MPN assay, total EHEC was enumerable in 48% of the hide samples and ranged from approximately 1 to greater than 17,022 CFUs/100 cm2 (95% Cl 0.4-72,000 CFUs/100 cm(2)). The carcass samples had lower amounts of EHEC with a range of approximately 4-275 CFUs/100 cm(2) (95% Cl 3-953 CFUs/100 cm(2)) from 17% of samples with an enumerable amount of EHEC by qPCR. For the MPN assay, the carcass samples ranged from 0.1 to 1 CFUs/100 cm(2) (95% Cl 0.02-4 CFU5/100 cm(2)) from 29% of the samples. The correlation coefficient between the qPCR and MPN enumeration methods indicated a moderate relation (R-2 = 0.39) for the hide samples while the carcass samples had no relation (R-2 = 0.002), which was likely due to most samples having an amount of total EHEC below the reliable limit of quantification for qPCR. Interestingly, after enrichment, 81% of the hide samples and 94% of the carcass samples had a detectable amount of total EHEC by qPCR. From our analysis, the MPN assay provided a higher percentage of enumerable hide and carcass samples, however determining an appropriate dilution range and the limited throughput offer additional challenges. C1 [Luedtke, Brandon E.; Bosilevac, Joseph M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Bosilevac, JM (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, State Spur 18D, Clay Ctr, NE 68933 USA. EM mick.bosilevac@ars.usda.gov NR 41 TC 3 Z9 3 U1 2 U2 7 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD SEP 29 PY 2015 VL 6 AR 1062 DI 10.3389/fmicb.2015.01062 PG 11 WC Microbiology SC Microbiology GA CU2RW UT WOS:000363371900001 PM 26483780 ER PT J AU Dhar, S Kumar, A Rimando, AM Zhang, X Levenson, AS AF Dhar, Swati Kumar, Avinash Rimando, Agnes M. Zhang, Xu Levenson, Anait S. TI Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR-17 family in prostate cancer SO ONCOTARGET LA English DT Article DE oncomiRs; prostate cancer epigenetics; PTEN; pterostilbene; resveratrol ID MICRORNA EXPRESSION; TUMOR-GROWTH; CELL-LINES; IN-VIVO; MECHANISMS; CLUSTER; PATHWAY; PHARMACOKINETICS; DERIVATIVES; METASTASIS AB In recent years, not only has the role of miRNAs in cancer become increasingly clear but also their utilization as potential biomarkers and therapeutic targets has gained ground. Although the importance of dietary stilbenes such as resveratrol and pterostilbene as anti-cancer agents is well recognized, our understanding of their miRNA-targeting capabilities is still limited. In our previous study, we reported that resveratrol downregulates PTEN-targeting members of the oncogenic miR-17 family, which are overexpressed in prostate cancer. This study investigates the resveratrol and pterostilbene induced miRNA-mediated regulation of PTEN in prostate cancer. Here, we show that both compounds decrease the levels of endogenous as well as exogenously expressed miR-17, miR-20a and miR-106b thereby upregulating their target PTEN. Using functional luciferase reporter assays, we demonstrate that ectopically expressed miR-17, miR-20a and miR-106b directly target PTEN 3'UTR to reduce its expression, an effect rescued upon treatment with resveratrol and pterostilbene. Moreover, while stable lentiviral expression of miR-17/106a significantly decreased PTEN mRNA and protein levels and conferred survival advantage to the cells, resveratrol and more so pterostilbene was able to dramatically suppress these effects. Further, pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation, rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo. Our findings implicate dietary stilbenes as an attractive miRNA-mediated chemopreventive and therapeutic strategy, and circulating miRNAs as potential chemopreventive and predictive biomarkers for clinical development in prostate cancer. C1 [Dhar, Swati; Kumar, Avinash; Levenson, Anait S.] Univ Mississippi, Med Ctr, Canc Inst, Jackson, MS 39216 USA. [Rimando, Agnes M.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS USA. [Zhang, Xu] Univ Mississippi, Med Ctr, Ctr Biostat & Bioinformat, Jackson, MS 39216 USA. [Levenson, Anait S.] Univ Mississippi, Med Ctr, Dept Pathol, Jackson, MS 39216 USA. RP Levenson, AS (reprint author), Univ Mississippi, Med Ctr, Canc Inst, Jackson, MS 39216 USA. EM alevenson@umc.edu FU Department of Defense Prostate Cancer Research Program [W81XWH-13-1-0370] FX This study was supported in part by the Department of Defense Prostate Cancer Research Program under Award W81XWH-13-1-0370 to AS Levenson. Views and opinions of, and endorsements by the author(s) do not reflect those of the US Army of the Department of Defense. NR 56 TC 13 Z9 15 U1 2 U2 7 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD SEP 29 PY 2015 VL 6 IS 29 BP 27214 EP 27226 DI 10.18632/oncotarget.4877 PG 13 WC Oncology; Cell Biology SC Oncology; Cell Biology GA CT9UE UT WOS:000363161300059 PM 26318586 ER PT J AU Nakahara, S O'Donnell, CA Mound, LA AF Nakahara, Steve O'Donnell, Cheryle A. Mound, Laurence A. TI Heliothrips haemorrhoidalis and its relatives, with one new species and one new genus (Thysanoptera: Thripidae) SO ZOOTAXA LA English DT Article DE greenhouse thrips; Heliothrips; Neotropics; new species; new genus AB Heliothrips similis sp.n. is described as a close relative of the greenhouse thrips, Heliothrips haemorrhoidalis. This is the third species recognized here in the Neotropical genus Heliothrips. A pest species described from South Africa as Heliothrips sylvanus is transferred to a new genus, Neoheliothrips gen.n., as Neoheliothrips sylvanus (Faure) comb.n. C1 [Nakahara, Steve; O'Donnell, Cheryle A.] USDA, Systemat Entomol Lab, Beltsville, MD 20705 USA. [Mound, Laurence A.] CSIRO, Australian Natl Insect Collect, Canberra, ACT, Australia. RP Nakahara, S (reprint author), USDA, Systemat Entomol Lab, Beltsville, MD 20705 USA. EM Cheryle.A.ODonnell@aphis.usda.gov; laurence.mound@csiro.au NR 0 TC 1 Z9 1 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD SEP 29 PY 2015 VL 4021 IS 4 BP 578 EP 578 PG 1 WC Zoology SC Zoology GA CS1SH UT WOS:000361847500007 PM 26624155 ER PT J AU Yilmazel, YD Johnston, D Duran, M AF Yilmazel, Yasemin D. Johnston, David Duran, Metin TI Hyperthermophilic hydrogen production from wastewater biosolids by Caldicellulosiruptor bescii SO INTERNATIONAL JOURNAL OF HYDROGEN ENERGY LA English DT Article DE Hyperthermophilic hydrogen production; Biosolids; Caldicellulosiruptor bescii ID THERMOPHILUM DSM 6725; BIOHYDROGEN PRODUCTION; ETHANOL-PRODUCTION; PLANT BIOMASS; FERMENTATION; SLUDGE; PRETREATMENT; YIELD; SACCHAROLYTICUS; DEGRADATION AB Wastewater biosolids are abundant renewable resources that are rich in organic matter and offer a low cost potential feedstock for biohydrogen production. Relevant literature indicates that biosolids conversion rates are relatively low and therefore this option is not considered feasible. This study showed that hyperthermophilic bacteria Caldicellulosiruptor bescii could efficiently utilize biosolids as the sole carbon source and produce hydrogen (H-2) for the first time in the literature. Degradability assessment studies were performed in 60 mL serum bottles at 78 +/- 2 degrees C and the results of this study are very promising indeed. Owing to superior H-2 production characteristics of C. bescii, a H-2 yield of 86 mL per gram dry solids was achieved and this is the highest dark fermentative H-2 yield obtained from biosolids so far. Various chromatographic methods were employed to identify the other major fermentation end products and acetic acid was found as the only major fermentation product in the biosolids reactors. Copyright (C) 2015, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. C1 [Yilmazel, Yasemin D.; Duran, Metin] Villanova Univ, Dept Civil & Environm Engn, Villanova, PA 19085 USA. [Johnston, David] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Yilmazel, YD (reprint author), Penn State Univ, Dept Civil & Environm Engn, University Pk, PA 16802 USA. EM yasemin.yilmazel@villanova.edu FU American Refining and Biochemical, Inc.; Edward A. Daylor chair in Environmental Engineering FX The authors express sincere appreciation to the American Refining and Biochemical, Inc. for sponsoring the study. Partial support for Yasemin Yilmazel was provided by Edward A. Daylor chair in Environmental Engineering. NR 43 TC 2 Z9 2 U1 1 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0360-3199 EI 1879-3487 J9 INT J HYDROGEN ENERG JI Int. J. Hydrog. Energy PD SEP 28 PY 2015 VL 40 IS 36 BP 12177 EP 12186 DI 10.1016/j.ijhydene.2015.06.140 PG 10 WC Chemistry, Physical; Electrochemistry; Energy & Fuels SC Chemistry; Electrochemistry; Energy & Fuels GA CR5VD UT WOS:000361411600014 ER PT J AU Marcolino-Gomes, J Rodrigues, FA Fuganti-Pagliarini, R Nakayama, TJ Reis, RR Farias, JRB Harmon, FG Molinari, HBC Molinari, MDC Nepomuceno, A AF Marcolino-Gomes, Juliana Rodrigues, Fabiana Aparecida Fuganti-Pagliarini, Renata Nakayama, Thiago Jonas Reis, Rafaela Ribeiro Farias, Jose Renato Boucas Harmon, Frank G. Correa Molinari, Hugo Bruno Correa Molinari, Mayla Daiane Nepomuceno, Alexandre TI Transcriptome-Wide Identification of Reference Genes for Expression Analysis of Soybean Responses to Drought Stress along the Day SO PLOS ONE LA English DT Article ID ARABIDOPSIS CIRCADIAN CLOCK; REAL-TIME PCR; OSMOTIC ADJUSTMENT; GLYCINE-MAX; QPCR DATA; NORMALIZATION; PLANTS; RESISTANCE; TOLERANCE; THALIANA AB The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptome-wide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-beta and beta-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. C1 [Marcolino-Gomes, Juliana; Rodrigues, Fabiana Aparecida; Fuganti-Pagliarini, Renata; Nakayama, Thiago Jonas; Reis, Rafaela Ribeiro; Farias, Jose Renato Boucas; Correa Molinari, Mayla Daiane; Nepomuceno, Alexandre] Brazilian Agr Res Corp, Embrapa Soybean, Londrina, Parana, Brazil. [Marcolino-Gomes, Juliana; Reis, Rafaela Ribeiro; Correa Molinari, Mayla Daiane] Univ Estadual Londrina, Dept Biol, Londrina, Parana, Brazil. [Nakayama, Thiago Jonas] Univ Fed Vicosa, Dept Crop Sci, Vicosa, MG, Brazil. [Harmon, Frank G.] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Correa Molinari, Hugo Bruno] Brazilian Agr Res Corp, Embrapa Agroenergy, Brasilia, DF, Brazil. RP Nepomuceno, A (reprint author), Brazilian Agr Res Corp, Embrapa Soybean, Londrina, Parana, Brazil. EM alexandre.nepomuceno@embrapa.br OI Fuganti-Pagliarini, Renata /0000-0001-9282-2826 FU National Council for Scientific and Technological Development (CNPq); Brazilian Federal Agency for the Support and Evaluation of Graduate Education (CAPES) FX This study was supported by the National Council for Scientific and Technological Development (CNPq) and the Brazilian Federal Agency for the Support and Evaluation of Graduate Education (CAPES). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 2 Z9 2 U1 6 U2 34 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 25 PY 2015 VL 10 IS 9 AR e0139051 DI 10.1371/journal.pone.0139051 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS1CR UT WOS:000361800700154 PM 26407065 ER PT J AU Mustafa, T Horton, DR Cooper, WR Swisher, KD Zack, RS Pappu, HR Munyaneza, JE AF Mustafa, Tariq Horton, David R. Cooper, W. Rodney Swisher, Kylie D. Zack, Richard S. Pappu, Hanu R. Munyaneza, Joseph E. TI Use of Electrical Penetration Graph Technology to Examine Transmission of 'Candidatus Liberibacter solanacearum' to Potato by Three Haplotypes of Potato Psyllid (Bactericera cockerelli; Hemiptera: Triozidae) SO PLOS ONE LA English DT Article ID ZEBRA CHIP DISEASE; STYLET PENETRATION; UNITED-STATES; 1ST REPORT; INOCULATION; ACQUISITION; VIRUS; BEHAVIOR; APHIDS; PLANT AB The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a vector of the phloem-limited bacterium 'Candidatus Liberibacter solanacearum' (Lso), the putative causal agent of zebra chip disease of potato. Little is known about how potato psyllid transmits Lso to potato. We used electrical penetration graph (EPG) technology to compare stylet probing behaviors and efficiency of Lso transmission of three haplotypes of potato psyllid (Central, Western, Northwestern). All haplotypes exhibited the full suite of stylet behaviors identified in previous studies with this psyllid, including intercellular penetration and secretion of the stylet pathway, xylem ingestion, and phloem activities, the latter comprising salivation and ingestion. The three haplotypes exhibited similar frequency and duration of probing behaviors, with the exception of salivation into phloem, which was of higher duration by psyllids of the Western haplotype. We manipulated how long psyllids were allowed access to potato ("inoculation access period", or IAP) to examine the relationship between phloem activities and Lso transmission. Between 25 and 30% of psyllids reached and salivated into phloem at an IAP of 1 hr, increasing to almost 80% of psyllids as IAP was increased to 24 h. Probability of Lso-transmission was lower across all IAP levels than probability of phloem salivation, indicating that a percentage of infected psyllids which salivated into the phloem failed to transmit Lso. Logistic regression showed that probability of transmission increased as a function of time spent salivating into the phloem; transmission occurred as quickly as 5 min following onset of salivation. A small percentage of infected psyllids showed extremely long salivation events but nonetheless failed to transmit Lso, for unknown reasons. Information from these studies increases our understanding of Lso transmission by potato psyllid, and demonstrates the value of EPG technology in exploring questions of vector efficiency. C1 [Mustafa, Tariq; Horton, David R.; Cooper, W. Rodney; Swisher, Kylie D.; Munyaneza, Joseph E.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Mustafa, Tariq; Zack, Richard S.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Pappu, Hanu R.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Munyaneza, JE (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Joseph.Munyaneza@ars.usda.gov FU Northwest Potato Research Consortium, Washington State Potato Commission; United States Department of Agriculture, Agricultural Research Service State Cooperative Potato Research Program; United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative [2009-51181-20176] FX Funding: Financial support for this research was partially provided by the Northwest Potato Research Consortium, Washington State Potato Commission; United States Department of Agriculture, Agricultural Research Service State Cooperative Potato Research Program; and United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative (Project #2009-51181-20176). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 46 TC 4 Z9 4 U1 2 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 25 PY 2015 VL 10 IS 9 AR e0138946 DI 10.1371/journal.pone.0138946 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS1CR UT WOS:000361800700124 PM 26407093 ER PT J AU Zhang, TJ Yu, LX Zheng, P Li, YJ Rivera, M Main, D Greene, SL AF Zhang, Tiejun Yu, Long-Xi Zheng, Ping Li, Yajun Rivera, Martha Main, Dorrie Greene, Stephanie L. TI Identification of Loci Associated with Drought Resistance Traits in Heterozygous Autotetraploid Alfalfa (Medicago sativa L.) Using Genome-Wide Association Studies with Genotyping by Sequencing SO PLOS ONE LA English DT Article ID ARABIDOPSIS-THALIANA; ENVIRONMENTAL-STRESS; ENHANCED TOLERANCE; GENETIC DIVERSITY; GERMPLASMS; PROTEIN; FORAGE; YIELD; TRANSCRIPTION; PRODUCTIVITY AB Drought resistance is an important breeding target for enhancing alfalfa productivity in arid and semi-arid regions. Identification of genes involved in drought tolerance will facilitate breeding for improving drought resistance and water use efficiency in alfalfa. Our objective was to use a diversity panel of alfalfa accessions comprised of 198 cultivars and landraces to identify genes involved in drought tolerance. The panel was selected from the USDA-ARS National Plant Germplasm System alfalfa collection and genotyped using genotyping by sequencing. A greenhouse procedure was used for phenotyping two important traits associated with drought tolerance: drought resistance index (DRI) and relative leaf water content (RWC). Marker-trait association identified nineteen and fifteen loci associated with DRI and RWC, respectively. Alignments of target sequences flanking to the resistance loci against the reference genome of M. truncatula revealed multiple chromosomal locations. Markers associated with DRI are located on all chromosomes while markers associated with RWC are located on chromosomes 1, 2, 3, 4, 5, 6 and 7. Co-localizations of significant markers between DRI and RWC were found on chromosomes 3, 5 and 7. Most loci associated with DRI in this work overlap with the reported QTLs associated with biomass under drought in alfalfa. Additional significant markers were targeted to several contigs with unknown chromosomal locations. BLAST search using their flanking sequences revealed homology to several annotated genes with functions in stress tolerance. With further validation, these markers may be used for marker-assisted breeding new alfalfa varieties with drought resistance and enhanced water use efficiency. C1 [Zhang, Tiejun; Yu, Long-Xi; Li, Yajun; Rivera, Martha] ARS, Plant & Germplasm Intro & Testing Res, USDA, Prosser, WA USA. [Zheng, Ping; Main, Dorrie] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Greene, Stephanie L.] ARS, Natl Ctr Genet Resource Preservat, USDA, Ft Collins, CO USA. RP Yu, LX (reprint author), ARS, Plant & Germplasm Intro & Testing Res, USDA, Prosser, WA USA. EM longxi.yu@ars.usda.gov FU USDA-ARS National Program Project [5354-21000-015-00D]; National Alfalfa and Forage Alliance FX Funding: This work was supported by USDA-ARS National Program Project No. 5354-21000-015-00D and by a partial grant from the National Alfalfa and Forage Alliance to L-X Yu. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 4 Z9 4 U1 5 U2 34 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 25 PY 2015 VL 10 IS 9 AR e0138931 DI 10.1371/journal.pone.0138931 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS1CR UT WOS:000361800700120 PM 26406473 ER PT J AU Willett, DS Alborn, HT Duncan, LW Stelinski, LL AF Willett, Denis S. Alborn, Hans T. Duncan, Larry W. Stelinski, Lukasz L. TI Social Networks of Educated Nematodes SO SCIENTIFIC REPORTS LA English DT Article ID ENTOMOPATHOGENIC NEMATODES; DIAPREPES-ABBREVIATUS; BEHAVIORAL PLASTICITY; VOLATILES; ELEGANS; MODELS; ROOTS AB Entomopathogenic nematodes are obligate lethal parasitoids of insect larvae that navigate a chemically complex belowground environment while interacting with their insect hosts, plants, and each other. In this environment, prior exposure to volatile compounds appears to prime nematodes in a compound specific manner, increasing preference for volatiles they previously were exposed to and decreasing attraction to other volatiles. In addition, persistence of volatile exposure influences this response. Longer exposure not only increases preference, but also results in longer retention of that preference. These entomopathogenic nematodes display interspecific social behavioral plasticity; experienced nematodes influence the behavior of different species. This interspecific social behavioral plasticity suggests a mechanism for rapid adaptation of belowground communities to dynamic environments. C1 [Willett, Denis S.; Duncan, Larry W.; Stelinski, Lukasz L.] Univ Florida, Dept Entomol & Nematol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Alborn, Hans T.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Willett, DS (reprint author), Univ Florida, Dept Entomol & Nematol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. EM dwillett@ufl.edu FU University of Florida FX We thank Karen Addison for maintaining nematode cultures and preparing bioassays. Erik Linder, Barry Fleming, Wendy Meyer, and other members of the Stelinski lab contributed valuable assistance, suggestions, and feedback regarding experiments. An internal grant from the University of Florida funded the investigation. NR 24 TC 3 Z9 3 U1 4 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD SEP 25 PY 2015 VL 5 AR 14388 DI 10.1038/srep14388 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS0EX UT WOS:000361731900001 PM 26404058 ER PT J AU Ladinig, A Ashley, C Detmer, SE Wilkinson, JM Lunney, JK Plastow, G Harding, JCS AF Ladinig, Andrea Ashley, Carolyn Detmer, Susan E. Wilkinson, Jamie M. Lunney, Joan K. Plastow, Graham Harding, John C. S. TI Maternal and fetal predictors of fetal viral load and death in third trimester, type 2 porcine reproductive and respiratory syndrome virus infected pregnant gilts SO VETERINARY RESEARCH LA English DT Article ID HOST RESPONSE; TRANSPLACENTAL INFECTION; PRENATAL UNDERNUTRITION; MICROSCOPIC LESIONS; IMPLANTATION SITES; ANTIBODY-RESPONSE; FETUSES; PRRSV; GESTATION; SOWS AB Minimal research has focused on understanding mechanisms underlying porcine reproductive and respiratory syndrome virus (PRRSV) induced reproductive failure. We have completed a large-scale project investigating phenotypic and genotypic predictors of reproductive PRRS severity in which numerous clinical, pathological, immunologic and viral responses were characterized in dams and fetuses. The goal was to determine which phenotypic responses were associated with fetal viral load and death after experimental infection of pregnant gilts with type 2 PRRSV, thereby elucidating mechanisms of reproductive PRRS in third trimester pregnant gilts. The presence of fetal infection and increasing RNA concentration at the maternal-fetal interface were strong predictors of the probability of fetal death, while PRRSV RNA concentration in dam sera and systemic tissues were not associated with the odds of fetal death. Fetal infection and death clustered, indicating that the status of adjacent fetuses is crucial for lateral transmission and fetal outcome. Several systemic immune responses of gilts were associated with fetal outcome and viral load: interferon-a contributed to the probability of fetal death, but absolute numbers of T helper cells in early infection, absolute numbers of myeloid cells over time and interleukin 12 levels appeared protective. These results suggest specific immune responses may either contribute to, or protect against, transplacental virus transmission. The WUR10000125 SNP on chromosome 4, associated with PRRS resilience in nursery pigs, was not associated with reproductive outcome. Whereas past research suggested that fetal death results from events occurring at the maternal-fetal interface, we conclude that viral replication within fetuses and spread of PRRSV to adjacent fetuses are pivotal events in the pathogenesis of reproductive PRRS. C1 [Ladinig, Andrea; Ashley, Carolyn; Harding, John C. S.] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 5B4, Canada. [Ladinig, Andrea] Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Univ Clin Swine, A-1210 Vienna, Austria. [Detmer, Susan E.] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK S7N 5B4, Canada. [Wilkinson, Jamie M.; Plastow, Graham] Univ Alberta, Fac Agr Life & Environm Sci, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. [Lunney, Joan K.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Ladinig, A (reprint author), Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, 52 Campus Dr, Saskatoon, SK S7N 5B4, Canada. EM andrea.ladinig@vetmeduni.ac.at OI Ladinig, Andrea/0000-0001-5037-7269 FU Genome Canada; Genome Prairie [2209-F] FX The authors wish to acknowledge the numerous technicians and students from the Western College of Veterinary Medicine, Vaccine and Infectious Disease Organization, Prairie Diagnostic Services, Inc. and University of Alberta who assisted with this project. We offer special thanks to those taking leadership roles including Stewart Walker, Don Wilson, Tuanjie Chang, Kyle Clarke, Linda Ye and Lois Ridgeway, and to Ian Dohoo and Henrik Stryhn for their guidance with statistical analyses. Illustrations were prepared by Juliane Deubner, Medical Illustrator, at the Western College of Veterinary Medicine. The Taqman assay for the WUR10000125 SNP was developed with Urmila Basu at the University of Alberta. Pregnant gilts were provided and bred by Fast Genetics Inc., Spiritwood with management support of Dawn Friesen, Connie Heisler, Donell Wingerter and Benny Mote. Funding for the project was generously provided by grants from Genome Canada and Genome Prairie (grant number 2209-F), with administrative support from Genome Alberta. NR 41 TC 8 Z9 8 U1 1 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD SEP 25 PY 2015 VL 46 AR 107 DI 10.1186/s13567-015-0251-7 PG 15 WC Veterinary Sciences SC Veterinary Sciences GA CS0XL UT WOS:000361785400001 PM 26407558 ER PT J AU Stetson, SJ Osborne, SL AF Stetson, Sarah J. Osborne, Shannon L. TI Further Modification of Pressure-Calcimeter Method for Soil Inorganic Carbon Analysis SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE pressure calcimeter method; soil analysis; Inorganic carbon AB Inorganic carbon (IC) in soil is important when considering carbon (C) fluxes and processes in the environment that involve C. Here data are presented for measurement of IC in soils using the pressure-calcimeter method of Sherrod et al. (2002) with modifications to decrease analysis time while preserving method accuracy. Inorganic C measured using a syringe to inject acid vs. the vial delivery method of the original method were not significantly different at the 95 percent confidence level when using 100-ml bottles for the analysis (R-2=0.9946). When using 20-ml bottles, bias was introduced in the measurement when using a syringe for acid delivery, likely due to small losses of pressure from the bottle during acid delivery. There was no significant difference at the 95 percent confidence level in IC measured on unsieved finely ground and coarsely ground soil. When quantifying IC in soils using the modified pressure calcimeter method with a 100-ml bottle, a syringe can be used to inject acid into the capped bottle rather than dropping a vial into the bottle and then capping it. This modification results in a modest reduction in cost and significant reduction in time required for analysis and cleanup. C1 [Stetson, Sarah J.; Osborne, Shannon L.] ARS, USDA, NCARL, Brookings, SD 57006 USA. RP Osborne, SL (reprint author), ARS, USDA, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM Shannon.Osborne@ars.usda.gov OI Stetson, Sarah/0000-0002-4930-4748 NR 4 TC 0 Z9 0 U1 2 U2 7 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0010-3624 EI 1532-2416 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD SEP 25 PY 2015 VL 46 IS 17 BP 2162 EP 2167 DI 10.1080/00103624.2015.1069316 PG 6 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA CR5JT UT WOS:000361378200005 ER PT J AU Root, JJ Shriner, SA Ellis, JW VanDalen, KK Sullivan, HJ Franklin, AB AF Root, J. Jeffrey Shriner, Susan A. Ellis, Jeremy W. VanDalen, Kaci K. Sullivan, Heather J. Franklin, Alan B. TI When fur and feather occur together: interclass transmission of avian influenza A virus from mammals to birds through common resources SO SCIENTIFIC REPORTS LA English DT Article ID ENVIRONMENTAL TRANSMISSION; SPREAD; INFECTION; POULTRY AB The potential role of wild mammals in avian influenza A virus (IAV) transmission cycles has received some attention in recent years and cases where birds have transmitted IAV to mammals have been documented. However, the contrasting cycle, wherein a mammal could transmit an avian IAV to birds, has been largely overlooked. We experimentally tested the abilities of two mammalian species to transmit avian IAV to mallards (Anas platyrhynchos) in simulated natural environments. Results suggested that striped skunks (Mephitis mephitis) can successfully transmit avian IAV to mallards through indirect contact with shared resources, as transmission was noted in 1 of 4 of the mallards tested. Cottontail rabbits (Sylvilagus sp.) exhibited a similar pattern, as one of five cottontail rabbits successfully transmitted IAV to a mallard, likely through environmental contamination. For each mammalian species tested, the mallards that became infected were those paired with the individual mammals with the lowest shedding levels but were anecdotally observed to be the most active animals. Mammals associated with and around poultry rearing facilities should be taken into consideration in biosecurity plans. C1 [Root, J. Jeffrey; Shriner, Susan A.; Ellis, Jeremy W.; VanDalen, Kaci K.; Sullivan, Heather J.; Franklin, Alan B.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80526 USA. RP Root, JJ (reprint author), USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80526 USA. EM Jeff.root@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182; Ellis, Jeremy/0000-0003-4512-0906 NR 25 TC 1 Z9 1 U1 1 U2 8 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD SEP 24 PY 2015 VL 5 AR 14354 DI 10.1038/srep14354 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR9EH UT WOS:000361656100001 PM 26400374 ER PT J AU Schuster, GA Taylor, CA Adams, SB AF Schuster, Guenter A. Taylor, Christopher A. Adams, Susan B. TI Procambarus (Girardiella) holifieldi, a new species of crayfish (Decapoda: Cambaridae) from Alabama with a revision of the Hagenianus Group in the subgenus Girardiella SO ZOOTAXA LA English DT Article DE Procambarus; Girardiella; Hagenianus Group; Gracilis Group; new species; crayfish ID GENUS PROCAMBARUS; TEXAS AB Procambarus (Girardiella) holifieldi, new species, is a primary burrowing crayfish from a low-lying field in Perry County, Alabama. It belongs to the Hagenianus Group in the subgenus Girardiella. The new species is morphologically most similar to Procambarus (Girardiella) barbiger. They differ in the size and shape of the caudal processes. Procambarus barbiger has a beard along the mesial margin of the palm of the chela, while the new species lacks the beard. In addition to the description of the new species, the Hagenianus Group is reviewed and new synonymies are provided. We demonstrate that a cephalic process is indeed present in the Hagenianus Group. C1 [Taylor, Christopher A.] Univ Illinois, Prairie Res Inst, Illinois Nat Hist Survey, Champaign, IL 61820 USA. [Adams, Susan B.] US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. RP Schuster, GA (reprint author), 224 Primrose Circle, Richmond, KY 40475 USA. EM guenter.schuster@eku.edu; ctaylor@inhs.illinois.edu; sadams01@fs.fed.us NR 22 TC 1 Z9 1 U1 0 U2 3 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD SEP 24 PY 2015 VL 4021 IS 1 BP 1 EP 32 PG 32 WC Zoology SC Zoology GA CS1RJ UT WOS:000361845100001 PM 26624118 ER PT J AU Liu, SX Vallejo, RL Palti, Y Gao, GT Marancik, DP Hernandez, AG Wiens, GD AF Liu, Sixin Vallejo, Roger L. Palti, Yniv Gao, Guangtu Marancik, David P. Hernandez, Alvaro G. Wiens, Gregory D. TI Identification of single nucleotide polymorphism markers associated with bacterial cold water disease resistance and spleen size in rainbow trout SO FRONTIERS IN GENETICS LA English DT Article ID FLAVOBACTERIUM-PSYCHROPHILUM; ONCORHYNCHUS-MYKISS; RAD MARKERS; MAP; SURVIVAL; FISH; QTL AB Bacterial cold water disease (BCWD) is one of the frequent causes of elevated mortality in salmonid aquaculture. Previously, we identified and validated microsatellites on chromosome Omy19 associated with QTL (quantitative trait loci) for BCWD resistance and spleen size in rainbow trout. Recently, SNPs (single nucleotide polymorphism) have become the markers of choice for genetic analyses in rainbow trout as they are highly abundant, cost-effective and are amenable for high throughput genotyping. The objective of this study was to identify SNP markers associated with BCWD resistance and spleen size using both genome-wide association studies (GWAS) and linkage-based QTL mapping approaches. A total of 298 offspring from the two half-sib families used in our previous study to validate the significant BCWD QTL on chromosome Omy19 were genotyped with RAD-seq (restriction-site-associated DNA sequencing), and 7,849 informative SNPs were identified. Based on GWAS, 18 SNPs associated with BCWD resistance and 20 SNPs associated with spleen size were identified. Linkage-based QTL mapping revealed three significant QTL for BCWD resistance. In addition to the previously validated dam-derived QTL on chromosome Omy19, two significant BCWD QTL derived from the sires were identified on chromosomes Omy8 and Omy25, respectively. A sire-derived significant QTL for spleen size on chromosome Omy2 was detected. The SNP markers reported in this study will facilitate fine mapping to identify positional candidate genes for BCWD resistance in rainbow trout. C1 [Liu, Sixin; Vallejo, Roger L.; Palti, Yniv; Gao, Guangtu; Marancik, David P.; Wiens, Gregory D.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. [Hernandez, Alvaro G.] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. RP Liu, SX (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM sixin.liu@ars.usda.gov NR 25 TC 4 Z9 4 U1 5 U2 20 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-8021 J9 FRONT GENET JI Front. Genet. PD SEP 24 PY 2015 VL 6 AR 298 DI 10.3389/fgene.2015.00298 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA CZ2CN UT WOS:000366912700001 PM 26442114 ER PT J AU Costa-Hurtado, M Afonso, CL Miller, PJ Shepherd, E Cha, RM Smith, D Spackman, E Kapczynski, DR Suarez, DL Swayne, DE Pantin-Jackwood, MJ AF Costa-Hurtado, Mar Afonso, Claudio L. Miller, Patti J. Shepherd, Eric Cha, Ra Mi Smith, Diane Spackman, Erica Kapczynski, Darrell R. Suarez, David L. Swayne, David E. Pantin-Jackwood, Mary J. TI Previous infection with virulent strains of Newcastle disease virus reduces highly pathogenic avian influenza virus replication, disease, and mortality in chickens SO VETERINARY RESEARCH LA English DT Article ID POULTRY; H9N2; COINFECTION; VACCINATION; DUCKS; INTERFERENCE; PATHOBIOLOGY; VACCINES; ASSAY; EGGS AB Highly pathogenic avian influenza virus (HPAIV) and Newcastle disease virus (NDV) are two of the most important viruses affecting poultry worldwide and produce co-infections especially in areas of the world where both viruses are endemic; but little is known about the interactions between these two viruses. The objective of this study was to determine if co-infection with NDV affects HPAIV replication in chickens. Only infections with virulent NDV strains (mesogenic Pigeon/1984 or velogenic CA/2002), and not a lentogenic NDV strain (LaSota), interfered with the replication of HPAIV A/chicken/Queretaro/14588-19/95 (H5N2) when the H5N2 was given at a high dose (10(6.9) EID50) two days after the NDV inoculation, but despite this interference, mortality was still observed. However, chickens infected with the less virulent mesogenic NDV Pigeon/1984 strain three days prior to being infected with a lower dose (10(5.3-5.5) EID50) of the same or a different HPAIV, A/chicken/Jalisco/CPA-12283-12/2012 (H7N3), had reduced HPAIV replication and increased survival rates. In conclusion, previous infection of chickens with virulent NDV strains can reduce HPAIV replication, and consequently disease and mortality. This interference depends on the titer of the viruses used, the virulence of the NDV, and the timing of the infections. The information obtained from these studies helps to understand the possible interactions and outcomes of infection (disease and virus shedding) when HPAIV and NDV co-infect chickens in the field. C1 [Costa-Hurtado, Mar; Afonso, Claudio L.; Miller, Patti J.; Shepherd, Eric; Cha, Ra Mi; Smith, Diane; Spackman, Erica; Kapczynski, Darrell R.; Suarez, David L.; Swayne, David E.; Pantin-Jackwood, Mary J.] USDA ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr, Athens, GA 30613 USA. RP Pantin-Jackwood, MJ (reprint author), USDA ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr, Athens, GA 30613 USA. EM Mary.Pantin-Jackwood@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU U.S Department of Agriculture, ARS CRIS [6612-32000-063-00D]; National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA [AAI12004-001-00001] FX The authors appreciate the assistance provided by Aniko Zsak, Tim Olivier, Dawn Williams-Coplin, Ronald Graham, and Roger Brock in conducting these studies. This research was supported by the U.S Department of Agriculture, ARS CRIS Project 6612-32000-063-00D and with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA no. AAI12004-001-00001. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 39 TC 1 Z9 1 U1 1 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD SEP 23 PY 2015 VL 46 AR 97 DI 10.1186/s13567-015-0237-5 PG 15 WC Veterinary Sciences SC Veterinary Sciences GA CR8GV UT WOS:000361590000001 PM 26394750 ER PT J AU Gatziolis, D Lienard, JF Vogs, A Strigul, NS AF Gatziolis, Demetrios Lienard, Jean F. Vogs, Andre Strigul, Nikolay S. TI 3D Tree Dimensionality Assessment Using Photogrammetry and Small Unmanned Aerial Vehicles SO PLOS ONE LA English DT Article ID VISUAL LOCATION RECOGNITION; CROWN ASYMMETRY; COMPUTER VISION; DIGITAL IMAGERY; AIRBORNE LIDAR; COMPETITION; FORESTS; HEIGHT; GROWTH; DISTURBANCE AB Detailed, precise, three-dimensional (3D) representations of individual trees are a prerequisite for an accurate assessment of tree competition, growth, and morphological plasticity. Until recently, our ability to measure the dimensionality, spatial arrangement, shape of trees, and shape of tree components with precision has been constrained by technological and logistical limitations and cost. Traditional methods of forest biometrics provide only partial measurements and are labor intensive. Active remote technologies such as LiDAR operated from airborne platforms provide only partial crown reconstructions. The use of terrestrial LiDAR is laborious, has portability limitations and high cost. In this work we capitalized on recent improvements in the capabilities and availability of small unmanned aerial vehicles (UAVs), light and inexpensive cameras, and developed an affordable method for obtaining precise and comprehensive 3D models of trees and small groups of trees. The method employs slow-moving UAVs that acquire images along predefined trajectories near and around targeted trees, and computer vision-based approaches that process the images to obtain detailed tree reconstructions. After we confirmed the potential of the methodology via simulation we evaluated several UAV platforms, strategies for image acquisition, and image processing algorithms. We present an original, step-by-step workflow which utilizes open source programs and original software. We anticipate that future development and applications of our method will improve our understanding of forest self-organization emerging from the competition among trees, and will lead to a refined generation of individual-tree-based forest models. C1 [Gatziolis, Demetrios] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR USA. [Lienard, Jean F.; Vogs, Andre; Strigul, Nikolay S.] Washington State Univ Vancouver, Dept Math, Vancouver, WA 98686 USA. [Vogs, Andre] Intel Inc, Hillsboro, OR USA. RP Strigul, NS (reprint author), Washington State Univ Vancouver, Dept Math, Vancouver, WA 98686 USA. EM nick.strigul@vancouver.wsu.edu RI Strigul, Nick/C-3987-2016 FU Simons Foundation [283770]; Washington State University New Faculty SEED grant FX Funding: This work was partially supported by a grant from the Simons Foundation, (www.simonsfoundation.org) (#283770 to N.S.), and a Washington State University New Faculty SEED grant, (http://faculty.wsu.edu/career/seed-grants/) (to NS). The funders and any other individuals employed had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 66 TC 7 Z9 8 U1 8 U2 42 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 22 PY 2015 VL 10 IS 9 AR e0137765 DI 10.1371/journal.pone.0137765 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS0ZW UT WOS:000361792100018 PM 26393926 ER PT J AU Sun, QR Sun, HY Bell, RL Li, LG Zhou, GF Xin, L Wei, Z AF Sun, Qingrong Sun, Hongyan Bell, Richard L. Li, Linguang Zhou, Guangfang Xin, Li Wei, Zhang TI Field performance of vegetative form traits of neopolyploids produced by in vitro colchicine treatment in Pyrus communis SO SCIENTIA HORTICULTURAE LA English DT Article DE Neopolyploid; Vegetative traits; Variation; Phenotypic abnormality; Stunted growth; Pyrus communis ID TETRAPLOID PLANTS; INDUCTION; POLYPLOIDIZATION; AUTOTETRAPLOIDS; AUTOPOLYPLOIDY; REGENERATION; MECHANISMS; CULTIVAR AB The induction of polyploidy to improve agronomically and economically useful characters is a process commonly used in crop plants. Neopolyploids were obtained from the diploid pear cultivar 'Fertility' by in vitro colchicine treatment. Different clones of polyploids were rooted to be transferred to the greenhouse and then to the net house, a survey orchard was established. The morphological characteristics and the performance of 3 tetraploid clones, 2 triploid clones, 1 mixploid clone and a diploid control were investigated under greenhouse and field conditions. Results revealed that plants of different neopolyploids showed markedly different phenotypic traits. Compared to the diploid control, three out of four polyploid clones plants had significantly thicker leaves and a shorter petiole length. The significance of the difference in leaf index (ratio of leaf lamina length to leaf lamina width) between diploid and polyploids depended on the individual clone of the neopolyploid plants. In the greenhouse and the field, the triploid clone '3x- 1' and the tetraploid clones '4x- 1' and '4x- 2' had significantly decreased leaf index, and the triploid '3x- 2' had significantly increased leaf index. However, the tetraploid clone '4x- 4' and the mixploid clone 'Mix -1" showed phenotypic abnormalities, such as stunted growth and leaf malformation. The ex vivo grafted shoots of the pentaploid '5x- 1' and '5x- 2' were very difficult to maintain or had a very short survival period. The grafted plants of triploid '3x- 2' showed a compact habit. Most neopolyploid plants had significantly shorter internodes and all had reduced plant height compared to the height of their parent diploid plants. (C) 2015 Elsevier B.V. All rights reserved. C1 [Sun, Qingrong; Sun, Hongyan; Li, Linguang; Zhou, Guangfang; Xin, Li] Shandong Inst Pomol, Tai An 27100, Shandong, Peoples R China. [Bell, Richard L.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Wei, Zhang] Shandong Yaoxiang Forestry Farm, Tai An 27103, Shandong, Peoples R China. RP Sun, QR (reprint author), Shandong Inst Pomol, Tai An 27100, Shandong, Peoples R China. EM qingrong_sun@yahoo.com; sdipss@163.com FU Improved Variety Program of Shandong Province of China [(2014)96] FX This research was supported by Improved Variety Program of Shandong Province of China [(2014)96]. NR 43 TC 0 Z9 0 U1 6 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD SEP 22 PY 2015 VL 193 BP 182 EP 187 DI 10.1016/j.scienta.2015.06.047 PG 6 WC Horticulture SC Agriculture GA CS5RS UT WOS:000362136300024 ER PT J AU Glenn, DM Gasic, K AF Glenn, D. Michael Gasic, Ksenija TI Influence of within year treatments and between year environmental differences on peach leaf ash and carbon isotopic discrimination responses SO SCIENTIA HORTICULTURAE LA English DT Article DE Prunus persica; Irrigation; Fertigation; Vegetation free area; Water use efficiency ID WATER-USE EFFICIENCY; MINERAL-CONTENT; GRAIN-YIELD; WHEAT; REGIMES; APPLE AB Plant ash content is related to water use efficiency (WUE) (dry matter accumulation per unit of transpiration) and Delta C-13 in a range of C-3 species. In breeding programs, the genetic X environment interaction is measured with multiple locations and multiple years of evaluation but the genetic X environment interaction of ash or Delta C-13 has not been studied. The purpose of this study was to assess how within year cultural variation and yearly environmental variation affect peach leaf ash and carbon discrimination as measures of water use efficiency using cultural practices evaluated over a 13 year period of time. 'Loring' peach (Prunus persica) on seedling 'Halford' rootstock were planted in 1983 in a 4.5 m x 6 m spacing in square vegetation-free-areas (VFA) around each tree (0.36 and 13.0 m(2)). Three irrigation treatments (none, drip irrigated during stage 3 of fruit development, and fertigation during stage 3 of fruit development) were imposed on each VFA. Leaf ash and carbon isotope discrimination (Delta) were measured in leaf tissue collected prior to harvest. Analysis of variance indicated that there were no differences in VFA or irrigation for either ash or Delta C-13, however there were yearly interactions. Delta C-13 was significantly related to the growing season pan evaporation but the slope was low indicating a minimal response while leaf ash was not significantly related. In contrast, leaf ash was significantly related to the growing season rainfall amount while Delta C-13 was unrelated. Both leaf ash and Delta C-13 were unrelated to the growing season water deficit. The coefficient of variation for leaf ash and Delta C-13 are 0.16 and 0.04, respectively, over a 13 year sampling period. These data suggest that breeding programs screening for increased water use efficiency using leaf ash or Delta C-13 can anticipate a minimal amount of within location variation and thus place more emphasis on environmental or between-location variation. Published by Elsevier B.V. C1 [Glenn, D. Michael] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Gasic, Ksenija] Clemson Univ, Dept Agr & Environm Sci, Clemson, SC 29634 USA. RP Glenn, DM (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov NR 16 TC 0 Z9 0 U1 5 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD SEP 22 PY 2015 VL 193 BP 258 EP 260 DI 10.1016/j.scienta.2015.07.028 PG 3 WC Horticulture SC Agriculture GA CS5RS UT WOS:000362136300034 ER PT J AU Yi, X Gu, HQ Gao, QQ Liu, ZL Bao, J AF Yi, Xia Gu, Hanqi Gao, Qiuqiang Liu, Z. Lewis Bao, Jie TI Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Zymomonas mobilis ZM4; Lignocellulose pretreatment; Phenolic aldehyde inhibitors; Stress tolerance; DNA microarray; Recombinants ID SACCHAROMYCES-CEREVISIAE; DEGRADATION-PRODUCTS; FERMENTATION; ETHANOL; YEAST; EXPRESSION; CONVERSION; REDUCTION; BACTERIA; BIOMASS AB Background: Phenolic aldehydes generated from lignocellulose pretreatment exhibited severe toxic inhibitions on microbial growth and fermentation. Numerous tolerance studies against furfural, 5-hydroxymethyl-2-furaldehyde (HMF), acetate, and ethanol were reported, but studies on inhibition of phenolic aldehyde inhibitors are rare. For ethanologenic strains, Zymomonas mobilis ZM4 is high in ethanol productivity and genetic manipulation feasibility, but sensitive to phenolic aldehyde inhibitors. Molecular mechanisms of tolerance for Z. mobilis toward phenolic aldehydes are not known. Results: We took the first insight into genomic response of Z. mobilis ZM4 to the phenolic aldehyde inhibitors derived from lignocellulose pretreatment. The results suggest that the toxicity to cells is caused by the functional group of phenolic aldehyde, similar to furfural and HMF, rather than aromatic groups or phenolic hydroxyl groups. Transcriptome response against 4-hydroxybenzaldehyde, syringaldehyde, and vanillin, representing phenolic groups H, S, and G, respectively, was investigated. The atlas of the important genes responsible for significantly enhanced and repressed genes at the genomic level was illustrated. 272 genes with twofold greater expressions than non-treated controls and 36 gene clusters in response to challenges of these phenolic aldehydes were identified. Several reductases encoded by ZMO1116, ZMO1696, and ZMO1885 were found to play the key roles in reducing phenolic aldehydes into the corresponding phenolic alcohols. Reduction of phenolic aldehydes by overexpression of ZMO1116, ZMO1696, and ZMO1885 in Z. mobilis ZM4 resulted in the increased inhibitor conversion and ethanol productivity, especially for 4-hydroxybenzaldehyde and vanillin. Several transporter genes such as ZMO0282, ZMO0283, ZMO0798, ZMO0799, and ZMO0800 was also displayed significantly increased expressions against the phenolic aldehydes. Conclusions: The genes encoding reductases are with potentials on phenolic aldehydes-tolerant genes contributing to the reduction of phenolic aldehydes into the corresponding phenolic alcohols forms for Z. mobilis ZM4. Overexpression of the key genes improved the conversion ratio and ethanol productivity of 4-hydroxybenzaldehyde and vanillin with high toxicity. New knowledge obtained from this research aids understanding the mechanisms of bacterial tolerance and the development of the next-generation biocatalysts for advanced biofuels production. C1 [Yi, Xia; Gu, Hanqi; Gao, Qiuqiang; Bao, Jie] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China. [Liu, Z. Lewis] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Liu, ZL (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 North Univ St, Peoria, IL 61604 USA. EM zlewis.liu@ars.usda.gov; jbao@ecust.edu.cn FU National Basic Research Program of China [2011CB707406]; Natural Science Foundation of China [31300070]; National High-Tech Program of China [2012AA022301/2014AA021901] FX This research was supported by the National Basic Research Program of China (2011CB707406), the Natural Science Foundation of China (31300070), and the National High-Tech Program of China (2012AA022301/2014AA021901). NR 31 TC 6 Z9 7 U1 7 U2 31 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD SEP 22 PY 2015 VL 8 AR 153 DI 10.1186/s13068-015-0333-9 PG 15 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA CR8FW UT WOS:000361587500002 PM 26396591 ER PT J AU Baligar, VC Sicher, RC Elson, MK He, Z Fageria, NK De Souza, JO Almeida, AA Ahnert, D AF Baligar, V. C. Sicher, R. C. Elson, M. K. He, Z. Fageria, N. K. De Souza Junior, J. O. Almeida, A. -A. Ahnert, D. TI Iron Sources Effects on Growth, Physiological Parameters and Nutrition of Cacao SO JOURNAL OF PLANT NUTRITION LA English DT Article DE cacao; iron ID CHLOROPHYLL METER; CALCAREOUS SOILS; CROP PRODUCTION; DEFICIENCY; PLANTS; LEAVES; RESPONSES; MICRONUTRIENTS; CAROTENOIDS; EFFICIENCY AB Productivity and sustainability of cacao (Theobroma cacao L.) in tropical soils are affected by levels of iron. Information is lacking on the cacao response to various sources of iron (Fe). A greenhouse experiment was conducted to evaluate the effects of five iron sources iron sulfate heptahydrate, ferric ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid), ferric diethylenetriaminepentaacetic acid, ferric ethylenediaminetetraacetic acid, fiesta herbicide (FeSO4 center dot 7H(2)O, FeEDDHA, FeDTPA, FeEDTA,) at 10mg Fe kg(-1) soil on growth, photosynthesis, content of photosynthetic pigments and starch and macro- and micronutrient nutrition of cacao. The various iron sources had significant effects on shoot and root dry biomass accumulation, leaf chlorophyll a and b content, carotenoid levels, SPAD index and P-N. These parameters were significantly correlated with concentration, uptake, influx, and transport and use efficiency of Fe. In cacao net photosynthesis, stomatal conductance, internal carbon dioxide (CO2)(,) and transpiration in leaf level responded differently to the sources of Fe. Invariably, macro and micronutrient uptake, influx, transport, and use efficiency showed differential responses to sources of iron but significant effects were only observed for copper (Cu), Fe, manganese (Mn), and zinc (Zn). Overall, FeDTPA, FeEDTA and FeHEDTA could be the best sources of Fe in improving, growth, photosynthesis and macro and micro nutrition of cacao. C1 [Baligar, V. C.; Sicher, R. C.; Elson, M. K.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [He, Z.] Univ Florida, IRREC, Ft Pierce, FL USA. [De Souza Junior, J. O.; Almeida, A. -A.; Ahnert, D.] State Univ Santa Cruz UESC, Ilheus, Brazil. RP Baligar, VC (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM VC.Baligar@ars.usda.gov RI He, Zhenli/R-1494-2016 OI He, Zhenli/0000-0001-7761-2070 NR 55 TC 0 Z9 0 U1 2 U2 29 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0190-4167 EI 1532-4087 J9 J PLANT NUTR JI J. Plant Nutr. PD SEP 19 PY 2015 VL 38 IS 11 BP 1787 EP 1802 DI 10.1080/01904167.2015.1061546 PG 16 WC Plant Sciences SC Plant Sciences GA CR2VJ UT WOS:000361188500010 ER PT J AU Camhi, SM Crouter, SE Hayman, LL Must, A Lichtenstein, AH AF Camhi, Sarah M. Crouter, Scott E. Hayman, Laura L. Must, Aviva Lichtenstein, Alice H. TI Lifestyle Behaviors in Metabolically Healthy and Unhealthy Overweight and Obese Women: A Preliminary Study SO PLOS ONE LA English DT Article ID INTENSITY PHYSICAL-ACTIVITY; POSTMENOPAUSAL WOMEN; 2-REGRESSION MODEL; PLASMA-GLUCOSE; NORMAL-WEIGHT; RISK-FACTORS; ADULTS; ACCELEROMETER; PHENOTYPES; VALIDITY AB Background Few studies have examined dietary data or objective measures of physical activity (PA) and sedentary behavior among metabolically healthy overweight/obese (MHO) and metabolically unhealthy overweight/obese (MUO). Thus, the purpose is to determine whether PA, sedentary behavior and/or diet differ between MHO and MUO in a sample of young women. Methods Forty-six overweight/obese (BMI >= 25 kg/m(2)) African American and Caucasian women 1935 years were classified by cardiometabolic risk factors, including elevated blood pressure, triglyceride, glucose and C-reactive protein, low high density lipoprotein, and insulin resistance (MUO >= 2; MHO, < 2). Time (mins/day) in light, moderate, vigorous PA, and sedentary behavior were estimated using an accelerometer (>= 3 days; >= 8 hrs wear time). Questionnaires were used to quantify sitting time, TV/computer use and usual daily activity. The Block Food Frequency Questionnaire assessed dietary food intake. Differences between MHO and MUO for lifestyle behaviors were tested with linear regression (continuous data) or logistic regression (categorical data) after adjusting for age, race, BMI, smoking and accelerometer wear and/or total kilocalories, as appropriate. Results Women were 26.7+/-4.7 years, with a mean BMI of 31.1+/-3.7 kg/m(2), and 61% were African American. Compared to MUO (n = 9), MHO (n = 37; 80%) spent less mins/day in sedentary behavior (difference: -58.1+/-25.5, p = 0.02), more mins/day in light PA (difference: 38.2+/-16.1, p = 0.02), and had higher daily METs (difference: 0.21+/-0.09, p = 0.03). MHO had higher fiber intakes (g/day of total fiber, soluble fiber, fruit/vegetable fiber, bean fiber) and daily servings of vegetables; but lower daily dairy servings, saturated fat, monounsaturated fat and trans fats (g/day) compared to MUO. Conclusion Compared to MUO, MHO young women demonstrate healthier lifestyle habits with less sedentary behavior, more time in light PA, and healthier dietary quality for fat type and fiber. Future studies are needed to replicate findings with larger samples that include men and women of diverse race/ethnic groups. C1 [Camhi, Sarah M.] Univ Massachusetts, Exercise & Hlth Sci Dept, Boston, MA 02125 USA. [Crouter, Scott E.] Univ Tennessee, Dept Kinesiol Recreat & Sport Studies, Knoxville, TN USA. [Hayman, Laura L.] Univ Massachusetts, Off Vice Provost Res, Boston, MA 02125 USA. [Must, Aviva] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. [Lichtenstein, Alice H.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Camhi, SM (reprint author), Univ Massachusetts, Exercise & Hlth Sci Dept, Harbor Campus, Boston, MA 02125 USA. EM sarah.camhi@umb.edu RI Dey, Kamalesh/E-6568-2017 FU University of Massachusetts Boston Proposal Development Grant; Boston Nutrition Obesity Research Center [DK46200] FX Funding support was provided by the University of Massachusetts Boston Proposal Development Grant (no grant number available) and the Boston Nutrition Obesity Research Center DK46200. NR 42 TC 3 Z9 3 U1 4 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 18 PY 2015 VL 10 IS 9 AR e0138548 DI 10.1371/journal.pone.0138548 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS0ZE UT WOS:000361790200124 PM 26383251 ER PT J AU Tako, E Reed, S Anandaraman, A Beebe, SE Hart, JJ Glahn, RP AF Tako, Elad Reed, Spenser Anandaraman, Amrutha Beebe, Steve E. Hart, Jonathan J. Glahn, Raymond P. TI Studies of Cream Seeded Carioca Beans (Phaseolus vulgaris L.) from a Rwandan Efficacy Trial: In Vitro and In Vivo Screening Tools Reflect Human Studies and Predict Beneficial Results from Iron Biofortified Beans SO PLOS ONE LA English DT Article ID DIETARY POLYPHENOLIC COMPOUNDS; CELL-CULTURE MODEL; STAPLE FOOD CROPS; GALLUS-GALLUS; COMMON BEANS; BIOAVAILABLE IRON; ASCORBIC-ACID; YOUNG-WOMEN; ABSORPTION; DEFICIENCY AB Iron (Fe) deficiency is a highly prevalent micronutrient insufficiency predominantly caused by a lack of bioavailable Fe from the diet. The consumption of beans as a major food crop in some populations suffering from Fe deficiency is relatively high. Therefore, our objective was to determine whether a biofortified variety of cream seeded carioca bean (Phaseolus vulgaris L.) could provide more bioavailable-Fe than a standard variety using in-vivo (broiler chicken, Gallus gallus) and in-vitro (Caco-2 cell) models. Studies were conducted under conditions designed to mimic the actual human feeding protocol. Two carioca-beans, a standard (G4825; 58 mu g Fe/g) and a biofortified (SMC; 106 mu g Fe/g), were utilized. Diets were formulated to meet the nutrient requirements of Gallus gallus except for Fe (33.7 and 48.7 mu g Fe/g, standard and biofortified diets, respectively). In-vitro observations indicated that more bioavailable- Fe was present in the biofortified beans and diet (P<0.05). In-vivo, improvements in Fe-status were observed in the biofortified bean treatment, as indicated by the increased total-body-Hemoglobin-Fe, and hepatic Fe-concentration (P<0.05). Also, DMT-1 mRNA-expression was increased in the standard bean treatment (P< 0.05), indicating an upregulation of absorption to compensate for less bioavailable-Fe. These results demonstrate that the biofortified beans provided more bioavailable Fe; however, the in vitro results revealed that ferritin formation values were relatively low. Such observations are indicative of the presence of high levels of polyphenols and phytate that inhibit Fe absorption. Indeed, we identified higher levels of phytate and quercetin 3-glucoside in the Fe biofortified bean variety. Our results indicate that the biofortified bean line was able to moderately improve Fe-status, and that concurrent increase in the concentration of phytate and polyphenols in beans may limit the benefit of increased Fe-concentration. Therefore, specific targeting of such compounds during the breeding process may yield improved dietary Fe-bioavailability. Our findings are in agreement with the human efficacy trial that demonstrated that the biofortified carioca beans improved the Fe-status of Rwandan women. We suggest the utilization of these in vitro and in vivo screening tools to guide studies aimed to develop and evaluate biofortified staple food crops. This approach has the potential to more effectively utilize research funds and provides a means to monitor the nutritional quality of the Fe-biofortified crops once released to farmers. C1 [Tako, Elad; Reed, Spenser; Hart, Jonathan J.; Glahn, Raymond P.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Anandaraman, Amrutha] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Beebe, Steve E.] CIAT Int Ctr Trop Agr, Cali 6713, Colombia. RP Tako, E (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM et79@cornell.edu NR 56 TC 6 Z9 6 U1 1 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 18 PY 2015 VL 10 IS 9 AR e0138479 DI 10.1371/journal.pone.0138479 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS0ZE UT WOS:000361790200112 PM 26381264 ER PT J AU Wang, HF Jacobs, DR Gaffo, AL Gross, MD Goff, DC Carr, JJ AF Wang, Huifen Jacobs, David R., Jr. Gaffo, Angelo L. Gross, Myron D. Goff, David C., Jr. Carr, J. Jeffrey TI Serum Urate and Incident Cardiovascular Disease: The Coronary Artery Risk Development in Young Adults (CARDIA) Study SO PLOS ONE LA English DT Article ID URIC-ACID LEVELS; HEART-DISEASE; SUBCLINICAL ATHEROSCLEROSIS; BLOOD-PRESSURE; HYPERURICEMIA; METAANALYSIS; ASSOCIATION; MORTALITY; PLASMA; INSULIN AB Objective There is controversy about whether serum urate (sUA) predicts future cardiovascular disease (CVD) independently of classical risk factors, and the age at which any prediction starts. We studied the sUA-CVD association among generally healthy adults. Methods CARDIA recruited 5115 black and white individuals aged 18-30 years in 1985-1986 (year-). Fatal and nonfatal CVD events by year 27 (n = 164) were ascertained during annual contacts and classified using medical records. The association with sUA (year-0, 10, 15 and 20) was modeled using Cox proportional hazards regression, pooling over gender-specific quartiles. Results Mean sUA concentration was higher in men than women, but increased over time in both genders. Those with elevated sUA had worse metabolic profiles that substantially deteriorated over time. Adjusting for demographic and lifestyle factors (the minimal model), baseline sUA concentration was positively associated with incident CVD (hazard ratio (HR) per mg/dL = 1.21; 95% confidence interval: 1.05, 1.39; P = 0.005). This positive association attenuated to nonsignificance in the full model accounting simultaneously for classical CVD risk factors (HR = 1.09; 0.94, 1.27; P = 0.24). Both the minimal and full models appeared to show stronger associations (than year-0 sUA) between year-10 sUA and incident CVD (HR = 1.27 and 1.12, respectively), but sUA was not statistically significant in the full model. Despite fewer events, year-15 sUA showed a significant sUA-CVD association pattern, with minimal model association magnitude comparable to year-10, and remained significant in the full model (HR = 1.19; 1.02, 1.40; P = 0.03). Hyperuricemia at year-15 strongly predicted CVD risk (HR = 2.11; 1.34, 3.33; P = 0.001), with some attenuation in the full model (HR = 1.68; P = 0.04). Conclusions sUA may be an early biomarker for CVD in adults entering middle age. The prediction of CVD by sUA appeared to strengthen with aging. The potential complex relation of sUA with deterioration of a cluster of metabolic abnormalities warrants future exploration. C1 [Wang, Huifen] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Lab, Boston, MA 02111 USA. [Jacobs, David R., Jr.] Univ Minnesota Twin Cities, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55455 USA. [Gaffo, Angelo L.] Univ Alabama Birmingham, Div Rheumatol, Birmingham, AL USA. [Gross, Myron D.] Univ Minnesota Twin Cities, Lab Med & Pathol, Minneapolis, MN USA. [Goff, David C., Jr.] Univ Colorado Denver, Colorado Sch Publ Hlth, Aurora, CO USA. [Carr, J. Jeffrey] Vanderbilt Univ, Med Ctr, Dept Radiol, Nashville, TN 37232 USA. RP Jacobs, DR (reprint author), Univ Minnesota Twin Cities, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55455 USA. EM jacob004@umn.edu FU National Heart, Lung, and Blood Institute [HHSN268201300025C, HHSN268201300026C, HHSN268201300027C, HHSN268201300028C, HHSN268201300029C, HHSN268200900041C, RO1- HL53560]; Cymabay, Inc FX CARDIA is supported by contracts HHSN268201300025C, HHSN268201300026C, HHSN268201300027C, HHSN268201300028C, HHSN268201300029C, and HHSN268200900041C from the National Heart, Lung, and Blood Institute. DRJ and MDG are the joint principal investigators for YALTA supported by RO1- HL53560 from the National Heart, Lung, and Blood Institute. The funding organization participated in the design of study, choice of enrolled patients, and review and interpretation of data. The CARDIA Publications and Presentations Committee, in which National Heart, Lung, and Blood Institute and academic members participate as equal partners, reviewed and approved the manuscript. Cymabay, Inc provided support in the form of consulting fees for activities unrelated to this paper or its general topic for one author (ALG), but did not have any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of this author is articulated in the "author contributions" section. NR 43 TC 2 Z9 2 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 18 PY 2015 VL 10 IS 9 AR e0138067 DI 10.1371/journal.pone.0138067 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CS0ZE UT WOS:000361790200054 PM 26381512 ER PT J AU Fricke, WF Song, Y Wang, AJ Smith, A Grinchuk, V Pei, CL Ma, B Lu, NH Urban, JF Shea-Donohue, T Zhao, AP AF Fricke, W. Florian Song, Yang Wang, An-Jiang Smith, Allen Grinchuk, Viktoriya Pei, Chenlin Ma, Bing Lu, Nonghua Urban, Joseph F., Jr. Shea-Donohue, Terez Zhao, Aiping TI Type 2 immunity-dependent reduction of segmented filamentous bacteria in mice infected with the helminthic parasite Nippostrongylus brasiliensis SO MICROBIOME LA English DT Article DE Helminth parasite; Nippostrongylus brasiliensis; Type 2 immunity; IL-13; STAT6; Antimicrobial peptides; Segmented filamentous bacteria; IL-17; Microbiota ID MURINE GASTROINTESTINAL-TRACT; TRICHURIS-SUIS THERAPY; GUT MICROBIOTA; HELIGMOSOMOIDES-POLYGYRUS; HOST; CELLS; DISEASE; TH17; COLONIZATION; INFLAMMATION AB Background: Dynamic interactions between the host and gastrointestinal microbiota play an important role for local and systemic immune homeostasis. Helminthic parasites modulate the host immune response, resulting in protection against autoimmune disease but also increased susceptibility to pathogen infection. The underlying mechanisms remain largely unknown. Results: We showed that the type 2 immune response to enteric Nippostrongylus brasiliensis infection in mice was associated with altered intestinal mucin and AMP expression and shifts in microbiota composition. Most strikingly, infection reduced concentrations of intestinal segmented filamentous bacteria (SFB), known inducers of T helper 17 cells, and IL-17-associated gene expression. Infected mice deficient in IL-13 or STAT6 did not reduce SFB or IL-17, and exogenous IL-25 replicated the effects of parasite infection in wild type mice. Conclusions: Our data show that parasite infection acts through host type 2 immunity to reduce intestinal SFB and expression of IL-17, providing an example of a microbiota-dependent immune modulation by parasites. C1 [Fricke, W. Florian; Song, Yang; Ma, Bing] Univ Maryland, Sch Med, Dept Microbiol & Immunol, Inst Genome Sci, Baltimore, MD 21201 USA. [Fricke, W. Florian] Univ Hohenheim, Dept Nutrigen, Stuttgart, Germany. [Wang, An-Jiang; Grinchuk, Viktoriya; Pei, Chenlin; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Dept Radiat Oncol, Baltimore, MD 21201 USA. [Smith, Allen; Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. [Wang, An-Jiang; Lu, Nonghua] Nanchang Univ, Affiliated Hosp 1, Dept Gastroenterol & Hepatol, Nanchang, Peoples R China. RP Fricke, WF (reprint author), Univ Maryland, Sch Med, Dept Microbiol & Immunol, Inst Genome Sci, Baltimore, MD 21201 USA. EM w.florian.fricke@uni-hohenheim.de; azhao@mbrc.umaryland.edu RI song, yang/P-1033-2015; OI Fricke, W. Florian/0000-0003-1586-0962 FU Carl-Zeiss-Stiftung/German Scholars Organization e.V. (Wissenschaftler-Ruckkehrprogramm GSO/CZS); U.S. National Institutes of Health [R01-DK083418, R01-AI/DK49316]; U.S. Department of Agriculture (CRIS) [1235-51000-055]; Chinese State Scholarship Fund [201208360042] FX This work was supported by the Carl-Zeiss-Stiftung/German Scholars Organization e.V. (WFF: Wissenschaftler-Ruckkehrprogramm GSO/CZS), the U.S. National Institutes of Health (AZ: R01-DK083418, T.S-D: R01-AI/DK49316), the U.S. Department of Agriculture (AS, JFU: CRIS #1235-51000-055), and the Chinese State Scholarship Fund (AJW: #201208360042). NR 59 TC 3 Z9 3 U1 0 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2049-2618 J9 MICROBIOME JI Microbiome PD SEP 17 PY 2015 VL 3 AR 40 DI 10.1186/s40168-015-0103-8 PG 12 WC Microbiology SC Microbiology GA CU0WJ UT WOS:000363239300001 PM 26377648 ER PT J AU Luo, MC You, FM Li, PC Wang, JR Zhu, TT Dandekar, AM Leslie, CA Aradhya, M McGuire, PE Dvorak, J AF Luo, Ming-Cheng You, Frank M. Li, Pingchuan Wang, Ji-Rui Zhu, Tingting Dandekar, Abhaya M. Leslie, Charles A. Aradhya, Mallikarjuna McGuire, Patrick E. Dvorak, Jan TI Synteny analysis in Rosids with a walnut physical map reveals slow genome evolution in long-lived woody perennials SO BMC GENOMICS LA English DT Article DE Fagales; Angiosperm; Dysploidy; Polyploidy; Juglandaceae; Recombination rate; Single nucleotide polymorphism; Molecular clock ID GENETIC-LINKAGE MAPS; X DOMESTICA BORKH.; BRACHYPODIUM-DISTACHYON; TRANSPOSABLE ELEMENTS; MEIOTIC RECOMBINATION; PHYLOGENETIC ANALYSES; AEGILOPS-TAUSCHII; MOLECULAR CLOCK; GENERATION TIME; DNA-SEQUENCES AB Background: Mutations often accompany DNA replication. Since there may be fewer cell cycles per year in the germlines of long-lived than short-lived angiosperms, the genomes of long-lived angiosperms may be diverging more slowly than those of short-lived angiosperms. Here we test this hypothesis. Results: We first constructed a genetic map for walnut, a woody perennial. All linkage groups were short, and recombination rates were greatly reduced in the centromeric regions. We then used the genetic map to construct a walnut bacterial artificial chromosome (BAC) clone-based physical map, which contained 15,203 exonic BAC-end sequences, and quantified with it synteny between the walnut genome and genomes of three long-lived woody perennials, Vitis vinifera, Populus trichocarpa, and Malus domestica, and three short-lived herbs, Cucumis sativus, Medicago truncatula, and Fragaria vesca. Each measure of synteny we used showed that the genomes of woody perennials were less diverged from the walnut genome than those of herbs. We also estimated the nucleotide substitution rate at silent codon positions in the walnut lineage. It was one-fifth and one-sixth of published nucleotide substitution rates in the Medicago and Arabidopsis lineages, respectively. We uncovered a whole-genome duplication in the walnut lineage, dated it to the neighborhood of the Cretaceous-Tertiary boundary, and allocated the 16 walnut chromosomes into eight homoeologous pairs. We pointed out that during polyploidy-dysploidy cycles, the dominant tendency is to reduce the chromosome number. Conclusion: Slow rates of nucleotide substitution are accompanied by slow rates of synteny erosion during genome divergence in woody perennials. C1 [Luo, Ming-Cheng; Wang, Ji-Rui; Zhu, Tingting; Dandekar, Abhaya M.; Leslie, Charles A.; McGuire, Patrick E.; Dvorak, Jan] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [You, Frank M.; Li, Pingchuan] Agr & Agri Food Canada, Cereal Res Ctr, Morden, MB, Canada. [Aradhya, Mallikarjuna] USDA ARS, Clonal Repository, Davis, CA USA. [Wang, Ji-Rui] Sichuan Agr Univ, Triticeae Res Inst, Wenjiang, Peoples R China. RP Dvorak, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM jdvorak@ucdavis.edu OI Dandekar, Abhaya/0000-0001-7925-4086 FU UC Discovery Program; California Walnut Board FX We thank Yuqin Hu for dedicated technical assistance with BAC clone fingerprinting. We also thank the UC Discovery Program and California Walnut Board for funding this study and David Ramos, Research Director for the California Walnut Board, for facilitating funding of this project from California Walnut Board. NR 87 TC 2 Z9 2 U1 5 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD SEP 17 PY 2015 VL 16 AR 707 DI 10.1186/s12864-015-1906-5 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CR5BG UT WOS:000361353400003 PM 26383694 ER PT J AU Feenstra, F Drolet, BS Boonstra, J van Rijn, PA AF Feenstra, Femke Drolet, Barbara S. Boonstra, Jan van Rijn, Piet A. TI Non-structural protein NS3/NS3a is required for propagation of bluetongue virus in Culicoides sonorensis SO PARASITES & VECTORS LA English DT Article DE Bluetongue virus; Culicoides; Arbovirus; DISA vaccine; NS3/NS3a; Midge ID AFRICAN HORSE SICKNESS; VARIIPENNIS DIPTERA; SCHMALLENBERG VIRUS; CELL-LINES; RNA INTERFERENCE; INFECTED-CELLS; BITING MIDGES; CERATOPOGONIDAE; VECTOR; RELEASE AB Background: Bluetongue virus (BTV) causes non-contagious haemorrhagic disease in ruminants and is transmitted by Culicoides spp. biting midges. BTV encodes four non-structural proteins of which NS3/NS3a is functional in virus release. NS3/NS3a is not essential for in vitro virus replication. However, deletion of NS3/NS3a leads to delayed virus release from mammalian cells and largely reduces virus release from insect cells. NS3/NS3a knockout BTV in sheep causes no viremia, but induces sterile immunity and is therefore proposed to be a Disabled Infectious Single Animal (DISA) vaccine candidate. In the absence of viremia, uptake of this vaccine strain by blood-feeding midges would be highly unlikely. Nevertheless, unintended replication of vaccine strains within vectors, and subsequent recombination or re-assortment resulting in virulent phenotypes and transmission is a safety concern of modified-live vaccines. Methods: The role of NS3/NS3a in replication and dissemination of BTV1, expressing VP2 of serotype 2 within colonized Culicoides sonorensis midges was investigated. Virus strains were generated using reverse genetics and their growth was examined in vitro. A laboratory colony of C. sonorensis, a known competent BTV vector, was fed or injected with BTV with or without expressing NS3/NS3a and replication in the midge was examined using RT PCR. Crossing of the midgut infection barrier was examined by separate testing of midge heads and bodies. Results: Although the parental NS3/NS3a expressing strain was not able to replicate and disseminate within C. sonorensis after oral feeding, this virus was able to replicate efficiently when the midgut infection barrier was bypassed by intrathoracic injection, whereas the NS3/NS3a knockout mutant was unable to replicate. This demonstrates that NS3/NS3a is required for viral replication within Culicoides. Conclusion: The lack of viremia and the inability to replicate within the vector, clearly demonstrate the inability of NS3/NS3a knockout DISA vaccine strains to be transmitted by midges. C1 [Feenstra, Femke; Boonstra, Jan; van Rijn, Piet A.] Cent Vet Inst Wageningen UR, Dept Virol, Lelystad, Netherlands. [Feenstra, Femke] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Utrecht, Netherlands. [Drolet, Barbara S.] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS USA. [van Rijn, Piet A.] North West Univ, Ctr Human Metabon, Dept Biochem, Potchefstroom, South Africa. RP van Rijn, PA (reprint author), Cent Vet Inst Wageningen UR, Dept Virol, Lelystad, Netherlands. EM Piet.vanRijn@wur.nl OI van Rijn, Piet A./0000-0002-2594-1232 FU Dutch Ministry of Economic Affairs (CVI-project) [1600020-01]; USDA, ARS, NP103 Animal Health National Program [3020-32000-006] FX This study was funded by the Dutch Ministry of Economic Affairs (CVI-project 1600020-01) and USDA, ARS, NP103 Animal Health National Program (Project Number 3020-32000-006). We would like to thank Mr. Rene van Gennip for fruitful discussions, Dr. Paul Wichers-Schreur for help with graphics and Prof. Rob Moormann for critical reading of the manuscript. We also thank Dr. Lee Cohnstaedt, Mr. James Kempert and Mr. William Yarnell at the Arthropod-Borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS for providing all C. sonorensis midges. We are grateful to Dr. Paloma Rueda (Ingenasa, Madrid, Spain) for the generous gift of MAbs. NR 62 TC 5 Z9 5 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD SEP 17 PY 2015 VL 8 AR 476 DI 10.1186/s13071-015-1063-3 PG 9 WC Parasitology SC Parasitology GA CR6KX UT WOS:000361456800021 PM 26383094 ER PT J AU Palti, Y Vallejo, RL Gao, GT Liu, SX Hernandez, AG Rexroad, CE Wiens, GD AF Palti, Yniv Vallejo, Roger L. Gao, Guangtu Liu, Sixin Hernandez, Alvaro G. Rexroad, Caird E., III Wiens, Gregory D. TI Detection and Validation of QTL Affecting Bacterial Cold Water Disease Resistance in Rainbow Trout Using Restriction-Site Associated DNA Sequencing SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCI; SALMON SALMO-SALAR; ONCORHYNCHUS-MYKISS; FLAVOBACTERIUM-PSYCHROPHILUM; STEELHEAD TROUT; LINKAGE MAPS; FISH; IDENTIFICATION; POPULATIONS; STRESS AB Bacterial cold water disease (BCWD) causes significant economic loss in salmonid aquaculture. Using microsatellite markers in a genome scan, we previously detected significant and suggestive QTL affecting phenotypic variation in survival following challenge with Flavobacterium psychrophilum, the causative agent of BCWD in rainbow trout. In this study, we performed selective genotyping of SNPs from restriction-site associated DNA (RAD) sequence data from two pedigreed families (2009070 and 2009196) to validate the major QTL from the previous work and to detect new QTL. The use of RAD SNPs in the genome scans increased the number of mapped markers from similar to 300 to similar to 5,000 per family. The significant QTL detected in the microsatellites scan on chromosome Omy8 in family 2009070 was validated explaining up to 58% of the phenotypic variance in that family, and in addition, a second QTL was also detected on Omy8. Two novel QTL on Omy11 and 14 were also detected, and the previously suggestive QTL on Omy1, 7 and 25 were also validated in family 2009070. In family 2009196, the microsatellite significant QTL on Omy6 and 12 were validated and a new QTL on Omy8 was detected, but none of the previously detected suggestive QTL were validated. The two Omy8 QTL from family 2009070 and the Omy12 QTL from family 2009196 were found to be co-localized with handling and confinement stress response QTL that our group has previously identified in a separate pedigreed family. With the currently available data we cannot determine if the co-localized QTL are the result of genes with pleiotropic effects or a mere physical proximity on the same chromosome segment. The genetic markers linked to BCWD resistance QTL were used to query the scaffolds of the rainbow trout reference genome assembly and the QTL-positive scaffold sequences were found to include 100 positional candidate genes. Several of the candidate genes located on or near the two Omy8 QTL detected in family 2009070 suggest potential linkages between stress response and the regulation of immune response in rainbow trout. C1 [Palti, Yniv; Vallejo, Roger L.; Gao, Guangtu; Liu, Sixin; Rexroad, Caird E., III; Wiens, Gregory D.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Hernandez, Alvaro G.] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. RP Palti, Y (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM yniv.palti@ars.usda.gov FU USDA Agricultural Research Service, CRIS Project [1930-31000-012] FX This work was funded by USDA Agricultural Research Service, CRIS Project 1930-31000-012 (http://www.ars.usda.gov/research/projects/projects.htm?accn_no=427931). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 11 Z9 11 U1 4 U2 32 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 16 PY 2015 VL 10 IS 9 AR e0138435 DI 10.1371/journal.pone.0138435 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8NH UT WOS:000361610200108 PM 26376182 ER PT J AU Gliniewicz, K Wildung, M Orfe, LH Wiens, GD Cain, KD Lahmers, KK Snekvik, KR Call, DR AF Gliniewicz, Karol Wildung, Mark Orfe, Lisa H. Wiens, Gregory D. Cain, Kenneth D. Lahmers, Kevin K. Snekvik, Kevin R. Call, Douglas R. TI Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum SO BMC MICROBIOLOGY LA English DT Article DE Rifampicin; Attenuation; Flavobacterium psychrophilum; SNP; Methylome ID EDWARDSIELLA-ICTALURI VACCINE; BACTERIAL RNA-POLYMERASE; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; MYCOBACTERIUM-TUBERCULOSIS; CAROTENOID BIOSYNTHESIS; TRANSPOSON MUTAGENESIS; AEROMONAS-HYDROPHILA; ENTERIC SEPTICEMIA; CHANNEL CATFISH AB Background: Flavobacterium psychrophilum is the etiologic agent of bacterial coldwater disease in salmonids. Earlier research showed that a rifampicin-passaged strain of F. psychrophilum (CSF 259-93B. 17) caused no disease in rainbow trout (Oncorhynchus mykiss, Walbaum) while inducing a protective immune response against challenge with the virulent CSF 259-93 strain. We hypothesized that rifampicin passage leads to an accumulation of genomic mutations that, by chance, reduce virulence. To assess the pattern of phenotypic and genotypic changes associated with passage, we examined proteomic, LPS and single-nucleotide polymorphism (SNP) differences for two F. psychrophilum strains (CSF 259-93 and THC 02-90) that were passaged with and without rifampicin selection. Results: Rifampicin resistance was conveyed by expected mutations in rpoB, although affecting different DNA bases depending on the strain. One rifampicin-passaged CSF 259-93 strain (CR) was attenuated (4 % mortality) in challenged fish, but only accumulated eight nonsynonymous SNPs compared to the parent strain. A CSF 259-93 strain passaged without rifampicin (CN) accumulated five nonsynonymous SNPs and was partially attenuated (28 % mortality) compared to the parent strain (54.5 % mortality). In contrast, there were no significant change in fish mortalities among THC 02-90 wild-type and passaged strains, despite numerous SNPs accumulated during passage with (n = 174) and without rifampicin (n = 126). While only three missense SNPs were associated with attenuation, a Ser492Phe rpoB mutation in the CR strain may contribute to further attenuation. All strains except CR retained a gliding motility phenotype. Few proteomic differences were observed by 2D SDS-PAGE and there were no apparent changes in LPS between strains. Comparative methylome analysis of two strains (CR and TR) identified no shared methylation motifs for these two strains. Conclusion: Multiple genomic changes arose during passage experiments with rifampicin selection pressure. Consistent with our hypothesis, unique strain-specific mutations were detected for the fully attenuated (CR), partially attenuated (CN) and another fully attenuated strain (B17). C1 [Gliniewicz, Karol; Snekvik, Kevin R.; Call, Douglas R.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Wildung, Mark] Washington State Univ, Mol Biol & Genom Core, Pullman, WA 99164 USA. [Wiens, Gregory D.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Leetown, WV USA. [Cain, Kenneth D.] Univ Idaho, Dept Fish & Wildlife Resources, Moscow, ID USA. [Lahmers, Kevin K.] Virginia Polytech Inst & State Univ, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. [Snekvik, Kevin R.] Washington State Univ, Washington Anim Dis Diagnost Lab, Pullman, WA 99164 USA. [Lahmers, Kevin K.; Call, Douglas R.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. RP Call, DR (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM drcall@wsu.edu FU University of Idaho/Washington State University Aquaculture Initiative; Western Regional Aquaculture Center; Washington State Agricultural Research Center FX Dr. S. Aguilar from Washington State University, and Dr. A. Long and T. Fehringer from University Idaho provided technical assistance. Dr. C. Moffitt and Dr. Z. Penney from University of Idaho provided help with micrographs. This project was supported in part by the University of Idaho/Washington State University Aquaculture Initiative, the Western Regional Aquaculture Center, and Washington State Agricultural Research Center. NR 60 TC 0 Z9 0 U1 1 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD SEP 16 PY 2015 VL 15 AR 179 DI 10.1186/s12866-015-0518-1 PG 15 WC Microbiology SC Microbiology GA CR6IC UT WOS:000361448300001 PM 26377311 ER PT J AU Mahmud, I Kousik, C Hassell, R Chowdhury, K Boroujerdi, AF AF Mahmud, Iqbal Kousik, Chandrasekar Hassell, Richard Chowdhury, Kamal Boroujerdi, Arezue F. TI NMR Spectroscopy Identifies Metabolites Trans located from Powdery Mildew Resistant Rootstocks to Susceptible Watermelon Scions SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE grafted watermelon; powdery mildew disease; nuclear magnetic resonance; multivariate principal component analysis; biochemical pathway analysis ID PODOSPHAERA-XANTHII; METABOLOMICS; MELATONIN; TISSUE; DISEASE; AUXIN; DISCRIMINATION; EXTRACTION; CULTIVARS; PROFILE AB Powdery mildew (PM) disease causes significant loss in watermelon. Due to the unavailability of a commercial watermelon variety that is resistant to PM, grafting susceptible cultivars on wild resistant rootstocks is being explored as a short-term management strategy to combat this disease. Nuclear magnetic resonance-based metabolic profiles of susceptible and resistant rootstocks of watermelon and their corresponding susceptible scions (Mickey Lee) were compared to screen for potential metabolites related to PM resistance using multivariate principal component analysis. Significant score plot differences between the susceptible and resistant groups were revealed through Mahalanobis distance analysis. Significantly different spectral buckets and their corresponding metabolites (including choline, fumarate, 5-hydroxyindole-3-acetate, and melatonin) have been identified quantitatively using multivariate loading plots and verified by volcano plot analyses. The data suggest that these metabolites were translocated from the powdery mildew resistant rootstocks to their corresponding powdery mildew susceptible scions and can be related to PM disease resistance. C1 [Mahmud, Iqbal; Chowdhury, Kamal] Claflin Univ, Sch Nat Sci & Math, Dept Biol, Orangeburg, SC 29115 USA. [Mahmud, Iqbal; Boroujerdi, Arezue F.] Claflin Univ, Dept Chem, Mol Sci Res Ctr, Orangeburg, SC 29115 USA. [Kousik, Chandrasekar] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Hassell, Richard] Clemson Univ, Dept Agr Forest & Environm Sci, Coastal Res & Educ Ctr, Clemson, SC 29634 USA. RP Boroujerdi, AF (reprint author), Claflin Univ, Dept Chem, Mol Sci Res Ctr, Orangeburg, SC 29115 USA. EM aboroujerdi@claflin.edu FU SC-INBRE [2 P20 GM103499] FX A.F.B. is supported by SC-INBRE (2 P20 GM103499). Mild Watanabe is thanked for her methods development work. Jennifer Ikerd and Mark Schaffer of USDA are thanked for their assistance. No trademark names or proprietary products constitute a guarantee of the product by USDA, ARS, Clemson University or Claflin University, nor do they imply exclusion of other suitable products. NR 46 TC 1 Z9 1 U1 1 U2 28 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD SEP 16 PY 2015 VL 63 IS 36 BP 8083 EP 8091 DI 10.1021/acs.jafc.5b02108 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CR7BD UT WOS:000361502000024 PM 26302171 ER PT J AU Staehelin, C Krishnan, HB AF Staehelin, Christian Krishnan, Hari B. TI Nodulation outer proteins: double-edged swords of symbiotic rhizobia SO BIOCHEMICAL JOURNAL LA English DT Review DE effector; nodulation outer protein; plant defence; root nodule; symbiosis; type III secretion system ID III SECRETION SYSTEM; SINORHIZOBIUM-FREDII USDA257; SP STRAIN NGR234; CULTIVAR-SPECIFIC NODULATION; GRAM-NEGATIVE BACTERIA; BRADYRHIZOBIUM-JAPONICUM; FUNCTIONAL-ANALYSIS; SURFACE APPENDAGES; MESORHIZOBIUM-LOTI; EFFECTOR PROTEINS AB Rhizobia are nitrogen-fixing bacteria that establish a nodule symbiosis with legumes. Nodule formation depends on signals and surface determinants produced by both symbiotic partners. Among them, rhizobial Nops (nodulation outer proteins) play a crucial symbiotic role in many strain-host combinations. Nops are defined as proteins secreted via a rhizobial T3SS (type III secretion system). Functional T3SSs have been characterized in many rhizobial strains. Nops have been identified using various genetic, biochemical, proteomic, genomic and experimental approaches. Certain Nops represent extracellular components of the T3SS, which are visible in electron micrographs as bacterial surface appendages called T3 (type III) pili. Other Nops are T3 effector proteins that can be translocated into plant cells. Rhizobial T3 effectors manipulate cellular processes in host cells to suppress plant defence responses against rhizobia and to promote symbiosis-related processes. Accordingly, mutant strains deficient in synthesis or secretion of T3 effectors show other hand, direct or indirect recognition of T3 effectors by plant cells expressing specific R (resistance) proteins can result in effector triggered defence responses that negatively affect rhizobial infection. Hence Nops are double-edged swords that may promote establishment of symbiosis with one legume (symbiotic factors) and impair symbiotic processes when bacteria are inoculated on another legume species (asymbiotic factors). In the present review, we provide an overview of our current understanding of Nops. We summarize their symbiotic effects, their biochemical properties and their possible modes of action. Finally, we discuss future perspectives in the field of T3 effector research. C1 [Staehelin, Christian] Sun Yat Sen Univ, Sch Life Sci, State Key Lab, East Campus, Guangzhou 510006, Guangdong, Peoples R China. [Staehelin, Christian] Sun Yat Sen Univ, Sch Life Sci, Guangdong Key Lab Plant Resources, Guangzhou 510006, Guangdong, Peoples R China. [Staehelin, Christian] Sun Yat Sen Univ, Sch Life Sci, Shenzhen Res & Dev Ctr, State Key Lab Biocontrol, Shenzhen 518057, Peoples R China. [Krishnan, Hari B.