FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Lu, Y Jia, CJ Zhang, GL Zhao, YG Wilson, MA AF Lu, Ying Jia, Chongjian Zhang, Ganlin Zhao, Yuguo Wilson, Michael A. TI Spatial distribution and source of potential toxic elements (PTEs) in urban soils of Guangzhou, China SO ENVIRONMENTAL EARTH SCIENCES LA English DT Article DE Multivariate analysis; Geostatistical analysis; Potential toxic elements (PTEs); Spatial distribution maps; Source identification; Urban soil ID HEAVY-METAL CONTAMINATION; GIS-BASED APPROACH; GEOCHEMICAL SURVEY; INDUSTRIAL-CITY; SOURCE IDENTIFICATION; EUROPEAN CITIES; TRACE-ELEMENTS; NEW-ORLEANS; HONG-KONG; LAND USES AB Potential toxic elements (PTEs) in urban soils are of great environmental concern because of their potential long-term effects on human health. A systematic soil geochemical survey was performed across central urban districts of Guangzhou, the largest city in southern China. Surface soil samples (0-10 cm) were collected from 426 sites in order to evaluate the environmental quality of this urban area. Geostatistical and multivariate analysis (principal component, clustering, and correlation) were applied to generate spatial distribution maps of PTEs concentrations as well as identify possible sources of metals. The results showed that PTEs ranged widely in value; Arsenic, Hg, Pb, Cu Zn, Cd, Ni, and Mn ranged from 1.4 to 144, 0.01 to 12.2, 18.5 to 4903, 5.0 to 417, 10.1 to 1795, 0.03 to 2.41, 2.5 to 77.6, and 21.2 to 1286 mg/kg, respectively. Iron ranged from 6.1 to 61.8 g/kg. Soils were enriched with Cd, Cu, Hg, Mn, Pb and Zn as compared with soil background values of Guangzhou. Spatial distribution maps indicated similar distribution patterns of Cd, Cu, Pb and Zn. Urbanization history and industrial activities affected the accumulation of PTEs. Relatively higher concentrations of As, Cd, Cu, Hg, Pb and Zn were found in districts with a longer history of urbanization and industrial activities, such as the Liwan district along the Pearl River in western Guangzhou. This area was the most highly contaminated, whereas the Tianhe, a district with a limited time of development, was the least contaminated. Multi-variate statistical analysis showed distinctly different associations among the studied metals; suggesting that Fe, Ni and Mn were predominantly derived from a natural (geological) source; As, Cu, Hg, Pb and Zn from anthropogenic sources; and Cd from both sources. Present PTEs levels were also compared with those reported from other urban areas around world, and results indicate that further studies on assessing both the human and ecosystem risks associated with urban contaminated soils will be necessary. C1 [Lu, Ying; Jia, Chongjian] South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Ganlin; Zhao, Yuguo] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. [Wilson, Michael A.] USDA, Nat Resources Conservat Serv, Natl Soil Survey Ctr, Lincoln, NE USA. RP Lu, Y (reprint author), South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China.; Zhang, GL (reprint author), Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. EM luying@scau.edu.cn; glzhang@issas.ac.cn FU National Natural Science Foundation of China [41271233]; Department of Sciences and Technology of Guangdong Province [2013B020310009, 2014B020206002] FX This research was financially supported by the National Natural Science Foundation of China (Grant No. 41271233), and the Department of Sciences and Technology of Guangdong Province (Grant Nos. 2013B020310009, 2014B020206002). NR 80 TC 1 Z9 1 U1 10 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1866-6280 EI 1866-6299 J9 ENVIRON EARTH SCI JI Environ. Earth Sci. PD FEB PY 2016 VL 75 IS 4 AR 329 DI 10.1007/s12665-015-5190-0 PG 15 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA DF5LO UT WOS:000371393400051 ER PT J AU Ibekwe, AM Murinda, SE DebRoy, C Reddy, GB AF Ibekwe, A. M. Murinda, Shelton E. DebRoy, Chitrita Reddy, Gudigopura B. TI Potential pathogens, antimicrobial patterns and genotypic diversity of Escherichia coli isolates in constructed wetlands treating swine wastewater SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE constructed wetland; contaminants; E. coli; BOX AIR-1; antimicrobial ID MARSH-POND-MARSH; ANTIBIOTIC-RESISTANCE GENES; MOLECULAR SIGNATURES; POPULATION-STRUCTURE; FECAL CONTAMINATION; UNITED-STATES; SURFACE-WATER; FOOD ANIMALS; BACTERIA; CANADA AB Escherichia coli populations originating from swine houses through constructed wetlands were analyzed for potential pathogens, antimicrobial susceptibility patterns, and genotypic diversity. Escherichia coli isolates (n = 493) were screened for the presence of the following virulence genes: stx1, stx2 and eae (Shiga toxin-producing E. coli [STEC]), heat-labile enterotoxin (LT) genes and heat stable toxin STa and STb (enterotoxigenic E. coli (ETEC), cytotoxin necrotizing factors 1 and 2 (cnf1 and cnf2 [necrotoxigenic E. coli-NTEC]), as well as O and H antigens, and the presence of the antibiotic resistance genes bla(TEM), bla(SHV), bla(CMY-2), tet A, tet B, tet C, mph(A), aadA, StrA/B, sul1, sul2 and sul3. The commensal strains were further screened for 16 antimicrobials and characterized by BOX AIR-1 PCR for unique genotypes. The highest antibiotic resistance prevalence was for tetracycline, followed by erythromycin, ampicillin, streptomycin, sulfisoxazole and kanamycin. Our data showed that most of the isolates had high distribution of single or multidrug-resistant (MDR) genotypes. Therefore, the occurrence of MDR E. coli in the wetland is a matter of great concern due to possible transfer of resistance genes from nonpathogenic to pathogenic strains or vice versa in the environment. C1 [Ibekwe, A. M.] USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Murinda, Shelton E.] Calif State Polytech Univ Pomona, Dept Vet & Anim Sci, Pomona, CA 91768 USA. [DebRoy, Chitrita] Penn State Univ, E Coli Reference Ctr, State Coll, PA 16802 USA. [Reddy, Gudigopura B.] N Carolina Agr & Tech State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA. RP Ibekwe, AM (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Mark.Ibekwe@ars.usda.gov FU 214 Manure and Byproduct Utilization Project of the USDA-ARS FX This research was supported by the 214 Manure and Byproduct Utilization Project of the USDA-ARS. Mention of trademark or proprietary products in this manuscript does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 65 TC 2 Z9 2 U1 11 U2 26 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0168-6496 EI 1574-6941 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD FEB PY 2016 VL 92 IS 2 AR fiw006 DI 10.1093/femsec/fiw006 PG 14 WC Microbiology SC Microbiology GA DF3MX UT WOS:000371249600020 ER PT J AU Baranowski, T Blumberg, F Buday, R DeSmet, A Fiellin, LE Green, CS Kato, PM Lu, AS Maloney, AE Mellecker, R Morrill, BA Peng, W Shegog, R Simons, M Staiano, AE Thompson, D Young, K AF Baranowski, Tom Blumberg, Fran Buday, Richard DeSmet, Ann Fiellin, Lynn E. Green, C. Shawn Kato, Pamela M. Lu, Amy Shirong Maloney, Ann E. Mellecker, Robin Morrill, Brooke A. Peng, Wei Shegog, Ross Simons, Monique Staiano, Amanda E. Thompson, Debbe Young, Kimberly CA Inst Digital Media & Child Dev Wor TI Games for Health for Children-Current Status and Needed Research SO GAMES FOR HEALTH JOURNAL LA English DT Article ID ACTIVE VIDEO GAMES; PHYSICAL-ACTIVITY INTERVENTIONS; CHILDHOOD OBESITY PREVENTION; WEIGHT MANAGEMENT PROGRAM; BEHAVIOR-CHANGE; SERIOUS GAMES; EXECUTIVE FUNCTION; ELEMENTARY-SCHOOL; RANDOMIZED-TRIAL; AEROBIC FITNESS AB Videogames for health (G4H) offer exciting, innovative, potentially highly effective methods for increasing knowledge, delivering persuasive messages, changing behaviors, and influencing health outcomes. Although early outcome results are promising, additional research is needed to determine the game design and behavior change procedures that best promote G4H effectiveness and to identify and minimize possible adverse effects. Guidelines for ideal use of different types of G4H by children and adolescents should be elucidated to enhance effectiveness and minimize adverse effects. G4H stakeholders include organizational implementers, policy makers, players and their families, researchers, designers, retailers, and publishers. All stakeholders should be involved in G4H development and have a voice in setting goals to capitalize on their insights to enhance effectiveness and use of the game. In the future, multiple targeted G4H should be available to meet a population's diverse health needs in developmentally appropriate ways. Substantial, consistent, and sophisticated research with appropriate levels of funding is needed to realize the benefits of G4H. C1 [Baranowski, Tom; Thompson, Debbe] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Blumberg, Fran] Fordham Univ, Grad Sch Educ, Div Psychol & Educ Serv, New York, NY 10023 USA. [Buday, Richard] Archimage Inc, Houston, TX USA. [DeSmet, Ann] Univ Ghent, Dept Movement & Sport Sci, B-9000 Ghent, Belgium. [Fiellin, Lynn E.] Yale Univ, Sch Med, Dept Internal Med, play2PREVENT Lab, New Haven, CT 06510 USA. [Green, C. Shawn] Univ Wisconsin, Dept Psychol, 1202 W Johnson St, Madison, WI 53706 USA. [Kato, Pamela M.] Coventry Univ, Serious Games Inst, Coventry, W Midlands, England. [Lu, Amy Shirong] Northeastern Univ, Dept Commun Studies, Boston, MA 02115 USA. [Lu, Amy Shirong] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. [Maloney, Ann E.] Univ Massachusetts, Sch Med, Dept Psychiat, Worcester, MA 01655 USA. [Mellecker, Robin] Deakin Univ, Ctr Phys Act & Nutr Res, Sch Exercise & Nutr Sci, Fac Hlth, Melbourne, Vic, Australia. [Morrill, Brooke A.] Schell Games, Pittsburgh, PA USA. [Peng, Wei] Michigan State Univ, Dept Telecommun Informat Studies & Media, E Lansing, MI 48824 USA. [Shegog, Ross] Univ Texas Houston, Sch Publ Hlth, Ctr Hlth Promot & Prevent Res, Houston, TX USA. [Simons, Monique] Univ Utrecht, Dept Human Geog & Spatial Planning, Utrecht, Netherlands. [Staiano, Amanda E.] Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Young, Kimberly] St Bonaventure Univ, Russell J Jandoli Sch Journalism & Mass Commun, New York, NY USA. RP Baranowski, T (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Kato, Pamela/0000-0002-4755-5009; Baranowski, Tom/0000-0002-0653-2222; Lu, Amy Shirong/0000-0002-8230-9049; Peng, Wei/0000-0003-1576-4532 FU NCI NIH HHS [R21 CA158917]; NIDDK NIH HHS [R01 DK091254]; NIGMS NIH HHS [U54 GM104940] NR 161 TC 4 Z9 4 U1 4 U2 16 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD FEB 1 PY 2016 VL 5 IS 1 BP 1 EP 12 DI 10.1089/g4h.2015.0026 PG 12 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA DE7US UT WOS:000370842800001 PM 26262772 ER PT J AU Carlsen, M Fu, GF Bushman, S Corcoran, C AF Carlsen, Michelle Fu, Guifang Bushman, Shaun Corcoran, Christopher TI Exploiting Linkage Disequilibrium for Ultrahigh-Dimensional Genome-Wide Data with an Integrated Statistical Approach SO GENETICS LA English DT Article DE GWAS; linkage disequilibrium; feature screening; large-scale modeling; case-control; genomic selection; GenPred; shared data resource ID RIDGE-REGRESSION; COMPLEX DISEASES; HAPLOTYPE DIVERSITY; VARIABLE SELECTION; CANDIDATE GENES; ASSOCIATION; MODELS; LOCI; REVEALS; ALLELES AB Genome-wide data with millions of single-nucleotide polymorphisms (SNPs) can be highly correlated due to linkage disequilibrium (LD). The ultrahigh dimensionality of big data brings unprecedented challenges to statistical modeling such as noise accumulation, the curse of dimensionality, computational burden, spurious correlations, and a processing and storing bottleneck. The traditional statistical approaches lose their power due to p >> n (n is the number of observations and p is the number of SNPs) and the complex correlation structure among SNPs. In this article, we propose an integrated distance correlation ridge regression (DCRR) approach to accommodate the ultrahigh dimensionality, joint polygenic effects of multiple loci, and the complex LD structures. Initially, a distance correlation (DC) screening approach is used to extensively remove noise, after which LD structure is addressed using a ridge penalized multiple logistic regression (LRR) model. The false discovery rate, true positive discovery rate, and computational cost were simultaneously assessed through a large number of simulations. A binary trait of Arabidopsis thaliana, the hypersensitive response to the bacterial elicitor AvrRpm1, was analyzed in 84 inbred lines (28 susceptibilities and 56 resistances) with 216,130 SNPs. Compared to previous SNP discovery methods implemented on the same data set, the DCRR approach successfully detected the causative SNP while dramatically reducing spurious associations and computational time. C1 [Carlsen, Michelle; Fu, Guifang; Corcoran, Christopher] Utah State Univ, Dept Math & Stat, 3900 Old Main Hill, Logan, UT 84322 USA. [Bushman, Shaun] ARS, Forage & Range Res Lab, USDA, Logan, UT 84322 USA. RP Fu, GF (reprint author), Utah State Univ, Dept Math & Stat, 3900 Old Main Hill, Logan, UT 84322 USA. EM Guifang.fu@usu.edu OI Fu, Guifang/0000-0001-7896-7797 FU NFS [DMS - 1413366]; National Science Foundation [DMS-1413366] FX This work was mainly supported by a startup grant to G.F. (NFS DMS - 1413366) and also partially by a grant from the National Science Foundation (DMS-1413366) to G.F. (http://www.nsf.gov). The authors declare that there is no conflict of interest. NR 87 TC 0 Z9 0 U1 1 U2 4 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD FEB PY 2016 VL 202 IS 2 BP 411 EP 426 DI 10.1534/genetics.115.179507 PG 16 WC Genetics & Heredity SC Genetics & Heredity GA DF4FY UT WOS:000371304600008 PM 26661113 ER PT J AU Khalyani, AH Gould, WA Harmsen, E Terando, A Quinones, M Collazo, JA AF Khalyani, Azad Henareh Gould, William A. Harmsen, Eric Terando, Adam Quinones, Maya Collazo, Jaime A. TI Climate Change Implications for Tropical Islands: Interpolating and Interpreting Statistically Downscaled GCM Projections for Management and Planning* SO JOURNAL OF APPLIED METEOROLOGY AND CLIMATOLOGY LA English DT Article DE Classification; Tropics; Ensembles; General circulation models; Interpolation schemes; Evapotranspiration ID UNITED-STATES; PUERTO-RICO; POTENTIAL IMPACTS; LIFE ZONES; ENERGY USE; DRY DAYS; PRECIPITATION; TEMPERATURE; EVAPOTRANSPIRATION; CHINA AB The potential ecological and economic effects of climate change for tropical islands were studied using output from 12 statistically downscaled general circulation models (GCMs) taking Puerto Rico as a test case. Two model selection/model averaging strategies were used: the average of all available GCMs and the average of the models that are able to reproduce the observed large-scale dynamics that control precipitation over the Caribbean. Five island-wide and multidecadal averages of daily precipitation and temperature were estimated by way of a climatology-informed interpolation of the site-specific downscaled climate model output. Annual cooling degree-days (CDD) were calculated as a proxy index for air-conditioning energy demand, and two measures of annual no-rainfall days were used as drought indices. Holdridge life zone classification was used to map the possible ecological effects of climate change. Precipitation is predicted to decline in both model ensembles, but the decrease was more severe in the "regionally consistent" models. The precipitation declines cause gradual and linear increases in drought intensity and extremes. The warming from the 1960-90 period to the 2071-99 period was 4.6 degrees-9 degrees C depending on the global emission scenarios and location. This warming may cause increases in CDD, and consequently increasing energy demands. Life zones may shift from wetter to drier zones with the possibility of losing most, if not all, of the subtropical rain forests and extinction risks to rain forest specialists or obligates. C1 [Khalyani, Azad Henareh; Gould, William A.] US Forest Serv, USDA, Int Inst Trop Forestry, 1201 Calle Ceiba, San Juan, PR 00926 USA. [Khalyani, Azad Henareh; Quinones, Maya; Collazo, Jaime A.] N Carolina State Univ, Dept Appl Ecol, North Carolina Cooperat Fish & Wildlife Res Unit, Raleigh, NC 27695 USA. [Harmsen, Eric] Univ Puerto Rico, Dept Agr & Biosyst Engn, Mayaguez, PR USA. [Terando, Adam] US Geol Survey, Southeast Climate Sci Ctr, Raleigh, NC USA. [Collazo, Jaime A.] US Geol Survey, Raleigh, NC USA. RP Khalyani, AH (reprint author), US Forest Serv, USDA, Int Inst Trop Forestry, 1201 Calle Ceiba, San Juan, PR 00926 USA. EM ahenareh@mtu.edu OI Gould, William/0000-0002-3720-9735 NR 70 TC 3 Z9 3 U1 3 U2 13 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1558-8424 EI 1558-8432 J9 J APPL METEOROL CLIM JI J. Appl. Meteorol. Climatol. PD FEB PY 2016 VL 55 IS 2 BP 265 EP 282 DI 10.1175/JAMC-D-15-0182.1 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DE1WX UT WOS:000370418300001 ER PT J AU Couturier, K Hininger, I Poulet, L Anderson, RA Roussel, AM Canini, F Batandier, C AF Couturier, Karine Hininger, Isabelle Poulet, Laurent Anderson, Richard A. Roussel, Anne-Marie Canini, Frederic Batandier, Cecile TI Cinnamon intake alleviates the combined effects of dietary-induced insulin resistance and acute stress on brain mitochondria SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Polyphenols; Brain; Mitochondria; Acute stress; Insulin resistance; Permeability transition pore ID PERMEABILITY TRANSITION PORE; RESPIRATORY-CHAIN; METABOLIC SYNDROME; IN-VITRO; POLYPHENOLS; RATS; FAT; DYSFUNCTION; GLUCOSE; ROS AB Insulin resistance (IR), which is a leading cause of the metabolic syndrome, results in early brain function alterations which may alter brain mitochondrial functioning. Previously, we demonstrated that rats fed a control diet and submitted to an acute restraint stress exhibited a delayed mitochondria] permeability transition pore (mPTP) opening. In this study, we evaluated the combined effects of dietary and emotional stressors as found in western way of life. We studied, in rats submitted or not to an acute stress, the effects of diet-induced IR on brain mitochondria, using a high fat/high fructose diet (HF2), as an IR inducer, with addition or not of cinnamon as an insulin sensitizer. We measured Ca2+ retention capacity, respiration, ROS production, enzymatic activities and cell signaling activation. Under stress, HF2 diet dramatically decreased the amount of Ca2+ required to open the mPTP (13%) suggesting an adverse effect on mitochondrial survival. Cinnamon added to the diet corrected this negative effect and resulted in a partial recovery (30%). The effects related to cinnamon addition to the diet could be due to its antioxidant properties or to the observed modulation of PI3K-AKT-GSK3 beta and MAPK-P38 pathways or to a combination of both. These data suggest a protective effect of cinnamon on brain mitochondria against the negative impact of an HF2 diet. Cinnamon could be beneficial to counteract deleterious dietary effects in stressed conditions. (C) 2015 Elsevier Inc. All rights reserved. C1 [Couturier, Karine; Hininger, Isabelle; Poulet, Laurent; Roussel, Anne-Marie; Batandier, Cecile] Univ Grenoble Alpes, Lab Bioenerget Fondamentale & Appl, F-38041 Grenoble, France. [Couturier, Karine; Hininger, Isabelle; Poulet, Laurent; Roussel, Anne-Marie; Batandier, Cecile] INSERM, U1055, F-38041 Grenoble, France. [Anderson, Richard A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Canini, Frederic] Inst Rech Biomed Armees IRBA, Dept Neurosci & Contraintes Operat, BP73, F-91223 Bretigny Sur Orge, France. [Canini, Frederic] Ecole Val Grace, 1 Pl Alphonse Laveran, F-75230 Paris, France. [Anderson, Richard A.] PolyChrom Technol, Edgewater, MD USA. RP Batandier, C (reprint author), Univ Grenoble Alpes, Lab Bioenerget Fondamentale & Appl, INSERM, U1055, BP 53X, F-38041 Grenoble, France. EM Cecile.Batandier@ujf-grenoble.fr FU French National Agency for Research [PNRA 007]; ANR-Paris-France [ANR-07-PNRA-003-01] FX This work is a part of the CERVIRMIT Study funded by the French National Agency for Research (PNRA 007): ANR-Paris-France (grant number ANR-07-PNRA-003-01). NR 47 TC 1 Z9 1 U1 2 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD FEB PY 2016 VL 28 BP 183 EP 190 DI 10.1016/j.jnutbio.2015.10.016 PG 8 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DF4YS UT WOS:000371359100020 PM 26878796 ER PT J AU Lim, HJ Sew, EY Kim, HS Kim, JK Park, CH Gong, JS Kim, IH Han, SH Kilcrease, JP Furuya, N Tsuchiya, K Hammond, J Lim, HS AF Lim, Hyo-Jin Sew, Eun-Young Kim, Hyun-Seung Kim, Jung-Kyu Park, Chan-Hwan Gong, Jun-Su Kim, Ik-hyun Han, Sang-huk Kilcrease, James P. Furuya, Naruto Tsuchiya, Kenichi Hammond, John Lim, Hyoun-Sub TI 2015 Nationwide Survey Revealed Barley stripe mosaic virus in Korean Barley Fields SO JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY LA English DT Article DE Barley stripe mosaic virus; Nationwide survey; Seed transmission AB A seed transmitted virus has consistently caused significant economic damage to barley crops in Korea in recent years, and may be increasing because many farmers save seed for replanting. Because some barley seed is imported, there is the potential for introduction of new seed transmitted viruses, causing diseases which may spread. Barley cultivation in South Korea is expanding nationwide due to the increasing popularity of health foods, so both production and quality of barley grain is important. Although Barley stripe mosaic virus (BSMV) has been reported previously as a small percentage of all barley viruses present in Korea, increases in imports of barley seed may lead to increased occurrence of this seed transmitted virus. We therefore investigated the virus status of barley crops around Iksan and Wanju. On several newly cultivated barley farms, we observed areas showing symptoms typical of BSMV, and confirmed BSMV infection in 24% of the samples examined. In order to understand the occurrence and seed transmission of Korean BSMV isolates, we examined sequence variation within the Triple Gene Block proteins, and subcellular localization of two of these proteins. The newly identified Korean BSMV isolates show low sequence variability and high sequence homology to previously reported US isolates. With these results, we expect to confirm distribution of barley viruses and possible emerging viruses, which will serve as base line data to document virus prevention and control measures. C1 Kyushu Univ, Dept Bioresource Sci, Div Agr Bioresource Sci, Lab Plant Pathol,Fac Agr, Fukuoka 8128581, Japan. [Lim, Hyo-Jin; Sew, Eun-Young; Kim, Hyun-Seung; Kim, Jung-Kyu; Park, Chan-Hwan; Gong, Jun-Su; Kim, Ik-hyun; Han, Sang-huk; Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Coll Agr & Life Sci, Taejon 305764, South Korea. [Kilcrease, James P.; Hammond, John] ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. [Furuya, Naruto; Tsuchiya, Kenichi] Kyushu Univ, Dept Agr Bioresource Sci, 6-10-1 Hakozaki, Fukuoka 8128581, Japan. RP Lim, HS (reprint author), Chungnam Natl Univ, Dept Appl Biol, Coll Agr & Life Sci, Taejon 305764, South Korea.; Hammond, J (reprint author), ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA.; Furuya, N; Tsuchiya, K (reprint author), Kyushu Univ, Dept Agr Bioresource Sci, 6-10-1 Hakozaki, Fukuoka 8128581, Japan. EM kentsuch@agr.kyushu-u.ac.jp; john.hammond@usda.ars.gov; hyounlim@cnu.ac.kr FU iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs [113044-3]; Cooperative Research Program for Agriculture Science and Technology Development, Rural Development Administration, Republic of Korea [PJ01007703201401] FX This research was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs (Project No. 113044-3) and carried out with the support of Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01007703201401), Rural Development Administration, Republic of Korea. NR 16 TC 0 Z9 0 U1 1 U2 2 PU KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS PI FUKUOKA-SHI PA 6-10-1 HAKOZAKI, HIGASHI-KU, FUKUOKA-SHI, 812-0053, JAPAN SN 0023-6152 J9 J FAC AGR KYUSHU U JI J. Fac. Agric. Kyushu Univ. PD FEB PY 2016 VL 61 IS 1 BP 71 EP 77 PG 7 WC Agriculture, Multidisciplinary SC Agriculture GA DF5EW UT WOS:000371375600012 ER PT J AU Hald, T Angulo, F Bin Hamzah, WM Bellinger, D Black, R de Silva, N Dopfer, D Havelaar, A Gibb, H Kasuga, F Lake, R Rokni, MB Speybroeck, N Aspinall, W Cooke, R Hoffmann, S Devleesschauwer, B Pires, SM AF Hald, Tine Angulo, Fred Bin Hamzah, Wan Mansor Bellinger, David Black, Robert de Silva, Nilanthi Doepfer, Doerte Havelaar, Arie Gibb, Herman Kasuga, Fumiko Lake, Rob Rokni, Muhammad B. Speybroeck, Niko Aspinall, Willy Cooke, Roger Hoffmann, Sandra Devleesschauwer, Brecht Pires, Sara M. CA World Hlth Org Foodborne Epidemiology Reference G Source Attribution Task Force TI Research Synthesis Methods in an Age of Globalized Risks: Lessons from the Global Burden of Foodborne Disease Expert Elicitation SO RISK ANALYSIS LA English DT Editorial Material DE Disease burden; expert elicitation; expert judgment; exposure estimates; foodborne illness; research synthesis; source attribution; systematic review; uncertainty quantification ID ADJUSTED LIFE YEARS; PATHOGENS; JUDGMENT; MODEL; FOOD AB We live in an age that increasingly calls for national or regional management of global risks. This article discusses the contributions that expert elicitation can bring to efforts to manage global risks and identifies challenges faced in conducting expert elicitation at this scale. In doing so it draws on lessons learned from conducting an expert elicitation as part of the World Health Organizations (WHO) initiative to estimate the global burden of foodborne disease; a study commissioned by the Foodborne Disease Epidemiology Reference Group (FERG). Expert elicitation is designed to fill gaps in data and research using structured, transparent methods. Such gaps are a significant challenge for global risk modeling. Experience with the WHO FERG expert elicitation shows that it is feasible to conduct an expert elicitation at a global scale, but that challenges do arise, including: defining an informative, yet feasible geographical structure for the elicitation; defining what constitutes expertise in a global setting; structuring international, multidisciplinary expert panels; and managing demands on experts' time in the elicitation. This article was written as part of a workshop, Methods for Research Synthesis: A Cross-Disciplinary Approach held at the Harvard Center for Risk Analysis on October 13, 2013. C1 [Hald, Tine; Pires, Sara M.] Tech Univ Denmark, Natl Food Inst, Lyngby, Denmark. [Angulo, Fred] US Ctr Dis Control & Prevent, Atlanta, GA USA. [Bin Hamzah, Wan Mansor] Malaysian Minist Hlth, Dis Control Div, Kuala Lumpur, Malaysia. [Bellinger, David] Harvard Univ, Sch Publ Hlth, Cambridge, MA 02138 USA. [Black, Robert] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD 21218 USA. [de Silva, Nilanthi] Univ Kelaniya, Fac Med, Kelaniya, Sri Lanka. [Doepfer, Doerte] Univ Wisconsin Madison, Sch Vet Med, Madison, WI USA. [Havelaar, Arie; Devleesschauwer, Brecht] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Gibb, Herman] Gibb Epidemiol Consulting LLC, Arlington, VA USA. [Kasuga, Fumiko] Japanese Minist Hlth Labour & Welfare, Natl Inst Hlth Sci, Tokyo, Japan. [Lake, Rob] Inst Environm Sci & Res ESR Ltd, Tokyo, Japan. [Rokni, Muhammad B.] Teheran Univ Med Sci, Tehran, Iran. [Speybroeck, Niko] Catholic Univ Louvain, Louvain, Belgium. [Aspinall, Willy] Univ Bristol, Bristol BS8 1TH, Avon, England. [Aspinall, Willy] Aspinall & Associates, Louvain, Belgium. [Cooke, Roger] Resources Future Inc, Louvain, Belgium. [Hoffmann, Sandra] USDA, Econ Res Serv, Washington, DC USA. RP Hoffmann, S (reprint author), USDA, Econ Res Serv, Washington, DC USA. EM shoffmann@ers.usda.gov FU World Health Organization FX This study was commissioned and paid for by the World Health Organization. Copyright in the original work on which this article is based belongs to WHO. The authors have been given permission to publish this article. NR 49 TC 0 Z9 0 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD FEB PY 2016 VL 36 IS 2 BP 191 EP 202 DI 10.1111/risa.12385 PG 12 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA DF3JJ UT WOS:000371239800002 ER PT J AU Guo, YY Tian, JC Shi, WP Dong, XH Romeis, J Naranjo, SE Hellmich, RL Shelton, AM AF Guo, Yan-Yan Tian, Jun-Ce Shi, Wang-Peng Dong, Xue-Hui Romeis, Joerg Naranjo, Steven E. Hellmich, Richard L. Shelton, Anthony M. TI The interaction of two-spotted spider mites, Tetranychus urticae Koch, with Cry protein production and predation by Amblyseius andersoni (Chant) in Cry1Ac/Cry2Ab cotton and Cry1F maize SO TRANSGENIC RESEARCH LA English DT Article DE Tri-trophic exposure; Cry1Ac; Cry2Ab; Cry1F; Environmental risk assessment; Biological control ID TRANSGENIC INSECTICIDAL CROPS; PERSIMILIS ATHIAS-HENRIOT; BT-MAIZE; BIOLOGICAL-CONTROL; METAANALYSIS RESPONSE; LEPIDOPTERA-NOCTUIDAE; ACARINA-PHYTOSEIIDAE; DIGESTIVE PHYSIOLOGY; NONTARGET ARTHROPODS; CHRYSOPERLA-CARNEA AB Crops producing insecticidal crystal (Cry) proteins from the bacterium, Bacillus thuringiensis (Bt), are an important tool for managing lepidopteran pests on cotton and maize. However, the effects of these Bt crops on non-target organisms, especially natural enemies that provide biological control services, are required to be addressed in an environmental risk assessment. Amblyseius andersoni (Acari: Phytoseiidae) is a cosmopolitan predator of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae), a significant pest of cotton and maize. Tri-trophic studies were conducted to assess the potential effects of Cry1Ac/Cry2Ab cotton and Cry1F maize on life history parameters (survival rate, development time, fecundity and egg hatching rate) of A. andersoni. We confirmed that these Bt crops have no effects on the biology of T. urticae and, in turn, that there were no differences in any of the life history parameters of A. andersoni when it fed on T. urticae feeding on Cry1Ac/Cry2Ab or non-Bt cotton and Cry1F or non-Bt maize. Use of a susceptible insect assay demonstrated that T. urticae contained biologically active Cry proteins. Cry proteins concentrations declined greatly as they moved from plants to herbivores to predators and protein concentration did not appear to be related to mite density. Free-choice experiments revealed that A. andersoni had no preference for Cry1Ac/Cry2Ab cotton or Cry1F maize-reared T. urticae compared with those reared on non-Bt cotton or maize. Collectively these results provide strong evidence that these crops can complement other integrated pest management tactics including biological control. C1 [Guo, Yan-Yan; Shelton, Anthony M.] Cornell Univ, NYSAES, Dept Entomol, Geneva, NY USA. [Guo, Yan-Yan; Shi, Wang-Peng] China Agr Univ, Dept Entomol, Beijing 100094, Peoples R China. [Tian, Jun-Ce] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China. [Dong, Xue-Hui] China Agr Univ, Dept Agr Sci, Beijing 100094, Peoples R China. [Romeis, Joerg] ISS, Agroscope, Zurich, Switzerland. [Naranjo, Steven E.] ARS, Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. [Hellmich, Richard L.] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA 50011 USA. [Hellmich, Richard L.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. RP Shelton, AM (reprint author), Cornell Univ, NYSAES, Dept Entomol, Geneva, NY USA. EM ams5@cornell.edu NR 68 TC 0 Z9 0 U1 10 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD FEB PY 2016 VL 25 IS 1 BP 33 EP 44 DI 10.1007/s11248-015-9917-1 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DF2FA UT WOS:000371155500003 PM 26545599 ER PT J AU Pineda-Garcia, F Paz, H Meinzer, FC Angeles, G AF Pineda-Garcia, Fernando Paz, Horacio Meinzer, Frederick C. Angeles, Guillermo TI Exploiting water versus tolerating drought: water-use strategies of trees in a secondary successional tropical dry forest SO TREE PHYSIOLOGY LA English DT Article DE carbon capture; drought tolerance; exploitation capacity; hydraulic conductivity; resistance to embolisms; trade-off ID RELATIVE GROWTH-RATE; PLANT ECONOMICS SPECTRUM; ECOLOGICAL GROUPS; CANOPY TREES; WOOD DENSITY; LIFE-HISTORY; RAINY-SEASON; TRADE-OFFS; LEAF; TRAITS AB In seasonal plant communities where water availability changes dramatically both between and within seasons, understanding the mechanisms that enable plants to exploit water pulses and to survive drought periods is crucial. By measuring rates of physiological processes, we examined the trade-off between water exploitation and drought tolerance among seedlings of trees of a tropical dry forest, and identified biophysical traits most closely associated with plant water-use strategies. We also explored whether early and late secondary successional species occupy different portions of trade-off axes. As predicted, species that maintained carbon capture, hydraulic function and leaf area at higher plant water deficits during drought had low photosynthetic rates, xylem hydraulic conductivity and growth rate under non-limiting water supply. Drought tolerance was associated with more dense leaf, stem and root tissues, whereas rapid resource acquisition was associated with greater stem water storage, larger vessel diameter and larger leaf area per mass invested. We offer evidence that the water exploitation versus drought tolerance trade-off drives species differentiation in the ability of tropical dry forest trees to deal with alternating water-drought pulses. However, we detected no evidence of strong functional differentiation between early and late successional species along the proposed trade-off axes, suggesting that the environmental gradient of water availability across secondary successional habitats in the dry tropics does not filter out physiological strategies of water use among species, at least at the seedling stage. C1 [Pineda-Garcia, Fernando] Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Morelia, Antigua Carretera Patzcuaro 8701, Morelia 58190, Michoacan, Mexico. [Paz, Horacio] Univ Nacl Autonoma Mexico, Inst Invest Ecosistemas & Sustentabilidad, Campus Morelia, Morelia 58190, Michoacan, Mexico. [Meinzer, Frederick C.] US Forest Serv, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Angeles, Guillermo] Inst Ecol AC, Red Ecol Func, Xalapa 91070, Veracruz, Mexico. RP Pineda-Garcia, F (reprint author), Univ Nacl Autonoma Mexico, Escuela Nacl Estudios Super, Unidad Morelia, Antigua Carretera Patzcuaro 8701, Morelia 58190, Michoacan, Mexico. EM f.pineda.garcia@gmail.com FU DGAPA-PAPIIT, UNAM [IN208012]; CONACYT [2009129740]; DGAPA-UNAM postdoctoral scholarship at the Escuela Nacional de Estudios Superiores, UNAM, Unidad Morelia FX This work was funded by the grant IN208012, DGAPA-PAPIIT, UNAM, provided to H.P., and by CONACYT 2009129740. F.P.-G. acknowledges the DGAPA-UNAM postdoctoral scholarship at the Escuela Nacional de Estudios Superiores, UNAM, Unidad Morelia. NR 61 TC 2 Z9 2 U1 10 U2 28 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD FEB PY 2016 VL 36 IS 2 BP 208 EP 217 DI 10.1093/treephys/tpv124 PG 10 WC Forestry SC Forestry GA DF2OU UT WOS:000371184000007 PM 26687176 ER PT J AU Halterman, D Guenthner, J Collinge, S Butler, N Douches, D AF Halterman, Dennis Guenthner, Joe Collinge, Susan Butler, Nathaniel Douches, David TI Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Review DE Potato; Genetic modification; Stress resistance traits; Tuber quality traits ID LATE BLIGHT RESISTANCE; GENETICALLY-MODIFIED FOODS; ZINC-FINGER NUCLEASES; SOLANUM-TUBEROSUM L.; RECESSIVE BYMOVIRUS RESISTANCE; PLANT-PARASITIC NEMATODES; BROAD-SPECTRUM RESISTANCE; INITIATION-FACTOR EIF4E; CANDIDATE GENE APPROACH; DOUBLE-STRANDED-RNA AB Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato is an intensively managed crop, requiring irrigation, fertilization, and frequent pesticide applications in order to obtain the highest yields possible. Important traits are easy to find in wild relatives of potato, but their introduction using traditional breeding can take 15-20 years. This is due to sexual incompatibility between some wild and cultivated species, a desire to remove undesirable wild species traits from adapted germplasm, and difficulty in identifying broadly applicable molecular markers. Fortunately, potato is amenable to propagation via tissue culture and it is relatively easy to introduce new traits using currently available biotech transformation techniques. For these reasons, potato is arguably the crop that can benefit most by modern biotechnology. The benefits of biotech potato, such as limited gene flow to conventionally grown crops and weedy relatives, the opportunity for significant productivity and nutritional quality gains, and reductions in production cost and environmental impact, have the potential to influence the marketability of newly developed varieties. In this review we will discuss current and past efforts to develop biotech potato varieties, traits that could be impacted, and the potential effects that biotech potato could have on the industry. C1 [Halterman, Dennis] ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. [Guenthner, Joe] Univ Idaho, Dept AERS, Moscow, ID 83844 USA. [Collinge, Susan] JR Simplot Co, Plant Sci, Boise, ID 83706 USA. [Butler, Nathaniel; Douches, David] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Halterman, D (reprint author), ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. EM dennis.halterman@ars.usda.gov FU USDA Biotechnology Risk Assessment [2013-33522-21090] FX Salary funding for N. Butler was provided by USDA Biotechnology Risk Assessment Grant number 2013-33522-21090. NR 164 TC 1 Z9 3 U1 11 U2 42 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2016 VL 93 IS 1 BP 1 EP 20 DI 10.1007/s12230-015-9485-1 PG 20 WC Agronomy SC Agriculture GA DE7UA UT WOS:000370841000001 ER PT J AU Suttle, JC Olson, LL Lulai, EC AF Suttle, Jeffrey C. Olson, Linda L. Lulai, Edward C. TI The Involvement of Gibberellins in 1,8-Cineole-Mediated Inhibition of Sprout Growth in Russet Burbank Tubers SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Mono-terpene; Potato; Solanum tuberosum; Sprouting ID SOLANUM-TUBEROSUM; ENDOGENOUS GIBBERELLINS; VOLATILE MONOTERPENES; POTATO MICROTUBERS; METABOLISM; PROHEXADIONE; MECHANISMS; EXPRESSION; HERBICIDES; TOXICITY AB The involvement of gibberellins in 1,8-cineole-mediated inhibition of tuber sprout growth was investigated in non-dormant field- and greenhouse-grown tubers of Russet Burbank. Continuous exposure of tubers to cineole in the vapor-phase resulted in a dose-dependent inhibition of sprout growth. Comparative studies using plant bioassay systems whose growth was differentially dependent on cell division, cell elongation, or both demonstrated that cineole had no direct effect on either process. Of the assays used, only cineole-mediated inhibition of etiolated hypocotyl growth mirrored the inhibition of tuber sprout growth which suggested an effect on gibberellin synthesis or action. Both GA(19) and GA(20) were detected in extracts prepared from control sprouts but only GA(19) was found in extracts prepared from cineole treated sprouts. Exogenous GA(3,) GA(20), and GA(1) (but not GA(19)) reversed cineole-mediated sprout growth inhibition. Expression of genes encoding key GA metabolic enzymes was altered by cineole treatment in a manner consistent with diminished endogenous GA content. Collectively, these results suggest that the inhibition of sprout growth by low vapor-phase concentrations of cineole is in part a result of impaired GA biosynthesis resulting in a reduction in bioactive GA content. C1 [Suttle, Jeffrey C.; Olson, Linda L.; Lulai, Edward C.] USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. RP Suttle, JC (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM jeff.suttle@ars.usda.gov NR 32 TC 0 Z9 0 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2016 VL 93 IS 1 BP 72 EP 79 DI 10.1007/s12230-015-9490-4 PG 8 WC Agronomy SC Agriculture GA DE7UA UT WOS:000370841000007 ER PT J AU Haynes, KG Qu, XS AF Haynes, Kathleen G. Qu, Xinshun TI Late Blight and Early Blight Resistance from Solanum hougasii Introgressed Into Solanum tuberosum SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato; Phytophthora infestans; Alternaria solani; Germplasm enhancement; Specific gravity; Chips processing ID POTATO LATE BLIGHT; PHYTOPHTHORA-INFESTANS; CHROMOSOME-IX; GENE; BULBOCASTANUM; POPULATION; GENOTYPE; TOMATO; MEXICO; LOCUS AB Late blight and early blight, caused by Phytophthora infestans and Alternaria solani, respectively, are the two most widely occurring foliar diseases of potato in the U.S.A. Resistance to both diseases is necessary if growers are to reduce fungicide applications. Field resistance to late blight has previously been reported in an accession of Solanum hougasii (2n = 72). The putative aneuploid clone E53.61, derived from (S. hougasii x S. tuberosum) x S. tuberosum was obtained from C.R. Brown and crossed with three S. tuberosum clones. Thirty-five hybrid clones were evaluated for foliar late blight resistance at the Russell E. Larson Agricultural Research Center near State College, PA along with the susceptible check 'Atlantic' and for foliar early blight resistance in Presque Isle, ME along with the susceptible check 'Harley Blackwell' for 3 years (2012 to 2014). The experimental design was a randomized complete block design with two to three replications each year. The US-23 genotype of P. infestans occurred naturally and/or was used in inoculations in PA and plants were infected naturally with A. solani in ME. Relative area under the disease progress curve (RAUDPC) values were calculated based on visual assessment of foliar disease four to five times late in the season each year and subjected to statistical and stability analyses. There were significant differences among clones and the clone x environment interaction was significant for both diseases. Of the 35 hybrid clones evaluated for late blight, 16 were more resistant, 7 were more susceptible, and 12 were as susceptible as 'Atlantic'. Of those same hybrid clones evaluated for early blight, 23 were more resistant than 'Harley Blackwell'; the rest were as susceptible. Late blight resistance or susceptibility was independent of the stability of resistance, however, early blight resistance was associated with greater stability. Fourteen clones were more resistant than the check varieties for both late blight and early blight, suggesting that resistance genes for both late blight and early blight have been combined in this genetic material; three of these clones also had high specific gravity and acceptable chip color out of 10 A degrees C storage. C1 [Haynes, Kathleen G.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Qu, Xinshun] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Haynes, KG (reprint author), USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. EM kathleen.haynes@ars.usda.gov NR 38 TC 0 Z9 0 U1 10 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2016 VL 93 IS 1 BP 86 EP 95 DI 10.1007/s12230-015-9492-2 PG 10 WC Agronomy SC Agriculture GA DE7UA UT WOS:000370841000009 ER PT J AU Cheng, Y Engling, G Moosmaller, H Arnott, WP Chen, LWA Wold, CE Hao, WM He, KB AF Cheng, Yuan Engling, Guenter Moosmaller, Hans Arnott, W. Patrick Chen, L. -W. Antony Wold, Cyle E. Hao, Wei Min He, Ke-bin TI Light absorption by biomass burning source emissions SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Biomass burning; Light absorption; Black carbon; Photoacoustic; Liquid-like organics ID DIFFERENT TEMPERATURE PROTOCOLS; AEROSOL OPTICAL-PROPERTIES; FILTER-BASED MEASUREMENTS; BLACK CARBON AEROSOL; ELEMENTAL CARBON; ORGANIC AEROSOL; BROWN CARBON; MASS-SPECTROMETRY; MIXING STATE; PARTICLES AB Black carbon (BC) aerosol has relatively short atmospheric lifetimes yet plays a unique and important role in the Earth's climate system, making it an important short-term climate mitigation target. Globally, biomass burning is the largest source of BC emissions into the atmosphere. This study investigated the mass absorption efficiency (MAE) of biomass burning BC generated by controlled combustion of various wildland fuels during the Fire Laboratory at Missoula Experiments (FLAME). MAE values derived from a photoacoustic spectrometer (similar to 7.8 m(2)/g at a wavelength of 532 nm) were in good agreement with those suggested for uncoated BC when the emission ratios of organic carbon (OC) to elemental carbon (EC) were extremely low (i.e., below 0.3). With the increase of OC/EC, two distinct types of biomass smoke were identified. For the first type, MAE exhibited a positive dependence on OC/EC, while the overestimation of the light absorption coefficient (babs) by a filter-based method was less significant and could be estimated by a nearly constant correction factor. For the second type, MAE was biased low and correlated negatively with OC/EC, while the overestimation of babs by the filter-based method was much more significant and showed an apparent OC/EC dependence. This study suggests that BC emission factors determined by the commonly used thermal-optical methods might be sustantially overestimated for some types of biomass burning emissions. Our results also indicate that biomass burning emissions may include some liquid-like organics that can significantly bias filter-based babs measurements. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Cheng, Yuan; He, Ke-bin] Tsinghua Univ, Sch Environm, State Key joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China. [Engling, Guenter; Moosmaller, Hans; Arnott, W. Patrick; Chen, L. -W. Antony] Univ Nevada, Desert Res Inst, Div Atmospher Sci, Reno, NV 89506 USA. [Arnott, W. Patrick] Univ Nevada, Dept Phys, Reno, NV 89557 USA. [Chen, L. -W. Antony] Univ Nevada, Dept Environm & Occupat Hlth, Las Vegas, NV 89154 USA. [Wold, Cyle E.; Hao, Wei Min] US Forest Serv, Fire Sci Lab, USDA, Missoula, MT USA. [He, Ke-bin] State Environm Protect Key Lab Sources & Control, Beijing, Peoples R China. [He, Ke-bin] Collaborat Innovat Ctr Reg Environm Qual, Beijing, Peoples R China. RP Engling, G (reprint author), Univ Nevada, Desert Res Inst, Div Atmospher Sci, Reno, NV 89506 USA. EM ycheng@mail.tsinghua.edu.cn; Guenter.Engling@dri.edu RI Cheng, Yuan/E-2508-2011; Chen, Lung-Wen/J-5792-2015; OI Cheng, Yuan/0000-0002-2077-5335; Chen, Lung-Wen/0000-0002-2311-7506; Moosmuller, Hans/0000-0002-1021-8877 FU National Natural Science Foundation of China [21307067]; Tsinghua University [20131089241]; International Postdoctoral Exchange Fellowship Program; USFS, Rocky Mountain Research Station, Research Work [4404, 03-JV-11222049-102]; DRI [03-JV-11222049-102]; Joint Fire Science Project through NPS Task [J8R07060005]; NASA EPSCoR [NNX10AR89A]; NASA ROSES [NNX11AB79G] FX The first author was supported by the National Natural Science Foundation of China under Grant No. 21307067, by Tsinghua University under Grant No. 20131089241, and by the International Postdoctoral Exchange Fellowship Program. This work was also supported by the Joint Venture Agreement 03-JV-11222049-102 between the USFS, Rocky Mountain Research Station, Research Work Unit Number 4404 and DRI, by the Joint Fire Science Project through NPS Task J8R07060005, by NASA EPSCoR under Cooperative Agreement No. NNX10AR89A, and by NASA ROSES under Grant No. NNX11AB79G. NR 58 TC 1 Z9 1 U1 15 U2 40 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD FEB PY 2016 VL 127 BP 347 EP 354 DI 10.1016/j.atmosenv.2015.12.045 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DE6VC UT WOS:000370770700038 ER PT J AU Cordeiro, AM Figueiredo, DD Tepperman, J Borba, AR Lourenco, T Abreu, IA Ouwerkerk, PBF Quail, PH Oliveira, MM Saibo, NJM AF Cordeiro, Andre M. Figueiredo, Duarte D. Tepperman, James Borba, Ana Rita Lourenco, Tiago Abreu, Isabel A. Ouwerkerk, Pieter B. F. Quail, Peter H. Oliveira, M. Margarida Saibo, Nelson J. M. TI Rice phytochrome-interacting factor protein OsPIF14 represses OsDREB1B gene expression through an extended N-box and interacts preferentially with the active form of phytochrome B SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS LA English DT Article DE Rice phytochrome-interacting factor 14 (OsPIF14); OsDREB1B; Protein-DNA interaction; Alternative splicing; Light regulation; Cold stress ID LOOP-HELIX PROTEIN; ORYZA-SATIVA L.; TRANSCRIPTION FACTORS; LOW-TEMPERATURE; ARABIDOPSIS-THALIANA; SIGNAL-TRANSDUCTION; RED-LIGHT; MEDIATED DEGRADATION; FUNCTIONAL-ANALYSIS; STRESS RESPONSES AB DREB1/CBF genes, known as major regulators of plant stress responses, are rapidly and transiently induced by low temperatures. Using a yeast one-hybrid screening, we identified a putative Phytochrome-Interacting bHLH Factor (OsPIF14), as binding to the OsDREB1B promoter. bHLH proteins are able to bind to hexameric E-box (CANNTG) or N-box (CACG(A/C)G) motifs, depending on transcriptional activity. We have shown that OsPIF14 binds to the OsDREB1B promoter through two N-boxes and that the flanking regions of the hexameric core are essential for protein-DNA interaction and stability. We also showed that OsPIF14 down-regulates OsDREB1B gene expression in rice protoplasts, corroborating the OsPIF14 repressor activity observed in the transactivation assays using Arabidopsis protoplasts. In addition, we showed that OsPIF14 is indeed a phytochrome interacting factor, which preferentially binds to the active form (Pfr) of rice phytochrome B. This raises the possibility that OsPIF14 activity might be modulated by light. However, we did not observe any regulation of the OsDREB1B gene expression by light under control conditions. Moreover, OsPIF14 gene expression was shown to be modulated by different treatments, such as drought, salt, cold and ABA. Interestingly, OsPIF14 showed also a specific cold-induced alternative splicing. All together, these results suggest the possibility that OsPIF14 is involved in cross-talk between light and stress signaling through interaction with the OsDREB1B promoter. Although in the absence of stress, OsDREB1B gene expression was not regulated by light, given previous reports, it remains possible that OsPIF14 has a role in light modulation of stress responses. (C) 2015 Published by Elsevier B.V. C1 [Cordeiro, Andre M.; Figueiredo, Duarte D.; Borba, Ana Rita; Lourenco, Tiago; Abreu, Isabel A.; Oliveira, M. Margarida; Saibo, Nelson J. M.] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Genom Plant Stress Unit, Ave Republ, P-2780157 Oeiras, Portugal. [Cordeiro, Andre M.; Figueiredo, Duarte D.; Borba, Ana Rita; Lourenco, Tiago; Abreu, Isabel A.; Oliveira, M. Margarida; Saibo, Nelson J. M.] Inst Biol Expt & Tecnol, Ave Republ, P-2780157 Oeiras, Portugal. [Tepperman, James; Quail, Peter H.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Tepperman, James; Quail, Peter H.] Univ Calif Berkeley, USDA, PGEC, Albany, CA 94710 USA. [Ouwerkerk, Pieter B. F.] Leiden Univ, Inst Biol, Leiden, Netherlands. [Figueiredo, Duarte D.] Uppsala BioCtr, Dept Plant Biol, Uppsala, Sweden. [Figueiredo, Duarte D.] Univ Agr Sci Uppsala, Uppsala, Sweden. [Figueiredo, Duarte D.] Linnean Ctr Plant Biol, Uppsala, Sweden. RP Saibo, NJM (reprint author), Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Genom Plant Stress Unit, Ave Republ, P-2780157 Oeiras, Portugal.; Saibo, NJM (reprint author), Inst Biol Expt & Tecnol, Ave Republ, P-2780157 Oeiras, Portugal. EM saibo@itqb.unl.pt RI Oliveira, M. Margarida/H-9318-2012; Lourenco, Tiago/A-7175-2011; Abreu, Isabel/I-5081-2013; Cordeiro, Andre/B-8310-2016; OI Oliveira, M. Margarida/0000-0002-0095-1952; Lourenco, Tiago/0000-0001-5085-861X; Abreu, Isabel/0000-0002-5566-2146; Cordeiro, Andre/0000-0002-7299-8944; Saibo, Nelson/0000-0002-6679-3811; Figueiredo, Duarte/0000-0001-7990-3592 FU Fundacao para a Ciencia e Tecnologia (FCC) [SFRH/BD/74946/2010, SFRH/BD/29258/2006, SFRH/BPD/102872/2014]; POPH (QREN) [SFRH/BPD/78314/2011]; National Institutes of Health [GM-R01-47475]; Department of Energy [DE-FG03-87ER13742]; Agricultural Research Service Current Research Information System [5335-21000-032-00D]; Research unit GREEN-it "Bioresources for Sustainability" [UID/Multi/04551/2013]; FCT [POCI/BIA-BCM/56063/2004, PTDC/BIA_BCM/099836/2008] FX Funding: A.M.C., D.D.F. and T.L. were financed by Fundacao para a Ciencia e Tecnologia (FCC) through the fellowship SFRH/BD/74946/2010, SFRH/BD/29258/2006 and SFRH/BPD/102872/2014, respectively. I.A.A. was supported by SFRH/BPD/78314/2011 and FCC Investigator, financed by POPH (QREN). N.S. was supported by Programa Ciencia 2007 and FCT Investigator, financed by POPH (QREN). This work was funded by National Institutes of Health Grant GM-R01-47475, Department of Energy Grant DE-FG03-87ER13742, and USDA Agricultural Research Service Current Research Information System Grant 5335-21000-032-00D to P.H.Q, by Research unit GREEN-it "Bioresources for Sustainability" (UID/Multi/04551/2013) and by FCT projects POCI/BIA-BCM/56063/2004 and PTDC/BIA_BCM/099836/2008. NR 61 TC 7 Z9 7 U1 8 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-9399 EI 0006-3002 J9 BBA-GENE REGUL MECH JI Biochim. Biophys. Acta-Gene Regul. Mech. PD FEB PY 2016 VL 1859 IS 2 BP 393 EP 404 DI 10.1016/j.bbagrm.2015.12.008 PG 12 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DE8PK UT WOS:000370898500016 PM 26732823 ER PT J AU Hagstrom, AD Marshall, PWM Lonsdale, C Papalia, S Cheema, BS Toben, C Baune, BT Singh, MAF Green, S AF Hagstrom, Amanda D. Marshall, Paul W. M. Lonsdale, Chris Papalia, Shona Cheema, Birinder S. Toben, Catherine Baune, Bernhard T. Singh, Maria A. Fiatarone Green, Simon TI The effect of resistance training on markers of immune function and inflammation in previously sedentary women recovering from breast cancer: a randomized controlled trial SO BREAST CANCER RESEARCH AND TREATMENT LA English DT Article DE Resistance training; Immune function; Inflammation; Breast cancer survivors ID TUMOR-NECROSIS-FACTOR; NATURAL-KILLER-CELLS; PERIPHERAL-BLOOD LYMPHOCYTES; QUALITY-OF-LIFE; RECEIVING ADJUVANT CHEMOTHERAPY; BONE-MINERAL DENSITY; C-REACTIVE PROTEIN; FACTOR-ALPHA; TNF-ALPHA; DIFFERENTIAL EXPRESSION AB The purpose of this randomized controlled trial was to determine the effects of resistance training (RT) on markers of inflammation and immune function in breast cancer survivors. Thirty-nine breast cancer survivors were randomly assigned to a RT (n = 20) or control (n = 19) group. RT performed supervized exercise three times per week. Natural killer cell (NK) and natural killer T-cell (NKT) function, and markers of inflammation (serum TNF-alpha, IL-6, IL-10, and CRP) were measured before and after training. Changes in NK and NKT cell function were analyzed using ANCOVA, with the change score the dependent variable, and the baseline value of the same variable the covariate. Effect sizes (ES) were calculated via partial eta-squared. We found a significant reduction, and large associated ESs, in the RT group compared to the control group for change in NK cell expression of TNF-alpha (p = 0.005, ES = 0.21) and NKT cell expression of TNF-alpha (p = 0.04, ES = 0.12). No differences were observed in any serum marker. Significant improvements in all measurements of strength were found in RT compared to control (p < 0.001; large ESs ranging from 0.32 to 0.51). These data demonstrate that RT has a beneficial effect on the NK and NKT cell expression of TNF-alpha indicating that RT may be beneficial in improving the inflammatory profile in breast cancer survivors. C1 [Hagstrom, Amanda D.] Univ New England, Sch Sci & Technol, Armidale, NSW, Australia. [Hagstrom, Amanda D.; Marshall, Paul W. M.; Papalia, Shona; Cheema, Birinder S.; Green, Simon] Univ Western Sydney, Sch Sci & Hlth, Campbelltown, NSW, Australia. [Lonsdale, Chris] Australian Catholic Univ, Inst Posit Psychol & Educ, Strathfield, NSW, Australia. [Cheema, Birinder S.] Univ Western Sydney, NICM, Campbelltown, NSW, Australia. [Toben, Catherine; Baune, Bernhard T.] Univ Adelaide, Sch Med, Adelaide, SA, Australia. [Singh, Maria A. Fiatarone] Univ Sydney, Exercise Hlth & Performance Res Grp, Sydney, NSW 2006, Australia. [Singh, Maria A. Fiatarone] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. [Singh, Maria A. Fiatarone] Tufts Univ, Hebrew Senior Life & Jean Mayer USDA Human Nutr C, Boston, MA 02111 USA. [Green, Simon] Univ Western Sydney, Sch Med, Campbelltown, NSW, Australia. RP Hagstrom, AD (reprint author), Univ New England, Sch Sci & Technol, Armidale, NSW, Australia.; Hagstrom, AD (reprint author), Univ Western Sydney, Sch Sci & Hlth, Campbelltown, NSW, Australia. EM ahagstro@une.edu.au; p.marshall@westernsydney.edu.au; chris.lonsdale@acu.edu.au; s.papalia@westernsydney.edu.au; b.cheema@westernsydney.edu.au; catherine.toben@adelaide.edu.au; bernhard.baune@adelaide.edu.au; maria.fiataronesingh@sydney.edu.au; simon.green@westernsydney.edu.au OI Lonsdale, Chris/0000-0002-2523-5565 FU Western Sydney University, Australia FX This study was supported by a grant from Western Sydney University, Australia. NR 86 TC 2 Z9 2 U1 2 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0167-6806 EI 1573-7217 J9 BREAST CANCER RES TR JI Breast Cancer Res. Treat. PD FEB PY 2016 VL 155 IS 3 BP 471 EP 482 DI 10.1007/s10549-016-3688-0 PG 12 WC Oncology SC Oncology GA DF0UR UT WOS:000371055100008 PM 26820653 ER PT J AU Welch, KD Whitney, TD Harwood, JD AF Welch, K. D. Whitney, T. D. Harwood, J. D. TI Non-pest prey do not disrupt aphid predation by a web-building spider SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE prey choice; alternative prey; optimal foraging; olfactory cues; spider webs; Araneae; Linyphiidae; Collembola ID MIXED-SPECIES DIETS; ALTERNATIVE PREY; LINYPHIID SPIDERS; GENERALIST PREDATORS; WINTER-WHEAT; RHOPALOSIPHUM-PADI; JUMPING SPIDER; FOOD; COLLEMBOLA; ARANEAE AB A generalist predator's ability to contribute to biological control is influenced by the decisions it makes during foraging. Predators often use flexible foraging tactics, which allows them to pursue specific types of prey at the cost of reducing the likelihood of capturing other types of prey. When a pest insect has low nutritional quality or palatability for a predator, the predator is likely to reject that prey in favour of pursuing alternative, non-pest prey. This is often thought to limit the effectiveness of generalist predators in consuming aphids, which are of low nutritional quality for many generalist predators. Here, we report behavioural assays that test the hypothesis that the generalist predator, Grammonota inornata (Araneae: Linyphiidae), preferentially forages for a non-pest prey with high nutritional quality (springtails), and rejects a pest prey with low nutritional quality (aphids). In no-choice assays, molecular gutcontent analysis revealed that spiders continued to feed on the low-quality aphids at high rates, even when high-quality springtails were readily available. When provided a choice between aphids and springtails in two-way choice tests, spiders did not show the expected preference for springtails. Decision-making by spiders during foraging therefore appears to be sub-optimal, possibly because of attraction to the less frequently encountered of two preys as part of a dietary diversification strategy. These results indicate that behavioural preferences alone do not necessarily compromise the pest-suppression capacity of natural enemies: even nutritionally sub-optimal pest prey can potentially be subject to predation and suppression by natural enemies. C1 [Welch, K. D.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Welch, K. D.; Whitney, T. D.; Harwood, J. D.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. [Welch, K. D.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Whitney, T. D.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Welch, KD (reprint author), USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA.; Welch, KD (reprint author), Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.; Welch, KD (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM keltondouglaswelch@gmail.com FU Kentucky Agricultural Experiment Station [15-08-007]; National Institute of Food and Agriculture, U.S. Department of Agriculture, McIntire-Stennis project [1007196]; National Science Foundation FX This is publication No. 15-08-007 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This work is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, McIntire-Stennis project under Accession Number 1007196. Support for this research was also provided by the National Science Foundation Graduate Research Fellowship Programme. Founding stock for springtails colonies were donated by Dr Ann Rypstra (Miami University Ohio), and ultimately derived from the 'Crossley culture' maintained by Dr Michael L. Draney (University of Wisconsin-Green Bay). Katelyn Kowles (University of Kentucky) provided founding stock for aphid colonies. Mention of product information does not constitute endorsement by the USDA. NR 38 TC 0 Z9 0 U1 3 U2 16 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD FEB PY 2016 VL 106 IS 1 BP 91 EP 98 DI 10.1017/S0007485315000875 PG 8 WC Entomology SC Entomology GA DF2FJ UT WOS:000371157000010 PM 26584533 ER PT J AU Lewis, MW Hake, S AF Lewis, Michael W. Hake, Sarah TI Keep on growing: building and patterning leaves in the grasses SO CURRENT OPINION IN PLANT BIOLOGY LA English DT Review ID MAIZE ZEA-MAYS; INFLORESCENCE ARCHITECTURE; REGULATORY NETWORK; LEAF DEVELOPMENT; HOMEOBOX GENES; LATERAL DOMAIN; MESSENGER-RNA; PROTEIN; RICE; ARABIDOPSIS AB Monocot leaves have unique features that arise early in their development. Maturing leaves protectively enclose younger leaves and the meristem, the pool of founder cells from which a leaf emerges. Through the maturation process, proximal sheath and distal blade tissues differentiate and are separated by the ligule and auricle structures. Here we review current research focusing on the contribution of gene regulatory factors and phytohormones on the patterning and differentiation of monocot leaves primarily focusing on research in the grasses (Poaceae). The 10 000 members of the grasses include the true grain cereals (wheat, rice, maize, etc.), biofuel crops such as sugarcane, pasture grasses, and bamboo. They are the most studied of the monocots due to their tremendous agricultural and agronomic importance. C1 [Lewis, Michael W.] USDA ARS, Ctr Plant Gene Express, Berkeley, CA USA. Univ Calif Berkeley, Berkeley, CA 94720 USA. RP Lewis, MW (reprint author), USDA ARS, Ctr Plant Gene Express, Berkeley, CA USA. EM mluudensis@gmail.com FU United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) Postdoctoral Fellowship [2011-67012-30740]; National Science Foundation (NSF) grant [DEB-1456782] FX We thank Hake lab members for reading and discussions on this work. This work was funded by a United States Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) Postdoctoral Fellowship to M.W.L. [2011-67012-30740] and National Science Foundation (NSF) grant [DEB-1456782] to S.H. NR 44 TC 1 Z9 1 U1 7 U2 25 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1369-5266 EI 1879-0356 J9 CURR OPIN PLANT BIOL JI Curr. Opin. Plant Biol. PD FEB PY 2016 VL 29 BP 80 EP 86 PG 7 WC Plant Sciences SC Plant Sciences GA DE8OG UT WOS:000370895100013 PM 26751036 ER PT J AU Lozier, JD Jackson, JM Dillon, ME Strange, JP AF Lozier, Jeffrey D. Jackson, Jason M. Dillon, Michael E. Strange, James P. TI Population genomics of divergence among extreme and intermediate color forms in a polymorphic insect SO ECOLOGY AND EVOLUTION LA English DT Article DE Bumble bees; color pattern; divergence; gene flow; population genomics; RAD-tags; RNAseq ID SINGLE NUCLEOTIDE POLYMORPHISMS; AMERICAN BUMBLE BEES; REPRODUCTIVE ISOLATION; GENE FLOW; HELICONIUS-BUTTERFLIES; HYMENOPTERA APIDAE; HYBRID ZONE; BOMBUS-MELANOPYGUS; ADAPTIVE RADIATION; SHIFTING BALANCE AB Geographic variation in insect coloration is among the most intriguing examples of rapid phenotypic evolution and provides opportunities to study mechanisms of phenotypic change and diversification in closely related lineages. The bumble bee Bombus bifarius comprises two geographically disparate color groups characterized by red-banded and black-banded abdominal pigmentation, but with a range of spatially and phenotypically intermediate populations across western North America. Microsatellite analyses have revealed that B.bifarius in the USA are structured into two major groups concordant with geography and color pattern, but also suggest ongoing gene flow among regional populations. In this study, we better resolve the relationships among major color groups to better understand evolutionary mechanisms promoting and maintaining such polymorphism. We analyze >90,000 and >25,000 single-nucleotide polymorphisms derived from transcriptome (RNAseq) and double digest restriction site associated DNA sequencing (ddRAD), respectively, in representative samples from spatial and color pattern extremes in B.bifarius as well as phenotypic and geographic intermediates. Both ddRAD and RNAseq data illustrate substantial genome-wide differentiation of the red-banded (eastern) color form from both black-banded (western) and intermediate (central) phenotypes and negligible differentiation among the latter populations, with no obvious admixture among bees from the two major lineages. Results thus indicate much stronger background differentiation among B.bifarius lineages than expected, highlighting potential challenges for revealing loci underlying color polymorphism from population genetic data alone. These findings will have significance for resolving taxonomic confusion in this species and in future efforts to investigate color-pattern evolution in B.bifarius and other polymorphic bumble bee species. C1 [Lozier, Jeffrey D.; Jackson, Jason M.] Univ Alabama, Dept Biol Sci, Box 870344, Tuscaloosa, AL 35401 USA. [Dillon, Michael E.] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Dillon, Michael E.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Strange, James P.] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. RP Lozier, JD (reprint author), Univ Alabama, Dept Biol Sci, Box 870344, Tuscaloosa, AL 35401 USA. EM jlozier@as.ua.edu FU U Alabama College of Arts and Sciences; National Science Foundation [DEB-1457645] FX We thank the U Alabama College of Arts and Sciences and the National Science Foundation (DEB-1457645 to JDL, MED, and JPS) for funding. NR 94 TC 3 Z9 3 U1 15 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD FEB PY 2016 VL 6 IS 4 BP 1075 EP 1091 DI 10.1002/ece3.1928 PG 17 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DF1AC UT WOS:000371069800018 PM 26811748 ER PT J AU Welch, TJ LaPatra, S AF Welch, Timothy J. LaPatra, Scott TI Yersinia ruckeri lipopolysaccharide is necessary and sufficient for eliciting a protective immune response in rainbow trout (Oncorhynchus mykiss, Walbaum) SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Yersinia ruckeri; Bacterin vaccine; LPS; Immersion vaccination ID GENE-EXPRESSION; VIBRIO-ANGUILLARUM; O-ANTIGEN; BIOTYPE 2; MACROPHAGES; BACTERINS; EFFICACY; VACCINATION; CHALLENGE; IMMERSION AB Enteric redmouth disease (ERM), caused by Yersinia ruckeri, has been controlled successfully using immersion-applied bacterin vaccines for several decades. While the host response to vaccination and the mechanism of protection of this vaccine have been elucidated, the bacterial components eliciting protection have remained unclear. Here we show that highly purified serotype O1 Y. ruckeri lipopolysaccharide (LPS) is sufficient to induce a protective response to experimental challenge in rainbow trout (Oncorhynchus mykiss). Dose response experiments demonstrated that Y. ruckeri LPS at doses of 1 ng/fish and above resulted in essentially complete protection and doses as low as 0.01 ng/fish (1.38 ng/kg) resulted in significant protection, thus demonstrating the extremely high potency of this immunogen. Analysis of the Y. ruckeri genome identified a cluster of putative O-antigen biosynthetic genes specific to serotype O1 strains. This cluster primarily consisted of genes encoding proteins predicted to function in the biosynthesis of legionamic acid, a nonulosonic acid known to be part of the O-polysaccharide repeat of O1 Y. ruckeri. Mutation of the nab2 gene, a nonulosonic acid biosynthesis gene (nab gene), resulted in production of severely truncated forms of LPS. Vaccination with bacterin vaccines derived from the nab2 mutant and its wild type parent strain demonstrated that LPS is a required component of the whole-cell bacterin vaccine and suggests that LPS is the only cellular component contributing to the protective response elicited by this vaccine. We speculate that the exceptionally high potency of Y. ruckeri LPS accounts for the unusual success of this vaccine when delivered by immersion. Published by Elsevier Ltd. C1 [Welch, Timothy J.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. [LaPatra, Scott] Clear Springs Foods Inc, Div Res, POB 712, Buhl, ID 83316 USA. RP Welch, TJ (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM tim.welch@ars.usda.gov FU USDA/Agricultural Research Service CRIS Project [1930-32000-005-00] FX The authors thank Jennifer Lipscomb (USDA ARS) and Bill Shewmaker and Robin Burkhart (Clear Springs) for their expert technical assistance and Lisa Welch and Ben LaFrentz for critical comments on the manuscript. This work is supported by the USDA/Agricultural Research Service CRIS Project 1930-32000-005-00. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S Department of Agriculture. USDA is an equal opportunity employer. The authors declare no conflicts of interest. NR 34 TC 3 Z9 3 U1 0 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD FEB PY 2016 VL 49 BP 420 EP 426 DI 10.1016/j.fsi.2015.12.037 PG 7 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DE8KF UT WOS:000370884200045 PM 26766181 ER PT J AU Chu, QQ Liu, JG Bali, K Thorp, KR Smith, R Wang, GY AF Chu, Qingquan Liu, Jiangang Bali, Khaled Thorp, Kelly R. Smith, Richard Wang, Guangyao (Sam) TI Automated Thinning Increases Uniformity of In-row Spacing and Plant Size in Romaine Lettuce SO HORTTECHNOLOGY LA English DT Article DE Lactuca sativa; automated thinner; hand thinning; plant uniformity; lettuce yield AB The low availability and high cost of farm hand labor make automated thinners a faster and cheaper alternative to hand thinning in lettuce (Lactuca-sativa). However, the effects of this new technology on the uniformity of plant spacing and size as well as crop yield are not proven. Three experiments were conducted in commercial romaine heart lettuce fields in 2013 and 2014 in Imperial Valley, CA, to compare the effects of automated thinning and hand thinning on uniformity of in row spacing, plant size, and crop yield. Overhead images taken at 1 week after hand thinning indicate that thinning 8 to 11 days earlier by automated thinners did not affect plant size compared with the hand thinning treatment. However, lettuce plants in the automated thinning treatment were larger than plants in the hand thinning treatment 2 to 3 weeks after hand thinning. Automated thinners increased the uniformity of in-row spacing, increased the percentage of plants with the desired in-row spacing of 24 to 32 cm, and almost completely removed plants with an undesirable in-row spacing of 4 to 20 cm. As a result, individual lettuce plant weight and heart weight from the automated thinning plots was significantly greater and plants were more uniform compared with the hand thinned plants. Despite increases in lettuce plant size and uniformity in all three experiments, yield benefits of automated thinning were only significant in one of the three experiments due to larger plant populations resulting from hand thinning. This study suggests that automated thinning increases lettuce plant size and uniformity and makes it possible for growers to increase plant population and crop yield by optimizing in-row spacing. C1 [Chu, Qingquan; Liu, Jiangang] China Agr Univ, Coll Agron & Biotechnol, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China. [Bali, Khaled] Univ Calif Cooperat Extens, 1050 East Holton Rd, Holtville, CA 92250 USA. [Thorp, Kelly R.] USDA ARS, US Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Smith, Richard] Univ Calif Cooperat Extens, 1432 Abbott St, Salinas, CA 93901 USA. [Wang, Guangyao (Sam)] Shanxi Acad Agr Sci, Inst Cotton Res, 118 Huanghe Rd, Yuncheng 044000, Shanxi, Peoples R China. [Wang, Guangyao (Sam)] Bridgestone Amer, Agr Operat, 4140 West Harmon Rd, Eloy, AZ 85131 USA. RP Wang, GY (reprint author), Shanxi Acad Agr Sci, Inst Cotton Res, 118 Huanghe Rd, Yuncheng 044000, Shanxi, Peoples R China.; Wang, GY (reprint author), Bridgestone Amer, Agr Operat, 4140 West Harmon Rd, Eloy, AZ 85131 USA. EM WangSam@bfusa.com RI Thorp, Kelly/C-2013-2009 OI Thorp, Kelly/0000-0001-9168-875X NR 9 TC 1 Z9 1 U1 3 U2 9 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD FEB PY 2016 VL 26 IS 1 BP 12 EP 19 PG 8 WC Horticulture SC Agriculture GA DE8TK UT WOS:000370909400002 ER PT J AU David, OJ Richard, NO Japheth, I Modesto, OO AF David, Ojwang J. Richard, Nyankanga O. Japheth, Imungi Modesto, Olanya O. TI Plant Characteristics and Growth Parameters of Vegetable Pigeon Pea Cultivars SO HORTTECHNOLOGY LA English DT Article DE Cajanus cajan; plant height; pod development; flowering; maturity; eastern Kenya ID TEMPERATURE; YIELD AB Pigeon pea (Cajanus cajan) is an important crop in dry land and semiarid regions and is a supplementary source of dietary protein for the economic resource constrained farmers. The aim of this research was to evaluate growth parameters of 12 vegetable pigeon pea cultivars at two locations in eastern Kenya. The number of days from planting to flowering, plant height, primary and secondary branches, and pod length and width were quantified in experimental plots in a randomized complete block design with three replications. Significant differences (P < 0.01) in days to 50% and 70% flowering (DTF) and plant maturity (DTM), respectively, were recorded among cultivars at both locations. The average plant height was significantly (P < 0.05) greater at Kiboko than at Kambi ya Mawe. The number of DTF and DTM were also greater at Kiboko than at Kambi ya Mawe site, because of supplemental irrigation. Similarly, mean pod length and width at Kiboko location exceeded that at Kambi ya Mawe by 6% and 8%, respectively. Positive and significant (P < 0.05) correlation coefficients between grain yield and pods per plant were observed, indicating that pod number is a useful indicator of yield potential of vegetable pigeon pea. The cultivars ICEAP 00068, ICEAP 00540, ICEAP 00554, ICEAP 00902, KAT 60/8, and MZ 2/9 were identified for high-yield potential under rain-fed conditions, whereas ICEAP 00902, ICEAP 00068, ICEAP 00557, ICEAP 00554, KAT 60.8, and MTHAWAJUNI showed the greatest potential when supplemental water applications were made. The cultivars KAT 60/8, ICEAP 00068, ICEAP 00554, and ICEAP 00902 were suitable for production under both rain-fed conditions and additional water applications. Yield potential of pigeon pea in the dry regions can be greatly enhanced by using cultivars with good plant growth characteristics and shoot density. C1 [David, Ojwang J.] Solidaridad, POB 42234-00100, Nairobi, Kenya. [Richard, Nyankanga O.] Univ Nairobi, Dept Plant Sci & Crop Protect, POB 30197-00100, Nairobi, Kenya. [Japheth, Imungi] Univ Nairobi, Dept Food Sci Nutr & Technol, POB 30197-00100, Nairobi, Kenya. [Modesto, Olanya O.] USDA ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Modesto, OO (reprint author), USDA ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM modesto.olanya@ars.usda.gov NR 36 TC 0 Z9 0 U1 2 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD FEB PY 2016 VL 26 IS 1 BP 97 EP 105 PG 9 WC Horticulture SC Agriculture GA DE8TK UT WOS:000370909400013 ER PT J AU Shwab, EK Jiang, TT Pena, HFJ Gennari, SM Dubey, JP Su, CL AF Shwab, Elliot Keats Jiang, Tiantian Pena, Hilda F. J. Gennari, Solange M. Dubey, Jitender P. Su, Chunlei TI The ROP18 and ROP5 gene allele types are highly predictive of virulence in mice across globally distributed strains of Toxoplasma gondii SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Virulence; ROP18; ROP5; ROP16; ROP17; Genotyping ID SEVERE ACQUIRED TOXOPLASMOSIS; CONGENITAL TOXOPLASMOSIS; FRENCH-GUIANA; IDENTIFICATION; PSEUDOKINASES; KINASES; FAMILY AB The protozoan parasite Toxoplasma gondii is one of the most successful known eukaryotic pathogens on Earth. Virulence of T. gondii strains varies greatly in mice, and mounting evidence suggests that such variations may be relevant to the manifestation of human toxoplasmosis. Polymorphic rhoptry-secreted kinases and pseudokinases (ROP) have been demonstrated to account for murine virulence among the archetypal clonal parasite lineages that dominate the populations of North America and Europe. However, the distribution of virulence gene alleles in natural populations and the broad influence of these allele combinations on T. gondii virulence have not been examined in depth. In the present study, we performed PCR-RFLP genotyping analysis on a diverse array of globally distributed T. gondii strains at four ROP gene loci including ROP18, ROP5, ROP16 and ROP17 that were previously implicated in influencing T. gondii virulence and pathogenesis. We demonstrated through correlation with published virulence data that the combination of ROP18 and ROP5 allele types is highly predictive of T. gondii virulence across a broad range of global T. gondii isolates. These findings indicate that the importance of ROP18 and ROP5 in determining strain virulence is not limited to the North American/European archetypal lineages most commonly used in molecular studies, but also appears to apply to diverse isolates from South/central America and Asia. Restriction fragment length polymorphism analysis of these loci may thus serve as a valuable tool in determining the potential virulence of uncharacterized T. gondii strains in future studies. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc. C1 [Shwab, Elliot Keats; Jiang, Tiantian; Su, Chunlei] Univ Tennessee, Dept Microbiol, 1414 W Cumberland Ave, Knoxville, TN 37996 USA. [Pena, Hilda F. J.; Gennari, Solange M.] Univ Sao Paulo, Fac Med Vet & Zootecn, Dept Med Vet Preventiva & Sande Anim, Ave Prof Doutor Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil. [Dubey, Jitender P.] USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Agr Res Serv, Beltsville, MD 20705 USA. RP Su, CL (reprint author), Univ Tennessee, Dept Microbiol, 1414 W Cumberland Ave, Knoxville, TN 37996 USA. EM csul@utk.edu OI Su, Chunlei/0000-0001-8392-7108 FU University of Tennessee, Knoxville, USA FX This work was in part supported by the University of Tennessee, Knoxville, USA (C. Su). We are grateful to numerous laboratories that provided T. gondii DNA samples for this study. NR 27 TC 7 Z9 7 U1 2 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 EI 1879-0135 J9 INT J PARASITOL JI Int. J. Parasit. PD FEB PY 2016 VL 46 IS 2 BP 141 EP 146 DI 10.1016/j.ijpara.2015.10.005 PG 6 WC Parasitology SC Parasitology GA DE8RP UT WOS:000370904700008 PM 26699401 ER PT J AU Lessard, SJ Rivas, DA So, K Koh, HJ Queiroz, AL Hirshman, MF Fielding, RA Goodyear, LJ AF Lessard, Sarah J. Rivas, Donato A. So, Kawai Koh, Ho-Jin Queiroz, Andre Lima Hirshman, Michael F. Fielding, Roger A. Goodyear, Laurie J. TI The AMPK-related kinase SNARK regulates muscle mass and myocyte survival SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID DIFFERENT FIBER TYPES; SKELETAL-MUSCLE; SARCOPENIC OBESITY; MYOSIN PHOSPHATASE; LEAN MASS; NUAK2; IDENTIFICATION; MORTALITY; APOPTOSIS; GROWTH AB The maintenance of skeletal muscle mass is critical for sustaining health; however, the mechanisms responsible for muscle loss with aging and chronic diseases, such as diabetes and obesity, are poorly understood. We found that expression of a member of the AMPK-related kinase family, the SNF1-AMPK-related kinase (SNARK, also known as NUAK2), increased with muscle cell differentiation. SNARK expression increased in skeletal muscles from young mice exposed to metabolic stress and in muscles from healthy older human subjects. The regulation of SNARK expression in muscle with differentiation and physiological stress suggests that SNARK may function in the maintenance of muscle mass. Consistent with this hypothesis, decreased endogenous SNARK expression (using siRNA) in cultured muscle cells resulted in increased apoptosis and decreased cell survival under conditions of metabolic stress. Likewise, muscle-specific transgenic animals expressing a SNARK dominant-negative inactive mutant (SDN) had increased myonuclear apoptosis and activation of apoptotic mediators in muscle. Moreover, animals expressing SDN had severe, age-accelerated muscle atrophy and increased adiposity, consistent with sarcopenic obesity. Reduced SNARK activity, in vivo and in vitro, caused downregulation of the Rho kinase signaling pathway, a key mediator of cell survival. These findings reveal a critical role for SNARK in myocyte survival and the maintenance of muscle mass with age. C1 [Lessard, Sarah J.; So, Kawai; Koh, Ho-Jin; Queiroz, Andre Lima; Hirshman, Michael F.; Goodyear, Laurie J.] Brigham & Womens Hosp, Joslin Diabet Ctr, Div Res, 75 Francis St, Boston, MA 02115 USA. [Lessard, Sarah J.; Koh, Ho-Jin; Goodyear, Laurie J.] Brigham & Womens Hosp, Dept Med, 75 Francis St, Boston, MA 02115 USA. [Lessard, Sarah J.; Koh, Ho-Jin; Goodyear, Laurie J.] Harvard Univ, Sch Med, One Joslin Pl, Boston, MA 02215 USA. [Rivas, Donato A.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Goodyear, LJ (reprint author), Brigham & Womens Hosp, Joslin Diabet Ctr, Div Res, 75 Francis St, Boston, MA 02115 USA.; Goodyear, LJ (reprint author), Harvard Univ, Sch Med, One Joslin Pl, Boston, MA 02215 USA. EM laurie.goodyear@joslin.harvard.edu OI Rivas, Donato/0000-0002-4500-6233 FU NIH [AR42238, DK068626, P30 DK036836, P30DK040561]; Joslin Diabetes Center Diabetes Research Center [P30 DK36836]; American Physiological Society (Physiological Genomics); Canadian Diabetes Association; US Department of Agriculture (USDA) [58-1950-0014] FX This work was supported by NIH grants to L.J. Goodyear (AR42238 and DK068626) and the Joslin Diabetes Center Diabetes Research Center (P30 DK36836). H.J. Koh was supported by NIH grants (P30 DK036836 and P30DK040561). S.J. Lessard was supported by postdoctoral fellowships from the American Physiological Society (Physiological Genomics) and the Canadian Diabetes Association. This material is based upon the work supported by the US Department of Agriculture (USDA), under agreement 58-1950-0014. NR 44 TC 3 Z9 3 U1 1 U2 5 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 2015 MANCHESTER RD, ANN ARBOR, MI 48104 USA SN 0021-9738 EI 1558-8238 J9 J CLIN INVEST JI J. Clin. Invest. PD FEB PY 2016 VL 126 IS 2 BP 560 EP 570 DI 10.1172/JCI79197 PG 11 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA DE5NI UT WOS:000370677300018 PM 26690705 ER PT J AU Lulai, EC Suttle, JC Olson, LL Neubauer, JD Campbell, LG Campbell, MA AF Lulai, Edward C. Suttle, Jeffrey C. Olson, Linda L. Neubauer, Jonathan D. Campbell, Larry G. Campbell, Michael A. TI Wounding induces changes in cytokinin and auxin content in potato tuber, but does not induce formation of gibberellins SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Auxin; Cytokinin; Gibberellin; Wound closing-layer; Wound periderm ID ABSCISIC-ACID; ENDOGENOUS GIBBERELLINS; INDUCED SUBERIZATION; PERIDERM FORMATION; SOLANUM-TUBEROSUM; CLOSING LAYER; CIS-ZEATIN; DORMANCY; TISSUE; BIOSYNTHESIS AB Cytokinin, auxin and gibberellin contents in resting and wound-responding potato tubers have not been fully determined and coordinated with wound-healing processes. Using a well-defined wound-healing model system, hormone content and expression of genes associated with hormone turnover were determined in tubers following wounding. Changes in hormone content were coordinated with: (I) formation and completion of the wound closing layer (0-5/6 days), and (II) initiation of phellogen and wound periderm formation (similar to 7 days). Quantifiable amounts of biologically active cytokinins (Z, DZ and IP) were not detected in resting or wound-responding tubers. However, the precursor IPA and catabolic product c-ZOG were found in small amounts in resting and wound-responding tubers. Wound-induced activation of cytokinin biosynthesis was suggested by an increase in t-ZR and c-ZR content at 0.5 days and large increases in IPA and c-ZR content by 3 days and throughout 7 days after wounding suggesting roles in II, but little or no role in I. Expression of key genes involved in cytokinin metabolism followed similar profiles with transcripts decreasing through 3 days and then increasing at 5-7 days after wounding. Both free IAA and IAA-Asp were present in resting tubers. While IAA-Asp was no longer present by 3 days after wounding, IAA content nearly doubled by 5 days and was more than 4-fold greater at 7 days compared to that in resting tuber (0 day) suggesting roles in II, but little or no role in I. Gibberellins were not present in quantifiable amounts in resting or wound-responding tubers. These results suggest that bio-active cytokinins are wound-induced, but their residency is temporal and highly regulated. The transient presence of active cytokinins and corresponding increases in IAA content strongly suggest their involvement in the regulation of wound periderm development. The absence of gibberellins indicates that they are not a regulatory component of wound-healing processes. Published by Elsevier GmbH. C1 [Lulai, Edward C.; Suttle, Jeffrey C.; Olson, Linda L.; Neubauer, Jonathan D.; Campbell, Larry G.] ARS, USDA, Sugarbeet & Potato Unit, No Crop Sci Lab, Fargo, ND 58102 USA. [Campbell, Michael A.] Penn State Univ, Behrend Coll, Penn State Erie, Sch Sci, P-1 Prischak Bldg,4205 Coll Dr, Erie, PA 16563 USA. RP Lulai, EC (reprint author), ARS, USDA, Sugarbeet & Potato Unit, No Crop Sci Lab, Fargo, ND 58102 USA. EM ed.lulai@ars.usda.gov NR 35 TC 3 Z9 3 U1 5 U2 8 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD FEB 1 PY 2016 VL 191 BP 22 EP 28 DI 10.1016/j.jplph.2015.11.006 PG 7 WC Plant Sciences SC Plant Sciences GA DF1YR UT WOS:000371136500003 PM 26708026 ER PT J AU Murdock, JN AF Murdock, Justin N. TI Detecting carbon uptake and cellular allocation by individual algae in multispecies assemblages SO LIMNOLOGY AND OCEANOGRAPHY-METHODS LA English DT Article ID TRANSFORM INFRARED-SPECTROSCOPY; FT-IR SPECTROSCOPY; PHYTOPLANKTON COMMUNITIES; WATER-QUALITY; MICROSPECTROSCOPY; MICROALGAE; TOOL; IDENTIFICATION; BIODIVERSITY; NUTRIENT AB Algal species vary in carbon (C) need and uptake rates. Understanding differences in C uptake and cellular allocation among species from natural communities will bring new insight into many ecosystem process questions including how species changes will alter energy availability and C sequestration in aquatic ecosystems. A major limitation of current methods that measure algal C incorporation is the inability to separate the response of individual species from mixed-species assemblages. I used Fourier-transform infrared microspectroscopy to qualitatively measure inorganic C-13 isotope incorporation into individual algal cells in single species, two species, and natural phytoplankton assemblages. Lateral shifts in spectral peaks from C-13 treatments were observed in all species. Comparison of peaks associated with carbohydrates, proteins, and lipids allowed for the detection of which individuals took in C, and which macromolecules the C was used to make. For example, shifts in Spirogyra spectral peaks showed substantial C incorporation in carbohydrates. Further, shifts in peaks at 1160 cm(-1), 1108 cm(-1), 1080 cm(-1), 1048 cm(-1), and 1030 cm(-1) suggested C was being allocated into cellulose. The natural phytoplankton assemblage demonstrated how C could be tracked into co-occurring species. A diatom had large shifts in protein and carbohydrate peaks, while a green alga and euglenoid had only a few shifts in protein related peaks. Fourier-transform infrared microspectroscopy is an established, label free method for measuring the chemical composition of algal cells. However, adding a label such as C-13 isotope can greatly expand the technique's capabilities by qualitatively tracking C movement between inorganic and organic states within single cells. C1 [Murdock, Justin N.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Murdock, Justin N.] Tennessee Technol Univ, Dept Biol, Cookeville, TN 38505 USA. RP Murdock, JN (reprint author), USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA.; Murdock, JN (reprint author), Tennessee Technol Univ, Dept Biol, Cookeville, TN 38505 USA. EM jnmurdock@tntech.edu FU U.S. Department of Energy [DE-AC02-98CH10886]; U.S. Department of Agriculture, Agricultural Research Service FX I thank Ruvini Omattage for laboratory assistance, and K. Henderson and N. Knorp provided comments that improved this manuscript. J. Rimoldi provided use of the benchtop FTIR microscope. I also thank L. Miller for facilitating the use of synchrotron infrared beamline U2B. The National Synchrotron Light Source of Brookhaven National Laboratory, Upton, NY, is operated as a user facility by the U.S. Department of Energy under contract no. DE-AC02-98CH10886. This work was funded by the U.S. Department of Agriculture, Agricultural Research Service. NR 39 TC 0 Z9 0 U1 5 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1541-5856 J9 LIMNOL OCEANOGR-METH JI Limnol. Oceanogr. Meth. PD FEB PY 2016 VL 14 IS 2 BP 124 EP 137 DI 10.1002/lom3.10078 PG 14 WC Limnology; Oceanography SC Marine & Freshwater Biology; Oceanography GA DF2IW UT WOS:000371167100007 ER PT J AU McClatchy, DB Savas, JN Martinez-Bartolome, S Park, SK Maher, P Powell, SB Yates, JR AF McClatchy, D. B. Savas, J. N. Martinez-Bartolome, S. Park, S. K. Maher, P. Powell, S. B. Yates, J. R., III TI Global quantitative analysis of phosphorylation underlying phencyclidine signaling and sensorimotor gating in the prefrontal cortex SO MOLECULAR PSYCHIATRY LA English DT Article ID INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; KINASE-ASSOCIATED PROTEIN; PREPULSE INHIBITION; SYNAPTIC PLASTICITY; MASS-SPECTROMETRY; GENE-EXPRESSION; MULTISITE PHOSPHORYLATION; SYSTEM X(C)(-); STARTLE REFLEX; CELL-CYCLE AB Prepulse inhibition (PPI) is an example of sensorimotor gating and deficits in PPI have been demonstrated in schizophrenia patients. Phencyclidine (PCP) suppression of PPI in animals has been studied to elucidate the pathological elements of schizophrenia. However, the molecular mechanisms underlying PCP treatment or PPI in the brain are still poorly understood. In this study, quantitative phosphoproteomic analysis was performed on the prefrontal cortex from rats that were subjected to PPI after being systemically injected with PCP or saline. PCP downregulated phosphorylation events were significantly enriched in proteins associated with long-term potentiation (LTP). Importantly, this data set identifies functionally novel phosphorylation sites on known LTP-associated signaling molecules. In addition, mutagenesis of a significantly altered phosphorylation site on xCT (SLC7A11), the light chain of system xc-, the cystine/glutamate antiporter, suggests that PCP also regulates the activity of this protein. Finally, new insights were also derived on PPI signaling independent of PCP treatment. This is the first quantitative phosphorylation proteomic analysis providing new molecular insights into sensorimotor gating. C1 [McClatchy, D. B.; Savas, J. N.; Martinez-Bartolome, S.; Park, S. K.; Yates, J. R., III] Scripps Res Inst, Dept Physiol Chem, 10550 North Torrey Pines SR11, La Jolla, CA 92037 USA. [Maher, P.] Salk Inst Biol Studies, Cellular Neurobiol Lab, La Jolla, CA USA. [Powell, S. B.] USDA, Dept Psychiat, La Jolla, CA USA. RP Yates, JR (reprint author), Scripps Res Inst, Dept Physiol Chem, 10550 North Torrey Pines SR11, La Jolla, CA 92037 USA. EM jyates@scripps.edu OI Martinez de Bartolome, Salvador/0000-0001-7592-5612 FU National Institutes of Health [P41 GM103533, R01 MH067880, R01 DA002925] FX Funding has been provided by National Institutes of Health grants P41 GM103533, R01 MH067880 and R01 DA002925. NR 116 TC 0 Z9 0 U1 3 U2 4 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1359-4184 EI 1476-5578 J9 MOL PSYCHIATR JI Mol. Psychiatr. PD FEB PY 2016 VL 21 IS 2 BP 205 EP 215 DI 10.1038/mp.2015.41 PG 11 WC Biochemistry & Molecular Biology; Neurosciences; Psychiatry SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Psychiatry GA DE7LI UT WOS:000370817800007 PM 25869802 ER PT J AU Benoit, JB Adelman, ZN Reinhardt, K Dolan, A Poelchau, M Jennings, EC Szuter, EM Hagan, RW Gujar, H Shukla, JN Zhu, F Mohan, M Nelson, DR Rosendale, AJ Derst, C Resnik, V Wernig, S Menegazzi, P Wegener, C Peschel, N Hendershot, JM Blenau, W Predel, R Johnston, PR Ioannidis, P Waterhouse, RM Nauen, R Schorn, C Ott, MC Maiwald, F Johnston, JS Gondhalekar, AD Scharf, ME Peterson, BF Raje, KR Hottel, BA Armisen, D Crumiere, AJJ Refki, PN Santos, ME Sghaier, E Viala, S Khila, A Ahn, SJ Childers, C Lee, CY Lin, H Hughes, DST Duncan, EJ Murali, SC Qu, JX Dugan, S Lee, SL Chao, H Dinh, H Han, Y Doddapaneni, H Worley, KC Muzny, DM Wheeler, D Panfilio, KA Jentzsch, IMV Vargo, EL Booth, W Friedrich, M Weirauch, MT Anderson, MAE Jones, JW Mittapalli, O Zhao, CY Zhou, JJ Evans, JD Attardo, GM Robertson, HM Zdobnov, EM Ribeiro, JMC Gibbs, RA Werren, JH Palli, SR Schal, C Richards, S AF Benoit, Joshua B. Adelman, Zach N. Reinhardt, Klaus Dolan, Amanda Poelchau, Monica Jennings, Emily C. Szuter, Elise M. Hagan, Richard W. Gujar, Hemant Shukla, Jayendra Nath Zhu, Fang Mohan, M. Nelson, David R. Rosendale, Andrew J. Derst, Christian Resnik, Valentina Wernig, Sebastian Menegazzi, Pamela Wegener, Christian Peschel, Nicolai Hendershot, Jacob M. Blenau, Wolfgang Predel, Reinhard Johnston, Paul R. Ioannidis, Panagiotis Waterhouse, Robert M. Nauen, Ralf Schorn, Corinna Ott, Mark-Christoph Maiwald, Frank Johnston, J. Spencer Gondhalekar, Ameya D. Scharf, Michael E. Peterson, Brittany F. Raje, Kapil R. Hottel, Benjamin A. Armisen, David Crumiere, Antonin Jean Johan Refki, Peter Nagui Santos, Maria Emilia Sghaier, Essia Viala, Severine Khila, Abderrahman Ahn, Seung-Joon Childers, Christopher Lee, Chien-Yueh Lin, Han Hughes, Daniel S. T. Duncan, Elizabeth J. Murali, Shwetha C. Qu, Jiaxin Dugan, Shannon Lee, Sandra L. Chao, Hsu Dinh, Huyen Han, Yi Doddapaneni, Harshavardhan Worley, Kim C. Muzny, Donna M. Wheeler, David Panfilio, Kristen A. Jentzsch, Iris M. Vargas Vargo, Edward L. Booth, Warren Friedrich, Markus Weirauch, Matthew T. Anderson, Michelle A. E. Jones, Jeffery W. Mittapalli, Omprakash Zhao, Chaoyang Zhou, Jing-Jiang Evans, Jay D. Attardo, Geoffrey M. Robertson, Hugh M. Zdobnov, Evgeny M. Ribeiro, Jose M. C. Gibbs, Richard A. Werren, John H. Palli, Subba R. Schal, Coby Richards, Stephen TI Unique features of a global human ectoparasite identified through sequencing of the bed bug genome SO NATURE COMMUNICATIONS LA English DT Article ID CIMEX-LECTULARIUS L.; INSECTICIDE RESISTANCE; TRAUMATIC INSEMINATION; HEMIPTERA-CIMICIDAE; RHODNIUS-PROLIXUS; PROTEIN GENES; R CONSENSUS; ARTHROPODS; DROSOPHILA; RECEPTORS AB The bed bug, Cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. This global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. Analyses of the C. lectularius sequenced genome (650Mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory repertoire of genes related to obligate hematophagy, host-symbiont interactions, and several mechanisms of insecticide resistance. In addition, we document the presence of multiple putative lateral gene transfer events. Genome sequencing and annotation establish a solid foundation for future research on mechanisms of insecticide resistance, human-bed bug and symbiont-bed bug associations, and unique features of bed bug biology that contribute to the unprecedented success of C. lectularius as a human ectoparasite. C1 [Benoit, Joshua B.; Jennings, Emily C.; Szuter, Elise M.; Hagan, Richard W.; Rosendale, Andrew J.; Hendershot, Jacob M.] Univ Cincinnati, Dept Biol Sci, Cincinnati, OH 45221 USA. [Adelman, Zach N.; Anderson, Michelle A. E.] Virginia Tech, Fralin Life Sci Inst, Blacksburg, VA 24061 USA. [Adelman, Zach N.; Anderson, Michelle A. E.] Virginia Tech, Dept Entomol, Blacksburg, VA 24061 USA. [Reinhardt, Klaus] Tech Univ Dresden, Dept Biol, Appl Zool, D-01062 Dresden, Germany. [Dolan, Amanda; Werren, John H.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA. [Poelchau, Monica; Childers, Christopher; Lee, Chien-Yueh; Lin, Han] Natl Agr Lib, Beltsville, MD 20705 USA. [Gujar, Hemant; Shukla, Jayendra Nath; Zhu, Fang; Palli, Subba R.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. [Zhu, Fang] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Mohan, M.] Indian Council Agr Res, ICAR Natl Bur Agr Insect Resources, Bengaluru 560024, Karnataka, India. [Nelson, David R.] Univ Tennessee, Ctr Hlth Sci, Dept Microbiol Immunol & Biochem, Memphis, TN 38163 USA. [Derst, Christian; Blenau, Wolfgang; Predel, Reinhard] Univ Cologne, Cologne Bioctr, D-50674 Cologne, Germany. [Derst, Christian; Blenau, Wolfgang; Predel, Reinhard] Univ Cologne, Inst Zool, D-50674 Cologne, Germany. [Resnik, Valentina; Wernig, Sebastian] Goethe Univ Frankfurt, Inst Bienenkunde, Polytech Gesell, D-61440 Oberursel, Germany. [Menegazzi, Pamela; Wegener, Christian; Peschel, Nicolai] Univ Wurzburg, Bioctr, Theodor Boveri Inst, Dept Neurobiol & Genet, D-97074 Wurzburg, Germany. [Johnston, Paul R.] Free Univ Berlin, Inst Biol, Dept Evolutionary Biol, D-14195 Berlin, Germany. [Johnston, J. Spencer; Vargo, Edward L.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Ioannidis, Panagiotis; Waterhouse, Robert M.; Zdobnov, Evgeny M.] Univ Geneva, Dept Genet Med & Dev, CH-1211 Geneva, Switzerland. [Ioannidis, Panagiotis; Waterhouse, Robert M.; Zdobnov, Evgeny M.] Univ Geneva, Swiss Inst Bioinformat, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.] Massachusetts Inst Technol & Broad Inst MIT & Har, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA. [Nauen, Ralf; Schorn, Corinna; Ott, Mark-Christoph; Maiwald, Frank] Bayer CropSci AG, Pest Control Biol & Res Technol, D-40789 Monheim, Germany. [Gondhalekar, Ameya D.; Scharf, Michael E.; Peterson, Brittany F.; Raje, Kapil R.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Hottel, Benjamin A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Armisen, David; Crumiere, Antonin Jean Johan; Refki, Peter Nagui; Santos, Maria Emilia; Sghaier, Essia; Viala, Severine; Khila, Abderrahman] Ecole Normale Super Lyon, CNRS UMR5242, IGFL, F-69007 Lyon, France. [Ahn, Seung-Joon] Max Planck Inst Chem Ecol, Dept Entomol, D-07745 Jena, Germany. [Lee, Chien-Yueh; Lin, Han] Natl Taiwan Univ, Grad Inst Biomed Elect & Bioinformat, Taipei 10617, Taiwan. [Hughes, Daniel S. T.; Murali, Shwetha C.; Qu, Jiaxin; Dugan, Shannon; Lee, Sandra L.; Chao, Hsu; Dinh, Huyen; Han, Yi; Doddapaneni, Harshavardhan; Worley, Kim C.; Muzny, Donna M.; Gibbs, Richard A.; Richards, Stephen] Baylor Coll Med, Human Genome Sequencing Ctr, Dept Human & Mol Genet, Houston, TX 77030 USA. [Duncan, Elizabeth J.] Univ Otago, Dept Biochem & Genet Otago, Dunedin 9054, New Zealand. [Wheeler, David] Massey Univ, Inst Fundamental Sci, Palmerston North 4442, New Zealand. [Panfilio, Kristen A.; Jentzsch, Iris M. Vargas] Univ Cologne, Inst Dev Biol, D-50674 Cologne, Germany. [Booth, Warren] Univ Tulsa, Dept Biol Sci, Tulsa, OK 74104 USA. [Friedrich, Markus; Jones, Jeffery W.] Wayne State Univ, Dept Biol Sci, Detroit, MI 48202 USA. [Weirauch, Matthew T.] Univ Cincinnati, Ctr Autoimmune Genom & Etiol, Div Biomed Informat, Cincinnati, OH 45229 USA. [Weirauch, Matthew T.] Univ Cincinnati, Cincinnati Childrens Hosp Med Ctr, Div Dev Biol, Dept Pediat,Coll Med, Cincinnati, OH 45229 USA. [Mittapalli, Omprakash; Zhao, Chaoyang] Ohio State Univ, Dept Entomol, Wooster, OH 44691 USA. [Zhou, Jing-Jiang] BBSRC, Rothamsted Res, Dept Biol Chem & Crop Protect, Harpenden AL5 2JQ, Herts, England. [Evans, Jay D.] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Attardo, Geoffrey M.] Yale Univ, Yale Sch Publ Hlth, Dept Epidemiol Microbial Dis, New Haven, CT 06520 USA. [Robertson, Hugh M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Ribeiro, Jose M. C.] NIAID, Lab Malaria & Vector Res, Bethesda, MD 20892 USA. [Schal, Coby] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Schal, Coby] N Carolina State Univ, WM Keck Ctr Behav Biol, Raleigh, NC 27695 USA. RP Benoit, JB (reprint author), Univ Cincinnati, Dept Biol Sci, Cincinnati, OH 45221 USA.; Richards, S (reprint author), Baylor Coll Med, Human Genome Sequencing Ctr, Dept Human & Mol Genet, Houston, TX 77030 USA. EM joshua.benoit@uc.edu; stephenr@bcm.edu RI Blenau, Wolfgang/A-4304-2010; Waterhouse, Robert/A-1858-2010; Zhu, Fang/L-3411-2016; Schal, Coby/A-8717-2010; Zdobnov, Evgeny/K-1133-2012; Ioannidis, Panagiotis/I-4281-2014; OI Childers, Chris/0000-0002-1253-5550; Panfilio, Kristen/0000-0002-6417-251X; Menegazzi, Pamela/0000-0001-5232-1920; Evans, Jay/0000-0002-0036-4651; Ribeiro, Jose/0000-0002-9107-0818; Duncan, Elizabeth/0000-0002-1841-504X; Waterhouse, Robert/0000-0003-4199-9052; Schal, Coby/0000-0001-7195-6358; Wegener, Christian/0000-0003-4481-3567; Ioannidis, Panagiotis/0000-0003-0939-6745; Adelman, Zach/0000-0001-5901-7171; Khila, Abderrahman/0000-0003-0908-483X; Ahn, Seung-Joon/0000-0002-5980-106X FU NHGRI grant [U54 HG003273]; Blanton J. Whitmire endowment, Housing and Urban Development [NCHHU-00017-13]; National Science Foundation [IOS-1052238, DEB-1257053]; Alfred P. Sloan Foundation [2013-5-35 MBE]; NIH [GM070559-9]; Royal Society of New Zealand Marsden Fast Start Grant [11-UOO-124]; Fralin Life Sciences Institutes and Virginia Agriculture Experimental Station; European Research Council [ERC-CoG 616346, 260986]; Deutsche Forschungsgemeinschaft (DFG) [Pe1798/2-1, PA2044/1-1, BL469/7-1, 766/11-1]; Exzellenzinitiative of the Deutsche Forschungsgemeinschaft; Biotechnology and Biological Sciences Research Council; University of Cincinnati Faculty Development Research Grant; Ohio Supercomputer Center Research Allocation; Marie Curie International Outgoing Fellowship [PIOF-GA-2011-303312]; Swiss National Science Foundation [31003A-125350, 31003A-143936]; Deutsche Forschungsgemeinschaft (DFG): collaborative research center [SFB 1047] FX Funding for genome sequencing, assembly and automated annotation was provided by NHGRI grant U54 HG003273 to R.A.G. Funding for bed bug lines development was provided by Blanton J. Whitmire endowment, Housing and Urban Development (NCHHU-00017-13), National Science Foundation (IOS-1052238), Alfred P. Sloan Foundation (2013-5-35 MBE) to Cob. S. Funding for preparation of genomic DNA and RNA for sequencing and for manual curation of some of the genes was provided by NIH (GM070559-9) to S.R.P. Funding for manual curation and other analyses were provided by the Royal Society of New Zealand Marsden Fast Start Grant (11-UOO-124) to E.J.D., Fralin Life Sciences Institutes and Virginia Agriculture Experimental Station to Z.N.A., European Research Council (ERC-CoG 616346) to A.K., Deutsche Forschungsgemeinschaft (DFG): Pe1798/2-1 to N.P., Deutsche Forschungsgemeinschaft (DFG): PA2044/1-1 to K.A.P., Deutsche Forschungsgemeinschaft (DFG): collaborative research center SFB 1047 `Insect timing,' Project A1 to P.M., The Zukunftskonzept at TU Dresden funded by the Exzellenzinitiative of the Deutsche Forschungsgemeinschaft to K.R., Deutsche Forschungsgemeinschaft (DFG): BL469/7-1 to Wo.B., Deutsche Forschungsgemeinschaft (DFG): 766/11-1 to R.P., European Research Council grant 260986 to P. J., Biotechnology and Biological Sciences Research Council to J.-J.Z., University of Cincinnati Faculty Development Research Grant and Ohio Supercomputer Center Research Allocation to J.B.B., Marie Curie International Outgoing Fellowship PIOF-GA-2011-303312 to R.M.W., National Science Foundation (DEB-1257053) to J.H.W., and Swiss National Science Foundation awards 31003A-125350 and 31003A-143936 to E.M.Z. We thank Rick Santangelo for generating the full-sib mating line used in this project. Given the restriction in the number of citations, it was not possible include all publications associated with programs used in this study. As an alternative, we have provided links to websites for each program. i5k genome sequencing initiative assisting in the sequencing of the bed bug genome. NR 70 TC 12 Z9 13 U1 15 U2 42 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD FEB PY 2016 VL 7 AR 10165 DI 10.1038/ncomms10165 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF0EC UT WOS:000371010800002 PM 26836814 ER PT J AU Korhonen, PK Pozio, E La Rosa, G Chang, BCH Koehler, AV Hoberg, EP Boag, PR Tan, P Jex, AR Hofmann, A Sternberg, PW Young, ND Gasser, RB AF Korhonen, Pasi K. Pozio, Edoardo La Rosa, Giuseppe Chang, Bill C. H. Koehler, Anson V. Hoberg, Eric P. Boag, Peter R. Tan, Patrick Jex, Aaron R. Hofmann, Andreas Sternberg, Paul W. Young, Neil D. Gasser, Robin B. TI Phylogenomic and biogeographic reconstruction of the Trichinella complex SO NATURE COMMUNICATIONS LA English DT Article ID BOUNDARY STRATOTYPE SECTION; HUMAN TRICHINOSIS; DRAFT GENOME; CYSTATIN SUPERFAMILY; CARNIVOROUS MAMMALS; NEMATODE PARASITE; GROWTH-FACTOR; LAND-BRIDGE; POINT GSSP; RNA-SEQ AB Trichinellosis is a globally important food-borne parasitic disease of humans caused by roundworms of the Trichinella complex. Extensive biological diversity is reflected in substantial ecological and genetic variability within and among Trichinella taxa, and major controversy surrounds the systematics of this complex. Here we report the sequencing and assembly of 16 draft genomes representing all 12 recognized Trichinella species and genotypes, define protein-coding gene sets and assess genetic differences among these taxa. Using thousands of shared single-copy orthologous gene sequences, we fully reconstruct, for the first time, a phylogeny and biogeography for the Trichinella complex, and show that encapsulated and non-encapsulated Trichinella taxa diverged from their most recent common ancestor similar to 21 million years ago (mya), with taxon diversifications commencing similar to 10 - 7 mya. C1 [Korhonen, Pasi K.; Chang, Bill C. H.; Koehler, Anson V.; Jex, Aaron R.; Hofmann, Andreas; Young, Neil D.; Gasser, Robin B.] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia. [Pozio, Edoardo; La Rosa, Giuseppe] Ist Super Sanita, Viale Regina Elena 299, I-00161 Rome, Italy. [Chang, Bill C. H.] Yourgene Biosci, New Taipei 23863, Taiwan. [Hoberg, Eric P.] ARS, US Natl Parasite Collect, USDA, Beltsville, MD 20705 USA. [Boag, Peter R.] Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic 3800, Australia. [Tan, Patrick] Genome Inst Singapore, 60 Biopolis St, Singapore 138672, Singapore. [Tan, Patrick] Duke NUS Grad Med Sch, Canc & Stem Cell Biol, Singapore 138672, Singapore. [Hofmann, Andreas] Griffith Univ, Eskitis Inst, Struct Chem Program, Brisbane, Qld 4111, Australia. [Sternberg, Paul W.] CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA. RP Gasser, RB (reprint author), Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia. EM robinbg@unimelb.edu.au RI Hofmann, Andreas/B-9515-2008; OI Hofmann, Andreas/0000-0003-4408-5467; Korhonen, Pasi/0000-0002-9957-4674; Koehler, Anson/0000-0001-8330-6416 FU Australian Research Council; National Health and Medical Research Council (NHMRC) of Australia; Yourgene Bioscience; Melbourne Water Corporation; DG SANCO of the European Commission; Istituto Superiore di Sanita, Rome; Victorian Life Sciences Computation Initiative [VR0007]; University of Melbourne FX Funding from the Australian Research Council, the National Health and Medical Research Council (NHMRC) of Australia, Yourgene Bioscience and Melbourne Water Corporation is gratefully acknowledged (R.B.G. et al.). This project was funded in part through DG SANCO of the European Commission and Istituto Superiore di Sanita, Rome (E.P.). This project was also supported by a Victorian Life Sciences Computation Initiative (grant number VR0007) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government (R.B.G.). N.D.Y. holds an NHMRC Early Career Research Fellowship. P.W.S. thanks the Howard Hughes Medical Institute (HHMI) and the National Institutes of Health (NIH). We thank the staff of Yourgene Bioscience for their contributions. We would specifically like to acknowledge all scientists who developed the programmes used in this study. Given restrictions on the number of articles that could be cited, we were unable to include all original articles in the Methods section. Instead, we have included links to respective websites. P.K.K. is the recipient of a scholarship (STRAPA) from the University of Melbourne. NR 75 TC 4 Z9 4 U1 8 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD FEB PY 2016 VL 7 AR 10513 DI 10.1038/ncomms10513 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF0EV UT WOS:000371012700001 PM 26830005 ER PT J AU McCouch, SR Wright, MH Tung, CW Maron, LG McNally, KL Fitzgerald, M Singh, N DeClerck, G Agosto-Perez, F Korniliev, P Greenberg, AJ Naredo, MEB Mercado, SMQ Harrington, SE Shi, YX Branchini, DA Kuser-Falcao, PR Leung, H Ebana, K Yano, M Eizenga, G McClung, A Mezey, J AF McCouch, Susan R. Wright, Mark H. Tung, Chih-Wei Maron, Lyza G. McNally, Kenneth L. Fitzgerald, Melissa Singh, Namrata DeClerck, Genevieve Agosto-Perez, Francisco Korniliev, Pavel Greenberg, Anthony J. Naredo, Ma. Elizabeth B. Mercado, Sheila Mae Q. Harrington, Sandra E. Shi, Yuxin Branchini, Darcy A. Kuser-Falcao, Paula R. Leung, Hei Ebana, Kowaru Yano, Masahiro Eizenga, Georgia McClung, Anna Mezey, Jason TI Open access resources for genome-wide association mapping in rice SO NATURE COMMUNICATIONS LA English DT Article ID ORYZA-SATIVA; GRAIN LENGTH; POPULATION-STRUCTURE; CULTIVATED RICE; PLANT HEIGHT; DWARF MUTANT; SEED LENGTH; MAJOR QTL; GENE; PROTEIN AB Increasing food production is essential to meet the demands of a growing human population, with its rising income levels and nutritional expectations. To address the demand, plant breeders seek new sources of genetic variation to enhance the productivity, sustainability and resilience of crop varieties. Here we launch a high-resolution, open-access research platform to facilitate genome-wide association mapping in rice, a staple food crop. The platform provides an immortal collection of diverse germplasm, a high-density single-nucleotide polymorphism data set tailored for gene discovery, well-documented analytical strategies, and a suite of bioinformatics resources to facilitate biological interpretation. Using grain length, we demonstrate the power and resolution of our new high-density rice array, the accompanying genotypic data set, and an expanded diversity panel for detecting major and minor effect QTLs and subpopulation-specific alleles, with immediate implications for rice improvement. C1 [McCouch, Susan R.; Wright, Mark H.; Tung, Chih-Wei; Maron, Lyza G.; Singh, Namrata; DeClerck, Genevieve; Agosto-Perez, Francisco; Korniliev, Pavel; Greenberg, Anthony J.; Harrington, Sandra E.; Shi, Yuxin; Kuser-Falcao, Paula R.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet sect, Ithaca, NY 14850 USA. [McCouch, Susan R.; Agosto-Perez, Francisco; Korniliev, Pavel; Greenberg, Anthony J.; Mezey, Jason] Cornell Univ, Dept Biol Stat & Computat Biol, Ithaca, NY 14850 USA. [McNally, Kenneth L.; Fitzgerald, Melissa; Naredo, Ma. Elizabeth B.; Mercado, Sheila Mae Q.; Leung, Hei] Int Rice Res Inst, DAPO Box 7777,1301 Metro, Manila, Philippines. [Fitzgerald, Melissa] Univ Queensland, Sch Food Sci, St Lucia, Qld 4072, Australia. [Branchini, Darcy A.] Cornell Univ, Transnat Learning Ctr, Ithaca, NY 14850 USA. [Ebana, Kowaru; Yano, Masahiro] Natl Inst Agrobiol Sci, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan. [Eizenga, Georgia; McClung, Anna] USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130 E, Stuttgart, AR 72160 USA. [Wright, Mark H.] Stanford Sch Med, Dept Genet, Stanford, CA 94305 USA. [Tung, Chih-Wei] Natl Taiwan Univ, Dept Agron, Taipei 106, Taiwan. [Branchini, Darcy A.] Cornell Univ, Mann Lib, Ithaca, NY 14850 USA. [Kuser-Falcao, Paula R.] Embrapa Agr Informat, BR-13083886 Campinas, Brazil. RP McCouch, SR (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet sect, Ithaca, NY 14850 USA.; McCouch, SR (reprint author), Cornell Univ, Dept Biol Stat & Computat Biol, Ithaca, NY 14850 USA. EM srm4@cornell.edu RI Maron, Lyza/B-5644-2008; OI Maron, Lyza/0000-0001-6475-2357; Wright, Mark/0000-0001-8036-1161; TUNG, CHIH-WEI/0000-0001-5337-0177 FU United States Department of Agriculture (USDA AFRI award) [2009-01881]; US- National Science Foundation (NSF-PGRP) [0606461, 1026555]; Japan-World Bank Breeding project [DRPC2008-112]; Bayer and Syngenta SKEP projects [DRPC2008-108, DRPC2009-101] FX We gratefully acknowledge outstanding technical support from Fumio Onishi and Eileen Ceconi at Cornell, Teresa Hancock and Daniel Wood at the Dale Bumpers National Rice Research Center in Stuttgart, AR, USA, the molecular, conservation and field teams of the T.T. Chang Genetic Resources Center and the Grain Quality and Nutrition Center at IRRI, and valuable input during design of the HDRA from Elhan Ersoz. Funding was provided by the United States Department of Agriculture (USDA AFRI award #2009-01881 to SMc); the US-National Science Foundation (NSF-PGRP awards #0606461 and #1026555 to SMc), Japan-World Bank Breeding project (DRPC2008-112 to IRRI), Bayer and Syngenta SKEP projects (DRPC2008-108 and DRPC2009-101 to IRRI). NR 68 TC 20 Z9 23 U1 10 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD FEB PY 2016 VL 7 AR 10532 DI 10.1038/ncomms10532 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF0EZ UT WOS:000371013100001 PM 26842267 ER PT J AU Verma, SK Carstensen, M Calero-Bernal, R Moore, SA Jiang, TT Su, CL Dubey, JP AF Verma, Shiv K. Carstensen, Michelle Calero-Bernal, Rafael Moore, Seth A. Jiang, Tiantian Su, Chunlei Dubey, Jitender P. TI Seroprevalence, isolation, first genetic characterization of Toxoplasma gondii, and possible congenital transmission in wild moose from Minnesota, USA SO PARASITOLOGY RESEARCH LA English DT Article DE Toxoplasma gondii; Moose; Bioassay; Genotype; Congenital ID PREVALENCE; ALASKA; DEER; EPIDEMIOLOGY; ANTIBODIES; INFECTION AB Toxoplasma gondii infections are widespread in white-tailed deer (Odocoileus virginianus) but little is known of its prevalence in other cervids in the USA. Moose (Alces alces) is a popular large game animal, hunted for its meat and trophy antlers. Here, we report seroprevalence, isolation, and genetic characterization of T. gondii from moose from Minnesota. Antibodies against T. gondii were detected in 8 of 79 (10 %) moose tested by the modified agglutination test (MAT 1:25 or higher). The myocardium of 68 moose was bioassayed individually in mice, irrespective of serological status. T. gondii was detected in three moose (2 adults, 1 3 weeks old). The parasite from 2 adults was further propagated in cell culture. PCR-RFLP genotyping of cell culture derived tachyzoites using 10 genetic markers, SAG1, SAG2 (5' and 3' SAG2, and alt. SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed two different ToxoDB PCR-RFLP genotypes (#5, designated TgMooseUS1, and #7, TgMooseUS2). The mice inoculated with myocardium of the juvenile moose developed antibodies against T. gondii, and DNA extracted from infected mouse brain was only partially characterized by PCR-RFLP genotyping, which suggests a potential new genotype. Result documented prevalence of T. gondii in moose, and its possible transplacental/transmammary transmission of T. gondii in moose. C1 [Verma, Shiv K.; Calero-Bernal, Rafael; Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. [Carstensen, Michelle] Minnesota Dept Nat Resource, Div Wildlife, 5463-C West Broadway, Forest Lake, MN 55056 USA. [Moore, Seth A.] Dept Biol & Environm Grand Portage Band Chippewa, Portage, MN 55605 USA. [Jiang, Tiantian; Su, Chunlei] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 24 TC 1 Z9 1 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD FEB PY 2016 VL 115 IS 2 BP 687 EP 690 DI 10.1007/s00436-015-4789-0 PG 4 WC Parasitology SC Parasitology GA DE8EO UT WOS:000370868800026 PM 26472716 ER PT J AU Govindarajan, M Rajeswary, M Veerakumar, K Muthukumaran, U Hoti, SL Mehlhorn, H Barnard, DR Benelli, G AF Govindarajan, Marimuthu Rajeswary, Mohan Veerakumar, Kaliyan Muthukumaran, Udaiyan Hoti, S. L. Mehlhorn, Heinz Barnard, Donald R. Benelli, Giovanni TI Novel synthesis of silver nanoparticles using Bauhinia variegata: a recent eco-friendly approach for mosquito control SO PARASITOLOGY RESEARCH LA English DT Article DE Arbovirus; Eco-friendly larvicide; Japanese encephalitis; Malaria; Mosquito-borne diseases; Nanotechnology ID ANOPHELES-STEPHENSI LISTON; ALBOPICTUS DIPTERA-CULICIDAE; AEDES-AEGYPTI LARVAE; VECTOR CULEX-PIPIENS; LARVICIDAL ACTIVITY; ESSENTIAL OILS; CHEMICAL-COMPOSITION; LEAF EXTRACT; MALARIAL VECTOR; GREEN SYNTHESIS AB Mosquito vectors are responsible for transmitting diseases such as malaria, dengue, chikungunya, Japanese encephalitis, dengue, and lymphatic filariasis. The use of synthetic insecticides to control mosquito vectors has caused physiological resistance and adverse environmental effects, in addition to high operational cost. Biosynthesis of silver nanoparticles has been proposed as an alternative to traditional control tools. In the present study, green synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Bauhinia variegata by reduction of Ag+ ions from silver nitrate solution has been investigated. The bioreduced silver nanoparticles were characterized by UV-visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray analysis (EDX), and Xray diffraction analysis (XRD). Leaf extract and synthesized AgNPs were evaluated against the larvae of Anopheles subpictus, Aedes albopictus, and Culex tritaeniorhynchus. Compared to aqueous extract, synthesized AgNPs showed higher toxicity against An. subpictus, Ae. albopictus, and Cx. tritaeniorhynchus with LC50 and LC90 values of 41.96, 46.16, and 51.92 mu g/mL and 82.93, 89.42, and 97.12 mu g/mL, respectively. Overall, this study proves that B. variegata is a potential bioresource for stable, reproducible nanoparticle synthesis and may be proposed as an efficient mosquito control agent. C1 [Govindarajan, Marimuthu; Rajeswary, Mohan; Veerakumar, Kaliyan; Muthukumaran, Udaiyan] Annamalai Univ, Dept Zool, Unit Vector Control Phytochem & Nanotechnol, Annamalainagar 608002, Tamil Nadu, India. [Hoti, S. L.] Reg Med Res Ctr, Belgaum 590010, Karnataka, India. [Mehlhorn, Heinz] Univ Dusseldorf, Dept Parasitol, Dusseldorf, Germany. [Barnard, Donald R.