FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Matthews, KK O'Brien, DJ Whitley, NC Burke, JM Miller, JE Barczewski, RA AF Matthews, K. K. O'Brien, D. J. Whitley, N. C. Burke, J. M. Miller, J. E. Barczewski, R. A. TI Investigation of possible pumpkin seeds and ginger effects on gastrointestinal nematode infection indicators in meat goat kids and lambs SO SMALL RUMINANT RESEARCH LA English DT Article DE Internal parasites; Meat goats; Lambs; Pumpkin; Ginger ID ANTHELMINTIC RESISTANCE; SHEEP; FARMS AB In four experiments, 77 naturally-infected Boer crossbred kids and 28 artificially-inoculated Katandin lambs were used to evaluate the effect of pumpkin seeds (Exp 1; 21 kids), ginger or pumpkin seed drench (Exp 2; 30 kids) and pumpkin seed oil (Exp 3 and 4: 28 lambs and 26 kids, respectively) on gastrointestinal nematode (GIN) indicators. In all experiments, kids and lambs were placed in individual pens and received pre-weighed rations of a commercially pelleted meat goat or sheep diet daily. In Exp 1, kids were supplemented with ground pumpkin seeds (PUM; n=10) mixed into feed daily at a rate of 5 g/kg body weight (BW) or were not supplemented (CON; n=11) for 21 days. In Exp 2, kids were orally drenched with water (CON; n=10), 5 g pumpkin seed/kg BW (PUM; n=10) or 3 g ginger/kg BW (GIR; n=10) every other day for 42 days. In Exp 3, lambs were orally drenched with 2 ml/kg water (CON; n=7), 2.0 ml/kg BW pumpkin seed oil once every 7 days (PUM1; n=10), or 2.0 ml/kg BW pumpkin seed oil daily for 3 out of every 7 days (PUM2; n=11) for 28 days. In Exp 4, kids were orally drenched with 2 ml/kg water (CON; n=13), or 2.0 ml/kg BW pumpkin seed oil (PUM; n=13) every other day for 35 days. In all experiments, BW, daily feed intake and blood and fecal samples were collected every 7 days. All animals in Exp 2 were harvested at a USDA-inspected abattoir and abomasal and small intestinal contents were collected for total worm counts. The FEC were similar for treatments in all experiments. Treatment influenced PCV (P<0.05) only in Exp 1 and 4. In Exp 2, at harvest, there was a tendency (P = 0.08) for CON animals to have a higher number of total GIN than GIR-treated animals, but PUM-treated animals were intermediate. BW were similar for treatments in Exp 1, 2 and 3 while CON animals in Exp 4 had a greater BW than PUM-treated animals on day 7 only and were similar thereafter (treatment by day interaction, P<0.05). In these studies, pumpkin and ginger treatments administered were not effective in reducing FEC in meat goat kids or lambs. (C) 2016 Elsevier B.V. All rights reserved. C1 [Matthews, K. K.; O'Brien, D. J.; Barczewski, R. A.] Delaware State Univ, Dept Agr, 1200 N Dupont Hwy, Dover, DE 19901 USA. [Whitley, N. C.] Ft Valley State Univ, Cooperat Extens Program, Ft Valley, GA 31030 USA. [Burke, J. M.] ARS, Dale Bumpers Small Farms Res Ctr, USDA, Booneville, AR 72927 USA. [Miller, J. E.] Louisiana State Univ, Dept Pathobiol Sci, Sch Vet Med, Baton Rouge, LA 70803 USA. [Miller, J. E.] Louisiana State Univ, Dept Anim Sci, Baton Rouge, LA 70803 USA. [Miller, J. E.] Louisiana State Univ, Dept Vet Sci, Baton Rouge, LA 70803 USA. [O'Brien, D. J.] Virginia State Univ, Cooperat Extens, POB 9081, Petersburg, VA 23806 USA. [Matthews, K. K.] Tuskegee Univ, Dept Agr & Environm Sci, 205 Milbank Hall, Tuskegee, AL 36088 USA. RP O'Brien, DJ (reprint author), Delaware State Univ, Dept Agr, 1200 N Dupont Hwy, Dover, DE 19901 USA.; O'Brien, DJ (reprint author), Virginia State Univ, Cooperat Extens, POB 9081, Petersburg, VA 23806 USA. EM dobrien@vsu.edu FU Northeast Sustainable Agriculture Research and Education (NESARE) [LNE08-269] FX Funding was supported by a Northeast Sustainable Agriculture Research and Education (NESARE; LNE08-269) grant. The authors thank graduate students Elizabeth Crook and Jenna Warren and undergraduate students Jarvis Scott and Kevin Beaudoin as well as Delaware State University's College of Agriculture and Related Science Dean, Dyremple Marsh and farm technician, Lee Dulin, for support and assistance with this research project. NR 18 TC 2 Z9 2 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-4488 EI 1879-0941 J9 SMALL RUMINANT RES JI Small Ruminant Res. PD MAR PY 2016 VL 136 BP 1 EP 6 DI 10.1016/j.smallrumres.2015.12.036 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK0MH UT WOS:000374606400001 ER PT J AU Backoulou, GF Elliott, NC Giles, KL AF Backoulou, Georges F. Elliott, Norman C. Giles, Kristopher L. TI Using Multispectral Imagery to Compare the Spatial Pattern of Injury to Wheat Caused by Russian Wheat Aphid and Greenbug SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID STRESS; HOMOPTERA AB The Russian wheat aphid, Diuraphis noxia (Kurdjumov), and greenbug, Schizaphis graminum (Rondani) (Hemiptera: Aphididae), are important insect pests of wheat, Triticum aestivum L. Outbreaks of both pests in commercial wheat fields occur almost every year in the Great Plains of the United States. Infestations of both pests in wheat fields are clumped in distribution, and as a result so is the injury to plants in the field. The spatial pattern of patches of plants injured by the two aphid pests can be detected using airborne multispectral imaging. This study used multispectral imagery of wheat fields infested by the two species of aphids analyzed using spatial-pattern metrics with the purpose of determining differences between species in the spatial pattern of injury to wheat plants in fields. Analyses using Students t-tests revealed patches of wheat within fields injured by Russian wheat aphid were larger, more complex in shape, and more isolated than patches of wheat injured by greenbug. Our findings progress toward developing practical methods using multispectral imagery to identify wheat fields infested by aphid pests and to differentiate fields infested by Russian wheat aphid from greenbug. C1 [Backoulou, Georges F.; Elliott, Norman C.; Giles, Kristopher L.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Noble Res Ctr 127, Stillwater, OK 74078 USA. [Elliott, Norman C.] USDA ARS, 1301 N Western Rd, Stillwater, OK 74075 USA. RP Backoulou, GF (reprint author), Oklahoma State Univ, Dept Entomol & Plant Pathol, Noble Res Ctr 127, Stillwater, OK 74078 USA. NR 16 TC 0 Z9 0 U1 4 U2 7 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD MAR PY 2016 VL 41 IS 1 BP 1 EP 7 PG 7 WC Entomology SC Entomology GA DL7NK UT WOS:000375827200001 ER PT J AU Sa, SG Lima, DCV Silva, LTR Pinheiro, JW Dubey, JP Silva, JCR Mota, RA AF Sa, Silvio G. Lima, Debora C. V. Silva, Luana T. R. Pinheiro Junior, Jose W. Dubey, Jitender P. Silva, Jean C. R. Mota, Rinaldo A. TI Seroprevalence of Toxoplasma gondii among turkeys on family farms in the state of Northeastern Brazil SO ACTA PARASITOLOGICA LA English DT Article DE Toxoplasmosis; turkeys; chickens; family poultry ID FERNANDO-DE-NORONHA; WILD ANIMALS; ANTIBODY DETECTION; SEROLOGIC SURVEY; HIGH PREVALENCE; CHICKENS; INFECTION; HUMANS; EGYPT; PCR AB The aim of the present study was to investigate the prevalence of antibodies against Toxoplasma gondii in turkeys and chickens on family farms in the semi-arid region of the state of Pernambuco, Brazil. In order to do so, 204 sera samples from turkeys were analyzed using the Modified Agglutination Test (MAT) and 322 sera samples from chickens were assessed using the Immunofluorescence Assay (IFA). Twenty-eight properties contained turkeys and 23 of these properties also contained chickens. The relative frequency of positive turkeys was 11% (21/204), with 46.6% (13/28) of the properties containing at least one positive turkey. The frequency of positive chickens was 25.8% (83/322), with 95.6% (22/23) of the properties containing at least one positive chicken. Based on the results of the present study, it was possible to conclude that turkeys can serve as an indicator of environmental contamination by oocysts of T. gondii. However, they are less effective than chickens bred in the same conditions. The increasing demand from consumers for naturally produced products should worry local sanitary authorities due to the high prevalence of antibodies against T. gondii found in this type of rearing system, particularly among chickens. C1 [Sa, Silvio G.; Lima, Debora C. V.; Silva, Luana T. R.; Pinheiro Junior, Jose W.; Silva, Jean C. R.; Mota, Rinaldo A.] Univ Fed Rural Pernambuco, Dept Vet Med, Lab Doencas Infectocontagiosas Anim Domest, Rua Dom Manoel Medeiros,S-N Dois Irmaos, BR-52171900 Recife, PE, Brazil. [Dubey, Jitender P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. RP Mota, RA (reprint author), Univ Fed Rural Pernambuco, Dept Vet Med, Lab Doencas Infectocontagiosas Anim Domest, Rua Dom Manoel Medeiros,S-N Dois Irmaos, BR-52171900 Recife, PE, Brazil. EM rinaldo.mota@hotmail.com FU Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) FX The authors would like to thank the Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) for financial support. NR 33 TC 0 Z9 0 U1 1 U2 2 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 1230-2821 EI 1896-1851 J9 ACTA PARASITOL JI Acta Parasitolog. PD MAR PY 2016 VL 61 IS 2 BP 401 EP 405 DI 10.1515/ap-2016-0053 PG 5 WC Parasitology SC Parasitology GA DK5PP UT WOS:000374972400025 PM 27078666 ER PT J AU Korres, NE Norsworthy, JK Tehranchian, P Gitsopoulos, TK Loka, DA Oosterhuis, DM Gealy, DR Moss, SR Burgos, NR Miller, MR Palhano, M AF Korres, Nicholas E. Norsworthy, Jason K. Tehranchian, Parsa Gitsopoulos, Thomas K. Loka, Dimitra A. Oosterhuis, Derrick M. Gealy, David R. Moss, Stephen R. Burgos, Nilda R. Miller, M. Ryan Palhano, Matheus TI Cultivars to face climate change effects on crops and weeds: a review SO AGRONOMY FOR SUSTAINABLE DEVELOPMENT LA English DT Review DE Allelopathy; Stress tolerance; Climate change; Carbon dioxide; Drought; Temperature; Weed competition; Competitive ability; Cultivar selection; Integrated weed management ID ECHINOCHLOA-CRUS-GALLI; RICE ORYZA-SATIVA; ISOTOPE DISCRIMINATION ANALYSIS; ABUTILON-THEOPHRASTI GROWTH; ATMOSPHERIC CARBON-DIOXIDE; SOIL-WATER AVAILABILITY; AIR CO2 ENRICHMENT; CORN ZEA-MAYS; ELEVATED CO2; COMPETITIVE ABILITY AB Climate change is caused by the release of greenhouse gases in the atmosphere. Climate change will impact many activities, but its effects on agricultural production could be acute. Estimates of annual damages in agriculture due to temperature increase or extended periods of drought will be more costly than damages in other activities. Yield losses are caused both by direct effects of climate change on crops and by indirect effects such as increased inputs in crop production for weed control. One possible solution to counteract the effects of climate change is to seek crop cultivars that are adapted to highly variable, extreme climatic conditions and pest changes. Here we review the effects of climate change on crop cultivars and weeds. Biomass increase will augment marketable yield by 8-70 % for C3 cereals, by 20-144 % for cash and vegetable crops, and by 6-35 % for flowers. Such positive effects could however be reduced by decreasing water and nutrient availability. Rising temperature will decrease yields of temperature-sensitive crops such as maize, soybean, wheat, and cotton or specialty crops such as almonds, grapes, berries, citrus, or stone fruits. Rice, which is expected to yield better under increased CO2, will suffer serious yield losses under high temperatures. Drought stress should decrease the production of tomato, soybean, maize, and cotton. Nevertheless, reviews on C4 photosynthesis response to water stress in interaction with CO2 concentration reveal that elevated CO2 concentration lessens the deleterious effect of drought on plant productivity. C3 weeds respond more strongly than C4 types to CO2 increases through biomass and leaf area increases. The positive response of C3 crops to elevated CO2 may make C4 weeds less competitive for C3 crops, whereas C3 weeds in C4 or C3 crops could become a problem, particularly in tropical regions. Temperature increases will mainly affect the distribution of weeds, particularly C4 type, by expanding their geographical range. This will enhance further yield losses and will affect weed management systems negatively. In addition, the expansion of invasive weed species such as itchgrass, cogongrass, and witchweed facilitated by temperature increases will increase the cost for their control. Under water or nutrient shortage scenarios, an r-strategist with characteristics in the order S-C-R, such as Palmer amaranth, large crabgrass, johnsongrass, and spurges, will most probably prevail. Selection of cultivars that secure high yields under climate change but also by competing weeds is of major importance. Traits related with (a) increased root/shoot ratio, (b) vernalization periods, (c) maturity, (d) regulation of node formation and/or internode distance, (e) harvest index variations, and (f) allelopathy merit further investigation. The cumulative effects of selecting a suitable stress tolerator-competitor cultivar will be reflected in reductions of environmental pollution, lower production costs, and sustainable food production. C1 [Korres, Nicholas E.; Norsworthy, Jason K.; Tehranchian, Parsa; Oosterhuis, Derrick M.; Burgos, Nilda R.; Miller, M. Ryan; Palhano, Matheus] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Gitsopoulos, Thomas K.] Inst Plant Breeding & Phytogenet Resources, Hellen Agr Org Demeter, POB 60324, Thessaloniki, Greece. [Loka, Dimitra A.] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Ceredigion SY23 3EB, Dyfed, Wales. [Gealy, David R.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Moss, Stephen R.] Rothamsted Res, Harpenden AL5 2JQ, Herts, England. RP Korres, NE (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. EM nkorres@yahoo.co.uk NR 229 TC 1 Z9 1 U1 71 U2 125 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1774-0746 EI 1773-0155 J9 AGRON SUSTAIN DEV JI Agron. Sustain. Dev. PD MAR PY 2016 VL 36 IS 1 AR 12 DI 10.1007/s13593-016-0350-5 PG 22 WC Agronomy; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY SC Agriculture; Science & Technology - Other Topics GA DJ6NB UT WOS:000374329100012 ER PT J AU Halvorson, AD Stewart, CE Del Grosso, SJ AF Halvorson, Ardell D. Stewart, Catherine E. Del Grosso, Stephen J. TI Manure and Inorganic Nitrogen Affect Irrigated Corn Yields and Soil Properties SO AGRONOMY JOURNAL LA English DT Article ID NO-TILL CORN; OXIDE EMISSIONS; CATTLE MANURE; DAIRY MANURE; GRAIN-YIELD; COMPOST APPLICATIONS; ORGANIC-MATTER; STOVER REMOVAL; USE EFFICIENCY; FERTILIZER AB Manure can substitute for inorganic N fertilizers and can mitigate potential soil deterioration under irrigated corn (Zea mays L.) silage production, but the impact on yields, soil C and N have not been thoroughly studied in the semiarid western United States. Five N source treatments (dairy manure [DM, 412 kg N ha(-1)], DM + AgrotainPlus [DM+AP], SuperU [SU, 179 kg N ha(-1)], urea [179 kg N ha(-1)], and control [no N applied]) were studied (3 yr) to determine effects on silage and grain yields, N uptake, and changes in soil properties (soil organic carbon [SOC], total soil nitrogen [TSN], nitrate-nitrogen [NO3-N], and soil test phosphorus [STP]) in an irrigated, conventionally-tilled clay loam soil under continuous corn silage production. Silage, grain, and stover yields and N uptake were greater with N application than control, with no difference between DM, DM+AP, urea, and SU. Fall soil NO3-N was greater with N application than control (0-90-cm depth), but lower with DM than for SU (0-180-cm depth). Soil test P was seven times greater with DM than with inorganic P aft er 3 yr. The SOC and TSN were strongly related to root and DM inputs, increasing with DM application but decreasing or remaining constant for SU, urea, and control treatments. Dairy manure is a valuable source of C, N, and P for irrigated tilled corn silage production with minimal NO3-N leaching or excessive soil P after 3 yr and can ameliorate residue removal and tillage impacts. C1 [Halvorson, Ardell D.; Stewart, Catherine E.; Del Grosso, Stephen J.] USDA ARS, 2150 Ctr Ave,Bldg D,Ste 100, Ft Collins, CO 80526 USA. RP Stewart, CE (reprint author), USDA ARS, 2150 Ctr Ave,Bldg D,Ste 100, Ft Collins, CO 80526 USA. EM Catherine.Stewart@ars.usda.gov OI Stewart, Catherine/0000-0003-1216-0450 FU Agricultural Research Service under the ARS GRACEnet Project FX The authors thank B. Floyd, R. D'Adamo, T. Delorean, A. Brandt, and many student assistants for their assistance and analytical support in collecting, processing, and analyzing the soil and plant samples and data reported herein, the ARDEC Staff for help with plot maintenance, and Koch Agronomic Services (Wichita, KS) for providing the SuperU and AgrotainPlus used in the study. This publication is based on work supported by the Agricultural Research Service under the ARS GRACEnet Project. Trade names and company names are included for the benefit of the reader and do not imply any endorsement or preferential treatment of the product by the authors or the USDA-ARS. NR 54 TC 3 Z9 3 U1 12 U2 21 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2016 VL 108 IS 2 BP 519 EP 531 DI 10.2134/agronj2015.0402 PG 13 WC Agronomy SC Agriculture GA DL1XA UT WOS:000375425300005 ER PT J AU Thivierge, MN Jego, G Belanger, G Bertrand, A Tremblay, GF Rotz, CA Qian, BD AF Thivierge, Marie-Noelle Jego, Guillaume Belanger, Gilles Bertrand, Annick Tremblay, Gaetan F. Rotz, C. Alan Qian, Budong TI Predicted Yield and Nutritive Value of an Alfalfa-Timothy Mixture under Climate Change and Elevated Atmospheric Carbon Dioxide SO AGRONOMY JOURNAL LA English DT Article ID ENVIRONMENTAL-MODEL HADGEM1; PERENNIAL FORAGE CROPS; GROWING DEGREE-DAYS; TRIFOLIUM-REPENS L; AGRICULTURE IMPLICATIONS; INTEGRATED ASSESSMENT; PHYSICAL-PROPERTIES; SHORTGRASS STEPPE; NODULATED ALFALFA; SIMULATION-MODEL AB Climate change studies have oft en focused on individual forage species although legume-grass mixtures are predominant on dairy farms in northern areas of North America. We assessed the effect of (i) future climate conditions (temperature and precipitation) and elevated atmospheric CO2 concentration ([CO2]), separately and together, on yield of alfalfa (Medicago sativa L.) and timothy (Phleum pratense L.), grown alone or in mixture, and (ii) an adaptation strategy (timing and number of harvests) on future yield and nutritive value of an alfalfa-timothy mixture. Forage dry matter (DM) yield and nutritive value for two contrasting climate areas in eastern Canada were simulated with the Integrated Farm System Model over two future periods (2020-2049 and 2050-2079) using three climate models and two representative concentration pathways (RCP 4.5 and 8.5) of greenhouse gas emissions. Under projected future climate and without adaptation, annual forage yield of both species and the mixture increased in the colder area and decreased in the warmer area. In both areas, first-cut yield increased due to faster growing degree-day accumulation, while regrowth yield decreased due to greater water and temperature stresses. Under elevated [CO2], annual yield and the alfalfa percentage in the mixture increased. When combining climate change and elevated [CO2], yield increased, except with the more drastic scenario (RCP 8.5, 2050-2079) in the warmer area, and forage nutritive value was reduced. With adaptation, the mixture yield was increased from 5 to 35%, while nutritive value was generally maintained under all future scenarios, mostly because of additional cuts. C1 [Thivierge, Marie-Noelle; Jego, Guillaume; Belanger, Gilles; Bertrand, Annick; Tremblay, Gaetan F.] Agr & Agri Food Canada, Quebec Res & Dev Ctr, 2560 Hochelaga Blvd, Quebec City, PQ G1V 2J3, Canada. [Rotz, C. Alan] USDA, Pasture Syst & Watershed Management Res Unit, 3702 Curtin Rd, University Pk, PA 16802 USA. [Qian, Budong] Agr & Agri Food Canada, Ottawa Res & Dev Ctr, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada. RP Jego, G (reprint author), Agr & Agri Food Canada, Quebec Res & Dev Ctr, 2560 Hochelaga Blvd, Quebec City, PQ G1V 2J3, Canada. EM guillaume.jego@agr.gc.ca FU Dairy Research Cluster as part of the Canadian Agri-Science Clusters Initiative of Agriculture and Agri-Food Canada; Agriculture and Agri-Food Canada FX This study was funded by the Dairy Research Cluster as part of the Canadian Agri-Science Clusters Initiative of Agriculture and Agri-Food Canada. The senior author gratefully acknowledges the financial support from Agriculture and Agri-Food Canada through the Visiting Fellowships in Canadian Government Laboratories program. The authors thank Rene Morissette for his assistance in data processing and Francois Thibodeau for his thorough work with IFSM simulations. NR 76 TC 1 Z9 1 U1 15 U2 19 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2016 VL 108 IS 2 BP 585 EP 603 DI 10.2134/agronj2015.0484 PG 19 WC Agronomy SC Agriculture GA DL1XA UT WOS:000375425300012 ER PT J AU Yost, MA Kitchen, NR Sudduth, KA Sadler, EJ Baffaut, C Volkmann, MR Drummond, ST AF Yost, Matt A. Kitchen, Newell R. Sudduth, Kenneth A. Sadler, Edward J. Baffaut, Claire Volkmann, Matthew R. Drummond, Scott T. TI Long-Term Impacts of Cropping Systems and Landscape Positions on Claypan-Soil Grain Crop Production SO AGRONOMY JOURNAL LA English DT Article ID YIELD STABILITY; RIVER-BASIN; ELECTRICAL-CONDUCTIVITY; AGROECOSYSTEM RESEARCH; MANAGEMENT-SYSTEMS; ROTATION; WATER; TILLAGE; WHEAT; FERTILIZATION AB Sustainable grain crop production on vulnerable claypan soils requires improved knowledge of long-term impacts of conservation cropping systems (CS). Therefore, effects of CS and landscape positions (LP) on corn (Zea mays L.), soybean [Glycine max (L.) Merr.], and wheat (Triticum aestivum L.) production were evaluated from 1991 to 2010 on a claypan soil in Missouri. Replicated treatments were three CS (2-yr mulch till corn-soybean [MTCS], 2-yr no-till corn-soybean [NTCS], and 3-yr notill corn-soybean-wheat-cover crop [NTCSW]) and three LP (summit, backslope, and footslope). Corn yield was equivalent among CS on the summit, 13% higher for NTCS and NTCSW on the backslope, and 14% lower for NTCSW on the footslope. Soybean yield was 8% higher on the summit, 24% higher on the backslope in NTCS and NTCSW, and 12% higher on the footslope in NTCSW than MTCS. Corn yield was more stable in NTCS and NTCSW than MTCS and increased in stability from the footslope to summit. Soybean yield was less stable in NTCS and NTCSW than MTCS and LP effects on stability were similar to corn. The coefficient of variation (CV) of corn yield across years was 10% points lower in NTCS and on the footslope, and of soybean yield was 10% points lower at the footslope and summit. Wheat production was not affected by LP. Results indicate that conservation systems oft en can maintain grain crop productivity equal to, increase yield stability above, and reduce yield variability below those of a conventional system on claypan soils. C1 [Yost, Matt A.; Kitchen, Newell R.; Sudduth, Kenneth A.; Sadler, Edward J.; Baffaut, Claire; Volkmann, Matthew R.; Drummond, Scott T.] Univ Missouri, Cropping Syst & Water Qual Res Unit, USDA ARS, 269Agr Engn Bldg, Columbia, MO 65211 USA. RP Kitchen, NR (reprint author), Univ Missouri, Cropping Syst & Water Qual Res Unit, USDA ARS, 269Agr Engn Bldg, Columbia, MO 65211 USA. EM Newell.Kitchen@ars.usda.gov NR 52 TC 1 Z9 1 U1 5 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2016 VL 108 IS 2 BP 713 EP 725 DI 10.2134/agronj2015.0413 PG 13 WC Agronomy SC Agriculture GA DL1XA UT WOS:000375425300024 ER PT J AU Grabber, JH AF Grabber, John H. TI Prohexadione-Calcium Improves Stand Density and Yield of Alfalfa Interseeded into Silage Corn SO AGRONOMY JOURNAL LA English DT Article ID STRIP INTERCROPPING SYSTEM; GROWTH-RETARDANTS; SEED TREATMENT; COVER CROPS; DAIRY-COWS; COMPANION CROPS; FORAGE YIELD; DRY-MATTER; NITROGEN; ESTABLISHMENT AB Interseeded alfalfa (Medicago sativa L.) could serve as a dual-purpose crop to provide groundcover for silage corn (Zea mays L.) and forage during subsequent years of production, but interspecific competition oft en leads to poor stands of alfalfa and unsatisfactory yields of corn. Four experiments evaluated whether interspecific competition could be alleviated by application of plant growth retardants (PGRs) on alfalfa interseeded at corn planting. In three experiments, foliar application of prohexadione-calcium (PHD) on seedlings approximately doubled stand density of interseeded alfalfa during establishment in corn and following 1 yr of alfalfa forage production. Untimely PHD application in one experiment, however, failed to prevent stand failure of alfalfa growing under warm and damp conditions that favored vigorous growth of corn. When successfully established without PHD, average dry matter yield (DMY) of interseeded alfalfa the year following corn was 85% greater than conventionally spring-seeded alfalfa; prior year PHD application further increased DMY of interseeded alfalfa by 15%. When tested with PHD in two experiments, seed soaking or coating with uniconazole (UCZ) or paclobutrazol (PBZ) reduced stand density and had neutral or adverse impacts on DMY of alfalfa following corn. Interseeding reduced silage corn DMY by an average of 10% compared to monoculture corn and PGR treatment of alfalfa usually failed to alleviate yield depression in corn. Overall, PHD shows promise for enhancing the establishment and subsequent yield of interseeded alfalfa, but further work is needed to refine this production system for farm use. C1 [Grabber, John H.] USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. RP Grabber, JH (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. EM John.Grabber@ars.usda.gov NR 55 TC 0 Z9 0 U1 10 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2016 VL 108 IS 2 BP 726 EP 735 DI 10.2134/agronj2015.0466 PG 10 WC Agronomy SC Agriculture GA DL1XA UT WOS:000375425300025 ER PT J AU Baumhardt, RL Mauget, SA Schwartz, RC Jones, OR AF Baumhardt, R. L. Mauget, S. A. Schwartz, R. C. Jones, O. R. TI El Nino Southern Oscillation Effects on Dryland Crop Production in the Texas High Plains SO AGRONOMY JOURNAL LA English DT Article ID GRAIN-SORGHUM YIELD; WINTER-WHEAT; IRRIGATION; ENSO; NITROGEN; TILLAGE AB Risk averse dryland crop management in the U.S. southern High Plains stabilizes annual yields, however in some years the full yield potential is unrealized thereby reducing the overall cropping system productivity. Equatorial Pacific sea surface temperature anomalies (SSTA) systematically couple with the atmosphere to produce predictable El Nino-Southern Oscillation (ENSO) weather in much of North America that could be exploited for improved crop management. Our objective was to evaluate ENSO effects on site-specific growth and yield of wheat (Triticum aestivum L.) and grain sorghum [Sorghum bicolor (L.) Moench] in a dryland wheat-sorghum-fallow (WSF) rotation from 1954 to 2011. Measurements from contour farmed terraces were grouped by common ENSO phase years based on 3-mo classification ending in June (April-June, AMJ) or August (June-August, JJA) that permits timely management of sorghum or wheat. Compared with years classified during September to November (SON) or mature ENSO phases, the JJA classification identified 78 and 68% of common La Nifia and El Nino years, respectively, and may have potential application to wheat management. Poor agreement between SON and AMJ phase classifications reduced common La Nina and El Nino years to 61 and 53% respectively, and may limit grain sorghum management. Compared with La Nina, wheat growing-season precipitation increased 31% for El Nino and contributed to a 55% increase in detrended grain yield differences. For the Texas High Plains, the ENSO phase effects on sorghum grain yield were less pronounced than for wheat, suggesting ENSO based management is better suited to wheat. C1 [Baumhardt, R. L.; Schwartz, R. C.; Jones, O. R.] USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. [Mauget, S. A.] USDA ARS, Cropping Syst Res Lab, 3810 4th St, Lubbock, TX 79424 USA. RP Baumhardt, RL (reprint author), USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. EM r.louis.baumhardt@ars.usda.gov FU Ogallala Aquifer Program; USDA Agricultural Research Service; Kansas State University; Texas A&M AgriLife Research; Texas A&M AgriLife Extension Service; Texas Tech University; West Texas AM University FX This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 31 TC 1 Z9 1 U1 3 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2016 VL 108 IS 2 BP 736 EP 744 DI 10.2134/agronj2015.0403 PG 9 WC Agronomy SC Agriculture GA DL1XA UT WOS:000375425300026 ER PT J AU Friedman, JM Hunt, ER Mutters, RG AF Friedman, Jennifer M. Hunt, E. Raymond, Jr. Mutters, Randall G. TI Assessment of Leaf Color Chart Observations for Estimating Maize Chlorophyll Content by Analysis of Digital Photographs SO AGRONOMY JOURNAL LA English DT Article ID NITROGEN-USE EFFICIENCY; VISIBLE BAND INDEX; VEGETATION INDEXES; NARROW-BAND; BROAD-BAND; RICE; MANAGEMENT; CORN; YIELD; METER AB Developed as a nondestructive aid for estimating the N content in rice (Oryza sativa L.) crops, leaf color charts (LCCs) are a numbered series of plastic panels that range from yellow-green to dark green. By visual comparison, the panel value closest in color to a leaf indicates whether N is deficient, sufficient, or in excess. Because the selected values depend on subjective decisions by an observer, our goal was to determine whether spectral reflectances or digital color photographs could provide an objective, reproducible, and potentially automated method for determining LCC values. Maize (Zea mays L.) leaves were collected on two dates from an ongoing N fertilization experiment. Observed LCC panel values of selected leaves were highly correlated to chlorophyll content and chlorophyll meter values. Spectral reflectances and digital photographs were analyzed to predict the LCC panel value that was observed for each leaf. Supervised classifications of digital photographs using minimum distance provided reasonable predictions of the LCC value, but the spectral angle mapper did not. The dark green color index and the triangular greenness index predicted LCC panel values well. Uncorrected digital photographs (raw) produced better agreement with observed LCC panel values when using spectral indices, whereas color-corrected photographs (JPEG) produced better agreement using the supervised classification methods. We concluded that subjective visual observations using a LCC were not worse than the objective methods for estimating leaf chlorophyll content. Visual observations using LCCs may be an easy-to-use and low-cost method for managing N at smaller scales. C1 [Friedman, Jennifer M.; Hunt, E. Raymond, Jr.] USDA ARS, Beltsville Agr Res Ctr, Hydrol & Remote Sensing Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Mutters, Randall G.] Univ Calif Oroville, Agr & Nat Resources, Cooperat Extens Butte Cty, Oroville, CA 95965 USA. RP Hunt, ER (reprint author), USDA ARS, Beltsville Agr Res Ctr, Hydrol & Remote Sensing Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Raymond.Hunt@ars.usda.gov NR 54 TC 0 Z9 0 U1 5 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAR-APR PY 2016 VL 108 IS 2 BP 822 EP 829 DI 10.2134/agronj2015.0258 PG 8 WC Agronomy SC Agriculture GA DL1XA UT WOS:000375425300034 ER PT J AU Brabec, D Pearson, T Flinn, PW AF Brabec, D. Pearson, T. Flinn, P. W. TI Detection of Lesser Grain Borer Larvae in Internally Infested Kernels of Brown Rice and Wheat Using an Electrically Conductive Roller Mill SO CEREAL FOODS WORLD LA English DT Article ID WEEVIL SITOPHILUS-GRANARIUS; INSECT INFESTATIONS; X-RAY AB Modifications were made to a small laboratory mill to enable the detection of rice kernels internally infested by immature grain insects. The mill, which was originally designed for wheat, monitors electrical conductance through the grain and detects kernels that are infested with live insects based on abrupt changes in electrical conductance as the insects inside the kernels are crushed between the mill rolls. The mill was adapted to detect rice infested by immature lesser grain borers (LGB) by altering the gearing and reducing the gap between the two mill rolls to produce shear between the rolls. Samples of LGB infested long, medium, and short grain (dehulled) brown rice and hard red winter wheat were tested in both the modified and original mills. The detection rates for long grain brown rice kernels infested with large, medium, and small LGB larvae were 97, 83, and 42%, respectively, with the modified mill and 61, 22, and 4%, respectively, with the original mill. Similar detection rates were observed for medium and short grain brown rice with the modified mill. The detection rates for hard red winter wheat kernels infested by large, medium, and small LGB larvae were 98, 94, and 78%, respectively, with the modified mill and 78, 67, and 38%, respectively, with the original mill. More time was required to process a sample through the modified mill than through the original mill. For rice, a 500 g sample could be processed in approximate to 150 sec, making the instrument useful for quality control checks of incoming and outgoing product and for monitoring grain during storage to determine whether fumigation is necessary. However, for drier wheat kernels, the flattened teeth in the modified mill allowed kernel slippage; as a result, the benefit of increased accuracy might not outweigh potential feeding issues. C1 [Brabec, D.; Pearson, T.; Flinn, P. W.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Brabec, D (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. NR 14 TC 0 Z9 0 U1 1 U2 2 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0146-6283 J9 CEREAL FOOD WORLD JI Cereal Foods World PD MAR-APR PY 2016 VL 61 IS 2 BP 65 EP 70 DI 10.1094/CPLEX-2012-0316-01R PG 6 WC Food Science & Technology SC Food Science & Technology GA DK8JN UT WOS:000375173100003 ER PT J AU Jorg, M Simon, T Roland, B Khosro, ST Hassan, VB Sebastian, S Michael, DU Martin, MG AF Joerg, Mueller Simon, Thorn Roland, Baier Khosro, Sagheb-Talebi Hassan, Barimani, V Sebastian, Seibold Michael, Ulyshen D. Martin, Gossner M. TI Protecting the Forests While Allowing Removal of Damaged Trees may Imperil Saproxylic Insect Biodiversity in the Hyrcanian Beech Forests of Iran SO CONSERVATION LETTERS LA English DT Article DE Beech forests; near-to-nature forestry; endemics; Urwald relict species; temperate forests; rapid biodiversity assessment ID FAGUS-ORIENTALIS FORESTS; SPECIES RICHNESS; NORTHERN IRAN; DEAD-WOOD; BEETLES; DIVERSITY; THRESHOLDS; ABUNDANCE; NORWAY; EUROPE AB The 1.8 million ha of forest south of the Caspian Sea represent a remarkably intact ecosystem with numerous old-growth features and unique species assemblages. To protect these forests, Iranian authorities recently passed a law which protects healthy trees but permits the removal of injured, dying and dead trees. To quantify the biodiversity effects of this strategy, we sampled saproxylic beetles and true bugs in 24 plots across the entire altitudinal gradient of Oriental beech. The composition of these communities as well as their overall richness and the richness of endemic and old-growth indicator species were best explained by dead wood volume compared to other environmental variables. Due to the striking evidence that dead wood is the major driver of saproxylic diversity in these forests, we urge Iranian authorities to reconsider their law, redirecting logging toward healthy medium sized trees. Otherwise, a major loss in biodiversity, similar to that experienced in European beech forests, can be expected. C1 [Joerg, Mueller; Simon, Thorn; Sebastian, Seibold] Bavarian Forest Natl Pk, Freyunger Str 2, D-94481 Grafenau, Germany. [Joerg, Mueller; Simon, Thorn; Sebastian, Seibold; Martin, Gossner M.] Tech Univ Munich, Ctr Food & Life Sci Weihenstephan, Terr Ecol Res Grp, Dept Ecol & Ecosyst Management, Hans Carl von Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. [Roland, Baier] Bavarian Off Forest Seeding & Planting ASP, Forstamtspl 1, D-83317 Teisendorf, Germany. [Khosro, Sagheb-Talebi] Res Inst Forests & Rangelands, POB 13185-116, Tehran, Iran. [Hassan, Barimani, V] Agr & Nat Resources Res Ctr Mazandaran, POB 48175-556, Sari, Iran. [Michael, Ulyshen D.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Jorg, M (reprint author), Bavarian Forest Natl Pk, Freyunger Str 2, D-94481 Grafenau, Germany. EM joerg.mueller@npv-bw.bayern.de RI Gossner, Martin M./J-2730-2015 OI Gossner, Martin M./0000-0003-1516-6364 NR 38 TC 1 Z9 1 U1 3 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-263X J9 CONSERV LETT JI Conserv. Lett. PD MAR-APR PY 2016 VL 9 IS 2 BP 106 EP 113 DI 10.1111/conl.12187 PG 8 WC Biodiversity Conservation SC Biodiversity & Conservation GA DK2XV UT WOS:000374778600004 ER PT J AU Adalsteinsson, SA D'Amico, V Shriver, WG Brisson, D Buler, JJ AF Adalsteinsson, Solny A. D'Amico, Vincent Shriver, W. Gregory Brisson, Dustin Buler, Jeffrey J. TI Scale-dependent effects of nonnative plant invasion on host-seeking tick abundance SO ECOSPHERE LA English DT Article DE forest fragmentation; invasive species; Ixodes scapularis; nonnative plant; Rosa multiflora; scale ID IXODES-SCAPULARIS ACARI; AMBLYOMMA-AMERICANUM ACARI; GEOGRAPHIC INFORMATION-SYSTEMS; CENTRAL NEW-YORK; LYME-DISEASE; HABITAT ASSOCIATIONS; REGRESSION TREES; UNITED-STATES; LEAF-LITTER; HUMAN RISK AB Nonnative, invasive shrubs can affect human disease risk through direct and indirect effects on vector populations. Multiflora rose (Rosa multiflora) is a common invader within eastern deciduous forests where tick-borne disease (e.g., Lyme disease) rates are high. We tested whether R.multiflora invasion affects blacklegged tick (Ixodes scapularis) abundance and at what scale. We sampled host-seeking ticks at two spatial scales: fine scale, within R.multiflora-invaded forest fragments; and patch scale, among R.multiflora-invaded and R.multiflora-free forest fragments. At a fine scale, we trapped 2.3 times more ticks under R.multiflora compared with paired traps 25m away from R.multiflora. At the patch scale, we trapped 3.2 times as many ticks in R.multiflora-free forests compared with R.multiflora-invaded forests. Thus, ticks are concentrated beneath R.multiflora within invaded forests, but uninvaded forests support significantly more ticks. Among all covariates tested, leaf litter volume was the best predictor of tick abundance; at the patch scale, R.multiflora-invaded forests had less leaf litter than uninvaded forests. We suggest that leaf litter availability at the patch scale plays a greater role in constraining tick abundance than the fine-scale, positive effect of invasive shrubs. C1 [Adalsteinsson, Solny A.; Shriver, W. Gregory; Buler, Jeffrey J.] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. [D'Amico, Vincent] USDA, No Res Stn, Newark, DE 19716 USA. [Brisson, Dustin] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA. RP Adalsteinsson, SA (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. EM solny@udel.edu OI Buler, Jeffrey/0000-0002-2696-847X FU University of Delaware Graduate Research Fellowship; USDA Forest Service [NRS-08]; USDA McIntire Stennis; University of Delaware; National Institutes of Health [AI076342, AI097137]; National Science Foundation [DEB-1354184]; Burroughs Wellcome Fund [1012376] FX SAA was supported by a University of Delaware Graduate Research Fellowship. Other financial support was provided by the USDA Forest Service NRS-08 (VD), USDA McIntire Stennis (WGS), and University of Delaware (JJB). DB was supported by National Institutes of Health (AI076342 and AI097137), National Science Foundation (DEB-1354184), and the Burroughs Wellcome Fund (1012376). We thank the following individuals who helped collect field data: Z. Ladin, K. Handley, J. Nimmerichter, A. Lutto, K. Serno, J. Bondi, C. Piazza, L. Newton, and J. Curry. We thank R. Falco and B. Kensinger for valuable input on CO2 trap design and acknowledge the cooperation by land managers of sites where we conducted field work: Newark City Parks, New Castle County Parks, University of Delaware, Mt. Cuba Center, and Delaware State Parks. NR 52 TC 0 Z9 0 U1 8 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2016 VL 7 IS 3 AR e01317 DI 10.1002/ecs2.1317 PG 9 WC Ecology SC Environmental Sciences & Ecology GA DK4OB UT WOS:000374896800041 ER PT J AU Duran, J Morse, JL Groffman, PM Campbell, JL Christenson, LM Driscoll, CT Fahey, TJ Fisk, MC Likens, GE Melillo, JM Mitchell, MJ Templer, PH Vadeboncoeur, MA AF Duran, Jorge Morse, Jennifer L. Groffman, Peter M. Campbell, John L. Christenson, Lynn M. Driscoll, Charles T. Fahey, Timothy J. Fisk, Melany C. Likens, Gene E. Melillo, Jerry M. Mitchell, Myron J. Templer, Pamela H. Vadeboncoeur, Matthew A. TI Climate change decreases nitrogen pools and mineralization rates in northern hardwood forests SO ECOSPHERE LA English DT Article DE carbon; global change; microbial activity mineralization; nitrification; nitrogen; soil frost ID CONIFER FOREST; NEW-HAMPSHIRE; DYNAMICS; SOILS; ECOSYSTEMS; CYCLE; TERRESTRIAL; LIMITATION; ROOT; CONSEQUENCES AB Nitrogen (N) supply often limits the productivity of temperate forests and is regulated by a complex mix of biological and climatic drivers. In excess, N is linked to a variety of soil, water, and air pollution issues. Here, we use results from an elevation gradient study and historical data from the long-term Hubbard Brook Ecosystem Study (New Hampshire, USA) to examine relationships between changes in climate, especially during winter, and N supply to northern hardwood forest ecosystems. Low elevation plots with less snow, more soil freezing, and more freeze/thaw cycles supported lower rates of N mineralization than high elevation plots, despite having higher soil temperatures and no consistent differences in soil moisture during the growing season. These results are consistent with historical analyses showing decreases in rates of soil N mineralization and inorganic N concentrations since 1973 that are correlated with long-term increases in mean annual temperature, decreases in annual snow accumulation, and a increases in the number of winter thawing degree days. This evidence suggests that changing climate may be driving decreases in the availability of a key nutrient in northern hardwood forests, which could decrease ecosystem production but have positive effects on environmental consequences of excess N. C1 [Duran, Jorge; Groffman, Peter M.; Likens, Gene E.] Cary Inst Ecosyst Studies, 2801 Sharon Turnpike, Millbrook, NY 12545 USA. [Morse, Jennifer L.] Portland State Univ, Environm Sci & Management, POB 751 ESM, Portland, OR 97207 USA. [Campbell, John L.] US Forest Serv, No Res Stn, USDA, 271 Mast Rd, Durham, NH 03824 USA. [Christenson, Lynn M.] Vassar Coll, Dept Biol, 124 Raymond Ave, Poughkeepsie, NY 12604 USA. [Driscoll, Charles T.] Syracuse Univ, Dept Civil & Environm Engn, 900 S Crouse Ave, Syracuse, NY 13210 USA. [Fahey, Timothy J.] Cornell Univ, Dept Nat Resources, Ithaca, NY 14850 USA. [Fisk, Melany C.] Miami Univ, Dept Zool, 501 E High St, Oxford, OH 45056 USA. [Likens, Gene E.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Melillo, Jerry M.] Ecosyst Ctr, Marine Ecol Lab, Woods Hole, MA 02543 USA. [Mitchell, Myron J.] SUNY Coll Environm Sci & Forestry, Dept Environm & Forest Biol, 1 Forestry Dr, Syracuse, NY 13210 USA. [Templer, Pamela H.] Boston Univ, Dept Biol, 5 Cummington St, Boston, MA 02215 USA. [Vadeboncoeur, Matthew A.] Univ New Hampshire, Earth Syst Res Ctr, Inst Study Earth Oceans & Space, 8 Coll Rd, Durham, NH 03824 USA. [Duran, Jorge] Univ Coimbra, Ctr Funct Ecol, P-3000456 Coimbra, Portugal. [Duran, Jorge] Univ Coimbra, Dept Life Sci, Ctr Funct Ecol, P-3001401 Coimbra, Portugal. RP Duran, J (reprint author), Cary Inst Ecosyst Studies, 2801 Sharon Turnpike, Millbrook, NY 12545 USA.; Duran, J (reprint author), Univ Coimbra, Ctr Funct Ecol, P-3000456 Coimbra, Portugal.; Duran, J (reprint author), Univ Coimbra, Dept Life Sci, Ctr Funct Ecol, P-3001401 Coimbra, Portugal. EM humia20@gmail.com OI Driscoll, Charles/0000-0003-2692-2890; Morse, Jennifer/0000-0001-8872-4940 FU U.S. National Science Foundation; Andrew W. Mellon Foundation; Fulbright fellowship of the Spanish Ministry of Education; FCT Research Fellowship of the Portuguese Ministry of Education and Science [SFRH/BDP/87966/2012] FX This research was supported by grants from the U.S. National Science Foundation programs in Ecosystem Studies, Long-Term Ecological Research and Long-Term Ecological Research in Environmental Biology and from the Andrew W. Mellon Foundation. J.D. was supported by a Fulbright fellowship of the Spanish Ministry of Education and by a FCT Research Fellowship of the Portuguese Ministry of Education and Science (SFRH/BDP/87966/2012). NR 47 TC 3 Z9 3 U1 18 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2016 VL 7 IS 3 AR e01251 DI 10.1002/ecs2.1251 PG 13 WC Ecology SC Environmental Sciences & Ecology GA DK4OB UT WOS:000374896800018 ER PT J AU Pepin, KM Davis, AJ Beasley, J Boughton, R Campbell, T Cooper, SM Gaston, W Hartley, S Kilgo, JC Wisely, SM Wyckoff, C VerCauteren, KC AF Pepin, Kim M. Davis, Amy J. Beasley, James Boughton, Raoul Campbell, Tyler Cooper, Susan M. Gaston, Wes Hartley, Steve Kilgo, John C. Wisely, Samantha M. Wyckoff, Christy VerCauteren, Kurt C. TI Contact heterogeneities in feral swine: implications for disease management and future research SO ECOSPHERE LA English DT Article DE contact; disease transmission; feral swine; GPS; meta-analysis; network; social structure; Sus scrofa ID WILD BOARS; EPIDEMIOLOGY; TRANSMISSION; NETWORKS; PATTERNS; CATTLE; MODELS; VIRUS; TEXAS; PIGS AB Contact rates vary widely among individuals in socially structured wildlife populations. Understanding the interplay of factors responsible for this variation is essential for planning effective disease management. Feral swine (Sus scrofa) are a socially structured species which pose an increasing threat to livestock and human health, and little is known about contact structure. We analyzed 11 GPS data sets from across the United States to understand the interplay of ecological and demographic factors on variation in co-location rates, a proxy for contact rates. Between-sounder contact rates strongly depended on the distance among home ranges (less contact among sounders separated by >2 km; negligible between sounders separated by >6 km), but other factors causing high clustering between groups of sounders also seemed apparent. Our results provide spatial parameters for targeted management actions, identify data gaps that could lead to improved management and provide insight on experimental design for quantitating contact rates and structure. C1 [Pepin, Kim M.; Davis, Amy J.; VerCauteren, Kurt C.] USDA, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80526 USA. [Beasley, James] Univ Georgia, Savannah River Ecol Lab, Warnell Sch Forestry & Nat Resources, PO Drawer E, Aiken, SC 29802 USA. [Boughton, Raoul] Univ Florida, Wildlife Ecol & Conservat, Range Cattle Res & Educ Ctr, 3401 Expt Stn, Ona, FL 33865 USA. [Campbell, Tyler] East Fdn, 200 Concord Plaza Dr,Suite 410, San Antonio, TX 78216 USA. [Cooper, Susan M.] Texas A&M AgriLife Res, 1619 Garner Field Rd, Uvalde, TX 78801 USA. [Gaston, Wes] USDA APHIS Wildlife Serv, Auburn, AL 36849 USA. [Hartley, Steve] US Geol Survey, Natl Wetlands Res Ctr, 700 Cajundome Blvd, Lafayette, LA 70506 USA. [Kilgo, John C.] US Forest Serv, Southern Res Stn, USDA, POB 700, New Ellenton, SC 29809 USA. [Wisely, Samantha M.] Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL 32611 USA. [Wyckoff, Christy] Santa Lucia Conservancy, 26700 Rancho San Carlos Rd, Carmel, CA 93923 USA. [Wyckoff, Christy] Texas A&M Univ Kingsville, Caesar Kleberg Wildlife Res Inst, 955 Univ Blvd, Kingsville, TX 78363 USA. RP Pepin, KM (reprint author), USDA, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80526 USA. EM kim.m.pepin@aphis.usda.gov FU U.S. Department of Agriculture National Wildlife Research Center; U.S. Department of Energy [DE-FC09-07SR22506, DE-AI09-00SR22188]; U.S. Department of Agriculture Forest Service Savannah River; USDA NWRC [1274120896CA] FX We thank two anonymous reviewers for their careful, insightful reviews of an earlier version of this manuscript. Funding was provided by the U.S. Department of Agriculture National Wildlife Research Center and the U.S. Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation and under Interagency Agreement No. DE-AI09-00SR22188 with the U.S. Department of Agriculture Forest Service Savannah River. USDA NWRC provided funding to University of Florida under USDA APHIS Cooperative Agreement 1274120896CA. NR 26 TC 2 Z9 2 U1 8 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2016 VL 7 IS 3 AR e01230 DI 10.1002/ecs2.1230 PG 11 WC Ecology SC Environmental Sciences & Ecology GA DK4OB UT WOS:000374896800009 ER PT J AU Sesnie, SE Mueller, JM Lehnen, SE Rowin, SM Reidy, JL Thompson, FR AF Sesnie, Steven E. Mueller, James M. Lehnen, Sarah E. Rowin, Scott M. Reidy, Jennifer L. Thompson, Frank R., III TI Airborne laser altimetry and multispectral imagery for modeling Golden-cheeked Warbler (Setophaga chrysoparia) density SO ECOSPHERE LA English DT Article DE density models; distance sampling; endangered species; LiDAR; NAIP imagery; point-count surveys; remote sensing ID POINT-COUNT SURVEYS; REPRODUCTIVE SUCCESS; DENDROICA-CHRYSOPARIA; REDUCTION TREATMENTS; RURAL LANDSCAPES; CENTRAL TEXAS; LIDAR DATA; FOREST; OCCUPANCY; HETEROGENEITY AB Robust models of wildlife population size, spatial distribution, and habitat relationships are needed to more effectively monitor endangered species and prioritize habitat conservation efforts. Remotely sensed data such as airborne laser altimetry (LiDAR) and digital color infrared (CIR) aerial photography combined with well-designed field studies can help fill these information voids. We used point count-based distance sampling survey data and LiDAR-fused CIR aerial photography to model density of the Golden-cheeked Warbler (Setophaga chrysoparia), an endangered songbird, on the 10000-ha Balcones Canyonlands National Wildlife Refuge (BCNWR). We developed a novel set of candidate models to explain Golden-cheeked Warbler detection probability and density using habitat covariates characterizing vegetation structure, composition, and complexity as well as habitat fragmentation, topography, and human infrastructure. We had the most model support for covariates calculated using focal means representing a 3.2ha territory size (100 m radius) vs. 1.8 and 7.0 ha territory sizes. Detection probability decreased with canopy cover and increased with topographic roughness. Golden-cheeked Warbler density increased with canopy cover, was highest at a 7:3 ratio of Ashe juniper (Juniperus ashei) to broadleaf tree canopy cover, and decreased with global solar radiation. Predicted warbler densities using 3 min point counts were similar to six estimates from independently collected warbler territory mapping on BCNWR with a mean difference of 6% and a Root Mean Squared Error of 1.88 males/40 ha. The total population size for BCNWR was estimated at 884 Golden-cheeked Warbler males (95% CI 662, 1206) and predicted densities across the refuge ranged from 0.0 to 0.50 male warblers per ha. On the basis of observed habitat relationships, we defined high quality habitat as having at least 60% canopy cover with Ashe juniper comprising 50-90% of the canopy. We estimated 48% of the area at BCNWR managed for Golden-cheeked Warblers was in high quality habitat conditions and identified patches within the lower habitat quality areas (14% of warbler management areas) that had the greatest potential to become high quality habitat with management. Our approach combined robust wildlife surveys with highly scalable remotely sensed data to examine habitat relationships, estimate population size, and identify existing areas of high quality habitat. This method can be applied to other species of conservation interest and can be used with multiple years of remotely sensed data to assess changes in habitat at local to regional scales. C1 [Sesnie, Steven E.; Lehnen, Sarah E.] US Fish & Wildlife Serv Southwest Reg, Div Biol Sci, POB 1306, Albuquerque, NM 87103 USA. [Mueller, James M.; Rowin, Scott M.] US Fish & Wildlife Serv, Balcones Canyonlands Natl Wildlife Refuge, 24518 E FM 1431, Marble Falls, TX 78654 USA. [Reidy, Jennifer L.] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Thompson, Frank R., III] US Forest Serv, USDA, No Res Stn, 202 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. RP Sesnie, SE (reprint author), US Fish & Wildlife Serv Southwest Reg, Div Biol Sci, POB 1306, Albuquerque, NM 87103 USA. EM Steven_Sesnie@fws.gov FU U.S. Fish and Wildlife Service Southwest Region Inventory and Monitoring Initiative FX This study was supported by the U.S. Fish and Wildlife Service Southwest Region Inventory and Monitoring Initiative. We thank Balcones Canyonlands National Wildlife Refuge manager D. Holle and staff for their support with all phases of this project. We thank technicians R. Davis, E. Haeuser, D. Morgan, and S. Shipper for their dedication to supporting field data collection and their attention to detail during point-count surveys. We also thank two anonymous reviewers for providing comments that contributed to developing an improved version of this article. The findings and conclusions in this manuscript are those of the authors and do not necessarily represent the views of the US Fish and Wildlife Service. The use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 69 TC 1 Z9 1 U1 6 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAR PY 2016 VL 7 IS 3 AR e01220 DI 10.1002/ecs2.1220 PG 19 WC Ecology SC Environmental Sciences & Ecology GA DK4OB UT WOS:000374896800004 ER PT J AU Hitaj, C Stocking, A AF Hitaj, Claudia Stocking, Andrew TI Market efficiency and the US market for sulfur dioxide allowances SO ENERGY ECONOMICS LA English DT Article DE Sulfur dioxide market; Efficiency; Volatility; Communication policy ID MONETARY-POLICY; CLIMATE POLICY; INVESTMENT; COMMUNICATION; UNCERTAINTY; VOLATILITY; SO2 AB Focusing on the U.S. sulfur dioxide (SO2) allowance market from its inception in 1994 to 2009, we model allowance prices to determine the influence of market fundamentals on allowance price level and volatility. We find evidence that the SO2 market operates in ways that are not inconsistent with an efficient market - prices that reflect marginal abatement costs - after the first few years of the program but before a court decision that introduced significant uncertainty into the market in mid-2008. Our empirical analysis finds that the SO2 market, similar to other emission markets studied in the literature, can remain relatively inefficient for several years after launch. We also find that market volatility increases in response to all types of communications from the administrator, suggesting that the development of a formal communication strategy, possibly similar to that used by central banks, would reduce price volatility and increase the efficiency of the market. Published by Elsevier B.V. C1 [Hitaj, Claudia] USDA, Econ Res Serv, Washington, DC USA. [Stocking, Andrew] Congress Budget Off, Washington, DC USA. RP Hitaj, C (reprint author), USDA, Econ Res Serv, Washington, DC USA.; Stocking, A (reprint author), Congress Budget Off, Washington, DC USA. EM cmhitaj@ers.usda.gov; astocking@gmail.com NR 52 TC 0 Z9 0 U1 4 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0140-9883 EI 1873-6181 J9 ENERG ECON JI Energy Econ. PD MAR PY 2016 VL 55 BP 135 EP 147 DI 10.1016/j.eneco.2016.01.009 PG 13 WC Economics SC Business & Economics GA DL0YI UT WOS:000375358100013 ER PT J AU Everard, CD Kim, MS Cho, H O'Donnell, CP AF Everard, Colm D. Kim, Moon S. Cho, Hyunjeong O'Donnell, Colm P. TI Hyperspectral fluorescence imaging using violet LEDs as excitation sources for fecal matter contaminate identification on spinach leaves SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE Hyperspectral imaging; Fluorescence; Contaminant detection; Spinach leaves ID ESCHERICHIA-COLI; PLANT STRESS; REFLECTANCE; CLASSIFICATION; QUALITY; O157H7; SAFETY; APPLES; SYSTEM; LEAF AB Food safety in the production of fresh produce for human consumption is a worldwide issue and needs to be addressed to decrease foodborne illnesses and resulting costs. Hyperspectral fluorescence imaging coupled with multivariate image analysis techniques for detection of fecal contaminates on spinach leaves (Spinacia oleracea) was evaluated. Violet fluorescence excitation was provided at 405 nm and light emission was recorded from 464 to 800 nm. Partial least square discriminant analysis and wavelength ratio methods were compared for detection accuracy for fecal contamination. Fluorescence emission profiles of spinach leaves were monitored over a 27 days storage period; peak emission blue-shifts were observed over the storage period accompanying a color change from green to green-yellow-brown hue. The PLSDA model developed correctly detected fecal contamination on 100 % of relatively fresh green spinach leaves used in this investigation, which also had soil contamination. The PLSDA model had 19 % false positives for non-fresh post storage leaves. A wavelength ratio technique using four wavebands (680, 688, 703 and 723 nm) was successful in identifying 100 % of fecal contaminates on both fresh and non-fresh leaves. An on-line fluorescence imaging inspection system for fecal contaminant detection has potential to allow fresh produce producers to reduce foodborne illnesses and prevent against the associated economic losses. C1 [Everard, Colm D.; O'Donnell, Colm P.] Univ Coll Dublin, Sch Biosyst Engn, Dublin 4, Ireland. [Kim, Moon S.] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Cho, Hyunjeong] Natl Agr Prod Qual Management Serv, Expt & Res Inst, 5-3 Gimcheon Innoc, Gimcheon City, Gyeongsangbuk D, South Korea. RP Everard, CD (reprint author), Univ Coll Dublin, Sch Biosyst Engn, Dublin 4, Ireland. EM colm.everard@ucd.ie FU European Union's Seventh Framework Programme (FP7) under the Marie Curie International Outgoing Fellowships for Career Development (FP7-PEOPLE-IOF) FX This publication has emanated from research conducted with the financial support of the European Union's Seventh Framework Programme (FP7) under the Marie Curie International Outgoing Fellowships for Career Development (FP7-PEOPLE-2011-IOF). NR 37 TC 1 Z9 1 U1 6 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD MAR PY 2016 VL 10 IS 1 BP 56 EP 63 DI 10.1007/s11694-015-9276-x PG 8 WC Food Science & Technology SC Food Science & Technology GA DL2GB UT WOS:000375450400007 ER PT J AU Saito, S Margosan, D Michailides, TJ Xiao, CL AF Saito, S. Margosan, D. Michailides, T. J. Xiao, C. L. TI Botrytis californica, a new cryptic species in the B. cinerea species complex causing gray mold in blueberries and table grapes SO MYCOLOGIA LA English DT Article DE cryptic species; gray mold; phylogenetic analysis; plant pathogen; Vaccinum corymbosum; Vitis vinifera ID FRENCH VINEYARDS; PSEUDOCINEREA; POPULATIONS; RESISTANCE; PATHOGEN; CHINA AB The Botrytis cinerea species complex comprises two cryptic species, originally referred to Group I and Group II based on Bc-hch gene RFLP haplotyping. Group I was described as a new cryptic species B. pseudocinerea. During a survey of Botrytis spp. causing gray mold in blueberries and table grapes in the Central Valley of California, six isolates, three from blueberries and three from table grapes, were placed in Group I but had a distinct morphological character with conidiophores significantly longer than those of B. cinerea and B. pseudocinerea. We compared these with B. cinerea and B. pseudocinerea by examining morphological and physiological characters, sensitivity to fenhexamid and phylogenetic analysis inferred from sequences of three nuclear genes. Phylogenetic analysis with the three partial gene sequences encoding glyceraldehyde-3-phosate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60) and DNA-dependent RNA polymerase subunit II (RPB2) supported the proposal of a new Botrytis species, B. californica, which is closely related genetically to B. cinerea, B. pseudocinerea and B. sinoviticola, all known as causal agents of gray mold of grapes. Botrytis californica caused decay on blueberry and table grape fruit inoculated with the fungus. This study suggests that B. califomica is a cryptic species sympatric with B. cinerea on blueberries and table grapes in California. C1 [Saito, S.; Margosan, D.; Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Michailides, T. J.] Univ Calif, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov FU California Table Grape Commission FX We thank S. Pelham for technical support and F. Dugan for pre-submission review. This research was supported in part by the California Table Grape Commission. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 24 TC 4 Z9 5 U1 8 U2 13 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAR-APR PY 2016 VL 108 IS 2 BP 330 EP 343 DI 10.3852/15-165 PG 14 WC Mycology SC Mycology GA DK9GT UT WOS:000375239600007 PM 26740541 ER PT J AU Bergelson, J Buckler, ES Ecker, JR Nordborg, M Weigel, D AF Bergelson, Joy Buckler, Edward S. Ecker, Joseph R. Nordborg, Magnus Weigel, Detlef TI A Proposal Regarding Best Practices for Validating the Identity of Genetic Stocks and the Effects of Genetic Variants SO PLANT CELL LA English DT Letter ID ARABIDOPSIS-THALIANA ACCESSIONS; CELL-LINES; GENOME; MUTATIONS; REVEALS; MUTANT; MAIZE C1 [Bergelson, Joy] Univ Chicago, Chicago, IL 60637 USA. [Buckler, Edward S.] Cornell Univ, USDA ARS, Ithaca, NY 14850 USA. [Ecker, Joseph R.] Salk Inst Biol Studies, HHMI, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA. [Nordborg, Magnus] Gregor Mendel Inst, A-1030 Vienna, Austria. [Weigel, Detlef] Max Planck Inst Dev Biol, D-72076 Tubingen, Germany. RP Weigel, D (reprint author), Max Planck Inst Dev Biol, D-72076 Tubingen, Germany. EM weigel@weigelworld.org RI Weigel, Detlef/C-1418-2008 OI Weigel, Detlef/0000-0002-2114-7963 NR 35 TC 2 Z9 2 U1 3 U2 17 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD MAR PY 2016 VL 28 IS 3 BP 606 EP 609 DI 10.1105/tpc.15.00502 PG 4 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA DL1TK UT WOS:000375415100005 PM 26956491 ER PT J AU Chitwood, DH Rundell, SM Li, DY Woodford, QL Yu, TT Lopez, JR Greenblatt, D Kang, J Londo, JP AF Chitwood, Daniel H. Rundell, Susan M. Li, Darren Y. Woodford, Quaneisha L. Yu, Tommy T. Lopez, Jose R. Greenblatt, Daniel Kang, Julie Londo, Jason P. TI Climate and Developmental Plasticity: Interannual Variability in Grapevine Leaf Morphology SO PLANT PHYSIOLOGY LA English DT Article ID PHENOTYPIC PLASTICITY; ANGIOSPERM LEAVES; NATURAL VARIATION; COMPOUND LEAF; HOMEOBOX GENE; ELEVATED CO2; PHASE-CHANGE; SHAPE; WINE; EXPRESSION AB The shapes of leaves are dynamic, changing over evolutionary time between species, within a single plant producing different shaped leaves at successive nodes, during the development of a single leaf as it allometrically expands, and in response to the environment. Notably, strong correlations between the dissection and size of leaves with temperature and precipitation exist in both the paleorecord and extant populations. Yet, a morphometric model integrating evolutionary, developmental, and environmental effects on leaf shape is lacking. Here, we continue a morphometric analysis of >5,500 leaves representing 270 grapevines of multiple Vitis species between two growing seasons. Leaves are paired one-to-one and vine-to-vine accounting for developmental context, between growing seasons. Linear discriminant analysis reveals shape features that specifically define growing season, regardless of species or developmental context. The shape feature, a more pronounced distal sinus, is associated with the colder, drier growing season, consistent with patterns observed in the paleorecord. We discuss the implications of such plasticity in a long-lived woody perennial, such as grapevine (Vitis spp.), with respect to the evolution and functionality of plant morphology and changes in climate. C1 [Chitwood, Daniel H.; Rundell, Susan M.; Li, Darren Y.; Woodford, Quaneisha L.; Yu, Tommy T.; Greenblatt, Daniel] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Lopez, Jose R.; Kang, Julie] Univ No Iowa, Dept Biol, Cedar Falls, IA 50614 USA. [Londo, Jason P.] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. [Rundell, Susan M.] Yale Univ, New Haven, CT 06520 USA. [Li, Darren Y.; Yu, Tommy T.] Washington Univ, St Louis, MO 63130 USA. [Woodford, Quaneisha L.] Ft Valley State Univ, Ft Valley, GA 31030 USA. RP Chitwood, DH (reprint author), Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. EM dchitwood@danforthcenter.org RI Chitwood, Daniel/M-2841-2013 OI Chitwood, Daniel/0000-0003-4875-1447 FU Donald Danforth Plant Science Center FX This work was generously supported by start-up funds from the Donald Danforth Plant Science Center. NR 81 TC 2 Z9 2 U1 5 U2 15 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD MAR PY 2016 VL 170 IS 3 BP 1480 EP 1491 DI 10.1104/pp.15.01825 PG 12 WC Plant Sciences SC Plant Sciences GA DL1VI UT WOS:000375420300023 PM 26826220 ER PT J AU Nippgen, F McGlynn, BL Emanuel, RE Vose, JM AF Nippgen, Fabian McGlynn, Brian L. Emanuel, Ryan E. Vose, James M. TI Watershed memory at the Coweeta Hydrologic Laboratory: The effect of past precipitation and storage on hydrologic response SO WATER RESOURCES RESEARCH LA English DT Article DE watershed; water balance; storage; watershed memory; Coweeta; precipitation ID EASTERN WHITE-PINE; INTERANNUAL VARIABILITY; EDDY COVARIANCE; CLIMATE-CHANGE; SOIL-MOISTURE; SOUTHERN APPALACHIANS; STREAMFLOW RESPONSE; RUNOFF SIMULATION; SUBSURFACE-FLOW; UNITED-STATES AB The rainfall-runoff response of watersheds is affected by the legacy of past hydroclimatic conditions. We examined how variability in precipitation affected streamflow using 21 years of daily streamflow and precipitation data from five watersheds at the Coweeta Hydrologic Laboratory in southwestern North Carolina, USA. The gauged watersheds contained both coniferous and deciduous vegetation, dominant north and south aspects, and differing precipitation magnitudes. Lag-correlations between precipitation and runoff ratios across a range of temporal resolutions indicated strong influence of past precipitation (i.e., watershed memory). At all time-scales, runoff ratios strongly depended on the precipitation of previous time steps. At monthly time scales, the influence of past precipitation was detectable for up to 7 months. At seasonal time scales, the previous season had a greater effect on a season's runoff ratio than the same season's precipitation. At annual time scales, the previous year was equally important for a year's runoff ratio than the same year's precipitation. Estimated watershed storage through time and specifically the previous year's storage state was strongly correlated with the residuals of a regression between annual precipitation and annual runoff, partially explaining observed variability in annual runoff in watersheds with deep soils. This effect was less pronounced in the steepest watershed that also contained shallow soils. We suggest that the location of a watershed on a nonlinear watershed-scale storage-release curve can explain differences in runoff during growing and dormant season between watersheds with different annual evapotranspiration. C1 [Nippgen, Fabian; McGlynn, Brian L.] Duke Univ, Nicholas Sch Environm, Dept Earth & Ocean Sci, Durham, NC 27708 USA. [Nippgen, Fabian] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Emanuel, Ryan E.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Vose, James M.] N Carolina State Univ, USDA Forest Serv So Res Stn, Ctr Integrated Forest Sci, Raleigh, NC 27695 USA. RP Nippgen, F (reprint author), Duke Univ, Nicholas Sch Environm, Dept Earth & Ocean Sci, Durham, NC 27708 USA.; Nippgen, F (reprint author), Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. EM fabian.nippgen@duke.edu RI McGlynn, Brian/A-2509-2008; Emanuel, Ryan/C-3796-2012 OI McGlynn, Brian/0000-0001-5266-4894; Emanuel, Ryan/0000-0002-2166-1698 FU NSF [EAR-0837937, EAR-0943640, EAR-0838193]; USDA Forest Service Southern Research Station [12-DG-11330155-082]; American Geophysical Union Horton Research Grant FX This study was funded by NSF grants EAR-0837937, EAR-0943640 to McGlynn and EAR-0838193 to Emanuel, USDA Forest Service Southern Research Station grant 12-DG-11330155-082 to McGlynn and Emanuel, and the 2013 American Geophysical Union Horton Research Grant to Nippgen. We thank Chelcy Ford and Stephanie Laseter from the Coweeta Hydrologic Laboratory for providing historical runoff and precipitation data, and Ge Sun from the USDA Forest Service Southern Research Station for providing the Rao et al. [2011] PET estimates. The precipitation and runoff data used in this paper can be requested from the USDA Forest Service at Coweeta, pan evaporation data and LIDAR data can be downloaded at Coweeta.uga.edu and opentopography.org, respectively. NR 75 TC 3 Z9 3 U1 11 U2 22 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD MAR PY 2016 VL 52 IS 3 BP 1673 EP 1695 DI 10.1002/2015WR018196 PG 23 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DK1WR UT WOS:000374706300007 ER PT J AU Clark, MP Schaefli, B Schymanski, SJ Samaniego, L Luce, CH Jackson, BM Freer, JE Arnold, JR Moore, RD Istanbulluoglu, E Ceola, S AF Clark, Martyn P. Schaefli, Bettina Schymanski, Stanislaus J. Samaniego, Luis Luce, Charles H. Jackson, Bethanna M. Freer, Jim E. Arnold, Jeffrey R. Moore, R. Dan Istanbulluoglu, Erkan Ceola, Serena TI Improving the theoretical underpinnings of process-based hydrologic models SO WATER RESOURCES RESEARCH LA English DT Editorial Material DE hydrologic theory; multiple hypotheses; model evaluation; uncertainty ID DYNAMIC TOPMODEL; WATERSHED THERMODYNAMICS; ALTERNATIVE BLUEPRINT; ENVIRONMENTAL-MODELS; UNIFYING FRAMEWORK; FORESTED CATCHMENT; STORAGE DYNAMICS; RUNOFF PROCESSES; MULTIPLE SCALES; STATE VARIABLES AB In this Commentary, we argue that it is possible to improve the physical realism of hydrologic models by making better use of existing hydrologic theory. We address the following questions: (1) what are some key elements of current hydrologic theory; (2) how can those elements best be incorporated where they may be missing in current models; and (3) how can we evaluate competing hydrologic theories across scales and locations? We propose that hydrologic science would benefit from a model-based community synthesis effort to reframe, integrate, and evaluate different explanations of hydrologic behavior, and provide a controlled avenue to find where understanding falls short. C1 [Clark, Martyn P.] Natl Ctr Atmospher Res, Res Applicat Lab, POB 3000, Boulder, CO 80307 USA. [Schaefli, Bettina] Ecole Polytech Fed Lausanne, Sch Architecture Civil & Environm Engn, Lausanne, Switzerland. [Schaefli, Bettina] Univ Lausanne, Fac Geosci & Environm, CH-1015 Lausanne, Switzerland. [Schymanski, Stanislaus J.] Swiss Fed Inst Technol, Dept Environm Syst Sci, Zurich, Switzerland. [Samaniego, Luis] UFZ Helmholtz Ctr Environm Res, CHS, Leipzig, Germany. [Luce, Charles H.] USDA, Forest Serv, Res & Dev, Boise, ID USA. [Jackson, Bethanna M.] Victoria Univ Wellington, Sch Geog Environm & Earth Sci, Wellington, New Zealand. [Freer, Jim E.] Univ Bristol, Sch Geog Sci, Bristol, Avon, England. [Arnold, Jeffrey R.] US Army Corps Engineers, IWR JISAO, Seattle, WA USA. [Moore, R. Dan] Univ British Columbia, Dept Geog, Vancouver, BC, Canada. [Istanbulluoglu, Erkan] Univ Washington, Coll Engn, Dept Civil & Environm Engn, Seattle, WA 98195 USA. [Ceola, Serena] Univ Bologna, DICAM, Bologna, Italy. RP Clark, MP (reprint author), Natl Ctr Atmospher Res, Res Applicat Lab, POB 3000, Boulder, CO 80307 USA. EM mclark@ucar.edu RI Freer, Jim/C-7335-2009; Samaniego, Luis/G-8651-2011; Schymanski, Stanislaus/A-5303-2012; Schaefli, Bettina/B-2069-2010; Luce, Charles/A-9267-2008 OI Samaniego, Luis/0000-0002-8449-4428; Schymanski, Stanislaus/0000-0002-0950-2942; Schaefli, Bettina/0000-0003-1140-6244; Luce, Charles/0000-0002-6938-9662 NR 144 TC 4 Z9 4 U1 6 U2 9 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD MAR PY 2016 VL 52 IS 3 BP 2350 EP 2365 DI 10.1002/2015WR017910 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DK1WR UT WOS:000374706300043 ER PT J AU Torrez, MA Arendt, WJ Diaz, L AF Torrez, Marvin A. Arendt, Wayne J. Diaz, Luis TI Consumption of Mullerian Bodies by Golden-olive Woodpecker (Colaptes rubiginosus) in Nicaragua's Highlands SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article ID EATING BIRDS; CECROPIA; AZTECA; FOREST; PLANT; TIME; TREE AB The Golden-olive Woodpecker is a generalist species found in a wide range of habitats, being particularly common in coffee plantations within Nicaraguan cloud forests. Observations of an individual feeding at the base of Cecropia leaves revealed it was consuming Mullerian bodies that the Cecropia produces to feed Azteca ants as part of a host-inhabitant mutualistic symbiosis. This record further documents the plasticity of some species as they search for alternative sources of energy. C1 [Torrez, Marvin A.] Univ Ctr Amer, Estn Biol Juan Roberto Zarruk, Rotonda Ruben Dario 150 M Al Oeste,Apdo 69, Managua, Nicaragua. [Arendt, Wayne J.] USDA Forest Serv, Int Inst Trop Forestry, Sabana Field Res Stn, HC 2 Box 6205, Luquillo, PR 00773 USA. [Diaz, Luis] Paso Pacffico Carretera Masaya Km 12-4 Residencia, Managua, Nicaragua. RP Torrez, MA (reprint author), Univ Ctr Amer, Estn Biol Juan Roberto Zarruk, Rotonda Ruben Dario 150 M Al Oeste,Apdo 69, Managua, Nicaragua. EM mtorrez@ns.uca.edu.ni FU USDA-FS/IITF International Cooperation's Program FX We thank the USDA Forest Service, International Institute of Tropical Forestry, Paso Pacifico, and Central American University-Managua for monetary and logistical support during this ongoing project. We also acknowledge and are grateful for the support provided by Mr. Jerry Bauer, USDA-FS/IITF International Cooperation's Program Director, for all his efforts and financial support to ensure the continuation of our long-term biodiversity monitoring. We also thank our driver, Marcos Mercado, for his assistance in the field and his dedication and passion while searching for birds. Part of this research was conducted in cooperation with the University of Puerto Rico. NR 26 TC 0 Z9 0 U1 1 U2 1 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 EI 1938-5447 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD MAR PY 2016 VL 128 IS 1 BP 190 EP 193 PG 4 WC Ornithology SC Zoology GA DK9HR UT WOS:000375242000021 ER PT J AU Gill, RA Cox, WA Thompson, FR AF Gill, Ryan A. Cox, W. Andrew Thompson, Frank R., III TI Timing of Songbird Nest Predation as Revealed by Video Surveillance SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article DE nest predation; nest predator ecology; nest visitation; songbird ecology; video surveillance ID PLASTICITY; RATES; BIRDS; RISK AB The use of video to monitor nests has increased in frequency over the past 25 years and new research using this technology has shed light on many aspects of the interactions between predators and nesting birds. We used video cameras to describe the timing of nest predation events for Acadian Flycatchers (Empidonax virescens), Indigo Buntings (Passerina cyanea), and other forest-dwelling songbird species. Seventy-four percent (111 of 151) of nest predation events occurred during diurnal hours for both focal species. Although some of our observations were unexpected (e.g., Barred Owls [Strix varia] were primarily diurnal nest predators), many of the predator-specific temporal patterns we observed were consistent with prior knowledge. Understanding diel patterns of nest predation in conjunction with identification of the suite of predators that contribute to overall predation rates will improve our understanding of how birds recognize and respond to the risk of nest predation across ecological and evolutionary time scales. C1 [Gill, Ryan A.] Univ Nebraska, Dept Biol, 6001 Dodge St, Omaha, NE 68182 USA. [Cox, W. Andrew] Univ Missouri, Div Biol Sci, 105 Tucker Hall, Columbia, MO 65211 USA. [Thompson, Frank R., III] Univ Missouri, USDA, Forest Serv, No Res Stn, 202 ABNR, Columbia, MO 65211 USA. [Cox, W. Andrew] Florida Fish & Wildlife Conservat Commiss, Fish & Wildlife Res Inst, 1105 SW Williston Rd, Gainesville, FL 32608 USA. RP Cox, WA (reprint author), Univ Missouri, Div Biol Sci, 105 Tucker Hall, Columbia, MO 65211 USA.; Cox, WA (reprint author), Florida Fish & Wildlife Conservat Commiss, Fish & Wildlife Res Inst, 1105 SW Williston Rd, Gainesville, FL 32608 USA. EM andrew.cox@myfwc.com FU U.S.D.A. Forest Service Northern Research Station; Audubon Society of Missouri; Trans-World Airlines Scholarship program; National Science Foundation Graduate Research Fellowship; University of Missouri Life Sciences Fellowship FX Funding for this research was provided by the U.S.D.A. Forest Service Northern Research Station, the Audubon Society of Missouri, and the Trans-World Airlines Scholarship program. W. A. Cox was supported by a National Science Foundation Graduate Research Fellowship and a University of Missouri Life Sciences Fellowship. We thank many field assistants and the Missouri Department of Conservation for providing housing and access to field sites. We thank the Mark Twain National Forest, the Illinois Department of Natural Resources, and the private landowners near Thompsonville, IL for access to their land. W. A. Cox thanks the Fish and Wildlife Research Institute for allowing this manuscript to be completed at the Institute. NR 19 TC 0 Z9 0 U1 5 U2 8 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 EI 1938-5447 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD MAR PY 2016 VL 128 IS 1 BP 200 EP 203 PG 4 WC Ornithology SC Zoology GA DK9HR UT WOS:000375242000024 ER PT J AU Brundage, AL Crippen, TL Tomberlin, JK AF Brundage, Adrienne L. Crippen, Tawni L. Tomberlin, Jeffery K. TI Methods for external disinfection of blow fly (Diptera: Calliphoridae) eggs prior to use in wound debridement therapy SO WOUND REPAIR AND REGENERATION LA English DT Article ID PSEUDOMONAS-AERUGINOSA BIOFILMS; TRANSCUTANEOUS OXYGEN-TENSION; TRANSCRIPTOME ANALYSIS; INFLAMMATORY RESPONSE; MICROARRAY ANALYSIS; STRESS-RESPONSE; GROWTH; MODEL; GENES; IDENTIFICATION AB Maggot debridement therapy (MDT) is the use of the larval stage of flies (i.e., Calliphoridae) to remove necrotic tissue and disinfect wounds. Effective MDT requires aseptic technique to prevent the unintentional introduction of pathogenic bacteria into a wound to be debrided; yet the external surface of Calliphoridae eggs is often heavily contaminated with bacteria. Studies of external disinfection of dipteran eggs have been reported, but neither their efficacy nor effect on egg viability has been adequately assessed. The present study evaluated the efficacy of ten disinfection techniques involving immersion, rinse, or a combination of both in formalin, Lysol, formaldehyde, bleach, ethanol, Sporgon, or benzalkonium chloride. All techniques resulted in significant decreases in culturable, aerobic bacterial load on Lucilia cuprina eggs. For L. cuprina, a 10 minute 3% Lysol immersion was the most efficacious, disinfecting 96.67% of egg samples, while resulting in 31.84% egg mortality. The 5% formalin immersion was least efficacious, disinfecting only 3.33% of L. cuprina egg samples, while resulting in 33.51% egg mortality. A formaldehyde immersion, one of the most commonly used disinfection techniques, was moderately effective, disinfecting 66.7% of egg samples, while resulting in 40.16% egg mortality. For Chrysomya rufifacies and Cochliomyia macellaria egg samples, the 10 minute 3% Lysol immersion disinfected 100% of the samples, and for Lucilia sericata, 80% of egg samples, while resulting in 33.97%, 7.34%, and 36.96% egg mortality, respectively. H2CO disinfected 16.67% of Ch. rufifacies, 26.67% of C. macellaria, and 56.67% of L. sericata egg samples, while resulting in 21.98%, 10.18%, and 32.19% egg mortality, respectively. Due to its high disinfection efficacy and relatively low egg mortality, a 10 minutes 3% Lysol immersion is recommended for sterilizing Calliphoridae eggs prior to rearing of larvae for use in MDT. C1 [Brundage, Adrienne L.; Tomberlin, Jeffery K.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Crippen, Tawni L.] USDA, Southern Plains Agr Res Ctr, Agr Res Serv, College Stn, TX USA. RP Brundage, AL (reprint author), Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. EM adrienne.brundage@email.tamu.edu FU National Institute of Justice, Office of Justice Programs, U.S. Department of Justice [2010-DN-BX-K243] FX Additional funds for TLC and JKT were provided by the National Institute of Justice, Office of Justice Programs, U.S. Department of Justice through Grant 2010-DN-BX-K243. NR 39 TC 1 Z9 1 U1 12 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1067-1927 EI 1524-475X J9 WOUND REPAIR REGEN JI Wound Repair Regen. PD MAR-APR PY 2016 VL 24 IS 2 BP 384 EP 393 DI 10.1111/wrr.12408 PG 10 WC Cell Biology; Dermatology; Medicine, Research & Experimental; Surgery SC Cell Biology; Dermatology; Research & Experimental Medicine; Surgery GA DK8DP UT WOS:000375157700148 PM 27041484 ER PT J AU Edwards, J Prevost, N Yager, D AF Edwards, J. Prevost, N. Yager, D. TI HYDROGEN PEROXIDE PRODUCTION FROM FIBROUS PECTIC CELLULOSE ANALOGS AND EFFECT ON DERMAL FIBROBLASTS SO WOUND REPAIR AND REGENERATION LA English DT Meeting Abstract C1 [Edwards, J.; Prevost, N.] USDA, So Reg Res Ctr, New Orleans, LA 70179 USA. [Yager, D.] Virginia Commonwealth Univ, Richmond, VA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1067-1927 EI 1524-475X J9 WOUND REPAIR REGEN JI Wound Repair Regen. PD MAR-APR PY 2016 VL 24 IS 2 BP A7 EP A7 PG 1 WC Cell Biology; Dermatology; Medicine, Research & Experimental; Surgery SC Cell Biology; Dermatology; Research & Experimental Medicine; Surgery GA DK8DP UT WOS:000375157700031 ER PT J AU Fontenot, KR Edwards, JV Pircher, N Liebner, F AF Fontenot, K. R. Edwards, J. V. Pircher, N. Liebner, F. TI DESIGN AND ACTIVITY OF POINT OF CARE PROTEASE DETECTION WITH AEROGEL SENSORS DERIVED FROM COTTON SO WOUND REPAIR AND REGENERATION LA English DT Meeting Abstract C1 [Fontenot, K. R.; Edwards, J. V.] USDA, SRRC, Cotton Chem & Utilizat, New Orleans, LA USA. [Pircher, N.; Liebner, F.] Univ Nat Resources & Life Sci, Dept Chem, Vienna, Austria. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1067-1927 EI 1524-475X J9 WOUND REPAIR REGEN JI Wound Repair Regen. PD MAR-APR PY 2016 VL 24 IS 2 BP A8 EP A8 PG 1 WC Cell Biology; Dermatology; Medicine, Research & Experimental; Surgery SC Cell Biology; Dermatology; Research & Experimental Medicine; Surgery GA DK8DP UT WOS:000375157700034 ER PT J AU Padgett-Stewart, TM Wilcox, TM Carim, KJ McKelvey, KS Young, MK Schwartz, MK AF Padgett-Stewart, Ticha M. Wilcox, Taylor M. Carim, Kellie J. McKelvey, Kevin S. Young, Michael K. Schwartz, Michael K. TI An eDNA assay for river otter detection: a tool for surveying a semi-aquatic mammal SO CONSERVATION GENETICS RESOURCES LA English DT Article DE Environmental DNA; River otters; Semi-aquatic mammals; Survey ID ENVIRONMENTAL DNA AB Environmental DNA (eDNA) is an effective tool for the detection of elusive or low-density aquatic organisms. However, it has infrequently been applied to mammalian species. North American river otters (Lontra canadensis) are both broad ranging and semi-aquatic, making them an ideal candidate for examining the uses of eDNA for detection of mammals. We developed a species-specific assay for detection of North American river otters using eDNA. The assay was tested for specificity against closely-related mustelids native to western North America, and was validated through testing environmental samples. C1 [Padgett-Stewart, Ticha M.; Wilcox, Taylor M.; Carim, Kellie J.; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K.] USDA, Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, Missoula, MT USA. [Padgett-Stewart, Ticha M.] Hellgate High Sch, Missoula, MT USA. [Wilcox, Taylor M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Padgett-Stewart, TM (reprint author), USDA, Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, Missoula, MT USA.; Padgett-Stewart, TM (reprint author), Hellgate High Sch, Missoula, MT USA. EM tichaps13@gmail.com OI Padgett-Stewart, Ticha/0000-0002-8367-8989 FU NSF Graduate Research Fellowship [DGE-1313190]; Advanced Problems in Science, Hellgate high school, Missoula, MT, USA FX We thank ZooMontana and Mackenzie Mentel for exhibit samples. We thank Justin Gude, Neil Anderson, Jeff Copeland, Wayne Melquist, Jody Tucker, Kristy Pilgrim, and Joel Sauder for reference samples. We also thank Rob Jensen and Advanced Problems in Science, Hellgate high school, Missoula, MT, USA, for their support. TMW is supported by a NSF Graduate Research Fellowship ( Grant No. DGE-1313190). NR 16 TC 0 Z9 0 U1 14 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1877-7252 EI 1877-7260 J9 CONSERV GENET RESOUR JI Conserv. Genet. Resour. PD MAR PY 2016 VL 8 IS 1 BP 5 EP 7 DI 10.1007/s12686-015-0511-x PG 3 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA DJ6IR UT WOS:000374315600002 ER PT J AU Al-Qudah, OM Liu, FJ Lerch, RN Kitchen, N Yang, J AF Al-Qudah, Omar M. Liu, Fengjing Lerch, Robert N. Kitchen, Newell Yang, John TI Controls on nitrate-N concentrations in groundwater in a Missourian claypan watershed SO EARTH AND SPACE SCIENCE LA English DT Article DE Goodwater Creek Experimental Watershed; nitrate-N; claypan soil; groundwater; spatial variation; temporal variation ID NORTH-CENTRAL MISSOURI; PREFERENTIAL FLOW; DRINKING-WATER; UNITED-STATES; SOIL; RUNOFF; VULNERABILITY; VARIABILITY; PESTICIDES; TRANSPORT AB Nitrogen (N) fertilizer applications have resulted in widespread groundwater nitrate-N (NO3-N) contamination in the U.S. Corn Belt. Goodwater Creek Experimental Watershed (GCEW) is an agricultural watershed in the claypan soil region of northeastern Missouri with a network of 96 wells at depths of 2.7-15.7m. The objectives of this study were to (1) inspect the spatial and temporal variations of NO3-N concentrations in GCEW's groundwater, particularly with well depth at scales ranging from individual well, well nest, and field to the entire watershed during the period 1991 to 2004; (2) understand the processes controlling the variability of NO3-N concentrations in groundwater at various scales within GCEW; and (3) compare groundwater NO3-N concentrations in GCEW to other agricultural watersheds in the U.S. Nitrate-N concentrations were determined in more than 2000 samples collected from 1991 to 2004. Despite the low hydraulic conductivity of the claypan soils, considerable NO3-N contamination of the glacial till aquifer occurred, with 38% of the wells exceeding 10mgL(-1). Groundwater recharge by preferential pathways through the claypan appeared to be the primary mechanism for NO3-N movement to the aquifer. Changes in concentration with depth steadily increased to 8.5-10m and then decreased with further depth. This pattern was consistent with decreased hydraulic conductivity in the Paleosol layer at 8.5-10m, denitrification below this layer, and mixing of recent contaminated water with older uncontaminated water in the lowest strata. Only 19-23% of sampled wells exceeded 10mgL(-1) in nonclaypan agricultural watersheds over the continental U.S., suggesting that groundwater in GCEW was more susceptible to NO3-N contamination than nonclaypan watersheds. These results demonstrated that preferential flow through the soil and hydraulic conductivity of the subsurface strata controlled NO3-N transport in this claypan watershed. Key Points C1 [Al-Qudah, Omar M.; Liu, Fengjing; Yang, John] Lincoln Univ, Dept Agr & Environm Sci, Jefferson City, MO USA. [Al-Qudah, Omar M.; Liu, Fengjing; Yang, John] Lincoln Univ, Cooperat Res Program, Jefferson City, MO USA. [Lerch, Robert N.; Kitchen, Newell] USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. RP Al-Qudah, OM (reprint author), Lincoln Univ, Dept Agr & Environm Sci, Jefferson City, MO USA. EM al-qudaho@lincolnu.edu NR 57 TC 0 Z9 0 U1 4 U2 4 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2333-5084 J9 Earth Space Sci JI Earth Space Sci. PD MAR PY 2016 VL 3 IS 3 BP 90 EP 105 DI 10.1002/2015EA000117 PG 16 WC Geosciences, Multidisciplinary SC Geology GA DJ6TG UT WOS:000374345300001 ER PT J AU Talbot, NC Wang, L Garrett, WM Caperna, TJ Tang, Y AF Talbot, Neil C. Wang, Ling Garrett, Wesley M. Caperna, Thomas J. Tang, Young TI Establishment and characterization of feeder cell-dependent bovine fetal liver cell lines SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Article DE Bovine; Cell line; Feeder cells; Hepatocyte; Liver; STO ID GROWTH-FACTOR-BETA; HEPATOCYTES IN-VITRO; EMBRYONIC STEM-CELLS; PRIMARY CULTURES; PORCINE HEPATOCYTES; EPITHELIAL-CELLS; RAT HEPATOCYTES; TGF-BETA; TRIGLYCERIDE ACCUMULATION; DNA-SYNTHESIS AB The establishment and initial characterization of bovine fetal liver cell lines are described. Bovine fetal hepatocytes were cultured from the liver of a 34-d bovine fetus by physical disruption of the liver tissue. Released liver cells and clumps of cells were plated on STO (SIMS mouse strain, thioguanine-and ouabain-resistant) feeder layers and were cultured in a medium supplemented with 10% fetal bovine serum. After 2-3 wk, primary colonies of hepatocytes were observed by phase-contrast microscopic observation. Individual hepatocyte colonies were colony-cloned into independent bovine fetal liver (BFL) cell lines. Two cell lines, BFL-6 and BFL-9, grew the best of several isolates, and they were further characterized for growth potential and for hepatocyte morphology and function. The two cell lines were found to grow markedly better in the presence of the transforming growth factor (TGF)-beta inhibitor, SB431542 (1 mu M). Their continuous culture also depended on a particular medium height-for T12.5 flasks, 3 ml total medium produced optimum growth. Higher or lower amounts of medium caused less cell growth or cessation of growth. The cell lines were propagated for over a year at split ratios of 1: 2 or 1: 3 at each passage until reaching senescence at approximately 30 passages. The cells were laterally polarized with well-developed canalicular spaces occurring between adjacent BFL cells. Treatment of the cultures with cyclic adenosine monophosphate (cAMP)-stimulating chemicals or peptides (e.g., forskolin or glucagon) caused physical expansion of the canaliculi between the cells within 15 min. The cells secreted a spectrum of serum proteins, were positive for the expression of several hepatocyte-specific genes, and converted ammonia to urea, although at a relatively low rate. The culture system provides an in vitro model of fetal bovine hepatocytes and is the first demonstration of the continuous culture of normal bovine hepatocytes as cell lines. C1 [Talbot, Neil C.; Garrett, Wesley M.; Caperna, Thomas J.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, NEA,USDA,BARC East, Bldg 200,Rm 13, Beltsville, MD 20705 USA. [Wang, Ling; Tang, Young] Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA. RP Talbot, NC (reprint author), ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, NEA,USDA,BARC East, Bldg 200,Rm 13, Beltsville, MD 20705 USA. EM neil.talbot@ars.usda.gov NR 70 TC 0 Z9 0 U1 2 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAR PY 2016 VL 52 IS 3 BP 314 EP 326 DI 10.1007/s11626-015-9982-z PG 13 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DJ6VG UT WOS:000374350700007 PM 26659396 ER PT J AU Chamberlain, SD Gomez-Casanovas, N Walter, MT Boughton, EH Bernacchi, CJ DeLucia, EH Groffman, PM Keel, EW Sparks, JP AF Chamberlain, Samuel D. Gomez-Casanovas, Nuria Walter, M. Todd Boughton, Elizabeth H. Bernacchi, Carl J. DeLucia, Evan H. Groffman, Peter M. Keel, Earl W. Sparks, Jed P. TI Influence of transient flooding on methane fluxes from subtropical pastures SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE methane; eddy covariance; mesocosm; subtropical; pastureland; precipitation ID TROPICAL FOREST SOILS; LANDFILL COVER SOILS; NUTRIENT ENRICHMENT; FLORIDA EVERGLADES; PERMAFROST THAW; EMISSIONS; OXIDATION; BIOGEOCHEMISTRY; CONSUMPTION; FLUCTUATIONS AB Seasonally flooded subtropical pastures are major methane (CH4) sources, where transient flooding drives episodic and high-magnitude emissions from the underlying landscape. Understanding the mechanisms that drive these patterns is needed to better understand pasture CH4 emissions and their response to global change. We investigated belowground CH4 dynamics in relation to surface fluxes using laboratory water table manipulations and compared these results to field-based eddy covariance measurements to link within-soil CH4 dynamics to ecosystem fluxes. Ecosystem CH4 fluxes lag flooding events, and this dynamic was replicated in laboratory experiments. In both cases, peak emissions were observed during water table recession. Flooding of surface organic soils and precipitation driven oxygen pulses best explained the observed time lags. Precipitation oxygen pulses likely delay CH4 emissions until groundwater dissolved oxygen is consumed, and emissions were temporally linked to CH4 production in surface soil horizons. Methane accumulating in deep soils did not contribute to surface fluxes and is likely oxidized within the soil profile. Methane production rates in surface organic soils were also orders of magnitude higher than in deep mineral soils, suggesting that over longer flooding regimes CH4 produced in deep horizons is not a significant component of surface emissions. Our results demonstrate that distinct CH4 dynamics may be stratified by depth and flooding of surface organic soils drives CH4 fluxes from subtropical pastures. These results suggest that small changes in pasture water table dynamics can drive large changes in CH4 emissions if surface soils remain saturated over longer time scales. C1 [Chamberlain, Samuel D.; Sparks, Jed P.] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY USA. [Gomez-Casanovas, Nuria; DeLucia, Evan H.] Univ Illinois, Dept Plant Biol, Urbana, IL USA. [Gomez-Casanovas, Nuria; DeLucia, Evan H.] Univ Illinois, Energy Biosci Inst, Urbana, IL USA. [Gomez-Casanovas, Nuria; DeLucia, Evan H.] Univ Illinois, Inst Genom Biol, Urbana, IL USA. [Walter, M. Todd] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY USA. [Boughton, Elizabeth H.; Keel, Earl W.] MacArthur Agroecol Res Ctr, Lake Placid, FL USA. [Bernacchi, Carl J.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL USA. [Groffman, Peter M.] CUNY, Adv Sci Res Ctr, New York, NY 10021 USA. RP Chamberlain, SD (reprint author), Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY USA. EM sdc84@cornell.edu FU Cornell University's Cross-scale Biogeochemistry and Climate Program; Atkinson Center for a Sustainable Future; Department of Ecology and Evolutionary Biology; Andrew W. Mellon Foundation; Cornell Sigma Xi; University of Illinois USDA ARS FX We thank Hilary Swain, Julia Maki, and the rest of the staff at the MacArthur Agro-ecology Research Center for site access, lodging, transportation, and continued support in the field and thank Suzanne Pierre for help excavating and transporting soil columns. We also thank Mark Conrad for input to analysis and experimental design. This research was supported by Cornell University's Cross-scale Biogeochemistry and Climate Program, Atkinson Center for a Sustainable Future, Department of Ecology and Evolutionary Biology, Andrew W. Mellon Foundation, Cornell Sigma Xi, and University of Illinois USDA ARS. Included in the supporting information are all CH4 eddy covariance and hydrologic (Table S1), mesocosm CH4 (Table S2), mesocosm oxygen (Table S3), and incubation (Table S4) data. NR 50 TC 1 Z9 1 U1 8 U2 16 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD MAR PY 2016 VL 121 IS 3 BP 965 EP 977 DI 10.1002/2015JG003283 PG 13 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DJ6TD UT WOS:000374345000024 ER PT J AU Coop, JD Parks, SA McClernan, SR Holsinger, LM AF Coop, Jonathan D. Parks, Sean A. McClernan, Sarah R. Holsinger, Lisa M. TI Influences of prior wildfires on vegetation response to subsequent fire in a reburned Southwestern landscape SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bandelier National Monument; New Mexico; USA; fire severity; general resilience; Jemez Mountains; New Mexico; USA; landscape memory; ponderosa pine (Pinus ponderosa); prescribed fire; relativized burn ratio ID PONDEROSA PINE FORESTS; QUANTIFYING BURN SEVERITY; ECOLOGICAL RESTORATION; NEW-MEXICO; RESILIENCE; ARIZONA; USA; REGENERATION; COMMUNITIES; MOUNTAINS AB Large and severe wildfires have raised concerns about the future of forested landscapes in the southwestern United States, especially under repeated burning. In 2011, under extreme weather and drought conditions, the Las Conchas fire burned over several previous burns as well as forests not recently exposed to fire. Our purpose was to examine the influences of prior wildfires on plant community composition and structure, subsequent burn severity, and vegetation response. To assess these relationships, we used satellite-derived measures of burn severity and a nonmetric multidimensional scaling of pre- and post-Las Conchas field samples. Earlier burns were associated with shifts from forested sites to open savannas and meadows, oak scrub, and ruderal communities. These non-forested vegetation types exhibited both resistance to subsequent fire, measured by reduced burn severity, and resilience to reburning, measured by vegetation recovery relative to forests not exposed to recent prior fire. Previous shifts toward non-forested states were strongly reinforced by reburning. Ongoing losses of forests and their ecological values confirm the need for restoration interventions. However, given future wildfire and climate projections, there may also be opportunities presented by transformations toward fire-resistant and resilient vegetation types within portions of the landscape. C1 [Coop, Jonathan D.; McClernan, Sarah R.] Western State Colorado Univ, Gunnison, CO 81231 USA. [Parks, Sean A.; Holsinger, Lisa M.] USDA, US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Coop, JD (reprint author), Western State Colorado Univ, Gunnison, CO 81231 USA. EM jcoop@western.edu FU Joint Fire Science Program (JFSP) [12-1-03-19]; Thornton Undergraduate Research Fund at WSCU FX We thank the staff of Bandelier National Monument and the Santa Fe National Forest for access to field study sites. Kay Beeley facilitated field sampling and provided data and reports. Esteban Muldavin and the New Mexico Natural Heritage Program offered invaluable access to pre-Las Conchas data and photos. Ashley Woolman contributed to field sampling in 2013. Dave Roberts shared R code. This project benefited immensely from early help and insight from Sandra Haire and Carol Miller, and we thank Susana Bautista and an anonymous reviewer for detailed and helpful reviews of an earlier version of this manuscript. Funding for J. Coop and S. McClernan was provided by the Thornton Undergraduate Research Fund at WSCU. S. Parks and L. Holsinger acknowledge funding from the Joint Fire Science Program (JFSP 12-1-03-19). NR 36 TC 4 Z9 3 U1 8 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD MAR PY 2016 VL 26 IS 2 BP 346 EP 354 DI 10.1890/15-0775.1 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DJ3PU UT WOS:000374118700002 PM 27209778 ER PT J AU Carver, S Bevins, SN Lappin, MR Boydston, EE Lyren, LM Alldredge, M Logan, KA Sweanor, LL Riley, SPD Serieys, LEK Fisher, RN Vickers, TW Boyce, W McBride, R Cunningham, MC Jennings, M Lewis, J Lunn, T Crooks, KR VandeWoude, S AF Carver, Scott Bevins, Sarah N. Lappin, Michael R. Boydston, Erin E. Lyren, Lisa M. Alldredge, Mathew Logan, Kenneth A. Sweanor, Linda L. Riley, Seth P. D. Serieys, Laurel E. K. Fisher, Robert N. Vickers, T. Winston Boyce, Walter McBride, Roy Cunningham, Mark C. Jennings, Megan Lewis, Jesse Lunn, Tamika Crooks, Kevin R. VandeWoude, Sue TI Pathogen exposure varies widely among sympatric populations of wild and domestic felids across the United States SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bartonella sp.; bobcat (Lynx rufus); cross-species transmission; disease exposure; domestic cat (Felis catus); feline calicivirus; feline herpesvirus-1; feline immunodeficiency virus; feline panleukopenea virus; puma (Puma concolor); Toxoplasma gondii; urbanization ID BOBCATS LYNX-RUFUS; PARASITE SPECIES RICHNESS; LIONS PUMA-CONCOLOR; TOXOPLASMA-GONDII; IMMUNODEFICIENCY VIRUS; LEUKEMIA-VIRUS; LENTIVIRAL INFECTION; DISEASE PREVALENCE; HOST-SPECIFICITY; NATIONAL-PARK AB Understanding how landscape, host, and pathogen traits contribute to disease exposure requires systematic evaluations of pathogens within and among host species and geographic regions. The relative importance of these attributes is critical for management of wildlife and mitigating domestic animal and human disease, particularly given rapid ecological changes, such as urbanization. We screened >1000 samples from sympatric populations of puma (Puma concolor), bobcat (Lynx rufus), and domestic cat (Felis catus) across urban gradients in six sites, representing three regions, in North America for exposure to a representative suite of bacterial, protozoal, and viral pathogens (Bartonella sp., Toxoplasma gondii, feline herpesvirus-1, feline panleukopenea virus, feline calicivirus, and feline immunodeficiency virus). We evaluated prevalence within each species, and examined host trait and land cover determinants of exposure; providing an unprecedented analysis of factors relating to potential for infections in domesticated and wild felids. Prevalence differed among host species (highest for puma and lowest for domestic cat) and was greater for indirectly transmitted pathogens. Sex was inconsistently predictive of exposure to directly transmitted pathogens only, and age infrequently predictive of both direct and indirectly transmitted pathogens. Determinants of pathogen exposure were widely divergent between the wild felid species. For puma, suburban land use predicted increased exposure to Bartonella sp. in southern California, and FHV-1 exposure increased near urban edges in Florida. This may suggest interspecific transmission with domestic cats via flea vectors (California) and direct contact (Florida) around urban boundaries. Bobcats captured near urban areas had increased exposure to T. gondii in Florida, suggesting an urban source of prey. Bobcats captured near urban areas in Colorado and Florida had higher FIV exposure, possibly suggesting increased intraspecific interactions through pile-up of home ranges. Beyond these regional and pathogen specific relationships, proximity to the wildland-urban interface did not generally increase the probability of disease exposure in wild or domestic felids, emphasizing the importance of local ecological determinants. Indeed, pathogen exposure was often negatively associated with the wildland-urban interface for all felids. Our analyses suggest cross-species pathogen transmission events around this interface may be infrequent, but followed by self-sustaining propagation within the new host species. C1 [Carver, Scott; VandeWoude, Sue] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Carver, Scott; Lunn, Tamika] Univ Tasmania, Sch Biol Sci, Hobart, Tas 7005, Australia. [Bevins, Sarah N.] USDA, Natl Wildlife Dis Program, Ft Collins, CO 80526 USA. [Lappin, Michael R.] Colorado State Univ, Dept Clin Sci, Ft Collins, CO 80523 USA. [Boydston, Erin E.; Lyren, Lisa M.] US Geol Survey, Western Ecol Res Ctr, Thousand Oaks, CA 91360 USA. [Alldredge, Mathew] Colorado Pk & Wildlife Serv, Ft Collins, CO 80526 USA. [Logan, Kenneth A.; Sweanor, Linda L.] Colorado Pk & Wildlife Serv, Montrose, CO 81410 USA. [Riley, Seth P. D.] Natl Pk Serv, Thousand Oaks, CA 91360 USA. [Serieys, Laurel E. K.] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA. [Fisher, Robert N.] US Geol Survey, Western Ecol Res Ctr, San Diego, CA 92101 USA. [Vickers, T. Winston; Boyce, Walter] Univ Calif Davis, Wildlife Hlth Ctr, Davis, CA 95616 USA. [McBride, Roy] Ranchers Supply, Ochopee, FL 34141 USA. [Cunningham, Mark C.] Florida Fish & Wildlife Conservat Commiss, Gainesville, FL 32601 USA. [Jennings, Megan] San Diego State Univ, Inst Ecol Monitoring & Management, San Diego, CA 92182 USA. [Lewis, Jesse; Crooks, Kevin R.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. RP Carver, S (reprint author), Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA.; Carver, S (reprint author), Univ Tasmania, Sch Biol Sci, Hobart, Tas 7005, Australia. EM scott.carver@utas.edu.au FU National Science Foundation Ecology of Infectious Disease Research Program (NSF) [EF-0723676, 1413925]; University of Tasmania; Colorado Parks and Wildlife; U.S. Geological Survey; Florida Fish and Wildlife Conservation Commission; National Park Service; California Department of Fish and Wildlife; California State Parks; Nature Conservancy; Anza Borrego Foundation FX This study was supported by the National Science Foundation Ecology of Infectious Disease Research Program (NSF EF-0723676 and 1413925), and writing of the manuscript was facilitated by a University of Tasmania Visiting Scholarship to Sue VandeWoude. We thank K. Lafferty and two anonymous reviewers for comments and suggestions that helped improve this paper. Multiple humane societies and animal care centers helped with domestic cat samples, including the Montrose Animal Shelter, Ventura County Animal Shelter, Corona Animal Shelter, Escondido Animal Shelter, Pinegate Veterinary Center, Feral Cat Coalition, Boulder Humane Society, and Second Chance Humane Society. We acknowledge A. Griffith, M. Brewer, J. Hawley, A. Morris, V. Scorza, J. Bauer, E. York, M. Ehlbroch, M. Puzzo, G. Lee, and B. Millsap for assistance with sample collection and processing. We also thank Colorado Parks and Wildlife, U.S. Geological Survey, Florida Fish and Wildlife Conservation Commission, National Park Service, California Department of Fish and Wildlife, California State Parks, The Nature Conservancy, and The Anza Borrego Foundation for their collaboration and/or financial support for puma and bobcat capture and sampling. Any use of trade, product, or firm names is for descriptive purposes only and does not imply an endorsement by the U.S. Government. NR 89 TC 3 Z9 3 U1 18 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD MAR PY 2016 VL 26 IS 2 BP 367 EP 381 DI 10.1890/15-0445.1 PG 15 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DJ3PU UT WOS:000374118700004 PM 27209780 ER PT J AU Hurteau, MD Liang, S Martin, KL North, MP Koch, GW Hungate, BA AF Hurteau, Matthew D. Liang, Shuang Martin, Katherine L. North, Malcolm P. Koch, George W. Hungate, Bruce A. TI Restoring forest structure and process stabilizes forest carbon in wildfire-prone southwestern ponderosa pine forests SO ECOLOGICAL APPLICATIONS LA English DT Article DE climate change mitigation; forest carbon; forest restoration; LANDIS-II; ponderosa pine; wildfire ID FUEL-REDUCTION TREATMENTS; STAND-REPLACING FIRE; MIXED-CONIFER FOREST; SIERRA-NEVADA; CLIMATE-CHANGE; UNITED-STATES; NORTHERN ARIZONA; PRESCRIBED FIRE; WATER FLUXES; WESTERN US AB Changing climate and a legacy of fire-exclusion have increased the probability of high-severity wildfire, leading to an increased risk of forest carbon loss in ponderosa pine forests in the southwestern USA. Efforts to reduce high-severity fire risk through forest thinning and prescribed burning require both the removal and emission of carbon from these forests, and any potential carbon benefits from treatment may depend on the occurrence of wildfire. We sought to determine how forest treatments alter the effects of stochastic wildfire events on the forest carbon balance. We modeled three treatments (control, thin-only, and thin and burn) with and without the occurrence of wildfire. We evaluated how two different probabilities of wildfire occurrence, 1% and 2% per year, might alter the carbon balance of treatments. In the absence of wildfire, we found that thinning and burning treatments initially reduced total ecosystem carbon (TEC) and increased net ecosystem carbon balance (NECB). In the presence of wildfire, the thin and burn treatment TEC surpassed that of the control in year 40 at 2%/ yr wildfire probability, and in year 51 at 1%/ yr wildfire probability. NECB in the presence of wildfire showed a similar response to the no-wildfire scenarios: both thin-only and thin and burn treatments increased the C sink. Treatments increased TEC by reducing both mean wildfire severity and its variability. While the carbon balance of treatments may differ in more productive forest types, the carbon balance benefits from restoring forest structure and fire in southwestern ponderosa pine forests are clear. C1 [Hurteau, Matthew D.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Liang, Shuang] Penn State Univ, Intercoll Grad Degree Program Ecol, University Pk, PA 16802 USA. [Liang, Shuang] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Martin, Katherine L.] USDA, US Forest Serv, Ctr Integrated Forest Sci, Southern Res Stn, Res Triangle Pk, NC 27709 USA. [North, Malcolm P.] USDA, US Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Koch, George W.; Hungate, Bruce A.] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Koch, George W.; Hungate, Bruce A.] No Arizona Univ, Dept Biol Sci, Box 5640, Flagstaff, AZ 86011 USA. RP Hurteau, MD (reprint author), Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. EM mhurteau@unm.edu RI Hurteau, Matthew/D-2301-2009 OI Hurteau, Matthew/0000-0001-8457-8974 FU U.S. Department of Defense's Strategic Environmental Research and Development Program (SERDP) [RC-2118] FX Funding for this research was provided by the U.S. Department of Defense's Strategic Environmental Research and Development Program (SERDP, Project RC-2118). We thank the Camp Navajo field crew for assistance with data collection and Bruce Buttrey and Hannah Telle for providing data and facilitating access to the field site. We thank two anonymous reviewers for helpful feedback. NR 71 TC 4 Z9 4 U1 12 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD MAR PY 2016 VL 26 IS 2 BP 382 EP 391 DI 10.1890/15-0337.1 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DJ3PU UT WOS:000374118700005 PM 27209781 ER PT J AU Kochian, LV AF Kochian, Leon V. TI Root architecture SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Editorial Material ID PHOSPHORUS ACQUISITION; EFFICIENCY; CROPS; RICE C1 [Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Kochian, LV (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. NR 20 TC 2 Z9 2 U1 8 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD MAR PY 2016 VL 58 IS 3 SI SI BP 190 EP 192 DI 10.1111/jipb.12471 PG 3 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DJ3XW UT WOS:000374141000001 PM 26915932 ER PT J AU Pineros, MA Larson, BG Shaff, JE Schneider, DJ Falcao, AX Yuan, LX Clark, RT Craft, EJ Davis, TW Pradier, PL Shaw, NM Assaranurak, I McCouch, SR Sturrock, C Bennett, M Kochian, LV AF Pineros, Miguel A. Larson, Brandon G. Shaff, Jon E. Schneider, David J. Falcao, Alexandre Xavier Yuan, Lixing Clark, Randy T. Craft, Eric J. Davis, Tyler W. Pradier, Pierre-Luc Shaw, Nathanael M. Assaranurak, Ithipong McCouch, Susan R. Sturrock, Craig Bennett, Malcolm Kochian, Leon V. TI Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Article DE Abiotic stress; digital root phenotyping; mineral nutrition; root system architecture ID GENETIC-IMPROVEMENT; OPPORTUNITIES; SOIL AB A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture (RSA), the three-dimensional distribution of the different root types in the soil. The ability to image, track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying RSA. We have designed a hydroponic growth system which retains the three-dimensional RSA of the plant root system, while allowing for aeration, solution replenishment and the imposition of nutrient treatments, as well as high-quality imaging of the root system. The simplicity and flexibility of the system allows for modifications tailored to the RSA of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity (detection of fine roots and other root details), higher efficiency, and a broad array of growing conditions for plants that more closely mimic those found under field conditions. C1 [Pineros, Miguel A.; Larson, Brandon G.; Shaff, Jon E.; Schneider, David J.; Clark, Randy T.; Craft, Eric J.; Davis, Tyler W.; Pradier, Pierre-Luc; Shaw, Nathanael M.; Kochian, Leon V.] USDA ARS, Robert Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14580 USA. [Falcao, Alexandre Xavier] Univ Estadual Campinas, Inst Comp, Dept Informat Syst, Av Albert Einstein 1251, BR-13083852 Campinas, SP, Brazil. [Yuan, Lixing] China Agr Univ, Dept Plant Nutr, Beijing 100193, Peoples R China. [Assaranurak, Ithipong; McCouch, Susan R.] Cornell Univ, Dept Genet & Plant Breeding, Ithaca, NY 14853 USA. [Sturrock, Craig; Bennett, Malcolm] Univ Nottingham, Sch Biosci, Ctr Plant Integrat Biol, Loughborough LE12 5RD, Leics, England. [Bennett, Malcolm] King Saud Univ, Coll Sci, Riyadh 11451, Saudi Arabia. RP Kochian, LV (reprint author), USDA ARS, Robert Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14580 USA. EM lvk1@cornell.edu RI Yuan, Lixing/I-2727-2012; Falcao, Alexandre/F-8361-2012; OI Yuan, Lixing/0000-0003-0233-6184; Schneider, David/0000-0002-2124-8385; Pineros, Miguel/0000-0002-7166-1848 FU Biotechnology and Biological Sciences Research Council; Engineering and Physical Sciences Research Council; European Research Council; King Saud University FX C.J.S. and M.J.B. acknowledge the support of the Biotechnology and Biological Sciences Research Council and Engineering and Physical Sciences Research Council funding to the Centre for Plant Integrative Biology. We also acknowledge funding in the form of a Biotechnology and Biological Sciences Research Council Professorial Research Fellowship (to M.J.B.); European Research Council Advanced Investigator Grant funding (FUTUREROOTS) (to M.J.B); and the Distinguished Scientist Fellowship Program (DSFP) at King Saud University to M.J.B. NR 23 TC 4 Z9 4 U1 10 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD MAR PY 2016 VL 58 IS 3 SI SI BP 230 EP 241 DI 10.1111/jipb.12456 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DJ3XW UT WOS:000374141000006 PM 26683583 ER PT J AU Simone-Finstrom, M Walz, M Tarpy, DR AF Simone-Finstrom, Michael Walz, Megan Tarpy, David R. TI Genetic diversity confers colony-level benefits due to individual immunity SO BIOLOGY LETTERS LA English DT Article DE Apis mellifera; polyandry; social insects; individual versus social immunity ID BEES APIS-MELLIFERA; HONEY-BEES; POLYANDRY; EVOLUTION; DISEASE; INFECTIONS; RESISTANCE; SOCIETIES; VARIANCE AB Several costs and benefits arise as a consequence of eusociality and group-living. With increasing group size, spread of disease among nest-mates poses selective pressure on both individual immunity and group-level mechanisms of disease resistance (social immunity). Another factor known to influence colony-level expression of disease is intracolony genetic diversity, which in honeybees (Apis mellifera) is a direct function of the number of mates of the queen. Colonies headed by queens with higher mating numbers have less variable infections of decreased intensity, though the underlying mechanisms remain unclear. By pathogen-challenging larvae in vitro, we decoupled larval immune response from mechanisms of social immunity. Our results show that baseline immunity and degree of immune response do not vary with genetic diversity. However, intracolony variance in antimicrobial peptide production after pathogen challenge decreases with increasing genetic diversity. This reduction in variability of the larval immune response could drive the mitigation of disease observed in genetically diverse colonies. C1 [Simone-Finstrom, Michael; Walz, Megan; Tarpy, David R.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Simone-Finstrom, Michael; Tarpy, David R.] N Carolina State Univ, WM Keck Ctr Behav Biol, Raleigh, NC 27695 USA. [Simone-Finstrom, Michael] USDA ARS, Honey Bee Breeding Genet & Physiol Res, Baton Rouge, LA 70820 USA. RP Simone-Finstrom, M (reprint author), N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. EM michael.simonefinstrom@ars.usda.gov OI Simone-Finstrom, Michael/0000-0003-2938-9788 FU USDA FX Funding provided by a USDA Postdoctoral Fellowship to M.S.-F. NR 25 TC 0 Z9 0 U1 10 U2 17 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1744-9561 EI 1744-957X J9 BIOL LETTERS JI Biol. Lett. PD MAR 1 PY 2016 VL 12 IS 3 AR 20151007 DI 10.1098/rsbl.2015.1007 PG 4 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DJ1AE UT WOS:000373934700006 PM 26961896 ER PT J AU Shapiro, LR Scully, ED Straub, TJ Park, J Stephenson, AG Beattie, GA Gleason, ML Kolter, R Coelho, MC De Moraes, CM Mescher, MC Zhaxybayeva, O AF Shapiro, Lori R. Scully, Erin D. Straub, Timothy J. Park, Jihye Stephenson, Andrew G. Beattie, Gwyn A. Gleason, Mark L. Kolter, Roberto Coelho, Miguel C. De Moraes, Consuelo M. Mescher, Mark C. Zhaxybayeva, Olga TI Horizontal Gene Acquisitions, Mobile Element Proliferation, and Genome Decay in the Host-Restricted Plant Pathogen Erwinia Tracheiphila SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE Cucurbita; Cucumis; Erwinia; mobile DNA; transposase; insertion sequence; pseudogene; host specialization; vector; monomorphic; phage; pumpkin; squash; cucumber ID MONOMORPHIC BACTERIAL PATHOGENS; SODALIS-GLOSSINIDIUS; CUCURBITA-PEPO; SEARCH TOOL; EVOLUTION; SEQUENCE; INSIGHTS; ALIGNMENT; IDENTIFICATION; DOMESTICATION AB Modern industrial agriculture depends on high-density cultivation of genetically similar crop plants, creating favorable conditions for the emergence of novel pathogens with increased fitness in managed compared with ecologically intact settings. Here, we present the genome sequence of six strains of the cucurbit bacterial wilt pathogen Erwinia tracheiphila (Enterobacteriaceae) isolated from infected squash plants in New York, Pennsylvania, Kentucky, and Michigan. These genomes exhibit a high proportion of recent horizontal gene acquisitions, invasion and remarkable amplification of mobile genetic elements, and pseudogenization of approximately 20% of the coding sequences. These genome attributes indicate that E. tracheiphila recently emerged as a host-restricted pathogen. Furthermore, chromosomal rearrangements associated with phage and transposable element proliferation contribute to substantial differences in gene content and genetic architecture between the six E. tracheiphila strains and other Erwinia species. Together, these data lead us to hypothesize that E. tracheiphila has undergone recent evolution through both genome decay (pseudogenization) and genome expansion (horizontal gene transfer and mobile element amplification). Despite evidence of dramatic genomic changes, the six strains are genetically monomorphic, suggesting a recent population bottleneck and emergence into E. tracheiphila's current ecological niche. C1 [Shapiro, Lori R.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Scully, Erin D.] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Scully, Erin D.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Straub, Timothy J.; Zhaxybayeva, Olga] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Park, Jihye] Penn State Univ, Grad Program Bioinformat & Genom, University Pk, PA 16802 USA. [Stephenson, Andrew G.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Beattie, Gwyn A.; Gleason, Mark L.] Iowa State Univ, Dept Plant Pathol & Microbiol, Iowa City, IA USA. [Kolter, Roberto] Harvard Univ, Sch Med, Dept Microbiol & Immunol, Boston, MA USA. [Coelho, Miguel C.] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA. [De Moraes, Consuelo M.; Mescher, Mark C.] ETH, Dept Environm Syst Sci, Zurich, Switzerland. [Zhaxybayeva, Olga] Dartmouth Coll, Dept Comp Sci, Hanover, NH 03755 USA. [Park, Jihye] Massachusetts Gen Hosp, Dept Pediat, Boston, MA 02114 USA. RP Shapiro, LR (reprint author), Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA.; Zhaxybayeva, O (reprint author), Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA.; Zhaxybayeva, O (reprint author), Dartmouth Coll, Dept Comp Sci, Hanover, NH 03755 USA. EM lori.r.shapiro@gmail.com; olgazh@dartmouth.edu FU United States Department of Agriculture [2008-35302-04577, 2009-33120-20093]; National Science Foundation [DBI-1202736]; Simons Foundation FX Bioinformatic analyses were performed using computing resources available at the Research Computing Odyssey cluster at Harvard University (Cambridge, MA). The authors thank Miruna Sasu and Erika Salaau Rojas for the bacterial isolations; Bob Freeman and Aaron Kitzmiller for outstanding computational support; Qian Lui for DNA extractions; Andrew Murray for critical reading of the manuscript, and sequencing and library preparation advice; Olga Shechvenko at the Delaware Genotyping and Sequencing Center for PacBio library prep, sequencing, and assembly assistance; and Christian Daly and Jennifer Cougat at Harvard's Bauer Core for Illumina library preparation advice and for running the Illumina HiSeq samples. Funding for this research was provided by Grants 2008-35302-04577 and 2009-33120-20093 from the United States Department of Agriculture to A.G.S., C.D.M., M.C.M.; National Science Foundation Graduate Research Fellowship to L.R.S.; National Science Foundation Post Doctoral Research Program in Biology (DBI-1202736) to L.R.S.; and in part by a Simons Investigator award from the Simons Foundation to O.Z. NR 87 TC 0 Z9 0 U1 5 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD MAR PY 2016 VL 8 IS 3 BP 649 EP 664 DI 10.1093/gbe/evw016 PG 16 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DI9RD UT WOS:000373839200016 PM 26992913 ER PT J AU Moore, GG Mack, BM Beltz, SB AF Moore, Geromy G. Mack, Brian M. Beltz, Shannon B. TI Draft Genome Sequences of Two Closely Related Aflatoxigenic Aspergillus Species Obtained from the Ivory Coast SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE Aspergillus ochraceoroseus; Aspergillus rambellii; genome sequence; phylogenomics; mating-type locus ID BIOSYNTHESIS GENE CLUSTERS; OCHRACEOROSEUS; FUMIGATUS; FUNGI; B-1 AB Aspergillus ochraceoroseus and Aspergillus rambellii were isolated from soil detritus in Tai National Park, Ivory Coast, Africa. The Type strain for each species happens to be the only representative ever sampled. Both species secrete copious amounts of aflatoxin B-1 and sterigmatocystin, because each of their genomes contains clustered genes for biosynthesis of these mycotoxins. We sequenced their genomes using a personal genome machine and found them to be smaller in size (A. ochraceoroseus = 23.9Mb and A. rambellii = 26.1 Mb), as well as in numbers of predicted genes (7,837 and 7,807, respectively), compared to other sequenced Aspergilli. Our findings also showed that the A. ochraceoroseus Type strain contains a single MAT1-1 gene, while the Type strain of A. rambellii contains a single MAT1-2 gene, indicating that these species are heterothallic (self-infertile). These draft genomes will be useful for understanding the genes and pathways necessary for the cosynthesis of these two toxic secondary metabolites as well as the evolution of these pathways in aflatoxigenic fungi. C1 [Moore, Geromy G.; Mack, Brian M.; Beltz, Shannon B.] ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. RP Moore, GG (reprint author), ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. EM geromy.moore@ars.usda.gov FU Agricultural Research Service, an agency within United States Department of Agriculture FX This work was supported by research funding from the Agricultural Research Service, an agency within the United States Department of Agriculture. NR 18 TC 0 Z9 0 U1 4 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD MAR PY 2016 VL 8 IS 3 BP 729 EP 732 DI 10.1093/gbe/evv246 PG 4 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DI9RD UT WOS:000373839200021 ER PT J AU Huang, YB Lee, MA Thomson, SJ Reddy, KN AF Huang, Yanbo Lee, Matthew A. Thomson, Steven J. Reddy, Krishna N. TI Ground-based hyperspectral remote sensing for weed management in crop production SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE ground-based remote sensing; hyperspectral; crop injury; herbicide resistance; precision agriculture ID GLYPHOSATE; VEGETATION; INJURY; COTTON AB Agricultural remote sensing has been developed and applied in monitoring soil, crop growth, weed infestation, insects, diseases and water status in farm fields to provide data and information to guide agricultural management practices. Precision agriculture has been implemented through prescription mapping of crop fields at different scales with the data remotely sensed from space-borne, airborne and ground-based platforms. Ground-based remote sensing techniques offer portability, flexibility and controllability in applications for precision agriculture. In weed management, crop injury from off-target herbicide spray drift and herbicide resistance in weeds are two important issues. For precision weed management, ground-based hyperspectral remote sensing techniques were developed for detection of crop injury from dicamba and differentiation between glyphosate resistant and sensitive weeds. This research presents the techniques for ground-based hyperspectral remote sensing for these two applications. Results illustrate the advantages of ground-based hyperspectral remote sensing for precision weed management. C1 [Huang, Yanbo; Reddy, Krishna N.] ARS, USDA, Crop Prod Syst Res Unit, POB 350, Stoneville, MS 38776 USA. [Lee, Matthew A.] Mississippi State Univ, Dept Elect & Comp Engn, Mississippi State, MS 39762 USA. [Thomson, Steven J.] USDA, Natl Inst Food & Agr, Washington, DC 20024 USA. RP Huang, YB (reprint author), POB 350,141 Expt Stn Rd, Stoneville, MS 38776 USA. EM yanbo.huang@ars.usda.gov; lee@ece.msstate.edu; steven.j.thomson@nifa.usda.gov; krishna.reddy@ars.usda.gov NR 25 TC 1 Z9 1 U1 12 U2 27 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD MAR PY 2016 VL 9 IS 2 BP 98 EP 109 DI 10.3965/j.ijabe.20160902.2137 PG 12 WC Agricultural Engineering SC Agriculture GA DJ1MB UT WOS:000373965800011 ER PT J AU Dunlap, CA Kim, SJ Kwon, SW Rooney, AP AF Dunlap, Christopher A. Kim, Soo-Jin Kwon, Soon-Wo Rooney, Alejandro P. TI Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens; Bacillus methylotrophicus, Bacillus amyloliquefaciens subsp plantarum and 'Bacillus oryzicola' are later heterotypic synonyms of Bacillus velezensis based on phylogenomics SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID DNA-DNA HYBRIDIZATION; ANALYSIS SHOWS; SP NOV.; RICE AB Bacillus velezensis was previously reported to be a later heterotypic synonym of Bacillus amyloliquefaciens, based primarily on DNA-DNA relatedness values. We have sequenced a draft genome of B. velezensis NRRL B-41580(T). Comparative genomics and DNA-DNA relatedness calculations show that it is not a synonym of B. amyloliquefaciens. It was instead synonymous with Bacillus methylotrophicus. 'Bacillus oryzicola' is a recently described species that was isolated as an endophyte of rice (Oryza sativa). The strain was demonstrated to have plant-pathogen antagonist activity in greenhouse assays, and the 16S rRNA gene was reported to have 99.7 % sequence similarity with Bacillus siamensis and B. methylotrophicus, which are both known for their plant pathogen antagonism. To better understand the phylogenetics of these closely related strains, we sequenced the genome of 'B. oryzicola' KACC 18228. Comparative genomic analysis showed only minor differences between this strain and the genomes of B. velezensis NRRL B-41580(T), B. methylotrophicus KACC 13015(T) and Bacillus amyloliquefaciens subsp. plantarum FZB42(T). The pairwise in silico DNA-DNA hybridization values calculated in comparisons between the strains were all greater than 84 %, which is well above the standard species threshold of 70 %. The results of morphological, physiological, chemotaxonomic and phylogenetic analyses indicate that the strains share phenotype and genotype coherence. Therefore, we propose that B. methylotrophicus KACC 13015(T), B. amyloliquefaciens subsp. plantarum FZB42(T), and 'B. oryzicola' KACC 18228 should be reclassified as later heterotypic synonyms of B. velezensis NRRL B-41580(T), since the valid publication date of B. velezensis precedes the other three strains. C1 [Dunlap, Christopher A.; Kim, Soo-Jin; Rooney, Alejandro P.] ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. [Kim, Soo-Jin; Kwon, Soon-Wo] KACC, Natl Inst Agr Sci, Rural Dev Adm, Jeollabuk Do, South Korea. RP Dunlap, CA (reprint author), ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. EM christopher.dunlap@ars.usda.gov FU 'Research Program for Agricultural Science & Technology Development' of the National Institute of Agricultural Science, Rural Development Administration, Republic of Korea [PJ011248] FX This study was supported by the 'Research Program for Agricultural Science & Technology Development (project no. PJ011248)' of the National Institute of Agricultural Science, Rural Development Administration, Republic of Korea. The authors would like to thank Heather Walker of the National Center for Agricultural Utilization Research, Peoria, IL, USA for expert technical assistance. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. NR 18 TC 7 Z9 7 U1 9 U2 15 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD MAR PY 2016 VL 66 BP 1212 EP 1217 DI 10.1099/ijsem.0.000858 PN 3 PG 6 WC Microbiology SC Microbiology GA DJ3JX UT WOS:000374102200017 ER PT J AU Walsh, GC Dimeglio, AS Khrimian, A Weber, DC AF Cabrera Walsh, Guillermo Dimeglio, Anthony S. Khrimian, Ashot Weber, Donald C. TI Marking and retention of harlequin bug, Murgantia histrionica (Hahn) (Hemiptera: Pentatomidae), on pheromone-baited and unbaited plants SO JOURNAL OF PEST SCIENCE LA English DT Article DE 10,11-epoxy-1-bisabolen-3-ol; Mark-release-recapture; Trap crops; Migration rates; Stink bug ID AGGREGATION PHEROMONE; RANGE AB Harlequin bug (Murgantia histrionica) is an important pest of cole crops in the USA. The adults and nymphs feed on aboveground plant tissues by sucking cell contents and can seriously damage the host. Current insect control measures on cole crops target mainly lepidopteran pests, and the insecticides generally used do not control harlequin bug, so alternative management practices need to be explored. Previous research has established the existence of a male-produced pheromone attractive to both sexes and nymphs of M. histrionica. In this work, two systems of marking bugs were tested to verify if the mark affected fitness traits such as survival and host location. In a second phase, marked individuals were placed on trap host plants baited with synthetic pheromone lures to test whether migration rates were related to M. histrionica density on the trap plants and the presence of the attractants. Neither marking system affected the survival or orientation of the subjects compared to unmarked individuals. The pheromone lures significantly increased the attractiveness of the trap plants, but did not increase the retention time of the plants compared to unbaited plants. Emigration from the trap plants showed a constant rate and seemed unrelated to bug density on the plants. However, a mean peak density of ca. 36 bugs/plant was calculated. Beyond this number, density tended to decrease. These successful marking methods and retention time models support development of M. histrionica management with trap crops, by providing tentative control thresholds and decision rules. C1 [Cabrera Walsh, Guillermo] FUEDEI Invas Species Res Fdn, Bolivar 1559,B1686EFA Hurlingham, Buenos Aires, DF, Argentina. [Dimeglio, Anthony S.; Khrimian, Ashot; Weber, Donald C.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD USA. RP Walsh, GC (reprint author), FUEDEI Invas Species Res Fdn, Bolivar 1559,B1686EFA Hurlingham, Buenos Aires, DF, Argentina. EM gcabrera@fuedei.org NR 26 TC 1 Z9 1 U1 12 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD MAR PY 2016 VL 89 IS 1 BP 21 EP 29 DI 10.1007/s10340-015-0663-1 PG 9 WC Entomology SC Entomology GA DJ4QO UT WOS:000374192400003 ER PT J AU Morrison, WR Lee, DH Short, BD Khrimian, A Leskey, TC AF Morrison, William R., III Lee, Doo-Hyung Short, Brent D. Khrimian, Ashot Leskey, Tracy C. TI Establishing the behavioral basis for an attract-and-kill strategy to manage the invasive Halyomorpha halys in apple orchards SO JOURNAL OF PEST SCIENCE LA English DT Article DE Integrated pest management; Brown marmorated stink bug; Behaviorally based management; Harmonic radar; Invasive species; Pheromone; Hemiptera; Pentatomidae ID MARMORATED STINK BUG; HEMIPTERA-PENTATOMIDAE ANALYSIS; RADAR TAG ATTACHMENT; AGGREGATION PHEROMONE; STAL HETEROPTERA; UNITED-STATES; PLUM CURCULIO; IMPACT; TRAPS; RESPONSES AB Halyomorpha halys (Stal), the brown marmorated stink bug, is an invasive, polyphagous insect that causes serious economic injury in particular to specialty crops in the United States. Growers have been forced to respond by increasing the frequency of broad-spectrum insecticide (e.g., neonicotinoid, pyrethroid, and carbamate) applications. One strategy to reduce reliance on insecticides is known as "attract-and-kill'' whereby the targeted insect is attracted to a spatially precise location to be eliminated by a killing agent such as an insecticide. This approach can substantially reduce the amount of insecticide used by sparing alternate row middle or whole block sprays. For apple orchards, we propose baiting select border row trees with the H. halys aggregation pheromone and synergist and subsequently treating these baited trees with effective insecticides to kill H. halys throughout the growing season. To evaluate the behavioral basis of this approach, we conducted orchard trials with black pyramid traps, harmonic radar, and trials using baited apple trees sprayed weekly to quantify H. halys arrestment area, retention time, adult and nymph annihilation, and fruit injury in and near these attract-and-kill sites. The arrestment area for H. halys was confined to a 2.5 m radius around the pheromone-and pheromone synergist-baited trap regardless of pheromone dose (84 or 840 mg), while the retention capacity of adults was significantly increased by pairing the aggregation pheromone and synergist with a fruiting host plant compared with non-host sites. Damage to fruit harvested from baited attract-and-kill trees was high, but minimal in surrounding unbaited neighboring apple trees. Our results suggest attract-and-kill may be an effective strategy for managing H. halys season-long. C1 [Morrison, William R., III; Short, Brent D.; Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. [Lee, Doo-Hyung] Gachon Univ, Dept Life Sci, Songnam, Gyeonggi Do, South Korea. [Khrimian, Ashot] USDA ARS, NEA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Morrison, WR (reprint author), USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. EM william.morrison@ars.usda.gov OI Morrison, William/0000-0002-1663-8741 FU OREI [2012-51300-20097]; USDA NIFA SCRI [2011-51181-30937] FX We thank John Cullum, Torri Hancock, Brittany Poling, Zach Moore, Nate Brandt, and Matthew Weltz for their excellent technical assistance. This project was funded by OREI #2012-51300-20097 and USDA NIFA SCRI #2011-51181-30937. Mention of trade names or commercial products in this publication is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 52 TC 7 Z9 7 U1 10 U2 24 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD MAR PY 2016 VL 89 IS 1 BP 81 EP 96 DI 10.1007/s10340-015-0679-6 PG 16 WC Entomology SC Entomology GA DJ4QO UT WOS:000374192400008 ER PT J AU Baldwin, RA Meinerz, R Witmer, GW AF Baldwin, Roger A. Meinerz, Ryan Witmer, Gary W. TI Cholecalciferol plus diphacinone baits for vole control: a novel approach to a historic problem SO JOURNAL OF PEST SCIENCE LA English DT Article DE Artichoke; California vole; Cholecalciferol; Diphacinone; Microtus californicus; Resistance ID MICROTUS-CALIFORNICUS; POPULATIONS; PATTERNS AB Combination baits containing cholecalciferol plus an anticoagulant are effective against commensal rodents resistant to anticoagulants, and they likely pose less risk than anticoagulant-only rodenticides due to lower concentrations of active ingredients and shorter time to death. However, these combination baits have not been tested for agricultural rodent pests. Therefore, we established a study to test the efficacy of cholecalciferol plus diphacinone artichoke bract and pellet baits to determine their ability to manage California voles Microtus californicus in artichokes, where resistance to anticoagulants is known to occur. Field tests using radiocollared voles indicated that bract baits were highly efficacious (85 %), although pellet baits were less effective (60 %). Low observed efficacy of pellet baits may have resulted from poor weather following application during the second sampling period; further testing may yield more positive results. We observed a bimodal distribution in timing of death, with one group of voles dying between 4.3 and 5.8 days post-consumption; the other group died between 9.0 and 14.5 days post-consumption. Deaths in the first group were attributed to cholecalciferol, while deaths in the second group were likely due to chronic anticoagulant exposure. Almost double the proportion of voles that died from bract consumption did so during the early period when compared to their pellet plot counterparts. This suggests that voles were consuming greater quantities of bract baits over a shorter period of time when compared to the pellet bait. Collectively, these findings indicate that baiting with cholecalciferol plus diphacinone coated bracts is an effective method for controlling vole populations in artichokes. C1 [Baldwin, Roger A.; Meinerz, Ryan] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, One Shields Ave, Davis, CA 95616 USA. [Witmer, Gary W.] Natl Wildlife Res Ctr, Wildlife Serv, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Baldwin, RA (reprint author), Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, One Shields Ave, Davis, CA 95616 USA. EM rabaldwin@ucdavis.edu FU Vertebrate Pest Control Research Advisory Committee of the California Department of Food and Agriculture FX We thank Ocean Mist and Sea Mist Farms for all of the assistance and resources they have provided throughout the duration of this project. In particular, we thank F. Castaneda, C. Drew, D. Huss, and their highly efficient staff for the tremendous effort they have dedicated to this project. We also greatly appreciate the insight and guidance provided by C. Eason and D. MacMorran on the use of C+D baits and for formulating the products for our use. T. Salmon provided historical guidance on bait mixing. This project was funded by the Vertebrate Pest Control Research Advisory Committee of the California Department of Food and Agriculture. NR 31 TC 2 Z9 2 U1 0 U2 1 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD MAR PY 2016 VL 89 IS 1 BP 129 EP 135 DI 10.1007/s10340-015-0653-3 PG 7 WC Entomology SC Entomology GA DJ4QO UT WOS:000374192400012 ER PT J AU Eberle, KC McGill, JL Reinhardt, TA Sacco, RE AF Eberle, Kirsten C. McGill, Jodi L. Reinhardt, Timothy A. Sacco, Randy E. TI Parainfluenza Virus 3 Blocks Antiviral Mediators Downstream of the Interferon Lambda Receptor by Modulating Stat1 Phosphorylation SO JOURNAL OF VIROLOGY LA English DT Article ID RESPIRATORY SYNCYTIAL VIRUS; PARAMYXOVIRUS V-PROTEINS; SERINE PHOSPHORYLATION; EPITHELIAL-CELLS; GENE-EXPRESSION; III INTERFERON; ALPHA-INTERFERON; RNA HELICASE; IFN-LAMBDA; RIG-I AB Parainfluenza viruses are known to inhibit type I interferon (IFN) production; however, there is a lack of information regarding the type III IFN response during infection. Type III IFNs signal through a unique heterodimeric receptor, IFN-lambda R1/interleukin10R2 (IL-10R2), which is primarily expressed by epithelial cells. Parainfluenza virus 3 (PIV-3) infection is highly restricted to the airway epithelium. We therefore sought to examine type III IFN signaling pathways during PIV-3 infection of epithelial cells. We used three strains of PIV-3: human PIV-3 (HPIV-3), bovine PIV-3 (BPIV-3), and dolphin PIV-1 (Tursiops truncatus PIV-1, or TtPIV-1). Here, we show that message levels of IL-29 are significantly increased during PIV-3 infection, yet downstream antiviral signaling molecules are not upregulated to levels similar to those of the positive control. Furthermore, in Vero cells infected with PIV-3, stimulation with recombinant IL-29/-28A/-28B does not cause upregulation of downstream antiviral molecules, suggesting that PIV-3 interferes with the JAK/STAT pathway downstream of the IFN-lambda R1/IL-10R2 receptor. We used Western blotting to examine the phosphorylation of Stat1 and Stat2 in Vero cells and the bronchial epithelial cell line BEAS-2B. In Vero cells, we observed reduced phosphorylation of the serine 727 (S727) site on Stat1, while in BEAS-2B cells Stat1 phosphorylation was decreased at the tyrosine 701 (Y701) site during PIV-3 infection. PIV-3 therefore interferes with the phosphorylation of Stat1 downstream of the type III IFN receptor. These data provide new evidence regarding strategies employed by parainfluenza viruses to effectively circumvent respiratory epithelial cell-specific antiviral immunity. C1 [Eberle, Kirsten C.; McGill, Jodi L.; Reinhardt, Timothy A.; Sacco, Randy E.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Eberle, Kirsten C.; Sacco, Randy E.] Iowa State Univ, Mol Cellular & Dev Biol Grad Program, Ames, IA USA. [Eberle, Kirsten C.; Sacco, Randy E.] Iowa State Univ, Immunobiol Grad Program, Ames, IA USA. [McGill, Jodi L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. RP Sacco, RE (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA.; Sacco, RE (reprint author), Iowa State Univ, Mol Cellular & Dev Biol Grad Program, Ames, IA USA.; Sacco, RE (reprint author), Iowa State Univ, Immunobiol Grad Program, Ames, IA USA. EM randy.sacco@ars.usda.gov FU DOD \ Office of Naval Research (ONR) [N0001412IP20029] FX DOD vertical bar Office of Naval Research (ONR) provided funding to Randy E Sacco under grant number N0001412IP20029. NR 50 TC 2 Z9 2 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD MAR PY 2016 VL 90 IS 6 BP 2948 EP 2958 DI 10.1128/JVI.02502-15 PG 11 WC Virology SC Virology GA DJ3MW UT WOS:000374110600021 PM 26719274 ER PT J AU Liu, YL Hancock, M Workman, A Doster, A Jones, C AF Liu, Yilin Hancock, Morgan Workman, Aspen Doster, Alan Jones, Clinton TI beta-Catenin, a Transcription Factor Activated by Canonical Wnt Signaling, Is Expressed in Sensory Neurons of Calves Latently Infected with Bovine Herpesvirus 1 SO JOURNAL OF VIROLOGY LA English DT Article ID SIMPLEX-VIRUS TYPE-1; DEXAMETHASONE-INDUCED REACTIVATION; TRIGEMINAL GANGLIONIC NEURONS; PROGRAMMED CELL-DEATH; PRODUCTIVE INFECTION; DEPENDENT TRANSCRIPTION; REGULATORY PROTEINS; INHIBITS APOPTOSIS; READING FRAME; COLON-CANCER AB Like many Alphaherpesvirinae subfamily members, bovine herpesvirus 1 (BoHV-1) expresses an abundant transcript in latently infected sensory neurons, the latency-related (LR)-RNA. LR-RNA encodes a protein (ORF2) that inhibits apoptosis, interacts with Notch family members, interferes with Notch-mediated transcription, and stimulates neurite formation in cells expressing Notch. An LR mutant virus containing stop codons at the amino terminus of ORF2 does not reactivate from latency or replicate efficiently in certain tissues, indicating that LR gene products are important. In this study, beta-catenin, a transcription factor activated by the canonical Wnt signaling pathway, was frequently detected in ORF2-positive trigeminal ganglionic neurons of latently infected, but not mock-infected, calves. Conversely, the lytic cycle regulatory protein (BoHV-1 infected cell protein 0, or bICP0) was not frequently detected in beta-catenin-positive neurons in latently infected calves. During dexamethasone-induced reactivation from latency, mRNA expression levels of two Wnt antagonists, Dickkopf-1 (DKK-1) and secreted Frizzled-related protein 2 (SFRP2), were induced in bovine trigeminal ganglia (TG), which correlated with reduced beta-catenin protein expression in TG neurons 6 h after dexamethasone treatment. ORF2 and a coactivator of beta catenin, mastermind-like protein 1 (MAML1), stabilized beta catenin protein levels and stimulated beta catenin-dependent transcription in mouse neuroblastoma cells more effectively than MAML1 or ORF2 alone. Neuroblastoma cells expressing ORF2, MAML1, and beta-catenin were highly resistant to cell death following serum withdrawal, whereas most cells transfected with only one of these genes died. The Wnt signaling pathway interferes with neurodegeneration but promotes neuronal differentiation, suggesting that stabilization of beta-catenin expression by ORF2 promotes neuronal survival and differentiation. C1 [Liu, Yilin; Hancock, Morgan; Doster, Alan; Jones, Clinton] Univ Nebraska, Morrison Life Sci Res Ctr, Sch Vet Med & Biomed Sci, Nebraska Ctr Virol, Lincoln, NE USA. [Workman, Aspen] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Jones, Clinton] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA. RP Jones, C (reprint author), Univ Nebraska, Morrison Life Sci Res Ctr, Sch Vet Med & Biomed Sci, Nebraska Ctr Virol, Lincoln, NE USA. EM clint.jones10@okstate.edu FU HHS \ National Institutes of Health (NIH) [1P20RR15635]; U.S. Department of Agriculture (USDA) [13-01041] FX HHS vertical bar National Institutes of Health (NIH) provided funding to Clinton J. Jones under grant number 1P20RR15635. U.S. Department of Agriculture (USDA) provided funding to Clinton J. Jones under grant number 13-01041. NR 91 TC 1 Z9 1 U1 5 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD MAR PY 2016 VL 90 IS 6 BP 3148 EP 3159 DI 10.1128/JVI.02971-15 PG 12 WC Virology SC Virology GA DJ3MW UT WOS:000374110600038 PM 26739046 ER PT J AU Zhang, J Yang, CH Song, HB Hoffmann, WC Zhang, DY Zhang, GZ AF Zhang, Jian Yang, Chenghai Song, Huaibo Hoffmann, Wesley Clint Zhang, Dongyan Zhang, Guozhong TI Evaluation of an Airborne Remote Sensing Platform Consisting of Two Consumer-Grade Cameras for Crop Identification SO REMOTE SENSING LA English DT Article DE consumer-grade camera; pixel-based classification; object-based classification; RGB; near-infrared; crop identification ID UNMANNED AERIAL SYSTEMS; ACCURACY ASSESSMENT; DIGITAL CAMERAS; IMAGE-ANALYSIS; CLASSIFICATION; VEGETATION; INDEX; SOIL; ACQUISITION; AIRCRAFT AB Remote sensing systems based on consumer-grade cameras have been increasingly used in scientific research and remote sensing applications because of their low cost and ease of use. However, the performance of consumer-grade cameras for practical applications has not been well documented in related studies. The objective of this research was to apply three commonly-used classification methods (unsupervised, supervised, and object-based) to three-band imagery with RGB (red, green, and blue bands) and four-band imagery with RGB and near-infrared (NIR) bands to evaluate the performance of a dual-camera imaging system for crop identification. Airborne images were acquired from a cropping area in Texas and mosaicked and georeferenced. The mosaicked imagery was classified using the three classification methods to assess the usefulness of NIR imagery for crop identification and to evaluate performance differences between the object-based and pixel-based methods. Image classification and accuracy assessment showed that the additional NIR band imagery improved crop classification accuracy over the RGB imagery and that the object-based method achieved better results with additional non-spectral image features. The results from this study indicate that the airborne imaging system based on two consumer-grade cameras used in this study can be useful for crop identification and other agricultural applications. C1 [Zhang, Jian] Huazhong Agr Univ, Coll Resource & Environm, 1 Shizishan St, Wuhan 430070, Peoples R China. [Zhang, Jian; Yang, Chenghai; Song, Huaibo; Hoffmann, Wesley Clint; Zhang, Dongyan] USDA ARS, Aerial Applicat Technol Res Unit, 3103 F&B Rd, College Stn, TX 77845 USA. [Song, Huaibo] Northwest A&F Univ, Coll Mech & Elect Engn, 22 Xinong Rd, Yangling 712100, Peoples R China. [Zhang, Dongyan] Anhui Univ, Anhui Engn Lab Agroecol Big Data, 111 Jiulong Rd, Hefei 230601, Peoples R China. [Zhang, Guozhong] Huazhong Agr Univ, Coll Engn, 1 Shizishan St, Wuhan 430070, Peoples R China. RP Yang, CH (reprint author), USDA ARS, Aerial Applicat Technol Res Unit, 3103 F&B Rd, College Stn, TX 77845 USA. EM jz@mail.hzau.edu.cn; chenghai.yang@ars.usda.gov; songyangfeifei@163.com; clint.hoffmann@ars.usda.gov; hello-lion@hotmail.com; zhanggz@mail.hzau.edu.cn OI Zhang, Jian/0000-0001-9890-3598 FU National Natural Science Foundation of China [41201364, 31501222]; China Scholarship Council [201308420447] FX This project was conducted as part of a visiting scholar research program, and the first author was financially supported by the National Natural Science Foundation of China (Grant No. 41201364 and 31501222) and the China Scholarship Council (201308420447). The author wishes to thank Jennifer Marshall and Nicholas Mondrik of the College of Science of Texas A & M University, College Station, Texas, for allowing us to use their monochromator and assisting in the measurements of the spectral response of the cameras. Thanks are also extended to Fred Gomez and Lee Denham of USDA-ARS in College Station, Texas, for acquiring the images for this study. NR 49 TC 0 Z9 0 U1 9 U2 19 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD MAR PY 2016 VL 8 IS 3 DI 10.3390/rs8030257 PG 23 WC Remote Sensing SC Remote Sensing GA DI6RJ UT WOS:000373627400026 ER PT J AU Hartman, GL Pawlowski, ML Herman, TK Eastburn, D AF Hartman, Glen L. Pawlowski, Michelle L. Herman, Theresa K. Eastburn, Darin TI Organically Grown Soybean Production in the USA: Constraints and Management of Pathogens and Insect Pests SO AGRONOMY-BASEL LA English DT Review DE soybean; edamame; soymilk; tofu; aphids; rust; Sclerotinia stem rot; stink bugs; sudden death syndrome; cyst nematode ID PROMOTING RHIZOBACTERIA; SOILBORNE DISEASES; BIOLOGICAL-CONTROL; NORTH-AMERICA; YIELD; AGRICULTURE; TEMPERATURE; CROPS AB Soybean is the most produced and consumed oil seed crop worldwide. In 2013, 226 million metric tons were produced in over 70 countries. Organically produced soybean represents less than 0.1% of total world production. In the USA, the certified organic soybean crop was grown on 53 thousand ha or 0.17% of the total soybean acreage in the USA (32 million ha) in 2011. A gradual increase in production of organically grown soybean has occurred since the inception of organic labeling due to increased human consumption of soy products and increased demand for organic soybean meal to produce organic animal products. Production constraints caused by pathogens and insect pests are often similar in organic and non-organic soybean production, but management between the two systems often differs. In general, the non-organic, grain-type soybean crop are genetically modified higher-yielding cultivars, often with disease and pest resistance, and are grown with the use of synthetic pesticides. The higher value of organically produced soybean makes production of the crop an attractive option to some farmers. This article reviews production and uses of organically grown soybean in the USA, potential constraints to production caused by pathogens and insect pests, and management practices used to reduce the impact of these constraints. C1 [Hartman, Glen L.] USDA ARS, 1101 W Peabody St, Urbana, IL 61801 USA. [Pawlowski, Michelle L.; Herman, Theresa K.; Eastburn, Darin] Univ Illinois, Dept Crop Sci, 1101 W Peabody St, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), USDA ARS, 1101 W Peabody St, Urbana, IL 61801 USA. EM ghartman@illinois.edu; mpawlow4@illinois.edu; therm@illinois.edu; eastburn@illinois.edu NR 63 TC 0 Z9 0 U1 13 U2 27 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD MAR PY 2016 VL 6 IS 1 AR 16 DI 10.3390/agronomy6010016 PG 18 WC Agronomy SC Agriculture GA DI5HR UT WOS:000373530000004 ER PT J AU Klarquist, EF Chen, XM Carter, AH AF Klarquist, Emily F. Chen, Xianming M. Carter, Arron H. TI Novel QTL for Stripe Rust Resistance on Chromosomes 4A and 6B in Soft White Winter Wheat Cultivars SO AGRONOMY-BASEL LA English DT Article DE plant breeding; Puccinia striiformis f; sp; tritici; disease resistance; QTL mapping; Triticum aestivum ID ADULT-PLANT RESISTANCE; MARKER-ASSISTED SELECTION; F-SP TRITICI; PUCCINIA-STRIIFORMIS; HIGH-TEMPERATURE; GENETIC-ANALYSIS; UNITED-STATES; LINKAGE MAPS; IDENTIFICATION; DICOCCOIDES AB Stripe rust (caused by Puccinia striiformis f. sp. tritici) of wheat (Triticum aestivum) is a devastating disease in temperate regions when susceptible varieties are grown and environmental conditions sustain high disease pressures. With frequent and severe outbreaks, disease resistance is a key tool for controlling stripe rust on wheat. The goal of this research was to identify quantitative trait loci (QTL) involved in stripe rust resistance from the important US Pacific Northwest soft white winter wheat varieties Eltan and Finch. An F-2:5 recombinant inbred line (RIL) mapping population of 151 individuals derived from the Finch x Eltan cross was developed through single seed descent. A linkage map comprising 683 unique single nucleotide polymorphism (SNP) loci and 70 SSR markers were used to develop 22 linkage groups consisting of 16 of the 21 chromosomes. Stripe rust data were collected on the RILs during the summers of 2012 to 2014. QTL analysis identified two genomic regions on chromosomes 4A (QYrel.wak-4A) and 6B (QYrfi.wak-6B) associated with resistance from Eltan and Finch, respectively. The results of the QTL analysis showed that QYrel.wak-4A and QYrfi.wak-6B reduced infection type and disease severity. Based upon both molecular and phenotypic differences, QYrel.wak-4A is a novel QTL for adult plant resistance (APR) to stripe rust. C1 [Klarquist, Emily F.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Chen, Xianming M.] Washington State Univ, USDA ARS, Wheat Genet Physiol Qual & Dis Res Unit, Pullman, WA 99164 USA. [Chen, Xianming M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Carter, Arron H.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Carter, AH (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM emily.klaruist@wsu.edu; xianming@wsu.edu; ahcarter@wsu.edu NR 50 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD MAR PY 2016 VL 6 IS 1 AR 4 DI 10.3390/agronomy6010004 PG 14 WC Agronomy SC Agriculture GA DI5HR UT WOS:000373530000006 ER PT J AU Fooks, JR Higgins, N Messer, KD Duke, JM Hellerstein, D Lynch, L AF Fooks, Jacob R. Higgins, Nathaniel Messer, Kent D. Duke, Joshua M. Hellerstein, Daniel Lynch, Lori TI CONSERVING SPATIALLY EXPLICIT BENEFITS IN ECOSYSTEM SERVICE MARKETS: EXPERIMENTAL TESTS OF NETWORK BONUSES AND SPATIAL TARGETING SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE Agglomeration; contiguity; ecosystem service markets; experimental economics; network bonuses; reverse auctions; spatial targeting ID CONSERVATION CONTRACTS; PRESERVATION PROGRAMS; HABITAT FRAGMENTATION; INCENTIVES; LANDSCAPE; AUCTIONS; BIODIVERSITY; EFFICIENCY; SCALE AB Conserving contiguous areas often enhances environmental benefits. However, most conservation efforts are incentive-based and voluntary, and neither reward landowners for contiguity nor do they select based on contiguity. Thus, achieving optimal contiguity of conserved parcels is unlikely. Using lab and artefactual field experiments, this paper evaluates two mechanisms in the context of reverse auctions for achieving optimal contiguity: network bonuses and spatial targeting. Results suggest that spatial targeting alone improves the aggregate environmental and social welfare outcomes, while network bonuses alone result in worse outcomes. The interaction of the bonus effect and the targeting effect is positive. If a program was already using a competitive auction environment with bonuses, adding spatial targeting could reduce welfare loss. C1 [Fooks, Jacob R.; Higgins, Nathaniel; Hellerstein, Daniel; Lynch, Lori] USDA, Econ Res Serv, Ames, IA USA. [Messer, Kent D.; Duke, Joshua M.] Univ Delaware, Newark, DE 19716 USA. RP Fooks, JR (reprint author), USDA, Econ Res Serv, Ames, IA USA. EM jacob.fooks@ers.usda.gov FU USDA Economic Research Service [58-6000-7-0089] FX Jacob R. Fooks, Nathaniel Higgins, Daniel Hellerstein, and Lori Lynch are agricultural economists with the USDA Economic Research Service. Kent D. Messer and Joshua M. Duke are professors from the University of Delaware. Financial support for this research came from the USDA Economic Research Service (ID#58-6000-7-0089). The views expressed here are those of the authors, and may not be attributed to the Economic Research Service or the U.S. Department of Agriculture. Correspondence may be sent to: jacob.fooks@ers.usda.gov. NR 41 TC 1 Z9 1 U1 2 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD MAR PY 2016 VL 98 IS 2 BP 468 EP 488 DI 10.1093/ajae/aav061 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DI7WY UT WOS:000373713900008 ER PT J AU Adjemian, MK Saitone, TL Sexton, RJ AF Adjemian, Michael K. Saitone, Tina L. Sexton, Richard J. TI A FRAMEWORK TO ANALYZE THE PERFORMANCE OF THINLY TRADED AGRICULTURAL COMMODITY MARKETS SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE modern agricultural markets; thin markets ID COUNTERVAILING POWER; INCENTIVES; PRICES; RUN AB Thinly traded agricultural commodity markets are a concern for farmers and policy markers due to the belief that prices in these settings will be highly volatile, subject to manipulation, and incapable of efficiently allocating resources. Analysis of thin agricultural markets has to date been impeded by lack of an appropriate analytical framework from which to study their behavior. In this paper we propose the modern agricultural markets (MAM) framework as an appropriate paradigm through which to view and evaluate thin markets. We argue that thinly traded markets that meet key conditions required for a MAM will generate maximum economic surplus and enable farmers to earn at least a competitive return on their investments. In the absence of these conditions, however, the concerns known as the "thin market problem" have validity. We set forth the MAM framework, interpret it in a thin-market context, and conduct several brief case studies of thin markets to illustrate use of the approach and draw some key inferences about these markets' behavior. The analysis indicates that appropriate government policies directed to thin markets are those that facilitate their convergence to MAM status, but in reality key policies under recent consideration would have the opposite effect. C1 [Adjemian, Michael K.] USDA, Econ Res Service, Ames, IA USA. [Saitone, Tina L.; Sexton, Richard J.] Univ Calif Davis, Agr & Resource Econ Dept, Davis, CA USA. RP Sexton, RJ (reprint author), Univ Calif Davis, Agr & Resource Econ Dept, Davis, CA USA. EM rich@primal.ucdavis.edu FU US Department of Agriculture, Economic Research Service [58-3000-3-0052]; University of California, Davis [58-3000-3-0052] FX Michael K. Adjemian is an agricultural economist with the United States Department of Agriculture, Economic Research Service. Tina L. Saitone is a project economist, and Richard J. Sexton is Professor and Chair, in the Agricultural and Resource Economics Department at the University of California, Davis. This research was conducted as part of cooperative research agreement no. 58-3000-3-0052 between the US Department of Agriculture, Economic Research Service, and the University of California, Davis. The views expressed in this article are those of the authors and should not be attributed to USDA or the Economic Research Service. Correspondence may be sent to: rich@primal.ucdavis.edu. NR 50 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD MAR PY 2016 VL 98 IS 2 BP 581 EP 596 DI 10.1093/ajae/aav074 PG 16 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DI7WY UT WOS:000373713900014 ER PT J AU Bowman, M Marshall, KK Kuchler, F Lynch, L AF Bowman, Maria Marshall, Kandice K. Kuchler, Fred Lynch, Lori TI RAISED WITHOUT ANTIBIOTICS: LESSONS FROM VOLUNTARY LABELING OF ANTIBIOTIC USE PRACTICES IN THE BROILER INDUSTRY SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article ID GROWTH-PROMOTING ANTIBIOTICS; WILLINGNESS-TO-PAY; ANTIMICROBIAL RESISTANCE; FOOD ATTRIBUTES; POULTRY LITTER; MARKETS; INFORMATION; CHICKEN; QUALITY; ENTEROCOCCI AB Voluntary labeling improves market efficiency if consumers receive the product characteristics they demand and producers are compensated for producing these characteristics. For this to occur, label claims have to be truthful, credible, and understandable. We examine the introduction of "Raised Without Antibiotics" (RWA) label claims in the broiler industry by Perdue Farms (Perdue) and Tyson Foods (Tyson) in 2007 and the events that follow. We ask whether voluntary label claims were able to improve efficiency in the market for RWA chicken products. In this case, evidence presented as part of court testimony suggests that Tyson did not deliver the RWA attribute that consumers demanded, competitors suffered, and Tyson profited from introducing what was ruled to be a false and misleading label claim to the marketplace. While the media informed consumers and stockholders of these events, we found little evidence in publicly available data, literature, or documentation from court cases that Tyson suffered significant harm in the market, or from stockholders. The U.S. Department of Agriculture Food Safety and Inspection Service did ultimately remove Tyson's label claims from the marketplace, and the legal system provided some redress to competitors and consumers. Even so, labels with animal raising claims (such as RWA) and voluntary, process-based label claims (in general) continue to be challenged by similar issues, that is, consumer confusion, the absence of universally accepted definitions, and varying degrees of credibility. C1 [Bowman, Maria] USDA, Conservat & Environm Branch, Econ Res Serv, ERS USDA, Sunbury, PA 17801 USA. [Marshall, Kandice K.] ERS USDA, Crops Branch, Washington, DC USA. [Kuchler, Fred] ERS USDA, Agr Econ Diet Safety & Hlth Econ Branch, Washington, DC USA. [Lynch, Lori] ERS USDA, Conservat & Environm Branch, Washington, DC USA. RP Bowman, M (reprint author), USDA, Conservat & Environm Branch, Econ Res Serv, ERS USDA, Sunbury, PA 17801 USA. EM maria.bowman@ers.usda.gov NR 104 TC 0 Z9 0 U1 8 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD MAR PY 2016 VL 98 IS 2 BP 622 EP 642 DI 10.1093/ajae/aaw008 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DI7WY UT WOS:000373713900017 ER PT J AU Halofsky, JE Peterson, DL AF Halofsky, Jessica E. Peterson, David L. TI Climate Change Vulnerabilities and Adaptation Options for Forest Vegetation Management in the Northwestern USA SO ATMOSPHERE LA English DT Article DE climate change; vegetation; vulnerability assessment; resource management; adaptation ID PACIFIC-NORTHWEST; UNITED-STATES; ECOSYSTEMS; INCREASE; GROWTH; WILDFIRE; IMPACTS AB Recent vulnerability assessments, conducted in diverse regions in the northwestern United States, indicate that many commonalities exist with respect to projected vulnerabilities to climate change. Dry forests are projected to have significant changes in distribution and abundance of species, partially in response to higher temperature and lower soil moisture, but mostly in response to projected increases in extreme events and disturbances-drought, wildfire, and insect outbreaks. Wildfire and mountain pine beetles have caused extensive mortality across millions of hectares in this region during the past decade, and wildfire area burned is projected to increase 200%-300% by mid-21st century. Science-management partnerships associated with recent assessments have identified an extensive list of adaptation options, including both strategies (general planning) and tactics (on-the-ground projects). Most of the options focus on increasing resilience to disturbances and on reducing current stressors to resource conditions. Adaptation options are generally similar across the biogeographically diverse region covered by assessments, suggesting that there may be a limit on the number of feasible responses to climate change. Federal agencies in the northwestern United States are now using these assessments and adaptation approaches to inform sustainable resource management and planning, mostly through fine tuning of existing practices and policies. C1 [Halofsky, Jessica E.] Univ Washington, Sch Environm & Forest Sci, POB 352100, Seattle, WA 98195 USA. [Peterson, David L.] US Forest Serv, Pacific NW Res Stn, 400 N 34th St, Seattle, WA 98103 USA. RP Halofsky, JE (reprint author), Univ Washington, Sch Environm & Forest Sci, POB 352100, Seattle, WA 98195 USA. EM jhalo@uw.edu; peterson@fs.fed.us FU U.S. Forest Service Office of Sustainability and Climate Change; Pacific Northwest Research Station FX This study was supported by funding from the U.S. Forest Service Office of Sustainability and Climate Change and Pacific Northwest Research Station. This is a contribution of the Western Mountain Initiative. NR 49 TC 1 Z9 1 U1 5 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4433 J9 ATMOSPHERE-BASEL JI Atmosphere PD MAR PY 2016 VL 7 IS 3 DI 10.3390/atmos7030046 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DI5HO UT WOS:000373529700009 ER PT J AU Abbott, BW Jones, JB Schuur, EAG Chapin, FS Bowden, WB Bret-Harte, MS Epstein, HE Flannigan, MD Harms, TK Hollingsworth, TN Mack, MC McGuire, AD Natali, SM Rocha, AV Tank, SE Turetsky, MR Vonk, JE Wickland, KP Aiken, GR Alexander, HD Amon, RMW Benscoter, BW Bergeron, Y Bishop, K Blarquez, O Bond-Lamberty, B Breen, AL Buffam, I Cai, YH Carcaillet, C Carey, SK Chen, JM Chen, HYH Christensen, TR Cooper, LW Cornelissen, JHC de Groot, WJ DeLuca, TH Dorrepaal, E Fetcher, N Finlay, JC Forbes, BC French, NHF Gauthier, S Girardin, MP Goetz, SJ Goldammer, JG Gough, L Grogan, P Guo, LD Higuera, PE Hinzman, L Hu, FS Hugelius, G Jafarov, EE Jandt, R Johnstone, JF Karlsson, J Kasischke, ES Kattner, G Kelly, R Keuper, F Kling, GW Kortelainen, P Kouki, J Kuhry, P Laudon, H Laurion, I Macdonald, RW Mann, PJ Martikainen, PJ McClelland, JW Molau, U Oberbauer, SF Olefeldt, D Pare, D Parisien, MA Payette, S Peng, CH Pokrovsky, OS Rastetter, EB Raymond, PA Raynolds, MK Rein, G Reynolds, JF Robards, M Rogers, BM Schadel, C Schaefer, K Schmidt, IK Shvidenko, A Sky, J Spencer, RGM Starr, G Striegl, RG Teisserenc, R Tranvik, LJ Virtanen, T Welker, JM Zimov, S AF Abbott, Benjamin W. Jones, Jeremy B. Schuur, Edward A. G. Chapin, F. Stuart, III Bowden, William B. Bret-Harte, M. Syndonia Epstein, Howard E. Flannigan, Michael D. Harms, Tamara K. Hollingsworth, Teresa N. Mack, Michelle C. McGuire, A. David Natali, Susan M. Rocha, Adrian V. Tank, Suzanne E. Turetsky, Merritt R. Vonk, Jorien E. Wickland, Kimberly P. Aiken, George R. Alexander, Heather D. Amon, Rainer M. W. Benscoter, Brian W. Bergeron, Yves Bishop, Kevin Blarquez, Olivier Bond-Lamberty, Ben Breen, Amy L. Buffam, Ishi Cai, Yihua Carcaillet, Christopher Carey, Sean K. Chen, Jing M. Chen, Han Y. H. Christensen, Torben R. Cooper, Lee W. Cornelissen, J. Hans C. de Groot, William J. DeLuca, Thomas H. Dorrepaal, Ellen Fetcher, Ned Finlay, Jacques C. Forbes, Bruce C. French, Nancy H. F. Gauthier, Sylvie Girardin, Martin P. Goetz, Scott J. Goldammer, Johann G. Gough, Laura Grogan, Paul Guo, Laodong Higuera, Philip E. Hinzman, Larry Hu, Feng Sheng Hugelius, Gustaf Jafarov, Elchin E. Jandt, Randi Johnstone, Jill F. Karlsson, Jan Kasischke, Eric S. Kattner, Gerhard Kelly, Ryan Keuper, Frida Kling, George W. Kortelainen, Pirkko Kouki, Jari Kuhry, Peter Laudon, Hjalmar Laurion, Isabelle Macdonald, Robie W. Mann, Paul J. Martikainen, Pertti J. McClelland, James W. Molau, Ulf Oberbauer, Steven F. Olefeldt, David Pare, David Parisien, Marc-Andre Payette, Serge Peng, Changhui Pokrovsky, Oleg S. Rastetter, Edward B. Raymond, Peter A. Raynolds, Martha K. Rein, Guillermo Reynolds, James F. Robards, Martin Rogers, Brendan M. Schaedel, Christina Schaefer, Kevin Schmidt, Inger K. Shvidenko, Anatoly Sky, Jasper Spencer, Robert G. M. Starr, Gregory Striegl, Robert G. Teisserenc, Roman Tranvik, Lars J. Virtanen, Tarmo Welker, Jeffrey M. Zimov, Sergei TI Biomass offsets little or none of permafrost carbon release from soils, streams, and wildfire: an expert assessment SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE permafrost carbon; Arctic; boreal; wildfire; dissolved organic carbon; particulate organic carbon; coastal erosion ID CLIMATE-CHANGE; BOREAL FOREST; NITROGEN DEPOSITION; INTERIOR ALASKA; ARCTIC TUNDRA; FIRE; SEQUESTRATION; VULNERABILITY; ECOSYSTEMS; STORAGE AB As the permafrost region warms, its large organic carbon pool will be increasingly vulnerable to decomposition, combustion, and hydrologic export. Models predict that some portion of this release will be offset by increased production of Arctic and boreal biomass; however, the lack of robust estimates of net carbon balance increases the risk of further overshooting international emissions targets. Precise empirical or model-based assessments of the critical factors driving carbon balance are unlikely in the near future, so to address this gap, we present estimates from 98 permafrost-region experts of the response of biomass, wildfire, and hydrologic carbon flux to climate change. Results suggest that contrary to model projections, total permafrost-region biomass could decrease due to water stress and disturbance, factors that are not adequately incorporated in current models. Assessments indicate that end-of-the-century organic carbon release from Arctic rivers and collapsing coastlines could increase by 75% while carbon loss via burning could increase four-fold. Experts identified water balance, shifts in vegetation community, and permafrost degradation as the key sources of uncertainty in predicting future system response. In combination with previous findings, results suggest the permafrost region will become a carbon source to the atmosphere by 2100 regardless of warming scenario but that 65%-85% of permafrost carbon release can still be avoided if human emissions are actively reduced. C1 [Abbott, Benjamin W.] Univ Rennes 1, OSUR, CNRS, UMR ECOBIO 6553, F-35014 Rennes, France. [Abbott, Benjamin W.; Jones, Jeremy B.; Chapin, F. Stuart, III; Bret-Harte, M. Syndonia; Harms, Tamara K.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK USA. [Abbott, Benjamin W.; Jones, Jeremy B.; Chapin, F. Stuart, III; Bret-Harte, M. Syndonia; Harms, Tamara K.] Univ Alaska Fairbanks, Dept Biology& Wildlife, Fairbanks, AK USA. [Schuur, Edward A. G.; Mack, Michelle C.; Schaedel, Christina] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Bowden, William B.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Epstein, Howard E.