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Staehelin, C (reprint author), Sun Yat Sen Univ, Sch Life Sci, State Key Lab, East Campus, Guangzhou 510006, Guangdong, Peoples R China. EM cst@mail.sysu.edu.cn FU National Natural Science Foundation of China [31270128, 31070219]; Department of Science and Technology of Guangdong Province, China [2009B030802007]; Science Foundation of the State Key Laboratory of Biocontrol [SKLBC201123]; Guangdong Key Laboratory of Plant Resources [plant01k19]; U.S. Department of Agriculture - Agricultural Research Service FX Research of C.S. is funded by the National Natural Science Foundation of China [grant numbers 31270128 and 31070219], by the Department of Science and Technology of Guangdong Province, China [grant number 2009B030802007], by the Science Foundation of the State Key Laboratory of Biocontrol [grant number SKLBC201123] and by the Guangdong Key Laboratory of Plant Resources [grant number plant01k19]. The U.S. Department of Agriculture - Agricultural Research Service provided funding for research by H.B.K. NR 108 TC 4 Z9 4 U1 6 U2 17 PU PORTLAND PRESS LTD PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND SN 0264-6021 EI 1470-8728 J9 BIOCHEM J JI Biochem. J. PD SEP 15 PY 2015 VL 470 BP 263 EP 274 DI 10.1042/BJ20150518 PN 3 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DC4BQ UT WOS:000369165300003 PM 26341483 ER PT J AU Yang, JY Fleisher, DH Sicher, RC Kim, J Baligar, VC Reddy, VR AF Yang, Jinyoung Fleisher, David H. Sicher, Richard C. Kim, Joonyup Baligar, Virupax C. Reddy, Vangimalla R. TI Effects of CO2 enrichment and drought pretreatment on metabolite responses to water stress and subsequent rehydration using potato tubers from plants grown in sunlit chambers SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Abiotic stress; Osmolytes; Soluble carbohydrates; Plant metabolism; Water potential ID FREE AMINO-ACIDS; CARBON-DIOXIDE; SOLANUM-TUBEROSUM; ARABIDOPSIS-THALIANA; ACCLIMATION; YIELD; OSMOREGULATION; PHOTOSYNTHESIS; TEMPERATURE; TOLERANCE AB Experiments were performed using naturally sunlit Soil-Plant-Atmosphere Research chambers that provided ambient or twice ambient CO2. Potato plants were grown in pots that were water sufficient (W), water insufficient for 12-18 days during both vegetative and tuber development stages (VR), or water insufficient solely during tuber development (R). In the ambient CO2 treatment, a total of 17 and 20 out of 31 tuber metabolites differed when comparing the W to the R and VR treatments, respectively. Hexoses, raffinose, mannitol, branched chain amino acids, phenylalanine and proline increased, although most organic acids remained unchanged or decreased in response to drought. Osmolytes, including glucose, branched chain amino acids and proline, remained elevated following 2 weeks of rehydration in both the ambient and elevated CO2 treatments, whereas fructose, raffinose, mannitol and some organic acids reverted to control levels. Failure of desiccated plant tissues to mobilize specific osmolytes after rehydration was unexpected and was likely because tubers function as terminal sinks. Tuber metabolite responses to single or double drought treatments were similar under the same CO2 levels but important differences were noted when CO2 level was varied. We also found that metabolite changes to water insufficiency and/or CO2 enrichment were very distinct between sink and source tissues, and total metabolite changes to stress were generally greater in leaflets than tubers. Published by Elsevier GmbH. C1 [Yang, Jinyoung; Fleisher, David H.; Sicher, Richard C.; Reddy, Vangimalla R.] ARS, USDA, Crop Syst & Global Change Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Kim, Joonyup] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Baligar, Virupax C.] ARS, USDA, Sustainable Perennial Crops Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. RP Yang, JY (reprint author), ARS, USDA, Crop Syst & Global Change Lab, Beltsville Agr Res Ctr West, Room 342,Bldg 001,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM jinyoung.barnaby@ars.usda.gov FU USDA (CRIS) [8042-11210-001-00D (01)] FX The authors thank Robert Erdman for assistance with metabolite measurements and Emily Morris-Warnock and Jackson Fisher for assistance in operating the SPAR chambers. This work was funded by USDA under grant number CRIS # 8042-11210-001-00D (01). NR 51 TC 1 Z9 1 U1 8 U2 20 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD SEP 15 PY 2015 VL 189 BP 126 EP 136 DI 10.1016/j.jplph.2015.10.004 PG 11 WC Plant Sciences SC Plant Sciences GA CZ3FA UT WOS:000366988600014 PM 26600557 ER PT J AU Kinzner, MC Wagner, HC Peskoller, A Moder, K Dowell, FE Arthofer, W Schlick-Steiner, BC Steiner, FM AF Kinzner, Martin-Carl Wagner, Herbert C. Peskoller, Andrea Moder, Karl Dowell, Floyd E. Arthofer, Wolfgang Schlick-Steiner, Birgit C. Steiner, Florian M. TI A near-infrared spectroscopy routine for unambiguous identification of cryptic ant species SO PEERJ LA English DT Article DE Cryptic-species complex; Ants; Formicidae; Neural networks; One-vs-all strategy; Partial least squares regression; Random forests; Species identification tool; Tetramorium ID RANDOM FOREST; HYMENOPTERA-FORMICIDAE; REFLECTANCE SPECTROSCOPY; CLASSIFICATION; DIVERSITY; AGE; ORIGIN; TOOL; DIFFERENTIATION; TAXONOMY AB Species identification-of importance for most biological disciplines-is not always straightforward as cryptic species hamper traditional identification. Fibre-optic near-infrared spectroscopy (NIRS) is a rapid and inexpensive method of use in various applications, including the identification of species. Despite its efficiency, NIRS has never been tested on a group of more than two cryptic species, and a working routine is still missing. Hence, we tested if the four morphologically highly similar, but genetically distinct ant species Tetramorium alpestre, T. caespitum, T. impurum, and T. sp. B, all four co-occurring above 1,300 m above sea level in the Alps, can be identified unambiguously using NIRS. Furthermore, we evaluated which of our implementations of the three analysis approaches, partial least squares regression (PLS), artificial neural networks (ANN), and randomforests (RF), is most efficient in species identification with our data set. We opted for a 100% classification certainty, i.e., a residual risk of misidentification of zero within the available data, at the cost of excluding specimens from identification. Additionally, we examined which strategy among our implementations, one-vs-all, i.e., one species compared with the pooled set of the remaining species, or binary-decision strategies, worked best with our data to reduce amulti-class systemto a two-class system, as is necessary for PLS. Our NIRS identification routine, based on a 100% identification certainty, was successful with up to 66.7% of unambiguously identified specimens of a species. In detail, PLS scored best over all species (36.7% of specimens), while RF was much less effective (10.0%) and ANN failed completely (0.0%) with our data and our implementations of the analyses. Moreover, we showed that the one-vs-all strategy is the only acceptable option to reduce multi-class systems because of a minimum expenditure of time. We emphasise our classification routine using fibre-optic NIRS in combination with PLS and the one-vs-all strategy as a highly efficient pre-screening identification method for cryptic ant species and possibly beyond. C1 [Kinzner, Martin-Carl; Wagner, Herbert C.; Peskoller, Andrea; Arthofer, Wolfgang; Schlick-Steiner, Birgit C.; Steiner, Florian M.] Univ Innsbruck, Inst Ecol, Mol Ecol Grp, A-6020 Innsbruck, Austria. [Moder, Karl] Univ Nat Resources & Life Sci, Inst Appl Stat & Comp, Vienna, Austria. [Dowell, Floyd E.] Agr Res Serv, USDA, Manhattan, KS USA. RP Kinzner, MC (reprint author), Univ Innsbruck, Inst Ecol, Mol Ecol Grp, A-6020 Innsbruck, Austria. EM martin-carl.kinzner@uibk.ac.at OI Kinzner, Martin-Carl/0000-0001-5195-4048 FU University of Innsbruck; Austrian Science Fund (FWF) [P23409] FX This research was supported by the University of Innsbruck and the Austrian Science Fund (FWF, http://www.fwf.ac.at/) under grant P23409 awarded to BCSS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 51 TC 1 Z9 1 U1 1 U2 7 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD SEP 15 PY 2015 VL 3 AR e991 DI 10.7717/peerj.991 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV5PX UT WOS:000364324400001 PM 26734510 ER PT J AU Pastiu, AI Gyorke, A Kalmar, Z Bolfa, P Rosenthal, BM Oltean, M Villena, I Spinu, M Cozma, V AF Pastiu, Anamaria Ioana Gyoerke, Adriana Kalmar, Zsuzsa Bolfa, Pompei Rosenthal, Benjamin Martin Oltean, Miruna Villena, Isabelle Spinu, Marina Cozma, Vasile TI Toxoplasma gondii in horse meat intended for human consumption in Romania SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Horses; Romania; Seroprevalence; Bioassay ID INFECTION; PCR AB The prevalence of Toxoplasmn gondii, an economically important zoonotic protozoan, was investigated in horses slaughtered for export and human consumption in the North of Romania. Pairs of samples, sera and heart tissues, were collected from 82 slaughtered horses. Examination of horse sera by ELISA at a dilution of 1:10, and by modified agglutination test (MAT) at a dilution of 1:6, revealed that 32 (39%) and 31(37.8%) horses, respectively, had antibodies against T. gondii. Using polymerase chain reaction (PCR) analysis, T. gondii DNA was not found in any heart sample collected from horses. By bioassay in mice, we obtained viable isolates of T. gondii from two of ten horses determined to be strongly positive by serological assay/ELISA. The prevalence estimated in horses highlighted the potential risk for human contamination by consumption of raw or undercooked meat. (C) 2015 Elsevier B.V. All rights reserved. C1 [Pastiu, Anamaria Ioana; Spinu, Marina] Univ Agr Sci & Vet Med, Fac Vet Med, Dept Infect Dis, Cluj Napoca 400372, Romania. [Pastiu, Anamaria Ioana; Gyoerke, Adriana; Kalmar, Zsuzsa; Oltean, Miruna; Cozma, Vasile] Univ Agr Sci & Vet Med, Fac Vet Med, Parasitol & Parasit Dis Dept, Cluj Napoca 400372, Romania. [Bolfa, Pompei] Ross Univ, Sch Vet Med, Dept Biomed Sci, Basseterre, St Kitts & Nevi. [Bolfa, Pompei] Univ Agr Sci & Vet Med, Fac Vet Med, Dept Pathol, Cluj Napoca 400372, Romania. [Rosenthal, Benjamin Martin] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Villena, Isabelle] Univ Reims, Natl Reference Ctr Toxoplasmosis, Parasitol Lab, EA 3800,SFR CAP SANTE, Reims, France. RP Gyorke, A (reprint author), Univ Agr Sci & Vet Med, Fac Vet Med, Parasitol & Parasit Dis Dept, 3-5 Manastur St, Cluj Napoca 400372, Romania. EM titilincua@yahoo.com RI Kalmar, Zsuzsa/A-1791-2017; OI Rosenthal, Benjamin/0000-0002-0224-3773 FU European Social Fund, Human Resources Development Operational Programme [POSDRU/159/1.5/S/136893]; Executive Unit for Higher Education, Research, Development and Innovation Funding (UEFISCDI, Romania) [620/2013] FX The work was done under the frame of European Social Fund, Human Resources Development Operational Programme 2007-2013, project no. POSDRU/159/1.5/S/136893. This paper was published under the frame of project no. 620/2013 from the Executive Unit for Higher Education, Research, Development and Innovation Funding (UEFISCDI, Romania). NR 13 TC 0 Z9 0 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD SEP 15 PY 2015 VL 212 IS 3-4 BP 393 EP 395 DI 10.1016/j.vetpar.2015.07.024 PG 3 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CU2LQ UT WOS:000363355400049 PM 26238654 ER PT J AU Verma, SK Calero-Bernal, R Lovallo, MJ Sweeny, AR Grigg, ME Dubey, JP AF Verma, S. K. Calero-Bernal, R. Lovallo, M. J. Sweeny, A. R. Grigg, M. E. Dubey, J. P. TI Detection of Sarcocystis spp. infection in bobcats (Lynx rufus) SO VETERINARY PARASITOLOGY LA English DT Article DE Bobcat (Lynx rufus); S. neurona; S. fells; S. dasypi; Sarcocysts; PCR-DNA sequencing ID ARMADILLOS DASYPUS-NOVEMCINCTUS; 9-BANDED ARMADILLO; NEURONA; PREVALENCE; ENCEPHALOMYELITIS; CAT AB The protozoan Sarcocystis neurona is an important cause of severe clinical disease of horses (called equine protozoal myeloencephalitis, EPM), marine mammals, companion animals, and several species of wildlife animals in the Americas. The Virginia opossum (Didelphis virginiana) is its definitive host in the USA and other animals act as intermediate or aberrant hosts. Samples of tongue and heart from 35 bobcats hunted for fur and food from Mississippi State, USA in February, 2014 were used for the present study. Muscles were examined for Sarcocystis infection by microscopic examination of either unfixed muscle squash preparations or pepsin digests, by histopathology of fixed samples, and by molecular methods. Sarcotystis-like bradyzoites were found in digests of 14 hearts and 10 tongues of 35 bobcats. In histological sections, sarcocysts were found in 26 of 35 bobcats; all appeared relatively thin-walled similar to S. felis sarcocysts under light microscope at 1000x magnification. S. neurona-like sarcocysts having thickened villar tips were seen in unstained muscle squash of tongue of two bobcats and PCR-DNA sequencing identified them definitively as S. neurona-like parasites. DNA extracted from bradyzoites obtained from tongue and heart muscle digests was analyzed by PCR-DNA sequencing at the ITS1 locus. Results indicated the presence of S. neurona-like parasite in 26 of 35 samples. ITS1 sequences identical to S. dasypi were identified in 3 bobcats, 2 of which were also co-infected with S. neurona-like parasite. The high prevalence of sarcocysts in bobcat tissues suggested an efficient sylvatic cycle of Sarcocystis spp. in the remote regions of Mississippi State with the bobcat as a relevant intermediate host. Published by Elsevier B.V. C1 [Verma, S. K.; Calero-Bernal, R.; Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Lovallo, M. J.] Penn Game Commiss, Game Mammals Sect, Bur Wildlife Management, Harrisburg, PA 17110 USA. [Sweeny, A. R.; Grigg, M. E.] NIAID, Mol Parasitol Sect, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov FU Department of Employment and Innovation of the Regional Government of Extremadura (Spain) [PO12010]; European Social Fund; Intramural Research Program of the NIH and NIAID FX R. Calero-Bernal is a postdoctoral fellow (Ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund. This study was financially support in part by the Intramural Research Program of the NIH and NIAID. M.E.G. is a scholar of the Canadian Institute for Advanced Research Integrated Microbial Biodiversity Program. NR 20 TC 1 Z9 1 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD SEP 15 PY 2015 VL 212 IS 3-4 BP 422 EP 426 DI 10.1016/j.vetpar.2015.06.007 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CU2LQ UT WOS:000363355400056 PM 26138150 ER PT J AU Wilson, GV Rigby, JR Dabney, SM AF Wilson, Glenn V. Rigby, James R. Dabney, Seth M. TI Soil pipe collapses in a loess pasture of Goodwin Creek watershed, Mississippi: role of soil properties and past land use SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE fragipan; sinkhole; flute hole; internal erosion; pinhole test ID EPHEMERAL GULLY EROSION; PIPING SAPPING EROSION; FLOW-THROUGH; MECHANISMS; CATCHMENT AB Little is known about the association of soil pipe collapse features with soil properties or land use history. Three loess covered catchments in northern Mississippi, USAwere characterized to investigate these relationships. Soil pipe collapses were characterized for their size, type feature and spatial location along with soil properties across the three catchments. Although mapped as the same soil, one of the catchments did not contain pipe collapse features while the other two had 29.4 and 15.4 pipe collapses per hectare. These loess soils contained fragipan layers that are suspected of perching water, thereby initiating the piping processes. Pipe collapses associated with subsurface flow paths were not always consistent with surface topography. The surface layer tended to be non-erodible while layers below, even the upper fragipan layers, were susceptible to erosion by pipeflow. Soil properties of the lowest fragipan layer were highly variable but tended to prevent further downward erosion of soil pipes and thus formed a lower boundary for gullies. Middle to lower landscape positions in one of the piped catchments contained anthropic soils that were highly erodible. These anthropic soils were previously gullies that were filled-in in the 1950s when forested areas, assumed to have been established when land was previously converted from crop to forest land, were converted to pasture. Three decades after this land use change from forest to pasture, pipe collapses became evident. In contrast, the adjacent catchment that does not exhibit pipe collapse features experienced severe sheet and rill erosion prior to the 1930s while in cotton production. The surface horizons above the lower fragipan layer were completely removed during this period, thus the top-soil layer that tends to form a bridge above soil pipes in the more erodible subsoil layers was removed. This study showed that knowledge of soil characteristics or topography alone do not explain the distribution of soil pipe collapses as past land use can play a definitive role. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Wilson, Glenn V.; Rigby, James R.; Dabney, Seth M.] USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Wilson, GV (reprint author), USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, 598 McElroy Dr, Oxford, MS 38655 USA. EM Glenn.Wilson@ars.usda.gov OI Rigby, James/0000-0002-5611-6307 NR 45 TC 4 Z9 5 U1 4 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD SEP 15 PY 2015 VL 40 IS 11 BP 1448 EP 1463 DI 10.1002/esp.3727 PG 16 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CT3CD UT WOS:000362683000003 ER PT J AU Oliveira, PTS Nearing, MA Wendland, E AF Oliveira, Paulo Tarso S. Nearing, Mark A. Wendland, Edson TI Orders of magnitude increase in soil erosion associated with land use change from native to cultivated vegetation in a Brazilian savannah environment SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE deforestation; infiltration; runoff; soil and water conservation; USLE ID COVER-MANAGEMENT FACTOR; LOSS EQUATION; FOREST; VARIABILITY; CERRADO; CONSERVATION; EROSIVITY; RUNOFF; TURKEY; MODEL AB The Brazilian savanna (cerrado) is a large and important economic and environmental region that is experiencing significant loss of its natural landscapes due to pressures of food and energy production, which in turn has caused large increases in soil erosion. However the magnitude of the soil erosion increases in this region is not well understood, in part because scientific studies of surface runoff and soil erosion are scarce or nonexistent in the cerrado as well as in other savannahs of the world. To understand the effects of deforestation we assessed natural rainfall-driven rates of runoff and soil erosion on an undisturbed tropical woodland classified as 'cerrado sensu stricto denso' and bare soil. Results were evaluated and quantified in the context of the cover and management factor (C-factor) of the Universal Soil Loss Equation (USLE). Replicated data on precipitation, runoff, and soil loss on plots (5 x 20m) under undisturbed cerrado and bare soil were collected for 77 erosive storms that occurred over 3 years (2012 through 2014). C-factor was computed annually using values of rainfall erosivity and soil loss rate. We found an average runoff coefficient of similar to 20% for the plots under bare soil and less than 1% under undisturbed cerrado. The mean annual soil losses in the plots under bare soil and cerrado were 12.4 t ha(-1) yr(-1) and 0.1 t ha(-1) yr(-1), respectively. The erosivity-weighted C-factor for the undisturbed cerrado was 0.013. Surface runoff, soil loss and C-factor were greatest in the summer and fall. Our results suggest that shifts in land use from the native to cultivated vegetation result in orders of magnitude increases in soil loss rates. These results provide benchmark values that will be useful to evaluate past and future land use changes using soil erosion models and have significance for undisturbed savanna regions worldwide. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Oliveira, Paulo Tarso S.; Wendland, Edson] Univ Sao Paulo, Dept Hydraul & Sanit Engn, BR-13560970 Sao Carlos, SP, Brazil. [Oliveira, Paulo Tarso S.; Nearing, Mark A.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. RP Oliveira, PTS (reprint author), Univ Sao Paulo, Dept Hydraul & Sanit Engn, CxP 359, BR-13560970 Sao Carlos, SP, Brazil. EM paulotarsoms@gmail.com RI Oliveira, Paulo Tarso/A-4187-2011; Cepid, CeMEAI/J-2417-2015 OI Oliveira, Paulo Tarso/0000-0003-2806-0083; FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo -FAPESP [10/18788-5, 11/14273-3, 12/03764-9]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq [470846/2011-9] FX The authors thank the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo -FAPESP (10/18788-5, 11/14273-3 and 12/03764-9) and the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq (470846/2011-9) for making this study possible. We would like to thank the Arruda Botelho Institute (IAB) and Sao Jose farm that have allowed us to develop this study in the native cerrado vegetation. USDA is an equal opportunity provider and employer. Finally, we appreciate valuable comments and careful reviews from editors, and the anonymous reviewers who helped significantly to improve this manuscript. NR 54 TC 6 Z9 6 U1 4 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD SEP 15 PY 2015 VL 40 IS 11 BP 1524 EP 1532 DI 10.1002/esp.3738 PG 9 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CT3CD UT WOS:000362683000008 ER PT J AU Columbus, DA Steinhoff-Wagner, J Suryawan, A Nguyen, HV Hernandez-Garcia, A Fiorotto, ML Davis, TA AF Columbus, Daniel A. Steinhoff-Wagner, Julia Suryawan, Agus Nguyen, Hanh V. Hernandez-Garcia, Adriana Fiorotto, Marta L. Davis, Teresa A. TI Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE protein synthesis; mammalian target of rapamycin; protein degradation; muscle; newborn ID BIRTH-WEIGHT INFANTS; INITIATION-FACTOR ACTIVATION; AMINO-ACID AVAILABILITY; SKELETAL-MUSCLE; TRANSLATION INITIATION; METABOLIC-RESPONSE; MAMMALIAN TARGET; VISCERAL TISSUES; PRETERM INFANT; YOUNG MEN AB Most low-birth weight infants experience extrauterine growth failure due to reduced nutrient intake as a result of feeding intolerance. The objective of this study was to determine whether prolonged enteral leucine supplementation improves lean growth in neonatal pigs fed a restricted protein diet. Neonatal pigs (n = 14-16/diet, 5 days old, 1.8 +/- 0.3 kg) were fed by gastric catheter a whey-based milk replacement diet with either a high protein (HP) or restricted protein (RP) content or RP supplemented with leucine to the same level as in the HP diet (RPL). Pigs were fed 40 ml.kg body wt(-1).meal(-1) every 4 h for 21 days. Feeding the HP diet resulted in greater total body weight and lean body mass compared with RP-fed pigs (P < 0.05). Masses of the longissimus dorsi muscle, heart, and kidneys were greater in the HP-than RP-fed pigs (P < 0.05). Body weight, lean body mass, and masses of the longissimus dorsi, heart, and kidneys in pigs fed the RPL diet were intermediate to RP-and HP-fed pigs. Protein synthesis and mTOR signaling were increased in all muscles with feeding (P < 0.05); leucine supplementation increased mTOR signaling and protein synthesis rate in the longissimus dorsi (P < 0.05). There was no effect of diet on indices of protein degradation signaling in any tissue (P > 0.05). Thus, when protein intake is chronically restricted, the capacity for leucine supplementation to enhance muscle protein accretion in neonatal pigs that are meal-fed milk protein-based diets is limited. C1 [Columbus, Daniel A.; Steinhoff-Wagner, Julia; Suryawan, Agus; Nguyen, Hanh V.; Hernandez-Garcia, Adriana; Fiorotto, Marta L.; Davis, Teresa A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Davis, TA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates Ave,Rm 9054, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institute of Child Health and Human Development Grant [HD-072891]; USDA National Institute of Food and Agriculture Grant [2013-67015-20438]; National Institute of Arthritis and Musculoskeletal and Skin Diseases Grant [AR-044474]; USDA/ARS Grant [6250-51000-055] FX This work is a publication of the US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine. Financial support for this project was provided by National Institute of Child Health and Human Development Grant HD-072891, USDA National Institute of Food and Agriculture Grant 2013-67015-20438, National Institute of Arthritis and Musculoskeletal and Skin Diseases Grant AR-044474, and USDA/ARS Grant 6250-51000-055. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 59 TC 7 Z9 7 U1 0 U2 7 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD SEP 15 PY 2015 VL 309 IS 6 BP E601 EP E610 DI 10.1152/ajpendo.00089.2015 PG 10 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA CS1II UT WOS:000361817000010 PM 26374843 ER PT J AU Huang, HB Singh, V Qureshi, N AF Huang, Haibo Singh, Vijay Qureshi, Nasib TI Butanol production from food waste: a novel process for producing sustainable energy and reducing environmental pollution SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Butanol; Food waste; Fermentation; Vacuum stripping; Process integration; Energy ID HIGH-SOLIDS FERMENTATION; FED-BATCH FERMENTATION; CLOSTRIDIUM-BEIJERINCKII; ETHANOL-PRODUCTION; SIMULTANEOUS SACCHARIFICATION; ENZYMATIC-HYDROLYSIS; ANAEROBIC-DIGESTION; WHEAT-STRAW; CORN STOVER; RECOVERY AB Background: Waste is currently a major problem in the world, both in the developing and the developed countries. Efficient utilization of food waste for fuel and chemical production can positively influence both the energy and environmental sustainability. This study investigated using food waste to produce acetone, butanol, and ethanol (ABE) by Clostridium beijerinckii P260. Results: In control fermentation, 40.5 g/L of glucose (initial glucose 56.7 g/L) was used to produce 14.2 g/L of ABE with a fermentation productivity and a yield of 0.22 g/L/h and 0.35 g/g, respectively. In a similar fermentation 81 g/L of food waste (containing equivalent glucose of 60.1 g/L) was used as substrate, and the culture produced 18.9 g/L ABE with a high ABE productivity of 0.46 g/L/h and a yield of 0.38 g/g. Fermentation of food waste at higher concentrations (129, 181 and 228 g/L) did not remarkably increase ABE production but resulted in high residual glucose due to the culture butanol inhibition. An integrated vacuum stripping system was designed and applied to recover butanol from the fermentation broth simultaneously to relieve the culture butanol inhibition, thereby allowing the fermentation of food waste at high concentrations. ABE fermentation integrated with vacuum stripping successfully recovered the ABE from the fermentation broth and controlled the ABE concentrations below 10 g/L during fermentation when 129 g/L food waste was used. The ABE productivity with vacuum fermentation was 0.49 g/L/h, which was 109 % higher than the control fermentation (glucose based). More importantly, ABE vacuum recovery and fermentation allowed near-complete utilization of the sugars (similar to 98 %) in the broth. Conclusions: In these studies it was demonstrated that food waste is a superior feedstock for producing butanol using Clostridium beijerinckii. Compared to costly glucose, ABE fermentation of food waste has several advantages including lower feedstock cost, higher productivity, and less residual sugars. C1 [Huang, Haibo; Singh, Vijay] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. [Qureshi, Nasib] ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Singh, V (reprint author), Univ Illinois, Dept Agr & Biol Engn, 1304W Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu; nasib.qureshi@ars.usda.gov NR 45 TC 3 Z9 3 U1 9 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD SEP 15 PY 2015 VL 8 AR 147 DI 10.1186/s13068-015-0332-x PG 12 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA CR8FS UT WOS:000361587100007 PM 26380581 ER PT J AU Witmer, GW Snow, NP Moulton, RS AF Witmer, Gary W. Snow, Nathan P. Moulton, Rachael S. TI Efficacy of potential chemical control compounds for removing invasive American bullfrogs (Rana catesbeiana) SO SPRINGERPLUS LA English DT Article DE Amphibian; Caffeine; Chloroxylenol; Colorado; Integrated pest management; Permethrin; Rotenone; Toxicant ID FROGS; CALIFORNIA; PREDATORS; HAWAII AB Invasive American bullfrogs [Rana catesbeiana (Lithobates catesbeianus)] are outcompeting and predating on native biota and contributing to reductions in biodiversity worldwide. Current methods for controlling American bullfrogs are incapable of stopping their expansion, thus more cost-effective and broadly applicable methods are needed. Although chemical control compounds have been identified as effective for removing other invasive amphibians, none have been tested for American bullfrogs. Our objective was to expand on previous research and test the efficacy of 10 potential chemical control compounds for removing invasive American bullfrogs. After a dermal spray-application of 4 ml, we found 3 compounds (i.e., chloroxylenol, rotenone with permethrin, and caffeine) at 5-10 % concentrations in water were 100 % lethal for adult American bullfrogs. Chloroxylenol and rotenone with permethrin were fast acting with time-to-death < 2 h. This research presents a first-step toward incorporating chemical control as part of integrated pest management strategy for controlling invasive American bullfrogs. Follow-up studies on delivery systems and reducing non-target hazards should ensue with these compounds to confirm their effectiveness and safety for removing invasive American bullfrogs. C1 [Witmer, Gary W.; Snow, Nathan P.; Moulton, Rachael S.] USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Witmer, GW (reprint author), USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM gary.w.witmer@aphis.usda.gov NR 37 TC 0 Z9 0 U1 7 U2 29 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 2193-1801 J9 SPRINGERPLUS JI SpringerPlus PD SEP 15 PY 2015 VL 4 AR 497 DI 10.1186/s40064-015-1319-6 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR4KF UT WOS:000361300200006 PM 26389022 ER PT J AU Loving, CL Osorio, FA Murtaugh, MP Zuckermann, FA AF Loving, Crystal L. Osorio, Fernando A. Murtaugh, Michael P. Zuckermann, Federico A. TI Innate and adaptive immunity against Porcine Reproductive and Respiratory Syndrome Virus SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Review DE PRRSV; Immunology; Antibody; Cell-mediated immunity; Innate immunity ID PLASMACYTOID DENDRITIC CELLS; ANTIBODY-DEPENDENT ENHANCEMENT; SYNDROME PRRS VIRUS; REGULATORY T-CELLS; EXPERIMENTALLY INFECTED-PIGS; DOUBLE-STRANDED-RNA; IN-UTERO INFECTION; EUROPEAN SUBTYPE 1; STRUCTURAL PROTEINS; NEUTRALIZING ANTIBODIES AB Many highly effective vaccines have been produced against viruses whose virulent infection elicits strong and durable protective immunity. In these cases, characterization of immune effector mechanisms and identification of protective epitopes/immunogens has been informative for the development of successful vaccine programs. Diseases in which the immune system does not rapidly clear the acute infection and/or convalescent immunity does not provide highly effective protection against secondary challenge pose a major hurdle for clinicians and scientists. Porcine reproductive and respiratory syndrome virus (PRRSV) falls primarily into this category, though not entirely. PRRSV causes a prolonged infection, though the host eventually clears the virus. Neutralizing antibodies can provide passive protection when present prior to challenge, though infection can be controlled in the absence of detectable neutralizing antibodies. In addition, primed pigs (through natural exposure or vaccination with a modified-live vaccine) show some protection against secondary challenge. While peripheral PRRSV-specific T cell responses have been examined, their direct contribution to antibody-mediated immunity and viral clearance have not been fully elucidated. The innate immune response following PRRSV infection, particularly the antiviral type I interferon response, is meager, but when provided exogenously, IFN-alpha enhances PRRSV immunity and viral control. Overall, the quality of immunity induced by natural PRRSV infection is not ideal for informing vaccine development programs. The epitopes necessary for protection may be identified through natural exposure or modified-live vaccines and subsequently applied to vaccine delivery platforms to accelerate induction of protective immunity following vaccination. Collectively, further work to identify protective B and T cell epitopes and mechanisms by which PRRSV eludes innate immunity will enhance our ability to develop more effective methods to control and eliminate PRRS disease. Published by Elsevier B.V. C1 [Loving, Crystal L.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Osorio, Fernando A.] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE 68583 USA. [Osorio, Fernando A.] Univ Nebraska, Sch Vet & Biomed Sci, Lincoln, NE 68583 USA. [Murtaugh, Michael P.] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. [Zuckermann, Federico A.] Univ Illinois, Coll Vet Med, Dept Pathobiol, Urbana, IL 61801 USA. RP Loving, CL (reprint author), USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. EM crystal.loving@ars.usda.gov FU National Pork Board grant [13-241]; USDA NIFA multistate project [MIN 63-084, MIN63-112]; USDA-AFRI-NIFA [2011-02917, 2013-01035] FX Authors gratefully acknowledge support from the National Pork Board grant #13-241 to CLL, MPM, FAO and FAZ; the USDA NIFA multistate project MIN 63-084 and MIN63-112 to MPM; the USDA-AFRI-NIFA 2011-02917 and 2013-01035 to FAO. NR 173 TC 16 Z9 16 U1 18 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD SEP 15 PY 2015 VL 167 IS 1-2 BP 1 EP 14 DI 10.1016/j.vetimm.2015.07.003 PG 14 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA CR3WS UT WOS:000361264400001 PM 26209116 ER PT J AU Leite, FL Eslabao, LB Pesch, B Bannantine, JP Reinhardt, TA Stabel, JR AF Leite, Fernando L. Eslabao, Livia B. Pesch, Bruce Bannantine, John P. Reinhardt, Timothy A. Stabel, Judith R. TI ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp paratuberculosis SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Mycobacterium avium subsp paratuberculosis; T cell function; ZAP-70; CTLA-4 ID WC1(+) GAMMA-DELTA; PERIPHERAL-BLOOD; JOHNES-DISEASE; EXPRESSION; ACTIVATION; RESPONSES; CATTLE; REGULATOR; SURFACE; LEPROSY AB Paratuberculosis is a chronic intestinal disease of ruminant animals caused by Mycobacterium avium subsp. paratuberculosis (MAP). A hallmark of paratuberculosis is a transition from a cell-mediated Th1 type response to a humoral Th2 response with the progression of disease from a subclinical to clinical state. The objective of this study was to investigate the expression of two crucial molecules in T cell function, ZAP-70 (zeta-chain-associated protein of 70 kDa) and CTLA-4 (cytotoxic T-lymphocyte antigen-4), in cows naturally infected with MAP. Peripheral blood mononuclear cells (PBMCs) isolated from control non-infected cows (n = 5), and cows in subclinical (n = 6) and clinical stages of paratuberculosis (n = 6) were cultured alone (medium only), and with concanavalin A, and a whole cell sonicate of MAP for 24, 72 and 144h to measure the dynamic changes of ZAP-70 and CTLA-4 expression on CD4, CD8, and gamma delta (gamma delta) T cells. Flow cytometry was also performed to measure ZAP-70 phosphorylation to examine proximal T cell receptor signaling in animals of different disease status. The surface expression of CTLA-4 was increased in animals in subclinical stage of infection while levels of ZAP-70 were decreased in CD4+ T cells of both subclinical and clinical animals, indicating a change in T cell phenotype with disease state. Interestingly, proximal T cell receptor signaling was not altered in infected animals. This study demonstrated changes in crucial signaling molecules in animals infected with MAP, thereby elucidating T cell alterations associated with disease progression. Published by Elsevier B.V. C1 [Leite, Fernando L.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. [Eslabao, Livia B.] Univ Fed Pelotas, Pelotas, Brazil. [Pesch, Bruce; Bannantine, John P.; Reinhardt, Timothy A.; Stabel, Judith R.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Stabel, JR (reprint author), USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM judy.stabel@ars.usda.gov OI Bannantine, John/0000-0002-5692-7898 NR 32 TC 4 Z9 4 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD SEP 15 PY 2015 VL 167 IS 1-2 BP 15 EP 21 DI 10.1016/j.vetimm.2015.06.017 PG 7 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA CR3WS UT WOS:000361264400002 PM 26163934 ER PT J AU Liu, ZP Liu, JF Wang, K Li, WH Shelver, WL Li, QX Li, J Xu, T AF Liu, Zhiping Liu, Jianfeng Wang, Kai Li, Wenhui Shelver, Weilin L. Li, Qing X. Li, Ji Xu, Ting TI Selection of phage-displayed peptides for the detection of imidacloprid in water and soil SO ANALYTICAL BIOCHEMISTRY LA English DT Article DE Imidacloprid; Phage-displayed peptide; Phage ELISA; Environmental monitoring; Food safety ID LINKED-IMMUNOSORBENT-ASSAY; IMMUNOASSAYS; SPECIFICITY; SENSITIVITY; MOLECULES; LIGANDS; SURFACE AB Imidacloprid is the most widely used neonicotinoid insecticide in the world and shows widespread environment and human exposures. A phage clone designated L7-1 that selectively binds to imidacloprid was selected from a commercial phage display library containing linear 7-met randomized amino acid residues. Using the clone L7-1, a competitive enzyme-linked immunosorbent assay (ELISA) for imidacloprid was developed. The half-maximum signal inhibition concentration (IC50) and the limit of detection CLOD) of the phage ELISA for imidacloprid were 96 and 2.3 ng ml(-1), respectively. This phage ELISA showed relatively low cross-reactivity with all of the tested compounds structurally similar to imidacloprid, less than 2% with the exception of 6-chloronicotinic acid, a metabolite of imidacloprid that showed 11.5%. The average recoveries of the phage ELISA for imidacloprid in water and soil samples were in the ranges of 74.6 to 86.3% and 72.5 to 93.6%, respectively. The results of the competitive phage ELISA for imidacloprid in the fortified samples agreed well with those of a high-performance liquid chromatography (HPLC) method. The simple phage-displayed peptide technology has been proven to be a convenient and efficient method for the development of an alternative format of ELISA for small molecules. (C) 2015 Elsevier Inc. All rights reserved. C1 [Liu, Zhiping; Liu, Jianfeng; Wang, Kai; Li, Ji; Xu, Ting] China Agr Univ, Coll Resources & Environm Sci, Beijing Key Lab Biodivers & Organ Farming, Beijing 100193, Peoples R China. [Li, Wenhui] Guangdong Inst Cereal Sci Res, Guangzhou 510050, Guangdong, Peoples R China. [Shelver, Weilin L.] USDA ARS, Biosci Res Lab, Fargo, ND 58102 USA. [Li, Qing X.] Univ Hawaii Manoa, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA. RP Xu, T (reprint author), China Agr Univ, Coll Resources & Environm Sci, Beijing Key Lab Biodivers & Organ Farming, Beijing 100193, Peoples R China. EM xuting@cau.edu.cn FU PhD Programs Foundation of Ministry of Education of China [20130008110019]; Special Project on the Integration of Industry, Education, and Research of Guangdong Province [2012B091100281]; Major Project on the Collaborative Innovation of Industry, Education, and Research of Guangzhou [2060404]; U.S. National Institute of Occupational Safety and Health [2U54OH007550] FX This work was supported in part by the PhD Programs Foundation of Ministry of Education of China (20130008110019), the Special Project on the Integration of Industry, Education, and Research of Guangdong Province (2012B091100281), the Major Project on the Collaborative Innovation of Industry, Education, and Research of Guangzhou (2060404), and the U.S. National Institute of Occupational Safety and Health (2U54OH007550). NR 34 TC 6 Z9 6 U1 1 U2 28 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 EI 1096-0309 J9 ANAL BIOCHEM JI Anal. Biochem. PD SEP 15 PY 2015 VL 485 BP 28 EP 33 DI 10.1016/j.ab.2015.05.014 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CO0BK UT WOS:000358814900005 PM 26048649 ER PT J AU Nowak, V Rittenschober, D Exler, J Charrondiere, UR AF Nowak, Verena Rittenschober, Doris Exler, Jacob Charrondiere, U. Ruth TI Untitled Reply SO FOOD CHEMISTRY LA English DT Letter ID FATTY-ACIDS; COMPREHENSIVE EVALUATION; PRODUCTS; FOODS C1 [Nowak, Verena; Rittenschober, Doris; Charrondiere, U. Ruth] ESN, FAO, I-00153 Rome, Italy. [Exler, Jacob] ARS, USDA, Beltsville Human Nutr Res Ctr, Nutrient Data Lab, Beltsville, MD USA. RP Nowak, V (reprint author), ESN, FAO, Viale Terme Caracalla 1, I-00153 Rome, Italy. EM vnowak@gmx.at OI Nowak, Verena/0000-0003-2523-579X NR 10 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD SEP 15 PY 2015 VL 183 BP 306 EP 306 DI 10.1016/j.foodchem.2015.02.029 PG 1 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CG8ZD UT WOS:000353603300039 PM 25863640 ER PT J AU Astudillo-Reyes, C Fernandez, AC Cichy, KA AF Astudillo-Reyes, Carolina Fernandez, Andrea C. Cichy, Karen A. TI Transcriptome Characterization of Developing Bean (Phaseolus vulgaris L.) Pods from Two Genotypes with Contrasting Seed Zinc Concentrations SO PLOS ONE LA English DT Article ID ARABIDOPSIS-THALIANA; MEDICAGO-TRUNCATULA; GENETIC DIVERSITY; IRON-DEFICIENCY; RNA-SEQ; PROTEIN; FAMILY; NICOTIANAMINE; EXPRESSION; GROWTH AB Dry bean (Phaseolus vulgaris L.) seeds are a rich source of dietary zinc, especially for people consuming plant-based diets. Within P. vulgaris there is at least two-fold variation in seed Zn concentration. Genetic studies have revealed seed Zn differences to be controlled by a single gene in two closely related navy bean genotypes, Albion and Voyager. In this study, these two genotypes were grown under controlled fertilization conditions and the Zn concentration of various plant parts was determined. The two genotypes had similar levels of Zn in their leaves and pods but Voyager had 52% more Zn in its seeds than Albion. RNA was sequenced from developing pods of both genotypes. Transcriptome analysis of these genotypes identified 27,198 genes in the developing bean pods, representing 86% of the genes in the P. vulgaris genome (v 1.0 DOE-JGI and USDA- NIFA). Expression was detected in 18,438 genes. A relatively small number of genes (381) were differentially expressed between Albion and Voyager. Differentially expressed genes included three genes potentially involved in Zn transport, including zinc-regulated transporter, iron regulated transporter like (ZIP), zinc-induced facilitator (ZIF) and heavy metal associated (HMA) family genes. In addition 12,118 SNPs were identified between the two genotypes. Of the gene families related to Zn and/or Fe transport, eleven genes were found to contain SNPs between Albion and Voyager. C1 [Astudillo-Reyes, Carolina; Fernandez, Andrea C.; Cichy, Karen A.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Cichy, Karen A.] USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48823 USA. RP Cichy, KA (reprint author), USDA ARS, Sugarbeet & Bean Res Unit, 1066 Bogue St, E Lansing, MI 48823 USA. EM Karen.Cichy@ars.usda.gov FU USDA-ARS FX The authors received USDA-ARS funding for this work. NR 61 TC 0 Z9 0 U1 3 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 14 PY 2015 VL 10 IS 9 AR e0137157 DI 10.1371/journal.pone.0137157 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8KS UT WOS:000361601100052 PM 26367119 ER PT J AU Bedre, R Rajasekaran, K Mangu, VR Timm, LES Bhatnagar, D Baisakh, N AF Bedre, Renesh Rajasekaran, Kanniah Mangu, Venkata Ramanarao Timm, Luis Eduardo Sanchez Bhatnagar, Deepak Baisakh, Niranjan TI Genome-Wide Transcriptome Analysis of Cotton (Gossypium hirsutum L.) Identifies Candidate Gene Signatures in Response to Aflatoxin Producing Fungus Aspergillus flavus SO PLOS ONE LA English DT Article ID PLANT DEFENSE GENES; STRESS RESPONSES; CHITINASE GENE; RNA-SEQ; NICOTIANA-BENTHAMIANA; SIGNALING NETWORKS; BIOLOGICAL-CONTROL; NITRIC-OXIDE; RESISTANCE; BIOSYNTHESIS AB Aflatoxins are toxic and potent carcinogenic metabolites produced from the fungi Aspergillus flavus and A. parasiticus. Aflatoxins can contaminate cottonseed under conducive pre-harvest and postharvest conditions. United States federal regulations restrict the use of aflatoxin contaminated cottonseed at >20 ppb for animal feed. Several strategies have been proposed for controlling aflatoxin contamination, and much success has been achieved by the application of an atoxigenic strain of A. flavus in cotton, peanut and maize fields. Development of cultivars resistant to aflatoxin through overexpression of resistance associated genes and/ or knocking down aflatoxin biosynthesis of A. flavus will be an effective strategy for controlling aflatoxin contamination in cotton. In this study, genome-wide transcriptome profiling was performed to identify differentially expressed genes in response to infection with both toxigenic and atoxigenic strains of A. flavus on cotton (Gossypium hirsutum L.) pericarp and seed. The genes involved in antifungal response, oxidative burst, transcription factors, defense signaling pathways and stress response were highly differentially expressed in pericarp and seed tissues in response to A. flavus infection. The cell-wall modifying genes and genes involved in the production of antimicrobial substances were more active in pericarp as compared to seed. The genes involved in auxin and cytokinin signaling were also induced. Most of the genes involved in defense response in cotton were highly induced in pericarp than in seed. The global gene expression analysis in response to fungal invasion in cotton will serve as a source for identifying biomarkers for breeding, potential candidate genes for transgenic manipulation, and will help in understanding complex plant-fungal interaction for future downstream research. C1 [Bedre, Renesh; Mangu, Venkata Ramanarao; Timm, Luis Eduardo Sanchez; Baisakh, Niranjan] Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. [Rajasekaran, Kanniah; Bhatnagar, Deepak] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Baisakh, N (reprint author), Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. EM nbaisakh@agcenter.lsu.edu FU United States Department of Agriculture-National Institute of Food and Agriculture (SCA) [58-6435-0-529] FX Funded by United States Department of Agriculture-National Institute of Food and Agriculture (SCA# 58-6435-0-529). NR 75 TC 3 Z9 3 U1 1 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 14 PY 2015 VL 10 IS 9 AR e0138025 DI 10.1371/journal.pone.0138025 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8KS UT WOS:000361601100204 PM 26366857 ER PT J AU Dhanapal, AP Ray, JD Singh, SK Hoyos-Villegas, V Smith, JR Purcell, LC King, CA Fritschi, FB AF Dhanapal, Arun Prabhu Ray, Jeffery D. Singh, Shardendu K. Hoyos-Villegas, Valerio Smith, James R. Purcell, Larry C. King, C. Andy Fritschi, Felix B. TI Association Mapping of Total Carotenoids in Diverse Soybean Genotypes Based on Leaf Extracts and High-Throughput Canopy Spectral Reflectance Measurements SO PLOS ONE LA English DT Article ID ABSCISIC-ACID BIOSYNTHESIS; GENOME-WIDE ASSOCIATION; HIGH LIGHT STRESS; XANTHOPHYLL CYCLE; CHLOROPHYLL CONCENTRATION; WAVELET DECOMPOSITION; POPULATION-STRUCTURE; OPTICAL-PROPERTIES; NITROGEN-CONTENT; BETA-CAROTENE AB Carotenoids are organic pigments that are produced predominantly by photosynthetic organisms and provide antioxidant activity to a wide variety of plants, animals, bacteria, and fungi. The carotenoid biosynthetic pathway is highly conserved in plants and occurs mostly in chromoplasts and chloroplasts. Leaf carotenoids play important photoprotective roles and targeted selection for leaf carotenoids may offer avenues to improve abiotic stress tolerance. A collection of 332 soybean [Glycine max (L.) Merr.] genotypes was grown in two years and total leaf carotenoid content was determined using three different methods. The first method was based on extraction and spectrophotometric determination of carotenoid content (eCaro) in leaf tissue, whereas the other two methods were derived from high-throughput canopy spectral reflectance measurements using wavelet transformed reflectance spectra (tCaro) and a spectral reflectance index (iCaro). An association mapping approach was employed using 31,253 single nucleotide polymorphisms (SNPs) to identify SNPs associated with total carotenoid content using a mixed linear model based on data from two growing seasons. A total of 28 SNPs showed a significant association with total carotenoid content in at least one of the three approaches. These 28 SNPs likely tagged 14 putative loci for carotenoid content. Six putative loci were identified using eCaro, five loci with tCaro, and nine loci with iCaro. Three of these putative loci were detected by all three carotenoid determination methods. All but four putative loci were located near a known carotenoid-related gene. These results showed that carotenoid markers can be identified in soybean using extract-based as well as by high-throughput canopy spectral reflectance-based approaches, demonstrating the utility of field-based canopy spectral reflectance phenotypes for association mapping. C1 [Dhanapal, Arun Prabhu; Fritschi, Felix B.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Ray, Jeffery D.; Smith, James R.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Singh, Shardendu K.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD USA. [Hoyos-Villegas, Valerio] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Purcell, Larry C.; King, C. Andy] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. RP Fritschi, FB (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM fritschif@missouri.edu OI Dhanapal, Arun Prabhu/0000-0002-9686-7260 FU United States Department of Agriculture-Agriculture Research Service (USDA-ARS) [6066-21220-012-00D]; United Soybean Board project [9274, 1274] FX This work was supported by United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project number 6066-21220-012-00D and United Soybean Board project numbers 9274 and 1274. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 82 TC 1 Z9 1 U1 1 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 14 PY 2015 VL 10 IS 9 AR e0137213 DI 10.1371/journal.pone.0137213 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8KS UT WOS:000361601100058 PM 26368323 ER PT J AU Li, YK Carrillo, JA Ding, Y He, YH Zhao, CP Liu, JN Liu, GE Zan, LS Song, JZ AF Li, Yaokun Carrillo, Jose A. Ding, Yi He, Yanghua Zhao, Chunping Liu, Jianan Liu, George E. Zan, Linsen Song, Jiuzhou TI Transcriptomic Profiling of Spleen in Grass-Fed and Grain-Fed Angus Cattle SO PLOS ONE LA English DT Article ID DAIRY BEEF STEERS; HYPERCHOLESTEROLEMIC MEN; ANTIOXIDANT STATUS; IMMUNE-RESPONSE; VITAMIN-E; PASTURE; DIET; MEAT; IDENTIFICATION; CHOLESTEROL AB The grass-fed cattle obtain nutrients directly from pastures containing limited assimilable energy but abundant amount of fiber; by contrast, grain-fed steers receive a diet that is comprised mainly of grains and serves as an efficient source of high-digestible energy. Besides energy, these two types of diet differ in a large number of nutritional components. Additionally, animals maintained on rich-energy regimen are more likely to develop metabolic disorders and infectious diseases than pasture raised individuals. Thus, we hypothesize that spleen-a relevant immune organ-may function differently under disparate regimes. The objective of this study was to find the differentially expressed genes in the spleen of grass-fed and grain-fed steers, and furtherly explore the potential involved biopathways. Through RNA sequencing (RNA-Seq), we detected 123 differentially expressed genes. Based on these genes, we performed an Ingenuity Pathway Analysis (IPA) and identified 9 significant molecular networks and 13 enriched biological pathways. Two of the pathways, Nur77 signaling in T lymphocytes and calcium-induced T lymphocyte apoptosis which are immune related, contain a pair of genes HLA-DRA and NR4A1 with dramatically altered expression level. Collectively, our results provided valuable insights into understanding the molecular mechanism of spleen under varied feeding regimens. C1 [Li, Yaokun; Zhao, Chunping; Zan, Linsen] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. [Carrillo, Jose A.; Ding, Yi; He, Yanghua; Liu, Jianan; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Liu, George E.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Zan, LS (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. EM zanlinsen@163.com; songj88@umd.edu FU China Scholarship Council (CSC); Maryland Agricultural Experiment Station (MAES); Jorgensen Endowment Funds FX The work was supported by China Scholarship Council (CSC), Maryland Agricultural Experiment Station (MAES) and Jorgensen Endowment Funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 2 Z9 2 U1 0 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 14 PY 2015 VL 10 IS 9 AR e0135670 DI 10.1371/journal.pone.0135670 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8KS UT WOS:000361601100012 PM 26367387 ER PT J AU Nyasembe, VO Cheseto, X Kaplan, F Foster, WA Teal, PEA Tumlinson, JH Borgemeister, C Torto, B AF Nyasembe, Vincent O. Cheseto, Xavier Kaplan, Fatma Foster, Woodbridge A. Teal, Peter E. A. Tumlinson, James H. Borgemeister, Christian Torto, Baldwyn TI The Invasive American Weed Parthenium hysterophorus Can Negatively Impact Malaria Control in Africa SO PLOS ONE LA English DT Article ID VECTOR ANOPHELES-GAMBIAE; CLIMATE-CHANGE; WESTERN KENYA; BIDENS-PILOSA; FLORAL NECTAR; PLANTS; SURVIVAL; CULICIDAE; MOSQUITOS; DIPTERA AB The direct negative effects of invasive plant species on agriculture and biodiversity are well known, but their indirect effects on human health, and particularly their interactions with disease-transmitting vectors, remains poorly explored. This study sought to investigate the impact of the invasive Neotropical weed Parthenium hysterophorus and its toxins on the survival and energy reserves of the malaria vector Anopheles gambiae. In this study, we compared the fitness of An. gambiae fed on three differentially attractive mosquito host plants and their major toxins; the highly aggressive invasive Neotropical weed Parthenium hysterophorus (Asteraceae) in East Africa and two other adapted weeds, Ricinus communis (Euphorbiaceae) and Bidens pilosa (Asteraceae). Our results showed that female An. gambiae fitness varied with host plants as females survived better and accumulated substantial energy reserves when fed on P. hysterophorus and R. communis compared to B. pilosa. Females tolerated parthenin and 1-phenylhepta-1, 3, 5-triyne, the toxins produced by P. hysterophorus and B. pilosa, respectively, but not ricinine produced by R. communis. Given that invasive plants like P. hysterophorus can suppress or even replace less competitive species that might be less suitable host-plants for arthropod disease vectors, the spread of invasive plants could lead to higher disease transmission. Parthenium hysterophorus represents a possible indirect effect of invasive plants on human health, which underpins the need to include an additional health dimension in risk-analysis modelling for invasive plants. C1 [Nyasembe, Vincent O.; Cheseto, Xavier; Borgemeister, Christian; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol, Nairobi, Kenya. [Kaplan, Fatma; Teal, Peter E. A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Kaplan, Fatma] Kaplan Schiller Res LLC, Gainesville, FL 32604 USA. [Foster, Woodbridge A.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. [Tumlinson, James H.] Penn State Univ, Dept Entomol, Ctr Chem Ecol, University Pk, PA 16802 USA. [Borgemeister, Christian] Univ Bonn, Ctr Dev Res ZEF, D-53113 Bonn, Germany. RP Torto, B (reprint author), Int Ctr Insect Physiol & Ecol, Box 30772, Nairobi, Kenya. EM btorto@icipe.org FU USDA/ARS - Center for Medical, Agricultural, and Veterinary Entomology; U.S. National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID) [RO1A1077722] FX This study was funded in part by USDA/ARS - Center for Medical, Agricultural, and Veterinary Entomology; and by the U.S. National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID) grant RO1A1077722. NR 55 TC 4 Z9 4 U1 7 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 14 PY 2015 VL 10 IS 9 AR e0137836 DI 10.1371/journal.pone.0137836 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8KS UT WOS:000361601100150 PM 26367123 ER PT J AU You, L Simmons, DR Bateman, CC Short, DPG Kasson, MT Rabaglia, RJ Hulcr, J AF You, Li Simmons, David Rabern Bateman, Craig C. Short, Dylan P. G. Kasson, Matthew T. Rabaglia, Robert J. Hulcr, Jiri TI New Fungus-Insect Symbiosis: Culturing, Molecular, and Histological Methods Determine Saprophytic Polyporales Mutualists of Ambrosiodmus Ambrosia Beetles SO PLOS ONE LA English DT Article ID RIBOSOMAL DNA; COLEOPTERA; SCOLYTIDAE; EVOLUTION; IDENTIFICATION; AGRICULTURE; EUWALLACEA; DIVERSITY AB Ambrosia symbiosis is an obligate, farming-like mutualism between wood-boring beetles and fungi. It evolved at least 11 times and includes many notorious invasive pests. All ambrosia beetles studied to date cultivate ascomycotan fungi: early colonizers of recently killed trees with poor wood digestion. Beetles in the widespread genus Ambrosiodmus, however, colonize decayed wood. We characterized the mycosymbionts of three Ambrosiodmus species using quantitative culturing, high-throughput metabarcoding, and histology. We determined the fungi to be within the Polyporales, closely related to Flavodon flavus. Culture-independent sequencing of Ambrosiodmus minor mycangia revealed a single operational taxonomic unit identical to the sequences from the cultured Flavodon. Histological sectioning confirmed that Ambrosiodmus possessed preoral mycangia containing dimitic hyphae similar to cultured F. cf. flavus. The Ambrosiodmus-Flavodon symbiosis is unique in several aspects: it is the first reported association between an ambrosia beetle and a basidiomycotan fungus; the mycosymbiont grows as hyphae in the mycangia, not as budding pseudo-mycelium; and the mycosymbiont is a white-rot saprophyte rather than an early colonizer: a previously undocumented wood borer niche. Few fungi are capable of turning rotten wood into complete animal nutrition. Several thousand beetle-fungus symbioses remain unstudied and promise unknown and unexpected mycological diversity and enzymatic innovations. C1 [You, Li; Simmons, David Rabern; Hulcr, Jiri] Univ Florida, Inst Food & Agr Sci, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Bateman, Craig C.; Hulcr, Jiri] Univ Florida, Inst Food & Agr Sci, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Short, Dylan P. G.; Kasson, Matthew T.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Rabaglia, Robert J.] ARS, Forest Hlth Protect State & Private Forestry, USDA, Washington, DC USA. RP Hulcr, J (reprint author), Univ Florida, Inst Food & Agr Sci, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. EM hulcr@ufl.edu FU USDA-FS-SRS [14-CA-11330130-032]; USDA-FS-FHP [12-CA-11420004-042]; USDA [14-8130-0377-CA]; NSF [DEB 1256968]; West Virginia Agricultural Experiment Station; University of Florida Opportunity Seed fund FX This research was funded by USDA-FS-SRS Coop agreement 14-CA-11330130-032, USDA-FS-FHP Coop agreement 12-CA-11420004-042, USDA Farm Bill agreement 14-8130-0377-CA, NSF DEB 1256968, the West Virginia Agricultural Experiment Station, and a University of Florida Opportunity Seed fund. NR 49 TC 2 Z9 2 U1 4 U2 64 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 14 PY 2015 VL 10 IS 9 AR e0137689 DI 10.1371/journal.pone.0137689 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR8KS UT WOS:000361601100109 ER PT J AU Chen, J Liu, ZS Li, K Huang, JR Nie, XA Zhou, YH AF Chen, Jie Liu, Zengshe Li, Ke Huang, Jinrui Nie, Xiaoan Zhou, Yonghong TI Synthesis and application of a natural plasticizer based on cardanol for poly(vinyl chloride) SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE plasticizer; poly(vinyl chloride); recycling resins ID FATTY-ACID; THERMAL-DEGRADATION; RENEWABLE RESOURCE; PVC PLASTISOLS; OIL; FILMS; STABILIZATION; DEHP; DEHYDROCHLORINATION; POLYVINYL-CHLORIDE) AB A natural plasticizer with multifunctional groups, similar in structure to phthalates, cardanol derivatives glycidyl ether (CGE) was synthesized from cardanol by a two-step modification process and characterized by FT-IR, (HNMR)-H-1, and (CNMR)-C-13. The resulting product was incorporated to PVC (CGE/PVC), and plasticizing effect was compared with PVC incorporated with two kinds of commercial phthalate ester plasticizers bis (2-ethylhexyl) benzene-1,4-dicarboxylate (DOTP) and diisononyl phthalate (DINP). Dynamic mechanical analysis and mechanical properties testing of the plasticized PVC samples were performed in order to evaluate their flexibility, compatibility, and plasticizing efficiency. SEM was employed to produce fractured surface morphology. Thermogravimetric analysis and discoloration tests were used to characterize the thermal stabilities. Dynamic stability analysis was used to test the processability of formulations. Compared with DOTP and DINP plasticized samples, CGE/PVC has a maximum decrease of 9.27% in glass transition temperature (T-g), a maximum increase of 17.6% in the elongation at break, and a maximum increase of 31.59 degrees C and 25.31 min in 50% weight loss (T50) and dynamic stability time, respectively. The obtained CGE also has slightly lower volatility resistance and higher exudation resistance than that of DOTP and DINP. (C) 2015 Wiley Periodicals, Inc. C1 [Chen, Jie; Li, Ke; Huang, Jinrui; Nie, Xiaoan; Zhou, Yonghong] CAF, Inst Chem Ind Forestry Prod, Natl Engn Lab Biomass Chem Utilizat, Key Lab Biomass Energy & Mat, Nanjing, Jiangsu, Peoples R China. [Chen, Jie; Nie, Xiaoan; Zhou, Yonghong] CAF, Inst New Technol Forestry, Beijing, Peoples R China. [Liu, Zengshe] USDA ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL USA. RP Nie, XA (reprint author), CAF, Inst Chem Ind Forestry Prod, Natl Engn Lab Biomass Chem Utilizat, Key Lab Biomass Energy & Mat, Nanjing, Jiangsu, Peoples R China. EM chenjie_hi@126.com; niexiaoan@126.com FU CAF [CAFINT2014C12]; National "Twelfth Five-Year" Plan for Science & Technology Support [2014BAD02B02] FX The authors are grateful for the financial support from Basic research funding earmarked for the national commonwealth research institutes, CAF (grant number: CAFINT2014C12), National "Twelfth Five-Year" Plan for Science & Technology Support (grant number: 2014BAD02B02). NR 51 TC 5 Z9 6 U1 10 U2 74 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD SEP 12 PY 2015 VL 132 IS 35 AR 42465 DI 10.1002/app.42465 PG 9 WC Polymer Science SC Polymer Science GA CL2XY UT WOS:000356812400004 ER PT J AU Holland, JB AF Holland, James B. TI MAGIC maize: a new resource for plant genetics SO GENOME BIOLOGY LA English DT Editorial Material AB A multiparent advanced-generation intercross population of maize has been developed to help plant geneticists identify sequence variants affecting important agricultural traits. C1 N Carolina State Univ, USDA ARS, Dept Crop Sci, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Holland, JB (reprint author), N Carolina State Univ, USDA ARS, Dept Crop Sci, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM james_holland@ncsu.edu NR 10 TC 1 Z9 1 U1 2 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1465-6906 EI 1474-760X J9 GENOME BIOL JI Genome Biol. PD SEP 11 PY 2015 VL 16 AR 163 DI 10.1186/s13059-015-0713-2 PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CR0QN UT WOS:000361028300001 PM 26357905 ER PT J AU Pitino, M Armstrong, CM Duan, YP AF Pitino, Marco Armstrong, Cheryl M. Duan, Yongping TI Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses SO HORTICULTURE RESEARCH LA English DT Article ID AXONOPODIS PV. CITRI; SYSTEMIC ACQUIRED-RESISTANCE; XANTHOMONAS-CITRI; INNATE IMMUNITY; SUBSP CITRI; HOST-RANGE; ZYGOTIC SEEDLINGS; DEFENSE RESPONSE; BACTERIAL SPOT; SSR MARKERS AB Citrus canker, caused by the bacterial pathogen Xanthomonas citri ssp. citri (Xcc), has been attributed to millions of dollars in loss or damage to commercial citrus crops in subtropical production areas of the world. Since identification of resistant plants is one of the most effective methods of disease management, the ability to screen for resistant seedlings plays a key role in the production of a long-term solution to canker. Here, an inverse correlation between reactive oxygen species (ROS) production by the plant and the ability of Xcc to grow and form lesions on infected plants is reported. Based on this information, a novel screening method that can rapidly identify citrus seedlings that are less susceptible to early infection by Xcc was devised by measuring ROS accumulation triggered by a 22-amino acid sequence of the conserved N-terminal part of flagellin (flg22) from X. citri ssp. citri (Xcc-flg22). In addition to limiting disease symptoms, ROS production was also correlated with the expression of basal defense-related genes such as the pattern recognition receptors LRR8 and FLS2, the leucine-rich repeat receptor-like protein RLP12, and the defense-related gene PR1, indicating an important role for pathogen-associated molecular pattern-triggered immunity (PTI) in determining resistance to citrus canker. Moreover, the differential expression patterns observed amongst the citrus seedlings demonstrated the existence of genetic variations in the PTI response among citrus species/varieties. C1 [Pitino, Marco; Armstrong, Cheryl M.; Duan, Yongping] USDA ARS, Ft Pierce, FL 34945 USA. RP Duan, YP (reprint author), USDA ARS, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM Yongping.Duan@ars.usda.gov NR 66 TC 4 Z9 4 U1 3 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD SEP 9 PY 2015 VL 2 AR 15042 DI 10.1038/hortres.2015.42 PG 8 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA CR8PK UT WOS:000361616000001 PM 26504581 ER PT J AU Su, HH Cheng, YM Wang, ZY Li, Z Stanley, D Yang, YZ AF Su, Honghua Cheng, Yuming Wang, Zhongyang Li, Zhong Stanley, David Yang, Yizhong TI Silk Gland Gene Expression during Larval-Pupal Transition in the Cotton Leaf Roller Sylepta derogata (Lepidoptera: Pyralidae) SO PLOS ONE LA English DT Article ID PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN; BOMBYX-MORI; ECDYSONE-OXIDASE; CLONING; CELLS AB The cotton leaf roller, Sylepta derogata, is a silk-producing insect pest. While young larvae feed on the underside of leaves, the older ones roll cotton leaves and feed on the leaf edges, which defoliates cotton plants. The larvae produce silk to stabilize the rolled leaf and to balloon from used to new leaves. Despite the significance of silk in the biology of pest insect species, there is virtually no information on the genes involved in their silk production. This is a substantial knowledge gap because some of these genes may be valuable targets for developing molecular pest management technologies. We addressed the gap by posing the hypothesis that silk gland gene expression changes during the transition from larvae to pupae. We tested our hypothesis using RNA-seq to investigate changes in silk gland gene expression at three developmental stages, 5th instar larvae (silk producing; 15,445,926 clean reads), prepupae (reduced silk producing; 13,758,154) and pupae (beyond silk producing; 16,787,792). We recorded 60,298 unigenes and mapped 50,158 (larvae), 48,415 (prepupae) and 46,623 (pupae) of them to the NCBI database. Most differentially expressed genes in the 5th instar larvae/prepupae libraries were relevant to nucleotide synthesis and maintenance of silk gland function. We identified down-regulated transcriptional factors and several genes involved in silk formation in the three libraries and verified the expression pattern of eight genes by qPCR. The developmental-and tissue-specific expression patterns of the fibroin light chain gene showed it was highly expressed during the larval silk-producing stage. We recorded highest expression of this gene in the larval silk gland, compared to other tissues, including midgut, hindgut, epidermis, Malpighian tubes, hemolymph and fat body. These data are a genetic resource to guide selection of key genes that may be targeted for in planta and other gene-silencing technologies for sustainable cotton agriculture. C1 [Su, Honghua; Cheng, Yuming; Li, Zhong; Yang, Yizhong] Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. [Wang, Zhongyang] Yangzhou Termite Control Ctr, Yangzhou 225001, Jiangsu, Peoples R China. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Yang, YZ (reprint author), Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. EM yzyang@yzu.edu.cn FU major science and technology project - Ministry of Agriculture of the People's Republic of China [2014ZX08012004-007]; Yangzhou Termite Control Center FX This research was supported by a major science and technology project to create new crop varieties using gene transfer technology (Biosafety Monitoring Technique of Genetically Modified Organisms: 2014ZX08012004-007) funded by Ministry of Agriculture of the People's Republic of China. Co-author Zhongyang Wang is employed by Yangzhou Termite Control Center. Yangzhou Termite Control Center provided support in the form of salary for author Zhongyang Wang, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of this author is articulated in the 'author contributions' section. NR 28 TC 0 Z9 0 U1 5 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 9 PY 2015 VL 10 IS 9 AR e0136868 DI 10.1371/journal.pone.0136868 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ9KG UT WOS:000360932800051 PM 26352931 ER PT J AU Chen, XL Norrbom, A Freidberg, A Chesters, D Islam, MS Zhu, CD AF Chen, Xiao-Lin Norrbom, Allen Freidberg, Amnon Chesters, Douglas Islam, Md Sajedul Zhu, Chao-Dong TI A systematic study of Ichneumonosoma de Meijere, Pelmatops Enderlein, Pseudopelmatops Shiraki and Soita Walker (Diptera: Tephritidae) SO ZOOTAXA LA English DT Article DE Tephritidae; Ichneumonosoma; Pelmatops; Pseudopelmatops; Soita; phylogeny; new species ID FRUIT-FLIES AB Four fruit fly genera, Ichneumonosoma de Meijere, Pelmatops Enderlein, Pseudopelmatops Shiraki and Soita Walker, were studied and 19 species are recognized. Three new species, Soita infuscata Chen & Norrbom, Ichneumonosoma quadripunctata Chen & Freidberg, and I. triangularis Chen & Norrbom are described and illustrated. Ichneumonosoma and Soita are revised, and keys to all the species are provided. Ichneumonosoma imitans (de Meijere) is newly recorded from Thailand. One new synonym is established: Soita Walker = Xaniosternum Enderlein, and Xaniosternum ophioneum Enderlein is moved from Xaniosternum to Soita (n. comb.). In addition, new morphological, geographic and biological information for two stalk-eyed fruit fly genera, Pelmatops and Pseudopelmatops, are provided. Pelmatops fukienensis Zia & Chen is newly recorded from Burma, Pelmetops ichneumoneus (Westwood) is newly recorded from Thailand and Burma, Pseudopelmatops angustifasciatus Zia & Chen is newly recorded from Vietnam, and the male of P. angustifasciatusis described and illustrated for the first time. The morphology of the compound eye and occipital protuberance of Pelmatops and Pseudopelmatops is described and illustrated for the first time. A cladistic analysis based on morphological characters of adults, a partial molecular analysis using the nuclear 28S rDNA (28S) and the mitochondrial cytochrome c oxidase I (COI) genes and a combined dataset were conducted to reconstruct the phylogeny of the four genera and their species. The results showed good support for monophyly of each of the four genera and the clade of the stalk-eyed fruit flies (Pelmatops + Pseudopelmatops). However, relationships of the stalk-eyed fruit flies with Soita and Ichneumonosomaare not clearly resolved, with the morphological analysis indicating that Ichneumonosoma is the sister group of the stalkeyed fruit flies, but the 28S analysis and the combined analysis group Soita closer to the stalk-eyed fruit flies. Regarding relationships amongst congeners, Pelmatops was well resolved; Ichneumonosoma and Soita were partly resolved, and Pseudopelmatops was unresolved. In addition, a hypothesis about the biology of Pseudopelmatops and its relationship with Sesiidae (Lepidoptera) is discussed. C1 [Chen, Xiao-Lin; Chesters, Douglas; Zhu, Chao-Dong] Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China. [Norrbom, Allen] ARS, Systemat Entomol Lab, USDA, Washington, DC USA. [Freidberg, Amnon] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel. [Islam, Md Sajedul] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. RP Chen, XL (reprint author), Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing 100101, Peoples R China. EM xlchen@ioz.ac.cn; allen.norrbom@ars.usda.gov; afdipter@post.tau.ac.il; dchesters@ioz.ac.cn; M.sajed@gmail.com; zhucd@ioz.ac.cn FU National Natural Science Foundation of China [31372169, 61273289]; Program of Ministry of Science and Technology of the Republic of China [2012FY111100]; Special Fund for Entry-Exit Inspections and Quarantine [201210079, 201010042]; K. C. Wong Education Foundation, Hong Kong; National Science Fund for Fostering Talents in Basic Research [NSFC-J0630964/J0109]; General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China [2014IK17] FX We sincerely thank the following scientists, curators and institutions who kindly loaned or provided specimens for study: David R. Britton (AMS); Ding Yang and Xin-li Wang (BAU); Neal Evenhuis, Shepherd Myers and Keith Arakaki (BPBM); Charles E. Griswold (CAS); Masahiro Sueyoshi (FFPRI); Kathleen R. Methven (INHS); Jie Wu and Wei-bing Zhu (IPPE); Ying-lun Wang (NAFU); Rikio Matsumoto (OMNH); Herman de Jong (ZMAN) and Joachim Ziegler (ZMHB). We thank David Hancock (Cairns, Australia) who checked some Pelmatops and Pseudopelmatops specimens in the BMNH for this study and reviewed the draft of this paper, Valery A. Korneyev (I.I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine) who reviewed this paper, Eduard Jendek (Ottawa Plant Laboratory, Canadian Food Inspection Agency) who provided photos and videos of Pelmetops tanliangi, Sorasak Nak-iam (Naresuan University, Thailand) who provided photos of Pelmeyops ichneumoneus, Chun-sheng Wu (IZCAS), Rong Li (Kunming Institute of Botany, Chinese Academy of Sciences, China) and Ming-shui Zhao (Tianmu Mountain Nature Reserve) for identifications of Sesiidae and plants, Yong Wang (IZCAS) and Lucrecia Rodriguez (USNM) who helped with imaging, and Xue-lin Chen for assistance in preparing the plates. Martin Hauser, Bruce A. McPheron, Nobuhiko Kagesawa, Bernhard Merz, Feng Yuan, Hui Dong, Liang Tang, Mei-cai Wei, Ting-ting Zhang, Xiu-shuai Yang, Xing-jing Wang, Li-bing Zhou, Wang-zhong He, Yong-hua Bai and Yu-liang Deng helped to find specimens, provide valuable information or give other helps. Elizabeth Morgulis (TAUI) reviewed an early version of the molecular part of this paper. We thank Tianmu Mountain Nature Reserve (Mingshui Zhao) and Naban River Nature Reserve (Guanghong Cao) for providing sites for study. This project was supported by the National Natural Science Foundation of China (31372169, 61273289), Program of Ministry of Science and Technology of the Republic of China (2012FY111100), Special Fund for Entry-Exit Inspections and Quarantine (201210079, 201010042), K. C. Wong Education Foundation, Hong Kong, National Science Fund for Fostering Talents in Basic Research (special subjects in animal taxonomy, NSFC-J0630964/J0109), and special fund from General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (2014IK17). NR 37 TC 0 Z9 0 U1 2 U2 6 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD SEP 9 PY 2015 VL 4013 IS 3 BP 301 EP 346 PG 46 WC Zoology SC Zoology GA CQ8HT UT WOS:000360848600001 PM 26623901 ER PT J AU Piras, C Soggiu, A Greco, V Martino, PA Del Chierico, F Putignani, L Urbani, A Nally, JE Bonizzi, L Roncada, P AF Piras, Cristian Soggiu, Alessio Greco, Viviana Martino, Piera Anna Del Chierico, Federica Putignani, Lorenza Urbani, Andrea Nally, Jarlath E. Bonizzi, Luigi Roncada, Paola TI Mechanisms of antibiotic resistance to enrofloxacin in uropathogenic Escherichia coli in dog SO JOURNAL OF PROTEOMICS LA English DT Article DE Escherichia coli; Antibiotic resistance; Urinary tract infections; Enrofloxacin ID ENTERICA SEROVAR TYPHIMURIUM; URINARY-TRACT-INFECTIONS; OUTER-MEMBRANE VESICLES; FOOD-PRODUCING ANIMALS; URIDINE PHOSPHORYLASE; ANTIMICROBIAL RESISTANCE; SUPEROXIDE; PROTEIN; EXPRESSION; PROTEOMICS AB Escherichia coli (E. coli) urinary tract infections (UTIs) are becoming a serious problem both for pets and humans (zoonosis) due to the close contact and to the increasing resistance to antibiotics. This study has been performed in order to unravel the mechanism of induced enrofloxacin resistance in canine E. coli isolates that represent a good tool to study this pathology. The isolated E. coli has been induced with enrofloxacin and studied through 2D DICE and shotgun MS. Discovered differentially expressed proteins are principally involved in antibiotic resistance and linked to oxidative stress response, to DNA protection and to membrane permeability. Moreover, since enrofloxacin is an inhibitor of DNA gyrase, the overexpression of DNA starvation/stationary phase protection protein (Dsp) could be a central point to discover the mechanism of this clone to counteract the effects of enrofloxacin. In parallel, the dramatic decrease of the synthesis of the outer membrane protein W, which represents one of the main gates for enrofloxacin entrance, could explain additional mechanism of E. coli defense against this antibiotic. All 20 DICE and MS data have been deposited into the ProteomeXchange Consortium with identifier PXD002000 and DOI http://dx.doi.org/10.601/PXD002000. This article is part of a Special Issue entitled: HUPO 2014. (C) 2015 Elsevier B.V. All rights reserved. C1 [Piras, Cristian; Soggiu, Alessio; Martino, Piera Anna; Bonizzi, Luigi; Roncada, Paola] Univ Milan, Dipartimento Sci Vet & Sanita Pubbl, I-20133 Milan, Italy. [Greco, Viviana; Urbani, Andrea] Fdn Santa Lucia IRCCS, Rome, Italy. [Greco, Viviana; Urbani, Andrea] Univ Roma Tor Vergata, Dipartimento Med Sperimentale & Chirurg, I-00173 Rome, Italy. [Del Chierico, Federica; Putignani, Lorenza] IRCCS, Bambino Gesu Childrens Hosp, Metagen Unit, Rome, Italy. [Putignani, Lorenza] IRCCS, Bambino Gesu Childrens Hosp, Parasitol Unit, Rome, Italy. [Nally, Jarlath E.] ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Roncada, Paola] Ist Sperimentale Italiano L Spallanzani, Milan, Italy. RP Roncada, P (reprint author), Univ Milan, Dipartimento Sci Vet & Sanita Pubbl, Ist Sperimentale Italiano L Spallanzani, Via Celoria 10, I-20133 Milan, Italy. EM paola.roncada@istitutospallanzani.it RI Roncada, Paola/C-5263-2013; Del Chierico, Federica/J-4373-2016; putignani, lorenza/K-3409-2016; Piras, Cristian/K-6646-2016; Soggiu, Alessio/K-6724-2016 OI BONIZZI, LUIGI/0000-0002-9364-095X; Roncada, Paola/0000-0002-0114-5872; Del Chierico, Federica/0000-0002-4204-4736; putignani, lorenza/0000-0003-0134-2830; Piras, Cristian/0000-0002-6109-0834; Soggiu, Alessio/0000-0001-7680-6068 FU project "Piano di Sviluppo - UNIMI" FX Authors are grateful to the COST action 1002 FAP for the network provided and to the project "Piano di Sviluppo - UNIMI" (to A.S.) for the support. NR 76 TC 8 Z9 8 U1 4 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD SEP 8 PY 2015 VL 127 BP 365 EP 376 DI 10.1016/j.jprot.2015.05.040 PN B PG 12 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CV4RQ UT WOS:000364254600015 PM 26066767 ER PT J AU Utsunomiya, YT Ribeiro, ES Quintal, APN Sangalli, JR Gazola, VR Paula, HB Trinconi, CM Lima, VMF Perri, SHV Taylor, JF Schnabel, RD Sonstegard, TS Garcia, JF Nunes, CM AF Utsunomiya, Yuri T. Ribeiro, Erica S. Quintal, Amanda P. N. Sangalli, Juliano R. Gazola, Valquiria R. Paula, Henrique B. Trinconi, Cristiana M. Lima, Valeria M. F. Perri, Silvia H. V. Taylor, Jeremy F. Schnabel, Robert D. Sonstegard, Tad S. Garcia, Jose F. Nunes, Caris M. TI Genome-Wide Scan for Visceral Leishmaniasis in Mixed-Breed Dogs Identifies Candidate Genes Involved in T Helper Cells and Macrophage Signaling SO PLOS ONE LA English DT Article ID CANINE LEISHMANIASIS; POLYMORPHISM; INTERFERON; INFANTUM; IMMUNITY; BRAZIL; GAMMA; AREA AB We conducted a genome-wide scan for visceral leishmaniasis in mixed-breed dogs from a highly endemic area in Brazil using 149,648 single nucleotide polymorphism (SNP) markers genotyped in 20 cases and 28 controls. Using a mixed model approach, we found two candidate loci on canine autosomes 1 and 2. The positional association on chromosome 2 mapped to a predicted DNAse sensitive site in CD14+ monocytes that serve as a cis-regulatory element for the expression of interleukin alpha receptors 2 (IL2RA) and 15 (IL15RA). Both interleukins were previously found to lead to protective T helper 1 cell (Th1) response against Leishmania spp. in humans and mice. The associated marker on chromosome 1 was located between two predicted transcription factor binding sites regulating the expression of the transducin-like enhancer of split 1 gene (TLE1), an important player in Notch signaling. This pathway is critical for macrophage activity and CD4+ T cell differentiation into Th1 and T helper 2. Together, these findings suggest that the human and mouse model for protective response against Leishmania spp., which involves Th1 and macrophage modulation by interleukins 2, 15, gamma interferon and Notch signaling, may also hold for the canine model. C1 [Utsunomiya, Yuri T.; Garcia, Jose F.] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Sao Paulo, Brazil. [Ribeiro, Erica S.; Quintal, Amanda P. N.; Sangalli, Juliano R.; Gazola, Valquiria R.; Paula, Henrique B.; Trinconi, Cristiana M.; Lima, Valeria M. F.; Perri, Silvia H. V.; Garcia, Jose F.; Nunes, Caris M.] UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Saude & Prod Anim, Sao Paulo, Brazil. [Taylor, Jeremy F.; Schnabel, Robert D.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Sonstegard, Tad S.] USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD USA. RP Nunes, CM (reprint author), UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Saude & Prod Anim, Sao Paulo, Brazil. EM caris@fmva.unesp.br RI Trinconi, Cristiana/N-5208-2015; Utsunomiya, Yuri Tani/K-5214-2013 OI Trinconi, Cristiana/0000-0003-3493-7339; Utsunomiya, Yuri Tani/0000-0002-6526-8337 FU Sao Paulo Research Foundation (FAPESP) [2014/01095-8] FX YTU is supported by a scholarship from the Sao Paulo Research Foundation (FAPESP-http://www.fapesp.br/) (process 2014/01095-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 20 TC 6 Z9 6 U1 0 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 8 PY 2015 VL 10 IS 9 AR e0136749 DI 10.1371/journal.pone.0136749 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ8YQ UT WOS:000360897600011 PM 26348501 ER PT J AU Christensen, SA Huffaker, A Kaplan, F Sims, J Ziemann, S Doehlemann, G Ji, LX Schmitz, RJ Kolomiets, MV Alborn, HT Mori, N Jander, G Ni, XZ Sartor, RC Byers, S Abdo, Z Schmelz, EA AF Christensen, Shawn A. Huffaker, Alisa Kaplan, Fatma Sims, James Ziemann, Sebastian Doehlemann, Gunther Ji, Lexiang Schmitz, Robert J. Kolomiets, Michael V. Alborn, Hans T. Mori, Naoki Jander, Georg Ni, Xinzhi Sartor, Ryan C. Byers, Sara Abdo, Zaid Schmelz, Eric A. TI Maize death acids, 9-lipoxygenase-derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE oxylipin; 9-lipoxygenase; 10-oxo-11-phytoenoic acid; maize; defense ID HYPERSENSITIVE CELL-DEATH; JASMONIC ACID; 12-OXO-PHYTODIENOIC ACID; ARABIDOPSIS-THALIANA; PLANT DEFENSE; LIPID-PEROXIDATION; CYSTEINE PROTEASES; TOBACCO-LEAVES; OXYLIPINS; RESPONSES AB Plant damage promotes the interaction of lipoxygenases (LOXs) with fatty acids yielding 9-hydroperoxides, 13-hydroperoxides, and complex arrays of oxylipins. The action of 13-LOX on linolenic acid enables production of 12-oxo-phytodienoic acid (12-OPDA) and its downstream products, termed "jasmonates." As signals, jasmonates have related yet distinct roles in the regulation of plant resistance against insect and pathogen attack. A similar pathway involving 9-LOX activity on linolenic and linoleic acid leads to the 12-OPDA positional isomer, 10-oxo-11-phytodienoic acid (10-OPDA) and 10-oxo-11-phytoenoic acid (10-OPEA), respectively; however, physiological roles for 9-LOX cyclopentenones have remained unclear. In developing maize (Zea mays) leaves, southern leaf blight (Cochliobolus heterostrophus) infection results in dying necrotic tissue and the localized accumulation of 10-OPEA, 10-OPDA, and a series of related 14- and 12-carbon metabolites, collectively termed "death acids." 10-OPEA accumulation becomes wound inducible within fungal-infected tissues and at physiologically relevant concentrations acts as a phytoalexin by suppressing the growth of fungi and herbivores including Aspergillus flavus, Fusarium verticillioides, and Helicoverpa zea. Unlike previously established maize phytoalexins, 10-OPEA and 10-OPDA display significant phytotoxicity. Both 12-OPDA and 10-OPEA promote the transcription of defense genes encoding glutathione S transferases, cytochrome P450s, and pathogenesis-related proteins. In contrast, 10-OPEA only weakly promotes the accumulation of multiple protease inhibitor transcripts. Consistent with a role in dying tissue, 10-OPEA application promotes cysteine protease activation and cell death, which is inhibited by overexpression of the cysteine protease inhibitor maize cystatin-9. Unlike jasmonates, functions for 10-OPEA and associated death acids are consistent with specialized roles in local defense reactions. C1 [Christensen, Shawn A.; Alborn, Hans T.] USDA ARS, Chem Res Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Huffaker, Alisa; Sartor, Ryan C.; Schmelz, Eric A.] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA. [Kaplan, Fatma] Kaplan Schiller Res, Gainesville, FL 32604 USA. [Sims, James] ETH, Dept Environm Syst Sci, CH-8092 Zurich, Switzerland. [Ziemann, Sebastian; Doehlemann, Gunther] Univ Cologne, Cologne Bioctr, Inst Bot, D-50674 Cologne, Germany. [Ji, Lexiang] Univ Georgia, Inst Bioinformat, Athens, GA 30602 USA. [Schmitz, Robert J.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. [Kolomiets, Michael V.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Mori, Naoki] Kyoto Univ, Grad Sch Agr, Kyoto 6068502, Japan. [Jander, Georg] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Ni, Xinzhi] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Byers, Sara; Abdo, Zaid] USDA ARS, South Atlantic Area Off, Athens, GA 30605 USA. RP Schmelz, EA (reprint author), Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA. EM eschmelz@ucsd.edu RI Abdo, Zaid/E-5176-2017; OI Abdo, Zaid/0000-0002-8272-7734; Doehlemann, Gunther/0000-0002-7353-8456 FU National Science Foundation (NSF) Division of Materials Research Award [1157490]; State of Florida; NIH [S10RR031637]; US Department of Agriculture (USDA)-Agricultural Research Service Projects [6615-21000-010-00/-22000-027-00]; NSF Division of Integrative Organismal Systems Competitive Award [1139329]; University of California, San Diego startup funds FX Special thanks to Wayne Davis for the kind donation of maize tissues and James R. Rocca for facilitating NMR experiments at the McKnight Brain Institute (National High Magnetic Field Laboratory, Advanced Magnetic Resonance Imaging and Spectroscopy Facility). NMR work was supported by the National Science Foundation (NSF) Division of Materials Research Award 1157490, the State of Florida, and NIH Award S10RR031637. Research was funded by US Department of Agriculture (USDA)-Agricultural Research Service Projects 6615-21000-010-00/-22000-027-00, NSF Division of Integrative Organismal Systems Competitive Award 1139329, and University of California, San Diego startup funds allocated to E.A.S. NR 46 TC 9 Z9 10 U1 6 U2 37 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP 8 PY 2015 VL 112 IS 36 BP 11407 EP 11412 DI 10.1073/pnas.1511131112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR0FW UT WOS:000360994900069 PM 26305953 ER PT J AU Parnell, S Gottwald, TR Cunniffe, NJ Chavez, VA van den Bosch, F AF Parnell, S. Gottwald, T. R. Cunniffe, N. J. Chavez, V. Alonso van den Bosch, F. TI Early detection surveillance for an emerging plant pathogen: a rule of thumb to predict prevalence at first discovery SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE surveillance; epidemic model; theoretical biology; citrus disease; Huanglongbing; invasive species ID CITRUS CANKER; MOUTH-DISEASE; EPIDEMIC; BIOLOGY; DIEBACK; FLORIDA AB Emerging plant pathogens are a significant problem for conservation and food security. Surveillance is often instigated in an attempt to detect an invading epidemic before it gets out of control. Yet in practice many epidemics are not discovered until already at a high prevalence, partly due to a lack of quantitative understanding of how surveillance effort and the dynamics of an invading epidemic relate. We test a simple rule of thumb to determine, for a surveillance programme taking a fixed number of samples at regular intervals, the distribution of the prevalence an epidemic will have reached on first discovery (discovery-prevalence) and its expectation E(q(star)). We show that E(q(star)) = r / (N / Delta), i.e. simply the rate of epidemic growth divided by the rate of sampling; where r is the epidemic growth rate, N is the sample size and Delta is the time between sampling rounds. We demonstrate the robustness of this rule of thumb using spatio-temporal epidemic models as well as data from real epidemics. Our work supports the view that, for the purposes of early detection surveillance, simple models can provide useful insights in apparently complex systems. The insight can inform decisions on surveillance resource allocation in plant health and has potential applicability to invasive species generally. C1 [Parnell, S.; Chavez, V. Alonso; van den Bosch, F.] Rothamsted Res, Computat & Syst Biol, Harpenden AL5 2JQ, Herts, England. [Parnell, S.] Univ Salford, Sch Environm & Life Sci, Salford M5 4WT, Lancs, England. [Gottwald, T. R.] USDA ARS, Ft Pierce, FL 34945 USA. [Cunniffe, N. J.] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England. RP Parnell, S (reprint author), Rothamsted Res, Computat & Syst Biol, Harpenden AL5 2JQ, Herts, England. EM s.r.parnell@salford.ac.uk OI Cunniffe, Nik/0000-0002-3533-8672 FU US Department of Agriculture Farm Bill; U.K. Biotechnology and Biological Sciences Research Council (BBSRC); Bill and Melinda Gates Foundation FX S.F., T.R.G. and F.V.B. received funding from the US Department of Agriculture Farm Bill. S.F., F.V.B. and V.A.C. received support from the U.K. Biotechnology and Biological Sciences Research Council (BBSRC) through Rothamsted Research. F.V.B. received funding from the Bill and Melinda Gates Foundation. NR 27 TC 2 Z9 2 U1 4 U2 13 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD SEP 7 PY 2015 VL 282 IS 1814 BP 47 EP 55 AR 20151478 DI 10.1098/rspb.2015.1478 PG 9 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA CU2OC UT WOS:000363361800006 ER PT J AU MacDicken, K Reams, G de Freitas, J AF MacDicken, Kenneth Reams, Greg de Freitas, Joberto TI Introduction to the Changes in Global Forest Resources from 1990 to 2015 SO FOREST ECOLOGY AND MANAGEMENT LA English DT Editorial Material C1 [MacDicken, Kenneth] UN, Food & Agr Org, Rome, Italy. [Reams, Greg] US Forest Serv, USDA, Natl Off, Arlington, VA 22209 USA. [de Freitas, Joberto] SCEN, Minist Environm, Brazilian Forest Serv, BR-70818900 Brasilia, DF, Brazil. RP MacDicken, K (reprint author), UN, Food & Agr Org, Rome, Italy. EM kenneth.macdicken@fao.org NR 0 TC 1 Z9 1 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD SEP 7 PY 2015 VL 352 BP 1 EP 2 DI 10.1016/j.foreco.2015.06.018 PG 2 WC Forestry SC Forestry GA CS0UH UT WOS:000361777200001 ER PT J AU Keenan, RJ Reams, GA Achard, F de Freitas, JV Grainger, A Lindquist, E AF Keenan, Rodney J. Reams, Gregory A. Achard, Frederic de Freitas, Joberto V. Grainger, Alan Lindquist, Erik TI Dynamics of global forest area: Results from the FAO Global Forest Resources Assessment 2015 SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Forest; Deforestation; Forest transition; Land use; Land cover; Remote sensing ID LAND-COVER CHANGE; TROPICAL DEFORESTATION; TRANSITIONS; REGIONS; RATES AB The area of land covered by forest and trees is an important indicator of environmental condition. This study presents and analyses results from the Global Forest Resources Assessment 2015 (FRA 2015) of the Food and Agriculture Organization of the United Nations. FRA 2015 was based on responses to surveys by individual countries using a common reporting framework, agreed definitions and reporting standards. Results indicated that total forest area declined by 3%, from 4128 M ha in 1990 to 3999 M ha in 2015. The annual rate of net forest loss halved from 73 M ha y(-1) in the 1990s to 3.3 M ha y(-1) between 2010 and 2015. Natural forest area declined from 3961 M ha to 3721 M ha between 1990 and 2015, while planted forest (including rubber plantations) increased from 168 M ha to 278 M ha. From 2010 to 2015, tropical forest area declined at a rate of 5.5 M ha y(-1) - only 58% of the rate in the 1990s - while temperate forest area expanded at a rate of 2.2 M ha y(-1). Boreal and sub-tropical forest areas showed little net change. Forest area expanded in Europe, North America, the Caribbean, East Asia, and Western-Central Asia, but declined in Central America, South America, South and Southeast Asia and all three regions in Africa. Analysis indicates that, between 1990 and 2015, 13 tropical countries may have either passed through their forest transitions from net forest loss to net forest expansion, or continued along the path of forest expansion that follows these transitions. Comparing FRA 2015 statistics with the findings of global and pan-tropical remote-sensing forest area surveys was challenging, due to differences in assessment periods, the definitions of forest and remote sensing methods. More investment in national and global forest monitoring is needed to provide better support for international initiatives to increase sustainable forest management and reduce forest loss, particularly in tropical countries. (C) 2015 Published by Elsevier B.V. C1 [Keenan, Rodney J.] Univ Melbourne, Sch Ecosyst & Forest Sci, Carlton, Vic 3053, Australia. [Reams, Gregory A.] US Forest Serv, USDA, Natl Off, Arlington, VA 22209 USA. [Achard, Frederic] Commiss European Communities, Joint Res Ctr, I-21027 Ispra, VA, Italy. [de Freitas, Joberto V.] SCEN, Minist Environm, Brazilian Forest Serv, BR-70818900 Brasilia, DF, Brazil. [Grainger, Alan] Univ Leeds, Sch Geog, Leeds LS2 9JT, W Yorkshire, England. [Lindquist, Erik] UN Food & Agr Org, I-00153 Rome, Italy. RP Keenan, RJ (reprint author), Univ Melbourne, Sch Ecosyst & Forest Sci, 221 Bouverie St, Carlton, Vic 3053, Australia. EM rkeenan@unimelb.edu.au; greams@fs.fed.us; frederic.achard@jrc.ec.europa.eu; joberto.freitas@florestal.gov.br; a.grainger@leeds.ac.uk; Erik.Lindquist@fao.org OI Keenan, Rodney/0000-0002-2804-4975 NR 60 TC 39 Z9 40 U1 33 U2 118 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD SEP 7 PY 2015 VL 352 BP 9 EP 20 DI 10.1016/j.foreco.2015.06.014 PG 12 WC Forestry SC Forestry GA CS0UH UT WOS:000361777200003 ER PT J AU Miura, S Amacher, M Hofer, T San-Miguel-Ayanz, J Ernawati Thackway, R AF Miura, Satoru Amacher, Michael Hofer, Thomas San-Miguel-Ayanz, Jesus Ernawati Thackway, Richard TI Protective functions and ecosystem services of global forests in the past quarter-century SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Sustainable forest management; Multiple functions; Forest conservation; Ecosystem value; Criteria; Indicator ID SLOPE STABILITY; SOIL-EROSION; MANAGEMENT; CHINA; CERTIFICATION; VEGETATION; PATTERN AB The world's forests provide fundamental protection of soil and water resources as well as multiple ecosystem services and cultural or spiritual values. We summarized the FRA 2015 data for protective functions and ecosystem services, and analyzed increasing or decreasing trends of protective areas. The global forest area managed for protection of soil and water was 1.002 billion ha as of 2015, which was 25.1% of all global forested areas. Protective forests have increased by 0.181 billion ha over the past 25 years mainly because more countries are now reporting protective forest areas (139 in 2015 vs 114 in 1990). However, average percentage of designated for protective forests did not change significantly from 1990 to 2015. Global forest area managed for ecosystem services is also now at 25.4% of global total forest area and has changed little over the past 25 years. Among the twelve categories of protective forests, flood control, public recreation, and cultural services increased both in terms of percentage of total forest area and the number of reporting countries. Public awareness of the importance of forest resources for functions and services other than production continues to increase as evidenced by the increase of protective forest designations and reporting in many countries. Percentages of total forest area designated for both protective forests and ecosystem services show a dual-peak distribution of numbers of countries concentrated at 0% and 100%. This suggests a socio-economic influence for the designations. We examined five case study countries (Australia, Canada, China, Kenya, and Russia). The most dramatic changes in the past 25 years have been in China where protective forests for soil and water resources increased from about 12% to 28% of forest area. The Russian Federation has also increased percentages of forest area devoted to soil and water resource protection and delivery of ecosystem services. Australia is now reporting in more protective forest categories whereas Kenya and Canada changed little. These five countries have their own classification of forest functions and recalculation methods of reporting for FRA 2015 were different. This demonstrates the difficulty in establishing a universal common designation scheme for multi-functions of forest. Production of more accurate assessments by further improvements in the reporting framework and data quality would help advance the value of FRA as the unique global database for forest functions integrated between forest ecosystems and social sciences. (C) 2015 Published by Elsevier B.V. C1 [Miura, Satoru] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan. [Amacher, Michael] US Forest Serv, Logan Forestry Sci Lab, Rocky Mt Res Stn, Logan, UT 84321 USA. [Hofer, Thomas] UN, Food & Agr Org, Dept Forestry, Watershed Management & Mt, I-00153 Rome, Italy. [San-Miguel-Ayanz, Jesus] Commiss European Communities, DG Joint Res Ctr, Unit Forest Resources & Climate, I-21027 Ispra, Italy. [Ernawati] Minist Forestry, Data & Informat Forest Management Unit, Jakarta, Indonesia. [Ernawati] Minist Forestry, Land Preparat Forestry, Jakarta, Indonesia. [Thackway, Richard] Univ Queensland, Sch Geog, Planning & Environm Management, Brisbane St Lucia, Qld 4072, Australia. RP Miura, S (reprint author), Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan. EM a-miura@mail.ecc.u-tokyo.ac.jp RI Miura, Satoru/B-1388-2013; xiao, qiang/S-7367-2016 OI Miura, Satoru/0000-0001-6439-1977; NR 61 TC 8 Z9 8 U1 4 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD SEP 7 PY 2015 VL 352 BP 35 EP 46 DI 10.1016/j.foreco.2015.03.039 PG 12 WC Forestry SC Forestry GA CS0UH UT WOS:000361777200005 ER PT J AU Morales-Hidalgo, D Oswalt, SN Somanathan, E AF Morales-Hidalgo, David Oswalt, Sonja N. Somanathan, E. TI Status and trends in global primary forest, protected areas, and areas designated for conservation of biodiversity from the Global Forest Resources Assessment 2015 SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE FRA 2015; Biodiversity conservation; Global Forest Resource Assessment; Primary forest; Protected areas ID LAND-USE; DEFORESTATION; TRADE AB The global community has recognized the importance of forests for biodiversity, and has prioritized the preservation of forest biodiversity and ecosystem functions through multiple multilateral agreements and processes such as the Convention on Biodiversity's Aichi Targets and the Millennium Development Goals. The Global Forest Resources Assessment (FRA) provides one mechanism for tracking progress toward such goals in three particular areas: primary forest area, protected forest areas, and areas designated for the conservation of biodiversity. In this paper, we quantify current area and trends in forest areas designated for the conservation of biodiversity, protected forest areas, and primary forests by country and biome; and examine the association between total forest area and measures of protection, per-capita income, and population. The overall findings suggest that countries are increasingly protecting forests of ecological significance at the global scale (7.7% of forests were protected in 1990 rising to 16.3% in 2015), with a strong upward trend in protected areas in the tropical domain (from 12% in 1990 to 26.3% in 2015). However, primary forest area has declined by 2.5% globally and by 10% in the tropics over the period 1990-2015 (using data for countries that reported in all years). Given that many species in the tropics are endemic to primary forests, losses in that climatic domain continue to be of concern, although the rate of decline appears to be slowing. Using multiple regression analysis, we find that a 1% increase in protected area or area designated for biodiversity conservation within a country is associated with an increase in total forest area in that country of about 0.03% (p < 0.05). A 1% within-country increase in population density and per capita GDP are associated with a decrease in forest area of about 0.2% (p < 0.01) and an increase in forest area of about 0.08% (p < 0.05) respectively. Our findings also indicate that, since FRA is used as one mechanism for tracking progress toward goals like the AICHI Biodiversity Targets, country correspondents may require additional assistance toward reporting on primary forest, protected forest, and biodiversity conservation statistics. (C) 2015 Published by Elsevier B.V. C1 [Morales-Hidalgo, David] UN, Food & Agr Org, Dept Forestry, I-00153 Rome, Italy. [Oswalt, Sonja N.] US Forest Serv, USDA, Southern Res Stn, Forest Inventory & Anal, Knoxville, TN 37922 USA. [Somanathan, E.] Indian Stat Inst, Econ & Planning Unit, New Delhi 110016, India. RP Somanathan, E (reprint author), Indian Stat Inst, Econ & Planning Unit, 7 Shaheed Jeet Singh Marg, New Delhi 110016, India. EM David.Morales@fao.org NR 34 TC 5 Z9 5 U1 11 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD SEP 7 PY 2015 VL 352 BP 68 EP 77 DI 10.1016/j.foreco.2015.06.011 PG 10 WC Forestry SC Forestry GA CS0UH UT WOS:000361777200008 ER PT J AU Zhang, ML Temirbayeva, K Sanderson, SC Chen, X AF Zhang, Ming-Li Temirbayeva, Kamshat Sanderson, Stewart C. Chen, Xi TI Young dispersal of xerophil Nitraria lineages in intercontinental disjunctions of the Old World SO SCIENTIFIC REPORTS LA English DT Article ID TIBETAN PLATEAU UPLIFT; HISTORICAL BIOGEOGRAPHY; CENTRAL-ASIA; PHYLOGENY; MIOCENE; EVOLUTION; CLIMATE; CHINA; BASIN; DIVERSIFICATION AB Many cases of intercontinental disjunct distributions of seed plants have been investigated, however few have concerned the continents of Eurasia (mainly Central Asia), Africa, and Australia, especially the xerophytic lineages are lacking. Nitraria (Nitrariaceae) is just one of these xerophytic lineages. Previous Nitraria studies have hypothesized either Africa as the ancient center, with dispersals to Australia and Eurasia, or alternatively Central Asia, due to a concentration of endemism and diversity there. Our findings show eastern Central Asia, i.e. the eastern Tethys, to be the correct place of origin. Dispersal westward to Africa occurred during the late Oligocene to Pliocene, whereas dispersal to Australia from western Central Asia was young since Pliocene 2.61 Ma. Two related tetraploids are indicated to have diversified in eastern Central Asia at approximately 5.89 Ma, while the Australian tetraploid N. billardieri, is an independently derived, recent dispersal from western Central Asia. C1 [Zhang, Ming-Li; Temirbayeva, Kamshat; Chen, Xi] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. [Zhang, Ming-Li] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Sanderson, Stewart C.] US Forest Serv, Shrub Sci Lab, Intermt Res Stn, USDA, Ogden, UT 84601 USA. RP Zhang, ML (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. EM zhangml@ibcas.ac.cn; chenxi@ms.xjb.ac.cn FU China National Key Basic Research Program [2014CB954201]; Innovation Research Group Program of Chinese Academy of Sciences and State Administration of Foreign Experts Affairs of China [KZCX2-YW-T09]; Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences FX We are grateful to Xiao-Guo Xiang (Beijing, China) and Hong-Xiang Zhang (Urumqi, China) for their kind help in data processing, to Vyacheslav V. Byalt (St. Petersburg, Russia), Alexander P. Sukhorukov (Moscow, Russia), and David M. Williams (London, UK) for their valuable discussions. This study was funded by China National Key Basic Research Program (2014CB954201), Innovation Research Group Program of Chinese Academy of Sciences and State Administration of Foreign Experts Affairs of China (KZCX2-YW-T09), and Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences. NR 60 TC 0 Z9 0 U1 2 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD SEP 7 PY 2015 VL 5 AR 13840 DI 10.1038/srep13840 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ6CQ UT WOS:000360692400001 PM 26343223 ER PT J AU Willett, JL Finkenstadt, VL AF Willett, Julious L. Finkenstadt, Victoria L. TI Starch-poly(acrylamide-co-2-acrylamido-2-methylpropanesulfonic acid) graft copolymers prepared by reactive extrusion SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE grafting; polysaccharides; properties and characterization; swelling; thermoplastics ID WATER-SOLUBLE COPOLYMERS; SULFONATED CO-MONOMERS; STARCH; ACRYLAMIDE; POLYACRYLAMIDE; POLYMERIZATION; HYDROGELS; GEL AB Graft copolymers of starch with acrylamide and 2-acrylamido-2-methylpropanesulfonic acid (AMPS) were prepared by reactive extrusion in a twin-screw extruder. The weight ratio of total monomer to starch was fixed at 1 : 3, while the molar fraction of AMPS in the monomer feed ranged from 0 to 0.119. Monomer to polymer conversions were 85% or greater, with grafting efficiencies of 68% (highest AMPS content) to 85% (no AMPS). Absorbency in distilled water at pH 7 increased linearly with the mole fraction AMPS in the grafted polymer, while absorbencies in 0.9% NaCl were independent of AMPS content. When swollen in water/ethanol mixtures, swelling decreased gradually with increasing ethanol volume fraction, followed by a large decrease over a narrow ethanol concentration. This behavior is similar to that observed for AMPS-acrylamide gels. The swelling properties suggest these graft copolymers may have applications as responsive materials. (C) 2015 Wiley Periodicals, Inc. C1 [Willett, Julious L.; Finkenstadt, Victoria L.] ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Finkenstadt, VL (reprint author), ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM victoria.finkenstadt@ars.usda.gov FU USDA [306, 157] FX The authors gratefully acknowledge the technical assistance of B. Jasberg, G. Grose, R. Haig, and K. Hornback. This work was authorized under USDA National Program 306: Quality and Utilization of Agricultural Products and Project 157: Advanced Starch-based Materials (National Program Leader: Dr. Robert Fireovid). NR 22 TC 0 Z9 0 U1 2 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD SEP 5 PY 2015 VL 132 IS 33 AR 42405 DI 10.1002/app.42405 PG 7 WC Polymer Science SC Polymer Science GA CK1HP UT WOS:000355958300019 ER PT J AU Hwang, IH Swenson, DC Gloer, JB Wicklow, DT AF Hwang, In Hyun Swenson, Dale C. Gloer, James B. Wicklow, Donald T. TI Pestaloporonins: Caryophyllene-Derived Sesquiterpenoids from a Fungicolous Isolate of Pestalotiopsis sp. SO ORGANIC LETTERS LA English DT Article ID FUNGUS PORONIA-PUNCTATA; TAXUS-BREVIFOLIA; METABOLITES; PUNCTAPORONINS; REARRANGEMENT; ASPERGILLUS; MARINE AB Three new sesquiterpenoids (pestaloporonins A C ; 1-3) related to the caryophyllene-derived punctaporonins were isolated from cultures of a fungicolous isolate of Pestalotiopsis sp. The structures of 1-3 were determined by analysis of NMR and HRMS data, and the structure of 1, including its absolute configuration, was confirmed by X-ray crystallographic analysis. Compounds 1 and 2 contain new bicyclic and tricyclic ring systems, respectively. C1 [Hwang, In Hyun; Swenson, Dale C.; Gloer, James B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, Donald T.] USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. RP Gloer, JB (reprint author), Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. EM james-gloer@uiowa.edu FU National Science Foundation [CHE-1011847]; U.S. Department of Energy, Office of Energy Sciences Materials Sciences Division [DE-AC02-05CH11231] FX Financial support was provided by a grant from the National Science Foundation (CHE-1011847). Crystallographic data were collected through the SCrALS (Service Crystallography at Advanced Light Source) program at the Small-Crystal Crystallography Beam line 11.3.1 at the Advanced Light Source (ALS), Lawrence Berkeley National Laboratory. The ALS is supported by the U.S. Department of Energy, Office of Energy Sciences Materials Sciences Division, under Contract DE-AC02-05CH11231. NR 26 TC 4 Z9 4 U1 5 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1523-7060 EI 1523-7052 J9 ORG LETT JI Org. Lett. PD SEP 4 PY 2015 VL 17 IS 17 BP 4284 EP 4287 DI 10.1021/acs.orglett.5b02080 PG 4 WC Chemistry, Organic SC Chemistry GA CR1LV UT WOS:000361087200041 PM 26287562 ER PT J AU Liu, M Bischoff, KM Gill, JJ Mire-Criscione, MD Berry, JD Young, R Summer, EJ AF Liu, Mei Bischoff, Kenneth M. Gill, Jason J. Mire-Criscione, Miranda D. Berry, Joel D. Young, Ry Summer, Elizabeth J. TI Bacteriophage application restores ethanol fermentation characteristics disrupted by Lactobacillus fermentum SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Lactic acid bacteria; Lactobacillus fermentum; Ethanol fermentation; Bacteriophage ID LACTIC-ACID BACTERIA; SACCHAROMYCES-CEREVISIAE; LACTOCOCCAL PHAGES; ACETIC-ACID; GENOME; GROWTH; P335; IDENTIFICATION; CONTAMINATION; DIVERSITY AB Background: Contamination of corn mash by lactic acid bacteria (LAB) reduces the efficiency of the ethanol fermentation process. The industry relies heavily on antibiotics for contamination control and there is a need to develop alternative methods. The goals of this study were to determine the diversity and abundance of bacteria contaminating commercial ethanol fermentations, and to evaluate the potential of anti-LAB bacteriophages in controlling production losses. Results: Bacterial populations in 27 corn mash samples collected from nine different commercial plants were determined by pyrosequencing of 16S rRNA amplicons. The results showed that the most abundant bacteria (> 50 % of total population) in 24 of the 27 samples included LAB genera such as Lactobacillus, Streptococcus, Lactococcus, Weissella, Enterococcus, and Pediococcus. Lactobacillus was identified as the most prevalent genus at all fermentation stages in all plants, accounting for between 2.3 and 93.7 % of each population and constituting the major genus (> 50 %) in nine samples from five plants and the most abundant genus in five other samples. Lactobacillus species, including L. delbrueckii, L. fermentum, L. mucosae, and L. reuteri were the most well-represented species. Two bacteriophages that target L. fermentum strains from ethanol plants, vB_LfeS_EcoSau and vB_LfeM_EcoInf (EcoSau and EcoInf), were isolated and characterized as a siphophage and a myophage, respectively. Analysis of the 31,703 bp genome of EcoSau revealed its similarity to the P335-like phage group, and the 106,701 bp genome of phage EcoInf was determined to be a novel phage type despite its distant relationship to the SPO1-like phages. Addition of phages EcoSau and EcoInf to L. fermentum-contaminated corn mash fermentation models restored the yields of ethanol and reduced levels of residual glucose, lactic acid, and acetic acid to that comparable to the infection-free control. Conclusions: This study provides detailed insight into the microbiota contaminating commercial ethanol fermentations, and highlights the abundance of LAB, especially L. delbrueckii, L. fermentum, L. mucosae, and L. reuteri, in the process. This study suggests that phages with broad coverage of major LAB species can be applied directly to corn mash for antibiotic-free control of contamination in the ethanol fermentation industry. C1 [Liu, Mei; Mire-Criscione, Miranda D.; Summer, Elizabeth J.] Ecolyse Inc, College Stn, TX 77845 USA. [Bischoff, Kenneth M.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Gill, Jason J.; Berry, Joel D.; Young, Ry] Texas A&M Univ, Ctr Phage Technol, College Stn, TX 77843 USA. [Gill, Jason J.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Young, Ry] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA. RP Summer, EJ (reprint author), Ecolyse Inc, 11142 Hopes Creek Rd, College Stn, TX 77845 USA. EM liz@ecolyse.com OI Young, Ry/0000-0001-8001-2914; Gill, Jason/0000-0002-9494-6053 FU NSF SBIR [1230441]; Texas A&M and Texas A&M AgriLife Research FX This work was funded by NSF SBIR Grant No. 1230441 and Texas A&M and Texas A&M AgriLife Research. We thank the staff at various commercial ethanol plants, especially Dan Matlick at Lincolnway Energy (Nevada, IA) and Sivaraj Chokkaram at Green Plains Holdings II LLC (Riga, MI), for providing corn mash fermentation samples and valuable discussion on contamination controls. NR 45 TC 0 Z9 0 U1 3 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD SEP 4 PY 2015 VL 8 AR 132 DI 10.1186/s13068-015-0325-9 PG 13 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA CQ5WQ UT WOS:000360676800001 PM 26339290 ER PT J AU Liu, JJ Sturrock, RN Sniezko, RA Williams, H Benton, R Zamany, A AF Liu, Jun-Jun Sturrock, Rona N. Sniezko, Richard A. Williams, Holly Benton, Ross Zamany, Arezoo TI Transcriptome analysis of the white pine blister rust pathogen Cronartium ribicola: de novo assembly, expression profiling, and identification of candidate effectors SO BMC GENOMICS LA English DT Article DE Cronartium ribicola; Effector; Pathogenicity; RNA-seq; Transcriptome profiling ID SECRETED PROTEINS; GENE-EXPRESSION; HOST-RESISTANCE; USTILAGO-MAYDIS; FLAX RUST; GENOME; FUNGI; WHEAT; ANNOTATION; SEQUENCE AB Background: The fungus Cronartium ribicola (Cri) is an economically and ecologically important forest pathogen that causes white pine blister rust (WPBR) disease on five-needle pines. To cause stem cankers and kill white pine trees the fungus elaborates a life cycle with five stages of spore development on five-needle pines and the alternate host Ribes plants. To increase our understanding of molecular WP-BR interactions, here we report genome-wide transcriptional profile analysis of C. ribicola using RNA-seq. Results: cDNA libraries were constructed from aeciospore, urediniospore, and western white pine (Pinus monticola) tissues post Cri infection. Over 200 million RNA-seq 100-bp paired-end (PE) reads from rust fungal spores were de novo assembled and a reference transcriptome was generated with 17,880 transcripts that were expressed from 13,629 unigenes. A total of 734 unique proteins were predicted as a part of the Cri secretome from complete open reading frames (ORFs), and 41 % of them were Cronartium-specific. This study further identified a repertoire of candidate effectors and other pathogenicity determinants. Differentially expressed genes (DEGs) were identified to gain an understanding of molecular events important during the WPBR fungus life cycle by comparing Cri transcriptomes at different infection stages. Large-scale changes of in planta gene expression profiles were observed, revealing that multiple fungal biosynthetic pathways were enhanced during mycelium growth inside infected pine stem tissues. Conversely, many fungal genes that were up-regulated at the urediniospore stage appeared to be signalling components and transporters. The secreted fungal protein genes that were up-regulated in pine needle tissues during early infection were primarily associated with cell wall modifications, possibly to mask the rust pathogen from plant defenses. Conclusion: This comprehensive transcriptome profiling substantially improves our current understanding of molecular WP-BR interactions. The repertoire of candidate effectors and other putative pathogenicity determinants identified here are valuable for future functional analysis of Cri virulence and pathogenicity. C1 [Liu, Jun-Jun; Sturrock, Rona N.; Williams, Holly; Benton, Ross; Zamany, Arezoo] Nat Resources Canada, Pacific Forestry Ctr, Canadian Forest Serv, Victoria, BC V8Z 1M5, Canada. [Sniezko, Richard A.] USDA Forest Serv, Dorena Genet Resource Ctr, Cottage Grove, OR 97424 USA. RP Liu, JJ (reprint author), Nat Resources Canada, Pacific Forestry Ctr, Canadian Forest Serv, 506 West Burnside Rd, Victoria, BC V8Z 1M5, Canada. EM Jun-Jun.Liu@NRCan-RNCan.gc.ca FU PFC-CFS DG's fund; CFS-A base fund; CFS-GRDI fund FX We are grateful to Aimin Guan, Gary Zhang, and Hao Chen for computer programming and bioinformatic analyses; Gary Roke, Rich Hunt, Angelia Kegley and colleagues at DGRC for sample collection; Terry Holmes and Gary Roke for microscopy work. This research was supported partly by PFC-CFS DG's fund, CFS-A base fund, and the CFS-GRDI fund awarded to J.-J.L. NR 73 TC 3 Z9 3 U1 12 U2 48 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD SEP 4 PY 2015 VL 16 AR 678 DI 10.1186/s12864-015-1861-1 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CQ4XF UT WOS:000360607200002 PM 26338692 ER PT J AU Liefert, WM Liefert, O AF Liefert, William M. Liefert, Olga TI Russia's potential to increase grain production by expanding area SO EURASIAN GEOGRAPHY AND ECONOMICS LA English DT Article DE Russia; Russian agriculture; transition; grain; grain trade; area expansion ID FOOD SECURITY; AGRICULTURE AB Since 2000, Russia has substantially increased grain production and exports. The grain output growth has come from a rise in yields rather than area. After falling heavily during the 1990s, grain area stabilized during the 2000s and has remained flat, at about two-thirds the level of the late Soviet period. Using data on the regional structure of Russian grain production costs, this article examines the country's potential to increase grain output further by returning the lost grain area to production. The analysis finds that if grain area were to grow beyond a certain level, one that is still well below the level of the late Soviet period, production costs would rise steeply. Therefore, any major expansion in grain area would require that world grain prices rise considerably beyond their level in the early 2010s to cover the high marginal costs of production. C1 [Liefert, William M.; Liefert, Olga] USDA, Econ Res Serv, 355 E St SW, Washington, DC 20024 USA. RP Liefert, WM (reprint author), USDA, Econ Res Serv, 355 E St SW, Washington, DC 20024 USA. EM wliefert@ers.usda.gov NR 39 TC 0 Z9 0 U1 5 U2 6 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 1538-7216 EI 1938-2863 J9 EURASIAN GEOGR ECON JI Eurasian Geogr. Econ. PD SEP 3 PY 2015 VL 56 IS 5 BP 505 EP 523 DI 10.1080/15387216.2015.1101353 PG 19 WC Area Studies; Geography SC Area Studies; Geography GA DG1IT UT WOS:000371820800003 ER PT J AU Nichols, TA Fischer, JW Spraker, TR Kong, QZ VerCauteren, KC AF Nichols, Tracy A. Fischer, Justin W. Spraker, Terry R. Kong, Qingzhong VerCauteren, Kurt C. TI CWD prions remain infectious after passage through the digestive system of coyotes (Canis latrans) SO PRION LA English DT Article DE chronic wasting disease; coyotes; environmental contamination; feces; prions; scavengers; transmission ID CHRONIC WASTING DISEASE; CERVUS-ELAPHUS-NELSONI; MULE DEER; SPONGIFORM ENCEPHALOPATHY; ODOCOILEUS-HEMIONUS; TRANSMISSION; PROTEIN; ELK; STRATEGIES; CARCASSES AB Chronic wasting disease (CWD) is a geographically expanding prion disease of wild and captive cervids in North America. Disease can be transmitted directly, animal to animal, or indirectly via the environment. CWD contamination can occur residually in the environment via soil, water, and forage following deposition of bodily fluids such as urine, saliva, and feces, or by the decomposition of carcasses. Recent work has indicated that plants may even take up prions into the stems and leaves. When a carcass or gut pile is present in the environment, a large number of avian and mammalian species visit and consume the carrion. Additionally, predators like coyotes, likely select for disease-compromised cervids. Natural cross-species CWD transmission has not been documented, however, passage of infectious prion material has been observed in the feces of crows. In this study we evaluated the ability of CWD-infected brain material to pass through the gastrointestinal tract of coyotes (Canis latrans) following oral ingestion, and be infectious in a cervidized transgenic mouse model. Results from this study indicate that coyotes can pass infectious prions via their feces for at least 3 days post ingestion, demonstrating that mammalian scavengers could contribute to the translocation and contamination of CWD in the environment. C1 [Nichols, Tracy A.; Fischer, Justin W.; VerCauteren, Kurt C.] USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Ft Collins, CO 80526 USA. [Spraker, Terry R.] Colorado State Univ, Coll Vet Med, Diagnost Lab, Ft Collins, CO 80523 USA. [Spraker, Terry R.] Colorado State Univ, Prion Res Ctr, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Kong, Qingzhong] Case Western Reserve Univ, Inst Pathol, Natl Prion Dis Pathol Surveillance Ctr, Cleveland, OH 44106 USA. RP Nichols, TA (reprint author), USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Ft Collins, CO 80526 USA. EM tracy.a.nichols@aphis.usda.gov NR 30 TC 1 Z9 1 U1 8 U2 17 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1933-6896 EI 1933-690X J9 PRION JI Prion PD SEP 3 PY 2015 VL 9 IS 5 BP 367 EP 375 DI 10.1080/19336896.2015.1086061 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CZ2DX UT WOS:000366916400007 PM 26636258 ER PT J AU Tang, JD Perkins, A Williams, WP Warburton, ML AF Tang, Juliet D. Perkins, Andy Williams, W. Paul Warburton, Marilyn L. TI Using genome-wide associations to identify metabolic pathways involved in maize aflatoxin accumulation resistance SO BMC GENOMICS LA English DT Article DE Host plant resistance; Pathway analysis; Genome-wide association analysis; Aflatoxin; Corn ID QUANTITATIVE TRAIT LOCI; ASPERGILLUS EAR ROT; JASMONIC ACID; CONTRIBUTING RESISTANCE; ENRICHMENT ANALYSIS; GENETIC-VARIATION; ARABIDOPSIS; DEFENSE; LIPOXYGENASE; EXPRESSION AB Background: Aflatoxin is a potent carcinogen that can contaminate grain infected with the fungus Aspergillus flavus. However, resistance to aflatoxin accumulation in maize is a complex trait with low heritability. Here, two complementary analyses were performed to better understand the mechanisms involved. The first coupled results of a genome-wide association study (GWAS) that accounted for linkage disequilibrium among single nucleotide polymorphisms (SNPs) with gene-set enrichment for a pathway-based approach. The rationale was that the cumulative effects of genes in a pathway would give insight into genetic differences that distinguish resistant from susceptible lines of maize. The second involved finding non-pathway genes close to the most significant SNP-trait associations with the greatest effect on reducing aflatoxin in multiple environments. Unlike conventional GWAS, the latter analysis emphasized multiple aspects of SNP-trait associations rather than just significance and was performed because of the high genotype x environment variability exhibited by this trait. Results: The most significant metabolic pathway identified was jasmonic acid (JA) biosynthesis. Specifically, there was at least one allelic variant for each step in the JA biosynthesis pathway that conferred an incremental decrease to the level of aflatoxin observed among the inbred lines in the GWAS panel. Several non-pathway genes were also consistently associated with lowered aflatoxin levels. Those with predicted functions related to defense were: leucine-rich repeat protein kinase, expansin B3, reversion-to-ethylene sensitivity1, adaptor protein complex2, and a multidrug and toxic compound extrusion protein. Conclusions: Our genetic analysis provided strong evidence for several genes that were associated with aflatoxin resistance. Inbred lines that exhibited lower levels of aflatoxin accumulation tended to share similar haplotypes for genes specifically in the pathway of JA biosynthesis, along with several non-pathway genes with putative defense-related functions. Knowledge gained from these two complementary analyses has improved our understanding of population differences in aflatoxin resistance. C1 [Tang, Juliet D.] USDA, FS Forest Prod Lab, Durabil & Wood Protect, Starkville, MS 39759 USA. [Williams, W. Paul; Warburton, Marilyn L.] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. RP Warburton, ML (reprint author), USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. EM marilyn.warburton@ars.usda.gov FU USDA; USAID; National Science Foundation [EPS-0903787] FX The authors are grateful to Chuan-Yu Hsu for her advice on molecular aspects of the project, the Cornell Institute for Genomic Diversity for their help in generating and analyzing the GBS data, and Gary Payne and Mike Kolomiets for critically reviewing the manuscript. This work was supported by the USDA and USAID Feed the Future. AP was supported by the National Science Foundation under grant EPS-0903787. Any mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA or USAID. NR 53 TC 3 Z9 3 U1 9 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD SEP 3 PY 2015 VL 16 AR 673 DI 10.1186/s12864-015-1874-9 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CQ4XE UT WOS:000360607100013 PM 26334534 ER PT J AU Wen, ZX Boyse, JF Song, QJ Cregan, PB Wang, DC AF Wen, Zixiang Boyse, John F. Song, Qijian Cregan, Perry B. Wang, Dechun TI Genomic consequences of selection and genome-wide association mapping in soybean SO BMC GENOMICS LA English DT Article DE Selective sweep; Single nucleotide polymorphisms; GWAS; Soybean ID MAP-BASED CLONING; AGRONOMIC TRAITS; QUANTITATIVE RESISTANCE; LINKAGE DISEQUILIBRIUM; ARABIDOPSIS-THALIANA; GENETIC DIVERSITY; GLYCINE-SOJA; INBRED LINES; POPULATION; WILD AB Background: Crop improvement always involves selection of specific alleles at genes controlling traits of agronomic importance, likely resulting in detectable signatures of selection within the genome of modern soybean (Glycine max L. Merr.). The identification of these signatures of selection is meaningful from the perspective of evolutionary biology and for uncovering the genetic architecture of agronomic traits. Results: To this end, two populations of soybean, consisting of 342 landraces and 1062 improved lines, were genotyped with the SoySNP50K Illumina BeadChip containing 52,041 single nucleotide polymorphisms (SNPs), and systematically phenotyped for 9 agronomic traits. A cross-population composite likelihood ratio (XP-CLR) method was used to screen the signals of selective sweeps. A total of 125 candidate selection regions were identified, many of which harbored genes potentially involved in crop improvement. To further investigate whether these candidate regions were in fact enriched for genes affected by selection, genome-wide association studies (GWAS) were conducted on 7 selection traits targeted in soybean breeding (grain yield, plant height, lodging, maturity date, seed coat color, seed protein and oil content) and 2 non-selection traits (pubescence and flower color). Major genomic regions associated with selection traits overlapped with candidate selection regions, whereas no overlap of this kind occurred for the non-selection traits, suggesting that the selection sweeps identified are associated with traits of agronomic importance. Multiple novel loci and refined map locations of known loci related to these traits were also identified. Conclusions: These findings illustrate that comparative genomic analyses, especially when combined with GWAS, are a promising approach to dissect the genetic architecture of complex traits. C1 [Wen, Zixiang; Boyse, John F.; Wang, Dechun] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Song, Qijian; Cregan, Perry B.] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Wang, DC (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St,Rm A384-E, E Lansing, MI 48824 USA. EM wangdech@msu.edu FU Michigan Soybean Promotion Committee FX We thank Dr. Frank Dennis for help in editing the manuscript. We also thank Nancy Boyse, Cuihua Gu, Ruijuan Tan and Jianzheng Yuan for technical assistance in experiment. Funding was provided by Michigan Soybean Promotion Committee. NR 52 TC 3 Z9 4 U1 10 U2 47 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD SEP 3 PY 2015 VL 16 AR 671 DI 10.1186/s12864-015-1872-y PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CQ4XE UT WOS:000360607100011 PM 26334313 ER PT J AU Yang, J Farmer, LM Agyekum, AAA Elbaz-Younes, I Hirschi, KD AF Yang, Jian Farmer, Lisa M. Agyekum, Abia A. A. Elbaz-Younes, Ismail Hirschi, Kendal D. TI Detection of an Abundant Plant-Based Small RNA in Healthy Consumers SO PLOS ONE LA English DT Article ID HUMAN PLASMA; MICRORNAS; DELIVERY; MIRNAS; CELLS; COMMUNICATION; BIOMARKERS; BACTERIA; VECTORS; PROTEIN AB The mechanisms of delivery of plant small RNAs to consumers must be investigated in order to harness this technology to positively impact biotechnology. Two groups have used honeysuckle (Lonicera japonica) feeding regimes to detect a plant-based small RNA, termed MIR2911, in sera. Meanwhile, numerous groups have failed to detect dietary plant-based small RNAs in consumers. Here we catalog levels of MIR2911 in different herbs, and suggest that in particular herb MIR2911 levels are elevated. Feeding these different herb-based diets to mice, we found MIR2911 levels in the sera and urine were associated with dietary intake levels. Abundance was not the sole determinate of apparent RNA bioavailability, as gavage-feeding large-doses of synthetic MIR2911 permitted only small transient increases in serum levels. Dietary MIR2911 were not modified in circulation by association with the host's RNA-induced silencing complex, as the RNA did not co-immunoprecipitate with AGO2. The stability of dietary MIR2911 in circulation differed from synthesized small RNAs, as tail vein administration of various synthetic plant-based small RNAs resulted in rapid clearance. However, synthetic MIR2911 appeared to be more stable than the other plant miRNAs tested. Notably, this uptake of dietary MIR2911 was not related to perturbations in the host's microbiome or gut permeability. We suggest dietary uptake of MIR2911 is commonplace in healthy consumers, and reproducible detection of plant-based small RNAs in consumers depends on dietary abundance, RNA stability and digestion from within the food-matrix. C1 [Yang, Jian; Farmer, Lisa M.; Agyekum, Abia A. A.; Elbaz-Younes, Ismail; Hirschi, Kendal D.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Hirschi, Kendal D.] Texas A&M Univ, Vegetable & Fruit Improvement Ctr, College Stn, TX USA. RP Hirschi, KD (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. EM kendalh@bcm.edu FU NIH [5T32HD071839-02]; USDA/ARIS [6250-51000-051-00D]; Soy Health Research Program; Dry Bean Health Research Program FX L.M.F. was supported by a grant from the NIH (5T32HD071839-02). This work was supported by USDA/ARIS 6250-51000-051-00D and funds from the Soy Health and Dry Bean Health Research Programs. NR 42 TC 7 Z9 8 U1 0 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 3 PY 2015 VL 10 IS 9 AR e0137516 DI 10.1371/journal.pone.0137516 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ5AJ UT WOS:000360615400064 PM 26335106 ER PT J AU Caicedo, JD Rivera-Vargas, LI Segarra, AE Davis, RE AF Caicedo, Jorge D. Rivera-Vargas, Lydia I. Segarra, Alejandro E. Davis, Robert E. TI Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico SO AUSTRALASIAN PLANT DISEASE NOTES LA English DT Article DE Pigeon pea witches' broom; Periwinkle; Nested PCR ID POLYMERASE-CHAIN-REACTION; GENE-SEQUENCES; RIBOSOMAL-RNA; WOODY-PLANTS; DISEASE; CLASSIFICATION; PCR; BRAZIL; DNA AB Few studies have determined the presence of phytoplasmas in important crops in Puerto Rico. Disease symptoms resembling those caused by phytoplasmas were observed in pigeon pea (Cajanus cajan), periwinkle (Catharanthus roseus), tabebuia (Tabebuia heterophylla), Spanish lime (Melicoccus bijugatus), ixora (Ixora coccinea), mango (Mangifera indica), cactus (Opuntia spp.), citrus trees (Citrus spp.), and coffee (Coffea arabica). Sixty-two samples from these species were tested using conventional PCR to amplify the 16S rRNA and ribosomal protein genes (rpIV-rpsC). Fifty-one percent of the tested samples (corresponding to periwinkle, pigeon pea, citrus, coffee and tabebuia) were positive for phytoplasmas, with amplicons of 0.8 (16S rRNA gene) and 1.2 kb (rpIV-rpsC genes), depending upon primers used in PCRs. For both genetic loci, DNA sequences showed 99 % identity with pigeon pea witches' broom phytoplasma (PPWB). Due to the lack of studies of potential insect vectors, common Auchenorrhyncha species were sweep-collected from pigeon pea and citrus and tested for phytoplasma. Of nine insect genera collected, Empoasca kraemeri (Cicadellidae), Melormenis antillarum (Flatidae), and Colpoptera maculifrons (Issidae) were positive for PPWB based on results from conventional PCR and DNA sequence analysis. The findings indicate that these insects fed upon the aforementioned plant species, ingesting contents of phloem, and may act as potential phytoplasma vectors in the field. These are first reports of PPWB phytoplasma infections in citrus species (C. sinensis and C. limon), coffee, periwinkle and tabebuia, and in insects (E. kraemeri, M. antillarum and C. maculifrons) for Puerto Rico. C1 [Caicedo, Jorge D.; Rivera-Vargas, Lydia I.] Univ Puerto Rico, Plant Pathol Lab, Coll Agr Sci, Dept Crops & Agroenvironm Sci, Mayaguez Campus, Mayaguez, PR USA. [Segarra, Alejandro E.] Univ Puerto Rico, Luis F Martorell Insectarium, Coll Agr Sci, Dept Crops & Agroenvironm Sci, Mayaguez Campus, Mayaguez, PR USA. [Davis, Robert E.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. RP Caicedo, JD (reprint author), Univ Puerto Rico, Plant Pathol Lab, Coll Agr Sci, Dept Crops & Agroenvironm Sci, Mayaguez Campus, Mayaguez, PR USA. EM jorge.caicedo@upr.edu; lydiai.rivera@upr.edu; alejandro.segarra@upr.edu; robert.davis@ars.usda.gov NR 36 TC 2 Z9 2 U1 1 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1833-928X J9 AUSTRALAS PLANT DIS JI Australas. Plant Dis. Notes PD SEP 2 PY 2015 VL 10 IS 1 AR 28 DI 10.1007/s13314-015-0178-x PG 8 WC Plant Sciences SC Plant Sciences GA DH7EQ UT WOS:000372955200001 ER PT J AU Gray, CA Runyon, JB Jenkins, MJ Giunta, AD AF Gray, Curtis A. Runyon, Justin B. Jenkins, Michael J. Giunta, Andrew D. TI Mountain Pine Beetles Use Volatile Cues to Locate Host Limber Pine and Avoid Non-Host Great Basin Bristlecone Pine SO PLOS ONE LA English DT Article ID DENDROCTONUS-PONDEROSAE COLEOPTERA; LODGEPOLE PINE; HOPKINS COLEOPTERA; SCOLYTIDAE; MONOTERPENES; ORIENTATION; INTEGRATION; PHEROMONES; RESPONSES; OUTBREAKS AB The tree-killing mountain pine beetle (Dendroctonus ponderosae Hopkins) is an important disturbance agent of western North American forests and recent outbreaks have affected tens of millions of hectares of trees. Most western North American pines (Pinus spp.) are hosts and are successfully attacked by mountain pine beetles whereas a handful of pine species are not suitable hosts and are rarely attacked. How pioneering females locate host trees is not well understood, with prevailing theory involving random landings and/or visual cues. Here we show that female mountain pine beetles orient toward volatile organic compounds (VOCs) from host limber pine (Pinus flexilis James) and away from VOCs of non-host Great Basin bristlecone pine (Pinus longaeva Bailey) in a Y-tube olfactometer. When presented with VOCs of both trees, females overwhelmingly choose limber pine over Great Basin bristlecone pine. Analysis of VOCs collected from co-occurring limber and Great Basin bristlecone pine trees revealed only a few quantitative differences. Noticeable differences included the monoterpenes 3-carene and D-limonene which were produced in greater amounts by host limber pine. We found no evidence that 3-carene is important for beetles when selecting trees, it was not attractive alone and its addition to Great Basin bristlecone pine VOCs did not alter female selection. However, addition of D-limonene to Great Basin bristlecone pine VOCs disrupted the ability of beetles to distinguish between tree species. When presented alone, D-limonene did not affect behavior, suggesting that the response is mediated by multiple compounds. A better understanding of host selection by mountain pine beetles could improve strategies for managing this important forest insect. Moreover, elucidating how Great Basin bristlecone pine escapes attack by mountain pine beetles could provide insight into mechanisms underlying the incredible longevity of this tree species. C1 [Gray, Curtis A.; Jenkins, Michael J.; Giunta, Andrew D.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Runyon, Justin B.] US Forest Serv, USDA, Rocky Mt Res Stn, Forestry Sci Lab, Bozeman, MT USA. RP Gray, CA (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. EM curtis.gray@aggiemail.usu.edu FU USFS EM Project [INT-EM-F-10-02]; Utah Agricultural Experiment Station (UAES) UTA Project [1070]; USDA National Needs Graduate Fellowship Competitive NIFA Grant; PECASE (President's Early Career Award in Science and Engineering) FX This project was supported by funding from USFS EM Project INT-EM-F-10-02, Utah Agricultural Experiment Station (UAES) UTA Project 1070, USDA National Needs Graduate Fellowship Competitive NIFA Grant, and PECASE (President's Early Career Award in Science and Engineering) to Justin Runyon. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 5 Z9 5 U1 5 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 2 PY 2015 VL 10 IS 9 AR e0135752 DI 10.1371/journal.pone.0135752 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ4ZT UT WOS:000360613800039 PM 26332317 ER PT J AU Pollegioni, P Woeste, KE Chiocchini, F Del Lungo, S Olimpieri, I Tortolano, V Clark, J Hemery, GE Mapelli, S Malvolti, ME AF Pollegioni, Paola Woeste, Keith E. Chiocchini, Francesca Del Lungo, Stefano Olimpieri, Irene Tortolano, Virginia Clark, Jo Hemery, Gabriel E. Mapelli, Sergio Malvolti, Maria Emilia TI Ancient Humans Influenced the Current Spatial Genetic Structure of Common Walnut Populations in Asia SO PLOS ONE LA English DT Article ID JUGLANS-REGIA L.; MULTIPLE-REGRESSION; CROP DOMESTICATION; DIVERSITY; HISTORY; TECHNOLOGY; SOFTWARE; PATTERNS; CAUCASUS; DISTANCE AB Common walnut (Juglans regia L) is an economically important species cultivated worldwide for its wood and nuts. It is generally accepted that J. regia survived and grew spontaneously in almost completely isolated stands in its Asian native range after the Last Glacial Maximum. Despite its natural geographic isolation, J. regia evolved over many centuries under the influence of human management and exploitation. We evaluated the hypothesis that the current distribution of natural genetic resources of common walnut in Asia is, at least in part, the product of ancient anthropogenic dispersal, human cultural interactions, and afforestation. Genetic analysis combined with ethno-linguistic and historical data indicated that ancient trade routes such as the Persian Royal Road and Silk Road enabled long-distance dispersal of J. regia from Iran and Trans-Caucasus to Central Asia, and from Western to Eastern China. Ancient commerce also disrupted the local spatial genetic structure of autochthonous walnut populations between Tashkent and Samarkand (Central-Eastern Uzbekistan), where the northern and central routes of the Northern Silk Road converged. A significant association between ancient language phyla and the genetic structure of walnut populations is reported even after adjustment for geographic distances that could have affected both walnut gene flow and human commerce over the centuries. Beyond the economic importance of common walnut, our study delineates an alternative approach for understanding how the genetic resources of long-lived perennial tree species may be affected by the interaction of geography and human history. C1 [Pollegioni, Paola; Chiocchini, Francesca; Olimpieri, Irene; Tortolano, Virginia; Malvolti, Maria Emilia] CNR, Inst Agroenvironm & Forest Biol, Porano, Terni, Italy. [Woeste, Keith E.] Purdue Univ, Dept Forestry & Nat Resources, US Forest Serv, USDA,Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Del Lungo, Stefano] CNR, Inst Archaeol & Monumental Heritage, Potenza, Italy. [Clark, Jo] Earth Trust, Abingdon, Oxon, England. [Hemery, Gabriel E.] Sylva Fdn, Little Wittenham, Oxon, England. [Mapelli, Sergio] CNR, Inst Agr Biol & Biotechnol, Milan, Italy. RP Pollegioni, P (reprint author), CNR, Inst Agroenvironm & Forest Biol, Porano, Terni, Italy. EM paola.pollegioni@ibaf.cnr.it FU European Community in the framework of the Seventh Framework Programme under the Marie Curie Actions COFUND project "I-MOVE" [267232, 206405] FX This study was supported by the European Community in the framework of the Seventh Framework Programme under the Marie Curie Actions COFUND project "I-MOVE" (N. 267232) Grant number 206405. NR 62 TC 4 Z9 4 U1 9 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD SEP 2 PY 2015 VL 10 IS 9 AR e0135980 DI 10.1371/journal.pone.0135980 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ4ZT UT WOS:000360613800046 PM 26332919 ER PT J AU Bermejo-Alvarez, P Ramos-Ibeas, P Park, KE Powell, AP Vansandt, L Derek, B Ramirez, MA Gutierrez-Adan, A Telugu, BP AF Bermejo-Alvarez, P. Ramos-Ibeas, P. Park, K. E. Powell, A. P. Vansandt, L. Derek, Bickhart Ramirez, M. A. Gutierrez-Adan, A. Telugu, B. P. TI Tet-mediated imprinting erasure in H19 locus following reprogramming of spermatogonial stem cells to induced pluripotent stem cells SO SCIENTIFIC REPORTS LA English DT Article ID DNA DEMETHYLATION; METHYLATION IMPRINT; WILMS-TUMOR; GERM-CELLS; MOUSE; GENES; MICE; 5-HYDROXYMETHYLCYTOSINE; GENERATION; EXPRESSION AB Selective methylation of CpG islands at imprinting control regions (ICR) determines the monoparental expression of a subset of genes. Currently, it is unclear whether artificial reprogramming induced by the expression of Yamanaka factors disrupts these marks and whether cell type of origin affects the dynamics of reprogramming. In this study, spermatogonial stem cells (SSC) that harbor paternalized imprinting marks, and fibroblasts were reprogrammed to iPSC (SSCiPSC and fiPSC). The SSCiPSC were able to form teratomas and generated chimeras with a higher skin chimerism than those derived from fiPSC. RNA-seq revealed extensive reprogramming at the transcriptional level with 8124 genes differentially expressed between SSC and SSCiPSC and only 490 between SSCiPSC and fiPSC. Likewise, reprogramming of SSC affected 26 of 41 imprinting gene clusters known in the mouse genome. A closer look at H19 ICR revealed complete erasure in SSCiPSC in contrast to fiPSC. Imprinting erasure in SSCiPSC was maintained even after in vivo differentiation into teratomas. Reprogramming of SSC from Tet1 and Tet2 double knockout mice however lacked demethylation of H19 ICR. These results suggest that imprinting erasure during reprogramming depends on the epigenetic landscape of the precursor cell and is mediated by TETs at the H19 locus. C1 [Bermejo-Alvarez, P.; Park, K. E.; Vansandt, L.; Telugu, B. P.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Bermejo-Alvarez, P.; Park, K. E.; Powell, A. P.; Telugu, B. P.] ARS, USDA, Anim Biosci & Biotechnol Lab, Beltsville, MD USA. [Derek, Bickhart] ARS, USDA, Anim Improvement Program Lab, Beltsville, MD USA. [Bermejo-Alvarez, P.; Ramos-Ibeas, P.; Ramirez, M. A.; Gutierrez-Adan, A.] INIA, Dept Anim Reprod, Madrid, Spain. RP Bermejo-Alvarez, P (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. EM borrillobermejo@hotmail.com; btelugu@umd.edu RI Gutierrez-Adan, Alfonso/A-1485-2014 OI Gutierrez-Adan, Alfonso/0000-0001-9893-9179 FU Department of Animal and Avian Sciences, University of Maryland; Maryland Agriculture Experimental Station (MAES); Maryland Stem Cell Research Fund (MSCRF); FPI grant [RYC-2012-10193]; Spanish Ministry of Economy and Innovation [AGL-2012-39652-CO2-01] FX We thank Drs. Le Ann Blomberg, Steven Schroeder, and Harry Dawson for assistance with preparation of RNA-Seq libraries, quality control and deep sequencing of samples. We thank the University of Maryland and the Biology Department for use of the Bioinformatics Core which provided the transcriptome analysis. BT is supported by the Department of Animal and Avian Sciences, University of Maryland, Maryland Agriculture Experimental Station (MAES), and Maryland Stem Cell Research Fund (MSCRF). PBA is supported by RYC-2012-10193, PRI by a FPI grant and AGA by AGL-2012-39652-CO2-01, from the Spanish Ministry of Economy and Innovation. NR 41 TC 1 Z9 2 U1 2 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD SEP 2 PY 2015 VL 5 AR 13691 DI 10.1038/srep13691 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CQ3YS UT WOS:000360541400001 PM 26328763 ER PT J AU Silva, CJ Vazquez-Fernandez, E Onisko, B Requena, JR AF Silva, Christopher J. Vazquez-Fernandez, Ester Onisko, Bruce Requena, Jesus R. TI Proteinase K and the structure of PrPSc: The good, the bad and the ugly SO VIRUS RESEARCH LA English DT Review DE Prion; PrPSc; PrPSc structure; Proteinase K; Limited proteolysis; beta-Solenoid ID SCRAPIE PRION PROTEIN; CREUTZFELDT-JAKOB-DISEASE; BOVINE SPONGIFORM ENCEPHALOPATHY; TRANSMISSIBLE MINK ENCEPHALOPATHY; WESTERN IMMUNOBLOTTING PROCEDURE; TRITIRACHIUM-ALBUM LIMBER; SMALL-MOLECULE REAGENTS; MASS-SPECTROMETRY; AMINO-ACID; LIMITED PROTEOLYSIS AB Infectious proteins (prions) are, ironically, defined by their resistance to proteolytic digestion. A defining characteristic of the transmissible isoform of the prion protein (PrPSc) is its partial resistance to proteinase K (PK) digestion. Diagnosis of prion disease typically relies upon immunodetection of PK-digested PrPSc by Western blot, ELISA or immunohistochemical detection. PK digestion has also been used to detect differences in prion strains. Thus, PK has been a crucial tool to detect and, thereby, control the spread of prions. PK has also been used as a tool to probe the structure of PrPSc. Mass spectrometry and antibodies have been used to identify PK cleavage sites in PrPSc. These results have been used to identify the more accessible, flexible stretches connecting the beta-strand components in PrPsc. These data, combined with physical constraints imposed by spectroscopic results, were used to propose a qualitative model for the structure of PrPSc. Assuming that PrPSc is a four rung beta-solenoid, we have threaded the PrP sequence to satisfy the PK proteolysis data and other experimental constraints. (C) 2015 Elsevier B.V. All rights reserved. C1 [Silva, Christopher J.] ARS, USDA, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. [Vazquez-Fernandez, Ester] Univ Alberta, Ctr Prions & Prot Folding Dis, Edmonton, AB T6G 2M8, Canada. [Onisko, Bruce] OniPro, Kensington, CA USA. [Requena, Jesus R.] Univ Santiago de Compostela, IDIS, CIMUS Biomed Res Inst, Santiago De Compostela 15782, Spain. RP Silva, CJ (reprint author), ARS, USDA, Produce Safety & Microbiol Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov; jesus.requena@usc.es OI Silva, Christopher/0000-0003-4521-6377 FU European Commission [FOOD-CT-2006-023183, 222887]; Spanish Ministry of Education and Science [BFU2006-04588/BMC] FX JRR was supported by FP7 grants FOOD-CT-2006-023183 (StrainBarrier) and 222887 (Priority) from the European Commission, and BFU2006-04588/BMC from the Spanish Ministry of Education and Science. We wish to acknowledge the assistance of Melissa Erickson-Beltran in preparing this manuscript and Joaquin Castilla for insightful discussion and assistance in elaborating Fig. 2. We thank Dr. Bruce Chesebro, Rocky Mountain Laboratory, NIH, Montana, USA, for the generous gift of GPI- mice. NR 99 TC 8 Z9 8 U1 3 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD SEP 2 PY 2015 VL 207 SI SI BP 120 EP 126 DI 10.1016/j.virusres.2015.03.008 PG 7 WC Virology SC Virology GA CP9YK UT WOS:000360251600013 PM 25816779 ER PT J AU Guo, MM Jin, TZ Yadav, MP Yang, RJ AF Guo, Mingming Jin, Tony Z. Yadav, Madhav P. Yang, Ruijin TI Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Micro-emulsion; Chitosan; Barley straw arabinoxylan; Allyl isothiocyanate; High pressure homogenization; Listeria ID PARTICLE-SIZE DISTRIBUTIONS; ESCHERICHIA-COLI O157-H7; ALLYL ISOTHIOCYANATE; GAMMA-IRRADIATION; ESSENTIAL OILS; GROUND-BEEF; CORN FIBER; SHELF-LIFE; CHITOSAN; COATINGS AB Edible antimicrobial composite films from micro-emulsions containing all natural compounds were developed and their antimicrobial properties and microstructures were investigated. Chitosan, allyl isothiocyanate (AIT), barley straw arabinoxylan (BSAX), and organic acids (acetic, lactic and levulinic acids) were used as film-forming agent, antimicrobial agent, emulsifier, and solvent, respectively. Micro-emulsions were obtained using high pressure homogenization (HPH) processing at 138 MPa for 3 cycles. The composite films made from the Micro-emulsions significantly (p < 0.05) inactivated Listeria innocua in tryptic soy broth (TSB) and on the surface of ready-to-eat (RTE) meat samples, achieving microbial reductions of over 4 log CFU/ml in TSB after 2 days at 22 degrees C and on meat samples after 35 days at 10 degrees C. AIT was a major contributor to the antimicrobial property of the films and HPH processing further enhanced its antimicrobial efficacy, while the increase of chitosan from 1.5% to 3%, or addition of acetic acid to the formulations didn't result in additional antimicrobial effects. This study demonstrated an effective approach to developing new edible antimicrobial films and coatings used for food applications. Published by Elsevier B.V. C1 [Guo, Mingming; Yang, Ruijin] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China. [Guo, Mingming; Jin, Tony Z.; Yadav, Madhav P.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Jin, TZ (reprint author), 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ars.usda.gov; rjyang2009@hotmail.com OI Jin, Tony/0000-0003-0504-5817 FU USDA-ARS CRIS project through ARS National Program 108 [1935-41420-092-00D]; Chinese Scholarship Council FX The authors wish to thank Anita Parameswaran, Stefanie Simon and Joseph Uknalis for their excellent technical assistance, and Dr. James Smith for his thoughtful review of this manuscript. This study was funded by the USDA-ARS CRIS project 1935-41420-092-00D through ARS National Program 108. Author Mingming Guo wishes to thank the Chinese Scholarship Council for financial support to work at ERRC-ARS-USDA. All work was done at ERRC-ARS-USDA. NR 55 TC 2 Z9 2 U1 3 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD SEP 2 PY 2015 VL 208 BP 58 EP 64 DI 10.1016/j.ijfoodmicro.2015.03.018 PG 7 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CN5KE UT WOS:000358467400008 PM 26057110 ER PT J AU Williams, MS Golden, NJ Ebel, ED Crarey, ET Tate, HP AF Williams, Michael S. Golden, Neal J. Ebel, Eric D. Crarey, Emily T. Tate, Heather P. TI Temporal patterns of Campylobacter contamination on chicken and their relationship to campylobacteriosis cases in the United States SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Campylobacteriosis; Times series; Seasonality; Retail chicken ID FOODBORNE ILLNESSES; HUMAN SALMONELLOSIS; OUTBREAK DATA; RAW CHICKEN; JEJUNI; RETAIL; SEASONALITY; POULTRY; QUANTIFICATION; IDENTIFICATION AB The proportion of Campylobacter contaminated food and water samples collected by different surveillance systems often exhibit seasonal patterns. In addition, the incidence of foodborne campylobacteriosis also tends to exhibit strong seasonal patterns. Of the various product classes, the occurrence of Campylobacter contamination can be high on raw poultry products, and chicken is often thought to be one of the leading food vehicles for campylobacteriosis. Two different federal agencies in the United States collected samples of raw chicken products and tested them for the presence of Campylobacter. During the same time period, a consortium of federal and state agencies operated a nationwide surveillance system to monitor cases of campylobacteriosis in the United States. This study uses a common modeling approach to estimate trends and seasonal patterns in both the proportion of raw chicken product samples that test positive for Campylobacter and cases of campylobacteriosis. The results generally support the hypothesis of a weak seasonal increase in the proportion of Campylobacter positive chicken samples in the summer months, though the number of Campylobacter on test-positive samples is slightly lower during this time period. In contrast, campylobacteriosis cases exhibit a strong seasonal pattern that generally precedes increases in contaminated raw chicken. These results suggest that while contaminated chicken products may be responsible for a substantial number of campylobacteriosis cases, they are most likely not the primary driver of the seasonal pattern in human illness. Published by Elsevier B.V. C1 [Williams, Michael S.; Golden, Neal J.; Ebel, Eric D.] USDA, Off Publ Hlth Sci, Food Safety & Inspect Serv, Ft Collins, CO 80526 USA. [Crarey, Emily T.; Tate, Heather P.] US FDA, Ctr Vet Med, Natl Antimicrobial Resistance Monitoring Syst, Laurel, MD 20708 USA. RP Williams, MS (reprint author), USDA, Off Publ Hlth Sci, Food Safety & Inspect Serv, 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM mike.williams@fsis.usda.gov NR 51 TC 4 Z9 4 U1 1 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD SEP 2 PY 2015 VL 208 BP 114 EP 121 DI 10.1016/j.ijfoodmicro.2015.05.018 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CN5KE UT WOS:000358467400014 PM 26065728 ER PT J AU Daimiel, L Ordovas, JM Davalos, A AF Daimiel, Lidia Ordovas, Jose Ma Davalos, Alberto TI miRNAs modified by dietary lipids in Caco-2 cells. A microarray screening SO GENOMICS DATA LA English DT Article DE MicroRNAs; Microarray screening; Clustering analyses; Dietary lipids; Caco-2 cells ID EXPRESSION AB We performed a screening of miRNAs regulated by dietary lipids in a cellular model of enterocytes, Caco-2 cells. Our aim was to describe new lipid-modified miRNAs with an implication in lipid homeostasis and cardiovascular disease [1,2]. For that purpose, we treated differentiated Caco-2 cells with micelles containing the assayed lipids (cholesterol, conjugated linoleic acid and docosahexaenoic acid) and the screening of miRNAs was carried out by microarray using the mu Paraflo (R) Microfluidic Biochip Technology of LC Sciences (Huston, TX, USA). Experimental design, microarray description and raw data have been made available in the GEO database with the reference number of GSE59153. Here we described in detail the experimental design and methods used to obtain the relative expression data. (C) 2015 The Authors. Published by Elsevier Inc. C1 [Daimiel, Lidia; Ordovas, Jose Ma; Davalos, Alberto] CEI UAM CSIC, IMDEA Food Inst, Nutr Genom Cardiovasc Dis & Obes, E-28049 Madrid, Spain. [Ordovas, Jose Ma] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. RP Daimiel, L (reprint author), CEI UAM CSIC, IMDEA Food Inst, Nutr Genom Cardiovasc Dis & Obes, E-28049 Madrid, Spain. EM lida.daimiel@mdea.org OI Davalos, Alberto/0000-0001-5709-6443 NR 4 TC 0 Z9 0 U1 2 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2213-5960 J9 GENOM DATA JI Genom. Data PD SEP PY 2015 VL 5 BP 171 EP 172 DI 10.1016/j.gdata.2015.05.011 PG 2 WC Genetics & Heredity SC Genetics & Heredity GA DO4MS UT WOS:000377757800051 PM 26484250 ER PT J AU Harrington, CA Carlson, WC AF Harrington, Constance A. Carlson, William C. TI Morphology and Accumulation of Epicuticular Wax on Needles of Douglas-fir (Pseudotsuga menziesii var. menziesii) SO NORTHWEST SCIENCE LA English DT Article DE Epicuticular wax; stomatal wax; morphology; Douglas-fir; Pseudotsuga menziesii var. menziesii; foliar fungi ID LEAF WAX; FINE-STRUCTURE; PICEA-PUNGENS; CONIFERS; RAINFALL; LEAVES; PLANTS AB Past studies have documented differences in epicuticular wax among several tree species but little attention has been paid to changes in accumulation of foliar wax that can occur during the year. We sampled current-year needles from the terminal shoots of Douglas-fir (Pseudotsuga menziesii var. menziesii) in late June/early July, late August and early November. Needles were sampled from two sites that differed in their climate and shoot phenology. Adaxial (upper), abaxial (lower) and cross-sectional surfaces were examined on scanning electron micrographs. Wax thickness increased significantly (P < 0.01) during the year (from 2.9 +/- 0.26 mu m in late June/early July to 4.4 +/- 0.13 mu m in early November). Mean wax thickness was slightly thicker on adaxial (4.0 +/- 0.16 mu m) than on abaxial (3.5 +/- 0.22 mu m) surfaces (P = 0.03). There were no significant differences in wax thickness between needles sampled at the base of the terminal shoot or near the tip of the shoot. Tubular or rod-shaped epicuticular wax crystals were sparsely developed on adaxial surfaces, completely covered abaxial surfaces (including filling all stomatal cavities), and had the same general structure and appearance across sites and sampling dates. Some erosion of epicuticular wax crystals on adaxial surfaces and presence of amorphous wax on abaxial surfaces was observed late in the year when epicuticular wax thickness was the thickest. Fungal hyphae were observed on top of epicuticular wax crystals and emerging from stomatal pores. C1 [Harrington, Constance A.; Carlson, William C.] US Forest Serv, USDA, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. RP Harrington, CA (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM charrington@fs.fed.us FU Oregon State office of the Bureau of Land Management FX Funding for this project was provided by the Oregon State office of the Bureau of Land Management. Scanning electron micrographs were taken by Ron Zarges, Weyerhaeuser Company, Federal Way, WA. We thank USFS J. Herbert Stone Nursery and Hancock Forest Management for access to the trees for sample collection, Peter Gould for image analysis programming, and Kevin Ford for data analysis. NR 31 TC 0 Z9 0 U1 2 U2 2 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD SEP PY 2015 VL 89 IS 4 BP 401 EP 408 PG 8 WC Ecology SC Environmental Sciences & Ecology GA DM8NH UT WOS:000376620400007 ER PT J AU Baumann, F Buck, BJ Metcalf, RV Mclaurin, BT Merkler, D Carbone, M AF Baumann, Francine Buck, Brenda J. Metcalf, Rodney V. Mclaurin, Brett T. Merkler, Doug Carbone, Michele TI Increased Proportion of Female and Young Mesothelioma Cases Are Indicators of Environmental Exposure to Carcinogenic Mineral Fibers in Nevada SO JOURNAL OF THORACIC ONCOLOGY LA English DT Meeting Abstract DE Mesothelioma; asbestos; Environmental epidemiology; Exposure indicators C1 [Baumann, Francine; Carbone, Michele] Univ Hawaii, Ctr Canc, Honolulu, HI 96822 USA. [Buck, Brenda J.; Metcalf, Rodney V.] Univ Nevada, Geosci, Las Vegas, NV 89154 USA. [Mclaurin, Brett T.] Bloomsburg Univ Penn, Dept Environm Geog & Geol Sci, Bloomsburg, PA 17815 USA. [Merkler, Doug] USDA, Nat Resources Conservat Serv, Las Vegas, NV USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1556-0864 EI 1556-1380 J9 J THORAC ONCOL JI J. Thorac. Oncol. PD SEP PY 2015 VL 10 IS 9 SU 2 MA ORAL23.05 BP S218 EP S219 PG 2 WC Oncology; Respiratory System SC Oncology; Respiratory System GA DE1CX UT WOS:000370365100342 ER PT J AU Maleki, SJ Teuber, SS Bublin, M Chruszcz, M Grimm, CC Cheng, H Hurlbur, BK Breiteneder, H Schein, C AF Maleki, S. J. Teuber, S. S. Bublin, M. Chruszcz, M. Grimm, C. C. Cheng, H. Hurlbur, B. K. Breiteneder, H. Schein, C. TI The epitopes that cause cross-reactions between peanuts and tree nuts SO ALLERGY LA English DT Meeting Abstract CT Congress of the European-Academy-of-Allergy-and-Clinical-Immunology CY JUN 06-10, 2015 CL Barcelona, SPAIN SP European Acad Allergy & Clin Immunol C1 [Maleki, S. J.] USDA, Food Allergy, New Orleans, LA USA. [Teuber, S. S.] Univ Calif Davis, Sch Med, Davis, CA USA. [Bublin, M.; Breiteneder, H.] Med Univ Vienna, Vienna, Austria. [Chruszcz, M.] Univ S Carolina, Columbia, SC 29208 USA. [Grimm, C. C.; Cheng, H.] USDA, Food Allergy, Agr Res Ctr, New Orleans, LA USA. [Hurlbur, B. K.; Schein, C.] Fdn Appl Mol Evolut, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0105-4538 EI 1398-9995 J9 ALLERGY JI Allergy PD SEP PY 2015 VL 70 SU 101 SI SI MA 307 BP 145 EP 145 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DD5GM UT WOS:000369950701086 ER PT J AU Hurlburt, B Cheng, H Offermann, L Chruszcz, M Santos, A Lack, G Maleki, S AF Hurlburt, B. Cheng, H. Offermann, L. Chruszcz, M. Santos, A. Lack, G. Maleki, S. TI Epitope mapping the peanut panallergen Ara h 8 SO ALLERGY LA English DT Meeting Abstract CT Congress of the European-Academy-of-Allergy-and-Clinical-Immunology CY JUN 06-10, 2015 CL Barcelona, SPAIN SP European Acad Allergy & Clin Immunol C1 [Hurlburt, B.; Cheng, H.; Maleki, S.] ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. [Offermann, L.; Chruszcz, M.] Univ S Carolina, Chem & Biochem, Columbia, SC 29208 USA. [Santos, A.; Lack, G.] Kings Coll London, Pediat Allergy, London WC2R 2LS, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0105-4538 EI 1398-9995 J9 ALLERGY JI Allergy PD SEP PY 2015 VL 70 SU 101 SI SI MA 1210 BP 493 EP 493 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DD5GM UT WOS:000369950702540 ER PT J AU Champlin, PJ Kilgo, JC Lanham, JD Spilker, FJ AF Champlin, Paul J. Kilgo, John C. Lanham, J. Drew Spilker, Frank J. TI Area Sensitivity of Grassland Sparrows Overwintering in a South Carolina Forested Landscape SO SOUTHEASTERN NATURALIST LA English DT Article ID DOUGLAS-FIR FORESTS; HABITAT ASSOCIATIONS; TALLGRASS PRAIRIE; POPULATION TRENDS; BACHMANS SPARROW; WINTER ABUNDANCE; PATCH SIZE; BIRDS; PATTERNS; FRAGMENTATION AB We assessed area sensitivity of overwintering Peucaea aestivalis (Bachman's Sparrow), Ammodramus savannarum (Grasshopper Sparrow), and A. henslowii (Henslow's Sparrow) within utility rights-of-way (ROWs) at the US Department of Energy's Savannah River Site (SRS) in South Carolina. We compared sparrow abundance among 4 ROW-width classes (25-44.9 m, 45-64.9 m, 65-84.9 m, and >= 85 m) and used landform index (LFI; a measure of topography and environmental exposure) as a covariate in our analyses to assess potential effects of abiotic characteristics. Total number of sparrows flushed/ha, Grasshopper Sparrows flushed/ha, and Henslow's Sparrows flushed/ha were positively related to ROW-width class. Total number of sparrows flushed/ha and Bachman's Sparrows flushed/ha were negatively related to LFI, indicating a positive relationship with site exposure. Utility ROWs in the Southeast provide wintering habitat for grassland sparrows, especially on exposed elevated plateaus within wide ROWs. C1 [Champlin, Paul J.; Lanham, J. Drew] Clemson Univ, Dept Forest Resources, Clemson, SC 29634 USA. [Kilgo, John C.] USDA Forest Serv, Southern Res Stn, POB 700, New Ellenton, SC 29809 USA. [Spilker, Frank J.] BST, Vicksburg, MS 39180 USA. RP Kilgo, JC (reprint author), USDA Forest Serv, Southern Res Stn, POB 700, New Ellenton, SC 29809 USA. EM jkilgo@fs.fed.us FU Clemson University; US Forest Service Southern Research Station; US Department of Energy-Savannah River Operations Office through the US Forest Service Savannah River [DE-AI09-00SR22188] FX We thank E. Summers, D. Jones, and S. Junker for their efforts in the field, and J. Blake and K. Wright for logistical assistance. We are indebted to M. Vukovich for assisting with GIS and creating maps. The manuscript was improved by the comments of 3 anonymous reviewers. Funding was provided by Clemson University, the US Forest Service Southern Research Station, and the US Department of Energy-Savannah River Operations Office through the US Forest Service Savannah River under Interagency Agreement DE-AI09-00SR22188. NR 40 TC 0 Z9 0 U1 2 U2 6 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD SEP PY 2015 VL 14 IS 3 BP 438 EP 446 DI 10.1656/058.014.0305 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NK UT WOS:000370892500003 ER PT J AU Edelman, AJ Moran, J Garrabrant, TJ Vorreiter, KC AF Edelman, Andrew J. Moran, John Garrabrant, Timothy J. Vorreiter, Kaley C. TI Muskrat Predation of Native Freshwater Mussels in Shoal Creek, Alabama SO SOUTHEASTERN NATURALIST LA English DT Article ID UNITED-STATES; UNIONIDAE; CLAM AB Widespread decline of native freshwater mussels has increased their susceptibility to extinction from environmental factors such as competition, disease, and predation. Mammals, in particular Ondatra zibethicus (Common Muskrat), are well documented as major predators of freshwater mussels. We assessed the impact of Common Muskrats on the native freshwater-mussel community in Shoal Creek, AL. We surveyed 12.7 km of Shoal Creek for signs of mussel predation by Common Muskrats. Based on the species composition of observed shell middens, Common Muskrats foraged on all 6 native mussel species in Shoal Creek, including federally listed threatened and endangered species (Hamiota altilis [Finelined Pocketbook] and Pleurobema georgianum [Southern Pigtoe], respectively). Common Muskrats appeared to feed on native mussels based on their natural availability rather than exhibiting strong size-selective predation. C1 [Edelman, Andrew J.; Garrabrant, Timothy J.; Vorreiter, Kaley C.] Univ West Georgia, Dept Biol, Carrollton, GA 30118 USA. [Moran, John] US Forest Serv, Natl Forests Alabama, Montgomery, AL 36107 USA. RP Edelman, AJ (reprint author), Univ West Georgia, Dept Biol, Carrollton, GA 30118 USA. EM aedelman@westga.edu NR 32 TC 0 Z9 0 U1 7 U2 10 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD SEP PY 2015 VL 14 IS 3 BP 473 EP 483 DI 10.1656/058.014.0307 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NK UT WOS:000370892500005 ER PT J AU Park, Y Stanley, D AF Park, Youngjin Stanley, David TI Physiological trade-off between cellular immunity and flight capability in the wing-dimorphic sand cricket, Gryllus firmus SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Insect immunity; Immunosenescence; Gryllus firmus; Nodulation; Phospholipase A(2); Wing dimorphism ID DROSOPHILA-MELANOGASTER; BACTERIAL-INFECTIONS; PHOSPHOLIPASE A(2); APIS-MELLIFERA; MANDUCA-SEXTA; INSECT; EICOSANOIDS; XENORHABDUS; DEFENSE; IMMUNOCOMPETENCE AB The sand cricket, Gryllus firmus, is a wing-dimorphic species with long-wing (LW) and short wing (LW) morphs. The LW forms have very well developed wings and flight muscles and their SW counterparts have reduced wings and flight muscles, coupled with greater resource allocations to reproduction. This is the well-known oogenesis-flight syndrome, in which resources are differentially allocated to reproduction or flight. Insects have also evolved very sophisticated and robust immune systems, understood as a major adaptation that partly accounts for the long-term biological success of insects. Nodulation is the predominant cellular immune reaction to microbial infections, responsible for clearing most infecting microbes from hemolymph circulation in the first two hours after an infection. This is a biologically expensive process because in large infections millions of hemocytes are invested into melanotic nodules. These lost hemocytes are later replaced in hematopoietic organs. Honey bees and a few other insect species redirect resources from cellular immunity to another biologically expensive activity, foraging flights. These reallocations are conceived as physiological trade-offs, in which the costs of one biological function are traded off for another. Based on previous work, we posed the hypothesis that the sand cricket, Gryllus firmus, already known to reallocate resources in the oogenesis-flight syndrome, also reallocates resources from immune functions to flight. Here, we report that relative to long-wing morphs, the short-wing forms have higher immune capacity, registered in terms of nodulation reactions to the same challenge, higher hemocytic phospholipase A(2) activity (an enzyme involved in immune signaling) and relatively reduced age-related immunosenescence. We infer from these data that sand crickets, and likely many insect species, reallocate resources between flight, reproduction and immunity. Such trade-offs may support other functions, such as foraging and mate finding, as well. (C) 2015 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved. C1 [Park, Youngjin] Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. [Stanley, David] USDA ARS, Biol Control Insect Res Lab, Columbia, MO 65203 USA. RP Park, Y (reprint author), Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. EM happy2pyj@gmail.com FU Korea Science & Engineering Foundation (KOSEF); Basic Science Research Program through NRF (National Research Foundation) of Korea - Ministry of Education [2013R1A1A2061353] FX This work was supported by the Post-doctoral Fellowship Program of Korea Science & Engineering Foundation (KOSEF) and Basic Science Research Program through NRF (National Research Foundation) of Korea funded by the Ministry of Education (2013R1A1A2061353) to YP. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 33 TC 0 Z9 0 U1 3 U2 10 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD SEP PY 2015 VL 18 IS 3 BP 553 EP 559 DI 10.1016/j.aspen.2015.07.007 PG 7 WC Entomology SC Entomology GA DC6SI UT WOS:000369349500031 ER PT J AU Sutter, RD Wainscott, SB Boetsch, JR Palmer, CJ Rugg, DJ AF Sutter, Robert D. Wainscott, Susan B. Boetsch, John R. Palmer, Craig J. Rugg, David J. TI Practical Guidance for Integrating Data Management into Long-Term Ecological Monitoring Projects SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE data management; graded approach; iterative design; long-term ecological monitoring; metadata; quality assurance ID HABITAT; QUALITY; PRECISION; SCIENCE AB Long-term monitoring and research projects are essential to understand ecological change and the effectiveness of management activities. An inherent characteristic of long-term projects is the need for consistent data collection over time, requiring rigorous attention to data management and quality assurance. Recent papers have provided broad recommendations for data management; however, practitioners need more detailed guidance and examples. We present general yet detailed guidance for the development of comprehensive, concise, and effective data management for monitoring projects. The guidance is presented as a graded approach, matching the scale of data management to the needs of the organization and the complexity of the project. We address the following topics: roles and responsibilities; consistent and precise data collection; calibration of field crews and instrumentation; management of tabular, photographic, video, and sound data; data completeness and quality; development of metadata; archiving data; and evaluation of existing data from other sources. This guidance will help practitioners execute effective data management, thereby, improving the quality and usability of data for meeting project objectives as well as broader meta-analysis and macrosystem ecology research. (C) 2015 The Authors. Wildlife Society Bulletin published by The Wildlife Society. C1 [Sutter, Robert D.] Enduring Conservat Outcomes, 652 Maupas Ave, Savannah, GA 31401 USA. [Wainscott, Susan B.] Univ Nevada, Univ Lib, Las Vegas, NV 89154 USA. [Boetsch, John R.] Natl Pk Serv, North Coast & Cascades Network Inventory & Monito, Port Angeles, WA 98362 USA. [Palmer, Craig J.] Univ Nevada, Sch Environm & Publ Affairs, Las Vegas, NV 89154 USA. [Palmer, Craig J.] Comp Sci Corp, Alexandria, VA 22310 USA. [Rugg, David J.] US Forest Serv, USDA, Madison, WI 53726 USA. RP Sutter, RD (reprint author), Enduring Conservat Outcomes, 652 Maupas Ave, Savannah, GA 31401 USA. EM rsutter@enduringconservation.com OI Wainscott, Susan/0000-0001-9994-0956; Rugg, David/0000-0003-2280-8302 NR 64 TC 5 Z9 5 U1 5 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD SEP PY 2015 VL 39 IS 3 BP 451 EP 463 DI 10.1002/wsb.548 PG 13 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0ER UT WOS:000368890500002 ER PT J AU Barker, NKS Fontaine, PC Cumming, SG Stralberg, D Westwood, A Bayne, EM Solymos, P Schmiegelow, FKA Song, SJ Rugg, DJ AF Barker, Nicole K. S. Fontaine, Patricia C. Cumming, Steven G. Stralberg, Diana Westwood, Alana Bayne, Erin M. Solymos, Peter Schmiegelow, Fiona K. A. Song, Samantha J. Rugg, David J. TI Ecological Monitoring Through Harmonizing Existing Data: Lessons from the Boreal Avian Modelling Project SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE Boreal Avian Modelling Project; breeding bird survey; data management; density estimation; detection correction; ecological monitoring; North America; point-count surveys; population size estimation; species distribution models ID BIRD COUNTS; CONSERVATION; DIVERSITY; EVOLUTION; RESPONSES; PATTERNS; CLIMATE; CANADA AB To accomplish the objectives of a long-term ecological monitoring program (LTEM), repurposing research data collected by other researchers is an alternative to original data collection. The Boreal Avian Modelling (BAM) Project is a 10-year-old project that has integrated the data from >100 avian point-count studies encompassing thousands of point-count surveys, and harmonized across data sets to account for heterogeneity induced by methodological and other differences. The BAM project faced the classic data-management challenges any LTEM must deal with, as well as special challenges involved with harmonizing so many disparate data sources. We created a data system consisting of 4 components: Archive (to preserve each contributor's data), Avian Database (harmonized point-count data), Biophysical Database (spatially explicit environmental covariates), and Software Tools library (linking the other components and providing analysis capability). This system has allowed the project to answer many questions about boreal birds; we believe it to be successful enough to merit consideration for use in monitoring other taxa. We have learned a number of lessons that will guide the project as it moves forward. These include the importance of creating a data protocol, the critical importance of high-quality metadata, and the need for a flexible design that accommodates changes in field techniques. One of the challenges the BAM team faced-gaining access to relevant data sets-may become easier with the increased expectation by journals and funding agencies that documenting and preserving research data be a standard part of scientific research. (C) 2015 The Wildlife Society. C1 [Barker, Nicole K. S.; Fontaine, Patricia C.] Univ Alberta, Boreal Avian Modelling Project, Edmonton, AB T6G 2H1, Canada. [Cumming, Steven G.] Univ Laval, Dept Sci Bois & Foret, Quebec City, PQ G1V 0A6, Canada. [Stralberg, Diana; Bayne, Erin M.; Solymos, Peter] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada. [Westwood, Alana] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4R2, Canada. [Solymos, Peter] Univ Alberta, Alberta Biodivers Monitoring Inst, Edmonton, AB T6G 2E9, Canada. [Schmiegelow, Fiona K. A.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2H1, Canada. [Song, Samantha J.] Environm Canada, Canadian Wildlife Serv, Prairie & Northern Reg, Edmonton, AB T6B 1K5, Canada. [Rugg, David J.] US Forest Serv, USDA, Res & Dev, Madison, WI 53726 USA. RP Barker, NKS (reprint author), Univ Alberta, Boreal Avian Modelling Project, Edmonton, AB T6G 2H1, Canada. EM nbarker@ualberta.ca OI Schmiegelow, Fiona/0000-0002-8219-8684; Stralberg, Diana/0000-0003-4900-024X; Rugg, David/0000-0003-2280-8302 FU Environment Canada; U.S. Fish & Wildlife Service FX This publication is a contribution of the Boreal Avian Modelling (BAM) Project, which is an international research collaboration for the ecology, management, and conservation of boreal birds. We acknowledge the BAM Project's data partners, funding agencies, including Environment Canada and U.S. Fish & Wildlife Service, and Technical Committee members who made this project possible, listed in full at www.borealbirds.ca/index.php/acknowledgements. We also thank the associate editor and two anonymous reviewers for their valuable comments on earlier versions of this paper. NR 45 TC 1 Z9 1 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD SEP PY 2015 VL 39 IS 3 BP 480 EP 487 DI 10.1002/wsb.567 PG 8 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0ER UT WOS:000368890500005 ER PT J AU Jachowski, DS Katzner, T Rodrigue, JL Ford, WM AF Jachowski, David S. Katzner, Todd Rodrigue, Jane L. Ford, W. Mark TI Monitoring Landscape-Level Distribution and Migration Phenology of Raptors Using a Volunteer Camera-Trap Network SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE Aquila chrysaetos; camera trap; citizen science; golden eagle; landscape; migration; monitoring; raptor ID WIND-ENERGY DEVELOPMENT; GOLDEN EAGLES; COMPETITIVE INTERACTIONS; POPULATION TRENDS; AQUILA-CHRYSAETOS; CITIZEN SCIENCE; TURKEY VULTURES; LONG-TERM; BIRDS; CHALLENGES AB Conservation of animal migratory movements is among the most important issues in wildlife management. To address this need for landscape-scale monitoring of raptor populations, we developed a novel, baited photographic observation network termed the "Appalachian Eagle Monitoring Program" (AEMP). During winter months of 2008-2012, we partnered with professional and citizen scientists in 11 states in the United States to collect approximately 2.5 million images. To our knowledge, this represents the largest such camera-trap effort to date. Analyses of data collected in 2011 and 2012 revealed complex, often species-specific, spatial and temporal patterns in winter raptor movement behavior as well as spatial and temporal resource partitioning between raptor species. Although programmatic advances in data analysis and involvement are needed, the continued growth of the program has the potential to provide a long-term, cost-effective, range-wide monitoring tool for avian and terrestrial scavengers during the winter season. Perhaps most importantly, by relying heavily on citizen scientists, AEMP has the potential to improve long-term interest and support for raptor conservation and serve as a model for raptor conservation programs in other portions of the world. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Jachowski, David S.] Clemson Univ, Dept Forestry & Environm Conservat, Clemson, SC 29631 USA. [Katzner, Todd] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID 83706 USA. [Rodrigue, Jane L.] US Forest Serv, No Res Stn, Princeton, WV 24740 USA. [Ford, W. Mark] US Geol Survey, Virginia Cooperat Fish & Wildlife Res Unit, Blacksburg, VA 24061 USA. [Ford, W. Mark] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. RP Jachowski, DS (reprint author), Univ KwaZulu Natal, Sch Life Sci, Durban, South Africa. EM djachowski@gmail.com OI Katzner, Todd/0000-0003-4503-8435 FU West Virginia University Division of Forestry; U.S. Department of Agriculture Forest Service Northern Research Station FX We thank the hundreds of volunteers who helped carry out this project and continue to carry it forward into the future. A group of > 250 volunteers and agency personnel contributed to data collection for this project. They are acknowledged in the Appalachian Eagles Annual Report, available from TEK. In addition, T. Miller, K. O'Malley, K. Heyden, S. Somershoe, C. Todd, C. Martin, T. Salo, C. Kelly, D. Cuthrell, D. Brinker, E. Soehren, and S. Allen assisted with coordination of the camera-trapping effort. Financial support was provided by the West Virginia University Division of Forestry and the U.S. Department of Agriculture Forest Service Northern Research Station. Finally, we thank C. Jachowski for advice on quantitative analysis. The use of any trade, product, or firm names in this manuscript does not imply endorsement by the U.S. government. We thank R. Jachowski, the Associate Editor, and two anonymous reviewers for their constructive comments on earlier drafts of this manuscript. NR 41 TC 2 Z9 2 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD SEP PY 2015 VL 39 IS 3 BP 553 EP 563 DI 10.1002/wsb.571 PG 11 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0ER UT WOS:000368890500014 ER PT J AU Lonsinger, RC Gese, EM Waits, LP AF Lonsinger, Robert C. Gese, Eric M. Waits, Lisette P. TI Evaluating the Reliability of Field Identification and Morphometric Classifications for Carnivore Scats Confirmed with Genetic Analysis SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE Canis latrans; classification trees; fecal DNA; misidentification; noninvasive genetic sampling; scat deposition; scat identification; species identification; Vulpes macrotis ID BLACK-BACKED JACKALS; GREAT-BASIN DESERT; BAT-EARED FOXES; SWIFT FOX; SPECIES IDENTIFICATION; VULPES-VELOX; CAPE FOXES; GRAY FOX; COYOTE; FECES AB Scat surveys are commonly used to monitor carnivore populations. Scats of sympatric carnivores can be difficult to differentiate and field-based identification can be misleading. We evaluated the success of field-based species identification for scats of 2 sympatric carnivores-coyotes (Canis latrans) and kit foxes (Vulpes macrotis). We conducted scat surveys in the Great Basin desert of Utah, USA, during the winter and summer of 2013, and we detected 1,680 carnivore scats. We classified scats based on field identification, recorded morphometric measurements, and collected fecal DNA samples for molecular species identification. We subsequently evaluated the classification success of field identification and the predictive power of 2 nonparametric classification techniques-k-nearest neighbors and classification trees-based on scat measurements. Overall, 12.2% of scats were misclassified by field identification, but misclassifications were not equitable between species. Only 7.1% of the scats identified as coyote with field identification were misclassified, compared with 22.9% of scats identified as kit fox. Results from both k-nearest neighbor and classification-tree analyses suggest that morphometric measurements provided an objective alternative to field identification that improved classification of rarer species. Overall misclassification rates for k-nearest neighbor and classification-tree analyses were 11.7% and 7.5%, respectively. Using classification trees, misclassification was reduced for kit foxes (8.5%) and remained similar for coyotes (7.2%), relative to field identification. Although molecular techniques provide unambiguous species identification, classification approaches may offer a cost-effective alternative. We recommend that monitoring programs employing scat surveys utilize molecular species identification to develop training data sets and evaluate the accuracy of field-based and statistical classification approaches. (C) 2015 The Wildlife Society. C1 [Lonsinger, Robert C.; Waits, Lisette P.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Gese, Eric M.] Utah State Univ, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv,Dept Wildland Resources, Logan, UT 84322 USA. RP Lonsinger, RC (reprint author), 875 Perimeter Dr MS1136, Moscow, ID 83844 USA. EM Lons1663@vandals.uidaho.edu FU U.S. Department of Defense's Environmental Security Technology Certification Program [12 EB-RC5-006]; Legacy Resource Management Program [W9132T-12-2-0050]; National Geographic Society's Conservation Trust [C248-13] FX Funding support was provided by the U.S. Department of Defense's Environmental Security Technology Certification Program (12 EB-RC5-006) and Legacy Resource Management Program (W9132T-12-2-0050) and by the National Geographic Society's Conservation Trust (C248-13). The U.S. Army Dugway Proving Ground Natural Resource Management Program provided logistical support. The Utah Division of Wildlife Resources provided housing. We thank B. Kluever, E. Burke, K. Cobb, J. Decotis, T. Edwards, C. Perkins, and M. Richmond for assistance with field work. J. Adams provided laboratory guidance and support. L. Gardiner, R. Irwin, T. Lasley, K. LeWallen, A. Lundgren, K. Miller, A. Reisenauer, and P. Stinebaugh assisted with DNA extractions. We thank C. Ribic, L. Berkman, L. Prugh, and an anonymous reviewer for their helpful comments on an earlier version of the manuscript. NR 53 TC 4 Z9 4 U1 9 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD SEP PY 2015 VL 39 IS 3 BP 593 EP 602 DI 10.1002/wsb.549 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0ER UT WOS:000368890500018 ER PT J AU Lavelle, MJ Blass, CR Fischer, JW Hygnstrom, SE Hewitt, DG VerCauteren, KC AF Lavelle, Michael J. Blass, Chad R. Fischer, Justin W. Hygnstrom, Scott E. Hewitt, David G. VerCauteren, Kurt C. TI Food Habits of Adult Male White-Tailed Deer Determined by Camera Collars SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE camera; feeding habits; food selection; Odocoileus virginianus; white-tailed deer ID SOUTH TEXAS-PLAINS; VIDEO-CAMERA; DIETS; SELECTION; VEGETATION AB Many factors influence what and why animals select the foods they eat. Several methods have been used to estimate food habits of herbivores, but they all have limitations such as defining available foods and misrepresenting particular forages. We evaluated plant consumption by adult male white-tailed deer (Odocoileus virginianus) with camera collars in a semi-enclosed population in southern Texas, USA, during late autumn 2010. We collected 1,241 videos taken at all times of the day and night from 15 camera collars and confirmed consumption of 40 plant species with an 84% probability of identification. Diets of individual deer varied considerably, though there was an apparent preference for prickly-pear cactus (Opuntia engelmannii) and blackbrush acacia (Vachellia rigidula). Our results were consistent with other conventional studies of food habits of deer in the region. Yet, we feel camera collars provide a more thorough and detailed representation of forage species available and consumed. Strategic deployments of camera collars could advance the understanding of nutritional requirements and behavior of deer because a broad array of concurrent data can be collected. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Lavelle, Michael J.; Blass, Chad R.; Fischer, Justin W.; VerCauteren, Kurt C.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Hygnstrom, Scott E.] Univ Wisconsin, Coll Nat Resources, Stevens Point, WI 54481 USA. [Hewitt, David G.] Texas A&M Univ, Caesar Kleberg Wildlife Res Inst, Kingsville, TX 78363 USA. RP Lavelle, MJ (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM michael.j.lavelle@aphis.usda.gov FU USDA-APHIS-Wildlife Services-National Wildlife Research Center FX We thank C. Anderson, G. Clements, C. Currie, C. Ellis, L. Gulick, M. McCollum, C. Minor, R. Moll, P. Nol, R. Pleszewski, T. Ruby, J. Rhyan, G. Schuster, L. Soliz, G. Clements, and U.S. Department of Agriculture-Animal and Plant Health Inspection Service (USDA-APHIS)-Veterinary Services for assisting throughout the study. Special thanks to A. Hildreth for assisting with various aspects and responsibilities. We thank the Caesar Kleberg Wildlife Research Institute at Texas A&M University-Kingsville for logistical support and the USDA-APHIS-Wildlife Services-National Wildlife Research Center for financial support. Editorial comments provided by T. Boal, C. Ribic, M. Hewison, N. Snow, and two anonymous reviewers improved this article and are greatly appreciated. Mention of companies or commercial products does not imply recommendation or endorsement by the USDA, nor does omission imply criticism. Product names are mentioned solely to report factually on available equipment and to provide specific information. There are no known conflicts of interest associated with this publication and there has been no significant financial support for this work influencing its outcome. NR 37 TC 0 Z9 0 U1 11 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD SEP PY 2015 VL 39 IS 3 BP 651 EP 657 DI 10.1002/wsb.556 PG 7 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0ER UT WOS:000368890500025 ER PT J AU Ryman, VE Pighetti, GM Lippolis, JD Gandy, JC Applegate, CM Sordillo, LM AF Ryman, V. E. Pighetti, G. M. Lippolis, J. D. Gandy, J. C. Applegate, C. M. Sordillo, L. M. TI Quantification of bovine oxylipids during intramammary Streptococcus uberis infection SO PROSTAGLANDINS & OTHER LIPID MEDIATORS LA English DT Article DE Oxylipids; Inflammation; Mastitis; Eicosanoid; Cyclooxygenase; Lipoxygenase ID POLYUNSATURATED FATTY-ACIDS; NF-KAPPA-B; ENDOTHELIAL-CELLS; LIPID MEDIATORS; LINOLEIC-ACID; IN-VIVO; INFLAMMATORY RESPONSE; TRANSCRIPTION FACTOR; EPITHELIAL-CELLS; GENE-EXPRESSION AB Streptococcus uberis mastitis results in severe mammary tissue damage in dairy cows due to uncontrolled inflammation. Oxylipids are potent lipid mediators that orchestrate pathogen-induced inflammatory responses, however, changes in oxylipid biosynthesis during S. uberis mastitis are unknown. Thus, the current objective was to determine how oxylipid concentrations change in milk and mammary tissues during different stages of S. uberis mastitis. Increased arachidonic acid and linoleic acid-derived oxylipids were significantly increased in S. uberis-infected bovine mammary tissue. Linoleic acid metabolites, hydroxyoctadecadienoic acid (MODE) and oxooctadecadienoic acid, predominated in tissue and milk. Furthermore, in vitro exposure of bovine mammary endothelial cells to 13-hydroperoxyoctadecadienoic acid, upstream metabolite of HODE, significantly increased cyclooxygenase-2 expression, but 13-HODE exposure had no effect. The findings in the current study indicate lipidomic profiling may explain some of the dynamics of inflammation during bacterial challenge, however continued research is necessary to determine sample compartments which best reflect disease pathogenesis. (c) 2015 Elsevier Inc. All rights reserved. C1 [Ryman, V. E.; Gandy, J. C.; Applegate, C. M.; Sordillo, L. M.] Michigan State Univ, Coll Vet Med, E Lansing, MI 48824 USA. [Pighetti, G. M.] Univ Tennessee, Dept Anim Sci, Knoxville, IN 37996 USA. [Lippolis, J. D.] USDA ARS, Periparturient Dis Cattle Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Sordillo, LM (reprint author), Michigan State Univ, Coll Vet Med, Dept Large Anim Clin Sci, E Lansing, MI 48824 USA. EM sordillo@msu.edu FU Agriculture and Food Research Initiative Competitive Grants Programs of the USDA National Institute for Food and Agriculture [2011-67015-30179, 2015-67011-23012]; Matilda R. Wilson Fund (Detroit, MI) FX The authors acknowledge the resources and staff of the Michigan State University Research Technology Support Facility Mass Spectrometry Core, especially Drs. Dan Jones and Scott Smith. This work was funded, in part, through grants (2011-67015-30179 and 2015-67011-23012) from the Agriculture and Food Research Initiative Competitive Grants Programs of the USDA National Institute for Food and Agriculture and by an endowment from the Matilda R. Wilson Fund (Detroit, MI). NR 59 TC 2 Z9 3 U1 1 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1098-8823 EI 2212-196X J9 PROSTAG OTH LIPID M JI Prostaglandins Other Lipid Mediat. PD SEP PY 2015 VL 121 BP 207 EP 217 DI 10.1016/j.prostaglandins.2015.09.006 PN B PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA DA2OS UT WOS:000367636300009 PM 26432060 ER PT J AU Srivastava, PK O'Neill, P Cosh, M Kurum, M Lang, R Joseph, A AF Srivastava, Prashant K. O'Neill, Peggy Cosh, Michael Kurum, Mehmet Lang, Roger Joseph, Alicia TI Evaluation of Dielectric Mixing Models for Passive Microwave Soil Moisture Retrieval Using Data From ComRAD Ground-Based SMAP Simulator SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article; Proceedings Paper CT 13th Specialist Meeting on Microwave Radiometry and Remote Sensing of the Environment (MicroRad) CY MAR 24-27, 2014 CL Pasadena, CA SP Inst Elect & Elect Engineers, IEEE Geoscience & Remote Sensing Soc DE Combined radar/radiometer (ComRAD); dielectric models; L band; single-channel algorithm (SCA); soil moisture ID SOUTHERN GREAT-PLAINS; SURFACE-ROUGHNESS; SMOS SATELLITE; WATER-CONTENT; WET SOIL; RADIOMETRY; EMISSION; BEHAVIOR; MISSION; RADAR AB Soil moisture measurements are required to improve our understanding of hydrological processes and linkages between the Earth's water, energy, and carbon cycles. The efficient retrieval of soil moisture depends on various factors among which soil dielectric mixing models are considered to be an important factor. The main objective of this work focuses on testing different dielectric models-Mironov et al., Dobson et al., Wang and Schmugge, and Hallikainen et al.-for soil moisture retrieval using the combined radar/radiometer (ComRAD) ground-based L-band simulator system, which serves as a simulator for the instruments on NASA's soil moisture active passive (SMAP) mission scheduled for launch in early next year. The single-channel algorithm at H polarization (SCA-H) version of the tau-omega model was used for soil moisture retrieval. A summer field experiment was conducted in 2012 at the United States Department of Agriculture (USDA) test site from which ComRAD measurements and validation samples of soil moisture were collected using theta probes and in situ sensors. The highest performance statistics combination in terms of high correlation (r), low root-mean-square error (RMSE), and least bias has been obtained with SCA-H using the Mironov dielectric model (r = 0.79; RMSE = 0.04 m(3)/m(3); bias = 0.01) followed by Dobson (r = 0.76; RMSE = 0.04 m(3)/m(3), bias = -0.01), Wang and Schmugge (r = 0.79; RMSE = 0.04 m(3)/m(3), bias = 0.02) and Hallikainen (r = 0.76; RMSE = 0.06 m(3)/m(3), bias = 0.04). Although the performance of the four dielectric models is relatively comparable, this analysis indicates that the Mironov dielectric model is marginally better than others for passive-only microwave soil moisture retrieval and could be a useful choice for SMAP satellite soil moisture retrieval. C1 [Srivastava, Prashant K.; O'Neill, Peggy; Joseph, Alicia] NASA, Goddard Space Flight Ctr, Hydrol Sci Lab, Greenbelt, MD 20771 USA. [Srivastava, Prashant K.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Cosh, Michael] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Kurum, Mehmet] TUBITAK BILGEM, Inst Informat Technol, TR-41470 Gebze, Kocaeli, Turkey. [Lang, Roger] George Washington Univ, Dept Elect & Comp Engn, Washington, DC 20052 USA. RP Srivastava, PK (reprint author), NASA, Goddard Space Flight Ctr, Hydrol Sci Lab, Greenbelt, MD 20771 USA. OI Cosh, Michael/0000-0003-4776-1918 NR 42 TC 2 Z9 2 U1 7 U2 13 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD SEP PY 2015 VL 8 IS 9 SI SI BP 4345 EP 4354 DI 10.1109/JSTARS.2014.2372031 PG 10 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA CZ8AW UT WOS:000367323300013 ER PT J AU Sheng, Y Ball, E Nossal, K AF Sheng, Yu Ball, Eldon Nossal, Katerina TI Comparing Agricultural Total Factor Productivity between Australia, Canada, and the United States, 1961-2006 SO INTERNATIONAL PRODUCTIVITY MONITOR LA English DT Article ID SUPERLATIVE INDEX NUMBERS; GROWTH; AGGREGATION; QUALITY; INPUT AB This article provides a comparison of levels and growth of agricultural total factor productivity between Australia, Canada, and the United States for the 1961-2006 period. A production account for agriculture that is consistent across the three countries is constructed to estimate output, input and total factor productivity, and a dynamic panel regression is used to link the productivity estimates to potential determinants. We show that investment in public research and development and infrastructure plays an important role in explaining differences in productivity levels between countries. The findings provide useful insights into how public policy could be used to sustain agricultural productivity growth. C1 [Sheng, Yu] Australian Dept Agr, Agr & Resource Econ & Sci ABARES, Canberra, ACT, Australia. [Ball, Eldon] USDA, Econ Res Serv, Washington, DC USA. RP Sheng, Y (reprint author), Australian Dept Agr, Agr & Resource Econ & Sci ABARES, Canberra, ACT, Australia. EM yu.sheng@agriculture.gov.au; eball@ers.usda.gov; katarina.nossal@gmail.com NR 46 TC 0 Z9 0 U1 3 U2 4 PU CENTRE STUDY LIVING STANDARDS PI OTTAWA PA 111 SPARKS ST, STE 500, OTTAWA, ON K1P 5B5, CANADA SN 1492-9759 EI 1492-9767 J9 INT PRODUCT MONIT JI Int. Product. Monit. PD FAL PY 2015 VL 29 BP 38 EP 59 PG 22 WC Economics SC Business & Economics GA CZ7PM UT WOS:000367291400003 ER PT J AU Jin, YF Goulden, ML Faivre, N Veraverbeke, S Sun, FP Hall, A Hand, MS Hook, S Randerson, JT AF Jin, Yufang Goulden, Michael L. Faivre, Nicolas Veraverbeke, Sander Sun, Fengpeng Hall, Alex Hand, Michael S. Hook, Simon Randerson, James T. TI Identification of two distinct fire regimes in Southern California: implications for economic impact and future change SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE fire regime; economic impact; fuel management; climate change; Santa Ana winds ID NORTHERN BAJA-CALIFORNIA; LAKE TAHOE BASIN; CLIMATE-CHANGE; WILDLAND FIRES; UNITED-STATES; WILDFIRE; EXPENDITURES; TEMPERATURE; REANALYSIS; SHIFTS AB The area burned by Southern California wildfires has increased in recent decades, with implications for human health, infrastructure, and ecosystem management. Meteorology and fuel structure are universally recognized controllers of wildfire, but their relative importance, and hence the efficacy of abatement and suppression efforts, remains controversial. Southern California's wildfires can be partitioned by meteorology: fires typically occur either during Santa Ana winds (SA fires) in October through April, or warm and dry periods in June through September (non-SA fires). Previous work has not quantitatively distinguished between these fire regimes when assessing economic impacts or climate change influence. Here we separate five decades of fire perimeters into those coinciding with and without SA winds. The two fire types contributed almost equally to burned area, yet SA fires were responsible for 80% of cumulative 1990-2009 economic losses ($3.1 Billion). The damage disparity was driven by fire characteristics: SA fires spread three times faster, occurred closer to urban areas, and burned into areas with greater housing values. Non-SA fires were comparatively more sensitive to age-dependent fuels, often occurred in higher elevation forests, lasted for extended periods, and accounted for 70% of total suppression costs. An improved distinction of fire type has implications for future projections and management. The area burned in non-SA fires is projected to increase 77% (+/- 43%) by the mid-21st century with warmer and drier summers, and the SA area burned is projected to increase 64% (+/- 76%), underscoring the need to evaluate the allocation and effectiveness of suppression investments. C1 [Jin, Yufang] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Jin, Yufang; Goulden, Michael L.; Faivre, Nicolas; Veraverbeke, Sander; Randerson, James T.] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA USA. [Sun, Fengpeng; Hall, Alex] Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA USA. [Hand, Michael S.