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. [Benelli, Giovanni] Univ Pisa, Dept Agr Food & Environm, Via Borghetto 80, I-56124 Pisa, Italy. RP Govindarajan, M (reprint author), Annamalai Univ, Dept Zool, Unit Vector Control Phytochem & Nanotechnol, Annamalainagar 608002, Tamil Nadu, India.; Benelli, G (reprint author), Univ Pisa, Dept Agr Food & Environm, Via Borghetto 80, I-56124 Pisa, Italy. EM drgovindzoo@yahoo.com; benelli.giovanni@gmail.com RI Benelli, Giovanni/A-9100-2013; OI Benelli, Giovanni/0000-0001-8971-6010; govindarajan, marimuthu/0000-0002-4662-8931 NR 76 TC 22 Z9 22 U1 9 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD FEB PY 2016 VL 115 IS 2 BP 723 EP 733 DI 10.1007/s00436-015-4794-3 PG 11 WC Parasitology SC Parasitology GA DE8EO UT WOS:000370868800031 PM 26490683 ER PT J AU Kogut, MH Genovese, KJ He, HQ Arsenault, RJ AF Kogut, Michael H. Genovese, Kenneth J. He, Haiqi Arsenault, Ryan J. TI AMPK and mTOR: sensors and regulators of immunometabolic changes during Salmonella infection in the chicken SO POULTRY SCIENCE LA English DT Article DE Salmonella; immunometabolism; immunity; kinome ID ENTERICA SEROVAR TYPHIMURIUM; IMMUNE-RESPONSES; PROTECTIVE IMMUNITY; BACTERIAL PATHOGENS; CELL PROPERTIES; PROTEIN-KINASE; FATTY-ACID; PPAR-DELTA; IN-VIVO; ENTERITIDIS AB Non-typhoidal Salmonella enterica induce an early pro-inflammatory response in chickens, but the response is short-lived, asymptomatic of clinical disease, results in a persistent colonization of the gastrointestinal (GI) tract, and can transmit infections to naive hosts via fecal shedding of bacteria. The underlying mechanisms that facilitate this persistent colonization of the ceca of chickens by Salmonella are unknown. We have begun to concentrate on the convergence of metabolism and immune function as playing a major role in regulating the host responsiveness to infection. It is now recognized that the immune system monitors the metabolic state of tissues and responds by modulating metabolic function. The aim in this review is to summarize the literature that has defined a series of genotypic and phenotypic alterations in the regulatory host immune-metabolic signaling pathways in the local cecal microenvironment during the first 4 d following infection with Salmonella enterica serovar Enteritidis. Using chicken-specific kinomic immune-metabolism peptide arrays and quantitative real-time-PCR of cecal tissue during the early (4 to 48 h) and late stages (4 to 17 d) of a Salmonella infection in young broiler chickens, the local immunometabolic microenvironment has been ascertained. Distinct immune and metabolic pathways are altered between 2 to 4 d post-infection that dramatically changed the local immunometabolic environment. Thus, the tissue immunometabolic phenotype of the cecum plays a major role in the ability of the bacterium to establish a persistent cecal colonization. In general, our findings show that AMPK and mTOR are key players linking specific extracellular milieu and intracellular metabolism. Phenotypically, the early response (4 to 48 h) to Salmonella infection is pro-inflammatory, fueled by glycolysis and mTOR-mediated protein synthesis, whereas by the later phase (4 to 5 d), the local environment has undergone an immune-metabolic reprogramming to an anti-inflammatory state driven by AMPK-directed oxidative phosphorylation. Therefore, metabolism appears to provide a potential critical control point that can impact infection. Further understanding of metabolic control of immunity during infection should provide crucial information of the development of novel therapeutics based on metabolic modulators that enhance protection or inhibit infection. C1 [Kogut, Michael H.; Genovese, Kenneth J.; He, Haiqi] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX USA. [Arsenault, Ryan J.] Univ Delaware, Dept Anim & Food Sci, Newark, DE USA. RP Kogut, MH (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX USA. EM mike.kogut@ars.usda.gov NR 53 TC 2 Z9 2 U1 5 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2016 VL 95 IS 2 BP 345 EP 353 DI 10.3382/ps/pev349 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DF0WN UT WOS:000371060000013 PM 26706353 ER PT J AU Arsenault, RJ Genovese, KJ He, HQ Wu, HX Neish, AS Kogut, MH AF Arsenault, Ryan J. Genovese, Kenneth J. He, Haiqi Wu, Huixia Neish, Andrew S. Kogut, Michael H. TI Wild-type and mutant AvrA(-) Salmonella induce broadly similar immune pathways in the chicken ceca with key differences in signaling intermediates and inflammation SO POULTRY SCIENCE LA English DT Article DE kinome; Salmonella Typhimurium; peptide array; immunology; AvrA ID TYPHIMURIUM INFECTION; EFFECTOR PROTEIN; KINASE; DISEASE; INTERLEUKIN-10; CELLS; HOST; RISK; PATHOGENESIS; NEUTROPHIL AB Salmonella enterica serovar Typhimurium (ST) is a serious infectious disease throughout the world, and a major reservoir for Salmonella is chicken. Chicken infected with Salmonella do not develop clinical disease, this may be the result of important host interactions with key virulence proteins. To study this, we inoculated chicken with mutant Salmonella Typhimurium that lacked the virulence protein AvrA (AvrA(-)). AvrA is referred to as an avirulence factor, as it moderates the host immune response. The lack of the AvrA virulence gene in ST resulted in reduced weight gain, enhanced persistence and greater extraintestinal organ invasion in chickens, as compared to wild-type (WT) ST. Kinome analysis was performed on inoculated cecal tissue. The majority of the signal transduction pathways induced by AvrA(-) and WT ST were similar; however, we observed alterations in innate immune system signaling. In addition, a leukocyte migration pathway was altered by AvrA(-) ST that may allow greater gut barrier permeability and invasion by the mutant. Cytokine expression did not appear significantly altered at 7 d post-inoculation; at 14 d post-inoculation, there was an observed increase in the expression of anti-inflammatory IL-10 in the WT inoculated ceca. This study is the first to describe mutant AvrA(-) ST infection of chicken and provides further insight into the Salmonella responses observed in chicken relative to other species such as humans and cattle. C1 [Arsenault, Ryan J.] Univ Delaware, Dept Anim & Food Sci, 531 S Coll Ave, Newark, DE USA. [Genovese, Kenneth J.; He, Haiqi; Kogut, Michael H.] ARS, Southern Plains Agr Res Ctr, USDA, 2881 F&B Rd, College Stn, TX USA. [Wu, Huixia; Neish, Andrew S.] Emory Univ, Sch Med, Dept Pathol & Lab Med, Epithelial Pathobiol Unit, Atlanta, GA 30322 USA. RP Arsenault, RJ (reprint author), Univ Delaware, Dept Anim & Food Sci, 531 S Coll Ave, Newark, DE USA. EM rja@udel.edu NR 34 TC 1 Z9 1 U1 2 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2016 VL 95 IS 2 BP 354 EP 363 DI 10.3382/ps/pev344 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA DF0WN UT WOS:000371060000014 PM 26574031 ER PT J AU Swaggerty, CL McReynolds, JL Byrd, JA Pevzner, IY Duke, SE Genovese, KJ He, H Kogut, MH AF Swaggerty, C. L. McReynolds, J. L. Byrd, J. A. Pevzner, I. Y. Duke, S. E. Genovese, K. J. He, H. Kogut, M. H. TI Selection for pro-inflammatory mediators produces chickens more resistant to Clostridium perfringens-induced necrotic enteritis SO POULTRY SCIENCE LA English DT Article DE broiler; Clostridium perfringens; necrotic enteritis; pro-inflammatory; selection for resistance ID BROILER-CHICKENS; MODEL; COCCIDIOSIS; RESPONSES; POULTRY; HEALTH; LINES; TPEL AB We developed a novel selection method based on an inherently high and low phenotype of pro-inflammatory mediators and produced "high" and "low" line chickens. We have shown high line birds are more resistant to Salmonella enterica serovar Enteritidis and Eimeria tenella compared to the low line. Clostridium perfringens is the fourth leading cause of bacterial-induced foodborne illness, and is also an economically important poultry pathogen and known etiologic agent of necrotic enteritis (NE). The objective of this study was to determine if high line birds were also more resistant to NE than low line birds using an established model. Birds were reared in floor pens and challenges were conducted twice (high line = 25/trial, 50 birds total; low line = 26/trial, 52 birds total). Day-old chicks were provided a 55% wheat-corn-based un-medicated starter diet. A bursal disease vaccine was administered at 10x the recommended dose via the ocular route at 14-d-of-age. Birds were challenged daily for 3 d beginning at 16-d-of-age by oral gavage (3 mL) with 10(7) colony forming units (cfu) of C. perfringens/mL then necropsied at 21-d-of-age. All birds had sections of the intestine examined and scored for lesions while the first 10 necropsied also had gut content collected for C. perfringens enumeration. Chickens from the high line were more resistant to C. perfringens-induced NE pathology compared to the low line, as indicated by reduced lesion scores. Ninety percent of the high line birds had lesions of zero or one compared to 67% of the low line birds. Wilcoxon rank sum test showed significantly higher lesion scores in the low line birds compared to the high line (P < 0.0001). There were no differences in the C. perfringens recovered (P = 0.83). These data provide additional validation and support selection based on elevated levels of pro-inflammatory mediators produces chickens with increased resistance against foodborne and poultry pathogens. C1 [Swaggerty, C. L.; McReynolds, J. L.; Byrd, J. A.; Duke, S. E.; Genovese, K. J.; He, H.; Kogut, M. H.] ARS, USDA, SPARC, 2881 F&B Rd, College Stn, TX 77845 USA. [Pevzner, I. Y.; Duke, S. E.; Genovese, K. J.; He, H.] Cobb Vantress Inc, 4703 US Highway 412 East, Siloam Springs, AR 72761 USA. [McReynolds, J. L.] Elanco Anim Hlth, 2500 Innovat Way, Greenfield, IN 46140 USA. RP Swaggerty, CL (reprint author), ARS, USDA, SPARC, 2881 F&B Rd, College Stn, TX 77845 USA. EM christi.swaggerty@ars.usda.gov NR 35 TC 1 Z9 1 U1 6 U2 10 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2016 VL 95 IS 2 BP 370 EP 374 DI 10.3382/ps/pev348 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA DF0WN UT WOS:000371060000016 PM 26706357 ER PT J AU Nol, P Robbe-Austerman, S Rhyan, JC McCollum, MP Triantis, JM Beltran-Beck, B Salman, MD AF Nol, Pauline Robbe-Austerman, Suelee Rhyan, Jack C. McCollum, Matt P. Triantis, Joni M. Beltran-Beck, Beatriz Salman, Mo D. TI Determining the persistence of Mycobacterium bovis bacille Calmette-Guerin Danish in select tissues of orally vaccinated feral swine (Sus scrofa ssp.) SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Mycobacterium bovis; BCG; Feral swine; Vaccine; Bovine tuberculosis; Tissue persistence ID EUROPEAN WILD BOAR; RED DEER; IN-VIVO; TUBERCULOSIS; BCG; EPIDEMIOLOGY; TRANSMISSION; STRAINS; CATTLE AB Mycobacterium bovis bacille Calmette-Guerin (BCG) is being considered for vaccination of feral swine (Sus scrota ssp.). Since BCG is a live bacterium, evaluation of its safety and persistence in tissues is important. Fifteen feral swine received approximately 45 x 10(6) colony forming units of BCG Danish via oral bait. Four animals received bait without BCG. At 1, 3, 6, and 9 months post-vaccination, four vaccinates were euthanized. Non-vaccinates were euthanized at 9 months. Clinical signs were not noted in vaccinated pigs at any time. Tissues from all 20 pigs were culture-negative for mycobacteria. Based on our data, BCG is safe and appears not to persist in feral swine tissues after one month post-oral vaccination. However, further work must be performed at higher doses, and on a larger number of animals representing the target population, and further evaluation of persistence in tissues within the first month post-vaccination is needed. Published by Elsevier Ltd. C1 [Nol, Pauline; Rhyan, Jack C.; McCollum, Matt P.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Robbe-Austerman, Suelee] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Sci Technol & Anal Serv,Natl Vet Serv Labs, 1920 Dayton Ave, Ames, IA 50010 USA. [Triantis, Joni M.; Salman, Mo D.] Colorado State Univ, Anim Populat Hlth Inst, 1644 Campus Delivery, Ft Collins, CO 80523 USA. [Beltran-Beck, Beatriz] Inst Invest Recursos Cineget IREC, Sanidad & Biotecnol SaBio, Ronda Toledo S-N, Ciudad Real 13005, Spain. [Triantis, Joni M.] Colorado State Univ, Dept Environm Hlth Serv, 6021Campus Delivery, Ft Collins, CO 80523 USA. RP Nol, P (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM pauline.nol@aphis.usda.gov; suelee.robbe-austerman@aphis.usda.gov; jack.c.rhyan@aphis.usda.gov; matt.mccollum@aphis.usda.gov; joni.triantis@colostate.edu; beatriz.beltran@uclm.es; m.d.salman@colostate.edu OI Beltran-Beck, Beatriz/0000-0001-6689-5773 FU USDA APHIS Veterinary Services; MINECO [AGL2011-30,041]; EU FP7 grant WildTBVac FX This work was funded by USDA APHIS Veterinary Services and SaBio-IREC (Cuenta con Personal Perteneciente-Universidad de Castilla la Mancha-La Junta de Comunidades de Castilla La Mancha) is a contribution to Plan Nacional I + D + i research grant AGL2011-30,041 from MINECO and the EU FP7 grant WildTBVac. NR 19 TC 0 Z9 0 U1 4 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD FEB PY 2016 VL 104 BP 50 EP 52 DI 10.1016/j.rvsc.2015.11.007 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA DE6UM UT WOS:000370769100008 PM 26850536 ER PT J AU Rengaraj, D Truong, AD Lee, SH Lillehoj, HS Hong, YH AF Rengaraj, Deivendran Anh Duc Truong Lee, Sung-Hyen Lillehoj, Hyun S. Hong, Yeong Ho TI Expression analysis of cytosolic DNA-sensing pathway genes in the intestinal mucosal layer of necrotic enteritis-induced chicken SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Necrotic enteritis; Intestinal mucosal layer; CDS pathway; Chicken ID INNATE IMMUNE-RESPONSE; BROILER-CHICKENS; RNA-SEQ; CLOSTRIDIUM-PERFRINGENS; DIFFERENTIAL GENE; DAI DLM-1/ZBP1; HOST DNA; DISEASE; LINES; INFLAMMASOME AB Necrotic enteritis (NE) is a serious problem to the poultry farms, which report NE outbreaks more than once per year, as a result of the inappropriate use of antibiotics in the feed. The NE affected bird die rapidly as a result of various pathophysiological complications in the intestine and immune system. Also, several studies have reported that the genes exclusively related to intestine and immune functions are significantly altered in response to NE. In this study, NE was induced in two genetically disparate chicken lines that are resistant (line 6.3) and sensitive (line 7.2) to avian leukosis and Marek's disease. The intestinal mucosal layer was collected from NE-induced and control chickens, and subjected to RNA-sequencing analysis. The involvement of differentially expressed genes in the intestinal mucosal layer of line 6.3 and 7.2 with the immune system-related pathways was investigated. Among the identified immune system related pathways, a candidate pathway known as chicken cytosolic DNA-sensing pathway (CDS pathway) was selected for further investigation. RNA-sequencing and pathway analysis identified a total of 21 genes that were involved in CDS pathway and differentially expressed in the intestinal mucosal layer of lines 6.3 and 7.2. The expression of CDS pathway genes was further confirmed by real-time qPCR. In the results, a majority of the CDS pathway genes were significantly altered in the NE-induced intestinal mucosal layer from lines 6.3 and 7.2. In conclusion, our study indicate that NE seriously affects several genes involved in innate immune defense and foreign DNA sensing mechanisms in the chicken intestinal mucosal layer. Identifying the immune genes affected by NE could be an important evidence for the protective immune response to NE-causative pathogens. (C) 2015 Elsevier B.V. All rights reserved. C1 [Rengaraj, Deivendran; Anh Duc Truong; Hong, Yeong Ho] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 17546, Gyeonggi Do, South Korea. [Lee, Sung-Hyen] Natl Acad Agr Sci, Rural Dev Adm, Wanju Gun 55365, Jeollabuk Do, South Korea. [Lillehoj, Hyun S.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. RP Hong, YH (reprint author), Chung Ang Univ, Dept Anim Sci & Technol, Anseong 17546, Gyeonggi Do, South Korea. EM deivendran@cau.ac.kr; truonganhduc84@gmail.com; lshin@korea.kr; Hyun.Lillehoj@ars.usda.gov; yhong@cau.ac.kr OI Anh Duc, Truong/0000-0002-2472-8165 FU Golden Seed Project [PJ009925022016]; Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration, Republic of Korea [PJ01049004] FX This work was, in part, supported by the Golden Seed Project (No. PJ009925022016) and Cooperative Research Program for Agriculture Science & Technology Development (No. PJ01049004), Rural Development Administration, Republic of Korea. NR 44 TC 0 Z9 0 U1 3 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD FEB PY 2016 VL 170 BP 1 EP 12 DI 10.1016/j.vetimm.2015.12.010 PG 12 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DF2QT UT WOS:000371189600001 PM 26872625 ER PT J AU Boros, A Pankovics, P Adonyi, A Fenyvesi, H Day, JM Phan, TG Delwart, E Reuter, G AF Boros, Akos Pankovics, Peter Adonyi, Adam Fenyvesi, Hajnalka Day, J. Michael Tung Gia Phan Delwart, Eric Reuter, Gabor TI A diarrheic chicken simultaneously co-infected with multiple picornaviruses: Complete genome analysis of avian picornaviruses representing up to six genera SO VIROLOGY LA English DT Article DE Chicken; Bird; Poultry; Picornavirus; Metagenome; UTR; Diarrhea; Co-infection ID GENETIC-CHARACTERIZATION; GENUS AVIHEPATOVIRUS; MELEAGRIS-GALLOPAVO; RNA VIRUSES; PROTEIN; RECOMBINATION; SUPERFAMILY; SEQUENCE; ELEMENTS; DISTINCT AB In this study all currently known chicken picornaviruses including a novel one (chicken phacovirus 1, KT880670) were identified by viral metagenomic and RT-PCR methods from a single specimen of a diarrheic chicken suffering from a total of eight picornavirus co-infections, in Hungary. The complete genomes of six picornaviruses were determined and their genomic and phylogenetic characteristics and UTR RNA structural models analyzed in details. Picornaviruses belonged to genera Sicinivirus (the first complete genome), Gallivirus, Tremovirus, Avisivirus and "Orivirus" (two potential genotypes). In addition, the unassigned phacoviruses were also detected in multiple samples of chickens in the USA. Multiple co-infections promote and facilitate the recombination and evolution of picornaviruses and eventually could contribute to the severity of the diarrhea in chicken, in one of the most important food sources of humans. (C) 2015 Elsevier Inc. All rights reserved. C1 [Boros, Akos; Pankovics, Peter; Adonyi, Adam; Fenyvesi, Hajnalka; Reuter, Gabor] ANTSZ Reg Inst State Publ Hlth Serv, Natl Reference Lab Gastroenter Viruses, Reg Lab Virol, Szabadsag Ut 7, H-7623 Pecs, Hungary. [Boros, Akos; Pankovics, Peter; Adonyi, Adam; Fenyvesi, Hajnalka; Reuter, Gabor] Univ Pecs, Pecs, Hungary. [Day, J. Michael] ARS, USDA, US Natl Poultry Res Ctr, Southeast Poultry Res Lab, Athens, GA USA. [Tung Gia Phan; Delwart, Eric] Blood Syst Res Inst, San Francisco, CA USA. [Tung Gia Phan; Delwart, Eric] Univ Calif San Francisco, San Francisco, CA 94143 USA. RP Reuter, G (reprint author), ANTSZ Reg Inst State Publ Hlth Serv, Natl Reference Lab Gastroenter Viruses, Reg Lab Virol, Szabadsag Ut 7, H-7623 Pecs, Hungary. EM reuter.gabor@gmail.com RI Reuter, Gabor/I-7412-2013; OI Reuter, Gabor/0000-0002-5857-4934; Boros, Akos/0000-0001-5843-4360 FU Hungarian Scientific Research Fund [OTKA/NKFIH K111615]; NHLBI [R01-HL105770]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; European Union; State of Hungary; European Social Fund [TAMOP 4.2.4.A/ 2-11 /1-2012-0001]; Hungarian Society for Microbiology FX This work was financially supported by the grant from the Hungarian Scientific Research Fund (OTKA/NKFIH K111615) and by NHLBI R01-HL105770. B.A. was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences and the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TAMOP 4.2.4.A/ 2-11 /1-2012-0001'National Excellence Program'. A.A was supported by the Hungarian Society for Microbiology. NR 45 TC 0 Z9 0 U1 0 U2 7 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD FEB PY 2016 VL 489 BP 63 EP 74 DI 10.1016/j.virol.2015.12.002 PG 12 WC Virology SC Virology GA DE8NG UT WOS:000370892100007 PM 26707271 ER PT J AU Faburay, B Gaudreault, NN Liu, QF Davis, AS Shivanna, V Sunwoo, SY Lang, YK Morozov, I Ruder, M Drolet, B McVey, DS Ma, WJ Wilson, W Richt, JA AF Faburay, Bonto Gaudreault, Natasha N. Liu, Qinfang Davis, A. Sally Shivanna, Vinay Sunwoo, Sun Young Lang, Yuekun Morozov, Igor Ruder, Mark Drolet, Barbara McVey, D. Scott Ma, Wenjun Wilson, William Richt, Juergen A. TI Development of a sheep challenge model for Rift Valley fever SO VIROLOGY LA English DT Article DE Rift Valley fever; Sheep; Challenge model; Rift Valley fever virus; Strains; Viremia ID NORTH-AMERICAN MOSQUITOS; SAUDI-ARABIA; NSS PROTEIN; VIRUS; VACCINE; EXPRESSION; EPIDEMIC; ADULT; PATHOGENESIS; HEPATITIS AB Rift Valley fever (RVF) is a zoonotic disease that causes severe epizootics in ruminants, characterized by mass abortion and high mortality rates in younger animals. The development of a reliable challenge model is an important prerequisite for evaluation of existing and novel vaccines. A study aimed at comparing the pathogenesis of RVF virus infection in US sheep using two genetically different wild type strains of the virus (SA01-1322 and Kenya-128B-15) was performed. A group of sheep was inoculated with both strains and all infected sheep manifested early-onset viremia accompanied by a transient increase in temperatures. The Kenya-128B-15 strain manifested higher virulence compared to SA01-1322 by inducing more severe liver damage, and longer and higher viremia. Genome sequence analysis revealed sequence variations between the two isolates, which potentially could account for the observed phenotypic differences. We conclude that Kenya-128B-15 sheep infection represents a good and virulent challenge model for RVF. (C) 2015 Elsevier Inc. All rights reserved. C1 [Faburay, Bonto; Gaudreault, Natasha N.; Liu, Qinfang; Davis, A. Sally; Shivanna, Vinay; Sunwoo, Sun Young; Lang, Yuekun; Morozov, Igor; Ma, Wenjun; Richt, Juergen A.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Ruder, Mark; Drolet, Barbara; McVey, D. Scott; Wilson, William] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS USA. RP Richt, JA (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. EM jricht@vet.k-state.edu OI Sunwoo, Sun young/0000-0003-4688-641X; Shivanna, Vinay/0000-0002-4625-954X FU Grants of the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) [2010-ST061-AG0001]; USDA Agricultural Services Project [5430-050-005-00D]; Kansas State NBAF Transition Funds FX The authors wish to thank the staff of Kansas State University (KSU) Biosecurity Research Institute, the Comparative Medicine Group, and the staff of the Histopathology Unit at the Veterinary Diagnostic Laboratory. Additionally, we wish to thank Dane Jasperson, Lindsey Reister, Chester McDowell, Tammy Koopman, Haixia Liu, Maira Cotton-Caballero, Elizabeth Stietzle, Monica Gamez and Mal Hoover for technical assistance. This work was funded by Grants of the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Grant no. 2010-ST061-AG0001, USDA Agricultural Services Project 5430-050-005-00D and Kansas State NBAF Transition Funds. NR 51 TC 3 Z9 3 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD FEB PY 2016 VL 489 BP 128 EP 140 DI 10.1016/j.virol.2015.12.003 PG 13 WC Virology SC Virology GA DE8NG UT WOS:000370892100013 PM 26748334 ER PT J AU Smith, AMS Kolden, CA Paveglio, TB Cochrane, MA Bowman, DMJS Moritz, MA Kliskey, AD Alessa, L Hudak, AT Hoffman, CM Lutz, JA Queen, LP Goetz, SJ Higuera, PE Boschetti, L Flannigan, M Yedinak, KM Watts, AC Strand, EK van Wagtendonk, JW Anderson, JW Stocks, BJ Abatzoglou, JT AF Smith, Alistair M. S. Kolden, Crystal A. Paveglio, Travis B. Cochrane, Mark A. Bowman, David M. J. S. Moritz, Max A. Kliskey, Andrew D. Alessa, Lilian Hudak, Andrew T. Hoffman, Chad M. Lutz, James A. Queen, Lloyd P. Goetz, Scott J. Higuera, Philip E. Boschetti, Luigi Flannigan, Mike Yedinak, Kara M. Watts, Adam C. Strand, Eva K. van Wagtendonk, Jan W. Anderson, John W. Stocks, Brian J. Abatzoglou, John T. TI The Science of Firescapes: Achieving Fire-Resilient Communities SO BIOSCIENCE LA English DT Article DE wildland; fire; adaptation; mitigation; resilience ID WILDFIRE RISK-MITIGATION; WILDLAND-URBAN INTERFACE; CLIMATE-CHANGE; ADAPTIVE CAPACITY; WICKED PROBLEM; REGIME SHIFTS; FOREST; MANAGEMENT; VULNERABILITY; ECOSYSTEMS AB Wildland fire management has reached a crossroads. Current perspectives are not capable of answering interdisciplinary adaptation and mitigation challenges posed by increases in wildfire risk to human populations and the need to reintegrate fire as a vital landscape process. Fire science has been, and continues to be, performed in isolated "silos," including institutions (e.g., agencies versus universities), organizational structures (e.g., federal agency mandates versus local and state procedures for responding to fire), and research foci (e.g., physical science, natural science, and social science). These silos tend to promote research, management, and policy that focus only on targeted aspects of the "wicked" wildfire problem. In this article, we provide guiding principles to bridge diverse fire science efforts to advance an integrated agenda of wildfire research that can help overcome disciplinary silos and provide insight on how to build fire-resilient communities. C1 [Smith, Alistair M. S.; Kliskey, Andrew D.; Boschetti, Luigi; Yedinak, Kara M.; Strand, Eva K.] Univ Idaho, Coll Nat Resources, Moscow, ID 83843 USA. [Smith, Alistair M. S.] Univ Idaho, IFIRE, Moscow, ID 83843 USA. [Paveglio, Travis B.] Univ Idaho, Dept Nat Resources & Soc, Moscow, ID 83843 USA. [Kolden, Crystal A.] Univ Idaho, Coll Sci, Moscow, ID 83843 USA. [Cochrane, Mark A.] S Dakota State Univ, Geospatial Sci Ctr Excellence, Brookings, SD 57007 USA. [Bowman, David M. J. S.] Univ Tasmania, Hobart, Tas, Australia. [Moritz, Max A.] Univ Calif Berkeley, Coll Nat Resources, Berkeley, CA 94720 USA. [Alessa, Lilian] Univ Idaho, Ctr Resilient Commun, Alaska EPSCoR, Moscow, ID 83843 USA. [Alessa, Lilian] Univ Idaho, Int Arctic Res Center, Moscow, ID 83843 USA. [Kliskey, Andrew D.] USDA Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. [Hoffman, Chad M.] Colorado State Univ, Ft Collins, CO 80523 USA. [Hoffman, Chad M.] Western Forest Fire Res WESTFIRE, Ft Collins, CO USA. [Lutz, James A.] Utah State Univ, Logan, UT 84322 USA. [Queen, Lloyd P.; Higuera, Philip E.] Univ Montana, Missoula, MT 59812 USA. [Queen, Lloyd P.] FireCtr, Missoula, MT USA. [Goetz, Scott J.] Woods Hole Res Ctr, Falmouth, MA USA. [Goetz, Scott J.] Arct Boreal Vulnerabil Expt ABoVE, Falmouth, MA USA. [Flannigan, Mike] Univ Alberta, Edmonton, AB, Canada. [Flannigan, Mike] Western Partnership Western Fire Sci, Edmonton, AB, Canada. [Watts, Adam C.] Univ Nevada, Desert Res Inst, Reno, NV 89506 USA. [Anderson, John W.] Univ Idaho, Virtual Technol & Design, Moscow, ID 83843 USA. [Abatzoglou, John T.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. RP Smith, AMS (reprint author), Univ Idaho, Coll Nat Resources, Moscow, ID 83843 USA. EM alistair@uidaho.edu RI Bowman, David/A-2930-2011; Boschetti, Luigi/C-6198-2008; Higuera, Philip/B-1330-2010; OI Bowman, David/0000-0001-8075-124X; Boschetti, Luigi/0000-0001-6525-4413; Higuera, Philip/0000-0001-5396-9956; Abatzoglou, John/0000-0001-7599-9750 FU National Science Foundation [1520873, IIA-1301792, OIA-1208927]; National Aeronautics and Space Administration (NASA) [NNX11AO24G] FX Concepts for this manuscript were developed from a workshop on the Grand Fire Science Challenges hosted by the University of Idaho, in Moscow (16-18 April 2014). Partial funding for this research for CAK, JTA, LA, ADK, AMSS, JWA, and TBP was provided by the National Science Foundation under Hazards SEES award no. 1520873. AMSS was additionally funded by the National Aeronautics and Space Administration (NASA) under award no.NNX11AO24G. AMSS and JWA were partially funded by the National Science Foundation Idaho EPSCoR program under award no. IIA-1301792. LA and ADK were partially funded by the National Science Foundation Alaska EPSCoR program under award no. OIA-1208927. Special thanks to Rob Gazzard (the Forestry Commission England) and Ross Bradstock (the University of Wollongong) for reviewing the United Kingdom and Australian case studies, respectively. The case studies contain data produced by the governments of the United States, the United Kingdom, Australia, and Canada, and this data have not been produced in affiliation with, or with the endorsement of, these governments. NR 67 TC 11 Z9 11 U1 13 U2 32 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD FEB PY 2016 VL 66 IS 2 BP 130 EP 146 DI 10.1093/biosci/biv182 PG 17 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DD7PN UT WOS:000370116700008 ER PT J AU Allen, DK AF Allen, Doug K. TI Quantifying plant phenotypes with isotopic labeling & metabolic flux analysis SO CURRENT OPINION IN BIOTECHNOLOGY LA English DT Review ID HETEROTROPHIC ARABIDOPSIS CELLS; RESOLUTION MASS-SPECTROMETRY; GLOBAL FOOD DEMAND; MAIZE ROOT-TIPS; SULFUR METABOLISM; ENZYME-ACTIVITIES; SYSTEMS-ANALYSIS; OILSEED RAPE; AMINO-ACIDS; C-13 AB Analyses of metabolic flux using stable isotopes in plants have traditionally been restricted to tissues with presumed homogeneous cell populations and long metabolic steady states such as developing seeds, cell suspensions, or cultured roots and root tips. It is now possible to describe these and other metabolically more dynamic tissues such as leaves in greater detail using novel methods in mass spectrometry, isotope labeling strategies, and transient labeling-based flux analyses. Such studies are necessary for a systems level description of plant function that more closely represents biological reality, and provides insights into the genes that will need to be modified as natural resources become ever more limited and environments change. C1 [Allen, Doug K.] USDA ARS, Plant Genet Res Unit, 975 North Warson Rd, St Louis, MO 63132 USA. [Allen, Doug K.] Donald Danforth Plant Sci Ctr, 975 North Warson Rd, St Louis, MO 63132 USA. RP Allen, DK (reprint author), USDA ARS, Plant Genet Res Unit, 975 North Warson Rd, St Louis, MO 63132 USA.; Allen, DK (reprint author), Donald Danforth Plant Sci Ctr, 975 North Warson Rd, St Louis, MO 63132 USA. EM doug.allen@ars.usda.gov RI Allen, Doug/M-2836-2013 OI Allen, Doug/0000-0001-8599-8946 FU Agricultural Research Service of the United States Department of Agriculture FX This work was supported by the Agricultural Research Service of the United States Department of Agriculture. The author gratefully acknowledges Drs. Melvin Oliver and Elizabeth Kellogg for comments and edits that improved the review. NR 93 TC 2 Z9 2 U1 8 U2 28 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0958-1669 EI 1879-0429 J9 CURR OPIN BIOTECH JI Curr. Opin. Biotechnol. PD FEB PY 2016 VL 37 BP 45 EP 52 DI 10.1016/j.copbio.2015.10.002 PG 8 WC Biochemical Research Methods; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DE2LM UT WOS:000370458800007 PM 26613198 ER PT J AU Keena, MA AF Keena, M. A. TI Inheritance and World Variation in Thermal Requirements for Egg Hatch in Lymantria dispar (Lepidoptera: Erebidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE egg diapause; hybrid; Lymantria dispar; gypsy moth; inheritance ID GYPSY-MOTH; TEMPERATURE; DIAPAUSE; DISPERSAL; EMERGENCE; PHENOLOGY; STRAIN AB Mode of inheritance of hatch traits in Lymantria dispar L. was determined by crossing populations nearly fixed for the phenotypic extremes. The nondiapausing phenotype was inherited via a single recessive gene and the phenotype with reduced low temperature exposure requirements before hatch was inherited via a single dominant gene. There was no evidence for sex-linkage or cytoplasmic effects with either gene. Eggs from 43 geographic populations were evaluated for hatch characteristics after being held for 60d at 5 degrees C followed by incubation at 25 degrees C. There was considerable variation both within and among the populations in the proportion able to hatch, time to first hatch, and average time to hatch. Egg masses with reduced requirement for low temperatures before the eggs were ready to hatch were present in all subspecies of L. dispar and the phenotype was not fixed in most populations. The populations clustered into three distinct groups, and climatic variables were found to be rough predictors of those groups. Variation in hatch phenotypes between populations is likely an adaptation to local climate and within a population provides a bet-hedging strategy to ensure that at least some hatch synchronizes with host leaf-out. Continued vigilance to prevent movement of populations both within and between countries is warranted, because some of the alleles that confer nondiapause or reduced low temperature requirements before egg hatch are not present in all populations and their introduction would increase variation in egg hatch within a population. C1 [Keena, M. A.] US Forest Serv, USDA, No Res Stn, Hamden, CT 06514 USA. RP Keena, MA (reprint author), US Forest Serv, USDA, No Res Stn, Hamden, CT 06514 USA. EM mkeena@fs.fed.us NR 39 TC 0 Z9 0 U1 8 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 1 EP 10 DI 10.1093/ee/nvv163 PG 10 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900001 PM 26510608 ER PT J AU Pfammatter, JA Coyle, DR Gandhi, KJK Hernandez, N Hofstetter, RW Moser, JC Raffa, KF AF Pfammatter, Jesse A. Coyle, David R. Gandhi, Kamal J. K. Hernandez, Natalie Hofstetter, Richard W. Moser, John C. Raffa, Kenneth F. TI Structure of Phoretic Mite Assemblages Across Subcortical Beetle Species at a Regional Scale SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE commensalism; Dendroctonus; Ips; phoresy; Pinus ID SOUTHERN PINE-BEETLE; BARK BEETLES; COLEOPTERA; SCOLYTIDAE; DIVERSITY; ASSOCIATIONS; RAREFACTION; DYNAMICS; FUNGI AB Mites associated with subcortical beetles feed and reproduce within habitats transformed by tree-killing herbivores. Mites lack the ability to independently disperse among these habitats, and thus have evolved characteristics that facilitate using insects as transport between resources. Studies on associations between mites and beetles have historically been beetle-centric, where an assemblage of mite species is characterized on a single beetle species. However, available evidence suggests there may be substantial overlap among mite species on various species of beetles utilizing similar host trees. We assessed the mite communities of multiple beetle species attracted to baited funnel traps in Pinus stands in southern Wisconsin, northern Arizona, and northern Georgia to better characterize mite dispersal and the formation of mite-beetle phoretic associations at multiple scales. We identified approximately 21 mite species totaling 10,575 individuals on 36 beetle species totaling 983 beetles. Of the mites collected, 97% were represented by eight species. Many species of mites were common across beetle species, likely owing to these beetles' common association with trees in the genus Pinus. Most mite species were found on at least three beetle species. Histiostoma spp., Iponemus confusus Lindquist, Histiogaster arborsignis Woodring and Trichouropoda australis Hirschmann were each found on at least seven species of beetles. While beetles had largely similar mite membership, the abundances of individual mite species were highly variable among beetle species within each sampling region. Phoretic mite communities also varied within beetle species between regions, notably for Ips pini (Say) and Ips grandicollis (Eichhoff). C1 [Pfammatter, Jesse A.; Hernandez, Natalie; Raffa, Kenneth F.] Univ Wisconsin, Dept Entomol, 345 Russell Labs, Madison, WI 53706 USA. [Coyle, David R.; Gandhi, Kamal J. K.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, 180 Green St, Athens, GA 30602 USA. [Hofstetter, Richard W.] No Arizona Univ, Sch Forestry, 200 E Pine Knoll Dr, Flagstaff, AZ 86011 USA. [Moser, John C.] US Forest Serv, USDA, Washington, DC USA. RP Pfammatter, JA (reprint author), Univ Wisconsin, Dept Entomol, 345 Russell Labs, Madison, WI 53706 USA. EM pfammatter@wisc.edu; dcoyle@warnell.uga.edu; kgandhi@warnell.uga.edu; nhernandez@wisc.edu; rich.hofstetter@nau.edu FU McIntire-Stennis [WIS01430]; University of Wisconsin-Madison College of Agricultural and Life Sciences FX John Moser not only coauthored this paper but also provided the foundation on which it was built through his decades of groundbreaking work on phoretic mites. He passed the day after its acceptance. Kurt Steele (Oconee NF, USFS) and William Lott (D. B. Warnell School of Forestry and Natural Resources, UGA) helped select sites in Georgia. Nicholas Aflitto (Northern Arizona University); Courtney Brissey and Kris Smoot (D. B. Warnell School of Forestry and Natural Resources, UGA); and Nichole Boyea, Lukas Brown, Stephanie Jagemann, Kareem Malas, and Thomas Olson (University of Wisconsin-Madison) assisted with field operations, sample preparation, and beetle identification. Claudio Gratton, Tony Ives, John Orrock, and Dan Mahr (University of Wisconsin-Madison) provided helpful reviews. This work was supported by McIntire-Stennis WIS01430 and the University of Wisconsin-Madison College of Agricultural and Life Sciences. Comments by three anonymous reviewers improved our manuscript. NR 52 TC 0 Z9 0 U1 0 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 53 EP 65 DI 10.1093/ee/nvv150 PG 13 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900008 PM 26496952 ER PT J AU Limbu, S Cassidy, K Keena, M Tobin, P Hoover, K AF Limbu, Samita Cassidy, Katie Keena, Melody Tobin, Patrick Hoover, Kelli TI Host Range Specificity of Scymnus camptodromus (Coleoptera: Coccinellidae), A Predator of Hemlock Woolly Adelgid (Hemiptera: Adelgidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE host range; Scymnus camptodromus; biological control; nontarget effect; invasive species ID BIOLOGICAL-CONTROL AGENT; LARICOBIUS-NIGRINUS; EASTERN HEMLOCK; SASAJISCYMNUS-TSUGAE; NORTH-AMERICA; UNITED-STATES; HOMOPTERA; DERODONTIDAE; ESTABLISHMENT; TEMPERATURE AB Scymnus (Neopullus) camptodromus Yu and Liu (Coleoptera: Coccinellidae) was brought to the United States from China as a potential biological control agent for hemlock woolly adelgid (Adelges tsugae Annand) (Hemiptera: Adelgidae). Scymnus camptodromus phenology is closely synchronized with that of A. tsugae and has several characteristics of a promising biological control agent. As a prerequisite to field release, S. camptodromus was evaluated for potential nontarget impacts. In host range studies, the predator was given the choice of sympatric adelgid and nonadelgid prey items. Nontarget testing showed that S. camptodromus will feed to some degree on other adelgid species, but highly prefers A. tsugae. We also evaluated larval development of S. camptodromus on pine bark adelgid (Pineus strobi (Hartig)) (Hemiptera: Adelgidae) and larch adelgid (Adelges laricis Vallot) (Hemiptera: Adelgidae); a small proportion of predator larvae was able to develop to adulthood on P. strobi or A. laricis alone. Scymnus camptodromus showed no interest in feeding on woolly alder aphid (Paraprociphilus tessellatus Fitch) (Hemiptera: Aphididae) or woolly apple aphid (Eriosoma lanigerum (Hausmann)) (Hemiptera: Aphididae), and minimal interest in cotton aphid (Aphis gossypii Glover) (Hemiptera: Aphididae) in choice and no-choice experiments. Scymnus camptodromus females did not oviposit on any host material other than A. tsugae-infested hemlock. Under the circumstances of the study, S. camptodromus appears to be a specific predator of A. tsugae, with minimal risk to nontarget species. Although the predator can develop on P. strobi, the likelihood that S. camptodromus would oviposit on pine hosts of this adelgid is small. C1 [Limbu, Samita; Cassidy, Katie; Hoover, Kelli] Penn State Univ, Dept Entomol, 501 ASI Bldg, University Pk, PA 16802 USA. [Limbu, Samita; Cassidy, Katie; Hoover, Kelli] Penn State Univ, Ctr Chem Ecol, 501 ASI Bldg, University Pk, PA 16802 USA. [Keena, Melody] US Forest Serv, USDA, No Res Stn, 51 Mill Pond, Hamden, CT 06514 USA. [Tobin, Patrick] Univ Washington, Sch Environm & Forest Sci, 123 Anderson Hall, Seattle, WA 98195 USA. RP Hoover, K (reprint author), Penn State Univ, Dept Entomol, 501 ASI Bldg, University Pk, PA 16802 USA.; Hoover, K (reprint author), Penn State Univ, Ctr Chem Ecol, 501 ASI Bldg, University Pk, PA 16802 USA. EM szl175@psu.edu; kem5052@gmail.com; mkeena@fs.fed.us; pctobin@uw.edu; kxh25@psu.edu FU USDA Forest Service Northeastern Area (NA) State and Private Forestry (S&PF) Technology Development competitive, Hemlock Woolly Adelgid Initiative funding [13-CA-11422224-060, 09-DG-1142004-330] FX We thank V. Sanchez and A. Vandel for assistance in the laboratory and Gary Thompson's lab for providing aphids for this study. Funding was provided in part by two USDA Forest Service Northeastern Area (NA) State and Private Forestry (S&PF) Technology Development competitive grants to K. H.: 13-CA-11422224-060-part of the Joint Hemlock Woolly Adelgid Initiative funding-and 09-DG-1142004-330 with allocation through PA DCNR to Improve Management of Hemlock Woolly Adelgid. NR 39 TC 0 Z9 0 U1 3 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 94 EP 100 DI 10.1093/ee/nvv174 PG 7 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900012 PM 26612409 ER PT J AU Hogg, BN Smith, L Daane, KM AF Hogg, Brian N. Smith, Lincoln Daane, Kent M. TI Impacts of the Adventive Psyllid Arytainilla spartiophila (Hemiptera: Psyllidae) on Growth of the Invasive Weed Cytisus scoparius Under Controlled and Field Conditions in California SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE agent efficacy; Arytainilla; Cytisus; Psyllidae; weed control ID BOREIOGLYCASPIS-MELALEUCAE HEMIPTERA; BIOLOGICAL-CONTROL AGENT; SAROTHAMNUS-SCOPARIUS; INTRODUCED INSECTS; L WIMMER; BROOM; HERBIVORY; EFFICACY; QUINQUENERVIA; PLANTS AB The postrelease impact of weed biological control agents on their target weeds is rarely assessed. This study focuses on the impacts of the univoltine broom psyllid Arytainilla spartiophila Forster on the growth of its target weed, the invasive shrub Scotch broom, Cytisus scoparius (L.) Link (Fabaceae), in California. Arytainilla spartiophila is an adventive species that has been present in North America for several decades. In a greenhouse experiment, plant growth as measured by both height and total stem length (height+length of all branches) was reduced on plants that received psyllids, and psyllid densities were negatively correlated to proportional change in plant height. There were no effects on number of leaves, however. Furthermore, the psyllid had no statistically detectable impacts on plant growth parameters in a field experiment, underscoring how results obtained under controlled conditions may not fully predict the performance of biological control agents in the field. The high psyllid mortality that occurred at higher psyllid densities in both the greenhouse and the field suggests that the impacts of A. spartiophila may be moderated by intraspecific competition. C1 [Hogg, Brian N.; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, 137 Mulford Hall, Berkeley, CA 94720 USA. [Hogg, Brian N.; Smith, Lincoln] USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA USA. RP Hogg, BN (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, 137 Mulford Hall, Berkeley, CA 94720 USA.; Hogg, BN (reprint author), USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA USA. EM hoggbrian@yahoo.com; link.smith@ars.usda.gov; kdaane@ucanr.edu NR 56 TC 0 Z9 0 U1 5 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 109 EP 116 DI 10.1093/ee/nvv179 PG 8 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900014 PM 26628515 ER PT J AU West, DR Briggs, JS Jacobi, WR Negron, JF AF West, Daniel R. Briggs, Jennifer S. Jacobi, William R. Negron, Jose F. TI Mountain Pine Beetle Host Selection Between Lodgepole and Ponderosa Pines in the Southern Rocky Mountains SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Dendroctonus ponderosae; Pinus contorta; Pinus ponderosa; host selection; Hopkins' Host Selection Principle ID BARK BEETLES; DENDROCTONUS-PONDEROSAE; RANGE EXPANSION; HOPKINS COLEOPTERA; BOREAL FOREST; SCOLYTIDAE; TEMPERATURE; MORTALITY; METAMORPHOSIS; CURCULIONIDAE AB Recent evidence of range expansion and host transition by mountain pine beetle (Dendroctonus ponderosae Hopkins; MPB) has suggested that MPB may not primarily breed in their natal host, but will switch hosts to an alternate tree species. As MPB populations expanded in lodgepole pine forests in the southern Rocky Mountains, we investigated the potential for movement into adjacent ponderosa pine forests. We conducted field and laboratory experiments to evaluate four aspects of MPB population dynamics and host selection behavior in the two hosts: emergence timing, sex ratios, host choice, and reproductive success. We found that peak MPB emergence from both hosts occurred simultaneously between late July and early August, and the sex ratio of emerging beetles did not differ between hosts. In two direct tests of MPB host selection, we identified a strong preference by MPB for ponderosa versus lodgepole pine. At field sites, we captured naturally emerging beetles from both natal hosts in choice arenas containing logs of both species. In the laboratory, we offered sections of bark and phloem from both species to individual insects in bioassays. In both tests, insects infested ponderosa over lodgepole pine at a ratio of almost 2:1, regardless of natal host species. Reproductive success (offspring/female) was similar in colonized logs of both hosts. Overall, our findings suggest that MPB may exhibit equally high rates of infestation and fecundity in an alternate host under favorable conditions. C1 [West, Daniel R.; Jacobi, William R.] Colorado State Univ, Colorado State Forest Serv, 5060 Campus Delivery, Ft Collins, CO 80523 USA. [Briggs, Jennifer S.] US Geol Survey, Geosci & Environm Change Sci Ctr, Denver Fed Ctr, Box 25046,MS 980,Bldg 25, Denver, CO 80225 USA. [Negron, Jose F.] US Forest Serv, Rocky Mt Res Stn, 240 West Prospect, Ft Collins, CO 80525 USA. RP West, DR (reprint author), Colorado State Univ, Colorado State Forest Serv, 5060 Campus Delivery, Ft Collins, CO 80523 USA. EM dan.west@colostate.edu; jsbriggs@usgs.gov; William.Jacobi@colostate.edu; jnegron@fs.fed.us FU US Geological Survey Land Change Science program of the Climate and Land Use Mission Area; Colorado State Forest Service; Colorado Agricultural Experiment Station FX This work was funded in part by the US Geological Survey Land Change Science program of the Climate and Land Use Mission Area; Colorado State Forest Service; and the Colorado Agricultural Experiment Station. We thank the US Forest Service, Arapaho-Roosevelt NF for logistic support and access to sampling sites. We thank James zumBrunnen and the Franklin A. Graybill Statistical Laboratory, Colorado State University, for statistical consultation and guidance. We thank Sheryl Costello for conversations and input regarding field-based study design, and Craig Allen for general guidance. We thank the US Forest Service, Rocky Mountain Research Station for laboratory space for the rearing study. We thank Ken Raffa for collaborating on the laboratory bioassay design. We appreciate review comments provided by Jeff Witcosky and Dave Leatherman on a previous draft. We thank the field technicians Barbara West, Kevin Miller, Zach Wehr, Jake Thomsen, Paul Cigan, Tim Mapoles, Patrick Flynn, Brison Bishop, Justin Pomeranz, Eric Eden, and Peter Pavlowich for their back-breaking hauling of logs through the forests, tireless data collection, and meticulous record-keeping. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 71 TC 1 Z9 1 U1 8 U2 21 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 127 EP 141 DI 10.1093/ee/nvv167 PG 15 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900016 PM 26546596 ER PT J AU Ampt, EA Bush, DS Siegel, JP Berenbaum, MR AF Ampt, Eline A. Bush, Daniel S. Siegel, Joel P. Berenbaum, May R. TI Larval Preference and Performance of Amyelois transitella (Navel Orangeworm, Lepidoptera: Pyralidae) in Relation to the Fungus Aspergillus flavus SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE navel orangeworm; Aspergillus flavus; preference; performance; mutualism ID FATTY-ACID-COMPOSITION; LOBESIA-BOTRANA; AFLATOXIN CONTAMINATION; BOTRYTIS-CINEREA; WATER ACTIVITY; GROUND ALMOND; EGG TRAPS; PISTACHIO; BEHAVIOR; MOTH AB The navel orangeworm, Amyelois transitella (Walker), is a polyphagous pest of California nut crops and is responsible for extensive losses in the United States. It directly damages crops by feeding and contaminating nuts with frass and webbing and vectors saprophytic fungi that infect crops. The navel orangeworm is commonly associated with Aspergillus species, including the toxigenic Aspergillus flavus, which causes crop loss by producing carcinogens, including aflatoxin B1. This lepidopteran-fungus association is the most economically serious pest complex in Central Valley orchards, and evidence indicates that this relationship is mutualistic. We assessed preference and performance of navel orangeworm larvae associated with A. flavus in behavioral bioassays in which neonates were allowed to orient within arenas to media with or without fungal tissue, and performance bioassays in which larvae were reared with and without A. flavus on potato dextrose agar (PDA) and a semidefined almond PDA diet to evaluate effects on development and pupal weight. Navel orangeworm larvae were attracted to A. flavus and developed faster in its presence, indicating a nutritional benefit to the caterpillars. Larvae reached pupation similar to 33% faster on diet containing A. flavus, and pupal weights were similar to 18% higher for males and similar to 13% higher for females on this diet. Our findings indicate that A. flavus plays an important role in larval orientation and development on infected hosts. The preference-performance relationship between navel orangeworms and Aspergillus flavus is consistent with a facultative mutualism that has broad implications for pest management efforts and basic understanding of Lepidoptera-plant interactions. C1 [Ampt, Eline A.; Bush, Daniel S.; Berenbaum, May R.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Siegel, Joel P.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Bush, DS (reprint author), Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. EM ampt2@illinois.edu; dsbush2@illinois.edu; joel.siegel@ars.usda.gov; maybe@illinois.edu FU Almond Board of California; California Pistachio Research Board FX Aspergillus flavus AF36 was provided by Themis Michailides of the University of California, Davis. We thank Mark Demkovich and Catherine Dana for advice and assistance with navel orangeworm methods and Andrew Miller and Daniel Raudabaugh for assistance with fungal techniques. Funding for this project was provided by the Almond Board of California and the California Pistachio Research Board. USDA-ARS is an equal opportunity provider and employer. NR 37 TC 1 Z9 1 U1 6 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 155 EP 162 DI 10.1093/ee/nvv160 PG 8 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900019 PM 26491042 ER PT J AU Yocum, GD Rinehart, JP Yocum, IS Kemp, WP Greenlee, KJ AF Yocum, George D. Rinehart, Joseph P. Yocum, Ian S. Kemp, William P. Greenlee, Kendra J. TI Thermoperiodism Synchronizes Emergence in the Alfalfa Leafcutting Bee (Hymenoptera: Megachilidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE alfalfa leafcutting bee; fluctuating thermal regime; insect storage ID CIRCADIAN ECLOSION RHYTHM; ROTUNDATA HYMENOPTERA; ADULT ECLOSION; SARCOPHAGA-CRASSIPALPIS; FLESH FLY; DELIA-ANTIQUA; SOLITARY BEE; ONION FLY; TEMPERATURE; SURVIVAL AB Alfalfa seed production in the northwestern United States and western Canada is heavily dependent upon the pollinating services of Megachile rotundata (F.) (Hymenoptera: Megachilidae). M. rotundata females nest in cavities either naturally occurring or in artificial nesting blocks. Because of the physical nature of the nest, M. rotundata brood may have limited to no exposure to photoperiodic cues in order to regulate important circadian functions. Therefore, various thermoperiod regimes were used to characterize the possible role of thermoperiodism in synchronizing M. rotundata adult emergence. Adult emergence was monitored using a microprocessor- controlled event logger. Incubating bees under constant 29 degrees C and darkness resulted in an arhythmic adult emergence pattern. Exposing developing M. rotundata to a thermoperiod synchronized emergence to the beginning of the thermophase and decreased the total number of days required for all adults to emerge. The amplitude of the thermoperiod regulated the timing of peak emergence in relationship to the increase in temperature. A thermoperiod amplitude of only 2 degrees C was sufficient to synchronize peak adult emergence to take place during the rise in temperature. Increasing the amplitude of the thermoperiod to 4 or 8 degrees C caused a positively correlated shift in peak emergence to later in the thermophase. Brood stored under constant 29 degrees C and darkness for different durations (May or June early in the growing season or July or August late in the growing season) or under a fluctuating thermal regime (base temperature of 6 degrees C and daily 1-h pulse of 20 degrees C until September or November) maintained their capacity for entraining emergence timing by thermoperiodism. C1 [Yocum, George D.; Rinehart, Joseph P.; Yocum, Ian S.; Kemp, William P.] USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Greenlee, Kendra J.] N Dakota State Univ, Dept Biol Sci, POB 6050,Dept 2715, Fargo, ND 58108 USA. RP Yocum, GD (reprint author), USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM george.yocum@ars.usda.gov; Joseph.Rinehart@ars.usda.gov; ian.yocum@ars.usda.gov; william.kemp@ars.usda.gov; kendra.greenlee@ndsu.edu NR 49 TC 1 Z9 1 U1 4 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2016 VL 45 IS 1 BP 245 EP 251 DI 10.1093/ee/nvv152 PG 7 WC Entomology SC Entomology GA DD9UT UT WOS:000370271900031 PM 26385932 ER PT J AU Erickson, MJ Charney, JJ Colle, BA AF Erickson, Michael J. Charney, Joseph J. Colle, Brian A. TI Development of a Fire Weather Index Using Meteorological Observations within the Northeast United States SO JOURNAL OF APPLIED METEOROLOGY AND CLIMATOLOGY LA English DT Article DE Wildfires; Statistical techniques; Regression analysis; Forest fires; Surface observations; Statistical forecasting ID CLIMATOLOGY; FOREST AB A fire weather index (FWI) is developed using wildfire occurrence data and Automated Surface Observing System weather observations within a subregion of the northeastern United States (NEUS) from 1999 to 2008. Average values of several meteorological variables, including near-surface temperature, relative humidity, dewpoint, wind speed, and cumulative daily precipitation, are compared on observed wildfire days with their climatological average ("climatology") using a bootstrap resampling approach. Average daily minimum relative humidity is significantly lower than climatology on wildfire occurrence days, and average daily maximum temperature and average daily maximum wind speed are slightly higher on wildfire occurrence days. Using the potentially important weather variables (relative humidity, temperature, and wind speed) as inputs, different formulations of a binomial logistic regression model are tested to assess the potential of these atmospheric variables for diagnosing the probability of wildfire occurrence. The FWI is defined using probabilistic output from the preferred binomial logistic regression configuration. Relative humidity and temperature are the only significant predictors in the binomial logistic regression. The binomial logistic regression model is reliable and has more probabilistic skill than climatology using an independent verification dataset. Using the binomial logistic regression output probabilities, an FWI is developed ranging from 0 (minimum potential) to 3 (high potential) and is verified independently for two separate subdomains within the NEUS. The climatology of the FWI reproduces observed fire occurrence probabilities between 1999 and 2008 over a subdomain of the NEUS. C1 [Erickson, Michael J.; Colle, Brian A.] SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA. [Charney, Joseph J.] US Forest Serv, USDA, No Res Stn, E Lansing, MI USA. RP Erickson, MJ (reprint author), SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA. EM mjaerickson@gmail.com FU Stony Brook University [08-JV-11242306-093]; U.S. Forest Service [08-JV-11242306-093] FX This work was supported by a research joint venture agreement between Stony Brook University and the U.S. Forest Service (08-JV-11242306-093). We thank the reviewers for their constructive comments that helped improve this manuscript. NR 19 TC 0 Z9 1 U1 3 U2 7 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1558-8424 EI 1558-8432 J9 J APPL METEOROL CLIM JI J. Appl. Meteorol. Climatol. PD FEB PY 2016 VL 55 IS 2 BP 389 EP 402 DI 10.1175/JAMC-D-15-0046.1 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DE1WZ UT WOS:000370418500002 ER PT J AU Andow, DA Pueppke, SG Schaafsma, AW Gassmann, AJ Sappington, TW Meinkei, LJ Mitche, PD Hurley, TM Hellmich, RL Porterl, RP AF Andow, David A. Pueppke, Steven G. Schaafsma, Arthur W. Gassmann, Aaron J. Sappington, Thomas W. Meinkei, Lance J. Mitche, Paul D. Hurley, Terrance M. Hellmich, Richard L. Porterl, R. Pat TI Early Detection and Mitigation of Resistance to Bt Maize by Western Corn Rootworm (Coleoptera: Chrysomelidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bt resistance; Diabrotica virgifera virgifera; insect resistance management; integrated pest management; western corn rootworm ID DIABROTICA-VIRGIFERA-VIRGIFERA; FIELD-EVOLVED RESISTANCE; FRUGIPERDA LEPIDOPTERA-NOCTUIDAE; BACILLUS-THURINGIENSIS TOXIN; INTEGRATED PEST-MANAGEMENT; EVENT DAS-59122-7 MAIZE; INSECT RESISTANCE; TRANSGENIC-MAIZE; FITNESS COSTS; LARVAL INJURY AB Transgenic Bt maize that produces less than a high-dose has been widely adopted and presents considerable insect resistance management (IRM) challenges. Western corn rootworm, Diabrotica virgifera virgifera LeConte, has rapidly evolved resistance to Bt maize in the field, leading to local loss of efficacy for some corn rootworm Bt maize events. Documenting and responding to this resistance has been complicated by a lack of rapid diagnostic bioassays and by regulatory triggers that hinder timely and effective management responses. These failures are of great concern to the scientific and agricultural community. Specific challenges posed by western corn rootworm resistance to Bt maize, and more general concerns around Bt crops that produce less than a high-dose of Bt toxin, have caused uncertainty around current IRM protocols. More than 15 years of experience with IRM has shown that high-dose and refuge-based IRM is not applicable to Bt crops that produce less than a high-dose. Adaptive IRM approaches and pro-active, integrated IRM-pest management strategies are needed and should be in place before release of new technologies that produce less than a high-dose. We suggest changes in IRM strategies to preserve the utility of corn rootworm Bt maize by 1) targeting local resistance management earlier in the sequence of responses to resistance and 2) developing area-wide criteria to address widespread economic losses. We also favor consideration of policies and programs to counteract economic forces that are contributing to rapid resistance evolution. C1 [Andow, David A.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Pueppke, Steven G.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Schaafsma, Arthur W.] Univ Guelph, Ridgetown Campus, Ridgetown, ON N0P 2C0, Canada. [Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Sappington, Thomas W.; Hellmich, Richard L.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Meinkei, Lance J.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Mitche, Paul D.] Univ Wisconsin, Dept Agr & Appl Econ, Madison, WI 53706 USA. [Hurley, Terrance M.] Univ Minnesota, Dept Appl Econ, St Paul, MN 55108 USA. [Porterl, R. Pat] Texas A&M Agrilife Res & Extens Ctr, Lubbock, TX 79403 USA. RP Andow, DA (reprint author), Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. EM dandow@umn.edu; pueppke@anr.msu.edu; aschaafs@uoguelph.ca; aaronjg@iastate.edu; Tom.Sappington@ars.usda.gov; LMEINKE1@unl.edu; pdmitchell@wisc.edu; tmh@umn.edu; Richard.Hellmich@ars.usda.gov; pporter@ag.tamu.edu OI Hurley, Terrance/0000-0003-2135-7570 FU USDA Regional Research Committee [NC-205]; USDA Multistate Research Coordinating Committee [NCCC-46]; Agricultural Experiment Stations in Minnesota, Iowa, Illinois; Nebraska FX We thank Eileen Cullen, Mike Gray, Torn Hunt, Chuck Mason, Ken Ostlie, and Blair Siegfried for discussions early in the development of tins paper. This work was supported by the USDA Regional Research Committee NC-205 and the USDA Multistate Research Coordinating Committee NCCC-46, and with funds from Agricultural Experiment Stations in Minnesota, Iowa, Illinois, and Nebraska. Mention of a proprietary product does not constitute an endorsement or a recommendation by the above Universities or USDA for its use. NR 118 TC 9 Z9 9 U1 7 U2 40 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 1 EP 12 DI 10.1093/jee/tov238 PG 12 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800001 PM 26362989 ER PT J AU Sim, SB Yoneishi, NM Brill, E Geib, SM Follett, PA AF Sim, Sheina B. Yoneishi, Nicole M. Brill, Eva Geib, Scott M. Follett, Peter A. TI Molecular Markers Detect Cryptic Predation on Coffee Berry Borer (Coleoptera: Curculionidae) by Silvanid and Laemophloeid Flat Bark Beetles (Coleoptera: Silvanidae, Laemophloeidae) in Coffee Beans SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE coffee berry borer; predator-prey interaction; biocontrol; silvanid flat bark beetle; laemophloeid flat bark beetle ID HYPOTHENEMUS-HAMPEI COLEOPTERA; SCOLYTIDAE; BIOLOGY AB The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is a serious pest of coffee worldwide. It was first detected in Hawai'i in 2010. Two predatory beetles, Cathartus quadricollis (Coleoptera: Silvanidae) and Leptophloeus sp. (Coleoptera: Laemophloeidae), have been observed in H. hampei-infested coffee. Under laboratory conditions, colony-reared C. quadricollis and Leptophloeus sp. prey upon all life stages of H. hampei. However, the H. hampei life cycle occurs almost exclusively within a coffee bean obscured from direct observation. Thus, it is unknown if C. quadricollis and Leptophloeus sp. consume H. hampei as prey in the wild. To demonstrate predation of H. hampei by C. quadricollis and Leptophloeus sp., a molecular assay was developed utilizing species-specific primers targeting short regions of the mitochondrial COI gene to determine species presence. Using these primers, wild C. quadricollis and Leptophloeus sp. were collected and screened for the presence of H. hampei DNA using PCR. Analysis of collections from five coffee farms revealed predation of C. quadricollis and Leptophloeus sp. on H. hampei. Further laboratory testing showed that H. hampei DNA could be detected in predators for as long as 48 h after feeding, indicating the farm-caught predators had preyed on H. hampei within 2 d of sampling. This study demonstrates the utility of molecular markers for the study of the ecology of predators and prey with cryptic behavior, and suggests C. quadricollis and Leptophloeus sp. might be useful biocontrol agents against H. hampei. C1 [Sim, Sheina B.; Yoneishi, Nicole M.; Brill, Eva; Geib, Scott M.; Follett, Peter A.] ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Sim, Sheina B.] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Plant & Environm Protect Sci, 3050 Maile Way, Honolulu, HI 96822 USA. RP Follett, PA (reprint author), ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM ssim8@hawaii.edu; nicole.yoneishi@ars.usda.gov; eva.brill@ars.usda.gov; scott.geib@ars.usda.gov; peter.follett@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service (USDA-ARS); Hawai'i Department of Agriculture (HDOA) [CBBRD14]; University of Hawai'i College of Tropical Agriculture and Human Resources (UH CTAHR) [2040-21000-015-14]; USDA Farm Bill Project [3.025] FX We would like to acknowledge Glenn Asians, Allison Swedman, Andrea Kawabata, and Robert Nelson for their assistance in rearing and collecting the beetles used in this study. We would also like to thank Nicholas Manoukis Grant McQuate, Roxana Myers, and anonymous reviewers for their constructive comments on this manuscript. Funding was provided by the United States Department of Agriculture Agricultural Research Service (USDA-ARS), Hawai'i Department of Agriculture (HDOA) (CBBR&D14), University of Hawai'i College of Tropical Agriculture and Human Resources (UH CTAHR) Project 2040-21000-015-14, and S.B.S. was supported by USDA Farm Bill Project 3.025. NR 16 TC 1 Z9 1 U1 3 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 100 EP 105 DI 10.1093/jee/tov284 PG 6 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800013 PM 26487745 ER PT J AU Yee, WL AF Yee, Wee L. TI Temperature-Mediated Kill and Oviposition of Western Cherry Fruit Fly (Diptera: Tephritidae) in the Presence of Spinosad SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Rhagoletis indifferens; climate; insecticide; activity level; egg laying ID RHAGOLETIS-INDIFFERENS DIPTERA; LONGEVITY; GF-120; FECUNDITY; MORTALITY; SURVIVAL; PROGRAM; BAITS AB Western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), is a quarantine pest of sweet cherry (Prunus avium (L.) L.) that is managed using insecticides, including spinosad, an organic compound that can be applied in low spray volumes. Identifying factors that can increase the efficacy of spinosad can be useful for improving fly control. Here, the major objective was to determine if temperature mediates kill and oviposition of R. indifferens in the presence of low spinosad coverage in the laboratory. Experiments were conducted by placing flies in cages with cherries and with a Petri dish containing 3-12 small spots of dry spinosad at 18.3, 23.9, and 29.4 degrees C. Effects of spinosad rates were also determined. More flies were killed at 23.9 and 29.4 degrees C than at 18.3 degrees C by 1-7 d post exposure. More flies were killed at 29.4 than 23.9 degrees C by 1 d post exposure. However, flies laid more eggs at these temperatures than at 18.3 degrees C. Higher spinosad rates increased kill and decreased oviposition, but even within the highest rate, oviposition was greater at 29.4 than 18.3 degrees C. More flies walked over 5-min observation periods at 29.4 and 23.9 degrees C than 18.3 degrees C, suggesting higher temperatures up to 29.4 degrees C increase kill by increasing fly contact with spinosad as well as increase oviposition rate. Results suggest that spinosad rates in sprays used against R. indifferens should be greater at higher than lower ambient temperatures. C1 [Yee, Wee L.] ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Yee, WL (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov FU United States Department of Agriculture Foreign Agricultural Service FX I thank Janine Jewett, Pete Chapman, Dana Jones, and Nicholas Word (USDA-ARS) for laboratory assistance, and Michael Bush (Washington State University Extension, Yakima, WA) and Juan Roll (Institute de Ecologia, Xalapa, Veracruz, Mexico) for helpful comments on early drafts of the manuscript. I also thank the United States Department of Agriculture Foreign Agricultural Service for funding that partially supported the research. NR 28 TC 0 Z9 0 U1 4 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 132 EP 142 DI 10.1093/jee/tov262 PG 11 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800017 PM 26352751 ER PT J AU ul Haq, I Vreysen, MJB Schutze, M Hendrichs, J Shelly, T AF ul Haq, Ihsan Vreysen, Marc J. B. Schutze, Mark Hendrichs, Jorge Shelly, Todd TI Effects of Methyl Eugenol Feeding on Mating Compatibility of Asian Population of Bactrocera dorsalis (Diptera: Tephritidae) with African Population and with B. carambolae SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bactrocera dorsalis; Bactrocera carambolae; mating compatibility; Bactrocera dorsalis species complex; methyl eugenol ID ORIENTAL FRUIT-FLY; MITOCHONDRIAL-DNA; COMPLEX DIPTERA; WING-SHAPE; SOUTHEAST-ASIA; DACUS-DORSALIS; FLIES DIPTERA; MALES; BEHAVIOR; FEMALES AB Males of some species included in the Bactrocera dorsalis complex are strongly attracted to methyl eugenol (ME) (1,2-dimethoxy-4-(2-propenyl) benzene), a natural compound occurring in a variety of plant species. ME feeding of males of the B. dorsalis complex is known to enhance their mating competitiveness. Within B. dorsalis, recent studies show that Asian and African populations of B. dorsalis are sexually compatible, while populations of B. dorsalis and Bactrocera carambolae are relatively incompatible. The objectives of this study were to examine whether ME feeding by males affects mating compatibility between Asian and African populations of B. dorsalis and ME feeding reduces male mating incompatibility between B. dorsalis (Asian population) and B. carambolae. The data confirmed that Asian and African populations of B. dorsalis are sexually compatible for mating and showed that ME feeding only increased the number of matings. Though ME feeding also increased the number of matings of B. dorsalis (Asian population) and B. carambolae males but the sexual incompatibility between both species was not reduced by treatment with ME. These results conform to the efforts resolving the biological species limits among B. dorsalis complex and have implications for fruit fly control programs in fields and horticultural trade. C1 [ul Haq, Ihsan; Vreysen, Marc J. B.] Insect Pest Control Lab, Joint FAO IAEA Div Nucl Tech Food & Agr, Seibersdorf, Austria. [ul Haq, Ihsan] Natl Agr Res Ctr, Insect Pest Management Program, Pk Rd Islamabad, Islamabad, Pakistan. [Schutze, Mark] Queensland Univ Technol, Sch Earth Environm & Biol Sci, POB 2434, Brisbane, Qld 4001, Australia. [Hendrichs, Jorge] Insect Pest Control Sect, Joint FAO IAEA Div Nucl Tech Food & Agr, Vienna, Austria. [Shelly, Todd] USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. RP ul Haq, I (reprint author), Insect Pest Control Lab, Joint FAO IAEA Div Nucl Tech Food & Agr, Seibersdorf, Austria.; ul Haq, I (reprint author), Natl Agr Res Ctr, Insect Pest Management Program, Pk Rd Islamabad, Islamabad, Pakistan. EM imihsan@yahoo.com; M.Vreysen@iaea.org; m.schutzen@qut.edu.au; j.hendrichs@iaea.org; todd.e.shelly@aphis.usda.gov NR 45 TC 0 Z9 0 U1 4 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 148 EP 153 DI 10.1093/jee/tov274 PG 6 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800019 ER PT J AU Audley, J Mayfield, AE Myers, SW Taylor, A Klingeman, WE AF Audley, J. Mayfield, A. E., III Myers, S. W. Taylor, A. Klingeman, W. E., III TI Phytosanitation Methods Influence Posttreatment Colonization of Juglans nigra Logs by Pityophthorus juglandis (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE walnut twig beetle; forest product; quarantine treatment; thousand cankers disease; bark beetle ID WALNUT TWIG BEETLE; THOUSAND CANKERS DISEASE; GEOSMITHIA-MORBIDA; HEAT-TREATMENT; UNITED-STATES; BLACK-WALNUT AB Several North American walnut species (Juglans spp.) are threatened by thousand cankers disease which is caused by the walnut twig beetle (Pityophthorus juglandis Blackman) and its associated fungal plant pathogen, Geosmithia morbida M. Kolarik, E. Freeland, C. Utley and N. Tisserat sp. nov. Spread of this disease may occur via movement of infested black walnut (Juglans nigra L.) wood. This study evaluated the ability of P. juglandis to colonize J. nigra wood previously treated with various phytosanitation methods. Steam-heated and methyl bromide-fumigated J. nigra logs, as well as kiln-dried natural wane J. nigra lumber (with and without bark) were subsequently exposed to P. juglandis colonization pressure in two exposure scenarios. Following a pheromone-mediated, high-pressure scenario in the canopy of infested trees, beetles readily colonized the bark of steam-heated and methyl bromide-fumigated logs, and were also recovered from kiln-dried lumber on which a thin strip of bark was retained. In the simulated lumberyard exposure experiment, during which samples were exposed to lower P. juglandis populations, beetles were again recovered from bark-on steam-heated logs, but were not recovered from kiln-dried bark-on lumber. These data suggest logs and bark-on lumber treated with phytosanitation methods should not be subsequently exposed to P. juglandis populations. Further beetle exclusion efforts for phytosanitized, bark-on walnut wood products transported out of quarantined areas may be necessary to ensure that these products do not serve as a pathway for the spread of P. juglandis and thousand cankers disease. C1 [Audley, J.; Taylor, A.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, 2506 Jacob Dr, Knoxville, TN 37996 USA. [Mayfield, A. E., III] US Forest Serv, USDA, Southern Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. [Myers, S. W.] USDA APHIS, Ctr Plant Hlth Sci & Technol, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. [Taylor, A.] Univ Tennessee, Dept Plant Sci, 2431 Joe Johnson Dr, Knoxville, TN 37996 USA. RP Audley, J (reprint author), Univ Tennessee, Dept Forestry Wildlife & Fisheries, 2506 Jacob Dr, Knoxville, TN 37996 USA. EM jackson.audley@gmail.com; amayfield02@fs.fed.us; scott.w.myers@aphis.usda.gov; mtaylo29@utk.edu; wklingem@utk.edu FU USDA-Forest Service Forest Health Protection; USDA-Forest Service Southern Research Station; USDA-Animal and Plant Health Inspection Service; University of Tennessee FX We gratefully acknowledge the following individuals and agencies for their assistance on this project: Robert Camp, Katherine McBride (University of Tennessee-Center for Renewable Carbon); Andrew Tait (University of North Carolina Asheville); Paul Merten (United States Department of Agriculture (USDA) Forest Service, Forest Health and Protection); Corinne Fretwell (University of North Carolina at Asheville); Dr. Robert Simpson and Benni Nuchols (University of Tennessee-Holston River Farms Unit); Daren Bailey and the Tennessee Division of Forestry, Knoxville Office; Katherine Nix (University of Tennessee-Entomology and Plant Pathology Department); Wolf Tree Specialists; and several anonymous homeowners in Knox County, TN. This work was funded by USDA-Forest Service Forest Health Protection, USDA-Forest Service Southern Research Station, USDA-Animal and Plant Health Inspection Service, and the University of Tennessee. NR 28 TC 1 Z9 1 U1 3 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 213 EP 221 DI 10.1093/jee/tov252 PG 9 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800029 PM 26318005 ER PT J AU Parys, KA Snodgrass, GL Luttrell, RG Allen, KC Little, NS AF Parys, Katherine A. Snodgrass, Gordon L. Luttrell, Randall G. Allen, K. Clint Little, Nathan S. TI Baseline Susceptibility of Lygus lineolaris (Hemiptera: Miridae) to Novaluron L. SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE tarnished plant bug; novaluron; susceptibility ID TARNISHED PLANT BUG; MISSISSIPPI RIVER DELTA; HETEROPTERA MIRIDAE; HOST PLANTS; INSECTICIDE RESISTANCE; PYRETHROID RESISTANCE; POPULATIONS; BIOASSAY; OVIPOSITION; LOUISIANA AB Tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), populations were collected from field locations in the Mississippi River Delta of Arkansas, Louisiana, and Mississippi. Third-instar F-1 nymphs from each field location, in addition to a laboratory colony, were screened for susceptibility to novaluron. Both a glass vial bioassay and a diet-incorporated bioassay used dose-response regression lines to calculate LC50 and LC90 values for novaluron. Mean LC50s for glass vial bioassays ranged from 44.706 +/- 3.58 to 66.54 +/- 64.19 mu g/vial, while mean LC50s for diet-incorporated bioassays ranged from 12.10 +/- 60.77 to 17.636 +/- 2.42 mu g/200 ml of artificial diet. A comparison of L-C50 values from the same field population screened using both bioassay methods failed to show a relationship. LC50 values from field locations were compared with a historically susceptible population from Crossett, AR. Results indicated that considerable variability in susceptibility to novaluron exists within field populations of tarnished plant bugs across the Delta, including some locations with lower LC50 values than a historically susceptible population. C1 [Parys, Katherine A.; Snodgrass, Gordon L.; Luttrell, Randall G.; Allen, K. Clint; Little, Nathan S.] ARS, USDA, Southern Insect Management Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. RP Parys, KA (reprint author), ARS, USDA, Southern Insect Management Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM katherine.parys@ars.usda.gov; glsnod@gmail.com; randy.luttrell@ars.usda.gov; clint.allen@ars.usda.gov; nathan.little@ars.usda.gov OI Parys, Katherine/0000-0001-7459-3687 FU Adarna Agricultural Solutions Trust [6066-22000-063-07] FX We thank Nakeisha Anderson, Lou Andrews, D'anice Dishmon, Kenya Dixon, Julian Henry, Emily Mosow, Michelle Mullen, Arnett Patterson, Dustin Pickleman, Chad Roberts, and Jana' Slay for their help in both the field and the lab. Special thanks to Deborah Boykin for statistical guidance. Thank you to Maribel Portilla for providing insects from the laboratory colony. Special thanks to Ryan Jackson, Jeff Gore, and Don Cook for their input and feedback. Jesus Esquivel, Maribel Portilla, and three anonymous reviewers provided valuable comments and suggestions to improve the manuscript. This work was partially supported by Adarna Agricultural Solutions (Trust 6066-22000-063-07). Use of commercial and/or trade names does not imply approval or constitute endorsement by the United States Department of Agriculture or the Agricultural Research Service. NR 39 TC 0 Z9 0 U1 2 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 339 EP 344 DI 10.1093/jee/tov318 PG 6 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800045 PM 26546489 ER PT J AU Chen, F Luo, YQ Keena, MA Wu, Y Wu, P Shi, J AF Chen, Fang Luo, Youqing Keena, Melody A. Wu, Ying Wu, Peng Shi, Juan TI DNA Barcoding of Gypsy Moths From China (Lepidoptera: Erebidae) Reveals New Haplotypes and Divergence Patterns Within Gypsy Moth Subspecies SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE gypsy moth; COI; NB system; DNA barcoding; haplotype ID LYMANTRIA-DISPAR; CLADISTIC-ANALYSIS; IDENTIFICATION; POPULATIONS; MARKER AB The gypsy moth from Asia (two subspecies) is considered a greater threat to North America than European gypsy moth, because of a broader host range and females being capable of flight. Variation within and among gypsy moths from China (nine locations), one of the native countries of Asian gypsy moth, were compared using DNA barcode sequences (658 bp of mtDNA cytochrome c oxidase subunit 1 [COI] sequence), together with two restriction site mtDNA markers (NlaIII and BamHI in COI), which is the standard system used to distinguish European gypsy moths from Asian gypsy moths. Relatedness of these populations to gypsy moths from seven other world areas was also examined. The restriction site markers showed that two Chinese populations had both Asian and European haplotypes. DNA barcode sequence divergence between the Asian populations and the European populations was three times greater than the variation within each group. Using Bayesian and parsimonious network analyses, nine previously unknown barcode haplotypes were documented from China and a single haplotype was found to be shared by 55% of the Chinese and some Far Eastern Russian and Japanese individuals. Some gypsy moths from two Chinese populations showed genetic affinity with mtDNA haplotypes from Siberia, Russia, suggesting there could be a cryptic new subspecies in Lymantria dispar (L.) or human-aided movement of moths between these two locations at an earlier point in time. The previously unknown haplotype patterns may complicate efforts to identify Asian gypsy moth introductions and require changes in monitoring and exclusion programs. C1 [Chen, Fang; Luo, Youqing; Wu, Ying; Shi, Juan] Beijing Forestry Univ, Coll Forestry, Beijing Key Lab Forest Pest Control, 35 Qinghua East Rd, Beijing 100083, Peoples R China. [Keena, Melody A.] US Forest Serv, Northern Res Stn, USDA, 51 Mill Pond Rd, Wallingford, CT 06492 USA. [Wu, Peng] Beijing Entry Exit Inspect & Quarantine Bur, 6 Tianshuiyuan St, Beijing 100026, Peoples R China. RP Shi, J (reprint author), Beijing Forestry Univ, Coll Forestry, Beijing Key Lab Forest Pest Control, 35 Qinghua East Rd, Beijing 100083, Peoples R China. EM chen-fang866@163.com; youqingluo@126.com; mkeena@fs.fed.us; 704658127@qq.com; 619049202@qq.com; shi_juan@263.net FU Beijing Higher Education Young Elite Teacher Project [YETP0740]; National Natural Science Foundation of China [31170613]; Fundamental Research for the Central Universities [BLYJ201306]; China Scholarship Council (CSC) FX We are grateful to J.Y. Yu (Guizhou Academy of Forestry), Z.C. Xu (Beijing Forestry University), and M. Takeda (Kobe University) for their kind help in sample collection. We also wish to thank Y. Wu, H.J. Yu, P. Shi, and Y. Li for their participation in rearing moths in the lab and P.F. Li and Havill for their assistance with genetic analysis. This study was funded by Beijing Higher Education Young Elite Teacher Project (YETP0740), the National Natural Science Foundation of China (31170613), the Fundamental Research for the Central Universities (BLYJ201306), and the China Scholarship Council (CSC). NR 37 TC 2 Z9 2 U1 2 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 366 EP 374 DI 10.1093/jee/tov258 PG 9 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800049 PM 26371156 ER PT J AU Shukle, RH Cambron, SE Moniem, HA Schemerhorn, BJ Redding, J Buntin, GD Flanders, KL Reisig, DD Mohammadi, M AF Shukle, Richard H. Cambron, Sue E. Moniem, Hossam Abdel Schemerhorn, Brandon J. Redding, Julie Buntin, G. David Flanders, Kathy L. Reisig, Dominic D. Mohammadi, Mohsen TI Effectiveness of Genes for Hessian Fly (Diptera: Cecidomyiidae) Resistance in the Southeastern United States SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Mayetiola destructor; plant resistance; wheat breeding ID WINTER-WHEAT; CONFERRING RESISTANCE; MAYETIOLA-DESTRUCTOR; VIRULENCE; POPULATIONS; INHERITANCE; GENETICS; MARKERS; IDENTIFICATION; OKLAHOMA AB The Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), is the most important insect pest of wheat (Triticum aestivum L. subsp. aestivum) in the southeastern United States, and the deployment of genetically resistant wheat is the most effective control. However, the use of resistant wheat results in the selection of pest genotypes that can overcome formerly resistant wheat. We have evaluated the effectiveness of 16 resistance genes for protection of wheat from Hessian fly infestation in the southeastern United States. Results documented that while 10 of the genes evaluated could provide protection of wheat, the most highly effective genes were H12, H18, H24, H25, H26, and H33. However, H12 and H18 have been reported to be only partially effective in field evaluations, and H24, H25, and H26 may be associated with undesirable effects on agronomic traits when introgressed into elite wheat lines. Thus, the most promising new gene for Hessian fly resistance appears to be H33. These results indicate that identified highly effective resistance in wheat to the Hessian fly is a limited resource and emphasize the need to identify novel sources of resistance. Also, we recommend that the deployment of resistance in gene pyramids and the development of novel strategies for engineered resistance be considered. C1 [Shukle, Richard H.; Cambron, Sue E.; Schemerhorn, Brandon J.; Redding, Julie] USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Shukle, Richard H.; Cambron, Sue E.; Moniem, Hossam Abdel; Schemerhorn, Brandon J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Moniem, Hossam Abdel] Suez Canal Univ, Fac Sci, Dept Zool, Ismailia 41522, Egypt. [Buntin, G. David] Univ Georgia, Coll Agr Expt Stn, Dept Entomol, Georgia Stn, Griffin, GA 30223 USA. [Flanders, Kathy L.] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Reisig, Dominic D.] N Carolina State Univ, Vernon James Res & Extens Ctr, Dept Entomol, 207 Res Stn Rd, Plymouth, NC 27962 USA. [Mohammadi, Mohsen] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Shukle, RH (reprint author), USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA.; Shukle, RH (reprint author), Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. EM rich.shukle@ars.usda.gov; cambron@purdue.edu; habdelmo@purdue.edu; bschemer@purdue.edu; redding.julie@yahoo.com; gbuntin@uga.edu; flandkl@auburn.edu; dominic_reisig@ncsu.edu; mohamm20@purdue.edu FU USDA-ARS CRIS Project [3602-22000-018-00D] FX This is a joint contribution of the United States Department of Agriculture Agricultural Research Service (USDA-ARS) and Purdue University. This study was supported through USDA-ARS CRIS Project 3602-22000-018-00D. NR 43 TC 0 Z9 0 U1 5 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 399 EP 405 DI 10.1093/jee/tov292 PG 7 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800053 PM 26468515 ER PT J AU Todd, JC Mian, MAR Backus, EA Finer, JJ Redinbaugh, MG AF Todd, Jane C. Mian, M. A. Rouf Backus, Elaine A. Finer, John J. Redinbaugh, Margaret G. TI Feeding Behavior of Soybean Aphid (Hemiptera: Aphididae) Biotype 2 on Resistant and Susceptible Soybean SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Aphis glycines biotype 2; soybean; Rag2 resistance; electrical penetration graph ID SINGLE DOMINANT GENE; SALIVARY SECRETIONS; PENETRATION; PI-243540; STYLETS AB Host plant resistance to the soybean aphid, Aphis glycines Matsumura, is an effective means of controlling populations of this introduced pest species in the United States. Rag (Resistance to Aphis,glycines) genes identified in soybean germplasm have been incorporated into commercial cultivars, but differential responses by soybean aphid biotypes to the Rag genes have made understanding mechanisms underlying resistance associated with Rag genes increasingly important. We compared the behavior of biotype 2 aphids on the resistant soybean line PI243540, which is a source of Rag2, and the susceptible cultivar Wyandot. Scanning electron microscopy revealed that the abaxial surface of leaves from resistant plants had a higher density of both long and glandular trichomes, which might repel aphids, on veins. Time-lapse animation also suggested a repellent effect of resistant plants on aphids. However, electropenatography (EPG) indicated that the time to first probe did not differ between aphids feeding on the resistant and susceptible lines. EPG also indicated that fewer aphids feeding on resistant plants reached the phloem, and the time before reaching the phloem was much longer relative to susceptible soybean. For aphids that reached the phloem, there was no difference in either number of feedings or their duration in phloem. However, aphids feeding on resistant soybean had fewer prolonged phases of active salivation (El) and many more pathway activities and non-probing intervals. Together, the feeding behavior of aphids suggested that Rag2 resistance has strong antixenosis effects, in addition to previously reported antibiosis, and was associated with epidermal and mesophyll tissues. C1 [Todd, Jane C.; Redinbaugh, Margaret G.] ARS Corn, USDA, Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. [Mian, M. A. Rouf; Finer, John J.] Ohio State Univ, Ohio Agr Res & Dev Ctr OSU OARDC, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Backus, Elaine A.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Redinbaugh, Margaret G.] OARDC, OSU, Dept Plant Pathol, Wooster, OH 44691 USA. RP Redinbaugh, MG (reprint author), ARS Corn, USDA, Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA.; Redinbaugh, MG (reprint author), OARDC, OSU, Dept Plant Pathol, Wooster, OH 44691 USA. EM jane.todd@ars.usda.gov; rouf.mian@ars.usda.gov; elaine.backus@ars.usda.gov; finer.1@osu.edu; peg.redinbaugh@ars.usda.gov RI Finer, John/N-4713-2014; Redinbaugh, Margaret/A-3611-2013 OI Finer, John/0000-0001-8004-5468; FU State and Federal funds FX Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or OSU/OARDC. Salaries and research support were provided by State and Federal funds appropriated to The Ohio State University/Ohio Agricultural Research and Development Center. NR 46 TC 1 Z9 1 U1 9 U2 27 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 426 EP 433 DI 10.1093/jee/tov315 PG 8 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800056 PM 26578627 ER PT J AU Armstrong, JS Mornhinweg, DW Payton, ME Puterka, GJ AF Armstrong, J. Scott Mornhinweg, Dolores W. Payton, Mark E. Puterka, Gary J. TI The Discovery of Resistant Sources of Spring Barley, Hordeum vulgare ssp spontaneum, and Unique Greenbug Biotypes SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Hordeum vaigare; Schizaphis graminum,biotype; plant resistance; barley ID DNA SEQUENCE DIVERGENCE; APHIDIDAE BIOTYPES; HOST ASSOCIATIONS; HEMIPTERA; HOMOPTERA; GENES AB The genetic sources for host-plant resistance to the greenbug (Schizaphis graminum Rondani) in barley (Hordeum vulgare ssp. spontaneum) are limited in that only two single dominant genes Rsgl and Rsg2 are available for the complex of greenbug biotypes. We evaluated four new barley lines from the Wild Barley Diversity Collection (WBDC) that previously showed potential for greenbug resistance. Three of those entries, WBDC 53, WBDC 117, WBDC 336, exhibited very dominant sources of resistance to older known biotypes B, C, E, F, H, I, and TX1, which also add to the host -plant differentials used to separate these greenbug biotypes. We also re-evaluated the earlier known set of greenbug biotypes that have been in culture for several years against the known host -plant differentials, and included seven newer greenbug isolates collected from Wyoming to the full complement of small grain differentials. This resulted in the discovery of five new greenbug biotypes, VVY10 MC, VVY81, WY10 B, WY12 MC, and WY86. Wyoming isolates VVY4 A and WY4 B were identical in their phenotypic profile, and should be combined as a single unique greenbug biotype. These barley trials resulted in finding new sources of host -plant resistance, although more research needs to be conducted on what type of resistance was found, and how it can be used. We also document that the Wheatland, Wyoming area serves as a very conducive environment for the development of new greenbug biotypes. C1 [Armstrong, J. Scott; Mornhinweg, Dolores W.; Puterka, Gary J.] ARS, USDA, Wheat Peanut & Other Field Crops Res Unit, 1301 N Western Rd, Stillwater, OK 74075 USA. [Payton, Mark E.] Oklahoma State Univ, Dept Stat, 301B MSCS Bldg, Stillwater, OK 74078 USA. RP Armstrong, JS (reprint author), ARS, USDA, Wheat Peanut & Other Field Crops Res Unit, 1301 N Western Rd, Stillwater, OK 74075 USA. EM scott.armstrong@ars.usda.gov; Do.Mornhinweg@ARS.USDA.GOV; mark.payton@okstate.edu; puterka@ars.usda.gov NR 14 TC 1 Z9 1 U1 2 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 434 EP 438 DI 10.1093/jee/tov320 PG 5 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800057 PM 26527793 ER PT J AU Athanassiou, CG Kavallieratos, NG Campbell, JF AF Athanassiou, Christos G. Kavallieratos, Nickolas G. Campbell, James F. TI Capture of Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) in Floor Traps: The Effect of Previous Captures SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tribolium confusum; Tribolium castaneum; floor trap; monitoring; pheromone ID STORED-PRODUCT INSECTS; FLOUR BEETLES; AGGREGATION PHEROMONE; HERBST; WHEAT; RESPONSES; PEST; FOOD; MILL AB The impact of prior captures on the trapping performance of floor traps was evaluated for the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), and the confused flour beetle, Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae), in laboratory conditions. The effect of trap seeding, adding adults of the same or different species, was evaluated in order to determine possible effects of prior captures in the trap on each species' behavioral responses. The presence of seeded beetles of the same species resulted in an increase in beetle captures for both T. castaneum and T. confusum, but when traps were seeded with the opposite species, there was no increase in beetle captures for either species, and for T. castaneum overall captures in both seeded and unseeded traps was reduced. Overall, T. castaneum tended to have greater captures than T. confusum regardless of the treatment. When the two species were released together, this negated the increased response to seeded traps observed in the single -species treatments. These findings suggest the potential that the presence of beetles in a trap may be influencing the response of beetles in a nearby trap and that T. castaneum and T. confusum when they occur together may influence each other's response to traps. C1 [Athanassiou, Christos G.] Univ Thessaly, Lab Entomol & Agr Zool, Dept Agr Crop Prod & Rural Dev, Phytokou Str, Nea Ionia 38446, Magnissia, Greece. [Athanassiou, Christos G.; Kavallieratos, Nickolas G.; Campbell, James F.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. [Kavallieratos, Nickolas G.] Agr Univ Athens, Lab Agr Zool & Entomol, Dept Crop Sci, 75 Iera Odos Str, GR-11855 Athens, Attica, Greece. [Kavallieratos, Nickolas G.] Benaki Phytopathol Inst, Lab Agr Entomol, Dept Entomol & Agr Zool, 8 Stefanou Delta Str, Kifisia 14561, Attica, Greece. RP Kavallieratos, NG (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA.; Kavallieratos, NG (reprint author), Agr Univ Athens, Lab Agr Zool & Entomol, Dept Crop Sci, 75 Iera Odos Str, GR-11855 Athens, Attica, Greece.; Kavallieratos, NG (reprint author), Benaki Phytopathol Inst, Lab Agr Entomol, Dept Entomol & Agr Zool, 8 Stefanou Delta Str, Kifisia 14561, Attica, Greece. EM athanassiou@agr.uth.gr; nick_kaval@hotmail.com; james.campbell@gars.usda.gov NR 38 TC 0 Z9 0 U1 1 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 461 EP 466 DI 10.1093/jee/tov307 PG 6 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800061 PM 26542957 ER PT J AU Sagel, A Phillips, P Chaudhury, M Skoda, S AF Sagel, Agustin Phillips, Pamela Chaudhury, Muhammad Skoda, Steven TI Managing Ammonia Emissions From Screwworm Larval Rearing Media SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cochliomyia hominivorax; sterile insect technique; insect mass rearing; cellulose fiber; potassium permanganate ID COCHLIOMYIA-HOMINIVORAX; MASS-PRODUCTION; DAIRY MANURE; CALLIPHORIDAE; DIPTERA; DIET AB Mass production, sterilization, and release of screwworms (Cochliomyia hominivorax (Coquerel)) that were competitive in the field significantly contributed to the successful application of the sterile insect technique for eradication of screwworms from continental North America. Metabolic byproducts resulting from protein-rich diets required for larval screwworms lead to ammonia liberation, sometimes at high levels, within the mass rearing facility. Until recently a sodium polyacrylate gel bulking agent was used for the larval media and adsorbed much of the ammonia. A need to replace the gel with an environmentally "friendly" bulking agent, while not increasing ammonia levels in the rearing facility, led to a series of experiments with the objective of developing procedures to reduce ammonia emissions from the larval media bulked with cellulose fiber. Additives of ammonia-converting bacteria, potassium permanganate, and Yucca schidigera Roezl ex Otrgies powder extract, previously reported to reduce ammonia levels in organic environments, were evaluated. Ammonia -converting bacteria did not have a positive effect. Addition of Y. schidigera powder extract (-1% of total volume), potassium permanganate (-250 ppm), and a combination of these two additives (at these same concentrations) kept ammonia at equivalent levels as when larval media was bulked with gel. Potassium permanganate also had sufficient antimicrobial properties that the use of formaldehyde in the diet was not necessary. Further testing is needed, at a mass rearing level, before full implementation into the screwworm eradication program. C1 [Sagel, Agustin] USDA ARS, Screwworm Res Unit, Sterile Screwworm Prod Plant, Pacora, Panama. [Phillips, Pamela; Skoda, Steven] USDA ARS, Screwworm Res Unit, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Chaudhury, Muhammad] USDA ARS, Screwworm Res Unit, UNL EC, 109C Entomol Hall, Lincoln, NE 68583 USA. RP Skoda, S (reprint author), USDA ARS, Screwworm Res Unit, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM agustin.sagel@ars.usda.gov; pamela.phillips@ars.usda.gov; Muhammad.Chaudhury@ars.usda.gov; steve.skoda@ars.usda.gov NR 20 TC 0 Z9 0 U1 2 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2016 VL 109 IS 1 BP 478 EP 483 DI 10.1093/jee/tov291 PG 6 WC Entomology SC Entomology GA DD9VM UT WOS:000370273800064 PM 26468514 ER PT J AU Ayele, GK Gessess, AA Addisie, MB Tilahun, SA Tebebu, TY Tenessa, DB Langendoen, EJ Nicholson, CF Steenhuis, TS AF Ayele, Getaneh K. Gessess, Azalu A. Addisie, Meseret B. Tilahun, Seifu A. Tebebu, Tigist Y. Tenessa, Daregot B. Langendoen, Eddy J. Nicholson, Charles F. Steenhuis, Tammo S. TI A Biophysical and Economic Assessment of a Community-based Rehabilitated Gully in the Ethiopian Highlands SO LAND DEGRADATION & DEVELOPMENT LA English DT Article DE erosion; soil conservation; cost-benefit analysis; sediment; best management practices; Horn of Africa; East Africa ID BLUE NILE BASIN; SOIL-EROSION; NORTHERN ETHIOPIA; WATER; RATES; TECHNOLOGIES; EMPOWERMENT; MANAGEMENT; STABILITY; SEDIMENT AB Gully erosion reduces agricultural productivity by destroying valuable land resources, increases sediment concentrations, reduces water quality, and fills up reservoirs. Gully rehabilitation has proven to be challenging especially in the high-rainfall areas of the Ethiopian Highlands and has therefore had limited success. This paper describes a successful low-cost gully rehabilitation effort with community participation in the Birr watershed in the Blue Nile basin that begun in early 2013. Initially, farmers were reluctant to participate for religious reasons, but with the aid of local priests and respected elders, community discussions, and a visit to a rehabilitated gully, a consensus was reached to rehabilitate a 071-ha upland gully. The rehabilitation measures consisted of regrading the gully head at a 45 degrees slope, constructing low-cost check dams from locally available materials, and planting Pennisetum purpureum grass and Sesbania sesban. At the end of the first post-implementation rainy season, 2,200tons of soil was conserved by the constructed check dams and newly planted vegetation, compared with soil losses of 680 and 560tons in two untreated, nearby gullies. In 2014, an additional 3,100tons of soil was conserved. In 2013, the marginal rate of return (MRR) on the gully rehabilitation investment was 26 based on the value of increased forage production alone. When we include trapped soil nutrient values, the rehabilitation MRR was increased to 10. Although these numbers are impressive, the best proof of the success was that farmers on their own initiative rehabilitated an additional five gullies in 2014. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Ayele, Getaneh K.; Gessess, Azalu A.; Addisie, Meseret B.] Bahir Dar Univ, Fac Civil & Water Resources Engn, PhD Program Integrated Water Management, Bahir Dar, Ethiopia. [Tilahun, Seifu A.; Steenhuis, Tammo S.] Bahir Dar Univ, Sch Civil & Water Resources Engn, Bahir Dar, Ethiopia. [Tebebu, Tigist Y.; Steenhuis, Tammo S.] Cornell Univ, Dept Biol & Environm Engn, 206 Riley Robb Hall, Ithaca, NY 14853 USA. [Tenessa, Daregot B.] Bahir Dar Univ, Dept Econ, Coll Business & Econ, Bahir Dar, Ethiopia. [Langendoen, Eddy J.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS USA. [Nicholson, Charles F.] Penn State Univ, Dept Supply Chain & Informat Syst, University Pk, PA 16802 USA. RP Steenhuis, TS (reprint author), Cornell Univ, Dept Biol & Environm Engn, 206 Riley Robb Hall, Ithaca, NY 14853 USA. EM tss1@cornell.edu OI Langendoen, Eddy/0000-0002-2215-4989 FU USAID [AID-OAA-A-11-00012]; International Foundation for Science (IFS) [W/5383-(9)]; College of Agriculture and Life Sciences of Cornell University; Cornell Institute for Agricultural Development; First Presbyterian Church in Ithaca; Amhara Regional Bureau of Agriculture; Blue Nile Water Institute FX This study was financially supported in part by funds of USAID (AID-OAA-A-11-00012), International Foundation for Science (IFS) (ref. no. W/5383-(9)), an anonymous donor, College of Agriculture and Life Sciences of Cornell University, Cornell Institute for Agricultural Development, the First Presbyterian Church in Ithaca, the Amhara Regional Bureau of Agriculture, and Blue Nile Water Institute. Very special thanks to the people in the Ene-Chilala watershed for their cooperation and hospitality. NR 48 TC 6 Z9 6 U1 4 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1085-3278 EI 1099-145X J9 LAND DEGRAD DEV JI Land Degrad. Dev. PD FEB PY 2016 VL 27 IS 2 BP 270 EP 280 DI 10.1002/ldr.2425 PG 11 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA DD8CJ UT WOS:000370151700017 ER PT J AU Chang, HX Domier, LL Radwan, O Yendrek, CR Hudson, ME Hartman, GL AF Chang, Hao-Xun Domier, Leslie L. Radwan, Osman Yendrek, Craig R. Hudson, Matthew E. Hartman, Glen L. TI Identification of Multiple Phytotoxins Produced by Fusarium virguliforme Including a Phytotoxic Effector (FvNIS1) Associated With Sudden Death Syndrome Foliar Symptoms SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID NECROSIS-INDUCING ACTIVITY; PLATANIN FAMILY PROTEIN; PROGRAMMED CELL-DEATH; STAGONOSPORA-NODORUM; NEP1-LIKE PROTEINS; DISEASE RESISTANCE; COLLETOTRICHUM-ORBICULARE; PHYTOPHTHORA-INFESTANS; FUNCTIONAL-ANALYSIS; MAGNAPORTHE-GRISEA AB Sudden death syndrome (SDS) of soybean is caused by a soil borne pathogen, Fusarium virguliforme. Phytotoxins produced by F. virguliforme are translocated from infected roots to leaves, in which they cause SDS foliar symptoms. In this study, additional putative phytotoxins of F. virguliforme were identified, including three secondary metabolites and 11 effectors. While citrinin, fusaric acid, and radicicol induced foliar chlorosis and wilting, Soybean mosaic virus (SMV)-mediated overexpression of F. virguliforme necrosis-inducing secreted protein 1 (FvNIS1) induced SDS foliar symptoms that mimicked the development of foliar symptoms in the field. The expression level of fvnis1 remained steady over time, although foliar symptoms were delayed compared with the expression levels. SMV::FvNIS1 also displayed genotype specific toxicity to which 75 of 80 soybean cultivars were susceptible. Genome-wide association mapping further identified three single nucleotide polymorphisms at two loci, where three leucine-rich repeat receptor-like protein kinase (LRR-RLK) genes were found. Culture filtrates of fvnis1 knockout mutants displayed a mild reduction in phytotoxicity, indicating that FvNIS1 is one of the phytotoxins responsible for SDS foliar symptoms and may contribute to the quantitative susceptibility of soybean by interacting with the LRR-RLK genes. C1 [Chang, Hao-Xun; Domier, Leslie L.; Radwan, Osman; Yendrek, Craig R.; Hudson, Matthew E.; Hartman, Glen L.] Univ Illinois, Urbana, IL USA. [Domier, Leslie L.; Hartman, Glen L.] ARS, USDA, Urbana, IL USA. [Yendrek, Craig R.] Inst Genom Biol, Urbana, IL USA. RP Hartman, GL (reprint author), Univ Illinois, Urbana, IL USA.; Hartman, GL (reprint author), ARS, USDA, Urbana, IL USA. EM ghartman@illinois.edu RI Hudson, Matthew/A-4438-2008; OI Hudson, Matthew/0000-0002-4737-0936; Chang, Hao-Xun/0000-0002-4667-7741 FU North Central Soybean Research Program; United States Department of Agriculture-Agricultural Research Service FX We thank N. E. McCoppin for development of the SMV expression vector. We would also like to thank C. R. Bowen, J. S. Haudenshield, J.-H. Lee, S.-Y. L. Marzano, and Y. F. Zhao for their feedback on this study and A. E. Lipka for his assistance with the GWAS analysis. The North Central Soybean Research Program and the United States Department of Agriculture-Agricultural Research Service partially funded this project. NR 81 TC 6 Z9 6 U1 8 U2 25 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD FEB PY 2016 VL 29 IS 2 BP 96 EP 108 DI 10.1094/MPMI-09-15-0219-R PG 13 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA DE2OU UT WOS:000370467400002 PM 26646532 ER PT J AU Hao, GX Pitino, M Duan, YP Stover, E AF Hao, Guixia Pitino, Marco Duan, Yongping Stover, Ed TI Reduced Susceptibility to Xanthomonas citri in Transgenic Citrus Expressing the FLS2 Receptor From Nicotiana benthamiana SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID PATTERN-RECOGNITION RECEPTOR; BACTERIAL DISEASE RESISTANCE; RICH REPEAT DOMAIN; FLAGELLIN PERCEPTION; ENHANCES RESISTANCE; CONFERS RESISTANCE; OXIDATIVE BURST; INNATE IMMUNITY; ARABIDOPSIS; KINASE AB Overexpression of plant pattern-recognition receptors by genetic engineering provides a novel approach to enhance plant immunity and broad-spectrum disease resistance. Citrus canker disease associated with Xanthomonas citri is one of the most important diseases damaging citrus production worldwide. In this study, we cloned the FLS2 gene from Nicotiana benthamiana cDNA and inserted it into the binary vector pBinPlus/ARS to transform Hamlin sweet orange and Carrizo citrange. Transgene presence was confirmed by polymerase chain reaction (PCR) and gene expression of NbFLS2 was compared by reverse transcription quantitative PCR. Reactive oxygen species (ROS) production in response to flg22(Xcc), was detected in transgenic Hamlin but not in nontransformed controls. Low or no ROS production was detected from nontransformed Hamlin seedlings challenged with flg22(Xcc). Transgenic plants highly expressing NbFLS2 were selected and were evaluated for resistance to canker incited by X. citri 3213. Our results showed that the integration and expression of the NbFLS2 gene in citrus can increase canker resistance and defense associated gene expression when challenged with X. citri. These results suggest that canker-susceptible Citrus genotypes lack strong basal defense induced by X. citri flagellin and the resistance of these genotypes can be enhanced by transgenic expression of the flagellin receptor from a resistant species. C1 [Hao, Guixia; Pitino, Marco; Duan, Yongping; Stover, Ed] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Hao, GX; Stover, E (reprint author), USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM hao@ars.usda.gov; ed.stover@ars.usda.gov FU Citrus Research and Development Foundation FX We thank E. Cochrane, Y. Avila, and M. Sewell for their excellent technical assistance. Funding was provided by the Citrus Research and Development Foundation. We also thank G. Martin from Cornell University for N. benthamiana FLS2 information. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 53 TC 5 Z9 5 U1 2 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD FEB PY 2016 VL 29 IS 2 BP 132 EP 142 DI 10.1094/MPMI-09-15-0211-R PG 11 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA DE2OU UT WOS:000370467400005 PM 26554734 ER PT J AU Burri, BJ La Frano, MR Zhu, CH AF Burri, Betty J. La Frano, Michael R. Zhu, Chenghao TI Absorption, metabolism, and functions of beta-cryptoxanthin SO NUTRITION REVIEWS LA English DT Review DE antioxidant; beta-cryptoxanthin; bone; cancer; vitamin A ID SERUM CAROTENOID CONCENTRATIONS; SINGLE NUCLEOTIDE POLYMORPHISMS; RATS IN-VIVO; GERBILS MERIONES-UNGUICULATUS; APOPTOTIC CELL-DEATH; FLESHED SWEET-POTATO; BONE-MINERAL DENSITY; A POOL SIZE; VITAMIN-A; LUNG-CANCER AB beta-carotene-rich-Cryptoxanthin, a carotenoid found in fruits and vegetables such as tangerines, red peppers, and pumpkin, has several functions important for human health. Most evidence from observational, in vitro, animal model, and human studies suggests that beta-cryptoxanthin has relatively high bioavailability from its common food sources, to the extent that some beta-cryptoxanthin-rich foods might be equivalent to beta-carotene-rich foods as sources of retinol. beta-Cryptoxanthin is an antioxidant in vitro and appears to be associated with decreased risk of some cancers and degenerative diseases. In addition, many in vitro, animal model, and human studies suggest that beta-cryptoxanthin-rich foods may have an anabolic effect on bone and, thus, may help delay osteoporosis. C1 [Burri, Betty J.; La Frano, Michael R.; Zhu, Chenghao] Univ Calif Davis, Western Human Nutr Res Ctr, USDA ARS, Dept Nutr, Davis, CA 95616 USA. RP Burri, BJ (reprint author), USDA ARS, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM bburri@pacbell.net OI La Frano, Michael/0000-0002-2269-1688 NR 162 TC 3 Z9 3 U1 11 U2 28 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD FEB PY 2016 VL 74 IS 2 BP 69 EP 82 DI 10.1093/nutrit/nuv064 PG 14 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DE0FW UT WOS:000370301300001 PM 26747887 ER PT J AU Vaughn, AE Ward, DS Fisher, JO Faith, MS Hughes, SO Kremers, SPJ Musher-Eizenman, DR O'Connor, TM Patrick, H Power, TG AF Vaughn, Amber E. Ward, Dianne S. Fisher, Jennifer O. Faith, Myles S. Hughes, Sheryl O. Kremers, Stef P. J. Musher-Eizenman, Dara R. O'Connor, Teresia M. Patrick, Heather Power, Thomas G. TI Fundamental constructs in food parenting practices: a content map to guide future research SO NUTRITION REVIEWS LA English DT Review DE children; eating behaviors; feeding practices; obesity ID CHILD-FEEDING QUESTIONNAIRE; CONFIRMATORY FACTOR-ANALYSIS; FAMILY ROUTINES INVENTORY; SOFT DRINK CONSUMPTION; NON-CORE FOOD; EATING BEHAVIORS; VEGETABLE CONSUMPTION; PRESCHOOL-CHILDREN; DIETARY-INTAKE; SNACKING BEHAVIOR AB Although research shows that "food parenting practices" can impact children's diet and eating habits, current understanding of the impact of specific practices has been limited by inconsistencies in terminology and definitions. This article represents a critical appraisal of food parenting practices, including clear terminology and definitions, by a working group of content experts. The result of this effort was the development of a content map for future research that presents 3 overarching, higher-order food parenting constructs - coercive control, structure, and autonomy support - as well as specific practice subconstructs. Coercive control includes restriction, pressure to eat, threats and bribes, and using food to control negative emotions. Structure includes rules and limits, limited/guided choices, monitoring, meal-and snacktime routines, modeling, food availability and accessibility, food preparation, and unstructured practices. Autonomy support includes nutrition education, child involvement, encouragement, praise, reasoning, and negotiation. Literature on each construct is reviewed, and directions for future research are offered. Clear terminology and definitions should facilitate cross-study comparisons and minimize conflicting findings resulting from previous discrepancies in construct operationalization. C1 [Vaughn, Amber E.; Ward, Dianne S.] Univ N Carolina, Ctr Hlth Promot & Dis Prevent, Chapel Hill, NC USA. [Ward, Dianne S.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Nutr, Chapel Hill, NC USA. [Fisher, Jennifer O.] Temple Univ, Ctr Obes Res & Educ, Philadelphia, PA 19122 USA. [Faith, Myles S.] SUNY Buffalo, Grad Sch Educ, Dept Counseling Sch & Educ Psychol, Buffalo, NY 14260 USA. [Hughes, Sheryl O.; O'Connor, Teresia M.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Kremers, Stef P. J.] Maastricht Univ, NUTRIM Sch Nutr & Translat Res Metab, Dept Hlth Promot, NL-6200 MD Maastricht, Netherlands. [Musher-Eizenman, Dara R.] Bowling Green State Univ, Dept Psychol, Bowling Green, OH 43403 USA. [Patrick, Heather] LiveHealthier Inc, Bethesda, MD USA. [Power, Thomas G.] Washington State Univ, Dept Human Dev, Pullman, WA 99164 USA. RP Vaughn, AE (reprint author), 1700 Martin L King Jr Blvd,CB 7426, Chapel Hill, NC 27599 USA. EM avaughn@email.unc.edu FU Center for Health Promotion and Disease Prevention at the University of North Carolina at Chapel Hill, a Prevention Research Center through Centers for Disease Control and Prevention [U48-DP001944] FX This project was supported by the Center for Health Promotion and Disease Prevention at the University of North Carolina at Chapel Hill, a Prevention Research Center funded through a cooperative agreement with the Centers for Disease Control and Prevention (U48-DP001944). The findings and conclusions in this journal article are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention. NR 176 TC 11 Z9 11 U1 8 U2 27 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD FEB PY 2016 VL 74 IS 2 BP 98 EP 117 DI 10.1093/nutrit/nuv061 PG 20 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DE0FW UT WOS:000370301300003 PM 26724487 ER PT J AU Lu, SW Edwards, MC AF Lu, Shunwen Edwards, Michael C. TI Genome-Wide Analysis of Small Secreted Cysteine-Rich Proteins Identifies Candidate Effector Proteins Potentially Involved in Fusarium graminearum-Wheat Interactions SO PHYTOPATHOLOGY LA English DT Article DE fungal pathogenesis; gene-for-gene interactions; host resistance; virulence factors; wheat scab disease ID HOST-SELECTIVE TOXINS; COCHLIOBOLUS-HETEROSTROPHUS; STAGONOSPORA-NODORUM; VIRULENCE FACTOR; USTILAGO-MAYDIS; HEAD BLIGHT; GENE; INFECTION; PATHOGENICITY; RESISTANCE AB Pathogen-derived, small secreted cysteine-rich proteins (SSCPs) are known to be a common source of fungal effectors that trigger resistance or susceptibility in specific host plants. This group of proteins has not been well studied in Fusarium graminearum, the primary cause of Fusarium head blight (FHB), a devastating disease of wheat. We report here a comprehensive analysis of SSCPs encoded in the genome of this fungus and selection of candidate effector proteins through proteomics and sequence/transcriptional analyses. A total of 190 SSCPs were identified in the genome of F. graminearum (isolate PH-1) based on the presence of N-terminal signal peptide sequences, size (<= 200 amino acids), and cysteine content (>= 2%) of the mature proteins. Twenty-five (approximately 13%) SSCPs were confirmed to be true extracellular proteins by nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) analysis of a minimal medium-based in vitro secretome. Sequence analysis suggested that 17 SSCPs harbor conserved functional domains, including two homologous to Ecp2, a known effector produced by the tomato pathogen Cladosporium fulvum. Transcriptional analysis revealed that at least 34 SSCPs (including 23 detected in the in vitro secretome) are expressed in infected wheat heads; about half are up-regulated with expression patterns correlating with the development of FHB. This work provides a solid candidate list for SSCP-derived effectors that may play roles in mediating F. graminearum-wheat interactions. The in vitro secretome-based method presented here also may be applicable for identifying candidate effectors in other ascomycete pathogens of crop plants. C1 [Lu, Shunwen; Edwards, Michael C.] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Lu, SW (reprint author), USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. EM Shunwen.Lu@ars.usda.gov FU USDA-ARS CRIS [5442-21000-037-00D] FX We thank C. Steen and M. Miller for technical assistance. This study was supported by USDA-ARS CRIS project 5442-21000-037-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 2 Z9 3 U1 6 U2 24 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2016 VL 106 IS 2 BP 166 EP 176 DI 10.1094/PHYTO-09-15-0215-R PG 11 WC Plant Sciences SC Plant Sciences GA DD8YV UT WOS:000370213700007 PM 26524547 ER PT J AU Tian, Y Zhan, GM Chen, XM Tungruentragoon, A Lu, X Zhao, J Huang, LL Kang, ZS AF Tian, Yuan Zhan, Gangming Chen, Xianming Tungruentragoon, Angkana Lu, Xia Zhao, Jie Huang, Lili Kang, Zhensheng TI Virulence and Simple Sequence Repeat Marker Segregation in a Puccinia striiformis f. sp tritici Population Produced by Selfing a Chinese Isolate on Berberis shensiana SO PHYTOPATHOLOGY LA English DT Article ID WHEAT STRIPE RUST; US PACIFIC-NORTHWEST; ALTERNATE HOST; MICROSATELLITE LOCI; UNITED-STATES; F.SP TRITICI; INHERITANCE; PATHOGEN; FUNGUS; RECOMBINATION AB Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust, frequently produces new races overcoming resistance in wheat cultivars. A recently identified race, V26 with virulence to Yr26 and many other stripe rust resistance genes, has a high potential to cause epidemics in China. In this study, teliospores from a single-urediniospore isolate of V26 (Pinglan 17-7) produced on the wheat line 92R137 (Yr26) were used to produce a sexual population through selling by infecting Berberis shensiana plants under controlled conditions. One hundred and eighteen progeny isolates and the parental isolate were phenotyped for virulence/avirulence on 24 Yr gene lines of wheat. These progeny isolates were all avirulent to Yr5, Yr8, Yr15, and YrTr1 and virulent to Yr1, Yr2, Yr7, Yr9, Yr10, Yr17, Yr24, Yr25, Yr26, YrA, YrExp2, and YrV23, indicating that the parental isolate is homozygous avirulent or homozygous virulent at these loci. The progeny population segregated for avirulence to Yr6, Yr43, and YrSP at one locus (3 avirulent:1 virulent ratio); for virulence to Yr27 and Yr28 at one locus (3 virulent:1 avirulent); and for Yr4, Yr32, and Yr44 at two loci (15 virulent:1 avirulent). Among the eight segregating avirulence/virulence loci, association was found between virulence to Yr4 and Yr32, as well as between virulence to Yr6 and Yr43 based on chi(2) tests. From 82 genotypically different progeny isolates, 24 pathotypes and 82 multi locus genotypes were identified. The results show that a highly diverse population can be produced from a single isolate by selfing on a barberry plant and sexually produced population can be used to genetically characterize virulence of the stripe rust pathogen. C1 [Tian, Yuan; Zhan, Gangming; Tungruentragoon, Angkana; Lu, Xia; Zhao, Jie; Huang, Lili; Kang, Zhensheng] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Tian, Yuan; Zhan, Gangming; Tungruentragoon, Angkana; Lu, Xia; Zhao, Jie; Huang, Lili; Kang, Zhensheng] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China. [Chen, Xianming] Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Huang, LL; Kang, ZS (reprint author), Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China.; Huang, LL; Kang, ZS (reprint author), Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China. EM huanglili@nwsuaf.edu.cn; kangzs@nwsuaf.edu.cn FU National Key Basic Research Program of China [2013CB127700]; International Science & Technology Cooperation Program of China [2011DFG32990]; Nature Science Foundation of China [31371882, 31271986]; Modern Agro-industry Technology Research System in China [CARS-3-1-11]; Ministry of Education of China [B07049] FX This work was supported by the National Key Basic Research Program of China (2013CB127700), International Science & Technology Cooperation Program of China (2011DFG32990), the Nature Science Foundation of China (31371882, 31271986), Modern Agro-industry Technology Research System in China (CARS-3-1-11), and the 111 Project from the Ministry of Education of China (B07049). NR 55 TC 3 Z9 4 U1 4 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2016 VL 106 IS 2 BP 185 EP 191 DI 10.1094/PHYTO-07-15-0162-R PG 7 WC Plant Sciences SC Plant Sciences GA DD8YV UT WOS:000370213700009 PM 26551448 ER PT J AU Crespo, M Cazorla, FM de Vicente, A Arrebola, E Tores, JA Maymon, M Freeman, S Aoki, T O'Donnell, K AF Crespo, M. Cazorla, F. M. de Vicente, A. Arrebola, E. Tores, J. A. Maymon, M. Freeman, S. Aoki, T. O'Donnell, K. TI Analysis of Genetic Diversity of Fusarium tupiense, the Main Causal Agent of Mango Malformation Disease in Southern Spain SO PLANT DISEASE LA English DT Article ID FUJIKUROI SPECIES COMPLEX; GIBBERELLA-FUJIKUROI; 1ST REPORT; MANGIFERAE; OXYSPORUM; MEXICO; BRAZIL; PSEUDOCIRCINATUM; IDENTIFICATION; SUBGLUTINANS AB Mango malformation disease (MMD) has become an important global disease affecting this crop. The aim of this study was to identify the main causal agents of MMD in the Axarquia region of southern Spain and determine their genetic diversity. Fusarium nzangiferae was previously described in the Axarquia region but it represented only one-third of the fusaria recovered from malformed trees. In the present work, fusaria associated with MMD were analyzed by arbitrary primed polymerase chain reaction (ap-PCR), random amplified polymorphic DNA (RAPD), vegetative compatibility grouping (VCG), a PCR screen for mating type idiomorph, and phylogenetic analyses of multilocus DNA sequence data to identify and characterize the genetic diversity of the MMD pathogens. These analyses confirmed that 92 of the isolates were F. tupiense, which was previously only known from Brazil and Senegal. In addition, two isolates of a putatively novel MMD pathogen were discovered, nested within the African Glade of the Fusarium fujikuroi species complex. The F. tupiense isolates all belonged to VCG I, which was first described in Brazil, and the 11 isolates tested showed pathogenicity on mango seedlings. Including the prior discovery of F. mangiferae, three exotic MMD pathogenic species have been found in southern Spain, which suggests multiple independent introductions of MAID pathogens in the Axarquia region. C1 [Crespo, M.; Cazorla, F. M.; de Vicente, A.] Univ Malaga, Fac Ciencias, Inst Hortofruticultura Subtrop & Mediterranea La, Dept Microbiol,CSIC, Campus Teatinos S-N, E-29071 Malaga, Spain. [Arrebola, E.; Tores, J. A.] Univ Malaga, Inst Hortofruticultura Subtrop & Mediterranea La, Estn Expt La Mayora, CSIC, Malaga 29750, Spain. [Maymon, M.] Agr Res Org, Dept Plant Pathol & Weed Res, Volcani Ctr, IL-50250 Bet Dagan, Israel. [Aoki, T.] Natl Inst Agrobiol Sci, Genet Resources Ctr MAFF, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan. [O'Donnell, K.] USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, NCAUR, Peoria, IL 61604 USA. RP de Vicente, A (reprint author), Univ Malaga, Fac Ciencias, Inst Hortofruticultura Subtrop & Mediterranea La, Dept Microbiol,CSIC, Campus Teatinos S-N, E-29071 Malaga, Spain. EM adevicente@uma.es RI Cazorla, Francisco/G-3907-2011; de Vicente, Antonio/G-4405-2011 OI Cazorla, Francisco/0000-0003-0798-0964; de Vicente, Antonio/0000-0003-2716-9861 FU CICE-Junta de Andalucia, Proyecto de Excelencia - FEDER funds (EU) [P07-AGR-02471, P12-AGR-1473]; University of Malaga; [SAT-2803 TROPS] FX This research was supported by grants from CICE-Junta de Andalucia, Proyecto de Excelencia P07-AGR-02471 and P12-AGR-1473, co-funded by FEDER funds (EU). This work was also financed by a grant from SAT-2803 TROPS, Reyes Gutierrez S. L. and Viveros Brokaw S. L. We thank J. M. Hermoso, E. Guirado, J. Gonzalez (IHSM La Mayora-UMA-CSIC), and D. Sarmiento, (SAT-2803 TROPS) for their contributions to the pathogen survey. M. Crespo was also supported by a grant from the University of Malaga for a short stay in the Volcani Center, Bet Dagan, Israel. NR 45 TC 2 Z9 2 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2016 VL 100 IS 2 BP 276 EP 286 DI 10.1094/PDIS-02-15-0153-RE PG 11 WC Plant Sciences SC Plant Sciences GA DE0HI UT WOS:000370305300005 ER PT J AU Li, JB Lu, L Jia, YL Wang, Q Fukuta, Y Li, CY AF Li, Jinbin Lu, Lin Jia, Yulin Wang, Qun Fukuta, Yoshimichi Li, Chengyun TI Characterization of Field Isolates of Magnaporthe oryzae with Mating Type, DNA Fingerprinting, and Pathogenicity Assays SO PLANT DISEASE LA English DT Article ID RICE BLAST FUNGUS; SEQUENCE REPEAT MARKERS; MYCOSPHAERELLA-GRAMINICOLA; GRISEA POPULATIONS; FERTILITY STATUS; FINGER MILLET; MICROSATELLITE MARKERS; PYRICULARIA-GRISEA; GENETIC-ANALYSIS; DIVERSITY AB Due to the harmful nature of the rice blast fungus, Magnaporthe oryzae, it is beneficial to characterize field isolates to help aid in the deployment of resistance (R) genes in rice. In the present study, 252 field isolates of M. oryzae, collected from rice fields of Yunnan Province in China, were assessed for mating type, DNA fingerprinting, and disease reactions to differential rice lines. In total, 94 isolates (37.3%) were MAT1-1 and 158 (62.7%) were MAT1-2 based on polymerase chain reaction assays, and some of them were verified with the tester isolates. All MAT1-1 and MAT1-2 isolates were virulent to some of the International Rice Research Institute Japan International Research Center for Agricultural Sciences monogenic lines harboring 22 major resistance genes as differential varieties. Three simple-sequence repeat markers were used to examine genetic diversity in all isolates. The existence of regional patterns of genetic diversity, sexual reproduction potential, and pathogenicity suggests that M. oryzae populations have been independently asexually adapted in rice fields during crop cultivation. C1 [Li, Jinbin] Yunnan Acad Agr Sci, Agr Environm & Resources Res Inst, Kunming 650205, Yunnan Province, Peoples R China. [Lu, Lin] YAAS, Flower Res Inst, Kunming, Peoples R China. [Jia, Yulin] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Wang, Qun] YAAS, Agr Environ & Resources Res Inst, Kunming, Peoples R China. [Fukuta, Yoshimichi] Japan Int Res Ctr Agr Sci, Trop Agr Res Front, Ishigaki, Okinawa 9070002, Japan. [Li, Chengyun] Yunnan Agr Univ, Minist Educ, Key Lab Agr Biodivers & Pest Management, Kunming 650201, Yunnan Province, Peoples R China. RP Jia, YL (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA.; Li, CY (reprint author), Yunnan Agr Univ, Minist Educ, Key Lab Agr Biodivers & Pest Management, Kunming 650201, Yunnan Province, Peoples R China. EM Yulin.Jia@ars.usda.gov; licheng_yun@163.com FU National Natural Science Foundation of China [31460454, 31160355]; National Basic Research Program [2012CB722901]; Department of Sciences and Technology of Yunnan Province [2015HB076] FX We thank Y. Zhu and Y. Guo of Yunnan Agricultural University for useful discussions and T. Bianco of the United States Department of Agriculture-Agricultural Research Service Dale Bumpers National Rice Research Center for proofreading and useful discussion. Financial support was provided by the National Natural Science Foundation of China (31460454 and 31160355), National Basic Research Program 973 (2012CB722901), and the Department of Sciences and Technology of Yunnan Province (2015HB076). NR 60 TC 0 Z9 0 U1 4 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2016 VL 100 IS 2 BP 298 EP 303 DI 10.1094/PDIS-06-15-0660-RE PG 6 WC Plant Sciences SC Plant Sciences GA DE0HI UT WOS:000370305300008 ER PT J AU Byamukama, E Tatineni, S Hein, G McMechan, J Wegulo, SN AF Byamukama, E. Tatineni, S. Hein, G. McMechan, J. Wegulo, S. N. TI Incidence of Wheat streak mosaic virus, Triticum mosaic virus, and Wheat mosaic virus in Wheat Curl Mites Recovered from Maturing Winter Wheat Spikes SO PLANT DISEASE LA English DT Article ID HIGH-PLAINS VIRUS; DIFFERENTIAL TRANSMISSION; GREAT-PLAINS; IDENTIFICATION; POPULATIONS; VECTOR; YIELD; FIELD AB Wheat curl mites (WCM; Aceria tosichella) transmit Wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), and Wheat mosaic virus (WMoV) to wheat (Triticum aestivum L.) in the Great Plains region of the United States. These viruses can be detected in single, double, or triple combinations in leaf samples. Information on incidence of viruses in WCM at the end of the growing season is scant. The availability of this information can enhance our knowledge of the epidemiology of WCM-transmitted viruses. This research was conducted to determine the frequency of occurrence of WSMV, TriMV, and WMoV in WCM populations on field collected maturing wheat spikes and to determine differences in WCM densities in three geographical regions (southeast, west-central, and panhandle) in Nebraska. Maturing wheat spikes were collected from 83 fields across Nebraska in 2011 and 2012. The spikes were placed in proximity to wheat seedlings (three-to four-leaf stage) in WCM-proof cages in a growth chamber and on sticky tape. WCM that moved off the drying wheat spikes in cages infested the wheat seedlings. WCM that moved off wheat spikes placed on sticky tape were trapped on the tape and were counted under a dissecting microscope. At 28 days after infestation, the wheat plants were tested for the presence of WSMV, TriMV, or WMoV using enzyme-linked immunosorbent assay and multiplex polymerase chain reaction. WSMV was the most predominant virus detected in wheat seedlings infested with WCM from field-collected spikes. Double (TriMV+WSMV or WMoV+WSMV) or triple (TriMV+WMoV+WSMV) virus detections were more frequent (47%) than single detections (5%) of TriMV or WSMV. Overall, 81% of the wheat seedlings infested with WCM tested positive for at least one virus. No significant association (P > 0.05) was found between regions for WCM trapped on tape. These results suggest that WCM present on mature wheat spikes harbor multiple wheat viruses and may explain high virus incidence when direct movement of WCM into emerging winter wheat occurs in the fall. C1 [Byamukama, E.; Tatineni, S.; Wegulo, S. N.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Tatineni, S.] USDA ARS, Washington, DC 20250 USA. [Hein, G.; McMechan, J.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Byamukama, E.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. RP Wegulo, SN (reprint author), Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. EM swegulo2@unl.edu FU Agriculture and Food Research Initiative Competitive Grants Program Grant of the National Institute of Food and Agriculture [2010-85605-20546] FX We thank R. Havercamp, J. Horell, J. Stevens, and J. Milhouse for their assistance in sample collection and virus testing. Funding for this work was provided by the Agriculture and Food Research Initiative Competitive Grants Program Grant Number 2010-85605-20546 of the National Institute of Food and Agriculture. NR 28 TC 0 Z9 0 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2016 VL 100 IS 2 BP 318 EP 323 DI 10.1094/PDIS-06-15-0692-RE PG 6 WC Plant Sciences SC Plant Sciences GA DE0HI UT WOS:000370305300011 ER PT J AU Babiker, EM Gordon, TC Bonman, JM Chao, S Rouse, MN Brown-Guedira, G Williamson, S Pretorius, ZA AF Babiker, E. M. Gordon, T. C. Bonman, J. M. Chao, S. Rouse, M. N. Brown-Guedira, G. Williamson, S. Pretorius, Z. A. TI Rapid Identification of Resistance Loci Effective Against Puccinia graminis f. sp tritici Race TTKSK in 33 Spring Wheat Landraces SO PLANT DISEASE LA English DT Article ID STEM RUST RESISTANCE; BULKED SEGREGANT ANALYSIS; TETRAPLOID WHEAT; UNITED-STATES; PCR MARKERS; GENES SR24; US WHEAT; UG99; GERMPLASM; VIRULENCE AB Wheat breeders worldwide are seeking new sources of resistance to Puccinia graminis f. sp. tritici race TTKSK. To prioritize field-resistant landraces for follow-up genetic studies to test for the presence of new resistance genes, seedling response to P. graminis f. sp. tritici race TTKSK, molecular markers linked to specific Sr genes, segregation ratios among progeny from crosses, and bulked segregant analyses (BSA) were used. In total, 33 spring wheat landraces with seedling resistance to P. graminis f. sp. tritici race TTKSK were crossed to a susceptible genotype, LMPG-6. The segregation ratios of stem rust reactions in F-2 seedlings fit a single dominant gene model in 31 populations and progeny from two crosses gave ambiguous results. Using the 90K wheat single-nucleotide polymorphism genotyping platform, BSA showed that the seedling resistance in 29 accessions is probably controlled by loci on chromosome 2BL. For the three remaining accessions, BSA revealed that the seedling resistance is most likely controlled by previously unreported genes. For confirmation, two populations were advanced to the F-2:3 and screened against P. graminis f. sp. tritici race TTKSK. Segregation of the F-2:3 families fit a 1:2:1 ratio for a single dominant gene. Using the F-2:3 families, BSA located the TTKSK locus on chromosome 6DS to the same location as Sr42. C1 [Babiker, E. M.; Gordon, T. C.; Bonman, J. M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Chao, S.] USDA ARS, Cereal Crops Res, Fargo, ND 58102 USA. [Rouse, M. N.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Brown-Guedira, G.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27606 USA. [Williamson, S.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Pretorius, Z. A.] Univ Orange Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. RP Bonman, JM (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. EM Mike.Bonman@ars.usda.gov RI Rouse, Matthew/G-5474-2011 FU USDA-ARS National Plant Disease Recovery System; USDA-ARS CRIS project [2050-21000-029-00D]; Durable Rust Resistance in Wheat (DRRW) project; Bill and Melinda Gates Foundation; United Kingdom Department for International development; National Research Initiative from USDA National Institute of Food and Agriculture [2011-68002-30029] FX We thank S. Stoxen and S. Gale for their technical assistance. This research was supported by the USDA-ARS National Plant Disease Recovery System, the USDA-ARS CRIS project 2050-21000-029-00D, and the Durable Rust Resistance in Wheat (DRRW) project managed by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International development. This work was supported, in part, by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. NR 42 TC 2 Z9 2 U1 3 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2016 VL 100 IS 2 BP 331 EP 336 DI 10.1094/PDIS-04-15-0466-RE PG 6 WC Plant Sciences SC Plant Sciences GA DE0HI UT WOS:000370305300013 ER PT J AU Patpour, M Hovmoller, MS Justesen, AF Newcomb, M Olivera, P Jin, Y Szabo, LJ Hodson, D Shahin, AA Wanyera, R Habarurema, I Wobibi, S AF Patpour, M. Hovmoller, M. S. Justesen, A. F. Newcomb, M. Olivera, P. Jin, Y. Szabo, L. J. Hodson, D. Shahin, A. A. Wanyera, R. Habarurema, I. Wobibi, S. TI Emergence of Virulence to SrTmp in the Ug99 Race Group of Wheat Stem Rust, Puccinia graminis f. sp tritici, in Africa. SO PLANT DISEASE LA English DT News Item C1 [Patpour, M.; Hovmoller, M. S.; Justesen, A. F.] Aarhus Univ, DK-4200 Slagelse, Denmark. [Newcomb, M.; Olivera, P.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Jin, Y.; Szabo, L. J.] USDA ARS, St Paul, MN USA. [Hodson, D.] CIMMYT, Addis Ababa, Ethiopia. [Shahin, A. A.] Plant Pathol Res Inst, Sakha 33717, Kafrelsheikh, Egypt. [Wanyera, R.] KALRO Plant Breeding Res Ctr, Njoro, Kenya. [Habarurema, I.] Rwanda Agr Board, POB 5016, Kigali, Rwanda. [Wobibi, S.] BZARDI, POB 1356, Mbale, Uganda. RP Patpour, M (reprint author), Aarhus Univ, DK-4200 Slagelse, Denmark. RI Hovmoller, Mogens/E-8374-2016; Justesen, Annemarie/E-9853-2016 NR 3 TC 8 Z9 8 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2016 VL 100 IS 2 BP 522 EP 522 DI 10.1094/PDIS-06-15-0668-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DE0HI UT WOS:000370305300054 ER PT J AU Butler, S Young-Kelly, H Raper, T Cochran, A Jordan, J Shrestha, S Lamour, K Mengistu, A Rocha, AC Shelby, P AF Butler, S. Young-Kelly, H. Raper, T. Cochran, A. Jordan, J. Shrestha, S. Lamour, K. Mengistu, A. Rocha, A. C. Shelby, P. TI First Report of Target Spot Caused by Corynespora cassiicola on Cotton in Tennessee. SO PLANT DISEASE LA English DT News Item C1 [Butler, S.; Young-Kelly, H.; Raper, T.; Cochran, A.; Jordan, J.] Univ Tennessee, Jackson, TN USA. [Shrestha, S.; Lamour, K.] Univ Tennessee, Knoxville, TN USA. [Mengistu, A.] USDA ARS, Crop Genet Res Unit, Jackson, TN USA. [Rocha, A. C.] Univ Autonoma Ciudad, Juarez, Mexico. [Shelby, P.] Univ Tennessee, Trenton, NJ USA. RP Butler, S (reprint author), Univ Tennessee, Jackson, TN USA. NR 5 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2016 VL 100 IS 2 BP 535 EP 535 DI 10.1094/PDIS-07-15-0785-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DE0HI UT WOS:000370305300087 ER PT J AU Fickas, KC Cohen, WB Yang, ZQ AF Fickas, Kate C. Cohen, Warren B. Yang, Zhiqiang TI Landsat-based monitoring of annual wetland change in the Willamette Valley of Oregon, USA from 1972 to 2012 SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Remote sensing; Wetlands; MSS; Tasseled Cap; No-net-loss; Change detection ID TIME-SERIES DATA; FOREST DISTURBANCE; PACIFIC-NORTHWEST; DETECTING TRENDS; INVENTORY MAPS; TM IMAGERY; CLASSIFICATION; VEGETATION; ECOSYSTEMS; IDENTIFICATION AB In Oregon's Willamette Valley, remaining wetlands are at high risk to loss and degradation from agricultural activity and urbanization. With an increased need for fine temporal-scale monitoring of sensitive wetlands, we used annual Landsat MSS and TM/ETM+ images from 1972 to 2012 to manually interpret loss, gain, and type conversion of wetland area in the floodplain of the Willamette River. By creating Tasseled Cap Brightness, Greenness, and Wetness indices for MSS data that visually match TM/ETM+ Tasseled Cap images, we were able to construct a complete and consistent, annual time series and utilize the entire Landsat archive. With an extended time series we were also able to compare annual trends of net change in wetland area before and after the no-net-loss policy established under Section 404 of the Clean Water Act in 1990 using a Theil-Sen Slope estimate analysis. Vegetated wetlands experienced a 314 ha net loss of wetland area and non-vegetated wetlands experienced a 393 ha net gain, indicating higher functioning wetlands were replaced in area by non-vegetated wetland habitats such as agricultural and quarry ponds. The majority of both gain and loss in the study area was attributed to gains and losses of agricultural land. After 1990 policy implementations, the rate of wetland area lost slowed for some wetland categories and reversed into trends of gain in wetland area for others, perhaps representative of the success of increased regulations. Overall accuracy of land use classification through manual interpretation was at 80 %. This accuracy increased to 91.1 % when land use classes were aggregated to either wetland or upland categories, indicating that our methodology was more accurate at distinguishing between general upland and wetland than finer categorical classes. C1 [Fickas, Kate C.; Yang, Zhiqiang] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Cohen, Warren B.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Fickas, KC (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. EM kate.fickas@oregonstate.edu FU USDA Forest Service Pacific Northwest Research Station FX Funding for this research was provided by the USDA Forest Service Pacific Northwest Research Station. We would like to thank River Design Group, Inc. for the use of their floodplain inundation map in our study. The authors also acknowledge, with gratitude, the detailed and valuable comments of the three anonymous reviewers and the Editor-in-Chief, Dr. Gottgens. NR 92 TC 4 Z9 4 U1 14 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD FEB PY 2016 VL 24 IS 1 BP 73 EP 92 DI 10.1007/s11273-015-9452-0 PG 20 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DD8IO UT WOS:000370170100007 ER PT J AU Khalil, S Ali, TA Skory, C Slininger, PJ Schisler, DA AF Khalil, Sadia Ali, Tasneem Adam Skory, Chris Slininger, Patricia J. Schisler, David A. TI Evaluation of economically feasible, natural plant extract-based microbiological media for producing biomass of the dry rot biocontrol strain Pseudomonas fluorescens P22Y05 in liquid culture SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Microbioreactor; Plant extract-based media; Biological control; Dry rot of potato ID POSTHARVEST DISEASES; BIOLOGICAL-CONTROL; MICROTITER PLATES; WATER ACTIVITY; GROWTH; POTATOES; EFFICACY; PH; TEMPERATURE; PERFORMANCE AB The production of microbial biomass in liquid media often represents an indispensable step in the research and development of bacterial and fungal strains. Costs of commercially prepared nutrient media or purified media components, however, can represent a significant hurdle to conducting research in locations where obtaining these products is difficult. A less expensive option for providing components essential to microbial growth in liquid culture is the use of extracts of fresh or dried plant products obtained by using hot water extraction techniques. A total of 13 plant extract-based media were prepared from a variety of plant fruits, pods or seeds of plant species including Allium cepa (red onion bulb), Phaseolus vulgaris (green bean pods), and Lens culinaris (lentil seeds). In shake flask tests, cell production by potato dry rot antagonist Pseudomonas fluorescens P22Y05 in plant extract-based media was generally statistically indistinguishable from that in commercially produced tryptic soy broth and nutrient broth as measured by optical density and colony forming units/ml produced (P <= 0.05, Fisher's protected LSD). The efficacy of biomass produced in the best plant extract-based media or commercial media was equivalent in reducing Fusarium dry rot by 50-96 % compared to controls. In studies using a high-throughput microbioreactor, logarithmic growth of P22Y05 in plant extract-based media initiated in 3-5 h in most cases but specific growth rate and the time of maximum OD varied as did the maximum pH obtained in media. Nutrient analysis of selected media before and after cell growth indicated that nitrogen in the form of NH4 accumulated in culture supernatants, possibly due to unbalanced growth conditions brought on by a scarcity of simple sugars in the media tested. The potential of plant extract-based media to economically produce biomass of microbes active in reducing plant disease is considerable and deserves further research. C1 [Khalil, Sadia; Ali, Tasneem Adam] Univ Karachi, Dept Microbiol, Karachi 75270, Pakistan. [Skory, Chris; Slininger, Patricia J.; Schisler, David A.] USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Schisler, DA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM David.Schisler@ars.usda.gov FU Higher Education Commission (HEC), Pakistan FX The assistance of Maggie Hammar and Kristina Glenzinski in conducting experiments is greatly appreciated. Studies were made possible, in part, by funding and travel support provided by the Higher Education Commission (HEC), Pakistan. NR 34 TC 0 Z9 0 U1 2 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD FEB PY 2016 VL 32 IS 2 AR 25 DI 10.1007/s11274-015-1984-1 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DD8HX UT WOS:000370168200008 PM 26745985 ER PT J AU Liu, CK Lyass, A Larson, MG Massaro, JM Wang, N D'Agostino, RB Benjamin, EJ Murabito, JM AF Liu, Christine K. Lyass, Asya Larson, Martin G. Massaro, Joseph M. Wang, Na D'Agostino, Ralph B., Sr. Benjamin, Emelia J. Murabito, Joanne M. TI Biomarkers of oxidative stress are associated with frailty: the Framingham Offspring Study SO AGE LA English DT Article DE Frailty; Inflammation; Oxidative stress; Gait speed ID PERIPHERAL ARTERIAL-DISEASE; C-REACTIVE PROTEIN; OLDER-ADULTS; WOMENS HEALTH; GAIT SPEED; CARDIOVASCULAR-DISEASE; MOBILITY DISABILITY; PHYSICAL-ACTIVITY; GRIP STRENGTH; VITAMIN-D AB Cardiovascular disease and frailty frequently occur together. Both are associated with inflammation, which may be partially triggered by oxidative stress, especially in cardiovascular disease. We investigated whether inflammatory and oxidative stress biomarkers linked to cardiovascular disease were associated with frailty and the related outcome of gait speed. We report cross-sectional associations of biomarkers and frailty assessed at Framingham Offspring Study cycle eight. Participants >= 60 years were eligible if they had information on frailty and at least one of the following: C-reactive protein, interleukin-6, tumor necrosis factor receptor 2, 8-epi-FGF alpha isoprostanes (isoprostanes), lipoprotein phospholipase A2 (LpPLA2) mass or activity, osteoprotegerin, intracellular adhesion molecule-1, monocyte chemoattractant protein-1 or P-selectin. Stepwise logistic models were utilized for frailty and stepwise linear models for gait speed. Covariates included age, sex, body mass index, smoking, and co-morbidities. Odds ratios (ORs) and slope estimates (B) are reported per standard deviation increase of loge-transformed biomarker. Of the 1919 participants, 142 (7 %) were frail. In a stepwise model, frailty odds increased with higher interleukin-6 (OR 1.90, 95 % CI 1.51, 2.38), isoprostanes (OR 1.46, 95% CI 1.12, 1.92), and LpPLA2 mass (OR 1.29, 95 % CI 1.00, 1.65). Stepwise regression found that slower gait speeds were associated with interleukin-6 (B=-0.025 m/s, 95 % CI 0.04, -0.01), isoprostanes (B=-0.019, 95 % CI -0.03, -0.008), LpPLA2 mass (B=-0.016, 95 % CI -0.03, -0.004), and osteoprotegerin (B = -0.015, 95 % CI -0.03, -0.002, all p< 0.05). Interleukin-6, isoprostanes, and LpPLA2 mass were associated with greater frailty odds and slower gait speeds. Oxidative stress may be a mechanism contributing to frailty. C1 [Liu, Christine K.] Boston Univ, Sch Med, Dept Med, Sect Geriatr, 88 E Newton St,Robinson 2, Boston, MA 02118 USA. [Liu, Christine K.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lyass, Asya; Larson, Martin G.; Massaro, Joseph M.; D'Agostino, Ralph B., Sr.; Benjamin, Emelia J.; Murabito, Joanne M.] Natl Heart Lung & Blood Inst Framingham Heart Stu, 73 Mt Wayte Ave,Suite 2, Framingham, MA 01702 USA. [Lyass, Asya; Larson, Martin G.; Wang, Na; D'Agostino, Ralph B., Sr.] Boston Univ, Dept Math & Stat, Boston, MA 02215 USA. [Larson, Martin G.; Massaro, Joseph M.] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. [Benjamin, Emelia J.] Boston Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA USA. [Benjamin, Emelia J.] Boston Univ, Sch Med, Dept Med, Sect Cardiovasc, Boston, MA 02118 USA. [Benjamin, Emelia J.] Boston Univ, Sch Med, Dept Med, Sect Prevent Med, Boston, MA 02118 USA. [Murabito, Joanne M.] Boston Univ, Sch Med, Dept Med, Gen Internal Med Sect, Boston, MA 02118 USA. RP Liu, CK (reprint author), Boston Univ, Sch Med, Dept Med, Sect Geriatr, 88 E Newton St,Robinson 2, Boston, MA 02118 USA.; Murabito, JM (reprint author), Natl Heart Lung & Blood Inst Framingham Heart Stu, 73 Mt Wayte Ave,Suite 2, Framingham, MA 01702 USA. EM Christine.Liu@bmc.org; murabito@bu.edu FU National Institute on Aging at the National Institutes of Health [R01-AG029451, 1R01-AG028321]; National Heart, Lung and Blood Institute at the National Institutes of Health [1RO1-HL64753, R01-HL076784]; National Heart, Lung, and Blood Institute; Boston University [N01-HC-25195]; American Federation for Aging Research; John A. Hartford Foundation; U.S. Department of Agriculture [1950-51000-068-01S] FX This work was supported by the National Institute on Aging (grant number R01-AG029451 to JM Murabito, 1R01-AG028321 to EJB) and by the National Heart, Lung and Blood Institute (grant number 1RO1-HL64753, R01-HL076784 to EJB), both at the National Institutes of Health. The Framingham Heart Study is supported by the National Heart, Lung, and Blood Institute in collaboration with Boston University (contract number N01-HC-25195). CKL was supported by the American Federation for Aging Research, the John A. Hartford Foundation, and the U.S. Department of Agriculture, under Agreement no. 1950-51000-068-01S. NR 56 TC 8 Z9 8 U1 5 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0161-9152 EI 1574-4647 J9 AGE JI Age PD FEB PY 2016 VL 38 IS 1 AR 1 DI 10.1007/s11357-015-9864-z PG 10 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA DD1PA UT WOS:000369692400001 PM 26695510 ER PT J AU Boddu, VM Brenner, MW AF Boddu, Veera M. Brenner, Matthew W. TI Energy dissipation in intercalated carbon nanotube forests with metal layers SO APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING LA English DT Article ID MECHANICAL-PROPERTIES; COMPOSITES; GROWTH; ARRAYS; WIRES; TIPS AB Vertically aligned carbon nanotube (CNT) forests were synthesized to study their quasi-static mechanical properties in a layered configuration with metallization. The top and bottom surfaces of CNT forests were metalized with Ag, Fe, and In using paste, sputtering, and thermal evaporation, respectively. Stacks of one, two, and three layers of these forests were assembled and compressed to measure their mechanical properties. The samples were strain limited to 0.7, and the results indicate that energy dissipation is approximately linear with respect to the number of layers and relatively independent of metal type. The energy per unit volume was approximately the same for all samples. Successive stacking of CNT forests reduces local buckling events, which is enhanced with a thick Ag deposition on the CNT forest surface. Young's modulus was also observed to increase as the number of layers was increased. These results are useful in the design of composite materials for high energy absorption and high stiffness applications. C1 [Boddu, Veera M.; Brenner, Matthew W.] US Army Engn Res & Dev Ctr, Construct Engn Res Lab ERDC CERL, Environm Proc Branch, Champaign, IL 61826 USA. [Boddu, Veera M.] ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Boddu, VM (reprint author), US Army Engn Res & Dev Ctr, Construct Engn Res Lab ERDC CERL, Environm Proc Branch, Champaign, IL 61826 USA.; Boddu, VM (reprint author), ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Veera.Boddu@ars.usda.gov NR 34 TC 0 Z9 0 U1 3 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0947-8396 EI 1432-0630 J9 APPL PHYS A-MATER JI Appl. Phys. A-Mater. Sci. Process. PD FEB PY 2016 VL 122 IS 2 AR 88 DI 10.1007/s00339-015-9571-8 PG 11 WC Materials Science, Multidisciplinary; Physics, Applied SC Materials Science; Physics GA DD5OB UT WOS:000369972400043 ER PT J AU Kimball, BA Stelting, SA McAuliffe, TW Stahl, RS Garcia, RA Pitt, WC AF Kimball, Bruce A. Stelting, Scott A. McAuliffe, Thomas W. Stahl, Randal S. Garcia, Rafael A. Pitt, William C. TI Development of artificial bait for brown treesnake suppression SO BIOLOGICAL INVASIONS LA English DT Article DE Boiga irregularis; Interdiction; Invasive; Pacific islands; Vertebrate ID GARTER SNAKES; SIGNAL-TRANSDUCTION; VOMERONASAL SYSTEM; RECEPTOR-BINDING; ISLAND; CHEMOATTRACTANT; AVERSION; GUAM AB The brown treesnake (Boiga irregularis) was accidentally introduced to Guam in the 1940s from the Admiralty Islands. A native of Australia, Papua New Guinea, and the Solomon Islands, the brown treesnake (BTS) continues to threaten the economy and ecology of Guam and is currently the subject of a cooperative program to control snake populations on the island and prevent its spread throughout the Pacific Rim. Delivery of toxic baits is a primary component of population suppression efforts. While many food items tested as baits for toxicant delivery provide relevant food prey cues leading to investigatory behaviors in BTS, only a few items tested in the past two decades have adequately promoted reliable consumption. Chief among them is the dead neonatal mouse (DNM). A series of chemical and bioassays were performed to identify materials with similar sensory qualities as DNM. Among the many items tested in a series of field experiments with free-ranging BTS in Guam, a processed meat product treated with an artificial mouse fat mixture was found to be removed from bait stations at rates greater than previously tested DNM substitutes and approaching removal rates of DNM. Furthermore, the test baits demonstrated excellent durability under field conditions. Further development of this bait offers great potential to satisfy many desirable attributes for BTS baiting operations. C1 [Kimball, Bruce A.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Monell Chem Senses Ctr, Philadelphia, PA 19104 USA. [Stelting, Scott A.; Stahl, Randal S.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [McAuliffe, Thomas W.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Hilo, HI 96720 USA. [Garcia, Rafael A.] ARS, USDA, Eastern Reg Res Ctr, Biobased & Other Anim Coprod Res Unit, Wyndmoor, PA 19038 USA. [Pitt, William C.] Smithsonian Conservat Biol Inst, Front Royal, VA 22630 USA. RP Kimball, BA (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Monell Chem Senses Ctr, 3500 Market St, Philadelphia, PA 19104 USA. EM bruce.a.kimball@aphis.usda.gov FU U.S. Department of Interior Office of Insular Affairs [101580RU033]; U.S. Department of the Navy (MIPR) [N6274212MP00024] FX These studies were made possible through funding provided by the U.S. Department of Interior Office of Insular Affairs (Agreement 101580RU033) and U.S. Department of the Navy (MIPR# N6274212MP00024) and generous access to Andersen AFB. The assistance of USDA-APHIS-WS staff located on the island of Guam was greatly appreciated; especially Daniel S. Vice (1970-2014), Assistant State Director for the Hawaii/Guam Program, who dedicated much of his career to the control and interdiction of invasive species. Spam (R) is a registered trademark of Hormel Food Corporation (Austin, MN). ARASCO (R) is a registered trademark of DSM Nutritional Products (Columbia, MD). We thank DSM Nutritional Products for providing a complimentary sample of ARASCO (R). Mention of specific trade names does not constitute endorsement by the US Department of Agriculture. NR 20 TC 0 Z9 0 U1 9 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD FEB PY 2016 VL 18 IS 2 BP 359 EP 369 DI 10.1007/s10530-015-1031-z PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DD6WV UT WOS:000370066200005 ER PT J AU Hishinuma, SM Dallara, PL Yaghmour, MA Zerillo, MM Parker, CM Roubtsova, TV Nguyen, TL Tisserat, NA Bostock, RM Flint, ML Seybold, SJ AF Hishinuma, Stacy M. Dallara, Paul L. Yaghmour, Mohammad A. Zerillo, Marcelo M. Parker, Corwin M. Roubtsova, Tatiana V. Nguyen, Tivonne L. Tisserat, Ned A. Bostock, Richard M. Flint, Mary L. Seybold, Steven J. TI Wingnut (Juglandaceae) as a new generic host for Pityophthorus juglandis (Coleoptera: Curculionidae) and the thousand cankers disease pathogen, Geosmithia morbida (Ascomycota: Hypocreales) SO CANADIAN ENTOMOLOGIST LA English DT Article ID WALNUT TWIG BEETLE; BLACK-WALNUT; PHYLOGENY; SUSCEPTIBILITY; RANGE; NIGRA; WOOD AB The walnut twig beetle (WTB), Pityophthorus juglandis Blackman (Coleoptera: Curculionidae), vectors a fungus, Geosmithia morbida Kolarik, Freeland, Utley, and Tisserat (Ascomycota: Hypocreales), which colonises and kills the phloem of walnut and butternut trees, Juglans Linnaeus (Juglandaceae). Over the past two decades, this condition, known as thousand cankers disease (TCD), has led to the widespread mortality of Juglans species in the United States of America. Recently the beetle and pathogen were discovered on several Juglans species in northern Italy. Little is known about the extra-generic extent of host acceptability and suitability for the WTB. We report the occurrence of both the WTB and G. morbida in three species of wingnut, Pterocarya fraxinifolia Spach, Pterocarya rhoifolia Siebold and Zuccarini, and Pterocarya stenoptera de Candolle (Juglandaceae) growing in the United States Department of Agriculture-Agricultural Research Service, National Clonal Germplasm Repository collection in northern California (NCGR) and in the Los Angeles County Arboretum and Botanic Garden in southern California, United States of America. In two instances (once in P. stenoptera and once in P. fraxinifolia) teneral (i.e., brood) adult WTB emerged and were collected more than four months after infested branch sections had been collected in the field. Koch's postulates were satisfied with an isolate of G. morbida from P. stenoptera, confirming this fungus as the causal agent of TCD in this host. A survey of the 37 Pterocarya Kunth accessions at the NCGR revealed that 46% of the trees had WTB attacks and/or symptoms of G. morbida infection. The occurrence of other subcortical Coleoptera associated with Pterocarya and the first occurrence of the polyphagous shot hole borer, a species near Euwallacea fornicatus Eichhoff (Coleoptera: Curculionidae), in Juglans are also documented. C1 [Hishinuma, Stacy M.; Dallara, Paul L.; Parker, Corwin M.; Flint, Mary L.] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA. [Yaghmour, Mohammad A.; Roubtsova, Tatiana V.; Nguyen, Tivonne L.; Bostock, Richard M.] Univ Calif Davis, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA. [Zerillo, Marcelo M.; Tisserat, Ned A.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Plant Sci 1177, Ft Collins, CO 80523 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95616 USA. RP Seybold, SJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95616 USA. EM sjseybold@gmail.com RI zerillo, marcelo/M-4159-2016 OI zerillo, marcelo/0000-0001-9671-2894 FU United States Department of Agriculture; NIFA Specialty Crops Research Initiative; United States Department of Agriculture, Forest Service, Washington Office Forest Health Protection; United States Department of Agriculture, Animal and Plant Health Inspection Service, Center for Plant Health Science Technology [10-CA-11272172-055, 10-JV-11272172-092]; University of California Agriculture and Natural Resources Competitive Grant [11-1025]; United States Department of Agriculture, Forest Service Washington Office; United States Department of Agriculture, Animal and Plant Health Inspection Service FX The authors thank John Preece and Howard Garrison (United States Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository) and Charles A. Leslie (University of California, Davis, Department of Plant Sciences) for access to and support of our research in the National Clonal Germplasm Repository Juglans and Pterocarya collections. They thank J.E. Henrich for access to the Los Angeles County Arboretum Collection and K.J. Greby, D.R. Hodel, A. Liu, and L.M. Ohara for assistance with collecting infested branches. The authors are grateful to L.J. Nelson (United States Department of Agriculture, Forest Service, Pacific Southwest Research Station), and especially to O. Batuman (University of California, Davis, Department of Plant Pathology) for assistance with obtaining and translating Turkish scientific literature. They also thank M. Aradhya (United States Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository), C.A. Leslie, and G. McGranahan (University of California, Davis, Department of Plant Sciences, retired) for critical reviews of the manuscript. The authors thank D.E. Bright (Colorado State University) for identifying Hypothenemus and Euwallacea, and R.L. Westcott (Oregon Department of Agriculture) for identifying Chrysobothris and Dicerca. This work was supported by grants from the United States Department of Agriculture, NIFA Specialty Crops Research Initiative, the United States Department of Agriculture, Forest Service, Washington Office Forest Health Protection; and United States Department of Agriculture, Animal and Plant Health Inspection Service, Center for Plant Health Science Technology administered through cooperative agreements 10-CA-11272172-055 and 10-JV-11272172-092 between the United States Department of Agriculture, Forest Service, Pacific Southwest Research Station and the University of California, Davis, Department of Entomology and Nematology. The work was also supported by University of California Agriculture and Natural Resources Competitive Grant number 11-1025 to R.M.B. and S.J.S. The authors are especially grateful to Bruce Moltzan for facilitating the funding from the United States Department of Agriculture, Forest Service Washington Office and to David Lance for facilitating the funding from United States Department of Agriculture, Animal and Plant Health Inspection Service. NR 40 TC 4 Z9 4 U1 14 U2 18 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD FEB PY 2016 VL 148 IS 1 BP 83 EP 91 DI 10.4039/tce.2015.37 PG 9 WC Entomology SC Entomology GA DD0DT UT WOS:000369591200008 ER PT J AU Belval, EJ Wei, Y Bevers, M AF Belval, Erin J. Wei, Yu Bevers, Michael TI A stochastic mixed integer program to model spatial wildfire behavior and suppression placement decisions with uncertain weather SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE multistage stochastic programming; wildfire growth; wildfire suppression; uncertain fire weather ID INITIAL ATTACK RESOURCES; STANDARD RESPONSE MODEL; FIRE MANAGEMENT; RISK AB Wildfire behavior is a complex and stochastic phenomenon that can present unique tactical management challenges. This paper investigates a multistage stochastic mixed integer program with full recourse to model spatially explicit fire behavior and to select suppression locations for a wildland fire. Simplified suppression decisions take the form of "suppression nodes", which are placed on a raster landscape for multiple decision stages. Weather scenarios are used to represent a distribution of probable changes in fire behavior in response to random weather changes, modeled using probabilistic weather trees. Multistage suppression decisions and fire behavior respond to these weather events and to each other. Nonanticipativity constraints ensure that suppression decisions account for uncertainty in weather forecasts. Test cases for this model provide examples of fire behavior interacting with suppression to achieve a minimum expected area impacted by fire and suppression. C1 [Belval, Erin J.; Wei, Yu] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. [Bevers, Michael] USDA, Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Belval, EJ (reprint author), Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. EM mccowene@gmail.com FU USDA Forest Service Rocky Mountain Research Station [11-JV-11221636-146]; Colorado State University [11-JV-11221636-146] FX This research was supported by Joint Venture Agreement 11-JV-11221636-146 between the USDA Forest Service Rocky Mountain Research Station and Colorado State University. The authors thank the associate editor and the referees whose insightful suggestions significantly improved this paper. NR 29 TC 0 Z9 0 U1 6 U2 8 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD FEB PY 2016 VL 46 IS 2 BP 234 EP 248 DI 10.1139/cjfr-2015-0289 PG 15 WC Forestry SC Forestry GA DD6QU UT WOS:000370049600010 ER PT J AU Caouette, JP Steel, EA Hennon, PE Cunningham, PG Pohl, CA Schrader, BA AF Caouette, J. P. Steel, E. A. Hennon, P. E. Cunningham, P. G. Pohl, C. A. Schrader, B. A. TI Influence of elevation and site productivity on conifer distributions across Alaskan temperate rainforests SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE landscape; elevation; species distribution models; climate; biogeography ID CLIMATE-CHANGE; SPECIES DISTRIBUTION; SOUTHEAST ALASKA; UNITED-STATES; TREE; DYNAMICS; MODELS; MORTALITY; MIGRATION; CONSERVATION AB We investigated the influence of landscape factors on the distribution and life stage stability of coastal tree species near the northern limit of their ranges. Using data from 1465 forest inventory plots, we estimated probability of occurrence and basal area of six common conifer species across three broad latitudinal regions of coastal Alaska. By also comparing models across life stages of each species (seedlings, saplings, mature trees, and dead trees), we explored trends in population stability at this leading edge of climate change. Elevation had a stronger influence on the probability of tree species occurrence than on basal area; site productivity impacted both estimated odds of presence and estimated basal area for most species in at least some regions. Interestingly, there were fairly dramatic differences across species in the degree to which the modeled probability of occurrence differed across the four life stages. Western redcedar (Thuja plicata Donn ex D. Don), for example, showed relatively stable distributions but other species appear to be in flux, e.g., yellow-cedar (Callitropsis nootkatensis (D. Don) D. P. Little), which has experienced widespread mortality at low elevations. Differential effects of elevation on live versus dead basal area suggest that mountain hemlock (Tsuga mertensiana (Bong.) Carriere) and yellow-cedar are shifting upslope in some regions and that Sitka spruce (Picea sitchensis (Bong.) Carriere) is shifting downslope in the Northwest region. C1 [Caouette, J. P.] Nature Conservancy, 416 Harris St 301, Juneau, AK USA. [Steel, E. A.] USDA, Forest Serv, Pacific NW Res Stn, 400 N 34th St, Seattle, WA 98103 USA. [Hennon, P. E.] USDA, Forest Serv, Pacific NW Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. [Cunningham, P. G.] USDA, Forest Serv, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Pohl, C. A.] Catherine Pohl Biol Consulting, POB 21168, Juneau, AK 99802 USA. [Schrader, B. A.] USDA, Forest Serv, Alaska Reg, 709 W 9th St, Juneau, AK 99801 USA. RP Hennon, PE (reprint author), USDA, Forest Serv, Pacific NW Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. EM phennon@fs.fed.us FU USFS Region 10 Regional Office; Nature Conservancy FX We wish to thank Tara Barrett and Ken Winterburger for guidance with forest inventory plot data, Rob Develice and Robert Pattison for help in interpreting the northwest limits of tree species, Melinda Lamb who produced Fig. 1, and Tara Barrett and Lauren Oakes who made helpful suggestions on an early manuscript draft. We appreciate the financial assistance from the USFS Region 10 Regional Office and oversight by The Nature Conservancy for their support to John Caouette. NR 49 TC 0 Z9 0 U1 4 U2 14 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD FEB PY 2016 VL 46 IS 2 BP 249 EP 261 DI 10.1139/cjfr-2015-0283 PG 13 WC Forestry SC Forestry GA DD6QU UT WOS:000370049600011 ER PT J AU Mullan, D Chen, J Zhang, XJ AF Mullan, Donal Chen, Jie Zhang, Xunchang John TI Validation of non-stationary precipitation series for site-specific impact assessment: comparison of two statistical downscaling techniques SO CLIMATE DYNAMICS LA English DT Article DE Climate impacts; Statistical downscaling; Precipitation; Non-stationary series; Validation ID GENERAL-CIRCULATION MODEL; CLIMATE-CHANGE SCENARIOS; NORTHWESTERN UNITED-STATES; SOIL-EROSION; LOCAL CLIMATE; GCM OUTPUT; NASA/DAO REANALYSES; WEATHER GENERATOR; NORTHERN-IRELAND; REGIONAL SCALES AB Statistical downscaling (SD) methods have become a popular, low-cost and accessible means of bridging the gap between the coarse spatial resolution at which climate models output climate scenarios and the finer spatial scale at which impact modellers require these scenarios, with various different SD techniques used for a wide range of applications across the world. This paper compares the Generator for Point Climate Change (GPCC) model and the Statistical DownScaling Model (SDSM)-two contrasting SD methods-in terms of their ability to generate precipitation series under non-stationary conditions across ten contrasting global climates. The mean, maximum and a selection of distribution statistics as well as the cumulative frequencies of dry and wet spells for four different temporal resolutions were compared between the models and the observed series for a validation period. Results indicate that both methods can generate daily precipitation series that generally closely mirror observed series for a wide range of non-stationary climates. However, GPCC tends to overestimate higher precipitation amounts, whilst SDSM tends to underestimate these. This infers that GPCC is more likely to overestimate the effects of precipitation on a given impact sector, whilst SDSM is likely to underestimate the effects. GPCC performs better than SDSM in reproducing wet and dry day frequency, which is a key advantage for many impact sectors. Overall, the mixed performance of the two methods illustrates the importance of users performing a thorough validation in order to determine the influence of simulated precipitation on their chosen impact sector. C1 [Mullan, Donal] Queens Univ Belfast, Sch Geog Archaeol & Palaeoecol, Elmwood Ave, Belfast BT7 1NN, Antrim, North Ireland. [Chen, Jie] Univ Quebec, Ecole Technol Super, Dept Construct Engn, Montreal, PQ H3C 3P8, Canada. [Zhang, Xunchang John] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. RP Mullan, D (reprint author), Queens Univ Belfast, Sch Geog Archaeol & Palaeoecol, Elmwood Ave, Belfast BT7 1NN, Antrim, North Ireland. EM D.Mullan@qub.ac.uk NR 79 TC 1 Z9 1 U1 6 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0930-7575 EI 1432-0894 J9 CLIM DYNAM JI Clim. Dyn. PD FEB PY 2016 VL 46 IS 3-4 BP 967 EP 986 DI 10.1007/s00382-015-2626-x PG 20 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DD6JQ UT WOS:000370030900018 ER PT J AU McKelvey, KS Young, MK Wilcox, TM Bingham, DM Pilgrim, KL Schwartz, MK AF McKelvey, Kevin S. Young, Michael K. Wilcox, Taylor M. Bingham, Daniel M. Pilgrim, Kristine L. Schwartz, Michael K. TI Patterns of hybridization among cutthroat trout and rainbow trout in northern Rocky Mountain streams SO ECOLOGY AND EVOLUTION LA English DT Article DE cutthroat; hybridization; Oncorhynchus; SNP; swarm ID ONCORHYNCHUS-CLARKII-LEWISI; ENDANGERED SPECIES ACT; SOUTHEASTERN BRITISH-COLUMBIA; HYBRID ZONE STRUCTURE; WESTSLOPE CUTTHROAT; INTROGRESSIVE HYBRIDIZATION; GENETIC IDENTIFICATION; SPAWNING HABITAT; RIVER DRAINAGE; SALMO-CLARKI AB Introgressive hybridization between native and introduced species is a growing conservation concern. For native cutthroat trout and introduced rainbow trout in western North America, this process is thought to lead to the formation of hybrid swarms and the loss of monophyletic evolutionary lineages. Previous studies of this phenomenon, however, indicated that hybrid swarms were rare except when native and introduced forms of cutthroat trout co-occurred. We used a panel of 86 diagnostic, single nucleotide polymorphisms to evaluate the genetic composition of 3865 fish captured in 188 locations on 129 streams distributed across western Montana and northern Idaho. Although introgression was common and only 37% of the sites were occupied solely by parental westslope cutthroat trout, levels of hybridization were generally low. Of the 188 sites sampled, 73% contained 5% rainbow trout alleles and 58% had 1% rainbow trout alleles. Overall, 72% of specimens were nonadmixed westslope cutthroat trout, and an additional 3.5% were nonadmixed rainbow trout. Samples from seven sites met our criteria for hybrid swarms, that is, an absence of nonadmixed individuals and a random distribution of alleles within the sample; most (6/7) were associated with introgression by Yellowstone cutthroat trout. In streams with multiple sites, upstream locations exhibited less introgression than downstream locations. We conclude that although the widespread introduction of nonnative trout within the historical range of westslope cutthroat trout has increased the incidence of introgression, sites containing nonadmixed populations of this taxon are common and broadly distributed. C1 [McKelvey, Kevin S.; Young, Michael K.; Wilcox, Taylor M.; Pilgrim, Kristine L.; Schwartz, Michael K.] USDA Forest Serv, Rocky Mt Res Stn, Natl Genom Ctr Wildlife & Fish Conservat, 800 East Beckwith Ave, Missoula, MT 59801 USA. [Wilcox, Taylor M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Bingham, Daniel M.] Rogue Biol Consultants, 215 NW 22nd Pl Suite 207, Portland, OR 97217 USA. RP McKelvey, KS (reprint author), USDA Forest Serv, Rocky Mt Res Stn, Natl Genom Ctr Wildlife & Fish Conservat, 800 East Beckwith Ave, Missoula, MT 59801 USA. EM kmckelvey@fs.fed.us FU USFS Rocky Mountain Research Station, Region 1; Washington Office Fish and Aquatic Ecology Unit FX Funding for this project was provided by the USFS Rocky Mountain Research Station, Region 1, and the Washington Office Fish and Aquatic Ecology Unit. NR 122 TC 3 Z9 3 U1 17 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD FEB PY 2016 VL 6 IS 3 BP 688 EP 706 DI 10.1002/ece3.1887 PG 19 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DD5OY UT WOS:000369974900005 PM 26865958 ER PT J AU Carvalho, G Schaffert, RE Malosetti, M Viana, JHM Menezes, CB Silva, LA Guimaraes, CT Coelho, AM Kochian, LV van Eeuwijk, FA Magalhaes, JV AF Carvalho, Geraldo, Jr. Schaffert, Robert Eugene Malosetti, Marcos Moreira Viana, Joao Herbert Menezes, Cicero Bezerra Silva, Lidianne Assis Guimaraes, Claudia Teixeira Coelho, Antonio Marcos Kochian, Leon V. van Eeuwijk, Fred A. Magalhaes, Jurandir Vieira TI Back to Acid Soil Fields: The Citrate Transporter SbMATE Is a Major Asset for Sustainable Grain Yield for Sorghum Cultivated on Acid Soils SO G3-GENES GENOMES GENETICS LA English DT Article DE Al tolerance; QTL mapping; Sorghum bicolor; Alt(SB); field trials ID QUANTITATIVE TRAIT LOCI; ALUMINUM-TOLERANCE GENE; MALATE TRANSPORTERS; ACTIVATED CITRATE; DROUGHT TOLERANCE; NUTRIENT SOLUTION; STRESS TRIALS; ORGANIC-ACIDS; MIXED-MODEL; WEST-AFRICA AB Aluminum (Al) toxicity damages plant roots and limits crop production on acid soils, which comprise up to 50% of the world's arable lands. A major Al tolerance locus on chromosome 3, Alt(SB), controls aluminum tolerance in sorghum [Sorghum bicolor (L.) Moench] via SbMATE, an Al-activated plasma membrane transporter that mediates Al exclusion from sensitive regions in the root apex. As is the case with other known Al tolerance genes, SbMATE was cloned based on studies conducted under controlled environmental conditions, in nutrient solution. Therefore, its impact on grain yield on acid soils remains undetermined. To determine the real world impact of SbMATE, multi-trait quantitative trait loci (QTL) mapping in hydroponics, and, in the field, revealed a large-effect QTL colocalized with the Al tolerance locus AltSB, where SbMATE lies, conferring a 0.6 ton ha(-1) grain yield increase on acid soils. A second QTL for Al tolerance in hydroponics, where the positive allele was also donated by the Al tolerant parent, SC283, was found on chromosome 9, indicating the presence of distinct Al tolerance genes in the sorghum genome, or genes acting in the SbMATE pathway leading to Al-activated citrate release. There was no yield penalty for AltSB, consistent with the highly localized Al regulated SbMATE expression in the root tip, and Al-dependent transport activity. A female effect of 0.5 ton ha(-1) independently demonstrated the effectiveness of AltSB in hybrids. Al tolerance conferred by AltSB is thus an indispensable asset for sorghum production and food security on acid soils, many of which are located in developing countries. C1 [Carvalho, Geraldo, Jr.; Schaffert, Robert Eugene; Moreira Viana, Joao Herbert; Menezes, Cicero Bezerra; Silva, Lidianne Assis; Guimaraes, Claudia Teixeira; Coelho, Antonio Marcos; Magalhaes, Jurandir Vieira] Embrapa Maize & Sorghum, Rod MG 424,Km 45, BR-35701970 Sete Lagoas, MG, Brazil. [Malosetti, Marcos; van Eeuwijk, Fred A.] Univ Wageningen & Res Ctr, Biometris, NL-6700 AC Wageningen, Netherlands. [Kochian, Leon V.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Silva, Lidianne Assis] Univ Fed Acre, Dept Plant Breeding & Genet, BR-69915900 Cruzeiro Do Sul, AC, Brazil. RP Magalhaes, JV (reprint author), Embrapa Maize & Sorghum, Rod MG 424,Km 45, BR-35701970 Sete Lagoas, MG, Brazil. EM jurandir.magalhaes@embrapa.br FU CGIAR Generation Challenge Program [G3007.04]; Embrapa Macroprogram; McKnight Foundation Collaborative Crop Research Program; National Council for Scientific and Technological Development (CNPq) FX The authors acknowledge funding from the CGIAR Generation Challenge Program (project G3007.04), the Embrapa Macroprogram, and the McKnight Foundation Collaborative Crop Research Program. The authors are also thankful to The National Council for Scientific and Technological Development (CNPq) for an MS fellowship granted to G.C., and for support to J.V.M. NR 65 TC 0 Z9 0 U1 6 U2 14 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD FEB 1 PY 2016 VL 6 IS 2 BP 475 EP 484 DI 10.1534/g3.115.025791 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA DD0FH UT WOS:000369595300022 ER PT J AU Coates, BS Alves, AP Wang, H Zhou, X Nowatzki, T Chen, H Rangasamy, M Robertson, HM Whitfield, CW Walden, KK Kachman, SD French, BW Meinke, LJ Hawthorne, D Abel, CA Sappington, TW Siegfried, BD Miller, NJ AF Coates, B. S. Alves, A. P. Wang, H. Zhou, X. Nowatzki, T. Chen, H. Rangasamy, M. Robertson, H. M. Whitfield, C. W. Walden, K. K. Kachman, S. D. French, B. W. Meinke, L. J. Hawthorne, D. Abel, C. A. Sappington, T. W. Siegfried, B. D. Miller, N. J. TI Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera SO INSECT MOLECULAR BIOLOGY LA English DT Article DE pesticide resistance; methyl-parathion resistance; genome mapping; functional genomics ID METHYL-PARATHION RESISTANCE; CHRYSOMELIDAE LARVAL INJURY; MOSQUITO AEDES-AEGYPTI; INSECTICIDE RESISTANCE; GENE-EXPRESSION; POPULATIONS COLEOPTERA; MICROSATELLITE MARKERS; MICROARRAY ANALYSIS; DNA MICROARRAY; CELL-CYCLE AB The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases >= 2.43) being an alpha-esterase-like transcript. Differential expression was validated only for the alpha-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methylparathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyx-esterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established. C1 [Coates, B. S.; Abel, C. A.; Sappington, T. W.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Alves, A. P.; Wang, H.; Zhou, X.; Nowatzki, T.; Chen, H.; Rangasamy, M.; Kachman, S. D.; Meinke, L. J.; Siegfried, B. D.; Miller, N. J.] Univ Nebraska, Lincoln, NE USA. [Robertson, H. M.; Whitfield, C. W.; Walden, K. K.] Univ Illinois, Champaign, IL 61820 USA. [French, B. W.] USDA ARS, North Cent Agr Res Lab, Brooking, SD USA. [Hawthorne, D.] Univ Maryland, College Pk, MD 20742 USA. [Abel, C. A.; Sappington, T. W.] Iowa State Univ, Ames, IA USA. RP Coates, BS (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA.; Coates, BS (reprint author), Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res, 113 Genet Lab, Ames, IA 50010 USA. EM brad.coates@ars.udsa.gov OI Kachman, Stephen/0000-0003-0506-513X FU USDA-ARS [Current Research Information System (CRIS)] [3625-22000-017-00, 5447-21220-005-00D]; Iowa Agriculture and Home Economics Experiment Station, Ames, IA [3543]; University of Nebraska; USDA, National Institute of Food and Agriculture [2009-05246] FX This research was a joint contribution from the USDA-ARS [Current Research Information System (CRIS) projects 3625-22000-017-00 and 5447-21220-005-00D], the Iowa Agriculture and Home Economics Experiment Station, Ames, IA (project 3543) and the University of Nebraska. Funding was also provided by a USDA, National Institute of Food and Agriculture grant (award number 2009-05246). This article reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture, Iowa State University or the University of Nebraska for their use. We thank USDA technicians Bob Gunnarson for assisting with PCR screening, and Chad Nielson for help in generating the pedigrees. USDA is an equal opportunity provider and employer. NR 88 TC 1 Z9 1 U1 4 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD FEB PY 2016 VL 25 IS 1 BP 1 EP 15 DI 10.1111/imb.12194 PG 15 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA DD5QH UT WOS:000369978600001 PM 26566705 ER PT J AU Nam, WH Hong, EM Baigorria, GA AF Nam, Won-Ho Hong, Eun-Mi Baigorria, Guillermo A. TI How climate change has affected the spatio-temporal patterns of precipitation and temperature at various time scales in North Korea SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article DE climate change; spatial structure; correlation matrix; precipitation; temperature; North Korea ID TEMPORAL TRENDS; VARIABILITY; REGIME AB Detecting changes in the spatio-temporal patterns of temperature and precipitation is a prerequisite for developing effective adaptation options and strategies for the future. An effective method for assessing climate change and for providing information to decision makers and stakeholders is needed to implement appropriate adaptation strategies. The objective of this study was to determine whether climate change has caused spatio-temporal changes in meteorological elements in North Korea. We delineated the spatio-temporal patterns of temperature and precipitation caused by climate change in specific time periods based on statistically significant differences using a statistically robust method. Historical weather data from 27 meteorological stations over a 30-year period (1981-2010) were used. The results demonstrated that statistically significant changes occurred over the 30 years. The temporal trends in the maximum and minimum temperatures were highly significantly different in the western agricultural regions and central/southwest urban regions during 1996-2010 compared with 1981-1995. The precipitation amounts were significantly different in the southeast regions (around the coast). The numbers of precipitation events were significantly different for portions of the northern and northeast areas near the mountains. Additionally, statistically significant differences in the spatial structures of the temperature and precipitation were found at different time scales. The significant differences were not uniform in each season/month. Therefore, significant differences occurred in the meteorological elements, and particular locations and urbanized areas were affected by global warming. However, the temporal trends and spatial structures of each meteorological element were not equally modified; the meteorological changes occurred locally as a result of the changing climate. C1 [Nam, Won-Ho] Univ Nebraska, Natl Drought Mitigat Ctr, Sch Nat Resources, Lincoln, NE 68583 USA. [Hong, Eun-Mi] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD USA. [Baigorria, Guillermo A.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Baigorria, Guillermo A.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. RP Baigorria, GA (reprint author), Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA.; Baigorria, GA (reprint author), Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. EM gbaigorria@unl.edu OI NAM, WONHO/0000-0002-9671-6569 FU Basic Science Research Program through the National Research Foundation of Korea (NRF); Ministry of Education, Science and Technology [2013R1A6A3 A03019009]; Robert B. Daugherty Water for Food Institute (WFI); CropClimate Initiative FX This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and was funded by the Ministry of Education, Science and Technology (2013R1A6A3 A03019009), and it was developed under the auspices of the Robert B. Daugherty Water for Food Institute (WFI), and the CropClimate Initiative. The authors wish to thank one anonymous reviewer and Hi-Ryong Byun for their valuable suggestions regarding the manuscript. The views expressed in this paper are those of the authors and do not necessarily reflect the views of NRF, WFI, or any of its sub-agencies. NR 26 TC 1 Z9 1 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 EI 1097-0088 J9 INT J CLIMATOL JI Int. J. Climatol. PD FEB PY 2016 VL 36 IS 2 BP 722 EP 734 DI 10.1002/joc.4378 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DD5VC UT WOS:000369991700014 ER PT J AU Hurry, J Risk, D Lavoie, M Brooks, BG Phillips, CL Gockede, M AF Hurry, Jacquelyn Risk, David Lavoie, Martin Brooks, Bjorn-Gustaf Phillips, Claire L. Goeckede, Mathias TI Atmospheric monitoring and detection of fugitive emissions for Enhanced Oil Recovery SO INTERNATIONAL JOURNAL OF GREENHOUSE GAS CONTROL LA English DT Article DE Carbon Capture Utilization and Storage; Enhanced Oil Recovery; Multi-gas; Atmospheric; Monitoring ID FLUID-ROCK INTERACTION; UNITED-STATES; CO2 STORAGE; WEYBURN; CANADA; SITE; SASKATCHEWAN; METHANE AB In Weyburn, Saskatchewan, carbon dioxide (CO2) is injected into the Weyburn oilfield for Enhanced Oil Recovery (EOR). Cenovus Energy Inc. operates more than 1000 active wells, processing plants, and hundreds of kilometres of pipeline infrastructure over a >100 km(2) area. While vehicle-based atmospheric detection of gas leakage would be convenient for a distributed operation such as Weyburn, implementing atmospheric detection schemes, particularly those that target CO2, are challenging in that natural ecosystems and other human activities both emit CO2 and will contribute to regular false positives. Here we present field test results of a multi-gas atmospheric detection technique that uses observed trace gas ratios (CO2, CH4, and H2S) to discriminate plumes of gas originating from different sources. This work is part of a larger project focused on multi-scale fugitive emissions detection and plume discrimination. During 2013 and 2014, we undertook vehicle-based mobile surveys of CO2, CH4, H2S, and delta(CH4)-C-13, in the Weyburn oilfield, using customized Cavity Ring Down Spectroscopy (CRDS) instruments that also alternated as stationary receptors. Mobile surveys provided georeferenced observations of atmospheric gas concentrations every 20-30 m, along a route driven at roughly 70 km h(-1). Data were uploaded to remote servers and processed using visualization tools that allowed us to constrain the location and timing of potential emission events. Results from one day of mobile surveying, September 24,2013, are presented here to illustrate how industrial activities, combustion engine and flare stack source emissions can be discriminated on the basis of excess mixing gas ratios, at distances from a few hundreds metres, to kilometres, in the Weyburn oilfield. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Hurry, Jacquelyn; Risk, David; Lavoie, Martin] St Francis Xavier Univ, Dept Earth Sci, Antigonish, NS B2G 1C0, Canada. [Brooks, Bjorn-Gustaf] USDA Forest Serv, South Res Stn, Asheville, NC USA. [Phillips, Claire L.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Goeckede, Mathias] Max Planck Inst Biogeochem, D-07745 Jena, Germany. RP Risk, D (reprint author), St Francis Xavier Univ, Dept Earth Sci, Antigonish, NS B2G 1C0, Canada. EM drisk@stfx.ca RI Goeckede, Mathias/C-1027-2017 OI Goeckede, Mathias/0000-0003-2833-8401 FU Natural Resources Canada Eco-Energy Initiative FX We would like to thank; Rae Lynn Spencer, Marc Dubord, and James Stirling of Cenovus Energy; Jocelyn Egan for the help with data sampling; and the Natural Resources Canada Eco-Energy Initiative for generous support of this work. NR 19 TC 1 Z9 1 U1 3 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1750-5836 EI 1878-0148 J9 INT J GREENH GAS CON JI Int. J. Greenh. Gas Control PD FEB PY 2016 VL 45 BP 1 EP 8 DI 10.1016/j.ijggc.2015.11.031 PG 8 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels; Engineering, Environmental SC Science & Technology - Other Topics; Energy & Fuels; Engineering GA DD7IN UT WOS:000370097000001 ER PT J AU Kim, S Adkins, J Aglan, HA Biswas, A Selling, G AF Kim, Sanghoon Adkins, Jason Aglan, Heshmat A. Biswas, Atanu Selling, Gordon TI Polymer composites prepared from heat-treated starch and styrene-butadiene latex SO JOURNAL OF ELASTOMERS AND PLASTICS LA English DT Article DE Starch; latex; polymer composite; tensile strength; hardness ID REINFORCED NATURAL-RUBBER; MECHANICAL-PROPERTIES; NANOCRYSTALS; FILLER AB Thermoplastic starch/latex polymer composites were prepared using styrene-butadiene latex and heat-treated cornstarch. The composites were prepared in a compression mold at 130 degrees C, with 20% starch content. An amylose-free cornstarch, waxy maize, was used for this research, and the heat treatment range was from ambient temperature to 170 degrees C. The heat-treated starch (HTS) was characterized by optical microscopy, dynamic light scattering, and size exclusion chromatography. The composites were characterized by mechanical analysis (stress-strain) and by scanning electron microscopy. The mechanical tests of composites revealed a dramatic increase in the modulus and tensile strength when the gelatinized starch was incorporated. When the starch was not gelatinized, starch did not contribute to the increase in the modulus and tensile strength because of its small surface area, thus weak interaction with the matrix. The reinforcing effect of starch as filler was reduced as the heating temperature of starch was increased to higher than 120 degrees C. The variation of reinforcement effect of HTS as filler was explained by the interaction between starch and matrix material, latex. C1 [Kim, Sanghoon; Adkins, Jason; Biswas, Atanu; Selling, Gordon] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Aglan, Heshmat A.] Tuskegee Univ, Dept Mech Engn, Tuskegee, AL 36088 USA. RP Kim, S (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM sanghoon.kim@ars.usda.gov NR 25 TC 1 Z9 1 U1 1 U2 4 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0095-2443 EI 1530-8006 J9 J ELASTOM PLAST JI J. Elastomer Plast. PD FEB PY 2016 VL 48 IS 1 BP 80 EP 93 DI 10.1177/0095244314538440 PG 14 WC Materials Science, Multidisciplinary; Polymer Science SC Materials Science; Polymer Science GA DD6UP UT WOS:000370060300004 ER PT J AU Hsieh, YC Poole, TL Runyon, M Hume, M Herrman, TJ AF Hsieh, Yi-Cheng Poole, Tom L. Runyon, Mick Hume, Michael Herrman, Timothy J. TI Prevalence of Nontyphoidal Salmonella and Salmonella Strains with Conjugative Antimicrobial-Resistant Serovars Contaminating Animal Feed in Texas SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Animal feed; Antimicrobial resistance; Feed ingredients; Preharvest food safety; Salmonella ID ESCHERICHIA-COLI; UNITED-STATES; MULTIDRUG-RESISTANT; FOODBORNE ILLNESS; DRUG-RESISTANCE; RISK-FACTORS; PLASMIDS; ENTERICA; SUSCEPTIBILITY; INGREDIENTS AB The objective of this study was to characterize 365 nontyphoidal Salmonella enterica isolates from animal feed. Among the 365 isolates, 78 serovars were identified. Twenty-four isolates (7.0%) were recovered from three of six medicated feed types. Three of these isolates derived from the medicated feed, Salmonella Newport, Salmonella Typhimurium var. O 5-(Copenhagen), and Salmonella Lexington var. 15+ (Manila), displayed antimicrobial resistance. Susceptibility testing revealed that only 3.0% (12) of the 365 isolates displayed resistance to any of the antimicrobial agents. These 12 isolates were recovered from unmedicated dry beef feed (n = 3), medicated dry beef feed (n = 3), cabbage culls (n = 2), animal protein products (n = 2), dry dairy cattle feed (n = 1), and fish meal (n = 1). Only Salmonella Newport and Salmonella Typhimurium var. O 5-(Copenhagen) were multidrug resistant. Both isolates possessed the IncA/C replicon and the bla(CMY-2) gene associated with cephalosporin resistance. Plasmid replicons were amplified from 4 of 12 resistant isolates. Plasmids (40 kb) were Salmonella Montevideo and Salmonella Kentucky. Conjugation experiments were done using 7 of the 12 resistant isolates as donors. Only Salmonella Montevideo, possessing a plasmid and amplifying IncN, produced transconjugants. Transconjugants displayed the same antimicrobial resistance profile as did the donor isolate. Three isolates that amplified replicons corresponding to IncA/C or IncHI2 did not produce transconjugants at 30 or 37 degrees C. The results of this study suggest that the prevalence of antimicrobial-resistant Salmonella contaminating animal feed is low in Texas. However, Salmonella was more prevalent in feed by-products; fish meal had the highest prevalence (84%) followed by animal protein products (48%). Ten of the 35 feed types had no Salmonella contamination. Further investigation is needed to understand the possible role of specific feed types in the dissemination of antimicrobial resistant bacteria. C1 [Hsieh, Yi-Cheng; Runyon, Mick; Herrman, Timothy J.] Texas A&M Univ Syst, Texas Agr Expt Stn, Off Texas State Chemist, College Stn, TX 77843 USA. [Poole, Tom L.; Hume, Michael] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Hsieh, YC (reprint author), Texas A&M Univ Syst, Texas Agr Expt Stn, Off Texas State Chemist, College Stn, TX 77843 USA. EM hsieh@otsc.tamu.edu NR 42 TC 0 Z9 0 U1 0 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2016 VL 79 IS 2 BP 194 EP 204 DI 10.4315/0362-028X.JFP-15-163 PG 11 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DD2TR UT WOS:000369775600002 PM 26818979 ER PT J AU Levent, G Harvey, RB Ciftcioglu, G Beier, RC Genovese, KJ He, HL Anderson, RC Nisbet, DJ AF Levent, G. Harvey, R. B. Ciftcioglu, G. Beier, R. C. Genovese, K. J. He, H. L. Anderson, R. C. Nisbet, D. J. TI In Vitro Effects of Thymol-beta-D-Glucopyranoside on Salmonella enterica Serovar Typhimurium and Escherichia coli K88 SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Escherichia coli; Intervention methods; Salmonella; beta-Thymol ID DIPHENYLIODONIUM CHLORIDE; ANTIMICROBIAL ACTIVITY; TRANS-CINNAMALDEHYDE; ESSENTIAL OILS; GUT BACTERIA; CAMPYLOBACTER; CARVACROL; OUTBREAK; EUGENOL; PIGLETS AB Although thymol is bactericidal against many pathogens in vitro, its in vivo effectiveness against pathogens in the lower gastrointestinal tract is limited because of its rapid absorption in the proximal gut. Thymol-beta-D-glucopyranoside (beta-thymol), a conjugated form of thymol, can deliver thymol to the lower gastrointestinal tract and has shown antibacterial effects. In the present study, we examined the in vitro effects of beta-thymol on Salmonella enterica serovar Typhimurium (ST) and Escherichia coli K88 (K88). We inoculated one-half strength Mueller-Hinton broth with 5.8 +/- 0.09 log CFU/ml novobiocin- and naladixic acid-resistant (NN) ST (NVSL 95-1776) and 5.1 +/- 0.09 log CFU ml(-1) NN-resistant K88, with or without porcine feces (0.1% [wt/vol]) (fecal incubations). The resultant bacterial suspensions were distributed under N-2 to triplicate sets of tubes to achieve initial concentrations of 0, 3, 6, and 12 mM for ST treatments and 0, 3, 12, and 30 mM for K88 treatments. Samples were incubated at 39 degrees C and then plated onto NN-containing brilliant green agar and NN-containing MacConkey agar; ST and K88 CFU concentrations were determined via 10-fold dilutions, and viable cell counts were performed at 0, 6, and 24 h. No differences in ST CFU counts were observed in beta-thymol treated tubes without the added porcine feces (i.e., pure culture) at 6 or 24 h. However, in tubes that contained fecal incubations, ST CFU counts were reduced (P < 0.05) from controls at 6 h in tubes treated with 6 and 12 mM beta-thymol, whereas in tubes treated with 3, 6, and 12 mM beta-thymol the CFU counts were reduced (P < 0.05) at 24 h. No differences were observed in K88 CFU counts in pure culture or in fecal incubations at 6 h, but K88 CFU counts were reduced (P < 0.05) in both pure and fecal incubations at 24 h. The results from this study demonstrate that beta-thymol, in the presence of fecal suspensions, has anti-Salmonella and anti-E. coli effects, suggesting a role of beta-glycoside-hydrolyzing microbes for the release of bactericidal thymol from beta-thymol. C1 [Levent, G.; Harvey, R. B.; Beier, R. C.; Genovese, K. J.; He, H. L.; Anderson, R. C.; Nisbet, D. J.] ARS, USDA, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. [Levent, G.; Ciftcioglu, G.] Istanbul Univ, Dept Food Hyg & Technol, Fac Vet Med, TR-34320 Istanbul, Turkey. RP Harvey, RB (reprint author), ARS, USDA, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM roger.harvey@ars.usda.gov FU National Pork Board [14-077] FX This project was funded in part by National Pork Board Grant 14-077. We thank Jackie Kotzur for excellent technical assistance. NR 18 TC 0 Z9 0 U1 4 U2 7 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2016 VL 79 IS 2 BP 299 EP 303 DI 10.4315/0362-028X.JFP-15-360 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DD2TR UT WOS:000369775600015 PM 26818992 ER PT J AU Jepsen, SM Harmon, TC Meadows, MW Hunsaker, CT AF Jepsen, S. M. Harmon, T. C. Meadows, M. W. Hunsaker, C. T. TI Hydrogeologic influence on changes in snowmelt runoff with climate warming: Numerical experiments on a mid-elevation catchment in the Sierra Nevada, USA SO JOURNAL OF HYDROLOGY LA English DT Article DE streamflow; Runoff; Snow; Climate warming; Hydrogeology; Hydrologic property ID WESTERN UNITED-STATES; SOIL-WATER STORAGE; SENSITIVITY-ANALYSIS; STREAMFLOW RESPONSE; ALPINE VEGETATION; CHANGE SCENARIOS; RIVER-BASINS; CALIFORNIA; FOREST; MODELS AB The role of hydrogeology in mediating long-term changes in mountain streamflow, resulting from reduced snowfall in a potentially warmer climate, is currently not well understood. We explore this by simulating changes in stream discharge and evapotranspiration from a mid-elevation, 1-km(2) catchment in the southern Sierra Nevada of California (USA) in response to reduced snowfall under warmer conditions, for a plausible range in subsurface hydrologic properties. Simulations are performed using a numerical watershed model, the Penn State Integrated Hydrologic Model (PIHM), constrained by observations from a meteorological station, stream gauge, and eddy covariance tower. We predict that the fraction of precipitation occurring as snowfall would decrease from approximately 47% at current conditions to 25%, 12%, and 5% for air temperature changes of +2, +4, and +6 degrees C. For each of these warming scenarios, changes in mean annual discharge and evapotranspiration simulated by the different plausible soil models show large ranges relative to averages, with coefficients of variation ranging from -3 to 3 depending on warming scenario. With warming and reduced snowfall, substrates with greater storage capacity show less soil moisture limitation on evapotranspiration during the late spring and summer, resulting in greater reductions in annual stream discharge. These findings indicate that the hydrologic response of mountain catchments to atmospheric warming and reduced snowfall may substantially vary across elevations with differing soil and regolith properties, a relationship not typically accounted for in approaches relying on space-for-time substitution, An additional implication of our results is that model simulations of annual stream discharge in response to snowfall-to-rainfall transitions may be relatively uncertain for study areas where subsurface properties are not well constrained. (C) 2015 Elsevier B.V. All rights reserved. C1 [Jepsen, S. M.; Harmon, T. C.] Univ Calif Merced, Sch Engn, Sci & Engn Bldg 2,5200 N Lake Rd, Merced, CA 95343 USA. [Meadows, M. W.] Univ Calif Merced, Sierra Nevada Res Inst, Sci & Engn Bldg,5200 N Lake Rd, Merced, CA 95343 USA. [Hunsaker, C. T.] US Forest Serv, USDA, Pacific Southwest Res Stn, 2081 E Sierra Ave, Fresno, CA 93710 USA. [Meadows, M. W.] Pacific Gas & Elect Co, POB 425, Auberry, CA 93602 USA. RP Jepsen, SM (reprint author), Univ Calif Merced, Sch Engn, Sci & Engn Bldg 2,5200 N Lake Rd, Merced, CA 95343 USA. EM sjepsen@ucmerced.edu; tharmon@ucmerced.edu; momr@pge.com; chunsaker@fs.fed.us FU US National Science Foundation (NSF) [CBET-1204841]; Inter-American Institute for Global Change Research (IAI) (NSF Award) [CRN3038, GEO-1128040] FX We thank the anonymous reviewers for their helpful comments on the manuscript. Technical assistance was provided by Otto Alvarez, Jacob Flanagan, Michael Goulden, Jon Herman, James Kirchner, Phil Saksa, Yuning Shi, and Xuan Yu. Funding was provided by US National Science Foundation (NSF) Award # CBET-1204841 (Water Sustainability & Climate Program) and by the Inter-American Institute for Global Change Research (IAI) CRN3038 (under NSF Award # GEO-1128040). Data were provided by the Pacific Southwest Research Station, USDA Forest Service, which operates the Kings River Experimental Watersheds, and by the US NSF Southern Sierra Critical Zone Observatory. NR 57 TC 3 Z9 3 U1 11 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB PY 2016 VL 533 BP 332 EP 342 DI 10.1016/j.jhydrol.2015.12.010 PG 11 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DD7EJ UT WOS:000370086200027 ER PT J AU Brooks, ES Dobre, M Elliot, WJ Wu, JQ Boll, J AF Brooks, Erin S. Dobre, Mariana Elliot, William J. Wu, Joan Q. Boll, Jan TI Watershed-scale evaluation of the Water Erosion Prediction Project (WEPP) model in the Lake Tahoe basin SO JOURNAL OF HYDROLOGY LA English DT Article DE WEPP; Soil erosion; Lake Tahoe basin; Sediment; Ungauged basins ID CALIFORNIA-NEVADA; SEDIMENT YIELD; HYDROLOGY; RUNOFF; SIMULATION; USA; CATCHMENTS; INTERFACES; NUTRIENT AB Forest managers need methods to evaluate the impacts of management at the watershed scale. The Water Erosion Prediction Project (WEPP) has the ability to model disturbed forested hillslopes, but has difficulty addressing some of the critical processes that are important at a watershed scale, including baseflow and water yield. In order to apply WEPP to forested watersheds, we developed and assessed new approaches for simulating streamflow and sediment transport from large watersheds using WEPP. We created specific algorithms to spatially distribute soil, climate, and management input files for all the subwatersheds within the basin. The model enhancements were tested on five geologically and climatically diverse watersheds in the Lake Tahoe basin, USA. The model was run with minimal calibration to assess WEPP's ability as a physically-based model to predict streamflow and sediment delivery. The performance of the model was examined against 17 years of observed snow water equivalent depth, stream flow, and sediment load data. Only region-wide baseflow recession parameters related to the geology of the basin were calibrated with observed streamflow data. Close agreement between simulated and observed snow water equivalent, streamflow, and the distribution of fine (<20 mu m) and coarse (>20 mu m) sediments was achieved at each of the major watersheds located in the high-precipitation regions of the basin. Sediment load was adequately simulated in the drier watersheds; however, annual streamflow was overestimated. With the exception of the drier eastern region, the model demonstrated no loss in accuracy when applied without calibration to multiple watersheds across Lake Tahoe basin demonstrating the utility of the model as a management tool in gauged and ungauged basins. (C) 2015 Elsevier B.V. All rights reserved. C1 [Brooks, Erin S.; Dobre, Mariana] Univ Idaho, Biol & Agr Engn, POB 442060, Moscow, ID 83844 USA. [Elliot, William J.] USDA, FS Rocky Mt Res Stn, 1221 South Main, Moscow, ID 83843 USA. [Wu, Joan Q.] Washington State Univ, Puyallup Res & Extens Ctr, Biol Syst Engn, 2606 W Pioneer, Puyallup, WA 98371 USA. [Boll, Jan] Washington State Univ, Civil & Environm Engn, 405 Spokane St,Sloan 101,POB 642910, Pullman, WA 99164 USA. RP Dobre, M (reprint author), Univ Idaho, Biol & Agr Engn, POB 442060, Moscow, ID 83844 USA. EM ebrooks@uidaho.edu; mdobre@uidaho.edu; welliot@fs.fed.us; jwu@wsu.edu; j.boll@wsu.edu OI Dobre, Mariana/0000-0002-5593-4906 FU Southern Nevada Public Land Management Act FX This study was funded by Southern Nevada Public Land Management Act, Round 7. NR 57 TC 3 Z9 3 U1 10 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD FEB PY 2016 VL 533 BP 389 EP 402 DI 10.1016/j.jhydrol.2015.12.004 PG 14 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DD7EJ UT WOS:000370086200032 ER PT J AU Corby-Harris, V Meador, CAD Snyder, LA Schwan, MR Maes, P Jones, BM Walton, A Anderson, KE AF Corby-Harris, Vanessa Meador, Charlotte A. D. Snyder, Lucy A. Schwan, Melissa R. Maes, Patrick Jones, Beryl M. Walton, Alexander Anderson, Kirk E. TI Transcriptional, translational, and physiological signatures of undernourished honey bees (Apis mellifera) suggest a role for hormonal factors in hypopharyngeal gland degradation SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Apis mellifera; Nutrition; Hypopharyngeal gland ID PROGRAMMED CELL-DEATH; DIVISION-OF-LABOR; DROSOPHILA-MELANOGASTER; SALIVARY-GLANDS; CALLIPHORA-VOMITORIA; EXPRESSION ANALYSIS; AMBLYOMMA-HEBRAEUM; ECDYSTEROID TITER; IXODID TICK; L. COLONIES AB Honey bee colonies function as a superorganism, where facultatively sterile female workers perform various tasks that support the hive. Nurse workers undergo numerous anatomical and physiological changes in preparation for brood rearing, including the growth of hypopharyngeal glands (HGs). These glands produce the major protein fraction of a protein-and lipid-rich jelly used to sustain developing larvae. Pollen intake is positively correlated with HG growth, but growth in the first three days is similar regardless of diet, suggesting that initial growth is a pre-determined process while later HG development depends on nutrient availability during a critical window in early adulthood (>3 d). It is unclear whether the resultant size differences in nurse HG are simply due to growth arrest or active degradation of the tissue. To determine what processes cause such differences in HG size, we catalogued the differential expression of both gene transcripts and proteins in the HGs of 8 d old bees that were fed diets containing pollen or no pollen. 3438 genes and 367 proteins were differentially regulated due to nutrition. Of the genes and proteins differentially expressed, undernourished bees exhibited more gene and protein up-regulation compared to well-nourished bees, with the affected processes including salivary gland apoptosis, oogenesis, and hormone signaling. Protein secretion was virtually the only process up-regulated in well-nourished bees. Further assays demonstrated that inhibition of ultraspiracle, one component of the ecdysteroid receptor, in the fat body caused larger HGs. Undernourished bees also had higher acid phosphatase activity, a physiological marker of cell death, compared to well-nourished bees. These results support a connection between poor nutrition, hormonal signaling, and HG degradation. Published by Elsevier Ltd. C1 [Corby-Harris, Vanessa; Meador, Charlotte A. D.; Snyder, Lucy A.; Schwan, Melissa R.; Maes, Patrick; Jones, Beryl M.; Walton, Alexander; Anderson, Kirk E.] USDA, ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Corby-Harris, Vanessa; Anderson, Kirk E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Maes, Patrick] Univ Arizona, Grad Interdisciplinary Program Entomol & Insect S, Tucson, AZ 85719 USA. [Jones, Beryl M.] Univ Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA. [Walton, Alexander] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. RP Corby-Harris, V (reprint author), USDA, ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. EM vanessa.corby@ars.usda.gov FU NIEHS [ES06694]; NIH/NCI [CA023074]; NIH/NCRR [1S10 RR028868-01]; University of Arizona BIO5 Institute; United States Department of Agriculture FX The authors acknowledge the staff of the Carl Hayden Bee Research Center, the University of Arizona Genetics Core (http://uagc.arl.arizona.edu/), and the Arizona Proteomics Consortium (supported by NIEHS grant ES06694, NIH/NCI grant CA023074, NIH/NCRR grant 1S10 RR028868-01, and the University of Arizona BIO5 Institute). The project benefitted from discussions with Drs. Juergen Gadau, Alan Rawls, and Mark Carroll and from comments by Miguel Corona and Colin Brent on a previous version of the manuscript. This work was supported with internal funds from the United States Department of Agriculture. The ARS/USDA is an equal opportunity employer and provider. NR 72 TC 1 Z9 1 U1 1 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD FEB PY 2016 VL 85 BP 65 EP 75 DI 10.1016/j.jinsphys.2015.11.016 PG 11 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA DD7MS UT WOS:000370109300008 PM 26658137 ER PT J AU Sweitzer, RA Thompson, CM Green, RE Barrett, RH Purcell, KL AF Sweitzer, Richard A. Thompson, Craig M. Green, Rebecca E. Barrett, Reginald H. Purcell, Kathryn L. TI Survival of fishers in the southern Sierra Nevada region of California SO JOURNAL OF MAMMALOGY LA English DT Article DE California; fisher; MARK; Pekania pennanti; Sierra Nevada; survival ID MARTES-PENNANTI; AMERICAN MARTENS; ROCKY-MOUNTAINS; DISPERSAL; RODENTICIDES; REPRODUCTION; POPULATION; LANDSCAPE; HABITAT; MORTALITY AB Fishers in the western United States were recently proposed for listing under the U.S. Endangered Species Act because of concerns for loss of suitable habitat and evidence of a diversity of mortality risks that reduce survival. One of 2 remnant populations of fishers in California is in the southern Sierra Nevada region, where we studied them at 2 research sites in the Sierra National Forest. Our objectives were to evaluate whether survival was lower for male fishers and dispersal-aged individuals or if survival varied seasonally. We captured and monitored 232 radiocollared fishers from March 2007 to March 2014 and used model analyses to identify important predictors of survival. Fifty-two percent (n = 120) of the radiocollared fishers died, and survival varied by sex and season, but not by age or between study sites. There was no evidence that dispersal-aged fishers experienced lower survival than older fishers. Annual survival trended lower for male (0.62 [95% CI 0.54-0.70]) compared to female fishers (0.72 [95% CI 0.67-0.78]), was lowest in the spring to mid-summer season (0.83 [95% CI 0.78-0.87]), and highest in late fall and winter (0.92 [95% CI 0.89-0.94]). Lower survival among male fishers appeared linked to males moving over large areas to locate mates, while lower survival for females was potentially related to high energetic cost of reproduction. It was possible but unknown if lower survival among all fishers in spring was linked to secondary exposure to toxicants dispersed around illicit marijuana grow sites. Six-month survival of juvenile fishers was 0.85 for females and 0.79 for males, but lower at 0.62 for females and 0.57 for males when adjusted for deaths before late September. Annual survival among adult female fishers was 20% lower than 0.90, a value that prior modeling suggested was required for population expansion in the overall southern Sierra Nevada. Survival data from our study imply a greater challenge for maintaining self-sustaining fisher populations in the southern Sierra Nevada region, and resource managers are working to mitigate several of the human-associated factors that limit population growth. C1 [Sweitzer, Richard A.] Great Basin Inst, 16750 Mt Rose Highway, Reno, NV 89511 USA. [Thompson, Craig M.; Green, Rebecca E.; Purcell, Kathryn L.] USDA, US Forest Serv, Pacific Southwest Res Stn, 2081 E Sierra Ave, Fresno, CA 93710 USA. [Barrett, Reginald H.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Sweitzer, RA (reprint author), Great Basin Inst, 16750 Mt Rose Highway, Reno, NV 89511 USA. EM sweitzerrick@gmail.com RI Purcell, Kathryn/S-2592-2016 FU U.S. Forest Service Region 5; U.S. Forest Service Pacific Southwest Research Station; U.S. Fish and Wildlife Service; California Department of Water Resources; California Department of Fish and Game; California Department of Forestry and Fire Protection; Sierra Nevada Conservancy; California Agricultural Experiment Station FX The field effort would not have been possible without help from a dedicated team of staff and volunteers including, J. Ashling, S. Bassing, A. Beaudette, J. Busiek, A. Cellar, T. Day, Z. Eads, T. Gorman, D. Hardeman, D. Jackson, W. Mitchell, B. Nieles, C. O'Brien, M. Ratchford, J. Ruthven, J. Schneiderman, W. Sicard, T. Thein, S. Vogel, R. Wise, T. Watson, and others on the northern site, and J. Garner, T. Brickly, J. Banaszak, C. Indelicato, N. Hebert, Z. Miller, B. Nichols, S. Rossler, T. Smith, Z. Stoll, and G. Watts on the southern site. We thank Forest Service pilots, J. Irving, S. Forkel, B. Bulfer, C. Haney, and especially J. Litton for aviation support. Local support at the northern site was facilitated by B. Persson, A. Otto, and A. Lombardo. We also thank 2 anonymous reviewers for comments and suggestions that helped improve the manuscript. This study was associated with the Sierra Nevada Adaptive Management Project (SNAMP) and is SNAMP publication no. 42. Our research was funded by U.S. Forest Service Region 5, U.S. Forest Service Pacific Southwest Research Station, U.S. Fish and Wildlife Service, California Department of Water Resources, California Department of Fish and Game, California Department of Forestry and Fire Protection, the Sierra Nevada Conservancy, and the California Agricultural Experiment Station. NR 80 TC 1 Z9 1 U1 12 U2 27 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2372 EI 1545-1542 J9 J MAMMAL JI J. Mammal. PD FEB PY 2016 VL 97 IS 1 BP 274 EP 286 DI 10.1093/jmammal/gyv177 PG 13 WC Zoology SC Zoology GA DC5AP UT WOS:000369232600026 ER PT J AU Tagtow, A Rahavi, E Bard, S Stoody, EE Casavale, K Mosher, A AF Tagtow, Angie Rahavi, Elizabeth Bard, Sasha Stoody, Eve Essery Casavale, Kellie Mosher, Amber TI Coming Together to Communicate the 2015-2020 Dietary Guidelines for Americans SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article C1 [Tagtow, Angie; Rahavi, Elizabeth; Bard, Sasha; Stoody, Eve Essery] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. [Casavale, Kellie; Mosher, Amber] US Dept HHS, Off Dis Prevent & Hlth Promot, Rockville, MD USA. RP Tagtow, A (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. NR 3 TC 2 Z9 2 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2016 VL 116 IS 2 BP 209 EP 212 DI 10.1016/j.jand.2015.12.010 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DD0KH UT WOS:000369608400004 PM 26822984 ER PT J AU Qian, SS Harmel, RD AF Qian, Song S. Harmel, R. Daren TI APPLYING STATISTICAL CAUSAL ANALYSES TO AGRICULTURAL CONSERVATION: ACASE STUDY EXAMINING P LOSS IMPACTS SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE causal inference; conservation practices; multilevel modeling; observational data; propensity score ID NUTRIENT LOAD; PHOSPHORUS; COMPILATION; WATER; RIVER AB Estimating the effect of agricultural conservation practices on reducing nutrient loss using observational data can be confounded by factors such as differing crop types and management practices. As we may not have the full knowledge of these confounding factors, conventional statistical meta-analysis methods can be misleading. We discuss the use of two statistical causal analysis methods for quantifying the effects of water and soil conservation practices in reducing P loss from agricultural fields. With the propensity score method, a subset of data was used to form a treatment group and a control group with similar distributions of confounding factors. With the multilevel modeling method, data were stratified based on important confounding factors, and the conservation practice effect was evaluated for each stratum. Both methods resulted in similar estimates of the conservation practice effect (total P load reduction avg. similar to 70%). In addition, both methods show evidence of conservation practices reducing the incremental increase in total P export per unit increase in fertilizer application. These results are presented as examples of the types of outcomes provided by statistical causal analyses, not to provide definitive estimates of P loss reduction. The enhanced meta-analysis methods presented within are applicable for improved assessment of agricultural practices and their effects and can be used for providing realistic parameter values for watershed-scale modeling. C1 [Qian, Song S.] Univ Toledo, Dept Environm Sci, 2801 West Bancroft St,MS604, Toledo, OH 43606 USA. [Harmel, R. Daren] ARS, USDA, Temple, TX 76502 USA. RP Qian, SS (reprint author), Univ Toledo, Dept Environm Sci, 2801 West Bancroft St,MS604, Toledo, OH 43606 USA. EM song.qian@utoledo.edu FU University of Toledo Research Council; 4R Nutrient Stewardship FX We thank Pete Richards, Ibrahim Alameddine, and Kenneth H. Reckhow for constructive comments and suggestions. SSQ was partially supported by grants from the University of Toledo Research Council and the 4R Nutrient Stewardship. NR 23 TC 0 Z9 0 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD FEB PY 2016 VL 52 IS 1 BP 198 EP 208 DI 10.1111/1752-1688.12377 PG 11 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DD5XV UT WOS:000369999100013 ER PT J AU White, M Gambone, M Yen, H Daggupati, P Bieger, K Deb, D Arnold, J AF White, Michael Gambone, Marilyn Yen, Haw Daggupati, Prasad Bieger, Katrin Deb, Debjani Arnold, Jeff TI DEVELOPMENT OF A CROPLAND MANAGEMENT DATASET TO SUPPORT US SWAT ASSESSMENTS SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE Soil and Water Assessment Tool; management; water quality; agriculture ID UNITED-STATES; NUTRIENT LOAD; DATABASE; CALIBRATION; ECOREGIONS; VALIDATION AB The Soil and Water Assessment Tool (SWAT) is widely used in the United States (U.S.) to simulate hydrology and water quality simulation. Process-based models like SWAT require a great deal of data to accurately represent the natural world, including topography, land use, soils, weather, and management. With the exception of management, all these data are available nationally from multiple sources. To date, credible SWAT studies in the U.S. have assembled suitable management data (operation scheduling, fertilization application rates, and plant growth parameterization). In this research, we develop a national management database for SWAT using existing U.S. Department of Agriculture data sources. These data are compatible with existing SWAT interfaces and are relatively easy to use. Although management data from local sources is preferred, these data are not always available. This work is intended to fill this void with more reasonable management data than the existing defaults. This national database covers all major cultivated crops and should facilitate improved SWAT applications in the U.S. These data were tested in two case studies and found to produce satisfactory SWAT predictions. The database developed in this research is freely available on the web. C1 [White, Michael; Gambone, Marilyn] ARS, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. [Yen, Haw; Bieger, Katrin] Texas A&M AgriLife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Daggupati, Prasad; Deb, Debjani] Texas A&M Univ, Spatial Sci Lab, College Stn, TX 77843 USA. RP White, M (reprint author), ARS, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. EM mike.white@ars.usda.gov RI Daggupati, Prasad/D-8886-2017 OI Daggupati, Prasad/0000-0002-7044-3435 FU U.S. Department of Agriculture, Agricultural Research Service FX This work was funded by the U.S. Department of Agriculture, Agricultural Research Service. NR 21 TC 0 Z9 0 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD FEB PY 2016 VL 52 IS 1 BP 269 EP 274 DI 10.1111/1752-1688.12384 PG 6 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DD5XV UT WOS:000369999100018 ER PT J AU Latif, QS Ellis, MM Amundson, CL AF Latif, Quresh S. Ellis, Martha M. Amundson, Courtney L. TI A broader definition of occupancy: Comment on Hayes and Monfils SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Letter ID DETECTION PROBABILITY; RESOURCE SELECTION; POINT COUNTS; MARSH BIRDS; MODELS; INFERENCE; POPULATIONS; ANIMALS; DENSITY; HABITAT C1 [Latif, Quresh S.; Ellis, Martha M.] US Forest Serv, Rocky Mt Res Stn, 1648 S Seventh Ave, Bozeman, MT 59717 USA. [Amundson, Courtney L.] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. RP Latif, QS (reprint author), US Forest Serv, Rocky Mt Res Stn, 1648 S Seventh Ave, Bozeman, MT 59717 USA. EM qlatif@fs.fed.us NR 32 TC 2 Z9 2 U1 3 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2016 VL 80 IS 2 BP 192 EP 194 DI 10.1002/jwmg.1022 PG 3 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DD3JE UT WOS:000369817500002 ER PT J AU Fremgen, AL Hansen, CP Rumble, MA Gamo, RS Millspaugh, JJ AF Fremgen, Aleshia L. Hansen, Christopher P. Rumble, Mark A. Gamo, R. Scott Millspaugh, Joshua J. TI Male greater sage-grouse detectability on leks SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE abundance; Centrocercus urophasianus; detectability; detection; greater sage-grouse; index; lek counts; sightability; Wyoming ID POINT-COUNT SURVEYS; SIGHTABILITY MODEL; DETECTION PROBABILITIES; VISUAL OBSTRUCTION; PRAIRIE-CHICKENS; AERIAL SURVEYS; POPULATION; ATTENDANCE; CALIFORNIA; ABUNDANCE AB It is unlikely all male sage-grouse are detected during lek counts, which could complicate the use of lek counts as an index to population abundance. Understanding factors that influence detection probabilities will allow managers to more accurately estimate the number of males present on leks. We fitted 410 males with global positioning system and very high frequency transmitters, and uniquely identifiable leg-bands over 4 years in Carbon County, Wyoming. We counted male sage-grouse using commonly used lek-count protocols and evaluated variables associated with our ability to detect marked males using sightability surveys on 22 leks. We evaluated detection probabilities of male sage-grouse based on factors related to bird characteristics such as age or posture, lek and group size, lek characteristics such as vegetation cover or aspect, light conditions, weather, and observer. We then applied the detection probabilities to more accurately estimate male counts on leks. Detection probabilities were generally high (x=0.87) but varied among leks from 0.77 to 0.93. Male sage-grouse detection declined with increasing sagebrush height and bare ground and increased with more snow cover. Detection probabilities were also lower when observers counted from a higher elevation than the lek. Our sightability models predicted detection well and can be used to accurately estimate male abundance on leks from lek counts, which is especially useful where accurate abundance estimates are required or inference about population status is based on only 1 count. Further, it is important to consider lek attendance as a component of counts on leks because it affects availability of male sage-grouse for detection during lek counts. Detection can be maximized by conducting lek counts from 30minutes before sunrise to 30minutes after sunrise, although current protocols recommend lek counts can be performed up to 1hour after sunrise. Detection can also be maximized by conducting lek counts 2 days after snowfall, which maximizes attendance and detection. (c) 2015 The Wildlife Society. C1 [Fremgen, Aleshia L.; Hansen, Christopher P.; Millspaugh, Joshua J.] Univ Missouri, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Rumble, Mark A.] US Forest Serv, Rocky Mt Res Stn, 8221 S Highway 16, Rapid City, SD 57702 USA. [Gamo, R. Scott] Wyoming Game & Fish, 5400 Bishop Blvd, Cheyenne, WY 82006 USA. RP Fremgen, AL (reprint author), Univ Missouri, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM FremgenAL@missouri.edu FU federal and state wildlife agencies; wind energy partners under oversight of the National Wind Coordinating Collaborative; University of Missouri; U.S. Department of Energy Office of Energy Efficiency and Renewable Energy; Wind and Water Power Technologies [DE-AC36-08GO28308]; National Renewable Energy Laboratory [XFT-1-11321-01]; National Fish and Wildlife Foundation; U.S. Forest Service Rocky Mountain Research Station [10-JV-11221632-215, 13-JV-11221632-046]; Western Association of Fish and Wildlife Agencies [11-CO-11221632-068, 12-CO-11221632-181, 13-CO-11221632-007] FX F. R. Thompson, III, H. S. He, N. J. Wojcik, and A. M. Widmer provided comments on earlier drafts of this manuscript. We thank the OTR, Power Company of Wyoming, LLC, J. M. Theesfeld, and other Carbon County landowners for land access. J. C. Brockman, A. N. Coleman, M. E. Doherty, C. A. Doyle, A. Foster, J. A. Fox, R. L. Golden, M. B. Gonnerman, A. M. Miller, M. J. Peterson, S. L. Robatcek, B. N. Towery, and M. P. Womack provided technical field assistance. M. J. Paul, N. J. Wojcik, J. W. Kehmeier, R. Hay, R. B. Duncan, and T. Grosch assisted with lek counts. Primos Hunting provided financial assistance for ground blinds. F. R. Thompson, III and T. W. Bonnot assisted with analyses. Funding was provided by federal and state wildlife agencies, and wind energy partners under the oversight of the National Wind Coordinating Collaborative. Funding sponsors include the University of Missouri, U.S. Department of Energy Office of Energy Efficiency and Renewable Energy, Wind and Water Power Technologies (DE-AC36-08GO28308), National Renewable Energy Laboratory (subcontract XFT-1-11321-01), National Fish and Wildlife Foundation, U.S. Forest Service Rocky Mountain Research Station (10-JV-11221632-215 and 13-JV-11221632-046), and Western Association of Fish and Wildlife Agencies (11-CO-11221632-068, 12-CO-11221632-181, and 13-CO-11221632-007). We thank C. L. Aldridge and D. D. Musil for providing helpful comments on an earlier draft that improved our manuscript. NR 64 TC 2 Z9 2 U1 1 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2016 VL 80 IS 2 BP 266 EP 274 DI 10.1002/jwmg.1001 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DD3JE UT WOS:000369817500010 ER PT J AU Morton, JM White, GC Hayward, GD Paetkau, D Bray, MP AF Morton, John M. White, Gary C. Hayward, Gregory D. Paetkau, David Bray, Martin P. TI Estimation of the brown bear population on the Kenai Peninsula, Alaska SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Alaska; brown bear; genetics; hair DNA; Kenai Peninsula; mark-recapture; population; Pradel model; Ursus arctos ID SOUTH-CENTRAL ALASKA; CAPTURE-RECAPTURE DATA; MARK-RECAPTURE; GRIZZLY BEARS; URSUS-ARCTOS; BRITISH-COLUMBIA; DNA; DENSITY; CANADA; HAIR AB The brown bear population on the Kenai Peninsula, Alaska, has not been empirically estimated previously because conventional aerial methods over this heavily forested landscape were infeasible. We applied a rapid field protocol to a DNA-based, mark-recapture approach on a large and tightly bounded sample frame to estimate brown bear abundance. We used lure to attract bears to barbed wire stations deployed in 145 9-kmx9-km cells systematically distributed across 10,200km(2) of available habitat on the Kenai National Wildlife Refuge and Chugach National Forest during 31 consecutive days in early summer 2010. Using 2 helicopters and 4 2-person field crews, we deployed the stations during a 6-day period and subsequently revisited these stations on 5 consecutive 5-day trap sessions. We extracted DNA to identify individual bears and developed capture histories as input to mark-recapture models. Combined with data from radio-telemetered bears, 243 brown bears were alive on the Kenai Peninsula in 2010, but we used only 99 females and 104 males in modeling. We used Akaike's Information Criterion selection and model averaging to estimate 428 (95% lognormal CI=353-539) brown bears (including all age classes) on the study area. Despite low recaptures rates, we achieved reasonable precision by ensuring geographic and demographic population closure through a spatially comprehensive sample frame and very short sampling window. We reduced bias by including information from rub trees and telemetered females (i.e., occasion 0). Extrapolating the density estimate of 42 bears/1,000 km(2) of available habitat on the study area to the Kenai Peninsula suggests a peninsula-wide population of 582 brown bears (95% lognormal CI=469-719). Despite a density estimate that is low compared to other coastal brown bear populations in Alaska and genetic evidence that suggests this peninsular population is insular, harvest management has been liberalized since 2012. We recommend this population estimate serve as the benchmark for future management. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Morton, John M.] US Fish & Wildlife Serv, Kenai Natl Wildlife Refuge, POB 2139, Soldotna, AK 99669 USA. [White, Gary C.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Hayward, Gregory D.] US Forest Serv, 161 East 1st Ave, Anchorage, AK 99501 USA. [Paetkau, David] Wildlife Genet Int, POB 274, Nelson, BC V1L 5P9, Canada. [Bray, Martin P.] US Forest Serv, Chugach Natl Forest, 161 East 1st Ave, Anchorage, AK 99501 USA. RP Morton, JM (reprint author), US Fish & Wildlife Serv, Kenai Natl Wildlife Refuge, POB 2139, Soldotna, AK 99669 USA. EM john_m_morton@fws.gov FU U.S. Fish and Wildlife Service; U.S. Forest Service FX We thank ADFG for sharing telemetry and genetics data. J. Boulanger, K. C. Kendall, T. L. McDonald, G. Pendleton, N. Roberts, J. A. Royle, K. Titus, L. J. Van Daele, and 2 anonymous ADFG personnel reviewed earlier versions of this report. M. I. Goldstein helped with the original proposal. Field personnel were M. L. Bowser, S. E. Bradley, T. D. Eskelin, J. Heydinger, N. J. Lester, A. Neurock, B. Parsons, S. D. Parkin, R. Ruffner, C. Whitaker and R. S. Zulueta. Technical support was provided by T. A. Burke, R. D. Ernst, E. A. Jozwiak, M. W. Laker, D. R. Magness and T. C. Mullet. D. M. Perez and D. J. Handley assisted with radio dispatching and flight monitoring. J. Benson led WGI lab efforts. A. J. Loranger and M. E. Novy provided managerial support. This study was funded by the U.S. Fish and Wildlife Service and U.S. Forest Service. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of any government agencies. NR 51 TC 0 Z9 0 U1 33 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2016 VL 80 IS 2 BP 332 EP 346 DI 10.1002/jwmg.1002 PG 15 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DD3JE UT WOS:000369817500016 ER PT J AU Chebrolu, KK Fritschi, FB Ye, SQ Krishnan, HB Smith, JR Gillman, JD AF Chebrolu, Kranthi K. Fritschi, Felix B. Ye, Songqing Krishnan, Hari B. Smith, James R. Gillman, Jason D. TI Impact of heat stress during seed development on soybean seed metabolome SO METABOLOMICS LA English DT Article DE Soybean; Seed development; Antioxidants; Germination; Abiotic stress ID MASS-SPECTROMETRY; GENETIC DIVERSITY; HIGH-TEMPERATURE; PROTEIN-CONTENT; ASCORBIC-ACID; ANTIOXIDANT; YIELD; IDENTIFICATION; CULTIVARS; PLANTS AB Seed development is a temperature-sensitive process that is much more vulnerable than vegetative tissues to abiotic stresses. High temperatures during soybean (Glycine max) seed development frequently results in seed with poor germination, increased incidence of pathogen infection, and decreased economic value. Climate change is expected to increase the incidence and severity of summer heatwaves, and the impact of heat stress on seed development will become more widespread during the course of the twenty-first century. Global metabolite profiles were contrasted between seed from heat-tolerant and heat-susceptible genotypes produced under 28/22 degrees C (control), 36/24 degrees C, and 42/26 degrees C day/night temperatures. Germination of seeds from the heat-susceptible genotype was strongly reduced (50 %) for the 36/24 degrees C treatment and completely inhibited for the 42/26 degrees C. In contrast, germination was unaffected for the heat-tolerant genotype for the 36/24 degrees C, and while strongly inhibited, some (25 %) seed from the heat-tolerant genotype were able to germinate even for the 42/26 degrees C treatment, and in general were less impacted by elevated temperatures as compared to a commonly grown high-yielding, but heat-sensitive genotype. A total of 275 seed metabolites were analyzed by three metabolite profiling methods, and genotype-specific differences and temperature specific differences were identified. A diverse set of antioxidant metabolites, including tocopherols, flavonoids, phenylpropanoids, and ascorbate precursors were found to be enriched in seed of the heat tolerant genotype. The generally greater abundance of these metabolites at both temperatures in the tolerant genotype indicates that these compounds are likely responsible, at least in part, for the greater tolerance to high temperatures during seed development. C1 [Chebrolu, Kranthi K.; Fritschi, Felix B.; Ye, Songqing] Univ Missouri, Div Plant Sci, Columbia, MO USA. [Smith, James R.] USDA ARS, Stoneville, MS 38776 USA. [Krishnan, Hari B.; Gillman, Jason D.] Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. RP Gillman, JD (reprint author), Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. EM jason.gillman@ars.usda.gov FU United Soybean Board [1420-532-5613]; USDA-Agricultural Research Service FX The authors would like to acknowledge the superb technical assistance of USDA-ARS technicians Alexandria Berghaus, and Jeremy Mullis. This work was partially supported by two grants to F. B. Fritschi, J.D. Gillman and J.R. Smith from United Soybean Board (Project No. 1420-532-5613), and by project funds from the USDA-Agricultural Research Service. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. The US Department of Agriculture, Agricultural Research Service, Midwest Area, is an equal opportunity, affirmative action employer and all agency services are available without discrimination. NR 55 TC 2 Z9 2 U1 13 U2 39 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD FEB PY 2016 VL 12 IS 2 AR 28 DI 10.1007/s11306-015-0941-1 PG 14 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DC6QE UT WOS:000369343900018 ER PT J AU Ma, YY Smith, CE Lai, CQ Irvin, MR Parnell, LD Lee, YC Pham, LD Aslibekyan, S Claas, SA Tsai, MY Borecki, IB Kabagambe, EK Ordovas, JM Absher, DM Arnett, DK AF Ma, Yiyi Smith, Caren E. Lai, Chao-Qiang Irvin, Marguerite R. Parnell, Laurence D. Lee, Yu-Chi Pham, Lucia D. Aslibekyan, Stella Claas, Steven A. Tsai, Michael Y. Borecki, Ingrid B. Kabagambe, Edmond K. Ordovas, Jose M. Absher, Devin M. Arnett, Donna K. TI The effects of omega-3 polyunsaturated fatty acids and genetic variants on methylation levels of the interleukin-6 gene promoter SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE DNA methylation; Gene-by-environment interaction; Genetic variant; Interleukin-6; n-3 polyunsaturated fatty acids ID LIPID-LOWERING DRUGS; DNA METHYLATION; INFLAMMATORY BIOMARKERS; DIET NETWORK; ERYTHROCYTE-MEMBRANES; SYSTEMIC INFLAMMATION; METABOLIC SYNDROME; MONONUCLEAR-CELLS; HEALTHY-MEN; MARKERS AB ScopeOmega-3 PUFAs (n-3 PUFAs) reduce IL-6 gene expression, but their effects on transcription regulatory mechanisms are unknown. We aimed to conduct an integrated analysis with both population and in vitro studies to systematically explore the relationships among n-3 PUFA, DNA methylation, single nucleotide polymorphisms (SNPs), gene expression, and protein concentration of IL6. Methods and resultsUsing data in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study and the Encyclopedia of DNA Elements (ENCODE) consortium, we found that higher methylation of IL6 promoter cg01770232 was associated with higher IL-6 plasma concentration (p = 0.03) and greater IL6 gene expression (p = 0.0005). Higher circulating total n-3 PUFA was associated with lower cg01770232 methylation (p = 0.007) and lower IL-6 concentration (p = 0.02). Moreover, an allele of IL6 rs2961298 was associated with higher cg01770232 methylation (p = 2.55x10(-7)). The association between n-3 PUFA and cg01770232 methylation was dependent on rs2961298 genotype (p = 0.02), but higher total n-3 PUFA was associated with lower cg01770232 methylation in the heterozygotes (p = 0.04) not in the homozygotes. ConclusionHigher n-3 PUFA is associated with lower methylation at IL6 promoter, which may be modified by IL6 SNPs. C1 [Ma, Yiyi; Smith, Caren E.; Lai, Chao-Qiang; Parnell, Laurence D.; Lee, Yu-Chi; Pham, Lucia D.; Ordovas, Jose M.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ma, Yiyi] Boston Univ, Dept Med, Biomed Genet, Boston, MA 02215 USA. [Irvin, Marguerite R.; Aslibekyan, Stella; Claas, Steven A.; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Kabagambe, Edmond K.] Vanderbilt Univ, Dept Med, 221 Kirkland Hall, Nashville, TN 37235 USA. [Ordovas, Jose M.] CNIC, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. [Absher, Devin M.] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. RP Ma, YY (reprint author), Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA.; Ma, YY (reprint author), Boston Univ, Dept Med, Biomed Genet, Boston, MA 02215 USA. EM yiyima@bu.edu OI Claas, Steven/0000-0001-9789-8395 FU National Heart Lung and Blood Institute [U01HL072524-04, 5R01HL1043135-04]; National Institute of Neurological Disorders and Stroke [T32NS054584]; U.S. Department of Agriculture [58-1950-0-014]; [K08 HL112845] FX This study is funded by National Heart Lung and Blood Institute Grant U01HL072524-04 and 5R01HL1043135-04, and the National Institute of Neurological Disorders and Stroke Grant T32NS054584. C. E. S. is supported by K08 HL112845. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. All authors read and approved the final manuscript. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 41 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD FEB PY 2016 VL 60 IS 2 BP 410 EP 419 DI 10.1002/mnfr.201500436 PG 10 WC Food Science & Technology SC Food Science & Technology GA DD5KO UT WOS:000369962800016 PM 26518637 ER PT J AU Gomez-Polo, P Alomar, O Castane, C Aznar-Fernandez, T Lundgren, JG Pinol, J Agusti, N AF Gomez-Polo, Priscila Alomar, Oscar Castane, Cristina Aznar-Fernandez, Thais Lundgren, Jonathan G. Pinol, Josep Agusti, Nuria TI Understanding trophic interactions of Orius spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods SO PEST MANAGEMENT SCIENCE LA English DT Article DE conventional PCR; gut-content analysis; high-throughput sequencing; Orius; real-time PCR; trophic relationships ID REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; GUT-CONTENT ANALYSIS; BIOLOGICAL-CONTROL; GENERALIST PREDATOR; CONVENTIONAL PCR; HETEROPTERA ANTHOCORIDAE; INTRAGUILD PREDATION; PREY PREFERENCE; VEGETABLE CROPS AB BACKGROUND: The aphid Nasonovia ribisnigri (Mosley) (Hemiptera: Aphididae) and the thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) are common pests in Mediterranean lettuce crops, where Orius spp. are common generalist predators. Predation by Orius spp. was studied in a lettuce plot by conventional polymerase chain reaction (PCR) and real-time PCR analyses using specific primers of both main pests. Also, high-throughput sequencing was used to have a wider approach of the diet of these predators in natural field conditions. RESULTS: Molecular analyses indicated a higher predation on N. ribisnigri in spring and on F. occidentalis in summer. Predation on alternative prey, like Collembola, was also found in both seasons. Real-time PCR was more sensitive than conventional PCR in showing the target trophic links, whereas high-throughput sequencing revealed predation on other natural enemies - intraguild predation (IGP), showing other trophic interactions of Orius majusculus within the studied ecosystem. CONCLUSIONS: This study gives important information about the trophic relationships present in Mediterranean lettuce crops in different periods of the year. The detected predation by Orius spp. on alternative prey, as well as on other natural enemies, should be further investigated to clarify whether it adds or detracts to the biological control of N. ribisnigri and F. occidentalis. (C) 2015 Society of Chemical Industry C1 [Gomez-Polo, Priscila; Alomar, Oscar; Castane, Cristina; Aznar-Fernandez, Thais; Agusti, Nuria] IRTA, Ctra Cabrils,Km 2, E-08348 Barcelona, Spain. [Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD USA. [Pinol, Josep] Univ Autonoma Barcelona, E-08193 Barcelona, Spain. [Pinol, Josep] CREAF, Barcelona, Spain. RP Agusti, N (reprint author), IRTA, Ctra Cabrils,Km 2, E-08348 Barcelona, Spain. EM nuria.agusti@irta.cat RI Alomar, Oscar/A-1520-2009; Agusti, Nuria/C-8318-2011 OI Alomar, Oscar/0000-0002-8756-871X; Agusti, Nuria/0000-0002-5908-4873 FU Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2008-00546, AGL2011-24349]; FPI grant - MINECO FX We are very grateful to Dr JJ Lopez-Moya (CRAG) and A Fereres (ICA-CSIC) for providing specimens of N. ribisnigri, and to V Vega (IRTA), J Fergen (USDA-ARS) and G Mir (CRAG) for technical support. This work has been funded by the Spanish Ministry of Economy and Competitiveness (MINECO) (Projects AGL2008-00546 and AGL2011-24349). P Gomez-Polo was supported by an FPI grant funded by MINECO. NR 58 TC 2 Z9 3 U1 8 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2016 VL 72 IS 2 BP 272 EP 279 DI 10.1002/ps.3989 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA DD2AA UT WOS:000369723500007 PM 25641943 ER PT J AU Gao, SD Sosnoskie, LM Cabrera, JA Qin, RJ Hanson, BD Gerik, JS Wang, D Browne, GT Thomas, JE AF Gao, Suduan Sosnoskie, Lynn M. Cabrera, Jose Alfonso Qin, Ruijun Hanson, Bradley D. Gerik, James S. Wang, Dong Browne, Greg T. Thomas, John E. TI Fumigation efficacy and emission reduction using low-permeability film in orchard soil fumigation SO PEST MANAGEMENT SCIENCE LA English DT Article DE 1,3-dichloropropene; chloropicrin; fumigant application; methyl bromide; nematode; Phytophthora; shank injection; soil fumigation; weed ID PERENNIAL CROP NURSERIES; METHYL-BROMIDE; CHLOROPICRIN EMISSIONS; VAPOR SAFE; 1,3-DICHLOROPROPENE; TIF; ALTERNATIVES; DENSITIES; FUMIGANTS; MEDIA AB BACKGROUND: Many orchards use fumigation to control soilborne pests prior to replanting. Controlling emissions ismandatory to reduce air pollution in California. This research evaluated the effects of plastic film type [polyethylene (PE) or totally impermeable film (TIF)], application rate of Telone C35 [full (610 kg ha(-1)), 2/3 or 1/3 rates] and carbonation at 207 kPa on fumigant transport (emission and in soil) and efficacy. RESULTS: While increasing fumigant concentrations under the tarp, TIF reduced emissions >95%(similar to 2% and <1% of total applied 1,3-dichloropropene and chloropicrin respectively) relative to bare soil, compared with similar to 30% reduction by PE. All fumigation treatments, regardless of film type, provided good nematode control above 100 cm soil depth; however, nematode survival was high at deeper depths. Weed emergence was mostly affected by tarping and fumigant rate, with no effects from the carbonation. CONCLUSION: TIF can effectively reduce fumigant emissions. Carbonation under the studied conditions did not improve fumigant dispersion and pest control. The 2/3 rate with TIF controlled nematodes as effectively as the full rate in bare soil or under the PE film to 100 cm soil depth. However, control of nematodes in deeper soil remains a challenge for perennial crops. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Gao, Suduan; Gerik, James S.; Wang, Dong] USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Sosnoskie, Lynn M.; Qin, Ruijun; Hanson, Bradley D.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Cabrera, Jose Alfonso] Bayer CropSci, Fresno, CA USA. [Browne, Greg T.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA USA. [Thomas, John E.] Univ Florida, Dept Soil andWater Sci, Gainesville, FL USA. RP Gao, SD (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM Suduan.Gao@ars.usda.gov FU Almond Board of California; USDA-ARS Pacific Area-Wide Pest Management Program for Integrated Methyl Bromide Alternatives; NIFA Methyl Bromide Transition Grants Program [0222549] FX Funding support for this research was provided by the Almond Board of California, the USDA-ARS Pacific Area-Wide Pest Management Program for Integrated Methyl Bromide Alternatives and the NIFA Methyl Bromide Transition Grants Program (Grant No. 0222549). Fumigant, fumigation service and carbonation of fumigant were provided or performed by TriCal, Inc. (Hollister, CA, USA). Personnel supporting this research include Robert Shenk, Aileen Hendratna, Allison Marquez, Jim Gartung, Patricia Mungur, Matt Gonzales, Stella Zambrzuski, Alex Jimenez and Carl Hawk from the San Joaquin Valley Agricultural Sciences Center, USDA-ARS at Parlier, California. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 0 Z9 0 U1 4 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2016 VL 72 IS 2 BP 306 EP 314 DI 10.1002/ps.3993 PG 9 WC Agronomy; Entomology SC Agriculture; Entomology GA DD2AA UT WOS:000369723500011 PM 25684360 ER PT J AU Zhang, JC Huang, YB Yuan, L Yang, GJ Chen, LP Zhao, CJ AF Zhang, Jingcheng Huang, Yanbo Yuan, Lin Yang, Guijun Chen, Liping Zhao, Chunjiang TI Using satellite multispectral imagery for damage mapping of armyworm (Spodoptera frugiperda) in maize at a regional scale SO PEST MANAGEMENT SCIENCE LA English DT Article DE maize; armyworm; multispectral remote sensing; mapping; modified soil-adjusted vegetation index ID ADJUSTED VEGETATION INDEX; HYPERSPECTRAL MEASUREMENTS; REFLECTANCE MEASUREMENTS; PRECISION AGRICULTURE; WHEAT; DISEASES; LEAF; IDENTIFICATION; OPPORTUNITIES; INFESTATION AB BACKGROUND: Armyworm, a destructive insect for maize, has caused a wide range of damage in both China and the United States in recent years. To obtain the spatial distribution of the damage area, and to assess the damage severity, a fast and accurate loss assessment method is of great importance for effective administration. The objectives of this study were to determine suitable spectral features for armyworm detection and to develop a mapping method at a regional scale on the basis of satellite remote sensing image data. RESULTS: Armyworm infestation can cause a significant change in the plant's leaf area index, which serves as a basis for infestation monitoring. Among the number of vegetation indices that were examined for their sensitivity to insect damage, the modified soil-adjusted vegetation index was identified as the optimal vegetation index for detecting armyworm. A univariate model relying on two-date satellite images significantly outperformed a multivariate model, with the overall accuracy increased from 0.50 to 0.79. CONCLUSION: A mapping method for monitoring armyworm infestation at a regional scale has been developed, based on a univariate model and two-date multispectral satellite images. The successful application of this method in a typical armyworm outbreak event in Tangshan, Hebei Province, China, demonstrated the feasibility of the method and its promising potential for implementation in practice. (C) 2015 Society of Chemical Industry C1 [Zhang, Jingcheng; Yuan, Lin; Yang, Guijun; Chen, Liping; Zhao, Chunjiang] Beijing Res Ctr Informat Technol Agr, Beijing 100097, Peoples R China. [Zhang, Jingcheng; Yuan, Lin; Yang, Guijun; Chen, Liping; Zhao, Chunjiang] Natl Engn Res Ctr Informat Technol Agr, Beijing, Peoples R China. [Zhang, Jingcheng; Yuan, Lin; Yang, Guijun; Chen, Liping; Zhao, Chunjiang] Minist Agr, Key Lab Informat Technol Agr, Beijing, Peoples R China. [Zhang, Jingcheng; Yuan, Lin; Yang, Guijun; Chen, Liping; Zhao, Chunjiang] Beijing Engn Res Ctr Agr Internet Things, Beijing, Peoples R China. [Huang, Yanbo] USDA ARS, CPSRU, 141 Expt Stn Rd, Stoneville, MS 38776 USA. RP Zhao, CJ (reprint author), Beijing Res Ctr Informat Technol Agr, Beijing 100097, Peoples R China.; Zhao, CJ (reprint author), Natl Engn Res Ctr Informat Technol Agr, Beijing, Peoples R China.; Zhao, CJ (reprint author), Minist Agr, Key Lab Informat Technol Agr, Beijing, Peoples R China.; Zhao, CJ (reprint author), Beijing Engn Res Ctr Agr Internet Things, Beijing, Peoples R China. EM zhaochjrs@126.com FU National Natural Science Foundation of China [41301476, 41271412]; Beijing Natural Science Foundation [4132029]; Beijing Nova Programme, China [Z151100000315059] FX This work was financially supported by the National Natural Science Foundation of China (Project Nos 41301476 and 41271412), the Beijing Natural Science Foundation (Project No. 4132029), and Beijing Nova Programme (No. Z151100000315059), China. The authors are grateful to Mrs Shuhong Cai from Hebei Plant Protection Agency and to Mr Weiguo Li and Mrs Hong Chang from the Beijing Research Centre for Information Technology in Agriculture for their assistance in data collection. NR 47 TC 1 Z9 1 U1 9 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2016 VL 72 IS 2 BP 335 EP 348 DI 10.1002/ps.4003 PG 14 WC Agronomy; Entomology SC Agriculture; Entomology GA DD2AA UT WOS:000369723500017 PM 25761201 ER PT J AU Conkle, JL Cabrera, JA Thomas, JE Wang, D Gan, J AF Conkle, Jeremy L. Cabrera, Jose Alfonso Thomas, John E. Wang, Dong Gan, Jay TI Effects of CO2 dissolution on phase distribution and degradation of dimethyl disulfide in soils under grape production SO PEST MANAGEMENT SCIENCE LA English DT Article DE methyl bromide alternatives; adsorption; Henry's law constant; half-life; carbonation ID SUPERCRITICAL CARBON-DIOXIDE; METHYL-BROMIDE; OFF-FLAVORS; FUMIGANTS; NEMATODES; BEHAVIOR AB BACKGROUND: Dimethyl disulfide (DMDS) is a fumigant recently registered in parts of the United States. The fumigant has high pesticidal activity, but does not disperse in soils as well as other fumigants. This study assessed the use of CO2 as a propellant to improve soil dispersion and diffusion by evaluating the partitioning and degradation of DMDS after carbonation in four vineyard soils collected in California. RESULTS: The soil with the highest organic carbon content (Clarksburg) had the highest soil-water partition coefficient (K-d) (P < 0.001), which increased after carbonation. However, DMDS sorption decreased in the Mecca and Fowler soils. Henry's law constant (K-h), which measures a compound's potential for partitioning between air and water, doubled from 0.04 to 0.10 with the addition of CO2, indicating less DMDS solubility. Carbonation did not negatively affect DMDS's half-lives in the different soils. CONCLUSION: While trials are needed for validation of field-scale impacts, carbonation had mixed effects on soil partitioning and no discernable impact on degradation, but greatly decreased DMDS water solubility. This indicates that carbonation could improve some facets of DMDS diffusion and dispersion, depending on soil properties (carbon content and moisture), without greatly affecting its other behaviors. (C) 2015 Society of Chemical Industry C1 [Conkle, Jeremy L.; Cabrera, Jose Alfonso; Gan, Jay] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Conkle, Jeremy L.] Texas A&M Univ, Dept Phys & Environm Sci, Corpus Christi, TX 78412 USA. [Cabrera, Jose Alfonso; Wang, Dong] USDA ARS, Water Management Res Unit, Parlier, CA USA. [Cabrera, Jose Alfonso] Bayer CropSci, Fresno, CA USA. [Thomas, John E.] Univ Florida, Dept Soil & Water Sci, Gainesville, FL USA. RP Conkle, JL (reprint author), Texas A&M Univ, Dept Phys & Environm Sci, Coastal Hlth & Water Qual Lab, Corpus Christi, TX 78412 USA.; Conkle, JL (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA.; Conkle, JL (reprint author), Texas A&M Univ, Dept Phys & Environm Sci, Corpus Christi, TX 78412 USA. EM jeremy.conkle@tamucc.edu RI Conkle, Jeremy/F-2772-2011 OI Conkle, Jeremy/0000-0003-2121-9231 FU USDA National Institute of Food and Agriculture through its Methyl Bromide Alternatives Transition Program [2011-51102-31185] FX Part of this study was funded with a grant award (2011-51102-31185) provided by the USDA National Institute of Food and Agriculture through its Methyl Bromide Alternatives Transition Program. NR 21 TC 0 Z9 0 U1 8 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2016 VL 72 IS 2 BP 349 EP 353 DI 10.1002/ps.4004 PG 5 WC Agronomy; Entomology SC Agriculture; Entomology GA DD2AA UT WOS:000369723500015 PM 25765585 ER PT J AU Earles, JM Sperling, O Silva, LCR McElrone, AJ Brodersen, CR North, MP Zwieniecki, MA AF Earles, J. Mason Sperling, Or Silva, Lucas C. R. McElrone, Andrew J. Brodersen, Craig R. North, Malcolm P. Zwieniecki, Maciej A. TI Bark water uptake promotes localized hydraulic recovery in coastal redwood crown SO PLANT CELL AND ENVIRONMENT LA English DT Article DE embolism; fog; foliar uptake; phellem; sequoia sempervirens ID FOLIAR ABSORPTION; XYLEM; EMBOLISM; TREES; FOG; PERMEABILITY; CAVITATION; DROUGHT; CONTEXT; FOREST AB Coastal redwood (Sequoia sempervirens), the world's tallest tree species, rehydrates leaves via foliar water uptake during fog/rain events. Here we examine if bark also permits water uptake in redwood branches, exploring potential flow mechanisms and biological significance. Using isotopic labelling and microCT imaging, we observed that water entered the xylem via bark and reduced tracheid embolization. Moreover, prolonged bark wetting (16 h) partially restored xylem hydraulic conductivity in isolated branch segments and whole branches. Partial hydraulic recovery coincided with an increase in branch water potential from about -5.5 +/- 0.4 to -4.2 +/- 0.3MPa, suggesting localized recovery and possibly hydraulic isolation. As bark water uptake rate correlated with xylem osmotic potential (R-2 = 0.88), we suspect a symplastic role in transferring water from bark to xylem. Using historical weather data from typical redwood habitat, we estimated that bark and leaves are wet more than 1000 h per year on average, with over 30 events being sufficiently long (>24 h) to allow for bark-assisted hydraulic recovery. The capacity to uptake biologically meaningful volumes of water via bark and leaves for localized hydraulic recovery throughout the crown during rain/fog events might be physiologically advantageous, allowing for relatively constant transpiration. C1 [Earles, J. Mason; Sperling, Or; North, Malcolm P.; Zwieniecki, Maciej A.] Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. [Earles, J. Mason] Univ Calif Davis, Grad Grp Ecol, One Shields Ave, Davis, CA 95616 USA. [Silva, Lucas C. R.] Dept Land Air & Water Resources, One Shields Ave, Davis, CA 95616 USA. [McElrone, Andrew J.] USDA ARS, Davis, CA 95616 USA. [McElrone, Andrew J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Brodersen, Craig R.] Yale Univ, Sch Forestry & Environm Studies, 195 Prospect St, New Haven, CT 06511 USA. [North, Malcolm P.] USDA Forest Serv, PSW Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. RP Earles, JM (reprint author), Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA.; Earles, JM (reprint author), Univ Calif Davis, Grad Grp Ecol, One Shields Ave, Davis, CA 95616 USA. EM jmearles@ucdavis.edu RI Silva, Lucas/E-1202-2016; Brodersen, Craig/J-1112-2016 OI Silva, Lucas/0000-0002-4838-327X; Brodersen, Craig/0000-0002-0924-2570 FU US Environmental Protection Agency STAR fellowship program FX J. M. E. received funding through the US Environmental Protection Agency STAR fellowship program. We would like to thank Gabrielle Bohlman, Michael Koontz, Jan Ng, Brian Smithers and Jens Stevens for helpful comments during manuscript preparation. Jessica Orozco provided invaluable assistance in setting up the fog chamber experiment and processing samples. NR 30 TC 4 Z9 4 U1 10 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD FEB PY 2016 VL 39 IS 2 BP 320 EP 328 DI 10.1111/pce.12612 PG 9 WC Plant Sciences SC Plant Sciences GA DD7XC UT WOS:000370137300008 ER PT J AU Li, PH Zhang, GY Gonzales, N Guo, YQ Hu, HH Park, S Zhao, J AF Li, Penghui Zhang, Gaoyang Gonzales, Naomi Guo, Yingqing Hu, Honghong Park, Sunghun Zhao, Jian TI Ca2+-regulated and diurnal rhythm-regulated Na+/Ca2+ exchanger AtNCL affects flowering time and auxin signalling in Arabidopsis SO PLANT CELL AND ENVIRONMENT LA English DT Article DE auxin response; flowering time; Na+/Ca2+ homeostasis; vacuole ID CIRCADIAN CLOCK; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; VACUOLAR MEMBRANE; CA2+ PUMPS; CALCIUM; THALIANA; GENES; HOMEOSTASIS; TRANSPORT AB Calcium (Ca2+) is vital for plant growth, development, hormone response and adaptation to environmental stresses, yet the mechanisms regulating plant cytosolic Ca2+ homeostasis are not fully understood. Here, we characterize an Arabidopsis Ca2+-regulated Na+/Ca2+ exchanger AtNCL that regulates Ca2+ and multiple physiological processes. AtNCL was localized to the tonoplast in yeast and plant cells. AtNCL appeared to mediate sodium (Na+) vacuolar sequestration and meanwhile Ca2+ release. The EF-hand domains within AtNCL regulated Ca2+ binding and transport of Ca2+ and Na+. Plants with diminished AtNCL expression were more tolerant to high CaCl2 but more sensitive to both NaCl and auxin; heightened expression of AtNCL rendered plants more sensitive to CaCl2 but tolerant to NaCl. AtNCL expression appeared to be regulated by the diurnal rhythm and suppressed by auxin. DR5::GUS expression and root responses to auxin were altered in AtNCL mutants. The auxin-induced suppression of AtNCL was attenuated in SLR/IAA14 and ARF6/8 mutants. The mutants with altered AtNCL expression also altered flowering time and FT and CO expression; FT may mediate AtNCL-regulated flowering time change. Therefore, AtNCL is a vacuolar Ca2+-regulated Na+/Ca2+ exchanger that regulates auxin responses and flowering time. C1 [Li, Penghui; Zhang, Gaoyang; Guo, Yingqing; Zhao, Jian] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430075, Peoples R China. [Gonzales, Naomi; Guo, Yingqing; Zhao, Jian] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. [Hu, Honghong] Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430075, Peoples R China. [Park, Sunghun] Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. RP Zhao, J (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430075, Peoples R China. EM jianzhao@mail.hzau.edu.cn RI Zhao, Jian/M-4237-2014 OI Zhao, Jian/0000-0002-4416-7334 FU Major State Basic Research Development Program of China (973 Program) [2013CB127001]; Fundamental Research Funds for the Central Universities [2013PY065]; Huazhong Agricultural University Scientific & Technological Self-innovation Foundation [2012RC007] FX We thank Dr Kendal Hirschi for full support on this work initiated in CNRC and for critical reading. This work was supported by the Major State Basic Research Development Program of China (973 Program 2013CB127001), The Fundamental Research Funds for the Central Universities (2013PY065) and Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (2012RC007) to J.Z. NR 78 TC 4 Z9 4 U1 5 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD FEB PY 2016 VL 39 IS 2 BP 377 EP 392 DI 10.1111/pce.12620 PG 16 WC Plant Sciences SC Plant Sciences GA DD7XC UT WOS:000370137300013 PM 26296956 ER PT J AU Souza, FVD Kaya, E Vieira, LD de Souza, EH Amorim, VBD Skogerboe, D Matsumoto, T Alves, AAC Ledo, CAD Jenderek, MM AF Duarte Souza, Fernanda Vidigal Kaya, Ergun Vieira, Livia de Jesus de Souza, Everton Hilo de Oliveira Amorim, Vanusia Batista Skogerboe, Dianne Matsumoto, Tracie Cunha Alves, Alfredo Augusto da Silva Ledo, Carlos Alberto Jenderek, Maria M. TI Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Ananas comosus; Ex situ conservation; Histology; Cryo-injuries; Liquid nitrogen; PVS2 ID CURAUA ANANAS-ERECTIFOLIUS; LEAF FIBER VARIETIES; ENCAPSULATION-VITRIFICATION; GENETIC INTEGRITY; POTATO; L.; APICES; GERMPLASM; PROTOCOL; GROWTH AB Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus' genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 degrees C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5-1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue. C1 [Duarte Souza, Fernanda Vidigal; Vieira, Livia de Jesus; de Souza, Everton Hilo; de Oliveira Amorim, Vanusia Batista; Cunha Alves, Alfredo Augusto; da Silva Ledo, Carlos Alberto] Embrapa Cassava & Fruits, BR-44380000 Cruz Das Almas, Bahia, Brazil. [Duarte Souza, Fernanda Vidigal; Kaya, Ergun; Vieira, Livia de Jesus; Skogerboe, Dianne; Cunha Alves, Alfredo Augusto; Jenderek, Maria M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Kaya, Ergun] Mugla Sitki Kocman Univ, Mol Biol & Genet Dept, Fac Sci, TR-48000 Koteki, Mugla, Turkey. [Vieira, Livia de Jesus; de Souza, Everton Hilo; de Oliveira Amorim, Vanusia Batista] CAPES, Coordinat Improvement Higher Educ Personnel, BR-44380000 Cruz Das Almas, Bahia, Brazil. [Matsumoto, Tracie] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Souza, FVD (reprint author), Embrapa Cassava & Fruits, BR-44380000 Cruz Das Almas, Bahia, Brazil. EM fernanda.souza@embrapa.br FU Office to Improve University Personnel (CAPES) of the Brazilian Ministry of Education FX To the Office to Improve University Personnel (CAPES) of the Brazilian Ministry of Education for the doctoral scholarships granted to L. de Jesus Vieira, E. H. de Souza and V. Amorim. We would like to thank Monica L. Rossi, CENA-USP, for her contribution in the microscopy analysis (SEM) and NAP-MEPA, ESALQ-USP for the use of the microscopy facilities and PROCAD/CAPES (CENA/USP-UFRB). NR 55 TC 1 Z9 1 U1 4 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD FEB PY 2016 VL 124 IS 2 BP 351 EP 360 DI 10.1007/s11240-015-0899-8 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DD5FL UT WOS:000369948000011 ER PT J AU Scully, ED Gries, T Sarath, G Palmer, NA Baird, L Serapiglia, MJ Dien, BS Boateng, AA Ge, ZX Funnell-Harris, DL Twigg, P Clemente, TE Sattler, SE AF Scully, Erin D. Gries, Tammy Sarath, Gautam Palmer, Nathan A. Baird, Lisa Serapiglia, Michelle J. Dien, Bruce S. Boateng, Akwasi A. Ge, Zhengxiang Funnell-Harris, Deanna L. Twigg, Paul Clemente, Thomas E. Sattler, Scott E. TI Overexpression of SbMyb60 impacts phenylpropanoid biosynthesis and alters secondary cell wall composition in Sorghum bicolor SO PLANT JOURNAL LA English DT Article DE lignin; monocot; monolignol; C4 grass; bioenergy; transgenic ID PHENYLALANINE AMMONIA-LYASE; MYB TRANSCRIPTION FACTOR; LACTATING DAIRY-COWS; LIGNIN BIOSYNTHESIS; ARABIDOPSIS-THALIANA; BIOMASS PRODUCTION; FORAGE SORGHUM; FERULIC ACID; L. MOENCH; MAIZE AB The phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. The global regulators of phenylpropanoid metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall composition and the levels of several other aromatic compounds, including anthocyanins and flavonoids. While transcription factors correlated with downregulation of the phenylpropanoid biosynthesis pathway have been identified in several grass species, few transcription factors linked to activation of this pathway have been identified in C4 grasses, some of which are being developed as dedicated bioenergy feedstocks. In this study we investigated the role of SbMyb60 in lignin biosynthesis in sorghum (Sorghum bicolor), which is a drought-tolerant, high-yielding biomass crop. Ectopic expression of this transcription factor in sorghum was associated with higher expression levels of genes involved in monolignol biosynthesis, and led to higher abundances of syringyl lignin, significant compositional changes to the lignin polymer and increased lignin concentration in biomass. Moreover, transgenic plants constitutively overexpressing SbMyb60 also displayed ectopic lignification in leaf midribs and elevated concentrations of soluble phenolic compounds in biomass. Results indicate that overexpression of SbMyb60 is associated with activation of monolignol biosynthesis in sorghum. SbMyb60 represents a target for modification of plant cell wall composition, with the potential to improve biomass for renewable uses. C1 [Scully, Erin D.; Gries, Tammy; Sarath, Gautam; Palmer, Nathan A.; Funnell-Harris, Deanna L.; Sattler, Scott E.] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Scully, Erin D.; Sattler, Scott E.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Baird, Lisa] Univ San Diego, Shiley Ctr Sci & Technol, Dept Biol, San Diego, CA 92110 USA. [Serapiglia, Michelle J.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Dien, Bruce S.] USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Ge, Zhengxiang; Clemente, Thomas E.] Univ Nebraska, Dept Agron & Hort, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA. [Funnell-Harris, Deanna L.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Twigg, Paul] Univ Nebraska Kearney, Dept Biol, Kearney, NE 68849 USA. RP Sattler, SE (reprint author), Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA.; Sattler, SE (reprint author), Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. EM Scott.Sattler@ars.usda.gov OI Dien, Bruce/0000-0003-3863-6664 FU United States Department of Agriculture - National Institute of Food and Agriculture AFRI [2011-67009-30026]; USDA-ARS, CRIS [3042-21220-032-00D, 3042-21000-030-00D] FX We thank John Toy and Nathan Peroutka-Bigus for technical assistance and Pat O'Neill for statistical support. This research was supported by the United States Department of Agriculture - National Institute of Food and Agriculture AFRI grant number 2011-67009-30026 (SES, TEC, and DLF-H) and additional funding from USDA-ARS, CRIS projects 3042-21220-032-00D (SES DLF-H) and 3042-21000-030-00D (GS, NAP). The US Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. NR 70 TC 3 Z9 3 U1 13 U2 56 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD FEB PY 2016 VL 85 IS 3 BP 378 EP 395 DI 10.1111/tpj.13112 PG 18 WC Plant Sciences SC Plant Sciences GA DD3XT UT WOS:000369857400004 PM 26712107 ER PT J AU Harnly, J Chen, P Sun, JH Huang, HL Colson, KL Yuk, J McCoy, JAH Reynaud, DTH Harrington, PB Fletcher, EJ AF Harnly, James Chen, Pei Sun, Jianghao Huang, Huilian Colson, Kimberly L. Yuk, Jimmy McCoy, Joe-Ann H. Reynaud, Danica T. Harbaugh Harrington, Peter B. Fletcher, Edward J. TI Comparison of Flow Injection MS, NMR, and DNA Sequencing: Methods for Identification and Authentication of Black Cohosh (Actaea racemosa) SO PLANTA MEDICA LA English DT Article DE Actaea racemose; Ranunculaceae; NMR; FIMS; DNA sequencing ID PRINCIPAL COMPONENT ANALYSIS; BUILDING EXPERT-SYSTEMS; PRODUCTS; H-1-NMR; CLASSIFICATION; PROBABILITY; VALIDATION; MODELS; CURVE AB Flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry, two metabolic fingerprinting methods, and DNA sequencing were used to identify and authenticate Actaea species. Initially, samples of Actaea racemosa from a single source were distinguished from other Actaea species based on principal component analysis and soft independent modeling of class analogies of flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry metabolic fingerprints. The chemometric results for flow injection mass spectrometry and proton nuclear magnetic resonance spectrometry agreed well and showed similar agreement throughout the study. DNA sequencing using DNA sequence data from two independent gene regions confirmed the metabolic fingerprinting results. Differences were observed between A. racemosa samples from four different sources, although the variance within species was still significantly less than the variance between species. A model based on the combined A. racemosa samples from the four sources consistently permitted distinction between species. Additionally, the combined A. racemosa samples were distinguishable from commercial root samples and from commercial supplements in tablet, capsule, or liquid form. DNA sequencing verified the lack of authenticity of the commercial roots but was unsuccessful in characterizing many of the supplements due to the lack of available DNA. C1 [Harnly, James; Chen, Pei; Sun, Jianghao; Huang, Huilian] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Colson, Kimberly L.; Yuk, Jimmy] Bruker BioSpin, Billerica, MA USA. [McCoy, Joe-Ann H.] North Carolina Arboretum Germplasm Repository, Asheville, NC USA. [Reynaud, Danica T. Harbaugh] AuthenTechnol LLC, Richmond, CA USA. [Harrington, Peter B.] Ohio Univ, Ctr Intelligent Chem Instrumentat, Dept Chem & Biochem, Clippinger Labs, Athens, OH 45701 USA. [Fletcher, Edward J.] Strateg Sourcing Inc, Banner Elk, NC USA. RP Harnly, J (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, 10300 Baltimore Ave,Bldg 161, Beltsville, MD 20705 USA. EM james.harnly@ars.usda.gov RI Sun, Jianghao/A-6134-2010 FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements of the National Institutes of Health FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health. NR 34 TC 3 Z9 3 U1 5 U2 7 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD FEB PY 2016 VL 82 IS 3 BP 250 EP 262 DI 10.1055/s-0035-1558113 PG 13 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA DD5FM UT WOS:000369948100011 PM 26692457 ER PT J AU Shafer, ABA Wolf, JBW Alves, PC Bergstrom, L Colling, G Dalen, L De Meester, L Ekblom, R Fior, S Hajibabaei, M Hoezel, AR Hoglund, J Jensen, EL Krutzen, M Norman, AJ Osterling, EM Ouborg, NJ Piccolo, J Primmer, CR Reed, FA Roumet, M Salmona, J Schwartz, MK Segelbacher, G Thaulow, J Valtonen, M Vergeer, P Weissensteiner, M Wheat, CW Vila, C Zielinski, P AF Shafer, Aaron B. A. Wolf, Jochen B. W. Alves, Paulo C. Bergstrom, Linnea Colling, Guy Dalen, Love De Meester, Luc Ekblom, Robert Fior, Simone Hajibabaei, Mehrdad Hoezel, A. Rus Hoglund, Jacob Jensen, Evelyn L. Kruetzen, Michael Norman, Anita J. Osterling, E. Martin Ouborg, N. Joop Piccolo, John Primmer, Craig R. Reed, Floyd A. Roumet, Marie Salmona, Jordi Schwartz, Michael K. Segelbacher, Gernot Thaulow, Jens Valtonen, Mia Vergeer, Philippine Weissensteiner, Matthias Wheat, Christopher W. Vila, Carlese Zielinski, Piotr TI Genomics in Conservation: Case Studies and Bridging the Gap between Data and Application Reply SO TRENDS IN ECOLOGY & EVOLUTION LA English DT Letter C1 [Shafer, Aaron B. A.; Wolf, Jochen B. W.; Bergstrom, Linnea; Ekblom, Robert; Hoglund, Jacob; Weissensteiner, Matthias] Uppsala Univ, Ecol & Genet, Evolutionary Biol Ctr, S-75236 Uppsala, Sweden. [Alves, Paulo C.] Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, P-4485661 Oporto, Portugal. [Alves, Paulo C.] Fac Ciencias, P-4485661 Oporto, Portugal. [Colling, Guy] Musee Natl Hist Nat Luxembourg, Populat Biol, L-2160 Luxembourg, Luxembourg. [Dalen, Love] Swedish Museum Nat Hist, Bioinformat & Genet, S-10405 Stockholm, Sweden. [De Meester, Luc] KU Leuven Univ Leuven, Aquat Ecol Evolut & Conservat, B-3000 Leuven, Belgium. [Fior, Simone; Roumet, Marie] Swiss Fed Inst Technol, Integrat Biol, CH-8092 Zurich, Switzerland. [Hajibabaei, Mehrdad] Univ Guelph, Integrat Biol, Guelph, ON N1G 2W1, Canada. [Hoezel, A. Rus] Univ Durham, Biol & Biomed Sci, Durham DH1 3LE, England. [Jensen, Evelyn L.] Univ British Columbia Okanagan, Biol, Kelowna, BC V1V 1V7, Canada. [Kruetzen, Michael] Univ Zurich, Anthropol Inst & Museum, CH-8057 Zurich, Switzerland. [Norman, Anita J.] Swedish Univ Agr Sci, Wildlife Fish & Environm Studies, S-90183 Umea, Sweden. [Osterling, E. Martin] Karlstad Univ, Biol, S-65188 Karlstad, Sweden. [Ouborg, N. Joop] Radboud Univ Nijmegen, Expt Plant Ecol, NL-6500 GL Nijmegen, Netherlands. [Primmer, Craig R.] Univ Turku, Biol, Turku 20014, Finland. [Reed, Floyd A.] Univ Hawaii Manoa, Biol, Honolulu, HI 96822 USA. [Salmona, Jordi] Inst Gulbenkian Ciencias, Populat & Conservat Genet Grp, P-2780156 Oeiras, Portugal. [Schwartz, Michael K.] USDA, Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Segelbacher, Gernot] Univ Freiburg, Wildlife Ecol & Management, D-79106 Freiburg, Germany. [Thaulow, Jens] Norwegian Inst Water Res, Freshwater Biol, N-0349 Oslo, Norway. [Valtonen, Mia] Univ Eastern Finland, Biol, Joensuu 80101, Finland. [Vergeer, Philippine] Wageningen Univ, Nat Conservat & Plant Ecol, NL-6708 PB Wageningen, Netherlands. [Wheat, Christopher W.] Stockholm Univ, Zool, S-10691 Stockholm, Sweden. [Vila, Carlese] Estn Biol Donana, Conservat & Evolutionary Genet Grp, Seville 41092, Spain. [Zielinski, Piotr] Jagiellonian Univ, Inst Environm Sci, PL-30387 Krakow, Poland. RP Shafer, ABA; Wolf, JBW (reprint author), Uppsala Univ, Ecol & Genet, Evolutionary Biol Ctr, S-75236 Uppsala, Sweden. EM aaron.shafer@ebc.uu.se; jochen.wolf@ebc.uu.se RI Ouborg, Joop/C-1028-2011; Vila, Carles/H-4893-2013; CSIC, EBD Donana/C-4157-2011; ALVES, Paulo Celio/B-5448-2009; Primmer, Craig/B-8179-2008; De Meester, Luc/F-3832-2015; Dalen, Love/A-6686-2008 OI Vila, Carles/0000-0002-4206-5246; CSIC, EBD Donana/0000-0003-4318-6602; ALVES, Paulo Celio/0000-0003-4797-0939; Primmer, Craig/0000-0002-3687-8435; De Meester, Luc/0000-0001-5433-6843; Dalen, Love/0000-0001-8270-7613 NR 8 TC 5 Z9 5 U1 22 U2 53 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0169-5347 J9 TRENDS ECOL EVOL JI Trends Ecol. Evol. PD FEB PY 2016 VL 31 IS 2 BP 83 EP 84 DI 10.1016/j.tree.2015.11.010 PG 2 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA DC8IF UT WOS:000369462400002 PM 26704456 ER PT J AU Mollov, D Maroon-Lango, C Kuniata, L AF Mollov, Dimitre Maroon-Lango, Clarissa Kuniata, Lastus TI Detection by next generation sequencing of a multi-segmented viral genome from sugarcane associated with Ramu stunt disease SO VIRUS GENES LA English DT Article DE Ramu stunt disease; Ramu stunt virus; Tenuivirus; Multi-segmented virus genome; Negative strand ssRNA virus; Next generation sequencing ID YELLOW STRIPE VIRUS; HOJA-BLANCA-VIRUS; PAPUA-NEW-GUINEA; GENUS TENUIVIRUS; NUCLEOTIDE-SEQUENCE; VECTOR AB Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. Sugarcane cv. Ragnar from Papua New Guinea with symptoms of Ramu stunt was analyzed by next generation sequencing. Total RNA was extracted and whole transcriptome shotgun sequencing was performed using an Illumina platform. Over thirty-seven million reads with an average length of 100 nucleotides were obtained. More than eighteen thousand contigs were assembled and subjected to BLASTX analysis. Twenty-one contigs were virus related and six were associated with plant viruses. The BLAST algorithms revealed sequence similarity to Tenuivirus and Phlebovirus, genera of viruses whose members contain genomes consisting of multiple RNA segments. The six contigs derived from the RNA sequencing data correspond to six RNAs that compose the Ramu stunt virus genome. Primers were designed for each of the six RNAs and RT-PCR amplicons were obtained only from the symptomatic sugarcane. There was concordance between the sequence data of the contigs obtained from the NGS and that of the amplicons obtained by RT-PCR. The NGS approach allowed us to determine the complete genomic sequence of Ramu stunt virus. It is likely that this virus is the causal agent of Ramu stunt disease. C1 [Mollov, Dimitre] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD USA. [Maroon-Lango, Clarissa] USDA APHIS PPQ Plant Hlth Programs, Riverdale, MD USA. [Kuniata, Lastus] Ramu Agri Ind, New Britain Palm Oil, Lae, Morobe Province, Papua N Guinea. RP Mollov, D (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD USA. EM dimitre.mollov@ars.usda.gov NR 22 TC 0 Z9 0 U1 5 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD FEB PY 2016 VL 52 IS 1 BP 152 EP 155 DI 10.1007/s11262-015-1279-5 PG 4 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA DD6YK UT WOS:000370070400020 PM 26728080 ER PT J AU Hong, EM Nam, WH Choi, JY Pachepsky, YA AF Hong, Eun-Mi Nam, Won-Ho Choi, Jin-Yong Pachepsky, Yakov A. TI Projected irrigation requirements for upland crops using soil moisture model under climate change in South Korea SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Climate change; Evapotranspiration; Irrigation requirement; Soil moisture model; South Korea; Upland crop ID WATER-BALANCE; REFERENCE EVAPOTRANSPIRATION; COEFFICIENT METHOD; CROPPING SYSTEMS; NEXT-GENERATION; CHANGE IMPACTS; NORTH CHINA; RIVER-BASIN; DROUGHT; REGION AB An increase in abnormal climate change patterns and unsustainable irrigation in uplands cause drought and affect agricultural water security, crop productivity, and price fluctuations. In this study, we developed a soil moisture model to project irrigation requirements (IR) for upland crops under climate change using estimated effective rainfall (ER), crop evapotranspiration (ETc) and the IR of 29 major upland crops in South Korea. The temperature and precipitation will increase, but the ER is projected to decrease under climate change. ETc and the net irrigation requirement (NIR) are expected to increase under climate change. Vegetable crops have less ER and more NIR than cereal crops with a similar amount of ETc, which means they are more sensitive to water scarcity and IR than cereal crops. In addition, we found that barley has the smallest daily ETc and IR but the highest increase rate in NIR under climate change, especially in the central part of South Korea. The NIR of Chinese cabbage-fall is the lowest in the northern region and increases moving southwards. The NIR of spinach is projected to increase gradually from the southern and eastern coastlines to the northern inland area. Onions have the largest ETc and NIR of the 29 upland crops, but they show small changes compared to other crops under climate change. Water scarcity is a major limiting factor for sustainable agricultural production. The variation of IR and ETc values for each crop under different climate change scenarios depends on the crop, soil, space, and meteorological characteristics. The results of this study can be used as a guideline for irrigation and soil water management for upland crops under climate change. (C) 2015 Elsevier B.V. All rights reserved. C1 [Hong, Eun-Mi; Pachepsky, Yakov A.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD USA. [Hong, Eun-Mi] Oak Ridge Inst Sci & Engn, Oak Ridge, TN USA. [Nam, Won-Ho] Univ Nebraska, Sch Nat Resources, Natl Drought Mitigat Ctr, Lincoln, NE USA. [Choi, Jin-Yong] Seoul Natl Univ, Dept Rural Syst Engn, Seoul, South Korea. [Choi, Jin-Yong] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul, South Korea. RP Nam, WH (reprint author), Univ Nebraska, Sch Nat Resources, Natl Drought Mitigat Ctr, Lincoln, NE USA. EM wonho.nam@gmail.com RI NAM, WONHO/B-3489-2014; OI NAM, WONHO/0000-0002-9671-6569; Pachepsky, Yakov/0000-0003-0232-6090 FU DOE [DE-AC05-060R23100]; Basic Science Research Program through the National Research Foundation of Korea (NRF); Ministry of Education, Science and Technology [2013R1A6A3A03019009] FX This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-AC05-060R23100. Also, this research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and was funded by the Ministry of Education, Science and Technology (2013R1A6A3A03019009). All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of USDA, ARS, DOE, ORAU/ORISE, NRF or any of its sub-agencies. Finally, the authors would like to thank the editor, and anonymous reviewers who took the time to review and provide guidance on this paper. NR 71 TC 1 Z9 1 U1 2 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD FEB PY 2016 VL 165 BP 163 EP 180 DI 10.1016/j.agwat.2015.12.003 PG 18 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DC4PT UT WOS:000369203500016 ER PT J AU Ma, YY Follis, JL Smith, CE Tanaka, T Manichaikul, AW Chu, AY Samieri, C Zhou, X Guan, WH Wang, L Biggs, ML Chen, YDI Hernandez, DG Borecki, I Chasman, DI Rich, SS Ferrucci, L Irvin, MR Aslibekyan, S Zhi, DG Tiwari, HK Claas, SA Sha, J Kabagambe, EK Lai, CQ Parnell, LD Lee, YC Amouyel, P Lambert, JC Psaty, BM King, IB Mozaffarian, D McKnight, B Bandinelli, S Tsai, MY Ridker, PM Ding, JZ Mstat, KL Liu, YM Sotoodehnia, N Barberger-Gateau, P Steffen, LM Siscovick, DS Absher, D Arnett, DK Ordovas, JM Lemaitre, RN AF Ma, Yiyi Follis, Jack L. Smith, Caren E. Tanaka, Toshiko Manichaikul, Ani W. Chu, Audrey Y. Samieri, Cecilia Zhou, Xia Guan, Weihua Wang, Lu Biggs, Mary L. Chen, Yii-Der I. Hernandez, Dena G. Borecki, Ingrid Chasman, Daniel I. Rich, Stephen S. Ferrucci, Luigi Irvin, Marguerite Ryan Aslibekyan, Stella Zhi, Degui Tiwari, Hemant K. Claas, Steven A. Sha, Jin Kabagambe, Edmond K. Lai, Chao-Qiang Parnell, Laurence D. Lee, Yu-Chi Amouyel, Philippe Lambert, Jean-Charles Psaty, Bruce M. King, Irena B. Mozaffarian, Dariush McKnight, Barbara Bandinelli, Stefania Tsai, Michael Y. Ridker, Paul M. Ding, Jingzhong Mstat, Kurt Lohmant Liu, Yongmei Sotoodehnia, Nona Barberger-Gateau, Pascale Steffen, Lyn M. Siscovick, David S. Absher, Devin Arnett, Donna K. Ordovas, Jose M. Lemaitre, Rozenn N. TI Interaction of methylation-related genetic variants with circulating fatty acids on plasma lipids: a meta-analysis of 7 studies and methylation analysis of 3 studies in the Cohorts for Heart and Aging Research in Genomic Epidemiology consortium SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE DNA methylation; epidemiology; fatty acids; genetic variants; plasma lipids ID CORONARY-ARTERY-DISEASE; DNA METHYLATION; WIDE ASSOCIATION; TANGIER-DISEASE; CARDIOVASCULAR-DISEASE; LOWERING DRUGS; DIET NETWORK; BLOOD-LIPIDS; SINGLE CPG; LOCI AB Background: DNA methylation is influenced by diet and single nucleotide polymorphisms (SNPs), and methylation modulates gene expression. Objective: We aimed to explore whether the gene-by-diet interactions on blood lipids act through DNA methylation. Design: We selected 7 SNPs on the basis of predicted relations in fatty acids, methylation, and lipids. We conducted a meta-analysis and a methylation and mediation analysis with the use of data from the CHARGE (Cohorts for Heart and Aging Research in Genomic Epidemiology) consortium and the ENCODE (Encyclopedia of DNA Elements) consortium. Results: On the basis of the meta-analysis of 7 cohorts in the CHARGE consortium, higher plasma HDL cholesterol was associated with fewer C alleles at ATP-binding cassette subfamily A member 1 (ABCA1) rs2246293 (beta =-0.6 mg/dL, P = 0.015) and higher circulating eicosapentaenoic acid (EPA) (beta = 3.87 mg/dL, P = 5.62 X 1021). The difference in HDL cholesterol associated with higher circulating EPA was dependent on genotypes at rs2246293, and it was greater for each additional C allele (beta = 1.69 mg/dL, P = 0.006). In the GOLDN (Genetics of Lipid Lowering Drugs and Diet Network) study, higher ABCA1 promoter cg14019050 methylation was associated with more C alleles at rs2246293 (beta = 8.84%, P = 3.51 x 10(18)) and lower circulating EPA (p =-1.46%, P = 0.009), and the mean difference in methylation of cg14019050 that was associated with higher EPA was smaller with each additional C allele of rs2246293 =-2.83%, P = 0.007). Higher ABCA1 cg14019050 methylation was correlated with lower ABCA1 expression (r =-0.61, P = 0.009) in the ENCODE consortium and lower plasma HDL cholesterol in the GOLDN study (r =-0.12, P = 0.0002). An additional mediation analysis was meta-analyzed across the GOLDN study, Cardiovascular Health Study, and the Multi-Ethnic Study of Atherosclerosis. Compared with the model without the adjustment of cg14019050 methylation, the model with such adjustment provided smaller estimates of the mean plasma HDL cholesterol concentration in association with both the rs2246293 C allele and EPA and a smaller difference by rs2246293 genotypes in the EPA-associated HDL cholesterol. However, the differences between 2 nested models were NS (P > 0.05). Conclusion: We obtained little evidence that the gene-by-fatty acid interactions on blood lipids act through DNA methylation. C1 [Ma, Yiyi] Boston Univ, Dept Med, Biomed Genet, Boston, MA 02215 USA. [Ma, Yiyi; Smith, Caren E.; Lai, Chao-Qiang; Parnell, Laurence D.; Lee, Yu-Chi; Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Mozaffarian, Dariush] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Follis, Jack L.] Univ St Thomas, Dept Math Comp Sci & Cooperat Engn, Houston, TX 77006 USA. [Tanaka, Toshiko; Ferrucci, Luigi] NIA, Translat Gerontol Branch, Bethesda, MD 20892 USA. [Hernandez, Dena G.] NIA, Lab Neurogenet, Bethesda, MD 20892 USA. [Manichaikul, Ani W.; Rich, Stephen S.] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA USA. [Chu, Audrey Y.; Wang, Lu; Chasman, Daniel I.; Ridker, Paul M.] Harvard Univ, Brigham & Womens Hosp, Sch Med, Boston, MA 02115 USA. [Samieri, Cecilia; Barberger-Gateau, Pascale] Univ Bordeaux, INSERM, U897, Bordeaux, France. [Samieri, Cecilia; Barberger-Gateau, Pascale] Univ Bordeaux, ISPED, Bordeaux, France. [Zhou, Xia; Steffen, Lyn M.] Univ Minnesota, Div Epidemiol & Community Hlth, Sch Publ Hlth, Minneapolis, MN USA. [Guan, Weihua] Univ Minnesota, Div Biostat, Sch Publ Hlth, Minneapolis, MN USA. [Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN USA. [Biggs, Mary L.; McKnight, Barbara] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Psaty, Bruce M.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Psaty, Bruce M.] Univ Washington, Dept Hlth Serv, Seattle, WA 98195 USA. [Biggs, Mary L.; Psaty, Bruce M.; McKnight, Barbara; Sotoodehnia, Nona; Lemaitre, Rozenn N.] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA USA. [Chen, Yii-Der I.] Cedars Sinai Med Ctr, Med Genet Res Inst, Los Angeles, CA 90048 USA. [Chen, Yii-Der I.] Harbor Univ Calif, Los Angeles Med Ctr, Los Angeles Biomed Inst, Torrance, CA USA. [Borecki, Ingrid] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Irvin, Marguerite Ryan; Aslibekyan, Stella; Claas, Steven A.; Sha, Jin; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Zhi, Degui; Tiwari, Hemant K.] Univ Alabama Birmingham, Dept Biostat, Birmingham, AL 35294 USA. [Kabagambe, Edmond K.] Vanderbilt Univ, Dept Med, Nashville, TN USA. [Amouyel, Philippe; Lambert, Jean-Charles] INSERM, UMER1167, F-59045 Lille, France. [Amouyel, Philippe; Lambert, Jean-Charles] Univ Lille, Lille, France. [Amouyel, Philippe; Lambert, Jean-Charles] Inst Pasteur, Lille, France. [Amouyel, Philippe] Reg Univ Hosp Lille, Lille, France. [King, Irena B.] Univ New Mexico, Dept Internal Med, Albuquerque, NM 87131 USA. [Bandinelli, Stefania] Azienda Sanitaria Firenze, Geriatr Unit, Florence, Italy. [Ding, Jingzhong] Wake Forest Sch Med, Dept Internal Med, Winston Salem, NC USA. [Mstat, Kurt Lohmant; Liu, Yongmei] Wake Forest Sch Med, Dept Epidemiol & Prevent Publ Hlth Sci, Winston Salem, NC USA. [Siscovick, David S.] New York Acad Med, New York, NY USA. [Absher, Devin] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [Ordovas, Jose M.] Cardiovasc Res Ctr, Dept Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, Jose M.] IMDEA Food Inst, Madrid, Spain. RP Ma, YY (reprint author), Boston Univ, Dept Med, Biomed Genet, Boston, MA 02215 USA. EM yiyima@bu.edu RI Lambert, jean-charles/A-9553-2014; Samieri, Cecilia/E-3334-2014; OI Lambert, jean-charles/0000-0003-0829-7817; Samieri, Cecilia/0000-0001-9809-7506; Claas, Steven/0000-0001-9789-8395 FU NIH through the American Recovery and Reinvestment Act [5RC2HL102419]; National Heart, Lung, and Blood Institute (NHLBI) [U01HL07252A-04, 5R01HL1043135-04]; National Institute of Neurological Disorders and Stroke [T32NS054584]; NHLBI [HL092111, HL111089, HL116747, 1R01HL101250-01]; Laughlin Family; Alpha Phi Foundation; Locke Charitable Foundation FX Supported by the NIH through the American Recovery and Reinvestment Act of 2009 (5RC2HL102419; to the "Building on GWAS for NHLBI-diseases: the U.S. CHARGE Consortium"). The methylation analysis in the Genetics of Lipid Lowering Drugs and Diet Network was funded by the National Heart, Lung, and Blood Institute (NHLBI) (grants U01HL07252A-04 and 5R01HL1043135-04) and by the National Institute of Neurological Disorders and Stroke (grant T32NS054584). Methylation in the Cardiovascular Health Study was funded by the NHLBI (grants HL092111, HL111089, and HL116747) and by the Laughlin Family, Alpha Phi Foundation, and Locke Charitable Foundation. The methylation analysis the in Multi-Ethnic Study of Atherosclerosis was supported by the NHLBI (grant 1R01HL101250-01; to Wake Forest University Health Sciences). Study-specific sources of support and acknowledgments are listed in Supplemental Methods. NR 49 TC 0 Z9 0 U1 2 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 2016 VL 103 IS 2 BP 567 EP 578 DI 10.3945/ajcn.115.112987 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC8JJ UT WOS:000369465400033 PM 26791180 ER PT J AU Gese, EM Roberts, BM Knowlton, FF AF Gese, Eric M. Roberts, Beth M. Knowlton, Frederick F. TI Nutritional effects on reproductive performance of captive adult female coyotes (Canis latrans) SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Canis latrans; Coyote; Litter; Nutrition; Reproduction; Pup survival ID FEED RESTRICTION; OVULATION RATE; LITTER SIZE; POPULATION; ECOLOGY; DEMOGRAPHY; BEHAVIOR; DECLINE; GILTS AB Interactions between animals and their environment are fundamental to ecological research. Field studies of coyote (Canis latrans) reproductive performance suggest mean litter size changes in response to prey abundance. However, this relationship has been assessed primarily by using carcasses collected from trappers. The objective of this study was to assess whether nutritional manipulation prior to mating affected reproduction in adult female coyotes. We examined the effects of caloric restriction during the 7 months prior to estrus on the reproductive rates of 11 captive female coyotes and the subsequent initial survival of pups through two reproductive cycles. This was a 2-year study with a cross-over design so each female was monitored for reproductive performance on each of the two diet treatments. We assessed the number of implantation scars, number of pups born, sex ratios of pups, average pup weight at birth and 2- and 6-weeks of age, and the survival rates between implantation and 2-weeks of age for two diet treatments. We found the mean number of implantation sites and pups whelped during a reproductive cycle was influenced by food-intake prior to conception. Additionally, we found evidence suggesting the effects of nutritional stress may persist for additional breeding cycles. We also provided evidence suggesting well-fed females tended to have more male pups. Understanding how environmental factors influence reproductive output may improve model predictions of coyote population dynamics. Published by Elsevier B.V. C1 [Gese, Eric M.; Knowlton, Frederick F.] Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Dept Wildland Resources, Logan, UT 84322 USA. [Roberts, Beth M.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Roberts, Beth M.] Memphis Zoo, 2000 Prentiss Pl, Memphis, TN 38112 USA. RP Gese, EM (reprint author), Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Dept Wildland Resources, Logan, UT 84322 USA. EM eric.gese@usu.edu FU U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Logan Field Station, Utah FX Funding and logistical support provided by the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Logan Field Station, Utah. We thank K. Grant for her care and diligence in hand-rearing all coyotes used in this study, and the staff and students at the Millville Predator Research Facility, especially S. Brummer, T. Christensen, P. Darrow, L. Gilbert-Norton, J. Schultz, and E. Stevenson for assistance with animal care and observations. NR 40 TC 1 Z9 1 U1 7 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 EI 1873-2232 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD FEB PY 2016 VL 165 BP 69 EP 75 DI 10.1016/j.anireprosci.2015.12.009 PG 7 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA DC9TE UT WOS:000369563100009 PM 26763531 ER PT J AU Richards, GP Fay, JP Uknalis, J Olanya, OM Watson, MA AF Richards, Gary P. Fay, Johnna P. Uknalis, Joseph Olanya, O. Modesto Watson, Michael A. TI Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BDELLOVIBRIO-BACTERIOVORUS 109J; VIBRIO-PARAHAEMOLYTICUS; NONCULTURABLE STATE; RESUSCITATION; PATHOGENS; VULNIFICUS; ORGANISMS; VANNAMEI; CULTURE; CELLS AB Halobacteriovorax (formerly Bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. Four strains of Halobacteriovorax originally isolated in Vibrio parahaemolyticus O3:K6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. This technique was essential, since 0.45-mu m filtration alone was unable to remove infectious Vibrio minicells, as determined by scanning electron microscopy and cultural methods. Purified Halobacteriovorax strains were screened for predation against other V. parahaemolyticus strains and against Vibrio vulnificus, Vibrio alginolyticus, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium DT104, all potential threats to seafood safety. They showed high host specificity and were predatory only against strains of V. parahaemolyticus. In addition, strains of Halobacteriovorax that were predatory for E. coli O157:H7 and S. Typhimurium DT104 were isolated from a tidal river at 5 ppt salinity. In a modified plaque assay agar, they killed their respective prey over a broad range of salinities (5 to 30 ppt). Plaques became smaller as the salinity levels rose, suggesting that the lower salinities were optimal for the predators' replication. These species also showed broader host specificity, infectious against each other's original hosts as well as against V. parahaemolyticus strains. In summary, this study characterized strains of Halobacteriovorax which may be considered for use in the development of broad-based biocontrol technologies to enhance the safety of commercially marketed shellfish and other foods. C1 [Richards, Gary P.; Watson, Michael A.] Delaware State Univ, ARS, USDA, Dover, DE USA. [Fay, Johnna P.] Univ Delaware, Dept Anim & Food Sci, Newark, DE USA. [Uknalis, Joseph; Olanya, O. Modesto] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. RP Richards, GP (reprint author), Delaware State Univ, ARS, USDA, Dover, DE USA. EM gary.richards@ars.usda.gov FU USDA ARS under CRIS project [8072-42000-065-00D] FX Support for this study was provided by intramural funding from USDA ARS under CRIS project 8072-42000-065-00D (G.P.R.). NR 31 TC 1 Z9 1 U1 3 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2016 VL 82 IS 3 BP 922 EP 927 DI 10.1128/AEM.03136-15 PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DC7BR UT WOS:000369373800016 ER PT J AU Bergholz, TM den Bakker, HC Katz, LS Silk, BJ Jackson, KA Kucerova, Z Joseph, LA Turnsek, M Gladney, LM Halpin, JL Xavier, K Gossack, J Ward, TJ Frace, M Tarrc, CL AF Bergholz, Teresa M. den Bakker, Henk C. Katz, Lee S. Silk, Benjamin J. Jackson, Kelly A. Kucerova, Zuzana Joseph, Lavin A. Turnsek, Maryann Gladney, Lori M. Halpin, Jessica L. Xavier, Karen Gossack, Joseph Ward, Todd J. Frace, Michael Tarrc, Cheryl L. TI Determination of Evolutionary Relationships of Outbreak-Associated Listeria monocytogenes Strains of Serotypes 1/2a and 1/2b by Whole-Genome Sequencing SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID SINGLE NUCLEOTIDE POLYMORPHISMS; MOBILE GENETIC ELEMENTS; MULTISTATE OUTBREAK; UNITED-STATES; INVASIVE LISTERIOSIS; MAXIMUM-LIKELIHOOD; PASTEURIZED MILK; EPIDEMIC CLONES; GASTROENTERITIS; SURVEILLANCE AB We used whole-genome sequencing to determine evolutionary relationships among 20 outbreak-associated clinical isolates of Listeria monocytogenes serotypes 1/2a and 1/2b. Isolates from 6 of 11 outbreaks fell outside the clonal groups or "epidemic clones" that have been previously associated with outbreaks, suggesting that epidemic potential may be widespread in L. monocytogenes and is not limited to the recognized epidemic clones. Pairwise comparisons between epidemiologically related isolates within clonal complexes showed that genome-level variation differed by 2 orders of magnitude between different comparisons, and the distribution of point mutations (core versus accessory genome) also varied. In addition, genetic divergence between one closely related pair of isolates from a single outbreak was driven primarily by changes in phage regions. The evolutionary analysis showed that the changes could be attributed to horizontal gene transfer; members of the diverse bacterial community found in the production facility could have served as the source of novel genetic material at some point in the production chain. The results raise the question of how to best utilize information contained within the accessory genome in outbreak investigations. The full magnitude and complexity of genetic changes revealed by genome sequencing could not be discerned from traditional subtyping methods, and the results demonstrate the challenges of interpreting genetic variation among isolates recovered from a single outbreak. Epidemiological information remains critical for proper interpretation of nucleotide and structural diversity among isolates recovered during outbreaks and will remain so until we understand more about how various population histories influence genetic variation. C1 [Bergholz, Teresa M.] N Dakota State Univ, Fargo, ND 58105 USA. [den Bakker, Henk C.] Cornell Univ, Ithaca, NY USA. [Katz, Lee S.; Silk, Benjamin J.; Jackson, Kelly A.; Kucerova, Zuzana; Joseph, Lavin A.; Turnsek, Maryann; Gladney, Lori M.; Halpin, Jessica L.; Frace, Michael; Tarrc, Cheryl L.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Xavier, Karen; Gossack, Joseph] Colorado Dept Publ Hlth & Environm, Denver, CO USA. [Ward, Todd J.] ARS, USDA, Peoria, IL USA. [den Bakker, Henk C.] Texas Tech Univ, Lubbock, TX 79409 USA. RP Tarrc, CL (reprint author), Ctr Dis Control & Prevent, Atlanta, GA USA. EM ctarr@cdc.gov RI Bergholz, Teresa/A-2815-2010 OI Bergholz, Teresa/0000-0002-3976-5858 FU USDA NIFA Special Research Grants [2008-34459-19043, 2009-34459-19750] FX T.M.B. and H.C.D.B. thank M. Wiedmann for support from USDA NIFA Special Research Grants 2008-34459-19043 and 2009-34459-19750. NR 54 TC 8 Z9 8 U1 3 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2016 VL 82 IS 3 BP 928 EP 938 DI 10.1128/AEM.02440-15 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DC7BR UT WOS:000369373800017 PM 26590286 ER PT J AU Carter, MQ Quinones, B He, XH Zhong, W Louie, JW Lee, BG Yambao, JC Mandrell, RE Cooley, MB AF Carter, Michelle Qiu Quinones, Beatriz He, Xiaohua Zhong, Wayne Louie, Jacqueline W. Lee, Bertram G. Yambao, Jaszemyn C. Mandrell, Robert E. Cooley, Michael B. TI An Environmental Shiga Toxin-Producing Escherichia coli O145 Clonal Population Exhibits High-Level Phenotypic Variation That Includes Virulence Traits SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HEMOLYTIC-UREMIC-SYNDROME; SPINACH-ASSOCIATED OUTBREAK; UNITED-STATES; PATHOADAPTIVE MUTATIONS; 2 GENE; CONVERTING BACTERIOPHAGES; COMPARATIVE GENOMICS; BACTERIAL PATHOGENS; BACILLUS-SUBTILIS; CURLI VARIANTS AB Shiga toxin-producing Escherichia coli (STEC) serotype O145 is one of the major non-O157 serotypes associated with severe human disease. Here we examined the genetic diversity, population structure, virulence potential, and antimicrobial resistance profiles of environmental O145 strains recovered from a major produce production region in California. Multilocus sequence typing analyses revealed that sequence type 78 (ST-78), a common ST in clinical strains, was the predominant genotype among the environmental strains. Similarly, all California environmental strains belonged to H28, a common H serotype in clinical strains. Although most environmental strains carried an intact fliC gene, only one strain retained swimming motility. Diverse stx subtypes were identified, including stx(1a), stx(2a), stx(2c), and stx(2e). Although no correlation was detected between the stx genotype and Stx1 production, high Stx2 production was detected mainly in strains carrying stx(2a) only and was correlated positively with the cytotoxicity of Shiga toxin. All environmental strains were capable of producing enterohemolysin, whereas only 10 strains were positive for anaerobic hemolytic activity. Multidrug resistance appeared to be common, as nearly half of the tested O145 strains displayed resistance to at least two different classes of antibiotics. The core virulence determinants of enterohemorrhagic E. coli were conserved in the environmental STEC O145 strains; however, there was large variation in the expression of virulence traits among the strains that were highly related genotypically, implying a trend of clonal divergence. Several cattle isolates exhibited key virulence traits comparable to those of the STEC O145 outbreak strains, emphasizing the emergence of hypervirulent strains in agricultural environments. C1 [Carter, Michelle Qiu; Quinones, Beatriz; Zhong, Wayne; Louie, Jacqueline W.; Lee, Bertram G.; Yambao, Jaszemyn C.; Mandrell, Robert E.; Cooley, Michael B.] ARS, Prod Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA USA. [He, Xiaohua] ARS, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, USDA, Albany, CA USA. RP Carter, MQ (reprint author), ARS, Prod Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA USA. EM michelle.carter@ars.usda.gov FU USDA \ Agricultural Research Service (ARS) [5325-42000-046-00D, 5325-42000-047-00D, 5325-42000-048-00D] FX USDA vertical bar Agricultural Research Service (ARS) provided funding to Michelle Qiu Carter and Michael B. Cooley under grant number 5325-42000-046-00D. USDA vertical bar Agricultural Research Service (ARS) provided funding to Beatriz Quinones under grant number 5325-42000-047-00D. USDA vertical bar Agricultural Research Service (ARS) provided funding to Xiaohua He under grant number 5325-42000-048-00D. NR 78 TC 2 Z9 2 U1 1 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2016 VL 82 IS 4 BP 1090 EP 1101 DI 10.1128/AEM.03172-15 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DC7CJ UT WOS:000369375900012 PM 26637597 ER PT J AU Powell, LL Wolfe, JD Johnson, EI Stouffer, PC AF Powell, Luke L. Wolfe, Jared D. Johnson, Erik I. Stouffer, Philip C. TI Forest recovery in post-pasture Amazonia: Testing a conceptual model of space use by insectivorous understory birds SO BIOLOGICAL CONSERVATION LA English DT Article DE Antthrush; Fragmentation; Home range; Secondary forest; Telemetry; Woodcreeper ID ANIMAL MOVEMENT PATHS; RAIN-FOREST; BRAZILIAN AMAZONIA; FRACTAL DIMENSION; HOME-RANGE; GENE FLOW; FRAGMENTS; SECONDARY; DEFORESTATION; DISTRIBUTIONS AB Understanding how animals move in a complex habitat mosaic is critical to biodiversity conservation as deforested lands and secondary rainforests accumulate in landscapes previously dominated by primary forests. To visualize the spatiotemporal dynamics of secondary forest recovery after pasture abandonment, we formed a conceptual model predicting avian space use during a temporal sequence beginning with deforestation and continuing through regrowth of old secondary forest. We tested five predictions of the model at the Biological Dynamics of Forest Fragments Project near Manaus, Brazil, using 73 radio-tagged understory insectivores of three species: two woodcreepers (Glyphorynchus spirurus, Xiphorhynchus pardalotus) and a terrestrial antthrush (Formicarius colma). Both woodcreepers provided evidence to support all predictions except that of greater path tortuosity in primary forest. Woodcreepers using secondary forest had larger home ranges, larger core areas, and faster movement rates than in primary forest Further, the proportion of all species' core areas in primary forest exceeded the proportion of home ranges in primary forest. Formicarius colma showed a fundamentally different pattern than the woodcreepers: it essentially avoided secondary forest until 27-31 years after pasture abandonment, at which point movements were indistinguishable from those in primary forest. Formicarius colma and other terrestrial insectivores show implastic spatiotemporal responses to recovering secondary growth, which we suspect contributes to the sensitivity of this guild to forest disturbance. Quantifying the value of marginal (and economically inconsequential) habitats such as secondary forest will be essential as land managers strive to maintain species persistence and connectivity in increasingly heterogeneous tropical landscapes. Published by Elsevier Ltd. C1 Inst Nacl de Pesquisas da Amazonia, Biol Dynam Forest Fragments Project, CP 478, BR-69011097 Manaus, AM, Brazil. Louisiana State Univ, Sch Renewable Nat Resources, RNR 227, Baton Rouge, LA 70803 USA. Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.] US Forest Serv, Redwood Sci Lab, Arcata, CA 95521 USA. [Johnson, Erik I.] Natl Audubon Soc, 6160 Perkins Rd, Baton Rouge, LA 70808 USA. RP Powell, LL (reprint author), Smithsonian Conservat Biol Inst, Migratory Bird Ctr, Natl Zoolog Pk, Washington, DC 20013 USA. EM Luke.L.Powell@gmail.com FU National Science Foundation [LTREB 0545491]; American Union of Ornithologists' Research Award; American Union of Ornithologists' Frank M. Chapman Award; Wilson Ornithological Society's Paul A Stewart Award; American Philosophical Society's Lewis Clark Fund FX LLP would like to dedicate this paper to the memory of his Master's adviser Bill Glanz, whose enthusiasm for the natural world was an inspiration to all who knew him. We thank Marconi Cerqueira, Elizabeth Condon, Paul Des Brisay, Camila Duarte, Gilberto Fernandez Arellano, Jairo Lopes, Alercio Marajo de Reis, Rachelle McLaughlin, Karl Mokross, Osmaildo, Aida Rodrigues, and Tatiana Straatmann for their contributions in the field. The LSU "Bird Lunch" group, Kristina Cockle, Kyle Harms, Jim Hines, Michael Kaller, Curtis Marantz, Michael Lefsky, Michael Patten, Scott Saleska, Stefan Woltmann, and J. Van Remsen had important contributions, including insightful reviews and advice on data analyses. James Nichols helped us analyze differences in proportions of home ranges and core areas in primary forest. We thank the following funding sources: National Science Foundation's Long Term Ecological Research grant in Biology (LTREB 0545491), American Union of Ornithologists' Research Award, the American Union of Ornithologists' Frank M. Chapman Award, the Wilson Ornithological Society's Paul A Stewart Award, and the American Philosophical Society's Lewis & Clark Fund. We conducted this research under LSU Institutional Animal Care and Use Committee approval and under applicable Brazilian permits. This is publication number 681 in the BDFFP technical series and number 40 in the Amazonian ornithology technical series. This manuscript was approved for publication by the Director of the Louisiana Agricultural Experimental Station as manuscript number 2015-241-22757. NR 63 TC 0 Z9 0 U1 8 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD FEB PY 2016 VL 194 BP 22 EP 30 DI 10.1016/j.biocon.2015.11.025 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DC8FW UT WOS:000369456300003 ER PT J AU Wilcox, TM McKelvey, KS Young, MK Sepulveda, AJ Shepard, BB Jane, SF Whiteley, AR Lowe, WH Schwartz, MK AF Wilcox, Taylor M. McKelvey, Kevin S. Young, Michael K. Sepulveda, Adam J. Shepard, Bradley B. Jane, Stephen F. Whiteley, Andrew R. Lowe, Winsor H. Schwartz, Michael K. TI Understanding environmental DNA detection probabilities: A case study using a stream-dwelling char Salvelinus fontinalis SO BIOLOGICAL CONSERVATION LA English DT Article DE eDNA; Stream; Detection; Sampling; Genetics; Fish ID 2-SPECIES OCCUPANCY MODELS; ESTIMATING SITE OCCUPANCY; REAL-TIME PCR; WATER SAMPLES; BROOK TROUT; PARTICLE-TRANSPORT; MONITORING PROGRAM; EDNA; DEPOSITION; ABUNDANCE AB Environmental DNA sampling (eDNA) has emerged as a powerful tool for detecting aquatic animals. Previous research suggests that eDNA methods are substantially more sensitive than traditional sampling. However, the factors influencing eDNA detection and the resulting sampling costs are still not well understood. Here we use multiple experiments to derive independent estimates of eDNA production rates and downstream persistence from brook trout (Salvelinus fontinalis) in streams. We use these estimates to parameterize models comparing the false negative detection rates of eDNA sampling and traditional backpack electrofishing. We find that using the protocols in this study eDNA had reasonable detection probabilities at extremely low animal densities (e.g., probability of detection 0.18 at densities of one fish per stream kilometer) and very high detection probabilities at population-level densities (e.g., probability of detection >0.99 at densities of >= 3 fish per 100 m). This is substantially more sensitive than traditional electrofishing for determining the presence of brook trout and may translate into important cost savings when animals are rare. Our findings are consistent with a growing body of literature showing that eDNA sampling is a powerful tool for the detection of aquatic species, particularly those that are rare and difficult to sample using traditional methods. (C) 2015 The Authors. Published by Elsevier Ltd. C1 [Wilcox, Taylor M.; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K.] US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Wilcox, Taylor M.; Lowe, Winsor H.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Sepulveda, Adam J.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Shepard, Bradley B.] BB Shepard & Associates, Livingston, MT 59047 USA. [Jane, Stephen F.; Whiteley, Andrew R.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Shepard, Bradley B.] Wildlife Conservat Soc, Bozeman, MT 59715 USA. RP Wilcox, TM (reprint author), US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, Missoula, MT 59801 USA. EM taylor.wilcox@umontana.edu FU NSF [DGE-1313190] FX We thank Ladd Knotek, Will Schreck, and field technicians from Montana Fish, Wildlife, and Parks, the Wildlife Conservation Society, and U.S. Geological Survey for contributing to the electrofishing sampling for the observational study. This manuscript was substantially improved by comments from three anonymous reviewers. TMW is supported by an NSF Graduate Research Fellowship (Grant No. DGE-1313190). Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government NR 51 TC 14 Z9 14 U1 35 U2 78 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD FEB PY 2016 VL 194 BP 209 EP 216 DI 10.1016/j.biocon.2015.12.023 PG 8 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DC8FW UT WOS:000369456300024 ER PT J AU Eriksen, RL Green, LA Klein, AS AF Eriksen, Renee L. Green, Lindsay A. Klein, Anita S. TI Genetic variation within and among asexual populations of Porphyra umbilicalis Kutzing (Bangiales, Rhodophyta) in the Gulf of Maine, USA SO BOTANICA MARINA LA English DT Article DE asexual evolution; EST-SSR; isolation-by-distance; population genetics ID NORTHWEST ATLANTIC; BENEFICIAL MUTATIONS; MICROSATELLITE LOCI; BREEDING SYSTEMS; LIFE-HISTORY; EVOLUTION; PLANTS; MARKERS; L.; BANGIOPHYCEAE AB The intertidal marine red alga Porphyra umbilicalis reproduces asexually in the Northwest Atlantic. We looked for population substructure among typical open-coastal and atypical estuarine habitats in seven asexual populations of P. umbilicalis from Maine to New Hampshire using eight expressed sequence tag-simple sequence repeats (EST-SSR) or microsatellite loci. Six genotypes were identified, four of which may represent recombinant genotypes from a recombination event that took place locally, or that took place prior to introduction to the Northwest Atlantic. Genotypic diversity was lowest in a population from Wiscasset, Maine, which inhabits an atypical habitat high in the intertidal zone of a bridge piling in an estuarine tidal rapid. Genotypic diversity was highest in the southernmost populations from New Hampshire; we identified two genotypes that were unique to the southernmost populations, and probably represent the most derived genotypes. We looked at genetic distances among populations in similar habitats, and found that populations were more closely related to their closest neighboring population than to a population in a similar habitat. We show that genotypic diversity within P. umbilicalis populations in the Gulf of Maine is relatively high and thus fits a model of high steady-state variation within asexual populations. C1 [Eriksen, Renee L.; Green, Lindsay A.; Klein, Anita S.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Eriksen, Renee L.] USDA ARS, 1636 East Alisal St, Salinas, CA 93905 USA. [Green, Lindsay A.] Univ Rhode Isl, Dept Biol Sci, Kingston, RI 02881 USA. RP Eriksen, RL (reprint author), Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA.; Eriksen, RL (reprint author), USDA ARS, 1636 East Alisal St, Salinas, CA 93905 USA. EM renee.eriksen@gmail.com FU New Hampshire Agricultural Experiment Station [2584]; USDA National Institute of Food and Agriculture Hatch Project [0222799] FX Samples at Schoodic Point, Acadia National Park were collected under permit number #ACAD-2012-SCI-0013 issued to Dr. Jessica Muhlin from April 16, 2012, to April 25, 2012. We thank Dr. Agnes Mols-Mortensen, Dr. Chris Benton, Yuanyu Cao, Greg Smith, Anna, and Sean and Darwin Harrison for help in collections. Dr. Chris Neefus provided culture room space, statistical advice, and valuable feedback. Dr. Andrew Price and Shay Polosky provided the Thermo Scientific NanoDrop 2000c and technical advice. Many thanks also to Jobriah Anderson at the Hubbard Center for Genomic Studies who conducted the genotyping. Partial funding was provided by the New Hampshire Agricultural Experiment Station. This is Scientific Contribution Number 2584. This work was supported by the USDA National Institute of Food and Agriculture Hatch Project 0222799. NR 53 TC 0 Z9 0 U1 7 U2 15 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0006-8055 EI 1437-4323 J9 BOT MAR JI Bot. Marina PD FEB PY 2016 VL 59 IS 1 BP 1 EP 12 DI 10.1515/bot-2015-0017 PG 12 WC Plant Sciences; Marine & Freshwater Biology SC Plant Sciences; Marine & Freshwater Biology GA DC9BG UT WOS:000369514700001 ER PT J AU Sigua, GC Novak, JM Watts, DW Szogi, AA Shumaker, PD AF Sigua, G. C. Novak, J. M. Watts, D. W. Szoegi, A. A. Shumaker, P. D. TI Impact of switchgrass biochars with supplemental nitrogen on carbon-nitrogen mineralization in highly weathered Coastal Plain Ultisols SO CHEMOSPHERE LA English DT Article DE Switchgrass biochars; Switchgrass residues; Carbon mineralization; Highly weathered soils; Coastal Plain Ultisols; Total inorganic nitrogen ID DREDGED MATERIALS; FORAGE PRODUCTIVITY; LAKE PANASOFFKEE; SOIL AMENDMENTS; AGRICULTURE; DECOMPOSITION; ENVIRONMENT; BIOSOLIDS; RESIDUES; DYNAMICS AB Although an increase in soil fertility is the most frequently reported benefit linked to adding biochar to soils, there is still a need to pursue additional research that will improve our understanding on the impact of soil fertility enhancement because the effect could vary greatly between switchgrass (Panicum virgatum, L) residues (USG) and switchgrass biochars (SG). We hypothesized that SG with supplemental nitrogen (N) would deliver more positive effects on carbon (C) and N mineralization than USG. The objective of this study was to evaluate the effects of USG and SG, with or without supplemental inorganic N fertilizer on C and N mineralization in highly weathered Coastal Plain Ultisols. The application rate for SG and USG based on a corn yield goal of 112 kg ha(-1) was 40 Mg ha(-1). Inorganic N was added at the rate of 100 kg N ha(-1), also based on a corn yield of 7.03 tons ha(-1). Experimental treatments were: control (CONT) soil; control with N (CONT N); switchgrass residues (USG); USG with N (USG + N); switchgrass biochars at 250 degrees C (250SG); SG at 250 degrees C with N (250SG N); SG at 500 degrees C (500SG); and SG at 500 degrees C with N (500SG N). Cumulative and net CO2-C evolution was increased by the additions of SG and USG especially when supplemented with N. Soils treated with 250SG (8.6 mg kg(-1)) had the least concentration of total inorganic nitrogen (TIN) while the greatest amount of TIN was observed from the CONT N (19.0 mg kg(-1)). Our results suggest that application of SG in the short term may cause N immobilization resulting in the reduction of TIN. Published by Elsevier Ltd. C1 [Sigua, G. C.; Novak, J. M.; Watts, D. W.; Szoegi, A. A.; Shumaker, P. D.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Sigua, GC (reprint author), USDA ARS CPSWPRC, 2611 West Lucas St, Florence, SC 29501 USA. EM gilbert.sigua@ars.usda.gov NR 47 TC 2 Z9 2 U1 6 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD FEB PY 2016 VL 145 BP 135 EP 141 DI 10.1016/j.chemosphere.2015.11.063 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC4MZ UT WOS:000369196300018 PM 26688249 ER PT J AU Stamatiadis, S Tsadilas, C Samaras, V Schepers, JS Eskridge, K AF Stamatiadis, S. Tsadilas, C. Samaras, V. Schepers, J. S. Eskridge, K. TI Nitrogen uptake and N-use efficiency of Mediterranean cotton under varied deficit irrigation and N fertilization SO EUROPEAN JOURNAL OF AGRONOMY LA English DT Article DE Soil nitrates; N losses; Rainfall; Plant fractions; Seeds; Roots ID WATER-USE EFFICIENCY; COMPONENTS; YIELD; ACCUMULATION; PHOSPHORUS; CORN AB Efficient N management is essential to optimize yields and reduce degradation of the environment, but requires knowledge of deficit irrigation effects on crop yields and crop N outputs. This study assessed the N content and N-use efficiency of cotton over the 2008 and 2009 growing seasons in a single field site of the Thessaly Plain (central Greece). The experiment consisted of nine treatments with three fertilizer rates (60,110 and 160 kg N ha(-1)) split into three irrigation levels (approx. 1.0, 0.7 and 0.4 of the amount applied by the producer). Reduced water supply induced a shift in the distribution of N within the plant with seeds becoming an N sink under conditions of water stress. Total crop N increased linearly with irrigation level and reached a maximum average of 261 and 192 kg N ha(-1) in 2008 and 2009, respectively. Fertilizer application did not trigger a crop N or yield response and indicated that N inputs were in excess of crop needs. Variation in weather patterns appeared to explain annual differences of nitrate-N in the top soil and N uptake by the crop. The index of lint production efficiency (iNUE) detected crop responses caused by irrigation and annual effects, but failed to account for excessive N inputs due to mineral fertilizer applications. A maximum average iNUE of 9.6 was obtained under deficit irrigation, whereas an iNUE of 8.1 was obtained under 40 cm irrigation when crop N uptake was not excessive (192 kg ha(-1) in 2009). In contrast, NUE, as an estimator of N recovery efficiency, identified excessive fertilizer inputs as N losses to the environment and indicated that 60 kg N ha(-1) was a rate of high N removal efficiency and long-term N balance. However, NUE failed to account for crop N responses to irrigation and weather/management patterns. In this case study, neither index was able to detect all the factors influencing the N mass balance and both were required in order to provide a comprehensive evaluation of the environmental performance of our cropping system. (C) 2015 Elsevier B.V. All rights reserved. C1 [Stamatiadis, S.] Goulandris Nat Hist Museum, Soil Ecol & Biotechnol Lab, 13 Levidou St, Kifisia 14562, Greece. [Tsadilas, C.; Samaras, V.] Natl Agr Res Fdn, Inst Soil Classificat & Mapping, 1 Theophrastou St, Larisa 41335, Greece. [Schepers, J. S.] Univ Nebraska, USDA ARS, 120 Keim Hall, Lincoln, NE 68583 USA. [Eskridge, K.] Univ Nebraska, Dept Stat, 340 Hardin Hall, Lincoln, NE 68583 USA. RP Stamatiadis, S (reprint author), Goulandris Nat Hist Museum, Soil Ecol & Biotechnol Lab, 13 Levidou St, Kifisia 14562, Greece. EM stam@gnhm.gr; tsadilas@lar.forthnet.gr; samarasv@otenet.gr; james.schepers@gmail.com; keskridg@unlserve.unl.edu FU European Union Framework Programme 6 FX This project was funded by the PLEIADes "Participatory multilevel EO-assisted tools for irrigation water management and agricultural decision-support" (European Union Framework Programme 6). We would like to thank Prof. N. Dalezios for his constant support, Dr. C. Christofides and E. Tsantila for performing the plant nitrogen analysis. Special thanks are also addressed to Fotis Ntafos, E. Mpakoyiannis, and V. Ntokouzis for their help in the establishment of the field experiments and to the staff of the soil analysis laboratory. NR 28 TC 1 Z9 1 U1 11 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1161-0301 EI 1873-7331 J9 EUR J AGRON JI Eur. J. Agron. PD FEB PY 2016 VL 73 BP 144 EP 151 DI 10.1016/j.eja.2015.11.013 PG 8 WC Agronomy SC Agriculture GA DC8HF UT WOS:000369459800015 ER PT J AU Siddiqua, TJ Ahmad, SM Ahsan, KB Rashid, M Roy, A Rahman, SM Shahab-Ferdows, S Hampel, D Ahmed, T Allen, LH Raqib, R AF Siddiqua, Towfida J. Ahmad, Shaikh M. Ahsan, Khalid B. Rashid, Mamunur Roy, Anjan Rahman, Syed M. Shahab-Ferdows, Setareh Hampel, Daniela Ahmed, Tahmeed Allen, Lindsay H. Raqib, Rubhana TI Vitamin B12 supplementation during pregnancy and postpartum improves B12 status of both mothers and infants but vaccine response in mothers only: a randomized clinical trial in Bangladesh SO EUROPEAN JOURNAL OF NUTRITION LA English DT Article DE Vitamin B12 supplementation; Methylmalonic acid; Breast milk B12; Vaccine response ID METHYLMALONIC ACID; SERUM VITAMIN-B-12; MICRONUTRIENT SUPPLEMENTATION; MATERNAL IMMUNIZATION; INSULIN-RESISTANCE; ELDERLY PATIENTS; BREAST-MILK; DEFICIENCY; WOMEN; FOLATE AB Poor vitamin B12 (B12) status is associated with adverse outcomes in pregnancy and infancy. Little is known about effects of B12 supplementation on immune function. The present study aimed to evaluate effects of pre- and postnatal B12 supplementation on biomarkers of B12 status and vaccine-specific responses in mothers and infants. In a blinded, placebo-controlled trial, Bangladeshi women (n = 68, age 18-35 years, hemoglobin < 110 g/L, 11-14 weeks pregnant) were randomized to receive 250 mu g/day B12 or a placebo throughout pregnancy and 3-month postpartum along with 60 mg iron + 400 mu g folate. Women were immunized with pandemic influenza A (H1N1) vaccine at 26- to 28-week gestation. Blood from mothers (baseline, 72-h post-delivery, 3-month postpartum), newborns and infants (3-month) was analyzed for hemoglobin, B12, methylmalonic acid (MMA), total homocysteine (tHcy), ferritin and serum transferrin receptor, C-reactive protein (CRP) and alpha-1-acid glycoprotein (AGP). Vitamin B12 was also assessed in breast milk. H1N1-specific antibodies were determined in plasma and colostrum/breast milk. At baseline, 26 % women were B12 deficient (< 150 pmol/L), 40 % had marginal status (150-220 pmol/L), 43 % had elevated MMA (> 271 nmol/L), and 31 % had elevated tHcy (> 10 mu mol/L). Supplementation increased B12 in plasma, colostrums and breast milk (p < 0.05) and lowered MMA in neonates, mothers and infants at 3 months (p < 0.05). B12 supplementation significantly increased H1N1-specific IgA responses in plasma and colostrums in mothers and reduced proportion of infants with elevated AGP and CRP compared with placebo. Supplementation with 250 mu g/day B12 during pregnancy and lactation substantially improved maternal, infant and breast milk B12 status. Maternal supplementation improved H1N1 vaccine-specific responses in mothers only and may alleviate inflammatory responses in infants. C1 [Siddiqua, Towfida J.; Ahmad, Shaikh M.; Ahsan, Khalid B.; Rashid, Mamunur; Roy, Anjan; Rahman, Syed M.; Ahmed, Tahmeed; Raqib, Rubhana] Int Ctr Diarrheal Dis Res Bangladesh, Nutr Biochem Lab, 68 Shaheed Tajuddin Ahmed Sarani, Dhaka 1212, Bangladesh. [Siddiqua, Towfida J.; Hampel, Daniela; Allen, Lindsay H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Shahab-Ferdows, Setareh; Hampel, Daniela; Allen, Lindsay H.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. RP Raqib, R (reprint author), Int Ctr Diarrheal Dis Res Bangladesh, Nutr Biochem Lab, 68 Shaheed Tajuddin Ahmed Sarani, Dhaka 1212, Bangladesh. EM rubhana@icddrb.org OI Raqib, Rubhana/0000-0002-8116-4511 FU Nestle Foundation [GR-00745]; Swedish International Development Cooperation Agency [GR-00599]; Bill & Melinda Gates Foundation [OPP1061055]; intramural USDA-ARS Project [5306-51000-003-00D] FX We wish to thank Dr. Md. Sirajul Islam and Dr. Chinmoy K. Das of MCHTI for the generous support and creation of a congenial atmosphere to conduct the study. We are indebted to the women who participated in this project, to the field staff, laboratory technicians, hospital nurses and staffs who made the study possible. We gratefully appreciate the help of Md. Shahidul Islam with statistical analysis. Dietary diversity questionnaire was obtained from Dr. Kuntal Kumar Saha (formerly at ICDDR, B; currently at International Food Policy Research Institute). The B12 and placebo pills were kind donation of Incepta Pharmaceuticals Ltd. The study was funded by the Nestle Foundation, (GR-00745), Swedish International Development Cooperation Agency (GR-00599), Bill & Melinda Gates Foundation (OPP1061055) and intramural USDA-ARS Project (5306-51000-003-00D). NR 55 TC 6 Z9 6 U1 6 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-6207 EI 1436-6215 J9 EUR J NUTR JI Eur. J. Nutr. PD FEB PY 2016 VL 55 IS 1 BP 281 EP 293 DI 10.1007/s00394-015-0845-x PG 13 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC6DJ UT WOS:000369309700027 PM 25648738 ER PT J AU Wang, LB Baldwin, EA Bai, JH AF Wang, Libin Baldwin, Elizabeth A. Bai, Jinhe TI Recent Advance in Aromatic Volatile Research in Tomato Fruit: The Metabolisms and Regulations SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Review DE Solanum lycopersicum; Volatile; Biosynthetic pathway; Ethylene; Genetics; Pre-harvest; Postharvest; Ripening ID METHYL JASMONATE PRETREATMENT; ACID HYDROPEROXIDE LYASE; ALLENE OXIDE SYNTHASE; FLAVOR VOLATILES; ETHYLENE BIOSYNTHESIS; CHILLING TEMPERATURE; GENE-EXPRESSION; RIPE TOMATOES; STRIGOLACTONE BIOSYNTHESIS; ALCOHOL-DEHYDROGENASE AB Aroma, an essential characteristic of tomato fruit, plays an important role in determining the perception and acceptability of tomato products by consumers. During tomato fruit ripening, associated with color changes from green to red involving the conversion of chloroplasts to chromoplasts are changes of aromatic volatile profiles. Although the biosynthetic pathways for some aromatic volatiles have been established in tomato fruit recently, our knowledge of regulatory mechanisms is still rudimentary. On the other hand, many internal and external factors modify volatile metabolism in tomato fruit. This review first summarizes the current knowledge of expression patterns and biosynthetic pathways of aromatic volatiles in tomato fruit along with the role of ethylene in their biosynthesis. The impact of internal and pre- and postharvest external factors on volatile composition is then discussed. This review will provide critical information for research on tomato aromatic volatiles and their manipulation. C1 [Wang, Libin; Baldwin, Elizabeth A.; Bai, Jinhe] ARS, USDA, Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. [Wang, Libin] Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. RP Bai, JH (reprint author), ARS, USDA, Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov FU China Scholarship Council [201306850049]; Postgraduate Program in Jiangsu Province [CXLX13 267] FX We thank the China Scholarship Council (201306850049) and Postgraduate Program in Jiangsu Province (CXLX13 267) for providing financial support for this study. NR 81 TC 1 Z9 1 U1 26 U2 70 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD FEB PY 2016 VL 9 IS 2 BP 203 EP 216 DI 10.1007/s11947-015-1638-1 PG 14 WC Food Science & Technology SC Food Science & Technology GA DC5OV UT WOS:000369271200001 ER PT J AU Bassar, RD Letcher, BH Nislow, KH Whiteley, AR AF Bassar, Ronald D. Letcher, Benjamin H. Nislow, Keith H. Whiteley, Andrew R. TI Changes in seasonal climate outpace compensatory density-dependence in eastern brook trout SO GLOBAL CHANGE BIOLOGY LA English DT Article DE climate change; density-dependence; integral projection models; metapopulations; salmonids; size-structure ID INTEGRAL PROJECTION MODELS; MATRIX POPULATION-MODELS; ATLANTIC SALMON; WATER TEMPERATURE; CUTTHROAT TROUT; FRESH-WATER; SURVIVAL; GROWTH; STREAM; LIFE AB Understanding how multiple extrinsic (density-independent) factors and intrinsic (density-dependent) mechanisms influence population dynamics has become increasingly urgent in the face of rapidly changing climates. It is particularly unclear how multiple extrinsic factors with contrasting effects among seasons are related to declines in population numbers and changes in mean body size and whether there is a strong role for density-dependence. The primary goal of this study was to identify the roles of seasonal variation in climate driven environmental direct effects (mean stream flow and temperature) vs. density-dependence on population size and mean body size in eastern brook trout (Salvelinus fontinalis). We use data from a 10-year capture-mark-recapture study of eastern brook trout in four streams in Western Massachusetts, USA to parameterize a discrete-time population projection model. The model integrates matrix modeling techniques used to characterize discrete population structures (age, habitat type, and season) with integral projection models (IPMs) that characterize demographic rates as continuous functions of organismal traits (in this case body size). Using both stochastic and deterministic analyses we show that decreases in population size are due to changes in stream flow and temperature and that these changes are larger than what can be compensated for through density-dependent responses. We also show that the declines are due mostly to increasing mean stream temperatures decreasing the survival of the youngest age class. In contrast, increases in mean body size over the same period are the result of indirect changes in density with a lesser direct role of climate-driven environmental change. C1 [Bassar, Ronald D.; Letcher, Benjamin H.] US Geol Survey, Leetown Sci Ctr, SO Conte Anadromous Fish Res Ctr, Turners Falls, MA 01376 USA. [Bassar, Ronald D.; Letcher, Benjamin H.; Nislow, Keith H.; Whiteley, Andrew R.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Nislow, Keith H.] Univ Massachusetts, USDA Forest Serv, No Res Stn, Amherst, MA 01003 USA. RP Bassar, RD (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA.; Bassar, RD (reprint author), Univ Oxford, Dept Zool, Oxford OX1 3PS, England. EM ronald.bassar@zoo.ox.ac.uk FU US Fish, Wildlife Service North Atlantic Conservation Cooperative; Massachusetts Agricultural Experiment Station [14] FX We thank the numerous students and interns who have helped to sample the West Brook study site. A special thanks to Todd Dubreuil, Matthew O'Donnell, and Jason Coombs for coordinating the logistics and field work at the West Brook study site. Thanks also to Krzysztof Sakrejda for maintaining the computer facilities at the University of Massachusetts Amherst which were used to analyze the model. Partial funding was provided by the US Fish, Wildlife Service North Atlantic Conservation Cooperative and from the Massachusetts Agricultural Experiment Station (project number MAS # 14). Any use of trade, firm, or product names is for descriptive purposes only and does imply endorsement by the U.S. Government. NR 53 TC 2 Z9 2 U1 11 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2016 VL 22 IS 2 BP 577 EP 593 DI 10.1111/gcb.13135 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DC3RC UT WOS:000369135400009 PM 26490737 ER PT J AU Voelker, SL Brooks, JR Meinzer, FC Anderson, R Bader, MKF Battipaglia, G Becklin, KM Beerling, D Bert, D Betancourt, JL Dawson, TE Domec, JC Guyette, RP Korner, C Leavitt, SW Linder, S Marshall, JD Mildner, M Ogee, J Panyushkina, I Plumpton, HJ Pregitzer, KS Saurer, M Smith, AR Siegwolf, RTW Stambaugh, MC Talhelm, AF Tardif, JC Van de Water, PK Ward, JK Wingate, L AF Voelker, Steven L. Brooks, J. Renee Meinzer, Frederick C. Anderson, Rebecca Bader, Martin K. -F. Battipaglia, Giovanna Becklin, Katie M. Beerling, David Bert, Didier Betancourt, Julio L. Dawson, Todd E. Domec, Jean-Christophe Guyette, Richard P. Koerner, Christian Leavitt, Steven W. Linder, Sune Marshall, John D. Mildner, Manuel Ogee, Jerome Panyushkina, Irina Plumpton, Heather J. Pregitzer, Kurt S. Saurer, Matthias Smith, Andrew R. Siegwolf, Rolf T. W. Stambaugh, Michael C. Talhelm, Alan F. Tardif, Jacques C. Van de Water, Peter K. Ward, Joy K. Wingate, Lisa TI A dynamic leaf gas-exchange strategy is conserved in woody plants under changing ambient CO2: evidence from carbon isotope discrimination in paleo and CO2 enrichment studies SO GLOBAL CHANGE BIOLOGY LA English DT Article DE angiosperm; carbon dioxide; free-air CO2 enrichment; gymnosperm; optimal stomatal behavior; photosynthesis; stomatal conductance; water use efficiency ID WATER-USE EFFICIENCY; VAPOR-PRESSURE DEFICIT; RING STABLE-ISOTOPES; LAST GLACIAL MAXIMUM; ANTARCTIC ICE CORE; FOREST FACE SITES; ELEVATED CO2; TREE-RINGS; ATMOSPHERIC CO2; MESOPHYLL CONDUCTANCE AB Rising atmospheric [CO2], c(a), is expected to affect stomatal regulation of leaf gas-exchange of woody plants, thus influencing energy fluxes as well as carbon (C), water, and nutrient cycling of forests. Researchers have proposed various strategies for stomatal regulation of leaf gas-exchange that include maintaining a constant leaf internal [CO2], c(i), a constant drawdown in CO2 (c(a)-c(i)), and a constant c(i)/c(a). These strategies can result in drastically different consequences for leaf gas-exchange. The accuracy of Earth systems models depends in part on assumptions about generalizable patterns in leaf gas-exchange responses to varying c(a). The concept of optimal stomatal behavior, exemplified by woody plants shifting along a continuum of these strategies, provides a unifying framework for understanding leaf gas-exchange responses to c(a). To assess leaf gas-exchange regulation strategies, we analyzed patterns in c(i) inferred from studies reporting C stable isotope ratios (C-13) or photosynthetic discrimination () in woody angiosperms and gymnosperms that grew across a range of c(a) spanning at least 100ppm. Our results suggest that much of the c(a)-induced changes in c(i)/c(a) occurred across c(a) spanning 200 to 400ppm. These patterns imply that c(a)-c(i) will eventually approach a constant level at high c(a) because assimilation rates will reach a maximum and stomatal conductance of each species should be constrained to some minimum level. These analyses are not consistent with canalization toward any single strategy, particularly maintaining a constant c(i). Rather, the results are consistent with the existence of a broadly conserved pattern of stomatal optimization in woody angiosperms and gymnosperms. This results in trees being profligate water users at low c(a), when additional water loss is small for each unit of C gain, and increasingly water-conservative at high c(a), when photosystems are saturated and water loss is large for each unit C gain. C1 [Voelker, Steven L.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Brooks, J. Renee] US EPA, Western Ecol Div, NHEERL, 200 SW 35Th St, Corvallis, OR 97333 USA. [Meinzer, Frederick C.] USDA, Forest Serv, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Anderson, Rebecca] Univ Calif Santa Cruz, Jack Baskin Engn, Santa Cruz, CA 95604 USA. [Bader, Martin K. -F.] New Zealand Forest Res Inst SCION, Te Papa Tipu Innovat Pk,20 Sala St, Rotorua 3046, New Zealand. [Battipaglia, Giovanna] Univ Naples 2, Dept Environm Biol & Pharmaceut Sci & Technol DiS, I-81100 Caserta, Italy. [Battipaglia, Giovanna] Univ Montpellier 2, Ctr Bioarchaeol & Ecol, Ecole Prat Hautes Etud, Inst Bot, F-34090 Montpellier, France. [Becklin, Katie M.; Ward, Joy K.] Univ Kansas, Dept Ecol & Evolutionary Biol, 1200 Sunnyside Ave, Lawrence, KS 66045 USA. [Beerling, David] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England. [Bert, Didier] INRA, BIOGECO UMR1202, F-33610 Cestas, France. [Bert, Didier] Univ Bordeaux, UMR BIOGECO 1202, F-33615 Pessac, France. [Betancourt, Julio L.] US Geol Survey, Natl Res Program, Water Mission Area, Mail Stop 430,12201 Sunrise Valley Dr, Reston, VA 20192 USA. [Dawson, Todd E.] Univ Calif Berkeley, Dept Integrat Biol, 1105 Valley Life Sci Bldg 3140, Berkeley, CA 94720 USA. [Domec, Jean-Christophe; Ogee, Jerome; Wingate, Lisa] INRA, Bordeaux Sci Agro, UMR ISPA 1391, F-33175 Gradignan, France. [Domec, Jean-Christophe] Duke Univ, Nicholas Sch Environm, Box 90328, Durham, NC 27708 USA. [Guyette, Richard P.; Stambaugh, Michael C.] Univ Missouri, Dept Forestry, 203 ABNR Bldg, Columbia, MO 65211 USA. [Koerner, Christian; Mildner, Manuel] Univ Basel, Inst Bot, Schonbeinstr 6, CH-4056 Basel, Switzerland. [Leavitt, Steven W.; Plumpton, Heather J.; Wingate, Lisa] INRA, ISPA UMR1391, F-33140 Villenave Dornon, France. [Linder, Sune; Panyushkina, Irina] Univ Arizona, Tree Ring Res Lab, 1215 E Lowell St, Tucson, AZ 85721 USA. [Marshall, John D.] Swedish Univ Agr Sci, Southern Swedish Forest Res Ctr, POB 49, SE-23053 Alnarp, Sweden. [Marshall, John D.] Swedish Univ Agr Sci, Dept Forest Ecol & Management, SE-90183 Umea, Sweden. [Pregitzer, Kurt S.; Talhelm, Alan F.] Univ Idaho, Dept Forest Rangeland & Fire Sci, 875 Perimeter Dr, Moscow, ID 83844 USA. [Saurer, Matthias; Siegwolf, Rolf T. W.] Paul Scherrer Inst, CH-5323 Villigen, Switzerland. [Smith, Andrew R.] Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2UW, Gwynedd, Wales. [Tardif, Jacques C.] Univ Winnipeg, C FIR, 515 Ave Portage, Winnipeg, MB R3B 2E9, Canada. [Van de Water, Peter K.] Calif State Univ Fresno, Dept Earth & Environm Sci, 2576 E San Ramon Ave,Mail Stop ST-24, Fresno, CA 93740 USA. RP Voelker, SL (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM dr.s.voelker@gmail.com RI Smith, Andy/A-7512-2011; Siegwolf, Rolf/D-4121-2009; Ogee, Jerome/C-7185-2013; Wingate, Lisa/G-5575-2015 OI Smith, Andy/0000-0001-8580-278X; Siegwolf, Rolf/0000-0002-0249-0651; Wingate, Lisa/0000-0003-1921-1556 FU National Science Foundation [DEB-0743882, AGS-1003601, NSF-EAR-1344703]; INRA EFPA Projet Innovant grant; French Research Agency [MACACC ANR-13-AGRO-0005, MARIS ANR-14-CE03-0007] FX We thank Dr. Jeff Warren, Dr. Jon Keeley, Dr. Jim Ehleringer and an anonymous reviewer for providing comments that greatly improved the manuscript and the many contributors who helped produce the data sets analyzed here. Research by S. Voelker was supported by the National Science Foundation grants DEB-0743882 and AGS-1003601. JCD, LW, JO and HJP acknowledge financial support from the INRA EFPA Projet Innovant (2012) grant awarded to LW and from the French Research Agency (projects MACACC ANR-13-AGRO-0005 and MARIS ANR-14-CE03-0007) and the National Science Foundation (NSF-EAR-1344703). We would also like to thank James Rolfe at the Godwin Laboratory at the University of Cambridge for his responsive analysis of the DUKE Face tree ring material. This manuscript has been subjected to the Environmental Protection Agency's peer and administrative review, and it has been approved for publication as an EPA document. It also underwent internal review and received formal approval by the U.S. Geological Survey. Mention of trade names or commercial products does not constitute endorsement or a recommendation for use. NR 106 TC 5 Z9 5 U1 18 U2 74 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2016 VL 22 IS 2 BP 889 EP 902 DI 10.1111/gcb.13102 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DC3RC UT WOS:000369135400032 PM 26391334 ER PT J AU Franca, TSFA Franca, FJN Arango, RA Woodward, BM Arantes, MDC AF Franca, Tamara Suely Filgueira Amorim Franca, Frederico Jose Nistal Arango, Rachel A. Woodward, Bessie M. Chaves Arantes, Marina Donaria TI Natural resistance of plantation grown African mahogany (Khaya ivorensis and Khaya senegalensis) from Brazil to wood-rot fungi and subterranean termites SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Khaya ivorensis; Khaya senegalensis; Natural durability; Fungal decay; Termite damage ID TROPICAL HARDWOODS; DURABILITY AB African mahogany (Khaya spp.) has attracted the interest of the timber market in Brazil because of the quality of the wood and the similarity to the highly demanded, Brazilian mahogany (S. macrophylla King). The goal of this study was to examine natural resistance of plantation-grown African mahogany (Khaya spp.) to decay fungi and termite feeding, in order to better evaluate the potential use of this material as a suitable replacement for Brazilian mahogany wood. Heartwood and sapwood of two African mahogany species, Khaya ivorensis and Khaya senegalensis, were evaluated for resistance to decay by five wood-rot fungi as well as to feeding by subterranean termites in laboratory tests. In addition, density values were evaluated and examined for correlation to the observed natural durability properties. Overall, results showed heartwood of both species to be more resistant than sapwood to all fungi tested. K. senegalensis sapwood showed the lowest resistance to decay fungi, while K. senegalensis heartwood had the highest resistance to both brown-and white-rot fungi as well as to the dry-rot fungus tested. Both wood species showed some resistance to feeding by subterranean termites, with significantly higher resistance in heartwood compared to sapwood. In fungal and termite tests, durability was not found to be correlated to density values for either Khaya spp. tested. Results from this study suggest plantation-grown African mahogany exhibits similar natural durability properties as Brazilian mahogany, supporting the potential for its use as a suitable substitute to better meet the demands of the wood products industry. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Franca, Tamara Suely Filgueira Amorim; Franca, Frederico Jose Nistal] Mississippi State Univ, Dept Sustainable Bioprod, 201 Locksley Way, Starkville, MS 39759 USA. [Arango, Rachel A.; Woodward, Bessie M.] USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Chaves Arantes, Marina Donaria] Univ Fed Espirito Santo, Dept Forest & Wood Sci, 316 Governador Lindemberg Ave, BR-29550000 Jeronimo Monteiro, ES, Brazil. RP Franca, TSFA (reprint author), Mississippi State Univ, Dept Sustainable Bioprod, 201 Locksley Way, Starkville, MS 39759 USA. EM tamarasfamorim@gmail.com NR 21 TC 1 Z9 1 U1 3 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD FEB PY 2016 VL 107 BP 88 EP 91 DI 10.1016/j.ibiod.2015.11.009 PG 4 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA DC4QZ UT WOS:000369206700012 ER PT J AU Westbrook, JK Nagoshi, RN Meagher, RL Fleischer, SJ Jairam, S AF Westbrook, J. K. Nagoshi, R. N. Meagher, R. L. Fleischer, S. J. Jairam, S. TI Modeling seasonal migration of fall armyworm moths SO INTERNATIONAL JOURNAL OF BIOMETEOROLOGY LA English DT Article DE Dispersal; HYSPLIT; Insect; Lepidoptera; Spodoptera frugiperda; Corn-strain ID CORN-EARWORM LEPIDOPTERA; SPODOPTERA-FRUGIPERDA LEPIDOPTERA; LONG-DISTANCE MIGRATION; NOCTUIDAE HOST STRAINS; POPULATION-DYNAMICS; UNITED-STATES; TEXAS; FLORIDA; HELICOVERPA; INSECT AB Fall armyworm, Spodoptera frugiperda (J.E. Smith), is a highly mobile insect pest of a wide range of host crops. However, this pest of tropical origin cannot survive extended periods of freezing temperature but must migrate northward each spring if it is to re-infest cropping areas in temperate regions. The northward limit of the winter-breeding region for North America extends to southern regions of Texas and Florida, but infestations are regularly reported as far north as Qu,bec and Ontario provinces in Canada by the end of summer. Recent genetic analyses have characterized migratory pathways from these winter-breeding regions, but knowledge is lacking on the atmosphere's role in influencing the timing, distance, and direction of migratory flights. The Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model was used to simulate migratory flight of fall armyworm moths from distinct winter-breeding source areas. Model simulations identified regions of dominant immigration from the Florida and Texas source areas and overlapping immigrant populations in the Alabama-Georgia and Pennsylvania-Mid-Atlantic regions. This simulated migratory pattern corroborates a previous migratory map based on the distribution of fall armyworm haplotype profiles. We found a significant regression between the simulated first week of moth immigration and first week of moth capture (for locations which captured a parts per thousand yen10 moths), which on average indicated that the model simulated first immigration 2 weeks before first captures in pheromone traps. The results contribute to knowledge of fall armyworm population ecology on a continental scale and will aid in the prediction and interpretation of inter-annual variability of insect migration patterns including those in response to climatic change and adoption rates of transgenic cultivars. C1 [Westbrook, J. K.; Jairam, S.] Texas A&M Univ, USDA ARS, College Stn, TX 77843 USA. [Nagoshi, R. N.; Meagher, R. L.] Univ Florida, USDA ARS, Gainesville, FL 32611 USA. [Fleischer, S. J.] Penn State Univ, State Coll, PA USA. [Westbrook, J. K.] ARS, Insect Control & Cotton Dis Res Unit, USDA, SPARC, 2771 F&B Rd, College Stn, TX 77845 USA. RP Westbrook, JK (reprint author), Texas A&M Univ, USDA ARS, College Stn, TX 77843 USA.; Westbrook, JK (reprint author), ARS, Insect Control & Cotton Dis Res Unit, USDA, SPARC, 2771 F&B Rd, College Stn, TX 77845 USA. EM john.westbrook@ars.usda.gov FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-67003-30209] FX T. M. O'Neil, T. Sappington, B. R. Leonard, K. Estes, J. Obermeyer, G. Lorenz, B. McCornack, K. Tindall, A. Michel, T. Baute, P. Porter, J. S. Armstrong, E. Burkness, D. Cook, F. Musser, J. Knodel, J. Bradshaw, R. Wright, T. Hunt, T. Royer, R. Parker, A. Knutson, H. Stevens, E. Cullen, F. Peairs, J. Whalen, S. Menasha, J. Ingerson-Mahar, K. Holmstrom, T. Kuhar, A. Herbert, G. Dively, A. Muza, J. Timer, R. Pollack, T. Ford, R. Troyer, K. Watrous, A. Bachmann, C. Sidhu, C. Loveland, T. Abbey, T. Elkner, E. Swackhammer, and T. Bailey assisted in trapping moths and reporting weekly moth capture data. This work was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Agreement No. 2011-67003-30209. USDC-NOAA provided access to HYSPLIT PC version 4.9 atmospheric transport modeling software. Mention of trade names or commercial products in this article is solely for the purposes of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 59 TC 4 Z9 4 U1 10 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0020-7128 EI 1432-1254 J9 INT J BIOMETEOROL JI Int. J. Biometeorol. PD FEB PY 2016 VL 60 IS 2 BP 255 EP 267 DI 10.1007/s00484-015-1022-x PG 13 WC Biophysics; Environmental Sciences; Meteorology & Atmospheric Sciences; Physiology SC Biophysics; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Physiology GA DC6KR UT WOS:000369329300007 PM 26045330 ER PT J AU Verhoef, PA Constantinides, MG McDonald, BD Urban, JF Sperling, AI Bendelac, A AF Verhoef, Philip A. Constantinides, Michael G. McDonald, Benjamin D. Urban, Joseph F., Jr. Sperling, Anne I. Bendelac, Albert TI Intrinsic functional defects of type 2 innate lymphoid cells impair innate allergic inflammation in promyelocytic leukemia zinc finger (PLZF)-deficient mice SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article DE Allergic mechanisms; innate lymphoid cells; mouse models ID NATURAL HELPER-CELLS; LUNG INFLAMMATION; T-CELLS; AIRWAY INFLAMMATION; IMMUNE-RESPONSE; NKT CELLS; ASTHMA; HOMEOSTASIS; LINEAGE; HYPERREACTIVITY AB Background: The transcription factor promyelocytic leukemia zinc finger (PLZF) is transiently expressed during development of type 2 innate lymphoid cells (ILC2s) but is not present at the mature stage. We hypothesized that PLZF-deficient ILC2s have functional defects in the innate allergic response and represent a tool for studying innate immunity in a mouse with a functional adaptive immune response. Objective: We determined the consequences of PLZF deficiency on ILC2 function in response to innate and adaptive immune stimuli by using PLZF(-/-) mice and mixed wild-type:PLZF(-/-) bone marrow chimeras. Methods: PLZF(-/-) mice, wild-type littermates, or mixed bone marrow chimeras were treated with the protease allergen papain or the cytokines IL-25 and IL-33 or infected with the helminth Nippostrongylus brasiliensis to induce innate type 2 allergic responses. Mice were sensitized with intraperitoneal ovalbumin-alum, followed by intranasal challenge with ovalbumin alone, to induce adaptive T(H)2 responses. Lungs were analyzed for immune cell subsets, and alveolar lavage fluid was analyzed for ILC2-derived cytokines. In addition, ILC2s were stimulated ex vivo for their capacity to release type 2 cytokines. Results: PLZF-deficient lung ILC2s exhibit a cell-intrinsic defect in the secretion of IL-5 and IL-13 in response to innate stimuli, resulting in defective recruitment of eosinophils and goblet cell hyperplasia. In contrast, the adaptive allergic inflammatory response to ovalbumin and alum was unimpaired. Conclusions: PLZF expression at the innate lymphoid cell precursor stage has a long-range effect on the functional properties of mature ILC2s and highlights the importance of these cells for innate allergic responses in otherwise immunocompetent mice. C1 [Verhoef, Philip A.; Constantinides, Michael G.; McDonald, Benjamin D.; Sperling, Anne I.; Bendelac, Albert] Univ Chicago, Comm Immunol, Chicago, IL 60637 USA. [Verhoef, Philip A.; Sperling, Anne I.] Univ Chicago, Dept Med, Sect Pulm & Crit Care, Chicago, IL 60637 USA. [Verhoef, Philip A.] Univ Chicago, Dept Pediat, Sect Pediat Crit Care, Chicago, IL 60637 USA. [Bendelac, Albert] Univ Chicago, Dept Pathol, Chicago, IL 60637 USA. [Urban, Joseph F., Jr.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. RP Bendelac, A (reprint author), Univ Chicago, Dept Pathol, Comm Immunol, 929 57th St,Gordon Ctr Integrat Sci W506, Chicago, IL 60637 USA. EM abendela@bsd.uchicago.edu OI Constantinides, Michael/0000-0003-4770-7751 FU National Institutes of Health [T32 HL007605, R01HL118092, R01AI038339, AI108643]; University of Chicago Digestive Research Core Center Grant [P30DK42086] FX Supported by National Institutes of Health grants T32 HL007605 (to P.A.V.) and R01HL118092, R01AI038339, and AI108643 (A.B.) and the University of Chicago Digestive Research Core Center Grant P30DK42086. NR 24 TC 2 Z9 2 U1 1 U2 4 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 BP 591 EP + DI 10.1016/j.jaci.2015.07.050 PG 11 WC Allergy; Immunology SC Allergy; Immunology GA DC5BN UT WOS:000369235500030 PM 26602165 ER PT J AU Yang, Y Endreny, TA Nowak, DJ AF Yang, Yang Endreny, Theodore A. Nowak, David J. TI Application of advection-diffusion routing model to flood wave propagation: A case study on Big Piney River, Missouri USA SO JOURNAL OF EARTH SCIENCE LA English DT Article DE advection-diffusion equation; hydrograph; flood wave propagation; recession limb ID HYDROGRAPH; TRANSPORT AB Flood wave propagation modeling is of critical importance to advancing water resources management and protecting human life and property. In this study, we investigated how the advection-diffusion routing model performed in flood wave propagation on a 16 km long downstream section of the Big Piney River, MO. Model performance was based on gaging station data at the upstream and downstream cross sections. We demonstrated with advection-diffusion theory that for small differences in watershed drainage area between the two river cross sections, inflow along the reach mainly contributes to the downstream hydrograph's rising limb and not to the falling limb. The downstream hydrograph's falling limb is primarily determined by the propagated flood wave originating at the upstream cross section. This research suggests the parameter for the advectiondiffusion routing model can be calibrated by fitting the hydrograph falling limb. Application of the advection diffusion model to the flood wave of January 29, 2013 supports our theoretical finding that the propagated flood wave determines the downstream cross section falling limb, and the model has good performance in our test examples. C1 [Yang, Yang; Nowak, David J.] US Forest Serv, USDA, No Res Stn, Syracuse, NY 13210 USA. [Yang, Yang] Davey Inst, Syracuse, NY 13210 USA. [Endreny, Theodore A.] SUNY ESF, Environm Resource Engn, Syracuse, NY 13210 USA. RP Yang, Y (reprint author), US Forest Serv, USDA, No Res Stn, Syracuse, NY 13210 USA. EM yyang31@syr.edu FU USDA Forest Service Northern Research Station iTree Spatial Simulation [PL-5937]; National Urban and Community Forest Advisory Council iTree Tool [11-DG-11132544-340] FX This research was supported by funding from the USDA Forest Service Northern Research Station iTree Spatial Simulation (No. PL-5937), and the National Urban and Community Forest Advisory Council iTree Tool (No. 11-DG-11132544-340). The SUNY ESF Department of Environmental Resources Engineering provided computing facilities and logistical support. The final publication is available at Springer via http://dx.doi.org/10.1007/s12583-016-0626-3. NR 28 TC 1 Z9 1 U1 2 U2 11 PU CHINA UNIV GEOSCIENCES PI BEIJING PA 29 XUEYUAN RD, BEIJING, 100083, PEOPLES R CHINA SN 1674-487X EI 1867-111X J9 J EARTH SCI-CHINA JI J. Earth Sci. PD FEB PY 2016 VL 27 IS 1 BP 9 EP 14 DI 10.1007/s12583-016-0626-3 PG 6 WC Geosciences, Multidisciplinary SC Geology GA DC5CY UT WOS:000369239800002 ER PT J AU Cox, NA Richardson, LJ Cosby, DE Berrang, ME Harrison, MA AF Cox, N. A. Richardson, L. J. Cosby, D. E. Berrang, M. E. Harrison, M. A. TI Recovery of Campylobacter from External and Internal Spleen Samples from Baby Broiler Chicks Following Various Routes of Inoculation SO JOURNAL OF FOOD SAFETY LA English DT Article ID COMMERCIAL BROILERS; BREEDER HENS; SALMONELLA SEROVARS; JEJUNI AB Campylobacter have been found in lymphoid tissue of poultry, but the location in these tissues has not been determined. The objective of this study was to determine if Campylobacter are on the outside or on the inside of the spleen. For external (ES) spleen sampling 2 days post-inoculation, each spleen was rinsed with 3mL of Bolton's enrichment broth (BEB). For internal (IS) spleen sampling, each rinsed spleen was submerged into 70% ethanol for 10s, removed and submerged into 0.85% saline. The spleen was then placed into a sterile bag, macerated with 3mL of BEB and stomached for 30s before incubation. All samples and the correlating ceca were sampled for Campylobacter jejuni. Overall in four different experiments with baby chicks inoculated by oral, ocular or cloacal routes, Campylobacter was recovered from 75% (82/109) of the ES, 71% (77/109) of the IS and from 100% (109/109) of the ceca. Ethanol and saline samples were all negative for Campylobacter, suggesting that ethanol immersion is adequate to kill C.jejuni on the external surface. With Campylobacter in the internal tissue of the spleen, the organism appears to be systemic. Further research will determine the colonization mechanisms required for this bacterium in these different tissues. Practical ApplicationsIt is very difficult to sample the free flowing blood of a living chicken for bacteria without introducing contamination from the skin. This paper describes an effective method of sampling the internal portion of the spleen without introducing external contamination. By doing so, this indicates that the bacteria were systemic and will travel with the blood to all of the internal organs and tissues throughout the bird's body. Therefore when the bacteria are not isolated from certain tissues, it means that reservoirs were either not established or did not persist in that particular tissue even though the bacteria did flow through with the blood. Unless all of the potential reservoirs and sources of bacteria such as Campylobacter and Salmonella are identified and fully understood, successful intervention strategies may not be possible. It would be important to know if reservoirs of these bacteria present in internal organs and tissues can periodically seed the intestinal and/or reproductive tracts of adult breeder birds, thus providing a source of contamination to the offspring. C1 [Cox, N. A.; Richardson, L. J.; Cosby, D. E.; Berrang, M. E.] ARS, USDA, Russell Res Ctr, Athens, GA USA. [Richardson, L. J.] Coca Cola Co, Atlanta, GA USA. [Harrison, M. A.] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. RP Cox, NA (reprint author), ARS, USDA, Russell Res Ctr, Athens, GA USA. EM nelson.cox@ars.usda.gov NR 12 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0149-6085 EI 1745-4565 J9 J FOOD SAFETY JI J. Food Saf. PD FEB PY 2016 VL 36 IS 1 BP 132 EP 135 DI 10.1111/jfs.12220 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DC6OS UT WOS:000369340100014 ER PT J AU Hashimoto, N Blumberg, JB Chen, CYO AF Hashimoto, Naoto Blumberg, Jeffrey B. Chen, C. -Y. Oliver TI Hyperglycemia and Anthocyanin Inhibit Quercetin Metabolism in HepG2 Cells SO JOURNAL OF MEDICINAL FOOD LA English DT Article DE cyanidin-3-glucoside; human hepatoma; hyperglycemic milieu; quercetin metabolism ID P38 MAPK; POLYPHENOLS; ACTIVATION; EXPRESSION; ENZYMES; LIVER; GLUCURONIDATION; INFLAMMATION; FLAVONOIDS; PATHWAYS AB A high glucose (Glu) milieu promotes generation of reactive oxygen species, which may not only cause cellular damage, but also modulate phase II enzymes that are responsible for the metabolism of flavonoids. Thus, we examined the effect of a high Glu milieu on quercetin (Q) metabolism in HepG2 cells. HepG2 cells were grown for 3 days in Glu ranging from 5.5 to 50mmol/L and/or cyanidin-3-glucoside (C3G) ranging from 0 to 25mol/L. Subsequently, the capacity of HepG2 cells to metabolize Q was assessed for up to 16h. Q metabolites were analyzed by high-performance liquid chromatography. Four major Q metabolites were observed in the culture medium and inside the HepG2 cells. Three of these metabolites appear to be sulfated forms of Q or methylated Q, and one was a methylated Q. These metabolites and Q itself were reduced or tended to be reduced in cells grown in a high Glu compared to a normal Glu medium. Addition of C3G or superoxide dismutase plus catalase did not prevent or enhance reduction of Q metabolites. In vitro, a hyperglycemic milieu decreases the production of the principal Q metabolites in HepG2 cells, mediated through mechanisms independent of oxidative stress. C1 [Hashimoto, Naoto; Blumberg, Jeffrey B.; Chen, C. -Y. Oliver] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Hashimoto, Naoto] Natl Agr Res Ctr Kyushu Okinawa Reg, Koshi Headquarters, Koshi, Kumamoto, Japan. [Hashimoto, Naoto] Natl Agr Res Ctr Hokkaido Reg, Memuro Res Stn, Kasai, Hokkaido, Japan. RP Chen, CYO (reprint author), Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM oliver.chen@tufts.edu FU Ministry of Agriculture, Forestry, and Fisheries in Japan; U.S. Department of Agriculture (USDA)/Agricultural Research Service [1950-51000-087] FX Supported by the Ministry of Agriculture, Forestry, and Fisheries in Japan and the U.S. Department of Agriculture (USDA)/Agricultural Research Service under Cooperative Agreement No. 1950-51000-087. The contents of this publication do not necessarily reflect the views or policies of the USDA nor does mention of trade names, commercial products, or organizations that imply endorsement by the U.S. government. NR 35 TC 0 Z9 0 U1 0 U2 9 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1096-620X EI 1557-7600 J9 J MED FOOD JI J. Med. Food PD FEB 1 PY 2016 VL 19 IS 2 BP 141 EP 147 DI 10.1089/jmf.2015.0089 PG 7 WC Chemistry, Medicinal; Food Science & Technology; Nutrition & Dietetics SC Pharmacology & Pharmacy; Food Science & Technology; Nutrition & Dietetics GA DC8YA UT WOS:000369505000005 PM 26692239 ER PT J AU Dwarkanath, P Hsu, JW Tang, GJ Anand, P Thomas, T Thomas, A Sheela, CN Kurpad, AV Jahoor, F AF Dwarkanath, Pratibha Hsu, Jean W. Tang, Grace J. Anand, Pauline Thomas, Tinku Thomas, Annamma Sheela, C. N. Kurpad, Anura V. Jahoor, Farook TI Energy and Protein Supplementation Does Not Affect Protein and Amino Acid Kinetics or Pregnancy Outcomes in Underweight Indian Women SO JOURNAL OF NUTRITION LA English DT Article DE leucine; glycine; serine; urea; birth weight; maternal weight gain; low birth weight ID BODY-MASS INDEXES; GLUCOSE KINETICS; METABOLISM; BIRTH; ADOLESCENT; LEUCINE AB Background: In India, the prevalence of low birth weight is high in women with a low body mass index (BMI), suggesting that underweight women are not capable of providing adequate energy and protein for fetal growth. Furthermore, as pregnancy progresses, there is increased need to provide methyl groups for methylation reactions associated with the synthesis of new proteins and, unlike normal-BMI American women, low-BMI Indian women are unable to increase methionine transmethylation and remethylation rates as pregnancy progresses from trimester 1 to 3. This also negatively influences birth weight. Objective: The aim was to determine the effect of dietary supplementation with energy and protein from 12 +/- 1 wk of gestation to time of delivery compared with no supplement on pregnancy outcomes, protein kinetics, and the fluxes of the methyl group donors serene and glycine. Methods: Protein kinetics and serine and glycine fluxes were measured by using standard stable isotope tracer methods in the fasting and postprandial states in 24 pregnant women aged 22.9 +/- 0.7 y with low BMIs [BMI (in kg/m(2)) <= 18.51 at 12 +/- 1 wk (trimester 1) and 30 +/- 1 wk (trimester 3) of gestation. After the first measurement, subjects were randomly assigned to either receive the supplement (300 kcal/d, 15 g protein/d) or no supplement. Results: Supplementation had no significant effect on any variable of pregnancy outcome, and except for fasting state decreases in leucine flux (125 +/- 7.14 compared with 113 +/- 5.06 mu mol . kg(-1) . h(-1); P = 0.04) and nonoxidative disposal (110 +/- 6.97 compared with 101 +/- 3.69 mu mol . kg(-1) . h(-1); P=0.02) from trimesters 1 to 3, it had no effect on any other leucine kinetic variable or urea, glycine, and serine fluxes. Conclusion: We conclude that in Indian women with a low BMI, supplementation with energy and protein from week 12 of pregnancy to time of delivery does not improve pregnancy outcome, whole-body protein kinetics, or serine and glycine fluxes. C1 [Dwarkanath, Pratibha; Anand, Pauline; Thomas, Tinku; Kurpad, Anura V.] St Johns Res Inst, Div Nutr, Bangalore, Karnataka, India. [Hsu, Jean W.; Tang, Grace J.; Jahoor, Farook] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. [Thomas, Annamma; Sheela, C. N.] St Johns Med Coll Hosp, Dept Obstet & Gynecol, Bangalore, Karnataka, India. RP Jahoor, F (reprint author), USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. EM fjahoor@bcm.edu FU USDA, Agricultural Research Service [58-6250-6001] FX Supported by federal funds from the USDA, Agricultural Research Service, under Cooperative Agreement 58-6250-6001. NR 23 TC 1 Z9 1 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2016 VL 146 IS 2 BP 218 EP 226 DI 10.3945/jn.115.218776 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC9RD UT WOS:000369557800006 PM 26764317 ER PT J AU Moran, NE Novotny, JA Cichon, MJ Riedl, KM Rogers, RB Grainger, EM Schwartz, SJ Erdman, JW Clinton, SK AF Moran, Nancy E. Novotny, Janet A. Cichon, Morgan J. Riedl, Kenneth M. Rogers, Randy B. Grainger, Elizabeth M. Schwartz, Steven J. Erdman, John W., Jr. Clinton, Steven K. TI Absorption and Distribution Kinetics of the C-13-Labeled Tomato Carotenoid Phytoene in Healthy Adults SO JOURNAL OF NUTRITION LA English DT Article DE phytoene; tomato; compartmental modeling; kinetics; carotenoid ID PROSTATE-CANCER; BETA-CAROTENE; SERUM LYCOPENE; CELL-CULTURE; IN-VITRO; PHYTOFLUENE; RISK; METABOLISM; MICE; MEN AB Background: Phytoene is a tomato carotenoid that may contribute to the apparent health benefits of tomato consumption. Although phytoene is a less prominent tomato carotenoid than lycopene, it is a major carotenoid in various human tissues. Phytoene distribution to plasma lipoproteins and tissues differs from lycopene, suggesting the kinetics of phytoene and lycopene differ. Objective: The objective of this study was to characterize the kinetic parameters of phytoene absorption, distribution, and excretion in adults, to better understand why biodistribution of phytoene differs from lycopene. Methods: Four adults (2 males, 2 females) maintained a controlled phytoene diet (1-5 mg/d) for 42 d. On day 14, each consumed 3.2 mg C-13-phytoene, produced using tomato cell suspension culture technology. Blood samples were collected at 0, 1-15, 17, 21, and 24 h and 2, 3, 4, 7, 10, 14, 17, 21, and 28 d after 13C-phytoene consumption. Plasma unlabeled and plasma-labeled phytoene concentrations were determined using ultra-HPLC quadrupole time-of-flight mass spectrometry, and data were fit to a 7-compartment carotenoid kinetic model using WinSAAM 3.0.7 software. Results: Subjects were compliant with a controlled phytoene diet, consuming a mean SE of 2.5 +/- 0.6 mg/d, resulting in a plasma unlabeled phytoene concentration of 71 +/- 14 nmol/L. A maximal plasma C-13-phytoene concentration of 55.6 +/- 5.9 nM was achieved 19.8 +/- 9.2 h after consumption, and the plasma half-life was 2.3 +/- 0.2 d. Compared with previous results for lycopene, phytoene bioavailability was nearly double at 58% +/- 19%, the clearance rate from chylomicrons was slower, and the rates of deposition into and utilization by the slow turnover tissue compartment were nearly 3 times greater. Conclusions: Although only differing from lycopene by 4 double bonds, phytoene exhibits markedly different kinetic characteristics in human plasma, providing insight into metabolic processes contributing to phytoene enrichment in plasma and tissues compared with lycopene. This trial was registered at clinicaltrials.gov as NCT01692340. C1 [Moran, Nancy E.; Riedl, Kenneth M.; Grainger, Elizabeth M.; Schwartz, Steven J.; Clinton, Steven K.] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA. [Cichon, Morgan J.; Riedl, Kenneth M.; Schwartz, Steven J.] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA. [Clinton, Steven K.] Ohio State Univ, James Canc Hosp, Columbus, OH 43210 USA. [Clinton, Steven K.] Ohio State Univ, Dept Internal Med, Div Med Oncol, Columbus, OH 43210 USA. [Novotny, Janet A.] USDA, Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Rogers, Randy B.; Erdman, John W., Jr.] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Rogers, Randy B.; Erdman, John W., Jr.] Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA. RP Clinton, SK (reprint author), Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA.; Clinton, SK (reprint author), Ohio State Univ, James Canc Hosp, Columbus, OH 43210 USA.; Clinton, SK (reprint author), Ohio State Univ, Dept Internal Med, Div Med Oncol, Columbus, OH 43210 USA. EM Steven.Clinton@osumc.edu RI Riedl, Ken/G-8621-2014 OI Riedl, Ken/0000-0002-9020-3471 FU NIH National Center for Complementary and Alternative Medicine [R21AT005166]; James Cancer Hospital's Bionutrition and Chemoprevention Fund [310684]; Ohio State University Center for Clinical and Translational Science [UL1TR001070]; Ohio State University Comprehensive Cancer Center, its Nutrient and Phytochemical Analytic Shared Resource [P30CA016058]; Pelotonia Postdoctoral Fellowship FX Supported by the NIH National Center for Complementary and Alternative Medicine (R21AT005166), The James Cancer Hospital's Bionutrition and Chemoprevention Fund (310684), The Ohio State University Center for Clinical and Translational Science (UL1TR001070), The Ohio State University Comprehensive Cancer Center and its Nutrient and Phytochemical Analytic Shared Resource (P30CA016058), and a Pelotonia Postdoctoral Fellowship (to NEM). Some carotenoid standards were donated by BASF SE, Ludwigshafen, Germany and DSM, Heerlen, Netherlands. NR 42 TC 0 Z9 0 U1 3 U2 6 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2016 VL 146 IS 2 BP 368 EP 376 DI 10.3945/jn.115.220525 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC9RD UT WOS:000369557800024 PM 26674763 ER PT J AU Park, JB AF Park, Jae B. TI Bioavailability of Alfrutamide and Caffedymine and Their P-Selectin Suppression and Platelet -Leukocyte Aggregation Mechanisms in Mice SO JOURNAL OF NUTRITION LA English DT Article DE CVD; alfrutamide; caffedymine; platelet activation; COX inhibition ID COA-TYRAMINE N-(HYDROXYCINNAMOYL)TRANSFERASE; ATHEROSCLEROSIS; EXPRESSION; THROMBOSIS; FLOW; INFLAMMATION; ACTIVATION; DISEASE; PROTEIN; CLONING AB Background: Alfrutamide and caffedymine are phenolic amides found in plants, including garlic and cocoa. However, the bioavailability of alfrutamide and caffedymine and their effects on cardiovascular diseases (CVDs), particularly via effects on P-selectin expression(PSE) and platelet -leukocyte aggregation (PLA), are unknown. Objective: The objective of this study was to investigate the bioavailability of alfrutamide and caffedymine and their effects on PSE and PLA, which are frequently involved in the progression of CVDs. Methods: Cyclooxygenase (COX) I and COX-II activities and cAMP were determined by using COX "and cAMP kits. Bioavailability was determined by HPLC analysis of plasma samples from Swiss Webster mice orally administered alfrutamide and caffedymine (10 mu g each). PSE and PLA were also measured by flow cytometry using blood samples from the same mice. Results: At 0.05 mu mol/L, alfrutamide and caffedymine inhibited COX-I and COX-II by 20-40% (P < 0.05) and 16-33% (P < 0.05), respectively, compared with the control. At 0.1 p.mol/L, the 2 compounds also inhibited platelet PSE by 28% (P < 0.05) and 35% (P < 0.05), respectively, compared with the control. The (32-adrenoceptor antagonists IC1118551 and butoxamine partially suppressed the inhibition of PSE by caffedymine, suggesting that)32 receptors are involved in inhibition by caffedymine but not by alfrutamide. At the same concentration (0.1 pi,mol/L), however, these 2 compounds inhibited PLA by 24-32% (P < 0.05) compared with the control. In addition, mice administered caffedymine and alfrutamide orally (10 mu g/35 g body weight) exhibited maximum concentrations >0.6 p,mol/L and significant inhibition of PSE by 23-34% (P < 0.05) and PLA by 20-27% (P < 0.05) compared with control mice. Conclusions: These data show the adequate bioavailability of alfrutamide and caffedymine and their different mechanisms of suppressing PSE and PLA: alfrutamide exerts its effects only via COX inhibition, whereas caffedymine works through both COX inhibition and cAMP amplification. C1 [Park, Jae B.] USDA ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. RP Park, JB (reprint author), USDA ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. EM jae.park@ars.usda.gov FU USDA [8040-51000-057-00]; University of California, Los Angeles School of Medicine; University of Florida; American Botanical Council; American Herbal Products Association; ASN; Japanese Society for Food Factors; Japan Society for Bioscience, Biotechnology, and Agrochemistry; Japan Society of Nutrition and Food Science; Natural Products Association; Wakunaga of America Co., Ltd. FX Supported by the USDA (project 8040-51000-057-00).; Published in a supplement to The Journal of Nutrition. Presented at the conference "2014 International Garlic Symposium: Role of Garlic in Cardiovascular Disease Prevention, Metabolic Syndrome, and Immunology," held 4-6 March 2014 at St. Regis Monarch Beach Resort in Dana Point, CA. This supplement is dedicated to our colleague and friend John A Milner. His dedication to good science and his voice for nutrition are remembered and sorely missed. The symposium was sponsored by the University of California, Los Angeles School of Medicine and the University of Florida and co-sponsored by the American Botanical Council; the American Herbal Products Association; the ASN; the Japanese Society for Food Factors; the Japan Society for Bioscience, Biotechnology, and Agrochemistry; the Japan Society of Nutrition and Food Science; and the Natural Products Association. The symposium was supported by Agencias Motta S.A.; Bionam; Eco-Nutraceuticos; Healthy U 2000 Ltd.; Magna; Mannavita Bvba; MaxiPharma; Medica Nord A. S.; Nature's Farm Pte. Ltd.; Nature Valley W.L.L.; Organic Health Ltd.; Oy Valioravinto Ab; Purity Life Health Products L.P.; PT Nutriprima Jayasakti; Vitaco Health Ltd.; Vitae Natural Nutrition; Sanofi Consumer Health Care; Wakunaga Pharmaceutical Co., Ltd.; and Wakunaga of America Co., Ltd. The Chair of the conference and Scientific Program Coordinator for the supplement publication was Matthew J Budoff, Harbor-UCLA Medical Center, Torrance, CA. Scientific Program Coordinator disclosures: MJ Budoff has been awarded research grants from Wakunaga of America Co., Ltd., and received an honorarium for serving as Chair of the conference. Vice-Chair and Supplement Coordinator for the supplement publication was Susan S Percival, University of Florida, Gainesville, FL. Supplement Coordinator disclosures: SS Percival has been awarded research grants from Wakunaga of America Co., Ltd., and received an honorarium for serving as Vice-Chair of the conference. Publication costs for this supplement were defrayed in part by the payment of page charges. This publication must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact. The opinions expressed in this publication are those of the authors and are not attributable to the sponsors or the publisher, Editor, or Editorial Board of The Journal of Nutrition. NR 35 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2016 VL 146 IS 2 BP 437S EP 443S DI 10.3945/jn.114.202473 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC9RD UT WOS:000369557800036 PM 26764323 ER PT J AU Charron, CS Dawson, HD Novotny, JA AF Charron, Craig S. Dawson, Harry D. Novotny, Janet A. TI Garlic Influences Gene Expression In Vivo and In Vitro SO JOURNAL OF NUTRITION LA English DT Article DE garlic; cardiovascular disease; cancer; allyl methyl sulfide; dailyl disulfide; diallyl trisulfide; S-allylcysteine ID GLUTATHIONE-S-TRANSFERASE; TOKUSHIMA FATTY RATS; ORGANOSULFUR COMPOUNDS; DIALLYL DISULFIDE; PI-CLASS; ALLYL CYSTEINE; LIVER-DISEASE; CANCER CELLS; INDUCTION; APOPTOSIS AB There is a large body of preclinical research aimed at understanding the roles of garlic and garlic-derived preparations in the promotion of human health. Most of this research has targeted the possible functions of garlic in maintaining cardiovascular health and in preventing and treating cancer. A wide range of outcome variables has been used to investigate the bioactivity of garlic, ranging from direct measures of health status such as cholesterol concentrations, blood pressure, and changes in tumor size and number, to molecular and biochemical measures such as mRNA gene expression, protein concentration, enzyme activity, and histone acetylation status. Determination of how garlic influences mRNA gene expression has proven to be a valuable approach to elucidating the mechanisms of garlic bioactivity. Preclinical studies investigating the health benefits of garlic far outnumber human studies and have made frequent use of mRNA gene expression measurement. There is an immediate need to understand mRNA gene expression in humans as well. Although safety and ethical constraints limit the types of available human tissue, peripheral whole blood is readily accessible, and measuring mRNA gene expression in whole blood may provide a unique window to understanding how garlic intake affects human health. C1 [Charron, Craig S.; Dawson, Harry D.; Novotny, Janet A.] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. RP Novotny, JA (reprint author), USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. EM janet.novotny@ars.usda.gov FU USDA; University of California, Los Angeles School of Medicine; University of Florida; American Botanical Council; American Herbal Products Association; ASN; Japanese Society for Food Factors; Japan Society for Bioscience, Biotechnology, and Agrochemistry; Japan Society of Nutrition and Food Science; Natural Products Association; Wakunaga of America Co., Ltd. FX CSC, HDD, and JAN were supported by the USDA.; Published in a supplement to The Journal of Nutrition. Presented at the conference "2014 International Garlic Symposium: Role of Garlic in Cardiovascular Disease Prevention, Metabolic Syndrome, and Immunology," held 4-6 March 2014 at St. Regis Monarch Beach Resort in Dana Point, CA. This supplement is dedicated to our colleague and friend John A Milner His dedication to good science and his voice for nutrition are remembered and sorely missed. The symposium was sponsored by the University of California, Los Angeles School of Medicine and the University of Florida and co-sponsored by the American Botanical Council; the American Herbal Products Association; the ASN; the Japanese Society for Food Factors; the Japan Society for Bioscience, Biotechnology, and Agrochemistry; the Japan Society of Nutrition and Food Science; and the Natural Products Association. The symposium was supported by Agencies Motta S.A.; Bionam; Eco-Nutraceuticos; Healthy U 2000 Ltd.; Magna; Mannavita Bvba; MaxiPharma; Medica Nord A.S.; Nature's Farm Pte. Ltd.; Nature Valley W.L.L.; Organic Health Ltd.; Oy Valioravinto Ab; Purity Life Health Products L.P.; PT Nutriprima Jayasakti; Vitaco Health Ltd.; Vitae Natural Nutrition; Sanofi Consumer Health Care; Wakunaga Pharmaceutical Co., Ltd.; and Wakunaga of America Co., Ltd. The Chair of the conference and Scientific Program Coordinator for the supplement publication was Matthew J Budoff, Harbor-UCLA Medical Center, Torrance, CA. Scientific Program Coordinator disclosures: MJ Budoff has been awarded research grants from Wakunaga of America Co., Ltd., and received an honorarium for serving as Chair of the conference. Vice-Chair and Supplement Coordinator for the supplement publication was Susan S Percival, University of Florida, Gainesville, FL. Supplement Coordinator disclosures: SS Percival has been awarded research grants from Wakunaga of America Co., Ltd., and received an honorarium for serving as Vice-Chair of the conference. Publication costs for this supplement were defrayed in part by the payment of page charges. This publication must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact. The opinions expressed in this publication are those of the authors and are not attributable to the sponsors or the publisher, Editor, or Editorial Board of The Journal of Nutrition. NR 36 TC 0 Z9 0 U1 2 U2 14 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2016 VL 146 IS 2 BP 444S EP 449S DI 10.3945/jn.114.202481 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC9RD UT WOS:000369557800037 PM 26764328 ER PT J AU Fofanova, TY Petrosino, JF Kellermayer, R AF Fofanova, Tatiana Y. Petrosino, Joseph F. Kellermayer, Richard TI \Microbiome-Epigenome Interactions and the Environmental Origins of Inflammatory Bowel Diseases SO JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LA English DT Review DE Crohn disease; developmental origins; DNA methylation; environment; epidemiology; inflammatory bowel disease; microbiome; nutrition; ulcerative colitis ID CLOSTRIDIUM-DIFFICILE INFECTION; PEDIATRIC ULCERATIVE-COLITIS; BUTYRATE-PRODUCING BACTERIA; POPULATION-BASED COHORT; COLD CHAIN HYPOTHESIS; AMBIENT AIR-POLLUTION; ONSET CROHNS-DISEASE; GUT MICROBIOTA; DNA METHYLATION; INTESTINAL MICROBIOTA AB The incidence of pediatric inflammatory bowel disease (IBD), which includes Crohn disease and ulcerative colitis, has risen alarmingly in the Western and developing world in recent decades. Epidemiologic (including monozygotic twin and migrant) studies highlight the substantial role of environment and nutrition in IBD etiology. Here we review the literature supporting the developmental and environmental origins hypothesis of IBD. We also provide a detailed exploration of how the human microbiome and epigenome (primarily through DNA methylation) may be important elements in the developmental origins of IBD in both children and adults. C1 [Fofanova, Tatiana Y.; Petrosino, Joseph F.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr,Texas Childrens Hosp, Alkek Ctr Metagen & Microbiome Res,Baylor Coll Me, Houston, TX 77030 USA. [Fofanova, Tatiana Y.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Program Translat Biol & Mol Med,Baylor Coll Med,T, Houston, TX 77030 USA. [Petrosino, Joseph F.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Mol Virol & Microbiol,Baylor Coll Med,Texas, Houston, TX 77030 USA. [Kellermayer, Richard] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr,Texas Childrens Hosp, Sect Pediat Gastroenterol,Dept Pediat,Baylor Coll, Houston, TX 77030 USA. RP Kellermayer, R (reprint author), Baylor Coll Med, Sect Pediat Gastroenterol Hepatol & Nutr, 6621 Fannin St,CC1010-00, Houston, TX 77030 USA. EM kellerma@bcm.edu FU National Institute of General Medical Science [T32 GM088129]; Gutsy Kids Fund; Houston Men of Distinction FX Institutional support was provided by Baylor College of Medicine and by the National Institute of General Medical Science (award number T32 GM088129) to T.Y.F. R.K. was supported by the Gutsy Kids Fund including philanthropic donation from the Karen and Brock Wagner family, and was also supported by the Houston Men of Distinction. J.F.P. reports no conflicts of interest. NR 222 TC 1 Z9 1 U1 8 U2 18 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0277-2116 EI 1536-4801 J9 J PEDIATR GASTR NUTR JI J. Pediatr. Gastroenterol. Nutr. PD FEB PY 2016 VL 62 IS 2 BP 208 EP 219 DI 10.1097/MPG.0000000000000950 PG 12 WC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics SC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics GA DC5WE UT WOS:000369290500002 PM 26308318 ER PT J AU Siccardi, AJ Richardson, CM Dowd, MK Wedegaertner, TC Samocha, TM AF Siccardi, Anthony J., III Richardson, Cristina M. Dowd, Michael K. Wedegaertner, Tom C. Samocha, Tzachi M. TI Digestibility of Glandless Cottonseed Protein in Diets for Pacific White Shrimp, Litopenaeus vannamei SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID FISH-MEAL; PENAEUS-VANNAMEI; PRACTICAL DIETS; APPARENT DIGESTIBILITY; GOSSYPOL CONTENT; REPLACEMENT; INGREDIENTS; JUVENILES; ENERGY; SQUID AB Accurate digestibility coefficients for protein, energy, and lipid are needed by feed formulators to optimize diets to meet nutritional requirements and to substitute ingredients cost-effectively. Of particular interest is protein, which accounts for the majority of shrimp feed content and expense. The current study evaluated seven cottonseed meal and protein products. Most of the samples were derived from a glandless cotton variety that lacks significant levels of the antinutritive compound, gossypol. The various protein fractions were evaluated for apparent crude protein, crude lipid, and energy digestibility when fed to juvenile Pacific White Shrimp, Litopenaeus vannamei. Apparent energy digestibility for the protein fractions derived from the glandless seed ranged from 76.7% for ground kernels to 94.6% for protein isolate, and these values were greater than the value obtained for the commercial meal that contained gossypol (64%). Apparent protein digestibility for the six glandless-cotton-based samples varied from 72.3% for the ground full-fat kernels to 94.1% for the protein isolate, and these values were mostly higher than the value obtained for the commercial meal (82.3%). The high apparent digestibility values demonstrate that low-gossypol cottonseed protein products may be useful as a cost-effective replacement for more expensive protein sources in L. vannamei diets. C1 [Siccardi, Anthony J., III; Richardson, Cristina M.; Samocha, Tzachi M.] Texas A&M AgriLife Res Mariculture Lab, Flour Bluff, TX 78418 USA. [Dowd, Michael K.] USDA Agr Res Serv, So Reg Res Ctr, New Orleans, LA 70124 USA. [Wedegaertner, Tom C.] Cotton Inc, Cary, NC 27513 USA. RP Siccardi, AJ (reprint author), Texas A&M AgriLife Res Mariculture Lab, Flour Bluff, TX 78418 USA. EM asiccardi@ag.tamu.edu FU Cotton Incorporated; Texas A&M AgriLife Research FX Special thanks to Kelsey Holmes for her assistance with water quality analysis. This publication was supported by Cotton Incorporated and Texas A&M AgriLife Research. NR 37 TC 1 Z9 1 U1 3 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD FEB PY 2016 VL 47 IS 1 BP 97 EP 106 DI 10.1111/jwas.12252 PG 10 WC Fisheries SC Fisheries GA DC4EI UT WOS:000369172800009 ER PT J AU Shriner, SA VanDalen, KK Root, JJ Sullivan, HJ AF Shriner, Susan A. VanDalen, Kaci K. Root, J. Jeffrey Sullivan, Heather J. TI Evaluation and optimization of a commercial blocking ELISA for detecting antibodies to influenza A virus for research and surveillance of mallards SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Influenza; ELISA; Wild birds; Surveillance ID LINKED IMMUNOSORBENT ASSAYS; AVIAN INFLUENZA; WILD BIRDS; INFECTION; DYNAMICS; POULTRY; HOST; PIGS AB The availability of a validated commercial assay is an asset for any wildlife investigation. However, commercial products are often developed for use in livestock and are not optimized for wildlife. Consequently, it is incumbent upon researchers and managers to apply commercial products appropriately to optimize program outcomes. We tested more than 800 serum samples from mallards for antibodies to influenza A virus with the IDEXX AI MultiS-Screen Ab test to evaluate assay performance. Applying the test per manufacturer's recommendations resulted in good performance with 84% sensitivity and 100% specificity. However, performance was improved to 98% sensitivity and 98% specificity by increasing the recommended cut-off. Using this alternative threshold for identifying positive and negative samples would greatly improve sample classification, especially for field samples collected months after infection when antibody titers have waned from the initial primary immune response. Furthermore, a threshold that balances sensitivity and specificity reduces estimation bias in seroprevalence estimates. Published by Elsevier B.V. C1 [Shriner, Susan A.; VanDalen, Kaci K.; Root, J. Jeffrey; Sullivan, Heather J.] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Wildlife Serv, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Shriner, SA (reprint author), US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Wildlife Serv, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM susan.a.shriner@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182 FU U.S. Department of Agriculture FX This study was funded by the U.S. Department of Agriculture. The opinions and conclusions of this article are those of the authors and do necessarily represent those of the U.S. Department of Agriculture. The mention of commercial products is for identification purposes only and does not constitute endorsement or censure. We thank K. Pedersen of Wildlife Services/U.S. Department of Agriculture for providing us with serum samples from wild mallards. NR 24 TC 4 Z9 4 U1 2 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD FEB PY 2016 VL 228 BP 130 EP 134 DI 10.1016/j.jviromet.2015.11.021 PG 5 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA DD1JY UT WOS:000369679200021 PM 26645952 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from USDA and OLAW SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Bethesda, MD USA. [Gipson, Chester] USDA, APHIS, AC, Washington, DC USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,HHS, Bethesda, MD USA. NR 3 TC 0 Z9 0 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD FEB PY 2016 VL 45 IS 2 BP 59 EP 59 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA DC6TI UT WOS:000369352100017 PM 26814350 ER PT J AU Poudyal, NC Elkins, D Nibbelink, N Cordell, HK Gyawali, B AF Poudyal, Neelam C. Elkins, Duncan Nibbelink, Nathan Cordell, H. Ken Gyawali, Buddhi TI An exploratory spatial analysis of projected hotspots of population growth, natural land loss, and climate change in the conterminous United States SO LAND USE POLICY LA English DT Article DE Landuse; Footprint; Spatial autocorrelation; Vulnerability; At-risk species; Communties; RPA assessment ID ECONOMIC-GROWTH; PROTECTED AREAS; VULNERABILITY; AMENITIES; ECOSYSTEMS; PATTERNS; PLANS AB Considering expected population growth and changes in landuse and climate, it is important to understand whether and where the relative change in one or more of these stressors will most likely impact natural and human systems. This study employed exploratory spatial data analysis of population growth, loss of natural land, and climate change data, all projected at county level for the conterminous United States as part of the USDA Forest Service's 2010 RPA Assessment. Location and geographical extent of projected significant "hotspots" of individual and multiple stressors were analyzed with respect to the location of at-risk natural ecosystems (protected lands, imperiled species) and the social and economic characteristics of human population within and outside the hotspots. While hotspots of projected climate change are broadly distributed across the Southwest, Northern Midwest, and Northeast, hotspots of projected natural land loss clustered in the Southeast and Pacific Northwest, and the primary area of overlap for multiple changes was the Southwest. Most counties in hotspots are currently home to higher numbers of at -risk or listed threatened and endangered (T&E) species than those outside. Also, up to three-quarters of the lands stringently managed to preserve their unique natural and scientific values are projected to be in hotspots of one or more stressors. Findings will be useful in identifying areas in which the greatest emerging land use management challenges will likely be concentrated due to change in one or more stressors. Findings will also inform land use planning decisions, and most importantly, provide information that will prioritize limited resources for mitigation, restoration, and management in areas of highest need. While our analysis shows notable differences in social and economic characteristics of counties projected to be in and outside hotspots, it also suggests the need for an in-depth study to compare sensitivity and adaptive capacity at community scale to compare the vulnerability of communities. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Poudyal, Neelam C.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Elkins, Duncan; Nibbelink, Nathan] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Cordell, H. Ken] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. [Gyawali, Buddhi] Kentucky State Univ, Coll Agr Food Sci & Sustainable Syst, Frankfort, KY 40601 USA. RP Poudyal, NC (reprint author), Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. EM npoudyal@utk.edu OI Elkins, Duncan/0000-0001-5798-5873; Poudyal, Neelam/0000-0003-1230-0679 FU USDA Forest Service, Southern Research Station FX The authors wish to acknowledge that this analysis is based in part on data and the research that underpins those data from the USDA Forest Service's 2010 Resources Planning Act (RPA) National Assessment of Forest and Rangeland Resources (see the summary report at http://www.fs.fed.us/research/publications/gtr/gtr_wo87.pdf). Teams of Forest Service and university scientists compiled the most current data and developed models to project key indicators of forest and range resource conditions. We especially would like to acknowledge the Forest Service team leads Dr. David Wear (land use projections), Dr. Stanley Zarnoch (population projections), Dr. Curtis Flather (wildlife projections), Dr. Kurt Ritters (land cover projections) and Dr. Linda Joyce (climate projections). We would also like to acknowledge funding support provided by the USDA Forest Service, Southern Research Station. NR 36 TC 1 Z9 1 U1 8 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-8377 EI 1873-5754 J9 LAND USE POLICY JI Land Use Pol. PD FEB PY 2016 VL 51 BP 325 EP 334 DI 10.1016/j.landusepol.2015.11.021 PG 10 WC Environmental Studies SC Environmental Sciences & Ecology GA DC9QI UT WOS:000369555700030 ER PT J AU Byrdwell, WC AF Byrdwell, William Craig TI The Simulacrum System as a Construct for Mass Spectrometry of Triacylglycerols and Others SO LIPIDS LA English DT Article DE APCI-MS; Triacylglycerols; Triglycerides; Regioisomers; Simulacrum; Mass spectrometry ID PERFORMANCE LIQUID-CHROMATOGRAPHY; DIETARY-SUPPLEMENT; VEGETABLE-OILS; UPDATED BOTTOM; FATTY-ACIDS; VITAMIN-D; TRIGLYCERIDES; REGIOISOMERS AB A construct called a simulacrum is defined that provides all possible solutions to a sum of two mass spectral abundances, based on values (abundances) or ratios of those values. The defined construct is applied to atmospheric pressure chemical ionization mass spectrometry (MS) of triacylglycerols (TAG). A simulacrum has precisely defined components, specifically a simulacrum sum, four Possibilities to Observe, two Cases, and eight solutions. A simulacrum with no restrictions is the First General Form of a Simulacrum. When one value is specified to be 1 (as in MS), the construct is called a Unit Simulacrum, also called the First Specified Form of a Simulacrum. When one value is 1 and no value can be greater than 1 (the two specifications dictated by mass spectrometry), the construct is called the Second Specified Form of a Simulacrum, or the Mass Spectrometry Simulacrum. Simulacra are used with three Critical Ratios calculated from raw abundances in mass spectra of TAG to provide structural information about the degree of unsaturation in TAG, the identity and quantity of regioisomers, and other structural characteristics. Three-level-deep nested simulacrum solutions yield the recently reported Updated Bottom Up Solution, from which the protonated molecule, [MH](+), and all diacylglycerol-like fragments, [DAG](+), of TAG can be reproduced from the Critical Ratios. Thus, the simulacrum solutions constitute a reduced data set in which more information is provided in fewer values than raw abundances, such that the Critical Ratios constitute a compact library of mass spectra. C1 [Byrdwell, William Craig] USDA ARS, Food Composit & Methods Dev Lab, 10300 Baltimore Ave,Bldg 161, Beltsville, MD 20705 USA. RP Byrdwell, WC (reprint author), USDA ARS, Food Composit & Methods Dev Lab, 10300 Baltimore Ave,Bldg 161, Beltsville, MD 20705 USA. EM C.Byrdwell@ars.usda.gov FU USDA Agricultural Research Service FX This work was supported by the USDA Agricultural Research Service. Mention or use of specific products or brands do not represent or imply endorsement by the USDA. NR 22 TC 0 Z9 0 U1 6 U2 8 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD FEB PY 2016 VL 51 IS 2 BP 211 EP 227 DI 10.1007/s11745-015-4101-1 PG 17 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DC5SL UT WOS:000369280600008 PM 26662048 ER PT J AU Govindjee Prince, RC Ort, DR AF Govindjee Prince, Roger C. Ort, Donald R. TI Colin A. Wraight, 1945-2014 SO PHOTOSYNTHESIS RESEARCH LA English DT Biographical-Item DE Chemiosmosis; Electron transfer; Proton motive force; Quantum yield of primary photochemistry; Rhodobacter sphaeroides; Two-electron gate ID PHOTOSYNTHETIC REACTION CENTERS; BACTERIAL REACTION CENTERS; SCANNING OPTICAL MICROSCOPY; ACCEPTOR QUINONE COMPLEX; INDUCED H+ BINDING; RHODOBACTER-SPHAEROIDES; ELECTRON-TRANSFER; PROTON-TRANSFER; OXYGEN EVOLUTION; PHOTOSYSTEM-II AB Colin Allen Wraight, a central figure in photosynthetic electron transfer research since the 1970s, died in Urbana, Illinois, on July 10, 2014. Born in London, England, on November 27, 1945, he had only recently retired from his position as a Professor in Biochemistry, Biophysics & Quantitative Biology, and Plant Biology at the University of Illinois at Urbana-Champaign. Wraight was known especially for his pioneering studies on electron and proton transfer in the photochemical reaction center, and for his careful quantitation of the remarkable quantum efficiency of this device. C1 [Govindjee] Univ Illinois, Dept Plant Biol, Dept Biochem, 265 Morrill Hall,505 South Goodwin Ave, Urbana, IL 61801 USA. [Govindjee] Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA. [Prince, Roger C.] ExxonMobil Biomed Sci, Annandale, NJ 08801 USA. [Ort, Donald R.] Univ Illinois, Global Change & Photosynth Res Unit, USDA ARS, Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. RP Govindjee (reprint author), Univ Illinois, Dept Plant Biol, Dept Biochem, 265 Morrill Hall,505 South Goodwin Ave, Urbana, IL 61801 USA.; Govindjee (reprint author), Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA. EM gov@illinois.edu; roger.c.prince@exxonmobil.com; d-ort@illinois.edu OI Prince, Roger/0000-0002-5174-4216 NR 78 TC 1 Z9 1 U1 1 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0166-8595 EI 1573-5079 J9 PHOTOSYNTH RES JI Photosynth. Res. PD FEB PY 2016 VL 127 IS 2 BP 237 EP 256 DI 10.1007/s11120-015-0174-1 PG 20 WC Plant Sciences SC Plant Sciences GA DC7UD UT WOS:000369425000007 PM 26202746 ER PT J AU Stipanovic, R Puckhaber, L Frelichowski, J Esquivel, J Westbrook, J O'Neil, M Bell, A Dowd, M Hake, K Duke, S AF Stipanovic, Robert Puckhaber, Lorraine Frelichowski, James, Jr. Esquivel, Jesus Westbrook, John O'Neil, Mike Bell, Alois Dowd, Michael Hake, Kater Duke, Sara TI Gossypolhemiquinone, a dimeric sesquiterpenoid identified in cotton (Gossypium) SO PHYTOCHEMISTRY LA English DT Article DE Gossypium thurberi; Malvaceae; Insect herbivory resistance; Di-sesquiterpenoid; Helicoverpa zea; Gossypol; Gossypolhemiquinone ID TERPENOID ALDEHYDES; CANCER CELLS; GOSSYPOLONE; (-)-GOSSYPOL; RESISTANCE; GROWTH AB The report that the cotton leaf perforator, Bucculatrix thurberiella, is one of the few insect herbivores to attack Gossypium thurberi prompted an investigation of the terpenoids present in the leaves of this wild species of cotton. Members of Gossypium produce subepidermal pigment glands in their leaves that contain the dimeric sesquiterpenoid gossypol as well as other biosynthetically related terpenoids. In addition to gossypol, a previously unknown dimeric sesquiterpenoid, gossypolhemiquinone (GHQ), was identified in trace amounts in G. thurberi, a member of the D genome. Other members of the D genome of Gossypium were subsequently found to contain this compound, but GHQ was not detected in commercial cotton cultivars. When fed to Helicoverpa zea in an artificial diet, GHQ delayed days -to -pupation, reduced pupal weights, and survival to adulthood to a lesser or equal extent than gossypol in comparison to the control diet. However, GHQ had a synergistic effect on survival and days -to -pupation when combined with gossypol at the highest dosage tested (0.18%; 15.5:84.5 GHQ:gossypol). Because gossypol exhibits anticancer activity, GHQ was also evaluated for its anti -cancer activity against the National Cancer Institute's 60 -Human Tumor Cell Line Screen. Significant inhibitory activity against most of these cell lines was not observed, but the results may offer some promise against leukemia cancer cell lines. Published by Elsevier Ltd. C1 [Stipanovic, Robert; Puckhaber, Lorraine; Frelichowski, James, Jr.; Esquivel, Jesus; Westbrook, John; O'Neil, Mike; Bell, Alois; Duke, Sara] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Dowd, Michael] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Hake, Kater] Cotton Inc, 6399 Weston Pkwy, Cary, NC 27513 USA. RP Stipanovic, R (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. EM bob.stipanovic@ars.usda.gov FU Cotton Inc. FX We thank Howard Williams of Texas A&M University for providing NMR spectra, and Stephanie Sullivan and Christine Soriaga for excellent technical assistance. We thank Cotton Inc. for partial support for this work. Kathy Knighten of Mississippi State University provided H. zea eggs from which larvae were reared for the insect feeding study. NR 22 TC 0 Z9 0 U1 7 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9422 J9 PHYTOCHEMISTRY JI Phytochemistry PD FEB PY 2016 VL 122 BP 165 EP 171 DI 10.1016/j.phytochem.2015.12.009 PG 7 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DC8HJ UT WOS:000369460200017 PM 26725485 ER PT J AU Lou, Y Davis, AS Yannarell, AC AF Lou, Yi Davis, Adam S. Yannarell, Anthony C. TI Interactions between allelochemicals and the microbial community affect weed suppression following cover crop residue incorporation into soil SO PLANT AND SOIL LA English DT Article DE Allelochemicals; Isoflavones; Red clover (Trifolium pratense L.); Soil microbes; Weed suppression; Wild mustard (Sinapis alba L.) ID CLOVER TRIFOLIUM-PRATENSE; LEAST-SQUARES REGRESSION; MUSTARD SEEDLING GROWTH; RED-CLOVER; ALLELOPATHIC INTERACTIONS; BIOLOGICAL-CONTROL; PLANT-PATHOGENS; CRIMSON CLOVER; MANAGEMENT; SYSTEMS AB The objective of this study is to understand how soil microorganisms interact with cover crop-derived allelochemicals to suppress weed germination and growth following cover crop residue incorporation. We conducted a time series experiment crossing sterilized and non-sterilized soil with four different residue treatments. We measured weed seed germination rates, radicle elongation, and disease incidence in seed germination bioassays. We also monitored cover crop-derived, isoflavone allelochemicals in these bioassays. We partitioned the total weed suppression into three sources: microbe-only inhibition, residue-only inhibition, and the microbe-residue interaction. Microbial activity suppressed weed germination and growth for 30 days, while cover crop-derived allelochemicals provided suppression for a limited time. There was an antagonistic interaction between microbes and allelochemicals. This interaction was strongest for water-soluble allelochemicals, while residue fractions containing intact plant tissues retained greater suppressiveness even in the presence of a live microbial community. Microbial activity can directly suppress weed germination and growth, but microorganisms also indirectly help weeds by degrading cover crop-derived allelochemicals. As a result of these interactions, cover crop-derived weed suppression in agricultural soils shifts from an early allelochemical-dominated phase to a later phase where microbial suppression is more important. C1 [Lou, Yi; Yannarell, Anthony C.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL USA. [Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL USA. RP Yannarell, AC (reprint author), Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL USA. EM acyann@illinois.edu FU USDA-CSREES [ILLU-875-317]; USDA-NIFA (Agriculture and Food Research Initiative Climate Change Mitigation and Adaptation in Agriculture Grant) [2011-67003-30343] FX We thank A. Davis, A. Kent, A. Leakey, C. Soman, D. Li, A. Beck, D. Cohen, N. Stevenson, M. Scott, G. Rienne, and S. Chavez for valuable feedback during the preparation of this manuscript. This research was funded by the USDA-CSREES (Project ILLU-875-317) and by the USDA-NIFA (Agriculture and Food Research Initiative Climate Change Mitigation and Adaptation in Agriculture Grant 2011-67003-30343). NR 65 TC 0 Z9 0 U1 16 U2 54 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD FEB PY 2016 VL 399 IS 1-2 BP 357 EP 371 DI 10.1007/s11104-015-2698-8 PG 15 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA DC7UG UT WOS:000369425300027 ER PT J AU Zhu, XB Gong, HL He, QY Zeng, ZX Busse, JS Jin, WW Bethke, PC Jiang, JM AF Zhu, Xiaobiao Gong, Huiling He, Qunyan Zeng, Zixian Busse, James S. Jin, Weiwei Bethke, Paul C. Jiang, Jiming TI Silencing of vacuolar invertase and asparagine synthetase genes and its impact on acrylamide formation of fried potato products SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE acrylamide; reducing sugars; asparagine; gene silencing; potato ID FREE AMINO-ACIDS; ARABIDOPSIS-THALIANA; MAILLARD REACTION; FRENCH FRIES; VARIETIES; SUGARS; FOODS; METABOLISM; PRECURSORS; EXPRESSION AB Acrylamide is produced in a wide variety of carbohydrate-rich foods during high-temperature cooking. Dietary acrylamide is a suspected human carcinogen, and health concerns related to dietary acrylamide have been raised worldwide. French fries and potato chips contribute a significant proportion to the average daily intake of acrylamide, especially in developed countries. One way to mitigate health concerns related to acrylamide is to develop potato cultivars that have reduced contents of the acrylamide precursors asparagine, glucose and fructose in tubers. We generated a large number of silencing lines of potato cultivar Russet Burbank by targeting the vacuolar invertase gene VInv and the asparagine synthetase genes StAS1 and StAS2 with a single RNA interference construct. The transcription levels of these three genes were correlated with reducing sugar (glucose and fructose) and asparagine content in tubers. Fried potato products from the best VInv/StAS1/StAS2-triple silencing lines contained only one-fifteenth of the acrylamide content of the controls. Interestingly, the extent of acrylamide reduction of the best triple silencing lines was similar to that of the best VInv-single silencing lines developed previously from the same potato cultivar Russet Burbank. These results show that an acrylamide mitigation strategy focused on developing potato cultivars with low reducing sugars is likely to be an effective and sufficient approach for minimizing the acrylamide-forming potential of French fry processing potatoes. C1 [Zhu, Xiaobiao; Gong, Huiling; He, Qunyan; Zeng, Zixian; Busse, James S.; Bethke, Paul C.; Jiang, Jiming] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Gong, Huiling] Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China. [He, Qunyan; Jin, Weiwei] China Agr Univ, Beijing Key Lab Crop Genet Improvement, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China. [Busse, James S.; Bethke, Paul C.] USDA, Vegetable Crops Res Unit, Madison, WI USA. RP Bethke, PC; Jiang, JM (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Bethke, PC (reprint author), USDA, Vegetable Crops Res Unit, Madison, WI USA. EM paul.bethke@ars.usda.edu; jjiang1@wisc.edu FU USDA NIFA Specialty Crop Research Initiative; Hatch funds FX We thank Pudota Bhaskar for assistance of RNAi construct development and the staff at the Hancock Agricultural Research Station (HARS), University of Wisconsin-Madison, for helping with planting and field management. This research was supported by the USDA NIFA Specialty Crop Research Initiative to P.C.B. and J.J. and Hatch funds to J.J. NR 43 TC 3 Z9 3 U1 6 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD FEB PY 2016 VL 14 IS 2 BP 709 EP 718 DI 10.1111/pbi.12421 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DC5TA UT WOS:000369282100027 PM 26079224 ER PT J AU Kohler, IH Macdonald, AJ Schnyder, H AF Koehler, Iris H. Macdonald, Andy J. Schnyder, Hans TI Last-Century Increases in Intrinsic Water-Use Efficiency of Grassland Communities Have Occurred over a Wide Range of Vegetation Composition, Nutrient Inputs, and Soil pH SO PLANT PHYSIOLOGY LA English DT Article ID CARBON-ISOTOPE DISCRIMINATION; TEMPERATE SEMINATURAL GRASSLAND; ATMOSPHERIC CO2 CONCENTRATION; ALUMINUM TOLERANCE; SPECIES RICHNESS; WHEAT GENOTYPES; PLANTS; VARIABILITY; DELTA-C-13; DIOXIDE AB Last-century climate change has led to variable increases of the intrinsic water-use efficiency (W-i; the ratio of net CO2 assimilation to stomatal conductance for water vapor) of trees and C-3 grassland ecosystems, but the causes of the variability are not well understood. Here, we address putative drivers underlying variable W-i responses in a wide range of grassland communities. W-i was estimated from carbon isotope discrimination in archived herbage samples from 16 contrasting fertilizer treatments in the Park Grass Experiment, Rothamsted, England, for the 1915 to 1929 and 1995 to 2009 periods. Changes in W-i were analyzed in relation to nitrogen input, soil pH, species richness, and functional group composition. Treatments included liming as well as phosphorus and potassium additions with or without ammonium or nitrate fertilizer applications at three levels. W-i increased between 11% and 25% (P < 0.001) in the different treatments between the two periods. None of the fertilizers had a direct effect on the change of W-i (Delta W-i). However, soil pH (P < 0.05), species richness (P < 0.01), and percentage grass content (P < 0.01) were significantly related to Delta W-i. Grass-dominated, species-poor plots on acidic soils showed the largest Delta W-i (+14.7 mu mol mol(-1)). The Delta W-i response of these acidic plots was probably related to drought effects resulting from aluminum toxicity on root growth. Our results from the Park Grass Experiment show that W-i in grassland communities consistently increased over a wide range of nutrient inputs, soil pH, and plant community compositions during the last century. C1 [Koehler, Iris H.; Schnyder, Hans] Tech Univ Munich, Lehrstuhl Grunlandlehre, D-85350 Freising Weihenstephan, Germany. [Macdonald, Andy J.] Rothamsted Res, Sustainable Soils & Grassland Syst Dept, Harpenden AL5 2JQ, Herts, England. [Koehler, Iris H.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. RP Schnyder, H (reprint author), Tech Univ Munich, Lehrstuhl Grunlandlehre, D-85350 Freising Weihenstephan, Germany. EM schnyder@wzw.tum.de RI Schnyder, Hans/B-7089-2011 OI Schnyder, Hans/0000-0002-0139-7535 FU Deutsche Forschungsgemeinschaft [SCHN 557/5-1, SCHN 557/8-1]; Experimentation in Ecosystem Research; European Union's Seventh Framework Program [262060]; Biotechnology and Biological Sciences Research Council (Institute National Capabilities program grant) FX This work was supported by the Deutsche Forschungsgemeinschaft (grant nos. SCHN 557/5-1 and SCHN 557/8-1), by Experimentation in Ecosystem Research, the European Union's Seventh Framework Program (grant no. 262060), and by the Biotechnology and Biological Sciences Research Council (Institute National Capabilities program grant). NR 52 TC 0 Z9 0 U1 7 U2 16 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD FEB PY 2016 VL 170 IS 2 BP 881 EP 890 DI 10.1104/pp.15.01472 PG 10 WC Plant Sciences SC Plant Sciences GA DC6PY UT WOS:000369343300022 PM 26620525 ER PT J AU Zhu, SY Eclarinal, J Baker, MS Li, G Waterland, RA AF Zhu, Shaoyu Eclarinal, Jesse Baker, Maria S. Li, Ge Waterland, Robert A. TI Developmental programming of energy balance regulation: is physical activity more 'programmable' than food intake? SO PROCEEDINGS OF THE NUTRITION SOCIETY LA English DT Article; Proceedings Paper CT Meeting of the Scottish-Section of the Nutrition-Society / Conference on Diet, Gene Regulation and Metabolic Disease CY MAR 25-26, 2015 CL Robert Gordon Univ, Aberdeen, SCOTLAND SP Nutr Soc, Scottish Sect HO Robert Gordon Univ DE Metabolic imprinting; Epigenetics; DNA methylation; Nutrition ID GENE-EXPRESSION; DNA METHYLATION; VOLUNTARY EXERCISE; FEMALE MICE; OBESITY; NUTRITION; RESTRICTION; BEHAVIOR; EXPENDITURE; MECHANISMS AB Extensive human and animal model data show that environmental influences during critical periods of prenatal and early postnatal development can cause persistent alterations in energy balance regulation. Although a potentially important factor in the worldwide obesity epidemic, the fundamental mechanisms underlying such developmental programming of energy balance are poorly understood, limiting our ability to intervene. Most studies of developmental programming of energy balance have focused on persistent alterations in the regulation of energy intake; energy expenditure has been relatively underemphasised. In particular, very few studies have evaluated developmental programming of physical activity. The aim of this review is to summarise recent evidence that early environment may have a profound impact on establishment of individual propensity for physical activity. Recently, we characterised two different mouse models of developmental programming of obesity; one models fetal growth restriction followed by catch-up growth, and the other models early postnatal overnutrition. In both studies, we observed alterations in body-weight regulation that persisted to adulthood, but no group differences in food intake. Rather, in both cases, programming of energy balance appeared to be due to persistent alterations in energy expenditure and spontaneous physical activity (SPA). These effects were stronger in female offspring. We are currently exploring the hypothesis that developmental programming of SPA occurs via induced sex-specific alterations in epigenetic regulation in the hypothalamus and other regions of the central nervous system. We will summarise the current progress towards testing this hypothesis. Early environmental influences on establishment of physical activity are likely an important factor in developmental programming of energy balance. Understanding the fundamental underlying mechanisms in appropriate animal models will help determine whether early life interventions may be a practical approach to promote physical activity in man. C1 [Zhu, Shaoyu; Eclarinal, Jesse; Baker, Maria S.; Waterland, Robert A.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Li, Ge] Cornell Univ, Weill Cornell Med Coll, Dept Biochem, New York, NY 10021 USA. [Waterland, Robert A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. RP Waterland, RA (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA.; Waterland, RA (reprint author), Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. EM waterland@bcm.edu NR 44 TC 3 Z9 3 U1 2 U2 16 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0029-6651 EI 1475-2719 J9 P NUTR SOC JI Proc. Nutr. Soc. PD FEB PY 2016 VL 75 IS 1 BP 73 EP 77 DI 10.1017/S0029665115004127 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DC7RU UT WOS:000369418800008 ER PT J AU Magney, TS Vierling, LA Eitel, JUH Huggins, DR Garrity, SR AF Magney, Troy S. Vierling, Lee A. Eitel, Jan U. H. Huggins, David R. Garrity, Steven R. TI Response of high frequency Photochemical Reflectance Index (PRI) measurements to environmental conditions in wheat SO REMOTE SENSING OF ENVIRONMENT LA English DT Article; Proceedings Paper CT 2014 ForestSAT Conference CY NOV 04-07, 2014 CL Riva del Garda, ITALY DE Photochemical Reflectance Index (PRI); Spectral Reflectance Sensors (SRS); Delta PRI; Diurnal spectral reflectance; Plant water stress; Plant nitrogen stress; Remote sensing; Xanthophyll cycle; Wheat ID LIGHT-USE EFFICIENCY; STATE CHLOROPHYLL FLUORESCENCE; RADIATION-USE EFFICIENCY; WATER-STRESS DETECTION; XANTHOPHYLL CYCLE; PHOTOSYNTHETIC EFFICIENCY; HYPERSPECTRAL IMAGERY; ENERGY-DISSIPATION; DATA ASSIMILATION; SHADOW FRACTION AB Remotely sensed data that are sensitive to rapidly changing plant physiology can provide real-time information about crop responses to abiotic conditions. The Photochemical Reflectance Index (PRI) has shown promise when measured at short timesteps to remotely estimate dynamics in xanthophyll pigment interconversion a plant photoprotective mechanism that results in lowered photosynthetic efficiency. To gain a better understanding of this dynamic spectral response to environmental conditions, we investigated PRI over two seasons (2013 and 2014) in rainfed soft white spring wheat (Triticum aestivum L.). Highly temporally resolved (measurement frequency = five minutes) in-situ radiometric measurements of PRI were collected at field plots of varying nitrogen (N) and soil water conditions (n = 16). To represent the diurnal magnitude of xanthophyll pigment inter conversion, we use a delta PRI (Delta PRI) derived from a midday PRI (xanthophyll de-epoxidation state) and an early morning PRI (xanthophyll epoxidation state). We hypothesize that Delta PRI can empirically deconvolve the diurnally changing (facultative) from the seasonally changing (constitutive) component of the PRI signal. In this study, Delta PRI demonstrated less sensitivity than an uncorrected PRI to leaf area index (LAI) and leaf chlorophyll content throughout the growing season. Delta PRI! was correlated with continuous, unattended crop responses associated with vapor pressure deficit (0.50 > R-2 > 0.48), stomatal conductance (R-2 = 0.47), and air temperature (0.42 > R-2 > 35). Further, the sensitivity with which Delta PRI responded to solar radiation under varying N treatments and periods of soil water availability (surplus, depletion, and deficit) suggests that crop growth may be inhibited by a xanthophyll cycle mediated stress response, detectable by Delta PRI A major implication of these findings is that highly temporally and spatially resolved Delta PRI data could be used to track plant status in response to changing environmental conditions. (C) 2015 Elsevier Inc. All rights reserved. C1 [Magney, Troy S.; Vierling, Lee A.; Eitel, Jan U. H.] Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA. [Vierling, Lee A.; Eitel, Jan U. H.] Univ Idaho, McCall Outdoor Sci Sch, Mccall, ID 83638 USA. [Huggins, David R.] USDA ARS, Pullman, WA 99163 USA. [Garrity, Steven R.] Decagon Devices, Pullman, WA 99163 USA. [Magney, Troy S.] NASA, Jet Prop Lab, Washington, DC USA. [Magney, Troy S.] 4800 Oak Grove Dr MS 233-300, Pasadena, CA USA. RP Magney, TS (reprint author), Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA.; Magney, TS (reprint author), NASA, Jet Prop Lab, Washington, DC USA.; Magney, TS (reprint author), 4800 Oak Grove Dr MS 233-300, Pasadena, CA USA. EM tmagney@uidaho.edu; leev@uidaho.edu; jeitel@uidaho.edu; dhuggins@wsu.edu; steven@decagon.com FU US Department of Agriculture National Institute of Food and Agricutlutre (USDA-NIFA) award [637003-3034]; NASA Idaho Space Grant Fellowship awarded [NNX10AM75H] FX Many thanks to Sam Finch, Leanna Dann, Jyotti Jennewein, and Dave Uberaga for their field support, and Drs. Barry A. Logan, Kevin L. Griffin, David Brown, Erin Brooks, Caley Gasch, Douglas R. Cobos, Colin Campbell, and Gaylon Campbell for their comments during previous versions and developments of this work. This research was made possible through the funding provided by US Department of Agriculture National Institute of Food and Agricutlutre (USDA-NIFA) award 2011 - 637003-3034 and the NASA Idaho Space Grant Fellowship awarded to TSM (#NNX10AM75H). NR 78 TC 5 Z9 5 U1 10 U2 33 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 84 EP 97 DI 10.1016/j.rse.2015.11.013 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900007 ER PT J AU Gregoire, TG Naesset, E McRoberts, RE Stahl, G Andersen, HE Gobakken, T Ene, L Nelson, R AF Gregoire, Timothy G. Naesset, Erik McRoberts, Ronald E. Stahl, Goran Andersen, Hans-Erik Gobakken, Terje Ene, Liviu Nelson, Ross TI Statistical rigor in LiDAR-assisted estimation of aboveground forest biomass SO REMOTE SENSING OF ENVIRONMENT LA English DT Article; Proceedings Paper CT 2014 ForestSAT Conference CY NOV 04-07, 2014 CL Riva del Garda, ITALY DE Sampling; Statistical inference; Variance estimation ID MODEL-BASED INFERENCE; POST-STRATIFIED ESTIMATION; GROWING STOCK VOLUME; HEDMARK COUNTY; SAMPLE SURVEY; SIMULATION APPROACH; FINITE POPULATIONS; BOOTSTRAP METHODS; AIRBORNE LIDAR; NORWAY AB For many decades remotely sensed data have been used as a source of auxiliary information when conducting regional or national surveys of forest resources. In the past decade, airborne scanning LiDAR (Light Detection and Ranging) has emerged as a promising tool for sample surveys aimed at improving estimation of aboveground forest biomass. This technology is now employed routinely in forest management inventories of some Nordic countries, and there is eager anticipation for its application to assess changes in standing biomass in vast tropical regions of the globe in concert with the UN REDD program to limit C emissions. In the rapidly expanding literature on LiDAR-assisted biomass estimation the assessment of the uncertainty of estimation varies widely, ranging from statistically rigorous to ad hoc. In many instances, too, there appears to be no recognition of different bases of statistical inference which bear importantly on uncertainty estimation. Statistically rigorous assessment of uncertainty for four large LiDAR-assisted surveys is expounded. (C) 2015 Elsevier Inc. All rights reserved. C1 [Gregoire, Timothy G.] Yale Univ, Sch Forestry & Environm Studies, 360 Prospect St, New Haven, CT 06511 USA. [Naesset, Erik; Gobakken, Terje; Ene, Liviu] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, POB 5003, NO-1432 As, Norway. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Stahl, Goran] Swedish Univ Agr Sci, Dept Forest Resource Management & Geomat, S-90183 Umea, Sweden. [Andersen, Hans-Erik] US Forest Serv, Pacific NW Res Stn, Seattle, WA 98195 USA. [Nelson, Ross] NASA, Biospher Sci Branch, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. RP Gregoire, TG (reprint author), Yale Univ, Sch Forestry & Environm Studies, 360 Prospect St, New Haven, CT 06511 USA. EM timothy.gregoire@yale.edu NR 40 TC 11 Z9 11 U1 12 U2 28 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 98 EP 108 DI 10.1016/j.rse.2015.11.012 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900008 ER PT J AU Pflugmacher, D McRoberts, RE Morsdorf, F AF Pflugmacher, Dirk McRoberts, Ronald E. Morsdorf, Felix TI Preface: ForestSAT 2014 Special Issue SO REMOTE SENSING OF ENVIRONMENT LA English DT Editorial Material C1 [Pflugmacher, Dirk] Humboldt Univ, Dept Geog, D-10099 Berlin, Germany. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN USA. [Morsdorf, Felix] Univ Zurich, Dept Geog, Zurich, Switzerland. RP Pflugmacher, D (reprint author), Humboldt Univ, Dept Geog, D-10099 Berlin, Germany. RI Morsdorf, Felix/D-1151-2010 NR 14 TC 0 Z9 0 U1 2 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 211 EP 213 DI 10.1016/j.rse.2015.11.025 PG 3 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900018 ER PT J AU Breidenbach, J McRoberts, RE Astrup, R AF Breidenbach, Johannes McRoberts, Ronald E. Astrup, Rasmus TI Empirical coverage of model-based variance estimators for remote sensing assisted estimation of stand-level timber volume SO REMOTE SENSING OF ENVIRONMENT LA English DT Article; Proceedings Paper CT 2014 ForestSAT Conference CY NOV 04-07, 2014 CL Riva del Garda, ITALY DE Forest inventory; Model-based inference; Synthetic estimator; Variance estimation; Image matching ID SMALL-AREA ESTIMATORS; FOREST INVENTORY; AERIAL IMAGES; INFERENCE AB Due to the availability of good and reasonably priced auxiliary data, the use of model-based regression-synthetic estimators for small area estimation is popular in operational settings. Examples are forest management inventories, where a linking model is used in combination with airborne laser scanning data to estimate stand-level forest parameters where no or too few observations are collected within the stand. This paper focuses on different approaches to estimating the variances of those estimates. We compared a variance estimator which is based on the estimation of superpopulation parameters with variance estimators which are based on predictions of finite population values. One of the latter variance estimators considered the spatial autocorrelation of the residuals whereas the other one did not. The estimators were applied using timber volume on stand level as the variable of interest and photogrammetric image matching data as auxiliary information. Norwegian National Forest Inventory (NFI) data were used for model calibration and independent data clustered within stands were used for validation. The empirical coverage proportion (ECP) of confidence intervals (CIs) of the variance estimators which are based on predictions of finite population values was considerably higher than the ECP of the CI of the variance estimator which is based on the estimation of superpopulation parameters. The ECP further increased when considering the spatial autocorrelation of the residuals. The study also explores the link between confidence intervals that are based on variance estimates as well as the well-known confidence and prediction intervals of regression models. 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license. C1 [Breidenbach, Johannes; Astrup, Rasmus] Norwegian Inst Bioecon Res NIBIO, Postboks 115, N-1431 As, Norway. [McRoberts, Ronald E.] US Forest Serv, USDA, Forest Inventory & Anal, St Paul, MN USA. RP Breidenbach, J (reprint author), Norwegian Inst Bioecon Res NIBIO, Postboks 115, N-1431 As, Norway. EM Johannes.Breidenbach@nibio.no FU European Union [633464] FX We would like to thank Mr. Johannes Rahlf for supporting the processing of the remote sensing data. We would like to thank Dr. Steen Magnussen for valuable comments to an early version of the manuscript. We acknowledge the comments of two anonymous reviewers that helped to improve the manuscript. Parts of this study has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 633464: DIABOLO - Distributed, Integrated and Harmonised Forest Information for Bioeconomy Outlooks. NR 40 TC 4 Z9 4 U1 2 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 274 EP 281 DI 10.1016/j.rse.2015.07.026 PG 8 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900024 PM 28148972 ER PT J AU Vogeler, JC Yang, ZQ Cohen, WB AF Vogeler, Jody C. Yang, Zhiqiang Cohen, Warren B. TI Mapping post-fire habitat characteristics through the fusion of remote sensing tools SO REMOTE SENSING OF ENVIRONMENT LA English DT Article; Proceedings Paper CT 2014 ForestSAT Conference CY NOV 04-07, 2014 CL Riva del Garda, ITALY DE Habitat; Landsat time series; Lidar; Maps; Post-fire; Snags; Shrub cover ID LANDSAT-DERIVED DISTURBANCE; LIDAR-DATA; DISTRIBUTION MODELS; BURNED LANDSCAPES; FIRE SEVERITY; BOREAL FOREST; CASCADE RANGE; WILDLIFE; WILDFIRE; BIRD AB Post-fire snags provide important resources for cavity nesting communities as well as being subject to timber removal through salvage logging practices. Tools that can characterize their distributions along with other features important as wildlife habitat, such as woody shrub cover, would be useful for research and management purposes. Three dimensional lidar data and Landsat time series disturbance products have both shown varying promise in their ability to characterize aspects of dead biomass and understory cover, but studies exploring the combination of the remote sensing datasets calibrated with field data to model difficult to map habitat components are limited. The purpose of this study was to 1) relate lidar and Landsat time series products to field collected calibration data to produce maps of important post-fire wildlife habitat components including snags of varying sizes and the availability of a woody shrub layer; and 2) compare the individual performance of the Landsat and lidar datasets for predicting the distributions of these difficult to map forest habitat features Using 164 field calibration plots and remote sensing predictors, we modeled and mapped the distributions of our response variables including snag classes (dbh >= 40 cm; >= 50 cm, and >= 75 cm) and woody shrub cover thresholds (>= 30 and >= 50% cover) at 10 m resolutions. Remote sensing predictors included various lidar structure and topography variables and Landsat time series products representing the pre-fire forest, disturbance magnitude, and current forest condition. A model was chosen for mapping purposes using AIC model selection and then by comparing leave-one-out-cross-validation error matrices to choose among competing models. We were able to predict and map all response variables with moderate accuracies and variable sensitivity (true positive) and specificity (true negative) rates. All snag and shrub models were considered to have "good" predictive performance as indicated by area under the curve values (0.74-0.91), with percent correctly classified values ranging from 69-85% when a probability threshold is chosen that balances false positive and false negative errors. Landsat models performed marginally better than lidar structure models according to classification statistics. Landsat-only models had slightly less accuracy than models that included lidar and Landsat data, but often with greater errors than the combined model. The ability to map the response variables with moderate errors and acceptable accuracies for many applications was through the fusion of these remote sensing datasets. (C) 2015 Elsevier Inc. All rights reserved. C1 [Vogeler, Jody C.; Yang, Zhiqiang; Cohen, Warren B.] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Cohen, Warren B.] US Forest Serv, PNW Res Stn, 3200 NW Jefferson Way, Corvallis, OR 97331 USA. RP Vogeler, JC (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. EM jody.vogeler@oregonstate.edu FU National Aeronautics and Space Administration, Carbon Cycle Program (NASA Grant) [10-CARBON10-45] FX Our research was funded by the National Aeronautics and Space Administration, Carbon Cycle Program (NASA Grant 10-CARBON10-45). We thank our field technicians for assisting in data collection and Keith Olsen for initial assistance in lidar processing methods. NR 44 TC 4 Z9 4 U1 12 U2 35 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 294 EP 303 DI 10.1016/j.rse.2015.08.011 PG 10 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900026 ER PT J AU Mildrexler, D Yang, ZQ Cohen, WB Bell, DM AF Mildrexler, David Yang, Zhiqiang Cohen, Warren B. Bell, David M. TI A forest vulnerability index based on drought and high temperatures SO REMOTE SENSING OF ENVIRONMENT LA English DT Article; Proceedings Paper CT 2014 ForestSAT Conference CY NOV 04-07, 2014 CL Riva del Garda, ITALY DE Forest vulnerability index; Stress, climate change; Drought; Aqua MODIS land surface temperature; Evapotranspiration; Water deficit ID LAND-SURFACE-TEMPERATURE; INDUCED TREE MORTALITY; CONTERMINOUS UNITED-STATES; CLIMATE-CHANGE; PACIFIC-NORTHWEST; DIE-OFF; VEGETATION; ECOSYSTEMS; PRECIPITATION; CONSEQUENCES AB Increasing forest stress and tree mortality has been directly linked to combinations of drought and high temperatures. The climatic changes expected during the next decades - large increases in mean temperature, increased heat waves, and significant long-term regional drying in the western USA - will likely increase chronic forest stress and mortality. The aim of this research is to develop and apply a new forest vulnerability index (FVI) associated with drought and high temperatures across the Pacific Northwest region (PNW; Oregon and Washington) of the USA during the MODIS Aqua era (since 2003). Our technique incorporates the alterations to canopy water and energy exchange processes caused by drought and high temperatures with spatially continuous MODIS land surface temperature (LST) and evapotranspiration (ET), and with Parameter-elevation Relationships on Independent Slopes Model (PRISM) precipitation (P) data. With P and ET, we calculate a monthly water balance variable for each individual pixel normalized by forest type group (FTG), and then difference the water balance with the corresponding normalized monthly meanLST to calculate a monthly forest stress index (FSI). We then extract the pixel-specific (800-m resolution) statistically significant temporal trends of the FSI from 2003 to 2012 by month (April to October). The FVI is the slope of the monthly FSI across years, such that there is a FVI for each month. Statistically significant positive slopes indicate interannual increases in stress leading to expected forest vulnerability (positive FVI) for a given month. Positive FVI values were concentrated in the months of August and September, with peak vulnerability occurring at different times for different FTGs. Overall, increased vulnerability rates were the highest in drier FTGs such as Ponderosa Pine, Juniper, and Lodgepole Pine. Western Larch and Fir/Spruce/Mountain Hemlock groups occupy moister sites but also had relatively high proportion of positive FVI values. The Douglas-fir group had the second largest total area of increased vulnerability due to its large areal extent in the study area. Based on an analysis using imagery viewed in Google Earth, we confirm that areas with increased vulnerability are associated with greater amounts of stress and mortality. The FVI is a new way to conceptualize and monitor forest vulnerability based on first-order principles and has the potential to be generalized to other geographical areas. (C) 2015 Elsevier Inc. All rights reserved. C1 [Mildrexler, David; Yang, Zhiqiang] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Cohen, Warren B.; Bell, David M.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Mildrexler, D (reprint author), Oregon State Univ, Coll Forestry, Lab Applicat Remote Sensing Ecol, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM david.mildrexler@oregonstate.edu FU USDA Forest Service, Region 6 Climate Change Program; Graduate School and the Department of Forest Ecosystems and Society at Oregon State University FX This work was supported by the USDA Forest Service, Region 6 Climate Change Program, and the Graduate School and the Department of Forest Ecosystems and Society at Oregon State University. We thank Maosheng Zhao for providing the MODIS datasets and Robert Kennedy for use of the LandTrendr data. NR 94 TC 5 Z9 5 U1 17 U2 55 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 314 EP 325 DI 10.1016/j.rse.2015.11.024 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900028 ER PT J AU Sannier, C McRoberts, RE Fichet, LV AF Sannier, Christophe McRoberts, Ronald E. Fichet, Louis-Vincent TI Suitability of Global Forest Change data to report forest cover estimates at national level in Gabon SO REMOTE SENSING OF ENVIRONMENT LA English DT Article; Proceedings Paper CT 2014 ForestSAT Conference CY NOV 04-07, 2014 CL Riva del Garda, ITALY ID ACCURACY ASSESSMENT; LAND-COVER; MAPS AB For purposes of greenhouse gas emissions (GHG) accounting, estimation of deforestation area in tropical countries often relies on satellite remote sensing in the absence of National Forest Inventories (NFI). Gabon has recently launched a National Climate Action Plan with the intent of establishing a National Forest Monitoring System that meets the Intergovernmental Panel on Climate Change (IPCC) 2006 guidelines for the Agriculture, Forestry and Other Land Use (AFOLU) sector. The assessment of areas of forest cover and forest cover change is essential to estimate activity data, defined as areas of various categories of land use change by the IPCC guidelines. An appropriately designed probability sample can be used to estimate forest cover and net change and their associated uncertainties and express them in the form of confidence intervals at selected probability thresholds as required in the IPCC 2006 guidelines and for reporting to the United Nations Framework Convention on Climate Change (UNFCCC). However, wall-to-wall mapping is often required to provide a comprehensive assessment of forest resources and as input to land use plans for management purposes, but wall-to-wall approaches are more expensive than a sample based approach based on visual interpretation and require specialized equipment and staff. The recent release of the University of Maryland (UMD) Global Forest Change (GFC) map products could be an alternative for tropical countries wishing to develop their own wall-to-wall forest map products but without the resources to do so. Therefore, the aim of this study is to assess the feasibility of replacing national wall-to-wall forest maps with forest maps obtained from the UMD GFC initiative. A model assisted regression (MAR) estimator was applied using the combination of reference data obtained from a probability sample and forest cover and forest cover change maps either (i) produced nationally or (ii) obtained from the UMD GFC data. The resulting activity data are potentially more accurate than the SRS estimate and provide an assessment of the precision of the estimate which is not available from map accuracy indices alone. Results obtained for 2000 and 2010 for both the national and UMD GFC datasets confirm the high level of forest cover in Gabon, more than 235 million ha representing approximately 88.5% of the country. Although the UMD GFC dataset provides a reliable means of producing area statistics at national level combined with appropriate sample reference data, thus offering an alternative to nationally produced datasets (i) the classification errors associated with the Global dataset have non-negligible effects on both the estimate and the precision which supports the more general statement that map data should not be used alone to produce area estimates, and (ii) the maps obtained from the UMD GFC dataset require specific calibration of the tree cover percentage representing a non -negligible effort requiring specialized staff and equipment. Guidelines on how to use and further improve UMD GFC maps for national reporting are suggested. However, this additional effort would still most likely be less than the production of national based maps. (C) 2015 Elsevier Inc All rights reserved. C1 [Sannier, Christophe; Fichet, Louis-Vincent] SIRS, Parc Cimaise 27 Rue Carrousel, F-59650 Villeneuve Dascq, France. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN USA. RP Sannier, C (reprint author), SIRS, Parc Cimaise 27 Rue Carrousel, F-59650 Villeneuve Dascq, France. EM christophe.sannier@sirs-fr.com FU European Space Agency (ESA) Global Monitoring for the Environment and Security Service Element on Forest Monitoring REDD extension project (GSE FM REDD); Fond Francais pour l'Environnement Mondial (FFEM) FX The production of forest cover and forest cover change maps was funded under the European Space Agency (ESA) Global Monitoring for the Environment and Security Service Element on Forest Monitoring REDD extension project (GSE FM REDD) coordinated by GAF AG and The GEOFORAFRI project funded by the Fond Francais pour l'Environnement Mondial (FFEM). NR 29 TC 3 Z9 3 U1 9 U2 20 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD FEB PY 2016 VL 173 BP 326 EP 338 DI 10.1016/j.rse.2015.10.032 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DC4OT UT WOS:000369200900029 ER PT J AU Petrujkic, BT Cojkic, A Petrujkic, K Jeremic, I Masulovic, D Dimitrijevic, V Savic, M Pesic, M Beier, RC AF Petrujkic, Branko T. Cojkic, Aleksandar Petrujkic, Katarina Jeremic, Ivan Masulovic, Dragan Dimitrijevic, Vladimir Savic, Mila Pesic, Miodrag Beier, Ross C. TI Transabdominal and transrectal ultrasonography of fetuses in Wurttemberg ewes: Correlation with gestational age SO ANIMAL SCIENCE JOURNAL LA English DT Article DE ewe; fetus; gestation age; ultrasound ID FETAL; SHEEP; PREGNANCY AB It is useful to determine the gestational age in sheep to provide essential information for effective flock management practices. The aims of this study were to evaluate the efficacy and practical aspects for using two ultrasound techniques, transabdominal and transrectal, in determining the gestational age in Wurttemberg ewes. Monitoring of embryo and fetus developmental stages during the ewes' gestation was carried out with real time ultrasound using a transabdominal convex probe, frequency 3.5 MHz, and a transrectal linear probe, frequency 7.5 MHz. The size of the embryonic vesicle during the period from the 23rd to the 38th day of gestation can be used as a confirmational indicator of gestational age when the transrectal probe is used. The occipital nasal diameter correlated with the gestational age with both transabdominal (P < 0.05) and transrectal probes (P < 0.01) from the 46th to the 63rd day of gestation. The biparietal diameter of the fetal head measured by transabdominal probe during the period from the 46th to the 63rd day of gestation correlates with gestation age (P < 0.05). The diameter of the fetal eye orbit monitored by transrectal probe from the 46th to the 63rd day of gestation also correlated well with gestational age (P < 0.05). C1 [Petrujkic, Branko T.] Univ Belgrade, Fac Vet Med, Dept Nutr & Bot, Blvd Oslobodjenja 18, Belgrade 11000, Serbia. [Cojkic, Aleksandar] Univ Belgrade, Fac Vet Med, Blvd Oslobodjenja 18, Belgrade 11000, Serbia. [Dimitrijevic, Vladimir; Savic, Mila] Univ Belgrade, Fac Vet Med, Dept Anim Husb & Genet, Blvd Oslobodjenja 18, Belgrade 11000, Serbia. [Petrujkic, Katarina; Masulovic, Dragan] Ctr Radiol & Magnet Resonance, Clin Ctr Serbia, Belgrade, Serbia. [Jeremic, Ivan] PKB Corp Belgrade, Padinska Skela, Belgrade, Serbia. [Pesic, Miodrag] Vet Specialists Inst Nis, Nish, Serbia. [Beier, Ross C.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX USA. RP Petrujkic, BT (reprint author), Univ Belgrade, Fac Vet Med, Dept Nutr & Bot, Blvd Oslobodjenja 18, Belgrade 11000, Serbia. EM petrujkic@vet.bg.ac.rs OI Petrujkic, Branko/0000-0002-3112-4589 FU Ministry of Education, Science and Technological Development of the Republic of Serbia; Kingdom of Norway, HERD Project [09/1548, 332 160UAngstrom] FX This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia as well as by the Kingdom of Norway, HERD Project, 2010-2015; Project no.: 09/1548; 332 160U angstrom. NR 14 TC 0 Z9 0 U1 1 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1344-3941 EI 1740-0929 J9 ANIM SCI J JI Anim. Sci. J. PD FEB PY 2016 VL 87 IS 2 BP 197 EP 201 DI 10.1111/asj.12421 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA DC3TC UT WOS:000369140700006 PM 26223772 ER PT J AU Deetz, K Klose, M Kirchner, I Cubasch, U AF Deetz, K. Klose, M. Kirchner, I. Cubasch, U. TI Numerical simulation of a dust event in northeastern Germany with a new dust emission scheme in COSMO-ART SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Mineral dust; Dust model; Regional atmospheric model; COSMO-ART; PM10 ID EROSION THRESHOLD; MINERAL DUST; TRANSPORT; MODEL; IMPACTS; AEROSOL; AREAS AB The dust emission scheme of Shao (2004) has been implemented into the regional atmospheric model COSMO-ART and has been applied to a severe dust event in northeastern Germany on 8th April 2011. The model sensitivity to soil moisture and vegetation cover has been studied. Soil moisture has been found to be relatively high in the model during the investigation period and has been reduced by different degree to investigate the resulting changes in dust emissions. Two different vegetation datasets have been tested as model input: the climatological vegetation cover data of COSMO-ART (ECOCLIMAP) and the SPOT5 remote sensing vegetation cover data for the time of the event. By varying soil moisture, vegetation cover and by restricting the potential emission area, a set of eleven simulations was generated. Vegetation cover during the event was about 24% lower on average compared to the climatological mean. Thus, dust emissions modeled with SPOTS vegetation exceeded that modeled with climatological data by a factor of about 5. The modeled dust concentrations were compared with in-situ measurements of aerosol concentration. The temporal evolutions of simulations and observations have significant correlations (0.42-0.85) especially in rural backgrounds. The lower correlations at urban sites are attributed to anthropogenic PM10 sources, which are not included in the model. However, a verification of the magnitude of modeled dust concentrations is not possible due to the uncertainty in soil moisture and emission area. (C) 2015 The Authors. Published by Elsevier Ltd. C1 [Deetz, K.; Kirchner, I.; Cubasch, U.] Free Univ Berlin, Inst Meteorol, Carl Heinrich Becker Weg 6-10, D-12165 Berlin, Germany. [Klose, M.] Univ Cologne, Inst Geophys & Meteorol, Pohligstr 3, D-50969 Cologne, Germany. [Deetz, K.] Karlsruhe Inst Technol, Inst Meteorol & Climate Res Troposphere Res IMK T, Hermann von Helmholtz 1, D-76344 Eggenstein Leopoldshafen, Germany. [Klose, M.] New Mexico State Univ, USDA ARS Jornada Expt Range, POB 30003,MSC3 JER, Las Cruces, NM 88003 USA. RP Deetz, K (reprint author), Free Univ Berlin, Inst Meteorol, Carl Heinrich Becker Weg 6-10, D-12165 Berlin, Germany.; Deetz, K (reprint author), Karlsruhe Inst Technol, Inst Meteorol & Climate Res Troposphere Res IMK T, Hermann von Helmholtz 1, D-76344 Eggenstein Leopoldshafen, Germany. EM konrad.deetz@kit.edu OI Klose, Martina/0000-0001-8190-3700 NR 44 TC 1 Z9 1 U1 1 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD FEB PY 2016 VL 126 BP 87 EP 97 DI 10.1016/j.atmosenv.2015.11.045 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DC1DY UT WOS:000368958200010 ER PT J AU Islam, MS Fang, DD Thyssen, GN Delhom, CD Liu, YL Kim, HJ AF Islam, Md S. Fang, David D. Thyssen, Gregory N. Delhom, Chris D. Liu, Yongliang Kim, Hee Jin TI Comparative fiber property and transcriptome analyses reveal key genes potentially related to high fiber strength in cotton (Gossypium hirsutum L.) line MD52ne SO BMC PLANT BIOLOGY LA English DT Article DE Bundle fiber strength; Cellulose assembly; Ethylene; Gossypium hirsutum; Individual fiber strength; Near-isogenic line; Receptor-like kinase ID FT-IR SPECTROSCOPY; SEED COAT MUCILAGE; CELL-WALL; EXPRESSION ANALYSIS; IMAGE-ANALYSIS; IM MUTANT; ARABIDOPSIS; ELONGATION; PROTEIN; PLANT AB Background: Individual fiber strength is an important quality attribute that greatly influences the strength of the yarn spun from cotton fibers. Fiber strength is usually measured from bundles of fibers due to the difficulty of reliably measuring strength from individual cotton fibers. However, bundle fiber strength (BFS) is not always correlated with yarn strength since it is affected by multiple fiber properties involved in fiber-to-fiber interactions within a bundle in addition to the individual fiber strength. Molecular mechanisms responsible for regulating individual fiber strength remain unknown. Gossypium hirsutum near isogenic lines (NILs), MD52ne and MD90ne showing variations in BFS provide an opportunity for dissecting the regulatory mechanisms involved in individual fiber strength. Results: Comprehensive fiber property analyses of the NILs revealed that the superior bundle strength of MD52ne fibers resulted from high individual fiber strength with minor contributions from greater fiber length. Comparative transcriptome analyses of the NILs showed that the superior bundle strength of MD52ne fibers was potentially related to two signaling pathways: one is ethylene and the interconnected phytohormonal pathways that are involved in cotton fiber elongation, and the other is receptor-like kinases (RLKs) signaling pathways that are involved in maintaining cell wall integrity. Multiple RLKs were differentially expressed in MD52ne fibers and localized in genomic regions encompassing the strength quantitative trait loci (QTLs). Several candidate genes involved in crystalline cellulose assembly were also up-regulated in MD52ne fibers while the secondary cell wall was produced. Conclusion: Comparative phenotypic and transcriptomic analyses revealed differential expressions of the genes involved in crystalline cellulose assembly, ethylene and RLKs signaling pathways between the MD52ne and MD90ne developing fibers. Ethylene and its phytohormonal network might promote the elongation of MD52ne fibers and indirectly contribute to the bundle strength by potentially improving fiber-to-fiber interactions. RLKs that were suggested to mediate a coordination of cell elongation and SCW biosynthesis in other plants might be candidate genes for regulating cotton fiber cell wall assembly and strength. C1 [Islam, Md S.; Fang, David D.; Kim, Hee Jin] USDA ARS, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, POB 19687, New Orleans, LA 70124 USA. [Thyssen, Gregory N.] USDA ARS, So Reg Res Ctr, Cotton Chem & Utilizat Res Unit, POB 19687, New Orleans, LA 70124 USA. [Delhom, Chris D.; Liu, Yongliang] USDA ARS, So Reg Res Ctr, Cotton Struct & Qual Res Unit, POB 19687, New Orleans, LA 70124 USA. RP Kim, HJ (reprint author), USDA ARS, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, POB 19687, New Orleans, LA 70124 USA. EM heejin.kim@ars.usda.gov FU USDA-ARS CRIS project [6054-21000-017-00D]; Cotton Incorporated grant [12-199] FX This project was supported by USDA-ARS CRIS project #6054-21000-017-00D and Cotton Incorporated grant #12-199. NR 79 TC 7 Z9 7 U1 4 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD FEB 1 PY 2016 VL 16 AR 36 DI 10.1186/s12870-016-0727-2 PG 19 WC Plant Sciences SC Plant Sciences GA DC2MY UT WOS:000369052000002 PM 26833213 ER PT J AU Agarwal, UP Ralph, SA Reiner, RS Baez, C AF Agarwal, Umesh P. Ralph, Sally A. Reiner, Richard S. Baez, Carlos TI Probing crystallinity of never-dried wood cellulose with Raman spectroscopy SO CELLULOSE LA English DT Article DE Plant cell wall; Cellulose structure; Crystallinity; Microfibril; Nanocellulose; Raman spectroscopy; X-ray diffraction ID PLANT-CELL WALLS; TEMPO-MEDIATED OXIDATION; C-13 NMR-SPECTROSCOPY; SOLID-STATE C-13-NMR; SUPRAMOLECULAR STRUCTURE; NATIVE CELLULOSES; I CRYSTALLINITY; ACID-HYDROLYSIS; PICEA-MARIANA; SPRUCE WOOD AB The structure of wood cell wall cellulose in its native state remains poorly understood, limiting the progress of research and development in numerous areas, including plant science, biofuels, and nanocellulose based materials. It is generally believed that cellulose in cell wall microfibrils has both crystalline and amorphous regions. However, there is evidence that appears to be contrary to this assumption. Here we show, using 1064-nm FT-Raman spectroscopy, that (1) compared to the crystalline state, cellulose in the never-dried native state is laterally aggregated but in a less-than crystalline state wherein internal chains are water-accessible, (2) hydroxymethyl groups (CH2OH) in cellulose exist not only in the tg conformation but also in the gt rotamer form, and (3) in native-state fibrils, low-frequency Raman bands due to cellulose crystal domains are absent, indicating the lack of crystallinity. Further evidence of the absence of crystallinity of the fibrils was the failure of the normal 64 % H2SO4 hydrolysis procedure to produce nanocellulose crystals from untreated wood. X-ray diffraction data obtained on wood, treated-wood, and wood-cellulose samples were consistent with the new finding and indicated that full-width-at-half-height of the X-ray diffractograms and lateral disorder in samples as measured by Raman were correlated (R-2 = 0.95). C1 [Agarwal, Umesh P.; Ralph, Sally A.; Reiner, Richard S.; Baez, Carlos] USDA FS, Fiber & Chem Sci Res, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. RP Agarwal, UP (reprint author), USDA FS, Fiber & Chem Sci Res, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM uagarwal@fs.fed.us FU NSF through the University of Wisconsin Materials Research Science and Engineering Center [DMR-1121288] FX The authors thank Dr. Akira Isogai (Tokyo University) for providing Tunicate and Cladophora celluloses. We acknowledge Fred Matt of the FPL Analytical Chemistry and Microscopy Laboratory Unit for carrying out the composition analyses of the samples. They extend their gratitude to Dr. J. Y. Zhu (FPL) for providing samples of untreated and SPORL-treated Douglas fir used in this work. The authors are grateful to Dr. Alfred French for the editorial review of the manuscript. The authors gratefully acknowledge use of X-ray facilities and instrumentation supported by NSF through the University of Wisconsin Materials Research Science and Engineering Center (DMR-1121288). NR 67 TC 9 Z9 9 U1 17 U2 51 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD FEB PY 2016 VL 23 IS 1 BP 125 EP 144 DI 10.1007/s10570-015-0788-7 PG 20 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DB8YI UT WOS:000368802700005 ER PT J AU Xiang, ZY Gao, WH Chen, LH Lan, W Zhu, JY Runge, T AF Xiang, Zhouyang Gao, Wenhua Chen, Liheng Lan, Wu Zhu, J. Y. Runge, Troy TI A comparison of cellulose nanofibrils produced from Cladophora glomerata algae and bleached eucalyptus pulp SO CELLULOSE LA English DT Article DE Bleached eucalyptus pulp; Cellulose nanofibrils; Cladophora glomerata; Enzymatic hydrolysis; Green algae; X-ray diffraction ID NEUTRON FIBER DIFFRACTION; HYDROGEN-BONDING SYSTEM; SYNCHROTRON X-RAY; CRYSTAL-STRUCTURE; GREEN-ALGAE; NANOCELLULOSE; HYDROLYSIS; COMPOSITE; FEATURES; SISAL AB Cladophora, a fresh-water green macroalgae, has unique cellulose properties and thus may be promising for production of cellulose nanofibrils (CNFs). Cellulose was extracted from Cladophora glomerata and subjected to microfluidization with or without enzymatic hydrolysis pretreatment to produce CNFs. Increasing microfluidization passes produced smaller algal CNFs with more uniform sizes and lower crystallinity. Combining microfluidization with an enzymatic hydrolysis pretreatment, the algal CNFs' crystallinity decreased further, but the diameter distribution showed little change. The algal CNFs were compared to CNFs produced from bleached eucalyptus pulp (BEP), with the algal CNFs having significantly higher crystallinity, smaller diameter and better thermal stability. The XRD spectra of algal and BEP CNFs were simulated by Mercury 3.0 software. The results indicated that BEP CNFs are mainly cellulose I beta with a random orientation, while algal CNFs are likely a hybrid of cellulose I alpha with preferred orientations along the [100] and [010] axes with a March-Dollase factor of 0.65. C1 [Xiang, Zhouyang; Gao, Wenhua; Chen, Liheng] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China. [Chen, Liheng; Zhu, J. Y.] USDA, Forest Prod Lab, Forest Serv, Madison, WI 53726 USA. [Lan, Wu; Zhu, J. Y.; Runge, Troy] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. RP Gao, WH (reprint author), S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China.; Runge, T (reprint author), Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. EM segaowenhua@scut.edu.cn; trunge@wisc.edu RI Chen, Liheng/E-1985-2014 OI Chen, Liheng/0000-0001-7501-7972 FU National Natural Science Foundation of China [31400512]; US Department of Agriculture NIFA, under Critical Agricultural Material Grant [2013-38202-20400] FX This work was supported by the National Natural Science Foundation of China (31400512) and US Department of Agriculture NIFA, under Critical Agricultural Material Grant (2013-38202-20400). The authors would like to give their appreciations to Dr. Alfred French for personal communications about the simulation of XRD patterns and to Dr. Renil Anthony for his assistance with algae collection. NR 38 TC 2 Z9 2 U1 13 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD FEB PY 2016 VL 23 IS 1 BP 493 EP 503 DI 10.1007/s10570-015-0840-7 PG 11 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DB8YI UT WOS:000368802700033 ER PT J AU Wilson, RS Hardisty, DJ Epanchin-Niell, RS Runge, MC Cottingham, KL Urban, DL Maguire, LA Hastings, A Mumby, PJ Peters, DPC AF Wilson, Robyn S. Hardisty, David J. Epanchin-Niell, Rebecca S. Runge, Michael C. Cottingham, Kathryn L. Urban, Dean L. Maguire, Lynn A. Hastings, Alan Mumby, Peter J. Peters, Debra P. C. TI A typology of time-scale mismatches and behavioral interventions to diagnose and solve conservation problems SO CONSERVATION BIOLOGY LA English DT Article DE decision theory; economics; psychology; socioecological systems; temporal lags ID CLIMATE-CHANGE; SCIENCE; SYSTEMS; CHOICE; RISK AB Ecological systems often operate on time scales significantly longer or shorter than the time scales typical of human decision making, which causes substantial difficulty for conservation and management in socioecological systems. For example, invasive species may move faster than humans can diagnose problems and initiate solutions, and climate systems may exhibit long-term inertia and short-term fluctuations that obscure learning about the efficacy of management efforts in many ecological systems. We adopted a management-decision framework that distinguishes decision makers within public institutions from individual actors within the social system, calls attention to the ways socioecological systems respond to decision makers' actions, and notes institutional learning that accrues from observing these responses. We used this framework, along with insights from bedeviling conservation problems, to create a typology that identifies problematic time-scale mismatches occurring between individual decision makers in public institutions and between individual actors in the social or ecological system. We also considered solutions that involve modifying human perception and behavior at the individual level as a means of resolving these problematic mismatches. The potential solutions are derived from the behavioral economics and psychology literature on temporal challenges in decision making, such as the human tendency to discount future outcomes at irrationally high rates. These solutions range from framing environmental decisions to enhance the salience of long-term consequences, to using structured decision processes that make time scales of actions and consequences more explicit, to structural solutions aimed at altering the consequences of short-sighted behavior to make it less appealing. Additional application of these tools and long-term evaluation measures that assess not just behavioral changes but also associated changes in ecological systems are needed. C1 [Wilson, Robyn S.] Ohio State Univ, Sch Environm & Nat Resources, 210 Kottman Hall,2021 Coffey Rd, Columbus, OH 43221 USA. [Hardisty, David J.] Univ British Columbia, Sauder Sch Business, 2053 Main Mall, Vancouver, BC V6T 1Z2, Canada. [Epanchin-Niell, Rebecca S.] Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA. [Runge, Michael C.] US Geol Survey, Patuxent Wildlife Res Ctr, 12100 Beech Forest Rd, Laurel, MD 20708 USA. [Cottingham, Kathryn L.] Dartmouth Coll, Dept Biol Sci, 78 Coll St, Hanover, NH 03755 USA. [Urban, Dean L.; Maguire, Lynn A.] Duke Univ, Nicholas Sch Environm, Box 90328, Durham, NC 27708 USA. [Hastings, Alan] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Mumby, Peter J.] ARC Ctr Excellence Reef Studies, Townsville, Qld 4811, Australia. [Mumby, Peter J.] Sch Biol Sci, Townsville, Qld 4811, Australia. [Peters, Debra P. C.] ARS, USDA, Jornada Expt Range, Las Cruces, NM 88003 USA. RP Wilson, RS (reprint author), Ohio State Univ, Sch Environm & Nat Resources, 210 Kottman Hall,2021 Coffey Rd, Columbus, OH 43221 USA. EM wilson.1376@osu.edu FU National Socio-Environmental Synthesis Center (SESYNC) from the National Science Foundation [DBI-1052875]; University of Maryland; University of Maryland Center for Environmental Science, and Resources; NSF Behavioral and Cognitive Sciences grant through the Dynamics of Coupled Natural and Human Systems Program [BCS-1114934]; UC AES project [CA-D-ESP-2163-H]; NSF [DEB-1009957] FX This work was supported by the National Socio-Environmental Synthesis Center (SESYNC) under funding received from the National Science Foundation DBI-1052875 with additional support from University of Maryland, University of Maryland Center for Environmental Science, and Resources for the Future. This work was also supported by an NSF Behavioral and Cognitive Sciences grant through the Dynamics of Coupled Natural and Human Systems Program (BCS-1114934), as well as UC AES project CA-D-ESP-2163-H and NSF grant DEB-1009957. We are grateful to F. Johnson and 2 anonymous reviewers for their thoughtful comments. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 49 TC 3 Z9 3 U1 12 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD FEB PY 2016 VL 30 IS 1 BP 42 EP 49 DI 10.1111/cobi.12632 PG 8 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DC0WG UT WOS:000368938000006 PM 26390368 ER PT J AU Ruffing, CM Dwire, KA Daniels, MD AF Ruffing, Claire M. Dwire, Kathleen A. Daniels, Melinda D. TI Carbon pools in stream-riparian corridors: legacy of disturbance along mountain streams of south-eastern Wyoming SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE carbon budget; in-stream carbon; riparian carbon; disturbance; bark beetle ID LARGE WOODY DEBRIS; ORGANIC-MATTER; UNITED-STATES; OLD-GROWTH; TERRESTRIAL ECOSYSTEMS; HEADWATER STREAMS; PINE-BEETLE; FORESTS; DYNAMICS; BIOMASS AB Streams and their accompanying riparian environments are intrinsic components of terrestrial carbon cycling. However, they have been understudied in terms of the magnitude of their storage components and the role of disturbance in determining carbon storage capacity. This study presents partial carbon budgets for stream-riparian corridors along six study reaches in mountain headwater streams of southeast Wyoming to evaluate the impact of tie-driving, a historic disturbance legacy, on contemporary carbon storage. Detailed measurements of biomass were collected for in-stream components of carbon including fine and coarse particulate organic matter and in-stream large wood. Biomass was also estimated for riparian components including standing trees (live and dead), regenerating conifers, shrubs and herbaceous vegetation, downed wood, litter, and duff (partially decayed litter). Biomass was converted to carbon for all components and differences in storage were compared between tie-driven and non-driven reaches. Carbon content in riparian soils (to approximately 20 cm) was also measured. Twice the amount of carbon was stored in the riparian areas relative to the streams; most carbon was stored in standing trees (live and dead). While overall carbon storage within the riparian areas and streams were similar between disturbance conditions, the amount of carbon stored in large in-stream wood and downed wood on the floodplain was significantly higher in systems that were not tie-driven. The results of this study indicate that legacies of tie-driving influence carbon storage within the region, while also capturing baseline estimates of carbon storage in the wake of recent bark beetle infestations. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Ruffing, Claire M.] Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Dwire, Kathleen A.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO USA. [Daniels, Melinda D.] Stroud Water Res Ctr, Avondale, PA USA. RP Ruffing, CM (reprint author), Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. EM cmruffing@alaska.edu NR 75 TC 2 Z9 2 U1 3 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD FEB PY 2016 VL 41 IS 2 BP 208 EP 223 DI 10.1002/esp.3830 PG 16 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DB9IU UT WOS:000368830400005 ER PT J AU Cox, SE Booth, DT Likins, JC AF Cox, Samuel E. Booth, D. Terrance Likins, John C. TI Headcut Erosion in Wyoming's Sweetwater Subbasin SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Riparian; Wetland; Monitoring; Structure-from-motion; Remote sensing ID WESTERN UNITED-STATES; SOIL-EROSION; RIPARIAN ECOSYSTEMS; AERIAL PHOTOGRAPHS; RETREAT; PHOTOGRAMMETRY; INFILTRATION; RESTORATION; VEGETATION; MIGRATION AB Increasing human population and intensive land use combined with a warming climate and chronically diminished snowpacks are putting more strain on water resources in the western United States. Properly functioning riparian systems slow runoff and store water, thus regulating extreme flows; however, riparian areas across the west are in a degraded condition with a majority of riparian systems not in proper functioning condition, and with widespread catastrophic erosion of water-storing peat and organic soils. Headcuts are the leading edge of catastrophic channel erosion. We used aerial imagery (1.4-3.3-cm pixel) to locate 163 headcuts in riparian areas in the Sweetwater subbasin of central Wyoming. We found 1-m-the generally available standard resolution for land management-and 30-cm pixel imagery to be inadequate for headcut identification. We also used Structure-from-Motion models built from ground-acquired imagery to model 18 headcuts from which we measured soil loss of 425-720 m(3). Normalized by channel length, this represents a loss of 1.1-1.8 m(3) m(-1) channel. Monitoring headcuts, either from ground or aerial imagery, provides an objective indicator of sustainable riparian land management and identifies priority disturbance-mitigation areas. Image-based headcut monitoring must use data on the order of 3.3 cm ground sample distance, or greater resolution, to effectively capture the information needed for accurate assessments of riparian conditions. C1 [Cox, Samuel E.] USDI Bur Land Management, 5353 Yellowstone Rd, Cheyenne, WY 82009 USA. [Booth, D. Terrance] USDA Agr Res Serv, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. [Likins, John C.] USDI Bur Land Management, 1335 Main St, Lander, WY 82520 USA. RP Cox, SE (reprint author), USDI Bur Land Management, 5353 Yellowstone Rd, Cheyenne, WY 82009 USA. EM secox@blm.gov NR 78 TC 0 Z9 0 U1 2 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD FEB PY 2016 VL 57 IS 2 BP 450 EP 462 DI 10.1007/s00267-015-0610-1 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DB6PZ UT WOS:000368638500015 PM 26410166 ER PT J AU Tolk, JA Evett, SR Xu, WW Schwartz, RC AF Tolk, Judy A. Evett, Steven R. Xu, Wenwei Schwartz, Robert C. TI Constraints on water use efficiency of drought tolerant maize grown in a semi-arid environment SO FIELD CROPS RESEARCH LA English DT Article DE Water use efficiency; Boundary analysis; Evapotranspiration; Yield potential ID GRAIN-YIELD; STRESS TOLERANCE; TEMPERATE MAIZE; HIGH-PLAINS; CORN-BELT; EVAPOTRANSPIRATION; PRODUCTIVITY; IMPROVEMENT; WHEAT; IRRIGATION AB Identifying the constraints on crop water use efficiency (WUE) will help develop strategies to mitigate these limitations, potentially guiding agronomic and irrigation management strategies as well as providing needed directions in breeding. Boundary functions identify the upper limits of yield per unit of crop water use, and are useful for identifying constraints on WUE. The objectives of this research were to (1) develop a boundary function for maize using data (n=260) from research projects conducted at Bushland, TX, and (2) compare the yields of two recently developed, drought tolerant maize (Zea mays L) hybrids with the,boundary function and examine possible abiotic and biotic constraints on maximizing water use efficiency. A commercially available hybrid and an experimental hybrid were grown in 2012 (high environmental stress environment) and 2013 (moderate stress environment) in 48 weighing lysimeters containing soil monoliths of either clay loam, silt loam, sandy loam, or fine sand in a rain shelter facility. Plant density was 8 plants m(-2); irrigation treatments were 50% and 80% replacement of predicted crop evapotranspiration. The high stress environment of large vapor deficits and high temperatures during early reproductive growth stages in 2012 compared with 2013 significantly constrained the yield potential of both hybrids. It also showed the difference in stress tolerance of the hybrids with significant differences in WUE, yield and yield components between hybrids. Soil textural class was a significant constraint on WUE and harvest index (HI), with larger WUE and HI from crops in the fine sand and sandy loam compared with those of the crops in the clay loam and silt loam. Continued increases in the tolerance of maize to abiotic and biotic stresses will be necessary to maintain maize production in the southern High Plains region as irrigation water resources decline. Published by Elsevier B.V. C1 [Tolk, Judy A.; Evett, Steven R.; Schwartz, Robert C.] ARS, USDA, PO Drawer 10, Bushland, TX 79012 USA. [Xu, Wenwei] Texas A&M Agrilife Res, 1102 East FM 1294, Lubbock, TX 79403 USA. RP Tolk, JA (reprint author), ARS, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM Judy.Tolk@ars.usda.gov NR 57 TC 1 Z9 1 U1 14 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 1 PY 2016 VL 186 BP 66 EP 77 DI 10.1016/j.fcr.2015.11.012 PG 12 WC Agronomy SC Agriculture GA DC4OL UT WOS:000369200100007 ER PT J AU Bunce, JA AF Bunce, James A. TI Responses of soybeans and wheat to elevated CO2 in free-air and open top chamber systems SO FIELD CROPS RESEARCH LA English DT Article DE Open top chamber; Free-air carbon dioxide enrichment; Soybean; Wheat; Photosynthesis; Stomatal conductance ID CARBON-DIOXIDE CONCENTRATION; STOMATAL CONDUCTANCE; ATMOSPHERIC CO2; SEED YIELD; PHOTOSYNTHESIS; GROWTH; FOOD; ENRICHMENT; CULTIVARS; DROUGHT AB With increasing demand for agricultural products, more confidence is needed concerning impacts of rising atmospheric CO2 on crop yields. Despite debate about the merits of free-air CO2 enrichment (FACE) and open top chamber (OTC) systems, there have been no reports comparing crop yield responses to elevated CO2 in FACE and OTC systems using the same cultivar and location. In this study soybeans and winter wheat were grown for two years in FACE and OTC systems at the same time and location. An elevated CO2 treatment of ambient plus similar to 200 mu mol mol(-1) was applied 24 h per day for one cultivar of each species in the first year, and two cultivars of each species in the second year. Leaf area index, and midday leaf gas exchange rates were measured periodically, and total above ground biomass and seed yield were determined at maturity. In soybean, seed yield was increased by elevated CO2 in both FACE and OTC in both cultivars and years. However, the ratio of seed yield at elevated CO2 to that at ambient CO2 averaged significantly higher in OTC (1.49) than in FACE (1.27). In wheat, grain yield was increased by 15-30% by elevated CO2 for both cultivars and years in the OTC, but was not increased in either cultivar or year in the FACE system. No differences in midday photosynthetic rates occurred between OTC and FACE in either species for either CO2 treatment, except one season in wheat, but stomatal conductance was more reduced by elevated CO2 in OTC than in FACE. Short-term temporal variation in CO2 concentration was larger in FACE than in OTC. It is not clear from these results which method produces plant responses equivalent to those which may occur with increased atmospheric CO2. Published by Elsevier B.V. C1 [Bunce, James A.] USDA ARS, Crop Syst & Global Change Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Bunce, JA (reprint author), USDA ARS, Crop Syst & Global Change Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM james.Bunce@ars.usda.gov NR 29 TC 2 Z9 2 U1 9 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 1 PY 2016 VL 186 BP 78 EP 85 DI 10.1016/j.fcr.2015.11.010 PG 8 WC Agronomy SC Agriculture GA DC4OL UT WOS:000369200100008 ER PT J AU Johnson, DW Trettin, CC Todd, DE AF Johnson, D. W. Trettin, C. C. Todd, D. E., Jr. TI Changes in forest floor and soil nutrients in a mixed oak forest 33 years after stem only and whole-tree harvest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Harvest; Clearcut; Long-term response; Soil response; Regeneration; Forest floor changes ID DECIDUOUS FOREST; NITROGEN; CALCIUM; PSEUDOREPLICATION; PRODUCTIVITY; MANAGEMENT; POTASSIUM AB Vegetation, forest floor, and soils were resampled at a mixed oak site in eastern Tennessee that had been subjected to stem only (SOH), whole-tree harvest (WTH), and no harvest (REF) 33 years previously. Although differences between harvest treatments were not statistically significant (P < 0.05), average diameter, height, basal area and biomass were 8-18% lower in the WTH than in the SOH treatment 33 years after harvest whereas they differed by 2% 15 years after harvest. In contrast to results 15 years post-harvest, total forest floor mass and nutrient contents were twofold greater in the WTH than in the SOH treatment at 33 years post-harvest, due largely to differences in Oa horizon mass. Soil total C concentrations increased significantly (P < 0.05) over the first 15 years post-harvest in both harvest treatments. Decreases in soil C between 15 and 33 years post-harvest were not statistically significant. Soil total N increased significantly in both harvest treatments over the first 15 years post-harvest. Consistent decreases in soil total N occurred in the WTH treatment between years 15 and 33 post-harvest that bordered on statistical significance whereas total N was stable over that time period in the SOH treatment. The increases and decreases in soil N content cannot be explained by any known processes of N inputs or outputs. Harvest treatment effects on both Ca2+ and me observed at 15 years post-harvest are still observable and significant at 33 years post-harvest, although decreases between 15 and 33 years were found. Treatment effects and changes in soil exchangeable Ca2+ and Mg2+ are consistent with known inputs from decomposing logging residues, inputs from atmospheric deposition, and increments in forest floor and vegetation. No treatment effects were found for soil extractable P, but steady decreases over time were found. No treatment or time effects were found for soil exchangeable K. (C) 2015 Elsevier B.V. All rights reserved. C1 [Johnson, D. W.; Todd, D. E., Jr.] Univ Nevada, Nat Resources & Environm Sci, Reno, NV 89557 USA. [Trettin, C. C.] USDA Forest Serv, 2730 Savannah Highway, Charleston, SC 29141 USA. RP Johnson, DW (reprint author), Univ Nevada, Nat Resources & Environm Sci, Reno, NV 89557 USA. EM dwj@cabnr.unr.edu FU US Forest Service, Southeast Forest Experiment Station FX Funding for this research was supplied by the US Forest Service, Southeast Forest Experiment Station. Technical help in collecting and analyzing samples by Julie Arnold is gratefully appreciated. Assistance in collecting samples by Greg Byrd, Bailie Todd, and Andrew Todd is greatly appreciated. NR 29 TC 2 Z9 4 U1 8 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2016 VL 361 BP 56 EP 68 DI 10.1016/j.foreco.2015.11.012 PG 13 WC Forestry SC Forestry GA DB8FZ UT WOS:000368753900006 ER PT J AU Bates, JD Davies, KW AF Bates, Jonathan D. Davies, Kirk W. TI Seasonal burning of juniper woodlands and spatial recovery of herbaceous vegetation SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Cheatgrass; Prescribed fire; Fuel reduction; Resilience; Resistance; Sagebrush ID PINUS-PONDEROSA FORESTS; WESTERN JUNIPER; GREAT-BASIN; PLANT-COMMUNITIES; NORTHERN ARIZONA; BROMUS-TECTORUM; RESTORATION TREATMENTS; UNDERSTORY VEGETATION; MANAGEMENT TREATMENTS; TRANSITION MODELS AB Decreased fire activity has been recognized as a main cause of expansion and infilling of North American woodlands. Pinon-juniper (Pinus-Juniperus) woodlands in the western United States have expanded in area 2-10-fold since the late 1800s. Woodland control measures using chainsaws, heavy equipment and prescribed fire are used to restore big sagebrush (Artemisia tridentate Nutt.) steppe plant communities and reduce woody fuel loading. Immediate objectives in the initial control of Pinon-juniper are; (1) recovery of perennial herbaceous species to restore site composition, structure and processes (resilience) and resist invasion and dominance by invasive annual grasses (resistance) and (2) reducing woody fuel accumulations. Spanning a 7 year period (2006-2012), we compared herbaceous recovery following cutting and prescribed fire on three sites in mid and late succession western juniper (Juniperus occidentalis spp. occidentalis Hook.) woodlands in southeast Oregon. Treatments were untreated controls, partial cutting followed by fall broadcast burning (SEP), clear-cut and leave (CUT), and clear-cut and burn in winter (JAN), and spring (APR). Cover of herbaceous species was measured in three zones; interspace, litter mats around tree stumps (stump), and beneath felled trees. In interspace zones of all treatments, comprising between 51% and 63% of site areas, perennial bunchgrasses dominated two sites and co-dominated with invasive annual grasses at one site after treatment. Burning in the JAN treatments, when fuel moisture and relative humidity were high and temperatures cooler, reduced disturbance severity in stump and felled tree zones, which maintained perennial herbaceous understories and prevented or limited the presence of invasive annuals. Burning felled juniper in SEP and APR treatments resulted in moderate to high fire severity in stump and felled tree zones. At two sites, these fires consumed all fuel up to the 1000-h fuel class, largely eliminated herbaceous perennials, and created islands within treatments that enhanced annual grass invasion and dominance. To maintain or boost site resilience and resistance following control of late successional woodlands, reducing Pinon-juniper fuels by burning in winter provides managers with a low-impact option for conserving sagebrush steppe. Published by Elsevier B.V. C1 [Bates, Jonathan D.; Davies, Kirk W.] ARS, USDA, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. RP Bates, JD (reprint author), ARS, USDA, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. EM jon.bates@oregonstate.edu FU USDA-Agricultural Research Service; Oregon State Agricultural Experiment Station in Burns, Oregon; Oregon State Agricultural Experiment Station FX Many thanks to the F. Otley (Otley Brothers Ranch, Diamond, Oregon) for providing land for conducting a significant portion of the study. The fire crew was C. Poulsen, R. Sharp and J. Bates. We recognize the many summer range technicians who assisted in the field and laboratory over the course of the study; C. Archuleta, J. Davies, J. Ellis, K. Haile, A. Herdrich, J. Jackson, J. Louder, K. Mumm, R. O'Connor, E. O'Connor, J. Jackson, J. Price, K. Price, J. Pryse, K. Ralston, D. Randall, B. Smith, J. Svejcar, M. Zabala, T. Zaugg and D. Zvirdin. Many thanks to range technicians R. Sharp, C. Poulson, and G. Pokorney who assisted in the various stages of the study. We thank Dr. D.M. Bates and Dr. D. Ganskopp, and the anonymous reviewers for their comments on previous drafts of this manuscript. The research was supported by the USDA-Agricultural Research Service and Oregon State Agricultural Experiment Station in Burns, Oregon. The Eastern Oregon Agricultural Research Center is jointly funded by the USDA-Agricultural Research Service and Oregon State Agricultural Experiment Station. NR 59 TC 1 Z9 1 U1 5 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2016 VL 361 BP 117 EP 130 DI 10.1016/j.foreco.2015.10.045 PG 14 WC Forestry SC Forestry GA DB8FZ UT WOS:000368753900011 ER PT J AU Sweitzer, RA Furnas, BJ Barrett, RH Purcell, KL Thompson, CM AF Sweitzer, R. A. Furnas, B. J. Barrett, R. H. Purcell, K. L. Thompson, C. M. TI Landscape fuel reduction, forest fire, and biophysical linkages to local habitat use and local persistence of fishers (Pekania pennanti) in Sierra Nevada mixed-conifer forests SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fisher; Pekania pennanti; Wildfires; Logging; Fuel reduction; Mastication ID NORTHERN FLYING SQUIRRELS; YOSEMITE-NATIONAL-PARK; SMALL MAMMALS; MARTES-PENNANTI; UNITED-STATES; RIM FIRE; CALIFORNIA; POPULATION; ASSOCIATIONS; OCCUPANCY AB Fire suppression and logging have contributed to major changes in California's Sierra Nevada forests. Strategically placed landscape treatments (SPLATS) are being used to reduce density of trees, shrubs, and surface fuels to limit wildfire intensity and spread, but may negatively impact fishers (Pekania pennanti). We used camera traps to survey for fishers among 1-km(2) grid cells of forest habitat in the Sierra National Forest, California. We used single-season (n = 894 cells) and multi-season (n = 361 cells) occupancy modeling to evaluate responses of fishers to fuel reduction in the 5 years prior to camera surveys. We also assessed occupancy in relation to burn history, elevation, and an index of canopy cover. Camera traps detected fishers most often between 1380 m and 1970 m elevation, and fisher occupancy was maximized at 0.80 at 1640 m elevation. Probability of detection was higher after initial fisher detection, in habitats with high canopy cover, and when surveys were done in fall to spring. Fisher occupancy was positively linked to canopy cover, and trended lower among cells with higher levels of managed burning or forest fires within 25 years of surveys, and in cells where 5 years of cumulative disturbance from restorative fuel reduction was higher. Local persistence declined 24% in areas with more restorative fuel reduction (0-100% of a cell), but was not diminished by prior burning, or disturbance from extractive activities (tree removals for commerce or hazard mitigation). Reduced local persistence by fishers in areas with extensive restorative fuel reduction was likely temporary; evidence from other sources intimated that they would resume higher use within a few years of ecological recovery. The trend for lower occupancy in extensively burned forest cells suggested that forest fires reduced but did not eliminate foraging opportunities for fishers. We also found that wildfires increased in frequency in our study area after the 1980s, and recent fires may increasingly impinge on higher elevation forests with higher fisher occupancy. Forest fuel reduction likely imposes a more limited short term cost to fisher habitat use than previously believed, but less is known about the responses of resting or denning fishers to management disturbance. Fuel reduction treatments could be intensified below 1450 m elevation, which may reduce spread of fires into higher elevation forests where fishers are more common, and where denning is focused. (C) 2015 Elsevier B.V. All rights reserved. C1 [Sweitzer, R. A.] Great Basin Inst, 16750 Mt Rose Highway, Reno, NV 89511 USA. [Furnas, B. J.] Calif Dept Fish & Wildlife, 601 Locust St, Redding, CA 96001 USA. [Barrett, R. H.