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22903 USA. [Flannigan, Michael D.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2M7, Canada. [Hollingsworth, Teresa N.] Univ Alaska Fairbanks, PNW Res Stn, USDA Forest Serv, Fairbanks, AK USA. [McGuire, A. David] Univ Alaska Fairbanks, Alaska Cooperat Fish & Wildlife Res Unit, US Geol Survey, Anchorage, AK USA. [Natali, Susan M.; Goetz, Scott J.; Rogers, Brendan M.] Woods Hole Res Ctr, Woods Hole, MA USA. [Rocha, Adrian V.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Rocha, Adrian V.] Univ Notre Dame, Environm Change Initiat, Notre Dame, IN 46556 USA. [Tank, Suzanne E.] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2M7, Canada. [Turetsky, Merritt R.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Vonk, Jorien E.] Vrije Univ Amsterdam, Dept Earth Sci, Amsterdam, Netherlands. [Wickland, Kimberly P.; Aiken, George R.; Striegl, Robert G.] US Geol Survey, Natl Res Program, Boulder, CO USA. [Alexander, Heather D.] Mississippi State Univ, Forest & Wildlife Res Ctr, Mississippi State, MS 39762 USA. [Amon, Rainer M. W.] Texas A&M Univ, Galveston, TX USA. [Benscoter, Brian W.] Florida Atlantic Univ, Boca Raton, FL 33431 USA. [Bergeron, Yves] Univ Quebec Abitibi Temiscamingue, Forest Res Inst, Rouyn Noranda, PQ, Canada. [Bishop, Kevin] Uppsala Univ, Dept Earth Sci, Uppsala, Sweden. [Bishop, Kevin] Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, S-90183 Umea, Sweden. [Blarquez, Olivier] Univ Montreal, Dept Geog, Montreal, PQ H3C 3J7, Canada. [Bond-Lamberty, Ben] Pacific NW Natl Lab, Richland, WA 99352 USA. [Breen, Amy L.] Univ Alaska Fairbanks, Int Arctic Res Ctr, Scenarios Network Alaska & Arctic Planning, Fairbanks, AK USA. [Buffam, Ishi] Univ Cincinnati, Cincinnati, OH 45221 USA. [Cai, Yihua] Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen, Peoples R China. [Carcaillet, Christopher] Ecole Prat Hautes Etud, UMR5023, CNRS Lyon 1, Lyon, France. [Carey, Sean K.] McMaster Univ, Hamilton, ON L8S 4L8, Canada. [Chen, Jing M.] Univ Toronto, Toronto, ON M5S 1A1, Canada. [Chen, Han Y. H.] Lakehead Univ, Fac Nat Resources Management, Thunder Bay, ON P7B 5E1, Canada. [Christensen, Torben R.] Lund Univ, Arctic Res Ctr, S-22100 Lund, Sweden. [Christensen, Torben R.] Aarhus Univ, DK-8000 Aarhus C, Denmark. [Cooper, Lee W.] Univ Maryland, Ctr Environm Sci, Bethesda, MD USA. [Cornelissen, J. Hans C.] Vrije Univ Amsterdam, Syst Ecol, Amsterdam, Netherlands. [de Groot, William J.] Nat Resources Canada, Canadian Forest Serv, Toronto, ON, Canada. [DeLuca, Thomas H.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Dorrepaal, Ellen; Karlsson, Jan; Keuper, Frida] Umea Univ, Dept Ecol & Environm Sci, Climate Impacts Res Ctr, S-90187 Umea, Sweden. [Fetcher, Ned] Wilkes Univ, Inst Environm Sci & Sustainabil, Wilkes Barre, PA 18766 USA. [Finlay, Jacques C.] Univ Minnesota, Dept Ecol Evolut & Behav, Minneapolis, MN 55455 USA. [Forbes, Bruce C.] Univ Lapland, Arctic Ctr, Rovaniemi, Finland. [French, Nancy H. F.] Michigan Technol Univ, Michigan Tech Res Inst, Houghton, MI 49931 USA. [Gauthier, Sylvie; Girardin, Martin P.; Pare, David] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Toronto, ON, Canada. [Goldammer, Johann G.] Max Planck Inst Chem, Global Fire Monitoring Ctr, Berlin, Germany. [Gough, Laura] Towson Univ, Dept Biol Sci, Towson, MD USA. [Grogan, Paul] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada. [Guo, Laodong] Univ Wisconsin Milwaukee, Sch Freshwater Sci, Milwaukee, WI USA. [Higuera, Philip E.] Univ Montana, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. [Hinzman, Larry] Univ Alaska Fairbanks, Fairbanks, AK USA. [Hu, Feng Sheng] Univ Illinois, Dept Plant Biol, Chicago, IL 60680 USA. [Hu, Feng Sheng] Univ Illinois, Dept Geol, Chicago, IL 60680 USA. [Hugelius, Gustaf] Stockholm Univ, Dept Phys Geog, Stockholm, Sweden. [Jafarov, Elchin E.] Univ Colorado Boulder, Inst Arctic & Alpine Res, Boulder, CO USA. [Jandt, Randi] Univ Alaska Fairbanks, Alaska Fire Sci Consortium, Fairbanks, AK USA. [Johnstone, Jill F.] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 0W0, Canada. [Kasischke, Eric S.] Univ Maryland, Dept Geog Sci, Bethesda, MD USA. [Kattner, Gerhard] Helmholtz Ctr Polar & Marine Res, Alfred Wegener Inst, Berlin, Germany. [Kelly, Ryan] Neptune & Co Inc, North Wales, PA USA. [Keuper, Frida] INRA, AgroImpact UPR1158, New York, NY USA. [Kling, George W.] Univ Michigan, Ann Arbor, MI 48109 USA. [Kortelainen, Pirkko] Finnish Environm Inst, Helsinki, Finland. [Kouki, Jari] Univ Eastern Finland, Sch Forest Sci, Joensuu, Finland. [Kuhry, Peter] Stockholm Univ, Dept Phys Geog, Stockholm, Sweden. [Laudon, Hjalmar] Swedish Univ Agr Sci, Dept Forest Ecol & Management, S-90183 Umea, Sweden. [Laurion, Isabelle] Inst Natl Rech Sci, Ctr Eau Terre Environm, Toronto, ON, Canada. [Macdonald, Robie W.] Inst Ocean Sci, Dept Fisheries & Oceans, Toronto, ON, Canada. [Mann, Paul J.] Northumbria Univ, Dept Geog, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, England. [Martikainen, Pertti J.] Univ Eastern Finland, Dept Environm & Biol Sci, Joensuu, Finland. [McClelland, James W.] Univ Texas Austin, Inst Marine Sci, Austin, TX 78712 USA. [Molau, Ulf] Univ Gothenburg, Dept Biol & Environm Sci, Gothenburg, Sweden. [Oberbauer, Steven F.] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA. [Olefeldt, David] Univ Alberta, Dept Revewable Resources, Edmonton, AB T6G 2M7, Canada. [Parisien, Marc-Andre] Nat Resources Canada, Canadian Forest Serv, No Forestry Ctr, Toronto, ON, Canada. [Payette, Serge] Univ Laval, Ctr Etud Nord, Quebec City, PQ G1K 7P4, Canada. [Peng, Changhui] Univ Quebec, Ctr CEF, ESCER, Montreal, PQ H3C 3P8, Canada. [Peng, Changhui] Northwest A&F Univ, Coll Forestry, State Key Lab Soil Eros & Dryland Farming Loess P, Xian, Peoples R China. [Pokrovsky, Oleg S.] CNRS, Georesources & Environm, Toulouse, France. [Pokrovsky, Oleg S.] Tomsk State Univ, BIO GEO CLIM Lab, Tomsk, Russia. [Rastetter, Edward B.] Marine Biol Lab, Ctr Ecosyst, Woods Hole, MA 02543 USA. [Raymond, Peter A.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06520 USA. [Raynolds, Martha K.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK USA. [Rein, Guillermo] Univ London Imperial Coll Sci Technol & Med, Dept Mech Engn, London SW7 2AZ, England. [Reynolds, James F.] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China. [Reynolds, James F.] Duke Univ, Nicholas Sch Environm, Durham, NC 27706 USA. [Robards, Martin] Arctic Beringia Program, Wildlife Conservat Soc, New York, NY USA. [Schaefer, Kevin] Univ Colorado Boulder, Cooperat Inst Res Environm Sci, Natl Snow & Ice Data Ctr, Boulder, CO USA. [Schmidt, Inger K.] Univ Copenhagen, Dept Geosci & Nat Resource Management, DK-1168 Copenhagen, Denmark. [Shvidenko, Anatoly] Int Inst Appl Syst Anal, A-2361 Laxenburg, Austria. [Shvidenko, Anatoly] Sukachev Inst Forest, Moscow, Russia. [Sky, Jasper] Cambridge Ctr Climate Change Res, Cambridge, England. [Spencer, Robert G. M.] Florida State Univ, Dept Earth Ocean & Atmospher Sci, Tallahassee, FL 32306 USA. [Starr, Gregory] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Teisserenc, Roman] Univ Toulouse, CNRS, INPT, ECOLAB,UPS, Toulouse, France. [Tranvik, Lars J.] Uppsala Univ, Dept Ecol & Genet, Limnol, Uppsala, Sweden. [Virtanen, Tarmo] Univ Helsinki, Dept Environm Sci, FIN-00014 Helsinki, Finland. [Welker, Jeffrey M.] Univ Alaska Anchorage, Anchorage, AK USA. [Zimov, Sergei] Russian Acad Sci, Northeast Sci Stn, Moscow 117901, Russia. RP Abbott, BW (reprint author), Univ Rennes 1, OSUR, CNRS, UMR ECOBIO 6553, F-35014 Rennes, France.; Abbott, BW (reprint author), Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK USA.; Abbott, BW (reprint author), Univ Alaska Fairbanks, Dept Biology& Wildlife, Fairbanks, AK USA. EM benabbo@gmail.com RI Chen, Han/A-1359-2008; Vonk, Jorien/H-5422-2011; Tank, Suzanne/I-4816-2012; Forbes, Bruce/L-4431-2013; McClelland, James/C-5396-2008; Macdonald, Robie/A-7896-2012; Higuera, Philip/B-1330-2010; Olefeldt, David/E-8835-2013; Carcaillet, Christopher/G-1218-2011; Cooper, Lee/E-5251-2012; Shvidenko, Anatoly/I-1505-2016; Finlay, Jacques/B-6081-2011; Benscoter, Brian/K-8105-2016; Bond-Lamberty, Ben/C-6058-2008; Raymond, Peter/C-4087-2009; OI Chen, Han/0000-0001-9477-5541; Buffam, Ishi/0000-0002-2625-6640; JAFAROV, ELCHIN/0000-0002-8310-3261; Rastetter, Edward/0000-0002-8620-5431; Rein, Guillermo/0000-0001-7207-2685; Wickland, Kimberly/0000-0002-6400-0590; Abbott, Benjamin/0000-0001-5861-3481; Tank, Suzanne/0000-0002-5371-6577; Forbes, Bruce/0000-0002-4593-5083; McClelland, James/0000-0001-9619-8194; Macdonald, Robie/0000-0002-1141-8520; Higuera, Philip/0000-0001-5396-9956; Olefeldt, David/0000-0002-5976-1475; Carcaillet, Christopher/0000-0002-6632-1507; Cooper, Lee/0000-0001-7734-8388; Shvidenko, Anatoly/0000-0001-7640-2151; Finlay, Jacques/0000-0002-7968-7030; Benscoter, Brian/0000-0002-2706-4667; Bond-Lamberty, Ben/0000-0001-9525-4633; Raymond, Peter/0000-0002-8564-7860; Johnstone, Jill/0000-0001-6131-9339 FU National Science Foundation ARCSS program and Vulnerability of Permafrost Carbon Research Coordination Network [OPP-0806465, OPP-0806394, 955713]; SITES (Swedish Science Foundation); Future Forest (Mistra); Marie Curie International Reintegration Grant within the 7th European Community Framework Programme. [277059] FX Peter J Fix provided valuable input on the methods and manuscript. This work was supported by the National Science Foundation ARCSS program and Vulnerability of Permafrost Carbon Research Coordination Network (grants OPP-0806465, OPP-0806394, and 955713) with additional funding from SITES (Swedish Science Foundation), Future Forest (Mistra), and a Marie Curie International Reintegration Grant (TOMCAR-Permafrost #277059) within the 7th European Community Framework Programme. This work complies with the US HHS Policy for Protection of Human Research Subjects. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 86 TC 8 Z9 8 U1 44 U2 98 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 034014 DI 10.1088/1748-9326/11/3/034014 PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400017 ER PT J AU Giordano, MR Chong, J Weise, DR Asa-Awuku, AA AF Giordano, Michael R. Chong, Joey Weise, David R. Asa-Awuku, Akua A. TI Does chronic nitrogen deposition during biomass growth affect atmospheric emissions from biomass burning? SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE biomass burning; anthropogenic climate effects; air quality; climate; emissions ID SAN-BERNARDINO MOUNTAINS; FIRE RADIATIVE POWER; SOUTHERN CALIFORNIA; PONDEROSA PINE; ENVIRONMENTAL CHAMBER; TROPOSPHERIC NO2; ORGANIC AEROSOL; PARTICLE MASS; MOBILITY; FOREST AB Chronic nitrogen deposition has measureable impacts on soil and plant health. We investigate burning emissions from biomass grown in areas of high and low NOx deposition. Gas and aerosol-phase emissions were measured as a function of photochemical aging in an environmental chamber at UC-Riverside. Though aerosol chemical speciation was not available, results indicate a systemic compositional difference between biomass grown in high and low deposition areas. Aerosol emissions from biomass grown in areas of high NOx deposition exhibit a lower volatility than biomass grown in a low deposition area. Furthermore, fuel elemental analysis, NOx emission rates, and aerosol particle number distributions differed significantly between the two sites. Despite the limited scale of fuels explored, there is strong evidence that the atmospheric emissions community must pay attention to the regional air quality of biomass fuels growth areas. C1 [Giordano, Michael R.; Asa-Awuku, Akua A.] Univ Calif Riverside, Bourns Coll Engn, Dept Chem & Environm Engn, Riverside, CA 92521 USA. [Giordano, Michael R.; Asa-Awuku, Akua A.] Bourns Coll Engn, Ctr Environm Res & Technol CE CERT, Riverside, CA 92507 USA. [Chong, Joey; Weise, David R.] US Forest Serv, USDA, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. [Giordano, Michael R.] Drexel Univ, Dept Civil Architectural & Environm Engn, Philadelphia, PA 19104 USA. RP Asa-Awuku, AA (reprint author), Univ Calif Riverside, Bourns Coll Engn, Dept Chem & Environm Engn, Riverside, CA 92521 USA.; Asa-Awuku, AA (reprint author), Bourns Coll Engn, Ctr Environm Res & Technol CE CERT, Riverside, CA 92507 USA. EM akua@engr.ucr.edu FU USDA Forest Service Joint Venture Agreement [13-JV-11272167-062] FX This work has been supported by a USDA Forest Service Joint Venture Agreement 13-JV-11272167-062. The authors would also like to thank David Lyons of the UCR Environmental Science Research Laboratory for conducting EA analysis of fuel sampling. The authors appreciate the assistance of Dr Mark Fenn and Antonio Davila related to the two San Bernardino Mountains Gradient study sample sites. NR 54 TC 0 Z9 0 U1 6 U2 11 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 034007 DI 10.1088/1748-9326/11/3/034007 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400010 ER PT J AU Groffman, PM Grove, JM Polsky, C Bettez, ND Morse, JL Cavender-Bares, J Hall, SJ Heffernan, JB Hobbie, SE Larson, KL Neill, C Nelson, K Ogden, L O'Neil-Dunne, J Pataki, D Chowdhury, RR Locke, DH AF Groffman, Peter M. Grove, J. Morgan Polsky, Colin Bettez, Neil D. Morse, Jennifer L. Cavender-Bares, Jeannine Hall, Sharon J. Heffernan, James B. Hobbie, Sarah E. Larson, Kelli L. Neill, Christopher Nelson, Kristen Ogden, Laura O'Neil-Dunne, Jarlath Pataki, Diane Chowdhury, Rinku Roy Locke, Dexter H. TI Satisfaction, water and fertilizer use in the American residential macrosystem SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE lawns; water; nitrogen; environmental satisfaction; residential land use; urban ecology ID UNITED-STATES; LAWN-CARE; URBAN; MANAGEMENT; HOMOGENIZATION; PREDICTORS; VEGETATION; TURFGRASS; PATTERNS; IMPACTS AB Residential yards across the US look remarkably similar despite marked variation in climate and soil, yet the drivers of this homogenization are unknown. Telephone surveys of fertilizer and irrigation use and satisfaction with the natural environment, and measurements of inherent water and nitrogen availability in six US cities (Boston, Baltimore, Miami, Minneapolis-St. Paul, Phoenix, Los Angeles) showed that the percentage of people using irrigation at least once in a year was relatively invariant with little difference between the wettest (Miami, 85%) and driest (Phoenix, 89%) cities. The percentage of people using fertilizer at least once in a year also ranged narrowly (52%-71%), while soil nitrogen supply varied by 10x. Residents expressed similar levels of satisfaction with the natural environment in their neighborhoods. The nature and extent of this satisfaction must be understood if environmental managers hope to effect change in the establishment and maintenance of residential ecosystems. C1 [Groffman, Peter M.; Bettez, Neil D.] Cary Inst Ecosyst Studies, 2801 Sharon Turnpike, Millbrook, NY 12545 USA. [Grove, J. Morgan] US Forest Serv, USDA, Baltimore Field Stn, Suite 350,5523 Res Pk Dr, Catonsville, MD 21228 USA. [Polsky, Colin] Florida Atlantic Univ, Ctr Environm Studies, 3200 Coll Ave,Bldg DW 312, Davie, FL 33314 USA. [Morse, Jennifer L.] Portland State Univ, Dept Environm Sci & Management, POB 751 ESM, Portland, OR 97207 USA. [Cavender-Bares, Jeannine; Hobbie, Sarah E.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Hall, Sharon J.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Heffernan, James B.] Duke Univ, Nicholas Sch Environm, Box 90328, Durham, NC 27708 USA. [Larson, Kelli L.] Arizona State Univ, Sch Geog Sci & Urban Planning, Tempe, AZ 85287 USA. [Larson, Kelli L.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Neill, Christopher] Marine Biol Lab, Ctr Ecosyst, 7 MBL St, Woods Hole, MA 02543 USA. [Nelson, Kristen] Dept Forest Resources, 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. [Nelson, Kristen] Dept Fisheries Wildlife & Conservat Biol, 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. [Ogden, Laura] Dartmouth Coll, Dept Anthropol, 406A Silsby Hall, Hanover, NH 03755 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Spatial Anal Lab, Rubenstein Sch Environm & Nat Resources, Aiken Ctr 205E, 81 Carrigan Dr, Burlington, VT 05405 USA. [Pataki, Diane] Univ Utah, Dept Biol, 257 S 1400 E, Salt Lake City, UT 84112 USA. [Chowdhury, Rinku Roy; Locke, Dexter H.] Clark Univ, Grad Sch Geog, 950 Main St, Worcester, MA 01610 USA. [Groffman, Peter M.] CUNY, Adv Sci Res Ctr, 85 St Nicholas Terrace, New York, NY 10031 USA. RP Groffman, PM (reprint author), Cary Inst Ecosyst Studies, 2801 Sharon Turnpike, Millbrook, NY 12545 USA.; Groffman, PM (reprint author), CUNY, Adv Sci Res Ctr, 85 St Nicholas Terrace, New York, NY 10031 USA. EM peter.groffman@asrc.cuny.edu RI Heffernan, James/D-1261-2010; OI Heffernan, James/0000-0001-7641-9949; Hobbie, Sarah/0000-0001-5159-031X; Morse, Jennifer/0000-0001-8872-4940 FU MacroSystems Biology Program, in the Emerging Frontiers Division of the Biological Sciences Directorate at NSF; 'Ecological Homogenization of Urban America' project [EF-1065548, 1065737, 1065740, 1065741, 1065772, 1065785, 1065831, 121238320]; NSF Long Term Ecological Research Program in Baltimore [DEB-0423476]; NSF Long Term Ecological Research Program in Phoenix [BCS-1026865, DEB-0423704, DEB-9714833]; NSF Long Term Ecological Research Program in Plum Island (Boston) [OCE-1058747, 1238212]; NSF Long Term Ecological Research Program in Cedar Creek (Minneapolis- St. Paul) [DEB-0620652]; NSF Long Term Ecological Research Program in Florida Coastal Everglades (Miami) [DBI-0620409] FX We would like to acknowledge the MacroSystems Biology Program, in the Emerging Frontiers Division of the Biological Sciences Directorate at NSF for support. The 'Ecological Homogenization of Urban America' project was supported by a series of collaborative grants from this program (EF-1065548, 1065737, 1065740, 1065741, 1065772, 1065785, 1065831, 121238320). The work arose from research funded by grants from the NSF Long Term Ecological Research Program supporting work in Baltimore (DEB-0423476), Phoenix (BCS-1026865, DEB-0423704 and DEB-9714833), Plum Island (Boston) (OCE-1058747 and 1238212), Cedar Creek (Minneapolis- St. Paul) (DEB-0620652) and Florida Coastal Everglades (Miami) (DBI-0620409). NR 36 TC 0 Z9 0 U1 6 U2 19 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 034004 DI 10.1088/1748-9326/11/3/034004 PG 7 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400007 ER PT J AU Parks, SA Miller, C Abatzoglou, JT Holsinger, LM Parisien, MA Dobrowski, SZ AF Parks, Sean A. Miller, Carol Abatzoglou, John T. Holsinger, Lisa M. Parisien, Marc-Andre Dobrowski, Solomon Z. TI How will climate change affect wildland fire severity in the western US? SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE wildland fire; fire severity; fire regime; climate change; disequilibrium ID QUANTIFYING BURN SEVERITY; UNITED-STATES; SIERRA-NEVADA; SPECIES DISTRIBUTIONS; VEGETATION DYNAMICS; NORTH-AMERICA; WILDFIRE; FORESTS; DISTURBANCE; FUTURE AB Fire regime characteristics in North America are expected to change over the next several decades as a result of anthropogenic climate change. Although some fire regime characteristics (e.g., area burned and fire season length) are relatively well-studied in the context of a changing climate, fire severity has received less attention. In this study, we used observed data from 1984 to 2012 for the western United States (US) to build a statistical model of fire severity as a function of climate. We then applied this model to several (n = 20) climate change projections representing mid-century (2040-2069) conditions under the RCP 8.5 scenario. Model predictions suggest widespread reduction in fire severity for large portions of the western US. However, our model implicitly incorporates climate-induced changes in vegetation type, fuel load, and fire frequency. As such, our predictions are best interpreted as a potential reduction in fire severity, a potential that may not be realized due human-induced disequilibrium between plant communities and climate. Consequently, to realize the reductions in fire severity predicted in this study, land managers in the western US could facilitate the transition of plant communities towards a state of equilibrium with the emerging climate through means such as active restoration treatments (e.g., mechanical thinning and prescribed fire) and passive restoration strategies like managed natural fire (under suitable weather conditions). Resisting changes in vegetation composition and fuel load via activities such as aggressive fire suppression will amplify disequilibrium conditions and will likely result in increased fire severity in future decades because fuel loads will increase as the climate warms and fire danger becomes more extreme. The results of our study provide insights to the pros and cons of resisting or facilitating change in vegetation composition and fuel load in the context of a changing climate. C1 [Parks, Sean A.; Miller, Carol; Holsinger, Lisa M.] US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, 790 East Beckwith Ave, Missoula, MT 59801 USA. [Abatzoglou, John T.] Univ Idaho, Dept Geog, 875 Perimeter Dr MS3021, Moscow, ID 83844 USA. [Parisien, Marc-Andre] Nat Resources Canada, No Forestry Ctr, Canadian Forest Serv, 5320 122nd St, Edmonton, AB T5H 3S5, Canada. [Dobrowski, Solomon Z.] Univ Montana, Coll Forestry & Conservat, Dept Forest Management, 32 Campus Dr, Missoula, MT 59812 USA. RP Parks, SA (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, 790 East Beckwith Ave, Missoula, MT 59801 USA. EM sean_parks@fs.fed.us OI Abatzoglou, John/0000-0001-7599-9750 FU Rocky Mountain Research Station FX We acknowledge National Fire Plan Funding from the Rocky Mountain Research Station. We thank two anonymous reviewers for thoughtful comments that significantly improved this manuscript. NR 90 TC 5 Z9 5 U1 14 U2 41 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 035002 DI 10.1088/1748-9326/11/3/035002 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400030 ER PT J AU Tchebakova, NM Parfenova, EI Korets, MA Conard, SG AF Tchebakova, N. M. Parfenova, E. I. Korets, M. A. Conard, S. G. TI Potential change in forest types and stand heights in central Siberia in a warming climate SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE forest structure and productivity; bioclimatic models; climate change; central Siberia; the 21st century ID PINE SITE INDEX; RUSSIAN FORESTS; CARBON BALANCE; GLOBAL CHANGE; PERMAFROST; TEMPERATURE; PARAMETERS; INVERSION; IMPACTS; BIOMASS AB Previous regional studies in Siberia have demonstrated climate warming and associated changes in distribution of vegetation and forest types, starting at the end of the 20th century. In this study we used two regional bioclimatic envelope models to simulate potential changes in forest types distribution and developed new regression models to simulate changes in stand height in tablelands and southern mountains of central Siberia under warming 21st century climate. Stand height models were based on forest inventory data (2850 plots). The forest type and stand height maps were superimposed to identify how heights would change in different forest types in future climates. Climate projections from the general circulation model Hadley HadCM3 for emission scenarios B1 and A2 for 2080s were paired with the regional bioclimatic models. Under the harsh A2 scenario, simulated changes included: a 80%-90% decrease in forest-tundra and tundra, a 30% decrease in forest area, a similar to 400% increase in forest-steppe, and a 2200% increase in steppe, forest-steppe and steppe would cover 55% of central Siberia. Under sufficiently moist conditions, the southern and middle taiga were simulated to benefit from 21st century climate warming. Habitats suitable for highly-productive forests (>= 30-40 m stand height) were simulated to increase at the expense of less productive forests (10-20m). In response to the more extreme A2 climate the area of these highly-productive forests would increase 10%-25%. Stand height increases of 10 m were simulated over 35%-50% of the current forest area in central Siberia. In the extremely warm A2 climate scenario, the tall trees (25-30m) would occur over8%-12% of area in all forest types except forest-tundra by the end of the century. In forest-steppe, trees of 30-40 m may cover some 15% of the area under sufficient moisture. C1 [Tchebakova, N. M.; Parfenova, E. I.; Korets, M. A.] Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forests, Academgorodok 50-28, Krasnoyarsk 660036, Russia. [Conard, S. G.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59807 USA. RP Tchebakova, NM (reprint author), Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forests, Academgorodok 50-28, Krasnoyarsk 660036, Russia. EM ncheby@ksc.krasn.ru RI Korets, Mikhail/P-9487-2015 FU National Aeronautics and Space Administration (NASA); Land Cover Land Use Change (LCLUC) Science Program; Ministry of Education and Science of the Russian Federation [14.B25.31.0026]; Russian Foundation for Basic Research [16-05-00496] FX The authors gratefully acknowledge financial support for this research from the National Aeronautics and Space Administration (NASA), the Land Cover Land Use Change (LCLUC) Science Program, partial support by Grant 14.B25.31.0026 of the Ministry of Education and Science of the Russian Federation and partial support by Grant 16-05-00496 of the Russian Foundation for Basic Research. The authors are greatly indebted to Robert Monserud for providing his comments on science and help in language. NR 83 TC 0 Z9 0 U1 10 U2 18 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 035016 DI 10.1088/1748-9326/11/3/035016 PG 16 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400044 ER PT J AU Tian, XH Sohngen, B Kim, JB Ohrel, S Cole, J AF Tian, Xiaohui Sohngen, Brent Kim, John B. Ohrel, Sara Cole, Jefferson TI Global climate change impacts on forests and markets SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE climate change; dynamic optimization; markets ID TIMBER MARKETS; SECTOR; ECOSYSTEMS; PRODUCTS; DEMAND; MODEL AB This paper develops an economic analysis of climate change impacts in the global forest sector. It illustrates how potential future climate change impacts can be integrated into a dynamic forestry economics model using data from a global dynamic vegetation model, the MC2 model. The results suggest that climate change will cause forest outputs (such as timber) to increase by approximately 30% over the century. Aboveground forest carbon storage also is projected to increase, by approximately 26 Pg C by 2115, as a result of climate change, potentially providing an offset to emissions from other sectors. The effects of climate mitigation policies in the energy sector are then examined. When climate mitigation in the energy sector reduces warming, we project a smaller increase in forest outputs over the timeframe of the analysis, and we project a reduction in the sink capacity of forests of around 12 Pg C by 2115. C1 [Tian, Xiaohui] Renmin Univ, 59 Zhongguancun Ave,Room 919 Mingde Main Bldg, Beijing 100872, Peoples R China. [Sohngen, Brent] Ohio State Univ, 2120 Fyffe Rd, Columbus, OH 43210 USA. [Kim, John B.] US Forest Serv, Pacific NW Res Stn, USDA, 3200 SW Jefferson Way, Corvallis, OR 97330 USA. [Ohrel, Sara; Cole, Jefferson] US EPA, Climate Change Div, 1200 Penn Ave,NW 6207-J, Washington, DC 20460 USA. RP Tian, XH (reprint author), Renmin Univ, 59 Zhongguancun Ave,Room 919 Mingde Main Bldg, Beijing 100872, Peoples R China.; Sohngen, B (reprint author), Ohio State Univ, 2120 Fyffe Rd, Columbus, OH 43210 USA. EM tianxiaohui@ruc.edu.cn; Sohngen.1@osu.edu FU US Forest Service Western Wildland Environmental Threat Assessment Center; US Environmental Protection Agency's Climate Change Division [DW-012-92388301]; US Environmental Protection Agency Climate Change Division FX The authors appreciate funding from the US Environmental Protection Agency Climate Change Division although the analysis and conclusion do not in any way represent the views of the US Environmental Protection Agency Climate Change Division. John B Kim is supported in part by the US Forest Service Western Wildland Environmental Threat Assessment Center, and by US Environmental Protection Agency's Climate Change Division, under interagency agreement DW-012-92388301. NR 21 TC 0 Z9 0 U1 3 U2 11 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 035011 DI 10.1088/1748-9326/11/3/035011 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400039 ER PT J AU Williamson, GJ Prior, LD Jolly, WM Cochrane, MA Murphy, BP Bowman, DMJS AF Williamson, Grant J. Prior, Lynda D. Jolly, W. Matt Cochrane, Mark A. Murphy, Brett P. Bowman, David M. J. S. TI Measurement of inter- and intra-annual variability of landscape fire activity at a continental scale: the Australian case SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE climate variability; El Nino-Southern oscillation (ENSO); fire management; fire season; Indian Ocean dipole (IOD); pyrogeography ID SOUTH-EASTERN AUSTRALIA; INTERANNUAL VARIABILITY; INDIAN-OCEAN; CLIMATE; REGIMES; FUTURE; DANGER; DENDROCHRONOLOGY; CLASSIFICATION; SEASONALITY AB Climate dynamics at diurnal, seasonal and inter-annual scales shape global fire activity, although difficulties of assembling reliable fire and meteorological data with sufficient spatio-temporal resolution have frustrated quantification of this variability. Using Australia as a case study, we combine data from 4760 meteorological stations with 12 years of satellite-derived active fire detections to determine day and night time fire activity, fire season start and end dates, and inter-annual variability, across 61 objectively defined climate regions in three climate zones (monsoon tropics, arid and temperate). We show that geographic patterns of landscape burning (onset and duration) are related to fire weather, resulting in a latitudinal gradient from the monsoon tropics in winter, through the arid zone in all seasons except winter, and then to the temperate zone in summer and autumn. Peak fire activity precedes maximum lightning activity by several months in all regions, signalling the importance of human ignitions in shaping fire seasons. We determined median daily McArthur forest fire danger index (FFDI50) for days and nights when fires were detected: FFDI50 varied substantially between climate zones, reflecting effects of fire management in the temperate zone, fuel limitation in the arid zone and abundance of flammable grasses in the monsoon tropical zone. We found correlations between the proportion of days when FFDI exceeds FFDI50 and the Southern Oscillation index across the arid zone during spring and summer, and Indian Ocean dipole mode index across south-eastern Australia during summer. Our study demonstrates that Australia has a long fire weather season with high inter-annual variability relative to all other continents, making it difficult to detect long term trends. It also provides a way of establishing robust baselines to track changes to fire seasons, and supports a previous conceptual model highlighting multi-temporal scale effects of climate in shaping continental-scale pyrogeography. C1 [Williamson, Grant J.; Prior, Lynda D.; Bowman, David M. J. S.] Univ Tasmania, Sch Biol Sci, Private Bag 55, Hobart, Tas 7001, Australia. [Jolly, W. Matt] US Forest Serv, USDA, RMRS, Fire Sci Lab, Missoula, MT 59808 USA. [Cochrane, Mark A.] S Dakota State Univ, Geospatial Sci Ctr Excellence, Brookings, SD 57007 USA. [Murphy, Brett P.] Charles Darwin Univ, Res Inst Environm & Livelihoods, Darwin, NT 0909, Australia. RP Williamson, GJ (reprint author), Univ Tasmania, Sch Biol Sci, Private Bag 55, Hobart, Tas 7001, Australia. EM grant.williamson@utas.edu.au RI Prior, Lynda/A-7460-2012; Bowman, David/A-2930-2011 OI Prior, Lynda/0000-0002-5511-2320; Bowman, David/0000-0001-8075-124X FU NASA Interdisciplinary Sciences Grant [374 (NNX11AB89G)] FX NASA Interdisciplinary Sciences Grant 374 (NNX11AB89G) funded this research. NR 69 TC 4 Z9 4 U1 12 U2 21 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAR PY 2016 VL 11 IS 3 AR 035003 DI 10.1088/1748-9326/11/3/035003 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DI3LY UT WOS:000373401400031 ER PT J AU Hansen, A Ireland, K Legg, K Keane, R Barge, E Jenkins, M Pillet, M AF Hansen, Andrew Ireland, Kathryn Legg, Kristin Keane, Robert Barge, Edward Jenkins, Martha Pillet, Michiel TI Complex Challenges of Maintaining Whitebark Pine in Greater Yellowstone under Climate Change: A Call for Innovative Research, Management, and Policy Approaches SO FORESTS LA English DT Review DE climate change; Greater Yellowstone Ecosystem; resource management; policy; whitebark pine ID NORTHERN ROCKY-MOUNTAINS; SUB-ALPINE FORESTS; BLISTER RUST; BARK BEETLES; CLARKS NUTCRACKER; VEGETATION MODEL; BRITISH-COLUMBIA; NATIONAL-PARKS; ECOSYSTEM; FUTURE AB Climate suitability is projected to decline for many subalpine species, raising questions about managing species under a deteriorating climate. Whitebark pine (WBP) (Pinus albicaulis) in the Greater Yellowstone Ecosystem (GYE) crystalizes the challenges that natural resource managers of many high mountain ecosystems will likely face in the coming decades. We review the system of interactions among climate, competitors, fire, bark beetles, white pine blister rust (Cronartium ribicola), and seed dispersers that make WBP especially vulnerable to climate change. A well-formulated interagency management strategy has been developed for WBP, but it has only been implemented across <1% of the species GYE range. The challenges of complex climate effects and land allocation constraints on WBP management raises questions regarding the efficacy of restoration efforts for WBP in GYE. We evaluate six ecological mechanisms by which WBP may remain viable under climate change: climate microrefugia, climate tolerances, release from competition, favorable fire regimes, seed production prior to beetle-induced mortality, and blister-rust resistant trees. These mechanisms suggest that WBP viability may be higher than previously expected under climate change. Additional research is warranted on these mechanisms, which may provide a basis for increased management effectiveness. This review is used as a basis for deriving recommendations for other subalpine species threatened by climate change. C1 [Hansen, Andrew; Ireland, Kathryn; Pillet, Michiel] Montana State Univ, Dept Ecol, POB 173460, Bozeman, MT 59717 USA. [Legg, Kristin] Natl Pk Serv, Inventory & Monitoring Div, Greater Yellowstone Network, 2327 Univ Way Ste 2, Bozeman, MT 59715 USA. [Keane, Robert] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 US Hwy 10, Missoula, MT 59808 USA. [Barge, Edward; Jenkins, Martha] Montana State Univ, Plant Sci & Plant Pathol Dept, POB 173150, Bozeman, MT 59717 USA. RP Hansen, A (reprint author), Montana State Univ, Dept Ecol, POB 173460, Bozeman, MT 59717 USA. EM hansen@montana.edu; kathryn.ireland@montana.edu; kristin_legg@nps.gov; rkeane@fs.fed.us; ebarge9@gmail.com; martha.l.jenkins@gmail.com; mdpillet@gmail.com OI Pillet, Michiel/0000-0003-3215-8585 FU North Central Climate Sciences Center; NASA Applied Sciences Program [10-BIOCLIM10-0034]; Montana NSF EPSCoR Initiative; Great Northern Conservation Cooperative [F15AC01086] FX Data were provided by the GYCC Whitebark Pine Subcommittee and by the NPS Greater Yellowstone Inventory and Monitoring Network. Early drafts of the manuscript were reviewed by Karl Buermeyer, Tony Chang, Jessie Logan, David Roberts, Monica Turner, and Cathy Whitlock. Linda Phillips conducted analyses of land allocation types within whitebark pines' current distribution. Tony Chang provided data and analysis of tree species distribution along the elevational gradient. Comments by four anonymous reviewers improved the manuscript. Funding was provided by the North Central Climate Sciences Center, the NASA Applied Sciences Program (10-BIOCLIM10-0034), the Montana NSF EPSCoR Initiative, and the Great Northern Conservation Cooperative (F15AC01086). Any use of trade, product, or firm names are descriptive and do not imply endorsement by the U.S. Government. NR 125 TC 1 Z9 1 U1 26 U2 42 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAR PY 2016 VL 7 IS 3 DI 10.3390/f7030054 PG 28 WC Forestry SC Forestry GA DI7RX UT WOS:000373700800007 ER PT J AU Sun, G Vose, JM AF Sun, Ge Vose, James M. TI Forest Management Challenges for Sustaining Water Resources in the Anthropocene SO FORESTS LA English DT Article DE disturbance; forest hydrology; drought; modeling; watershed management; urbanization; climate change ID LOBLOLLY-PINE PLANTATION; CLIMATE-CHANGE SCENARIOS; SOUTHERN UNITED-STATES; US NATIONAL FORESTS; STOMATAL CONDUCTANCE; THROUGHFALL REDUCTION; ECOSYSTEM FUNCTIONS; TEMPERATE FOREST; CHANGING CLIMATE; DROUGHT IMPACTS AB The Earth has entered the Anthropocene epoch that is dominated by humans who demand unprecedented quantities of goods and services from forests. The science of forest hydrology and watershed management generated during the past century provides a basic understanding of relationships among forests and water and offers management principles that maximize the benefits of forests for people while sustaining watershed ecosystems. However, the rapid pace of changes in climate, disturbance regimes, invasive species, human population growth, and land use expected in the 21st century is likely to create substantial challenges for watershed management that may require new approaches, models, and best management practices. These challenges are likely to be complex and large scale, involving a combination of direct and indirect biophysical watershed responses, as well as socioeconomic impacts and feedbacks. We discuss the complex relationships between forests and water in a rapidly changing environment, examine the trade-offs and conflicts between water and other resources, and propose new management approaches for sustaining water resources in the Anthropocene. C1 [Sun, Ge] US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. [Vose, James M.] US Forest Serv, USDA, Southern Res Stn, Ctr Integrated Forest Sci,Dept Forestry & Environ, Raleigh, NC 27695 USA. RP Sun, G (reprint author), US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. EM gesun@fs.fed.us; jvose@fs.fed.us NR 98 TC 0 Z9 0 U1 12 U2 26 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAR PY 2016 VL 7 IS 3 DI 10.3390/f7030068 PG 13 WC Forestry SC Forestry GA DI7RX UT WOS:000373700800025 ER PT J AU Thompson, MP Bowden, P Brough, A Scott, JH Gilbertson-Day, J Taylor, A Anderson, J Haas, JR AF Thompson, Matthew P. Bowden, Phil Brough, April Scott, Joe H. Gilbertson-Day, Julie Taylor, Alan Anderson, Jennifer Haas, Jessica R. TI Application of Wildfire Risk Assessment Results to Wildfire Response Planning in the Southern Sierra Nevada, California, USA SO FORESTS LA English DT Article DE incident management; fire management planning; effects analysis; risk-informed decision making ID WILDLAND FIRE; UNITED-STATES; NATIONAL FOREST; MANAGEMENT; EXPOSURE; TRANSMISSION; LANDSCAPE; SCALE AB How wildfires are managed is a key determinant of long-term socioecological resiliency and the ability to live with fire. Safe and effective response to fire requires effective pre-fire planning, which is the main focus of this paper. We review general principles of effective federal fire management planning in the U.S., and introduce a framework for incident response planning consistent with these principles. We contextualize this framework in relation to a wildland fire management continuum based on federal fire management policy in the U.S. The framework leverages recent advancements in spatial wildfire risk assessment-notably the joint concepts of in situ risk and source risk-and integrates assessment results with additional geospatial information to develop and map strategic response zones. We operationalize this framework in a geographic information system (GIS) environment based on landscape attributes relevant to fire operations, and define Potential wildland fire Operational Delineations (PODs) as the spatial unit of analysis for strategic response. Using results from a recent risk assessment performed on several National Forests in the Southern Sierra Nevada area of California, USA, we illustrate how POD-level summaries of risk metrics can reduce uncertainty surrounding potential losses and benefits given large fire occurrence, and lend themselves naturally to design of fire and fuel management strategies. To conclude we identify gaps, limitations, and uncertainties, and prioritize future work to support safe and effective incident response. C1 [Thompson, Matthew P.; Haas, Jessica R.] US Forest Serv, USDA, Missoula, MT 59801 USA. [Bowden, Phil; Brough, April; Anderson, Jennifer] US Forest Serv, USDA, Vallejo, CA 94592 USA. [Scott, Joe H.; Gilbertson-Day, Julie] Pyrologix LLC, Missoula, MT 59801 USA. [Taylor, Alan] US Forest Serv, USDA, Lee Vining, CA 93541 USA. RP Thompson, MP (reprint author), US Forest Serv, USDA, Missoula, MT 59801 USA. EM mpthompson02@fs.fed.us; pbowden@fs.fed.us; ambrough@fs.fed.us; joe.scott@pyrologix.com; jgilbertsonday@pyrologix.com; ataylor04@fs.fed.us; jenniferanderson@fs.fed.us; jrhaas@fs.fed.us FU National Fire Decision Support Center; Rocky Mountain Research Station FX The National Fire Decision Support Center and the Rocky Mountain Research Station supported this effort. Frankie Romero, David Chapman, and two anonymous reviewers provided useful comments and suggestions. NR 35 TC 1 Z9 1 U1 9 U2 13 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAR PY 2016 VL 7 IS 3 DI 10.3390/f7030064 PG 22 WC Forestry SC Forestry GA DI7RX UT WOS:000373700800016 ER PT J AU Serra, S Leisso, R Giordani, L Kalcsits, L Musacchi, S AF Serra, Sara Leisso, Rachel Giordani, Luca Kalcsits, Lee Musacchi, Stefano TI Crop Load Influences Fruit Quality, Nutritional Balance, and Return Bloom in 'Honeycrisp' Apple SO HORTSCIENCE LA English DT Article DE apple; crop load; I-AD; fruit quality parameters; fruit size; nutrition ID DRY-MATTER PRODUCTION; BITTER PIT; TREES; COLOR; DISORDERS; STORAGE; SIZE; PHOTOSYNTHESIS; SPECTROSCOPY; MATURITY AB The apple variety, 'Honeycrisp' has been extensively planted in North America during the last two decades. However, it suffers from several agronomic problems that limit productivity and postharvest quality. To reduce losses, new information is needed to better describe the impact of crop load on productivity and postharvest fruit quality in a desert environment and the major region where 'Honeycrisp' expansion is occurring. Here, 7-year-old 'Honeycrisp' trees on the M9-Nic29 rootstock (2.5 x 0.9 m) were hand thinned to five different crop loads [from 4.7 to 16.0 fruit/cm(2) of trunk cross-sectional area (TCSA)] to compare fruit quality, maturity, fruit size, elemental concentration, and return bloom. Fruit size distribution was affected by crop load. Trees with the highest crop load (16 fruit/cm(2)) produced smaller fruit. Index of absorbance difference (I-AD) measurements (absorption difference between 670 and 720 nm), a proxy indicator of the chlorophyll content below the skin of fruit measured by a DA-meter, were made shortly after harvest (T0) and after 6 months of storage (T1). Fruit from the trees with the lowest crop load had lower I-AD values indicating advanced fruit ripeness. The comparison between the I-AD classes at T0 and T1 showed that fruit belonging to the lowest I-AD class had significantly higher red blushed overcolor percentage, firmness, dry matter, and soluble solid content than those in the "most unripe" class (highest I-AD readings) regardless of crop load. The percentage of blushed color, firmness, titratable acidity (TA), soluble solids content, and dry matter were all higher in the lowest crop loads at both T0 and T1. Fruit calcium (Ca) concentration was lowest at the lowest crop load. The (K + Mg + N):Ca ratio decreased as crop load increased until a crop load of 11.3 fruit/cm(2), which was not significantly different from higher crop loads. For return bloom, the highest number of flower clusters per tree was reported for 4.7 fruit/cm(2) crop load, and generally it decreased as crop load increased. Here, we highlight the corresponding changes in fruit quality, storability, and elemental balance with tree crop load. To maintain high fruit quality and consistency in yield, careful crop load management is required to minimize bienniality and improve fruit quality and storability. C1 [Serra, Sara; Leisso, Rachel; Giordani, Luca; Kalcsits, Lee; Musacchi, Stefano] Washington State Univ, Ctr Tree Fruit Res & Extens, Wenatchee, WA 98801 USA. [Serra, Sara; Leisso, Rachel; Giordani, Luca; Kalcsits, Lee; Musacchi, Stefano] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Leisso, Rachel] ARS, Tree Fruit Res Lab, USDA, Wenatchee, WA 98801 USA. RP Musacchi, S (reprint author), Washington State Univ, Ctr Tree Fruit Res & Extens, Wenatchee, WA 98801 USA.; Musacchi, S (reprint author), Washington State Univ, Dept Hort, Pullman, WA 99164 USA. EM stefano.musacchi@wsu.edu NR 48 TC 2 Z9 2 U1 6 U2 19 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2016 VL 51 IS 3 BP 236 EP 244 PG 9 WC Horticulture SC Agriculture GA DI7DR UT WOS:000373659800005 ER PT J AU Sun, QR Sun, MJ Sun, HY Bell, RL Li, LG Zhang, W Tao, JH AF Sun, Qingrong Sun, Meijuan Sun, Hongyan Bell, Richard L. Li, Linguang Zhang, Wei Tao, Jihan TI Comparative Organogenic Response of Six Clonal Apple Rootstock Cultivars SO HORTSCIENCE LA English DT Article DE leaf explants; shoot regeneration; bud regeneration; rooting; apple clonal rootstocks ID ADVENTITIOUS SHOOT REGENERATION; IN-VITRO; LEAF EXPLANTS; PYRUS-COMMUNIS; COLD-HARDY; ROLB GENE; THIDIAZURON; LEAVES; PEAR; INDUCTION AB The organogenesis potential is different among cultivars and must be optimized for individual genotype. Shoot organogenesis capacity from in vitro leaves and root organogenesis capacity of in vitro shoots in six clonal apple rootstock cultivars were compared. The shoot organogenesis capacity was highly genotype dependent. 'GM256' was found to be the most responsive genotype for shoot regeneration from leaf explants among the cultivars, showing high regeneration percentage on all tested media. The effects of basal medium composition and cytokinins on shoot regeneration were different depending on rootstock genotype. Optimum regeneration occurred on Murashige and Skoog (MS) basal medium for '71-3-150', and optimum regeneration occurred on Quoirin and Lepoivre (QL) basal medium for '60-160' and : Thidiazuron (TDZ) was more effective than 6-benzylaminopurine (BA) for Malus prunifolia (Y), whereas TDZ and BA were not significantly different for the other cultivars. All rootstock cultivars showed high root organogenic capacity. The percentage of rooting reached more than 90% and the mean root number per plantlet ranged from three to five. The optimum rooting medium was different for different rootstock cultivars. Optimum root organogenesis occurred on half-strength QL medium for 'GM256' and 'Y', and for and 'JM7' on one-quarter-strength MS medium. C1 [Sun, Qingrong; Sun, Hongyan; Li, Linguang; Tao, Jihan] Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China. [Sun, Meijuan] Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China. [Bell, Richard L.] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV 25430 USA. [Zhang, Wei] Shandong Yaoxiang Forestry Ctr, Tai An 271043, Shandong, Peoples R China. RP Sun, QR (reprint author), Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China. EM qingrong_sun@yahoo.com FU Improved Variety Program of Shandong Province of China [(2014)96] FX This study was supported by Improved Variety Program of Shandong Province of China [(2014)96]. NR 55 TC 0 Z9 0 U1 6 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2016 VL 51 IS 3 BP 272 EP 278 PG 7 WC Horticulture SC Agriculture GA DI7DR UT WOS:000373659800010 ER PT J AU Ledbetter, CA AF Ledbetter, Craig A. TI 'Goshen Gold': A Late-season Apricot for Fresh and Dried Product Markets SO HORTSCIENCE LA English DT Article DE fruit breeding; fruit quality; Prunus armeniaca C1 [Ledbetter, Craig A.] USDA ARS, Crop Dis Pests & & Genet Res Unit, 9611 South Riverbend Ave, Parlier, CA 93648 USA. RP Ledbetter, CA (reprint author), USDA ARS, Crop Dis Pests & & Genet Res Unit, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM craigledbetter@ars.usda.gov NR 6 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAR PY 2016 VL 51 IS 3 BP 300 EP 301 PG 2 WC Horticulture SC Agriculture GA DI7DR UT WOS:000373659800015 ER PT J AU Pinto, JR Marshall, JD Dumroese, RK Davis, AS Cobos, DR AF Pinto, Jeremiah R. Marshall, John D. Dumroese, R. Kasten Davis, Anthony S. Cobos, Douglas R. TI Seedling establishment and physiological responses to temporal and spatial soil moisture changes SO NEW FORESTS LA English DT Article DE Ponderosa pine; Soil water potential; Container seedlings; Stocktype; Volumetric water content ID PONDEROSA PINE-SEEDLINGS; CARBON-ISOTOPE DISCRIMINATION; GAS-EXCHANGE; SURFACE-AREA; WATER-STRESS; ROOT-GROWTH; PSEUDOTSUGA-MENZIESII; CONIFEROUS FORESTS; PACIFIC-NORTHWEST; DROUGHT AB In many forests of the world, the summer season (temporal element) brings drought conditions causing low soil moisture in the upper soil profile (spatial element)-a potentially large barrier to seedling establishment. We evaluated the relationship between initial seedling root depth, temporal and spatial changes in soil moisture during drought after outplanting, and subsequent seedling performance using seedlings of Pinus ponderosa Laws. var. ponderosa grown in three containers similar in dimension except for depth (i. e. three stocktypes). Soil moisture patterns were quantified and growth, gas exchange, and carbon isotope analysis were used as metrics for stocktype evaluation. Soil moisture reached minimum volumetric soil moisture contents (theta) of 0.078 m(3) m(-3) at a 15 cm depth and 0.15 m(3) m(-3) at 90 cm by late summer, which also translated to estimated soil water potential (Psi(soil)) values of -2.29 and -0.02 MPa, respectively. Seedling photosynthesis (A) and transpiration (E) rates followed soil moisture trends, also reaching seasonal lows in late summer. In early fall, gas exchange rates nearly doubled following a replenishment of upper-profile soil moisture by precipitation. Over the course of the growing season, stocktypes did not differ in gas exchange rates (P >= 0.15), biomass (P >= 0.45), root penetration depth (P = 0.60), or carbon isotope signature (P >= 0.60). For all seedlings, current-year needles showed greater capacity for A than previous-year needles (P <= 0.01), and A was only significantly correlated with soil moisture in the upper soil profile (15 cm; P <= 0.03). In this study, stocktype was not a significant factor, suggesting that seedling access to soil moisture was not different among them. The temporal and spatial variation observed in soil moisture availability, however, provides critical biophysical information on outplanting timing as it relates to subsequent seedling establishment and potential root growth. As well, needle formation, carbon gain, and the relationship to soil water depth further indicate the importance for managing soil water or seedling stocktype for successful seedling survival and growth. C1 [Pinto, Jeremiah R.; Dumroese, R. Kasten] USDA, Forest Serv, Rocky Mt Res Stn, 1221 S Main St, Moscow, ID 83843 USA. [Marshall, John D.; Davis, Anthony S.] Univ Idaho, Dept Forest Range & Fire Sci, Moscow, ID 83843 USA. [Cobos, Douglas R.] Decagon Devices, 2365 NE Hopkins Ct, Pullman, WA 99163 USA. RP Pinto, JR (reprint author), USDA, Forest Serv, Rocky Mt Res Stn, 1221 S Main St, Moscow, ID 83843 USA. EM jpinto@fs.fed.us FU USDA Forest Service FX We thank the Confederated Tribes of the Colville Indian Reservation for donating seeds and a planting unit for the project; Dr. Deborah Page-Dumroese for use of the soils lab and equipment; Ann Abbott and Scott Baggett for providing statistical guidance; and the editor and the anonymous reviewers for their constructive comments. Funding support was provided by the USDA Forest Service. NR 63 TC 0 Z9 0 U1 11 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 EI 1573-5095 J9 NEW FOREST JI New For. PD MAR PY 2016 VL 47 IS 2 BP 223 EP 241 DI 10.1007/s11056-015-9511-7 PG 19 WC Forestry SC Forestry GA DI7MU UT WOS:000373685700004 ER PT J AU Clark, SL Schlarbaum, SE Saxton, AM Hebard, FV AF Clark, Stacy L. Schlarbaum, Scott E. Saxton, Arnold M. Hebard, Frederick V. TI Establishment of American chestnuts (Castanea dentata) bred for blight (Cryphonectria parasitica) resistance: influence of breeding and nursery grading SO NEW FORESTS LA English DT Article DE Artificial regeneration; Cryphonectria parasitica; Exotic plant disease; Genetic family; Restoration; Visual grading ID NORTHERN RED OAK; QUERCUS-RUBRA; PLANTING STOCK; UNITED-STATES; SOUTHERN APPALACHIANS; BIOLOGICAL-CONTROL; FOREST ECOSYSTEM; NATIONAL FOREST; GROWTH-RATE; SEEDLINGS AB European and American chestnut species (Castanea) have been decimated by exotic species, most notably chestnut blight (Cryphonectria parasitica), since the early nineteenth century. Backcross breeding programs that transfer blight disease resistance from Chinese chestnut (C. mollissima) into American chestnut (C. dentata) offer promise for chestnut restoration, particularly for the American chestnut which was a keystone species in eastern North America. Nursery prescriptions and conformity to desired American chestnut traits following planting must be tested, however, before blight resistance can even be evaluated. We tested early field performance of American and Chinese chestnut and hybrid seedlings from the third backcross generation (e. g., BC3F3) in two-aged regeneration harvests on highly productive sites in the southern Appalachians, USA. We also tested a common nursery prescription of grading seedlings by size prior to planting. BC3F3 seedlings had similar 4-year survival to American chestnut seedlings, but generally had smaller stem heights and ground-line diameters (GLD). Although blight had not yet substantially challenged some sites, the BC3F3 seedlings had blight incidence similar to the Chinese chestnut which was lower than the American chestnut. Visual seedling grading affected planting shock and stem height and GLD by the end of year 4. Large size-class seedlings had more stem dieback and 5 % lower survival compared to small size-class seedlings, but larger trees exhibited the same height in year 3 as small trees in year 4. Advanced breeding material (BC3F3) was successfully established during the stand initiation phase of forest development on highly productive sites, but deviations in desired growth rate of the American chestnut was evident. Visual grading of seedlings affected establishment of breeding material, and should be considered in the restoration process. C1 [Clark, Stacy L.] USDA, Southern Res Stn, Forest Serv, 2431 Joe Johnson Dr,Rm 274 Ellington Plant Sci, Knoxville, TN 37996 USA. [Schlarbaum, Scott E.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, 2431 Joe Johnson Dr,Rm 274 Ellington Plant Sci, Knoxville, TN 37996 USA. [Saxton, Arnold M.] Univ Tennessee, Dept Anim Sci, 208C Brehm Anim Sci Bldg, Knoxville, TN 37996 USA. [Hebard, Frederick V.] Amer Chestnut Fdn, 29010 Hawthorne Dr, Meadowview, VA 24361 USA. RP Clark, SL (reprint author), USDA, Southern Res Stn, Forest Serv, 2431 Joe Johnson Dr,Rm 274 Ellington Plant Sci, Knoxville, TN 37996 USA. EM stacyclark@fs.fed.us FU American Chestnut Foundation [10-CO-11330134-055, 11-CO-11330134-136, 14-CO-11330134-004]; U.S. Department of Agriculture, Forest Service; University of Tennessee, Tennessee Agricultural Experiment Station FX This work was partially supported by The American Chestnut Foundation Grants Program [collection Agreement Nos. 10-CO-11330134-055, 11-CO-11330134-136, 14-CO-11330134-004]; the U.S. Department of Agriculture, Forest Service; and the University of Tennessee, Tennessee Agricultural Experiment Station. The authors greatly appreciate field assistance provided by University of Tennessee (UT), Department of Forestry, Wildlife, and Fisheries research technician John Johnson. Assistance from other UT students and technicians is also greatly appreciated: Lucas Allen, David Griffin, Luke Haden, Brian Hughett, Jay Messer, Tracy Powers, Ami Sharp, and Alison Shimer. The authors would like to acknowledge Forest Service employees who provided assistance to implement and maintain this research project: John Blanton (retired), David Casey, Barbara Crane, Robert Lewis, Russ MacFarlane, Robert Makowski, Mark Miller, Jason Rodrigue, Jim Stelick, the late Don Tomzcak, and Tyler Williamson. Assistance with insect identification was provided by Albert Mayfield, Southern Research Station, USDA Forest Service and Ashley Case, University of North Carolina Asheville. Gary Griffin, American Chestnut Cooperators Foundation and emeritus Professor at Virginia Tech University, and Mike Saunders, Purdue University, as well as two anonymous reviewers provided thoughtful comments to improve this manuscript. NR 115 TC 0 Z9 0 U1 15 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 EI 1573-5095 J9 NEW FOREST JI New For. PD MAR PY 2016 VL 47 IS 2 BP 243 EP 270 DI 10.1007/s11056-015-9512-6 PG 28 WC Forestry SC Forestry GA DI7MU UT WOS:000373685700005 ER PT J AU Alonso, DG de Oliveira, RS Koskinen, WC Hall, K Constantin, J Mislankar, S AF Alonso, Diego Goncalves de Oliveira, Rubem Silverio, Jr. Koskinen, William Charles Hall, Kathleen Constantin, Jamil Mislankar, Suresh TI Sorption and desorption of indaziflam degradates in several agricultural soils SO SCIENTIA AGRICOLA LA English DT Article DE adsorption; hysteresis; metabolites; soil properties ID METABOLITES; ATRAZINE AB Processes regulating pesticide fate in the environment are influenced by the physicochemical properties of pesticides and soils. Sorption and desorption are important processes as they regulate the movement of pesticides in soil. Although sorption-desorption is widely studied for herbicides, studies involving their metabolites in soil are scarce. Sorption and desorption of indaziflam metabolites (indaziflam-triazinediamine (FDAT), indaziflam-triazine-indanone (ITI) and indaziflam-carboxilic acid (ICA)) were investigated in six Brazilian (BRA) soils and three United States (USA) soils with different physicochemical properties. The Freundlich equation described sorption of the metabolites for all soils (R-2 > 0.98; 1/n similar to 1). Sorption order (K-f) was ITI > ICA > FDAT. Mean values of K-f, oc were 453, 289, and 81 (BRA) and 444, 48, and 48 (USA) for metabolites ITI, ICA, and FDAT respectively. Desorption was hysteretic for all metabolites in all soils. These results suggest that these metabolites fall in the classification range of mobile to moderately mobile in soils. C1 [Alonso, Diego Goncalves; de Oliveira, Rubem Silverio, Jr.; Constantin, Jamil] Univ Estadual Maringa, Ctr Adv Studies Weed Res NAPD, Dept Agron, Av Colombo 579, BR-87020900 Maringa, Parana, Brazil. [Koskinen, William Charles] ARS, USDA, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Hall, Kathleen] Univ Minnesota, Dept Soil Water & Climate, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Mislankar, Suresh] Bayer Crop Sci LP, Environm Fate & Exposure Assessment, RTP, 2 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. RP de Oliveira, RS (reprint author), Univ Estadual Maringa, Ctr Adv Studies Weed Res NAPD, Dept Agron, Av Colombo 579, BR-87020900 Maringa, Parana, Brazil. EM rsojunior@uem.br FU CNPq; CAPES (Brazil) FX We thank CNPq and CAPES (Brazil) for financial support for this research. We also thank Bayer for supplying the analytical and radioactive materials; Luis Avila (UFPel, RS), Hugo Dan (FESURV, GO), and Miriam Inoue (UNEMAT, MT) for their help in collecting soil samples. NR 12 TC 0 Z9 0 U1 6 U2 12 PU UNIV SAO PAOLO PI CERQUERA CESAR PA AV DR ENEAS DE CARVALHO AGUIAR, 419, CERQUERA CESAR, SP 05403-000, BRAZIL SN 0103-9016 J9 SCI AGR JI Sci. Agric. PD MAR-APR PY 2016 VL 73 IS 2 BP 169 EP 176 DI 10.1590/0103-9016-2015-0106 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA DI4VE UT WOS:000373496700010 ER PT J AU Bacon, CW White, JF AF Bacon, Charles W. White, James F., Jr. TI Functions, mechanisms and regulation of endophytic and epiphytic microbial communities of plants SO SYMBIOSIS LA English DT Article DE Endophyte community; Plant stress tolerance; Pathogenicity suppression ID QUORUM-SENSING INHIBITORS; BIOLOGICAL NITROGEN-FIXATION; INDUCED SYSTEMIC RESISTANCE; INFECTED TALL FESCUE; FUNGAL ENDOPHYTES; CANDIDA-ALBICANS; BACILLUS-AMYLOLIQUEFACIENS; CELL COMMUNICATION; OSMOTIC ADJUSTMENT; STRESS TOLERANCE AB Over the past several decades, we have come to appreciate that healthy plants host, within and on the surfaces of their tissues, endophytic and epiphytic fungi and bacteria that do not cause disease. Individual species (typically endophytes) of plants have been found to fall largely into one or more of three major functional groups: 1) Microbes that alleviate abiotic stress of the host; 2) Microbes that defend hosts from biotic stress (pathogens and herbivores); and 3) Microbes that support the host nutritionally through increased nitrogen, phosphorus, iron, etc. This functional aspect of plant microbiomes raises the potential to design and construct microbiomes for crop plants in order to enhance their cultivation with reduced agrochemical inputs and at lower cost. In order to design and construct functional microbiomes, we must first develop an understanding of the mechanisms by which plant microbiomes function. Examples of hypotheses for the abiotic stress tolerance mechanism include: 1) Oxidative stress protection by increased production of antioxidants produced either by the microbes or by hosts in response to microbes; 2) Ethylene reduction by production of ACC deaminase; and 3) Ammonia or ammonium detoxification and consequent oxidative stress avoidance. Mechanisms to explain biotic stress resistance generally include production of anti-herbivore or anti-pathogen defensive compounds by the microbe or by the host in response to the microbe (i.e., induced systemic resistance). Examples of hypothesized mechanisms to explain microbe-mediated enhanced plant growth include: 1) Stimulation of plant growth due to growth regulator production by microbes; 2) Increased absorption of nutrients by plants from the rhizosphere due to activities of microbes on roots; and 3) Increased supply of nitrogen obtained directly from diazotrophic microbes in plants. Factors by which plant endophyte communities are regulated are hypothesized to involve host-produced compounds that modify behavior of endophytic microbes, often reducing growth rates and suppressing pathogenic behaviors. These behavior-modifying compounds are proposed to include phenolic acids, quorum quenching compounds, and perhaps other secondary metabolites. C1 [Bacon, Charles W.] ARS, USDA, US Natl Poultry Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. [White, James F., Jr.] Rutgers State Univ, Dept Plant Biol, 59 Dudley Rd, New Brunswick, NJ 08901 USA. RP White, JF (reprint author), Rutgers State Univ, Dept Plant Biol, 59 Dudley Rd, New Brunswick, NJ 08901 USA. EM charles.bacon@ars.usda.gov; white@rci.rutgers.edu NR 121 TC 0 Z9 0 U1 13 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0334-5114 EI 1878-7665 J9 SYMBIOSIS JI Symbiosis PD MAR PY 2016 VL 68 IS 1-3 BP 87 EP 98 DI 10.1007/s13199-015-0350-2 PG 12 WC Microbiology SC Microbiology GA DI8HZ UT WOS:000373743400010 ER PT J AU Gotardo, AT Pfister, JA Raspantini, PCF Gorniak, SL AF Gotardo, Andre T. Pfister, James A. Raspantini, Paulo C. F. Gorniak, Silvana L. TI Maternal Ingestion of Ipomoea carnea: Effects on Goat-Kid Bonding and Behavior SO TOXINS LA English DT Article DE reproductive toxicology; swainsonine; I. carnea; morning glory; neuroteratology; goats ID LOCOWEED ASTRAGALUS-LENTIGINOSUS; ALKALOID SWAINSONINE; LYSOSOMAL STORAGE; POISONOUS PLANT; SPATIAL MEMORY; SHEEP; RUMINANTS; TOXICITY; BIRTH; LAMB AB Ipomoea carnea is a toxic plant found in Brazil and other tropical and subtropical countries and often causes poisoning of livestock. The plant contains the alkaloids swainsonine and calystegines, which inhibit key cellular enzymes and cause systematic cell death. This study evaluated the behavioral effects of prenatal ingestion of this plant on dams and their kids. Twenty-four pregnant goats were randomly allocated into four treatment groups and received the following doses (g/kg BW) of fresh I. carnea: 0 (control group), 1.0 (IC1), 3.0 (IC3), and 5.0 (IC5) from day 27 of gestation until parturition. Dam and kid bonding and behavior were evaluated by several tests, immediately after birth until six weeks of age. Dams from IC3 and IC5 groups spent less time paying attention to the newborn. There was a lack of maternal-infant bonding due to I. carnea intoxication. Kids from treated dams had difficulty in standing, suckling, and in recognizing their mother hours after birth. I. carnea can also compromise the kids' ability to learn and to retain spatial memory. We suggest that kids from pregnant goats given I. carnea during gestation have significant behavioral alterations and developmental delays that may compromise their survival. C1 [Gotardo, Andre T.; Raspantini, Paulo C. F.; Gorniak, Silvana L.] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Res Ctr Vet Toxicol CEPTOX, BR-13635900 Pirassununga, Brazil. [Pfister, James A.] USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. RP Gorniak, SL (reprint author), Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Res Ctr Vet Toxicol CEPTOX, BR-13635900 Pirassununga, Brazil. EM andregotardo@gmail.com; jamesapfister@gmail.com; pcraspantini@gmail.com; gorniak@usp.br FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2006/58729-2] FX We thank Leonila Ester Reiner Raspantini, Estevao Belloni, Marco Antonio Faustino dos Santos, and Adilson Baladore for their valuable assistance with the study and for the animal care. This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Proc No. 2006/58729-2). NR 80 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD MAR PY 2016 VL 8 IS 3 AR 74 DI 10.3390/toxins8030074 PG 17 WC Toxicology SC Toxicology GA DI6HM UT WOS:000373599800014 ER PT J AU Pilarska, D Hajek, AE Keena, M Linde, A Kereselidze, M Georgiev, G Georgieva, M Mirchev, P Takov, D Draganova, S AF Pilarska, Daniela Hajek, Ann E. Keena, Melody Linde, Andreas Kereselidze, Manana Georgiev, Georgi Georgieva, Margarita Mirchev, Plamen Takov, Danail Draganova, Slavimira TI Susceptibility of Larvae of Nun Moth, Lymantria monacha (Linnaeus, 1758) (Lepidoptera), to the Entomopathogenic Fungus Entomophaga maimaiga Humber, Shimazu and Soper (Entomophthorales) under Laboratory and Field Conditions SO ACTA ZOOLOGICA BULGARICA LA English DT Article DE host specificity; physiological host range; ecological host range; Lymantria monacha; Lymantria dispar; Entomophaga maimaiga ID DISPAR L. LEPIDOPTERA; GYPSY-MOTH; 1ST RECORD; RESTING SPORES; ENTOMOPHTORALES ENTOMOPHTORACEAE; NORTH-AMERICAN; HOST-RANGE; PATHOGEN; POPULATIONS; BULGARIA AB Susceptibility of Lymantria monacha larvae to Entomophaga maimaiga was investigated under laboratory and field conditions, using larvae of the natural host, Lymantria dispar, as positive controls. In laboratory bioassays, L. monacha and L. dispar were injected with protoplasts of two isolates of E. maimaiga and mortality was monitored for 20 days. While virtually all injected L. dispar died, with ST50s (median survival times for 50% of insects injected with the two isolates) of six seven days, only 65.6-86.7% of the injected L. monacha died, with ST50s of 11-17 days. Both isolates produced conidia and resting spores more frequently within dead L. dispar than L. monacha. In more ecologically relevant host range assays, larvae of both species were exposed to germinating soil-borne E. maimaiga resting spores in the laboratory. More L. dispar than L. monacha larvae died after these exposures. However, while resting spores were formed within 100% of L. dispar larvae that died, significantly fewer (10%) dead L. monacha contained resting spores. When L. monacha larvae were collected during an E. maimaiga epizootic occurring in a sympatric L. dispar population, only 0.2 % of the L. monacha died and produced spores. These findings corroborate those of previous studies reporting a narrow host range for this fungal pathogen. C1 [Pilarska, Daniela; Takov, Danail] Bulgarian Acad Sci, Inst Biodivers & Ecosyst Res, 1 Tsar Osvoboditel Blvd, Sofia 1000, Bulgaria. [Hajek, Ann E.] Cornell Univ, Dept Entomol, 6126 Comstock Hall, Ithaca, NY 14853 USA. [Keena, Melody] US Forest Serv, USDA, No Res Stn, 51 Mill Pond Rd, Hamden, CT 06514 USA. [Linde, Andreas] Eberswalde Univ Sustainable Dev, Alfred Moller Str, D-16225 Eberswalde, Germany. [Kereselidze, Manana] Agr Univ Georgia, V Gulisashvili Forest Inst, 240 D Aghmashenebeli Alley, GE-0159 Tbilisi, Rep of Georgia. [Georgiev, Georgi; Georgieva, Margarita; Mirchev, Plamen] Bulgarian Acad Sci, Forest Res Inst, 132 St Kliment Ohridski Blvd, Sofia 1756, Bulgaria. [Draganova, Slavimira] Inst Soil Sci Agrotechnol & Plant Protect, 7 Shosse Bankya St, Sofia 1080, Bulgaria. [Pilarska, Daniela] Czech Univ Life Sci, Fac Forestry & Wood Sci, 1176 Str Kamycka, CZ-16521 Prague, Czech Republic. [Kereselidze, Manana] Sci Res Ctr Agr, 8 St Chachava, GE-0159 Tbilisi, Rep of Georgia. RP Pilarska, D (reprint author), Bulgarian Acad Sci, Inst Biodivers & Ecosyst Res, 1 Tsar Osvoboditel Blvd, Sofia 1000, Bulgaria.; Hajek, AE (reprint author), Cornell Univ, Dept Entomol, 6126 Comstock Hall, Ithaca, NY 14853 USA.; Pilarska, D (reprint author), Czech Univ Life Sci, Fac Forestry & Wood Sci, 1176 Str Kamycka, CZ-16521 Prague, Czech Republic. FU German Academic Exchange Service (DAAD) FX We thank Alice Vandel for help with bioassays, Nancy Moran for use of her sterile hood and Tarryn Goble for assistance with survival analyses. The authors are grateful to the German Academic Exchange Service (DAAD) and to the staff of the Landeskompetenzzentrum Forest Eberswalde (LFE) for supporting this study. We are very grateful to eng. Maria Matova (FRI), the specialists from the Forest Protection Station, Plovdiv, Bulgaria (eng. Hristo Tomovski, eng. Peter Terziev, eng. Rumen Nachev, eng. Maria Dobreva) and State Forestry, Kirkovo (eng. Yulian Kehaiov, eng. Ilia Minchev) for their help in introduction of E. maimaiga in L. dispar populations in the Kirkovo region and collection of biological material. NR 47 TC 0 Z9 0 U1 3 U2 4 PU INST ZOOLOGY, BAS PI SOFIA PA 1000 SOFIA, 1, TSAR OSVOBODITEL BLVD, SOFIA, 00000, BULGARIA SN 0324-0770 J9 ACTA ZOOL BULGAR JI Acta Zool. Bulg. PD MAR PY 2016 VL 68 IS 1 BP 117 EP 126 PG 10 WC Zoology SC Zoology GA DI1HC UT WOS:000373246200017 ER PT J AU Hahn, W Stewart, H Blayney, DP Davis, CG AF Hahn, William Stewart, Hayden Blayney, Donald P. Davis, Christopher G. TI Modeling price transmission between farm and retail prices: a soft switches approach SO AGRICULTURAL ECONOMICS LA English DT Article DE Smooth transition autoregressive; Price transmission; Asymmetry; Cheddar cheese; Mozzarella cheese ID ERROR-CORRECTION; COINTEGRATION; THRESHOLD; REPRESENTATION; MARKETS AB Vector error correction models (VECM) are used to model price transmission when farm and retail prices are cointegrated. To allow for nonlinearity in the cointegration process, researchers may specify thresholds to break the error correction process into regimes according to whether the retail price is above, below, or close to its equilibrium value given farm prices. However, because the coefficients in a VECM can change when there is movement from one regime to another, the model can be discontinuous. This implies sudden, hard regime changes. In this study, we extend the threshold VECM to include features of smooth transition autoregression (STAR) models. Our approach allows for gradual, soft regime changes.An empirical application to retail cheese and farm milk prices is presented. C1 [Hahn, William; Stewart, Hayden; Davis, Christopher G.] USDA, Econ Res Serv, Washington, DC USA. [Blayney, Donald P.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Hahn, W (reprint author), USDA, Econ Res Serv, Washington, DC USA. EM whahn@ers.usda.gov NR 21 TC 0 Z9 0 U1 9 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0169-5150 EI 1574-0862 J9 AGR ECON-BLACKWELL JI Agric. Econ. PD MAR PY 2016 VL 47 IS 2 BP 193 EP 203 DI 10.1111/agec.12222 PG 11 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DH3AL UT WOS:000372659100005 ER PT J AU Motil, KJ Fete, M Fete, TJ AF Motil, Kathleen J. Fete, Mary Fete, Timothy J. TI Growth, nutritional, and gastrointestinal aspects of focal dermal hypoplasia (Goltz-Gorlin syndrome) SO AMERICAN JOURNAL OF MEDICAL GENETICS PART C-SEMINARS IN MEDICAL GENETICS LA English DT Article DE growth; body height; body weight; anthropometry; body fat; ectodermal dysplasia; short stature; malnutrition; gastroesophageal reflux; gastroparesis; constipation; gastrostomy ID ECTODERMAL DYSPLASIA SYNDROMES; REGULATOR; PORCN; FAT AB Focal dermal hypoplasia (FDH) is a rare genetic disorder caused by mutations in the PORCN gene located on the X-chromosome. In the present study, we characterized the pattern of growth, body composition, and the nutritional and gastrointestinal aspects of children and adults (n=19) affected with this disorder using clinical anthropometry and a survey questionnaire. The mean birth length (P<0.06) and weight (P<0.001) z-scores of the participants were lower than the reference population. The mean head circumference (P<0.001), height (length) (P<0.001), weight (P<0.01), and BMI (P<0.05) for age z-scores of the participants were lower than the reference population. The height-for-age and weight-for-age z-scores of the participants did not differ significantly between birth and current measurements. Three-fourths of the group reported having one or more nutritional or gastrointestinal problems including short stature (65%), underweight (77%), oral motor dysfunction (41%), gastroesophageal reflux (24%), gastroparesis (35%), and constipation (35%). These observations provide novel clinical information about growth, body composition, and nutritional and gastrointestinal aspects of children and adults with FDH and underscore the importance of careful observation and early clinical intervention in the care of individuals affected with this disorder. (c) 2016 Wiley Periodicals, Inc. C1 [Motil, Kathleen J.] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Washington, DC 20250 USA. [Motil, Kathleen J.] Texas Childrens Hosp, Sect Pediat Gastroenterol Hepatol & Nutr, Houston, TX 77030 USA. [Fete, Timothy J.] Univ Missouri Columbia, Sch Med, Dept Child Hlth, Columbia, MO USA. [Fete, Mary; Fete, Timothy J.] Natl Fdn Ectodermal Dysplasias, Fairview Heights, IL USA. RP Motil, KJ (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM kmotil@bcm.edu FU National Foundation for Ectodermal Dysplasias; Agricultural Research Service of the United States Department of Agriculture [58-7MN1-6-100] FX This work is a publication of the United States Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX. This project has been funded in part from the National Foundation for Ectodermal Dysplasias and with federal funds from the Agricultural Research Service of the United States Department of Agriculture under Cooperative Agreement number 58-7MN1-6-100. The contents of this publication do not necessarily reflect the views or policies of the National Foundation of Ectodermal Dysplasias or the United States Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the United States government. The authors thank the families who participated in the research workshop. NR 16 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1552-4868 EI 1552-4876 J9 AM J MED GENET C JI Am. J. Med. Genet. C PD MAR PY 2016 VL 172 IS 1 SI SI BP 29 EP 33 DI 10.1002/ajmg.c.31468 PG 5 WC Genetics & Heredity SC Genetics & Heredity GA DH7VQ UT WOS:000373002400007 PM 27001925 ER PT J AU Quecine, MC Kidarsa, TA Goebel, NC Shaffer, BT Henkels, MD Zabriskie, TM Loper, JE AF Quecine, Maria Carolina Kidarsa, Teresa A. Goebel, Neal C. Shaffer, Brenda T. Henkels, Marcella D. Zabriskie, T. Mark Loper, Joyce E. TI An Interspecies Signaling System Mediated by Fusaric Acid Has Parallel Effects on Antifungal Metabolite Production by Pseudomonas protegens Strain Pf-5 and Antibiosis of Fusarium spp. SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BIOSYNTHETIC GENE-CLUSTER; FLUORESCENS PF-5; BIOLOGICAL-CONTROL; SOIL SUPPRESSIVENESS; CHLORORAPHIS PCL1391; COTTON SEEDLINGS; RHIZOXIN ANALOGS; DAMPING-OFF; ROOT-ROT; PCR DATA AB Pseudomonas protegens strain Pf-5 is a rhizosphere bacterium that suppresses soilborne plant diseases and produces at least seven different secondary metabolites with antifungal properties. We derived mutants of Pf-5 with single and multiple mutations in biosynthesis genes for seven antifungal metabolites: 2,4-diacetylphoroglucinol ( DAPG), pyrrolnitrin, pyoluteorin, hydrogen cyanide, rhizoxin, orfamide A, and toxoflavin. These mutants were tested for inhibition of the pathogens Fusarium verticillioides and Fusarium oxysporum f. sp. pisi.Rhizoxin, pyrrolnitrin, and DAPG were found to be primarily responsible for fungal antagonism by Pf-5. Previously, other workers showed that the mycotoxin fusaric acid, which is produced by many Fusarium species, including F. verticillioides, inhibited the production of DAPG by Pseudomonas spp. In this study, amendment of culture media with fusaric acid decreased DAPG production, increased pyoluteorin production, and had no consistent influence on pyrrolnitrin or orfamide A production by Pf-5. Fusaric acid also altered the transcription of biosynthetic genes, indicating that the mycotoxin influenced antibiotic production by Pf-5 at the transcriptional level. Addition of fusaric acid to the culture medium reduced antibiosis of F. verticillioides by Pf-5 and derivative strains that produce DAPG but had no effect on antibiosis by Pf-5 derivatives that suppressed F. verticillioides due to pyrrolnitrin or rhizoxin production. Our results demonstrated the importance of three compounds, rhizoxin, pyrrolnitrin, and DAPG, in suppression of Fusarium spp. by Pf-5 and confirmed that an interspecies signaling system mediated by fusaric acid had parallel effects on antifungal metabolite production and antibiosis by the bacterial biological control organism. C1 [Quecine, Maria Carolina] Univ Sao Paulo, Dept Genet, Coll Agr Luiz de Queiroz, ESALQ, Sao Paulo, Brazil. [Kidarsa, Teresa A.; Shaffer, Brenda T.; Henkels, Marcella D.; Loper, Joyce E.] ARS, USDA, Corvallis, OR USA. [Goebel, Neal C.; Zabriskie, T. Mark] Oregon State Univ, Dept Pharmaceut Sci, Corvallis, OR 97331 USA. [Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Goebel, Neal C.] US Navy Drug Screening Lab, Jacksonville, FL USA. RP Loper, JE (reprint author), ARS, USDA, Corvallis, OR USA.; Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM loperj@science.oregonstate.edu RI Quecine, Maria/N-4478-2016; OI Quecine, Maria/0000-0002-9781-8479; Verdi, Maria/0000-0002-9524-941X; Loper, Joyce/0000-0003-3501-5969 FU USDA| National Institute of Food and Agriculture (NIFA) [2006-35319-17427, 2011-67019-30192]; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2005-53748-6] FX USDA| National Institute of Food and Agriculture (NIFA) provided funding to Joyce E. Loper under grant number 2006-35319-17427. USDA | National Institute of Food and Agriculture (NIFA) provided funding to T. Mark Zabriskie and Joyce E. Loper under grant number 2011-67019-30192. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) provided funding to Maria Carolina Quecine under grant number 2005-53748-6. NR 68 TC 2 Z9 2 U1 14 U2 32 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2016 VL 82 IS 5 BP 1372 EP 1382 DI 10.1128/AEM.02574-15 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2PL UT WOS:000373338800002 ER PT J AU Diodati, ME Bates, AH Miller, WG Carter, MQ Zhou, Y Brandl, MT AF Diodati, M. E. Bates, A. H. Miller, W. G. Carter, M. Q. Zhou, Y. Brandl, M. T. TI The Polymorphic Aggregative Phenotype of Shiga Toxin-Producing Escherichia coli O111 Depends on RpoS and Curli SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID SPINACH-ASSOCIATED OUTBREAK; HEMOLYTIC-UREMIC SYNDROME; BIOFILM FORMATION; CSGD PROMOTER; SALMONELLA-TYPHIMURIUM; UNITED-STATES; EXPRESSION; STRAINS; SURFACES; O157/H7 AB Escherichia coli O111 is an emerging non-O157:H7 serotype of Shiga toxin-producing E. coli 9STEC). We previously reported that outbreak and environmental, but not sporadic-case, strains of STEC O111 share a distinct aggregation phenotype 9M.E. Diodati, A. H. Bates, M. B. Cooley, S. Walker, R. E. Mandrell, and M. T. Brandl, Foodborne Pathog Dis 12: 235 - 243, 2015, http://dx.doi.org/10.1089/fpd.2014.1887). We show here the natural occurrence of nonaggregative variants in single STEC O111 strains. These variants do not produce curli fimbriae and lack RpoS function but synthesize cellulose. The deletion of csgBAC or rpoS in an aggregative outbreak strain abolished aggregate formation, which was rescued when curli biogenesis or RpoS function, respectively, was restored. Complementation of a nonaggregative variant with RpoS also conferred curli production and aggregation. These observations were supported by Western blotting with an anti-CsgA antibody. Immunomicroscopy revealed that curli were undetectable on the cells of the nonaggregative variant and the RpoS mutant but were present in large quantities in the intercellular matrix of the assemblages formed by aggregative strains. Sequence analysis of rpoS in the aggregative strain and its variant showed a single substitution of threonine for asparagine at amino acid 124. Our results indicate that the multicellular behavior of STEC O111 is RpoS dependent via positive regulation of curli production. Aggregation may confer a fitness advantage in O111 outbreak strains under stressful conditions in hydrodynamic environments along the food production chain and in the host, while the occurrence of nonaggregative variants may allow the cell population to adapt to conditions benefiting a planktonic lifestyle. C1 [Diodati, M. E.; Bates, A. H.; Miller, W. G.; Carter, M. Q.; Zhou, Y.; Brandl, M. T.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. RP Brandl, MT (reprint author), ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. EM maria.brandl@ars.usda.gov FU USDA Agricultural Research Service (ARS) under CRIS project [2030-42000-046-00D] FX The USDA Agricultural Research Service (ARS) provided funding to Michelle E. Diodati, Anne H. Bates, William G. Miller, Michelle Q. Carter, Yaguang Zhou, and Maria T. Brandl under CRIS project 2030-42000-046-00D. NR 58 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2016 VL 82 IS 5 BP 1475 EP 1485 DI 10.1128/AEM.03935-15 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2PL UT WOS:000373338800012 ER PT J AU Chow, V Kim, YS Rhee, MS Sawhney, N St John, FJ Nong, G Rice, JD Preston, JF AF Chow, Virginia Kim, Young Sik Rhee, Mun Su Sawhney, Neha St John, Franz J. Nong, Guang Rice, John D. Preston, James F. TI A 1,3-1,4-beta-Glucan Utilization Regulon in Paenibacillus sp Strain JDR-2 SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ALDOURONATE UTILIZATION; DEPOLYMERIZATION; MECHANISM; CLONING AB Paenibacillus sp. strain JDR-2 (Paenibacillus JDR-2) secretes a multimodular cell-associated glycoside hydrolase family 10 (GH10) endoxylanase (XynA10A(1)) that catalyzes the depolymerization of methylglucuronoxylan (MeGX(n)) and rapidly assimilates the products of depolymerization. Efficient utilization of MeGXn has been postulated to result from the coupling of the processes of exocellular depolymerization and assimilation of oligosaccharide products, followed by intracellular metabolism. Growth and substrate utilization patterns with barley glucan and laminarin similar to those observed with MeGXn as a substrate suggest similar processes for 1,3-1,4-beta-glucan and 1,3-beta-glucan depolymerization and product assimilation. The Paenibacillus JDR-2 genome includes a cluster of genes encoding a secreted multimodular GH16 beta-glucanase (Bgl16A(1)) containing surface layer homology (SLH) domains, a secreted GH16 beta-glucanase with only a catalytic domain (Bgl16A(2)), transporter proteins, and transcriptional regulators. Recombinant Bgl16A(1) and Bgl16A(2) catalyze the formation of trisaccharides, tetrasaccharides, and larger oligosaccharides from barley glucan and of mono-, di-, tri-, and tetrasaccharides and larger oligosaccharides from laminarin. The lack of accumulation of depolymerization products during growth and a marked preference for polymeric glucan over depolymerization products support a process coupling extracellular depolymerization, assimilation, and intracellular metabolism for beta-glucans similar to that ascribed to the GH10/GH67 xylan utilization system in Paenibacillus JDR-2. Coordinate expression of genes encoding GH16 beta-glucanases, transporters, and transcriptional regulators supports their role as a regulon for the utilization of soluble beta-glucans. As in the case of the xylan utilization regulons, this soluble beta-glucan regulon provides advantages in the growth rate and yields on polymeric substrates and may be exploited for the efficient conversion of plant-derived polysaccharides to targeted products. C1 [Chow, Virginia; Kim, Young Sik; Rhee, Mun Su; Sawhney, Neha; Nong, Guang; Rice, John D.; Preston, James F.] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. [St John, Franz J.] US Forest Serv, Inst Microbial & Biochem Technol, Forest Prod Lab, USDA, Madison, WI USA. [Rhee, Mun Su] Xycrobe Therapeut Inc, San Diego, CA USA. RP Preston, JF (reprint author), Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. EM jpreston@ufl.edu FU Florida Energy Systems Consortium, State University System of Florida [00077818]; USDA | National Institute of Food and Agriculture (NIFA) [2011-10006-30358]; USDA Hatch Multistate Project through University of Florida Institute for Food and Agricultural Sciences [005137] FX Florida Energy Systems Consortium, State University System of Florida provided funding to James F. Preston under grant number 00077818. USDA | National Institute of Food and Agriculture (NIFA) provided funding to James F. Preston under grant number 2011-10006-30358.; General support is provided by USDA Hatch Multistate Project 005137 through the University of Florida Institute for Food and Agricultural Sciences. NR 26 TC 3 Z9 3 U1 2 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAR PY 2016 VL 82 IS 6 BP 1789 EP 1798 DI 10.1128/AEM.03526-15 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2PR UT WOS:000373339400015 PM 26746717 ER PT J AU Pantin-Jackwood, MJ Kapczynski, DR DeJesus, E Costa-Hurtado, M Dauphin, G Tripodi, A Dunn, JR Swayne, DE AF Pantin-Jackwood, Mary J. Kapczynski, Darrell R. DeJesus, Eric Costa-Hurtado, Mar Dauphin, Gwenaelle Tripodi, Astrid Dunn, John R. Swayne, David E. TI Efficacy of a Recombinant Turkey Herpesvirus H5 Vaccine Against Challenge With H5N1 Clades 1.1.2 and 2.3.2.1 Highly Pathogenic Avian Influenza Viruses in Domestic Ducks (Anas platyrhynchos domesticus) SO AVIAN DISEASES LA English DT Article DE highly pathogenic avian influenza; turkey herpesvirus-H5 vaccine; ducks; vaccines; vaccination ID VECTOR VACCINE; HPAI; PROTECTION; THAILAND; STRAINS AB Domestic ducks are the second most abundant poultry species in many Asian countries and have played a critical role in the epizootiology of H5N1 highly pathogenic avian influenza (HPAI). In this study, the protective efficacy of a live recombinant vector vaccine based on a turkey herpesvirus (HVT) expressing the H5 gene from a clade 2.2 H5N1 HPAI strain (A/Swan/Hungary/4999/2006) (rHVT-H5/2.2), given at 3 days of age, was examined in Pekin ducks (Anas platyrhynchos domesticus). The vaccine was given alone or in combination with an inactivated H5N1 clade 2.3.2.1 reverse genetic (rgGD/2.3.2.1) vaccine given at 16 days of age, either as a single vaccination or in a prime-boost regime. At 30 days of age, ducks were challenged with one of two H5N1 HPAI viruses: A/duck/Vietnam/NCVD-2721/2013 (clade 1.1.2) or A/duck/Vietnam/NCVD-1584/2012 (clade 2.3.2.1.C). These viruses produced 100% mortality in less than 5 days in nonvaccinated control ducks. Ducks vaccinated with the rgGD/2.3.2.1 vaccine, with or without the rHVT-H5/2.2 vaccine, were 90%-100% protected against mortality after challenge with either of the two H5N1 HPAI viruses. The rHVT-H5/2.2 vaccine alone, however, conferred only 30% protection against mortality after challenge with either H5N1 HPAI virus; the surviving ducks from these groups shed higher amount of virus and for longer than the single-vaccinated rgGD/2.3.2.1 group. Despite low protection, ducks vaccinated with the rHVT-H5/2.2 vaccine and challenged with the clade 1.1.2 Vietnam virus had a longer mean death time than nonvaccinated controls (P = 0.02). A booster effect was found on reduction of virus shedding when using both vaccines, with lower oropharyngeal viral titers at 4 days after challenge with either HPAI virus (P < 0.05). Neither rHVT-H5/2.2 nor standard HVT vaccine could be detected in samples collected from multiple tissues at different time points, indicting minimal levels of viral replication. In conclusion, although a minor effect on survival was observed, this study demonstrates the suboptimal protection with the rHVT-H5/2.2 vaccine given alone in Pekin ducks against H5N1 HPAI viruses and only a minor additive effect on virus shedding reduction when used with an inactivated vaccine in a prime-boost regime. C1 [Pantin-Jackwood, Mary J.; Kapczynski, Darrell R.; DeJesus, Eric; Costa-Hurtado, Mar; Swayne, David E.] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Dauphin, Gwenaelle; Tripodi, Astrid] Food & Agr Org United Nations, Anim Hlth Serv, Rome, Italy. [Dunn, John R.] ARS, Avian Dis & Oncol Lab, USDA, 4279 E Mt Hope Rd, E Lansing, MI 48823 USA. RP Pantin-Jackwood, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM mary.pantin-jackwood@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU USDA-ARS Current Research Information System project [6612-32000-063-00D]; Food and Agriculture Organization of the United Nations for the U.S. Agency for International Development [OSRO/GLO/301/USA] FX We appreciate the technical assistance provided by Diane Smith and Aniko Zsak and the animal care provided by Bill Gagnon, Keith Crawford, Gerald Damron, and Roger Brock in conducting these studies. We gratefully acknowledge Dr. Hualan Chen from Harbin Veterinary Research Institute for providing the plasmids to reconstitute the rgGD/2.3.2.1 virus. We thank Dr. Yangbin Li for rescuing the virus during a sabbatical at SEPRL. We also thank Dr. Tung Nguyen from the National Centre for Veterinary Diagnosis, Department of Animal Health, Hanoi, Viet Nam, for providing the challenge viruses. This research was supported by the USDA-ARS Current Research Information System project 6612-32000-063-00D and with funds from the Food and Agriculture Organization of the United Nations for the U.S. Agency for International Development-funded project OSRO/GLO/301/USA, "Vaccination of day-old chicks at hatchery level as a new tool for H5N1 avian influenza prevention and control." NR 25 TC 0 Z9 1 U1 3 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2016 VL 60 IS 1 BP 22 EP 32 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA DI2AK UT WOS:000373297900004 PM 26953940 ER PT J AU Kufuor-Mensah, E Reed, WM Sleight, S Pestka, J Fadly, AM Dunn, JR AF Kufuor-Mensah, E. Reed, W. M. Sleight, S. Pestka, J. Fadly, A. M. Dunn, J. R. TI Effects of T-2 Toxin on Turkey Herpesvirus-Induced Vaccinal Immunity Against Marek's Disease SO AVIAN DISEASES LA English DT Article DE Marek's disease; T-2 toxin; vaccination; mycotoxin ID INFECTIOUS BURSAL DISEASE; SIMPLEX-VIRUS TYPE-2; BROILER-CHICKENS; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; NATURAL OCCURRENCE; AFLATOXIN B-1; ORAL-EXPOSURE; ANEMIA VIRUS; MICE AB T-2 toxin, a very potent immunotoxic Type A trichothecene, is a secondary metabolite produced primarily by Fusarium spp., which grows on cereal grains and can lead to contaminated livestock feed. Repeated exposure to T-2 toxin has been shown to cause immunosuppression and decrease the resistance of exposed animals to a variety of infectious diseases; however, the effects of T-2 toxin on Marek's disease (MD) vaccinal immunity have not been reported. Four trials were conducted to determine the effects of T-2 toxin on vaccinal immunity against MD. Day-old, white leghorn chicks of Avian Disease and Oncology Laboratory line 15I(5) 6 71 were treated daily for 7 days via crop gavage with T-2 toxin at a sublethal dose of 1.25 mg/kg body weight. Treated and untreated chicks were also vaccinated with turkey herpesvirus (HVT) at hatch and were challenged with the JM strain of MD virus (MDV) at 8 days of age. Chickens were tested for HVT viremia at 1 wk postvaccination immediately before challenge, and for HVT and MDV viremia at 3 wk postchallenge. Chickens were observed for the development of MD lesions and mortality within 8 wk of age. T-2 toxin significantly reduced body weight and titers of HVT viremia within 7 days after hatch. T-2 toxin shortened the incubation period for the development of MD lesions and mortality, but only in unvaccinated chickens. The percent MD protection in T-2-toxin-treated, HVT-vaccinated chickens ranged from 82% to 96% and was comparable to that in HVT-vaccinated untreated control chickens (89%-100%). The data suggest that exposure of chickens to sublethal doses of T-2 toxin for 7 consecutive days after hatch may influence the development of 1) HVT viremia; and 2) MD lesions and mortality, but only in unvaccinated chickens. C1 [Kufuor-Mensah, E.; Fadly, A. M.; Dunn, J. R.] USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. [Kufuor-Mensah, E.; Reed, W. M.; Sleight, S.] Michigan State Univ, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA. [Kufuor-Mensah, E.] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Reed, W. M.] Antech Diagnost, 17672 Cowan Ave, Irvine, CA 92614 USA. [Pestka, J.] Purdue Univ, Coll Vet Med, W Lafayette, IN 47907 USA. RP Dunn, JR (reprint author), USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM john.dunn@ars.usda.gov FU U.S. Department of Agriculture FX We thank Melanie Flesberg and Barbara Riegle for their expert technical assistance and Dr. Richard Witter for his advice during the course of this study. This study was supported by U.S. Department of Agriculture in-house funding. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 76 TC 1 Z9 1 U1 1 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2016 VL 60 IS 1 BP 56 EP 62 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA DI2AK UT WOS:000373297900008 PM 26953944 ER PT J AU Silva, MSE Rissi, DR Swayne, DE AF Sa e Silva, Mariana Rissi, Daniel R. Swayne, David E. TI Very Virulent Infectious Bursal Disease Virus Produces More-Severe Disease and Lesions in Specific-Pathogen-Free (SPF) Leghorns Than in SPF Broiler Chickens SO AVIAN DISEASES LA English DT Article DE infectious bursal disease virus; rA strain; very virulent infectious bursal disease virus; white leghorn; white Plymouth rock; broiler; layer; chicken; pathogenesis ID LINES AB Infectious bursal disease virus (IBDV) is an important pathogen of chickens causing negative economic impacts in poultry industries worldwide. IBDV has a variable range of virulence, with very virulent (vvIBDV) strains being responsible for the greatest losses from mortality and decreased performance. Previous vvIBDV studies using conventional broilers reported resistance to lethal effects and decreased performance as compared to specific-pathogen-free (SPF) layers, but the potential contribution of the conventional vs. SPF status to resistance has not been examined. In this study we compared differences in the acute pathologic effects of infection by the California rA strain of vvIBDV for SPF white leghorn egg-laying chickens and SPF white Plymouth Rock broiler chickens over a 7-day experimental period. Based on the clinical signs and mortality observed, as well as on the more-severe pathologic changes in lymphoid tissues and kidneys, white leghorns were shown to be more susceptible to the deleterious effects of vvIBDV infection than were white Plymouth Rocks. This study provides important information on the impact of chicken breed on susceptibility to vvIBDV and the absence of impact from conventional vs. SPF status on the outcome. C1 [Sa e Silva, Mariana; Swayne, David E.] ARS, Southeast Poultry Res Lab, US Natl Poultry Res Ctr, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Rissi, Daniel R.] Univ Georgia, Dept Pathol, Coll Vet Med, Athens, GA 30606 USA. [Rissi, Daniel R.] Univ Georgia, Athens Vet Diagnost Lab, Coll Vet Med, Athens, GA 30606 USA. RP Swayne, DE (reprint author), ARS, Southeast Poultry Res Lab, US Natl Poultry Res Ctr, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.swayne@ars.usda.gov NR 10 TC 2 Z9 2 U1 2 U2 5 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAR PY 2016 VL 60 IS 1 BP 63 EP 66 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA DI2AK UT WOS:000373297900009 ER PT J AU Karkman, A Johnson, TA Lyra, C Stedtfeld, RD Tamminen, M Tiedje, JM Virta, M AF Karkman, Antti Johnson, Timothy A. Lyra, Christina Stedtfeld, Robert D. Tamminen, Manu Tiedje, James M. Virta, Marko TI High-throughput quantification of antibiotic resistance genes from an urban wastewater treatment plant SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE sludge; sewage; qPCR ID ACTIVATED-SLUDGE; METAGENOMIC APPROACH; BACTERIA; ENVIRONMENT; EFFLUENT AB Antibiotic resistance among bacteria is a growing problem worldwide, and wastewater treatment plants have been considered as one of the major contributors to the dissemination of antibiotic resistance to the environment. There is a lack of comprehensive quantitative molecular data on extensive numbers of antibiotic resistance genes (ARGs) in different seasons with a sampling strategy that would cover both incoming and outgoing water together with the excess sludge that is removed from the process. In order to fill that gap we present a highly parallel quantitative analysis of ARGs and horizontal gene transfer potential over four seasons at an urban wastewater treatment plant using a high-throughput qPCR array. All analysed transposases and two-thirds of primer sets targeting ARGs were detected in the wastewater. The relative abundance of most of the genes was highest in influent and lower in effluent water and sludge. The resistance profiles of the samples cluster by sample location with a shift from raw influent through the final effluents and dried sludge to the sediments. Wastewater discharge enriched only a few genes, namely Tn25 type transposase gene and clinical class 1 integrons, in the sediment near the discharge pipe, but those enriched genes may indicate a potential for horizontal gene transfer. C1 [Karkman, Antti; Lyra, Christina; Tamminen, Manu; Virta, Marko] Univ Helsinki, Dept Food & Environm Sci, FIN-00014 Helsinki, Finland. [Johnson, Timothy A.; Stedtfeld, Robert D.; Tiedje, James M.] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA. [Johnson, Timothy A.] USDA, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Agr Res Serv, Ames, IA USA. [Tamminen, Manu] ETH Zurich Switzerland & Aquat Ecol, Dept Environm Syst Sci, Eawag, Switzerland. RP Karkman, A (reprint author), Univ Helsinki, POB 56 00014, FIN-00014 Helsinki, Finland. EM antti.karkman@helsinki.fi RI Virta, Marko/B-4292-2010; OI Virta, Marko/0000-0001-5981-7566; Karkman, Antti/0000-0003-0983-3319 FU Academy of Finland FX The authors want to acknowledge the staff from HSY at Helsinki UWTP for providing the samples. This work has been financed through the project funded by Academy of Finland, in the frame of the collaborative international consortium StARE of the Water Challenges for a Changing World Joint Programming Initiative (Water JPI) Pilot Call. All authors declare they have no actual or potential competing financial interest. NR 31 TC 5 Z9 5 U1 20 U2 51 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0168-6496 EI 1574-6941 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD MAR PY 2016 VL 92 IS 3 AR fiw014 DI 10.1093/femsec/fiw014 PG 7 WC Microbiology SC Microbiology GA DI1ZP UT WOS:000373295800008 ER PT J AU Stedtfeld, RD Williams, MR Fakher, U Johnson, TA Stedtfeld, TM Wang, F Khalife, WT Hughes, M Etchebarne, BE Tiedje, JM Hashsham, SA AF Stedtfeld, Robert D. Williams, Maggie R. Fakher, Umama Johnson, Timothy A. Stedtfeld, Tiffany M. Wang, Fang Khalife, Walid T. Hughes, Mary Etchebarne, Brett E. Tiedje, James M. Hashsham, Syed A. TI Antimicrobial resistance dashboard application for mapping environmental occurrence and resistant pathogens SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE AR dashboard database application; antibiotic resistance gene qPCR array; environmental resistome; mobile genetic elements; mapping genes ID ANTIBIOTIC-RESISTANCE; RECEIVING RIVER; GENES; BACTERIA; DATABASE; IMPACT; POINT; CHINA; SOILS AB An antibiotic resistance (AR) Dashboard application is being developed regarding the occurrence of antibiotic resistance genes (ARG) and bacteria (ARB) in environmental and clinical settings. The application gathers and geospatially maps AR studies, reported occurrence and antibiograms, which can be downloaded for offline analysis. With the integration of multiple data sets, the database can be used on a regional or global scale to identify hot spots for ARGs and ARB; track and link spread and transmission, quantify environmental or human factors influencing presence and persistence of ARG harboring organisms; differentiate natural ARGs from those distributed via human or animal activity; cluster and compare ARGs connections in different environments and hosts; and identify genes that can be used as proxies to routinely monitor anthropogenic pollution. To initially populate and develop the AR Dashboard, a qPCR ARG array was tested with 30 surface waters, primary influent from three waste water treatment facilities, ten clinical isolates from a regional hospital and data from previously published studies including river, park soil and swine farm samples. Interested users are invited to download a beta version (available on iOS or Android), submit AR information using the application, and provide feedback on current and prospective functionalities. C1 [Stedtfeld, Robert D.; Williams, Maggie R.; Fakher, Umama; Stedtfeld, Tiffany M.; Hashsham, Syed A.] Michigan State Univ, Dept Civil & Environm Engn, A126 Res Complex Engn, E Lansing, MI 48824 USA. [Johnson, Timothy A.; Wang, Fang; Tiedje, James M.; Hashsham, Syed A.] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA. [Wang, Fang] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China. [Khalife, Walid T.] Sparrow Hlth Syst, Sparrow Labs, Dept Microbiol, Lansing, MI 48912 USA. [Hughes, Mary; Etchebarne, Brett E.] Michigan State Univ, Coll Osteopath Med, Sect Emergency Med, Dept Osteopath Med Specialties, E Lansing, MI 48824 USA. [Tiedje, James M.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. [Johnson, Timothy A.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Hashsham, SA (reprint author), Michigan State Univ, Dept Civil & Environm Engn, A126 Res Complex Engn, E Lansing, MI 48824 USA. EM hashsham@egr.msu.edu FU Pharmaceuticals in the Environment Initiative; Center for the Health Impacts of Agriculture (CHIA); Michigan State University Clinical Translational Science Institute Seed Grant [224]; Environmental Protection Agency Great lakes Restoration Initiative [GL-00E01127-0]; Superfund Research Program from the National Institute for Environmental Health Sciences [2 P42 ES004911-22A1] FX The research was funded in part by the Pharmaceuticals in the Environment Initiative, the Center for the Health Impacts of Agriculture (CHIA), Michigan State University Clinical Translational Science Institute Seed Grant App ID 224, and the Environmental Protection Agency Great lakes Restoration Initiative ( GL-00E01127-0), the Superfund Research Program ( 2 P42 ES004911-22A1) from the National Institute for Environmental Health Sciences. NR 39 TC 2 Z9 2 U1 12 U2 32 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0168-6496 EI 1574-6941 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD MAR PY 2016 VL 92 IS 3 AR fiw020 DI 10.1093/femsec/fiw020 PG 9 WC Microbiology SC Microbiology GA DI1ZP UT WOS:000373295800014 ER PT J AU Bradley, PW Brawner, MD Raffel, TR Rohr, JR Olson, DH Blaustein, AR AF Bradley, P. W. Brawner, M. D. Raffel, T. R. Rohr, J. R. Olson, D. H. Blaustein, A. R. TI Effect of temperature on chytridiomycosis in larval amphibians SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Oregon State Univ, Corvallis, OR 97331 USA. Oakland Univ, Rochester, MI 48063 USA. Univ S Florida, Tampa, FL USA. US Forest Serv, USDA, Washington, DC USA. Pacific NW Res Stn, Washington, DC USA. EM paul.bradley@science.oregonstate.edu NR 0 TC 0 Z9 0 U1 2 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA 125-3 BP E23 EP E23 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457600092 ER PT J AU Dowle, EJ Bracewell, R Pfrender, M Mock, K Bentz, B Ragland, G AF Dowle, E. J. Bracewell, R. Pfrender, M. Mock, K. Bentz, B. Ragland, G. TI Reproductive isolation and environmental adaptation shape the phylogeography of Mountain Pine Beetle (Dendroctonus ponderosae) SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Kansas State Univ, Manhattan, KS 66506 USA. Univ Montana, Missoula, MT 59812 USA. Univ Notre Dame, Notre Dame, IN 46556 USA. Utah State Univ, Logan, UT 84322 USA. USDA, Rocky Mt Res Stn, Washington, DC USA. EM eddydowle@ksu.edu NR 0 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA 92-2 BP E57 EP E57 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457600226 ER PT J AU Hardwick, KM Perkin, LC Schlenke, TA Hardwick, K AF Hardwick, K. M. Perkin, L. C. Schlenke, T. A. Hardwick, Kayla TI Transcriptional response of Drosophila melanogaster to infection by ten different species of parasitic wasps SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Reed Coll, Portland, OR USA. USDA, Washington, DC USA. EM hardwickk@reed.edu NR 0 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA 105-2 BP E84 EP E84 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457600336 ER PT J AU Helm, BR Baldwin, MD Rinehart, JP Yocum, GD Bowsher, JH Greenlee, KJ AF Helm, B. r Baldwin, M. D. Rinehart, J. P. Yocum, G. D. Bowsher, J. H. Greenlee, K. J. TI Causes of metamorphic induction and consequences for adult phenotypes in solitary bees SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 N Dakota State Univ, Fargo, ND USA. ARS, USDA, Insect Genet & Biochem, Washington, DC USA. EM bryan.r.helm@ndsu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA 80-8 BP E88 EP E88 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457600350 ER PT J AU Midford, PE Clark, AB Margulis, SW Parr, CS AF Midford, P. E. Clark, A. B. Margulis, S. W. Parr, C. S. TI A Newly Integrated Ontology for Behavioral Biology: NBO meets ABO SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Binghamton Univ, Binghamton, NY USA. Canisius Coll, Buffalo, NY USA. USDA ARS, Washington, DC 20250 USA. EM peter.midford@gmail.com NR 0 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA P3.189 BP E334 EP E334 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601692 ER PT J AU Patel, S Zachry, J Kimball, BA Parker, MR AF Patel, S. Zachry, J. Kimball, B. A. Parker, M. R. TI Estrogen implantation alters putative pheromone composition in male brown tree snakes SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Washington & Lee Univ, Lexington, VA 24450 USA. USDA, Washington, DC USA. Monell Chem Senses Ctr, Philadelphia, PA USA. James Madison Univ, Harrisonburg, VA USA. EM mrockwellparker@gmail.com NR 0 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA P2.115 BP E347 EP E347 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601747 ER PT J AU Ritzenthaler, CA Litton, CM Giardina, CP Pelini, SL AF Ritzenthaler, C. A. Litton, C. M. Giardina, C. P. Pelini, S. L. TI Soil moisture and millipede abundance are more important drivers of macroinvertebrate diversity than temperature in Hawaiian forest SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Bowling Green State Univ, Bowling Green, OH 43403 USA. Univ Hawaii Manoa, Honolulu, HI 96822 USA. Inst Pacific Isl Forestry, Hilo, HI USA. US Forest Serv, USDA, Washington, DC USA. EM carir@bgsu.edu RI Giardina, Christian/C-3120-2011 OI Giardina, Christian/0000-0002-3431-5073 NR 0 TC 0 Z9 0 U1 6 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA P3.32 BP E357 EP E357 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601787 ER PT J AU Slater, G Bowsher, J Yocum, G Slater, G AF Slater, G. Bowsher, J. Yocum, G. Slater, Garett TI Nuances in diet quality and quantity influence phenotypic dimorphism during honey bee (Apis mellifera) caste determination SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 N Dakota State Univ, Fargo, ND USA. USDA ARS, Fargo, ND USA. EM garett.p.slater@ndsu.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA P1.54 BP E372 EP E372 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601845 ER PT J AU Srygley, RB AF Srygley, R. B. TI Hannibal goes for a stroll: How diet and migration can compromise immunity SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 [Srygley, R. B.] ARS, USDA, Washington, DC USA. EM robert.srygley@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA S7-4 BP E210 EP E210 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601199 ER PT J AU Zinna, RS Kraus, AL Gotoh, H Brent, CS Emlen, DJ Lavine, LS AF Zinna, R. S. Kraus, A. L. Gotoh, H. Brent, C. S. Emlen, D. J. Lavine, L. S. TI Endocrine regulation of the weapons of sexual selection SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2016 CL Portland, OR SP Soc Integrat & Comparat Biol C1 Washington State Univ, Pullman, WA 99164 USA. Gonzaga Univ, Spokane, WA 99202 USA. Nagoya Univ, Nagoya, Aichi 4648601, Japan. USDA ARS, Arid Land Agr Res Ctr, Washington, DC 20250 USA. Univ Montana, Missoula, MT 59812 USA. EM lavine@wsu.edu NR 0 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD MAR PY 2016 VL 56 SU 1 MA S6-10 BP E250 EP E250 PG 1 WC Zoology SC Zoology GA DH0FJ UT WOS:000372457601357 ER PT J AU Cole, MR Eggleston, G Borges, E Thompson, J Rathke, T Naiki, J Triplett, A AF Cole, Marsha R. Eggleston, Gillian Borges, Eduardo Thompson, Jack Rathke, Thomas Naiki, June Triplett, Alexa TI How the physical forms of starch affect filterability at a carbonatation refinery. Part I: Filterability of industrial sugars SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE filterability; total starch; viscosity; raw sugar; white sugar; insoluble starch; soluble starch; carbonatation refinery ID QUALITY; JUICE; CANE AB A study, using the USDA starch research method, was conducted to evaluate the effects of total, insoluble, and soluble starch on raw sugar filterability and viscosity. A filterability index (FI) value of 0.52 was determined using a Domino (TM) laboratory filterability method, based on the filterability of water (FI=1.00) and increasing amounts of soluble, swollen, and insoluble starch, and found to be the threshold that distinguished "good" filtering from "poor" filtering sugars. Studies on simulated affined sugar revealed that insoluble and swollen starches rapidly clogged filters, reduced filtration rates, and increased the viscosity of simulated melt liquors; whereas, soluble starch did not affect melt liquor filterability and even facilitated it. Filterability of fifty-five international raw sugars supplied by three U.S. refineries, which included two "good" and two "poor" filtering raw sugars with known carbonatation filtration performance, were also studied and compared to ICUMSA GS1-17 starch, USDA starch, % insoluble starch, ash, dextran, invert, and color. Analysis of the two "good" and two "poor" filtering raw sugars, by ICUMSA GS1-17 method, indicated that all sugars had >= 674 ppm/Brix total starch, and did not agree with their actual carbonatation filterability behaviours. These results indicated that the current 250 ppm starch limit may be too low based on the ICUMSA GS1-17 method. Using the USDA method, the "good" filtering raw sugars had <= 205 ppm/Brix total starch and <64% insoluble starch; whereas, "poor" filtering raw sugars had >= 698 ppm/Brix and >= 87% insoluble starch, and insoluble starch was strongly correlated (R=-0.969) with filterability. The FI value of 0.52 differentiated the filtering quality of the two "poor" and two "good" raw sugars and warrants more studies using raw sugars with known carbonatation performance. Raw sugar filterability is complex and may not accurately reflect actual refinery performance without consideration of carbonatation clarification reactions. Among the parameters measured, total and insoluble starch were the most detrimental to raw and white sugar filterability; however, colour, invert, dextran, and ash may be indirectly involved. More studies are needed to underpin the contribution of other insoluble and soluble impurities on the Domino filterability method and the carbonatation process. New refinery methods are urgently needed to accurately measure both soluble and insoluble starch content in raw sugars, to predict the filterability performance of raw sugars at the refinery. C1 [Cole, Marsha R.; Eggleston, Gillian; Triplett, Alexa] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Borges, Eduardo] Fermentec Ltda, Av Antonia Pazzinato Sturion 1155, BR-13420 Piracicaba, SP, Brazil. [Thompson, Jack] Louisiana Sugar Refining LLC, 1230 5th Ave, Gramercy, LA 70052 USA. [Rathke, Thomas] Imperial Sugar Co, 201 Oxnard Dr, Port Wentworth, GA 31407 USA. [Naiki, June] Amer Sugar Refining Inc, Domino Sugar Corp, 7417 N Peters St, Arabi, LA 70032 USA. RP Cole, MR (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM marsha.Cole@ars.usda.gov NR 44 TC 0 Z9 0 U1 3 U2 3 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD MAR PY 2016 VL 118 IS 1407 BP 204 EP 213 PG 10 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA DH3NC UT WOS:000372692700037 ER PT J AU Mira, S Hill, LM Gonzalez-Benito, ME Ibanez, MA Walters, C AF Mira, Sara Hill, Lisa M. Elena Gonzalez-Benito, M. Angel Ibanez, Miguel Walters, Christina TI Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Fermentation; gas chromatography; methanol; pentane; peroxidation; seed aging; seed storage; seed quality markers; volatile organic compounds; water content ID GAS-CHROMATOGRAPHIC ANALYSIS; LIPID-PEROXIDATION; PROTEIN OXIDATION; EXPIRED AIR; FATTY-ACID; STORAGE; VIABILITY; EVOLUTION; KINETICS; STRESS AB The nature and kinetics of reactions in dry seeds determines how long the seeds survive. We used gas chromatography to assay volatile organic compounds (VOCs) emitted from seeds of three unrelated species as a means to non-invasively probe chemical changes during very dry, dry, and humid storage (seeds were dried to 5.5, 33, and 75% relative humidity at room temperature). VOCs emitted from seeds stored in humid conditions reflected fermentation-type reactions, with methanol and ethanol being predominant in Lactuca sativa and Carum carvi, and acetaldehyde and acetone being predominant in Eruca vesicaria. Dried C. carvi seeds continued to emit fermentation-type products, although at slower rates than the seeds stored in humid conditions. In contrast, drying caused a switch in VOC emission in L. sativa and E. vesicaria seeds towards higher emission of pentane and hexanal, molecules considered to be byproducts from the peroxidation of polyunsaturated fatty acids. Longevity correlated best with the rate of fermentation-type reactions and appeared unrelated to the rate of lipid peroxidation. Emission of VOCs decreased when seed species were mixed together, indicating that seeds adsorbed VOCs. Adsorption of VOCs did not appear to damage seeds, as longevity was not affected in seed mixtures. Collectively, the study shows similarity among species in the types of reactions that occur in dry seeds, but high diversity in the substrates, and hence the byproducts, of the reactions. Moreover, the study suggests that the most abundant VOCs arise from degradation of storage reserves within seed cells, and that these reactions and their byproducts are not, in themselves, damaging. C1 [Mira, Sara; Elena Gonzalez-Benito, M.] Univ Politecn Madrid, Dept Biotecnol Biol Vegetal, ETSI Agronoms, Madrid, Spain. [Mira, Sara; Hill, Lisa M.; Walters, Christina] USDA ARS, Natl Ctr Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80524 USA. [Angel Ibanez, Miguel] Univ Politecn Madrid, Dept Econ Agr Estadist & Gest Empresas, ETSI Agronomos, Madrid, Spain. RP Walters, C (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80524 USA. EM christina.walters@ars.usda.gov RI Mira, Sara/C-3248-2009; Gonzalez-Benito, M. Elena/K-8314-2013 OI Mira, Sara/0000-0002-0164-5156; Gonzalez-Benito, M. Elena/0000-0002-6330-332X FU Ministerio de Educacion, Spain [CGL2006-10536]; FPU program (Ministerio de Educacion, Spain); Consejo Social (Universidad Politecnica de Madrid); Jose Castillejo program (Ministerio de Educacion, Spain) FX This work was supported by the project CGL2006-10536 (Ministerio de Educacion, Spain). SM was supported by the FPU program (Ministerio de Educacion, Spain), a grant from the Consejo Social (Universidad Politecnica de Madrid), and by the Jose Castillejo program (Ministerio de Educacion, Spain). NR 58 TC 0 Z9 0 U1 12 U2 25 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2016 VL 67 IS 6 BP 1783 EP 1793 DI 10.1093/jxb/erv568 PG 11 WC Plant Sciences SC Plant Sciences GA DH7NV UT WOS:000372981800017 PM 26956506 ER PT J AU Gassmann, W Appel, HM Oliver, MJ AF Gassmann, Walter Appel, Heidi M. Oliver, Melvin J. TI The interface between abiotic and biotic stress responses Preface SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Editorial Material C1 [Gassmann, Walter; Appel, Heidi M.] Univ Missouri, Columbia, MO 65211 USA. [Oliver, Melvin J.] USDA ARS, Washington, DC 20250 USA. RP Gassmann, W (reprint author), Univ Missouri, Columbia, MO 65211 USA. EM gassmannw@missouri.edu NR 9 TC 1 Z9 1 U1 6 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAR PY 2016 VL 67 IS 7 BP 2023 EP 2024 DI 10.1093/jxb/erw110 PG 2 WC Plant Sciences SC Plant Sciences GA DH7NY UT WOS:000372982100001 PM 27433565 ER PT J AU Hwang, IH Swenson, DC Gloer, JB Wicklow, DT AF Hwang, In Hyun Swenson, Dale C. Gloer, James B. Wicklow, Donald T. TI Disseminins and Spiciferone Analogues: Polyketide-Derived Metabolites from a Fungicolous Isolate of Pestalotiopsis disseminata SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID FUNGUS COCHLIOBOLUS-SPICIFER; BIOACTIVE METABOLITES; NATURAL-PRODUCTS; SESQUITERPENOIDS; BIOSYNTHESIS; ASPERGILLUS AB Seven new polyketide metabolites (disseminins A-E, 1-5, and spiciferones D and E, 7 and 8) were obtained from cultures of a fungicolous isolate of Pestalotiopsis disseminata (NRRL 62562), together with a related compound (6) previously known only as a semisynthetic product. Structures were determined mainly by analysis of HRMS and NMR data. Biogenetically related compounds 1 and 2 possess uncommon bis-tetrahydrofuran and dioxabicyclo[3.2.1]octane ring systems, respectively. X-ray crystallographic analysis of the p-bromobenzoate derivative of 1 confirmed the structure and enabled assignment of its absolute configuration. C1 [Hwang, In Hyun; Swenson, Dale C.; Gloer, James B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, Donald T.] Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, ARS, USDA, Peoria, IL 61604 USA. RP Gloer, JB (reprint author), Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. EM james-gloer@uiowa.edu FU National Science Foundation [CHE-1011847]; National Institutes of Health [S10 RR025500, S10 RR023384]; University of Iowa FX Financial support was provided in part by a grant from the National Science Foundation (CHE-1011847). Acquisition of NMR and MS instrumentation employed in this work was supported by grants from the National Institutes of Health (S10 RR025500 and S10 RR023384, respectively) and by University of Iowa funds. Technical assistance from staff of the University of Iowa NMR and MS facilities is greatly appreciated. The USDA is an equal opportunity provider and employer. NR 17 TC 2 Z9 2 U1 1 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD MAR PY 2016 VL 79 IS 3 BP 523 EP 530 DI 10.1021/acs.jnatprod.5b00907 PG 8 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA DH8GJ UT WOS:000373031200011 PM 26641923 ER PT J AU Killeen, DP Larsen, L Dayan, FE Gordon, KC Perry, NB van Klink, JW AF Killeen, Daniel P. Larsen, Lesley Dayan, Franck E. Gordon, Keith C. Perry, Nigel B. van Klink, John W. TI Nortriketones: Antimicrobial Trimethylated Acylphloroglucinols from Manuka (Leptospermum scoparium) SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID NEW-ZEALAND MANUKA; MYRICA-GALE L; P-HYDROXYPHENYLPYRUVATE DIOXYGENASE; C-METHYLATED DIHYDROCHALCONES; BETA-TRIKETONES; ESSENTIAL OILS; QUANTIFICATION; METHYLGLYOXAL; MYRTACEAE AB Four trimethylated acylphloroglucinols (5-8) have been isolated from ircaanika (Leptospermum scoparium) foliage. Apart from myrigalone A (8), which has previously been isolated from European bog myrtle (Myrica gale), these compounds have not been characterized before. The nortriketones are structurally similar to the bioactive tetramethylated beta-triketones from rranuka, but have one less ring methyl group. Two oxidized trimethylated compounds, 9 and 10, were also isolated, but these are likely isolation artifacts. 'When evaluated for antibacterial activity against Gram-positive bacteria, myrigalone A (8) was slightly less potent (MIC 64 mu g/mL) than the corresponding tetramethylated compound, grandiflorone (4) (MIC 16-32 mu g/mL). Unlike their tetramethylated analogues, the nortriketones were inactive against the herbicide target enzyme p-hydroxyphenylpyruvate dioxygenase. The Raman spectra of leaf oil glands in different manuka varieties can be used to distinguish plants that contain nortriketones from those that accumulate triketones. C1 [Killeen, Daniel P.; Larsen, Lesley; Gordon, Keith C.; Perry, Nigel B.; van Klink, John W.] Univ Otago, Dept Chem, POB 56, Dunedin, New Zealand. [Killeen, Daniel P.; Larsen, Lesley; Perry, Nigel B.; van Klink, John W.] Univ Otago, New Zealand Inst Plant & Food Res Ltd, POB 56, Dunedin, New Zealand. [Dayan, Franck E.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Dayan, Franck E.] Colorado State Univ, Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. RP van Klink, JW (reprint author), Univ Otago, Dept Chem, POB 56, Dunedin, New Zealand.; van Klink, JW (reprint author), Univ Otago, New Zealand Inst Plant & Food Res Ltd, POB 56, Dunedin, New Zealand. EM john.vanklink@plantandfood.co.nz RI Perry, Nigel/G-5574-2010; OI Perry, Nigel/0000-0003-3196-3945; Dayan, Franck/0000-0001-6964-2499; Gordon, Keith/0000-0003-2833-6166 FU University of Otago; Dodd-Walls Centre FX We thank B. Smallfield for the identification and cultivation of the L. scoparium plant material, J. Pentelow for assistance with MS, I. Stewart for assistance with NMR, G. Cook for antibacterial assays, and S. B. Watson for HPPD assays. This research was supported by a University of Otago Doctoral Scholarship to D.P.K. K.C.G. thanks the Dodd-Walls Centre for support. NR 21 TC 3 Z9 3 U1 9 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD MAR PY 2016 VL 79 IS 3 BP 564 EP 569 DI 10.1021/acs.jnatprod.5b00968 PG 6 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA DH8GJ UT WOS:000373031200015 PM 26731565 ER PT J AU Ghimire, MN Arthur, FH Myers, SW Phillips, TW AF Ghimire, Mukti N. Arthur, Frank H. Myers, Scott W. Phillips, Thomas W. TI Residual efficacy of deltamethrin and beta-cyfluthrin against Trogoderma variabile and Trogoderma inclusum (Coleoptera: Dermestidae) SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Stored product insects; Insecticides; Dermestids; Quarantine pest; Trogoderma ID TRIBOLIUM-CONFUSUM COLEOPTERA; GRANARIUM EVERTS COLEOPTERA; METHYL PLUS DELTAMETHRIN; STORED-PRODUCT INSECTS; WETTABLE POWDER; PYRETHRIN AEROSOL; FIELD STRAINS; FOOD SOURCE; CONCRETE; TENEBRIONIDAE AB Trogroderma variabile Ballion, warehouse beetle, and Trogoderma inclusum LeConte, larger cabinet beetle, are dermestid pests of stored products. A series of laboratory bioassays were conducted to evaluate residual toxicity of the pyrethroids deltamethrin and beta-cyfluthrin, applied on a concrete surface substrate for control of adults and larvae of both species, to provide initial baseline susceptibility data for dermestids. Commercial formulations were applied at calculated deposition rates of 8, 16, and 24 mg active ingredient [AI] per m(2) for deltamethrin and 10 and 20 mg active ingredient [AI] per m(2) for beta-cyfluthrin. Ten adults or larvae of either species were introduced to individual untreated and treated arenas at 0, 0.5, 1, 2, and 3 mo post-treatment Adult mortality was evaluated after 4 d of exposure with no food provided, and larval mortality was assessed 30 d after exposure with provision of food. Adult mortality of both species exposed to both insecticides ranged from 72.4 +/- 6.9 to 100% depending on exposure interval. Larval mortality ranged from 183 +/- 5.4 to 96.7 +/- 2.1% on arenas treated with deltamethrin, and was significantly lower than adult mortality (P < 0.001) at bioassays conducted at 2 and 3 mo post-treatment for both species at all three rates. Larval mortality on arenas treated with beta-cyfluthrin ranged from 20.0 +/- 4.5 to 71.7 +/- 4.8%, and was always lower (P < 0.001) at all exposure times at both rates for both species. There were only 4 out of a possible 20 comparisons with a significant difference (P < 0.05) between the three rates of deltamethrin and no occasions where there is a significant difference in mortality between the two rates of beta-cyfluthrin. There were three occasions where mortality of T. inclusum adults or larvae was greater than T variabile, and one occasion with the reverse, for exposures with deltamethrin. Results show larvae of both species were more tolerant than adults of both species, and larvae could be used as an indicator stage for future studies insecticidal susceptibility of Dermestids. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Ghimire, Mukti N.; Phillips, Thomas W.] Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA. [Ghimire, Mukti N.; Myers, Scott W.] US Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Ctr Plant Hlth Sci & Technol, 1398 West Truck Rd, Buzzards Bay, MA 02542 USA. [Arthur, Frank H.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Ghimire, MN (reprint author), Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA. EM Mukti.N.Ghimire@aphis.usda.gov FU USDA Farm Bill Section [6.0255.01]; Kansas Agricultural Experiment Station [16-058-J] FX We thank BASF Corporation for providing the deltamethrin and Bayer Corporation for providing the beta-cyfluthrin used in this study. Hannah Lewis-Rosenblum helped preparation of experimental arenas and applying treatments. Mention of trade names or commercial products used in this publication is solely for the purpose of providing scientific information and does not imply recommendation or constitute an endorsement for its use by the U.S. Department of Agriculture or Kansas State University. The USDA and Kansas State University are equal opportunity providers and employers. This project was funded through USDA Farm Bill Section 10201 (project number: 6.0255.01) and was partially funded by the Kansas Agricultural Experiment Station, for which this article represents paper no. 16-058-J. NR 33 TC 1 Z9 1 U1 3 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD MAR PY 2016 VL 66 BP 6 EP 11 DI 10.1016/j.jspr.2015.12.002 PG 6 WC Entomology SC Entomology GA DH4OH UT WOS:000372764800002 ER PT J AU Fitzgerald, SD Hollinger, C Mullaney, TP Bruning-Fann, CS Tilden, J Smith, R Averill, J Kaneene, JB AF Fitzgerald, Scott D. Hollinger, Charlotte Mullaney, Thomas P. Bruning-Fann, Colleen S. Tilden, John Smith, Rick Averill, James Kaneene, John B. TI Herd outbreak of bovine tuberculosis illustrates that route of infection correlates with anatomic distribution of lesions in cattle and cats SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Bovine tuberculosis; cattle; domestic cats; epidemiology; pathology ID MYCOBACTERIUM-BOVIS; MILK AB An outbreak of bovine tuberculosis (TB) in a Michigan dairy herd resulted in quarantine, depopulation, pathology, and epidemiologic investigations. This herd, compared to other TB-infected herds in Michigan, was unusual in the long-term feeding of waste milk to its replacement calves. The herd had 80 cattle with positive results on caudal fold test or gamma interferon testing, which were reclassified as suspects because the herd had never been known to be tuberculous previously. Autopsy revealed striking variation in the anatomic distribution of gross anatomic lesions, microscopic lesions, and culture-positive lymph nodes between the adult cattle, the calves, and the domestic cats present on the farm. Adult cattle had lesions and culture-positive lymph nodes predominantly within the thoracic lymph nodes, whereas cats had 50% of their lesions and culture-positive lymph nodes in their abdomens, and 50% of positive calves had culture-positive lymph nodes in their abdomens. This difference in anatomic distribution correlated with the likely routes of infection, which are believed to be by direct airborne transmission in adult cattle and indirect ingestion of contaminated milk in both calves and cats. Although TB literature over the past 100-plus years states that the route of infection may manifest itself in differences in lesion anatomic distribution, our team has been working with TB for over 20 years, and we have never encountered such striking variation between different groups of animals on the same farm. C1 [Fitzgerald, Scott D.; Hollinger, Charlotte; Mullaney, Thomas P.] Michigan State Univ, Diagnost Ctr Populat & Anim Hlth, 4125 Beaumont Rd, Lansing, MI 48910 USA. [Bruning-Fann, Colleen S.] Anim Plant Hlth Inspect Serv, USDA, Dist Off, E Lansing, MI USA. [Tilden, John] Michigan Dept Agr & Rural Dev, Food Safety Planning & Response Unit, Lansing, MI USA. [Smith, Rick; Averill, James] Michigan Dept Agr & Rural Dev, State Veterinarians Off, Lansing, MI USA. [Kaneene, John B.] Michigan State Univ, Ctr Comparat Epidemiol, E Lansing, MI 48824 USA. RP Fitzgerald, SD (reprint author), Michigan State Univ, Diagnost Ctr Populat & Anim Hlth, 4125 Beaumont Rd, Lansing, MI 48910 USA. EM fitzgerald@dcpah.msu.edu NR 11 TC 0 Z9 0 U1 3 U2 10 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD MAR PY 2016 VL 28 IS 2 BP 129 EP 132 DI 10.1177/1040638715626484 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA DH5HH UT WOS:000372816600007 PM 26965232 ER PT J AU Sullivan, LA Wakayama, J Boscan, PL Hyatt, DR Twedt, DC Lappin, MR Dargatz, DA AF Sullivan, Lauren A. Wakayama, Justin Boscan, Pedro L. Hyatt, Doreene R. Twedt, David C. Lappin, Michael R. Dargatz, David A. TI The effects of omeprazole therapy on bacterial colonization of the pharynx in healthy dogs SO JOURNAL OF VETERINARY EMERGENCY AND CRITICAL CARE LA English DT Article DE antacids; dysbiosis; proton pump inhibitors ID GRAM-NEGATIVE BACILLI; NOSOCOMIAL PNEUMONIA; GASTRIC COLONIZATION; FLORA AB ObjectiveTo identify the relative abundance of commensal pharyngeal flora in healthy dogs and determine if abundance of pharyngeal flora is altered during omeprazole administration. AnimalsEight adult Beagles. ProceduresA total of 3 baseline pharyngeal swabs, collected 48 hours apart, were obtained from each dog. Omeprazole (1 mg/kg PO q 24 h) was administered for a total of 12 days. During omeprazole administration, pharyngeal swabs were obtained on Days 8, 10, and 12. All swabs were submitted for semiquantitative aerobic and anaerobic culture. Growth of bacterial isolates, as well as genus of isolates, was compared between the pretreatment (n = 24) and treatment (n = 24) swabs. ResultsA greater abundance of several bacterial species was identified during the treatment period, including coagulase-negative Staphylococcus (P < 0.01), Bacillus (P < 0.01), and Pasteurella (P = 0.05). The abundance of bacterial species in samples collected during the treatment period was unchanged for Escherichia coli (P = 0.16), Provotella (P = 0.40), hemolytic Streptococcus (P = 0.34), and nonhemolytic Streptococcus (P = 0.14). Conclusions and Clinical RelevanceThis small study indicates that shifts in canine pharyngeal flora may occur during omeprazole therapy. Further studies are warranted to determine the clinical significance of gastric acid suppressants on pharyngeal flora in dogs. C1 [Sullivan, Lauren A.; Wakayama, Justin; Boscan, Pedro L.; Twedt, David C.; Lappin, Michael R.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Clin Sci, 300 West Drake Rd, Ft Collins, CO 80523 USA. [Hyatt, Doreene R.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, 300 West Drake Rd, Ft Collins, CO 80523 USA. [Dargatz, David A.] USDA APHIS, VS, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave Bldg B, Ft Collins, CO 80526 USA. RP Sullivan, LA (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Clin Sci, 300 West Drake Rd, Ft Collins, CO 80523 USA. EM lauren.sullivan@colostate.edu RI Hyatt, Doreene/E-6811-2017 FU Center for Companion Animal Studies PVM student grant program at Colorado State University FX Supported in part by the Center for Companion Animal Studies PVM student grant program at Colorado State University. NR 12 TC 1 Z9 1 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1479-3261 EI 1476-4431 J9 J VET EMERG CRIT CAR JI J. Vet. Emerg. Crit. Care PD MAR-APR PY 2016 VL 26 IS 2 BP 300 EP 304 DI 10.1111/vec.12432 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DI0ND UT WOS:000373192400010 PM 26645977 ER PT J AU Lee, DH Kwon, JH Noh, JY Park, JK Yuk, SS Erdene-Ochir, TO Nahm, SS Kwon, YK Lee, SW Song, CS AF Lee, Dong-Hun Kwon, Jung-Hoon Noh, Jin-Yong Park, Jae-Keun Yuk, Seong-Su Erdene-Ochir, Tseren-Ochir Nahm, Sang-Soep Kwon, Yong-Kuk Lee, Sang-Won Song, Chang-Seon TI Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens SO JOURNAL OF VETERINARY SCIENCE LA English DT Article DE chicken; feathers; Newcastle disease virus ID H5N1 AB Newcastle disease viruses (NDVs) cause systemic diseases in chickens with high mortality. However, little is known about persistence of NDVs in contaminated tissues from infected birds. In this study, we examined viral replication in the feather pulp of chickens inoculated with viscerotropic velogenic NDV (vvNDV) genotype VII. Reverse transcription real-time PCR and immunohistochemistry were used to investigate viral persistence in the samples. vvNDV was detected in the oropharynx and cloaca and viral antigens were detected in the feathers, suggesting that feathers act as sources of viral transmission. C1 [Lee, Dong-Hun; Kwon, Jung-Hoon; Noh, Jin-Yong; Park, Jae-Keun; Yuk, Seong-Su; Erdene-Ochir, Tseren-Ochir; Lee, Sang-Won; Song, Chang-Seon] Konkuk Univ, Coll Vet Med, Avian Dis Lab, Seoul 05029, South Korea. [Nahm, Sang-Soep] Konkuk Univ, Coll Vet Med, Lab Vet Anat, Seoul 05029, South Korea. [Kwon, Yong-Kuk] Minist Agr Food & Rural Affairs, Anim & Plant Quarantine Agcy, Anyang 14089, South Korea. [Lee, Dong-Hun] USDA, Southeast Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. RP Song, CS (reprint author), Konkuk Univ, Coll Vet Med, Avian Dis Lab, Seoul 05029, South Korea. EM songcs@konkuk.ac.kr FU Agricultural Biotechnology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [314017-3] FX This research was supported by the Agricultural Biotechnology Development Program (grant No. 314017-3), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. The primary antibody against the nucleoprotein for IHC was kindly provided by Dr. Claudio Afonso, USDA Southeast Poultry Research Laboratory, USA. NR 8 TC 0 Z9 0 U1 1 U2 1 PU KOREAN SOC VETERINARY SCIENCE PI SEOUL PA SEOUL NATL UNIV, COLLEGE VETERINARY MEDICINE,, SEOUL, 151-742, SOUTH KOREA SN 1229-845X EI 1976-555X J9 J VET SCI JI J. Vet. Sci. PD MAR PY 2016 VL 17 IS 1 BP 115 EP 117 DI 10.4142/jvs.2016.17.1.115 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA DI1HJ UT WOS:000373246900015 PM 27051348 ER PT J AU Gennari, SM Niemeyer, C Catao-Dias, JL Soares, HS Acosta, ICL Dias, RA Ribeiro, JD Lassalvia, C Maracini, P Kolesnikovas, CKM Mayorga, LFSP Dubey, JP AF Gennari, Solange M. Niemeyer, Claudia Catao-Dias, Jose L. Soares, Herbert S. Acosta, Igor C. L. Dias, Ricardo A. Ribeiro, Jessica D. Lassalvia, Cristiane Maracini, Pryscilla Kolesnikovas, Cristiane K. M. Mayorga, Luis F. S. P. Dubey, Jitender P. TI SURVEY OF TOXOPLASMA GONDII ANTIBODIES IN MAGELLANIC PENGUINS (SPHENISCUS MAGELLANICUS FORSTER, 1781) SO JOURNAL OF ZOO AND WILDLIFE MEDICINE LA English DT Article DE Toxoplasma gondii; Magellanic penguins; Spheniscus magellanicus; Brazilian coast ID OOCYSTS AB Magellanic penguins (Spheniscus magellanicus) breed on the Atlantic and Pacific coasts of the southernmost parts of South America and migrate northward as far as Peru and Brazil. Serum samples (n = 100) from Magellanic penguins from three zoos and two rehabilitation centers (RCs) in Brazil were assayed for the presence of antibodies to Toxoplasma gondii by means of the modified agglutination test (MAT, cut-off >= 20). The penguins were categorized as young (<= 4 yr old) or adults (>= 4 yr old) and sexed (male, female, or not identified), and data were analyzed using the chi-square test (P <= 0.05). Toxoplasma gondii antibodies were found in 28% of penguins: 25.8% males, 27.8% females, 30.3% unknown sex, 25.4% young, and 31.1% adults. Statistical analyses did not find any difference (P. 0.05) with respect to age, sex, or source of birds. This is the first report of T. gondii antibodies in S. magellanicus. C1 [Gennari, Solange M.; Soares, Herbert S.; Acosta, Igor C. L.; Dias, Ricardo A.] Univ Sao Paulo, Fac Vet Med FMVZ, Dept Prevent Vet Med & Anim Hlth, Ave Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. [Niemeyer, Claudia; Catao-Dias, Jose L.] Univ Sao Paulo, FMVZ, Dept Pathol, Ave Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. [Ribeiro, Jessica D.] Municipal Author Santo Andre, Sabina Escola Parque Conhecimento, BR-0918522 Sao Paulo, SP, Brazil. [Lassalvia, Cristiane] Municipal Aquarium Santos, Ave Bartolomeu de Gusmao, BR-11030600 Santos, SP, Brazil. [Maracini, Pryscilla] Aqua Mundo, Ave Miguel Stefano 200, BR-11440532 Guaruja, SP, Brazil. [Kolesnikovas, Cristiane K. M.] R3 Anim Assoc FATMA, Rodovia Joao Gualberto Soares, BR-88058300 Florianopolis, SC, Brazil. [Mayorga, Luis F. S. P.] Inst Res & Rehabil Marine Anim, Rodovia BR 262,Km 0, BR-29140130 Cariacica, ES, Brazil. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab,BARC East, Bldg 1001, Beltsville, MD 20705 USA. RP Gennari, SM (reprint author), Univ Sao Paulo, Fac Vet Med FMVZ, Dept Prevent Vet Med & Anim Hlth, Ave Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. EM sgennari@usp.br RI Catao-Dias, Jose Luiz/C-4897-2012 OI Catao-Dias, Jose Luiz/0000-0003-2999-3395 FU National Research Council (CNPq), Brazil FX SMG, RAD, and JLCD received fellowships from the National Research Council (CNPq), Brazil. NR 12 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC ZOO VETERINARIANS PI YULEE PA 581705 WHITE OAK ROAD, YULEE, FL 32097 USA SN 1042-7260 EI 1937-2825 J9 J ZOO WILDLIFE MED JI J. Zoo Wildl. Med. PD MAR PY 2016 VL 47 IS 1 BP 364 EP 366 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA DI0TU UT WOS:000373211000047 PM 27010304 ER PT J AU Kilkenny, FF Galloway, LF AF Kilkenny, Francis F. Galloway, Laura F. TI Evolution of marginal populations of an invasive vine increases the likelihood of future spread SO NEW PHYTOLOGIST LA English DT Article DE adaptive evolution; climate change; common garden; contemporary evolution; invasive species; range expansion; response functions ID CLIMATE-CHANGE; RANGE-EXPANSION; RAPID EVOLUTION; BIOLOGICAL INVASIONS; PINUS-CONTORTA; INVADED RANGE; NORTH-AMERICA; SPECIES RANGE; PLANT; ADAPTATION AB The prediction of invasion patterns may require an understanding of intraspecific differentiation in invasive species and its interaction with climate change. We compare Japanese honeysuckle (Lonicera japonica) plants from the core (100-150 yr old) and northern margin (< 65 yr old) of their North American invaded range to determine whether evolution during invasion increases the probability of future expansion. Plants from populations in the core and margin were compared in two sites beyond the northern range edge to assess their potential to invade novel areas. Data were compared with previous work to assess the effect of latitudinal climate on L. japonica spread. Winter survival in current climates was modeled and projected for future climates to predict future spread. Margin plants were larger and had 60% greater survival than core plants at sites beyond the northern range edge. Overall, winter survival decreased with increasing latitude and decreasing temperature, and was greater in margin plants than core plants. Models suggested that greater winter tolerance in margin populations has increased L. japonica's northward spread by 76 km, and that this survival advantage will persist under future climates. These results demonstrate that evolution during invasion may increase spread beyond predictions using increasing global temperatures alone. C1 [Kilkenny, Francis F.; Galloway, Laura F.] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA. [Kilkenny, Francis F.] US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID 83702 USA. RP Kilkenny, FF (reprint author), Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA.; Kilkenny, FF (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID 83702 USA. EM ffkilkenny@fs.fed.us FU Jeffress Foundation FX We wish to thank G. H. Gordon Biological Station, run by Hillsdale College, Hillsdale, MI, USA, and the University of Wisconsin at Milwaukee Field Station, Saukville, WI, USA for providing field sites. We are also grateful to J. Reinartz and A. Verhoef for help in the field, K. Burgess and E. Yoshizuka for help in the glasshouse, the University of Virginia for glasshouse space, W. Crannage for keeping the glasshouse running smoothly, the Jeffress Foundation for funding, and F. Kilkenny's committee members for comments on previous drafts. NR 65 TC 0 Z9 0 U1 18 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD MAR PY 2016 VL 209 IS 4 BP 1773 EP 1780 DI 10.1111/nph.13702 PG 8 WC Plant Sciences SC Plant Sciences GA DI3DY UT WOS:000373379800041 PM 26467337 ER PT J AU Dubey, JP Laurin, E Kwowk, OCH AF Dubey, J. P. Laurin, E. Kwowk, O. C. H. TI Validation of the modified agglutination test for the detection of Toxoplasma gondii in free-range chickens by using cat and mouse bioassay SO PARASITOLOGY LA English DT Article DE Toxoplasma gondii; chickens; modified agglutination test; bioassay ID MOLECULAR CHARACTERIZATION; GENETIC-CHARACTERISTICS; TISSUE DISTRIBUTION; BIOLOGIC CHARACTERISTICS; UNEXPECTED FINDINGS; GALLUS-DOMESTICUS; HIGH PREVALENCE; CENTRAL-AMERICA; SOUTH-AMERICA; UNITED-STATES AB The modified agglutination test (MAT) is one of the most commonly used tests for the detection of antibodies to Toxoplasma gondii in animal and human sera. The objective of the present study was to evaluate the diagnostic accuracy of the MAT and bioassay in free-range/backyard (FR) chickens (Gallus domesticus). Previously-published T. gondii test results from 2066 chickens from 19 countries were compiled for the present study. The frequency of isolation of T. gondii increased for MAT titres between 1:5 and >= 1:160, and ranged from 61 to 75% for antibody titres of 1:160, 1:320, and 1:640. Twenty-three cats fed pooled hearts from a total of 802 FR seronegative (MAT, <1:5) chickens from several countries did not excrete oocysts, indicating a high negative predictive value of MAT because FR chickens would have been exposed to many microbes; cats are the most sensitive indicators of T. gondii infection in tissues and can excrete millions of oocysts after ingesting even a few bradyzoites. Of the 29 cats in this study, six cats, fed hearts pooled from 15-122 FR chickens, excreted oocysts; but these identifications were likely related to misidentification or prozone. Results of the present study support the validity of MAT for the detection of T. gondii infection in chickens. C1 [Dubey, J. P.; Kwowk, O. C. H.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, BARC East,Bldg 1001, Beltsville, MD 20705 USA. [Laurin, E.] Atlantic Vet Coll, Dept Hlth Management, 550 Univ Ave, Charlottetown, PE C1A 4P3, Canada. RP Dubey, JP (reprint author), USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, BARC East,Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 38 TC 12 Z9 12 U1 3 U2 8 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD MAR PY 2016 VL 143 IS 3 BP 314 EP 319 DI 10.1017/S0031182015001316 PG 6 WC Parasitology SC Parasitology GA DH5HD UT WOS:000372816200006 PM 26625933 ER PT J AU Bhowmik, S Chiu, HP Jones, DH Chiu, HJ Miller, MD Xu, QP Farr, CL Ridlon, JM Wells, JE Elsliger, MA Wilson, IA Hylemon, PB Lesley, SA AF Bhowmik, Shiva Chiu, Hsien-Po Jones, David H. Chiu, Hsiu-Ju Miller, Mitchell D. Xu, Qingping Farr, Carol L. Ridlon, Jason M. Wells, James E. Elsliger, Marc-Andre Wilson, Ian A. Hylemon, Phillip B. Lesley, Scott A. TI Structure and functional characterization of a bile acid 7 alpha dehydratase BaiE in secondary bile acid synthesis SO PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS LA English DT Article DE gut microbes; secondary bile acid synthesis; gut microbe mediated human metabolite; 7 alpha-dehyroxylation; bile acid 7 alpha-dehydratase; primary bile acid; secondary bile acid; nuclear transport factor-2 superfamily; structural genomics ID SITE-DIRECTED MUTAGENESIS; SP STRAIN VPI-12708; SCYTALONE DEHYDRATASE; CLOSTRIDIUM-SCINDENS; STRUCTURE VALIDATION; CRYSTAL-STRUCTURES; ESCHERICHIA-COLI; DEOXYCHOLIC-ACID; NUCLEIC-ACIDS; VPI 12708 AB Conversion of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) to the secondary bile acids deoxycholic acid (DCA) and lithocholic acid (LCA) is performed by a few species of intestinal bacteria in the genus Clostridium through a multistep biochemical pathway that removes a 7 alpha-hydroxyl group. The rate-determining enzyme in this pathway is bile acid 7 alpha-dehydratase (baiE). In this study, crystal structures of apo-BaiE and its putative product-bound [3-oxo-Delta(4,6)-lithocholyl-Coenzyme A (CoA)] complex are reported. BaiE is a trimer with a twisted alpha + beta barrel fold with similarity to the Nuclear Transport Factor 2 (NTF2) superfamily. Tyr30, Asp35, and His83 form a catalytic triad that is conserved across this family. Site-directed mutagenesis of BaiE from Clostridium scindens VPI 12708 confirm that these residues are essential for catalysis and also the importance of other conserved residues, Tyr54 and Arg146, which are involved in substrate binding and affect catalytic turnover. Steady-state kinetic studies reveal that the BaiE homologs are able to turn over 3-oxo-Delta(4)-bile acid and CoA-conjugated 3-oxo-Delta(4)-bile acid substrates with comparable efficiency questioning the role of CoA-conjugation in the bile acid metabolism pathway. C1 [Bhowmik, Shiva; Chiu, Hsiu-Ju; Miller, Mitchell D.; Xu, Qingping; Farr, Carol L.; Elsliger, Marc-Andre; Wilson, Ian A.; Lesley, Scott A.] Joint Ctr Struct Gen, La Jolla, CA USA. [Bhowmik, Shiva; Farr, Carol L.; Elsliger, Marc-Andre; Wilson, Ian A.; Lesley, Scott A.] Scripps Res Inst, Dept Integrat Struct & Computat Biol, 10550 North Torrey Pines Rd, La Jolla, CA 92037 USA. [Chiu, Hsien-Po; Jones, David H.; Lesley, Scott A.] Genom Inst Novartis Res Fdn, 10675 John Jay Hopkins Dr, San Diego, CA 92121 USA. [Chiu, Hsiu-Ju; Miller, Mitchell D.; Xu, Qingping] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, 2575 Sand Hill Rd,MS 9, Menlo Pk, CA 94025 USA. [Ridlon, Jason M.; Hylemon, Phillip B.] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23298 USA. [Ridlon, Jason M.; Hylemon, Phillip B.] McGuire VA Med Ctr, Richmond, VA 23298 USA. [Wells, James E.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Lesley, SA (reprint author), Scripps Res Inst, Dept Integrat Struct & Computat Biol, 10550 North Torrey Pines Rd, La Jolla, CA 92037 USA. EM slesley@scripps.edu FU U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-76SF00515]; DOE Office of Biological and Environmental Research; National Institutes of Health, National Institute of General Medical Sciences [P41GM103393]; Director, Office of Science, Office of Basic Energy Sciences, Materials Sciences Division of the US Department of Energy at Lawrence Berkeley National Laboratory [DE-AC02-05CH11231]; NIH; NIGMS; Howard Hughes Medical Institute FX We thank the members of the JCSG high-throughput structural biology pipeline for their contribution to this work. Portions of this research were carried out at the Stanford Synchrotron Radiation Lightsource (SSRL) and the Advanced Light Source (ALS). Use of the Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. The SSRL Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research, and by the National Institutes of Health, National Institute of General Medical Sciences (including P41GM103393). The ALS is supported by the Director, Office of Science, Office of Basic Energy Sciences, Materials Sciences Division of the US Department of Energy under Contract No. DE-AC02-05CH11231 at Lawrence Berkeley National Laboratory. The Berkeley Center for Structural Biology is supported in part by NIH, NIGMS and the Howard Hughes Medical Institute. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the NIGMS or the NIH. NR 60 TC 3 Z9 3 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0887-3585 EI 1097-0134 J9 PROTEINS JI Proteins PD MAR PY 2016 VL 84 IS 3 BP 316 EP 331 DI 10.1002/prot.24971 PG 16 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DI2TP UT WOS:000373352100003 PM 26650892 ER PT J AU Xu, XW Yu, T Xu, RX Shi, Y Lin, XJ Xu, Q Qi, XH Weng, YQ Chen, XH AF Xu, Xuewen Yu, Ting Xu, Ruixue Shi, Yang Lin, Xiaojian Xu, Qiang Qi, Xiaohua Weng, Yiqun Chen, Xuehao TI Fine mapping of a dominantly inherited powdery mildew resistance major-effect QTL, Pm1.1, in cucumber identifies a 41.1 kb region containing two tandemly arrayed cysteine-rich receptor-like protein kinase genes SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID SATIVUS L.; ARABIDOPSIS; LOCI; TRAITS; STRESS; GENOME; LINES AB Key message A dominantly inherited major-effect QTL for powdery mildew resistance in cucumber was fine mapped. Two tandemly arrayed cysteine-rich receptor-like protein kinase genes were identified as the most possible candidates. Powdery mildew (PM) is one of the most severe fungal diseases of cucumber (Cucumis sativus L.) and other cucurbit crops, but the molecular genetic mechanisms of powdery mildew resistance in cucurbits are still poorly understood. In this study, through marker-assisted backcrossing with an elite cucumber inbred line, D8 (PM susceptible), we developed a single-segment substitution line, SSSL0.7, carrying 95 kb fragment from PM resistance donor, Jin5-508, that was defined by two microsatellite markers, SSR16472 and SSR16881. A segregating population with 3600 F-2 plants was developed from the SSSL0.7 x D8 mating; segregation analysis confirmed a dominantly inherited major-effect QTL, Pm1.1 in cucumber chromosome 1 underlying PM resistance in SSSL0.7. New molecular markers were developed through exploring the next generation resequenced genomes of Jin5-508 and D8. Linkage analysis and QTL mapping in a subset of the F-2 plants delimited the Pm1.1 locus into a 41.1 kb region, in which eight genes were predicted. Comparative gene expression analysis revealed that two concatenated genes, Csa1M064780 and Csa1M064790 encoding the same function of a cysteine-rich receptor-like protein kinase, were the most likely candidate genes. GFP fusion protein-aided subcellular localization indicated that both candidate genes were located in the plasma membrane, but Csa1M064780 was also found in the nucleus. This is the first report of dominantly inherited PM resistance in cucumber. Results of this study will provide new insights into understanding the phenotypic and genetic mechanisms of PM resistance in cucumber. This work should also facilitate marker-assisted selection in cucumber breeding for PM resistance. C1 [Xu, Xuewen; Yu, Ting; Xu, Ruixue; Shi, Yang; Lin, Xiaojian; Xu, Qiang; Qi, Xiaohua; Chen, Xuehao] Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. [Weng, Yiqun] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Dept Hort, Madison, WI 53706 USA. RP Chen, XH (reprint author), Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China.; Weng, YQ (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Dept Hort, Madison, WI 53706 USA. EM yiqun.weng@ars.usda.gov; xhchen@yzu.edu.cn FU National Natural Science Foundation of China [31171978]; National Key Basic Research Program of China (973 Program) [2012CB113900]; National Science & Technology Project for Rural Area [2012AA100103]; International Foundation for Science [C/5150-1]; Jiangsu Science & Technology Project [BE2012326] FX This work was supported by the National Natural Science Foundation of China (No. 31171978), the National Key Basic Research Program of China (973 Program) (No. 2012CB113900), National Science & Technology Project for Rural Area (No. 2012AA100103), International Foundation for Science (No. C/5150-1) and Jiangsu Science & Technology Project (BE2012326). NR 39 TC 3 Z9 3 U1 8 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2016 VL 129 IS 3 BP 507 EP 516 DI 10.1007/s00122-015-2644-4 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DH6LX UT WOS:000372903200005 PM 26660669 ER PT J AU King, ZR Harris, DK Pedley, KF Song, QJ Wang, DC Wen, ZX Buck, JW Li, ZL Boerma, HR AF King, Zachary R. Harris, Donna K. Pedley, Kerry F. Song, Qijian Wang, Dechun Wen, Zixiang Buck, James W. Li, Zenglu Boerma, H. Roger TI A novel Phakopsora pachyrhizi resistance allele (Rpp) contributed by PI 567068A SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID ASIAN SOYBEAN RUST; BULKED SEGREGANT ANALYSIS; UNITED-STATES; CONFERS RESISTANCE; CANDIDATE GENES; IDENTIFICATION; REGISTRATION; LOCUS; CONFIRMATION; POPULATIONS AB Key message The Rpp6 locus of PI 567102B was mapped from 5,953,237 to 5,998,461 bp (chromosome 18); and a novel allele at the Rpp6 locus or tightly linked gene Rpp[PI567068A] of PI 567068A was mapped from 5,998,461 to 6,160,481 bp. Soybean rust (SBR), caused by the obligate, fungal pathogen Phakopsora pachyrhizi is an economic threat to soybean production, especially in the Americas. Host plant resistance is an important management strategy for SBR. The most recently described resistance to P. pachyrhizi (Rpp) gene is Rpp6 contributed by PI 567102B. Rpp6 was previously mapped to an interval of over four million base pairs on chromosome 18. PI 567068A was recently demonstrated to possess a resistance gene near the Rpp6 locus, yet PI 567068A gave a differential isolate reaction to several international isolates of P. pachyrhizi. The goals of this research were to fine map the Rpp6 locus of PI 567102B and PI 567068A and determine whether or not PI 567068A harbors a novel Rpp6 allele or another allele at a tightly linked resistance locus. Linkage mapping in this study mapped Rpp6 from 5,953,237 to 5,998,461 bp (LOD score of 58.3) and the resistance from PI 567068A from 5,998,461 to 6,160,481 bp (LOD score of 4.4) (Wm82. a1 genome sequence). QTL peaks were 139,033 bp apart from one another as determined by the most significant SNPs in QTL mapping. The results of haplotype analysis demonstrated that PI 567102B and PI 567068A share the same haplotype in the resistance locus containing both Rpp alleles, which was designated as the Rpp6/Rpp[PI567068A] haplotype. The Rpp6/Rpp[PI567068A] haplotype identified in this study can be used as a tool to rapidly screen other genotypes that possess a Rpp gene(s) and detect resistance at the Rpp6 locus in diverse germplasm. C1 [King, Zachary R.; Harris, Donna K.; Li, Zenglu; Boerma, H. Roger] Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. [Pedley, Kerry F.] USDA ARS, Foreign Disease Weed Sci Res Unit, Frederick, MD 21702 USA. [Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Buck, James W.] Univ Georgia, Dept Plant Pathol, Griffin, GA 30223 USA. [Wang, Dechun; Wen, Zixiang] Michigan State Univ, Soybean Genet Lab, E Lansing, MI 48824 USA. RP Li, ZL (reprint author), Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. EM zli@uga.edu FU United Soybean Board [USB 1420-532-5635]; United Soybean Board FX We would like to acknowledge David Spradlin, Brian Vermeer, Dale Wood, Gina Bishop, Earl Baxter, Tatyana Nienow, and Colleen Wu at the University of Georgia for providing technical support in greenhouse assays in Griffin Georgia or genotyping support in the Soybean Molecular Breeding Laboratory. We would also like to acknowledge Amy Ruck for providing technical assistance in phenotyping plant introductions with a panel of Phakopsora pachyrhizi isolates described here at the USDA-ARS Foreign Disease-Weed Science Research Unit at Ft. Detrick, Maryland. This work was funded by the United Soybean Board (USB 1420-532-5635) and the United Soybean Board Fellowship to Zachary King. NR 45 TC 1 Z9 1 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2016 VL 129 IS 3 BP 517 EP 534 DI 10.1007/s00122-015-2645-3 PG 18 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DH6LX UT WOS:000372903200006 PM 26704418 ER PT J AU Khrustaleva, L Jiang, JM Havey, MJ AF Khrustaleva, Ludmila Jiang, Jiming Havey, Michael J. TI High-resolution tyramide-FISH mapping of markers tightly linked to the male-fertility restoration (Ms) locus of onion SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID IN-SITU HYBRIDIZATION; ALLIUM-CEPA L.; OPEN-POLLINATED POPULATIONS; INTEGRATED CYTOGENETIC MAP; ULTRA-SENSITIVE FISH; SINGLE-COPY GENE; CHROMOSOMAL REARRANGEMENTS; LINKAGE DISEQUILIBRIUM; CYTOPLASMIC GENOTYPES; PACHYTENE CHROMOSOMES AB Key message Tyramide FISH was used to locate relatively small genomic amplicons from molecular markers linked to Ms locus onto onion chromosome 2 near the centromere, a region of relatively low recombination. Fluorescence in situ hybridization (FISH) has not been readily exploited for physical mapping of molecular markers in plants due to the technical challenge of visualizing small single-copy probes. Signal amplification using tyramide (tyr) FISH can increase sensitivity up to 100-fold. We used tyr-FISH to physically locate molecular markers tightly linked to the nuclear male-fertility (Ms) restoration locus of onion onto mitotic metaphase, pachytene, and super-stretched pachytene chromosomes. Relatively short genomic amplicons (846-2251 bp) and a cDNA clone (666 bp) were visualized in 9-42 % of observed cells. The markers were assigned to proximal locations close to the centromere on the long arm of chromosome 2, a region of lower recombination, revealing that tightly linked markers may be physically distant from Ms. This result explains why several labs have identified molecular markers tightly linked to the Ms locus after screening relatively few DNA clones or primers and segregating progenies. Although these markers are still useful for marker-aided selection, our results indicate that map-based cloning of Ms will likely be difficult due to reduced recombination near this gene. C1 [Khrustaleva, Ludmila] Russian State Agr Univ, Ctr Mol Biotechnol, Dept Genet & Biotechnol, Timiryazev Agr Acad, 49 Timiryazevskaya Str, Moscow 127550, Russia. [Jiang, Jiming] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Havey, Michael J.] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA. [Havey, Michael J.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. RP Khrustaleva, L (reprint author), Russian State Agr Univ, Ctr Mol Biotechnol, Dept Genet & Biotechnol, Timiryazev Agr Acad, 49 Timiryazevskaya Str, Moscow 127550, Russia. EM ludmila.khrustaleva19@gmail.com FU Fulbright-Hayes Program [ID 68130146] FX We gratefully acknowledge the support of a research fellowship to LK (IIE Grantee ID 68130146) from the Fulbright-Hayes Program. NR 71 TC 1 Z9 1 U1 5 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAR PY 2016 VL 129 IS 3 BP 535 EP 545 DI 10.1007/s00122-015-2646-2 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DH6LX UT WOS:000372903200007 PM 26704420 ER PT J AU Maynard, RJ Aall, NC Saenz, D Hamilton, PS Kwiatkowski, MA AF Maynard, Ross J. Aall, Nathalie C. Saenz, Daniel Hamilton, Paul S. Kwiatkowski, Matthew A. TI Road-edge Effects on Herpetofauna in a Lowland Amazonian Rainforest SO TROPICAL CONSERVATION SCIENCE LA English DT Article DE edge effects; herpetofauna; roads; Neotropical; amphibians; reptiles ID AMPHIBIAN DIVERSITY; EASTERN ECUADOR; HABITAT; FROGS; FRAGMENTATION; ABUNDANCE; RESERVE; LIZARDS; ASSEMBLAGES; MECHANISMS AB The impact of roads on the flora and fauna of Neotropical rainforest is perhaps the single biggest driver of habitat modification and population declines in these ecosystems. We investigated the road-edge effect of a low-use dirt road on amphibian and reptile abundance, diversity, and composition within adjacent lowland Amazonian rainforest at San Jose de Payamino, Ecuador. The road has been closed to vehicle traffic since its construction in 2010. Thus, effects from vehicle mortality, vehicle-related pollution, and road noise were not confounding factors. Herpetofauna were surveyed using both visual encounter surveys and drift fences with pitfall and funnel traps at varying distances from the road. Structural and microclimate features of the forest were measured at each sampling distance. Several habitat variables were found to differ at intermediate and interior sampling distances from the road compared to forest edge conditions, suggesting the road-edge effect began to attenuate by the intermediate sampling distance. However, the edge effect on amphibians and reptiles appeared to extend 100 m from the road edge, as abundance and diversity were significantly greater at the interior forest compared to the forest edge. Additionally, assemblage composition as well as the hierarchical position of species shifted between sampling distances. Habitat predictor models indicate that amphibian abundance was best predicted by vine abundance, while both vine and mature tree abundance were the best predictors for species richness and diversity. Overall, and contrary to what might otherwise be expected, our results demonstrate that small, little-used road disturbances can nonetheless have profound impacts on wildlife. C1 [Maynard, Ross J.; Aall, Nathalie C.; Hamilton, Paul S.] Biodivers Grp, Tucson, AZ USA. [Maynard, Ross J.; Kwiatkowski, Matthew A.] Stephen F Austin State Univ, Dept Biol, Nacogdoches, TX 75962 USA. [Saenz, Daniel] US Forest Serv, Southern Res Stn, USDA, Nacogdoches, TX USA. RP Maynard, RJ (reprint author), Biodivers Grp, Tucson, AZ USA.; Maynard, RJ (reprint author), Stephen F Austin State Univ, Dept Biol, Nacogdoches, TX 75962 USA. EM ross@biodiversitygroup.org FU Biodiversity Group FX The authors are grateful to The Biodiversity Group for financial and logistic support of the project. We thank the community of San Jose de Payamino for giving permission to conduct this study in their territory as well as providing logistical support. Our gratitude goes to Santiago Ron for his assistance with permit acquisition to work in Ecuador. Paul Bamford was instrumental in acquiring information about the history of road construction in the community, and Javier Patino provided additional details pertaining to the study area. We thank Robert Anthony Villa for the Spanish translation of the abstract. Helpful revisions to the manuscript were provided by Chris Schalk. RJM and NCA are greatly appreciative for assistance in the field by various community members as well as many staff and volunteers at the Timburi Cocha Research Station and The Biodiversity Group, notably: Ryan L. Lynch, Lucas Huggins, Xaali O'Reilly Berkeley, Richard Preziosi, William Vigay, Rachel Hawthorn, Oliver Walker, James Stanton, Scott Trageser, Ash Wisco, and Javier Patino. NR 62 TC 0 Z9 0 U1 27 U2 38 PU TROPICAL CONSERVATION SCIENCE PI MENLO PARK PA PO BOX 0291, MENLO PARK, CA 94026-0291 USA SN 1940-0829 J9 TROP CONSERV SCI JI Trop. Conserv. Sci. PD MAR PY 2016 VL 9 IS 1 BP 264 EP 290 PG 27 WC Biodiversity Conservation SC Biodiversity & Conservation GA DI1ZQ UT WOS:000373295900014 ER PT J AU Larson, KL Nelson, KC Samples, SR Hall, SJ Bettez, N Cavender-Bares, J Groffman, PM Grove, M Heffernan, JB Hobbie, SE Learned, J Morse, JL Neill, C Ogden, LA O'Neil-Dunne, J Pataki, DE Polsky, C Chowdhury, RR Steele, M Trammell, TLE AF Larson, K. L. Nelson, K. C. Samples, S. R. Hall, S. J. Bettez, N. Cavender-Bares, J. Groffman, P. M. Grove, M. Heffernan, J. B. Hobbie, S. E. Learned, J. Morse, J. L. Neill, C. Ogden, L. A. O'Neil-Dunne, J. Pataki, D. E. Polsky, C. Chowdhury, R. Roy Steele, M. Trammell, T. L. E. TI Ecosystem services in managing residential landscapes: priorities, value dimensions, and cross-regional patterns SO URBAN ECOSYSTEMS LA English DT Article DE Lawns; Residential landscapes; Land management; Human values; Ecosystem services; Urban sustainability ID ENVIRONMENTAL ATTITUDES; URBAN; MANAGEMENT; CARE; HOMOGENIZATION; CLASSIFICATION; TRADEOFFS; PLACE; COVER; SENSE AB Although ecosystem services have been intensively examined in certain domains (e.g., forests and wetlands), little research has assessed ecosystem services for the most dominant landscape type in urban ecosystems-namely, residential yards. In this paper, we report findings of a cross-site survey of homeowners in six U.S. cities to 1) examine how residents subjectively value various ecosystem services, 2) explore distinctive dimensions of those values, and 3) test the urban homogenization hypothesis. This hypothesis posits that urbanization leads to similarities in the social-ecological dynamics across cities in diverse biomes. By extension, the thesis suggests that residents' ecosystem service priorities for residential landscapes will be similar regardless of whether residents live in the humid East or the arid West, or the warm South or the cold North. Results underscored that cultural services were of utmost importance, particularly anthropocentric values including aesthetics, low-maintenance, and personal enjoyment. Using factor analyses, distinctive dimensions of residents' values were found to partially align with the Millennium Ecosystem Assessment's categories (provisioning, regulating, supporting, and cultural). Finally, residents' ecosystem service priorities exhibited significant homogenization across regions. In particular, the traditional lawn aesthetic (neat, green, weed-free yards) was similarly important across residents of diverse U.S. cities. Only a few exceptions were found across different environmental and social contexts; for example, cooling effects were more important in the warm South, where residents also valued aesthetics more than those in the North, where low-maintenance yards were a greater priority. C1 [Larson, K. L.] Arizona State Univ, Sch Geog Sci & Urban Planning, Sch Sustainabil, Mail Code 5302, Tempe, AZ 85287 USA. [Nelson, K. C.] Univ Minnesota, Dept Forest Resources, 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. [Nelson, K. C.] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. [Samples, S. R.] Arizona State Univ, Herberger Inst Design & Arts, Tempe, AZ 85287 USA. [Hall, S. J.; Learned, J.] Arizona State Univ, Sch Life Sci, 427 E Tyler Mall, Tempe, AZ 85287 USA. [Bettez, N.; Groffman, P. M.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Cavender-Bares, J.; Grove, M.; Hobbie, S. E.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Cavender-Bares, J.; Grove, M.; Hobbie, S. E.] US Forest Serv, No Res Stn, Suite 350,5523 Res Pk Dr, Baltimore, MD 21228 USA. [Heffernan, J. B.] Duke Univ, Nicholas Sch Environm, Box 90328, Durham, NC 27708 USA. [Morse, J. L.] Portland State Univ, Dept Environm Sci & Management, POB 751, Portland, OR 97207 USA. [Neill, C.] Marine Biol Lab, Ctr Ecosyst, 7 MBL St, Woods Hole, MA 02543 USA. [Ogden, L. A.] Dartmouth Coll, Dept Anthropol, HB 6047 Silsby Hall, Hanover, NH 03755 USA. [O'Neil-Dunne, J.] Rubenstein Sch Environm & Nat Resources, Spatial Anal Lab, 205 George D Aiken Ctr, Burlington, VT 05405 USA. [Pataki, D. E.; Trammell, T. L. E.] Univ Delaware, Dept Plant & Soil Sci, 531 S Coll Ave,152 Townsend Hall, Newark, DE 19716 USA. [Polsky, C.] Florida Atlantic Univ, Florida Ctr Environm Studies, FL 3200 Coll Ave,Bldg DW Davie, Boca Raton, FL 33314 USA. [Chowdhury, R. Roy] Indiana Univ, Dept Geog, Student Bldg 120,701 E Kirkwood Ave, Bloomington, IN 47405 USA. [Steele, M.] Virginia Tech, Dept Crop & Soil Environm Sci, Smyth Hall,185 Ag Quad Ln 330, Blacksburg, VA 24061 USA. RP Larson, KL (reprint author), Arizona State Univ, Sch Geog Sci & Urban Planning, Sch Sustainabil, Mail Code 5302, Tempe, AZ 85287 USA. EM kelli.larson@asu.edu RI Heffernan, James/D-1261-2010; OI Heffernan, James/0000-0001-7641-9949; Hobbie, Sarah/0000-0001-5159-031X; Morse, Jennifer/0000-0001-8872-4940 FU MacroSystems Biology Program in the Emerging Frontiers Division of the Biological Sciences Directorate at the National Science Foundation (NSF) [EF-1065548, 1065737, 1065740, 1065741, 1065772, 1065785, 1065831, 121238320]; NSF [DEB-0423476, SES-0951366]; Phoenix [BCS-1026865]; Plum Island (Boston) [OCE-1058747]; Cedar Creek (Minneapolis-St Paul) [DEB-0620652]; Florida Coastal Everglades (Miami) [DBI-0620409] FX This work was supported by the MacroSystems Biology Program in the Emerging Frontiers Division of the Biological Sciences Directorate at the National Science Foundation (NSF) under grants EF-1065548, 1065737, 1065740, 1065741, 1065772, 1065785, 1065831, 121238320. The work arose from research funded by grants from the NSF Long-Term Ecological Research Program supporting work in Baltimore (DEB-0423476), Phoenix (BCS-1026865), Plum Island (Boston) (OCE-1058747), Cedar Creek (Minneapolis-St Paul) (DEB-0620652), and Florida Coastal Everglades (Miami) (DBI-0620409). This research was also supported by the NSF-funded Decision Center for a Desert City II: Urban Climate Adaptation (SES-0951366). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of NSF. NR 53 TC 3 Z9 3 U1 17 U2 35 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD MAR PY 2016 VL 19 IS 1 BP 95 EP 113 DI 10.1007/s11252-015-0477-1 PG 19 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA DH1LR UT WOS:000372546000005 ER PT J AU Yesilonis, ID Pouyat, RV Russell-Anelli, J Powell, E AF Yesilonis, Ian D. Pouyat, R. V. Russell-Anelli, J. Powell, E. TI The effects of landscape cover on surface soils in a low density residential neighborhood in Baltimore, Maryland SO URBAN ECOSYSTEMS LA English DT Article DE Forest; Urban; Soil; Lawn; Ecotope; Nutrients ID LAND-USE; URBAN SOILS; ECOSYSTEMS; CARBON; VARIABILITY; GRASSES; CITY AB Previous studies at the scale of a city have shown that surface soil nutrients, pH, and soil organic matter (SOM) can vary by land cover, land use, and management. This study was conducted in Baltimore County, Maryland, to quantify the differences in characteristics of soil in a residential neighborhood and adjacent forest patch sampling at a fine scale. The first objective was to compare soil characteristics in a residential neighborhood among ecotope types of forest, lawn, and planting beds that were underlain by the same parent material and thus only differed in plant cover. Another objective was to examine differences in soil properties of lawn soils that differed in age by 10 years. The final objective was to quantify the variation of these residential and forest soils. Composite soil samples from the surface to a depth of 5 cm were taken from planting beds and lawns from 50 residences and an adjacent forest patch. Results showed that the forest soil had 30 % more SOM and was more acidic than lawn soil. Conversely, Mg, P, K, and Ca were 47 to 67 % lower in forest compared to lawn soils even though both soils developed from similar parent materials. For the residential lawns, the older development had significantly higher concentration of soil P. There was also a difference between front and back lawns where front lawns had 26 and 10 % higher concentrations of Ca and Mg, respectively, and a higher pH than the back lawns. Finally, the variation of soil characteristics of all areas sampled, from lowest to highest was pH < SOM < K < Mg < Ca < P. Results of this study suggest that anthropogenic factors appear to overwhelm natural soil forming factors in suburban residential areas in the Baltimore metropolitan area and these differences appear to increase with time. C1 [Yesilonis, Ian D.] US Forest Serv, USDA, 5523 Res Pk,Suite 350, Baltimore, MD 21228 USA. [Pouyat, R. V.] US Forest Serv, USDA, Res & Dev, Washington, DC 20250 USA. [Russell-Anelli, J.] Cornell Univ, Dept Crop & Soil Sci, 624 Bradfield, Ithaca, NY 14853 USA. [Powell, E.] UMBC, Ctr Urban Environm Res & Educ, Technol Res Ctr 102, 1000 Hilltop Circle, Baltimore, MD 21250 USA. RP Yesilonis, ID (reprint author), US Forest Serv, USDA, 5523 Res Pk,Suite 350, Baltimore, MD 21228 USA. EM iyesilonis@fs.fed.us FU USDA Forest Service's Northern Research Station, Syracuse, NY [NRS-8]; Baltimore Ecosystem Study grant from the National Science Foundation [0423476]; University of Maryland Baltimore County, Center for Urban Environmental grants from the National Oceanic and Atmospheric Administration [NA06OAR4310243, NA07OAR4170518] FX Funding support was provided by the USDA Forest Service's Northern Research Station (NRS-8), Syracuse, NY; Baltimore Ecosystem Study grant from the National Science Foundation (Grant No. 0423476); University of Maryland Baltimore County, Center for Urban Environmental grants from the National Oceanic and Atmospheric Administration (NA06OAR4310243 and NA07OAR4170518). Thanks to Lauren Olszewski, Emily Neral, Ellen Henrikson, Chris Havran, Kim Mead, and Rosemary Williams who were responsible for the collection and processing of samples analyzed in this work. Thanks to John Stanovick, USDA Forest Service, for statistical review. NR 31 TC 0 Z9 0 U1 7 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD MAR PY 2016 VL 19 IS 1 BP 115 EP 129 DI 10.1007/s11252-015-0502-4 PG 15 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA DH1LR UT WOS:000372546000006 ER PT J AU Fares, A Safeeq, M Awal, R Fares, S Dogan, A AF Fares, Ali Safeeq, Mohammad Awal, Ripendra Fares, Samira Dogan, Ahmet TI Temperature and Probe-to-Probe Variability Effects on the Performance of Capacitance Soil Moisture Sensors in an Oxisol SO VADOSE ZONE JOURNAL LA English DT Article ID TIME-DOMAIN REFLECTOMETRY; WATER-CONTENT; ELECTRICAL-CONDUCTIVITY; DIELECTRIC SENSOR; CALIBRATION; FREQUENCY; VALIDATION; MANAGEMENT AB Reliable and accurate monitoring of soil water content (theta) across the landscape is indispensable for many water resources applications. Capacitance-based in situ soil water content measuring devices are extensively used despite their sensitivity to soil properties besides water content, e.g., temperature and organic matter content. The main goals of this study were to: (i) examine the effects of temperature, hysteresis of the temperature response, and probe-to-probe variability on the performance of three (5TE, EC-5, and EC-TM) single capacitance sensors (SCS) in a Hawaiian Oxisol; and (ii) develop empirical calibration equations to correct for temperature and improve measurement accuracy. The SCS raw output and thermocouple temperature measurements were recorded at 1-min intervals during heating and cooling cycles between 1 and 45 degrees C. The three SCS and thermocouples were inserted in uniformly packed soils with theta varying from 0 to 0.55 m(3) m(-3). We used three probes for each SCS, and the entire experiment was replicated with two heating and cooling cycles. Temperature, hysteresis, and the probe-to-probe variability effects were highly significant (p < 0.05) for all three SCS. Estimated theta using soil-specific calibrations at 25 degrees C significantly increased with increasing temperature for all SCS. The 5TE sensor showed increasing temperature sensitivity with increasing water content. However, the EC-5 and EC-TM sensors exhibited a bidirectional response to temperature, with the highest sensitivity at similar to 0.10 m(3) m(-3) water content. An empirically derived temperature-dependent calibration equation substantially reduced the variability (>90% reduction in interquartile range) in measured water content due to changing soil temperature. Applying differing temperature corrections for heating and cooling did not improve the calibration any further. C1 [Fares, Ali; Awal, Ripendra; Fares, Samira] Prairie View A&M Univ, Prairie View, TX 77446 USA. [Safeeq, Mohammad] Univ Calif, Sierra Nevada Res Inst, Merced, CA 95343 USA. [Safeeq, Mohammad] US Forest Serv, Pacific Southwest Res Stn, Fresno, CA 93710 USA. [Dogan, Ahmet] Yildiz Tekn Univ, Dept Civil Engn, TR-34210 Istanbul, Turkey. RP Fares, A (reprint author), Prairie View A&M Univ, Prairie View, TX 77446 USA. EM Alfares@Pvamu.edu OI Awal, Ripendra/0000-0002-2453-2592 FU USDA-National Institute of Food and Agriculture (NIFA); Hatch Act Formula Grant at the University of Hawai'i; USDA-NIFA Evans-Allen funds at Prairie View AM University FX This project was partially supported by the USDA-National Institute of Food and Agriculture (NIFA), Hatch Act Formula Grant at the University of Hawai'i, and the USDA-NIFA Evans-Allen funds at Prairie View A&M University. NR 38 TC 1 Z9 1 U1 10 U2 23 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD MAR PY 2016 VL 15 IS 3 DI 10.2136/vzj2015.07.0098 PG 13 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DH5EO UT WOS:000372808500004 ER PT J AU Eschbaumer, M Stenfeldt, C Pacheco, JM Rekant, SI Arzt, J AF Eschbaumer, Michael Stenfeldt, Carolina Pacheco, Juan M. Rekant, Steven I. Arzt, Jonathan TI Effect of storage conditions on subpopulations of peripheral blood T lymphocytes isolated from naive cattle and cattle infected with foot-and-mouth disease virus SO VETERINARY CLINICAL PATHOLOGY LA English DT Article DE Cryopreservation; flow cytometry; immunophenotyping; peripheral blood mononuclear cells; storage ID MONONUCLEAR-CELLS; FLOW-CYTOMETRY; CRYOPRESERVATION; RESPONSES; FRESH; LIMITATIONS AB BackgroundImmunophenotyping of blood lymphocytes by flow cytometry is important in infectious disease research. In animal experiments and other longitudinal studies, the processing, prompt staining, and analysis of fresh samples is a logistical challenge and daily assay variation can confound data interpretation. ObjectiveThis study examined the feasibility of cryopreservation and deferred analysis of bovine peripheral blood T lymphocytes from normal or infected animals. MethodsPeripheral blood mononuclear cells were collected from 4 naive Holstein steers and 4 steers infected with foot-and-mouth-disease virus serotype Asia1. Identical aliquots were labeled and analyzed immediately, labeled for deferred analysis, or stored at -70 degrees C or over liquid nitrogen for up to 3 weeks before labeling. ResultsFreezing of unlabeled cells induced statistically significant changes in phenotypic recognition. In infected animals, the T-cell population increased by 28% and CD8(+) T cells by 32%, while total CD3(+) cells decreased by 16%, and CD4(+) T cells decreased by 12%. Subsequent storage of frozen cells for the duration of the study, however, had no significant effect. There was less than 20% relative change in subpopulation sizes, and storage at -70 degrees C or over liquid nitrogen was equivalent. ConclusionsDepending on the objectives and practical limitations of a study, deferred labeling of peripheral blood lymphocytes can be a viable option. Although frozen storage of lymphocytes can introduce some artifactual distortion of relative cell populations, frozen cells can be maintained in storage until all samples in a longitudinal study can be analyzed in batch under standardized conditions and without introducing further bias. C1 [Eschbaumer, Michael; Stenfeldt, Carolina; Pacheco, Juan M.; Rekant, Steven I.; Arzt, Jonathan] USDA ARS, Plum Isl Anim Dis Ctr, FADRU, POB 848, Greenport, NY 11944 USA. [Eschbaumer, Michael; Stenfeldt, Carolina; Rekant, Steven I.] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN USA. RP Arzt, J (reprint author), USDA ARS, Plum Isl Anim Dis Ctr, FADRU, POB 848, Greenport, NY 11944 USA. EM jonathan.arzt@ars.usda.gov OI Stenfeldt, Carolina/0000-0002-2074-3886; Pacheco, Juan/0000-0001-5477-0201; Arzt, Jonathan/0000-0002-7517-7893 FU CRIS project (USDA, Agricultural Research Service [ARS]) [1940-32000-057-00D]; Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQPM-13-X-00131]; PIADC Research Participation Program fellowships FX This work was funded in part by CRIS project 1940-32000-057-00D (USDA, Agricultural Research Service [ARS]), as well as through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQPM-13-X-00131. Michael Eschbaumer, Carolina Stenfeldt, and Steven Rekant are recipients of PIADC Research Participation Program fellowships, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the U.S. Department of Energy. The authors thank William Golde, Jared Patch, and Mary Kenney of USDA/ARS, PIADC, for their help with setting up the flow cytometry workflow, and the Animal Resource Branch at PIADC for assistance with animal procedures. The content is solely the responsibility of the authors and does not necessarily represent the official views of the USDA or any other federal entity. The sponsors had no involvement in the study design, in the collection, analysis, and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. NR 16 TC 2 Z9 2 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0275-6382 EI 1939-165X J9 VET CLIN PATH JI Vet. Clin. Pathol. PD MAR PY 2016 VL 45 IS 1 BP 110 EP 115 DI 10.1111/vcp.12327 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DH6TW UT WOS:000372925900013 PM 26802284 ER PT J AU Tait, RG AF Tait, Richard Gregory, Jr. TI Ultrasound Use for Body Composition and Carcass Quality Assessment in Cattle and Lambs SO VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE LA English DT Article DE Beef quality; Body composition; Genetic improvement; Lamb quality; Longissimus; Marbling; Ultrasound ID PREDICTING RETAIL PRODUCT; TRIMMABLE FAT; BEEF-CATTLE; ANGUS CATTLE; RUMP FAT; LIVE; PERCENTAGE; THICKNESS; TRAITS; WEIGHT AB Genetic evaluation for carcass quality traits has evolved over time, in large part because of introduction of new technology, such as ultrasound measures of body composition. Ultrasound-measured body composition traits emulate important carcass traits, are very informative-for selection purposes, are acquired before final selections of seed stock candidate animals are made, and have seen high adoption rates by beef seed stock producers. The Ultrasound Guidelines Council certifies technicians who collect and interpret ultrasound data for beef producers. Multiple traits important to beef quality and quantity are evaluated at the same time with one scan session. C1 [Tait, Richard Gregory, Jr.] ARS, Genet Breeding & Anim Hlth Res Unit, USDA, US Meat Anim Res Ctr, POB 166,844 Rd 313, Clay Ctr, NE 68933 USA. RP Tait, RG (reprint author), ARS, Genet Breeding & Anim Hlth Res Unit, USDA, US Meat Anim Res Ctr, POB 166,844 Rd 313, Clay Ctr, NE 68933 USA. EM jr.tait@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 4 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0749-0720 EI 1558-4240 J9 VET CLIN N AM-FOOD A JI Vet. Clin. N. Am.-Food Anim. Pract. PD MAR PY 2016 VL 32 IS 1 BP 207 EP + DI 10.1016/j.cvfa.2015.09.007 PG 13 WC Veterinary Sciences SC Veterinary Sciences GA DH4OM UT WOS:000372765300013 PM 26922120 ER PT J AU Dube, PJ Vanotti, MB Szogi, AA Garcia-Gonzalez, MC AF Dube, P. J. Vanotti, M. B. Szogi, A. A. Garcia-Gonzalez, M. C. TI Enhancing recovery of ammonia from swine manure anaerobic digester effluent using gas-permeable membrane technology SO WASTE MANAGEMENT LA English DT Article DE Anaerobic digestion; Waste management; Gas-permeable membranes; Swine manure; Ammonia recovery; Nutrient recovery ID WASTE-WATER; REMOVAL; AERATION; NITROGEN; SLURRY AB Gas-permeable membrane technology is useful to recover ammonia from manure. In this study, the technology was enhanced using aeration instead of alkali chemicals to increase pH and the ammonium (NH4+) recovery rate. Digested effluents from covered anaerobic swine lagoons containing 1465-2097 mg NH4+-N L-1 were treated using submerged membranes (0.13 cm(2) cm(-3)), low-rate aeration (120 mL air L-manure(-1) min(-1)) and nitrification inhibitor (22 mg L-1) to prevent nitrification. The experiment included a control without aeration. The pH of the manure with aeration rose from 8.6 to 9.2 while the manure without aeration decreased from 8.6 to 8.1. With aeration, 97-99% of the NH4+ was removed in about 5 days of operation with 96-98% recovery efficiency. In contrast, without aeration it took 25 days to treat the NH4+. Therefore, the recovery of NH4+ was five times faster with the low-rate aeration treatment. This enhancement could reduce costs by 70%. Published by Elsevier Ltd. C1 [Dube, P. J.; Vanotti, M. B.; Szogi, A. A.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. [Garcia-Gonzalez, M. C.] Agr Technol Inst Castilla & Leon ITACyL, Valladolid, Spain. RP Dube, PJ (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM Patrick.dube@ars.usda.gov FU USDA-ARS [6082-13630-005-00D] FX This article is part of USDA-ARS Project 6082-13630-005-00D "Innovative Bioresource Management Technologies for Enhanced Environmental Quality and value Optimization." We acknowledge the field and laboratory assistance of William Brigman and Chris Brown, USDA-ARS, Florence, SC, and the field sampling assistance of Mark Rice, North Carolina State University, Raleigh, NC. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 33 TC 0 Z9 0 U1 3 U2 9 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0956-053X J9 WASTE MANAGE JI Waste Manage. PD MAR PY 2016 VL 49 BP 372 EP 377 DI 10.1016/j.wasman.2015.12.011 PG 6 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DH3GU UT WOS:000372676300040 PM 26739456 ER PT J AU Perry, RW Brandebura, SC Risch, TS AF Perry, Roger W. Brandebura, Stephen C. Risch, Thomas S. TI Selection of tree roosts by male Indiana bats during the autumn swarm in the Ozark Highlands, USA SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE Arkansas; autumn swarm; fall; forest management; hibernacula; Indiana bat; Ozarks; roost selection ID FALSE DISCOVERY RATE; MYOTIS-SODALIS; BROWN BATS; FORESTS; FIRES AB We identified 162 roosts for 36 male Indiana bats (Myotis sodalis) across 3 study areas in the Ozarks of northern Arkansas, USA, during the autumn swarm (late Aug to late Oct, 2005 and 2006). Bats utilized 14 tree species; snags of shortleaf pine (Pinus echinata) were the most utilized (30% of roosts) and pines were selected over hardwoods. Diameter of trees and snags used for roosting ranged from 7.8cm to 68.6cm diameter at breast height (dbh), but bats used trees 20cm dbh at a greater proportion than their availability. Roosts were located in a number of different forested cover classes, including shelterwood and group selection stands that had undergone partial harvesting. Roosts in 2 of 3 study areas showed no differences in proportional use of forest cover classes versus availability of those classes. However, in one study area, mature forests (50yr old) that had been burned once recently and stands burned multiple times over the past 10 years were used at a greater proportion than their availability, whereas mature forests that were not burned were used at a lower proportion than their availability. An examination of stand age data indicated that 98% of all roosts were located in stands 38 years old, suggesting that this is an important age threshold for roost selection in the Ozark Mountains. Bats in 2 study areas roosted at lower slopes in the higher elevation portions of the study areas, whereas no selection for topographic aspect were observed in all 3 study areas. Our data indicate that perceived habitat selection by a species may differ within the same geographic region and these differences could be due to factors such as differing selection among individuals, differences in juxtaposition of landscape components and cover types, and differing biological components such as the distribution of predators and predator densities. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Perry, Roger W.] US Forest Serv, USDA, Southern Res Stn, POB 1270, Hot Springs, AR 71902 USA. [Brandebura, Stephen C.; Risch, Thomas S.] Arkansas State Univ, POB 599, State Univ, AR 72467 USA. RP Perry, RW (reprint author), US Forest Serv, USDA, Southern Res Stn, POB 1270, Hot Springs, AR 71902 USA. EM rperry03@fs.fed.us FU U.S. Forest Service; Ozark National Forest; Arkansas Game and Fish Commission FX We appreciate the help of all those that worked or volunteered in the field, especially M. Cibarich, K. Drake, and B. Reynolds. We are also grateful to all the field and logistical support provided by Ozark-St. Francis National Forest personnel. Funding was provided by the U.S. Forest Service, Ozark National Forest, and the Arkansas Game and Fish Commission through the efforts of D.B. Sasse. We thank W. M. Ford, E. R. Britzke, 3 anonymous reviewers, and Associate Editor H. K. Ober for helpful comments on an earlier draft. The use of trade or firm names in this publication is for reader information and does not imply endorsement of any product or service by the U.S. Department of Agriculture. NR 49 TC 0 Z9 0 U1 4 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD MAR PY 2016 VL 40 IS 1 BP 78 EP 87 DI 10.1002/wsb.624 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DH9ON UT WOS:000373125600012 ER PT J AU Akresh, ME King, DI AF Akresh, Michael E. King, David I. TI Eastern whip-poor-will breeding ecology in relation to habitat management in a pitch pine-scrub oak barren SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE aerial insectivore; Antrostomus vociferous; Caprimulgiformes; fire; nest survival; nightjar; population limitation; shrubland; thinning ID NEST SURVIVAL; COMMON NIGHTHAWKS; UNITED-STATES; BIRDS; BEHAVIOR; SITE; CONSERVATION; TEMPERATURE; POPULATIONS; MOONLIGHT AB Numerous wildlife species are dependent on the creation and maintenance of early successional forests, yet little is known about the effects of habitat management on some threatened species. One such species is the eastern whip-poor-will (Antrostomus vociferous), a nocturnal bird of conservation concern. We examined the effects of heavy thinning, mowing, burning, and herbicide treatments on this species by conducting point counts and nest searches on a pitch pine-scrub oak (Pinus rigida-Quercus ilicifolia) barren in western Massachusetts, USA, between 2006 and 2013. Our point-count data showed that the abundance of calling birds was greater in managed shrublands such as scrub oak barrens and heavily thinned pitch pine stands, compared to closed-canopy pitch pine and deciduous forest. We found a high number of whip-poor-will nests (n=26) and roosts (n=59), which we located primarily within managed shrublands. We did not search for nests in closed-canopy forests, and we were unable to determine the extent of their use of the forest edge for nesting. Nevertheless, birds selected nest sites under residual deciduous trees within the early successional forests; therefore, canopy cover appears to be important for nest placement at the nest-patch spatial scale, but not necessarily at a broader scale. Nests were found in both dense and sparse understory vegetation; none were found in vegetation patches that were <2 years since treatment. Estimated nest survival was 63% through incubation (daily survival rate=0.977, n=21), consistent with other published studies of nightjars in the United States and Canada. Creating and maintaining open-canopy early successional forests in pitch pine-scrub oak barrens, with the retention of some residual deciduous trees, should increase the amount of habitat suitable for courtship, roosting, and nesting by eastern whip-poor-wills. (c) 2016 The Wildlife Society. C1 [Akresh, Michael E.] Univ Massachusetts, Dept Environm Conservat, 204 Holdsworth Hall, Amherst, MA 01003 USA. [King, David I.] Univ Massachusetts, US Forest Serv, No Res Stn, 201 Holdsworth Hall, Amherst, MA 01003 USA. RP Akresh, ME (reprint author), Univ Massachusetts, Dept Environm Conservat, 204 Holdsworth Hall, Amherst, MA 01003 USA. EM makresh@eco.umass.edu FU U.S. Forest Service, Northern Research Station; American Wildlife Conservation Foundation FX We thank all of the field technicians for their tireless work, including B. Kramer, B. Bailey, A. Bielaski, B. Byers, C. Chandler, J. Glagowski, T. Maikath, N. Papian, D. Rivera, S. Roberts, J. Ryan-Small, J. Smetzer, M. Smith, G. Turschak, M. VandenBoom, J. Wampler, D. Weidemann, and N. Young. The U.S. Forest Service, Northern Research Station provided the funding for the project. Additional funding came from the American Wildlife Conservation Foundation. Thanks to P. Hunt, J. Ralston, F. Thompson, III, A. Vitz, and 2 anonymous reviewers for comments on previous drafts of this article. NR 50 TC 1 Z9 1 U1 9 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD MAR PY 2016 VL 40 IS 1 BP 97 EP 105 DI 10.1002/wsb.621 PG 9 WC Biodiversity Conservation SC Biodiversity & Conservation GA DH9ON UT WOS:000373125600014 ER PT J AU Gerringer, MB Lima, SL DeVault, TL AF Gerringer, Michael B. Lima, Steven L. DeVault, Travis L. TI Evaluation of an avian radar system in a midwestern landscape SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE airport; airport wildlife management; avian radar; bird strike; human-wildlife conflict ID MIGRATORY CANADA GEESE; OFFSHORE WIND FARM; BIRD STRIKES; CIVIL AIRCRAFT; WILDLIFE; RISK; COLLISIONS; MOVEMENTS; AURA AB Bird strikes in aviation are an increasing threat to both aircraft and human safety. Management efforts have focused largely on the immediate airport environment. Avian radar systems could potentially be useful in assessing bird strike threats at greater distances from the airport, at higher altitudes, and at night, but few studies have been conducted to assess the capabilities of avian radar systems. Thus, our goal was to assess the detection and tracking abilities of a commercially available avian radar system in an airport environment in Indiana, USA, during October 2011-March 2012. Transits by free-flying birds allowed us to assess radar tracking performance as influenced by flock size, altitude, and distance from the radar unit. Most of the single large-bird targets (raptors) observed within 2 nautical miles (NM) of the radar were tracked 1 time, but such targets were generally tracked <30% of the time observed. Flocks of large birds such as geese (Branta canadensis) and cranes (Grus canadensis) were nearly always tracked 1 time, and were generally tracked approximately 40-80% of the time observed, even those several NMs away from the radar unit. Our results suggest that avian radar can be a useful tool for monitoring bird flock activity at airports, but less so for monitoring single large-bird targets such as thermalling raptors. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Gerringer, Michael B.; Lima, Steven L.] Indiana State Univ, Dept Biol, 600 Chestnut St, Terre Haute, IN 47809 USA. [DeVault, Travis L.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. [Gerringer, Michael B.] Western Ecosyst Technol Inc, 415 17th St, Cheyenne, WY 82001 USA. RP DeVault, TL (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM travis.l.devault@aphis.usda.gov FU U.S. Federal Aviation Administration (FAA) FX We thank many people who helped with this project. P. Schmidt laid the early groundwork for the radar evaluation. Our technicians J. Kesner and J. Bodwell operated the radar system with great attention to detail. W. Mitchell provided advice on our statistical analyses. T. West from DeTect, Inc. was instrumental in teaching us how to operate the Merlin System. J. Beasley and the other security personnel at the Terre Haute International Airport helped immensely by securing a location for the radar and allowing access to the airport on a daily basis. The U.S. Federal Aviation Administration (FAA) provided funding and support. Opinions expressed in this study do not necessarily reflect current FAA policy decisions governing the control of wildlife on or near airports. We thank three anonymous reviewers and R. E. Kissell, Jr. for helpful comments on the manuscript. NR 43 TC 2 Z9 2 U1 7 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD MAR PY 2016 VL 40 IS 1 BP 150 EP 159 DI 10.1002/wsb.614 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DH9ON UT WOS:000373125600020 ER PT J AU Ganey, JL AF Ganey, Joseph L. TI Recommendations for snag retention in southwestern mixed-conifer and ponderosa pine forests: History and current status SO WILDLIFE SOCIETY BULLETIN LA English DT Editorial Material DE cavities; cavity-nesting birds; management guidelines; snag density; snag diameter; snag height; snags; snag size; snag species; wildlife habitat ID CAVITY-NESTING BIRDS; NORTHERN ARIZONA; POPULATIONS; EXCAVATION; MANAGEMENT; DYNAMICS; DENSITY; ROOSTS AB Snags provide habitat for numerous species of wildlife. Several authors have provided recommendations for snag retention in southwestern mixed-conifer and ponderosa pine (Pinus ponderosa) forests. Most recommendations were presented in terms of minimum snag density and/or size. I summarized the history of recommendations for snag retention in these forest types, and used data from a current study of snag populations (conducted within Coconino and Kaibab National Forests, north-central AZ, USA, 1997 through 2012) to assess congruence between existing snag populations and various recommendations. Most recommendations were based on studies of cavity-nesting birds; therefore, this analysis emphasized characteristics of snags containing excavated nest cavities. Proportions of plots that met minimum management targets varied among recommendations, ranging from 34% to 100% in mixed-conifer forest and from 7% to 95% in ponderosa pine forest. Failure to meet density targets often was caused by a shortage of snags that met minimum size criteria rather than by a shortage of snags. Many snags containing excavated nest cavities did not meet the minimum size criteria in some recommendations. It may be possible to reduce those minimum size criteria while still providing substrates for cavity-nesting birds. Studies explicitly linking snag size and density to demography of cavity-nesting birds are badly needed, however, as are studies documenting ecologically sustainable snag densities. Until such data are available, managers should continue to emphasize snag recruitment and retention, with the focus on larger snags, and to ensure that snags are well-distributed, but not uniformly distributed, across the landscape. (c) 2016 The Wildlife Society. C1 [Ganey, Joseph L.] US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP Ganey, JL (reprint author), US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM jganey@fs.fed.us NR 38 TC 1 Z9 1 U1 4 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD MAR PY 2016 VL 40 IS 1 BP 192 EP 201 DI 10.1002/wsb.609 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DH9ON UT WOS:000373125600025 ER PT J AU Harnly, J AF Harnly, James TI Importance of Accurate Measurements in Nutrition Research: Dietary Flavonoids as a Case Study SO ADVANCES IN NUTRITION LA English DT Article DE dietary flavonoids; antioxidants; mass spectrometry; proanthocyanidins; secondary metabolites ID PERFORMANCE LIQUID-CHROMATOGRAPHY; HYDROXYCINNAMIC ACID-DERIVATIVES; ANTIOXIDANT CAPACITY; MASS-SPECTROMETRY; HPLC-DAD; ESI-MSN; QUANTIFICATION; VALIDATION; FRUIT; FOODS AB Accurate measurements of the secondary metabolites in natural products and plant foods are critical for establishing relations between diet and health. There are as many as 50,000 secondary metabolites that may influence human health. Their structural and chemical diversity presents a challenge to analytical chemistry. With respect to flavonoids, putative identification is accessible, but positive identification and quantification are limited by the lack of standards. Quantification has been tested with use of both nonspecific and specific methods. Nonspecific methods, which include antioxidant capacity methods, fail to provide information on the measured components, suffer from numerous interferences, are not equatable, and are unsuitable for health research. Specific methods, such as LC with diode array and mass spectrometric detection, require the use of internal standards and relative molar response factors. These methods are relatively expensive and require a high level of expertise and experimental verification; however, they represent the only suitable means of relating health outcomes to specific dietary components. C1 [Harnly, James] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. RP Harnly, J (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. FU ASN Nutritional Sciences Council; Dietary Bioactive Components Research Interest Section; Agricultural Research Service of the USDA; NIH Office of Dietary [IAAAOD12026-001-01002] FX This article is a review from the symposium "What's New in Natural Products Analysis? Cutting-Edge Methods and Available Resources for Nutrition Research" held 1 April 2015 at the ASN Scientific Sessions and Annual Meeting at Experimental Biology 2015 in Boston, MA. The symposium was sponsored by the ASN Nutritional Sciences Council and the Dietary Bioactive Components Research Interest Section.; Supported by the Agricultural Research Service of the USDA and in part by interagency agreement IAAAOD12026-001-01002 from the NIH Office of Dietary Supplements NR 41 TC 2 Z9 2 U1 5 U2 9 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAR PY 2016 VL 7 IS 2 BP 375 EP 382 DI 10.3945/an.115.010470 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DH3FG UT WOS:000372672300012 PM 26980821 ER PT J AU Webb, NP Galloza, MS Zobeck, TM Herrick, JE AF Webb, Nicholas P. Galloza, Magda S. Zobeck, Ted M. Herrick, Jeffrey E. TI Threshold wind velocity dynamics as a driver of aeolian sediment mass flux SO AEOLIAN RESEARCH LA English DT Article DE Wind erosion; Dust; Erodibility; Transport; Sediment flux; Supply limitation ID SALTATING PARTICLES; FRICTION VELOCITY; SAND TRANSPORT; CHIHUAHUAN DESERT; SHEAR VELOCITY; SOIL SURFACES; NEW-MEXICO; EROSION; MODEL; TUNNEL AB Horizontal (saltation) mass flux is a key driver of aeolian dust emission. Estimates of the horizontal mass flux underpin assessments of the global dust budget and influence our understanding of the dust cycle and its interactions. Current equations for predicting horizontal mass flux are based on limited field data and are constrained to representing transport-limited equilibrium saltation, driven by the wind momentum flux in excess of an entrainment threshold. This can result in large overestimation of the sediment mass flux. Here we compare measurements of the soil entrainment threshold, horizontal mass flux, and their temporal variability for five undisturbed dryland soils to explore the role of threshold in controlling the magnitude of mass flux. Average and median entrainment threshold showed relatively small variability among sites and relatively small variability between seasons, despite significant differences in soil surface conditions. Physical and biological soil crusts had little effect on the threshold value, and threshold appeared to play a minor role in determining the magnitude of sediment transport. Our results suggest that horizontal mass flux was controlled more by the supply limitation and abrasion efficiency of saltators present as loose erodible material or originating from neighboring soil sources. The omission of sediment supply and explicit representation of saltation bombardment from horizontal flux equations is inconsistent with the process representation in dust emission schemes and contributes to uncertainty in model predictions. This uncertainty can be reduced by developing greater process fidelity in models to predict horizontal mass flux under both supply- and transport-limited conditions. Published by Elsevier B.V. C1 [Webb, Nicholas P.; Galloza, Magda S.; Herrick, Jeffrey E.] NMSU, USDA ARS Jornada Expt Range, MSC JER 3, Box 30003, Las Cruces, NM 88003 USA. [Zobeck, Ted M.] USDA ARS Wind Eros & Water Conservat Res Unit, 3810 4th St, Lubbock, TX 79415 USA. RP Webb, NP (reprint author), NMSU, USDA ARS Jornada Expt Range, MSC JER 3, Box 30003, Las Cruces, NM 88003 USA. EM nwebb@nmsu.edu FU Department of Interior, Bureau of Land Management FX This research was supported by funding from the Department of Interior, Bureau of Land Management. We thank Lauren Svejcar for assistance with site establishment and soil and vegetation surveys, Brad Cooper for sample collection and servicing the field sites, Dean Holder for assistance with the soil analyses, and the anonymous reviews for constructive comments on the manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The USDA is an equal opportunity provider and employer. NR 81 TC 1 Z9 1 U1 10 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD MAR PY 2016 VL 20 BP 45 EP 58 DI 10.1016/j.aeolia.2015.11.006 PG 14 WC Geography, Physical SC Physical Geography GA DG9CX UT WOS:000372381500005 ER PT J AU Pi, H Sharratt, B Feng, G Lei, J Li, X Zheng, Z AF Pi, H. Sharratt, B. Feng, G. Lei, J. Li, X. Zheng, Z. TI Validation of SWEEP for creep, saltation, and suspension in a desert-oasis ecotone SO AEOLIAN RESEARCH LA English DT Article DE Creep; Saltation; Suspension; SWEEP; WEPS; Wind erosion ID EROSION PREDICTION SYSTEM; THRESHOLD FRICTION VELOCITY; WIND EROSION; COLUMBIA PLATEAU; WINDBLOWN DUST; AIR-QUALITY; AGRICULTURAL FIELDS; SEDIMENT TRANSPORT; SANDY BEACH; WEPS AB Wind erosion in the desert oasis ecotone can accelerate desertification, but little is known about the susceptibility of the ecotone to wind erosion in the Tarim Basin despite being a major source of windblown dust in China. The objective of this study was to test the performance of the Single-event Wind Erosion Evaluation Program (SWEEP) in simulating soil loss as creep, saltation, and suspension in a desert oasis ecotone. Creep, saltation, and suspension were measured and simulated in a desert oasis ecotone of the Tarim Basin during discrete periods of high winds in spring 2012 and 2013. The model appeared to adequately simulate total soil loss (ranged from 23 to 2272 g m(-2) across sample periods) according to the high index of agreement (d = 0.76). The adequate agreement of the SWEEP in simulating total soil loss was due to the good performance of the model (d = 0.71) in simulating creep plus saltation. The SWEEP model, however, inadequately simulated suspension based upon a low d (<= 0.43). The slope estimates of the regression between simulated and measured suspension and difference of mean suggested that the SWEEP underestimated suspension. The adequate simulation of creep plus saltation thus provides reasonable estimates of total soil loss using SWEEP in a desert oasis environment. Published by Elsevier B.V. C1 [Pi, H.; Feng, G.; Lei, J.; Li, X.; Zheng, Z.] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Xinjiang, Peoples R China. [Sharratt, B.] USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. [Feng, G.] USDA ARS, 810 Highway 12 East, Mississippi State, MS 39762 USA. RP Sharratt, B (reprint author), USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. EM Brenton.sharratt@ars.usda.gov FU National Natural Science Foundation of China [41171019]; One Hundred Talented Researchers Program of Chinese Academy of Sciences; Special Major Science and Technology Projects in Xinjiang Uygur Autonomous Region [201130106-1]; West Light Foundation of the Chinese Academy of Sciences [XBBS201104] FX The authors are grateful to Dr. Lawrence Hagen (retired) at the USDA-ARS Wind Erosion Research Unit in Manhattan, KS and Dr. Larry Wagner at the USDA-ARS Agricultural Systems Research Unit in Ft. Collins, CO, for their assistance with the application of SWEEP. This research was supported by National Natural Science Foundation of China (Grant No. 41171019), One Hundred Talented Researchers Program of Chinese Academy of Sciences, the Special Major Science and Technology Projects in Xinjiang Uygur Autonomous Region (201130106-1) and West Light Foundation of the Chinese Academy of Sciences (XBBS201104). NR 57 TC 1 Z9 1 U1 4 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD MAR PY 2016 VL 20 BP 157 EP 168 DI 10.1016/j.aeolia.2016.01.006 PG 12 WC Geography, Physical SC Physical Geography GA DG9CX UT WOS:000372381500015 ER PT J AU Locke, DH Grove, JM AF Locke, Dexter H. Grove, J. Morgan TI Doing the Hard Work Where it's Easiest? Examining the Relationships Between Urban Greening Programs and Social and Ecological Characteristics SO APPLIED SPATIAL ANALYSIS AND POLICY LA English DT Article DE Adoption; Urban sustainability; Tree canopy; Geodemographics Baltimore; MD; Washington; D.C ID SOCIOECONOMIC-STATUS; SPATIAL-DISTRIBUTION; PLANT DIVERSITY; GROUP IDENTITY; VEGETATION; LANDSCAPE; GARDENS; TREES; NEIGHBORHOODS; ECOSYSTEMS AB In this paper we examine the performance of formal programs associated with tree plantings in Washington, D.C. and Baltimore, MD to understand the relationships between the implementation of urban greening programs and the social and ecological characteristics of a city. Previous research has examined variations in patterns of existing and possible tree canopy cover relative to different social theories. Less attention has been paid to the processes of how the current patterns of tree canopy cover have developed. The goal of this paper is to address this gap by examining current programs to increase tree canopy. This paper utilizes public records, administrative data, a geodemographic market segmentation database, and high-resolution land cover data to assess where programs work, who participates in these programs, and whom the programs fail to reach. Recruiting households to plant trees can be hard work. In this paper, we find that programs might be most successful where it is easiest but have the lowest need. Free or reduced-cost programs for tree planting on private lands were most effective in the most affluent neighborhoods of Washington, D.C. and Baltimore, MD. These areas tended to also have the most existing tree canopy on both private residential lands and the public right of way. An outcome of this research is a framework for further testing which land management strategies are most effective, where, and with whom in order to improve the ability to plan and enhance urban sustainability and resilience through urban forestry. C1 [Locke, Dexter H.] Clark Univ, Grad Sch Geog, 950 Main St, Worcester, MA 01610 USA. [Grove, J. Morgan] US Forest Serv, USDA, No Res Stn, Baltimore Field Stn,UMBC, 5200 Westland Blvd,TRC 171, Baltimore, MD 21227 USA. RP Locke, DH (reprint author), Clark Univ, Grad Sch Geog, 950 Main St, Worcester, MA 01610 USA. EM dexter.locke@gmail.com FU National Science Foundation Baltimore Ecosystem Study Long Term Ecological Research site [DEB-0423476]; Washington D.C./Baltimore Urban Long Term Research-Exploratory [DEB0948947]; Libby Fund Enhancement Award; Clark University; Warnock Foundation/The Baltimore Social Innovation Journal; Edna Bailey Sussman Foundation; Jessica Sanders (Casey Trees), Paul Gobster FX Most of this work was completed while working for the USDA Forest Service Northern Research Station, Baltimore Field Station. Support for this research was provided by National Science Foundation Baltimore Ecosystem Study Long Term Ecological Research site (DEB-0423476), and Washington D.C./Baltimore Urban Long Term Research-Exploratory (DEB0948947) grants, as well as the Libby Fund Enhancement Award and the Marion I. Wright '46 Travel Grant from Clark University, the Warnock Foundation/The Baltimore Social Innovation Journal, and the Edna Bailey Sussman Foundation. We thank Jessica Sanders (Casey Trees), Paul Gobster, and Lindsay Campbell (USDA Forest Service, Northern Research Station) for their helpful comments that strengthened the paper. Gillian Baine (Saint Ann's School) provided useful feedback on earlier versions of Figs. 2 and 3. We thank Michael Potts and Jim Woodworth (Casey Trees), and Jen Kullgren, Charlie Murphy, and Erik Dihle (TreeBaltimore) for providing data. Our greatest thanks go to those who actually requested or planted a tree. Because of them their neighborhoods are better places to live. NR 68 TC 0 Z9 0 U1 3 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1874-463X EI 1874-4621 J9 APPL SPAT ANAL POLIC JI Appl. Spat. Anal. Policy PD MAR PY 2016 VL 9 IS 1 BP 77 EP 96 DI 10.1007/s12061-014-9131-1 PG 20 WC Geography SC Geography GA DG8IL UT WOS:000372327000005 ER PT J AU Gamble, GR Park, B Yoon, SC Lawrence, KC AF Gamble, Gary R. Park, Bosoon Yoon, Seung-Chul Lawrence, Kurt C. TI Effect of Sample Preparation on the Discrimination of Bacterial Isolates Cultured in Liquid Nutrient Media Using Laser-Induced Breakdown Spectroscopy (LIBS) SO APPLIED SPECTROSCOPY LA English DT Article DE Laser-induced breakdown spectroscopy (LIBS); Bacteria identification; Chemometrics; Culture purification; Bacteria cell wall ID BIOMEDICAL APPLICATIONS; CATION-EXCHANGE; CELL-WALLS; PH; IDENTIFICATION AB Laser-induced breakdown spectroscopy (LIBS) is used as the basis for discrimination between two genera of gram-negative bacteria and two genera of gram-positive bacteria representing pathogenic threats commonly found in poultry processing rinse waters. Because LIBS-based discrimination relies primarily upon the relative proportions of inorganic cell components including Na, K, Mg, and Ca, this study aims to determine the effects of trace mineral content and pH found in the water source used to isolate the bacteria upon the reliability of the resulting discriminant analysis. All four genera were cultured using tryptic soy agar (TSA) as the nutrient medium, and were grown under identical environmental conditions. The only variable introduced is the source water used to isolate the cultured bacteria. Cultures of each bacterium were produced using deionized (DI) water under two atmosphere conditions, reverse osmosis (RO) water, tap water, phosphate buffered saline (PBS) water, and TRIS buffered water. After 3 days of culture growth, the bacteria were centrifuged and washed three times in the same water source. Bacteria were then freeze dried, mixed with microcrystalline cellulose, and a pellet was made for LIBS analysis. Principal component analysis (PCA) was used to extract related variations in LIBS spectral data among the four bacteria genera and six water types used to isolate the bacteria, and Mahalanobis discriminant analysis (MDA) was used for classification. Results indicate not only that the four genera can be discriminated from each other in each water type, but that each genus can be discriminated by water type used for isolation. It is concluded that in order for LIBS to be a reliable and repeatable method for discrimination of bacteria grown in liquid nutrient media, care must be taken to insure that the water source used in purification of the culture be precisely controlled regarding pH, ionic strength, and proportionate amounts of mineral cations present. C1 [Gamble, Gary R.; Park, Bosoon; Yoon, Seung-Chul; Lawrence, Kurt C.] USDA ARS, US Natl Poultry Res Ctr, Dept Agr, Qual & Safety Assessment Res Unit, Athens, GA 30605 USA. RP Gamble, GR (reprint author), USDA ARS, US Natl Poultry Res Ctr, Dept Agr, Qual & Safety Assessment Res Unit, Athens, GA 30605 USA. EM Gary.Gamble@ars.usda.gov NR 20 TC 0 Z9 0 U1 7 U2 12 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0003-7028 EI 1943-3530 J9 APPL SPECTROSC JI Appl. Spectrosc. PD MAR PY 2016 VL 70 IS 3 BP 494 EP 504 DI 10.1177/0003702815626679 PG 11 WC Instruments & Instrumentation; Spectroscopy SC Instruments & Instrumentation; Spectroscopy GA DH1OH UT WOS:000372554000009 PM 26819442 ER PT J AU Cameron, EZ White, AM Gray, ME AF Cameron, Elissa Z. White, Angela M. Gray, Meeghan E. TI Solving the Productivity and Impact Puzzle: Do Men Outperform Women, or are Metrics Biased? SO BIOSCIENCE LA English DT Article DE women in science; gender bias; ecology; geographic variation ID H-INDEX; RESEARCH PERFORMANCE; GENDER-DIFFERENCES; SELF-CITATIONS; SCIENCE; ECOLOGY; MOTHERHOOD; SCIENTISTS; PIPELINE; NUMBERS AB The attrition of women from science with increasing career stage continues, suggesting that current strategies are unsuccessful. Research evaluation using unbiased metrics could be important for the retention of women, because other factors such as implicit bias are unlikely to quickly change. We compare the publishing patterns of men and women within the discipline of ecology and show sexual dimorphism in self citation leading to higher h-index scores for men despite lower citations per paper, which is exacerbated by more career absences by women. However, if self-citations and non-research active years are excluded, there are no gender differences in research performance. The pattern is consistent across disciplines and may contribute to current geographic disparities in research performance, rewarding confident behavior and traditional career paths rather than research impact. Importantly, these changes would not disadvantage anyone, because self-citation does not indicate broader impact, and researchers should only be judged on their research-active career. C1 [Cameron, Elissa Z.] Univ Tasmania, Hobart, Tas 7001, Australia. [White, Angela M.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA USA. [Gray, Meeghan E.] Truckee Meadows Community Coll, Reno, NV USA. RP Cameron, EZ (reprint author), Univ Tasmania, Hobart, Tas 7001, Australia. EM elissa.cameron@utas.edu.au RI Cameron, Elissa/B-8053-2014 OI Cameron, Elissa/0000-0002-9243-0547 NR 50 TC 0 Z9 0 U1 10 U2 18 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD MAR PY 2016 VL 66 IS 3 BP 245 EP 252 DI 10.1093/biosci/biv173 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DG4MF UT WOS:000372045600011 ER PT J AU Selling, GW Byars, JA Utt, KD AF Selling, Gordon W. Byars, Jeffrey A. Utt, Kelly D. TI Rheological Studies on the Reaction of Zein with Polyethylenemaleic Anhydride SO CEREAL CHEMISTRY LA English DT Article ID CROSS-LINKING AGENT; PROTEIN; TEMPERATURE; GELATION; KINETICS; GLYOXAL; FIBERS AB There continues to be interest in developing solvent-resistant articles from biobased renewable materials to successfully compete with petrochemical products. It was previously shown that reaction of zein with polyethylenemaleic anhydride (PEMA) provides articles that are solvent resistant. The gelation kinetics for the reaction of PEMA with zein was investigated rheologically to better understand this chemistry. The reaction of the nucleophilic groups on zein with the anhydrides on PEMA is the main cause for the gelation reaction. The gelation time was defined as being the point when the elastic modulus (G') and viscous modulus (G '') cross. In this work, the rate of reaction, in terms of time to gelation, was studied in N,N-dimethylformamide solution for which the amount of PEMA, the reaction temperature, and the overall reaction concentration were varied. Exponential relationships were found between the gelation time and % PEMA, temperature, and % solids, as well as between elastic modulus with either % PEMA or % solids. The concentration of PEMA had the largest impact on gelation time, for which going from 2.5% PEMA to 6% PEMA reduced the gelation time from 63,114 to 1,576 s. The temperature dependence of this gelation reaction was well described by an Arrhenius plot with an apparent activation energy of 50.5 kJ/mol. C1 [Selling, Gordon W.; Utt, Kelly D.] USDA ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Byars, Jeffrey A.] USDA ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Selling, GW (reprint author), USDA ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM gordon.selling@ars.usda.gov NR 27 TC 0 Z9 0 U1 2 U2 2 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2016 VL 93 IS 2 BP 145 EP 149 DI 10.1094/CCHEM-05-15-0099-R PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DH1YF UT WOS:000372580200006 ER PT J AU Chen, MH Bergman, CJ AF Chen, Ming-Hsuan Bergman, Christine J. TI Vitamin E Homologs and gamma-Oryzanol Levels in Rice (Oryza sativa L.) During Seed Development SO CEREAL CHEMISTRY LA English DT Article ID TOCOTRIENOL BIOSYNTHESIS; TOCOPHEROL BIOSYNTHESIS; MILLING FRACTIONS; ACCUMULATION; GRAINS; STEROLS; REVEALS; MICE AB Vitamin E homologs (tocopherols and tocotrienols) and gamma-oryzanol have gained significant attention because of their proposed health benefits and ability to increase vegetable oil stability. Changes in the levels of these phytochemicals were examined during seed development. Rapid accumulation of tocopherols, tocotrienols, and gamma-oryzanol occurred during early seed development. During the middle stage of seed development, the levels of tocopherols decreased sharply, and the levels of tocotrienols either stayed the same or decreased slightly, whereas gamma-oryzanol continued to accumulate until maturity. The levels of these compounds remained constant from maturity to 15 days postmaturity. In conclusion, rice grain for nutraceutical and functional food applications should be harvested during its immature stage to obtain the highest amount of tocopherols, whereas the amounts of the other phytochemicals that were studied can be optimized by harvesting grain at maturity. C1 [Chen, Ming-Hsuan] USDA, ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Bergman, Christine J.] Univ Nevada, Dept Food & Beverage, Las Vegas, NV 89154 USA. RP Chen, MH (reprint author), USDA, ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM ming.chen@ars.usda.gov NR 34 TC 0 Z9 0 U1 5 U2 6 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAR-APR PY 2016 VL 93 IS 2 BP 182 EP 188 DI 10.1094/CCHEM-07-15-0152-R PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DH1YF UT WOS:000372580200011 ER PT J AU Fang, Y Sun, G Caldwell, P McNulty, SG Noormets, A Domec, JC King, J Zhang, ZQ Zhang, XD Lin, GH Zhou, GS Xiao, JF Chen, JQ AF Fang, Yuan Sun, Ge Caldwell, Peter McNulty, Steven G. Noormets, Asko Domec, Jean-Christophe King, John Zhang, Zhiqiang Zhang, Xudong Lin, Guanghui Zhou, Guangsheng Xiao, Jingfeng Chen, Jiquan TI Monthly land cover-specific evapotranspiration models derived from global eddy flux measurements and remote sensing data SO ECOHYDROLOGY LA English DT Article DE eddy covariance flux; evapotranspiration; ecosystem modelling; ecohydrology; FLUXNET ID LEAF-AREA INDEX; MEAN ANNUAL EVAPOTRANSPIRATION; ENERGY-BALANCE CLOSURE; PINE PLANTATIONS; CARBON-DIOXIDE; CLIMATE-CHANGE; FOREST EVAPOTRANSPIRATION; COVARIANCE MEASUREMENTS; SOUTHEASTERN US; WATER-BALANCE AB Evapotranspiration (ET) is arguably the most uncertain ecohydrologic variable for quantifying watershed water budgets. Although numerous ET and hydrological models exist, accurately predicting the effects of global change on water use and availability remains challenging because of model deficiency and/or a lack of input parameters. The objective of this study was to create a new set of monthly ET models that can better quantify landscape-level ET with readily available meteorological and biophysical information. We integrated eddy covariance flux measurements from over 200 sites, multiple year remote sensing products from the Moderate Resolution Imaging Spectroradiometer (MODIS), and statistical modelling. Through examining the key biophysical controls on ET by land cover type (i.e. shrubland, cropland, deciduous forest, evergreen forest, mixed forest, grassland, and savannas), we created unique ET regression models for each land cover type using different combinations of biophysical independent factors. Leaf area index and net radiation explained most of the variability of observed ET for shrubland, cropland, grassland, savannas, and evergreen forest ecosystems. In contrast, potential ET (PET) as estimated by the temperature-based Hamon method was most useful for estimating monthly ET for deciduous and mixed forests. The more data-demanding PET method, FAO reference ET model, had similar power as the simpler Hamon PET method for estimating actual ET. We developed three sets of monthly ET models by land cover type for different practical applications with different data availability. Our models may be used to improve water balance estimates for large basins or regions with mixed land cover types. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Fang, Yuan; Noormets, Asko; Domec, Jean-Christophe; King, John] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Sun, Ge; McNulty, Steven G.] USDA Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, Raleigh, NC 27606 USA. [Caldwell, Peter] USDA Forest Serv, Coweeta Hydrol Lab, Southern Res Stn, Otto, NC USA. [Domec, Jean-Christophe] Univ Bordeaux, Bordeaux Sci Agro UMR INRA ISPA 1391, Gradignan, France. [Zhang, Zhiqiang] Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing, Peoples R China. [Zhang, Xudong] Chinese Acad Forestry, Inst Forest Res, Beijing, Peoples R China. [Lin, Guanghui] Tsinghua Univ, Ctr Earth Syst Sci, Beijing 100084, Peoples R China. [Zhou, Guangsheng] Chinese Acad Sci, Inst Bot, Beijing, Peoples R China. [Xiao, Jingfeng] Univ New Hampshire, Earth Syst Res Ctr, Durham, NH 03824 USA. [Chen, Jiquan] Michigan State Univ, CGCEO, E Lansing, MI 48824 USA. [Chen, Jiquan] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. RP Sun, G (reprint author), USDA Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, Raleigh, NC 27606 USA. EM gesun@fs.fed.us RI Chen, Jiquan/D-1955-2009; OI Noormets, Asko/0000-0003-2221-2111 FU USDA Forest Service Eastern Forest Environmental Threat Assessment Center; DOE-BER Terrestrial Ecosystem Sciences Program [11-DE-SC-0006700-ER65189]; U.S. Department of Energy, Biological and Environmental Research [DE-FG02-04ER63917, DE-FG02-04ER63911]; CarboEuropeIP; FAO-GTOS-TCO; iLEAPS; Max Planck Institute for Biogeochemistry; National Science Foundation; University of Tuscia; Universite Laval; Environment Canada; U.S. Department of Energy FX This study was supported by the USDA Forest Service Eastern Forest Environmental Threat Assessment Center and by the DOE-BER Terrestrial Ecosystem Sciences Program (11-DE-SC-0006700-ER65189). This work used eddy covariance data acquired by the FLUXNET community and in particular by the following networks: AmeriFlux [U.S. Department of Energy, Biological and Environmental Research, Terrestrial Carbon Program (DE-FG02-04ER63917 and DE-FG02-04ER63911)], AfriFlux, AsiaFlux, CarboAfrica, CarboEuropeIP, CarboItaly, CarboMont, ChinaFlux, Fluxnet-Canada (supported by CFCAS, NSERC, BIOCAP, Environment Canada, and NRCan), GreenGrass, KoFlux, LBA, NECC, OzFlux, TCOS-Siberia, and the United States China Carbon Consortium (USCCC). We acknowledge the financial support to the eddy covariance data harmonization provided by CarboEuropeIP, FAO-GTOS-TCO, iLEAPS, Max Planck Institute for Biogeochemistry, National Science Foundation, University of Tuscia, Universite Laval and Environment Canada, and U.S. Department of Energy, and the database development and technical support from Berkeley Water Center, Lawrence Berkeley National Laboratory, Microsoft Research eScience, Oak Ridge National Laboratory, University of California, Berkeley, and University of Virginia. This work also used MODIS land subset (Oak Ridge National Laboratory Distributed Active Archive Center (ORNL DAAC). 2011. MODIS subsetted land products, Collection 5). We thank the anonymous reviewers for their constructive comments on the manuscript. NR 92 TC 3 Z9 3 U1 9 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD MAR PY 2016 VL 9 IS 2 BP 248 EP 266 DI 10.1002/eco.1629 PG 19 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DG8CG UT WOS:000372309400006 ER PT J AU Bisbing, SM Cooper, DJ D'Amore, DV Marshall, KN AF Bisbing, Sarah M. Cooper, David J. D'Amore, David V. Marshall, Kristin N. TI Determinants of conifer distributions across peatland to forest gradients in the coastal temperate rainforest of southeast Alaska SO ECOHYDROLOGY LA English DT Article DE conifer distributions; hydrologic gradient; coastal temperate rainforest; southeast Alaska ID ENVIRONMENTAL GRADIENTS; SALT-MARSH; HERBACEOUS VEGETATION; TREE SEEDLINGS; SITKA SPRUCE; USA; PLANTS; NITROGEN; GROWTH; PHOTOSYNTHESIS AB Wetland determination relies on assumptions that site hydrologic and edaphic conditions limit plant species to certain environments. For example, using species' wetland indicator status for wetland determination assumes that tolerance of wetland conditions best explains distributional patterns. However, abiotic and biotic factors often interact to create complex plant responses across different environments. To evaluate these interactions, we used a hydrologic gradient in the coastal temperate rainforest of southeast Alaska to (1) quantify the primary determinants of conifer distributions, (2) identify thresholds in environmental factors limiting species' success and (3) assess current wetland indicator status of local conifers (Pinus contorta, Picea sitchensis, and Tsuga heterophylla). Data were collected using a hierarchical sampling scheme and analyzed within a Bayesian framework. Topography and hydrologic regime were the primary determinants of distributional patterns, but species were limited by specific microsite factors. Competitively dominant P.sitchensis occurred where hydrology, pH, and nitrogen were most favorable for establishment, while stress-tolerant P.contorta was competitively excluded from these sites. Tsugaheterophylla occurred across the gradient but took advantage of drier conditions, which promoted biomass accumulation. Tree distributions were limited by the interaction between abiotic and biotic factors rather than by abiotic tolerance alone. This knowledge of local and regional drivers of species' distributions and the relative importance of interacting abiotic and biotic drivers provide critical information for land management and regulation. Wetland delineation procedures can be improved through application of the regional empirical limits identified for plant species, as implemented and addressed in this study. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Bisbing, Sarah M.] Calif Polytech State Univ San Luis Obispo, Dept Nat Resources Management & Environm Sci, 1 Grand Ave, San Luis Obispo, CA 93407 USA. [Cooper, David J.] Colorado State Univ, Forest & Rangeland Stewardship, 1472 Campus Mail, Ft Collins, CO 80523 USA. [D'Amore, David V.] USDA Forest Serv, Pacific NW Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. [Marshall, Kristin N.] Univ Washington, Sch Aquat & Fishery Sci, Box 355020, Seattle, WA USA. RP Bisbing, SM (reprint author), Calif Polytech State Univ San Luis Obispo, Dept Nat Resources Management & Environm Sci, 1 Grand Ave, San Luis Obispo, CA 93407 USA. EM sbisbing@calpoly.edu FU US Army Corps of Engineers Cold Regions Research and Engineering Laboratory (CRREL); USDA Pacific Northwest Research Station FX Funding was provided by the US Army Corps of Engineers Cold Regions Research and Engineering Laboratory (CRREL) and the USDA Pacific Northwest Research Station. We especially thank Robert Lichvar of CRREL for his support of this work. The authors thank Dr Dan Binkley and Dr Amy Angert for their thoughtful comments on this manuscript and Toni DeSanto, Reid Dihle, and Wes Swaffar for assistance with fieldwork. The authors are also grateful for reviewer and editor comments that greatly improved this manuscript. NR 75 TC 1 Z9 1 U1 5 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD MAR PY 2016 VL 9 IS 2 BP 354 EP 367 DI 10.1002/eco.1640 PG 14 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DG8CG UT WOS:000372309400014 ER PT J AU Hanberry, BB He, HS Shifley, SR AF Hanberry, Brice B. He, Hong S. Shifley, Stephen R. TI Loss of aboveground forest biomass and landscape biomass variability in Missouri, US SO ECOLOGICAL COMPLEXITY LA English DT Article DE Carbon; Disturbance; Fire; Heterogeneity; Homogenous; Naturalness; Restoration ID UNITED-STATES; FIRE; TREE AB Disturbance regimes and forests have changed over time in the eastern United States. We examined effects of historical disturbance (circa 1813 to 1850) compared to current disturbance (circa 2004 to 2008) on aboveground, live tree biomass (for trees with diameters >= 13 cm) and landscape variation of biomass in forests of the Ozarks and Plains landscapes in Missouri, USA. We simulated 10,000 one hectare plots using random diameters generated from parameters of diameter distributions limited to diameters >= 13 cm and random densities generated from density estimates. Area-weighted mean biomass density (Mg/ha) for historical forests averaged 116 Mg/ha, ranging from 54 Mg/ha to 357 Mg/ha by small scale ecological subsections within Missouri landscapes. Area-weighted mean biomass density for current forests averaged 82 Mg/ha, ranging from 66 Mg/ha to 144 Mg/ha by ecological subsection for currently forested land. Biomass density of current forest was greater than historical biomass density for only 2 of 23 ecological subsections. Current carbon sequestration of 292 TgC on 7 million ha of forested land is less than half of the estimated historical total carbon sequestration of 693 TgC on 12 million ha. Cumulative tree cutting disturbances over time have produced forests that have less aboveground tree biomass and are uniform in biomass compared to estimates of historical biomass, which varied across Missouri landscapes. With continued relatively low rates of forest disturbance, current biomass per ha will likely increase to historical levels as the most competitive trees become larger in size and mean number of trees per ha decreases due to competition and self-thinning. Restoration of large diameter structure and forested extent of upland woodlands and floodplain forests could fulfill multiple conservation objectives, including carbon sequestration. (C) 2015 Elsevier B.V. All rights reserved. C1 [Hanberry, Brice B.; He, Hong S.] Univ Missouri, 203 Nat Resources Bldg, Columbia, MO 65211 USA. [Shifley, Stephen R.] Univ Missouri, USDA, US Forest Serv, No Res Stn, 202 Nat Resources Bldg, Columbia, MO 65211 USA. RP Hanberry, BB (reprint author), Univ Missouri, 203 Nat Resources Bldg, Columbia, MO 65211 USA. EM hanberryb@gmail.com FU USDA Forest Service Northern Research Station; University of Missouri-Columbia FX This project was funded by the USDA Forest Service Northern Research Station and the University of Missouri-Columbia. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the views of the United States Government. NR 29 TC 0 Z9 0 U1 5 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1476-945X EI 1476-9840 J9 ECOL COMPLEX JI Ecol. Complex. PD MAR PY 2016 VL 25 BP 11 EP 17 DI 10.1016/j.ecocom.2015.11.001 PG 7 WC Ecology SC Environmental Sciences & Ecology GA DH3MG UT WOS:000372690500002 ER PT J AU Li, C Bai, G Carver, BF Chao, S Wang, Z AF Li, C. Bai, G. Carver, B. F. Chao, S. Wang, Z. TI Mapping quantitative trait loci for plant adaptation and morphology traits in wheat using single nucleotide polymorphisms SO EUPHYTICA LA English DT Article DE Triticum aestivum; Adaptation and morphology traits; SNP; Epistasis; QTL x environments interaction ID DOUBLED HAPLOID POPULATION; TRITICUM-AESTIVUM L.; X ENVIRONMENT INTERACTIONS; SPIKE-RELATED TRAITS; WINTER-WHEAT; BREAD WHEAT; AGRONOMIC TRAITS; GRAIN-YIELD; LODGING RESISTANCE; GENETIC-CONTROL AB Wheat (Triticum aestivum L.) morphological and adaptation-related traits that are controlled by quantitative trait loci (QTLs) help define potential growing areas of a wheat cultivar. To dissect QTLs for heading date, lodging, shattering (SH), cold tolerance, plant height, spike length, spike compactness, awn length (AL) and chaff color (CC), a high-density genetic map with single nucleotide polymorphism and simple sequence repeat markers were developed using recombinant inbred lines (RILs) derived from 'Ning7840' x 'Clark'. The RILs were evaluated in eight Oklahoma environments from 2001 to 2004. A total of 31 QTLs with additive effects on different traits were mapped on most wheat chromosomes except for 1D, 3A, 3D, 4D, 6D, and 7B. Six chromosome regions showed either tightly linked QTLs or QTLs with pleiotropic effects for two to three traits. Five QTL pairs showed additive x additive effects (AA). Ten additive QTLs were involved in additive x environment (AE) effects, and one epistatic QTL was involved in AAE effects. Among nine traits evaluated only three (SH, AL, and CC) were controlled by single genes in this biparental population. Seven traits were conditioned by multiple QTLs. A total of 127 markers were tightly linked to the QTLs. The findings shed light on the inheritance of wheat morphological and adaptationrelated traits and provide DNA markers for manipulating these important traits to improve wheat production. C1 [Li, C.; Wang, Z.] Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Bai, G.] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Li, C.; Bai, G.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Carver, B. F.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Chao, S.] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Bai, G (reprint author), USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM gbai@ksu.edu FU National Research Initiative Competitive Grants CAP project from USDA National Institute of Food and Agriculture [2011-68002-30029]; Science and Technology Innovation Team Plan from Shaanxi province, China [2014KCT-25] FX This is contribution number 15-390-J from the Kansas Agricultural Experiment Station. This project is partly funded by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture; the Science and Technology Innovation Team Plan (2014KCT-25) from Shaanxi province, China. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 1 Z9 1 U1 13 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAR PY 2016 VL 208 IS 2 BP 299 EP 312 DI 10.1007/s10681-015-1594-x PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DG6DG UT WOS:000372171800009 ER PT J AU Shapiro-Ilan, D Raymond, B AF Shapiro-Ilan, David Raymond, Ben TI Limiting opportunities for cheating stabilizes virulence in insect parasitic nematodes SO EVOLUTIONARY APPLICATIONS LA English DT Article DE biological control; cooperation; evolution of virulence; Heterorhabditis floridensis; pest management; stability ID HETERORHABDITIS-BACTERIOPHORA; ENTOMOPATHOGENIC NEMATODES; STEINERNEMA-CARPOCAPSAE; BENEFICIAL TRAITS; BACTERIAL VIRULENCE; GENETICAL EVOLUTION; VISCOUS POPULATIONS; COOPERATION; DETERIORATION; PHOTORHABDUS AB Cooperative secretion of virulence factors by pathogens can lead to social conflict when cheating mutants exploit collective secretion, but do not contribute to it. If cheats outcompete cooperators within hosts, this can cause loss of virulence. Insect parasitic nematodes are important biocontrol tools that secrete a range of significant virulence factors. Critically, effective nematodes are hard to maintain without live passage, which can lead to virulence attenuation. Using experimental evolution, we tested whether social cheating might explain unstable virulence in the nematode Heterorhabditis floridensis by manipulating relatedness via multiplicity of infection (MOI), and the scale of competition. Passage at high MOI, which should reduce relatedness, led to loss of fitness: virulence and reproductive rate declined together and all eight independent lines suffered premature extinction. As theory predicts, relatedness treatments had more impact under stronger global competition. In contrast, low MOI passage led to more stable virulence and increased reproduction. Moreover, low MOI lineages showed a trade-off between virulence and reproduction, particularly for lines under stronger between-host competition. Overall, this study indicates that evolution of virulence theory is valuable for the culture of biocontrol agents: effective nematodes can be improved and maintained if passage methods mitigate possible social conflicts. C1 [Shapiro-Ilan, David] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Raymond, Ben] Royal Holloway Univ London, Egham TW20 0EX, Surrey, England. [Raymond, Ben] Univ London Imperial Coll Sci Technol & Med, Silwood Pk Campus, Ascot, Berks, England. RP Raymond, B (reprint author), Royal Holloway Univ London, Egham TW20 0EX, Surrey, England. EM b.raymond@imperial.ac.uk FU Natural Environment Research Council Research Fellowship; USDA-ARS FX We thank two anonymous reviewers for their helpful comments. We also thank B. Bennett, S. Byrd, L. Fall, K. Halat and A. King for technical assistance. This work was supported by a Natural Environment Research Council Research Fellowship award (to B.R.) and by USDA-ARS. This study is a contribution from the Imperial College Grand Challenges in Ecosystems and the Environment initiative. NR 61 TC 0 Z9 0 U1 2 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD MAR PY 2016 VL 9 IS 3 BP 462 EP 470 DI 10.1111/eva.12348 PG 9 WC Evolutionary Biology SC Evolutionary Biology GA DG9MD UT WOS:000372406200005 PM 26989437 ER PT J AU Isas, M Perez, MLD Salvatore, A Gastaminza, G Willink, E White, W AF Isas, Marcos Perez, Mara L. del P. Salvatore, Analia Gastaminza, Gerardo Willink, Eduardo White, William TI Impacts of crop residue on damage by sugarcane pests during the tillering phase in Argentina SO FLORIDA ENTOMOLOGIST LA English DT Article DE true armyworm; lesser cornstalk borer; green harvest; trash blanket; stubble AB One of the most important recent changes in sugarcane cultural practices in Tucuman, Argentina, is the adoption of the practice of green-cane harvesting, which involves harvesting the sugarcane crop in the absence of burning. The objective of this study was to assess the impact of the post-harvest crop residue (also sometimes known as "trash blanket") on Elasmopalpus lignosellus (Zeller) (Lepidoptera: Pyralidae) and Pseudaletia unipuncta Haworth (Lepidoptera: Noctuidae) population dynamics in sugarcane. The study was conducted in ratoon crops for 3 growing seasons (2011, 2012, and 2013) and at 3 locations in the state of Tucuman, Argentina. The treatments consisted of rows with the crop residue burned and the crop residue retained. Removal of the crop residue by burning, compared with retention, resulted in significantly greater crop damage by E. lignosellus in all locations and years. In contrast, damage by P. unipuncta was observed exclusively on plots where crop residue was retained. Comparing the pest status of 2 insects, E. lignosellus seems to be more consistently deleterious to sugarcane yield than P. unipuncta, and chemical control of this species is not very effective. Therefore, leaving the crop residue in place seems to be the most appropriate crop management approach, although it is important also to monitor P. unipuncta populations in order to implement control should it be necessary. C1 [Isas, Marcos; Perez, Mara L. del P.; Salvatore, Analia; Gastaminza, Gerardo; Willink, Eduardo] Consejo Nacl Invest Cient & Tecn, Inst Tecnol Agroind Noroeste Argentino ITANOA, EEAOC, RA-4101 San Miguel De Tucuman, Argentina. [White, William] ARS, USDA, Sugarcane Res Lab, 5883 USDA Rd, Houma, LA 70360 USA. RP Isas, M (reprint author), Consejo Nacl Invest Cient & Tecn, Inst Tecnol Agroind Noroeste Argentino ITANOA, EEAOC, RA-4101 San Miguel De Tucuman, Argentina. EM mgisas@eeaoc.org.ar FU Estacion Experimental Agroindustrial Obispo Colombres (EEAOC); Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) of Argentina FX We thank Estacion Experimental Agroindustrial Obispo Colombres (EEAOC) and Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) of Argentina for financial support. We also thank anonymous reviewers for thoughtful comments on the manuscript. NR 30 TC 0 Z9 0 U1 1 U2 3 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2016 VL 99 IS 1 BP 1 EP 5 PG 5 WC Entomology SC Entomology GA DH1MP UT WOS:000372548900001 ER PT J AU Wheeler, GS Silverson, N Dyer, K Mc Kay, F AF Wheeler, G. S. Silverson, N. Dyer, K. Mc Kay, F. TI Brazilian collections and laboratory biology of the thrips Pseudophilothrips ichini (Thysanoptera: Phlaeothripidae): a potential biological control agent of the invasive weed Brazilian peppertree (Sapindales: Anacardiaceae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE Anacardiaceae; biological control of weeds; invasive species; Schinus terebinthifolia ID SCHINUS-TEREBINTHIFOLIUS SAPINDALES; FLORIDA; USA AB Brazilian peppertree, Schinus terebinthifolia Raddi (Sapindales: Anacardiaceae), is one of the most invasive weeds in Florida and Hawaii. In the invaded range, this fast-growing weed from South America poses a threat to agriculture and cattle production and decreases the biodiveristy of natural areas. The thrips Pseudophilothrips ichini (Hood) (Thysanoptera: Phlaeothripidae) is being studied as a potential agent for the biological control of this weed. The laboratory life history and native range of P. ichini in Brazil were examined over 10 yr. The thrips life history includes 2 feeding larval stages that occur on the plant and 3 non-feeding pupal stages that occur in the soil. Development time, body length, and distinct features of each life stage are described. The larva-to-adult development required 20 d, and adults lived for an average of 50 d. Pseudophilothrips ichini had a wide latitudinal range in Brazil along the eastern coast from Bahia (11.4 degrees S) south to Santa Catarina State (27.1 degrees S). It was collected from sea level to 1,329 m elevation. Observations in Brazil indicated that this thrips occurs year round and may occasionally reach high densities (>20 thrips/leaf). Despite searches in its native range of related plants, the thrips was found only on Brazilian peppertree. Considering the short generation time, broad environmental tolerance, host specificity, and damage caused to the host if this thrips is released for biological control, it will contribute significantly to the management of Brazilian peppertree. C1 [Wheeler, G. S.; Dyer, K.] ARS, Invas Plant Res Lab, USDA, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. [Silverson, N.] SCA AmeriCorps, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. [Mc Kay, F.] Fuedei, Buenos Aires, DF, Argentina. RP Wheeler, GS (reprint author), ARS, Invas Plant Res Lab, USDA, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM greg.wheeler@ars.usda.gov FU Florida Fish and Wildlife Conservation Commission [08250, TA:088]; South Florida Water Management District [4600001427]; United States Department of Agriculture, Agricultural Research Service FX We thank Megan Chawner and Jessica Rendon (SCA/AmeriCorps, Ft. Lauderdale, Florida) for technical assistance and M. Vitorino (Univ. Blumenau, Brazil) for field assistance. Insect identifications were generously provided for P. ichini by L. A. Mound and for Thripastichus sp. by J. La Salle (CSIRO, Canberra, Australia). Thrips voucher specimens are deposited in the Florida Department of Agriculture and Consumer Services. Thrips identity was confirmed by DNA barcode analysis conducted by D. Williams, TCU (GenBank accessions GU942810-GU942817). Insect collections and exportations were authorized by Instituto Brasileiro do Meio Ambiente (Permits No 09BR003939/DF, 14BR004731/DF). The importation permit to the USA was issued by the United States Department of Agriculture, Animal and Plant Health Inspection Service to G. Wheeler (Permit No P526P-07-06609). This project was partially funded by the Florida Fish and Wildlife Conservation Commission (#08250, TA:088), the South Florida Water Management District (#4600001427), and the United States Department of Agriculture, Agricultural Research Service. NR 36 TC 3 Z9 3 U1 2 U2 6 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2016 VL 99 IS 1 BP 6 EP 11 PG 6 WC Entomology SC Entomology GA DH1MP UT WOS:000372548900002 ER PT J AU Tipping, PW Martin, MR Pratt, PD Wheeler, GS Gettys, LA AF Tipping, Philip W. Martin, Melissa R. Pratt, Paul D. Wheeler, Gregory S. Gettys, Lyn A. TI Response of two chemotypes of Melaleuca quinquenervia (Myrtales: Myrtaceae) saplings to colonization by specialist herbivores SO FLORIDA ENTOMOLOGIST LA English DT Article DE Oxyops vitiosa; Boreioglycaspis melaleuca; E-nerolidol; viridiflorol ID BIOLOGICAL-CONTROL AGENT; OXYOPS-VITIOSA COLEOPTERA; BOREIOGLYCASPIS-MELALEUCAE; INVASIVE TREE; FIELD COLONIZATION; WOODY-PLANTS; GROWTH; CURCULIONIDAE; REPRODUCTION; METAANALYSIS AB Two chemotypes of Melaleuca quinquenervia (Cav.) S. T. Blake (Myrtales: Myrtaceae) saplings were planted in a common garden under 2 water treatments and exposed to either restricted or unrestricted herbivory from 2 insect herbivores. Chemotypes consisted of either a predominately E-nerolidol terpenoid complex or one that consisted primarily of viridiflorol; both types had influenced the performance or preference of both insect herbivores in laboratory studies. The densities of the 2 specialist herbivores Boreioglycaspis melaleucae Moore (Hemiptera: Psyllidae) and Oxyops vitiosa Pascoe (Coleoptera: Curculionidae) were monitored regularly as they colonized the plantings, as were plant responses like leaf abscission and tree height. Neither the chemotype nor the water treatments influenced the densities of B. melaleucae and O. vitiosa. Trees subjected to unrestricted herbivory shed more leaf biomass than those protected by insecticides from herbivory. There was no relationship between the amount of biomass shed via abscission and the density of either herbivore despite a significant correlation with a damage rating developed for O. vitiosa. There was a chemotype response to herbivory whereby more leaf biomass was shed by the E-nerolidol chemotype than by the viridiflorol chemotype when subjected to unrestricted herbivory. Tree height was influenced by herbivory but not chemotype or water, although there were separate 2-way interactions between all factors. Thus, despite equal herbivore pressure, the response of young M. quinquenervia trees to abiotic and biotic forces diverged at the plant variant level. C1 [Tipping, Philip W.; Wheeler, Gregory S.] ARS, USDA, Invas Plant Res Lab, Davie, FL 33314 USA. [Martin, Melissa R.] US Fish & Wildlife, ARM Loxahatchee Natl Wildlife Refuge, Delray Beach, FL 33473 USA. [Pratt, Paul D.] ARS, USDA, Exot & Invas Weeds Res, Albany, CA 94710 USA. [Gettys, Lyn A.] Univ Florida, Ft Lauderdale Res & Educ Ctr, Davie, FL 33314 USA. RP Tipping, PW (reprint author), ARS, USDA, Invas Plant Res Lab, Davie, FL 33314 USA. EM philip.tipping@ars.usda.gov NR 32 TC 0 Z9 0 U1 2 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2016 VL 99 IS 1 BP 77 EP 81 PG 5 WC Entomology SC Entomology GA DH1MP UT WOS:000372548900013 ER PT J AU Shoemaker, D AF Shoemaker, DeWayne TI Development of genetic markers distinguishing two invasive fire ant species (Hymenoptera: Formicidae) and their hybrids SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Solenopsis invicta; Solenopsis richteri; SNP ID ADAPTIVE TRAIT INTROGRESSION; HYBRIDIZATION; SUNFLOWERS; ZONE AB Three single nucleotide polymorphism (SNP) markers were developed that were completely diagnostic in distinguishing the 2 fire ant species Solenopsis invicta Buren and Solenopsis richteri Forel (Hymenoptera: Formicidae). Although a 4th marker was not fully diagnostic, it was still useful given that one of the variants was confined to S. richFteri. Joint use of these markers was shown to consistently distinguish hybrids from the 2 parental species. The development of these SNP markers distinguishing the 2 introduced fire ant species and their hybrids represents a significant advance over existing methods because this method is robust, easily transferable, and cost effective. C1 [Shoemaker, DeWayne] ARS, USDA, Gainesville, FL 32608 USA. RP Shoemaker, D (reprint author), ARS, USDA, Gainesville, FL 32608 USA. EM dewayne.shoemaker@ars.usda.gov NR 15 TC 0 Z9 0 U1 10 U2 11 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2016 VL 99 IS 1 BP 117 EP 119 PG 3 WC Entomology SC Entomology GA DH1MP UT WOS:000372548900021 ER PT J AU Duncan, JG Steininger, MS Wright, SA Wheeler, GS AF Duncan, J. G. Steininger, M. S. Wright, S. A. Wheeler, G. S. TI Host range of Caloptilia triadicae (Lepidoptera: Gracillariidae): an adventive herbivore of Chinese tallowtree (Malpighiales: Euphorbiaceae) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Triadica sebifera; Gracillariidae; biological control of weeds; multiple-choice test ID BIOLOGICAL-CONTROL AGENT; TREE SAPIUM-SEBIFERUM; UNITED-STATES AB An adventive herbivore of the invasive weed Chinese tallowtree (Triadica sebifera [L.] Small; Malpighiales: Euphorbiaceae) first appeared in North America in 2004. Identified as a Caloptilia triadicae Davis (Lepidoptera: Gracillariidae), this leaf miner was found damaging T. sebifera saplings. In Gainesville, Florida, we exposed naturalized populations of C. triadicae to Chinese tallowtree and related plant species in multiple-choice tests. Our findings indicated that the larvae of this species had a narrow host range as they readily fed and completed development on Chinese tallowtree leaves. The only exception to this specificity was larval feeding and development on the native oysterwood, Gymnanthes lucida Sw. (Malpighiales: Euphorbiaceae). Naturalized populations of C. triadicae were also found in Miami-Dade and Broward Counties, Florida, feeding and developing on ornamental populations of G. lucida. C1 [Duncan, J. G.; Steininger, M. S.; Wright, S. A.] ARS, USDA, Invas Plant Res Lab, Gainesville, FL 32608 USA. [Wheeler, G. S.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. RP Wheeler, GS (reprint author), ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. EM greg.wheeler@ars.usda.gov NR 18 TC 0 Z9 0 U1 1 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD MAR PY 2016 VL 99 IS 1 BP 142 EP 145 PG 4 WC Entomology SC Entomology GA DH1MP UT WOS:000372548900030 ER PT J AU McHugh, T Niemira, BA AF McHugh, Tara Niemira, Brendan A. TI Cold Plasma Technology Close-Up SO FOOD TECHNOLOGY LA English DT Editorial Material C1 [McHugh, Tara] USDA ARS, Albany, CA USA. [Niemira, Brendan A.] USDA, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, Wyndmoor, PA USA. RP McHugh, T (reprint author), USDA ARS, Albany, CA USA.; Niemira, BA (reprint author), USDA, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, Wyndmoor, PA USA. EM tara.mchugh@ars.usda.gov; brendan.niemira@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD MAR PY 2016 VL 70 IS 3 BP 68 EP 72 PG 5 WC Food Science & Technology SC Food Science & Technology GA DH1RU UT WOS:000372563400019 ER PT J AU Cox, NA Richardson, KE Cosby, DE Berrang, ME Cason, JA Rigsby, LL Holcombe, N DeRome, L AF Cox, N. A. Richardson, K. E. Cosby, D. E. Berrang, M. E. Cason, J. A. Rigsby, L. L. Holcombe, N. DeRome, L. TI Injury and death of various Salmonella serotypes due to acidic conditions SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article ID FEED; PREENRICHMENT; RECOVERY; SAMPLES AB Acid injury could prevent detection of Salmonella in feed and feed-type samples. A previous study showed that after incubation in commonly used pre-enrichment media, mixed feeds and feed ingredients reached a pH (4.0 to 5.0), capable of injuring or killing Salmonella. Approximately 105 colony forming units (CFU) of S. Enteritidis (SE), S. Heidelberg (SH), S. Kentucky (SK), or S. Typhimurium (ST) were individually placed into 50 mL of a citrate buffer at pH of 4, 4.5, or 5.0 for 6 or 24 h at 37 degrees C. After holding at 37 degrees C, each serotype was serially diluted in sterile physiological saline and plated onto xylose lysine tergitol 4 [for injury] and nutrient agar (NA) [for death]. These plates were enumerated and percent injury and/or death determined. The injury and death confirmed that pH 4.0 was detrimental to these Salmonella serotypes. After 24 h at pH 4.0, 60% or more of all 4 serotypes were killed with 100% of S. Kentucky killed. At pH 5.0, 65 to 75% of these organisms were uninjured, with death between 15 to 20%. Researchers testing feed/feed ingredients for Salmonella may not be aware of the acidic nature of the pre-enrichment step and the subsequent injury or death of any Salmonella present, whether healthy or stressed. Current protocols that are considered the "gold standard" may not be detecting Salmonella in samples containing fermentable substrates and extraneous microorganisms, which prevent the accurate detection of Salmonella. Injury or death of a few Salmonella cells could reduce the chance of recovery from any sample. More research is needed with additional Salmonella serotypes and strains to fully understand the challenges of isolating Salmonella from feed. C1 [Cox, N. A.; Cosby, D. E.; Berrang, M. E.; Rigsby, L. L.] ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. [Richardson, K. E.; Holcombe, N.; DeRome, L.] Anitox Corp, Lawrenceville, GA 30043 USA. [Cason, J. A.] Williamsburg Lane, Athens, GA 30605 USA. RP Cosby, DE (reprint author), ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. EM douglas.cosby@ars.usda.gov NR 14 TC 1 Z9 1 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD MAR PY 2016 VL 25 IS 1 BP 62 EP 66 DI 10.3382/japr/pfv062 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA DG9VN UT WOS:000372432000008 ER PT J AU McKelvey, KS Young, MK Knotek, WL Carim, KJ Wilcox, TM Padgett-Stewart, TM Schwartz, MK AF McKelvey, K. S. Young, M. K. Knotek, W. L. Carim, K. J. Wilcox, T. M. Padgett-Stewart, T. M. Schwartz, M. K. TI Sampling large geographic areas for rare species using environmental DNA: a study of bull trout Salvelinus confluentus occupancy in western Montana SO JOURNAL OF FISH BIOLOGY LA English DT Article DE distribution; eDNA; genetics; occupancy; quantitative PCR ID GREAT CRESTED NEWT; ABUNDANCE; STREAMS; EDNA AB This study tested the efficacy of environmental DNA (eDNA) sampling to delineate the distribution of bull trout Salvelinus confluentus in headwater streams in western Montana, U.S.A. Surveys proved fast, reliable and sensitive: 124 samples were collected across five basins by a single crew in c. 8 days. Results were largely consistent with past electrofishing, but, in a basin where S. confluentus were known to be scarce, eDNA samples indicated that S. confluentus were more broadly distributed than previously thought. C1 [McKelvey, K. S.; Young, M. K.] US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. [Knotek, W. L.] Montana Fish Wildlife & Pk, 3201 Spurgin Rd, Missoula, MT 59804 USA. [Carim, K. J.; Wilcox, T. M.; Schwartz, M. K.] US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. [Wilcox, T. M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Padgett-Stewart, T. M.] MCPS, Hellgate High Sch, 900 South Higgins Ave, Missoula, MT 59801 USA. RP McKelvey, KS (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. EM kmkelvey@fs.fed.us FU U.S. Forest Service's Northern Region and Rocky Mountain Research Station; NSF Graduate Research Fellowship [DGE-1313190] FX This study was funded by the U.S. Forest Service's Northern Region and Rocky Mountain Research Station. T.M.W. is supported by an NSF Graduate Research Fellowship (Grant No. DGE-1313190). Additionally, the authors would like to acknowledge W. Lowe and K. Zarn for their reviews of an earlier draft. NR 22 TC 4 Z9 4 U1 23 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1112 EI 1095-8649 J9 J FISH BIOL JI J. Fish Biol. PD MAR PY 2016 VL 88 IS 3 BP 1215 EP 1222 DI 10.1111/jfb.12863 PG 8 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DG8CT UT WOS:000372310700013 PM 26762274 ER PT J AU Cushman, SA AF Cushman, Samuel A. TI Calculating the configurational entropy of a landscape mosaic SO LANDSCAPE ECOLOGY LA English DT Article DE Entropy; Landscape; Configuration; Composition; Thermodynamics ID ECOLOGY; METRICS; RULE AB Background Applications of entropy and the second law of thermodynamics in landscape ecology are rare and poorly developed. This is a fundamental limitation given the centrally important role the second law plays in all physical and biological processes. A critical first step to exploring the utility of thermodynamics in landscape ecology is to define the configurational entropy of a landscape mosaic. In this paper I attempt to link landscape ecology to the second law of thermodynamics and the entropy concept by showing how the configurational entropy of a landscape mosaic may be calculated. Result I begin by drawing parallels between the configuration of a categorical landscape mosaic and the mixing of ideal gases. I propose that the idea of the thermodynamic microstate can be expressed as unique configurations of a landscape mosaic, and posit that the landscape metric Total Edge length is an effective measure of configuration for purposes of calculating configurational entropy. Conclusions I propose that the entropy of a given landscape configuration can be calculated using the Boltzmann equation. Specifically, the configurational entropy can be defined as the logarithm of the number of ways a landscape of a given dimensionality, number of classes and proportionality can be arranged (microstates) that produce the observed amount of total edge (macrostate). C1 [Cushman, Samuel A.] US Forest Serv, USDA, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP Cushman, SA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM scushman@fs.fed.us NR 22 TC 0 Z9 0 U1 2 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAR PY 2016 VL 31 IS 3 BP 481 EP 489 DI 10.1007/s10980-015-0305-2 PG 9 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DG8FW UT WOS:000372319400002 ER PT J AU Rogers, PC AF Rogers, Paul C. TI Saving Pando Humans are taking measured steps to rejuvenate an ailing giant. SO NATURAL HISTORY LA English DT Article C1 [Rogers, Paul C.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Rogers, Paul C.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Rogers, Paul C.] US Forest Serv, Washington, DC USA. RP Rogers, PC (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA.; Rogers, PC (reprint author), Utah State Univ, Ctr Ecol, Logan, UT 84322 USA.; Rogers, PC (reprint author), US Forest Serv, Washington, DC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU NATURAL HISTORY MAGAZINE PI NEW YORK PA 36 WEST 25TH STREET, FIFTH FLOOR, NEW YORK, NY 10010 USA SN 0028-0712 J9 NAT HIST JI Nat. Hist. PD MAR PY 2016 VL 124 IS 2 BP 32 EP 37 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DH0LN UT WOS:000372475800006 ER PT J AU Yuan, X Cao, J He, XN Serra, R Qu, J Cao, X Yang, SY AF Yuan, Xue Cao, Jay He, Xiaoning Serra, Rosa Qu, Jun Cao, Xu Yang, Shuying TI Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation SO NATURE COMMUNICATIONS LA English DT Article ID INTRAFLAGELLAR TRANSPORT PROTEIN; ASPHYXIATING THORACIC DYSTROPHY; ACTIN STRESS FIBERS; PRIMARY CILIUM; BONE-FORMATION; STEM-CELLS; RUNX2 EXPRESSION; MICE; TRAFFICKING; ACTIVATION AB Intraflagellar transport proteins (IFT) are required for hedgehog (Hh) signalling transduction that is essential for bone development, however, how IFT proteins regulate Hh signalling in osteoblasts (OBs) remains unclear. Here we show that deletion of ciliary IFT80 in OB precursor cells (OPC) in mice results in growth retardation and markedly decreased bone mass with impaired OB differentiation. Loss of IFT80 blocks canonical Hh-Gli signalling via disrupting Smo ciliary localization, but elevates non-canonical Hh-Gai-RhoA-stress fibre signalling by increasing Smo and G alpha i binding. Inhibition of RhoA and ROCK activity partially restores osteogenic differentiation of IFT80-deficient OPCs by inhibiting non-canonical Hh-RhoA-Cofilin/MLC2 signalling. Cytochalasin D, an actin destabilizer, dramatically restores OB differentiation of IFT80-deficient OPCs by disrupting actin stress fibres and promoting cilia formation and Hh-Gli signalling. These findings reveal that IFT80 is required for OB differentiation by balancing between canonical Hh-Gli and non-canonical Hh-Gai-RhoA pathways and highlight IFT80 as a therapeutic target for craniofacial and skeletal abnormalities. C1 [Yuan, Xue; He, Xiaoning; Yang, Shuying] SUNY Buffalo, Sch Dent Med, Dept Oral Biol, 3435 Main St, Buffalo, NY 14214 USA. [Cao, Jay] USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Serra, Rosa] Univ Alabama Birmingham, Dept Cell Dev & Integrat Biol, Birmingham, AL 35294 USA. [Qu, Jun] SUNY Buffalo, Dept Pharmaceut Sci, Buffalo, NY 14214 USA. [Cao, Xu] Johns Hopkins Univ, Dept Orthopaed Surg, Sch Med, Baltimore, MD 21205 USA. [Yang, Shuying] SUNY Buffalo, Dev Genom Grp, New York State Ctr Excellence Bioinformat & Life, Buffalo, NY 14203 USA. RP Yang, SY (reprint author), SUNY Buffalo, Sch Dent Med, Dept Oral Biol, 3435 Main St, Buffalo, NY 14214 USA.; Yang, SY (reprint author), SUNY Buffalo, Dev Genom Grp, New York State Ctr Excellence Bioinformat & Life, Buffalo, NY 14203 USA. EM sy47@buffalo.edu OI Yuan, Xue/0000-0002-8063-9431 FU National Cancer Institute (NCI) [P30CA016056]; National Institute of Dental and Craniofacial Research; National Institute of Arthritis and Musculoskeletal and Skin Diseases; National Institutes of Health [DE023105, AR066101, AG048388] FX We thank Dr. Kristina Wasson-Blader, Miss. Stacy Scheuneman and Mr. Andrew Ng for editing the manuscript. We thank Dr. Aimee B. Stablewski and Gene Targeting and Transgenic Shared Resource at Roswell Park Cancer Institute (supported by the National Cancer Institute (NCI) grant P30CA016056), for technical assistance with the ES cell injections for generating IFT80 floxed mice. Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research and the National Institute of Arthritis and Musculoskeletal and Skin Diseases, part of the National Institutes of Health, under Award Numbers DE023105, AR066101 and AG048388 to S.Y. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. NR 70 TC 1 Z9 2 U1 4 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAR PY 2016 VL 7 DI 10.1038/ncomms11024 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH0UE UT WOS:000372498800001 PM 26996322 ER PT J AU Coulston, JW Blinn, CE Thomas, VA Wynne, RH AF Coulston, John W. Blinn, Christine E. Thomas, Valerie A. Wynne, Randolph H. TI Approximating Prediction Uncertainty for Random Forest Regression Models SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID MACHINE LEARNING ALGORITHMS; LAND-COVER DATABASE; UNITED-STATES; INFORMATION; BIOMASS AB Machine learning approaches such as random forest have increased for the spatial modeling and mapping of continuous variables. Random forest is a non-parametric ensemble approach, and unlike traditional regression approaches there is no direct quantification of prediction error. Understanding prediction uncertainty is important when using model-based continuous maps as inputs to other modeling applications such as fire modeling. Here we use a Monte Carlo approach to quantify prediction uncertainty for random forest regression models. We test the approach by simulating maps of dependent and independent variables with known characteristics and comparing actual errors with prediction errors. Our approach produced conservative prediction intervals across most of the range of predicted values. However, because the Monte Carlo approach was data driven, prediction intervals were either too wide or too narrow in sparse parts of the prediction distribution. Overall, our approach provides reasonable estimates of prediction uncertainty for random forest regression models. C1 [Coulston, John W.] USDA Forest Serv, Southern Res Stn, Blacksburg, VA USA. [Blinn, Christine E.; Thomas, Valerie A.; Wynne, Randolph H.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. RP Coulston, JW (reprint author), USDA Forest Serv, Southern Res Stn, Blacksburg, VA USA. EM jcoulston@fs.fed.us NR 31 TC 0 Z9 0 U1 6 U2 12 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD MAR PY 2016 VL 82 IS 3 BP 189 EP 197 PG 9 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA DH1QH UT WOS:000372559500004 ER PT J AU Ruefenacht, B AF Ruefenacht, Bonnie TI Comparison of Three Landsat TM Compositing Methods: A Case Study Using Modeled Tree Canopy Cover SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID CONTERMINOUS UNITED-STATES; THEMATIC MAPPER DATA; FOREST; DATABASE; IMAGERY; CLASSIFICATION; MODIS AB Landsat imagery mosaics developed using model II regression have been shown to successfully model percent tree-canopy cover (PTCC). Creating model II regression mosaics, however, is a time-consuming, manual process. The objective of this study is to evaluate the effectiveness of using more easily automated image composites techniques, such as median Landsat-5 image composites or maximum NDVI Landsat-5 image composites, as alternatives to model II regression mosaics for the modeling of PTCC. This study found all composite types were effective in modeling PTCC, but the maximum NDVI composites included anomalies, clouds, shadows, and tended to be pixelated, whereas the median composites and the model II regression mosaics had none of these issues. The median composite procedure is automated and was found to be an effective approach to statistically reduce a much larger ensemble of images on a pixel basis to create images suitable for vegetation modeling. C1 [Ruefenacht, Bonnie] USDA Forest Serv, Remote Sensing Applicat Ctr, 2222 West 2300 South, Salt Lake City, UT 84119 USA. RP Ruefenacht, B (reprint author), USDA Forest Serv, Remote Sensing Applicat Ctr, 2222 West 2300 South, Salt Lake City, UT 84119 USA. EM bruefenacht@fs.fed.us NR 29 TC 0 Z9 0 U1 8 U2 9 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD MAR PY 2016 VL 82 IS 3 BP 199 EP 211 PG 13 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA DH1QH UT WOS:000372559500005 ER PT J AU Cordell, S Ostertag, R Michaud, J Warman, L AF Cordell, Susan Ostertag, Rebecca Michaud, Jene Warman, Laura TI Quandaries of a decade-long restoration experiment trying to reduce invasive species: beat them, join them, give up, or start over? SO RESTORATION ECOLOGY LA English DT Article DE Hawaii; hybrid ecosystems; invasive species; long-term research; tropical lowland wet forest ID TERM ECOLOGICAL RESEARCH; FOREST RESTORATION; NITROGEN DYNAMICS; DRY FOREST; SEED BANKS; ECOSYSTEM; HAWAII; CONSEQUENCES; MANAGEMENT; REMOVAL AB We evaluate the outcomes and consequences of a decade-long restoration project in a Hawaiian lowland wet forest as they relate to long-term management actions. Our initial study was designed both to promote native biodiversity and to develop knowledge that would enable land management agencies to restore invaded forests. Our premise of success followed the prevalent perception that short-term management, such as removal of invasive species, ideally translates into long-term and sustainable restoration. We were therefore disappointed and perhaps discouraged in our resultslittle recovery of native biodiversity despite ongoing and labor-intensive management. Not only did we fail to return the invaded forest to a native-dominated system but also our efforts lead to recruitment of new non-native species assemblages. The sobering truth of many restoration projects in Hawaii and elsewhere is that we can never completely walk away and consider the job finished, or we have to accept that some ecosystems cannot be returned to an all-native state. Essentially, costs of restoration may outweigh the accomplishment. This setback gave us an opportunity to reconsider and modify our initial approach. By starting over with a new direction using both native and non-invasive but non-native species, we have adopted a new philosophy of join them. In our revision, we changed the players in the game by following invasive species removal with outplantings of native and non-invasive non-native species that will functionally fill missing roles in the ecosystem. We link social interest in the new experiment to changing attitudes about naturalness. C1 [Cordell, Susan; Warman, Laura] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. [Ostertag, Rebecca] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. [Michaud, Jene] Univ Hawaii, Dept Geol, Hilo, HI 96720 USA. RP Cordell, S (reprint author), US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. EM scordell01@fs.fed.us RI Warman, Laura/C-6651-2011 OI Warman, Laura/0000-0001-7339-749X FU NSF EPSCoR [0237065, 0554657, 0903833]; NSF [TCUP 0223040, 0546868]; NSF REU [0139379, 1005186, 0453630]; Strategic Environmental Research and Development Program [RC-2117] FX This research was funded and facilitated through NSF EPSCoR Awards 0237065, 0554657, and 0903833, NSF TCUP 0223040, NSF CAREER 0546868, NSF REU Awards 0139379, 1005186, and 0453630, and Strategic Environmental Research and Development Program for funding (Project RC-2117). The authors recognize staff, students, and interns from the University of Hawaii and the USDA Forest Service Institute of Pacific Islands Forestry for logistical and technical support. We thank our partners in the Hawaii Army National Guard Environmental Office (currently, Angela Kieran-Vast and Craig Blaisdell) and staff at Keaukaha Military Reservation for facilitating the establishment of the Liko Na Pilina project. NR 40 TC 1 Z9 1 U1 28 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD MAR PY 2016 VL 24 IS 2 BP 139 EP 144 DI 10.1111/rec.12321 PG 6 WC Ecology SC Environmental Sciences & Ecology GA DG7SB UT WOS:000372282900001 ER PT J AU Chivers, IH Jones, TA Broadhurst, LM Mott, IW Larson, SR AF Chivers, Ian H. Jones, Thomas A. Broadhurst, Linda M. Mott, Ivan W. Larson, Steve R. TI The merits of artificial selection for the development of restoration-ready plant materials of native perennial grasses SO RESTORATION ECOLOGY LA English DT Review DE functional traits; genetic diversity; genetic drift; genetic shift; recurrent selection; seed shattering; seedling establishment; trade-offs ID DANTHONIA-RICHARDSONII CASHMORE; RUSSIAN WILD RYEGRASS; INDIAN RICEGRASS; SEED PRODUCTION; BLUE GRAMA; ECOLOGICAL RESTORATION; BLUEBUNCH WHEATGRASS; STAND ESTABLISHMENT; GENETIC DISSECTION; NATURAL-SELECTION AB Although seed harvested from remnant, wildland perennial-grass populations can be used for restoration in humid and subhumid temperate regions, seed harvested in semiarid and arid environments is often of low quality and highly variable in quantity. In addition, ongoing harvest of indigenous populations can be unsustainable, especially for those that are small. In such environments, dependable and repeatable broad-scale restoration of degraded grasslands requires sufficient and consistent supplies of reliable, cost-effective seed sources that can only result from intensively managed cultivated stands. But does the harvest of intensively managed seed-production fields inadvertently compromise genetic diversity, thereby adversely affecting the restoration outcome? That is, are seed-production systems a part of the solution for restoration, or do they create new unintended management issues? This article discusses the potential impacts of cultivated seed-production systems and recurrent artificial selection for specific traits on genetic integrity and performance of native-species perennial-grass populations. Although genetic shift resulting from cultivated perennial-grass seed production may be inevitable, genetic shifts that change phenological expression may be limited in genotypes that exhibit high seed retention. Artificial selection can improve plant material performance on the often-harsh conditions of restoration sites, but sufficiently high-effective population sizes (N-e) must be maintained to conserve genetic diversity, thereby precluding the inbreeding depression that can compromise plant performance. Potentially useful traits of native perennial-grass species that respond to artificial selection include seed production, seed retention, seedling establishment, competitive ability against weeds, and herbicide tolerance. Potential trade-offs between traits should also be considered to avoid undesirable inadvertent responses to selection. C1 [Chivers, Ian H.] Native Seeds Pty Ltd, 34-148 Chesterville Rd, Cheltenham, Vic 3192, Australia. [Jones, Thomas A.; Mott, Ivan W.; Larson, Steve R.] USDA ARS, Forage & Range Res Lab, 696 North 1100 East, Logan, UT 84322 USA. [Broadhurst, Linda M.] CSIRO Natl Res Collect Australia, CSIRO GPO Box 1600, Canberra, ACT 2601, Australia. RP Chivers, IH (reprint author), Native Seeds Pty Ltd, 34-148 Chesterville Rd, Cheltenham, Vic 3192, Australia. EM ian@nativeseeds.com.au FU BHP Billiton Iron Ore Community Development Project of the Restoration Seedbank Initiative [8600048550] FX We wish to thank T. Monaco for clarifying issues regarding biomass allocation and morphological traits. This article is a direct outcome of discussions held during the Restoration Seedbank Initiative's Think Tank Workshop focussing on "seed enablement technologies and recreating the recruitment niche in degraded environments." Logistical and financial support was provided by a BHP Billiton Iron Ore Community Development Project (contract no. 8600048550) under the auspices of the Restoration Seedbank Initiative, a partnership between BHP Billiton Iron Ore, The University of Western Australia, and the Botanic Gardens and Parks Authority. NR 80 TC 3 Z9 3 U1 21 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD MAR PY 2016 VL 24 IS 2 BP 174 EP 183 DI 10.1111/rec.12323 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DG7SB UT WOS:000372282900006 ER PT J AU Dorr, BS Hanson-Dorr, KC Barras, SC DeVault, TL AF Dorr, Brian S. Hanson-Dorr, Katie C. Barras, Scott C. DeVault, Travis L. TI Reproductive Characteristics of Double-crested Cormorants (Phalacrocorax auritus) in the Eastern United States: Demographic Information for an Intensely Managed Species SO WATERBIRDS LA English DT Article DE breeding status; culling; Double-crested Cormorant; management; nesting colony; Phalacrocorax auritus; populations ID FOLLICLES AB Double-crested Cormorants (Phalacrocorax auritus) are intensely managed through culling in North America to reduce presumed damage to commercial and natural resources. To evaluate this management, there is a critical need to understand the reproductive biology of Double-crested Cormorants. Gonadal development, reproductive potential and breeding status were determined by necropsy for 1,781 DOuble-crested Cormorants salvaged each month of the year from control programs in their wintering and breeding ranges in seven States in the USA. Gonadal development of males peaked earlier in the year than females. Mean and maximum reproductive potential was five and 13 ovum, respectively. The average proportion of non-breeding female Double-crested Cormorants culled from breeding colonies was 14.9% (n = 202) and from foraging flocks on the breeding grounds was 22.1% (n = 358). This demographic information should be considered when evaluating and modeling effects of Double-crested Cormorant management in North America. C1 [Dorr, Brian S.; Hanson-Dorr, Katie C.] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Mississippi Field Stn, POB 6099, Mississippi State, MS 39762 USA. [Barras, Scott C.] USDA, Wildlife Serv, POB 130, Moseley, VA 23120 USA. [DeVault, Travis L.] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. RP Dorr, BS (reprint author), USDA, Wildlife Serv, Natl Wildlife Res Ctr, Mississippi Field Stn, POB 6099, Mississippi State, MS 39762 USA. EM brian.s.dorr@aphis.usda.gov OI Hanson-Dorr, Katie/0000-0003-4559-938X FU U.S. Department of Agriculture, Wildlife Services, State programs; National Wildlife Research Center FX We thank the U.S. Department of Agriculture, Wildlife Services, State programs, in particular Elizabeth Cranker and Josh Friers, and the National Wildlife Research Center for logistical support and funding. We thank M. Tobin and two anonymous reviewers for their comments on this manuscript. This research was conducted under U.S. Department of the Interior, U.S. Geological Survey, Federal Bird Marking and Salvage Permit No. 20873. Use of avian subjects was approved by the USDA-WS National Wildlife Research Center's Institutional Animal Care and Use Committee (QA-1398). NR 15 TC 0 Z9 0 U1 3 U2 5 PU WATERBIRD SOC PI WASHINGTON PA NATL MUSEUM NATURAL HISTORY SMITHSONIAN INST, WASHINGTON, DC 20560 USA SN 1524-4695 EI 1938-5390 J9 WATERBIRDS JI Waterbirds PD MAR PY 2016 VL 39 IS 1 BP 81 EP 85 PG 5 WC Ornithology SC Zoology GA DG9BE UT WOS:000372377000009 ER PT J AU Hair, AB Peluso, AM Hawthorne, KM Perez, J Smith, DP Khan, JY O'Donnell, A Powers, RJ Lee, ML Abrams, SA AF Hair, Amy B. Peluso, Allison M. Hawthorne, Keli M. Perez, Jose Smith, Denise P. Khan, Janine Y. O'Donnell, Andrea Powers, Richard J. Lee, Martin L. Abrams, Steven A. TI Beyond Necrotizing Enterocolitis Prevention: Improving Outcomes with an Exclusive Human Milk-Based Diet SO BREASTFEEDING MEDICINE LA English DT Article ID EXTREMELY PREMATURE-INFANTS; PRETERM INFANTS; PRODUCTS C1 [Hair, Amy B.; Peluso, Allison M.; Hawthorne, Keli M.; Abrams, Steven A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Sect Neonatol,Baylor Coll Med,Texas C, Houston, TX 77030 USA. [Perez, Jose; Smith, Denise P.] Winnie Palmer Hosp Women & Babies, Dept Neonatol, Orlando, FL USA. [Khan, Janine Y.] Northwestern Univ, Feinberg Sch Med, Dept Pediat, Chicago, IL USA. [O'Donnell, Andrea] NW Mem Hosp, Dept Clin Nutr Serv, Chicago, IL 60611 USA. [Powers, Richard J.] Good Samaritan Hosp, Dept Neonatol, San Jose, CA USA. [Lee, Martin L.] Prolacta Biosci, City Of Industry, CA USA. RP Hair, AB (reprint author), Texas Childrens Hosp, Dept Pediat, Sect Neonatol, 6621 Fannin St,Suite W6104, Houston, TX 77030 USA. EM abhair@texaschildrenshospital.org OI Hair, Amy/0000-0001-7570-0667; Abrams, Steven/0000-0003-4972-9233 NR 16 TC 3 Z9 4 U1 0 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1556-8253 EI 1556-8342 J9 BREASTFEED MED JI Breastfeed. Med. PD MAR 1 PY 2016 VL 11 IS 2 BP 70 EP 74 PG 5 WC Obstetrics & Gynecology; Pediatrics SC Obstetrics & Gynecology; Pediatrics GA DG1NH UT WOS:000371834300006 PM 26789484 ER PT J AU Guo, M Mishra, A Buchanan, RL Dubey, JP Hill, DE Gamble, HR Jones, JL Pradhan, AK AF Guo, Miao Mishra, Abhinav Buchanan, Robert L. Dubey, Jitender P. Hill, Dolores E. Gamble, H. Ray Jones, Jeffrey L. Pradhan, Abani K. TI A Systematic Meta-Analysis of Toxoplasma gondii Prevalence in Food Animals in the United States SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID QUANTITATIVE RISK-ASSESSMENT; HUMAN CONSUMPTION; DAIRY GOATS; SWINE FARMS; TRICHINELLA-SPIRALIS; DOMESTIC-ANIMALS; INFECTION; PIGS; ANTIBODIES; SHEEP AB Toxoplasma gondii is a widely distributed protozoan parasite. The Centers for Disease Control and Prevention reported that T. gondii is one of three pathogens (along with Salmonella and Listeria), that together account for >70% of all deaths due to foodborne illness in the United States. Food animals are reservoirs for T. gondii and act as one of the sources for parasite transmission to humans. Based on limited population-based data, the Food and Agriculture Organization/World Health Organization estimated that approximately 22% of human T. gondii infections are meatborne. The objective of the current study was to conduct a systematic meta-analysis to provide a precise estimation of T. gondii infection prevalence in food animals produced in the United States. Four databases were searched to collect eligible studies. Prevalence was estimated in six animal categories (confinement-raised market pigs, confinement-raised sows, non-confinement-raised pigs, lamb, goats, and non-confinement-raised chickens) by a quality-effects model. A wide variation in prevalence was observed in each animal category. Animals raised outdoors or that have outdoor access had a higher prevalence as compared with animals raised indoors. T. gondii prevalence in non-confinement-raised pigs ranked the highest (31.0%) followed by goats (30.7%), non-confinement-raised chickens (24.1%), lambs (22.0%), confinement-raised sows (16.7%), and confinement-raised market pigs (5.6%). These results indicate that T. gondii-infected animals are a food safety concern. The computed prevalence can be used as an important input in quantitative microbial risk assessment models to further predict public health burden. C1 [Guo, Miao; Mishra, Abhinav; Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Dept Nutr & Food Sci, Skinner Bldg Room 0112, College Pk, MD 20742 USA. [Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Dubey, Jitender P.; Hill, Dolores E.] USDA, Anim Parasit Dis Lab, Agr Res Serv, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Gamble, H. Ray] Natl Acad Sci, 2101 Constitut Ave Nw, Washington, DC 20418 USA. [Jones, Jeffrey L.] Ctr Dis Control & Prevent, Atlanta, GA USA. RP Pradhan, AK (reprint author), Univ Maryland, Dept Nutr & Food Sci, Skinner Bldg Room 0112, College Pk, MD 20742 USA. EM akp@umd.edu OI Mishra, Abhinav/0000-0001-9214-0745 FU USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative [2012-67005-19611] FX This work was supported through a grant from the USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (award 2012-67005-19611). NR 74 TC 3 Z9 3 U1 3 U2 13 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAR 1 PY 2016 VL 13 IS 3 BP 109 EP 118 DI 10.1089/fpd.2015.2070 PG 10 WC Food Science & Technology SC Food Science & Technology GA DG2FN UT WOS:000371882100001 PM 26854596 ER PT J AU Franklin, AM Aga, DS Cytryn, E Durso, LM McLain, JE Pruden, A Roberts, MC Rothrock, MJ Snow, DD Watson, JE Dungan, RS AF Franklin, Alison M. Aga, Diana S. Cytryn, Eddie Durso, Lisa M. McLain, Jean E. Pruden, Amy Roberts, Marilyn C. Rothrock, Michael J., Jr. Snow, Daniel D. Watson, John E. Dungan, Robert S. TI Antibiotics in Agroecosystems: Introduction to the Special Section SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID TETRACYCLINE RESISTANCE GENES; CHROMATOGRAPHY-MASS SPECTROMETRY; ANTIMICROBIAL RESISTANCE; WASTE-WATER; RISK-ASSESSMENT; MICROBIAL COMMUNITY; TERRESTRIAL ENVIRONMENT; PSEUDOMONAS-AERUGINOSA; VETERINARY ANTIBIOTICS; AGRICULTURAL SOILS AB The presence of antibiotic drug residues, antibiotic resistant bacteria, and antibiotic resistance genes in agroecosystems has become a significant area of research in recent years and is a growing public health concern. While antibiotics are used in both human medicine and agricultural practices, the majority of their use occurs in animal production where historically they have been used for growth promotion, in addition to the prevention and treatment of disease. The widespread use of antibiotics and the application of animal wastes to agricultural lands play major roles in the introduction of antibiotic-related contamination into the environment. Overt toxicity in organisms directly exposed to antibiotics in agroecosystems is typically not a major concern because environmental concentrations are generally lower than therapeutic doses. However, the impacts of introducing antibiotic contaminants into the environment are unknown, and concerns have been raised about the health of humans, animals, and ecosystems. Despite increased research focused on the occurrence and fate of antibiotics and antibiotic resistance over the past decade, standard methods and practices for analyzing environmental samples are limited and future research needs are becoming evident. To highlight and address these issues in detail, this special collection of papers was developed with a framework of five core review papers that address the (i) overall state of science of antibiotics and antibiotic resistance in agroecosystems using a causal model, (ii) chemical analysis of antibiotics found in the environment, (iii) need for background and baseline data for studies of antibiotic resistance in agroecosystems with a decision-making tool to assist in designing research studies, as well as (iv) culture- and (v) molecular-based methods for analyzing antibiotic resistance in the environment. With a focus on the core review papers, this introduction summarizes the current state of science for analyzing antibiotics and antibiotic resistance in agroecosystems, discusses current knowledge gaps, and develops future research priorities. This introduction also contains a glossary of terms used in the core reivew papers of this special section. The purpose of the glossary is to provide a common terminology that clearly characterizes the concepts shared throughout the narratives of each review paper. C1 [Franklin, Alison M.; Watson, John E.] Penn State Univ, Dept Ecosyst Sci & Management, 116 ASI Bldg, University Pk, PA 16802 USA. [Aga, Diana S.] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA. [Cytryn, Eddie] Agr Res Org, Volcani Ctr, Inst Soil Water & Environm Sci, POB 6, IL-50250 Bet Dagan, Israel. [Durso, Lisa M.] USDA ARS, Agroecosyst Management Res Unit, 251 Filley Hall,UNL East Campus, Lincoln, NE 68583 USA. [McLain, Jean E.] Univ Arizona, Water Resources Res Ctr, 350 N Campbell Ave, Tucson, AZ 85719 USA. [Pruden, Amy] Virginia Tech, Civil & Environm Engn, 403 Durham Hall, Blacksburg, VA 24061 USA. [Roberts, Marilyn C.] Univ Washington, Environm & Occupat Hlth Serv, Box 357234,1959 NE Pacific St, Seattle, WA 98195 USA. [Rothrock, Michael J., Jr.] USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. [Snow, Daniel D.] Univ Nebraska, Nebraska Water Ctr, Sch Nat Resources, Lincoln, NE USA. [Dungan, Robert S.] USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. RP Franklin, AM (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, 116 ASI Bldg, University Pk, PA 16802 USA.; Dungan, RS (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM alisonfranklin@psu.edu; robert.dungan@ars.usda.gov FU Agriculture and Food Research Initiative Competitive from USDA National Institute of Food and Agriculture [2013-68003-21256] FX We thank all of the authors who contributed papers to this special section, which would not have been possible without their efforts. This project was supported by the Agriculture and Food Research Initiative Competitive Grant no. 2013-68003-21256 from the USDA National Institute of Food and Agriculture. NR 151 TC 1 Z9 1 U1 13 U2 52 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 377 EP 393 DI 10.2134/jeq2016.01.0023 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900001 PM 27065385 ER PT J AU Williams-Nguyen, J Sallach, JB Bartelt-Hunt, S Boxall, AB Durso, LM McLain, JE Singer, RS Snow, DD Zilles, JL AF Williams-Nguyen, Jessica Sallach, J. Brett Bartelt-Hunt, Shannon Boxall, Alistair B. Durso, Lisa M. McLain, Jean E. Singer, Randall S. Snow, Daniel D. Zilles, Julie L. TI Antibiotics and Antibiotic Resistance in Agroecosystems: State of the Science SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID TANDEM MASS-SPECTROMETRY; WASTE-WATER IRRIGATION; ESCHERICHIA-COLI O157H7; ANTIMICROBIAL RESISTANCE; LIQUID-CHROMATOGRAPHY; HUMAN HEALTH; FOODBORNE PATHOGENS; RISK-ASSESSMENT; DRINKING-WATER; PIG-MANURE AB We propose a simple causal model depicting relationships involved in dissemination of antibiotics and antibiotic resistance in agroecosystems and potential effects on human health, functioning of natural ecosystems, and agricultural productivity. Available evidence for each causal link is briefly summarized, and key knowledge gaps are highlighted. A lack of quantitative estimates of human exposure to environmental bacteria, in general, and antibiotic-resistant bacteria, specifically, is a significant data gap hindering the assessment of effects on human health. The contribution of horizontal gene transfer to resistance in the environment and conditions that might foster the horizontal transfer of antibiotic resistance genes into human pathogens also need further research. Existing research has focused heavily on human health effects, with relatively little known about the effects of antibiotics and antibiotic resistance on natural and agricultural ecosystems. The proposed causal model is used to elucidate gaps in knowledge that must be addressed by the research community and may provide a useful starting point for the design and analysis of future research efforts. C1 [Williams-Nguyen, Jessica] Univ Washington, Dept Epidemiol, 1959 NE Pacific St,Hlth Sci Bldg F-262,Box 357236, Seattle, WA 98195 USA. [Sallach, J. Brett] Univ Nebraska, Dept Civil Engn, N104 SLNK, Lincoln, NE 68588 USA. [Bartelt-Hunt, Shannon] Univ Nebraska, Dept Civil Engn, 203B Peter Kiewit Inst, Omaha, NE 68182 USA. [Boxall, Alistair B.] Univ York, Dept Environm, York YO10 5DD, N Yorkshire, England. [Durso, Lisa M.] USDA ARS, Agroecosyst Management Res Unit, 137 Keim Hall,UNL East Campus, Lincoln, NE 68583 USA. [McLain, Jean E.] Univ Arizona, Water Resources Res Ctr, 350 N Campbell Ave, Tucson, AZ 85719 USA. [Singer, Randall S.] Univ Minnesota, Dept Vet & Biomed Sci, 1971 Commonwealth Ave, St Paul, MN 55108 USA. [Snow, Daniel D.] Univ Nebraska, Water Sci Lab, 202 Water Sci Lab,1840 North 37th St, Lincoln, NE 68583 USA. [Zilles, Julie L.] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. RP Williams-Nguyen, J (reprint author), Univ Washington, Dept Epidemiol, 1959 NE Pacific St,Hlth Sci Bldg F-262,Box 357236, Seattle, WA 98195 USA. EM jswn@uw.edu FU Agriculture and Food Research Initiative Competitive from USDA National Institute of Food and Agriculture [2013-68003-21256]; National Pork Board (Iowa, USA) FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-68003-21256 from the USDA National Institute of Food and Agriculture. Randall Singer and Jessica Williams-Nguyen were partially supported in this endeavor by the National Pork Board (Iowa, USA). NR 171 TC 6 Z9 6 U1 24 U2 48 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 394 EP 406 DI 10.2134/jeq2015.07.0336 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900002 PM 27065386 ER PT J AU Rothrock, MJ Keen, PL Cook, KL Durso, LM Franklin, AM Dungan, RS AF Rothrock, Michael J., Jr. Keen, Patricia L. Cook, Kimberly L. Durso, Lisa M. Franklin, Alison M. Dungan, Robert S. TI How Should We Be Determining Background and Baseline Antibiotic Resistance Levels in Agroecosystem Research? SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID HORIZONTAL GENE-TRANSFER; GRAM-NEGATIVE BACTERIA; ANTIMICROBIAL RESISTANCE; ACTIVATED-SLUDGE; NATURAL ENVIRONMENTS; ESCHERICHIA-COLI; TETRACYCLINE RESISTANCE; MULTIDRUG-RESISTANCE; VETERINARY MEDICINES; MANURE FERTILIZATION AB Although historically, antibiotic resistance has occurred naturally in environmental bacteria, many questions remain regarding the specifics of how humans and animals contribute to the development and spread of antibiotic resistance in agroecosystems. Additional research is necessary to completely understand the potential risks to human, animal, and ecological health in systems altered by antibiotic-resistance-related contamination. At present, analyzing and interpreting the effects of human and animal inputs on antibiotic resistance in agroecosystems is difficult, since standard research terminology and protocols do not exist for studying background and baseline levels of resistance in the environment. To improve the state of science in antibiotic-resistance-related research in agroecosystems, researchers are encouraged to incorporate baseline data within the study system and background data from outside the study system to normalize the study data and determine the potential impact of antibiotic-resistance-related determinants on a specific agroecosystem. Therefore, the aims of this review were to (i) present standard definitions for commonly used terms in environmental antibiotic resistance research and (ii) illustrate the need for research standards (normalization) within and between studies of antibiotic resistance in agroecosystems. To foster synergy among antibiotic resistance researchers, a new surveillance and decision-making tool is proposed to assist researchers in determining the most relevant and important antibiotic-resistance-related targets to focus on in their given agroecosystems. Incorporation of these components within antibiotic-resistance-related studies should allow for a more comprehensive and accurate picture of the current and future states of antibiotic resistance in the environment. C1 [Rothrock, Michael J., Jr.] USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30606 USA. [Keen, Patricia L.] Univ British Columbia, Dept Civil Engn, Vancouver, BC, Canada. [Cook, Kimberly L.] USDA ARS, Food Anim Environm Syst Res Unit, 230 Bennett Lane, Bowling Green, KY 42104 USA. [Durso, Lisa M.] USDA ARS, Agroecosyst Management Res Unit, 137 Keim Hall,UNL East Campus, Lincoln, NE 68583 USA. [Franklin, Alison M.] Penn State Univ, Dept Ecosyst Sci & Management, 420 ASI Bldg, University Pk, PA 16802 USA. [Dungan, Robert S.] USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 North 3600 East, Kimberly, ID 83341 USA. RP Rothrock, MJ (reprint author), USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30606 USA. EM Michael.Rothrock@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2013-68003-21256] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-68003-21256 from the USDA National Institute of Food and Agriculture. NR 134 TC 2 Z9 2 U1 9 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 420 EP 431 DI 10.2134/jeq2015.06.0327 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900004 PM 27065388 ER PT J AU McLain, JE Cytryn, E Durso, LM Young, S AF McLain, Jean E. Cytryn, Eddie Durso, Lisa M. Young, Suzanne TI Culture-based Methods for Detection of Antibiotic Resistance in Agroecosystems: Advantages, Challenges, and Gaps in Knowledge SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SUSCEPTIBILITY TESTING METHODS; MUELLER-HINTON AGAR; ANTIMICROBIAL SUSCEPTIBILITY; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; BROTH MICRODILUTION; PSEUDOMONAS-AERUGINOSA; CAMPYLOBACTER-JEJUNI; METAGENOMIC ANALYSIS; ENTEROCOCCUS SPP. AB Various culture-based methodologies are used in assessment of antibiotic resistance in samples collected in agroecosystems. Culture-based methods commonly involve isolating target bacteria on general or selective media and assessing growth in response to specific concentrations of antibiotics. The advantages of culture-based methods are multifold. In particular, isolation of bacteria is key to understanding phenotypic characteristics of isolates and their resistance patterns, and most national and international antibiotic resistance monitoring projects are isolate based. This review covers current knowledge of bacterial groups and antibiotics commonly targeted in resistance studies using bacterial culture and discusses the range in methods used, data interpretation, and factors supporting and confounding the use of culture-based methods in assessment of antibiotic resistance. Gaps in knowledge related to study design and resistance databases are discussed. Finally, a case is made for the integration of culture-based and molecular methods to better inform our understanding of antibiotic resistance in agroecosystems. C1 [McLain, Jean E.] Univ Arizona, Water Resources Res Ctr, 350 N Campbell Ave, Tucson, AZ 85719 USA. [Cytryn, Eddie] Agr Res Org, Volcani Ctr, Inst Soil Water & Environm Sci, POB 6, IL-50250 Bet Dagan, Israel. [Durso, Lisa M.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Room 121 Keim Hall, Lincoln, NE 68583 USA. [Young, Suzanne] Univ S Florida, Dept Integrat Biol, 4202 E Fowlers Ave SCA110, Tampa, FL 33604 USA. RP McLain, JE (reprint author), Univ Arizona, Water Resources Res Ctr, 350 N Campbell Ave, Tucson, AZ 85719 USA. EM mclainj@email.arizona.edu FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2013-68003-21256] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-68003-21256 from the USDA National Institute of Food and Agriculture. NR 101 TC 3 Z9 3 U1 4 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 432 EP 440 DI 10.2134/jeq2015.06.0317 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900005 PM 27065389 ER PT J AU Luby, E Ibekwe, AM Zilles, J Pruden, A AF Luby, Elizabeth Ibekwe, A. Mark Zilles, Julie Pruden, Amy TI Molecular Methods for Assessment of Antibiotic Resistance in Agricultural Ecosystems: Prospects and Challenges SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID MOBILE GENETIC ELEMENTS; SWINE PRODUCTION FACILITIES; SOIL BACTERIAL COMMUNITY; GRAM-POSITIVE BACTERIA; WASTE-WATER TREATMENT; HOST-RANGE PLASMIDS; TETRACYCLINE RESISTANCE; NATURAL TRANSFORMATION; FUNCTIONAL METAGENOMICS; AZOTOBACTER-VINELANDII AB Agricultural ecosystems are of special interest for monitoring the potential for antibiotic resistance to spread through the environment and contribute to human exposure. Molecular methods, which target DNA, RNA, and other molecular components of bacterial cells, present certain advantages for characterizing and quantifying markers of antibiotic resistance and their horizontal gene transfer. These include rapid, unambiguous detection of targets; consistent results; and avoidance of culture bias. However, molecular methods are also subject to limitations that are not always clearly addressed or taken into consideration in the interpretation of scientific data. In particular, DNA-based methods do not directly assess viability or presence within an intact bacterial host, but such information may be inferred based on appropriate experimental design or in concert with complementary methods. The purpose of this review is to provide an overview of existing molecular methods for tracking antibiotic resistance in agricultural ecosystems, to define their strengths and weaknesses, and to recommend a path forward for future applications of molecular methods and standardized reporting in the literature. This will guide research along the farm-to-fork continuum and support comparability of the growing number of studies in the literature in a manner that informs management decisions and policy development. C1 [Luby, Elizabeth] Iowa State Univ, Agr & Biosyst Engn, Ames, IA 50011 USA. [Ibekwe, A. Mark] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Zilles, Julie] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. [Pruden, Amy] Virginia Tech, Dept Civil & Environm Engn, Blacksburg, VA 24060 USA. RP Pruden, A (reprint author), Virginia Tech, Dept Civil & Environm Engn, Blacksburg, VA 24060 USA. EM apruden@vt.edu FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2013-68003-21256]; USDA NIFA [2014-05280] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-68003-21256 from the USDA National Institute of Food and Agriculture. Amy Pruden was also supported by USDA NIFA Grant no. 2014-05280. NR 130 TC 8 Z9 8 U1 16 U2 41 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 441 EP 453 DI 10.2134/jeq2015.07.0367 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900006 PM 27065390 ER PT J AU Durso, LM Wedin, DA Gilley, JE Miller, DN Marx, DB AF Durso, Lisa M. Wedin, David A. Gilley, John E. Miller, Daniel N. Marx, David B. TI Assessment of Selected Antibiotic Resistances in Ungrazed Native Nebraska Prairie Soils SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID HUMAN HEALTH; ANTIMICROBIAL RESISTANCE; TETRACYCLINE RESISTANCE; NATURAL ENVIRONMENTS; ESCHERICHIA-COLI; UNITED-STATES; FOOD ANIMALS; SWINE FARMS; GENES; RESISTOME AB The inherent spatial heterogeneity and complexity of antibiotic-resistant bacteria and antibiotic resistance (AR) genes in manure-affected soils makes it difficult to sort out resistance that can be attributed to human antibiotic use from resistance that occurs naturally in the soil. This study characterizes native Nebraska prairie soils that have not been affected by human or food-animal waste products to provide data on background levels of resistance in southeastern Nebraskan soils. Soil samples were collected from 20 sites enumerated on tetracycline and cefotaxime media; screened for tetracycline-, sulfonamide-, beta-lactamase-, and macrolide-resistance genes; and characterized for soil physical and chemical parameters. All prairies contained tetracycline- and cefotaxime-resistant bacteria, and 48% of isolates collected were resistant to two or more antibiotics. Most (98%) of the soil samples and all 20 prairies had at least one tetracycline gene. Most frequently detected were tet(D), tet(A) tet(O), tet(L), and tet(B). Sulfonamide genes, which are considered a marker of human or animal activity, were detected in 91% of the samples, despite the lack of human inputs at these sites. No correlations were found between either phenotypic or genotypic resistance and soil physical or chemical parameters. Heterogeneity was observed in AR within and between prairies. Therefore, multiple samples are necessary to overcome heterogeneity and to accurately assess AR. Conclusions regarding AR depend on the gene target measured. To determine the impacts of food-animal antibiotic use on resistance, it is essential that background and/or baseline levels be considered, and where appropriate subtracted out, when evaluating AR in agroecosystems. C1 [Durso, Lisa M.; Gilley, John E.; Miller, Daniel N.] Univ Nebraska, USDA ARS, AMRU, 121 Keim Hall,East Campus, Lincoln, NE 68583 USA. [Wedin, David A.] Univ Nebraska, Sch Nat Resources, 411 Hardin Hall, Lincoln, NE 68583 USA. [Marx, David B.] Univ Nebraska, Dept Stat, 340 Hardin Hall, Lincoln, NE 68583 USA. RP Durso, LM (reprint author), Univ Nebraska, USDA ARS, AMRU, 121 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM lisa.durso@ars.usda.gov OI Miller, Daniel/0000-0003-3476-487X FU National Program 212 Soil and Air Research Program FX The authors thank Jennifer McGhee, Jaime LaBrie, Amy Mantz, Angel Iverson, Katherine Herman, and Emily Hubl for laboratory and field support; Sara Murray, Ryan McGhee, Aaron Bereuter, Tyler Hoehn, and Tyler Goeschel for assistance in collecting samples; and Paul Koerner and Myron Coleman for additional technical support. We also extend our sincere thanks to the land owners and managers for maintaining the prairie sites and allowing us to collect samples. This work was funded by USDA-ARS National Program 214, Agricultural and Industrial Byproduct Utilization, now part of National Program 212 Soil and Air Research Program. NR 52 TC 2 Z9 2 U1 5 U2 18 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 454 EP 462 DI 10.2134/jeq2015.06.0280 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900007 PM 27065391 ER PT J AU Liu, CH Chuang, YH Li, H Teppen, BJ Boyd, SA Gonzalez, JM Johnston, CT Lehmann, J Zhang, W AF Liu, Cheng-Hua Chuang, Ya-Hui Li, Hui Teppen, Brian J. Boyd, Stephen A. Gonzalez, Javier M. Johnston, Cliff T. Lehmann, Johannes Zhang, Wei TI Sorption of Lincomycin by Manure-Derived Biochars from Water SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID AQUEOUS-SOLUTIONS; ACTIVATED CARBON; BLACK CARBON; SOILS; PHARMACEUTICALS; ADSORPTION; ANTIBIOTICS; REMOVAL; TETRACYCLINE; CONTAMINANTS AB The presence of antibiotics in agroecosystems raises concerns about the proliferation of antibiotic-resistant bacteria and adverse effects to human health. Soil amendment with biochars pyrolized from manures may be a win-win strategy for novel manure management and antibiotics abatement. In this study, lincomycin sorption by manure-derived biochars was examined using batch sorption experiments. Lincomycin sorption was characterized by two-stage kinetics with fast sorption reaching quasi-equilibrium in the first 2 d, followed by slow sorption over 180 d. The fast sorption was primarily attributed to surface adsorption, whereas the long-term slow sorption was controlled by slow diffusion of lincomycin into biochar pore structures. Two-day sorption experiments were performed to explore effects of biochar particle size, solid/water ratio, solution pH, and ionic strength. Lincomycin sorption to biochars was greater at solution pH 6.0 to 7.5 below the dissociation constant of lincomycin (7.6) than at pH 9.9 to 10.4 above its dissociation constant. The enhanced lincomycin sorption at lower pH likely resulted from electrostatic attraction between the positively charged lincomycin and the negatively charged biochar surfaces. This was corroborated by the observation that lincomycin sorption decreased with increasing ionic strength at lower pH (6.7) but remained constant at higher pH (10). The long-term lincomycin sequestration by biochars was largely due to pore diffusion plausibly independent of solution pH and ionic composition. Therefore, manure-derived biochars had lasting lincomycin sequestration capacity, implying that biochar soil amendment could significantly affect the distribution, transport, and bioavailability of lincomycin in agroecosystems. C1 [Liu, Cheng-Hua; Chuang, Ya-Hui; Li, Hui; Teppen, Brian J.; Boyd, Stephen A.; Zhang, Wei] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Liu, Cheng-Hua; Zhang, Wei] Michigan State Univ, Environm Sci & Policy Program, E Lansing, MI 48824 USA. [Gonzalez, Javier M.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Johnston, Cliff T.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Lehmann, Johannes] Cornell Univ, Soil & Crop Sci, Ithaca, NY 14853 USA. RP Zhang, W (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA.; Zhang, W (reprint author), Michigan State Univ, Environm Sci & Policy Program, E Lansing, MI 48824 USA. EM weizhang@msu.edu RI Liu, Cheng-Hua/C-3677-2014; Li, Hui/G-4055-2010; OI Li, Hui/0000-0003-3298-5265; Zhang, Wei/0000-0002-2937-1732 FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2013-67019-21377] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant No. 2013-67019-21377 from the USDA National Institute of Food and Agriculture. The views and opinions of the authors expressed herein do not necessarily reflect those of USDA and the authors' organizations. Mention of tradenames or commercial products does not constitute endorsement or recommendation for use. NR 45 TC 2 Z9 2 U1 18 U2 49 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 519 EP 527 DI 10.2134/jeq2015.06.0320 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900015 PM 27065399 ER PT J AU Franklin, AM Williams, CF Andrews, DM Woodward, EE Watson, JE AF Franklin, Alison M. Williams, Clinton F. Andrews, Danielle M. Woodward, Emily E. Watson, John E. TI Uptake of Three Antibiotics and an Antiepileptic Drug by Wheat Crops Spray Irrigated with Wastewater Treatment Plant Effluent SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PERSONAL CARE PRODUCTS; HUMAN PHARMACEUTICALS; SULFONAMIDE ANTIBIOTICS; VETERINARY ANTIBIOTICS; SORPTION-DESORPTION; MUNICIPAL BIOSOLIDS; AQUATIC ENVIRONMENT; SATIVA L.; SOIL; CARBAMAZEPINE AB With rising demands on water supplies necessitating water reuse, wastewater treatment plant (WWTP) effluent is often used to irrigate agricultural lands. Emerging contaminants, like pharmaceuticals and personal care products (PPCPs), are frequently found in effluent due to limited removal during WWTP processes. Concern has arisen about the environmental fate of PPCPs, especially regarding plant uptake. The aim of this study was to analyze uptake of sulfamethoxazole, trimethoprim, ofloxacin, and carbamazepine in wheat (Triticum aestivum L.) plants that were spray-irrigated with WWTP effluent. Wheat was collected before and during harvest, and plants were divided into grain and straw. Subsamples were rinsed with methanol to remove compounds adhering to surfaces. All plant tissues underwent liquid-solid extraction, solid-phase extraction cleanup, and liquid chromatography-tandem mass spectrometry analysis. Residues of each compound were present on most plant surfaces. Ofloxacin was found throughout the plant, with higher concentrations in the straw (10.2 +/- 7.05 ng g(-1)) and lower concentrations in the grain (2.28 +/- 0.89 ng g(-1)). Trimethoprim was found only on grain or straw surfaces, whereas carbamazepine and sulfamethoxazole were concentrated within the grain (1.88 +/- 2.11 and 0.64 +/- 0.37 ng g(-1), respectively). These findings demonstrate that PPCPs can be taken up into wheat plants and adhere to plant surfaces when WWTP effluent is spray-irrigated. The presence of PPCPs within and on the surfaces of plants used as food sources raises the question of potential health risks for humans and animals. C1 [Franklin, Alison M.; Andrews, Danielle M.; Woodward, Emily E.; Watson, John E.] Penn State Univ, Dept Ecosyst Sci & Management, 116 ASI Bldg, University Pk, PA 16802 USA. [Williams, Clinton F.] USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85238 USA. RP Franklin, AM (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, 116 ASI Bldg, University Pk, PA 16802 USA. EM amy125@psu.edu FU Pennsylvania State University's Office of Physical Plant and Regional Research Projects [W-2082, W3170] FX This study was partially funded by The Pennsylvania State University's Office of Physical Plant and Regional Research Projects W-2082 and W3170. The present work was partially developed within the framework of the Panta Rhei Research Initiative of the International Association of Hydrological Sciences (IAHS). The authors thank Allan Knopf at the US Arid Land Agricultural Research Center and Philip Smith at the Metabolomics Facility for technical assistance. NR 72 TC 1 Z9 1 U1 12 U2 32 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 546 EP 554 DI 10.2134/jeq2015.05.0257 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900018 PM 27065402 ER PT J AU Nordenholt, RM Goyne, KW Kremer, RJ Lin, CH Lerch, RN Veum, KS AF Nordenholt, Rebecca M. Goyne, Keith W. Kremer, Robert J. Lin, Chung-Ho Lerch, Robert N. Veum, Kristen S. TI Veterinary Antibiotic Effects on Atrazine Degradation and Soil Microorganisms SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID MICROBIAL-COMMUNITY STRUCTURE; CHROMATOGRAPHY-MASS SPECTROMETRY; MANURE-AMENDED SOIL; SP STRAIN ADP; ORGANIC AMENDMENTS; PSEUDOMONAS SP; ANIMAL MANURE; TRANSPORT PARAMETERS; HERBICIDE ATRAZINE; RESISTANCE GENES AB Veterinary antibiotics (VAs) in manure applied to agricultural lands may change agrichemical degradation by altering soil microbial community structure or function. The objectives of this study were to investigate the influence of two VAs, sulfamethazine (SMZ) and oxytetracycline (OTC), on atrazine (ATZ) degradation, soil microbial enzymatic activity, and phospholipid fatty acid (PLFA) markers. Sandy loam soil with and without 5% swine manure (w/w) was amended with 0 or 500 mg kg(-1) C-14 radiolabeled ATZ and with 0, 100, or 1000 mg kg-1 SMZ or OTC and incubated at 25 degrees C in the dark for 96 d. The half-life of ATZ was not significantly affected by VA treatment in the presence or absence of manure; however, the VAs significantly (p < 0.05) inhibited ATZ mineralization in soil without manure (25-50% reduction). Manure amendment decreased ATZ degradation by 22%, reduced ATZ mineralization by 50%, and increased the half-life of ATZ by >10 d. The VAs had limited adverse effects on the microbial enzymes beta-glucosidase and dehydrogenase in soils with and without manure. In contrast, manure application stimulated dehydrogenase activity and altered chlorinated ATZ metabolite profiles. Concentrations of PLFA markers were reduced by additions of ATZ, manure, OTC, and SMZ; adverse additive effects of combined treatments were noted for arbuscular mycorrhizal fungi and actinobacteria. In this work, the VAs did not influence persistence of the ATZ parent compound or chlorinated ATZ metabolite formation and degradation. However, reduced (CO2)-C-14 evolved from VA-treated soil suggests an inhibition to the degradation of other ATZ metabolites due to an altered soil microbial community structure. C1 [Nordenholt, Rebecca M.; Goyne, Keith W.; Kremer, Robert J.] Univ Missouri, Dept Soil Environm & Atmospher Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Lin, Chung-Ho] Univ Missouri, Ctr Agroforestry, Dept Forestry, 203 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Lerch, Robert N.; Veum, Kristen S.] USDA ARS, Cropping Syst & Water Qual Res Unit, 269 Agr Engn Bldg, Columbia, MO 65211 USA. RP Goyne, KW (reprint author), Univ Missouri, Dept Soil Environm & Atmospher Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM goynek@missouri.edu FU USDA National Institute of Food and Agriculture, Multi-state project [W2082, 231774]; University of Missouri Graduate School FX This work was supported by the USDA National Institute of Food and Agriculture, Multi-state project W2082, 231774 and the University of Missouri Graduate School. The authors thank Bettina Coggeshall, Amber Spohn, and Joe Absheer of the USDA-ARS and Claire Friedrichsen and Dr. Cammy Willett of the University of Missouri for assistance in the laboratory, and Cedar Creek Farms of Osage County, MO, for permission to acquire soil and manure from their property. NR 93 TC 1 Z9 1 U1 16 U2 32 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 565 EP 575 DI 10.2134/jeq2015.05.0235 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900020 PM 27065404 ER PT J AU Rothrock, MJ Hiett, KL Guard, JY Jackson, CR AF Rothrock, Michael J., Jr. Hiett, Kelli L. Guard, Jean Y. Jackson, Charlene R. TI Antibiotic Resistance Patterns of Major Zoonotic Pathogens from All-Natural, Antibiotic-Free, Pasture-Raised Broiler Flocks in the Southeastern United States SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ANTIMICROBIAL RESISTANCE; LISTERIA-MONOCYTOGENES; SALMONELLA-ENTERICA; CAMPYLOBACTER SPP.; ESCHERICHIA-COLI; CHICKEN PRODUCTS; WASTE-WATER; PREVALENCE; POULTRY; BACTERIA AB The use of antibiotics in agroecosystems has been implicated in the rise in antibiotic resistance (AR), which can affect environmental, animal, and human health. To determine the environmental impact of antibiotic use in agroecosystems, appropriate background levels of AR in agricultural environments in the absence of antibiotic application must be determined. Therefore, to determine background levels of AR in broiler production, four target microbes (Escherichia coli, Salmonella, Campylobacter, and Listeria) were isolated from 15 all-natural, antibiotic-free, pasture-raised broiler flocks from six farms within the southeastern United States. The AR profiles of these isolates were characterized using the CDC National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS), and these resistance patterns were compared across target microbes and farms and throughout the life cycle of the flocks along the farm-to-fork continuum. Antibiotic resistances were most prevalent in Listeria and Salmonella and least prevalent in Campylobacter. Although E. coli and Salmonella were isolated from the same farms and characterized using the same NARMS plates, they exhibited distinct AR profiles, with Salmonella demonstrating clear farm-specific resistance patterns. Multidrug resistance rates (three or more antibiotics), in order of prevalence, were Listeria (63.9%), Salmonella (36.0%), E. coli (12.7%), and Campylobacter (1.4%). The results of this study demonstrate the variability in background AR among major food safety-related microbes, even when isolated from similar production and processing samples from the same farms, and indicate the need for the proper design of future broiler production studies to account for this highly dynamic background AR. C1 [Rothrock, Michael J., Jr.; Guard, Jean Y.] USDA ARS, US Natl Poultry Res Ctr, Egg Safety & Qual Res Unit, 950 Coll Stn Rd, Athens, GA 30606 USA. [Hiett, Kelli L.] USDA ARS, US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Res Unit, 950 Coll Stn Rd, Athens, GA 30606 USA. [Jackson, Charlene R.] USDA ARS, US Natl Poultry Res Ctr, Bacteriol Epidemiol & Antibiot Resistance Res Uni, 950 Coll Stn Rd, Athens, GA 30606 USA. RP Rothrock, MJ (reprint author), USDA ARS, US Natl Poultry Res Ctr, Egg Safety & Qual Res Unit, 950 Coll Stn Rd, Athens, GA 30606 USA. EM michael.rothrock@ars.usda.gov FU Agricultural Research Service, USDA CRIS [6040-32000-007-00, 6612-32000-059-00] FX The authors thank Katelyn Griffin, Ariella Perry, Hellen Noh, Sarah Khorramzadeh, and Manju Amin for assistance in sample acquisition and pathogen isolation and Laura Lee Rutherford, Cheryl Gresham-Pearson, and Latoya Wiggins for performing the NARMS panels. These investigations were supported by the Agricultural Research Service, USDA CRIS Projects "Genetic Analysis of Poultry-Associated Salmonella enterica to Identify and Characterize Properties and Markers Associated with Egg-Borne Transmission of Illness" #6040-32000-007-00 and "Molecular Approaches for the Characterization of Foodborne Pathogens in Poultry" #6612-32000-059-00. NR 48 TC 1 Z9 1 U1 5 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 593 EP 603 DI 10.2134/jeq2015.07.0366 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900022 PM 27065406 ER PT J AU Whitehead, TR Cotta, MA AF Whitehead, Terence R. Cotta, Michael A. TI Examination of the Aerobic Microflora of Swine Feces and Stored Swine Manure SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID TETRACYCLINE RESISTANCE GENES; STORAGE PITS; SP-NOV.; UNITED-STATES; BACTERIA; DIVERSITY; IDENTIFICATION; ANTIMICROBIALS; VALIDATION; PRIMERS AB Understanding antibiotic resistance in agricultural ecosystems is critical for determining the effects of subtherapeutic and therapeutic uses of antibiotics for domestic animals. This study was conducted to ascertain the relative levels of antibiotic resistance in the aerobic bacterial population to tetracycline, tylosin, and erythromycin. Swine feces and manure samples were plated onto various agar media with and without antibiotics and incubated at 37 degrees C. Colonies were counted daily. Randomly selected colonies were isolated and characterized by 16S rRNA sequence analyses and additional antibiotic resistance and biochemical analyses. Colonies were recovered at levels of 105 to 107 CFU mL(-1) for swine slurry and 106 to 108 CFU g(-1) swine feces, approximately 100-fold lower than numbers obtained under anaerobic conditions. Addition of antibiotics to the media resulted in counts that were 60 to 80% of those in control media without added antibiotics. Polymerase chain reaction analyses for antibiotic resistance genes demonstrated the presence of a number of different tet resistance genes from the isolates. The recoverable aerobic microflora of swine feces and manure contain high percentages of antibiotic-resistant bacteria, which include both known and novel genera and species, and a variety of antibiotic resistance genes. Further analyses of these and additional isolates should provide additional information on these organisms as potential reservoirs of antibiotic resistance genes in these ecosystems. C1 [Whitehead, Terence R.; Cotta, Michael A.] USDA ARS, Bioenergy Res Unit, Natl Agr Res Ctr, 1815 N Univ St, Peoria, IL 61604 USA. RP Whitehead, TR (reprint author), USDA ARS, Bioenergy Res Unit, Natl Agr Res Ctr, 1815 N Univ St, Peoria, IL 61604 USA. EM Terry.Whitehead@ars.usda.gov NR 30 TC 2 Z9 2 U1 3 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 604 EP 608 DI 10.2134/jeq2015.05.0248 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900023 PM 27065407 ER PT J AU Zwonitzer, MR Soupir, ML Jarboe, LR Smith, DR AF Zwonitzer, Martha R. Soupir, Michelle L. Jarboe, Laura R. Smith, Douglas R. TI Quantifying Attachment and Antibiotic Resistance of Escherichia coli from Conventional and Organic Swine Manure SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SATURATED POROUS-MEDIA; ANTIMICROBIAL RESISTANCE; BIOFILM FORMATION; URINARY-TRACT; TRANSPORT BEHAVIOR; INDICATOR BACTERIA; ANTIPORT PROTEIN; CELL PROPERTIES; SURFACE-WATER; P-FIMBRIAE AB Broad-spectrum antibiotics are often administered to swine, contributing to the occurrence of antibiotic-resistant bacteria in their manure. During land application, the bacteria in swine manure preferentially attach to particles in the soil, affecting their transport in overland flow. However, a quantitative understanding of these attachment mechanisms is lacking, and their relationship to antibiotic resistance is unknown. The objective of this study is to examine the relationships between antibiotic resistance and attachment to very fine silica sand in Escherichia coli collected from swine manure. A total of 556 isolates were collected from six farms, two organic and four conventional (antibiotics fed prophylactically). Antibiotic resistance was quantified using 13 antibiotics at three minimum inhibitory concentrations: resistant, intermediate, and susceptible. Of the 556 isolates used in the antibiotic resistance assays, 491 were subjected to an attachment assay. Results show that E. coli isolates from conventional systems were significantly more resistant to amoxicillin, ampicillin, chlortetracycline, erythromycin, kanamycin, neomycin, streptomycin, tetracycline, and tylosin (P < 0.001). Results also indicate that E. coli isolated from conventional systems attached to very fine silica sand at significantly higher levels than those from organic systems (P < 0.001). Statistical analysis showed that a significant relationship did not exist between antibiotic resistance levels and attachment in E. coli from conventional systems but did for organic systems (P < 0.001). Better quantification of these relationships is critical to understanding the behavior of E. coli in the environment and preventing exposure of human populations to antibiotic-resistant bacteria. C1 [Zwonitzer, Martha R.] Texas A&M AgriLife Res, 1102 East FM 1294, Lubbock, TX 79403 USA. [Zwonitzer, Martha R.; Soupir, Michelle L.] Iowa State Univ, Dept Agr & Biosyst Engn, 3358 Elings Hall, Ames, IA 50011 USA. [Jarboe, Laura R.] Iowa State Univ, Dept Chem & Biol Engn, 2114 Sweeney Hall, Ames, IA 50011 USA. [Smith, Douglas R.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. RP Zwonitzer, MR (reprint author), Texas A&M AgriLife Res, 1102 East FM 1294, Lubbock, TX 79403 USA.; Zwonitzer, MR (reprint author), Iowa State Univ, Dept Agr & Biosyst Engn, 3358 Elings Hall, Ames, IA 50011 USA. EM martha.zwonitzer@ag.tamu.edu FU Iowa State University FX Funding for this work was provided by Iowa State University. The authors thank Bridget Huss, Molly Zwonitzer, Pagie Dugal Arve, and David Westhoff for their assistance with sample collection and analysis; ISU research farms and other private farms for providing access to manure samples; and Elaine Eisenbeisz, Heather Torres, and Heather Crutcher at Omega Statistics for help in analyzing these datasets. NR 77 TC 1 Z9 1 U1 10 U2 22 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 609 EP 617 DI 10.2134/jeq2015.05.0245 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900024 PM 27065408 ER PT J AU Spiehs, MJ Brown-Brandl, TM Parker, DB Miller, DN Berry, ED Wells, JE AF Spiehs, Mindy J. Brown-Brandl, Tami M. Parker, David B. Miller, Daniel N. Berry, Elaine D. Wells, James E. TI Ammonia, Total Reduced Sulfides, and Greenhouse Gases of Pine Chip and Corn Stover Bedding Packs SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID BEDDED MANURE PACKS; DEEP-LITTER SYSTEMS; NITROUS-OXIDE; METHANE EMISSIONS; CHAMBER; WASTES; ADM1 AB Bedding materials may affect air quality in livestock facilities. Our objective in this study was to compare headspace concentrations of ammonia (NH3), total reduced sulfides (TRS), carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) when pine wood chips (Pinus spp.) and corn stover (Zea mays L.) were mixed in various ratios (0, 10, 20, 30, 40, 60, 80, and 100% pine chips) and used as bedding with manure. Air samples were collected from the headspace of laboratory-scaled bedded manure packs weekly for 42 d. Ammonia concentrations were highest for bedded packs containing 0, 10, and 20% pine chips (equivalent to 501.7, 502.3, and 502.3 mg m(-3), respectively) in the bedding mixture and were lowest when at least 80% pine chips were used as bedding (447.3 and 431.0 mg m(-3), respectively for 80 and 100% pine chip bedding). The highest NH3 concentrations were observed at Day 28. The highest concentration of TRS was observed when 100% pine chips were used as bedding (11.4 mu g m(-3)), with high concentrations occurring between Days 7 and 14, and again at Day 35. Greenhouse gases were largely unaffected by bedding material but CH4 and CO2 concentrations increased as the bedded packs aged and N2O concentrations were highly variable throughout the incubation. We conclude that a mixture of bedding material that contains 30 to 40% pine chips may be the ideal combination to reduce both NH3 and TRS emissions. All gas concentrations increased as the bedded packs aged, suggesting that frequent cleaning of facilities would improve air quality in the barn, regardless of bedding materials used. C1 [Spiehs, Mindy J.; Brown-Brandl, Tami M.; Berry, Elaine D.; Wells, James E.] USDA ARS, Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Parker, David B.] USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. [Miller, Daniel N.] USDA, Agroecosyst Management Res Unit, Lincoln, NE USA. RP Spiehs, MJ (reprint author), USDA ARS, Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM mindy.spiehs@ars.usda.gov OI Miller, Daniel/0000-0003-3476-487X NR 39 TC 1 Z9 1 U1 2 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 630 EP 637 DI 10.2134/jeq2015.09.0466 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900026 PM 27065410 ER PT J AU Partyka, ML Bond, RF Chase, JA Kiger, L Atwill, ER AF Partyka, Melissa L. Bond, Ronald F. Chase, Jennifer A. Kiger, Luana Atwill, Edward R. TI Multistate Evaluation of Microbial Water and Sediment Quality from Agricultural Recovery Basins SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ESCHERICHIA-COLI O157/H7; INDICATOR ORGANISMS; IRRIGATION WATER; ANTIBIOTIC-RESISTANCE; SALMONELLA; SURVIVAL; PATHOGENS; PRODUCE; STREAM; RIVER AB Agricultural recovery basins are an important conservation practice designed to provide temporary storage of sediment and water on farms before low-volume discharge. However, food safety concerns have been raised regarding redistribution of captured sediment and water to fields used for human food production. The purpose of this study was to examine the potential microbiological risk that recovery basins may contribute to nearby produce fields and to evaluate characteristics that may influence or mitigate those risks. Water and sediment samples were collected from participating farms in three states and evaluated for bacterial indicators and pathogens over several months. Overall, 45% (n = 48) of water samples and less than 15% (n = 13) of sediment samples were positive for Salmonella spp. In water samples, the occurrence of Salmonella was positively associated with the use of surface water as a source of irrigation compared with groundwater as well as log-scale increases in Escherichia coli concentration. In sediment samples, Salmonella was associated with basin location (region) and basin fill levels. Sediment exposed to drying during dewatering had lower concentrations of indicator E. coli and a lower proportion of Salmonella positives than submerged sediment from the same pond. Surrounding landscape characteristics, including vegetative coverage, proximity to livestock operations, and evidence of wildlife, were not correlated with pathogen occurrence in either sediment or water samples, suggesting that although habitat surrounding ponds may be an attractant to wildlife, those features may not contribute to increased pathogen occurrence in agricultural recovery basins. C1 [Partyka, Melissa L.; Bond, Ronald F.; Chase, Jennifer A.] Univ Calif Davis, Western Inst Food Safety & Secur, 1089 Vet Med Dr, Davis, CA 95616 USA. [Kiger, Luana] USDA, NRCS, 430 G St 4164, Davis, CA 95616 USA. [Atwill, Edward R.] Univ Calif Davis, Western Ctr Food Safety, One Shields Ave, Davis, CA 95616 USA. RP Partyka, ML (reprint author), Univ Calif Davis, Western Inst Food Safety & Secur, 1089 Vet Med Dr, Davis, CA 95616 USA. EM mlpartyka@ucdavis.edu FU USDA-NRCS [65-9104-0-816 (817)]; USFDA [U01-003-572]; Western Center for Food Safety FX This research was supported by grants through the USDA-NRCS, Agreement # 65-9104-0-816 (817) and the USFDA, contract U01-003-572 with the Western Center for Food Safety. The authors thank NRCS staff in California (Truong Ha and Rebecca Challender), Florida (Eric Theurer, Steve Boetger, Anthony Harvey, and Greg Hendricks), and Mississippi (Al Garner and Wesley Kerr) for their assistance in establishing grower relationships; Dawn M. Rebarchik-University of Southern Mississippi, Gulf Coast Research Laboratory for the use of laboratory space and professional guidance; and growers in California, Florida, and Mississippi who participated in this project. NR 39 TC 1 Z9 1 U1 5 U2 16 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 657 EP 665 DI 10.2134/jeq2015.06.0323 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900029 PM 27065413 ER PT J AU Poudyal, S Zheljazkov, VD Cantrell, CL Kelleners, T AF Poudyal, Shital Zheljazkov, Valtcho D. Cantrell, Charles L. Kelleners, Thijs TI Coal-Bed Methane Water Effects on Dill and Its Essential Oils SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PEPPERMINT; SOIL; PLANT; HERB; CU AB Pumping water from coal seams decreases the pressure in the seam and in turn releases trapped methane; this is the most common and economic method of methane extraction. The water that is pumped out is known as "coal-bed methane water" (CBMW), which is high in sodium and other salts. In the past 25 yr, the United States has seen a 16-fold increase in the production of coal bed methane gas, and trillions of cubic meters are yet to be extracted. There is no sustainable disposal method for CBMW, and there are very few studies investigating the effects of this water on plants and their secondary metabolites and on soil properties. This study was conducted to determine the effects of CBMW on soil chemical properties and on the biomass and essential oil yield and composition of dill (Anethum graveolens L.). This crop was grown in a greenhouse and was subjected to different levels of CBMW treatment: tap water only; 25% CBMW, 75% tap water; 50% CBMW, 50% tap water; 75% CBMW, 25% tap water; and 100% CBMW. The major dill oil constituents, limonene and a-phellandrene, were not affected by the treatments; however, the concentration of dill ether increased with increasing CBMW levels, whereas the concentration of carvone decreased. In soil, sodium level significantly increased with increasing level of treatment, but pH and cation exchange capacity were not much affected. Coal bed methane water could be used for irrigation of dill for one growing season, but longer-term studies may be needed to clarify the long-term effects on soil and plant. C1 [Poudyal, Shital] Univ Wyoming, Dept Plant Sci, 1000 Univ Ave, Laramie, WY 82071 USA. [Zheljazkov, Valtcho D.] Oregon State Univ, Columbia Basin Agr Res Ctr, 48037 Tubbs Ranch Rd,POB 370, Pendleton, OR 97801 USA. [Cantrell, Charles L.] USDA ARS, NPURU, University, MS 38677 USA. [Kelleners, Thijs] Univ Wyoming, Ecosyst Sci & Manag Dept, 1000 Univ Ave, Laramie, WY 82071 USA. RP Zheljazkov, VD (reprint author), Oregon State Univ, Columbia Basin Agr Res Ctr, 48037 Tubbs Ranch Rd,POB 370, Pendleton, OR 97801 USA. EM Valtcho.Jeliazkov@oregonstate.edu FU University of Wyoming School of Energy FX This research was supported by the University of Wyoming School of Energy funds awarded to Dr. Valtcho D. Zheljazkov (Jeliazkov). The authors thank Derek Lowe of BeneTerra LLC, in Sheridan, WY, for providing access to coal-bed methane water and Dan Smith and Jeremiah Vardiman, farm manager and research associate, respectively, at the University of Wyoming's Sheridan Research and Extension Center, Sheridan, who helped with the field trial management. NR 41 TC 0 Z9 0 U1 2 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 728 EP 733 DI 10.2134/jeq2015.05.0215 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900037 PM 27065421 ER PT J AU Fenstermacher, DE Rabenhorst, MC Lang, MW McCarty, GW Needelman, BA AF Fenstermacher, D. E. Rabenhorst, M. C. Lang, M. W. McCarty, G. W. Needelman, B. A. TI Carbon in Natural, Cultivated, and Restored Depressional Wetlands in the Mid-Atlantic Coastal Plain SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SOIL CARBON; CAROLINA BAYS; UNITED-STATES; MARYLAND; USA AB Aerial extent of wetland ecosystems has decreased dramatically since precolonial times due to the conversion of these areas for human use. Wetlands provide various ecosystem services, and conservation efforts are being made to restore wetlands and their functions, including soil carbon storage. This Mid-Atlantic Regional USDA Wetland Conservation Effects Assessment Project study was conducted to evaluate the effects and effectiveness of wetland conservation practices along the Mid-Atlantic Coastal Plain. This study examined 48 wetland sites in Delaware, Maryland, Virginia, and North Carolina under natural, prior converted cropland, and 5- to 10-yr post wetland restoration states. The North Carolina sites mainly contained soils dominated by organic soil materials and therefore were analyzed separately from the rest of the sites, which primarily contained mineral soils. Soil samples were collected using the bulk density core method by horizon to a depth of 1 m and were analyzed for percent carbon. The natural wetlands were found to have significantly greater carbon stocks (21.5 +/- 5.2 kg C m(-2)) than prior converted croplands (7.95 +/- 1.93 kg C m(-2); p < 0.01) and restored wetlands (4.82 +/- 1.13 kg C m(-2); p < 0.001). The restored and prior converted sites did not differ significantly, possibly the result of the methods used to restore the wetlands, and the relatively young age of the restored sites. Wetlands were either restored by plugging drainage structures, with minimal surface disturbance, or by scraping the surface (i.e., excavation) to increase hydroperiod. Sites restored with the scraping technique had significantly lower carbon stocks (2.70 +/- 0.38 kg C m(-2)) than those restored by passive techniques (6.06 +/- 1.50 kg C m(-2); p = 0.09). Therefore, techniques that involve excavation and scraping to restore hydrology appear to negatively affect C storage. C1 [Fenstermacher, D. E.; Rabenhorst, M. C.; Needelman, B. A.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Lang, M. W.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [McCarty, G. W.