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Hook, Simon] CALTECH, Jet Prop Lab, Pasadena, CA USA. RP Jin, YF (reprint author), Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. EM yujin@ucdavis.edu RI Hook, Simon/D-5920-2016; Hall, Alex/D-8175-2014; Veraverbeke, Sander/H-2301-2012; Goulden, Michael/B-9934-2008; OI Hook, Simon/0000-0002-0953-6165; Veraverbeke, Sander/0000-0003-1362-5125; Goulden, Michael/0000-0002-9379-3948 FU NASA [NNX10AL14G]; Jenkins Family Foundation FX This research was supported by NASA grant NNX10AL14G and the Jenkins Family Foundation. We are also grateful to Dr H Safford for his comments and Drs K Gebert and D Sapsis for their help with acquiring and interpreting the economic data. NR 44 TC 4 Z9 4 U1 11 U2 27 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD SEP PY 2015 VL 10 IS 9 AR 094005 DI 10.1088/1748-9326/10/9/094005 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CZ5KJ UT WOS:000367141000008 ER PT J AU Shapiro-Ilan, DI Hazir, S Lete, L AF Shapiro-Ilan, David I. Hazir, Selcuk Lete, Luis TI Viability and Virulence of Entomopathogenic Nematodes Exposed to Ultraviolet Radiation SO JOURNAL OF NEMATOLOGY LA English DT Article DE biocontrol; entomopathogenic nematode; Heterorhabditis; Steinernema; tolerance; ultraviolet ID APPLICATION TECHNOLOGY; STEINERNEMA-KUSHIDAI; TOLERANCE; INFECTIVITY; PESTS AB Entomopathogenic nematodes (EPNs) can be highly effective biocontrol agents, but their efficacy can be reduced due to exposure to environmental stress such as from ultraviolet (UV) radiation. Our objectives were to 1) compare UV tolerance among a broad array of EPN species, and 2) investigate the relationship between reduced nematode viability (after exposure to -17V) and virulence. Nematodes exposed to a UV radiation (254 nm) for 10 or 20 min were assessed separately for viability (survival) and virulence to Galleria mellonella. We compared 9 different EPN species and 15 strains: Heteratitabditis bactetiophora (Baffle; fl11, Oswego, and VS strains), H. floridensis (332), H. georgiana (Kesha), H. indica (HOM1), H. megidis (UK211), Steinernema carpocap.syte (All, Cxrd, DD136, and Sal strains), S. feltiae (SN), S. rarum (17C&E), 31nd S riobrave (355). In viability assessments, steinernematids, jx1rticularly sir of S. carpocqpsae, generally exhibited superior UV tolerance compared with the heterothabditids. However, some heterorhabditids tended to be more tolerant than others; e.g., H, megidis and H bactaiophom (Baffle) were most susceptible and H. bacteriophara (Vs) was the only heterorhabditid that did not exhibit a significant effect after 10 min of exposure. All heterorhabditids experienced reduced viability after 20 min exposure though several S. carpocapsetestrains did not. In total, after 10 or 20 min exposure, the viability of seven nematode strains did not differ from their non-UV exposed controls. In virulence assays; steinernematids (particularly S. carpocapsae strains) also tended to exhibit higher UV tolerance. However, in contrast to the viability measurements, all nematodes experienced a reduction in virulence relative to their controls. Correlation analysis revealed that viability among nematode strains is not necessarily related to virulence. In conclusion, our results indicate that the impact of UV varies substantially among EPNs; and viability alone is not a sufficient measure for potential impact on biocontrol efficacy as other characters such as virulence may be severely affected even when viability remains high. C1 [Shapiro-Ilan, David I.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Hazir, Selcuk] Adnan Menderes Univ, Fac Arts & Sci, Dept Biol, Aydin, Turkey. [Lete, Luis] APTA, Inst Biol, BR-13001970 Campinas, SP, Brazil. RP Shapiro-Ilan, DI (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. EM david.shapiro@ars.usda.gov NR 26 TC 1 Z9 1 U1 1 U2 3 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 184 EP 189 PG 6 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400004 PM 26527839 ER PT J AU Kokalis-Burelle, N AF Kokalis-Burelle, Nancy TI Pasteuria penetrans for Control of Meloidogyne incognita on Tomato and Cucumber, and M. arenaria on Snapdragon SO JOURNAL OF NEMATOLOGY LA English DT Article DE Antirrhinum majus; biological control; Cucumis sativus; cucumber; Meloidogvne; Pasteuria penetrans; Solanum lycopersicum; root-knot nematodes; snapdragon; tomato ID BELONOLAIMUS-LONGICAUDATUS; BIOLOGICAL-CONTROL; ENDOSPORES; SUSCEPTIBILITY; SUPPRESSION AB Meloidogyne incognita and Meloidogyne arenana are important parasitic nematodes of vegetable and ornamental crops. Microplot and greenhouse experiments were conducted to test commercial formulations of the biocontrol agent Pasteuria penetrans for control of M. incognita on tomato and cucumber and M. arenaria on snapdragon. Three methods of application for P. penetrans were assessed including seed, transplant, and post-plant treatments. Efficacy in controlling galling and reproduction of the two root-knot nematode species was evaluated. Seed treatment application was assessed only for M. incognita on cucumber-. Pasteuria treatment rates of a granular transplant formulation ranged from 1.5 X 10(5) endospores/cm(3) to 3 X 10(5) endospores/cm(3) of transplant mix applied at seeding. Additional applications of 1.5 X 10(5) endospores/cm(3) of soil were applied as a liquid formulation to soil post-transplant for both greenhouse and microplot trials. In greenhouse cucumber trials, all Past-curia treatments were equivalent to steamed soil for reducing M. incognita populations in roots and soil, and reducing nematode reproduction and galling. In cucumber microplot trials there were no differences among treatments for M. incognita populations in roots or soil, eggs/g root, or root condition ratings. Nematode reproduction on cucumber was low with Telone II and with the seed treatment plus post-plant application of Pasteuria, which had the lowest nematode reproduction. However: galling for all Pasteuria treatments was higher than galling with Telone II. Root-knot nematode control with Pasteuria in greenhouse and microplot trials varied on tomato and snapdragon. Positive results were achieved for control of AL incognita with the seed treatment application on cucumber. C1 [Kokalis-Burelle, Nancy] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Kokalis-Burelle, N (reprint author), USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM nancy.burelle@ars.usda.gov FU IR-4 Biopesticide Grants Program FX The author acknowledges the IR-4 Biopesticide Grants Program for providing funding, as well as Jackie Markle and Greg Hess for their technical support. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 19 TC 2 Z9 2 U1 4 U2 12 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 207 EP 213 PG 7 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400007 PM 26527842 ER PT J AU Beacham, J Thomas, S Schroeder, J Holland, L Morris, E Schmidt, N Murray, L Solano-Campos, F Hanson, S Eisenback, JD AF Beacham, J. Thomas, S. Schroeder, J. Holland, L. Morris, E. Schmidt, N. Murray, L. Solano-Campos, F. Hanson, S. Eisenback, J. D. TI HOST STATUS OF A NEW MELOIDOGYNE SPECIES FOUND PARASITIZING YELLOW AND PURPLE NUTSEDGES. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Beacham, J.; Thomas, S.; Morris, E.; Hanson, S.] New Mexico State Univ, Dept Entomol Plant Pathol & Weed Sci, Las Cruces, NM 88003 USA. [Schroeder, J.] USDA, Off Pest Management Policy, Washington, DC 20250 USA. [Holland, L.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Schmidt, N.] New Mexico State Univ, Econ Appl Stat & Int Business Dept, Las Cruces, NM 88003 USA. [Murray, L.] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [Solano-Campos, F.] Univ Nacl, Escuela Ciencias Biol, San Jose, Costa Rica. [Eisenback, J. D.] Virginia Tech, Dept Plant Pathol, Blacksburg, VA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 223 EP 223 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400023 ER PT J AU Blackburn, D Crawford, B Shapiro-Ilan, DI Adams, BJ AF Blackburn, D. Crawford, B. Shapiro-Ilan, D. I. Adams, B. J. TI ENVIRONMENTAL DRIVERS OF TRAIT CHANGES IN PHOTORHABDUS LUMINESCENS. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Blackburn, D.; Crawford, B.; Adams, B. J.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Shapiro-Ilan, D. I.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RI Adams, Byron/C-3808-2009 OI Adams, Byron/0000-0002-7815-3352 NR 0 TC 0 Z9 0 U1 0 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 224 EP 225 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400027 ER PT J AU Brown, AMV Howe, DK Peetz, AB Phillips, WS Zasada, IA Denver, DR AF Brown, A. M., V Howe, D. K. Peetz, A. B. Phillips, W. S. Zasada, I. A. Denver, D. R. TI GENOME SKIMMING: NOVEL INSIGHTS INTO PLANT-PARASITIC NEMATODE GENOMES AND POTENTIAL AVENUES FOR MANAGEMENT. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Brown, A. M., V; Howe, D. K.; Denver, D. R.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Peetz, A. B.; Phillips, W. S.; Zasada, I. A.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 226 EP 226 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400031 ER PT J AU Dandurand, LM Bryant, G Block, V De Jong, W Denver, D Hutchinson, P Jones, J Kuhl, J Knudsen, G Mimee, B Novy, R Thorton, M Wang, X Whitworth, J Zasada, I AF Dandurand, L-M Bryant, G. Block, V. De Jong, W. Denver, D. Hutchinson, P. Jones, J. Kuhl, J. Knudsen, G. Mimee, B. Novy, R. Thorton, M. Wang, X. Whitworth, J. Zasada, I. TI RISK ASSESSMENT AND ERADICATION OF GLOBODERA SPP. IN US PRODUCTION OF POTATO SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Dandurand, L-M; Hutchinson, P.; Kuhl, J.; Knudsen, G.; Thorton, M.] Univ Idaho, Moscow, ID 83844 USA. [Bryant, G.; Block, V.; Jones, J.] James Hutton Inst, Dundee, Scotland. [De Jong, W.] Cornell Univ, Ithaca, NY 14853 USA. [Denver, D.] Oregon State Univ, Corvallis, OR 97331 USA. [Mimee, B.] Agr & Agri Food Canada, St Jean, PQ J3B 3E6, Canada. [Novy, R.; Wang, X.; Whitworth, J.; Zasada, I.] USDA ARS, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 230 EP 230 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400041 ER PT J AU Davies, KA Giblin-Davis, RM Ye, W Scheffer, S Taylor, GS AF Davies, K. A. Giblin-Davis, R. M. Ye, W. Scheffer, S. Taylor, G. S. TI TRI-TROPHIC CO-EVOLUTION IN THE FERGUSOBIA/FERGUSONINA/MYRTACEAE - A YARN FROM DOWN UNDER. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Davies, K. A.; Taylor, G. S.] Univ Adelaide, Adelaide, SA 5005, Australia. [Giblin-Davis, R. M.] Univ Florida, Davie, FL USA. [Ye, W.] North Carolina Dept Agr & Consumer Serv, Raleigh, NC USA. [Scheffer, S.] USDA ARS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 232 EP 232 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400046 ER PT J AU Davis, RF Knoll, JE Anderson, WF Harris-Shultz, KR AF Davis, R. F. Knoll, J. E. Anderson, W. F. Harris-Shultz, K. R. TI TOLERANCE OF SWEET SORGHUM TO MELOIDOGYNE INCOGNITA AND CROP EFFECT ON NEMATODE POPULATION DENSITY. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Davis, R. F.; Knoll, J. E.; Anderson, W. F.; Harris-Shultz, K. R.] USDA ARS, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 4 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 232 EP 232 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400047 ER PT J AU Handoo, ZA Carta, LK Skantar, AM Subbotin, SA Fraedrich, SW AF Handoo, Z. A. Carta, L. K. Skantar, A. M. Subbotin, S. A. Fraedrich, S. W. TI REDESCRIPTION AND MOLECULAR CHARACTERIZATION OF XIPHINEMA CHAMBERSI THORNE, 1939 (NEMATODA: LONGIDORIDAE) FROM LIVE OAK TREES IN JEKYLL ISLAND, GEORGIA WITH COMMENTS ON ITS MORPHOMETRIC VARIATIONS. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Handoo, Z. A.; Carta, L. K.; Skantar, A. M.] ARS, Nematol Lab, USDA, Beltsville, MD 20705 USA. [Subbotin, S. A.] Calif Dept Food & Agr, Plant Pest Diagnost Ctr, Sacramento, CA 95832 USA. [Fraedrich, S. W.] US Forest Serv, Southern Res Stn, Athens, GA 30602 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 242 EP 243 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400073 ER PT J AU Ibrahim, IKA Handoo, ZA AF Ibrahim, I. K. A. Handoo, Z. A. TI SURVEY OF PHYTOPARASITIC NEMATODES ASSOCIATED WITH SOME CROP PLANTS IN NORTHERN EGYPT. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Ibrahim, I. K. A.] Univ Alexandria, Fac Agr, Dept Plant Pathol, Alexandria, Egypt. [Handoo, Z. A.] ARS, Nematol Lab, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 247 EP 247 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400084 ER PT J AU Ingham, R Phillips, WS Peetz, A Wade, NM Zasada, IA AF Ingham, R. Phillips, W. S. Peetz, A. Wade, N. M. Zasada, I. A. TI EFFECTS OF THE CYST NEMATODE GLOBODERA ELLINGTONAE ON POTATO. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Ingham, R.; Wade, N. M.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Phillips, W. S.; Peetz, A.; Zasada, I. A.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 247 EP 247 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400085 ER PT J AU Kokalis-Burelle, N Hong, J Butler, DM Rosskopf, EN AF Kokalis-Burelle, N. Hong, J. Butler, D. M. Rosskopf, E. N. TI ANAEROBIC SOIL DISINFESTATION (ASD) COMBINED WITH SOIL SOLARIZATION FOR ROOT-KNOT NEMATODE CONTROL IN VEGETABLE AND ORNAMENTAL CROPS IN FLORIDA. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Kokalis-Burelle, N.; Hong, J.; Rosskopf, E. N.] ARS, USDA, Hort Res Lab, Ft Pierce, FL 34945 USA. [Butler, D. M.] Univ Tennessee, Knoxville, TN 37996 USA. NR 0 TC 0 Z9 0 U1 6 U2 8 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 251 EP 251 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400094 ER PT J AU Liu, C Timper, P Mengistu, TM Joseph, S AF Liu, C. Timper, P. Mengistu, T. M. Joseph, S. TI INFLUENCE OF ROOT EXUDATES ON ATTACHMENT OF PASTEURIA PENETRANS TO MELOIDOGYNE ARENARIA SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Liu, C.; Timper, P.] ARS, USDA, Tifton, GA 31793 USA. [Mengistu, T. M.; Joseph, S.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 252 EP 253 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400099 ER PT J AU Masler, EP Chitwood, DJ AF Masler, E. P. Chitwood, D. J. TI THE HETERODERA GLYCINES CYST AS A SOURCE OF NATURAL PRODUCTS: PROTEASES AND THEIR INHIBITION. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Masler, E. P.; Chitwood, D. J.] ARS, Nematol Lab, USDA, NEA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 254 EP 255 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400104 ER PT J AU Morris, K Langston, D Dickson, D Davis, R Timper, P Noe, J AF Morris, K. Langston, D. Dickson, D. Davis, R. Timper, P. Noe, J. TI EFFICACY OF FLUENSULFONE IN A TOMATO-CUCUMBER DOUBLE CROPPING SYSTEM. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Morris, K.; Langston, D.; Noe, J.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Dickson, D.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Davis, R.; Timper, P.] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 257 EP 258 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400110 ER PT J AU Myers, R Mello, C Keith, L AF Myers, R. Mello, C. Keith, L. TI SCREENING EDIBLE GINGER AND TURMERIC CULTIVARS FOR RESISTANCE TO ROOT-KNOT NEMATODES SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Myers, R.; Mello, C.; Keith, L.] ARS, USDA, DKI PBARC, Hilo, HI 96720 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 258 EP 258 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400112 ER PT J AU Phillips, WS Brown, AMV Howe, DK Peetz, AB Denver, DR Zasada, IA AF Phillips, W. S. Brown, A. M. V. Howe, D. K. Peetz, A. B. Denver, D. R. Zasada, I. A. TI THE MITOCHONDRIAL GENOME OF GLOBODERA ELLINGTONAE IS DIVIDED INTO TWO CIRCLES WITH BOTH IDENTICAL AND UNIQUE SEQUENCE REGIONS. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Phillips, W. S.; Peetz, A. B.; Zasada, I. A.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Brown, A. M. V.; Howe, D. K.; Denver, D. R.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 263 EP 263 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400124 ER PT J AU Phillips, WS Kieran, SR Kitner, M Zasada, IA AF Phillips, W. S. Kieran, S. R. Kitner, M. Zasada, I. A. TI TEMPERATURE AND DEVELOPMENTAL DYNAMICS OF GLOBODERA ELLINGTONAE. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Phillips, W. S.; Kieran, S. R.; Kitner, M.; Zasada, I. A.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 263 EP 263 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400125 ER PT J AU Showmaker, KC Sanders, WS Arick, MA Magbanua, ZV Peterson, DG Wubben, MJ AF Showmaker, K. C. Sanders, W. S. Arick, M. A., II Magbanua, Z. V. Peterson, D. G. Wubben, M. J. TI PROGRESS TOWARDS A ROTYLENCHULUS RENIFORMIS GENOME ASSEMBLY. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Showmaker, K. C.; Sanders, W. S.; Arick, M. A., II; Magbanua, Z. V.; Peterson, D. G.] Mississippi State Univ, Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39762 USA. [Showmaker, K. C.; Wubben, M. J.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Sanders, W. S.] Mississippi State Univ, Dept Comp Sci & Engn, Mississippi State, MS 39762 USA. [Peterson, D. G.; Wubben, M. J.] Mississippi State Univ, Dept Plant & Soil Sci, Mississippi State, MS 39762 USA. [Wubben, M. J.] ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 267 EP 268 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400138 ER PT J AU Skantar, AM Li, W Yan, Z Nakhla, MK AF Skantar, A. M. Li, W. Yan, Z. Nakhla, M. K. TI DETECTION AND IDENTIFICATION OF ANGUINA FUNESTA, A. AGROSTIS, A. TRITICI, AND A. PACIFICAE BY REAL-TIME PCR. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 ARS, Nematol Lab, USDA, Beltsville, MD 20705 USA. Anim & Plant Hlth Inspect Serv, USDA, Ctr Plant Hlth Sci & Technol, Beltsville Lab,USDA APHIS PPQ CPHST, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 268 EP 268 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400140 ER PT J AU Soumi, J Schmidt, LM Timper, P Hewlett, T Watrin, C Mekete, T AF Soumi, J. Schmidt, L. M. Timper, P. Hewlett, T. Watrin, C. Mekete, T. TI DETECTING GENOTYPIC VARIATION AMONG THE SINGLE SPORE ISOLATES OF PASTEURIA PENETRANS POPULATION OCCURRING IN FLORIDA USING SNP-BASED MARKERS. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Soumi, J.; Mekete, T.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Schmidt, L. M.; Hewlett, T.; Watrin, C.] Syngenta Crop Protect LLC, Greensboro, NC USA. [Timper, P.] ARS, USDA, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 269 EP 269 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400143 ER PT J AU Stetina, SR AF Stetina, S. R. TI DEVELOPMENT OF ROTYLENCHULUS RENIFORMIS DIFFERS ON RESISTANT GOSSYPIUM BARABDENSE ACCESSIONS TX 110 AND GB 713. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Stetina, S. R.] ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 270 EP 270 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400146 ER PT J AU Thies, J Harrison, HF Buckner, S AF Thies, J. Harrison, H. F. Buckner, S. TI NEMATODE-RESISTANT COVER CROP COWPEA FOR MANAGING ROOT-KNOT NEMATODES IN SUBSEQUENTLY PLANTED VEGETABLE CROPS. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Thies, J.] ARS, USDA, Jackson, TN 38301 USA. [Harrison, H. F.; Buckner, S.] ARS, USDA, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 5 U2 6 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 272 EP 272 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400151 ER PT J AU Timper, P Liu, C AF Timper, P. Liu, C. TI NATURAL SUPPRESSION OF MELOIDOGYNE INCOGNITA BY PASTEURIA PENETRANS IN COTTON. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Timper, P.] ARS, USDA, Tifton, GA 31793 USA. [Liu, C.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 273 EP 273 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400152 ER PT J AU Vieira, P Wantoch, S Eisenback, JD Kamo, K AF Vieira, P. Wantoch, S. Eisenback, J. D. Kamo, K. TI INSIGHT INTO THE SOYBEAN TRANSCRIPTIONAL PROFILING UPON INFECTION BY ROOT LESION NEMATODE. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Vieira, P.; Eisenback, J. D.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Vieira, P.; Wantoch, S.; Kamo, K.] ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 274 EP 274 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400155 ER PT J AU Wang, X Chen, S Zhang, S De Jong, WS Mitchum, MG AF Wang, X. Chen, S. Zhang, S. De Jong, W. S. Mitchum, M. G. TI CLE SIGNALING IN THE COMPATIBLE INTERACTION BETWEEN POTATO AND POTATO CYST NEMATODES. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Wang, X.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Wang, X.; Chen, S.; De Jong, W. S.] Cornell Univ, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Zhang, S.] Cornell Univ, Inst Biotechnol & Life Sci Technol, Prote & Mass Spectrometry Facil, Ithaca, NY 14853 USA. [Mitchum, M. G.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Mitchum, M. G.] Univ Missouri, Bond Life Sci Ctr, Columbia, MO 65211 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 275 EP 276 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400159 ER PT J AU Wen, Y Chitwood, DJ Meyer, SLF AF Wen, Y. Chitwood, D. J. Meyer, S. L. F. TI INHIBITORY EFFECTS OF GLEDITSIA SINENSIS (CHINESE HONEYLOCUST) POWDERED FRUITS AND EXTRACTS ON MELOIDOGYNE INCOGNITA. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Wen, Y.] South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou 510642, Guangdong, Peoples R China. [Chitwood, D. J.; Meyer, S. L. F.] ARS, Nematol Lab, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 276 EP 276 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400160 ER PT J AU Zasada, I Walters, TW Kroese, DR AF Zasada, I Walters, T. W. Kroese, D. R. TI HIDING PLACES FOR PRATYLENCHUS PENETRANS IN RASPBERRY. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Zasada, I] USDA ARS, Corvallis, OR 97330 USA. [Walters, T. W.] Walters Ag Res, Anacortes, WA 98221 USA. [Kroese, D. R.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 280 EP 281 PG 2 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400171 ER PT J AU Zasada, I Weiland, JE Global, L AF Zasada, I Weiland, J. E. Global, L. TI DIRECT APPLICATION OF ENERGY TO SOIL FOR THE MANAGEMENT OF SOILBORNE PATHOGENS AND PLANT-PARASITIC NEMATODES. SO JOURNAL OF NEMATOLOGY LA English DT Meeting Abstract C1 [Zasada, I; Weiland, J. E.] USDA ARS, Corvallis, OR 97330 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD SEP PY 2015 VL 47 IS 3 BP 280 EP 280 PG 1 WC Zoology SC Zoology GA CZ2DD UT WOS:000366914400169 ER PT J AU Ihekweazu, FD Ajjarapu, A Kellermayer, R AF Ihekweazu, Faith D. Ajjarapu, Avanthi Kellermayer, Richard TI Diagnostic Yield of Routine Enteropathogenic Stool Tests in Pediatric Ulcerative Colitis SO ANNALS OF CLINICAL AND LABORATORY SCIENCE LA English DT Article ID INFLAMMATORY-BOWEL-DISEASE; CLOSTRIDIUM-DIFFICILE INFECTION; GASTROENTERITIS; RISK; PATHOGEN AB Goals. It can be important to exclude infectious etiologies prior to adjusting immunosuppressive therapy in patients with ulcerative colitis (UC) exacerbation. We sought to determine the diagnostic yield of routine infectious stool studies in pediatric UC patients. Procedures. We conducted a retrospective review of 152 pediatric UC patients at Texas Children's Hospital between January 2003 and December 2009. The patient records were followed through July 2014. The number and type of infectious stool studies performed and the results of those were collected. Results. Three hundred fifty-four diagnostic stool tests were conducted for Clostridium difficile; 13.6% were positive. Two hundred twenty stool bacterial cultures were performed, and 1.8% were positive, all growing non-typhoid Salmonella. One of 13 (7.7%) Adenovirus PCR tests was positive. Two of 152 examinations (1.3%) for Ova and Parasites were positive. No stool tests for viral culture, viral particles, Yersinia or Rotavirus were positive. Conclusions. Clostridium difficile infection is common in pediatric UC, and routine screening during flares is strongly recommended. Other bacterial and parasitic infections routinely tested for are uncommon, but Salmonella may be a potentially important attribute to disease exacerbations in select patients. In patients without co-morbid conditions, the utility of performing non-specific fecal viral tests is questionable. C1 [Ihekweazu, Faith D.; Ajjarapu, Avanthi; Kellermayer, Richard] Texas Childrens Hosp, Baylor Coll Med, Sect Pediat Gastroenterol, USDA ARS,Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Ihekweazu, FD (reprint author), Baylor Coll Med, Sect Pediat Gastroenterol Hepatol & Nutr, 6621 Fannin St,CC1010-00, Houston, TX 77030 USA. EM faith.ihekweazu@bcm.edu FU NIH [T32 DK007664-24S1]; Gutsy Kids Fund; Karen and Brock Wagner family; Houston Men of Distinction FX FDI was supported by grant NIH T32 DK007664-24S1; RK was funded by philanthropic support from the Gutsy Kids Fund supported by the Karen and Brock Wagner family; and the Houston Men of Distinction. For the remaining authors none were declared. Funding sources had no role in the study design, in the collection, analysis and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication. NR 18 TC 0 Z9 0 U1 0 U2 0 PU ASSOC CLINICAL SCIENTISTS PI MIDDLEBURY PA PO BOX 1287, MIDDLEBURY, VT 05753 USA SN 0091-7370 EI 1550-8080 J9 ANN CLIN LAB SCI JI Ann. Clin. Lab. Sci. PD FAL PY 2015 VL 45 IS 6 BP 639 EP 642 PG 4 WC Medical Laboratory Technology SC Medical Laboratory Technology GA CY7UW UT WOS:000366615800005 PM 26663793 ER PT J AU Vandemark, G Guy, SO Chen, W McPhee, K Pfaff, J Lauver, M Muehlbauer, FJ AF Vandemark, G. Guy, S. O. Chen, W. McPhee, K. Pfaff, J. Lauver, M. Muehlbauer, F. J. TI Registration of 'Nash' Chickpea SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID RABIEI AB The kabuli chickpea (Cicer arientinum L.) cultivar Nash (Reg. No. CV-310; PI 673003) was released by the USDA-ARS in 2013 on the basis of its high yield and large seed size compared with the popular commercial chickpea cultivars Sierra and Sawyer. Nash is an F-5-derived line from the cross HB-19/CA9783142 and was evaluated in yield trials from 2008 to 2013 across 30 location-years in Idaho and Washington. Nash had similar performance as Sierra and Sawyer for several important agronomic traits, including days to 50% flowering, days to maturity, and canopy height. Nash also exhibited moderate resistance to Ascochyta blight, caused by Ascochyta rabiei (Pass.) Labrousse, which was similar to the disease reactions of Sierra and Sawyer. The mean yield of Nash over all trials was 1825 kg ha(-1), 17.2 and 4.5% higher than the mean yields of Sierra and Sawyer, respectively. Perhaps as important as its higher yield is that seed size of Nash was greater than Sierra and Sawyer. Two different measures of seed size were used: (i) weight of 100 seeds, and (ii) percentage of seeds with a diameter >9.1 mm. The value of a chickpea crop is influenced by the size of the harvested seed, with large seeds (>= 9.0 mm diameter across the long axis) typically receiving a higher price than smaller seeds. Nash will be a promising alternative to Sierra as a high-yielding large-seeded chickpea cultivar. C1 [Vandemark, G.; Chen, W.; Pfaff, J.; Muehlbauer, F. J.] Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. [Guy, S. O.; Lauver, M.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [McPhee, K.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Vandemark, G (reprint author), Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, 303 Johnson Hall, Pullman, WA 99164 USA. EM george.vandemark@ars.usda.gov FU USDA-ARS; USA Dry Pea and Lentil Council FX Nash was developed with financial support from the USDA-ARS and the USA Dry Pea and Lentil Council. NR 15 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 275 EP 278 DI 10.3198/jpr2014.07.0047crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400001 ER PT J AU Brick, MA Ogg, JB Schwartz, HF Johnson, JJ Judson, F Pastor-Corrales, MA AF Brick, Mark A. Ogg, James B. Schwartz, Howard F. Johnson, Jerry J. Judson, Fred Pastor-Corrales, Marcial A. TI Registration of 'Long's Peak' Pinto Bean SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID DIRECT HARVEST; CULTIVARS AB Methods to harvest the dry edible bean (Phaseolus vulgaris L.) crop have changed dramatically in the past 20 yr to accommodate direct harvest systems. ` Long's Peak' (Reg. No. CV-311, PI 665416; PVP No. 201200395) was released by the Colorado Agricultural Experiment Station to provide dry bean growers with a high-yielding pinto bean cultivar with upright plant architecture for direct harvest systems, and disease resistance. It has medium maturity (95-99 d) and combines resistance to all strains of the common rust pathogen [caused by Uromyces appendiculatus (Pers.) Unger] in the United States and most strains of Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) with excellent seed color and weight. Rust resistance is conditioned by the combination of the Ur-6 and Ur-11 alleles. Resistance to BCMV and BCMNV is conditioned by the allele bc22. Mean seed yield over 11 locations in the Cooperative Dry Bean Nursery in 2011 and 2012 was 3109 and 2840 kg ha(-1) for Long's Peak, respectively, compared with 3022 and 2856 kg ha(-1) for the mean of five test cultivars. Mean seed weight for Long's Peak was 38.3 and 37.4 g 100 seed(-1) compared with the mean 39.8 and 37.7 g 100 seed(-1) among the test cultivars in 2011 and 2012, respectively. Long's Peak will provide producers a pinto cultivar with upright architecture, medium-season maturity, high yield potential, excellent seed quality, and unique resistance to endemic pathogens of the common bean. C1 [Brick, Mark A.; Ogg, James B.; Johnson, Jerry J.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Schwartz, Howard F.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Judson, Fred] Western Colorado Res Stn, Grand Junction, CO 81503 USA. [Pastor-Corrales, Marcial A.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Brick, MA (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM Mark.Brick@Colostate.edu NR 22 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 279 EP 282 DI 10.3198/jpr2015.02.0005crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400002 ER PT J AU Murphy, KM Ullrich, SE Wood, MB Matanguihan, JB Jitkov, VA Guy, SO Chen, XM Brouwer, BO Lyon, SR Jones, SS AF Murphy, Kevin M. Ullrich, Steven E. Wood, Max B. Matanguihan, Janet B. Jitkov, Vadim A. Guy, Stephen O. Chen, Xianming Brouwer, Brook O. Lyon, Steven R. Jones, Stephen S. TI Registration of 'Muir' Spring Feed Barley SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'Muir' (Reg. No. CV-357, PI 674172) is a two-row, spring, hulled feed barley (Hordeum vulgare L.) cultivar developed and evaluated as 07WA-601.6, and released in 2013 by Washington State University (WSU). Muir was derived from the cross 'Baronesse'/'Bob' and selected through single-seed descent from F-2 to F-4 and pedigree breeding methods from F-5 to F-6. Muir was tested in multi-environment trials at 8 to 10 locations per year by the WSU Variety Testing Program from 2011 to 2014. In the low rainfall (<400 mm annual precipitation) testing locations, Muir had a mean grain yield (4787.0 kg ha(-1)) that was higher than those of check cultivars Baronesse, Bob, and Lyon. Muir showed head emergence significantly earlier than Baronesse, Bob, and 'Lenetah' and was 3.3 cm taller than Baronesse and 5.5 cm shorter than 'Champion' across low rainfall zone locations. Muir is resistant to currently prevalent races of the stripe rust pathogen (Puccinia striiformis Westend. f. sp. hordei Erikss.); by comparison, commonly grown cultivars Baronesse, Bob, and Champion are rated as moderately resistant, 'Harrington', Lenetah, and Lyon are rated as moderately susceptible, and 'CDC Copeland' and 'CDC Meredith' are rated as susceptible. Muir was released on the basis of its excellent stripe rust resistance, high grain yield, and agronomic qualities suitable for a feed barley cultivar in low rainfall zones of Washington. C1 [Murphy, Kevin M.; Ullrich, Steven E.; Wood, Max B.; Matanguihan, Janet B.; Jitkov, Vadim A.; Guy, Stephen O.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Chen, Xianming] USDA ARS, Washington, DC 20250 USA. [Brouwer, Brook O.; Lyon, Steven R.; Jones, Stephen S.] Washington State Univ, Northwestern Washington Res & Extens Ctr, Mt Vernon, WA 98273 USA. RP Murphy, KM (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM kmurphy2@wsu.edu FU Washington State Grain Commission; Washington State Agriculture Research Center FX We gratefully acknowledge the assistance of dedicated support personnel in laboratory, greenhouse, and field research during the development of this cultivar, particularly, Kent Evans (USDA-ARS) for disease screening and the many WSU undergraduate students who participated in various aspects of this research. We are especially appreciative of the generous support from the Washington State Grain Commission and the Washington State Agriculture Research Center for providing funding for this research. NR 12 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 283 EP 287 DI 10.3198/jpr2015.01.0001crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400003 ER PT J AU Casler, MD Brink, GE Cherney, JH van Santen, E Humphreys, MW Yamada, T Tamura, K Ellison, NW Opitz, C AF Casler, Michael D. Brink, Geoffrey E. Cherney, Jerome H. van Santen, Edzard Humphreys, Michael W. Yamada, Toshihiko Tamura, Ken-ichi Ellison, Nicholas W. Opitz, Charles TI Registration of 'Hidden Valley' Meadow Fescue SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID MANAGEMENT AB 'Hidden Valley' (Reg. No. CV-100, PI 674472) meadow fescue [Schedonorus pratensis (Huds.) P. Beauv.; syn. Festuca pratensis Huds.; syn. Lolium pratense (Huds.) Darbysh.] is a synthetic population originating from 561 parental genotypes. The original germplasm is of unknown central or northern European origin, but is thought to have become naturalized to the Driftless Area of the Upper Mississippi River Valley over approximately 100 yr. Hidden Valley has forage yield approximately 9% lower than orchardgrass and tall fescue, but neutral detergent fiber digestibility (NDFD) approximately 9% higher than for these two species. Compared to the only other existing meadow fescue cultivar within this region, Hidden Valley has 2% higher forage yield and 1.4% higher NDFD. Hidden Valley is adapted to a wide range of pasture managements and conditions in USDA Hardiness Zones 3 to 5, especially the northern portions of this range. C1 [Casler, Michael D.; Brink, Geoffrey E.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Cherney, Jerome H.] Cornell Univ, Soil & Crop Sci Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [van Santen, Edzard] Auburn Univ, Dept Agron & Soils, Auburn, AL 36849 USA. [Humphreys, Michael W.] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth SY23 3EE, Dyfed, Wales. [Yamada, Toshihiko] Hokkaido Univ, Field Sci Ctr Northern Biosphere, Sapporo, Hokkaido 0600811, Japan. [Tamura, Ken-ichi] Hokkaido Agr Res Ctr, Natl Agr & Food Res Org NARO, Sapporo, Hokkaido 0628555, Japan. [Ellison, Nicholas W.] AgResearch, Grasslands Res Ctr, Palmerston North, New Zealand. [Opitz, Charles] Hidden Valley Farms, Mineral Point, WI USA. RP Casler, MD (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM mdcasler@wisc.edu RI Yamada, Toshihiko/A-5111-2012 OI Yamada, Toshihiko/0000-0002-7845-6556 NR 16 TC 1 Z9 1 U1 2 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 294 EP 298 DI 10.3198/jpr2015.03.0011crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400005 ER PT J AU Jensen, KB Singh, D Bushman, BS Robins, JG AF Jensen, Kevin B. Singh, Devesh Bushman, B. Shaun Robins, Joseph G. TI Registration of 'Arsenal' Meadow Bromegrass SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB The USDA-ARS announces the release of 'Arsenal' meadow bromegrass (Bromus biebersteinii Roem. & Schult. [excluded]) (Reg. No. CV-24, PI 674468) cultivar for use on semiarid rangelands and nonirrigated pastures as a rapid establishing forage grass with early spring growth, good fall nutritional quality, and exceptional winter hardiness. Arsenal is a 39 clone synthetic derived from the same base population as meadow bromegrass cultivar Cache. Arsenal meadow bromegrass was selected for plant vigor, seedling emergence from a deep planting depth, forage and seed yield, and seed mass under dryland environments ranging between 250 and 450 mm of annual precipitation. Molecular marker genotyping demonstrated that Arsenal is genetically different from Cache, 'Fleet', 'Regar', 'Armada', and 'Admiral'. On dryland sites, Arsenal had significantly better seedling establishment and overall stand persistence combined from 2011-2013 than Cache and Regar. Arsenal has been evaluated in New York, Kentucky, Wyoming, and Utah for forage yield and is superior or equal to the cultivar Cache in its ability to produce dry matter yield comparable to or better than smooth bromegrass and meadow bromegrass cultivars under higher precipitation environments in New York and Kentucky. Based on June and September (stockpiled) forage, Arsenal had greater crude protein (17 vs. 7%), in vitro true digestibility (12 vs. 2%), and less neutral detergent fiber (6 vs. 1%) than Cache. Arsenal expands the use of meadow bromegrass from irrigated pastures to nonirrigated pastures and rangelands with >= 330 mm annual precipitation, providing livestock producers with high-yielding nutritious forage that is winter hardy and drought tolerant. C1 [Jensen, Kevin B.; Bushman, B. Shaun; Robins, Joseph G.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Singh, Devesh] Barenbrug USA, Tangent, OR USA. RP Jensen, KB (reprint author), USDA ARS, Forage & Range Res Lab, 695 North 1100 East, Logan, UT 84322 USA. EM kevin.jensen@ars.usda.gov NR 21 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 304 EP 310 DI 10.3198/jpr2015.03.0018crc PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400007 ER PT J AU Sandhu, HS Comstock, JC Gilbert, RA Gordon, VS Korndorfer, P El-Hout, N Arundale, RA AF Sandhu, Hardev S. Comstock, Jack C. Gilbert, Robert A. Gordon, Vanessa S. Korndoerfer, Pedro El-Hout, Nael Arundale, Rebecca A. TI Registration of 'UFCP 78-1013' Sugarcane Cultivar SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID MICROSATELLITE MARKERS; BIOMASS AB Sugarcane (a complex hybrid of Saccharum sp.) cultivar UFCP 78-1013 (Reg. No. CV-162; PI 673049) was developed through the collaborative efforts of the University of Florida (UF) and the USDA-ARS, Canal Point (CP), and released for its potential use in cellulosic ethanol production in Florida. Biomass is the most favorable characteristic of a potential feedstock for cellulosic ethanol production and must be improved in cultivars adapted to Florida conditions should cellulosic ethanol prove feasible for energy production. Averaged across eight location-years of field trials, mean dry biomass yield of UFCP 78-1013 was numerically greater than the reference check, 'L 79-1002'. Plant composition of UFCP 78-1013 is similar to L 79-1002. The parents for UFCP 78-1013 are the sugarcane cultivar CP 68-1067 (female parent) and S. spontaneum line SES 602 (male parent). UFCP 78-1013 has moderate to high resistance against smut (caused by Sporisorium scitamineum) with significantly lower infestation than L 79-1002, under both natural environmental conditions and artificial inoculation. UFCP 78-1013 did not have symptoms of brown rust (caused by Puccinia melanocephala H. & P. Sydow), orange rust (caused by P. kuehnii), mosaic (caused by Sugarcane mosaic virus) or leaf scald [caused by Xanthomonas albilineans (Ashby) Dowson] during field evaluations. UFCP 78-1013 is adapted to marginal or sandy soils of Florida. C1 [Sandhu, Hardev S.] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [Comstock, Jack C.; Gordon, Vanessa S.] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Gilbert, Robert A.] Univ Florida, Gainesville, FL 32611 USA. [Korndoerfer, Pedro] Florida Crystals Corp, South Bay, FL 33493 USA. [El-Hout, Nael] BP Biofuels North Amer LLC, Highlands Cty, FL USA. [Arundale, Rebecca A.] BP Biofuels North Amer LLC, Houston, TX USA. RP Sandhu, HS (reprint author), Univ Florida, Everglades Res & Educ Ctr, 3200 E Palm Beach Rd, Belle Glade, FL 33430 USA. EM hsandhu@ufl.edu OI Gordon, Vanessa S/0000-0001-6490-6985 FU Department of Energy [DE-SC0006995] FX The authors would like to acknowledge the Department of Energy (DE-SC0006995) to support part of this research. We would also like to thank Jairo Sanchez, Richard Bryant, and Calvin Howard from UF to help in data collection and analysis. NR 28 TC 2 Z9 2 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 318 EP 324 DI 10.3198/jpr2014.08.0050crc PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400010 ER PT J AU Ibrahim, AMH Rudd, J Devkota, R Baker, J Sutton, R Simoneaux, B Opena, G Herrington, R Rooney, L Dykes, L Awika, J Nelson, LR Fritz, A Bowden, RL Graybosch, RA Jin, Y Seabourn, BW Chen, XM Kolmer, J Amand, PS Bai, GH Duncan, R AF Ibrahim, Amir M. H. Rudd, Jackie Devkota, Ravindra Baker, Jason Sutton, Russell Simoneaux, Bryan Opena, Geraldine Herrington, Rex Rooney, Lloyd Dykes, Linda Awika, Joseph Nelson, Lloyd R. Fritz, Allan Bowden, Robert L. Graybosch, Robert A. Jin, Yue Seabourn, Bradford W. Chen, Xianming Kolmer, Jim Amand, Paul St. Bai, Guihua Duncan, Robert TI Registration of 'TAM 305' Hard Red Winter Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GERMPLASM; RESISTANT; GREENBUG AB Leaf and stripe rusts (caused by Puccinia triticina Erikss. and P. striiformis Westend. f. sp. tritici Erikss., respectively) are major disease problems in South Texas, Rolling Plains, and the Blacklands area of Texas where hard red winter wheat (HRW; Triticum aestivum L.) is a major crop, and wheat producers in these areas require rust-resistant cultivars. A significant breeding objective in South Texas is to develop HRW cultivars with robust resistance to both rust pathogens. Among these HRW cultivars is 'TAM 305' (Reg. No. CV-1108, PI 674514), which was developed and released by Texas A& M AgriLife Research in 2012. TAM 305 was derived from the cross TX97V3006/TX98V6239. The original cross, designated X00VC230, was made at College Station, TX, in 1999. TAM 305 was released not only for its leaf, stripe, and stem rust (caused by P. graminis Pers.:Pers. f. sp. tritici Erikss. & E. Henn.) resistance but also because of its excellent grain yield in the Blacklands and South Texas. Milling and baking quality characteristics of TAM 305 were rated by the USDA-ARS-Hard Winter Wheat Quality Laboratory in Manhattan, KS. Comparisons to the HRW quality targets showed that TAM 305 has average milling yield, very good wheat and flour protein, minimal mixing time, excellent water absorption and loaf volume, but below average mixing tolerance. C1 [Ibrahim, Amir M. H.; Sutton, Russell; Simoneaux, Bryan; Opena, Geraldine; Herrington, Rex; Rooney, Lloyd; Dykes, Linda; Awika, Joseph; Nelson, Lloyd R.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Rudd, Jackie; Devkota, Ravindra; Baker, Jason] Texas A&M AgriLife Res & Extens, Amarillo, TX 79106 USA. [Fritz, Allan] Kansas State Univ, Manhattan, KS 66506 USA. [Bowden, Robert L.] Kansas State Univ, USDA ARS, Throckmorton Plant Sci Ctr 4007, Manhattan, KS 66506 USA. [Graybosch, Robert A.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Jin, Yue; Kolmer, Jim] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Seabourn, Bradford W.] USDA ARS, Hard Winter Wheat Qual Lab, Manhattan, KS 66502 USA. [Chen, Xianming] Washington State Univ, USDA ARS, Dept Plant Pathol, Pullman, WA 99164 USA. [Amand, Paul St.; Bai, Guihua] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Duncan, Robert] Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada. RP Ibrahim, AMH (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM aibrahim@ag.tamu.edu FU Texas A&M AgriLife Research; Texas Wheat Producers Board FX This work was partly funded with financial support from Texas A&M AgriLife Research and the Texas Wheat Producers Board. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 27 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 325 EP 330 DI 10.3198/jpr2014.08.0054crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400011 ER PT J AU Rudd, JC Devkota, RN Ibrahim, AM Marshall, D Sutton, R Baker, JA Peterson, GL Herrington, R Rooney, LW Nelson, LR Morgan, GD Fritz, AK Erickson, CA Seabourn, BW AF Rudd, Jackie C. Devkota, Ravindra N. Ibrahim, Amir M. Marshall, David Sutton, Russell Baker, Jason A. Peterson, Gary L. Herrington, Rex Rooney, Lloyd W. Nelson, Lloyd R. Morgan, Gaylon D. Fritz, Allan K. Erickson, Charles A. Seabourn, Brad W. TI 'TAM 304' Wheat, Adapted to the Adequate Rainfall or High-Input Irrigated Production System in the Southern Great Plains SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID F-SP TRITICI; REGISTRATION AB 'TAM 304' (Reg. No. CV-1109, PI 655234), a hard red winter wheat (Triticum aestivum L.) cultivar with the experimental designation TX01D3232, was developed and released by Texas A&M AgriLife Research in 2008. TAM 304 is an F-4-derived line from the cross TX92U3060/TX91D6564 made during the winter of 1994-1995. TAM 304 is an awned, medium-early maturing, semidwarf wheat with white glumes. It was released primarily for its excellent grain yield potential particularly in irrigated and adequate rainfall areas across Texas and similar areas in the southern Great Plains, excellent straw strength, resistance to leaf rust (caused by Puccinia triticina Eriks.), and good milling and bread-baking characteristics. Licensed to Scott Seed Company for marketing, TAM 304 is currently one of the most popular hard red winter wheat cultivars adapted to the adequate rainfall or high input irrigated production system in the southern Great Plains. C1 [Rudd, Jackie C.; Devkota, Ravindra N.; Baker, Jason A.; Peterson, Gary L.] Texas A&M AgriLife Res & Extens Ctr, Amarillo, TX 79106 USA. [Ibrahim, Amir M.; Sutton, Russell; Herrington, Rex; Rooney, Lloyd W.; Nelson, Lloyd R.; Morgan, Gaylon D.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Marshall, David] N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. [Fritz, Allan K.] Kansas State Univ, Dept Agron, Throckmorton Plant Sci Ctr 4012, Manhattan, KS 66506 USA. [Erickson, Charles A.] ARS, USDA, Aberdeen, ID 83210 USA. [Seabourn, Brad W.] USDA ARS, Hard Winter Wheat Qual Lab, Manhattan, KS 66502 USA. RP Rudd, JC (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM JCRudd@ag.tamu.edu FU Texas A&M AgriLife Research; Texas Wheat Producers Board FX TAM 304 was developed with funding from Texas A&M AgriLife Research and the Texas Wheat Producers Board. NR 23 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 331 EP 337 DI 10.3198/jpr2015.02.0004crc PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400012 ER PT J AU Liu, L Barnett, MD Griffey, CA Malla, S Brooks, WS Seago, JE Thomason, WE Rucker, E Behl, HD Pitman, RM Dunaway, DW Vaughn, ME Custis, JT Seabourn, B Chen, R Fountain, M Marshall, D Cowger, C Cambron, S Jin, Y Beahm, BR Hardiman, TH Lin, CJ Mennel, DF Mennel, DL AF Liu, L. Barnett, M. D. Griffey, C. A. Malla, S. Brooks, W. S. Seago, J. E. Thomason, W. E. Rucker, E. Behl, H. D. Pitman, R. M. Dunaway, D. W. Vaughn, M. E. Custis, J. T. Seabourn, B. Chen, R. Fountain, M. Marshall, D. Cowger, C. Cambron, S. Jin, Y. Beahm, B. R. Hardiman, T. H. Lin, C. J. Mennel, D. F. Mennel, D. L. TI Registration of 'Vision 45' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'Vision 45' (Reg. No. CV-1110, PI 667642), is a hard red winter (HRW) wheat (Triticum aestivum L.) cultivar that was developed and tested as VA07HRW-45 and released by the Virginia Agricultural Experiment Station in 2012. Vision 45 was derived from the cross 'Provinciale'/'Vision 10' using a modified bulk breeding method. It was tested in replicated yield trials in Virginia (2008-2014) and in the USDA-ARS Uniform Bread Wheat (UBW) Trials (2009-2014). Vision 45 is a widely adapted, high-yielding, awned, semidwarf (unknown Rht gene) HRW wheat with mid-to late-season spike emergence, strong straw strength, and resistance or moderate resistance to diseases prevalent in the mid-Atlantic region. In Virginia, Vision 45 was the highest-yielding hard winter wheat over a 4-yr period, having an average grain yield (5499 kg ha(-1)) that was slightly higher than the soft red winter wheat cultivar Tribute (PI 654422), which was 5181 kg ha(-1). Over 6 yr and 97 environments in the UBW Trials, Vision 45 had an average grain yield of 4924 kg ha(-1), one of the highest-yielding hard wheat cultivars. Vision 45 has good end-use quality on the basis of comparisons with the HRW wheat check cultivar Jagger (PI 593688), for flour yield (70.7 versus 70.3 g 100 g(-1)), flour protein (10.5 versus 10.6 g 100 g(-1)), flour water absorption (60.7 vs. 61.3 g 100 g(-1)), dough mixing tolerance (2.7 versus 2.7), pup-loaf volume (798 versus 773 cm(3)), and crumb grain scores (3.3 versus 2.8). C1 [Liu, L.; Griffey, C. A.; Malla, S.; Brooks, W. S.; Seago, J. E.; Thomason, W. E.; Rucker, E.; Behl, H. D.] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Barnett, M. D.] Limagrain Cereal Seeds LLC, Wichita, KS 67204 USA. [Pitman, R. M.; Dunaway, D. W.; Vaughn, M. E.] Eastern Virginia Agr Res & Ext Ctr, Warsaw, VA 22572 USA. [Custis, J. T.] Eastern Shore Agr Res & Ext Ctr, Painter, VA 23420 USA. [Seabourn, B.; Chen, R.] USDA ARS, Hard Winter Wheat Qual Lab, Manhattan, KS 66502 USA. [Fountain, M.; Marshall, D.; Cowger, C.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Cambron, S.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Jin, Y.] USDA, Cereal Dis Lab, St Paul, MN USA. [Beahm, B. R.] Virginia Fdn Seed Stocks Farm, Mt Holly, VA 22524 USA. [Hardiman, T. H.] Virginia Crop Improvement Assoc, Mechanicsville, VA 23116 USA. [Lin, C. J.; Mennel, D. F.; Mennel, D. L.] Mennel Milling Co, Fostoria, OH 44830 USA. RP Griffey, CA (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM cgriffey@vt.edu FU Virginia Agricultural Experiment Station; Virginia Small Grains Board; Virginia Agricultural Council; Virginia Crop Improvement Association; Mennel Milling Company; USDA-Cooperative State Research, Education, and Extension Service; U.S. Department of Agriculture [2006-55606-16629] FX Vision 45 was developed with financial support from the Virginia Agricultural Experiment Station, the Virginia Small Grains Board, the Virginia Agricultural Council, the Virginia Crop Improvement Association, the Mennel Milling Company, and the USDA-Cooperative State Research, Education, and Extension Service. This material is based on work supported by the U.S. Department of Agriculture under Agreement N. 2006-55606-16629. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 19 TC 1 Z9 1 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 338 EP 344 DI 10.3198/jpr2015.03.0019crc PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400013 ER PT J AU Campbell, BT Jones, MA Greene, JK Jones, DC AF Campbell, B. T. Jones, M. A. Greene, J. K. Jones, D. C. TI Registration of PD 05064, PD 05069, PD 05070, and PD 05071 Germplasm Lines of Cotton SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB PD 05064 (Reg. No. GP-990, PI 674426), PD 05069 (Reg. No. GP-991, PI 674427), PD 05070 (Reg. No. GP-992, PI 674428), and PD 05071 (Reg. No. GP-993, PI 674429) are noncommercial breeding lines of cotton (Gossypium hirsutum L.) jointly released by the USDA-ARS, the Clemson University Experiment Station, and Cotton Incorporated in 2014. PD 05064, PD 05069, PD 05070, and PD 05071 are full-sib breeding lines selected from a cross of PD 94042 and Fibermax 989. PD 05064, PD 05069, PD 05070, and PD 05071 possess broad adaptation and display outstanding fiber quality properties significantly better than several commercial cultivars. Specifically, they possess outstanding fiber strength and length potential, while displaying excellent fiber spinning characteristics superior to commercial cultivars. The four lines display full season maturity and produce lint yields similar to or better than commercial cultivars. PD 05064, PD 05069, PD 05070, and PD 05071 are broadly adapted across the US upland cotton production region. The combination of high yield potential, excellent fiber quality, and superior fiber spinning performance make these four lines valuable genetic resources to cotton breeding programs. C1 [Campbell, B. T.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Jones, M. A.] Clemson Univ, Dep Entomol Soils & Plant Sci, Florence, SC 29506 USA. [Greene, J. K.] Clemson Univ, Blackville, SC 29817 USA. [Jones, D. C.] Cotton Inc, Cary, NC 27513 USA. RP Campbell, BT (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM todd.campbell@ars.usda.gov FU CRIS of the US Department of Agriculture [6657-21000-007-00D]; Cotton Incorporated FX PD 05064, PD 05069, PD 05070, and PD 05071 were developed with financial support from CRIS No. 6657-21000-007-00D of the US Department of Agriculture and Cotton Incorporated. Special thanks to Bobby Fisher and Dan Robinson for technical assistance. Thanks to Chris Delhom for providing AFIS and mini-spinning data. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 8 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 358 EP 362 DI 10.3198/jpr2015.03.0014crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400016 ER PT J AU Campbell, BT Jones, MA Greene, JK Jones, DC AF Campbell, B. T. Jones, M. A. Greene, J. K. Jones, D. C. TI Registration of PD 06001 and PD 06078 Germplasm Lines of Cotton SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB PD 06001 (Reg. No. GP-994, PI 674430) and PD 06078 (Reg. No. GP-995, PI 674431) are noncommercial breeding lines of cotton (Gossypium hirsutum L.) jointly released by the USDA-ARS, the Clemson University Experiment Station, and Cotton Incorporated in 2014. PD 06001 is a breeding line selected from a cross of PD 93007 and 'Sure-Grow 180'. PD 06078 is a breeding line selected from a cross of PD 94042 and 'Sure-Grow 105'. PD 06001 and PD 06078 possess broad adaptation and display outstanding fiber quality properties significantly better than several commercial cultivars. Specifically, PD 06001 and PD 06078 possess outstanding fiber length and strength potential while displaying excellent fiber spinning characteristics superior to commercial cultivars. PD 06001 and PD 06078 display full season maturity and produce lint yields similar to or better than commercial cultivars. PD 06001 and PD 06078 are broadly adapted across the US upland cotton production region. The combination of high yield potential, excellent fiber quality, and superior fiber spinning performance make PD 06001 and PD 06078 valuable genetic resources to cotton breeding programs. C1 [Campbell, B. T.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Jones, M. A.] Clemson Univ, Florence, SC 29506 USA. [Greene, J. K.] Clemson Univ, Blackville, SC 29817 USA. [Jones, D. C.] Cotton Inc, Cary, NC 27513 USA. RP Campbell, BT (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM todd.campbell@ars.usda.gov FU CRIS of the US Department of Agriculture [6657-21000-007-00D]; Cotton Incorporated FX PD 06001 and PD 06078 were developed with financial support from CRIS No. 6657-21000-007-00D of the US Department of Agriculture and Cotton Incorporated. Special thanks to Bobby Fisher and Dan Robinson for technical assistance. Thanks to Chris Delhom for providing AFIS and mini-spinning data. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 8 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 363 EP 366 DI 10.3198/jpr2015.03.0015crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400017 ER PT J AU Landry, EJ Lafferty, JE Coyne, CJ Pan, WL Hu, JG AF Landry, Erik J. Lafferty, Jolene E. Coyne, Clarice J. Pan, William L. Hu, Jinguo TI Registration of Four Winter-Hardy Faba Bean Germplasm Lines for Use in Winter Pulse and Cover Crop Development SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID VICIA-FABA; HARDINESS AB Faba bean (Vicia faba L.) is a versatile crop grown for food, feed, vegetable, or cover crop purposes in many countries. In response to the growing demand for winter annual legumes for cover crop development in the United States, we developed four winter-hardy faba bean germplasm lines, WH-1 (Reg. No. GP-3, PI 674326), WH-2 (Reg. No. GP-4, PI 674327), WH-3 (Reg. No. GP-5, PI 674328), and WH-4 (Reg. No. GP-6, PI 674329) at the USDA-ARS, Western Regional Plant Introduction Station, Pullman, WA, after six consecutive seasons of overwintering selection at three farms in two locations; Pullman and Central Ferry, WA. These four accessions were identified after the initial screening of 175 accessions from the USDA faba bean germplasm during the 2008-2009 and 2009-2010 winter seasons. In the following years, these populations were compared with several winter-type cultivars and breeding lines from Europe. During the final testing season, in 2013-2014, selected and original accessions were compared. The average increase in percentage survival was 84% across the four accessions. Furthermore, the winter hardiness of the selected accessions was comparable to European materials. These four lines are potential reservoirs of useful diversity accessible for the development of a winter annual legume cover crop in the U.S. Pacific Northwest and other regions up to USDA plant hardiness zone 6b. C1 [Landry, Erik J.; Pan, William L.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Lafferty, Jolene E.] Decagon Devices, Pullman, WA 99163 USA. [Coyne, Clarice J.; Hu, Jinguo] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. RP Hu, JG (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM jinguo.hu@ars.usda.gov FU USDA-ARS CRIS [5348-21000-026-00D]; NIFA MultiState Project [W006]; ARCS Foundation FX This research was supported by USDA-ARS CRIS Project 5348-21000-026-00D and NIFA MultiState Project W006 to Jinguo Hu and Scholarship from the ARCS Foundation to Erik Landry. We gratefully acknowledge the assistance with planting and plot maintenance by Sean Vail, Kurt Tetrick, and Wayne Olson. We would also like to thank Lisa Taylor for technical assistance. NR 22 TC 1 Z9 1 U1 2 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 367 EP 370 DI 10.3198/jpr2014.12.0087crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400018 ER PT J AU Carter, TE Todd, SM Gillen, AM AF Carter, T. E., Jr. Todd, S. M. Gillen, A. M. TI Registration of N6001 Soybean Germplasm with Enhanced Yield Derived from Japanese Cultivar Suzuyutaka SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GENETIC DIVERSITY; COEFFICIENT; PARENTAGE; SELECTION; PEDIGREE; LINES; BASE AB The genetic base of US soybean [Glycine max (L.) Merr.] is relatively narrow and derived primarily from Chinese ancestors. Since Japanese lines have been an underutilized resource, their incorporation into US breeding populations may aid soybean improvement. N6001 (Reg No. GP-396, PI 674170) is a conventional soybean germplasm of group VI maturity released by the USDA-ARS and the North Carolina Agricultural Research Service in September 2014. N6001 traces 25% of its pedigree to Japanese cultivar Suzuyutaka and 75% to US cultivar Young. This is the first release of a germplasm derived from Suzuyutaka, which is adapted to North America. N6001 was yield-tested across the southeastern United States in the United Soybean Board Southern Diversity Yield Trial Project and in the USDA Uniform Soybean Tests-Southern States. N6001 yielded 8.9% greater than, and had comparable protein content to, the adapted parent Young (p < 0.05) in the Uniform Tests. N6001 also yielded 8.7% more than Young (p < 0.05) in the Diversity Trials. Over 24 environments, N6001 yielded 98% of elite check cultivar NC-Roy. The improved performance of N6001 over its adapted parent, and near parity with NC-Roy, suggests that yield-enhancing alleles were transferred from Suzuyutaka to N6001. The maintenance of seed protein content with increased yield in N6001 suggests that these genetics may help mitigate the typical negative relation between seed protein content and seed yield. C1 [Carter, T. E., Jr.; Todd, S. M.] USDA ARS, Raleigh, NC 27607 USA. [Gillen, A. M.] USDA ARS, Stoneville, MS 38776 USA. RP Carter, TE (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM Thomas.Carter@ars.usda.gov FU United Soybean Board FX The authors would like to thank the United Soybean Board for funding. The authors would also like to thank the collaborators in the United Soybean Board-sponsored Diversity Trials and USDA Uniform Soybean Tests-Southern States for managing yield trials and sharing data. NR 36 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 376 EP 381 DI 10.3198/jpr2014.09.0057crg PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400020 ER PT J AU Campbell, LG AF Campbell, L. G. TI F1030, F1031, and F1032 Sugarbeet Germplasms Selected from Crosses between L19 and Three Cultivated/Wild Germplasms SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID BETA-VULGARIS; REGISTRATION AB F1030 (Reg. No. GP-288, PI 671774), F1031 (Reg. No. GP-289, PI 671775), and F1032 (Reg. No. GP-290, PI 671776) sugarbeet, Beta vulgaris subsp. vulgaris (L.), germplasm lines were released by the USDA-ARS in 2013. All three lines were selected primarily for sucrose concentration from populations formed by crossing a high-sugar line with three previously released germplasm lines that originated from a cross between a cultivated line and a wild B. vulgaris subsp. maritima (L.) Arcang. accession from Greece. The average sucrose concentration of the three germplasm lines was 15 g kg-1 greater than the average of the three cultivated B. vulgaris subsp. maritima parental lines and 8 g kg(-1) less than the average sucrose concentration of a commercial hybrid. The average root yields of F1030, F1031, and F1032 were 54, 75, and 63% of the root yield of a commercial hybrid. The average recoverable sucrose yield of the three germplasm lines was 906 kg ha(-1) greater than the average sucrose yield of the three cultivated/B. vulgaris subsp. maritima parents. When used as components in the development of advanced breeding populations, F1030, F1031, and F1032 will contribute to a broadening of the genetic diversity within the commercial sugarbeet crop. C1 [Campbell, L. G.] USDA ARS, Sugarbeet & Potato Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. RP Campbell, LG (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM larry.campbell@ars.usda.gov NR 24 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 382 EP 387 DI 10.3198/jpr2015.02.0006crg PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400021 ER PT J AU Eizenga, GC Khush, GS AF Eizenga, Georgia C. Khush, Gurdev S. TI Registration of 24 'IR36' Rice Trisomic Lines SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID TELOTRISOMICS; ORIGIN AB Twenty-four of the 'IR36' rice (Oryza sativa L.) trisomic seed stocks, including 10 primary trisomics lines, Triplo 1, Triplo 3, Triplo 4, Triplo 5, Triplo 7, Triplo 8, Triplo 9, Triplo 10, Triplo 11, and Triplo 12 (Reg. No. GS-9 to GS-18, GSOR 101 to GSOR 110); 7 secondary trisomic lines identified as 2n + 5S.5S, 2n + 7S.7S, 2n + 8S.8S, 2n + 8L.8L, 2n + 11S.11S, 2n + 11L.11L, and 2n + 12S.12S (Reg. No. GS-19 to Reg. No. GS-25, GSOR 111 to GSOR 117), 7 telotrisomic lines identified as 2n + .2L, 2n + .3L, 2n + .7S, 2n + .8S, 2n + .9S, 2n + .10S, and 2n + .12S (Reg. No. GS-26 to Reg. No. GS-32, GSOR 118 to GSOR 124); plus the disomic IR36 (Reg. No. GS-33, GSOR 125) are being made available through the USDA-ARS Genetic Stocks Oryza (GSOR) collection. The lines were imported from the International Rice Research Institute in the Philippines; the trisomic plants were reselected as mature plants in a quarantine greenhouse, and seed from the selected plants was grown in the field for several years at Stuttgart, AR. Segregating trisomic plants were identified based on morphology, and selections were confirmed by meiotic analysis. Data were collected on number of days to heading, plant height, panicle length, flag leaf length and width, number of seeds per panicle, and seed size traits from segregating trisomic plants. C1 [Eizenga, Georgia C.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Khush, Gurdev S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Eizenga, GC (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM georgia.eizenga@ars.usda.gov NR 10 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 398 EP 402 DI 10.3198/jpr2014.11.0079crgs PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400024 ER PT J AU Laudencia-Chingcuanco, D AF Laudencia-Chingcuanco, Debbie TI Registration of Isogenic HMW-GS Ax1 Protein Deficient Lines of an Elite Wheat Cultivar SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GLUTENIN SUBUNIT COMPOSITION; BREAD-MAKING QUALITY; UNITED-STATES; MUTANT; L. AB A series of isogenic wheat (Triticum aestivum L.) lines deficient in high-molecular weight subunit (HMW-GS) proteins encoded on the A-genome of an elite cultivar of bread wheat was developed by USDA-ARS at the Western Regional Research Center in Albany, CA: Ax1-E28H (Reg. No. GS-179, PI 674178); Ax1-F259C (Reg. No. GS-180, PI 674179); Ax1-F261B (Reg. No. GS-181, PI 674180); Ax1-F80B (Reg. No. GS-182, PI 674181); Ax1-F83B (Reg. No. GS-183, PI 674182). This set of A-genome encoded HMW-GS deficient lines was identified from a chemical-and radiation-mutagenized population of hexaploid wheat cultivar Summit by screening M-3 seeds for altered storage protein profiles using one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The mutant lines are distinct from the wildtype progenitor by the absence of the HMW-GS Ax1 protein. Dough derived from greenhouse-and field-grown plants of one mutant exhibited weaker dough but achieved shorter development time compared with the control. Isogenic wheat lines deficient in the individual HMW-GS will allow the determination of the contributions of the different alleles of each locus to dough quality. C1 [Laudencia-Chingcuanco, Debbie] USDA ARS, Western Reg Res Ctr, Genom & Gene Discovery Unit, Albany, CA 94710 USA. RP Laudencia-Chingcuanco, D (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM debbie.laudencia@ars.usda.gov FU USDA-ARS CRIS [5325-21000-019D] FX The excellent technical assistance of the following student interns who helped screen for mutants is greatly appreciated: Lily Lim, Sokchea Pheng, Joshua Speetzen, Huong Phan, and Cindy Chen. The author especially thanks student interns Selina Totanes and Jenny Balayan for their excellent technical assistance in carrying out the mixograph experiments; and Drs. Grace Chen and Sylvia Hua for their critical reading of the manuscript. This work was supported by the USDA-ARS CRIS Project 5325-21000-019D. USDA is an equal opportunity provider and employer. Mention of specific product names in this article does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 13 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 403 EP 406 DI 10.3198/jpr2014.09.0064crgs PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400025 ER PT J AU Belcher, AR Graebner, RC Cuesta-Marcos, A Fisk, S Filichkin, T Smith, KP Blake, VC Hayes, PM AF Belcher, Araby R. Graebner, Ryan C. Cuesta-Marcos, Alfonso Fisk, Scott Filichkin, Tanya Smith, Kevin P. Blake, Victoria C. Hayes, Patrick M. TI Registration of the TCAP FAC-WIN6 Barley Panel for Genomewide Association Studies SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID AUGMENTED DESIGN TYPE-2; WIDE ASSOCIATION; GERMPLASM ARRAYS; SIZE AB Facultative/winter six-row malting barley is a distinct elite germplasm pool and a valuable resource that may prove useful in meeting the challenges of climate change. To preserve its diversity and make it accessible to the research and agricultural communities, the Oregon State University and University of Minnesota barley breeding programs are publicly releasing their winter/facultative six-row malt advanced lines named the TCAP FAC-WIN6 (MP-1, NSL 512632 MAP), which also function as a genomewide association studies (GWAS) panel. The FAC-WIN6 contains 296 lines-180 facultative and 116 winter-selected for disease resistance, malt quality, and general agronomic performance. To date, all lines have data for 6892 single nucleotide polymorphism (SNP) markers and phenotypic data from six experiments (representing 3 yr, eight locations), including traits such as malt quality, disease resistance, nitrogen use efficiency, and winter hardiness. The FAC-WIN6 is one of 24 barley and wheat mapping panels and populations from the USDA-ARS Triticeae Coordinated Agricultural Project (TCAP). As such, all of the TCAP FAC-WIN6 genotypic and phenotypic data can be freely downloaded from the TCAP's online database, T3 (http://triticeaetoolbox.org/barley/). Preliminary GWAS have identified novel loci for wort beta-glucan, low temperature tolerance, and disease resistance. Given these results, the FAC-WIN6 is a singular resource both for future winter six-row barley breeding and for identifying and deploying genes for key barley traits in all backgrounds. C1 [Belcher, Araby R.; Graebner, Ryan C.; Fisk, Scott; Filichkin, Tanya; Hayes, Patrick M.] Oregon State Univ, Plant Breeding & Genet Program, Corvallis, OR 97331 USA. [Cuesta-Marcos, Alfonso] Seminis Vegetable Seeds, Woodland, CA 95695 USA. [Smith, Kevin P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Blake, Victoria C.] USDA ARS, WRRC, Albany, CA 94710 USA. RP Hayes, PM (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM patrick.m.hayes@oregonstate.edu FU American Malting Barley Association; Busch Agricultural Resources, Inc.; Oregon Agricultural Experiment Station, USDA-NIFA [2013-31100-06041]; Oregon Wheat Commission, USDA-NIFA TCAP [2011-68002-30029]; USDA-CSREES-NRI Barley CAP Project [2006-55606-16722] FX The development of the FAC-WIN6 GWAS panel was supported by the American Malting Barley Association, Busch Agricultural Resources, Inc., the Oregon Agricultural Experiment Station, USDA-NIFA Collaborative Project No. 2013-31100-06041, the Oregon Wheat Commission, USDA-NIFA TCAP Project No. 2011-68002-30029, and the USDA-CSREES-NRI Barley CAP Project No. 2006-55606-16722. Special thanks go to Dr. David Hole at University of Utah for generating NUE data; to Dr. Xianming Chen at the USDA-ARS Wheat Genetics, Quality, Physiology, and Disease Research Unit in Pullman, WA, for generating barley stripe rust phenotypic data and race identifications; and to Dr. Cynthia Henson at the USDA-ARS Cereal Crops Research Unit, Madison, WI, for her generous contributions in obtaining the malt quality data. NR 24 TC 0 Z9 0 U1 2 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 411 EP 418 DI 10.3198/jpr2014.12.0083crmp PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400027 ER PT J AU Thompson, AL Smiley, RW Garland-Campbell, K AF Thompson, Alison L. Smiley, Richard W. Garland-Campbell, Kim TI Registration of the LouAu (Louise/IWA8608077) Wheat Recombinant Inbred Line Mapping Population SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID PRATYLENCHUS-NEGLECTUS; LANDRACE WHEAT; THORNEI; RESISTANCE; GROWTH; YIELD AB LouAu (Louise/IWA8608077) (MP-7, NSL 511036) is a wheat (Triticum aestivum L.) recombinant inbred line population developed by the USDA-ARS, with Oregon State and Washington State Universities, from a cross between the soft white spring cultivar Louise and the white facultative Iranian landrace IWA8608077. The population was developed by single seed descent from the F-2 generation to the F-5 generation. The population has 150 F-2:5 recombinant inbred lines and has been used to study the genetics of resistance to root-lesion nematodes (Pratylenchus neglectus and P. thornei), and root architecture. The 26 linkage groups identified include 30 codominant simple sequence repeat markers and 2008 single nucleotide polymorphic markers from the Illumina 9K wheat single nucleotide polymorphism chip. Chi-square analysis shows 21 to 95% of identified polymorphic markers within individual linkage groups were in segregation distortion. The population frequency distributions have a normal distribution for the measured traits P. neglectus resistance, root length, root weight, root lignin content, and plant height. The population frequency distribution has a bimodal distribution for P. thornei resistance, left skewed for lateral root number and right skewed for growth stage. This population has shown potential for mapping resistance to other soilborne pathogens as well as abiotic stresses and will be useful in that endeavor. C1 [Thompson, Alison L.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Smiley, Richard W.] Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. [Garland-Campbell, Kim] Washington State Univ, USDA ARS, Wheat Genet Qual & Physiol, Dis Res Unit, Pullman, WA 99164 USA. RP Garland-Campbell, K (reprint author), 5585 Guilford Rd, Madison, WI 53711 USA. EM kgcamp@wsu.edu FU Washington Grains Commission [3949]; USDA-ARS [5348-21220-003-00D, 5348-22000-013-00]; Washington State University O.A. Vogel grant [8334]; Washington State BIOAg research grant [10A30618915] FX Financial support from the Washington Grains Commission project no. 3949, USDA-ARS (in house) projects: 5348-21220-003-00D and 5348-22000-013-00, the Washington State University O.A. Vogel grant 8334, and the Washington State BIOAg research grant 10A30618915; Vickie Lutes for budget support; Jason Sheedy for help with the parental cross; Kim Kidwell for providing 'Louise' seed; members of the Garland-Campbell laboratory and Steber laboratories for help with the TGA assays; Deven See for providing the 9K SNP consensus map and guidance; Arron Carter for help with the linkage map development; and Scott Hulbert for contributions to the BIOAg research grant. NR 20 TC 1 Z9 1 U1 1 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD SEP PY 2015 VL 9 IS 3 BP 424 EP 429 DI 10.3198/jpr2015.01.0002crmp PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CX1AK UT WOS:000365428400029 ER PT J AU Jabro, JD Iversen, WM AF Jabro, J. D. Iversen, W. M. TI Nondestructive Analysis of Three-dimensional Objects using a Fluid Displacement Method SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID RAY COMPUTED-TOMOGRAPHY; PREFERENTIAL FLOW; SOIL; VISUALIZATION AB Quantification of three-dimensional (3-D) objects has been a real challenge in agricultural, hydrological, and environmental studies. We designed and tested a method that is capable of quantifying 3-D objects using measurements of fluid displacement. The device consists of a stand that supports a movable platform to which the sample is attached. The sample is lowered by measured increments into a vat of fluid. In the original model, as the sample was lowered the displaced fluid overflowed into a container on a scale that sent the weight to a computer. While this worked, the surface tension limited accuracy and it was very slow. To improve the process, the fluid was contained and the fluid level was measured with a linear variable displacement transformer. The displaced fluid volume for each increment and the immersion depth is recorded by a data logger. The surface area of the fluid is reduced as the sample is immersed, so calculations are performed to account for this change. The apparatus was built to facilitate the characterization of three dimensional objects for shape and volume comparisons. It has numerous agricultural and environmental applications. It has been used at our location to quantify the size and length of soil macropores in field soils. It has also been used to numerically evaluate the taper of sugarbeet (Beta vulgaris L.) roots. The apparatus produces good estimates for both aforementioned soil and plant applications. C1 [Jabro, J. D.; Iversen, W. M.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Jabro, JD (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM jay.jabro@ars.usda.gov NR 13 TC 0 Z9 0 U1 4 U2 4 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD SEP-OCT PY 2015 VL 79 IS 5 BP 1289 EP 1292 DI 10.2136/sssaj2015.06.0215 PG 4 WC Soil Science SC Agriculture GA CX0PM UT WOS:000365399100003 ER PT J AU Nauman, TW Thompson, JA Teets, J Dilliplane, T Bell, JW Connolly, SJ Liebermann, HJ Yoast, K AF Nauman, Travis W. Thompson, James A. Teets, Jason Dilliplane, Tim Bell, James W. Connolly, Stephanie J. Liebermann, Henry J. Yoast, Katey TI Pedoecological Modeling to Guide Forest Restoration using Ecological Site Descriptions SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID CHEAT MOUNTAIN SALAMANDER; NORTHERN FLYING SQUIRREL; CENTRAL APPALACHIAN MOUNTAINS; NORTHEASTERN UNITED-STATES; GLAUCOMYS-SABRINUS-FUSCUS; SPRUCE PICEA-RUBENS; RED SPRUCE; WEST-VIRGINIA; PLETHODON-NETTINGI; TRANSITION MODELS AB The U.S. Department of Agriculture (USDA)-Natural Resources Conservation Service (NRCS) uses an ecological site description (ESD) framework to help incorporate interactions between local soil, climate, flora, fauna, and humans into schema for land management decision-making. We demonstrate ESD and digital soil mapping tools to (i) estimate potential O horizon carbon (C) stock accumulation from restoring alternative ecological states in high-elevation forests of the central Appalachian Mountains in West Virginia (WV), USA, and (ii) map areas in alternative ecological states that can be targeted for restoration. This region was extensively disturbed by clear-cut harvests and related fires during the 1880s through 1930s. We combined spodic soil property maps, recently linked to historic red spruce-eastern hemlock (Picea rubens-Tsuga canadensis) forest communities, with current forest inventories to provide guidance for restoration to a historic reference state. This allowed mapping of alternative hardwood states within areas of the spodic shale uplands conifer forest (SCF) ecological site, which is mapped along the regional conifer-hardwood transition of the central Appalachian Mountains. Plots examined in these areas suggest that many of the spruce-hemlock dominated stands in WV converted to a hardwood state by historic disturbance have lost at least 10 cm of O horizon thickness, and possibly much more. Based on this 10 cm estimate, we calculate that at least 3.74 to 6.62 Tg of C were lost from areas above 880 m elevation in WV due to historic disturbance of O horizons, and that much of these stocks and related ecosystem functions could potentially be restored within 100 yr under focused management, but more practical scenarios would likely require closer to 200 yr. C1 [Nauman, Travis W.; Thompson, James A.; Liebermann, Henry J.; Yoast, Katey] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Nauman, Travis W.] USDA NRCS, Natl Soil Survey Ctr, Geospatial Res Unit, Morgantown, WV 26506 USA. [Teets, Jason; Dilliplane, Tim; Bell, James W.; Yoast, Katey] USDA NRCS, MLRA, Soil Survey Off 127, Morgantown, WV 26508 USA. [Connolly, Stephanie J.] US Forest Serv, USDA, Monongahela Natl Forest, Elkins, WV 26241 USA. RP Nauman, TW (reprint author), W Virginia Univ, Div Plant & Soil Sci, POB 6108, Morgantown, WV 26506 USA. EM travis@naumangeospatial.com FU United States' Department of Agriculture Natural Resources Conservation Service FX We acknowledge the large group of scientists who contributed to the field soil description efforts for this project. These individuals came from diverse institutions including USDA-NRCS, USDA Forest Service, West Virginia University, West Virginia State University, and Virginia Polytechnic Institute and State University. We also acknowledge the dedicated fieldwork at forest plots by WVU research associate Aaron Burkholder, and the input and assistance from Shane Jones, MNF Biologist. Portions of this research were supported by the United States' Department of Agriculture Natural Resources Conservation Service. Scientific contribution no. 3255 from the West Virginia Agricultural and Forestry Experiment Station, Morgantown, WV. NR 107 TC 1 Z9 1 U1 3 U2 7 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD SEP-OCT PY 2015 VL 79 IS 5 BP 1406 EP 1419 DI 10.2136/sssaj2015.02.0062 PG 14 WC Soil Science SC Agriculture GA CX0PM UT WOS:000365399100015 ER PT J AU Knoepp, JD Taylor, RS Boring, LR Miniat, CF AF Knoepp, Jennifer D. Taylor, R. Scott Boring, Lindsay R. Miniat, Chelcy F. TI Influence of Forest Disturbance on Stable Nitrogen Isotope Ratios in Soil and Vegetation Profiles SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID LOCUST ROBINIA-PSEUDOACACIA; NATURAL N-15 ABUNDANCE; LONGLEAF PINE; ORGANIC-MATTER; TREE-RINGS; SOUTHERN APPALACHIANS; NUTRIENT ACCUMULATION; FOLIAR DELTA-N-15; CARBON DYNAMICS; ECOSYSTEM AB Soil and plant stable N isotope ratios (delta N-15) are influenced by atmospheric N-2 inputs and processes that regulate organic matter (OM) transformation and N cycling. The resulting delta N-15 patterns may be useful for discerning ecosystem differences in N cycling. We studied two ecosystems, longleaf pine (Pinus palustris Mill.)-wiregrass (Aristida stricta Michx.) (LLP) and Appalachian hardwood (AHW) forests in the US Southeast under different management regimes. In LLP, burning removes OM. In AHW, clearcutting creates large OM pulses of logging residue. Although burning removes OM and clearcutting creates a pulse addition of OM, both management regimes increase soil N availability and N-2-fixing plants. The LLP treatments included burning every 2 yr with N-2 fixers and reference fire exclusion sites without N-2 fixers. The AHW included 25-yr-old clearcut plots with and without N-2 fixers, and uncut reference without N-2 fixers. We hypothesized that: (i) compared with the reference, OM removal (LLP) would enrich delta N-15 values while OM addition (AHW) would deplete delta N-15 in soil and vegetation pools; and (ii) N-2 fixers would mitigate delta N-15 enrichment in LLP response and exacerbate delta N-15 depletion in AHW. We examined total delta N-15 in soil profiles, tree increment cores, and foliage. The LLP soil and vegetation delta N-15 values showed no treatment effect. In AHW, delta N-15 values in clearcut subsurface soils (20-60 cm) were lower than the reference, but N-2 fixers had no effect. Wood delta N-15 differed with treatment; N-2 fixers had no effect. Our data suggest that AHW soil profile delta N-15 patterns may indicate past disturbance; however, wood and foliar delta N-15 response is species specific. Additionally, N-2-fixing plants respond to ecosystem disturbance, but the data suggest that they play little role in soil or plant delta N-15 values. C1 [Knoepp, Jennifer D.; Miniat, Chelcy F.] US Forest Serv, Southern Res Stn, Forest Watershed Sci, Coweeta Hydrol Lab, Otto, NC 28763 USA. [Taylor, R. Scott; Boring, Lindsay R.] JW Jones Ecol Res Ctr Ichauway, Newton, GA 39870 USA. RP Knoepp, JD (reprint author), US Forest Serv, Southern Res Stn, Forest Watershed Sci, Coweeta Hydrol Lab, 3160 Coweeta Lab Rd, Otto, NC 28763 USA. EM jknoepp@fs.fed.us OI Miniat, Chelcy/0000-0002-3266-9783 FU US Forest Service, Southern Research Station; USFS-SRS Challenge Cost-share Grant; NSF [DEB0218001, DEB0823293]; Joseph W. Jones Ecological Research Center; Woodruff Foundation FX This study was supported by the US Forest Service, Southern Research Station, by a USFS-SRS Challenge Cost-share Grant in 2002, by NSF Grants DEB0218001 and DEB0823293 to the Coweeta LTER program at the University of Georgia, and by the Joseph W. Jones Ecological Research Center and the Woodruff Foundation. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the authors and do not necessarily reflect the views of the US Forest Service or the Jones Center. We declare that experiments complied with the current laws of the United States. We declare no conflicts of interest. We thank Patsy Clinton, Kate Sedar, Cindi Brown, and Mary Cobb for their assistance with this research. We are grateful to Dr. Linda Pardo, Dr. Steven Brantley, and anonymous reviewers for their constructive comments on the manuscript. NR 79 TC 1 Z9 1 U1 7 U2 22 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD SEP-OCT PY 2015 VL 79 IS 5 BP 1470 EP 1481 DI 10.2136/sssaj2015.03.0101 PG 12 WC Soil Science SC Agriculture GA CX0PM UT WOS:000365399100020 ER PT J AU Boles, CMW Frankenberger, JR Moriasi, DN AF Boles, C. M. W. Frankenberger, J. R. Moriasi, D. N. TI TILE DRAINAGE SIMULATION IN SWAT2012: PARAMETERIZATION AND EVALUATION IN AN INDIANA WATERSHED SO TRANSACTIONS OF THE ASABE LA English DT Article DE Hydrological modeling; Nitrogen; Phosphorus; SWAT; Tile drainage ID NORTH-EASTERN GERMANY; SUBSURFACE DRAINAGE; NITRATE-NITROGEN; LOWLAND CATCHMENT; ASSESSMENT-TOOL; PHOSPHORUS EXPORT; SURFACE RUNOFF; MODEL; SOIL; TRANSPORT AB Subsurface drainage is an important flow pathway in the poorly drained soils of the Midwestern U.S. and therefore needs to be included in modeling studies. The SWAT2012 tile drainage simulation method, based on the Hooghoudt and Kirkham equations with a drainage coefficient, shows promise for predicting drainage impacts in drained watersheds but has had limited application. In this study, SWAT2012 was implemented in a small agricultural watershed in Indiana using parameters based on knowledge of typical drainage systems and drainage design theory. Monthly Nash-Sutcliffe efficiency (NSE) values for streamflow exceeded 0.70 in all calibration and validation years but were lower for nutrients during some years, with values ranging between 0.14 and 0.88. Simulated tile flow compared well with measured values from field-scale studies in similar locations, ranging from 8.5% to 16.2% of annual precipitation when tile drains were implemented on 50.9% of the watershed. The depth to impermeable layer (DEP_IMP) parameter was found to be the most important calibration parameter, as it also controls seepage through the restrictive layer. Curve number values need to be substantially decreased for tile flow prediction, while two new tile drain parameters that control the ratio of lateral to vertical hydraulic conductivity (LATKSATF) and the static maximum surface roughness (SSTMAXD) likely need to be calibrated, as they could not be assigned values based on real-world properties. This study provides appropriate parameter values for the SWAT2012 tile drainage routine and demonstrates its ability to improve watershed-scale predictions in tile-drained watersheds. C1 [Boles, C. M. W.] LimnoTech, Ann Arbor, MI 48108 USA. [Frankenberger, J. R.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Moriasi, D. N.] USDA ARS, El Reno, OK USA. RP Boles, CMW (reprint author), 501 Avis Dr, Ann Arbor, MI 48108 USA. EM cboles@limno.com FU U.S. Department of Energy [DE-EE0004396] FX Funding for this project was provided by the U.S. Department of Energy (Project DE-EE0004396). Datasets for the St. Joseph River, Indiana, station were provided by the USDA-ARS National Soil Erosion Research Laboratory in West Lafayette, Indiana. NR 59 TC 1 Z9 1 U1 1 U2 7 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD SEP-OCT PY 2015 VL 58 IS 5 BP 1201 EP 1213 PG 13 WC Agricultural Engineering SC Agriculture GA CX0HY UT WOS:000365378700006 ER PT J AU Van Weelden, MB Andersen, DS Trabue, SL Kerr, BJ Rosentrater, KA Pepple, LM AF Van Weelden, M. B. Andersen, D. S. Trabue, S. L. Kerr, B. J. Rosentrater, K. A. Pepple, L. M. TI AN EVALUATION OF THE PHYSICOCHEMICAL AND BIOLOGICAL CHARACTERISTICS OF FOAMING SWINE MANURE SO TRANSACTIONS OF THE ASABE LA English DT Article DE Anaerobic digestion; Deep-pit manure storage; Manure foaming; Methane production; Swine manure ID METHANE; DIGESTION AB Foam accumulation in deep-pit manure storages is an increasing concern for swine producers because of the logistical and safety-related problems it creates. To investigate this phenomenon, samples of swine manure were collected over a 13-month period from 58 swine production facilities in Iowa with varying levels of foam accumulation. Samples were tested for a number of physical, chemical, and biological parameters including pH, total and volatile solids, volatile fatty acid concentration, long-chain free fatty acid concentration, biochemical methane potential, methane production rate, surface tension, foaming capacity, and foam stability. Statistical analysis indicated that manure collected from facilities with foam accumulation produced methane at significantly (p < 0.05) faster rates than non-foaming manures (0.148 +/- 0.004 and 0.049 +/- 0.003 L CH4 L-1 slurry d(-1), respectively) and consequently had significantly (p < 0.05) greater fluxes of biogas moving through the manure. The biochemical methane production assay suggested that manure from foaming pits had less potential to generate methane (123 +/- 9 mL CH4 g(-1) VS) than manure from non-foaming pits (150 +/- 9 mL CH4 g(-1) VS), presumably because more of the methane potential had previously been consumed, as indicated by the higher methane production rates. Short-chain fatty acid concentrations were significantly lower in foaming manures (4200 +/- 570 mg kg(-1)) than non-foaming manures (9470 +/- 730 mg kg(-1)). The methane production rate, biochemical methane potential, and short-chain fatty acid assays suggest enhanced anaerobic digestion efficiency from foaming barns as compared to non-foaming barns. Other assays, such as surface tension and foaming capacity, indicated an accumulation of a surfactant at the manure-air interface of the foam, which may be capturing biogas bubbles generated within the manure. Most importantly, the foam layers exhibited a greatly enhanced ability to stabilize bubbles, which appeared to be correlated to the higher solids concentrations that stabilize the bubbles. C1 [Van Weelden, M. B.; Andersen, D. S.; Rosentrater, K. A.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [Trabue, S. L.; Kerr, B. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Pepple, L. M.] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. RP Andersen, DS (reprint author), Iowa State Univ, 3348 Elings Hall, Ames, IA 50011 USA. EM dsa@iastate.edu FU Iowa Pork Producers Association (Clive, Iowa) FX Funding, in part, was provided by the Iowa Pork Producers Association (Clive, Iowa). NR 24 TC 4 Z9 4 U1 1 U2 2 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD SEP-OCT PY 2015 VL 58 IS 5 BP 1299 EP 1307 PG 9 WC Agricultural Engineering SC Agriculture GA CX0HY UT WOS:000365378700013 ER PT J AU Jung, DH Kim, HJ Choi, GL Ahn, TI Son, JE Sudduth, KA AF Jung, D. H. Kim, H. J. Choi, G. L. Ahn, T. I. Son, J. E. Sudduth, K. A. TI AUTOMATED LETTUCE NUTRIENT SOLUTION MANAGEMENT USING AN ARRAY OF ION-SELECTIVE ELECTRODES SO TRANSACTIONS OF THE ASABE LA English DT Article DE Closed system; Dosing algorithm; Fertilizer; Greenhouse; Hydroponics; Ion sensor; Two-point normalization ID COMPUTER-CONTROLLED SYSTEM; GREENHOUSE CULTIVATION; MEMBRANES; SENSORS; WATER AB Automated sensing and control of macronutrients in hydroponic solutions would allow more efficient management of nutrients for crop growth in closed systems. This article describes the development and evaluation of a computer-controlled nutrient management system with an array of ion-selective electrodes (ISEs) and fertilizer pumps that could effectively manage concentrations of NO3, K, and Ca ions in closed hydroponic systems. A fertilizer dosing algorithm was developed to calculate the volumes of individual nutrient stock solutions to be supplied based on the measurement of present concentrations in a mixing tank. In a five-step spiking test, the system was able to formulate five different concentrations of NO3 and Ca ions comparable to the target concentrations, showing almost 1:1 relationships between the actual and target values. However, actual K concentrations were prepared almost 40% higher than target concentrations due to low K estimates. The use of a two-point normalization method in conjunction with ISEs was effective in minimizing signal drifts resulting from in-line measurement of ion concentrations in the closed system. The automated nutrient management system was used to grow lettuce in a greenhouse with the ebb and flow method After water was automatically added to the mixing tank to maintain the level of water within 10% of the total volume of the tank, the amounts of three different salts, i.e., KNO3, KH2PO4, and Ca(NO3)(2), were variably supplied to the mixing tank based on real-time measurement of concentrations. The three ions were automatically controlled to reach target concentrations of 280, 140, and 70 mg L-1 within errors of -7.7 +/- 28.1, 20.8 +/- 28.5, and -5.6 +/- 8.2 mg L-1 for NO3, K, and Ca ions, respectively. C1 [Jung, D. H.; Kim, H. J.] Seoul Natl Univ, Dept Biosyst Engn & Biomat Sci, Seoul, South Korea. [Choi, G. L.] Natl Inst Hort & Herbal Sci, Protected Hort Res Stn, Busan, South Korea. [Ahn, T. I.; Son, J. E.] Seoul Natl Univ, Dept Plant Sci, Seoul, South Korea. [Sudduth, K. A.] USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. RP Kim, HJ (reprint author), 200-2219,599 Gwanak Ro, Seoul 151921, South Korea. EM kimhj69@snu.ac.kr FU Rural Development Administration, Republic of Korea FX This research was supported in part by the Rural Development Administration, Republic of Korea (2012-2014). NR 17 TC 0 Z9 0 U1 4 U2 11 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD SEP-OCT PY 2015 VL 58 IS 5 BP 1309 EP 1319 PG 11 WC Agricultural Engineering SC Agriculture GA CX0HY UT WOS:000365378700014 ER PT J AU Cai, L Koziel, JA Zhang, S Heber, AJ Cortus, EL Parker, DB Hoff, SJ Sun, G Heathcote, KY Jacobson, LD Akdeniz, N Hetchler, BP Bereznicki, SD Caraway, EA Lim, TT AF Cai, L. Koziel, J. A. Zhang, S. Heber, A. J. Cortus, E. L. Parker, D. B. Hoff, S. J. Sun, G. Heathcote, K. Y. Jacobson, L. D. Akdeniz, N. Hetchler, B. P. Bereznicki, S. D. Caraway, E. A. Lim, T. T. TI ODOR AND ODOROUS CHEMICAL EMISSIONS FROM ANIMAL BUILDINGS: PART 3. CHEMICAL EMISSIONS SO TRANSACTIONS OF THE ASABE LA English DT Article DE Animal feeding operations; Gas chromatography-mass spectrometry; Volatile organic compounds ID SOLID-PHASE MICROEXTRACTION; VOLATILE ORGANIC-COMPOUNDS; MASS SPECTROMETRY-OLFACTOMETRY; FIELD SAMPLING METHOD; FEEDING OPERATIONS; HYDROGEN-SULFIDE; MANURE STORAGE; LIVESTOCK ODORS; NORTH-CAROLINA; GAS EMISSIONS AB The objective of this study was to measure the long-term odor emissions and corresponding concentrations and emissions of 20 odorous volatile organic compounds (VOCs). This study was an add-on study to the National Air Emission Monitoring Study (NAEMS). Odor and odorous gas measurements at four NAEMS sites, including dairy barns in Wisconsin (WI5B) and Indiana (IN5B), a swine finisher barn in Indiana (IN3B), and swine gestation and farrowing barns in Iowa (IA4B), were conducted from November 2007 to May 2009. The odorous gas samples were collected every two weeks using sorbent tubes (samples were collected twice each season of the year, with the exception of spring 2009 when samples were collected three times) and analyzed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). In this article, we summarize the measured gas concentrations and emissions of the 20 target VOCs from each of the four sites. The average total odorous VOC concentrations for the entire sampling period were 276, 96.9, 1413 and 394 mu g dsm(-3) for WI5B, IN5B, IN3B, and IA4B, respectively. For the swine sites, the highest seasonal average total odorous VOC concentrations for each barn were observed during spring (1890 mu g dsm(-3) for IN3B and 458 mu g dsm(-3) for IA4B). For the dairy sites, the highest seasonal average total odorous VOC concentrations were observed in winter at WI5B (446 mu g dsm(-3)) and in summer at IN5B (129 mu g dsm(-3)). The average total emission rates for the 20 odorous VOCs were 290 mg h(-1) AU(-1) (WI5B), 36.0 mg h(-1) AU(-1) (IN5B), 743 mg h(-1) AU(-1) (IN3B), 33.9 mg h(-1) AU(-1) (IA4B swine gestation barns), and 91.7 mg h(-1) AU(-1) (IA4B swine farrowing room). The average seasonal total odorous VOC emission rates were highest during summer at WI5B (805 mg h(-1) AU(-1)), IN5B (121 mg h(-1) AU(-1)), and IN3B (1250 mg h(-1) AU(-1)) and during spring at IA4B (95.8 mg h(-1) AU(-1)). The emissions of specific VOCs varied between seasons, sites, and species. To date, this is the most comprehensive VOC measurement survey of odorous compound emission rates from commercial livestock buildings. C1 [Koziel, J. A.; Hoff, S. J.; Sun, G.; Heathcote, K. Y.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. [Zhang, S.] Fudan Univ, Dept Environm Sci & Engn, Shanghai 200433, Peoples R China. [Heber, A. J.; Bereznicki, S. D.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Cortus, E. L.] S Dakota State Univ, Dept Agr & Biosyst Engn, Brookings, SD USA. [Parker, D. B.] USDA ARS, Bushland, TX USA. [Jacobson, L. D.; Akdeniz, N.; Hetchler, B. P.] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA. [Caraway, E. A.] West Texas A&M Univ, Commercial Core Lab, Canyon, TX USA. [Lim, T. T.] Univ Missouri, Dept Agr Engn, Columbus, MS USA. RP Koziel, JA (reprint author), Iowa State Univ, 4350 Elings Hall, Ames, IA 50011 USA. EM koziel@iastate.edu RI Zhang, Shicheng/H-2399-2011; Koziel, Jacek/B-5483-2014 OI Zhang, Shicheng/0000-0001-9994-1385; Koziel, Jacek/0000-0002-2387-0354 FU USDA-NRI [2005-35112-15336] FX This research was funded by USDA-NRI Grant No. 2005-35112-15336 entitled "Odor emissions and chemical analysis of odorous compounds from animal buildings." NR 53 TC 3 Z9 3 U1 1 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD SEP-OCT PY 2015 VL 58 IS 5 BP 1333 EP 1347 PG 15 WC Agricultural Engineering SC Agriculture GA CX0HY UT WOS:000365378700016 ER PT J AU Zhang, S Koziel, JA Cai, L Hoff, SJ Heathcote, KY Chen, L Jacobson, LD Akdeniz, N Hetchler, BP Parker, DB Caraway, EA Heber, AJ Bereznicki, SD AF Zhang, S. Koziel, J. A. Cai, L. Hoff, S. J. Heathcote, K. Y. Chen, L. Jacobson, L. D. Akdeniz, N. Hetchler, B. P. Parker, D. B. Caraway, E. A. Heber, A. J. Bereznicki, S. D. TI ODOR AND ODOROUS CHEMICAL EMISSIONS FROM ANIMAL BUILDINGS: PART 5. SIMULTANEOUS CHEMICAL AND SENSORY ANALYSIS WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY-OLFACTOMETRY SO TRANSACTIONS OF THE ASABE LA English DT Article DE Air quality; Animal feeding operations; Gas chromatography; Mass spectrometry; Odor; Olfactomehy; Weber-Fechner law ID SOLID-PHASE MICROEXTRACTION; VOLATILE ORGANIC-COMPOUNDS; LIVESTOCK ODORS; THERMAL-DESORPTION; SWINE; OPERATIONS; HEALTH; EFFLUENT AB Simultaneous chemical and sensory analyses using gas chromatography-mass spectrometry-olfactometry (GC-MS-O) for air samples collected at barn exhaust fans were used for quantification and ranking of the odor impacts of target odorous gases. Fifteen target odorous VOCs (odorants) were selected Air samples were collected at dairy barns in Wisconsin and Indiana and at swine barns in Iowa and Indiana over a one-year period The livestock facilities with these barns participated in the National Air Emissions Monitoring Study (NAEMS). Gas concentrations, odor character and intensity, hedonic tone, and odor peak area of the target odorants in air samples were measured simultaneously with GC-MS-O. The four individual odorants emitted from both dairy and swine sites with the largest odor impacts (measured as odor activity value, OAV) were 4-methyl phenol, butanoic acid, 3-methyl butanoic acid, and indole. The total odor (limited to target VOCs and referred to as the measured concentrations, odor intensities, and OAVs) emitted from the swine sites was generally greater than that from the dairy sites. The Weber-Fechner law was used to correlate measured odor intensities with chemical concentrations. Odorants with higher mean OAV followed the Weber-Fechner law much better than odorants with lower mean OAV. The correlations between odor intensities and chemical concentrations were much better for the swine sites (typically p < 0.05 and R-2 = 0.16 to 0.51) than for the dairy sites (typically p > 0.05 and R-2 < 0.15). Linking specific gases to odor could assist in the development and evaluation of odor mitigation technologies for solving livestock odor nuisance problems. C1 [Zhang, S.; Chen, L.] Fudan Univ, Dept Environm Sci & Engn, Shanghai 200433, Peoples R China. [Zhang, S.; Koziel, J. A.; Cai, L.; Hoff, S. J.; Heathcote, K. Y.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [Cai, L.] DuPont Crop Protect, Newark, DE USA. [Jacobson, L. D.; Akdeniz, N.; Hetchler, B. P.] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA. [Parker, D. B.] USDA ARS, Bushland, TX USA. [Parker, D. B.] West Texas A&M Univ, Canyon, TX USA. [Caraway, E. A.] West Texas A&M Univ, Div Agr, Canyon, TX USA. [Heber, A. J.; Bereznicki, S. D.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN USA. RP Koziel, JA (reprint author), Iowa State Univ, 4350 Elings Hall, Ames, IA 50011 USA. EM koziel@iastate.edu RI Zhang, Shicheng/H-2399-2011; Koziel, Jacek/B-5483-2014 OI Zhang, Shicheng/0000-0001-9994-1385; Koziel, Jacek/0000-0002-2387-0354 FU USDA-NRI [2005-35112-15336] FX This research was funded by USDA-NRI Grant No. 2005-35112-15336 entitled "Odor Emissions and Chemical Analysis of Odorous Compounds from Animal Buildings." NR 35 TC 2 Z9 2 U1 3 U2 11 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD SEP-OCT PY 2015 VL 58 IS 5 BP 1349 EP 1359 PG 11 WC Agricultural Engineering SC Agriculture GA CX0HY UT WOS:000365378700017 ER PT J AU Warburton, ML Tang, JD Windham, GL Hawkins, LK Murray, SC Xu, WW Boykin, D Perkins, A Williams, WP AF Warburton, Marilyn L. Tang, Juliet D. Windham, Gary L. Hawkins, Leigh K. Murray, Seth C. Xu, Wenwei Boykin, Debbie Perkins, Andy Williams, W. Paul TI Genome-Wide Association Mapping of Aspergillus flavus and Aflatoxin Accumulation Resistance in Maize SO CROP SCIENCE LA English DT Article ID GERMPLASM LINE; KERNEL INFECTION; EAR ROT; REGISTRATION; POPULATION; IDENTIFICATION; MYCOTOXINS; MP313E; CORN AB Contamination of maize (Zea mays L.) with aflatoxin, produced by the fungus Aspergillus flavus Link, has severe health and economic consequences. Efforts to reduce aflatoxin accumulation in maize have focused on identifying and selecting germplasm with natural host resistance factors, and several maize lines with significantly reduced aflatoxin accumulation have been identified. Past linkage mapping studies have identified quantitative trait loci (QTL) that consistently reduce aflatoxin levels in maize. In addition, an association mapping panel of 300 maize inbred lines was previously created specifically for the dissection of aflatoxin accumulation resistance. Here we report the results of a genome-wide association study (GWAS) using this panel of testcrossed maize hybrids. Each of the inbred parents of the testcrossed hybrids was genotyped by sequencing to generate 261,184 robust single nucleotide polymorphisms (SNPs), and the entire panel was phenotyped for aflatoxin accumulation following inoculation with A. flavus in multilocation, replicated field trials. Results uncovered 107 SNPs associated with aflatoxin accumulation in one or more environments in the association panel at a probability level between 9.78 x 10(-6) and 2.87 x 10(-10). Eight SNP trait associations were found with a false discovery rate (FDR) of less than 10% (p < 3.83 x 10(-7)). These SNPs occur within the sequence of three uncharacterized genes. Variants in 25 other genomic regions showing high association values over more than one environment are also presented. These genomic regions are undergoing validation studies and will be of use to dissect the resistance to aflatoxin accumulation and improve this trait. C1 [Warburton, Marilyn L.; Tang, Juliet D.; Windham, Gary L.; Hawkins, Leigh K.; Williams, W. Paul] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Murray, Seth C.] Texas A&M Univ, Dep Soil & Crop Sci, College Stn, TX USA. [Xu, Wenwei] Texas A&M Univ, Dep Soil & Crop Sci, Lubbock, TX USA. [Boykin, Debbie] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. [Perkins, Andy] Mississippi State Univ, Dept Comp Sci & Engn, Mississippi State, MS USA. RP Warburton, ML (reprint author), USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. EM marilyn.warburton@ars.usda.gov FU USDA; joint USDA-USAID Feed the Future initiative FX The authors wish to thank Jack Haynes for his excellent technical assistance, the Cornell Institute for Genomic Diversity for their help in generating and analyzing the GBS data, and Drs. W. Suwarno and I. Barrero for their critical review of the manuscript. This work was supported by the USDA and the joint USDA-USAID Feed the Future initiative. This paper is a joint contribution of USDA-ARS and the Mississippi Agricultural and Forestry Experiment Station and is published as Journal No. J-12668 of the Miss. Agric. and Forestry Exp. Stn. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. NR 48 TC 4 Z9 4 U1 5 U2 24 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 1857 EP 1867 DI 10.2135/cropsci2014.06.0424 PG 11 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400001 ER PT J AU Bowman, BC Chen, J Zhang, J Wheeler, J Wang, Y Zhao, W Nayak, S Heslot, N Bockelman, H Bonman, JM AF Bowman, B. C. Chen, J. Zhang, J. Wheeler, J. Wang, Y. Zhao, W. Nayak, S. Heslot, N. Bockelman, H. Bonman, J. M. TI Evaluating Grain Yield in Spring Wheat with Canopy Spectral Reflectance SO CROP SCIENCE LA English DT Article ID IN-SEASON PREDICTION; WINTER-WHEAT; VEGETATION INDEXES; INDIRECT SELECTION; GREAT-PLAINS; WATER STATUS; PLANT; ASSOCIATION; IMPROVEMENT; DROUGHT AB Worldwide, improving grain yield is the most important target for wheat (Triticum aestivum L.) breeders. Fast, cost-effective, and nondestructive phenotyping methods for important traits are needed to increase the efficiency of cultivar development. The present study tested canopy spectral reflectance (CSR) as a potential high-throughput method for assessing wheat grain yield in a diverse set of 540 spring-habit accessions from the USDA-ARS National Small Grains Collection. Plots were grown under irrigated (IR) and terminal drought (DR) treatments over two growing seasons, and CSR was measured at several growth stages in each year. The CSR indices related to canopy water and N status, biomass, and photosynthetic area were evaluated for their relation to grain yield. The CSR indices were significantly correlated with yield at every growth stage, with anthesis and grain filling being the most useful for predicting grain yield in IR and DR environments. Single CSR indices selected up to 57% of the highest 25% yielding lines in DR conditions and the grain yield of accessions selected using CSR was 20% greater than randomly selected genotypes. Canopy spectral reflectance also identified up to 86% of the highest 10% yielding accessions. Canopy spectral reflectance may be valuable as a high-throughput means of selecting for yield in large trials of genetically diverse wheat genotypes. C1 [Bowman, B. C.; Chen, J.; Zhang, J.; Wheeler, J.; Wang, Y.; Zhao, W.; Nayak, S.] Univ Idaho, Dept Plant Soil & Entomol Sci, Aberdeen, ID 83210 USA. [Heslot, N.] Cornell Univ, Dept Genet & Plant Breeding, Ithaca, NY 14853 USA. [Bockelman, H.; Bonman, J. M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. RP Chen, J (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, 1693 S 2700 W, Aberdeen, ID 83210 USA. EM jchen@uidaho.edu FU USDA National Institute of Food and Agriculture Triticeae [CAP 2011-68002-30029] FX This study was supported by the USDA National Institute of Food and Agriculture Triticeae-CAP 2011-68002-30029. We would like to thank Dr. Luther Talbert, Dr. Mark Sorrells, and Dr. Jorge Dubcovsky for their input in editing this manuscript. NR 49 TC 2 Z9 2 U1 4 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 1881 EP 1890 DI 10.2135/cropsci2014.08.0533 PG 10 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400003 ER PT J AU Dahleen, LS Bregitzer, P Mornhinweg, D Klos, KE AF Dahleen, Lynn S. Bregitzer, Phil Mornhinweg, Do Klos, Kathy Esvelt TI Genetic Diversity for Russian Wheat Aphid Resistance as Determined by Genome-Wide Association Mapping and Inheritance in Progeny SO CROP SCIENCE LA English DT Article ID GERMPLASM LINES RESISTANT; BARLEY GERMPLASM; SPRING BARLEY; REGISTRATION; STARS-9301B; SOFTWARE; MAPS AB Russian wheat aphid (RWA) [Diuraphis noxia (Mordvilko)] is an increasing problem on barley (Hordeum vulgare L.) throughout the world. Genetic resistance has been identified and used to create barley germplasm and cultivars adapted to the United States. Several mapping studies have been conducted to identify loci associated with resistance, but questions remain, particularly regarding the extent of genetic diversity among resistance sources and improved lines and cultivars. Association analysis of resistant (R) and moderately resistant (MR), unadapted accessions held in the USDA-ARS National Small Grains Germplasm Collection, adapted germplasm lines derived from these accessions, and susceptible (S) elite cultivars was conducted using 4509 single nucleotide polymorphisms (SNPs) to identify markers linked to resistance. Resistance was associated with loci on chromosomes 1H, 2H, 3H, 5H, and 7H at p-values < 0.0001, and the major 1H and 3H loci identified in previous tests were confirmed. However, the genetics of RWA resistance was shown to be complex, and it is likely that loci involved in resistance in some lines were not identified. Nevertheless, this analysis identified diversity among R lines and showed that most of the released RWA-resistant cultivars had similar genotypes at loci associated with resistance. This analysis also identified R lines that were genetically dissimilar to the released cultivars at these loci. This information can guide parental selection for future breeding efforts designed to capture and deploy greater diversity for RWA resistance among new barley cultivars. C1 [Dahleen, Lynn S.] USDA ARS, NCSL, Fargo, ND 58102 USA. [Mornhinweg, Do] USDA ARS, Stillwater, OK 74075 USA. [Bregitzer, Phil; Klos, Kathy Esvelt] USDA ARS, Natl Small Grains Germplasm Res Facil, Aberdeen, ID 83210 USA. RP Bregitzer, P (reprint author), USDA ARS, Natl Small Grains Germplasm Res Facil, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM phil.bregitzer@ars.usda.gov FU USDA-ARS CRIS [5442-21000-036, 5366-21000-28, 6217-21000-008] FX This research was funded by USDA-ARS CRIS projects 5442-21000-036, 5366-21000-28, and 6217-21000-008. Mention of trade names or commercial products in this publication is solely to provide specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity employer. NR 26 TC 0 Z9 0 U1 4 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 1925 EP 1933 DI 10.2135/cropsci2014.09.0634 PG 9 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400007 ER PT J AU Hagerty, CH Cuesta-Marcos, A Cregan, PB Song, Q McClean, P Noffsinger, S Myers, JR AF Hagerty, C. H. Cuesta-Marcos, A. Cregan, P. B. Song, Q. McClean, P. Noffsinger, S. Myers, J. R. TI Mapping Fusarium solani and Aphanomyces euteiches Root Rot Resistance and Root Architecture Quantitative Trait Loci in Common Bean SO CROP SCIENCE LA English DT Article ID PHASEOLUS-VULGARIS; IDENTIFICATION; MARKER; GENOME; MAPS AB Root rot diseases of bean (Phaseolus vulgaris L.) are a constraint to dry and snap bean production. We developed the RR 138 RI mapping population from the cross of OSU5446, a susceptible line that meets current snap bean processing industry standards, and RR 6950, a root rot resistant dry bean with small brown seeds. We evaluated the RR 138 RI population beginning in the F-6 generation for resistance to Fusarium solani f. sp. phaseoli (Burk.) root rot in Oregon and Aphanomyces euteiches (Drechsler) root rot in Wisconsin. The population was evaluated for a set of root architecture traits at the Oregon location. Fusarium solani root rot resistance was evaluated in three seasons, whereas A. euteiches resistance was evaluated in two seasons. For each disease, RR 6950 was resistant and OSU 5446 was susceptible. The recombinant inbred (RI) population was normally distributed for reaction to both diseases. We assembled a high-density linkage map using 1689 single-nucleotide polymorphisms (SNPs) from an Illumina 6000-SNP BARCbean6K_3 Beadchip. The map spanned 1196 cM over 11 linkage groups at a density of one SNP per 1.4 cM. Three quantitative trait loci (QTL) associated with A. euteiches were identified, each accounting for 5 to 15% of the total genetic variation, and two QTL associated with F. solani resistance accounted for 9 and 22% of the total genetic variation. A QTL for taproot diameter (TD) and one QTL for basal root angle were identified. The QTL for resistance to the two diseases mapped to different genome locations indicating a different genetic control. C1 [Hagerty, C. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Myers, J. R.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Cuesta-Marcos, A.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Cregan, P. B.; Song, Q.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [McClean, P.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Noffsinger, S.] Seneca Foods Corp, Dayton, WA 99328 USA. RP Myers, JR (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. EM james.myers@oregonstate.edu FU Seneca Foods Corporation; USDA-NIFA Bean CAP project [2009-01929] FX Author contributions: C.H. Hagerty contributed to design and conducted experiments and drafted the manuscript. A. Cuesta-Marcos assisted C.H. Hagerty with linkage map construction and QTL analysis. P. Cregan and Q. Song developed the SNP platform used in the study and conducted allele calling of SNPs. P. McClean contributed data from the Stampede. Redhawk RI population, with physical location of SNPs and gene annotation from the Phaseolus genome sequence. S. Noffsinger conducted Aphanomyces screening trials in Wisconsin. J.R. Myers conceived of the project, developed the recombinant inbred population, supervised the research, and assisted in writing the publication. We are grateful for the assistance of Randy Hopson for his expertise in managing the field plots and to Shawna Zimmerman and Kara Young who helped with the root rot rating in Oregon. Joel Davis provided excellent support in the laboratory. A grant from Seneca Foods Corporation provided support for research activities and a graduate research assistantship for CHH. We also gratefully acknowledge the support of the USDA-NIFA Bean CAP project (project number 2009-01929) in making the SNP characterization of the mapping population possible by providing access to the Illumina chip. NR 36 TC 5 Z9 5 U1 1 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 1969 EP 1977 DI 10.2135/cropsci2014.11.0805 PG 9 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400011 ER PT J AU Li, XH Alarcon-Zuniga, B Kang, JM Tahir, MHN Jiang, QZ Wei, YL Reyno, R Robins, JG Brummer, EC AF Li, Xuehui Alarcon-Zuniga, Baldomero Kang, Junmei Tahir, M. Hammad Nadeem Jiang, Qingzhen Wei, Yanling Reyno, Rafael Robins, Joseph G. Brummer, E. Charles TI Mapping Fall Dormancy and Winter Injury in Tetraploid Alfalfa SO CROP SCIENCE LA English DT Article ID TUBEROSUM SUBSP TUBEROSUM; MEDICAGO-SATIVA L.; LINKAGE MAP; FREEZING TOLERANCE; COLD-ACCLIMATION; GENE-EXPRESSION; LOW-TEMPERATURE; HARDINESS; GROWTH; QTL AB Alfalfa (Medicago sativa L.) is a widely planted perennial forage crop. Fall dormancy is generally negatively correlated with winter injury in alfalfa. To understand the genetic basis of the two traits, we identified quantitative trait loci (QTL) controlling autumn growth and winter injury using a tetraploid alfalfa F-1 population. In total, 601 marker alleles were scored from 78 restriction fragment length polymorphism (RFLP), 123 simple-sequence repeat (SSR), and 48 single nucleotide polymorphism (SNP) markers. Linkage maps were constructed for each parent separately. Both maps contained eight linkage groups (LGs), with a length of 898 cM for WISFAL-6 and 845 cM for ABI408. Using interval mapping, we identified 15 QTL from an across-environment analysis and 71 QTL within individual environments for autumn plant height; winter injury; and autumn shoot, crown, and root biomass across four Iowa environments. Of the 71 QTL, 42 were identified at 18 chromosomal locations that were identified in multiple environments for the same trait. Possible pleiotropic QTL that contributed to dry weight of shoot, crown, and taproot were found, which partially explained the observed genetic correlations between those traits. However, few QTL were related to both autumn plant height and winter injury, supporting the observation of no genetic correlation between the two traits in this study. These results indicated that the two traits could be manipulated independently and, possibly, efficiently improved using marker-assisted selection. Because most QTL identified in this study were mapped to intervals of at least 10 cM, validation and localization in additional populations is needed to facilitate application of marker-assisted selection. C1 [Li, Xuehui] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Alarcon-Zuniga, Baldomero] Univ Autonoma Chapingo, Dept Anim Sci, Texcoco 56230, Mexico. [Kang, Junmei] Chinese Acad Agr Sci, Dept Turf & Forage Sci, Beijing 100193, Peoples R China. [Tahir, M. Hammad Nadeem] Univ Agr Faisalabad, Plant Breeding & Genet, Faisalabad, Pakistan. [Jiang, Qingzhen] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK 73401 USA. [Reyno, Rafael] INIA, Natl Inst Agr Res, Tacuarembo, Uruguay. [Robins, Joseph G.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Wei, Yanling; Brummer, E. Charles] Univ Calif Davis, Plant Breeding Ctr, Davis, CA 95616 USA. [Wei, Yanling; Brummer, E. Charles] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Li, XH (reprint author), N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. EM Xuehui.Li@ndsu.edu; ecbrummer@ucdavis.edu FU USDA SDA-IFAFS [00-52100-9611] FX This research was funded in part by USDA SDA-IFAFS Grant No. 00-52100-9611 to ECB. We thank Mark Smith for field plot assistance. NR 57 TC 2 Z9 2 U1 6 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 1995 EP 2011 DI 10.2135/cropsci2014.12.0834 PG 17 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400013 ER PT J AU Varella, AC Weaver, DK Sherman, JD Blake, NK Heo, HY Kalous, JR Chao, S Hofland, ML Martin, JM Kephart, KD Talbert, LE AF Varella, A. C. Weaver, D. K. Sherman, J. D. Blake, N. K. Heo, H. Y. Kalous, J. R. Chao, S. Hofland, M. L. Martin, J. M. Kephart, K. D. Talbert, L. E. TI Association Analysis of Stem Solidness and Wheat Stem Sawfly Resistance in a Panel of North American Spring Wheat Germplasm SO CROP SCIENCE LA English DT Article ID CEPHUS-CINCTUS NORTON; TRITICUM-AESTIVUM L.; GENOMIC SELECTION; MARKERS; HYMENOPTERA; CEPHIDAE; IMPACT; IDENTIFICATION; PARASITISM; DAMAGE AB The expanding geographical range of wheat stem sawfly (WSS) damage, coupled with the limited number of effective control measures, calls for a need to better characterize and explore the genetic variability for resistance in wheat (Triticum aestivum L.) germplasm from North America. An association-mapping analysis for stem solidness and wheat stem sawfly resistance was conducted using a set of 244 elite spring wheat lines from 10 North American breeding programs. Three previously identified quantitative trait loci (QTL) for resistance were confirmed, including QTL for stem solidness, heading date, and stem cutting. Three new QTL on chromosomes 2A, 3A, and 5B were identified to be associated with larval mortality and a QTL associated with early stem solidness was identified on chromosome 5D. Genetic variation for late stem solidness is mainly determined by the solid-stem QTL on chromosome 3B, while variation for early solidness is affected by chromosomal regions on 1B, 3B, and 5D. Potential sources of a novel resistance mechanism causing larval mortality were identified among the panel lines. Favorable alleles for stem solidness and other resistance traits are available within North American wheat germplasm, but only Montana and South Dakota breeding programs appear to have favorable alleles for all QTLs detected in this study. Improvements in wheat stem sawfly management, for both historically impacted areas and for regions first experiencing damage, may be obtained using alleles that already exist in elite germplasm. C1 [Varella, A. C.; Sherman, J. D.; Blake, N. K.; Heo, H. Y.; Kalous, J. R.; Martin, J. M.; Talbert, L. E.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Weaver, D. K.; Hofland, M. L.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Chao, S.] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Kephart, K. D.] Southern Agr Res Ctr, Huntley, MT 59037 USA. RP Talbert, LE (reprint author), Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. EM usslt@montana.edu FU Montana Wheat and Barley Committee; USDA National Institute of Food and Agriculture [2011-68002-30029, 2013-67013-21106] FX This research was supported by grants from the Montana Wheat and Barley Committee and by USDA National Institute of Food and Agriculture awards 2011-68002-30029 and 2013-67013-21106. Lines were provided to the SW-AM panel by Karl Glover (South Dakota State University), Jianli Chen (University of Idaho), Jorge Dubcovsky (University of California-Davis), Mike Pumphrey (Washington State University), James Anderson (University of Minnesota), Pierre Hucl (University of Saskatchewan), Curtis Pozniak (University of Saskatchewan), Luther Talbert (Montana State University), Dean Spaner (University of Alberta), Gavin Humphries (Agriculture and Agri-Food Canada), Ron DePauw (Agriculture and Agri-Food Canada), Ron Knox (Agriculture and Agri-Food Canada), and the International Maize and Wheat Improvement Center (CIMMYT). NR 32 TC 4 Z9 4 U1 3 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2046 EP 2055 DI 10.2135/cropsci2014.12.0852 PG 10 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400016 ER PT J AU Hoyos-Villegas, V Mkwaila, W Cregan, PB Kelly, JD AF Hoyos-Villegas, Valerio Mkwaila, Wezi Cregan, Perry B. Kelly, James D. TI Quantitative Trait Loci Analysis of White Mold Avoidance in Pinto Bean SO CROP SCIENCE LA English DT Article ID BUNSI-DERIVED RESISTANCE; PHASEOLUS-VULGARIS L.; PHYSIOLOGICAL RESISTANCE; AGRONOMIC TRAITS; QTL ANALYSIS; YIELD; IDENTIFICATION; REGISTRATION; INHERITANCE; DISEASE AB White mold is a major disease of common bean (Phaseolus vulgaris L.) grown in temperate production areas. The objective of this study was to use single nucleotide polymorphism (SNP) markers from the BARCBean6K_3 BeadChip to identify quantitative trait loci (QTL) associated with traits related to white mold resistance in common bean. A recombinant inbred line (RIL) population from a cross of disease-tolerant pinto line AN-37 and disease-susceptible line P02630 was evaluated in Michigan for 4 yr under white mold pressure. Traits evaluated included disease incidence, the numbers of days to flower and to maturity, canopy height, lodging, seed yield, and 100-seed weight. A linkage map of the RIL population spanning 1499 cM was constructed using 447 SNP markers. The map covered all 11 bean chromosomes with an average distance of 3.6 cM between markers. A total of 13 QTL for agronomic and disease traits were consistently identified in different years. A major QTL WM3.1AN associated with white mold avoidance was validated on chromosome Pv03. The QTL is associated with disease avoidance traits such as canopy porosity, plant height, stay green stem trait, and maturity. Quantitative trait loci for maturity and canopy height also mapped to the same genomic region on Pv03. Finding strong associations between maturity, lodging, canopy height, and disease incidence offers alternative strategies to improve levels of white mold avoidance over greenhouse screening. The validation of the WM3.1(AN, AP630) QTL for white mold avoidance should provide bean breeders with the opportunity to introgress avoidance traits into their germplasm. C1 [Hoyos-Villegas, Valerio; Mkwaila, Wezi; Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Cregan, Perry B.] USDA ARS, Beltsville Agr Res Ctr, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Kelly, JD (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM kellyj@msu.edu FU USDA-ARS National Sclerotinia Initiative [58-5442-3-269]; Michigan State University AgBioResearch; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2009-01929] FX The research was supported by the USDA-ARS National Sclerotinia Initiative Grant No. 58-5442-3-269 Identification of QTL for White Mold Resistance in Pinto Bean, by Michigan State University AgBioResearch and by the Agriculture and Food Research Initiative Competitive Grant no. 2009-01929 from the USDA National Institute of Food and Agriculture. The authors acknowledge E. Wright for assistance with field trials and C. Quigley for the SNP calling. NR 52 TC 3 Z9 3 U1 0 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2116 EP 2129 DI 10.2135/cropsci2015.02.0106 PG 14 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400023 ER PT J AU Casler, MD Cherney, JH Brummer, EC Dien, BS AF Casler, Michael D. Cherney, Jerome H. Brummer, E. Charles Dien, Bruce S. TI Designing Selection Criteria for Use of Reed Canarygrass as a Bioenergy Feedstock SO CROP SCIENCE LA English DT Article ID PERENNIAL GRASSES; BIOMASS YIELD; PHALARIS-ARUNDINACEA; HARVEST MANAGEMENT; NUTRITIONAL-VALUE; FORAGE CROPS; ENERGY CROPS; QUALITY; SWITCHGRASS; PERSISTENCE AB Reed canarygrass (Phalaris arundinacea L.) is a perennial C3 grass with a circumglobal distribution in the Northern Hemisphere and adaptation to a wide range of environmental conditions. This species is currently under development as a bioenergy feedstock in North America and Europe. Thus, the objectives of this study were to (i) quantify genetic variation for biomass quality traits of reed canarygrass cultivars and accessions, (ii) determine the relationships for biomass yield with reed canarygrass quality traits, and (iii) identify favorable and attainable breeding objectives for biomass yield and quality traits of reed canarygrass. Eighty cultivars or accessions were grown at five field locations and evaluated for 26 biomass quality traits in 2006 through 2008. Ignoring biomass quality traits in favor of the singular breeding objective of high biomass yield would be likely to result in a few favorable correlated responses including low N, low ash, low Cl, low Fe, and high enzymatic xylan conversion efficiency. These changes would not be sufficiently large to transform reed canarygrass into a more favorable bioenergy feedstock within a few generations of breeding. To do so will require a more focused effort with a relatively small number of traits, for example, high biomass yield, low ash, and high gross calorific value (GCV) for a thermochemical conversion platform or high biomass yield, low lignin, and high glucan and xylan conversion efficiencies for a fermentation platform. C1 [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Cherney, Jerome H.] Cornell Univ, Sch Integrat Plant Sci, Soil & Crop Sci Sect, Ithaca, NY 14853 USA. [Brummer, E. Charles] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Dien, Bruce S.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Casler, MD (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM mdcasler@wisc.edu OI Dien, Bruce/0000-0003-3863-6664 NR 41 TC 1 Z9 1 U1 1 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2130 EP 2137 DI 10.2135/cropsci2015.03.0142 PG 8 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400024 ER PT J AU Cichy, KA Porch, TG Beaver, JS Cregan, P Fourie, D Glahn, RP Grusak, MA Kamfwa, K Katuuramu, DN McClean, P Mndolwa, E Nchimbi-Msolla, S Pastor-Corrales, MA Miklas, PN AF Cichy, Karen A. Porch, Timothy G. Beaver, James S. Cregan, Perry Fourie, Deidre Glahn, Raymond P. Grusak, Michael A. Kamfwa, Kelvin Katuuramu, Dennis N. McClean, Phil Mndolwa, Eninka Nchimbi-Msolla, Susan Pastor-Corrales, Marcial A. Miklas, Phillip N. TI A Phaseolus vulgaris Diversity Panel for Andean Bean Improvement SO CROP SCIENCE LA English DT Article ID GENETIC DIVERSITY; POPULATION-STRUCTURE; ELECTROPHORETIC VARIABILITY; DISSEMINATION PATHWAYS; CORE COLLECTION; SEQUENCE DATA; COMMON BEANS; L.; REGISTRATION; DOMESTICATION AB Dry beans (Phaseolus vulgaris L.) of the Andean gene pool, including red mottled, kidney, cranberry, and yellow seed types are important in Africa and the Americas. Andean dry bean breeding gains have lagged behind those of Mesoamerican beans. This difference may result from a narrower genetic base in the Andean gene pool and reduced breeding efforts. The objective of this research was to establish, genotype, and phenotype a panel of bean germplasm to be used for Andean dry bean breeding. An Andean diversity panel (ADP) was assembled, consisting of 396 accessions and including important cultivars, breeding lines, and landraces that originate mostly from Africa, the Caribbean, and North and South America. The panel was genotyped using the Illumina BARCBean6K_3 SNP BeadChip. The population contained two sub-groups: Andean and Mesoamerican bean germplasm. The ADP was comprised of 349 Andean, 21 Mesoamerican, and 26 Andean-Meso-american admixed accessions. Most admixed lines came from Africa (12 accessions) and the Caribbean (five accessions). Association mapping was conducted for determinacy. Significant single-nucleotide polymorphism (SNP) trait associations were found on chromosome Pv01, with the most significant SNP marker being 3.1 kb from the Terminal Flower 1 PvTFL1y gene. The ADP was evaluated for numerous traits in field trials in the United States and Africa. Variability was found for resistance to rust, angular leaf spot and common bacterial blight diseases; tolerance to low soil fertility; cooking time; and other traits that can be used to improve Andean bean germplasm for Africa and the Americas. C1 [Cichy, Karen A.] USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48823 USA. [Porch, Timothy G.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR USA. [Beaver, James S.] Univ Puerto Rico, Dept Crop & Agroenvironm Sci, Mayaguez, PR USA. [Cregan, Perry; Pastor-Corrales, Marcial A.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD USA. [Fourie, Deidre] Grain Crops Inst, Agr Res Council, Potchefstroom, South Africa. [Glahn, Raymond P.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Grusak, Michael A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med,Dept Pediat, Houston, TX 77030 USA. [Cichy, Karen A.; Kamfwa, Kelvin; Katuuramu, Dennis N.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [McClean, Phil] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Mndolwa, Eninka] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Nchimbi-Msolla, Susan] Sokoine Univ Agr, Dept Crop Sci & Prod, Morogoro, Tanzania. [Miklas, Phillip N.] USDA ARS, Grain Legume Genet Physiol Res Unit, Prosser, WA 99350 USA. RP Cichy, KA (reprint author), USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48823 USA. EM karen.cichy@ars.usda.gov FU Norman Borlaug Commemorative Research Initiative (US Agency for International Development); USDA Agricultural Research Service [58-6250-0-008] FX This work was supported, in part, by funding from the Norman Borlaug Commemorative Research Initiative (US Agency for International Development), and by the USDA Agricultural Research Service through Cooperative Agreement Number 58-6250-0-008 (MAG). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. We also thank Jose Luis Claros for DNA extraction. NR 61 TC 10 Z9 10 U1 2 U2 12 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2149 EP 2160 DI 10.2135/cropsci2014.09.0653 PG 12 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400026 ER PT J AU Harris, DK Kendrick, MD King, ZR Pedley, KF Walker, DR Cregan, PB Buck, JW Phillips, DV Li, ZL Boerma, HR AF Harris, Donna K. Kendrick, Mandy D. King, Zachary R. Pedley, Kerry F. Walker, David R. Cregan, Perry B. Buck, James W. Phillips, Daniel V. Li, Zenglu Boerma, H. Roger TI Identification of Unique Genetic Sources of Soybean Rust Resistance from the USDA Soybean Germplasm Collection SO CROP SCIENCE LA English DT Article ID SOUTHEASTERN UNITED-STATES; BULKED SEGREGANT ANALYSIS; PHAKOPSORA-PACHYRHIZI; 1ST REPORT; CONFERS RESISTANCE; FLORIDA BEGGARWEED; GOLDENGATE ASSAY; CAUSAL AGENT; RAPID METHOD; LINKAGE MAP AB Soybean [Glycine max (L.) Merr.] rust is caused by the fungal pathogen Phakopsora pachyrhizi. Six rust resistance loci (Rpp1, 2, 3, 4, 5, and 6) have been reported. Crosses were made between 75 resistant plant introductions (PIs) and a susceptible elite line or cultivar. Bulked segregant analysis (BSA) was used to determine if the PI resistance genes mapped to a previously identified locus or to an unreported locus. Fifty-two PIs had resistance genes that mapped to the Rpp3 region on chromosome 6 of the soybean genome. A set of P. pachyrhizi isolates was used to further characterize the resistance of these PIs. Forty-two of the PIs exhibited the same reaction profiles as either PI 462312 (Rpp3) or 'Hyuuga' (Rpp3 and Rpp5) to the panel of isolates. The fine mapping of Rpp1, Rpp3, and Rpp4 and the availability of the SoySNP50K Infinium Chip data on the USDA Soybean Germplasm Collection made it possible to use BSA and isolate data on these PIs to determine in retrospect how effective haplotype analysis would be in narrowing down the PIs to those likely to have a unique source of resistance. Thirty-seven of the 52 PIs (71%) mapping to the Rpp3 region had a haplotype identical to that of PI 462312. A combination of these analyses could prove useful in more rapidly narrowing down resistant PIs to those likely to carry a unique resistance gene. C1 [Harris, Donna K.; King, Zachary R.; Li, Zenglu; Boerma, H. Roger] Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. [Kendrick, Mandy D.; Pedley, Kerry F.] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Walker, David R.] USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Buck, James W.; Phillips, Daniel V.] Univ Georgia, Dept Plant Pathol, Griffin, GA 30223 USA. RP Harris, DK (reprint author), Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. EM dkharri@uga.edu FU Georgia Agricultural Experiment Stations; USDA-ARS; United Soybean Board; T.W. Edminster Postdoctoral Fellowship FX The authors would like to express their appreciation to the following individuals: Amy Ruck (USDA-ARS, Fort Detrick, MD) for her assistance with the characterization of the plant introductions to the panel of Phakopsora pachyrhizi isolates at the USDA-ARS Foreign Disease-Weed Science Research Unit at Fort Detrick, MD; Dr. Dechun Wang and Dr. Zixaing Wen from the Soybean Genetics Lab at Michigan State University for the Infinium HD assay services provided; and Billy Mills, David Spradlin, Richard Miller, Dale Wood, Gina Rowan, and Earl Baxter (Univ. of Georgia) for their assistance with the planting and management of this research at Attapulgus, GA, and in the greenhouse at Griffin, GA. This research was partially funded by the Georgia Agricultural Experiment Stations, the USDA-ARS and grants from the United Soybean Board. M.D. Kendrick was supported, in part, by the T.W. Edminster Postdoctoral Fellowship awarded to K.F. Pedley. NR 53 TC 5 Z9 5 U1 1 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2161 EP 2176 DI 10.2135/cropsci2014.09.0671 PG 16 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400027 ER PT J AU Kolmer, JA AF Kolmer, J. A. TI Leaf Rust Resistance in Wheat Line RL6062 is an Allele at the Lr3 Locus SO CROP SCIENCE LA English DT Article ID F-SP TRITICI; COMMON WHEAT; SYSTEM; GENE AB RL6062, a 'Thatcher' backcross line of wheat (Triticum aestivum L.), has resistance to leaf rust (caused by Puccinia triticina Erikss.) derived from a line of common wheat from Iran. RL6062 has an intermediate infection type (IT) of small to moderate uredinia surrounded by distinct chlorosis. RL6062 was crossed with Thatcher and the F-2 seedlings and F-3 families segregated for a single leaf rust resistance gene. Bulk segregant analysis of homozygous resistant and homozygous susceptible F-3 families using diversity arrays technology (DArT) markers indicated that the leaf rust resistance was on chromosome 6BL. RL6062 was crossed with Thatcher lines with genes Lr9, Lr3a, Lr3b, and Lr3c that are also located on chromosome 6BL. The F-2 seedlings of RL6010-Lr9/RL6062 segregated for IT that ranged from immune response to high IT with large uredinia that lacked necrosis or chlorosis. The F-3 families derived from F-2 seedlings with high IT were homozygous susceptible or segregated for intermediate IT and high IT, confirming that resistance in RL6062 was not allelic to Lr9. The F-2 seedlings of RL6002-Lr3a/RL6062 segregated for IT that varied from flecks to moderately large size uredinia that lacked chlorosis or necrosis. The F-3 families derived from F-2 seedlings that had intermediate to high IT segregated for low IT of fleck to intermediate IT, and none of the seedlings had high IT. The lack of segregation for susceptible plants in the F-3 families indicated that the leaf rust resistance in RL6062 is an allele at the Lr3 locus. Alleles at the Lr3 locus had intermediate to recessive expression. C1 [Kolmer, J. A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM jim.kolmer@ars.usda.gov NR 13 TC 0 Z9 0 U1 1 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2186 EP 2190 DI 10.2135/cropsci2015.01.0031 PG 5 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400029 ER PT J AU Beavers, AW Goggi, AS Reddy, MB Lauter, AM Scott, MP AF Beavers, Alyssa W. Goggi, A. Susana Reddy, Manju B. Lauter, Adrienne Moran Scott, M. Paul TI Recurrent Selection to Alter Grain Phytic Acid Concentration and Iron Bioavailability SO CROP SCIENCE LA English DT Article ID CELL-CULTURE MODEL; INORGANIC PHOSPHORUS; ASCORBIC-ACID; IN-VITRO; MAIZE; PHYTATE; ABSORPTION; SEEDS; FERRITIN; PROTEIN AB Iron is an important micronutrient and Fe deficiency is a global health concern. Phytic acid inhibits Fe absorption and cannot be digested by monogastric livestock or humans. High phytate concentration in staple crops may be one of the contributing factors for the high incidence of anemia in developing countries because of its inhibiting effect on Fe absorption. In seeds, it serves as the main storage compound for P. Low phytic acid mutants (lpa) in maize (Zea mays L.) have improved Fe bioavailability, but they have poor germination. Our objective was to develop both low phytic acid (LPA) and high phytic acid (HPA) maize populations using recurrent selection and to compare seed quality and Fe bioavailability among the HPA and LPA populations and lpa mutant lines. Three cycles of selection were performed in two broad-based synthetic populations, BS11 and BS31. The resulting HPA and LPA populations were significantly different in phytic acid concentration in the BS11-derived populations (P < 0.05) but not in the BSS31-derived populations (P > 0.05). The BS11LPA maize population had improved seed germination (13-16%; P < 0.05), and Fe bioavailability was not statistically different (P > 0.05) than the lpa mutant inbred lines. We conclude that recurrent selection for phytic acid levels may be a viable approach for improving Fe bioavailability of grain while maintaining seed quality. C1 [Beavers, Alyssa W.] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Goggi, A. Susana] Iowa State Univ Sci & Technol, Dept Agron, Ames, IA 50011 USA. [Goggi, A. Susana] Seed Sci Ctr, Ames, IA USA. [Reddy, Manju B.] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA USA. [Lauter, Adrienne Moran; Scott, M. Paul] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50010 USA. RP Scott, MP (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50010 USA. EM paul.scott@ars.usda.gov FU Iowa State University Office of Biotechnology; Hatch Funds [IOW04114]; USDA-NIFA-OREI program FX The authors wish to acknowledge Dr. Kendall Lamkey for the gift of the lpa1-1 mutant lines and the BS11 and BS31 populations used in this study and Mr. Jay Hoch for technical assistance. AWB was supported by a fellowship from the Iowa State University Office of Biotechnology. ASG participation was possible by Hatch Funds IOW04114. Part of this work was made possible by a grant from the USDA-NIFA-OREI program to MPS. We express our gratitude to the Seed Science Center and Iowa State Seed Laboratory for providing assistance and supplies for the seed quality determinations. Product names are necessary to report factually on the available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may be suitable. The USDA is an equal opportunity provider and employer. NR 46 TC 0 Z9 0 U1 2 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2244 EP 2251 DI 10.2135/cropsci2014.12.0807 PG 8 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400035 ER PT J AU Hardegree, SP Walters, CT Boehm, AR Olsoy, PJ Clark, PE Pierson, FB AF Hardegree, Stuart P. Walters, Christina T. Boehm, Alex R. Olsoy, Peter J. Clark, Patrick E. Pierson, Frederick B. TI Hydrothermal Germination Models: Comparison of Two Data-Fitting Approaches with Probit Optimization SO CROP SCIENCE LA English DT Article ID SEED DORMANCY LOSS; BROMUS-TECTORUM L; S-AND-H; TIME MODEL; CONSTANT TEMPERATURES; GRAIN LEGUMES; THRESHOLD MODELS; FIELD; EMERGENCE; GRASSES AB Probit models for estimating hydrothermal germination rate yield model parameters that have been associated with specific physiological processes. The desirability of linking germination response to seed physiology must be weighed against expectations of model fit and the relative accuracy of predicted germination response. Computationally efficient empirical models have been proposed that do not require a priori assumptions about model shape parameters, but the accuracy of these models has not been compared to the more common probit-optimization procedure. Thirteen seedlots, representing six native perennial rangeland grasses and an invasive annual weed, were germinated over the constant temperature range of 3 to 36 degrees C and water potential range of 0 to -2.5 MPa. Hydrothermal germination models were generated using probit optimization, optimized regression, and statistical gridding. These models were evaluated for the pattern and magnitude of residual model error and the relative magnitude of predictive errors under field-simulated temperature and moisture conditions. Residual model errors in predictions of germination rate were greatest for the probit optimization procedure. Statistical gridding and optimized regression produced lower predictive model error, but the latter procedure could not resolve germination response for slower-germinating seed populations. The more computationally efficient and accurate regression and statistical-gridding procedures may be desirable for identifying germination strategies and syndromes that are based on predicted response to simulated conditions of field temperature and moisture. C1 [Hardegree, Stuart P.; Boehm, Alex R.; Clark, Patrick E.; Pierson, Frederick B.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Walters, Christina T.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Olsoy, Peter J.] Boise State Univ, Boise Ctr Aerosp Lab, Boise, ID 83725 USA. RP Hardegree, SP (reprint author), USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. EM stuart.hardegree@ars.usda.gov NR 45 TC 2 Z9 3 U1 1 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2276 EP 2290 DI 10.2135/cropsci2014.10.0703 PG 15 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400039 ER PT J AU Brenner, DM Dekker, J Niemi, J Pfiffner, L AF Brenner, David M. Dekker, Jack Niemi, Jarad Pfiffner, Lisa TI Medical Oxygen Concentrators for Releasing Seed Dormancy SO CROP SCIENCE LA English DT Article ID GASEOUS ENVIRONMENT; COVERING STRUCTURES; RICE SEED; GERMINATION; TEMPERATURE; STORAGE; VIGOR; LIGHT AB A new method is demonstrated for using concentrated oxygen (O-2 gas) to release seed dormancy. Concentrated O-2 gas in air is known to release seed dormancy in some seeds, including some foxtail (Setaria) species. New medical equipment makes O-2 gas easier to work with than before, so laboratories working with dormant seeds can now use concentrated O-2 gas as a seed treatment on a production basis. Use of medical O-2 gas concentrators is simpler and safer than using O-2 gas supplied by pressurized gas cylinders. Suitable medical O-2 gas concentrators in new or used condition are readily available, operate on standard electrical current, and deliver O-2 gas with low-pressure tubes and fittings. Resealable plastic bags are inflated with concentrated O-2 gas and then sealed as seed treatment chambers. This use of concentrated O-2 gas is confirmed to significantly increase the germination of dormant seeds of giant foxtail (Setaria faberi Herrm) and plains bristlegrass (S. macrostachya Kunth). C1 [Brenner, David M.] Iowa State Univ, Dept Agron, Plant Introduct Stn, Ames, IA 50011 USA. [Niemi, Jarad] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. USDA ARS, Plant Introduct Res Unit, Ames, IA 50011 USA. [Pfiffner, Lisa] North Cent Reg Plant Introduct Stn, Ames, IA 50011 USA. RP Brenner, DM (reprint author), Iowa State Univ, Dept Agron, Plant Introduct Stn, G212 Agronomy Hall, Ames, IA 50011 USA. EM dbrenner@iastate.edu FU USDA National Institute of Food and Agriculture [Hatch P IOW01018]; State of Iowa FX Choice Medical, Ames, IA, provided the O2 gas concentrator and also verified its functioning before the experiment. Samuel N. Flomo prepared the seed samples. Robert P. Ewing and Candice A. C. Gardner made helpful suggestions for the manuscript. Research was conducted at the North Central Regional Plant Introduction Station in Ames, IA, and supported by the USDA National Institute of Food and Agriculture (Hatch P IOW01018) and by State of Iowa funds. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement. NR 18 TC 0 Z9 0 U1 3 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2291 EP 2293 DI 10.2135/cropsci2014.11.0783 PG 3 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400040 ER PT J AU Peel, MD Ransom, CV Mott, IW Waldron, BL AF Peel, Michael D. Ransom, Corey V. Mott, Ivan W. Waldron, Blair L. TI Natural Glyphosate Tolerance in Sweetvetch Hedysarum boreale SO CROP SCIENCE LA English DT Article ID HERBICIDE GLYPHOSATE; DOSE-RESPONSE; RESISTANCE; BIOSYNTHESIS; MECHANISMS; HORSEWEED; BIOTYPES; ALFALFA AB Sweetvetch (Hedysarum boreale Nutt.) is purported to tolerate glyphosate [N-(phosphonomethyl) glycine]. Eight rates of glyphosate were tested for their effect on biomass (BM) and survival of seedlings and mature plants. Rates were 0, 210, 420, 841, 1681, 3363, 6725, and 13,450 g a.e. ha(-1). Tall fescue (Festuca arundinaceae Schreb.) was included as a susceptible check. Doses required to cause 50% reduction (GR(50)) in sweetvetch seedling BM was 5025 and 1636 g a. e. ha(-1) at first and second harvests, respectively. Tall fescue seedling BM GR(50) values were 373 and 512 g a. e. ha(-1) at first and second harvests, respectively. Seedling mortality in sweetvetch was not sufficient to estimate an LD50; however, in tall fescue the seedling LD50 was 987 g a. e. ha(-1). Four weeks after spring field treatments, sweetvetch and tall fescue BM GR(50) values were 3467 and 549 g a. e. ha(-1), respectively. Thirteen months later BM GR(50) estimates were 11,595 and 886 g a. e. ha(-1) for sweetvetch and tall fescue, respectively. Fall-applied glyphosate did not reduce BM in the sweetvetch sufficient to estimate a GR(50). Tall fescue estimated BM GR(50) was 413 g a. e. ha(-1). At the three lowest treatments BM of sweetvetch mature plants was unaffected. Survival of mature sweetvetch plants was >= 70% precluding estimates of LD50 values. The LD50's for tall fescue spring and fall treatments was 751 and 541 g a. e. ha(-1), respectively. Based BM GR 15 values sweetvetch will withstand a glyphosate rate of 670 g a. e. ha(-1) with no mortality or negative effect on subsequent harvests. C1 [Peel, Michael D.; Mott, Ivan W.; Waldron, Blair L.] Utah State Univ, USDA ARS, Forage & Range Res Unit, Logan, UT 84322 USA. [Ransom, Corey V.] Utah State Univ, Dept Plant Soils & Climate, Logan, UT 84322 USA. RP Peel, MD (reprint author), Utah State Univ, USDA ARS, Forage & Range Res Unit, Logan, UT 84322 USA. EM mike.peel@ars.usda.gov NR 33 TC 0 Z9 0 U1 1 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2368 EP 2376 DI 10.2135/cropsci2014.08.0571 PG 9 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400048 ER PT J AU Castillo, MS Sollenberger, LE Mullenix, MK Blount, AR Williams, MJ Mackowiak, CL AF Castillo, Miguel S. Sollenberger, Lynn E. Mullenix, M. Kimberly Blount, Ann R. Williams, Mary J. Mackowiak, Cheryl L. TI Grazing Management Affects Establishment Performance of Rhizoma Peanut Strip Planted into Bahiagrass Pasture SO CROP SCIENCE LA English DT Article ID SEASON GRASS PASTURE; WARM-SEASON; LEGUME; FLORIGRAZE AB Establishing rhizoma peanut (Arachis glabrata Benth.; RP) in strips into existing bahiagrass (Paspalum notatum Flugge) pastures has potential to increase forage nutritive value, decrease N fertilizer use, and improve N cycling. Grazing the grass-legume mixture in the year after planting may be possible, but appropriate grazing management strategies have not been defined. The objectives were to determine (i) the effect of year-after-planting (Y2) grazing management on 'Florigraze' RP performance when strip planted in bahiagrass swards, and (ii) the interaction of Y2 defoliation strategies with those imposed in the year of planting (Y1). Treatments were the factorial combinations of four Y1 defoliation strategies (no defoliation, hay production, simulated continuous stocking, and rotational stocking every 28 d) and three Y2 grazing frequencies (simulated continuous, 28 d, and 42 d to a 15-cm bahiagrass stubble height). Grazing pastures in Y2 reduced RP contribution relative to that at the end of Y1. Canopy cover and frequency decreased from 30 to 10% and 80 to 50%, respectively, due to Y2 grazing for both Y1 no defoliation and hay production treatments. Cover remained below 10% in Y2 for both Y1 simulated continuous and rotational stocking treatments. Spread of RP into adjacent bahiagrass was greater in Y2 plots that in Y1 were not defoliated or were used for hay production (similar to 27 cm) than grazed (similar to 0 cm) plots. Results indicate that grazing during Y2 negatively affects RP establishment regardless of grazing frequency or RP cover at the beginning of Y2. Cattle preference for RP resulted in overgrazing; thus, if grazing occurs during RP establishment, target endpoints should be based on RP strips not the surrounding bahiagrass. C1 [Castillo, Miguel S.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Sollenberger, Lynn E.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Mullenix, M. Kimberly] Auburn Univ, Dept Anim Sci, Auburn, AL 36849 USA. [Blount, Ann R.; Mackowiak, Cheryl L.] North Florida Res & Educ Ctr, Marianna, FL 32446 USA. [Williams, Mary J.] USDA, NRCS, Gainesville, FL 32614 USA. RP Castillo, MS (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM mscastil@ncsu.edu NR 17 TC 0 Z9 0 U1 4 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD SEP-OCT PY 2015 VL 55 IS 5 BP 2384 EP 2389 DI 10.2135/cropsci2015.02.0090 PG 6 WC Agronomy SC Agriculture GA CW5GT UT WOS:000365024400050 ER PT J AU Bertini, C AF Bertini, Catherine TI y Invisible Women SO DAEDALUS LA English DT Article AB Women are ubiquitous and critical to the nutritional well-being of their families, yet they are often invisible to policy-makers, public officials, community leaders, and researchers. Effecting significant decreases in the number of hungry poor people, as well as the improvement of nutritional and economic outcomes, requires policy in addition to operational and research priorities that are directed at the needs of women and girls. C1 [Bertini, Catherine] Amer Acad, New York, NY 10010 USA. [Bertini, Catherine] Syracuse Univ, Publ Adm & Int Affairs, Maxwell Sch Citizenship & Publ Affairs, Syracuse, NY 13244 USA. [Bertini, Catherine] USDA, Food & Consumer Serv, New York, NY USA. [Bertini, Catherine] United Nations World Food Programme, Geneva, Switzerland. RP Bertini, C (reprint author), Amer Acad, New York, NY 10010 USA. NR 14 TC 0 Z9 0 U1 0 U2 0 PU MIT PRESS PI CAMBRIDGE PA ONE ROGERS ST, CAMBRIDGE, MA 02142-1209 USA SN 0011-5266 EI 1548-6192 J9 DAEDALUS-US JI Daedalus PD FAL PY 2015 VL 144 IS 4 BP 24 EP 30 DI 10.1162/DAED_a_00351 PG 7 WC Humanities, Multidisciplinary; Social Sciences, Interdisciplinary SC Arts & Humanities - Other Topics; Social Sciences - Other Topics GA CW8YW UT WOS:000365286400003 ER PT J AU Roberts, AJ Petersen, MK Funston, RN AF Roberts, A. J. Petersen, M. K. Funston, R. N. TI BEEF SPECIES SYMPOSIUM: Can we build the cowherd by increasing longevity of females? SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT Beef Species Symposium Joint Annual Meeting CY JUL 20-24, 2014 CL Kansas City, MO SP Journal Anim Sci, Amer Soc Anim Sci DE beef cattle; cow longevity; reproduction; stayability ID LIFETIME PRODUCTIVITY; GENETIC-PARAMETERS; CROSSBRED COWS; HEREFORD COWS; CATTLE; STAYABILITY; HETEROSIS; HEIFERS; SYSTEMS; ANGUS AB Increasing longevity of beef cows by decreasing the proportion culled due to reproductive failure provides an efficient process to rebuild a cow herd and can reduce number of replacements needed to sustain a constant herd size. Rate of reproductive failure varies due to cow age, where failure in cows 2 to 4 yr of age is often greater than in cows 5 to 7 yr of age. In addition, BW of cow and calf at weaning increase as cows advance from 2 to 5 yr of age. The cumulative effect of increasing retention of young cows is improved production efficiency through decreased replacement rate and changing age structure of the herd resulting in a greater proportion of cows at maximal production potential for calf BW at weaning and cow BW at time of culling. Calculations from cow age-specific culling and BW data from commercial and research herds indicated that reducing replacement rate from 18% to 14% resulted in a 23% increase in calf BW weaned and a 2% increase in cull cow BW per pregnant replacement heifer going into the herd. Although improving longevity increases production efficiency, genetic advancement in sustained reproductive function is challenging, as it is the sequential culmination of the annual repetition of numerous discrete physiological processes, each ending in a qualitative response. Successful completion of one process is prerequisite to evaluating subsequent processes. These physiological processes are subject to nutritional threshold requirements that may vary due to genetic potential for other production traits such as milk, growth, and mature size resulting in genetic-by-nutrition interactions. This is in contrast to most traits for which EPD exist, where genetic-by-environment interactions are not considered to be significant. Extensive research concerning impact of limited nutrition on reproduction has led to recommendations that heifers and cows be fed to a threshold BW or BCS to ensure reproductive success; a process that masks nutritional interactions that might otherwise result in reproductive failure. This management approach minimizes selection for animals capable of sustained reproductive function under limited nutritional environments. Rearing and managing cows under nutritionally limited environments may lead to adaptations that result in relatively high levels of reproductive success under lower input levels. Such adaptation may improve chances for longer retention in their offspring in nutrient-limited environments. C1 [Roberts, A. J.; Petersen, M. K.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Funston, R. N.] Univ Nebraska, West Cent Res & Extens Ctr, North Platte, NE 69101 USA. EM andy.roberts@ars.usda.gov FU Journal of Animal Science; American Society of Animal Science FX on a presentation at the Beef Species Symposium titled "Making more, but using less: The future of the U.S. beef industry with a reduced cowherd and the challenge to feed the US and the world" at the Joint Annual Meeting, July 20-24, 2014, Kansas City, Missouri, with publication sponsored by the Journal of Animal Science and the American Society of Animal Science. NR 39 TC 2 Z9 2 U1 1 U2 11 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD SEP PY 2015 VL 93 IS 9 BP 4235 EP 4243 DI 10.2527/jas.2014-8811 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CW7IR UT WOS:000365172300003 PM 26440322 ER PT J AU Johnson, JS Fernandez, MVS Seibert, JT Ross, JW Lucy, MC Safranski, TJ Elsasser, TH Kahl, S Rhoads, RP Baumgard, LH AF Johnson, J. S. Fernandez, M. V. Sanz Seibert, J. T. Ross, J. W. Lucy, M. C. Safranski, T. J. Elsasser, T. H. Kahl, S. Rhoads, R. P. Baumgard, L. H. TI In utero heat stress increases postnatal core body temperature in pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT Beef Species Symposium Joint Annual Meeting CY JUL 20-24, 2014 CL Kansas City, MO SP Journal Anim Sci, Amer Soc Anim Sci DE in utero heat stress; pigs; thermal imprinting; thermoregulation ID METABOLIC-RATE; SHOCK-PROTEIN; PUMP ACTIVITY; EXPRESSION; HSP70; THERMOTOLERANCE; HYPERTHERMIA; TOLERANCE; NUTRITION; CATTLE AB In utero heat stress (IUHS) negatively impacts postnatal development, but how it alters future body temperature parameters and energetic metabolism is not well understood. Future body temperature indices and bioenergetic markers were characterized in pigs from differing in utero thermal environments during postnatal thermoneutral (TN) and cyclical heat stress (HS) exposure. First-parity pregnant gilts (n = 13) were exposed to 1 of 4 ambient temperature (T-a) treatments (HS [cyclic 28 degrees C to 34 degrees C] or TN [cyclic 18 degrees C to 22 degrees C]) applied for the entire gestation (HSHS, TNTN), HS for the first half of gestation (HSTN), or HS for the second half of gestation (TNHS). Twenty-four offspring (23.1 +/- 1.2 kg BW; n = 6 HSHS, n = 6 TNTN, n = 6 HSTN, n = 6 TNHS) were housed in TN (21.7 degrees C +/- 0.7 degrees C) conditions and then exposed to 2 separate but similar HS periods (HS1 = 6 d; HS2 = 6 d; cycling 28 degrees C to 36 degrees C). Core body temperature (T-core) was assessed every 15 min with implanted temperature recorders. Regardless of in utero treatment, Tcore increased during both HS periods (P = 0.01; 0.58 degrees C). During TN, HS1, and HS2, all IUHS pigs combined had increased Tcore (P = 0.01; 0.36 degrees C, 0.20 degrees C, and 0.16 degrees C, respectively) compared to TNTN controls. Although unaffected by in utero environment, the total plasma thyroxine to triiodothyronine ratio was reduced (P = 0.01) during HS1 and HS2 (39% and 29%, respectively) compared with TN. In summary, pigs from IUHS maintained an increased Tcore compared with TNTN controls regardless of external Ta, and this thermal differential may have practical implications to developmental biology and animal bioenergetics. C1 [Johnson, J. S.; Fernandez, M. V. Sanz; Seibert, J. T.; Ross, J. W.; Baumgard, L. H.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Lucy, M. C.; Safranski, T. J.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Elsasser, T. H.; Kahl, S.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Rhoads, R. P.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. EM baumgard@iastate.edu RI Rhoads, Robert/F-2861-2016 OI Rhoads, Robert/0000-0002-5205-5834 FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67003-30007] FX Results described herein were supported by the Agriculture and Food Research Initiative Competitive Grant number 2011-67003-30007 from the USDA National Institute of Food and Agriculture. NR 49 TC 0 Z9 0 U1 4 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD SEP PY 2015 VL 93 IS 9 BP 4312 EP 4322 DI 10.2527/jas.2015-9112 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CW7IR UT WOS:000365172300012 PM 26440331 ER PT J AU Foote, AP Hales, KE Kuehn, LA Keisler, DH King, DA Shackelford, SD Wheeler, TL Freetly, HC AF Foote, A. P. Hales, K. E. Kuehn, L. A. Keisler, D. H. King, D. A. Shackelford, S. D. Wheeler, T. L. Freetly, H. C. TI Relationship of leptin concentrations with feed intake, growth, and efficiency in finishing beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT Beef Species Symposium Joint Annual Meeting CY JUL 20-24, 2014 CL Kansas City, MO SP Journal Anim Sci, Amer Soc Anim Sci DE feed efficiency; growth; physiological markers ID CARCASS COMPOSITION; FAT-CONTENT; FACTOR-I; CATTLE; PLASMA; GENE; EXPRESSION; RUMINANTS; POLYMORPHISMS; ASSOCIATION AB The objective of this experiment was to determine the association of serum leptin concentrations with production measures including DMI, ADG, and G:F as well as carcass characteristics in genetically diverse finishing beef steers. Three cohorts of steers (n = 473 total) were individually fed a finishing ration for 92, 64, and 84 d for cohort 1, 2, and 3, respectively. Serum was collected on d 42, 22, and 19 of the experiment for cohort 1, 2, and 3, respectively. Leptin concentrations were positively correlated to DMI (r = 0.21, P < 0.01) but negatively correlated to grams DMI per kilogram initial BW (r = -0.21, P < 0.01). Leptin concentrations were also negatively correlated to ADG and G: F (P < 0.01). Leptin concentrations were positively correlated to 12th-rib fat thickness, yield grade, and marbling score (P < 0.01) and negatively correlated to LM area (P < 0.01). Using a mixed model analysis (SAS 9.3; SAS Inst. Inc., Cary, NC) to account for breed effects, leptin concentrations were positively associated with DMI (P = 0.01) and accounted for 1.10% of the variance. However, if initial BW and yield grade were included as covariates to account for body size and fatness, leptin was negatively associated with DMI (P = 0.02) and accounted for 0.54% of the variance. Regardless of covariates included in the model, leptin was negatively associated with ADG (P < 0.01) and G: F (P < 0.01) and accounted for 2.62 and 7.87% of the variance for ADG and G: F, respectively. Leptin concentrations were also positively associated with 12th-rib fat thickness, yield grade, and marbling score (P < 0.01) and accounted for 14.74, 12.74, and 6.99% of the variance for 12th-rib fat, yield grade, and marbling score, respectively. Leptin concentrations could be a useful physiological marker for growth and feed efficiency of finishing beef cattle. Genetic influences on the biology of leptin also need to be considered when using leptin as physiological marker for production measures. C1 [Foote, A. P.; Hales, K. E.; Kuehn, L. A.; King, D. A.; Shackelford, S. D.; Wheeler, T. L.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Keisler, D. H.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. EM Andrew.Foote@ars.usda.gov NR 21 TC 2 Z9 2 U1 0 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD SEP PY 2015 VL 93 IS 9 BP 4401 EP 4407 DI 10.2527/jas.2015-9339 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CW7IR UT WOS:000365172300021 PM 26440340 ER PT J AU Hall, DG Hentz, MG Patt, JM AF Hall, David G. Hentz, Matthew G. Patt, Joseph M. TI Behavioral Assay on Asian Citrus Psyllid Attraction to Orange Jasmine SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Diaphorina citri; huanglongbing; citrus greening; behavior assay ID GUAVA LEAF VOLATILES; DIAPHORINA-CITRI; HOST PLANTS; KUWAYAMA; DISEASE; HEMIPTERA; FLORIDA AB The Asian citrus psyllid (ACP) is an important pest because it transmits a bacterium putatively responsible for huanglongbing, a devastating citrus disease. Research on ACP chemical ecology is of interest with respect to identifying attractants and repellents for managing the psyllid. We report on an assay for investigating ACP attraction to the foliar odor of one of its host plants, orange jasmine (Murraya exotica L.). Flush shoots from this plant were placed into 25 dram vials covered with a paper wrapper, a lid with a small entrance hole was snapped onto each vial, the vials were placed into a small cage, adult ACP were released into the cage, and the location of the adults was determined 24 h later. A positive response required an adult to find and enter a vial. When single males or females were released, they were attracted into vials with flush 64 % of the time. At release rates of 25, 50 or 100 adults with 30 repetitions for each rate, relatively large mean percentages of adults (ca. 83 %) positively responded to flush shoots. ACP can escape from the assay vial and will do so if flush is not present. Working with ACP in empty vials, fluoropolymer resin applied to the inside of vials reduced escapes but did not eliminate them. An entrapment funnel for reducing escapes was tested, but it reduced ACP response rates. A good method of preventing escapes remains to be developed. When two vials of flush were placed in the assay cage and one was treated with a candidate repellent, only 8 % of adults settled in the vial with the repellent. C1 [Hall, David G.; Hentz, Matthew G.; Patt, Joseph M.] ARS, USDA, Ft Pierce, FL 34945 USA. RP Hall, DG (reprint author), ARS, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM david.hall@ars.usda.gov; matt.hentz@ars.usda.gov; joseph.patt@ars.usda.gov NR 24 TC 3 Z9 3 U1 3 U2 20 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD SEP PY 2015 VL 28 IS 5 BP 555 EP 568 DI 10.1007/s10905-015-9525-1 PG 14 WC Entomology SC Entomology GA CW7NO UT WOS:000365186100005 ER PT J AU Henry, IM Zinkgraf, MS Groover, AT Comai, L AF Henry, Isabelle M. Zinkgraf, Matthew S. Groover, Andrew T. Comai, Luca TI A System for Dosage-Based Functional Genomics in Poplar SO PLANT CELL LA English DT Article ID COPY NUMBER VARIATION; ARABIDOPSIS-THALIANA; GAMMA-IRRADIATION; POPULUS-TREMULOIDES; MOLECULAR CHARACTERIZATION; QUANTITATIVE TRAITS; INDUCED MUTATIONS; GENE-REGULATION; TRIPLOID MAIZE; POLLEN AB Altering gene dosage through variation in gene copy number is a powerful approach to addressing questions regarding gene regulation, quantitative trait loci, and heterosis, but one that is not easily applied to sexually transmitted species. Elite poplar (Populus spp) varieties are created through interspecific hybridization, followed by clonal propagation. Altered gene dosage relationships are believed to contribute to hybrid performance. Clonal propagation allows for replication and maintenance of meiotically unstable ploidy or structural variants and provides an alternative approach to investigating gene dosage effects not possible in sexually propagated species. Here, we built a genome-wide structural variation system for dosage-based functional genomics and breeding of poplar. We pollinated Populus deltoides with gamma-irradiated Populus nigra pollen to produce >500 F1 seedlings containing dosage lesions in the form of deletions and insertions of chromosomal segments (indel mutations). Using high-precision dosage analysis, we detected indel mutations in similar to 55% of the progeny. These indels varied in length, position, and number per individual, cumulatively tiling >99% of the genome, with an average of 10 indels per gene. Combined with future phenotype and transcriptome data, this population will provide an excellent resource for creating and characterizing dosage-based variation in poplar, including the contribution of dosage to quantitative traits and heterosis. C1 [Henry, Isabelle M.; Groover, Andrew T.; Comai, Luca] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. [Henry, Isabelle M.; Comai, Luca] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Zinkgraf, Matthew S.; Groover, Andrew T.] US Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. RP Comai, L (reprint author), Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. EM agroover@fs.fed.us; lcomai@ucdavis.edu OI Zinkgraf, Matthew/0000-0002-7229-8016; Groover, Andrew/0000-0002-6686-5774; Henry, Isabelle/0000-0002-6796-1119; Comai, Luca/0000-0003-2642-6619 FU NIH [S10RR029668, S10RR027303]; DOE Office of Science, Office of Biological and Environmental Research [DE-SC0007183] FX We thank Brian Stanton, Kathy Haiby, and Richard Shuren of Greenwood Resources for providing germplasm, performing controlled crosses, and providing extensive technical advice on poplar breeding and cultivation. We thank Annie Mix, Courtney Castle, Shani Sarena, and Suzanne Gerttula at the U.S. Forest Service Institute of Forest Genetics and Brian Watson at the University of Washington for assistance in poplar breeding and cultivation. We thank Clara Williams, Helen Tsai, and Jian Gao for technical assistance and Meric Lieberman and Kathie Ngo for bioinformatics assistance. We thank Karen Shaw at the Center for Comparative Medicine (UC Davis) for assistance with the irradiator. This work used the UC Davis DNA Technologies Core and the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303 for sequencing services. This research was supported by the DOE Office of Science, Office of Biological and Environmental Research, Grant DE-SC0007183. NR 75 TC 5 Z9 5 U1 13 U2 27 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD SEP PY 2015 VL 27 IS 9 BP 2370 EP 2383 DI 10.1105/tpc.15.00349 PG 14 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA CX0NP UT WOS:000365394100006 PM 26320226 ER PT J AU Cai, YQ Goodman, JM Pyc, M Mullen, RT Dyer, JM Chapman, KD AF Cai, Yingqi Goodman, Joel M. Pyc, Michal Mullen, Robert T. Dyer, John M. Chapman, Kent D. TI Arabidopsis SEIPIN Proteins Modulate Triacylglycerol Accumulation and Influence Lipid Droplet Proliferation SO PLANT CELL LA English DT Article ID ENDOPLASMIC-RETICULUM; PHYLOGENETIC ANALYSIS; GENE-EXPRESSION; ENERGY DENSITY; PLANT-LEAVES; OIL BODIES; YEAST; LIPODYSTROPHY; BIOGENESIS; EXPANSION AB The lipodystrophy protein SEIPIN is important for lipid droplet (LD) biogenesis in human and yeast cells. In contrast with the single SEIPIN genes in humans and yeast, there are three SEIPIN homologs in Arabidopsis thaliana, designated SEIPIN1, SEIPIN2, and SEIPIN3. Essentially nothing is known about the functions of SEIPIN homologs in plants. Here, a yeast (Saccharomyces cerevisiae) SEIPIN deletion mutant strain and a plant (Nicotiana benthamiana) transient expression system were used to test the ability of Arabidopsis SEIPINs to influence LD morphology. In both species, expression of SEIPIN1 promoted accumulation of large-sized lipid droplets, while expression of SEIPIN2 and especially SEIPIN3 promoted small LDs. Arabidopsis SEIPINs increased triacylglycerol levels and altered composition. In tobacco, endoplasmic reticulum (ER)-localized SEIPINs reorganized the normal, reticulated ER structure into discrete ER domains that colocalized with LDs. N-terminal deletions and swapping experiments of SEIPIN1 and 3 revealed that this region of SEIPIN determines LD size. Ectopic overexpression of SEIPIN1 in Arabidopsis resulted in increased numbers of large LDs in leaves, as well as in seeds, and increased seed oil content by up to 10% over wild-type seeds. By contrast, RNAi suppression of SEIPIN1 resulted in smaller seeds and, as a consequence, a reduction in the amount of oil per seed compared with the wild type. Overall, our results indicate that Arabidopsis SEIPINs are part of a conserved LD biogenesis machinery in eukaryotes and that in plants these proteins may have evolved specialized roles in the storage of neutral lipids by differentially modulating the number and sizes of lipid droplets. C1 [Cai, Yingqi; Chapman, Kent D.] Univ N Texas, Dept Biol Sci, Ctr Plant Lipid Res, Denton, TX 76203 USA. [Goodman, Joel M.] Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA. [Pyc, Michal; Mullen, Robert T.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Dyer, John M.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Chapman, KD (reprint author), Univ N Texas, Dept Biol Sci, Ctr Plant Lipid Res, Denton, TX 76203 USA. EM chapman@unt.edu OI Dyer, John/0000-0001-6215-0053; Pyc, Michal/0000-0001-8702-1661; Cai, Yingqi/0000-0002-0357-5809; Goodman, Joel/0000-0001-5483-202X; Chapman, Kent/0000-0003-0489-3072 FU U.S. Department of Energy, Division of Biological and Environmental Research [DE-FG02-09ER64812/DE-SC0000797]; NSF-MRI Grant [1126205]; National Institutes of Health [R01 GM084210]; American Diabetes Association [7-13-BS-055]; Natural Sciences and Engineering Research Council of Canada FX This research was supported by a grant from the U.S. Department of Energy, Division of Biological and Environmental Research (DE-FG02-09ER64812/DE-SC0000797). The Zeiss LSM710 confocal laser scanning microscope was acquired with funds from NSF-MRI Grant 1126205 to the University of North Texas (UNT). We thank Derk Binns at the University of Texas Southwestern Medical Center, Dallas, TX, for the yeast mutant strain ylr404w Delta, yeast expression plasmids pRS315-PGK and pRS315-Sc-SEIPIN, and advice regarding SEIPIN expression in transgenic yeast. We also thank David Garrett (UNT) for performing TEM of yeast cells, Lon Turnbull (UNT) for assistance with the confocal microscope, Bob Harris (Molecular and Cellular Imaging Facility, University of Guelph) for assistance with TEM of Arabidopsis mature seeds, and Qing Liu from CSIRO Plant Industry, Australia, for providing us with plant expression plasmids pORE04-LEC2 and pORE04-P19. K.D.C. is grateful to the National Science Foundation for providing individual research and development leave to assist in the supervision of this research and the preparation of this article by agreement under the Intergovernmental Personnel Act. J.M.G. was supported by funding provided by the National Institutes of Health (Grant R01 GM084210) and the American Diabetes Association (Grant 7-13-BS-055). R.T.M. was supported by funding provided by the Natural Sciences and Engineering Research Council of Canada. NR 65 TC 10 Z9 12 U1 2 U2 14 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD SEP PY 2015 VL 27 IS 9 BP 2616 EP 2636 DI 10.1105/tpc.15.00588 PG 21 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA CX0NP UT WOS:000365394100022 PM 26362606 ER PT J AU Bradford, SA Schijven, J Harter, T AF Bradford, Scott A. Schijven, Jack Harter, Thomas TI Microbial Transport and Fate in the Subsurface Environment: Introduction to the Special Section SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PATHOGENS; WATER; BIOREMEDIATION; REMOVAL; AQUIFER; VIRUSES AB Microorganisms constitute an almost exclusive form of life in the earth's subsurface environment ( not including caves), particularly at depths exceeding the soil horizon. While of broad interest to ecology and geology, scientific interest in the fate and transport of microorganisms, particularly those introduced through the anthropogenic environment, has focused on understanding the subsurface environment as a pathway for human pathogens and on optimizing the use of microbial organisms for remediation of potable groundwater. This special section, inspired by the 2014 Ninth International Symposium for Subsurface Microbiology, brings together recent efforts to better understand the spatiotemporal occurrence of anthropogenic microbial groundwater contamination and the fate and transport of microbes in the subsurface environment: in soils, deep unsaturated zones, and within aquifer systems. Work includes field reconnaissance, controlled laboratory studies to improve our understanding of specific fate and transport processes, and the development and application of improved mechanistic understanding of microbial fate and transport processes in the subsurface environment. The findings confirm and also challenge the limitations of our current understanding of highly complex microbial fate and transport processes across spatiotemporal scales in the subsurface environment; they also add to the increasing knowledge base to improve our ability to protect drinking water resources and perform in situ environmental remediation. C1 [Bradford, Scott A.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Schijven, Jack] Natl Inst Publ Hlth & Environm RIVM, Dept Stat Informat & Modelling, NL-3720 BA Bilthoven, Netherlands. [Schijven, Jack] Univ Utrecht, Fac Geosci, Dept Earth Sci, Utrecht, Netherlands. [Harter, Thomas] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. RP Bradford, SA (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM scott.bradford@ars.usda.gov FU International Society for Subsurface Microbiology FX The guest editors, Drs. Schijven and Harter, for this special collection chaired a session at the Ninth International Symposium on Subsurface Microbiology that was held in Pacific Grove, CA, on 5-10 Oct. 2014. This symposium was sponsored by the International Society for Subsurface Microbiology and administered by the National Water Research Institute. This special section evolved from discussions and presentations at this meeting. We would like to extend a special thanks to the authors, reviewers, Associate Editors, and staff at the Journal of Environmental Quality for their efforts to put together this high-quality special section in a timely manner. NR 45 TC 0 Z9 0 U1 7 U2 24 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD SEP-OCT PY 2015 VL 44 IS 5 BP 1333 EP 1337 DI 10.2134/jeq2015.07.0375 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA CW3SH UT WOS:000364911600001 PM 26436251 ER PT J AU Blaustein, RA Pachepsky, YA Shelton, DR Hill, RL AF Blaustein, Ryan A. Pachepsky, Yakov A. Shelton, Daniel R. Hill, Robert L. TI Release and Removal of Microorganisms from Land-Deposited Animal Waste and Animal Manures: A Review of Data and Models SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ESCHERICHIA-COLI O157-H7; CRYPTOSPORIDIUM-PARVUM OOCYSTS; ENTERICA SEROVAR TYPHIMURIUM; FECAL INDICATOR ORGANISMS; GRASS BUFFER CONDITIONS; CATTLE MANURE; OVERLAND-FLOW; POULTRY LITTER; UNITED-STATES; E. COLI AB Microbial pathogens present a leading cause of impairment to rivers, bays, and estuaries in the United States, and agriculture is often viewed as the major contributor to such contamination. Microbial indicators and pathogens are released from land-applied animal manure during precipitation and irrigation events and are carried in overland and subsurface flow that can reach and contaminate surface waters and ground water used for human recreation and food production. Simulating the release and removal of manure-borne pathogens and indicator microorganisms is an essential component of microbial fate and transport modeling regarding food safety and water quality. Although microbial release controls the quantities of available pathogens and indicators that move toward human exposure, a literature review on this topic is lacking. This critical review on microbial release and subsequent removal from manure and animal waste application areas includes sections on microbial release processes and release-affecting factors, such as differences in the release of microbial species or groups; bacterial attachment in turbid suspensions; animal source; animal waste composition; waste aging; manure application method; manure treatment effect; rainfall intensity, duration, and energy; rainfall recurrence; dissolved salts and temperature; vegetation and soil; and spatial and temporal scale. Differences in microbial release from liquid and solid manures are illustrated, and the influential processes are discussed. Models used for simulating release and removal and current knowledge gaps are presented, and avenues for future research are suggested. C1 [Blaustein, Ryan A.; Hill, Robert L.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Blaustein, Ryan A.; Pachepsky, Yakov A.; Shelton, Daniel R.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Pachepsky, YA (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090 NR 100 TC 8 Z9 8 U1 3 U2 21 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD SEP-OCT PY 2015 VL 44 IS 5 BP 1338 EP 1354 DI 10.2134/jeq2015.02.0077 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA CW3SH UT WOS:000364911600002 PM 26436252 ER PT J AU Leij, FJ Bradford, SA Wang, YS Sciortino, A AF Leij, Feike J. Bradford, Scott A. Wang, Yusong Sciortino, Antonella TI Langmuirian Blocking of Irreversible Colloid Retention: Analytical Solution, Moments, and Setback Distance SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SATURATED POROUS-MEDIA; BINARY ION-EXCHANGE; SOLUTION CHEMISTRY; REACTIVE SOLUTES; TRAVELING-WAVES; IDEAL MODEL; TRANSPORT; ADSORPTION; DEPOSITION; COLUMN AB Soil and aquifer materials have a finite capacity for colloid retention. Blocking of the limited number of available retention sites further decreases the rate of retention with time and enhances risks (e.g., pathogens or colloid-associated contaminants) or benefits (e.g., remediation by microorganisms or nanoparticles) of colloid migration. Our objective was to use a straightforward procedure, based on variable transformation and Laplace transform, to solve the problem of advective colloid transport with irreversible retention and Langmuirian blocking for a pulse-type condition. Formulas for the mean breakthrough time and retardation factor were obtained using zero-and first-order time moments of the breakthrough curves. Equations for the time and position (setback distance) for a particular colloid concentration were obtained from this information. Escherichia coli D21 g breakthrough curves and retention profiles in fine sand at four ionic strengths were well described by the model when parameters were optimized. Illustrative simulations demonstrated that blocking becomes more important for smaller retention capacity (S-m) and for larger retention rate coefficient (k), input concentration (C-o), and pulse duration. Blocking tended to delay colloid arrival time at a particular location relative to a conservative tracer, and produced larger setback distances for smaller k and S-m/C-o. C1 [Leij, Feike J.; Sciortino, Antonella] Calif State Univ Long Beach, Dept Civil Engn & Construct Engn Management, Long Beach, CA 90840 USA. [Bradford, Scott A.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Wang, Yusong] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. RP Leij, FJ (reprint author), Calif State Univ Long Beach, Dept Civil Engn & Construct Engn Management, 1250 Bellflower Blvd, Long Beach, CA 90840 USA. EM feike.leij@csulb.edu NR 37 TC 6 Z9 6 U1 2 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD SEP-OCT PY 2015 VL 44 IS 5 BP 1473 EP 1482 DI 10.2134/jeq2015.03.0147 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CW3SH UT WOS:000364911600014 PM 26436264 ER PT J AU Eugene, B Moore, PA Li, H Miles, D Trabue, S Burns, R Buser, M AF Eugene, Branly Moore, Philip A., Jr. Li, Hong Miles, Dana Trabue, Steven Burns, Robert Buser, Michael TI Effect of Alum Additions to Poultry Litter on In-House Ammonia and Greenhouse Gas Concentrations and Emissions SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID REDUCING PHOSPHORUS RUNOFF; CHEMICAL AMENDMENTS; ATMOSPHERIC AMMONIA; BROILER HOUSES; SALMONELLA POPULATIONS; CARBON-DIOXIDE; NITROUS-OXIDE; SULFATE; VOLATILIZATION; SOIL AB Alum [Al-2(SO4)(3)center dot 14H(2)O] addition to poultry litter has been shown to reduce ammonia (NH3) concentrations in poultry houses; however, its effects on greenhouse gas (GHG; N2O, CH4, and CO2) emissions is unknown. The objectives of this study were to determine the effects of alum additions on (i) in-house NH3 and GHG concentrations, (ii) NH3 and GHG emissions, and (iii) litter chemical properties. Two identical broiler houses located in northwest Arkansas were used for this study: one house was a control and the other was treated with alum between each flock of birds. Ventilation rates were coupled with in-house NH3 and GHG measurements to determine emission rates. Overall, alum additions significantly reduced the daily average in-house NH3 concentration by 42% (8.9 vs. 15.4 mu L L-1), and the overall NH3 emission rate was reduced by 47% (7.2 vs. 13.4 kg d(-1) house(-1)). The average cumulative NH3 emission for the three flocks was 330 kg house(-1) flock(-1) for the alum-treated house and 617 kg house(-1) flock(-1) for the control. Concentrations and emissions of nitrous oxide (N2O) and methane (CH4) from the alum-treated house were not significantly different than the untreated house. However, carbon dioxide (CO2) emissions were significantly higher from the untreated house than the alum-treated house. Alum also significantly increased litter N content and reduced the C/N ratio. These results indicate that the addition of alum to poultry litter is not only an effective management practice for reducing in-house NH3 concentrations and emissions but also significantly reduces CO2 emissions from poultry facilities. C1 [Eugene, Branly] Univ Arkansas, USDA ARS, Poultry Prod & Prod Safety Res Unit, Plant Sci 115, Fayetteville, AR 72701 USA. [Moore, Philip A., Jr.] USDA ARS, Fayetteville, AR 72701 USA. [Li, Hong] Univ Delaware, Newark, DE 19716 USA. [Miles, Dana] USDA ARS, Mississippi State, MS 39762 USA. [Trabue, Steven] USDA ARS, Ames, IA 50011 USA. [Burns, Robert] Univ Tennessee, Knoxville, TN 37996 USA. [Buser, Michael] Oklahoma State Univ, Stillwater, OK 74078 USA. RP Eugene, B (reprint author), Univ Arkansas, USDA ARS, Poultry Prod & Prod Safety Res Unit, Plant Sci 115, Fayetteville, AR 72701 USA. EM beugene@uark.edu NR 50 TC 3 Z9 3 U1 1 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD SEP-OCT PY 2015 VL 44 IS 5 BP 1530 EP 1540 DI 10.2134/jeq2014.09.0404 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CW3SH UT WOS:000364911600020 PM 26436270 ER PT J AU Dungan, RS Leytem, AB AF Dungan, Robert S. Leytem, April B. TI Detection of Purple Sulfur Bacteria in Purple and Non-purple Dairy Wastewaters SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SP-NOV.; MICROBIAL MAT; LAGOONS; LAKE; GAMMAPROTEOBACTERIUM; THIOCAPSA; SEDIMENT; ECOLOGY; COPPER; SOIL AB The presence of purple bacteria in manure storage lagoons is often associated with reduced odors. In this study, our objectives were to determine the occurrence of purple sulfur bacteria (PSB) in seven dairy wastewater lagoons and to identify possible linkages between wastewater properties and purple blooms. Community DNA was extracted from composited wastewater samples, and a conservative 16S rRNA gene sequence within Chromatiaceae and pufM genes found in both purple sulfur and nonsulfur bacteria was amplified. Analysis of the genes indicated that all of the lagoons contained sequences that were 92 to 97% similar with Thiocapsa roseopersicina. Sequences from a few lagoons were also found to be similar with other PSB, such as Marichromatium sp. (97%), Thiolamprovum pedioforme (93-100%), and Thiobaca trueperi (95-98%). pufM sequences amplified from enrichment and pure cultures were most similar to T. roseopersicina (93-96%). Carotenoid pigment concentrations, which were used as an indirect measure of purple bacteria levels in the wastewaters, were found to be positively correlated with salinity, nitrogen, total and volatile solids, and chemical oxygen demand; however, salinity could be the dominant factor influencing purple blooms. Due to the detection of PSB sequences in all lagoons, our findings suggest that the non-purple lagoons may have been purple in the past or may have the potential to become purple in the future. C1 [Dungan, Robert S.; Leytem, April B.] ARS, USDA, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. RP Dungan, RS (reprint author), ARS, USDA, Northwest Irrigat & Soils Res Lab, 3793 North 3600 East, Kimberly, ID 83341 USA. EM robert.dungan@ars.usda.gov NR 32 TC 1 Z9 1 U1 1 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD SEP-OCT PY 2015 VL 44 IS 5 BP 1550 EP 1555 DI 10.2134/jeq2015.03.0128 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA CW3SH UT WOS:000364911600022 PM 26436272 ER PT J AU Welch, KD Cook, D Green, BT Gardner, DR Pfister, JA McDaneld, TG Panter, KE AF Welch, Kevin D. Cook, Daniel Green, Benedict T. Gardner, Dale R. Pfister, James A. McDaneld, Tara G. Panter, Kip E. TI Adverse Effects of Larkspur (Delphinium spp.) on Cattle SO Agriculture-Basel LA English DT Review DE larkspur; delphinium; methyllycaconitine; cattle ID NICOTINIC ACETYLCHOLINE-RECEPTOR; TOXIC NORDITERPENOID ALKALOIDS; PRESSURE CHEMICAL-IONIZATION; ALPHA-BUNGAROTOXIN BINDING; TALL LARKSPUR; DITERPENOID ALKALOIDS; MASS-SPECTROMETRY; QUANTITATIVE-ANALYSIS; METHYLLYCACONITINE; NUTTALLIANUM AB There are numerous species of larkspur (Delphinium spp.) in North America. Larkspurs are a major cause of cattle losses on western ranges in the USA, especially on foothill and mountain rangelands. The toxicity of larkspur species is due to various norditerpenoid alkaloids. In this article, we review the current knowledge regarding larkspur ecology and distribution, analytical technologies to study and quantify the toxins in larkspur, the toxicology of the larkspur plants and their individual toxins, known genetic variations in larkspur susceptibility, and current management recommendations to mitigate losses from larkspur poisoning. C1 [Welch, Kevin D.; Cook, Daniel; Green, Benedict T.; Gardner, Dale R.; Pfister, James A.; Panter, Kip E.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. [McDaneld, Tara G.] USDA ARS, Meat Anim Res Ctr, Clay Ctr, NE 69833 USA. RP Welch, KD (reprint author), USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. EM Kevin.Welch@ars.usda.gov; Daniel.Cook@ars.usda.gov; Ben.Green@ars.usda.gov; Dale.Gardner@ars.usda.gov; Jim.Pfister@ars.usda.gov; Tara.McDaneld@ars.usda.gov; Kip.Panter@ars.usda.gov NR 79 TC 1 Z9 1 U1 1 U2 4 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD SEP PY 2015 VL 5 IS 3 BP 456 EP 474 DI 10.3390/agriculture5030456 PG 19 WC Agronomy SC Agriculture GA CV6IP UT WOS:000364374200007 ER PT J AU Buzby, JC Bentley, JT Padera, B Ammon, C Campuzano, J AF Buzby, Jean C. Bentley, Jeanine T. Padera, Beth Ammon, Cara Campuzano, Jennifer TI Estimated Fresh Produce Shrink and Food Loss in U.S. Supermarkets SO Agriculture-Basel LA English DT Article DE food loss; food waste; fruit; retail; shrink; supermarket; vegetables ID WASTE PREVENTION; UNITED-STATES; RETAIL; FRUIT; NANOTECHNOLOGY AB Data on fresh fruit and vegetable shrink in supermarkets is important to help understand where and how much shrink could potentially be reduced by supermarkets to increase their profitability. This study provides: (1) shrink estimates for 24 fresh fruits and 31 fresh vegetables in U.S. supermarkets in 2011 and 2012; and (2) retail-level food loss. For each covered commodity, supplier shipment data was aggregated from a sample of 2900 stores from one national and four regional supermarket retailers in the United States, and this sum was then compared with aggregated point-of-sale data from the same stores to estimate the amount of shrink by weight and shrink rates. The 2011-2012 average annual shrink rates for individual fresh vegetables varied from 2.2 percent for sweet corn to 62.9 percent for turnip greens and for individual fresh fruit ranged from 4.1 percent for bananas to 43.1 percent for papayas. When these shrink estimates were used in the Loss-Adjusted Food Availability data series, annual food loss for these commodities totaled 5.9 billion pounds of fresh fruit and 6.1 billion pounds of fresh vegetables. This study extends the literature by providing important information on where and how much shrink could potentially be reduced. Precise comparisons across studies are difficult. This information, combined with information on available and cost-effective technologies and practices, may help supermarkets target food loss reduction efforts though food loss will never be zero. C1 [Buzby, Jean C.; Bentley, Jeanine T.] USDA, Econ Res Serv, Washington, DC 20250 USA. [Padera, Beth] MobiSave, New York, NY 10019 USA. [Ammon, Cara] Beacon Res Solut, Chicago, IL 60640 USA. [Campuzano, Jennifer] Nielsen Perishables Grp Inc, Chicago, IL 60613 USA. RP Buzby, JC (reprint author), USDA, Econ Res Serv, 1400 Independence Ave,Mail Stop 1800 SW, Washington, DC 20250 USA. EM jbuzby@ers.usda.gov; jbentley@ers.usda.gov; BethPadera@hotmail.com; Cara@beaconresearchsolutions.com; Jennifer.Campuzano@nielsen.com NR 43 TC 4 Z9 4 U1 7 U2 21 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD SEP PY 2015 VL 5 IS 3 BP 626 EP 648 DI 10.3390/agriculture5030626 PG 23 WC Agronomy SC Agriculture GA CV6IP UT WOS:000364374200014 ER PT J AU Trippe, KM Griffith, SM Banowetz, GM Whitaker, GW AF Trippe, Kristin M. Griffith, Stephen M. Banowetz, Gary M. Whitaker, Gerald W. TI Biochars Derived from Gasified Feedstocks Increase the Growth and Improve Nutrient Acquisition of Triticum aestivum (L.) Grown in Agricultural Alfisols SO Agriculture-Basel LA English DT Article DE Triticum aestivum L; gasification; biochar; seed screenings; wheat; nutrition; acid soil; Kentucky bluegrass; aluminum toxicity ID CHEMICAL-CHARACTERIZATION; PACIFIC-NORTHWEST; ALUMINUM TOXICITY; SOIL; GASIFICATION; WHEAT; ASH AB Biochars are produced by low-oxygen gasification or pyrolysis of organic waste products, and can be co-produced with energy, achieving waste diversion and delivering a soil amendment that can improve agricultural yields. Although many studies have reported the agronomic benefits of biochars produced from pyrolysis, few have interrogated the ability of gasified biochars to improve crop productivity. An earlier study described the ability of a biochar that was derived from gasified Kentucky bluegrass (KB) seed screenings to impact the chemistry of acidic agricultural soils. However, that study did not measure the effects of the biochar amendment on plant growth or on nutrient acquisition. To quantify these effects we conducted a greenhouse study that evaluated wheat grown in agricultural soils amended with either the KB-based biochar or a biochar derived from a blend of woody mixed-waste. Our studies indicated that biochar amended soils promoted the growth of wheat in these agricultural alfisols. Our elemental analysis indicated that an attenuation of metal toxicity was likely responsible for the increased plant growth. The results of our study are placed in the context of our previous studies that characterized KB-sourced biochar and its effects on soil chemistry. C1 [Trippe, Kristin M.; Griffith, Stephen M.; Banowetz, Gary M.; Whitaker, Gerald W.] USDA ARS, Natl Forage Seed & Prod Res Ctr, Corvallis, OR 97331 USA. RP Trippe, KM (reprint author), USDA ARS, Natl Forage Seed & Prod Res Ctr, Corvallis, OR 97331 USA. EM Kristin.Trippe@ARS.USDA.GOV; smgriffith@me.com; banowetg@gmail.com; Jerry.Whittaker@ars.usda.gov NR 24 TC 1 Z9 1 U1 1 U2 3 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD SEP PY 2015 VL 5 IS 3 BP 668 EP 681 DI 10.3390/agriculture5030668 PG 14 WC Agronomy SC Agriculture GA CV6IP UT WOS:000364374200016 ER PT J AU Lima, IM Ro, KS Reddy, GB Boykin, DL Klasson, KT AF Lima, Isabel M. Ro, Kyoung S. Reddy, G. B. Boykin, Debbie L. Klasson, Kjell T. TI Efficacy of Chicken Litter and Wood Biochars and Their Activated Counterparts in Heavy Metal Clean up from Wastewater SO Agriculture-Basel LA English DT Article DE biochar; activated biochar; chicken litter; adsorption; copper; arsenic ID BLACK CARBON; ADSORPTIVE PROPERTIES; PYROLYSIS CHARACTERISTICS; CHEMICAL-PROPERTIES; SURFACE-CHEMISTRY; AQUEOUS-SOLUTION; BROILER MANURE; ESTIMATED COST; ALMOND SHELLS; BIO-OIL AB It is known that properties of activated biochars are tightly associated with those of the original feedstock as well as pyrolysis and activation conditions. This study examined two feedstock types, pine wood shavings and chicken litter, to produce biochars at two different pyrolysis temperatures and subsequently activated by steam, acid or base. In order to measure activation efficiency, all materials were characterized for their properties and ability to remediate two well-known heavy metals of concern: copper and arsenic. Base activated biochars were superior in arsenic adsorption, to acid or steam activated samples, but increase in adsorption was not significant to warrant use. For wood biochars, significant increases of surface functionality as related to oxygen bearing groups and surface charge were observed upon acid activation which led to increased copper ion adsorption. However, oxygen bearing functionalities were not sufficient to explain why chicken litter biochars and steam activated biochars appeared to be significantly superior to wood shavings in positively charged metal ion adsorption. For chicken litter, functionality of respective biochars could be related to phosphate containing groups inherited from feedstock composition, favorably positioning this feedstock in metal ion remediation applications. C1 [Lima, Isabel M.; Klasson, Kjell T.