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Purcell, K. L.; Thompson, C. M.] USDA Forest Serv, Pacific Southwest Res Stn, 2081 E Sierra Ave, Fresno, CA 93710 USA. RP Sweitzer, RA (reprint author), Great Basin Inst, 16750 Mt Rose Highway, Reno, NV 89511 USA. EM rsweitzer@thegreatbasininstitute.org; Brett.Furnas@wildlife.ca.gov; rbarrett@berkeley.edu; kpurcell@fs.fed.us; cthompson@fs.fed.us RI Purcell, Kathryn/S-2592-2016 FU USDA Forest Service Region 5; University of California Agricultural Experiment Station, United States FX The field effort would not have been possible without help from a dedicated team of staff and volunteers including J. Ashling, S. Bassing, A. Beaudette J. Busiek, A. Cellar, T. Day, Z. Eads, T. Gorman, D. Hardeman, D. Jackson, W. Mitchell, C. O'Brien, M. Ratchford, J. Ruthven, J. Schneiderman, W. Sicard, T. Thein, S. Vogel, R. Wise, T. Watson, and others. A. Amacher aided with camera surveys within Yosemite NP in 2009, H. Taylor contributed important insights on the FACTS geospatial database, and D. Smith provided information on historic and current silviculture in the Sierra NF. Local support was facilitated by B. Persson, A. Otto, and A. Lombardo. Two anonymous reviews provided comments and suggestions that significantly improved the manuscript. This study was part of the Sierra Nevada Adaptive Management Project (SNAMP), a joint effort between USDA Forest Service Region 5, the University of California, USDA Forest Service Pacific Southwest Research Station, US Fish and Wildlife Service, California Department of Water Resources, California Department of Fish and Wildlife, California Department of Forestry and Fire Protection, the University of Wisconsin Madison, and the University of Minnesota. USDA Forest Service Region 5 funded the majority of the field research, and the University of California Agricultural Experiment Station, United States funded the remainder. This is publication no. 44 from the SNAMP. NR 81 TC 5 Z9 5 U1 17 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2016 VL 361 BP 208 EP 225 DI 10.1016/j.foreco.2015.11.026 PG 18 WC Forestry SC Forestry GA DB8FZ UT WOS:000368753900019 ER PT J AU Petrie, MD Wildeman, AM Bradford, JB Hubbard, RM Lauenroth, WK AF Petrie, M. D. Wildeman, A. M. Bradford, J. B. Hubbard, R. M. Lauenroth, W. K. TI A review of precipitation and temperature control on seedling emergence and establishment for ponderosa and lodgepole pine forest regeneration SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Ponderosa pine; Lodgepole pine; Germination; Regeneration; Climate; Review ID WESTERN UNITED-STATES; CLIMATE-CHANGE IMPACTS; CONTORTA VAR LATIFOLIA; ROOT-GROWTH CAPACITY; CHANGE-TYPE DROUGHT; HIGH-SEVERITY FIRE; BRITISH-COLUMBIA; WHITE SPRUCE; CONIFER SEEDLINGS; ENGELMANN SPRUCE AB The persistence of ponderosa pine and lodgepole pine forests in the 21st century depends to a large extent on how seedling emergence and establishment are influenced by driving climate and environmental variables, which largely govern forest regeneration. We surveyed the literature, and identified 96 publications that reported data on dependent variables of seedling emergence and/or establishment and one or more independent variables of air temperature, soil temperature, precipitation and moisture availability. Our review suggests that seedling emergence and establishment for both species is highest at intermediate temperatures (20 to 25 degrees C), and higher precipitation and higher moisture availability support a higher percentage of seedling emergence and establishment at daily, monthly and annual timescales. We found that ponderosa pine seedlings may be more sensitive to temperature fluctuations whereas lodge pole pine seedlings may be more sensitive to moisture fluctuations. In a changing climate, increasing temperatures and declining moisture availability may hinder forest persistence by limiting seedling processes. Yet, only 23 studies in our review investigated the effects of driving climate and environmental variables directly. Furthermore, 74 studies occurred in a laboratory or greenhouse, which do not often replicate the conditions experienced by tree seedlings in a field setting. It is therefore difficult to provide strong conclusions on how sensitive emergence and establishment in ponderosa and lodgepole pine are to these specific driving variables, or to investigate their potential aggregate effects. Thus, the effects of many driving variables on seedling processes remain largely inconclusive. Our review stresses the need for additional field and laboratory studies to better elucidate the effects of driving climate and environmental variables on seedling emergence and establishment for ponderosa and lodgepole pine. (C) 2015 Elsevier B.V. All rights reserved. C1 [Petrie, M. D.; Wildeman, A. M.; Bradford, J. B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Petrie, M. D.; Lauenroth, W. K.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Hubbard, R. M.] USDA Forest Serv, Ft Collins, CO USA. RP Petrie, MD (reprint author), US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. EM mpetrie@usgs.gov RI Bradford, John/E-5545-2011 FU USDA Forest Service, Western Wildland Environmental Threat Assessment Center FX Portions of this study were funded by a grant from the USDA Forest Service, Western Wildland Environmental Threat Assessment Center. We would like to thank Laura Bojanowski for her help in conducting the literature review. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 139 TC 4 Z9 4 U1 9 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2016 VL 361 BP 328 EP 338 DI 10.1016/j.foreco.2015.11.028 PG 11 WC Forestry SC Forestry GA DB8FZ UT WOS:000368753900029 ER PT J AU Richardson, KL Hellier, BC Sinha, K AF Richardson, Kelley L. Hellier, Barbara C. Sinha, Kapil TI Characterization of wild Beta populations in and adjacent to sugar beet fields in the Imperial Valley, California SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Beta; Characterization; Genetic marker; Morphology; Population structure; Sugar beet; Taxonomy; Wild relative ID GENUS BETA; GENETIC-RELATIONSHIPS; WEED BEET; RELATIVES; MARKER; FLOW AB Populations of wild Beta L. species exist as weeds in commercial sugar beet (Beta vulgaris L. subspecies vulgaris) fields in the Imperial Valley, California. Significant losses to sugar yield and quality result if these wild plants are not removed. In cases of extreme infestation, fields are abandoned without harvest. No selective chemicals are available to differentiate conventional sugar beet from wild relatives and hand removal is labor intensive and expensive. Planting sugar beet varieties with tolerance to glyphosate is a potential solution for infested fields, but risk of gene flow to adjacent wild relatives must be determined. Previous research identified these populations as either Beta vulgaris L. subspecies maritima (L.) Arcang. or Beta macrocarpa Guss. This distinction is critical because B. v. subsp. maritima will readily cross hybridize with cultivated sugar beet while B. macrocarpa rarely will. In April 2011, we collected herbarium samples, mature seed, and leaf tissue from wild Beta populations in 25 infested sugar beet fields throughout the Imperial Valley. Bolting cultivated beets were identified at two locations. Taxonomy of whole plant herbarium samples was unclear due to wild beet stem elongation when under competition with sugar beet plants for canopy light. Morphology of plants from collected seed grown in non-competitive conditions assigned taxonomy of these populations to B. macrocarpa. We used molecular tools to determine the genetic structure of wild Beta populations throughout the Imperial Valley. Extracted DNA was genotyped with 22 simple sequence repeat molecular markers and evaluated for population structure. The bolting beet samples were clearly separated from the majority of B. macrocarpa samples, except for two. The remaining wild populations were further divided into two subgroups suggesting exchange of genetic information or a common ancestor. C1 [Richardson, Kelley L.; Sinha, Kapil] USDA ARS, 1636 East Alisal St, Salinas, CA 93905 USA. [Hellier, Barbara C.] Washington State Univ, USDA ARS, 59 Johnson Hall, Pullman, WA 99164 USA. RP Richardson, KL (reprint author), USDA ARS, 1636 East Alisal St, Salinas, CA 93905 USA. EM kelley.richardson@ars.usda.gov FU California Beet Growers Association FX The 2011 collecting trip was funded by the California Beet Growers Association. NR 19 TC 0 Z9 0 U1 4 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD FEB PY 2016 VL 63 IS 2 BP 305 EP 314 DI 10.1007/s10722-015-0250-6 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DB7WB UT WOS:000368726400012 ER PT J AU Nelson, SO Trabelsi, S AF Nelson, Stuart O. Trabelsi, Samir TI Historical Development of Grain Moisture Measurement and Other Food Quality Sensing Through Electrical Properties SO IEEE INSTRUMENTATION & MEASUREMENT MAGAZINE LA English DT Article ID DIELECTRIC-PROPERTIES; CEREAL-GRAINS; PERMITTIVITY; RF C1 [Nelson, Stuart O.; Trabelsi, Samir] USDA ARS, Athens, GA 30613 USA. RP Nelson, SO; Trabelsi, S (reprint author), USDA ARS, Athens, GA 30613 USA. EM Stuart.Nelson@ARS.USDA.GOV; Samir.Trabelsi@ARS.USDA.GOV NR 17 TC 0 Z9 0 U1 6 U2 8 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1094-6969 EI 1941-0123 J9 IEEE INSTRU MEAS MAG JI IEEE Instrum. Meas. Mag. PD FEB PY 2016 VL 19 IS 1 BP 16 EP 23 PG 8 WC Engineering, Electrical & Electronic; Instruments & Instrumentation SC Engineering; Instruments & Instrumentation GA DC1ME UT WOS:000368979600005 ER PT J AU Trabelsi, S Nelson, SO AF Trabelsi, Samir Nelson, Stuart O. TI Microwave Sensing of Quality Attributes of Agricultural and Food Products SO IEEE INSTRUMENTATION & MEASUREMENT MAGAZINE LA English DT Article ID MOISTURE-CONTENT; BULK-DENSITY; PARTICULATE MATERIALS; DIELECTRIC-PROPERTIES; GRANULAR-MATERIALS; GRAIN C1 [Trabelsi, Samir; Nelson, Stuart O.] USDA ARS, Athens, GA 30613 USA. RP Trabelsi, S; Nelson, SO (reprint author), USDA ARS, Athens, GA 30613 USA. EM Samir.Trabelsi@ARS.USDA.GOV; Stuart.Nelson@ARS.USDA.GOV NR 14 TC 1 Z9 1 U1 2 U2 5 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1094-6969 EI 1941-0123 J9 IEEE INSTRU MEAS MAG JI IEEE Instrum. Meas. Mag. PD FEB PY 2016 VL 19 IS 1 BP 36 EP 41 PG 6 WC Engineering, Electrical & Electronic; Instruments & Instrumentation SC Engineering; Instruments & Instrumentation GA DC1ME UT WOS:000368979600009 ER PT J AU Zhang, XB Xu, MG Liu, J Sun, N Wang, BR Wu, LH AF Zhang, Xubo Xu, Minggang Liu, Jian Sun, Nan Wang, Boren Wu, Lianhai TI Greenhouse gas emissions and stocks of soil carbon and nitrogen from a 20-year fertilised wheat -maize intercropping system: A model approach SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Soil organic matter; Greenhouse gas; Fertilisation; Crop yield; SPACSYS ID UPLAND RED SOIL; ORGANIC-CARBON; OXIDE EMISSIONS; SOUTHERN CHINA; AGRICULTURAL SOILS; SAMPLING FREQUENCY; CROPPING SYSTEMS; MINERAL NITROGEN; N2O EMISSIONS; SEQUESTRATION AB Accurate modelling of agricultural management impacts on greenhouse gas emissions and the cycling of carbon and nitrogen is complicated due to interactions between various processes and the disturbance caused by field management. In this study, a process-based model, the Soil-Plant-Atmosphere Continuum System (SPACSYS), was used to simulate the effects of different fertilisation regimes on crop yields, the dynamics of soil organic carbon (SOC) and total nitrogen (SN) stocks from 1990 to 2010, and soil CO2 (2007-2010) and N2O (2007-2008) emissions based on a long-term fertilisation experiment with a winter-wheat (Triticum Aestivum L.) and summer-maize (Zea mays L.) intercropping system in Eutric Cambisol (FAO) soil in southern China. Three fertilisation treatments were 1) unfertilised (Control), 2) chemical nitrogen, phosphorus and potassium (NPK), and 3) NPK plus pig manure (NPKM). Statistical analyses indicated that the SPACSYS model can reasonably simulate the yields of wheat and maize, the evolution of SOC and SN stocks and soil CO2 and N2O emissions. The simulations showed that the NPKM treatment had the highest values of crop yields, SOC and SN stocks, and soil CO2 and N2O emissions were the lowest from the Control treatment. Furthermore, the simulated results showed that manure amendment along with chemical fertiliser applications led to both C (1017 +/- 470 kg C ha(-1) yr(-1)) and N gains (91.7 +/- 15.1 kg N ha(-1) yr(-1)) in the plant-soil system, while the Control treatment caused a slight loss in C and N. In conclusion, the SPACSYS model can accurately simulate the processes of C and N as affected by various fertilisation treatments in the red soil. Furthermore, application of chemical fertilisers plus manure could be a suitable management for ensuring crop yield and sustaining soil fertility in the red soil region, but the ratio of chemical fertilisers to manure should be optimized to reduce C and N losses to the environment. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Zhang, Xubo; Xu, Minggang; Sun, Nan; Wang, Boren] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Natl Engn Lab Improving Qual Arable Land, Beijing 100081, Peoples R China. [Zhang, Xubo; Wu, Lianhai] Rothamsted Res, Sustainable Soils & Grassland Syst Dept, Okehampton EX20 2SB, Devon, England. [Liu, Jian] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Xu, MG (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Natl Engn Lab Improving Qual Arable Land, Beijing 100081, Peoples R China. EM xuminggang@caas.cn RI Wu, Lianhai/B-1892-2016; Liu, Jian/P-4050-2014 OI Liu, Jian/0000-0003-4199-1296 FU National Natural Science Foundation of China [41171239]; National Basic Research Program of China [2011CB100501]; Biotechnology and Biological Sciences Research Council (BBSRC); China Scholarship Council [201303250075] FX Financial support was from the National Natural Science Foundation of China (41171239) and the National Basic Research Program of China (2011CB100501). Rothamsted Research receives strategic funding support from the Biotechnology and Biological Sciences Research Council (BBSRC). X. Zhang received funds from China Scholarship Council (No. 201303250075). The authors thank Dr T. H. Misselbrook for his constructive comment and suggestions on the manuscript. The authors acknowledge the colleagues at the long-term fertilization experimental station at Qiyang, Hunan province, China for their unremitting assistance. NR 53 TC 2 Z9 2 U1 21 U2 80 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD FEB 1 PY 2016 VL 167 BP 105 EP 114 DI 10.1016/j.jenvman2015.11.014 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DB8CQ UT WOS:000368745000013 PM 26615226 ER PT J AU Bhattacharya, SS Kim, KH Das, S Uchimiya, M Jeon, BH Kwon, E Szulejko, JE AF Bhattacharya, Satya Sundar Kim, Ki-Hyun Das, Subhasish Uchimiya, Minori Jeon, Byong Hun Kwon, Eilhann Szulejko, Jan E. TI A review on the role of organic inputs in maintaining the soil carbon pool of the terrestrial ecosystem SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Review DE CO2; Soil pollution; Soil organic C; C storage; Land use; Carbon storage & capture ID WHEAT CROPPING SYSTEM; TERRA PRETA SANITATION; LAND-USE CHANGE; LONG-TERM; HUMIC SUBSTANCES; GREEN MANURE; CLIMATE-CHANGE; RICE-WHEAT; MICROBIAL BIOMASS; MEDITERRANEAN CLIMATE AB Among the numerous sources of greenhouse gases, emissions of CO2 are considerably affected by changes in the extent and type of land use, e.g., intensive agriculture, deforestation, urbanization, soil erosion, or wetland drainage. As a feasible option to control emissions from the terrestrial ecosystems, the scientific community has explored the possibility of enhancing soil carbon (C) storage capacity. Thus, restoration of damaged lands through conservation tillage, crop rotation, cover cropping, reforestation, sub-soiling of compacted lands, sustainable water management practices, and organic manuring are the major antidotes against attenuation of soil organic C (SOC) stocks. In this research, we focused on the effect of various man-made activities on soil biotic organics (e.g., green-, farm-yard manure, and composts) to understand how C fluxes from various sources contribute to the establishment of a new equilibrium in the terrestrial ecosystems. Although such inputs substitute a portion of chemical fertilizers, they all undergo activities that augment the rate and extent of decay to deplete the SOC bank. Here, we provide perspectives on the balancing factors that control the mineralization rate of organic matter. Our arguments are placed in the background of different land use types and their impacts on forests, agriculture, urbanization, soil erosion, and wetland destruction. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Bhattacharya, Satya Sundar; Das, Subhasish] Tezpur Univ, Dept Environm Sci, Tezpur 784028, Assam, India. [Kim, Ki-Hyun; Szulejko, Jan E.] Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 133791, South Korea. [Uchimiya, Minori] USDA ARS, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Jeon, Byong Hun] Hanyang Univ, Dept Nat Resources & Environm Engn, Seoul 133791, South Korea. [Kwon, Eilhann] Sejong Univ, Environm & Energy Dept, Seoul 143747, South Korea. RP Kim, KH (reprint author), Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 133791, South Korea. EM kkim61@hanyang.ac.kr OI Jeon, Byong-Hun/0000-0002-5478-765X FU National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology (MEST) [2009-0093848]; Rural Development Administration, Republic of Korea [PJ01052101]; National Research Foundation of Korea (NRF) Grant - Korean Government (MSIP) [2914RA1A004893] FX This study was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) (No. 2009-0093848). This work was also carried out with the support of the "Cooperative Research Program for Agriculture Science & Technology Development" (Project title: Study on model development to control odor from hogpens, Project No. PJ01052101) Rural Development Administration, Republic of Korea. The sixth author also acknowledges the support made by a National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. 2914RA1A004893). NR 194 TC 1 Z9 1 U1 45 U2 178 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD FEB 1 PY 2016 VL 167 BP 214 EP 227 DI 10.1016/j.jenvman.2015.09.042 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA DB8CQ UT WOS:000368745000026 PM 26686074 ER PT J AU Kuhnle, RA Wren, DG Langendoen, EJ AF Kuhnle, Roger A. Wren, Daniel G. Langendoen, Eddy J. TI Erosion of Sand from a Gravel Bed SO JOURNAL OF HYDRAULIC ENGINEERING LA English DT Article ID SEDIMENT TRANSPORT; MODEL DEVELOPMENT; ENTRAINMENT; SIMULATION; FRAMEWORK; RIVERS; CREEK; FLOW AB Cleaning of fine sediment out of gravel streambeds has become an important method to restore affected stream habitats. Introducing the increased flows needed to entrain fine sediments without eroding the coarser fractions of the bed and potentially destroying its usefulness as a habitat requires careful selection of flow strength. Toward this end, a series of flume experiments was conducted on the entrainment of sand from immobile gravel beds to determine the depth to which the sand could be eroded without the gravel being mobilized. A series of steady flows was imposed on three gravel beds filled with sand with median sizes of 0.2, 0.3, and 0.9 mm. The shear stress of the series of flows ranged from just above the critical shear stress for initiation of motion of the sand up to 0.9 times the critical stress of the 36.1-mm gravel. The experiments continued until the rate of fine sediment exiting the channel was very small. It was found that the cleanout depth of the sand was reasonably predicted by estimating the shear stress in the gravel as the product of bed shear stress and the value of the cumulative probability distribution function of the elevations of the gravel surface evaluated at the surface of the sand layer. The appropriate initiation of sediment motion criterion was found to be a function of the shear velocity of the flow and the fall velocity of the fine grains. (C) 2015 American Society of Civil Engineers. C1 [Kuhnle, Roger A.; Wren, Daniel G.; Langendoen, Eddy J.] ARS, Natl Sedimentat Lab, USDA, 598 McElroy Dr, Oxford, MS 38655 USA. RP Kuhnle, RA (reprint author), ARS, Natl Sedimentat Lab, USDA, 598 McElroy Dr, Oxford, MS 38655 USA. EM roger.kuhnle@ars.usda.gov NR 38 TC 0 Z9 0 U1 8 U2 14 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9429 EI 1943-7900 J9 J HYDRAUL ENG JI J. Hydraul. Eng.-ASCE PD FEB PY 2016 VL 142 IS 2 AR 04015052 DI 10.1061/(ASCE)HY.1943-7900.0001071 PG 8 WC Engineering, Civil; Engineering, Mechanical; Water Resources SC Engineering; Water Resources GA DB9EZ UT WOS:000368820500008 ER PT J AU Chen, J Brissette, FP Zhang, XCJ AF Chen, Jie Brissette, Francois P. Zhang, Xunchang J. TI Hydrological Modeling Using a Multisite Stochastic Weather Generator SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Multisite weather generator (MulGETS); Hydrological modeling; Interstation correlation; Single-site weather generator (WeaGETS) ID CLIMATE-CHANGE IMPACT; DAILY PRECIPITATION; DAILY RAINFALL; SOIL-EROSION; LOESS PLATEAU; RIVER-BASIN; TEMPERATURE; SCENARIOS; VARIABILITY; SIMULATION AB Weather data are usually required at several locations over a large watershed, especially when using distributed models for hydrological simulations. In many applications, spatially correlated weather data can be provided by a multisite stochastic weather generator, which considers the spatial correlation of weather variables. Prior to using a multisite weather generator for hydrological modeling, its ability to adequately represent the proper hydrological response needs to be assessed. This study assesses the effectiveness of a new multisite weather generator (MulGETS) for hydrological modeling over a Canadian watershed in the Province of Quebec. Prior to hydrological modeling, MulGETS is first evaluated with respect to reproducing the spatial correlation and statistical characteristics of precipitation and temperature for the studied watershed. Hydrological simulations obtained from MulGETS-generated precipitation and temperature are then compared with those obtained from a single-site weather generator (WeaGETS) and a WeaGETS-based lumped approach (WeaGETS-lumped) that averages the climate series over all stations in a watershed before running the single-site weather generator. The hydrology is simulated using two hydrological models: the conceptually lumped model HSAMI and the physically based distributed model CEQUEAU. When using the conceptually lumped model, the weather time series is first averaged over all stations in the watershed. The results show that the monthly mean discharge is accurately represented by both MulGETS-generated and WeaGETS-lumped-generated precipitation and temperature, whereas it is considerably underestimated by WeaGETS data for the snowmelt period. The MulGETS and WeaGETS-lumped data also show significant advantages in representing the monthly streamflow variability, which is underestimated by the WeaGETS outputs. Additionally, MulGETS and WeaGETS-lumped consistently perform better than WeaGETS for simulating extreme flows (snowmelt high flows and summer-autumn high and low flows). However, no obvious difference in performance was found between MulGETS and WeaGETS-lumped data for hydrological modeling. Moreover, the use of a physically based distributed model with MulGETS did not result in any significant performance gain compared with the much simpler combination of WeaGETS-lumped with a lumped hydrological model for the studied watershed. Overall, this study indicates that a single-site weather generator combined with a lumped hydrological model is sufficient for accurate hydrological simulations, even in the case of a large watershed. (C) 2015 American Society of Civil Engineers. C1 [Chen, Jie; Brissette, Francois P.] Univ Quebec, Ecole Technol Super, Dept Construct Engn, 1100 Rue Notre Dame Ouest, Montreal, PQ H3C 1K3, Canada. [Zhang, Xunchang J.] USDA ARS, Grazinglands Res Lab, 7202 W Cheyenne St, El Reno, OK 73036 USA. RP Chen, J (reprint author), Univ Quebec, Ecole Technol Super, Dept Construct Engn, 1100 Rue Notre Dame Ouest, Montreal, PQ H3C 1K3, Canada. EM jie.chen@etsmtl.ca FU Natural Science and Engineering Research Council of Canada (NSERC); Hydro-Quebec; Ouranos Consortium on Regional Climatology and Adaption to Climate Change; Rio-Tinto-Alcan; Ontario Power Generation FX This work was partially supported by the Natural Science and Engineering Research Council of Canada (NSERC), Hydro-Quebec, the Ouranos Consortium on Regional Climatology and Adaption to Climate Change, Rio-Tinto-Alcan, and Ontario Power Generation. NR 76 TC 0 Z9 0 U1 2 U2 19 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD FEB PY 2016 VL 21 IS 2 DI 10.1061/(ASCE)HE.1943-5584.0001288 PG 11 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DB8ZN UT WOS:000368805800014 ER PT J AU Pinto, LC de Mello, CR Owens, PR Norton, LD Curi, N AF Pinto, Leandro Campos de Mello, Carlos Rogerio Owens, Phillip Ray Norton, Lloyd Darrell Curi, Nilton TI Role of Inceptisols in the Hydrology of Mountainous Catchments in Southeastern Brazil SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Atlantic forest; Hydropedology; Water recharge; Micromorphology; Streamflow; Mantiqueira range ID REFERENCE CROP EVAPOTRANSPIRATION; SOIL HYDRAULIC-PROPERTIES; LAND-USE; SOLAR-RADIATION; ORGANIC-MATTER; RAIN-FOREST; MANTIQUEIRA; TEMPERATURE AB Mantiqueira Range is the most important headwater region in southeastern Brazil, being responsible for streamflows that feed a significant part of Brazilian hydroelectric energy production. This region is extremely fragile with endemic species like those that compose the upper mountain cloud forest (UMCF), and the dominant soils are typical Inceptisols. These forest environments are rapidly disappearing in Brazil. The changing land use combined with shallow Inceptisols lead to important effects on hydrology. Therefore, studies addressing streamflow behavior in these landscapes are urgently needed for understanding the groundwater recharge process. The objectives of this study were to investigate the role of shallow Inceptisols linked to the land use changes in the streamflow behavior of the Mantiqueira Range region and to understand the mechanisms of water flow into the saturated zone. To validate the study, a comparative analysis was developed for a representative watershed of Mantiqueira Range, whose land use is divided into pasture and Atlantic Forest, known as Lavrinha Creek Watershed (LCW) and a typical UMCF located inside of LCW. The results showed that in general, UMCF has greater potential for water percolation beginning from the surface layer towards the saturated zone. Micromorphological soil images provided useful information about the soil pore system associated with the physical properties of Inceptisols, and this background helped to understand the water recharge process and streamflow behavior. In addition, the water balance for two years (2009-2011) in the UMCF showed that the flow stored in the saturated zone occurs rapidly, increasing its potential for storage, characterized by the process of refilling water in the Inceptisols soil profile under forests in this region. (C) 2015 American Society of Civil Engineers. C1 [Pinto, Leandro Campos; Curi, Nilton] Univ Fed Lavras, Dept Soil Sci, POB 3037, BR-37200000 Lavras, MG, Brazil. [de Mello, Carlos Rogerio] Univ Fed Lavras, Soil & Water Engn Grp, Dept Engn, POB 3037, BR-37200000 Lavras, MG, Brazil. [Owens, Phillip Ray] Purdue Univ, Dept Soil Sci, Lilly Hall Life Sci,915 W State St, W Lafayette, IN 47906 USA. [Owens, Phillip Ray] Purdue Univ, Dept Agron, Lilly Hall Life Sci,915 W State St, W Lafayette, IN 47906 USA. [Norton, Lloyd Darrell] Purdue Univ, Agron & Agr & Biol Engn, USDA ARS, Natl Soil Eros Res Lab, 275 South Russell St, W Lafayette, IN 47906 USA. RP Pinto, LC (reprint author), Univ Fed Lavras, Dept Soil Sci, POB 3037, BR-37200000 Lavras, MG, Brazil. EM leandcampos@yahoo.com.br RI de Mello, Carlos/G-5821-2012 OI de Mello, Carlos/0000-0002-6033-5342 FU FAPEMIG [588-CAG PPM-00132/14, PPM VIII-71-14]; CNPq; Capes FX The authors wish to thank FAPEMIG (588-CAG PPM-00132/14 and PPM VIII-71-14), CNPq and Capes for sponsoring this research. NR 49 TC 0 Z9 0 U1 1 U2 10 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD FEB PY 2016 VL 21 IS 2 AR 05015017 DI 10.1061/(ASCE)HE.1943-5584.0001275 PG 10 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DB8ZN UT WOS:000368805800006 ER PT J AU Scully, ED Gries, T Funnell-Harris, DL Xin, ZG Kovacs, FA Vermerris, W Sattler, SE AF Scully, Erin D. Gries, Tammy Funnell-Harris, Deanna L. Xin, Zhanguo Kovacs, Frank A. Vermerris, Wilfred Sattler, Scott E. TI Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Article DE Cinnamyl alcohol dehydrogenase (CAD); EMS mutagenesis; biofuels; C4 grass; forage ID CINNAMYL-ALCOHOL-DEHYDROGENASE; BICOLOR L. MOENCH; BROWN-MIDRIB MUTANTS; ACID O-METHYLTRANSFERASE; LACTATING DAIRY-COWS; BIOETHANOL PRODUCTION; BIOFUEL FEEDSTOCKS; FORAGE SORGHUM; GENE ENCODES; MAIZE AB The presence of lignin reduces the quality of lignocellulosic biomass for forage materials and feedstock for biofuels. In C4 grasses, the brown midrib phenotype has been linked to mutations to genes in the monolignol biosynthesis pathway. For example, the Bmr6 gene in sorghum (Sorghum bicolor) has been previously shown to encode cinnamyl alcohol dehydrogenase (CAD), which catalyzes the final step of the monolignol biosynthesis pathway. Mutations in this gene have been shown to reduce the abundance of lignin, enhance digestibility, and improve saccharification efficiencies and ethanol yields. Nine sorghum lines harboring five different bmr6 alleles were identified in an EMS-mutagenized TILLING population. DNA sequencing of Bmr6 revealed that the majority of the mutations impacted evolutionarily conserved amino acids while three-dimensional structural modeling predicted that all of these alleles interfered with the enzyme's ability to bind with its NADPH cofactor. All of the new alleles reduced in vitro CAD activity levels and enhanced glucose yields following saccharification. Further, many of these lines were associated with higher reductions in acid detergent lignin compared to lines harboring the previously characterized bmr6-ref allele. These bmr6 lines represent new breeding tools for manipulating biomass composition to enhance forage and feedstock quality. C1 [Scully, Erin D.; Gries, Tammy; Funnell-Harris, Deanna L.; Sattler, Scott E.] USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Scully, Erin D.; Sattler, Scott E.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Funnell-Harris, Deanna L.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Xin, Zhanguo] USDA ARS, Plant Stress & Germplasm Dev Unit, Lubbock, TX 79414 USA. [Kovacs, Frank A.] Univ Nebraska, Dept Chem, Kearney, NE 68849 USA. [Vermerris, Wilfred] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32610 USA. [Vermerris, Wilfred] Univ Florida, UF Genet Inst, Gainesville, FL 32610 USA. RP Sattler, SE (reprint author), USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA.; Sattler, SE (reprint author), Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. EM Scott.Sattler@ars.usda.gov FU Office of Science (BER), U.S. Department of Energy [DE-FG02-07ER64458]; USDA-ARS; CRIS [5440-21220-032-00D]; USDA AFRI [2011-67009-30026]; USDA Biomass Research and Development Initiative [2011-10006-30358]; U.S. DOE's International Affairs from the U.S. DOE's Office of Energy Efficiency and Renewable Energy, Bioenergy Technologies Office [DE-PI0000031] FX We thank John Toy, Alejandra Abril, and Yuk Kwan Tsui for technical assistance and Pat O'Neill for statistical support. This research was supported by the Office of Science (BER), U.S. Department of Energy grant DE-FG02-07ER64458 (Wilfred Vermerris and Scott E. Sattler), and additional funding from USDA-ARS, CRIS project 5440-21220-032-00D (S. E. S, Deanna L. Funnell-Harris.), USDA AFRI grant number 2011-67009-30026 (S. E. S, D. L. F. H.), USDA Biomass Research and Development Initiative grant number 2011-10006-30358 (W. V.), and the U.S. DOE's International Affairs under award number DE-PI0000031 from the U.S. DOE's Office of Energy Efficiency and Renewable Energy, Bioenergy Technologies Office (W.V). NR 55 TC 5 Z9 5 U1 7 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD FEB PY 2016 VL 58 IS 2 BP 136 EP 149 DI 10.1111/jipb.12375 PG 14 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DC1QS UT WOS:000368992100004 PM 26172142 ER PT J AU Segundo, FDS Medina, GN Ramirez-Medina, E Velazquez-Salinas, L Koster, M Grubman, MJ de los Santos, T AF Segundo, Fayna Diaz-San Medina, Gisselle N. Ramirez-Medina, Elizabeth Velazquez-Salinas, Lauro Koster, Marla Grubman, Marvin J. de los Santos, Teresa TI Synonymous Deoptimization of Foot-and-Mouth Disease Virus Causes Attenuation In Vivo while Inducing a Strong Neutralizing Antibody Response SO JOURNAL OF VIROLOGY LA English DT Article ID CODON-PAIR BIAS; RNA VIRUSES; DINUCLEOTIDE FREQUENCIES; VIRAL REPLICATION; CELL-CULTURE; RIG-I; LIVE; VACCINE; PROTEIN; MICE AB Codon bias deoptimization has been previously used to successfully attenuate human pathogens, including poliovirus, respiratory syncytial virus, and influenza virus. We have applied a similar technology to deoptimize the capsid-coding region (P1) of foot-and-mouth disease virus (FMDV). Despite the introduction of 489 nucleotide changes (19%), synonymous deoptimization of the P1 region rendered a viable FMDV progeny. The resulting strain was stable and reached cell culture titers similar to those obtained for wild-type (WT) virus, but at reduced specific infectivity. Studies in mice showed that 100% of animals inoculated with the FMDV A12 P1 deoptimized mutant (A12-P1 deopt) survived, even when the animals were infected at doses 100 times higher than the dose required to cause death by WT virus. All mice inoculated with the A12-P1 deopt mutant developed a strong antibody response and were protected against subsequent lethal challenge with WT virus at 21 days postinoculation. Remarkably, the vaccine safety margin was at least 1,000-fold higher for A12-P1 deopt than for WT virus. Similar patterns of attenuation were observed in swine, in which animals inoculated with A12-P1 deopt virus did not develop clinical disease until doses reached 1,000 to 10,000 times the dose required to cause severe disease in 2 days with WT A12. Consistently, high levels of antibody titers were induced, even at the lowest dose tested. These results highlight the potential use of synonymous codon pair deoptimization as a strategy to safely attenuate FMDV and further develop live attenuated vaccine candidates to control such a feared livestock disease. C1 [Segundo, Fayna Diaz-San; Medina, Gisselle N.; Koster, Marla; Grubman, Marvin J.; de los Santos, Teresa] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Segundo, Fayna Diaz-San] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Ramirez-Medina, Elizabeth; Velazquez-Salinas, Lauro] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr, Res Participat Program, Oak Ridge, TN USA. RP de los Santos, T (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. EM teresa.delossantos@ars.usda.gov FU U.S Department of Agriculture FX U.S Department of Agriculture provided funding to Fayna Diaz-San Segundo, Gisselle N. Medina, Elizabeth Ramirez-Medina, Lauro Velazquez-Salinas, Marla Koster, Marvin J Grubman, and Teresa de los Santos. NR 53 TC 2 Z9 2 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD FEB PY 2016 VL 90 IS 3 BP 1298 EP 1310 DI 10.1128/JVI.02167-15 PG 13 WC Virology SC Virology GA DC3WW UT WOS:000369150800012 ER PT J AU DeBlasio, SL Chavez, JD Alexander, MM Ramsey, J Eng, JK Mahoney, J Gray, SM Bruce, JE Cilia, M AF DeBlasio, Stacy L. Chavez, Juan D. Alexander, Mariko M. Ramsey, John Eng, Jimmy K. Mahoney, Jaclyn Gray, Stewart M. Bruce, James E. Cilia, Michelle TI Visualization of Host-Polerovirus Interaction Topologies Using Protein Interaction Reporter Technology SO JOURNAL OF VIROLOGY LA English DT Article ID POTATO-LEAFROLL-VIRUS; YELLOW DWARF VIRUS; VIRAL COAT PROTEIN; OXYGEN-EVOLVING COMPLEX; TOBACCO-MOSAIC-VIRUS; MAJOR CAPSID PROTEIN; APHID TRANSMISSION; PHOTOSYSTEM-II; IN-VIVO; NICOTIANA-BENTHAMIANA AB Demonstrating direct interactions between host and virus proteins during infection is a major goal and challenge for the field of virology. Most protein interactions are not binary or easily amenable to structural determination. Using infectious preparations of a polerovirus (Potato leafroll virus [PLRV]) and protein interaction reporter (PIR), a revolutionary technology that couples a mass spectrometric-cleavable chemical cross-linker with high-resolution mass spectrometry, we provide the first report of a host-pathogen protein interaction network that includes data-derived, topological features for every cross-linked site that was identified. We show that PLRV virions have hot spots of protein interaction and multifunctional surface topologies, revealing how these plant viruses maximize their use of binding interfaces. Modeling data, guided by cross-linking constraints, suggest asymmetric packing of the major capsid protein in the virion, which supports previous epitope mapping studies. Protein interaction topologies are conserved with other species in the Luteoviridae and with unrelated viruses in the Herpesviridae and Adenoviridae. Functional analysis of three PLRV-interacting host proteins in planta using a reverse-genetics approach revealed a complex, molecular tug-of-war between host and virus. Structural mimicry and diversifying selection-hallmarks of host-pathogen interactions-were identified within host and viral binding interfaces predicted by our models. These results illuminate the functional diversity of the PLRV-host protein interaction network and demonstrate the usefulness of PIR technology for precision mapping of functional host-pathogen protein interaction topologies. C1 [DeBlasio, Stacy L.; Alexander, Mariko M.; Ramsey, John; Mahoney, Jaclyn; Cilia, Michelle] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [DeBlasio, Stacy L.; Gray, Stewart M.; Cilia, Michelle] USDA ARS, Ithaca, NY 14853 USA. [Chavez, Juan D.; Bruce, James E.] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Alexander, Mariko M.; Gray, Stewart M.; Cilia, Michelle] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Eng, Jimmy K.] Univ Washington, Proteom Resources, Seattle, WA 98195 USA. RP Cilia, M (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA.; Cilia, M (reprint author), USDA ARS, Ithaca, NY 14853 USA.; Cilia, M (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. EM mlc68@cornell.edu FU National Science Foundation (NSF) [1354309]; USDA \ National Institute of Food and Agriculture (NIFA) [80622200002120] FX National Science Foundation (NSF) provided funding to James E Bruce and Michelle L. Cilia under grant number 1354309. USDA vertical bar National Institute of Food and Agriculture (NIFA) provided funding to Stewart M Gray and Michelle L. Cilia under grant number 80622200002120. NR 87 TC 3 Z9 3 U1 4 U2 17 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD FEB PY 2016 VL 90 IS 4 BP 1973 EP 1987 DI 10.1128/JVI.01706-15 PG 15 WC Virology SC Virology GA DC3XL UT WOS:000369152700024 PM 26656710 ER PT J AU Shi, GJ Zhang, ZC Friesen, TL Bansal, U Cloutier, S Wicker, T Rasmussen, JB Faris, JD AF Shi, Gongjun Zhang, Zengcui Friesen, Timothy L. Bansal, Urmil Cloutier, Sylvie Wicker, Thomas Rasmussen, Jack B. Faris, Justin D. TI Marker development, saturation mapping, and high-resolution mapping of the Septoria nodorum blotch susceptibility gene Snn3-B1 in wheat SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Wheat; Necrotrophic pathogen; Disease resistance; Map-based cloning; Molecular marker; Host-selective toxin ID EFFECTOR-TRIGGERED SUSCEPTIBILITY; HOST-SELECTIVE TOXIN; STAGONOSPORA-NODORUM; SENSITIVITY GENES; TRITICUM-AESTIVUM; GENOMIC ANALYSIS; POLYPLOID WHEAT; RESISTANCE; DISEASE; MAP AB Septoria nodorum blotch (SNB), caused by Parastagonospora nodorum, is a severe foliar and glume disease on durum and common wheat. Pathogen-produced necrotrophic effectors (NEs) are the major determinants for SNB on leaves. One such NE is SnTox3, which evokes programmed cell death and leads to disease when recognized by the wheat Snn3-B1 gene. Here, we developed saturated genetic linkage maps of the Snn3-B1 region using two F-2 populations derived from the SnTox3-sensitive line Sumai 3 crossed with different SnTox3-insensitive lines. Markers were identified and/or developed from various resources including previously mapped simple sequence repeats, bin-mapped expressed sequence tags, single nucleotide polymorphisms, and whole genome survey sequences. Subsequent high-resolution mapping of the Snn3-B1 locus in 5600 gametes delineated the gene to a 1.5 cM interval. Analysis of micro-colinearity of the Snn3-B1 region indicated that it was highly disrupted compared to rice and Brachypodium distachyon. The screening of a collection of durum and common wheat cultivars with tightly linked markers indicated they are not diagnostic for the presence of Snn3-B1, but can be useful for marker-assisted selection if the SnTox3 reactions of lines are first determined. Finally, we developed an ethyl methanesulfonate-induced mutant population of Sumai 3 where the screening of 408 M-2 families led to the identification of 17 SnTox3-insensitive mutants. These mutants along with the markers and high-resolution map developed in this research provide a strong foundation for the map-based cloning of Snn3-B1, which will broaden our understanding of the wheat-P. nodorum system and plant-necrotrophic pathogen interactions in general. C1 [Shi, Gongjun; Rasmussen, Jack B.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [Zhang, Zengcui; Friesen, Timothy L.; Faris, Justin D.] USDA ARS, Cereal Crops Res Unit, USDA ARS NPA NCSL, Red River Valley Agr Res Ctr, 1605 Albrecht BLVD, Fargo, ND 58102 USA. [Bansal, Urmil] Univ Sydney PBI Cobbity, Private Bag 4011, Narellan, NSW 2567, Australia. [Cloutier, Sylvie] Agr & Agri Food Canada, Eastern Cereal & Oil Res Ctr, KW Neatby Bldg,960 Carling Ave, Ottawa, ON K1A 0C6, Canada. [Wicker, Thomas] Univ Zurich, Inst Plant Biol, Zollikerstr 107, CH-8008 Zurich, Switzerland. RP Faris, JD (reprint author), USDA ARS, Cereal Crops Res Unit, USDA ARS NPA NCSL, Red River Valley Agr Res Ctr, 1605 Albrecht BLVD, Fargo, ND 58102 USA. EM Justin.Faris@ars.usda.gov FU USDA-Agricultural Research Service CRIS project [5442-22000-037-00D] FX This research was supported by USDA-Agricultural Research Service CRIS project 5442-22000-037-00D. NR 45 TC 1 Z9 1 U1 7 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 EI 1617-4623 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD FEB PY 2016 VL 291 IS 1 BP 107 EP 119 DI 10.1007/s00438-015-1091-x PG 13 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA DC1WI UT WOS:000369008300008 PM 26187026 ER PT J AU Zhang, XZ Bai, GH Xu, RG Zhang, GR AF Zhang, Xinzhong Bai, Guihua Xu, Rugen Zhang, Guorong TI Wheat streak mosaic virus resistance in eight wheat germplasm lines SO PLANT BREEDING LA English DT Article DE Wheat; Wheat streak mosaic virus; Allelic test; Inheritance; Wsm2; F-1; F-2 ID TEMPERATURE-SENSITIVE RESISTANCE; RED WINTER-WHEAT; CURL MITE; SUBSTITUTION LINE; VECTOR RESISTANCE; REGISTRATION; KANSAS; INHERITANCE; ERIOPHYIDAE; INTERMEDIUM AB Wheat streak mosaic virus (WSMV) is a destructive pathogen in wheat (Triticum aestivum L.). Host resistance is the most effective way to control this virus. To date, Wsm2 is the only wheat resistance gene that is genetically mapped. The objective of this study was to identify germplasm lines that might carry resistance genes different from Wsm2. Eight newly reported resistant germplasm lines were examined by allelic tests. To validate the allelic test results, five of them were further analysed for the inheritance of WSMV resistance. A Wsm2-linked marker was also genotyped on populations developed for the inheritance study. Our results suggested that the WSMV resistance in lines CItr9358, PI225288, PI243652, PI245439, PI245526 and PI478095 was controlled by either Wsm2 or a gene very closely linked to Wsm2. The resistance in PI243753 and PI321730, however, is likely controlled by a gene different from, but linked to Wsm2. The resistance in PI321730 might also involve some minor genes. This study provided useful information for breeders to select appropriate resistant lines to improve WSMV resistance in wheat. C1 [Zhang, Xinzhong; Zhang, Guorong] Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. [Zhang, Xinzhong; Xu, Rugen] Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Yangzhou 225009, Jiangsu, Peoples R China. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66502 USA. RP Zhang, GR (reprint author), Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. EM gzhang@ksu.edu FU Kansas Wheat Alliance; China Research System [CARS-5]; USDA National Institute of Food and Agriculture [1001453] FX This work was supported in part by the Kansas Wheat Alliance and China Research System (CARS-5). This work was also supported by the USDA National Institute of Food and Agriculture (Hatch Grant 1001453). Contribution No. is 15-411-J from the Kansas Agricultural Experiment Station. The authors thank Jeff Ackerman, Kenny Rohleder and Clayton Seaman for their valuable assistance in conducting this study. NR 46 TC 1 Z9 1 U1 0 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD FEB PY 2016 VL 135 IS 1 BP 26 EP 30 DI 10.1111/pbr.12334 PG 5 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DB9BD UT WOS:000368810000004 ER PT J AU van-Oss, RP Sherman, A Zhang, HB Vandemark, G Coyne, C Abbo, S AF van-Oss, Ruth Pinhasi Sherman, Amir Zhang, Hong-Bin Vandemark, George Coyne, Clarice Abbo, Shahal TI Vernalization response of domesticatedxwild chickpea progeny is subject to strong genotype by environment interaction SO PLANT BREEDING LA English DT Article DE adaptation; chickpea; cold hardiness; wild relatives ID ANCIENT NEAR-EAST; DIDYMELLA-RABIEI; CICER-ARIETINUM; FLOWERING TIME; CULTIVATED CHICKPEA; ASCOCHYTA BLIGHT; MAJOR GENE; EVOLUTION; LEGUMES; TEMPERATURE AB Vernalization insensitivity is a key feature of domesticated chickpea, and its genetic basis is not well understood. We studied vernalization response among hybrid progeny derived from two domesticatedxwild crosses. The wild parents are vernalization-sensitive, late-flowering genotypes while both domesticated parents are vernalization insensitive. Parental lines and hybrid progeny were tested with (28days at 4 degrees C) and without vernalization (control). The difference in mean days to flower (DTF) between control and vernalization treatments was used to assess the flowering vernalization response. A wide range of DTF values was observed among the hybrid progeny. Strong genotype by environment interaction effect on DTF was observed for the parental accessions and hybrid progeny. We used the DTF values to select vernalization responsive and non-responsive progeny lines. However, the genotypexenvironment interaction strongly interfered with our selection. Chickpea breeders interested in using the wild progenitor as a donor of exotic traits should be aware of the possibility of introducing vernalization response alleles that may alter the phenology of their breeding materials in an unpredictable manner. C1 [van-Oss, Ruth Pinhasi; Abbo, Shahal] Hebrew Univ Jerusalem, RH Smith Inst Plant Sci & Genet Agr, Levi Eshkol Sch Agr, IL-7610001 Rehovot, Israel. [Sherman, Amir] Agr Res Org, Volcani Ctr, Genom Dept, IL-50250 Bet Dagan, Israel. [Zhang, Hong-Bin] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Vandemark, George; Coyne, Clarice] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Abbo, S (reprint author), Hebrew Univ Jerusalem, RH Smith Inst Plant Sci & Genet Agr, Levi Eshkol Sch Agr, IL-7610001 Rehovot, Israel. EM shahal.abbo@mail.huji.ac.il FU BARD, The United States - Israel Binational Agricultural Research and Development Fund [IS-4427-11C] FX This research was supported in part by Research Grant No. IS-4427-11C from BARD, The United States - Israel Binational Agricultural Research and Development Fund. Shahal Abbo is the incumbent of the Jacob and Rachel Liss Chair in Agronomy. NR 36 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD FEB PY 2016 VL 135 IS 1 BP 102 EP 110 DI 10.1111/pbr.12325 PG 9 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DB9BD UT WOS:000368810000013 ER PT J AU Coskun, D Britto, DT Kochian, LV Kronzucker, HJ AF Coskun, Devrim Britto, Dev T. Kochian, Leon V. Kronzucker, Herbert J. TI How high do ion fluxes go? A re-evaluation of the two-mechanism model of k(+) transport in plant roots SO PLANT SCIENCE LA English DT Article DE Influx; Efflux; Radiotracer; Channels; Transporters; Salinity; Apoplast ID AFFINITY K+ UPTAKE; MEMBRANE-POTENTIAL CHANGES; POTASSIUM-TRANSPORTER; ARABIDOPSIS-THALIANA; SODIUM-TRANSPORT; PLASMA-MEMBRANE; BARLEY ROOTS; CORN ROOTS; RICE ROOTS; LINEAR COMPONENT AB Potassium (K+) acquisition in roots is generally described by a two-mechanism model, consisting of a saturable, high-affinity transport system (HATS) operating via H+/K+ symport at low (<1 mM) external [K+] ([K+](ext)), and a linear, low-affinity system (LATS) operating via ion channels at high (>1 MM) [K+](ext). Radiotracer measurements in the LATS range indicate that the linear rise in influx continues well beyond nutritionally relevant concentrations (>10 mM), suggesting K+ transport may be pushed to extraordinary, and seemingly limitless, capacity. Here, we assess this rise, asking whether LATS measurements faithfully report transmembrane fluxes. Using K-42(+)-isotope and electrophysiological methods in barley, we show that this flux is part of a K+-transport cycle through the apoplast, and masks a genuine plasma-membrane influx that displays Michaelis-Menten kinetics. Rapid apoplastic cycling of K+ is corroborated by an absence of transmembrane K-42(+) efflux above 1 mM, and by the efflux kinetics of PTS, an apoplastic tracer. A linear apoplastic influx, masking a saturating transmembrane influx, was also found in Arabidopsis mutants lacking the K+ transporters AtHAK5 and AtAKT1. Our work significantly revises the model of K+ transport by demonstrating a surprisingly modest upper limit for plasma-membrane influx, and offers insight into sodium transport under salt stress. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Coskun, Devrim; Britto, Dev T.; Kronzucker, Herbert J.] Univ Toronto, Dept Biol Sci, Toronto, ON M1C 1A4, Canada. [Coskun, Devrim; Britto, Dev T.; Kronzucker, Herbert J.] Univ Toronto, CCWHR, Toronto, ON M1C 1A4, Canada. [Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Kronzucker, HJ (reprint author), Univ Toronto, Dept Biol Sci, Toronto, ON M1C 1A4, Canada.; Kronzucker, HJ (reprint author), Univ Toronto, CCWHR, Toronto, ON M1C 1A4, Canada. EM devrim.coskun@mail.utoronto.ca; britto@utsc.utoronto.ca; lvk1@cornell.edu; herbert.kronzucker@utoronto.ca FU Natural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chair (CRC) program; Canadian Foundation for Innovation (CFI) FX We thank K.S.T. Poon, L. M. Schulze, and S. Gunaseelan for assistance with experiments; R. Pasuta and M. Butler (McMaster University Nuclear Reactor) for the provision of 42K and 24Na; and E. Epstein (UC Davis) for helpful discussions. This work was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canada Research Chair (CRC) program, and the Canadian Foundation for Innovation (CFI). NR 63 TC 2 Z9 2 U1 11 U2 44 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD FEB PY 2016 VL 243 BP 96 EP 104 DI 10.1016/j.plantsci.2015.12.003 PG 9 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DC1CM UT WOS:000368954400009 PM 26795154 ER PT J AU Beam, AL Thilmany, DD Pritchard, RW Garber, LP Van Metre, DC Olea-Popelka, FJ AF Beam, A. L. Thilmany, D. D. Pritchard, R. W. Garber, L. P. Van Metre, D. C. Olea-Popelka, F. J. TI Distance to slaughter, markets and feed sources used by small-scale food animal operations in the United States SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE market access; distance barriers; small farm ID POTENTIAL SPREAD; AVIAN INFLUENZA; MOUTH-DISEASE; MEAT QUALITY; CATTLE; TRANSPORT; PIGS; CONTAMINATION; MOVEMENTS; OUTBREAK AB Distances to common production and marketing supply chain destinations may vary, and this has economic and animal health implications for small-scale food animal operations. Proximity to these destinations can affect the economic viability and marketing decisions of small-scale operations and may represent significant barriers to sustainability. Data were collected using a cross-sectional survey conducted by the US Department of Agriculture's (USDA) National Animal Health Monitoring System in 2011 using a stratified systematic sample of 16,000 small-scale (gross annual farm sales between US$10,000 and 499,999) operations from all 50 states. A total of 7925 food-animal operations were asked about the farthest one-way distance (in miles) to slaughter facilities, destinations where they sold animals or products, and feed sources. Across all small-scale operations, 95% of operations reported the farthest distance animals or products were transported for sale was 241 km (150 miles) or less. For distance to slaughter facilities, 95% of operations reported the farthest distance was 145 km (90 miles) or less. For feed shipped by a supplier, 95% of operations reported the farthest distance was 322 km (200 miles) or less. The 95th percentile for distance increased as farm sales increased, indicating larger operations were more likely to travel long distances. The results of this study are an important benchmark for understanding the economic and animal health implications of long transportation distances for operations that are small and/or focused on direct marketing. C1 [Beam, A. L.; Pritchard, R. W.; Garber, L. P.] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. [Thilmany, D. D.] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. [Van Metre, D. C.; Olea-Popelka, F. J.] Colorado State Univ, Dept Clin Sci, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. RP Beam, AL (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM andrea.L.beam@aphis.usda.gov NR 39 TC 1 Z9 1 U1 4 U2 14 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD FEB PY 2016 VL 31 IS 1 BP 49 EP 59 DI 10.1017/S1742170514000441 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA DC2YT UT WOS:000369085700007 ER PT J AU Liu, MY Lei, L Powers, C Liu, ZY Campbell, KG Chen, XM Bowden, RL Carver, BF Yan, LL AF Liu, Meiyan Lei, Lei Powers, Carol Liu, Zhiyong Campbell, Kimberly G. Chen, Xianming Bowden, Robert L. Carver, Brett F. Yan, Liuling TI TaXA21-A1 on chromosome 5AL is associated with resistance to multiple pests in wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID TRITICUM-AESTIVUM L.; STRIPE RUST RESISTANCE; PATTERN-RECOGNITION RECEPTOR; F-SP TRITICI; DISEASE RESISTANCE; HEXAPLOID WHEAT; XA21-MEDIATED IMMUNITY; JUXTAMEMBRANE DOMAIN; HESSIAN FLY; BREAD WHEAT AB Key message The wheat ortholog of the rice gene OsXA21 against bacterial leaf blight showed resistance to multiple pests in bread wheat but different interacting proteins. A quantitative trait locus QYr.osu-5A on the long arm of chromosome 5A in bread wheat (Triticum aestivum L., 2n = 6x = 42; AABBDD) was previously reported to confer consistent resistance in adult plants to predominant stripe rust races, but the gene causing the quantitative trait locus (QTL) is not known. Single-nucleotide polymorphism (SNP) markers were used to saturate the QTL region. Comparative and syntenic regions between wheat and rice (Oryza sativa) were applied to identify candidate genes for QYr.osu-5A. TaXA21-A1, which is referred to as a wheat ortholog of OsXA21-like gene on chromosome 9 in rice, was mapped under the peak of the QYr.osu-5A. TaXA21-A1 not only explained the phenotypic variation in reaction to different stripe rust races but also showed significant effects on resistance to powdery mildew and Hessian fly biotype BP. The natural allelic variation resulted in the alternations of four amino acids in deduced TaXA21-A1 proteins. The interacting proteins of TaXA21-A1 were different from those identified by OsXA21 on rice chromosome 11 against bacterial leaf blight. TaXA21-A1 confers unique resistance against multiple pests in wheat but might not have common protein interactors or thus overlapping functions with OsXA21 in rice. XA21 function has diverged during evolution of cereal crops. The molecular marker developed for TaXA21-A1 would accelerate its application of the candidate gene at the QYr.osu-5A locus in wheat breeding programs. C1 [Liu, Meiyan; Lei, Lei; Powers, Carol; Carver, Brett F.; Yan, Liuling] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74074 USA. [Liu, Meiyan] Jiangsu Normal Univ, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China. [Liu, Zhiyong] China Agr Univ, Dept Plant Genet & Breeding, Beijing 100193, Peoples R China. [Campbell, Kimberly G.; Chen, Xianming] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Campbell, Kimberly G.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Bowden, Robert L.] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Yan, LL (reprint author), Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74074 USA. EM liuling.yan@okstate.edu FU USDA-NIFA T-CAP grant [2011-68002-30029]; Oklahoma Wheat Research Foundation FX This work was supported by USDA-NIFA T-CAP grant No. 2011-68002-30029, and the Oklahoma Wheat Research Foundation. NR 52 TC 0 Z9 0 U1 5 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2016 VL 129 IS 2 BP 345 EP 355 DI 10.1007/s00122-015-2631-9 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DC1QJ UT WOS:000368991100011 PM 26602233 ER PT J AU Fakthongphan, J Bai, GH St Amand, P Graybosch, RA Baenziger, PS AF Fakthongphan, Juthamas Bai, Guihua St Amand, Paul Graybosch, R. A. Baenziger, P. S. TI Identification of markers linked to genes for sprouting tolerance (independent of grain color) in hard white winter wheat (HWWW) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; BREAD-WHEAT; SEED DORMANCY; PREHARVEST; RESISTANCE; MICROSATELLITE; CROSS; REGISTRATION; RICE; CHROMOSOMES AB Key message Hard red wheats can donate genes to hard white wheats for tolerance to preharvest sprouting, the effects are quantitative in nature, and may be tracked with previously described DNA markers. Pre-harvest sprouting (PHS) of wheat (Triticum aestivum L.) can negatively impact end-use quality and seed viability at planting. Due to preferences for white over red wheat in international markets, white wheat with PHS tolerance has become increasingly desired for worldwide wheat production. In general, however, red wheat is more tolerant of sprouting than white wheat. The main objective of this study was the identification of PHS tolerance conditioned by genes donated from hard red winter wheat, using markers applicable to the Great Plains hard white wheat gene pool. Three red wheat by white wheat populations, Niobrara/NW99L7068, NE98466/NW99L7068 and Jagalene/NW99L7068 were developed, and white-seeded progenies were analyzed for PHS tolerance and used to identify markers for the trait. In the three populations, marker loci with significant allelic effects were most commonly located on chromosomes of group 2, 3, 4 and 5, though additional markers were detected across the wheat genome. Chromosome 3A was the only chromosome with significant markers in all three populations. Markers were inconsistent across the three populations, and markers linked to tolerance-inducing loci were identified in both tolerant and susceptible parents. Additive effects of marker loci were common. In the present investigation, a wide range of PHS tolerance was observed, even though all lines were fixed for the recently reported positive TaPHS1 allele. PHS tolerance is controlled by additive major gene effects with minor gene effects where variations of minor gene effects were still unclear. C1 [Fakthongphan, Juthamas; Graybosch, R. A.; Baenziger, P. S.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Bai, Guihua; St Amand, Paul] Kansas State Univ, Hard Winter Wheat Genet Res Unit, USDA ARS, Manhattan, KS 66506 USA. [Graybosch, R. A.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RP Graybosch, RA (reprint author), Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. EM Bob.Graybosch@ARS.USDA.GOV NR 43 TC 2 Z9 2 U1 4 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2016 VL 129 IS 2 BP 419 EP 430 DI 10.1007/s00122-015-2636-4 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DC1QJ UT WOS:000368991100016 PM 26687145 ER PT J AU Naranjo, SE Li, SJ AF Naranjo, Steven E. Li, Shujuan TI Long term dynamics of aphelinid parasitoids attacking Bemisia tabaci SO BIOLOGICAL CONTROL LA English DT Article DE Eretmocerus spp.; Encarsia spp.; Classical biological control; Parasitism; Species composition; Displacement ID GUT CONTENT ELISA; NATURAL ENEMIES; BIOLOGICAL-CONTROL; INSECTICIDE TREATMENTS; COTTON; ALEYRODIDAE; HOMOPTERA; POPULATIONS; HYMENOPTERA; CONSERVATION AB Aphelinid parasitoids are widely known natural enemies of Bemisia tabaci (Gennadius), a serious pest of agriculture globally. Here we examine pest-parasitoid interactions and dynamics in Arizona cotton from 1996 to 2010, during which a classical biological control program was implemented. Two native species, Eretmocerus eremicus Rose and Zolnerowich and Encarsia meritoria (Gahan) were either largely or completely displaced by exotic Eretmocerus sp. (Ethiopia) and Encarsia sophia (Gahan) in the early 2000s. Further, E. sophia became the dominant parasitoid of B. tabaci in cotton after many years of predominance by native and exotic Eretmocerus. Apparent rates of parasitism were highly variable within and between years and averaged overall. In some years there was evidence that B. tabaci populations declined as apparent parasitism increased. Lower pest abundance was associated with higher rates of apparent parasitism over the entire 15-year period but this pattern was not supported by long term life-table based measurements of parasitism. Detailed life table studies within the entire agro-ecosystem will be needed to fully assess the impact of the classical biological control program. Published by Elsevier Inc. C1 [Naranjo, Steven E.] ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. [Li, Shujuan] Univ Arizona, Maricopa Agr Ctr, 37860 West Smith Enke Rd, Maricopa, AZ 85138 USA. RP Naranjo, SE (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. EM steve.naranjo@ars.usda.gov NR 51 TC 0 Z9 0 U1 7 U2 28 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD FEB PY 2016 VL 93 BP 56 EP 64 DI 10.1016/j.biocontrol.2015.11.010 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DB5KU UT WOS:000368553800008 ER PT J AU Mourtzinis, S Cantrell, KB Arriaga, FJ Balkcom, KS Novak, JM Frederick, JR Karlen, DL AF Mourtzinis, Spyridon Cantrell, Keri B. Arriaga, Francisco J. Balkcom, Kipling S. Novak, Jeff M. Frederick, James R. Karlen, Douglas L. TI Carbohydrate and nutrient composition of corn stover from three southeastern USA locations SO BIOMASS & BIOENERGY LA English DT Article DE Theoretical ethanol yield; Nutrient removal; Corn; Carbohydrates; Stover removal ID RESIDUE REMOVAL; ORGANIC-MATTER; BIOMASS YIELD; SOIL; MANAGEMENT; FEEDSTOCK; TILLAGE; CROP; DEGRADATION; NITROGEN AB Corn (Zea mays L.) stover has been identified as an important feedstock for bioenergy and bio-product production. Our objective was to quantify nutrient removal, carbohydrate composition, theoretical ethanol yield (TEY) for various stover fractions. In 2009, 2010, and 2011, whole-plant samples were collected from one field study in South Carolina (SC) and two in Alabama (AL). Soils at the SC site were classified as a Coxville/Rains-Goldboro-Lynchburg association, while those in AL were either Compass or Decatur. Plants were collected from two 1-m row segments, ears were removed and shelled. A portion of the remaining stalks were dried and ground to represent whole-plant stover. The remaining stalks were fractionated into stalk and leaf biomass from below the ear (bottom), stalk and leaf biomass from above the ear (top), cobs, and grain. A fifth sample representing "above-ear" biomass that might be collected mechanically was calculated using the weight ratios of the top and cob fractions. Carbohydrate and nutrient concentrations were estimated using near-infrared spectroscopy (NIRS) and TEY was calculated. The distribution of carbohydrates, nutrients, and TEY varied significantly among the corn stover fraction and research locations. This indicates that site-specific sampling and analysis should be used to optimize bioenergy and bio-product utilization of corn stover. However, at every location, the above-ear stover fractions were most desirable for cellulosic ethanol production. Furthermore, harvesting only above-ear stover fractions would reduce nutrient removal by 24-61% when compared to harvesting all stover biomass. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Mourtzinis, Spyridon] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Cantrell, Keri B.; Novak, Jeff M.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Arriaga, Francisco J.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. [Balkcom, Kipling S.] ARS, Natl Soil Dynam Res Lab, USDA, Auburn, AL 36832 USA. [Frederick, James R.] Clemson Univ, Pee Dee Res & Educ Ctr, Florence, SC 29501 USA. [Karlen, Douglas L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Mourtzinis, S (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM mourtzinis@wisc.edu FU Alabama Wheat and Feed Grain Producers Commission of the Alabama Farmers Federation; North Central Regional Sun Grant Center at South Dakota State University through USDOE - Office of Biomass Programs [DE-FC36-05GO85041] FX The authors would like to thank the many hands aiding in the corn planting, harvest collections, sample preparations and analyses. Partial funding for this research was provided by the Alabama Wheat and Feed Grain Producers Commission of the Alabama Farmers Federation. This study is a contribution of the multi-location USDA-ARS-REAP project, supported in part by supplemental funding from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the USDOE - Office of Biomass Programs under award number DE-FC36-05GO85041. Mention of a trade name, proprietary product, or vendors is for information only and does not guarantee or warrant the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 33 TC 0 Z9 0 U1 4 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD FEB PY 2016 VL 85 BP 153 EP 158 DI 10.1016/j.biombioe.2015.11.031 PG 6 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DB3TW UT WOS:000368435800019 ER PT J AU Zhang, ZL Zhang, T Zhang, AJ Luo, J Chen, LZ Wang, MQ Ning, J Lei, CL AF Zhang, Zhilin Zhang, Tao Zhang, Aijun Luo, Jing Chen, Lizhen Wang, Manqun Ning, Jun Lei, Chaoliang TI Identification and field verification of sex pheromone from the mirid bug, Adelphocoris suturalis SO CHEMOECOLOGY LA English DT Article DE Metathoracic scent gland; (E)-4-oxo-2-hexenal; Hexyl hexanoate; Hexyl butyrate; Olfactometer bioassay; Field test; Integrated pest management ID STENOTUS-RUBROVITTATUS MATSUMURA; TARNISHED PLANT BUG; BT COTTON; LYGOCORIS-PABULINUS; HETEROPTERA MIRIDAE; LYGUS-RUGULIPENNIS; SEASONAL ABUNDANCE; HEMIPTERA-MIRIDAE; COMPONENTS; FEMALE AB Numerous species of mirid bugs (Hemiptera: Miridae) are phytophagous insect pests that damage economically important field and horticultural crops. In recent years, Adelphocoris suturalis has become a significant pest in transgenic Bacillus thuringiensis (Bt) cotton fields. We previously reported that virgin A. suturalis females attracted males in the field. In this study, we found that A. suturalis males are not attracted in Y-tube olfactometer bioassays to odors from either live nymphs or sexually mature virgin females whose ostioles of the metathoracic scent gland (MTG) are occluded with paraffin wax. However, males are significantly attracted to odors from adult virgin females when female abdomina are occluded with paraffin, indicating that female A. suturalis release sex pheromones only from the MTG. Volatile extracts from both sexes were collected and analyzed by gas chromatography-electroantennogram detection and gas chromatography-mass spectrometry. We identified (E)-4-oxo-2-hexenal (E4O2H), hexyl hexanoate (HH) and hexyl butyrate (HB) as major components and found that they elicited the largest EAD responses from antennae of males. Both E4O2H and HH were present in aeration extracts from females, while E4O2H was found in males. Capture of only males in field tests using synthetic compounds confirmed the activity of the identified sex pheromone candidates. In the field tests, a 10:1 ratio of the E4O2H and HH binary blend exhibited the highest efficiency in attracting male A. suturalis. Addition of HB to the binary pheromone blend to make a ternary blend did not increase trap catches. These results demonstrate that E4O2H and HH constitute the A. suturalis sex pheromone components. C1 [Zhang, Zhilin; Luo, Jing; Chen, Lizhen; Wang, Manqun; Lei, Chaoliang] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China. [Zhang, Tao; Ning, Jun] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Zhang, Aijun] USDA ARS, BARC West, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Chen, LZ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China.; Ning, J (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM lzchen@mail.hzau.edu.cn; jning502@yahoo.com.cn FU Special Fund for Agri-scientific Research in the Public Interest, from the Chinese Ministry of Agriculture [201103012] FX We thank Dr Joe Hull (Pest Management & Biological Control Research Unit, USDA-ARS, Arid-Land Agricultural Research Center) for the full language editing of this manuscript. We also thank Drs. Weihua Ma and Xiaoyun Wang for improving our manuscript. This work was supported by the Special Fund for Agri-scientific Research in the Public Interest, from the Chinese Ministry of Agriculture (Grant 201103012). NR 32 TC 0 Z9 0 U1 5 U2 20 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 0937-7409 EI 1423-0445 J9 CHEMOECOLOGY JI Chemoecology PD FEB PY 2016 VL 26 IS 1 BP 25 EP 31 DI 10.1007/s00049-015-0203-8 PG 7 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DB6OO UT WOS:000368634500004 ER PT J AU Kim, M Wells, JE AF Kim, Minseok Wells, James. E. TI A Meta-analysis of Bacterial Diversity in the Feces of Cattle SO CURRENT MICROBIOLOGY LA English DT Article ID BEEF-CATTLE; SP-NOV.; FECAL MICROBIOTA; COW FECES; GEN-NOV.; TAXONOMY; DIETS; SOLUBLES; RUMEN AB In this study, we conducted a meta-analysis on 16S rRNA gene sequences of bovine fecal origin that are publicly available in the RDP database. A total of 13,663 sequences including 603 isolate sequences were identified in the RDP database (Release 11, Update 1), where 13,447 sequences were assigned to 10 phyla, 17 classes, 28 orders, 59 families, and 110 genera, while the remaining 216 sequences could not be assigned to a known phylum. Firmicutes and Bacteroidetes were the first and the second predominant phyla, respectively. About 41 % of the total sequences could not be assigned to a known genus. The total sequences were assigned to 1252 OTUs at 97 % sequence similarity. A small number of OTUs shared among datasets indicate that fecal bacterial communities of cattle are greatly affected by various factors, specifically diet. This study may guide future studies to further analyze fecal bacterial communities of cattle. C1 [Kim, Minseok; Wells, James. E.] USDA, US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. [Kim, Minseok] Natl Inst Anim Sci, Anim Nutr & Physiol Team, RDA, Wanju Gun, Jeollabuk Do, South Korea. RP Wells, JE (reprint author), USDA, US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM Jim.Wells@ars.usda.gov NR 27 TC 2 Z9 2 U1 7 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 EI 1432-0991 J9 CURR MICROBIOL JI Curr. Microbiol. PD FEB PY 2016 VL 72 IS 2 BP 145 EP 151 DI 10.1007/s00284-015-0931-6 PG 7 WC Microbiology SC Microbiology GA DB6PO UT WOS:000368637300006 PM 26542532 ER PT J AU Tarpy, DR Simone-Finstrom, M Linksvayer, TA AF Tarpy, D. R. Simone-Finstrom, M. Linksvayer, T. A. TI Honey bee colonies regulate queen reproductive traits by controlling which queens survive to adulthood SO INSECTES SOCIAUX LA English DT Article DE Social physiology; Colony-level selection; Royal cheats; Queen reproductive potential ID APIS-MELLIFERA; LARVAL; MAINTENANCE; EVOLUTION; QUALITY; WORKERS; NUMBER; BROOD; AGE AB The production of new queens in honey bee colonies is one of the most important determinants of reproductive success, and it involves cooperative behavior among hundreds or thousands of workers. Colony members are generally expected to benefit by optimizing the reproductive traits of prospective replacement queens, but potential conflicts of interest among colony members could result in suboptimal queens. We studied the degree to which colonies regulate adult queen traits by controlling access to developing queens that survived from pupation to adulthood. We also searched for evidence of strong conflict among patrilines by comparing the contribution of patrilines to new queens and new workers, although we found no evidence for the existence of significantly queen-biased patrilines or for any association between patriline contribution to new queens and queen traits. However, adult queens emerging from cells accessible to workers were larger in terms of compared to adult queens emerging from cells that were not accessible to workers. These results suggest that colonies regulate queen quality traits by curtailing low-quality queens from fully developing, which is further evidence that cooperation predominates over potential conflict within honey bee colonies. C1 [Tarpy, D. R.; Simone-Finstrom, M.] N Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA. [Tarpy, D. R.; Simone-Finstrom, M.] N Carolina State Univ, WM Keck Ctr Behav Biol, Campus Box 7613, Raleigh, NC 27695 USA. [Simone-Finstrom, M.; Linksvayer, T. A.] Univ Penn, Dept Biol, 225 Leidy Labs,433 South Univ Ave, Philadelphia, PA 19104 USA. [Simone-Finstrom, M.] USDA ARS, Honey Bee Breeding Genet & Physiol Res, Baton Rouge, LA 70820 USA. RP Tarpy, DR (reprint author), N Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA.; Tarpy, DR (reprint author), N Carolina State Univ, WM Keck Ctr Behav Biol, Campus Box 7613, Raleigh, NC 27695 USA. EM david_tarpy@ncsu.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2014-67013-21725]; NC Department of Agriculture and Consumer Services FX Shiquita Toney assisted with the fieldwork and data collection, and Joel Caren helped with genotyping the workers and queens. This material is based on work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Award Number 2014-67013-21725, as well as to DRT by the NC Department of Agriculture and Consumer Services. NR 30 TC 0 Z9 0 U1 9 U2 30 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 0020-1812 EI 1420-9098 J9 INSECT SOC JI Insect. Soc. PD FEB PY 2016 VL 63 IS 1 BP 169 EP 174 DI 10.1007/s00040-015-0452-0 PG 6 WC Entomology SC Entomology GA DB6RA UT WOS:000368641500019 ER PT J AU Tunick, MH Van Hekken, DL Paul, M Ingham, ER Karreman, HJ AF Tunick, Michael H. Van Hekken, Diane L. Paul, Moushumi Ingham, Elaine R. Karreman, Hubert J. TI Case study: Comparison of milk composition from adjacent organic and conventional farms in the USA SO INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY LA English DT Article DE CLA; Linolenic acid; Omega-3 fatty acids; Organic milk ID CONJUGATED LINOLEIC-ACID; POLYUNSATURATED FATTY-ACIDS; LACTATING DAIRY-COWS; FISH-OIL; PASTURE; SYSTEMS; CLA; MINERALS AB A study of two adjacent dairy farms, one using conventional confined herd management and the other organic management, revealed significant differences in the fatty acid composition of the milk. Compared with conventional milk, organic milk had higher levels of conjugated linoleic acid (CLA) and -linolenic acid (the major omega-3 fatty acid in milk), and less stearic and linoleic acid (the major omega-6 fatty acid in milk) during the spring-summer grazing season. When discarding geography and weather as variables, organic milk appears to yield more CLA and -linolenic acid, which should be beneficial to health. C1 [Tunick, Michael H.; Van Hekken, Diane L.; Paul, Moushumi] USDA ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Ingham, Elaine R.] Soil Foodweb Inc, 1750 SW 3rd St C, Corvallis, OR 97333 USA. [Karreman, Hubert J.] Rodale Inst, 611 Sigfriedale Rd, Kutztown, PA 19530 USA. RP Tunick, MH (reprint author), USDA ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM michael.tunick@ars.usda.gov NR 36 TC 0 Z9 0 U1 9 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-727X EI 1471-0307 J9 INT J DAIRY TECHNOL JI Int. J. Dairy Technol. PD FEB PY 2016 VL 69 IS 1 BP 137 EP 142 DI 10.1111/1471-0307.12284 PG 6 WC Food Science & Technology SC Food Science & Technology GA DB5TQ UT WOS:000368576900017 ER PT J AU Brent, CS Miyasaki, K Vuong, C Miranda, B Steele, B Brent, KG Nath, R AF Brent, Colin S. Miyasaki, Katelyn Vuong, Connor Miranda, Brittany Steele, Bronwen Brent, Kristoffer G. Nath, Rachna TI Regulatory roles of biogenic amines and juvenile hormone in the reproductive behavior of the western tarnished plant bug (Lygus hesperus) SO JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY LA English DT Article DE Lygus hesperus; Biogenic amines; Juvenile hormone; Sexual receptivity; Oviposition ID FEMALE SEXUAL RECEPTIVITY; HONEYBEE APIS-MELLIFERA; CORPORA ALLATA ACTIVITY; DROSOPHILA-MELANOGASTER; HELIOTHIS-VIRESCENS; HEMIPTERA-MIRIDAE; STRESS CONDITIONS; VISCERAL MUSCLE; LOCUST OVIDUCTS; MUSHROOM BODIES AB Mating induces behavioral and physiological changes in the plant bug Lygus hesperus Knight (Hemiptera: Miridae). After receiving seminal products, which include the systemic regulator juvenile hormone (JH), females enter a post-mating period lasting several days during which they enhance their oviposition rate and lose interest in remating. To elucidate the regulation of these behavioral changes in L. hesperus, biogenic amines were quantified in the heads of females at 5 min, 1 h and 24 h after copulation and compared to levels in virgins using high-performance liquid chromatography coupled with electrochemical detection. Mating significantly increased dopamine (DA) after 1 and 24 h, and decreased octopamine (OA) after 5 min and 1 h. Serotonin did not change with mating, but tyramine was significantly reduced after 5 min. While injection of amines into virgin females did not influence sexual receptivity, OA caused a decrease in oviposition during the 24 h following injection. Topical application of the JH analog methoprene to virgins caused an increase in DA, and a decline in mating propensity, but did not influence other amines or the oviposition rate. The results suggest the decline in OA observed immediately after mating may promote egg laying, and that male-derived JH may induce an increase in DA that could account for the post-mating loss of sexual receptivity. C1 [Brent, Colin S.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Miyasaki, Katelyn; Vuong, Connor] Desert Vista High Sch, Phoenix, AZ USA. [Miranda, Brittany; Steele, Bronwen] Estrella Mt Community Coll, Div Sci, Avondale, AZ USA. [Brent, Kristoffer G.] Kristiansand Katedralskole Gimle, Kristiansand, Norway. [Nath, Rachna] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. RP Brent, CS (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM colin.brent@ars.usda.gov OI Brent, Colin/0000-0003-2078-1417 FU Agriculture and Food Research Initiative Competitive Grant from the United States Department of Agriculture (USDA), National Institute of Food and Agriculture [2011-38422-30955] FX We thank Dan Langhorst for technical assistance. This project was supported by Agriculture and Food Research Initiative Competitive Grant No. 2011-38422-30955 from the United States Department of Agriculture (USDA), National Institute of Food and Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 73 TC 2 Z9 2 U1 3 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0174-1578 EI 1432-136X J9 J COMP PHYSIOL B JI J. Comp. Physiol. B-Biochem. Syst. Environ. Physiol. PD FEB PY 2016 VL 186 IS 2 BP 169 EP 179 DI 10.1007/s00360-015-0953-1 PG 11 WC Physiology; Zoology SC Physiology; Zoology GA DB6RN UT WOS:000368642800003 PM 26686231 ER PT J AU Maki, CR Thomas, AD Elmore, SE Romoser, AA Harvey, RB Ramirez-Ramirez, HA Phillips, TD AF Maki, C. R. Thomas, A. D. Elmore, S. E. Romoser, A. A. Harvey, R. B. Ramirez-Ramirez, H. A. Phillips, T. D. TI Effects of calcium montmorillonite clay and aflatoxin exposure on dry matter intake, milk production, and milk composition SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE aflatoxin; food safety; milk vitamins; mycotoxins ID DAIRY-COWS; CONTAMINATED DIETS; NOVASIL CLAY; B-1; M-1; ALUMINOSILICATE; RIBOFLAVIN; ABSORPTION; REDUCTION; EXTRACTS AB Fifteen primiparous crossbred dairy cows that were 114 +/- 14 d in milk and weighed 533 +/- 56 kg were used in a replicated 5 x 5 Latin square to test the efficacy of a calcium montmorillonite clay, NovaSil Plus (NSP; BASF Corp., Ludwigshaven, Germany), for the reduction of aflatoxin (AF) metabolite (AFM(1)) in milk and the effect of NSP on milk composition. Cows were housed in a freestall barn, fed once a day and milked twice a day. The experiment consisted of five 14-d periods: d 1 through 7 were considered for data collection, and d 8 through 14 were considered a wash-out phase. In each period, cows were randomly assigned to 1 of 5 dietary treatments: (1) control (CON), consisting of a basal total mixed ration (TMR); (2) high-dose NSP diet (NSP-1%), consisting of TMR plus 230 g of NSP; (3) aflatoxin diet (AFD), consisting of the TMR plus AF challenge; (4) low-dose NSP with AF (NSP-0.5%+AFD), composed of TMR plus 115 g of NSP and AF challenge; and (5) high-dose NSP with AF (NSP-1%+AFD), consisting of TMR plus 230 g of NSP and AF challenge. The AF challenge consisted of top dressing a daily dose of 100 mu g/kg estimated, dry matter intake (DMI); similarly, NSP was fed at 1.0 or 0.5% of estimated DMI. Milk yield and DMI were similar across treatments averaging 21.1 +/- 1.33 kg/d and 19.7 +/- 0.56 kg/d, respectively. Concentration of milk fat, protein, and lactose were similar across treatments with averages of 4.91 +/- 0.20%, 3.85 +/- 0.10%, and 4.70 +/- 0.06%, respectively. Concentration of vitamin A averaged 0.28 +/- 0.03 mu g/mL and riboflavin concentration averaged 1.57 +/- 0.13 mu g/mL across treatments. The concentration of minerals in milk were similar for all treatments. Cows fed CON and NSP-1% yielded the lowest concentration of AFM1 in milk with 0.03 and 0.01 +/- 0.06 mu g/L. Addition of NSP reduced milk AFM(1). from 1.10 +/- 0.06 mu g/L with the AF diet to 0.58 and 0.32 +/- 0.06 mu g/L with the NSP-0.5%+AF and NSP-1%+AF diets, respectively. Excretion of AFM1 was reduced by NSF; mean values were 24.38, 11.86, 7.38, 0.64, and 0.23, +/- 1.71 mu g/d, for AFD, NSP-0.5%+AFD, NSP-1%+AFD, NSP-1%, and CON, respectively. More specifically, 1.07 +/- 0.08% of the daily AF intake was transferred to the milk of cows consuming the AFD, whereas the AF transfer rates in milk from cows that consumed the NSP-0.5%+AFD and NSP-1%+AFD were 0.52 and 0.32 +/- 0.08%. Results from this research demonstrate that feeding NSP to lactating cows is an effective method to reduce the transfer and excretion of AFM1 in milk with no negative effects on dry matter intake, milk production, and composition. C1 [Maki, C. R.; Elmore, S. E.; Romoser, A. A.; Phillips, T. D.] Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Thomas, A. D.; Ramirez-Ramirez, H. A.] Tarleton State Univ, Dept Anim Sci & Vet Technol, Stephenville, TX 76402 USA. [Harvey, R. B.] ARS, Food & Feed Safety Res, USDA, College Stn, TX 77845 USA. [Harvey, R. B.; Ramirez-Ramirez, H. A.; Phillips, T. D.] Texas A&M AgriLife Res, College Stn, TX 77843 USA. RP Phillips, TD (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. EM tphillips@cvm.tamu.edu OI Ramirez Ramirez, Hugo Alonso/0000-0002-5074-9277 FU Aflatoxin Mitigation Center of Excellence Research Program (National Corn Growers Association, St. Louis, MO) [M1403049] FX This work was supported by funding through the Aflatoxin Mitigation Center of Excellence Research Program (National Corn Growers Association, St. Louis, MO), M1403049. NR 35 TC 2 Z9 2 U1 6 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD FEB PY 2016 VL 99 IS 2 BP 1039 EP 1046 DI 10.3168/jds.2015-10242 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DB4FH UT WOS:000368468400015 PM 26709170 ER PT J AU Gaddis, KLP Cole, JB Clay, JS Maltecca, C AF Gaddis, K. L. Parker Cole, J. B. Clay, J. S. Maltecca, C. TI Benchmarking dairy herd health status using routinely recorded herd summary data SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE herd health status; producer-recorded data; prediction; benchmarking ID RISK-FACTORS; CLINICAL MASTITIS; MILK-PRODUCTION; LACTATING COWS; UNITED-STATES; DANISH DAIRY; HEAT-STRESS; MANAGEMENT; DISEASE; FARM AB Genetic improvement of dairy cattle health through the use of producer-recorded data has been determined to be feasible. Low estimated heritabilities indicate that genetic progress will be slow. Variation observed in lowly heritable traits can largely be attributed to nongenetic factors, such as the environment. More rapid improvement of dairy cattle health may be attainable if herd health programs incorporate environmental and managerial aspects. More than 1,100 herd characteristics are regularly recorded on farm test-days. We combined these data with producer-recorded health event data, and parametric and nonparametric models were used to benchmark herd and cow health status. Health events were grouped into 3 categories for analyses: mastitis, reproductive, and metabolic. Both herd incidence and individual incidence were used as dependent variables. Models implemented included stepwise logistic regression, support vector machines, and random forests. At both the herd and individual levels, random forest models attained the highest accuracy for predicting health status in all health event categories when evaluated with 10-fold cross-validation. Accuracy (SD) ranged from 0.61 (0.04) to 0.63 (0.04) when using random forest models at the herd level. Accuracy of prediction (SD) at the individual cow level ranged from 0.87 (0.06) to 0.93 (0.001) with random forest models. Highly significant variables and key words from logistic regression and random forest models were also investigated. All models identified several of the same key factors for each health event category, including movement out of the herd, size of the herd, and weather-related variables. We concluded that benchmarking health status using routinely collected herd data is feasible. Nonparametric models were better suited to handle this complex data with numerous variables. These data mining techniques were able to perform prediction of health status and could add evidence to personal experience in herd management. C1 [Gaddis, K. L. Parker] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Cole, J. B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Clay, J. S.] Dairy Records Management Syst, Raleigh, NC 27603 USA. [Maltecca, C.] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. RP Gaddis, KLP (reprint author), Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. EM klpgaddis@ufl.edu RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Agricultural Research Service of the United States Department of Agriculture [1265-31000-096-00] FX The authors thank Dairy Records Management Systems (Raleigh, NC) and the Council on Dairy Cattle Breeding (Reynoldsburg, OH) for providing the data used in this study, as well as Cai Li and Ravi Mathur from the Bioinformatics Department at North Carolina State University for their contributions with initial model selection. J. B. Cole was supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information," of the Agricultural Research Service of the United States Department of Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 52 TC 1 Z9 1 U1 1 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD FEB PY 2016 VL 99 IS 2 BP 1298 EP 1314 DI 10.3168/jds.2015-9840 PG 17 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DB4FH UT WOS:000368468400039 ER PT J AU Smith, RL Grohn, YT Pradhan, AK Whitlock, RH Van Kessel, JS Smith, JM Wolfgang, DR Schukken, YH AF Smith, Rebecca L. Groehn, Y. T. Pradhan, A. K. Whitlock, R. H. Van Kessel, J. S. Smith, J. M. Wolfgang, D. R. Schukken, Y. H. TI The effects of progressing and nonprogressing Mycobacterium avium ssp paratuberculosis infection on milk production in dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE paratuberculosis; Johne's disease; milk production ID JOHNES-DISEASE STATUS; HERDS; TUBERCULOSIS; REPRODUCTION; LACTATION; CATTLE; IMPACT; MODEL AB Longitudinal data from 3 commercial dairy herds in the northeast United States, collected from 2004 to 2011, were analyzed to determine the effect of Mycobacterium avium ssp. paratuberculosis (MAP) infection status and progression path on milk production. Disease status, as indicated by MAP test results, was determined through quarterly ELISA serum testing, biannual fecal culture, and culture of tissues and feces at slaughter. Milk production data were collected from the Dairy Herd Information Association. Animals with positive MAP test results were categorized, based on test results over the full course of the study, as high path (at least one high-positive culture) or low path (at least one positive culture or ELISA). The cumulative numbers of positive ELISA and culture results were recorded. The effects of both MAP infection path, status, and number of positive tests on milk production were analyzed using a mixed linear model with an autocorrelation random effect structure. Low- and high-path animals produced more milk before their first positive test than always-negative animals, especially high-path animals. Although mean production decreased after a first positive test, low-path animals were shown to recover some productivity. High-path animals continued to exhibit a decrease in milk production, especially after their first high-positive fecal culture. These results show that not all animals that test positive for MAP will have long-term production losses. Milk production decreased significantly with each additional positive test. Ultimately, production loss appeared to be a function of MAP infection progression. C1 [Smith, Rebecca L.] Univ Illinois, Coll Vet Med, Dept Pathobiol, Urbana, IL 61802 USA. [Groehn, Y. T.; Schukken, Y. H.] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14850 USA. [Pradhan, A. K.] Univ Maryland, Coll Agr & Nat Resources, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Pradhan, A. K.] Univ Maryland, Coll Agr & Nat Resources, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Whitlock, R. H.] Univ Penn, Sch Vet Med, Dept Clin Studies, New Bolton Ctr, Kennett Sq, PA 19348 USA. [Van Kessel, J. S.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Smith, J. M.] Univ Vermont, Dept Anim & Vet Sci, Burlington, VT 05405 USA. [Wolfgang, D. R.] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Schukken, Y. H.] GD Anim Hlth, NL-7400 AA Deventer, Netherlands. RP Smith, RL (reprint author), Univ Illinois, Coll Vet Med, Dept Pathobiol, Urbana, IL 61802 USA. EM rlsdvm@illinois.edu FU Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health (Bethesda, MD) [8K01OD01968-04]; National Institute of Food and Agriculture of the United States Department of Agriculture (Washington, DC) through NIFA Award [2014-67015-2240] FX The authors gratefully acknowledge funding provided by the Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health (Bethesda, MD) through Grant Number 8K01OD01968-04 and the National Institute of Food and Agriculture of the United States Department of Agriculture (Washington, DC) through NIFA Award No. 2014-67015-2240. NR 22 TC 1 Z9 1 U1 1 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD FEB PY 2016 VL 99 IS 2 BP 1383 EP 1390 DI 10.3168/jds.2015-9822 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DB4FH UT WOS:000368468400046 PM 26686721 ER PT J AU Lin, JT Fagerquist, CK Chen, GQ AF Lin, Jiann-Tsyh Fagerquist, Clifton K. Chen, Grace Q. TI Ratios of Regioisomers of the Molecular Species of Triacylglycerols in Lesquerella (Physaria fendleri) Oil Estimated by Mass Spectrometry SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Lesquerella oil; Physaria fendleri; Triacylglycerols; Regioisomers; Quantification; Mass spectrometry ID ACYLGLYCEROLS LESS POLAR; COMMUNIS L. OIL; CASTOR-OIL; FATTY-ACIDS; LITHIATED ADDUCTS; IDENTIFICATION; QUANTIFICATION; HPLC; TRIRICINOLEIN; DAG AB The ratios of regioisomers of 72 molecular species of triacylglycerols (TAG) in lesquerella oil were estimated using the electrospray ionization mass spectrometry of the lithium adducts of TAG in the HPLC fractions of lesquerella oil. The ratios of ion signal intensities (or relative abundances) of the fragment ions from the neutral losses of fatty acids (FA) as alpha-lactones at the sn-2 position (MS3) of the molecular species of TAG were used as the ratios of the regioisomers. The order of the preference of FA incorporation at the sn-2 position of the molecular species of TAG in lesquerella was as: normal FA > OH18 (monohydroxy FA with 18 carbon atoms) > diOH18 > OH20 > diOH20, while in castor was as: normal FA > OH18 > OH20 > diOH18 > triOH18. Elongation (from C-18 to C-20) was more effective than hydroxylation in lesquerella to incorporate hydroxy FA at the sn-1/3 positions. The block of elongation in lesquerella may be used to increase the content of hydroxy FA, e.g., ricinoleate, at the sn-2 position of TAG and to produce triricinolein (or castor oil) for industrial uses. The content of normal FA at the sn-2 position was about 95 %, mainly oleate (38 %), linolenate (31 %) and linoleate (23 %). This high normal FA content (95 %) at the sn-2 position was a big space for the replacement of ricinoleate to increase the hydroxy FA content in lesquerella oil. The content of hydroxy FA at the sn-1/3 positions was 91 % mainly lesquerolic acid (85 %) and the content of normal FA was 6.7 % at the sn-1/3 position in lesquerella oil. C1 [Lin, Jiann-Tsyh; Fagerquist, Clifton K.; Chen, Grace Q.] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Lin, JT (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM jiann.lin@ars.usda.gov NR 23 TC 2 Z9 2 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD FEB PY 2016 VL 93 IS 2 BP 183 EP 191 DI 10.1007/s11746-015-2769-2 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DB7NH UT WOS:000368701800003 ER PT J AU McKeon, TA Patfield, SA He, XH AF McKeon, Thomas A. Patfield, Stephanie A. He, Xiaohua TI Evaluation of Castor Oil Samples for Potential Toxin Contamination SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Toxin detection; Ricinus communis; Ricin; Ricinoleic acid; Lithium grease; Oil processing ID ALLERGENICITY AB Castor oil and its derivatives are widely used as a chemical feedstock for production of lubricants and greases, engineering plastics, plasticizers and surfactants. It also has wide application in consumer goods such as lipstick, deodorants and medicinal uses. Due to concerns about the possible presence of the ricin toxin in the oil, we have tested a collection of castor oils processed using different approaches, including cold-pressed, US Pharmaceutical (USP) grade, and neutralized oils. Water soluble proteins were extracted from oil samples into phosphate-buffered saline containing 0.05 % bovine serum albumin (PBSB) and analyzed for potential ricin contamination by ELISA. Our results indicate that only the cold-pressed castor oil contained measurable levels of the toxin, estimated to be 35 +/- 13 mu g/l. A normal oral dose of castor oil for laxative use is 14 ml, so even cold-pressed castor oil would be well below the toxic level of 1-5 mu g/kg body weight. However, the presence of the toxin indicates that other soluble proteins, including allergens, may be present in cold-pressed castor oil. C1 [McKeon, Thomas A.; Patfield, Stephanie A.; He, Xiaohua] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP He, XH (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM thomas.mckeon@ars.usda.gov; stephanie.patfield@ars.usda.gov; xiaohua.he@ars.usda.gov NR 11 TC 1 Z9 1 U1 7 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD FEB PY 2016 VL 93 IS 2 BP 299 EP 301 DI 10.1007/s11746-015-2766-5 PG 3 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DB7NH UT WOS:000368701800015 ER PT J AU Guo, L Breakspear, A Zhao, GY Gao, LX Kistler, HC Xu, JR Ma, LJ AF Guo, Li Breakspear, Andrew Zhao, Guoyi Gao, Lixin Kistler, H. Corby Xu, Jin-Rong Ma, Li-Jun TI Conservation and divergence of the cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) pathway in two plant-pathogenic fungi: Fusarium graminearum and F-verticillioides SO MOLECULAR PLANT PATHOLOGY LA English DT Article DE cAMP; cell signalling; Fusarium; orthologous; PKA; speciation; transcriptome ID SACCHAROMYCES-CEREVISIAE; MAGNAPORTHE-GRISEA; ADENYLATE-CYCLASE; SIGNAL-TRANSDUCTION; GENE-REGULATION; VIRULENCE; GROWTH; YEAST; BIOSYNTHESIS; METABOLISM AB The cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) pathway is a central signalling cascade that transmits extracellular stimuli and governs cell responses through the second messenger cAMP. The importance of cAMP signalling in fungal biology has been well documented and the key conserved components, adenylate cyclase (AC) and the catalytic subunit of PKA (CPKA), have been functionally characterized. However, other genes involved in this signalling pathway and their regulation are not well understood in filamentous fungi. Here, we performed a comparative transcriptomics analysis of AC and CPKA mutants in two closely related fungi: Fusarium graminearum (Fg) and F.verticillioides (Fv). Combining available Fg transcriptomics and phenomics data, we reconstructed the Fg cAMP signalling pathway. We developed a computational program that combines sequence conservation and patterns of orthologous gene expression to facilitate global transcriptomics comparisons between different organisms. We observed highly correlated expression patterns for most orthologues (80%) between Fg and Fv. We also identified a subset of 482 (6%) diverged orthologues, whose expression under all conditions was at least 50% higher in one genome than in the other. This enabled us to dissect the conserved and unique portions of the cAMP-PKA pathway. Although the conserved portions controlled essential functions, such as metabolism, the cell cycle, chromatin remodelling and the oxidative stress response, the diverged portions had species-specific roles, such as the production and detoxification of secondary metabolites unique to each species. The evolution of the cAMP-PKA signalling pathway seems to have contributed directly to fungal divergence and niche adaptation. C1 [Guo, Li; Ma, Li-Jun] Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA. [Breakspear, Andrew; Kistler, H. Corby] Univ Minnesota, Cereal Dis Lab, USDA ARS, St Paul, MN 55108 USA. [Zhao, Guoyi; Gao, Lixin] Univ Massachusetts, Dept Elect & Comp Engn, Amherst, MA 01003 USA. [Xu, Jin-Rong] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. RP Ma, LJ (reprint author), Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA. EM lijun@biochem.umass.edu OI Guo, Li/0000-0001-6100-3481 FU National Research Initiative Competitive Grants Program from USDA National Institute of Food and Agriculture [2008-35604-18800, MASR-2009-04374]; Massachusetts Green High Performance Computing Center (MGHPCC); National Research Initiative Hatch Grants Program [MAS00441] FX This project was supported by the National Research Initiative Competitive Grants Program Grant no. 2008-35604-18800 and MASR-2009-04374 from the USDA National Institute of Food and Agriculture. LG and L-JM are also supported by a seed grant from Massachusetts Green High Performance Computing Center (MGHPCC) and the National Research Initiative Hatch Grants Program Grant no. MAS00441. The authors would like to thank Dr Xiaoying Zhou for providing RNA samples for the microarray experiment. The authors would also like to thank the Broad expression platform for conducting hybridization of both DNA and RNA samples to the Affymetrix arrays and processing the microarray results. NR 52 TC 3 Z9 3 U1 5 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD FEB PY 2016 VL 17 IS 2 BP 196 EP 209 DI 10.1111/mpp.12272 PG 14 WC Plant Sciences SC Plant Sciences GA DB7OE UT WOS:000368704300006 PM 25907134 ER PT J AU Bingner, RL Wells, RR Momm, HG Rigby, JR Theurer, FD AF Bingner, R. L. Wells, R. R. Momm, H. G. Rigby, J. R. Theurer, F. D. TI Ephemeral gully channel width and erosion simulation technology SO NATURAL HAZARDS LA English DT Article DE Ephemeral gullies; Erosion; Sediment yield; Watersheds; Models ID MODEL; ANNAGNPS; PREDICTION AB Concentrated surface runoff, such as associated with ephemeral gully channels, increases erosion and transfers fine sediment and associated agrichemicals from upland areas to stream channels. Ephemeral gully erosion on cropland may contribute up to 40 % or more of the sediment delivered to the edge of agricultural fields, significantly threatening the health of downstream ecological services. Typically, conservation practices developed for sheet and rill erosion are also expected to treat ephemeral gully erosion, but technology and tools are needed to account for the separate benefits and effects of practices on various sediment sources. Without improved research studies, subjective observations will continue to be used to satisfy quality criteria in lieu of scientifically defensible, quantitative methods to estimate the impact of gully erosion. Ephemeral gully channels evolve by one, or combination of, complex physical process in the form of incision, headcut migration upslope, and channel sidewalls expansion (widening). This study focused on the latter, ephemeral gully channel widening relationships. The impact of various width functions on erosion can be very significant and is dependent on discharge, slope, soil properties, and management conditions. A description is provided on six ephemeral gully widening relationships, followed by recommended improvements, comparative application, and identification of future research needs. Improvements in the development of ephemeral gully width algorithms are critical to understanding the impact of conservation practices on controlling ephemeral gully erosion. Tools are needed to predict and quantify ephemeral gully erosion, including the capability to evaluate the effect of conservation practices to control erosion. An improved critical shear stress equation was developed and described that provides an approach to incorporating impacts of management practices on the resulting gully erodibility. Conservation management planning by organizations needs a systematic approach to determining the extent of ephemeral gully erosion problems on a field, watershed, or national basis, or to predict the recurring or new locations of ephemeral gullies prior to their development. C1 [Bingner, R. L.; Wells, R. R.; Rigby, J. R.] USDA ARS, Natl Sedimentat Lab, POB 1157, Oxford, MS 38655 USA. [Momm, H. G.] Middle Tennessee State Univ, Dept Geosci, Kirksey Old Main 322G, Murfreesboro, TN 37132 USA. [Theurer, F. D.] USDA NRCS, Gaithersburg, MD 20879 USA. RP Bingner, RL (reprint author), USDA ARS, Natl Sedimentat Lab, POB 1157, Oxford, MS 38655 USA. EM Ron.Bingner@ars.usda.gov; Robert.Wells@ars.usda.gov; Henrique.Momm@mtsu.edu; JR.Rigby@ars.usda.gov; Fred.Theurer@verizon.net NR 34 TC 0 Z9 0 U1 13 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0921-030X EI 1573-0840 J9 NAT HAZARDS JI Nat. Hazards PD FEB PY 2016 VL 80 IS 3 BP 1949 EP 1966 DI 10.1007/s11069-015-2053-7 PG 18 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Water Resources SC Geology; Meteorology & Atmospheric Sciences; Water Resources GA DB6QP UT WOS:000368640300025 ER PT J AU Rubio, YLB Duarte, JLG Bates, RO Ernst, CW Nonneman, D Rohrer, GA King, A Shackelford, SD Wheeler, TL Cantet, RJC Steibel, JP AF Bernal Rubio, Y. L. Gualdron Duarte, J. L. Bates, R. O. Ernst, C. W. Nonneman, D. Rohrer, G. A. King, A. Shackelford, S. D. Wheeler, T. L. Cantet, R. J. C. Steibel, J. P. TI Meta-analysis of genome-wide association from genomic prediction models SO ANIMAL GENETICS LA English DT Article DE GBLUP; genome-wide association studies; multiple populations ID QUANTITATIVE TRAIT LOCI; MEAT QUALITY TRAITS; MAPPING INCLUDING PHENOTYPES; JOINT ANALYSIS; BEEF-CATTLE; POPULATION STRATIFICATION; IMPUTATION ACCURACY; GENETIC EVALUATION; BREEDING VALUES; PIG-POPULATION AB Genome-wide association (GWA) studies based on GBLUP models are a common practice in animal breeding. However, effect sizes of GWA tests are small, requiring larger sample sizes to enhance power of detection of rare variants. Because of difficulties in increasing sample size in animal populations, one alternative is to implement a meta-analysis (MA), combining information and results from independent GWA studies. Although this methodology has been used widely in human genetics, implementation in animal breeding has been limited. Thus, we present methods to implement a MA of GWA, describing the proper approach to compute weights derived from multiple genomic evaluations based on animal-centric GBLUP models. Application to real datasets shows that MA increases power of detection of associations in comparison with population-level GWA, allowing for population structure and heterogeneity of variance components across populations to be accounted for. Another advantage of MA is that it does not require access to genotype data that is required for a joint analysis. Scripts related to the implementation of this approach, which consider the strength of association as well as the sign, are distributed and thus account for heterogeneity in association phase between QTL and SNPs. Thus, MA of GWA is an attractive alternative to summarizing results from multiple genomic studies, avoiding restrictions with genotype data sharing, definition of fixed effects and different scales of measurement of evaluated traits. C1 [Bernal Rubio, Y. L.; Gualdron Duarte, J. L.; Bates, R. O.; Ernst, C. W.; Steibel, J. P.] Univ Buenos Aires, Fac Agron, Dept Anim Prod, RA-1417 Buenos Aires, DF, Argentina. [Bernal Rubio, Y. L.; Cantet, R. J. C.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Nonneman, D.; Rohrer, G. A.; King, A.; Shackelford, S. D.; Wheeler, T. L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Cantet, R. J. C.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Steibel, J. P.] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI 48824 USA. RP Steibel, JP (reprint author), Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. EM steibelj@msu.edu OI Cantet, Rodolfo/0000-0001-6282-146X FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2010-65205-20342]; National Pork Board Grant [11-042]; Pork Checkoff (National Pork Board); US Pig Genome Coordination Funds; MSU Animal Agriculture Initiative; Administrative Department of Science, Technology and Innovation COLCIENCIAS (Colombia) FX This work was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010-65205-20342 from the USDA National Institute of Food and Agriculture and by funding from National Pork Board Grant no. 11-042. Phenotypic data collection of the commercial population was funded partially by the Pork Checkoff (National Pork Board). Furthermore, partial funding was provided by US Pig Genome Coordination Funds and the MSU Animal Agriculture Initiative and also by the Administrative Department of Science, Technology and Innovation COLCIENCIAS (Colombia). Computer resources were provided by the Michigan State University High Performance Computing Center. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. USDA prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs). Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). NR 78 TC 3 Z9 3 U1 6 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2016 VL 47 IS 1 BP 36 EP 48 DI 10.1111/age.12378 PG 13 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA DB0BH UT WOS:000368173100005 ER PT J AU Ulyshen, MD AF Ulyshen, Michael D. TI Wood decomposition as influenced by invertebrates SO BIOLOGICAL REVIEWS LA English DT Article DE arthropods; biodiversity; ecosystem services; insects; Isoptera; saproxylic ID FUNGUS-GROWING TERMITES; TROPICAL RAIN-FOREST; CORD-FORMING FUNGI; NITROGEN-FIXATION; LITTER DECOMPOSITION; CELLULOSE DIGESTION; DECAYING WOOD; COLEOPTERA CERAMBYCIDAE; NUTRIENT CONCENTRATIONS; TERRESTRIAL ECOSYSTEMS AB The diversity and habitat requirements of invertebrates associated with dead wood have been the subjects of hundreds of studies in recent years but we still know very little about the ecological or economic importance of these organisms. The purpose of this review is to examine whether, how and to what extent invertebrates affect wood decomposition in terrestrial ecosystems. Three broad conclusions can be reached from the available literature. First, wood decomposition is largely driven by microbial activity but invertebrates also play a significant role in both temperate and tropical environments. Primary mechanisms include enzymatic digestion (involving both endogenous enzymes and those produced by endo- and ectosymbionts), substrate alteration (tunnelling and fragmentation), biotic interactions and nitrogen fertilization (i.e. promoting nitrogen fixation by endosymbiotic and free-living bacteria). Second, the effects of individual invertebrate taxa or functional groups can be accelerative or inhibitory but the cumulative effect of the entire community is generally to accelerate wood decomposition, at least during the early stages of the process (most studies are limited to the first 2-3years). Although methodological differences and design limitations preclude meta-analysis, studies aimed at quantifying the contributions of invertebrates to wood decomposition commonly attribute 10-20% of wood loss to these organisms. Finally, some taxa appear to be particularly influential with respect to promoting wood decomposition. These include large wood-boring beetles (Coleoptera) and termites (Termitoidae), especially fungus-farming macrotermitines. The presence or absence of these species may be more consequential than species richness and the influence of invertebrates is likely to vary biogeographically. C1 [Ulyshen, Michael D.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. EM mulyshen@fs.fed.us NR 192 TC 13 Z9 13 U1 30 U2 98 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-7931 EI 1469-185X J9 BIOL REV JI Biol. Rev. PD FEB PY 2016 VL 91 IS 1 BP 70 EP 85 DI 10.1111/brv.12158 PG 16 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DA7LI UT WOS:000367985200004 PM 25424353 ER PT J AU Wang, ZY Han, LF Sun, K Jin, J Ro, KS Libra, JA Liu, XT Xing, BS AF Wang, Ziying Han, Lanfang Sun, Ke Jin, Jie Ro, Kyoung S. Libra, Judy A. Liu, Xitao Xing, Baoshan TI Sorption of four hydrophobic organic contaminants by biochars derived from maize straw, wood dust and swine manure at different pyrolytic temperatures SO CHEMOSPHERE LA English DT Article DE Biochar; CO2-surface area; Surface polarity; Sorption; Minerals ID STRUCTURAL-PROPERTIES; MOLECULAR-STRUCTURE; PHENANTHRENE; MATTER; CARBONIZATION; MECHANISMS; ADSORPTION; IMPACT; POLAR; CHARS AB Sorption behavior of acetochlor (ACE), dibutyl phthalate (DBP), 17 alpha-Ethynyl estradiol (EE2) and phenanthrene (PHE) with biochars produced from three feedstocks (maize straw (MABs), pine wood dust (WDBs) and swine manure (SWBs)) at seven heat treatment temperatures (HTTs) was evaluated. The bulk polarity of these biochars declined with increasing HIT while the aromaticity and CO2-surface area (CO2-SA) rose. The surface OC contents of biochars were generally higher than bulk OC contents. The organic carbon (OC)-normalized CO2-SA (CO2-SA/OC) of biochars significantly correlated with the sorption coefficients (n and logK(oc)), suggesting that pore filling could dominate the sorption of tested sorbates. SWBs had higher logK(oc) values compared to MABs and WDBs, due to their higher ash contents. Additionally, the logK(oc) values for MABs was relatively greater than that for WDBs at low HTTs (<= 400 degrees C), probably resulting from the higher CO2-SA/OC, ash contents and aromaticity of MABs. Surface polarity and the aliphatic C may dominate the sorption of WDBs obtained at relatively low HTFs (<= 400 degrees C), while aromatic C affects the sorption of biochars at high HTFs. Results of this work aid to deepen our understanding of the sorption mechanisms, which is pivotal to wise utilization of biochars as sorbents for hazardous organic compounds. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Wang, Ziying; Han, Lanfang; Sun, Ke; Jin, Jie; Liu, Xitao] Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China. [Ro, Kyoung S.] ARS, Coastal Plains Soil Water & Plant Res Ctr, USDA, Florence, SC 29501 USA. [Libra, Judy A.] Leibniz Inst Agr Engn, D-14469 Potsdam, Germany. [Xing, Baoshan] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. RP Sun, K (reprint author), Beijing Normal Univ, Sch Environm, State Key Lab Water Environm Simulat, Beijing 100875, Peoples R China. EM sunke@bnu.edu.cn RI Libra, Judy/K-3092-2013 OI Libra, Judy/0000-0001-9307-7776 FU Fund for Innovative Research Group of the National Natural Science Foundation of China [51421065]; National Natural Science Foundation of China [41273106]; USDA Hatch Program [MAS 00982]; Beijing Higher Education Young Elite Teacher Project; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry FX This research was supported by the Fund for Innovative Research Group of the National Natural Science Foundation of China (51421065), National Natural Science Foundation of China (41273106), USDA Hatch Program (MAS 00982), Beijing Higher Education Young Elite Teacher Project, and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 26 TC 3 Z9 3 U1 26 U2 93 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD FEB PY 2016 VL 144 BP 285 EP 291 DI 10.1016/j.chemosphere.2015.08.042 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DA4MQ UT WOS:000367774400038 PM 26364218 ER PT J AU Devi, MJ Sinclair, TR Taliercio, E AF Devi, M. Jyostna Sinclair, Thomas R. Taliercio, Earl TI Silver and zinc inhibitors influence transpiration rate and aquaporin transcript abundance in intact soybean plants SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Aquaporins; Silver; Soybean; Transpiration; Vapor pressure deficit; Zinc ID TIME RT-PCR; DROUGHT TOLERANCE; GENE-EXPRESSION; PI 471938; GENOTYPES; BENEFITS; CHANNELS; CELLS AB Soybean genotype PI 416937 has previously been found to have low leaf hydraulic conductivity, which was hypothesized to be the basis for limited transpiration (TRlim) rates under high vapor pressure deficit. Previous studies with de-rooted shoots showed there was a consistency in the expression of TRlim, and plant response to exposure to silver, an aquaporin (AQP) inhibitor. However, it is not clear what confounding influence treatments with metal AQP inhibitors may have on AQP transcript expression. This study was undertaken to extend the observations of response to silver and zinc using intact plants. In a comparison of four genotypes, intact plants of PI 416937 were uniquely insensitive to exposure to silver and of PI 471938 were uniquely insensitive to zinc. RNA abundance of eight AQPs was measured after treatment with the AQP inhibitors. There were differences in the abundance of RNA among genotypes. There was a general trend of less change in abundance in PI 416937 following silver treatment in contrast to increased abundance in the other genotypes. This result would be consistent with little or no response in transpiration rate in the intact plants of PI 416937 following silver treatment. Following zinc treatment, there was a rapid increase in RNA abundance in PI 471938 in comparison to the other genotypes. However, the changes in AQP abundance following treatment with metal inhibitors indicates the likely interaction of direct response of AQP to metals and alternation of expression of AQP transcripts. These results indicate that the results from treatment with metal inhibitors should likely be considered only as preliminary screens from which genotypes need to be tested directly for the TRlim trait. (C) 2015 Elsevier B.V. All rights reserved. C1 [Devi, M. Jyostna; Sinclair, Thomas R.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Taliercio, Earl] USDA ARS, Soybean & Nitrogen Fixat Unit, Raleigh, NC 27695 USA. RP Sinclair, TR (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM trsincla@ncsu.edu FU United Soybean Board [1213] FX The authors gratefully acknowledge the funding support of the United Soybean Board, Project 1213. NR 28 TC 1 Z9 1 U1 8 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD FEB PY 2016 VL 122 BP 168 EP 175 DI 10.1016/j.envexpbot.2015.10.006 PG 8 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA DB0TD UT WOS:000368219500020 ER PT J AU Seidl, R Spies, TA Peterson, DL Stephens, SL Hicke, JA AF Seidl, Rupert Spies, Thomas A. Peterson, David L. Stephens, Scott L. Hicke, Jeffrey A. TI Searching for resilience: addressing the impacts of changing disturbance regimes on forest ecosystem services SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE climate change impacts; ecological resilience; ecosystem services; engineering resilience; forest ecosystem management; natural disturbance; range of variability; socio-ecological resilience ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; CLIMATE-CHANGE; BARK BEETLES; FIRE REGIMES; MANAGEMENT; DYNAMICS; INCREASE; WILDFIRE; EUROPE AB The provisioning of ecosystem services to society is increasingly under pressure from global change. Changing disturbance regimes are of particular concern in this context due to their high potential impact on ecosystem structure, function and composition. Resilience-based stewardship is advocated to address these changes in ecosystem management, but its operational implementation has remained challenging. We review observed and expected changes in disturbance regimes and their potential impacts on provisioning, regulating, cultural and supporting ecosystem services, concentrating on temperate and boreal forests. Subsequently, we focus on resilience as a powerful concept to quantify and address these changes and their impacts, and present an approach towards its operational application using established methods from disturbance ecology. We suggest using the range of variability concept - characterizing and bounding the long-term behaviour of ecosystems - to locate and delineate the basins of attraction of a system. System recovery in relation to its range of variability can be used to measure resilience of ecosystems, allowing inferences on both engineering resilience (recovery rate) and monitoring for regime shifts (directionality of recovery trajectory). It is important to consider the dynamic nature of these properties in ecosystem analysis and management decision-making, as both disturbance processes and mechanisms of resilience will be subject to changes in the future. Furthermore, because ecosystem services are at the interface between natural and human systems, the social dimension of resilience (social adaptive capacity and range of variability) requires consideration in responding to changing disturbance regimes in forests.Synthesis and applications. Based on examples from temperate and boreal forests we synthesize principles and pathways for fostering resilience to changing disturbance regimes in ecosystem management. We conclude that future work should focus on testing and implementing these pathways in different contexts to make ecosystem services provisioning more robust to changing disturbance regimes and advance our understanding of how to cope with change and uncertainty in ecosystem management. C1 [Seidl, Rupert] Univ Nat Resources & Life Sci BOKU, Dept Forest & Soil Sci, Inst Silviculture, A-1190 Vienna, Austria. [Spies, Thomas A.] USDA Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Peterson, David L.] USDA Forest Serv, Pacific NW Res Stn, Seattle, WA 98103 USA. [Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Ecosyst Sci Div, Berkeley, CA 94720 USA. [Hicke, Jeffrey A.] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. RP Seidl, R (reprint author), Univ Nat Resources & Life Sci BOKU, Dept Forest & Soil Sci, Inst Silviculture, Peter Jordan Str 82, A-1190 Vienna, Austria. EM rupert.seidl@boku.ac.at OI Seidl, Rupert/0000-0002-3338-3402 FU Austrian Science Fund [P25503-B16]; Marie Curie Career Integration Grant of the European Community [PCIG12-GA-2012 334104] FX R. Seidl acknowledges support from the Austrian Science Fund (P25503-B16) as well as from a Marie Curie Career Integration Grant of the European Community (PCIG12-GA-2012 334104). This paper is the result of a session held at the XXIV IUFRO World Congress in Salt Lake City, UT, USA, on 9 October 2014. We thank M.-J. Schelhaas and two anonymous reviewers for helpful comments on earlier versions of the manuscript. NR 67 TC 11 Z9 11 U1 32 U2 127 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD FEB PY 2016 VL 53 IS 1 BP 120 EP 129 DI 10.1111/1365-2664.12511 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DA8WU UT WOS:000368088300015 PM 26966320 ER PT J AU Halpern, CB Antos, JA McKenzie, D Olson, AM AF Halpern, Charles B. Antos, Joseph A. McKenzie, Donald Olson, Annette M. TI Past tree influence and prescribed fire mediate bioticinteractions and community reassembly in agrassland-restoration experiment SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE burning; competition; inhibition; meadow restoration; pre-emption; priority effects; species' interactions; tree encroachment ID MONTANE MEADOW; GRASSLAND-RESTORATION; ECOSYSTEM MANAGEMENT; CONIFER ENCROACHMENT; WOODY ENCROACHMENT; POSITIVE FEEDBACKS; TALLGRASS PRAIRIE; UNDERSTORY LAYERS; MESIC GRASSLAND; CASCADE RANGE AB Woody plant encroachment of grasslands is occurring globally, with profound ecological consequences. Attempts to restore herbaceous dominance may fail if the woody state is resilient or if intervention leads to an alternate, undesirable state. Restoration outcomes often hinge on biotic interactions - particularly on priority effects that inhibit or promote community reassembly. Following experimental tree removal from conifer-invaded grasslands, we documented substantial variation in community reassembly associated with the changing abundance of the native clonal sedge Carex inops L.H. Bailey subsp. inops. We explored possible mechanisms for this variation, focusing on the nature and timing of interactions between the meadow community and Carex and on how past tree influence and prescribed fire mediate the outcomes of these interactions. Meadow species increased after tree removal, but less so in burned than in unburned plots. Carex expanded dramatically after fire, particularly where past tree influence had been greater. Meadow species and Carex developed an increasingly negative association over time; pre-emption was reciprocal, but offset in time and space. Meadow species inhibited Carex through vegetative recovery in areas of limited or recent tree influence, irrespective of fire. Carex inhibited meadow reassembly in areas of greater tree influence, but only with burning.Synthesis and applications. Tree removal and fire imposed across a range of altered meadow states yielded varying outcomes, reflecting biotic interactions and species' regenerative traits that inhibited or promoted reassembly. Fire tended to destabilize the remnant meadow community and, in areas more degraded by encroachment, stimulated release of Carex, which inhibited reassembly. Knowledge of the context dependence of biotic interactions can enhance the effectiveness of restoration by establishing the bounds within which treatments produce desirable or undesirable outcomes. C1 [Halpern, Charles B.; McKenzie, Donald] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Antos, Joseph A.] Univ Victoria, Dept Biol, Victoria, BC V8W 2Y2, Canada. [McKenzie, Donald] USDA Forest Serv, Pacific Wildland Fire Sci Lab, Seattle, WA USA. [Olson, Annette M.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. RP Halpern, CB (reprint author), Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. EM chalpern@uw.edu FU Joint Fire Science Program; Willamette National Forest; Rocky Mountain Elk Foundation FX We thank our many field assistants. Cheryl Friesen and staff and fire crews from the McKenzie River Ranger District and Confederated Tribes of the Grand Ronde provided logistical support. Three referees provided suggestions to improve the manuscript. Funding was provided by the Joint Fire Science Program, Willamette National Forest, and Rocky Mountain Elk Foundation. NR 58 TC 2 Z9 2 U1 3 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD FEB PY 2016 VL 53 IS 1 BP 264 EP 273 DI 10.1111/1365-2664.12570 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DA8WU UT WOS:000368088300029 ER PT J AU Klick, J Yang, WQ Walton, VM Dalton, DT Hagler, JR Dreves, AJ Lee, JC Bruck, DJ AF Klick, J. Yang, W. Q. Walton, V. M. Dalton, D. T. Hagler, J. R. Dreves, A. J. Lee, J. C. Bruck, D. J. TI Distribution and activity of Drosophila suzukii in cultivated raspberry and surrounding vegetation SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Drosophilidae; immunomarking; insect dispersal; non-crop host; Rubus armeniacus; spatial statistics; Spotted wing drosophila ID SPOTTED-WING DROSOPHILA; LONG-DISTANCE MIGRATION; FRUIT-FLY DIPTERA; PEST-MANAGEMENT; SPATIAL-PATTERNS; HEMIPTERA CICADELLIDAE; ORGANIC STRAWBERRIES; POPULATION-STRUCTURE; UNITED-STATES; FLIES DIPTERA AB Spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), may utilize wild 'Himalaya' blackberry (HB) Rubus armeniacus Focke or other non-crop plants as refugia and possibly exploit adjacent field margins before colonizing cultivated fruiting crops. Studies were conducted to determine the role of field margins containing HB and their effect on D. suzukii activity, density and distribution in an adjacent commercial red raspberry crop. One-ha plots adjacent to field margins containing HB or known non-host (NH) grass crops were established in 2011 and 2012 and replicated three times. Each plot contained two transects with monitoring traps for D. suzukii in the field margin (0 m) and spaced approximately 10 (crop boundary), 40, 70 and 100 m into the adjacent crop (n = 10 traps/plot). Field margin vegetation was treated with a 10% chicken egg white mark solution weekly from pre-harvest until the end of harvest using a cannon sprayer. Adult D. suzukii were collected from traps weekly and analysed for the presence of the egg white mark using an egg white-specific enzyme-linked immunosorbent assay (ELISA). During both years, marked flies and total flies were captured in higher numbers in HB field margins, whereas virtually no flies were captured in field margins containing no known alternative host. Similarly, more flies were captured in the crop near HB than near NH. Spatial Analysis by Distance IndicEs (SADIE) and mean D. suzukii trap captures additionally displayed significantly higher fly densities in the raspberry field near HB than near NH. These results suggest that HB may contribute to elevated D. suzukii populations and pest pressure in comparison with field margins containing no known alternate host vegetation for D. suzukii. Having closely adjacent non-crop alternate host landscapes may result in increased D. suzukii pest pressure. C1 [Klick, J.; Walton, V. M.; Dalton, D. T.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Klick, J.; Lee, J. C.; Bruck, D. J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR USA. [Yang, W. Q.] Oregon State Univ, North Willamette Res & Extens Ctr, Aurora, OR USA. [Hagler, J. R.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Dreves, A. J.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. RP Klick, J (reprint author), Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. EM jimmyklick@gmail.com FU Oregon State University Agricultural Research Foundation; USD SACRI [2010-51181-21167]; USDA CRIS [5358-22000-037-00D] FX We acknowledge Adam Cave, Kelly Donahue, Joe Kleiber, Amanda Lake, Scott Machtley, Scott Shane, Bev Thomas and Amelia Thornhill for their diligent technical support and the cooperating raspberry grower for his collaboration and patience during the busy field season. Thanks to Oregon State University Statistics Student Consulting Service, Laura Sims and Megan Woltz for statistical assistance. Funding was provided by Oregon State University Agricultural Research Foundation, USD SACRI Grant 2010-51181-21167 and USDA CRIS 5358-22000-037-00D. Mention of proprietary or brand names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval to the exclusion of others that also may be suitable. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of source. NR 71 TC 16 Z9 16 U1 29 U2 78 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2016 VL 140 IS 1-2 BP 37 EP 46 DI 10.1111/jen.12234 PG 10 WC Entomology SC Entomology GA DA9QP UT WOS:000368144900005 ER PT J AU Tochen, S Woltz, JM Dalton, DT Lee, JC Wiman, NG Walton, VM AF Tochen, S. Woltz, J. M. Dalton, D. T. Lee, J. C. Wiman, N. G. Walton, V. M. TI Humidity affects populations of Drosophila suzukii (Diptera: Drosophilidae) in blueberry SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE fecundity; longevity; ovarian maturation; relative humidity; spotted wing drosophila ID SPOTTED-WING DROSOPHILA; PRE-ADULT DEVELOPMENT; RELATIVE-HUMIDITY; UNITED-STATES; WATER-LOSS; TEMPERATURE; MELANOGASTER; TEPHRITIDAE; RESISTANCE; RESPONSES AB Temperature and humidity affect insect physiology, survival, fecundity, reproductive status and behaviour. Complementing previous work investigating the effects of temperature on adult survival and fecundity of the invasive frugivorous pest, Drosophila suzukii (Matsumura), this study was conducted to determine the effect of humidity on D. suzukii larval development, adult survival, fecundity and reproductive status using blueberry as a host substrate. The five constant humidity levels in laboratory bioassays were 20, 33, 71, 82 and 94% RH at 20.6 +/- 0.2 degrees C. As RH increased, fecundity and longevity increased. At the higher humidity levels, RH had limited impact on mean generation times (T), larval development and eclosion times. The highest net reproductive rate (R-0 = 68) and highest intrinsic rate of population increase (r(m) = 0.17) were both recorded at 94% RH. The reproductive status of females, as indicated by the number of mature oocytes per female, was significantly greater at 82 and 94% RH, compared to 71% RH. In addition to the laboratory procedures, we correlated field trap captures over an 81-day summer period to relative humidity (RH) levels in close proximity to those traps. In the field, low ambient humidity levels resulted in decreased trap captures. A humidity-dependent population model predicted lower densities of D. suzukii relative to populations at higher humidity. This study supports the hypothesis that cultural practices that minimize lower humidity levels in crops can contribute to the management of D. suzukii. Such methods may include open pruning, drip irrigation and field floor management. C1 [Tochen, S.; Woltz, J. M.; Dalton, D. T.; Wiman, N. G.; Walton, V. M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Lee, J. C.] USDA ARS, Hort Crops Res Unit, Corvallis, OR USA. RP Dalton, DT (reprint author), Oregon State Univ, Dept Hort, 4017 ALS, Corvallis, OR 97331 USA. EM daniel.dalton@oregonstate.edu FU U.S. Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) award [2010-51181-21167]; CRIS [5358-2-22000-037-00D] FX The authors extend appreciation to the growers who allowed access for field trials. Funding for this research was provided from the U.S. Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) award #2010-51181-21167, and CRIS 5358-2-22000-037-00D. Technical assistance in the field and laboratory was provided by Riki York. NR 48 TC 9 Z9 9 U1 12 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2016 VL 140 IS 1-2 BP 47 EP 57 DI 10.1111/jen.12247 PG 11 WC Entomology SC Entomology GA DA9QP UT WOS:000368144900006 ER PT J AU Tomaseto, AF Krugner, R Lopes, JRS AF Tomaseto, A. F. Krugner, R. Lopes, J. R. S. TI Effect of plant barriers and citrus leaf age on dispersal of Diaphorina citri (Hemiptera: Liviidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE asian citrus psyllid; host plant phenology; huanglongbing; insect vector management; mark-release-recapture; trap plants ID ASIAN CITRUS; HOMALODISCA-COAGULATA; GREENING DISEASE; HOST PLANTS; PSYLLIDAE; CICADELLIDAE; HOMOPTERA; FLORIDA; MANAGEMENT; KUWAYAMA AB Studies designed to measure dispersal capacity of Diaphorina citri Kuwayama (Hemiptera: Liviidae) are needed to provide the epidemiological knowledge necessary to improve management of citrus huanglongbing. In this study, a mark-release-recapture technique was used to investigate whether 1) host or non-host plants of D. citri can act as barriers for dispersing insects and 2) presence or absence of young citrus leaves influence movement of D. citri towards citrus plants. The experimental field consisted of four circular and adjacent areas containing citrus trees, Citrus sinensis (L.) Osbeck cv. 'Hamlin', planted in concentric circles at 18, 24 and 30 m from the release centre. Insect activity was monitored by recapturing at each distance using yellow stick traps. Dense plantings of tall non-host plants of D. citri such as corn had no effect on insect dispersal towards citrus plants when compared to a shorter cover crop such as grass. In contrast, suitable host plants acted as traps decreasing movement of D. citri. Diaphorina citri dispersed at greater speeds in the absence of young leaves reaching 140 m within 6 hours after release, whereas in the presence of young leaves, individuals reached at most 60 m at 1 day after release. Results suggest that D. citri control measures may be more efficient during periods of highest vegetative activity when insects are less active. Moreover, the use of suitable host plants for D. citri as trap plants may be a potential tactic to prevent movement of insects into the crop. C1 [Tomaseto, A. F.; Lopes, J. R. S.] Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP, Brazil. [Krugner, R.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. RP Tomaseto, AF (reprint author), Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, Av Padua Dias 11, BR-13418900 Piracicaba, SP, Brazil. EM arthur.tomaseto@usp.br RI Lopes, Joao/F-3439-2012 OI Lopes, Joao/0000-0002-9998-5473 FU National Council for Scientific and Technological Development (CNPq)/Brazil [309883/2011-3, 133236/2011-0]; Fundo de Defesa da Citricultura (Fundecitrus) FX This research was supported by Fundo de Defesa da Citricultura (Fundecitrus). We thank Grupo de Pesquisa e Extensao em Topografia e Geoprocessamento (TopoGeo) and Prof. P.R. Fiorio for the topographic survey; Prof. S.S. Neto, Prof. W.A.C. Godoy, Prof. R.A. Sermarini and Prof. S.M.S. Piedade for their help with statistical analyses; the Laboratory of Insect Biology from Department of Entomology and Acarology at ESALQ-USP for providing additional insects for experiments; and G.M. Passos for providing technical assistance. First and last author received scholarships from National Council for Scientific and Technological Development (CNPq)/Brazil (Proc. 309883/2011-3 and 133236/2011-0). This article is part of the first author's Master Thesis. NR 43 TC 3 Z9 3 U1 5 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2016 VL 140 IS 1-2 BP 91 EP 102 DI 10.1111/jen.12249 PG 12 WC Entomology SC Entomology GA DA9QP UT WOS:000368144900010 ER PT J AU Savage, J Ribaudo, M AF Savage, Jeff Ribaudo, Marc TI Improving the Efficiency of Voluntary Water Quality Conservation Programs SO LAND ECONOMICS LA English DT Article ID CARBON SEQUESTRATION; CHESAPEAKE BAY; UNITED-STATES; POLLUTION; SOIL; PRECIPITATION; AGRICULTURE; POLICIES; DESIGN; OPPORTUNITIES AB Voluntary approaches have traditionally been used to address environmental externalities emanating from agricultural production in the United States. However, voluntary approaches have largely failed to improve water quality in impaired waters. This paper assesses how to increase the efficiency of voluntary conservation in the context of the Chesapeake Bay. Field-level data representative of cropland in the bay watershed are analyzed using a programming model to quantify the gains of targeting technology-based incentives and of performance-based incentives. Performance-based approaches were the most efficient. The efficiency of technology-based approaches was improved by targeting cropland with features indicative of low marginal abatement costs. C1 [Savage, Jeff] USDA, Serv Agcy, Washington, DC 20250 USA. [Ribaudo, Marc] USDA, Econ Res Serv, Washington, DC 20250 USA. RP Savage, J (reprint author), USDA, Serv Agcy, Washington, DC 20250 USA. NR 53 TC 0 Z9 0 U1 3 U2 16 PU UNIV WISCONSIN PRESS PI MADISON PA JOURNAL DIVISION, 1930 MONROE ST, 3RD FL, MADISON, WI 53711 USA SN 0023-7639 EI 1543-8325 J9 LAND ECON JI Land Econ. PD FEB PY 2016 VL 92 IS 1 BP 148 EP 166 PG 19 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA DB2EN UT WOS:000368321200008 ER PT J AU Thiessen, LD Keune, JA Neill, TM Turechek, WW Grove, GG Mahaffee, WF AF Thiessen, L. D. Keune, J. A. Neill, T. M. Turechek, W. W. Grove, G. G. Mahaffee, W. F. TI Development of a grower-conducted inoculum detection assay for management of grape powdery mildew SO PLANT PATHOLOGY LA English DT Article DE Erysiphe necator; grapevine; powdery mildew; Vitis vinifera ID MEDIATED ISOTHERMAL AMPLIFICATION; POLYMERASE-CHAIN-REACTION; LATENT CLASS ANALYSIS; BOTRYTIS LEAF-BLIGHT; REAL-TIME PCR; ERYSIPHE-NECATOR; AIRBORNE INOCULUM; PSEUDOPERONOSPORA-HUMULI; DIAGNOSTIC-TESTS; RAPID DETECTION AB Management of grape powdery mildew (Erysiphe necator) and other polycyclic diseases often relies on calendar-based pesticide application schedules that assume the presence of inoculum. An inexpensive, loop-mediated isothermal amplification (LAMP) assay was designed to quickly detect airborne inoculum of E.necator to determine when to initiate a fungicide application programme. Field efficacy was tested in 2010 and 2011 in several commercial and research vineyards in the Willamette Valley of Oregon from pre-bud break to veraison. In each vineyard, three impaction spore traps were placed adjacent to the trunk. One trap was maintained and used by the grower to conduct the LAMP assay (G-LAMP) on-site and the other two traps were used for laboratory-conducted LAMP (L-LAMP) and quantitative PCR assay (qPCR). Using the qPCR as a gold standard, L-LAMP was comparable with qPCR in both years, and G-LAMP was comparable to qPCR in 2011. Latent class analysis indicated that qPCR had a true positive proportion of 98% in 2010 and 89% in 2011 and true negative proportion of 96% in 2010 and 64% in 2011. An average of 33 fewer fungicide applications were used when they were initiated based on spore detection relative to the grower standard practice. There were no significant differences in berry or leaf incidence between plots with fungicides initiated at detection or grower standard practice plots, suggesting that growers using LAMP to initiate fungicide applications can use fewer fungicide applications to manage powdery mildew compared to standard practices. C1 [Thiessen, L. D.; Mahaffee, W. F.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Keune, J. A.; Neill, T. M.; Mahaffee, W. F.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Turechek, W. W.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Grove, G. G.] Washington State Univ, Dept Plant Pathol, Prosser, WA 99350 USA. [Grove, G. G.] IRAC Prosser, Prosser, WA 99350 USA. RP Mahaffee, WF (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM walt.mahaffee@ars.usda.gov FU American Vineyard Foundation; Oregon Wine Board; Viticulture Consortium West; USDA-ARS CRIS [5358-22000-039-00D] FX This work was supported by American Vineyard Foundation, Oregon Wine Board, Viticulture Consortium West and USDA-ARS CRIS 5358-22000-039-00D. The authors thank the technical support of Andy Albrecht, Cole Provence and Jim Eynard. They acknowledge Marin Talbot Brewer and Michael Milgroom for use of ITS sequence data ahead of publication. The authors also thank anonymous reviewers for their helpful suggestions to improve the manuscript. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 52 TC 7 Z9 7 U1 8 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD FEB PY 2016 VL 65 IS 2 BP 238 EP 249 DI 10.1111/ppa.12421 PG 12 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DA7AJ UT WOS:000367956700008 ER PT J AU Bastos, MDR Laurentino, LD Canuto, KM Mendes, LG Martins, CM Silva, SMF Furtado, RF Kim, S Biswas, A Cheng, HN AF Bastos, Maria do Socorro Rocha Laurentino, Larissa da Silva Canuto, Kirley Marques Mendes, Luana Guabiraba Martins, Camila Mota Frota Silva, Sarah Maria Furtado, Roselayne Ferro Kim, Sanghoon Biswas, Atanu Cheng, H. N. TI Physical and mechanical testing of essential oil-embedded cellulose ester films SO POLYMER TESTING LA English DT Article DE Food packaging; Essential oil; Cellulose acetate; Cellulose acetate propionate; Cellulose acetate butyrate ID PLANT ESSENTIAL OILS; ANTIMICROBIAL PROPERTIES; CHEMICAL-COMPOSITION; BARRIER PROPERTIES; EDIBLE FILMS; ACETATE; PERMEABILITY; SURFACTANTS; SKIN AB Polymer films made from cellulose esters are useful for embedding plant essential oils, either for food packaging or air freshener applications. Studies and testing were done on the physical and mechanical properties of cellulose ester-based films incorporating essential oils (EO) from lemongrass (Cybopogon citratus), rosemary pepper (Lippia sidoides) and basil (Ocimum gratissimum) at concentrations of 10 and 20% (v/w). Results obtained showed that, in all films, the addition of the essential oil caused a decrease in the water vapor permeability due to the hydrophobic nature of the oil. The use of 20% of EO caused lower transparency of the films, although the change was not observed visually. Mechanical testing was done on cellulose acetate, cellulose acetate propionate and cellulose acetate butyrate. It was found that incorporation of lemongrass, basil and rosemary pepper EO significantly affected the Young's modulus, tensile strength and elongation at break of the cellulose ester films. The results suggested that the essential oils interacted with the polymers like plasticizers. The results were confirmed with thermal and microscopic studies. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Bastos, Maria do Socorro Rocha; Canuto, Kirley Marques; Martins, Camila Mota; Furtado, Roselayne Ferro] Embrapa Agroind Trop, BR-60511110 Fortaleza, Ceara, Brazil. [Laurentino, Larissa da Silva; Frota Silva, Sarah Maria] Univ Fed Ceara, Fortaleza, Ceara, Brazil. [Mendes, Luana Guabiraba] Univ Estadual Ceara, Fortaleza, Ceara, Brazil. [Kim, Sanghoon; Biswas, Atanu] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Cheng, H. N.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Bastos, MDR (reprint author), Embrapa Agroind Trop, Dra Sara Mesquita 2270, BR-60511110 Fortaleza, Ceara, Brazil. EM socorro.bastos@embrapa.br FU Embrapa Agroindustria Tropical; CNPq [405506/2013-9] FX Acknowledgement is made to Gary Kuziar for preparing the samples and gathering most of the data in Table 2. The authors are thankful to Embrapa Agroindustria Tropical for their support. Atanu Biswas and Roselayne Ferro Furtado thank CNPq (process no. 405506/2013-9) for the support of this work through the Science without Borders program. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 1 Z9 1 U1 5 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0142-9418 EI 1873-2348 J9 POLYM TEST JI Polym. Test PD FEB PY 2016 VL 49 BP 156 EP 161 DI 10.1016/j.polymertesting.2015.11.006 PG 6 WC Materials Science, Characterization & Testing; Polymer Science SC Materials Science; Polymer Science GA DB0UE UT WOS:000368222200022 ER PT J AU Weber, MN Mosena, ACS Simoes, SVD Almeida, LL Pessoa, CRM Budaszewski, RF Silva, TR Ridpath, JF Riet-Correa, F Driemeier, D Canal, CW AF Weber, M. N. Mosena, A. C. S. Simoes, S. V. D. Almeida, L. L. Pessoa, C. R. M. Budaszewski, R. F. Silva, T. R. Ridpath, J. F. Riet-Correa, F. Driemeier, D. Canal, C. W. TI Clinical Presentation Resembling Mucosal Disease Associated with 'HoBi'-like Pestivirus in a Field Outbreak SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE pestivirus; diagnosis; mucosal disease; 'HoBi'-like pestivirus; cattle; pathology ID VIRAL DIARRHEA VIRUS; ATYPICAL BOVINE PESTIVIRUS; GRANDE-DO-SUL; PERSISTENT INFECTION; ANTIGENIC CHARACTERIZATION; CALVES; CATTLE; CLASSIFICATION; PIGS; IDENTIFICATION AB The genus Pestivirus of the family Flaviviridae consists of four recognized species: Bovine viral diarrhoea virus 1 (BVDV-1), Bovine viral diarrhoea virus 2 (BVDV-2), Classical swine fever virus (CSFV) and Border disease virus (BDV). Recently, atypical pestiviruses ('HoBi'-like pestiviruses) were identified in batches of contaminated foetal calf serum and in naturally infected cattle with and without clinical symptoms. Here, we describe the first report of a mucosal disease-like clinical presentation (MD) associated with a 'HoBi'-like pestivirus occurring in a cattle herd. The outbreak was investigated using immunohistochemistry, antibody detection, viral isolation and RT-PCR. The sequence and phylogenetic analysis of 5'NCR, N-pro and E2 regions of the RT-PCR positive samples showed that four different 'HoBi'-like strains were circulating in the herd. The main clinical signs and lesions were observed in the respiratory and digestive systems, but skin lesions and corneal opacity were also observed. MD characteristic lesions and a pestivirus with cytopathic biotype were detected in one calf. The present study is the first report of a MD like presentation associated with natural infection with 'HoBi'-like pestivirus. This report describes the clinical signs and provides a pathologic framework of an outbreak associated with at least two different 'HoBi'-like strains. Based on these observations, it appears that these atypical pestiviruses are most likely underdiagnosed in Brazilian cattle. C1 [Weber, M. N.; Mosena, A. C. S.; Budaszewski, R. F.; Canal, C. W.] Univ Fed Rio Grande do Sul, Fac Vet, Lab Virol, BR-91540000 Porto Alegre, RS, Brazil. [Simoes, S. V. D.; Pessoa, C. R. M.; Silva, T. R.; Riet-Correa, F.] Univ Fed Campina Grande, Ctr Saude & Tecnol Rural, Hosp Vet, Patos de Minas, Paraiba, Brazil. [Almeida, L. L.] Inst Pesquisas Vet Desiderio Finamor, FEPAGRO Saude Anim, Eldorado Do Sul, RS, Brazil. [Ridpath, J. F.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Driemeier, D.] Univ Fed Rio Grande do Sul, Fac Vet, Setor Patol Vet, BR-91540000 Porto Alegre, RS, Brazil. RP Weber, MN (reprint author), Univ Fed Rio Grande do Sul, Fac Vet, Lab Virol, BR-91540000 Porto Alegre, RS, Brazil. EM matheusnweber@gmail.com RI Driemeier, David/H-3461-2012; OI Driemeier, David/0000-0003-3766-0654; Canal, Claudio/0000-0002-0621-243X; Weber, Matheus/0000-0001-8282-6778 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES); Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ); Propesq/UFRGS FX Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Apoio a Pesquisa do Estado da Paraiba (FAPESQ) and Propesq/UFRGS supported this work. NR 45 TC 6 Z9 7 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD FEB PY 2016 VL 63 IS 1 BP 92 EP 100 DI 10.1111/tbed.12223 PG 9 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DA8YV UT WOS:000368093800023 PM 24735072 ER PT J AU Ludi, A Ahmed, Z Pomeroy, LW Pauszek, SJ Smoliga, GR Moritz, M Dickmu, S Abdoulkadiri, S Arzt, J Garabed, R Rodriguez, LL AF Ludi, A. Ahmed, Z. Pomeroy, L. W. Pauszek, S. J. Smoliga, G. R. Moritz, M. Dickmu, S. Abdoulkadiri, S. Arzt, J. Garabed, R. Rodriguez, L. L. TI Serotype Diversity of Foot-and-Mouth-Disease Virus in Livestock without History of Vaccination in the Far North Region of Cameroon SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE foot-and-mouth disease virus; Cameroon; Africa; phylogeny; serotyping; SAT2 ID KIDNEY-CELL LINE; MOLECULAR EPIDEMIOLOGY; ADAMAWA PROVINCE; CATTLE; SITE AB Little information is available about the natural cycle of foot-and-mouth disease (FMD) in the absence of control measures such as vaccination. Cameroon presents a unique opportunity for epidemiological studies because FMD vaccination is not practiced. We carried out a prospective study including serological, antigenic and genetic aspects of FMD virus (FMDV) infections among different livestock production systems in the Far North of Cameroon to gain insight into the natural ecology of the virus. We found serological evidence of FMDV infection in over 75% of the animals sampled with no significant differences of prevalence observed among the sampled groups (i.e. market, sedentary, transboundary trade and mobile). We also found antibodies reactive to five of the seven FMDV serotypes (A, O, SAT1, SAT2 and SAT3) among the animals sampled. Finally, we were able to genetically characterize viruses obtained from clinical and subclinical FMD infections in Cameroon. Serotype O viruses grouped into two topotypes (West and East Africa). SAT2 viruses grouped with viruses from Central and Northern Africa, notably within the sublineage causing the large epidemic in Northern Africa in 2012, suggesting a common origin for these viruses. This research will guide future interventions for the control of FMD such as improved diagnostics, guidance for vaccine formulation and epidemiological understanding in support of the progressive control of FMD in Cameroon. C1 [Ludi, A.; Ahmed, Z.; Pauszek, S. J.; Smoliga, G. R.; Arzt, J.; Rodriguez, L. L.] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Ludi, A.; Ahmed, Z.] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr, Res Participat Program, Oak Ridge, TN USA. [Pomeroy, L. W.; Garabed, R.] Ohio State Univ, Dept Vet Prevent Med, Columbus, OH 43210 USA. [Moritz, M.] Ohio State Univ, Dept Anthropol, Columbus, OH 43210 USA. [Dickmu, S.; Abdoulkadiri, S.] Natl Vet Lab, Lanavet, Garoua, Cameroon. [Garabed, R.] Ohio State Univ, Publ Hlth Preparedness Infect Dis Program, Columbus, OH 43210 USA. RP Rodriguez, LL (reprint author), ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM luis.rodriguez@ars.usda.gov OI Arzt, Jonathan/0000-0002-7517-7893 FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-12-X-00060]; National Science Foundation [DEB-1015908]; Eunice Kennedy Shriver National Institute of Child Health & Human Development [R24-HD058484]; Public Health Preparedness for Infectious Diseases program at the Ohio State University; Plum Island Animal Disease Center Research Participation Program fellowship FX The authors would like to thank Mike Larocco for his technical support, Penny Rempe for administrative assistance, Tim Vojt for help with Table 1 and Figure 3 and Karla Moreno Torres for helpful discussions. This project was funded through an inter-agency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-12-X-00060, award number DEB-1015908 from the National Science Foundation, award number R24-HD058484 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development awarded to the Ohio State University Initiative in Population Research and a pilot grant awarded by the Public Health Preparedness for Infectious Diseases program at the Ohio State University. Zaheer Ahmed and Anna Ludi are the recipients of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of the USDA, DOE, or ORAU/ORISE. LANAVET, The Ohio State University and USDA-ARS FADRU are members of the Global Foot-and-Mouth Disease Research Alliance (GFRA). NR 34 TC 2 Z9 2 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD FEB PY 2016 VL 63 IS 1 BP E27 EP E38 DI 10.1111/tbed.12227 PG 12 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DA8YV UT WOS:000368093800003 PM 24735162 ER PT J AU Schalk, CM Saenz, D AF Schalk, Christopher M. Saenz, Daniel TI Environmental drivers of anuran calling phenology in a seasonal Neotropical ecosystem SO AUSTRAL ECOLOGY LA English DT Article DE amphibian; Gran Chaco; photoperiod; reproductive mode; temporal community structure; weather ID REPRODUCTIVE CHARACTER DISPLACEMENT; RAIN-FOREST FROGS; CLIMATE-CHANGE; CHIQUITANO REGION; ACTIVITY PATTERNS; COMMUNITIES; ASSEMBLAGE; DIVERSITY; PREDATION; BEHAVIOR AB Temporal variation represents an important component in understanding the structure of ecological communities and species coexistence. We examined calling phenology of an assemblage of anurans in the Gran Chaco ecoregion of Bolivia by deploying automated recording devices to document nocturnally vocalizing amphibians nightly at seven ponds from 20 January 2011 until 31 October 2011. Using logistic regression, we modelled the relationships between temperature, rainfall and photoperiod with calling activity. There was a distinct seasonal effect with calling activity concentrated in the rainy season with no species detected during the dry season from June until the end of October. Calling activity was positively and significantly correlated with photoperiod in 9 of the 10 species analyzed, but there were distinct species-specific relationships associated with rainfall and temperature. All of these species utilize ephemeral ponds as breeding sites, which can account for their reliance on rainfall as an important driver in calling activity. Two prolonged breeders exhibited similar seasonal breeding patterns across the rainy season, but differed in their response to daily abiotic factors, which might be attributed to the constraints imposed by their reproductive mode. Explosive breeders needed several days of rain to elicit calling. Two pairs of congeners had distinct species-specific relationships between their calling activity and abiotic factors, even though the congeners shared the same reproductive mode, suggesting that the reproductive modes vary in the constraints imposed on calling activity. The patterns observed suggest that calling phenology of tropical anurans is determined by the interaction of exogenous factors (i.e. climatic variables) and endogenous factors (i.e. reproductive modes). C1 [Schalk, Christopher M.] Texas A&M Univ, Dept Wildlife & Fisheries Sci, Ecol & Evolutionary Biol Program, College Stn, TX 77843 USA. [Schalk, Christopher M.] Texas A&M Univ, Biodivers Res & Teaching Collect, College Stn, TX 77843 USA. [Saenz, Daniel] US Forest Serv, Southern Res Stn, Nacogdoches, TX USA. RP Schalk, CM (reprint author), Texas A&M Univ, Dept Wildlife & Fisheries Sci, Ecol & Evolutionary Biol Program, 210 Nagle Hall, College Stn, TX 77843 USA. EM cschalk@tamu.edu FU National Science Foundation's Graduate Research Fellowship Program; Applied Biodiversity Science NSF-IGERT Program at Texas A&M University (NSF-IGERT Award) [0654377]; Southern Research Station, U.S. Forest Service FX We thank the Capitania del Alto y Bajo Isoso (CABI) for permission to conduct research in Isoso, and R.L. Cuellar for providing logistical support while in Bolivia. K. Rivero at the Museo Noel Kempff Mercado assisted with permit support. We thank J. Childress and N. Aall for their assistance with transcribing frog calls, N. Koerth for assistance with statistical analyses, and C.G. Montana and two anonymous reviewers for constructive comments on the manuscript. Support was provided by the National Science Foundation's Graduate Research Fellowship Program, the Applied Biodiversity Science NSF-IGERT Program at Texas A&M University (NSF-IGERT Award # 0654377), and the Southern Research Station, U.S. Forest Service. This is publication number 1503 of the Biodiversity Research and Teaching Collections at Texas A&M University. NR 54 TC 4 Z9 4 U1 5 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1442-9985 EI 1442-9993 J9 AUSTRAL ECOL JI Austral Ecol. PD FEB PY 2016 VL 41 IS 1 BP 16 EP 27 DI 10.1111/aec.12281 PG 12 WC Ecology SC Environmental Sciences & Ecology GA DA3US UT WOS:000367725200003 ER PT J AU De Pra, MC Kunz, A Bortoli, M Scussiato, LA Coldebella, A Vanotti, M Soares, HM AF De Pra, Marina C. Kunz, Airton Bortoli, Marcelo Scussiato, Lucas A. Coldebella, Arlei Vanotti, Matias Soares, Hugo M. TI Kinetic models for nitrogen inhibition in ANAMMOX and nitrification process on deammonification system at room temperature SO BIORESOURCE TECHNOLOGY LA English DT Article DE ANAMMOX; Deammonification; Kinetic models; Partial nitritation; Specific nitrogen removal rate ID WASTE-WATER; SUBSTRATE-INHIBITION; AMBIENT-TEMPERATURE; REMOVAL; AMMONIUM; NITRITE; CULTURE; OXIDATION; IDENTIFICATION; PERFORMANCE AB In this study were fitted the best kinetic model for nitrogen removal inhibition by ammonium and/or nitrite in three different nitrogen removal systems operated at 25 degrees C: a nitrifying system (NF) containing only ammonia oxidizing bacteria (AOB), an ANAMMOX system (AMX) containing only ANAMMOX bacteria, and a deammonification system (DMX) containing both AOB and ANAMMOX bacteria. NF system showed inhibition by ammonium and was best described by Andrews model. The AMX system showed a strong inhibition by nitrite and Edwards model presented a best system representation. For DMX system, the increased substrate concentration (until 1060 mg NH3-N/L) tested was not limiting for the ammonia consumption rate and the Monod model was the best model to describe this process. The AOB and ANAMMOX sludges combined in the DMX system displayed a better activity, substrate affinity and excellent substrate tolerance than in nitrifying and ANAMMOX process. (C) 2015 Elsevier Ltd. All rights reserved. C1 [De Pra, Marina C.; Soares, Hugo M.] Univ Fed Santa Catarina, Dept Chem Engn, Florianopolis, SC, Brazil. [Kunz, Airton; Coldebella, Arlei] Embrapa Swine & Poultry, Concordia, SC, Brazil. [Kunz, Airton; Scussiato, Lucas A.] PGEAGRI CCET UNIOESTE, Dept Agr Engn, Cascavel, PR, Brazil. [Bortoli, Marcelo] Fed Technol Univ Parana, Dept Environm Engn, Francisco Beltrao, PR, Brazil. [Vanotti, Matias] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP De Pra, MC (reprint author), BR-153,Km 110, BR-89700000 Concordia, SC, Brazil. EM marinacdepra@gmail.com FU CAPES; CNPq FX Authors thanks financial support from CAPES and CNPq. NR 35 TC 0 Z9 0 U1 10 U2 55 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2016 VL 202 BP 33 EP 41 DI 10.1016/j.biortech.2015.11.048 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DA3CM UT WOS:000367673500005 PM 26700756 ER PT J AU Van Weelden, MB Andersen, DS Kerr, BJ Trabue, SL Pepple, LM AF Van Weelden, M. B. Andersen, D. S. Kerr, B. J. Trabue, S. L. Pepple, L. M. TI Impact of fiber source and feed particle size on swine manure properties related to spontaneous foam formation during anaerobic decomposition SO BIORESOURCE TECHNOLOGY LA English DT Article DE Manure; Foaming; Anaerobic digestion; Feedstock composition; Microbial community ID BIOGAS REACTORS; EMISSIONS; DIGESTION AB Foam accumulation in deep-pit manure storage facilities is of concern for swine producers because of the logistical and safety-related problems it creates. A feeding trial was performed to evaluate the impact of feed grind size, fiber source, and manure inoculation on foaming characteristics. Animals were fed: (1) C-SBM (corn-soybean meal): (2) C-DDGS (corn-dried distiller grains with solubles); and (3) C-Soybean Hull (corn-soybean meal with soybean hulls) with each diet ground to either fine (374 mu m) or coarse (631 mu m) particle size. Two sets of 24 pigs were fed and their manure collected. Factors that decreased feed digestibility (larger grind size and increased fiber content) resulted in increased solids loading to the manure, greater foaming characteristics, more particles in the critical particle size range (2-25 mu m), and a greater biological activity/potential. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Van Weelden, M. B.; Andersen, D. S.] Iowa State Univ, Agr & Biosyst Engn, Ames, IA 50011 USA. [Kerr, B. J.; Trabue, S. L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Pepple, L. M.] Univ Illinois, Agr & Biol Engn, Urbana, IL 61801 USA. RP Andersen, DS (reprint author), Iowa State Univ, Agr & Biosyst Engn, Ames, IA 50011 USA. EM dsa@iastate.edu FU Iowa Pork Producers Association FX Funding for this work was provided by the Iowa Pork Producers Association. NR 28 TC 2 Z9 2 U1 5 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2016 VL 202 BP 84 EP 92 DI 10.1016/j.biortech.2015.11.080 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DA3CM UT WOS:000367673500011 PM 26702515 ER PT J AU Thomas, RE Buehlmann, U AF Thomas, R. Edward Buehlmann, Urs TI Potential for Yield Improvement in Combined Rip-First and Crosscut-First Rough Mill Processing SO BIORESOURCES LA English DT Article DE Rough mill lumber yield; Cut-up systems; Rip-first; Crosscut-first; Performance ID CUTTING-BILL REQUIREMENTS; CHARACTER-MARKED FURNITURE; LUMBER YIELD; DESIGN; PERFORMANCE; INCREASE; PARTS AB Traditionally, lumber cutting systems in rough mills have either first ripped lumber into wide strips and then crosscut the resulting strips into component lengths (rip-first), or first crosscut the lumber into component lengths, then ripped the segments to the required widths (crosscut-first). Each method has its advantages and disadvantages. Crosscut-first typically works best for the production of wider components, while rip-first favors the production of narrower and longer components. Thus, whichever type of processing method is selected for a given rough mill usually depends on the characteristics of the cutting bills the mill expects to process. There is a third option, a dual-line mill that contains both ripfirst and crosscut-first processing streams. To date, such mills have been rare for a variety of reasons, complexity and cost being among them. However, dual-line systems allow the mill to respond to varying cutting bill size demands as well as to board characteristics that favor one method (rip-first or crosscut-first) over the other. Using the Rough Mill Simulator (ROMI 4), this paper examines the yield improvement potential of dual-line processing over single-system processing (i.e., rip-first or crosscut-first processing alone) for a variety of cutting bills and lumber grade mixes. C1 [Thomas, R. Edward] US Forest Serv, USDA, No Res Stn, Princeton, WV 24740 USA. [Buehlmann, Urs] Virginia Tech, Dept Sustainable Biomat, Blacksburg, VA USA. RP Thomas, RE (reprint author), US Forest Serv, USDA, No Res Stn, Princeton, WV 24740 USA. EM ethomas@fs.fed.us FU USDA Forest Service, Northern Research Station, Princeton, WV FX The work upon which this publication is based was funded in part through the USDA Forest Service, Northern Research Station, Princeton, WV. NR 40 TC 0 Z9 0 U1 0 U2 0 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD FEB PY 2016 VL 11 IS 1 BP 1477 EP 1493 PG 17 WC Materials Science, Paper & Wood SC Materials Science GA DA3WX UT WOS:000367732700116 ER PT J AU Polyakov, VO Nearing, MA Stone, JJ Collins, CDH Nichols, MH AF Polyakov, V. O. Nearing, M. A. Stone, J. J. Collins, C. D. Holifield Nichols, M. H. TI Quantifying decadal-scale erosion rates and their short-term variability on ecological sites in a semi-arid environment SO CATENA LA English DT Article DE Soil erosion; Cesium; Semiarid; Runoff; State and Transition Model ID SOIL AGGREGATE STABILITY; SEDIMENT YIELD; CESIUM-137 MEASUREMENTS; RANGELAND HEALTH; WESTERN JUNIPER; RUNOFF; TRANSITION; CLIMATE; REDISTRIBUTION; ENCROACHMENT AB Soil erosion rates on six semi-arid loamy upland rangeland sites located in southeastern Arizona were measured using a rainfall simulator and Cs-137 fallout methods. Site characteristics that have the greatest effects on soil. erosion and runoff were identified. Long term (50 years) soil erosion rates as estimated using Cs-137 method varied between 5.1 and 11.0 Mg ha(-1) y(-1) and showed significant differences between Historic Climax Plant Community and Mesquite/Native states within the State and Transition Model. Erosion rates under simulated rainfall were measured between 0.9 and 17.2 g m(-2) min(-1) at 175 mm h(-1) precipitation across all sites and varied as much as 8-fold at the same location, depending on the time of the simulation. Temporal variability of erosion rates within a site was in some cases much greater than inter-site differences. This variability was attributed to natural or management driven changes in plant community and soil characteristics. Bare soil area, an aggregate indicator for all types of cover combined, was the main controlling factor of erosion process across ecological sites. For meaningful interpretation rainfall simulation, results must be placed in the context of the range of possible vegetation and surface conditions within a given ecological site. (C) 2015 Elsevier B.V. All rights reserved. C1 [Polyakov, V. O.; Nearing, M. A.; Stone, J. J.; Collins, C. D. Holifield; Nichols, M. H.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85718 USA. RP Polyakov, VO (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allan Rd, Tucson, AZ 85718 USA. EM viktor.polyakov@ars.usda.gov NR 50 TC 0 Z9 0 U1 1 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD FEB PY 2016 VL 137 BP 501 EP 507 DI 10.1016/j.catena.2015.10.023 PG 7 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DA2ON UT WOS:000367635800048 ER PT J AU Xu, YL Woeste, K Cai, NH Kang, XY Li, GQ Chen, S Duan, A AF Xu, Yulan Woeste, Keith Cai, Nianhui Kang, Xiangyang Li, Genqian Chen, Shi Duan, Anan TI Variation in needle and cone traits in natural populations of Pinus yunnanensis SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Genetic diversity; Conservation; Yunnan pine; Cone morphology; Needle morphology ID GENETIC-VARIATION; SCOTS PINE; MORPHOLOGICAL CHARACTERS; PROVENANCE VARIATION; SEED CHARACTERS; SYLVESTRIS L.; BLUE PINE; TURKEY; CANARIENSIS; WALLICHIANA AB Pinus yunnanensis Franch. is a major component of coniferous forests in southwestern China. Little is known about its intraspecific variation. Morphological variations in needle and cone traits of P. yunnanensis were analyzed to quantify variability among and within natural populations. Seven traits were measured on 10 needles collected from 30 trees in each of the 18 sampled populations of P. yunnanensis. Four cone traits were measured in 221 individual trees from nine populations. The results showed that there were significant differences (p < 0.01) both among populations and within populations in each needle and cone trait. The proportion of phenotypic variation of nearly all needle and cone traits was over 50 % within populations, which showed trees within populations accounted for a majority of the total variation. The needle traits showed higher variability within population than cone traits. Variability in the needle traits was correlated with geo-climatic parameters (longitude, latitude, altitude, temperature, and precipitation). Needle length and the ratio of needle length to fascicle sheath length showed clinal variation in response to latitudinal and altitudinal gradients. A hierarchical classification of all populations based on needle traits led to the formation of four major groups. The findings provide important genetic information for the evaluation of variation. Moreover, it will assist in management of genetic diversity of P. yunnanensis. C1 [Xu, Yulan; Cai, Nianhui; Li, Genqian; Chen, Shi; Duan, Anan] Southwest Forestry Univ, Key Lab Forest Resources Conservat & Use Southwes, Coll Forestry, Kunming 650224, Peoples R China. [Xu, Yulan; Kang, Xiangyang] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, State Engn Lab Tree Breeding, Beijing 100083, Peoples R China. [Woeste, Keith] Purdue Univ, US Forest Serv, USDA, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47906 USA. RP Woeste, K (reprint author), Purdue Univ, US Forest Serv, USDA, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47906 USA. EM woeste@purdue.edu FU National Natural Science Foundation of China [NSFC 31260191]; Natural Science Foundation of Yunnan Province, China [2010CD065] FX This study was financially supported by the National Natural Science Foundation of China (NSFC 31260191), Natural Science Foundation of Yunnan Province, China (2010CD065). NR 45 TC 2 Z9 3 U1 2 U2 13 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD FEB PY 2016 VL 27 IS 1 BP 41 EP 49 DI 10.1007/s11676-015-0153-6 PG 9 WC Forestry SC Forestry GA DA0XG UT WOS:000367520300005 ER PT J AU Visscher, RS Nassauer, JI Marshall, LL AF Visscher, Rachel Stehouwer Nassauer, Joan Iverson Marshall, Lauren Lesch TI Homeowner preferences for wooded front yards and backyards: Implications for carbon storage SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Climate change; Pen-urban; Residential landscape; Suburban; Urban forest ID RESIDENTIAL LANDSCAPES; HOUSEHOLD BEHAVIORS; PARCEL SIZE; URBAN; MANAGEMENT; TREES; SEQUESTRATION; NEIGHBORHOODS; PERCEPTIONS; ATTITUDES AB This study contributes to understanding of social preferences, norms, and behaviors in residential landscapes that affect planning, design, and management of trees, which store carbon and contribute to mitigating climate change. We investigated southeast Michigan homeowner preferences for different styles of front yards and backyards that were more or less wooded, learning what landscape characteristics they preferred and how their preferences related to their own yard management behavior. We surveyed homeowners, who selected their most preferred front yard and backyard from a series of images and indicated what characteristics were important to their preferences. We developed a homeowner typology based on their stated preferences for more and less wooded front yards and backyards, distinguished each type by landscape characteristics that were most important to homeowners, and tested whether homeowners of each preference type managed their actual yards consistent with type. Our results show that homeowners are heterogeneous in their preferences, identifying different characteristics as important according to type, but that only mowing of their actual yard is consistent with type. We also found that both important characteristics and actual uses of homeowners' yards varied between front yards and backyards. Both homeowner types and front yard/backyard differences suggest opportunities for planning and maintaining larger urban woodlands on residential lots. (C) 2015 Elsevier B.V. All rights reserved. C1 [Visscher, Rachel Stehouwer; Nassauer, Joan Iverson] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. [Marshall, Lauren Lesch] US Forest Serv, Washington, DC USA. RP Visscher, RS (reprint author), HT Harvey & Associates 983 Univ Ave,Bldg D, Los Gatos, CA 95032 USA. EM rvisscher@harveyecology.com; nassauer@umich.edu; lemarshall@fs.fed.us FU Environmental Protection Agency STAR Fellowship [FP91750901-1]; National Science Foundation's program on the Dynamics of Coupled Natural and Human Systems [GEO-0814542] FX We gratefully acknowledge the support of the Environmental Protection Agency STAR Fellowship (Assistance Agreement FP91750901-1) and the support from the National Science Foundation's program on the Dynamics of Coupled Natural and Human Systems (grant #GEO-0814542) for Project SLUCE II. NR 48 TC 1 Z9 1 U1 13 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD FEB PY 2016 VL 146 BP 1 EP 10 DI 10.1016/j.landurbplan.2015.09.001 PG 10 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA DA4KF UT WOS:000367768100001 ER PT J AU Kunwar, B Cheng, HN Chandrashekaran, SR Sharma, BK AF Kunwar, Bidhya Cheng, H. N. Chandrashekaran, Sriram R. Sharma, Brajendra K. TI Plastics to fuel: a review SO RENEWABLE & SUSTAINABLE ENERGY REVIEWS LA English DT Review DE Plastic waste; Plastics pyrolysis; Catalytic conversion; Gasoline; Diesel; Co-pyrolysis ID HIGH-DENSITY POLYETHYLENE; OIL-BASED LUBRICANTS; REFUSE-DERIVED FUEL; WASTE PLASTICS; CATALYTIC DEGRADATION; THERMAL-DEGRADATION; PYROLYSIS KINETICS; VEGETABLE-OIL; SOLID-WASTES; LIQUEFACTION AB The thermal and catalytic processes of converting waste plactics into fuels are promising techniques to eliminate the refuse which otherwise is harmful to the enivironment, and decrease the dependence on fossil fuels. Thermal degradation decomposes plastic into three fractions: gas, crude oil, and solid residue. Crude oil from non-catalytic pyrolysis is usually composed of higher boiling point hydrocarbons. The optimization of conversion parameters such as the choice of catalyst, reactor design, pyrolysis temperature, and plastic-to-catalyst ratio plays a very important role in the efficient generation of gasoline and diesel grade fuel. The use of a catalyst for thermal conversion lowers the energy required for conversion, and the catalyst choice is important for efficient fuel production. The suitable selection of catalysts can increase the yield of crude oil with lower hydrocarbon content. Co-pyrolysis of plastics with coal or shale oil improves crude oil quality by decreasing its viscosity. A large number of publications have appeared on various processes, and continued improvements and/or innovations are expected in the future. Further investigations on the catalytic systems are required in order to advance the field, particularly to enhance the added value of fuels and to minimize the use of energy. This review aims to provide both the highlights of the remarkable achievements of this field and the milestones that need to be achieved in the future. (c) 2015 Elsevier Ltd. All rights reserved. C1 [Kunwar, Bidhya; Chandrashekaran, Sriram R.; Sharma, Brajendra K.] Univ Illinois, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. [Cheng, H. N.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Kunwar, B (reprint author), Univ Illinois, Illinois Sustainable Technol Ctr, 1 Hazelwood Dr, Champaign, IL 61820 USA. EM bkunwar@illinois.edu; hn.cheng@ars.usda.gov; schandr@illinois.edu; bksharma@illinois.edu NR 80 TC 10 Z9 10 U1 29 U2 76 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1364-0321 J9 RENEW SUST ENERG REV JI Renew. Sust. Energ. Rev. PD FEB PY 2016 VL 54 BP 421 EP 428 DI 10.1016/j.rser.2015.10.015 PG 8 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA DA4GM UT WOS:000367758200034 ER PT J AU Shappell, NW Billey, LO Shipitalo, MJ AF Shappell, N. W. Billey, L. O. Shipitalo, M. J. TI Estrogenic activity and nutrient losses in surface runoff after winter manure application to small watersheds SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE E-Screen; Swine manure; Turkey litter; Frozen fields ID STEROID ESTROGENS; POULTRY LITTER; BUFFER STRIPS; 17-BETA-ESTRADIOL; SOIL; TESTOSTERONE; EFFLUENTS; DAIRY AB Confined Animal Feeding Operations generate large amounts of wastes that are land-applied to provide nutrients for crop production and return organic matter to the soil. Production practices and storage limitations often necessitate that wastes be applied to frozen and snow-covered soil. Use of application setbacks have reduced concerns related to nutrient losses in surface runoff from manure, but the estrogenic activity of runoff under these conditions has not been evaluated. Therefore, we measured and sampled surface runoff when manure was applied in the winter at a rate to meet crop N needs and measured estradiol equivalents (E(2)Eqs) using E-Screen. In year one, six small watersheds used to produce corn were evaluated, treatments: 2 no-manure controls, 2 liquid swine manure with 30-m setbacks, and 2 turkey litter with 30-m setbacks. In addition, beef manure was applied to six frozen plots of forage. For years 2 and 3, applications were repeated on the swine manure watersheds and one control watershed. E(2)Eqs and nutrient concentrations generally peaked in the first runoff event after application. The highest measured E(2)Eq (5.6 ng L-1) was in the first event after swine manure application and was less than the 8.9 ng L-1 Lowest Observable Effect Concentration (LOEC) for aquatic species and well below the concentrations measured in other studies using ELISAs to measure hormone concentrations. No runoff occurred from plots planted with forage, indicating low risk for environmental impact, and therefore plots were discontinued from study. In years 2 and 3, estrogenic activity never exceeded the Predicted No Effect Concentrations for E-2 of 2 ng L-1. When post-application runoff contained high estrogenic activity, strong correlations (R-2 0.86 to 0.96) of E(2)Eq to Ca2+, Mg2+, and K+ concentrations were observed, indicating under some condition these cations might be useful surrogates for E(2)Eq measurements. Published by Elsevier B.V. C1 [Shappell, N. W.; Billey, L. O.] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. [Shipitalo, M. J.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Shappell, NW (reprint author), ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM nancy.shappell@ars.usda.gov; Lloyd.Billey@ars.usda.gov; Martin.Shipitalo@ars.usda.gov OI Shipitalo, Martin/0000-0003-4775-7345 NR 31 TC 2 Z9 2 U1 6 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 1 PY 2016 VL 543 BP 570 EP 580 DI 10.1016/j.scitotenv.2015.10.130 PN A PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DA2OL UT WOS:000367635600057 PM 26610286 ER PT J AU Liu, SS Zhu, YR Meng, W He, ZQ Feng, WY Zhang, C Giesy, JP AF Liu, Shasha Zhu, Yuanrong Meng, Wei He, Zhongqi Feng, Weiying Zhang, Chen Giesy, John P. TI Characteristics and degradation of carbon and phosphorus from aquatic macrophytes in lakes: Insights from solid-state C-13 NMR and solution P-31 NMR spectroscopy SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Plant-derived water extractable organic matter; Nuclear magnetic resonance spectroscopy; Phosphorus; Sediment; Eutrophication ID DISSOLVED ORGANIC-MATTER; SOIL; DECOMPOSITION; SEDIMENTS; WATER; TRANSFORMATIONS; NITROGEN; SORPTION; BIOAVAILABILITY; STABILIZATION AB Water extractable organic matter (WEOM) derived from macrophytes plays an important role in biogeo-chemical cycling of nutrients, including carbon (C), nitrogen (N) and phosphorus (P) in lakes. However, reports of their composition and degradation in natural waters are scarce. Therefore, compositions and degradation of WEOM derived from six aquatic macrophytes species of Tai Lake, China, were investigated by use of solid-state C-13 NMR and solution P-31 NMR spectroscopy. Carbohydrates were the predominant constituents of WEOM fractions, followed by carboxylic acid. Orthophosphate (ortho-P) was the dominant form of P (78.7% of total dissolved P) in the water extracts, followed by monoester P (mono-P) (20.6%) and little diester P (0.65%). The proportion of mono-Pin total P species increased with the percentage of O-alkyl and O-C-O increasing in the WEOM, which is likely due to degradation and dissolution of biological membranes and RNA from aquatic plants. Whereas the proportion of mono-P decreased with alkyl-C, NCH/OCH3 and COO/N-C=O increasing, which may be owing to the insoluble compounds including C functional groups of alkyl-C, NCH/OCH3 and COO/N-C=O, such as aliphatic biopolymers, lignin and peptides. Based on the results of this study and information in the literature about water column and sediment, we propose that WEOM, dominated by polysaccharides, are the most labile and bioavailable component in debris of macrophytes. Additionally, these WEOMs would also be a potential source for bioavailable organic P (e.g., RNA, DNA and phytate) for lakes. (C) 2015 Elsevier B.V. All rights reserved. C1 [Liu, Shasha; Feng, Weiying] Beijing Normal Univ, Coll Water Sci, Beijing 100875, Peoples R China. [Liu, Shasha; Zhu, Yuanrong; Meng, Wei; Feng, Weiying; Zhang, Chen; Giesy, John P.] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. [He, Zhongqi] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Giesy, John P.] Univ Saskatchewan, Dept Biomed & Vet Biosci, Saskatoon, SK, Canada. [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK, Canada. RP Zhu, YR (reprint author), Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. EM zhuyuanrong07@mails.ucas.ac.cn; mengwei@craes.org.cn FU National Natural Science Foundation of China [41403094, 41130743, 41261140337] FX This research was jointly supported by National Natural Science Foundation of China (No. 41403094, 41130743 and 41261140337). NR 62 TC 4 Z9 4 U1 14 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 1 PY 2016 VL 543 BP 746 EP 756 DI 10.1016/j.scitotenv.2015.11.080 PN A PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DA2OL UT WOS:000367635600075 PM 26624522 ER PT J AU Olsoy, PJ Mitchell, JJ Levia, DF Clark, PE Glenn, NF AF Olsoy, Peter J. Mitchell, Jessica J. Levia, Delphis F. Clark, Patrick E. Glenn, Nancy F. TI Estimation of big sagebrush leaf area index with terrestrial laser scanning SO ECOLOGICAL INDICATORS LA English DT Article DE Artemisia tridentata; Convex hull volume; Ground-based LiDAR; Leaf area index; Terrestrial laser scanning; Voxel volume ID MODELING EVAPOTRANSPIRATION; ABOVEGROUND BIOMASS; VEGETATION INDEXES; STEPPE ECOSYSTEM; AIRBORNE LIDAR; WOODY-PLANTS; VOXEL SIZE; COMMUNITIES; DENSITY; REFLECTANCE AB Accurate monitoring and quantification of the structure and function of semiarid ecosystems is necessary to improve carbon and water flux models that help describe how these systems will respond in the future. The leaf area index (LAI, m(2) m(-2)) is an important indicator of energy, water, and carbon exchange between vegetation and the atmosphere. Remote sensing techniques are frequently used to estimate LA!, and can provide users with scalable measurements of vegetation structure and function. We tested terrestrial laser scanning (TLS) techniques to estimate LAI using structural variables such as height, canopy cover, and volume for 42 Wyoming big sagebrush (Artemisia tridentata subsp. wyomingensis Beetle & Young) shrubs across three study sites in the Snake River Plain, Idaho, USA. The TLS-derived variables were regressed against sagebrush LAI estimates calculated using specific leaf area measurements, and compared with point-intercept sampling, a field method of estimating LAI. Canopy cover estimated with the TLS data proved to be a good predictor of LAI (r(2) = 0.73). Similarly, a convex hull approach to estimate volume of the shrubs from the TLS data also strongly predicted LAI (r(2) = 0.76), and compared favorably to point-intercept sampling (r(2) =0.78), a field-based method used in rangelands. These results, coupled with the relative ease-of-use of TLS, suggest that TLS is a promising tool for measuring LAI at the shrub-level. Further work should examine the structural measures in other similar shrublands that are relevant for upscaling LAI to the plot-level (i.e., hectare) using data from TLS and/or airborne laser scanning and to regional levels using satellite-based remote sensing. (c) 2015 Elsevier Ltd. All rights reserved. C1 [Olsoy, Peter J.; Glenn, Nancy F.] Boise State Univ, Dept Geosci, Boise Ctr Aerosp Lab, Boise, ID 83725 USA. [Mitchell, Jessica J.] Appalachian State Univ, Dept Geog & Planning, Boone, NC 28608 USA. [Levia, Delphis F.] Univ Delaware, Dept Geog, Newark, DE 19716 USA. [Levia, Delphis F.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Clark, Patrick E.] USDA ARS, Boise, ID 83712 USA. RP Glenn, NF (reprint author), Boise State Univ, Dept Geosci, Boise Ctr Aerosp Lab, 1910 Univ Dr, Boise, ID 83725 USA. EM peterolsoy@gmail.com; mitchelljj@appstate.edu; dlevia@udel.edu; pat.clark@ars.usda.gov; nancyglenn@boisestate.edu FU NSF EAR [1355702]; NOAA OAR Earth Systems Research Laboratory/Physical Sciences Division [NA100AR4680240] FX This study was funded by NSF EAR 1355702 and NOAA OAR Earth Systems Research Laboratory/Physical Sciences Division NA100AR4680240. We thank Mr. Randy Lee at Idaho National Laboratory for his assistance with the TLS. NR 63 TC 2 Z9 2 U1 17 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD FEB PY 2016 VL 61 BP 815 EP 821 DI 10.1016/j.ecolind.2015.10.034 PN 2 PG 7 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CZ9IL UT WOS:000367411200063 ER PT J AU Meng, H Li, AY Costa, LM Castro-Arellano, I Liu, JZ AF Meng, Hao Li, Andrew Y. Costa Junior, Livio M. Castro-Arellano, Ivan Liu, Jingze TI Evaluation of DEET and eight essential oils for repellency against nymphs of the lone star tick, Amblyomma americanum (Acari: Ixodidae) SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Repellent; Essential oil; Lone star tick; Amblyomma americanum ID IXODES-SCAPULARIS ACARI; UNITED-STATES; BORNE DISEASES; CULEX-QUINQUEFASCIATUS; DIPTERA-CULICIDAE; AEDES-AEGYPTI; NEW-YORK; HUMANS; PREVENTION; MOSQUITOS AB DEET and Eight commercially available essential oils (oregano, clove, thyme, vetiver, sandalwood, cinnamon, cedarwood, and peppermint) were evaluated for repellency against host-seeking nymphs of the lone star tick, Amblyomma americanum. Concentration-repellency response was established using the vertical paper bioassay technique for each essential oil and compared with that of N,N-diethyl-3-methyl benzamide (DEET), a standard repellent compound present in many commercial repellent formulations. The effective concentration of DEET that repels 50 % of ticks (EC50) was estimated at 0.02 mg/cm(2), while EC(50)s of the essential oils fall between 0.113 and 0.297 mg/cm(2). Based on EC50 estimates, oregano essential oil was the most effective among all essential oils tested, followed by clove, thyme, vetiver, sandalwood, cinnamon, cedarwood, and peppermint oils. None of the tested essential oils demonstrated a level of tick repellency found with DEET. Results from this study illustrated the challenge in search for more effective natural tick repellents. C1 [Meng, Hao; Li, Andrew Y.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Meng, Hao; Liu, Jingze] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Hebei, Peoples R China. [Costa Junior, Livio M.] Univ Fed Maranhao, Biol & Hlth Sci Ctr, BR-65080805 San Luis, MA, Brazil. [Castro-Arellano, Ivan] Texas State Univ, Dept Biol, San Marcos, TX 78666 USA. RP Li, AY (reprint author), ARS, USDA, Invas Insect Biocontrol & Behav Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Andrew.Li@ars.usda.gov RI Costa-Junior, Livio/H-7530-2012 OI Costa-Junior, Livio/0000-0002-1475-049X FU China Scholarship Council; Science Without Borders Program of CAPES, a Foundation affiliated with the Ministry of Education of Brazil; ARS [8042-32000-008-00] FX The authors wish to thank James McCrary of USDA, ARS, Invasive Insect Biocontrol and Behavior Laboratory (IIBBL) for technical support, and Dr. Erika Machtinger (USDA, ARS, IIBBL) for critical review of the manuscript. H. Meng was supported by a scholarship from China Scholarship Council; LM Costa Junior was supported by a Grant from the Science Without Borders Program of CAPES, a Foundation affiliated with the Ministry of Education of Brazil; and AY Li was supported by funds from an ARS in-house Project (#8042-32000-008-00)-Prevention of Arthropod Bites. NR 47 TC 1 Z9 1 U1 2 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD FEB PY 2016 VL 68 IS 2 BP 241 EP 249 DI 10.1007/s10493-015-9994-0 PG 9 WC Entomology SC Entomology GA CZ8HS UT WOS:000367341400009 PM 26590930 ER PT J AU Liu, PW Chandra, A Que, YX Chen, PH Grisham, MP White, WH Dalley, CD Tew, TL Pan, YB AF Liu, Pingwu Chandra, Amaresh Que, Youxiong Chen, Ping-Hua Grisham, Michael P. White, William H. Dalley, Caleb D. Tew, Thomas L. Pan, Yong-Bao TI Identification of quantitative trait loci controlling sucrose content based on an enriched genetic linkage map of sugarcane (Saccharum spp. hybrids) cultivar 'LCP 85-384' SO EUPHYTICA LA English DT Article DE Genetic linkage map; Molecular marker; Quantitative trait loci (QTL); Sucrose content; Sugarcane ID RUST RESISTANCE GENE; MICROSATELLITE MARKERS; COMPLEX TRAITS; COMMERCIAL CROSS; INTERSPECIFIC CROSS; BRASSICA-NAPUS; AFLP MARKERS; QTL ANALYSIS; SSR MARKERS; YIELD AB Sucrose content is one of the most important traits considered in sugarcane breeding. Since sugarcane cultivars possess > 100 chromosomes (2n = 100-130) and are genetically complex polyploid and aneuploids, identification of quantitative trait loci (QTLs) associated with sucrose content is considered the best option to improve sucrose content through molecular breeding. A preliminary genetic linkage map of Louisiana sugarcane cultivar 'LCP 85-384' from a previous study was enriched using 65 additional polymorphism simple-sequence-repeats (SSR) primer-pairs to identify more co-segregated and homologous groups (CGs and HGs) and QTLs controlling sucrose content. Eighty-four SSR primer-pairs produced 456 markers, of which 441 were polymorphic. Both simplex (993) and duplex (225) amplified fragment length polymorphism (AFLP) and target region amplification polymorphism (TRAP) markers reported previously were also included to construct the LCP 85-384 map. These simplex and duplex markers were assigned to 108 CGs successfully using JoinMap(A (R)). This map had a cumulative genome length of 7406.3 cM that included 675 AFLP (69.8 %), 90 TRAP (9.3 %), and 202 SSR (20.9 %) markers. The 202 SSR markers were assigned to 65 CGs and 8 HGs. Based on this map, 24 putative QTLs affecting sucrose content were identified. Five QTLs were unlinked and the other 19 QTLs were located on nine CGs within four HGs. Of these QTLs, 11 had an effect in both plant-cane (20.33 %) and first-stubble (25.68 %) crops. A higher efficiency of QTLs' identification with AFLP, TRAP and SSR markers in such a genetically complex crop, proposes its wider utility in molecular breeding in sugarcane. C1 [Liu, Pingwu; Chandra, Amaresh; Grisham, Michael P.; White, William H.; Dalley, Caleb D.; Tew, Thomas L.; Pan, Yong-Bao] USDA ARS, Sugarcane Res Lab, MSA, Houma, LA 70360 USA. [Liu, Pingwu] Guangxi Univ, Collaborat Innovat Ctr Sugar Ind Guangxi, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Peoples R China. [Chandra, Amaresh] ICAR Indian Inst Sugarcane Res, Lucknow 226002, Uttar Pradesh, India. [Que, Youxiong; Chen, Ping-Hua] Fujian Agr & Forestry Univ, Minist Agr, Key Lab Sugarcane Biol & Genet Breeding, Fuzhou 350002, Peoples R China. RP Pan, YB (reprint author), USDA ARS, Sugarcane Res Lab, MSA, Houma, LA 70360 USA. EM yongbao.pan@ars.usda.gov FU Department of Biotechnology, Government of India FX AFLP and TRAP marker data were kindly provided by Collins Kimbeng and Suman Andru. This research was partially funded by grower/processor check-off funds administrated by the American Sugar Cane League of the USA., Inc., Thibodaux, Louisiana, USA. Greenhouse and field technical supports were provided by Jennifer Chiasson, Elta Duet, Brian Duet, Cory Landry, Lionel Lomax, Jeri Maggio, Norris Matherne, Eric Petrie, Clinton Randall, Randy Richard, David Verdun, and Kathy Warnke. SSR-PCR and ABI3730XL-based fragment analysis were conducted by Sheron Simpson at the USDA-ARS, MSA Genomics Laboratory directed by Brian Scheffler. Amaresh Chandra gratefully acknowledges Department of Biotechnology, Government of India for DBT-CREST Fellowship Award. NR 54 TC 1 Z9 1 U1 3 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD FEB PY 2016 VL 207 IS 3 BP 527 EP 549 DI 10.1007/s10681-015-1538-5 PG 23 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CZ4WT UT WOS:000367104200005 ER PT J AU Li, T Zhang, DD Zhou, XL Bai, GH Li, L Gu, SL AF Li, Tao Zhang, Dadong Zhou, Xiali Bai, Guihua Li, Lei Gu, Shiliang TI Fusarium head blight resistance loci in a stratified population of wheat landraces and varieties SO EUPHYTICA LA English DT Article DE Allelic variation; Association analysis; Fusarium graminearum; Triticum aestivum; Wheat scab ID QUANTITATIVE TRAIT LOCI; X WANGSHUIBAI POPULATION; CULTIVAR SUMAI 3; COMPLEX TRAITS; WINTER-WHEAT; BREAD WHEAT; MAJOR QTL; BARLEY; LINES; POLYMORPHISM AB To determine if Chinese and Japanese wheat landraces and varieties have unique sources of Fusarium head blight (FHB) resistance, an association mapping panel of 195 wheat accessions including both commercial varieties and landraces was genotyped with 364 genome-wide simple sequence repeat and sequence-tagged site (STS) markers, and evaluated for type II FHB resistance in three greenhouse experiments using single floret inoculation. Population structure analysis stratified this population into five groups with Chinese landraces in four groups. Thirty-two of 51 Chinese landraces and 24 of 27 Japanese accessions were placed in one group. Association analysis using a mixed model identified 11 markers having significant associations with FHB resistance in at least two experiments. Most of these markers coincided with known quantitative trait loci (QTL) for FHB resistance with one potentially novel QTL associated with Xgdm138-5DS and Xgwm358-5DS. Xbarc19-3AS was significant in all three experiments, and the frequency of favorable alleles was more than 53 %. Chinese landraces and Japanese accessions had more favorable alleles at the majority of reproducible marker loci. Nine QTL combinations were identified according to the number of favorable alleles. Mean FHB severities increased with decreasing numbers of favorable alleles at reproducible loci. The resistance loci characterized here will further diversify the wheat FHB resistance gene pool, and provide breeders with additional sources of resistance for improvement of FHB resistance in wheat. C1 [Li, Tao; Zhou, Xiali; Li, Lei; Gu, Shiliang] Yangzhou Univ, Jiangsu Prov Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Yangzhou 225009, Jiangsu, Peoples R China. [Li, Tao; Zhou, Xiali; Li, Lei; Gu, Shiliang] Yangzhou Univ, Minist Educ, Key Lab Plant Funct Genom, Yangzhou 225009, Jiangsu, Peoples R China. [Li, Tao; Zhou, Xiali; Li, Lei; Gu, Shiliang] Yangzhou Univ, Wheat Res Ctr, Yangzhou 225009, Jiangsu, Peoples R China. [Zhang, Dadong; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Zhou, Xiali] Zhumadian Acad Agr Sci, Zhumadian 466000, Henan, Peoples R China. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Li, T (reprint author), Yangzhou Univ, Jiangsu Prov Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Yangzhou 225009, Jiangsu, Peoples R China. EM taoli@yzu.edu.cn FU National Major Project of Breeding for New Transgenic Organisms [2012ZX08009003-004]; NSFC [31171537]; Innovative Research Team of Universities in Jiangsu Province; PAPD FX We acknowledge financial support by the National Major Project of Breeding for New Transgenic Organisms (2012ZX08009003-004), NSFC (31171537), the Innovative Research Team of Universities in Jiangsu Province, and PAPD. Our thanks also go to Dr. Robert McIntosh (University of Sydney) for language improvement. NR 44 TC 0 Z9 1 U1 7 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD FEB PY 2016 VL 207 IS 3 BP 551 EP 561 DI 10.1007/s10681-015-1539-4 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CZ4WT UT WOS:000367104200006 ER PT J AU Islam, MS Brown-Guedira, G Van Sanford, D Ohm, H Dong, YH McKendry, AL AF Islam, Md. Sariful Brown-Guedira, Gina Van Sanford, David Ohm, Herb Dong, Yanhong McKendry, Anne L. TI Novel QTL associated with the Fusarium head blight resistance in Truman soft red winter wheat SO EUPHYTICA LA English DT Article DE QTL; Wheat; FHB; Truman ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; SPRING WHEAT; DEOXYNIVALENOL ACCUMULATION; MOLECULAR CHARACTERIZATION; WANGSHUIBAI POPULATION; FIELD-RESISTANCE; SCAB RESISTANCE; FUNGAL SPREAD; REGISTRATION AB Fusarium head blight (FHB) mainly caused by Fusarium graminearum Schwabe causes devastating losses in wheat globally. 'Truman' winter wheat, developed and released by the University of Missouri has excellent broad-based FHB resistance in a superior soft red winter wheat background. This research identified QTL associated with greenhouse type II resistance and field resistance for incidence, severity, Fusarium damaged kernels (FDK), and deoxynivalenol (DON) based on phenotypic data collected in Missouri, Kentucky and Indiana. Two years of replicated phenotypic data were collected on a set of 167 recombinant inbred lines. Genetic linkage maps were constructed using 160 SSR and 530 DArT polymorphic markers. Across years, QTL for type II resistance were identified on chromosomes 1BSc, 2BL, 2DS and 3BSc, for incidence on 2ASc, 2DS, and 3DS and for severity on 2DS and 3BSc. QTL were also detected for incidence on 1DLc and 2DS and for severity on 1BL, 3AL and 3BLC from data collected in Indiana and Kentucky, respectively. Common QTL for FDK on chromosomes 2ASc and 3BLc and for DON on chromosomes 2ASc and 2DS were identified from data from both Missouri and Kentucky, respectively with additional individual QTL for FDK and DON identified from tests at each independent location. All alleles were from Truman and associated with significant reductions in the respective traits. QTL on 2ASC, 2DS and 3DS may be novel and once further validated, should diversify the FHB gene pool globally and be useful for enhancing FHB resistance through marker assisted selection. C1 [Islam, Md. Sariful] USDA ARS, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. [Islam, Md. Sariful; McKendry, Anne L.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Brown-Guedira, Gina] N Carolina State Univ, Eastern Reg Small Grains Genotyping Lab, USDA ARS, Raleigh, NC 27695 USA. [Van Sanford, David] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Ohm, Herb] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Dong, Yanhong] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. RP Islam, MS (reprint author), USDA ARS, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM md.islam@ars.usda.gov FU U.S. Department of Agriculture [59-0206-9-077] FX The authors thank Dr. Georgia Davis at the University of Missouri and Dr. John Perry Gustafson of the Plant Genetics Unit of the USDA-ARS for use of their laboratory facilities during genotyping and analysis. This material is based upon work supported in part by the U.S. Department of Agriculture under Agreement No. 59-0206-9-077. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 78 TC 3 Z9 4 U1 8 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD FEB PY 2016 VL 207 IS 3 BP 571 EP 592 DI 10.1007/s10681-015-1550-9 PG 22 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CZ4WT UT WOS:000367104200008 ER PT J AU Lebeda, A Kristkova, E Sedlakova, B McCreight, JD Coffey, MD AF Lebeda, Ales Kristkova, Eva Sedlakova, Bozena McCreight, James D. Coffey, Michael D. TI Cucurbit powdery mildews: methodology for objective determination and denomination of races SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Golovinomyces orontii s.l.; Podosphaera xanthii; Cucumis melo; Virulence variability; Race-specificity; Septet code ID SPHAEROTHECA-FULIGINEA; CAUSAL AGENT; ERYSIPHE-CICHORACEARUM; SPECIES SPECTRUM; PLANT-PATHOGENS; MELON; RESISTANCE; PATHOTYPES; DIFFERENTIATION; IDENTIFICATION AB Cucurbit powdery mildew (CPM), a disease on field and greenhouse cucurbit crops worldwide, is caused most frequently by two obligate erysiphaceous ectoparasites (Golovinomyces orontii s.l., Podosphaera xanthii) that are highly variable in their pathogenicity and virulence. Various independent systems of CPM race determination and denomination are used worldwide, having to date been differentiated on different cultivars or lines of melon (Cucumis melo L.). We briefly review historical perspectives and the current state of understanding of the virulence variation of the two CPM pathogens at the pathogenic race level, their differentiation and their designation. Furthermore, we propose for use by the international CPM research, breeding, seed and production community new tools to enhance research, communication and management of CPM. These tools consist of five components: 1) a set of 21 differential genotypes of Cucumis melo L. for the identification of CPM races; 2) a triple-part, septet code for meaningful, concise designation of CPM races; 3) protocols for maintaining CPM isolates and differential genotypes and for laboratory assays to examine the virulence of CPM isolates; 4) rules and principles of practical application of this system in breeding, seed production and cucurbit growing, including a proposal of a race denomination suitable for practical application; and 5) crucial activities leading to the implementation and running of new tools for CPM research and management. The five components of this package have equal importance, forming a compact system, and none of them can be omitted. C1 [Lebeda, Ales; Kristkova, Eva; Sedlakova, Bozena] Palacky Univ, Dept Bot, Fac Sci, CR-77147 Olomouc, Czech Republic. [McCreight, James D.] ARS, USDA, Salinas, CA 93905 USA. [Coffey, Michael D.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. RP Lebeda, A (reprint author), Palacky Univ, Dept Bot, Fac Sci, Slechtitelu 11, CR-77147 Olomouc, Czech Republic. EM ales.lebeda@upol.cz FU Czech Ministry of Education [MSM 6198959215]; NAZV, National Agency for Agricultural Research of the Czech Republic [QH71229]; Internal Grant Agency Palacky University in Olomouc [Prf_2015_001] FX 1) MSM 6198959215 (Czech Ministry of Education); 2) QH71229 (NAZV, National Agency for Agricultural Research of the Czech Republic); 3) Internal Grant Agency Palacky University in Olomouc (Prf_2015_001). NR 79 TC 3 Z9 3 U1 15 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD FEB PY 2016 VL 144 IS 2 BP 399 EP 410 DI 10.1007/s10658-015-0776-7 PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CZ3HW UT WOS:000366996100015 ER PT J AU Keating, MP Ochs, CA Balachandran, D Holland, MM Lizotte, RE Yu, KW AF Keating, Michael P., Jr. Ochs, Clifford A. Balachandran, Devika Holland, Marjorie M. Lizotte, Richard E., Jr. Yu, Kewei TI Spatial variation related to hydrologic patterns and vegetation in greenhouse gas fluxes from the Mississippi Delta agricultural region SO APPLIED SOIL ECOLOGY LA English DT Article DE Greenhouse gas; Agriculture; Mississippi Delta; Denitrification; Vegetation; nosZ gene ID CREATED RIPARIAN MARSHES; METHANE EMISSIONS; N2O; SOIL; DENITRIFICATION; CH4; NITRIFICATION; WETLANDS; CO2; INTERFACE AB Analysis of microbial gas emissions collected with field chambers were combined with soil genetic and chemical analyses to determine hotspots of greenhouse gas emissions from vegetated cropland, drainage ditch, riparian zone, and wetland habitats in the Tunica and Sunflower Counties of the north Mississippi Delta agricultural region. Methane (CH4) emissions were highest in sites with highest soil moisture (wetlands) and low water flow rates, whereas carbon dioxide (CO2) emission peaked in sites with intermediate soil moisture (riparian zone). Nitrous oxide (N2O) emissions were highest in the cropland and riparian zones, although soil genetic and chemical data suggest a difference in N2O sources between these two sites. Denitrification gene (nosZ) abundances indicate denitrification-derived N2O products in intermittently-flooded sites, while soil [NO3 +NO2] concentrations suggest nitrification-derived N2O products in dry cultivated sites. Vegetation reduced gas emissions in riparian zone habitat, but had no effect in wetland habitat. Substantial and consistent differences in gas emission rates between two neighboring wetlands corresponded to differences in flow regime. Wetland hydrologic flow regime is the factor regulating the distinct gas emission differences observed between two neighboring wetlands. This study demonstrates the importance of hydrologic factors and location-specific vegetation on gas emission hotspots in this intensively-managed agricultural landscape. (C) 2015 Elsevier B.V. All rights reserved. C1 [Keating, Michael P., Jr.; Ochs, Clifford A.; Holland, Marjorie M.] Univ Mississippi, Dept Biol, University, MS 38677 USA. [Balachandran, Devika] Princeton Univ, Princeton Environm Inst, Princeton, NJ 08544 USA. [Lizotte, Richard E., Jr.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Yu, Kewei] Troy Univ, Dept Biol & Environm Sci, Troy, AL 36082 USA. RP Keating, MP (reprint author), Mississippi State Univ, Dept Landscape Architecture, 845-3 Stone Blvd, Mississippi State, MS 39762 USA. EM mpk76@msstate.edu FU USDA-Agricultural Research Service [58-6408-1-595]; University of Mississippi Graduate School and Biology Department FX This project was supported financially by the USDA-Agricultural Research Service (Award #: 58-6408-1-595) and the University of Mississippi Graduate School and Biology Department. We acknowledge essential contributions from the Princeton University Environmental Institute, the USDA-Agricultural Research Service, the University of Mississippi National Center for Natural Products Research-Thad Cochran Research Center, L. Hedin, D. Hoyos, J. O'Haver, and]. Rimoldi. R.W. Steinriede provided valuable land-use information for Beasley Lake watershed. J. Murdock and J. Hoeksema provided important advice on project design and statistical analysis. The laboratory of C. Jackson was instrumental in genetic analyses. Finally, A. Myers was very supportive in allowing this research to be carried out on his farm. NR 24 TC 0 Z9 0 U1 9 U2 54 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD FEB PY 2016 VL 98 BP 278 EP 281 DI 10.1016/j.apsoil.2015.09.012 PG 4 WC Soil Science SC Agriculture GA CX9AW UT WOS:000365998100030 ER PT J AU Williams, MI Dumroese, RK Page-Dumroese, DS Hardegree, SP AF Williams, Mary I. Dumroese, R. Kasten Page-Dumroese, Deborah S. Hardegree, Stuart P. TI Can biochar be used as a seed coating to improve native plant germination and growth in arid conditions? SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Plant establishment; Restoration; Temperature; Water potential ID SOIL; RESTORATION; TEMPERATURE; PRODUCTIVITY; HYDROCARBONS; TECHNOLOGIES; METAANALYSIS; FERTILITY; BLACK AB Direct seeding is a common large-scale restoration practice for revegetating arid and semi-arid lands, but success can be limited by moisture and temperature. Seed coating technologies that use biochar may have the potential to overcome moisture and temperature limitations on native plant germination and growth. Biochar is a popular agronomic tool for improving soil properties, such as water availability and nutrient retention and has been recently marketed, but not tested, as a seed coating. We analyzed the effect of biochar seed coating thicknesses on the germination and growth of four plant species native to western United States: mountain brome (Bromus marginatus), prairie junegrasss (Koeleria cristata), Wyeth's buckwheat (Eriogonum heracleoides), and western yarrow (Achillea millefolium). Across different temperature and water potential treatments using environmental chambers and polyethylene glycol (PEG) solutions, biochar coating applied at different thicknesses had either a neutral or negative effect on germination for all species. In the field, biochar seed coatings slightly improved mountain brome root weight and prairie junegrass cover. Our results, alongside the high economic expense of native plant seed and direct seeding operations, suggest that biochar, by itself, may not be an appropriate seed coating for improving native plant establishment. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Williams, Mary I.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Dumroese, R. Kasten; Page-Dumroese, Deborah S.] USDA Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Hardegree, Stuart P.] USDA ARS, Boise, ID 83712 USA. RP Williams, MI (reprint author), 2824 S Devereaux Way, Salt Lake City, UT 84109 USA. EM miwillia@mtu.edu; kdumroese@fs.fed.us; ddumroese@fs.fed.us; stuart.hardegree@usda.ars.gov FU U.S. Department of Agriculture (U.S. Forest Service National Center for Reforestation, Nurseries, and Genetics Resources); U.S. Department of Agriculture (Rocky Mountain Research Station); U.S. Department of Agriculture (Agricultural Research Service); Western Forestry and Conservation Association; Michigan Technological University FX We thank Jonah Levine (Biochar Now) for biochar processing and Matthew Ineck (Summit Seed Coatings) and Howard Boyte (Walking Point Farms) for coating the seeds. We thank these U.S. Forest Service employees: Jim Archuleta (Region 6) for coordinating the seed coating process; Aram Eramian and Brian Flynn (Coeur d'Alene Nursery) for field work; Kayla Herriman and her staff (Bend Pine Seed Extractory) for seed cleaning; and Victor Vankus (National Seed Laboratory) for seed quality testing. We thank Robin Bjork, Alex Boehm, Maren Watkins, Hailey Youngling, Jan Gurr, Eric Doubet, Tela Barkley, and Peng Zhang for field and laboratory work and Amy Ross-Davis for assistance with data analysis and interpretation. Financial and technical support was provided by the U.S. Department of Agriculture (U.S. Forest Service National Center for Reforestation, Nurseries, and Genetics Resources; Rocky Mountain Research Station; and Agricultural Research Service), Western Forestry and Conservation Association, and Michigan Technological University. The views expressed are strictly those of the authors and do not represent the positions or policy of their respective institutions. NR 44 TC 1 Z9 1 U1 4 U2 51 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD FEB PY 2016 VL 125 BP 8 EP 15 DI 10.1016/j.jaridenv.2015.09.011 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CZ0CP UT WOS:000366773000002 ER PT J AU Liu, SX Palti, Y Gao, GT Rexroad, CE AF Liu, Sixin Palti, Yniv Gao, Guangtu Rexroad, Caird E., III TI Development and validation of a SNP panel for parentage assignment in rainbow trout SO AQUACULTURE LA English DT Article DE Parentage assignment; SNP; Rainbow trout; Fluidigm; Pedigree; Aquaculture ID SINGLE-NUCLEOTIDE POLYMORPHISMS; FAMILY GROWTH-RESPONSE; ONCORHYNCHUS-MYKISS; FISH-MEAL; IDENTIFICATION; MICROSATELLITES; MARKERS; POPULATIONS; STEELHEAD; EXCLUSION AB In salmonid aquaculture, family-based selective breeding programs rely on accurate pedigree information for estimating genetic merits. The pedigree information can be tracked through the use of Passive Integrated Transponder (PIT) tags. However, PIT tags can be expensive, and cannot be used with small fish. DNA markers offer an alternative approach for pedigree tracking and microsatellites have been previously used for parentage assignments in rainbow trout. Single nucleotide polymorphisms (SNPs) are becoming increasingly popular in aquaculture research because they are highly abundant and are amenable for high throughput genotyping. The objectives of this study were to develop a SNP panel for parentage assignment in rainbow trout and to evaluate the accuracy of parentage assignment using fish with known pedigrees. Two groups of SNPs were targeted: 1) genome SNPs distributed across all 29 chromosomes; and 2) previously identified trait-associated SNPs. Among the 128 SNPs selected for assay development, 102 (79.7%) working assays were developed. A panel of 95 SNP assays including 68 genome SNPs and 27 trait-associated SNPs was used to genotype 499 fish with known pedigrees from Troutlodge, Inc. and from the USDA/ARS National Center for Cool and Cold Water Aquaculture. Parentage assignments matched perfectly with the known pedigrees when all 95 SNPs were used. Near perfect parentage assignment was obtained with the 68 genome SNPs, and the accuracy of parentage assignment was over 98% with a subset of 48 genome SNPs. Therefore, these SNP assays are robust and enable accurate parentage analysis in populations that are typically used in rainbow trout breeding programs. Statement of relevance: Provide alternative method to track pedigrees. Published by Elsevier B.V. C1 [Liu, Sixin; Palti, Yniv; Gao, Guangtu; Rexroad, Caird E., III] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. RP Liu, SX (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM sixin.liu@ars.usda.gov FU Agricultural Research Service CRIS Project "Integrated Research Approaches for Improving Production Efficiency in Salmonids" [1930-31000-012] FX We would like to thank Roseanna Long and Kristy Shewbridge for their technical assistance, and Dr. Kyle Martin for providing fin clips of fish from the Troutlodge population. We are also grateful to Dr. John Buchanan for his critical review of the manuscript. This work was supported by Agricultural Research Service CRIS Project 1930-31000-012 "Integrated Research Approaches for Improving Production Efficiency in Salmonids". Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 31 TC 3 Z9 3 U1 10 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD FEB 1 PY 2016 VL 452 BP 178 EP 182 DI 10.1016/j.aquaculture.2015.11.001 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CY1YY UT WOS:000366205900022 ER PT J AU Koch, JF Rawles, SD Webster, CD Cummins, V Kobayashi, Y Thompson, KR Gannam, AL Twibell, RG Hyde, NM AF Koch, Joao Fernando Rawles, Steven D. Webster, Carl D. Cummins, Vaun Kobayashi, Yuka Thompson, Kenneth R. Gannam, Ann L. Twibell, Ronald G. Hyde, Nathan M. TI Optimizing fish meal-free commercial diets for Nile tilapia, Oreochromis niloticus SO AQUACULTURE LA English DT Article DE Tilapia; Fish meal-free diets; Amino acids; Ideal protein formulation; Taurine supplementation ID IDEAL PROTEIN CONCEPT; CRAYFISH CHERAX-QUADRICARINATUS; AMINO-ACID AVAILABILITY; FED PRACTICAL DIETS; FEED INGREDIENTS; APPARENT DIGESTIBILITY; TOTAL REPLACEMENT; SOYBEAN-MEAL; BODY-COMPOSITION; ATLANTIC SALMON AB A feeding trial was conducted in a closed recirculating aquaculture system with Nile tilapia Oreochromis niloticus juveniles (mean weight, 6.81 g) to examine the response to a practical diet containing protein primarily from menhaden fish meal (FM) and soybean meal (SBM) (control, Diet 1) or to diets with decreasing ratios of PBM to SBM (Diets 2-7; dose-response) as a total replacement for digestible protein from FM, and the efficacy of 1% supplemental taurine (Tau) at the highest level of plant protein inclusion by removing Tau (Diet 8). To the extent possible, the replacement diets were formulated using currently published amino acid availabilities for the ingredients of interest in order to estimate and supplement the first two limiting amino acids (Met and Lys) to match levels in the FM control diet. The test diets were formulated to contain 35% digestible protein. Fish were fed three times daily all they would consume in 30 min. All performance measures were quadratic with respect to PBM: SBM ratio in the diet. The highest weight gain, lowest average daily feed intake, lowest feed conversion, and greatest specific growth rate coincided with a dietary PBM: SBM ratio of 1.22 to 1.35 suggesting that the best tilapia performance in the current trial was achieved with replacement formula D3 that contained approximately 20% SBM, 30% PBM, and supplemental Lys, Met, and Tau. However, all growth performance measures were significantly linear and decreased with respect to increasing distance from the ideal protein amino acid profile for tilapia. Positive effects of taurine supplementation at the highest level of dietary plant protein inclusion were not observed and may have been overwhelmed by imbalances in other amino acids in the test diets. The current results provide evidence that total deviation from the ideal protein profile in tilapia is an important consideration for diet formulation when combinations of diet ingredients are used. Hence, the essential amino acid content of a fish meal control diet may be an inadequate target for optimizing fish meal replacement diets for tilapia; whereas the whole body or muscle amino acid pattern may be a more useful formulation target. Finally, while the database of ingredients that have been evaluated in tilapia is growing, the industry will benefit from more efficient diets as long-term averages of amino acid composition and digestibility accrue for a variety of traditional and novel ingredients. Statement of Relevance: The current results provide evidence that it is the total deviations from a postulated ideal protein profile that is a more important consideration for diet formulation than the combination of diet ingredients used to meet that profile. Therefore, it should be possible to formulate least-cost fish meal replacement diets for tilapia, irrespective of ingredient combinations, and diet intact protein level, as long as a reasonable amino acid model is chosen and a fairly robust set of ingredient composition and digestibility data are available. Published by Elsevier B.V. C1 [Koch, Joao Fernando] Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, BR-18618000 Sao Paulo, Brazil. [Rawles, Steven D.; Webster, Carl D.] ARS, USDA, HKDSNARC, Stuttgart, AR 72160 USA. [Cummins, Vaun; Kobayashi, Yuka; Thompson, Kenneth R.] KSU, Aquaculture Res Ctr, Frankfort, KY 40601 USA. [Gannam, Ann L.; Twibell, Ronald G.; Hyde, Nathan M.] US Fish & Wildlife Serv, Abernathy Fish Technol Ctr, Longview, WA 98632 USA. RP Rawles, SD (reprint author), ARS, USDA, HKDSNARC, POB 1050,2955 Hwy 130 E, Stuttgart, AR 72160 USA. EM steven.rawles@ars.usda.gov; Carl.Webster@ars.usda.gov FU USDA grant [KYX-80-09-18A]; USDA/ARS CRIS project [6028-31630-006-00D]; U.S. Department of Agriculture FX The authors thank N. Ann, K.C., K.N. Dee, B.R. Lee, T.I.L. Lee, E.M. Maa, Cathy Rhin, B. Rett, Shi Ron, M.S. Tee, D.R. Wynne, and Sam Wise for technical assistance and T. Gibson Gaylord, Margarida Barros, and Wilson Furuya for their constructive comments during the development of this manuscript. This research project was partially funded by a USDA grant under agreement KYX-80-09-18A to Kentucky State University and by USDA/ARS CRIS project 6028-31630-006-00D Developing Nutritional, Genetic, and Management Strategies to Enhance Warmwater Finfish Production. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 47 TC 0 Z9 0 U1 6 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD FEB 1 PY 2016 VL 452 BP 357 EP 366 DI 10.1016/j.aquaculture.2015.11.017 PG 10 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CY1YY UT WOS:000366205900044 ER PT J AU Lujaji, FC Boateng, AA Schaffer, M Mtui, PL Mkilaha, ISN AF Lujaji, Frank C. Boateng, Akwasi A. Schaffer, Mark Mtui, Peter L. Mkilaha, Iddi S. N. TI Spray atomization of bio-oil/ethanol blends with externally mixed nozzles SO EXPERIMENTAL THERMAL AND FLUID SCIENCE LA English DT Article DE Biomass; Pyrolysis oil; Spray atomization ID BIOMASS PYROLYSIS; COMBUSTION PERFORMANCE; OIL PRODUCTION; DIESEL-ENGINE; SWIRL BURNER; FUELS; BIODIESEL; EMISSIONS; PRESSURE; LIQUIDS AB Experiments were conducted to investigate the properties of sprays of pyrolysis oil (bio-oil) blends with ethanol using an air assisted atomization nozzle operated without combustion. This was done in order to explore the potential for pyrolysis oil combustion in industrial and residential furnaces. The liquid samples investigated were bio-oil blends with ethanol (EtOH), neat ethanol and diesel. The bio-oil:EtOH blends were prepared in concentrations of 20:80 and 40:60 vol%. Twin-fluid externally mixed nozzles SU2, SU4, and SU5 with liquid orifice areas of 0.40, 1.82 and 5.07 mm(2), respectively, were used in the spray experiments. The liquid and atomizing air flow rates as well as temperature were controlled to maintain constant liquid flow rates (cc/s) equivalent to 30 and 50 kW energy input. Images of atomized spray droplets were measured to determine their size and velocity. Results show that it is possible to spray bio-oil/ethanol mixtures containing up to 40% bio-oil that has a low water content (12.60%). High viscosity and a tendency to coagulate were the main drawbacks; however, the 20:80 bio-oil: EtOH blend and neat ethanol in all three nozzles exhibited spray characteristics similar to that of diesel over atomization air flow rates of 15-30 SLPM. Published by Elsevier Inc. C1 [Lujaji, Frank C.; Boateng, Akwasi A.; Schaffer, Mark] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Lujaji, Frank C.; Mtui, Peter L.; Mkilaha, Iddi S. N.] Nelson Mandela African Inst Sci & Technol, Dept Mat & Energy Sci & Engn, Arusha, Tanzania. RP Boateng, AA (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov OI Schaffer, Mark/0000-0003-3990-9492 FU Tanzania Commission for Science and Technology (COSTECH); Biomass Research & Development Initiative (BRDI) Competitive Grant from the USDA National Institute of Food and Agriculture (NIFA) [2012-10008-20271] FX The authors would like to thank Mr. Neil Goldberg for assistance in constructing the spray test rig, Dr. Charles Mullen for pyrolysis oil production, and Mr. Craig Einfeldt for his assistance during the spray tests. The scholarship by Tanzania Commission for Science and Technology (COSTECH) for PhD studies at Nelson Mandela African Institution of Science and Technology (NM-AIST) is acknowledged. This project was supported by Biomass Research & Development Initiative (BRDI) Competitive Grant No. 2012-10008-20271 from the USDA National Institute of Food and Agriculture (NIFA). NR 33 TC 3 Z9 3 U1 7 U2 26 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0894-1777 EI 1879-2286 J9 EXP THERM FLUID SCI JI Exp. Therm. Fluid Sci. PD FEB PY 2016 VL 71 BP 146 EP 153 DI 10.1016/j.expthermflusci.2015.10.020 PG 8 WC Thermodynamics; Engineering, Mechanical; Physics, Fluids & Plasmas SC Thermodynamics; Engineering; Physics GA CY5JA UT WOS:000366442700015 ER PT J AU Hossain, F Follett, P Vu, KD Harich, M Salmieri, S Lacroix, M AF Hossain, Farah Follett, Peter Khang Dang Vu Harich, Mehdi Salmieri, Stephane Lacroix, Monique TI Evidence for synergistic activity of plant-derived essential oils against fungal pathogens of food SO FOOD MICROBIOLOGY LA English DT Article DE Essential oil; Minimum inhibitory concentration (MIC); Fractional inhibitory concentration (FIC); Fungal growth parameter ID ENHANCED ANTIMICROBIAL ACTIVITY; STARCH EDIBLE FILMS; ANTIFUNGAL ACTIVITY; ASPERGILLUS-FLAVUS; BACILLUS-CEREUS; MEDICINAL-PLANT; ACID NANOGEL; VULGARE L.; IN-VITRO; CHITOSAN AB The antifungal activities of eight essential oils (EOs) namely basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree and thyme were evaluated for their ability to inhibit growth of Aspergillus niger, Aspergillus flavus, Aspergillus parasiticus and Penicillium chrysogenum. The antifungal activity of the EOs was assessed by the minimum inhibitory concentration (MIC) using 96-well microplate analysis. The interactions between different EO combinations were done by the checkerboard technique. The highest antifungal activity was exhibited by oregano and thyme which showed lower MIC values amongst all the tested fungi. The antifungal activity of the other EOs could be appropriately ranked in a descending sequence of cinnamon, peppermint, tea tree and basil. Eucalyptus and mandarin showed the least efficiency as they could not inhibit any of the fungal growth at 10,000 ppm. The interaction between these two EOs also showed no interaction on the tested species. A combined formulation of oregano and thyme resulted in a synergistic effect, showing enhanced efficiency against A. flavus and A. parasiticus and P. chrysogenum. Mixtures of peppermint and tea tree produced synergistic effect against A. niger. Application of a modified Gompertz model considering fungal growth parameters like maximum colony diameter, maximum growth rate and lag time periods, under the various EO treatment scenarios, showed that the model could adequately describe and predict the growth of the tested fungi under these conditions. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Hossain, Farah; Khang Dang Vu; Harich, Mehdi; Salmieri, Stephane; Lacroix, Monique] Univ Quebec, Inst Armand Frappier, INRS, Canadian Irradiat Ctr,Res Labs Sci Appl Food, Laval, PQ H7V 1B7, Canada. [Follett, Peter] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Lacroix, M (reprint author), Univ Quebec, Inst Armand Frappier, INRS, Canadian Irradiat Ctr,Res Labs Sci Appl Food, 531 Blvd Prairies, Laval, PQ H7V 1B7, Canada. EM monique.lacroix@iaf.inrs.ca FU United States Department of Agriculture [58-5320-2-097F, 58-5320-3-019F]; Natural Sciences and Engineering Research Council of Canada (NSERC)-Discovery Programme [155141-2009] FX This research was supported by the United States Department of Agriculture (58-5320-2-097F and 58-5320-3-019F) and the Natural Sciences and Engineering Research Council of Canada (NSERC)-Discovery Programme (155141-2009). The authors are also thankful to Ministere de l'Economie, de l'Innovation et des Exportations, Quebec. NR 32 TC 7 Z9 7 U1 4 U2 35 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD FEB PY 2016 VL 53 BP 24 EP 30 DI 10.1016/j.fm.2015.08.006 PN B PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CX9XX UT WOS:000366061200004 PM 26678126 ER PT J AU Miks-Krajnik, M Yoon, YJ Ukuku, DO Yuk, HG AF Miks-Krajnik, Marta Yoon, Yong-Jin Ukuku, Dike O. Yuk, Hyun-Gyun TI Volatile chemical spoilage indexes of raw Atlantic salmon (Salmo salar) stored under aerobic condition in relation to microbiological and sensory shelf lives SO FOOD MICROBIOLOGY LA English DT Article DE Pseudomonas spp.; Shelf life; Specific spoilage organism; Salmon; Volatile organic compounds; Spoilage indicators ID COLD-SMOKED SALMON; SOLID-PHASE MICROEXTRACTION; SPME-GC-MS; ONCORHYNCHUS-GORBUSCHA; GAS-CHROMATOGRAPHY; MEAT SPOILAGE; BACTERIA; STORAGE; FISH; QUALITY AB The purpose of this investigation was to identify and quantify the volatile chemical spoilage indexes (CSIs) for raw Atlantic salmon (Salmo salar) fillets stored under aerobic storage conditions at 4, 10 and 21 degrees C in relation to microbial and sensory shelf lives. The volatile organic compounds (VOCs) were analyzed with SPME-GC-MS technique. Through multivariate chemometric method, hierarchical cluster analysis (HCA) and Pearson's correlations, the CSIs: trimethylamine (TMA), ethanol (EtOH), 3-methyl-1-butanol (3Met-1But), acetoin and acetic acid (C-2) were selected from the group of 28 detected VOCs. At the moment of microbiological shelf life established at total viable count (TVC) of 7.0 log CFU/g, the CSIs achieved levels of 11.5, 38.3, 0.3, 24.0 and 90.7 mu g/g of salmon for TMA, EtOH, 3M-1But, acetoin and C-2, respectively. Pseudomonas spp. was found as major specific spoilage organism (SSOs), suitable for shelf life prediction using modified Gompertz model at the cut-off level of 6.5 log CFU/g. H2S producing bacteria and Brochothrix thermosphacta were considered as important spoilage microorganisms; however, they were not suitable for shelf life estimation. Partial least square (PLS) regression revealed possible associations between microorganisms and synthetized VOCs, showing correlations between Pseudomonas spp. and 3Met-1But and aldehydes synthesis, lactic acid bacteria were linked with EtOH, C-2 and esters, and B. thermosphacta with acetoin formation. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Miks-Krajnik, Marta; Yuk, Hyun-Gyun] Natl Univ Singapore, Dept Chem, Food Sci & Technol Programme, Singapore 117543, Singapore. [Yuk, Hyun-Gyun] Natl Univ Singapore, Suzhou Res Inst, Suzhou 215123, Jiangsu, Peoples R China. [Yoon, Yong-Jin] Nanyang Technol Univ, Sch Mech & Aerosp Engn, Div Engn Mech, Singapore 639798, Singapore. [Ukuku, Dike O.] ARS, Food Safety Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Yuk, HG (reprint author), Natl Univ Singapore, Dept Chem, Food Sci & Technol Programme, 3 Sci Dr 3, Singapore 117543, Singapore. EM chmyukhg@nus.edu.sg RI Yoon, Yong-Jin/A-3860-2011; OI Yoon, Yong-Jin/0000-0002-3885-4947; Yuk, Hyun-Gyun/0000-0001-9841-7899 FU A*STAR/Science & Engineering Research Council - SERC research project [112 177 0039/R-143-000-511-305] FX This study was financially supported by A*STAR/Science & Engineering Research Council - SERC research project (grant no. 112 177 0039/R-143-000-511-305). NR 54 TC 4 Z9 5 U1 12 U2 50 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD FEB PY 2016 VL 53 BP 182 EP 191 DI 10.1016/j.fm.2015.10.001 PN B PG 10 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CX9XX UT WOS:000366061200024 PM 26678146 ER PT J AU Zhang, XJ Fan, XT Solaiman, DKY Ashby, RD Liu, ZS Mukhopadhyay, S Yan, RX AF Zhang, Xuejie Fan, Xuetong Solaiman, Daniel K. Y. Ashby, Richard D. Liu, Zengshe Mukhopadhyay, Sudarsan Yan, Ruixiang TI Inactivation of Escherichia coli O157:H7 in vitro and on the surface of spinach leaves by biobased antimicrobial surfactants SO FOOD CONTROL LA English DT Article DE Biosurfactant; Sophorolipid; Thiamine dilauryl sulfate; Escherichia coli; Spinach ID RING-OPENING POLYMERIZATION; EPOXIDIZED SOYBEAN OIL; LISTERIA-MONOCYTOGENES; ACTIVE PROPERTIES; FRESH PRODUCE; LIGHT PL; ACID; LETTUCE; SANITIZER; WATER AB This study was conducted to evaluate the effect of biosurfactants on the populations of Escherichia coli O157:H7 in suspension and on spinach leaves. Eight surfactants including four soybean oil-based biosurfactants, sodium dodecyl sulfate (SDS), polyoxyethylene sorbitan monooleate (Tween 80), sophorolipid (SO) and thiamine dilauryl sulfate (TDS) at concentrations of 0.1%, 0.5% and 1.0% were tested in bacterial suspension, and the most effective biosurfactants were applied on spinach leaves. Results showed that the soybean oil-based biosurfactants, SDS or Tween 80 did not significantly affect E. coli O157:H7 populations. SO and TDS at concentrations of 1.0% were effective in reducing E. coli O157:H7 populations in bacterial suspension. E. coli O157:H7 with an initial population of 7.1 log CFU/mL was not detectable (detection limit: 1 log CFU/mL) after 1 min in 1.0% TDS or after 2 h in 1.0% SO. On spinach leaves, SO at 1% did not significantly affect E. coli when compared to a water wash during 7 days post-treatment storage at 4 degrees C. However, TDS (1.0%) wash was as effective as 200 ppm chlorine in reducing population of spot inoculated E. coli O157:H7, achieving 3.1 and 2.7 log CFU/per leaf at day 0, and 1.4 and 1.9 log CFU/leaf at day 7 when compared with a water wash. No apparent change in spinach visual quality was observed. None of treatments caused changes in visual quality of spinach. Electron micrographs suggested ultrastructural damage of bacterial cells such as separation of the outer membrane from the cytoplasmic membrane. Overall, our results showed that SO and TDS may be potential sanitizers in inactivating human pathogens such as E. coli O157:H7 in wash water and on fresh produce. Published by Elsevier Ltd. C1 [Zhang, Xuejie; Yan, Ruixiang] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China. [Zhang, Xuejie; Fan, Xuetong; Solaiman, Daniel K. Y.; Ashby, Richard D.; Mukhopadhyay, Sudarsan] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Liu, Zengshe] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Yan, Ruixiang] Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin 300384, Peoples R China. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov FU Monitoring Technology on Quality and safety of fresh agro-products [2012BAD38B05]; 'Key laboratory of Biology and GeneticImprovement of Horticultural Crops', Ministry of Agriculture, P. R. China FX This study was supported by national project 'Monitoring Technology on Quality and safety of fresh agro-products' 2012BAD38B05 and 'Key laboratory of Biology and GeneticImprovement of Horticultural Crops', Ministry of Agriculture, P. R. China to Xuejing Zhang. We thank Kanematsu U.S.A. Inc. for providing the TDS, Kim Sokorai for technical assistance, and Joseph Uknalis for SEM imaging. NR 46 TC 3 Z9 3 U1 4 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD FEB PY 2016 VL 60 BP 158 EP 165 DI 10.1016/j.foodcont.2015.07.026 PG 8 WC Food Science & Technology SC Food Science & Technology GA CW3HP UT WOS:000364882900021 ER PT J AU Yu, QQ Niu, MY Yu, MQ Liu, YH Wang, DP Shi, XM AF Yu, Qianqian Niu, Mengya Yu, Mengquan Liu, Yanhong Wang, Dapeng Shi, Xianming TI Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shellfish in Shanghai SO FOOD CONTROL LA English DT Article DE Vibrio parahaemolyticus; Antimicrobial resistance; Shellfish; Total presumptive Vibrio parahaemolyticus counts ID THERMOSTABLE DIRECT HEMOLYSIN; ANTIBIOTIC-RESISTANCE; PATHOGENIC BACTERIA; BIVALVE SHELLFISH; SOUTH-CAROLINA; OYSTER TISSUES; ANIMAL HEALTH; FOOD SAFETY; AQUACULTURE; SHRIMP AB Vibrio parahaemolyticus is a marine and estuarine bacterium that poses the greatest threat to human health worldwide. It has been the leading bacterial cause of seafood-borne illness. This study investigated the prevalence and drug resistance of V parahaemolyticus isolated from retail shellfish in Shanghai. A total of 140 shellfish samples were collected from February 2014 to February 2015. The occurrence of V. parahaemolyticus in shellfish was 34.3%, which has increased compared to previous reports. In addition, discernible differences of total presumptive V parahaemolyticus counts (TPVPC) were also observed in shellfish between market A and B. The results from PCR assays indicated that thermostable direct hemolysin (tdh) gene was positive in two isolates (2.1%), and the thermostable direct hemolysin-related hemolysin (trh) gene was not detected in all isolates. Antibiotic resistance profiles of those isolates were as follows: ampicillin (87.5%), cephazolin (31.3%), cephalothin (6.3%), amoxicillin/clavulanic acid (6.3%), piperacillin (6.3%), and amikacin (3.2%). Thirty-three out of 96 isolates were resistant to two or more antimicrobial agents. It is suggested that V parahaemolyticus in retail shellfish could be a potential risk to consumers in Shanghai. (c) 2015 Published by Elsevier Ltd. C1 [Yu, Qianqian; Niu, Mengya; Yu, Mengquan; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Sch Agr & Biol, Shanghai 200240, Peoples R China. [Yu, Qianqian; Niu, Mengya; Yu, Mengquan; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China. [Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens Res Unit, ERRC, USDA, Wyndmoor, PA 19038 USA. RP Shi, XM (reprint author), Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Sch Agr & Biol, Shanghai 200240, Peoples R China. EM norovirus@163.com; xmshi@sjtu.edu.cn FU Ministry of Science and Technology of China [2012AA101601]; National Natural Science Foundation of China [31000063] FX This work was jointly supported by the grant No. 2012AA101601 from the Ministry of Science and Technology of China and the grant No. 31000063 from the National Natural Science Foundation of China. NR 56 TC 2 Z9 2 U1 5 U2 27 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD FEB PY 2016 VL 60 BP 263 EP 268 DI 10.1016/j.foodcont.2015.08.005 PG 6 WC Food Science & Technology SC Food Science & Technology GA CW3HP UT WOS:000364882900035 ER PT J AU Hong, YK Huang, LH Yoon, WB AF Hong, Yoon-Ki Huang, Lihan Yoon, Won Byong TI Mathematical modeling and growth kinetics of Clostridium sporogenes in cooked beef SO FOOD CONTROL LA English DT Article DE Clostridium sporogenes; Growth kinetics; Modeling ID SODIUM-CHLORIDE; BOTULINUM; SPORES; MICROBIOLOGY; TEMPERATURES; OPTIMIZATION; SURROGATE AB Clostridium sporogenes PA 3679 is a common surrogate for proteolytic Clostridium botulinum for thermal process development and validation. However, little information is available concerning the growth kinetics of C. sporogenes in food. Therefore, the objective of this study was to investigate the growth kinetics of C. sporogenes in cooked beef under different temperature conditions. Ground beef samples, inoculated with C. sporogenes spores, were incubated at temperatures between 8 and 47 degrees C to examine the growth of this microorganism. Two primary models (Huang and Baranyi models) were used to analyze the growth data. The Ratkowsky square-root model was used as the secondary model to evaluate the effect of temperature on bacterial growth rate and lag time. The USDA IPMP 2013, a free data analysis tool for predictive microbiology, was used in data analysis. No growth of C. sporogenes was observed at temperatures below 15 degrees C for up to 25 days. At temperatures between 20 and 47 degrees C, C. sporogenes grew in cooked beef. The growth curves could be analyzed by both Huang and Baranyi models. The root mean squared error (RMSE) was 0.375 for the Huang model, and 0.441 for the Baranyi model with a global 110 of 10.46. The nominal minimum growth temperature (T-0) estimated from the Huang model was 15.5 degrees C, which was 12.7 degrees C for the Baranyi model. The maximum growth temperature was 48.0 and 48.3 degrees C for the Baranyi and Huang models, respectively. The lag times and specific growth rates of C. sporogenes observed in this study were reasonably close to the data reported in the literature for C. botulinum under comparable conditions, suggesting that C. sporogenes may be used as a potential surrogate for evaluating the growth of C. botulinum in cooked meats during cooling. However, a direct comparison of growth kinetics between the two microorganisms is needed to confirm the suitability of C. sporogenes as a surrogate of C. botulinum. Published by Elsevier Ltd. C1 [Huang, Lihan] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Hong, Yoon-Ki; Yoon, Won Byong] Kangwon Natl Univ, Dept Bioconvers Sci & Technol, Chunchon 200701, Gangwon, South Korea. [Hong, Yoon-Ki; Yoon, Won Byong] Kangwon Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Biotechnol, Chunchon 200701, Gangwon, South Korea. RP Huang, LH (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov OI Huang, Lihan/0000-0002-9565-4503 NR 24 TC 0 Z9 1 U1 11 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD FEB PY 2016 VL 60 BP 471 EP 477 DI 10.1016/j.foodcont.2015.08.035 PG 7 WC Food Science & Technology SC Food Science & Technology GA CW3HP UT WOS:000364882900063 ER PT J AU Weng, SC Luo, YG Li, J Zhou, B Jacangelo, JG Schwab, KJ AF Weng, ShihChi Luo, Yaguang Li, Jie Zhou, Bin Jacangelo, Joseph G. Schwab, Kellogg J. TI Assessment and speciation of chlorine demand in fresh-cut produce wash water SO FOOD CONTROL LA English DT Article DE Fresh-cut produce; Wash water; Chlorination; Chlorine demand ID SIZE-EXCLUSION CHROMATOGRAPHY; NATURAL ORGANIC-MATTER; CROSS-CONTAMINATION; PROCESSING-INDUSTRY; FOOD-INDUSTRY; INACTIVATION; LETTUCE; OUTBREAK; SALMONELLA; REUSE AB For the fresh-cut produce industry, a critical area of concern is potential pathogen cross-contamination during wash operations when wash water is reused and re-circulated in wash systems continuously imputed with fresh-cut produce. However, little research has focused on the chemical properties of wash water. Organic input from residual soil and vegetable material deteriorates water quality and creates increasing chlorine demand within this wash water. This study evaluated the origins of chlorine demand input and chlorine decay kinetics of fresh-cut produce wash water. Using a model system, vegetable juice released per kg of processed produce for shredded romaine lettuce, shredded iceberg lettuce, shredded carrot and baby spinach was 82.1 mL/kg, 94.5 mL/kg, 158 mL/kg, and 2.26 mL/kg, respectively. Batch water analysis revealed a rapid reaction between constituents in the wash water and chlorine where over a 90 min observation period, 50% of chlorine demand occurred within first 5 min, underscoring the challenge for any water treatment process to reduce chlorine demand once vegetables are deposited into washing systems. Moreover, the results also showed sustained chlorine demand over 90-min periods, indicating an accumulative effect on chlorine consumption with continuous organic input. Additionally, HPLC-SEC analysis showed that the constituents contributing to chlorine demand are predominantly dissolved small molecules (<3400 Da), which will challenge water reuse treatment approaches. This study provides quantitative information of chlorine demand origins and chlorine decay kinetics in wash water and provides baseline data critical for integrating water reuse in the fresh produce processing industry. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Weng, ShihChi; Jacangelo, Joseph G.; Schwab, Kellogg J.] Johns Hopkins Univ, JHU MWH Alliance, Baltimore, MD 21205 USA. [Luo, Yaguang; Li, Jie; Zhou, Bin] USDA ARS, Food Qual & Environm Microbial & Food Safety Labs, Beltsville, MD 20705 USA. [Li, Jie] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China. [Jacangelo, Joseph G.] MWH Global Inc, Pasadena, CA 91101 USA. [Jacangelo, Joseph G.; Schwab, Kellogg J.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA. RP Schwab, KJ (reprint author), Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, 615 N Wolfe St,Room E6620, Baltimore, MD 21205 USA. EM kschwab1@jhu.edu FU USDA-NIFA Specialty Crop Research Initiative Grant [2010-51181-21230]; MWH/JHU Alliance; Hopkins Water Institute; Osprey Foundation of Maryland FX This work is supported by USDA-NIFA Specialty Crop Research Initiative Grant Award No. 2010-51181-21230, the MWH/JHU Alliance, The Hopkins Water Institute, and the Osprey Foundation of Maryland. We would like to thank Jason Bishai for technical laboratory support. NR 40 TC 3 Z9 3 U1 7 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD FEB PY 2016 VL 60 BP 543 EP 551 DI 10.1016/j.foodcont.2015.08.031 PG 9 WC Food Science & Technology SC Food Science & Technology GA CW3HP UT WOS:000364882900073 ER PT J AU Zhang, XY Wen, K Wang, ZH Jiang, HY Beier, RC Shen, JZ AF Zhang, Xiya Wen, Kai Wang, Zhanhui Jiang, Haiyang Beier, Ross C. Shen, Jianzhong TI An ultra-sensitive monoclonal antibody-based fluorescent microsphere immunochromatographic test strip assay for detecting aflatoxin M-1 in milk SO FOOD CONTROL LA English DT Article DE Aflatoxin M-1; Immunochromatographic test strip; Fluorescent microsphere; Monoclonal antibody ID LINKED-IMMUNOSORBENT-ASSAY; CHEMILUMINESCENT ENZYME-IMMUNOASSAY; PERFORMANCE LIQUID-CHROMATOGRAPHY; RAPID DETECTION; ANIMAL-TISSUES; SWINE URINE; M1; VALIDATION; ELISA; GOLD AB A rapid lateral flow fluorescent microsphere immunochromatographic test strip (FM-ICTS) assay has been developed for the detection of aflatoxin M-1 (AFM(1)) residues in milk. For this purpose, an ultra-sensitive anti-AFM(1) monoclonal antibody (MAb) 1D3 was prepared and identified. The IC50 value of the MAb 1D3 for AFM(1) was 23 ng/L, and the cross-reactivity (CR) of the MAb with aflatoxin M-2, aflatoxin B-1, aflatoxin B-2, aflatoxin G(1) and aflatoxin G(2) was 18%, 9%, <0.15%, 43% and <03%, respectively. The MAb was covalently conjugated with carboxylate-modified FMs, and the FMs were used as the label in a competitive immunochromatography assay. Using a moderate hapten-to-protein coupling ratio (2) in the coating antigen resulted in improved immunoassay sensitivity. Under optimal conditions, the IC50 value of the assay was 36.3 ng/L with the limit of detection (LOD) of 4.4 ng/L in milk samples using a fluorescence reader, and the recoveries of AFM(1) in spiked milk samples ranged from 76.6 to 110.8% with coefficient of variation (CV) of 4-14.7%. The whole procedure could be completed within 30 min. The results demonstrated that the FM-ICTS assay is easy to conduct, rapid, highly sensitive and specific for the detection of AFM(1) residues in milk. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Zhang, Xiya; Wen, Kai; Wang, Zhanhui; Jiang, Haiyang; Shen, Jianzhong] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China. [Zhang, Xiya; Wen, Kai; Wang, Zhanhui; Jiang, Haiyang; Shen, Jianzhong] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China. [Wen, Kai; Shen, Jianzhong] Beijing Key Lab Detect Technol Anim Derived Food, Beijing 100193, Peoples R China. [Wang, Zhanhui] Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China. [Jiang, Haiyang; Shen, Jianzhong] Natl Reference Lab Vet Drug Residues, Beijing 100193, Peoples R China. [Beier, Ross C.] USDA ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Shen, JZ (reprint author), China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China. EM sjz@cau.edu.cn OI Beier, Ross/0000-0002-3445-1008 FU International Science & Technology Cooperation Program of China [2009DFA32330]; Special Fund for Agro-scientific Research in the Public Interest [201203040] FX This work was supported by the International Science & Technology Cooperation Program of China (2009DFA32330) and the Special Fund for Agro-scientific Research in the Public Interest (No.201203040). NR 35 TC 4 Z9 4 U1 26 U2 106 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD FEB PY 2016 VL 60 BP 588 EP 595 DI 10.1016/j.foodcont.2015.08.040 PG 8 WC Food Science & Technology SC Food Science & Technology GA CW3HP UT WOS:000364882900079 ER PT J AU Yadav, MP Hicks, KB Johnston, DB Hotchkiss, AT Chau, HK Hanah, K AF Yadav, Madhav P. Hicks, Kevin B. Johnston, David B. Hotchkiss, Arland T., Jr. Chau, Hoa K. Hanah, Kyle TI Production of bio-based fiber gums from the waste streams resulting from the commercial processing of corn bran and oat hulls SO FOOD HYDROCOLLOIDS LA English DT Article; Proceedings Paper CT 12th International Hydrocolloids Conference CY MAY 05-09, 2014 CL Taipei, TAIWAN DE Waste stream; Bio-based fiber gum; Arabinoxylan; Ultrafiltration; Emulsions; Antioxidant ID EMULSIFYING PROPERTIES; MAIZE BRAN; HEMICELLULOSE; EXTRACTION; HYDROLYSIS; IDENTIFICATION AB The U.S. food and non-food industries would benefit from domestically produced crude, semi-pure and pure bio-based fiber gums from corn bran and oat hulls. Corn bran and oat hulls are processed to produce commercial cellulose enriched fiber gels, which generate an alkaline waste stream containing many valuable and functional components, including bio-based fiber gums. In this study, the corn bran and oat hulls processing waste streams were concentrated and drum or spray dried to produce solid products, called crude bio-based fiber gum (crude BFG). Alternatively semi-pure products (semi-pure BFG) were prepared by ethanol precipitation of the concentrated waste stream. Semi-pure product was also produced by purification of the original unconcentrated waste stream using 10, 50 and 100 kDa MWCO ultrafiltration membranes. A highly pure BFG was prepared from the unconcentrated waste stream by first adjusting its pH to 4, removing the precipitated material and precipitating the resulting supernatant with two times its volume with ethanol. All four kinds of BFGs contain arabinoxylan but with different levels of purity. The arabinoxylans have a typical structure with a slight variation in Ara/Xyl ratio showing some differences in their branching. There is a big variation in their weight average molar mass (Mw) (33-386 kDa) due to variation in the processing conditions of the waste streams. Arabinoxylans are good emulsifiers for oil-in-water emulsion systems and binding agents for petroleum coke pellets and briquettes. They are also useful antioxidants and soluble dietary fibers with antioxidant activities showing a great potential for their applications in both food and non-food industries. Published by Elsevier Ltd. C1 [Yadav, Madhav P.; Hicks, Kevin B.; Johnston, David B.; Hotchkiss, Arland T., Jr.; Chau, Hoa K.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Hanah, Kyle] Z Trim Holding Inc, Mundelein, IL 60060 USA. RP Yadav, MP (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM madhav.yadav@ars.usda.gov NR 25 TC 3 Z9 3 U1 9 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD FEB PY 2016 VL 53 BP 125 EP 133 DI 10.1016/j.foodhyd.2015.02.017 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CW3YR UT WOS:000364928800017 ER PT J AU Qiu, S Yadav, MP Liu, Y Chen, H Tatsumi, E Yin, LJ AF Qiu, Shuang Yadav, Madhav P. Liu, Yan Chen, Hao Tatsumi, Eizo Yin, Lijun TI Effects of corn fiber gum with different molecular weights on the gelatinization behaviors of corn and wheat starch SO FOOD HYDROCOLLOIDS LA English DT Article; Proceedings Paper CT 12th International Hydrocolloids Conference CY MAY 05-09, 2014 CL Taipei, TAIWAN DE Corn starch; Wheat starch; Gelatinization; Corn fiber gum; Molecular weight ID KONJAC-GLUCOMANNAN; MAIZE STARCH; GUAR GUM; RETROGRADATION BEHAVIOR; RHEOLOGICAL PROPERTIES; RICE STARCH; GELS; HYDROCOLLOIDS; MIXTURES; OLIGOSACCHARIDES AB Corn fiber gum (CFG) is a novel arabinoxylan hydrocolloid and recent research indicates that it has a considerable potential in food processing. In this study, composite systems consisting of starch and CFG with different molecular characteristics have been evaluated. The pasting and thermal properties, microstructure and leached amylose of corn and wheat starch have been studied. When compared with the starch suspensions alone, CFG addition led to lowering of peak viscosity, breakdown and final viscosity of corn starch. It also led to lowering of peak viscosity and final viscosity, but increase of breakdown of wheat starch The changes in rapid viscosity analyzer (RVA) characteristics were less pronounced as the M-w of CFG was increased. The amount of leached amylose of corn/wheat starch gels decreased as the M-w of CFG increased. The thermal characteristics of corn starch/CFG mixtures varied insignificantly as determined in differential scanning calorimetry (DSC) heating process. Confocal laser scanning microscopy (CLSM) confirmed changes in gel microstructure as starch components tended to be inhibited from leaching out of the granules. CFG addition also led to a well-defined morphology of wheat starch granules after gelatinization, suggesting a leaching-inhibition effect of CFG on the granule structure. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Qiu, Shuang; Liu, Yan; Chen, Hao; Yin, Lijun] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China. [Yadav, Madhav P.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Tatsumi, Eizo] Minist Agr Forestry & Fisheries, Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan. [Yin, Lijun] Henan Univ Technol, Zhengzhou 450052, Henan, Peoples R China. RP Yin, LJ (reprint author), POB 40,17 Qinghuadonglu, Beijing 100083, Peoples R China. EM ljyin@cau.edu.cn FU National Science Foundation of China [31371719]; National Key technologies RD Project [2012BAD37B05, 2014BAD04B06]; special fund for agro-scientific research in the public interest [201303079] FX This research was carried out with financial support from the National Science Foundation of China (No. 31371719), National Key technologies R&D Project (No. 2012BAD37B05; 2014BAD04B06) and special fund for agro-scientific research in the public interest (No. 201303079). The authors are pleased to acknowledge Dr. Madhuvanti Kale (ERRC, ARS, USDA) for editing and doing critical review of the manuscript and Stefanie Simon (ERRC, ARS, USDA) for her technical assistance. NR 35 TC 1 Z9 2 U1 22 U2 84 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD FEB PY 2016 VL 53 BP 180 EP 186 DI 10.1016/j.foodhyd.2015.01.034 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CW3YR UT WOS:000364928800023 ER PT J AU Mirzabaev, A Ahmed, M Werner, J Pender, J Louhaichi, M AF Mirzabaev, Alisher Ahmed, Mohamed Werner, Jutta Pender, John Louhaichi, Mounir TI Rangelands of Central Asia: challenges and opportunities SO JOURNAL OF ARID LAND LA English DT Article DE rangeland degradation; flock mobility; overgrazing; sustainable rangeland management; drylands; land tenure ID TURKMENISTAN; DEGRADATION; MANAGEMENT; VEGETATION; LIVESTOCK; KARAKUM; DESERT; SCALE AB Rangelands of Central Asia (referring to Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan and Uzbekistan in this study), the largest contiguous area of grazed land in the world, serve as an important source of livelihood for pastoral and agro-pastoral communities in this region. They also play an important role in absorbing CO2 as a global carbon sink. However, unsustainable management of rangelands has led to their degradation hugely by downgrading their potential agro-ecological, environmental and socio-economical roles. This paper reviewed the rangeland degradation in Central Asia, a topic which so far has received only scant coverage in the international scientific literature. It also provided examples of successful experiences and outlined possible options that land managers can adopt to enhance the sustainable management of these vast degraded rangelands. The experiences and lessons described in this paper may also be relevant for other degraded rangeland areas, especially in the developing countries. The causes of rangeland degradation within the Central Asian region are numerous, complex and inter-related. Therefore, while addressing the factors associated with improper rangeland management may shed some light on the causes of rangeland degradation, the scope of this paper would not be all-encompassing for the major causes of degradation. There is a need to develop and widely apply the viable and locally accepted and adapted packages of technical, institutional and policy options for sustainable rangeland management. Incentivizing the collective action of small-scale pastoralists who group together to facilitate access to remote pastures can reduce the degree of overgrazing within community pastures, such as those near the settlements. We also found that migratory grazing through pooling of resources among small-scale pastoralists can increase household income. After their independence, most Central Asian countries adopted various rangeland tenure arrangements. However, the building of enhanced capacities of pasture management and effective local rangeland governance structures can increase the likelihood, which will be sustainable and equitable. Finally, this paper presented several promising technical options, aiming at reversing the trend of rangeland degradation in Central Asia. C1 [Mirzabaev, Alisher] Univ Bonn, Res Dev Ctr, D-53113 Bonn, Germany. [Ahmed, Mohamed] Food & Agr Org United Nat, Agr Dev Econ Div, Monitoring African Food & Agr Policies, I-00153 Rome, Italy. [Werner, Jutta; Louhaichi, Mounir] Int Ctr Agr Res Dry Areas, Div & Sustainable Intensificat Prod Syst Program, Amman 11195, Jordan. [Pender, John] ERS, USDA, Farm & Rural Household Well Being Branch, Washington, DC 20036 USA. RP Louhaichi, M (reprint author), Int Ctr Agr Res Dry Areas, Div & Sustainable Intensificat Prod Syst Program, Amman 11195, Jordan. EM M.Louhaichi@cgiar.org FU Asian Development Bank; International Center for Agricultural Research in the Dry Areas, Russian Scientific Fund [14-38-00023]; CGIAR Research Program on Dryland Systems FX We would like to thank the Asian Development Bank, the International Center for Agricultural Research in the Dry Areas, Russian Scientific Fund (14-38-00023) and the CGIAR Research Program on Dryland Systems for their support and funding. Special thanks also go to Dr. Kathryn CLIFTON and Dr. Steven PETERSEN for editing and formatting this manuscript. NR 79 TC 1 Z9 2 U1 6 U2 34 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1674-6767 EI 2194-7783 J9 J ARID LAND JI J. Arid Land PD FEB PY 2016 VL 8 IS 1 BP 93 EP 108 DI 10.1007/s40333-015-0057-5 PG 16 WC Environmental Sciences SC Environmental Sciences & Ecology GA CW5JQ UT WOS:000365032100008 ER PT J AU Liu, ZJ Mi, BB Jiang, ZH Fei, BH Cai, ZY Liu, X AF Liu, Zhijia Mi, Bingbing Jiang, Zehui Fei, Benhua Cai, Zhiyong Liu, Xing'e TI Improved bulk density of bamboo pellets as biomass for energy production SO RENEWABLE ENERGY LA English DT Article DE Biomass; Bio-energy; Moso bamboo; Pine; Pellet AB To the best of our knowledge, there is the lack of sufficient information concerning bamboo pellets. In the preliminary research, bamboo pellets showed a low bulk density which could not meet requirement of Pellet Fuels Institute Standard Specification for Residential/Commercial Densified (PFI). To improve its bulk density, pellets were manufactured using mixtures of bamboo and pine particles and the properties were investigated. It was found that adding pine particles to bamboo particles was an effective way to improve bulk density of bamboo pellets. When adding 40% pine particles to bamboo particles, bulk density of pellets increased from 0.54 g/cm(3) to 0.60 g/cm(3), meeting grade requirement of PF1 utility. Furthermore, length, diameter and inorganic ash of pellets were also improved. Fine contents of pellets decreased from premium grade to utility grade according to PFI standard. Net calorific value also slightly decreased but it could meet the requirement of DIN 51731 (>17,500 J/g). The effect of this interaction on bulk density, inorganic ash, Net calorific value, combustion rate and heat release rate were significant. The results from this research will be very helpful to develop bamboo pellets and provide guidelines for further research. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Liu, Zhijia; Mi, Bingbing; Jiang, Zehui; Fei, Benhua; Liu, Xing'e] Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. [Cai, Zhiyong] USDA Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. RP Jiang, ZH (reprint author), Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. EM liuzj@icbr.ac.cn FU Basic Scientific Research Funds of International Centre for Bamboo and Rattan [1632012002]; 12th Five Years Plan-Study on manufacturing technology of functional bamboo (rattan)-based materials [2012BAD54G01] FX This research was financially supported by 'Basic Scientific Research Funds of International Centre for Bamboo and Rattan' (Grant No. 1632012002) and '12th Five Years Plan-Study on manufacturing technology of functional bamboo (rattan)-based materials' (Grant No. 2012BAD54G01). NR 19 TC 2 Z9 2 U1 3 U2 34 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD FEB PY 2016 VL 86 BP 1 EP 7 DI 10.1016/j.renene.2015.08.011 PG 7 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA CV4PF UT WOS:000364248300001 ER PT J AU Kamil, A Smith, DE Blumberg, JB Astete, C Sabliov, C Chen, CYO AF Kamil, Alison Smith, Donald E. Blumberg, Jeffrey B. Astete, Carlos Sabliov, Cristina Chen, C. -Y. Oliver TI Bioavailability and biodistribution of nanodelivered lutein SO FOOD CHEMISTRY LA English DT Article DE Bioavailability; Caco-2 cells; Lutein (PubChem CID: 5281243); Nanoparticles; Poly(lactic-co-glycolic acid) (PubChem CID: 23111554); Rats ID LOADED PLGA NANOPARTICLES; DRUG-DELIVERY SYSTEMS; IN-VITRO; CAROTENOID BIOAVAILABILITY; MOLECULAR-WEIGHT; CELLULAR UPTAKE; CACO-2 CELLS; TRANSPORT; RATS; VIVO AB The aim of the study was to evaluate the ability of poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP) to enhance lutein bioavailability. The bioavailability of free lutein and PLGA-NP lutein in rats was assessed by determining plasma pharmacokinetics and deposition in selected tissues. Lutein uptake and secretion was also assessed in Caco-2 cells. Compared to free lutein, PLGA-NP increased the maximal plasma concentration (Cm) and area under the time-concentration curve in rats by 54.5- and 77.6-fold, respectively, while promoting tissue accumulation in the mesenteric fat and spleen. In comparison with micellized lutein, PLGA-NP lutein improved the C-max in rat plasma by 15.6-fold and in selected tissues by >3.8-fold. In contrast, PLGA-NP lutein had a lower uptake and secretion of lutein in Caco-2 cells by 10.0- and 50.5-fold, respectively, compared to micellized lutein. In conclusion, delivery of lutein with polymeric NP may be an approach to improve the bioavailability of lutein in vivo. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Kamil, Alison; Smith, Donald E.; Blumberg, Jeffrey B.; Chen, C. -Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. [Astete, Carlos; Sabliov, Cristina] Louisiana State Univ, Dept Biol & Agr Engn, Baton Rouge, LA 70803 USA. RP Chen, CYO (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, 711 Washington St, Boston, MA 02111 USA. EM Oliver.Chen@tufts.edu FU US Department of Agriculture (USDA) National Institute of Food and Agriculture [2011-67-21-30375]; USDA/Agricultural Research Service [1950-5100-087] FX This work was supported by US Department of Agriculture (USDA) National Institute of Food and Agriculture (Award 2011-67-21-30375) and the USDA/Agricultural Research Service (Cooperative Agreement No. 1950-5100-087). AK conducted the experiments, performed statistical analysis, and prepared the manuscript. DES contributed to study design, sample collection, and data interpretation. SC and CA acquired the unmodified lutein and produced the nanoparticle lutein. JBB and CYOC contributed to the study design, data interpretation, and manuscript preparation. All authors report no conflict of interest. The content of this publication do not necessarily reflect the views or policies of the USDA nor does mention of trade names, commercial products or organizations imply endorsement by the US government. NR 40 TC 0 Z9 1 U1 8 U2 111 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD FEB 1 PY 2016 VL 192 BP 915 EP 923 DI 10.1016/j.foodchem.2015.07.106 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CS7ZC UT WOS:000362304500117 PM 26304429 ER PT J AU Lima, RSN Garcia-Tejero, I Lopes, TS Costa, JM Vaz, M Duran-Zuazo, VH Chaves, M Glenn, DM Campostrini, E AF Lima, R. S. N. Garcia-Tejero, I. Lopes, T. S. Costa, J. M. Vaz, M. Duran-Zuazo, V. H. Chaves, M. Glenn, D. M. Campostrini, E. TI Linking thermal imaging to physiological indicators in Carica papaya L. under different watering regimes SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Papaya; Thermography; Crop-water status monitoring; Deficit irrigation ID CHLOROPHYLL-FLUORESCENCE; DEFICIT IRRIGATION; STOMATAL CLOSURE; PLANT-RESPONSES; USE EFFICIENCY; STRESS; GRAPEVINE; DROUGHT; CROP; ECOPHYSIOLOGY AB Water deficit is the most limiting factor for yield and fruit-quality parameters in papaya crop (Carica papaya L.), deficit-irrigation (DI) strategies offering a feasible alternative to manage limiting water resources. When DI is applied, it is crucial to assess the physiological status of the crop in order to maintain the plant within a threshold value of water stress so as no to affect yield or fruit-quality parameters. The aim of this work was to evaluate the feasibility of thermal imaging in young papaya plants to assess the physiological status of this crop when it is subjected to different DI regimes, studying the relationships between the changes in leaf temperature (T-leaf) and in the major physiological parameters (i.e., stomatal conductance to water vapor, g(s); transpiration, E; and net photosynthesis, A(n)). The trial was conducted in a greenhouse from March to April of 2012. Plants were grown in pots and subjected to four irrigation treatments: (1) a full irrigation treatment (control), maintained at field capacity; (2) a partial root-zone drying treatment, irrigated with 50% of the total water applied to control to only one side of roots, alternating the sides every 7 days; (3) a regulated deficit irrigation (50% of the control, applied to both sides of plant); (4) and a non-irrigated treatment, in which irrigation was withheld from both sides of the split root for 14 days, followed by full irrigation until the end of the study. Significant relationships were found between T-leaf and major physiological variables such as g(s), E and A(n). Additionally, significant relationships were found between the difference of leaf-to-air temperature (Delta Tleaf-air) and gas-exchange measurements, which were used to establish the optimum range of Delta Tleaf-air as a preliminary step to the crop-water monitoring and irrigation scheduling in papaya, using thermal imaging as the main source of information. According to the results, we conclude that thermal imaging is a promising technique to monitor the physiological status of papaya during drought conditions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lima, R. S. N.; Vaz, M.] Univ Evora, ICAAM, Escola Ciencias & Tecnol, Dept Biol, P-7002554 Evora, Portugal. [Lima, R. S. N.] Univ Fed Sergipe, BR-49100000 Sao Cristovao, SE, Brazil. [Garcia-Tejero, I.; Duran-Zuazo, V. H.] Ctr IFAPA Las Torres Tomejil, Seville 41200, Spain. [Campostrini, E.] Univ Estadual Norte Fluminense Darcy Ribeiro UENF, CCTA, LMGV, BR-28013600 Campos Dos Goytacazes, RJ, Brazil. [Costa, J. M.; Chaves, M.] UNL, ITQB, Lab Ecofisiol Mol, P-2780901 Oeiras, Portugal. [Glenn, D. M.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Garcia-Tejero, I (reprint author), Ctr IFAPA Las Torres Tomejil, Ctra Sevilla Cazalla,Km 12,2, Seville 41200, Spain. EM ivanf.garcia@juntadeandalucia.es RI Garcia-Tejero, Ivan Francisco/C-4143-2016; OI Garcia-Tejero, Ivan Francisco/0000-0002-4606-0880; Costa, Joaquim/0000-0001-5888-947X; Campostrini, Eliemar/0000-0002-1329-1084 FU Operational Programme of the European Social Fund (ESF) "Andalucia is moving with Europe" FX The author I. Garcia-Tejero has a contract co-financed by the Operational Programme of the European Social Fund (ESF) 2007-2013 "Andalucia is moving with Europe". NR 58 TC 1 Z9 1 U1 50 U2 65 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JAN 31 PY 2016 VL 164 SI SI BP 148 EP 157 DI 10.1016/j.agwat.2015.07.017 PN 1 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DA8GE UT WOS:000368042300017 ER PT J AU Adhikari, P Ale, S Bordovsky, JP Thorp, KR Modala, NR Rajan, N Barnes, EM AF Adhikari, Pradip Ale, Srinivasulu Bordovsky, James P. Thorp, Kelly R. Modala, Naga R. Rajan, Nithya Barnes, Edward M. TI Simulating future climate change impacts on seed cotton yield in the Texas High Plains using the CSM-CROPGRO-Cotton model SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Climate models; Climate change; Crop model; DSSAT; Irrigation; Seed cotton yield ID CARBON-DIOXIDE; IRRIGATION STRATEGIES; WATER; AGRICULTURE; CO2; EVAPOTRANSPIRATION; SENSITIVITY; MANAGEMENT; AUSTRALIA; RESPONSES AB The Texas High Plains (THP) region contributes to about 25% of the US cotton production. Dwindling groundwater resources in the underlying Ogallala aquifer, future climate variability and frequent occurrences of droughts are major concerns for cotton production in this region. Assessing the impacts of climate change on cotton production enables development and evaluation of irrigation strategies for efficient utilization of groundwater resources in this region. In this study, the CROPGRO-Cotton module within the Cropping System Model (CSM) that is distributed with the Decision Support System for Agrotechnology Transfer (DSSAT) was evaluated for the THP region using measured data from cotton water use efficiency experiments at Halfway over a period of four years (2010-2013). Simulated seed cotton yield matched closely with observed yield during model calibration (average percent error of 0.1) and validation (average percent error of 6.5). The evaluated model was able to accurately simulate seed cotton yield under various irrigation strategies over the four growing seasons. The evaluated CROPGRO-Cotton model was later used to simulate the seed cotton yield under historic (1971-2000) and future (2041-2070) climate scenarios projected by three climate models. On an average, when compared to historic seed cotton yield, simulated future seed cotton yield across the THP decreased within a range of 4-17% when carbon dioxide (CO2) concentration was assumed to be constant at the current level (380 ppm) under three climatic model scenarios. In contrast, when the CO2 concentration was assumed to increase from 493 ppm (in year 2041) to 635 ppm (in year 2070) according to the Intergovernmental Panel on Climate Change (IPCC) A2 emission scenario, the simulated future average seed cotton yield in the THP region increased within a range of 14-29% as compared to historic average yield. When the irrigation amount was reduced by 40% (from 100% to 60%), the average (2041-2070) seed cotton yield decreased by 37% and 39% under the constant and increasing CO2 concentration scenarios, respectively. These results imply that cotton is sensitive to atmospheric CO2 concentrations, and cotton production in the THP could potentially withstand the effects of future climate variability under moderate increases in CO2 levels if irrigation water availability remains at current levels. (C) 2015 Elsevier B.V. All rights reserved. C1 [Adhikari, Pradip; Ale, Srinivasulu] Texas A&M Univ Syst, Texas A&M AgriLife Res, Vernon, TX 76385 USA. [Bordovsky, James P.] Texas A&M Univ Syst, Texas A&M AgriLife Res, Halfway, TX 79072 USA. [Thorp, Kelly R.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 58138 USA. [Modala, Naga R.] IntegraShare Solutioneering Inc, The Woodlands, TX 77380 USA. [Rajan, Nithya] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 76384 USA. [Barnes, Edward M.] Cotton Inc, Agr & Environm Res, Cary, NC 27513 USA. RP Ale, S (reprint author), Texas A&M Univ Syst, Texas A&M AgriLife Res, Vernon, TX 76385 USA. EM sriniale@ag.tamu.edu RI Thorp, Kelly/C-2013-2009; OI Thorp, Kelly/0000-0001-9168-875X; Modala, Naga Raghuveer/0000-0003-0899-2561 FU Cotton Incorporated FX We gratefully acknowledge the funding support provided by the Cotton Incorporated for this study. We thank Dr. Katie Lewis, Assistant Professor, Texas A&M AgriLife Research, Lubbock and Mr. Sumit, Graduate student at Texas Tech for their help in the collection of soil samples. We thank the reviewers for their valuable time and helpful suggestions for improving the manuscript. NR 59 TC 1 Z9 1 U1 5 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JAN 31 PY 2016 VL 164 BP 317 EP 330 DI 10.1016/j.agwat.2015.10.011 PN 2 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DA8GF UT WOS:000368042400011 ER PT J AU Rathinam, T Gadde, U Chapman, HD AF Rathinam, T. Gadde, U. Chapman, H. D. TI Attenuation of a drug-sensitive strain of a turkey protozoan parasite Eimeria meleagrimitis by selection for precocious development SO VETERINARY PARASITOLOGY LA English DT Article DE Eimeria meleagrimitis; Turkey; Pathogenicity; Immunogenicity; Attenuation ID COCCIDIOSIS; MONENSIN; POULT AB An attenuated line of Eimeria meleagrimitis was established by repeated propagation of the parasite in 9-day old turkey poults and subsequent selection for precocious development. Following 20 passages, the prepatent period decreased from 120 to 104 h. A series of experiments were conducted to evaluate the pathogenicity, immunogenicity and fecundity of the newly selected line. Judged by body weight gain, feed consumption and feed efficiency following infection, the attenuated line had appreciably reduced pathogenicity. Immunogenicity of the attenuated line was examined by infecting poults successively with incremental doses of 10(2), 10(3) and 10(4) oocysts at 0, 7, and 14 days of age respectively. No oocysts were detected following challenge with 5 x 10(2) oocysts, indicating that the attenuated line had retained immunogenicity. Fecundity was assessed by infecting two-week old birds with 5 x 10(2) oocysts of either parent or attenuated line. Oocyst production from 96 to 240 h post-infection showed that the patent period of the attenuated line commenced earlier and was of shorter duration than the parent line. (C) 2015 Elsevier B.V. All rights reserved. C1 [Rathinam, T.; Gadde, U.; Chapman, H. D.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Gadde, U.] USDA ARS, ABBL, Beltsville, MD USA. RP Rathinam, T (reprint author), Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. EM thilak.rathinam@outlook.com NR 10 TC 0 Z9 0 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JAN 30 PY 2016 VL 216 BP 1 EP 3 DI 10.1016/j.vetpar.2015.11.014 PG 3 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DC8KZ UT WOS:000369469800001 PM 26801586 ER PT J AU Fiuza, VRD Lopes, CWG de Oliveira, FCR Fayer, R Santin, M AF da Silva Fiuza, Vagner Ricardo Gomes Lopes, Carlos Wilson Rodrigues de Oliveira, Francisco Carlos Fayer, Ronald Santin, Monica TI New findings of Enterocytozoon bieneusi in beef and dairy cattle in Brazil SO VETERINARY PARASITOLOGY LA English DT Article DE Brazil; Cattle; Enterocytozoon bieneusi; Genotypes ID EASTERN UNITED-STATES; HUMAN-PATHOGENIC MICROSPORIDIA; MOLECULAR CHARACTERIZATION; CRYPTOSPORIDIUM SPP.; INTESTINAL MICROSPORIDIOSIS; CYCLOSPORA-CAYETANENSIS; GIARDIA-DUODENALIS; 1ST DETECTION; WASTE-WATER; PREVALENCE AB Microsporidia are widely recognized as important human pathogens with Enterocytozoon bieneusi as the most common species infecting humans and animals, including cattle. Although Brazil has the second largest cattle herd in the world and it is the largest exporter of beef there are no data on the presence or impact of E. bieneusi on this important population. To fill this knowledge gap, fecal specimens were collected from 452 cattle from pre-weaned calves to adult cattle in the state of Rio de Janeiro. Host factors including age, gender, dairy/beef, body composition, and fecal consistency were included in the study. Using molecular methods, E. bieneusi was found in 79/452 (17.5%) fecal specimens. This represents the first report of this parasite in Brazilian cattle. A significantly higher prevalence was found in calves less than 2 months of age (27.6%) and those 3-8 months of age (28.8%) versus heifers (14.1%) and adults (1.4%) (P < 0.05). Dairy cattle (26.2%) had a higher prevalence than beef cattle (9.7%) (P < 0.001). No correlation was found between infection and gender, body composition, and fecal consistency. Molecular characterization of the internal transcribed spacer (ITS) revealed 12 genotypes; five previously reported in cattle (BEB4, BEB8, D, EbpA and I), and seven novel genotypes (BEB11-BEB17). A phylogenetic analysis showed that 6 genotypes (D, EbpA, BEB12, BEB13, BEB15, and BEB16) identified in 18 animals clustered within the designated zoonotic Group 1 while the other 6 genotypes (I, BEB4, BEB8, BEB11, BEB14, BEB17) identified in 61 animals clustered within Group 2. The identification of genotypes in Brazilian cattle that have previously been reported in humans highlights the potential risk of zoonotic transmission and suggests that the role of cattle in transmission of human infections requires further study. Published by Elsevier B.V. C1 [da Silva Fiuza, Vagner Ricardo; Fayer, Ronald; Santin, Monica] ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. [da Silva Fiuza, Vagner Ricardo; Gomes Lopes, Carlos Wilson] UFRRJ, Inst Vet, Dept Parasitol Anim, BR 465 Km 7, BR-23897970 Seropedica, RJ, Brazil. [Rodrigues de Oliveira, Francisco Carlos] Univ Estadual Norte Fluminense, BR-28013602 Rio De Janeiro, Brazil. RP Santin, M (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM monica.santin-duran@ars.usda.gov FU Ministry of Education of Brazil [BEX 2705-13-3] FX This study was supported by Ministry of Education of Brazil, process no BEX 2705-13-3. NR 48 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JAN 30 PY 2016 VL 216 BP 46 EP 51 DI 10.1016/j.vetpar.2015.12.008 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DC8KZ UT WOS:000369469800009 ER PT J AU Trupkiewicz, JG Calero-Bernal, R Verma, SK Mowery, J Davison, S Habecker, P Georoff, TA Ialeggio, DM Dubey, JP AF Trupkiewicz, J. G. Calero-Bernal, R. Verma, S. K. Mowery, J. Davison, S. Habecker, P. Georoff, T. A. Ialeggio, D. M. Dubey, J. P. TI Acute, fatal Sarcocystis calchasi-associated hepatitis in Roller pigeons (Columba livia f. dom.) at Philadelphia Zoo SO VETERINARY PARASITOLOGY LA English DT Article DE Sarcocystis calchasi; Roller pigeons (Columba livia); Schizonts; Hepatitis ID PROTOZOAL ENCEPHALITIS; FALCATULA INFECTIONS; NORTH-AMERICA; NEURONA; DISEASE; DOMESTICA AB Four Roller pigeons (Columba livia f. dom.) at the Philadelphia Zoo died suddenly. Necropsy examination revealed macroscopic hepatitis. Microscopically, the predominant lesions were in liver, characterized with necrosis and mixed cell inflammatory response. Sarcocystis calchasi-like schizonts and free merozoites were identified in liver. Transmission electron microscopy confirmed that schizonts were in hepatocytes. A few schizonts were in spleen. PCR using S. calchasi-specific primers confirmed the diagnosis. Neither lesions nor protozoa were found in brain and muscles. This is the first report of acute visceral S. calchasi-associated sarcocystosis in naturally infected avian hosts. Published by Elsevier B.V. C1 [Trupkiewicz, J. G.; Georoff, T. A.; Ialeggio, D. M.] Philadelphia Zoo, 3400 W Girard Ave, Philadelphia, PA 19104 USA. [Calero-Bernal, R.; Verma, S. K.; Dubey, J. P.] ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Bldg 1001, Beltsville, MD 20705 USA. [Mowery, J.] ARS, USDA, Electron & Confocal Microscopy Unit, Beltsville Agr Res Ctr, Bldg 12, Beltsville, MD 20705 USA. [Davison, S.; Habecker, P.] Univ Penn, Sch Vet Med, New Bolton Ctr, Penn Anim Diagnost Lab Syst, 382 West St Rd, Kennett Sq, PA 19348 USA. RP Dubey, JP (reprint author), ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 26 TC 3 Z9 3 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JAN 30 PY 2016 VL 216 BP 52 EP 58 DI 10.1016/j.vetpar.2015.11.008 PG 7 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DC8KZ UT WOS:000369469800010 PM 26801595 ER PT J AU Raithore, S Dea, S McCollum, G Manthey, JA Bai, JH Leclair, C Hijaz, F Narciso, JA Baldwin, EA Plotto, A AF Raithore, Smita Dea, Sharon McCollum, Greg Manthey, John A. Bai, Jinhe Leclair, Clotilde Hijaz, Faraj Narciso, Jan A. Baldwin, Elizabeth A. Plotto, Anne TI Development of delayed bitterness and effect of harvest date in stored juice from two complex citrus hybrids SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE mandarin; tangerine; orange juice; 'Ambersweet'; sensory; limonin; nomilin ID ORANGE JUICE; TASTE RECEPTORS; RING-LACTONE; LIMONIN; QUALITY; EXTRACTION AB BACKGROUNDMandarins and mandarin hybrids have excellent flavor and color attributes, making them good candidates for consumption as fresh fruit. When processed into juice, however, they are less palatable, as they develop delayed bitterness when stored for a period of time. In this study the kinetics of delayed bitterness in two citrus mandarin hybrid siblings, Ambersweet' and USDA 1-105-106, was explored by sensory and instrumental analyses. In addition to the bitter limonoids, other quality factors (i.e. sugars, acids, pH, soluble solids content (SSC), titratable acidity (TA) and the ratio SSC/TA) were also measured. RESULTSThe two citrus hybrid siblings had different chemical profiles, which were perceived by taste panels. USDA 1-105-106 developed delayed bitterness when the juice was stored for more than 4h, similar to juice from Navel' oranges, but Ambersweet' did not. Bitterness in Ambersweet' was more affected by harvest maturity, as juice from earlier harvest had lower SSC but higher TA and bitter limonoids. CONCLUSIONSince juice of USDA 1-105-106 shows delayed bitterness when stored for more than 4h, this cultivar is not suitable for juice processing. Our finding that siblings can differ in chemical and sensory properties emphasize the importance of post-processing storage studies before releasing cultivars for juice. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Raithore, Smita; Dea, Sharon; McCollum, Greg; Manthey, John A.; Bai, Jinhe; Narciso, Jan A.; Baldwin, Elizabeth A.; Plotto, Anne] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Leclair, Clotilde] Ecole Natl Super Agron Toulouse, Toulouse, France. [Hijaz, Faraj] Univ Florida, Ctr Citrus Res & Educ, Lake Alfred, FL USA. RP Plotto, A (reprint author), USDA ARS, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM anne.plotto@ars.usda.gov FU BARD [IS-4368-10]; United States-Israel Binational Agricultural Research and Development Fund FX This research was partially funded by Research Grant No. IS-4368-10 from BARD, the United States-Israel Binational Agricultural Research and Development Fund. NR 23 TC 1 Z9 1 U1 4 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD JAN 30 PY 2016 VL 96 IS 2 BP 422 EP 429 DI 10.1002/jsfa.7105 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CZ6PL UT WOS:000367223100008 PM 25615579 ER PT J AU Averett, JP McCune, B Parks, CG Naylor, BJ DelCurto, T Mata-Gonzalez, R AF Averett, Joshua P. McCune, Bruce Parks, Catherine G. Naylor, Bridgett J. DelCurto, Tim Mata-Gonzalez, Ricardo TI Non-Native Plant Invasion along Elevation and Canopy Closure Gradients in a Middle Rocky Mountain Ecosystem SO PLOS ONE LA English DT Article ID WESTERN NORTH-AMERICA; LAND-USE; PATTERNS; RESTORATION; LANDSCAPE; FORESTS; CLIMATE; MANAGEMENT; ROADS AB Mountain environments are currently among the ecosystems least invaded by non-native species; however, mountains are increasingly under threat of non-native plant invasion. The slow pace of exotic plant invasions in mountain ecosystems is likely due to a combination of low anthropogenic disturbances, low propagule supply, and extreme/steep environmental gradients. The importance of any one of these factors is debated and likely ecosystem dependent. We evaluated the importance of various correlates of plant invasions in the Wallowa Mountain Range of northeastern Oregon and explored whether non-native species distributions differed from native species along an elevation gradient. Vascular plant communities were sampled in summer 2012 along three mountain roads. Transects (n = 20) were evenly stratified by elevation (similar to 70 m intervals) along each road. Vascular plant species abundances and environmental parameters were measured. We used indicator species analysis to identify habitat affinities for non-native species. Plots were ordinated in species space, joint plots and non-parametric multiplicative regression were used to relate species and community variation to environmental variables. Non-native species richness decreased continuously with increasing elevation. In contrast, native species richness displayed a unimodal distribution with maximum richness occurring at mid-elevations. Species composition was strongly related to elevation and canopy openness. Overlays of trait and environmental factors onto non-metric multidimensional ordinations identified the montane-subalpine community transition and over-story canopy closure exceeding 60% as potential barriers to non-native species establishment. Unlike native species, non-native species showed little evidence for high-elevation or closed-canopy specialization. These data suggest that non-native plants currently found in the Wallowa Mountains are dependent on open canopies and disturbance for establishment in low and mid elevations. Current management objectives including restoration to more open canopies in dry Rocky Mountain forests, may increase immigration pressure of non-native plants from lower elevations into the montane and subalpine zones. C1 [Averett, Joshua P.; DelCurto, Tim] Oregon State Univ, Eastern Oregon Agr & Nat Resource Program, La Grande, OR USA. [McCune, Bruce] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Parks, Catherine G.; Naylor, Bridgett J.] USDA Forest Serv, Pacific Northwest Res Stn, La Grande, OR USA. [Mata-Gonzalez, Ricardo] Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. RP Averett, JP (reprint author), Oregon State Univ, Eastern Oregon Agr & Nat Resource Program, La Grande, OR USA. EM averettj@onid.oregonstate.edu FU U.S.D.A. Forest Service Pacific Northwest Research Station [11-JV-11261967-064]; Oregon State University FX This project was funded by U.S.D.A. Forest Service Pacific Northwest Research Station, URL: http://www.fs.fed.us/pnw/, Contract (Joint Ventura Agreement with Oregon State University): 11-JV-11261967-064. The funders had no role in the study design, data collection, and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 0 Z9 0 U1 3 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 29 PY 2016 VL 11 IS 1 AR e0147826 DI 10.1371/journal.pone.0147826 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9GH UT WOS:000369528600063 PM 26824750 ER PT J AU DebRoy, C Fratamico, PM Yan, XH Baranzoni, GM Liu, YH Needleman, DS Tebbs, R O'Connell, CD Allred, A Swimley, M Mwangi, M Kapur, V Garay, JAR Roberts, EL Katani, R AF DebRoy, Chitrita Fratamico, Pina M. Yan, Xianghe Baranzoni, GianMarco Liu, Yanhong Needleman, David S. Tebbs, Robert O'Connell, Catherine D. Allred, Adam Swimley, Michelle Mwangi, Michael Kapur, Vivek Garay, Juan A. Raygoza Roberts, Elisabeth L. Katani, Robab TI Comparison of O-Antigen Gene Clusters of All O-Serogroups of Escherichia coli and Proposal for Adopting a New Nomenclature for O-Typing SO PLOS ONE LA English DT Article ID POLYMERASE-CHAIN-REACTION; ABC TRANSPORTER; DNA MICROARRAY; IDENTIFICATION; SEQUENCE; STRAINS; O145; BIOSYNTHESIS; DIVERSITY; PATHWAYS AB Escherichia coli strains are classified based on O-antigens that are components of the lipopolysaccharide (LPS) in the cell envelope. O-antigens are important virulence factors, targets of both the innate and adaptive immune system, and play a role in host-pathogen interactions. Because they are highly immunogenic and display antigenic specificity unique for each strain, O-antigens are the biomarkers for designating O-types. Immunologically, 185 O-serogroups and 11 OX-groups exist for classification. Conventional serotyping for O-typing entails agglutination reactions between the O-antigen and antisera generated against each O-group. The procedure is labor intensive, not always accurate, and exhibits equivocal results. In this report, we present the sequences of 71 O-antigen gene clusters (O-AGC) and a comparison of all 196 O- and OX-groups. Many of the designated O-types, applied for classification over several decades, exhibited similar nucleotide sequences of the O-AGCs and cross-reacted serologically. Some O-AGCs carried insertion sequences and others had only a few nucleotide differences between them. Thus, based on these findings, it is proposed that several of the E. coli O-groups may be merged. Knowledge of the O-AGC sequences facilitates the development of molecular diagnostic platforms that are rapid, accurate, and reliable that can replace conventional serotyping. Additionally, with the scientific knowledge presented, new frontiers in the discovery of biomarkers, understanding the roles of O-antigens in the innate and adaptive immune system and pathogenesis, the development of glycoconjugate vaccines, and other investigations, can be explored. C1 [DebRoy, Chitrita; Mwangi, Michael; Kapur, Vivek; Garay, Juan A. Raygoza; Roberts, Elisabeth L.; Katani, Robab] Penn State Univ, Dept Vet & Biomed Sci, E Coli Reference Ctr, Univ Pk, Pennsauken, NJ USA. [Fratamico, Pina M.; Yan, Xianghe; Baranzoni, GianMarco; Liu, Yanhong; Needleman, David S.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Tebbs, Robert; O'Connell, Catherine D.; Allred, Adam; Swimley, Michelle] Thermo Fisher Sci, Anim Hlth & Food Safety Life Sci Solut, Austin, TX USA. RP DebRoy, C (reprint author), Penn State Univ, Dept Vet & Biomed Sci, E Coli Reference Ctr, Univ Pk, Pennsauken, NJ USA. EM rcd3@psu.edu FU Thermo Fisher Scientific FX Thermo Fisher Scientific partially supported sequencing of the O-antigen gene clusters and purchased some of the reference standard strains. Robert Tebbs and Michelle Swimley contributed on a technical level through generating whole genome sequencing results for some of the strains. Adam Aldred was involved in genome assembly. NR 51 TC 8 Z9 9 U1 4 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 29 PY 2016 VL 11 IS 1 AR e0147434 DI 10.1371/journal.pone.0147434 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9GH UT WOS:000369528600032 PM 26824864 ER PT J AU Tredennick, AT Adler, PB Grace, JB Harpole, WS Borer, ET Seabloom, EW Anderson, TM Bakker, JD Biederman, LA Brown, CS Buckley, YM Chu, CJ Collins, SL Crawley, MJ Fay, PA Firn, J Gruner, DS Hagenah, N Hautier, Y Hector, A Hillebrand, H Kirkman, K Knops, JMH Laungani, R Lind, EM MacDougall, AS McCulley, RL Mitchell, CE Moore, JL Morgan, JW Orrock, JL Peri, PL Prober, SM Risch, AC Schutz, M Speziale, KL Standish, RJ Sullivan, LL Wardle, GM Williams, RJ Yang, LH AF Tredennick, Andrew T. Adler, Peter B. Grace, James B. Harpole, W. Stanley Borer, Elizabeth T. Seabloom, Eric W. Anderson, T. Michael Bakker, Jonathan D. Biederman, Lori A. Brown, Cynthia S. Buckley, Yvonne M. Chu, Chengjin Collins, Scott L. Crawley, Michael J. Fay, Philip A. Firn, Jennifer Gruner, Daniel S. Hagenah, Nicole Hautier, Yann Hector, Andy Hillebrand, Helmut Kirkman, Kevin Knops, Johannes M. H. Laungani, Ramesh Lind, Eric M. MacDougall, Andrew S. McCulley, Rebecca L. Mitchell, Charles E. Moore, Joslin L. Morgan, John W. Orrock, John L. Peri, Pablo L. Prober, Suzanne M. Risch, Anita C. Schuetz, Martin Speziale, Karina L. Standish, Rachel J. Sullivan, Lauren L. Wardle, Glenda M. Williams, Ryan J. Yang, Louie H. TI Comment on "Worldwide evidence of a unimodal relationship between productivity and plant species richness" SO SCIENCE LA English DT Editorial Material C1 [Tredennick, Andrew T.; Adler, Peter B.] Utah State Univ, Dept Wildland Resources, 5230 Old Main, Logan, UT 84322 USA. [Tredennick, Andrew T.; Adler, Peter B.] Utah State Univ, Ctr Ecol, 5230 Old Main, Logan, UT 84322 USA. [Grace, James B.] US Geol Survey, Wetland & Aquat Res Ctr, 700 Cajundome Blvd, Lafayette, LA 70506 USA. [Harpole, W. Stanley] UFZ Helmholtz Ctr Environm Res, Dept Physiol Divers, Permoserstr 15, D-04318 Leipzig, Germany. [Borer, Elizabeth T.; Lind, Eric M.; Sullivan, Lauren L.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Anderson, T. Michael] Wake Forest Univ, Dept Biol, Box 7325 Reynolda Stn, Winston Salem, NC 27109 USA. [Bakker, Jonathan D.] Univ Washington, Sch Environm & Forest Sci, 3501 NE 41st St,Box 354115, Seattle, WA 98195 USA. [Biederman, Lori A.] Iowa State Univ, Ecol Evolut & Organismal Biol, 251 Bessey Hall, Ames, IA 50010 USA. [Brown, Cynthia S.] Colorado State Univ, Dept Bioagr Sci & Pest Management, 307 Univ Ave, Ft Collins, CO 80523 USA. [Buckley, Yvonne M.] Univ Dublin, Trinity Coll Dublin, Sch Nat Sci, Zool, Dublin 2, Ireland. [Chu, Chengjin] Sun Yat Sen Univ, Sch Life Sci, Xingang Xi Rd 135, Guangzhou 510275, Guangdong, Peoples R China. [Collins, Scott L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Crawley, Michael J.] Univ London Imperial Coll Sci Technol & Med, Dept Biol, Silwood Pk, Ascot SL5 7PY, Berks, England. [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Firn, Jennifer] Queensland Univ Technol, Sch Earth Environm & Biol Sci 42, Brisbane, Qld 4001, Australia. [Gruner, Daniel S.] Univ Maryland, Dept Entomol, Plant Sci 4112, College Pk, MD 20742 USA. [Hagenah, Nicole; Kirkman, Kevin] Univ KwaZulu Natal, Sch Life Sci, 1 Carbis Rd, ZA-3201 Pietermaritzburg, South Africa. [Hautier, Yann] Univ Utrecht, Dept Biol, Ecol & Biodivers Grp, Padualaan 8, NL-3584 CH Utrecht, Netherlands. [Hector, Andy] Univ Oxford, Dept Plant Sci, S Parks Rd, Oxford OX1 3RB, England. [Hillebrand, Helmut] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm, Schleusenstr 1, D-26382 Wihlhemshaven, Germany. [Knops, Johannes M. H.] Univ Nebraska, Sch Biol Sci, 211 Manter Hall, Lincoln, NE 68588 USA. [Laungani, Ramesh] Doane Coll, Dept Biol, 1014 Boswell Ave, Crete, NE 68333 USA. [MacDougall, Andrew S.] Univ Guelph, Dept Integrat Biol, 50 Stone Rd, Guelph, ON N1G 2W1, Canada. [McCulley, Rebecca L.] Univ Kentucky, Dept Plant & Soil Sci, N-222D Ag Sci North, Lexington, KY 40546 USA. [Mitchell, Charles E.] Univ N Carolina, Dept Biol, CB 3280, Chapel Hill, NC 27599 USA. [Moore, Joslin L.] Monash Univ, Sch Biol Sci, Clayton Campus,Wellington Rd, Clayton, Vic 3800, Australia. [Morgan, John W.] La Trobe Univ, Dept Ecol Environm & Evolut, Kingsbury Dr, Bundoora, Vic 3086, Australia. [Orrock, John L.] Univ Wisconsin, Dept Zool, 430 Lincoln Dr, Madison, WI 53706 USA. [Peri, Pablo L.] Southern Patagonia Natl Univ, Dept Forestry Agr & Water, INTA, CONICET, CC 332, RA-9400 Santa Cruz, Patagonia, Argentina. [Prober, Suzanne M.] Commonwealth Sci & Ind Res Org Land & Water, Private Bag 5, Wembley, WA 6913, Australia. [Risch, Anita C.; Schuetz, Martin] Swiss Fed Inst Forest Snow & Landscape Res, Community Ecol, Zuercherstr 111, CH-8903 Birmensdorf, Switzerland. [Speziale, Karina L.] UNCO, CONICET, Dept Ecol, INIBIOMA, Quintral 1250, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. [Standish, Rachel J.] Murdoch Univ, Sch Vet & Life Sci, 90 South St, Murdoch, WA 6150, Australia. [Wardle, Glenda M.] Univ Sydney, Sch Biol Sci, Heydon Laurence Bldg A08, Sydney, NSW 2006, Australia. [Williams, Ryan J.] Iowa State Univ, Agr & Biosyst Engn, Ames, IA USA. [Yang, Louie H.] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA. RP Tredennick, AT (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main, Logan, UT 84322 USA.; Tredennick, AT (reprint author), Utah State Univ, Ctr Ecol, 5230 Old Main, Logan, UT 84322 USA. EM atredenn@gmail.com RI Gruner, Daniel/A-5166-2010; MacDougall, Andrew/F-2037-2011; Hector, Andrew/H-4199-2011; Risch, Anita/A-9836-2012; Standish, Rachel/B-1028-2011; Hautier, Yann/D-5426-2015; Pillar, Valerio/B-9872-2008; Hillebrand, Helmut/I-1717-2014; Prober, Suzanne/G-6465-2010; OI Gruner, Daniel/0000-0002-3153-4297; Lind, Eric/0000-0003-3051-7724; Borer, Elizabeth/0000-0003-2259-5853; Hector, Andrew/0000-0002-1309-7716; Risch, Anita/0000-0003-0531-8336; Hautier, Yann/0000-0003-4347-7741; Pillar, Valerio/0000-0001-6408-2891; Hillebrand, Helmut/0000-0001-7449-1613; Biederman, Lori/0000-0003-2171-7898 NR 6 TC 2 Z9 2 U1 12 U2 51 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JAN 29 PY 2016 VL 351 IS 6272 DI 10.1126/science.aad6236 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB9SW UT WOS:000368858200024 PM 26823418 ER PT J AU Tassone, EE Geib, SM Hall, B Fabrick, JA Brent, CS Hull, JJ AF Tassone, Erica E. Geib, Scott M. Hall, Brian Fabrick, Jeffrey A. Brent, Colin S. Hull, J. Joe TI De novo construction of an expanded transcriptome assembly for the western tarnished plant bug, Lygus hesperus SO GIGASCIENCE LA English DT Article DE Transcriptome; Lygus hesperus; Plant bug; Miridae; RNA-Seq; Trinity ID GENOME AB Background: The plant bug Lygus hesperus Knight is a polyphagous pest of many economically important crops. Despite its pest status, little is known about the molecular mechanisms responsible for much of the biology of this species. Earlier Lygus transcriptome assemblies were limited by low read depth, or because they focused on specific conditions. To generate a more comprehensive transcriptome, we supplemented previous datasets with new reads corresponding to specific tissues (heads, antennae, and male reproductive tissues). This transcriptome augments current Lygus molecular resources and provides the foundational knowledge critical for future comparative studies. Findings: An expanded, Trinity/based de novo transcriptome assembly for L. hesperus was generated using previously published whole body Illumina data, supplemented with 293 million bp of new raw sequencing data corresponding to five tissue-specific cDNA libraries and 11 Illumina sequencing runs. The updated transcriptome consists of 22,022 transcripts (average length of 2075 nt), 62 % of which contain complete open reading frames. Significant coverage of the BUSCO (benchmarking universal single-copy orthologs) dataset and robust metrics indicate that the transcriptome is a quality assembly with a high degree of completeness. Initial assessment of the new assembly's utility revealed that the length and abundance of transcripts predicted to regulate insect physiology and chemosensation have improved, compared with previous L. hesperus assemblies. Conclusions: This transcriptome represents a significant expansion of Lygus transcriptome data, and improves foundational knowledge about the molecular mechanisms underlying L. hesperus biology. The dataset is publically available in NCBI and GigaDB as a resource for researchers. C1 [Fabrick, Jeffrey A.; Brent, Colin S.; Hull, J. Joe] USDA ARS, Pest Management & Biocontrol Res Unit, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Tassone, Erica E.] USDA ARS, Plant Physiol & Genet Res Unit, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Geib, Scott M.; Hall, Brian] USDA ARS, Trop Crop & Commod Protect Res Unit, Daniel K Inouye Pacific Basin Agr Res Ctr, Hilo, HI USA. [Hall, Brian] Univ Hawaii Manoa, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA. RP Hull, JJ (reprint author), USDA ARS, Pest Management & Biocontrol Res Unit, US Arid Land Agr Res Ctr, Maricopa, AZ USA. EM joe.hull@ars.usda.gov FU Cotton Inc. (CSB) [12-373]; National Science Foundation [OCI-1053575XSEDE, TG-MCB140032] FX The authors thank both Daniel Langhorst (ALARC) and Lynn Forlow Jech (ALARC) for technical support, as well as Brooks Silversmith (ALARC) and Anna Cervantes (ALARC) for maintaining the L. hesperus colony. The research described in this manuscript was partially supported by funds from Cotton Inc. (CSB, project no. 12-373). Bioinformatic analysis was performed on computing resources at the USDA-ARS Daniel K. Inouye Pacific Basin Agricultural Research Center (Moana cluster; Hilo, HI) and the Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by National Science Foundation grant number OCI-1053575XSEDE under allocation TG-MCB140032 to SMG. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 16 TC 1 Z9 1 U1 4 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND EI 2047-217X J9 GIGASCIENCE JI GigaScience PD JAN 28 PY 2016 VL 5 AR 6 DI 10.1186/s13742-016-0109-6 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC7JM UT WOS:000369396000004 PM 26823975 ER PT J AU Margevicius, KJ Generous, N Abeyta, E Althouse, B Burkom, H Castro, L Daughton, A Del Valle, SY Fairchild, G Hyman, JM Kiang, R Morse, AP Pancerella, CM Pullum, L Ramanathan, A Schlegelmilch, J Scott, A Taylor-McCabe, KJ Vespignani, A Deshpande, A AF Margevicius, Kristen J. Generous, Nicholas Abeyta, Esteban Althouse, Ben Burkom, Howard Castro, Lauren Daughton, Ashlynn Del Valle, Sara Y. Fairchild, Geoffrey Hyman, James M. Kiang, Richard Morse, Andrew P. Pancerella, Carmen M. Pullum, Laura Ramanathan, Arvind Schlegelmilch, Jeffrey Scott, Aaron Taylor-McCabe, Kirsten J. Vespignani, Alessandro Deshpande, Alina TI The Biosurveillance Analytics Resource Directory (BARD): Facilitating the Use of Epidemiological Models for Infectious Disease Surveillance SO PLOS ONE LA English DT Article ID DECISION-MAKING; POLICY; REAL; TOOL AB Epidemiological modeling for infectious disease is important for disease management and its routine implementation needs to be facilitated through better description of models in an operational context. A standardized model characterization process that allows selection or making manual comparisons of available models and their results is currently lacking. A key need is a universal framework to facilitate model description and understanding of its features. Los Alamos National Laboratory (LANL) has developed a comprehensive framework that can be used to characterize an infectious disease model in an operational context. The framework was developed through a consensus among a panel of subject matter experts. In this paper, we describe the framework, its application to model characterization, and the development of the Biosurveillance Analytics Resource Directory (BARD; http://brd.bsvgateway.org/brd/), to facilitate the rapid selection of operational models for specific infectious/communicable diseases. We offer this framework and associated database to stakeholders of the infectious disease modeling field as a tool for standardizing model description and facilitating the use of epidemiological models. C1 [Margevicius, Kristen J.; Generous, Nicholas; Abeyta, Esteban; Castro, Lauren; Daughton, Ashlynn; Del Valle, Sara Y.; Fairchild, Geoffrey; Taylor-McCabe, Kirsten J.; Deshpande, Alina] Los Alamos Natl Lab, Analyt Intelligence & Technol Div, Los Alamos, NM USA. [Althouse, Ben] Santa Fe Inst, Santa Fe, NM 87501 USA. [Burkom, Howard] Johns Hopkins Univ, Appl Phys Lab, Laurel, MD USA. [Hyman, James M.] Tulane Univ, Dept Math, New Orleans, LA 70118 USA. [Kiang, Richard] Natl Aeronaut & Space Adm, Greenbelt, MD USA. [Morse, Andrew P.] Univ Liverpool, Sch Environm Sci, Dept Geog & Planning, Liverpool L69 3BX, Merseyside, England. [Morse, Andrew P.] NIHR Hlth Protect Res Unit Emerging & Zoonot Infe, Liverpool, Merseyside, England. [Pancerella, Carmen M.] Sandia Natl Labs, Distributed Syst Res, Livermore, CA USA. [Pullum, Laura; Ramanathan, Arvind] Oak Ridge Natl Lab, Oak Ridge, TN USA. [Schlegelmilch, Jeffrey] Columbia Univ, Earth Inst, Natl Ctr Disaster Preparedness, New York, NY USA. [Scott, Aaron] USDA APHIS Vet Serv, Sci, Technol, Anal Serv, Ft Collins, CO USA. [Vespignani, Alessandro] Northeastern Univ, Lab Modeling Biol & Sociotech Syst, Boston, MA 02115 USA. RP Deshpande, A (reprint author), Los Alamos Natl Lab, Analyt Intelligence & Technol Div, Los Alamos, NM USA. EM Deshpande_a@lanl.gov OI burkom, howard/0000-0003-0667-9467; Margevicius, Kristen/0000-0002-4116-8307 FU Defense Threat Reductions Agency - Joint Science and Technology Office [CB10007, DTRA10027-10845] FX This project was funded by the Defense Threat Reductions Agency - Joint Science and Technology Office (Grant # CB10007, DTRA10027-10845). NR 29 TC 0 Z9 0 U1 3 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 28 PY 2016 VL 11 IS 1 AR e0146600 DI 10.1371/journal.pone.0146600 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9GF UT WOS:000369528400008 PM 26820405 ER PT J AU Jiang, H Kim, D Dobesh, S Evans, JD Nachman, RJ Kaczmarek, K Zabrocki, J Park, Y AF Jiang, Hongbo Kim, Donghun Dobesh, Sharon Evans, Jay D. Nachman, Ronald J. Kaczmarek, Krzystof Zabrocki, Janusz Park, Yoonseong TI Ligand selectivity in tachykinin and natalisin neuropeptidergic systems of the honey bee parasitic mite Varroa destructor SO SCIENTIFIC REPORTS LA English DT Article ID TICK IXODES-SCAPULARIS; FUNCTIONAL-CHARACTERIZATION; INSECT NEUROPEPTIDES; LOCUSTATACHYKININ-I; DIAPAUSE HORMONE; SALIVARY-GLANDS; PEPTIDES; DROSOPHILA; RECEPTOR; VIRUS AB The varroa mite, Varroa destructor, is a devastating ectoparasite of the honey bees Apis mellifera and A. cerana. Control of these mites in beehives is a challenge in part due to the lack of toxic agents that are specific to mites and not to the host honey bee. In searching for a specific toxic target of varroa mites, we investigated two closely related neuropeptidergic systems, tachykinin-related peptide (TRP) and natalisin (NTL), and their respective receptors. Honey bees lack both NTL and the NTL receptor in their genome sequences, providing the rationale for investigating these receptors to understand their specificities to various ligands. We characterized the receptors for NTL and TRP of V. destructor (VdNTL-R and VdTRP-R, respectively) and for TRP of A. mellifera (AmTRP-R) in a heterologous reporter assay system to determine the activities of various ligands including TRP/NTL peptides and peptidomimetics. Although we found that AmTRP-R is highly promiscuous, activated by various ligands including two VdNTL peptides when a total of 36 ligands were tested, we serendipitously found that peptides carrying the C-terminal motif-FWxxRamide are highly specific to VdTRP-R. This motif can serve as a seed sequence for designing a VdTRP-R-specific agonist. C1 [Jiang, Hongbo; Kim, Donghun; Dobesh, Sharon; Park, Yoonseong] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Jiang, Hongbo] Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing 400715, Peoples R China. [Evans, Jay D.] USDA ARS, Bee Res Lab, BARC E, Beltsville, MD 20705 USA. [Nachman, Ronald J.; Kaczmarek, Krzystof; Zabrocki, Janusz] USDA, Insect Control & Cotton Dis Res Unit, Southern Plains Agr Res Ctr, 2881 F-B Rd, College Stn, TX 77845 USA. [Kaczmarek, Krzystof; Zabrocki, Janusz] Lodz Univ Technol, Inst Organ Chem, PL-90924 Lodz, Poland. RP Park, Y (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM ypark@ksu.edu OI Evans, Jay/0000-0002-0036-4651 FU National institute of health [R01AI090062]; USDA/DOD DWFP [60-0208-4-001]; Fundamental Research Funds for Central Universities of China [XDJK2015A008, 2362015xk04]; Kansas State University Open Access Publishing Fund FX This paper is contribution no. 16-190-J from the Kansas Agricultural Experiment Station. HJ and YP were supported in part by National institute of health; Grant Number: R01AI090062; RJN was supported in part by a grants from USDA/DOD DWFP (60-0208-4-001). This project was supported in part by the Fundamental Research Funds for the Central Universities of China (XDJK2015A008, 2362015xk04). Publication of this article was funded in part by the Kansas State University Open Access Publishing Fund. NR 27 TC 0 Z9 0 U1 7 U2 26 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JAN 28 PY 2016 VL 6 AR 19547 DI 10.1038/srep19547 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB8QT UT WOS:000368782300001 PM 26817786 ER PT J AU Sobolev, VS Krausert, NM Gloer, JB AF Sobolev, Victor S. Krausert, Nicole M. Gloer, James B. TI New Monomeric Stilbenoids from Peanut (Arachis hypogaea) Seeds Challenged by an Aspergillus flavus Strain SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE peanuts; Arachis hypogaea; arahypin; groundnuts; stilbenes; prenylated stilbenes; stilbenoids; aflatoxin; structure elucidation; NMR; HPLC-MS ID INHIBITORY-ACTIVITY; PHYTOALEXIN; RESISTANCE; BARK AB Two new stilbene derivatives have been isolated from peanut seeds challenged by an Aspergillus flavus strain, along with chiricanine B, which has not been previously reported from peanuts, as well as a stilbenoid reported previously only as a synthetic product. The structures of these new putative phytoalexins were determined by analysis of H-1 and C-13 NMR, HRESIMS, MSn, and UV data. The new stilbenoids were named arahypin-13 (21), arahypin-14 (22), and arahypin-15 (23). Together with other known bioactive peanut stilbenoids that were also produced in the challenged seeds, these new compounds may play a defensive role against invasive fungi. C1 [Sobolev, Victor S.] ARS, Natl Peanut Res Lab, USDA, POB 509, Dawson, GA 39842 USA. [Krausert, Nicole M.; Gloer, James B.] Univ Iowa, Dept Chem, 230 North Madison St, Iowa City, IA 52242 USA. RP Sobolev, VS (reprint author), ARS, Natl Peanut Res Lab, USDA, POB 509, Dawson, GA 39842 USA. EM victor.sobolev@ars.usda.gov NR 20 TC 3 Z9 3 U1 1 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 27 PY 2016 VL 64 IS 3 BP 579 EP 584 DI 10.1021/acs.jafc.5b04753 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DC2JY UT WOS:000369044200006 PM 26672388 ER PT J AU Liu, N Guo, QF Ren, H Sun, ZY AF Liu, Nan Guo, Qinfeng Ren, Hai Sun, Zhongyu TI Schima superba outperforms other tree species by changing foliar chemical composition and shortening construction payback time when facilitated by shrubs SO SCIENTIFIC REPORTS LA English DT Article ID TROPICAL RAIN-FOREST; RELATIVE GROWTH-RATE; NURSE PLANTS; LEAF CONSTRUCTION; RHODOMYRTUS-TOMENTOSA; USE EFFICIENCY; LOW-IRRADIANCE; RAPID METHOD; PICEA-ABIES; LEAVES AB A 3.5-year field experiment was conducted in a subtropical degraded shrubland to assess how a nurse plant, the native shrub Rhodomyrtus tomentosa, affects the growth of the target trees Pinus elliottii, Schima superba, Castanopsis fissa, and Michelia macclurei, and to probe the intrinsic mechanisms from leaf chemical composition, construction cost (CC), and payback time aspects. We compared tree seedlings grown nearby shrub canopy (canopy subplots, CS) and in open space (open subplots, OS). S. superba in CS showed greater growth, while P. elliottii and M. macclurei were lower when compared to the plants grown in the OS. The reduced levels of high-cost compounds (proteins) and increased levels of low-cost compounds (organic acids) caused reduced CC values for P. elliottii growing in CS. While, the levels of both low-cost minerals and high-cost proteins increased in CS such that CC values of S. superba were similar in OS and CS. Based on maximum photosynthetic rates, P. elliottii required a longer payback time to construct required carbon in canopy than in OS, but the opposite was true for S. superba. The information from this study can be used to evaluate the potential of different tree species in the reforestation of subtropical degraded shrublands. C1 [Liu, Nan; Ren, Hai] Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China. [Guo, Qinfeng] USDA FS, Eastern Forest Environm Threat Assessment Ctr, Res Triangle Pk, NC 27709 USA. [Sun, Zhongyu] Guangzhou Inst Geog, Guangdong Open Lab Geospatial Informat Technol &, Guangzhou 510070, Guangdong, Peoples R China. RP Ren, H (reprint author), Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China. EM renhai@scbg.ac.cn FU National Natural Science Foundation of China [31270013, 30901148]; Youth Innovation Promotion Association of the Chinese Academy of Sciences; Guangdong Science and Technology Planning Project [2015A030303014, 2014A030305014] FX This research was supported by the National Natural Science Foundation of China (31270013; 30901148), Youth Innovation Promotion Association of the Chinese Academy of Sciences, and Guangdong Science and Technology Planning Project (2015A030303014; 2014A030305014). We are grateful to our colleagues in the Heshan National Field Research Station of Forest Ecosystems. We also thank Bruce Jaffee for language editing, Lanlan Guan and Huilan Zhang for laboratory analyses, and Long Yang, and Zhipeng Chen for field assistances. NR 60 TC 0 Z9 0 U1 8 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JAN 27 PY 2016 VL 6 AR 19855 DI 10.1038/srep19855 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB7BM UT WOS:000368669400002 PM 26814426 ER PT J AU Ma, JT McKeown, NM Hwang, SJ Hoffmann, U Jacques, PF Fox, CS AF Ma, Jiantao McKeown, Nicola M. Hwang, Shih-Jen Hoffmann, Udo Jacques, Paul F. Fox, Caroline S. TI Sugar-Sweetened Beverage Consumption Is Associated With Change of Visceral Adipose Tissue Over 6 Years of Follow-Up SO CIRCULATION LA English DT Article DE obesity; subcutaneous adipose tissue; visceral adipose tissue ID FOOD-FREQUENCY QUESTIONNAIRE; LIPOPROTEIN-LIPASE; METABOLIC SYNDROME; UNITED-STATES; FRUCTOSE; DISEASE; FAT; REPRODUCIBILITY; VALIDITY; INSULIN AB Background Sugar-sweetened beverage (SSB) intake has been linked to abnormal abdominal adipose tissue. We examined the prospective association of habitual SSB intake and change in visceral adipose tissue (VAT) and subcutaneous adipose tissue. Methods and Results The quantity (volume, cm(3)) and quality (attenuation, Hounsfield Unit) of abdominal adipose tissue were measured using computed tomography in 1003 participants (mean age 45.3 years, 45.0% women) at examination 1 and 2 in the Framingham's Third Generation cohort. The 2 exams were approximate to 6 years apart. At baseline, SSB and diet soda intake were assessed using a valid food frequency questionnaire. Participants were categorized into 4 groups: none to <1 serving/mo (nonconsumers), 1 serving/mo to <1 serving/week, 1 serving/week to 1 serving/d, and 1 serving/d (daily consumers) of either SSB or diet soda. After adjustment for multiple confounders including change in body weight, higher SSB intake was associated with greater change in VAT volume (P trend<0.001). VAT volume increased by 658 cm(3) (95% confidence interval [CI], 602 to 713), 649 cm(3) (95% CI, 582 to 716), 707 cm(3) (95% CI, 657 to 757), and 852 cm(3) (95% CI, 760 to 943) from nonconsumers to daily consumers. Higher SSB intake was also associated with greater decline of VAT attenuation (P trend=0.007); however, the association became nonsignificant after additional adjustment for VAT volume change. In contrast, diet soda consumption was not associated with change in abdominal adipose tissue. Conclusions Regular SSB intake was associated with adverse change in both VAT quality and quantity, whereas we observed no such association for diet soda. C1 [Ma, Jiantao; Hwang, Shih-Jen; Fox, Caroline S.] NHLBI, Framingham Heart Study & Populat Sci Branch, 73 Mt Wayte Ave,Suite 2, Framingham, MA 01702 USA. [McKeown, Nicola M.; Jacques, Paul F.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Hoffmann, Udo] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Radiol, Boston, MA USA. RP Fox, CS (reprint author), NHLBI, Framingham Heart Study & Populat Sci Branch, 73 Mt Wayte Ave,Suite 2, Framingham, MA 01702 USA. EM foxa@nhlbi.nih.gov FU Framingham Heart Study (FHS) of National Heart, Lung, and Blood Institute (NHLBI) of National Institute of Health; Boston University School of Medicine; NHLBI FHS [N01-HC-25195] FX This work was conducted in part using resources and data from the Framingham Heart Study (FHS) of the National Heart, Lung, and Blood Institute (NHLBI) of the National Institute of Health and Boston University School of Medicine. This work was supported by the NHLBI FHS (N01-HC-25195). NR 35 TC 2 Z9 2 U1 3 U2 7 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0009-7322 EI 1524-4539 J9 CIRCULATION JI Circulation PD JAN 26 PY 2016 VL 133 IS 4 BP 370 EP 377 DI 10.1161/CIRCULATIONAHA.115.018704 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA DC5KE UT WOS:000369259100005 PM 26755505 ER PT J AU Snow, NP Halseth, JM Lavelle, MJ Hanson, TE Blass, CR Foster, JA Humphrys, ST Staples, LD Hewitt, DG VerCauteren, KC AF Snow, Nathan P. Halseth, Joseph M. Lavelle, Michael J. Hanson, Thomas E. Blass, Chad R. Foster, Justin A. Humphrys, Simon T. Staples, Linton D. Hewitt, David G. VerCauteren, Kurt C. TI Bait Preference of Free-Ranging Feral Swine for Delivery of a Novel Toxicant SO PLOS ONE LA English DT Article ID BOAR SUS-SCROFA; SOUTHERN TEXAS; UNITED-STATES; WILD PIGS; BIRDS; PHARMACEUTICALS; CONSUMPTION; BEHAVIOR; COLOR; HOGS AB Invasive feral swine (Sus scrofa) cause extensive damage to agricultural and wildlife resources throughout the United States. Development of sodium nitrite as a new, orally delivered toxicant is underway to provide an additional tool to curtail growth and expansion of feral swine populations. A micro-encapsulation coating around sodium nitrite is used to minimize detection by feral swine and maximize stability for the reactive molecule. To maximize uptake of this toxicant by feral swine, development a bait matrix is needed to 1) protect the micro-encapsulation coating so that sodium nitrite remains undetectable to feral swine, 2) achieve a high degree of acceptance by feral swine, and 3) be minimally appealing to non-target species. With these purposes, a field evaluation at 88 sites in south-central Texas was conducted using remote cameras to evaluate preferences by feral swine for several oil-based bait matrices including uncolored peanut paste, black-colored peanut paste, and peanut-based slurry mixed onto whole-kernel corn. These placebo baits were compared to a reference food, whole-kernel corn, known to be readily taken by feral swine (i.e., control). The amount of bait consumed by feral swine was also estimated using remote cameras and grid boards at 5 additional sites. On initial exposure, feral swine showed reduced visitations to the uncolored peanut paste and peanut slurry treatments. This reduced visitation subsided by the end of the treatment period, suggesting that feral swine needed time to accept these bait types. The black-colored peanut paste was visited equally to the control throughout the study, and enough of this matrix was consumed to deliver lethal doses of micro-encapsulated sodium nitrite to most feral swine during 1-2 feeding events. None of the treatment matrices reduced visitations by nontarget species, but feral swine dominated visitations for all matrices. It was concluded that black-colored peanut paste achieved satisfactory preference and consumption by feral swine, and no discernable preference by non-target species, compared to the other treatments. C1 [Snow, Nathan P.; Hewitt, David G.] Texas A&M Univ, Caesar Kleberg Wildlife Res Inst, 700 Univ Blvd,MSC 218, Kingsville, TX 78363 USA. [Snow, Nathan P.; Halseth, Joseph M.; Lavelle, Michael J.; Hanson, Thomas E.; Blass, Chad R.; VerCauteren, Kurt C.] US Fish & Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Foster, Justin A.] Texas Parks & Wildlife Dept, Kerr Wildlife Management Area, 2625 FM 1340, Hunt, TX 78024 USA. [Humphrys, Simon T.; Staples, Linton D.] Invas Anim CRC, 33 Flemington St, Glenside, SA 5062, Australia. [Staples, Linton D.] Anim Control Technol Australia Pty Ltd, 46-50 Freight Dr, Somerton, Vic 3062, Australia. RP Snow, NP (reprint author), Texas A&M Univ, Caesar Kleberg Wildlife Res Inst, 700 Univ Blvd,MSC 218, Kingsville, TX 78363 USA.; Snow, NP (reprint author), US Fish & Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Nathan.P.Snow@aphis.usda.gov FU USDA National Feral Swine Damage Management Program; Invasive Animals Cooperative Research Center FX Funding was provided by the USDA National Feral Swine Damage Management Program and the Invasive Animals Cooperative Research Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. One author (LS) of this manuscript was commercially affiliated with Animal Control Technologies Australia Pty Ltd. This author played a role in the study by providing the test materials and preparation of the manuscript. NR 48 TC 1 Z9 1 U1 4 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 26 PY 2016 VL 11 IS 1 AR e0146712 DI 10.1371/journal.pone.0146712 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9GB UT WOS:000369528000012 PM 26812148 ER PT J AU Wang, D Gabriel, MZ Legard, D Sjulin, T AF Wang, D. Gabriel, M. Z. Legard, D. Sjulin, T. TI Characteristics of growing media mixes and application for open-field production of strawberry (Fragaria ananassa) SO SCIENTIA HORTICULTURAE LA English DT Article DE Substrate media; Soilless strawberry cultivation; Water retention; TDR ID TIME-DOMAIN REFLECTOMETRY; MEASURING WATER-CONTENT; PHYSICAL-PROPERTIES; HYDRAULIC CHARACTERISTICS; GROWTH MEDIA; SOIL; YIELD; PEAT; IRRIGATION; GREENHOUSE AB Growing media are widely used for strawberry production in protected environments. Due to increasing restrictions on the use of soil fumigants, there is a need to investigate production of strawberry fruits in open fields using substrate media. The objectives of the study were to characterize chemical and hydraulic properties of a number of selected growing media mixes and to evaluate them in a two-year field study for strawberry production in open fields. The growing media mixes were 100% coconut coir, a peat-perlite (PP) mix, a peat-rice hull (PR) mix, and a peat-coir-rice hull (PCR) mix. Results indicated that major macro- and micro nutrient element concentrations, pH, and salinity found in these growing media, after supplemental fertilization, were in recommended ranges for strawberry production. Compared to field soil, higher water content values were found for these growing media mixes at saturation and after free drainage. Among these media mixes, 100% Coir showed the highest water content values at saturation (0.95 cm(3) cm(-3)) and after free drainage (0.51 cm(3) cm(-3)). The field study also demonstrated the most vigorous vegetative growth in Coir by showing higher values in plant canopy size and biomass dry weight. In year 1 field experiment, marketable strawberry fruit yields from Coir, PP, and PR averaged 41,640 kg ha(-1) which was not significantly different from the soil beds. Marketable strawberry yields from the year 2 Coir and PP treatments averaged 48,520 kg ha(-1), which was also not significantly different from the grower standard practice in field soils. The year 2 yield was higher than year 1 because of longer harvesting season and more favorable weather conditions for strawberry growth. The study demonstrated that certain growing media mixes such as PP or the 100% Coir possess the needed chemical and hydraulic properties for plant growth and the 100% Coir could be used as a promising substrate material for open-field production of strawberries. Additional work is needed to optimize the irrigation and fertilization regimes to reduce the volume of growing media needed per plant and to evaluate the reuse of the substrate media to reduce production cost. Published by Elsevier B.V. C1 [Wang, D.; Gabriel, M. Z.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Gabriel, M. Z.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Legard, D.] Calif Strawberry Commiss, Watsonville, CA 95076 USA. [Sjulin, T.] Hort Consulting, Aromas, CA 95004 USA. [Gabriel, M. Z.] Marrone Bio Innovat Inc, Davis, CA 95618 USA. RP Wang, D (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM dong.wang@ars.usda.gov NR 46 TC 0 Z9 0 U1 16 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JAN 26 PY 2016 VL 198 BP 294 EP 303 DI 10.1016/j.scienta.2015.11.023 PG 10 WC Horticulture SC Agriculture GA DC8HL UT WOS:000369460400037 ER PT J AU Imahori, Y Bai, JH Baldwin, E AF Imahori, Yoshihiro Bai, Jinhe Baldwin, Elizabeth TI Antioxidative responses of ripe tomato fruit to postharvest chilling and heating treatments SO SCIENTIA HORTICULTURAE LA English DT Article DE Antioxidant reductive capacity; Ascorbate-glutathione cycle; Chilling; Heating; Malondialdehyde; Tomato fruit ID HYDROGEN-PEROXIDE CONTENT; LOW-TEMPERATURE STORAGE; OXIDATIVE STRESS; ASCORBATE PEROXIDASE; ENZYME-ACTIVITIES; PLANT-RESPONSES; FREE-RADICALS; TOLERANCE; INJURY; LIPOXYGENASE AB The objective of this research was to determine the antioxidative responses to chilling and heating stresses in ripe tomatoes cv. 'Sanibel'. Full ripe fruit were treated with either chilling (5 degrees C for 5 days), hot water (52 degrees C for 15 min, then cooled to 25 degrees C with tap water), or left untreated as the control. Fruit samples were taken directly after treatment or after 4 days storage at 20 degrees C. Directly after treatments, heating remarkably increased the activity of lipoxygenase (LOX), and chilling increased malondialdehyde (MDA) content. LOX catalyzes the oxidation of lipids, and MDA is a compound produced from lipids under oxidative stress. The increase of LOX activity and MDA content indicates escalated reactive oxygen species (ROS) in the tissues. The heat treatment also increased ascorbate levels, and induced the activities of catalase (CAT) and peroxidase (POD) as well. After 4 days storage at 20 degrees C, antioxidant reductive capacity (ARC) and the activity of ascorbate peroxidase (APX) was significantly higher in the chilling treated fruit than in the control. Chilling directly increased MDA and ascorbate levels, and led to a remarkable increase in H2O2 on day 4 after treatment. The activities of CAT and POD were lower in chilled fruit than in the control on day 4 after treatment. These results indicate that oxidative stress may be induced by chilling treatment even without visual injury on the fruit surface. Heat treatment enhanced the antioxidant enzyme system to potentially protect fruit from environment stress. (C) 2015 Elsevier B.V. All rights reserved. C1 [Imahori, Yoshihiro] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Lab Postharvest Physiol, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan. [Bai, Jinhe; Baldwin, Elizabeth] USDA ARS, Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP Imahori, Y (reprint author), Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Lab Postharvest Physiol, 1-1 Gakuen Cho, Sakai, Osaka 5998531, Japan. EM imahori@plant.osakafu-u.ac.jp NR 58 TC 0 Z9 1 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JAN 26 PY 2016 VL 198 BP 398 EP 406 DI 10.1016/j.scienta.2015.12.006 PG 9 WC Horticulture SC Agriculture GA DC8HL UT WOS:000369460400049 ER PT J AU Patterson, SE Bolivar-Medina, JL Falbel, TG Hedtcke, JL Nevarez-McBride, D Maule, AF Zalapa, JE AF Patterson, Sara E. Bolivar-Medina, Jenny L. Falbel, Tanya G. Hedtcke, Janet L. Nevarez-McBride, Danielle Maule, Andrew F. Zalapa, Juan E. TI Are We on the Right Track: Can Our Understanding of Abscission in Model Systems Promote or Derail Making Improvements in Less Studied Crops? SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE abscission; shedding; seed-shatter; grape; cranberry; fonio; tomato ID FLORAL ORGAN ABSCISSION; CELL-SEPARATION PROCESSES; TOMATO FLOWER ABSCISSION; MADS-BOX GENE; ARABIDOPSIS-THALIANA; INFLORESCENCE-DEFICIENT; TRANSCRIPTION FACTOR; RICE DOMESTICATION; ZONE CELLS; EXPRESSION AB As the world population grows and resources and climate conditions change, crop improvement continues to be one of the most important challenges for agriculturalists. The yield and quality of many crops is affected by abscission or shattering, and environmental stresses often hasten or alter the abscission process. Understanding this process can not only lead to genetic improvement, but also changes in cultural practices and management that will contribute to higher yields, improved quality and greater sustainability. As plant scientists, we have learned significant amounts about this process through the study of model plants such as Arabidopsis, tomato, rice, and maize. While these model systems have provided significant valuable information, we are sometimes challenged to use this knowledge effectively as variables including the economic value of the crop, the uniformity of the crop, ploidy levels, flowering and crossing mechanisms, ethylene responses, cultural requirements, responses to changes in environment, and cellular and tissue specific morphological differences can significantly influence outcomes. The value of genomic resources for lesser-studied crops such as cranberries and grapes and the orphan crop fonio will also be considered. C1 [Patterson, Sara E.; Bolivar-Medina, Jenny L.; Falbel, Tanya G.; Nevarez-McBride, Danielle; Maule, Andrew F.; Zalapa, Juan E.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Bolivar-Medina, Jenny L.; Zalapa, Juan E.] ARS, Vegetable Crops Res Unit, USDA, Madison, WI USA. [Hedtcke, Janet L.] West Madison Agr Res Stn, Verona, WI USA. RP Patterson, SE (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM spatters@wisc.edu FU College of Agricultural and Life Sciences; Graduate School of the University of Wisconsin; NSF [1250868/1063085, SCBG-14-004] FX This research was supported by the College of Agricultural and Life Sciences and the Graduate School of the University of Wisconsin, and by grants from NSF 1250868/1063085 and SCBG-14-004. NR 83 TC 0 Z9 0 U1 2 U2 12 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JAN 26 PY 2016 VL 6 AR 1268 DI 10.3389/fpls.2015.01268 PG 8 WC Plant Sciences SC Plant Sciences GA DB8LF UT WOS:000368767500001 ER PT J AU Das, M Sha, J Hidalgo, B Aslibekyan, S Do, AN Zhi, DG Sun, DJY Zhang, T Li, SX Chen, W Srinivasan, SR Tiwari, HK Absher, D Ordovas, JM Berenson, GS Arnett, DK Irvin, MR AF Das, Mithun Sha, Jin Hidalgo, Bertha Aslibekyan, Stella Do, Anh N. Zhi, Degui Sun, Dianjianyi Zhang, Tao Li, Shengxu Chen, Wei Srinivasan, Sathanur R. Tiwari, Hemant K. Absher, Devin Ordovas, Jose M. Berenson, Gerald S. Arnett, Donna K. Irvin, Marguerite R. TI Association of DNA Methylation at CPT1A Locus with Metabolic Syndrome in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) Study SO PLOS ONE LA English DT Article ID EPIGENOME-WIDE ASSOCIATION; INSULIN-RESISTANCE; CARNITINE; EPIGENETICS; CHROMATIN; TRAITS; GENES; RISK AB In this study, we conducted an epigenome-wide association study of metabolic syndrome (MetS) among 846 participants of European descent in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN). DNA was isolated from CD4+ T cells and methylation at similar to 470,000 cytosine-phosphate-guanine dinucleotide (CpG) pairs was assayed using the Illumina Infinium HumanMethylation450 BeadChip. We modeled the percentage methylation at individual CpGs as a function of MetS using linear mixed models. A Bonferroni-corrected P-value of 1.1 x 10(-7) was considered significant. Methylation at two CpG sites in CPT1A on chromosome 11 was significantly associated with MetS (P for cg00574958 = 2.6x10(-14) and P for cg17058475 = 1.2x10(-9)). Significant associations were replicated in both European and African ancestry participants of the Bogalusa Heart Study. Our findings suggest that methylation in CPT1A is a promising epigenetic marker for MetS risk which could become useful as a treatment target in the future. C1 [Das, Mithun; Sha, Jin; Hidalgo, Bertha; Aslibekyan, Stella; Do, Anh N.; Irvin, Marguerite R.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA. [Zhi, Degui; Tiwari, Hemant K.] Univ Alabama Birmingham, Dept Biostat, Sect Stat Genet, Sch Publ Hlth, Birmingham, AL 35294 USA. [Sun, Dianjianyi; Zhang, Tao; Li, Shengxu; Chen, Wei; Srinivasan, Sathanur R.; Berenson, Gerald S.] Tulane Univ, Dept Epidemiol, Sch Publ Hlth & Trop Med, New Orleans, LA 70118 USA. [Absher, Devin] HudsonAlpha Inst Biotechnol, Huntsville, AL USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] IMDEA Food, Madrid, Spain. [Arnett, Donna K.] Univ Kentucky, Deans Off, Coll Publ Hlth, Lexington, KY USA. RP Irvin, MR (reprint author), Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA. EM irvinr@uab.edu FU US National Institutes of Health (NIH)/National Heart, Lung and Blood Institutes [R01HL104135, U01HL72524]; National Institute on Aging [R01AG016592]; National Institute of Environmental Health Science [R01ES021724] FX The GOLDN study (DKA) was funded by the US National Institutes of Health (NIH)/National Heart, Lung and Blood Institutes (http://www.nhlbi.nih.gov) grants R01HL104135 and U01HL72524. The Bogalusa Heart Study (GSB) was supported by grants R01AG016592 from the National Institute on Aging (https://www.nia.nih.gov) and R01ES021724 from National Institute of Environmental Health Science (http://www.niehs.nih.gov). No individuals employed or contracted by the funders played any role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 3 Z9 3 U1 3 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 25 PY 2016 VL 11 IS 1 AR e0145789 DI 10.1371/journal.pone.0145789 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9FZ UT WOS:000369527800011 PM 26808626 ER PT J AU Glenn, AE Davis, CB Gao, ML Gold, SE Mitchell, TR Proctor, RH Stewart, JE Snook, ME AF Glenn, Anthony E. Davis, C. Britton Gao, Minglu Gold, Scott E. Mitchell, Trevor R. Proctor, Robert H. Stewart, Jane E. Snook, Maurice E. TI Two Horizontally Transferred Xenobiotic Resistance Gene Clusters Associated with Detoxification of Benzoxazolinones by Fusarium Species SO PLOS ONE LA English DT Article ID ASPERGILLUS-KAWACHII; FUMONISIN PRODUCTION; FUNGAL ENDOPHYTES; MAIZE SEEDLINGS; BETA-LACTAMASES; VERTICILLIOIDES; PHYTOALEXINS; ECOLOGY; CORN; BIOTRANSFORMATION AB Microbes encounter a broad spectrum of antimicrobial compounds in their environments and often possess metabolic strategies to detoxify such xenobiotics. We have previously shown that Fusarium verticillioides, a fungal pathogen of maize known for its production of fumonisin mycotoxins, possesses two unlinked loci, FDB1 and FDB2, necessary for detoxification of antimicrobial compounds produced by maize, including the gamma-lactam 2-benzoxazolinone ( BOA). In support of these earlier studies, microarray analysis of F. verticillioides exposed to BOA identified the induction of multiple genes at FDB1 and FDB2, indicating the loci consist of gene clusters. One of the FDB1 cluster genes encoded a protein having domain homology to the metallo-beta-lactamase (MBL) superfamily. Deletion of this gene ( MBL1) rendered F. verticillioides incapable of metabolizing BOA and thus unable to grow on BOA-amended media. Deletion of other FDB1 cluster genes, in particular AMD1 and DLH1, did not affect BOA degradation. Phylogenetic analyses and topology testing of the FDB1 and FDB2 cluster genes suggested two horizontal transfer events among fungi, one being transfer of FDB1 from Fusarium to Colletotrichum, and the second being transfer of the FDB2 cluster from Fusarium to Aspergillus. Together, the results suggest that plant-derived xenobiotics have exerted evolutionary pressure on these fungi, leading to horizontal transfer of genes that enhance fitness or virulence. C1 [Glenn, Anthony E.; Davis, C. Britton; Gold, Scott E.; Mitchell, Trevor R.; Snook, Maurice E.] USDA, ARS, Richard B Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA USA. [Gao, Minglu] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Proctor, Robert H.] USDA, ARS, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol Res Unit, Peoria, IL USA. [Stewart, Jane E.] Colorado State Univ, Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Davis, C. Britton] Univ Georgia, Ctr Trop & Emerging Global Dis, Athens, GA 30602 USA. RP Glenn, AE (reprint author), USDA, ARS, Richard B Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA USA. EM anthony.glenn@ars.usda.gov NR 46 TC 0 Z9 0 U1 7 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 25 PY 2016 VL 11 IS 1 AR e0147486 DI 10.1371/journal.pone.0147486 PG 29 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9FZ UT WOS:000369527800108 PM 26808652 ER PT J AU Munoz-Gutierrez, JF Pierle, SA Schneider, DA Baszler, TV Stanton, JB AF Munoz-Gutierrez, Juan F. Pierle, Sebastian Aguilar Schneider, David A. Baszler, Timothy V. Stanton, James B. TI Transcriptomic Determinants of Scrapie Prion Propagation in Cultured Ovine Microglia SO PLOS ONE LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; NATURAL SCRAPIE; DENDRITIC CELLS; SELENOPROTEIN P; LYMPHOID-TISSUE; NEURONAL CELLS; MESSENGER-RNA; MOUSE MODELS; EXPRESSION; PROTEIN AB Susceptibility to infection by prions is highly dependent on the amino acid sequence and host expression of the cellular prion protein (PrPC); however, cellular expression of a genetically susceptible PrPC is insufficient. As an example, it has been shown in cultured cells that permissive and resistant sublines derived from the same parental population often have similar expression levels of PrPC. Thus, additional cellular factors must influence susceptibility to prion infection. The aim of this study was to elucidate the factors associated with relative permissiveness and resistance to scrapie prions in cultured cells derived from a naturally affected species. Two closely related ovine microglia clones with different prion susceptibility, but no detectable differences in PrPC expression levels, were inoculated with either scrapie-positive or scrapie-negative sheep brainstem homogenates. Five passages post-inoculation, the transcriptional profiles of mock and infected clones were sequenced using Illumina technology. Comparative transcriptional analyses identified twenty-two differentially transcribed genes, most of which were upregulated in poorly permissive microglia. This included genes encoding for selenoprotein P, endolysosomal proteases, and proteins involved in extracellular matrix remodeling. Furthermore, in highly permissive microglia, transforming growth factor beta-induced, retinoic acid receptor response 1, and phosphoserine aminotranspherase 1 gene transcripts were upregulated. Gene Set Enrichment Analysis identified proteolysis, translation, and mitosis as the most affected pathways and supported the upregulation trend of several genes encoding for intracellular proteases and ribosomal proteins in poorly permissive microglia. This study identifies new genes potentially involved in scrapie prion propagation, corroborates results from other studies, and extends those results into another cell culture model. C1 [Munoz-Gutierrez, Juan F.; Pierle, Sebastian Aguilar; Schneider, David A.; Baszler, Timothy V.] Washington State Univ, Coll Vet Med, Dept Pathol & Microbiol, Pullman, WA 99164 USA. [Schneider, David A.] USDA, Agr Res Serv, Pullman, WA USA. [Stanton, James B.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. [Munoz-Gutierrez, Juan F.] Univ Wyoming, Dept Vet Sci, Laramie, WY USA. [Pierle, Sebastian Aguilar] Inst Pasteur, Unite Plasticite Genome Bacterien, Dept Genomes & Genet, Paris, France. RP Stanton, JB (reprint author), Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA.; Munoz-Gutierrez, JF (reprint author), Univ Wyoming, Dept Vet Sci, Laramie, WY USA. EM juan.munoz@uwyo.edu; jbs@uga.edu RI Stanton, James/A-5277-2011 OI Stanton, James/0000-0002-7661-2631 FU United States Department of Agriculture, Agricultural Research Service [5348-32000-030-00D] FX This work was supported by the United States Department of Agriculture, Agricultural Research Service (5348-32000-030-00D). NR 63 TC 0 Z9 0 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 25 PY 2016 VL 11 IS 1 AR e0147727 DI 10.1371/journal.pone.0147727 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9FZ UT WOS:000369527800198 PM 26807844 ER PT J AU Trijatmiko, KR Duenas, C Tsakirpaloglou, N Torrizo, L Arines, FM Adeva, C Balindong, J Oliva, N Sapasap, MV Borrero, J Rey, J Francisco, P Nelson, A Nakanishi, H Lombi, E Tako, E Glahn, RP Stangoulis, J Chadha-Mohanty, P Johnson, AAT Tohme, J Barry, G Slamet-Loedin, IH AF Trijatmiko, Kurniawan R. Duenas, Conrado Tsakirpaloglou, Nikolaos Torrizo, Lina Arines, Felichi Mae Adeva, Cheryl Balindong, Jeanette Oliva, Norman Sapasap, Maria V. Borrero, Jaime Rey, Jessica Francisco, Perigio Nelson, Andy Nakanishi, Hiromi Lombi, Enzo Tako, Elad Glahn, Raymond P. Stangoulis, James Chadha-Mohanty, Prabhjit Johnson, Alexander A. T. Tohme, Joe Barry, Gerard Slamet-Loedin, Inez H. TI Biofortified indica rice attains iron and zinc nutrition dietary targets in the field SO SCIENTIFIC REPORTS LA English DT Article ID SOYBEAN FERRITIN GENE; STAPLE FOOD CROPS; NICOTIANAMINE-SYNTHASE; TRANSGENIC RICE; PLANTS; DIGESTION; GRAIN; SEED; FORTIFICATION; MALNUTRITION AB More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 mu g g(-1) iron (Fe) and 16 mu g g(-1) zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 mu g g(-1) Fe and 28 mu g g(-1) Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 mu g g(-1) in glasshouse settings; in contrast, under field conditions, 4 mu g g(-1) was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 mu g g(-1) Fe and 45.7 mu g g(-1) Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds. C1 [Trijatmiko, Kurniawan R.; Duenas, Conrado; Tsakirpaloglou, Nikolaos; Torrizo, Lina; Arines, Felichi Mae; Adeva, Cheryl; Balindong, Jeanette; Oliva, Norman; Sapasap, Maria V.; Rey, Jessica; Francisco, Perigio; Chadha-Mohanty, Prabhjit; Barry, Gerard; Slamet-Loedin, Inez H.] Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, DAPO Box 7777, Manila, Philippines. [Borrero, Jaime; Tohme, Joe] Ctr Int Agr Trop, Apartado Aereo 6713, Cali, Colombia. [Nelson, Andy] Int Rice Res Inst, Social Sci Div, DAPO Box 7777, Manila, Philippines. [Nelson, Andy] Univ Twente, Fac Geoinformat & Earth Observat ITC, POB 217, NL-7500 AE Enschede, Netherlands. [Nakanishi, Hiromi] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Global Agr Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan. [Lombi, Enzo] Univ S Australia, Ctr Environm Risk Assessment & Remediat, Adelaide, SA 5001, Australia. [Tako, Elad; Glahn, Raymond P.] Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, New York, NY 10021 USA. [Stangoulis, James] Flinders Univ South Australia, Sch Biol Sci, Adelaide, SA, Australia. [Johnson, Alexander A. T.] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia. [Trijatmiko, Kurniawan R.] Indonesian Ctr Agr Biotechnol & Genet Resources R, Bogor 16111, Indonesia. [Slamet-Loedin, Inez H.] Indonesian Inst Sci, Res Ctr Biotechnol, Cibinong 16911, Indonesia. RP Slamet-Loedin, IH (reprint author), Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, DAPO Box 7777, Manila, Philippines.; Slamet-Loedin, IH (reprint author), Indonesian Inst Sci, Res Ctr Biotechnol, Cibinong 16911, Indonesia. EM i.slamet-loedin@irri.org RI Nelson, Andrew/G-3649-2012; Lombi, Enzo/F-3860-2013; OI Nelson, Andrew/0000-0002-7249-3778; Lombi, Enzo/0000-0003-3384-0375; Tsakirpaloglou, Nikolaos/0000-0001-6710-0709; JOHNSON, ALEXANDER/0000-0002-1209-6447 FU HarvestPlus; Global Rice Science Partnership (GRiSP); U.S. Agency for International Development (USAID-Development of Rice Biotechnology Products for Asia grant) FX We thank M. Manzanilla, G. Borja, and P.B. Malabanan for technical assistance; F. Goto for providing the soybean ferritin polyclonal antibody; Lily Molina for ICP analysis at IRRI; Violeta Bartolome for statistical analysis; T. Kretzmar, A. Kohli and A. Macovei for reviewing the manuscript; and B. Hardy for editing the manuscript. This work was made possible through support provided by the grant from the HarvestPlus. Complementary funding from Global Rice Science Partnership (GRiSP) and the U.S. Agency for International Development (USAID-Development of Rice Biotechnology Products for Asia grant) is acknowledged. The contents of this manuscript are solely responsibility of the authors. NR 54 TC 8 Z9 9 U1 6 U2 39 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JAN 25 PY 2016 VL 6 AR 19792 DI 10.1038/srep19792 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB8TD UT WOS:000368788600001 PM 26806528 ER PT J AU Zhao, XB Zhu, JY AF Zhao, Xuebing Zhu, J. Y. TI Efficient Conversion of Lignin to Electricity Using a Novel Direct Biomass Fuel Cell Mediated by Polyoxometalates at Low Temperatures SO CHEMSUSCHEM LA English DT Article DE direct biomass fuel cells; lignins; phosphomolybdic acids; polyoxometalates; power density ID HIGH-TITER; PERFORMANCE; DELIGNIFICATION; CONSEQUENCES; OXIDATION; CLUSTERS; BIOCHAR; PHENOL; ENERGY; OXYGEN AB A novel polyoxometalates (POMs) mediated direct biomass fuel cell (DBFC) was used in this study to directly convert lignin to electricity at low temperatures with high power output and Faradaic efficiency. When phosphomolybdic acid H3PMo12O40 (PMo12) was used as the electron and proton carrier in the anode solution with a carbon electrode, and O-2 was directly used as the final electron acceptor under the catalysis of Pt, the peak power density reached 0.96mWcm(-2), 560times higher than that of phenol-fueled microbial fuel cells (MFCs). When the cathode reaction was catalyzed by PMo12, the power density could be greatly enhanced to 5mWcm(-2). Continuous operation demonstrated that this novel fuel cell was promising as a stable electrochemical power source. Structure analysis of the lignin indicated that the hydroxyl group content was reduced whereas the carbonyl group content increased. Both condensation and depolymerization takes place during the PMo12 oxidation of lignin. C1 [Zhao, Xuebing] Tsinghua Univ, Dept Chem Engn, Beijing 100084, Peoples R China. [Zhao, Xuebing; Zhu, J. Y.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI USA. [Zhao, Xuebing; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI USA. RP Zhu, JY (reprint author), Univ Wisconsin, Dept Biol Syst Engn, Madison, WI USA.; Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI USA. EM jzhu@fs.fed.us NR 38 TC 1 Z9 1 U1 27 U2 65 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1864-5631 EI 1864-564X J9 CHEMSUSCHEM JI ChemSusChem PD JAN 21 PY 2016 VL 9 IS 2 BP 197 EP 207 DI 10.1002/cssc.201501446 PG 11 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY SC Chemistry; Science & Technology - Other Topics GA DB9GU UT WOS:000368825200007 PM 26692572 ER PT J AU Zurek, K Nayduch, D AF Zurek, Klara Nayduch, Dana TI Bacterial Associations Across House Fly Life History: Evidence for Transstadial Carriage From Managed Manure SO JOURNAL OF INSECT SCIENCE LA English DT Article DE bacteria; larvae; pupae; puparia; adult ID MUSCA-DOMESTICA DIPTERA; ESCHERICHIA-COLI; MUSCIDAE; DIVERSITY; LARVAE; METAMORPHOSIS; PERSISTENCE; PREVALENCE; RETENTION; GUT AB House flies (Diptera: Muscidae; Musca domestica L.) associate with microbe-rich substrates throughout life history. Because larvae utilize bacteria as a food source, most taxa present in the larval substrate, e.g., manure, are digested or degraded. However, some species survive and are present as third-instar larvae begin pupation. During metamorphosis, many bacteria are again lost during histolysis of the larval gut and subsequent remodeling to produce the gut of the imago. It has been previously demonstrated that some bacterial species survive metamorphosis, being left behind in the puparium, present on the body surface, or in the gut of the emerged adult. We used a combined culture-molecular approach to identify viable microbes from managed manure residue and a wild population of house fly larvae, pupae, puparia, and adults to assess transstadial carriage. All larval (10/10), pupal (10/10), and puparial (10/10) cultures were positive for bacteria. Several bacterial species that were present in larvae also were present either in pupae or puparia. Four viable bacterial species were detectable in 6 of 10 imagoes reared from manure. Of note is the apparent transstadial carriage of Bacillus sonorensis, which has been associated with milk spoilage at dairies, and Alcaligenes faecalis, which can harbor numerous antibiotic resistance genes on farms. The potential of newly emerged flies to harbor and disseminate bacteria from managed manure on farms is an understudied risk that deserves further evaluation. C1 [Zurek, Klara] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Zurek, Klara; Nayduch, Dana] USDA ARS, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. RP Nayduch, D (reprint author), USDA ARS, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM kzurek@ksu.edu; dana.nayduch@ars.usda.gov FU USDA-ARS [3020-32000-007, 104] FX We thank K. Hochard for assistance with sample collection and processing. This work was supported by USDA-ARS National Program 104, Project number 3020-32000-007. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 22 TC 3 Z9 4 U1 4 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JAN 21 PY 2016 VL 16 DI 10.1093/jisesa/iev156 PG 4 WC Entomology SC Entomology GA DB4BP UT WOS:000368458000003 ER PT J AU Choe, E Drnevich, J Williams, MM AF Choe, Eunsoo Drnevich, Jenny Williams, Martin M., II TI Identification of Crowding Stress Tolerance Co-Expression Networks Involved in Sweet Corn Yield SO PLOS ONE LA English DT Article ID MAIZE SEEDLING TRANSCRIPTOME; ZEA-MAYS L.; GENE-EXPRESSION; PLANT-DENSITY; DROUGHT TOLERANCE; CYANOBACTERIAL FLAVODOXIN; TRANSGENIC TOBACCO; POLYAMINE RESEARCH; MICROARRAY DATA; DYNAMIC CHANGES AB Tolerance to crowding stress has played a crucial role in improving agronomic productivity in field corn; however, commercial sweet corn hybrids vary greatly in crowding stress tolerance. The objectives were to 1) explore transcriptional changes among sweet corn hybrids with differential yield under crowding stress, 2) identify relationships between phenotypic responses and gene expression patterns, and 3) identify groups of genes associated with yield and crowding stress tolerance. Under conditions of crowding stress, three high-yielding and three low-yielding sweet corn hybrids were grouped for transcriptional and phenotypic analyses. Transcriptional analyses identified from 372 to 859 common differentially expressed genes (DEGs) for each hybrid. Large gene expression pattern variation among hybrids and only 26 common DEGs across all hybrid comparisons were identified, suggesting each hybrid has a unique response to crowding stress. Over-represented biological functions of DEGs also differed among hybrids. Strong correlation was observed between: 1) modules with up-regulation in high-yielding hybrids and yield traits, and 2) modules with up-regulation in low-yielding hybrids and plant/ear traits. Modules linked with yield traits may be important crowding stress response mechanisms influencing crop yield. Functional analysis of the modules and common DEGs identified candidate crowding stress tolerant processes in photosynthesis, glycolysis, cell wall, carbohydrate/nitrogen metabolic process, chromatin, and transcription regulation. Moreover, these biological functions were greatly inter-connected, indicating the importance of improving the mechanisms as a network. C1 [Choe, Eunsoo; Williams, Martin M., II] USDA ARS, Global Change & Photosynthesis Res Unit, Urbana, IL USA. [Drnevich, Jenny] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. RP Williams, MM (reprint author), USDA ARS, Global Change & Photosynthesis Res Unit, Urbana, IL USA. EM martin.williams@ars.usda.gov NR 87 TC 0 Z9 0 U1 4 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 21 PY 2016 VL 11 IS 1 AR e0147418 DI 10.1371/journal.pone.0147418 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB5CE UT WOS:000368530300032 PM 26796516 ER PT J AU Grace, JB Anderson, TM Seabloom, EW Borer, ET Adler, PB Harpole, WS Hautier, Y Hillebrand, H Lind, EM Partel, M Bakker, JD Buckley, YM Crawley, MJ Damschen, EI Davies, KF Fay, PA Firn, J Gruner, DS Hector, A Knops, JMH MacDougall, AS Melbourne, BA Morgan, JW Orrock, JL Prober, SM Smith, MD AF Grace, James B. Anderson, T. Michael Seabloom, Eric W. Borer, Elizabeth T. Adler, Peter B. Harpole, W. Stanley Hautier, Yann Hillebrand, Helmut Lind, Eric M. Paertel, Meelis Bakker, Jonathan D. Buckley, Yvonne M. Crawley, Michael J. Damschen, Ellen I. Davies, Kendi F. Fay, Philip A. Firn, Jennifer Gruner, Daniel S. Hector, Andy Knops, Johannes M. H. MacDougall, Andrew S. Melbourne, Brett A. Morgan, John W. Orrock, John L. Prober, Suzanne M. Smith, Melinda D. TI Integrative modelling reveals mechanisms linking productivity and plant species richness SO NATURE LA English DT Article ID STRUCTURAL EQUATION MODELS; NET PRIMARY PRODUCTION; ECOSYSTEM FUNCTION; BIODIVERSITY; DIVERSITY; ECOLOGY; SCALE; HYPOTHESIS; VEGETATION; SIZE AB How ecosystem productivity and species richness are interrelated is one of the most debated subjects in the history of ecology(1). Decades of intensive study have yet to discern the actual mechanisms behind observed global patterns(2,3). Here, by integrating the predictions from multiple theories into a single model and using data from 1,126 grassland plots spanning five continents, we detect the clear signals of numerous underlying mechanisms linking productivity and richness. We find that an integrative model has substantially higher explanatory power than traditional bivariate analyses. In addition, the specific results unveil several surprising findings that conflict with classical models(4-7). These include the isolation of a strong and consistent enhancement of productivity by richness, an effect in striking contrast with superficial data patterns. Also revealed is a consistent importance of competition across the full range of productivity values, in direct conflict with some (but not all) proposed models. The promotion of local richness by macroecological gradients in climatic favourability, generally seen as a competing hypothesis(8), is also found to be important in our analysis. The results demonstrate that an integrative modelling approach leads to a major advance in our ability to discern the underlying processes operating in ecological systems. C1 [Grace, James B.] US Geol Survey, Wetland & Aquat Res Ctr, Lafayette, LA 70506 USA. [Anderson, T. Michael] Wake Forest Univ, Dept Biol, Winston Salem, NC 27109 USA. [Seabloom, Eric W.; Borer, Elizabeth T.; Lind, Eric M.] Univ Minnesota, Ecol Evolut & Behav, St Paul, MN 55108 USA. [Adler, Peter B.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Adler, Peter B.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Harpole, W. Stanley] UFZ Helmholtz Ctr Environm Res, Dept Physiol Divers, D-04318 Leipzig, Germany. [Harpole, W. Stanley] German Ctr Integrat Biodivers Res iDiv, D-04103 Leipzig, Germany. [Harpole, W. Stanley] Univ Halle Wittenberg, D-06108 Halle, Saale, Germany. [Hautier, Yann] Univ Utrecht, Dept Biol, Ecol & Biodivers Grp, NL-3584 CH Utrecht, Netherlands. [Hillebrand, Helmut] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol, Marine Environm, D-26381 Wilhelmshaven, Germany. [Paertel, Meelis] Univ Tartu, Inst Ecol & Earth Sci, EE-51005 Tartu, Estonia. [Bakker, Jonathan D.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Buckley, Yvonne M.] Univ Dublin Trinity Coll, Sch Nat Sci, Zool, Dublin 2, Ireland. [Crawley, Michael J.] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, Ascot SL5 7PY, Berks, England. [Damschen, Ellen I.; Orrock, John L.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Davies, Kendi F.; Melbourne, Brett A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Fay, Philip A.] ARS, Grassland Soil & Water Res Lab, USDA, Temple, TX 76502 USA. [Firn, Jennifer] Queensland Univ Technol, Sch Earth Environm & Biol Sci, Brisbane, Qld 4001, Australia. [Gruner, Daniel S.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Hector, Andy] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England. [Knops, Johannes M. H.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [MacDougall, Andrew S.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Morgan, John W.] La Trobe Univ, Dept Ecol Environm & Evolut, Bundoora, Vic 3083, Australia. [Prober, Suzanne M.] CSIRO Land & Water, Wembley, WA 6913, Australia. [Smith, Melinda D.] Colorado State Univ, Dept Biol, Ft Collins, CO 80526 USA. RP Grace, JB (reprint author), US Geol Survey, Wetland & Aquat Res Ctr, 700 Cajundome Blvd, Lafayette, LA 70506 USA. EM gracej@usgs.gov RI Gruner, Daniel/A-5166-2010; Partel, Meelis/D-5493-2012; Hillebrand, Helmut/I-1717-2014; Smith, Melinda/J-8987-2014; Prober, Suzanne/G-6465-2010; MacDougall, Andrew/F-2037-2011; Hector, Andrew/H-4199-2011; Hautier, Yann/D-5426-2015 OI Borer, Elizabeth/0000-0003-2259-5853; Gruner, Daniel/0000-0002-3153-4297; Partel, Meelis/0000-0002-5874-0138; Hillebrand, Helmut/0000-0001-7449-1613; Seabloom, Eric/0000-0001-6780-9259; Lind, Eric/0000-0003-3051-7724; Hector, Andrew/0000-0002-1309-7716; Hautier, Yann/0000-0003-4347-7741 FU US Geological Survey; Nutrient Network; National Science Foundation (NSF) Research Coordination Network [NSF-DEB-1042132]; National Science Foundation (NSF) Long Term Ecological Research program [NSF-DEB-1234162]; UMN Institute on the Environment [DG-0001-13] FX J.B.G. was supported by the US Geological Survey Ecosystems and Climate and Land use Change Programs. This work uses data from the Nutrient Network (http://nutnet.org) experiment, funded at the site scale by individual researchers. Coordination and data management were supported by funding to E.T.B. and E.W.S. from the National Science Foundation (NSF) Research Coordination Network (NSF-DEB-1042132) and Long Term Ecological Research (NSF-DEB-1234162 to Cedar Creek LTER) programs and the UMN Institute on the Environment (DG-0001-13). The Minnesota Supercomputer Institute hosts project data. The use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. Support for site-level activities is acknowledged in the Supplementary Information. We thank D. Laughlin for comments on the manuscript. NR 45 TC 38 Z9 38 U1 63 U2 179 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD JAN 21 PY 2016 VL 529 IS 7586 BP 390 EP + DI 10.1038/nature16524 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB2QT UT WOS:000368354800045 PM 26760203 ER PT J AU Girouard, NM Xu, SH Schueneman, GT Shofner, ML Meredith, JC AF Girouard, Natalie M. Xu, Shanhong Schueneman, Gregory T. Shofner, Meisha L. Meredith, J. Carson TI Site-Selective Modification of Cellulose Nanocrystals with Isophorone Diisocyanate and Formation of Polyurethane-CNC Composites SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE cellulose nanocrystal; nanoparticle modification; isocyanate chemistry; thermal stability; polyurethane; polymer nanocomposite; dispersion; mechanical properties ID NUCLEAR-MAGNETIC-RESONANCE; DRYING METHOD; NANOCOMPOSITES; CRYSTALLINITY; WHISKERS; NANOCELLULOSE; PERFORMANCE; NANOFIBERS; EXTRUSION; EMULSIONS AB The unequal reactivity of the two isocyanate groups in an isophorone diisocyante (IPDI) monomer was exploited to yield modified cellulose nanocrystals (CNCs) with both urethane and isocyanate functionality. The chemical functionality of the modified CNCs was verified with ATR-FTIR analysis and elemental analysis. The selectivity for the secondary isocyanate group using dibutyl tin dilaurate (DBTDL) as the reaction catalyst was confirmed with C-13 NMR. The modified CNCs showed improvements in the onset of thermal degradation by 35 degrees C compared to the unmodified CNCs. Polyurethane composites based on IPDI and a trifunctional polyether alcohol were synthesized using unmodified (um-CNC) and modified CNCs (m-CNC). The degree of nanoparticle dispersion was qualitatively assessed with polarized optical microscopy. It was found that the modification step facilitated superior nanoparticle dispersion compared to the um-CNCs, which resulted in increases in the tensile strength and work of fracture of over 200% compared to the neat matrix without degradation of elongation at break. C1 [Girouard, Natalie M.; Meredith, J. Carson] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA. [Shofner, Meisha L.] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. [Shofner, Meisha L.; Meredith, J. Carson] Georgia Inst Technol, Renewable Bioprod Inst, Atlanta, GA 30332 USA. [Xu, Shanhong] NIST, Gaithersburg, MD 20899 USA. [Schueneman, Gregory T.] US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. RP Meredith, JC (reprint author), Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA. EM carson.meredith@chbe.gatech.edu RI Meredith, Carson/B-3323-2009 OI Meredith, Carson/0000-0003-2519-5003 FU USDA Forest Products Laboratory (FPL) FX The authors of this paper would like to thank the USDA Forest Products Laboratory (FPL) for their financial support and collaboration. The authors thank J. Leisen of Georgia Inst. of Technology for the NMR measurements and discussions, Prof. E. Mintz of Clark Atlanta Univ. for useful discussions, D. Sherman of DS imaging West Lafayette, IN, for the TEM imaging of the CNCs, and R. Reiner and A. Rudie of the Forest Products Laboratory, Madison, WI, for providing CNCs for this research. NR 49 TC 0 Z9 0 U1 25 U2 63 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD JAN 20 PY 2016 VL 8 IS 2 BP 1458 EP 1467 DI 10.1021/acsami.5b10723 PG 10 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA DB6UL UT WOS:000368651100046 PM 26713564 ER PT J AU Huesing, JE Andres, D Braverman, MP Burns, A Felsot, AS Harrigan, GG Hellmich, RL Reynolds, A Shelton, AM van Rijssen, WJ Morris, EJ Eloff, JN AF Huesing, Joseph E. Andres, David Braverman, Michael P. Burns, Andrea Felsot, Allan S. Harrigan, George G. Hellmich, Richard L. Reynolds, Alan Shelton, Anthony M. van Rijssen, Wilna Jansen Morris, E. Jane Eloff, Jacobus N. TI Global Adoption of Genetically Modified (GM) Crops: Challenges for the Public Sector SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE agricultural biotechnology; genetically modified crops; regulatory assessments ID WESTERN CORN-ROOTWORM; FRUGIPERDA LEPIDOPTERA-NOCTUIDAE; FIELD-EVOLVED RESISTANCE; HORIZONTAL GENE-TRANSFER; GREEN-REVOLUTION; PESTICIDE USE;