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Rabenhorst, MC (reprint author), Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. EM mrabenho@umd.edu FU Wetland Component of the USDA-NRCS National Conservation Effects Assessment Project FX This research was supported by the Wetland Component of the USDA-NRCS National Conservation Effects Assessment Project. NR 40 TC 0 Z9 0 U1 12 U2 27 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2016 VL 45 IS 2 BP 743 EP 750 DI 10.2134/jeq2015.04.0186 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DG1AR UT WOS:000371797900039 PM 27065423 ER PT J AU Solval, KM Bankston, JD Bechtel, PJ Sathivel, S AF Solval, Kevin Mis Bankston, J. David Bechtel, Peter J. Sathivel, Subramaniam TI Physicochemical Properties of Microencapsulated omega-3 Salmon Oil with Egg White Powder SO JOURNAL OF FOOD SCIENCE LA English DT Article DE egg white powder; microencapsulation; salmon oil; spray drying ID FISH-OIL; OXIDATIVE STABILITY; LIPID OXIDATION; XANTHAN GUM; SPRAY; FOOD; PROTEIN; STORAGE; ACID; TECHNOLOGY AB The objective of this study was to produce microencapsulated omega(omega)-3 fatty acids (PUFAs) fortified egg white (EW) powders and to characterize their nutritional and physical properties. Stable emulsions (E-SO-EW) containing 3.43 (g/100 g) salmon oil (SO), 56.21 (g/100 g) EW, and 40.36 (g/100 g) water and a control (E-EW) containing EW and water were prepared. E-SO-EW and E-EW were separately spray dried at 130, 140, and 150 degrees C inlet air temperatures. This resulted in 3 microencapsulated SO fortified EW powders (SO-EW), and 3 dried EW powders (DEW). The powders were analyzed for microencapsulation efficiency (ME), color, fatty acids methyl esters, protein, fat, moisture, ash, amino acids, minerals, microstructure, and particle size. The EPA and DHA content of SO and the ME of the powders were not affected by the inlet air temperature. The crude protein content of SO-EW powders was approximately 24 (g/100 g) lower than dried EW powders. Leucine was the most abundant essential amino acid found in all the powders. Most of the powders' median particle size ranged from 15 to 30 mu m. The study demonstrated that microencapsulated omega-3 salmon oil with high quality EW protein can be produced by spray drying. C1 [Solval, Kevin Mis; Sathivel, Subramaniam] Louisiana State Univ, Ctr Agr, Dept Biol & Agr Engn, Baton Rouge, LA 70803 USA. [Bechtel, Peter J.] Univ Alaska Fairbanks, USDA ARS, Subarct Res Unit, Kodiak, AK 99615 USA. [Bankston, J. David; Sathivel, Subramaniam] Louisiana State Univ, Ctr Agr, Sch Nutr & Food Sci, Baton Rouge, LA 70803 USA. RP Sathivel, S (reprint author), Louisiana State Univ, Ctr Agr, Dept Biol & Agr Engn, Baton Rouge, LA 70803 USA.; Sathivel, S (reprint author), Louisiana State Univ, Ctr Agr, Sch Nutr & Food Sci, Baton Rouge, LA 70803 USA. EM ssathivel@agcenter.lsu.edu NR 61 TC 1 Z9 1 U1 7 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2016 VL 81 IS 3 BP E600 EP E609 DI 10.1111/1750-3841.13228 PG 10 WC Food Science & Technology SC Food Science & Technology GA DG5VW UT WOS:000372148400007 ER PT J AU Geveke, DJ Gurtler, JB Jones, DR Bigley, ABW AF Geveke, David J. Gurtler, Joshua B. Jones, Deana R. Bigley, Andrew B. W. TI Inactivation of Salmonella in Shell Eggs by Hot Water Immersion and Its Effect on Quality SO JOURNAL OF FOOD SCIENCE LA English DT Article DE egg; food processing; food quality; pasteurization; Salmonella ID THERMAL-RESISTANCE; INTACT EGGS; HEAT; PASTEURIZATION; ENTERITIDIS; STRENGTH; STRAINS; ALBUMIN; SPP. AB Thermal inactivation kinetics of heat resistant strains of Salmonella Enteritidis in shell eggs processed by hot water immersion were determined and the effects of the processing on egg quality were evaluated. Shell eggs were inoculated with a composite of heat resistant Salmonella Enteritidis (SE) strains PT8 C405, 2 (FSIS #OB030832), and 6 (FSIS #OB040159). Eggs were immersed in a circulating hot water bath for various times and temperatures. Come-up time of the coldest location within the egg was 21 min. SE was reduced by 4.5 log at both hot water immersion treatments of 56.7 C for 60 min and 55.6 degrees C for 100 min. Decimal reduction times (D-values) at 54.4, 55.6, and 56.7 degrees C were 51.8, 14.6, and 9.33 min, respectively. The z-value was 3.07 degrees C. Following treatments that resulted in a 4.5 log reduction (56.7 degrees C/60 min and 55.6 degrees C/100 min), the surviving population of SE remained static during 4 wk of refrigerated storage. After processing under conditions resulting in 4.5 log reductions, the Haugh unit and albumen height significantly increased (P < 0.01) and yolk index significantly decreased (P < 0.05). The shell dynamic stiffness significantly increased (P < 0.05), while static compression shell strength showed no significant difference (P < 0.05). Vitelline membrane strength significantly increased (P < 0.05); although, no significant difference (P < 0.05) was observed in vitelline membrane elasticity. In summary, the hot water immersion process inactivated heat resistant SE in shell eggs by 4.5 log, but also significantly affected several egg quality characteristics. C1 [Geveke, David J.; Gurtler, Joshua B.; Bigley, Andrew B. W.] USDA, Eastern Reg Res Ctr, Agr Res Serv, Food Safety & Intervent Technol Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Jones, Deana R.] USDA, Agr Res Serv, US Natl Poultry Res Ctr, Egg Safety & Qual Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Geveke, DJ (reprint author), USDA, Eastern Reg Res Ctr, Agr Res Serv, Food Safety & Intervent Technol Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.geveke@ars.usda.gov FU USDA-ARS, Wyndmoor, PA FX The authors thank Ms. R. Bailey and Messrs. J. Charlton and O. Ukuku (all of the USDA-ARS, Wyndmoor, PA) and Ms. P. Mason (of the USDA-ARS, Athens, GA) for technical support. The authors thank Mr. J. Sites for photography. The Salmonella Enteritidis (SE) PT8 strain C405 was provided by Dr. K. Lampel (of the FDA-CFSAN, Laurel MD). Two SE strains, 2 (FSIS #OB030832) and 6 (FSIS #OB040159), were provided by Dr. W. Cray (of the USDA-FSIS, Athens, GA). The authors thank Dr. A. Singh, (an ORISE postdoctoral researcher sponsored by USDA-ARS, Wyndmoor, PA) for his thoughtful review of this manuscript. NR 32 TC 1 Z9 1 U1 4 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2016 VL 81 IS 3 BP M709 EP M714 DI 10.1111/1750-3841.13233 PG 6 WC Food Science & Technology SC Food Science & Technology GA DG5VW UT WOS:000372148400024 PM 26878421 ER PT J AU Huang, LH Vinyard, BT AF Huang, Lihan Vinyard, Bryan T. TI Direct Dynamic Kinetic Analysis and Computer Simulation of Growth of Clostridium perfringens in Cooked Turkey during Cooling SO JOURNAL OF FOOD SCIENCE LA English DT Article DE bootstrap; C. perfringens; dynamic modeling; Monte Carlo analysis; numerical analysis; optimization ID PREDICTIVE MICROBIOLOGY; CONFIDENCE-INTERVALS; FOODBORNE DISEASE; UNITED-STATES; BEEF; MODEL; EPIDEMIOLOGY; TEMPERATURES; PARAMETERS; OUTBREAKS AB This research applied a new 1-step methodology to directly construct a tertiary model that describes the growth of Clostridium perfringens in cooked turkey meat under dynamically cooling conditions. The kinetic parameters of the growth models were determined by numerical analysis and optimization using multiple dynamic growth curves. The models and kinetic parameters were validated using independent growth curves obtained under various cooling conditions. The results showed that the residual errors (epsilon) of the predictions followed a Laplace distribution that is symmetric with respect to epsilon = 0. For residual errors, 90.6% are within +/- 0.5 Log CFU/g and 73.4% are +/- 0.25 Log CFU/g for all growth curves used for validation. For relative growth <1.0 Log CFU/g, 88.9% of the residual errors are within +/- 0.5 Log CFU/g, and 63.0% are within +/- 0.25 Log CFU/g. For relative growth of <2.0 Log CFU/g, 92.7% of the residual errors are within +/- 0.5 Log CFU/g, and 70.3% are within +/- 0.25 Log CFU/g. The scale and distribution of residual errors clearly suggests that the models and estimated kinetic parameters are reasonably accurate in predicting the growth of C. perfringens. Monte Carlo simulation was used to estimate the probabilities of >1.0 and 2.0 Log CFU/g relative growth of C. perfringens in the final products at the end of cooling. This probabilistic process analysis approach provides a new alternative for estimating and managing the risk of a product and can help the food industry and regulatory agencies assess the safety of cooked meat in the event of cooling deviation. C1 [Huang, Lihan] USDA, Agr Res Serv, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Vinyard, Bryan T.] USDA, Agr Res Serv, Northeast Area, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Huang, LH (reprint author), USDA, Agr Res Serv, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov FU USDA/NIFA grant [2011-68003-20096] FX The author would like to thank Dr. Changcheng Li of Fujian Agriculture and Forestry Univ. of conducting experiments, and Mr. Harter Barry of USDA ARS for technical assistance. This project is partially supported by a USDA/NIFA grant (2011-68003-20096). NR 31 TC 2 Z9 2 U1 4 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAR PY 2016 VL 81 IS 3 BP M692 EP M701 DI 10.1111/1750-3841.13202 PG 10 WC Food Science & Technology SC Food Science & Technology GA DG5VW UT WOS:000372148400022 PM 26801359 ER PT J AU Frey, GE Chamberlain, JL AF Frey, Gregory E. Chamberlain, James L. TI Collection of Nontimber Forest Products from State Forests in the US South SO JOURNAL OF FORESTRY LA English DT Article DE nontimber forest products; public lands AB Little is known about the harvest of nontimber forest products (NTFPs) in state forests of the US South. We asked the state forestry agencies in all 13 southern states about the products harvested and the policies regulating harvest, as well as evidence of illegal harvest and the effects of harvest on biodiversity. Of the 12 southern states that have state forests, 7 allow some harvest of NTFPs and 6 of those have evidence of illegal harvesting. The most common products cited were pine straw, pine cones, and live plants used for transplants into nurseries and landscaping. Only two states had enough data on the impacts of harvest to say that there is no negative effect on biodiversity. C1 [Frey, Gregory E.; Chamberlain, James L.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC USA. RP Frey, GE; Chamberlain, JL (reprint author), US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC USA. EM gregoryefrey@fs.fed.us; jchamberlain@fs.fed.us OI Frey, Gregory/0000-0003-0538-0199 NR 13 TC 0 Z9 0 U1 0 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2016 VL 114 IS 2 BP 85 EP 88 DI 10.5849/jof.15-043 PG 4 WC Forestry SC Forestry GA DG4MS UT WOS:000372046900002 ER PT J AU Dwivedi, P Jagadish, A Schelhas, J AF Dwivedi, Puneet Jagadish, Arundhati Schelhas, John TI Perceptions of Stakeholder Groups about the Participation of African American Family Forest Landowners in Federal Landowner Assistance Programs SO JOURNAL OF FORESTRY LA English DT Article DE African American family forest landowner; federal landowner assistance programs; perceptions; stakeholder groups; southern United States ID INCENTIVE PROGRAMS; OWNERS; RACE AB This study examines perceptions of three stakeholder groups (African American Family Forest Landowner, Government Agency, and Nonprofit) regarding federal landowner assistance programs in the southern United States by combining a SWOT (strength, weakness, opportunity, and threat) analysis with the AHP (analytical hierarchy process). Factors with the highest priority values were professional advice, lack of staff members, forestland retention, and heirs' property under the SWOT categories of strength, weakness, opportunity, and threat across stakeholder groups, respectively. The guidelines for existing federal landowner assistance programs do not match the needs of African American family forest landowners, especially the mandatory requirements of clear land titles and initial upfront payments. Policy changes coupled with a more targeted and personal outreach approach focusing on capacity building of African American family forest landowners is needed to increase their participation in federal landowner assistance programs. C1 [Dwivedi, Puneet] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Jagadish, Arundhati] Univ Georgia, Athens, GA 30602 USA. [Schelhas, John] US Forest Serv, USDA, Southern Res Stn, Washington, DC 20525 USA. RP Dwivedi, P (reprint author), Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA.; Jagadish, A (reprint author), Univ Georgia, Athens, GA 30602 USA.; Schelhas, J (reprint author), US Forest Serv, USDA, Southern Res Stn, Washington, DC 20525 USA. EM puneetd@uga.edu; jagadish.arundhati@uga.edu; jschelhas@fs.fed.us FU US Endowment for Forestry and Communities; USDA Forest Service, Southern Research Station FX This work was supported by US Endowment for Forestry and Communities and USDA Forest Service, Southern Research Station. NR 26 TC 1 Z9 1 U1 0 U2 3 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2016 VL 114 IS 2 BP 89 EP 96 DI 10.5849/jof.14-152 PG 8 WC Forestry SC Forestry GA DG4MS UT WOS:000372046900003 ER PT J AU Bussey, J Davenport, MA Emery, MR Carroll, C AF Bussey, John Davenport, Mae A. Emery, Marla R. Carroll, Clint TI "A Lot of It Comes from the Heart": The Nature and Integration of Ecological Knowledge in Tribal and Nontribal Forest Management SO JOURNAL OF FORESTRY LA English DT Article DE traditional ecological knowledge; western scientific ecological knowledge; forest management; comanagement; adaptation ID INDIGENOUS KNOWLEDGE; CONSERVATION; CANADA AB This article explores the generation, transmission, and nature of ecological knowledge used by tribal and nontribal natural resource management agency personnel who collectively manage a 666,542-acre forest in northern Minnesota. Using key informant interviews and an adapted grounded theory analysis, we documented the forms of knowledge participants expressed in their descriptions of the forest and forest management, including traditional and western scientific ecological knowledge. We found that study participants across agencies use multiple forms of knowledge, that this knowledge is generated and transferred in distinct ways, and that participants acknowledge several challenges and opportunities to integration of traditional and western scientific knowledge in forest management. Overall, ecological knowledge expressed by study participants revealed multiple ways of knowing the forest. Knowledge varied most distinctly in the influence of cultural identity and spiritual or metaphysical connections to the forest on knowledge generation, transmission, and content. Formalizing existing informal knowledge integration efforts with attention to power structures, institutional culture, and knowledge application is recommended. C1 [Bussey, John] YMCA St Croix, Hudson, WI 54016 USA. [Davenport, Mae A.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Emery, Marla R.] US Forest Serv, USDA, Washington, DC 20525 USA. [Carroll, Clint] Univ Minnesota, Minneapolis, MN 55455 USA. [Carroll, Clint] Univ Colorado Boulder, Boulder, CO 80309 USA. RP Bussey, J (reprint author), YMCA St Croix, Hudson, WI 54016 USA.; Davenport, MA (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN USA.; Emery, MR (reprint author), US Forest Serv, USDA, Washington, DC 20525 USA.; Carroll, C (reprint author), Univ Minnesota, Minneapolis, MN 55455 USA.; Carroll, C (reprint author), Univ Colorado Boulder, Boulder, CO 80309 USA. EM john.bussey@ymcatwincities.org; mdaven@umn.edu; memery@fs.fed.us; clint.carroll@colorado.edu FU USDA Forest Service, Northern Research Station; USDA National Institute of Food and Agriculture, Hatch Project [229912] FX This project was supported by funding from the USDA Forest Service, Northern Research Station and the USDA National Institute of Food and Agriculture, Hatch Project 229912. We thank the staff and study participants with the Leech Lake Band of Ojibwe Division of Resource Management and the Chippewa National Forest for their collaboration and support. We also thank Shirley Nordrum, Extension Educator with University ofMinnesota (UMN) Extension at the Leech Lake Tribal College, for her guidance in study design and insight on intercultural communications. We thank Amanda Sames for her assistance with data collection and analysis. Finally, we extend gratitude to Kristen Nelson, UMN, Pamela lakes, USDA Forest Service (retired), and the anonymous reviewers for their review and recommendations on this article. NR 37 TC 1 Z9 1 U1 10 U2 18 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2016 VL 114 IS 2 BP 97 EP 107 DI 10.5849/jof.14-130 PG 11 WC Forestry SC Forestry GA DG4MS UT WOS:000372046900004 ER PT J AU South, DB Harper, RA AF South, David B. Harper, Richard A. TI A Decline in Timberland Continues for Several Southern Yellow Pines SO JOURNAL OF FORESTRY LA English DT Article DE fire; forest type; natural regeneration; plantations ID APPALACHIAN MOUNTAINS; PLANTATIONS; FIRE AB During the last half of the 20th century, southern yellow pine (Pinus subsection Australes) timberland in North America declined by 16 million acres (3.6 million acres per decade). Declines in acreage in the South have continued in the 21st century for five pine forest types. In contrast, increases in timberland (due to tree planting) have occurred for both loblolly pine and longleaf pine. As a result of the practice of artificial regeneration, timberland for southern yellow pines (in the South) increased by more than 7 million acres since 1992. In contrast, relying on natural regeneration has contributed to a decline in natural pine stands for shortleaf pine, Virginia pine, slash pine, pond pine, and pitch pine. The narrative of the decline for these specific forest types is often overlooked when data for loblolly pine are pooled together with those for seven other forest types, and the total is reported as one forest type group. This article provides timberland changes that occurred in the South (1992-2012) for each of the 10 southern yellow pine forest types. C1 [South, David B.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Harper, Richard A.] US Forest Serv, Forest Inventory & Anal, USDA, Washington, DC USA. RP South, DB (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. EM southdb@auburn.edu; raharper17@bellsouth.net NR 43 TC 2 Z9 2 U1 2 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2016 VL 114 IS 2 BP 116 EP 124 DI 10.5849/jof.15-006 PG 9 WC Forestry SC Forestry GA DG4MS UT WOS:000372046900006 ER PT J AU Zald, HSJ Spies, TA Harmon, ME Twery, MJ AF Zald, Harold S. J. Spies, Thomas A. Harmon, Mark E. Twery, Mark J. TI Forest Carbon Calculators: A Review for Managers, Policymakers, and Educators SO JOURNAL OF FORESTRY LA English DT Review DE forest carbon calculators; carbon sequestration; management; disturbance ID FUEL REDUCTION TREATMENTS; WESTERN UNITED-STATES; CLIMATE-CHANGE; PACIFIC-NORTHWEST; EMISSIONS; WILDFIRE; IMPACTS; FIRE; DYNAMICS; STORAGE AB Forests play a critical role sequestering atmospheric carbon dioxide, partially offsetting greenhouse gas emissions, and thereby mitigating climate change. Forest management, natural disturbances, and the fate of carbon in wood products strongly influence carbon sequestration and emissions in the forest sector. Government policies, carbon offset and trading programs, and sustainable forestry certification programs make it increasingly important that carbon dynamics are incorporated into forest management decisionmaking. Many analytical tools (which we refer to as forest carbon calculators) have been developed to quantify carbon stores and dynamics in the forest sector, but it can be difficult for potential users to know which carbon calculator(s) may be best for any given application. We review 12 forest carbon calculators, providing a classification and synthesis to assist forest managers, policymakers, and educators. Additionally, we discuss key characteristics missing in existing forest carbon calculators that are needed for current. and future forest management decisionmaking. C1 [Zald, Harold S. J.] Oregon State Univ, Forest Ecosyst & Soc Dept, Corvallis, OR 97331 USA. [Spies, Thomas A.] US Forest Serv, USDA, Pacific NW Res Stn, Washington, DC 20252 USA. [Harmon, Mark E.] Oregon State Univ, Corvallis, OR 97331 USA. [Twery, Mark J.] US Forest Serv, USDA, Northern Res Stn, Washington, DC 20252 USA. RP Zald, HSJ (reprint author), Oregon State Univ, Forest Ecosyst & Soc Dept, Corvallis, OR 97331 USA.; Spies, TA (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Washington, DC 20252 USA.; Harmon, ME (reprint author), Oregon State Univ, Corvallis, OR 97331 USA.; Twery, MJ (reprint author), US Forest Serv, USDA, Northern Res Stn, Washington, DC 20252 USA. EM harold.zald@oregonstate.edu; tom.spies@oregonstate.edu; mark.harmon@oregonstate.edu; mark.twery@uvm.edu FU USDA Forest Service, Pacific Northwest Research Station; USDA Forest Service, Northern Research Station FX Funding in support of this article was provided by the USDA Forest Service, Pacific Northwest Research Station and Northern Research Station. We also thank four anonymous reviewers for their helpful reviews of this article. NR 44 TC 0 Z9 0 U1 5 U2 22 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAR PY 2016 VL 114 IS 2 BP 134 EP 143 DI 10.5849/jof.15-019 PG 10 WC Forestry SC Forestry GA DG4MS UT WOS:000372046900008 ER PT J AU Carroll, JE Smith, DM Gray, SM AF Carroll, J. E. Smith, D. M. Gray, S. M. TI Preferential acquisition and inoculation of PVYNTN over PVYO in potato by the green peach aphid Myzus persicae (Sulzer) SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID VIRUS-Y PVY; UNITED-STATES; SEED POTATOES; STRAIN; TRANSMISSION; CULTIVARS AB In the past decade, the incidence and distribution of the recombinant, tuber necrotic strain of Potato virus Y (PVYNTN) has been increasing in the US seed potato crop while the ordinary strain (PVYO) has been decreasing. The transmission efficiency of both strains was determined from two potato cultivars when acquired sequentially by the same aphid or when acquired by separate aphids and inoculated to the same plant. PVYNTN was transmitted more efficiently than PVYO and the order of acquisition or inoculation did not affect the preferential transmission of PVYNTN. When a recipient plant became infected with both strains, PVYNTN maintained higher titre than PVYO and would facilitate the acquisition of PVYNTN. Furthermore, the acquisition and transmission of PVYNTN over PVYO was enhanced in the potato cultivar that expressed a strain-specific Ny-like resistance gene that confers partial resistance to PVYO. C1 [Carroll, J. E.] Cornell Univ, New York State IPM Program, 630 W North St, Geneva, NY 14456 USA. [Carroll, J. E.] Cornell Univ, Sect Plant Pathol & Plant Microbe Biol, 630 W North St, Geneva, NY 14456 USA. [Smith, D. M.; Gray, S. M.] Cornell Univ, Sch Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Gray, S. M.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Gray, SM (reprint author), Cornell Univ, Sch Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA.; Gray, SM (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM smg3@cornell.edu FU USDA NIFA Specialty Crop Research Initiative (SCRI) [2009-51181-05894, 2014-51181-22373]; NSF/USDA EEID [2013-04567] FX The authors acknowledge and thank Alex Wilson, Sam Hodgson and Kaspar Kuhne for their technical support and assistance during the course of these experiments; Jason Ingram, USDA-ARS research technician, for assistance with managing plants in the greenhouse; Yu-hsuan Lin, Postdoctoral Research Associate, USDA-ARS, for testing tuber-grown plants for presence of virus mixtures and Erika Mudrak, research support specialist Statistical Consulting Unit, for guidance with statistical analyses. This research was partially funded by the USDA NIFA Specialty Crop Research Initiative (SCRI), award numbers 2009-51181-05894 and 2014-51181-22373 and NSF/USDA EEID, Grant 2013-04567. NR 20 TC 2 Z9 2 U1 3 U2 11 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD MAR PY 2016 VL 97 BP 797 EP 802 DI 10.1099/jgv.0.000374 PN 3 PG 6 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA DG8KP UT WOS:000372333000026 PM 26675892 ER PT J AU Dassanayake, RP Orru, CD Hughson, AG Caughey, B Graca, T Zhuang, DY Madsen-Bouterse, SA Knowles, DP Schneider, DA AF Dassanayake, Rohana P. Orru, Christina D. Hughson, Andrew G. Caughey, Byron Graca, Telmo Zhuang, Dongyue Madsen-Bouterse, Sally A. Knowles, Donald P. Schneider, David A. TI Sensitive and specific detection of classical scrapie prions in the brains of goats by real-time quaking-induced conversion SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID EXPERIMENTALLY CHALLENGED GOATS; CREUTZFELDT-JAKOB-DISEASE; NATURAL SCRAPIE; IMMUNOHISTOCHEMICAL DETECTION; SEEDED CONVERSION; CAPRINE SCRAPIE; LYMPHOID-TISSUE; SHEEP SCRAPIE; RECTAL MUCOSA; PROTEIN GENE AB Real-time quaking-induced conversion (RT-QuIC) is a rapid, specific and highly sensitive prion seeding activity detection assay that uses recombinant prion protein (rPrP(Sen)) to detect subinfectious levels of the abnormal isoforms of the prion protein (PrPSc). Although RT-QuIC has been successfully used to detect PrPSc in various tissues from humans and animals, including sheep, tissues from goats infected with classical scrapie have not yet been tested. Therefore, the aims of the present study were to (1) evaluate whether prion seeding activity could be detected in the brain tissues of goats with scrapie using RT-QuIC, (2) optimize reaction conditions to improve scrapie detection in goats, and (3) compare the performance of RT-QuIC for the detection of PrPSc with the more commonly used ELISA and Western blot assays. We further optimized RT-QuIC conditions for sensitive and specific detection of goat scrapie seeding activity in brain tissue from clinical animals. When used with 200 mM sodium chloride, both full-length sheep rPrP(Sen) substrates (PrP genotypes A(136)R(154)Q(171) and V(136)R(154)Q(171)) provided good discrimination between scrapie-infected and normal goat brain samples at 10(-3) dilution within 15 h. Our findings indicate that RT-QuIC was at least 10 000-fold more sensitive than ELISA and Western blot assays for the detection of scrapie seeding activity in goat brain samples. In addition to PRNP WT samples, positive RT-QuIC reactions were also observed with three PRNP polymorphic goat brain samples (G/S127, I/M142 and H/R143) tested. Taken together, these findings demonstrate that RT-QuIC sensitively detects prion seeding activity in classical scrapie-infected goat brain samples. C1 [Dassanayake, Rohana P.; Graca, Telmo; Madsen-Bouterse, Sally A.; Knowles, Donald P.; Schneider, David A.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Orru, Christina D.; Hughson, Andrew G.; Caughey, Byron] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. [Graca, Telmo] Washington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Zhuang, Dongyue; Knowles, Donald P.; Schneider, David A.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. RP Dassanayake, RP (reprint author), Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM rohana1@vetmed.wsu.edu FU US Department of Agriculture Agricultural Research Service [CRIS 2090-32000-030-00D]; Intramural Research Program of the National Institute of Allergy and Infectious Diseases FX This study was funded by the US Department of Agriculture Agricultural Research Service (CRIS 2090-32000-030-00D) and also in part by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases. We thank Katherine O'Rourke (Washington State University) for critical reading of the manuscript. The authors would also like to thank Linda Hamburg, Lori Fuller, Laetisha O'Rourke, Deborah Wolheter, Jan Luft, Karel Emma and Desiree Lesiak for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 37 TC 3 Z9 3 U1 0 U2 1 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD MAR PY 2016 VL 97 BP 803 EP 812 DI 10.1099/jgv.0.000367 PN 3 PG 10 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA DG8KP UT WOS:000372333000027 PM 26653410 ER PT J AU Asadzadeh, M Leon, L Yang, WH Bosch, D AF Asadzadeh, Masoud Leon, Luis Yang, Wanhong Bosch, David TI One-day offset in daily hydrologic modeling: An exploration of the issue in automatic model calibration SO JOURNAL OF HYDROLOGY LA English DT Article DE Hydrologic modeling; Daily simulation; Automatic calibration; Offset in daily precipitation-runoff; Nash-Sutcliffe efficiency; Kling-Gupta efficiency ID WATER ASSESSMENT-TOOL; SWAT MODEL; CLIMATE-CHANGE; QUALITY IMPACT; COASTAL-PLAIN; NASH VALUES; VALIDATION; UNCERTAINTY; SOIL; PERFORMANCE AB Hydrologic modeling literature illustrates that daily simulation models are incapable of accurately representing hydrograph timing due to relationships between precipitation and watershed hydrologic response that happen with a sub-daily time step in the real world. For watersheds with a time of concentration less than 24 h and a late day precipitation event, the observed hydrographic response frequently occurs one day after the precipitation peak while the model simulates a same day event. The analysis of sub-daily precipitation and runoff in this study suggests that, this one-day offset is inevitable in daily analysis of the precipitation-runoff relationship when the same 24-h time interval, e.g. the calendar day, is used to prepare daily precipitation and runoff datasets. Under these conditions, daily simulation models will fail to emulate this one-day offset issue (1dOI) and result in significant daily residuals between simulated and measured hydrographs. Results of this study show that the automatic calibration of such daily models will be misled by model performance metrics that are based on the aggregation of daily residuals to a solution that systematically underestimate the peak flow rates while trying to emulate the one-day lags. In this study, a novel algorithm called Shifting Hydrograph In order to Fix Timing (SHIFT) is developed to reduce the impact of this one-day offset issue (1dOI) on the parameter estimation of daily simulation models. Results show that with SHIFT the aforementioned automatic calibration finds a solution that accurately estimates the magnitude of daily peak flow rates and the shape of the rising and falling limbs of the daily hydrograph. Moreover, it is shown that this daily calibrated model performs quite well with an alternative daily precipitation dataset that has a minimal number of 1dOIs, concluding that SHIFT can minimize the impact of 1dOI on parameter estimation of daily simulation models. (C) 2016 Elsevier B.V. All rights reserved. C1 [Asadzadeh, Masoud] Univ Manitoba, Dept Civil Engn, EITC E1-306,15 Gillson St, Winnipeg, MB R3T 5V6, Canada. [Asadzadeh, Masoud; Leon, Luis] Environm Canada, Natl Water Res Inst, Burlington, ON L7R 4A6, Canada. [Yang, Wanhong] Univ Guelph, Dept Geog, 50 Stone Rd East, Guelph, ON N1G 2W1, Canada. [Bosch, David] USDA ARS, Southeast Watershed Res Lab, 2375 Rainwater Rd, Tifton, GA 31794 USA. RP Asadzadeh, M (reprint author), Univ Manitoba, Dept Civil Engn, EITC E1-306,15 Gillson St, Winnipeg, MB R3T 5V6, Canada. EM Masoud.Asadzadeh@umanitoba.ca; luis.leon@ec.gc.ca; wayang@uoguelph.ca; david.bosch@ars.usda.gov RI Asadzadeh, Masoud/F-4743-2014 OI Asadzadeh, Masoud/0000-0002-7290-7731 FU NSERC, Natural Sciences and Engineering Research Council of Canada [436591] FX This study was financially supported by NSERC, Natural Sciences and Engineering Research Council of Canada - Visiting Fellowship awarded to M. Asadzadeh (ID: 436591). We would like to thank Toronto and Region Conservation Authority for providing the sub-daily precipitation and streamflow data. Special thanks to Dr. Y. Liu for his valuable comments on setting up the SWAT model for the Rouge River watershed. NR 55 TC 1 Z9 1 U1 5 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD MAR PY 2016 VL 534 BP 164 EP 177 DI 10.1016/j.jhydrol.2015.12.056 PG 14 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DG3AC UT WOS:000371940900015 ER PT J AU Harmel, RD Hathaway, JM Wagner, KL Wolfe, JE Karthikeyan, R Francesconi, W McCarthy, DT AF Harmel, R. D. Hathaway, J. M. Wagner, K. L. Wolfe, J. E. Karthikeyan, R. Francesconi, W. McCarthy, D. T. TI Uncertainty in monitoring E. coli concentrations in streams and stormwater runoff SO JOURNAL OF HYDROLOGY LA English DT Article DE E. coli; Uncertainty; TMDL; Error propagation; Monitoring ID WATER-QUALITY DATA; ESCHERICHIA-COLI; INDICATOR BACTERIA; RIVER WATER; COLIFORMS; CRYPTOSPORIDIUM; TEMPERATURE; PHOSPHORUS; STORAGE; TIME AB Microbial contamination of surface waters, a substantial public health concern throughout the world, is typically identified by fecal indicator bacteria such as Escherichia coli. Thus, monitoring E. coil concentrations is critical to evaluate current conditions, determine restoration effectiveness, and inform model development and calibration. An often overlooked component of these monitoring and modeling activities is understanding the inherent random and systematic uncertainty present in measured data. In this research, a review and subsequent analysis was performed to identify, document, and analyze measurement uncertainty of E. coli data collected in stream flow and stormwater runoff as individual discrete samples or throughout a single runoff event. Data on the uncertainty contributed by sample collection, sample preservation/storage, and laboratory analysis in measured E. coli concentrations were compiled and analyzed, and differences in sampling method and data quality scenarios were compared. The analysis showed that: (1) manual integrated sampling produced the lowest random and systematic uncertainty in individual samples, but automated sampling typically produced the lowest uncertainty when sampling throughout runoff events; (2) sample collection procedures often contributed the highest amount of uncertainty, although laboratory analysis introduced substantial random uncertainty and preservation/storage introduced substantial systematic uncertainty under some scenarios; and (3) the uncertainty in measured E. coli concentrations was greater than that of sediment and nutrients, but the difference was not as great as may be assumed. This comprehensive analysis of uncertainty in E. coli concentrations measured in streamflow and runoff should provide valuable insight for designing E. coli monitoring projects, reducing uncertainty in quality assurance efforts, regulatory and policy decision making, and fate and transport modeling. Published by Elsevier B.V. C1 [Harmel, R. D.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Hathaway, J. M.] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA. [Wagner, K. L.] Texas A&M AgriLife Res, Texas Water Resources Inst, College Stn, TX USA. [Wolfe, J. E.] Texas A&M AgriLife Res, Blackland Res & Extens Ctr, Temple, TX USA. [Karthikeyan, R.] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX USA. [Francesconi, W.] CIAT, Lima, Peru. [McCarthy, D. T.] Monash Univ, Dept Civil Engn, EPHM Lab, Clayton Campus, Melbourne, Vic 3004, Australia. RP Harmel, RD (reprint author), USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA.; Harmel, RD (reprint author), USDA ARS, 808 E Blackland Rd, Temple, TX 76502 USA. EM daren.harmel@ars.usda.gov NR 61 TC 5 Z9 5 U1 17 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD MAR PY 2016 VL 534 BP 524 EP 533 DI 10.1016/j.jhydrol.2016.01.040 PG 10 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DG3AC UT WOS:000371940900042 ER PT J AU Davis, TJ Kline, DL Kaufman, PE AF Davis, Timothy J. Kline, Daniel L. Kaufman, Phillip E. TI Aedes albopictus (Diptera: Culicidae) Oviposition Preference as Influenced by Container Size and Buddleja davidii Plants SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Asian tiger mosquito; oviposition; egg allocation; butterfly bush; nectar feeding ID LETHAL OVITRAP; AEGYPTI DIPTERA; HEIGHT PREFERENCES; FIELD-EVALUATION; DENGUE VECTORS; MOSQUITO; FLORIDA; SUGAR; PRODUCTIVITY; POPULATIONS AB Aedes albopictus (Skuse) is a container-breeding mosquito commonly found in residential areas of its range in the United States. Mosquitoes are known to utilize flowering plants for sugar acquisition. Limited information is known about the influences on oviposition site selection, outside of container size. Residential areas are often landscaped with a variety of flowering plants and are known to provide numerous sizes of potential larval developmental sites for container-breeding mosqutioes. Through screened enclosure and field studies, the oviposition preference of Ae. albopictus for containers of three selected sizes (473, 946 and 1,892 ml) and the influence of flowering butterfly bush (Buddleja davidii Franchett cultivar 'Guinevere') plants were examined. Our results document that significantly more eggs were oviposited in the largest containers. Additionally, significantly more eggs were oviposited in containers adjacent to flowering butterfly bushes than in those without a flowering butterfly bush. Finally, our results document that flowering butterfly bushes exerted greater influence over Ae. albopictus oviposition decisions than did container size. Our findings can be applied to several aspects of Ae. albopictus surveillance and control. C1 [Davis, Timothy J.; Kaufman, Phillip E.] Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. [Kline, Daniel L.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. RP Kaufman, PE (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. EM timothy.davis.8@us.af.mil; dan.kline@ars.usda.gov; pkaufman@ufl.edu OI Kaufman, Phillip/0000-0001-6159-8358 NR 52 TC 0 Z9 0 U1 4 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2016 VL 53 IS 2 BP 273 EP 278 DI 10.1093/jme/tjv201 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DG2PA UT WOS:000371908500005 PM 26733402 ER PT J AU Puglise, JM Estep, AS Becnel, JJ AF Puglise, Jason M. Estep, Alden S. Becnel, James J. TI Expression Profiles and RNAi Silencing of Inhibitor of Apoptosis Transcripts in Aedes, Anopheles, and Culex Mosquitoes (Diptera: Culicidae) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE RNA interference; inhibitor of apoptosis; Aedes aegypti; Anopheles quadrimaculatus; Culex quinquefasciatus ID YELLOW-FEVER MOSQUITO; GENE-EXPRESSION; INSECTICIDE RESISTANCE; MALARIA VACCINES; AEGYPTI; DROSOPHILA; VECTOR; PROTEIN-1; PROGRESS; DISEASE AB Effective mosquito control is vital to curtail the devastating health effects of many vectored diseases. RNA interference (RNAi)-mediated control of mosquitoes is an attractive alternative to conventional chemical pesticides. Previous studies have suggested that transcripts for inhibitors of apoptosis (IAPs) may be good RNAi targets. To revisit and extend previous reports, we examined the expression of Aedes aegypti (L.) IAPs (AaeIAPs) 1, 2, 5, 6, 9, and a viral IAP-associated factor (vIAF) as well as Anopheles quadrimaculatus Say and Culex quinquefasciatus Say IAP1 homologs (AquIAP1 and CquIAP1) in adult females. Expression profiles of IAPs suggested that some older female mosquitoes had significantly higher IAP mRNA levels when compared to the youngest ones. Minor differences in expression of AaeIAPs were observed in mosquitoes that imbibed a bloodmeal, but the majority of the time points (up to 48 h) were not significantly different. Although in vitro experiments with the Ae. aegypti Aag-2 cell line demonstrated that the various AaeIAPs could be effectively knocked down within one day after dsRNA treatment, only Aag-2 cells treated with dsIAP1 displayed apoptotic morphology. Gene silencing and mortality were also evaluated after topical application and microinjection of the same dsRNAs into female Ae. aegypti. In contrast to previous reports, topical administration of dsRNA against AaeIAP1 did not yield a significant reduction in gene expression or increased mortality. Knockdown of IAP1 and other IAPs by microinjection did not result in significant mortality. In toto, our findings suggest that IAPs may not be suitable RNAi targets for controlling adult mosquito populations. C1 [Puglise, Jason M.; Estep, Alden S.; Becnel, James J.] USDA ARS, Ctr Med Agr & Vet Entomol, Mosquito & Fly Res Unit, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. [Estep, Alden S.] NECE, CMAVE Detachment, Naval Air Stn Jacksonville, 937 Child St, Jacksonville, FL 32211 USA. RP Estep, AS (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Mosquito & Fly Res Unit, 1600 SW 23rd Dr, Gainesville, FL 32608 USA.; Estep, AS (reprint author), NECE, CMAVE Detachment, Naval Air Stn Jacksonville, 937 Child St, Jacksonville, FL 32211 USA. EM talosian@juno.com; alden.estep@ars.usda.gov; james.becnel@ars.usda.gov FU Deployed War-Fighter Protection (DWFP) Research Program; U.S. Department of Defense (DOD) FX We sincerely thank James Baum (Monsanto Company), Paul Shirk (USDA-ARS, CMAVE), and Liming Zhao (Florida Medical Entomology Laboratory) for their critical reviews of the manuscript. We'd also like to express our gratitude to Paul Shirk for his assistance in cell imaging as well as Neil Sanscrainte and Jessica Louton (USDA-ARS, CMAVE) for excellent technical support. Additionally, we want to acknowledge the Fungal Genomics group at Broad Institute of MIT and Harvard for kindly providing us mRNA sequence data on An. quadrimaculatus and Monsanto Company for graciously furnishing us with an ample supply of dsRNAs. Funding for this work was provided by the Deployed War-Fighter Protection (DWFP) Research Program, which is sponsored by the U.S. Department of Defense (DOD) and administered by the Armed Forces Pest Management Board (AFPMB). NR 49 TC 2 Z9 2 U1 10 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2016 VL 53 IS 2 BP 304 EP 314 DI 10.1093/jme/tjv191 PG 11 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DG2PA UT WOS:000371908500009 PM 26659858 ER PT J AU Bernier, UR Clark, GG Gurman, P Elman, NM AF Bernier, Ulrich R. Clark, Gary G. Gurman, Pablo Elman, Noel M. TI The Use of Microdispensers with Spatial Repellents for Personal Protection Against Mosquito Biting SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE vector-borne disease; drug delivery; human landing collection; spatial repellent ID MUCOSAL CELLS; DEET; TRANSFLUTHRIN; FORMULATION; SAFETY AB Mosquito-borne pathogens affect millions of people worldwide. This work describes a new method to deliver spatial repellents. Functional microdispensers (FMDs) were designed to deliver spatial repellents against mosquitoes. In vivo trials showed that FMDs protect human subjects against mosquitoes by reducing 70-90% of bites received, with a protection that lasted up to 4 weeks. FMDs can be cost-effectively implemented as wearable or field-dispensed devices for local area protection, defined as a confined geographical region. C1 [Bernier, Ulrich R.; Clark, Gary G.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. [Gurman, Pablo] Univ Texas Dallas, Dept Mat Sci & Engn, 800W Campbell Rd, Richardson, TX 75080 USA. [Elman, Noel M.] MIT, Inst Soldier Nanotechnol, 500 Technol Sq,NE47-525, Cambridge, MA 02139 USA. [Elman, Noel M.] Charles Stark Draper Labs, Div Mat, Bio Grp, 555 Technol Sq, Cambridge, MA 02139 USA. [Elman, Noel M.] Massachusetts Gen Hosp, Ctr Innovat Care Delivery, 275 Cambridge St, Boston, MA 02114 USA. RP Elman, NM (reprint author), MIT, Inst Soldier Nanotechnol, 500 Technol Sq,NE47-525, Cambridge, MA 02139 USA.; Elman, NM (reprint author), Charles Stark Draper Labs, Div Mat, Bio Grp, 555 Technol Sq, Cambridge, MA 02139 USA.; Elman, NM (reprint author), Massachusetts Gen Hosp, Ctr Innovat Care Delivery, 275 Cambridge St, Boston, MA 02114 USA. EM uli.bernier@ars.usda.gov; roofus209@yahoo.com; pablogurman@gmail.com; noel.elman@gmail.com FU Department of Defense Deployed Warfighter Protection Program [W911QY-12-1-0005] FX We acknowledge Gregory M. Allen of United States Department of Agriculture-Agriculture Research Service-Center for Medical, Agricultural and Veterinary Entomology (USDA-ARS-CMAVE) for conducting the study with the FMDs against laboratory mosquitoes. This research work was partially supported by the Department of Defense Deployed Warfighter Protection Program (contract: W911QY-12-1-0005). NR 11 TC 0 Z9 0 U1 1 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAR PY 2016 VL 53 IS 2 BP 470 EP 472 DI 10.1093/jme/tjv190 PG 3 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DG2PA UT WOS:000371908500027 PM 26637385 ER PT J AU Pentieva, K Selhub, J Paul, L Molloy, AM McNulty, B Ward, M Marshall, B Dornan, J Reilly, R Parle-McDermott, A Bradbury, I Ozaki, M Scott, JM McNulty, H AF Pentieva, Kristina Selhub, Jacob Paul, Ligi Molloy, Anne M. McNulty, Breige Ward, Mary Marshall, Barry Dornan, James Reilly, Rosie Parle-McDermott, Anne Bradbury, Ian Ozaki, Mari Scott, John M. McNulty, Helene TI Evidence from a Randomized Trial That Exposure to Supplemental Folic Acid at Recommended Levels during Pregnancy Does Not Lead to Increased Unmetabolized Folic Acid Concentrations in Maternal or Cord Blood SO JOURNAL OF NUTRITION LA English DT Article DE pregnancy; folic acid supplementation; folic acid fortified foods; plasma; unmetabolized folic acid and total folate; maternal and cord blood ID MANDATORY FORTIFICATION; MICROBIOLOGICAL ASSAY; LACTATING WOMEN; FOLATE-STATUS; SERUM; PLASMA; ADULTS; REDUCTASE; VITAMIN-B-12; HOMOCYSTEINE AB Background: Exposure to higher intakes of folic acid (FA) from fortified foods and supplements, although largely considered beneficial, is associated with unmetabolized FA in the circulation, which has raised some health concerns. Objective: The effect of supplemental FA at a dose of 400 mg/d during pregnancy on unmetabolized FA concentrations in maternal plasma and newborn cord blood plasma was investigated. Methods: A new analysis was performed of blood samples from participants in a randomized trial in pregnancy. Women aged 18-35 y, who had taken 400 mu g FA/d as recommended in the first trimester, were recruited at the start of trimester 2 and randomly allocated to receive either 400 mg FA/d (n = 59) or a placebo (n = 67) throughout the second and third trimesters until delivery. Unmetabolized FA concentrations in maternal and cord blood samples were measured by LC-tandem MS analysis. Results: In response to the intervention from gestationalweek 14 through delivery, a higher proportion of women in the FA compared with the placebo group had detectable FA(>= 0.27 nmol/L) in plasma, but the difference in concentrations was not statistically significant (mean +/- SD: 0.44 +/- 0.80 compared with 0.13 +/- 0.49 nmol/L, P = 0.38). FA treatment throughout pregnancy resulted in higher cord blood plasma total folate (50.6 +/- 20.1 compared with 34.5 +/- 14.4 nmol/L; P = 0.004) and 5-methyltetrahydrofolate (50.4 +/- 20.3 compared with 34.5 +/- 14.4 nmol/L; P = 0.005) concentrations, but FA was detected only in 8 of 53 available cord blood samples, and the proportion of samples with detectable FA concentrations was similar in FA-treated and placebo groups. Conclusions: Plasma concentrations of unmetabolized FA arising from supplemental FA at a dose of 400 mg/d, in addition to FA from fortified foods, were low or undetectable in mothers and newborns. The benefits for mothers and offspring of continuing FA supplementation beyond the first trimester of pregnancy can be achieved without posing any risk of increasing unmetabolized circulating FA, even in those already exposed to FA from fortified foods. C1 [Pentieva, Kristina; Ward, Mary; Reilly, Rosie; McNulty, Helene] Univ Ulster, Northern Ireland Ctr Food & Hlth NICHE, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland. [Selhub, Jacob; Paul, Ligi] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Vitamin Metab & Aging Lab, Boston, MA 02111 USA. [Molloy, Anne M.] Trinity Coll Dublin, Sch Med, Dublin, Ireland. [Scott, John M.] Trinity Coll Dublin, Sch Biochem & Immunol, Dublin, Ireland. [McNulty, Breige] Univ Coll Dublin, Sch Agr & Food Sci, Dublin 2, Ireland. [Marshall, Barry] Causeway Hosp, Northern Hlth & Social Care Trust, Coleraine, Londonderry, North Ireland. [Dornan, James] Royal Jubilee Matern Serv, Belfast, Antrim, North Ireland. [Parle-McDermott, Anne; Ozaki, Mari] Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland. [Bradbury, Ian] Frontier Sci Scotland, Kincraig, Scotland. RP Pentieva, K (reprint author), Univ Ulster, Northern Ireland Ctr Food & Hlth NICHE, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland. EM k.pentieva@ulster.ac.uk OI Ward, Mary/0000-0001-6470-1728; Pentieva, Kristina/0000-0003-2059-534X FU Northern Ireland Department for Employment and Learning; Health Research Board of Ireland [HRA_PHS/2010/20]; HSC Research and Development Division of the Public Health Agency, Northern Ireland [STL/5043/14] FX Supported by the Northern Ireland Department for Employment and Learning (PhD funding for B McNulty), the Health Research Board of Ireland (HRA_PHS/2010/20), and the HSC Research and Development Division of the Public Health Agency, Northern Ireland (Enabling Research Award STL/5043/14). NR 33 TC 5 Z9 5 U1 1 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAR 1 PY 2016 VL 146 IS 3 BP 494 EP 500 DI 10.3945/jn.115.223644 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DG6ME UT WOS:000372198200004 PM 26817717 ER PT J AU Shypailo, RJ AF Shypailo, Roman J. TI Stability evaluation and correction of a pulsed neutron generator prompt gamma activation analysis system SO JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY LA English DT Article; Proceedings Paper CT 10th International Conference on Methods and Applications of Radioanalytical Chemistry (MARC) CY APR 12-17, 2015 CL Kailua Kona, HI DE Body composition; Neutron activation analysis; Nitrogen; Humans; Neutron generator ID TOTAL-BODY NITROGEN AB Source output stability is important for accurate measurement in prompt gamma neutron activation. This is especially true when measuring low-concentration elements such as in vivo nitrogen (similar to 2.5 % of body weight). We evaluated the stability of the compact DT neutron generator within an in vivo nitrogen measurement system. Review of gamma event/time patterns and data from an auxiliary detector showed significant variations among repeated phantom runs. Neutron generator instability had a significant effect on measurement precision. The neutron generator used in our system must be monitored for output consistency. Adjustments must be made to measurement results to correct for generator instability. C1 [Shypailo, Roman J.] USDA ARS, Dept Pediat, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. RP Shypailo, RJ (reprint author), USDA ARS, Dept Pediat, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM shypailo@bcm.edu NR 15 TC 0 Z9 0 U1 3 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0236-5731 EI 1588-2780 J9 J RADIOANAL NUCL CH JI J. Radioanal. Nucl. Chem. PD MAR PY 2016 VL 307 IS 3 BP 1781 EP 1786 DI 10.1007/s10967-015-4440-7 PG 6 WC Chemistry, Analytical; Chemistry, Inorganic & Nuclear; Nuclear Science & Technology SC Chemistry; Nuclear Science & Technology GA DG1ER UT WOS:000371808900038 ER PT J AU Angadi, SV Gowda, PH Cutforth, HW Idowu, OJ AF Angadi, Sangamesh V. Gowda, Prasanna H. Cutforth, Herb W. Idowu, O. John TI Circles of live buffer strips in a center pivot to improve multiple ecosystem services and sustainability of irrigated agriculture in the southern Great Plains SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article ID WINDBREAKS; FIELD; EVAPORATION; SYSTEMS; YIELDS; BARE C1 [Angadi, Sangamesh V.] New Mexico State Univ, Dept Plant & Environm Sci, Clovis, NM USA. [Angadi, Sangamesh V.] New Mexico State Univ, Agr Sci Ctr Clovis, Clovis, NM USA. [Gowda, Prasanna H.] ARS, USDA, Grazinglands Res Lab, El Reno, OK USA. [Cutforth, Herb W.] Agr & Agri Food Canada, Swift Current Res & Dev Ctr, Swift Current, SK, Canada. [Idowu, O. John] New Mexico State Univ, Dept Extens Plant Sci, Las Cruces, NM 88003 USA. RP Angadi, SV (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Clovis, NM USA.; Angadi, SV (reprint author), New Mexico State Univ, Agr Sci Ctr Clovis, Clovis, NM USA. NR 23 TC 0 Z9 0 U1 3 U2 6 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2016 VL 71 IS 2 BP 44A EP 49A DI 10.2489/jswc.71.2.44A PG 6 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DG2ST UT WOS:000371918800003 ER PT J AU Wren, DG Rigby, JR Davidson, GR Locke, MA AF Wren, D. G. Rigby, J. R. Davidson, G. R. Locke, M. A. TI Determination of lake sediment accumulation rates in an agricultural watershed using lead-210 and cesium-137 SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE erosion; radioisotope analysis; sediment accumulation; soil conservation ID CONSERVATION PRACTICES; EROSION CONTROL; QUALITY; RESUSPENSION; CATCHMENT; RESERVOIR; QUANTIFY; LAND; SOIL AB Quantifying the effectiveness of erosion control practices in watersheds remains a difficult problem. Determination of recent sediment accumulation rates for lake sediments in agricultural watersheds using radioisotopes, such as lead-210 (Pb-210) and cesium-137 (Cs-137), is potentially a valuable means of assessing the effectiveness of soil conservation practices at the watershed scale. The predominance of sediment arriving in runoff from the watershed, variable sedimentation rates, and mechanical mixing of soil in nearby fields all present challenges in the conversion of radioisotope data to sedimentation rates. Four sediment cores from Beasley Lake, Mississippi, were used to demonstrate the application of the Constant Initial Concentration (CIC) model for calculating sediment age from the distribution of Pb-210 bottom sediments. The activity of Cs-137 was used to supplement the Pb-210 data by providing a benchmark date within the core to calibrate the CIC model. Three of the four cores showed reductions in sediment accumulation rate within the 30 years prior to core collection in 2008 and 2011 by at least 50% relative to rates from before the adoption of soil conservation measures in the watershed. The most recently resolved rates were approximately 0.5 cm y(-1) (0.2 in yr(-1)). The study demonstrates the application of the CIC model for developing sediment accumulation chronologies in agricultural catchments. C1 [Wren, D. G.; Rigby, J. R.; Locke, M. A.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS USA. [Davidson, G. R.] Univ Mississippi, Dept Geol & Geol Engn, Oxford, MS USA. RP Wren, DG (reprint author), ARS, USDA, Natl Sedimentat Lab, Oxford, MS USA. NR 35 TC 1 Z9 1 U1 5 U2 17 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2016 VL 71 IS 2 BP 137 EP 147 DI 10.2489/jswc.71.2.137 PG 11 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DG2ST UT WOS:000371918800009 ER PT J AU Teague, WR Apfelbaum, S Lal, R Kreuter, UP Rowntree, J Davies, CA Conser, R Rasmussen, M Hatfield, J Wang, T Wang, F Byck, P AF Teague, W. R. Apfelbaum, S. Lal, R. Kreuter, U. P. Rowntree, J. Davies, C. A. Conser, R. Rasmussen, M. Hatfield, J. Wang, T. Wang, F. Byck, P. TI The role of ruminants in reducing agriculture's carbon footprint in North America SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE carbon sequestration; conservation agriculture; ecosystem services; greenhouse gases; regenerative ecosystem management; soil erosion ID SOIL CARBON; CLIMATE-CHANGE; PRODUCTION SYSTEMS; FOOD SECURITY; SEQUESTRATION; EMISSIONS; FERTILIZERS; EFFICIENCY; LIVESTOCK; NITROGEN AB Owing to the methane (CH4) produced by rumen fermentation, ruminants are a source of greenhouse gas (GHG) and are perceived as a problem. We propose that with appropriate regenerative crop and grazing management, ruminants not only reduce overall GHG emissions, but also facilitate provision of essential ecosystem services, increase soil carbon (C) sequestration, and reduce environmental damage. We tested our hypothesis by examining biophysical impacts and the magnitude of all GHG emissions from key agricultural production activities, including comparisons of arable-and pastoral-based agroecosystems. Our assessment shows that globally, GHG emissions from domestic ruminants represent 11.6% (1.58 Gt C y(-1)) of total anthropogenic emissions, while cropping and soil-associated emissions contribute 13.7% (1.86 Gt C y(-1)). The primary source is soil erosion (1 Gt C y(-1)), which in the United States alone is estimated at 1.72 Gt of soil y(-1). Permanent cover of forage plants is highly effective in reducing soil erosion, and ruminants consuming only grazed forages under appropriate management result in more C sequestration than emissions. Incorporating forages and ruminants into regeneratively managed agroecosystems can elevate soil organic C, improve soil ecological function by minimizing the damage of tillage and inorganic fertilizers and biocides, and enhance biodiversity and wildlife habitat. We conclude that to ensure longterm sustainability and ecological resilience of agroecosystems, agricultural production should be guided by policies and regenerative management protocols that include ruminant grazing. Collectively, conservation agriculture supports ecologically healthy, resilient agroecosystems and simultaneously mitigates large quantities of anthropogenic GHG emissions. C1 [Teague, W. R.; Wang, T.] Texas A&M AgriLife Res, Vernon, TX USA. [Teague, W. R.; Kreuter, U. P.] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX USA. [Apfelbaum, S.; Wang, F.] Appl Ecol Serv Inc, Brodhead, WI USA. [Lal, R.] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Rowntree, J.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Davies, C. A.] Shell Int Explorat & Prod Inc, Shell Technol Ctr Houston, Houston, TX USA. [Conser, R.] Regenov8 Advisors, Fulshear, TX USA. [Rasmussen, M.] Iowa State Univ, Leopold Ctr Sustainable Agr, Ames, IA USA. [Hatfield, J.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA USA. [Byck, P.] Arizona State Univ, Wrigley GlobalInst Sustainabil & Cronkit Sch Jour, Tempe, AZ USA. [Byck, P.] Arizona State Univ, Wrigley GlobalInst Sustainabil & Cronkit Sch Jour, Phoenix, AZ USA. RP Teague, WR (reprint author), Texas A&M AgriLife Res, Vernon, TX USA. NR 42 TC 0 Z9 0 U1 22 U2 45 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAR-APR PY 2016 VL 71 IS 2 BP 156 EP 164 DI 10.2489/jswc.71.2.156 PG 9 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DG2ST UT WOS:000371918800011 ER PT J AU Zwart, JA Craig, N Kelly, PT Sebestyen, SD Solomon, CT Weidel, BC Jones, SE AF Zwart, Jacob A. Craig, Nicola Kelly, Patrick T. Sebestyen, Stephen D. Solomon, Christopher T. Weidel, Brian C. Jones, Stuart E. TI Metabolic and physiochemical responses to a whole-lake experimental increase in dissolved organic carbon in a north-temperate lake SO LIMNOLOGY AND OCEANOGRAPHY LA English DT Article ID ECOSYSTEM METABOLISM; PRIMARY PRODUCTIVITY; WATER COLUMN; TERRESTRIAL; PHOSPHORUS; MATTER; DEPOSITION; EXPORT; MINERALIZATION; ZOOPLANKTON AB Over the last several decades, many lakes globally have increased in dissolved organic carbon (DOC), calling into question how lake functions may respond to increasing DOC. Unfortunately, our basis for making predictions is limited to spatial surveys, modeling, and laboratory experiments, which may not accurately capture important whole-ecosystem processes. In this article, we present data on metabolic and physio-chemical responses of a multiyear experimental whole-lake increase in DOC concentration. Unexpectedly, we observed an increase in pelagic gross primary production, likely due to a small increase in phosphorus as well as a surprising lack of change in epilimnetic light climate. We also speculate on the importance of lake size modifying the relationship between light climate and elevated DOC. A larger increase in ecosystem respiration resulted in an increased heterotrophy for the treatment basin. The magnitude of the increase in heterotrophy was extremely close to the excess DOC load to the treatment basin, indicating that changes in heterotrophy may be predictable if allochthonous carbon loads are well-constrained. Elevated DOC concentration also reduced thermocline and mixed layer depth and reduced whole-lake temperature. Results from this experiment were quantitatively different, and sometimes even in the opposite direction, from expectations based on cross-system surveys and bottle experiments, emphasizing the importance of whole-ecosystem experiments in understanding ecosystem response to environmental change. C1 [Zwart, Jacob A.; Kelly, Patrick T.; Jones, Stuart E.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Craig, Nicola; Solomon, Christopher T.] McGill Univ, Dept Nat Resource Sci, Montreal, PQ, Canada. [Sebestyen, Stephen D.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN USA. [Weidel, Brian C.] US Geol Survey, Great Lakes Sci Ctr, Lake Ontario Biol Stn, Oswego, NY USA. RP Zwart, JA (reprint author), Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. EM jzwart@nd.edu RI Sebestyen, Stephen/D-1238-2013; Solomon, Chris/E-6284-2014 OI Sebestyen, Stephen/0000-0002-6315-0108; Solomon, Chris/0000-0002-2850-4257 FU National Science Foundation [NSF DGE-1313583]; Natural Sciences and Engineering Research Council of Canada [402530-2011]; Canada Foundation for Innovation [28196] FX We thank the University of Notre Dame Environmental Research Center (UNDERC) for hosting our experiment. The chemical analyses were conducted at the Center for Environmental Science and Technology (CEST) at University of Notre Dame. Technical assistance was provided by J.J. Coloso, K. Baglini, R. Pilla, A. Sumner, S. Godwin, K. Creamer, A. Searle, K. Roberts, L. Raaf, E. Golebie, B. Conner, S. McCarthy, E. Mather, S. Elser, C.J. Humes, J. Lerner, and M.F. Ebenezer. We are extremely grateful to C. Buelo, S.R. Carpenter, J.J. Cole, J.T. Kurtzweil, and M.L. Pace for contributing Paul Lake high-frequency data for metabolism estimates in exchange for the small price of bourbon. J. R. Corman, A. L. Hetherington, and two anonymous reviewers' comments significantly improved the manuscript. This work was supported by the National Science Foundation Graduate Research Fellowship under NSF DGE-1313583 to JAZ and grants from the Natural Sciences and Engineering Research Council of Canada 402530-2011 and the Canada Foundation for Innovation 28196 to CTS. Mention of specific product or trade names does not constitute endorsement by the U.S. Government. This is contribution number 1999 to the USGS Great Lakes Science Center. NR 65 TC 2 Z9 2 U1 7 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-3590 EI 1939-5590 J9 LIMNOL OCEANOGR JI Limnol. Oceanogr. PD MAR PY 2016 VL 61 IS 2 BP 723 EP 734 DI 10.1002/lno.10248 PG 12 WC Limnology; Oceanography SC Marine & Freshwater Biology; Oceanography GA DG6BF UT WOS:000372166500017 ER PT J AU Chen, CY Nguyen, LHT Cottrell, BJ Irwin, PL Uhlich, GA AF Chen, Chin-Yi Nguyen, Ly-Huong T. Cottrell, Bryan J. Irwin, Peter L. Uhlich, Gaylen A. TI Multiple mechanisms responsible for strong Congo-red-binding variants of Escherichia coli O157:H7 strains SO PATHOGENS AND DISEASE LA English DT Article DE STEC; O157:H7; Congo red; biofilm; rcsB; mlrA ID BIOFILM FORMATION; CSGD PROMOTER; SALMONELLA-TYPHIMURIUM; EXTRACELLULAR-MATRIX; CURLI EXPRESSION; SHIGA; O157-H7; O157/H7; MLRA; RESISTANCE AB High variability in the expression of csgD-dependent, biofilm-forming and adhesive properties is common among Shiga toxin-producing Escherichia coli. Although many strains of serotype O157:H7 form little biofilm, conversion to stronger biofilm phenotypes has been observed. In this study, we screened different strains of serotype O157:H7 for the emergence of strong Congo-red (CR) affinity/biofilm-forming properties and investigated the underlying genetic mechanisms. Two major mechanisms which conferred stronger biofilm phenotypes were identified:mutations (insertion, deletion, single nucleotide change) in rcsB region and stx-prophage excision from the mlrA site. Restoration of the native mlrA gene (due to prophage excision) resulted in strong biofilm properties to all variants. Whereas RcsB mutants showed weaker CR affinity and biofilm properties, it provided more possibilities for phenotypic presentations through heterogenic sequence mutations. C1 [Chen, Chin-Yi; Nguyen, Ly-Huong T.; Cottrell, Bryan J.; Irwin, Peter L.; Uhlich, Gaylen A.] ARS, USDA, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, Wyndmoor, PA 19038 USA. RP Chen, CY (reprint author), ARS, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM chin-yi.chen@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service, National Program 108 Food Safety [CRIS] [8072-42000-067] FX This work was supported by United States Department of Agriculture, Agricultural Research Service, National Program 108 Food Safety [CRIS # 8072-42000-067]. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 41 TC 3 Z9 3 U1 2 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 2049-632X J9 PATHOG DIS JI Pathog. Dis. PD MAR PY 2016 VL 74 IS 2 AR ftv123 DI 10.1093/femspd/ftv123 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DG2VY UT WOS:000371929200010 ER PT J AU Cowger, C Parks, R Kosman, E AF Cowger, Christina Parks, Ryan Kosman, Evsey TI Structure and Migration in US Blumeria graminis f. sp tritici Populations SO PHYTOPATHOLOGY LA English DT Article ID WHEAT POWDERY MILDEW; WINTER-WHEAT; LINKAGE DISEQUILIBRIUM; SPATIAL AGGREGATION; MICROSATELLITE LOCI; CONTINENTAL-SCALE; AERIAL DISPERSAL; NORTH-CAROLINA; PLANT-DISEASE; VIRULENCE AB While wheat powdery mildew occurs throughout the south-central and eastern United States, epidemics are especially damaging in the Mid-Atlantic states. The structure of the U.S. Blumeria graminis f. sp. tritici population was assessed based on a sample of 238 single-spored isolates. The isolates were collected from 16 locations in 12 states (18 site-years) as chasmothecial samples in 2003 or 2005, or as conidial samples in 2007 or 2010. DNA was evaluated using nine single nucleotide polymorphism (SNP) markers in four housekeeping genes, and 10 simple sequence repeat (SSR) markers. The SSR markers were variably polymorphic, with allele numbers ranging from 3 to 39 per locus. Genotypic diversity was high (210 haplotypes) and in eight of the site-years, every isolate had a different SSR genotype. SNP haplotypic diversity was lower; although 15 haplotypes were identified, the majority of isolates possessed one of two haplotypes. The chasmothecial samples showed no evidence of linkage disequilibrium (P = 0.36), while the conidial samples did (P = 0.001), but the two groups had nearly identical mean levels of genetic diversity, which was moderate. There was a weakly positive relationship between genetic distance and geographic distance (R-2 = 0.25, P = 0.001), indicating modest isolation by distance. Most locations in the Mid-Atlantic and Great Lakes regions clustered together genetically, while Southeast locations formed a distinct but adjacent cluster; all of these were genetically separated from Southern Plains locations and an intermediate location in Kentucky. One-way migration was detected at a rate of approximately five individuals per generation from populations west of the Appalachian Mountains to those to the east, despite the fact that the Atlantic states experience more frequent and damaging wheat mildew epidemics. Overall, the evidence argues for a large-scale mosaic of overlapping populations that re-establish themselves from local sources, rather than continental scale extinction and re-establishment, and a low rate of long-distance dispersal roughly from west to east, consistent with prevailing wind directions. C1 [Cowger, Christina; Parks, Ryan] N Carolina State Univ, USDA ARS, Dept Plant Pathol, CB7616, Raleigh, NC 27695 USA. [Kosman, Evsey] Tel Aviv Univ, Fac Life Sci, Inst Cereal Crops Improvement, IL-69978 Tel Aviv, Israel. RP Cowger, C (reprint author), N Carolina State Univ, USDA ARS, Dept Plant Pathol, CB7616, Raleigh, NC 27695 USA. EM Christina.Cowger@ars.usda.gov NR 60 TC 1 Z9 1 U1 5 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAR PY 2016 VL 106 IS 3 BP 295 EP 304 DI 10.1094/PHYTO-03-15-0066-R PG 10 WC Plant Sciences SC Plant Sciences GA DG6QR UT WOS:000372211700009 PM 26623997 ER PT J AU Mico, V Martin, R Lasuncion, MA Ordovas, JM Daimiel, L AF Mico, Victor Martin, Roberto Lasuncion, Miguel A. Ordovas, Jose M. Daimiel, Lidia TI Unsuccessful Detection of Plant MicroRNAs in Beer, Extra Virgin Olive Oil and Human Plasma After an Acute Ingestion of Extra Virgin Olive Oil SO PLANT FOODS FOR HUMAN NUTRITION LA English DT Article DE Beer; Extra virgin olive oil; Plant microRNAs; Next generation sequencing ID CROSS-KINGDOM REGULATION; MEDITERRANEAN DIET; MILK; EXPRESSION; PROVIDE; MIRNAS AB The recent description of the presence of exogenous plant microRNAs from rice in human plasma had profound implications for the interpretation of microRNAs function in human health. If validated, these results suggest that food should not be considered only as a macronutrient and micronutrient supplier but it could also be a way of genomic interchange between kingdoms. Subsequently, several studies have tried to replicate these results in rice and other plant foods and most of them have failed to find plant microRNAs in human plasma. In this scenario, we aimed to detect plant microRNAs in beer and extra virgin olive oil (EVOO) - two plant-derived liquid products frequently consumed in Spain - as well as in human plasma after an acute ingestion of EVOO. Our hypothesis was that microRNAs present in beer and EVOO raw material could survive manufacturing processes, be part of these liquid products, be absorbed by human gut and circulate in human plasma. To test this hypothesis, we first optimized the microRNA extraction protocol to extract microRNAs from beer and EVOO, and then tried to detect microRNAs in those samples and in plasma samples of healthy volunteers after an acute ingestion of EVOO. C1 [Mico, Victor; Martin, Roberto; Ordovas, Jose M.; Daimiel, Lidia] CEI UAM CSIC, Fdn IMDEA Alimentac, Genom Nutr Enfermedad Cardiovasc & Obesidad, Madrid 28049, Spain. [Lasuncion, Miguel A.] Hosp Univ Ramon y Cajal, Serv Bioquim Invest, Inst Ramon y Cajal Invest Sanitaria, E-28034 Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Daimiel, L (reprint author), CEI UAM CSIC, Fdn IMDEA Alimentac, Genom Nutr Enfermedad Cardiovasc & Obesidad, Madrid 28049, Spain. EM lidia.daimiel@imdea.org RI Lasuncion, Miguel A./K-6167-2014; Martin-Hernandez, Roberto/M-8161-2014; OI Lasuncion, Miguel A./0000-0003-0299-9391; Daimiel-Ruiz, Lidia Angeles/0000-0001-9898-6629; Martin-Hernandez, Roberto/0000-0002-2723-6289; Mico, Victor/0000-0003-0681-7962 FU Fundacion Cerveza y Salud, Fundacion Salud - Merck-Serono research fellowship; Instituto de Salud Carlos III [PI14/01374]; Manuel de Oya fellowship of the Fundacion Cerveza y Salud FX The present work was funded by the Fundacion Cerveza y Salud, Fundacion Salud 2000 - Merck-Serono research fellowship and Instituto de Salud Carlos III (PI14/01374). VM was supported by a Manuel de Oya fellowship of the Fundacion Cerveza y Salud. LD and JMO conceived the study idea and designed the research. VM carried out experimental approaches. LD and MAL conducted the intervention study. RM and LD carried out the sequencing analyses. LD and VM wrote the manuscript and all authors assisted in manuscript revision and approved it. NR 21 TC 2 Z9 2 U1 7 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-9668 EI 1573-9104 J9 PLANT FOOD HUM NUTR JI Plant Food Hum. Nutr. PD MAR PY 2016 VL 71 IS 1 BP 102 EP 108 DI 10.1007/s11130-016-0534-9 PG 7 WC Plant Sciences; Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Plant Sciences; Chemistry; Food Science & Technology; Nutrition & Dietetics GA DG7UZ UT WOS:000372290500015 PM 26872816 ER PT J AU Wilson, KM Bourassa, DV Davis, AJ Freeman, ME Buhr, RJ AF Wilson, K. M. Bourassa, D. V. Davis, A. J. Freeman, M. E. Buhr, R. J. TI The addition of charcoals to broiler diets did not alter the recovery of Salmonella Typhimurium during grow-out SO POULTRY SCIENCE LA English DT Article DE Salmonella; broilers; charcoal; ceca; feed ID CHAIN FATTY-ACIDS; CAMPYLOBACTER-JEJUNI COLONIZATION; INCREMENTAL FEED WITHDRAWAL; ACTIVATED-CHARCOAL; BACTERIA RECOVERY; INTESTINAL COLONIZATION; MICROBIOLOGICAL CHANGES; GASTROINTESTINAL PH; ESCHERICHIA-COLI; DRINKING-WATER AB Two experiments evaluated prebiotics added to feed on the recovery of Salmonella in broilers during grow-out and processing. In Experiment 1, "seeder" chicks were inoculated with Salmonella Typhimurium and placed with penmates. Treatments were: basal control diet, added 0.3% bamboo charcoal, 0.6% bamboo charcoal, or 0.12% Aromabiotic (medium chain fatty acids). The ceca from seeders and penmates were sampled to confirm Salmonella colonization at 3, 4, and 6 wk, and pen litter was sampled weekly. At 3 wk, charcoal fed chicks had significantly lower cecal recovery (37% lower) of Salmonella via direct plating but no differences at wk 4 or 6. At 6 wk, broilers fed Aromabiotic had no recovery of Salmonella from ceca with direct plating and significantly, 18%, lower recovery with enrichment. In Experiment 2, the treatments were: basal control diet, added 0.3% bamboo charcoal, 0.3% activated bamboo charcoal, or 0.3% pine charcoal. At placement, 2 seeders were challenged with Salmonella and commingled with penmates and ceca sampled at 1 and 2 wk, and ceca from 5 penmates/pen at 3 to 6 wk. Weekly, the pH of the crop and duode-num was measured from 1 penmate/pen and the litter surface sampled. At the end of grow-out broilers were processed. Results showed that penmates had colonized at 1 and 2 wk. Cecal Salmonella showed no differences except at 4 wk, when activated bamboo charcoal had a 18% lower recovery of Salmonella (enrichment) compared to the control (88%). Similar to Experiment 1, the recovery of Salmonella from the litter was not significantly different among treatments, however an overall decrease in recovery by 4 wk with direct plating reoccurred. The pH of the duodenum and the crop were not different among treatments. Crop pH (6.0) for all treatments were significantly higher at wk 1 compared to wk 2 to 6. Charcoals had minimal effect on Salmonella recovery in the ceca, but following defeathering, broilers fed charcoals had significantly lower Salmonella recovery from breast skin (charcoals 5+/60 compared to control 8+/20). While the addition of charcoals to broilers feed did not significantly affect Salmonella recovery during production (from litter or ceca samples) there was a lower Salmonella recovery from breast skin following scalding and defeathering. C1 [Wilson, K. M.; Davis, A. J.; Freeman, M. E.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. [Wilson, K. M.; Bourassa, D. V.; Buhr, R. J.] USDA ARS, Poultry Microbiol Safety & Proc Res Unit, US Natl Poultry Res Ctr, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. RP Buhr, RJ (reprint author), USDA ARS, Poultry Microbiol Safety & Proc Res Unit, US Natl Poultry Res Ctr, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. EM jeff.buhr@ars.usda.gov FU USDA-ARS CRIS Project [6040-32000-068-00]; University of Georgia; EcoPlanet Bamboo FX The authors are grateful for the assistance provided by Jeromey S. Jackson of the USDA-ARS Richard B. Russell Agricultural Research Center, Ashley Stephens, Caitlin Harris, Mathew Jones and Josh Steed of The University of Georgia for assistance with sample collection and plating. Financial support provided by USDA-ARS CRIS Project Number 6040-32000-068-00, the University of Georgia, and EcoPlanet Bamboo. NR 67 TC 1 Z9 1 U1 2 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAR PY 2016 VL 95 IS 3 BP 694 EP 704 DI 10.3382/ps/pev371 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DG4XC UT WOS:000372075400025 PM 26755657 ER PT J AU Gillan, JK Karl, JW Barger, NN Elaksher, A Duniway, MC AF Gillan, Jeffrey K. Karl, Jason W. Barger, Nichole N. Elaksher, Ahmed Duniway, Michael C. TI Spatially Explicit Rangeland Erosion Monitoring Using High-Resolution Digital Aerial Imagery SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE change detection; digital elevation model; photogrammetry; rangeland monitoring; remote sensing; soil erosion ID MEASURING SOIL-EROSION; ELEVATION MODELS; GULLY-EROSION; LIDAR DATA; PHOTOGRAMMETRIC TECHNIQUE; FOREST CANOPY; NE-SPAIN; DEPOSITION; RATES; QUANTIFICATION AB Nearly all of the ecosystem services supported by rangelands, including production of livestock forage, carbon sequestration, and provisioning of clean water, are negatively impacted by soil erosion. Accordingly, monitoring the severity, spatial extent, and rate of soil erosion is essential for long-term sustainable management. Traditional field-based methods of monitoring erosion (sediment traps, erosion pins, and bridges) can be labor intensive and therefore are generally limited in spatial intensity and/or extent. There is a growing effort to monitor natural resources at broad scales, which is driving the need for new soil erosion monitoring tools. One remote-sensing technique that can be used to monitor soil movement is a time series of digital elevation models (DEMs) created using aerial photogrammetry methods. By geographically coregistering the DEMs and subtracting one surface from the other, an estimate of soil elevation change can be created. Such analysis enables spatially explicit quantification and visualization of net soil movement including erosion, deposition, and redistribution. We constructed DEMs (12-cm ground sampling distance) on the basis of aerial photography immediately before and 1 year after a vegetation removal treatment on a 31-ha Pinon-Juniper woodland in southeastern Utah to evaluate the use of aerial photography in detecting soil surface change. On average, we were able to detect surface elevation change of +/- 8-9cm and greater, which was sufficient for the large amount of soil movement exhibited on the study area. Detecting more subtle soil erosion could be achieved using the same technique with higher resolution imagery from lower-flying aircraft such as unmanned aerial vehicles. DEM differencing and process focused field methods provided complementary information and a more complete assessment of soil loss and movement than any single technique alone. Photogrammetric DEM differencing could be used as a technique to quantitatively monitor surface change over time relative to management activities. (C) 2016 The Society for Range Management. Published by Elsevier Inc. All rights reserved. C1 [Gillan, Jeffrey K.; Karl, Jason W.] New Mexico State Univ, USDA ARS, Jornada Expt Range, Las Cruces, NM 88003 USA. [Barger, Nichole N.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Elaksher, Ahmed] New Mexico State Univ, Dept Engn Technol & Surveying Engn, Las Cruces, NM 88003 USA. [Duniway, Michael C.] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT 84532 USA. RP Gillan, JK (reprint author), New Mexico State Univ, USDA ARS, Jornada Expt Range, Las Cruces, NM 88003 USA. EM jgillan@nmsu.edu OI Duniway, Michael/0000-0002-9643-2785 FU US Dept of Agriculture National Research Initiative-Managed Ecosystems grant [2008-00776] FX This research was supported by a US Dept of Agriculture National Research Initiative-Managed Ecosystems grant to N. N. Barger (Proposal 2008-00776). NR 90 TC 0 Z9 0 U1 5 U2 19 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2016 VL 69 IS 2 BP 95 EP 107 DI 10.1016/j.rama.2015.10.012 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DG3EN UT WOS:000371952400001 ER PT J AU Jones, TA Johnson, DA Bushman, BS Connors, KJ Smith, RC AF Jones, Thomas A. Johnson, Douglas A. Bushman, B. Shaun Connors, Kevin J. Smith, Robert C. TI Seed Dormancy Mechanisms in Basalt Milkvetch and Western Prairie Clover SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE germination; prechill; rangeland legumes; rangeland plant materials; scarification; stratification ID GREAT-BASIN; ASTRAGALUS; RESTORATION; GERMINATION AB A greater diversity of native legumes and forbs is desirable for rangeland restoration practice in the Intermountain Region of the western United States. But for such diversity to materialize in the seed marketplace and to be effective in restoration practice, seeds that germinate reliably in seed fields and on restoration sites are needed. We measured germination response of two native legumes, basalt milkvetch (Astragalus filipes Torr. ex A. Gray) and western prairie clover (Dalea ornata [Douglas] Eaton & Wright), after eight germination treatments. Treatments were a factorial combination of 1) seed scarification with sandpaper (or unscarified), 2) a substrate of moist sand (or blotter paper), and 3) a 3-wk prechill at 5 degrees (or nonprechilled). Cumulative germination increased linearly throughout the 10-wk course of the experiment for all treatment combinations in both species. Scarification increased germination of western prairie clover, but prechilling and substrate had no effect. In contrast, prechilling, scarification, and a sand substrate all increased germination of basalt milkvetch. Hence, for this species the prechilled/scarified/sand treatment combination displayed the numerically highest germination for all 10 wk (30-43%), and the nonprechilled/unscarified/blotter paper treatment combination always germinated lowest (1-3%). Results were consistent with physical dormancy (hard-seededness) limiting germination of western prairie clover and combinational dormancy (i.e., co-occurrence of physical and physiological dormancy) limiting germination of basalt milkvetch. Of the two species, we have found basalt milkvetch to be the more difficult to establish from seed. By prechilling acid-scarified seed in moist sand, basalt milkvetch was successfully established in two field trials seeded in mid-April. Nonprechilled mechanically (sandpaper) scarified seed germinated as high as prechilled acid-scarified seed. By scarifying and prechilling basalt milkvetch seed to address physical and physiological dormancy mechanisms, respectively, this seed-treatment protocol may be "scaled up" to produce large quantities of germinable seed. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Jones, Thomas A.; Johnson, Douglas A.; Bushman, B. Shaun; Connors, Kevin J.; Smith, Robert C.] Utah State Univ, USDA ARS, Forage & Range Res Lab, 696 North 1100 East, Logan, UT 84322 USA. RP Jones, TA (reprint author), Utah State Univ, USDA ARS, Forage & Range Res Lab, 696 North 1100 East, Logan, UT 84322 USA. EM thomas.jones@ars.usda.gov FU USDA-ARS FX This research was funded by USDA-ARS. NR 27 TC 0 Z9 0 U1 5 U2 12 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2016 VL 69 IS 2 BP 117 EP 122 DI 10.1016/j.rama.2015.12.004 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DG3EN UT WOS:000371952400003 ER PT J AU Hardegree, SP Sheley, RL Duke, SE James, JJ Boehm, AR Flerchinger, GN AF Hardegree, Stuart P. Sheley, Roger L. Duke, Sara E. James, Jeremy J. Boehm, Alex R. Flerchinger, Gerald N. TI Temporal Variability in Microclimatic Conditions for Grass Germination and Emergence in the Sagebrush Steppe SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE model; mortality; restoration; seedbed; temperature; water availability ID FIELD-TEMPERATURE REGIMES; RESIDUE-SOIL SYSTEM; BROMUS-TECTORUM L.; GREAT-BASIN; SIMULTANEOUS HEAT; CLIMATE-CHANGE; UNITED-STATES; COLD DESERT; WATER MODEL; ECOSYSTEMS AB Sagebrush steppe ecosystems in the western United States are characterized by harsh environmental conditions with high annual and seasonal variability in both precipitation and temperature. Environmental variability contributes to widespread failure in establishing stands of desired species on degraded and invaded landscapes. To characterize seasonal microclimatic patterns and planting date effects on restoration outcomes, we evaluated long-term simulations of seed germination response of cheatgrass (Bromus tectorum L.), bottlebrush squirreltail (Elymus elymoides [Raf] Swezey), and Idaho fescue (Festuca idahoensis Elmer) to annual patterns of soil temperature and moisture. Extremely high annual variability in both the conditions favorable for germination and patterns of post-germination drought and thermal stress make it difficult to justify general inferences about seedbed treatment and planting date effects from individual, short-term field studies. We discuss the interpretation of individual-year and seasonal plant establishment factors and offer a mechanistic model for interpreting planting date and year effects on initial seedling establishment. Historical ranking and mechanistic descriptions of individual-year seedbed conditions may allow for expanded inferences through meta-analysis of limited-term field experiments. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Hardegree, Stuart P.; Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. [Sheley, Roger L.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [Duke, Sara E.] USDA ARS, Plains Area, College Stn, TX 77845 USA. [James, Jeremy J.] Sierra Foothills Res & Extens Ctr, Browns Valley, CA 95918 USA. [Boehm, Alex R.] Idaho State Univ, Dept Geosci, Boise, ID 83702 USA. RP Hardegree, SP (reprint author), USDA ARS, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. EM stuart.hardegree@ars.usda.gov FU Bureau of Land Management Intermountain Greenstripping and Rehabilitation Research Project [USDI/BLM 60-91H2-8-0020]; USDA National Institute of Food and Agriculture [AFRI-60-5360-2-832, AFRI-58-5362-3-001]; USDA-ARS Ecologically Based Invasive Plant Management program FX This work was supported by the Bureau of Land Management Intermountain Greenstripping and Rehabilitation Research Project (agreement USDI/BLM 60-91H2-8-0020), USDA National Institute of Food and Agriculture (grants AFRI-60-5360-2-832, AFRI-58-5362-3-001), and the USDA-ARS Ecologically Based Invasive Plant Management program. USDA is an equal opportunity provider and employer. NR 58 TC 2 Z9 2 U1 8 U2 22 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2016 VL 69 IS 2 BP 123 EP 128 DI 10.1016/j.rama.2015.12.002 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DG3EN UT WOS:000371952400004 ER PT J AU Sawalhah, MN Cibils, AF Maladi, A Cao, HP Vanleeuwen, DM Holechek, JL Rubio, CMB Wesley, RL Endecott, RL Mulliniks, TJ Petersen, MK AF Sawalhah, Mohammed N. Cibils, Andres F. Maladi, Aditya Cao, Huiping Vanleeuwen, Dawn M. Holechek, Jerry L. Rubio, Christina M. Black Wesley, Robert L. Endecott, Rachel L. Mulliniks, Travis J. Petersen, Mark K. TI Forage and Weather Influence Day versus Nighttime Cow Behavior and Calf Weaning Weights on Rangeland SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE global positioning system (GPS); feeding site selection; livestock; stocking rate; juniper woodland ID DAILY MAINTENANCE BEHAVIOR; RANGING SHORTHORN COWS; GPS COLLAR DATA; GRAZING DISTRIBUTION; BEEF-COWS; DISTRIBUTION PATTERNS; CHIHUAHUAN DESERT; CENTRAL AUSTRALIA; TERRAIN USE; MILK-YIELD AB We determined the effects of two forage allowance levels (LOW vs. HIGH) and weather conditions on daytime and nighttime movement patterns of young rangeland-raised cows. We also investigated whether calf weaning weights (n = 42) were significantly related to postcalving movement patterns of the dam. Global positioning system data were collected over 4 years by recording 5-min interval locations of 52 crossbred cows grazing a 146-ha woodland/grassland pasture for approximately 20 days. The pasture was stocked moderately in 2004 (73 AUMs) and 2005 (78 AUMs) and lightly in 2006 (34 AUMs) and 2007 (32 AUMs). Estimated forage allowance was low in 2004 and 2005 (347 and 438 kg herbage.cow(-1), respectively) and high in 2006 and 2007 (1104 and 1884 kg herbage.cow(-1), respectively). We calculated distance traveled, path sinuosity, woodland preference, and area explored for each cow during 24 h (D + N), daytime (DAY), and nighttime (PRE dawn and POST sunset) periods. Cows in LOW traveled farther than counterparts in HIGH during D + N and DAY (P < 0.01) periods but traveled shorter or similar distances during POST (P = 0.05) and PRE (P = 0.29) nighttime periods, respectively. Cows in LOW exhibited more sinuous movement paths than cows in HIGH during DAY, PRE, and POST periods (P 5 0.01). Cows in LOW explored larger areas and spent more time in woodlands than counterparts in HIGH (P < 0.01). Weather factors associated with thermal comfort affected daily variation in both daytime and nighttime movement patterns of cows. A dam's movement patterns in the weeks immediately following calving were correlated (P <= 0.01) with steer but not heifer calf WW.Moderate stocking rates (LOW treatment) induced behaviors that resulted in higher woodland preference and heavier steer calf WW. (C) 2016 The Society for Range Management. Published by Elsevier Inc. All rights reserved. C1 [Sawalhah, Mohammed N.; Rubio, Christina M. Black] Hashemite Univ, Dept Nat Resources, POB 330127, Zarqa 13133, Jordan. [Cibils, Andres F.; Holechek, Jerry L.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. [Maladi, Aditya; Cao, Huiping] New Mexico State Univ, Dept Comp Sci, Las Cruces, NM 88003 USA. [Vanleeuwen, Dawn M.] New Mexico State Univ, Dept Econ Appl Stat & Int Business, Las Cruces, NM 88003 USA. [Wesley, Robert L.] SRP McGinley Ranch, Gordon, NE 69343 USA. [Endecott, Rachel L.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USA. [Mulliniks, Travis J.] Univ Tennessee, Dept Anim Sci, Crossville, TN 38571 USA. [Petersen, Mark K.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Cibils, AF (reprint author), New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. EM acibils@nmsu.edu FU U.S. Dept of Agriculture National Institute of Food and Agriculture, Hatch project [1000985]; New Mexico Agricultural Experiment Station; New Mexico State University Corona Range and Livestock Research Center; U.S. Dept of Agriculture-ARS Jornada Experimental Range FX This research was funded by the U.S. Dept of Agriculture National Institute of Food and Agriculture, Hatch project 1000985. Partial support was provided by the New Mexico Agricultural Experiment Station, the New Mexico State University Corona Range and Livestock Research Center, and the U.S. Dept of Agriculture-ARS Jornada Experimental Range. NR 67 TC 0 Z9 0 U1 4 U2 11 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2016 VL 69 IS 2 BP 134 EP 143 DI 10.1016/j.rama.2015.10.007 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DG3EN UT WOS:000371952400006 ER PT J AU Branson, DH Vermeire, LT AF Branson, David H. Vermeire, Lance T. TI Grasshopper Responses to Fire and Postfire Grazing in the Northern Great Plains Vary Among Species SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE drought; grasshopper; grassland; herbivory; livestock grazing; prescribed burn ID MIXED-GRASS PRAIRIE; TALLGRASS PRAIRIE; RANGELAND GRASSHOPPERS; INSECT HERBIVORY; FOOD LIMITATION; EGG MORTALITY; SUMMER FIRE; ACRIDIDAE; ORTHOPTERA; ASSEMBLAGES AB Rangeland management practices such as burning and grazing may affect the development, survival, and reproduction of grasshopper populations. Experiments in the northern Great Plains that examine effects of fire and grazing utilization on grasshoppers are lacking. As part of a larger study examining vegetation responses to late summer fire and postfire grazing utilization in semiarid mixed prairie in eastern Montana to aid in postfire management decisions, we examined grasshopper responses to late summer fire and postfire grazing intensity. The experiment was repeated using adjacent blocks, with blocks receiving fire treatment in either 2003 or 2004 and grazing in the following year. Treatments were no fire and no grazing, and summer fire followed by grazing at 0%, 17%, or 50% forage utilization on a biomass basis. Grasshopper sampling was conducted before fire and for 2 years post fire. Fire reduced grasshopper density 36-53% across experiments, sampling periods, and postfire grazing treatments, but the effects of grazing and fire were species dependent. The two most abundant grasshopper species, Ageneotettix deorum (Scudder) and Opeia obscura (Thomas), were reduced 80% and 84% the first year after the 2003 fire, but only O. obscura was affected following the 2004 fire. Late summer fire appears to be a useful management tool to reduce populations of some grasshopper species in the northern Great Plains, while other species appear more responsive to food limitation from increased postfire grazing utilization. Fire effects were largely driven by two species, indicating that late-season fire impacts could be species dependent. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Branson, David H.] ARS, USDA, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. [Vermeire, Lance T.] ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Branson, DH (reprint author), ARS, USDA, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM dave.branson@ars.usda.gov NR 34 TC 0 Z9 0 U1 7 U2 15 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAR PY 2016 VL 69 IS 2 BP 144 EP 149 DI 10.1016/j.rama.2015.10.005 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DG3EN UT WOS:000371952400007 ER PT J AU Mahmoud, UT Cheng, HW Applegate, TJ AF Mahmoud, U. T. Cheng, H. W. Applegate, T. J. TI Functions of propolis as a natural feed additive in poultry SO WORLDS POULTRY SCIENCE JOURNAL LA English DT Article DE propolis; poultry; performance; oxidative damage; physiology ID DIETARY TURKISH PROPOLIS; COTURNIX-COTURNIX-JAPONICA; LAYING HENS; BROILER-CHICKENS; HEAT-STRESS; CARCASS CHARACTERISTICS; EGG QUALITY; BEE POLLEN; VITAMIN-C; PERFORMANCE AB Propolis is a resinous hive product collected by honeybees from various sources of plants. Numerous scientific investigations have been focused on the biological activities of propolis and its functions as a health supplement in humans. It could have similar function in other animals, such as in poultry. This review is focused on the recent findings concerning the effects of propolis, as an antioxidant, on performance, carcass characteristics, behaviour, immunity, and physiological homeostasis in domesticated poultry species (broiler chickens, laying hens, quail, and duck); and the needs in future poultry research. C1 [Mahmoud, U. T.] Univ Assiut, Fac Vet Med, Dept Anim Hyg, Assiut 71526, Egypt. [Cheng, H. W.] Purdue Univ, USDA ARS, 125 S Russell St, W Lafayette, IN 47907 USA. [Applegate, T. J.] Purdue Univ, Dept Anim Sci, 915 West State St, W Lafayette, IN 47907 USA. RP Mahmoud, UT (reprint author), Univ Assiut, Fac Vet Med, Dept Anim Hyg, Assiut 71526, Egypt. EM usamakomsan5@gmail.com OI mahmoud, usama/0000-0001-8650-2420 NR 65 TC 0 Z9 0 U1 7 U2 17 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0043-9339 EI 1743-4777 J9 WORLD POULTRY SCI J JI Worlds Poult. Sci. J. PD MAR PY 2016 VL 72 IS 1 BP 37 EP 47 DI 10.1017/S0043933915002731 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DG7KM UT WOS:000372263200003 ER PT J AU Liu, ZS Chen, J Knothe, G Nie, XA Jiang, JC AF Liu, Zengshe Chen, Jie Knothe, Gerhard Nie, Xiaoan Jiang, Jianchun TI Synthesis of Epoxidized Cardanol and Its Antioxidative Properties for Vegetable Oils and Biodiesel SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Vegetable oil; Biodiesel; Oxidative stability; Antioxidants; Epoxidized cardanol ID STABILITY; OXIDATION; ACIDS; DERIVATIVES; CHLORIDE; STORAGE; FOODS AB A novel antioxidant epoxidized cardanol (ECD), derived from cardanol, was synthesized and characterized by FT-IR, H-1 NMR, and C-13 NMR Oxidative stability of ECD used in vegetable oils and biodiesel was evaluated by pressurized differential scanning calorimetry (PDSC) and the Rancimat method, respectively. The results indicated that ECD exhibited antioxidative activity in soybean oil and increased its onset temperature (OT) by nearly 10 degrees C with 0.7 wt % ECD. It was also observed that other vegetables oils showed significantly improved oxidative stability with the addition of 0.7 wt % ECD. Olive oil showed the highest increased OT by 19.5 degrees C. Furthermore, ECD has superior antioxidant activity compared to synthetic antioxidant butylated hydroxytoluene (BHT) and thus could be used as an optimized primary antioxidant for biodiesel. Thermogravimetric analysis (TGA) indicated that ECD shows better thermal stability than cardanol. The data presented in this study indicate that ECD could be a new biobased antioxidant with better thermal stability. C1 [Liu, Zengshe; Knothe, Gerhard] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Chen, Jie; Nie, Xiaoan; Jiang, Jianchun] Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Natl Engn Lab Biomass Chem Utilizat, Key Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China. RP Liu, ZS (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM kevin.liu@ars.usda.gov FU National "Twelfth Five-Year" Plan for Science & Technology Support [2015BAD15B08]; Basic research funding earmarked for the national commonweal research institutes, CAF [CAFINT2014C12] FX Jie Chen is grateful to the financial support from National "Twelfth Five-Year" Plan for Science & Technology Support (Grant number: 2015BAD15B08) and Basic research funding earmarked for the national commonweal research institutes, CAF (Grant number: CAFINT2014C12). The authors also gratefully acknowledge Daniel Knetzer for help in PDSC analysis and Kevin Steidley for help in Rancimat test. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 30 TC 2 Z9 2 U1 1 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD MAR PY 2016 VL 4 IS 3 BP 901 EP 906 DI 10.1021/acssuschemeng.5b00991 PG 6 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DG0LD UT WOS:000371755400034 ER PT J AU Tarves, PC Mullen, CA Boateng, AA AF Tarves, Paul C. Mullen, Charles A. Boateng, Akwasi A. TI Effects of Various Reactive Gas Atmospheres on the Properties of Bio-Oils Produced Using Microwave Pyrolysis SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Microwave; Pyrolysis; Lignocellulosic biomass; Bio-oil; Deoxygenation ID CATALYTIC FAST PYROLYSIS; BIOMASS; ZEOLITES AB Fast pyrolysis of lignocellulosic biomass produces organic liquids (bio-oil), biochar, water, and noncondensable gases. The noncondensable gas component typically contains syngas (H-2, CO, and CO2) as well as small hydrocarbons (CH4, C2H6, and C3H8). To understand the influence of reactive gas in various pyrolysis processes, we have employed a laboratory scale microwave reactor and performed pyrolysis of switchgrass under varying gaseous atmospheres and characterized the bio-oils obtained. The batch (100 g of biomass) microwave pyrolysis was performed at 900-1000 W over the course of 7 min in the presence of a microwave absorber (10 g of activated charcoal). The products formed were quantified and the bio-oils were characterized by GC-MS, elemental analysis, Karl Fischer and TAN titrations, bomb calorimetry, and C-13 NMR spectroscopy. Pyrolysis experiments performed under a N-2 atmosphere were used as the control and then compared to experiments performed under various reactive gases (CO, H-2, and CH4) and a model pyrolysis gas mixture ("PyGas"). The use of a CO atmosphere had a negligible effect on the quantity and quality of bio-oils produced, whereas the use of H-2, CH4, and PyGas atmospheres each provided more deoxygenated products (i.e., BTEX, naphthalenes, etc.) and lower oxygen content. The use of different particle sizes also displayed a pronounced effect on the product distribution and the composition of the bio-oils obtained. C1 [Tarves, Paul C.; Mullen, Charles A.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Mullen, CA (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov FU USDANIFA-BRDI Grant [2012-10008-20271] FX The authors thank Dr. Gary Strahan for NMR experiments. We also thank Patrick West and Jennifer Gallup, ERRC co-op students from Drexel University for technical assistance. Funding from USDANIFA-BRDI Grant No. 2012-10008-20271 is hereby acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 18 TC 3 Z9 3 U1 6 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD MAR PY 2016 VL 4 IS 3 BP 930 EP 936 DI 10.1021/acssuschemeng.5b01016 PG 7 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DG0LD UT WOS:000371755400037 ER PT J AU Reza, MT Yang, XK Coronella, CJ Lin, HF Hathwaik, U Shintani, D Neupane, BP Miller, GC AF Reza, M. Toufiq Yang, Xiaokun Coronella, Charles J. Lin, Hongfei Hathwaik, Upul Shintani, David Neupane, Bishnu P. Miller, Glenn C. TI Hydrothermal Carbonization (HTC) and Pelletization of Two Arid Land Plants Bagasse for Energy Densification SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Hydrothermal carbonization; Gumweed; Rabbitbrush; Bagasse; Hydrochar; Ultimate analysis ID LIGNOCELLULOSIC BIOMASS; BIOCHAR; SOUTHWEST; CARBON; WASTE; ACID AB In this study, bagasse from two arid land plants, grindelia and rabbitbrush, were hydrothermally carbonized (HTC) along with their raw biomass at 200-260 degrees C for 5 min. Prior to HTC, biocrude was extracted from grindelia (Grindelia squarrosa), whereas rubber was extracted from rabbitbrush (Ericameria nauseosa). Solid hydrochars and HTC process liquids of extracted feedstocks were characterized by ultimate, proximate, fiber, FTIR, higher heating value (HHV), and GC-MS analyses and the results were compared with their corresponding unextracted conditions. Hydrochars were pelletized in a single-press pelletizer and mass and energy densities of the pellets were measured. From the proximate, ultimate, FTIR, and fiber analyses, the bagasse show similar properties of the raw biomass, although the HHV was slightly increased with crude extraction from grindelia and decreased with rubber extraction from rabbitbrush. With the increase of HTC temperature, solid mass yield was decreased up to 44% for grindelia bagasse and 57% for rabbitbrush bagasse. HHV increases for all the feedstocks up to about 26 MJ kg(-1), regardless of biomass type, when treated at 260 degrees C. HTC process liquid becomes acidic in the presence of short-chain organic acids with HTC temperature. C1 [Reza, M. Toufiq; Yang, Xiaokun; Coronella, Charles J.; Lin, Hongfei] Univ Nevada, Dept Chem & Mat Engn, 1664 N Virginia St, Reno, NV 89557 USA. [Hathwaik, Upul] ARS, Bioprod Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. [Shintani, David] Univ Nevada, Dept Mol Biosci, 1664 N Virginia St, Reno, NV 89557 USA. [Neupane, Bishnu P.; Miller, Glenn C.] Univ Nevada, Dept Nat Resource & Environm Sci, 1664 N Virginia St, Reno, NV 89557 USA. RP Reza, MT (reprint author), Univ Nevada, Dept Chem & Mat Engn, 1664 N Virginia St, Reno, NV 89557 USA. EM mreza@unr.edu OI Reza, M. Toufiq/0000-0001-9856-5947 FU USDA-NIFA [11322628] FX This research is supported by USDA-NIFA (grant no. 11322628). The authors acknowledge Drs. Teshome Senkuru, Simon Paulson, Stephen Spain, and Mr. Keenan Conrad from University of Nevada, Reno for their support in experimental and analytical tasks. NR 38 TC 5 Z9 5 U1 6 U2 38 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD MAR PY 2016 VL 4 IS 3 BP 1106 EP 1114 DI 10.1021/acssuschemeng.5b01176 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DG0LD UT WOS:000371755400058 ER PT J AU Bigornia, SJ Lichtenstein, AH Harris, WS Tucker, KL AF Bigornia, Sherman J. Lichtenstein, Alice H. Harris, William S. Tucker, Katherine L. TI Associations of erythrocyte fatty acid patterns with insulin resistance SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE fatty acids; insulin resistance; principal components analysis; Boston Puerto Rican Health Study; diet ID HOMEOSTASIS MODEL ASSESSMENT; FOOD-FREQUENCY QUESTIONNAIRE; CORONARY-HEART-DISEASE; HISPANIC WHITE ELDERS; PUERTO-RICAN ADULTS; DE-NOVO LIPOGENESIS; CARDIOVASCULAR-DISEASE; METABOLIC SYNDROME; PHYSICAL-ACTIVITY; CARIBBEAN ORIGIN AB Background: Synergistic or additive effects or both on cardiometabolic risk may be missed by examining individual fatty acids (FAs). A pattern analysis may be a more useful approach. In addition, it remains unclear whether erythrocyte FA composition relates to insulin resistance among Hispanics/Latinos. Objective: We derived erythrocyte FA patterns for a Puerto Rican cohort and examined their association with diet and insulin resistance in cross-sectional and prospective analyses. Design: At baseline, principal components analysis was used to derive factor patterns with the use of 24 erythrocyte FAs from 1157 participants of the Boston Puerto Rican Health Study (aged 45-75 y). Dietary intake was assessed with a validated semiquantitative food-frequency questionnaire. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated at baseline and at the 2-y follow-up. Relations between FA patterns and HOMA-IR were analyzed in a sample of 922 participants with available data. Results: Five FA patterns were derived, differentiated by 1) relatively high de novo lipogenesis (DNL) FAs and low n-6 (4)-6) FAs, 2) high very-long-chain saturated FAs, 3) high n-3 (omega-3) FAs, 4) high linoleic acid and low arachidonic acid, and 5) high trans FAs. The DNL pattern was positively correlated with sugar and inversely with n-6 and monounsaturated FA intakes. Only the DNL pattern was positively related to baseline HOMA-TR. [adjusted geometric means (95% CIs) for quartiles 1 and 4: 1.72 (1.58, 1.87) and 2.20 (2.02, 2.39); P-trend < 0.0001]. Similar associations were observed at 2 y, after adjustment for baseline status [quartiles 1 and 4 means (95% CIs): 1.61 (1.48, 1.76) and 1.84. (1.69, 2.00); P-trend = 0.02]. These results remained consistent after the exclusion of participants with diabetes (n = 485). Conclusion: Our findings suggest that upregulated DNL associated with a diet high in sugar and relatively low in unsaturated FAs may adversely affect insulin sensitivity in a Hispanic/Latino cohort. C1 [Bigornia, Sherman J.; Tucker, Katherine L.] Univ Massachusetts Lowell, Clin Lab & Nutr Sci, Lowell, MA USA. [Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Harris, William S.] Univ S Dakota, Sanford Sch Med, Dept Med, Sioux Falls, SD USA. RP Bigornia, SJ (reprint author), Univ Massachusetts Lowell, Clin Lab & Nutr Sci, Lowell, MA USA. EM bigornia@bu.edu FU NIH [P01-AG023394, P50-HL105185, R01-AG027087] FX Supported by NIH grants P01-AG023394, P50-HL105185, and R01-AG027087. The Health Diagnostic Laboratory, Inc. (Richmond, Virginia) performed the fatty acid assays free of charge. NR 49 TC 1 Z9 1 U1 2 U2 13 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 2016 VL 103 IS 3 BP 902 EP 909 DI 10.3945/ajcn.115.123604 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DF8ZO UT WOS:000371650200028 PM 26864364 ER PT J AU Barnett, JB Dao, MC Hamer, DH Kandel, R Brandeis, G Wu, DY Dallal, GE Jacques, PF Schreiber, R Kong, E Meydani, SN AF Barnett, Junaidah B. Dao, Maria C. Hamer, Davidson H. Kandel, Ruth Brandeis, Gary Wu, Dayong Dallal, Gerard E. Jacques, Paul F. Schreiber, Robert Kong, Eunhee Meydani, Simin N. TI Effect of zinc supplementation on serum zinc concentration and T cell proliferation in nursing home elderly: a randomized, double-blind, placebo-controlled trial SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE T cell proliferation; nursing home elderly; serum zinc concentration; zinc gluconate; zinc supplementation ID ATOMIC-ABSORPTION SPECTROSCOPY; CYTOKINE PRODUCTION; IMMUNE-RESPONSE; MONONUCLEAR-CELLS; DIETARY ZINC; DEFICIENCY; INFECTIONS; HOMEOSTASIS; PNEUMONIA; POPULATION AB Background: Zinc is essential for the regulation of immune response. T cell function declines with age. Zinc supplementation has the potential to improve the serum zinc concentrations and immunity of nursing home elderly with a low serum zinc concentration. Objective: We aimed to determine the effect of supplementation with 30 mg Zn/d for 3 mo on serum zinc concentrations of zinc deficient nursing home elderly. Design: This was a randomized, double-blind, placebo-controlled study. Of 53 nursing home elderly (aged >= 65 y) who met eligibility criteria, 58% had a low serum zinc concentration (serum zinc <70 mu g/dL); these 31 were randomly assigned to zinc (30 mg Zn/d) (n = 16) or placebo (5 mg Zn/d) (n = 15) groups. The primary outcome measure was change in serum zinc concentrations between baseline and month 3. We also explored the effects of supplementation on immune response. Results: Baseline characteristics were similar in the 2 groups. The difference in the mean change in serum zinc was significantly higher, by 16%, in the zinc group than in the placebo group (P = 0.007) when baseline zinc concentrations were controlled for. In addition, controlling for baseline C-reactive protein, copper, or albumin did not change the results. However, supplementation of participants with <= 60 mu g serum Zn/dL failed to increase their serum zinc to >= 70 mu g/dL. Zinc supplementation also significantly increased anti-CD3/CD28 and phytohemagglutinin-stimulated T cell proliferation, and the number of peripheral T cells (P < 0.05). When proliferation was expressed per number of T cells, the significant differences between groups were lost, suggesting that the zinc-induced enhancement of T cell proliferation was mainly due to an increase in the number of T cells. Conclusions: Zinc supplementation at 30 mg/d for 3 mo is effective in increasing serum zinc concentrations in nursing home elderly; however, not all zinc-deficient elderly reached adequate concentrations. The increase in serum zinc concentration was associated with the enhancement of T cell function mainly because of an increase in the number of T cells. C1 [Barnett, Junaidah B.; Dao, Maria C.; Hamer, Davidson H.; Wu, Dayong; Dallal, Gerard E.; Jacques, Paul F.; Kong, Eunhee; Meydani, Simin N.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. [Hamer, Davidson H.] Boston Univ, Sch Med, Sect Infect Dis, Boston, MA 02118 USA. [Brandeis, Gary] Boston Univ, Sch Med, Dept Med, Sect Geriatr, Boston, MA 02118 USA. [Hamer, Davidson H.] Boston Univ, Sch Publ Hlth, Ctr Global Hlth & Dev, Boston, MA USA. [Hamer, Davidson H.] Boston Univ, Sch Publ Hlth, Dept Global Hlth, Boston, MA USA. [Kandel, Ruth; Schreiber, Robert] Hebrew SeniorLife, Roslindale, MA USA. [Kandel, Ruth; Schreiber, Robert] Harvard Univ, Sch Med, Boston, MA USA. RP Meydani, SN (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. EM simin.meydani@tufts.edu FU USDA Agricultural Research Service [58-1950-0-014] FX This material is based on work supported by the USDA Agricultural Research Service under agreement no. 58-1950-0-014. This work was performed at the Nutritional Immunology Laboratory, Jean Mayer USDA Human Nutrition Research Center at Tufts University, Boston, MA. NR 63 TC 2 Z9 2 U1 5 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAR PY 2016 VL 103 IS 3 BP 942 EP 951 DI 10.3945/ajcn.115.115188 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DF8ZO UT WOS:000371650200033 PM 26817502 ER PT J AU Bosco-Lauth, AM Nemeth, NM Kohler, DJ Bowen, RA AF Bosco-Lauth, Angela M. Nemeth, Nicole M. Kohler, Dennis J. Bowen, Richard A. TI Viremia in North American Mammals and Birds after Experimental Infection with Chikungunya Viruses SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID INDIAN-OCEAN; AEDES-ALBOPICTUS; VECTORS; SPREAD; ANTIBODIES; OUTBREAKS; SENEGAL; AEGYPTI; DISEASE AB Chikungunya virus (CHIKV) is an arthropod-borne virus, which is known to cause severe disease only in humans. To investigate its potential zoonotic host range and evaluate reservoir competence among these hosts, experimental infections were performed on individuals from nine avian and 12 mammalian species representing both domestic and wild animals common to North America. Hamsters and inbred mice have previously been shown to develop viremia after inoculation with CHIKV and were used as positive controls for infection. Aside from big brown bats (Eptesicus fuscus), none of the mammals or birds developed detectable viremia or overt clinical disease. However, most mammals and a smaller proportion of birds developed neutralizing antibody responses to CHIKV. On the basis of these results, it seems unlikely that CHIKV poses a significant health threat to most domestic animals or wildlife and that the species examined do not likely contribute to natural transmission cycles. Additional studies should further evaluate bats and wild rodents as potential reservoir hosts for CHIKV transmission during human epidemics. C1 [Bosco-Lauth, Angela M.; Bowen, Richard A.] Colorado State Univ, Dept Biomed Sci, Ft Collins, CO 80523 USA. [Nemeth, Nicole M.] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Dis Program, Ft Collins, CO USA. [Kohler, Dennis J.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Ft Collins, CO USA. RP Bowen, RA (reprint author), Colorado State Univ, Dept Biomed Sci, Anim Reprod & Biotechnol Lab, Foothills Campus, Ft Collins, CO 80523 USA. EM mopargal@rams.colostate.edu; nnemeth@uoguelph.ca; dennis.kohler@aphis.usda.gov; rbowen@colostate.edu NR 22 TC 0 Z9 0 U1 4 U2 14 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA SN 0002-9637 EI 1476-1645 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD MAR PY 2016 VL 94 IS 3 BP 504 EP 506 DI 10.4269/ajtmh.15-0696 PG 3 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA DF5DS UT WOS:000371372600006 PM 26666699 ER PT J AU Machtinger, ET Geden, CJ LoVullo, ED Shirk, PD AF Machtinger, E. T. Geden, C. J. LoVullo, E. D. Shirk, P. D. TI Impacts of Extended Laboratory Rearing on Female Fitness in Florida Colonies of the Parasitoid Spalangia cameroni (Hymenoptera: Pteromalidae) With an Analysis of Wolbachia Strains SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE biological control; Musca domestica; Stomoxys calcitrans; filth fly; parasitoid ID MUSCIDIFURAX-RAPTOR HYMENOPTERA; HOUSE-FLIES; INSECTICIDE RESISTANCE; INBREEDING DEPRESSION; GENETIC ADAPTATION; SURFACE PROTEIN; MUSCOID FLIES; HOST LOCATION; DIPTERA; MUSCIDAE AB Spalangia cameroni Perkins is used as a biological control agent of filth flies. These parasitoids are reared commercially, but little is known about the impact of colony age on host-seeking and life history parameters. Hostseeking in bedding from equine stalls (pine shavings and manure) was analyzed with two colonies, one established in 2000 and one in 2010 (144 and 24 generations in colony). These two colonies, and a colony established in 2012 colony (4 generations), were analyzed for differences in female longevity and fecundity. Wolbachia were analyzed in each colony to document sequence variants. All wasps collected to start parasitoid colonies were from the same dairy in Gilchrist County, FL, but collected on different dates. Parasitoids from the 2000 colony parasitized fewer hosts than ones from the 2010 colony when challenged in a complex environment. Life history parameters were different between individuals in the colonies. Differences in reproductive productivity between the colonies could not be correlated to the presence or sequence variant of Wolbachia. New Wolbachia wsp and ftsZ sequences were identified in these colonies. Multiple strains of Wolbachia were found in each colony, and their presence did not vary among colonies. The invariance in strain presence suggests that Wolbachia strains were not lost during colony maintenance, i.e., lost through selection or random genetic drift. C1 [Machtinger, E. T.] Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. [Geden, C. J.; LoVullo, E. D.; Shirk, P. D.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 S-W 23rd Dr, Gainesville, FL 32608 USA. RP Machtinger, ET (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. EM irishtangerine@ufl.edu; chris.geden@ars.usda.gov; Eric.LoVullo@ARS.USDA.GOV; paul.shirk@ars.usda.gov FU USDA Southern Sustainable Agriculture and Research Education (SARE) award [GS-11-101] FX We would like to thank Dana Johnson for her extensive help with the molecular work and maintaining laboratory fly colonies. We would also like to thank Neil Sanscraint for screening the parasitoid colonies for Nosema. The reviews of the content of this manuscript by Dr. Heather McAuslane, Dr. Hans Alborn, and Dr. Amanda House are appreciated. We would like to thank Dr. Peter Teal for his inspiration and guidance. This research was supported by a USDA Southern Sustainable Agriculture and Research Education (SARE) award GS-11-101. NR 47 TC 0 Z9 0 U1 9 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2016 VL 109 IS 2 BP 176 EP 182 DI 10.1093/aesa/sav118 PG 7 WC Entomology SC Entomology GA DG2OF UT WOS:000371906100003 ER PT J AU Lucini, T Panizzi, AR Backus, EA AF Lucini, Tiago Panizzi, Antonio Ricardo Backus, Elaine A. TI Characterization of an EPG Waveform Library for Redbanded Stink Bug, Piezodorus guildinii (Hemiptera: Pentatomidae), on Soybean Plants SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Heteroptera; Pentatomidae; electrical penetration graph; electropenetrography; electronic feeding monitor ID ELECTRICAL PENETRATION GRAPHS; STYLET PENETRATION; HESPERUS HEMIPTERA; XYLELLA-FASTIDIOSA; FEEDING-BEHAVIOR; HETEROPTERA; APHIDS; DAMAGE; TRANSMISSION; CICADELLIDAE AB Feeding behaviors of the redbanded stink bug, Piezodorus guildinii (Westwood), on vegetative (stem and leaflet) and reproductive (pod) tissues of soybean, Glycine max (L.), were recorded using an AC-DC electropenetrograph (EPG) apparatus. Eight different probing waveforms were characterized and defined: Pg1a, Pg1b, Pg1c, Pg1d, Pg2, Pg3a, Pg3b, and Pg4 grouped into three different families, P, I, and N. Histological studies of intact stylets within salivary sheaths observed during Pg1b, Pg1c, Pg2, and Pg3 waveforms were correlated with the specific penetration sites. Waveforms Pg1a, Pg1b, Pg1c, and Pg1d (pathway-family P) occurred at the start of probing activities and represent stylet penetration deep into plant tissue. Waveforms Pg2, Pg3a, and Pg3b (family I) represent the food ingestion phase. Pg2 waveform represents xylem sap ingestion primarily on leaves and stems. During Pg3a, stylets were moving, lacerating deeply into pod tissue and partially retracting; during Pg3b, stylets were motionless inside the pod tissue. Pg3b occurred interspersed with waveform Pg3a. Waveform Pg4 (family N) represented short interruptions that occurred within waveform Pg2. The study demonstrated that P. guildinii uses the cell rupture strategy to ingest from endosperm in soybean pod, and the same insect could switch to salivary sheath feeding to ingest from xylem in soybean leaves and stems. This unusual behavior explains symptoms of stink bug damage to soybean. The P. guildinii waveforms defined herein will allow future EPG studies to aid in development of soybean varieties that resist the feeding and damage caused by this and other stink bug pests. C1 [Lucini, Tiago] Univ Fed Parana, Dept Zool, POB 19020, BR-81531980 Curitiba, PR, Brazil. [Panizzi, Antonio Ricardo] Embrapa Wheat, Entomol Lab, POB 3081, BR-99001970 Passo Fundo, RS, Brazil. [Backus, Elaine A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Panizzi, AR (reprint author), Embrapa Wheat, Entomol Lab, POB 3081, BR-99001970 Passo Fundo, RS, Brazil. EM tiago_lucini@hotmail.com; antonio.panizzi@embrapa.br; elaine.backus@ars.usda.gov FU National Council of Research and Technology of Brazil (CNPq) [471517/2012-7]; CAPES (Ministry of Education) of Brazil; Embrapa Unit at Passo Fundo, RS FX This study was partially supported by a National Council of Research and Technology of Brazil (CNPq) grant 471517/2012-7 to A. R. P., and by a scholarship from CAPES (Ministry of Education) of Brazil to T. L. in order to obtain the Doctor of Science (Entomology) degree at the Federal University of Parana at Curitiba, Brazil. We also thank the Embrapa Unit at Passo Fundo, RS, for support. NR 34 TC 1 Z9 1 U1 3 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2016 VL 109 IS 2 BP 198 EP 210 DI 10.1093/aesa/sav156 PG 13 WC Entomology SC Entomology GA DG2OF UT WOS:000371906100005 ER PT J AU Horton, DR Lewis, TM Garczynski, SF Thomsen-Archer, K Unruh, TR AF Horton, David R. Lewis, Tamera M. Garczynski, Stephen F. Thomsen-Archer, Kelly Unruh, Thomas R. TI Morphological and Genetic Reappraisal of the Orius Fauna of the Western United States (Hemiptera: Heteroptera: Anthocoridae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Orius; genitalia; mtDNA; cryptic species ID PHYLOGENETIC UTILITY; BIOLOGICAL-CONTROL; GENUS ORIUS; NORTH-AMERICA; BUGS; INSIDIOSUS; ORGANISMS; SEQUENCES; IDENTITY; PRIMERS AB Examination of minute pirate bugs, Orius spp. (Hemiptera: Heteroptera: Anthocoridae), from a broad geographic range in the western United States prompted a reappraisal of the taxonomic composition of the fauna native to the western United States and Canada. Current syntheses and catalogs list three species of Orius native to this region. In a previous study, we showed how geographic variation in external traits of one of these species, Orius diespeter Herring, 1966, had led to mistakes in identification of species within this complex. More extensive collecting efforts have now led to the discovery of specimens having traits not fully consistent with descriptions of any described species. We provisionally categorized these unresolved specimens into one of eight phenotypic groups based upon combinations of body size, visual appearance of genitalia, association with specific plant taxa, and geographic source. Genitalia from 382 specimens were then measured to determine whether phenotypic groupings were confirmed by statistical analysis of genitalic morphology. Principal components analysis showed that size and shape of the male's paramere differed among phenotypes. The paramere of unresolved specimens often diverged from the paramere of described species. Length of the female's copulatory tube differed between several of the unresolved phenotypes and described species. Analysis of DNA sequences showed that five of the eight phenotypes diverged genetically from other phenotypes and from described species. DNA sequence data did not separate two described species (Orius tristicolor (White, 1879) and Orius harpocrates Herring, 1966), suggesting that these two species are a single species. The combined morphological and genetic evidence indicates that the Orius fauna of the western United States is composed of a mix of two described species and possibly five undescribed cryptic species. We summarize the known distributions of described and cryptic undescribed species, and discuss the implications of our work for the biological control community. C1 [Horton, David R.; Lewis, Tamera M.; Garczynski, Stephen F.; Thomsen-Archer, Kelly; Unruh, Thomas R.] USDA ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Horton, DR (reprint author), USDA ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM david.horton@ars.usda.gov; tamera.lewis@ars.usda.gov; steve.garczynski@ars.usda.gov; kelly.thomsenarcher@ars.usda.gov; thomas.unruh@ars.usda.gov NR 63 TC 0 Z9 0 U1 2 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAR PY 2016 VL 109 IS 2 BP 319 EP 334 DI 10.1093/aesa/sav155 PG 16 WC Entomology SC Entomology GA DG2OF UT WOS:000371906100013 ER PT J AU Ball, EV Wang, SL Nehring, R Mosheim, R AF Ball, Eldon V. Wang, Sun L. Nehring, Richard Mosheim, Roberto TI Productivity and Economic Growth in US Agriculture: A New Look(aEuro) SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Agriculture; total factor productivity; production accounts AB It is widely reported that productivity growth is the main contributor to economic growth in U.S. agriculture. This article provides estimates of economic growth since World War II and decomposes that growth into the contributions of input growth and productivity growth. The analysis is based on recently revised production accounts, now spanning the 1948-2013 period. Our findings are fully consistent with those reported in the literature; productivity growth dominates input growth as a source of economic growth in the agricultural sector. C1 [Ball, Eldon V.; Wang, Sun L.; Nehring, Richard; Mosheim, Roberto] USDA, Econ Res Serv, Washington, DC 20024 USA. RP Ball, EV (reprint author), USDA, Econ Res Serv, Washington, DC 20024 USA. EM eball@ers.usda.gov NR 22 TC 0 Z9 0 U1 1 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2016 VL 38 IS 1 BP 30 EP 49 DI 10.1093/aepp/ppv031 PG 20 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DF9KB UT WOS:000371678700002 ER PT J AU Zahniser, S Hertz, T Argoti, M AF Zahniser, Steven Hertz, Tom Argoti, Monica TI Quantifying the Effects of Mexico's Retaliatory Tariffs on Selected US Agricultural Exports SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Trade; Mexico; United States; retaliatory tariffs; trucking dispute; NAFTA AB This article estimates the trade-reducing effects of the retaliatory import tariffs imposed by Mexico on selected U.S. agricultural products from March 2009 to October 2011 as part of the U.S.-Mexico trucking dispute. Using an autoregressive distributed-lag time series model of the targeted agricultural exports, we find that the tariffs reduced U.S. sales of these products to Mexico by $984 million (22%). We find no evidence that reduced exports to Mexico were offset by increased sales of these same goods to other countries. The large impact of the tariffs underscores the importance of the duty-free provisions of the North American Free Trade Agreement, as well as the potentially high costs of retaliatory trade measures. C1 [Zahniser, Steven] USDA, Econ Res Serv, Agr Economist, Washington, DC 20024 USA. [Hertz, Tom] USDA, Econ Res Serv, Economist, Washington, DC 20024 USA. [Argoti, Monica] USDA, Anim & Plant Hlth Inspect Serv, Economist, Washington, DC USA. [Argoti, Monica] HACU, Natl Internship Program, Washington, DC USA. RP Zahniser, S (reprint author), USDA, Econ Res Serv, Agr Economist, Washington, DC 20024 USA. EM zahniser@ers.usda.gov NR 30 TC 0 Z9 0 U1 3 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2016 VL 38 IS 1 BP 93 EP 112 DI 10.1093/aepp/ppv008 PG 20 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DF9KB UT WOS:000371678700005 ER PT J AU Haim, D White, EM Alig, RJ AF Haim, David White, Eric M. Alig, Ralph J. TI Agriculture Afforestation for Carbon Sequestration Under Carbon Markets in the United States: Leakage Behavior from Regional Allowance Programs SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Agricultural afforestation; carbon markets; economics; leakage ID MITIGATION; EMISSIONS; SINKS; US AB This study quantifies how leakage behavior from afforesting agricultural land affects the intensification of agricultural production. In particular, we examine the leakage percentage from carbon offset allowance at specific southern regions in the United States as a part of a carbon market. We use the Forest and Agriculture Sector Optimization Model-Greenhouse Gases model to examine responses between sectors as part of the regional afforestation policy analysis. Regional characteristics and a policy's time frame are found to play important roles in achieving net gains, in terms of greenhouse gases stored, from such regional policies. In some cases, however, leakage greater than 100% is evident. C1 [Haim, David; White, Eric M.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Alig, Ralph J.] US Forest Serv, USDA, Pacific NW Res Stn, Washington, DC USA. RP Haim, D (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. EM david.haim@oregonstate.edu NR 25 TC 0 Z9 0 U1 2 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD MAR PY 2016 VL 38 IS 1 BP 132 EP 151 DI 10.1093/aepp/ppv010 PG 20 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DF9KB UT WOS:000371678700007 ER PT J AU Lin, H Zhou, HP Xu, LY Zhu, HP Huang, HH AF Lin, Huan Zhou, Hongping Xu, Linyun Zhu, Heping Huang, Huanhua TI Effect of surfactant concentration on the spreading properties of pesticide droplets on Eucalyptus leaves SO BIOSYSTEMS ENGINEERING LA English DT Article DE Surfactant; Droplet; Spread property; Eucalyptus; Wetted area; Deposition position ID WATER REPELLENCY; LEAF SURFACES; RETENTION; PLANTS; EVAPORATION; HUMECTANTS; HERBICIDES; IMPACTION; TRICHOMES; CUTICLES AB The area wetted by 500-mu m diameter droplets of pesticide and deionised water placed at different positions on Eucalyptus urophylla x E. grandis (E.0 x E.g) and Eucalyptus tereticornis (E.t) leaves was determined at an air temperature of 30 degrees C and a relative humidity of 60%. Dimethyl dichiorovinyl phosphate (DDVP), and efficient cypermethrin (EC) were diluted 1000 times in deionised water. Solutions the pesticides were prepared with surfactant concentrations (SC) of 0.1%, 0.25%, 0.5%, and 1%. For comparison SC solutions with water and deionised water were also used. Droplet deposition positions were located in the interveinal area, midrib, and secondary vein on both adaxial and abaxial surfaces. Without surfactant, all droplets remained nearly spherical and did not spread on the leaf surfaces. With surfactant, the deionised water and pesticide droplets had distinct spreading properties. For deionised water, both the wetted area and spread effectiveness peaked at 0.1% SC, while for pesticide droplets the wetted area peaked at 1% SC. However, the optimum SC was 0.25%. Compared with droplets on E.u x E.g leaves, droplets spread more on E.t leaves. The adaxial surface had better wettability than the abaxial surface. For pesticide droplets, the wetted area was a minimum on the midrib and the wetted area on the secondary vein was slightly larger than that on the interveinal area. (C) 2016 IAgrE. Published by Elsevier Ltd. All rights reserved. C1 [Lin, Huan; Zhou, Hongping; Xu, Linyun] Nanjing Forestry Univ, Coll Mech & Elect Engn, Nanjing 210037, Jiangsu, Peoples R China. [Zhu, Heping] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Applicat Technol Res Unit, Wooster, OH 44691 USA. [Huang, Huanhua] Guangdong Forestry Res Inst, Guangzhou 510520, Guangdong, Peoples R China. RP Zhou, HP (reprint author), Nanjing Forestry Univ, Coll Mech & Elect Engn, Nanjing 210037, Jiangsu, Peoples R China. EM HuanLinphd@163.com; hpzhou@njfu.com.cn; lyxu@njfu.com.cn; heping.zhu@ars.usda.gov; hhh87@126.com FU Chinese National "Twelfth Five-Year" Science and Technology [2014BAD08B04] FX This work was supported by the Chinese National "Twelfth Five-Year" Science and Technology Support Program (2014BAD08B04). The authors gratefully acknowledge Haotian Zhang for setting up the experimental systems, Shannan Yi for analysing the images, the Academy of Forestry of Guangdong Province for providing the Eucalyptus, and the Seed Center of Nanjing Forestry University for the greenhouse cultivation. The writing suggestions of Xuesong Jiang and Qing Chen are also highly appreciated. NR 30 TC 1 Z9 1 U1 7 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 EI 1537-5129 J9 BIOSYST ENG JI Biosyst. Eng. PD MAR PY 2016 VL 143 BP 42 EP 49 DI 10.1016/j.biosystemseng.2016.01.003 PG 8 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA DG1PM UT WOS:000371840000005 ER PT J AU Sexton, ZF Hughes, TJ Wise, KA AF Sexton, Zachary F. Hughes, Teresa J. Wise, Kiersten A. TI Analyzing isolate variability of Macrophomina phaseolina from a regional perspective SO CROP PROTECTION LA English DT Article DE Charcoal rot; Macrophomina phaseolina; Isolate; Variability ID SUDDEN-DEATH SYNDROME; CHARCOAL ROT; UNITED-STATES; 1ST REPORT; FUSARIUM-VIRGULIFORME; RESISTANCE; CULTIVARS; NORTHERN; PROGRESS; IMPACT AB Sexton, Z.S., Hughes, T.J., and Wise, K.A. 20XX. Analyzing isolate variability of Macrophomina phaseolina from a regional perspective. Crop Protection. XX:XXXX. Charcoal rot of soybean (Glycine max L.), caused by Macrophomina phaseolina (Tassi) Goid, has been reported more frequently in the north central United States in recent years. Growth of isolates collected from the northern and southern U.S. was compared in vitro under various incubation temperatures, and isolate pathogenicity was assessed on cultivars adapted to each region. Results indicate that northern isolates had increased growth at 15 degrees C compared to isolates from southern states, while southern isolates grew 23% more at 40 degrees C compared to northern isolates. Isolates from both regions were pathogenic on charcoal rot resistant and susceptible cultivars adapted for each region. This is the first research to suggest the importance of screening germplasm under regional environmental conditions and with local or regionally adapted isolates when evaluating cultivar resistance to charcoal rot. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Sexton, Zachary F.; Wise, Kiersten A.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Hughes, Teresa J.] USDA ARS, W Lafayette, IN 47909 USA. [Sexton, Zachary F.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Hughes, Teresa J.] Monsanto Co, 700 Chesterfield Pkwy W, Chesterfield, MO 63017 USA. RP Wise, KA (reprint author), Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. EM kawise@purdue.edu FU United Soybean Board; United States Department of Agriculture: Agricultural Research Service FX We gratefully acknowledge the United Soybean Board and the United States Department of Agriculture: Agricultural Research Service for funding this research. We thank Judy Santini for statistical consulting and Derrick McFall for help with maintenance of experiments. NR 29 TC 1 Z9 1 U1 3 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAR PY 2016 VL 81 BP 9 EP 13 DI 10.1016/j.cropro.2015.11.012 PG 5 WC Agronomy SC Agriculture GA DF9AS UT WOS:000371653200002 ER PT J AU Culbreath, AK Selph, AC Williams, BW Kemerait, RC Srinivasan, R Abney, MR Tillman, BL Holbrook, CC Branch, WD AF Culbreath, A. K. Selph, A. C. Williams, B. W. Kemerait, R. C., Jr. Srinivasan, R. Abney, M. R. Tillman, B. L. Holbrook, C. C. Branch, W. D. TI Effects of new field resistant cultivars and in-furrow applications of phorate insecticide on tomato spotted wilt of peanut SO CROP PROTECTION LA English DT Article DE Field resistance; Integrated disease management; Tospovirus; Thrips; Frankliniella fusca ID SEEDING RATE; ROW PATTERN; REGISTRATION; VIRUS; MANAGEMENT; EPIDEMIOLOGY; TIFGUARD; NEMATODE; DISEASE; LINES AB Field experiments were conducted at Tifton, Georgia from 2008 to 2014 to determine the effects of new peanut (Arachis hypogaea) cultivars and in-furrow applications of phorate insecticide on severity of tomato spotted wilt (TSW) caused by Tomato spotted wilt virus. Several cultivars, including Florida-07, Georgia-06G, Georgia-07W, Georgia Greener, and Tifguard, had final incidence of TSW that were less than that of Georgia Green. In-furrow applications of phorate insecticide reduced incidence of TSW in Georgia Green in three experiments in which that cultivar was included. In-furrow application of phorate insecticide reduced incidence of TSW in most cases where incidence in nontreated plots was 10% or higher. Cultivars Georganic, and Georgia-10T, had final incidence of TSW that was lower than that of Georgia-06G, or Florida-07 within nontreated plots across 2011-2012. Georgia-10T and Georgia-12Y had final incidence that was lower than that of Georgia-06G, Georgia-07W, Georgia-09B and Georgia Greener across insecticide treatments in 2013. In-furrow application of phorate increased yields across cultivars in 2008-2009, and increased yield of Georgia-09B in 2010. In most other cases, phorate had no significant effect on yield. With higher levels of field resistance in most of these cultivars, especially those such as Georgia-12Y, benefits from use of phorate insecticide for TSW suppression were small, and typically did not result in yield increase. Based on these results, several of these cultivars should allow greater flexibility for insecticide choice for thrips management without increasing the risk of losses to TSW. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Culbreath, A. K.; Selph, A. C.; Williams, B. W.; Kemerait, R. C., Jr.] Univ Georgia, Dept Plant Pathol, Coastal Plain Expt Stn, Tifton, GA 31793 USA. [Srinivasan, R.; Abney, M. R.] Univ Georgia, Dept Entomol, Coastal Plain Expt Stn, Tifton, GA 31793 USA. [Tillman, B. L.] Univ Florida, Dept Agron, North Florida Res & Educ Ctr, Marianna, FL 32446 USA. [Holbrook, C. C.] USDA ARS, Crop Genet & Breeding Res Unit, Coastal Plain Expt Stn, Tifton, GA 31793 USA. [Branch, W. D.] Univ Georgia, Dept Crop & Soil Sci, Coastal Plain Expt Stn, Tifton, GA 31793 USA. RP Culbreath, AK (reprint author), Univ Georgia, Dept Plant Pathol, Coastal Plain Expt Stn, Tifton, GA 31793 USA. EM spotwilt@uga.edu FU Georgia Agricultural Commodity Commission for Peanuts; National Peanut Board; AMVAC Chemical Corporation FX The essential efforts of Michael Heath, Ron Hooks, Jonathan Roberts, Sam Holbrook, and Matthew Wiggins are gratefully acknowledged. This research was supported in part through grants from the Georgia Agricultural Commodity Commission for Peanuts, the National Peanut Board, and AMVAC Chemical Corporation, and through the provision of seed by the Georgia Seed Development Commission. NR 33 TC 0 Z9 0 U1 4 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAR PY 2016 VL 81 BP 70 EP 75 DI 10.1016/j.cropro.2015.12.002 PG 6 WC Agronomy SC Agriculture GA DF9AS UT WOS:000371653200011 ER PT J AU Iverson, L Knight, KS Prasad, A Herms, DA Matthews, S Peters, M Smith, A Hartzler, DM Long, R Almendinger, J AF Iverson, Louis Knight, Kathleen S. Prasad, Anantha Herms, Daniel A. Matthews, Stephen Peters, Matthew Smith, Annemarie Hartzler, Diane M. Long, Robert Almendinger, John TI Potential Species Replacements for Black Ash (Fraxinus nigra) at the Confluence of Two Threats: Emerald Ash Borer and a Changing Climate SO ECOSYSTEMS LA English DT Article DE assisted range expansion; invasive insect spread model; climate change; emerald ash borer; multiple forest threats; restoration; species distribution models ID AGRILUS-PLANIPENNIS FAIRMAIRE; EASTERN UNITED-STATES; COLEOPTERA BUPRESTIDAE; ASSISTED MIGRATION; HABITAT MODELS; MINNESOTA; HISTORY; COLONIZATION; BIODIVERSITY; MANAGEMENT AB The emerald ash borer (Agrilus planipennis; EAB) is causing widespread mortality of ash (Fraxinus spp.) and climate change is altering habitats of tree species throughout large portions of North America. Black ash (F. nigra), a moist-soil species common in the Northwoods of Minnesota, Wisconsin, and Michigan, USA, is under a double threat of losing habitat from climate change and near annihilation from EAB. Because black ash often occurs in nearly pure stands, planting non-ash species is a management strategy already underway or being planned for thousands of acres. Tools are needed to assist managers in prioritizing sites for early treatment and to select potential species to replace black ash. This study explores the implications of threats to black ash ecosystems using analyses of field data and models to assess both the threats to, and potential replacement species for, black ash in Minnesota. For our analysis we (1) assessed the status of ashes and co-occurring species in forest inventory plots throughout Minnesota; (2) modeled the risk of EAB attack for multiple years in Minnesota; (3) modeled potential impacts of climate change on tree species with current or potential future habitat in Minnesota; (4) evaluated species co-occurring with black ash in plots in Ohio and Michigan, southeast of Minnesota; and (5) synthesized these results to provide a classification for candidate replacement species, both from within Minnesota and from points farther south. Though this process is demonstrated for black ash in Minnesota, the elements to be considered and modeled would be similar for any other location with a pest or pathogen threat for a species which simultaneously faces a changing climate. C1 [Iverson, Louis; Knight, Kathleen S.; Prasad, Anantha; Matthews, Stephen; Peters, Matthew] USDA, Forest Serv, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. [Herms, Daniel A.; Hartzler, Diane M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Matthews, Stephen] Ohio State Univ, Sch Nat Resources, 2021 Coffey Rd, Columbus, OH 43210 USA. [Smith, Annemarie] US Green Bldg Council Cent Ohio Chapter, Columbus, OH 43214 USA. [Long, Robert] USDA, Forest Serv, No Res Stn, Irvine, PA 16329 USA. [Almendinger, John] Minnesota Dept Nat Resources, Div Forestry, 483 Peterson Rd, Grand Rapids, MN 55744 USA. RP Iverson, L (reprint author), USDA, Forest Serv, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. EM liverson@fs.fed.us RI Matthews, Stephen/D-1050-2012 FU Northern Research Station of US Forest Service; Ohio State University; Minnesota Department of Natural Resources FX The work was sponsored by the authors' primary research institutions: the Northern Research Station of the US Forest Service, The Ohio State University, and the Minnesota Department of Natural Resources. The authors are indebted to many, many organizations and individuals for the data represented here-the people who sampled the thousands of FIA plots and the others who provided the analysis and maps of ash and other species across the region; those who provided data on EAB abundance, major roads and traffic density, campground size and usage, the wood products industry, and human population density; those who sampled all the mosquito-laden plots in Michigan and Ohio; and those carrying out the planting experiment on the Chippewa National Forest. We are truly indebted to these people, for without their field-based and mapping efforts none of these studies would have been possible. Thanks to Rich McCullough, US Forest Service, for assistance with FIA queries. Thanks also to Tony D'Amato, Stephen Handler, Herman Shugart, and anonymous reviewers for helpful comments on the manuscript. NR 61 TC 1 Z9 1 U1 31 U2 59 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD MAR PY 2016 VL 19 IS 2 BP 248 EP 270 DI 10.1007/s10021-015-9929-y PG 23 WC Ecology SC Environmental Sciences & Ecology GA DG1AM UT WOS:000371797400003 ER PT J AU Cagnone, G Tsai, TS Srirattana, K Rossello, F Powell, DR Rohrer, G Cree, L Trounce, IA St John, JC AF Cagnone, Gael Tsai, Te-Sha Srirattana, Kanokwan Rossello, Fernando Powell, David R. Rohrer, Gary Cree, Lynsey Trounce, Ian A. St John, Justin C. TI Segregation of Naturally Occurring Mitochondrial DNA Variants in a Mini-Pig Model SO GENETICS LA English DT Article DE mitochondrial DNA; segregation; variants; generations; embryo ID RIBOSOMAL-RNA GENE; HETEROPLASMIC MICE; MTDNA MUTATIONS; COPY NUMBER; IN-VITRO; ADAPTIVE SELECTION; OOCYTE MATURATION; NUCLEAR TRANSFER; SKELETAL-MUSCLE; SEX-DIFFERENCES AB The maternally inherited mitochondrial genome (mtDNA) is present in multimeric form within cells and harbors sequence variants (heteroplasmy). While a single mtDNA variant at high load can cause disease, naturally occurring variants likely persist at low levels across generations of healthy populations. To determine how naturally occurring variants are segregated and transmitted, we generated a mini-pig model, which originates from the same maternal ancestor. Following next-generation sequencing, we identified a series of low-level mtDNA variants in blood samples from the female founder and her daughters. Four variants, ranging from 3% to 20%, were selected for validation by high-resolution melting analysis in 12 tissues from 31 animals across three generations. All four variants were maintained in the offspring, but variant load fluctuated significantly across the generations in several tissues, with sex-specific differences in heart and liver. Moreover, variant load was persistently reduced in high-respiratory organs (heart, brain, diaphragm, and muscle), which correlated significantly with higher mtDNA copy number. However, oocytes showed increased heterogeneity in variant load, which correlated with increased mtDNA copy number during in vitro maturation. Altogether, these outcomes show that naturally occurring mtDNA variants segregate and are maintained in a tissue-specific manner across generations. This segregation likely involves the maintenance of selective mtDNA variants during organogenesis, which can be differentially regulated in oocytes and preimplantation embryos during maturation. C1 [Cagnone, Gael; Tsai, Te-Sha; Srirattana, Kanokwan; St John, Justin C.] Hudson Inst Med Res, Ctr Genet Dis, 31 Wright St, Clayton, Vic 3168, Australia. [Cagnone, Gael; Tsai, Te-Sha; Srirattana, Kanokwan; St John, Justin C.] Monash Univ, Dept Mol & Translat Sci, Clayton, Vic 3168, Australia. [Rossello, Fernando; Powell, David R.] Monash Univ, Victorian Bioinformat Consortium, Clayton, Vic 3168, Australia. [Rossello, Fernando; Powell, David R.] Victorian Life Sci Computat Initiat, Life Sci Computat Ctr, Parkville, Vic 3052, Australia. [Rohrer, Gary] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Cree, Lynsey] Univ Auckland, Fac Med & Hlth Sci, Dept Obstet & Gynaecol, Auckland 1023, New Zealand. [Trounce, Ian A.] Univ Melbourne, Royal Victorian Eye & Ear Hosp, Dept Surg, Ctr Eye Res Australia,Ophthalmol, East Melbourne, Vic 3002, Australia. RP St John, JC (reprint author), Hudson Inst Med Res, Ctr Genet Dis, 31 Wright St, Clayton, Vic 3168, Australia. EM justin.stjohn@hudson.org.au OI Cree, Lynsey/0000-0003-2090-1589; Trounce, Ian/0000-0002-1070-8892 FU National Health and Medical Research Council [GNT1041471]; Victorian Government's Operational Infrastructure Support Program; Monash Medical Centre Animal Ethics Committee A (MMCA) [MMCA/2012/84] FX This work was supported by the National Health and Medical Research Council (GNT1041471 to J.C.S. and I.A.T.) and the Victorian Government's Operational Infrastructure Support Program. Approval for the use of animals was granted by Monash Medical Centre Animal Ethics Committee A (MMCA), approval no.: MMCA/2012/84. NR 84 TC 2 Z9 2 U1 0 U2 2 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD MAR PY 2016 VL 202 IS 3 BP 931 EP + DI 10.1534/genetics.115.181321 PG 28 WC Genetics & Heredity SC Genetics & Heredity GA DF8GT UT WOS:000371596400010 PM 26819245 ER PT J AU Ambrose, A Kandpal, LM Kim, MS Lee, WH Cho, BK AF Ambrose, Ashabahebwa Kandpal, Lalit Mohan Kim, Moon S. Lee, Wang-Hee Cho, Byoung-Kwan TI High speed measurement of corn seed viability using hyperspectral imaging SO INFRARED PHYSICS & TECHNOLOGY LA English DT Article DE Hyperspectral imaging; Corn seed; Viability; PLS-DA; Image processing ID NEAR-INFRARED SPECTROSCOPY; KERNELS; IDENTIFICATION; SPECTRA; STORAGE; SYSTEM; DAMAGE AB Corn is one of the most cultivated crops all over world as food for humans as well as animals. Optimized agronomic practices and improved technological interventions during planting, harvesting and post-harvest handling are critical to improving the quantity and quality of corn production. Seed germination and vigor are the primary determinants of high yield notwithstanding any other factors that may play during the growth period. Seed viability may be lost during storage due to unfavorable conditions e.g. moisture content and temperatures, or physical damage during mechanical processing e.g. shelling, or over heating during drying. It is therefore vital for seed companies and farmers to test and ascertain seed viability to avoid losses of any kind. This study aimed at investigating the possibility of using hyperspectral imaging (HSI) technique to discriminate viable and nonviable corn seeds. A group of corn samples were heat treated by using microwave process while a group of seeds were kept as control group (untreated). The hyperspectral images of corn seeds of both groups were captured between 400 and 2500 nm wave range. Partial least squares discriminant analysis (PLS-DA) was built for the classification of aged (heat treated) and normal (untreated) corn seeds. The model showed highest classification accuracy of 97.6% (calibration) and 95.6% (prediction) in the SWIR region of the HSI. Furthermore, the PLS-DA and binary images were capable to provide the visual information of treated and untreated corn seeds. The overall results suggest that HSI technique is accurate for classification of viable and non-viable seeds with non-destructive manner. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ambrose, Ashabahebwa; Kandpal, Lalit Mohan; Lee, Wang-Hee; Cho, Byoung-Kwan] Chungnam Natl Univ, Coll Agr & Life Sci, Dept Biosyst Machinery Engn, 99 Daehak Ro, Taejon 305764, South Korea. [Kim, Moon S.] ARS, Environm Microbiol & Food Safety Lab, USDA, BARC East, Powder Mill Rd Bldg 303, Beltsville, MD 20705 USA. RP Cho, BK (reprint author), Chungnam Natl Univ, Coll Agr & Life Sci, Dept Biosyst Machinery Engn, 99 Daehak Ro, Taejon 305764, South Korea. EM ashabahebwa.ambrose@gmail.com; lalitm85@gmail.com; moon.kim@ars.usda.gov; wanghee@cnu.ac.kr; chobk@cnu.ac.kr FU Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA); Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA); Ministry of Oceans and Fisheries (MOF); Rural Development Administration (RDA); Korea Forest Service (KFS). Republic of Korea FX This research was partially supported by Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA) and by Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS). Republic of Korea. NR 19 TC 2 Z9 2 U1 22 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1350-4495 EI 1879-0275 J9 INFRARED PHYS TECHN JI Infrared Phys. Technol. PD MAR PY 2016 VL 75 BP 173 EP 179 DI 10.1016/j.infrared.2015.12.008 PG 7 WC Instruments & Instrumentation; Optics; Physics, Applied SC Instruments & Instrumentation; Optics; Physics GA DF7RR UT WOS:000371555800026 ER PT J AU Meadows, A Lee, JH Wu, CS Wei, Q Pradhan, G Yafi, M Lu, HC Sun, Y AF Meadows, A. Lee, J. H. Wu, C-S Wei, Q. Pradhan, G. Yafi, M. Lu, H-C Sun, Y. TI Deletion of G-protein-coupled receptor 55 promotes obesity by reducing physical activity SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article ID DIET-INDUCED OBESITY; ADIPOSE-TISSUE; CANNABINOID RECEPTOR; POTENTIAL ROLE; CB1 RECEPTOR; ENDOCANNABINOID SYSTEM; PARKINSONS-DISEASE; ENERGY HOMEOSTASIS; VOLUNTARY EXERCISE; FOOD-INTAKE AB BACKGROUND/OBJECTIVES: Cannabinoid receptor 1 (CB1) is the best-characterized cannabinoid receptor, and CB1 antagonists are used in clinical trials to treat obesity. Because of the wide range of CB1 functions, the side effects of CB1 antagonists pose serious concerns. G-protein-coupled receptor 55 (GPR55) is an atypical cannabinoid receptor, and its pharmacology and functions are distinct from CB1. GPR55 regulates neuropathic pain, gut, bone, immune functions and motor coordination. GPR55 is expressed in various brain regions and peripheral tissues. However, the roles of GPR55 in energy and glucose homeostasis are unknown. Here we have investigated the roles of GPR55 in energy balance and insulin sensitivity using GPR55-null mice (GPR55(-/-)). METHODS: Body composition of the mice was measured by EchoMRI. Food intake, feeding behavior, energy expenditure and physical activity of GPR55(-/-) mice were determined by indirect calorimetry. Muscle function was assessed by forced treadmill running test. Insulin sensitivity was evaluated by glucose and insulin tolerance tests. Adipose inflammation was assessed by flow cytometry analysis of adipose tissue macrophages. The expression of inflammatory markers in adipose tissues and orexigenic/anorexigenic peptides in the hypothalamus was also analyzed by real-time PCR. RESULTS: GPR55(-/-) mice had normal total energy intake and feeding pattern (i.e., no changes in meal size, meal number or feeding frequency). Intriguingly, whereas adult GPR55(-/-) mice only showed a modest increase in overall body weight, they exhibited significantly increased fat mass and insulin resistance. The spontaneous locomotor activity of GPR55(-/-) mice was dramatically decreased, whereas resting metabolic rate and non-shivering thermogenesis were unchanged. Moreover, GPR55(-/-) mice exhibited significantly decreased voluntary physical activity, showing reduced running distance on the running wheels, whereas muscle function appeared to be normal. CONCLUSIONS: GPR55 has an important role in energy homeostasis. GPR55 ablation increases adiposity and insulin resistance by selectively decreasing physical activity, but not by altering feeding behavior as CB1. C1 [Meadows, A.; Lee, J. H.; Wu, C-S; Wei, Q.; Pradhan, G.; Sun, Y.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, One Baylor Plaza,MS320,1100 Bates Ave Room 5024, Houston, TX 77030 USA. [Meadows, A.; Yafi, M.] Univ Texas Med Sch Houston, Dept Pediat, Div Pediat Endocrinol, Houston, TX USA. [Wei, Q.] Southeast Univ, Zhongda Hosp, Div Endocrinol, Nanjing, Jiangsu, Peoples R China. [Lu, H-C] Indiana Univ, Dept Psychol & Brain Sci, Bloomington, IN USA. [Sun, Y.] Baylor Coll Med, Huffington Ctr Aging, Dept Mol & Cellular Biol, Houston, TX 77030 USA. RP Sun, Y (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, One Baylor Plaza,MS320,1100 Bates Ave Room 5024, Houston, TX 77030 USA. EM yuxiangs@bcm.edu FU USDA/CRIS [ARS 3092-5-001-059]; American Diabetes Association [1-15-BS-177]; American Heart Association [12IRG9230004, 14GRNT18990019]; [P30 DK56338] FX AM, JHL and CSW conducted the experiments, analyzed the data and wrote the manuscript. QW and GP conducted the experiments. MY supported the study and proofread the paper. HCL provided GPR55 mice, consulted on the study and proofread the paper. YS designed the experiments and wrote the manuscript. All authors read and approved the final manuscript. Indirect calorimetry was performed in the Mouse Metabolic Research Unit at the Children's Nutrition Research Center, Baylor College of Medicine. We acknowledge the expert assistance of Mr Firoz Vohra and the MMRU Core Director Dr Marta Fiorotto. We thank Mr Michael R Honig at Houston's Community Public Radio Station KPFT for his editorial assistance. This study was supported by USDA/CRIS grant ARS 3092-5-001-059 (YS), American Diabetes Association #1-15-BS-177 (YS), American Heart Association grants 12IRG9230004 and 14GRNT18990019 (YS), and was also partially supported by P30 DK56338 (YS). NR 60 TC 6 Z9 6 U1 1 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 EI 1476-5497 J9 INT J OBESITY JI Int. J. Obes. PD MAR PY 2016 VL 40 IS 3 BP 417 EP 424 DI 10.1038/ijo.2015.209 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA DG1BF UT WOS:000371799300005 PM 26447738 ER PT J AU Zhou, XJ Zhang, LD Shi, CL Fratamico, PM Liu, B Paoli, GC Dan, XL Zhuang, XF Cui, Y Wang, DP Shi, XM AF Zhou, Xiujuan Zhang, Lida Shi, Chunlei Fratamico, Pina M. Liu, Bin Paoli, George C. Dan, Xianlong Zhuang, Xiaofei Cui, Yan Wang, Dapeng Shi, Xianming TI Genome-Scale Screening and Validation of Targets for Identification of Salmonella enterica and Serovar Prediction SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Comparative genomics; Detection targets; Salmonella enterica; Serovar prediction ID POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; MULTIPLEX PCR; TYPHIMURIUM; SEQUENCE; PATHOGENS; ASSAY; FOOD; ENTERITIDIS; SEROTYPES AB Salmonella enterica is the most common foodbome pathogen worldwide, with 2,500 recognized serovars. Detection of S. enterica and its classification into serovars are essential for food safety surveillance and clinical diagnosis. The PCR method is useful for these applications because of its rapidity and high accuracy. We obtained 412 candidate detection targets for S. enterica using a comparative genomics mining approach. Gene ontology (GO) functional enrichment analysis of these candidate targets revealed that the GO term with the largest number of unigenes with known function (38 of 177, 21.5%) was significantly involved in pathogenesis (P < 10(-24)). All the candidate targets were then evaluated by PCR assays. Fifteen targets showed high specificity for the detection of S. enterica by verification with 151 S. enterica strains and 34 non-Salmonella strains. The phylogenetic trees of verified targets were highly comparable with those of housekeeping genes, especially for differentiating S. enterica strains into serovars. The serovar prediction ability was validated by sequencing one target (S9) for 39 S. enterica strains belonging to six serovars. Identical mutation sites existed in the same serovar, and different mutation sites were found in diverse serovars. Our findings revealed that 15 verified targets can be potentially used for molecular detection, and some of them can be used for serotyping of S. enterica strains. C1 [Zhou, Xiujuan; Zhang, Lida; Shi, Chunlei; Dan, Xianlong; Zhuang, Xiaofei; Cui, Yan; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, Chinese Minist Sci & Technol, Shanghai 200240, Peoples R China. [Zhou, Xiujuan; Zhang, Lida; Shi, Chunlei; Dan, Xianlong; Zhuang, Xiaofei; Cui, Yan; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, USDA, Joint Res Ctr Food Safety, Sch Agr & Biol, Shanghai 200240, Peoples R China. [Zhou, Xiujuan; Zhang, Lida; Shi, Chunlei; Dan, Xianlong; Zhuang, Xiaofei; Cui, Yan; Wang, Dapeng; Shi, Xianming] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China. [Fratamico, Pina M.; Paoli, George C.] USDA, Agr Res Serv, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Liu, Bin] Northwest Agr & Forestry Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. RP Shi, XM (reprint author), Shanghai Jiao Tong Univ, Chinese Minist Sci & Technol, Shanghai 200240, Peoples R China.; Shi, XM (reprint author), Shanghai Jiao Tong Univ, USDA, Joint Res Ctr Food Safety, Sch Agr & Biol, Shanghai 200240, Peoples R China.; Shi, XM (reprint author), Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China. EM xmshi@sjtu.edu.cn FU National Natural Science Foundation of China [31230058]; Ministry of Science and Technology of China [2012AA101601, 2012BAD29B02]; Science and Technology Commission of Shanghai Municipality [14390711900]; General Administration of Quality Supervision, Inspection, and Quarantine of China [2014IK123] FX This research was supported by the National Natural Science Foundation of China (31230058), the Ministry of Science and Technology of China (2012AA101601 and 2012BAD29B02), the Science and Technology Commission of Shanghai Municipality (14390711900), and the General Administration of Quality Supervision, Inspection, and Quarantine of China (2014IK123). NR 35 TC 0 Z9 0 U1 3 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2016 VL 79 IS 3 BP 376 EP 383 DI 10.4315/0362-028X.JFP-15-286 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DF7SF UT WOS:000371557200004 PM 26939647 ER PT J AU Stromberg, ZR Lewis, GL Aly, SS Lehenbauer, TW Bosilevac, JM Cernicchiaro, N Moxley, RA AF Stromberg, Zachary R. Lewis, Gentry L. Aly, Sharif S. Lehenbauer, Terry W. Bosilevac, Joseph M. Cernicchiaro, Natalia Moxley, Rodney A. TI Prevalence and Level of Enterohemorrhagic Escherichia coli in Culled Dairy Cows at Harvest SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Dairy cows; Enterohemorrhagic Escherichia coli; Escherichia coli; Shiga toxin producing Escherichia coli ID BEEF PROCESSING PLANTS; TIME PCR ASSAYS; MULTIPLEX PCR; CATTLE FECES; FEEDLOT CATTLE; UNITED-STATES; WASHINGTON-STATE; NON-O157; O157; HIDES AB The primary objective of this study was to determine the prevalence and level of enterohemorrhagic Escherichia coli (EHEC) 026, 045, 0103, 0111, 0121, and 0145 (collectively EHEC-6) plus EHEC 0157 in fecal, hide, and preintervention carcass surface samples from culled dairy cows. Matched samples (n = 300) were collected from 100 cows at harvest and tested by a culture-based method and two molecular methods: NeoSEEK STEC (NS) and Atlas STEC EG2 Combo. Both the culture and NS methods can be used to discriminate among the seven EHEC types (EHEC-7), from which the cumulative prevalence was inferred, whereas the Atlas method can discriminate only between EHEC O157 and non-O157 EHEC, without discrimination of the serogroup. The EHEC-7 prevalence in feces, hides, and carcass surfaces was 6.5, 15.6, and 1.0%, respectively, with the culture method and 25.9, 64.9, and 7.0%, respectively, with the NS method. With the Atlas method, the prevalence of non-O157 EHEC was 29.1, 38.3, and 28.0% and that of EHEC O157 was 29.1, 57.0, and 3.0% for feces, hides, and carcasses, respectively. Only two samples (a hide sample and a fecal sample) originating from different cows contained quantifiable EHEC. In both samples, the isolates were identified as EHEC O157, with 4.7 CFU/1,000 cm(2) in the hide sample and 3.9 log CFU/g in the fecal sample. Moderate agreement was found between culture and NS results for detection of EHEC 026 (kappa = 0.58, P < 0.001), EHEC 0121 (kappa = 0.50, P < 0.001), and EHEC O157 (kappa = 0.40, P < 0.001). No significant agreement was observed between NS and Atlas results or between culture and Atlas results. Detection of an EHEC serogroup in fecal samples was significantly associated with detection of the same EHEC serogroup in hide samples for EHEC 026 (P = 0.001), EHEC 0111 (P = 0.002), EHEC 0121 (P < 0.001), and EHEC-6 (P = 0.029) based on NS detection and for EHEC 0121 (P < 0.001) based on detection by culture. This study provides evidence that non-O157 EHEC are ubiquitous on hides of culled dairy cattle and that feces are an important source of non-O157 EHEC hide contamination. C1 [Stromberg, Zachary R.; Lewis, Gentry L.; Moxley, Rodney A.] Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE 68583 USA. [Aly, Sharif S.; Lehenbauer, Terry W.] Univ Calif Davis, Vet Med Teaching & Res Ctr, Sch Vet Med, Tulare, CA 93274 USA. [Aly, Sharif S.; Lehenbauer, Terry W.] Univ Calif Davis, Dept Populat Hlth & Reprod, Sch Vet Med, Davis, CA 95616 USA. [Bosilevac, Joseph M.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. [Cernicchiaro, Natalia] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. RP Moxley, RA (reprint author), Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE 68583 USA. EM rmoxley1@unl.edu OI Moxley, Rodney/0000-0002-5377-7716 FU National Institute of Food and Agriculture [2012-68003-30155]; U.S. Department of Agriculture (USDA) FX The authors thank Isha Patel for conducting the ECID tests, the owners and management of the participating processing plant, and Paul Rossitto, Dr. Deniece Williams, Jennifer Crook, Arianna Villarreal, Hannah Frolli, Greg Smith, and Lawnie Luedtke for technical assistance. This material is based upon work that is supported by the U.S. Department of Agriculture (USDA), National Institute of Food and Agriculture under award 2012-68003-30155. No competing financial interests exist. Trade names are necessary to accurately report experimental results. The use of trade names by a USDA author implies no approval of the product to the exclusion of others that may also be suitable. The USDA neither guarantees nor warrants the standard of any products mentioned. NR 74 TC 3 Z9 3 U1 0 U2 10 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2016 VL 79 IS 3 BP 421 EP 431 DI 10.4315/0362-028X.JFP-15-368 PG 11 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DF7SF UT WOS:000371557200009 PM 26939652 ER PT J AU Robertson, K Green, A Allen, L Ihry, T White, P Chen, WS Douris, A Levine, J AF Robertson, Kis Green, Alice Allen, Latasha Ihry, Timothy White, Patricia Chen, Wu-San Douris, Aphrodite Levine, Jeoffrey TI Foodborne Outbreaks Reported to the US Food Safety and Inspection Service, Fiscal Years 2007 through 2012 SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Foodborne outbreaks; Illness; Meat; Poultry ID ACTIVE SURVEILLANCE NETWORK; UNITED-STATES; DISEASE OUTBREAKS; PATHOGENS; ILLNESS; SITES; COMMODITIES; INFECTION AB The U.S. Department of Agriculture's Food Safety and Inspection Service (FSIS) works closely with federal, state, and local public health partners to investigate foodborne illness outbreaks associated with its regulated products. To provide insight into outbreaks associated with meat and poultry, outbreaks reported to FSIS during fiscal years 2007 through 2012 were evaluated. Outbreaks were classified according to the strength of evidence linking them to an FSIS-regulated product and by their epidemiological, etiological, and vehicle characteristics. Differences in outbreak characteristics between the period 2007 through 2009 and the period 2010 through 2012 were assessed using a chi-square test or Mann-Whitney U test. Of the 163 reported outbreaks eligible for analysis, 89 (55%) were identified as possibly linked to FSIS-regulated products and 74 (45%) were definitively linked to FSIS-regulated products. Overall, these outbreaks were associated with 4,132 illnesses, 772 hospitalizations, and 19 deaths. Shiga toxin producing Escherichia coli was associated with the greatest proportion of reported outbreaks (55%), followed by Salmonella enterica (34%) and Listeria monocytogenes (7%). Meat and poultry products commercially sold as raw were linked to 125 (77%) outbreaks, and of these, 105 (80%) involved beef. Over the study period, the number of reported outbreaks definitively linked to FSIS-regulated products (P = 0.03) declined, while the proportion of culture-confirmed cases (P = 0.0001) increased. Our findings provide insight into the characteristics of outbreaks associated with meat and poultry products. C1 [Robertson, Kis; Green, Alice; Allen, Latasha; Ihry, Timothy; White, Patricia; Chen, Wu-San; Douris, Aphrodite; Levine, Jeoffrey] USDA, Food Safety & Inspect Serv, Off Publ Hlth Sci, Washington, DC 20250 USA. RP Robertson, K (reprint author), USDA, Food Safety & Inspect Serv, Off Publ Hlth Sci, Washington, DC 20250 USA. EM kis.robertson@fsis.usda.gov NR 21 TC 3 Z9 3 U1 4 U2 17 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2016 VL 79 IS 3 BP 442 EP 447 DI 10.4315/0362-028X.JFP-15-376 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DF7SF UT WOS:000371557200011 PM 26939654 ER PT J AU Palumbo, JD O'Keeffe, TL Ho, YS Fidelibus, MW AF Palumbo, Jeffrey D. O'Keeffe, Teresa L. Ho, Yvonne S. Fidelibus, Matthew W. TI Population Dynamics of Aspergillus Section Nigri Species on Vineyard Samples of Grapes and Raisins SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Aspergillus carbonarius; Aspergillus niger; Droplet digital PCR; Fungal ecology; Ochratoxin ID OCHRATOXIN-A OCCURRENCE; PORTUGUESE WINE GRAPES; BLACK ASPERGILLI; WATER ACTIVITY; CARBONARIUS; FRUITS; FUNGI; CONTAMINATION; DIVERSITY; IDENTIFICATION AB Several species of Aspergillus section Nigri, including potential mycotoxin producers, are common residents of grape vineyards, but the relative population size of individual species throughout the growing season is difficult to determine using traditional isolation and identification methods. Using a quantitative droplet digital PCR (ddPCR) method in combination with dilution plating, total Aspergillus section Nigri populations and relative proportions of A. niger, A. welwitschiae, A. carbonarius, and A. tubingensis were measured from vineyard samples without the need for identifying individual fungal isolates. Grapes were sampled from two raisin vineyards (vineyards A and B) at berry set, veraison, harvest, and raisin stages in two consecutive years. Plate counts showed that the total population of Aspergillus section Nigri present on the fruit increased from berry set to raisin and became a larger component of the total recovered fungal population in both vineyards in both years. Results from ddPCR analysis showed that the relative proportion of A. carbonarius among the four species assayed increased later in the season (harvest and raisin) in comparison to earlier in the season (berry set and veraison). Total fungal and Aspergillus section Nigri plate counts were not significantly different between vineyards in either year. However, vineyard A generally showed higher proportions of A. carbonarius in harvest and raisin samples than vineyard B. This coincided with higher incidence and levels of ochratoxin A in vineyard A harvest and raisin fruit than in vineyard B fruit. This work demonstrates that this ddPCR method is a useful tool for culture-independent monitoring of populations of mycotoxigenic Aspergillus species during grape and raisin production. C1 [Palumbo, Jeffrey D.; O'Keeffe, Teresa L.; Ho, Yvonne S.] USDA, ARS, Foodborne Toxin Detect & Prevent Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Fidelibus, Matthew W.] Univ Calif Parlier, Kearney Agr Res & Extens Ctr, 9240 South Riverbend Ave, Parlier, CA 93648 USA. RP Palumbo, JD (reprint author), USDA, ARS, Foodborne Toxin Detect & Prevent Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM jeffrey.palumbo@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service CRIS project [2030-42000-038-00D] FX We thank S. Cisneros, J. Osorio-Aguilar, R. Fox, J. Govoni, and M. Ok for sample collection and processing. This work was supported by U.S. Department of Agriculture, Agricultural Research Service CRIS project 2030-42000-038-00D. NR 36 TC 0 Z9 0 U1 5 U2 12 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAR PY 2016 VL 79 IS 3 BP 448 EP 453 DI 10.4315/0362-028X.JFP-15-437 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DF7SF UT WOS:000371557200012 PM 26939655 ER PT J AU Zhang, M Liu, Z Jan, CC AF Zhang, Ming Liu, Zhao Jan, Chao-Chien TI Molecular mapping of a rust resistance gene R-14 in cultivated sunflower line PH 3 SO MOLECULAR BREEDING LA English DT Article DE Sunflower; Rust resistance; Simple sequence repeat (SSR); Single nucleotide polymorphism (SNP); Marker-assisted breeding ID FERTILITY RESTORATION GENE; HELIANTHUS-ANNUUS L.; CONFECTION SUNFLOWER; PUCCINIA-HELIANTHI; DISEASE-RESISTANCE; IDENTIFICATION; GERMPLASMS; MARKERS; GENOME; LOCI AB Sunflower, the fifth largest oilseed crop in the world, plays an important role in human diets. Recently, sunflower production in North America has suffered serious yield losses from newly evolved races of sunflower rust (Puccinia helianthi Schwein.). The rust resistance gene, designated R-14, in a germplasm line PH 3 originated from a wild Helianthus annuus L. population resistant to 11 rust races. PH 3 has seedling with an extraordinary purple hypocotyl color. The objectives of this study were to map both the R-14 rust resistance gene and the purple hypocotyl gene-designated PHC in PH 3, and to identify molecular markers for marker-assisted breeding for sunflower rust resistance. A set of 517 mapped SSR/InDel and four SNP markers was used to detect polymorphisms between the parents. Fourteen markers covering a genetic distance of 17.0 cM on linkage group (LG) 11 were linked to R-14. R-14 was mapped to the middle of the LG, with a dominant SNP marker NSA_000064 as the closest marker at a distance of 0.7 cM, and another codominant marker ORS542 linked at 3.5 cM proximally. One dominant marker ZVG53 was linked on the distal side at 6.9 cM. The PHC gene was also linked to R-14 with a distance of 6.2 cM. Chi-squared analysis of the segregation ratios of R-14, PHC, and ten linked markers indicated a deviation from an expected 1:2:1 or 3:1 ratio. The closely linked molecular or morphological markers could facilitate sunflower rust-resistant breeding and accelerate the development of rust-resistant hybrids. C1 [Zhang, Ming] Heilongjiang Acad Agr Sci, Inst Ind Crops, Harbin 150001, Peoples R China. [Zhang, Ming] Northeast Agr Univ, Harbin 150030, Peoples R China. [Liu, Zhao] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Jan, Chao-Chien] USDA ARS, No Crop Sci Lab, 1307 18th St N, Fargo, ND 58102 USA. RP Jan, CC (reprint author), USDA ARS, No Crop Sci Lab, 1307 18th St N, Fargo, ND 58102 USA. EM hljsunflower@hotmail.com; zhao.liu@ndsu.edu; chaochien.jan@ars.usda.gov FU Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin, China FX The authors thank Lisa Brown, Angelia Hogness, Wei Yue, and Dr. Guojia Ma for technical assistance, and Drs. Lili Qi, Li Gong, Hongxia Wang, and Zahirul Talukder for important discussion. Dr. Lili Qi kindly provided the spores for race 336 for inoculation and modified SNP protocol and primers. We would also like to thank Drs. Gerald J. Seiler, Larry G. Campbell, Lili Qi, and Steven S. Xu for their critical review of this manuscript. Part of this work was supported by a grant to M. Zhang from the Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin, China. NR 57 TC 0 Z9 0 U1 3 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD MAR PY 2016 VL 36 IS 3 AR 32 DI 10.1007/s11032-016-0456-0 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DF6IN UT WOS:000371459200012 ER PT J AU Shah, RV Murthy, VL Allison, MA Ding, J Budoff, M Frazier-Wood, AC Lima, JAC Steffen, L Siscovick, D Tucker, KL Ouyang, P Abbasi, SA Danielson, K Jerosch-Herold, M Mozaffarian, D AF Shah, R. V. Murthy, V. L. Allison, M. A. Ding, J. Budoff, M. Frazier-Wood, A. C. Lima, J. A. C. Steffen, L. Siscovick, D. Tucker, K. L. Ouyang, P. Abbasi, S. A. Danielson, K. Jerosch-Herold, M. Mozaffarian, D. TI Diet and adipose tissue distributions: The Multi-Ethnic Study of Atherosclerosis SO NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES LA English DT Article DE Adiposity; Obesity; Diet; Inflammation; Body mass index ID SUGAR-SWEETENED BEVERAGE; FATTY LIVER-DISEASE; MEDITERRANEAN DIET; CARDIOVASCULAR-DISEASE; VISCERAL ADIPOSITY; ABDOMINAL FAT; HEART-DISEASE; OBESE ADULTS; LIFE-STYLE; RISK AB Background and aims: Dietary quality affects cardiometabolic risk, yet its pathways of influence on regional adipose tissue depots involved in metabolic and diabetes risk are not well established. We aimed to investigate the relationship between dietary quality and regional adiposity. Methods and results: We investigated 5079 individuals in the Multi-Ethnic Study of Atherosclerosis (MESA) who had food-frequency questionnaires and measurement of pericardial fat and hepatic attenuation at the baseline study visit in MESA, as well as a subgroup with imaging for visceral and subcutaneous fat (N = 1390). A dietary quality score (DietQuality) was constructed to include established food group constituents of a Mediterranean-type diet. Linear models estimated associations of dietary score as well as its constituents with regional adiposity. Baseline mean age was 61 (+/- 10) years, and approximately half of the participants (47%) were male. Those with a higher DietQuality score were generally older, female, with a lower body mass index, C-reactive protein, and markers of insulin resistance. After adjustment, a higher DietQuality score was associated with lower visceral fat (lowest vs. highest dietary score quartile: 523.6 vs. 460.5 cm(2)/m; P < 0.01 for trend), pericardial fat (47.5 vs. 41.3 cm(3)/m; P < 0.01 for trend), lesser hepatic steatosis (by hepatic attenuation; 58.6 vs. 60.7 Hounsfield units; P < 0.01 for trend), but not subcutaneous fat (P = 0.39). Greater fruits, vegetables, whole grains, seeds/nuts and yogurt intake were associated with decreased adiposity, while red/processed meats were associated with greater regional adiposity. Conclusion: A higher quality diet pattern is associated with less regional adiposity, suggesting a potential mechanism of beneficial dietary effects on diabetes, metabolic, and cardiovascular risk. (C) 2016 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved. C1 [Shah, R. V.; Danielson, K.] Beth Israel Deaconess Med Ctr, Dept Med, Cardiovasc Div, 330 Brookline Ave, Boston, MA 02215 USA. [Murthy, V. L.] Univ Michigan, Dept Med, Cardiovasc Med Div, Ann Arbor, MI 48109 USA. [Murthy, V. L.] Univ Michigan, Dept Radiol, Div Nucl Med, Ann Arbor, MI 48109 USA. [Allison, M. A.] Univ Calif San Diego, Dept Family & Prevent Med, La Jolla, CA 92093 USA. [Ding, J.] Wake Forest Univ, Bowman Gray Sch Med, Winston Salem, NC USA. [Budoff, M.] Univ Calif Los Angeles, Dept Cardiol & Med, Los Angeles, CA USA. [Frazier-Wood, A. C.