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Ro, Kyoung S.] ARS, USDA, Costal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Reddy, G. B.] North Carolina A&T State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA. [Boykin, Debbie L.] ARS, USDA, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. RP Lima, IM (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM isabel.lima@ars.usda.gov; kyoung.ro@ars.usda.gov; reddyg@ncat.edu; Debbie.Boykin@ars.usda.gov; thomas.klasson@ars.usda.gov NR 59 TC 2 Z9 2 U1 6 U2 14 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD SEP PY 2015 VL 5 IS 3 BP 806 EP 825 DI 10.3390/agriculture5030806 PG 20 WC Agronomy SC Agriculture GA CV6IP UT WOS:000364374200024 ER PT J AU Zhao, DL Davidson, RW Baltazar, M Comstock, JC McCord, P Sood, S AF Zhao, Duli Davidson, R. Wayne Baltazar, Miguel Comstock, Jack C. McCord, Per Sood, Sushma TI Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program SO Agronomy-Basel LA English DT Article DE sugarcane; Canal Point (CP) sugarcane breeding program; brown rust; screening for rust resistance ID RESISTANCE GENE; REGISTRATION; YIELD; CULTIVARS; BIOMASS; PROGENY AB Sugarcane (Saccharum spp.) brown rust (caused by Puccinia melanocephala Syd. & P. Syd) was first reported in the United States in 1978 and is still one of the great challenges for sugarcane production. A better understanding of sugarcane genotypic variation in response to brown rust will help optimize breeding and selection strategies for disease resistance. Brown rust ratings were scaled from non-infection (0) to severe infection (4) with intervals of 0.5 and routinely recorded for genotypes in the first clonal selection stage of the Canal Point sugarcane breeding program in Florida. Data were collected from 14,272 and 12,661 genotypes and replicated check cultivars in 2012 and 2013, respectively. Mean rust rating, % infection, and severity in each family and progeny of female parent were determined, and their coefficients of variation (CV) within and among families (females) were estimated. Considerable variation exists in rust ratings among families or females. The families and female parents with high susceptibility or resistance to brown rust were identified and ranked. The findings of this study can help scientists to evaluate sugarcane crosses and parents for brown rust disease, to use desirable parents for crossing, and to improve genetic resistance to brown rust in breeding programs. C1 [Zhao, Duli; Comstock, Jack C.; McCord, Per; Sood, Sushma] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Davidson, R. Wayne; Baltazar, Miguel] Florida Sugar Cane League Inc, Clewiston, FL 33440 USA. RP Zhao, DL (reprint author), USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. EM duli.zhao@ars.usda.gov; wayne.davidson@ars.usda.gov; miguel.baltazar@ars.usda.gov; jack.comstock@ars.usda.gov; per.mccord@ars.usda.gov; Sushma.sood@ars.usda.gov NR 43 TC 1 Z9 1 U1 2 U2 2 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD SEP PY 2015 VL 5 IS 3 BP 341 EP 362 DI 10.3390/agronomy5030341 PG 22 WC Agronomy SC Agriculture GA CV5MV UT WOS:000364315800005 ER PT J AU Baker, JT Gitz, DC Stout, JE Lascano, RJ AF Baker, Jeffrey T. Gitz, Dennis C. Stout, John E. Lascano, Robert J. TI Cotton Water Use Efficiency under Two Different Deficit Irrigation Scheduling Methods SO Agronomy-Basel LA English DT Article DE deficit irrigation; canopy temperature; cotton; yield; water use efficiency ID CANOPY TEMPERATURE-MEASUREMENTS; POTENTIAL DEPRESSIONS; INFRARED THERMOMETRY; STRESS INDEX; LINT YIELD; PRODUCTIVITY; DROUGHT; WHEAT; PHOTOSYNTHESIS; RICE AB Declines in Ogallala aquifer levels used for irrigation has prompted research to identify methods for optimizing water use efficiency (WUE) of cotton (Gossypium hirsutum L). In this experiment, conducted at Lubbock, TX, USA in 2014, our objective was to test two canopy temperature based stress indices, each at two different irrigation trigger set points: the Stress Time (ST) method with irrigation triggers set at 5.5 (ST_5.5) and 8.5 h (ST_8.5) and the Crop Water Stress Index (CWSI) method with irrigation triggers set at 0.3 (CWSI_0.3) and 0.6 (CWSI_0.6). When these irrigation triggers were exceeded on a given day, the crop was deficit irrigated with 5 mm of water via subsurface drip tape. Also included in the experimental design were a well-watered (WW) control irrigated at 110% of potential evapotranspiration and a dry land (DL) treatment that relied on rainfall only. Seasonal crop water use ranged from 353 to 625 mm across these six treatments. As expected, cotton lint yield increased with increasing crop water use but lint yield WUE displayed asignificant (p 0.05) peak near 3.6 to 3.7 kg ha(-1) mm(-1) for the ST_5.5 and CWSI_0.3 treatments, respectively. Our results suggest that WUE may be optimized in cotton with less water than that needed for maximum lint yield. C1 [Baker, Jeffrey T.] USDA ARS, Plant Stress & Water Conservat Lab, Big Spring, TX 79720 USA. [Gitz, Dennis C.; Stout, John E.; Lascano, Robert J.] USDA ARS, Plant Stress & Water Conservat Lab, Lubbock, TX 79415 USA. RP Baker, JT (reprint author), USDA ARS, Plant Stress & Water Conservat Lab, 302 West I-20, Big Spring, TX 79720 USA. EM Jeff.Baker@ars.usda.gov; dennis.gitz@ars.usda.gov; john.stout@ars.usda.gov; robert.lascano@ars.usda.gov FU Ogallala Aquifer Program; USDA-Agricultural Research Service; Kansas State University; Texas AgriLife Research; Texas AgriLife Extension Service; Texas Tech University; West Texas AM University FX The technical support of Charles Yates, Cody Byars and Bill Webb in conducting these experiments is gratefully acknowledged. This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas AgriLife Research, Texas AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 33 TC 0 Z9 0 U1 2 U2 6 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND EI 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD SEP PY 2015 VL 5 IS 3 BP 363 EP 373 DI 10.3390/agronomy5030363 PG 11 WC Agronomy SC Agriculture GA CV5MV UT WOS:000364315800006 ER PT J AU Skinner, C Zhang, GD Patfield, S He, XH AF Skinner, Craig Zhang, Guodong Patfield, Stephanie He, Xiaohua TI An In Vitro Combined Antibiotic-Antibody Treatment Eliminates Toxicity from Shiga Toxin-Producing Escherichia coli SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID HEMOLYTIC-UREMIC SYNDROME; LACTAMASE-PRODUCING ENTEROBACTERIACEAE; CLOSTRIDIUM-DIFFICILE INFECTION; UNITED-STATES; MONOCLONAL-ANTIBODIES; TIGECYCLINE; O157-H7; STRAINS; GENE; IDENTIFICATION AB Treating Shiga toxin-producing Escherichia coli (STEC) gastrointestinal infections is difficult. The utility of antibiotics for STEC treatment is controversial, since antibiotic resistance among STEC isolates is widespread and certain antibiotics dramatically increase the expression of Shiga toxins (Stxs), which are some of the most important virulence factors in STEC. Stxs contribute to life-threatening hemolytic uremic syndrome (HUS), which develops in considerable proportions of patients with STEC infections. Understanding the antibiotic resistance profiles of STEC isolates and the Stx induction potential of promising antibiotics is essential for evaluating any antibiotic treatment of STEC. In this study, 42 O157:H7 or non-O157 STEC isolates (including the "big six" serotypes) were evaluated for their resistance against 22 antibiotics by using an antibiotic array. Tigecycline inhibited the growth of all of the tested STEC isolates and also inhibited the production of Stxs (Stx2 in particular). In combination with neutralizing antibodies to Stx1 and Stx2, the tigecycline-antibody treatment fully protected Vero cells from Stx toxicity, even when the STEC bacteria and the Vero cells were cultured together. The combination of an antibiotic such as tigecycline with neutralizing antibodies presents a promising strategy for future STEC treatments. C1 [Skinner, Craig; Patfield, Stephanie; He, Xiaohua] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Zhang, Guodong] US FDA, Ctr Food Safety & Appl Nutr, Div Microbiol, College Pk, MD USA. RP He, XH (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM xiaohua.he@ari.usda.gov FU USDA-ARS National Program [NP108, 5325-42000-048-00D] FX This project was supported by USDA-ARS National Program NP108 (CRIS project 5325-42000-048-00D). NR 44 TC 1 Z9 1 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0066-4804 EI 1098-6596 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD SEP PY 2015 VL 59 IS 9 BP 5435 EP 5444 DI 10.1128/AAC.00763-15 PG 10 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA CV5XD UT WOS:000364343900043 PM 26100707 ER PT J AU Mollenkopf, DF Faubel, RL Pancholi, P Landers, TF Erdman, MM Daniels, JB Wittum, TE AF Mollenkopf, Dixie F. Faubel, Roger L. Pancholi, Preeti Landers, Timothy F. Erdman, Matthew M. Daniels, Joshua B. Wittum, Thomas E. TI Surveillance and Characterization of Carbapenemase-Producing Klebsiella pneumoniae Recovered from Patient Stool Samples at a Tertiary Care Medical Center SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Letter ID ENTEROBACTERIACEAE; EPIDEMIOLOGY C1 [Mollenkopf, Dixie F.; Wittum, Thomas E.] Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA. [Faubel, Roger L.; Pancholi, Preeti] Ohio State Univ, Wexner Med Ctr, Dept Pathol, Columbus, OH 43210 USA. [Landers, Timothy F.] Ohio State Univ, Coll Nursing, Columbus, OH 43210 USA. [Erdman, Matthew M.] USDA, Diagnost Bacteriol Lab, NVSL, Ames, IA USA. [Daniels, Joshua B.] Ohio State Univ, Coll Vet Med, Dept Vet Clin Sci, Columbus, OH 43210 USA. RP Wittum, TE (reprint author), Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA. EM wittum.1@osu.edu OI Landers, Timothy/0000-0003-3269-664X NR 7 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0066-4804 EI 1098-6596 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD SEP PY 2015 VL 59 IS 9 BP 5857 EP 5859 DI 10.1128/AAC.01085-15 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA CV5XD UT WOS:000364343900100 PM 26100704 ER PT J AU Cho, SH Choi, S Le, NH Kim, SJ Hwang, H AF Cho, S. H. Choi, S. Le, N. H. Kim, S. J. Hwang, H. TI ROBUST LEAN TISSUE SEGMENTATION FOR BEEF QUALITY GRADING SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Beef quality; Color computer vision; Entropy threshold; Interactive processing; Lean tissue boundary AB To automatically evaluate beef quality, a system must be developed to properly segment lean tissue in a sectional image of the 13th beef rib. To this end, a mobile color computer vision system and its corresponding image processing algorithms were developed for on-site application. The algorithms implement Renyi entropy and a texture index to provide adaptive thresholding. Automatic smoothing and modification followed boundary extraction, and binary morphological approaches were also taken. When 54 images of beef cut samples were assessed without manual intervention, the proposed algorithms exhibited an average boundary extraction error of 3% and an average pixel distance error of 1.8 pixels relative to assessments made by a human expert. The computing time for these sample images was of approximately 5.5 s. In addition, a user-friendly interactive man-machine interface was also developed to allow for a human expert to modin) the extracted boundary. C1 [Cho, S. H.] Agri Machinery Certificat Team, Suwon, Gyeonggi Do, South Korea. [Choi, S.] ARS, USDA, Russell Res Ctr, Athens, GA USA. [Le, N. H.] Eastern Int Univ, Mechatron Dept, Binh Duong, Vietnam. [Kim, S. J.] Sungkyunkwan Univ, Coll Biotechnol & Bioengn, Dept Biomechatron Engn, Suwon, South Korea. [Hwang, H.] Sungkyunkwan Univ, Coll Biotechnol & Bioengn, Dept Biomechatron Engn, Suwon 440746, Gyeonggi Do, South Korea. RP Hwang, H (reprint author), Sungkyunkwan Univ, Coll Biotechnol & Bioengn, Dept Biomechatron Engn, 109 Seobu Ro, Suwon 440746, Gyeonggi Do, South Korea. EM hhwang@skku.edu FU Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries(IPET) through Technology Commercialization Support Program; Ministry of Agriculture, Food and Rural Affairs(MAFRA) [P430-006] FX This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries(IPET) through Technology Commercialization Support Program, funded by Ministry of Agriculture, Food and Rural Affairs(MAFRA)(P430-006). NR 13 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD SEP PY 2015 VL 31 IS 5 BP 809 EP 823 PG 15 WC Agricultural Engineering SC Agriculture GA CV9JJ UT WOS:000364603200014 ER PT J AU Cleavitt, NL Hinds, JW Poirot, RL Geiser, LH Dibble, AC Leon, B Perron, R Pardo, LH AF Cleavitt, Natalie L. Hinds, James W. Poirot, Richard L. Geiser, Linda H. Dibble, Alison C. Leon, Bennet Perron, Ralph Pardo, Linda H. TI Epiphytic macrolichen communities correspond to patterns of sulfur and nitrogen deposition in the northeastern United States SO BRYOLOGIST LA English DT Article DE Thallus condition; cumulative deposition; air pollution; epiphyte; cyanolichen; sensitivity rating ID RAMALINA-MENZIESII TAYL; ACADIA NATIONAL-PARK; CRITICAL LOADS; AIR-POLLUTION; ATMOSPHERIC DEPOSITION; SOUTHERN-CALIFORNIA; LICHEN COMMUNITIES; GLOBAL ASSESSMENT; NORTH-AMERICA; FORESTS AB Atmospheric deposition of sulfur (S) and nitrogen (N) has decreased steadily in the northeastern U.S. since the federal 1970 Clean Air Act was passed, yet deposition remains elevated above natural background levels throughout the region. Epiphytic macrolichens are highly sensitive to air pollution and their status is a good indicator of ecological health. We used deposition modeling for 2000-2013 and multiple metrics of lichen status (i.e., species composition, species richness, thallus condition, lichen sensitivity indices, lichen elemental analysis) to assess air pollution effects at 24 plots in four federally-mandated Class I areas. The areas (Lye Brook Wilderness, VT; Great Gulf and the Presidential Range-Dry River Wildernesses, NH; and Acadia National Park, ME) encompass a range of high to low deposition sites. We developed thallus condition scores and sensitivity groups and indices for S and N based on species patterns using deposition estimates gleaned from a larger, independent data base. Non-metric multidimensional scaling ordinations differentiated forest structure effects on lichen community composition from more complex deposition and elevation effects. Annual mean and cumulative deposition of N correlated strongly with decreases in lichen species richness and N-sensitive species, and poorer thallus condition. Cumulative dry deposition of S yielded the best fit to decreases in thallus condition, poorer community-based S Index values, and absence of many S-sensitive species. Multiple metrics provided consistent evidence that higher depositional loading was associated with greater adverse effects. In general, stronger correlations between present day lichen metrics and cumulative deposition (post-2000), compared to current deposition, emphasize the long-term nature of emissions impacts and continued need to control S and N emissions to restore the ecological health of lichen communities and linked biota. C1 [Cleavitt, Natalie L.] Cornell Univ, Dept Nat Resources, Ithaca, NY 14853 USA. [Hinds, James W.; Dibble, Alison C.] Univ Maine, Sch Biol & Ecol, Orono, ME 04469 USA. [Poirot, Richard L.; Leon, Bennet] Vermont Dept Environm Conservat, Air Qual & Climate Div, Montpelier, VT 05620 USA. [Geiser, Linda H.] US Forest Serv, Watershed Fish Wildlife Air & Rare Plants, Washington, DC 20250 USA. [Perron, Ralph] US Forest Serv, Eastern Reg Air Program, Campton, NH 03223 USA. [Pardo, Linda H.] US Forest Serv, No Res Stn, Burlington, VT 05405 USA. RP Cleavitt, NL (reprint author), Cornell Univ, Dept Nat Resources, Fernow Hall, Ithaca, NY 14853 USA. EM nlc4@cornell.edu FU U.S. Forest Service; National Park Research Fellowship; Acadia Partners for Science and Research grant - L.L. Bean; National Park Service; Hubbard Brook NSF-LTER grant [DEB-1114804] FX Funding was provided by the U.S. Forest Service (National Air Program, WMNF and GMNF) to ACD and NLC; National Park Research Fellowship to NLC; Acadia Partners for Science and Research grant sponsored by L.L. Bean to NLC and ACD; and the National Park Service to LHP. Data analysis and writing by NLC was funded by a Hubbard Brook NSF-LTER grant (DEB-1114804) to Timothy Fahey. We thank David Werier, Howard Prescott, Maurane Bourjouin, Stephanie Freeman, Erin Lane, Stacy Lemieux, Mary Beth Poli, Sarah Thorne and Anne Rendall for field assistance. David Manski, William Gawley and Kathleen Weathers aided with plot selection and logistics in Acadia. Cliff Wetmore, Pam Templer, Johnny Boggs and Steve McNulty helped with relocating their study plots. Scott Copeland, Stephen Clayden and two anonymous reviewers provided thoughtful reviews of previous versions of this paper. Louise Roy helped in many ways, including cleaning lichens for elemental analysis. NR 57 TC 1 Z9 1 U1 8 U2 22 PU AMER BRYOLOGICAL LICHENOLOGICAL SOC INC PI OMAHA PA C/O DR ROBERT S EGAN, SEC-TRES, ABLS, UNIV NEBRASKA OMAHA, DEPT BIOLOGY, OMAHA, NE 68182-0040 USA SN 0007-2745 EI 1938-4378 J9 BRYOLOGIST JI Bryologist PD FAL PY 2015 VL 118 IS 3 BP 304 EP 324 DI 10.1639/0007-2745-118.3.304 PG 21 WC Plant Sciences SC Plant Sciences GA CV7FZ UT WOS:000364439100008 ER PT J AU Pinto, LC Zinn, YL de Mello, CR Owens, PR Norton, LD Curi, N AF Pinto, Leandro Campos Zinn, Yuri Lopes de Mello, Carlos Rogerio Owens, Philip Ray Norton, Lloyd Darrell Curi, Nilton TI MICROMORPHOLOGY AND PEDOGENESIS OF MOUNTAINOUS INCEPTISOLS IN THE MANTIQUEIRA RANGE (MG) SO CIENCIA E AGROTECNOLOGIA LA English DT Article DE Soil formation; soil microstructure; land use change; forest soils ID MINAS-GERAIS; ATLANTIC FOREST; HIGHLAND SOILS; LAND-USE; BRAZIL; ATTRIBUTES; KNOWLEDGE; CALABRIA; STATE AB Understanding soil formation processes across different landscapes is needed to predict how soil properties will respond to land use change. This study aimed to characterize mountainous Inceptisols (Cambisols) under high altitude subtropical climate in southeastern Brazil, by soil physical, chemical and micromorphological analyses, under native forest and pasture. The soil under pasture had a greater bulk density than under forest, resulting in a severe reduction of macroporosity. At two depths, coarse quartz grains are angular, suggesting absence of transportational processes, thus confirming an autochthonous pedogenesis from the underlying gneissic rock. Most feldspars were weathered beyond recognition, but mineral alteration was commonly seen across cleavage plans and edges of micas. The micromorphological results suggest an intermediate stage of mineral weathering and soil development, which is in accordance with properties expected to be found in Inceptisols. C1 [Pinto, Leandro Campos; Zinn, Yuri Lopes; Curi, Nilton] Univ Fed Lavras UFLA, DCS, BR-37200000 Lavras, MG, Brazil. [de Mello, Carlos Rogerio] Univ Fed Lavras UFLA, Dept Engn DEG, BR-37200000 Lavras, MG, Brazil. [Owens, Philip Ray] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Pinto, LC (reprint author), Univ Fed Lavras UFLA, DCS, Cx P 3037, BR-37200000 Lavras, MG, Brazil. EM leandcampos@yahoo.com.br RI Zinn, Yuri/A-6947-2013 OI Zinn, Yuri/0000-0001-5105-7996 NR 35 TC 2 Z9 2 U1 1 U2 13 PU UNIV FEDERAL LAVRAS-UFLA PI LAVRAS PA CAIXA POSTAL 3037, LAVRAS, MG 37200-000, BRAZIL SN 1413-7054 EI 1981-1829 J9 CIENC AGROTEC JI Cienc. Agrotec. PD SEP-OCT PY 2015 VL 39 IS 5 BP 455 EP 462 DI 10.1590/S1413-70542015000500004 PG 8 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA CW0VT UT WOS:000364708600004 ER PT J AU Grace, MH Truong, AN Truong, VD Raskin, I Lila, MA AF Grace, Mary H. Truong, An N. Van-Den Truong Raskin, Ilya Lila, Mary Ann TI Novel value-added uses for sweet potato juice and flour in polyphenol- and protein-enriched functional food ingredients SO FOOD SCIENCE & NUTRITION LA English DT Article DE Anti-inflammatory; antioxidant; berries; polyphenols; stability; sweet potato ID BETA-CAROTENE; OXIDATIVE STRESS; ASCORBIC-ACID; D-GALACTOSE; ANTHOCYANINS; ANTIOXIDANT; FRUIT; VEGETABLES; MICE; PREVENTION AB Blackcurrant, blueberry, and muscadine grape juices were efficiently sorbed, concentrated, and stabilized into dry granular ingredient matrices which combined anti-inflammatory and antioxidant fruit polyphenols with sweet potato functional constituents (carotenoids, vitamins, polyphenols, fibers). Total phenolics were highest in blackcurrant-orange sweet potato ingredient matrices (34.03 mg/g), and lowest in muscadine grape-yellow sweet potato matrices (10.56 mg/g). Similarly, anthocyanins were most concentrated in blackcurrant-fortified orange and yellow sweet potato matrices (5.40 and 6.54 mg/g, respectively). Alternatively, other protein-rich edible matrices (defatted soy flour, light roasted peanut flour, and rice protein concentrate) efficiently captured polyphenols (6.09-9.46 mg/g) and anthocyanins (0.77-1.27 mg/g) from purple-fleshed sweet potato juice, with comparable efficiency. Antioxidant activity correlated well with total phenolic content. All formulated ingredient matrices stabilized and preserved polyphenols for up to 24 weeks, even when stored at 37 degrees C. Complexation with juice-derived polyphenols did not significantly alter protein or carbohydrate profiles of the matrices. Sensory evaluation of the ingredient matrices suggested potential uses for a wide range of functional food products. C1 [Grace, Mary H.; Lila, Mary Ann] N Carolina State Univ, Plants Human Hlth Inst, Dept Food Bioproc & Nutr Sci, Kannapolis, NC 28081 USA. [Truong, An N.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Van-Den Truong] N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Raskin, Ilya] Rutgers State Univ, Dept Plant Biol & Pathol, SEBS, New Brunswick, NJ 08901 USA. RP Lila, MA (reprint author), N Carolina State Univ, Plants Human Hlth Inst, Dept Food Bioproc & Nutr Sci, North Carolina Res Campus, Kannapolis, NC 28081 USA. EM maryann_lila@ncsu.edu NR 35 TC 2 Z9 2 U1 4 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2048-7177 J9 FOOD SCI NUTR JI Food Sci. Nutr. PD SEP PY 2015 VL 3 IS 5 BP 415 EP 424 DI 10.1002/fsn3.234 PG 10 WC Food Science & Technology SC Food Science & Technology GA CW1NQ UT WOS:000364757500006 PM 26405527 ER PT J AU Cameron, A Huynh, S Scott, NLE Frirdich, E Apel, D Foster, LJ Parker, CT Gaynor, EC AF Cameron, Andrew Huynh, Steven Scott, Nichol Las E. Frirdich, Emilisa Apel, Dmitry Foster, Leonard J. Parker, Craig T. Gaynor, Erin C. TI High-Frequency Variation of Purine Biosynthesis Genes Is a Mechanism of Success in Campylobacter jejuni SO MBIO LA English DT Article ID GLUTAMINE 5-PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE; ESCHERICHIA-COLI; INORGANIC POLYPHOSPHATE; MUTATION-RATES; ADENINE PHOSPHORIBOSYLTRANSFERASE; SALMONELLA-TYPHIMURIUM; RESPONSE REGULATOR; PHASE; BACTERIA; SURVIVAL AB Phenotypic variation is prevalent in the zoonotic pathogen Campylobacter jejuni, the leading agent of enterocolitis in the developed world. Heterogeneity enhances the survival and adaptive malleability of bacterial populations because variable phenotypes may allow some cells to be protected against future stress. Exposure to hyperosmotic stress previously revealed prevalent differences in growth between C. jejuni strain 81-176 colonies due to resistant or sensitive phenotypes, and these isolated colonies continued to produce progeny with differential phenotypes. In this study, whole-genome sequencing of isolated colonies identified allelic variants of two purine biosynthesis genes, purF and apt, encoding phosphoribosyltransferases that utilize a shared substrate. Genetic analyses determined that purF was essential for fitness, while apt was critical. Traditional and high-depth amplicon-sequencing analyses confirmed extensive intrapopulation genetic variation of purF and apt that resulted in viable strains bearing alleles with in-frame insertion duplications, deletions, or missense polymorphisms. Different purF and apt alleles were associated with various stress survival capabilities under several niche-relevant conditions and contributed to differential intracellular survival in an epithelial cell infection model. Amplicon sequencing revealed that intracellular survival selected for stress-fit purF and apt alleles, as did exposure to oxygen and hyperosmotic stress. Putative protein recognition direct repeat sequences were identified in purF and apt, and a DNA-protein affinity screen captured a predicted exonuclease that promoted the global spontaneous mutation rate. This work illustrates the adaptive properties of high-frequency genetic variation in two housekeeping genes, which influences C. jejuni survival under stress and promotes its success as a pathogen. IMPORTANCE C. jejuni is an important cause of bacterial diarrheal illness. Bacterial populations have many strategies for stress survival, but phenotypic variation due to genetic diversity has a powerful advantage: no matter how swift the change in environment, a fraction of the population already expresses the survival trait. Nonclonality is thus increasingly viewed as a mechanism of population success. Our previous work identified prominent resistant/sensitive colonial variation in C. jejuni bacteria in response to hyperosmotic stress; in the work presented here, we attribute that to high-frequency genetic variation in two purine biosynthesis genes, purF and apt. We demonstrated selective pressure for nonlethal mutant alleles of both genes, showed that single-cell variants had the capacity to give rise to diverse purF and apt populations, and determined that stress exposure selected for desirable alleles. Thus, a novel C. jejuni adaptive strategy was identified, which was, unusually, reliant on prevalent genetic variation in two housekeeping genes. C1 [Cameron, Andrew; Frirdich, Emilisa; Apel, Dmitry; Gaynor, Erin C.] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V5Z 1M9, Canada. [Huynh, Steven; Parker, Craig T.] USDA, Western Reg Res Ctr, Produce Safety & Microbiol Unit, Albany, CA 94710 USA. [Scott, Nichol Las E.; Foster, Leonard J.] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC V5Z 1M9, Canada. [Scott, Nichol Las E.; Foster, Leonard J.] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V5Z 1M9, Canada. RP Gaynor, EC (reprint author), Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V5Z 1M9, Canada. EM gaynor@mail.ubc.ca OI Scott, Nichollas/0000-0003-2556-8316 FU Canadian Institutes of Health Research (CIHR) [MOP 68981, MOP-77688]; Burroughs Wellcome Fund; USDA Agricultural Research Service CRIS [5325-42000-047]; National Health and Medical Research Council of Australia Overseas Biomedical fellowship [APP1037373]; Michael Smith Foundation for Health Research postdoctoral fellowship [5363]; CIHR Canada graduate doctoral scholarship FX This project was supported by Canadian Institutes of Health Research (CIHR) grants MOP 68981 to E.C.G. and MOP-77688 to L.J.F., by the Burroughs Wellcome Fund (E.C.G.), and USDA Agricultural Research Service CRIS project 5325-42000-047 to C.T.P. N.E.S was supported by a National Health and Medical Research Council of Australia Overseas Biomedical fellowship (no. APP1037373) and a Michael Smith Foundation for Health Research postdoctoral fellowship (no. 5363). A.C. is supported by a CIHR Canada graduate doctoral scholarship. NR 80 TC 1 Z9 1 U1 2 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD SEP-OCT PY 2015 VL 6 IS 5 AR e00612-15 DI 10.1128/mBio.00612-15 PG 15 WC Microbiology SC Microbiology GA CV8HK UT WOS:000364523100002 PM 26419875 ER PT J AU Cadete, RM Cheab, MAM Santos, RO Safar, SVB Zilli, JE Vital, MJS Basso, LC Lee, CF Kurtzman, CP Lachance, MA Rosa, CA AF Cadete, Raquel M. Cheab, Monaliza A. M. Santos, Renata O. Safar, Silvana V. B. Zilli, Jerri E. Vital, Marcos J. S. Basso, Luiz C. Lee, Ching-Fu Kurtzman, Cletus P. Lachance, Marc-Andre Rosa, Carlos A. TI Cyberlindnera xylosilytica sp nov., a xylitol-producing yeast species isolated from lignocellulosic materials SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID THAILAND; SOIL AB Independent surveys of yeasts associated with lignocellulosic-related materials led to the discovery of a novel yeast species belonging to the Cyberlindnera clade (Saccharomycotina, Ascomycota). Analysis of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene showed that this species is related to C. japonica, C. maesa and C. easanensis. Six isolates were obtained from different sources, including rotting wood, tree bark and sugar cane filter cake in Brazil, frass from white oak in the USA and decayed leaf in Taiwan. A novel species is suggested to accommodate these isolates, for which the name C. xylosilytica sp. nov. is proposed. The type strain of C. xylosilytica sp. nov. is NRRL YB-2097(T) (=CBS 13984(T)=UFMG-CM-Y347(T)) and the allotype is UFMG-CM-Y409 (=CBS 14083). The novel species is heterothallic and complementary mating types are represented by the type and allotype strains. The MycoBank number is MB 811428. C1 [Cadete, Raquel M.; Cheab, Monaliza A. M.; Santos, Renata O.; Safar, Silvana V. B.; Rosa, Carlos A.] Univ Fed Minas Gerais, ICB, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. [Zilli, Jerri E.] Embrapa Agrobiol, Seropedica, RJ, Brazil. [Vital, Marcos J. S.] Univ Fed Roraima, Dept Biol, Boa Vista, Brazil. [Basso, Luiz C.] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Piracicaba, SP, Brazil. [Lee, Ching-Fu] Natl Hsinchu Univ Educ, Dept Appl Sci, Hsinchu 300, Taiwan. [Kurtzman, Cletus P.] ARS, USDA, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL USA. [Lachance, Marc-Andre] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada. RP Rosa, CA (reprint author), Univ Fed Minas Gerais, ICB, Dept Microbiol, CP 486, BR-31270901 Belo Horizonte, MG, Brazil. EM carlrosa@icb.ufmg.br FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [560715/2010-2]; Fundacao do Amparo a Pesquisa do Estado de Minas Gerais [APQ-01347-12, CBB - RDP-00094-10]; Financiadora de Estudos e Projetos [2084/07]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [2280/2008]; Ministry of Science and Technology, Taiwan [NSC 100-2621-B-134-001]; Natural Science and Engineering Research Council of Canada FX This work was funded by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (process 560715/2010-2), Fundacao do Amparo a Pesquisa do Estado de Minas Gerais (APQ-01347-12 and CBB - RDP-00094-10), the Financiadora de Estudos e Projetos (process 2084/07), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Procad-NF, process 2280/2008), the Ministry of Science and Technology, Taiwan (NSC 100-2621-B-134-001) and the Natural Science and Engineering Research Council of Canada (M. A. L.). The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 21 TC 2 Z9 2 U1 4 U2 6 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD SEP PY 2015 VL 65 BP 2968 EP 2974 DI 10.1099/ijs.0.000363 PN 9 PG 7 WC Microbiology SC Microbiology GA CV2SV UT WOS:000364108400030 PM 26025941 ER PT J AU Obadan, AO Kimble, BJ Rajao, D Lager, K Santos, JJS Vincent, A Perez, DR AF Obadan, Adebimpe O. Kimble, Brian J. Rajao, Daniela Lager, Kelly Santos, Jefferson J. S. Vincent, Amy Perez, Daniel R. TI Replication and transmission of mammalian-adapted H9 subtype influenza virus in pigs and quail SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID SIALIC-ACID RECEPTORS; TO-HUMAN TRANSMISSION; PANDEMIC H1N1 2009; AVIAN INFLUENZA; A VIRUSES; HUMAN INFECTION; HONG-KONG; GENETIC-CHARACTERIZATION; AIRBORNE TRANSMISSION; INTERNAL GENES AB Influenza A virus is a major pathogen of birds, swine and humans. Strains can jump between species in a process often requiring mutations and reassortment, resulting in outbreaks and, potentially, pandemics. H9N2 avian influenza is predominant in poultry across Asia and occasionally infects humans and swine. Pandemic H1N1 (H1N1 pdm) is endemic in humans and swine and has a history of reassortnnent in pigs. Previous studies have shown the compatibility of H9N2 and H1N1 pdm for reassortment in ferrets, a model for human infection and transmission. Here, the effects of ferret adaptation of H9 surface gene segments on the infectivity and transmission in at-risk natural hosts, specifically swine and quail, were analysed. Reassortant H9N1 and H9N2 viruses, carrying seven or six gene segments from H1N1 pdm, showed infectivity and transmissibility in swine, unlike the wholly avian H9N2 virus with ferret-adapted surface genes. In quail, only the reassortant H9N2 with the six internal gene segments from the H1N1 pdm strain was able to infect and transmit, although less efficiently than the wholly avian H9N2 virus with ferret-adapted surface genes. These results highlight that ferret-adapted mutations on the haemagglutinin of H9 subtype virus do not restrict the ability of the virus to infect swine and quail, and that the ability to transmit in these species depends on the context of the whole virus. As such, this study emphasizes the threat that H9N2 reassortant viruses pose to humans and agricultural species and the importance of the genetic constellation of the virus to its ability to replicate and transmit in natural hosts of influenza. C1 [Obadan, Adebimpe O.; Kimble, Brian J.; Santos, Jefferson J. S.; Perez, Daniel R.] Univ Maryland, Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA. [Rajao, Daniela; Lager, Kelly; Vincent, Amy] ARS, Virus & Prion Dis Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Perez, DR (reprint author), Univ Georgia, Dept Populat Hlth, Poultry Diagnost & Res Ctr, 953 Coll Stn Rd, Athens, GA 30602 USA. EM dperez1@uga.edu OI de Souza Rajao, Daniela/0000-0002-0772-0065 FU NIAID-NIH [HHSN266200700010C, HHSN272201400008C]; USDA-ARS FX We thank Diego Lopez and Haichen Song for technical assistance. This research was funded by NIAID-NIH contracts (HHSN266200700010C and HHSN272201400008C) and USDA-ARS. The studies presented above were performed prior to the USG moratorium on gain-of-function studies implemented on 17 October 2014. NR 72 TC 0 Z9 0 U1 1 U2 5 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD SEP PY 2015 VL 96 BP 2511 EP 2521 DI 10.1099/vir.0.000190 PN 9 PG 11 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CV0CQ UT WOS:000363915400004 PM 25986634 ER PT J AU Sun, HL Cunningham, FL Harris, J Xu, YF Long, LP Hanson-Dorr, K Baroch, JA Fioranelli, P Lutman, MW Li, T Pedersen, K Schmit, BS Cooley, J Lin, XX Jarman, RG DeLiberto, TJ Wan, XF AF Sun, Hailiang Cunningham, Fred L. Harris, Jillian Xu, Yifei Long, Li-Ping Hanson-Dorr, Katie Baroch, John A. Fioranelli, Paul Lutman, Mark W. Li, Tao Pedersen, Kerri Schmit, Brandon S. Cooley, Jim Lin, Xiaoxu Jarman, Richard G. DeLiberto, Thomas J. Wan, Xiu-Feng TI Dynamics of virus shedding and antibody responses in influenza A virus-infected feral swine SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID VIRAL-RNA REPLICATION; UNITED-STATES; PACKAGING SIGNALS; PIGS; H5N1; TRANSMISSION; MICE; PATHOGENICITY; CHINA; AMPLIFICATION AB Given their free-ranging habits, feral swine could serve as reservoirs or spatially dynamic 'mixing vessels' for influenza A virus (IAV). To better understand virus shedding patterns and antibody response dynamics in the context of IAV surveillance amongst feral swine, we used IAV of feral swine origin to perform infection experiments. The virus was highly infectious and transmissible in feral swine, and virus shedding patterns and antibody response dynamics were similar to those in domestic swine. In the virus-inoculated and sentinel groups, virus shedding lasted <= 6 and <= 9 days, respectively. Antibody titres in inoculated swine peaked at 1 : 840 on day 11 post-inoculation (p.i.), remained there until 21 days p.i. and dropped to <1 : 220 at 42 days p.i. Genomic sequencing identified changes in wildtype (WT) viruses and isolates from sentinel swine, most notably an amino acid divergence in nucleoprotein position 473. Using data from cell culture as a benchmark, sensitivity and specificity of a matrix gene-based quantitative reverse transcription-PCR method using nasal swab samples for detection of IAV in feral swine were 78.9 and 78.1 %, respectively. Using data from haemagglutination inhibition assays as a benchmark, sensitivity and specificity of an ELISA for detection of IAV-specific antibody were 95.4 and 95.0 %, respectively. Serological surveillance from 2009 to 2014 showed that similar to 7.58 % of feral swine in the USA were positive for IAV. Our findings confirm the susceptibility of IAV infection and the high transmission ability of IAV amongst feral swine, and also suggest the need for continued surveillance of IAVs in feral swine populations. C1 [Sun, Hailiang; Harris, Jillian; Xu, Yifei; Long, Li-Ping; Wan, Xiu-Feng] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Cunningham, Fred L.; Hanson-Dorr, Katie; Fioranelli, Paul] Anim & Plant Hlth Inspect Serv, Mississippi Field Stn, Natl Wildlife Res Ctr, Wildlife Serv,USDA, Mississippi State, MS USA. [Baroch, John A.; Schmit, Brandon S.; DeLiberto, Thomas J.] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Wildlife Serv, USDA, Ft Collins, CO USA. [Lutman, Mark W.; Pedersen, Kerri] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Ft Collins, CO USA. [Li, Tao; Lin, Xiaoxu; Jarman, Richard G.] Walter Reed Army Inst Res, Viral Dis Branch, Silver Spring, MD USA. [Cooley, Jim] Mississippi State Univ, Coll Vet Med, Dept Pathobiol & Populat Med, Mississippi State, MS 39762 USA. RP Wan, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM wan@cvm.msstate.edu OI Hanson-Dorr, Katie/0000-0003-4559-938X FU Wildlife Services, National Wildlife Research Center, US Department of Agriculture [13-7428-0961-CA, 14-7428-1041-CA]; National Institutes of Health [P20GM103646]; Global Emerging Infection Systems, a Division of the Armed Forces Health Surveillance Center FX This work was supported by the Wildlife Services, National Wildlife Research Center, US Department of Agriculture (13-7428-0961-CA and 14-7428-1041-CA), and partially funded by the National Institutes of Health (P20GM103646). Funding for virus sequencing was supported by the Global Emerging Infection Systems, a Division of the Armed Forces Health Surveillance Center. The opinions or assertions contained herein are the private views of the authors and are not to be construed as reflecting the official views of the US Army or the US Department of Defense. NR 56 TC 2 Z9 2 U1 0 U2 2 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD SEP PY 2015 VL 96 BP 2569 EP 2578 DI 10.1099/jgv.0.000225 PN 9 PG 10 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CV0CQ UT WOS:000363915400009 PM 26297148 ER PT J AU Gopalakrishnan, R Thomas, VA Coulston, JW Wynne, RH AF Gopalakrishnan, Ranjith Thomas, Valerie A. Coulston, John W. Wynne, Randolph H. TI Prediction of Canopy Heights over a Large Region Using Heterogeneous Lidar Datasets: Efficacy and Challenges SO REMOTE SENSING LA English DT Article DE forest inventory; forestry; forest mensuration; lidar; canopy heights; wall-to-wall mapping; co-registration; FIA ID CONTERMINOUS UNITED-STATES; DISCRETE RETURN LIDAR; FOREST INVENTORY; AIRBORNE LIDAR; LAND-COVER; REAL-TIME; GPS; RESOURCES; ACCURACY; PLOTS AB Generating accurate and unbiased wall-to-wall canopy height maps from airborne lidar data for large regions is useful to forest scientists and natural resource managers. However, mapping large areas often involves using lidar data from different projects, with varying acquisition parameters. In this work, we address the important question of whether one can accurately model canopy heights over large areas of the Southeastern US using a very heterogeneous dataset of small-footprint, discrete-return airborne lidar data (with 76 separate lidar projects). A unique aspect of this effort is the use of nationally uniform and extensive field data (similar to 1800 forested plots) from the Forest Inventory and Analysis (FIA) program of the US Forest Service. Preliminary results are quite promising: Over all lidar projects, we observe a good correlation between the 85th percentile of lidar heights and field-measured height (r = 0.85). We construct a linear regression model to predict subplot-level dominant tree heights from distributional lidar metrics (R-2 = 0.74, RMSE = 3.0 m, n = 1755). We also identify and quantify the importance of several factors (like heterogeneity of vegetation, point density, the predominance of hardwoods or softwoods, the average height of the forest stand, slope of the plot, and average scan angle of lidar acquisition) that influence the efficacy of predicting canopy heights from lidar data. For example, a subset of plots (coefficient of variation of vegetation heights <0.2) significantly reduces the RMSE of our model from 3.0-2.4 m (similar to 20% reduction). We conclude that when all these elements are factored into consideration, combining data from disparate lidar projects does not preclude robust estimation of canopy heights. C1 [Gopalakrishnan, Ranjith; Thomas, Valerie A.; Wynne, Randolph H.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Coulston, John W.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN 37919 USA. RP Gopalakrishnan, R (reprint author), Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. EM ranjith7@vt.edu; thomasv@vt.edu; jcoulston@fs.fed.us; wynne@vt.edu FU PINEMAP project - USDA's National Institute of Food and Agriculture (NIFA); United States Department of Agriculture (USDA) McIntire-Stennis Formula Grant FX This work was funded by the PINEMAP project (pinemap.org) sponsored by the USDA's National Institute of Food and Agriculture (NIFA). We would also like to acknowledge the United States Department of Agriculture (USDA) McIntire-Stennis Formula Grant. NR 52 TC 0 Z9 0 U1 3 U2 9 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD SEP PY 2015 VL 7 IS 9 BP 11036 EP 11060 DI 10.3390/rs70911036 PG 25 WC Remote Sensing SC Remote Sensing GA CT0UA UT WOS:000362511400002 ER PT J AU Voyles, J Kilpatrick, AM Collins, JP Fisher, MC Frick, WF McCallum, H Willis, CKR Blehert, DS Murray, KA Puschendorf, R Rosenblum, EB Bolker, BM Cheng, TL Langwig, KE Lindner, DL Toothman, M Wilber, MQ Briggs, CJ AF Voyles, Jamie Kilpatrick, A. Marm Collins, James P. Fisher, Matthew C. Frick, Winifred F. McCallum, Hamish Willis, Craig K. R. Blehert, David S. Murray, Kris A. Puschendorf, Robert Rosenblum, Erica Bree Bolker, Benjamin M. Cheng, Tina L. Langwig, Kate E. Lindner, Daniel L. Toothman, Mary Wilber, Mark Q. Briggs, Cheryl J. TI Moving Beyond Too Little, Too Late: Managing Emerging Infectious Diseases in Wild Populations Requires International Policy and Partnerships SO ECOHEALTH LA English DT Editorial Material ID WHITE-NOSE SYNDROME; CHYTRIDIOMYCOSIS; DESTRUCTANS; GEOMYCES; PATHOGEN; DECLINES; AMERICA; HEALTH C1 [Voyles, Jamie] New Mexico Inst Min & Technol, Dept Biol, Socorro, NM 87801 USA. [Kilpatrick, A. Marm; Frick, Winifred F.; Cheng, Tina L.; Langwig, Kate E.] Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. [Collins, James P.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Fisher, Matthew C.] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, London, England. [McCallum, Hamish] Griffith Univ, Sch Environm, Nathan, Qld 4111, Australia. [Willis, Craig K. R.] Univ Winnipeg, Dept Biol, Winnipeg, MB R3B 2E9, Canada. [Blehert, David S.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Murray, Kris A.] EcoHlth Alliance, New York, NY USA. [Puschendorf, Robert] Univ Plymouth, Sch Biol Sci, Plymouth PL4 8AA, Devon, England. [Rosenblum, Erica Bree] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Bolker, Benjamin M.] McMaster Univ, Dept Math & Stat, Hamilton, ON, Canada. [Bolker, Benjamin M.] McMaster Univ, Dept Biol, Hamilton, ON, Canada. [Lindner, Daniel L.] US Forest Serv, Ctr Mycol Res, Madison, WI USA. [Toothman, Mary; Wilber, Mark Q.; Briggs, Cheryl J.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. RP Voyles, J (reprint author), New Mexico Inst Min & Technol, Dept Biol, Socorro, NM 87801 USA. EM jamie.voyles@gmail.com RI McCallum, Hamish/E-1638-2013; OI McCallum, Hamish/0000-0002-3493-0412; Fisher, Matthew/0000-0002-1862-6402 NR 17 TC 7 Z9 7 U1 4 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD SEP PY 2015 VL 12 IS 3 BP 404 EP 407 DI 10.1007/s10393-014-0980-5 PG 4 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CU7MH UT WOS:000363723300003 PM 25287279 ER PT J AU Fagre, AC Patyk, KA Nol, P Atwood, T Hueffer, K Duncan, C AF Fagre, Anna C. Patyk, Kelly A. Nol, Pauline Atwood, Todd Hueffer, Karsten Duncan, Colleen TI A Review of Infectious Agents in Polar Bears (Ursus maritimus) and Their Long-Term Ecological Relevance SO ECOHEALTH LA English DT Review DE bacteria; disease; fungi; infection; parasites; polar bear; Ursus maritimus; virus; vital rates ID SOUTHERN BEAUFORT SEA; RECENT CLIMATE-CHANGE; BAYLISASCARIS-TRANSFUGA; TOXOPLASMA-GONDII; SEROLOGIC SURVEY; NORTHERN ALASKA; WILDLIFE HEALTH; MARINE MAMMALS; BARENTS SEA; ZOO ANIMALS AB Disease was a listing criterion for the polar bear (Ursus maritimus) as threatened under the Endangered Species Act in 2008; it is therefore important to evaluate the current state of knowledge and identify any information gaps pertaining to diseases in polar bears. We conducted a systematic literature review focused on infectious agents and associated health impacts identified in polar bears. Overall, the majority of reports in free-ranging bears concerned serosurveys or fecal examinations with little to no information on associated health effects. In contrast, most reports documenting illness or pathology referenced captive animals and diseases caused by etiologic agents not representative of exposure opportunities in wild bears. As such, most of the available infectious disease literature has limited utility as a basis for development of future health assessment and management plans. Given that ecological change is a considerable risk facing polar bear populations, future work should focus on cumulative effects of multiple stressors that could impact polar bear population dynamics. C1 [Fagre, Anna C.; Duncan, Colleen] Colorado State Univ, Coll Vet Med & Biomed Sci, Vet Diagnost Lab, Ft Collins, CO 80524 USA. [Patyk, Kelly A.] APHIS, Ctr Epidemiol & Anim Hlth, STAS, VS,USDA, Ft Collins, CO 80526 USA. [Nol, Pauline] APHIS, Wildlife Livestock Dis Invest Team, STAS, VS,USDA, Ft Collins, CO 80521 USA. [Atwood, Todd] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Hueffer, Karsten] Univ Alaska Fairbanks, Coll Nat Sci & Math, Dept Vet Med, Fairbanks, AK 99775 USA. RP Duncan, C (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Vet Diagnost Lab, 300 West Drake Rd, Ft Collins, CO 80524 USA. EM colleen.duncan@colostate.edu NR 101 TC 1 Z9 2 U1 23 U2 55 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD SEP PY 2015 VL 12 IS 3 BP 528 EP 539 DI 10.1007/s10393-015-1023-6 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CU7MH UT WOS:000363723300018 PM 25791679 ER PT J AU Fagre, AC Patyk, KA Nol, P Atwood, T Hueffer, K Duncan, C AF Fagre, Anna C. Patyk, Kelly A. Nol, Pauline Atwood, Todd Hueffer, Karsten Duncan, Colleen TI A Review of Infectious Agents in Polar Bears (Ursus maritimus) and Their Long-Term Ecological Relevance (vol 12, pg 528, 2015) SO ECOHEALTH LA English DT Correction C1 [Fagre, Anna C.; Duncan, Colleen] Colorado State Univ, Coll Vet Med & Biomed Sci, Vet Diagnost Lab, Ft Collins, CO 80524 USA. [Patyk, Kelly A.] APHIS, Ctr Epidemiol & Anim Hlth, STAS, VS,USDA, Ft Collins, CO 80526 USA. [Nol, Pauline] APHIS, Wildlife Livestock Dis Invest Team, STAS, VS,USDA, Ft Collins, CO 80521 USA. [Atwood, Todd] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Hueffer, Karsten] Univ Alaska Fairbanks, Coll Nat Sci & Math, Dept Vet Med, Fairbanks, AK 99775 USA. RP Duncan, C (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Vet Diagnost Lab, 300 West Drake Rd, Ft Collins, CO 80524 USA. EM colleen.duncan@colostate.edu NR 1 TC 0 Z9 0 U1 2 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD SEP PY 2015 VL 12 IS 3 BP 540 EP 540 DI 10.1007/s10393-015-1070-z PG 1 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CU7MH UT WOS:000363723300019 PM 26446003 ER PT J AU Yang, J Tian, HQ Tao, B Ren, W Lu, CQ Pan, SF Wang, YH Liu, YQ AF Yang, Jia Tian, Hanqin Tao, Bo Ren, Wei Lu, Chaoqun Pan, Shufen Wang, Yuhang Liu, Yongqiang TI Century-scale patterns and trends of global pyrogenic carbon emissions and fire influences on terrestrial carbon balance SO GLOBAL BIOGEOCHEMICAL CYCLES LA English DT Article DE biomass burning; tree mortality; net primary productivity; pyrogenic carbon emissions; terrestrial carbon sink ID NET PRIMARY PRODUCTION; NORMALIZED BURN RATIO; BOREAL FOREST; UNITED-STATES; ECOSYSTEM CARBON; ENVIRONMENTAL-CHANGES; PRIMARY PRODUCTIVITY; NITROGEN DEPOSITION; DROUGHT SENSITIVITY; VEGETATION MODEL AB Fires have consumed a large amount of terrestrial organic carbon and significantly influenced terrestrial ecosystems and the physical climate system over the past century. Although biomass burning has been widely investigated at a global level in recent decades via satellite observations, less work has been conducted to examine the century-scale changes in global fire regimes and fire influences on the terrestrial carbon balance. In this study, we investigated global pyrogenic carbon emissions and fire influences on the terrestrial carbon fluxes from 1901 to 2010 by using a process-based land ecosystem model. Our results show a significant declining trend in global pyrogenic carbon emissions between the early 20th century and the mid-1980s but a significant upward trend between the mid-1980s and the 2000s as a result of more frequent fires in ecosystems with high carbon storage, such as peatlands and tropical forests. Over the past 110years, average pyrogenic carbon emissions were estimated to be 2.43PgCyr(-1) (1Pg=10(15)g), and global average combustion rate (defined as carbon emissions per unit area burned) was 537.85gCm(-2) burned area. Due to the impacts of fires, the net primary productivity and carbon sink of global terrestrial ecosystems were reduced by 4.14PgCyr(-1) and 0.57PgCyr(-1), respectively. Our study suggests that special attention should be paid to fire activities in the peatlands and tropical forests in the future. Practical management strategies, such as minimizing forest logging and reducing the rate of cropland expansion in the humid regions, are in need to reduce fire risk and mitigate fire-induced greenhouse gases emissions. C1 [Yang, Jia; Tian, Hanqin; Tao, Bo; Ren, Wei; Lu, Chaoqun; Pan, Shufen] Auburn Univ, Sch Forestry & Wildlife Sci, Int Ctr Climate & Global Change Res, Auburn, AL 36849 USA. [Wang, Yuhang] Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Athens, GA USA. RP Tian, HQ (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, Int Ctr Climate & Global Change Res, Auburn, AL 36849 USA. EM tianhan@auburn.edu RI Wang, Yuhang/B-5578-2014; Ren, Wei/G-8317-2016; Ren, Wei/I-4048-2014; Yang, Jia/A-6483-2012; OI Ren, Wei/0000-0002-4840-4835; Yang, Jia/0000-0003-2019-9603; Tian, Hanqin/0000-0002-1806-4091 FU NSF/USDA/DOE Decadal and Regional Climate Prediction using Earth System Models [NSF1243232, NSF1243220, NIFC2013-35100-20516]; USDA/USDI Joint Fire Science Program [JFSP 11172]; NSF Dynamics of Coupled Natural and Human Systems [1210360] FX This work has been supported by NSF/USDA/DOE Decadal and Regional Climate Prediction using Earth System Models (NSF1243232, NSF1243220, and NIFC2013-35100-20516), USDA/USDI Joint Fire Science Program (JFSP 11172), and NSF Dynamics of Coupled Natural and Human Systems (1210360). We thank the GFED developing group (http://www.globalfiredata.org/) for their provision of global satellite-based fire products. We appreciate the comments from Shree Dangal and contributions of other DLEM developers. We also thank the anonymous reviewers for their valuable comments. The availability of model driving forces and benchmark data is described in the supporting information. NR 107 TC 3 Z9 3 U1 4 U2 23 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0886-6236 EI 1944-9224 J9 GLOBAL BIOGEOCHEM CY JI Glob. Biogeochem. Cycle PD SEP PY 2015 VL 29 IS 9 BP 1549 EP 1566 DI 10.1002/2015GB005160 PG 18 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA CU7GD UT WOS:000363703800013 ER PT J AU Jimenez, LR Hall, WA Rodriquez, MS Cooper, WJ Muhareb, J Jones, T Walse, SS AF Jimenez, Leonel R. Hall, Wiley A. Rodriquez, Matthew S. Cooper, William J. Muhareb, Jeanette Jones, Tom Walse, Spencer S. TI Quantifying Residues from Postharvest Propylene Oxide Fumigation of Almonds and Walnuts SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID GAS-CHROMATOGRAPHIC DETERMINATION; METHYL-BROMIDE AB A novel analytical approach involving solvent extraction with methyl tert-butyl ether (MTBE) followed by GC was developed to quantify residues that result from the postharvest fumigation of almonds and walnuts with propylene oxide (PPO). Verification and quantification of PPO, propylene chlorohydrin (PCH) [1-chloropropan-2-ol (PCH-1) and 2-chloropropan-1-ol (PCH-2)], and propylene bromohydrin (PBH) [1-bromopropan-2-ol (PBH-1) and 2-bromopropan-1-ol (PBH-2)] was accomplished with a combination of electron impact ionization MS (EIMS), negative ion chemical ionization MS (NCIMS), and electron capture detection (ECD). Respective GC/EIMS LOQs for PPO, PCH-1, PCH-2, PBH-1, and PBH-2 in MTBE extracts were [ppm (mu g/g nut)] 0.9, 2.1, 2.5, 30.3, and 50.0 for almonds and 0.8, 2.2, 2.02, 41.6, and 45.7 for walnuts. Relative to GC/EIMS, GC-ECD analyses resulted in no detection of PPO, similar detector responses for PCH isomers, and >100-fold more sensitive detection of PBH isomers. NCIMS did not enhance detection of PBH isomers relative to EIMS and was, respectively, approximately 20-, 5-, and 10-fold less sensitive to PPO, PCH-1, and PCH-2. MTBE extraction efficiencies were >90% for all analytes. The 10-fold concentration of MTBE extracts yielded recoveries of 85-105% for the PBH isomers and a concomitant decrease in LODs and LOQs across detector types. The recoveries of PCH isomers and PRO in the MTBE concentrate were relatively low (approximately 50 to 75%), which confound improvements in LODs and LOQs regardless of detector type. C1 [Jimenez, Leonel R.; Rodriquez, Matthew S.; Cooper, William J.] Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA 92697 USA. [Hall, Wiley A.; Walse, Spencer S.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Muhareb, Jeanette; Jones, Tom] Dried Fruit & Nut Assoc Calif, Fresno, CA 93705 USA. RP Walse, SS (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM spencer.walse@ars.usda.gov FU USDA-Agricultural Research Service; Almond Board of California; California Walnut Board FX This research was funded by the USDA-Agricultural Research Service, the Almond Board of California, and the California Walnut Board. NR 11 TC 0 Z9 0 U1 2 U2 9 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD SEP-OCT PY 2015 VL 98 IS 5 BP 1423 EP 1427 DI 10.5740/jaoacint.14-199 PG 5 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA CU5WF UT WOS:000363602000032 PM 26525262 ER PT J AU Wolfe, JD Johnson, EI AF Wolfe, Jared D. Johnson, Erik I. TI Geolocator reveals migratory and winter movements of a Prothonotary Warbler SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE intratropical migration; overwinter movement; Protonotaria citrea; stopover; trans-Gulf ID LIGHT-LEVEL GEOLOCATORS; MIGRANT BIRDS; COSTA-RICA; CONNECTIVITY; ABUNDANCE; MANGROVE; TRACKING; AMERICA; SITES; AREAS AB Prothonotary Warblers (Protonotaria citrea) are Nearctic-Neotropic migrants that have experienced declining populations over the past 50 yr. Determining their migration routes and wintering areas are critical steps in identifying habitats used by species and populations of conservation concern. We captured a male Prothonotary Warbler on its breeding territory in Louisiana in June 2013 and attached a geolocator. We recaptured this male in March 2014 and analysis of the geolocator data revealed that the male traveled an estimated 7950 km through seven countries. Fall migration was characterized by movements south into Central America on 13 August 2014, then east to the Greater Antilles for about 1 mo, followed by a second movement south to Panama or Colombia on 15 November 2014 where the male remained for the rest of the winter. Spring migration occurred rapidly, with the bird leaving its southern wintering area on 4 March 2014 and returning to its breeding territory on 23 March 2014. The movements of this male Prothonotary Warbler add to accumulating evidence that many migrant birds exhibit either prolonged stopover behavior or secondary migratory movements. Factors contributing to prolonged stopover behavior or additional migratory movements of the Prothonotary Warbler in our study warrant further investigation. C1 [Wolfe, Jared D.; Johnson, Erik I.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.; Johnson, Erik I.] Baton Rouge Audubon Soc, Louisiana Bird Observ, Baton Rouge, LA 70896 USA. [Wolfe, Jared D.] USDA Forest Serv, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA 95521 USA. [Wolfe, Jared D.] Klamath Bird Observ, Ashland, OR 97520 USA. [Johnson, Erik I.] Natl Audubon Soc, Audubon Louisiana, Baton Rouge, LA 70808 USA. RP Wolfe, JD (reprint author), Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, 227 RNR Bldg, Baton Rouge, LA 70803 USA. EM jdw@klamathbird.org FU Parks and Recreation Commission for the Parish of East Baton Rouge (BREC); Louisiana State University; Baton Rouge Audubon Society; Louisiana Environment and Education Council (LEEC) FX This project would not have been possible without the dedicated volunteers of the Louisiana Bird Observatory. In particular, we thank E. Liffmann for designing, building, and deploying numerous Prothonotary Warbler nest boxes. Additionally, J. E. Hartgerink, J. Seagle, J. Newell, and C. Newell were critically important in deploying, maintaining, and monitoring nest boxes as well as observing individually marked Prothonotary Warblers. Louisiana Bird Observatory volunteer banders were equally critical in initially banding and monitoring Prothonotary Warblers at Bluebonnet, in particular D. Mooney, L. Powell, K. Brzeski, S. Duke-Sylvester, B. Karmacharya, S. Borchert, M. Brady, S. Lee, C. Johnson, M. Arias, A. Anderson, C. Rutt, and S. Wheeler. Thanks to the Parks and Recreation Commission for the Parish of East Baton Rouge (BREC) and Louisiana State University for their continual support. J. V. Remsen provided the nest box design idea and specs. This project would not have been possible without the steadfast emotional and financial support of Baton Rouge Audubon Society. Additional support was provided by a Louisiana Environment and Education Council (LEEC) research grant. Our manuscript was greatly improved by comments from G. Ritchison and two anonymous reviewers. This manuscript was approved for publication by the Director of the Louisiana Agricultural Experimental Station as manuscript no. 2015-241-22489. This is contribution number 7 of the Louisiana Bird Observatory. NR 37 TC 4 Z9 4 U1 8 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 EI 1557-9263 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD SEP PY 2015 VL 86 IS 3 BP 238 EP 243 DI 10.1111/jofo.12107 PG 6 WC Ornithology SC Zoology GA CU9RL UT WOS:000363882500005 ER PT J AU Trabelsi, S AF Trabelsi, Samir TI Variation of the dielectric properties of chicken meat with frequency and temperature SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE Dielectric properties; Chicken meat; Frequency dependence; Temperature dependence; Cole-Cole plot ID MOISTURE-CONTENT; BULK-DENSITY; WATER; SPECTRA AB Dielectric properties of chicken breast meat were measured with an open-ended coaxial-line probe between 200 MHz and 20 GHz at temperatures ranging from -20 to +25 degrees C. To ensure temperature uniformity between the different components of the measurement assembly, the measurements were performed in a temperature-controlled chamber. At a given temperature, the frequency dependence of the dielectric constant reveals two relaxations while those of the dielectric loss factor are dominated by ionic conduction in the lower range and a broad dipolar relaxation at higher frequencies. At a given frequency, the temperature dependence reveals a sharp increase of dielectric properties at about 0 degrees C which is typical of materials with high water content and indicates the transition from ice-like behavior to liquid-like behavior. 3-D representations of the dielectric properties as a function of frequency and temperature show the combined influence of water binding, ionic conduction, and dipolar relaxation. C1 ARS, Qual & Safety Assessment Res Unit, USDA, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. RP Trabelsi, S (reprint author), ARS, Qual & Safety Assessment Res Unit, USDA, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. EM samir.trabelsi@ars.usda.gov NR 23 TC 0 Z9 0 U1 6 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD SEP PY 2015 VL 9 IS 3 BP 299 EP 304 DI 10.1007/s11694-015-9235-6 PG 6 WC Food Science & Technology SC Food Science & Technology GA CU6XT UT WOS:000363679300007 ER PT J AU Smith, L Beck, JJ AF Smith, Lincoln Beck, John J. TI Duration of emission of volatile organic compounds from mechanically damaged plant leaves SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Biological control; Volatile organic compounds; Mechanical damage; Host plant specificity; Sesquiterpene ID BIOLOGICAL-CONTROL AGENT; SUBTRIBE CENTAUREINAE ASTERACEAE; HOST-RANGE; CERATAPION-BASICORNE; HERBIVOROUS INSECTS; PARASITIC WASPS; COTTON PLANTS; RESPONSES; WEEDS; SPECIFICITY AB Classical biological control of invasive alien weeds depends on the use of arthropod herbivores that are sufficiently host specific to avoid risk of injuring nontarget plants. Host plant specificity is usually evaluated by using a combination of behavioral and developmental experiments under choice, no-choice and field conditions. Secondary plant compounds are likely to have an important influence on host plant specificity. However, relatively little is known about the volatile organic compounds (VOCs) that are emitted by target and nontarget plants, and how environmental conditions may affect their emission. Previous studies have shown that mechanical damage of leaves increases the composition and content of VOCs emitted. In this study we measured the VOC emissions of five species of plants in the subtribe Centaureinae (Asteraceae) - Carthamus tinctorius, Centaurea cineraria, Centaurea melitensis, Centaurea rothrockii, and Centaurea solstitialis - that have previously been used in host specificity experiments for a prospective biological control agent of yellow starthistle (C. solstitialis). Leaves of each plant were punctured with a needle and the VOCs were collected by solid-phase microextraction (SPME) periodically over 48 h and analyzed by GC-MS. A total of 49 compounds were detected. Damage caused an immediate increase of 200-600% in the composition of VOCs emitted from each plant species, and the amounts generally remained high for at least 48 h. The results indicate that a very unspecific mechanical damage can cause a prolonged change in the VOC profile of plants. Published by Elsevier GmbH. C1 [Smith, Lincoln; Beck, John J.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Smith, L (reprint author), USDA ARS, European Biol Control Lab, 810 Ave,Campus Agropolis, F-34980 Montferrier Sur Lez, France. EM Link.smith@ars.usda.gov NR 59 TC 0 Z9 0 U1 2 U2 21 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD SEP 1 PY 2015 VL 188 BP 19 EP 28 DI 10.1016/j.jplph.2015.08.003 PG 10 WC Plant Sciences SC Plant Sciences GA CU4EL UT WOS:000363479600003 PM 26398629 ER PT J AU Ravussin, E Redman, LM Rochon, J Das, SK Fontana, L Kraus, WE Romashkan, S Williamson, DA Meydani, SN Villareal, DT Smith, SR Stein, RI Scott, TM Stewart, TM Saltzman, E Klein, S Bhapkar, M Martin, CK Gilhooly, CH Holloszy, JO Hadley, EC Roberts, SB AF Ravussin, Eric Redman, Leanne M. Rochon, James Das, Sai Krupa Fontana, Luigi Kraus, William E. Romashkan, Sergei Williamson, Donald A. Meydani, Simin N. Villareal, Dennis T. Smith, Steven R. Stein, Richard I. Scott, Tammy M. Stewart, Tiffany M. Saltzman, Edward Klein, Samuel Bhapkar, Manju Martin, Corby K. Gilhooly, Cheryl H. Holloszy, John O. Hadley, Evan C. Roberts, Susan B. CA CALERIE Study Grp TI A 2-Year Randomized Controlled Trial of Human Caloric Restriction: Feasibility and Effects on Predictors of Health Span and Longevity SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE Metabolism; Nutrition; Risk factors; Biomarkers; Caloric restriction ID RHESUS-MONKEYS; COMPREHENSIVE ASSESSMENT; ENERGY-EXPENDITURE; REDUCING INTAKE; MORTALITY; CALERIE; EXERCISE; BIOMARKERS; INTERVENTIONS; TEMPERATURE AB Background. Caloric restriction (CR), energy intake reduced below ad libitum (AL) intake, increases life span in many species. The implications for humans can be clarified by randomized controlled trials of CR. Methods. To determine CR's feasibility, safety, and effects on predictors of longevity, disease risk factors, and quality of life in nonobese humans aged 21-51 years, 218 persons were randomized to a 2-year intervention designed to achieve 25% CR or to AL diet. Outcomes were change from baseline resting metabolic rate adjusted for weight change ("RMR residual") and core temperature (primary); plasma triiodothyronine (T3) and tumor necrosis factor-alpha (secondary); and exploratory physiological and psychological measures. Results. Body mass index averaged 25.1 (range: 21.9-28.0 kg/m(2)). Eighty-two percent of CR and 95% of AL participants completed the protocol. The CR group achieved 11.7 +/- 0.7 % CR (mean +/- standard error) and maintained 10.4 +/- 0.4% weight loss. Weight change in AL was negligible. RMR residual decreased significantly more in CR than AL at 12 months (p =.04) but not 24 months (M24). Core temperature change differed little between groups. T3 decreased more in CR at M12 and M24 (p <.001), while tumor necrosis factor-alpha decreased significantly more only at M24 (p =.02). CR had larger decreases in cardiometabolic risk factors and in daily energy expenditure adjusted for weight change, without adverse effects on quality of life. Conclusions. Sustained CR is feasible in nonobese humans. The effects of the achieved CR on correlates of human survival and disease risk factors suggest potential benefits for aging-related outcomes that could be elucidated by further human studies. C1 [Ravussin, Eric; Redman, Leanne M.; Williamson, Donald A.; Smith, Steven R.; Stewart, Tiffany M.; Martin, Corby K.] Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Rochon, James; Kraus, William E.; Bhapkar, Manju] Duke Clin Res Inst, Durham, NC USA. [Rochon, James; Kraus, William E.; Bhapkar, Manju] Duke Univ, Sch Med, Durham, NC USA. [Rochon, James] Rho Fed Syst, Chapel Hill, NC USA. [Das, Sai Krupa; Meydani, Simin N.; Scott, Tammy M.; Saltzman, Edward; Gilhooly, Cheryl H.; Roberts, Susan B.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fontana, Luigi; Villareal, Dennis T.; Stein, Richard I.; Klein, Samuel; Holloszy, John O.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA. [Fontana, Luigi] Univ Brescia, Sch Med, Dept Clin & Expt Sci, I-25121 Brescia, Italy. [Fontana, Luigi] CEINGE Biotecnol Avanzate, Naples, Italy. [Romashkan, Sergei; Hadley, Evan C.] NIA, Bethesda, MD 20892 USA. [Smith, Steven R.] Florida Hosp, Translat Res Inst Metab & Diabet, Orlando, FL USA. [Smith, Steven R.] Sanford Burnham Med Res Inst, Orlando, FL USA. RP Hadley, EC (reprint author), NIA, Gateway Bldg,Suite 3C307, Bethesda, MD 20892 USA. EM ehadley@nih.gov RI John, Speakman/A-9494-2008; OI John, Speakman/0000-0002-2457-1823; Schram, Miranda/0000-0003-0515-4124 FU National Institute on Aging; National Institute of Diabetes and Digestive and Kidney Diseases; National Institutes of Health [U01AG022132, U01AG020478, U01AG020487, U01AG020480] FX This study was supported by the National Institute on Aging; National Institute of Diabetes and Digestive and Kidney Diseases; National Institutes of Health Cooperative Agreements (U01AG022132, U01AG020478, U01AG020487, and U01AG020480). NR 37 TC 33 Z9 33 U1 13 U2 28 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD SEP PY 2015 VL 70 IS 9 BP 1097 EP 1104 DI 10.1093/gerona/glv057 PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA CU4FK UT WOS:000363482300007 PM 26187233 ER PT J AU Oldfield, EE Felson, AJ Auyeung, DSN Crowther, TW Sonti, NF Harada, Y Maynard, DS Sokol, NW Ashton, MS Warren, RJ Hallett, RA Bradford, MA AF Oldfield, Emily E. Felson, Alexander J. Auyeung, D. S. Novem Crowther, Thomas W. Sonti, Nancy F. Harada, Yoshiki Maynard, Daniel S. Sokol, Noah W. Ashton, Mark S. Warren, Robert J., II Hallett, Richard A. Bradford, Mark A. TI Growing the urban forest: tree performance in response to biotic and abiotic land management SO RESTORATION ECOLOGY LA English DT Article DE afforestation; compost; ecosystem services; green infrastructure; native species; restoration; urban forestry ID GREEN INFRASTRUCTURE; ECOSYSTEM SERVICES; SOILS; RESTORATION; ECOLOGY; GROWTH; CITIES; ESTABLISHMENT; REGENERATION; PLANTATIONS AB Forests are vital components of the urban landscape because they provide ecosystem services such as carbon sequestration, storm-water mitigation, and air-quality improvement. To enhance these services, cities are investing in programs to create urban forests. A major unknown, however, is whether planted trees will grow into the mature, closed-canopied forest on which ecosystem service provision depends. We assessed the influence of biotic and abiotic land management on planted tree performance as part of urban forest restoration in New York City, U.S.A. Biotic treatments were designed to improve tree growth, with the expectation that higher tree species composition (six vs. two) and greater stand complexity (with shrubs vs. without) would facilitate tree performance. Similarly, the abiotic treatment (compost amendment vs. without) was expected to increase tree performance by improving soil conditions. Growth and survival was measured for approximately 1,300 native saplings across three growing seasons. The biotic and abiotic treatments significantly improved tree performance, where shrub presence increased tree height for five of the six tree species, and compost increased basal area and stem volume of all species. Species-specific responses, however, highlighted the difficulty of achieving rapid growth with limited mortality. Pioneer species had the highest growth in stem volume over 3 years (up to 3,500%), but also the highest mortality (up to 40%). Mid-successional species had lower mortality (<16%), but also the slowest growth in volume (approximately 500% in volume). Our results suggest that there will be trade-offs between optimizing tree growth versus survival when implementing urban tree planting initiatives. C1 [Oldfield, Emily E.; Felson, Alexander J.; Crowther, Thomas W.; Harada, Yoshiki; Maynard, Daniel S.; Sokol, Noah W.; Ashton, Mark S.; Bradford, Mark A.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Felson, Alexander J.] Yale Univ, Sch Architecture, New Haven, CT 06511 USA. [Auyeung, D. S. Novem] NYC Urban Field Stn, NYC Dept Parks & Recreat, Bayside, NY 11359 USA. [Sonti, Nancy F.] USDA Forest Serv, NYC Urban Field Stn, No Res Stn, Bayside, NY 11359 USA. [Sonti, Nancy F.] USDA Forest Serv, Baltimore Field Stn, No Res Stn, Baltimore, MD 21228 USA. [Harada, Yoshiki] Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. [Warren, Robert J., II] SUNY Coll Buffalo, Dept Biol, Buffalo, NY 14222 USA. [Hallett, Richard A.] USDA Forest Serv, No Res Stn, Durham, NH 03824 USA. RP Oldfield, EE (reprint author), Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. EM emily.oldfield@yale.edu RI Bradford, Mark/G-3850-2012; OI Bradford, Mark/0000-0002-2022-8331; Warren II, Robert/0000-0001-6765-2351; Auyeung, D. S. Novem/0000-0002-4038-3865 FU Global Institute of Sustainable Forestry; Yale School of Forestry and Environmental Studies FX This work was funded in part by the Global Institute of Sustainable Forestry and the Yale School of Forestry and Environmental Studies. We thank the New York City Department of Parks & Recreation for constructing the study site and facilitating the research and the New York City Urban Field Station for logistical support. NR 42 TC 2 Z9 2 U1 12 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD SEP PY 2015 VL 23 IS 5 BP 707 EP 718 DI 10.1111/rec.12230 PG 12 WC Ecology SC Environmental Sciences & Ecology GA CU6UT UT WOS:000363670400026 ER PT J AU Jaenicke, EC Carlson, AC AF Jaenicke, Edward C. Carlson, Andrea C. TI Estimating and Investigating Organic Premiums for Retail-Level Food Products SO AGRIBUSINESS LA English DT Article ID HEDONIC ANALYSIS; PRICES; ATTRIBUTES; MARKETS AB Using 7 years of household-level scanner data, we use a hedonic pricing model to estimate the organic price premium for four retail-level food products. In each case, we find strong organic premiums of about 30% for bagged carrots, over 40% for canned soup, over 50% for coffee, and over 70% for milk. Using the estimated results from the hedonic price model, we pool the results across products, markets, and years, and then estimate a novel second-stage model where we uncover retail-level market factors associated with higher or lower premiums. For example, we find that category-level organic sales are associated with higher organic price premiums, whereas category-level nonorganic sales are associated with lower premiums. Taken collectively, our results suggest that organic price premiums are not threatened in the near term, but that several competing factors help moderate the size of the premiums. [EconLit citations: Q130]. (C) 2015 Wiley Periodicals, Inc. C1 [Jaenicke, Edward C.] Penn State Univ, Agr Econ Sociol & Educ, University Pk, PA 16802 USA. [Carlson, Andrea C.] ERS, USDA, Washington, DC 20250 USA. RP Jaenicke, EC (reprint author), Penn State Univ, Agr Econ Sociol & Educ, 208C Armsby, University Pk, PA 16802 USA. EM tjaenicke@psu.edu; acarlson@ers.usda.gov NR 24 TC 0 Z9 0 U1 4 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0742-4477 EI 1520-6297 J9 AGRIBUSINESS JI Agribusiness PD FAL PY 2015 VL 31 IS 4 BP 453 EP 471 DI 10.1002/agr.21413 PG 19 WC Agricultural Economics & Policy; Economics; Food Science & Technology SC Agriculture; Business & Economics; Food Science & Technology GA CU1IM UT WOS:000363274300001 ER PT J AU Stewart, H Hyman, J Dong, DS AF Stewart, Hayden Hyman, Jeffrey Dong, Diansheng TI Menu Labeling Fills the Gaps in Consumers' Knowledge of the Calorie Content of Restaurant Foods SO AGRIBUSINESS LA English DT Article ID FULL-SERVICE RESTAURANTS; NUTRITION INFORMATION; CHAIN RESTAURANTS AB Restaurant foods are typically higher in calories than meals consumed at home. A goal of the 2010 Patient Protection and Affordable Care Act is to encourage healthier food choices at restaurants by providing consumers with information about the calorie content of menu items. However, doesn't a well-informed consumer already know that a 12-piece bucket of chicken has more calories than a salad? Following research in marketing science and behavioral economics, we evaluate a representative consumer's ability to discriminate between low-calorie and high-calorie menu items using only some basic rules of thumb nutrition knowledge. Results reveal the extent to which menu labeling may increase consumers' knowledge about the calories in restaurant foods. [EconLit Classifications: I18; D83; L81]. Published 2015. This article is U.S. Government work and is in the public domain in the USA. C1 [Stewart, Hayden; Hyman, Jeffrey; Dong, Diansheng] ERS, USDA, Washington, DC 20250 USA. RP Stewart, H (reprint author), ERS, USDA, 1400 Independence Ave SW,Mail Stop 1800, Washington, DC 20250 USA. EM hstewart@ers.usda.gov; jhyman@ers.usda.gov; ddong@ers.usda.gov NR 32 TC 0 Z9 0 U1 3 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0742-4477 EI 1520-6297 J9 AGRIBUSINESS JI Agribusiness PD FAL PY 2015 VL 31 IS 4 BP 491 EP 506 DI 10.1002/agr.21417 PG 16 WC Agricultural Economics & Policy; Economics; Food Science & Technology SC Agriculture; Business & Economics; Food Science & Technology GA CU1IM UT WOS:000363274300003 ER PT J AU Belle, AJ Lansing, S Mulbry, W Weil, RR AF Belle, Ashley J. Lansing, Stephanie Mulbry, Walter Weil, Ray R. TI Methane and hydrogen sulfide production during co-digestion of forage radish and dairy manure SO BIOMASS & BIOENERGY LA English DT Article DE Anaerobic digestion; Biogas; Cover crop; Biochemical methane potential (BMP); Raphanus sativus L ID ANAEROBIC-DIGESTION; CATTLE MANURE; BIOGAS PRODUCTION; ENERGY CROPS; HARVEST TIME; COVER CROPS; FOOD WASTE; YIELD; BATCH; RESIDUES AB Forage radish, a winter cover crop, was investigated as a co-substrate to increase biogas production from dairy manure-based anaerobic digestion. Batch digesters (300 cm(3)) were operated under mesophilic conditions during two experiments (BMP1; BMP2). In BMP1, the effect of co-digesting radish and manure on CH4 and H2S production was determined by increasing the mass fraction of fresh above-ground radish in the manure-based co-digestion mixture from 0 to 100%. Results showed that forage radish had 1.5-fold higher CH4 potential than dairy manure on a volatile solids basis. While no synergistic effect on CH4 production resulted from co-digestion, increasing the radish fraction in the co-digestion mixture significantly increased CH4 production. Initial H2S production increased as the radish fraction increased, but the sulfur-containing compounds were rapidly utilized, resulting in all treatments having similar H2S concentrations (0.10-0.14%) and higher CH4 content (48-70%) in the biogas over time. The 100% radish digester had the highest specific CH4 yield (372 +/- 12 L kg(-1) VS). The co-digestion mixture containing 40% radish had a lower specific CH4 yield (345 +/- 2 L kg(-1) VS) but also showed significantly less H2S production at start-up and high quality biogas (58% CH4). Results from BMP2 showed that the radish harvest date (October versus December) did not significantly influence radish C:N mass ratios or CH4 production during co-digestion with dairy manure. These results suggest that dairy farmers could utilize forage radish, a readily available substrate that does not compete with food supply, to increase CH4 production of manure digesters in the fall/winter. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Belle, Ashley J.; Lansing, Stephanie; Weil, Ray R.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Mulbry, Walter] ARS, USDA, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. RP Lansing, S (reprint author), Univ Maryland, Dept Environm Sci & Technol, 1449 Anim Sci Ag Engn Bldg, College Pk, MD 20742 USA. EM abelle@umd.edu; slansing@umd.edu; walter.mulbry@ars.usda.gov; rweil@umd.edu FU Northeast Sun Grant Initiative [NE10-040]; USDA personnel FX This research was supported by the Northeast Sun Grant Initiative (NE10-040). Our sincere appreciation is extended to the University of Maryland's (UMD) Waste to Energy/Water Quality Laboratory, especially Veronika Zhiteneva, Holly Bowen, Kayoko Iwata, Akua Nkrumah, Freddy Witarsa, Katherine Klavon, Andrew Moss, and Maria Sol Lisboa. We would also like to thank the USDA personnel and the BARC dairy farm crew for their support and assistance. NR 33 TC 1 Z9 1 U1 5 U2 13 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD SEP PY 2015 VL 80 BP 44 EP 51 DI 10.1016/j.biombioe.2015.04.029 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CU3KF UT WOS:000363423100006 ER PT J AU Kimura, E Fransen, SC Collins, HP Guy, SO Johnston, WJ AF Kimura, Emi Fransen, Steven C. Collins, Harold P. Guy, Stephen O. Johnston, William J. TI Breaking seed dormancy of switchgrass (Panicum virgatum L.): A review SO BIOMASS & BIOENERGY LA English DT Review DE Switchgrass; Panicum virgatum L.; Seed dormancy; Germination; Stand establishment ID SEASON FORAGE GRASSES; BIG BLUESTEM; TILLER NUMBER; BIOMASS YIELD; PLANT-GROWTH; LAKE ERIE; GERMINATION; REGISTRATION; CULTIVARS; EMERGENCE AB Switchgrass (Panicum virgatum L.) is a perennial warm-season grass identified as a model species for bioenergy feedstock production. Established switchgrass stands are very resilient to the environmental fluctuations; however, seed dormancy and weak seedling vigor make establishment difficult. Breaking seed dormancy of switchgrass is a first step to reduce risk of establishment failure and the costs associated with reseeding. Many studies have reported a myriad of methods to break seed dormancy of switchgrass, including chemical, mechanical, thermal, and hormonal seed treatments. Length of seed storage, storage conditions (e.g., temperature and humidity), and prior soil conditions (e.g., soil salinity and fertilizer rates) affect switchgrass seed dormancy. Strong interactions exist among germination, seedling emergence, and soil conditions; therefore, treated seeds tested in soil media will generate more accurate results following dormancy breaking techniques. This paper reviews the current methods used to break switchgrass seed dormancy. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Kimura, Emi] Texas AgriLife Extens Serv, Dept Soil & Crop Sci, Vernon, TX 76384 USA. [Fransen, Steven C.] Irrigated Agr Res & Extens Ctr, Dept Crop & Soil Sci, Prosser, WA 99350 USA. [Collins, Harold P.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Guy, Stephen O.; Johnston, William J.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Kimura, E (reprint author), Texas AgriLife Extens Serv, Dept Soil & Crop Sci, Vernon, TX 76384 USA. EM emi.kimura@ag.tamu.edu; fransen@wsu.edu; hal.collins@ars.usda.gov; sguy@wsu.edu; wjohnston@wsu.edu FU Agricultural Research Service under the GRACEnet Project; USDA Agriculture and Food Research Initiative Carbon Sequestration and Sustainable Bioenergy Production program [2011-67009-300001] FX This publication is based on work supported by the Agricultural Research Service under the GRACEnet Project and grant from the USDA Agriculture and Food Research Initiative Carbon Sequestration and Sustainable Bioenergy Production program (2011-67009-300001). NR 93 TC 0 Z9 1 U1 14 U2 33 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD SEP PY 2015 VL 80 BP 94 EP 101 DI 10.1016/j.biombioe.2015.04.036 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CU3KF UT WOS:000363423100011 ER PT J AU Knoll, JE Johnson, JM Huang, P Lee, RD Anderson, WF AF Knoll, Joseph E. Johnson, Jennifer M. Huang, Ping Lee, R. Dewey Anderson, William F. TI Effects of delayed winter harvest on biomass yield and quality of napiergrass and energycane SO BIOMASS & BIOENERGY LA English DT Article DE Simultaneous saccharification and fermentation; Cellulosic ethanol; Nitrogen; Cenchrus purpureus (Schumach.) Morrone; Saccharum hyb ID REED CANARY GRASS; MISCANTHUS; GENOTYPES; USA; COMBUSTION; SUGARCANE; CROPS; TIME; DATE AB Napiergrass (Cenchrus purpureus (Schumach.) Morrone) and energycane (Saccharum hyb.) are perennial grasses that are well-suited for biomass production in the southeastern USA. The purpose of this study was to determine the effects of delayed winter harvest on biomass yield and quality of these grasses. The study was conducted on two adjacent sites near Midville, GA. Each site used a split-plot design with four replications, with species as the main plot, and harvest times (December, January, or February) as subplots. Dry matter (DM) yields were measured by mechanical harvesting, and a sample of biomass was taken from each harvest for determination of ethanol production by simultaneous saccharification and fermentation (SSF). Biomass moisture, N, P, K, and ash mass fractions were also measured. Energycane DM yields were stable from December (46.8 Mg ha(-1)) to January (42.9 Mg ha(-1)), but then declined (36.8 Mg ha(-1)), while napiergrass yields declined sharply from December (47.0 Mg ha(-1)) to January (35.0 Mg ha(-1)). Napiergrass moisture mass fraction was reduced by an average of 18% in February harvests compared to December. Mass fractions of N, K, and ash tended to decrease with later harvesting, but sometimes increased due to changes in biomass composition. Delaying harvest of napiergrass from December to January reduced N removal by an average of 144 kg ha(-1), while delaying harvest of energycane to February reduced N removal by an average of 54 kg ha(-1). In SSF, later-harvested energycane produced less ethanol per unit of DM while napiergrass was less affected by harvest date. Published by Elsevier Ltd. C1 [Knoll, Joseph E.; Anderson, William F.] ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Johnson, Jennifer M.; Huang, Ping; Lee, R. Dewey] Univ Georgia Tifton Campus, Dept Crop & Soil Sci, Tifton, GA 31793 USA. RP Knoll, JE (reprint author), ARS, USDA, Crop Genet & Breeding Res Unit, POB 748, Tifton, GA 31793 USA. EM Joe.Knoll@ars.usda.gov; jmj0027@auburn.edu; pzh0001@auburn.edu; deweylee@uga.edu; Bill.Anderson@ars.usda.gov FU U.S. Department of Energy [DE-FG36-08G088036] FX The authors would like to thank Robert Pippin and Anthony Black (University of Georgia) for assistance in planting, maintaining, and harvesting the field plots. Freddie Cheek and Tony Howell (USDA-ARS) also assisted with harvesting. Ron Hector and Bruce Dien (USDA-ARS) provided the xylose-fermenting yeast (YRH400). This research was funded by U.S. Department of Energy award DE-FG36-08G088036. NR 24 TC 1 Z9 1 U1 3 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD SEP PY 2015 VL 80 BP 330 EP 337 DI 10.1016/j.biombioe.2015.06.018 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CU3KF UT WOS:000363423100036 ER PT J AU Heikema, AP Islam, Z Horst-Kreft, D Huizinga, R Jacobs, BC Wagenaar, JA Poly, F Guerry, P van Belkum, A Parker, CT Endtz, HP AF Heikema, A. P. Islam, Z. Horst-Kreft, D. Huizinga, R. Jacobs, B. C. Wagenaar, J. A. Poly, F. Guerry, P. van Belkum, A. Parker, C. T. Endtz, H. P. TI Campylobacter jejuni capsular genotypes are related to Guillain-Barre syndrome SO CLINICAL MICROBIOLOGY AND INFECTION LA English DT Article DE Campylobacter jejuni; capsular genotype; Guillain-Barre syndrome; lipo-oligosaccharide; multilocus sequence typing ID SOUTH-AFRICA; RISK-FACTORS; STRAINS; INFECTIONS; GENES; COLI; NETHERLANDS; PREVALENCE; BANGLADESH; VIRULENCE AB In about one in a thousand cases, a Campylobacter jejuni infection results in the severe polyneuropathy Guillain-Barre syndrome (GBS). It is established that sialylated lipo-oligosaccharides (LOS) of C. jejuni are a crucial virulence factor in GBS development. Frequent detection of C. jejuni with sialylated LOS in stools derived from patients with uncomplicated enteritis implies that additional bacterial factors should be involved. To assess whether the polysaccharide capsule is a marker for GBS, the capsular genotypes of two geographically distinct GBS-associated C. jejuni strain collections and an uncomplicated enteritis control collection were determined. Capsular genotyping of C. jejuni strains from the Netherlands revealed that three capsular genotypes, HS1/44c, HS2 and HS4c, were dominant in GBS-associated strains and capsular types HS1/44c and HS4c were significantly associated with GBS (p 0.05 and p 0.01, respectively) when compared with uncomplicated enteritis. In a GBS-associated strain collection from Bangladesh, capsular types HS23/36c, HS19 and HS41 were most prevalent and the capsular types HS19 and HS41 were associated with GBS (p 0.008 and p 0.02, respectively). Next, specific combinations of the LOS class and capsular genotypes were identified that were related to the occurrence of GBS. Multilocus sequence typing revealed restricted genetic diversity for strain populations with the capsular types HS2, HS19 and HS41. We conclude that capsular types HS1/44c, HS2, HS4c, HS19, HS23/36c and HS41 are markers for GBS. Besides a crucial role for sialylated LOS of C. jejuni in GBS pathogenesis, the identified capsules may contribute to GBS susceptibility. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved. C1 [Heikema, A. P.; Horst-Kreft, D.; Endtz, H. P.] Univ Med Ctr Rotterdam, Erasmus MC, Dept Med Microbiol & Infect Dis, NL-3015 CE Rotterdam, Netherlands. [Islam, Z.; Endtz, H. P.] Int Ctr Diarrhoeal Dis Res, Dhaka, Bangladesh. [Huizinga, R.; Jacobs, B. C.] Univ Med Ctr Rotterdam, Erasmus MC, Dept Immunol, NL-3015 CE Rotterdam, Netherlands. [Jacobs, B. C.] Univ Med Ctr Rotterdam, Erasmus MC, Dept Neurol, NL-3015 CE Rotterdam, Netherlands. [Wagenaar, J. A.] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Lelystad, Netherlands. [Wagenaar, J. A.] Wageningen UR, Cent Vet Inst, Lelystad, Netherlands. [Poly, F.; Guerry, P.] Naval Med Res Ctr, Silver Spring, MD USA. [van Belkum, A.] BioMerieux, R&D Microbiol, La Balme Les Grottes, France. [Parker, C. T.] ARS, Prod Safety & Microbiol Res Unit, USDA, Albany, CA USA. [Endtz, H. P.] Fdn Merieux, Lyon, France. RP Heikema, AP (reprint author), Univ Med Ctr Rotterdam, Erasmus MC, Dept Med Microbiol & Infect Dis, S Gravendijkwal 230, NL-3015 CE Rotterdam, Netherlands. EM a.heikema@erasmusmc.nl FU Navy Work Unit [6000.RAD1.DA3.A0308]; United States Department of Agriculture, Agricultural Research Service CRIS [5325-42000-047] FX This project was supported in part by Navy Work Unit 6000.RAD1.DA3.A0308 and the United States Department of Agriculture, Agricultural Research Service CRIS projects 5325-42000-047. NR 37 TC 2 Z9 2 U1 3 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1198-743X EI 1469-0691 J9 CLIN MICROBIOL INFEC JI Clin. Microbiol. Infect. PD SEP PY 2015 VL 21 IS 9 AR UNSP 852.e1 DI 10.1016/j.cmi.2015.05.031 PG 9 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA CU4HL UT WOS:000363488400010 PM 26070960 ER PT J AU Chandrasekaran, SR Kunwar, B Moser, BR Rajagopalan, N Sharma, BK AF Chandrasekaran, Sriraam R. Kunwar, Bidhya Moser, Bryan R. Rajagopalan, Nandakishore Sharma, Brajendra K. TI Catalytic Thermal Cracking of Postconsumer Waste Plastics to Fuels. 1. Kinetics and Optimization SO ENERGY & FUELS LA English DT Article ID FLUIDIZED-BED REACTOR; RESPONSE-SURFACE METHODOLOGY; HIGH-DENSITY POLYETHYLENE; LIFE-CYCLE ASSESSMENT; DIESEL FUEL; SOLID-WASTE; PYROLYSIS; DEGRADATION; CONVERSION; POLYSTYRENE AB Thermogravimetric analysis (TGA) was used to investigate thermal and catalytic pyrolysis of waste plastics such as prescription bottles (polypropylene/PP), high density polyethylene, landfill liners (polyethylene/PE), packing materials (polystyrene/PS), and foams (polyurethane/PU) into crude plastic oils. In the first phase of this investigation, a statistical design experiments approach identified reaction temperature and time as the most important factors influencing product oil yield. Kinetic parameters including activation energy determined for both catalytic and noncatalytic processes showed a reduction in activation energy for the catalytic reactions. In the second phase, the interactions of reaction temperature and time with a number of catalysts were investigated to determine the effect on the yield of crude plastic oil. It was found that Y-zeolites increased conversion at reduced temperature for PP and PE while spent fluid catalytic cracking and sulfated zirconia catalysts supported pyrolytic decomposition of PS and PU foams. Response surface methodology (RSM) was utilized to optimize TGA conditions for pyrolytic decomposition of PP. The results were then validated through batch scale experiments, and the resulting crude oils were characterized and distilled into motor gasoline, diesel #1, diesel #2, and vacuum gas oil fractions. Catalysts enhanced cracking at lower temperatures and narrowed the molecular weight (hydrocarbon) distribution in the crude oils. Chemical characterization of the crude oils indicated an increased gasoline-range fraction in oils obtained in the presence of catalyst while the distillate fractions were more evenly distributed among gasoline-range and diesel-range hydrocarbons in the absence of catalyst. The distillates obtained were characterized for fuel properties, elemental composition, boiling point, and molecular weight distribution. The fuel properties of the diesel-range distillate (diesel fraction) were comparable to those of ultralow sulfur diesel (ULSD). C1 [Chandrasekaran, Sriraam R.; Kunwar, Bidhya; Rajagopalan, Nandakishore; Sharma, Brajendra K.] Univ Illinois, Illinois Sustainable Technol Ctr, Prairie Res Inst, Urbana, IL 61820 USA. [Moser, Bryan R.