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lima, J. A. C.; Ouyang, P.] Johns Hopkins Univ, Dept Med, Div Cardiol, Baltimore, MD USA. [Steffen, L.] Univ Minnesota, Sch Publ Hlth, Minneapolis, MN USA. [Siscovick, D.] Univ Washington, Sch Publ Hlth, Seattle, WA 98195 USA. [Tucker, K. L.] Univ Massachusetts, Lowell Sch Publ Hlth, Lowell, MA USA. [Abbasi, S. A.] Brown Univ, Dept Cardiol, Providence, RI 02912 USA. [Jerosch-Herold, M.] Brigham & Womens Hosp, Dept Med, Cardiovasc Div, Noninvas Cardiovasc Imaging Sect, 75 Francis St, Boston, MA 02115 USA. [Jerosch-Herold, M.] Brigham & Womens Hosp, Dept Radiol, 75 Francis St, Boston, MA 02115 USA. [Mozaffarian, D.] Tufts Univ, Sch Publ Hlth, Boston, MA 02111 USA. RP Murthy, VL (reprint author), Univ Michigan, 1500 E Med Ctr Dr,SPC 5873, Ann Arbor, MI 48109 USA.; Shah, RV (reprint author), Beth Israel Deaconess Med Ctr, 330 Brookline Ave, Boston, MA 02215 USA.; Mozaffarian, D (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Boston, MA 02111 USA. EM rshah1@bidmc.harvard.edu; vlmurthy@med.umich.edu; darius.mozaffarian@tufts.edu OI Murthy, Venkatesh/0000-0002-7901-1321; Allison, Matthew/0000-0003-0777-8272 FU National Heart, Lung, and Blood Institute [N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, N01-HC-95169]; National Center for Research Resources [UL1-TR-000040, UL1-TR-001079]; American Heart Association; [R01-HL085323]; [R01-HL088451]; [R01-HL071739] FX This research was supported by contracts N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168 and N01-HC-95169 from the National Heart, Lung, and Blood Institute and by grants UL1-TR-000040 and UL1-TR-001079 from National Center for Research Resources. In addition, adiposity assessments were supported by grants R01-HL085323 (to JD), R01-HL088451 (to MA), and R01-HL071739 (to MB). Dr. Shah is supported by an American Heart Association Fellow-to-Faculty Award. NR 39 TC 1 Z9 1 U1 2 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0939-4753 EI 1590-3729 J9 NUTR METAB CARDIOVAS JI Nutr. Metab. Carbiovasc. Dis. PD MAR PY 2016 VL 26 IS 3 BP 185 EP 193 DI 10.1016/j.numecd.2015.12.012 PG 9 WC Cardiac & Cardiovascular Systems; Endocrinology & Metabolism; Nutrition & Dietetics SC Cardiovascular System & Cardiology; Endocrinology & Metabolism; Nutrition & Dietetics GA DG0XV UT WOS:000371790300003 PM 26899879 ER PT J AU Aspinall, WP Cooke, RM Havelaar, AH Hoffmann, S Hald, T AF Aspinall, W. P. Cooke, R. M. Havelaar, A. H. Hoffmann, S. Hald, T. TI Evaluation of a Performance-Based Expert Elicitation: WHO Global Attribution of Foodborne Diseases SO PLOS ONE LA English DT Article ID JUDGMENT AB For many societally important science-based decisions, data are inadequate, unreliable or non-existent, and expert advice is sought. In such cases, procedures for eliciting structured expert judgments (SEJ) are increasingly used. This raises questions regarding validity and reproducibility. This paper presents new findings from a large-scale international SEJ study intended to estimate the global burden of foodborne disease on behalf of WHO. The study involved 72 experts distributed over 134 expert panels, with panels comprising thirteen experts on average. Elicitations were conducted in five languages. Performance-based weighted solutions for target questions of interest were formed for each panel. These weights were based on individual expert's statistical accuracy and informativeness, determined using between ten and fifteen calibration variables from the experts' field with known values. Equal weights combinations were also calculated. The main conclusions on expert performance are: (1) SEJ does provide a science-based method for attribution of the global burden of foodborne diseases; (2) equal weighting of experts per panel increased statistical accuracy to acceptable levels, but at the cost of informativeness; (3) performance-based weighting increased informativeness, while retaining accuracy; (4) due to study constraints individual experts' accuracies were generally lower than in other SEJ studies, and (5) there was a negative correlation between experts' informativeness and statistical accuracy which attenuated as accuracy improved, revealing that the least accurate experts drive the negative correlation. It is shown, however, that performance-based weighting has the ability to yield statistically accurate and informative combinations of experts' judgments, thereby offsetting this contrary influence. The present findings suggest that application of SEJ on a large scale is feasible, and motivate the development of enhanced training and tools for remote elicitation of multiple, internationally-dispersed panels. C1 [Aspinall, W. P.] Aspinall & Associates, Tisbury, England. [Aspinall, W. P.] Univ Bristol, Bristol, Avon, England. [Cooke, R. M.] Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA. [Cooke, R. M.] Delft Univ Technol, Delft, Netherlands. [Havelaar, A. H.] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands. [Havelaar, A. H.] Univ Florida, Gainesville, FL USA. [Havelaar, A. H.] Univ Utrecht, Utrecht, Netherlands. [Hoffmann, S.] ERS, USDA, Washington, DC USA. [Hald, T.] Tech Univ Denmark, DK-2800 Lyngby, Denmark. RP Cooke, RM (reprint author), Resources Future Inc, 1616 P St NW, Washington, DC 20036 USA.; Cooke, RM (reprint author), Delft Univ Technol, Delft, Netherlands. EM cooke@rff.org OI Aspinall, Willy/0000-0001-6014-6042 FU World Health Organization (WHO); Natural Environment Research Council (Consortium on Risk in the Environment: Diagnostics, Integration, Benchmarking, Learning and Elicitation - CREDIBLE) [NE/J017450/1]; Aspinall Associates FX This study was commissioned and paid for by the World Health Organization (WHO). WPA was supported in part at Bristol University by the Natural Environment Research Council (Consortium on Risk in the Environment: Diagnostics, Integration, Benchmarking, Learning and Elicitation - CREDIBLE; grant number NE/J017450/1). Aspinall & Associates provided support in the form of salary for author WPA, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the 'author contributions' section. NR 15 TC 0 Z9 0 U1 2 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 1 PY 2016 VL 11 IS 3 AR e0149817 DI 10.1371/journal.pone.0149817 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF5ZW UT WOS:000371434500060 PM 26930595 ER PT J AU Baumbach, J Pudake, RN Johnson, C Kleinhans, K Ollhoff, A Palmer, RG Bhattacharyya, MK Sandhu, D AF Baumbach, Jordan Pudake, Ramesh N. Johnson, Callie Kleinhans, Kaylin Ollhoff, Alexandrea Palmer, Reid G. Bhattacharyya, Madan K. Sandhu, Devinder TI Transposon Tagging of a Male-Sterility, Female-Sterility Gene, St8, Revealed that the Meiotic MER3 DNA Helicase Activity Is Essential for Fertility in Soybean SO PLOS ONE LA English DT Article ID ANTHOCYANIN PIGMENTATION; CROSSOVER FORMATION; UNSTABLE MUTATION; CROSSING-OVER; MUTANTS; ALIGNMENT; EXPRESSION; PROTEINS; SYNAPSIS; EXCISION AB The W4 locus in soybean encodes a dihydroflavonol-4-reductase (DFR2) that regulates pigmentation patterns in flowers and hypocotyl. The mutable w4-m allele that governs variegated flowers has arisen through insertion of a CACTA-type transposable element, Tgm9, in DFR2. In the w4-m line, reversion from variegated to purple flower indicates excision of Tgm9, and its insertion at a new locus. Previously, we have identified a male-sterile, female-sterile mutant among the selfed progenies of a revertant plant carrying only purple flowers. Co-segregation between Tgm9 and the sterility phenotype suggested that the mutant was generated by insertion of Tgm9 at the St8 locus. The transposon was localized to exon 10 of Glyma. 16G072300 that shows high identity to the MER3 DNA helicase involved in crossing over. Molecular analysis of fertile branches from two independent revertant plants confirmed precise excision of Tgm9 from the st8 allele, which restored fertility. In soybean, the gene is expressed in flower-buds, trifoliate leaves and stem. Phylogenetic analysis placed St8 in a clade with the Arabidopsis and rice MER3 suggesting that St8 is most likely the orthologous MER3 soybean gene. This study established the utility of Tgm9 in gene identification as well as in forward and reverse genetics studies. C1 [Baumbach, Jordan; Johnson, Callie; Kleinhans, Kaylin; Ollhoff, Alexandrea; Sandhu, Devinder] Univ Wisconsin, Dept Biol, Stevens Point, WI 54481 USA. [Baumbach, Jordan; Pudake, Ramesh N.; Palmer, Reid G.; Bhattacharyya, Madan K.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Sandhu, Devinder] USDA ARS, Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Pudake, Ramesh N.] Amity Univ Uttar Pradesh, Amity Inst Nanotechnol, Noida 201303, UP, India. RP Sandhu, D (reprint author), Univ Wisconsin, Dept Biol, Stevens Point, WI 54481 USA.; Bhattacharyya, MK (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA.; Sandhu, D (reprint author), USDA ARS, Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM mbhattac@iastate.edu; devinder.sandhu@ars.usda.gov FU University of Wisconsin Stevens Point University Personnel Development Committee Funds; University of Wisconsin Stevens Point Student Research Funds; UWSP University Personnel Development Committee Funds; UWSP Student Research Funds FX Devinder Sandhu was supported by University of Wisconsin Stevens Point University Personnel Development Committee Funds. Callie Johnson was supported by University of Wisconsin Stevens Point Student Research Funds.; This research project was supported by the UWSP University Personnel Development Committee Funds and UWSP Student Research Funds. NR 39 TC 0 Z9 0 U1 2 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 1 PY 2016 VL 11 IS 3 AR e0150482 DI 10.1371/journal.pone.0150482 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF5ZW UT WOS:000371434500155 PM 26930200 ER PT J AU Gotia, HT Munro, JB Knowles, DP Daubenberger, CA Bishop, RP Silva, JC AF Gotia, Hanzel T. Munro, James B. Knowles, Donald P. Daubenberger, Claudia A. Bishop, Richard P. Silva, Joana C. TI Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines SO PLOS ONE LA English DT Article ID APICAL MEMBRANE ANTIGEN-1; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; PLASMODIUM-FALCIPARUM; BABESIA-BIGEMINA; GENOME SEQUENCE; CARRIER STATE; CATTLE; BUFFALOS; INVASION AB Theileria parva is a tick-transmitted intracellular apicomplexan pathogen of cattle in sub-Saharan Africa that causes East Coast fever (ECF). ECF is an acute fatal disease that kills over one million cattle annually, imposing a tremendous burden on African small-holder cattle farmers. The pathology and level of T. parva infections in its wildlife host, African buffalo (Syncerus caffer), and in cattle are distinct. We have developed an absolute quantification method based on quantitative PCR (qPCR) in which recombinant plasmids containing single copy genes specific to the parasite (apical membrane antigen 1 gene, ama1) or the host (hypoxanthine phosphoribosyltransferase 1, hprt1) are used as the quantification reference standards. Our study shows that T. parva and bovine cells are present in similar numbers in T. parva-infected lymphocyte cell lines and that consequently, due to its much smaller genome size, T. parva DNA comprises between 0.9% and 3% of the total DNA samples extracted from these lines. This absolute quantification assay of parasite and host genome copy number in a sample provides a simple and reliable method of assessing T. parva load in infected bovine lymphocytes, and is accurate over a wide range of host-to-parasite DNA ratios. Knowledge of the proportion of target DNA in a sample, as enabled by this method, is essential for efficient high-throughput genome sequencing applications for a variety of intracellular pathogens. This assay will also be very useful in future studies of interactions of distinct host-T. parva stocks and to fully characterize the dynamics of ECF infection in the field. C1 [Gotia, Hanzel T.; Munro, James B.; Silva, Joana C.] Univ Maryland, Sch Med, Inst Genome Sci, 801 West Baltimore St, Baltimore, MD USA. [Knowles, Donald P.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Knowles, Donald P.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Daubenberger, Claudia A.] Swiss Trop & Publ Hlth Inst, Socinstr 57, CH-4002 Basel, Switzerland. [Daubenberger, Claudia A.] Univ Basel, Peterspl 1, CH-4003 Basel, Switzerland. [Bishop, Richard P.] Int Livestock Res Inst, POB 30709, Nairobi 00100, Kenya. [Silva, Joana C.] Univ Maryland, Sch Med, Dept Microbiol & Immunol, 685 West Baltimore St, Baltimore, MD 21201 USA. RP Silva, JC (reprint author), Univ Maryland, Sch Med, Inst Genome Sci, 801 West Baltimore St, Baltimore, MD USA.; Silva, JC (reprint author), Univ Maryland, Sch Med, Dept Microbiol & Immunol, 685 West Baltimore St, Baltimore, MD 21201 USA. EM jcsilva@som.umaryland.edu RI Munro, James/C-5489-2009 OI Munro, James/0000-0003-3067-6434 FU CGIAR Consortium [CRP3.7]; United States Department of Agriculture, Agricultural Research Service [58-5348-4-013]; USAID [PASA - AID-BFS-P-13-00002, PASA-AID-BFS-P-13-00002] FX RPB was partly funded by CGIAR Consortium Research Project More Meat, Fish and Livestock by and for the poor (CRP3.7). This work was partly supported by the United States Department of Agriculture, Agricultural Research Service (Scientific Cooperative Agreement #58-5348-4-013 to JCS), and by USAID (PASA - AID-BFS-P-13-00002, Collaboration to Advance Vaccine Control of East Coast Fever, to DPK). The contents of this study are the sole responsibility of authors and do not necessarily reflect the views of the United States Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank Matthew Adams, from the University of Maryland Institute for Global Health, for his methodological guidance, Roger Pelle and Elias Awino, from ILRI, for providing DNA from T. parva-infected bovine lymphocyte cell lines, and Curt Van Tassell and Tad Sonstegard, from the USDA, Agricultural Research Service, in Beltsville, Maryland, for providing Bos taurus gDNA. RPB was partly funded by CGIAR Consortium Research Project More Meat, Fish and Livestock by and for the poor (CRP3.7). This work was partly supported by the United States Department of Agriculture, Agricultural Research Service (Scientific Cooperative Agreement #58-5348-4-013 to JCS), and by USAID (PASA-AID-BFS-P-13-00002, Collaboration to Advance Vaccine Control of East Coast Fever, to DPK). The contents of this study are the sole responsibility of authors and do not necessarily reflect the views of the United States Government. NR 39 TC 1 Z9 1 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 1 PY 2016 VL 11 IS 3 AR e0150401 DI 10.1371/journal.pone.0150401 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF5ZW UT WOS:000371434500141 PM 26930209 ER PT J AU McCaig, WD Loving, CL Hughes, HR Brockmeier, SL AF McCaig, William D. Loving, Crystal L. Hughes, Holly R. Brockmeier, Susan L. TI Characterization and Vaccine Potential of Outer Membrane Vesicles Produced by Haemophilus parasuis SO PLOS ONE LA English DT Article ID INNATE IMMUNE-RESPONSE; SERUM-RESISTANCE; SEROVAR 5; PSEUDOMONAS-AERUGINOSA; PROTECTIVE EFFICACY; PROTEOMIC ANALYSIS; SC096 STRAIN; PIGS; PROTEINS; INFLUENZAE AB Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structures has led to identification of virulence factors in a number of bacteria and they have been successfully used as subunit vaccines. We have isolated OMV from both virulent and avirulent strains of H. parasuis, have examined their protein content and assessed their ability to induce an immune response in the host. Vaccination with purified OMV derived from the virulent H. parasuis Nagasaki strain provided protection against challenge with a lethal dose of the bacteria. C1 [McCaig, William D.; Loving, Crystal L.; Hughes, Holly R.; Brockmeier, Susan L.] ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Brockmeier, SL (reprint author), ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM susan.brockmeier@ars.usda.gov FU U.S. Department of Energy; U.S. Department of Agriculture; DOE [DE-AC05-06OR23100] FX This research was supported in part by an appointment to the U.S. DEDA Agricultural Research Service Postdoctoral Research Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy and the U.S. Department of Agriculture. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 72 TC 5 Z9 6 U1 5 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 1 PY 2016 VL 11 IS 3 AR e0149132 DI 10.1371/journal.pone.0149132 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF5ZW UT WOS:000371434500016 PM 26930282 ER PT J AU Stockton, DG Martini, X Patt, JM Stelinski, LL AF Stockton, Dara G. Martini, Xavier Patt, Joseph M. Stelinski, Lukasz L. TI The Influence of Learning on Host Plant Preference in a Significant Phytopathogen Vector, Diaphorina citri SO PLOS ONE LA English DT Article ID PSYLLID HEMIPTERA LIVIIDAE; RHODNIUS-PROLIXUS STAL; ADULT ASIAN CITRUS; BLOOD 1ST EVIDENCE; MANDUCA-SEXTA; HUANGLONGBING PATHOGENS; LABORATORY CONDITIONS; BLOODSUCKING INSECT; INNATE PREFERENCES; COLOR PREFERENCES AB Although specialist herbivorous insects are guided by innate responses to host plant cues, host plant preference may be influenced by experience and is not dictated by instinct alone. The effect of learning on host plant preference was examined in the Asian citrus psyllid, Diaphorina citri; vector of the causal agent of citrus greening disease or huanglongbing. We investigated: a) whether development on specific host plant species influenced host plant preference in mature D. citri; and b) the extent of associative learning in D. citri in the form of simple and compound conditioning. Learning was measured by cue selection in a 2-choice behavioral assay and compared to naive controls. Our results showed that learned responses in D. citri are complex and diverse. The developmental host plant species influenced adult host plant preference, with female psyllids preferring the species on which they were reared. However, such preferences were subject to change with the introduction of an alternative host plant within 24-48 hrs, indicating a large degree of experience-dependent response plasticity. Additionally, learning occurred for multiple sensory modalities where novel olfactory and visual environmental cues were associated with the host plant. However, males and females displayed differing discriminatory abilities. In compound conditioning tasks, males exhibited recognition of a compound stimulus alone while females were capable of learning the individual components. These findings suggest D. citri are dynamic animals that demonstrate host plant preference based on developmental and adult experience and can learn to recognize olfactory and visual host plant stimuli in ways that may be sex specific. These experience-based associations are likely used by adults to locate and select suitable host plants for feeding and reproduction and may suggest the need for more tailored lures and traps, which reflect region-specific cultivars or predominate Rutaceae in the area being monitored. C1 [Stockton, Dara G.; Martini, Xavier; Stelinski, Lukasz L.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL USA. [Patt, Joseph M.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL USA. RP Stockton, DG (reprint author), Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL USA. EM dara.stockton@ufl.edu FU University of Florida; Citrus Research and Development Foundation FX This research was funded by the University of Florida and the Citrus Research and Development Foundation to LLS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 73 TC 1 Z9 2 U1 9 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 1 PY 2016 VL 11 IS 3 AR e0149815 DI 10.1371/journal.pone.0149815 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF5ZW UT WOS:000371434500058 PM 26930355 ER PT J AU Gardner, NM Riley, RT Showker, JL Voss, KA Sachs, AJ Maddox, JR Gelineau-van Waes, JB AF Gardner, Nicole M. Riley, Ronald T. Showker, Jency L. Voss, Kenneth A. Sachs, Andrew J. Maddox, Joyce R. Gelineau-van Waes, Janee B. TI Elevated Nuclear and Cytoplasmic FTY720-Phosphate in Mouse Embryonic Fibroblasts Suggests the Potential for Multiple Mechanisms in FTY720-Induced Neural Tube Defects SO TOXICOLOGICAL SCIENCES LA English DT Article DE FTY720; S1P receptors; histone deacetylase inhibition; histone post-translational modification (PTM); ABC294640; sphingosine kinase ID SPHINGOSINE 1-PHOSPHATE RECEPTORS; VALPROIC ACID; IMMUNOSUPPRESSANT FTY720; HISTONE DEACETYLASE; EXPRESSION PATTERNS; SPINA-BIFIDA; FINGOLIMOD; GENE; INHIBITION; BIOMARKERS AB FTY720 (fingolimod) is a U.S. Food and Drug Administration-approved drug to treat relapsing remitting multiple sclerosis. FTY720 treatment in pregnant inbred LM/Bc mice results in approximately 60% of embryos having a neural tube defect (NTD). Sphingosine kinases (Sphk1, Sphk2) phosphorylate FTY720 in vivo to form the bioactive metabolite FTY720-1-phosphate (FTY720-P). Cytoplasmic FTY720-P is an agonist for 4 of the 5 sphingosine-1-phosphate (S1P) receptors (S1P(1, 3-5)) and can also act as a functional antagonist of S1P(1), whereas FTY720-P generated in the nucleus inhibits histone deacetylases (HDACs), leading to increased histone acetylation. This study demonstrates that treatment of LM/Bc mouse embryonic fibroblasts (MEFs) with FTY720 results in a significant accumulation of FTY720-P in both the cytoplasmic and nuclear compartments. Elevated nuclear FTY720-P is associated with decreased HDAC activity and increased histone acetylation at H3K18 and H3K23 in LM/Bc MEFs. Treatment of LM/Bc MEFs with FTY720 and a selective Sphk2 inhibitor, ABC294640, significantly reduces the amount of FTY720-P that accumulates in the nucleus. The data provide insight into the relative amounts of FTY720-P generated in the nuclear versus cytoplasmic subcellular compartments after FTY720 treatment and the specific Sphk isoforms involved. The results of this study suggest that FTY720-induced NTDs may involve multiple mechanisms, including: (1) sustained and/or altered S1P receptor activation and signaling by FTY720-P produced in the cytoplasm and (2) HDAC inhibition and histone hyperacetylation by FTY720-P generated in the nucleus that could lead to epigenetic changes in gene regulation. C1 [Gardner, Nicole M.; Sachs, Andrew J.; Maddox, Joyce R.; Gelineau-van Waes, Janee B.] Creighton Univ, Sch Med, Dept Pharmacol, Criss 3 Room 551,2500 Calif Plaza, Omaha, NE 68178 USA. [Riley, Ronald T.; Showker, Jency L.; Voss, Kenneth A.] USDA ARS, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. RP Gardner, NM (reprint author), Creighton Univ, Sch Med, Dept Pharmacol, Criss 3 Room 551,2500 Calif Plaza, Omaha, NE 68178 USA. EM nicolegardner@creighton.edu FU USDA-ARS [NP108, 6612-42000-012-00D]; Eunice Kennedy Shriver National Institute of Child Health & Human Development & NIH Office of the Director [RC4HD067971]; Nebraska Tobacco Settlement Biomedical Research Development Fund [LB692]; Nebraska Department of Health and Human Services-Division of Public Health Stem Cell Grant [2013-06] FX This work was supported by USDA-ARS NP108 in house project (6612-42000-012-00D); Eunice Kennedy Shriver National Institute of Child Health & Human Development & NIH Office of the Director (RC4HD067971); LB692 Nebraska Tobacco Settlement Biomedical Research Development Fund; and Nebraska Department of Health and Human Services-Division of Public Health Stem Cell Grant (2013-06). NR 38 TC 0 Z9 0 U1 1 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 EI 1096-0929 J9 TOXICOL SCI JI Toxicol. Sci. PD MAR PY 2016 VL 150 IS 1 BP 161 EP 168 DI 10.1093/toxsci/kfv321 PG 8 WC Toxicology SC Toxicology GA DF8NS UT WOS:000371615300014 PM 26719367 ER PT J AU Chaffee, BK Beck, AP Owston, MA Kumar, S Baze, WB Magden, ER Dick, EJ Lammey, M Abee, CR AF Chaffee, B. K. Beck, A. P. Owston, M. A. Kumar, S. Baze, W. B. Magden, E. R. Dick, E. J., Jr. Lammey, M. Abee, C. R. TI Spontaneous Reproductive Tract Lesions in Aged Captive Chimpanzees SO VETERINARY PATHOLOGY LA English DT Article DE biological aging; chimpanzee; genital organs; female; genital organs; male; mammary glands; pathology; leiomyoma; uterine ID PRIMORDIAL FOLLICLE NUMBER; PAN-TROGLODYTES; FEMALE CHIMPANZEES; NONHUMAN-PRIMATES; MENOPAUSAL STATUS; LIFE-SPAN; POSTMENOPAUSAL WOMEN; UTERINE LEIOMYOMATA; OVARIAN-FOLLICLES; WILD CHIMPANZEES AB Chimpanzees (Pan troglodytes) have served as an important model for studies of reproductive diseases and aging-related disorders in humans. However, limited information is available about spontaneously occurring reproductive tract lesions in aging chimpanzees. In this article, the authors present histopathologic descriptions of lesions identified in the reproductive tract, including the mammary gland, of 33 female and 34 male aged chimpanzees from 3 captive populations. The most common findings in female chimpanzees were ovarian atrophy, uterine leiomyoma, adenomyosis, and endometrial atrophy. The most common findings in male chimpanzees were seminiferous tubule degeneration and lymphocytic infiltrates in the prostate gland. Other less common lesions included an ovarian granulosa cell tumor, cystic endometrial hyperplasia, an endometrial polyp, uterine artery hypertrophy and mineralization, atrophic vaginitis, mammary gland inflammation, prostatic epithelial hyperplasia, dilated seminal vesicles, a sperm granuloma, and lymphocytic infiltrates in the epididymis. The findings in this study closely mimic changes described in the reproductive tract of aged humans, with the exception of a lack of malignant changes observed in the mammary gland and prostate gland. C1 [Chaffee, B. K.; Beck, A. P.; Baze, W. B.; Magden, E. R.; Abee, C. R.] Univ Texas MD Anderson Canc Ctr, Michale E Keeling Ctr Comparat Med & Res, Bastrop, TX 78602 USA. [Owston, M. A.; Dick, E. J., Jr.] Texas Biomed Res Inst, Southwest Natl Primate Res Ctr, San Antonio, TX USA. [Kumar, S.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. [Lammey, M.] Alamogordo Primate Facil, Alamogordo, NM USA. RP Chaffee, BK (reprint author), Univ Texas MD Anderson Canc Ctr, Michale E Keeling Ctr Comparat Med & Res, Bastrop, TX 78602 USA. EM bkchaffee@mdanderson.org OI Kumar, Shyamesh/0000-0001-9261-2263 FU NIH HHS [P51 OD011133] NR 95 TC 1 Z9 1 U1 1 U2 2 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 EI 1544-2217 J9 VET PATHOL JI Vet. Pathol. PD MAR PY 2016 VL 53 IS 2 BP 425 EP 435 DI 10.1177/0300985815620654 PG 11 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA DF8PO UT WOS:000371620500015 PM 26823448 ER PT J AU Sabanadzovic, S Wintermantel, WM Valverde, RA McCreight, JD Aboughanem-Sabanadzovic, N AF Sabanadzovic, Sead Wintermantel, William M. Valverde, Rodrigo A. McCreight, James D. Aboughanem-Sabanadzovic, Nina TI Cucumis melo endornavirus: Genome organization, host range and co-divergence with the host SO VIRUS RESEARCH LA English DT Article DE Endornavirus; Melon; Genome; Phylogenetics; Evolution ID DOUBLE-STRANDED-RNA; MULTIPLE SEQUENCE ALIGNMENT; CYTOPLASMIC MALE-STERILITY; ORYZA-SATIVA-ENDORNAVIRUS; BELL PEPPER ENDORNAVIRUS; MOLECULAR CHARACTERIZATION; PHASEOLUS-VULGARIS; NUCLEOTIDE-SEQUENCE; UNITED-STATES; VICIA-FABA AB A high molecular weight dsRNA was isolated from a Cucumis melo L. plant (referred to as 'CL01') of an unknown cultivar and completely sequenced. Sequence analyses showed that dsRNA is associated with an endornavirus for which a name Cucumis melo endornavirus (CmEV) is proposed. The genome of CmEV-CL01 consists of 15,078 nt, contains a single, 4939 codons-long ORF and terminates with a stretch of 10 cytosine residues. Comparisons of the putative CmEV-encoded polyprotein with available references in protein databases revealed a unique genome organization characterized by the presence of the following domains: viral helicase Superfamily 1 (Hel-1), three glucosyltransferases (doublet of putative capsular polysaccharide synthesis proteins and a putative C_28_Glycosyltransferase), and an RNA-dependent RNA polymerase (RdRp). The presence of three glycome-related domains of different origin makes the genome organization of CmEV unique among endornaviruses. Phylogenetic analyses of viral RdRp domains showed that CmEV belongs to a specific lineage within the family Endornaviridae made exclusively of plant-infecting endornaviruses. An RT-PCR based survey demonstrated high incidence of CmEV among melon germplasm accession (>87% of tested samples). Analyses of partial genome sequences of CmEV isolates from 26 different melon genotypes suggest fine-tuned virus adaptation and co-divergence with the host. Finally, results of the present study revealed that CmEV is present in plants belonging to three different genera in the family Cucurbitaceae. Such diverse host range is unreported for known endornaviruses and suggests a long history of CmEV association with cucurbits predating their speciation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sabanadzovic, Sead] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Wintermantel, William M.; McCreight, James D.] ARS, USDA, Salinas, CA 93905 USA. [Valverde, Rodrigo A.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Aboughanem-Sabanadzovic, Nina] Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39762 USA. RP Sabanadzovic, S (reprint author), Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. EM ss501@msstate.edu OI Sabanadzovic, Sead/0000-0002-2995-2633 FU Special Research Initiative Funds from Mississippi Agriculture and Forestry Experiment Station (SRI-MAFES); USDA National Institute of Food and Agriculture FX The authors would like to acknowledge USDA-ARS Plant Genetic Resources Conservation Unit and Dr. C.S. Kousik (USDA Vegetable Laboratory, Charleston, SC) for providing seeds of some of cucurbits used throughout this work, as well as Ms. L. Hladky, Ms. P. Fashing and Mr. M. Estrada (USDA-ARS, Salinas, CA) for technical support. Partial financial support within Special Research Initiative Funds from Mississippi Agriculture and Forestry Experiment Station (SRI-MAFES) to the first and last authors, and the USDA National Institute of Food and Agriculture to the first, third and last author is acknowledged. NR 61 TC 2 Z9 2 U1 3 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD MAR PY 2016 VL 214 BP 49 EP 58 DI 10.1016/j.virusres.2016.01.001 PG 10 WC Virology SC Virology GA DG1PI UT WOS:000371839600007 PM 26805037 ER PT J AU Labeda, DP AF Labeda, David P. TI Taxonomic evaluation of putative Streptomyces scabiei strains held in the ARS Culture Collection (NRRL) using multi-locus sequence analysis SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Actinobacteria; Systematics; Streptomycetaceae ID SP-NOV; COMMON SCAB; SP. NOV; DNA; SUBSTITUTIONS; PHYLOGENIES; POTATO AB Multi-locus sequence analysis has been demonstrated to be a useful tool for identification of Streptomyces species and was previously applied to phylogenetically differentiate the type strains of species pathogenic on potatoes (Solanum tuberosum L.). The ARS Culture Collection (NRRL) contains 43 strains identified as Streptomyces scabiei deposited at various times since the 1950s and these were subjected to multi-locus sequence analysis utilising partial sequences of the house-keeping genes atpD, gyrB, recA, rpoB and trpB. Phylogenetic analyses confirmed the identity of 17 of these strains as Streptomyces scabiei, 9 of the strains as the potato-pathogenic species Streptomyces europaeiscabiei and 6 strains as potentially new phytopathogenic species. Of the 16 other strains, 12 were identified as members of previously described non-pathogenic Streptomyces species while the remaining 4 strains may represent heretofore unrecognised non-pathogenic species. This study demonstrated the value of this technique for the relatively rapid, simple and sensitive molecular identification of Streptomyces strains held in culture collections. C1 [Labeda, David P.] ARS, Mycotoxin Prevent & Appl Microbiol Res, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Labeda, DP (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM David.Labeda@ars.usda.gov FU ARS [301] FX The able technical assistance of E. Basehoar in determining the house keeping gene sequences and A. McGovern in producing draft genome sequences is gratefully acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. DPL and the ARS Culture Collection CRIS project was supported by ARS National Program 301. NR 22 TC 2 Z9 2 U1 3 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD MAR PY 2016 VL 109 IS 3 BP 349 EP 356 DI 10.1007/s10482-015-0637-6 PG 8 WC Microbiology SC Microbiology GA DF3IJ UT WOS:000371237200001 PM 26739474 ER PT J AU Seymour, M Deiner, K Altermatt, F AF Seymour, Mathew Deiner, Kristy Altermatt, Florian TI Scale and scope matter when explaining varying patterns of community diversity in riverine metacommunities SO BASIC AND APPLIED ECOLOGY LA English DT Article DE Macroinvertebrates; Freshwater; Variance partitioning; Phylogenetic; Functional; Metacommunity phylogenetics ID DISSIMILARITY COEFFICIENTS; DENDRITIC CONNECTIVITY; BIODIVERSITY; DYNAMICS; ECOLOGY; FRAMEWORK; SELECTION; NETWORKS; MATRICES AB Large-scale species and genetic metacommunity patterns are influenced by variation in environmental factors and distance between communities, according to previous studies. However, these studies often used different measures to assess patterns of metacommunity diversity, distances between communities and grain sizes at which environmental variables arc measured. This hinders interpretations and generalizations of the underlying process that drive metacommunity diversity. We applied a synthetic and multi -analytical approach to identify general factors structuring the diversity of a large riverine metacommunity. Using complementing approaches we analyzed how distance, measured as Euclidean or topological distance, and environmental factors, assessed at different grain sizes, influenced different measures of metacommunity diversity (species richness, functional richness and phylogenetic diversity) of mayfly, stonefly and caddisfly species in a large river network (river Rhine, Switzerland). We found the amount of explained variation in species diversity was generally unaffected by grain size, but improved with the use of topological distance, compared to Euclidean distance. Variation in functional diversity was best explained by environmental factors at small grain sizes and topological distance. Variation in phylogenetic diversity was best explained when environmental variables were assessed at larger grain sizes and Euclidean distance was used. Overall, our results indicate that processes structuring metacommunity diversity may differ at the species, functional or phylogenetic level of the community, as recently postulated in the metacommunity phylogenetics approach. While such differences may hinder comparisons across studies using different methodologies, it offers opportunities to disentangle the structuring factors within metacommunities by applying multiple analytical approaches to the same dataset. C1 [Seymour, Mathew; Deiner, Kristy; Altermatt, Florian] Swiss Fed Inst Aquat Sci & Technol, Dept Aquat Ecol, Eawag, Uberlandstr 133, CH-8600 Dubendorf, Switzerland. [Seymour, Mathew] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. [Altermatt, Florian] Univ Zurich, Inst Evolutionary Biol & Environm Studies, Winterthurerstr 190, CH-8057 Zurich, Switzerland. RP Seymour, M (reprint author), Swiss Fed Inst Aquat Sci & Technol, Dept Aquat Ecol, Eawag, Uberlandstr 133, CH-8600 Dubendorf, Switzerland. EM Mat.Seymour@gmail.com FU Swiss National Science Foundation [31003A_135622, PP00P3_50698] FX The Swiss Federal Office for the Environment provided the BDM data. We thank the many people who conducted field and laboratory work within the biodiversity monitoring programme, and N. Martinez who gave us access to the data. We thank L. Boumans, J, Gattolliat and S. Rutschmann for providing COL sequence data and R, Siber, for her assistance in acquiring GIS data. We thank K. Hovemeyer, P.R. PeresNeto and three anonymous reviewers for their helpful and constructive comments. Funding was provided by the Swiss National Science Foundation, grants nr. 31003A_135622 and PP00P3_50698. NR 60 TC 3 Z9 3 U1 9 U2 24 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1439-1791 EI 1618-0089 J9 BASIC APPL ECOL JI Basic Appl. Ecol. PD MAR PY 2016 VL 17 IS 2 BP 134 EP 144 DI 10.1016/j.baac.2015.10.007 PG 11 WC Ecology SC Environmental Sciences & Ecology GA DF4JL UT WOS:000371313700005 ER PT J AU Olivier, HM Jenkins, JA Berhow, M Carter, J AF Olivier, Heather M. Jenkins, Jill A. Berhow, Mark Carter, Jacoby TI A Pilot Study Testing a Natural and a Synthetic Molluscicide for Controlling Invasive Apple Snails (Pomacea maculata) SO BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article DE Invasive species; Niclosamide; Pomacea; Tea seed ID BIOLOGICAL-CONTROL; CANALICULATA; NICLOSAMIDE; ECOSYSTEM; RICE AB Pomacea maculata (formerly P. insularum), an apple snail native to South America, was discovered in Louisiana in 2008. These snails strip vegetation, reproduce at tremendous rates, and have reduced rice production and caused ecosystem changes in Asia. In this pilot study snails were exposed to two molluscicides, a tea (Camellia sinensis) seed derivative (TSD) or niclosamide monohydrate (Pestanal(A (R)), 2',5-dichloro-4'-nitrosalicylanilide, CAS #73360-56-2). Mortality was recorded after exposure to high or low concentrations (0.03 and 0.015 g/L for TSD, 1.3 and 0.13 mg/L for niclosamide). The TSD induced 100 % mortality at both concentrations. Niclosamide caused 100 % and 17 % mortality at high and low concentrations respectively. These molluscicides were also tested on potential biocontrol agents, the red swamp crayfish (Procambarus clarkii) and redear sunfish (Lepomis microlophus). No crayfish mortalities occurred at either concentration for either chemical, but sunfish experienced 100 % mortality with TSD (0.03 g/L), and 21 % mortality with niclosamide (0.13 mg/L). C1 [Olivier, Heather M.; Jenkins, Jill A.; Carter, Jacoby] US Geol Survey, Wetland & Aquat Res Ctr, 700 Cajundome Blvd, Lafayette, LA 70506 USA. [Berhow, Mark] USDA ARS, 1815 N Univ St, Peoria, IL 61604 USA. RP Olivier, HM (reprint author), US Geol Survey, Wetland & Aquat Res Ctr, 700 Cajundome Blvd, Lafayette, LA 70506 USA. EM hbirdsong@usgs.gov FU USGS Invasive Species Program FX This project was supported in part by the USGS Invasive Species Program. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 33 TC 0 Z9 0 U1 8 U2 29 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0007-4861 EI 1432-0800 J9 B ENVIRON CONTAM TOX JI Bull. Environ. Contam. Toxicol. PD MAR PY 2016 VL 96 IS 3 BP 289 EP 294 DI 10.1007/s00128-015-1709-z PG 6 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DF3LD UT WOS:000371244600004 PM 26687501 ER PT J AU Kwon, JH Lee, DH Swayne, DE Noh, JY Yuk, SS Erdene-Ochir, TO Hong, WT Jeong, JH Jeong, S Gwon, GB Song, CS AF Kwon, Jung-Hoon Lee, Dong-Hun Swayne, David E. Noh, Jin-Yong Yuk, Seong-Su Erdene-Ochir, Tseren-Ochir Hong, Woo-Tack Jeong, Jei-Hyun Jeong, Sol Gwon, Gyeong-Bin Song, Chang-Seon TI Highly Pathogenic Avian Influenza A(H5N8) Viruses Reintroduced into South Korea by Migratory Waterfowl, 2014-2015 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID H5N1; BIRDS; SURVEILLANCE; POULTRY; JAPAN; CHINA AB Highly pathogenic avian influenza A(H5N8) viruses were isolated from migratory waterfowl in South Korea during fall 2014-winter 2015, a recurrence after initial introduction in winter 2014. These reappeared viruses were phylogenetically distinct from isolates circulating in poultry farms in South Korea. C1 [Kwon, Jung-Hoon; Noh, Jin-Yong; Yuk, Seong-Su; Erdene-Ochir, Tseren-Ochir; Hong, Woo-Tack; Jeong, Jei-Hyun; Jeong, Sol; Gwon, Gyeong-Bin; Song, Chang-Seon] Konkuk Univ, Seoul 143701, South Korea. [Lee, Dong-Hun; Swayne, David E.] USDA, Athens, GA USA. RP Song, CS (reprint author), Konkuk Univ, Coll Vet Med, Avian Dis Lab, 1 Hwayang Dong, Seoul 143701, South Korea. EM songcs@konkuk.ac.kr FU Korea Institute of Animal Disease Management Technology Development program [313016032WT011]; Ministry of Agriculture, Food and Rural Affairs, Republic of Korea; KIST open research program [2E25720-15-P030] FX This work was funded by the Korea Institute of Animal Disease Management Technology Development program (grant no. 313016032WT011), the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea; and was partially funded by KIST open research program (project no. 2E25720-15-P030). NR 15 TC 4 Z9 4 U1 2 U2 4 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD MAR PY 2016 VL 22 IS 3 BP 507 EP 510 DI 10.3201/eid2103.151006 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DF2RS UT WOS:000371192100020 PM 26890406 ER PT J AU Cohen, J Blinn, CE Boyle, KJ Holmes, TP Moeltner, K AF Cohen, Jed Blinn, Christine E. Boyle, Kevin J. Holmes, Thomas P. Moeltner, Klaus TI Hedonic Valuation with Translating Amenities: Mountain Pine Beetles and Host Trees in the Colorado Front Range SO ENVIRONMENTAL & RESOURCE ECONOMICS LA English DT Article DE Forest pests; Property values; Repeat-sales model; Wildland-urban interface ID SURFACE-WATER QUALITY; REPEAT SALES INDEX; CLIMATE-CHANGE; PROPERTY-VALUES; AIR-QUALITY; PRICE MODELS; IMPACTS; ALASKA AB In hedonic valuation studies the policy-relevant environmental quality attribute of interest is often costly to measure, especially under pronounced spatial and temporal variability. However, in many cases this attribute affects home prices and consumer preferences solely through its impact on a readily observable, spatially delineated, and time-invariant feature of the physical landscape. We label such a feature a "translating amenity." We show that under certain conditions changes in the marginal effect of such amenities on home values over time can be used to draw inference on the implicit price of the unobserved environmental quality of interest. We illustrate this approach in the context of a repeat-sales model and the recently intensified outbreak of the Mountain Pine Beetle in the Colorado Front Range. C1 [Cohen, Jed; Boyle, Kevin J.; Moeltner, Klaus] Virginia Tech, Dept Agr & Appl Econ, 208 Hutcheson Hall, Blacksburg, VA 24061 USA. [Blinn, Christine E.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Holmes, Thomas P.] USDA Forest Serv, Southern Res Stn, Res Triangle Pk, NC USA. RP Moeltner, K (reprint author), Virginia Tech, Dept Agr & Appl Econ, 208 Hutcheson Hall, Blacksburg, VA 24061 USA. EM jedcohen@vt.edu; cblinn@vt.edu; kjboyle@vt.edu; tholmes@fs.fed.us; moeltner@vt.edu FU Southern Research Station, USDA Forest Service FX We thank seminar participants at the 2013 Meetings of the W3133 Western Regional Science Project (Coeur d' Alene, ID, Feb. 27-March 2), and the US Airforce Academy, Colorado Springs (March 15, 2013), for insightful and stimulating comments. Funding provided by the Southern Research Station, USDA Forest Service, is gratefully acknowledged. NR 50 TC 1 Z9 1 U1 3 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0924-6460 EI 1573-1502 J9 ENVIRON RESOUR ECON JI Environ. Resour. Econ. PD MAR PY 2016 VL 63 IS 3 BP 613 EP 642 DI 10.1007/s10640-014-9856-y PG 30 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA DF3QO UT WOS:000371260300005 ER PT J AU Engeman, R Cattaruzza, R Cattaruzza, M Fischer, J AF Engeman, Richard Cattaruzza, Renate Cattaruzza, Marco Fischer, Justin TI Photographic estimation of wild boar damage to alpine grazing pastures in the Carpathian Mountains of central Romania SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Animal damage; Feral hog; GIS; Sus scrofa; Swine rooting; Wild pig ID FERAL SWINE DAMAGE; PLANT-COMMUNITIES; FLORIDA; HABITAT AB Observations of wild boar damage to alpine grazing pastures in Romania's Carpathian Mountains were collected using photographs of the slopes from vantage points. We mapped the rooted areas and then used GIS software to estimate the relative proportions of the total grazing areas visible in the photographs that were damaged by wild boar. The amounts of damage from our two demonstration pastures were 11.2 and 13.5 %. Pastures are rented for summer grazing with grazing density monitored. Wild boar damage essentially decreases the economic benefit received for the cost of the grazing rights. This paper appears to be the first documentation of the very direct costs to livestock owners from significant wild boar rooting within rented pastures. The photographic method we present provides a quick and efficient means to quantify damage to alpine grazing pastures and may have broad application for mountainous areas where swine damage (or other disturbance) occurs and there is sufficient visibility of the damaged habitat. C1 [Engeman, Richard; Fischer, Justin] USDA APHIS WS Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Cattaruzza, Renate; Cattaruzza, Marco] Heidelberg Univ, Inst Physiol & Pathophysiol, Neuenheimer Feld 326, D-69120 Heidelberg, Germany. RP Engeman, R (reprint author), USDA APHIS WS Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM richard.m.engeman@aphis.usda.gov NR 17 TC 0 Z9 0 U1 5 U2 10 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD MAR PY 2016 VL 23 IS 5 BP 4949 EP 4952 DI 10.1007/s11356-016-6051-4 PG 4 WC Environmental Sciences SC Environmental Sciences & Ecology GA DF2FE UT WOS:000371156100093 PM 26780054 ER PT J AU Soltani, A Kumar, A Mergoum, M Pirseyedi, SM Hegstad, JB Mazaheri, M Kianian, SF AF Soltani, Ali Kumar, Ajay Mergoum, Mohamed Pirseyedi, Seyed Mostafa Hegstad, Justin B. Mazaheri, Mona Kianian, Shahryar F. TI Novel nuclear-cytoplasmic interaction in wheat (Triticum aestivum) induces vigorous plants SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Alloplasmic; Nuclear-cytoplasm interaction; Epigenetic; Vigor; Wheat ID GENE-EXPRESSION; HYBRID SPECIATION; PLASMON ANALYSIS; GENOME; CHLOROPLAST; AEGILOPS; SEED; HYBRIDIZATION; POLYPLOIDY; INSIGHTS AB Interspecific hybridization can be considered an accelerator of evolution, otherwise a slow process, solely dependent on mutation and recombination. Upon interspecific hybridization, several novel interactions between nuclear and cytoplasmic genomes emerge which provide additional sources of diversity. The magnitude and essence of intergenomic interactions between nuclear and cytoplasmic genomes remain unknown due to the direction of many crosses. This study was conducted to address the role of nuclear-cytoplasmic interactions as a source of variation upon hybridization. Wheat (Triticum aestivum) alloplasmic lines carrying the cytoplasm of Aegilops mutica along with an integrated approach utilizing comparative quantitative trait locus (QTL) and epigenome analysis were used to dissect this interaction. The results indicate that cytoplasmic genomes can modify the magnitude of QTL controlling certain physiological traits such as dry matter weight. Furthermore, methylation profiling analysis detected eight polymorphic regions affected by the cytoplasm type. In general, these results indicate that novel nuclear-cytoplasmic interactions can potentially trigger an epigenetic modification cascade in nuclear genes which eventually change the genetic network controlling physiological traits. These modified genetic networks can serve as new sources of variation to accelerate the evolutionary process. Furthermore, this variation can synthetically be produced by breeders in their programs to develop epigenomic-segregating lines. C1 [Soltani, Ali; Kumar, Ajay; Pirseyedi, Seyed Mostafa; Hegstad, Justin B.; Mazaheri, Mona] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Mergoum, Mohamed] Univ Georgia, Dept Crop & Soil, 1109 Expt St, Griffin, GA 30223 USA. [Kianian, Shahryar F.] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kianian, SF (reprint author), Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM Shahryar.Kianian@ars.usda.gov OI Mazaheri, Mona/0000-0001-6319-3725 FU NSF-IOS [1361554]; Monsanto Beachell-Borlaug International Scholars program FX We would like to thank Professor S.S. Maan and Professor K. Tsunewaki for the decades of dedicated effort in the development of alloplasmic wheat lines and providing them for use in this study. We are also grateful for all the assistance received from members of the Wheat Germplasm Enhancement and the Hard Red Spring Wheat programs at NDSU. This research was supported by NSF-IOS 1361554 to S.F.K. and Monsanto Beachell-Borlaug International Scholars program supporting A.S. in his graduate studies. NR 48 TC 0 Z9 0 U1 2 U2 7 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X EI 1438-7948 J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD MAR PY 2016 VL 16 IS 2 BP 171 EP 182 DI 10.1007/s10142-016-0475-2 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA DF8OD UT WOS:000371616500006 PM 26860316 ER PT J AU Wang, YL Tian, LY Xiong, DG Klosterman, SJ Xiao, SX Tian, CM AF Wang, Yonglin Tian, Longyan Xiong, Dianguang Klosterman, Steven J. Xiao, Shuxiao Tian, Chengming TI The mitogen-activated protein kinase gene, VdHog1, regulates osmotic stress response, microsclerotia formation and virulence in Verticillium dahliae SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Verticillium dahliae; Hogl-type MAPK; Microsclerotia formation; Virulence; Osmotic stress ID SACCHAROMYCES-CEREVISIAE; HYPEROSMOTIC STRESS; MAP KINASE; FILAMENTOUS FUNGI; HYDROPHOBIN GENE; PATHOGEN; YEAST; HOG1; PATHWAYS; EXPRESSION AB The fungus Verticillium dahliae has gained worldwide notoriety as a destructive plant pathogen, causing vascular wilt diseases on diverse plant species. V. dahliae produces melanized resting bodies, known as microsclerotia, which can survive for 15 years in the soil, and are thus critically important in its disease cycle. However, the molecular mechanisms that underpin microsclerotia formation, survival, and germination remain poorly understood. In this study, we observed that deletion of VdHog1 (Delta VdHog1), encoding a homolog of a high-osmolarity glycerol (HOG) response mitogen-activated protein kinase, displayed decreased numbers of melanized microsclerotia in culture, heightened sensitivity to hyperosmotic stress, and increased resistance to the fungicide fludioxonil. Through RNA-Seq analysis, we identified 221 genes differentially expressed in the Delta VdHog1 strain. Interestingly, the expression levels of genes involved in melanin biosynthesis, as well as the hydrophobin gene VDH1, involved in the early stage of microsclerotia formation, were significantly decreased in the Delta VdHog1 strains relative to the wild-type expression levels. The Delta VdHog1 strains exhibited decreased virulence relative to the wild type strain on smoke tree seedlings. These results indicate that VdHog1 regulates hyperosmotic stress responses in V. dahliae, and establishes the Hogl-mediated pathway as a target to further probe the up- and downstream processes that regulate asexual development in this fungus. (C) 2016 Elsevier Inc. All rights reserved. C1 [Wang, Yonglin; Tian, Longyan; Xiong, Dianguang; Xiao, Shuxiao; Tian, Chengming] Beijing Forestry Univ, Coll Forestry, Key Lab Silviculture & Conservat, Minist Educ, Beijing, Peoples R China. [Klosterman, Steven J.] USDA ARS, Salinas, CA USA. RP Tian, CM (reprint author), Beijing Forestry Univ, Coll Forestry, Key Lab Silviculture & Conservat, Minist Educ, Beijing, Peoples R China. EM chengmt@bjfu.edu.cn FU Fundamental Research Funds for Central Universities [YX2015-11]; National Natural Science Foundation of China [31370013] FX The research was supported by the Fundamental Research Funds for the Central Universities (YX2015-11) and National Natural Science Foundation of China (31370013) to W.Y. NR 47 TC 4 Z9 4 U1 2 U2 12 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD MAR PY 2016 VL 88 BP 13 EP 23 DI 10.1016/j.fgb.2016.01.011 PG 11 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA DF6CS UT WOS:000371444000002 PM 26812120 ER PT J AU Kitchen, SG AF Kitchen, Stanley G. TI Climate and human influences on historical fire regimes (AD 1400-1900) in the eastern Great Basin (USA) SO HOLOCENE LA English DT Article DE anthropogenic fire; El Nino Southern Oscillation; fire history; fire seasonality; Pacific Decadal Oscillation; Palmer Drought Severity Index ID WESTERN UNITED-STATES; PONDEROSA PINE FORESTS; NORTHERN SIERRA-NEVADA; COLORADO FRONT RANGE; LAKE TAHOE BASIN; SOUTHERN-OSCILLATION; PACIFIC-NORTHWEST; TREE RECRUITMENT; MONTANE FORESTS; VARIABILITY AB High fire activity in western North America is associated with drought. Drought and fire prevail under negative El Nino Southern Oscillation (ENSO) and Pacific Decadal Oscillation (PDO) phases in the Southwest and with positive phases in the Northwest. Here, I infer climate effects on historic fire patterns in the geographically intermediate, eastern Great Basin and seek out evidence of human influence on reconstructed fire regimes. Surface fire chronologies were constructed for 10 sites using tree-ring-based fire scars. Regional (67) and local (247) fire years and no-fire (187) years were identified from 1400 to 1900 CE. I compared fire chronologies with indices of the Palmer Drought Severity Index (PDSI), ENSO, and PDO. Regionally, fires were significantly more common during drought and were associated with negative ENSO and positive-to-negative PDO transitions while no-fire years were associated with positive ENSO and negative-to-positive PDO transitions. Conditions were significantly wetter 2 years prior to regional fire years and drier 4 years prior to no-fire years, providing evidence that fires were historically fuel limited. Local fire years occurred under a broad range of climate conditions. Most sites showed either persistent late or bimodal (early and late) fire seasonality patterns. These patterns are distinct from the mid-season peak observed for modern lightning-caused fires, suggesting a human influence on historical ignition patterns. Results demonstrate that climate was an important synchronizer of fire at the regional scale and that locally fire regimes were the product of climate-regulated fuels and some combination of human and lightning ignition patterns. C1 [Kitchen, Stanley G.] US Forest Serv, Shrub Sci Lab, USDA, Rocky Mt Res Stn, 735 North 500 East, Provo, UT 84606 USA. RP Kitchen, SG (reprint author), US Forest Serv, Shrub Sci Lab, USDA, Rocky Mt Res Stn, 735 North 500 East, Provo, UT 84606 USA. EM skitchen@fs.fed.us FU US Joint Fire Sciences Program; USDA Forest Service; Fishlake National Forest and Rocky Mountain Research Station; Utah Bureau of Land Management; Rocky Mountain Tree-Ring Research FX This work was funded by the US Joint Fire Sciences Program; USDA Forest Service, Fishlake National Forest and Rocky Mountain Research Station; Utah Bureau of Land Management; and Rocky Mountain Tree-Ring Research. NR 76 TC 1 Z9 1 U1 5 U2 12 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0959-6836 EI 1477-0911 J9 HOLOCENE JI Holocene PD MAR PY 2016 VL 26 IS 3 BP 397 EP 407 DI 10.1177/0959683615609751 PG 11 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DF1XO UT WOS:000371133100006 ER PT J AU Murphy, D Wyborn, C Yung, L Williams, DR Cleveland, C Eby, L Dobrowski, S Towler, E AF Murphy, Daniel Wyborn, Carina Yung, Laurie Williams, Daniel R. Cleveland, Cory Eby, Lisa Dobrowski, Solomon Towler, Erin TI Engaging Communities and Climate Change Futures with Multi-Scale, Iterative Scenario Building (MISB) in the Western United States SO HUMAN ORGANIZATION LA English DT Article DE climate change; adaptation; vulnerability; narrative; scenarios; uncertainty ID CHANGE ADAPTATION; ANTHROPOLOGY; WORLD; RESILIENCE; FRAMEWORK; ECOLOGY; SYSTEMS AB Current projections of future climate change foretell potentially transformative ecological changes that threaten communities globally. Using two case studies from the United States Intermountain West, this article highlights the ways in which a better articulation between theory and methods in research design can generate proactive applied tools that enable locally grounded dialogue about the future, including key vulnerabilities and potential adaptive pathways. Moreover, anthropological knowledge and methods, we find, are well-suited to the complexities and uncertainties that surround future climate change. In this article, we outline a narrative-driven assessment methodology we call multi-scale, iterative scenario building (MISB) that adheres to four key principles: (1) meaningful integration of socioecological interactions, (2) engagement with uncertainty, (3) awareness and incorporation of dynamic spatial and temporal scales, and (4) inclusion of diverse knowledge(s) from both social and natural sciences as well as from communities, including skeptics and deniers. The research found that MISB illuminated the complex, relational nature of vulnerability and adaptation and provided significant insight into potential, and sometimes surprising, future conflicts, synergies, and opportunities. We also found that MISB engendered a deep appreciation among participants, even skeptics and deniers, about the numerous, multi-scaled feedbacks and path dependencies generated by interacting drivers of social and ecological change. In conclusion, we argue this approach provides substantial space for the reflexive learning needed to create the "critical emancipatory knowledge" required in the face of transformational threats like climate change, and as such, we suggest potential avenues to support planning a nd decision making in the face of uncertain futures. C1 [Murphy, Daniel] Univ Cincinnati, Dept Anthropol, Cincinnati, OH 45221 USA. [Wyborn, Carina] World Wildlife Fund, Luc Hoffman Inst, Place Based Conservat, Gland, Switzerland. [Yung, Laurie; Cleveland, Cory; Eby, Lisa; Dobrowski, Solomon] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Williams, Daniel R.] US Forest Serv, Org Learning & Resiliency, Rocky Mt Res Stn, Ft Collins, CO USA. [Towler, Erin] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. RP Murphy, D (reprint author), Univ Cincinnati, Dept Anthropol, Cincinnati, OH 45221 USA.; Wyborn, C (reprint author), World Wildlife Fund, Luc Hoffman Inst, Place Based Conservat, Gland, Switzerland.; Yung, L; Cleveland, C; Eby, L; Dobrowski, S (reprint author), Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA.; Williams, DR (reprint author), US Forest Serv, Org Learning & Resiliency, Rocky Mt Res Stn, Ft Collins, CO USA.; Towler, E (reprint author), Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. FU USDA Forest Service Rocky Mountain Research Station; Montana Institute on Ecosystems (NSF EPSCoR) [NSF-IIA-1443108] FX The research was supported by the USDA Forest Service Rocky Mountain Research Station and the Montana Institute on Ecosystems (NSF EPSCoR Track-1 NSF-IIA-1443108). Opinions, findings, conclusions, or recommendations expressed in this paper are those of the authors and do not necessarily reflect the views of the USDA or the National Science Foundation. The authors wish to thank the communities in Grand County, Colorado and Beaverhead County, Montana as well as members of various land management agencies who participated in this research. NR 53 TC 1 Z9 1 U1 2 U2 9 PU SOC APPLIED ANTHROPOLOGY PI OKLAHOMA CITY PA 3000 UNITED FOUNDERS BLVD, STE 148, OKLAHOMA CITY, OK 73112 USA SN 0018-7259 EI 1938-3525 J9 HUM ORGAN JI Hum. Organ. PD SPR PY 2016 VL 75 IS 1 BP 33 EP 46 PG 14 WC Anthropology; Social Sciences, Interdisciplinary SC Anthropology; Social Sciences - Other Topics GA DF3II UT WOS:000371237100004 ER PT J AU Hazir, S Shapiro-Ilan, DI Hazir, C Leite, LG Cakmak, I Olson, D AF Hazir, Selcuk Shapiro-Ilan, David I. Hazir, Canan Leite, Luis G. Cakmak, Ibrahim Olson, Dawn TI Multifaceted effects of host plants on entomopathogenic nematodes SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Biological control; Entomopathogenic nematodes; Helicoverpa zea; Host diet; Trophic relationships; Steinernema riobrave; Tomato; Eggplant; Tobacco ID NEOAPLECTANA-CARPOCAPSAE NEMATODA; DIAPREPES-ABBREVIATUS; PARASITIC NEMATODES; STEINERNEMA-CARPOCAPSAE; HELICOVERPA-ZEA; IMMUNE DEFENSE; HERBIVORE; ROOTS; SUSCEPTIBILITY; INFECTION AB The success of parasites can be impacted by multi-trophic interactions. Tritrophic interactions have been observed in parasite-herbivore-host plant systems. Here we investigate aspects of multi-trophic interactions in a system involving an entomopathogenic nematode (EPN), its insect host, and host plant. Novel issues investigated include the impact of tritrophic interactions on nematode foraging behavior, the ability of EPNs to overcome negative tritrophic effects through genetic selection, and interactions with a fourth trophic level (nematode predators). We tested infectivity of the nematode, Steinernema riobrave, to corn earworm larvae (Helicoverpa zea) in three host plants, tobacco, eggplant and tomato. Tobacco reduced nematode virulence and reproduction relative to tomato and eggplant. However, successive selection (5 passages) overcame the deficiency; selected nematodes no longer exhibited reductions in phenotypic traits. Despite the loss in virulence and reproduction nematodes, first passage S. riobrave was more attracted to frass from insects fed tobacco than insects fed on other host plants. Therefore, we hypothesized the reduced virulence and reproduction in S. riobrave infecting tobacco fed insects would be based on a self-medicating tradeoff, such as deterring predation. We tested this hypothesis by assessing predatory success of the mite Sancassania polyphyllae and the springtail Sinella curviseta on nematodes reared on tobacco-fed larvae versus those fed on greater wax moth, Galleria mellonella, tomato fed larvae, or eggplant fed larvae. No advantage was observed in nematodes derived from tobacco fed larvae. In conclusion, our results indicated that insect-host plant diet has an important effect on nematode foraging, infectivity and reproduction. However, negative host plant effects, might be overcome through directed selection. We propose that host plant species should be considered when designing biocontrol programs using EPNs. (C) 2016 Elsevier Inc. All rights reserved. C1 [Hazir, Selcuk] Adnan Menderes Univ, Fac Arts & Sci, Dept Biol, TR-09100 Aydin, Turkey. [Hazir, Selcuk; Shapiro-Ilan, David I.; Hazir, Canan; Leite, Luis G.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Hazir, Canan] Adnan Menderes Univ, Aydin Hlth Serv Vocat Sch, TR-09100 Aydin, Turkey. [Leite, Luis G.] APTA, Inst Biol, CP 70, BR-13001970 Campinas, SP, Brazil. [Cakmak, Ibrahim] Adnan Menderes Univ, Fac Agr, Dept Plant Protect, TR-09100 Aydin, Turkey. [Olson, Dawn] USDA ARS, CPRMU, Tifton, GA 31793 USA. RP Hazir, S (reprint author), Adnan Menderes Univ, Fac Arts & Sci, Dept Biol, TR-09100 Aydin, Turkey. EM Selcuk.Hazir@gmail.com; David.Shapiro@ars.usda.gov NR 53 TC 0 Z9 0 U1 7 U2 16 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAR PY 2016 VL 135 BP 53 EP 59 DI 10.1016/j.jip.2016.02.004 PG 7 WC Zoology SC Zoology GA DF6FG UT WOS:000371450700007 PM 26896698 ER PT J AU Yuan, Q Jordan, R Brlansky, RH Minenkova, O Hartung, J AF Yuan, Qing Jordan, Ramon Brlansky, Ronald H. Minenkova, Olga Hartung, John TI Development of single chain variable fragment (scFv) antibodies against surface proteins of 'Ca. Liberibacter asiaticus' SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Huanglongbing; HLB; Citrus greening; Phage display; Phagemid; scFv ID REAL-TIME PCR; XYLELLA-FASTIDIOSA; MONOCLONAL-ANTIBODIES; GREENING DISEASE; PHAGE DISPLAY; TWITCHING MOTILITY; IV PILI; CITRUS; BACTERIUM; PLANTS AB 'Candidatus Liberibacter asiaticus' is the causal agent of citrus huanglongbing, the most serious disease of citrus worldwide. We have developed and applied immunization and affinity screening methods to develop a primary library of recombinant single chain variable fragment (scFv) antibodies in an M13 vector, pKM19. The antibody population is enriched for antibodies that bind antigens of 'Ca. Liberibacter asiaticus'. The primary library has more than 10(7) unique antibodies and the genes that encode them. We have screened this library for antibodies that bind to specifically-chosen proteins that are present on the surface of 'Ca. Liberibacter asiaticus'. These proteins were used as targets for affinity-based selection of scFvs that bind to the major outer membrane protein, OmpA; the polysaccharide capsule protein KpsF; a protein component of the type IV pilus (CapF); and, two flagellar proteins FlhA and FlgI. These scFvs have been used in ELISA and dot blot assays against purified protein antigens and 'Ca. Liberibacter asiaticus' infected plant extracts. We have also recloned many of these scFvs into a plasmid expression vector designed for the production of scFvs. Screening of these scFvs was more efficient when phage-bound, rather than soluble scFvs, were used. We have demonstrated a technology to produce antibodies at will and against any protein target encoded by 'Ca. Liberibacter asiaticus'. Applications could include advanced diagnostic methods for huanglongbing and the development of immune labeling reagents for in planta applications. Published by Elsevier B.V. C1 [Yuan, Qing; Hartung, John] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Jordan, Ramon] USDA ARS, US Natl Arboretum, Beltsville, MD 20705 USA. [Brlansky, Ronald H.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Minenkova, Olga] Sigma Tau Pharmaceut, Rome, Italy. RP Hartung, J (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. NR 45 TC 2 Z9 2 U1 2 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD MAR PY 2016 VL 122 BP 1 EP 7 DI 10.1016/j.mimet.2015.12.015 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DF5CA UT WOS:000371368200001 PM 26744234 ER PT J AU Yeh, HY Serrano, KV Acosta, AS Buhr, RJ AF Yeh, Hung-Yueh Serrano, Katherine Vargas Acosta, Aimee Silvestry Buhr, Richard J. TI Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Salmonella enterica; FlgK; Flagellar proteins; Zoonoses; Simple Western ID ENTERITIDIS INFECTION; UNITED-STATES; WESTERN-BLOT; LAYING HENS; PURIFICATION; TYPHIMURIUM; PATHOGENS; FOOD; ANTIGENICITY; QUANTITATION AB Conventional immunoblot assays are a useful tool for specific protein identification, but tedious, labor-intensive and time-consuming. A capillary electrophoresis-based immunoblot assay so-called "Simple Western" was developed to enable the protein identification in an automatic manner. This communication describes the use of Simple Western for detecting anti-Salmonella FlgK antibodies from chicken sera. Published by Elsevier B.V. C1 [Yeh, Hung-Yueh; Buhr, Richard J.] ARS, USDA, US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. [Serrano, Katherine Vargas; Acosta, Aimee Silvestry] Univ Puerto Rico, Fac Agr Sci, Food Sci & Technol Program, Mayaguez Campus,POB 9000, Mayaguez, PR 00681 USA. RP Yeh, HY (reprint author), ARS, USDA, US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hungyueh.yeh@ars.usda.gov FU USDA National Institute of Food and Agriculture from University of Puerto Rico, Mayaguez, Puerto Rico; USDA Agricultural Research Service CRIS Project [6612-32000-060-00]; U.S. Poultry & Egg Association Project [BRF002] FX We thank Susan Q Brooks of Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA for the technical support. Aimee Silvestry Acosta and Katherine Vargas Serrano were research interns of the RISE - Pathways to Diversity in Food Science Careers project, funded by the USDA National Institute of Food and Agriculture, from the University of Puerto Rico, Mayaguez, Puerto Rico.; This study was supported by the USDA Agricultural Research Service CRIS Project No. 6612-32000-060-00, and the U.S. Poultry & Egg Association Project No. BRF002. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture, which is an equal opportunity provider and employer. NR 35 TC 0 Z9 0 U1 2 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD MAR PY 2016 VL 122 BP 27 EP 32 DI 10.1016/j.mimet.2016.01.007 PG 6 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DF5CA UT WOS:000371368200007 PM 26784737 ER PT J AU Farhat, AE Sharma, S Abrams, SH Wong, WW Barlow, SE AF Farhat, Alicia Elena Sharma, Shreela Abrams, Stephanie H. Wong, William W. Barlow, Sarah E. TI Kamp K ' aana, a 2-Week Residential Weight Management Summer Camp, Shows Long-Term Improvement in Body Mass Index z Scores SO JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LA English DT Article DE childhood obesity; long-term outcome; obesity treatment; residential camp; weight loss ID OBESE CHILDREN; ADOLESCENTS; HEALTH; PROGRAM; BMI; OVERWEIGHT; REDUCTION; OUTCOMES AB Long-term effects of Kamp K'aana, a 2-week residential weight management camp, on body mass index (BMI) measures were evaluated on 71 of 108 (66%) obese youth 10 to 14 years of age. Measures were obtained at 11-month study follow-up (n=38) or extracted from medical record (n=33). Compared with baseline, BMI increased (P<0.001), but both BMI percentile and BMI z score decreased (98.7 +/- 1.0 to 97.3 +/- 6.7 and 2.34 +/- 0.30 to 2.23 +/- 0.34, P<0.001). A decrease in BMI z score of 0.2 units was seen in 27% of the participants (P<0.001). The short program has sustained effect. C1 [Farhat, Alicia Elena] North Texas Food Bank, Brighter Bites, Dallas, TX USA. [Sharma, Shreela] Univ Texas Houston, Sch Publ Hlth, Michael & Susan Dell Ctr Hlth Living, Houston, TX USA. [Abrams, Stephanie H.] Childrens Gastroenterol MCSG, Long Beach, CA USA. [Wong, William W.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. [Barlow, Sarah E.] Texas Childrens Hosp, Baylor Coll Med, 6701 Fannin St,Suite 1010, Houston, TX 77030 USA. RP Barlow, SE (reprint author), Texas Childrens Hosp, Baylor Coll Med, 6701 Fannin St,Suite 1010, Houston, TX 77030 USA. EM sbarlow@bcm.edu NR 14 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0277-2116 EI 1536-4801 J9 J PEDIATR GASTR NUTR JI J. Pediatr. Gastroenterol. Nutr. PD MAR PY 2016 VL 62 IS 3 BP 491 EP 494 DI 10.1097/MPG.0000000000000965 PG 4 WC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics SC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics GA DF4FS UT WOS:000371304000029 PM 26327212 ER PT J AU Ashby, RD Solaiman, DKY Liu, CK Strahan, G Latona, N AF Ashby, Richard D. Solaiman, Daniel K. Y. Liu, Cheng-Kung Strahan, Gary Latona, Nick TI Sophorolipid-Derived Unsaturated and Epoxy Fatty Acid Estolides as Plasticizers for Poly(3-Hydroxybutyrate) SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Polymers/coatings; Biobased products; Surfactants/detergents; Biobased products; Fermentation; Biotechnology biocatalysis; Chromatography; Lipid chemistry/lipid analysis; Spectroscopy; Lipid chemistry/lipid analysis; Thermal analysis; Lipid chemistry/lipid analysis ID PHYSICAL-PROPERTIES; OLEIC-ACID; ESTERS AB Unsaturated and epoxy fatty acid estolides were synthesized from the omega and omega-1 hydroxy fatty acids derived from sophorolipids (SL). These estolides were utilized as additives in solution-cast poly(3-hydroxybutyrate) (P3HB) films and their plasticizing effects reported. Estolides in the P3HB film matrix resulted in slight reductions of melting and glass transition temperatures while the crystallinity remained relatively constant (+/- 2.0 %). Scanning electron microscopy revealed irregular film surfaces in the presence of both estolides and the formation of pores within the P3HB film matrix. These irregularities influenced the tensile properties of the films by incrementally decreasing the tensile strength and moduli of the P3HB films and increasing their elongation. Curing the films for 3 months in the presence of estolide triggered an enhanced tensile strength and modulus when compared to the initial films. Irradiation was implemented in an attempt to crosslink the estolides. Results showed that radiation did not result in crosslinking but rather instigated chain scission and reduced the molecular weights by up to 81 % which further reduced the tensile strength, elongation and modulus of the P3HB films. These tensile property variations showed that while the tensile strength of the P3HB films declined in the presence of both estolides, the plasticity and elongation improved validating that these unique SL-derived estolides can be effectively used as plasticizers in P3HB films. C1 [Ashby, Richard D.; Solaiman, Daniel K. Y.; Liu, Cheng-Kung; Strahan, Gary; Latona, Nick] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Ashby, RD (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM rick.ashby@ars.usda.gov NR 32 TC 0 Z9 0 U1 2 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD MAR PY 2016 VL 93 IS 3 BP 347 EP 358 DI 10.1007/s11746-015-2772-7 PG 12 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DF3PR UT WOS:000371257600006 ER PT J AU Brandon, DL McKeon, TA Patfield, SA Kong, QL He, XH AF Brandon, David L. McKeon, Thomas A. Patfield, Stephanie A. Kong, Qiulian He, Xiaohua TI Analysis of Castor by ELISAs that Distinguish Ricin and Ricinus communis agglutinin (RCA) SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Oilseed; Ricin; Ricinus communis; Castor oil ID IN-GROUND BEEF; ASSAY; CONTAMINATION; ANTIBODIES; PROTEIN; MILK AB To facilitate the analysis of castor (Ricinus communis L.) seed fractions and germplasm for ricin content, we investigated the use of enzyme-linked immunosorbent assay (ELISA) methods to differentiate between ricin toxin and the related Ricinus communis agglutinin (RCA). Both proteins are based on a heterodimeric AB structure, with a common A chain. RCA comprises two dimers of A and B chains. Both proteins are found in the meal remaining after castor oil extraction and impede the commercial production of castor seed in the USA. We identified pairs of monoclonal antibodies (mAbs) that could distinguish between the structurally related proteins that share a common A chain. Antibody specificity was determined by ELISA and checked by immunoblotting. We found that mAb-mAb pairs afforded quantification of each castor protein, and that a mAb paired with a commercial polyclonal antibody provided detection of both with comparable sensitivity. C1 [Brandon, David L.; Patfield, Stephanie A.; Kong, Qiulian; He, Xiaohua] USDA ARS, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [McKeon, Thomas A.] USDA ARS, Bioprod Res Unit, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Brandon, DL (reprint author), USDA ARS, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM david.brandon@ars.usda.gov; xiaohua.he@ars.usda.gov FU Agricultural Research Service National Research Program [2030-42000-048]; National Research Program [2030-21410-021] FX We thank Uyen T. Tran and Anna M. Korn for assistance with initial antibody development. This work was conducted under Project 2030-42000-048 of Agricultural Research Service National Research Program 108 (Food Safety) and Project 2030-21410-021 of National Research Program 306 (Quality and Utilization of Agricultural Products). The exchange visit of Dr. Kong was supported by the Shanghai Shuneng Irradiation Technology Company, Shanghai, China. NR 17 TC 0 Z9 0 U1 4 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD MAR PY 2016 VL 93 IS 3 BP 359 EP 363 DI 10.1007/s11746-015-2773-6 PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DF3PR UT WOS:000371257600007 ER PT J AU Juarez, B AF Juarez, Bernadette TI A word from USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Juarez, Bernadette] USDA, APHIS, AC, Newtown Township, PA 19073 USA. RP Juarez, B (reprint author), USDA, APHIS, AC, Newtown Township, PA 19073 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD MAR PY 2016 VL 45 IS 3 BP 96 EP 96 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA DF2TQ UT WOS:000371197200017 PM 26886670 ER PT J AU Bowman, TA O'Keeffe, KR D'Aquila, T Yan, QW Griffin, JD Killion, EA Salter, DM Mashek, DG Buhman, KK Greenberg, AS AF Bowman, Thomas A. O'Keeffe, Kayleigh R. D'Aquila, Theresa Yan, Qing Wu Griffin, John D. Killion, Elizabeth A. Salter, Deanna M. Mashek, Douglas G. Buhman, Kimberly K. Greenberg, Andrew S. TI Acyl CoA synthetase 5 (ACSL5) ablation in mice increases energy expenditure and insulin sensitivity and delays fat absorption SO MOLECULAR METABOLISM LA English DT Article DE Dietary fat absorption; Acyl-CoA; ACSL; Intestine; Liver; FGF21 ID PPAR-ALPHA; LIPID-METABOLISM; SKELETAL-MUSCLE; ACID SYNTHESIS; MESSENGER-RNA; IN-VIVO; EXPRESSION; FGF21; RAT; TRIACYLGLYCEROL AB Objective: The family of acyl-CoA synthetase enzymes (ACSL) activates fatty acids within cells to generate long chain fatty acyl CoA (FACoA). The differing metabolic fates of FACoAs such as incorporation into neutral lipids, phospholipids, and oxidation pathways are differentially regulated by the ACSL isoforms. In vitro studies have suggested a role for ACSL5 in triglyceride synthesis; however, we have limited understanding of the in vivo actions of this ACSL isoform. Methods: To elucidate the in vivo actions of ACSL5 we generated a line of mice in which ACSL5 expression was ablated in all tissues (ACSL5 /). Results: Ablation of ACSL5 reduced ACSL activity by 80% in jejuna! mucosa, 50% in liver, and 37% in brown adipose tissue lysates. Body composition studies revealed that ACSL5 l, as compared to control ACSLexmc'xP, mice had significantly reduced fat mass and adipose fat pad weights. Indirect calorimetry studies demonstrated that ACSL5-1 had increased metabolic rates, and in the dark phase, increased respiratory quotient. In ACSL5/mice, fasting glucose and serum triglyceride were reduced; and insulin sensitivity was improved during an insulin tolerance test. Both hepatic mRNA (16 -fold) and serum levels of fibroblast growth factor 21 (FGF21) (-13-fold) were increased in ACSL5 `/ as compared to ACSLexm xP. Consistent with increased FGF21 serum levels, uncoupling protein -1 gene (Ucpl) and PPAR-gamma coactivator 1 alpha gene (Pgcla) transcript levels were increased in gonadal adipose tissue. To further evaluate ACSL5 function in intestine, mice were gavaged with an olive oil bolus; and the rate of triglyceride appearance in serum was found to be delayed in ACSL5/mice as compared to control mice. Conclusions: In summary, ACSL5-1 mice have increased hepatic and serum FGF21 levels, reduced adiposity, improved insulin sensitivity, increased energy expenditure and delayed triglyceride absorption. These studies suggest that ACSL5 is an important regulator of whole -body energy metabolism and ablation of ACSL5 may antagonize the development of obesity and insulin resistance. (C) 2015 The Authors. Published by Elsevier GmbH. This is an open access article under the CC-BY-NC-ND license (http//creativecommons.org/licenses/by-nc-nd/4.0/) C1 [Bowman, Thomas A.; O'Keeffe, Kayleigh R.; Yan, Qing Wu; Griffin, John D.; Killion, Elizabeth A.; Salter, Deanna M.; Greenberg, Andrew S.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Mashek, Douglas G.] Univ Minnesota, Dept Food Sci & Nutr, 1334 Eckles Ave, St Paul, MN USA. [D'Aquila, Theresa; Buhman, Kimberly K.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. RP Greenberg, AS (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM thormas.bowman@tufts.edu; kokeeffe@live.unc.edu; tdaquila@gmail.com; qing-wu.yan@tufts.edu; john.griffin@tufts.edu; elizabeth.killion@tufts.edu; deanna.salter@tufts.edu; dmashek@umn.edu; kbuhman@purdue.edu; andrew.greenberg@tufts.edu OI Mashek, Douglas/0000-0001-7033-3386 FU National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [F32-DK-095538]; Purdue Research Foundation; NIDDK [DK-0903634, R01 DK098606-02]; Minnesota Obesity Center [DK050456]; American Diabetes Association [7-13-IN-05]; Department of Defense, United States Army Medical Research Acquisition Activity [PC131237]; Indiana Clinical and Translational Sciences Institute from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award [UL1TR001108]; NIEHS [U01-ES-020958, R03-ES-0227]; NIDDK-Boston Nutrition Obesity Research Center [P30-DK-46200]; U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-70]; Robert C and Veronica Atkins endowed Professorship in Nutrition and Metabolism at Tufts Medical School; [T32 DK062032-24] FX T.A.B. received support from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (F32-DK-095538). T.D. receives support from the Purdue Research Foundation.; D.G.M. receives support from the NIDDK (DK-0903634) and the Minnesota Obesity Center (DK050456). K.K. B. receives support from the American Diabetes Association (7-13-IN-05), the Department of Defense, United States Army Medical Research Acquisition Activity (PC131237), and the Indiana Clinical and Translational Sciences Institute funded, in part by Grant Number Grant # UL1TR001108 from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award. A.S.G. receives support from NIEHS (U01-ES-020958, R03-ES-0227), NIDDK (R01 DK098606-02), NIDDK-Boston Nutrition Obesity Research Center (P30-DK-46200), T32 DK062032-24, and the U.S. Department of Agriculture, Agricultural Research Service, under agreement no. 58-1950-7-70. Dr. Greenberg is also the recipient of the Robert C and Veronica Atkins endowed Professorship in Nutrition and Metabolism at Tufts Medical School. NR 36 TC 3 Z9 3 U1 4 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-8778 J9 MOL METAB JI Mol. Metab. PD MAR PY 2016 VL 5 IS 3 BP 210 EP 220 DI 10.1016/j.molmet.2016.01.001 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DF2GT UT WOS:000371161300007 PM 26977393 ER PT J AU Lehrsch, GA Brown, B Lentz, RD Johnson-Maynard, JL Leytem, AB AF Lehrsch, G. A. Brown, B. Lentz, R. D. Johnson-Maynard, J. L. Leytem, A. B. TI Winter and growing season nitrogen mineralization from fall-applied composted or stockpiled solid dairy manure SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Dairy manure; Nitrogen recovery; Nitrogen use efficiency; Nutrient management; Organic fertilizer ID CATTLE FEEDLOT MANURE; SUGAR-BEET; CORN PRODUCTION; SOIL PROPERTIES; USE EFFICIENCY; N FLUXES; IN-SITU; AVAILABILITY; QUALITY; YIELD AB Adequate characterization of nitrogen (N) mineralization with time from manure and other organic sources is needed to maximize manure N use efficiency, decrease producer costs, and protect groundwater quality. The objective of our 2-year field study at Parma, ID, was to quantify in situ N mineralization with time as affected by a one-time fall application of solid dairy manure, either composted or stockpiled. The experiment included five treatments: a non-N fertilized control, two first-year rates of stockpiled solid dairy manure (21.9 and 43.8 Mg ha(-1), dry wt.) and two rates (53.1 and 106.1 Mg ha(-1), dry wt.) of composted dairy manure (hereafter termed compost). Net N mineralized (mineralization less immobilization) was determined to a depth of 0.3 m by repeatedly measuring soil inorganic N (NH4-N + NO3-N) concentrations in buried polyethylene bags. Overwinter mineralization was measured between amendment incorporation in fall and sugarbeet (Beta vulgaris L.) planting the following spring. In-season mineralization was measured in situ for seven consecutive incubation periods during the c. 220-day growing season for furrow-irrigated sugarbeet. Net N mineralized often varied among amendments and from year to year through mid-season, likely due to seasonal variation in soil temperature, annual differences in amendment properties, and other factors. In early spring 2003 after a warmer-than-normal winter, immobilization exceeded mineralization, regardless of treatment. In-season net N mineralized peaked between mid-August and early September (DOYs 230-251) each year, regardless of treatment. Annual (c. 11-month) net N mineralized in 2003 averaged 52 kg N ha(-1), similar among treatments. In 2004, annual net N mineralized was similar between rates within amendments and averaged 250 kg N ha(-1) where manure treated, 150 kg N ha(-1) where compost treated, and 106 kg N ha(-1) where untreated. On average in 2004, 31 % of compost's annual net N mineralized occurred before the growing season and 69 % during the season while essentially all of manure's net N mineralized occurred during the season. None of the amendments' total N was, in net, mineralized in 2003 but in 2004 on average, 2 % of compost's and 16 % of manure's total N was mineralized, similar between rates within amendments. When estimating annual net N mineralized from fall-applied organic amendments, one must account for abnormal temperatures, including those overwinter. C1 [Lehrsch, G. A.; Lentz, R. D.; Leytem, A. B.] USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. [Brown, B.] Univ Idaho, Parma Res & Extens Ctr, 29603 U 1 Lane, Parma, ID 83660 USA. [Johnson-Maynard, J. L.] Univ Idaho, PSES Agr Sci, Moscow, ID 83844 USA. RP Lehrsch, GA (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM Gary.Lehrsch@ars.usda.gov RI Johnson-Maynard, Jodi/E-9518-2013 NR 77 TC 1 Z9 1 U1 7 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 EI 1573-0867 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD MAR PY 2016 VL 104 IS 2 BP 125 EP 142 DI 10.1007/s10705-015-9755-9 PG 18 WC Soil Science SC Agriculture GA DF8WS UT WOS:000371641900002 ER PT J AU Rens, L Zotarelli, L Alva, A Rowland, D Liu, GD Morgan, K AF Rens, Libby Zotarelli, Lincoln Alva, Ashok Rowland, Diane Liu, Guodong Morgan, Kelly TI Fertilizer nitrogen uptake efficiencies for potato as influenced by application timing SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE N-15; Isotopes; Solanum tuberosum; Seepage irrigation; Florida ID SEEPAGE IRRIGATION; MARKETABLE YIELD; TRACER TECHNIQUE; SOIL-MOISTURE; QUALITY; FLORIDA; CROPS; MANAGEMENT; ATLANTIC; PLANT AB Estimation of N uptake efficiency of fertilizer applications at different growth stages of crop plants is critical to develop management recommendations that enhance fertilizer use by minimizing N losses. In this study, the N-fertilizer uptake efficiency (FNUE) of two chipping potato (Solanum tuberosum L.) varieties ('Atlantic' and 'FL1867') under three typical fertilizer application timings was investigated. All treatments received a total of 225 kg ha(-1) of N throughout the season, split into three applications of 75 kg ha(-1) as ammonium nitrate (NH4NO3) applied at pre-plant, plant emergence and tuber initiation. FNUE at each application timing was evaluated by the substitution of conventional N-fertilizer by isotope labeled ammonium nitrate ((NH)-N-15 (4) (15) NO3). Total tuber yield was similar between the two varieties at 48.8 and 37.5 Mg ha(-1) in 2013 and 2014, respectively. Likewise, the overall FNUE was also similar at 45 % across both varieties and years. FNUE was 11 % for the pre-plant application, while 62 % for the applications at emergence and tuber initiation stages. Since a small fraction of the N applied at pre-plant was recovered in the plant, N fertilizer application closer to the potato planting may increase the FNUE. C1 [Rens, Libby; Zotarelli, Lincoln; Liu, Guodong] Univ Florida, Dept Hort Sci, 1241 Fifield Hall, Gainesville, FL 32611 USA. [Alva, Ashok] USDA ARS, Sidney, MO USA. [Rowland, Diane] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Morgan, Kelly] Univ Florida, Soil & Water Sci Dept, Immokalee, FL USA. RP Zotarelli, L (reprint author), Univ Florida, Dept Hort Sci, 1241 Fifield Hall, Gainesville, FL 32611 USA. EM lzota@ufl.edu OI Zotarelli, Lincoln/0000-0002-1087-2043 NR 43 TC 0 Z9 0 U1 11 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 EI 1573-0867 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD MAR PY 2016 VL 104 IS 2 BP 175 EP 185 DI 10.1007/s10705-016-9765-2 PG 11 WC Soil Science SC Agriculture GA DF8WS UT WOS:000371641900005 ER PT J AU Helliwell, EE Vega-Arreguin, J Shi, Z Bailey, B Xiao, SY Maximova, SN Tyler, BM Guiltinan, MJ AF Helliwell, Emily E. Vega-Arreguin, Julio Shi, Zi Bailey, Bryan Xiao, Shunyuan Maximova, Siela N. Tyler, Brett M. Guiltinan, Mark J. TI Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE Theobroma cacao; disease resistance; phosphatidylinositol-3-phosphate-binding protein; effectors; oomycetes; fungi ID MEMBRANE DOCKING; EFFECTOR PROTEIN; PLANT; CELLS; PHYTOPHTHORA; DOMAIN; EXPRESSION; ENTRY; PHOSPHOINOSITIDES; LOCALIZATION AB The internalization of some oomycete and fungal pathogen effectors into host plant cells has been reported to be blocked by proteins that bind to the effectors' cell entry receptor, phosphatidylinositol-3-phosphate (PI3P). This finding suggested a novel strategy for disease control by engineering plants to secrete PI3P-binding proteins. In this study, we tested this strategy using the chocolate tree Theobroma cacao. Transient expression and secretion of four different PI3P-binding proteins in detached leaves of T.cacao greatly reduced infection by two oomycete pathogens, Phytophthora tropicalis and Phytophthora palmivora, which cause black pod disease. Lesion size and pathogen growth were reduced by up to 85%. Resistance was not conferred by proteins lacking a secretory leader, by proteins with mutations in their PI3P-binding site, or by a secreted PI4P-binding protein. Stably transformed, transgenic T.cacao plants expressing two different PI3P-binding proteins showed substantially enhanced resistance to both P.tropicalis and P.palmivora, as well as to the fungal pathogen Colletotrichum theobromicola. These results demonstrate that secretion of PI3P-binding proteins is an effective way to increase disease resistance in T.cacao, and potentially in other plants, against a broad spectrum of pathogens. C1 [Helliwell, Emily E.; Shi, Zi; Maximova, Siela N.; Guiltinan, Mark J.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Helliwell, Emily E.; Shi, Zi; Maximova, Siela N.; Guiltinan, Mark J.] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA. [Helliwell, Emily E.; Tyler, Brett M.] Oregon State Univ, Ctr Genome Res & Biocomputing, Corvallis, OR 97331 USA. [Helliwell, Emily E.; Tyler, Brett M.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Vega-Arreguin, Julio; Tyler, Brett M.] Virginia Polytech Inst & State Univ, Virginia Bioinformat Inst, Blacksburg, VA 24061 USA. [Vega-Arreguin, Julio; Tyler, Brett M.] Virginia Polytech Inst & State Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Bailey, Bryan] USDA ARS, Beltsville, MD 20705 USA. [Xiao, Shunyuan] Univ Maryland, Inst Biosci & Biotechnol Res, College Pk, MD 20742 USA. [Xiao, Shunyuan] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Vega-Arreguin, Julio] Univ Nacl Autonoma Mexico, ENES Unidad Leon, Leon 37684, Gto, Mexico. [Shi, Zi] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. RP Guiltinan, MJ (reprint author), Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. EM mjg9@psu.edu FU Pennsylvania State University, College of Agricultural Sciences; Huck Institutes of Life Sciences; American Research Institute Penn State Endowed Program in the Molecular Biology of Cacao; National Science Foundation BREAD Program [IOS-0965353]; National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-68004-30104] FX We would like to thank Lena Sheaffer and Sharon Pishak for the technical assistance in maintenance of our cacao tissue culture and transformation pipelines, German Sandoya for assistance with statistical analyses, Daniel McClosky for assistance with staining and microscopy procedures, Dylan Storey for advice on distinguishing P. tropicalis from P. capsici, and Brent Kronmiller for bioinformatics assistance. We are also grateful to Andrew Fister and Yufan Zhang for valuable comments throughout this project. This work was supported in part by The Pennsylvania State University, College of Agricultural Sciences, The Huck Institutes of Life Sciences, the American Research Institute Penn State Endowed Program in the Molecular Biology of Cacao and grants from the National Science Foundation BREAD Program (IOS-0965353) to BT, MG, SM and SX, and to BT from the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number (2011-68004-30104). NR 35 TC 1 Z9 1 U1 3 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD MAR PY 2016 VL 14 IS 3 BP 875 EP 886 DI 10.1111/pbi.12436 PG 12 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DF3DS UT WOS:000371224700005 PM 26214158 ER PT J AU Andrejczyk, K Butler, BJ Dickinson, BJ Hewes, JH Markowski-Lindsay, M Kittredge, DB Kilgore, MA Snyder, SA Catanzaro, PF AF Andrejczyk, Kyle Butler, Brett J. Dickinson, Brenton J. Hewes, Jaketon H. Markowski-Lindsay, Marla Kittredge, David B. Kilgore, Michael A. Snyder, Stephanie A. Catanzaro, Paul F. TI Family Forest Owners' Perceptions of Landowner Assistance Programs in the USA: A Qualitative Exploration of Program Impacts on Behaviour SO SMALL-SCALE FORESTRY LA English DT Article DE Focus groups; Landowner assistance programs; Forest Stewardship Program; Non industrial private forest owners ID STEWARDSHIP PROGRAM; INCENTIVE PROGRAMS; WEST-VIRGINIA; MANAGEMENT; MOTIVATIONS; MINNESOTA; FOCUS AB Using data collected from a series of focus groups, this study examines how landowner assistance programs (which may include management plans, cost-share, technical assistance and advice, and education components) affect family forest owner behaviour in the USA. Not surprisingly, most owners who participated in assistance programs had pre-existing management objectives. Participation in the management plan and cost-share components was found to facilitate the stewardship of private forests by assisting and reinforcing the behaviour of those landowners who already intend to manage their land in some pre-conceived manner. Advice and educational components appeared to do more in terms of introducing owners to new ideas. The mix of components offered as part of a landowner assistance program should consider the goals of the program and which components will be most effective in achieving those goals. C1 [Andrejczyk, Kyle; Dickinson, Brenton J.; Hewes, Jaketon H.; Markowski-Lindsay, Marla] Univ Massachusetts, Family Forest Res Ctr, Holdsworth Hall, Amherst, MA 01003 USA. [Butler, Brett J.] Univ Massachusetts, USDA, Forest Serv, No Res Stn, Holdsworth Hall, Amherst, MA 01003 USA. [Kittredge, David B.; Catanzaro, Paul F.] Univ Massachusetts, Dept Environm Conservat, Holdsworth Hall, Amherst, MA 01003 USA. [Kilgore, Michael A.] Univ Minnesota, Dept Forest Resources, Green Hall, St Paul, MN 55108 USA. [Snyder, Stephanie A.] USDA, Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. RP Butler, BJ (reprint author), Univ Massachusetts, USDA, Forest Serv, No Res Stn, Holdsworth Hall, Amherst, MA 01003 USA. EM bbutler01@fs.fed.us FU USDA [12-CS-11242305-062] FX The authors wish to express their sincere gratitude to the family forest owners who participated in this study and provided invaluable insights. This project was funded by a grant from the USDA Forest Service, State and Private Forestry, Cooperative Forestry Staff (Grant Number: 12-CS-11242305-062). NR 34 TC 0 Z9 0 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1873-7617 EI 1873-7854 J9 SMALL-SCALE FOR JI Small-Scale For. PD MAR PY 2016 VL 15 IS 1 BP 17 EP 28 DI 10.1007/s11842-015-9304-z PG 12 WC Forestry SC Forestry GA DF5TW UT WOS:000371416400002 ER PT J AU Sjolie, HK Becker, D Habesland, D Solberg, B Lindstad, BH Snyder, S Kilgore, M AF Sjolie, Hanne K. Becker, Dennis Habesland, Daniel Solberg, Birger Lindstad, Berit Hauger Snyder, Stephanie Kilgore, Mike TI Willingness of Nonindustrial Private Forest Owners in Norway to Supply Logging Residues for Wood Energy SO SMALL-SCALE FORESTRY LA English DT Article DE Bioenergy; Family forest owners; Logistic regression; Factor analysis ID LANDOWNER WILLINGNESS; MULTIPLE IMPUTATION; BIOMASS; BIOENERGY; AVAILABILITY; PREFERENCES; STATES; WORK AB Norway has set ambitious targets for increasing bioenergy production. Forest residue extraction levels are currently very low, but residues have the potential to be an important component of the wood energy supply chain. A representative sample of Norwegian nonindustrial private forest owners having at least 8 ha (20 acres) of productive forest land was surveyed about their willingness to supply logging residues for wood energy production. About 59 % responded that they were willing to do so. Logistic regression analyses revealed that the following factors were positively associated with the likelihood of being willing to supply logging residues: total forest area, education level, living in a region with active timber markets and a history of forest production, and having positive perceptions of residue extraction and forestry's role in mitigating climate change. Four variables were negatively associated with the likelihood to supply residues: living on property, being older than 65 years, having family or friends who are opposed to residue extraction, and having negative perceptions of residue extraction. The study provides insight regarding nonindustrial forest owners' attitudes towards extraction of forest residues that may aid policy-makers designing effective means to meet national bioenergy production goals. C1 [Sjolie, Hanne K.; Solberg, Birger; Lindstad, Berit Hauger] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, Box 5003, N-1432 As, Norway. [Becker, Dennis] Univ Idaho, Dept Nat Resources & Soc, 875 Perimeter Dr MS 1134, Moscow, ID 83844 USA. [Habesland, Daniel; Kilgore, Mike] Univ Minnesota, Dept Forest Resources, 1530 Cleveland Ave North, St Paul, MN 55108 USA. [Snyder, Stephanie] USDA, Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. RP Sjolie, HK (reprint author), Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, Box 5003, N-1432 As, Norway. EM hanne.sjolie@nmbu.no RI Becker, Dennis/E-6249-2017 FU Norwegian Centennial Chair Program; U.S. Department of State through the Fulbright Scholar Program; University of Minnesota's Agricultural Experiment Station; USDA Forest Service, Northern Research Station FX Funding for this project was provided in part by the Norwegian Centennial Chair Program, which is a joint research and academic venture between the Norwegian University of Life Sciences, University of Oslo, and the University of Minnesota. Partial funding was also provided by the U.S. Department of State through the Fulbright Scholar Program and the University of Minnesota's Agricultural Experiment Station. In-kind support was provided by the USDA Forest Service, Northern Research Station. All support is greatly appreciated. NR 46 TC 1 Z9 1 U1 1 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1873-7617 EI 1873-7854 J9 SMALL-SCALE FOR JI Small-Scale For. PD MAR PY 2016 VL 15 IS 1 BP 29 EP 43 DI 10.1007/s11842-015-9306-x PG 15 WC Forestry SC Forestry GA DF5TW UT WOS:000371416400003 ER PT J AU Ingersoll, GP Miller, DC Morris, KH McMurray, JA Port, G Caruso, BS AF Ingersoll, George P. Miller, Debra C. Morris, Kristi H. McMurray, Jill A. Port, Garrett Caruso, Brian S. TI Changing Regional Emissions of Airborne Pollutants Reflected in the Chemistry of Snowpacks and Wetfall in the Rocky Mountain Region, USA, 1993-2012 SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Atmospheric deposition; Snowpack; Rocky Mountains; Emissions ID WESTERN UNITED-STATES; REACTIVE NITROGEN; DEPOSITION; AMMONIA; COLORADO; SULFUR; TRENDS AB Wintertime precipitation sample data from 55 Snowpack sites and 17 National Atmospheric Deposition Program (NADP)/National Trends NetworkWetfall sites in the Rocky Mountain region were examined to identify long-term trends in chemical concentration, deposition, and precipitation using Regional and Seasonal Kendall tests. The Natural Resources Conservation Service snowtelemetry (SNOTEL) network provided snow-watere-quivalent data from 33 sites located near Snowpackand NADP Wetfall-sampling sites for further comparisons. Concentration and deposition of ammonium, calcium, nitrate, and sulfate were tested for trends for the period 1993-2012. Precipitation trends were compared between the three monitoring networks for the winter seasons and downward trends were observed for both Snowpack and SNOTEL networks, but not for the NADP Wetfall network. The dry-deposition fraction of total atmospheric deposition, relative to wet deposition, was shown to be considerable in the region. Potential sources of regional airborne pollutant emissions were identified from the U.S. Environmental Protection Agency 2011 National Emissions Inventory, and from longterm emissions data for the period 1996-2013. Changes in the emissions of ammonia, nitrogen oxides, and sulfur dioxide were reflected in significant trends in snowpack and wetfall chemistry. In general, ammonia emissions in the western USA showed a gradual increase over the past decade, while ammonium concentrations and deposition in snowpacks and wetfall showed upward trends. Emissions of nitrogen oxides and sulfur dioxide declined while regional trends in snowpack and wetfall concentrations and deposition of nitrate and sulfate were downward. C1 [Ingersoll, George P.; Port, Garrett; Caruso, Brian S.] US Geol Survey, Colorado Water Sci Ctr, Denver, CO 80225 USA. [Miller, Debra C.] US Forest Serv, Rocky Mountain Reg, Golden, CO 80401 USA. [Morris, Kristi H.] Natl Pk Serv, Air Resources Div, Denver, CO 80225 USA. [McMurray, Jill A.] US Forest Serv, Northern & Intermt Reg, Bozeman, MT 59715 USA. RP Caruso, BS (reprint author), US Geol Survey, Colorado Water Sci Ctr, Denver, CO 80225 USA. EM bcaruso@usgs.gov FU National Park Service; USDA Forest Service; Colorado Department of Public Health and Environment; Teton Conservation District; U.S. Geological Survey FX The authors greatly appreciate the funding support from the National Park Service, the USDA Forest Service, the Colorado Department of Public Health and Environment, the Teton Conservation District, and the U.S. Geological Survey. We also acknowledge the assistance of many individuals who contributed to this effort. In particular, we thank Ann Acheson, Nic Bencke, Gina Biere, Tamara Blett, Stan Bones, Mike Britten, Don Campbell, Michael Curtis, Liese Dean, Jay Dorr, Sam Duerksen, Thomas Dzomba, Dan Ely, Dan Fagre, Ben Glass, Bob Hammer, Mary Hektner, Nan Ingersoll, Joe Marcos, Craig McClure, Lisa McKeon, Greg Miller, David Mueller, Douglas Myhre, Rick Neam, Mark Nilles, Gary Nelson, Gordon Pierce, Ted Porwoll, Don Rosenberry, Orville Rosenberry, John Sacklin, Kevin Sage, Robb Sgroi, Craig Skeie, Jeff Sorkin, Ed Snook, Mark Story, Terry Svalberg, Kathy Tonnessen, John Turk, and Eric Winthers. Special thanks also is due to the generous logistical support from the following ski areas: The Big Mountain, Big Sky, Loveland, Showdown, Snowbowl, Taos Ski Valley, and Teton Village. Two anonymous reviewers provided useful comments. Dennis Cleary developed the emissions plots. Bob Larson compiled the NADP NTN data. NR 40 TC 0 Z9 0 U1 4 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD MAR PY 2016 VL 227 IS 3 AR 94 DI 10.1007/s11270-016-2784-4 PG 18 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA DF5XW UT WOS:000371427300024 ER PT J AU Cardoso, AS Berndt, A Leytem, A Alves, BJR de Carvalho, IDO Soares, LHD Urquiaga, S Boddey, RM AF Cardoso, Abmael S. Berndt, Alexandre Leytem, April Alves, Bruno J. R. de Carvalho, Isabel das N. O. de Barros Soares, Luis Henrique Urquiaga, Segundo Boddey, Robert M. TI Impact of the intensification of beef production in Brazil on greenhouse gas emissions and land use SO AGRICULTURAL SYSTEMS LA English DT Article DE Beef production; Brachiaria spp.; Brazil; Forage legume; Greenhouse gas emissions; Life-cycle analysis ID ATLANTIC FOREST REGION; SOIL CARBON STOCKS; NITROUS-OXIDE; BRACHIARIA PASTURES; METHANE EMISSIONS; NUTRITIVE-VALUE; CATTLE; FLUXES; AGRICULTURE; PARTITION AB Brazil has the largest herd of beef cattle in the world, estimated at approximately 200 million animals. Production is predominantly pasture-based and low input and hence time to slaughter is long, which promotes high methane (CH4) emissions per kg of product. The objective of this study was to investigate the impact of increasing animal productivity using fertilizers, forage legumes, supplements and concentrates, on the emissions of greenhouse gases (GHGs) in five scenarios for beef production in Brazil. A life cycle analysis (LCA) approach, from birth of calves to mature animals ready for slaughter at the farm gate, was utilized using Tier 2 methodologies of the IPCC and the results expressed in equivalents of carbon dioxide (CO(2)eq) per kg of carcass produced. Fossil CO2 emitted in the production of supplements, feeds and fertilizers was included using standard LCA techniques. The first four scenarios were based solely on cattle production on pasture, ranging from degraded Brachiaria pastures, through to a mixed legume/Brachiaria pasture and improved N-fertilized pastures of Guinea grass (Panicum maximum). Scenario 5 was the most intensive and was also based on an N-fertilized Guinea grass pasture, but with a 75-day finishing period in confinement with total mixed ration (TMR). Across the scenarios from 1 to 5 the increase in digestibility promoted a reduction in the forage intake per unit of animal weight gain and a concomitant reduction in CH4 emissions. For the estimation of nitrous oxide (N2O) emissions from animal excreta, emission factors from a study in the Cerrado region were utilized which postulated lower emission from dung than from urine and much lower emissions in the long dry season in this region. The greatest impact of intensification of the beef production systems was a 7-fold reduction of the area necessary for production from 320 to 45 m(2)/kg carcass. Carcass production increased from 43 to 65 Mg per herd across the scenarios from 1 to 5, and total emissions per kg carcass were estimated to be reduced from 58.3 to 29.4 kg CO(2)eq/kg carcass. Even though animal weight gain was lower in the mixed grass-legume scenario (3) than for the N-fertilized Guinea grass pastures (scenarios 4 and 5) GHG emissions per kg carcass were similar as the legume N-2 fixation input had no fossil-fuel cost. A large source of uncertainty for the construction of such LCAs was the lack of data for enteric CH4 emissions from cattle grazing tropical forages. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Cardoso, Abmael S.] UNESP, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, Brazil. [Berndt, Alexandre] Embrapa Pecuaria Sudeste, Rodovia Washington Luiz,Km 234, BR-13560970 Sao Carlos, SP, Brazil. [Leytem, April] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Alves, Bruno J. R.; de Carvalho, Isabel das N. O.; de Barros Soares, Luis Henrique; Urquiaga, Segundo; Boddey, Robert M.] Embrapa Agrobiol, Rodovia BR 465,Km 7, BR-23891000 Seropedica, RJ, Brazil. RP Boddey, RM (reprint author), Embrapa Agrobiol, Rodovia BR 465,Km 7, BR-23891000 Seropedica, RJ, Brazil. EM robert.boddey@embrapa.br OI Berndt, Alexandre/0000-0002-8976-2399 FU CAPES of Brazilian Ministry of Education; Embrapa; Brazilian National Research Council (CNPq); Rio de Janeiro State Research Foundation (FAPERJ) FX The author ASC would like to thank CAPES of Brazilian Ministry of Education for his MSc scholarship and the authors BJRA, SU and RMB thank Embrapa, the Brazilian National Research Council (CNPq) and the Rio de Janeiro State Research Foundation (FAPERJ) for scholarships and grants towards their work on GHG emissions from Agroecosystems. NR 62 TC 1 Z9 1 U1 19 U2 66 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-521X EI 1873-2267 J9 AGR SYST JI Agric. Syst. PD MAR PY 2016 VL 143 BP 86 EP 96 DI 10.1016/j.agsy.2015.12.007 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA DE8VL UT WOS:000370914700008 ER PT J AU DeGrandi-Hoffman, G Chen, YP Rivera, R Carroll, M Chambers, M Hidalgo, G de Jong, EW AF DeGrandi-Hoffman, Gloria Chen, Yanping Rivera, Raul Carroll, Mark Chambers, Mona Hidalgo, Geoffrey de Jong, Emily Watkins TI Honey bee colonies provided with natural forage have lower pathogen loads and higher overwinter survival than those fed protein supplements SO APIDOLOGIE LA English DT Article DE pollen nutrition; digestion; amino acids; Nosema; viruses ID POLLEN SUBSTITUTE DIETS; APIS-MELLIFERA L.; NOSEMA-CERANAE; NUTRITIONAL CONTENT; STORED POLLEN; UNITED-STATES; INFECTION; CONSUMPTION; MICROSPORIDIA; BROOD AB Malnutrition is a major cause of colony losses. In managed hives, bees are fed protein supplements (PS) during pollen shortages. If bees were provided with natural forage instead of PS, would they have lower pathogen levels and higher queen and colony survival? We addressed this question by either providing colonies with forage (Brassica rapa-rapini) or feeding them PS from November to February. Soluble protein concentrations in the PS were lower than the rapini pollen as were levels of most amino acids. Nurse bees digested less of the protein in PS than the pollen. Hemolymph protein titers in nurse bees and colony growth did not differ between those fed PS or foraging on rapini. However, colonies fed PS had higher levels of black queen cell virus and Nosema and greater queen losses, indicating that natural forage might improve overwintering survival. C1 [DeGrandi-Hoffman, Gloria; Rivera, Raul; Carroll, Mark; Chambers, Mona; Hidalgo, Geoffrey; de Jong, Emily Watkins] USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA. [Chen, Yanping] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. RP DeGrandi-Hoffman, G (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA. EM Gloria.Hoffman@ARS.USDA.GOV FU Almond Board of California FX The authors gratefully acknowledge Drs. Christina Grozinger and Amy Toth for their many helpful suggestions on earlier versions of this manuscript. We also thank the University of Arizona Agricultural Research Facilities for planting and caring for the rapini and the Almond Board of California for funding for this study. NR 46 TC 2 Z9 2 U1 12 U2 29 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAR PY 2016 VL 47 IS 2 BP 186 EP 196 DI 10.1007/s13592-015-0386-6 PG 11 WC Entomology SC Entomology GA DF2GV UT WOS:000371161500004 ER PT J AU Muzyka, D Pantin-Jackwood, M Starick, E Fereidouni, S AF Muzyka, Denys Pantin-Jackwood, Mary Starick, Elke Fereidouni, Sasan TI Evidence for genetic variation of Eurasian avian influenza viruses of subtype H15: the first report of an H15N7 virus SO ARCHIVES OF VIROLOGY LA English DT Article ID A VIRUSES; WILD BIRDS; NORTH-AMERICA; CODING REGION; SURVEILLANCE; SEQUENCES; SIBERIA; PROTEIN; SET AB Since the first detection of H15 avian influenza viruses (AIVs) in Australia in 1979, only seven H15 strains have been reported. A new H15 AIV was detected in Ukraine in 2010, carrying the unique HA-NA subtype combination H15N7. This virus replicated efficiently in chicken eggs, and antisera against it reacted strongly with the homologous antigen, but with lower titers when using the reference Australian antigen. The amino acid motifs of the HA cleavage site and receptor-binding site were different from those in the Australian viruses. The new virus, together with an H15 virus from Siberia from 2008, constitutes a new clade of H15 AIV isolates. C1 [Muzyka, Denys] Inst Expt & Clin Vet Med, Natl Sci Ctr, Kharkov, Ukraine. [Pantin-Jackwood, Mary] USDA, Agr Res Serv, Southeast Poultry Res Lab, Athens, GA USA. [Starick, Elke; Fereidouni, Sasan] Friedrich Loeffler Inst, Greifswald, Insel Riems, Germany. [Fereidouni, Sasan] WESCA Wildlife Network, Greifswald, Germany. [Fereidouni, Sasan] Univ Vet Med Vienna, Res Inst Wildlife Ecol, Vienna, Austria. RP Fereidouni, S (reprint author), Friedrich Loeffler Inst, Greifswald, Insel Riems, Germany.; Fereidouni, S (reprint author), WESCA Wildlife Network, Greifswald, Germany.; Fereidouni, S (reprint author), Univ Vet Med Vienna, Res Inst Wildlife Ecol, Vienna, Austria. EM sasan.fereidouni@vetmeduni.ac.at FU USDA project P444, through Ukrainian Science and Technology Center FX The authors gratefully acknowledge Tina Seehafer for the technical assistance, and the ornithologists from Azov-Black Sea Ornithological Station, especially Dr. R.M. Chernychko, for identification of birds' species and providing information on biological and ecological characteristics of wild birds from the Azov-Black Sea region of Ukraine. This research was funded partially by USDA project P444, through the Ukrainian Science and Technology Center. NR 21 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD MAR PY 2016 VL 161 IS 3 BP 605 EP 612 DI 10.1007/s00705-015-2629-2 PG 8 WC Virology SC Virology GA DF0SR UT WOS:000371049800011 PM 26650037 ER PT J AU Shriner, SA Root, JJ Mooers, NL Ellis, JW Stopak, SR Sullivan, HJ VanDalen, KK Franklin, AB AF Shriner, Susan A. Root, J. Jeffrey Mooers, Nicole L. Ellis, Jeremy W. Stopak, Scott R. Sullivan, Heather J. VanDalen, Kaci K. Franklin, Alan B. TI Susceptibility of rock doves to low-pathogenic avian influenza A viruses SO ARCHIVES OF VIROLOGY LA English DT Article ID DOMESTIC PIGEONS; H5N1; SUBTYPES; TRANSMISSION; ANTIBODIES; CHICKENS; LIVIA; ASSAY; H7 AB Following a 2008 outbreak of North American low-pathogenic H5N8 influenza A virus at an upland gamebird farm, we sero-sampled rock doves (pigeons, Columba livia) at the outbreak site and conducted experimental inoculations of wild-caught pigeons using the H5N8 virus and another low-pathogenic virus (H4N6). While 13 % of pigeons at the outbreak site were seropositive, none were positive for exposure to H5, and one was positive for N8. Challenged pigeons exhibited low susceptibility and limited viral RNA excretion for both viruses tested, but at least one individual had RNA loads indicative of the potential for viral transmission to other birds. C1 [Shriner, Susan A.; Root, J. Jeffrey; Mooers, Nicole L.; Ellis, Jeremy W.; Sullivan, Heather J.; VanDalen, Kaci K.; Franklin, Alan B.] Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Wildlife Serv, Ft Collins, CO 80521 USA. [Stopak, Scott R.] Anim & Plant Hlth Inspect Serv, Wildlife Serv, USDA, Boise, ID 83709 USA. RP Shriner, SA (reprint author), Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Wildlife Serv, Ft Collins, CO 80521 USA. EM Susan.A.Shriner@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182; Ellis, Jeremy/0000-0003-4512-0906 NR 24 TC 1 Z9 1 U1 1 U2 4 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD MAR PY 2016 VL 161 IS 3 BP 715 EP 720 DI 10.1007/s00705-015-2685-7 PG 6 WC Virology SC Virology GA DF0SR UT WOS:000371049800026 PM 26687583 ER PT J AU Wang, JW Zhu, DZ Tan, Y Zong, XJ Wei, HR Hammond, RW Liu, QZ AF Wang, Jiawei Zhu, Dongzi Tan, Yue Zong, Xiaojuan Wei, Hairong Hammond, Rosemarie W. Liu, Qingzhong TI Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China SO ARCHIVES OF VIROLOGY LA English DT Article ID 1ST REPORT; GENOME; CLOSTEROVIRUS; DISEASE AB Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China. C1 [Wang, Jiawei; Zhu, Dongzi; Tan, Yue; Zong, Xiaojuan; Wei, Hairong; Liu, Qingzhong] Shandong Inst Pomol, Key Lab Fruit Biotechnol Breeding Shandong Prov, Tai An 271000, Shandong, Peoples R China. [Hammond, Rosemarie W.] ARS, USDA, Mol Plant Pathol Lab, Room 214,Bldg 004 BARC West, Beltsville, MD 20705 USA. RP Liu, QZ (reprint author), Shandong Inst Pomol, Key Lab Fruit Biotechnol Breeding Shandong Prov, Tai An 271000, Shandong, Peoples R China.; Hammond, RW (reprint author), ARS, USDA, Mol Plant Pathol Lab, Room 214,Bldg 004 BARC West, Beltsville, MD 20705 USA. EM rose.hammond@ars.usda.gov; qzliu001@126.com OI Wang, Jiawei/0000-0002-5598-2115 FU Young Talents Training Program of Shandong Academy of Agricultural Sciences; International Science and Technology Cooperation Program of China [2012DFR30700]; Special Fund for Agro-Scientific Research in the Public Interest [201203075]; National Key Technology Research and Development Program of the Ministry of Science and Technology of China [2013BAD02B03-3-2]; Special Fund for Innovation Teams of Fruit Trees in Agricultural Technology System of Shandong Province [SDAIT-03-022-04] FX This work was supported by the Young Talents Training Program of Shandong Academy of Agricultural Sciences, the International Science and Technology Cooperation Program of China (2012DFR30700), the Special Fund for Agro-Scientific Research in the Public Interest (No. 201203075), National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2013BAD02B03-3-2) and Special Fund for Innovation Teams of Fruit Trees in Agricultural Technology System of Shandong Province (SDAIT-03-022-04). NR 17 TC 1 Z9 1 U1 1 U2 4 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD MAR PY 2016 VL 161 IS 3 BP 749 EP 753 DI 10.1007/s00705-015-2737-z PG 5 WC Virology SC Virology GA DF0SR UT WOS:000371049800033 PM 26733294 ER PT J AU Wienhold, BJ Schmer, MR Jin, VL Varvel, GE Gollany, H AF Wienhold, Brian J. Schmer, Marty R. Jin, Virginia L. Varvel, Gary E. Gollany, Hero TI CQESTR Simulated Changes in Soil Organic Carbon under Residue Management Practices in Continuous Corn Systems SO BIOENERGY RESEARCH LA English DT Article DE Maize; Nebraska; Feedstock; Soil quality; Irrigation; Tillage; N fertilization ID REMOVAL; TILLAGE; MATTER; MODEL; CROP; SEQUESTRATION; AGRICULTURE; BIOENERGY; DYNAMICS; MAIZE AB Soil organic carbon (SOC) is an important soil property and is strongly influenced by management. Changes in SOC stocks are difficult to measure through direct sampling, requiring both long time periods and intensive sampling to detect small changes in the large, highly variable pool. Models have the potential to predict management-induced changes in SOC stocks, but require long-term data sets for validation. CQESTR is a processed-based C model that uses site weather, management, and crop data to estimate changes in SOC stocks. Crop residue removal for livestock feed or future biofuel feedstock use is a management practice that potentially affects SOC stocks. Simulated changes in SOC using CQESTR were compared to measured SOC changes over 10 years for two contrasting residue removal studies in eastern Nebraska. The rainfed study compared SOC changes in no-tillage continuous corn grown under two N fertilizer rates (120 or 180 kg N ha(-1)) and two residue removal rates (0 or 50 %). The irrigated study compared SOC changes in continuous corn grown under no-tillage or disk tillage and three residue removal rates (0, 35, or 70 %). After 10 years under these management scenarios, CQESTR-estimated SOC stocks agreed well with the measured SOC stocks at both sites (r (2) = 0.93 at the rainfed site and r (2) = 0.82 at the irrigated site). These results are consistent with other CQESTR validation studies and demonstrate that this process-based model can be a suitable tool for supporting current management and long-term planning decisions. C1 [Wienhold, Brian J.; Schmer, Marty R.; Jin, Virginia L.] USDA ARS, Agroecosyst Management Res Unit, Rm 137 Keim Hall UNL East Campus, Lincoln, NE 68583 USA. [Varvel, Gary E.] UNL, Agroecosyst Management Res Unit, USDA ARS, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. [Gollany, Hero] USDA ARS, Columbia Plateau Conservat Res Ctr, POB 370, Pendleton, OR 97801 USA. RP Wienhold, BJ (reprint author), USDA ARS, Agroecosyst Management Res Unit, Rm 137 Keim Hall UNL East Campus, Lincoln, NE 68583 USA. EM brian.wienhold@ars.usda.gov NR 38 TC 2 Z9 2 U1 2 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2016 VL 9 IS 1 BP 23 EP 30 DI 10.1007/s12155-015-9654-6 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DE7KV UT WOS:000370816300003 ER PT J AU Mitchell, RB Vogel, KP AF Mitchell, R. B. Vogel, K. P. TI Grass Invasion into Switchgrass Managed for Biomass Energy SO BIOENERGY RESEARCH LA English DT Article DE Big bluestem(Andropogon gerardii); Bioenergy; Invasive species; Smooth bromegrass (Bromus inermis); Switchgrass (Panicum virgatum) ID BIOENERGY; INVASIVENESS; POPULATIONS; CALIFORNIA; BIOFUELS; PLANTS AB Switchgrass (Panicum virgatum) is a C-4 perennial grass and is the model herbaceous perennial bioenergy feedstock. Although it is indigenous to North American grasslands east of the Rocky Mountains and has been planted for forage and conservation purposes for more than 75 years, there is concern that switchgrass grown as a biofuel crop could become invasive. Our objective is to report on the invasion of C-4 and C-3 grasses into the stands of two switchgrass cultivars following 10 years of management for biomass energy under different N and harvest management regimes in eastern Nebraska. Switchgrass stands were invaded by big bluestem (Andropogon gerardii), smooth bromegrass (Bromus inermis), and other grasses during the 10 years. The greatest invasion by grasses occurred in plots to which 0 N had been applied and with harvests at anthesis. In general, less grass encroachment occurred in plots receiving at least 60 kg of N ha(-1) or in plots harvested after frost. There were differences among cultivars with Cave-in-Rock being more resistant to invasion than Trailblazer. There was no observable evidence of switchgrass from this study invading into border areas or adjacent fields after 10 years of management for biomass energy. Results indicate that switchgrass is more likely to be invaded by other grasses than to encroach into native prairies or perennial grasslands seeded on marginally productive crop-land in the western Corn Belt of the USA. C1 [Mitchell, R. B.; Vogel, K. P.] Univ Nebraska, Cent East Reg Biomass Res Ctr, Grain Forage & Bioenergy Res Unit, USDA Agr Res Serv, 251 Filley Hall Food Ind Complex, Lincoln, NE 68583 USA. RP Mitchell, RB (reprint author), Univ Nebraska, Cent East Reg Biomass Res Ctr, Grain Forage & Bioenergy Res Unit, USDA Agr Res Serv, 251 Filley Hall Food Ind Complex, Lincoln, NE 68583 USA. EM rob.mitchell@ars.usda.gov NR 23 TC 1 Z9 1 U1 2 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD MAR PY 2016 VL 9 IS 1 BP 50 EP 56 DI 10.1007/s12155-015-9656-4 PG 7 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DE7KV UT WOS:000370816300006 ER PT J AU Wickner, RB Kelly, AC AF Wickner, Reed B. Kelly, Amy C. TI Prions are affected by evolution at two levels SO CELLULAR AND MOLECULAR LIFE SCIENCES LA English DT Review DE Amyloid; Ure2p; Sup35p; HET-s; Rnq1; Parallel in-register beta sheet ID CHRONIC WASTING DISEASE; WHITE-TAILED DEER; HET-S PRION; BOVINE SPONGIFORM ENCEPHALOPATHY; BETA-SHEET STRUCTURE; CREUTZFELDT-JAKOB-DISEASE; 2-MU-M CIRCLE PLASMID; CHAIN RELEASE FACTOR; MESSENGER-RNA DECAY; SACCHAROMYCES-CEREVISIAE AB Prions, infectious proteins, can transmit diseases or be the basis of heritable traits (or both), mostly based on amyloid forms of the prion protein. A single protein sequence can be the basis for many prion strains/variants, with different biological properties based on different amyloid conformations, each rather stably propagating. Prions are unique in that evolution and selection work at both the level of the chromosomal gene encoding the protein, and on the prion itself selecting prion variants. Here, we summarize what is known about the evolution of prion proteins, both the genes and the prions themselves. We contrast the one known functional prion, [Het-s] of Podospora anserina, with the known disease prions, the yeast prions [PSI+] and [URE3] and the transmissible spongiform encephalopathies of mammals. C1 [Wickner, Reed B.; Kelly, Amy C.] NIDDK, Lab Biochem & Genet, NIH, Bldg 8,Room 225,8 Ctr Dr MSC 0830, Bethesda, MD 20892 USA. [Kelly, Amy C.] ARS, NCAUR, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Wickner, RB (reprint author), NIDDK, Lab Biochem & Genet, NIH, Bldg 8,Room 225,8 Ctr Dr MSC 0830, Bethesda, MD 20892 USA. EM wickner@helix.nih.gov; amy.kelly@ars.usda.gov FU National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health FX This work was supported in part by the Intramural Program of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health NR 165 TC 2 Z9 2 U1 12 U2 27 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 1420-682X EI 1420-9071 J9 CELL MOL LIFE SCI JI Cell. Mol. Life Sci. PD MAR PY 2016 VL 73 IS 6 BP 1131 EP 1144 DI 10.1007/s00018-015-2109-6 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA DF1DQ UT WOS:000371079200002 PM 26713322 ER PT J AU Li, JH Hunt, JF Gong, SQ Cai, ZY AF Li, Jinghao Hunt, John F. Gong, Shaoqin Cai, Zhiyong TI Improved fatigue performance for wood-based structural panels using slot and tab construction SO COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING LA English DT Article DE Biocomposite; Fatigue; Mechanical testing; Joints/joining ID HONEYCOMB SANDWICH BEAMS; STRENGTH; CELLULOSE; FIBERS; FOAM AB This paper presents static and fatigue bending behavior for a wood-based structural panel having a slot and tab (S/T) construction technique. Comparisons were made with similarly fabricated panels without the SIT construction technique. Experimental results showed that both types of panels had similar bending properties in the static tests. However, the panels with S/T construction had better fatigue results. The failure modes were different for the two fabrication techniques. The panels without SIT debonded at the core:face interface. Whereas, the panels with SIT had cracks that propagated within the rib of the core after debonding damage at the core:face interface. The fatigue deflection-life relationship indicated that the S/T construction improved the connection between the faces and core. The S/T construction decreased the deflection growth rate that delayed panel failure. The fatigue stress-life relationship or degradation was better for the panels with SIT construction than the panels without the S/T construction. (C) 2016 Published by Elsevier Ltd. C1 [Li, Jinghao; Gong, Shaoqin] Univ Wisconsin, Wisconsin Inst Discovery & Mat Sci & Engn, Dept Biomed Engn, Madison, WI 53715 USA. [Li, Jinghao; Hunt, John F.; Cai, Zhiyong] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Hunt, JF; Cai, ZY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jfhunt@fs.fed.us; zcai@fs.fed.us FU USDA Forest Service, Forest Products Laboratory FX This work is supported by USDA Forest Service, Forest Products Laboratory and the authors gratefully acknowledge the support of Sara Fishwild, James Bridwell, Marshall Begel, Dave Simpson and Marc Joyal of EMRSL group for the mechanical testing. NR 24 TC 3 Z9 3 U1 3 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1359-835X EI 1878-5840 J9 COMPOS PART A-APPL S JI Compos. Pt. A-Appl. Sci. Manuf. PD MAR PY 2016 VL 82 BP 235 EP 242 DI 10.1016/j.compositesa.2015.12.017 PG 8 WC Engineering, Manufacturing; Materials Science, Composites SC Engineering; Materials Science GA DE9YY UT WOS:000370997000024 ER PT J AU Boddu, VM Brenner, MW Patel, JS Kumar, A Mantena, PR Tadepalli, T Pramanik, B AF Boddu, Veera M. Brenner, Matthew W. Patel, Jignesh S. Kumar, Ashok Mantena, P. Raju Tadepalli, Tezeswi Pramanik, Brahmananda TI Energy dissipation and high-strain rate dynamic response of E-glass fiber composites with anchored carbon nanotubes SO COMPOSITES PART B-ENGINEERING LA English DT Article DE Mechanical properties; Glass fibres; Nano-structures; Mechanical testing ID BALLISTIC-PROTECTION PERFORMANCE; MECHANICAL-PROPERTIES; POLYMER COMPOSITES; EPOXY COMPOSITE; NANOCOMPOSITES; BEHAVIOR; ARMOR; SCIENCE; SURFACE AB This study explores the mechanical properties of an E-glass fabric composite reinforced with anchored multi-walled carbon nanotubes (CNTs). The CNTs were grown on the E-glass fabric using a floating catalyst chemical vapor deposition procedure. The E-glass fabric with attached CNTs was then incorporated into resin based composites and compared to similar composites without CNTs. Long and short beam bending tests, uniaxial compression measurements for energy dissipation, high strain-rate Split Hopkinson pressure bar measurements, and ballistic performance (V50) tests were performed to characterize the mechanical properties of the CNT composites. The CNT composites showed a reduction in interlaminar shear strength by 25.9%. They also showed an increase in the specific energy absorption by 106% at high strain rates and an increase in energy density dissipation by 643% after 5 cycles at quasi static strain rates. In ballistic V50 tests, the CNT based composites showed a higher V50 value by 11.1%. Due to their reduced weight and energy dissipation properties, the direct growth of CNTs on E-glass fabrics incorporated into composites have potential defense applications such as blast protection. Published by Elsevier Ltd. C1 [Boddu, Veera M.; Brenner, Matthew W.; Patel, Jignesh S.; Kumar, Ashok] US Army, ERDC, CERL, Champaign, IL 61821 USA. [Mantena, P. Raju; Tadepalli, Tezeswi; Pramanik, Brahmananda] Univ Mississippi, Dept Mech Engn, Composite Struct & Nanoengn Res, University, MS 38677 USA. [Tadepalli, Tezeswi] NIT, Dept Civil Engn, Warangal 5060004, TS, India. [Pramanik, Brahmananda] Univ Montana, Montana Tech, Dept Gen Engn, Butte, MT 59701 USA. RP Boddu, VM (reprint author), US Army, ERDC, CERL, Champaign, IL 61821 USA.; Boddu, VM (reprint author), ARS, Plant Polymer Res Unit, Natl Ctr Agr Res Ctr, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM veera.boddu@ars.usda.gov NR 56 TC 1 Z9 1 U1 10 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1359-8368 EI 1879-1069 J9 COMPOS PART B-ENG JI Compos. Pt. B-Eng. PD MAR 1 PY 2016 VL 88 BP 44 EP 54 DI 10.1016/j.compositesb.2015.10.028 PG 11 WC Engineering, Multidisciplinary; Materials Science, Composites SC Engineering; Materials Science GA DE8NT UT WOS:000370893500006 ER PT J AU Mondal, S Wenninger, EJ Hutchinson, PJS Whitworth, JL Shrestha, D Eigenbrode, SD Bosque-Perez, NA AF Mondal, Shaonpius Wenninger, Erik J. Hutchinson, Pamela J. S. Whitworth, Jonathan L. Shrestha, Deepak Eigenbrode, Sanford D. Bosque-Perez, Nilsa A. TI Comparison of transmission efficiency of various isolates of Potato virus Y among three aphid vectors SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE potato; green peach aphid; potato aphid; bird cherry-oat aphid; infection; virus titer; Myzus persicae; Macrosiphum euphorbiae; Rhopalosiphum padi; Hemiptera; Aphididae ID NECROTIC RINGSPOT DISEASE; GREEN PEACH APHID; MYZUS-PERSICAE; CEREAL APHIDS; UNITED-STATES; RT-PCR; STRAINS; PVYN; POTYVIRUS; PLANT AB Potato virus Y (PVY) strains are transmitted by different aphid species in a non-persistent, non-circulative manner. Green peach aphid (GPA), Myzus persicae Sulzer, is the most efficient vector in laboratory studies, but potato aphid (PA), Macrosiphum euphorbiae Thomas (both Hemiptera: Aphididae, Macrosiphini), and bird cherry-oat aphid (BCOA), Rhopalosiphum padi L. (Hemiptera: Aphididae, Aphidini), also contribute to PVY transmission. Studies were conducted with GPA, PA, and BCOA to assess PVY transmission efficiency for various isolates of the same strain. Treatments included three PVY strains (PVYO, PVYN:O, PVYNTN) and two isolates of each strain (Oz and NY090031 for PVYO; Alt and NY090004 for PVYN:O; N4 and NY090029 for PVYNTN), using each of three aphid species as well as a sham inoculation. Virus-free tissue-cultured plantlets of potato cv. Russet Burbank were used as virus source and recipient plants. Five weeks post inoculation, recipient plants were tested with quantitative DAS-ELISA to assess infection percentage and virus titer. ELISA-positive recipient plants were assayed with RT-PCR to confirm presence of the expected strains. Transmission efficiency (percentage infection of plants) was highest for GPA, intermediate for BCOA, and lowest for PA. For all aphid species, transmission efficiency did not differ significantly between isolates within each strain. No correlations were found among source plant titer, infection percentage, and recipient plant titer. For both GPA and BCOA, isolates of PVYNTN were transmitted with greatest efficiency followed by isolates of PVYO and PVYN:O, which might help explain the increasing prevalence of necrotic strains in potato-growing regions. Bird cherry-oat aphid transmitted PVY with higher efficiency than previously reported, suggesting that this species is more important to PVY epidemiology than has been considered. C1 [Mondal, Shaonpius; Hutchinson, Pamela J. S.; Shrestha, Deepak] Univ Idaho, Aberdeen Res & Extens Ctr, Dept Plant Soil & Entomol Sci, Aberdeen, ID 83210 USA. [Wenninger, Erik J.] Univ Idaho, Kimberly Res & Extens Ctr, Dept Plant Soil & Entomol Sci, Kimberly, ID 83341 USA. [Eigenbrode, Sanford D.; Bosque-Perez, Nilsa A.] Univ Idaho, Dept Plant Soil & Entomol Sci, 875 Perimeter Dr, Moscow, ID 83844 USA. [Whitworth, Jonathan L.] USDA ARS, Small Grains & Potato Germplasm Res, Aberdeen, ID 83210 USA. [Mondal, Shaonpius] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Sch Integrat Plant Sci, 334 Plant Sci Bldg, Ithaca, NY 14853 USA. [Shrestha, Deepak] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP Wenninger, EJ (reprint author), Univ Idaho, Kimberly Res & Extens Ctr, Dept Plant Soil & Entomol Sci, Kimberly, ID 83341 USA. EM erikw@uidaho.edu FU USDA-SCRI [2009-51181-05894]; Idaho Potato Commission FX We thank Stewart M. Gray, Alex Karasev, and Hanu R. Pappu for providing virus isolates and for their valuable suggestions for this study. We also thank William Price for advice on statistical analysis. We acknowledge the excellent technical assistance of Aretta Workman. Funding for this study was provided by USDA-SCRI (2009-51181-05894) and by the Idaho Potato Commission. This is a publication of the Idaho Agricultural Experiment Station. NR 63 TC 2 Z9 2 U1 14 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAR PY 2016 VL 158 IS 3 BP 258 EP 268 DI 10.1111/eea.12404 PG 11 WC Entomology SC Entomology GA DE9JG UT WOS:000370952200004 ER PT J AU Webber, H Ewert, F Kimball, BA Siebert, S White, JW Wall, GW Ottman, MJ Trawally, DNA Gaiser, T AF Webber, H. Ewert, F. Kimball, B. A. Siebert, S. White, J. W. Wall, G. W. Ottman, M. J. Trawally, D. N. A. Gaiser, T. TI Simulating canopy temperature for modelling heat stress in cereals SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Canopy temperature; Heat stress; Cereals; Crop models ID PROFILE RELATIONSHIPS; CROP PRODUCTION; CLIMATE-CHANGE; SPRING WHEAT; FIELD PLOTS; GROWTH; MAIZE; WATER; YIELDS; VARIABILITY AB Crop models must be improved to account for the effects of heat stress events on crop yields. To date, most approaches in crop models use air temperature to define heat stress intensity as the cumulative sum of thermal times (TT) above a high temperature threshold during a sensitive period for yield formation. However, observational evidence indicates that crop canopy temperature better explains yield reductions associated with high temperature events than air temperature does. This study presents a canopy level energy balance using Monin ObukhovSimilarity Theory (MOST) with simplifications about the canopy resistance that render it suitable for application in crop models and other models of the plant environment. The model is evaluated for a uniform irrigated wheat canopy in Arizona and rainfed maize in Burkina Faso. No single variable regression relationships for key explanatory variables were found that were consistent across sowing dates to explain the deviation of canopy temperature from air temperature. Finally, thermal times determined with simulated canopy temperatures were able to reproduce thermal times calculated with observed canopy temperature, whereas those determined with air temperatures were not. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Webber, H.; Ewert, F.; Siebert, S.; Gaiser, T.] Univ Bonn, Inst Crop Sci & Resource Conservat INRES, Crop Sci Grp, Katzenburgweg 5, D-53115 Bonn, Germany. [Kimball, B. A.; White, J. W.; Wall, G. W.] ARS, US Arid Land Agr Res Ctr, USDA, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Ottman, M. J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Trawally, D. N. A.] Kwame Nkrumah Univ Sci & Technol, Coll Engn, Dept Civil Engn, Kumasi, Ghana. RP Webber, H (reprint author), Univ Bonn, Inst Crop Sci & Resource Conservat INRES, Crop Sci Grp, Katzenburgweg 5, D-53115 Bonn, Germany. EM hwebber@uni-bonn.de RI Siebert, Stefan/B-8621-2009 OI Siebert, Stefan/0000-0002-9998-0672 FU Agricultural Research Service; U.S. Department of Agriculture; University of Arizona; WASCAL (West African Science Service Center on Climate Change and Adapted Land Use); Federal Ministry of Education and Research (BMBF) through WASCAL (West African Science ServiceCenter on Climate Change and Adapted Land Use) [01LG1202A]; German Science Foundation [EW 119/5-1]; FACCE JPI MACSUR project through the German Federal Ministry of Food and Agriculture [031A103B, 2812ERA115] FX Gunther Krauss and Andreas Enders are thanked for the help with the final implementation of the canopy temperature related modules. The technical assistance of Matt Conley in obtaining and analyzing the infrared thermometer data in the Hot Serial Cereal (HSC) experiment is greatly appreciated, as well as the assistance provided by Charles Blackshear, Mary Comeau, Steve Farnsworth, Justin Laughridge, Laura M. Olivieri and Dr. Zahra Troeh. The HSC experiment was supported by the Agricultural Research Service, U.S. Department of Agriculture and the University of Arizona. The experiment in Burkina Faso was supported by the research program and graduate research program of WASCAL (West African Science Service Center on Climate Change and Adapted Land Use). Abdoulaye Ouedraogo and Phillips Arnold of the Dreyer Foundation are thanked for facilitating the experiment, as are Dr. Isaac Danso, Idoure Dabirre and Dr. Sie Sylvestre Da for their assistance. HW, FE, TG and DT's contributions were funded by the Federal Ministry of Education and Research (BMBF, grant number 01LG1202A) through WASCAL (West African Science ServiceCenter on Climate Change and Adapted Land Use). SS and FE contributions were funded by the German Science Foundation (project EW 119/5-1). FE also acknowledges support from the FACCE JPI MACSUR project (031A103B) through the German Federal Ministry of Food and Agriculture (2812ERA115). NR 64 TC 6 Z9 6 U1 13 U2 41 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD MAR PY 2016 VL 77 BP 143 EP 155 DI 10.1016/j.envsoft.2015.12.003 PG 13 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DE8KN UT WOS:000370885000012 ER PT J AU Harmel, D Wagner, K Martin, E Smith, D Wanjugi, P Gentry, T Gregory, L Hendon, T AF Harmel, Daren Wagner, Kevin Martin, Emily Smith, Doug Wanjugi, Pauline Gentry, Terry Gregory, Lucas Hendon, Tina TI Effects of field storage method on E. coli concentrations measured in storm water runoff SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Fecal indicator bacteria; Water quality; Nonpoint source pollution ID ESCHERICHIA-COLI; QUALITY DATA; LAND-USE; TEXAS; TEMPERATURE; STREAMFLOW AB Storm water runoff is increasingly assessed for fecal indicator organisms (e.g., Escherichia coli, E. coli) and its impact on contact recreation. Concurrently, use of autosamplers along with logistic, economic, technical, and personnel barriers is challenging conventional protocols for sample holding times and storage conditions in the field. A common holding time limit for E. coli is 8 h with a 10 degrees C storage temperature, but several research studies support longer hold time thresholds. The use of autosamplers to collect E. coli water samples has received little field research attention; thus, this study was implemented to compare refrigerated and unrefrigerated autosamplers and evaluate potential E. coli concentration differences due to field storage temperature (storms with holding times <= 24 h) and due to field storage time and temperature (storms >24 h). Data from 85 runoff events on four diverse watersheds showed that field storage times and temperatures had minor effects on mean and median E. coli concentrations. Graphs and error values did, however, indicate a weak tendency for higher concentrations in the refrigerated samplers, but it is unknown to what extent differing die-off and/or regrowth rates, heterogeneity in concentrations within samples, and laboratory analysis uncertainty contributed to the results. The minimal differences in measured E. coli concentrations cast doubt on the need for utilizing the rigid conventional protocols for field holding time and storage temperature. This is not to say that proper quality assurance and quality control is not important but to emphasize the need to consider the balance between data quality and practical constraints related to logistics, funding, travel time, and autosampler use in storm water studies. C1 [Harmel, Daren; Smith, Doug] USDA ARS, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. [Wagner, Kevin; Gregory, Lucas] Texas A&M AgriLife Res, Texas Water Resources Inst, College Stn, TX USA. [Martin, Emily; Wanjugi, Pauline; Gentry, Terry] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Hendon, Tina] Tarrant Reg Water Dist, Ft Worth, TX USA. RP Harmel, D (reprint author), USDA ARS, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. EM daren.harmel@ars.usda.gov FU USDA-ARS; USEPA Region 6; Texas State Soil and Water Conservation Board FX Funding for this project was provided by USDA-ARS, USEPA Region 6, and the Texas State Soil and Water Conservation Board. USDA-ARS technicians at Riesel (Kyle Tiner, Gary Hoeft, and Larry Koester) and Texas A&M laboratory technicians (Heidi Mjelde) deserve credit for their outstanding technical support. NR 33 TC 0 Z9 0 U1 3 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD MAR PY 2016 VL 188 IS 3 AR 170 DI 10.1007/s10661-016-5183-9 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DF0ZG UT WOS:000371067600022 PM 26884357 ER PT J AU Livingston, DP Tuong, TD Isleib, TG Murphy, JP AF Livingston, David P., III Tuong, Tan D. Isleib, Thomas G. Murphy, J. Paul TI Differences between wheat genotypes in damage from freezing temperatures during reproductive growth SO EUROPEAN JOURNAL OF AGRONOMY LA English DT Article DE Wheat; Spring freeze; Heading; Infra red thermography; Supercooling; Barrier ID ICE PROPAGATION; WINTER-WHEAT; TOLERANCE; VERNALIZATION; PHOTOPERIOD; EMERGENCE; TISSUES; CEREALS; INJURY; PLANTS AB Cereal crops in the reproductive stage of growth are considerably more susceptible to injury from freezing temperatures than during their vegetative growth stage in the fall. While damage resulting from spring freeze events has been documented, information on genotypic differences intolerance to spring-freezes is scarce. Ninety wheat genotypes were subjected to a simulated spring-freeze at the mid-boot growth stage under controlled conditions. Spring-freeze tolerance was evaluated as the number of seeds per head at maturity after plants were frozen at 6 degrees C. Plants that froze, as confirmed by infrared (IR) thermography, died shortly after thawing and consequently the heads did not mature. Only in plants that had no visible freezing (super-cooled) were heads able to reach maturity and produce seeds. In plants that super-cooled four genotypes had significantly higher seed counts after being exposed to freezing than three with the lowest. In addition, significant differences between genotypes were found in whole plant survival among those that had frozen. Genotypes with high whole-plant freezing survival were not necessarily the same as the super-cooled plants with the highest seed counts. Spring-freeze tolerance was not correlated with maturity suggesting that improvement in freezing tolerance could be selected for without affecting heading date. Spring-freeze tolerance was not correlated with freezing tolerance of genotypes of plants in a vegetative state, either under non-acclimated or cold-acclimated conditions indicating that vegetative freezing tolerance is not a good predictor of spring-freeze tolerance. Published by Elsevier B.V. C1 [Livingston, David P., III; Tuong, Tan D.] ARS, USDA, 840 Method Rd,Unit 3, Raleigh, NC USA. [Livingston, David P., III; Isleib, Thomas G.; Murphy, J. Paul] N Carolina State Univ, 840 Method Rd,Unit 3, Raleigh, NC 27695 USA. RP Livingston, DP (reprint author), ARS, USDA, 840 Method Rd,Unit 3, Raleigh, NC USA. EM dpl@ncsu.edu FU North Carolina Small Grain Growers Association FX This research was conducted with USDA in-house funds and funding from the North Carolina Small Grain Growers Association. NR 31 TC 0 Z9 0 U1 5 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1161-0301 EI 1873-7331 J9 EUR J AGRON JI Eur. J. Agron. PD MAR PY 2016 VL 74 BP 164 EP 172 DI 10.1016/j.eja.2015.12.002 PG 9 WC Agronomy SC Agriculture GA DE8UR UT WOS:000370912700017 ER PT J AU Xie, JL Hse, CY Shupe, TF Hu, TX AF Xie, Jiulong Hse, Chung-Yun Shupe, Todd F. Hu, Tingxing TI Influence of solvent type on microwave-assisted liquefaction of bamboo SO EUROPEAN JOURNAL OF WOOD AND WOOD PRODUCTS LA English DT Article ID SUPERCRITICAL WATER; SPECTROSCOPY; PRODUCTS; RESIDUES; GLYCOL AB Microwave-assisted liquefaction of bamboo in glycerol, polyethylene glycerol (PEG), methanol, ethanol, and water were comparatively investigated by evaluating the temperature-dependence for conversion and liquefied residue characteristics. The conversion for the liquefaction in methanol, ethanol, and water increased with an increase in reaction temperature, while that for liquefaction in glycerol and PEG was converse. The results of Fourier transform-infrared spectra for the liquefied residues revealed that cellulose was the main resistance to bamboo liquefaction in methanol, ethanol, and water. Glycerol could be selected as a commendable liquefacient for the solvolysis of bamboo components at low temperature using microwave energy. Moreover, liquefaction behaviors in glycerol and methanol under different temperatures were also distinguished by scanning electron microscopy images. C1 [Xie, Jiulong; Hu, Tingxing] Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. [Hse, Chung-Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Xie, Jiulong; Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. RP Hu, TX (reprint author), Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. EM tingxing_hu@163.com NR 18 TC 2 Z9 2 U1 4 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0018-3768 EI 1436-736X J9 EUR J WOOD WOOD PROD JI Eur. J. Wood Wood Prod. PD MAR PY 2016 VL 74 IS 2 BP 249 EP 254 DI 10.1007/s00107-016-1009-2 PG 6 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA DE7KQ UT WOS:000370815700015 ER PT J AU Truchan, HK Cockburn, CL Hebert, KS Magunda, F Noh, SM Carlyon, JA AF Truchan, Hilary K. Cockburn, Chelsea L. Hebert, Kathryn S. Magunda, Forgivemore Noh, Susan M. Carlyon, Jason A. TI The Pathogen-Occupied Vacuoles of Anaplasma phagocytophilum and Anaplasma marginale Interact with the Endoplasmic Reticulum SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE Anaplasmataceae; Rickettsia; intracellular bacteria; endoplasmic reticulum; Rab; pathogen synapse ID OBLIGATORY INTRACELLULAR INFECTION; CHLAMYDIA-TRACHOMATIS; LEGIONELLA-PNEUMOPHILA; ENDOTHELIAL-CELLS; PARASITOPHOROUS VACUOLE; INCLUSION MEMBRANE; RAB-GTPASES; PROTEIN; DEGRADATION; TRAFFICKING AB The genus Anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. A. phagocytophilum, a human and veterinary pathogen, infects neutrophils to cause granulocytic anaplasmosis. A. marginate invades bovine erythrocytes. Evidence suggests that both species may also infect endothelial cells in vivo. In mammalian and arthropod host cells, A. phagocytophilum and A. marginale reside in host cell derived pathogen-occupied vacuoles (POVs). While it was recently demonstrated that the A. phagocytophilum-occupied vacuole (ApV) intercepts membrane traffic from the trans-Golgi network, it is unclear if it or the A. marginale-occupied vacuole (AmV) interacts with other secretory organelles. Here, we demonstrate that the ApV and AmV extensively interact with the host endoplasmic reticulum (ER) in endothelial, myeloid, and/or tick cells. ER lumen markers, calreticulin, and protein disulfide isomerase, and the ER membrane marker, derlin-1, were pronouncedly recruited to the peripheries of both POVs. ApV association with the ER initiated early and continued throughout the infection cycle. Both the ApV and AmV interacted with the rough ER and smooth ER. However, only derlin-1-positive rough ER derived vesicles were delivered into the ApV lumen where they localized with intravacuolar bacteria. Transmission electron microscopy identified multiple ER-POV membrane contact sites on the cytosolic faces of both species' vacuoles that corresponded to areas on the vacuoles' lumenal faces where intravacuolar Anaplasma organisms closely associated. A. phagocytophilum is known to hijack Rab10, a GTPase that regulates ER dynamics and morphology. Yet, ApV-ER interactions were unhindered in cells in which Rab10 had been knocked down, demonstrating that the GTPase is dispensable for the bacterium to parasitize the ER. These data establish the ApV and AmV as pathogen-host interfaces that directly engage the ER in vertebrate and invertebrate host cells and evidence the conservation of ER parasitism between two Anaplasma species. C1 [Truchan, Hilary K.; Cockburn, Chelsea L.; Hebert, Kathryn S.; Carlyon, Jason A.] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Sch Med, Richmond, VA 23298 USA. [Magunda, Forgivemore; Noh, Susan M.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Magunda, Forgivemore] Washington State Univ, Paul G Allen Sch Global Anima Hlth, Pullman, WA 99164 USA. [Noh, Susan M.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Truchan, Hilary K.] Northwestern Univ, Sch Med, Dept Microbiol & Immunol, Chicago, IL 60611 USA. RP Carlyon, JA (reprint author), Virginia Commonwealth Univ, Dept Microbiol & Immunol, Sch Med, Richmond, VA 23298 USA. EM jason.carlyon@vcuhealth.org OI Magunda, Forgivemore/0000-0001-7854-5818 FU National Institutes of Health [R01 AI072683, R37 A144005]; National Center for Advancing Translational Sciences Grant [UL1TR000058]; Center for Clinical and Translational Research Endowment Fund of VCU; NIH-NINDS Center core grant [5P30NS047463]; NIH-NCI Cancer Center Support Grant [P30 CA016059]; U. S. Department of Agriculture-Agricultural Research Service Project [5348-32000-033-00D] FX This study was supported by funding from National Institutes of Health Grants R01 AI072683, National Center for Advancing Translational Sciences Grant UL1TR000058, and the Center for Clinical and Translational Research Endowment Fund of VCU (to JC). LSCM, SIM, and electron microscopy were performed at the VCU Microscopy Facility, which is supported in part with funding from NIH-NINDS Center core grant 5P30NS047463 and NIH-NCI Cancer Center Support Grant (P30 CA016059). SN: U. S. Department of Agriculture-Agricultural Research Service Project #5348-32000-033-00D and National Institutes of Health R37 A144005. NR 68 TC 4 Z9 4 U1 2 U2 6 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD MAR 1 PY 2016 VL 6 AR 22 DI 10.3389/fcimb.2016.00022 PG 16 WC Immunology; Microbiology SC Immunology; Microbiology GA DE9TW UT WOS:000370983300001 PM 26973816 ER PT J AU Leone, A Chang, S AF Leone, Angela Chang, Sarah TI A Winning Combination: Introducing the MyPlate, MyWins Campaign and MyPlate Challenges SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Leone, Angela; Chang, Sarah] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. RP Leone, A (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAR PY 2016 VL 116 IS 3 BP 401 EP 404 DI 10.1016/j.jand.2015.12.024 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DE6DF UT WOS:000370723100005 PM 26920237 ER PT J AU Tooley, PW Browning, M Shishkoff, N AF Tooley, Paul W. Browning, Marsha Shishkoff, Nina TI Pyracantha 'Mohave' Fruit Infection by Phytophthora ramorum and Transmission of the Pathogen from Infected Fruit to Roots of Viburnum tinus SO PLANT DISEASE LA English DT Article ID SUDDEN OAK DEATH; PLANT PATHOLOGY; INOCULUM; CALIFORNIA; FOREST; ROT; CHLAMYDOSPORES; SUSCEPTIBILITY; RHODODENDRON; TEMPERATURE AB Colonization of the fleshy fruit of Cornus florida, C. kousa, Laurus nobilis, Mains hupehensis, and Pyracantha 'Mohave' was observed following inoculation with sporangia of Phytophthora ramorum. However, abundant production of chlamydospores was only observed in the fruit of Pyracantha 'Mohave'. Pyracantha 'Mohave' fruit that had been inoculated with a P. ramorum sporangia suspension were placed in pots containing rooted cuttings of Viburnum tinus in a misting tent or in water-filled trays in a climate-controlled greenhouse. Runoff was collected for 24 to 30 days, and roots were plated after the final collection. Mean percent recovery from infected roots was not significantly different (P = 0.05, Tukey' s test) between bottom watered treatments in trays and misted treatments, averaging 58% for bottom-watered and 54% for mist treatments. The number of CFU collected in runoff from bottom-watered plants was consistently lower than that obtained from plants held under mist, likely due to desiccation of the fruit. The results show that root infection of V. tinus can occur by P. ramorum via infected fruit of Pyracantha 'Mohave'. This phenomenon represents a pathway of infection for P. ramorum not previously reported, which may play a role in disease epidemiology. C1 [Tooley, Paul W.; Browning, Marsha; Shishkoff, Nina] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. RP Tooley, PW (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. EM paul.tooley@ars.usda.gov NR 46 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2016 VL 100 IS 3 BP 555 EP 560 DI 10.1094/PDIS-03-15-0369-RE PG 6 WC Plant Sciences SC Plant Sciences GA DE9YP UT WOS:000370996100002 ER PT J AU Li, R Berendsen, S Ling, KS AF Li, Rugang Berendsen, Sven Ling, Kai-Shu TI A Duplex Real-Time RT-PCR System with an Internal Control Offers Sensitive and Reliable Broad-Spectrum Detection of Squash mosaic virus Variants SO PLANT DISEASE LA English DT Article ID COAT PROTEIN GENES; TRANSMISSION; SEQUENCE; IDENTIFICATION; VARIABILITY; CALIFORNIA; STRAINS; SEED AB Squash mosaic virus (SqMV), a seedbome virus, belongs to the genus Comovirus in the subfamily Comovirinae of the family Secoviridae. SqMV has a bipartite single-stranded RNA genome (RNA1 and RNA2) encapsidated separately with two capsid proteins. With the recent identification of a third genotype in SqMV, a greater genetic diversity with only 88 to 89% sequence identity among them are recognized. With the existence of genetic diversity, a previously developed quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) failed to detect isolates in this new genotype. Therefore, it was necessary to create a new qRT-PCR that would react with all SqMV isolates in three different genotypes. From a multiple sequence alignment of the available SqMV sequences in GenBank, a conserved sequence segment in the 3' untranslated region of RNA2 was identified for primer and probe design. A new qRT-PCR was developed, which provided broad-spectrum reactions to SqMV isolates, including those from the newly recognized third genotype. To improve the reliability in determining the sample quality and result interpretation, an internal amplification control with an endogenous gene sequence (18S ribosomal RNA) was successfully incorporated to develop a duplex qRT-PCR system that was useful for seed health test. C1 [Li, Rugang; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC USA. [Berendsen, Sven] Rijk Zwaan Breeding BV De Lier, De Lier, Netherlands. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, Charleston, SC USA. EM Kai.Ling@ARS.USDA.GOV FU USDA NIFA-SCRI [SCRI 2010-600-25320, 2012-01507-229756] FX We thank A. Gilliard for technical assistance. This work was supported, in part, by USDA NIFA-SCRI (SCRI 2010-600-25320 and 2012-01507-229756) to K.-S. Ling. NR 22 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2016 VL 100 IS 3 BP 625 EP 629 DI 10.1094/PDIS-08-15-0944-RE PG 5 WC Plant Sciences SC Plant Sciences GA DE9YP UT WOS:000370996100011 ER PT J AU Okubara, PA Leston, N Micknass, U Kogel, KH Imani, J AF Okubara, Patricia A. Leston, Natalie Micknass, Ute Kogel, Karl-Heinz Imani, Jafargholi TI Rapid Quantitative Assessment of Rhizoctonia Resistance in Roots of Selected Wheat and Barley Genotypes SO PLANT DISEASE LA English DT Article ID SOLANI AG-8; PACIFIC-NORTHWEST; REDUCED TILLAGE; HEXAPLOID WHEAT; BARE PATCH; ROT; PATHOGENS; CEREALS; TRAITS; GRAINS AB Rhizoctonia solani AG8, causal agent of Rhizoctonia root rot and bare patch in dryland cereal production systems of the Pacific Northwest United States and Australia, reduces yields in a wide range of crops. Disease is not consistently controlled by available management practices, so genetic resistance would be a desirable resource for growers. In this report, we describe three rapid and low-cost assays for R. solani AG8 resistance in wheat and barley, with the view of facilitating screens for genetic resistance in these hosts. The first assay uses 50-m1 conical centrifuge tubes containing soil infested with R. solani AG8 on a substrate of ground oats. The second assay uses roots of 3-day-old seedlings directly coated with infested ground oats, followed by incubation in plastic dishes. The third assay, suitable for barley, uses whole infested oat kernels in 50-m1 tubes. Symptoms are quantified on the bases of root fresh weight and total root length at 7 and 3 days for the tube and coating assays, respectively. Each of the assays show the same disease differential between susceptible and partially resistant wheat genotypes. The assays can be conducted in the laboratory, growth chamber, or greenhouse. C1 [Okubara, Patricia A.] USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Leston, Natalie] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Micknass, Ute; Kogel, Karl-Heinz; Imani, Jafargholi] Univ Giessen, Inst Phytopathol, Res Ctr BioSyst Land Use & Nutr IFZ, D-35392 Giessen, Germany. RP Okubara, PA (reprint author), USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. EM patricia.okubara@ars.usda.gov FU German Academic Exchange Service (DAAD) [A/13/09014]; Washington Grain Commission [3061-4548]; USDA ARS Project [2090 22000 016 00D] FX This work was supported by German Academic Exchange Service (DAAD) grant A/13/09014, Washington Grain Commission grant 3061-4548, and USDA ARS Project Number 2090 22000 016 00D (P.O.). NR 22 TC 0 Z9 0 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2016 VL 100 IS 3 BP 640 EP 644 DI 10.1094/PDIS-05-15-0611-SR PG 5 WC Plant Sciences SC Plant Sciences GA DE9YP UT WOS:000370996100013 ER PT J AU Pereira, TBC Dally, EL Davis, RE Banzato, TC Bedendo, IP AF Pereira, T. B. C. Dally, E. L. Davis, R. E. Banzato, T. C. Bedendo, I. P. TI Ming Aralia (Polyscias fruticose), a New Host of a Phytoplasma Subgroup 16SrVII-B Strain in Brazil. SO PLANT DISEASE LA English DT News Item ID CLASSIFICATION; YELLOWS C1 [Pereira, T. B. C.; Banzato, T. C.; Bedendo, I. P.] ESALQ USP, Dept Plant Pathol & Nematol, BR-13418900 Sao Paulo, Brazil. [Dally, E. L.; Davis, R. E.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Pereira, TBC (reprint author), ESALQ USP, Dept Plant Pathol & Nematol, BR-13418900 Sao Paulo, Brazil. NR 4 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2016 VL 100 IS 3 BP 645 EP 645 DI 10.1094/PDIS-03-15-0254-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DE9YP UT WOS:000370996100014 ER PT J AU Yan, Z Rascoe, J Kumagai, LB Keremane, ML Nakhla, MK AF Yan, Z. Rascoe, J. Kumagai, L. B. Keremane, M. L. Nakhla, M. K. TI Citrus Huanglongbing (HLB) Discoveries in California in 2015 and 2012 are of Different Genotypes of Candidates Liberibacter asiaticus (cLas) by Double-locus Genomic Variation Analysis. SO PLANT DISEASE LA English DT News Item ID PCR C1 [Yan, Z.; Rascoe, J.; Nakhla, M. K.] USDA APHIS PPQ CPHST, Beltsville, MD 20705 USA. [Kumagai, L. B.] CDFA, Sacramento, CA 95832 USA. [Keremane, M. L.] USDA ARS, Riverside, CA 92507 USA. RP Yan, Z (reprint author), USDA APHIS PPQ CPHST, Beltsville, MD 20705 USA. NR 4 TC 0 Z9 0 U1 5 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2016 VL 100 IS 3 BP 645 EP 645 DI 10.1094/PDIS-09-15-1059-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DE9YP UT WOS:000370996100015 ER PT J AU Rivera, Y Salgado-Salazar, C Windham, AS Crouch, JA AF Rivera, Y. Salgado-Salazar, C. Windham, A. S. Crouch, J. A. TI Downy Mildew on Coleus (Plectranthus scutellarioides) Caused by Peronospora belbahrii sensu lato in Tennessee. SO PLANT DISEASE LA English DT News Item C1 [Rivera, Y.; Salgado-Salazar, C.; Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Rivera, Y.; Salgado-Salazar, C.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Windham, A. S.] Univ Tennessee, Soil Plant & Pest Ctr, Dept Entomol & Plant Pathol, Nashville, TN 37211 USA. RP Rivera, Y (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA.; Rivera, Y (reprint author), Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. NR 4 TC 1 Z9 1 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAR PY 2016 VL 100 IS 3 BP 655 EP 655 PG 1 WC Plant Sciences SC Plant Sciences GA DE9YP UT WOS:000370996100040 ER PT J AU Bodah, ET Porter, LD Chaves, B Dhingra, A AF Bodah, Eliane T. Porter, Lyndon D. Chaves, Bernardo Dhingra, Amit TI Evaluation of pea accessions and commercial cultivars for fusarium root rot resistance SO EUPHYTICA LA English DT Article DE Disease resistance; Fusarium solani f. sp pisi; Fusarium root rot; Pea; Pisum sativum ID PISUM-SATIVUM; GERMPLASM; PATHOGENICITY; AVENACEUM; SEQUENCE; SOILS; FOOT AB Fusarium root rot caused by Fusarium solani f. sp. pisi (Fsp) can result in major yield losses in pea (Pisum sativum L.). Currently no fungicides effectively manage this disease. Previous studies evaluated the Pisum germplasm collection for resistance to Fsp, however, evaluations of commercial market classes of pea cultivars grown in the US, and elsewhere, have not been reported. This study evaluated pea accessions and commercial cultivars for Fsp resistance under greenhouse conditions. Accessions evaluated included pigmented lines with high levels of partial resistance to Fsp. Based on root disease severity (RDS) values, the most Fsp-resistant Austrian winter, green fresh, green dry, yellow dry, green winter and yellow winter pea were: PI 125673, 5003, 'Banner', 'Carneval', PS 05300234, and 'Whistler', respectively. Genotypes with a RDS value of 3.05, on a 0-6 scale with 6 being severe, or less had no significant (P a parts per thousand currency sign 0.05) reductions in plant height, shoot dry weight, and root dry weight, compared to non-inoculated controls, establishing an important disease threshold value for pea breeders. Plant height, more than shoot dry weight or root dry weight, was the most highly negatively correlated growth parameter related to RDS in repeated tests based on Pearson's Correlation coefficients. However, root dry weight was also a highly sensitive growth parameter affected by Fsp since 14 of 33 genotypes had significant (P a parts per thousand currency sign 0.05) reductions in root dry weight compared to non-inoculated controls, while only 5 of 33 genotypes had significant reductions in plant height and/or shoot dry weight in combined tests. C1 [Bodah, Eliane T.; Dhingra, Amit] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Porter, Lyndon D.; Chaves, Bernardo] USDA ARS, Grain Legume Genet & Physiol Res Unit, Prosser, WA 99350 USA. RP Porter, LD (reprint author), USDA ARS, Grain Legume Genet & Physiol Res Unit, Prosser, WA 99350 USA. EM lyndon.porter@ars.usda.gov RI Dhingra, Amit/E-7369-2010 FU Washington State University Agricultural Research Center Hatch Funds; United States Department of Agriculture, Agricultural Research Service FX The authors would like to thank Virginia Coffman and Tyler McClure, United States Department of Agriculture, Agricultural Research Service, Prosser, WA for help with the greenhouse studies; Brian Bodah, Washington State University, Pierce County Extension for help with greenhouse studies and sampling; Adrian Russell, NZ Plant Research LTD and Bob Arthur, Crites Seed Incorporation for guidance on commercial varieties to be used in this study; Mike Wood, Kurt Braunwart and Chris Braunwart, ProGene Plant Research, for intellectual and financial support and sponsorship. The authors would also like to thank Washington State University Agricultural Research Center Hatch Funds to AD and the United States Department of Agriculture, Agricultural Research Service, for supporting this research. NR 34 TC 0 Z9 0 U1 5 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAR PY 2016 VL 208 IS 1 BP 63 EP 72 DI 10.1007/s10681-015-1545-6 PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DE0TK UT WOS:000370337500004 ER PT J AU Shi, AN Buckley, B Mou, BQ Motes, D Morris, JB Ma, JB Xiong, HZ Qin, J Yang, W Chitwood, J Weng, YJ Lu, WG AF Shi, Ainong Buckley, Blair Mou, Beiquan Motes, Dennis Morris, J. Bradley Ma, Jianbing Xiong, Haizheng Qin, Jun Yang, Wei Chitwood, Jessica Weng, Yuejin Lu, Weiguo TI Association analysis of cowpea bacterial blight resistance in USDA cowpea germplasm SO EUPHYTICA LA English DT Article DE Cowpea; Vigna unguiculata; Bacterial blight; Single nucleotide polymorphism (SNP); Genotyping by sequencing (GBS); Association analysis ID MARKER-ASSISTED SELECTION; SEQUENCING GBS; CROP IMPROVEMENT; GENETIC-MAP; LOCI; TOOL; IDENTIFICATION; VIGNICOLA; SOFTWARE; TRAITS AB Cowpea bacterial blight (CoBB, Xanthomonas axonopodis pv. vignicola, Xav) is the most important bacterial disease of cowpea (Vigna unguiculata) because it prevalent in all major cowpea growing areas worldwide, and the use of host resistance is the primary method to control this disease. Genetic diversity and association analysis were conducted for CoBB resistance in 249 USDA germplasm accessions, originally collected from 42 countries. Genotyping by sequencing (GBS) was used for single nucleotide polymorphism (SNP) discovery. A total of 1031 SNPs were used for genetic diversity and association analysis in this study. Three well-differentiated genetic populations and admixtures were postulated in the cowpea panel by STRUCTURE 2.3.4 and MEGA 6. Association analysis for CoBB resistance was done using single marker regression, general linear mode, and mixed linear mode using Tassel 5, GAPIT and QGene 4. Four SNP markers (C35046071_1260, C35084634_455, scaffold96328_3387, and scaffold96765_4430) were identified to be strongly associated with CoBB resistance with > 70 % selection accuracy. These markers can be utilized in cowpea breeding for CoBB resistance through marker-assisted selection. C1 [Shi, Ainong; Ma, Jianbing; Xiong, Haizheng; Qin, Jun; Yang, Wei; Chitwood, Jessica; Weng, Yuejin; Lu, Weiguo] Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA. [Buckley, Blair] LSU AgCtr, Red River Res Stn, 262 Res Stn Dr, Bossier City, LA 71112 USA. [Mou, Beiquan] USDA ARS, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. [Motes, Dennis] Univ Arkansas, Vegetable Res Ctr, Alma, AR 72921 USA. [Morris, J. Bradley] USDA, Plant Genet Resources Conservat Unit, 1109 Expt St, Griffin, GA 30223 USA. RP Shi, AN (reprint author), Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA.; Buckley, B (reprint author), LSU AgCtr, Red River Res Stn, 262 Res Stn Dr, Bossier City, LA 71112 USA.; Mou, BQ (reprint author), USDA ARS, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. EM ashi@uark.edu; bbuckley@agcenter.lsu.edu; beiquan.mou@ars.usda.gov FU USDA-ARS GRIN GERMPLASM EVALUATION PROPOSAL for National Plant Germplasm System (NPGS) by Crop Germplasm Committee (CGC) [6046-21000-011-15] FX This work was supported by USDA-ARS GRIN GERMPLASM EVALUATION PROPOSAL for National Plant Germplasm System (NPGS) by Crop Germplasm Committee (CGC) with Project Number: 6046-21000-011-15. Cowpea germplasm accessions were provide by USDA-ARS at GRIFFIN, GA Station. The cowpea Genome 0.03.fa (6750 scaffolds or contigs) (http://harvest-blast.org/) was kindly provided by Dr. Timothy J. Close at University of California Riverside, CA, USA. NR 49 TC 3 Z9 3 U1 1 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAR PY 2016 VL 208 IS 1 BP 143 EP 155 DI 10.1007/s10681-015-1610-1 PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DE0TK UT WOS:000370337500011 ER PT J AU Eizenga, GC Neves, PCF Bryant, RJ Agrama, HA Mackill, DJ AF Eizenga, Georgia C. Neves, Pericles C. F. Bryant, Rolfe J. Agrama, Hesham A. Mackill, David J. TI Evaluation of a M-202 x Oryza nivara advanced backcross mapping population for seedling vigor, yield components and quality SO EUPHYTICA LA English DT Article DE Advanced backcross method; QTL mapping population; Amylose; Alkali spreading value; Interspecific cross; Oryza sativa ID QUANTITATIVE TRAIT LOCI; SINGLE-NUCLEOTIDE POLYMORPHISMS; RECOMBINANT INBRED LINES; RELATIVE O-RUFIPOGON; STARCH-SYNTHASE-IIA; SATIVA VARIETY IR64; CULTIVATED RICE; GRAIN QUALITY; BLAST RESISTANCE; QTL ANALYSIS AB Oryza nivara, the ancestral species of cultivated rice (O. sativa), has been the source of novel alleles for resistance to biotic and abiotic stress lost during domestication. Interspecific advanced backcross (ABC) populations permit the introgression of desirable alleles from the wild species into O. sativa and allow traits to be mapped to chromosomal regions by QTL mapping. An ABC population was developed by crossing M-202, a California medium grain, temperate japonica cultivar with O. nivara (IRGC100195). The population has 177 BC2F2:5 progeny lines and was evaluated for 17 traits including seedling vigor under cool temperature (mesocotyl, coleoptile, shoot and root lengths), agronomic (days to heading, plant height, culm angle, panicle type), yield components (panicles per plant, panicle length, florets and seeds per panicle, 100-seed weight) and quality [kernel length and width, apparent amylose content (AAC), alkali spreading value (ASV)]. Most exciting was that the O. nivara parent improved seedling vigor by increasing both the coleoptile and shoot lengths. Wild donor alleles increased the panicles per plant and seed weight, but M-202 alleles improved fertility. For one locus, the O. nivara alleles accounted for increased kernel length even though this parent had smaller seeds than M-202. The AAC mapped to the WAXY locus and ASV to the ALK locus, with most progeny being similar to M-202 for these quality traits. Select progeny lines could be useful for improving seedling vigor. This interspecific population is the first in the background of a U.S. temperate japonica rice cultivar. C1 [Eizenga, Georgia C.; Bryant, Rolfe J.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Highway 130 East, Stuttgart, AR 72160 USA. [Neves, Pericles C. F.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Neves, Pericles C. F.] EMBRAPA Arroz & Feija, CP 179,75-375-000 Sto, Antonio De Goias, Go, Brazil. [Agrama, Hesham A.] Univ Arkansas, Rice Res & Extens Ctr, 2900 Highway 130 East, Stuttgart, AR 72150 USA. [Agrama, Hesham A.] Sultan Qaboos Univ, Coll Agricultual & Marine Sci, 34,Al Khoud 123, Muscat, Oman. [Mackill, David J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Mackill, David J.] Univ Calif Davis, Mars Inc, Dept Plant Sci, Davis, CA 95616 USA. RP Eizenga, GC (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Highway 130 East, Stuttgart, AR 72160 USA. EM georgia.eizenga@ARS.USDA.GOV FU Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA); University of California; USDA-ARS at Davis, California; Arkansas Rice Research and Promotion Board through the University of Arkansas FX The financial support of the Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA) to P.C.F. Neves is gratefully acknowledged, as well as the support of the University of California and USDA-ARS at Davis, California, for the research conducted at Davis. The studies conducted at Davis, California, were part of the Ph.D. dissertation research for P.C.F. Neves. Dr. Thomas H. Tai is acknowledged for sharing the seed with G.C. Eizenga. At the USDA-ARS DB NRRC in Stuttgart, Arkansas, the excellent technical assistance of Quynh P.-H. Grunden is acknowledged in all phases of the work. Both Melissa H. Jia and Aaron K. Jackson in the Genomics Core Facility are recognized for running markers, allele calling and assisting with creating the final linkage map. The support of the Arkansas Rice Research and Promotion Board through the University of Arkansas for H.A. Agrama is gratefully acknowledged. Lastly, the excellent review with suggestions for improving the manuscript by Dr. Shannon R. Pinson is gratefully appreciated. NR 70 TC 0 Z9 0 U1 5 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAR PY 2016 VL 208 IS 1 BP 157 EP 171 DI 10.1007/s10681-015-1613-y PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DE0TK UT WOS:000370337500012 ER PT J AU Lyu, L Whitcomb, EA Jiang, SH Chang, ML Gu, YM Duncan, MK Cvekl, A Wang, WL Limi, S Reneker, LW Shang, F Du, LF Taylor, A AF Lyu, Lei Whitcomb, Elizabeth A. Jiang, Shuhong Chang, Min-Lee Gu, Yumei Duncan, Melinda K. Cvekl, Ales Wang, Wei-Lin Limi, Saima Reneker, Lixing W. Shang, Fu Du, Linfang Taylor, Allen TI Unfolded-protein response-associated stabilization of p27(Cdkn1b) interferes with lens fiber cell denucleation, leading to cataract SO FASEB JOURNAL LA English DT Article DE ubiquitin; cell cycle; endoplasmic reticulum stress; nuclear disassembly ID DEPENDENT KINASE INHIBITOR; MOUSE EYE LENS; A-TYPE LAMINS; ENDOPLASMIC-RETICULUM; ER STRESS; PHOSPHORYLATION SITES; UBIQUITIN LIGASE; CDK INHIBITORS; NUCLEAR LAMINA; P27(KIP1) AB Failure of lens fiber cell denucleation (LFCD) is associated with congenital cataracts, but the pathobiology awaits elucidation. Recent work has suggested that mechanisms that direct the unidirectional process of LFCD are analogous to the cyclic processes associated with mitosis. We found that lens-specific mutations that elicit an unfolded-protein response (UPR) in vivo accumulate p27(Cdkn1b), show cyclin-dependent kinase (Cdk)-1 inhibition, retain their LFC nuclei, and are cataractous. Although a UPR was not detected in lenses expressing K6W-Ub, they also accumulated p27 and showed failed LFCD. Induction of a UPR in human lens epithelial cells (HLECs) also induced accumulation of p27 associated with decreased levels of S-phase kinase-associated protein (Skp)-2, a ubiquitin ligase that regulates mitosis. These cells also showed decreased lamin A/C phosphorylation and metaphase arrest. The suppression of lamin A/C phosphorylation and metaphase transition induced by the UPR was rescued by knockdown of p27. Taken together, these data indicate that accumulation of p27, whether related to the UPR or not, prevents the phosphorylation of lamin A/C and LFCD in maturing LFCs in vivo, as well as in dividing HLECs. The former leads to cataract and the latter to metaphase arrest. These results suggest that accumulation of p27 is a common mechanism underlying retention of LFC nuclei. C1 [Lyu, Lei; Du, Linfang] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresource & Ecoenvironm, Chengdu 610064, Sichuan, Peoples R China. [Lyu, Lei; Whitcomb, Elizabeth A.; Jiang, Shuhong; Chang, Min-Lee; Gu, Yumei; Shang, Fu; Taylor, Allen] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. [Duncan, Melinda K.] Univ Delaware, Dept Biol Sci, Newark, DE USA. [Cvekl, Ales; Wang, Wei-Lin; Limi, Saima] Albert Einstein Coll Med, Dept Genet, Bronx, NY 10467 USA. [Cvekl, Ales] Albert Einstein Coll Med, Dept Ophthalmol & Visual Sci, Bronx, NY 10467 USA. [Reneker, Lixing W.] Univ Missouri, Sch Med, Dept Ophthalmol, Columbia, MO USA. RP Du, LF (reprint author), Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresource & Ecoenvironm, Chengdu 610064, Sichuan, Peoples R China.; Taylor, A (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM dulinfang@scu.edu.cn; allen.taylor@tufts.edu FU U.S. Department of Agriculture, Agricultural Research Service [58-1950-4-003]; U.S. National Institutes of Health, National Eye Institute [EY13250, EY11717]; China Scholarship Council FX The authors thank Dr. S. Nagata for providing the DNAse II beta antibody; Dr. P. Gonczy for the gift of the pNUMA antibody; and Dr. S. Rowan, L. Bailey, and Z. Crosser for critical reading and suggestions during the preparation of the manuscript. This work was supported by U.S. Department of Agriculture, Agricultural Research Service Agreement 58-1950-4-003; U.S. National Institutes of Health, National Eye Institute Grants EY13250 and EY11717; and the China Scholarship Council. NR 59 TC 2 Z9 2 U1 2 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD MAR PY 2016 VL 30 IS 3 BP 1087 EP 1095 DI 10.1096/fj.15-278036 PG 9 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA DE1LF UT WOS:000370387800008 PM 26590164 ER PT J AU Glowacka, K Ahmed, A Sharma, S Abbott, T Comstock, JC Long, SP Sacks, EJ AF Glowacka, Katarzyna Ahmed, Aasifuddin Sharma, Shailendra Abbott, Tom Comstock, Jack C. Long, Stephen P. Sacks, Erik J. TI Can chilling tolerance of C-4 photosynthesis in Miscanthus be transferred to sugarcane? SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE bioenergy crop; chilling; cold tolerance; miscane; Miscanthus xgiganteus; photosynthesis; plant breeding; Saccharum officinarum; sugarcane ID SACCHARUM-SPONTANEUM; TEMPERATE CLIMATES; GENOTYPES; AFLP; REGISTRATION; MARKERS; YIELD; SPP.; GERMPLASM; GRASSES AB The goal of this study was to investigate whether chilling tolerance of C-4 photosynthesis in Miscanthus can be transferred to sugarcane by hybridization. Net leaf CO2 uptake (A(sat)) and the maximum operating efficiency of photosystem II (?(PSII)) were measured in warm conditions (25 degrees C/20 degrees C), and then during and following a chilling treatment of 10 degrees C/5 degrees C for 11day in controlled environment chambers. Two of three hybrids (miscanes), US 84-1058' and US 87-1019', did not differ significantly from the chilling tolerant M. xgiganteus Illinois' (Mxg), for A(sat), and phi(PSII) measured during chilling. For Mxg grown at 10 degrees C/5 degrees C for 11days, A(sat) was 4.4mol m(-2) s(-1), while for miscane US 84-1058' and US 87-1019', A(sat) was 5.7 and 3.5mol m(-2) s(-1), respectively. Miscanes US 84-1058' and US 87-1019' and Mxg had significantly higher rates of A(sat) during chilling than three tested sugarcanes. A third miscane showed lower rates than Mxg during chilling, but recovered to higher rates than sugarcane upon return to warm conditions. Chilling tolerance of US 84-1058' was further confirmed under autumn field conditions in southern Illinois. The selected chilling tolerant miscanes have particular value for biomass feedstock and biofuel production and at the same time they can be a starting point for extending sugarcane's range to colder climates. C1 [Glowacka, Katarzyna; Ahmed, Aasifuddin; Sharma, Shailendra; Long, Stephen P.; Sacks, Erik J.] Univ Illinois, Inst Genom Biol, 1206 W Gregory Dr, Urbana, IL 61801 USA. [Glowacka, Katarzyna] Polish Acad Sci, Inst Plant Genet, Ul Strzeszynska 34, PL-60479 Poznan, Poland. [Abbott, Tom; Comstock, Jack C.] ARS, USDA, Sugarcane Field Stn, 12990 US HWY 441 N, Canal Point, FL 33438 USA. RP Glowacka, K (reprint author), Univ Illinois, Inst Genom Biol, 1206 W Gregory Dr, Urbana, IL 61801 USA.; Glowacka, K (reprint author), Polish Acad Sci, Inst Plant Genet, Ul Strzeszynska 34, PL-60479 Poznan, Poland. EM uszepti@tlen.pl FU Advanced Research Projects Agency-Energy (ARPA-E), USA, Department of Energy [DE-AR0000206] FX We thank Andrew Hauck and Juan Marroquin for help with plant material propagation; Steven Moose for providing the maize FR1064 seeds from Illinois Foundation Seeds (Tolono, IL, USA); and members of the Long Laboratory for stimulating discussions during experiment design and writing the manuscript. The data presented herein were funded in part by the Advanced Research Projects Agency-Energy (ARPA-E), USA, Department of Energy, under Award Number DE-AR0000206. NR 57 TC 2 Z9 2 U1 3 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD MAR PY 2016 VL 8 IS 2 BP 407 EP 418 DI 10.1111/gcbb.12283 PG 12 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DE2XU UT WOS:000370492100013 ER PT J AU Szigeti, R Kellermayer, R AF Szigeti, Reka Kellermayer, Richard TI The forgotten yeast model of Hailey-Hailey disease SO INTERNATIONAL JOURNAL OF DERMATOLOGY LA English DT Letter ID HEIMLER-SYNDROME C1 [Szigeti, Reka] Dept Pathol & Immunol, St Louis, MO USA. [Kellermayer, Richard] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr,Baylor Coll Med, Texas Childrens Hosp,Dept Pediat,Sect Pediat Gast, Houston, TX 77030 USA. RP Kellermayer, R (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr,Baylor Coll Med, Texas Childrens Hosp,Dept Pediat,Sect Pediat Gast, Houston, TX 77030 USA. EM kellerma@bcm.edu NR 5 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0011-9059 EI 1365-4632 J9 INT J DERMATOL JI Int. J. Dermatol. PD MAR PY 2016 VL 55 IS 3 BP E169 EP E170 PG 2 WC Dermatology SC Dermatology GA DD8CE UT WOS:000370151100015 PM 26517976 ER PT J AU Tomasula, PM Sousa, AMM Liou, SC Li, R Bonnaillie, LM Liu, LS AF Tomasula, P. M. Sousa, A. M. M. Liou, S. -C. Li, R. Bonnaillie, L. M. Liu, L. S. TI Short communication: Electrospinning of casein/pullulan blends for food-grade application SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE nanotechnology; fiber; protein; polysaccharide ID RHEOLOGICAL PROPERTIES; NANOFIBERS; CASEINATE; PULLULAN; FILMS; DISPERSIONS; MEMBRANES; PROTEIN; FIBERS; FABRICATION AB Electrospinning is a complex process that produces fibers with diameters on the micrometer or nano-scale from an electrified jet of a polymer solution. The objective of this study was to create electrospun fibers for food use from aqueous solutions of calcium (CaCAS) or sodium caseinate (NaCAS). Fibers were not formed from electrospinning of solutions of either caseinate (CAS) at 50 degrees C, but were formed from blends of either CAS solution with aqueous solutions of the food-grade polysaccharide, pullulan (PUL), when using mass ratios from 2:1 to 1:4 of PUL/CAS. The CAS in the spinning solutions ranged from 3 to 15% (wt/wt) and the PUL ranged from 5 to 15% (wt/wt). The PUL/CaCAS 1:2 fibers showed the lowest fiber diameter sizes (FDS) of 172 +/- 43 nm, as determined by scanning electron microscopy, and were smaller in size than fibers electrospun from 15% (wt/wt) PUL solution. The PUL/NaCAS solutions were more viscous and formed fibers with occasional branching and less uniform FDS at higher NaCAS contents. Reductions in NaCAS in these solutions reduced viscosity and improved jet stabilities with consequent improvement in fiber morphology leading to more uniform FDS. Fibers with less defects and more homogeneous FDS were formed from PUL/CaCAS blends with more CaCAS, showing that each CAS interacted differently with PUL and formed the best fibers at different solution conditions. Calcium bridging may also underlie the anomalous behavior of the PUL/CaCAS blends by forming crosslinks with the phosphoserine residues, further enabling chain entanglements for fiber formation. The PUL/NaCAS fibers tended to be larger than the PUL/CaCAS fibers, which may also be due to other factors such as solution surface tension and conductivity, which also affect fiber quality and size. The shear viscosities at 100 s(-1) of the solutions producing fibers were within the range of 0.07 to 0.16 Pa/s, with the smallest standard deviations in FDS noted for solutions with viscosities within about 25% that of PUL. This is the first example of caseinate fibers prepared using a food-grade carrier rendering a product with potential use in food and packaging applications. C1 [Tomasula, P. M.; Sousa, A. M. M.; Liou, S. -C.; Li, R.; Bonnaillie, L. M.; Liu, L. S.] ARS, Dairy & Funct Foods Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Liou, S. -C.] Chung Shan Med Univ, 110,Sec 1,Jianguo N Rd, Taichung 402, Taiwan. [Li, R.] Tianjin Polytech Univ, Sch Mat Sci & Engn, State Key Lab Hollow Fiber Mat & Proc, 399 Binshuixi Rd, Tianjin 300387, Peoples R China. RP Tomasula, PM (reprint author), ARS, Dairy & Funct Foods Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM peggy.tomasula@ars.usda.gov FU National Program 306, Dairy and Functional Foods Research Unit, USDA, ARS, ERRC Project "Improving the Sustainability and Quality of Food and Dairy Products from Manufacturing to Consumption via Process Modeling and Edible Packaging" [8072-41000-096] FX The authors thank Raymond Kwoczak for the technical assistance in the characterization of the spinning solutions and Joseph Uknalis for the SEM analysis (both of USDA, ARS, ERRC, Wyndmoor, PA). This study was funded under National Program 306, Dairy and Functional Foods Research Unit, USDA, ARS, ERRC Project 8072-41000-096 "Improving the Sustainability and Quality of Food and Dairy Products from Manufacturing to Consumption via Process Modeling and Edible Packaging." NR 35 TC 1 Z9 1 U1 13 U2 48 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2016 VL 99 IS 3 BP 1837 EP 1845 DI 10.3168/jds.2015-10374 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DD7JO UT WOS:000370100600016 PM 26805973 ER PT J AU Liang, Y Carroll, JA Ballou, MA AF Liang, Yu Carroll, Jeffery A. Ballou, Michael A. TI The digestive system of 1-week-old Jersey calves is well suited to digest, absorb, and incorporate protein and energy into tissue growth even when calves are fed a high plane of milk replacer SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE calf; digestibility; nutrition ID DAIRY CALVES; BODY-COMPOSITION; HOLSTEIN CALVES; PERFORMANCE; NUTRITION; RESPONSES; WEIGHT; BLOOD; RATES; FAT AB The objectives of the current study were to determine the apparent digestibilities of nitrogen, organic matter, ash, and energy as well as investigate the nitrogen retention of calves fed different planes of milk replacer nutrition during the first week of life. Twelve Jersey calves were blocked by body weight at birth and randomly assigned to either a high plane of nutrition (HPN) or low plane of nutrition (LPN) treatment. The HPN calves were offered 19.2 g of dry matter/kg of body weight of a 28% all-milk crude protein and 20% fat milk replacer. The LPN calves were fed 11.6 g of dry matter/kg of body weight of a 20% all-milk crude protein and 20% fat milk replacer. All calves were given 3 L of pooled colostrum within 1 h of birth after which they were assigned to treatments; no starter was offered during the study. Calves were given 1 d to adapt to their treatments and environment, so calves were 30 to 36 h old at the start of data collection. The study was divided into two 72-h periods. Total collection of feces occurred over each 72 h period, and total urine was collected for the last 24 h of each period. Peripheral blood samples were collected at the beginning and end of each period and analyzed for plasma glucose and urea nitrogen concentrations. Data are reported as HPN vs. LPN, respectively. Fecal scores were greater for HPN calves during both periods; however, no difference was found in the dry matter percentage of feces (30:9 vs. 31.9 +/- 0.06). No differences were found between treatments in either digestible or metabolizable energy efficiencies, which averaged 93.3 and 83.7%, respectively. A treatment x period interaction was found on the percentage of intake nitrogen retained, in which calves fed the HPN had a greater percentage of intake nitrogen retained during period 1 (87.9 vs. 78.4 +/- 1.79%), but was not different from calves fed the LPN during period 2 (85.4 vs. 84.9 +/- 1.79%). From these data therefore, we conclude that healthy neonatal calves have the capability to digest and absorb the additional nutrients with a higher plane of nutrition during the first week of postnatal life. C1 [Liang, Yu; Ballou, Michael A.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79403 USA. [Carroll, Jeffery A.] ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. [Liang, Yu; Ballou, Michael A.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79403 USA. [Carroll, Jeffery A.] ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. RP Ballou, MA (reprint author), Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79403 USA.; Ballou, MA (reprint author), Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79403 USA. EM michael.ballou@ttu.edu FU Texas Animal Nutrition Council FX This research was funded by a grant from the Texas Animal Nutrition Council (txanc.org). The milk replacer for the study was donated by Land O'Lakes Animal Protein Co. of Shoreview, MN. The authors thank Tyler Harris with Texas Tech University and Jeff Dailey with USDA-ARS Livestock Issues Research Unit for their help collecting the data. NR 21 TC 1 Z9 1 U1 5 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2016 VL 99 IS 3 BP 1929 EP 1937 DI 10.3168/jds.2015-9895 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DD7JO UT WOS:000370100600025 PM 26723121 ER PT J AU Hall, MB AF Hall, Mary Beth TI Technical note: A method for isolating glycogen granules from ruminal protozoa for further characterization SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE rumen; glycogen; protozoa AB Evaluation of physical, chemical, and enzymatic hydrolysis characteristics of protozoal glycogen is best performed on a pure substrate to avoid interference from other cell components. A method for isolating protozoal glycogen granules without use of detergents or other potentially contaminating chemicals was developed. Rumen inoculum was incubated anerobically in vitro with glucose. Glycogen-laden protozoa produced in the fermentation, primarily isotrichids, were allowed to sediment in a separatory funnel and were dispensed. The protozoa were processed through repeated centrifugations and sonication to isolate glycogen granules largely free of feed and cellular debris. The final water-insoluble lyophilized product analyzed as 98.3% alpha-glucan with very rare starch granules and 1.9% protein. Observed losses of glycogen granules during the clean-up process indicate that this procedure should not be used for quantitative assessment of protozoal glycogen from fermentations. Further optimization of this procedure to enhance the amount of glycogen obtained per fermentation may be possible. C1 [Hall, Mary Beth] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. RP Hall, MB (reprint author), ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. EM marybeth.hall@ars.usda.gov FU USDA, Agricultural Research Service under National Program 101 Food Animal Production FX Special thanks go to the staff at the University of Wisconsin Laboratory for Optical and Computational Instrumentation for assistance with polarized light microscopy, and to J. W. Pitas and P. J. Weimer, US Dairy Forage Research Center, USDA-ARS, Madison, Wisconsin, for analytical assistance. This research was supported by funding from USDA, Agricultural Research Service under National Program 101 Food Animal Production. NR 14 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2016 VL 99 IS 3 BP 1956 EP 1958 DI 10.3168/jds.2015-10357 PG 3 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DD7JO UT WOS:000370100600029 PM 26805977 ER PT J AU Tunick, MH Thomas-Gahring, A Van Hekken, DL Iandola, SK Singh, M Qi, PX Ukuku, DO Mukhopadhyay, S Onwulata, CI Tomasula, PM AF Tunick, Michael H. Thomas-Gahring, Audrey Van Hekken, Diane L. Iandola, Susan K. Singh, Mukti Qi, Phoebe X. Ukuku, Dike O. Mukhopadhyay, Sudarsan Onwulata, Charles I. Tomasula, Peggy M. TI Physical and chemical changes in whey protein concentrate stored at elevated temperature and humidity SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE whey protein concentrate; color; powder flow; volatiles ID FUNCTIONAL-PROPERTIES; STORAGE-TEMPERATURE; VOLATILE COMPOUNDS; WATER ACTIVITY; DAIRY POWDERS; MILK POWDERS; STABILITY; FAT; FLOWABILITY; ISOLATE AB In a case study, we monitored the physical properties of 2 batches of whey protein concentrate (WPC) under adverse storage conditions to provide information on shelf life in hot, humid areas. Whey protein concentrates with 34.9 g of protein/100 g (WPC34) and 76.8 g of protein/100 g (WPC80) were stored for up to 18 mo under ambient conditions and at elevated temperature and relative humidity. The samples became yellower with storage; those stored at 35 degrees C were removed from the study by 12 mo because of their unsatisfactory appearance. Decreases in lysine and increases in water activity, volatile compound formation, and powder caking values were observed in many specimens. Levels of aerobic mesophilic bacteria, coliforms, yeast, and mold were <3.85 log(10) cfu/g in all-samples. Relative humidity was not a factor in most samples. When stored in sealed bags, these samples of WPC34 and WPC80 had a shelf life of 9 mo at 35 degrees C but at least 18 mo at lower temperatures, which should extend the market for these products. C1 [Tunick, Michael H.; Thomas-Gahring, Audrey; Van Hekken, Diane L.; Iandola, Susan K.; Qi, Phoebe X.; Onwulata, Charles I.; Tomasula, Peggy M.] ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Singh, Mukti] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Ukuku, Dike O.] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Mukhopadhyay, Sudarsan] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Tunick, MH (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM michael.tunick@ars.usda.gov NR 41 TC 0 Z9 0 U1 7 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2016 VL 99 IS 3 BP 2372 EP 2383 DI 10.3168/jds.2015-10256 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DD7JO UT WOS:000370100600070 PM 26778305 ER PT J AU VanRaden, PM AF VanRaden, P. M. TI Practical implications for genetic modeling in the genomics era SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE genetic evaluation; genomic selection; mixed models; multitrait ID DAIRY-CATTLE; REFERENCE POPULATION; RELATIONSHIP MATRIX; HOLSTEIN CATTLE; TRAIT; PREDICTION; REGRESSION; RELIABILITY; INCREASES; SELECTION AB Genetic models convert data into estimated breeding values and other information useful to breeders. The goal is to provide accurate and timely predictions of the future performance for each animal (or embryo). Modeling involves defining traits, editing raw data, removing environmental effects, including genetic by environmental interactions and correlations among traits, and accounting for nonadditive inheritance or nonnormal distributions. Data include phenotypes and pedigrees during the last century and genotypes within the last decade. The genomic data can include single nucleotide polymorphisms, quantitative trait loci, insertions, deletions, and haplotypes. Subsets must be selected to reduce computation because total numbers of variants that can be imputed have increased rapidly from thousands to millions. Current computation using 60,671 markers takes just a few days. Nonlinear models can account for the nonnormal distribution of genomic effects, but reliability is usually better than that of linear models only for traits influenced by major genes. Numbers of genotyped animals have also increased rapidly in the joint North American database from a few thousand in 2009 to over 1 million in 2015. Most are young females and will contribute to estimating allele effects in the future, but only about 150,000 have phenotypes so far. Genomic preselection can bias traditional animal models because Mendelian sampling of phenotyped progeny and mates is no longer expected to average zero; however, estimates of bias are small in current US data. Single-step models that combine pedigree and genomic relationships can account for preselection, but approximations are required for affordable computation. Traditional animal models may include all breeds and crossbreds, but most genomic evaluations are still computed within breed. Models that include inbreeding, heterosis, dominance, and interactions can improve predictions for individual matings. Multitrait genomic models may be preferred for traits with many missing records or when foreign records are included as pseudo-observations, but most countries use multitrait traditional evaluations followed by single-trait genomic evaluations. Genomic reliabilities are about 70% for the more heritable traits. Researchers must choose from many available models and explain how the models work so that breeders can more confidently apply the predictions in their selection programs. C1 [VanRaden, P. M.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP VanRaden, PM (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM Paul.VanRaden@ars.usda.gov FU USDA's Agricultural Research Service [1265-31000-096-00] FX The author thanks the Council on Dairy Cattle Breeding (Bowie, MD) for supplying data under USDA Nonfunded Cooperative Agreement 58-1245-3-228N; S. M. Hubbard, J. R. Wright, and M. E. Tooker (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) for technical manuscript review and contributions to the research; and 3 anonymous reviewers for many helpful comments. The figure was created using Daniel's XL Toolbox add in for Excel (version 6.60) by Daniel Kraus (Wurzburg, Germany). This research was supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information," of the USDA's Agricultural Research Service. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 46 TC 0 Z9 0 U1 3 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2016 VL 99 IS 3 BP 2405 EP 2412 DI 10.3168/jds.2015-10038 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DD7JO UT WOS:000370100600073 PM 26778313 ER PT J AU Chesnais, JP Cooper, TA Wiggans, GR Sargolzaei, M Pryce, JE Miglior, F AF Chesnais, J. P. Cooper, T. A. Wiggans, G. R. Sargolzaei, M. Pryce, J. E. Miglior, F. TI Using genomics to enhance selection of novel traits in North American dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE genetic evaluation; novel trait; prediction accuracy; genomics; selection ID IMMUNE-RESPONSE TRAITS; PRODUCER-RECORDED DATA; GENETIC-PARAMETERS; CANADIAN HOLSTEINS; MIDINFRARED SPECTROMETRY; RELATIONSHIP MATRIX; FEED-EFFICIENCY; BREEDING VALUES; JERSEY CATTLE; HEALTH TRAITS AB The objectives of this paper were to briefly review progress in the genetic evaluation of novel traits in Canada and the United States, assess methods to predict selection accuracy based on cow reference populations, and illustrate how the use of indicator traits could increase genomic selection accuracy. Traits reviewed are grouped into the following categories: udder health, hoof health, other health traits, feed efficiency and methane emissions, and other novel traits. The status of activities expected to lead to national genetic evaluations is indicated for each group of traits. For traits that are more difficult to measure or expensive to collect, such as individual feed intake or immune response, the development of a cow reference population is the most effective approach. Several deterministic methods can be used to predict the reliability of genomic evaluations based on cow reference population size, trait heritability, and other population parameters. To provide an empirical validation of those methods, predicted accuracies were compared with observed accuracies for several cow reference populations and traits. Reference populations of 2,000 to 20,000 cows were created through random sampling of genotyped Holstein cows in Canada and the United States. The effects of single nucleotide polymorphisms (SNP) were estimated from those cow records, after excluding the dams of validation bulls. Bulls that were first progeny tested in 2013 and 2014 were then used to carry out a validation and estimate the observed accuracy of genomic selection based on those SNP effects. Over the various cow population sizes and traits considered in the study, even the best prediction methods were found, on average, to either under-evaluate observed accuracy by 0.20 or over-evaluate it by 0.22, depending on' the approach used to estimate the number of independently segregating chromosome segments. In some instances, differences between observed and predicted accuracies were as large as 0.47. Indicator traits can be very useful for the selection of novel traits. To illustrate this, protein yield, body weight, and mid-infrared data were used as indicator traits for feed efficiency. Using those traits in conjunction with 5,000 cow records for dry matter intake increased the reliability of genomic predictions for young animals from 0.20 to 0.50. C1 [Chesnais, J. P.; Sargolzaei, M.] Semex Alliance, Guelph, ON N1G 3Z2, Canada. [Cooper, T. A.; Wiggans, G. R.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Sargolzaei, M.] Univ Guelph, Dept Anim Biosci, Ctr Genet Improvement Livestock, Guelph, ON N1G 2W1, Canada. [Pryce, J. E.] Dept Econ Dev Jobs Transport & Resources, Bundoora, Vic 3083, Australia. [Miglior, F.] Canadian Dairy Network, Guelph, ON N1K 1E5, Canada. RP Chesnais, JP (reprint author), Semex Alliance, Guelph, ON N1G 3Z2, Canada. EM jpchesnais@rogers.com FU USDA Agricultural Research Service [1245-31000-101-00] FX The authors thank the Canadian Dairy Network (Guelph, ON, Canada) and the Council on Dairy Cattle Breeding (Bowie, MD) for providing the data used in this paper, and S. M. Hubbard (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) for her kind input in manuscript editing and literature review. T. A. Cooper and G. R. Wiggans were supported by USDA Agricultural Research Service appropriated project 1245-31000-101-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information." NR 80 TC 5 Z9 5 U1 5 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAR PY 2016 VL 99 IS 3 BP 2413 EP 2427 DI 10.3168/jds.2015-9970 PG 15 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DD7JO UT WOS:000370100600074 PM 26778318 ER PT J AU Chandra, A Keizerweerd, AT Grisham, MP AF Chandra, Amaresh Keizerweerd, Amber T. Grisham, Michael P. TI Detection of Puccinia kuehnii Causing Sugarcane Orange Rust with a Loop-Mediated Isothermal Amplification-Based Assay SO MOLECULAR BIOTECHNOLOGY LA English DT Article DE Orange rust; LAMP; Puccinia kuehnii; Sugarcane; Bst DNA polymerase ID 1ST REPORT; CAUSAL AGENT; RAPID DETECTION; RT-LAMP; VIRUS; DNA AB Puccinia kuehnii is a fungal pathogen that causes orange rust in sugarcane, which is now prevalent in many countries. At the early stage of disease, it is almost indistinguishable from brown rust, which is caused by Puccinia melanocephala. Although several PCR assays are available to detect these diseases, the loop-mediated isothermal amplification (LAMP)-based assay has been reported to be more economical and easier to perform. Under isothermal conditions, DNA is amplified with high specificity and rapidity. Moreover, visual judgment of color change without further post-amplification processing makes the method convenient. The present study was undertaken to detect P. kuehnii genomic DNA using four primers corresponding to a unique DNA sequence of P. kuehnii. The LAMP assay was found to be optimal when 8 mM MgSO4 was used and the reaction was incubated at 63 A degrees C for 90 min. Positive samples showed a color change from orange to green upon SYBR Green I dye addition. Specificity of the LAMP test was checked with DNA of P. melanocephala, which showed no reaction. Sensitivity of the LAMP method was observed to be the same as real-time PCR at 0.1 ng, thus providing a rapid and more affordable option for early disease detection. C1 [Chandra, Amaresh; Keizerweerd, Amber T.; Grisham, Michael P.] ARS, Sugarcane Res Unit, USDA, Houma, LA 70360 USA. [Chandra, Amaresh] Indian Inst Sugarcane Res, Lucknow 226002, Uttar Pradesh, India. RP Keizerweerd, AT (reprint author), ARS, Sugarcane Res Unit, USDA, Houma, LA 70360 USA. EM amaresh_chandra@rediffmail.com; amber.keizerweerd@ars.usda.gov; michael.grisham@ars.usda.gov FU Department of Biotechnology, Government of India, New Delhi, India [05/AC/2012-12]; United States Department of Agriculture, Agriculture Research Service (CRIS) [6410-22000-013-00D] FX Amaresh Chandra is grateful to the Department of Biotechnology, Government of India, New Delhi, India for awarding the DBT-CREST Fellowship (BT/IN/DBT-CREST Awards/05/AC/2012-12). Amber Keizerweerd and Michael Grisham are thankful for financial support provided by the United States Department of Agriculture, Agriculture Research Service (CRIS Project: 6410-22000-013-00D). Thanks are also due to Kathryn Warnke for her technical support in conducting the real-time PCR assay. We also thank Dr. Duncan Clark of OptiGene Limited for his assistance in primer design. NR 26 TC 2 Z9 2 U1 1 U2 5 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 1073-6085 EI 1559-0305 J9 MOL BIOTECHNOL JI Mol. Biotechnol. PD MAR PY 2016 VL 58 IS 3 BP 188 EP 196 DI 10.1007/s12033-016-9914-5 PG 9 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DD9VT UT WOS:000370274500005 PM 26837389 ER PT J AU Novick, KA Miniat, CF Vose, JM AF Novick, Kimberly A. Miniat, Chelcy F. Vose, James M. TI Drought limitations to leaf-level gas exchange: results from a model linking stomatal optimization and cohesion-tension theory SO PLANT CELL AND ENVIRONMENT LA English DT Article DE stomatal conductance; transpiration; isohydric; anisohydric; water use efficiency; capacitance ID VAPOR-PRESSURE DEFICIT; MESOPHYLL DIFFUSION CONDUCTANCE; HYDRAULIC SAFETY MARGINS; MAXIMUM CARBON GAIN; WATER-USE; PINUS-TAEDA; ATMOSPHERIC CO2; BIOCHEMICAL LIMITATIONS; CANOPY TRANSPIRATION; PLANT PHOTOSYNTHESIS AB We merge concepts from stomatal optimization theory and cohesion-tension theory to examine the dynamics of three mechanisms that are potentially limiting to leaf-level gas exchange in trees during drought: (1) a demand limitation' driven by an assumption of optimal stomatal functioning; (2) hydraulic limitation' of water movement from the roots to the leaves; and (3) non-stomatal' limitations imposed by declining leaf water status within the leaf. Model results suggest that species-specific economics' of stomatal behaviour may play an important role in differentiating species along the continuum of isohydric to anisohydric behaviour; specifically, we show that non-stomatal and demand limitations may reduce stomatal conductance and increase leaf water potential, promoting wide safety margins characteristic of isohydric species. We used model results to develop a diagnostic framework to identify the most likely limiting mechanism to stomatal functioning during drought and showed that many of those features were commonly observed in field observations of tree water use dynamics. Direct comparisons of modelled and measured stomatal conductance further indicated that non-stomatal and demand limitations reproduced observed patterns of tree water use well for an isohydric species but that a hydraulic limitation likely applies in the case of an anisohydric species. This work represents a novel merging of concepts from stomatal optimization theory and cohesion-tension theory to examine the mechanisms that are potentially limiting to leaf gas exchange during drought. The model is used to identify distinguishing characteristics of demand limitations' to gas exchange imposed by atmospheric vapour pressure deficit, hydraulic limitations' to gas exchange imposed by the plant's need to prevent excessive cavitation and non-stomatal limitations' to gas exchange imposed by declining leaf water status. We demonstrate that these features are frequently detected in observations of tree water use and that demand and non-stomatal limitations may reduce stomatal conductance and increase leaf water potential, promoting wide safety margins characteristic of isohydric species. The coherent mapping of the model predictions to the empirical observations described in this study is encouraging and can inform and motivate ongoing efforts to explore how these different mechanisms jointly or independently regulate leaf-level gas exchange during drought. C1 [Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. [Miniat, Chelcy F.] USDA, Forest Serv, Coweeta Hydrol Lab, Otto, NC 28734 USA. [Vose, James M.] N Carolina State Univ, USDA, Ctr Integrated Forest Sci, Dept Forestry & Environm Res,Forest Serv,Southern, Raleigh, NC 27695 USA. RP Novick, KA (reprint author), Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. EM knovick@indiana.edu OI Miniat, Chelcy/0000-0002-3266-9783 FU US Department of Agriculture Forest Service, Southern Research Station FX We thank a number of individuals who assisted with data collection and analysis, including Rick Stagg, James Guldin, Mike Gavazzi, Steve McNulty, Jim Clark, David Bell, Becky Roper, Lindsey Boring, Jason McGee and in particular Barry Clinton, Daniel McInnis and Chris Sobek. We thank Stefano Manzoni and Rick Meinzer for providing valuable comments on earlier versions of the manuscript. This study was supported by the US Department of Agriculture Forest Service, Southern Research Station. The authors declare that they have no conflict of interest, financial or otherwise, that might be perceived as influencing their objectivity with respect to this work. NR 74 TC 6 Z9 6 U1 14 U2 56 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD MAR PY 2016 VL 39 IS 3 BP 583 EP 596 DI 10.1111/pce.12657 PG 14 WC Plant Sciences SC Plant Sciences GA DD7XI UT WOS:000370137900010 PM 26466749 ER PT J AU Peng, YC Gallegos, SA Gardner, DJ Han, Y Cai, ZY AF Peng, Yucheng Gallegos, Sergio A. Gardner, Douglas J. Han, Yousoo Cai, Zhiyong TI Maleic anhydride polypropylene modified cellulose nanofibril polypropylene nanocomposites with enhanced impact strength SO POLYMER COMPOSITES LA English DT Article ID MECHANICAL-PROPERTIES; FIBER COMPOSITES; COUPLING AGENTS; SURFACE; MORPHOLOGY; EXTRUSION; WHISKERS; FRACTURE; POLYMER; DEFORMATION AB Development of cellulose nanofibrils (CNFs) reinforced polypropylene (PP) nanocomposites using melt compounding processes has received considerable attention. The main challenges are to obtain well-dispersed CNFs in the polymer matrix and to establish compatible linkages between the CNFs and PP. Manufacturing of CNF reinforced PP nanocomposites was conducted using a twin-screw co-rotating extruder with the masterbatch concept. Modifications of CNFs using maleic anhydride polypropylene were performed. The best mechanical properties of the nanocomposites are 1.94 GPa (tensile modulus), 32.8 MPa (tensile strength), 1.63 GPa (flexural modulus), 50.1 MPa (flexural strength), and 3.8 kJ m(-2) (impact strength), which represents about 36, 11, 21, 7, and 23% improvement, respectively, compared to those of pure PP (1.43 GPa, 29.5 MPa, 1.35 GPa, 46.9 MPa, and 3.1 kJ m(-2)). Fracture morphology examination indicated good dispersion of CNFs in the PP matrix was achieved through this specific manufacturing process. MAPP treatments enhanced the interfacial adhesion between the CNFs and PP. POLYM. COMPOS., 37:782-793, 2016. (c) 2014 Society of Plastics Engineers C1 [Peng, Yucheng] Clemson Univ, Dept Mat Sci & Engn, Clemson, SC 29634 USA. [Gallegos, Sergio A.] Univ Concepcion, Chem Engn, Ave Cordillera 2634 Parque Ind Coronel, Coronel, Chile. [Gardner, Douglas J.; Han, Yousoo] Univ Maine, Adv Struct & Composites Ctr, Orono, ME 04469 USA. [Gardner, Douglas J.; Han, Yousoo] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Peng, YC (reprint author), Clemson Univ, Dept Mat Sci & Engn, Clemson, SC 29634 USA. EM yucheng@clemson.edu FU Maine Agricultural and Forestry Experiment Station Mclntire-Stennis Project [ME0-M-8-00527-13] FX Contract grant sponsor: Maine Agricultural and Forestry Experiment Station Mclntire-Stennis Project; contract grant number: ME0-M-8-00527-13. NR 60 TC 4 Z9 4 U1 12 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-8397 EI 1548-0569 J9 POLYM COMPOSITE JI Polym. Compos. PD MAR PY 2016 VL 37 IS 3 BP 782 EP 793 DI 10.1002/pc.23235 PG 12 WC Materials Science, Composites; Polymer Science SC Materials Science; Polymer Science GA DE2XF UT WOS:000370490200015 ER PT J AU Dow, CB Collins, BM Stephens, SL AF Dow, Christopher B. Collins, Brandon M. Stephens, Scott L. TI Incorporating Resource Protection Constraints in an Analysis of Landscape Fuel-Treatment Effectiveness in the Northern Sierra Nevada, CA, USA SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Treatment optimization; Burn probability; Emissions; Fuel treatments; Mixed conifer ID MIXED-CONIFER FOREST; REDUCTION TREATMENTS; FIRE BEHAVIOR; WESTERN US; TREATMENT IMPACTS; WILDLAND FIRES; PONDEROSA PINE; CARBON STOCKS; UNITED-STATES; SPOTTED OWL AB Finding novel ways to plan and implement landscape-level forest treatments that protect sensitive wildlife and other key ecosystem components, while also reducing the risk of large-scale, high-severity fires, can prove to be difficult. We examined alternative approaches to landscape-scale fuel-treatment design for the same landscape. These approaches included two different treatment scenarios generated from an optimization algorithm that reduces modeled fire spread across the landscape, one with resource-protection constrains and one without the same. We also included a treatment scenario that was the actual fuel-treatment network implemented, as well as a no-treatment scenario. For all the four scenarios, we modeled hazardous fire potential based on conditional burn probabilities, and projected fire emissions. Results demonstrate that in all the three active treatment scenarios, hazardous fire potential, fire area, and emissions were reduced by approximately 50 % relative to the untreated condition. Results depict that incorporation of constraints is more effective at reducing modeled fire outputs, possibly due to the greater aggregation of treatments, creating greater continuity of fuel-treatment blocks across the landscape. The implementation of fuel-treatment networks using different planning techniques that incorporate real-world constraints can reduce the risk of large problematic fires, allow for landscape-level heterogeneity that can provide necessary ecosystem services, create mixed forest stand structures on a landscape, and promote resilience in the uncertain future of climate change. C1 [Dow, Christopher B.; Stephens, Scott L.] Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Collins, Brandon M.] USDA, Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Berkeley, CA 94720 USA. RP Dow, CB (reprint author), Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM dowzer3@berkeley.edu NR 72 TC 1 Z9 1 U1 3 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD MAR PY 2016 VL 57 IS 3 BP 516 EP 530 DI 10.1007/s00267-015-0632-8 PG 15 WC Environmental Sciences SC Environmental Sciences & Ecology GA DD8SY UT WOS:000370198400002 PM 26614351 ER PT J AU Lauvaux, CA Skinner, CN Taylor, AH AF Lauvaux, Catherine Airey Skinner, Carl N. Taylor, Alan H. TI High severity fire and mixed conifer forest-chaparral dynamics in the southern Cascade Range, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Mixed conifer forest; Montane chaparral; Shrubs; Fire effects; Alternate stable states; Fire exclusion; Heterogeneity; Cascade Range; Vegetation shift ID NORTHERN SIERRA-NEVADA; VOLCANIC-NATIONAL-PARK; LAKE TAHOE BASIN; MONTANE FORESTS; LANDSCAPE PATTERN; KLAMATH MOUNTAINS; WILDERNESS AREAS; PRESCRIBED FIRE; WILDLAND FIRES; BURN SEVERITY AB Understanding how alternative vegetation types co-exist in a landscape is important in managing for biodiversity within an ecosystem. In California, mixed conifer forest is often interrupted by stands of shrubs known as montane chaparral. The development of chaparral stands following recent high severity or stand-replacing wildfires in mixed conifer forests has been well documented. Fire has been excluded from mixed conifer forests for over a century, and fuel loads are at historically high levels across much of this landscape. Despite contemporary post-fire research on mixed conifer forest, little is known about montane chaparral fire regimes or forest-chaparral dynamics in an ecosystem with a functioning fire regime. This study quantifies fire regimes in chaparral and adjacent forest and determines how chaparral responded to fire before fire exclusion in Lassen Volcanic National Park, California, a park that was never logged. Chaparral stems regenerated immediately after high severity fires in the 19th and early 20th century, and stem recruitment continued until the present. Fire return intervals in chaparral were longer than in adjacent forest (25 years vs. 11 years), and chaparral fires occurred during drier, potentially more extreme conditions. The apparent maintenance of stands of chaparral by less frequent, more severe fires suggests chaparral represents a self-reinforcing alternative stable state to forest. Following fire exclusion, chaparral stands gradually converted to forest as trees progressively invaded chaparral from the forest edge. Forest developing in chaparral is denser and more fir-enriched than the adjacent forest, similar to the understory that develops beneath a pine overstory following fire exclusion. Replacement of chaparral by forest reduces mixed conifer forest landscape diversity. However, the mixture of shrubs and trees in long unburned former chaparral is likely to burn with high severity effects in a subsequent fire. Since chaparral is also establishing in recent, very large high severity burn patches, chaparral extent may be expanding in the new configuration. If the decades needed for trees to invade from forest at the edge of severe burns exceed the fire return interval, chaparral may emerge as an alternative stable state to forest. Consequently, developing management strategies to increase resilience of mixed conifer forests to altered fire regimes is a pressing management challenge. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lauvaux, Catherine Airey; Taylor, Alan H.] Penn State Univ, Dept Geog, 302 Walker Bldg, University Pk, PA 16802 USA. [Lauvaux, Catherine Airey; Taylor, Alan H.] Penn State Univ, Interdept Grad Program Ecol, 302 Walker Bldg, University Pk, PA 16802 USA. [Skinner, Carl N.] US Forest Serv, USDA, Pacific Southwest Res Stn, 3644 Avetech Pkwy, Redding, CA 96002 USA. RP Taylor, AH (reprint author), Penn State Univ, Dept Geog, 302 Walker Bldg, University Pk, PA 16802 USA.; Taylor, AH (reprint author), Penn State Univ, Interdept Grad Program Ecol, 302 Walker Bldg, University Pk, PA 16802 USA. EM aht1@psu.edu FU National Park Service [H4560030064, H399206006] FX This research was conducted with support from the National Park Service under cooperative agreements H4560030064 and H399206006. The authors would like to thank the fire management staff at LVNP, particularly T. Garcia, E. Hensel and C. Ferris. We thank C. Pickett, K. Biondo, A. Synder, J. Poler, and D. Molinaro for assistance in the field. NR 105 TC 8 Z9 8 U1 20 U2 77 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2016 VL 363 BP 74 EP 85 DI 10.1016/j.foreco.2015.12.016 PG 12 WC Forestry SC Forestry GA DD7KU UT WOS:000370104200008 ER PT J AU Lorenz, TJ Vierling, KT Kozma, JM Millard, JE AF Lorenz, Teresa J. Vierling, Kerri T. Kozma, Jeffrey M. Millard, Janet E. TI Foraging plasticity by a keystone excavator, the white-headed woodpecker, in managed forests: Are there consequences for productivity? SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forage site selection; Nest productivity; Picoides albolarvatus; Pinus ponderosa; Ponderosa pine ID OPPORTUNISTIC BREEDER; LOXIA-CURVIROSTRA; EASTERN CASCADES; RED CROSSBILL; NEST SURVIVAL; DRY FORESTS; RESTORATION; PHYLOGENY; SELECTION; BEHAVIOR AB Information on the foraging ecology of animals is important for conservation and management, particularly for keystone species whose presence affects ecosystem health. We examined foraging by an at-risk cavity excavator, the white-headed woodpecker (Picoides albolarvatus). The foraging needs of this species are used to inform management of ponderosa pine (Pinus ponderosa) forests in some areas of western North America. Past observational studies indicated that white-headed woodpeckers forage predominately on cones and trunks of large-diameter (>68 cm) pines in old-growth stands, although habitat selection while foraging has not been formally examined. We used radio telemetry to track forage substrate use among 37 adult, breeding woodpeckers for 176 h (10,576 min) in forest stands that had been recently thinned and/or burned with prescribed fire. We used discrete choice models to examine forage site selection and multinomial regression to examine consequences of foraging on nest productivity. Woodpeckers foraged on more than ten individual substrates and switched substrates seasonally, presumably to take advantage of prey availability. Dead wood and fir foliage were used commonly in the nesting period (86% and 68% of foraging, respectively), whereas pine foliage and trunk foraging dominated in the fledgling (66% of foraging) and post-fledgling periods (73% of foraging). Average size of used trees was 49 cm (+/- 20 cm) and pine cones were rarely used (4% of foraging). During the nesting period, substrate use (chi(2) = 1.49, df = 4, P = 0.83) and distances traveled from nests for foraging did not affect productivity (F-(3,F-16) = 0.61, P = 0.62), which was high even for birds with the longest (2.1 km) and shortest (0.39 km) maximum forage distances. Habitats selected for foraging matched substrate use, and woodpeckers selected areas with low basal areas of live trees in the nesting period, but high basal areas in the post-nesting period. The variable foraging that we observed suggests that white-headed woodpeckers are plastic in their foraging in managed forests, and this plasticity has no negative consequences for productivity. Published by Elsevier B.V. C1 [Lorenz, Teresa J.; Vierling, Kerri T.] Univ Idaho, Dept Fish & Wildlife Sci, POB 441136, Moscow, ID 83844 USA. [Kozma, Jeffrey M.] Yakama Nation Timber Fish & Wildlife Fisheries Re, POB 151, Toppenish, WA 98942 USA. [Millard, Janet E.] US Forest Serv, USDA, 600 Sherbourne St, Leavenworth, WA 98826 USA. RP Lorenz, TJ (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM tlorenz@fs.fed.us RI Vierling, Kerri/N-6653-2016 FU U.S. Department of Agriculture, Forest Service FX This work was supported by the U.S. Department of Agriculture, Forest Service. We appreciate logistical support from R. Huffman with the Washington Department of Fish and Wildlife, and A. Lyons, K. Mellen-McLean, J. St. Hilaire, and D. Youkey with the U.S. Forest Service. J. Dudley, M. Kissling, C. Raley, M. Raphael, and P. Singleton loaned us equipment. We are grateful to individuals who provided advice on capturing woodpeckers and transmitter attachment, including R. Dixon, J. Dudley, C. Lehman, C. Raley, C. Rota, M. Rumble, R. Scott, and C. Werner, and to T. Johnson for providing advice on statistical methods. P. Fischer, K. Heniff, and S. Graham helped with field data collection, and P. Fischer was especially helpful in preparing transmitters and nets for trapping. C. Coffin, T. Bass, and J. Ashbaugh allowed access to their land. NR 57 TC 0 Z9 0 U1 10 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2016 VL 363 BP 110 EP 119 DI 10.1016/j.foreco.2015.12.021 PG 10 WC Forestry SC Forestry GA DD7KU UT WOS:000370104200011 ER PT J AU Dunbar-Irwin, M Safford, H AF Dunbar-Irwin, Mila Safford, Hugh TI Climatic and structural comparison of yellow pine and mixed-conifer forests in northern Baja California (Mexico) and the eastern Sierra Nevada (California, USA) SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Jeffrey pine; Fire history; Dry conifer forest; Sierra de San Pedro Martir; Baja California; Sierra Nevada ID SAN-PEDRO-MARTIR; TEMPERATURE LAPSE RATES; YOSEMITE-NATIONAL-PARK; GROWTH JEFFREY PINE; FIRE REGIMES; NORTHWESTERN MEXICO; SOUTHERN CASCADES; WESTERN MEXICO; SEVERITY; MORTALITY AB Effects of fire suppression policies on semi-arid yellow pine and mixed conifer (YPMC) forests in the western US have been well documented, and restoration of forest structure and natural fire regimes are high management priorities to ensure the health and resilience of such forests. However, determining reference conditions for ecological restoration is difficult due to the near absence of undegraded forests in the US. YPMC forests of the Sierra de San Pedro Martir (SSPM) in northern Baja California, Mexico, are highly similar to forests of the Eastern Sierra Nevada, California, USA, have experienced little to no logging, and until relatively recently supported a natural fire regime. As such, these Mexican YPMC forests are thought by many to represent reference ecosystems for restoration and resource and fire management in the US. However, to this point there has been no direct climatic comparison to determine to what extent SSPM is validly compared to California YPMC sites, nor a direct statistical comparison of forest conditions to see in what ways northern California forests might differ from SSPM. We compared climatic data from SSPM with 17 meteorological stations in the range of Jeffrey pine in Alta and Baja California. Based on this comparison, we determine that SSPM clearly belongs to the general class of Jeffrey pine dominated YPMC forests found along the eastern edge of the California Floristic Province. We used field sampling to measure forest structure, fuels, and vegetation and ground cover in SSPM and in multiple National Forests along the eastern slope of the Sierra Nevada. Live tree density was nearly twice as high in the eastern Sierra Nevada as in SSPM, and dead tree density was 2.6 times higher. Basal area was about 30% higher in the eastern Sierra Nevada, even though average tree size was larger in SSPM. Fuel loads and coarse woody debris were very similar between the two sites, and fine fuels (1-hour fuels) were actually higher in SSPM. Logging and fire suppression have resulted in denser YPMC forests dominated by smaller trees in the US, but our results suggest that fire suppression in SSPM over the last 30 years has increased fuel loads. Nonetheless, the Baja California forests still retain an overstory structure created and maintained by centuries of frequent fire. This study provides important reference information for the management of eastern Sierra Nevada forests, and indicates that continued full fire suppression in SSPM carries significant ecological risks. Published by Elsevier B.V. C1 [Dunbar-Irwin, Mila] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Safford, Hugh] US Forest Serv, USDA, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. [Safford, Hugh] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. RP Safford, H (reprint author), US Forest Serv, USDA, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. EM mdunbarirwin@gmail.com; hughsafford@fs.fed.us FU US Forest Service International Programs; US Forest Service Pacific Southwest Region Ecology Program; UC-Davis Ecology Graduate Group; UC-Davis Plant Sciences Department; Comision Nacional de Areas Naturales Protegidas (CONANP) FX Funding was provided by US Forest Service International Programs, the US Forest Service Pacific Southwest Region Ecology Program, the UC-Davis Ecology Graduate Group, the UC-Davis Plant Sciences Department, and the Comision Nacional de Areas Naturales Protegidas (CONANP). Field crews in the SSPM included Zayra Pena, Jessica Trujillo, Ibes Davila, and Hiram Rivera from Mexico; and John Williams, Kevin Hickenbottom, Haley Wiggins, and Ed Kentner from the US. In the ESN, Clark Richter, Amy Jirka, Amy Sturgill, Maggie Mansfield, Haley Wiggins, and Deb Scolnick carried out most of the field work. Botany help was provided by Onkar Singh and Ed Kentner. At UC-Davis, members of the Young Lab provided theoretical and analytical ideas and editing, with particular support from Gabrielle Bohlman. Brendan Barrett provided extensive statistical help. Truman Young edited many drafts of the manuscript. NR 88 TC 1 Z9 1 U1 6 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 1 PY 2016 VL 363 BP 252 EP 266 DI 10.1016/j.foreco.2015.12.039 PG 15 WC Forestry SC Forestry GA DD7KU UT WOS:000370104200025 ER PT J AU Nam, WH Hong, EM Choi, JY AF Nam, Won-Ho Hong, Eun-Mi Choi, Jin-Yong TI Assessment of water delivery efficiency in irrigation canals using performance indicators SO IRRIGATION SCIENCE LA English DT Article ID DECISION-SUPPORT; MANAGEMENT; SYSTEM; RIVER; PRODUCTIVITY; PROJECT; LEVEL; KOREA; CONSERVATION; TURKEY AB Agricultural water is delivered by open irrigation canals in system of reservoirs with a widespread distribution in South Korea. Traditional irrigation management problems include water distribution systems with less capacity than the peak demand, irregular delivery rates, and low irrigation efficiency and uniformity. It is necessary to strategically compare the estimated irrigation demands with the actual water supplies for decision making in order to maintain the water supply according to the demand. Accurate measurement and monitoring of water distribution systems is essential in order to solve the problems of water efficiency and availability. Auto water level gauges installed at the head and tail sections of each irrigation canal in the Dongjin River were used to measure the discharge during irrigation periods. In this study, we introduced an approach to assess the water delivery performance indicators of the open irrigation canals, which is essential for identifying the key issues for water management improvement. The irrigation efficiencies according to the water delivery performance indicators were measured with an automatic water gauge in the irrigation canals and were calculated from the spatial and temporal distribution of the water supply for the lack of planning in water delivery. The calculated performance indicators are useful to understand the irrigator behavior and general irrigation trends. Analysis of the results yielded insights into possible improvement methods in order to develop water management policies that enable irrigation planners to improve the temporal uniformity and equity in the water distribution. C1 [Nam, Won-Ho] Univ Nebraska, Natl Drought Mitigat Ctr, Sch Nat Resources, Lincoln, NE USA. [Hong, Eun-Mi] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Choi, Jin-Yong] Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Rural Syst Engn, Seoul, South Korea. RP Hong, EM (reprint author), USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM silbern7@snu.ac.kr OI NAM, WONHO/0000-0002-9671-6569 FU Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2013R1A6A3A03019009]; Rural Research Institute of Korea Rural Community Corporation by the Ministry of Agriculture, Food and Rural Affairs FX This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A6A3A03019009), and Rural Research Institute of Korea Rural Community Corporation by the Ministry of Agriculture, Food and Rural Affairs. The views expressed in this paper are those of the authors and do not necessarily reflect the views of NRF or any of its sub-agencies. NR 39 TC 3 Z9 3 U1 2 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAR PY 2016 VL 34 IS 2 BP 129 EP 143 DI 10.1007/s00271-016-0488-6 PG 15 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DD6QD UT WOS:000370047800004 ER PT J AU Beck, BH Li, C Farmer, BD Barnett, LM Lange, MD Peatman, E AF Beck, B. H. Li, C. Farmer, B. D. Barnett, L. M. Lange, M. D. Peatman, E. TI A comparison of high- and low-virulence Flavobacterium columnare strains reveals differences in iron acquisition components and responses to iron restriction SO JOURNAL OF FISH DISEASES LA English DT Article DE columnaris disease; Flavobacterium columnare; iron uptake; virulence ID TONB AB Flavobacterium columnare, the causative agent of columnaris disease causes substantial mortality worldwide in numerous freshwater finfish species. Due to its global significance, an improved understanding of the factors that contribute to virulence is urgently needed. In a laboratory challenge, we found that significantly greater mortality was observed in channel catfish Ictalurus punctatus (Rafinesque) challenged with isolate LSU-066-04 (LSU) as compared to fish challenged with isolate LV-359-01 (LV). Strikingly, mortality was 100% in LSU-challenged fish, with all fish dying within the first 24h after challenge, while mortality in the LV-challenged group was significantly lower with 26.7% of fish dying on days 1-4 post-challenge. There were no differences in initial bacterial adhesion between the isolates at 1-2h post-challenge; however, by 4h LSU-challenged fish had a greater bacterial load on the gill. Next, to better understand this variation in virulence, we examined transcriptional and functional attributes related to iron acquisition. The isolates were differentially sensitive to iron restriction both in vitro and in vivo and the basal expression of TonB family member genes and a ferroxidase gene differed significantly. Our findings provide new insight into iron uptake and pathogen virulence, and offer promising new targets for columnaris prevention and treatment. C1 [Beck, B. H.; Farmer, B. D.; Barnett, L. M.; Lange, M. D.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Li, C.; Peatman, E.] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. RP Beck, BH (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM benjamin.beck@usda.ars.gov NR 51 TC 1 Z9 1 U1 1 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD MAR PY 2016 VL 39 IS 3 BP 259 EP 268 DI 10.1111/jfd.12343 PG 10 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA DD6WF UT WOS:000370064600001 PM 25704170 ER PT J AU Xu, DH Zhang, QZ Zhang, D AF Xu, D-H Zhang, Q-Z Zhang, D. TI Two in vitro methods for screening potential parasiticides against Ichthyophthirius multifiliis using Tetrahymena thermophila SO JOURNAL OF FISH DISEASES LA English DT Article DE Ichthyophthirius multifiliis; in vitro; lethal concentrations; parasiticides; screen; Tetrahymena thermophila ID CYPRINUS-CARPIO L; MALACHITE GREEN; CHANNEL CATFISH; FOUQUET; FISH; PYRIFORMIS; INFECTION; ASSAYS; CILIOPHORA; TOXICITY AB Ichthyophthirius multifiliis (Ich) is a ciliate parasite that infects many species of freshwater fishes worldwide and causes heavy economic losses in aquaculture. Currently, parasiticides for controlling this parasite are limited, and few pond-practical chemical therapies exist. Hence, the search for new parasiticides is urgently needed. One challenge confronting the screening of potential parasiticides is the difficulty in raising enough parasite for efficacy testing as Ich is an obligate parasite. This study used species of Tetrahymena, Ich-related and cultivable ciliate protozoa, to evaluate two in vitro methods to test parasiticides. Plate counting and MTS assays (CellTiter 96((R)) AQ(ueous) Non-Radioactive Cell Proliferation Assay) were used to compare lethal concentrations or median lethal concentrations (LC50) of copper sulphate, formalin and malachite green between T.thermophila and Ich theronts or between T.thermophila and Ich tomonts. The parasiticides that killed T.thermophila have been demonstrated to kill theronts or tomonts. These in vitro methods using T.thermophila can be used to screen novel parasiticides against Ich. C1 [Xu, D-H; Zhang, D.] ARS, Aquat Anim Hlth Res Unit, USDA, Auburn, AL 36832 USA. [Zhang, Q-Z] Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China. RP Xu, DH (reprint author), ARS, Aquat Anim Hlth Res Unit, USDA, 990 Wire Rd, Auburn, AL 36832 USA. EM dehai.xu@ars.usda.gov FU USDA-ARS CRIS [6420-32000-024-00D] FX The authors are grateful to Dr Victor Panangala, USDA, ARS, Aquatic Animal Health Research Unit, and Dr Chao Li, Qingdao Agricultural University, Qingdao, China, for valuable comments to improve the quality of this manuscript. This research was supported by USDA-ARS CRIS No. 6420-32000-024-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 34 TC 0 Z9 0 U1 2 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD MAR PY 2016 VL 39 IS 3 BP 285 EP 294 DI 10.1111/jfd.12361 PG 10 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA DD6WF UT WOS:000370064600004 PM 25857201 ER PT J AU Qiu, D Xu, LS Vandemark, G Chen, WD AF Qiu, Dan Xu, Liangsheng Vandemark, George Chen, Weidong TI Comparative Transcriptome Analysis between the Fungal Plant Pathogens Sclerotinia sclerotiorum and S. trifoliorum Using RNA Sequencing SO JOURNAL OF HEREDITY LA English DT Article DE RNA sequencing; Sclerotinia sclerotiorum; S. trifoliorum; transcriptome ID GENES; PH; OXALATE; TOOL; SEQ AB The fungal plant pathogens Sclerotinia sclerotiorum and S. trifoliorum are morphologically similar, but differ considerably in host range. In an effort to elucidate mechanisms of the host range difference, transcriptomes of the 2 species at vegetative growth stage were compared to gain further insight into commonality and uniqueness in gene expression and pathogenic mechanisms of the 2 closely related pathogens. A total of 23 133 and 21 043 unique transcripts were obtained from S. sclerotiorum and S. trifoliorum, respectively. Approximately 43% of the transcripts were genes with known functions for both species. Among 1411 orthologous contigs, about 10% (147) were more highly (>3-fold) expressed in S. trifoliorum than in S. sclerotiorum, and about 12% (173) of the orthologs were more highly (>3-fold) expressed in S. sclerotiorum than in S. trifoliorum. The expression levels of genes on the supercontig 30 have the highest correlation coefficient value between the 2 species. Twenty-seven contigs were found to be new and unique for S. trifoliorum. Additionally, differences in expressed genes involved in pathogenesis like oxalate biosynthesis and endopolygalacturonases were detected between the 2 species. The analyses of the transcriptomes not only discovered similarities and uniqueness in gene expression between the 2 closely related species, providing additional information for annotation the S. sclerotiorum genome, but also provided foundation for comparing the transcriptomes with host-infecting transcriptomes. C1 [Qiu, Dan; Xu, Liangsheng] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Vandemark, George; Chen, Weidong] Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res, Pullman, WA 99164 USA. [Qiu, Dan] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Qiu, Dan] Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO 65211 USA. RP Chen, WD (reprint author), Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res, Pullman, WA 99164 USA. EM w-chen@wsu.edu FU USDA ARS National Sclerotinia Initiative FX USDA ARS National Sclerotinia Initiative (partial support). NR 35 TC 1 Z9 1 U1 2 U2 21 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 EI 1465-7333 J9 J HERED JI J. Hered. PD MAR PY 2016 VL 107 IS 2 BP 163 EP 172 DI 10.1093/jhered/esv092 PG 10 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DD6VY UT WOS:000370063900008 PM 26615185 ER PT J AU Ager, AA Day, MA Short, KC Evers, CR AF Ager, Alan A. Day, Michelle A. Short, Karen C. Evers, Cody R. TI Assessing the impacts of federal forest planning on wildfire risk mitigation in the Pacific Northwest, USA SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Forest restoration; Wildland urban interface; Wildfire exposure; Risk transmission; Firesheds ID WILDLAND-URBAN INTERFACE; UNITED-STATES; ECOLOGICAL RESTORATION; LANDSCAPE RESTORATION; ECOSYSTEM SERVICES; FIRE; MANAGEMENT; SIMULATION; EXPOSURE; TRANSMISSION AB We analyzed the impact of amenity and biodiversity protection as mandated in national forest plans on the implementation of hazardous fuel reduction treatments aimed at protecting the wildland urban interface (WUI) and restoring fire resilient forests. We used simulation modeling to delineate areas on national forests that can potentially transmit fires to adjacent WUI. We then intersected these areas with national forest planning maps to determine where mechanical treatments are allowed for restoration and fire protection, versus areas where they are prohibited. We found that a large proportion of the national forest lands (79%) can spawn fires that burn adjacent WUIs. The bulk of the predicted WUI exposure originated from simulated fires ignited outside of conservation and preservation reserves and in dry forests, rather than moist mixed conifer forests. Thus the notion that fuel buildup in reserves on national forests contributes to wildfire risk in the urban interface was only partially supported by the data for the region studied. Most of the national forest lands that contribute wildfires to the WUI are not within the boundaries of community wildfire protection plans, which may undermine the effectiveness of these planning efforts. We used the spatial data themes developed in the study to map conflicts and opportunities for restoration and mitigation of WUI wildfire risk. The analysis disentangles the spatial complexity of managing landscapes for multiple socio-ecological objectives as part of ongoing restoration programs, collaborative planning, and national forest plan revisions on national forests in the US. (C) 2015 Elsevier B.V. All rights reserved. C1 [Ager, Alan A.; Short, Karen C.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 US Highway 10 W, Missoula, MT 59808 USA. [Day, Michelle A.] Oregon State Univ, Coll Forestry Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Evers, Cody R.] Portland State Univ, Dept Environm Sci & Management, POB 751, Portland, OR 97207 USA. RP Ager, AA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 US Highway 10 W, Missoula, MT 59808 USA. EM aager@fs.fed.us; michelle.day@oregonstate.edu; kcshort@fs.fed.us; cevers@pdx.edu FU Forest Service Region 6 Natural Resources program area, Portland, OR; Joint Fire Sciences Program under JFSP [14-1-01-22] FX We thank Kent Connaughton, former regional forester for the Pacific Northwest Region for many discussions about strategies for prioritizing restoration investments in the Pacific Northwest region. We also thank John Laurence for his comments on an earlier version of the manuscript. This work was funded by the Forest Service Region 6 Natural Resources program area, Portland, OR, and the Joint Fire Sciences Program under JFSP project no. 14-1-01-22. NR 87 TC 4 Z9 4 U1 8 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD MAR PY 2016 VL 147 BP 1 EP 17 DI 10.1016/j.landurbplan.2015.11.007 PG 17 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA DD7JM UT WOS:000370100300001 ER PT J AU Troy, A Nunery, A Grove, JM AF Troy, Austin Nunery, Ashley Grove, J. Morgan TI The relationship between residential yard management and neighborhood crime: An analysis from Baltimore City and County SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Crime; Environmental design; Residential landscaping; Yards; Spatial analysis ID INNER-CITY; GREEN SPACE; URBAN; VEGETATION; ENVIRONMENT; COMMUNITY; PATTERNS; CANOPY; STRESS; FEAR AB We analyzed the relationship between crime and indicators of residential yard management in Baltimore City and County. Data came from a survey we conducted of over one thousand front yards that included more than 40 indicators relating to lawns, trees, shrubs, beds and other features. These indicators were related to point counts of crime at the 150 m scale using a combination of ordinary least squares, spatial error, and Poisson regressions. After controlling for income, population density, block-scale tree canopy, and housing type, we found a consistently significant relationship between crime and a number of indicators of yard management. Yard-level variables that were negatively associated with crime included: the presence of yard trees, garden hoses/sprinklers, and lawns, in addition to the percentage of pervious area in a yard. Those positively associated with crime included presence of litter, desiccation of the lawn, lack of cutting of the lawn, and number of small trees in front of or adjacent to the property. While these results do not establish causality, they add evidence to a growing literature that suggests the possibility of several mechanisms by which environmental design may reduce crime: "cues to care" (the inverse of the "broken window" hypothesis) can lead to reduced crime by signaling to criminals the presence of social capital and the active involvement of neighbors in community spaces; and more appealing landscaping draws more "eyes on the street," which in turn deters criminals. (C) 2015 Elsevier B.V. All rights reserved. C1 [Troy, Austin] Univ Colorado, Denver Campus Box 126,POB 173364, Denver, CO 80217 USA. [Nunery, Ashley] Univ Vermont, Burlington, VT 05405 USA. [Grove, J. Morgan] US Forest Serv, USDA, Washington, DC USA. RP Troy, A (reprint author), Univ Colorado, Denver Campus Box 126,POB 173364, Denver, CO 80217 USA. EM austin.troy@ucdenver.edu; ashley.lidman@gmail.com; morgangrove@fs.fed.us FU U.S. Forest Service's Northern Research Station and Northeastern Area State & Private Forestry Program [USDA03-CA-11244225-531]; National Science Foundation Long-Term Ecological Research program [NSF DEB-0423476]; National Science Foundation Human and Social Dynamics Program [0624159] FX This research was made possible by the generous support of: the U.S. Forest Service's Northern Research Station and Northeastern Area State & Private Forestry Program (USDA03-CA-11244225-531), the National Science Foundation Long-Term Ecological Research program (NSF DEB-0423476) and the National Science Foundation Human and Social Dynamics Program (award #0624159). Many thanks also go out to Bryant Smith, Allison Colwell, and Chris Dubin for their indispensable work in collecting field data, as well as Jarlath O'Neil-Dunne of the University of Vermont Spatial Analysis Lab, for his remote sensing work. We also wish to thank the Baltimore Ecosystem Study, which provided valuable research infrastructure. Finally, we wish to thank Colin Drane of Spotcrime for providing us with crime data and valuable guidance on that data. NR 40 TC 0 Z9 0 U1 11 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD MAR PY 2016 VL 147 BP 78 EP 87 DI 10.1016/j.landurbplan.2015.11.004 PG 10 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA DD7JM UT WOS:000370100300008 ER PT J AU Snyder, D Cernicchiaro, N Cohnstaedt, LW AF Snyder, D. Cernicchiaro, N. Cohnstaedt, L. W. TI Sugar-feeding status alters biting midge photoattraction SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Culicoides; attraction; light-emitting diodes; sugar feeding; ultraviolet; vision ID LIGHT-EMITTING-DIODES; SONORENSIS DIPTERA; CULICOIDES SPP.; TRAP EFFICIENCY; VISUAL-SYSTEM; CERATOPOGONIDAE; ORIENTATION; MOSQUITOS; RESPONSES; ULTRAVIOLET AB The biting midge Culicoides sonorensis Wirth and Jones (Diptera: Ceratopogonidae) transmits pathogens to both livestock and wildlife. Biting midge surveillance relies heavily on light traps for collection; however, little is known about the light spectra preferences of C. sonorensis midges. A light assay arena was constructed and light-emitting diodes (LEDs) of various light spectra were used as light sources to evaluate midge photoattraction. A comparison of responses to light spectra indicated the highest proportions of C. sonorensis were attracted to ultraviolet (UV) light and that midges differentiated 10-nm differences in wavelength. Stronger intensities of UV light resulted in greater attraction. Midges exhibited both sugar-seeking and escape behaviours under different conditions of sugar supplementation before and during the experiment. These behaviours occurred with lights of 355 nm and 365 nm in wavelength. Based on the results of this study, the attraction of C. sonorensis to light traps can be improved through the use of bright LEDs at 355 nm or 365 nm. C1 [Snyder, D.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Cernicchiaro, N.] Kansas State Univ, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Cohnstaedt, L. W.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Cohnstaedt, LW (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM Lee.Cohnstaedt@ars.usda.gov NR 39 TC 0 Z9 0 U1 2 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD MAR PY 2016 VL 30 IS 1 BP 31 EP 38 DI 10.1111/mve.12144 PG 8 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DD4EF UT WOS:000369874600005 PM 26555011 ER PT J AU Kamo, K Lakshman, D Pandey, R Guaragna, MA Okubara, P Rajasekaran, K Cary, J Jordan, R AF Kamo, Kathryn Lakshman, Dilip Pandey, Ruchi Guaragna, Mary Ann Okubara, Patricia Rajasekaran, Kanniah Cary, Jeffrey Jordan, Ramon TI Resistance to Fusarium oxysporum f. sp gladioli in transgenic Gladiolus plants expressing either a bacterial chloroperoxidase or fungal chitinase genes SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Floral monocots; Stable transformation; Fungus resistance ID NONHEME CHLOROPEROXIDASE; PSEUDOMONAS-PYRROCINIA; ENHANCED RESISTANCE; ENDOCHITINASE GENE; DISEASE RESISTANCE; TOMATO PLANTS; HEAD BLIGHT; WHEAT; TOBACCO; ANTIFUNGAL AB Three antifungal genes, a non-heme chloroperoxidase from Pseudomonas pyrrocinia, and an exochitinase and endochitinase from Fusarium venetanum under regulation by the CaMV 35S promoter, were used to transform Gladiolus for resistance to Fusarium oxysporum f. sp. gladioli. Gladiolus plants were confirmed to be transgenic by Southern hybridization. Semi-quantitative RT-PCR of RNA isolated from leaves and roots demonstrated expression of the Fusarium exochitinase and endochitinase genes in transgenic plants compared to controls. All transgenic plants expressing the Fusarium exochitinase or endochitinase gene had chitinase activity higher than that of the control plants. Semi-quantitative RT-PCR verified that three of the four plant lines with the chloroperoxidase gene expressed the transgene in leaves and roots while no expression was detected in control plants. Western hybridization confirmed the presence of the chloroperoxidase protein in both leaves and roots of transgenic plants. Cell extracts from one endochitinase plant line inhibited growth of germinated F. oxysporum spores more consistently than extracts from the four chloroperoxidase and three endochitinase plant lines. Three chloroperoxidase, two exochitinase, and three endochitinase transgenic plant lines sustained a significantly (P < 0.05) lower density of hyphae on roots compared to roots of non-transformed Gladiolus plants three to four days following exposure of the roots to Fusarium. Shoots from two plant lines, one containing a chloroperoxidase and the other an endochitinase gene, had less necrosis when rated on a scale of 1-3 and appeared visually to be healthier and without obvious Fusarium infection than non-transformed, regenerated Gladiolus plants 17-21 days following exposure to F. oxysporum. C1 [Kamo, Kathryn; Lakshman, Dilip; Pandey, Ruchi; Guaragna, Mary Ann; Jordan, Ramon] USDA, Floral & Nursery Plants Res Unit, Natl Arboretum, Beltsville, MD 20705 USA. [Okubara, Patricia] USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. [Rajasekaran, Kanniah; Cary, Jeffrey] USDA, Food & Feed Safety Res Unit, So Reg Res Ctr, New Orleans, LA USA. RP Kamo, K (reprint author), USDA, Floral & Nursery Plants Res Unit, Natl Arboretum, Beltsville, MD 20705 USA. EM Kathryn.Kamo@ars.usda.gov NR 47 TC 0 Z9 0 U1 6 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD MAR PY 2016 VL 124 IS 3 BP 541 EP 553 DI 10.1007/s11240-015-0913-1 PG 13 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DD5FA UT WOS:000369946900008 ER PT J AU Yatso, KN Lilleskov, EA AF Yatso, Kassidy N. Lilleskov, Erik A. TI Effects of tree leaf litter, deer fecal pellets, and soil properties on growth of an introduced earthworm (Lumbricus terrestris): Implications for invasion dynamics SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Lumbricus terrestris growth rates; Earthworm invasion; Tree leaf litter quality; Northern hardwood-hemlock forests; Deer pellets; Soil calcium ID DISSOLVED ORGANIC-MATTER; NORTHERN HARDWOOD FORESTS; FLUORESCENCE; CALCIUM; DECOMPOSITION; DISAPPEARANCE; SPECTROSCOPY; PALATABILITY; TEMPERATE; CHEMISTRY AB Invasive earthworm communities are expanding into previously earthworm-free forests of North America, producing profound ecosystem changes. Lumbricus terrestris is an invasive anecic earthworm that consumes a large portion of the detritus on the soil surface, eliminating forest floor organic horizons and reducing soil organic matter. Two mesocosm experiments were used to examine the individual and combined effects of litter and soil type on the growth of L. terrestris. The litter type experiment tested nine different food source treatments (7 tree leaf litters, deer fecal pellets, and a control), while the soil x litter type experiment used five different soil treatments (4 soil types and one soil type with A horizon material removed) in combination with four different food source treatments. We found that leaf litter type (p = 0.001) and soil type (p = 0.018) significantly affected earthworm growth rates, with growth rates on deer pellets similar to many high quality deciduous leaf litters. Of soil variables, exchangeable Ca (r(2) = 0.99), sum base cations (r(2) = 0.98), % organic matter (r(2) = 0.93), %N (r(2) = 0.89), %C (r(2) = 0.87), and exchangeable Mg (r(2) = 0.85) were all significant predictors of earthworm growth. Litter disappearance of all litter types was linearly related to growth, suggesting similar growth efficiency on different litter types. However, chemical properties, specifically foliar C:N and a linear combination of a suite of other chemical properties predicted growth, suggesting that consumption or gut passage rates were regulated by litter quality. This information about soil and litter characteristics that regulate L terrestris growth should improve models of their distribution, spread and abundance. Published by Elsevier Ltd. C1 [Yatso, Kassidy N.] Michigan Technol Univ, 1400 Townsend Dr, Houghton, MI 49931 USA. [Lilleskov, Erik A.] US Forest Serv, No Res Stn, 410 Maclnnes Dr, Houghton, MI 49931 USA. RP Lilleskov, EA (reprint author), US Forest Serv, No Res Stn, 410 Maclnnes Dr, Houghton, MI 49931 USA. EM kassidy.yatso@gmail.com; elilleskov@fs.fed.us FU Michigan Technological University Summer Undergraduate Research Fellowship (SURF) program; U.S. Forest Service, Northern Research Station FX This research was supported by a grant from the Michigan Technological University Summer Undergraduate Research Fellowship (SURF) program to KNY and by support from the U.S. Forest Service, Northern Research Station to KNY and EAL. Thanks to Kerry Woods and the Huron Mountain Wildlife Foundation for access to the Huron Mountain Club for soil and earthworm sampling. We thank N. Karberg for help with soil sampling and preparation. We are grateful to L. Potvin, K. Juneau, A. Quinn, K.J. Romanowicz IV, Tim Veverica, and K. Kinnear for their assistance during fieldwork and laboratory analysis. Thanks to Mac Callaham and Grizelle Gonzalez for insightful reviews of the manuscript. NR 52 TC 3 Z9 3 U1 11 U2 49 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD MAR PY 2016 VL 94 BP 181 EP 190 DI 10.1016/j.soilbio.2015.11.030 PG 10 WC Soil Science SC Agriculture GA DD7HK UT WOS:000370094100019 ER PT J AU Halvorson, JJ Schmidt, MA Hagerman, AE Gonzalez, JM Liebig, MA AF Halvorson, Jonathan J. Schmidt, Michael A. Hagerman, Ann E. Gonzalez, Javier M. Liebig, Mark A. TI Reduction of soluble nitrogen and mobilization of plant nutrients in soils from U.S northern Great Plains agroecosystems by phenolic compounds SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Phenolic compounds; Nutrient cycling; Soluble nitrogen; Hydrolyzable tannins; Phosphorus Soluble cations ID NATURAL ORGANIC-MATTER; COATED QUARTZ SAND; CONDENSED TANNINS; CROPPING SYSTEMS; MANGANESE OXIDES; COMPOUNDS DIFFER; TEMPERATE SOILS; CEREAL-GRAINS; FOREST SOILS; INITIAL PH AB Phenolic plant secondary metabolites actively participate in a broad range of important reactions that affect livestock, plants and soil. In soil, phenolic compounds can affect nutrient dynamics and mobility of metals but their role in northern Great Plains agroecosystems is largely unknown. We evaluated the effects of three phenolic compounds on plant nutrient extractability in agricultural soil. Soil samples (0-10 cm) from pasture and cropped sites near Mandan, North Dakota, USA were treated with water (control) or aqueous solutions of increasingly complex compounds; benzoic acid (BA), gallic acid (GA), or beta-1,2,3,4,6-penta-O-galloyl-D-glucose (PGG) at four concentrations (1.25, 2.5, 5 or 10 mg compound gram(-1) soil). We measured extractable nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and manganese (Mn) in treatment supernatants and after a subsequent incubation in hot water (16 h, 80 degrees C). While significant quantities of nitrogen were extracted with water, each compound reduced N extracted in a concentration dependent manner with greatest response to BA (about 25%). However, PGG reduced the solubility of N only during the hot water incubation, suggesting its effects are mostly on organic forms of N. Unlike N, GA and PGG increased extraction of P, relative to water, while BA had less effect. Extraction of the major cations, K, Ca, and Mg, was strongly increased by BA and GA but unaffected by PGG. Extraction of Mn was increased most by treatments of GA but less so by BA. The effects of PGG on Mn were consistent with GA when expressed on a molar basis. These findings suggest plant secondary compounds affect nutrient dynamics in soil, and thus may be part of future management strategies to improve nutrient-use efficiency. Published by Elsevier Ltd. C1 [Halvorson, Jonathan J.; Liebig, Mark A.] USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. [Schmidt, Michael A.] Wright State Univ, Dayton, OH 45435 USA. [Hagerman, Ann E.] Miami Univ, Oxford, OH 45056 USA. [Gonzalez, Javier M.] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Halvorson, JJ (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM Jonathan.Halvorson@ars.usda.gov NR 86 TC 0 Z9 0 U1 10 U2 25 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD MAR PY 2016 VL 94 BP 211 EP 221 DI 10.1016/j.soilbio.2015.11.022 PG 11 WC Soil Science SC Agriculture GA DD7HK UT WOS:000370094100022 ER PT J AU Headd, B Bradford, SA AF Headd, Brendan Bradford, Scott A. TI Use of aerobic spores as a surrogate for cryptosporidium oocysts in drinking water supplies SO WATER RESEARCH LA English DT Review DE Cryptosporidium oocysts; Aerobic spores; Groundwater; Surface water; GWUDI; Review ID SATURATED POROUS-MEDIA; BACILLUS-CEREUS SPORES; COLLOID FILTRATION THEORY; SECONDARY-MINIMUM DEPOSITION; ALLUVIAL GRAVEL AQUIFER; ORGANIC-MATTER SURFACES; PARVUM OOCYSTS; BACTERIAL-SPORES; ESCHERICHIA-COLI; STAINLESS-STEEL AB Waterborne illnesses are a growing concern among health and regulatory agencies worldwide. The United States Environmental Protection Agency has established several rules to combat the contamination of water supplies by cryptosporidium oocysts, however, the detection and study of cryptosporidium oocysts is hampered by methodological and financial constraints. As a result, numerous surrogates for cryptosporidium oocysts have been proposed by the scientific community and efforts are underway to evaluate many of the proposed surrogates. The purpose of this review is to evaluate the suitability of aerobic bacterial spores to serve as a surrogate for cryptosporidium oocysts in identifying contaminated drinking waters. To accomplish this we present a comparison of the biology and life cycles of aerobic spores and oocysts and compare their physical properties. An analysis of their surface properties is presented along with a review of the literature in regards to the transport, survival, and prevalence of aerobic spores and oocysts in the saturated subsurface environment. Aerobic spores and oocysts share many commonalities with regard to biology and survivability, and the environmental prevalence and ease of detection make aerobic spores a promising surrogate for cryptosporidium oocysts in surface and groundwater. However, the long-term transport and release of aerobic spores still needs to be further studied, and compared with available oocyst information. In addition, the surface properties and environmental interactions of spores are known to be highly dependent on the spore taxa and purification procedures, and additional research is needed to address these issues in the context of transport. Published by Elsevier Ltd. C1 [Headd, Brendan; Bradford, Scott A.] ARS, US Salin Lab, USDA, 450 W Big Springs Rd, Riverside, CA 92507 USA. RP Bradford, SA (reprint author), ARS, US Salin Lab, USDA, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Scott.Bradford@ars.usda.gov FU USDA, ARS [NP 214] FX This research was supported by the USDA, ARS, NP 214. We would to thank Philip Berger of the U.S. EPA for helpful comments and discussion concerning this manuscript. NR 261 TC 1 Z9 1 U1 4 U2 28 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD MAR 1 PY 2016 VL 90 BP 185 EP 202 DI 10.1016/j.watres.2015.12.024 PG 18 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DD7GP UT WOS:000370092000020 PM 26734779 ER PT J AU Han, Y Hwang, G Kim, D Bradford, SA Lee, B Eom, I Kim, PJ Choi, SQ Kim, H AF Han, Yosep Hwang, Gukhwa Kim, Donghyun Bradford, Scott A. Lee, Byoungcheun Eom, Igchun Kim, Pil Je Choi, Siyoung Q. Kim, Hyunjung TI Transport, retention, and long-term release behavior of ZnO nanoparticle aggregates in saturated quartz sand: Role of solution pH and biofilm coating SO WATER RESEARCH LA English DT Article DE ZnO nanoparticle aggregates; Transport and retention; Long-term release; Biofilm; Solution pH; Saturated quartz sand ID ZINC-OXIDE NANOPARTICLES; NATURAL ORGANIC-MATTER; HETEROGENEOUS POROUS-MEDIA; COLLOID FILTRATION THEORY; SECONDARY ENERGY MINIMUM; ESCHERICHIA-COLI O157H7; SURFACE-CHARGE; IONIC-STRENGTH; MARINE-PHYTOPLANKTON; BACTERIAL DEPOSITION AB The transport, retention, and long-term release of zinc oxide nanoparticle aggregates (denoted below as ZnO-NPs) were investigated in saturated, bare and biofilm (Pseudomonas putida) coated sand packed columns. Almost complete retention of ZnO-NPs occurred in bare and biofilm coated sand when the influent solution pH was 9 and the ionic strength (IS) was 0.1 or 10 mM NaCl, and the retention profiles were always hyper-exponential. Increasing the solution IS and biofilm coating produced enhanced retention of ZnO-NPs near the column inlet. The enhanced NPs retention at high IS was attributed to more favorable NP-silica and NP-NP interactions; this was consistent with the interaction energy calculations. Meanwhile, the greater NPs retention in the presence of biofilm was attributed to larger roughness heights which alter the mass transfer rate, the interaction energy profile, and lever arms associated with the torque balance; e.g., scanning electron and atomic force microscopy was used to determine roughness heights of 33.4 nm and 97.8 nm for bare sand and biofilm-coated sand, respectively. Interactions between NPs and extracellular polymeric substances may have also contributed to enhanced NP retention in biofilm-coated sand at low IS. The long-term release of retained ZnO-NPs was subsequently investigated by continuously injecting NP-free solution at pH 6, 9, or 10 and keeping the IS constant at 10 mM. The amount and rate of retained ZnO-NP removal was strongly dependent on the solution pH. Specifically, almost complete removal of retained ZnO-NPs was observed after 627 pore volumes when the solution pH was 6, whereas much less Zn was recovered when the eluting solution pH was buffered to pH = 9 and especially 10. This long-term removal was attributed to pH-dependent dissolution of retained ZnO-NPs because: (i) the solubility of ZnO-NPs increases with decreasing pH; and (ii) ZnO-NPs were not detected in the effluent. The presence of biofilm also decreased the initial rate and amount of dissolution and the subsequent transport of Zn2+ due to the strong Zn2+ re-adsorption to the biofilm. Our study indicates that dissolution will eventually lead to the complete removal of retained ZnO-NPs and the transport of toxic Zn2+ ions in groundwater environments with pH ranges of 5-9. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Han, Yosep; Hwang, Gukhwa; Kim, Donghyun; Kim, Hyunjung] Chonbuk Natl Univ, Dept Mineral Resources & Energy Engn, Jeonju 561756, Jeonbuk, South Korea. [Bradford, Scott A.] ARS, USDA, US Salin Lab, Riverside, CA 92507 USA. [Lee, Byoungcheun; Eom, Igchun; Kim, Pil Je] Natl Inst Environm Res, Risk Assessment Div, Hwangyeong Ro 42, Inchon 404708, South Korea. [Choi, Siyoung Q.] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Daejeon 305701, South Korea. RP Kim, H (reprint author), Chonbuk Natl Univ, Dept Mineral Resources & Energy Engn, Jeonju 561756, Jeonbuk, South Korea. EM kshjkim@jbnu.ac.kr FU National Institute of Environmental Research, Ministry of Environment of Korea, Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education [NRF-2015020766]; Ministry of Education (MOE); National Research Foundation of Korea (NRF) through the Human Resource Training Project for Regional Innovation [2015H1C1A1035930] FX This research was supported by the National Institute of Environmental Research, Ministry of Environment of Korea, Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2015020766), and the Ministry of Education (MOE) and National Research Foundation of Korea (NRF) through the Human Resource Training Project for Regional Innovation (2015H1C1A1035930). NR 66 TC 5 Z9 5 U1 15 U2 64 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD MAR 1 PY 2016 VL 90 BP 247 EP 257 DI 10.1016/j.watres.2015.12.009 PG 11 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DD7GP UT WOS:000370092000025 PM 26741396 ER PT J AU Kumar, A Mantovani, EE Seetan, R Soltani, A Echeverry-Solarte, M Jain, S Simsek, S Doehlert, D Alamri, MS Elias, EM Kianian, SF Mergoum, M AF Kumar, Ajay Mantovani, E. E. Seetan, R. Soltani, A. Echeverry-Solarte, M. Jain, S. Simsek, S. Doehlert, D. Alamri, M. S. Elias, E. M. Kianian, S. F. Mergoum, M. TI Dissection of Genetic Factors underlying Wheat Kernel Shape and Size in an Elite x Nonadapted Cross using a High Density SNP Linkage Map SO PLANT GENOME LA English DT Article ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; YIELD-RELATED TRAITS; RADIATION HYBRID MAPS; SPRING WHEAT; BREAD WHEAT; GRAIN-SIZE; D-GENOME; AEGILOPS-TAUSCHII; NATURAL VARIATION AB Wheat kernel shape and size has been under selection since early domestication. Kernel morphology is a major consideration in wheat breeding, as it impacts grain yield and quality. A population of 160 recombinant inbred lines (RIL), developed using an elite (ND 705) and a nonadapted genotype (PI 414566), was extensively phenotyped in replicated field trials and genotyped using Infinium iSelect 90K assay to gain insight into the genetic architecture of kernel shape and size. A high density genetic map consisting of 10,172 single nucleotide polymorphism (SNP) markers, with an average marker density of 0.39 cM/marker, identified a total of 29 genomic regions associated with six grain shape and size traits; similar to 80% of these regions were associated with multiple traits. The analyses showed that kernel length (KL) and width (KW) are genetically independent, while a large number (similar to 59%) of the quantitative trait loci (QTL) for kernel shape traits were in common with genomic regions associated with kernel size traits. The most significant QTL was identified on chromosome 4B, and could be an ortholog of major rice grain size and shape gene GS3 or qGL3. Major and stable loci also were identified on the homeologous regions of Group 5 chromosomes, and in the regions of TaGW2 (6A) and TaGASR7 (7A) genes. Both parental genotypes contributed equivalent positive QTL alleles, suggesting that the nonadapted germplasm has a great potential for enhancing the gene pool for grain shape and size. This study provides new knowledge on the genetic dissection of kernel morphology, with a much higher resolution, which may aid further improvement in wheat yield and quality using genomic tools. C1 [Kumar, Ajay; Mantovani, E. E.; Soltani, A.; Echeverry-Solarte, M.; Jain, S.; Simsek, S.; Doehlert, D.; Elias, E. M.; Kianian, S. F.; Mergoum, M.] N Dakota State Univ, Dept Plant Sci, POB 6050, Fargo, ND 58108 USA. [Seetan, R.] Slippery Rock Univ, Dept Comp Sci, Slippery Rock, PA 16057 USA. [Alamri, M. S.] King Saud Univ, Dept Food Sci & Nutr, POB 2460, Riyadh 11451, Saudi Arabia. [Kianian, S. F.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Mergoum, M (reprint author), N Dakota State Univ, Dept Plant Sci, POB 6050, Fargo, ND 58108 USA. EM Mohamed.Mergoum@ndsu.edu FU North Dakota Wheat Commission FX This study was funded by the North Dakota Wheat Commission. The authors would also like to thank J. Hegstad, A. Peckrul, J. Underdahl, M. Abdallah, A. Walz, in addition to the members of the hard red spring wheat and wheat germplasm enhancement projects for their excellent technical assistance. NR 77 TC 3 Z9 3 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2016 VL 9 IS 1 DI 10.3835/plantgenome2015.09.0081 PG 22 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DO9ZM UT WOS:000378146900017 ER PT J AU Rincker, K Hartman, GL Diers, BW AF Rincker, Keith Hartman, Glen L. Diers, Brian W. TI Fine Mapping of Resistance Genes from Five Brown Stem Rot Resistance Sources in Soybean SO PLANT GENOME LA English DT Article ID PHIALOPHORA-GREGATA; CEPHALOSPORIUM-GREGATUM; LINKAGE MAP; REGISTRATION; MARKERS; BSR-101; GENOME; YIELD AB Brown stem rot (BSR) of soybean [Glycine max (L.) Merr.] caused by Cadophora gregata (Allington & Chamb.) T.C. Harr. & McNew can be controlled effectively with genetic host resistance. Three BSR resistance genes Rbs1, Rbs2, and Rbs3, have been identified and mapped to a large region on chromosome 16. Marker-assisted selection (MAS) will be more efficient and gene cloning will be facilitated with a narrowed genomic interval containing an Rbs gene. The objective of this study was to fine map the positions of Rbs genes from five sources. Mapping populations were developed by crossing the resistant sources 'Bell', PI 84946-2, PI 437833, PI 437970, L84-5873, and PI 86150 with either the susceptible cultivar Colfax or Century 84. Plants identified as having a recombination event near Rbs genes were selected and individually harvested to create recombinant lines. Progeny from recombinant lines were tested in a C. gregata root-dip assay and evaluated for foliar and stem BSR symptom development. Overall, 4878 plants were screened for recombination, and progeny from 52 recombinant plants were evaluated with simple-sequence repeat (SSR) genetic markers and assessed for symptom development. Brown stem rot resistance was mapped to intervals ranging from 0.34 to 0.04 Mb in the different sources. In all sources, resistance was fine mapped to intervals inclusive of BARCSOYSSR_16_1114 and BARCSOYSSR_16_1115, which provides further evidence that one locus provides BSR resistance in soybean. C1 [Rincker, Keith; Diers, Brian W.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, USDA ARS, Soybean Maize Germplasm Pathol & Genet, Res Unit, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Diers, BW (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM bdiers@illinois.edu NR 42 TC 2 Z9 2 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2016 VL 9 IS 1 DI 10.3835/plantgenome2015.08.0063 PG 14 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DO9ZM UT WOS:000378146900014 ER PT J AU Ruttanaprasert, R Jogloy, S Vorasoot, N Kesmala, T Kanwar, RS Holbrook, CC Patanothai, A AF Ruttanaprasert, Ruttanachira Jogloy, Sanun Vorasoot, Nimitr Kesmala, Thawan Kanwar, Rameshwar S. Holbrook, C. Corley Patanothai, Aran TI Effects of water stress on total biomass, tuber yield, harvest index and water use efficiency in Jerusalem artichoke SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Drought; Available soil water; Health food; Inulin; Yield potential; Yield reduction ID DROUGHT TOLERANCE; WHOLE-PLANT; L.; GROWTH; PERFORMANCE; CLONES AB The objectives of this study were to determine the effects of drought on tuber yield, total biomass, harvest index, water use efficiency of tuber yield (WUEt) and water use efficiency of biomass (WUEb), and to evaluate the differential responses of Jerusalem artichoke (JA) varieties under drought stress. The 3 x 5 factorial combinations of three water regimes (Field capacity (FC), 50% available soil water (50%AW) and 25%AW), and five JA varieties (JA 60, JA 125, JA 5, JA 89 and HEL 65) were arranged in a pot experiment in a randomized complete block design with four replications for two years. Data were recorded for tuber dry weight, total biomass, harvest index, WUEt and WUEb at harvest. Drought reduced tuber dry weight, total biomass, harvest index, WUEt and WUEb, and reductions were more severe under the severe drought stress of 25%AW. Varieties were significantly different for all traits under drought and well-watered conditions. The JA varieties were classified into three groups. The first category was comprised of the JA 5 variety with high tuber yield potential and low drought tolerance, the second category consisted of JA 60 and JA 125 varieties with low tuber yield potential and high drought tolerance, and the third group included JA 89 and HEL 65 varieties with low tuber yield potential and low drought tolerance. The multiple regression analysis showed that tuber yield, total biomass and harvest index at 50%AW and 25%AW depended largely on the reductions of tuber yield, total biomass and harvest index under drought. Therefore, the results of this study recommend that the selection of JA genotypes with low reduction in yield under drought stress could be a criterion in drought resistance breeding programs for development of JA varieties with high tuber yield under drought stress. JA with drought tolerance in this study means high tuber yield under drought conditions. JA 5 had high yield and WUEt across water regimes and could be used as parental source for drought tolerance breeding programs in further research. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ruttanaprasert, Ruttanachira; Jogloy, Sanun; Vorasoot, Nimitr; Kesmala, Thawan; Patanothai, Aran] Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. [Jogloy, Sanun] Khon Kaen Univ, Peanut & Jerusalem Artichoke Improvement Funct Fo, Khon Kaen 40002, Thailand. [Kanwar, Rameshwar S.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. [Holbrook, C. Corley] USDA ARS, Coastal Plain Expt Stn, POB 748, Tifton, GA 31793 USA. RP Jogloy, S (reprint author), Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. EM sjogloy@gmail.com FU Royal Golden Jubilee Ph.D. Program [PHD/0026/2551]; Khon Kaen University; Thailand Research Fund [RTA 5880003, IRG 5780003]; Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University; Higher Education Promotion and National Research University Project of Thailand; office of Higher Education Commission; Food and Functional Food Research Cluster of Khon Kaen University; Khon Kaen University (KKU); Faculty of Agriculture, KKU FX We are very appreciative of the funds provided by the Royal Golden Jubilee Ph.D. Program (jointly funded by Khon Kaen University and the Thailand Research Fund) (grant no. PHD/0026/2551), the Thailand Research Fund for providing financial supports to this research through the Senior Research Scholar Project of Professor Dr. Sanun Jogloy (Project no. RTA 5880003), and also supported in part by the Plant Breeding Research Center for Sustainable Agriculture, Khon Kaen University. In addition, we are grateful to the Higher Education Promotion and National Research University Project of Thailand, office of Higher Education Commission, and the Food and Functional Food Research Cluster of Khon Kaen University for funding this study. Finally, we would like to thank the Thailand Research Fund (Porject no.IRG 5780003), Khon Kaen University (KKU) and Faculty of Agriculture, KKU for providing financial support for training on manuscript preparation. NR 31 TC 1 Z9 1 U1 6 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD MAR 1 PY 2016 VL 166 BP 130 EP 138 DI 10.1016/j.agwat.2015.12.022 PG 9 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DC8EZ UT WOS:000369454000013 ER PT J AU Shapiro-Ilan, DI Cottrell, TE Mizell, RF Horton, DL AF Shapiro-Ilan, David I. Cottrell, Ted E. Mizell, Russell F., III Horton, Dan L. TI Efficacy of Steinernema carpocapsae plus fire gel applied as a single spray for control of the lesser peachtree borer, Synanthedon pictipes SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Entomopathogenic nematode; Formulation; Gel; Peach; Steinernema carpocapsae; Synanthedon pictipes ID ENTOMOPATHOGENIC NEMATODES; APPLICATION TECHNOLOGY; PLUTELLA-XYLOSTELLA; BIOLOGICAL-CONTROL; LEPIDOPTERA; PESTS; ADJUVANTS; SESIIDAE AB The efficacy of aboveground applications of entomopathogenic nematodes (Heterorhabditis spp. and Steinernema spp.) can be severely limited by the nematode's susceptibility to UV radiation and desiccation. The lesser peachtree borer, Synanthedon pictipes, is a major pest of stone fruit; larvae attack trees aboveground by tunneling into the trunk and scaffold limbs. In previous research, Steinernema carpocapsae, caused high levels of S. pictipes mortality when a sprayable fire gel (Barricade (R)) was applied on top of the nematode application as a protectant. One drawback to the approach is that two applications must be made (first nematodes are applied followed by the fire gel); furthermore, the previous experiments did not compare nematode application to the existing standard chemical insecticide. Therefore, the objectives of this study were to (1) determine if a diluted rate of fire gel can protect nematodes when applied as a single spray, and (2) compare the efficacy of nematode applications with the chemical insecticide, chlorpyrifos. The experiment was conducted in a peach orchard in Quincy, Florida in 2013 and 2014. Treatments included: (1) chlorpyrifos, (2) S. carpocapsae applied in aqueous suspension only or (3) with a full rate (approximately 4% applied separately) or (4) 2% Barricade (R) (applied with nematodes in a single spray), and (5) a non-treated control. The treatments were applied post-harvest (in the fall) to S. pictipes-infested bark wounds; S. pictipes survival was assessed 8 (2013) or 14 (2014) d post-application. In 2013, chlorpyrifos and nematodes with Barricade (R) at 2% or the full rate reduced S. pictipes survival relative to the non-treated control and nematodes without Barricade (R). In 2014, nematodes applied with 2% Barricade (R) was the only treatment that reduced S. pictipes survival. We conclude that S. carpocapsae and Barricade (R) can be applied as a single spray, and in our experiments the treatment was at least as effective as the chemical standard. Published by Elsevier Inc. C1 [Shapiro-Ilan, David I.; Cottrell, Ted E.] USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. [Mizell, Russell F., III] Univ Florida, Dept Entomol, Quincy, FL USA. [Horton, Dan L.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. RP Shapiro-Ilan, DI (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM David.Shapiro@ars.usda.gov FU National Institute of Food and Agriculture, U.S. Department of Agriculture SARE (Sustainable Agriculture Research and Education) [LS11-241] FX The authors thank Merry Bacon, Ryan Burciaga, Stacy Byrd, Leigh Ann Coley, Charlie Riddle, and Saleah Starks for providing technical assistance. This study was supported in part by National Institute of Food and Agriculture, U.S. Department of Agriculture SARE (Sustainable Agriculture Research and Education) Grant No. LS11-241. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the United States Department of Agriculture. NR 23 TC 4 Z9 4 U1 3 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAR PY 2016 VL 94 BP 33 EP 36 DI 10.1016/j.biocontrol.2015.12.006 PG 4 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DC5AL UT WOS:000369232100004 ER PT J AU Yu, LX Setter, TL AF Yu, Long-Xi Setter, Tim L. TI Comparative transcriptomes between viviparous1 and wildtype maize developing endosperms in response to water deficit SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE DNA microarray; Kernel development; vp1 mutant; Drought stress; Transcript ID ABSCISIC-ACID; GENE-EXPRESSION; KERNEL DEVELOPMENT; ARABIDOPSIS; PATTERNS; CARBOHYDRATE; ACTIVATOR; ENCODES; GENOME; SET AB Maize kernel development is particularly sensitive to water stress at the early post-pollination phase. Endosperm plays an essential role in support of embryo development and represents the bulk of carbohydrate storage in the kernel. In the present study, we compared transcriptomes of developing maize endosperms between an ABA-insensitive mutant, viviparous1 (vp1), and wild type (wt) under water deficit. A total of 122 and 164 transcripts were significantly affected by water deficit in vp1 and wt, respectively. Transcript profiles indicated that the Vp1 transcription factor contributed to regulation in response to water stress at early stages of maize endosperm development. Genes involved in transcriptional regulation and signal transduction were particularly dependent on presence of a functional Vp1 allele, as 83% the stress-affected genes in these categories were up-regulated by water stress in the wt, but only 34% were up-regulated in the mutant. This suggests that the loss of ABA sensitivity altered signaling networks in response to water deficit such that the mutant was unable to up regulate the expression of many genes that normally play regulatory roles. Thus, the Vp1 gene plays a role in regulating transcript expression in maize endosperm development in response to water deficit, and this regulation is likely modulated via ABA signaling pathway. Published by Elsevier B.V. C1 [Yu, Long-Xi] ARS, Plant & Germplasm Intro & Testing Res, USDA, Prosser, WA USA. [Setter, Tim L.] Cornell Univ, Sect Soil & Crop Sci, Sch Integrat Plant Sci, Ithaca, NY USA. RP Yu, LX (reprint author), USDA ARS, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM longxi.yu@ars.usda.gov FU USDA-NRI Award [2003-35100-13305] FX This work was supported by the USDA-NRI Award No. 2003-35100-13305. NR 29 TC 0 Z9 0 U1 4 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD MAR PY 2016 VL 123 BP 116 EP 124 DI 10.1016/j.envexpbot.2015.11.009 PG 9 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA DD1JF UT WOS:000369677300012 ER PT J AU Hecht, LBB Thompson, PC Lavin, ES Zarlenga, DS Rosenthal, BM AF Hecht, Luke B. B. Thompson, Peter C. Lavin, Elizabeth S. Zarlenga, Dante S. Rosenthal, Benjamin M. TI Hybridization is limited between two lineages of freeze-resistant Trichinella during coinfection in a mouse model SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Hybridization; Assortative mating; Gene flow; Fitness; Parasite; Trichinella ID MULTIPLEX PCR; SPIRALIS; INTROGRESSION; SPECIATION; NATIVA; REINFORCEMENT; GENOTYPES; SYMPATRY; FLOW AB Hybridization between two closely related but distinct genetic lineages may lead to homogenization of the two lineages with potentially novel phenotypes, or selective pressure to avoid hybridization if the two lineages are truly distinct. Trichinella nativa and Trichinella T6 are zoonotic nematode parasites which can be distinguished genetically despite occasional hybridization. Here, using an experimental murine model, we attempt to determine whether there are barriers to hybridization when sizeable numbers of each lineage are allowed to coinfect a host. Two mice were independently infected with equal numbers of T. nativa and T6. The offspring of these coinfections were genotyped at two microsatellite loci and one mitochondrial locus capable of distinguishing T. nativa from T6 genotypes. Among larvae in the F1 generation, offspring of every possible mating were encountered. Most larvae ( 63.6%) derived from T. nativa x T. nativa matings, while 21.1% of offspring were the product of T6 x T6 matings, and only 15.3% were hybrid offspring of T. nativa x T6 crosses, differing markedly from null expectations. In this experimental model, T. nativa and Trichinella T6 were able to mate, but ratios of offspring indicated pre- or post-zygotic barriers to hybridization that may include assortative mating, genetic incompatibilities, and/or differences in the fitness of offspring. These barriers would limit gene flow between these two lineages in a natural setting, serving as a barrier to their homogenization and promoting their persistence as distinct and separate entities. (C) 2015 The Authors. Published by Elsevier B.V. C1 [Hecht, Luke B. B.; Thompson, Peter C.; Lavin, Elizabeth S.; Zarlenga, Dante S.; Rosenthal, Benjamin M.] ARS, Anim Parasit Dis Lab, USDA, BARC East, Bldg 1180, Beltsville, MD 20705 USA. [Lavin, Elizabeth S.] Univ Maryland, Coll Agr & Nat Resources, College Pk, MD 20742 USA. [Hecht, Luke B. B.] UCL Earth Sci, Kathleen Lonsdale Bldg,Gower St, London WC1E 6BT, England. RP Thompson, PC (reprint author), ARS, Anim Parasit Dis Lab, USDA, BARC East, Bldg 1180, Beltsville, MD 20705 USA. EM lbbhecht@gmail.com; pete.c.thompson@gmail.com; elavin@terpmail.umd.edu; dante.zarlenga@ars.usda.gov; benjamin.rosenthal@ars.usda.gov OI Thompson, Peter/0000-0002-9161-167X; Hecht, Luke/0000-0003-3884-4885; Rosenthal, Benjamin/0000-0002-0224-3773 FU USDA-ARS project [8042-42000-016-00D]; NIFA/AFRI [2011-67005-30348] FX We would like to thank Detiger Dunams-Morel for exceptional technical support and training that allowed this project to get off the ground. Additionally, we would like to thank four anonymous reviewers who greatly improved the focus and clarity of the manuscript. The experiments presented here were funded by USDA-ARS project 8042-42000-016-00D. PCT was supported by a training fellowship funded by NIFA/AFRI grant #2011-67005-30348, administered by the Oak Ridge Institute for Science and Education. NR 30 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 EI 1567-7257 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD MAR PY 2016 VL 38 BP 146 EP 151 DI 10.1016/j.meegid.2015.12.016 PG 6 WC Infectious Diseases SC Infectious Diseases GA DC4XJ UT WOS:000369223300025 PM 26721624 ER PT J AU Villasante, A Powell, MS Moutou, K Murdoch, GK Overturf, K Wacyk, J Hardy, RW AF Villasante, Alejandro Powell, Madison S. Moutou, Katerina Murdoch, Gordon K. Overturf, Ken Wacyk, Jurij Hardy, Ronald W. TI Effects of anthocyanidins on myogenic differentiation and antioxidant defense in primary myogenic cells isolated from rainbow trout (Oncorhynchus mykiss) SO AQUACULTURE LA English DT Article DE Anthocyanidins; Rainbow trout; Skeletal muscle; Myogenic cells; Myogenesis; Antioxidant ID MUSCLE STEM-CELLS; PLANT-PROTEIN-SOURCES; SKELETAL-MUSCLE; OXIDATIVE STRESS; SATELLITE CELLS; FISH-MEAL; HYDROGEN-PEROXIDE; GROWTH-PERFORMANCE; REGULATORY FACTORS; TOTAL REPLACEMENT AB There is increasing interest in using plant-derived extracts to promote growth and health in finfish species in recent years. Elucidating the effects of plant secondary metabolites on skeletal muscle growth signaling will contribute to an improved understanding of the effects of feeding carnivorous fish diets supplemented with plant extracts on fish somatic growth. Dietary intake of anthocyanins, a type of flavonoid widely distributed in plants, has long been associated with beneficial effects in both human and animal health; however, their effects in finfish are largely unknown. We conducted an experiment to test the effect of three doses (treatments A, B and C; 1x, 2.5x and 10x, respectively) of a mixture of three types of anthocyanidins (peonidin, cyanidin and pelargonidin chloride) on the expression of several genes in primary myogenic cells isolated from the skeletal muscle of rain-bow trout (Oncorhynchus mykiss) after 24 h of treatment. The genes of interest analyzed are involved inmyogenic programing (pax7, myoD and myogenin), Notch signaling (her6 and hey2) and antioxidant enzymes (sod1, cat and gpx1). Significantly greater expression of pax7 in cells under treatment B compared with the untreated cells was detected. Although no differences in expression of myogenic regulatory factors, myoD and myogenin between test groups or the control were detected, a trend toward significantly lower expression in all groups tested compared with the control group was observed. Moreover, significantly higher expression levels of her6 and hey2 in cells under treatments A and B compared with untreated cells were detected. Although no significant differences in the expression of cat and sod1, significantly greater expression in gpx1 in all treated groups compared with the control group was detected. Collectively, we demonstrated that anthocyanidins enhance the expression of gpx1 in primary myogenic cells, thereby contributing to skeletal muscle tissue defense against oxidative stress in finfish species. Further, anthocyanidins appear to delay myogenic differentiation in primary myogenic cells by up-regulating the expression of pax7 while decreasing myogenic regulatory factors in a Notch signaling-dependent interaction. Whether this effect results a reduced growth performance and/or an increase in feed conversion ratio in fish fed diets supplemented with plant extracts rich in anthocyanins or anthocyanidins needs further study, and the need to better define the potential effects of different polyphenol classes in myogenic differentiation on primary myogenic cells from carnivorous fish is warranted. Statement of relevance: The study contributes to increase our understanding regarding the effect of plant-derived secondary metabolites such as anthocyanidins on myogenic program and antioxidant enzyme defense in differentiating myogenic cells from carnivorous fish. We have demonstrated that anthocyanidins may delay the progress of the myogenic differentiation process and promote antioxidant defense expression in myogenic cells. (C) 2015 Elsevier B.V. All rights reserved. C1 [Villasante, Alejandro; Powell, Madison S.; Hardy, Ronald W.] Univ Idaho, Inst Aquaculture Res, Hagerman, ID 83332 USA. [Villasante, Alejandro; Wacyk, Jurij] Univ Chile, Fac Ciencias Agron, Dept Prod Anim, Casilla 1004, Santiago, Chile. [Moutou, Katerina] Univ Thessaly, Dept Biochem & Biotechnol, Larisa 41221, Greece. [Murdoch, Gordon K.] Univ Idaho, Dept Anim & Vet Sci, Moscow, ID 83844 USA. [Overturf, Ken] USDA ARS, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. RP Villasante, A (reprint author), Univ Idaho, Inst Aquaculture Res, Hagerman, ID 83332 USA.; Villasante, A (reprint author), Univ Chile, Fac Ciencias Agron, Dept Prod Anim, Casilla 1004, Santiago, Chile. EM alejandro.villasante@gmail.com RI Wacyk, Jurij/B-6544-2014 OI Wacyk, Jurij/0000-0002-9044-2755 NR 73 TC 2 Z9 2 U1 4 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD MAR 1 PY 2016 VL 454 BP 81 EP 89 DI 10.1016/j.aquaculture.2015.12.007 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DB7JC UT WOS:000368690000010 ER PT J AU Cherubin, MR Franco, ALC Cerri, CEP Karlen, DL Pavinato, PS Rodrigues, M Davies, CA Cerri, CC AF Cherubin, Mauricio R. Franco, Andre L. C. Cerri, Carlos E. P. Karlen, Douglas L. Pavinato, Paulo S. Rodrigues, Marcos Davies, Christian A. Cerri, Carlos C. TI Phosphorus pools responses to land-use change for sugarcane expansion in weathered Brazilian soils SO GEODERMA LA English DT Article DE Sugarcane production; Environmental sustainability; Phosphorus fractions; Soil quality indicators; Soil macrofauna ID TROPICAL SOILS; ORGANIC-MATTER; CARBON STOCKS; FRACTIONS; PASTURE; NITROGEN; IMPACTS; QUALITY; AVAILABILITY; ECOSYSTEMS AB Without proper management, land-use change (LUC) associated with producing sugarcane (Saccharum officinarum) for bioenergy in Brazil can lead to soil degradation and have negative implications on ecosystem functions. Phosphorus (P) depletion is one of the most frequent causes of land degradation in tropical environments, and as such, soil P pools have been identified as potential indicators of negative environmental impacts. We quantified soil P dynamics for the most common LUC sequence in sugarcane expansion areas [i.e., native vegetation (NV) to pasture (PA) to sugarcane (SC)], to determine if and how these changes could be used to evaluate environmental impacts of LUC in weathered Brazilian soils. Soil samples were collected from three areas in central-southern Brazil, representing the primary sugarcane-producing region of the world. Soil P fractionation was performed, and P stocks were calculated for the surface 30 cm. Soil chemical attributes and macrofauna data were correlated with P pools. Long-term conversions from natural ecosystems (Cerrado and Atlantic forest biome) to extensive PA decreased total P stocks by 31.2% indicating progressive soil degradation in these areas. In contrast, the LUC from PA to SC increased total P stocks by 35.6%; nevertheless, fertilization management altered the soil P-cycle, causing P accumulation in less plant-available forms. Available P increases to support adequate SC growth are still needed. Applying P using organic residues increased labile organic P and may be a complementary strategy for increasing nutrient supplies in SC fields. Phosphorus availability showed significant positive correlations with other soil chemical properties and clay content, while the functional diversity of soil macrofauna was strongly correlated with labile and biological P. We conclude that P pools can be useful indicators for assessing LUC modifications on soil quality in the tropics, and recommend they be used to assess land degradation and environmental sustainability within SC expansion areas in Brazil. (C) 2015 Elsevier B.V. All rights reserved. C1 [Cherubin, Mauricio R.; Cerri, Carlos E. P.; Pavinato, Paulo S.; Rodrigues, Marcos] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, BR-13418900 Piracicaba, SP, Brazil. [Franco, Andre L. C.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Karlen, Douglas L.] USDA, Agr Res Serv, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Davies, Christian A.] Shell Technol Ctr Houston, Houston, TX 77082 USA. [Cerri, Carlos C.] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP, Brazil. RP Cherubin, MR (reprint author), Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, 11 Padua Dias Ave, BR-13418900 Piracicaba, SP, Brazil. EM cherubin@usp.br RI Rodrigues, Marcos/H-7594-2016; Cerri, Carlos Eduardo/C-5039-2012; Pavinato, Paulo /B-9513-2012; OI Rodrigues, Marcos/0000-0003-3214-8723; Pavinato, Paulo /0000-0003-3244-2666; Franco, Andre/0000-0003-3294-6848 FU Sao Paulo Research Foundation (FAPESP) [2013/17581-6, 2014/16612-8, 2012/22510-8]; Raizen FX M.R.C. and A.L.C.F. thank Sao Paulo Research Foundation (FAPESP) for research grants (Processes 2013/17581-6, 2014/16612-8 and 2012/22510-8). The authors are grateful to the Raizen for all the support received and access provided for this research. NR 76 TC 0 Z9 0 U1 17 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD MAR 1 PY 2016 VL 265 BP 27 EP 38 DI 10.1016/j.geoderma.2015.11.017 PG 12 WC Soil Science SC Agriculture GA DB8DC UT WOS:000368746200005 ER PT J AU Chirici, G McRoberts, RE Fattorini, L Mura, M Marchetti, M AF Chirici, Gherardo McRoberts, Ronald E. Fattorini, Lorenzo Mura, Matteo Marchetti, Marco TI Comparing echo-based and canopy height model-based metrics for enhancing estimation of forest aboveground biomass in a model-assisted framework SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Airborne laser scanning metrics; Forest biomass; Model-assisted estimator; k-Nearest Neighbors; Tessellation stratified sampling ID LASER SCANNER DATA; GROWING STOCK VOLUME; NEAREST NEIGHBORS TECHNIQUE; AIRBORNE LASER; HEDMARK COUNTY; AUXILIARY INFORMATION; STANDING VOLUME; LIDAR; FIELD; NORWAY AB Among the forestry-related applications for which airborne laser scanning (ALS) data have been shown to be beneficial, forest inventory has been investigated as much if not more than other applications. Metrics extracted from ALS data for spatial units such as plots and grid cells are typically of two forms: echo-based metrics derived directly from the three-dimensional distribution of the point cloud data and metrics derived from a canopy height model (CHM). For both cases, a large number of metrics can be calculated and used to construct parametric and non-parametric models to predict forest variables. We compared model-assisted estimates of total forest aboveground biomass (AGB) obtained using echo-based and CHM-based height metrics with two prediction methods: (i) a parametric linear model, and (ii) the non parametric k-Nearest Neighbors (k-NN) technique. Model-assisted (MA) estimators were used with sample data obtained using a two-phase, tessellation stratified sampling (TSS) framework to estimate population parameters. The study was conducted in Molise Region in central Italy. For the four combinations of metrics and prediction techniques, estimates of total biomass were similar, in the range 1.96-2.1 million t, with standard error estimates that were also similar, in the range 0.20-0.21 t. Thus, the CHM-based metrics produced AGB estimates that were similar to and as accurate as those for the echo based metrics, regardless of whether the parametric or the non-parametric prediction method was used. Additionally, the proposed MA estimator was more accurate than the estimator that did not use auxiliary data. (C) 2015 Elsevier Inc. All rights reserved. C1 [Chirici, Gherardo] Univ Florence, Dept Agr Food & Forestry Syst, I-50121 Florence, Italy. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN USA. [Fattorini, Lorenzo] Univ Siena, Dept Econ & Stat, I-53100 Siena, Italy. [Mura, Matteo; Marchetti, Marco] Univ Molise, Dept Biosci & Terr, Contrada Fonte Lappone Snc, I-86090 Pesche, IS, Italy. RP Mura, M (reprint author), Univ Molise, Dept Biosci & Terr, Contrada Fonte Lappone Snc, I-86090 Pesche, IS, Italy. EM mur.teo@gmail.com FU Italian Ministry of University and Research [2012EWEY2S] FX This work was partially supported by the Italian Ministry of University and Research in the framework of the project: "Development of innovative methods for forest ecosystems monitoring based on remote sensing" (PRIN2012, grant 2012EWEY2S, project coordinator: G. Chirici). NR 52 TC 9 Z9 11 U1 4 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 1 PY 2016 VL 174 BP 1 EP 9 DI 10.1016/j.rse.2015.11.010 PG 9 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DB8DI UT WOS:000368746800001 ER PT J AU Anderson, MC Zolin, CA Sentelhas, PC Hain, CR Semmens, K Yilmaz, MT Gao, F Otkin, JA Tetrault, R AF Anderson, Martha C. Zolin, Cornelio A. Sentelhas, Paulo C. Hain, Christopher R. Semmens, Kathryn Yilmaz, M. Tugrul Gao, Feng Otkin, Jason A. Tetrault, Robert TI The Evaporative Stress Index as an indicator of agricultural drought in Brazil: An assessment based on crop yield impacts SO REMOTE SENSING OF ENVIRONMENT LA English DT Article ID LIGHT-USE-EFFICIENCY; REMOTE-SENSING DATA; TIME-SERIES; WATER PRODUCTIVITY; SATELLITE IMAGERY; VEGETATION INDEX; UNITED-STATES; GRAIN-YIELD; MODEL; CLIMATE AB To effectively meet growing food demands, the global agronomic community will require a better understanding of factors that are currently limiting crop yields and where production can be viably expanded with minimal environmental consequences. Remote sensing can inform these analyses, providing valuable spatiotemporal information about yield-limiting moisture conditions and crop response under current climate conditions. In this paper we study correlations for the period 2003-2013 between yield estimates for major crops grown in Brazil and the Evaporative Stress Index (ESI) - an indicator of agricultural drought that describes anomalies in the actual/reference evapotranspiration (ET) ratio, retrieved using remotely sensed inputs of land surface temperature (LST) and leaf area index (LAI). The strength and timing of peak ESI-yield correlations are compared with results using remotely sensed anomalies in water supply (rainfall from the Tropical Rainfall Mapping Mission; TRMM) and biomass accumulation (LAI from the Moderate Resolution Imaging Spectroradiometer; MODIS). Correlation patterns were generally similar between all indices, both spatially and temporally, with the strongest correlations found in the south and northeast where severe flash droughts have occurred over the past decade, and where yield variability was the highest. Peak correlations tended to occur during sensitive crop growth stages. At the state scale, the ESI provided higher yield correlations for most crops and regions in comparison with TRMM and LAI anomalies. Using finer scale yield estimates reported at the municipality level, ESI correlations with soybean yields peaked higher and earlier by 10 to 25 days in comparison to TRMM and LAI, respectively. In most states, TRMM peak correlations were marginally higher on average with municipality-level annual corn yield estimates, although these estimates do not distinguish between primary and late season harvests. A notable exception occurred in the northeastern state of Bahia, where the ESI better captured effects of rapid cycling of moisture conditions on corn yields during a series of flash drought events. The results demonstrate that for monitoring agricultural drought in Brazil, value is added by combining LAI with LST indicators within a physically based model of crop water use. Published by Elsevier Inc. C1 [Anderson, Martha C.; Gao, Feng] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Zolin, Cornelio A.] Embrapa Agrosilvopastoral, POB 343, BR-78550970 Sinop, MT, Brazil. [Sentelhas, Paulo C.] Univ Sao Paulo, ESALQ, Dept Biosyst Engn, Piracicaba, SP, Brazil. [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Semmens, Kathryn] Nat Nurture Ctr, Easton, PA USA. [Yilmaz, M. Tugrul] Middle E Tech Univ, Dept Civil Engn, Div Water Resources, TR-06531 Ankara, Turkey. [Otkin, Jason A.] Univ Wisconsin, Cooperat Inst Meteorol Satellite Studies, Madison, WI USA. [Tetrault, Robert] Foreign Agr Serv, USDA, Washington, DC USA. RP Anderson, MC (reprint author), 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM martha.anderson@ars.usda.gov RI Otkin, Jason/D-1737-2012; Sentelhas, Paulo/B-7849-2012; Anderson, Martha/C-1720-2015 OI Otkin, Jason/0000-0003-4034-7845; Anderson, Martha/0000-0003-0748-5525 FU Embrapa Visiting Scientist Program; Labex US, an international scientific cooperation program - Brazilian Agricultural Research Corporation - Embrapa, [10200.10/0215-9]; Agricultural Research Service - ARS FX This research was supported by funding from the Embrapa Visiting Scientist Program and from Labex US, an international scientific cooperation program sponsored by the Brazilian Agricultural Research Corporation - Embrapa, through contract 10200.10/0215-9 with the Agricultural Research Service - ARS. The authors would like to thank two anonymous reviewers for their time and constructive comments, which helped us to improve the manuscript. NR 85 TC 6 Z9 6 U1 15 U2 55 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 1 PY 2016 VL 174 BP 82 EP 99 DI 10.1016/j.rse.2015.11.034 PG 18 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DB8DI UT WOS:000368746800007 ER PT J AU Barrett, F McRoberts, RE Tomppo, E Cienciala, E Waser, LT AF Barrett, Frank McRoberts, Ronald E. Tomppo, Erkki Cienciala, Emil Waser, Lars T. TI A questionnaire-based review of the operational use of remotely sensed data by national forest inventories SO REMOTE SENSING OF ENVIRONMENT LA English DT Review DE COST action FP1001; Case studies; Satellite imagery; Spaceborne data; Aerial photography ID CONTERMINOUS UNITED-STATES; GROWING STOCK VOLUME; LAND-COVER DATABASE; STRATIFIED ESTIMATION; SATELLITE IMAGERY; BOREAL FOREST; X-BAND; AREA; VARIABLES; ACCURACY AB We report on the operational use of remotely sensed data by national forest inventory (NFI) programmes in 45 countries representing approximately 65% of the world's forest area. The analysis is based on responses to a questionnaire prepared under the auspices of COST Action FP1001 "Improving Data and Information on the Potential Supply of Wood Resources: A European Approach from Multisource National Forest Inventories (USEWOOD)". Responses were received from NH remote sensing experts from both European and non-European countries. Three major conclusions were drawn from the study: (1) remote sensing now plays an essential role in many NH programmes and provides data that can be used to enhance estimates for the most meaningful and commonly reported forest resource parameters; (2) a wide spectrum of remote sensing methods are currently used by NH teams; and (3) although substantial effort and attention has been focused on the use of aerial photography and spaceborne sensor data for mapping and enhancing estimation, integration of uncertainly estimation requires additional attention. The operational use of remotely sensed data by NFI programmes is illustrated for three case studies: a case study for Switzerland focuses on digital aerial photography, a case study for Finland focuses on spaceborne sensor data for small area estimation, and a case study for the USA focuses on spaceborne sensor data for increasing the precision of large area estimates. Although use of remotely sensed data by NFI programmes may remain region-specific and some approaches are not readily transferable, generally applicable good practice guidelines were formulated on the basis of the questionnaire responses and the case studies. These guidelines are intended to promote better use of limited financial resources and to increase the accuracy and precision of NH estimates. Published by Elsevier Inc. C1 [Barrett, Frank] Irish Forest Serv, Dept Agr Food & Marine, Wexford, Ireland. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. [Tomppo, Erkki] Nat Resources Inst Finland Luke, Vantaa, Finland. [Cienciala, Emil] IFER, Jilove, Czech Republic. [Waser, Lars T.] Swiss Fed Inst Forest Snow & Landscape Res, WSL, Birmensdorf, Switzerland. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us RI Cienciala, Emil/F-1873-2011 OI Cienciala, Emil/0000-0002-1254-4254 FU COST (European Cooperation in Scientific and Technical Research) FX This article is a product of Working Group 2 of COST Action FP1001 (USEWOOD). COST (European Cooperation in Scientific and Technical Research), financially supported the meetings and Short Term Scientific Missions making this work possible. We thank the respondents from 45 countries who participated in our questionnaire survey: Alberto Sandoval Uribe, Jacques Rondeux, Marc Wagner, Celina Montenegro, Conceicao Moreno, Paul Lane, Claire Howell, Georgios A. Georgiou, Jose Uva, Marko Kovac, Novica Tmusic, Yasna Rojas Ponce, Emine Atas Marek Jablonski, Nickola Stoyanov, Nicholas Py, Azra Cabaravdic, Zsolt Magyar, Sung-Ho Kim, Graham Stinson, Annemarie Bastrup-Birk, Vivian K. Johannsen, Joberto Veloso de Freitas, Juris Zarins, Mark Lawrence, Xiangdong Lei, Bjorn Traustason, Daniel Nitu, Lars Waser, Veiko Adermann, Wim P. Daamen, Christoph Bauerhansl, Tomas Bucha, Mats Nilsson, Yasumasa Hirata, Gerald Kandler, Heino Polley, Erkki Tomppo, Ronald E. McRoberts, Radim Adolt, Svein Solberg, Ioannis Meliadis, Julien Philippart, Patrizia Gasparini, Santiago Saura, Gintautas Mozgeris, Tatiana Chernenkova, Christian Ginzler. NR 75 TC 5 Z9 5 U1 4 U2 13 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 1 PY 2016 VL 174 BP 279 EP 289 DI 10.1016/j.rse.2015.08.029 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DB8DI UT WOS:000368746800022 ER PT J AU Lienard, J Vogs, A Gatziolis, D Strigul, N AF Lienard, Jean Vogs, Andre Gatziolis, Demetrios Strigul, Nikolay TI Embedded, real-time UAV control for improved, image-based 3D scene reconstruction SO MEASUREMENT LA English DT Article DE 3D reconstruction; Aerial robotics; Computer vision; Robotics in agriculture and forestry; Real-time photogrammetry ID MICRO AERIAL VEHICLE; SYSTEMS AB Unmanned Aerial Vehicles (UAVs) are already broadly employed for 3D modeling of large objects such as trees and monuments via photogrammetry. The usual workflow includes two distinct steps: image acquisition with UAV and computationally demanding post-flight image processing. Insufficient feature overlaps across images is a common shortcoming in post-flight image processing resulting in the failure of 3D reconstruction. Here we propose a real-time control system that overcomes this limitation by targeting specific spatial locations for image acquisition thereby providing sufficient feature overlap. We initially benchmark several implementations of the Scale-Invariant Feature Transform (SIFT) feature identification algorithm to determine whether they allow real-time execution on the low-cost processing hardware embedded on the UAV. We then experimentally test our UAV platform in virtual and real-life environments. The presented architecture consistently decreases failures and improves the overall quality of 3D reconstructions. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Lienard, Jean; Strigul, Nikolay] Washington State Univ, Dept Math & Stat, Vancouver, WA 98686 USA. [Vogs, Andre] Intel Inc, Hillsboro, OR 97124 USA. [Gatziolis, Demetrios] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97204 USA. RP Strigul, N (reprint author), Washington State Univ, Dept Math & Stat, Vancouver, WA 98686 USA. EM nick.strigul@wsu.edu RI Strigul, Nick/C-3987-2016 NR 32 TC 3 Z9 3 U1 10 U2 59 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0263-2241 EI 1873-412X J9 MEASUREMENT JI Measurement PD MAR PY 2016 VL 81 BP 264 EP 269 DI 10.1016/j.measurement.2015.12.014 PG 6 WC Engineering, Multidisciplinary; Instruments & Instrumentation SC Engineering; Instruments & Instrumentation GA DB1IP UT WOS:000368262100022 ER PT J AU Romanazzi, G Smilanick, JL Feliziani, E Droby, S AF Romanazzi, Gianfranco Smilanick, Joseph L. Feliziani, Erica Droby, Samir TI Integrated management of postharvest gray mold on fruit crops SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Biological control; Botrytis cinerea; Cold storage; Natural antimicrobials; Postharvest decay ID BIOLOGICAL-CONTROL AGENTS; BOTRYTIS-CINEREA; TABLE GRAPES; ESSENTIAL OILS; COLD-STORAGE; PENICILLIUM-EXPANSUM; SODIUM-BICARBONATE; NATURAL-PRODUCTS; OZONE EXPOSURE; DECAY CONTROL AB Gray mold, incited by Botrytis cinerea, causes major postharvest losses in a wide range of crops. Some infections that occur in the field remain quiescent during the growing season and develop after harvest. The pathogen is also capable of infecting plant tissues through surface injuries inflicted during harvesting and subsequent handling; these develop during storage, even at 0 degrees C, and spread among products by aerial mycelial growth and conidia. The postharvest decay by this pathogen is controlled by a combination of preharvest and postharvest practices. To minimize postharvest gray mold, control programs rely mainly on applications of fungicides. However, mounting concerns of consumers and regulatory authorities about risks associated with chemical residues in food have led to imposition of strict regulations, the banning of use of certain chemical groups, and preferences by wholesaler, retailers and consumers to avoid chemically treated produce. These developments have driven the search for alternative management strategies that are effective and not reliant on conventional fungicide applications. In this review, conventional and alternative control strategies are discussed including their advantages and disadvantages. They include the use of conventional fungicides, biocontrol agents, physical treatments, natural antimicrobials, and disinfecting agents. Based on examples to control gray mold on specific crops, it is concluded that an integrated management program where adoption of a holistic approach is the key for meeting the challenge of minimizing postharvest losses caused by B. cinerea. To optimize the efficacy of treatments, it is essential to understand their mechanism of action as much as possible. Information about direct and indirect effects of each approach on the pathogen is also presented. (C) 2015 Elsevier B.V. All rights reserved. C1 [Romanazzi, Gianfranco; Feliziani, Erica] Marche Polytech Univ, Dept Agr Food & Environm Sci, I-60131 Ancona, Italy. [Smilanick, Joseph L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Droby, Samir] Agr Res Org, Volcani Ctr, Dept Postharvest Sci ARO, IL-50250 Bet Dagan, Israel. RP Romanazzi, G (reprint author), Marche Polytech Univ, Dept Agr Food & Environm Sci, Via Brecce Blanche, I-60131 Ancona, Italy. EM g.romanazzi@univpm.it; joe.smilanick@gmail.com; samird@volcani.agri.gov.il RI Romanazzi, Gianfranco/C-8981-2012; OI Romanazzi, Gianfranco/0000-0003-0390-4008 FU EUBerry Project [EU] [265942] FX This work was supported by EUBerry Project [EU FP7 KBBE 2010-4, Grant Agreement No. 265942]. Thanks are expressed to Dr. Antonio Romito and Agriproject team, and to Dr Gianluca Savini (Sant'Orsola) for sharing information during surveys. NR 65 TC 9 Z9 11 U1 12 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2016 VL 113 BP 69 EP 76 DI 10.1016/j.postharvbio.2015.11.003 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DB0RU UT WOS:000368216000010 ER PT J AU Hou, LX Johnson, JA Wang, SJ AF Hou, Lixia Johnson, Judy A. Wang, Shaojin TI Radio frequency heating for postharvest control of pests in agricultural products: A review SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Review DE Radio frequency; Agricultural products; Postharvest; Disinfestations; Heating uniformity ID THERMAL-DEATH KINETICS; CONTROL CODLING MOTH; IN-SHELL WALNUTS; DEPENDENT DIELECTRIC-PROPERTIES; TOP ELECTRODE VOLTAGE; STORED-GRAIN INSECTS; HOT-WATER TREATMENTS; BING SWEET CHERRIES; COMPUTER-SIMULATION; DISINFESTATION TREATMENTS AB Radio frequency (RF) treatment is one of the most promising physical disinfestation methods in agricultural products due to rapid heating, deep penetration depth, and leaving no chemical residues. This paper focuses on reviewing uses of RF energy for disinfestation of agricultural products. It provides a brief introduction on the basic principle of RF heating technology, analyzes the differential heating of pests in host products at RF range, and discusses the factors influencing the RF heating uniformity and the possible methods to improve heating uniformity by computer simulations. This paper presents a comprehensive review of recent progresses in developing RF treatment protocols for disinfesting fresh fruits and dry products, and recommendations for future research to effectively achieve the required RF heating uniformity and bridge the gap between laboratory research and industrial applications. (C) 2015 Elsevier B.V. All rights reserved. C1 [Hou, Lixia; Wang, Shaojin] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Johnson, Judy A.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Wang, Shaojin] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Wang, SJ (reprint author), Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM shaojinwang@nwsuaf.edu.cn FU Open Fund for Key Laboratory of Physical Processing of Agricultural Product in Jiangsu Province [JAPP2013-2]; General Program of National Natural Science Foundation of China [31371853]; Shaanxi Agricultural Science and Technology Innovation and Research Project [2013K01-50] FX This research was conducted in the College of Mechanical and Electronic Engineering, Northwest A&F University, and supported by research grants from Open Fund for Key Laboratory of Physical Processing of Agricultural Product in Jiangsu Province (JAPP2013-2), General Program of National Natural Science Foundation of China (31371853) and Shaanxi Agricultural Science and Technology Innovation and Research Project (2013K01-50). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 100 TC 6 Z9 6 U1 13 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2016 VL 113 BP 106 EP 118 DI 10.1016/j.postharvbio.2015.11.011 PG 13 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DB0RU UT WOS:000368216000014 ER PT J AU Argenta, LC Mattheis, JP Fan, XT Amarante, CVT AF Argenta, Luiz C. Mattheis, James P. Fan, Xuetong Amarante, Cassandro V. T. TI Managing 'Bartlett' pear fruit ripening with 1-methylcyclopropene reapplication during cold storage SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Pyrus communis L.; Ethylene inhibition; Quality; Firmness; Physiological disorders ID GOLDEN DELICIOUS APPLE; COMMUNIS L. FRUIT; ETHYLENE BIOSYNTHESIS; 1-MCP TREATMENTS; RECEPTOR LEVEL; DANJOU PEARS; SHELF-LIFE; PERCEPTION; EXPRESSION; QUALITY AB Repeated low-dose 1-MCP-applications were evaluated during cold storage of 'Bartlett' pear fruit to overcome long-term ripening inhibition of a high dose 1-MCP treatment at harvest. Fruit were exposed to 1-MCP at 0, 0.42, 4.2 or 42 mu mol m(-3) at harvest in year one, and to 0, 0.42 or 42 mu mol m(-3) in year two, and then stored in air at 0.5 degrees C. In year two, fruit exposed to 1-MCP at 0.42 mu mol m(-3) at harvest were retreated during cold storage once (after 38 days) or twice (after 38 and 68 days), when ethylene production in samples removed from cold storage exceeded 0.014 eta mol kg(-1) s(-1) within 7 days at 20 degrees C. 1-MCP was re-applied once at 0.42 or 4.2 mu mol m(-3) or twice at 0.42 or 4.2 then 42 mu mol m(-3). In year one, fruit treatment at harvest with 4.2 or 42 mu mol m(-3) 1-MCP provided similar ripening delay during 120 days in storage followed by 7 days at 20 degrees C, while fruit treated with 0.42 mu mol m(-3) 1-MCP was not different from the control. In year two, fruit treated at harvest with 0.42 mu mol m(-3) 1-MCP and retreated with 0.42 mu mol m(-3) (when ethylene production was already high) did not delay subsequent fruit ripening. Fruit treated at harvest with 42 mu mol m(-3) 1-MCP or with 0.42 mu mol m(-3) at harvest and then +4.2 +42, had similar peel yellow color, TA and SSC, but higher firmness after 180 days storage, compared to control fruit after 60 days storage. After 180 days storage, the severity of superficial scald, senescent scald and core browning on fruit treated only at harvest with 42 mu mol m(-3) were lower than on control fruit and similar to on fruit treated with 0.42 mu mol m(-3) at harvest and then retreated with +4.2 +42. Therefore, a low dose application of 1-MCP at harvest followed by reapplication with higher doses based on fruit ethylene production capacity after removal from cold storage can extend 'Bartlett' pear storage life while allowing ripening to occur after mid-term storage. The efficiency of this procedure will depend on timing and 1-MCP reapplication concentration. Published by Elsevier B.V. C1 [Argenta, Luiz C.] EPAGRI, Estacao Expt, BR-89500000 Cacador, SC, Brazil. [Mattheis, James P.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Fan, Xuetong] ARS, USDA, ERRC, Wyndmoor, PA 19038 USA. [Amarante, Cassandro V. T.] UDESC, Ctr Ciencias Agrovet, BR-88520000 Lages, SC, Brazil. RP Mattheis, JP (reprint author), ARS, USDA, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM james.mattheis@ars.usda.gov FU Northwest Pear Committee FX The authors thank David Buchanan and Janie Countryman for excellent technical assistance. Partial funding for this work was received from the Northwest Pear Committee. NR 40 TC 1 Z9 1 U1 6 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAR PY 2016 VL 113 BP 125 EP 130 DI 10.1016/j.postharvbio.2015.11.009 PG 6 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DB0RU UT WOS:000368216000016 ER PT J AU Gruber, A Su, CH Zwieback, S Crowd, W Dorigo, W Wagner, W AF Gruber, A. Su, C-H. Zwieback, S. Crowd, W. Dorigo, W. Wagner, W. TI Recent advances in (soil moisture) triple collocation analysis SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Soil moisture; Error characterization; Validation; Triple collocation ID ERROR CHARACTERIZATION; TEMPORAL STABILITY; RETRIEVALS; PRODUCTS AB To date, triple collocation (TC) analysis is one of the most important methods for the global-scale evaluation of remotely sensed soil moisture data sets. In this study we review existing implementations of soil moisture TC analysis as well as investigations of the assumptions underlying the method. Different notations that are used to formulate the TC problem are shown to be mathematically identical. While many studies have investigated issues related to possible violations of the underlying assumptions, only few TC modifications have been proposed to mitigate the impact of these violations. Moreover, assumptions, which are often understood as a limitation that is unique to IC analysis are shown to be common also to other conventional performance metrics. Noteworthy advances in TC analysis have been made in the way error estimates are being presented by moving from the investigation of absolute error variance estimates to the investigation of signal-to-noise ratio (SNR) metrics. Here we review existing error presentations and propose the combined investigation of the SNR (expressed in logarithmic units), the unscaled error variances, and the soil moisture sensitivities of the data sets as an optimal strategy for the evaluation of remotely-sensed soil moisture data sets. (C) 2015 Elsevier B.V. All rights reserved. C1 [Gruber, A.; Dorigo, W.; Wagner, W.] Vienna Univ Technol, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. [Su, C-H.] Univ Melbourne, Dept Infrastruct Engn, Melbourne, Vic 3010, Australia. [Zwieback, S.] ETH, Inst Environm Engn, Zurich, Switzerland. [Crowd, W.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Dorigo, W.] Univ Ghent, Lab Hydrol & Water Management, B-9000 Ghent, Belgium. RP Gruber, A (reprint author), Vienna Univ Technol, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. EM alexander.gruber@geo.tuwien.ac.at FU ESA's Climate Change Initiative (CCI) for soil moisture [4000104814/11/I-NB]; eartH2Observe project (European Union's Seventh Framework Programme) [603608] FX We thank Rolf Reichle and an anonymous reviewer for their valuable comments which significantly improved the quality of this paper. This study was carried out within ESA's Climate Change Initiative (CCI) for soil moisture (Contract No. 4000104814/11/I-NB) and the eartH2Observe project (European Union's Seventh Framework Programme, Grant Agreement No. 603608). NR 39 TC 15 Z9 15 U1 9 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD MAR PY 2016 VL 45 SI SI BP 200 EP 211 DI 10.1016/j.jag.2015.09.002 PN B PG 12 WC Remote Sensing SC Remote Sensing GA DA4LP UT WOS:000367771700010 ER PT J AU Dundar, T Wang, XP As, N Avci, E AF Dundar, Turker Wang, Xiping As, Nusret Avci, Erkan TI Potential of ultrasonic pulse velocity for evaluating the dimensional stability of oak and chestnut wood SO ULTRASONICS LA English DT Article DE Ultrasonic velocity; Dimensional stability; Shrinkage; Specific gravity; Wood ID MOISTURE-CONTENT AB The objective of this study was to examine the potential of ultrasonic velocity as a rapid and nondestructive method to predict the dimensional stability of oak (Quercus petraea (Mattuschka) Lieblein) and chestnut (Castanea sativa Mill.) that are commonly used in flooring industry. Ultrasonic velocity, specific gravity, and radial, tangential and volumetric shrinkages were measured on seventy-four 20 x 20 x 30-mm(3) specimens obtained from freshly cut oak and chestnut stems. The ultrasonic velocities of the specimens decreased with increasing moisture content (MC). We found that specific gravity was not a good predictor of the transverse shrinkages as indicated by relatively weak correlations. Ultrasonic velocity, on the other hand, was found to be a significant predictor of the transverse shrinkages for both oak and chestnut. The best results for prediction of shrinkages of oak and chestnut were obtained when the ultrasonic velocity and specific gravity were used together. The multiple regression models we developed in this study explained 77% of volumetric shrinkages in oak and 72% of volumetric shrinkages in chestnut. It is concluded that ultrasonic velocity coupled with specific gravity can be employed as predicting parameters to evaluate the dimensional stability of oak and chestnut wood during manufacturing process. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dundar, Turker; As, Nusret] Istanbul Univ, Fac Forestry, TR-34473 Istanbul, Turkey. [Wang, Xiping] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Avci, Erkan] Mugla Sitki Kocman Univ, Fac Technol, TR-48000 Kotekli, Mugla, Turkey. RP Dundar, T (reprint author), Istanbul Univ, Fac Forestry, TR-34473 Istanbul, Turkey. EM dundar@istanbul.edu.tr; xwang@fs.fed.us; nusretas@istanbul.edu.tr; erkanavci@mu.edu.tr NR 23 TC 0 Z9 0 U1 3 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0041-624X EI 1874-9968 J9 ULTRASONICS JI Ultrasonics PD MAR PY 2016 VL 66 BP 86 EP 90 DI 10.1016/j.ultras.2015.11.007 PG 5 WC Acoustics; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Radiology, Nuclear Medicine & Medical Imaging GA DA4TY UT WOS:000367795100018 PM 26678790 ER PT J AU Lewandowski, TE Forrester, JA Mladenoff, DJ D'Amato, AW Palik, BJ AF Lewandowski, Tera E. Forrester, Jodi A. Mladenoff, David J. D'Amato, Anthony W. Palik, Brian J. TI Response of the soil microbial community and soil nutrient bioavailability to biomass harvesting and reserve tree retention in northern Minnesota aspen-dominated forests SO APPLIED SOIL ECOLOGY LA English DT Article DE Populus tremuloides; Reserve tree patches; Biomass harvest; Soil microbial community; Soil nutrient availability; Slash retention ID FATTY-ACID PROFILES; SUBSURFACE BACTERIA; ORGANIC-MATTER; BOREAL FORESTS; CLEAR-CUT; MANAGEMENT; STANDS; NITROGEN; MINERALIZATION; VEGETATION AB Intensive forest biomass harvesting, or the removal of harvesting slash (woody debris from tree branches and tops) for use as biofuel, has the potential to negatively affect the soil microbial community (SMC) due to loss of carbon and nutrient inputs from the slash, alteration of the soil microclimate, and increased nutrient leaching. These effects could result in lowered forest productivity and threaten the long-term sustainability of forest management. Retaining organic material post-harvest, including greater amounts of harvesting slash and live trees, within harvested areas may ameliorate some negative effects of biomass harvesting on soil processes. We evaluated the effects of biomass harvests with reserve tree and slash retention on the SMC and soil nutrient bioavailability (assessed using plant-root simulator probes) in trembling aspen (Populus tremuloides Michx.) forests in northern Minnesota during the spring and summer, 1-3 years after harvest. Variable biomass removal levels tested include complete removal (whole tree harvest of boles and branches), complete slash retention (bole only harvest), and 20% slash retention (amount suggested by regional biomass harvesting guidelines). Compared to the unharvested control, biomass harvests had no effect on the multivariate SMC composition or microbial biomass, but did result in a 1-4% increase in arbuscular mycorrhizal fungal abundance and reduced bacterial stress two and three years after harvest. Additionally, biomass harvesting increased NH4 bioavailability during year one, and reduced NO3 bioavailability during year two when compared to unharvested controls. Among the three biomass harvests with differing levels of slash removal there were few differences in overall SMC composition, microbial biomass, and soil nutrients; however, the abundance of arbuscular mycorrhizal fungi, gram positive and actinomycete bacteria were significantly higher in harvested treatments with more slash retained. These results are specific to single rotation biomass harvesting in aspen stands due to the unique relationships between plants and their associated SMCs, and may not be directly applicable to forest biomass harvesting of other commercial forest tree species, or multiple rotations. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lewandowski, Tera E.; Forrester, Jodi A.; Mladenoff, David J.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Palik, Brian J.] US Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. RP Lewandowski, TE (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM tgalante@wisc.edu FU USDA-BRDI [2009-10006-05948]; Minnesota Forest Resources Council; Minnesota Environment and Natural Resources Trust Fund; USDA Forest Service, Northern Research Station; STAR Fellowship Assistance Agreement [F13B30497] FX This project was supported by a USDA-BRDI Grant 2009-10006-05948, the Minnesota Forest Resources Council, Minnesota Environment and Natural Resources Trust Fund, and the USDA Forest Service, Northern Research Station. Many thanks to our field and lab crews for assistance with data collection; specifically Josh Kragthorpe and Alaina Berger for the coordination and collection of field data. Thank you to Dr. John Bradford and Dr. Randy Kolka at the USDA Forest Service, Northern Research Station for their contributions to the study design, ideas and discussion. Additionally, from the University of Wisconsin-Madison we would like to thank Dr. Harry Read for analyzing all lipid extracts on the gas chromatograph, Dr. Devin Wixon for the creation of a lipid processing program, and Nicholas Keuler for his statistical assistance. Special thanks to the St. Louis County Land Department and Minnesota Department of Natural Resources for allowing access to field sites and support with harvesting logistics. This paper was developed under STAR Fellowship Assistance Agreement no. [F13B30497] awarded by the U.S. Environmental Protection Agency (EPA). It has not been formally reviewed by EPA. The views expressed in this paper are solely those of Tera Lewandowski, and EPA does not endorse any products or commercial services mentioned in this paper. NR 64 TC 1 Z9 1 U1 12 U2 68 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD MAR PY 2016 VL 99 BP 13 EP 20 DI 10.1016/j.apsoil.2015.11.001 PG 8 WC Soil Science SC Agriculture GA CZ6UG UT WOS:000367235900002 ER PT J AU Wang, BN Huang, QY Venkitasamy, C Chai, HK Gao, H Cheng, N Cao, W Lv, XG Pan, ZL AF Wang, Bini Huang, Qingyuan Venkitasamy, Chandrasekar Chai, Hongkang Gao, Hui Cheng, Ni Cao, Wei Lv, Xingang Pan, Zhongli TI Changes in phenolic compounds and their antioxidant capacities in jujube (Ziziphus jujuba Miller) during three edible maturity stages SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Ziziphus; Insoluble-bound phenolic; Antioxidant activity; Maturity stage ID RIPENING STAGES; L. FRUIT; FLAVONOIDS; EXTRACTS; ACIDS; PLANTS; FIBER; PEEL AB This study investigated the changes in total phenolic content (TPC), total flavonoid content (TFC), individual phenolic compound content, DPPH radical scavenging activity and antioxidant capacity measured by FRAP assay of four phenolic fractions (free, esterifled, glycosided and insoluble-bound) from jujube during three edible maturity stages. The maturity stages of jujubes were established as white maturity (WM), half-red maturity (HM) and red maturity (RM). The free fraction in jujube at WM stage had the highest TPC, TFC, total phenolic acid contents, and antioxidant capacities. The phenolic contents and their activities greatly decreased with the increasing maturity stage. Caffeic acid was the most predominant in all detected phenolic compounds at WM stage, while rutin dominated at HM and RM stages. Even though most of phenolic compounds with antioxidant activity in jujube existed at the WM stage as the free form, the insoluble-bound fractions also contained a large number of phenolic compounds. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Wang, Bini; Huang, Qingyuan; Chai, Hongkang; Gao, Hui; Cheng, Ni; Cao, Wei; Lv, Xingang] Northwest Univ, Coll Chem Engn, Dept Food Sci & Engn, Xian 710069, Shaanxi, Peoples R China. [Wang, Bini; Venkitasamy, Chandrasekar; Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli] USDA ARS WRRC, Hlth Proc Foods Res Unit, Albany, CA 94710 USA. RP Wang, BN (reprint author), Northwest Univ, Coll Chem Engn, Dept Food Sci & Engn, Xian 710069, Shaanxi, Peoples R China. EM wangbini@nwu.edu.cn FU National Natural Science Foundation of China [31101325]; Science & Technology Project of Shaanxi Province [2012K02-06] FX This work is financially supported by the National Natural Science Foundation of China (31101325) and the Science & Technology Project of Shaanxi Province (2012K02-06). NR 30 TC 2 Z9 2 U1 11 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD MAR PY 2016 VL 66 BP 56 EP 62 DI 10.1016/j.lwt.2015.10.005 PG 7 WC Food Science & Technology SC Food Science & Technology GA CZ9JF UT WOS:000367413200009 ER PT J AU Singh, H Cantoria, MJ Malave, P Saputra, D Maleki, S AF Singh, Harmit Cantoria, Mary Jo Malave, Poonam Saputra, Denny Maleki, Soheila TI Standardization of RP-HPLC methods for the detection of the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 SO FOOD CHEMISTRY LA English DT Article DE Peanut; Allergen; HPLC; Ara h 1; Ara h 2; Ara h 3; Retention time ID FOOD ALLERGENS; PROTEINS; PURIFICATION; ARA-H-2; PHASE; ELISA AB Crude peanut extract (CPE) was analyzed for three major allergens (Ara h 1, h 2, and h 3) using a C12 and a C-18 column at two wavelengths (280 and 220 nm) and under different solvent conditions. HPLC profiles were compared for retention time, resolution, and peak heights. CPE samples were spiked with pure allergens to identify the peaks corresponding to allergens. The HPLC fractions of corresponding allergens were collected and freeze-dried in order to perform SDS-PAGE and immunoblotting tests. The best method was identified the one with a shorter retention time, better resolution, and greater peak height as compared with the other methods. In general, the peak heights were greater at 220 nm than at 280 nm. The major disadvantage of the C-12 column was the need for two sets of conditions to identify the allergens as compared to the C-18 column where all three allergens could be identified in one run. Published by Elsevier Ltd. C1 [Singh, Harmit; Saputra, Denny] Calif State Polytech Univ Pomona, Dept Human Nutr & Food Sci, Pomona, CA 91768 USA. [Cantoria, Mary Jo; Malave, Poonam] Calif State Univ Los Angeles, Los Angeles, CA 90032 USA. [Maleki, Soheila] USDA, So Reg Res Ctr, New Orleans, LA 70179 USA. RP Singh, H (reprint author), Calif State Polytech Univ Pomona, Dept Human Nutr & Food Sci, 3802 West Temple Ave, Pomona, CA 91768 USA. EM harmitsingh@cpp.edu FU USDA; Southern California Institute of Food Technologists (SCIFT) section FX We would like to thank Ms. Hsiaopo Cheng for her invaluable technical support. Financial assistance from USDA and Southern California Institute of Food Technologists (SCIFT) section is greatly appreciated. NR 24 TC 0 Z9 0 U1 13 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAR 1 PY 2016 VL 194 BP 383 EP 390 DI 10.1016/j.foodchem.2015.08.024 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CV4PL UT WOS:000364248900052 PM 26471570 ER PT J AU Lu, YJ Luthria, D AF Lu, Yingjian Luthria, Devanand TI Influence of gelatinization on the extraction of phenolic acids from wheat fractions SO FOOD CHEMISTRY LA English DT Article DE Gelatinization; LC-MS analysis; Total phenolic content and radical scavenging capacity; Refined and whole wheat fractions; Microwave and ultrasonic-assisted extractions ID PHYSICOCHEMICAL PROPERTIES; STARCH; DIGESTIBILITY AB The effect of gelatinization on the analysis of phenolic acids from wheat bran, whole-wheat, and refined flour samples was investigated using two extraction procedures, namely, ultrasonic (UAE) and microwave (MAE). The total phenolic acid (TPA) quantity in wheat bran (2711-2913 mu g/g) was significantly higher than the whole (664-715 mu g/g) and refined wheat (109-112 mu g/g) flour samples by both extraction methods as analyzed by liquid chromatography mass spectrometry. The recovery of phenolic acids from the spiked wheat bran sample was higher than from either the whole or refined wheat flour samples by both extraction procedures. The recovery of TPA (74-89%) from whole and refined wheat flours by MAE was significantly lower than that of UAE (90-98%). This difference was attributed to the gelatinization of starch present in the wheat flours caused by MAE. Gelatinization reduces the extractability of phenolic acids from wheat flour samples. Furthermore, both spectrometric assays (total phenolic content and radical scavenging capacities) showed similar trend as compared to LC-MS analyses. (C) 2015 Published by Elsevier Ltd. C1 [Lu, Yingjian; Luthria, Devanand] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Luthria, D (reprint author), ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Dave.Luthria@ars.usda.gov NR 19 TC 0 Z9 0 U1 4 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAR 1 PY 2016 VL 194 BP 1138 EP 1142 DI 10.1016/j.foodchem.2015.08.074 PG 5 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CV4PL UT WOS:000364248900146 PM 26471664 ER PT J AU Chang, HX Brown, PJ Lipka, AE Domier, LL Hartman, GL AF Chang, Hao-Xun Brown, Patrick J. Lipka, Alexander E. Domier, Leslie L. Hartman, Glen L. TI Genome-wide association and genomic prediction identifies associated loci and predicts the sensitivity of Tobacco ringspot virus in soybean plant introductions SO BMC GENOMICS LA English DT Article DE Genome association and prediction integrated tool (GAPIT); Genome-wide association studies (GWAS); Genomic prediction; Single nucleotide polymorphism (SNP); Soybean (Glycine max); Tobacco ringspot virus (TRSV) ID MAX L. MERR.; ARABIDOPSIS-THALIANA; COMPLEX TRAITS; BUD BLIGHT; RESISTANCE; KINASE; SELECTION; PROTEIN; ERECTA; GENE AB Background: Genome-wide association study (GWAS) is a useful tool for detecting and characterizing traits of interest including those associated with disease resistance in soybean. The availability of 50,000 single nucleotide polymorphism (SNP) markers (SoySNP50K iSelect BeadChip; www.soybase.org) on 19,652 soybean and wild soybean plant introductions (PIs) in the USDA Soybean Germplasm Collection allows for fast and robust identification of loci associated with a desired phenotype. By using a genome-wide marker set to predict phenotypic values, genomic prediction for phenotype-unknown but genotype-determined PIs has become possible. The goal of this study was to describe the genetic architecture associated with sensitivity to Tobacco ringspot virus (TRSV) infection in the USDA Soybean Germplasm Collection. Results: TRSV-induced disease sensitivities of the 697 soybean PIs were rated on a one to five scale with plants rated as one exhibiting mild symptoms and plants rated as five displaying terminal bud necrosis (i.e., bud blight). The GWAS identified a single locus on soybean chromosome 2 strongly associated with TRSV sensitivity. Cross-validation showed a correlation of 0.55 (P < 0.01) to TRSV sensitivity without including the most significant SNP marker from the GWAS as a covariate, which was a better estimation compared to the mean separation by using significant SNPs. The genomic estimated breeding values for the remaining 18,955 unscreened soybean PIs in the USDA Soybean Germplasm Collection were obtained using the GAPIT R package. To evaluate the prediction accuracy, an additional 55 soybean accessions were evaluated for sensitivity to TRSV, which resulted in a correlation of 0.67 (P < 0.01) between actual and predicted severities. Conclusion: A single locus responsible for TRSV sensitivity in soybean was identified on chromosome 2. Two leucine-rich repeat receptor-like kinase genes were located near the locus and may control sensitivity of soybean to TRSV infection. Furthermore, a comprehensive genomic prediction for TRSV sensitivity for all accessions in the USDA Soybean Germplasm Collection was completed. C1 [Chang, Hao-Xun; Brown, Patrick J.; Lipka, Alexander E.; Domier, Leslie L.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Domier, Leslie L.; Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, Natl Soybean Res Ctr, 1101 W Peabody Dr, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA.; Hartman, GL (reprint author), USDA ARS, Urbana, IL 61801 USA.; Hartman, GL (reprint author), Univ Illinois, Natl Soybean Res Ctr, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Chang, Hao-Xun/0000-0002-4667-7741 FU USDA Agricultural Research Service; University of Illinois FX This research was supported in part by the USDA Agricultural Research Service and the University of Illinois. The authors thank J.M. Lee and R. Warsaw for technical help, and Chong Yu and Hongxu Dong for their generous suggestions on genetics and rrBLUP. NR 39 TC 1 Z9 1 U1 4 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 29 PY 2016 VL 17 AR 153 DI 10.1186/s12864-016-2487-7 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DF8GB UT WOS:000371594300004 PM 26924079 ER PT J AU Letcher, BH Hocking, DJ O'Neil, K Whiteley, AR Nislow, KH O'Donnell, MJ AF Letcher, Benjamin H. Hocking, Daniel J. O'Neil, Kyle Whiteley, Andrew R. Nislow, Keith H. O'Donnell, Matthew J. TI A hierarchical model of daily stream temperature using air-water temperature synchronization, autocorrelation, and time lags SO PEERJ LA English DT Article DE Stream temperature; Ecology; Air temperature; Statistical model; Climate change ID GROWING DEGREE-DAY; RIVER TEMPERATURE; CLIMATE-CHANGE; MIXED MODELS; NETWORKS; PERSPECTIVE; DYNAMICS; ECOLOGY; INSECTS; TRENDS AB Water temperature is a primary driver of stream ecosystems and commonly forms the basis of stream classifications. Robust models of stream temperature are critical as the climate changes, but estimating daily stream temperature poses several important challenges. We developed a statistical model that accounts for many challenges that can make stream temperature estimation difficult. Our model identifies the yearly period when air and water temperature are synchronized, accommodates hysteresis, incorporates time lags, deals with missing data and autocorrelation and can include external drivers. Ina small stream network, the model performed well (RMSE 0.59 degrees C), identified a clear warming trend (0.63 degrees C decade(-1)) and a widening of the synchronized period (29 d decade(-1)). We also carefully evaluated how missing data influenced predictions. Missing data within a year had a small effect on performance (similar to 0.05% average drop in RMSE with 10% fewer days with data). Missing all data for a year decreased performance (similar to 0.6 degrees C jump in RMSE) but this decrease was moderated when data were available from other streams in the network. C1 [Letcher, Benjamin H.; Hocking, Daniel J.; O'Neil, Kyle; O'Donnell, Matthew J.] US Geol Survey, Leetown Sci Ctr, SO Conte Anadromous Fish Res Ctr, Turners Falls, MA 01376 USA. [Whiteley, Andrew R.