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Chandrasekaran, SR (reprint author), Univ Illinois, Illinois Sustainable Technol Ctr, Prairie Res Inst, Urbana, IL 61820 USA. EM schandr@illinois.edu; bksharma@illinois.edu FU Environmental Research and Education Foundation (EREF); Illinois Hazardous Waste Research Fund FX The authors thank the financial support from Environmental Research and Education Foundation (EREF) and part funding from the Illinois Hazardous Waste Research Fund. The authors also thank the Noyce Laboratory and Materials Research Laboratory facilities for access to the instruments for chemical characterization. In addition, the authors thank Dheeptha Murali for her assistance in conducting the TGA experiments. NR 39 TC 5 Z9 5 U1 2 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD SEP PY 2015 VL 29 IS 9 BP 6068 EP 6077 DI 10.1021/acs.energyfuels.5b01083 PG 10 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA CT8LN UT WOS:000363068200057 ER PT J AU Roesch, FA Coulston, JW Van Deusen, PC Podlaski, R AF Roesch, Francis A. Coulston, John W. Van Deusen, Paul C. Podlaski, Rafal TI Evaluation of Image-Assisted Forest Monitoring: A Simulation SO FORESTS LA English DT Article DE forest monitoring; sample design; estimation; auxiliary information; remote sensing ID LAND-USE; CONVERSION RATES; INVENTORY DATA; COMPONENTS; DESIGN; COVER; TIME AB Fiscal uncertainties can sometimes affect national continuous forest monitoring efforts. One solution of interest is to lengthen the time it takes to collect a full set of plot data from five to 10 years in order to reduce costs. Here, we investigate using ancillary information to partially offset this proposed solution's negative effects. We focus our discussion on the corresponding number of years between measurements of each plot while we investigate how thoroughly the detrimental effects of the reduced sampling effort can be ameliorated with change estimates obtained from temporally-dense remotely-sensed images. We simulate measured plot data under four sampling error structures, and we simulate remotely-sensed change estimates under three reliability assumptions, integrated with assumptions about the additional unobserved growth resulting from the lengthened observation window. We investigate a number of estimation systems with respect to their ability to provide compatible annual estimates of the components of change during years spanned by at least half of the full set of plot observations. We show that auxiliary data with shorter observation intervals can contribute to a significant improvement in estimation. C1 [Roesch, Francis A.] US Forest Serv, Southern Res Stn, USDA, Asheville, NC 28804 USA. [Coulston, John W.] US Forest Serv, Southern Res Stn, USDA, Blacksburg, VA 24060 USA. [Van Deusen, Paul C.] Natl Council Air & Stream Improvement, Mount Washington, MA 01258 USA. [Podlaski, Rafal] Jan Kochanowski Univ Humanities & Sci, Inst Biol, Dept Nat Protect, PL-25406 Kielce, Poland. RP Roesch, FA (reprint author), US Forest Serv, Southern Res Stn, USDA, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM froesch@fs.fed.us; jcoulston@fs.fed.us; pvandeus@gmail.com; rafal.podlaski@ujk.edu.pl NR 21 TC 2 Z9 2 U1 0 U2 6 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD SEP PY 2015 VL 6 IS 9 BP 2897 EP 2917 DI 10.3390/f6092897 PG 21 WC Forestry SC Forestry GA CT1KZ UT WOS:000362558500001 ER PT J AU Kiszonas, AM Fuerst, EP Morris, CF AF Kiszonas, Alecia M. Fuerst, E. Patrick Morris, Craig F. TI Use of Student's t statistic as a phenotype of relative consumption preference of wheat (Triticum aestivum L.) grain SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Wheat; Flavor; Statistics; Quality ID MUS-MUSCULUS L.; GENOME-WIDE ASSOCIATION; VARIETIES; DESIGN AB Whole-grain wheat (Triticum aestivum L) products provide essential nutrients to humans, but bran attributes may hinder consumption. Differences in grain attributes including flavor/aroma can be identified using the house mouse (Mus musculus L.) as a model system. A potential application of this model system is to identify genes or quantitative trait loci (QTLs) conferring consumption-related traits using genetic mapping. These analyses require a quantitative phenotype to test marker trait associations. Two-choice feeding trials are common in consumption studies, but do not provide an independent, quantitative phenotype. The objective of this study was to examine the use of 'check' varieties against which to compare sample varieties to generate Student's t values for use as phenotypic values. Two checks, which had previously been identified as "Yummy" and "yucky" (Y, y) were compared against each sample variety. The resulting t values provided an independent quantitative phenotype of the sample varieties. Published by Elsevier Ltd. C1 [Kiszonas, Alecia M.; Morris, Craig F.] Washington State Univ, USDA ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA. [Fuerst, E. Patrick] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Fuerst, E. Patrick] USDA ARS, Western Wheat Qual Lab, Washington, DC 20250 USA. RP Morris, CF (reprint author), Washington State Univ, USDA ARS, Western Wheat Qual Lab, E-202 Food Qual Bldg, Pullman, WA 99164 USA. EM morrisc@wsu.edu FU Ardent Mills LLC [58-2090-5-010] FX The authors wish to thank Dr. Stephen Guy and his staff for samples. Stacey Sykes and Shawna Vogl assisted in the preparation of the manuscript. Thanks go to Blair Ehlert of the WSU vivarium. Partial financial support was provided by Ardent Mills LLC (Agreement No. 58-2090-5-010). NR 12 TC 2 Z9 2 U1 2 U2 5 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD SEP PY 2015 VL 65 BP 285 EP 289 DI 10.1016/j.jcs.2015.08.003 PG 5 WC Food Science & Technology SC Food Science & Technology GA CU2LS UT WOS:000363355600040 ER PT J AU Wasik, JKC Engstrom, DR Mitchell, CPJ Swain, EB Monson, BA Balogh, SJ Jeremiason, JD Branfireun, BA Kolka, RK Almendinger, JE AF Wasik, J. K. Coleman Engstrom, D. R. Mitchell, C. P. J. Swain, E. B. Monson, B. A. Balogh, S. J. Jeremiason, J. D. Branfireun, B. A. Kolka, R. K. Almendinger, J. E. TI The effects of hydrologic fluctuation and sulfate regeneration on mercury cycling in an experimental peatland SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE methylmercury; peatlands; drought; sulfate deposition; sulfur cycling ID ATOMIC FLUORESCENCE DETECTION; AQUEOUS-PHASE ETHYLATION; SOUTH-CENTRAL ONTARIO; PORE-WATER CHEMISTRY; NEW-JERSEY PINELANDS; METHYLMERCURY PRODUCTION; SOUTHEASTERN CANADA; SEASONAL INFLUENCES; GAS-CHROMATOGRAPHY; MANAGED WETLANDS AB A series of severe droughts during the course of a long-term, atmospheric sulfate-deposition experiment in a boreal peatland in northern Minnesota created a unique opportunity to study how methylmercury (MeHg) production responds to drying and rewetting events in peatlands under variable levels of sulfate loading. Peat oxidation during extended dry periods mobilized sulfate, MeHg, and total mercury (Hg-T) to peatland pore waters during rewetting events. Pore water sulfate concentrations were inversely related to antecedent moisture conditions and proportional to past and current levels of atmospheric sulfate deposition. Severe drying events caused oxidative release of MeHg to pore waters and resulted in increased net MeHg production likely because available sulfate stimulated the activity of sulfate-reducing bacteria, an important group of Hg-methylating bacteria in peatlands. Rewetting events led to increased MeHg concentrations across the peatland, but concentrations were highest in peat receiving elevated atmospheric sulfate deposition. Dissolved Hg-T concentrations also increased in peatland pore waters following drought but were not affected by sulfate loading and did not appear to be directly controlled by dissolved organic carbon mobilization to peatland pore waters. Peatlands are often considered to be sinks for sulfate and Hg-T in the landscape and sources of MeHg. Hydrologic fluctuations not only serve to release previously sequestered sulfate and Hg-T from peatlands but may also increase the strength of peatlands as sources of MeHg to downstream aquatic systems, particularly in regions that have experienced elevated levels of atmospheric sulfate deposition. C1 [Wasik, J. K. Coleman] Univ Wisconsin, Dept Plant & Earth Sci, River Falls, WI 54022 USA. [Engstrom, D. R.; Almendinger, J. E.] Sci Museum Minnesota, St Croix Watershed Res Stn, Marine St Croix, MN USA. [Mitchell, C. P. J.] Univ Toronto Scarborough, Dept Phys & Environm Sci, Toronto, ON, Canada. [Swain, E. B.; Monson, B. A.] Minnesota Pollut Control Agcy, Environm Anal & Outcomes Div, St Paul, MN USA. [Balogh, S. J.] Metropolitan Council Environm Serv, St Paul, MN USA. [Jeremiason, J. D.] Gustavus Adolphus Coll, Dept Chem, St Peter, MN 56082 USA. [Branfireun, B. A.] Univ Western Ontario, Dept Biol, London, ON, Canada. [Kolka, R. K.] US Forest Serv, No Res Stn, USDA, Grand Rapids, MN USA. RP Wasik, JKC (reprint author), Univ Wisconsin, Dept Plant & Earth Sci, River Falls, WI 54022 USA. EM jill.colemanwasik@uwrf.edu RI Mitchell, Carl/A-7212-2008 OI Mitchell, Carl/0000-0001-8538-5138 FU U.S. EPA-Science [R827630]; Great Lakes Commission; Great Lakes Air Deposition program; Minnesota Pollution Control Agency FX Funding for this long-term project came from the U.S. EPA-Science to Achieve Results Program grant R827630, the Great Lakes Commission, Great Lakes Air Deposition program, and the Minnesota Pollution Control Agency. The USDA Forest Service's Northern Research Station provided access to the study site as well as substantial in-kind support. Many individuals assisted with sulfate additions and sample collection including P. Hoff and D. Helwig (Minnesota Pollution Control Agency); A. Stephens, A. Baczynski, and W. Daniels (St. Croix Watershed Research Station); and J. Heissel, C. Green, and J. Westlake (Northern Research Station). We also gratefully acknowledge the support of the analysts and technicians including Y. Nollet (Metropolitan Council Environmental Services), D. Nelson and J. Larson (Northern Research Station), and C. Eckley and M. Collins (University of Toronto, Mississauga). Special thanks go to R. Kyllander and C. Dorrance at the Northern Research Station for the administrative and field assistance throughout the course of the project. The data presented in this paper may be obtained by sending a written request to the corresponding author. NR 74 TC 6 Z9 6 U1 11 U2 50 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD SEP PY 2015 VL 120 IS 9 BP 1697 EP 1715 DI 10.1002/2015JG002993 PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CU2DL UT WOS:000363332900001 ER PT J AU Lawrence, DP Travadon, R Baumgartner, K AF Lawrence, Daniel P. Travadon, Renaud Baumgartner, Kendra TI Diversity of Diaporthe species associated with wood cankers of fruit and nut crops in northern California SO MYCOLOGIA LA English DT Article DE Diaporthaceae; Phomopsis; phylogenetic species recognition; plant pathogens; Vitis ID PHOMOPSIS-VITICOLA; MULTILOCUS STRUCTURE; MATING-TYPES; GRAPEVINES; PHYLOGENY; EVOLUTION; FUNGI; POPULATIONS; COMPLEX; GENUS AB Diaporthe ampelina, causal agent of Phomopsis cane and leaf spot of grapevine (Vitis vinifera L.) is isolated frequently from grapevine wood cankers, causing Phomopsis dieback. The latter disease is associated with four other Diaporthe species, three of which also are reported from hosts other than grape. To better understand the role of this Diaporthe community in Phomopsis dieback of grapevine and the potential for infection routes among alternate hosts, 76 Diaporthe isolates were recovered from wood cankers of cultivated grape, pear, apricot, almond and the wild host willow in four California counties. Isolates were characterized morphologically and assigned to species based on multigene sequence analyses. This study identified eight Diaporthe species from grapevine and one novel taxon from willow, D. benedicti. We report the first findings of D. australafricana and D. novem in North America. Our findings also expand the host ranges of D. ambigua to apricot and willow, D. australafricana to almond and willow, D. chamaeropis to grapevine and willow, D. foeniculina to willow and D. novem to almond. The generalists D. ambigua and D. eres were the most genetically diverse species, based on high nucleotide and haplotypic diversity, followed by the grapevine specialist D. ampelina. Analyses based on multilocus linkage disequilibrium could not reject the hypothesis of random mating for D. ambigua, which is further supported by relatively high haplotypic diversity, reports of both mating types and reports of successful matings in vitro. Pathogenicity assays revealed that D. ampelina was the most pathogenic species to grapevine wood. C1 [Lawrence, Daniel P.; Travadon, Renaud] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Baumgartner, Kendra] ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Baumgartner, K (reprint author), ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. EM Kendra.Baumgartner@ars.usda.gov FU United States Department of Agriculture [2012-51181-19954]; National Institute of Food and Agriculture; Specialty Crop Research Initiative FX Authors thank E. Galarneau and P. Fujiyoshi (USDA-ARS) for technical assistance. Research was financially supported by grant number 2012-51181-19954 to K. Baumgartner from the United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 52 TC 3 Z9 3 U1 4 U2 18 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD SEP-OCT PY 2015 VL 107 IS 5 BP 926 EP 940 DI 10.3852/14-353 PG 15 WC Mycology SC Mycology GA CT6PA UT WOS:000362933700004 PM 26240309 ER PT J AU Czaplewski, RL AF Czaplewski, Raymond L. TI Novel Kalman Filter Algorithm for Statistical Monitoring of Extensive Landscapes with Synoptic Sensor Data SO SENSORS LA English DT Article DE Landsat; MODIS; change detection; square root filter; big data; forest inventory and analysis program; FIA ID FOREST INVENTORY; STRATIFICATION; PLOTS; MODEL AB Wall-to-wall remotely sensed data are increasingly available to monitor landscape dynamics over large geographic areas. However, statistical monitoring programs that use post-stratification cannot fully utilize those sensor data. The Kalman filter (KF) is an alternative statistical estimator. I develop a new KF algorithm that is numerically robust with large numbers of study variables and auxiliary sensor variables. A National Forest Inventory (NFI) illustrates application within an official statistics program. Practical recommendations regarding remote sensing and statistical issues are offered. This algorithm has the potential to increase the value of synoptic sensor data for statistical monitoring of large geographic areas. C1 US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80521 USA. RP Czaplewski, RL (reprint author), US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80521 USA. EM raymond_czaplewski@environmetrika.org FU Interior West Forest Inventory and Analysis (FIA) Program, Rocky Mountain Research Station, U.S. Forest Service, Ogden, UT FX This study was funded by the Interior West Forest Inventory and Analysis (FIA) Program, Rocky Mountain Research Station, U.S. Forest Service, Ogden, UT. G.G. Moisen provided unwavering support for this study. T.S. Frescino and J.A. Blackard contributed database and GIS services. Hundreds of dedicated biological technicians throughout the years endured considerable hardships to collect FIA field data, which is the foundation of the case study. The case study would not have been possible without sustained support from the Remote Sensing Application Center, U.S. Forest Service, Salt Lake City, UT. E. Volkman, who is the Deputy Program Manager for the Interior West FIA Program, conducted the policy review and contributed numerous suggestions that improved the manuscript. L.S. Baggot, who is the Station Biometrician for the Rocky Mountain Research Station, provided the internal biometrics review. Three anonymous reviewers recruited by an editor for Sensors greatly improved the substance and communication of this research. The relationship of the KF estimator to the univariate g-weighted regression estimator was suggested by one of these reviewers. NR 18 TC 0 Z9 0 U1 1 U2 2 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD SEP PY 2015 VL 15 IS 9 BP 23589 EP 23617 DI 10.3390/s150923589 PG 29 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA CT0UH UT WOS:000362512200132 PM 26393588 ER PT J AU Eggleston, G Lima, I AF Eggleston, Gillian Lima, Isabel TI Sustainability Issues and Opportunities in the Sugar and Sugar-Bioproduct Industries SO SUSTAINABILITY LA English DT Review DE renewable sugar crops; biomass; advanced biofuels; bioproducts; bagasse; extraneous matter ID GRANULAR ACTIVATED CARBONS; AGRICULTURAL BY-PRODUCTS; PROXIMATE ANALYSIS; POTENTIAL BIOMASS; CANE BAGASSE; RAW SUGAR; PART I; ETHANOL; ENERGY; BIOENERGY AB Like many other industries, the sugar and sugar-bioproduct industries are facing important sustainability issues and opportunities. The relatively low and fluctuating profit for sugar, surpluses of sugar, world-wide trend to produce alternative, renewable bio-based fuels and chemicals to those derived from petroleum and reduce greenhouse gases, water- and energy-intensive factories and refineries, and increased consumer demands for sustainably manufactured products are putting pressure on the industries to diversify for sustainability. Sugar crops, including sugar and energy cane (Saccharum officinarum), sugar and energy beets (Beta vulgaris), and sweet sorghum (Sorghum bicolor L. Moench), are excellent, renewable biomass feedstocks because of their availability, their being amongst the plants that give the highest yields of carbohydrates per hectare, and high sugar contents. While much research has been focused on conversion technologies for advanced biofuels and bioproducts, attention is now focused on developing sustainable supply chains of sugar feedstocks for the new, flexible biorefineries, with customers wanting maximum feedstock reliability and quality, while minimizing cost. All biomass from sugar crops are potential feedstocks. The cogeneration of bioelectricity from bagasse and leaf residues is being increasingly manufactured in more countries and, due to the high carbon content of bagasse and leaves, can also be converted into value-added products such as biochar. Sugar crops are superior feedstocks for the production of platform chemicals for the manufacture of a range of end-products, e.g., bioplastics, chemicals, and biomaterials. In several countries and regions, green sustainability criteria are now in place and have to be met to count against national biofuel targets. Processes to convert high-fiber sugar crop biomass into biofuel have been developed but there has only been limited commercialization at the large-scale. C1 [Eggleston, Gillian; Lima, Isabel] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov; isabel.lima@ars.usda.gov NR 112 TC 3 Z9 3 U1 14 U2 47 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD SEP PY 2015 VL 7 IS 9 BP 12209 EP 12235 DI 10.3390/su70912209 PG 27 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA CT1JA UT WOS:000362553400044 ER PT J AU Karlen, DL Rice, CW AF Karlen, Douglas L. Rice, Charles W. TI Soil Degradation: Will Humankind Ever Learn? SO SUSTAINABILITY LA English DT Editorial Material DE soil health; soil quality; sustainable intensification; soil biology; erosion; soil organic matter; carbon sequestration ID QUALITY; SUSTAINABILITY; CHALLENGES; HISTORY; LAND AB Soil degradation is a global problem caused by many factors including excessive tillage, inappropriate crop rotations, excessive grazing or crop residue removal, deforestation, mining, construction and urban sprawl. To meet the needs of an expanding global population, it is essential for humankind to recognize and understand that improving soil health by adopting sustainable agricultural and land management practices is the best solution for mitigating and reversing current soil degradation trends. This research editorial is intended to provide an overview for this Special Issue of Sustainability that examines the global problem of soil degradation through reviews and recent research studies addressing soil health in Africa, Australia, China, Europe, India, North and South America, and Russia. Two common factorssoil erosion and depletion of soil organic matter (SOM)emerge as consistent indicators of how the thin layer covering the planet that stands between us and starvation is being degraded. Soil degradation is not a new problem but failing to acknowledge, mitigate, and remediate the multiple factors leading to it is no longer a viable option for humankind. We optimistically conclude that the most promising strategies to mitigate soil degradation are to select appropriate land uses and improve soil management practices so that SOM is increased, soil biology is enhanced, and all forms of erosion are reduced. Collectively, these actions will enable humankind to take care of the soil so it can take care of us. C1 [Karlen, Douglas L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Rice, Charles W.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. RP Karlen, DL (reprint author), USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM Doug.Karlen@ars.usda.gov; cwrice@ksu.edu NR 33 TC 4 Z9 4 U1 24 U2 71 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD SEP PY 2015 VL 7 IS 9 BP 12490 EP 12501 DI 10.3390/su70912490 PG 12 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA CT1JA UT WOS:000362553400058 ER PT J AU Zhang, SX Ma, YY Guo, HW Wan, WT Xue, K AF Zhang, Shixiu Ma, Yiyi Guo, Hongwei Wan, Wentao Xue, Kun TI Diets high in carbohydrate may not be appropriate for rs328 G carriers with the metabolic syndrome SO ASIA PACIFIC JOURNAL OF CLINICAL NUTRITION LA English DT Article DE gene-by-diet interaction; carbohydrate; lipoprotein lipase; metabolic syndrome; insulin resistance ID TISSUE LIPOPROTEIN-LIPASE; STEROL REGULATORY ELEMENT; BODY-MASS INDEX; INSULIN-RESISTANCE; GENE-EXPRESSION; WEIGHT-LOSS; FAT; MICE; APOLIPOPROTEIN; PREVALENCE AB The objective of this study was to test how the genetic polymorphisms located within the lipoprotein lipase (LPL) locus would modulate the relationship between a diet high in carbohydrate and insulin resistance related traits in metabolic syndrome adults. A one year nutritional intervention study focusing on education to increase dietary intake of whole grain, vegetable and fruit, and to reduce the intake of sodium, simple sugar and dietary fat (especially cooking oil and pork lard) was conducted. Two districts in Shanghai, China were randomly selected to be the intervention and control group, and patients (n=235) with metabolic syndrome within these two districts were selected based on a multistage sampling method. Fasting glucose was reduced in rs328 CC homozygotes (p=0.028) but not G carriers (p=0.686) within the intervention group. Also an ancillary study with greater statistical power by combining the baseline measurements across both the intervention and control groups was conducted to test the cross-sectional statistical interactions between carbohydrate/fat and lipoprotein lipase genotypes for homeostasis model assessment of insulin resistance/insulin/fasting glucose. Increased carbohydrate intakes were positively associated with homeostasis model assessment of insulin resistance and insulin in rs328 G carriers but not CC homozygotes (p for interaction was 0.025). These results indicate that diet high in carbohydrate may not be suitable for metabolic syndrome rs328 G carriers, calling for the development of personalized dietary intervention for metabolic syndrome subjects. C1 [Zhang, Shixiu; Ma, Yiyi; Guo, Hongwei; Wan, Wentao; Xue, Kun] Fudan Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Shanghai 200032, Peoples R China. [Zhang, Shixiu] Shandong Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Jinan 250100, Peoples R China. [Ma, Yiyi] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. RP Guo, HW (reprint author), Fudan Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, 130 Dongan Rd, Shanghai 200032, Peoples R China. EM hwguo@shmu.edu.cn FU Danone Dietary Nutrition for Research and Teaching FX The study was funded by Danone Dietary Nutrition for Research and Teaching. The authors' contributions were as follows: Zhang Shixiu conducted the research, and made substantial contributions to analysis and interpretation of data and drafting of the article. Ma Yiyi participated in the research, reviewed, edited the manuscript and gave critical revision for important intellectual content. Guo Hongwei designed, supervised research, reviewed and edited the manuscript. Wan Wentao contributed to biochemical and SNP tests. Xue Kun contributed to dietary records collection and anthropometric measurement. All authors finally approved the manuscript version to be published. We also thank to the study participants for their cooperation throughout the study. NR 38 TC 1 Z9 1 U1 0 U2 2 PU H E C PRESS, HEALTHY EATING CLUB PTY LTD PI MCKINNON PA PO BOX 4121, MCKINNON, VIC 3204, AUSTRALIA SN 0964-7058 J9 ASIA PAC J CLIN NUTR JI Asia Pac. J. Clin. Nutr. PD SEP PY 2015 VL 24 IS 3 BP 546 EP 554 DI 10.6133/apjcn.2015.24.3.17 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CT6JP UT WOS:000362919600025 PM 26420199 ER PT J AU Fujii, S Srivastava, V Hegde, A Kondo, Y Shen, LL Hoshino, K Gonzalez, Y Wang, J Sasai, K Ma, XT Katayama, H Estecio, MR Hamilton, SR Wistuba, I Issa, JPJ Sen, S AF Fujii, Satoshi Srivastava, Vibhuti Hegde, Apurva Kondo, Yutaka Shen, Lanlan Hoshino, Koyu Gonzalez, Yvette Wang, Jin Sasai, Kaori Ma, Xiaotu Katayama, Hiroshi Estecio, Marcos R. Hamilton, Stanley R. Wistuba, Ignacio Issa, Jean-Pierre J. Sen, Subrata TI Regulation of AURKC expression by CpG island methylation in human cancer cells SO TUMOR BIOLOGY LA English DT Article DE Aurora kinase-C; CpG methylation; Epigenetic; PLZF; Colon adenocarcinoma; Cell lines ID DEACETYLASE INHIBITOR TRICHOSTATIN; CHROMOSOMAL PASSENGER PROTEIN; AURORA B EXPRESSION; ANTICANCER DRUGS; DNA METHYLATION; GENE-EXPRESSION; A KINASE; C KINASE; OVEREXPRESSION; TRANSCRIPTION AB AURKC, a member of the Aurora kinase gene family, is highly expressed in testis but is either moderately expressed or repressed in most somatic cells. Varying expression of AURKC has been observed in human cancers, but the underlying mechanisms of differential expression have been investigated only to a limited extent. We investigated the role of promoter CpG methylation in the regulation of AURKC gene expression in human cancer cells, in relation to a recently reported AURKC transcription repressor PLZF/ZBTB16, implicated in transformation and tumorigenesis. AURKC and PLZF/ZBTB16 expression profiles were investigated in reference to CpG methylation status on the AURKC promoter experimentally, and also in The Cancer Genome Atlas (TCGA) dataset involving multiple cancer types. AURKC promoter showed dense to moderate hypermethylation correlating with low to moderate expression of the gene in normal somatic cells and cancer cell lines, while testis with high expression revealed marked hypo-methylation. Treatment with the demethylating agent, 5-aza-dC, but not the histone deacetylase (HDAC) inhibitor, TSA, led to elevated expression in cancer cell lines, indicating that promoter DNA methylation negatively regulates AURKC expression. High expression of PLZF in PLZF-transfected cells treated with 5-aza-dC only partially repressed expression of AURKC despite 5-aza-dC also inducing elevated PLZF expression. Analyses of the TCGA data showed differential expression of AURKC in multiple cancer types and stronger correlation of AURKC expression with CpG methylation compared to PLZF levels. These findings demonstrate that differential promoter CpG methylation is an important mechanism regulating AURKC expression in cancer cells. C1 [Fujii, Satoshi] Natl Canc Ctr Kashiwa, Res Ctr Innovat Oncol, Div Pathol, Kashiwa, Chiba 2778577, Japan. [Srivastava, Vibhuti; Hegde, Apurva; Gonzalez, Yvette; Wang, Jin; Wistuba, Ignacio; Sen, Subrata] Univ Texas MD Anderson Canc Ctr, Dept Translat Mol Pathol, Houston, TX 77030 USA. [Srivastava, Vibhuti] Univ Texas MD Anderson Canc Ctr, Sch Hlth Profess, Mol Genet Technol Program, Houston, TX 77030 USA. [Hegde, Apurva] Univ Texas Hlth Sci Ctr Houston, Grad Sch Biomed Sci, Program Human & Mol Genet, Houston, TX 77030 USA. [Kondo, Yutaka] Aichi Canc Ctr, Res Inst, Div Epigen, Chikusa Ku, Nagoya, Aichi 4648681, Japan. [Shen, Lanlan] Baylor Coll Med, USDA, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Hoshino, Koyu] Kumamoto Univ, Grad Sch Med Sci, Dept Hematol & Clin Rheumatol, Kumamoto 8608556, Japan. [Sasai, Kaori; Katayama, Hiroshi] Okayama Univ, Sch Med, Dept Mol Genet, Okayama 7008558, Japan. [Ma, Xiaotu] Univ Texas Dallas, Dept Mol & Cell Biol, Ctr Syst Biol, Richardson, TX 75080 USA. [Estecio, Marcos R.] Univ Texas MD Anderson Canc Ctr, Dept Epigenet & Mol Carcinogenesis, Houston, TX 77030 USA. [Hamilton, Stanley R.] Univ Texas MD Anderson Canc Ctr, Div Pathol & Lab Med, Houston, TX 77030 USA. [Issa, Jean-Pierre J.] Temple Univ, Fels Inst Canc Res & Mol Biol, Philadelphia, PA 19140 USA. RP Sen, S (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Translat Mol Pathol, Unit 2951,Life Sci Plaza Bldg, Houston, TX 77030 USA. EM ssen@mdanderson.org FU National Institutes of Health [RO1CA89716, UO1CA85078]; University Cancer Foundation of MD Anderson Cancer Center FX This work was supported by grants from the National Institutes of Health, Grant RO1CA89716 and UO1CA85078, and the University Cancer Foundation of MD Anderson Cancer Center (to SS). We thank Dr. Ronald M. Evans for providing the pCMX-HA-PLZF construct. NR 42 TC 1 Z9 1 U1 1 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1010-4283 EI 1423-0380 J9 TUMOR BIOL JI Tumor Biol. PD SEP PY 2015 VL 36 IS 10 BP 8147 EP 8158 DI 10.1007/s13277-015-3553-5 PG 12 WC Oncology SC Oncology GA CT7CD UT WOS:000362969900090 PM 25990457 ER PT J AU Schmidt, WF Kim, MS Nguyen, JK Qin, J Chao, K Broadhurst, CL Shelton, DR AF Schmidt, W. F. Kim, M. S. Nguyen, J. K. Qin, J. Chao, K. Broadhurst, C. L. Shelton, D. R. TI Continous gradient temperature Raman Spectroscopy identifies flexible sites in proline and alanine peptides SO VIBRATIONAL SPECTROSCOPY LA English DT Article DE Gradient temperature Raman spectroscopy; Proline; Alanine; Peptide structure; Raman spectroscopy; Differential scanning calorimetry ID THERMOPHILIC PROTEINS; SECONDARY STRUCTURE; OPTICAL-ACTIVITY; AMINO-ACIDS; SPECTRA; THERMOSTABILITY; CONFORMATIONS; STABILITY; DYNAMICS AB Continuous gradient temperature Raman spectroscopy (GTRS) applies the temperature gradients utilized in differential scanning calorimetry (DSC) to Raman spectroscopy, providing a straightforward technique to identify molecular rearrangements that occur near phase transitions. Herein we apply GTRS and DSC to the solid dipeptides Ala-Pro, Pro-Ala, and the mixture Ala-Pro/Pro-Ala 2:1. A simple change in residue order resulted in dramatic changes in thermal stability and properties. Characteristic Pro vibrations were observed at similar to 75 degrees C higher temperature in Pro-Ala than Ala-Pro. The appearance/disappearance of characteristic vibrational modes with increasing temperature showed that a double peak in the Ala-Pro major phase transition (174-184 degrees C) was due to a gauche to anti 165 rotation of H3C-C*-NH3 about C*. CH2 rocking and wagging frequencies present in Pro-Ala were not observed in Ala-Pro. For Ala-Pro, the Ala +NH3, and Pro COO- sites were flexible whereas the Pro ring moiety was not; since the O=C N (-C)(2) amide bond is planar the C-N-C moiety keeps the Pro ring rigid. For Pro-Ala, CH2 sites in the Pro ring were flexible and the O=C-NH amide bond is perpendicular to the Pro ring. Since the mass of the Pro ring is significantly larger than the mass of the flexible Ala +NH3 moiety, Pro-Ala absorbs more thermal energy, corresponding to a higher phase transition temperature (240-260 degrees C). Ala-Pro, Pro-Ala, and Ala-Pro/Pro-Ala 2:1 exhibited alpha-helix, beta-sheet, alpha-helix secondary structure conformations, respectively. Published by Elsevier B.V. C1 [Schmidt, W. F.; Kim, M. S.; Nguyen, J. K.; Qin, J.; Chao, K.; Broadhurst, C. L.; Shelton, D. R.] ARS, Environm Microbiol & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Qin, J.; Broadhurst, C. L.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Schmidt, WF (reprint author), ARS, Environm Microbiol & Food Safety Lab, USDA, Bldg 303 Rm 011,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM walter.schmidt@ars.usda.gov NR 39 TC 3 Z9 3 U1 4 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0924-2031 EI 1873-3697 J9 VIB SPECTROSC JI Vib. Spectrosc. PD SEP PY 2015 VL 80 BP 59 EP 65 DI 10.1016/j.vibspec.2015.07.003 PG 7 WC Chemistry, Analytical; Chemistry, Physical; Spectroscopy SC Chemistry; Spectroscopy GA CT5OE UT WOS:000362858900010 ER PT J AU Dash, P Silwal, S Ikenga, JO Pinckney, JL Arslan, Z Lizotte, RE AF Dash, Padmanava Silwal, Saurav Ikenga, Julius O. Pinckney, James L. Arslan, Zikri Lizotte, Richard E. TI Water Quality of Four Major Lakes in Mississippi, USA: Impacts on Human and Aquatic Ecosystem Health SO WATER LA English DT Article DE harmful algal blooms; pathogens; toxic metals; cyanobacteria; microcystin; remote sensing ID PHYTOPLANKTON COMMUNITY STRUCTURE; CYANOBACTERIAL TOXINS; SPATIAL-DISTRIBUTION; TEMPORAL PATTERNS; CLASS ABUNDANCES; INLAND WATERS; PEARL RIVER; COASTAL; RESERVOIR; BASIN AB Harmful algal blooms (HABs), harmful microorganisms (pathogens) and toxic metals represent three major agents of water quality deterioration. Water quality of three northern lakes (Sardis, Enid, and Grenada) and a central lake (Ross Barnett Reservoir) of Mississippi, USA were examined in this study. While all these lakes are heavily used for recreational purposes, the Ross Barnett Reservoir serves additionally as the primary water supply for the City of Jackson, the capital city of Mississippi. The main goal of this study was to comprehensively assess the water quality of these lakes employing field and satellite data, and evaluate the potential human and aquatic health impacts. A time-series of true color images derived from satellite data indicated that algal blooms have been a recurring phenomenon in these lakes. Cyanobacteria, the algal group that predominantly occur in freshwater and form toxic blooms, were always present in these lakes and were most abundant on many occasions. The most toxic cyanotoxin, microcystin-LR, was found in all lakes, and its concentrations exceeded federal drinking water guidelines for children under six years of age many times. Potential bioaccumulation and biomagnification of microcystin-LR may pose serious risk to the aquatic ecosystem and human health including adults. Nutrient measurements indicated that all four lakes were eutrophic. Among bacterial populations, total coliforms and enterococci exceeded guideline values on several occasions. Arsenic, cadmium, chromium, and lead were found in the water of all the lakes, with arsenic exceeding the guideline values at two sites in Ross Barnett Reservoir. While it is apparent from this study that these lakes face many water quality issues, data across all seasons will be required to document potential trends and to devise management strategies. Use of remote sensing technology is recommended to monitor some of the water quality parameters such as suspended particulate matter and algal blooms, especially cyanobacterial blooms. C1 [Dash, Padmanava; Silwal, Saurav] Mississippi State Univ, Dept Geosci, Mississippi State, MS 39762 USA. [Ikenga, Julius O.] Mississippi Valley State Univ, Dept Nat Sci & Environm Hlth, Itta Bena, MS 38941 USA. [Pinckney, James L.] Univ S Carolina, Marine Sci Program, Columbia, SC 29208 USA. [Pinckney, James L.] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. [Arslan, Zikri] Jackson State Univ, Dept Chem & Biochem, Jackson, MS 39217 USA. [Lizotte, Richard E.] USDA ARS, Water Qual & Ecol Res Unit, Oxford, MS 38655 USA. RP Dash, P (reprint author), Mississippi State Univ, Dept Geosci, Mississippi State, MS 39762 USA. EM pd175@msstate.edu; ss2983@msstate.edu; jikenga@mvsu.edu; pinckney@sc.edu; zikri.arslan@jsums.edu; Richard.Lizotte@ars.usda.gov FU Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health [P20GM103476]; Faculty Start-up grant; Faculty Development Research grant FX The authors are indebted to the outstanding contributions of undergraduate student interns Rayford Parnell, LaTia Peavy, Michael Collins, Tasha Norwood, Grant Ikenga, Daniel Kibet, Winny Tanui, Joyce Chumo, and Marlon Flowers in Padmanava Dash's laboratory with summer internship support from Mississippi INBRE, funded by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103476. Field sampling costs were supported by the Faculty Start-up grant to Padmanava Dash and a Faculty Development Research grant to Julius O. Ikenga. The authors would like to thank the four anonymous reviewers for their timely review and helpful comments. NR 86 TC 0 Z9 0 U1 8 U2 50 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD SEP PY 2015 VL 7 IS 9 BP 4999 EP 5030 DI 10.3390/w7094999 PG 32 WC Water Resources SC Water Resources GA CT1MJ UT WOS:000362562200022 ER PT J AU Sicher, RC AF Sicher, Richard C. TI Temperature Shift Experiments Suggest That Metabolic Impairment and Enhanced Rates of Photorespiration Decrease Organic Acid Levels in Soybean Leaflets Exposed to Supra-Optimal Growth Temperatures SO METABOLITES LA English DT Article DE primary metabolism; TCA cycle; photosynthetic flux; CO2 enrichment; abiotic stress; leaf development ID HEAT-SHOCK PROTEINS; TCA CYCLE; CO2 ENRICHMENT; STRESS; PLANTS; LEAVES; RESPONSES; COMPLEX AB Elevated growth temperatures are known to affect foliar organic acid concentrations in various plant species. In the current study, citrate, malate, malonate, fumarate and succinate decreased 40 to 80% in soybean leaflets when plants were grown continuously in controlled environment chambers at 36/28 compared to 28/20 degrees C. Temperature effects on the above mentioned organic acids were partially reversed three days after plants were transferred among optimal and supra-optimal growth temperatures. In addition, CO2 enrichment increased foliar malate, malonate and fumarate concentrations in the supra-optimal temperature treatment, thereby mitigating effects of high temperature on respiratory metabolism. Glycerate, which functions in the photorespiratory pathway, decreased in response to CO2 enrichment at both growth temperatures. The above findings suggested that diminished levels of organic acids in soybean leaflets upon exposure to high growth temperatures were attributable to metabolic impairment and to changes of photorespiratory flux. Leaf development rates differed among temperature and CO2 treatments, which affected foliar organic acid levels. Additionally, we report that large decreases of foliar organic acids in response to elevated growth temperatures were observed in legume species. C1 USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. RP Sicher, RC (reprint author), USDA ARS, Crop Syst & Global Change Lab, Room 332,Bldg 001,BARC West 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM richard.sicher@ars.usda.gov NR 23 TC 3 Z9 4 U1 3 U2 8 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2218-1989 J9 METABOLITES JI Metabolites PD SEP PY 2015 VL 5 IS 3 BP 443 EP 454 DI 10.3390/metabo5030443 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CU0LO UT WOS:000363208200003 PM 26251925 ER PT J AU Zeng, HW Grapov, D Jackson, MI Fahrmann, J Fiehn, O Combs, GF AF Zeng, Huawei Grapov, Dmitry Jackson, Matthew I. Fahrmann, Johannes Fiehn, Oliver Combs, Gerald F., Jr. TI Integrating Multiple Analytical Datasets to Compare Metabolite Profiles of Mouse Colonic-Cecal Contents and Feces SO METABOLITES LA English DT Article DE metabolite; mass spectrometry; colon; cecal contents; feces ID MASS-SPECTROMETRY; METABOLOMICS; MICROBIOTA; BACTERIA; CANCER; HEALTH; GUT AB The pattern of metabolites produced by the gut microbiome comprises a phenotype indicative of the means by which that microbiome affects the gut. We characterized that phenotype in mice by conducting metabolomic analyses of the colonic-cecal contents, comparing that to the metabolite patterns of feces in order to determine the suitability of fecal specimens as proxies for assessing the metabolic impact of the gut microbiome. We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF). Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism. A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces. These data comprise the first characterization of relationships among metabolites present in the colonic-cecal contents and feces in a healthy mouse model, and shows that feces can be a useful proxy for assessing the pattern of metabolites to which the colonic mucosum is exposed. C1 [Zeng, Huawei; Jackson, Matthew I.; Combs, Gerald F., Jr.] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. [Grapov, Dmitry; Fahrmann, Johannes; Fiehn, Oliver] Univ Calif Davis, West Coast Metabol Ctr, Davis, CA 95616 USA. RP Zeng, HW (reprint author), ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. EM huawei.zeng@ars.usda.gov; dgrapov@ucdavis.edu; matthew.jackson@ars.usda.gov; jfahrmann@ucdavis.edu; ofiehn@ucdavis.edu; gerald.combs@ars.usda.gov NR 29 TC 2 Z9 2 U1 4 U2 14 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2218-1989 J9 METABOLITES JI Metabolites PD SEP PY 2015 VL 5 IS 3 BP 489 EP 501 DI 10.3390/metabo5030489 PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CU0LO UT WOS:000363208200006 PM 26378591 ER PT J AU Cardoza, RE McCormick, SP Malmierca, MG Olivera, ER Alexander, NJ Monte, E Gutierrez, S AF Cardoza, R. E. McCormick, S. P. Malmierca, M. G. Olivera, E. R. Alexander, N. J. Monte, E. Gutierrez, S. TI Effects of Trichothecene Production on the Plant Defense Response and Fungal Physiology: Overexpression of the Trichoderma arundinaceum tri4 Gene in T-harzianum SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID TUMOR-CELL LINES; TRICHODERMA-HARZIANUM; BOTRYTIS-CINEREA; FUSARIUM-SPOROTRICHIOIDES; SACCHAROMYCES-CEREVISIAE; SYSTEMIC RESISTANCE; BIOSYNTHESIS; TOMATO; EXPRESSION; SYNTHASE AB Trichothecenes are fungal sesquiterpenoid compounds, the majority of which have phytotoxic activity. They contaminate food and feed stocks, resulting in potential harm to animals and human beings. Trichoderma brevicompactum and T. arundinaceum produce trichodermin and harzianum A (HA), respectively, two trichothecenes that show different bioactive properties. Both compounds have remarkable antibiotic and cytotoxic activities, but in addition, trichodermin is highly phytotoxic, while HA lacks this activity when analyzed in vivo. Analysis of Fusarium trichothecene intermediates led to the conclusion that most of them, with the exception of the hydrocarbon precursor trichodiene (TD), have a detectable phytotoxic activity which is not directly related to the structural complexity of the intermediate. In the present work, the HA intermediate 12,13-epoxytrichothec-9-ene (EPT) was produced by expression of the T. arundinaceum tri4 gene in a transgenic T. harzianum strain that already produces TD after transformation with the T. arundinaceum tri5 gene. Purified EPT did not show antifungal or phytotoxic activity, while purified HA showed both antifungal and phytotoxic activities. However, the use of the transgenic T. harzianum tri4 strain induced a downregulation of defense-related genes in tomato plants and also downregulated plant genes involved in fungal root colonization. The production of EPT by the transgenic tri4 strain raised levels of erg1 expression and reduced squalene accumulation while not affecting levels of ergosterol. Together, these results indicate the complex interactions among trichothecene intermediates, fungal antagonists, and host plants. C1 [Cardoza, R. E.; Malmierca, M. G.; Gutierrez, S.] Univ Leon, Area Microbiol, Univ Sch Agr Engineers, Ponferrada, Spain. [McCormick, S. P.; Alexander, N. J.] USDA ARS, Bacterial Foodborne Pathogen & Mycol Unit, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Olivera, E. R.] Univ Leon, Area Biochem & Mol Biol, Ponferrada, Spain. [Monte, E.] Univ Salamanca, Spanish Portuguese Ctr Agr Res CIALE, Dept Genet & Microbiol, E-37008 Salamanca, Spain. RP Gutierrez, S (reprint author), Univ Leon, Area Microbiol, Univ Sch Agr Engineers, Campus Ponferrada, Ponferrada, Spain. EM s.gutierrez@unileon.es RI RODRIGUEZ OLIVERA, ELIAS/H-6385-2016; Gutierrez, Santiago/H-6456-2015; Monte, Enrique/A-9008-2017 OI RODRIGUEZ OLIVERA, ELIAS/0000-0003-2315-8524; Gutierrez, Santiago/0000-0001-6659-1390; Monte, Enrique/0000-0002-0166-5181 FU MICINN; MINECO [AGL2009-13431-C02, AGL2012-40041-C02-02]; Junta de Castilla y Leon [LE125A12-2]; FPU fellowship by the Spanish Ministry of Science and Innovation [AP2007-02835] FX This research was supported by grants from MICINN and MINECO (grants AGL2009-13431-C02 and AGL2012-40041-C02-02) and from the Junta de Castilla y Leon (grant LE125A12-2). M. G. Malmierca was granted an FPU fellowship by the Spanish Ministry of Science and Innovation (grant AP2007-02835). NR 44 TC 2 Z9 2 U1 0 U2 15 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD SEP PY 2015 VL 81 IS 18 BP 6355 EP 6366 DI 10.1128/AEM.01626-15 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CT2ZU UT WOS:000362676400026 PM 26150463 ER PT J AU Lingafelter, SW AF Lingafelter, Steven W. TI THE PRIONINAE (COLEOPTERA: CERAMBYCIDAE) OF HISPANIOLA, WITH DIAGNOSES, DESCRIPTIONS OF NEW SPECIES, DISTRIBUTION RECORDS, AND A KEY FOR IDENTIFICATION SO COLEOPTERISTS BULLETIN LA English DT Article DE Dominican Republic; Haiti; longhorned woodboring beetle; taxonomy; endemic species AB The Prioninae (Cerambycidae) of Hispaniola are reviewed. Twenty-eight species are known from the island, including six new species and a new genus described herein: Elateropsis dichroma Lingafelter, new species, Elateropsis woodleyi Lingafelter, new species, Solenoptera tomentosa Lingafelter, new species, Solenoptera helbi Lingafelter, new species, Solenoptera rugosa Lingafelter, new species, and Derancistrachroma melanoleuca Lingafelter, new genus and new species. Derancistrus furfurosus Galileo and Martins, 1993 is transferred to Solenoptera Audinet-Serville, 1832 as S. furfurosa, new combination; the holotype is determined to be a female, and the male is described for this species. Derancistrodes vittatus (Olivier, 1795) is transferred to Solenoptera as S. vittata, new combination, and Derancistrodes Galileo and Martins, 1993 is a new synonym of Solenoptera. New country records are recorded for Strongylaspis corticarius (Erichson, 1848), Monodesmus atratus Fisher, 1932, Elateropsis quinquenotatus Chevrolat, 1862, Elateropsis sericeiventris Chevrolat, 1862, and Elateropsis trimarginatus (Cazier and Lacey, 1952). The first known female of E. trimarginatus is described. Diagnoses and discussions are provided for each species. A key to all the Prioninae known from Hispaniola is included. C1 Natl Museum Nat Hist, USDA, Systemat Entomol Lab, Agr Res Serv, Washington, DC 20013 USA. RP Lingafelter, SW (reprint author), Natl Museum Nat Hist, USDA, Systemat Entomol Lab, Agr Res Serv, Washington, DC 20013 USA. NR 37 TC 2 Z9 2 U1 1 U2 7 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD SEP PY 2015 VL 69 IS 3 BP 353 EP 388 PG 36 WC Entomology SC Entomology GA CS8NA UT WOS:000362343500001 ER PT J AU Luan, JB Chen, WB Hasegawa, DK Simmons, AM Wintermantel, WM Ling, KS Fei, ZJ Liu, SS Douglas, AE AF Luan, Jun-Bo Chen, Wenbo Hasegawa, Daniel K. Simmons, Alvin M. Wintermantel, William M. Ling, Kai-Shu Fei, Zhangjun Liu, Shu-Sheng Douglas, Angela E. TI Metabolic Coevolution in the Bacterial Symbiosis of Whiteflies and Related Plant Sap-Feeding Insects SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE amino acid biosynthesis; bacteriocyte; Bemisia tabaci; Hamiltonella; horizontal gene transfer; Portiera ID HORIZONTAL GENE-TRANSFER; BEMISIA-TABACI; AMINO-ACIDS; RNA-SEQ; ENDOSYMBIOTIC BACTERIA; BUCHNERA-APHIDICOLA; TETRANYCHUS-URTICAE; GENOME SEQUENCE; EVOLUTION; EXPRESSION AB Genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. This study of the whitefly Bemisia tabaci and two bacteria (Portiera aleyrodidarum and Hamiltonella defensa) cohoused in each host cell investigated whether the decay of Portiera metabolism genes is complemented by host and Hamiltonella genes, and compared the metabolic traits of the whitefly symbiosis with other sap-feeding insects (aphids, psyllids, and mealybugs). Parallel genomic and transcriptomic analysis revealed that the host genome contributes multiple metabolic reactions that complement or duplicate Portiera function, and that Hamiltonella may contribute multiple cofactors and one essential amino acid, lysine. Homologs of the Bemisia metabolism genes of insect origin have also been implicated in essential amino acid synthesis in other sap-feeding insect hosts, indicative of parallel coevolution of shared metabolic pathways across multiple symbioses. Further metabolism genes coded in the Bemisia genome are of bacterial origin, but phylogenetically distinct from Portiera, Hamiltonella and horizontally transferred genes identified in other sap-feeding insects. Overall, 75% of the metabolism genes of bacterial origin are functionally unique to one symbiosis, indicating that the evolutionary history of metabolic integration in these symbioses is strongly contingent on the pattern of horizontally acquired genes. Our analysis, further, shows that bacteria with genomic decay enable host acquisition of complex metabolic pathways by multiple independent horizontal gene transfers from exogenous bacteria. Specifically, each horizontally acquired gene can function with other genes in the pathway coded by the symbiont, while facilitating the decay of the symbiont gene coding the same reaction. C1 [Luan, Jun-Bo; Douglas, Angela E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Chen, Wenbo; Hasegawa, Daniel K.; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Hasegawa, Daniel K.; Simmons, Alvin M.; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC USA. [Wintermantel, William M.] USDA ARS, Crop Improvement & Protect Res, Salinas, CA USA. [Fei, Zhangjun] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Liu, Shu-Sheng] Zhejiang Univ, Minist Agr, Key Lab Agr Entomol, Inst Insect Sci, Hangzhou, Zhejiang, Peoples R China. [Douglas, Angela E.] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA. RP Liu, SS (reprint author), Zhejiang Univ, Minist Agr, Key Lab Agr Entomol, Inst Insect Sci, Hangzhou, Zhejiang, Peoples R China. EM shshliu@zhu.edu.cn; aes326@cornell.edu OI Fei, Zhangjun/0000-0001-9684-1450 FU National Natural Science Foundation of China [31390421]; US Department of Agriculture, Agricultural Research Service [58-6659-3-123] FX The authors thank Gen-Hong Yan and Mihee Choi for assistance with plant growing; Dr Hua-ling Wang and Dr Xiangfeng Jing for assistance with the phylogenetic analysis; and Dr Qiong Rao, Dr Xuepeng Sun, and Professor Xiao-Wei Wang for assistance with RNA-Seq. This work was supported by the National Natural Science Foundation of China (31390421) and the US Department of Agriculture, Agricultural Research Service under Agreement No. 58-6659-3-123. NR 60 TC 16 Z9 16 U1 11 U2 55 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD SEP PY 2015 VL 7 IS 9 BP 2635 EP 2647 DI 10.1093/gbe/evv170 PG 13 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA CS9TB UT WOS:000362433600014 PM 26377567 ER PT J AU Brown, AMV Howe, DK Wasala, SK Peetz, AB Zasada, IA Denver, DR AF Brown, Amanda M. V. Howe, Dana K. Wasala, Sulochana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R. TI Comparative Genomics of a Plant-Parasitic Nematode Endosymbiont Suggest a Role in Nutritional Symbiosis SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE comparative genomics; nutritional symbiosis; Verrucomicrobia; Wolbachia; Akkermansia; Xiphinematobacter ID XIPHINEMA-AMERICANUM-GROUP; HORIZONTAL GENE-TRANSFER; BACTERIAL SYMBIONTS; SP-NOV.; AKKERMANSIA-MUCINIPHILA; INTRASPECIFIC VARIATION; PHYLUM VERRUCOMICROBIA; MICROBIAL SYMBIONTS; PURIFYING SELECTION; REDUCED GENOME AB Bacterial mutualists can modulate the biochemical capacity of animals. Highly coevolved nutritional mutualists do this by synthesizing nutrients missing from the host's diet. Genomics tools have advanced the study of these partnerships. Here we examined the endosymbiont Xiphinematobacter (phylum Verrucomicrobia) from the dagger nematode Xiphinema americanum, a migratory ectoparasite of numerous crops that also vectors nepovirus. Previously, this endosymbiont was identified in the gut, ovaries, and eggs, but its role was unknown. We explored the potential role of this symbiont using fluorescence in situ hybridization, genome sequencing, and comparative functional genomics. We report the first genome of an intracellular Verrucomicrobium and the first exclusively intracellular non-Woibachia nematode symbiont. Results revealed that Xiphinematobacter had a small 0.916-Mb genome with only 817 predicted proteins, resembling genomes of other mutualist endosymbionts. Compared with free-living relatives, conserved proteins were shorter on average, and there was large-scale loss of regulatory pathways. Despite massive gene loss, more genes were retained for biosynthesis of amino acids predicted to be essential to the host. Gene ontology enrichment tests showed enrichment for biosynthesis of arginine, histidine, and aromatic amino acids, as well as thiamine and coenzyme A, diverging from the profiles of relatives Akkermansia muciniphilia (in the human colon), Methylacidiphilum infemorum, and the mutualist Wolbachia from filarial nematodes. Together, these features and the location in the gut suggest that Xiphinematobacter functions as a nutritional mutualist, supplementing essential nutrients that are depleted in the nematode diet. This pattern points to evolutionary convergence with endosymbionts found in sap-feeding insects. C1 [Brown, Amanda M. V.; Howe, Dana K.; Wasala, Sulochana K.; Denver, Dee R.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Peetz, Amy B.; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. RP Brown, AMV (reprint author), Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. EM browaman@science.oregonstate.edu FU United States Department of Agriculture [SCA 58-5358-3-026] FX The authors thank Mark Dasenko at the Center for Genome Research and Bioinformatics (CGRB) at Oregon State University for sequencing assistance and Stephen Alderman and Mariella Ballato at the USDA-Agricultural Research Service for assisting with nematode collections. They also thank Wendy Phillips at the USDA for thoughtful input on the manuscript and Anne-Marie Girard at the Center for Genome Research and Biocomputing at Oregon State University for technical assistance with the confocal microscope. This work was supported by the United States Department of Agriculture (grant SCA 58-5358-3-026). NR 117 TC 5 Z9 5 U1 5 U2 23 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD SEP PY 2015 VL 7 IS 9 BP 2727 EP 2746 DI 10.1093/gbe/evv176 PG 20 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA CS9TB UT WOS:000362433600021 PM 26362082 ER PT J AU Wooton-Kee, CR Jain, AK Wagner, M Grusak, MA Finegold, MJ Lutsenko, S Moore, DD AF Wooton-Kee, Clavia Ruth Jain, Ajay K. Wagner, Martin Grusak, Michael A. Finegold, Milton J. Lutsenko, Svetlana Moore, David D. TI Elevated copper impairs hepatic nuclear receptor function in Wilson's disease SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID BILE-ACID HOMEOSTASIS; FARNESOID-X-RECEPTOR; CHOLESTEROL 7-ALPHA-HYDROXYLASE GENE; ACTIVATED-RECEPTOR; FACTOR 4-ALPHA; KNOCKOUT MICE; TRANSCRIPTIONAL REGULATION; TRANSPORTER GENES; DNA INTERACTIONS; P-GLYCOPROTEIN AB Wilson's disease (WD) is an autosomal recessive disorder that results in accumulation of copper in the liver as a consequence of mutations in the gene encoding the copper-transporting P-type ATPase (ATP7B). WD is a chronic liver disorder, and individuals with the disease present with a variety of complications, including steatosis, cholestasis, cirrhosis, and liver failure. Similar to patients with WD, Atp7b(-/-) mice have markedly elevated levels of hepatic copper and liver pathology. Previous studies have demonstrated that replacement of zinc in the DNA-binding domain of the estrogen receptor (ER) with copper disrupts specific binding to DNA response elements. Here, we found decreased binding of the nuclear receptors FXR, RXR, HNF4 alpha, and LRH-1 to promoter response elements and decreased mRNA expression of nuclear receptor target genes in Atp7b(-/-) Mice, as well as in adult and pediatric WD patients. Excessive hepatic copper has been described in progressive familial cholestasis (PFIC), and we found that similar to individuals with WD, patients with PFIC2 or PFIC3 who have clinically elevated hepatic copper levels exhibit impaired nuclear receptor activity. Together, these data demonstrate that copper-mediated nuclear receptor dysfunction disrupts liver function in WD and potentially in other disorders associated with increased hepatic copper levels. C1 [Wooton-Kee, Clavia Ruth; Moore, David D.] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Jain, Ajay K.] St Louis Univ, SSM Cardinal Glennon Childrens Med Ctr, Pediat Gastroenterol Hepatol & Nutr, St Louis, MO 63103 USA. [Wagner, Martin] Med Univ Graz, Dept Internal Med, Div Gastroenterol & Hepatol, Graz, Austria. [Grusak, Michael A.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. [Finegold, Milton J.] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA. [Lutsenko, Svetlana] Johns Hopkins Univ, Dept Physiol, Baltimore, MD USA. RP Moore, DD (reprint author), Baylor Coll Med, Dept Mol & Cellular Biol, One Baylor Plaza, Houston, TX 77030 USA. EM moore@bcm.edu FU NIH, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [5T32 DK-007664-19, F32-DK-089689-01A1, DK-56338]; R.P. Doherty, Jr.-Welch Chair in Science grant [Q-0022]; USDA-ARS [58-6250-6-003] FX This work was supported by grants from the NIH, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (5T32 DK-007664-19, F32-DK-089689-01A1, and DK-56338, to C.R. Wooton-Kee), which support the Texas Medical Center Digestive Diseases Center; an R.P. Doherty, Jr.-Welch Chair in Science grant (Q-0022, to D.D. Moore); and a USDA-ARS through Cooperative Agreement grant (58-6250-6-003, to M.A. Grusak). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 59 TC 8 Z9 8 U1 2 U2 11 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 EI 1558-8238 J9 J CLIN INVEST JI J. Clin. Invest. PD SEP PY 2015 VL 125 IS 9 BP 3449 EP 3460 DI 10.1172/JCI78991 PG 12 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA CS7YT UT WOS:000362303600022 PM 26241054 ER PT J AU Seo, EY Kim, HS Kim, JK Gotoh, T Hammond, J Lim, HS AF Seo, Eun-Young Kim, Hyun-Seung Kim, Jung-Kyu Gotoh, Takafumi Hammond, John Lim, Hyoun-Sub TI Utilization of a Tobacco Rattle Virus Vector to Clone a Nicotiana benthamiana cDNA Library for VIGS SO JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY LA English DT Article DE Tobacco rattle virus; N. benthamiana cDNA library; Virus induced gene silencing; Gateway cloning system; Reverse genetics ID GENE-FUNCTION; MOSAIC-VIRUS; GENOME SEQUENCE; PLANTS; EXPRESSION; MOVEMENT; PROTEINS; TOMATO AB Virus induced gene silencing (VIGS) is an efficient and rapid method to identify plant gene functions. One of the most widely used VIGS vectors is Tobacco rattle virus (TRV) which has been used successfully for RNA interference (RNAi) in N. benthamiana and tomato. We previously modified a TRV VIGS vector to contain the Gateway system for high throughput cloning (Ko et al., J. Fac.Agr.,Kyushu Univ., 60(1), 139-149 (2015)), and utilized this system to express a library of N. benthamiana cDNA. Random c.300 bp N. benthamiana cDNA fragments were generated by ultrasonication and inserted into the TRV VIGS vector by Gateway cloning. N benthamiana were agroinfiltrated with randomly selected TRV cDNA constructs in Agrobacterium tumefaciens GV 2260. Distinct visible phenotypes were identified in three sets of the inoculated N. benthamiana plants. The three distinguished phenotypes showed leaf malformation and necrosis. The three expressed gene inserts were homologous to EST fragments identified as CK290013.1, CK296346.1, and AM8112161.1, and presumably these genes are related to TRV pathogenesis in N. benthamiana. Identification of the selected genes by VIGS will aid further analysis to determine the relationship between VIGS phenotype and gene function. C1 [Seo, Eun-Young; Kim, Hyun-Seung; Kim, Jung-Kyu; Gotoh, Takafumi; Hammond, John; Lim, Hyoun-Sub] Kyushu Univ, Kuju Agr Res Ctr, Fac Agr, Oita 8780201, Japan. RP Hammond, J (reprint author), USDA ARS, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. EM john.hanunond@usda.ars.gov; hyounlim@cnu.ac.kr FU iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [112018-3] FX This research was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs (Project No. 112018-3), Republic of Korea. NR 20 TC 0 Z9 0 U1 1 U2 2 PU KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS PI FUKUOKA-SHI PA 6-10-1 HAKOZAKI, HIGASHI-KU, FUKUOKA-SHI, 812-0053, JAPAN SN 0023-6152 J9 J FAC AGR KYUSHU U JI J. Fac. Agric. Kyushu Univ. PD SEP PY 2015 VL 60 IS 2 BP 331 EP 337 PG 7 WC Agriculture, Multidisciplinary SC Agriculture GA CS9EA UT WOS:000362390700009 ER PT J AU Daimiel-Ruiz, L Klett-Mingo, M Konstantinidou, V Mico, V Aranda, JF Garcia, B Martinez-Botas, J Davalos, A Fernadez-Hernando, C Ordovas, JM AF Daimiel-Ruiz, Lidia Klett-Mingo, Mercedes Konstantinidou, Valentini Mico, Victor Aranda, Juan Francisco Garcia, Belen Martinez-Botas, Javier Davalos, Alberto Fernadez-Hernando, Carlos Maria Ordovas, Jose TI Dietary lipids modulate the expression of miR-107, a miRNA that regulates the circadian system (vol 59, pg 552, 2015) SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Correction DE Circadian rhythm; DHA; Dietary lipids; miR-107 ID CONJUGATED LINOLEIC-ACID; POLYUNSATURATED FATTY-ACIDS; CELL-CYCLE PROGRESSION; CARDIOVASCULAR-DISEASE; MICRORNA EXPRESSION; CACO-2 CELLS; FISH-OIL; CHOLESTEROL; METABOLISM; OBESITY AB Scope: The increased prevalence of cardiovascular diseases (CVDs) has been hypothesized to be the result of an increased exposure to a host of atherogenic environmental factors, paramount among them being unhealthy dietary habits. Long-chain n-3 polyunsaturated fatty acids (PUFAs) have been shown to have cardio protective effects, partially due to their ability to regulate gene expression. In this regard, increasing attention has been devoted to the role of miRNAs as regulators of multiple metabolic pathways whose deregulation has been associated with CVD risk. In this work we investigated whether miRNA expression was regulated by docosahexanoic acid, conjugated linoleic acid and cholesterol in Caco-2 cells. Results: Among the modulated miRNAs, miR-107 was differentially expressed by all treatments and this modulation was independent of its hosting gene, panthothenate kinase 1, possibly through its own promoter, which contains binding sites for metabolically relevant transcription factors. Among the putative target genes of miR-107, we found some genes with key roles in circadian rhythm. Specifically, we demonstrated that binding of miR-107 to the circadian locomotor output cycles kaput gene results in the deregulation of the circadian rhythm of the cells. Conclusion: Since chronodisruption has been linked to metabolic disorders such as type 2 diabetes, atherosclerosis, obesity, and CVD, our findings suggest that miR-107 could represent a new approach for pharmacological treatment of these diseases. C1 [Daimiel-Ruiz, Lidia; Klett-Mingo, Mercedes; Konstantinidou, Valentini; Mico, Victor; Garcia, Belen; Davalos, Alberto; Maria Ordovas, Jose] CEI UAM CSIC, IMDEA Food Inst, Nutr Genom Cardiovasc Dis & Obes, Madrid, Spain. [Aranda, Juan Francisco; Fernadez-Hernando, Carlos] Yale Univ, Sch Med, Vasc Biol & Therapeut Program, New Haven, CT USA. [Aranda, Juan Francisco; Fernadez-Hernando, Carlos] Yale Univ, Sch Med, Comparat Med Sect, Integrat Cell Signaling & Neurobiol Metab Program, New Haven, CT 06510 USA. [Martinez-Botas, Javier] Hosp Univ Ramon y Cajal, Inst Ramon y Cajal Invest Sanitaria, Serv Bioquim Invest, Madrid, Spain. [Maria Ordovas, Jose] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr, Boston, MA 02111 USA. RP Daimiel-Ruiz, L (reprint author), CEI UAM CSIC, IMDEA Food Inst, Nutr Genom Cardiovasc Dis & Obes, Madrid, Spain. EM Lidia.daimiel@imdea.org; jose.ordovas@tufts.edu NR 74 TC 1 Z9 1 U1 6 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD SEP PY 2015 VL 59 IS 9 BP 1865 EP 1878 DI 10.1002/mnfr.201570094 PG 14 WC Food Science & Technology SC Food Science & Technology GA CT1IG UT WOS:000362551400021 PM 26387852 ER PT J AU Sandmaier, SES Nandal, A Powell, A Garrett, W Blomberg, L Donovan, DM Talbot, N Telugu, BP AF Sandmaier, Shelley E. S. Nandal, Anjali Powell, Anne Garrett, Wesley Blomberg, Leann Donovan, David M. Talbot, Neil Telugu, Bhanu P. TI Generation of Induced Pluripotent Stem Cells From Domestic Goats SO MOLECULAR REPRODUCTION AND DEVELOPMENT LA English DT Article ID SOMATIC-CELLS; STATISTICAL-MODEL; NUCLEAR TRANSFER; RAT BLASTOCYSTS; MOUSE; LINES; FIBROBLASTS; PORCINE; EMBRYOS; CULTURE AB The creation of genetically modified goats provides a powerful approach for improving animal health, enhancing production traits, animal pharming, and for ensuring food safety all of which are high-priority goals for animal agriculture. The availability of goat embryonic stem cells (ESCs) that are characteristically immortal in culture would be of enormous benefit for developing genetically modified animals. As an alternative to long-sought goat ESCs, we generated induced pluripotent stem cells (iPSC) by forced expression of bovine POU5F1, SOX2, MYC, KLF4, LIN-28, and NANOG reprogramming factors in combination with a MIR302/367 cluster, delivered by lentiviral vectors. In order to minimize integrations, the reprogramming factor coding sequences were assembled with porcine teschovirus-1 2A (P2A) self-cleaving peptides that allowed for tri-cistronic expression fromeach vector. The lentiviral-transduced cells were cultured on irradiated mouse feeder cells in a semi-defined, serum-free medium containing fibroblast growth factor(FGF) and/or leukemia inhibitory factor (LIF). The resulting goat iPSC exhibit cell and colony morphology typical of human and mouse ESCs-that is, well-defined borders, a high nuclear-to-cytoplasmic ratio, a short cell-cycle interval, alkaline phosphatase expression, and the ability to generate teratomas in vivo. Additionally, these goat iPSC demonstrated the ability to differentiate into directed lineages in vitro. These results constitute the first steps in establishing integration and footprint-free iPSC from ruminants. C1 [Sandmaier, Shelley E. S.; Nandal, Anjali; Powell, Anne; Garrett, Wesley; Blomberg, Leann; Donovan, David M.; Talbot, Neil; Telugu, Bhanu P.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD USA. [Sandmaier, Shelley E. S.; Nandal, Anjali; Telugu, Bhanu P.] Univ Maryland, Anim & Avian Sci, College Pk, MD 20742 USA. RP Telugu, BP (reprint author), Univ Maryland, 2121 ANSC Bldg, College Pk, MD 20742 USA. EM btelugu@umd.edu FU Maryland Agriculture Experimental Station (MAES) Seed Grant; Maryland Stem Cell Research Fund (MSCRF) Exploratory Grant; Investigator funds from the Department of Animal and Avian Sciences, University of Maryland; BARD [CRIS 1245-31000-103-00D] FX Grant sponsor: Maryland Agriculture Experimental Station (MAES) Seed Grant; Grant sponsor: Maryland Stem Cell Research Fund (MSCRF) Exploratory Grant; Grant sponsor: Investigator funds from the Department of Animal and Avian Sciences, University of Maryland; Grant sponsor: BARD; Grant number: CRIS 1245-31000-103-00D NR 57 TC 2 Z9 4 U1 1 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1040-452X EI 1098-2795 J9 MOL REPROD DEV JI Mol. Reprod. Dev. PD SEP PY 2015 VL 82 IS 9 BP 709 EP 721 DI 10.1002/mrd.22512 PG 13 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Reproductive Biology SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Reproductive Biology GA CT1VH UT WOS:000362589800010 PM 26118622 ER PT J AU Guo, QF AF Guo, Qinfeng TI No consistent small-scale native-exotic relationships SO PLANT ECOLOGY LA English DT Article DE Community variables; Degree of invasion (DI); Invasibility; Niche theory; Richness; Space ID DIVERSITY REDUCES INVASIBILITY; SPECIES RICHNESS RELATIONSHIPS; MIXED-GRASS PRAIRIE; PLANT INVASIONS; SPATIAL SCALES; BIOLOGICAL INVASION; COMMUNITIES; DISTURBANCE; RESISTANCE; VEGETATION AB The perception that native and exotic species are negatively related to each other at small scales has proliferated in the ecological literature. Although mounting evidence shows that such a perception is not always reality, the degree to which the mismatch occurs and the causes are not clear. Here, I compile and synthesize data based on the smallest scales used in 75 case studies in plant communities around the world, and analyze detailed data from a study in early successional California chaparral. I show that (1) different metrics (community variables) yielded different results, (2) native biomass and cover had much stronger negative effects on exotic richness than native richness and density, (3) there is no consistent correlation between native and exotic richness, especially in natural and immature communities, and (4) proportionally more experimental studies revealed negative relations than field observations. Collectively, these results reveal and confirm a high degree of mismatch between perceptions and reality in small-scale patterns of biotic invasions which have management implications. C1 USDA, Southern Res Stn, Res Triangle Pk, NC 27709 USA. RP Guo, QF (reprint author), USDA, Southern Res Stn, 3041 Cornwallis Rd, Res Triangle Pk, NC 27709 USA. EM qguo@fs.fed.us NR 37 TC 1 Z9 1 U1 4 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD SEP PY 2015 VL 216 IS 9 BP 1225 EP 1230 DI 10.1007/s11258-015-0503-7 PG 6 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA CT3YO UT WOS:000362743000003 ER PT J AU Hay-Roe, MM Nagoshi, RN Meagher, RL De Lopez, MA Trabanino, R AF Hay-Roe, Mirian M. Nagoshi, Rodney N. Meagher, Robert L. De Lopez, Myriam Arias Trabanino, Rogelio TI Isolation and DNA Barcode Characterization of a Permanent Telenomus (Hymenoptera: Platygastridae) Population in Florida That Targets Fall Armyworm (Lepidoptera: Noctuidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Spodoptera frugiperda; parasitoid; biological control ID BIOLOGICAL-CONTROL; EGGS AB Telenomus remus Nixon is a platygastrid egg parasite of the fall armyworm, Spodoptera frugiperda (J. E. Smith), with a history of use as an augmentative biological control agent in Central and South America. Efforts were made in 1975-1977 and again in 1988-1989 to introduce T. remus into the fall armyworm overwintering regions of southern Florida to mitigate infestations by this migratory pest, but in neither case was evidence of long-term establishment found. However, in 2009 and again in 2013, an unidentified Telenomus species was found attacking fall armyworm sentinel egg masses placed on corn plants or pasture grasses in the north-central Florida counties of Levy and Alachua. Taxonomic uncertainties have so far not allowed a conclusive identification of species by morphological keys. DNA barcode comparisons showed a single Florida haplotype in all collections that was identical to that found in a T. remus colony from Ecuador and very similar to a T. remus colony from Honduras. The T. remus barcode sequences were phylogenetically distinct from a second Telenomus species from Ecuador, T. rowani, and from other related sequences obtained from the NCBI GenBank database. This represents the first observation of a permanent Telenomus population in the United States that targets fall armyworm and provides genetic evidence for its identification as T. remus. These findings have positive implications for the use of augmentative biological control methods to mitigate fall armyworm migration from Florida. C1 [Hay-Roe, Mirian M.; Nagoshi, Rodney N.; Meagher, Robert L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Hay-Roe, Mirian M.] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [De Lopez, Myriam Arias] Inst Nacl Autonomo Invest Agr, Sede Estn Expt Litoral Sur, Guayas, Ecuador. [Trabanino, Rogelio] Zamorano, Tegucigalpa, Honduras. RP Nagoshi, RN (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM rodney.nagoshi@ars.usda.gov FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-67003-30209] FX We acknowledge the assistance of Matthew Buffington, Systematics Entomology Laboratory, ARS, USDA, Washington, DC, in identifying Telenomus sp., to A. Rowley and N. Fieleke for field assistance and insect colony maintenance. We thank Jean Thomas at USDA-ARS for excellent technical support and assistance. We are grateful to Dr. Sandra Allan (USDA-ARS) and Dr. Steven Valles (USDA-ARS) for helpful comments on the manuscript. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. This material is based upon [work] supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Agreement No. 2011-67003-30209. NR 23 TC 0 Z9 0 U1 2 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD SEP PY 2015 VL 108 IS 5 BP 729 EP 735 DI 10.1093/aesa/sav074 PG 7 WC Entomology SC Entomology GA CS4JK UT WOS:000362041900009 ER PT J AU Zagvazdina, NY Paris, TM Udell, BJ Stanislauskas, M McNeill, S Allan, SA Mankin, RW AF Zagvazdina, N. Y. Paris, T. M. Udell, B. J. Stanislauskas, M. McNeill, S. Allan, S. A. Mankin, R. W. TI Effects of Atmospheric Pressure Trends on Calling, Mate-Seeking, and Phototaxis of Diaphorina citri (Hemiptera: Liviidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Acoustics; vibration; psyllid; citrus; weather ID BAROMETRIC-PRESSURE; VIBRATIONAL COMMUNICATION; METEOROLOGICAL FACTORS; INSECT DETECTION; PSYLLIDAE; BEHAVIOR; RESPONSES; FLIGHT; OVIPOSITION; ORIENTATION AB Insects and other animals sometimes modify behavior in response to changes in atmospheric pressure, an environmental cue that can provide warning of potentially injurious windy and rainy weather. To determine if Diaphorina citri ( Hemiptera: Liviidae) calling, mate- seeking, and phototaxis behaviors were affected by atmospheric pressure, we conducted analyses to correlate responsiveness with pressure trends over periods up to 48 h before laboratory bioassays. Mean responsiveness increased or decreased depending on the magnitudes and directions of pressure changes measured over different periods up to 24 h before bioassays, and changed differently in calling and mate- seeking bioassays than in phototaxis bioassays. For example, mean responsiveness decreased in mating behavior bioassays but increased in phototaxis bioassays when atmospheric pressure changed more than one standard deviation over a 24-h period. Such a result is consistent with a hypothesis that there may be survival benefits to focusing energy on dispersal or migration rather than mating after occurrences of sustained, unusual pressure changes. A finding that mean phototactic responses increased when pressure decreased over 9-24- h periods before bioassays is potentially of practical interest. More knowledge about the effects of atmospheric pressure and other environmental variables on behavior can lead to improved models of psyllid movement or other pest management tools as well as to improved timing of application of pest management tools. C1 [Zagvazdina, N. Y.; Udell, B. J.; Stanislauskas, M.; Allan, S. A.; Mankin, R. W.] ARS, USDA, CMAVE, Gainesville, FL 32608 USA. [Paris, T. M.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [McNeill, S.] Union Coll, Div Sci & Math, Lincoln, NE 68506 USA. RP Mankin, RW (reprint author), ARS, USDA, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM Richard.Mankin@ars.usda.gov FU Citrus Research and Development Foundation FX We thank Betty Weaver, Heidi Burnside, and Everett Foreman (USDA-ARS-CMAVE) for rearing assistance and technical consultation. Funding was provided in part by a grant from the Citrus Research and Development Foundation. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. The USDA is an equal-opportunity employer. NR 53 TC 3 Z9 3 U1 3 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD SEP PY 2015 VL 108 IS 5 BP 762 EP 770 DI 10.1093/aesa/sav069 PG 9 WC Entomology SC Entomology GA CS4JK UT WOS:000362041900012 ER PT J AU Favret, C Tzaud, M Erbe, EF Bauchan, GR Ochoa, R AF Favret, Colin Tzaud, Matthieu Erbe, Eric F. Bauchan, Gary R. Ochoa, Ron TI An Adhesive Collophore May Help Direct the Springtail Jump SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Collembola; low temperature scanning electron microscopy (LT-SEM); ventral tube; furcula; Entomobrya multifasciata ID CERATOPHYSELLA-SIGILLATA COLLEMBOLA; TRANSPORTING EPITHELIUM; VENTRAL TUBE; HYPOGASTRURIDAE; MORPHOLOGY; BEHAVIOR; INSECTA AB The collophore of Entomobrya multifasciata ( Tullberg) ( Collembola: Entomobryidae) is composed of four segments. The third segment telescopes in and out of the second and the fourth is an eversible vesicle that is entirely enclosed in the third when not deployed. Low temperature scanning electron micrographs document that the collophore may remain adhered to the substrate even as the springtail initiates its jump. This adherence appears to force the posterior abdomen into the air, causing the jump itself to be initiated in a forward direction and accompanied by a forward flip. Among the several other documented functions of the collophore, we suggest that it may serve to affect the direction and trajectory of the springtail during its jump. C1 [Favret, Colin; Tzaud, Matthieu] Univ Montreal, Biodivers Ctr, Dept Biol Sci, Montreal, PQ H1X 2B2, Canada. [Erbe, Eric F.; Bauchan, Gary R.] USDA ARS, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA. [Ochoa, Ron] USDA ARS, Systemat Entomol Lab, Beltsville, MD 20705 USA. RP Favret, C (reprint author), Univ Montreal, Biodivers Ctr, Dept Biol Sci, 4101 Rue Sherbrooke Est, Montreal, PQ H1X 2B2, Canada. EM ColinFavret@AphidNet.org RI Favret, Colin/F-7726-2012 OI Favret, Colin/0000-0001-6243-3184 NR 27 TC 0 Z9 0 U1 4 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD SEP PY 2015 VL 108 IS 5 BP 814 EP 819 DI 10.1093/aesa/sav078 PG 6 WC Entomology SC Entomology GA CS4JK UT WOS:000362041900018 ER PT J AU Vidovic, B Cvrkovic, T Maric, I Chetverikov, PE Cristofaro, M Rector, BG Petanovic, R AF Vidovic, Biljana Cvrkovic, Tatjana Maric, Ivana Chetverikov, Philipp E. Cristofaro, Massimo Rector, Brian G. Petanovic, Radmila TI A New Metaculus Species (Acari: Eriophyoidea) on Diplotaxis tenuifolia (Brassicaceae) From Serbia: A Combined Description Using Morphology and DNA Barcode Data SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE weed biological control; Eriophyidae; mite; morphological trait; MT-CO1 ID WHEAT CURL MITE; BIOLOGICAL-CONTROL; KEIFER ACARI; HOST; SPECIALIZATION; MITOCHONDRIAL; ERIOPHYIDAE; SYSTEMATICS; PROSTIGMATA; POPULATIONS AB A new species of eriophyoid mite, Metaculus diplotaxi n. sp. inhabiting Diplotaxis tenuifolia ( L.) DC., is described from Serbia. To investigate interspecific variability between Metaculus spp. on three different Brassicaceae host plants ( viz. D. tenuifolia, Lepidium latifolium ( L.), Isatis tinctoria ( L.)), we analyzed phenotypic variability of morphological traits and molecular sequences of the mitochondrial cytochrome oxidase subunit I ( MT- CO1). Discriminant analysis identified seven traits that significantly differentiate three Metaculus spp: M. lepidifolii, M. rapistri, and M. diplotaxi n. sp. Analysis of MT- CO1 sequences supported the results obtained from the analysis of morphometric features. C1 [Vidovic, Biljana; Petanovic, Radmila] Univ Belgrade, Fac Agr, Dept Entomol & Agr Zool, Belgrade 11080, Serbia. [Cvrkovic, Tatjana] Inst Plant Protect & Environm, Belgrade 11080, Serbia. [Maric, Ivana] Inst Pesticides & Environm Protect, Belgrade 11080, Serbia. [Chetverikov, Philipp E.] Russian Acad Sci, Inst Zool, St Petersburg 199034, Russia. [Chetverikov, Philipp E.] St Petersburg State Univ, Dept Invertebrate Zool, St Petersburg 199034, Russia. [Cristofaro, Massimo] UTAGRI ECO, ENEA Casaccia, I-00123 Rome, Italy. [Rector, Brian G.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. RP Rector, BG (reprint author), USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. EM brian.rector@ars.usda.gov RI Chetverikov, Philipp/H-3729-2013; OI Chetverikov, Philipp/0000-0003-1300-1486; Cvrkovic, Tatjana/0000-0001-5108-2323 FU Serbian Ministry of Science and Environment Protection [III 43001]; Russian Science Foundation (RSCF grant) [14-14-00621]; Saint-Petersburg State University [1.42.1289.2014] FX We are grateful to Dr. Vladimir Stevanovic, Faculty of Biology, University of Belgrade, for the identification of plant species; Mrs. Franca di Christina, BBCA ONLUS, Italy, for collecting the mites; and Mrs. Dragica Smiljanic for collecting mites and creating the technical illustrations. We would also like to thank the two referees for their helpful suggestions in a preliminary draft. The study was supported by the Serbian Ministry of Science and Environment Protection (Grant III 43001). CLSM study of mites was supported by Russian Science Foundation (RSCF grant 14-14-00621) to P.C. Travel by P.C. was supported by Saint-Petersburg State University (Grant 1.42.1289.2014). NR 53 TC 0 Z9 0 U1 2 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD SEP PY 2015 VL 108 IS 5 BP 922 EP 931 DI 10.1093/aesa/sav076 PG 10 WC Entomology SC Entomology GA CS4JK UT WOS:000362041900028 ER PT J AU Hansen, JA Moulton, JK Klingeman, WE Oliver, JB Windham, MT Trigiano, RN Reding, ME AF Hansen, Jason A. Moulton, John K. Klingeman, William E. Oliver, Jason B. Windham, Mark T. Trigiano, Robert N. Reding, Michael E. TI Molecular Systematics of the Chrysobothris femorata Species Group (Coleoptera: Buprestidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE femorata complex; flatheaded appletree borer; jewel beetle; metallic woodboring beetle ID MITOCHONDRIAL-DNA; PHYLOGENETIC UTILITY; BEETLES COLEOPTERA; MAXIMUM-LIKELIHOOD; NUCLEAR GENES; MIXED MODELS; NORTH; DIPTERA; BORER; ATTRACTION AB The Chrysobothris femorata ( Olivier) species group has expanded to 12 recognized species. Many of these species are sympatric, share larval host plants, and are difficult to reliably separate morphologically. This study investigated species limits and relationships among the recognized species occurring within the southeastern United States. Results are presented from phylogenetic analyses including several group members using sequences from cytochrome oxidase I ( cox I) and arginine kinase ( AK). Cox I analyses validated the monophyly of several Chrysobothris species outside of the C. femorata species group and recovered group members C. adelpha Harold, C. viridiceps Melsheimer, and C. wintu Wellso and Manley as monophyletic. However, cox I analyses yielded well supported nodes that rendered C. femorata Olivier, C. quadriimpressa Gory and Laporte, C. rugosiceps Melsheimer, and C. shawnee Wellso and Manley paraphyletic. AK sequences provided statistical support for monophyly of C. wintu and two well supported clades that rendered C. femorata and C. quadriimpressa paraphyletic. Imperfect taxonomy cannot totally account for this phenomenon. The observed pattern could be the result of ancestral polymorphism or lineage sorting, yet introgression of these C. femorata species group members is possible. Although observed results from these genes suggest potential taxonomic synonymization, such steps are premature. Greater understanding of interactions between group members as occurs within their overlapping geographies and host plants is needed. We suggest future molecular studies adopting a population genetics approach utilizing several nuclear genes or SNiPs acquired subsequent to a NextGen sequencing campaign. C1 [Hansen, Jason A.; Moulton, John K.; Windham, Mark T.; Trigiano, Robert N.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Klingeman, William E.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Oliver, Jason B.] TSU, Otis Floyd Nursery Crops Res Stn, McMinnville, TN 37110 USA. [Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, ATRU,Hort Insects Lab, Wooster, OH 44691 USA. RP Moulton, JK (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, 2431 Joe Johnson Dr, Knoxville, TN 37996 USA. EM jmoulton@utk.edu FU USDA-ARS [58-6404-7-213] FX We are grateful to Stan Wellso for generously identifying specimens from the Chrysobothris femorata species group. We would also like to thank Richard Westcott for providing specimens and Joshua Basham and four anonymous reviewers for constructive comments upon earlier drafts that significantly improved the manuscript. This study was supported by funding from USDA-ARS (58-6404-7-213). NR 66 TC 0 Z9 0 U1 4 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD SEP PY 2015 VL 108 IS 5 BP 950 EP 963 DI 10.1093/aesa/sav080 PG 14 WC Entomology SC Entomology GA CS4JK UT WOS:000362041900031 ER PT J AU Jewitt, D Erasmus, BFN Goodman, PS O'Connor, TG Hargrove, WW Maddalena, DM Witkowski, ETF AF Jewitt, Debbie Erasmus, Barend F. N. Goodman, Peter S. O'Connor, Timothy G. Hargrove, William W. Maddalena, Damian M. Witkowski, Ed. T. F. TI Climate-induced change of environmentally defined floristic domains: A conservation based vulnerability framework SO APPLIED GEOGRAPHY LA English DT Article DE Climatic stability; Conservation planning; Gradient analysis; Habitat intactness; Refugia ID BIODIVERSITY CONSERVATION; ADAPTATION STRATEGIES; SOUTHERN AFRICA; CHANGE IMPACTS; DIVERSITY; MANAGEMENT; ECOLOGY; DEBATE; FOREST; MODEL AB Global climate change is having marked influences on species distributions, phenology and ecosystem composition and raises questions as to the effectiveness of current conservation strategies. Conservation planning has only recently begun to adequately account for dynamic threats such as climate change. We propose a method to incorporate climate-dynamic environmental domains, identified using specific environmental correlates of floristic composition, into conservation strategies, using the province of KwaZulu-Natal, South Africa as a case study. The environmental domains offer an approach to conservation that conserves diversity under current and future climates, recognising that the species constituting diversity may change through time. We mapped current locations of domains by identifying their positions in a multi-dimensional environmental space using a non-hierarchical iterative k-means clustering algorithm. Their future locations were explored using an ensemble of future climate scenarios. The HadCM2 and GFDL2.1 models represented the extreme ranges of the models. The magnitude of change in each environmental domain was calculated using Euclidean distances to determine areas of greatest and least stability for each future climate projection. Domains occurring in the savanna biome increase at the expense of domains occurring in the grassland biome, which has significant negative consequences for the species rich grasslands. The magnitude of change maps represents areas of changed climatic conditions or edaphic disjunctions. The HadCM2 model predicted the greatest overall magnitude of change across the province. Species with specific soil requirements may not be able to track changing climatic conditions. A vulnerability framework was developed that incorporated climatic stability and habitat intactness indices. The mean magnitude of change informed the potential speed of transition of domains between the vulnerability quadrants. The framework informs appropriate conservation actions to mitigate climate change impacts on biodiversity. The study explicitly links floristic pattern and climate variability and provides useful insights to facilitate conservation planning for climate change. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Jewitt, Debbie; Goodman, Peter S.] Ezemvelo KZN Wildlife, Biodivers Div, Sci Serv, ZA-3202 Cascades, South Africa. [Jewitt, Debbie; Erasmus, Barend F. N.; Goodman, Peter S.; O'Connor, Timothy G.; Witkowski, Ed. T. F.] Univ Witwatersrand, Sch Anim Plant & Environm Sci, ZA-2050 Johannesburg, South Africa. [O'Connor, Timothy G.] SAEON, ZA-0001 Pretoria, South Africa. [Hargrove, William W.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Asheville, NC 28804 USA. [Maddalena, Damian M.] Univ N Carolina, Dept Environm Studies, Wilmington, NC 28401 USA. RP Jewitt, D (reprint author), Ezemvelo KZN Wildlife, Biodivers Div, Sci Serv, POB 13053, ZA-3202 Cascades, South Africa. EM Debbie.Jewitt@kznwildlife.com; Barend.Erasmus@wits.ac.za; pgoodman@conservation-solutions.org; tim@saeon.ac.za; hnw@geobabble.org; maddalenad@uncw.edu; Ed.Witkowski@wits.ac.za FU Society for Conservation GIS; ESRI; Ezemvelo KZN Wildlife FX The Society for Conservation GIS and ESRI are thanked for their scholarship and software grant. Ezemvelo KZN Wildlife is thanked for their study bursary. NR 46 TC 3 Z9 4 U1 4 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0143-6228 EI 1873-7730 J9 APPL GEOGR JI Appl. Geogr. PD SEP PY 2015 VL 63 BP 33 EP 42 DI 10.1016/j.apgeog.2015.06.004 PG 10 WC Geography SC Geography GA CS4PZ UT WOS:000362059700004 ER PT J AU Allen, C Valles, SM AF Allen, Clare Valles, Steven M. TI Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system SO FLORIDA ENTOMOLOGIST LA English DT Article DE Solenopsis invicta; unassigned virus; expression; biological control ID IMPORTED FIRE ANT; STRAND RNA VIRUS; HYMENOPTERA-FORMICIDAE; SUCCESSFUL TRANSMISSION; NORTH-AMERICA; SPECIFICITY; COLONIES AB Solenopsis invicta virus 3 (SINV-3) is an unclassified positive stranded RNA virus whose characteristics are amenable to development as a microbial insecticide or as a classical biological control agent for the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae). Bait formulations containing SINV-3 have been produced and were used successfully to transmit the infection and cause significant mortality to fire ant colonies in the laboratory. Unfortunately, there is no available means of propagating infectious SINV-3 particles in vitro, and this has hampered its evaluation and development as a microbial insecticide. In this study, we attempted to utilize a baculovirus expression system as reported by others to produce SINV-3 infectious particles in quantity. A full-length copy of the SINV-3 genome was assembled successfully from 3 overlapping fragments, and a heterologous bacmid-SINV-3 vector (BAC_SINV-3) was produced. The insect cell line Sf21 was transfected with purified high-molecular weight BAC_SINV-3 and supported production of baculovirus expressing full-length SINV-3 transcripts. Despite successful transcription of the SINV-3 genome, evidence that translation was occurring could not be produced. Western analyses for the SINV-3 VP-2 capsid protein were consistently negative. C1 [Allen, Clare; Valles, Steven M.] ARS, Ctr Med Agr & Vet, USDA, Gainesville, FL 32608 USA. RP Valles, SM (reprint author), ARS, Ctr Med Agr & Vet, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov FU USDA FX C. Allen was supported by the USDA research associate program, class of 2009. We thank R. Nagoshi and A. Estep (USDA-ARS, Gainesville, Florida, USA) for critical reviews of the manuscript, C. Strong (US-DA-ARS) for technical assistance, and B. Bonning (Iowa State University, Ames, Iowa, USA) for providing the AcRP23. lacZ. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 20 TC 0 Z9 0 U1 0 U2 1 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD SEP PY 2015 VL 98 IS 3 PG 7 WC Entomology SC Entomology GA CS2QC UT WOS:000361915000020 ER PT J AU Brar, GS Capinera, JL Kendra, PE Smith, JA Pena, JE AF Brar, Gurpreet S. Capinera, John L. Kendra, Paul E. Smith, Jason A. Pena, Jorge E. TI Temperature-dependent development of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE avocado; redbay; temperature; development models ID REDBAY AMBROSIA BEETLE; LIGNOSELLUS LEPIDOPTERA-PYRALIDAE; SOUTHEASTERN UNITED-STATES; CODLING MOTH LEPIDOPTERA; LAUREL WILT; RAFFAELEA-LAURICOLA; CONSTANT TEMPERATURES; DEVELOPMENT TIME; LIFE-CYCLE; 1ST REPORT AB Redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae), is a nonnative pest that transmits the pathogenic fungus Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva (Ophiostomatales: Ophiostomataceae), which causes laurel wilt disease in trees of the family Lauraceae. Laurel wilt is present in the commercial avocado (Persea americana Mill.; Laurales: Lauraceae) growing areas of Florida and poses a potential threat to the avocado industries of California and Mexico. The life cycle of X. glabratus was studied in avocado logs at 16, 20, 24, 28, and 32 degrees C. Xyleborus glabratus successfully completed its life cycle at 24, 28, and 32 degrees C, with the greatest oviposition and development rate at 28 degrees C. Development of the egg and pupal stages was studied at 12, 16, 18, 20, 24, 28, 32, and 36 degrees C. One linear and 7 nonlinear developmental models were used to estimate the temperature-dependent development of both stages. The linear model estimated the lower threshold temperatures for egg and pupal development to be 13.8 degrees C and 11.1 degrees C, respectively, and the degree-days (DD) for egg and pupal development to be 55.1 DD and 68.2 DD, respectively. The Brier-2, Ratkowsky, Logan, and polynomial models gave the best estimates for the temperature-dependent development of the egg stages, whereas the Brier-1, Logan, and polynomial models gave the best estimates of temperature-dependent development of the pupal stages. Our results suggested that the optimal temperature for development of X. glabratus was around 28 degrees C, and that temperature will play an important role in the spread and successful establishment of X. glabratus. C1 [Brar, Gurpreet S.] Univ Florida, IFAS, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [Capinera, John L.] Univ Florida, IFAS, Entomol & Nematol Dept, Gainesville, FL 32611 USA. [Kendra, Paul E.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Smith, Jason A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Pena, Jorge E.] Univ Florida, IFAS, Trop Res & Educ Ctr, Homestead, FL 33031 USA. RP Brar, GS (reprint author), Univ Florida, IFAS, Everglades Res & Educ Ctr, 3200 E Palm Beach Rd, Belle Glade, FL 33430 USA. EM gpsbrar@ufl.edu FU SCRI grant FX We gratefully acknowledge Stephen McLean (Entomology and Nematology Department, University of Florida) for help in maintaining the redbay ambrosia beetle colony. We also gratefully acknowledge the Hammock Dunes Club Golf Course, Palm Bay (Brevard County) for allowing us to cut infested redbay and swampbay wood. We gratefully acknowledge the assistance of James Colee (IFAS Statistics, University of Florida). This research was supported by a SCRI grant to Dr. R. C. Ploetz (University of Florida, TREC, Homestead, Florida). NR 50 TC 0 Z9 0 U1 4 U2 13 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD SEP PY 2015 VL 98 IS 3 PG 9 WC Entomology SC Entomology GA CS2QC UT WOS:000361915000006 ER PT J AU Pena, JE Weihman, SW McLean, S Cave, RD Carrillo, D Duncan, RE Evans, G Krauth, S Thomas, MC Lu, SS Kendra, PE Roda, AL AF Pena, Jorge E. Weihman, Scott W. McLean, Stephen Cave, Ronald D. Carrillo, Daniel Duncan, Rita E. Evans, Gregory Krauth, Stephen Thomas, M. C. Lu, S. S. Kendra, Paul E. Roda, Amy L. TI Predators and parasitoids associated with Scolytinae in Persea species (Laurales: Lauraceae) and other Lauraceae in Florida and Taiwan SO FLORIDA ENTOMOLOGIST LA English DT Article DE red bay ambrosia beetle; predator; parasitoids; avocado ID REDBAY AMBROSIA BEETLE; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; SOUTHEASTERN UNITED-STATES; COFFEE BERRY BORER; XYLEBORUS-GLABRATUS; WILT; SYMBIONT; VECTOR; MANUKA; LURES AB The redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae), due to its association with Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva (Ophiostomatales: Ophiostomataceae), a pathogen that causes laurel wilt, is considered one of the most damaging pests of Persea species (Laurales: Lauraceae) including avocado. Currently, there is no satisfactory method to control this pest. Biological control is being examined as an additional tool to be used to lower the pest population and slow its spread. The objective of this study was to determine the natural enemy community associated with X. glabratus in Florida and Taiwan by using 3 methods: 1) field-collected wood naturally infested with X. glabratus (Florida), 2) bolts of avocado artificially infested with X. glabratus (Florida) and 3) direct collection of natural enemies from a trap area baited with infested wood and known X. glabratus lures (Florida and Taiwan). Among the predacious insects, there were 8 species of Laemophloeidae, an unidentified species of Staphylinidae, Microsicus spp. (Zopheridae), and Europs sp. (Monotomidae) (all Coleoptera). Among the parasitoids, hymenopterans of the families Braconidae, Eulophidae, Pteromalidae, Encyrtidae, Eupelmidae, and Bethylidae emerged from wood containing various species of Scolytinae. However, the only specimens that emerged from logs in which X. glabratus was present were Bethylidae, Braconidae, Encyrtidae (perhaps Closterocerus sp.), and Scelionidae. Four hymenopteran species were collected using attractants in Florida and Taiwan. However, more studies are needed to clarify their role as natural enemies of X. glabratus. C1 [Pena, Jorge E.; Carrillo, Daniel; Duncan, Rita E.] Univ Florida, Trop Res & Educ Ctr, Homestead, FL 33031 USA. [Weihman, Scott W.; Roda, Amy L.] USDA, APHIS PPQ CPHST, Miami, FL USA. [McLean, Stephen] Univ Florida, Entomol & Nematol, Gainesville, FL USA. [Cave, Ronald D.] Univ Florida, Indian River REC, Ft Pierce, FL USA. [Evans, Gregory] NIS, USDA, APHIS, Beltsville, MD USA. [Krauth, Stephen] Univ Wisconsin, Madison, WI USA. [Thomas, M. C.] Florida Dept Agr & Consumer Serv, Gainesville, FL USA. [Lu, S. S.] Natl Inst Forestry, Taipei, Taiwan. [Kendra, Paul E.] ARS, USDA, Miami, FL USA. RP Pena, JE (reprint author), Univ Florida, Trop Res & Educ Ctr, 18905 SW 280th St, Homestead, FL 33031 USA. EM jepena@ufl.edu FU USDA APHIS PPQ FX We thank D. Long (USDA ARS) for technical assistance, and H. Frank (University of Florida) and Ken Bloem (USDA APHIS) for critical reviews of the manuscript. This research was financially supported by 2 grants to JEP from USDA APHIS PPQ. NR 24 TC 0 Z9 0 U1 4 U2 12 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD SEP PY 2015 VL 98 IS 3 PG 8 WC Entomology SC Entomology GA CS2QC UT WOS:000361915000013 ER PT J AU Porter, SD Valles, SM Gavilanez-Slone, JM AF Porter, Sanford D. Valles, Steven M. Gavilanez-Slone, Jenny M. TI Long-term efficacy of two cricket and two liver diets for rearing laboratory fire ant colonies (Hymenoptera: Formicidae: Solenopsis invicta) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Acheta domesticus; Gryllodes sigillatus; banded cricket; brood production; raw liver; red imported fire ant ID VIRUS 3; TEMPERATURE; GROWTH; IMPACT; RATES AB Fire ant colonies thrived on diets of sugar water and either raw beef liver or raw chicken liver for almost 8 mo, after which brood production ceased in half of these colonies. As expected, colonies did well on a diet of sugar water and domestic crickets. Unexpectedly, however, banded crickets were much less suitable than either liver or domestic crickets. C1 [Porter, Sanford D.; Valles, Steven M.; Gavilanez-Slone, Jenny M.