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Nislow, Keith H.] Univ Massachusetts, USDA, Forest Serv, No Res Stn, Amherst, MA 01003 USA. RP Letcher, BH (reprint author), US Geol Survey, Leetown Sci Ctr, SO Conte Anadromous Fish Res Ctr, Turners Falls, MA 01376 USA. EM bletcher@usgs.gov OI Hocking, Daniel/0000-0003-1889-9184 FU USFWS North Atlantic Conservation Cooperative; USGS Northeastern Climate Science Center FX Funding was provided by the USFWS North Atlantic Conservation Cooperative and the USGS Northeastern Climate Science Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 68 TC 1 Z9 1 U1 4 U2 11 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD FEB 29 PY 2016 VL 4 AR e1727 DI 10.7717/peerj.1727 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF9DZ UT WOS:000371661900005 PM 26966662 ER PT J AU Frey, CF Regidor-Cerrillo, J Marreros, N Garcia-Lunar, P Gutierrez-Exposito, D Schares, G Dubey, JP Gentile, A Jacquiet, P Shkap, V Cortes, H Ortega-Mora, LM Alvarez-Garcia, G AF Frey, Caroline F. Regidor-Cerrillo, Javier Marreros, Nelson Garcia-Lunar, Paula Gutierrez-Exposito, Daniel Schares, Gereon Dubey, Jitender P. Gentile, Arcangelo Jacquiet, Philippe Shkap, Varda Cortes, Helder Ortega-Mora, Luis M. Alvarez-Garcia, Gema TI Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates SO PARASITES & VECTORS LA English DT Article DE Besnoitia besnoiti; Besnoitia tarandi; Lytic cycle; In vitro model; Isolates; Invasion; Proliferation ID NEOSPORA-CANINUM; BOVINE BESNOITIOSIS; TOXOPLASMA-GONDII; MICROSATELLITE ANALYSIS; GENETIC DIVERSITY; INFECTED CATTLE; APICOMPLEXA; CELL; VIRULENCE; PARASITES AB Background: Bovine besnoitiosis, caused by the protozoan Besnoitia besnoiti, reduces productivity and fertility of affected herds. Besnoitiosis continues to expand in Europe and no effective control tools are currently available. Experimental models are urgently needed. Herein, we describe for the first time the kinetics of standardised in vitro models for the B. besnoiti lytic cycle. This will aid to study the pathogenesis of the disease, in the screening for vaccine targets and drugs potentially useful for the treatment of besnoitiosis. Methods: We compared invasion and proliferation of one B. tarandi (from Finland) and seven B. besnoiti isolates (Bb-Spain1, Bb-Spain2, Bb-Israel, Bb-Evora03, Bb-Ger1, Bb-France, Bb-Italy2) in MARC-145 cell culture. Host cell invasion was studied at 4, 6, 8 and 24 h post infection (hpi), and proliferation characteristics were compared at 24, 48, 72, 96, 120, and 144 hpi. Results: In Besnoitia spp., the key parameters that determine the sequential adhesion-invasion, proliferation and egress steps are clearly distinct from those in the related apicomplexans Toxoplasma gondii and Neospora caninum. Besnoitia spp. host cell invasion is a rather slow process, since only 50 % of parasites were found intracellular after 3-6 h of exposure to host cells, and invasion still took place after 24 h. Invasion efficacy was significantly higher for Bb-France, Bb-Evora03 and Bb-Israel. In addition, the time span for endodyogeny to take place was as long as 18-35 h. Bb-Israel and B. tarandi isolates were most prolific, as determined by the tachyzoite yield at 72 hpi. The total tachyzoite yield could not be predicted neither by invasion-related parameters (velocity and half time invasion) nor by proliferation parameters (lag phase and doubling time (dT)). The lytic cycle of Besnoitia was asynchronous as evidenced by the presence of three different plaque-forming tachyzoite categories (lysis plaques, large and small parasitophorous vacuoles). Conclusions: This study provides first insights into the lytic cycle of B. besnoiti isolates and a standardised in vitro model that allows screening of drug candidates for the treatment of besnoitiosis. C1 [Frey, Caroline F.; Regidor-Cerrillo, Javier; Garcia-Lunar, Paula; Gutierrez-Exposito, Daniel; Ortega-Mora, Luis M.; Alvarez-Garcia, Gema] Univ Complutense Madrid, SALUVET, Fac Vet Sci, Dept Anim Hlth, Ciudad Univ S-N, E-28040 Madrid, Spain. [Frey, Caroline F.] Univ Bern, Vetsuisse Fac, Inst Parasitol, Langgass Str 122, CH-3012 Bern, Switzerland. [Marreros, Nelson] Univ Bern, Vetsuisse Fac, Ctr Fish & Wildlife Hlth, Langgass Str 122, CH-3012 Bern, Switzerland. [Schares, Gereon] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Epidemiol, Greifswald, Insel Riems, Germany. [Dubey, Jitender P.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Gentile, Arcangelo] Univ Bologna, Dept Vet Med Sci, Ozzano Dell Emilia, Italy. [Jacquiet, Philippe] Univ Toulouse, ENVT, INP, IHAP,Equipe Besnoitiose & Vecteurs,UMR1225, Toulouse, France. [Shkap, Varda] Kimron Vet Inst, Div Parasitol, POB 12, IL-50250 Bet Dagan, Israel. [Cortes, Helder] Univ Evora, ICAAM, Lab Parasitol Victor Caeiro, Nucleo Mitra, Apartado 94, P-7000554 Evora, Portugal. RP Frey, CF; Alvarez-Garcia, G (reprint author), Univ Complutense Madrid, SALUVET, Fac Vet Sci, Dept Anim Hlth, Ciudad Univ S-N, E-28040 Madrid, Spain.; Frey, CF (reprint author), Univ Bern, Vetsuisse Fac, Inst Parasitol, Langgass Str 122, CH-3012 Bern, Switzerland. EM caroline.frey@vetsuisse.unibe.ch; gemaga@vet.ucm.es RI Ortega-Mora, Luis-Miguel/F-1470-2016; Regidor Cerrillo, Javier/F-5736-2016; ALVAREZ-GARCIA, GEMA/E-3577-2016; OI Ortega-Mora, Luis-Miguel/0000-0002-4986-6783; Regidor Cerrillo, Javier/0000-0001-5831-6970; ALVAREZ-GARCIA, GEMA/0000-0002-6627-7577; Schares, Gereon/0000-0002-3217-289X; Marreros, Nelson/0000-0001-6802-4912 FU Spanish Ministry of Economy and Competitiveness [AGL2013-46442-R]; CYTED (Thematic Network) [113RT0469 Protozoovac]; Swiss National Science Foundation [PBBEP3_141435, PBBEP3_139398] FX This study was funded by the Spanish Ministry of Economy and Competitiveness (AGL2013-46442-R) and CYTED (Thematic Network 113RT0469 Protozoovac). CFF and NM are supported by the Swiss National Science Foundation (grants no. PBBEP3_141435 and PBBEP3_139398, respectively). We gratefully acknowledge Vanessa Navarro for her excellent technical assistance, and Andrew Hemphill for critical reading of the manuscript. NR 63 TC 0 Z9 0 U1 2 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 29 PY 2016 VL 9 AR 115 DI 10.1186/s13071-016-1405-9 PG 14 WC Parasitology SC Parasitology GA DF6GV UT WOS:000371454800001 PM 26928430 ER PT J AU Nonneman, DJ Schneider, JF Lents, CA Wiedmann, RT Vallet, JL Rohrer, GA AF Nonneman, Dan J. Schneider, James F. Lents, Clay A. Wiedmann, Ralph T. Vallet, Jeffrey L. Rohrer, Gary A. TI Genome-wide association and identification of candidate genes for age at puberty in swine SO BMC GENETICS LA English DT Article DE Bayes; Genome-wide association; Puberty; Pig; SNP; Swine ID LEAN GROWTH-RATE; QUANTITATIVE TRAIT LOCI; BODY-MASS INDEX; SUBSTANCE-P; BREAST-CANCER; REPRODUCTIVE TRAITS; ENDOMETRIAL CANCER; RISK-FACTORS; 1ST ESTRUS; MENARCHE AB Background: Reproductive efficiency has a great impact on the economic success of pork production. Gilts comprise a significant portion of breeding females and gilts that reach puberty earlier tend to stay in the herd longer and be more productive. About 10 to 30 % of gilts never farrow a litter and the most common reasons for removal are anestrus and failure to conceive. Puberty in pigs is usually defined as the female's first estrus in the presence of boar stimulation. Genetic markers associated with age at puberty will allow for selection on age at puberty and traits correlated with sow lifetime productivity. Results: Gilts (n = 759) with estrus detection measurements ranging from 140-240 days were genotyped using the Illumina PorcineSNP60 BeadChip and SNP were tested for significant effects with a Bayesian approach using GENSEL software. Of the available 8111 five-marker windows, 27 were found to be statistically significant with a comparison-wise error of P < 0.01. Ten QTL were highly significant at P < 0.005 level. Two QTL, one on SSC12 at 15 Mb and the other on SSC7 at 75 Mb, explained 16.87 % of the total genetic variance. The most compelling candidate genes in these two regions included the growth hormone gene (GH1) on SSC12 and PRKD1 on SSC7. Several loci confirmed associations previously identified for age at puberty in the pig and loci for age at menarche in humans. Conclusions: Several of the loci identified in this study have a physiological role for the onset of puberty and a genetic basis for sexual maturation in humans. Understanding the genes involved in regulation of the onset of puberty would allow for the improvement of reproductive efficiency in swine. Because age at puberty is a predictive factor for sow longevity and lifetime productivity, but not routinely measured or selected for in commercial herds, it would be beneficial to be able to use genomic or marker-assisted selection to improve these traits. C1 [Nonneman, Dan J.; Schneider, James F.; Lents, Clay A.; Wiedmann, Ralph T.; Vallet, Jeffrey L.; Rohrer, Gary A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Nonneman, DJ (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Dan.Nonneman@ars.usda.gov FU CRIS of the Agricultural Research Service, a division of the US Department of Agriculture [3040-31000-094-00] FX The authors acknowledge the U.S. Meat Animal Research Center (USMARC, Clay Center, NE) swine crew for expert animal husbandry and data collection, Linda Parnell (USMARC, Clay Center, NE) for manuscript preparation, Kris Simmerman (USMARC, Clay Center, NE) for DNA extraction, and Tad Sonstegard, Alicia Bertles, and Steven Schroeder at the Bovine Functional Genomics Laboratory (BARC, Beltsville, MD) for scanning Illumina PorcineSNP60 BeadChips. This work was funded by CRIS #3040-31000-094-00 of the Agricultural Research Service, a division of the US Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 91 TC 0 Z9 0 U1 4 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD FEB 29 PY 2016 VL 17 AR 50 DI 10.1186/s12863-016-0352-y PG 9 WC Genetics & Heredity SC Genetics & Heredity GA DF1RQ UT WOS:000371117300002 PM 26923368 ER PT J AU Jansky, SH Roble, J Spooner, DM AF Jansky, Shelley H. Roble, Jacob Spooner, David M. TI Solanum clarum and S. morelliforme as Novel Model Species for Studies of Epiphytism SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE epiphyte; mineral uptake; Solanum clarum; Solanum morelliforme ID BIOGEOGRAPHIC PREDICTIVITY RESISTANCE; CULTIVATED POTATO; WILD RELATIVES; TAXONOMIC PREDICTIVITY; VASCULAR EPIPHYTES; MINERAL-NUTRITION; NUTRIENT STATUS; TUBER CALCIUM; COSTA-RICA; PHOSPHORUS AB The natural history of epiphytic plant species has been extensively studied. However, little is known about the physiology and genetics of epiphytism. This is due to difficulties associated with growing epiphytic plants and the lack of tools for genomics studies and genetic manipulations. In this study, tubers were generated from 223 accessions of 42 wild potato Solanurn species, including the epiphytic species S. morelliforme and its sister species S. clarum. Lyophilized samples were analyzed for 12 minerals using inductively coupled plasma optical emission spectrometry. Mineral levels in tubers of S. morelliforme and S. clarum were among the highest for 10 out of the 12 elements evaluated. These two wild potato relatives are native to southern Mexico and Central America and live as epiphytes or in epiphytic-like conditions. We propose the use of S. morelliforme and S. clarum as model organisms for the study of mineral uptake efficiency. They have a short life cycle, can be propagated vegetatively via tubers or cuttings, and can be easily grown in controlled environments. In addition, genome sequence data are available for potato. Transgenic manipulations and somatic fusions will allow the movement of genes from these epiphytes to cultivated potato. C1 [Jansky, Shelley H.; Spooner, David M.] USDA ARS, Vegetable Crops Res Unit, Madison, WI USA. [Jansky, Shelley H.; Roble, Jacob; Spooner, David M.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. RP Jansky, SH (reprint author), USDA ARS, Vegetable Crops Res Unit, Madison, WI USA.; Jansky, SH (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov NR 37 TC 0 Z9 0 U1 5 U2 5 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 29 PY 2016 VL 7 AR 231 DI 10.3389/fpls.2016.00231 PG 5 WC Plant Sciences SC Plant Sciences GA DF0WY UT WOS:000371061100001 PM 26973674 ER PT J AU Cerin, E Baranowski, T Barnett, A Butte, N Hughes, S Lee, RE Mendoza, JA Thompson, D O'Connor, TM AF Cerin, Ester Baranowski, Tom Barnett, Anthony Butte, Nancy Hughes, Sheryl Lee, Rebecca E. Mendoza, Jason A. Thompson, Debbe O'Connor, Teresia Margareta TI Places where preschoolers are (in)active: an observational study on Latino preschoolers and their parents using objective measures SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Physical activity locations; Global positioning system; Accelerometry; Preschool-aged children; Parenting practices; Neighborhood perceptions; Sedentary behavior ID GLOBAL POSITIONING SYSTEMS; PHYSICAL-ACTIVITY; YOUNG-CHILDREN; BUILT ENVIRONMENT; AGED CHILDREN; ADOLESCENTS; OBESITY; TIME; ACCELEROMETER; HEALTH AB Background: To combat the disproportionately higher risk of childhood obesity in Latino preschool-aged children, multilevel interventions targeting physical (in) activity are needed. These require the identification of environmental and psychosocial determinants of physical (in) activity for this ethnic group. The objectives were to examine differences in objectively-measured physical activity and sedentary behavior across objectively-determined types of locations in Latino preschool-aged children; and determine whether the differences in physical activity by location were greater in children of parents with higher neighborhood-safety perceptions and physical activity-supportive parenting practices. Methods: An observational field study was conducted in Houston (Texas, USA) from August 2011 to April 2012. A purposive sample of Latino children aged 3-5 years and one of their parents (n = 84) were recruited from Census block groups in Houston (Texas) stratified by objectively-assessed high vs. low traffic and crime safety. Seventy-three children provided valid data. Time spent outdoors/indoors tagged with geographic locations was coded into location types based on objective data collected using Global Positioning Systems units that children wore > 8 hr/day for a week. Physical activity parenting practices, perceived neighborhood-safety, and demographics were reported by parents. Time spent in sedentary behavior and moderate-to-vigorous physical activity was measured based on objective data collected using accelerometers (motion sensors) that children wore > 8 hr/day for a week. Results: The odds of children engaging in moderate-to-vigorous physical activity were 43 % higher when outdoors than indoors (95 % confidence interval: 1.30, 1.58), and the odds of being sedentary were 14 % lower when outdoors compared to indoors (95 % confidence intervals: 0.81, 0.91). This difference depended on parental neighborhood-safety perceptions and parenting practices. Children were most active in parks/playgrounds (30 % of the time spent in moderate-to-vigorous physical activity) and least active in childcare/school settings (8 % of the time spent in moderate-to-vigorous physical activity). Conclusions: Objectively-assessed time spent in specific locations is correlated with physical activity and sedentary behavior in Latino preschoolers. Interventions and policies should identify ways to engage Latino preschool-aged children in more physical activity and less sedentary behavior while in childcare, and encourage parents to spend more time with their young children in parks/playgrounds and other safe outdoor places. C1 [Cerin, Ester; Barnett, Anthony] Australian Catholic Univ, Inst Hlth & Ageing, Melbourne, Vic 3000, Australia. [Cerin, Ester] Univ Hong Kong, Sch Publ Hlth, Hong Kong, Hong Kong, Peoples R China. [Baranowski, Tom; Butte, Nancy; Hughes, Sheryl; Thompson, Debbe; O'Connor, Teresia Margareta] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lee, Rebecca E.] Arizona State Univ, Coll Nursing & Hlth Innovat, Ctr Hlth Promot & Dis Prevent, Phoenix, AZ USA. [Mendoza, Jason A.] Univ Washington, Dept Pediat, Seattle, WA 98195 USA. [Mendoza, Jason A.] Seattle Childrens Res Inst, Seattle, WA USA. RP Cerin, E (reprint author), Australian Catholic Univ, Inst Hlth & Ageing, Melbourne, Vic 3000, Australia.; Cerin, E (reprint author), Univ Hong Kong, Sch Publ Hlth, Hong Kong, Hong Kong, Peoples R China. EM ecerin@hku.hk RI Cerin, Ester/L-1271-2015; OI Cerin, Ester/0000-0002-7599-165X; Barnett, Anthony/0000-0002-6320-4073; Baranowski, Tom/0000-0002-0653-2222 FU Australian Research Council Future Fellowship [140100085]; NIH-Eunice Kennedy Shriver National Institute of Child Health & Human Development [R21HD060925] FX EC is supported by an Australian Research Council Future Fellowship (# 140100085). This study was funded by NIH-Eunice Kennedy Shriver National Institute of Child Health & Human Development (R21HD060925). This work is a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, BCM through a cooperative agreement (USDA/ARS 6250-51000-053-20S). The contents of this publication do not necessarily reflect the views or policies of the USDA or NIH, nor does mention of organizations imply endorsement from the US government. The study sponsors had no role in study design and conduct of the study; collection, management, analysis, and interpretation of data; and preparation, review or approval of the manuscript. NR 60 TC 1 Z9 1 U1 4 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD FEB 29 PY 2016 VL 13 AR 29 DI 10.1186/s12966-016-0355-0 PG 12 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA DF1TP UT WOS:000371122400001 PM 26928561 ER PT J AU Li, Y Manolache, S Qiu, Y Sarmadi, M AF Li, Ying Manolache, Sorin Qiu, Yiping Sarmadi, Majid TI Effect of atmospheric pressure plasma treatment condition on adhesion of ramie fibers to polypropylene for composite SO APPLIED SURFACE SCIENCE LA English DT Article DE Dielectric barrier discharge; Response surface; Ramie fibers; Ethanol vapor; Interfacial adhesion ID MECHANICAL-PROPERTIES; FASTNESS PROPERTIES; COLOR STRENGTH; NATURAL FIBER; DYE; QUERCETIN; NILOTICA AB In order to improve the interfacial adhesion between hydrophilic ramie fibers and hydrophobic polypropylene (PP) matrices, ramie fibers are modified by atmospheric pressure dielectric barrier discharge (DBD) plasma with our continuous ethanol flow technique in helium environment. A central composite design of experiments with different plasma processing parameter combinations (treatment current, treatment time and ethanol flow rate) is applied to find the most influential parameter and to obtain the best modification effect. Field emission scanning electron microscope (SEM) shows the roughened surfaces of ramie fibers from the treated groups due to plasma etching effect. Dynamic contact angle analysis (DCAA) demonstrates that the wettability of the treated fibers drastically decreases. Microbond pullout test shows that the interfacial shear strength (IFSS) between treated ramie fibers and PP matrices increases significantly. Residual gas analysis (RGA) confirms the creation of ethyl groups during plasma treatment. This study shows that our continuous ethanol flow technique is effective in the plasma modification process, during which the ethanol flow rate is the most influential parameter but all parameters have simultaneous influence on plasma modification effect of ramie fibers. (C) 2015 Elsevier B.V. All rights reserved. C1 [Li, Ying] Jiaxing Univ, Coll Mat & Text Engn, Jiaxing 34033, Peoples R China. [Li, Ying; Manolache, Sorin; Sarmadi, Majid] Ctr Plasma Aided Mfg, Madison, WI 53706 USA. [Li, Ying; Sarmadi, Majid] Univ Wisconsin, Sch Human Ecol, 1300 Linden Dr, Madison, WI 53706 USA. [Manolache, Sorin] Forest Prod Lab, Madison, WI 53726 USA. [Qiu, Yiping] Donghua Univ, Coll Text, Shanghai 201620, Peoples R China. [Sarmadi, Majid] Univ Wisconsin, Mat Sci Program, Madison, WI 53706 USA. RP Sarmadi, M (reprint author), Univ Wisconsin, Sch Human Ecol, 1300 Linden Dr, Madison, WI 53706 USA.; Qiu, Y (reprint author), Donghua Univ, Coll Text, Shanghai 201620, Peoples R China. EM ypqiu@dhu.edu.cn; majidsar@wisc.edu FU Chinese Scholarship Council; Hatch Federal Funding [S-1054] FX This work was supported by the Chinese Scholarship Council and S-1054 Hatch Federal Funding. The authors acknowledge use of instrumentation supported by the University of Wisconsin, College of Engineering and the following NSF CENTERS Material Research and Engineering Center (NSF-DMR-1121288) Nanoscale Science and Engineering Center (NSF-DMR-0832760). NR 26 TC 0 Z9 0 U1 6 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-4332 EI 1873-5584 J9 APPL SURF SCI JI Appl. Surf. Sci. PD FEB 28 PY 2016 VL 364 BP 294 EP 301 DI 10.1016/j.apsusc.2015.12.092 PG 8 WC Chemistry, Physical; Materials Science, Coatings & Films; Physics, Applied; Physics, Condensed Matter SC Chemistry; Materials Science; Physics GA DD5IW UT WOS:000369957800039 ER PT J AU Bai, KX Chang, NB Gao, W AF Bai, Kaixu Chang, Ni-Bin Gao, Wei TI Quantification of relative contribution of Antarctic ozone depletion to increased austral extratropical precipitation during 1979-2013 SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE climate change; precipitation; ozone depletion; pattern recognition ID EXTREME LEARNING-MACHINE; FINDING COUPLED PATTERNS; CLIMATE-CHANGE; WEST ANTARCTICA; RECOVERY; MODELS; HOLE; ASSIMILATION; TEMPERATURE; REGRESSION AB Attributing the observed climate changes to relevant forcing factors is critical to predicting future climate change scenarios. Precipitation observations in the Southern Hemisphere indicate an apparent moistening pattern over the extratropics during the time period 1979 to 2013. To investigate the predominant forcing factor in triggering such an observed wetting climate pattern, precipitation responses to four climatic forcing factors, including Antarctic ozone, water vapor, sea surface temperature (SST), and carbon dioxide, were assessed quantitatively in sequence through an inductive approach. Coupled time-space patterns between the observed austral extratropical precipitation and each climatic forcing factor were firstly diagnosed by using the maximum covariance analysis (MCA). With the derived time series from each coupled MCA modes, statistical relationships were established between extratropical precipitation variations and each climatic forcing factor by using the extreme learning machine. Based on these established statistical relationships, sensitivity tests were conducted to estimate precipitation responses to each climatic forcing factor quantitatively. Quantified differential contribution with respect to those climatic forcing factors may explain why the observed austral extratropical moistening pattern is primarily driven by the Antarctic ozone depletion, while mildly modulated by the cooling effect of equatorial Pacific SST and the increased greenhouse gases, respectively. C1 [Bai, Kaixu; Gao, Wei] E China Normal Univ, Minist Educ, Key Lab Geog Informat Sci, Shanghai 200062, Peoples R China. [Bai, Kaixu; Chang, Ni-Bin] Univ Cent Florida, Dept Civil Environm & Construct Engn, Orlando, FL 32816 USA. [Gao, Wei] Colorado State Univ, Nat Resource Ecol Lab, USDA UV B Monitoring & Res Program, Ft Collins, CO 80523 USA. [Gao, Wei] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. RP Chang, NB (reprint author), Univ Cent Florida, Dept Civil Environm & Construct Engn, Orlando, FL 32816 USA. EM nchang@ucf.edu RI Fan, Jiwen/E-9138-2011 FU Science and Technology Commission of Shanghai Municipality [15dz1207805, 13231203804]; USDA NIFA [2015-34263-24070] FX The GPCP Version 2.2 Combination Data were obtained from the NASA Goddard Space Flight Center, the ERA-Interim Reanalysis data from the ECMWF, and the carbon dioxide data from the NOAA/ESRL. This work was supported by the Science and Technology Commission of Shanghai Municipality (15dz1207805 and 13231203804) and USDA NIFA (2015-34263-24070). NR 61 TC 1 Z9 1 U1 6 U2 16 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD FEB 27 PY 2016 VL 121 IS 4 BP 1459 EP 1474 DI 10.1002/2015JD024247 PG 16 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DH7MK UT WOS:000372977900005 ER PT J AU Parajuli, SP Zobeck, TM Kocurek, G Yang, ZL Stenchikov, GL AF Parajuli, Sagar Prasad Zobeck, Ted M. Kocurek, Gary Yang, Zong-Liang Stenchikov, Georgiy L. TI New insights into the wind-dust relationship in sandblasting and direct aerodynamic entrainment from wind tunnel experiments SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE dust emission; wind tunnel experiments; sandblasting; aerodynamic entrainment; aerosols; surface roughness ID SIZE DISTRIBUTIONS; SALTATION BOMBARDMENT; ROUGHNESS ELEMENTS; RIVER INCISION; EROSION; SOIL; EMISSION; AEROSOLS; MODEL; THRESHOLD AB Numerous parameterizations have been developed for predicting wind erosion, yet the physical mechanism of dust emission is not fully understood. Sandblasting is thought to be the primary mechanism, but recent studies suggest that dust emission by direct aerodynamic entrainment can be significant under certain conditions. In this work, using wind tunnel experiments, we investigated some of the lesser understood aspects of dust emission in sandblasting and aerodynamic entrainment for three soil types, namely, clay, silty clay loam, and clay loam. First, we explored the role of erodible surface roughness on dust emitted by aerodynamic entrainment. Second, we compared the emitted dust concentration in sandblasting and aerodynamic entrainment under a range of wind friction velocities. Finally, we explored the sensitivity of emitted dust particle size distribution (PSD) to soil type and wind friction velocity in these two processes. The dust concentration in aerodynamic entrainment showed strong positive correlation, no significant correlation, and weak negative correlation, for the clay, silty clay loam, and clay loam, respectively, with the erodible soil surface roughness. The dust in aerodynamic entrainment was significant constituting up to 28.3, 41.4, and 146.4% compared to sandblasting for the clay, silty clay loam, and clay loam, respectively. PSD of emitted dust was sensitive to soil type in both sandblasting and aerodynamic entrainment. PSD was sensitive to the friction velocity in aerodynamic entrainment but not in sandblasting. Our results highlight the need to consider the details of sandblasting and direct aerodynamic entrainment processes in parameterizing dust emission in global/regional climate models. C1 [Parajuli, Sagar Prasad; Kocurek, Gary; Yang, Zong-Liang] Univ Texas Austin, Jackson Sch Geosci, Austin, TX 78712 USA. [Zobeck, Ted M.] ARS, USDA, Lubbock, TX USA. [Stenchikov, Georgiy L.] King Abdullah Univ Sci & Technol, Phys Sci & Engn Div, Thuwal, Saudi Arabia. RP Yang, ZL (reprint author), Univ Texas Austin, Jackson Sch Geosci, Austin, TX 78712 USA. EM liang@jsg.utexas.edu RI Yang, Zong-Liang/B-4916-2011 FU King Abdullah University of Science and Technology FX This work was funded by the third-round grant from King Abdullah University of Science and Technology. We thank Dean Holder from USDA-ARS/Wind Erosion and Water Conservation Research Unit, Lubbock, Texas, for providing technical assistance during the experiments. We are extremely grateful to the anonymous reviewers for providing many constructive comments, which have substantially improved the quality of the manuscript. The first author is grateful to Jasper Kok of UCLA, David Mohrig of UT-Austin, and John E. Stout of USDA-ARS for providing critical insights into the experimental results. We also thank Adam Papendieck of UT-Austin for proofreading the manuscript. All the data used in this study can be obtained by contacting the first author by e-mail (psagar@utexas.edu). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 75 TC 3 Z9 3 U1 5 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD FEB 27 PY 2016 VL 121 IS 4 BP 1776 EP 1792 DI 10.1002/2015JD024424 PG 17 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DH7MK UT WOS:000372977900025 ER PT J AU Dore, S Fry, DL Collins, BM Vargas, R York, RA Stephens, SL AF Dore, Sabina Fry, Danny L. Collins, Brandon M. Vargas, Rodrigo York, Robert A. Stephens, Scott L. TI Management Impacts on Carbon Dynamics in a Sierra Nevada Mixed Conifer Forest SO PLOS ONE LA English DT Article ID PONDEROSA PINE PLANTATION; SOIL CO2 EFFLUX; FUEL REDUCTION TREATMENTS; WESTERN UNITED-STATES; PRESCRIBED FIRE; DRY FORESTS; THINNING TREATMENT; TREE MORTALITY; NATIONAL FIRE; CLEAR-CUT AB Forest ecosystems can act as sinks of carbon and thus mitigate anthropogenic carbon emissions. When forests are actively managed, treatments can alter forests carbon dynamics, reducing their sink strength and switching them from sinks to sources of carbon. These effects are generally characterized by fast temporal dynamics. Hence this study monitored for over a decade the impacts of management practices commonly used to reduce fire hazards on the carbon dynamics of mixed-conifer forests in the Sierra Nevada, California, USA. Soil CO2 efflux, carbon pools (i.e. soil carbon, litter, fine roots, tree biomass), and radial tree growth were compared among un-manipulated controls, prescribed fire, thinning, thinning followed by fire, and two clear-cut harvested sites. Soil CO2 efflux was reduced by both fire and harvesting (ca. 15%). Soil carbon content (upper 15 cm) was not significantly changed by harvest or fire treatments. Fine root biomass was reduced by clear-cut harvest (60-70%) but not by fire, and the litter layer was reduced 80% by clear-cut harvest and 40% by fire. Thinning effects on tree growth and biomass were concentrated in the first year after treatments, whereas fire effects persisted over the seven-year post-treatment period. Over this period, tree radial growth was increased (25%) by thinning and reduced (12%) by fire. After seven years, tree biomass returned to pre-treatment levels in both fire and thinning treatments; however, biomass and productivity decreased 30%-40% compared to controls when thinning was combined with fire. The clear-cut treatment had the strongest impact, reducing ecosystem carbon stocks and delaying the capacity for carbon uptake. We conclude that post-treatment carbon dynamics and ecosystem recovery time varied with intensity and type of treatments. Consequently, management practices can be selected to minimize ecosystem carbon losses while increasing future carbon uptake, resilience to high severity fire, and climate related stresses. C1 [Dore, Sabina; Fry, Danny L.; York, Robert A.; Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Collins, Brandon M.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Coll Nat Resources, Berkeley, CA 94720 USA. [Vargas, Rodrigo] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19717 USA. [York, Robert A.] Univ Calif Berkeley, Ctr Forestry, Georgetown, CA USA. RP Dore, S (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM sabina.dore@gmail.com RI Vargas, Rodrigo/C-4720-2008 OI Vargas, Rodrigo/0000-0001-6829-5333 FU Joint Fire Sciences Program Project [10-1-10-21]; USDA [2014-67003-22070] FX This work was supported by the Joint Fire Sciences Program Project #10-1-10-21, received by SS. RV acknowledges partial support from USDA (2014-67003-22070). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 0 Z9 0 U1 6 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 26 PY 2016 VL 11 IS 2 AR e0150256 DI 10.1371/journal.pone.0150256 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3UU UT WOS:000371274400110 PM 26918460 ER PT J AU Manoukis, NC AF Manoukis, Nicholas C. TI To Catch a Fly: Landing and Capture of Ceratitis capitata in a Jackson Trap with and without an Insecticide SO PLOS ONE LA English DT Article ID MEDITERRANEAN FRUIT-FLIES; EFFECTIVE ATTRACTION RADIUS; BACTROCERA-DORSALIS; DIPTERA TEPHRITIDAE; METHYL EUGENOL; PLASTIC MATRIX; MALE LURES; TRIMEDLURE; ERADICATION; POPULATIONS AB Attractant-based traps are a cornerstone of detection, delimitation and eradication programs for pests such as tephritid fruit flies. The ideal trap and lure combination has high attraction (it brings insects to the trap from a distance) and high capture efficiency (it has a high probability of capturing the insect once it arrives at the trap). We examined the effect of an insecticide (DDVP) in combination with a pheromone lure (trimedlure) on capture of Ceratitis capitata using 1) digital images of surfaces of a Jackson trap analyzed via computer vision, and 2) counts of the number of flies caught in the trap and in the area under the trap. Our results indicate no significant difference in trap capture without or with insecticide (means +/- SD = 324 +/- 135 and 356 +/- 108, respectively). However, significantly more dead flies were found around the trap with insecticide (92 +/- 53 with insecticide compared with 35 +/- 22 without), suggesting a possible decrease in trap efficiency due to mortality before insects enter the trap. Indeed, the average number of flies detected on all surfaces of the traps with insecticide was lower than that for lure-only (4.15 +/- 0.39 vs 8.30 +/- 1.18), and both were higher than control (no lure: 0.76 +/- 0.08). We found that the majority of fly sightings, 71% of the total, occurred on the inside panels of the lure-only traps, suggesting that increased efficiency of the Jackson trap may be obtained by adding a contact insecticide to those surfaces. C1 [Manoukis, Nicholas C.] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI USA. RP Manoukis, NC (reprint author), USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI USA. EM nicholas.manoukis@ars.usda.gov FU USDA-ARS; intramural research arm of USDA FX This study was funded by USDA-ARS, the intramural research arm of USDA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 0 Z9 0 U1 2 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 26 PY 2016 VL 11 IS 2 AR e0149869 DI 10.1371/journal.pone.0149869 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3UU UT WOS:000371274400051 PM 26918513 ER PT J AU Roy, SK Cho, SW Kwon, SJ Kamal, AM Kim, SW Oh, MW Lee, MS Chung, KY Xin, ZG Woo, SH AF Roy, Swapan Kumar Cho, Seong-Woo Kwon, Soo Jeong Kamal, Abu Hena Mostafa Kim, Sang-Woo Oh, Myeong-Won Lee, Moon-Soon Chung, Keun-Yook Xin, Zhanguo Woo, Sun-Hee TI Morpho-Physiological and Proteome Level Responses to Cadmium Stress in Sorghum SO PLOS ONE LA English DT Article ID GLUTATHIONE S-TRANSFERASES; ALGAE SARGASSUM-FUSIFORME; INDUCED OXIDATIVE STRESS; ARABIDOPSIS-THALIANA; THLASPI-CAERULESCENS; METAL ACCUMULATION; ANALYSIS PROVIDES; DROUGHT-STRESS; PLANT-RESPONSE; LEAF PROTEOME AB Cadmium (Cd) stress may cause serious morphological and physiological abnormalities in addition to altering the proteome in plants. The present study was performed to explore Cd-induced morpho-physiological alterations and their potential associated mechanisms in Sorghum bicolor leaves at the protein level. Ten-day-old sorghum seedlings were exposed to different concentrations (0, 100, and 150 mu M) of CdCl2, and different morpho-physiological responses were recorded. The effects of Cd exposure on protein expression patterns in S. bicolor were investigated using two-dimensional gel electrophoresis (2-DE) in samples derived from the leaves of both control and Cd-treated seedlings. The observed morphological changes revealed that the plants treated with Cd displayed dramatically altered shoot lengths, fresh weights and relative water content. In addition, the concentration of Cd was markedly increased by treatment with Cd, and the amount of Cd taken up by the shoots was significantly and directly correlated with the applied concentration of Cd. Using the 2-DE method, a total of 33 differentially expressed protein spots were analyzed using MALDI-TOF/TOF MS. Of these, treatment with Cd resulted in significant increases in 15 proteins and decreases in 18 proteins. Major changes were absorbed in the levels of proteins known to be involved in carbohydrate metabolism, transcriptional regulation, translation and stress responses. Proteomic results revealed that Cd stress had an inhibitory effect on carbon fixation, ATP production and the regulation of protein synthesis. Our study provides insights into the integrated molecular mechanisms involved in responses to Cd and the effects of Cd on the growth and physiological characteristics of sorghum seedlings. We have aimed to provide a reference describing the mechanisms involved in heavy metal damage to plants. C1 [Roy, Swapan Kumar; Kwon, Soo Jeong; Kim, Sang-Woo; Woo, Sun-Hee] Chungbuk Natl Univ, Dept Crop Sci, Cheongju, South Korea. [Cho, Seong-Woo] Rural Dev Adm, Div Rice Res, Natl Inst Crop Sci, Suwon, South Korea. [Kamal, Abu Hena Mostafa] Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA. [Oh, Myeong-Won] Rural Dev Adm, Natl Acad Agr Sci, Natl Agrobiodivers Ctr, Jeonju, South Korea. [Lee, Moon-Soon] Chungbuk Natl Univ, Dept Crop Sci, Cheongju, South Korea. [Chung, Keun-Yook] Chungbuk Natl Univ, Dept Environm & Biol Chem, Cheongju, South Korea. [Xin, Zhanguo] ARS, Plant Stress & Germplasm Dev Unit, USDA, 3810 4th St, Lubbock, TX USA. RP Woo, SH (reprint author), Chungbuk Natl Univ, Dept Crop Sci, Cheongju, South Korea.; Lee, MS (reprint author), Chungbuk Natl Univ, Dept Crop Sci, Cheongju, South Korea. EM mslee416@chungbuk.ac.kr; shwoo@chungbuk.ac.kr RI Roy, Swapan Kumar/R-2427-2016; OI Roy, Swapan Kumar/0000-0002-3837-4011; Cho, Seong-Woo/0000-0001-7900-4781; Woo, Sun Hee/0000-0002-6219-1688 FU Rural Development Administration, Republic of Korea [PJ009101012014] FX This work was supported by a grant from the Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea. Grant No: PJ009101012014. URL: http://atis.rda.go.kr/rdais/main/main.vw. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 114 TC 1 Z9 1 U1 5 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 26 PY 2016 VL 11 IS 2 AR e0150431 DI 10.1371/journal.pone.0150431 PG 27 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3UU UT WOS:000371274400128 PM 26919231 ER PT J AU Guo, JF He, KL Hellmich, RL Bai, SX Zhang, TT Liu, YJ Ahmed, T Wang, ZY AF Guo, Jingfei He, Kanglai Hellmich, Richard L. Bai, Shuxiong Zhang, Tiantao Liu, Yunjun Ahmed, Tofael Wang, Zhenying TI Field trials to evaluate the effects of transgenic cry1le maize on the community characteristics of arthropod natural enemies SO SCIENTIFIC REPORTS LA English DT Article ID BACILLUS-THURINGIENSIS TOXIN; PRINCIPAL RESPONSE CURVES; ASIAN CORN-BORER; BT COTTON; MULTIVARIATE ANALYSES; NONTARGET ARTHROPODS; BIOLOGICAL-CONTROL; CROSS-RESISTANCE; APHID PREY; PLANTS AB Possible non-target effect of transgenic cry1Ie maize exerts on natural enemy community biodiversity in the field is unresolved. In the present study, a 2-yr comparison of transgenic cry1Ie maize (Event IE09S034, Bt maize) and its near isoline (Zong 31, non-Bt maize) on natural enemy community biodiversity were compared with whole plant inspections, pitfall traps and suction sampler. Natural enemy diversity indices (Shannon-Wiener', Simpson's and Pielou's index) and abundance suggested there were no significant differences between the two types of maize. The only exceptions were the Pielou's index for whole plant inspections in 2013 and abundance for pitfall traps in 2012, which were significantly higher in Bt maize than those of non-Bt maize. The main species of natural enemies were identical in Bt and non-Bt maize plots for each method and the three methods combined. For whole plant inspections, Bt maize had no time-dependent effect on the entire arthropod natural enemy community, and also no effect on community dissimilarities between Bt and non-Bt maize plots. These results suggested that despite the presence of a relatively minor difference in natural enemy communities between Bt and non-Bt maize, transgenic cry1le maize had little, if any, effect on natural enemy community biodiversity. C1 [Guo, Jingfei; He, Kanglai; Bai, Shuxiong; Zhang, Tiantao; Ahmed, Tofael; Wang, Zhenying] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, MOA CABI Joint Lab Biosafety, Inst Plant Protect, Beijing 100193, Peoples R China. [Hellmich, Richard L.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Liu, Yunjun] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China. [Ahmed, Tofael] Bangladesh Sugarcane Res Inst, Entomol Div, Ishurdi, Pabna, Bangladesh. RP Wang, ZY (reprint author), Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, MOA CABI Joint Lab Biosafety, Inst Plant Protect, Beijing 100193, Peoples R China. EM zywang@ippcaas.cn NR 71 TC 2 Z9 2 U1 13 U2 19 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 26 PY 2016 VL 6 AR 22102 DI 10.1038/srep22102 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DE9AL UT WOS:000370927700002 PM 26915985 ER PT J AU Zhu, LH Krens, F Smith, MA Li, XY Qi, WC van Loo, EN Iven, T Feussner, I Nazarenus, TJ Huai, DX Taylor, DC Zhou, XR Green, AG Shockey, J Klasson, KT Mullen, RT Huang, BQ Dyer, JM Cahoon, EB AF Zhu, Li-Hua Krens, Frans Smith, Mark A. Li, Xueyuan Qi, Weicong van Loo, Eibertus N. Iven, Tim Feussner, Ivo Nazarenus, Tara J. Huai, Dongxin Taylor, David C. Zhou, Xue-Rong Green, Allan G. Shockey, Jay Klasson, K. Thomas Mullen, Robert T. Huang, Bangquan Dyer, John M. Cahoon, Edgar B. TI Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production SO SCIENTIFIC REPORTS LA English DT Article ID AGROBACTERIUM-MEDIATED TRANSFORMATION; TRANSGENIC PLANTS; CRAMBE-ABYSSINICA; BRASSICA-CARINATA; RAPESEED OIL; ERUCIC-ACID; FATTY-ACID; SPERM-WHALE; RAT-HEART; JOJOBA AB Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds. C1 [Zhu, Li-Hua; Li, Xueyuan] Swedish Univ Agr Sci, Dept Plant Breeding, Box 101, SE-23053 Alnarp, Sweden. [Krens, Frans; Qi, Weicong; van Loo, Eibertus N.] Wageningen UR Plant Breeding, POB 386, NL-6700 AJ Wageningen, Netherlands. [Smith, Mark A.; Taylor, David C.] Natl Res Council Canada, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada. [Iven, Tim; Feussner, Ivo] Univ Gottingen, Albrecht von Haller Inst Plant Sci, Dept Plant Biochem, Justus von Liebig Weg 11, D-37077 Gottingen, Germany. [Feussner, Ivo] Univ Gottingen, Goettingen Ctr Mol Biosci GZMB, Dept Plant Biochem, Justus von Liebig Weg 11, D-37077 Gottingen, Germany. [Feussner, Ivo] Univ Gottingen, ICASEC, Dept Plant Biochem, Justus von Liebig Weg 11, D-37077 Gottingen, Germany. [Nazarenus, Tara J.; Huai, Dongxin; Cahoon, Edgar B.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA. [Nazarenus, Tara J.; Huai, Dongxin; Cahoon, Edgar B.] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA. [Huai, Dongxin] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. [Huai, Dongxin] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China. [Zhou, Xue-Rong] CSIRO Food & Nutr, Sydney, NSW, Australia. [Zhou, Xue-Rong] CSIRO Agr, Canberra, ACT, Australia. [Shockey, Jay; Klasson, K. Thomas] ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70124 USA. [Mullen, Robert T.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Huang, Bangquan] Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China. [Dyer, John M.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Zhu, LH (reprint author), Swedish Univ Agr Sci, Dept Plant Breeding, Box 101, SE-23053 Alnarp, Sweden.; Cahoon, EB (reprint author), Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA.; Cahoon, EB (reprint author), Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA. EM Li-Hua.Zhu@slu.se; ecahoon2@unl.edu RI Zhou, Xue-Rong/D-4706-2009 OI Zhou, Xue-Rong/0000-0003-3717-2984 FU EU; Swedish Research Council FORMAS; VINNOVA; Swedish Foundation for Strategic Research (SSF); Swedish University of Agricultural Sciences; USDA-NIFA [2009-05988]; National Science Foundation [Plant Genome IOS-13-39385]; Saskatchewan Agriculture Development Fund; Western Economic Partnership-Prairie Gold; National Research Council Canada FX This work is a part of EU-ICON project and financed by EU (FP7-KBBE-2007-1), The Swedish Research Council FORMAS, VINNOVA, Swedish Foundation for Strategic Research (SSF) and Swedish University of Agricultural Sciences. We wish to thank the ICON project coordinator Prof. Sten Stymne for his great support to this work and his valuable comments and discussion on the manuscript; The Rural Economy and Agricultural Societies for performing the crambe field trial; David Taylor and Mark Smith thank the Saskatchewan Agriculture Development Fund, Western Economic Partnership-Prairie Gold and the National Research Council Canada, for support of the carinata work. Xue-Rong Zhou and Allan Green thank New South Wales Department of Agriculture, Australia, for allowing collection of developing and mature seeds from jojoba plantations, and Edgar Cahoon thanks USDA-NIFA (Grant no. 2009-05988) and National Science Foundation (Plant Genome IOS-13-39385) for support of the camelina research. NR 38 TC 2 Z9 2 U1 5 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 26 PY 2016 VL 6 AR 22181 DI 10.1038/srep22181 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DE8DH UT WOS:000370865500001 PM 26916792 ER PT J AU Yang, LM Ji, JH Harris-Shultz, KR Wang, H Wang, HL Abd-Allah, EF Luo, YM Hu, XY AF Yang, Liming Ji, Jianhui Harris-Shultz, Karen R. Wang, Hui Wang, Hongliang Abd-Allah, Elsayed F. Luo, Yuming Hu, Xiangyang TI The Dynamic Changes of the Plasma Membrane Proteins and the Protective Roles of Nitric Oxide in Rice Subjected to Heavy Metal Cadmium Stress SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE rice; cadmium pollution; nitric oxide; reactive oxygen species; lipid hydrolysis; quantitative proteomics ID ARABIDOPSIS MESOPHYLL PROTOPLASTS; PLANT WATER RELATIONS; REACTIVE OXYGEN; PHOSPHOLIPASE-D; SIGNAL-TRANSDUCTION; OXIDATIVE STRESS; STOMATAL CLOSURE; ANALYSIS REVEALS; INDUCED TOXICITY; GENE-EXPRESSION AB The heavy metal cadmium is a common environmental contaminant in soils and has adverse effects on crop growth and development. The signaling processes in plants that initiate cellular responses to environmental stress have been shown to be located in the plasma membrane (PM). A better understanding of the PM proteome in response to environmental stress might provide new insights for improving stress tolerant crops. Nitric oxide (NO) is reported to be involved in the plant response to cadmium (Cd) stress. To further investigate how NO modulates protein changes in the plasma membrane during Cd stress, a quantitative proteomics approach based on isobaric tags for relative and absolute quantification (iTRAQ) was used to identify differentially regulated proteins from the rice plasma membrane after Cd or Cd and NO treatment. Sixty-six differentially expressed proteins were identified, of which, many function as transporters, ATPases, kinases, metabolic enzymes, phosphatases, and phospholipases. Among these, the abundance of phospholipase D (PLD) was altered substantially after the treatment of Cd or Cd and NO. Transient expression of the PLD fused with green fluorescent peptide (GFP) in rice protoplasts showed that the Cd and NO treatment promoted the accumulation of PLD in the plasma membrane. Addition of NO also enhanced Cd-induced PLD activity and the accumulation of phosphatidic acid (PA) produced through PLD activity. Meanwhile, NO elevated the activities of antioxidant enzymes and caused the accumulation of glutathione, both which function to reduce Cd-induced H2O2 accumulation. Taken together, we suggest that NO signaling is associated with the accumulation of antioxidant enzymes, glutathione and PA which increases cadmium tolerance in rice via the antioxidant defense system. C1 [Yang, Liming; Ji, Jianhui; Luo, Yuming] Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Hougze Lake, Huaian, Peoples R China. [Yang, Liming; Wang, Hui] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Yang, Liming] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA USA. [Harris-Shultz, Karen R.; Wang, Hongliang] ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA USA. [Abd-Allah, Elsayed F.] King Saud Univ, Fac Food & Agr Sci, Dept Plant Prod, Riyadh, Saudi Arabia. [Hu, Xiangyang] Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China. RP Luo, YM (reprint author), Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Hougze Lake, Huaian, Peoples R China.; Hu, XY (reprint author), Shanghai Univ, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China. EM yumingluo@163.com; huxiangyang@mail.kib.ac.cn FU National Science Foundation [30900871, 30971452, 31170256, 31400169]; Natural Science Foundation of Jiangsu Province [BK2011409, BK20140454]; Opening Foundation of the Jiansu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake [HZHL1002]; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture &Environment Protection [HSXT305]; Jiangsu Government Scholarship for Overseas Studies; Qinglan Project of Jiangsu Province; Deanship of Scientific Research at King Saud University [RG-1435-014] FX This work was supported by National Science Foundation grant of China (30900871, 30971452, 31170256, 31400169), Natural Science Foundation of Jiangsu Province (BK2011409, BK20140454), the Opening Foundation of the Jiansu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake (No.HZHL1002), Jiangsu Collaborative Innovation Center of Regional Modern Agriculture &Environment Protection (HSXT305), Jiangsu Government Scholarship for Overseas Studies and Qinglan Project of Jiangsu Province. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for funding this research (Research Group NO. RG-1435-014). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 55 TC 1 Z9 1 U1 18 U2 61 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 26 PY 2016 VL 7 AR 190 DI 10.3389/fpls.2016.00190 PG 18 WC Plant Sciences SC Plant Sciences GA DE7YR UT WOS:000370853500001 PM 26955374 ER PT J AU Gardner, IA Whittington, RJ Caraguel, CGB Hick, P Moody, NJG Corbeil, S Garver, KA Warg, JV Arzul, I Purcell, MK Crane, MS Waltzek, TB Olesen, NJ Lagno, AG AF Gardner, Ian A. Whittington, Richard J. Caraguel, Charles G. B. Hick, Paul Moody, Nicholas J. G. Corbeil, Serge Garver, Kyle A. Warg, Janet V. Arzul, Isabelle Purcell, Maureen K. Crane, Mark St J. Waltzek, Thomas B. Olesen, Niels J. Gallardo Lagno, Alicia TI Recommended reporting standards for test accuracy studies of infectious diseases of finfish, amphibians, molluscs and crustaceans: the STRADAS-aquatic checklist SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Reporting standards; Sensitivity; Specificity; Finfish; Amphibians; Molluscs; Crustaceans; STRADAS-paraTB; Diagnostic validation ID HEMORRHAGIC SEPTICEMIA VIRUS; HEMATOPOIETIC NECROSIS VIRUS; REAL-TIME PCR; SALMON-ANEMIA-VIRUS; DIAGNOSTIC-TEST ACCURACY; JUVENILE CHINOOK SALMON; LATENT-CLASS ANALYSIS; CHAIN-REACTION ASSAY; ONCORHYNCHUS-TSHAWYTSCHA; RENIBACTERIUM-SALMONINARUM AB Complete and transparent reporting of key elements of diagnostic accuracy studies for infectious diseases in cultured and wild aquatic animals benefits end-users of these tests, enabling the rational design of surveillance programs, the assessment of test results from clinical cases and comparisons of diagnostic test performance. Based on deficiencies in the Standards for Reporting of Diagnostic Accuracy (STARD) guidelines identified in a prior finfish study (Gardner et al. 2014), we adapted the Standards for Reporting of Animal Diagnostic Accuracy Studies-paratuberculosis (STRADAS-paraTB) checklist of 25 reporting items to increase their relevance to finfish, amphibians, molluscs, and crustaceans and provided examples and explanations for each item. The checklist, known as STRADAS-aquatic, was developed and refined by an expert group of 14 transdisciplinary scientists with experience in test evaluation studies using field and experimental samples, in operation of reference laboratories for aquatic animal pathogens, and in development of international aquatic animal health policy. The main changes to the STRADAS-paraTB checklist were to nomenclature related to the species, the addition of guidelines for experimental challenge studies, and the designation of some items as relevant only to experimental studies and ante-mortem tests. We believe that adoption of these guidelines will improve reporting of primary studies of test accuracy for aquatic animal diseases and facilitate assessment of their fitness-for-purpose. Given the importance of diagnostic tests to underpin the Sanitary and Phytosanitary agreement of the World Trade Organization, the principles outlined in this paper should be applied to other World Organisation for Animal Health (OIE)-relevant species. C1 [Gardner, Ian A.] Univ Prince Edward Isl, Atlantic Vet Coll, 550 Univ Ave, Charlottetown, PE C1A 4P3, Canada. [Whittington, Richard J.; Hick, Paul] Univ Sydney, Fac Vet Sci, Camden, NSW 2570, Australia. [Caraguel, Charles G. B.] Univ Adelaide, Sch Anim & Vet Sci, Roseworthy, SA 5371, Australia. [Moody, Nicholas J. G.; Corbeil, Serge; Crane, Mark St J.] CSIRO Australian Anim Hlth Lab, Geelong, Vic 3220, Australia. [Garver, Kyle A.] Fisheries & Oceans Canada, Pacific Biol Stn, Nanaimo, BC V9T 6N7, Canada. [Warg, Janet V.] USDA, Diagnost Virol Lab, Natl Vet Serv Labs, VS,APHIS, Ames, IA 50010 USA. [Arzul, Isabelle] IFREMER, SG2M, LGPMM, F-17390 La Tremblade, France. [Purcell, Maureen K.] US Geol Survey, Western Fisheries Res Ctr, Seattle, WA 98115 USA. [Waltzek, Thomas B.] Univ Florida, Dept Infect Dis & Pathol, Gainesville, FL 32611 USA. [Olesen, Niels J.] Tech Univ Denmark, Natl Vet Inst, Frederiksberg C, Denmark. [Gallardo Lagno, Alicia] Jefa Unidad Salud Anim Serv Nacl Pesca & Acuicult, Victoria 2832, Chile. RP Gardner, IA (reprint author), Univ Prince Edward Isl, Atlantic Vet Coll, 550 Univ Ave, Charlottetown, PE C1A 4P3, Canada. EM iagardner@upei.ca OI Purcell, Maureen/0000-0003-0154-8433; Arzul, Isabelle/0000-0001-5436-5927; Olesen, Jes/0000-0002-6712-2702 FU Canada Excellence Research Chairs Program FX This research was undertaken, in part, with funding from the Canada Excellence Research Chairs Program. We thank Emilie Laurin for technical assistance and an anonymous reviewer whose comments led to an improved manuscript. NR 53 TC 1 Z9 1 U1 2 U2 10 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD FEB 25 PY 2016 VL 118 IS 2 BP 91 EP 111 DI 10.3354/dao02947 PG 21 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA DF4GP UT WOS:000371306300001 PM 26912041 ER PT J AU Sawhney, N Crooks, C Chow, V Preston, JF St John, FJ AF Sawhney, Neha Crooks, Casey Chow, Virginia Preston, James F. St John, Franz J. TI Genomic and transcriptomic analysis of carbohydrate utilization by Paenibacillus sp JDR-2: systems for bioprocessing plant polysaccharides SO BMC GENOMICS LA English DT Article DE Paenibacillus sp JDR-2; Xylans; Soluble beta-glucan; Starch; Bioprocessing systems; Transcriptome; RNA sequencing; Biofuels and chemicals ID SP STRAIN JDR-2; UTILIZATION GENE-CLUSTER; LAYER HOMOLOGY DOMAINS; BACILLUS-SUBTILIS; CLOSTRIDIUM-THERMOCELLUM; GEOBACILLUS-STEAROTHERMOPHILUS; ALDOURONATE UTILIZATION; CELLULOSE UTILIZATION; THERMOTOGA-MARITIMA; XYLAN UTILIZATION AB Background: Polysaccharides comprising plant biomass are potential resources for conversion to fuels and chemicals. These polysaccharides include xylans derived from the hemicellulose of hardwoods and grasses, soluble beta-glucans from cereals and starch as the primary form of energy storage in plants. Paenibacillus sp. JDR-2 (Pjdr2) has evolved a system for bioprocessing xylans. The central component of this xylan utilization system is a multimodular glycoside hydrolase family 10 (GH10) endoxylanase with carbohydrate binding modules (CBM) for binding xylans and surface layer homology (SLH) domains for cell surface anchoring. These attributes allow efficient utilization of xylans by generating oligosaccharides proximal to the cell surface for rapid assimilation. Coordinate expression of genes in response to growth on xylans has identified regulons contributing to depolymerization, importation of oligosaccharides and intracellular processing to generate xylose as well as arabinose and methylglucuronate. The genome of Pjdr2 encodes several other putative surface anchored multimodular enzymes including those for utilization of beta-1,3/1,4 mixed linkage soluble glucan and starch. Results: To further define polysaccharide utilization systems in Pjdr2, its transcriptome has been determined by RNA sequencing following growth on barley-derived soluble beta-glucan, starch, cellobiose, maltose, glucose, xylose and arabinose. The putative function of genes encoding transcriptional regulators, ABC transporters, and glycoside hydrolases belonging to the corresponding substrate responsive regulon were deduced by their coordinate expression and locations in the genome. These results are compared to observations from the previously defined xylan utilization systems in Pjdr2. The findings from this study show that Pjdr2 efficiently utilizes these glucans in a manner similar to xylans. From transcriptomic and genomic analyses we infer a common strategy evolved by Pjdr2 for efficient bioprocessing of polysaccharides. Conclusions: The barley beta-glucan and starch utilization systems in Pjdr2 include extracellular glycoside hydrolases bearing CBM and SLH domains for depolymerization of these polysaccharides. Overlapping regulation observed during growth on these polysaccharides suggests they are preferentially utilized in the order of starch before xylan before barley beta-glucan. These systems defined in Pjdr2 may serve as a paradigm for developing biocatalysts for efficient bioprocessing of plant biomass to targeted biofuels and chemicals. C1 [Sawhney, Neha; Chow, Virginia; Preston, James F.] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. [Crooks, Casey; St John, Franz J.] US Forest Serv, Inst Microbial & Biochem Technol, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Preston, JF (reprint author), Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA.; St John, FJ (reprint author), US Forest Serv, Inst Microbial & Biochem Technol, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM jpreston@ufl.edu; fjstjohn@gmail.com FU Office of Science of US Department of Energy [DE-AC02-05CH11231]; Biomass Research & Development Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2011-10006-30358]; Florida Energy Systems Consortium, State University System of FL [00077818] FX We appreciate the assistance provided by Dr. L. O. Ingram, Dr. K. T. Shanmugam, and John D. Rice all from the Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, for useful advice. We also thank Dr. Daniel Cullen for his expertise and helpful discussions regarding transcriptome studies and Diane Dietrich for professional support, both from the USDA, US Forest Service, Forest Products Laboratory, Madison, WI. We would like to thank Christa Pennacchio for managing the RNA sequencing project and also the other members of the Joint Genome Institute, Walnut Creek, CA, for conducting the RNA sequencing work which was supported by the Office of Science of the US Department of Energy under Contract No. DE-AC02-05CH11231. This research was supported by Biomass Research & Development Initiative Competitive Grant No. 2011-10006-30358 from the USDA National Institute of Food and Agriculture, and by Florida Energy Systems Consortium, State University System of FL, Project No. 00077818. NR 51 TC 1 Z9 1 U1 1 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 24 PY 2016 VL 17 AR 131 DI 10.1186/s12864-016-2436-5 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DF8FS UT WOS:000371593400002 PM 26912334 ER PT J AU Dennis, B Kemp, WP AF Dennis, Brian Kemp, William P. TI How Hives Collapse: Allee Effects, Ecological Resilience, and the Honey Bee SO PLOS ONE LA English DT Article ID POPULATION-DYNAMICS; DISORDER; MODEL; COLONIES; STATES; SCALE AB We construct a mathematical model to quantify the loss of resilience in collapsing honey bee colonies due to the presence of a strong Allee effect. In the model, recruitment and mortality of adult bees have substantial social components, with recruitment enhanced and mortality reduced by additional adult bee numbers. The result is an Allee effect, a net per-individual rate of hive increase that increases as a function of adult bee numbers. The Allee effect creates a critical minimum size in adult bee numbers, below which mortality is greater than recruitment, with ensuing loss of viability of the hive. Under ordinary and favorable environmental circumstances, the critical size is low, and hives remain large, sending off viably-sized swarms (naturally or through beekeeping management) when hive numbers approach an upper stable equilibrium size (carrying capacity). However, both the lower critical size and the upper stable size depend on many parameters related to demographic rates and their enhancement by bee sociality. Any environmental factors that increase mortality, decrease recruitment, or interfere with the social moderation of these rates has the effect of exacerbating the Allee effect by increasing the lower critical size and substantially decreasing the upper stable size. As well, the basin of attraction to the upper stable size, defined by the model potential function, becomes narrower and shallower, indicating the loss of resilience as the hive becomes subjected to increased risk of falling below the critical size. Environmental effects of greater severity can cause the two equilibria to merge and the basin of attraction to the upper stable size to disappear, resulting in collapse of the hive from any initial size. The model suggests that multiple proximate causes, among them pesticides, mites, pathogens, and climate change, working singly or in combinations, could trigger hive collapse. C1 [Dennis, Brian] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Dennis, Brian] Univ Idaho, Dept Stat Sci, Moscow, ID 83844 USA. [Kemp, William P.] ARS, USDA, Red River Valley Agr Res Ctr, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. RP Dennis, B (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA.; Dennis, B (reprint author), Univ Idaho, Dept Stat Sci, Moscow, ID 83844 USA. EM brian@uidaho.edu FU US Department of Agriculture FX This work was supported by US Department of Agriculture (http://www.ars.usda.gov/main/site_main.htm?modecode=30-00-00-00 58-5442-2-327) to BD. NR 54 TC 3 Z9 3 U1 27 U2 77 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 24 PY 2016 VL 11 IS 2 AR e0150055 DI 10.1371/journal.pone.0150055 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF2IA UT WOS:000371164700059 PM 26910061 ER PT J AU Liu, HQ Dong, CL Zhao, TC Han, JC Wang, TL Wen, XZ Huang, Q AF Liu, Huiqin Dong, Chunling Zhao, Tingchang Han, Jucai Wang, Tieling Wen, Xiangzhen Huang, Qi TI Functional Analysis of the Ferric Uptake Regulator Gene fur in Xanthomonas vesicatoria SO PLOS ONE LA English DT Article ID QUORUM-SENSING SIGNAL; ORYZAE PV. ORYZAE; PSEUDOMONAS-AERUGINOSA; ESCHERICHIA-COLI; MOLECULAR ANALYSIS; SWARMING MOTILITY; VIRULENCE FACTORS; SMALL RNAS; IRON; CAMPESTRIS AB Iron is essential for the growth and survival of many organisms. Intracellular iron homeostasis must be maintained for cell survival and protection against iron toxicity. The ferric uptake regulator protein (Fur) regulates the high-affinity ferric uptake system in many bacteria. To investigate the function of the fur gene in Xanthomonas vesicatoria (Xv), we generated a fur mutant strain, fur-m, by site-directed mutagenesis. Whereas siderophore production increased in the Xv fur mutant, extracellular polysaccharide production, biofilm formation, swimming ability and quorum sensing signals were all significantly decreased. The fur mutant also had significantly reduced virulence in tomato leaves. The above-mentioned phenotypes significantly recovered when the Xv fur mutation allele was complemented with a wild-type fur gene. Thus, Fur either negatively or positively regulates multiple important physiological functions in Xv. C1 [Liu, Huiqin] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Liu, Huiqin; Dong, Chunling; Zhao, Tingchang; Wang, Tieling] Tianjin Agr Univ, Coll Hort & Landscape, Tianjin, Peoples R China. [Liu, Huiqin; Han, Jucai] Shanxi Agr Univ, Agron Coll, Taigu, Shanxi, Peoples R China. [Wen, Xiangzhen] Shanxi Agr Univ, Hort Coll, Taigu, Shanxi, Peoples R China. [Huang, Qi] ARS, Floral & Nursery Plants Res Unit, USDA, Beltsville, MD USA. RP Zhao, TC (reprint author), Tianjin Agr Univ, Coll Hort & Landscape, Tianjin, Peoples R China. EM tzhaotgcg@163.com FU earmarked fund for Modern Agro-industry Technology Research System [CARS-26]; Special Fund for Agro-scientific Research in the Public Interest [201003066] FX This research was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-26) and Special Fund for Agro-scientific Research in the Public Interest (201003066). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 0 Z9 0 U1 2 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 24 PY 2016 VL 11 IS 2 AR e0149280 DI 10.1371/journal.pone.0149280 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF2IA UT WOS:000371164700028 PM 26910324 ER PT J AU Figueroa, M Upadhyaya, NM Sperschneider, J Park, RF Szabo, LJ Steffenson, B Ellis, JG Dodds, PN AF Figueroa, Melania Upadhyaya, Narayana M. Sperschneider, Jana Park, Robert F. Szabo, Les J. Steffenson, Brian Ellis, Jeff G. Dodds, Peter N. TI Changing the Game: Using Integrative Genomics to Probe Virulence Mechanisms of the Stem Rust Pathogen Puccinia graminis f. sp tritici SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE Puccinia; effectors; resistance; stem rust; virulence; avirulence; wheat; barley ID MULTIPLE FUNGAL PATHOGENS; FLAX-RUST; DISEASE-RESISTANCE; AVIRULENCE GENES; LEAF RUST; CANDIDATE EFFECTORS; CONFERS RESISTANCE; SECRETED PROTEINS; PLANT PATHOLOGY; RACE GROUP AB The recent resurgence of wheat stem rust caused by new virulent races of Puccinia graminis f. sp. tritici (Pgt) poses a threat to food security. These concerns have catalyzed an extensive global effort toward controlling this disease. Substantial research and breeding programs target the identification and introduction of new stem rust resistance (Sr) genes in cultivars for genetic protection against the disease. Such resistance genes typically encode immune receptor proteins that recognize specific components of the pathogen, known as avirulence (Avr) proteins. A significant drawback to deploying cultivars with single Sr genes is that they are often overcome by evolution of the pathogen to escape recognition through alterations in Avr genes. Thus, a key element in achieving durable rust control is the deployment of multiple effective Sr genes in combination, either through conventional breeding or transgenic approaches, to minimize the risk of resistance breakdown. In this situation, evolution of pathogen virulence would require changes in multiple Avr genes in order to bypass recognition. However, choosing the optimal Sr gene combinations to deploy is a challenge that requires detailed knowledge of the pathogen Avr genes with which they interact and the virulence phenotypes of Pgt existing in nature. Identifying specific Avr genes from Pgt will provide screening tools to enhance pathogen virulence monitoring, assess heterozygosity and propensity for mutation in pathogen populations, and confirm individual Sr gene functions in crop varieties carrying multiple effective resistance genes. Toward this goal, much progress has been made in assembling a high quality reference genome sequence for Pgt, as well as a Pan genome encompassing variation between multiple field isolates with diverse virulence spectra. In turn this has allowed prediction of Pgt effector gene candidates based on known features of Avr genes in other plant pathogens, including the related flax rust fungus. Upregulation of gene expression in haustoria and evidence for diversifying selection are two useful parameters to identify candidate Avr genes. Recently, we have also applied machine learning approaches to agnostically predict candidate effectors. Here, we review progress in stem rust pathogenomics and approaches currently underway to identify Avr genes recognized by wheat Sr genes. C1 [Figueroa, Melania; Szabo, Les J.; Steffenson, Brian] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Figueroa, Melania; Szabo, Les J.; Steffenson, Brian] Univ Minnesota, Stakman Borlaug Ctr Sustainable Plant Hlth, St Paul, MN USA. [Upadhyaya, Narayana M.; Ellis, Jeff G.; Dodds, Peter N.] CSIRO, Agr, Canberra, ACT, Australia. [Sperschneider, Jana] CSIRO, Agr, Ctr Environm & Life Sci, Perth, WA, Australia. [Park, Robert F.] Univ Sydney, Plant Breeding Inst, Fac Agr & Environm, Narellan, NSW, Australia. [Szabo, Les J.] USDA ARS, Cereal Dis Lab, St Paul, MN USA. RP Dodds, PN (reprint author), CSIRO, Agr, Canberra, ACT, Australia. EM peter.dodds@csiro.au RI Dodds, Peter/D-1181-2009; Ellis, Jeffrey/A-1999-2010; OI Dodds, Peter/0000-0003-0620-5923; Sperschneider, Jana/0000-0002-9385-8588 FU Two Blades Foundation; Grains Research and Development Corporation [US00063]; University of Minnesota; Durable Rust Resistance in Wheat Project; Bill & Melinda Gates Foundation; UK Department for International Development, LiebermanOkinow Endowment at the University of Minnesota; USDA-ARS FX Work in the authors' laboratories has been supported by funds provided through the Two Blades Foundation, the Grains Research and Development Corporation (US00063), start-up funds provided by the University of Minnesota, the Durable Rust Resistance in Wheat Project administered by Cornell University and supported by the Bill & Melinda Gates Foundation and the UK Department for International Development, LiebermanOkinow Endowment at the University of Minnesota, and USDA-ARS. NR 106 TC 2 Z9 2 U1 10 U2 35 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 24 PY 2016 VL 7 AR 205 DI 10.3389/fpls.2016.00205 PG 10 WC Plant Sciences SC Plant Sciences GA DE6RX UT WOS:000370762400001 PM 26941766 ER PT J AU Schetters, T Bishop, R Crampton, M Kopacek, P Lew-Tabor, A Maritz-Olivier, C Miller, R Mosqueda, J Patarroyo, J Rodriguez-Valle, M Scoles, GA de la Fuente, J AF Schetters, Theo Bishop, Richard Crampton, Michael Kopacek, Petr Lew-Tabor, Alicja Maritz-Olivier, Christine Miller, Robert Mosqueda, Juan Patarroyo, Joaquin Rodriguez-Valle, Manuel Scoles, Glen A. de la Fuente, Jose TI Cattle tick vaccine researchers join forces in CATVAC SO PARASITES & VECTORS LA English DT Article DE CATVAC; Vaccine; Cattle; Tick; Rhipicephalus microplus ID RHIPICEPHALUS BOOPHILUS MICROPLUS; INFESTATIONS; IMMUNIZATION; AQUAPORIN; ANNULATUS; ANTIGEN AB A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14-15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC). C1 [Schetters, Theo] ProtAct R&D, Cuijk, Netherlands. [Schetters, Theo] ClinVet Int, Bloemfontein, South Africa. [Bishop, Richard] ILRI, Tick Unit, Nairobi, Kenya. [Crampton, Michael] CSIR, ZA-0001 Pretoria, Nairobi, South Africa. [Kopacek, Petr] Acad Sci Czech Republic, Inst Parasitol, Ctr Biol, Branisovska 31, CR-37005 Ceske Budejovice, Czech Republic. [Lew-Tabor, Alicja; Rodriguez-Valle, Manuel] Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld, Australia. [Lew-Tabor, Alicja] Murdoch Univ, Ctr Comparat Gen, Perth, WA, Australia. [Maritz-Olivier, Christine] Univ Pretoria, Fac Nat & Agr Sci, Dept Genet, Genom Res Inst, ZA-0002 Pretoria, South Africa. [Miller, Robert] ARS, Cattle Fever Tick Res Lab, USDA, Edinburg, TX USA. [Mosqueda, Juan] Univ Autonoma Queretaro, Fac Ciencias Nat, Queretaro, Queretaro, Mexico. [Patarroyo, Joaquin] Univ Fed Vicosa, BIOAGRO DVT, Vicosa, MG, Brazil. [Scoles, Glen A.] Washington State Univ, ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [de la Fuente, Jose] IREC CSIC UCLM JCCM, Inst Invest Recursos Cineget, SaBio, Ciudad Real, Spain. [de la Fuente, Jose] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA. RP Schetters, T (reprint author), ProtAct R&D, Cuijk, Netherlands.; Schetters, T (reprint author), ClinVet Int, Bloemfontein, South Africa. EM th.schetters@protactivity.com RI Kopacek, Petr/G-8414-2014; Lew-Tabor, Ala/A-5286-2010 OI Lew-Tabor, Ala/0000-0001-7147-1078 FU Bill & Melinda Gates Foundation FX This meeting was supported by the Bill & Melinda Gates Foundation. Special thanks to Emily Moran of the Gates foundation for helping to organize the meeting. The authors wish to express their gratitude to Dr. Peter Willadsen, Dr. Josephus Fourie (ClinVet International, Bloemfontein, South Africa) and Dr. Nick Juleff (Gates Foundation) for continuous support and advice. The opinions and assertions contained herein are those of the authors. NR 23 TC 4 Z9 4 U1 0 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 24 PY 2016 VL 9 AR 105 DI 10.1186/s13071-016-1386-8 PG 7 WC Parasitology SC Parasitology GA DE5PB UT WOS:000370682400001 PM 26911668 ER PT J AU Adetona, O Reinhardt, TE Domitrovich, J Broyles, G Adetona, AM Kleinman, MT Ottmar, RD Naeher, LP AF Adetona, Olorunfemi Reinhardt, Timothy E. Domitrovich, Joe Broyles, George Adetona, Anna M. Kleinman, Michael T. Ottmar, Roger D. Naeher, Luke P. TI Review of the health effects of wildland fire smoke on wildland firefighters and the public SO INHALATION TOXICOLOGY LA English DT Review DE Cardiovascular effects; respiratory effects; toxicity mechanism; wildfire; wildland firefighter ID INDOOR AIR-POLLUTION; INHALED WOOD SMOKE; FINE-PARTICLE EMISSIONS; SOUTHERN CALIFORNIA WILDFIRES; OBSTRUCTIVE PULMONARY-DISEASE; SHORT-TERM EXPOSURE; LOW-BIRTH-WEIGHT; CARDIORESPIRATORY HOSPITAL ADMISSIONS; SYSTEMIC INFLAMMATORY RESPONSE; ACUTE RESPIRATORY-INFECTIONS AB Each year, the general public and wildland firefighters in the US are exposed to smoke from wildland fires. As part of an effort to characterize health risks of breathing this smoke, a review of the literature was conducted using five major databases, including PubMed and MEDLINE Web of Knowledge, to identify smoke components that present the highest hazard potential, the mechanisms of toxicity, review epidemiological studies for health effects and identify the current gap in knowledge on the health impacts of wildland fire smoke exposure. Respiratory events measured in time series studies as incidences of disease-caused mortality, hospital admissions, emergency room visits and symptoms in asthma and chronic obstructive pulmonary disease patients are the health effects that are most commonly associated with community level exposure to wildland fire smoke. A few recent studies have also determined associations between acute wildland fire smoke exposure and cardiovascular health end-points. These cardiopulmonary effects were mostly observed in association with ambient air concentrations of fine particulate matter (PM2.5). However, research on the health effects of this mixture is currently limited. The health effects of acute exposures beyond susceptible populations and the effects of chronic exposures experienced by the wildland firefighter are largely unknown. Longitudinal studies of wildland firefighters during and/or after the firefighting career could help elucidate some of the unknown health impacts of cumulative exposure to wildland fire smoke, establish occupational exposure limits and help determine the types of exposure controls that may be applicable to the occupation. C1 [Adetona, Olorunfemi; Adetona, Anna M.; Naeher, Luke P.] Univ Georgia, Dept Environm Hlth Sci, Coll Publ Hlth, Environm Hlth Sci Bldg, Athens, GA 30602 USA. [Adetona, Olorunfemi] Ohio State Univ, Coll Publ Hlth, Div Environm Hlth Sci, Columbus, OH 43210 USA. [Reinhardt, Timothy E.] AMEC Foster Wheeler Environm & Infrastruct Inc, Seattle, WA USA. [Domitrovich, Joe] US Forest Serv, USDA, Missoula Technol & Dev Ctr, Missoula, MT USA. [Broyles, George] San Dimas Technol & Dev Ctr, SDA Forest Serv, San Dimas, CA USA. [Kleinman, Michael T.] Univ Calif Irvine, Ctr Occupat & Environm Hlth, Irvine, CA USA. [Ottmar, Roger D.] US Forest Serv, USDA, Pacific NW Res Stn, Seattle, WA USA. RP Naeher, LP (reprint author), Univ Georgia, Dept Environm Hlth Sci, Coll Publ Hlth, Environm Hlth Sci Bldg, Athens, GA 30602 USA. EM LNaeher@uga.edu RI Adetona, Olorunfemi/A-2434-2016 FU Joint Fire Science Program [13-1-02-14]; Pacific Northwest Research station of the United States Forest Service FX The authors acknowledge funding from the Joint Fire Science Program (Project # 13-1-02-14) with additional support from the Pacific Northwest Research station of the United States Forest Service. The manuscript reflects solely the opinion of the authors and not of the funding source. The authors declare that there are no conflicts of interest. NR 287 TC 3 Z9 3 U1 18 U2 46 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0895-8378 EI 1091-7691 J9 INHAL TOXICOL JI Inhal. Toxicol. PD FEB 23 PY 2016 VL 28 IS 3 BP 95 EP 139 DI 10.3109/08958378.2016.1145771 PG 45 WC Toxicology SC Toxicology GA DG1EI UT WOS:000371808000001 PM 26915822 ER PT J AU Blackwell, M Kurtzman, CP AF Blackwell, Meredith Kurtzman, Cletus P. TI Social wasps promote social behavior in Saccharomyces spp. SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Editorial Material ID YEASTS; CEREVISIAE; PARADOXUS; WINE; POPULATIONS; GUT C1 [Blackwell, Meredith] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Blackwell, Meredith] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. [Kurtzman, Cletus P.] USDA ARS, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. RP Blackwell, M (reprint author), Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA.; Blackwell, M (reprint author), Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. EM mblackwell@lsu.edu NR 21 TC 0 Z9 0 U1 3 U2 9 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD FEB 23 PY 2016 VL 113 IS 8 BP 1971 EP 1973 DI 10.1073/pnas.1600173113 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DE4SK UT WOS:000370620300031 PM 26884169 ER PT J AU Foster, J Luo, B Nakata, PA AF Foster, Justin Luo, Bin Nakata, Paul A. TI An Oxalyl-CoA Dependent Pathway of Oxalate Catabolism Plays a Role in Regulating Calcium Oxalate Crystal Accumulation and Defending against Oxalate-Secreting Phytopathogens in Medicago truncatula SO PLOS ONE LA English DT Article ID TRANSIENT EXPRESSION SYSTEM; SCLEROTINIA-SCLEROTIORUM; SACCHAROMYCES-CEREVISIAE; ARABIDOPSIS-THALIANA; ACID; PLANTS; PROTEINS; MUTANTS; GERMIN; TRANSFORMATION AB Considering the widespread occurrence of oxalate in nature and its broad impact on a host of organisms, it is surprising that so little is known about the turnover of this important acid. In plants, oxalate oxidase is the most well studied enzyme capable of degrading oxalate, but not all plants possess this activity. Recently, an Acyl Activating Enzyme 3 (AAE3), encoding an oxalyl-CoA synthetase, was identified in Arabidopsis. AAE3 has been proposed to catalyze the first step in an alternative pathway of oxalate degradation. Whether this enzyme and proposed pathway is important to other plants is unknown. Here, we identify the Medicago truncatula AAE3 (MtAAE3) and show that it encodes an oxalyl-CoA synthetase activity exhibiting high activity against oxalate with a Km = 81 +/- 9 mu M and Vmax = 19 +/- 0.9 mu moles min(-1)mg protein(-1). GFP-MtAAE3 localization suggested that this enzyme functions within the cytosol of the cell. Mtaae3 knock-down line showed a reduction in its ability to degrade oxalate into CO2. This reduction in the capacity to degrade oxalate resulted in the accumulation of druse crystals of calcium oxalate in the Mtaae3 knock-down line and an increased susceptibility to oxalate-secreting phytopathogens such as Sclerotinia sclerotiorum. Taken together, these results suggest that AAE3 dependent turnover of oxalate is important to different plants and functions in the regulation of tissue calcium oxalate crystal accumulation and in defense against oxalate-secreting phytopathogens. C1 [Foster, Justin; Luo, Bin; Nakata, Paul A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Nakata, PA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM paul.nakata@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service [58-3092-5-001] FX This work was supported by the U.S. Department of Agriculture, Agricultural Research Service, under Cooperative agreement number 58-3092-5-001. NR 53 TC 4 Z9 4 U1 1 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 22 PY 2016 VL 11 IS 2 AR e0149850 DI 10.1371/journal.pone.0149850 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3VL UT WOS:000371276100164 PM 26900946 ER PT J AU Sainju, UM AF Sainju, Upendra M. TI A Global Meta-Analysis on the Impact of Management Practices on Net Global Warming Potential and Greenhouse Gas Intensity from Cropland Soils SO PLOS ONE LA English DT Article ID IRRIGATED CROPPING SYSTEMS; NITROUS-OXIDE EMISSIONS; CARBON SEQUESTRATION; LONG-TERM; NORTHEASTERN COLORADO; TILLAGE; AGRICULTURE; FERTILIZATION; MITIGATION; ROTATION AB Management practices, such as tillage, crop rotation, and N fertilization, may affect net global warming potential (GWP) and greenhouse gas intensity (GHGI), but their global impact on cropland soils under different soil and climatic conditions need further evaluation. Available global data from 57 experiments and 225 treatments were evaluated for individual and combined effects of tillage, cropping systems, and N fertilization rates on GWP and GHGI which accounted for CO2 equivalents from N2O and CH4 emissions with or without equivalents from soil C sequestration rate (Delta SOC), farm operations, and N fertilization. The GWP and GHGI were 66 to 71% lower with no-till than conventional till and 168 to 215% lower with perennial than annual cropping systems, but 41 to 46% greater with crop rotation than monocroppping. With no-till vs. conventional till, GWP and GHGI were 2.6- to 7.4-fold lower when partial than full accounting of all sources and sinks of greenhouse gases (GHGs) were considered. With 100 kg N ha(-1), GWP and GHGI were 3.2 to 11.4 times greater with partial than full accounting. Both GWP and GHGI increased curvilinearly with increased N fertilization rate. Net GWP and GHGI were 70 to 87% lower in the improved combined management that included no-till, crop rotation/perennial crop, and reduced N rate than the traditional combined management that included conventional till, monocopping/annual crop, and recommended N rate. An alternative soil respiration method, which replaces Delta SOC by soil respiration and crop residue returned to soil in the previous year, similarly reduced GWP and GHGI by 133 to 158% in the improved vs. the traditional combined management. Changes in GWP and GHGI due to improved vs. traditional management varied with the duration of the experiment and inclusion of soil and climatic factors in multiple linear regressions improved their relationships. Improved management practices reduced GWP and GHGI compared with traditional management practices and combined management practices were even more effective than individual management practices in reducing net GHG emissions from cropland soils. Partial accounting overestimated GWP and GHGI values as sinks or sources of net GHGs compared with full accounting when evaluating the effect of management practices. C1 [Sainju, Upendra M.] ARS, USDA, Northern Plain Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. RP Sainju, UM (reprint author), ARS, USDA, Northern Plain Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov NR 55 TC 1 Z9 1 U1 19 U2 56 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 22 PY 2016 VL 11 IS 2 AR e0148527 DI 10.1371/journal.pone.0148527 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3VL UT WOS:000371276100018 PM 26901827 ER PT J AU Sharma, VK Kudva, IT Bearson, BL Stasko, JA AF Sharma, Vijay K. Kudva, Indira T. Bearson, Bradley L. Stasko, Judith A. TI Contributions of EspA Filaments and Curli Fimbriae in Cellular Adherence and Biofilm Formation of Enterohemorrhagic Escherichia coli O157:H7 SO PLOS ONE LA English DT Article ID ENTEROCYTE EFFACEMENT GENES; O157-H7 REQUIRES INTIMIN; III SECRETION SYSTEM; BUNDLE-FORMING PILI; EPITHELIAL-CELLS; TRANSCRIPTIONAL REGULATION; PROTEIN TRANSLOCATION; PATHOGENICITY ISLAND; ABIOTIC SURFACES; TERMINAL RECTUM AB In Escherichia coli O157:H7 (O157), the filamentous structure of the type III secretion system is produced from the polymerization of the EspA protein. EspA filaments are essential for O157 adherence to epithelial cells. In previous studies, we demonstrated that O157 hha deletion mutants showed increased adherence to HEp-2 cells and produced abundant biofilms. Transcriptional analysis revealed increased expression of espA as well as the csgA gene, which encodes curli fimbriae that are essential for biofilm formation. In the present study, we constructed hha espA, hha csgA, and hha csgA espA deletion mutants to determine the relative importance of EspA and CsgA in O157 adherence to HEp-2 cells and biofilm formation. In vitro adherence assays, conducted at 37 degrees C in a tissue culture medium containing 0.1% glucose, showed that HEp-2 cell adherence required EspA because hha espA and hha csgA espA mutants adhered to HEp-2 cells at higher levels only when complemented with an espA-expressing plasmid. Biofilm assays performed at 28 degrees C in a medium lacking glucose showed dependency of biofilm formation on CsgA; however EspA was not produced under these conditions. Despite production of detectable levels of EspA at 37 degrees C in media supplemented with 0.1% glucose, the biofilm formation occurred independent of EspA. These results indicate dependency of O157 adherence to epithelial cells on EspA filaments, while CsgA promoted biofilm formation under conditions mimicking those found in the environment (low temperature with nutrient limitations) and in the digestive tract of an host animal (higher temperature and low levels of glucose). C1 [Sharma, Vijay K.; Kudva, Indira T.] USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Bearson, Bradley L.] USDA ARS, Agroecosyst Management Res Unit, Natl Lab Agr & Environm, Ames, IA USA. [Stasko, Judith A.] USDA ARS, Microscopy Serv Lab, Natl Anim Dis Ctr, Ames, IA USA. RP Sharma, VK (reprint author), USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA USA. EM vijay.sharma@ars.usda.gov NR 74 TC 1 Z9 1 U1 3 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 22 PY 2016 VL 11 IS 2 AR e0149745 DI 10.1371/journal.pone.0149745 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3VL UT WOS:000371276100140 PM 26900701 ER PT J AU Abdurakhmonov, IY Ayubov, MS Ubaydullaeva, KA Buriev, ZT Shermatov, SE Ruziboev, HS Shapulatov, UM Saha, S Ulloa, M Yu, JZ Percy, RG Devor, EJ Sharma, GC Sripathi, VR Kumpatla, SP van der Krol, A Kater, HD Khamidov, K Salikhov, SI Jenkins, JN Abdukarimov, A Pepper, AE AF Abdurakhmonov, Ibrokhim Y. Ayubov, Mirzakamol S. Ubaydullaeva, Khurshida A. Buriev, Zabardast T. Shermatov, Shukhrat E. Ruziboev, Haydarali S. Shapulatov, Umid M. Saha, Sukumar Ulloa, Mauricio Yu, John Z. Percy, Richard G. Devor, Eric J. Sharma, Govind C. Sripathi, Venkateswara R. Kumpatla, Siva P. van der Krol, Alexander Kater, Hake D. Khamidov, Khakimdjan Salikhov, Shavkat I. Jenkins, Johnie N. Abdukarimov, Abdusattor Pepper, Alan E. TI RNA Interference for Functional Genomics and Improvement of Cotton (Gossypium sp.) SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE antisense; cotton pest control; disease resistance; fiber quality; Gossypium; gene silencing ID MYB TRANSCRIPTION FACTOR; FIBER CELL INITIATION; HIRSUTUM L.; GENE-EXPRESSION; SYNTHASE GENE; SOMATIC-EMBRYOGENESIS; VERTICILLIUM-DAHLIAE; VACUOLAR INVERTASE; CULTIVATED COTTON; QUALITY TRAITS AB RNA interference (RNAi), is a powerful new technology in the discovery of genetic sequence functions, and has become a valuable tool for functional genomics of cotton (Gossypium sp.). The rapid adoption of RNAi has replaced previous antisense technology. RNAi has aided in the discovery of function and biological roles of many key cotton genes involved in fiber development, fertility and somatic embryogenesis, resistance to important biotic and abiotic stresses, and oil and seed quality improvements as well as the key agronomic traits including yield and maturity. Here, we have comparatively reviewed seminal research efforts in previously used antisense approaches and currently applied breakthrough RNAi studies in cotton, analyzing developed RNAi methodologies, achievements, limitations, and future needs in functional characterizations of cotton genes. We also highlighted needed efforts in the development of RNAi-based cotton cultivars, and their safety and risk assessment, small and large-scale field trials, and commercialization. C1 [Abdurakhmonov, Ibrokhim Y.; Ayubov, Mirzakamol S.; Ubaydullaeva, Khurshida A.; Buriev, Zabardast T.; Shermatov, Shukhrat E.; Ruziboev, Haydarali S.; Shapulatov, Umid M.; Khamidov, Khakimdjan; Salikhov, Shavkat I.; Abdukarimov, Abdusattor] Acad Sci Uzbek, Ctr Genom & Bioinformat Struct & Funct Genom, Minist Agr & Water Resources Republ Uzbekistan, Kibray, Uzbekistan. [Abdurakhmonov, Ibrokhim Y.; Ayubov, Mirzakamol S.; Ubaydullaeva, Khurshida A.; Buriev, Zabardast T.; Shermatov, Shukhrat E.; Ruziboev, Haydarali S.; Shapulatov, Umid M.; Khamidov, Khakimdjan; Salikhov, Shavkat I.; Abdukarimov, Abdusattor] Uzpakhtasanoat Assoc, Kibray, Uzbekistan. [Shapulatov, Umid M.; van der Krol, Alexander] Wageningen Univ, Lab Plant Physiol, NL-6700 AP Wageningen, Netherlands. [Saha, Sukumar; Jenkins, Johnie N.] USDA ARS, Crop Sci Res Lab, Starkville, MS USA. [Ulloa, Mauricio] USDA ARS, Plant Stress & Germplasm Dev Res, Lubbock, TX USA. [Yu, John Z.; Percy, Richard G.] USDA ARS, Crop Germplasm Res Unit, College Stn, TX USA. [Devor, Eric J.] Univ Iowa, Coll Med, Dept Obstet & Gynecol, Iowa City, IA 52242 USA. [Sharma, Govind C.; Sripathi, Venkateswara R.] Alabama A&M Univ, Dept Biol & Environm Sci, Normal, AL 35762 USA. [Kumpatla, Siva P.] Dow AgroSci LLC, Indianapolis, IN USA. [Kater, Hake D.] Agr & Environm Res, Cary, NC USA. [Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. RP Abdurakhmonov, IY (reprint author), Acad Sci Uzbek, Ctr Genom & Bioinformat Struct & Funct Genom, Minist Agr & Water Resources Republ Uzbekistan, Kibray, Uzbekistan.; Abdurakhmonov, IY (reprint author), Uzpakhtasanoat Assoc, Kibray, Uzbekistan. EM genomics@uzsci.net RI Abdurakhmonov, Ibrokhim/F-5588-2015 OI Abdurakhmonov, Ibrokhim/0000-0001-9563-0686 FU Academy of Sciences of Uzbekistan; Committee for Coordination Science and Technology Development of Uzbekistan [FA-F5-T030, FA-A6-T081, FA-A6-T085, I-2015-6-15/2, I5-FQ-0-89-870]; Office of International Research Programs (OIRP) of the United States Department of Agriculture (USDA) Agricultural-Research Service (ARS); U.S. Civilian Research and Development Foundation (CRDF) [P121, P121B, UZB-TA-2992] FX We thank Academy of Sciences of Uzbekistan, and Committee for Coordination Science and Technology Development of Uzbekistan for basic science (FA-F5-T030), and several applied (FA-A6-T081 and FA-A6-T085), and innovation (I-2015-6-15/2 and 15-FQ-0-89-870) research grants. We greatly acknowledge the Office of International Research Programs (OIRP) of the United States Department of Agriculture (USDA) Agricultural-Research Service (ARS) and U.S. Civilian Research and Development Foundation (CRDF) for international cooperative grants P121, P121B, and UZB-TA-2992, which were devoted to cotton RNA interference research. NR 134 TC 0 Z9 0 U1 8 U2 33 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 22 PY 2016 VL 7 AR 202 DI 10.3389/fpls.2016.00202 PG 17 WC Plant Sciences SC Plant Sciences GA DE3RO UT WOS:000370547900001 PM 26941765 ER PT J AU Desgroux, A L'Anthoene, V Roux-Duparque, M Riviere, JP Aubert, G Tayeh, N Moussart, A Mangin, P Vetel, P Piriou, C McGee, RJ Coyne, CJ Burstin, J Baranger, A Manzanares-Dauleux, M Bourion, V Pilet-Nayel, ML AF Desgroux, Aurore L'Anthoene, Virginie Roux-Duparque, Martine Riviere, Jean-Philippe Aubert, Gregoire Tayeh, Nadim Moussart, Anne Mangin, Pierre Vetel, Pierrick Piriou, Christophe McGee, Rebecca J. Coyne, Clarice J. Burstin, Judith Baranger, Alain Manzanares-Dauleux, Maria Bourion, Virginie Pilet-Nayel, Marie-Laure TI Genome-wide association mapping of partial resistance to Aphanomyces euteiches in pea SO BMC GENOMICS LA English DT Article DE Root rot; Plant disease resistance; GWAS; Pea (Pisum sativum); Quantitative trait loci; Marker haplotype; Candidate genes ID PISUM-SATIVUM L.; QUANTITATIVE TRAIT LOCI; ROOT-ROT; DISEASE RESISTANCE; CROP PLANTS; GENETIC DIVERSITY; GERMPLASM LINES; UNITED-STATES; SNP DISCOVERY; LINKAGE MAPS AB Background: Genome-wide association (GWA) mapping has recently emerged as a valuable approach for refining the genetic basis of polygenic resistance to plant diseases, which are increasingly used in integrated strategies for durable crop protection. Aphanomyces euteiches is a soil-borne pathogen of pea and other legumes worldwide, which causes yield-damaging root rot. Linkage mapping studies reported quantitative trait loci (QTL) controlling resistance to A. euteiches in pea. However the confidence intervals (CIs) of these QTL remained large and were often linked to undesirable alleles, which limited their application in breeding. The aim of this study was to use a GWA approach to validate and refine CIs of the previously reported Aphanomyces resistance QTL, as well as identify new resistance loci. Methods: A pea-Aphanomyces collection of 175 pea lines, enriched in germplasm derived from previously studied resistant sources, was evaluated for resistance to A. euteiches in field infested nurseries in nine environments and with two strains in climatic chambers. The collection was genotyped using 13,204 SNPs from the recently developed GenoPea Infinium (R) BeadChip. Results: GWA analysis detected a total of 52 QTL of small size-intervals associated with resistance to A. euteiches, using the recently developed Multi-Locus Mixed Model. The analysis validated six of the seven previously reported main Aphanomyces resistance QTL and detected novel resistance loci. It also provided marker haplotypes at 14 consistent QTL regions associated with increased resistance and highlighted accumulation of favourable haplotypes in the most resistant lines. Previous linkages between resistance alleles and undesired late-flowering alleles for dry pea breeding were mostly confirmed, but the linkage between loci controlling resistance and coloured flowers was broken due to the high resolution of the analysis. A high proportion of the putative candidate genes underlying resistance loci encoded stress-related proteins and others suggested that the QTL are involved in diverse functions. Conclusion: This study provides valuable markers, marker haplotypes and germplasm lines to increase levels of partial resistance to A. euteiches in pea breeding. C1 [Desgroux, Aurore; L'Anthoene, Virginie; Riviere, Jean-Philippe; Vetel, Pierrick; Piriou, Christophe; Baranger, Alain; Manzanares-Dauleux, Maria; Pilet-Nayel, Marie-Laure] Inst Genet & Protect Plantes, INRA, UMR IGEPP 1349, BP 35327, F-35653 Le Rheu, France. [Desgroux, Aurore; Aubert, Gregoire; Tayeh, Nadim; Burstin, Judith; Bourion, Virginie] INRA, UMR Agroecol 1347, 17 Rue Sully, F-21065 Dijon, France. [Roux-Duparque, Martine] GSP, F-80200 Estrees Mons, France. [Riviere, Jean-Philippe; Moussart, Anne; Vetel, Pierrick; Piriou, Christophe; Baranger, Alain; Pilet-Nayel, Marie-Laure] PISOM, UMT INRA Terres Inovia, UMR IGEPP 1349, BP 35327, F-35653 Le Rheu, France. [Moussart, Anne] Terres Inovia, CS 60003, 11 Rue Monceau, F-75378 Paris, France. [Mangin, Pierre] INRA, Domaine Expt Epoisses UE0115, F-21110 Bretenieres, France. [McGee, Rebecca J.] ARS, USDA, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. [Coyne, Clarice J.] Washington State Univ, USDA, ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. [Manzanares-Dauleux, Maria] AgroCampus Ouest, UMR IGEPP 1349, 65 Rue St Brieuc, F-35042 Rennes, France. [L'Anthoene, Virginie] Nestle R&D Ctr Tours, 101 Ave Gustave Eiffel, F-37097 Tours, France. [Roux-Duparque, Martine] Chambre Agr Aisne, 1 Rue Rene Blondelle, F-02007 Laon, France. RP Pilet-Nayel, ML (reprint author), Inst Genet & Protect Plantes, INRA, UMR IGEPP 1349, BP 35327, F-35653 Le Rheu, France.; Pilet-Nayel, ML (reprint author), PISOM, UMT INRA Terres Inovia, UMR IGEPP 1349, BP 35327, F-35653 Le Rheu, France. EM Marie-Laure.Pilet@rennes.inra.fr RI UMR IGEPP, INRA/A-4054-2011 FU Aurore Desgroux, from MAP (Ministere de l'Agriculture, de l'Agroalimentaire et de la Foret, Paris, France); Terres Univia (Paris, France); INRA (Meta-programme SELGEN, France) (CropDL project); ANR project [ANR-09-GENM-026] FX This work was supported by the pre-doctoral fellowship of Aurore Desgroux, from MAP (Ministere de l'Agriculture, de l'Agroalimentaire et de la Foret, Paris, France). It was funded by Terres Univia (Paris, France) for phenotyping and SSR genotyping data and for the engineer fellowship of Virginie L'Anthoene, and by INRA (Meta-programme SELGEN, France) (CropDL project) and the ANR project (ANR-09-GENM-026) for SNP genotyping data. We greatly acknowledge GSP for having provided breeding lines improved for Aphanomyces resistance. We warmly thank the INRA experimental units of Dijon-Epoisses and Rennes-Le Rheu, as well as UNILET (Union Nationale Interprofessionnelle des Legumes Transformes), Quimperle, France, for their contribution to the field experiments. We greatly acknowledge Solene Coedel from the Genouest genotyping platform and Marie-Christine Le Paslier from the Evry genotyping platform, for their contribution to the genotyping of the pea-Aphanomyces collection. We are very grateful to Mathieu Siol and Anne-Sophie Bouchet, who provided help in genome-wide association analysis and to Gwenola LeRoy who helped in the controlled conditions experiments. We greatly thank Clement Lavaud for his critical review of the manuscript. We thank Leigh Gebbie for revising the English written style of the manuscript. NR 100 TC 2 Z9 2 U1 16 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 20 PY 2016 VL 17 AR 124 DI 10.1186/s12864-016-2429-4 PG 21 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DE2QU UT WOS:000370472600002 PM 26897486 ER PT J AU Chao, WS Dogramaci, M Horvath, DP Anderson, JV Foley, ME AF Chao, Wun S. Dogramaci, Munevver Horvath, David P. Anderson, James V. Foley, Michael E. TI Phytohormone balance and stress-related cellular responses are involved in the transition from bud to shoot growth in leafy spurge SO BMC PLANT BIOLOGY LA English DT Article DE Dormancy; Hormone profiling; Leafy spurge; RNA-seq; Vegetative growth ID UNDERGROUND ADVENTITIOUS BUDS; CONTROL PLANT-GROWTH; EUPHORBIA-ESULA L.; RNA-SEQ DATA; ABSCISIC-ACID; APICAL DOMINANCE; AUXIN TRANSPORT; DORMANCY STATUS; ABIOTIC STRESS; CROWN BUDS AB Background: Leafy spurge (Euphorbia esula L.) is an herbaceous weed that maintains a perennial growth pattern through seasonal production of abundant underground adventitious buds (UABs) on the crown and lateral roots. During the normal growing season, differentiation of bud to shoot growth is inhibited by physiological factors external to the affected structure; a phenomenon referred to as paradormancy. Initiation of shoot growth from paradormant UABs can be accomplished through removal of the aerial shoots (hereafter referred to as paradormancy release). Results: In this study, phytohormone abundance and the transcriptomes of paradormant UABs vs. shoot-induced growth at 6, 24, and 72 h after paradormancy release were compared based on hormone profiling and RNA-seq analyses. Results indicated that auxin, abscisic acid (ABA), and flavonoid signaling were involved in maintaining paradormancy in UABs of leafy spurge. However, auxin, ABA, and flavonoid levels/signals decreased by 6 h after paradormancy release, in conjunction with increase in gibberellic acid (GA), cytokinin, jasmonic acid (JA), ethylene, and brassinosteroid (BR) levels/signals. Twenty four h after paradormancy release, auxin and ABA levels/signals increased, in conjunction with increase in GA levels/signals. Major cellular changes were also identified in UABs at 24 h, since both principal component and Venn diagram analysis of transcriptomes clearly set the 24 h shoot-induced growth apart from other time groups. In addition, increase in auxin and ABA levels/signals and the down-regulation of 40 over-represented AraCyc pathways indicated that stress-derived cellular responses may be involved in the activation of stress-induced re-orientation required for initiation of shoot growth. Seventy two h after paradormancy release, auxin, cytokinin, and GA levels/signals were increased, whereas ABA, JA, and ethylene levels/signals were decreased. Conclusion: Combined results were consistent with different phytohormone signals acting in concert to direct cellular changes involved in bud differentiation and shoot growth. In addition, shifts in balance of these phytohormones at different time points and stress-related cellular responses after paradormancy release appear to be critical factors driving transition of bud to shoot growth. C1 [Chao, Wun S.; Dogramaci, Munevver; Horvath, David P.; Anderson, James V.; Foley, Michael E.] USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. RP Chao, WS (reprint author), USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM wun.chao@ars.usda.gov FU USDA-ARS CRIS project [3060-21220-029-00D] FX The authors acknowledge Wayne Sargent, USDA-ARS, Fargo, ND for his technical assistance; Cheryl Huckle, USDA-ARS, Fargo, ND for growing leafy spurge; and Dr. Mark West, USDA-ARS, Fort Collins, CO for assistance in statistical analysis. This project was supported by USDA-ARS CRIS project #3060-21220-029-00D. NR 96 TC 1 Z9 1 U1 8 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD FEB 20 PY 2016 VL 16 AR 47 DI 10.1186/s12870-016-0735-2 PG 21 WC Plant Sciences SC Plant Sciences GA DE1TC UT WOS:000370408300001 PM 26897527 ER PT J AU Bodnar, AL Schroder, MN Scott, MP AF Bodnar, Anastasia L. Schroder, Megan N. Scott, M. Paul TI Recurrent Selection for Transgene Activity Levels in Maize Results in Proxy Selection for a Native Gene with the Same Promoter SO PLOS ONE LA English DT Article ID GREEN FLUORESCENT PROTEIN; ZEIN; EXPRESSION; ENDOSPERM; MARKER; PLANTS; SEED AB High activity levels of a transgene can be very useful, making a transgene easier to evaluate for safety and efficacy. High activity levels can also increase the economic benefit of the production of high value proteins in transgenic plants. The goal of this research is to determine if recurrent selection for activity of a transgene will result in higher activity, and if selection for activity of a transgene controlled by a native promoter will also increase protein levels of the native gene with the same promoter. To accomplish this goal we used transgenic maize containing a construct encoding green fluorescent protein controlled by the promoter for the maize endosperm-specific 27kDa gamma zein seed storage protein. We carried out recurrent selection for fluorescence intensity in two breeding populations. After three generations of selection, both selected populations were significantly more fluorescent and had significantly higher levels of 27kDa gamma zein than the unselected control populations. These higher levels of the 27kDa gamma zein occurred independently of the presence of the transgene. The results show that recurrent selection can be used to increase activity of a transgene and that selection for a transgene controlled by a native promoter can increase protein levels of the native gene with the same promoter via proxy selection. Moreover, the increase in native gene protein level is maintained in the absence of the transgene, demonstrating that proxy selection can be used to produce non-transgenic plants with desired changes in gene expression. C1 [Bodnar, Anastasia L.; Schroder, Megan N.] Iowa State Univ, Interdept Genet Program, Ames, IA USA. [Scott, M. Paul] ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Bodnar, Anastasia L.] Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Riverdale, MD USA. [Schroder, Megan N.] DuPont Pioneer, Johnston, IA USA. RP Scott, MP (reprint author), ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA USA. EM paul.scott@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service [3625-21000-055D]; United States Department of Agriculture Cooperative State Ressearch, Education and Extension Service FX This work was funded by the United States Department of Agriculture Agricultural Research Service (http://www.ars.usda.gov/main/main.htm) CRIS project number 3625-21000-055D, MPS and the United States Department of Agriculture Cooperative State Ressearch, Education and Extension Service (no URL available) special appropriation Biotechnology Test Production, IA: Recovery and Purification of Recombinant Proteins from Plants for Therapeutics and Industrial Enzymes, ALB, MNS. NR 22 TC 0 Z9 0 U1 2 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 19 PY 2016 VL 11 IS 2 AR e0148587 DI 10.1371/journal.pone.0148587 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3DG UT WOS:000371223400019 PM 26895451 ER PT J AU Ali, SS Nugent, B Mullins, E Doohan, FM AF Ali, Shahin S. Nugent, Brian Mullins, Ewen Doohan, Fiona M. TI Fungal-mediated consolidated bioprocessing: the potential of Fusarium oxysporum for the lignocellulosic ethanol industry SO AMB EXPRESS LA English DT Review DE Bioethanol; Fungi; Fusarium; Consolidated bioprocessing; Lignocellulose ID SACCHAROMYCES-CEREVISIAE STRAIN; ANAEROBIC XYLOSE FERMENTATION; BIOETHANOL PRODUCTION; SIMULTANEOUS SACCHARIFICATION; CELLULOSIC ETHANOL; TRICHODERMA-REESEI; NEUROSPORA-CRASSA; WHEAT-STRAW; GENETIC MANIPULATION; CELLULOLYTIC ENZYMES AB Microbial bioprocessing of lignocellulose to bioethanol still poses challenges in terms of substrate catabolism. The most important challenge is to overcome substrate recalcitrance and to thus reduce the number of steps needed to biorefine lignocellulose. Conventionally, conversion involves chemical pretreatment of lignocellulose, followed by hydrolysis of biomass to monomer sugars that are subsequently fermented into bioethanol. Consolidated bioprocessing (CBP) has been suggested as an efficient and economical method of manufacturing bioethanol from lignocellulose. CBP integrates the hydrolysis and fermentation steps into a single process, thereby significantly reducing the amount of steps in the biorefining process. Filamentous fungi are remarkable organisms that are naturally specialised in deconstructing plant biomass and thus they have tremendous potential as components of CBP. The fungus Fusarium oxysporum has potential for CBP of lignocellulose to bioethanol. Here we discuss the complexity and potential of CBP, the bottlenecks in the process, and the potential influence of fungal genetic diversity, substrate complexity and new technologies on the efficacy of CPB of lignocellulose, with a focus on F. oxysporum. C1 [Ali, Shahin S.; Nugent, Brian; Doohan, Fiona M.] Univ Coll Dublin, Mol Plant Microbe Interact Lab, Sch Biol & Environm Sci, Dublin 4, Ireland. [Ali, Shahin S.] USDA ARS, SPCL, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Mullins, Ewen] Teagasc Res Ctr, Dept Crop Sci, Oak Pk, Carlow, Ireland. RP Doohan, FM (reprint author), Univ Coll Dublin, Mol Plant Microbe Interact Lab, Sch Biol & Environm Sci, Dublin 4, Ireland. EM fiona.doohan@ucd.ie OI Mullins, Ewen/0000-0003-3005-4264 FU Irish Department of Agriculture, Fisheries and Food Research Stimulus Fund [RSF 07 513]; Earth and Natural Sciences (ENS) Doctoral Studies Programme; Higher Education Authority (HEA) through the Programme for Research at Third Level Education, Cycle 5 (PRTLI-5); European Regional Development Fund (ERDF) FX Shahin Ali was supported by the Irish Department of Agriculture, Fisheries and Food Research Stimulus Fund (RSF 07 513) and Brian Nugent was supported by the Earth and Natural Sciences (ENS) Doctoral Studies Programme. The ENS programme is funded by the Higher Education Authority (HEA) through the Programme for Research at Third Level Education, Cycle 5 (PRTLI-5) and is co-funded by the European Regional Development Fund (ERDF). The authors thank Bredagh Moran and Brian Fagan for technical assistance. NR 136 TC 0 Z9 1 U1 6 U2 45 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2191-0855 J9 AMB EXPRESS JI AMB Express PD FEB 18 PY 2016 VL 6 AR 13 DI 10.1186/s13568-016-0185-0 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DE1QD UT WOS:000370400600001 PM 26888202 ER PT J AU Barreiro-Fernandez, L Bujan, S Miranda, D Dieguez-Aranda, U Gonzalez-Ferreiro, E AF Barreiro-Fernandez, Laura Bujan, Sandra Miranda, David Dieguez-Aranda, Ulises Gonzalez-Ferreiro, Eduardo TI Accuracy assessment of LiDAR-derived digital elevation models in a rural landscape with complex terrain SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE airborne laser scanning; digital elevation model; accuracy analysis; FUSION software ID AIRBORNE LIDAR; DEM ACCURACY; INTERPOLATION; GENERATION; SLOPE AB Digital elevation models (DEMs) are essential in many professional areas as they produce georeferenced elevation data that are critical for a wide range of studies, computations, decision-making processes, and derived products. Quality control thus becomes necessary to quantify the accuracy of the information provided. We assessed the accuracy of elevation data estimated by DEMs derived from LiDAR data representing diverse land cover types. For this purpose, we used the FUSION software and explored variations in accuracy in relation to the following factors: input data, interpolation methods, terrain slope, heterogeneity of land cover, and LiDAR point density. We selected and measured 1157 checkpoints by using total station and GPS techniques and following a stratified random design in order to validate the LiDAR-derived DEMs. We used robust estimators, nonparametric tests, and analysis of variance to examine the elevation errors. The study findings showed the following: (1) using the full set of LiDAR returns did not improve elevation accuracy relative to using the last-return data set; (2) using the minimum switch for interpolation did not improve accuracy compared to the default behavior of the interpolator; (3) land cover and slope significantly affected accuracy; (4) DEMs tended to underestimate elevation; and (5) the mean density of the returns classified as ground was significantly affected by land cover and slope factors. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) C1 [Barreiro-Fernandez, Laura; Bujan, Sandra; Miranda, David] Univ Santiago de Compostela, Dept Agroforestry Engn, Land Lab, Escuela Politecn Super, R Benigno Ledo S-N, Lugo 27002, Spain. [Dieguez-Aranda, Ulises; Gonzalez-Ferreiro, Eduardo] Univ Santiago de Compostela, Dept Agroforestry Engn, Sustainable Forest Management Unit UXFS, Escuela Politecn Super, R Benigno Ledo S-N, Lugo 27002, Spain. [Gonzalez-Ferreiro, Eduardo] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Gonzalez-Ferreiro, Eduardo] US Forest Serv, USDA, Pacific NW Res Stn, LARSE, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Gonzalez-Ferreiro, E (reprint author), Univ Santiago de Compostela, Dept Agroforestry Engn, Sustainable Forest Management Unit UXFS, Escuela Politecn Super, R Benigno Ledo S-N, Lugo 27002, Spain.; Gonzalez-Ferreiro, E (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA.; Gonzalez-Ferreiro, E (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, LARSE, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM edu.g.ferreiro@gmail.com RI Dieguez-Aranda, Ulises/E-4302-2012; Gonzalez-Ferreiro, Eduardo/Q-9709-2016 OI Dieguez-Aranda, Ulises/0000-0002-4640-6714; Gonzalez-Ferreiro, Eduardo/0000-0002-4565-2155 NR 52 TC 0 Z9 0 U1 8 U2 24 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD FEB 18 PY 2016 VL 10 AR 016014 DI 10.1117/1.JRS.10.016014 PG 17 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DE6WY UT WOS:000370776600001 ER PT J AU Hampel, D Shahab-Ferdows, S Adair, LS Bentley, ME Flax, VL Jamieson, DJ Ellington, SR Tegha, G Chasela, CS Kamwendo, D Allen, LH AF Hampel, Daniela Shahab-Ferdows, Setareh Adair, Linda S. Bentley, Margaret E. Flax, Valerie L. Jamieson, Denise J. Ellington, Sascha R. Tegha, Gerald Chasela, Charles S. Kamwendo, Debbie Allen, Lindsay H. TI Thiamin and Riboflavin in Human Milk: Effects of Lipid-Based Nutrient Supplementation and Stage of Lactation on Vitamer Secretion and Contributions to Total Vitamin Content SO PLOS ONE LA English DT Article ID CANCER RESISTANCE PROTEIN; BREAST-MILK; RANDOMIZED-TRIAL; MATERNAL STATUS; B VITAMINS; WOMEN; TRANSPORTER; TRANSMISSION; POSTPARTUM; DEFICIENCY AB While thiamin and riboflavin in breast milk have been analyzed for over 50 years, less attention has been given to the different forms of each vitamin. Thiamin-monophosphate (TMP) and free thiamin contribute to total thiamin content; flavin adenine-dinucleotide (FAD) and free riboflavin are the main contributors to total riboflavin. We analyzed milk collected at 2 (n = 258) or 6 (n = 104), and 24 weeks (n = 362) from HIV-infected Malawian mothers within the Breastfeeding, Antiretrovirals and Nutrition (BAN) study, randomly assigned at delivery to lipid-based nutrient supplements (LNS) or a control group, to investigate each vitamer's contribution to total milk vitamin content and the effects of supplementation on the different thiamin and riboflavin vitamers at early and later stages of lactation, and obtain insight into the transport and distribution of these vitamers in human milk. Thiamin vitamers were derivatized into thiochrome-esters and analyzed by high-performance liquid-chromatography-fluorescence-detection (HPLC-FLD). Riboflavin and FAD were analyzed by ultra-performance liquid-chromatography-tandem-mass-spectrometry (ULPC-MS/MS). Thiamin-pyrophosphate (TPP), identified here for the first time in breast milk, contributed 1.9-4.5% to total thiamin. Free thiamin increased significantly from 2/6 to 24 weeks regardless of treatment indicating an active transport of this vitamer in milk. LNS significantly increased TMP and free thiamin only at 2 weeks compared to the control: median 170 versus 151 mu g/L (TMP), 13.3 versus 10.5 mu g/L (free thiamin, p<0.05 for both, suggesting an up-regulated active mechanism for TMP and free thiamin accumulation at early stages of lactation. Free riboflavin was consistently and significantly increased with LNS (range: 14.8-19.6 mu g/L (LNS) versus 5.0-7.4 mu g/L (control), p<0.001), shifting FAD: riboflavin relative amounts from 92-94:6-8% to 85:15%, indicating a preferred secretion of the free form into breast milk. The continuous presence of FAD in breast milk suggests an active transport and secretion system for this vitamer or possibly formation of this co-enymatic form in the mammary gland. C1 [Hampel, Daniela; Shahab-Ferdows, Setareh; Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Hampel, Daniela; Allen, Lindsay H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Adair, Linda S.; Bentley, Margaret E.; Flax, Valerie L.] Univ N Carolina, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Jamieson, Denise J.; Ellington, Sascha R.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Tegha, Gerald; Chasela, Charles S.; Kamwendo, Debbie] UNC Project, Lilongwe, Malawi. [Chasela, Charles S.] Univ Witwatersrand, Sch Publ Hlth, Div Epidemiol & Biostat, Johannesburg, South Africa. RP Hampel, D (reprint author), ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA.; Hampel, D (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. EM dhampel@ucdavis.edu FU Prevention Research Centers Special Interest Project of the Centers for Disease Control and Prevention [SIP 13-01 U48-CCU409660-09, SIP 26-04 U48-DP000059-01, SIP 22-09 U48-DP001944-01]; Bill & Melinda Gates Foundation [OPP53107, OPP1061055]; National Institute of Allergy and Infectious Diseases; University of North Carolina Center for AIDS Research [P30-AI50410]; Carolina Population Center [R24 HD050924]; NIH Fogarty AIDS International Training and Research Program [DHHS/NIH/FIC 2-D43 Tw01039-06, R24 Tw00798]; NIH Fogarty AIDS International Training and Research Program (American Recovery and Reinvestment Act); intramural USDA-ARS Project [5306-51000-003-00D]; Elizabeth Glaser Pediatric AIDS Foundation; United Nations Children's Fund; World Food Program; Malawi Ministry of Health and Population; Johnson Johnson; U.S. Agency for International Development FX The Breastfeeding, Antiretrovirals, and Nutrition Study was supported by grants from the Prevention Research Centers Special Interest Project of the Centers for Disease Control and Prevention (SIP 13-01 U48-CCU409660-09, SIP 26-04 U48-DP000059-01, and SIP 22-09 U48-DP001944-01), Bill & Melinda Gates Foundation (OPP53107 and OPP1061055), the National Institute of Allergy and Infectious Diseases, the University of North Carolina Center for AIDS Research (P30-AI50410), the Carolina Population Center (R24 HD050924), the NIH Fogarty AIDS International Training and Research Program (DHHS/NIH/FIC 2-D43 Tw01039-06 and R24 Tw00798; the American Recovery and Reinvestment Act), and intramural USDA-ARS Project (5306-51000-003-00D). The antiretrovirals used in the BAN study were donated by Abbott Laboratories, GlaxoSmithKline, Boehringer Ingelheim, Roche Pharmaceuticals, and Bristol-Myers Squibb. The Call to Action PMTCT program was supported by the Elizabeth Glaser Pediatric AIDS Foundation, the United Nations Children's Fund, the World Food Program, the Malawi Ministry of Health and Population, Johnson & Johnson, and the U.S. Agency for International Development. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 2 Z9 2 U1 3 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 17 PY 2016 VL 11 IS 2 AR e0149479 DI 10.1371/journal.pone.0149479 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3BJ UT WOS:000371218400089 PM 26886782 ER PT J AU Sainju, UM AF Sainju, Upendra M. TI Can Novel Management Practice Improve Soil and Environmental Quality and Sustain Crop Yield Simultaneously? SO PLOS ONE LA English DT Article ID NORTHERN GREAT-PLAINS; LONG-TERM TILLAGE; NITROUS-OXIDE; CARBON-DIOXIDE; GREENHOUSE GASES; NO-TILL; SYSTEMS; AGROECOSYSTEMS; IRRIGATION; INTENSITY AB Little is known about management practices that can simultaneously improve soil and environmental quality and sustain crop yields. The effects of novel and traditional management practices that included a combination of tillage, crop rotation, and N fertilization on soil C and N, global warming potential (GWP), greenhouse gas intensity (GHGI), and malt barley (Hordeum vulgarie L.) yield and quality were examined under non-irrigated and irrigated cropping systems from 2008 to 2011 in eastern Montana and western North Dakota, USA. In loamy soil under non-irrigated condition in eastern Montana, novel and traditional management practices were no-till malt barley-pea (Pisum sativum L.) with 80 kg N ha(-1) and conventional till malt barley-fallow with 80 kg N ha(-1), respectively. In sandy loam soil under irrigated and non-irrigated conditions in western North Dakota, novel and traditional management practices included no-till malt barley-pea with 67 (non-irrigated) to 134 kg N ha(-1) (irrigated) and conventional till malt barley with 67 (non-irrigated) to 134 kg N ha(-1) (irrigated), respectively. Compared with the traditional management practice, soil organic C (SOC) and total N (STN) at 0-120 cm were 5% greater with the novel management practice under non-irrigated condition in eastern Montana and under irrigated condition in western North Dakota, but were not different under non-irrigated condition in western North Dakota. In both places under irrigated and non-irrigated conditions, total applied N rate, residual soil NO3-N content at 0-120 cm, global warming potential (GWP), and greenhouse gas intensity (GHGI) were 15 to 70% lower with the novel than the traditional management practice. Malt barley yield and quality were not different between the two practices in both places. Novel management practices, such as no-till malt barley-pea with reduced N rate, can simultaneously enhance soil and environmental quality, reduce N input, and sustain crop yield compared with traditional practices in the northern Great Plains, USA. C1 [Sainju, Upendra M.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Sainju, UM (reprint author), ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov FU USDA-ARS-GRACEnet project; USDA-ARS, Sidney, MT FX Funded in part by USDA-ARS-GRACEnet project. Other funding was contributed by USDA-ARS, Sidney, MT. No proposal was submitted and therefore no grant number. NR 42 TC 0 Z9 0 U1 2 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 17 PY 2016 VL 11 IS 2 AR e0149005 DI 10.1371/journal.pone.0149005 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3BJ UT WOS:000371218400058 PM 26886932 ER PT J AU Yang, SS Fresnedo-Ramirez, J Wang, MH Cote, L Schweitzer, P Barba, P Takacs, EM Clark, M Luby, J Manns, DC Sacks, G Mansfield, AK Londo, J Fennell, A Gadoury, D Reisch, B Cadle-Davidson, L Sun, Q AF Yang, Shanshan Fresnedo-Ramirez, Jonathan Wang, Minghui Cote, Linda Schweitzer, Peter Barba, Paola Takacs, Elizabeth M. Clark, Matthew Luby, James Manns, David C. Sacks, Gavin Mansfield, Anna Katharine Londo, Jason Fennell, Anne Gadoury, David Reisch, Bruce Cadle-Davidson, Lance Sun, Qi TI A next-generation marker genotyping platform (AmpSeq) in heterozygous crops: a case study for marker-assisted selection in grapevine SO HORTICULTURE RESEARCH LA English DT Article ID VITIS-VINIFERA L.; POWDERY MILDEW RESISTANCE; MOLECULAR MARKERS; MICROSATELLITE MARKERS; CANDIDATE GENES; COMPLEX TRAITS; SEQUENCING GBS; LINKAGE MAPS; GENUS VITIS; FRUIT CROPS AB Marker-assisted selection (MAS) is often employed in crop breeding programs to accelerate and enhance cultivar development, via selection during the juvenile phase and parental selection prior to crossing. Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery. To bridge the gap between marker development and MAS implementation, this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery, low-cost genotyping through amplicon sequencing (AmpSeq) and decision making. The results document the development of a MAS package derived from genotyping-by-sequencing using three traits (flower sex, disease resistance and acylated anthocyanins) in grapevine breeding. The vast majority of sequence reads (>= 99%) were from the targeted regions. Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data, most amplicons (83 to 87%) had <10% missing data, and read depth had a median of 220-244 x. Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy, flexibility, speed, high-throughput, low-cost and easily automated analysis. C1 [Yang, Shanshan; Takacs, Elizabeth M.; Reisch, Bruce] Cornell Univ, Sch Integrat Plant Sci, Hort Sect, Geneva, NY 14456 USA. [Fresnedo-Ramirez, Jonathan; Wang, Minghui; Sun, Qi] Cornell Univ, Bioinformat Facil, Ithaca, NY 14853 USA. [Cote, Linda; Schweitzer, Peter] Cornell Univ, Inst Biotechnol, Ithaca, NY 14853 USA. [Barba, Paola] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Clark, Matthew; Luby, James] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Manns, David C.; Mansfield, Anna Katharine] Cornell Univ, Dept Food Sci, Geneva, NY 14456 USA. [Sacks, Gavin] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Londo, Jason; Cadle-Davidson, Lance] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. [Fennell, Anne] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Gadoury, David] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. RP Sun, Q (reprint author), Cornell Univ, Bioinformat Facil, Ithaca, NY 14853 USA.; Cadle-Davidson, L (reprint author), USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. EM lance.cadledavidson@ars.usda.gov; qisun@cornell.edu OI Sacks, Gavin/0000-0002-1403-0505; Fresnedo Ramirez, Jonathan/0000-0003-1985-2513; Clark, Matthew/0000-0002-1771-1955 NR 90 TC 3 Z9 3 U1 7 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD FEB 17 PY 2016 VL 3 AR 16002 DI 10.1038/hortres.2016.2 PG 12 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA DE8GI UT WOS:000370873400001 PM 27257505 ER PT J AU Castro, EB Kane, EC Feres, RJF Ochoa, R Bauchan, GR AF Castro, Elizeu B. Kane, Ethan C. Feres, Reinaldo J. F. Ochoa, Ronald Bauchan, Gary R. TI Definition of Tenuipalpus sensu stricto (Acari, Tenuipalpidae), with redescription of Tenuipalpus caudatus (Duges) and description of a new species from Costa Rica SO INTERNATIONAL JOURNAL OF ACAROLOGY LA English DT Article DE Flat mites; systematics; taxonomy; crests; Tenuipalpus palmatus Donnadieu; low-temperature scanning electron microscopy; remote-sampling; body projections ID AUSTRALIAN SEDGES CYPERACEAE; MITES ACARI; TETRANYCHOIDEA; TROMBIDIFORMES; GENUS; FAUNA AB The taxonomic history of the genus Tenuipalpus Donnadieu is discussed and Tenuipalpus caudatus (Duges) (=Tenuipalpus palmatus Donnadieu) is redescribed based on specimens from Portugal intercepted at ports of entry in the United States, and references including photographic records of the neotype of T. caudatus. In addition, a proposed new species, Tenuipalpus erbei sp. nov. is described from Costa Rica. Our results show that T. caudatus, T. erbei sp. nov. and another 36 known species of Tenuipalpus share a pair of lateral body projections associated with setae c3, considered a synapomorphy for the newly defined group, Tenuipalpus sensu stricto. We also show that its members share other character states, although these features are found elsewhere in Tenuipalpus and also in Ultratenuipalpus, indicating their origins are within Tenuipalpus. A list of Tenuipalpus sensu stricto species is presented. C1 [Castro, Elizeu B.] Univ Estadual Paulista, UNESP, Programa Pos Grad Biol Anim, Sao Jose Do Rio Preto, SP, Brazil. [Kane, Ethan C.] USDA, PPQ, APHIS, Riverdale, MD USA. [Feres, Reinaldo J. F.] UNESP, Dept Zool & Bot, Sao Jose Do Rio Preto, SP, Brazil. [Ochoa, Ronald] USDA ARS, SEL, BARC, Beltsville, MD USA. [Bauchan, Gary R.] USDA ARS, Beltsville Agr Res Ctr, ECMU, Beltsville, MD 20705 USA. RP Castro, EB (reprint author), Univ Estadual Paulista, UNESP, Programa Pos Grad Biol Anim, Sao Jose Do Rio Preto, SP, Brazil. EM elizeu_unesp@yahoo.com.br RI Castro, Elizeu/A-9915-2015 OI Castro, Elizeu/0000-0002-8056-6893 FU Smithsonian Natural History Museum; ALAS Project (Finca La Selva, Costa Rica); University of Maryland (Dept. of Entomology); National Agricultural Library (NAL-USDA); United States Department of Agriculture (USDA) National Program; APHIS; "Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior" (CAPES) [BEX 7768/13-3]; "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq) [303435/2013-5] FX We thank the Smithsonian Natural History Museum, ALAS Project (Finca La Selva, Costa Rica), University of Maryland (Dept. of Entomology), National Agricultural Library (NAL-USDA), United States Department of Agriculture (USDA) National Program and APHIS for their support and assistance with references, permits and funding for this study. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer. This study was supported by the "Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior" (CAPES) (Proc. no BEX 7768/13-3) and "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq) (Proc. no 303435/2013-5), by fellowship and research grant to first and third authors, respectively. NR 52 TC 3 Z9 3 U1 2 U2 3 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0164-7954 EI 1945-3892 J9 INT J ACAROL JI Int. J. Acarol. PD FEB 17 PY 2016 VL 42 IS 2 BP 106 EP 126 DI 10.1080/01647954.2015.1130941 PG 21 WC Entomology SC Entomology GA DE9UC UT WOS:000370983900005 ER PT J AU Johnson, KL Williams, JG Maleki, SJ Hurlburt, BK London, RE Mueller, GA AF Johnson, Katina L. Williams, Jason G. Maleki, Soheila J. Hurlburt, Barry K. London, Robert E. Mueller, Geoffrey A. TI Enhanced Approaches for Identifying Amadori Products: Application to Peanut Allergens SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE peanuts (Arachis hypogaea); allergen; advanced glycation end products; Amadori products; Maillard reactions; Shannon entropy ID GLYCATION END-PRODUCTS; TANDEM MASS-SPECTROMETRY; PROCESSED PEANUTS; MAILLARD REACTION; PEPTIDES; PROTEINS; BINDING; FOODS AB The dry roasting of peanuts is suggested to influence allergic sensitization as a result of the formation of advanced glycation end products (AGEs) on peanut proteins. Identifying AGEs is technically challenging. The AGEs of a peanut allergen were probed with nano-scale liquid chromatography electrospray ionization mass spectrometry (nanoLC-ESI-MS) and tandem mass spectrometry (MS/MS) analyses. Amadori product ions matched to expected peptides and yielded fragments that included a loss of three waters and HCHO. As a result of the paucity of b and y ions in the MS/MS spectrum, standard search algorithms do not perform well. Reactions with isotopically labeled sugars confirmed that the peptides contained Amadori products. An algorithm was developed on the basis of information content (Shannon entropy) and the loss of water and HCHO. Results with test data show that the algorithm finds the correct spectra with high precision, reducing the time needed to manually inspect data. Computational and technical improvements allowed for better identification of the chemical differences between modified and unmodified proteins. C1 [Johnson, Katina L.; Williams, Jason G.] NIEHS, Epigenet & Stem Cell Biol Lab, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [London, Robert E.; Mueller, Geoffrey A.] NIEHS, Genome Integr & Struct Biol Lab, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. [Maleki, Soheila J.; Hurlburt, Barry K.] ARS, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Mueller, GA (reprint author), NIEHS, Genome Integr & Struct Biol Lab, 111 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM geoffrey.mueller@nih.gov FU National Institute of Environmental Health Sciences, National Institutes of Health [Z01-ES102885-01, Z01-ES102488-05] FX This research was supported in part by Research Projects Z01-ES102885-01 to Robert E. London and Z01-ES102488-05 to Jason G. Williams in the Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health. NR 26 TC 0 Z9 0 U1 9 U2 25 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 17 PY 2016 VL 64 IS 6 BP 1406 EP 1413 DI 10.1021/acs.jafc.5b05492 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DE4FF UT WOS:000370583900023 PM 26811263 ER PT J AU Natarajan, S Khan, F Song, QJ Lakshman, S Cregan, P Scott, R Shipe, E Garrett, W AF Natarajan, Savithiry Khan, Farooq Song, Qijian Lakshman, Sukla Cregan, Perry Scott, Roy Shipe, Emerson Garrett, Wesley TI Characterization of Soybean Storage and Allergen Proteins Affected by Environmental and Genetic Factors SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soybean; G. sofa; G. max; 2D-PAGE; MALDI-TOF-MS; beta-conglycinin; glycinin; allergen; location; genotype; environment ID M BD 30K; PROTEOMIC ANALYSIS; GERMPLASM COLLECTIONS; SEED COMPOSITION; CULTIVARS; GLYCININ; GENOTYPE; IDENTIFICATION; VARIABILITY; WILD AB There is limited information on the influence of genetic and environmental variability on soybean protein composition. This study aimed to determine the role of genotype (G), environments (E), and the interrelationship of genotype and environment (GxE) on soybean seed protein. Three sets of nine soybean genotypes were grown in replicated trials at Maryland, South Carolina, and South Dakota. At each location, the nine genotypes were grown with two planting/sowing dates. We applied two-dimensional gel electrophoresis and mass spectrometry to study the variability of soybean storage and allergen proteins. Statistical analysis of 47 storage and 8 allergen proteins, in terms of differentially expressed protein spots significant at the p<0.005 level, was performed. We found more spots that showed statistically significant differences in expression among E compared to G and GXE interaction. C1 [Natarajan, Savithiry; Song, Qijian; Cregan, Perry] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Khan, Farooq] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Lakshman, Sukla] USDA ARS, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Scott, Roy] USDA ARS, Crop Prod & Protect Oilseeds & Biosci, Beltsville, MD 20705 USA. [Shipe, Emerson] Clemson Univ, Dept Entomol Soils & Plant Sci, Clemson, SC 29634 USA. [Garrett, Wesley] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. RP Natarajan, S (reprint author), USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. EM savi.natarajan@ars.usda.gov FU ARS project [1245-21220-232-00D] FX Funding for this research was provided by ARS project 1245-21220-232-00D. NR 52 TC 2 Z9 2 U1 4 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 17 PY 2016 VL 64 IS 6 BP 1433 EP 1445 DI 10.1021/acs.jafc.5b05172 PG 13 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DE4FF UT WOS:000370583900026 PM 26807503 ER PT J AU Kim, J Yang, JY Yang, RH Sicher, RC Chang, C Tucker, ML AF Kim, Joonyup Yang, Jinyoung Yang, Ronghui Sicher, Richard C. Chang, Caren Tucker, Mark L. TI Transcriptome Analysis of Soybean Leaf Abscission Identifies Transcriptional Regulators of Organ Polarity and Cell Fate SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE transcription factors; network; abscission; soybean; Glycine max; organ polarity; cell fate ID RECEPTOR-LIKE KINASE; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; ZONE DEVELOPMENT; YABBY ACTIVITIES; RNA-SEQ; AUXIN; MERISTEM; TOMATO; SEPARATION AB Abscission, organ separation, is a developmental process that is modulated by endogenous and environmental factors. To better understand the molecular events underlying the progression of abscission in soybean, an agriculturally important legume, we performed RNA sequencing (RNA-seq) of RNA isolated from the leaf abscission zones (LAZ) and petioles (Non-AZ, NAZ) after treating stem/petiole explants with ethylene for 0, 12, 24, 48, and 72 h. As expected, expression of several families of cell wall modifying enzymes and many pathogenesis related (PR) genes specifically increased in the LAZ as abscission progressed. Here, we focus on the 5,206 soybean genes we identified as encoding transcription factors (TFs). Of the 5,206 TFs, 1,088 were differentially up or down-regulated more than eight-fold in the LAZ over time, and, within this group, 188 of the TFs were differentially regulated more than eight-fold in the LAZ relative to the NAZ. These 188 abscission-specific TFs include several TFs containing domains for homeobox, MYB, Zinc finger, bHLH, AP2, NAG, WRKY, YABBY, and auxin-related motifs. To discover the connectivity among the TFs and highlight developmental processes that support organ separation, the 188 abscission-specific TFs were then clustered based on a >four-fold up- or down-regulation in two consecutive time points (i.e., 0 and 12 h, 12 and 24 h, 24 and 48 h, or 48 and 72 h). By requiring a sustained change in expression over two consecutive time intervals and not just one or several time intervals, we could better tie changes in TFs to a particular process or phase of abscission. The greatest number of TFs clustered into the 0 and 12 h group. Transcriptional network analysis for these abscission-specific TFs indicated that most of these TFs are known as key determinants in the maintenance of organ polarity, lateral organ growth, and cell fate. The abscission specific expression of these TFs prior to the onset of abscission and their functional properties as defined by studies in Arabidopsis indicate that these TFs are involved in defining the separation cells and initiation of separation within the AZ by balancing organ polarity, roles of plant hormones, and cell differentiation. C1 [Kim, Joonyup; Yang, Ronghui; Tucker, Mark L.] USDA, Soybean Genom & improvement Lab, ARS, Beltsville, MD 20705 USA. [Kim, Joonyup; Chang, Caren] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. [Yang, Jinyoung; Sicher, Richard C.] USDA, Crop Syst & Global Change Lab, ARS, Beltsville, MD 20705 USA. RP Kim, J; Tucker, ML (reprint author), USDA, Soybean Genom & improvement Lab, ARS, Beltsville, MD 20705 USA.; Kim, J (reprint author), Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. EM joonyup.kim@ars.usda.gov; mark.tucker@ars.usda.gov FU Binational Agricultural and Development Fund (BARD) [US-4571-12C] FX This work was supported by a Binational Agricultural and Development Fund (BARD) US-4571-12C grant to MLT. NR 87 TC 2 Z9 2 U1 5 U2 26 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 17 PY 2016 VL 7 AR 125 DI 10.3389/fpls.2016.00125 PG 16 WC Plant Sciences SC Plant Sciences GA DD9IC UT WOS:000370237900001 PM 26925069 ER PT J AU Gruber, A Su, CH Crow, WT Zwieback, S Dorigo, WA Wagner, A AF Gruber, A. Su, C. -H. Crow, W. T. Zwieback, S. Dorigo, W. A. Wagner, W. TI Estimating error cross-correlations in soil moisture data sets using extended collocation analysis SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE soil moisture; triple collocation; error characterization; error cross-correlation; data assimilation; validation ID TRIPLE COLLOCATION; MODEL SIMULATIONS; SMOS VALIDATION; NEAR-SURFACE; SATELLITE; RETRIEVALS; PRODUCTS; NETWORK; EUROPE; ASCAT AB Global soil moisture records are essential for studying the role of hydrologic processes within the larger earth system. Various studies have shown the benefit of assimilating satellite-based soil moisture data into water balance models or merging multisource soil moisture retrievals into a unified data set. However, this requires an appropriate parameterization of the error structures of the underlying data sets. While triple collocation (TC) analysis has been widely recognized as a powerful tool for estimating random error variances of coarse-resolution soil moisture data sets, the estimation of error cross covariances remains an unresolved challenge. Here we propose a methodreferred to as extended collocation (EC) analysisfor estimating error cross-correlations by generalizing the TC method to an arbitrary number of data sets and relaxing the therein made assumption of zero error cross-correlation for certain data set combinations. A synthetic experiment shows that EC analysis is able to reliably recover true error cross-correlation levels. Applied to real soil moisture retrievals from Advanced Microwave Scanning Radiometer-EOS (AMSR-E) C-band and X-band observations together with advanced scatterometer (ASCAT) retrievals, modeled data from Global Land Data Assimilation System (GLDAS)-Noah and in situ measurements drawn from the International Soil Moisture Network, EC yields reasonable and strong nonzero error cross-correlations between the two AMSR-E products. Against expectation, nonzero error cross-correlations are also found between ASCAT and AMSR-E. We conclude that the proposed EC method represents an important step toward a fully parameterized error covariance matrix for coarse-resolution soil moisture data sets, which is vital for any rigorous data assimilation framework or data merging scheme. C1 [Gruber, A.; Dorigo, W. A.; Wagner, W.] Vienna Univ Technol, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria. [Gruber, A.; Su, C. -H.] Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic 3052, Australia. [Crow, W. T.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Zwieback, S.] ETH, Inst Environm Engn, Zurich, Switzerland. [Dorigo, W. A.] Univ Ghent, Lab Hydrol & Water Management, B-9000 Ghent, Belgium. RP Gruber, A (reprint author), Vienna Univ Technol, Dept Geodesy & Geoinformat, A-1040 Vienna, Austria.; Gruber, A (reprint author), Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic 3052, Australia. EM alexander.gruber@geo.tuwien.ac.at OI Wagner, Wolfgang/0000-0001-7704-6857 FU European Union [603608]; University of Melbourne FX We thank Robert Parinussa and Alexandra Konings for valuable discussions. The used AMSR-E and GLDAS-Noah data are available from the NASA Goddard Space Flight Center (ftp://hydro1.sci.gsfc.nasa.gov/data/s4pa_TS2/WAOB/; ftp://hydro1.sci.gsfc.nasa.gov/data/s4pa_TS2/GLDAS_V1/). ASCAT data are available from EUMETSAT's H-SAF data portal (http://hsaf.meteoam.it/soil-moisture.php). In situ data used in this study are provided by the AMMA-CATCH, ARM, COSMOS, GTK, HOBE, ICN, MAQU, MOL-RAO, OZNET, PBO-H2O, REMEDHUS, SASMAS, SCAN, SMOS-MANIA, SNOTEL, SWEX-POLAND, UDC-SMOS, UMBRIA, USCRN, and USDA-ARS networks and are available from the ISMN data portal (http://ismn.geo.tuwien.ac.at/data-access/). This study was carried out within the eartH2Observe project (European Union's Seventh Framework Programme, grant 603608) and with support under The University of Melbourne's Early Career Researcher Grant Scheme. NR 53 TC 4 Z9 4 U1 6 U2 10 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD FEB 16 PY 2016 VL 121 IS 3 BP 1208 EP 1219 DI 10.1002/2015JD024027 PG 12 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DF6QW UT WOS:000371481700011 ER PT J AU Johnson, RC Vance-Borland, K AF Johnson, R. C. Vance-Borland, Ken TI Linking Genetic Variation in Adaptive Plant Traits to Climate in Tetraploid and Octoploid Basin Wildrye [Leymus cinereus (Scribn. & Merr.) A. Love] in the Western US SO PLOS ONE LA English DT Article ID PHENOTYPIC PLASTICITY; UNITED-STATES; POPULATION DIFFERENTIATION; LOCAL ADAPTATION; TRADE-OFFS; CHAMERION-ANGUSTIFOLIUM; SEED TRANSFER; GENECOLOGY; ZONES; RESTORATION AB Few studies have assessed how ploidy type within a species affects genetic variation among populations in relation to source climates. Basin wildrye (Leymus cinereus (Scribn. & Merr.) A. Love) is a large bunchgrass common in the intermountain Western U.S. found in both octoploid and tetraploid types. In common gardens at two sites over two years differences in both ploidy type and genetic variation within ploidy were observed in phenology, morphology, and production traits on 57 octoploid and 52 tetraploid basin wildrye from the intermountain Western U.S. (P<0.01). Octoploids had larger leaves, longer culms, and greater crown circumference than tetraploids but the numerical ranges of plant traits and their source climates overlapped between ploidy types. Still, among populations octoploids often had greater genetic variation for traits and occupied more diverse climates than tetraploids. Genetic variation for both ploidy types was linked to source climates in canonical correlation analysis, with the first two variates explaining 70% of the variation. Regression of those canonical variates with seed source climate variables produced models that explained 64% and 38% of the variation, respectively, and were used to map 15 seed zones covering 673258 km(2). Utilization of these seed zones will help ensure restoration with adaptive seed sources for both ploidy types. The link between genetic traits and seed source climates suggests climate driven natural selection and adaptive evolution in basin wildrye. The more diverse climates occupied by octoploids and higher trait variation suggests a higher capacity for ecological differentiation than tetraploids in the intermountain Western U.S. C1 [Johnson, R. C.] ARS, Plant Germplasm Res & Testing, USDA, Pullman, WA USA. [Vance-Borland, Ken] Conservat Planning Inst, Corvallis, OR USA. RP Johnson, RC (reprint author), ARS, Plant Germplasm Res & Testing, USDA, Pullman, WA USA. EM rcjohnson@wsu.edu FU United States Department of Agriculture-Agriculture Research Service; Bureau of Land Management; Great Basin Native Plant Project FX This work was funded by United States Department of Agriculture-Agriculture Research Service, Bureau of Land Management, and Great Basin Native Plant Project. NR 54 TC 1 Z9 1 U1 5 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 16 PY 2016 VL 11 IS 2 AR e0148982 DI 10.1371/journal.pone.0148982 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3BP UT WOS:000371219000039 PM 26881894 ER PT J AU Silva, CJ Erickson-Beltran, ML Dynin, IC AF Silva, Christopher J. Erickson-Beltran, Melissa L. Dynin, Irina C. TI Covalent Surface Modification of Prions: A Mass Spectrometry-Based Means of Detecting Distinctive Structural Features of Prion Strains SO BIOCHEMISTRY LA English DT Article ID TRANSMISSIBLE MINK ENCEPHALOPATHY; SMALL-MOLECULE REAGENTS; SCRAPIE AGENT; MOUSE SCRAPIE; PROTEIN PRP; AMINO-ACID; HAMSTERS; METHIONINE; OXIDATION; CONFORMATIONS AB Prions (PrPSc) are molecular pathogens that are able to convert the isosequential normal cellular prion protein (PrPC) into a prion. The only demonstrated difference between PrPC and PrPSc is conformational: they are isoforms. A given host can be infected by more than one kind or strain of prion. Five strains of hamster-adapted scrapie [Sc237 (=263K), drowsy, 139H, 22AH, and 22CH] and recombinant PrP were reacted with five different concentrations (0, 1, 5, 10, and 20 mM) of reagent (N-hydroxysuccinimide ester of acetic acid) that acetylates lysines. The extent of lysine acetylation was quantitated by mass spectrometry. The lysines in rPrP react similarly. The lysines in the strains react differently from one another in a given strain and react differently when strains are compared. Lysines in the C-terminal region of prions have different strain dependent reactivity. The results are consistent with a recently proposed model for the structure of a prion. This model proposes that prions are composed of a four-rung beta-solenoid structure comprised of four beta-sheets that are joined by loops Variation in the amino acid composition of the loops and beta-sheet structures is thought to result in and turns of amino acids. different strains of prions. C1 [Silva, Christopher J.; Erickson-Beltran, Melissa L.; Dynin, Irina C.] USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Silva, CJ (reprint author), USDA, ARS, 800 Buchanan St, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov OI Silva, Christopher/0000-0003-4521-6377 NR 52 TC 1 Z9 1 U1 1 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD FEB 16 PY 2016 VL 55 IS 6 BP 894 EP 902 DI 10.1021/acs.biochem.5b01068 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DE2JO UT WOS:000370453800007 PM 26786805 ER PT J AU Smith, AD Refsum, H Selhub, J Rosenberg, IH AF Smith, A. David Refsum, Helga Selhub, Jacob Rosenberg, Irwin H. TI FOLIC ACID FORTIFICATION FOR EUROPE? Decision on folic acid fortification in Europe must consider both risks and benefits SO BMJ-BRITISH MEDICAL JOURNAL LA English DT Letter ID NEURAL-TUBE DEFECTS; VITAMIN-B-12 STATUS; CANCER INCIDENCE; OLDER-PEOPLE; FOLATE; POPULATION; DEFICIENCY C1 [Smith, A. David] Univ Oxford, Dept Pharmacol, S Parks Rd, Oxford OX1 3QT, England. [Refsum, Helga] Univ Oslo, Inst Basic Med Sci, Dept Nutr, Oslo, Norway. [Selhub, Jacob] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rosenberg, Irwin H.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Smith, AD (reprint author), Univ Oxford, Dept Pharmacol, S Parks Rd, Oxford OX1 3QT, England. EM david.smith@pharm.ox.ac.uk NR 19 TC 3 Z9 3 U1 1 U2 4 PU BMJ PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1756-1833 J9 BMJ-BRIT MED J JI BMJ-British Medical Journal PD FEB 16 PY 2016 VL 352 AR i734 DI 10.1136/bmj.i734 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA DE6EW UT WOS:000370727500002 PM 26883421 ER PT J AU Majumdar, R Barchi, B Turlapati, SA Gagne, M Minocha, R Long, S Minocha, SC AF Majumdar, Rajtilak Barchi, Boubker Turlapati, Swathi A. Gagne, Maegan Minocha, Rakesh Long, Stephanie Minocha, Subhash C. TI Glutamate, Ornithine, Arginine, Proline, and Polyamine Metabolic Interactions: The Pathway Is Regulated at the Post-Transcriptional Level SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Arabidopsis thaliana; arginine; gamma-aminobutyric acid; glutamate; ornithine; polyamines; proline; quantitative polymerase chain reaction ID AMINO-ACIDS; ARABIDOPSIS-THALIANA; PUTRESCINE BIOSYNTHESIS; GENETIC MANIPULATION; DECARBOXYLASE CDNA; GABA-METABOLISM; POPLAR CELLS; PLANTS; EXPRESSION; NITROGEN AB The metabolism of glutamate into ornithine, arginine, proline, and polyamines is a major network of nitrogen-metabolizing pathways in plants, which also produces intermediates like nitric oxide, and gamma-aminobutyric acid (GABA) that play critical roles in plant development and stress. While the accumulations of intermediates and the products of this network depend primarily on nitrogen assimilation, the overall regulation of the interacting sub-pathways is not well understood. We tested the hypothesis that diversion of ornithine into polyamine biosynthesis (by transgenic approach) not only plays a role in regulating its own biosynthesis from glutamate but also affects arginine and proline biosynthesis. Using two high putrescine producing lines of Arabidopsis thaliana (containing a transgenic mouse ornithine decarboxylase gene), we studied the: (1) effects of exogenous supply of carbon and nitrogen on polyamines and pools of soluble amino acids; and, (2) expression of genes encoding key enzymes in the interactive pathways of arginine, proline and GABA biosynthesis as well as the catabolism of polyamines. Our findings suggest that: (1) the overall conversion of glutamate to arginine and polyamines is enhanced by increased utilization of ornithine for polyamine biosynthesis by the transgene product; (2) proline and arginine biosynthesis are regulated independently of polyamines and GABA biosynthesis; (3) the expression of most genes (28 that were studied) that encode enzymes of the interacting sub-pathways of arginine and GABA biosynthesis does not change even though overall biosynthesis of Orn from glutamate is increased several fold; and (4) increased polyamine biosynthesis results in increased assimilation of both nitrogen and carbon by the cells. C1 [Majumdar, Rajtilak; Barchi, Boubker; Turlapati, Swathi A.; Gagne, Maegan; Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Turlapati, Swathi A.; Minocha, Rakesh; Long, Stephanie] US Forest Serv, USDA, No Res Stn, Durham, NH USA. [Majumdar, Rajtilak] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. [Gagne, Maegan] Univ Wisconsin, Dept Bot, Madison, WI USA. RP Minocha, SC (reprint author), Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. EM sminocha@unh.edu FU New Hampshire Agricultural Experiment Station; USDA National Institute of Food and Agriculture (McIntire-Stennis) [NH00062, NH00076-M] FX Partial funding was provided by the New Hampshire Agricultural Experiment Station. This is Scientific Contribution Number 2608. This work was supported by the USDA National Institute of Food and Agriculture (McIntire-Stennis) Project (NH00062 and NH00076-M). NR 84 TC 7 Z9 7 U1 6 U2 27 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 16 PY 2016 VL 7 AR 78 DI 10.3389/fpls.2016.00078 PG 17 WC Plant Sciences SC Plant Sciences GA DD7SC UT WOS:000370123400001 PM 26909083 ER PT J AU Obenland, D Cantwell, M Lobo, R Collin, S Sievert, J Arpaia, ML AF Obenland, David Cantwell, Marita Lobo, Ramiro Collin, Sue Sievert, Jim Arpaia, Mary Lu TI Impact of storage conditions and variety on quality attributes and aroma volatiles of pitahaya (Hylocereus spp.) SO SCIENTIA HORTICULTURAE LA English DT Article DE Sensory; Antioxidant; Betacyanin; Sugars; Organic acids ID RED PITAYA; TEMPERATURE; POLYRHIZUS; ANTIOXIDANT; UNDATUS; FRUITS; FLAVOR AB Pitahaya are increasing in popularity but knowledge on the effects of storage and the potential impact of variety on subsequent quality following storage is incomplete, particularly in terms of the potential effects on sensory acceptability. In this study six varieties of pitahaya, having white, pink, and red internal flesh coloration, were harvested and evaluated for sensory and quality attributes at harvest and following storage for two weeks at either 5 degrees C or 10 degrees C. Storage did not influence overall visual liking or color of the external portion of the fruit as discerned by the panelists. Alterations in the internal flesh color, such as a slight darkening, were noted to occur by instrumental measurement, but these differences were not noticeable to the panelists. Losses in sugars and acids occurred in the flesh during storage at both storage temperatures and were related to declines in fructose, glucose and malic acid. In contrast, antioxidant activity was reduced by storage at 5 degrees C but was unchanged at 10 degrees C, with betacyanin concentration not differing from that determined at harvest. Aldehydes were the most abundant aroma volatiles detected in juice from the flesh, with storage increasing total aldehyde amount, particularly at 10 degrees C. Total alcohols, on the other hand, were reduced by storage, the amount of reduction not dependent on storage temperature. Regardless of the storage-induced changes in the various components measured, panelists did not report any significant differences in overall liking, flavor, sweetness, tartness or texture. There were substantial varietal differences in sensory and quality attributes, regardless of the impact of storage. 'Cebra' had very low sweetness relative to the other varieties and had low flavor and overall liking scores. Panelist perception of flavor quality was most closely linked with varietal differences in SSC and TA, although there were clear differences in aroma volatiles as well. Mexicana, a white-fleshed variety, had the lowest antioxidant activity, corresponding to low amounts of the red betacyanin pigments. The study indicated that storage, and particularly that at 10 degrees C, caused a variety of measureable changes in a range of pitahaya quality parameters but that these changes did not substantially alter the sensory quality of the fruit. Further research is needed to determine whether or not varietal or storage-induced differences in aroma volatile content have an impact on pitahaya flavor. Published by Elsevier B.V. C1 [Obenland, David] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Cantwell, Marita] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Lobo, Ramiro] Univ Calif Cooperat Extens, San Diego, CA 92123 USA. [Collin, Sue; Sievert, Jim; Arpaia, Mary Lu] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. RP Obenland, D (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM david.obenland@ars.usda.gov NR 18 TC 0 Z9 1 U1 14 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD FEB 16 PY 2016 VL 199 BP 15 EP 22 DI 10.1016/j.scienta.2015.12.021 PG 8 WC Horticulture SC Agriculture GA DD7GZ UT WOS:000370093000003 ER PT J AU Canon-Franco, WA Lopez-Orozco, N Christoff, AU de Castilho, CS de Araujo, FAP Verma, SK Dubey, JP Soares, RM Gennari, SM AF Alberto Canon-Franco, William Lopez-Orozco, Natalia Christoff, Alexandre Uarth de Castilho, Camila Schlieper Pacheco de Araujo, Flavio Antonio Verma, Shiv Kumar Dubey, J. P. Soares, Rodrigo Martins Gennari, Solange Maria TI Molecular and morphologic characterization of Sarcocystis felis (Apicomplexa: Sarcocystidae) in South American wild felids from Brazil SO VETERINARY PARASITOLOGY LA English DT Article DE Felidae; Wild cats; Brazil; Sarcocystis ID HAMMONDIA-HEYDORNI; INFECTION; CATS; OOCYSTS; RUFUS AB Wild felids are thought to share parasites with domestic cats. However, little is known of the coccidian parasites of wild felids. We investigated the presence of Sarcocystis spp. in tissues of 6 species of 90 Neotropical small felids killed in road accidents in the state of Rio Grande do Sul, Brazil by using microscopic and molecular techniques. Formalin-fixed tissues from 28 fends were examined, and Sarcocystis felis-like sarcocysts were detected in 4 wild cats (2 Puma yagouaroundi and 2 Leopardus guttulus). By transmission electron microscopy, sarcocysts from a P. yagouaroundi were identical to S. felis from domestic cats in the USA. Direct sequencing of PCR amplicons resulted the unambiguous sequences of the ITS-1 region from 18 of the 31 PCR positive wild cats; 5 sequences from each P. yagouaroundi, and Leopardus geoffroyi, 4 sequences from L. guttulus, and 2 sequences from each Leopardus wiedii, and Leopardus colocolo. Sequences analysis of ITS-1 region revealed the highest identiy (97-99%) with that of previously describe isolates of S. felis from domestic cats in the USA and identified them as S. felis. Tissues of 1 Leopardus pardalis tested by PCR and histology were negative. The phylogenetic relationship indicated that S. felis is quite different to species which employ opossums as their definitive host. This is the first report of S. felis infection in small wild felids from Brazil. (C) 2016 Elsevier B.V. All rights reserved. C1 [Alberto Canon-Franco, William] Univ Caldas, Fac Ciencias Agropecuarias, Dept Salud Anim, Calle 65 26-10, Manizales, Colombia. [Alberto Canon-Franco, William; Lopez-Orozco, Natalia; Soares, Rodrigo Martins; Gennari, Solange Maria] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecn, Av Prof Dr Orlando Marques Paiva 87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. [Lopez-Orozco, Natalia] Univ Tecnol Pereira, Fac Ciencias Salud, Dept Ciencias Basicas, Grpo Infecc & Inmunidad, AA 097, La Julita, Pereira, Colombia. [Christoff, Alexandre Uarth] Univ Luterana Brasil, Dept Biol, Museu Ciencias Nat, Av Farroupilha 8001, BR-92425900 Canoas, RS, Brazil. [de Castilho, Camila Schlieper] Univ Sao Paulo, Inst Biociencias, Dept Ecol, Rua Mateo 321 Tray 14, BR-05508090 Sao Paulo, SP, Brazil. [Pacheco de Araujo, Flavio Antonio] Univ Fed Rio Grande do Sul, Fac Vet, Dept Patol & Clin Vet, Av Bento Goncalves 9090, BR-91540000 Porto Alegre, RS, Brazil. [Verma, Shiv Kumar; Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. RP Gennari, SM (reprint author), Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecn, Av Prof Dr Orlando Marques Paiva 87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. EM sgennari@usp.br RI Soares, Rodrigo/E-8747-2015 FU CAPES [Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/Coordination for the Improvement of Higher Education Personnel]; Fundacao de Apoio a Pesquisa do Estado de Sao Paulo/Sao Paulo Research Foundation, Brazil [2012/25180-9]; CNPq [Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/National Counsel of Technological and Scientific Development]; FAPESP [Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/Sao Paulo Research Foundation] [2010/52308-0] FX The authors thank the following institutions that allowed the use of animal samples from their collections: Museum of Science and Technology of the Pontifical Catholic University of Rio Grande do Sul (PUCRS), Federal University of Rio Grande do Sul Laboratory of Cytogenetics and Molecular Evolution (UFRGS), Zoobotanic Foundation of Rio Grande do Sul (FZB), Museum of Natural Sciences of the University of Caxias do Sul (UCS), and the Science Museum of the Lutheran University of Brazil (ULBRA). WAC-F thanks CAPES [Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/Coordination for the Improvement of Higher Education Personnel] for providing a scholarship (PEC-PG: Programa de Estudante-Convenio de Pos-Graduacao [Scholarship Program for Fulltime Postgraduate Courses]) and postdoctoral fellowship funding (process number 2012/25180-9) Fundacao de Apoio a Pesquisa do Estado de Sao Paulo/Sao Paulo Research Foundation, Brazil. SMG and RMS thank CNPq [Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/National Counsel of Technological and Scientific Development] for awarding a research productivity grant. The authors also thank Mr. Efrain Perez and Joseph Madary, Joint Pathology Center, Veterinary Services, U.S. Army, Silver Spring, Maryland for excellent technical help with electron microscopy. This study was financed in part by FAPESP [Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/Sao Paulo Research Foundation] (process number 2010/52308-0). NR 16 TC 0 Z9 0 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB 15 PY 2016 VL 217 BP 15 EP 20 DI 10.1016/j.vetpar.2015.12.025 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DE2LK UT WOS:000370458600004 PM 26827854 ER PT J AU Romero-Salas, D Mira, A Mosqueda, J Garcia-Vazquez, Z Hidalgo-Ruiz, M Vela, NAO de Leon, AAP Florin-Christensen, M Schnittger, L AF Romero-Salas, Dora Mira, Anabela Mosqueda, Juan Garcia-Vazquez, Zeferino Hidalgo-Ruiz, Mario Ortiz Vela, Noot Aditya Perez de Leon, Adalberto Angel Florin-Christensen, Monica Schnittger, Leonhard TI Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field SO VETERINARY PARASITOLOGY LA English DT Article DE Babesia bovis; Babesia bigemina; Indirect fluorescent-antibody test (IFAT); Nested PCR; Epidemiology; Bovines; Water buffaloes ID ANAPLASMA-MARGINALE; CATTLE; ASSAY; THEILERIA; ANTIGENS; CARRIERS AB Babesia bovis and Babesia bigemina are causative agents of bovine babesiosis, a tick-borne disease of cattle in tropical and subtropical regions. Babesia spp. infection adversely affects cattle health and can be fatal resulting in considerable economic loss worldwide. Under endemic stability conditions, herds contain high numbers of chronically infected, asymptomatic carrier animals, in which no parasitemia is detected by microscopic blood smear examination. In addition to bovines, also water buffaloes are infected by both Babesia spp. commonly leading to a subclinical infection. The infection rate (by nPCR) and herd exposure (by IFAT) of bovines and water buffaloes reared under similar field conditions in an area of endemic stability were determined and compared. In order to optimize direct parasite detection, highly sensitive nPCR assays were developed and applied, allowing the detection of as little as 0.1 fg DNA of each Babesia pathogen. Significantly lower percentages (p < 0.001) of seropositive water buffaloes compared to bovines were observed for B. bovis (71.4% vs. 98%) and B. bigemina (85% vs. 100%). Interestingly, in comparison, differences noticed between water buffaloes and bovines were considerably larger with direct parasite detection by nPCR (16.2% vs. 82.3% and 24% vs. 94.1% for B. bovis and B. bigemina, respectively). As expected, bovines subjected to monthly acaricide applications exhibited a significant lower infection rate as determined by nPCR than bovines not subjected to these measures (B. bovis 333% vs. 90.7%, p < 0.001; B. bigemina 80% vs. 96.5%, p < 0.001, for treated vs. untreated animals). Interestingly no differences between these groups were observed with respect to seropositivity, suggesting similar rates of parasite exposure (B. bovis 100% vs. 97.7%, p < 0.001; B. bigemina 100% vs. 100%, p < 0.001). Importantly, a significantly higher number of water buffaloes as determined by nPCR were infected when reared jointly with bovines not subjected to tick control than when reared jointly with bovines subjected to tick control (B. bovis 31.6% vs. 9.5%, p < 0.01; B. bigemina 42.1% vs. 9.5%, p < 0.01, for water buffaloes reared with untreated vs. treated bovines) and/or when reared without bovines (B. bovis 31.6% vs. 11.6%, p < 0.01; B. bigemina 42.1% vs. 20%, p < 0.01). An accumulation of seropositivity and a decline of infection rates were observed in older animals, while differences observed with regard to gender may warrant further investigation. In summary, our findings suggest that water buffaloes are much more capable to limit or eliminate Babesia infection, possibly due to a more capable immune defense. Furthermore, an increased Babesia spp. parasite reservoir of bovines seems to increase the infection rate of water buffaloes when both are reared on the same pasture. (C) 2016 Elsevier B.V. All rights reserved. C1 [Romero-Salas, Dora; Ortiz Vela, Noot Aditya] Univ Veracruzana, Fac Med Vet & Zootecnia, Lab Parasitol, Circunvalac & Yanez S-N, Xalapa 91710, Veracruz, Mexico. [Mira, Anabela; Florin-Christensen, Monica; Schnittger, Leonhard] INTA Castelar, CICVyA, Inst Patobiol, Los Reseros & Nicolas Repetto S-N, RA-1686 Hurlingham, Buenos Aires, Argentina. [Mosqueda, Juan; Hidalgo-Ruiz, Mario] Univ Autonoma Queretaro, Fac Ciencias Nat, Campus Juriquilla, Queretaro 76230, Mexico. [Garcia-Vazquez, Zeferino] Ctr Nacl Invest Disciplinaria Parasitol Vet, INIFAP, Jiutepec 6225, Morelos, Mexico. [Perez de Leon, Adalberto Angel] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX USA. [Florin-Christensen, Monica; Schnittger, Leonhard] Consejo Nacl Invest Cient & Tecn, C1033AAJ, RA-1033 Buenos Aires, DF, Argentina. RP Schnittger, L (reprint author), INTA Castelar, CICVyA, Inst Patobiol, Los Reseros & Nicolas Repetto S-N, RA-1686 Hurlingham, Buenos Aires, Argentina. EM schnittger.leonhard@inta.gob.ar FU National Research Council of Argentina (CONICET); National Institutes of Agricultural Technology (INTA) [CORRI-1243106]; project Sep-Conacyt [167129] FX The authors want to thank the financial contributions of the National Research Council of Argentina (CONICET, salaries of AM, MFC and LS), the National Institutes of Agricultural Technology (INTA, CORRI-1243106), and the project Sep-Conacyt 167129. The assistance of and collaboration with the Laboratory of Parasitology of the Faculty of Veterinary Medicine and Zootechnics of the University of Veracruz, the CENID-PAVET of INIFAP, and the life stock and buffalo producers of the Center and the South of Veracruz, Mexico are also gratefully acknowledged. NR 31 TC 1 Z9 1 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB 15 PY 2016 VL 217 BP 101 EP 107 DI 10.1016/j.vetpar.2015.12.030 PG 7 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DE2LK UT WOS:000370458600019 PM 26827869 ER PT J AU da Cunha, MJR Cury, MC Santin, M AF Rodrigues da Cunha, Maria Julia Cury, Marcia Cristina Santin, Monica TI Widespread presence of human-pathogenic Enterocytozoon bieneusi genotypes in chickens SO VETERINARY PARASITOLOGY LA English DT Article DE Brazil; Birds; Enterocytozoon bieneusi; Genotypes; PCR ID MOLECULAR CHARACTERIZATION; 1ST DETECTION; MICROSPORIDIA; PIGEONS; SEQUENCE; OUTBREAK; SPORES; BIRDS; LIMA; PERU AB A total of 151 fecal specimens from chickens were randomly collected from local markets in Uberlandia and Belo Horizonte in the state of Minas Gerais, Brazil, to evaluate the presence of Enterocytozoon bieneusi by polymerase chain reaction (PCR). Enterocytozoon bieneusi was identified in 24 fecal samples (15.9%). This represents the first report of E. bieneusi in chickens in Brazil. All PCR-positive specimens were sequenced and 4 genotypes were identified, Peru 6, Peru 11, Type IV, and D. All four genotypes have previously been reported as human pathogens and are potentially zoonotic. Our results demonstrate that human-pathogenic E. bieneusi genotypes are present in chickens in Brazil, corroborating their potential role as a source of human infection and environmental contamination. Published by Elsevier B.V. C1 [Rodrigues da Cunha, Maria Julia; Santin, Monica] ARS, Environm Microbial & Food Safety Lab, USDA, Bldg 173,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Rodrigues da Cunha, Maria Julia; Cury, Marcia Cristina] Univ Fed Uberlandia, Inst Ciencias Biomed, Lab Parasitol, Ave Para,1720,Campus Umuarama, BR-38400902 Uberlandia, MG, Brazil. [Rodrigues da Cunha, Maria Julia] Minist Educ Brazil, CAPES Fdn, Caixa Postal 250, BR-70040020 Brasilia, DF, Brazil. RP Santin, M (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Bldg 173,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM monica.santin-duran@ars.usda.gov FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [99999.009537/2014-07] FX This study was supported by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), process number 99999.009537/2014-07. NR 30 TC 1 Z9 1 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD FEB 15 PY 2016 VL 217 BP 108 EP 112 DI 10.1016/j.vetpar.2015.12.019 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DE2LK UT WOS:000370458600020 PM 26827870 ER PT J AU Basche, AD Archontoulis, SV Kaspar, TC Jaynes, DB Parkin, TB Miguez, FE AF Basche, Andrea D. Archontoulis, Sotirios V. Kaspar, Thomas C. Jaynes, Dan B. Parkin, Timothy B. Miguez, Fernando E. TI Simulating long-term impacts of cover crops and climate change on crop production and environmental outcomes in the Midwestern United States SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Climate change; Cereal rye cover crop; Maize; Soybean; Greenhouse gas; Soil carbon; Soil erosion; APSIM; Midwest United States ID NITROUS-OXIDE EMISSIONS; EASTERN DARLING-DOWNS; ORGANIC-CARBON; SOIL-EROSION; MANAGEMENT-PRACTICES; CORN RESPONSE; TILE DRAINAGE; WINTER RYE; SYSTEMS; TILLAGE AB It is critical to evaluate conservation practices that protect soil and water resources from climate change in the Midwestern United States, a region that produces one-quarter of the world's soybeans and one-third of the world's maize. An over-winter cover crop in a maize-soybean rotation offers multiple potential benefits that can reduce the impacts of higher temperatures and more variable rainfall; some of the anticipated changes for the Midwest. In this experiment we used the Agricultural Production Systems sIMulator (APSIM) to understand how winter rye cover crops impact crop production and environmental outcomes, given future climate change. We first tested APSIM with data from a long-term maize-soybean rotation with and without winter rye cover crop field site. Our modeling work predicted that the winter rye cover crop has a neutral effect on maize and soybean yields over the 45 year simulation period but increases in minimum and maximum temperatures were associated with reduced yields of 1.6-2.7% by decade. Soil carbon decreased in both the cover crop and no cover crop simulations, although the cover crop is able to significantly offset (3% less loss over 45 years) this decline compared to the no cover crop simulation. Our predictions showed that the cover crop led to an 11-29% reduction in erosion and up to a 34% decrease in nitrous oxide emissions (N2O). However, the cover crop is unable to offset future predicted yield declines and does not increase the overall carbon balance relative to current soil conditions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Basche, Andrea D.; Archontoulis, Sotirios V.; Miguez, Fernando E.] Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50011 USA. [Kaspar, Thomas C.; Jaynes, Dan B.; Parkin, Timothy B.] USDA ARS, Natl Lab Agr & Environm, 2150 Pammel Dr, Ames, IA 50014 USA. RP Miguez, FE (reprint author), Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50011 USA. EM femiguez@iastate.edu FU USDA-NIFA [2011-68002-30190]; Leopold Center for Sustainable Agriculture award [E2013-19]; Iowa State Agronomy Department Research Training Fellowship; National Science Foundation [DGE0947929] FX This research is part of a regional collaborative project supported by the USDA-NIFA, Award No. 2011-68002-30190, "Cropping Systems Coordinated Agricultural Project: Climate Change, Mitigation, and Adaptation in Corn-based Cropping Systems." Project Web site: sustainablecorn.org. This research was also financially supported by the Leopold Center for Sustainable Agriculture award number E2013-19, the Iowa State Agronomy Department Research Training Fellowship and the National Science Foundation funded GK-12 program award number DGE0947929. The authors would also like to thank the following people for their assistance with data collection: Keith Kohler, Ben Knutson, Otis Smith, Richard Hartwig, Wendy Borja and Jacob House. The authors would like to further thank the contributions of Andrew Lenssen, Lisa Schulte Moore and Michael Castellano for their review of this manuscript. NR 99 TC 4 Z9 4 U1 26 U2 86 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD FEB 15 PY 2016 VL 218 BP 95 EP 106 DI 10.1016/j.agee.2015.11.011 PG 12 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DC8IM UT WOS:000369463100011 ER PT J AU Rogovska, N Laird, DA Karlen, DL AF Rogovska, Natalia Laird, David A. Karlen, Douglas L. TI Corn and soil response to biochar application and stover harvest SO FIELD CROPS RESEARCH LA English DT Article DE Biochar; Residue; Bioenergy; Soil quality; Continuous corn ID ORGANIC-MATTER; RESIDUE REMOVAL; HYDRAULIC-PROPERTIES; CHEMICAL-PROPERTIES; SOYBEAN ROTATION; TILLAGE; MANAGEMENT; QUALITY; NITROGEN; AMENDMENT AB Soil biochar amendments are hypothesized to help mitigate potential negative soil quality effects of harvesting crop residues for bioenergy production. This study quantified effects of a onetime biochar application on crop yields and changes in soil properties at a Midwestern U.S.A. site where corn (Zeamays) stover is being harvested. The long-term experiment was established in Boone County, Iowa by application of 0, 9.8, and 18.4 Mg ha(-1) of <0.64 cm hardwood biochar, in the fall of 2007. Soil properties were measured in summer 2010, three years after the biochar application and following two stover harvests (0, similar to 50 and similar to 90% of above ground residue). Fertilization rates were adjusted to account for removal of nutrients with residue. Corn and biomass yields were measured for the 2008-2012 crop years. Biochar applications increased total carbon (TC) of soils by about 0.5% and soil pH by about 0.5 pH units but had no significant effect on total nitrogen (TN), soil test nutrient concentrations, bulk density (BD), aggregate stability or effective cation exchange capacity (ECEC). Residue removal had no significant effect on TC, TN, ECEC, or soil test nutrients except for Mehlich III extractable Zn and S concentrations. The 90% residue removal treatment significantly reduced wet aggregate mean weight diameter by 13% compared to no residue harvest. During the first five years of the study, the 50 and 90% residue removal treatments significantly increased corn grain yields by 1.4 Mg ha(-1), averaged across all biochar treatments. When analyzed on an annual basis, biochar had no significant effect on grain yield, but when averaged across years, the 18.4 Mg ha(-1) biochar application rate significantly increased yields by 0.49 Mg ha(-1) for the zero residue removal treatment but not for the 50% and 90% residue removal treatments. Residue removal increased yield to a greater extent during years of stress due to either excess moisture (2008 and 2010) or drought (2012). There was no yield effect in 2011, a year with favorable climatic conditions. The results suggest that during the first five years of study, residue removal in general had positive effect on yield of continues corn. Application of biochar at the high rate increased yield in the plots with 0% residue removal. (C) 2015 Elsevier B.V. All rights reserved. C1 [Rogovska, Natalia; Laird, David A.