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. RP Porter, SD (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM sanford.porter@ars.usda.gov NR 13 TC 0 Z9 0 U1 2 U2 6 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD SEP PY 2015 VL 98 IS 3 PG 3 WC Entomology SC Entomology GA CS2QC UT WOS:000361915000033 ER PT J AU Tillman, PG AF Tillman, P. Glynn TI First record of Sesbania punicea (Fabales: Fabaceae) as a host plant for Chinavia hilaris (Hemiptera: Pentatomidae) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE rattlebox; green stink bug; feeding; egg parasitoid ID STINK BUGS HETEROPTERA; POPULATION-DYNAMICS; COTTON; DAMAGE AB Sesbania punicea (Cav.) Benth. (Fabales: Fabaceae) plants in a field border surrounding a cotton field were sampled for Chinavia hilaris (Say) (Hemiptera: Pentatomidae) to determine if this leguminous shrub served as a host for this stink bug. Chinavia hilaris adults and nymphs fed on fruit of this plant, and nymphs developed on the plant. This is the first record of S. punicea as a host for this stink bug. C1 ARS, USDA, Crop Protect & Management Res Lab, Tifton, GA 31793 USA. RP Tillman, PG (reprint author), ARS, USDA, Crop Protect & Management Res Lab, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 11 TC 0 Z9 0 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD SEP PY 2015 VL 98 IS 3 PG 2 WC Entomology SC Entomology GA CS2QC UT WOS:000361915000032 ER PT J AU Werle, CT Chong, JH Sampson, BJ Reding, ME Adamczyk, JJ AF Werle, Christopher T. Chong, Juang-Horng Sampson, Blair J. Reding, Michael E. Adamczyk, John J. TI Seasonal and spatial dispersal patterns of select ambrosia beetles (Coleoptera: Curculionidae) from forest habitats into production nurseries SO FLORIDA ENTOMOLOGIST LA English DT Article DE push-pull; volatile; IPM; population ecology; Xyleborina; trap ID ETHANOL-BAITED TRAPS; XYLOSANDRUS-GERMANUS; ORNAMENTAL NURSERIES; PEST-MANAGEMENT; LONGLEAF PINE; SCOLYTIDAE; ERADICATION; TENNESSEE; CAPTURES; INSECTS AB Exotic ambrosia beetles (Coleoptera: Curculionidae) are important pests of ornamental tree nurseries. Although these beetles reportedly disperse in early spring from peripheral forested areas into nurseries, few studies have determined how far they fly to infest new host trees, or whether a mass-trapping strategy can adequately protect a nursery crop. Field monitoring with ethanol baits in South Carolina (2011-2012), Mississippi (2013-2014), and Louisiana (2013-2014), USA, determined the timing of peak ambrosia beetle flights, dispersal distance, and optimal trap location. In addition to the well-documented spring flight peak, southeastern nursery managers may need to be aware of a second, late-summer flight. Captures from traps placed in a nursery at various distances (-25 to 200 m) from the forest-nursery interface showed a significant linear and quadratic trend in decreasing numbers of beetles captured with increasing distance from the forest in South Carolina, whereas significant linear, quadratic, and cubic trends were detected in Louisiana and Mississippi. Although captures at the nursery edge were lower than within the forest, traps placed at the nursery edge may still represent the optimal tool for both monitoring and mass-trapping programs because of easier access for personnel. Susceptible tree cultivars may gain added protection when placed deeper within nursery interiors and when baited traps line adjacent nursery edges. C1 [Werle, Christopher T.; Sampson, Blair J.; Adamczyk, John J.] USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. [Chong, Juang-Horng] Clemson Univ, Pee Dee Res & Educ Ctr, Florence, SC 29506 USA. [Reding, Michael E.] USDA ARS, Hort Insects Res Lab, Wooster, OH 44691 USA. RP Werle, CT (reprint author), USDA ARS, Thad Cochran Southern Hort Lab, 810 Hwy 26 W, Poplarville, MS 39470 USA. EM chris.werle@ars.usda.gov FU Greenforest Nursery; Bracy's Nursery; Parsons Nursery; King's Sunset Nursery; United States Department of Agriculture (USDA) Agricultural Research Service (ARS) Floriculture and Nursery Research Initiative, ARS Research Project [6062-21430-002-00D]; USDA National Institute of Food and Agriculture [SC-1700473] FX We thank Chris Ranger (ARS-HIRL, Wooster, Ohio), Peter Schultz (Hampton Roads AREC, Virginia Beach, Virginia), Jason Oliver and Karla Addesso (Tennessee State University, OFNRC, McMinnville, Tennessee), and Jeff Kuehny, Tim Schowalter, and Jeff Beasley (Louisiana State University, Baton Rouge, Louisiana) for advice on research methods. Also, thanks to Greenforest Nursery, Bracy's Nursery, Parsons Nursery, and King's Sunset Nursery for continuing cooperation and support of our research. This work was supported in part by the United States Department of Agriculture (USDA) Agricultural Research Service (ARS) Floriculture and Nursery Research Initiative, ARS Research Project 6062-21430-002-00D (National Program 305-Crop Production), and the USDA National Institute of Food and Agriculture under project number SC-1700473. NR 31 TC 2 Z9 2 U1 2 U2 70 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD SEP PY 2015 VL 98 IS 3 PG 8 WC Entomology SC Entomology GA CS2QC UT WOS:000361915000010 ER PT J AU Jones, RF Bosland, PW Steiner, RL Jones, RW O'Connel, MA AF Jones, Rhiana F. Bosland, Paul W. Steiner, Robert L. Jones, Richard W. O'Connel, Mary A. TI Detection of Gene Expression Changes in Capsicum annuum L. Foliar Blight Caused by Phytophthora capsici Leon. Using qRT-PCR and Leaf Discs SO HORTSCIENCE LA English DT Article DE chile peppers; disease resistance; disease syndrome; races ID RACE-SPECIFIC RESISTANCE; ROOT-ROT; INHIBITOR PROTEIN; CHILE PEPPER; CELL-WALL; FRUIT; QUANTIFICATION; ACCUMULATION; INHERITANCE; STEM AB Phytophthora capsici is responsible for multiple disease syndromes of Capsicum annuum but the resistance mechanism is still unknown. Evaluating gene expression during foliar blight can be used to identify expression patterns associated with resistance in Capsicum species. This study reports a direct comparison of gene expression changes during the foliar blight syndrome using two different races of P. capsici on C. annuum host plants with resistant and susceptible phenotypes to those races. Four genes were evaluated for differential expression following leaf inoculation with P. capsici. RNA isolated from leaves at three time points was used to quantify gene expression by quantitative real-time polymerase chain reaction (qRT-PCR). Of four genes tested, two had differential expression in response to P. capsici at 72 hours postinoculation, a xyloglucan-specific endo-beta-1,4-glucanase inhibitor protein (XEGIP2) in susceptible cultivar New Mexico Heritage 6-4 (NMH6-4), and a C. annuum cell wall protein (CWP) in resistant Criollo de Morelos 334 (CM334). Both genes had a 5-fold increase in transcription in leaves over the control. These results suggest that both genes are playing a role in disease resistance to foliar blight. C1 [Jones, Rhiana F.; Bosland, Paul W.; O'Connel, Mary A.] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Steiner, Robert L.] New Mexico State Univ, Dept Econ & Int Business, Las Cruces, NM 88003 USA. [Jones, Richard W.] USDA ARS, Beltsville Agr Res Ctr, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. RP O'Connel, MA (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM moconnel@nmsu.edu FU New Mexico Agricultural Station; U.S. Department of Agriculture Agricultural Research Service through a Specific Cooperative Agreement FX This work was supported in part by the New Mexico Agricultural Station, and support from the U.S. Department of Agriculture Agricultural Research Service through a Specific Cooperative Agreement. NR 37 TC 0 Z9 0 U1 3 U2 9 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD SEP PY 2015 VL 50 IS 9 BP 1342 EP 1348 PG 7 WC Horticulture SC Agriculture GA CS8BD UT WOS:000362309800014 ER PT J AU Sun, XX Baldwin, E Ritenour, M Plotto, A Bai, JH AF Sun, Xiuxiu Baldwin, Elizabeth Ritenour, Mark Plotto, Anne Bai, Jinhe TI Evaluation of Natural Colorants and Their Application on Citrus Fruit as Alternatives to Citrus Red No. 2 SO HORTSCIENCE LA English DT Article DE annatto; paprika; degreening; waxing ID BIXA-ORELLANA L.; PERFORMANCE LIQUID-CHROMATOGRAPHY; FUNGAL PIGMENTS; FOOD COLORANTS; MODEL SYSTEM; ANNATTO; STABILITY; LIGHT; CAROTENOIDS; PAPRIKA AB Warm field temperatures can often result in poor peel color of some citrus varieties, especially early in the harvest season. Under these conditions, Florida oranges, temples, tangelos, and K-Early citrus fruit are allowed to be treated with Citrus Red No.2 dye (CR2) to help produce a more acceptable peel color. Unfortunately, CR2, the commercial colorant used in Florida, has been listed as a group 2B carcinogen by the European Union (EU) and the International Agency for Research on Cancer (IARC). Although not likely dangerous at levels used on citrus, and on a part of the fruit that is not ingested, there is a negative health perception, and thus, a need for natural or food grade alternative colorants to replace CR2 for use on citrus. This research demonstrated that three out of five oil-soluble natural red/orange colorants resulted in peel colors somewhat similar to the industry standard CR2. These three (annatto extract, paprika extract, and paprika oleoresin) were selected for further in vivo studies. The stability of the natural colorants along with CR2 was evaluated by applying them on test papers and then on fresh 'Hamlin' oranges. All natural colorants were found to be easily oxidized and faded when applied on test papers. However, coating the colored surfaces with carnauba wax apparently inhibited oxidation and the subsequent discoloration of the surface. When applying the natural colorants to 'Hamlin' oranges before waxing, the treatments retained the improved color after storage in the dark at 5 degrees C, simulating cold storage. However, only annatto extract maintained a stable color when subsequently stored in a simulated market condition, at 23 degrees C exposed to 300 lx of standard fluorescent white light. C1 [Sun, Xiuxiu; Baldwin, Elizabeth; Plotto, Anne; Bai, Jinhe] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Ritenour, Mark] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL 34845 USA. RP Bai, JH (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM Jinhe.bai@ars.usda.gov NR 44 TC 0 Z9 0 U1 2 U2 14 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD SEP PY 2015 VL 50 IS 9 BP 1353 EP 1357 PG 5 WC Horticulture SC Agriculture GA CS8BD UT WOS:000362309800016 ER PT J AU Wang, LB Baldwin, EA Yu, ZF Bai, JH AF Wang, Libin Baldwin, Elizabeth A. Yu, Zhifang Bai, Jinhe TI The Impact of Kitchen and Food Service Preparation Practices on the Volatile Aroma Profile in Ripe Tomatoes: Effects of Refrigeration and Blanching SO HORTSCIENCE LA English DT Article DE Solanum lycopersicum; aromatic volatile; blanching; refrigeration; electronic nose ID CAPSICUM-ANNUUM L.; CHILLING TEMPERATURE; FLAVOR VOLATILES; HEAT-TREATMENTS; STORAGE-TEMPERATURE; HYDROPEROXIDE LYASE; FRUIT; QUALITY; RESISTANCE; INJURY AB Both refrigeration and blanching of red-stage tomatoes are common practices in Japan home kitchens and in food service operations. However, little is reported on the impact of such practices on aroma profiles in tomato fruits. In this study, 'FL 47' tomatoes at red stage were dipped in 50 degrees C hot water for 5 minutes or exposed to 5 degrees C for 4 days to simulate consumer handling of tomato in food service or home kitchens, respectively. Of the 42 volatile compounds detected, refrigeration generally suppressed production of aldehydes, alcohols, oxygen-containing heterocyclic compounds, and nitrogen- and oxygen-containing heterocyclic compounds, including the following abundant and/or important volatiles: pentanal, 3-methylbutanal, 2-methylbutanal, hexanal, cis-3-hexenal, trans-2-hexenal, 2-phenylacetaldehyde, pentanol, 3-methylbutanol, 2-phenylethanol, 1-penten-3-one, geranial, and geranylacetone. On the other hand, the production of aldehydes, alcohols, hydrocarbons, oxygen-containing heterocyclic compounds, and nitrogen- and oxygen-containing heterocyclic compounds was reduced by blanching, associated with low concentrations of 2-methylbutanal, pentanal, cis-3-hexenal, trans-2-hexenal, 2-phenylacetaldehyde, pentanol, 2-methylbutanol, and 2-phenylethanol. These results indicate that a short blanching or refrigeration of tomatoes substantially impacts tomato aroma quality. C1 [Wang, Libin; Baldwin, Elizabeth A.; Bai, Jinhe] USDA, US Hort Res Lab, Agr Res Serv, Ft Pierce, FL 34945 USA. [Wang, Libin; Yu, Zhifang] Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. RP Bai, JH (reprint author), USDA, US Hort Res Lab, Agr Res Serv, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov FU China Scholarship Council [201306850049]; Postgraduate Program in Jiangsu Province [CXLX13_267]; Big Red Tomato Packers, Fort Pierce, FL FX We thank the financial support to the experiment from China Scholarship Council (201306850049), Postgraduate Program in Jiangsu Province (CXLX13_267), and the Big Red Tomato Packers, Fort Pierce, FL. Mention of a trademark or proprietary product is for identification only and does not imply a guarantee or warranty of the product by the U.S. Department of Agriculture. The U.S. Department of Agriculture prohibits discrimination in all its programs and activities on the basis of race, color, national origin, gender, religion, age, disability, political beliefs, sexual orientation, and marital or family status NR 49 TC 2 Z9 2 U1 5 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD SEP PY 2015 VL 50 IS 9 BP 1358 EP 1364 PG 7 WC Horticulture SC Agriculture GA CS8BD UT WOS:000362309800017 ER PT J AU Wei, J Qiu, XY Chen, L Hu, WJ Hu, RB Chen, J Sun, L Li, L Zhang, H Lv, ZQ Shen, GX AF Wei, Jia Qiu, Xiaoyun Chen, Lin Hu, Wenjun Hu, Rongbin Chen, Jian Sun, Li Li, Li Zhang, Hong Lv, Zhiqiang Shen, Guoxin TI The E3 ligase AtCHIP positively regulates Clp proteolytic subunit homeostasis SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE AtCHIP; chloroplast protease; Clp proteolytic subunits; ClpP4; ClpP3; ClpP5; protein homeostasis ID REPEAT-CONTAINING PROTEIN; ARABIDOPSIS-THALIANA; MUTANT REVEALS; FUNCTIONAL IMPLICATIONS; CHLOROPLAST DEVELOPMENT; PLANT DEVELOPMENT; UBIQUITIN LIGASE; COMPLEX; STRESS; GENE AB AtCHIP positively regulates Clp subunit degradation by selective ubiquitylation of their precursors in cytosol, providing new evidence for the E3 ligase-based proteasome system regulating nuclear-encoded organelle protein biogenesis and homeostasis.The caseinolytic peptidase (Clp) core proteins are essential for plant growth and development, especially for chloroplast function. Antisense or overexpression of ClpP4, which is one of the Clp core subunits, causes chlorotic phenotypes in Arabidopsis. An E3 ligase gene, AtCHIP, has previously been found to ubiquitylate ClpP4 in vitro. ClpP4 antisense and overexpressing plants that also overexpressed AtCHIP were constructed to explore the effect of AtCHIP on ClpP4. Overexpression of AtCHIP was found to rescue the chlorotic phenotypes of both ClpP4 antisense and overexpressing plants. The unbalanced levels of Clp core proteins in ClpP4 antisense and overexpressing plants with overexpression of AtCHIP were similar to wild-type levels, suggesting that AtCHIP regulates Clp core proteins. The results also show that AtCHIP can interact with ClpP3 and ClpP5 in yeast and ubiquitylate ClpP3 and ClpP5 in vitro. This suggests that AtCHIP is directly related to ClpP3 and ClpP5. Given these results, the inference is that through selective degradation of Clp subunits, AtCHIP could positively regulate homeostasis of Clp proteolytic subunits and maximize the production of functional chloroplasts. Similar results were obtained from transgenic tobacco plants, suggesting that regulation of the Clp protease by AtCHIP is conserved. C1 [Wei, Jia; Qiu, Xiaoyun; Chen, Lin; Hu, Wenjun; Lv, Zhiqiang; Shen, Guoxin] Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China. [Hu, Rongbin; Chen, Jian; Sun, Li; Zhang, Hong] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. [Li, Li] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Li, Li] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Shen, GX (reprint author), Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China. EM guoxin.shen@ttu.edu RI HU, RONGBIN/B-2225-2017; OI HU, RONGBIN/0000-0001-5921-6891 FU National Natural Science Foundation of China [31170793, 31300259, 31402140]; Zhejiang Province [LY15C020002]; State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control [2010DS700124-KF1405]; International Cooperation, Innovation and Upgrading programs of Zhejiang Academy of Agricultural Sciences [2014CX005]; Shaoxing 330 Overseas Elites Program FX This project was supported by grants from the National Natural Science Foundation of China (31170793, 31300259, 31402140) and Zhejiang Province LY15C020002; the State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control (2010DS700124-KF1405); the International Cooperation, Innovation and Upgrading programs of Zhejiang Academy of Agricultural Sciences (2014CX005); and the Shaoxing 330 Overseas Elites Program. NR 43 TC 1 Z9 3 U1 2 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD SEP PY 2015 VL 66 IS 19 BP 5809 EP 5820 DI 10.1093/jxb/erv286 PG 12 WC Plant Sciences SC Plant Sciences GA CS4VF UT WOS:000362073400014 PM 26085677 ER PT J AU Barrios-Masias, FH Knipfer, T McElrone, AJ AF Barrios-Masias, F. H. Knipfer, T. McElrone, A. J. TI Differential responses of grapevine rootstocks to water stress are associated with adjustments in fine root hydraulic physiology and suberization SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Drought resistance; fine root conductivity; Lp; root hydraulics; traits; Vitis ID CROP IMPROVEMENT; XYLEM VESSELS; WHOLE-PLANT; MAIZE ROOTS; SCION VIGOR; TRANSPORT; DROUGHT; PRESSURE; CONDUCTANCE; SOIL AB Drought resistance in a grapevine rootstock was associated with root traits that maintain higher water uptake capacity via less suberization so roots can resupply the shoot with water upon rewatering.Water deficits are known to alter fine root structure and function, but little is known about how these responses contribute to differences in drought resistance across grapevine rootstocks. The ways in which water deficit affects root anatomical and physiological characteristics were studied in two grapevine rootstocks considered as low-medium (101-14Mgt) and highly (110R) drought resistant. Rootstocks were grown under prolonged and repeated drying cycles or frequent watering ('dry' and 'wet' treatments, respectively), and the following parameters were evaluated: root osmotic and hydrostatic hydraulic conductivity (Lp (os) and Lp (hyd), respectively), suberization, steady-state root pressure (P (rs)), sap exudation rates, sap osmotic potential, and exosmotic relaxation curves. For both rootstocks, the 'dry' treatment reduced fine root Lp, elicited earlier root suberization and higher sap osmotic potential, and generated greater P (rs) after rewatering, but the rootstocks responded differently under these conditions. Lp (os), Lp (hyd), and sap exudation rates were significantly higher in 110R than in 101-14Mgt, regardless of moisture treatment. Under 'dry' conditions, 110R maintained a similar Lp (os) and decreased the Lp (hyd) by 36% compared with 'wet' conditions, while both parameters were decreased by at least 50% for 101-14Mgt under 'dry' conditions. Interestingly, build-up of P (rs) in 110R was 34% lower on average than in 101-14Mgt, suggesting differences in the development of suberized apoplastic barriers between the rootstocks as visualized by analysis of suberization from fluorescence microscopy. Consistent with this pattern, 110R exhibited the greatest exosmotic Lp (os) (i.e. Lp (os) of water flowing from roots to the soil) as determined from relaxation curves under wet conditions, where backflow may have limited its capacity to generate positive xylem pressure. The traits studied here can be used in combination to provide new insights needed for screening drought resistance across grapevine rootstocks. C1 [Barrios-Masias, F. H.; Knipfer, T.; McElrone, A. J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [McElrone, A. J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP McElrone, AJ (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM ajmcelrone@ucdavis.edu FU National Institute of Food and Agriculture-Specialty Crops Research Initiative grant; American Vineyard Foundation; US Department of Agriculture-Agricultural Research Service Current Research Information System [5306-21220-004-00] FX This work was supported by a National Institute of Food and Agriculture-Specialty Crops Research Initiative grant and funding from the American Vineyard Foundation to AJM, and by the US Department of Agriculture-Agricultural Research Service Current Research Information System (research project no. 5306-21220-004-00). NR 49 TC 7 Z9 7 U1 5 U2 39 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD SEP PY 2015 VL 66 IS 19 BP 6069 EP 6078 DI 10.1093/jxb/erv324 PG 10 WC Plant Sciences SC Plant Sciences GA CS4VF UT WOS:000362073400033 PM 26160580 ER PT J AU Tarara, JM Pena, JEP AF Tarara, Julie M. Pena, Jorge E. Perez TI Moderate Water Stress from Regulated Deficit Irrigation Decreases Transpiration Similarly to Net Carbon Exchange in Grapevine Canopies SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE water use efficiency; canopy conductance; photosynthesis; whole-canopy gas exchange; Vitis vinifera ID VITIS-VINIFERA L.; FIELD-GROWN GRAPEVINES; USE EFFICIENCY; GAS-EXCHANGE; WHOLE-CANOPY; STOMATAL CONDUCTANCE; DROUGHT; PHOTOSYNTHESIS; RESPONSES; SOIL AB To determine the effects of timing and extent of regulated deficit irrigation (RDI) on grapevine (Vitis vinifera) canopies, whole-canopy transpiration (Tr-V) and canopy conductance to water vapor (g(c)) were calculated from whole-vine gas exchange near key stages of fruit development. The vines were managed under three approaches to RDI: 1) standard industry practice (RDIS), or weekly replacement of 60% to 70% of estimated evapotranspiration (ET) for well-watered grapevines; 2) early additional deficit (RDIE), or one-half of RDIs applied between fruit set and veraison; and 3) late additional deficit (RDIL), or one-half of RDIS applied between veraison and harvest. Compared with RDIS, the additional deficits (RDIE, RDIL) reduced daily cumulative Tr-V by about 45% (RDIE) and about 48% [RDIL (57% by unit leaf area)]. Diurnal patterns of g(c) indicated consistent moderate water stress in all RDI regimens (g(c) approximate to 50-150 mmol m(-2)s(-1)). Under RDIE and RDIL, there were transient occurrences of severe water stress, indicated by g(c) declining below 50 mmol m(-2)s(-1). Across the day, vines under RDIE and RDIL had lower g(c) than RDIS. Under all deficit regimens, Tr-V exhibited opposing hysteretic loops with solar radiation [photosynthetic photon flux (PPF)] and vapor pressure deficit (VPD), with less sensitivity to VPD in RDIE and RDIL. For a given value of VPD, Tr-V was higher in the morning than in the afternoon. For a given value of PPF, Tr-V was higher in the afternoon than in the morning. Single-leaf measurements of transpiration overestimated Tr-V by an average of 45%. Instantaneous water use efficiency (WUE) declined during midday at the pre- and postveraison measurements for all RDI regimens. Wholecanopy daily integrated WUE (WUEd) did not differ among regimens during the additional deficits because daily cumulative values of whole-vine net carbon exchange (NCEV) and Tr-V changed proportionally: by about 43% to 46% in RDIE relative to RBIS. The case was less clear-cut for RDIL, where NCEV declined by 33% and Tr-V by 48% relative to RDIS. However, WUEd did not differ significantly between the two. More substantial water deficits than those are currently practiced in the industry through RDI could be used for potential water savings in semiarid climates. C1 [Tarara, Julie M.] USDA ARS, Hort Crops Res Unit, Prosser, WA 99350 USA. [Pena, Jorge E. Perez] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Tarara, JM (reprint author), Ste Michelle Wine Estates,660 Frontier Rd, Prosser, WA 99350 USA. EM julie.tarara@smwe.com FU USDA-ARS CRIS [5358-21000-025-D]; USDA-ARS Northwest Center for Small Fruits Research; USDA-CSREES-Viticulture Consortium; WSU Department of Horticulture and Landscape Architecture; American Vineyard Foundation; Washington Wine Advisory Committee FX Funding for this project was provided by USDA-ARS CRIS no.5358-21000-025-D, USDA-ARS Northwest Center for Small Fruits Research, USDA-CSREES-Viticulture Consortium, WSU Department of Horticulture and Landscape Architecture, American Vineyard Foundation, and Washington Wine Advisory Committee. NR 57 TC 4 Z9 4 U1 1 U2 15 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD SEP PY 2015 VL 140 IS 5 BP 413 EP 426 PG 14 WC Horticulture SC Agriculture GA CS4QM UT WOS:000362061000004 ER PT J AU Othman, Y Steele, C VanLeeuwen, D St Hilaire, R AF Othman, Yahia Steele, Caiti VanLeeuwen, Dawn St Hilaire, Rolston TI Hyperspectral Surface Reflectance Data Detect Low Moisture Status of Pecan Orchards during Flood Irrigation SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE vegetation index; remote sensing; Carya illinoinensis; water stress; soil reflectance ID VEGETATION WATER-CONTENT; LEAF-AREA INDEX; SPECTRAL REFLECTANCE; ABSORPTION FEATURES; STRESS DETECTION; USE EFFICIENCY; LIQUID WATER; CANOPY; PHOTOSYNTHESIS; TREES AB For large fields, remote sensing might permit plant low moisture status to be detected early, and this may improve drought detection and monitoring. The objective of this study was to determine whether canopy and soil surface reflectance data derived from a handheld spectroradiometer can detect moisture status assessed using midday stem water potential (psi(smd)) in pecan (Carya illinoinensis) during cyclic flood irrigations. We conducted the study simultaneously on two mature pecan orchards, one in a sandy loam (La Mancha) and the other in a clay loam (Leyendecker) soil. We were particularly interested in detecting moisture status in the -0.90 to -1.5 MPa psi(smd) range because our previous studies indicated this was the critical range for irrigating pecan. Midday stem water potential, photosynthesis (A) and canopy and soil surface reflectance measurements were taken over the course of irrigation dry-down cycles at psi(smd) levels of -0.40 to -0.85 MPa (well watered) and -0.9 to -1.5 MPa (water deficit). The decline in A averaged 34% in La Mancha and 25% in Leyendecker orchard when psi(smd) ranged from -0.9 to -1.5 MPa. Average canopy surface reflectance of well-watered trees (psi(smd) -0.4 to -0.85 MPa) was significantly higher than the same trees experiencing water deficits (psi(smd) -0.9 to -1.5 MPa) within the 350- to 2500-nm bands range. Conversely, soil surface reflectance of well-watered trees was lower than water deficit trees over all bands. At both orchards, coefficient of determinations between psi(smd) and all soil and canopy bands and surface reflectance indices were less than 0.62. But discriminant analysis models derived from combining soil and canopy reflectance data of well-watered and water-deficit trees had high classification accuracy (overall and cross-validation classification accuracy >80%). A discriminant model that included triangular vegetation index (TVI), photochemical reflectance index (PRI), and normalized soil moisture index (NSMI) had 85% overall accuracy and 82% cross-validation accuracy at La Mancha orchard. At Leyendecker, either a discriminant model weighted with two soil bands (690 and 2430 nm) or a discriminant model that used PRI and soil band 2430 nm had an overall classification and cross-validation accuracy of 99%. In summary, the results presented here suggest that canopy and soil hyperspectral data derived from a handheld spectroradiometer hold promise for discerning the psi(smd) of pecan orchards subjected to flood irrigation. C1 [Othman, Yahia; St Hilaire, Rolston] New Mexico State Univ, Plant & Environm Sci, Las Cruces, NM 88003 USA. [Steele, Caiti] New Mexico State Univ, Jornada Expt Range, USDA ARS, Las Cruces, NM 88003 USA. [VanLeeuwen, Dawn] New Mexico State Univ, Econ Appl Stat & Int Business, Las Cruces, NM 88003 USA. RP St Hilaire, R (reprint author), New Mexico State Univ, Plant & Environm Sci, Las Cruces, NM 88003 USA. EM rsthilai@nmsu.edu FU New Mexico State University Agricultural Experiment Station FX This work is supported by the New Mexico State University Agricultural Experiment Station. We are very grateful to Cameron Radosevich for his help with collecting field data. We also thank Richard Heerema and Salim Bawazir for their critical review of this manuscript. We acknowledge Mr. Midray Clark for allowing us to Use his pecan orchard. NR 46 TC 0 Z9 0 U1 2 U2 9 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD SEP PY 2015 VL 140 IS 5 BP 449 EP 458 PG 10 WC Horticulture SC Agriculture GA CS4QM UT WOS:000362061000007 ER PT J AU Baldwin, EA Scott, JW Bai, JH AF Baldwin, Elizabeth A. Scott, John W. Bai, Jinhe TI Sensory and Chemical Flavor Analyses of Tomato Genotypes Grown in Florida during Three Different Growing Seasons in Multiple Years SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE Solanum lycopersicum; breeding; sugars; acids; aroma; volatiles; sweet; sour ID LYCOPERSICON-ESCULENTUM MILL; QUALITY; FRUIT; VOLATILE; AROMA; DESCRIPTORS; PERCEPTION; TEMPERATURE; SUGARS AB Thirty-eight tomato (Solanum lycopersicum L.) genotypes were analyzed for sensory attributes "sweet," "sour," and "overall flavor" over 7 years, one to three seasons per year (March, June, and December) as well as for physical and chemical flavor-related attributes including color, sugars, acids, and aroma volatiles (6-7 years). Principal component analysis, of the data of nine genotypes showed that for harvest season, December-harvested fruit were generally associated with more acids and sourness perception and less sugars and sweetness perception and, therefore, lower overall flavor ratings compared with June-harvested fruit. March-harvested samples were intermediate. Despite the seasonal variations, there were significant differences between genotypes for sensory perception of sweetness, sourness, and flavor, between seasons for sourness and flavor, and between years for flavor, with some interactions between genotypes, seasons, and years. In addition to sugar and acid measurements, 29 aroma volatiles were evaluated in 33 genotypes over the seasons. Eleven volatiles were found to positively correlate with flavor perception and 13 enhanced flavor along with the soluble solids/titratable acidity ratio in a two-predictor model, providing aroma targets for breeders. Among the genotypes evaluated most frequently were the Florida industry standard 'Florida 47' and University of Florida hybrid 'Fla. 8153' which was released in 2006 and is now marketed as Tasti-Lee (R). 'Florida 47' was almost always rated lower for sweet and overall flavor compared with 'Fla. 8153'. On a 1-9 hedonic scale, where 1 was least sweet, sour, or flavorful and 9 was most sweet, sour, or flavorful, average scores over the 7 years were 3.8 and 5.1 for sweet and 4.1 and 5.7 for overall flavor for 'Florida 47' and 'Fla. 8153', respectively. Other genotypes related to 'Fla. 8153', including its parents, were also rated high for sweet and overall flavor compared with 'Florida 47' and other commercial cultivars grown in Florida. Correspondingly, sugar measurements were higher, while acid measurements were slightly lower for 'Fla. 8153' compared with 'Florida 47'. Thirteen out of 29 aroma compounds showed differences between these two genotypes, with eight being higher in 'Fla. 8153' (including many fruity/floral notes) and four higher in Florida 47 (C-5 and C-6 aldehydes and alcohols giving green notes). This provides a useful chemical model for two genotypes that differ in flavor quality that can be exploited by breeders seeking to improve flavor. C1 [Baldwin, Elizabeth A.; Bai, Jinhe] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. [Scott, John W.] Univ Florida, Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. RP Baldwin, EA (reprint author), USDA ARS, Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM Liz.Baldwin@ars.usda.gov FU Florida Tomato Committee FX This work was partially funded by the Florida Tomato Committee. NR 42 TC 2 Z9 2 U1 6 U2 23 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD SEP PY 2015 VL 140 IS 5 BP 490 EP 503 PG 14 WC Horticulture SC Agriculture GA CS4QM UT WOS:000362061000012 ER PT J AU Meldrum, JR Champ, PA Brenkert-Smith, H Warziniack, T Barth, CM Falk, LC AF Meldrum, James R. Champ, Patricia A. Brenkert-Smith, Hannah Warziniack, Travis Barth, Christopher M. Falk, Lilia C. TI Understanding Gaps Between the Risk Perceptions of Wildland-Urban Interface (WUI) Residents and Wildfire Professionals SO RISK ANALYSIS LA English DT Article DE Risk assessment; risk gap; risk perception; wildfire; wildland-urban interface ID MITIGATION BEHAVIOR; FOREST LANDOWNERS; CHANGING CLIMATE; LAY JUDGMENTS; UNITED-STATES; FIRE RISK; HAZARD; MANAGEMENT; COMMUNICATION; STRATEGIES AB Research across a variety of risk domains finds that the risk perceptions of professionals and the public differ. Such risk perception gaps occur if professionals and the public understand individual risk factors differently or if they aggregate risk factors into overall risk differently. The nature of such divergences, whether based on objective inaccuracies or on differing perspectives, is important to understand. However, evidence of risk perception gaps typically pertains to general, overall risk levels; evidence of and details about mismatches between the specific level of risk faced by individuals and their perceptions of that risk is less available. We examine these issues with a paired data set of professional and resident assessments of parcel-level wildfire risk for private property in a wildland-urban interface community located in western Colorado, United States. We find evidence of a gap between the parcel-level risk assessments of a wildfire professional and numerous measures of residents' risk assessments. Overall risk ratings diverge for the majority of properties, as do judgments about many specific property attributes and about the relative contribution of these attributes to a property's overall level of risk. However, overall risk gaps are not well explained by many factors commonly found to relate to risk perceptions. Understanding the nature of these risk perception gaps can facilitate improved communication by wildfire professionals about how risks can be mitigated on private lands. These results also speak to the general nature of individual-level risk perception. C1 [Meldrum, James R.; Brenkert-Smith, Hannah] Univ Colorado, Inst Behav Sci, Boulder, CO 80309 USA. [Champ, Patricia A.; Warziniack, Travis] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Barth, Christopher M.] Bur Land Management, Southwest Dist Fire Management Program, Montrose, CO 81401 USA. [Falk, Lilia C.] West Reg Wildfire Council, Montrose, CO 81401 USA. RP Meldrum, JR (reprint author), Univ Colorado, Inst Behav Sci, Boulder, CO 80309 USA. EM james.meldrum@colorado.edu OI Meldrum, James/0000-0001-5250-3759 FU U.S. Interagency National Fire Plan, Forest Service [13-CS-11221636-036] FX We thank the West Region Wildfire Council (WRWC) for providing survey and assessment data. The research reported in this article was carried out in the Research Program on Environment and Society in the Institute of Behavioral Science, University of Colorado Boulder. This work was improved by the constructive comments of two anonymous reviewers and participants of numerous professional meetings. Funding provided by the U.S. Interagency National Fire Plan, Forest Service agreement number 13-CS-11221636-036. NR 73 TC 0 Z9 0 U1 1 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD SEP PY 2015 VL 35 IS 9 BP 1746 EP 1761 DI 10.1111/risa.12370 PG 16 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA CS6YB UT WOS:000362228600012 PM 26036435 ER PT J AU Pillon, Y Lucas, E Johansen, JB Sakishima, T Hall, B Geib, SM Stacy, EA AF Pillon, Yohan Lucas, Eve Johansen, Jennifer B. Sakishima, Tomoko Hall, Brian Geib, Scott M. Stacy, Elizabeth A. TI An Expanded Metrosideros (Myrtaceae) to Include Carpolepis and Tepualia Based on Nuclear Genes SO SYSTEMATIC BOTANY LA English DT Article DE Carpolepis; Metrosideros; Myrtaceae; Pacific; phylogeny; Tepualia ID MOLECULAR SYSTEMATICS; PHYLOGENETIC ANALYSES; EVOLUTION; PACIFIC; TREE; PHYLOGENOMICS; BACKHOUSIEAE; CONCORDANCE; POLYMORPHA; DISPERSAL AB The genus Metrosideros (Myrtaceae) comprises 50-60 species found largely across the Pacific Islands. The relationships within this genus, including the circumscriptions of the subgenera Mearnsia and Metrosideros and their relationships with the other members of the tribe Metrosidereae (namely the New Caledonian endemic genus Carpolepis and the South American Tepualia), are poorly understood. Phylogenetic analyses were carried out using previously published ITS sequences, covering most species of the tribe, and new sequences of five single-copy nuclear genes with a reduced taxon sampling. The independent and combined analyses of the five nuclear genes using a range of approaches, including Bayesian single-gene, concatenated (MrBayes), concordance (BUCKy) and coalescent (*BEAST) analyses, yielded different topologies, indicating important conflicts among phylogenies based on individual genes. The deep relationships within the tribe Metrosidereae remain poorly resolved, but our results indicate that the species of Carpolepis and Tepualia are likely nested in the genus Metrosideros. A broad circumscription of the genus Metrosideros is therefore adopted, and the new combinations Metrosideros laurifolia var. demonstrans, Metrosideros tardiflora and Metrosideros vitiensis are here published. C1 [Pillon, Yohan; Johansen, Jennifer B.; Sakishima, Tomoko; Stacy, Elizabeth A.] Univ Hawaii, Trop Conservat Biol & Environm Sci, Hilo, HI 96720 USA. [Lucas, Eve] Royal Bot Gardens, Herbarium, Richmond TW9 3AB, Surrey, England. [Hall, Brian; Geib, Scott M.] USDA ARS, Trop Crop & Commod Protect Res Unit, Daniel K Inouye Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Hall, Brian] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA. RP Pillon, Y (reprint author), Univ Hawaii, Trop Conservat Biol & Environm Sci, 200 West Kawili St, Hilo, HI 96720 USA. EM yohan.pillon@gmail.com FU NSF [0833211, 0954274] FX We thank Laure Barrabe, Martin Gardner (Royal Botanic Gardens, Edinburgh), Edith Kapinos (Kew DNA bank), Jean-Yves Meyer (Delegation a la recherche de la Polynesie Francaise), herbarium NOU, and the staff of the National Park of American Samoa for help with collection or for sending plant material. We thank Anne Veillet (Genetic Core lab at UH Hilo), Dan New, and Matt Settles (IBEST, University of Idaho) for help with sequencing procedures, Laure Barrabe, Neil Snow, Kanchi Gandhi, James Smith and an anonymous reviewer for comments on an earlier version of this manuscript. Funding was provided by NSF grants 0833211 and 0954274. Bioinformatic analysis was performed on computing resources at USDA-ARS Pacific Basin Agricultural Research Center (Moana cluster; Hilo, Hawai'i, http://moana.dnsalias.org). Opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. USDA is an equal opportunity provider and employer. NR 49 TC 2 Z9 2 U1 1 U2 6 PU AMER SOC PLANT TAXONOMISTS PI LARAMIE PA UNIV WYOMING, DEPT BOTANY 3165, 1000 E UNIVERSITY AVE, LARAMIE, WY 82071 USA SN 0363-6445 EI 1548-2324 J9 SYST BOT JI Syst. Bot. PD SEP PY 2015 VL 40 IS 3 BP 782 EP 790 DI 10.1600/036364415X689249 PG 9 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA CS2QO UT WOS:000361916400015 ER PT J AU Zhu, JY Chandra, MS Gleisner, R Gilles, W Gao, J Marrs, G Anderson, D Sessions, J AF Zhu, J. Y. Chandra, M. Subhosh Gleisner, Roland Gilles, William Gao, Johnway Marrs, Gevan Anderson, Dwight Sessions, John TI Case studies on sugar production from underutilized woody biomass using sulfite chemistry SO TAPPI JOURNAL LA English DT Article ID ENHANCE ENZYMATIC SACCHARIFICATION; CELLULOSIC ETHANOL-PRODUCTION; OVERCOME RECALCITRANCE; HARVEST RESIDUE; LODGEPOLE PINE; KNOT REJECTS; DILUTE-ACID; HIGH SOLIDS; HIGH-TITER; SPORL AB We examined two case studies to demonstrate the advantages of sulfite chemistry for pretreating underutilized woody biomass to produce sugars through enzymatic saccharification. In the first case study, we evaluated knot rejects from a magnesium-based sulfite mill for direct enzymatic sugar production. We found that the sulfite mill rejects are an excellent feedstock for sugar production. In the second study, we presented SPORL (sulfite pretreatment to overcome the recalcitrance of lignocelluloses), a sulfite pretreatment process based on modified sulfite pulping for robust bioconversion of softwood forest residues. Sulfite pulping technology is well developed, with proven commercial scalability, and sulfite pretreatment is a strong contender for commercial adoption. C1 [Zhu, J. Y.; Gleisner, Roland; Gilles, William] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Chandra, M. Subhosh] Yogi Vemana Univ, Dept Microbiol, Kadapa, India. [Marrs, Gevan; Anderson, Dwight] Weyerhaeuser Co, Federal Way, WA USA. [Sessions, John] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. RP Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us FU Agriculture and Food Research Initiative Competitive grant [2011-68005-30416]; USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA); University Grants Commission (UGC), New Delhi, India FX The authors are thankful for the financial support of the Agriculture and Food Research Initiative Competitive grant (No. 2011-68005-30416), USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA). The authors also thank Novozymes North America for providing the cellulase enzymes and Fred Matt of USFS-FPL for conducting the chemical composition analysis. Cosmo Specialty Fibers provided the sulfite mill rejects. Funding was also received from University Grants Commission (UGC), New Delhi, India for the visiting appointment of Dr. Chandra at the USDA Forest Products Laboratory. NR 27 TC 2 Z9 2 U1 1 U2 5 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD SEP PY 2015 VL 14 IS 9 BP 577 EP 583 PG 7 WC Materials Science, Paper & Wood SC Materials Science GA CS9RO UT WOS:000362429100003 ER PT J AU Jardim, CM Oliveira, RC Hsieh, JS Ragauskas, AJ Elder, T AF Jardim, C. M. Oliveira, R. C. Hsieh, J. S. Ragauskas, A. J. Elder, T. TI Eucalyptus fiber modification using dielectric-barrier discharge SO TAPPI JOURNAL LA English DT Article ID ATMOSPHERIC PLASMA ACTIVATION; SURFACE-PROPERTIES; WET-STRENGTH; KRAFT PULP; MECHANISM; QUALITY; CHARGE; AFM AB This study involves an investigation of the impact of the various levels of atmospheric dielectric-barrier discharge (DBD) treatment on the surface of eucalyptus fibers at different levels of beating with the objective of enhancing paper strength properties. The DBD treatment led to significant improvements in wet-strength tensile properties, increasing them by 2%-112%, as a function of the dosage of atmospheric plasma applied. Also, using controlled dosages of the DBD treatment, dry-strength tensile could be improved, reaching a 58% increase. In addition, non-beaten samples showed greater increases in strength properties than the beaten samples. Moreover, the acid surface content was unaltered by the DBD treatment, perhaps due to over-oxidation and generation of low molecular weight species that are easily removed by washing with water. Furthermore, increases in wettability could be observed for specific DBD dosage of treatments. Atomic force microscopy images of the treated handsheets showed that DBD treatment results in surface smoothing of the fibers. In conclusion, DBD treatment was shown to be an effective approach for eucalyptus fiber modification, especially in terms of enhancement of wet-strength properties. C1 [Jardim, C. M.] Univ Fed Vicosa, Forest Engn Dept, Vicosa, MG, Brazil. [Hsieh, J. S.] Georgia Inst Technol, Inst Paper & Sci Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA. [Ragauskas, A. J.] Univ Tennessee, Biorefining, Oak Ridge Natl Lab, Dept Chem & Biomol Engn,Dept Forestry, Knoxville, TN USA. [Elder, T.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA USA. RP Jardim, CM (reprint author), Univ Fed Vicosa, Forest Engn Dept, Vicosa, MG, Brazil. EM carolina.jardim@ufv.br FU CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazil FX The authors wish to acknowledge the support of the member companies of the Institute of Paper Science and Technology at the Georgia Institute of Technology. Also, the CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazil, for the scholarship to doctoral student Carolina M. Jardim. NR 26 TC 0 Z9 0 U1 3 U2 4 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD SEP PY 2015 VL 14 IS 9 BP 585 EP 591 PG 7 WC Materials Science, Paper & Wood SC Materials Science GA CS9RO UT WOS:000362429100004 ER PT J AU Levi, MR Schaap, MG Rasmussen, C AF Levi, Matthew R. Schaap, Marcel G. Rasmussen, Craig TI Application of Spatial Pedotransfer Functions to Understand Soil Modulation of Vegetation Response to Climate SO VADOSE ZONE JOURNAL LA English DT Article ID SATURATED HYDRAULIC CONDUCTIVITY; DIGITAL ELEVATION MODEL; WATER RETENTION; TERRAIN ATTRIBUTES; UNSATURATED SOILS; DESERT SOILS; REGRESSION; PREDICTION; FIELD; LANDFORM AB A fundamental knowledge gap in understanding land-atmosphere interactions is accurate, high-resolution spatial representation of soil physical and hydraulic properties. We present a novel approach to predict hydraulic soil parameters by combining digital soil mapping techniques with pedotransfer functions (PTFs) and demonstrate that simple derived quantities are related to observed spatial patterns in ecosystem production during the North American Monsoon. Landsat reflectance and elevation data were used to predict physical soil properties at a 5-m spatial resolution for a semiarid landscape of 6265 ha using regression kriging. Resulting soil property maps were applied to the Rosetta PTF to predict saturated hydraulic conductivity and water retention parameters from which approximate water residence times were derived. Estimated idealized residence time for water lost to the deeper vadose zone and evapotranspiration corresponded to vegetation response. Antecedent precipitation was more important for explaining the relationships between modeled soil properties and vegetation response than the amount of monsoon precipitation. Increased spring precipitation before the monsoon produced stronger negative correlations with hydraulic conductivity and stronger positive correlations with plant available water. Modeled water residence times explained the patterns of vegetation and landscape morphology validating our approach as a method of producing functional spatial PTFs. Linking digital soil mapping with Rosetta led to predictions of hydraulic soil properties that were more closely related to vegetation dynamics than the data available in the Soil Survey Geographic (SSURGO) soil database. C1 [Levi, Matthew R.] New Mexico State Univ, USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Schaap, Marcel G.; Rasmussen, Craig] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. RP Levi, MR (reprint author), New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. EM mrlevi21@email.arizona.edu FU USDA-NRCS of Arizona [68-9457-8-466]; NSF [EAR/IF 0929850]; Arizona Agricultural Experiment Station [ARZT-1367190-H21-155] FX This research was supported by the USDA-NRCS of Arizona cooperative agreement 68-9457-8-466, NSF EAR/IF 0929850, and the Arizona Agricultural Experiment Station ARZT-1367190-H21-155. We thank S. Loheide and two anonymous reviewers for providing comments that improved the quality of this work. NR 74 TC 1 Z9 1 U1 6 U2 15 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD SEP PY 2015 VL 14 IS 9 DI 10.2136/vzj2014.09.0126 PG 14 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CS4SH UT WOS:000362065700001 ER PT J AU Northup, BK Rao, SC AF Northup, Brian K. Rao, Srinivas C. TI Green Manures in Continuous Wheat Systems Affect Grain Yield and Nitrogen Content SO AGRONOMY JOURNAL LA English DT Article ID SOUTHERN GREAT-PLAINS; WARM-SEASON LEGUMES; WINTER-WHEAT; NUTRITIVE-VALUE; WATER-CONTENT; SPRING WHEAT; GRASS PEA; FORAGE; GROWTH; FALLOW AB Continuous winter wheat (Triticum aestivum L. em Thell.) is the foundation for most US Southern Great Plains (SGP) agriculture. Inorganic N fertilizers are important to wheat production, but increasing N prices have caused producers to reconsider growing legumes during summer fallow for green N. This study was conducted during 2008 to 2012 to determine the potential for using lablab [Lablab purpureus (L.) Sweet cv. Rio Verde] to support wheat under conventional and no-till management compared with soybean [Glycine max (L.) Merr. cv. Laredo] and three inorganic fertilizer treatments (none, 40, and 80 kg N ha(-1)). Legume seeds were inoculated and sown aft er wheat harvest each year, grown from June to August, and terminated in early September. Wheat was then sown with or without preplant tillage and grown to maturity. Grain yield, N concentration, and N accumulated in grain were analyzed to define N treatment, tillage system, and year effects. The amount and distribution of precipitation during 2008 to 2012 varied from 53 to 92% and 63 to 160% of the long-term averages for wheat (688 mm) and legume (162 mm) phases. Tillage effects were nonsignificant (P < 0.76), but N treatment x year interactions were significant for grain yield, N concentration, and N accumulated in grain (P < 0.01). The legumes resulted in some single-year increases in grain yield, but the overall yield response was inconsistent. The legume treatments reduced N concentration in wheat grain compared with the unfertilized control. These results show that neither legume was an effective short-term (<= 4-yr) N source for systems of continuous wheat production in the SGP. C1 [Northup, Brian K.; Rao, Srinivas C.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Northup, BK (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM brian.northup@ars.usda.gov NR 44 TC 3 Z9 3 U1 1 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD SEP-OCT PY 2015 VL 107 IS 5 BP 1666 EP 1672 DI 10.2134/agronj14.0428 PG 7 WC Agronomy SC Agriculture GA CS1NK UT WOS:000361832800004 ER PT J AU Williams, MM AF Williams, Martin M., II TI Identifying Crowding Stress-Tolerant Hybrids in Processing Sweet Corn SO AGRONOMY JOURNAL LA English DT Article ID ZEA-MAYS L.; POPULATION-DENSITY; PLANT-POPULATIONS; YIELD STABILITY; DIFFERENT ERAS; MAIZE; RESPONSES; NITROGEN AB Improvement in tolerance to intense competition at high plant populations (i.e., crowding stress) is a major genetic driver of corn (Zea mays L.) yield gain the last half-century. Recent research found differences in crowding stress tolerance among a few modern processing sweet corn hybrids; however, an investigation of interactions with other factors would reveal a deeper understanding of crowding stress tolerance in sweet corn. The objectives of this study were to (i) compare yield, recovery, and processor profitability of sweet corn hybrids grown under conditions of crowding stress, and (ii) determine if an interaction exists between N fertilization and hybrid on crop response to crowding stress. Twenty-six hybrids were grown under suboptimal and supraoptimal N fertilization at 72,000 plants ha(-1), a level beyond the optimal population of the most crowding stress-tolerant hybrid. Results showed hybrid and N fertilization had no interactive effect on key variables of interest to the sweet corn processing industry, namely green ear mass, recovery, case production, and gross profit margin. Therefore, hybrid rankings were consistent whether the crop was N stressed or not. Relative to the poorest performing hybrid, the highest performing hybrid grown at an elevated population yielded 50% more green ear mass, 61% greater case production, and 71% higher gross profit margin. This work demonstrated a simple method to identify processing sweet corn hybrids with the best tolerance to crowding stress. Significant gains in sweet corn productivity may be realized by growing such hybrids at plant populations higher than currently used. C1 Univ Illinois, USDA ARS, Global Change & Photosynth Res, Urbana, IL 61801 USA. RP Williams, MM (reprint author), Univ Illinois, USDA ARS, Global Change & Photosynth Res, Urbana, IL 61801 USA. EM martin.williams@ars.usda.gov FU Midwest Food Processors Association FX Nick Hausman, Jim Moody, and Ronnie Warsaw provided excellent technical guidance in conducting the field experiments. I wish to thank the many students who provided assistance. This research was made possible with support from the Midwest Food Processors Association. I greatly appreciate the participation of companies listed in Table 1. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Dep. of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 25 TC 2 Z9 2 U1 1 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD SEP-OCT PY 2015 VL 107 IS 5 BP 1782 EP 1788 DI 10.2134/agronj15.0011 PG 7 WC Agronomy SC Agriculture GA CS1NK UT WOS:000361832800017 ER PT J AU Sainju, UM Allen, BA Caesar-TonThat, T Lenssen, AW AF Sainju, Upendra M. Allen, Brett A. Caesar-TonThat, Thecan Lenssen, Andrew W. TI Dryland Soil Carbon and Nitrogen after Thirty Years of Tillage and Cropping Sequence Combination SO AGRONOMY JOURNAL LA English DT Article ID NORTHERN GREAT-PLAINS; LONG-TERM TILLAGE; ORGANIC-MATTER; WHEAT RESPONSE; SPRING WHEAT; INTENSITY; SYSTEM; STOCKS; SEQUESTRATION; FERTILIZATION AB Little is known about the long-term management impact on soil C and N contents in the northern Great Plains. We evaluated the 30-yr effect of tillage and cropping sequence combination on dryland crop biomass yield and soil bulk density, soil organic carbon (SOC), soil inorganic carbon (SIC), soil total nitrogen (STN), NH4-N, and NO3-N contents at the 0-to 120-cm depth in a Dooley sandy loam (fine loamy, mixed, frigid Typic Argiboroll) in eastern Montana. Treatments were no-till continuous spring wheat (Triticum aestivum L.) (NTCW), spring till continuous spring wheat (STCW), fall and spring till continuous spring wheat (FSTCW), fall and spring till spring wheat-barley (Hordeum vulgare L., 1984-1999) followed by spring wheat-pea (Pisum sativum L., 2000-2013) (FSTW-B/P), and spring till spring wheat-fallow (STW-F, traditional system). Mean annualized crop biomass returned to the soil was 23 to 30 % greater in NTCW, STCW, FSTCW, and FSTW-B/P than STW-F. At 0 to 7.5 cm, bulk density was 13 to 21% greater in STW-F, but SOC, SIC, and STN were 12 to 98% greater in STCW than other treatments. Ammonium-N and NO3-N contents were 25 to 74 % greater in FSTCW than other treatments. At other depths, SOC, SIC, STN, NH4-N and NO3-N contents varied among treatments. Reduced tillage with increased crop residue returned to the soil increased soil C and N storage in NTCW and STCW, but increased tillage intensity increased mineral N content in FSTCW compared with STW-F. Improved management practices, such as NTCW and STCW, may be adopted to improve dryland soil C and N stocks. C1 [Sainju, Upendra M.; Allen, Brett A.; Caesar-TonThat, Thecan] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Lenssen, Andrew W.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Sainju, UM (reprint author), USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov NR 41 TC 4 Z9 4 U1 2 U2 21 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD SEP-OCT PY 2015 VL 107 IS 5 BP 1822 EP 1830 DI 10.2134/agronj15.0106 PG 9 WC Agronomy SC Agriculture GA CS1NK UT WOS:000361832800021 ER PT J AU Thapa, R Chatterjee, A Johnson, JMF Awale, R AF Thapa, Resham Chatterjee, Amitava Johnson, Jane M. F. Awale, Rakesh TI Stabilized Nitrogen Fertilizers and Application Rate Influence Nitrogen Losses under Rainfed Spring Wheat SO AGRONOMY JOURNAL LA English DT Article ID FILLED PORE-SPACE; OXIDE EMISSIONS; N2O EMISSIONS; AMMONIA VOLATILIZATION; UREASE INHIBITOR; NITRIFICATION INHIBITORS; CROPPING SYSTEM; CARBON-DIOXIDE; WINTER-WHEAT; TILL CORN AB Nitrogen losses associated with fertilizer application have negative economic and environmental consequences, but urease and nitrification inhibitors have potential to reduce N losses. The effectiveness of these inhibitors has been studied extensively in irrigated but not in rainfed systems. Th is study was conducted at Glyndon, MN, under rainfed conditions to assess the impact of urease and nitrification inhibitors on NH3 volatilization, N2O emissions, and NO3- concentrations below the spring wheat (Triticum aestivum L.) rooting zone. Urea (U), urea with urease and nitrification inhibitors (SU), and urea with nitrification inhibitor only (UI) were applied at 146 and 168 kg N ha(-1) along with the control treatments. Cumulative NH3 volatilization was reduced by 26%, N2O emissions measured 18 d aft er planting were reduced by 50% with SU, but no significant reduction was observed with UI compared to U. We did not observe a significant effect of higher N rate on N2O emissions, but lower N application rate (146 kg N ha(-1)) significantly reduced NH3 volatilization by 26% compared to 168 kg N ha(-1). Nitrate concentration below the rooting zone was reduced by applying N at lower rate and also through the use of SU and UI instead of U. Soil inorganic N intensity was significantly related with cumulative N2O emissions. Nitrogen source and rate did not influence grain yield and protein content. Th is single-growing season study under rainfed conditions suggests that fertilizer N-stabilizers can be successfully used to minimize N losses without compromising grain yield and protein content. C1 [Thapa, Resham; Chatterjee, Amitava; Awale, Rakesh] N Dakota State Univ, Dept Soil Sci, NDSU Dept 7680, Fargo, ND 58108 USA. [Johnson, Jane M. F.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Thapa, R (reprint author), N Dakota State Univ, Dept Soil Sci, NDSU Dept 7680, POB 6050, Fargo, ND 58108 USA. EM resham.thapa@ndsu.edu OI Johnson, Jane/0000-0002-1687-4007 FU Koch Agronomic Services FX Authors wish to thank Koch Agronomic Services for partially funding this project and Dr. Abbey Wick for the graduate assistantship. We greatly appreciate the technical assistance provided by Norman Cattanach, Manbir Kaur Rakkar, Heidi Rasmussen, Cassey Nelson, and Spencer Nelson in maintaining the plots and sample collections. NR 56 TC 4 Z9 4 U1 6 U2 36 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD SEP-OCT PY 2015 VL 107 IS 5 BP 1885 EP 1894 DI 10.2134/agronj15.0081 PG 10 WC Agronomy SC Agriculture GA CS1NK UT WOS:000361832800028 ER PT J AU Baumhardt, RL Mauget, SA Gowda, PH Brauer, DK Marek, GW AF Baumhardt, R. L. Mauget, S. A. Gowda, P. H. Brauer, D. K. Marek, G. W. TI Optimizing Cotton Irrigation Strategies as Influenced by El Nino Southern Oscillation SO AGRONOMY JOURNAL LA English DT Article ID WATER-USE EFFICIENCY; HIGH-PLAINS; LINT YIELD; MANAGEMENT; US AB Equatorial Pacific sea surface temperature anomalies can cause a systematic El Nino-Southern Oscillation (ENSO) coupling with the atmosphere to produce predictable weather patterns in much of North America. Adapting irrigation strategies for drought-tolerant crops like cotton (Gossypium hirsutum L.) to exploit forecast climatic conditions represents one potential innovative technique for managing the declining Ogallala Aquifer beneath the US Southern High Plains. The crop simulation model GOSSYM was used with ENSO phase-specific weather records during 1959 to 2000 at Bushland, TX, to estimate lint yields of cotton emerging on three dates from soil at 50 or 75% available water content for all possible combinations of irrigation durations (0, 4, 6, 8, and 10 wk) and rates (2.5, 3.75, and 5.0 mm d(-1)). From those data, our objective was to compare partial center pivot deficit irrigation strategies that optimize calculated net cotton lint yield in relation to ENSO phase, initial soil water content, and emergence date. Although phase classification in June was inconsistent with maturing fall phases, the most accurately classified La Nina phase had limited rain that reduced lint yields compared with wetter Neutral and El Nino phases. During La Nina phase conditions, irrigation strategies that focused fixed water resources on smaller areas were better suited to increase net yield than spreading water across larger areas. Alternatively, during less predictable and wetter Neutral and El Nino phases, irrigation strategies that spread water increased net lint yield over focused applications except when both initial soil water and irrigation amount were limiting. C1 [Baumhardt, R. L.; Gowda, P. H.; Brauer, D. K.; Marek, G. W.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Mauget, S. A.] USDA ARS, Cropping Syst Res Lab, Lubbock, TX 79424 USA. RP Baumhardt, RL (reprint author), USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. EM r.louis.baumhardt@ars.usda.gov FU Ogallala Aquifer Program, a consortium of the USDA-ARS; Kansas State University; Texas A&M AgriLife Research; Texas A&M AgriLife Extension Service; Texas Tech University; West Texas AM University FX This research was supported in part by the Ogallala Aquifer Program, a consortium of the USDA-ARS, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 32 TC 2 Z9 2 U1 0 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD SEP-OCT PY 2015 VL 107 IS 5 BP 1895 EP 1904 DI 10.2134/agronj14.0471 PG 10 WC Agronomy SC Agriculture GA CS1NK UT WOS:000361832800029 ER PT J AU Hao, B Xue, Q Marek, TH Jessup, KE Becker, J Hou, X Xu, W Bynum, ED Bean, BW Colaizzi, PD Howell, TA AF Hao, B. Xue, Q. Marek, T. H. Jessup, K. E. Becker, J. Hou, X. Xu, W. Bynum, E. D. Bean, B. W. Colaizzi, P. D. Howell, T. A. TI Water Use and Grain Yield in Drought-Tolerant Corn in the Texas High Plains SO AGRONOMY JOURNAL LA English DT Article ID ZEA-MAYS L.; USE EFFICIENCY; DEFICIT IRRIGATION; STRESS TOLERANCE; UNITED-STATES; MAIZE; IMPROVEMENT; EVAPOTRANSPIRATION; BIOTECHNOLOGY; AGRICULTURE AB Drought is an important factor limiting corn (Zea mays L.) yields in the Texas High Plains, and adoption of drought-tolerant (DT) hybrids could be a management tool under water shortage. We conducted a 3-yr field study to investigate yield, evapotranspiration (ET), and water use efficiency (WUE) in DT hybrids. One conventional (33D49) and 4 DT hybrids (P1151HR, P1324HR, P1498HR, and P1564HR) were grown at three water regimes (I-100, I-75, and I-50, referring to 100, 75, and 50% ET requirement) and three planting densities (PD) (5.9, 7.4, and 8.4 plants m(-2)). Yield (13.56 Mg ha(-1)) and ET (719 mm) were the greatest at I-100 but WUE (2.1 kg m(-3)) was the greatest at I-75. Although DT hybrids did not always have greater yield and WUE than 33D49 at I-100, hybrids P1151HR and P1564HR consistently had greater yield and WUE than 33D49 at I-75 and I-50. Compared to 33D49, P1151HR and P1564HR had 8.6 to 12.1% and 19.1% greater yield at I-75 and I-50, respectively. Correspondingly, WUE was 9.8 to 11.7% and 20.0% greater at I-75 and I-50, respectively. Greater PD resulted in greater yield and WUE at I-100 and I-75 but PD did not affect yield and WUE at I-50. Yield and WUE in greater PD (8.4 plants m(-2)) were 6.3 to 8.3% greater than those in smaller PD (5.9 plants m(-2)). The results of this study demonstrated that proper selection of DT hybrids can increase corn yield and WUE under water-limited conditions. C1 [Hao, B.; Xue, Q.; Marek, T. H.; Jessup, K. E.; Hou, X.; Bynum, E. D.] Texas A&M AgriLife Res & Extens Ctr Amarillo, Amarillo, TX 79106 USA. [Xu, W.] Texas A&M AgriLife Res & Extens Ctr Lubbock, Lubbock, TX 79403 USA. [Becker, J.] Nextsteppe Seeds Inc, Hereford, TX 79045 USA. [Bean, B. W.] United Sorghum Checkoff, Lubbock, TX USA. [Colaizzi, P. D.; Howell, T. A.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. RP Xue, Q (reprint author), Texas A&M AgriLife Res & Extens Ctr Amarillo, 6500 Amarillo Blvd W, Amarillo, TX 79106 USA. EM qxue@ag.tamu.edu FU Texas A&M AgriLife Research Cropping System Program; Pioneer Hybrids International; USDA-Ogallala Aquifer Program; North Plains Groundwater Conservation District FX We are grateful to Texas A&M AgriLife Research staff Chance Reynolds, Brad Parker, Cole Pope, Bella Porras, and Bronc Finch for their help in field and laboratory work. This research was supported in part by Texas A&M AgriLife Research Cropping System Program, Pioneer Hybrids International, the USDA-Ogallala Aquifer Program, and North Plains Groundwater Conservation District. NR 57 TC 2 Z9 2 U1 1 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD SEP-OCT PY 2015 VL 107 IS 5 BP 1922 EP 1930 DI 10.2134/agronj15.0133 PG 9 WC Agronomy SC Agriculture GA CS1NK UT WOS:000361832800032 ER PT J AU Ott, JP Hartnett, DC AF Ott, Jacqueline P. Hartnett, David C. TI Bud-bank and tiller dynamics of co-occurring C-3 caespitose grasses in mixed-grass prairie SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE bud bank; caespitose; genet longevity; grasses; Hesperostipa comata; mixed-grass prairie; Nassella viridula; polycyclicity; tiller dynamics; vegetative reproduction ID TALLGRASS PRAIRIE; PERENNIAL GRASSES; LIFE-HISTORY; DEMOGRAPHY; GROWTH; COMMUNITIES; DEFOLIATION; PLANTS; PRODUCTIVITY; VIABILITY AB PREMISE OF THE STUDY: Tiller recruitment from the belowground bud bank of caespitose grasses influences their ability to monopolize local resources and, hence, their genet fitness. Differences in bud production and outgrowth among tiller types within a genet and among species may explain co-occurrence of caespitose grasses. This study aimed to characterize genet bud-bank and tiller production and dynamics in two co-occurring species and compare their vegetative reproductive strategies. METHODS: Bud-bank and tiller dynamics of Hesperostipa comata and Nassella viridula, dominant C-3 caespitose grasses in the northern mixed-grass prairie of North America, were assessed throughout an annual cycle. KEY RESULTS: The two species showed similar strategies, maintaining polycyclic tillers and thus creating mixed-age genet bud banks comprising multiple bud cohorts produced in different years. Vegetative tillers produced the majority of buds, whereas flowering tillers contributed little to the bud bank. Buds lived for at least 2 yr and were maintained in multiple developmental stages throughout the year. Because bud longevity rarely exceeded tiller longevity, tiller longevity drove turnover within the bud bank. Tiller population dynamics, more than bud production per tiller, determined the differential contribution of tiller types to the bud bank. Nassella viridula had higher bud production per tiller, a consistent annual tiller recruitment density, and greater longevity of buds on senesced and flowering tillers than H. comata. CONCLUSIONS: Co-occurring C-3 caespitose grasses had similar bud-bank and tiller dynamics contributing to genet persistence but differed in bud characteristics that could affect genet longevity and species coexistence. C1 [Ott, Jacqueline P.; Hartnett, David C.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. RP Ott, JP (reprint author), US Forest Serv, Forest & Grassland Res Lab, Rocky Mt Res Stn, 8221 S Highway 16, Rapid City, SD 57702 USA. EM jacquelinepott@fs.fed.us FU Kansas State University Division of Biology; Konza Prairie Long-Term Ecological Research Program; USDA Rangeland Research Program [310306] FX The authors thank C. Ferguson, J. Nippert, and L. Murray for helpful suggestions on the manuscript. We also thank K. Sebes for laboratory assistance and Wind Cave NP, especially B. Burkhart, for field-site support. This work was supported by the Kansas State University Division of Biology, the Konza Prairie Long-Term Ecological Research Program, and the USDA Rangeland Research Program (grant no. 310306). NR 46 TC 0 Z9 0 U1 6 U2 18 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 EI 1537-2197 J9 AM J BOT JI Am. J. Bot. PD SEP PY 2015 VL 102 IS 9 BP 1462 EP 1471 DI 10.3732/ajb.1500039 PG 10 WC Plant Sciences SC Plant Sciences GA CS0MH UT WOS:000361753900009 PM 26373977 ER PT J AU Gardner, EM Laricchia, KM Murphy, M Ragone, D Scheffler, BE Simpson, S Williams, EW Zerega, NJC AF Gardner, Elliot M. Laricchia, Kristen M. Murphy, Matthew Ragone, Diane Scheffler, Brian E. Simpson, Sheron Williams, Evelyn W. Zerega, Nyree J. C. TI CHLOROPLAST MICROSATELLITE MARKERS FOR ARTOCARPUS (MORACEAE) DEVELOPED FROM TRANSCRIPTOME SEQUENCES SO APPLICATIONS IN PLANT SCIENCES LA English DT Article DE Artocarpus altilis; Artocarpus camansi; Artocarpus mariannensis; Artocarpus odoratissimus; breadfruit; Moraceae ID DIVERSITY; PCR AB Premise of the study: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. Methods and Results: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis x A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy. Conclusions: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit. C1 [Gardner, Elliot M.; Laricchia, Kristen M.; Williams, Evelyn W.; Zerega, Nyree J. C.] Chicago Bot Garden, Dept Plant Sci, Glencoe, IL 60022 USA. [Gardner, Elliot M.; Laricchia, Kristen M.; Zerega, Nyree J. C.] Northwestern Univ, Plant Biol & Conservat, Evanston, IL 60208 USA. [Murphy, Matthew] Illinois Coll, Dept Biol, Jacksonville, IL 62650 USA. [Ragone, Diane] Natl Trop Bot Garden, Breadfruit Inst, Kahaleo, HI 96741 USA. [Scheffler, Brian E.; Simpson, Sheron] ARS, USDA, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. RP Gardner, EM (reprint author), Chicago Bot Garden, Dept Plant Sci, 1000 Lake Cook Rd, Glencoe, IL 60022 USA. EM egardner@u.northwestern.edu OI Scheffler, Brian/0000-0003-1968-8952 FU National Science Foundation [DEB REVSYS 0919119, DBI REU 1062675]; American Society of Plant Taxonomists; Garden Club of America; Northwestern University; USDA-ARS National Programs FX The authors thank the Sabah Forestry Department (J. Pereira), Sabah Agriculture Department (J. Linton, W. F. Au), and the Vanuatu Agricultural Research and Technical Center for coordinating fieldwork and providing access to living collections. This study was funded in part by the National Science Foundation (DEB REVSYS 0919119, DBI REU 1062675), the American Society of Plant Taxonomists, the Garden Club of America, Northwestern University, and USDA-ARS National Programs. NR 16 TC 4 Z9 4 U1 2 U2 8 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 2168-0450 J9 APPL PLANT SCI JI Appl. Plant Sci. PD SEP PY 2015 VL 3 IS 9 AR 1500049 DI 10.3732/apps.1500049 PG 6 WC Plant Sciences SC Plant Sciences GA CS1PI UT WOS:000361838800005 ER PT J AU Duerr, AE Miller, TA Duerr, KLC Lanzone, MJ Fesnock, A Katzner, TE AF Duerr, Adam E. Miller, Tricia A. Duerr, Kerri L. Cornell Lanzone, Michael J. Fesnock, Amy Katzner, Todd E. TI Landscape-scale distribution and density of raptor populations wintering in anthropogenic-dominated desert landscapes SO BIODIVERSITY AND CONSERVATION LA English DT Article DE Anthropogenic development; Desert Renewable Energy Conservation Plan (DRECP); Habitat associations; Mojave Desert; Predatory birds; Raptors; Sonoran Desert; Surveys ID WEST MOJAVE DESERT; SUBSIDIZED PREDATORS; CAPTURE-RECAPTURE; COMMON RAVENS; CALIFORNIA; HABITAT; CONSERVATION; SELECTION; MARK AB Anthropogenic development has great potential to affect fragile desert environments. Large-scale development of renewable energy infrastructure is planned for many desert ecosystems. Development plans should account for anthropogenic effects to distributions and abundance of rare or sensitive wildlife; however, baseline data on abundance and distribution of such wildlife are often lacking. We surveyed for predatory birds in the Sonoran and Mojave Deserts of southern California, USA, in an area designated for protection under the "Desert Renewable Energy Conservation Plan", to determine how these birds are distributed across the landscape and how this distribution is affected by existing development. We developed species-specific models of resight probability to adjust estimates of abundance and density of each individual common species. Second, we developed combined-species models of resight probability for common and rare species so that we could make use of sparse data on the latter. We determined that many common species, such as red-tailed hawks, loggerhead shrikes, and especially common ravens, are associated with human development and likely subsidized by human activity. Species-specific and combined-species models of resight probability performed similarly, although the former model type provided higher quality information. Comparing abundance estimates with past surveys in the Mojave Desert suggests numbers of predatory birds associated with human development have increased while other sensitive species not associated with development have decreased. This approach gave us information beyond what we would have collected by focusing either on common or rare species, thus it provides a low-cost framework for others conducting surveys in similar desert environments outside of California. C1 [Duerr, Adam E.; Miller, Tricia A.; Katzner, Todd E.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Duerr, Kerri L. Cornell] Westminster Coll, Dept Biol, New Wilmington, PA USA. [Lanzone, Michael J.] Cellular Tracking Technol LLC, Somerset, PA USA. [Fesnock, Amy] Calif State Off, Bur Land Management, Sacramento, CA USA. [Katzner, Todd E.] US Forest Serv, USDA, Timber & Watershed Lab, Parsons, WV USA. [Katzner, Todd E.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID USA. RP Duerr, AE (reprint author), W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. EM adam.duerr@mail.wvu.edu OI Katzner, Todd/0000-0003-4503-8435 FU Bureau of Land Management, California State Office FX The Bureau of Land Management, California State Office provided funding. This work is Scientific Article No. 3239 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown. We thank T. Esque and two anonymous reviewers for their comments that greatly helped to improve this manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 38 TC 3 Z9 3 U1 2 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3115 EI 1572-9710 J9 BIODIVERS CONSERV JI Biodivers. Conserv. PD SEP PY 2015 VL 24 IS 10 BP 2365 EP 2381 DI 10.1007/s10531-015-0916-6 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CR8KU UT WOS:000361601400002 ER PT J AU Plotto, A Baldwin, E Bai, JH Raithore, S Manthey, J Deterre, S Leclair, C AF Plotto, Anne Baldwin, Elizabeth Bai, Jinhe Raithore, Smita Manthey, John Deterre, Sophie Leclair, Clotilde TI Complexity of Off Flavor in Orange Juice Made from Fruit Affected by the Huanglongbing Disease SO CHEMICAL SENSES LA English DT Meeting Abstract CT 37th Annual Meeting of the Association-for-Chemoreception-Sciences (AChemS) CY APR 22-25, 2015 CL Bonita Springs, FL SP Assoc Chemorecept Sci C1 [Plotto, Anne; Baldwin, Elizabeth; Bai, Jinhe; Raithore, Smita; Manthey, John; Deterre, Sophie; Leclair, Clotilde] ARS, USDA, USHRL, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 3 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0379-864X EI 1464-3553 J9 CHEM SENSES JI Chem. Senses PD SEP PY 2015 VL 40 IS 7 MA 93 BP 594 EP 594 PG 1 WC Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology SC Behavioral Sciences; Food Science & Technology; Neurosciences & Neurology; Physiology GA CR7KM UT WOS:000361528400171 ER PT J AU Kurtzman, CP Mateo, RQ Kolecka, A Theelen, B Robert, V Boekhout, T AF Kurtzman, Cletus P. Mateo, Raquel Quintilla Kolecka, Anna Theelen, Bart Robert, Vincent Boekhout, Teun TI Advances in yeast systematics and phylogeny and their use as predictors of biotechnologically important metabolic pathways SO FEMS YEAST RESEARCH LA English DT Review DE yeasts; taxonomy; systematics; biotechnology; ecology ID DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; GRADIENT GEL-ELECTROPHORESIS; INTERNAL TRANSCRIBED SPACER; HUMAN PATHOGENIC YEASTS; IN-SITU HYBRIDIZATION; NUCLEIC-ACID PROBES; LARGE-SUBUNIT RDNA; MALDI-TOF MS; RAPID IDENTIFICATION AB Detection, identification and classification of yeasts have undergone a major transformation in the last decade and a half following application of gene sequence analyses and genome comparisons. Development of a database (barcode) of easily determined DNA sequences from domains 1 and 2 (D1/D2) of the nuclear large subunit rRNA gene and from ITS now permits many laboratories to identify species quickly and accurately, thus replacing the laborious and often inaccurate phenotypic tests previously used. Phylogenetic analysis of gene sequences is leading to a major revision of yeast systematics that will result in redefinition of nearly all genera. This new understanding of species relationships has prompted a change of rules for naming and classifying yeasts and other fungi, and these new rules are presented in the recently implemented International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). The use of molecular methods for species identification and the impact of Code changes on classification will be discussed, as will use of phylogeny for prediction of biotechnological applications. C1 [Kurtzman, Cletus P.] ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Mateo, Raquel Quintilla; Kolecka, Anna; Theelen, Bart; Robert, Vincent; Boekhout, Teun] CBS Fungal Biodivers Ctr CBS KNAW, NL-3584 CT Utrecht, Netherlands. [Mateo, Raquel Quintilla] Katholieke Univ Leuven, Inst Bot & Microbiol, Mol Cell Biol Lab, B-3001 Heverlee, Belgium. [Mateo, Raquel Quintilla] VIB, Dept Mol Microbiol, B-3001 Leuven Heverlee, Flanders, Belgium. RP Kurtzman, CP (reprint author), ARS, USDA, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov FU Qatar National Research Fund [5-298-3-086]; Marie Curie Initial Training Network CORNUCOPIA [264717] FX This publication was made possible by NPRP grant 5-298-3-086 from the Qatar National Research Fund (a member of the Qatar Foundation) to Teun Boekhout. The authors thank Marie Curie Initial Training Network CORNUCOPIA (FP7-PEOPLE- 2010-ITN, grant agreement nr. 264717) for the funding. NR 149 TC 6 Z9 6 U1 8 U2 21 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1567-1356 EI 1567-1364 J9 FEMS YEAST RES JI FEMS Yeast Res. PD SEP PY 2015 VL 15 IS 6 AR fov050 DI 10.1093/femsyr/fov050 PG 17 WC Biotechnology & Applied Microbiology; Microbiology; Mycology SC Biotechnology & Applied Microbiology; Microbiology; Mycology GA CS0ZT UT WOS:000361791700004 ER PT J AU Zaragoza, G Rose, JP Purcell, K Todd, BD AF Zaragoza, George Rose, Jonathan P. Purcell, Kathryn Todd, Brian D. TI Terrestrial Habitat use by Western Pond Turtles (Actinemys marmorata) in the Sierra Foothills SO JOURNAL OF HERPETOLOGY LA English DT Article ID FRESH-WATER TURTLES; WETLAND CONSERVATION; BUFFER ZONES; CALIFORNIA; AMPHIBIANS; PATTERNS AB Western Pond Turtles (Actinemys marmorata) are endemic to western North America and are found in a diversity of aquatic habitats. To date, few studies have examined the ecology of populations in ephemeral or intermittent ponds. Here, we studied the terrestrial habitat requirements of Western Pond Turtles in an intermittent pond that dries in years with below-average rainfall. We tracked terrestrial movements of Western Pond Turtles in an ephemeral pond at the San Joaquin Experimental Range in Madera County, California, USA, in the western foothills of the Sierra Nevada. We used radiotelemetry in 2012-13 to record their terrestrial locations and timing of departure from, and return to, the pond. Also, we examined the terrestrial microhabitat turtles selected for aestivation and overwintering. Turtles began leaving the pond as it dried in the late spring and early summer, spending an average of 235 d out of water, and their return to the pond was correlated with increasing rainfall during late winter. The majority of terrestrial locations were concealed completely in litter or duff and 95% of terrestrial locations during the study occurred within 187 m of the pond edge. Turtles in our study generally exhibited terrestrial habitat use similar to that of populations in intermittent lotic systems such as the many snow-melt and rain-fed rivers in northern California. Our results reinforce the importance of terrestrial habitat in the life history of Western Pond Turtles and the context-dependence of their habitat needs. C1 [Zaragoza, George; Rose, Jonathan P.; Todd, Brian D.] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [Purcell, Kathryn] US Forest Serv, USDA, Pacific Southwest Res Stn, Fresno, CA 93710 USA. RP Todd, BD (reprint author), Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, One Shields Ave, Davis, CA 95616 USA. EM btodd@ucdavis.edu RI Purcell, Kathryn/S-2592-2016 NR 25 TC 1 Z9 1 U1 5 U2 15 PU SOC STUDY AMPHIBIANS REPTILES PI ST LOUIS PA C/O ROBERT D ALDRIDGE, ST LOUIS UNIV, DEPT BIOLOGY, 3507 LACLEDE, ST LOUIS, MO 63103 USA SN 0022-1511 EI 1937-2418 J9 J HERPETOL JI J. Herpetol. PD SEP PY 2015 VL 49 IS 3 BP 437 EP 441 DI 10.1670/13-154 PG 5 WC Zoology SC Zoology GA CS1PZ UT WOS:000361840800016 ER PT J AU Hutcheson, HJ Mertins, JW Larson, SR Paskewitz, S AF Hutcheson, H. Joel Mertins, James W. Larson, Scott R. Paskewitz, Susan TI Acarine Hypopi (Sarcoptiformes: Acaridae) on Ixodes scapularis (Ixodida: Ixodidae) in Central North America SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE phoresy; blacklegged tick; heteromorphic deutonymph; Prostriata; Schwiebea nova ID SCHWIEBEA-OUDEMANS; ASTIGMATID MITES; COLEOPTERA AB Phoretic deutonymphs (hypopi) of Schwiebea sp. mites were detected during examinations of questing adult and nymphal blacklegged ticks, Ixodes scapularis Say, from vegetation at two woodland sites in northeastern Wisconsin, USA, during May and June, 2014. Collection sites were in Marinette and Oconto Counties, respectively. These hypopi are evidently the first reported from ticks in the Western Hemisphere, and they seem to be most similar morphologically to the putatively Holarctic species Schwiebea nova (Oudemans). C1 [Hutcheson, H. Joel; Mertins, James W.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Sci Technol & Anal Serv,Natl Vet Serv Labs, Ames, IA 50010 USA. [Larson, Scott R.; Paskewitz, Susan] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. RP Hutcheson, HJ (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Sci Technol & Anal Serv,Natl Vet Serv Labs, 1920 Dayton Ave, Ames, IA 50010 USA. EM hjoel.hutcheson@aphis.usda.gov NR 37 TC 0 Z9 0 U1 2 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD SEP PY 2015 VL 52 IS 5 BP 837 EP 841 DI 10.1093/jme/tjv085 PG 5 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CS0PS UT WOS:000361764900011 PM 26336225 ER PT J AU Ruder, MG Stallknecht, DE Howerth, EW Carter, DL Pfannenstiel, RS Allison, AB Mead, DG AF Ruder, Mark G. Stallknecht, David E. Howerth, Elizabeth W. Carter, Deborah L. Pfannenstiel, Robert S. Allison, Andrew B. Mead, Daniel G. TI Effect of Temperature on Replication of Epizootic Hemorrhagic Disease Viruses in Culicoides sonorensis (Diptera: Ceratopogonidae) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Culicoides; EHDV; epizootic hemorrhagic disease virus; temperature; vector ID WHITE-TAILED DEER; BLUETONGUE-VIRUS; VECTOR COMPETENCE; ORAL INFECTION; VARIIPENNIS DIPTERA; ODOCOILEUS-VIRGINIANUS; CLIMATE-CHANGE; UNITED-STATES; C-BOLITINOS; SEROTYPE 7 AB Replication of arboviruses, including orbiviruses, within the vector has been shown to be temperature dependent. Cooler ambient temperatures slow virus replication in arthropod vectors, whereas viruses replicate faster and to higher titers at warmer ambient temperatures. Previous research with epizootic hemorrhagic disease virus (EHDV) serotype 1 demonstrated that higher temperatures were associated with shorter extrinsic incubation periods in Culicoides sonorensis Wirth & Jones, a confirmed vector of EHDV in North America. To further our understanding of the effect of temperature on replication of EHDV within the vector, C. sonorensis were experimentally infected with one of three EHDV strains representing three serotypes (1, 2, and 7). Midges were fed defibrinated white-tailed deer (Odocoileus virginianus) blood spiked with EHDV (a parts per thousand yen10(6.5) TCID50/ml) through a parafilm membrane using an artificial feeding device and were then held at 20, 25, or 30A degrees C. In addition to this in vitro method, a white-tailed deer experimentally infected with EHDV-7 was used to provide an infectious bloodmeal to determine if the results were comparable with those from the in vitro feeding method. Whole midges were processed for virus isolation and titration at regular intervals following feeding; midges with a parts per thousand yen10(2.7) TCID50 were considered potentially competent to transmit virus. The virus recovery rates were high throughout the study and all three viruses replicated within C. sonorensis to high titer (a parts per thousand yen 10(2.7) TCID50/midge). Across all virus strains, the time to detection of potentially competent midges decreased with increasing temperature: 12-16 d postfeeding (dpf) at 20A degrees C, 4-6 dpf at 25A degrees C, and 2-4 dpf at 30A degrees C. Significant differences in replication of the three viruses in C. sonorensis were observed, with EHDV-2 replicating to a high titer in a smaller proportion of midges and with lower peak titers. The findings are consistent with previous studies of related orbiviruses, showing that increasing temperature can shorten the apparent extrinsic incubation period for multiple EHDV strains (endemic and exotic) in C. sonorensis. C1 [Ruder, Mark G.; Stallknecht, David E.; Allison, Andrew B.; Mead, Daniel G.] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Ruder, Mark G.; Howerth, Elizabeth W.; Carter, Deborah L.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. [Ruder, Mark G.; Pfannenstiel, Robert S.] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. RP Ruder, MG (reprint author), ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. EM mark.ruder@ars.usda.gov OI Allison, Andrew B./0000-0003-0971-1215 FU Safari Club International Foundation; United States - Israel Binational Agricultural Research and Development Fund [IS-4105-08] FX This research was funded by Safari Club International Foundation and the United States - Israel Binational Agricultural Research and Development Fund (research grant no. IS-4105-08). We thank Jim Kempert and William Yarnell of ABADRU for providing midges for use in these trials, and gratefully acknowledge the University of Georgia's Department of Entomology for use of their facilities during this work. We gratefully acknowledge the continued support of SCWDS member agencies. We thank David Osborn, Karl Miller, and Robert Warren of the Warnell School of Forestry and Natural Resources, University of Georgia, for providing white-tailed deer. We would like to than Dr. Velizar Bumbarov from the Kimron Veterinary Institute in Israel for isolating the EHDV-7 in Israel. NR 59 TC 0 Z9 0 U1 4 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD SEP PY 2015 VL 52 IS 5 BP 1050 EP 1059 DI 10.1093/jme/tjv062 PG 10 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CS0PS UT WOS:000361764900037 PM 26336204 ER PT J AU Turell, MJ Britch, SC Aldridge, RL Xue, RD Smith, ML Cohnstaedt, LW Linthicum, KJ AF Turell, Michael J. Britch, Seth C. Aldridge, Robert L. Xue, Rui-De Smith, Mike L. Cohnstaedt, Lee W. Linthicum, Kenneth J. TI Potential for Psorophora columbiae and Psorophora ciliata Mosquitoes (Diptera: Culicidae) to Transmit Rift Valley Fever Virus SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE competence; vector; transmission; North America ID NORTH-AMERICAN MOSQUITOS; SAUDI-ARABIA; CULEX-PIPIENS; VECTOR COMPETENCE; UNITED-STATES; WEST-AFRICA; EPIDEMIC; DISSEMINATION; OUTBREAK; EGYPT AB Rift Valley fever virus (RVFV) continues to pose a threat to much of the world. Unlike many arboviruses, numerous mosquito species have been associated with RVFV in nature, and many species have been demonstrated as competent vectors in the laboratory. In this study, we evaluated two field-collected Psorophora species, Psorophora columbiae (Dyar and Knab) and Psorophora ciliata (F.) for their potential to transmit RVFV in North America. Both species were susceptible to infection after feeding on a hamster with a viremia of 10(7) plaque-forming units/ml, with infection rates of 65 and 83% for Ps. columbiae and Ps. ciliata, respectively (with nearly all specimens becoming infected when feeding on a hamster with a higher viremia). However, both species had a significant salivary gland barrier, as only 2/35 Ps. columbiae and 0/3 Ps. ciliata with a disseminated infection transmitted virus by bite. Despite the presence of the salivary gland barrier, due to the very high population that can occur and its propensity to feed on large mammals, Ps. columbiae might play a role in amplifying RVFV should that virus be introduced into an area where this species is common. C1 [Turell, Michael J.] US Army Med Res Inst Infect Dis, Div Virol, Dept Vector Assessment, Ft Detrick, MD 21702 USA. [Britch, Seth C.; Aldridge, Robert L.; Linthicum, Kenneth J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Xue, Rui-De; Smith, Mike L.] Anastasia Mosquito Control Dist St Johns Cty, St Augustine, FL 32080 USA. [Cohnstaedt, Lee W.] USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Turell, MJ (reprint author), US Army Med Res Inst Infect Dis, Div Virol, Dept Vector Assessment, Ft Detrick, MD 21702 USA. EM michael.j.turell.civ@mail.mil FU U.S. Department of Homeland Security Science and Technology Directorate [HSHQDC-11-X-00326] FX We thank Jeremy Wittie, Gregory White, Melissa Snelling, and Arturo Gutierrez (Coachella Valley Mosquito and Vector Control District, Indio, CA) for providing the Psorophora specimens from California. Their expert support of mosquito collections and their handling and shipping of live specimens from field sites in the Coachella Valley, CA, were critical to this study. We thank S. Padilla, S. Pisarcik, and J. Hinson (USAMRIID) for processing and testing mosquito specimens; and J. Williams (USAMRIID) for caring for the hamsters. This work was supported by a grant HSHQDC-11-X-00326 from the U.S. Department of Homeland Security Science and Technology Directorate. NR 45 TC 3 Z9 3 U1 0 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD SEP PY 2015 VL 52 IS 5 BP 1111 EP 1116 DI 10.1093/jme/tjv093 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CS0PS UT WOS:000361764900045 PM 26336233 ER PT J AU Hoel, DF Dunford, JC Kline, DL Irish, SR Weber, M Richardson, AG Doud, CW Wirtz, RA AF Hoel, David F. Dunford, James C. Kline, Daniel L. Irish, Seth R. Weber, Michael Richardson, Alec G. Doud, Carl W. Wirtz, Robert A. TI A COMPARISON OF CARBON DIOXIDE SOURCES FOR MOSQUITO CAPTURE IN CENTERS FOR DISEASE CONTROL AND PREVENTION LIGHT TRAPS ON THE FLORIDA GULF COAST SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Carbon dioxide; generator; dry ice; citric acid; sodium bicarbonate; yeast ID L-LACTIC ACID; ANOPHELES-GAMBIAE; AEDES-AEGYPTI; BITING FLIES; 1-OCTEN-3-OL; ATTRACTANTS; YEAST; CULICIDAE; DIPTERA; BLENDS AB Traditional sources of carbon dioxide (CO2), dry ice, and compressed gas, were tested against 3 combinations of food-grade reagents known to generate CO2 using a compact, lightweight generator delivery system with Centers for Disease Control and Prevention light traps. Three 6 3 6 Latin square trials were completed near the Florida Gulf Coast in the Lower Suwannee Wildlife Refuge during the summer of 2013, collecting a total of 31,632 female mosquitoes. Treatments included dry ice, compressed CO2 gas, a control trap (no CO2), citric acid + sodium bicarbonate, vinegar + sodium bicarbonate, and yeast +/- sugar. Decreasing order of trap collections (treatment mean number of mosquitoes per trap night 6 standard error) were dry ice 773.5 (+/- 110.1). compressed gas 440.7 (+/- 42.3) > citric acid + sodium bicarbonate 197.6 (+/- 30.4), yeast + sugar 153.6 (+/- 27.4) > vinegar + sodium bicarbonate 109.6 (+/- 16.2) > control 82.4 (+/- 14.0). A 2-way Kruskal-Wallis analysis by treatment, site, and treatment 3 site interaction identified significant differences between all treatments. Although dry ice and compressed CO2 gas collected significantly more mosquitoes than other combinations (P < 0.05), use of citric acid and sodium bicarbonate or yeast and sugar greatly outperformed unbaited traps and offer a good alternative to dry ice and compressed gas in areas where these agents are not readily available or are difficult to obtain due to logistical constraints. An inexpensive, portable CO2 generator for use with food-grade reagents is described. C1 [Hoel, David F.] Ctr Dis Control & Prevent, Navy & Marine Corps Publ Hlth Ctr Detachment, Atlanta, GA 30329 USA. [Hoel, David F.] Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. [Dunford, James C.] US Navy Environm & Prevent Med, Norfolk, VA 23511 USA. [Kline, Daniel L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Irish, Seth R.; Wirtz, Robert A.] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA. [Weber, Michael] OnVector Technol, Sunnyvale, CA 94087 USA. [Richardson, Alec G.; Doud, Carl W.] Navy Entomol Ctr Excellence, Naval Air Stn Jacksonville, Jacksonville, FL 32212 USA. RP Hoel, DF (reprint author), Ctr Dis Control & Prevent, Navy & Marine Corps Publ Hlth Ctr Detachment, 1600 Clifton Rd, Atlanta, GA 30329 USA. NR 31 TC 1 Z9 1 U1 0 U2 6 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD SEP PY 2015 VL 31 IS 3 BP 248 EP 257 PG 10 WC Entomology SC Entomology GA CR7VZ UT WOS:000361561200006 PM 26375906 ER PT J AU Teske, ME Thistle, HW Bonds, JAS AF Teske, Milton E. Thistle, Harold W. Bonds, Jane A. S. TI A TECHNICAL REVIEW OF MULV-DISP, A RECENT MOSQUITO ULTRA-LOW VOLUME PESTICIDE SPRAY DISPERSION MODEL SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE AGDISP; deposition; MULV-Disp; spray gun; ultra-low volume ID AERIAL APPLICATIONS; DRIFT; DEPOSITION; MANAGEMENT; SIMULATION; DENSITY; AGDISP AB The authors of a recently published paper summarized the development of a regression model for ground-based ultra-low volume applications, suggesting that their model was sufficiently verified that it could be used extensively for mosquito control. These authors claimed that their statistical model was superior in its predictive capability to the extensively developed and Environmental Protection Agency-validated AGDISP mechanistic model. In this technical review, the assumptions, reduction and interpretation of data, and conclusions reached with regard to their model are discussed, and explicit misstatements and incorrect mathematical relationships are pointed out. Two published versions of the model regression equation give substantially different results without explanation. Petri dish collection was used for very small droplets, with no mention of collection efficiency. Meteorological data were misused based on manufacturer's specification of instrument accuracy. We strongly disagree with many of the model results and show that the model misrepresents the actual behavior of aerosol sprays applied in the manner tested. C1 [Teske, Milton E.] Continuum Dynam Inc, Ewing, NJ 08618 USA. [Thistle, Harold W.] USDA Forest Serv, Morgantown, WV 26505 USA. [Bonds, Jane A. S.] Bonds Consulting Grp, Panama City Beach, FL 32408 USA. RP Teske, ME (reprint author), Continuum Dynam Inc, 34 Lexington Ave, Ewing, NJ 08618 USA. NR 30 TC 2 Z9 2 U1 1 U2 2 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD SEP PY 2015 VL 31 IS 3 BP 262 EP 270 PG 9 WC Entomology SC Entomology GA CR7VZ UT WOS:000361561200008 PM 26375908 ER PT J AU Aldridge, RL Britch, SC Snelling, M Gutierez, A White, G Linthicum, KJ AF Aldridge, Robert L. Britch, Seth C. Snelling, Melissa Gutierez, Arturo White, Gregory Linthicum, Kenneth J. TI PASSIVE BAITED SEQUENTIAL FILTH FLY TRAP SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Adult fly control; adult fly surveillance; diurnal activity; Musca domestica ID FLIES; DIPTERA; MUSCA; BIONOMICS; MUSCIDAE AB Filth fly control measures may be optimized with a better understanding of fly population dynamics measured throughout the day. We describe the modification of a commercial motorized sequential mosquito trap to accept liquid odorous bait and leverage a classic inverted-cone design to passively confine flies in 8 modified collection bottles corresponding to 8 intervals. Efficacy trials in a hot-arid desert environment indicate no significant difference (P = 0.896) between the modified sequential trap and a Rid-Max (R) fly trap. C1 [Aldridge, Robert L.; Britch, Seth C.; Linthicum, Kenneth J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Snelling, Melissa; Gutierez, Arturo; White, Gregory] Coachella Valley Mosquito & Vector Control Dist, Indio, CA 92201 USA. RP Aldridge, RL (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. NR 15 TC 0 Z9 0 U1 0 U2 2 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD SEP PY 2015 VL 31 IS 3 BP 278 EP 282 PG 5 WC Entomology SC Entomology GA CR7VZ UT WOS:000361561200011 PM 26375911 ER EF