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Karlen, Douglas L.] USDA ARS, Natl Lab Agric Environ, 2110 Univ Blvd, Ames, IA 50011 USA. RP Laird, DA (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM dalaird@iastate.edu FU USDA-Agricultural Research Service (ARS) [3625-12000-013-D]; Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2011-68005-30411]; National Science Foundation [EPS-1101284] FX We thank all members of the REAP team for their contributions to this collaborative effort and we specifically thank Pierce Fleming, Larry Pellack and Rich Hartwig for their help with the field and laboratory work. Funding for this research was provided by the USDA-Agricultural Research Service (ARS) through Project 3625-12000-013-D, by Agriculture and Food Research Initiative Competitive Grant no. 2011-68005-30411 from the USDA National Institute of Food and Agriculture, and by the National Science Foundation under Grant Number EPS-1101284. NR 62 TC 2 Z9 2 U1 17 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 15 PY 2016 VL 187 BP 96 EP 106 DI 10.1016/j.fcr.2015.12.013 PG 11 WC Agronomy SC Agriculture GA DC8LB UT WOS:000369470000009 ER PT J AU Aninbon, C Jogloy, S Vorasoot, N Nuchadomrong, S Senawong, T Holbrook, CC Patanothai, A AF Aninbon, Chorkaew Jogloy, Sanun Vorasoot, Nimitr Nuchadomrong, Suporn Senawong, Thanaset Holbrook, C. Corley Patanothai, Aran TI Effect of mid season drought on phenolic compounds in peanut genotypes with different levels of resistance to drought SO FIELD CROPS RESEARCH LA English DT Article DE Groundnut; Water deficit; Secondary metabolites; Polyphenol ID STRESS; TOLERANCE; ACIDS; L.; ANTIOXIDANTS; POLYPHENOLS; LEAVES; SEEDS AB Drought is a major constraint in peanut production. Drought not only reduces pod yield but also may affect phenolic compounds in peanut. This experiment was conducted for two years under field conditions. Soil moisture levels (field capacity and mid season drought stress) were assigned as main plots, and five peanut genotypes were assigned as sub plots. Peanut seeds were analyzed for phenolic compounds at harvest. Leaves and stems were also analyzed at 30 and 60 days after planting (DAP), and at harvest. Water regimes did not result in significant differences for phenolic compounds in seeds, whereas peanut genotypes were significantly different for this trait. Drought increased phenolic contents in leaves at 60 DAP and phenolic contents were reduced during the recovery period. The production of leaf phenolic compounds differed among genotypes. Mid season drought increased phenolic compounds in stems during the drought period and levels of phenolic production were different depending on peanut genotype. Breeding for high phenolic content and drought tolerance in peanut should be possible because mid season drought did not affect phenolic content in seeds. (C) 2015 Elsevier B.V. All rights reserved. C1 [Aninbon, Chorkaew; Jogloy, Sanun; Vorasoot, Nimitr; Patanothai, Aran] Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. [Nuchadomrong, Suporn; Senawong, Thanaset] Khon Kaen Univ, Dept Biochem, Fac Sci, Khon Kaen 40002, Thailand. [Holbrook, C. Corley] USDA, Crop Genet & Breeding Res, Tifton, GA USA. RP Jogloy, S (reprint author), Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. EM sjogloy@gmail.com FU Royal Golden Jubilee Ph.D. program [PHD/0003/2554]; Khon Kaen University [PHD/0003/2554]; peanut and Jerusalem Artichoke Improvement for functional Food Research Group, Khon Kaen University; Higher Education Promotion and National Research University Project of Thailand of the Office of Higher Education Commission through the Food and Functional Food Research Cluster of Khon Kaen University; Thailand Research Fund (TRF) [RTA 5880003, IRG 5780003]; Khon Kaen University (KKU); Faculty of Agriculture KKU; Plant Breeding Research Center for Sustainable Agriculture FX The authors are grateful for the jointed financial support of the Royal Golden Jubilee Ph.D. program and Khon Kaen University (grant no. PHD/0003/2554), the peanut and Jerusalem Artichoke Improvement for functional Food Research Group, Khon Kaen University, The Higher Education Promotion and National Research University Project of Thailand of the Office of Higher Education Commission were also acknowledged for funding support through the Food and Functional Food Research Cluster of Khon Kaen University, Plant Breeding Research Center for Sustainable Agriculture, the Thailand Research Fund (TRF) for providing financial supports to this research through the Senior Research Scholar Project of Professor Dr. Sanun Jogloy (Project no. RTA 5880003). Acknowledgment is extended to the Thailand Research Fund (TRF, Project no. IRG 5780003), Khon Kaen University (KKU) and the Faculty of Agriculture KKU for providing financial support for manuscript preparation activities. NR 32 TC 0 Z9 0 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 15 PY 2016 VL 187 BP 127 EP 134 DI 10.1016/j.fcr.2015.12.016 PG 8 WC Agronomy SC Agriculture GA DC8LB UT WOS:000369470000012 ER PT J AU Scott, JH Thompson, MP Gilbertson-Day, JW AF Scott, Joe H. Thompson, Matthew P. Gilbertson-Day, Julie W. TI Examining alternative fuel management strategies and the relative contribution of National Forest System land to wildfire risk to adjacent homes - A pilot assessment on the Sierra National Forest, California, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Exposure and effects analysis; Fuel management; Risk source; Risk transmission; WUI ID WILDLAND-URBAN INTERFACE; UNITED-STATES; EXPOSURE; TRANSMISSION; SIMULATION; AUSTRALIA; OREGON; EXTENT AB Determining the degree of risk that wildfires pose to homes, where across the landscape the risk originates, and who can best mitigate risk are integral elements of effective co-management of wildfire risk. Developing assessments and tools to help provide this information is a high priority for federal land management agencies such as the US Forest Service (USFS) that have limited resources to invest in hazardous fuel reduction and other mitigation activities. In this manuscript we investigate the degree to which fuel management practices on USFS land can reduce wildfire exposure to human communities. We leverage wildfire simulation with spatial risk analysis techniques and examine a range of hypothetical fuel treatment scenarios on a landscape encompassing the Sierra National Forest in California, USA. Results suggest that treating USFS land does little to reduce overall wildland urban interface (WUI) exposure across the landscape. A treatment scenario that focused on treating defensible space near homes was by far the most efficient at reducing WUI exposure, including exposure transmitted from USFS lands. Findings highlight potential tradeoffs and raise questions as to what other land management objectives fuel treatments on federal lands might be able to more cost-effectively achieve relative to WUI protection. Site-specific risk-based analyses can help elucidate these tradeoffs and opportunities. Published by Elsevier B.V. C1 [Scott, Joe H.; Gilbertson-Day, Julie W.] Pyrologix LLC, 111 N Higgins Ave,Suite 404, Missoula, MT 59802 USA. [Thompson, Matthew P.] US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. RP Thompson, MP (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. EM mpthompson02@fs.fed.us FU Rocky Mountain Research Station; National Fire Decision Support Center FX The Rocky Mountain Research Station and the National Fire Decision Support Center supported this effort. NR 40 TC 1 Z9 1 U1 6 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 15 PY 2016 VL 362 BP 29 EP 37 DI 10.1016/j.foreco.2015.11.038 PG 9 WC Forestry SC Forestry GA DC9QS UT WOS:000369556700004 ER PT J AU Laflower, DM Hurteau, MD Koch, GW North, MP Hungate, BA AF Laflower, Danelle M. Hurteau, Matthew D. Koch, George W. North, Malcolm P. Hungate, Bruce A. TI Climate-driven changes in forest succession and the influence of management on forest carbon dynamics in the Puget Lowlands of Washington State, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Carbon; Climate change; LANDIS-II; Succession; Disturbance ID LANDSCAPE SIMULATION-MODEL; PACIFIC-NORTHWEST FORESTS; MANAGING FORESTS; OREGON FORESTS; UNITED-STATES; DOUGLAS-FIR; TREE GROWTH; BIOMASS; DISTURBANCE; ECOSYSTEMS AB Projecting the response of forests to changing climate requires understanding how biotic and abiotic controls on tree growth will change over time. As temperature and interannual precipitation variability increase, the overall forest response is likely to be influenced by species-specific responses to changing climate. Management actions that alter composition and density may help buffer forests against the effects of changing climate, but may require tradeoffs in ecosystem services. We sought to quantify how projected changes in climate and different management regimes would alter the composition and productivity of Puget Lowland forests in Washington State, USA. We modeled forest responses to four treatments (control, burn-only, thin-only, thin-and-burn) under five different climate scenarios: baseline climate (historical) and projections from two climate models (CCSM4 and CNRM-CM5), driven by moderate (RCP 4.5) and high (RCP 8.5) emission scenarios. We also simulated the effects of intensive management to restore Oregon white oak woodlands (Quercus garryana) for the western gray squirrel (Sciurus griseus) and quantified the effects of these treatments on the probability of oak occurrence and carbon sequestration. At the landscape scale we found little difference in carbon dynamics between baseline and moderate emission scenarios. However, by late-century under the high emission scenario, climate change reduced forest productivity and decreased species richness across a large proportion of the study area. Regardless of the climate scenario, we found that thinning and burning treatments increased the carbon sequestration rate because of decreased resource competition. However, increased productivity with management was not sufficient to prevent an overall decline in productivity under the high emission scenario. We also found that intensive oak restoration treatments were effective at increasing the probability of oak presence and that the limited extent of the treatments resulted in small declines in total ecosystem carbon across the landscape as compared to the thin-and-burn treatment. Our research suggests that carbon dynamics in this system under the moderate emission scenario may be fairly consistent with the carbon dynamics under historical climate, but that the high emission scenario may alter the successional trajectory of these forests. (C) 2015 Elsevier B.V. All rights reserved. C1 [Laflower, Danelle M.] Penn State Univ, IGDP Ecol, University Pk, PA 16802 USA. [Laflower, Danelle M.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Hurteau, Matthew D.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Koch, George W.; Hungate, Bruce A.] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Koch, George W.; Hungate, Bruce A.] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA. [North, Malcolm P.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. RP Hurteau, MD (reprint author), Univ New Mexico, MSC03 2020, Albuquerque, NM 87131 USA. EM mhurteau@unm.edu RI Hurteau, Matthew/D-2301-2009 OI Hurteau, Matthew/0000-0001-8457-8974 FU US Department of Defense's Strategic Environmental Research and Development Program (SERDP) [RC-2118] FX Funding for this research was provided by the US Department of Defense's Strategic Environmental Research and Development Program (SERDP, project RC-2118). We thank the JBLM field crew for assistance with data collection and Jeff Foster for providing data and facilitating access to field locations. We also thank M. Kaye and K. Shea for providing feedback on an earlier version of this manuscript. NR 74 TC 2 Z9 2 U1 17 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 15 PY 2016 VL 362 BP 194 EP 204 DI 10.1016/j.foreco.2015.12.015 PG 11 WC Forestry SC Forestry GA DC9QS UT WOS:000369556700020 ER PT J AU Oliver, DM Porter, KDH Pachepsky, YA Muirhead, RW Reaney, SM Coffey, R Kay, D Milledge, DG Hong, E Anthony, SG Page, T Bloodworth, JW Mellander, PE Carbonneau, PE McGrane, SJ Quilliam, RS AF Oliver, David M. Porter, Kenneth D. H. Pachepsky, Yakov A. Muirhead, Richard W. Reaney, Sim M. Coffey, Rory Kay, David Milledge, David G. Hong, Eunmi Anthony, Steven G. Page, Trevor Bloodworth, Jack W. Mellander, Per-Erik Carbonneau, Patrice E. McGrane, Scott J. Quilliam, Richard S. TI Predicting microbial water quality with models: Over-arching questions for managing risk in agricultural catchments SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article ID FECAL INDICATOR BACTERIA; ESCHERICHIA-COLI; LAND-USE; SIMULATED RAINFALL; BORNE PATHOGENS; SURFACE WATERS; CLIMATE-CHANGE; HEALTH-RISKS; ANIMAL WASTE; FLOOD RISK AB The application of models to predict concentrations of faecal indicator organisms (FIOs) in environmental systems plays an important role for guiding decision-making associated with the management of microbial water quality. In recent years there has been an increasing demand by policy-makers for models to help inform FIO dynamics in order to prioritise efforts for environmental and human-health protection. However, given the limited evidence-base on which FIO models are built relative to other agricultural pollutants (e.g. nutrients) it is imperative that the end-user expectations of FIO models are appropriately managed. In response, this commentary highlights four over-arching questions associated with: (i) model purpose; (ii) modelling approach; (iii) data availability; and (iv) model application, that must be considered as part of good practice prior to the deployment of any modelling approach to predict FIO behaviour in catchment systems. A series of short and longer-term research priorities are proposed in response to these questions in order to promote better model deployment in the field of catchment microbial dynamics. (C) 2015 The Authors. Published by Elsevier B.V. C1 [Oliver, David M.; Porter, Kenneth D. H.; Quilliam, Richard S.] Univ Stirling, Sch Nat Sci, Biol & Environm Sci, Stirling FK9 4LA, Scotland. [Pachepsky, Yakov A.; Hong, Eunmi] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Muirhead, Richard W.] AgResearch, Land & Environm, Invermay Res Ctr, Private Bag 50034, Mosgiel 9053, New Zealand. [Reaney, Sim M.; Milledge, David G.; Carbonneau, Patrice E.] Univ Durham, Dept Geog, Durham DH1 3LE, England. [Coffey, Rory] Univ Coll Dublin, Agr & Food Sci Ctr, Sch Biosyst Engn, Dublin 2, Ireland. [Kay, David] Aberystwyth Univ, Ctr Res Environm & Hlth, Aberystwyth SY23 3DB, Dyfed, Wales. [Anthony, Steven G.] ADAS Grp Ltd, HQ Pendeford House,Pendeford Business Pk, Wolverhampton WV9 5AP, W Midlands, England. [Page, Trevor] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Bloodworth, Jack W.] Cranfield Univ, Cranfield Water Sci Inst, Cranfield MK43 0AL, Beds, England. [Mellander, Per-Erik] TEAGASC, Agr Catchments Programme, Johnstown Castle, Wexford, Ireland. [McGrane, Scott J.] Univ Surrey, Dept Civil & Environm Engn, Surrey GU2 7XH, England. [Coffey, Rory] US EPA, Off Res & Dev, Washington, DC 20460 USA. RP Oliver, DM (reprint author), Univ Stirling, Sch Nat Sci, Biol & Environm Sci, Stirling FK9 4LA, Scotland. EM david.oliver@stir.ac.uk RI Coffey, Rory/G-4542-2014; OI Coffey, Rory/0000-0001-5941-0205; Quilliam, Richard/0000-0001-7020-4410; Oliver, David/0000-0002-6200-562X; Milledge, David/0000-0003-4077-4898; McGrane, Scott/0000-0002-5277-1347; Pachepsky, Yakov/0000-0003-0232-6090; Muirhead, Richard/0000-0002-0913-561X FU UK Natural Environment Research Council, 'PRACTICAL Modelling' project [NE/M005860/1] FX This work was supported by the UK Natural Environment Research Council, as part of the 'PRACTICAL Modelling' project (NE/M005860/1). We are grateful for the constructive comments of the three reviewers and the handling editor. NR 74 TC 6 Z9 6 U1 1 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 15 PY 2016 VL 544 BP 39 EP 47 DI 10.1016/j.scitotenv.2015.11.086 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC8TB UT WOS:000369491500006 PM 26657248 ER PT J AU Ibekwe, AM Ma, J Murinda, S Reddy, GB AF Ibekwe, A. M. Ma, J. Murinda, Shelton Reddy, G. B. TI Bacterial community dynamics in surface flow constructed wetlands for the treatment of swine waste SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Constructed wetland; Contaminants; Pyrosequencing; Microbial community; Swine; Wastewater ID MARSH-POND-MARSH; 16S RIBOSOMAL-RNA; WATER TREATMENT; MICROBIAL COMMUNITIES; GENETIC-MARKERS; REMOVAL; NITRIFICATION; INDICATORS; PATHOGENS; SYSTEMS AB Constructed wetlands are generally used for the removal of waste from contaminated water. In the swine production system, wastes are traditionally flushed into an anaerobic lagoon which is then sprayed on agricultural fields. However, continuous spraying of lagoon wastewater on fields can lead to high N and P accumulations in soil or lead to runoff which may contaminate surface or ground water with pathogens and nutrients. In this study, continuous marsh constructed wetland was used for the removal of contaminants from swine waste. Using pyrosequencing, we assessed bacterial composition within the wetland using principal coordinate analysis (PCoA) which showed that bacterial composition from manure influent and lagoon water were significantly different (P = 0.001) from the storage pond to the final effluent. Canonical correspondence analysis (CCA) showed that different bacterial populations were significantly impacted by ammonium - NH4 (P = 0.035), phosphate - PO43- (P = 0.010), chemical oxygen demand - COD (P = 0.0165), total solids - TS (P = 0.030), and dissolved solids - DS (P = 0.030) removal, with 54% of the removal rate explained by NH4 + PO43- according to a partial CCA. Our results showed that different bacterial groups were responsible for the composition of different wetland nutrients and decomposition process. This may be the major reason why most wetlands are very efficient in waste decomposition. Published by Elsevier B.V. C1 [Ibekwe, A. M.] USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Ma, J.] Jilin Univ, Coll Environm & Resources, Changchun 130021, Jilin Province, Peoples R China. [Murinda, Shelton] Calif State Polytech Univ Pomona, Dept Anim & Vet Sci, Pomona, CA 91768 USA. [Reddy, G. B.] N Carolina Agr & Tech State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA. RP Ibekwe, AM (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Mark.Ibekwe@ars.usda.gov RI Ma, Jincai/D-1290-2013 OI Ma, Jincai/0000-0002-0792-0251 FU 214 Manure and Byproduct Utilization Project of the USDA-ARS FX This research was supported by the 214 Manure and Byproduct Utilization Project of the USDA-ARS. We thank Damon Baptista for the technical assistance. Mention of trademark or proprietary products in this manuscript does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 38 TC 3 Z9 3 U1 9 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 15 PY 2016 VL 544 BP 68 EP 76 DI 10.1016/j.scitotenv.2015.11.139 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC8TB UT WOS:000369491500009 PM 26657250 ER PT J AU Zhou, DC Zhang, LX Hao, L Sun, G Liu, YQ Zhu, C AF Zhou, Decheng Zhang, Liangxia Hao, Lu Sun, Ge Liu, Yongqiang Zhu, Chao TI Spatiotemporal trends of urban heat island effect along the urban development intensity gradient in China SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Urbanization; Urban heat island; Spatiotemporal variations; Background climate; Land use activities; MODIS ID 32 MAJOR CITIES; NEW-YORK-CITY; LAND-SURFACE; AIR-POLLUTION; TIME-SERIES; 2 DECADES; URBANIZATION; CLIMATE; CLASSIFICATION; BIODIVERSITY AB Urban heat island (UHI) represents a major anthropogenic modification to the Earth system and its relationship with urban development is poorly understood at a regional scale. Using Aqua MODIS data and Landsat TM/ETM + images, we examined the spatiotemporal trends of the UHI effect (Delta T, relative to the rural reference) along the urban development intensity (UDI) gradient in 32 major Chinese cities from 2003 to 2012. We found that the daytime and nighttime Delta T increased significantly (p < 0.05, mostly in linear form) along a rising UDI for 27 and 30 out of 32 cities, respectively. More rapid increases were observed in the southeastern and northwestern parts of China in the day and night, respectively. Moreover, the Delta T trends differed greatly by season and during daytime in particular. The Delta T increased more rapidly in summer than in winter during the day and the reverse occurred at night for most cities. Inter-annually, the Delta T increased significantly in about one-third of the cities during both the day and night times from 2003 to 2012, especially in suburban areas (0.25 < UDI <= 0.5), with insignificant trends being observed for most of the remaining cities. We also found that the Delta T patterns along the UDI gradient were largely controlled by local climate-vegetation conditions, while that across years were dominated by human activities. Our results highlight the strong and highly diverse urbanization effects on local climate cross China and offer limitations on how these certain methods should be used to quantify UHI intensity over large areas. Furthermore, the impacts of urbanization on climate are complex, thus future research efforts should focus more toward direct observation and physical-based modeling to make credible predictions of the effects. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhou, Decheng; Zhang, Liangxia; Hao, Lu] Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Jiangsu Key Lab Agr Meteorol, Nanjing 210044, Peoples R China. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, USDA, Athens, GA 30602 USA. [Zhu, Chao] Minist Environm Protect PR China, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China. RP Zhou, DC (reprint author), 219,Ningliu Rd, Nanjing 210044, Jiangsu, Peoples R China. EM zhoudc@nuist.edu.cn FU National Natural Science Foundation of China [41501465]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [15KJB170013]; Startup Foundation for Introducing Talent of NUIST [2014r051] FX This study was supported by the National Natural Science Foundation of China (#41501465), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (#15KJB170013), and the Startup Foundation for Introducing Talent of NUIST (2014r051). We thank Dr. Johnny Boggs (Eastern Forest Environmental Threat Assessment Center, USDA Forest Service Southern Research Station) and three anonymous reviewers for their valuable comments to improve this manuscript. NR 46 TC 4 Z9 4 U1 11 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 15 PY 2016 VL 544 BP 617 EP 626 DI 10.1016/j.scitotenv.2015.11.168 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DC8TB UT WOS:000369491500067 PM 26674691 ER PT J AU Magney, TS Eitel, JUH Huggins, DR Vierling, LA AF Magney, Troy S. Eitel, Jan U. H. Huggins, David R. Vierling, Lee A. TI Proximal NDVI derived phenology improves in-season predictions of wheat quantity and quality SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Normalized Difference Vegetation Index; Phenology; Proximal remote sensing; Agronomy; Grain quality; Yield prediction; Phenotyping; Spectral reflectance sensors ID SPECTRAL REFLECTANCE MEASUREMENTS; LOCALLY WEIGHTED REGRESSION; ADJUSTED VEGETATION INDEX; DRY-MATTER PRODUCTION; WINTER-WHEAT; GRAIN-YIELD; CANOPY REFLECTANCE; SPRING WHEAT; STAY-GREEN; NITROGEN STATUS AB Automated, low-cost and field-deployable remote sensing tools are well suited for continuously monitoring crop growth and providing growers with timely information about crop performance. Because automated sensors provide information about crop development and performance across time, we examined the hypothesis that ground-based canopy reflectance data might define crop phenology in new ways over the course of the season that can better forecast crop yield, protein, biomass, and grain nitrogen at harvest. This study examines the utility of daily Normalized Difference Vegetation Index (NDVI) data to monitor crop phenology over two complete growing seasons. Spectral reflectance data was collected at a total of sixteen plots under four different applied nitrogen (N) and soil water availability scenarios in rainfed soft white spring wheat (Triticum aestivum L.). Using NDVI at solar noon, four phenological periods were derived from the data using a non-parametric regression locally weighted smoothing parameter (loess) to account for day to day variability, and piecewise linear regression to determine inflection points in the seasonal NDVI curve. The NDVI derived phenological metrics (i.e. the change in NDVI per day, and duration (in days) of each phenological period) were compared against daily NDVI values throughout the season to predict harvest metrics. Daily NDVI data were generally poor predictors of harvest metrics early in the growing season, and reached maximum predictive power at the onset of heading, and the middle of ripening for biomass and yield (R-2 similar to 0.50 and similar to 0.25 during heading, respectively, and R-2 similar to 0.50 during early ripening). Conversely, using both simple and multiple regression analysis, we found that harvest metrics were better explained using the rate and duration of NDVI derived phenological periods. Simple regressions between NDVI derived phenological metrics revealed several physiologically and management relevant correlations including strong, statistically significant (p < 0.05) relationships between the rate of tillering and stem extension and total biomass (R-2 = 0.63 and 0.54, respectively), the duration of heading and yield (R-2 = 0.67), the rate of ripening and grain protein concentration (R-2 = 0.45), and the duration of ripening and grain N content (R-2 = 0.43), for example. Using multiple regression analysis, 83% of the variance in yield, 67% in protein concentration, 87% in total biomass, and 80% in grain N was explained by two to three NDVI derived phenological metrics. Further, multiple regression analysis using NDVI derived phenological metrics from the early season (tillering and stem extension) substantially improved early prediction of yield and biomass as compared to daily NDVI data, whereas protein and grain N were primarily driven by metrics associated with the reproductive development of the crop (heading and ripening). This work has implications for improving in-season management decisions and understanding of the phenological drivers of harvest metrics using daily NDVI data as an evaluation tool. (C) 2015 Elsevier B.V. All rights reserved. C1 [Magney, Troy S.; Eitel, Jan U. H.; Vierling, Lee A.] Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA. [Eitel, Jan U. H.; Vierling, Lee A.] Univ Idaho, McCall Outdoor Sci Sch, Mccall, ID 83638 USA. [Huggins, David R.] ARS, USDA, Pullman, WA 99163 USA. [Magney, Troy S.] NASA, Jet Prop Lab, MS 233-300,4800 Oak Grove Dr, Pasadena, CA 91109 USA. RP Magney, TS (reprint author), Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA.; Magney, TS (reprint author), NASA, Jet Prop Lab, MS 233-300,4800 Oak Grove Dr, Pasadena, CA 91109 USA. EM tmagney@uidaho.edu; jeitel@uidaho.edu; dhuggins@wsu.edu; leev@uidaho.edu FU US Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) award [2011 - 637003-3034]; NASA Idaho Space Grant Fellowship [NNX10AM75H] FX Many thanks to Jyoti Jennowoin, Dave Uberaga, Sam Finch, Leanna Dann for experimental design and field support, and Drs. Steven R. Garrity and Kevin L. Griffin for comments during previous versions and developments of this work. This research was made possible through funding provided by US Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) award 2011 - 637003-3034 and the NASA Idaho Space Grant Fellowship awarded to TSM (#NNX10AM75H). NR 93 TC 1 Z9 1 U1 15 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD FEB 15 PY 2016 VL 217 BP 46 EP 60 DI 10.1016/j.agrformet.2015.11.009 PG 15 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DB9VL UT WOS:000368865700005 ER PT J AU White, KE Reeves, JB Coale, FJ AF White, Kathryn E. Reeves, James B., III Coale, Frank J. TI Cell wall compositional changes during incubation of plant roots measured by mid-infrared diffuse reflectance spectroscopy and fiber analysis SO GEODERMA LA English DT Article DE Infrared spectroscopy; Roots; Decomposition; Lignin; Cellulose ID SOIL ORGANIC-MATTER; CARBOHYDRATE-COMPOSITION; LIGNIN COMPOSITION; FTIR SPECTROSCOPY; LITTER CHEMISTRY; CARBON; DECOMPOSITION; RESIDUES; DYNAMICS; MINERALIZATION AB Plant roots, particularly the constituents of root cell walls (hemicellulose, cellulose and lignin) are important contributors to soil organic matter. Little is known about the cell wall composition of many important crop species or compositional changes as roots decay. The objectives of this study were to quantify changes in root cell wall composition during a four week laboratory incubation by forage fiber analysis and characterize those changes using diffuse reflectance infrared fourier transform spectroscopy (DRIFTS). The roots of six important crop, forage and native grass species were incubated at 25 degrees C and sampled weekly. Alfalfa lost 78% of initial mass over four weeks, while the remaining species lost between 19% and 38%. For all species the majority of this loss occurred during Week 1, and only alfalfa mass loss was significant (P < 0.05) each week. The trends observed for whole root decomposition were paralleled by the decomposability of root cell walls. Significant changes in hemicellulose, cellulose and lignin concentrations over time were only observed in alfalfa roots. Significant changes in decomposability of these constituents was likewise only observed in alfalfa, with cellulose the most decomposable fraction, followed by hemicellulose and lignin. Analysis by DRIFTS supported the fiber analysis results and revealed important changes in root cell wall composition. The disappearance of peaks due to starch in the perennial alfalfa and switchgrass roots following Week 1 helped to explain the greater initial mass loss in both of these species relative to the roots of the annuals. The spectral data also illustrated the resistance of alfalfa lignin to decomposition, the preservation of carbonyl compounds and the degradation of readily decomposed proteins. Finally, changes potentially indicative of wax compound preservation were found in the DRIFTS spectra of alfalfa even though the amount of wax was too small to quantify by fiber analysis. This research study reveals differences in the rate at which crop roots decompose and important changes that can occur in readily decomposable roots over relatively short time scales. These results provide valuable information contributing to the understanding and prediction of short term soil organic matter dynamics which will help to predict possible impact of management changes or soil disturbance on soil health and productivity as well as long term organic C stabilization and the potential for C sequestration. Published by Elsevier B.V. C1 [White, Kathryn E.; Reeves, James B., III] ARS, USDA, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. [White, Kathryn E.; Coale, Frank J.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. RP White, KE (reprint author), ARS, USDA, Sustainable Agr Syst Lab, 10300 Baltimore Ave,Bldg 001 BARC West, Beltsville, MD 20705 USA. EM Kate.White@ars.usda.gov NR 54 TC 0 Z9 0 U1 7 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD FEB 15 PY 2016 VL 264 BP 205 EP 213 DI 10.1016/j.geoderma.2015.10.018 PG 9 WC Soil Science SC Agriculture GA CZ0DU UT WOS:000366776100022 ER PT J AU Charrondiere, UR Rittenschober, D Nowak, V Stadlmayr, B Wijesinha-Bettoni, R Haytowitz, D AF Charrondiere, U. Ruth Rittenschober, Doris Nowak, Verena Stadlmayr, Barbara Wijesinha-Bettoni, Ramani Haytowitz, David TI Improving food composition data quality: Three new FAO/INFOODS guidelines on conversions, data evaluation and food matching SO FOOD CHEMISTRY LA English DT Article DE Food composition; FAO/INFOODS; Guidelines; Food matching; Conversion; Checking; Data quality AB Food composition data play a key role in many sectors and the availability of quality data is critically important. Since 1984, the International Network of Food Data Systems (INFOODS) has been working towards improving food composition data quality and availability, including the development and updating of standards, guidelines and tools for food composition. FAO/INFOODS has recently published three comprehensive guidelines to improve and harmonise the compilation of data: (1) Guidelines for Food Matching, (2) Guidelines for Checking Food Composition Data prior to Publication of a User Table/Database, and (3) Guidelines for Converting Units, Denominators and Expressions. This article describes their content and development processes. Their adoption, along with additional ones planned for the future by FAO/INFOODS, should further improve the quality of published food composition data, which in turn can lead to more accurate nutrient intake estimates and more precise food labels, as well as better-targeted programs and policies. (c) 2015 Food and Agriculture Organization of the United Nations (FAO). Published by Elsevier Ltd. All rights reserved. C1 [Charrondiere, U. Ruth] FAO, I-00153 Rome, Italy. USDA, Nutrient Data Lab, Beltsville, MD 20705 USA. RP Charrondiere, UR (reprint author), FAO, Viale Terme Caracalla, I-00153 Rome, Italy. OI Nowak, Verena/0000-0003-2523-579X NR 37 TC 0 Z9 0 U1 0 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD FEB 15 PY 2016 VL 193 BP 75 EP 81 DI 10.1016/j.foodchem.2014.11.055 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CT7EW UT WOS:000362977800013 PM 26433290 ER PT J AU Dickey, AM Loy, JD Bono, JL Smith, TPL Apley, MD Lubbers, BV DeDonder, KD Capik, SF Larson, RL White, BJ Blom, J Chitko-McKown, CG Clawson, ML AF Dickey, Aaron M. Loy, John D. Bono, James L. Smith, Timothy P. L. Apley, Mike D. Lubbers, Brian V. DeDonder, Keith D. Capik, Sarah F. Larson, Robert L. White, Brad J. Blom, Jochen Chitko-McKown, Carol G. Clawson, Michael L. TI Large genomic differences between Moraxella bovoculi isolates acquired from the eyes of cattle with infectious bovine keratoconjunctivitis versus the deep nasopharynx of asymptomatic cattle SO VETERINARY RESEARCH LA English DT Article ID SP-NOV; STRAINS; CALVES; VACCINE; IDENTIFICATION; PHYLOGENIES; NETWORKS; TREES; SPP.; TOOL AB Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or "pinkeye" in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from the eyes of IBK cases profoundly differed from those that originated from the nasopharynx of asymptomatic cattle in genome structure, gene content and polymorphism diversity and consequently placed into two distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. bovoculi that may not associate with IBK. C1 [Dickey, Aaron M.; Bono, James L.; Smith, Timothy P. L.; Chitko-McKown, Carol G.; Clawson, Michael L.] ARS, USDA, USMARC, State Spur 18D, Clay Ctr, NE 68933 USA. [Loy, John D.] Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE 68583 USA. [Apley, Mike D.; DeDonder, Keith D.; Capik, Sarah F.; Larson, Robert L.; White, Brad J.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. [Lubbers, Brian V.] Kansas State Univ, Kansas State Diagnost Lab, Manhattan, KS 66506 USA. [Blom, Jochen] Univ Giessen, Bioinformat & Syst Biol, D-35392 Giessen, Germany. RP Clawson, ML (reprint author), ARS, USDA, USMARC, State Spur 18D, Clay Ctr, NE 68933 USA. EM mike.clawson@ars.usda.gov OI Clawson, Michael/0000-0002-3355-5390 FU USDA National Institute of Food and Agriculture Capacity Animal Health [1007070, 1002196]; Agricultural Research Service FX This work was supported, in part, by the USDA National Institute of Food and Agriculture Capacity Animal Health projects 1007070 and 1002196 for testing and archival of bacterial isolates. The remainder of financial support was provided by the Agricultural Research Service. The authors thank Gennie Schuller, Joshua Payne and Miranda Timmons for outstanding technical support and Jan Watts for secretarial support. The use of product and company names is necessary to accurately report the methods and results; however, the United States Department of Agriculture (USDA) neither guarantees nor warrants the standard of the products, and the use of names by the USDA implies no approval of the product to the exclusion of others that may also be suitable. The USDA is an equal opportunity provider and employer. NR 55 TC 1 Z9 1 U1 2 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD FEB 13 PY 2016 VL 47 AR 31 DI 10.1186/s13567-016-0316-2 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA DD5DE UT WOS:000369942000001 PM 26872821 ER PT J AU Diep, CS Lemaitre, RN Chen, TA Baranowski, T Lutsey, PL Manichaikul, AW Rich, SS St-Jules, DE Steffen, BT Tsai, MY Siscovick, DS Frazier-Wood, AC AF Diep, Cassandra S. Lemaitre, Rozenn N. Chen, Tzu-An Baranowski, Tom Lutsey, Pamela L. Manichaikul, Ani W. Rich, Stephen S. St-Jules, David E. Steffen, Brian T. Tsai, Michael Y. Siscovick, David S. Frazier-Wood, Alexis C. TI Acculturation and Plasma Fatty Acid Concentrations in Hispanic and Chinese-American Adults: The Multi-Ethnic Study of Atherosclerosis SO PLOS ONE LA English DT Article ID CARDIOVASCULAR-DISEASE; UNITED-STATES; SOCIOECONOMIC-STATUS; HEART-ASSOCIATION; ASIAN-AMERICANS; RISK-FACTORS; HEALTH; IMMIGRANTS; BEHAVIORS; DIETARY AB Background Acculturation to the U.S. is associated with increased risk of cardiovascular disease, but the etiologic pathways are not fully understood. Plasma fatty acid levels exhibit ethnic differences and are emerging as biomarkers and predictors of cardiovascular disease risk. Thus, plasma fatty acids may represent one pathway underlying the association between acculturation and cardiovascular disease. We investigated the cross-sectional relationship between acculturation and plasma phospholipid fatty acids in a diverse sample of Hispanic- and Chinese-American adults. Methods and Findings Participants included 377 Mexican, 320 non-Mexican Hispanic, and 712 Chinese adults from the Multi-Ethnic Study of Atherosclerosis, who had full plasma phospholipid assays and acculturation information. Acculturation was determined from three proxy measures: nativity, language spoken at home, and years in the U.S., with possible scores ranging from 0 (least acculturated) to 5 (most acculturated) points. a-Linolenic acid, linoleic acid, eicosapentaenoic acid, docosahexaenoic acid, and arachidonic acid were measured in fasting plasma. Linear regression models were conducted in race/ethnicity-stratified analyses, with acculturation as the predictor and plasma phospholipid fatty acids as the outcome variables. We ran secondary analyses to examine associations between acculturation and dietary fatty acids for comparison. Covariates included age, gender, education, and income. Contrary to our hypothesis, no statistically significant associations were detected between acculturation and plasma phospholipid fatty acids for Chinese, non-Mexican Hispanic, or Mexican participants. However, acculturation was related to dietary total n-6 fatty acids and dietary n-3/n-6 ratios in expected directions for Mexican, non-Mexican Hispanic, and combined Hispanic participants. In Chinese individuals, acculturation was unexpectedly associated with lower arachidonic acid intake. Conclusion Absence of associations between acculturation and plasma phospholipid fatty acids suggests that changes in the plasma phospholipid fatty acids studied do not account for the observed associations of acculturation to the U.S. and cardiovascular disease risk. Similar findings were observed for eicosapentaenoic acid and docosahexaenoic acid, when using dietary intake. However, the observed associations between dietary n-6 fatty acids and acculturation in Hispanic individuals suggest that dietary intake may be more informative than phospholipids when investigating acculturation effects. In Chinese individuals, acculturation may have a possible protective effect through decreased arachidonic acid intake. Further research on dietary fatty acids and other cardiovascular disease biomarkers is needed to identify possible etiologic mechanisms between acculturation and cardiovascular disease. C1 [Diep, Cassandra S.; Chen, Tzu-An; Baranowski, Tom; Frazier-Wood, Alexis C.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Diep, Cassandra S.] Univ Texas MD Anderson Canc Ctr, Dept Hlth Dispar Res, Houston, TX 77030 USA. [Lemaitre, Rozenn N.] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA USA. [Lutsey, Pamela L.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Manichaikul, Ani W.; Rich, Stephen S.] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA USA. [St-Jules, David E.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Steffen, Brian T.; Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Siscovick, David S.] New York Acad Med, New York, NY USA. RP Diep, CS (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA.; Diep, CS (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Hlth Dispar Res, Houston, TX 77030 USA. EM cassandra.diep@gmail.com OI Manichaikul, Ani/0000-0002-5998-795X; Baranowski, Tom/0000-0002-0653-2222 FU USDA/ARS [58-6250-0-008]; National Research Service Award [T32 HP10031]; American Heart Association [14BGIA18740011]; National Heart, Lung, and Blood Institute (NHLBI); National Heart, Lung, and Blood Institute [N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC95162, N01-HC-95163, N01-HC-95164, N01-HC95165, N01-HC-95166, N01-HC-95167, N01-HC95168, N01-HC-95169]; NCRR [UL1-TR000040, UL1-TR-001079] FX This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and had been funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. CSD was supported by a Primary Care Research Training Grant from National Research Service Award (#T32 HP10031). ACF-W was supported in part by American Heart Association grant number 14BGIA18740011. MESA and the MESA SHARe project are conducted and supported by the National Heart, Lung, and Blood Institute (NHLBI) in collaboration with MESA investigators. Support for MESA is provided by contracts N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC95162, N01-HC-95163, N01-HC-95164, N01-HC95165, N01-HC-95166, N01-HC-95167, N01-HC95168, and N01-HC-95169 from the National Heart, Lung, and Blood Institute and by grants UL1-TR000040 and UL1-TR-001079 from NCRR. A full list of participating MESA investigators and institutions can be found at http://www.mesa-nhlbi.org. NR 49 TC 0 Z9 0 U1 1 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 12 PY 2016 VL 11 IS 2 AR e0149267 DI 10.1371/journal.pone.0149267 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DD6SI UT WOS:000370054100138 PM 26872329 ER PT J AU Fonseca-Garcia, C Coleman-Derr, D Garrido, E Visel, A Tringe, SG Partida-Martinez, LP AF Fonseca-Garcia, Citlali Coleman-Derr, Devin Garrido, Etzel Visel, Axel Tringe, Susannah G. Partida-Martinez, Laila P. TI The Cacti Microbiome: Interplay between Habitat-Filtering and Host-Specificity SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE arid and semi-arid ecosystems; Cactaceae; CAM plants; holobiont; Illumina amplicon sequencing; microbial diversity; microbiomes; plant-microbe interactions ID BACTERIAL COMMUNITY STRUCTURE; WEATHERING DESERT PLANTS; ENDOPHYTIC BACTERIA; RHIZOSPHERE MICROBIOME; FUNGAL ENDOPHYTES; GROWTH PROMOTION; ROOT MICROBIOTA; DIVERSITY; SOIL; PHYLLOSPHERE AB Cactaceae represents one of the most species-rich families of succulent plants native to arid and semi-arid ecosystems, yet the associations Cacti establish with microorganisms and the rules governing microbial community assembly remain poorly understood. We analyzed the composition, diversity, and factors influencing above- and below-ground bacterial, archaeal, and fungal communities associated with two native and sympatric Cacti species: Myrtillocactus geometrizans and Opuritia robusta. Phylogenetic profiling showed that the composition and assembly of microbial communities associated with Cacti were primarily influenced by the plant compartment; plant species, site, and season played only a minor role. Remarkably, bacterial, and archaeal diversity was higher in the phyllosphere than in the rhizosphere of Cacti, while the opposite was true for fungi. Semi-arid soils exhibited the highest levels of microbial diversity whereas the stem endosphere the lowest. Despite their taxonomic distance, M. geometrizans and O. robusta shared most microbial taxa in all analyzed compartments. Influence of the plant host did only play a larger role in the fungal communities of the stem endosphere. These results suggest that fungi establish specific interactions with their host plant inside the stem, whereas microbial communities in the other plant compartments may play similar functional roles in these two species. Biochemical and molecular characterization of seed-borne bacteria of Cacti supports the idea that these microbial symbionts may be vertically inherited and could promote plant growth and drought tolerance for the fitness of the Cacti holobiont. We envision this knowledge will help improve and sustain agriculture in arid and semi-arid regions of the world. C1 [Fonseca-Garcia, Citlali; Garrido, Etzel; Partida-Martinez, Laila P.] Ctr Invest & Estudios Avanzados, Dept Ingn Genet, Irapuato, Mexico. [Coleman-Derr, Devin; Visel, Axel; Tringe, Susannah G.] Joint Genome Inst, Dept Energy, Walnut Creek, CA USA. [Coleman-Derr, Devin; Visel, Axel; Tringe, Susannah G.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA. [Coleman-Derr, Devin] ARS, Ctr Plant Gene Express, USDA, Albany, CA USA. [Visel, Axel; Tringe, Susannah G.] Univ Calif Merced, Sch Nat Sci, Mol Cell Biol, Merced, CA USA. [Garrido, Etzel] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. RP Partida-Martinez, LP (reprint author), Ctr Invest & Estudios Avanzados, Dept Ingn Genet, Irapuato, Mexico. EM laila.partida@ira.cinvestav.mx RI Partida-Martinez, Laila/A-5935-2009; Visel, Axel/A-9398-2009 OI Partida-Martinez, Laila/0000-0001-8037-2856; Visel, Axel/0000-0002-4130-7784 FU Consejo Nacional de Ciencia y Tecnologia in Mexico (CONACyT) [CB-2010-01-151007, INFR-2012-01-197799]; JGI Community Science Program (CSP) [DE-AC02-05CH11231] FX LPP-M acknowledges Consejo Nacional de Ciencia y Tecnologia in Mexico (CONACyT), which supported this project with two grants: CB-2010-01-151007 and INFR-2012-01-197799 as well as the support from the JGI Community Science Program (CSP) under Contract No. DE-AC02-05CH11231. NR 83 TC 4 Z9 4 U1 19 U2 92 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 12 PY 2016 VL 7 AR 150 DI 10.3389/fmicb.2016.00150 PG 16 WC Microbiology SC Microbiology GA DD5EX UT WOS:000369946600001 PM 26904020 ER PT J AU Pettis, JS Rice, N Joselow, K vanEngelsdorp, D Chaimanee, V AF Pettis, Jeffery S. Rice, Nathan Joselow, Katie vanEngelsdorp, Dennis Chaimanee, Veeranan TI Colony Failure Linked to Low Sperm Viability in Honey Bee (Apis mellifera) Queens and an Exploration of Potential Causative Factors SO PLOS ONE LA English DT Article ID UNITED-STATES; NOSEMA-CERANAE; HYMENOPTERA; APIDAE; COUMAPHOS; LOSSES; HEALTH; SUPERSEDURE; FLUVALINATE; OPERATIONS AB Queen health is closely linked to colony performance in honey bees as a single queen is normally responsible for all egg laying and brood production within the colony. In the U.S. in recent years, queens have been failing at a high rate; with 50% or greater of queens replaced in colonies within 6 months when historically a queen might live one to two years. This high rate of queen failure coincides with the high mortality rates of colonies in the US, some years with >50% of colonies dying. In the current study, surveys of sperm viability in US queens were made to determine if sperm viability plays a role in queen or colony failure. Wide variation was observed in sperm viability from four sets of queens removed from colonies that beekeepers rated as in good health (n = 12; average viability = 92%), were replacing as part of normal management (n = 28; 57%), or where rated as failing (n = 18 and 19; 54% and 55%). Two additional paired set of queens showed a statistically significant difference in viability between colonies rated by the beekeeper as failing or in good health from the same apiaries. Queens removed from colonies rated in good health averaged high viability (ca. 85%) while those rated as failing or in poor health had significantly lower viability (ca. 50%). Thus low sperm viability was indicative of, or linked to, colony performance. To explore the source of low sperm viability, six commercial queen breeders were surveyed and wide variation in viability (range 60-90%) was documented between breeders. This variability could originate from the drones the queens mate with or temperature extremes that queens are exposed to during shipment. The role of shipping temperature as a possible explanation for low sperm viability was explored. We documented that during shipment queens are exposed to temperature spikes (<8 and > 40 degrees C) and these spikes can kill 50% or more of the sperm stored in queen spermathecae in live queens. Clearly low sperm viability is linked to colony performance and laboratory and field data provide evidence that temperature extremes are a potential causative factor. C1 [Pettis, Jeffery S.; Rice, Nathan; Joselow, Katie] USDA ARS, Bee Res Lab, Beltsville, MD USA. [vanEngelsdorp, Dennis] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Chaimanee, Veeranan] Maejo Univ, Dept Biotechnol, Phrae Campus, Rong Kwang, Phrae, Thailand. RP Chaimanee, V (reprint author), Maejo Univ, Dept Biotechnol, Phrae Campus, Rong Kwang, Phrae, Thailand. EM chveeranan@gmail.com FU USDA-ARS Areawide Program funds FX USDA-ARS Areawide Program funds supported this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 4 Z9 4 U1 11 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 10 PY 2016 VL 11 IS 2 AR e0147220 DI 10.1371/journal.pone.0147220 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DD6PR UT WOS:000370046600022 PM 26863438 ER PT J AU Bilyeu, KD Wiebold, WJ AF Bilyeu, Kristin D. Wiebold, William J. TI Environmental Stability of Seed Carbohydrate Profiles in Soybeans Containing Different Alleles of the Raffinose Synthase 2 (RS2) Gene SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soybean; [Glycine max (L.) Merr]; sucrose; stachyose; carbohydrate; environment ID FAMILY OLIGOSACCHARIDES; GALACTOSYL CYCLITOLS; STACHYOSE; TOLERANCE; PHENOTYPE; TRAITS AB Soybean [Glycine max (L.) Merr.] is important for the high protein meal used for livestock feed formulations. Carbohydrates contribute positively or negatively to the potential metabolizable energy in soybean meal. The positive carbohydrate present in soybean meal consists primarily of sucrose, whereas the negative carbohydrate components are the raffinose family of oligosaccharides (RFOs), raffinose and stachyose. Increasing sucrose and decreasing raffinose and stachyose are critical targets to improve soybean. In three recently characterized lines, variant alleles of the soybean raffinose synthase 2 (RS2) gene were associated with increased sucrose and decreased RFOs. The objective of this research was to compare the environmental stability of seed carbohydrates in soybean lines containing wild-type or variant alleles of RS2 utilizing a field location study and a date of planting study. The results define the carbohydrate variation in distinct regional and temporal environments using soybean lines with different alleles Of the RS2 gene. C1 [Bilyeu, Kristin D.] Univ Missouri, USDA ARS, Plant Genet Res Unit, 110 Waters Hall, Columbia, MO 65211 USA. [Wiebold, William J.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Bilyeu, KD (reprint author), Univ Missouri, USDA ARS, Plant Genet Res Unit, 110 Waters Hall, Columbia, MO 65211 USA. EM Kristin.Bilyeu@ars.usda.gov FU United Soybean Board FX This research was supported by a grant to K.D.B. from the United Soybean Board. NR 25 TC 0 Z9 0 U1 2 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 10 PY 2016 VL 64 IS 5 BP 1071 EP 1078 DI 10.1021/acs.jafc.5b04779 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DD8ZO UT WOS:000370215600004 PM 26800264 ER PT J AU Hacisalihoglu, G Gustin, JL Louisma, J Armstrong, P Peter, GF Walker, AR Settles, AM AF Hacisalihoglu, Gokhan Gustin, Jeffery L. Louisma, Jean Armstrong, Paul Peter, Gary F. Walker, Alejandro R. Settles, A. Mark TI Enhanced Single Seed Trait Predictions in Soybean (Glycine max) and Robust Calibration Model Transfer with Near-Infrared Reflectance Spectroscopy SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE seed phenotyping; near-infrared spectroscopy; chemometrics; microcomputed tomography; density; oil; protein ID MULTIVARIATE INSTRUMENT STANDARDIZATION; MAIZE; ATTRIBUTES; SELECTION; SPECTRA; DENSITY; PROTEIN; WEIGHT; GRAIN; CORN AB Single seed near-infrared reflectance (NIR) spectroscopy predicts soybean (Glycine max) seed quality traits of moisture, oil, and protein. We tested the accuracy of transferring calibrations between different single seed NIR analyzers of the same design by collecting NIR spectra and analytical trait data for globally diverse soybean germplasm. X-ray microcomputed tomography (mu CT) was used to collect seed density and shape traits to enhance the number of soybean traits that can be predicted from single seed NIR Partial least-squares (PLS) regression gave accurate predictive models for oil, weight, volume, protein, and maximal cross-sectional area of the seed. PLS models for width, length, and density were not predictive. Although principal-component analysis (PCA) of the NIR spectra showed that black seed coat color had significant signal, excluding black seeds from the calibrations did not impact model accuracies. Calibrations for oil and protein developed in this study as well as earlier calibrations for a separate NIR analyzer of the same design were used to test the ability to transfer PLS regressions between platforms. PLS models built from data collected on one NIR analyzer had minimal differences in accuracy when applied to spectra collected from a sister device. Model transfer was more robust when spectra were trimmed from 910 to 1679 nm to 955-1635 run due to divergence of edge wavelengths between the two devices. The ability to transfer calibrations between similar single seed NIR spectrometers facilitates broader adoption of this high-throughput, nondestructive, seed phenotyping technology.: C1 [Hacisalihoglu, Gokhan; Louisma, Jean] Florida A&M Univ, Dept Biol, Tallahassee, FL 32307 USA. [Gustin, Jeffery L.; Settles, A. Mark] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Armstrong, Paul] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Peter, Gary F.; Walker, Alejandro R.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. RP Settles, AM (reprint author), Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. EM settles@ufl.edu OI Settles, A. Mark/0000-0002-5846-0996 FU National Science Foundation Plant Genome Research Program [IOS-1031416, IOS-1111156]; National Institute of Food and Agriculture [2011-67003-30215]; Vasil-Monsanto Endowment FX This work was supported by the National Science Foundation Plant Genome Research Program awards IOS-1031416 and IOS-1111156, National Institute of Food and Agriculture award 2011-67003-30215, and the Vasil-Monsanto Endowment. NR 29 TC 1 Z9 1 U1 6 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 10 PY 2016 VL 64 IS 5 BP 1079 EP 1086 DI 10.1021/acs.jafc.5b05508 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DD8ZO UT WOS:000370215600005 PM 26771201 ER PT J AU Nol, P Olsen, SC Rhyan, JC Sriranganathan, N McCollum, MP Hennager, SG Pavuk, AA Sprino, PJ Boyle, SM Berrier, RJ Salman, MD AF Nol, Pauline Olsen, Steven C. Rhyan, Jack C. Sriranganathan, Nammalwar McCollum, Matthew P. Hennager, Steven G. Pavuk, Alana A. Sprino, Phillip J. Boyle, Stephen M. Berrier, Randall J. Salman, Mo D. TI Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE Brucella abortus; RB51; elk; wildlife vaccination; superoxide dismutase; O-side chain; glycosyltransferase ID YERSINIA-ENTEROCOLITICA O-9; IMMUNE-RESPONSES; SEROLOGICAL REACTIONS; BOVINE BRUCELLOSIS; INFECTION; EFFICACY; CATTLE; LIPOPOLYSACCHARIDE; BISON; VIRULENCE AB In recent years, elk (Cervus canadensis) have been implicated as the source of Brucella abortus infection for numerous cattle herds in the Greater Yellowstone Area. In the face of environmental and ecological changes on the landscape, the range of infected elk is expanding. Consequently, the development of effective disease management strategies for wild elk herds is of utmost importance, not only for the prevention of reintroduction of brucellosis to cattle, but also for the overall health of the Greater Yellowstone Area elk populations. In two studies, we evaluated the efficacy of B. abortus strain RB51 over-expressing superoxide dismutase and glycosyltransferase for protecting elk from infection and disease caused by B. abortus after experimental infection with a virulent B. abortus strain. Our data indicate that the recombinant vaccine does not protect elk against brucellosis. Further, work is needed for development of an effective brucellosis vaccine for use in elk. C1 [Nol, Pauline; Rhyan, Jack C.; McCollum, Matthew P.] US Anim & Plant Hlth Inspect Serv, Wildlife Livestock Dis Invest Team, Vet Serv, USDA, Ft Collins, CO USA. [Olsen, Steven C.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. [Sriranganathan, Nammalwar; Boyle, Stephen M.] Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA. [Hennager, Steven G.] US Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, Vet Serv, USDA, Ames, IA USA. [Pavuk, Alana A.] Colorado State Univ, Coll Vet Med & Biol Sci, Dept Pathobiol & Microbiol, Ft Collins, CO 80523 USA. [Sprino, Phillip J.; Berrier, Randall J.] Colorado Serum Co, Denver, CO USA. [Salman, Mo D.] Colorado State Univ, Dept Clin Sci, Coll Vet Med & Biol Sci, Ft Collins, CO 80523 USA. [Pavuk, Alana A.] Antech Diagnost, Chapel Hill, NC USA. RP Nol, P (reprint author), US Anim & Plant Hlth Inspect Serv, Wildlife Livestock Dis Invest Team, Vet Serv, USDA, Ft Collins, CO USA. EM pauline.nol@aphis.usda.gov FU United States Department of Agriculture FX This research was funded by the United States Department of Agriculture. NR 31 TC 0 Z9 0 U1 5 U2 9 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD FEB 10 PY 2016 VL 6 AR 10 DI 10.3389/fcimb.2016.00010 PG 10 WC Immunology; Microbiology SC Immunology; Microbiology GA DD1LZ UT WOS:000369684500001 PM 26904509 ER PT J AU Peng, J Walsh, PJ Sabo, RC Turng, LS Clemons, CM AF Peng, Jun Walsh, Philip J. Sabo, Ronald C. Turng, Lih-Sheng Clemons, Craig M. TI Water-assisted compounding of cellulose nanocrystals into polyamide 6 for use as a nucleating agent for microcellular foaming SO POLYMER LA English DT Article DE High-pressure water-assisted compounding; Microcellular injection molding; Cellulose nanocrystals; Polyamide 6; Crystal transformation ID INJECTION-MOLDED PARTS; MECHANICAL-PROPERTIES; NANOCOMPOSITES; EXTRUSION; CRYSTALLIZATION; POLYPROPYLENE; NYLONS; DISSOLUTION; PRESSURE; POLYMERS AB Cellulose nanocrystals (CNCs) are a biorenewable filler and can be an excellent nucleating agent for the development of microcellular foamed polymeric nanocomposites. However, their relatively low degradation temperature limits their use with engineering resins like polyamide 6 (PA6) in typical melt processing techniques such as injection molding, compounding, and extrusion. A water-assisted extrusion compounding process was investigated to directly compound CNC suspensions with PA6 without the need of predrying the CNCs. By using water as a plasticizer and reducing the processing temperature by 30 degrees C, this process can mitigate the degradation of CNCs during compounding. The effects of the CNCs on the mechanical properties, crystal type, and microstructure of solid and microcellular foamed specimens were characterized. The CNCs primarily acted as a nucleating filler, affecting both the matrix crystal structure and, in foamed composites, the cell structure. The CNCs nucleated the alpha-crystalline form of PA6 and also acted as a foam cell nucleator, increasing cell density by an order of magnitude while significantly reducing cell size. The weight reduction of the foamed specimens was about 15%. Adding small amounts of CNCs also increased matrix orientation in the solid injection molded specimens. These factors helped to improve the mechanical performance, especially the modulus of elasticity. During water-assisted compounding, thermal hydrolysis of PA6 occurred and generated carbonecarbon double bonds, as evaluated by FTIR. However, the molecular weight reduction caused by hydrolysis was less than 5%. The total molecular weight reduction was around 18%, combined with the melt extrusion and injection molding processes. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Peng, Jun; Turng, Lih-Sheng] Univ Wisconsin, Ctr Polymer Engn, Madison, WI 53706 USA. [Peng, Jun; Turng, Lih-Sheng] Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI 53715 USA. [Walsh, Philip J.; Sabo, Ronald C.; Clemons, Craig M.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Turng, LS (reprint author), Univ Wisconsin, Ctr Polymer Engn, Madison, WI 53706 USA.; Clemons, CM (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM turng@engr.wisc.edu; cclemons@fs.fed.us OI Peng, Jun/0000-0002-2337-5273; Turng, Lih-Sheng/0000-0001-8022-9224 FU USDA National Institute of Food and Agriculture Award [2011-67009-20056]; National Fundamental Research Funds for the Central Universities [20152M056] FX This project was supported by the USDA National Institute of Food and Agriculture Award (No. 2011-67009-20056). The authors would like to acknowledge Rick Reiner at the Forest Products Laboratory for preparing the CNCs. Debby Sherman at Purdue University is kindly acknowledged for TEM imaging. The first author also wishes to acknowledge the National Fundamental Research Funds for the Central Universities (No. 20152M056) for their financial support. NR 46 TC 4 Z9 4 U1 16 U2 65 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0032-3861 EI 1873-2291 J9 POLYMER JI Polymer PD FEB 10 PY 2016 VL 84 BP 158 EP 166 DI 10.1016/j.polymer.2015.12.050 PG 9 WC Polymer Science SC Polymer Science GA DC3VD UT WOS:000369146100019 ER PT J AU Falconer, JL Christie, RJ Pollard, EJ Olsen, SC Grainger, DW AF Falconer, Jonathan L. Christie, R. James Pollard, Emily J. Olsen, Steven C. Grainger, David W. TI Live RB51 vaccine lyophilized hydrogel formulations with increased shelf life for practical ballistic delivery SO INTERNATIONAL JOURNAL OF PHARMACEUTICS LA English DT Article DE Wildlife; Animal health; Brucellosis; Remote delivery; Bison; Polymerization; Bio-bullet; Polyethylene glycol ID ABORTUS STRAIN RB51; BISON BISON-BISON; YELLOWSTONE-NATIONAL-PARK; BRUCELLA-ABORTUS; IMMUNOLOGICAL RESPONSES; BACTERIAL SURVIVAL; SAFETY; CATTLE; POLYMERIZATION; PATHOLOGY AB Ballistic delivery capability is essential to delivering vaccines and other therapeutics effectively to both livestock and wildlife in many global scenarios. Here, lyophilized poly(ethylene glycol) (PEG)-glycolide dimethacrylate crosslinked but degradable hydrogels were assessed as payload vehicles to protect and deliver a viable bacterial vaccine, Brucella abortus strain RB51 (RB51), ballistically using commercial thermoplastic cellulosic degradable biobullets. Degradable PEG hydrogel rods loaded with similar to 10(10) live RB51 bacteria (CFUs) were fabricated using three different polymerization methods, cut into fixed-sized payload segments, and lyophilized. Resulting dense, glassy RB51 vaccine-loaded monoliths were inserted into thermoplastic biobullet 100-mL payload chambers. Viability studies of lyophilized formulations assessed as a function of time and storage temperature supported the abilities of several conditions to produce acceptable vaccine shelf-lives. Fired from specifically designed air rifles, gel-loaded biobullets exhibit down-range ballistic properties (i.e., kinetic energy, trajectory, accuracy) similar to unloaded biobullets. Delivered to bovine tissue, these hydrogels rehydrate rapidly by swelling in tissue fluids, with complete hydration observed after 5 h in serum. Live RB51 vaccine exhibited excellent viability following carrier polymerization, lyophilization, and storage, at levels sufficient for vaccine dosing to wild range bison, the intended target. These data validate lyophilized degradable PEG hydrogel rods as useful drug carriers for remote delivery of both live vaccines and other therapeutics to livestock, wildlife, or other free-range targets using ballistic technologies. (C) 2015 Elsevier B.V. All rights reserved. C1 [Falconer, Jonathan L.; Grainger, David W.] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA. [Christie, R. James; Pollard, Emily J.] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA. [Olsen, Steven C.] USDA ARS, Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA. RP Grainger, DW (reprint author), Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA. EM david.grainger@utah.edu FU National Park Service [1200-99-009, P12AC15063] FX Support from National Park Service contracts 1200-99-009 and P12AC15063 (to DWG) is acknowledged. The authors thank R. Wallen, Bison Ecology Lab, Yellowstone National Park, for initiating and supporting this collaborative project, R. Hansen, SolidTech Animal Health (USA), Colorado Serum Company (USA) for assistance with biobullet components and RB51 viability and formulation discussions, respectively. The authors also thank Benjamin Carney for competent technical contributions. NR 48 TC 0 Z9 0 U1 3 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-5173 EI 1873-3476 J9 INT J PHARMACEUT JI Int. J. Pharm. PD FEB 10 PY 2016 VL 498 IS 1-2 BP 187 EP 194 DI 10.1016/j.ijpharm.2015.12.040 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DB1SW UT WOS:000368290200019 PM 26705151 ER PT J AU Federhen, S Rossello-Mora, R Klenk, HP Tindall, BJ Konstantinidis, KT Whitman, WB Brown, D Labeda, D Ussery, D Garrity, GM Colwell, RR Hasan, N Graf, J Parte, A Yarza, P Goldberg, B Sichtig, H Karsch-Mizrachi, I Clark, K McVeigh, R Pruitt, KD Tatusova, T Falk, R Turner, S Madden, T Kitts, P Kimchi, A Klimke, W Agarwala, R DiCuccio, M Ostell, J AF Federhen, Scott Rossello-Mora, Ramon Klenk, Hans-Peter Tindall, Brian J. Konstantinidis, Konstantinos T. Whitman, William B. Brown, Daniel Labeda, David Ussery, David Garrity, George M. Colwell, Rita R. Hasan, Nur Graf, Joerg Parte, Aidan Yarza, Pablo Goldberg, Brittany Sichtig, Heike Karsch-Mizrachi, Ilene Clark, Karen McVeigh, Richard Pruitt, Kim D. Tatusova, Tatiana Falk, Robert Turner, Sean Madden, Thomas Kitts, Paul Kimchi, Avi Klimke, William Agarwala, Richa DiCuccio, Michael Ostell, James TI Meeting report: GenBank microbial genomic taxonomy workshop (12-13 May, 2015) SO STANDARDS IN GENOMIC SCIENCES LA English DT Article DE GenBank; Genomic taxonomy; Misidentified sequence entries ID SPECIES DEFINITION; PROKARYOTES; BACTERIA; ARCHAEA AB Many genomes are incorrectly identified at GenBank. We developed a plan to find and correct misidentified genomes using genomic comparison statistics together with a scaffold of reliably identified genomes from type. A workshop was organized with broad representation from the bacterial taxonomic community to review the proposal, the GenBank Microbial Genomic Taxonomy Workshop, Bethesda MD, May 12-13, 2015. C1 [Federhen, Scott; Karsch-Mizrachi, Ilene; Clark, Karen; McVeigh, Richard; Pruitt, Kim D.; Tatusova, Tatiana; Falk, Robert; Turner, Sean; Madden, Thomas; Kitts, Paul; Kimchi, Avi; Klimke, William; Agarwala, Richa; DiCuccio, Michael; Ostell, James] NCBI, Bethesda, MD USA. [Rossello-Mora, Ramon] IMEDEA CSIC UIB, Esporles, Spain. [Klenk, Hans-Peter] Newcastle Univ, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Tindall, Brian J.] Deutsch Sammlung Mikroorganism Zellkultur GmbH, Braunschweig, Germany. [Konstantinidis, Konstantinos T.] Georgia Inst Technol, Atlanta, GA 30332 USA. [Whitman, William B.] Univ Georgia, Athens, GA 30602 USA. [Brown, Daniel] Univ Florida, Gainesville, FL USA. [Labeda, David] USDA, Washington, DC 20250 USA. [Ussery, David] Oak Ridge Natl Lab, Oak Ridge, TN USA. [Garrity, George M.] Michigan State Univ, E Lansing, MI 48824 USA. [Colwell, Rita R.] Univ Maryland, College Pk, MD 20742 USA. [Hasan, Nur] CosmosID, Rockville, MD USA. [Graf, Joerg] Univ Connecticut, Storrs, CT USA. [Parte, Aidan] LPSN, New York, NY USA. [Yarza, Pablo] Ribocon, Bremen, Germany. [Goldberg, Brittany; Sichtig, Heike] US FDA, Silver Spring, MD USA. RP Federhen, S (reprint author), NCBI, Bethesda, MD USA. EM federhen@ncbi.nlm.nih.gov OI Parte, Aidan/0000-0002-6304-4217; Ussery, David/0000-0003-3632-5512; Graf, Joerg/0000-0001-5320-2712 NR 17 TC 7 Z9 7 U1 2 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1944-3277 J9 STAND GENOMIC SCI JI Stand. Genomic Sci. PD FEB 9 PY 2016 VL 11 AR 15 DI 10.1186/s40793-016-0134-1 PG 8 WC Genetics & Heredity; Microbiology SC Genetics & Heredity; Microbiology GA DO7FR UT WOS:000377948300002 ER PT J AU Morales, MG Denno, BD Miller, DR Miller, GL Ben-Dov, Y Hardy, NB AF Morales, Mayrolin Garcia Denno, Barbara D. Miller, Douglass R. Miller, Gary L. Ben-Dov, Yair Hardy, Nate B. TI ScaleNet: a literature-based model of scale insect biology and systematics SO DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION LA English DT Article ID GENETIC SYSTEMS; HEMIPTERA; COCCOIDEA; EVOLUTION AB Scale insects (Hemiptera: Coccoidea) are small herbivorous insects found on all continents except Antarctica. They are extremely invasive, and many species are serious agricultural pests. They are also emerging models for studies of the evolution of genetic systems, endosymbiosis and plant-insect interactions. ScaleNet was launched in 1995 to provide insect identifiers, pest managers, insect systematists, evolutionary biologists and ecologists efficient access to information about scale insect biological diversity. It provides comprehensive information on scale insects taken directly from the primary literature. Currently, it draws from 23 477 articles and describes the systematics and biology of 8194 valid species. For 20 years, ScaleNet ran on the same software platform. That platform is no longer viable. Here, we present a new, open-source implementation of ScaleNet. We have normalized the data model, begun the process of correcting invalid data, upgraded the user interface, and added online administrative tools. These improvements make ScaleNet easier to use and maintain and make the ScaleNet data more accurate and extendable. C1 [Morales, Mayrolin Garcia; Hardy, Nate B.] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Denno, Barbara D.; Miller, Douglass R.; Miller, Gary L.] ARS, USDA, Systemat Entomol Lab, Beltsville, MD 20705 USA. [Ben-Dov, Yair] Volcani Ctr, Agr Res Org, Bet Dagan, Israel. [Miller, Douglass R.] Div Plant Ind, Gainesville, FL 32608 USA. RP Morales, MG (reprint author), Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. EM mzg0043@tigermail.auburn.edu FU Auburn University; United States Department of Agriculture, Systematic Entomology Lab FX This work was supported by a Specific Cooperative Agreement between N.B.H. at Auburn University and G.L.M. at the United States Department of Agriculture, Systematic Entomology Lab. NR 11 TC 4 Z9 6 U1 3 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1758-0463 J9 DATABASE-OXFORD JI Database PD FEB 9 PY 2016 AR bav118 DI 10.1093/database/bav118 PG 5 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA DE9AK UT WOS:000370927600001 ER PT J AU Demmer, E Van Loan, MD Rivera, N Rogers, TS Gertz, ER German, JB Zivkovic, AM Smilowitz, JT AF Demmer, Elieke Van Loan, Marta D. Rivera, Nancy Rogers, Tara S. Gertz, Erik R. German, J. Bruce Zivkovic, Angela M. Smilowitz, Jennifer T. TI Consumption of a high-fat meal containing cheese compared with a vegan alternative lowers postprandial C-reactive protein in overweight and obese individuals with metabolic abnormalities: a randomised controlled cross-over study SO JOURNAL OF NUTRITIONAL SCIENCE LA English DT Article DE Dairy products; Inflammation; Metabolic syndrome; Obesity; Palm oil; Postprandial metabolism; Vegan diets ID TYPE-2 DIABETIC-PATIENTS; FACTOR-KAPPA-B; CARDIOVASCULAR-DISEASE; ENDOTHELIAL DYSFUNCTION; INFLAMMATORY MARKERS; VASCULAR-DISEASE; OXIDATIVE STRESS; MENSTRUAL-CYCLE; DAIRY-PRODUCTS; BLOOD-LIPIDS AB Dietary recommendations suggest decreased consumption of SFA to minimise CVD risk; however, not all foods rich in SFA are equivalent. To evaluate the effects of SFA in a dairy food matrix, as Cheddar cheese, v. SFA from a vegan-alternative test meal on postprandial inflammatory markers, a randomised controlled cross-over trial was conducted in twenty overweight or obese adults with metabolic abnormalities. Individuals consumed two isoenergetic highfat mixed meals separated by a 1-to 2-week washout period. Serum was collected at baseline, and at 1, 3 and 6 h postprandially and analysed for inflammatory markers (IL-6, IL-8, IL-10, IL-17, IL-18, TNF alpha, monocyte chemotactic protein-1 (MCP-1)), acute-phase proteins C-reactive protein (CRP) and serum amyloid-A (SAA), cellular adhesion molecules and blood lipids, glucose and insulin. Following both high-fat test meals, postprandial TAG concentrations rose steadily (P < 0 . 05) without a decrease by 6 h. The incremental AUC (iAUC) for CRP was significantly lower (P < 0.05) in response to the cheese compared with the vegan-alternative test meal. A treatment effect was not observed for any other inflammatory markers; however, for both test meals, multiple markers significantly changed from baseline over the 6 h postprandial period (IL-6, IL-8, IL-18, TNF alpha, MCP-1, SAA). Saturated fat in the form of a cheese matrix reduced the iAUC for CRP compared with a vegan-alternative test meal during the postprandial 6 h period. The study is registered at clinicaltrials.gov under NCT01803633. C1 [Demmer, Elieke; Van Loan, Marta D.; Rivera, Nancy; Rogers, Tara S.; Zivkovic, Angela M.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Van Loan, Marta D.; Gertz, Erik R.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [German, J. Bruce; Smilowitz, Jennifer T.] Univ Calif, Foods Hlth Inst, Davis, CA USA. [German, J. Bruce; Smilowitz, Jennifer T.] Univ Calif, Dept Food Sci & Technol, Davis, CA USA. RP Smilowitz, JT (reprint author), Univ Calif, Foods Hlth Inst, Davis, CA USA.; Smilowitz, JT (reprint author), Univ Calif, Dept Food Sci & Technol, Davis, CA USA. EM jensm@ucdavis.edu NR 66 TC 1 Z9 1 U1 7 U2 22 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 2048-6790 J9 J NUTR SCI JI J. Nutr. Sci. PD FEB 9 PY 2016 VL 5 AR UNSP e9 DI 10.1017/jns.2015.40 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DE4YP UT WOS:000370637100001 PM 27313852 ER PT J AU Solano-Aguilar, G Molokin, A Botelho, C Fiorino, AM Vinyard, B Li, R Chen, C Urban, J Dawson, H Andreyeva, I Haverkamp, M Hibberd, PL AF Solano-Aguilar, Gloria Molokin, Aleksey Botelho, Christine Fiorino, Anne-Maria Vinyard, Bryan Li, Robert Chen, Celine Urban, Joseph, Jr. Dawson, Harry Andreyeva, Irina Haverkamp, Miriam Hibberd, Patricia L. TI Transcriptomic Profile of Whole Blood Cells from Elderly Subjects Fed Probiotic Bacteria Lactobacillus rhamnosus GG ATCC 53103 (LGG) in a Phase I Open Label Study SO PLOS ONE LA English DT Article ID NF-KAPPA-B; DIFFERENTIAL EXPRESSION ANALYSIS; PLACEBO-CONTROLLED TRIAL; RNA-SEQ DATA; SYNCYTIAL VIRUS-INFECTION; LACTIC-ACID BACTERIA; GENE-EXPRESSION; STATISTICAL-METHODS; CYTOKINE PRODUCTION; IMMUNE-RESPONSES AB We examined gene expression of whole blood cells (WBC) from 11 healthy elderly volunteers participating on a Phase I open label study before and after oral treatment with Lactobacillus rhamnosus GG-ATCC 53103 (LGG)) using RNA-sequencing (RNA-Seq). Elderly patients (65-80 yrs) completed a clinical assessment for health status and had blood drawn for cellular RNA extraction at study admission (Baseline), after 28 days of daily LGG treatment (Day 28) and at the end of the study (Day 56) after LGG treatment had been suspended for 28 days. Treatment compliance was verified by measuring LGG-DNA copy levels detected in host fecal samples. Normalized gene expression levels in WBC RNA were analyzed using a paired design built within three analysis platforms (edgeR, DESeq2 and TSPM) commonly used for gene count data analysis. From the 25,990 transcripts detected, 95 differentially expressed genes (DEGs) were detected in common by all analysis platforms with a nominal significant difference in gene expression at Day 28 following LGG treatment (FDR<0.1; 77 decreased and 18 increased). With a more stringent significance threshold (FDR< 0.05), only two genes (FCER2 and LY86), were down-regulated more than 1.5 fold and met the criteria for differential expression across two analysis platforms. The remaining 93 genes were only detected at this threshold level with DESeq2 platform. Data analysis for biological interpretation of DEGs with an absolute fold change of 1.5 revealed down-regulation of overlapping genes involved with Cellular movement, Cell to cell signaling interactions, Immune cell trafficking and Inflammatory response. These data provide evidence for LGG-induced transcriptional modulation in healthy elderly volunteers because pre-treatment transcription levels were restored at 28 days after LGG treatment was stopped. To gain insight into the signaling pathways affected in response to LGG treatment, DEG were mapped using biological pathways and genomic data mining packages to indicate significant biological relevance. C1 [Solano-Aguilar, Gloria; Molokin, Aleksey; Chen, Celine; Urban, Joseph, Jr.; Dawson, Harry] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. [Botelho, Christine; Fiorino, Anne-Maria; Andreyeva, Irina; Haverkamp, Miriam; Hibberd, Patricia L.] Massachusetts Gen Hosp, Div Global Hlth, Boston, MA 02114 USA. [Vinyard, Bryan] ARS, Stat Grp, Northeast Area, USDA, Beltsville, MD USA. [Li, Robert] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD USA. RP Solano-Aguilar, G (reprint author), ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. EM Gloria.SolanoAguilar@ars.usda.gov FU National Institutes of Health, National Center for Complementary and Alternative Medicine (NIH/NCCAM) [3U01AT002952, 3K24AT003683]; National Institute of Health, National Center for Advancing Translational Sciences (NIH/NCATS) [1UL1 TR001102]; United States Department of Agriculture, Agricultural Research Service (USDA-ARS) [8040-51000-058-03]; Harvard Catalyst FX Funding support was provided by National Institutes of Health, National Center for Complementary and Alternative Medicine (NIH/NCCAM); http://nccam.nih.gov; 3U01AT002952 (PLH) and 3K24AT003683 (PLH), the Harvard Catalyst and National Institute of Health, National Center for Advancing Translational Sciences (NIH/NCATS); http://www.ncats.nih.gov; 1UL1 TR001102 (PLH) and United States Department of Agriculture, Agricultural Research Service (USDA-ARS); http://www.ars.usda.gov; project no 8040-51000-058-03. The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of NCCAM or USDA. NIH/NCCAM Program Officer Linda Duffy, PhD, MPH, contributed to the structuring of the U01 study design, inclusion of validation approaches and implementations of protocols under phased regulatory approved IND. Other than Dr. Duffy's contributions, the funders had no role in study design, data collection and analysis, decision to publish or preparation of manuscript. NR 102 TC 2 Z9 2 U1 5 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 9 PY 2016 VL 11 IS 2 AR e0147426 DI 10.1371/journal.pone.0147426 PG 34 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DD6MN UT WOS:000370038400008 PM 26859761 ER PT J AU Xu, YF Bailey, E Spackman, E Li, T Wang, H Long, LP Baroch, JA Cunningham, FL Lin, XX Jarman, RG DeLiberto, TJ Wan, XF AF Xu, Yifei Bailey, Elizabeth Spackman, Erica Li, Tao Wang, Hui Long, Li-Ping Baroch, John A. Cunningham, Fred L. Lin, Xiaoxu Jarman, Richard G. DeLiberto, Thomas J. Wan, Xiu-Feng TI Limited Antigenic Diversity in Contemporary H7 Avian-Origin Influenza A Viruses from North America SO SCIENTIFIC REPORTS LA English DT Article ID PATTERN-RECOGNITION RECEPTORS; PHYLOGENETIC ANALYSIS; LOW-PATHOGENICITY; UNITED-STATES; INTERSPECIES TRANSMISSION; COMMERCIAL POULTRY; BRITISH-COLUMBIA; DOMESTIC POULTRY; INNATE IMMUNITY; WILD BIRDS AB Subtype H7 avian-origin influenza A viruses (AIVs) have caused at least 500 confirmed human infections since 2003 and culling of >75 million birds in recent years. Here we antigenically and genetically characterized 93 AIV isolates from North America (85 from migratory waterfowl [1976-2010], 7 from domestic poultry [1971-2012], and 1 from a seal [1980]). The hemagglutinin gene of these H7 viruses are separated from those from Eurasia. Gradual accumulation of nucleotide and amino acid substitutions was observed in the hemagglutinin of H7 AIVs from waterfowl and domestic poultry. Genotype characterization suggested that H7 AIVs in wild birds form diverse and transient internal gene constellations. Serologic analyses showed that the 93 isolates cross-reacted with each other to different extents. Antigenic cartography showed that the average antigenic distance among them was 1.14 units (standard deviation [SD], 0.57 unit) and that antigenic diversity among the H7 isolates we tested was limited. Our results suggest that the continuous genetic evolution has not led to significant antigenic diversity for H7 AIVs from North America. These findings add to our understanding of the natural history of IAVs and will inform public health decision-making regarding the threat these viruses pose to humans and poultry. C1 [Xu, Yifei; Bailey, Elizabeth; Wang, Hui; Long, Li-Ping; Wan, Xiu-Feng] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Spackman, Erica] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA USA. [Li, Tao; Lin, Xiaoxu; Jarman, Richard G.] Walter Reed Army Inst Res, Viral Dis Branch, Silver Spring, MD USA. [Baroch, John A.; DeLiberto, Thomas J.] USDA, Natl Wildlife Res Ctr, Wildlife Serv, Anim & Plant Hlth Inspect Serv, Ft Collins, CO USA. [Cunningham, Fred L.] USDA, Mississippi Field Stn, Natl Wildlife Res Ctr, Wildlife Serv,Anim & Plant Hlth Inspect Serv, Mississippi State, MS USA. RP Wan, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM wan@cvm.msstate.edu FU NIH/NIAID [R01 AI116744]; Department of Defense (DoD) Global Emerging Infections Surveillance and Response System (GEIS), a Division of the Armed Forces Health Surveillance Center [P0172] FX We thank Nan Zhao and Lucy Senter for technical assistance with this project. This project was supported partially by NIH/NIAID grant no. R01 AI116744 to XFW and by funding from the Department of Defense (DoD) Global Emerging Infections Surveillance and Response System (GEIS), a Division of the Armed Forces Health Surveillance Center grant no. P0172 to RGJ and XL. NR 70 TC 1 Z9 1 U1 1 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 9 PY 2016 VL 6 AR 20688 DI 10.1038/srep20688 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DD0MT UT WOS:000369614800001 PM 26858078 ER PT J AU Straub, JN Kaminski, RM Leach, AG Ezell, AW Leininger, T AF Straub, Jacob N. Kaminski, Richard M. Leach, Alan G. Ezell, Andrew W. Leininger, Theodor TI Acorn Yield and Masting Traits of Red Oaks in the Lower Mississippi River Alluvial Valley SO FOREST SCIENCE LA English DT Article DE Quercus spp.; Erythrobalanus; yield; oak reproduction; reproductive synchrony ID NORTH-AMERICAN OAKS; SEED PRODUCTION; SPATIAL SYNCHRONY; GROSS ENERGY; WOOD DUCKS; CONSERVATION; VARIABILITY; PATTERNS; ECOLOGY; WEATHER AB We studied five species of endemic red oaks (Section Erythrobalanus) in hardwood bottomlands of the Mississippi Alluvial Valley (MAV), including cherrybark, Nuttall, pin, water, and willow oaks. Ecologists and managers need reliable estimates of acorn yield and masting traits to assess potential forest regeneration and estimate foraging carrying capacity of these habitats for waterfowl and other wildlife. We designed a study to reliably estimate red oak acorn yield in five states in the Mississippi Alluvial Valley over 4 consecutive years (2009-2013) and evaluate the components of masting (i.e., synchrony and temporal variability). The mean annual individual tree yield was 424 kg (dry)/ha (35.1 acorns/m(2) crown area) across all sites and years. Our yield estimates provide heretofore unreported estimates of average seed yield (seeds/m(2) canopy) and mass (kg/ha) from red oaks for five of the largest contiguous bottomland hardwood forests remaining in the MAV. In our study, red oaks yielded more acorns on average and showed less annual variability than studies of other red oaks in North America. We found no evidence that annual population-level red oak acorn yield was synchronized at sites across the scale of the MAV. However, synchrony increased with decreasing distance between sites, and we found a large degree of interspecific synchronicity within four of our five sites. We believe that local scale influences such as edaphic resources, weather, and genetics drive within-site yearly patterns in acorn yield. Managers have a greater likelihood of lessening the effect of periodic seed failures in the MAV if they manage for a diversity of site-adapted red oak species as opposed to favoring monocultures or few species. C1 [Straub, Jacob N.] SUNY Coll Plattsburgh, Plattsburgh, NY 12901 USA. [Kaminski, Richard M.; Ezell, Andrew W.] Mississippi State Univ, Mississippi State, MS 39762 USA. [Leach, Alan G.] Univ Nevada, Reno, NV 89557 USA. [Leininger, Theodor] US Forest Serv, USDA, Washington, DC USA. RP Straub, JN (reprint author), SUNY Coll Plattsburgh, Plattsburgh, NY 12901 USA.; Kaminski, RM; Ezell, AW (reprint author), Mississippi State Univ, Mississippi State, MS 39762 USA.; Leach, AG (reprint author), Univ Nevada, Reno, NV 89557 USA.; Leininger, T (reprint author), US Forest Serv, USDA, Washington, DC USA. EM jstra009@plattsburgh.edu; rkaminski@cfr.msstate.edu; aleach@cabnr.unr.edu; aezell@cfr.msstate.edu; tleininger@fs.fed.us OI Leach, Alan/0000-0002-7645-8312 FU USDA Forest Service [07-JV-11330127-140]; US Fish and Wildlife Service under Region 4; Challenge Cost Share Grant [CS65]; Mississippi State University Forest and Wildlife Research Center FX Funding for this project was provided by the USDA Forest Service under Agreement 07-JV-11330127-140, and the US Fish and Wildlife Service under Region 4, Challenge Cost Share Grant CS65. We thank the Mississippi State University Forest and Wildlife Research Center for funding and additional support. We thank J. Foth, K. Wigen, E. Interis, N. Biasini, R. Thornton, J. Sloan, E. Boerger, and M. Groff for dedicated field and laboratory assistance. NR 67 TC 0 Z9 0 U1 2 U2 15 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB 8 PY 2016 VL 62 IS 1 BP 18 EP 27 DI 10.5849/forsci.14-152 PG 10 WC Forestry SC Forestry GA DC3SX UT WOS:000369140200003 ER PT J AU McDill, ME Toth, SF St John, R Braze, J Rebain, SA AF McDill, Marc E. Toth, Sandor F. St John, Rachel Braze, Janis Rebain, Stephanie A. TI Comparing Model I and Model II Formulations of Spatially Explicit Harvest Scheduling Models with Maximum Area Restrictions SO FOREST SCIENCE LA English DT Article DE forest planning models; mixed-integer programming; area-based adjacency constraints; area-restriction models ID FOREST PLANNING PROBLEMS; TABU SEARCH; ADJACENCY CONSTRAINTS; BOUND ALGORITHM; MANAGEMENT; SUBJECT AB This article investigates whether Johnson and Scheurman's (For. Sci. Monogr. 18, Society of American Foresters, Bethesda, MD 1977) Model II formulation, which can dramatically reduce the size and difficulty of linear programming harvest scheduling models, offers similar potential for efficiency gains in solving spatially explicit harvest scheduling models with area-based adjacency constraints. A total of 150 hypothetical problems and 10 real problems were formulated using Models I and II. The hypothetical problems were distributed (30 each) in five categories: regulated forest problems with four, six, and eight planning periods and overmature forest problems with four and six periods. The length of the planning horizon was a key factor determining the relative performance of Model I and Model II formulations in spatially explicit forest management planning problems. Results from the hypothetical problems suggest that Model I formulations outperform Model II formulations for four-period problems. However, Model II formulations perform significantly better than Model I formulations for problems with planning horizons of six and eight planning periods. Real forest results exhibit similar trends. C1 [McDill, Marc E.] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. [Toth, Sandor F.; St John, Rachel] Univ Washington, Seattle, WA 98195 USA. [Braze, Janis] Alberta Minist Environm & Sustainable Resource De, Edmonton, AB, Canada. [Rebain, Stephanie A.] US Forest Serv, USDA, Grand Rapids, MI USA. RP McDill, ME (reprint author), Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. EM mem14@psu.edu; toths@uw.edu; rachelstjohn1@gmail.com; janis.braze@gov.ab.ca; sarebain@fs.fed.us FU Pennsylvania Bureau of Forestry; University of Washington's Precision Forestry Coop FX We thank Dr. Pete Bettinger of the University of Georgia for sharing the Loblolly pine data for this experiment. Also thanks to the Pennsylvania Bureau of Forestry and the University of Washington's Precision Forestry Coop for the financial support of this study. NR 56 TC 1 Z9 1 U1 3 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB 8 PY 2016 VL 62 IS 1 BP 28 EP 37 DI 10.5849/forsci.14-179 PG 10 WC Forestry SC Forestry GA DC3SX UT WOS:000369140200004 ER PT J AU Xu, YL Cai, NAH Woeste, K Kang, XY He, CZ Li, GQ Chen, S Duan, AA AF Xu, Yulan Cai, Nianhui Woeste, Keith Kang, Xiangyang He, Chengzhong Li, Genqian Chen, Shi Duan, Anan TI Genetic Diversity and Population Structure of Pinus yunnanensis by Simple Sequence Repeat Markers SO FOREST SCIENCE LA English DT Article DE microsatellite; Yunnan pine; genetic structure; genetic diversity ID MULTILOCUS GENOTYPE DATA; TIBETAN PLATEAU; MICROSATELLITE MARKERS; CHLOROPLAST; INFERENCE; SOFTWARE; PLANTS; LOCI; MITOCHONDRIAL; CONSERVATION AB Pinus yunnanensis is the main species used for reforestation in Yunnan province, China. To manage the genetic resources of this economically and ecologically important species efficiently, greater knowledge of the distribution of its genetic diversity is needed. In this study, the genetic diversity of 20 natural populations representing most of the P. yunnanensis range was examined using seven simple sequence repeats. No loci showed significant departure from Hardy-Weinberg equilibrium. Most genetic diversity was found within populations, and overall genetic differentiation was low (F-ST = 0.045). There was no sign of isolation by distance. Clustering of populations using both neighbor joining pair groups and principal coordinates analysis based on Nei's genetic distance showed the presence of two major clusters. This result was reinforced using the Bayesian software STRUCTURE, which identified two southern populations as clearly separate from the others. There were no signs of genetic erosion, but some populations should be a focus of conservation efforts because of their higher level of genetic diversity and the presence of private alleles. These results will inform conservation and management of P. yunnanensis and will guide future studies of population genetics and breeding programs. C1 [Xu, Yulan; Cai, Nianhui; He, Chengzhong; Li, Genqian; Chen, Shi; Duan, Anan] Southwest Forestry Univ, Key Lab Forest Genet & Tree Improvement & Propaga, Kunming, Yunnan, Peoples R China. [Woeste, Keith] Purdue Univ, USDA, US Forest Serv, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Kang, Xiangyang] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, State Engn Lab Tree Breeding, Beijing, Peoples R China. RP Xu, YL (reprint author), Southwest Forestry Univ, Key Lab Forest Genet & Tree Improvement & Propaga, Kunming, Yunnan, Peoples R China.; Woeste, K (reprint author), Purdue Univ, USDA, US Forest Serv, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. EM xvyulan@163.com; woeste@purdue.edu FU Natural Science Foundation of China (NSFC) [31260191, 31360189] FX This study was supported by grants from the Natural Science Foundation of China (NSFC 31260191 and 31360189). This study was conducted in the Key Laboratory of Biodiversity Conservation in Southwest China (Southwest Forestry University), State Forestry administration. We are grateful to Jie Zeng and Junjie Guo for advice on the procedure of experiments and to Qingzhang Du for help on the data analysis. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 67 TC 1 Z9 2 U1 5 U2 19 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB 8 PY 2016 VL 62 IS 1 BP 38 EP 47 DI 10.5849/forsci.15-064 PG 10 WC Forestry SC Forestry GA DC3SX UT WOS:000369140200005 ER PT J AU Huang, YN Zhang, H Rogers, S Coggeshall, M Woeste, K AF Huang, Yen-Ning Zhang, Hao Rogers, Scott Coggeshall, Mark Woeste, Keith TI White Oak Growth after 23 Years in a Three-Site Provenance/Progeny Trial on a Latitudinal Gradient in Indiana SO FOREST SCIENCE LA English DT Article DE Quercus alba; forest genetics; mixed model; spatial autocorrelation; tree improvement ID FOREST GENETIC TRIALS; UNITED-STATES; PLANTATIONS; PARAMETERS; PERFORMANCE AB To increase the availability of improved, adapted white oak (Quercus alba L.) for midwestern United States landowners, we analyzed data from three 23-year-old provenance/progeny tests of 70 open-pollinated progenies from 17 provenances. Our goal was to estimate the heritability of height growth and range of adaptation and ultimately to determine the value of converting the sites to seed orchards. Tree growth was marked by positive spatial autocorrelation (SA) for height in all three test sites despite differences in management and mortality. Microsites with the highest SA changed little from age 10 to age 23. Nearest neighbor and iterative spatial or kriging analyses were used to remove effects of SA from the data, resulting in little change in heritability estimates but important changes in family means and rank. Within sites, provenances were a relatively unimportant source of variation (mostly <2%), and there was no evidence local sources grew best. Genetic correlation was 0.81 for height between ages 10 and 23. Considering heritability estimates, significant differences among families, and large predicted breeding zones, once the sites are thinned, seedlings produced from the progeny tests should grow well above average on suitable sites in Indiana and would probably be acceptable in nearby states as well. C1 [Huang, Yen-Ning; Zhang, Hao] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. [Rogers, Scott] US Forest Serv, Oconto River Seed Orchard, USDA, Reg 9, W Lafayette, IN USA. [Coggeshall, Mark] Univ Missouri Columbia, W Lafayette, IN USA. [Woeste, Keith] Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, USDA, Forest Serv,Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. RP Huang, YN; Zhang, H (reprint author), Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA.; Rogers, S (reprint author), US Forest Serv, Oconto River Seed Orchard, USDA, Reg 9, W Lafayette, IN USA.; Coggeshall, M (reprint author), Univ Missouri Columbia, W Lafayette, IN USA.; Woeste, K (reprint author), Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, USDA, Forest Serv,Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. EM yehuang@purdue.edu; zhanghao@purdue.edu; srrogers@fs.fed.us; coggeshallm@missouri.edu; Kwoeste@fs.fed.us FU Purdue University FX We thank B. Beheler and J. McKenna for their assistance in data collection and Mary Francis Mahalovich, David Gwaze, Sam Foster, and Paul Bloese for helpful comments on the article. We thank the Indiana Department of Natural Resources, Division of Forestry for maintenance of the sites and for access. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. Support for Y.-N.H. and S.R. provided by Purdue University. NR 33 TC 0 Z9 0 U1 10 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB 8 PY 2016 VL 62 IS 1 BP 99 EP 106 DI 10.5849/forsci.15-013 PG 8 WC Forestry SC Forestry GA DC3SX UT WOS:000369140200011 ER PT J AU Nelson, AS Bragg, DC AF Nelson, Andrew S. Bragg, Don C. TI Multidecadal Response of Naturally Regenerated Southern Pine to Early Competition Control and Commercial Thinning SO FOREST SCIENCE LA English DT Article DE Crossett Experimental Forest; herbaceous control; woody control; commercial thinning; Upper West Gulf Coastal Plain ID LOBLOLLY-PINE; UNITED-STATES; VEGETATION MANAGEMENT; STAND DYNAMICS; COASTAL-PLAIN; PLANTATIONS; GROWTH; PRODUCTIVITY; MIDROTATION; FORESTS AB Multidecadal responses to early competition control are poorly documented in naturally regenerated southern pine stands. This study examined the effects of the following early herbicide treatments in thinned southern pine stands through age 31: (1) no control (CK), (2) herbaceous vegetation control only (HC), (3) woody vegetation control only (WC), and (4) total (woody + herbaceous) vegetation control (TC). Previously reported (through age 13) early competition control effects on net (standing + harvested) pine growth and yield were sustained from ages 15-31, where the CK treatment produced the lowest net volume growth and yield and the WC treatment generated only slightly higher and not significantly different production. Over the decades, mean tree dbh and height were consistently the greatest in the HC and TC treatments, resulting in significantly higher merchantable and sawtimber growth and yield. The pattern of growth-and-yield gains through age 31 were similar to those of other studies, including planted pine stands, indicating the importance of early competition control in the attempt to increase naturally regenerated pine production. C1 [Nelson, Andrew S.] Univ Arkansas Monticello, Sch Forest Resources, Arkansas Forest Resources Ctr, Monticello, AR USA. [Nelson, Andrew S.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83843 USA. [Bragg, Don C.] US Forest Serv, USDA, Washington, DC USA. RP Nelson, AS (reprint author), Univ Arkansas Monticello, Sch Forest Resources, Arkansas Forest Resources Ctr, Monticello, AR USA.; Nelson, AS (reprint author), Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83843 USA.; Bragg, DC (reprint author), US Forest Serv, USDA, Washington, DC USA. EM asnelson@uidaho.edu; dbragg@fs.fed.us FU University of Arkansas, Division of Agriculture; Southern Research Station of the USDA Forest Service FX We thank Michael Cain and Dr. Michael Shelton for designing the study, implementing the treatments, collecting early measurements, and publishing the initial results. We also thank Kirby Sneed and Jess Riddle for collecting the age 31 measurements. Project funding was provided by the University of Arkansas, Division of Agriculture, and the Southern Research Station of the USDA Forest Service. NR 51 TC 0 Z9 0 U1 2 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB 8 PY 2016 VL 62 IS 1 BP 115 EP 124 DI 10.5849/forsci.15-056 PG 10 WC Forestry SC Forestry GA DC3SX UT WOS:000369140200013 ER PT J AU Rhoades, PR Koch, JB Waits, LP Strange, JP Eigenbrode, SD AF Rhoades, Paul R. Koch, Jonathan B. Waits, Lisette P. Strange, James P. Eigenbrode, Sanford D. TI Evidence for Bombus occidentalis (Hymenoptera: Apidae) Populations in the Olympic Peninsula, the Palouse Prairie, and Forests of Northern Idaho SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Bombus occidentalis; native bee conservation; native bee; Bombus conservation; pollinator ID BUMBLE BEES BOMBUS; POLLINATOR DECLINES; SPECIAL FOCUS; APIFORMES; CONSERVATION; IMPACTS AB Since the mid-1990s, Bombus occidentalis (Green) has declined from being one of the most common to one of the rarest bumble bee species in the Pacific Northwest of the United States. Although its conservation status is unresolved, a petition to list this species as endangered or threatened was recently submitted to the U.S. Fish and Wildlife Service. To shed light on the conservation situation and inform the U.S. Fish and Wildlife Service decision, we report on the detection and abundance of B. occidentalis following bumble bee collection between 2012 and 2014 across the Pacific Northwest. Collection occurred from the San Juan Islands and Olympic peninsula east to northern Idaho and northeastern Oregon, excluding the arid region in central Washington. B. occidentalis was observed at 23 collection sites out of a total of 234. With the exception of three sites on the Olympic peninsula, all of these were in the southeastern portion of the collection range. C1 [Rhoades, Paul R.; Eigenbrode, Sanford D.] Univ Idaho, Dept Plant Soil & Entomol Sci, 875 Perimeter Dr, Moscow, ID 83844 USA. [Koch, Jonathan B.] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. [Koch, Jonathan B.; Strange, James P.] Utah State Univ, USDA ARS, Pollinating Insects Res Unit, BNR 255, Logan, UT 84322 USA. [Waits, Lisette P.] Univ Idaho, Dept Fish & Wildlife Sci, 875 Perimeter Dr, Moscow, ID 83844 USA. RP Rhoades, PR (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, 875 Perimeter Dr, Moscow, ID 83844 USA. EM paul.r.rhoades@gmail.com; jonathan.b.koch@gmail.com; lwaits@uidaho.edu; james.strange@ars.usda.gov; sanforde@uidaho.edu FU National Science Foundation Integrated Graduate Education and Research Traineeship program; North Coast and Cascades Science Learning Network [NPS P13PG00149/FSN] FX We would like to thank the landowners of the Palouse Prairie who gave us access to prairie sites for insect collection, Dr. Sujaya Rao for her review of this manuscript, and Dr. Tim Hatten for general advice. Surveys in Pacific Northwest U.S. National Parks would not have been possible without the assistance of Dr. Brandon Hopkins, Craig Huntzinger, Houston Judd, Jason Long, Dr. Elinor Lichtenberg, Dr. Walter S. Sheppard, Dr. Chris Looney, and Joyce Knoblett. This project was supported through the National Science Foundation Integrated Graduate Education and Research Traineeship program (to P.R.R. and S.D.E.) and a research grant from the North Coast and Cascades Science Learning Network (NPS P13PG00149/FSN to J.P.S.). NR 31 TC 0 Z9 0 U1 7 U2 16 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD FEB 8 PY 2016 VL 16 DI 10.1093/jisesa/iev155 PG 5 WC Entomology SC Entomology GA DD3ZB UT WOS:000369861000001 ER PT J AU Li, RW Li, WZ Sun, JJ Yu, P Baldwin, RL Urban, JF AF Li, Robert W. Li, Weizhong Sun, Jiajie Yu, Peng Baldwin, Ransom L. Urban, Joseph F. TI The effect of helminth infection on the microbial composition and structure of the caprine abomasal microbiome SO SCIENTIFIC REPORTS LA English DT Article ID HAEMONCHUS-CONTORTUS; GUT MICROBIOTA; AMINO-ACIDS; DIVERSITY; LAMBS; PH; NUTRITION; SEQUENCES; ABILITY AB Haemonchus contortus is arguably the most injurious helminth parasite for small ruminants. We characterized the impact of H. contortus infection on the caprine abomasal microbiome. Fourteen parasite naive goats were inoculated with 5,000 H. contortus infective larvae and followed for 50 days. Six age-matched naive goats served as uninfected controls. Reduced bodyweight gain and a significant increase in the abosamal pH was observed in infected goats compared to uninfected controls. Infection also increased the bacterial load while reducing the abundance of the Archaea in the abomasum but did not appear to affect microbial diversity. Nevertheless, the infection altered the abundance of approximately 19% of the 432 species-level operational taxonomic units (OTU) detected per sample. A total of 30 taxa displayed a significantly different abundance between control and infected goats. Furthermore, the infection resulted in a distinct difference in the microbiome structure. As many as 8 KEGG pathways were predicted to be significantly affected by infection. In addition, H. contortus-induced changes in butyrate producing bacteria could regulate mucosal inflammation and tissue repair. Our results provided insight into physiological consequences of helminth infection in small ruminants and could facilitate the development of novel control strategies to improve animal and human health. C1 [Li, Robert W.; Sun, Jiajie; Baldwin, Ransom L.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Li, Weizhong] J Craig Venter Inst, La Jolla, CA 92037 USA. [Yu, Peng] Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA. [Yu, Peng] Texas A&M Univ, TEES AgriLife Ctr Bioinformat & Genom Syst Engn C, College Stn, TX 77843 USA. [Urban, Joseph F.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Sun, Jiajie] South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China. RP Li, RW (reprint author), ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM Robert.Li@ars.usda.gov RI Li, Weizhong/A-9735-2008 OI Li, Weizhong/0000-0003-1804-9403 FU USDA National Institute of Food and Agriculture [2013-67015-22957] FX We would like to thank Deborah Hebert for her technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer. WL were partially supported by the USDA National Institute of Food and Agriculture under Award No. 2013-67015-22957 to WL & RWL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 2 Z9 2 U1 6 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 8 PY 2016 VL 6 AR 20606 DI 10.1038/srep20606 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DD2WB UT WOS:000369781800001 PM 26853110 ER PT J AU Williams, A Kane, DA Ewing, PM Atwood, LW Jilling, A Li, M Lou, Y Davis, AS Grandy, AS Huerd, SC Hunter, MC Koide, RT Mortensen, DA Smith, RG Snapp, SS Spokas, KA Yannarell, AC Jordan, NR AF Williams, Alwyn Kane, Daniel A. Ewing, Patrick M. Atwood, Lesley W. Jilling, Andrea Li, Meng Lou, Yi Davis, Adam S. Grandy, A. Stuart Huerd, Sheri C. Hunter, Mitchell C. Koide, Roger T. Mortensen, David A. Smith, Richard G. Snapp, Sieglinde S. Spokas, Kurt A. Yannarell, Anthony C. Jordan, Nicholas R. TI Soil Functional Zone Management: A Vehicle for Enhancing Production and Soil Ecosystem Services in Row-Crop Agroecosystems SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE crop yield; ecosystem services; precision tillage; soil biodiversity; soil management; temporal intensification; trade-offs; zonal tillage ID ARBUSCULAR-MYCORRHIZAL FUNGI; NO-TILLAGE AGROECOSYSTEMS; ORGANIC-MATTER DYNAMICS; CARBON-USE EFFICIENCY; WINTER COVER CROPS; CONSERVATION TILLAGE; RIDGE-TILLAGE; LONG-TERM; NITROGEN MINERALIZATION; PHYSICAL-PROPERTIES AB There is increasing global demand for food, bioenergy feedstocks and a wide variety of bio-based products. In response, agriculture has advanced production, but is increasingly depleting soil regulating and supporting ecosystem services. New production systems have emerged, such as no-tillage, that can enhance soil services but may limit yields. Moving forward, agricultural systems must reduce trade-offs between production and soil services. Soil functional zone management (SFZM) is a novel strategy for developing sustainable production systems that attempts to integrate the benefits of conventional, intensive agriculture, and no-tillage. SFZM creates distinct functional zones within crop row and inter-row spaces. By incorporating decimeter-scale spatial and temporal heterogeneity, SFZM attempts to foster greater soil biodiversity and integrate complementary soil processes at the sub-field level. Such integration maximizes soil services by creating zones of 'active turnover', optimized for crop growth and yield (provisioning services); and adjacent zones of 'soil building', that promote soil structure development, carbon storage, and moisture regulation (regulating and supporting services). These zones allow SFZM to secure existing agricultural productivity while avoiding or minimizing trade-offs with soil ecosystem services. Moreover, the specific properties of SFZM may enable sustainable increases in provisioning services via temporal intensification (expanding the portion of the year during which harvestable crops are grown). We present a conceptual model of 'virtuous cycles', illustrating how increases in crop yields within SFZM systems could create self-reinforcing feedback processes with desirable effects, including mitigation of trade-offs between yield maximization and soil ecosystem services. Through the creation of functionally distinct but interacting zones, SFZM may provide a vehicle for optimizing the delivery of multiple goods and services in agricultural systems, allowing sustainable temporal intensification while protecting and enhancing soil functioning. C1 [Williams, Alwyn; Ewing, Patrick M.; Huerd, Sheri C.; Jordan, Nicholas R.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Kane, Daniel A.; Snapp, Sieglinde S.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Atwood, Lesley W.; Jilling, Andrea; Grandy, A. Stuart; Smith, Richard G.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Li, Meng; Lou, Yi; Yannarell, Anthony C.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL USA. [Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL USA. [Hunter, Mitchell C.; Mortensen, David A.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Koide, Roger T.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Spokas, Kurt A.] USDA ARS, Soil & Water Management Unit, St Paul, MN USA. RP Williams, A (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM alwyn.williams@outlook.com RI Spokas, Kurt/F-4839-2016; OI Spokas, Kurt/0000-0002-5049-5959; Hunter, Mitchell/0000-0002-4562-7806 FU Agriculture and Food Research Initiative (AFRI) Climate Change Mitigation and Adaptation in Agriculture grant [2011-67003-30343]; United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) FX This project was supported by an Agriculture and Food Research Initiative (AFRI) Climate Change Mitigation and Adaptation in Agriculture grant (2011-67003-30343), from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA). The use of trade, firm, or corporation names in this paper is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer. NR 193 TC 3 Z9 3 U1 11 U2 53 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 5 PY 2016 VL 7 AR 65 DI 10.3389/fpls.2016.00065 PG 15 WC Plant Sciences SC Plant Sciences GA DD2XH UT WOS:000369785000001 PM 26904043 ER PT J AU Zobel, RW AF Zobel, Richard W. TI Arabidopsis: An Adequate Model for Dicot Root Systems? SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Arabidopsis; roots; root classes; model plants; root system architecture (RSA) ID ARCHITECTURAL ANALYSIS; PLANT; THALIANA; MUTATION AB The Arabidopsis root system is frequently considered to have only three classes of root: primary, lateral, and adventitious. Research with other plant species has suggested up to eight different developmental/functional classes of root for a given plant root system. If Arabidopsis has only three classes of root, it may not be an adequate model for eudicot plant root systems. Recent research, however, can be interpreted to suggest that pre-flowering Arabidopsis does have at least five (5) of these classes of root. This then suggests that Arabidopsis root research can be considered an adequate model for dicot plant root systems. C1 [Zobel, Richard W.] ARS, Plant Sci Res Unit, USDA, Raleigh, NC USA. RP Zobel, RW (reprint author), ARS, Plant Sci Res Unit, USDA, Raleigh, NC USA. EM rich.zobel@ars.usda.gov FU U.S. Department of Agriculture FX Base funds received from U.S. Department of Agriculture. NR 16 TC 0 Z9 0 U1 3 U2 6 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 5 PY 2016 VL 7 AR 58 DI 10.3389/fpls.2016.00058 PG 3 WC Plant Sciences SC Plant Sciences GA DD2XA UT WOS:000369784300001 PM 26904040 ER PT J AU Nelson, SM Shay, AE James, JL Carlson, BA Urban, JF Prabhu, KS AF Nelson, Shakira M. Shay, Ashley E. James, Jannaal L. Carlson, Bradley A. Urban, Joseph F., Jr. Prabhu, K. Sandeep TI Selenoprotein Expression in Macrophages Is Critical for Optimal Clearance of Parasitic Helminth Nippostrongylus brasiliensis SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ALTERNATIVELY ACTIVATED MACROPHAGES; NEMATODE-INDUCED ALTERATIONS; RECEPTOR PPAR-GAMMA; GENE-EXPRESSION; UP-REGULATION; SELENIUM DEFICIENCY; MURINE MACROPHAGES; IMMUNE-RESPONSE; IN-VIVO; CELLS AB The plasticity of macrophages is evident in helminthic parasite infections, providing protection from inflammation. Previously we demonstrated that the micronutrient selenium induces a phenotypic switch in macrophage activation from a classically activated (pro-inflammatory; M1/CAM) toward an alternatively activated (anti-inflammatory; M2/AAM) phenotype, where cyclooxygenase (COX)-dependent cyclopentenone prostaglandin J(2) (15d-PGJ(2)) plays a key role. Here, we hypothesize that dietary selenium modulates macrophage polarization toward an AAM phenotype to assist in the increasing clearance of adult Nippostrongylus brasiliensis, a gastrointestinal nematode parasite. Mice on a selenium-adequate (0.08 ppm) diet significantly augmented intestinal AAM presence while decreasing adult worms and fecal egg production when compared with infection of mice on selenium-deficient (<0.01 ppm) diet. Further increase in dietary selenium to supraphysiological levels (0.4 ppm) had very little or no impact on worm expulsion. Normal adult worm clearance and enhanced AAM marker expression were observed in the selenium-supplemented Trsp(fl/fl)Cre(WT) mice that express selenoproteins driven by tRNA(Sec) (Trsp), whereas N. brasiliensis-infected Trsp(fl/fl)Cre(LysM) selenium-supplemented mice showed a decreased clearance, with lowered intestinal expression of several AAM markers. Inhibition of the COX pathway with indomethacin resulted in delayed worm expulsion in selenium-adequate mice. This was rescued with 15d-PGJ(2), which partially recapitulated the effect of selenium supplementation on fecal egg output in addition to increasing markers of AAMs in the small intestine. Antagonism of PPAR gamma blocked the effect of selenium. These results suggest that optimal expression of selenoproteins and selenium-dependent production of COX-derived endogenous prostanoids, such as Delta(12)-PGJ(2) and 15d-PGJ(2), may regulate AAM activation to enhance anti-helminthic parasite responses. C1 [Nelson, Shakira M.; Shay, Ashley E.; James, Jannaal L.; Prabhu, K. Sandeep] Penn State Univ, Ctr Mol Immunol & Infect Dis, University Pk, PA 16802 USA. [Nelson, Shakira M.; Shay, Ashley E.; James, Jannaal L.; Prabhu, K. Sandeep] Penn State Univ, Dept Vet & Biomed Sci, Ctr Mol Toxicol & Carcinogenesis, University Pk, PA 16802 USA. [Nelson, Shakira M.] NCI, Div Canc Epidemiol & Genet, NIH, Rockville, MD 20850 USA. [Carlson, Bradley A.] NCI, Mol Biol Selenium Sect, Mouse Canc Genet Program, NIH, Bethesda, MD 20892 USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. RP Prabhu, KS (reprint author), Penn State Univ, Dept Vet & Biomed Sci, 115 Henning Bldg, University Pk, PA 16802 USA. EM ksprabhu@psu.edu FU National Institutes of Health Public Health Service Grant [DK077152]; United States Department of Agriculture National Institute of Food and Agriculture Hatch Project [4475]; National Institutes of Health Predoctoral Training Grant [T32 AI074551]; Agricultural Research Service of the United States Department of Agriculture [1235-5100-058] FX This work was supported, in whole or in part, by National Institutes of Health Public Health Service Grant DK077152 and United States Department of Agriculture National Institute of Food and Agriculture Hatch Project 4475 (to K. S. P.), National Institutes of Health Predoctoral Training Grant T32 AI074551 (to A. E. S.), and the Agricultural Research Service of the United States Department of Agriculture (1235-5100-058) (to J. F. U.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. NR 49 TC 2 Z9 3 U1 0 U2 5 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD FEB 5 PY 2016 VL 291 IS 6 BP 2787 EP 2798 DI 10.1074/jbc.M115.684738 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DD0RK UT WOS:000369626900019 PM 26644468 ER PT J AU Gao, LL Turner, MK Chao, SM Kolmer, J Anderson, JA AF Gao, Liangliang Turner, M. Kathryn Chao, Shiaoman Kolmer, James Anderson, James A. TI Genome Wide Association Study of Seedling and Adult Plant Leaf Rust Resistance in Elite Spring Wheat Breeding Lines SO PLOS ONE LA English DT Article ID POPULATION-STRUCTURE; SELECTION; IMPROVEMENT; COLLECTION; SOFTWARE; PROGRAM; TRAITS; NUMBER; UG99; TOOL AB Leaf rust is an important disease, threatening wheat production annually. Identification of resistance genes or QTLs for effective field resistance could greatly enhance our ability to breed durably resistant varieties. We applied a genome wide association study (GWAS) approach to identify resistance genes or QTLs in 338 spring wheat breeding lines from public and private sectors that were predominately developed in the Americas. A total of 46 QTLs were identified for field and seedling traits and approximately 20-30 confer field resistance in varying degrees. The 10 QTLs accounting for the most variation in field resistance explained 26-30% of the total variation (depending on traits: percent severity, coefficient of infection or response type). Similarly, the 10 QTLs accounting for most of the variation in seedling resistance to different races explained 24-34% of the variation, after correcting for population structure. Two potentially novel QTLs (QLr. umn-1AL, QLr. umn-4AS) were identified. Identification of novel genes or QTLs and validation of previously identified genes or QTLs for seedling and especially adult plant resistance will enhance understanding of leaf rust resistance and assist breeding for resistant wheat varieties. We also developed computer programs to automate field and seedling rust phenotype data conversions. This is the first GWAS study of leaf rust resistance in elite wheat breeding lines genotyped with high density 90K SNP arrays. C1 [Gao, Liangliang; Turner, M. Kathryn; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Kolmer, James] USDA ARS Cereal Dis Lab, St Paul, MN 55108 USA. [Kolmer, James] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS Biosci Res Lab, Fargo, ND 58102 USA. RP Anderson, JA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA.; Kolmer, J (reprint author), USDA ARS Cereal Dis Lab, St Paul, MN 55108 USA.; Kolmer, J (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM Jim.Kolmer@ars.usda.gov; ander319@umn.edu OI Gao, Liangliang/0000-0002-3864-0631 FU United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) [2011-68002-30029] FX This study is part of the Triticeae Coordinated Agriculture Project (TCAP, www.triticeaecap.org), funded by the United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) grant no. 2011-68002-30029. NR 63 TC 4 Z9 4 U1 10 U2 33 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 5 PY 2016 VL 11 IS 2 AR e0148671 DI 10.1371/journal.pone.0148671 PG 25 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9PR UT WOS:000369554000153 PM 26849364 ER PT J AU Jefferson, A Leonard, H Siafarikas, A Woodhead, H Fyfe, S Ward, LM Munns, C Motil, K Tarquinio, D Shapiro, JR Brismar, T Ben-Zeev, B Bisgaard, AM Coppola, G Ellaway, C Freilinger, M Geerts, S Humphreys, P Jones, M Lane, J Larsson, G Lotan, M Percy, A Pineda, M Skinner, S Syhler, B Thompson, S Weiss, B Engerstrom, IW Downs, J AF Jefferson, Amanda Leonard, Helen Siafarikas, Aris Woodhead, Helen Fyfe, Sue Ward, Leanne M. Munns, Craig Motil, Kathleen Tarquinio, Daniel Shapiro, Jay R. Brismar, Torkel Ben-Zeev, Bruria Bisgaard, Anne-Marie Coppola, Giangennaro Ellaway, Carolyn Freilinger, Michael Geerts, Suzanne Humphreys, Peter Jones, Mary Lane, Jane Larsson, Gunilla Lotan, Meir Percy, Alan Pineda, Mercedes Skinner, Steven Syhler, Birgit Thompson, Sue Weiss, Batia Engerstrom, Ingegerd Witt Downs, Jenny TI Clinical Guidelines for Management of Bone Health in Rett Syndrome Based on Expert Consensus and Available Evidence SO PLOS ONE LA English DT Article ID X-RAY ABSORPTIOMETRY; VITAMIN-D DEFICIENCY; MINERAL DENSITY; QUANTITATIVE ULTRASOUND; POSITION STATEMENT; OFFICIAL POSITIONS; ENDOCRINE-SOCIETY; PUBERTAL CHANGES; CEREBRAL-PALSY; MECP2 MUTATION AB Objectives We developed clinical guidelines for the management of bone health in Rett syndrome through evidence review and the consensus of an expert panel of clinicians. Methods An initial guidelines draft was created which included statements based upon literature review and 11 open-ended questions where literature was lacking. The international expert panel reviewed the draft online using a 2-stage Delphi process to reach consensus agreement. Items describe the clinical assessment of bone health, bone mineral density assessment and technique, and pharmacological and non-pharmacological interventions. Results Agreement was reached on 39 statements which were formulated from 41 statements and 11 questions. When assessing bone health in Rett syndrome a comprehensive assessment of fracture history, mutation type, prescribed medication, pubertal development, mobility level, dietary intake and biochemical bone markers is recommended. A baseline densitometry assessment should be performed with accommodations made for size, with the frequency of surveillance determined according to individual risk. Lateral spine x-rays are also suggested. Increasing physical activity and initiating calcium and vitamin D supplementation when low are the first approaches to optimizing bone health in Rett syndrome. If individuals with Rett syndrome meet the ISCD criterion for osteoporosis in children, the use of bisphosphonates is recommended. Conclusion A clinically significant history of fracture in combination with low bone densitometry findings is necessary for a diagnosis of osteoporosis. These evidence and consensus-based guidelines have the potential to improve bone health in those with Rett syndrome, reduce the frequency of fractures, and stimulate further research that aims to ameliorate the impacts of this serious comorbidity. C1 [Jefferson, Amanda] Curtin Univ, Sch Biomed Sci, Curtin Hlth Innovat Res Inst Biosci, Perth, WA 6845, Australia. [Leonard, Helen; Downs, Jenny] Univ Western Australia, Telethon Kids Inst, Ctr Child Hlth Res, Perth, WA, Australia. [Siafarikas, Aris] Princess Margaret Childrens Hosp, Dept Endocrinol & Diabet, Perth, WA, Australia. [Woodhead, Helen] Sydney Childrens Hosp, Dept Paediat Endocrinol, Randwick, NSW, Australia. [Fyfe, Sue] Curtin Univ, Fac Hlth Sci, Perth, WA 6845, Australia. [Ward, Leanne M.] Univ Ottawa, Dept Pediat, Fac Med, Ottawa, ON K1N 6N5, Canada. [Ward, Leanne M.] Childrens Hosp Eastern Ontario, Div Endocrinol & Metab, Ottawa, ON K1H 8L1, Canada. [Munns, Craig] Childrens Hosp Westmead, Inst Endocrinol & Diabet, Sydney, NSW, Australia. [Motil, Kathleen] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [Motil, Kathleen] Texas Childrens Hosp, Sect Gastroenterol Hepatol & Nutr, Houston, TX 77030 USA. [Tarquinio, Daniel] Emory Univ, Childrens Healthcare Atlanta, Atlanta, GA 30322 USA. [Shapiro, Jay R.] Kennedy Krieger Inst, Bone & Osteogenesis Imperfecta Dept, Baltimore, MD USA. [Brismar, Torkel] Karolinska Inst, Dept Clin Sci Intervent & Technol, Stockholm, Sweden. [Ben-Zeev, Bruria] Edmond & Lily Safra Childrens Hosp, Pediat Neurol Unit, Chaim Sheba Med Ctr, Tel Hashomer, Israel. [Bisgaard, Anne-Marie; Syhler, Birgit] Ctr Rett Syndrome, Copenhagen, Denmark. [Bisgaard, Anne-Marie; Syhler, Birgit] Rigshosp, Dept Clin Genet, DK-2100 Copenhagen, Denmark. [Coppola, Giangennaro] Univ Salerno, Clin Child & Adolescent Neuropsychiat, Dept Med & Surg, I-84100 Salerno, Italy. [Ellaway, Carolyn] Childrens Hosp Westmead, Western Sydney Genet Program, Sydney, NSW, Australia. [Ellaway, Carolyn] Univ Sydney, Discipline Paediat & Child Hlth, Sydney, NSW 2006, Australia. [Ellaway, Carolyn] Univ Sydney, Discipline Genet Med, Sydney, NSW 2006, Australia. [Freilinger, Michael] Med Univ Vienna, Dept Pediat & Adolescent Med, Vienna, Austria. [Geerts, Suzanne; Lane, Jane] Univ Alabama Birmingham, Civitan Int Res Ctr, Birmingham, AL USA. [Humphreys, Peter] Childrens Hosp Eastern Ontario, Div Neurol, Ottawa, ON K1H 8L1, Canada. [Jones, Mary] UCSF Benioff Childrens Hosp, Katies Clin Rett Syndrome & Related Disorders, Oakland, CA USA. [Larsson, Gunilla] Swedish Natl Rett Ctr, Froson, Sweden. [Larsson, Gunilla] Umea Univ, Dept Community Med & Rehabil, Physiotherapy, Froson, Sweden. [Lotan, Meir] Ariel Univ, Dept Physiotherapy, Ariel, Israel. [Percy, Alan] Univ Alabama Birmingham, Dept Pediat & Neurol, Birmingham, AL USA. [Pineda, Mercedes] Fundacio Hosp St Joan Deu, Barcelona, Spain. [Pineda, Mercedes] Inst Salud Carlos III, Ctr Biomed Res Rare Dis, Barcelona, Spain. [Skinner, Steven] Greenwood Genet Ctr, Greenwood, SC 29646 USA. [Thompson, Sue] Childrens Hosp Westmead, Genet Metab Disorders Serv, Sydney, NSW, Australia. [Weiss, Batia] Edmond & Lily Safra Childrens Hosp, Div Pediat Gastroenterol & Nutr, Tel Hashomer, Israel. [Weiss, Batia] Chaim Sheba Med Ctr, IL-52621 Tel Hashomer, Israel. [Engerstrom, Ingegerd Witt] Swedish Natl Rett Ctr, Neuropediatr, Froson, Sweden. [Downs, Jenny] Curtin Univ, Sch Physiotherapy & Exercise Sci, Perth, WA 6845, Australia. RP Downs, J (reprint author), Univ Western Australia, Telethon Kids Inst, Ctr Child Hlth Res, Perth, WA, Australia. EM Jenny.Downs@telethonkids.org.au FU Rett Syndrome Association UK; National Institutes of Health [5R01HD043100-05]; National Medical and Health Research Council (NHMRC) [303189, 1004384]; Rett syndrome.org.; NIH; NHMRC; International Rett Syndrome Foundation FX Funding for this project was provided by the Rett Syndrome Association UK. The Australian Rett Syndrome program has previously been funded by the National Institutes of Health (5R01HD043100-05) and also the National Medical and Health Research Council (NHMRC) project grant #303189 and #1004384 for certain clinical aspects. The International Rett Syndrome Research Program is funded by the Rett syndrome.org.; The authors wish to thank our panel of parents from the Australian Consumer Reference Group for their useful insights and the International Rett Syndrome Foundation, Rettsyndrome.org for organising the listserv RettNet. We would also like to thank the UK for funding this stud, the NIH and NHMRC for funding the Australian Rett Syndrome research program, the NIH for funding the Natural History Study in the US and the International Rett Syndrome Foundation for funding InterRett research program. The authors also acknowledge the valuable contributions of the expert panel who participated in the Delphi technique. The expert panel comprised: Chhavi Agarwal, MD, MS, MRCP, FAAP, Pediatric Endocrinology of New York, New York, USA; Arthur Beisang, MD, Gillette Children's Hospital, St.; Paul, Minnesota, USA; Bruria Ben-Zeev, MD, Pediatric Neurology Unit, Edmond & Lily Safra Children's Hospital, Chaim Sheba Medical Center, Tel Hashomer, Israel; Anne-Marie Bisgaard, MD, PhD, Center for Rett Syndrome, Department of Clinical Genetics, Rigshospitalet, Copenhagen, Denmark; Torkel Brismar, MD, PhD, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Solna, Stockholm, Sweden; Angus Clarke, DM, FRCP, School of Medicine, Medical genetics, Cardiff University, Wales, UK; Giangennaro Coppola, MD, Clinic of Child and Adolescent Neuropsychiatry, Department of Medicine and Surgery, University of Salerno, Salerno, Italy; Matthew Drake, MD, PhD, Endocrinology, Diabetes, Metabolism and Nutrition Department, Mayo Clinic, Minnesota, USA; Carolyn Ellaway, MBBS, PhD, FRACP, Western Sydney Genetics Program, The Children's Hospital at Westmead, Disciplines of Paediatrics and Child Health and Genetic Medicine, University of Sydney, Sydney, New South Wales, Australia; Michael Freilinger, MD, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria; Suzanne Geerts, RD, MS, Civitan International Research Center, University of Alabama at Birmingham, Birmingham, Alabama, USA; Richard Haas, MD, Department of Neurosciences, University of California San Diego, La Jolla, California, USA; Gabriele Haeusler, MD, Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Italy; Ingrid Holm, MD, MPH, Division of genetics and Genomics, Boston Children's Hospital, Boston, USA; Peter Humphreys, MD, FRCP, Division of Neurology, Children's Hospital of Eastern Ontario, Ottawa, Ontario, Canada; Mary Jones, MD, MPH, Katie's Clinic for Rett syndrome and related disorders, UCSF Benioff Children's Hospital, Oakland, California, USA; Walter Kaufmann, MD, Centre for Genetic Disorders of Cognition & Behavior, Kennedy Krieger Institute, School of Medicine, Johns Hopkins University, Baltimore; Salman Kirmani, BS, MBBS, MD, Mayo Clinic, Rochester, New York, USA; Jane Lane, RN BSN, Civitan International Research Center, University of Alabama at Birmingham, Birmingham, Alabama, USA; Gunilla Larsson, PhD, Swedish Rett Centre, Froson, Sweden; Agnes Linglart, MD, French Institute of Health and Medical Research, Pediatric Endocrinology and Diabetes Service, Bicetre Hospital, Paris, France; Meir Lotan, PhD, Department of Physiotherapy, Ariel University, Ariel, Israel; Kathleen Motil, MD, PhD, Department of Pediatrics, USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, Section of Gastroenterology, Hepatology and Nutrition, Texas Children's Hospital, Houston, Texas, USA; Craig Munns, MBBS, PhD, FRACP, Institute of Endocrinology and Diabetes, The Children's Hospital at Westmead, Sydney, New South Wales, Australia; Yoshiko Nomura, MD, Segawa Neurological Clinic For Children, Tokyo, Japan; Alan Percy, MD, Department of Pediatrics and Neurology, University of Alabama at Birmingham, Birmingham, Alabama, USA; Mercedes Pineda, MD, PhD, Fundacio Hospital Sant Joan de Deu, Centre for Biomedical Research on Rare Diseases, Instituto de Salud Carlos III, Barcelona, Spain; Carolyn Schanen, MD, PhD, Nemours Biomedical Research, Alfred I. duPont Hosptial for Children, Wilmington, DE, USA; Jay Shapiro, MD, Bone and Osteogenesis Imperfecta Department, Kennedy Krieger Institute, Baltimore, MD, USA; Kevin Sheridan, MD, Gillette Children's Hospital, St.; Paul, Minnesota, USA; Steven Skinner, MD, Greenwood Genetic Center, Greenwood, South Carolina, USA; Bronwyn Stuckey, BA, MBBS, FRACP, Keogh Institute for Medical Research, Perth, WA, Australia; Birgit Syhler, BSc, Center for Rett Syndrome, Department of Clinical Genetics, Rigshospitalet, Copenhagen, Denmark; Daniel Tarquinio, DO, MS, Children's Healthcare of Atlanta, Emory University, Atlanta, Georgia, USA; Sue Thompson, BSc, AdvAPD, Genetic Metabolic Disorders Service, The Children's Hospital at Westmead, Sydney, New South Wales, Australia; Leanne Ward, MD, FRCPC, Research Chair in Pediatric Bone Health, Department of Pediatrics, Faculty of Medicine, University of Ottawa, Division of Endocrinology and Metabolism, Children's Hospital of Eastern Ontario, Ottawa, Canada; Batia Weiss, MD, Division of Pediatric Gastroenterology and Nutrition, Edmond & Lily Safra Children's Hospital, Chaim Sheba Medical Center, Tel Hashomer, Israel; Ingegerd Witt Engerstrom, MD, PhD, Swedish National Rett Center, Froson, Sweden. NR 68 TC 5 Z9 5 U1 2 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 5 PY 2016 VL 11 IS 2 AR e0146824 DI 10.1371/journal.pone.0146824 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9PR UT WOS:000369554000010 PM 26849438 ER PT J AU Kim, WY Peever, TL Park, JJ Park, CM Gang, DR Xian, M Davidson, JA Infantino, A Kaiser, WJ Chen, WD AF Kim, Wonyong Peever, Tobin L. Park, Jeong-Jin Park, Chung-Min Gang, David R. Xian, Ming Davidson, Jenny A. Infantino, Alessandro Kaiser, Walter J. Chen, Weidong TI Use of metabolomics for the chemotaxonomy of legume-associated Ascochyta and allied genera SO SCIENTIFIC REPORTS LA English DT Article ID PHOMA-MEDICAGINIS; HOST-SPECIFICITY; METABOLITE PROFILES; ALTERNARIA-SOLANI; DIDYMELLA-RABIEI; PISUM-SATIVUM; CAUSAL AGENT; 1ST REPORT; LEAF-SPOT; BLIGHT AB Chemotaxonomy and the comparative analysis of metabolic features of fungi have the potential to provide valuable information relating to ecology and evolution, but have not been fully explored in fungal biology. Here, we investigated the chemical diversity of legume-associated Ascochyta and Phoma species and the possible use of a metabolomics approach using liquid chromatography-mass spectrometry for their classification. The metabolic features of 45 strains including 11 known species isolated from various legumes were extracted, and the datasets were analyzed using chemometrics methods such as principal component and hierarchical clustering analyses. We found a high degree of intra-species consistency in metabolic profiles, but inter-species diversity was high. Molecular phylogenies of the legume-associated Ascochyta/Phoma species were estimated using sequence data from three protein-coding genes and the five major chemical groups that were detected in the hierarchical clustering analysis were mapped to the phylogeny. Clusters based on similarity of metabolic features were largely congruent with the species phylogeny. These results indicated that evolutionarily distinct fungal lineages have diversified their metabolic capacities as they have evolved independently. This whole metabolomics approach may be an effective tool for chemotaxonomy of fungal taxa lacking information on their metabolic content. C1 [Kim, Wonyong; Peever, Tobin L.; Chen, Weidong] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Park, Jeong-Jin; Gang, David R.] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA. [Park, Chung-Min; Xian, Ming] Washington State Univ, Dept Chem, Pullman, WA 99164 USA. [Davidson, Jenny A.] South Australian Res & Dev Inst, Adelaide, SA 5001, Australia. [Infantino, Alessandro] Consiglio Ric Agr & Anal Econ Agr CREA, Ctr Ric Patol Vegetale, I-00156 Rome, Italy. [Kaiser, Walter J.] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. [Chen, Weidong] Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. RP Chen, WD (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA.; Chen, WD (reprint author), Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. EM w-chen@wsu.edu FU National Science Foundation [DBI-1229749]; USDA Cool Season Food Legume Research Program; USA Dry Pea and Lentil Council FX We are grateful to Dr. H. Oikawa (Hokkaido University) for providing solanapyrone A standard. Mass spectrometric analysis was performed on an instrument acquired through a Major Research Instrumentation grant (DBI-1229749) from the National Science Foundation to D.R.G. The research was funded in part by the USDA Cool Season Food Legume Research Program and by the USA Dry Pea and Lentil Council. NR 64 TC 2 Z9 2 U1 4 U2 25 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 5 PY 2016 VL 6 AR 20192 DI 10.1038/srep20192 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC7FJ UT WOS:000369384300004 PM 26847260 ER PT J AU Dassanayake, RP Madsen-Bouterse, SA Truscott, TC Zhuang, DY Mousel, MR Davis, WC Schneider, DA AF Dassanayake, Rohana P. Madsen-Bouterse, Sally A. Truscott, Thomas C. Zhuang, Dongyue Mousel, Michelle R. Davis, William C. Schneider, David A. TI Classical scrapie prions are associated with peripheral blood monocytes and T-lymphocytes from naturally infected sheep SO BMC VETERINARY RESEARCH LA English DT Article DE Bioassay; Classical scrapie; Lambs; Monocytes; T lymphocytes ID LYMPHOID-TISSUE; PROTEIN; PRPSC; IMMUNOHISTOCHEMISTRY; AMPLIFICATION; ACCUMULATION; PATHOGENESIS; DIAGNOSIS; CELLS; FLOCK AB Background: Classical scrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats. Our previous bioassay studies in lambs revealed that scrapie prions could be detected in association with peripheral blood monocular cells (PBMC), B lymphocytes and platelet-rich plasma fractions. In the present study, bioassay in lambs was again used to determine if scrapie prions are associated with the other two subsets of PBMC, monocytes and T lymphocytes. Results: PBMC, monocytes and T lymphocytes were isolated from two preclinically affected VRQ/VRQ sheep naturally infected with classical ovine scrapie and intravenously transfused into VRQ/VRQ lambs post-weaning. As determined using standard immunohistochemistry for scrapie, abnormal isoforms of prion protein were detected in lymphoid tissues of lambs inoculated with PBMC (4/4 recipient lambs), monocytes (2/5) and T lymphocytes (1/4). Prion protein misfolding activity was detected by serial protein misfolding cyclic amplification (sPMCA) in PBMC from monocyte and T lymphocyte recipient sheep in agreement with antemortem rectal biopsy results, but such prion protein misfolding activity was not detected from other recipients. Conclusions: These findings show that scrapie prions are associated with monocytes and T lymphocytes circulating in the peripheral blood of sheep naturally infected with classical scrapie. Combined with our previous findings, we can now conclude that all three major subsets of PBMC can harbor prions during preclinical disease and thus, present logical targets for development of a sensitive assay to detect scrapie prions. In this regard, we have also demonstrated that sPMCA can be used to detect scrapie prions associated with PBMC. C1 [Dassanayake, Rohana P.; Madsen-Bouterse, Sally A.; Davis, William C.; Schneider, David A.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Truscott, Thomas C.; Zhuang, Dongyue; Mousel, Michelle R.; Schneider, David A.] USDA, Anim Dis Res Unit, Agr Res Serv, Pullman, WA 99164 USA. RP Dassanayake, RP (reprint author), Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM rohana1@vetmed.wsu.edu OI Mousel, Michelle/0000-0003-1367-7005 FU USDA Agricultural Research Service [CRIS 2090-32000-030-00D] FX This work was supported by funds from the USDA Agricultural Research Service (CRIS 2090-32000-030-00D). We would like to thank Seth Truscott and Katherine O'Rourke for critical reading of the manuscript. The authors would also like to thank Linda Hamburg and Lori Fuller for their excellent support during the blood transfusion experiments; Desiree Lesiak for PRNP genotyping; Lori Fuller, Deborah Wolheter, Jan Luft and Laetisha O'Rourke for care of the animals. We like to thank Washington Animal Disease Diagnostic Laboratory personnel at the histology laboratory for use of their tissue processor. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 26 TC 1 Z9 1 U1 1 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD FEB 4 PY 2016 VL 12 AR 27 DI 10.1186/s12917-016-0651-6 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DC6DN UT WOS:000369310100002 PM 26847623 ER PT J AU Cassmann, E White, R Atherly, T Wang, C Sun, YX Khoda, S Mosher, C Ackermann, M Jergens, A AF Cassmann, Eric White, Robin Atherly, Todd Wang, Chong Sun, Yaxuan Khoda, Samir Mosher, Curtis Ackermann, Mark Jergens, Albert TI Alterations of the Ileal and Colonic Mucosal Microbiota in Canine Chronic Enteropathies SO PLOS ONE LA English DT Article ID INFLAMMATORY-BOWEL-DISEASE; INVASIVE ESCHERICHIA-COLI; IN-SITU HYBRIDIZATION; MOLECULAR-PHYLOGENETIC CHARACTERIZATION; TARGETED OLIGONUCLEOTIDE PROBES; CROHNS-DISEASE; GRANULOMATOUS COLITIS; E. COLI; DOGS; BACTERIA AB Background The intestinal microbiota is increasingly linked to the pathogenesis of chronic enteropathies (CE) in dogs. While imbalances in duodenal and fecal microbial communities have been associated with mucosal inflammation, relatively little is known about alterations in mucosal bacteria seen with CE involving the ileum and colon. Aim To investigate the composition and spatial organization of mucosal microbiota in dogs with CE and controls. Methods Tissue sections from endoscopic biopsies of the ileum and colon from 19 dogs with inflammatory bowel disease (IBD), 6 dogs with granulomatous colitis (GC), 12 dogs with intestinal neoplasia, and 15 controls were studied by fluorescence in situ hybridization (FISH) on a quantifiable basis. Results The ileal and colonic mucosa of healthy dogs and dogs with CE is predominantly colonized by bacteria localized to free and adherent mucus compartments. CE dogs harbored more (P < 0.05) mucosal bacteria belonging to the Clostridium-coccoides/Eubacterium rectale group, Bacteroides, Enterobacteriaceae, and Escherichia coli versus controls. Within the CE group, IBD dogs had increased (P < 0.05) Enterobacteriaceae and E. coli bacteria attached onto surface epithelia or invading within the intestinal mucosa. Bacterial invasion with E. coli was observed in the ileal and colonic mucosa of dogs with GC (P < 0.05). Dogs with intestinal neoplasia had increased (P < 0.05) adherent (total bacteria, Enterobacteriaceae, E. coli) and invasive (Enterobacteriaceae, E. coli, and Bacteroides) bacteria in biopsy specimens. Increased numbers of total bacteria adherent to the colonic mucosa were associated with clinical disease severity in IBD dogs (P < 0.05). Conclusion Pathogenic events in canine CE are associated with different populations of the ileal and colonic mucosal microbiota. C1 [Cassmann, Eric; White, Robin; Khoda, Samir; Jergens, Albert] Iowa State Univ, Dept Vet Clin Sci, Coll Vet Med, Ames, IA USA. [Atherly, Todd] ARS, USDA, Ames, IA USA. [Wang, Chong; Sun, Yaxuan] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Coll Vet Med, Ames, IA USA. [Mosher, Curtis] Iowa State Univ, Coll Liberal Arts & Sci, Dept Genet Dev & Cell Biol, Ames, IA USA. [Ackermann, Mark] Iowa State Univ, Dept Vet Pathol, Coll Vet Med, Ames, IA USA. RP Jergens, A (reprint author), Iowa State Univ, Dept Vet Clin Sci, Coll Vet Med, Ames, IA USA. EM ajergens@iastate.edu RI Cassmann, Eric/B-2749-2016 OI Cassmann, Eric/0000-0002-7130-2650 NR 49 TC 1 Z9 1 U1 2 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 3 PY 2016 VL 11 IS 2 AR e0147321 DI 10.1371/journal.pone.0147321 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9OF UT WOS:000369550200032 PM 26840462 ER PT J AU Guseman, AJ Miller, K Kunkle, G Dively, GP Pettis, JS Evans, JD vanEngelsdorp, D Hawthorne, DJ AF Guseman, Alex J. Miller, Kaliah Kunkle, Grace Dively, Galen P. Pettis, Jeffrey S. Evans, Jay D. vanEngelsdorp, Dennis Hawthorne, David J. TI y Multi-Drug Resistance Transporters and a Mechanism-Based Strategy for Assessing Risks of Pesticide Combinations to Honey Bees SO PLOS ONE LA English DT Article ID GLYCOPROTEIN-MEDIATED TRANSPORT; P-GLYCOPROTEIN; MALPIGHIAN TUBULES; ABC TRANSPORTERS; TRANSEPITHELIAL TRANSPORT; INSECTICIDE TOXICITY; DRUG INTERACTIONS; APIS-MELLIFERA; EXPRESSION; IVERMECTIN AB Annual losses of honey bee colonies remain high and pesticide exposure is one possible cause. Dangerous combinations of pesticides, plant-produced compounds and antibiotics added to hives may cause or contribute to losses, but it is very difficult to test the many combinations of those compounds that bees encounter. We propose a mechanism-based strategy for simplifying the assessment of combinations of compounds, focusing here on compounds that interact with xenobiotic handling ABC transporters. We evaluate the use of ivermectin as a model substrate for these transporters. Compounds that increase sensitivity of bees to ivermectin may be inhibiting key transporters. We show that several compounds commonly encountered by honey bees (fumagillin, Pristine, quercetin) significantly increased honey bee mortality due to ivermectin and significantly reduced the LC50 of ivermectin suggesting that they may interfere with transporter function. These inhibitors also significantly increased honey bees sensitivity to the neonicotinoid insecticide acetamiprid. This mechanism-based strategy may dramatically reduce the number of tests needed to assess the possibility of adverse combinations among pesticides. We also demonstrate an in vivo transporter assay that provides physical evidence of transporter inhibition by tracking the dynamics of a fluorescent substrate of these transporters (Rhodamine B) in bee tissues. Significantly more Rhodamine B remains in the head and hemolymph of bees pretreated with higher concentrations of the transporter inhibitor verapamil. Mechanism-based strategies for simplifying the assessment of adverse chemical interactions such as described here could improve our ability to identify those combinations that pose significantly greater risk to bees and perhaps improve the risk assessment protocols for honey bees and similar sensitive species. C1 [Guseman, Alex J.; Miller, Kaliah; Kunkle, Grace; Dively, Galen P.; vanEngelsdorp, Dennis; Hawthorne, David J.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Guseman, Alex J.] Univ N Carolina, Dept Chem, Chapel Hill, NC USA. [Pettis, Jeffrey S.; Evans, Jay D.] ARS, Bee Res Lab, USDA, Beltsville, MD USA. RP Hawthorne, DJ (reprint author), Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. EM djh@umd.edu FU USDA-ARS Bee Research Laboratory; Maryland Agricultural Experiment Station; UMD Libraries Open Access Publishing Fund; National Socio-Environmental Synthesis Center (SESYNC)-National Science Foundation (NSF) [DBI-1052875] FX This work was funded primarily by a Cooperative Agreement with the USDA-ARS Bee Research Laboratory, and received partial funding by the Maryland Agricultural Experiment Station. Funding for Open Access was provided by the UMD Libraries Open Access Publishing Fund. D.J.H. received support from the National Socio-Environmental Synthesis Center (SESYNC)-National Science Foundation (NSF) award DBI-1052875. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 68 TC 0 Z9 0 U1 6 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 3 PY 2016 VL 11 IS 2 AR e0148242 DI 10.1371/journal.pone.0148242 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9OF UT WOS:000369550200109 PM 26840460 ER PT J AU Huang, Q Chen, YP Wang, RW Cheng, S Evans, JD AF Huang, Qiang Chen, Yan Ping Wang, Rui Wu Cheng, Shang Evans, Jay D. TI Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees SO PLOS ONE LA English DT Article ID NOSEMA-CERANAE MICROSPORIDIA; APIS-MELLIFERA; ENCEPHALITOZOON MICROSPORIDIA; PARASITOPHOROUS VACUOLE; APOPTOSIS; CELL; INFECTIONS; PROTEIN; MITOCHONDRIA; DROSOPHILA AB To clarify the mechanisms of Nosema ceranae parasitism, we deep-sequenced both honey bee host and parasite mRNAs throughout a complete 6-day infection cycle. By time-series analysis, 1122 parasite genes were significantly differently expressed during the reproduction cycle, clustering into 4 expression patterns. We found reactive mitochondrial oxygen species modulator 1 of the host to be significantly down regulated during the entire infection period. Our data support the hypothesis that apoptosis of honey bee cells was suppressed during infection. We further analyzed genome-wide genetic diversity of this parasite by comparing samples collected from the same site in 2007 and 2013. The number of SNP positions per gene and the proportion of non-synonymous substitutions per gene were significantly reduced over this time period, suggesting purifying selection on the parasite genome and supporting the hypothesis that a subset of N. ceranae strains might be dominating infection. C1 [Huang, Qiang; Wang, Rui Wu] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China. [Huang, Qiang; Chen, Yan Ping; Evans, Jay D.] ARS, USDA, Bee Res Lab, BARC East Bldg 306, Beltsville, MD 20705 USA. [Wang, Rui Wu] Northwestern Polytech Univ, Ctr Ecol & Environm Sci, Xian 710072, Peoples R China. [Cheng, Shang] Chongqing Acad Anim Sci, Chongqing 402460, Peoples R China. RP Huang, Q (reprint author), Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China.; Huang, Q; Evans, JD (reprint author), ARS, USDA, Bee Res Lab, BARC East Bldg 306, Beltsville, MD 20705 USA.; Wang, RW (reprint author), Northwestern Polytech Univ, Ctr Ecol & Environm Sci, Xian 710072, Peoples R China. EM qiang-huang@live.com; Jay.Evans@ars.usda.gov OI Evans, Jay/0000-0002-0036-4651 FU United States Department of Agriculture-National Institute of Food and Agriculture [2014-67013-21784] FX This study was supported by the United States Department of Agriculture-National Institute of Food and Agriculture- 2014-67013-21784.r We thank Dawn Lopez, Michele Hamilton, and Margaret Mulhern for technical support. We appreciate Jiajie Sun and Lingyang Xu for statistical support. The work is supported by USDA-NIFA grant 2014-67013-21784 and USDA-NIFA had no role in study design, analysis, and publication of the work. NR 43 TC 0 Z9 0 U1 4 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 3 PY 2016 VL 11 IS 2 AR e0147549 DI 10.1371/journal.pone.0147549 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9OF UT WOS:000369550200045 PM 26840596 ER PT J AU Newcombe, G Campbell, J Griffith, D Baynes, M Launchbaugh, K Pendleton, R AF Newcombe, George Campbell, Jason Griffith, David Baynes, Melissa Launchbaugh, Karen Pendleton, Rosemary TI Revisiting the Life Cycle of Dung Fungi, Including Sordaria fimicola SO PLOS ONE LA English DT Article ID COPROPHILOUS FUNGI; BROMUS-TECTORUM; ENDOPHYTES; LEAVES; FREQUENCIES; DIVERSITY AB Dung fungi, such as Sordaria fimicola, generally reproduce sexually with ascospores discharged from mammalian dung after passage through herbivores. Their life cycle is thought to be obligate to dung, and thus their ascospores in Quaternary sediments have been interpreted as evidence of past mammalian herbivore activity. Reports of dung fungi as endophytes would seem to challenge the view that they are obligate to dung. However, endophyte status is controversial because surface-sterilization protocols could fail to kill dung fungus ascospores stuck to the plant surface. Thus, we first tested the ability of representative isolates of three common genera of dung fungi to affect plant growth and fecundity given that significant effects on plant fitness could not result from ascospores merely stuck to the plant surface. Isolates of S. fimicola, Preussia sp., and Sporormiella sp. reduced growth and fecundity of two of three populations of Bromus tectorum, the host from which they had been isolated. In further work with S. fimicola we showed that inoculations of roots of B. tectorum led to some colonization of above ground tissues. The same isolate of S. fimicola reproduced sexually on inoculated host plant tissues as well as in dung after passage through sheep, thus demonstrating a facultative rather than an obligate life cycle. Finally, plants inoculated with S. fimicola were not preferred by sheep; preference had been expected if the fungus were obligate to dung. Overall, these findings make us question the assumption that these fungi are obligate to dung. C1 [Newcombe, George; Campbell, Jason; Launchbaugh, Karen] Univ Idaho, Dept Forest Rangelands & Fire Sci, Moscow, ID 83843 USA. [Griffith, David; Baynes, Melissa] Univ Idaho, Environm Sci Program, Moscow, ID 83843 USA. [Campbell, Jason] Univ Idaho, Palouse Res Educ & Extens Ctr, Moscow, ID 83843 USA. [Griffith, David] Univ Idaho, Ctr Resilient Communities, Moscow, ID 83843 USA. [Pendleton, Rosemary] US Forest Serv, Rocky Mt Res Stn, Albuquerque, NM USA. RP Newcombe, G (reprint author), Univ Idaho, Dept Forest Rangelands & Fire Sci, Moscow, ID 83843 USA. EM georgen@uidaho.edu OI Griffith, David/0000-0001-6702-6764 FU U.S. Forest Service Rocky Mountain Research Station [RMRS 10-CR-11221632-182] FX Support was provided to GN by the U.S. Forest Service Rocky Mountain Research Station in the form of grant RMRS 10-CR-11221632-182. Their website is www.fs.fed.us/rmrs. RP of the RMRS collaborated fully in all aspects of the project. NR 41 TC 0 Z9 0 U1 9 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 3 PY 2016 VL 11 IS 2 AR e0147425 DI 10.1371/journal.pone.0147425 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DC9OF UT WOS:000369550200039 PM 26839959 ER PT J AU Kim, SP Lee, SJ Nam, SH Friedmana, M AF Kim, Sung Phil Lee, Sang Jong Nam, Seok Hyun Friedmana, Mendel TI Elm Tree (Ulmus parvifolia) Bark Bioprocessed with Mycelia of Shiitake (Lentinus edodes) Mushrooms in Liquid Culture: Composition and Mechanism of Protection against Allergic Asthma in Mice SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE bioprocessing bioactive; biofunctional; proximate analysis; GC/MS; elm tree bark; Ulmus parvifolia; shiitake mushroom mycelia; Lentinus edodes; mice feeding study; allergic asthma prevention; functional food ID REGULATORY T-CELLS; CHEMICAL-COMPOSITION; EXTRACTS; POLYSACCHARIDE; BIOMARKERS; INFLAMMATION; DAVIDIANA; ASSAYS; IL-10 AB Mushrooms can break down complex plant materials into smaller, more digestible and bioactive compounds. The present study investigated the antiasthma effect of an Ulmus parvifolia bark extract bioprocessed in Lentinus edodes liquid mycelium culture (BPUBE) against allergic asthma in chicken egg ovalbumin (OVA)-sensitized/challenged mice. BPUBE suppressed total IgE release from U266B1 cells in a dose-dependent manner without cytotoxicity. Inhibitory activity of BPUBE against OVA-specific IgE secretion in bronchoalveolar lavage fluid (BALF) was observed in OVA-sensitized/challenged asthmatic mice. BPUBE also inhibited OVA-specific IgG and IgG1 secretion into serum from the allergic mice, suggesting the restoration of a Th2-biased immune reaction to a Thl/Th2-balanced status, as indicated by the Thl/Th2 as well as regulatory T cell (Treg) cytoldne profile changes caused by BPUBE in serum or BALF. Inflammatory cell counts in BALF and lung histology showed that leukocytosis and eosinophilia induced by OVA-sensitization/challenge were inhibited by the oral administration of BPUBE. Amelioration of eosinophil infiltration near the trachea was associated with reduced eotaxin and vascular cell adhesion molecule-1 (VCAM-1) levels. Changes in proinflammatory mediator levels in BALF suggest that BPUBE decreased OVA-sensitization induced elevation of leukotriene C-4 (LTC4) and prostaglandin D-2 (PGD(2)). The finding that asthma-associated biomarker levels of OVA-sensitized/challenged mice were much more inhibited with BPUBE treatment than NPUBE (not-bioprocessed Ulmus parvifolia extract) treatment suggested the production of new bioactive compounds by the mushroom mycelia that may be involved in enhancing the observed antiasthmatic properties. The possible relation of the composition determined by proximate analysis and GC/MS to observed bioactivity is discussed. The results suggest that the elm tree (Ulmus parvifolia) bark bioprocessed with mycelia of shiitake (Lentinus edodes) mushrooms has the potential to prevent and/or treat allergic asthma. C1 [Kim, Sung Phil; Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Lee, Sang Jong] STR Biotech Co Ltd, Chunchon 200160, South Korea. [Friedmana, Mendel] USDA, ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Nam, SH (reprint author), Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea.; Friedmana, M (reprint author), USDA, ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM shnam@ajou.ac.kr; mendel.friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 FU Technological Innovation R&D Program of the Small and Medium Business Administration (SMBA, Korea) [S-2014-C1477-00002]; Ajou University FX We thank the Technological Innovation R&D Program (No. S-2014-C1477-00002) of the Small and Medium Business Administration (SMBA, Korea) and the research fund of Ajou University for financial support. NR 44 TC 2 Z9 2 U1 2 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 3 PY 2016 VL 64 IS 4 BP 773 EP 784 DI 10.1021/acs.jafc.5b04972 PG 12 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DC9QX UT WOS:000369557200006 PM 26807923 ER PT J AU Sanford, B Holinka, LG O'Donnell, V Krug, PW Carlson, J Alfano, M Carrillo, C Wu, P Lowe, A Risatti, GR Gladue, DP Borca, MV AF Sanford, B. Holinka, L. G. O'Donnell, V. Krug, P. W. Carlson, J. Alfano, M. Carrillo, C. Wu, Ping Lowe, Andre Risatti, G. R. Gladue, D. P. Borca, M. V. TI Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus SO VIRUS RESEARCH LA English DT Article DE ASFV; African swine fever virus; Thymidine kinase ID HERPES-SIMPLEX-VIRUS; VIRULENCE-ASSOCIATED GENE; DEFICIENT MUTANTS; VACCINIA VIRUS; CHALLENGE; EXPRESSION; PROTECTION; PIGS; DNA; CONSTRUCTION AB African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs. There are no vaccines to control Africa swine fever (ASF). Experimental vaccines have been developed using genetically modified live attenuated ASFVs obtained by specifically deleting virus genes involved in virulence, including the thymidine kinase (TIC) gene. TIC has been shown to be involved in the virulence of several viruses, including ASFV. Here we report the construction of a recombinant virus (ASFV-G/V-Delta TK) obtained by deleting the TIC gene in a virulent strain of ASFV Georgia adapted to replicate in Vero cells (ASFV-GIVP30). ASFV-G/P-Delta TK demonstrated decreased replication both in primary swine macrophage cell cultures and in Vero cells compared with ASFV-G/VP30. In vivo, intramuscular administration of up to 106 TCID50 of ASFV-G/V-Delta TK does not result in ASF disease. However, these animals are not protected when challenged with the virulent parental Georgia strain. Published by Elsevier B.V. C1 [Sanford, B.; Holinka, L. G.; O'Donnell, V.; Krug, P. W.; Carlson, J.; Alfano, M.; Gladue, D. P.; Borca, M. V.] ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [O'Donnell, V.; Risatti, G. R.] Univ Connecticut, CANR, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA. [Carrillo, C.; Wu, Ping; Lowe, Andre] USDA, APHIS, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. RP Borca, MV (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, NAA, POB 848, Greenport, NY 11944 USA. EM manuel.borca@ars.usda.gov OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178 FU U.S. Department of Homeland Security [HSHQDC-11-X-00077, HSHQPM-12-X-00005]; ARS/USDA-University of Connecticut SCA [58-1940-1-190] FX We thank the Plum Island Animal Disease Center Animal Care Unit staff for excellent technical assistance. We wish to particularly thank Mrs. Melanie V. Prarat for editing the manuscript. This project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Numbers HSHQDC-11-X-00077 and HSHQPM-12-X-00005. We would like to thank ARS/USDA-University of Connecticut SCA# 58-1940-1-190 for partially supporting this work. NR 33 TC 0 Z9 1 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD FEB 2 PY 2016 VL 213 BP 165 EP 171 DI 10.1016/j.virusres.2015.12.002 PG 7 WC Virology SC Virology GA DG1PS UT WOS:000371840600019 PM 26656424 ER PT J AU Stewart, LR AF Stewart, Lucy R. TI Sequence diversity of wheat mosaic virus isolates SO VIRUS RESEARCH LA English DT Article DE Wheat mosaic virus (WMoV); High Plains disease; Nucleoprotein; Emaravirus ID HIGH-PLAINS-VIRUS; LEAF BLOTCH VIRUS; INFECTING WHEAT; RNA SEGMENTS; CAUSAL AGENT; GENOMIC RNA; DISEASE; IDENTIFICATION; MITE; MULTIPARTITE AB Wheat mosaic virus (WMoV), transmitted by eriophyid wheat curl mites (Aceria tosichella) is the causal agent of High Plains disease in wheat and maize. WMoV and other members of the genus Emaravirus evaded thorough molecular characterization for many years due to the experimental challenges of mite transmission and manipulating multisegmented negative sense RNA genomes. Recently, the complete genome sequence of a Nebraska isolate of WMoV revealed eight segments, plus a variant sequence of the nucleocapsid protein-encoding segment. Here, near-complete and partial consensus sequences of five more WMoV isolates are reported and compared to the Nebraska isolate: an Ohio maize isolate (GG1), a Kansas barley isolate (KS7), and three Ohio wheat isolates (H1, K1, W1). Results show two distinct groups of WMoV isolates: Ohio wheat isolate RNA segments had 84% or lower nucleotide sequence identity to the NE isolate, whereas GG1 and KS7 had 98% or higher nucleotide sequence identity to the NE isolate. Knowledge of the sequence variability of WMoV isolates is a step toward understanding virus biology, and potentially explaining observed biological variation. Published by Elsevier B.V. C1 [Stewart, Lucy R.] ARS, Corn Soybean & Wheat Qual Res Unit, USDA, 1680 Madison Ave, Wooster, OH 44691 USA. RP Stewart, LR (reprint author), ARS, Corn Soybean & Wheat Qual Res Unit, USDA, 1680 Madison Ave, Wooster, OH 44691 USA. EM Lucy.Stewart@ars.usda.gov FU Ohio Small Grains Marketing Program; USDA-ARS; Ohio State University FX Thanks to the Ohio Small Grains Marketing Program, USDA-ARS, and Ohio State University for funding the initial survey of Ohio wheat, and to Ohio wheat and corn growers for permitting surveys on their property. Thanks to the Molecular and Cellular Imaging Center (OSU) for RNA-sequencing and bioinformatics support, This project would not be possible without the expert technical assistance of Kristen Willie (USDA-ARS) for primer design and Sanger sequencing confirmation of RNA-Seq data. Special thanks also to Ellie Walsh (OSU), Jane Todd (USDA-ARS), and Dr. Margaret G. Redinbaugh (USDA-ARS) for critical reading of the manuscript. NR 23 TC 1 Z9 1 U1 2 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD FEB 2 PY 2016 VL 213 BP 299 EP 303 DI 10.1016/j.virusres.2015.11.013 PG 5 WC Virology SC Virology GA DG1PS UT WOS:000371840600036 PM 26590326 ER PT J AU Marzano, SYL Domier, LL AF Marzano, Shin-Yi Lee Domier, Leslie L. TI Novel mycoviruses discovered from metatranscriptomics survey of soybean phyllosphere phytobiomes SO VIRUS RESEARCH LA English DT Article DE High-throughput sequencing metatranscriptome; Phyllosphere; Soybean; Viromes ID DOUBLE-STRANDED-RNA; MOLECULAR CHARACTERIZATION; SCLEROTINIA-SCLEROTIORUM; BIOLOGICAL-CONTROL; CHESTNUT BLIGHT; FUNGAL VIRUSES; DNA MYCOVIRUS; PLANT; ALIGNMENT; GENOME AB Mycoviruses can be beneficial to plants in that they can debilitate pathogenic fungi thereby reducing the severity of associated plant diseases. Studies to date have focused primarily on culturable fungi that represent a fraction of natural fungal populations. The nonculturable fungi, however, can harbor diverse populations of mycoviruses that reduce plant disease or enhance resistance to abiotic stress. Metatranscriptome analysis of field-grown plant samples using high-throughput sequencing offers the possibility of unbiased detection and quantification of mycoviruses regardless of the culturability of their fungal hosts together with the complete associated microbial consortia. In this study, we describe the fungal viromes of the phyllosphere of production soybean fields in Illinois, USA by analyzing the metatranscriptomes of thousands of soybean leaf samples collected during the 2008 and 2009 growing seasons. The analyses identified 25 partial genome sequences that represented at least 22 mycovirus genomes, only one of which had been described previously. The novel mycovirus genomes showed similarity to 10 distinct lineages including the genera Alphapartitivirus, Botybirnavirus, Endornavirus, Mitovirus, Mycoflexivirus, Ourmiavirus, Totivirus, Victorivirus, family Tombusviridae, order Mononegavirales, and the recently proposed genus Gemycircularvirus. The present study adds to the wealth of mycoviruses associated with plant phytobiomes and establishes groundwork needed for further characterization of the viruses. Published by Elsevier B.V. C1 [Marzano, Shin-Yi Lee; Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Domier, Leslie L.] ARS, USDA, Urbana, IL USA. [Marzano, Shin-Yi Lee] S Dakota State Univ, Dept Plant Sci, Dept Biol & Microbiol, Brookings, SD 57007 USA. RP Domier, LL (reprint author), Univ Illinois, Dept Crop Sci, ARS, USDA, Urbana, IL 61801 USA. EM leslie.domier@ars.usda.gov FU Illinois Soybean Association; United States Department of Agriculture-Agricultural Research Service FX This work was supported by a grant from the Illinois Soybean Association and the United States Department of Agriculture-Agricultural Research Service. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA or the University of Illinois and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 71 TC 7 Z9 7 U1 5 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD FEB 2 PY 2016 VL 213 BP 332 EP 342 DI 10.1016/j.virusres.2015.11.002 PG 11 WC Virology SC Virology GA DG1PS UT WOS:000371840600040 PM 26547008 ER PT J AU Shen, GQ Ashworth, DJ Gan, J Yates, SR AF Shen, Guoqing Ashworth, Daniel J. Gan, Jay Yates, Scott R. TI Biochar Amendment to the Soil Surface Reduces Fumigant Emissions and Enhances Soil Microorganism Recovery SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID HYDROGEN-SULFIDE ADSORPTION; PYROLYSIS TEMPERATURE; AMMONIUM THIOSULFATE; ACTIVATED CARBONS; BLACK CARBON; 1,3-DICHLOROPROPENE; FIELD; CHLOROPICRIN; MECHANISMS; REDUCTION AB During soil fumigation, it is ideal to mitigate soil fumigant emissions, ensure pest control efficacy, and speed up the recovery of the soil microorganism population established postapplication. However, no current fumigant emission reduction strategy can meet all these requirements. In the present study, replicated soil columns were used to study the effect of biochar derived from rice husk (BR) and green waste (BG) applied to the soil surface on 1,3-dichloropropene (1,3-D) and chloropicrin (CP) emissions and soil gas distribution, and on microorganism population re-establishment. Relative to fumigated bare soil (no emission reduction strategy), high density polyethylene (HDPE), and ammonium thiosulfate (ATS) treatments, BR gave dramatic emission reductions for both fumigants with no obvious emission peak, whereas BG was very effective only for 1,3-D. With BR application, the concentration of fumigant in the soil gas was higher than in the bare soil and ATS treatment. After the soil column experiment, mixing the BR with the fumigated soil resulted in higher soil respiration rates than were observed for HDPE and ATS treatments. Therefore, biochar amendment to the soil surface may be an effective strategy for fumigant emission reduction and the recovery of soil microorganism populations established postapplication. C1 [Shen, Guoqing] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China. [Ashworth, Daniel J.; Gan, Jay] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Ashworth, Daniel J.; Yates, Scott R.] USDA ARS, Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. RP Ashworth, DJ (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA.; Ashworth, DJ (reprint author), USDA ARS, Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. EM daniel.ashworth@ars.usda.gov FU National Natural Science Foundation of China [21477075] FX We thank Q Zhang for her technical assistance in conducting these experiments. This work was supported by the National Natural Science Foundation of China (No. 21477075). NR 55 TC 3 Z9 3 U1 22 U2 71 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD FEB 2 PY 2016 VL 50 IS 3 BP 1182 EP 1189 DI 10.1021/acs.est.5b03958 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DC8LO UT WOS:000369471300013 PM 26726779 ER PT J AU Bradford, SA Kim, H Headd, B Torkzaban, S AF Bradford, Scott A. Kim, Hyunjung Headd, Brendan Torkzaban, Saeed TI Evaluating the Transport of Bacillus subtilis Spores as a Potential Surrogate for Cryptosporidium parvum Oocysts SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SATURATED POROUS-MEDIA; PHYSICOCHEMICAL FILTRATION; SURFACE MACROMOLECULES; SOLUTION CHEMISTRY; COLLOID RETENTION; WATER-TREATMENT; PARTICLES; ATTACHMENT; ADHESION; REMOVAL AB The U.S. Environmental Protection Agency has recommended the use of aerobic spores as an indicator for Cryptosporidium oocysts when determining groundwater under the direct influence of surface water. Surface properties, interaction energies, transport, retention, and release behavior of B. subtilis spores were measured over a range of physicochemical conditions, and compared with reported information for C. parvum oocysts. Interaction energy calculations predicted a much larger energy barrier and a shallower secondary minimum for spores than oocysts when the solution ionic strength (IS) equaled 0.1, 1, and 10 mM, and no energy barrier when the IS = 100 mM. Spores and oocysts exhibited similar trends of increasing retention with IS and decreasing Darcy water velocity (q(w)), and the predicted setback distance to achieve a six log removal was always larger for spores than oocysts. However, low levels of observed spore and oocyst release significantly influenced the predicted setback distance, especially when the fraction of reversibly retained microbes (F-rev) was high. An estimate for F-rev was obtained from large release pulses of spore and oocyst when the IS was reduced to deionized water. The value of F-rev always increased with q(w), whereas an opposition trend for F-rev with IS was observed for spores (decreasing) and oocysts (increasing). C1 [Bradford, Scott A.; Headd, Brendan] ARS, US Salin Lab, USDA, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Kim, Hyunjung] Chonbuk Natl Univ, Dept Mineral Resources & Energy Engn, 664-14 Duckjin, Jeonju 561756, Jeonbuk, South Korea. [Torkzaban, Saeed] CSIRO, Land & Water, Glen Osmond, SA 5064, Australia. RP Bradford, SA (reprint author), ARS, US Salin Lab, USDA, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Scott.Bradford@ars.usda.gov FU USDA; ARS; NP [214] FX This research was supported by the USDA, ARS, NP 214. We thank Philip Berger of the U.S. EPA for helpful comments and discussion concerning this manuscript. NR 61 TC 0 Z9 0 U1 5 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD FEB 2 PY 2016 VL 50 IS 3 BP 1295 EP 1303 DI 10.1021/acs.est.5b05296 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DC8LO UT WOS:000369471300026 PM 26720840 ER PT J AU McHugh, T AF McHugh, Tara TI How Tofu Is Processed SO FOOD TECHNOLOGY LA English DT Editorial Material C1 [McHugh, Tara] USDA ARS, Albany, CA USA. RP McHugh, T (reprint author), USDA ARS, Albany, CA USA. EM tara.mchugh@ars.usda.gov NR 4 TC 0 Z9 0 U1 0 U2 0 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD FEB PY 2016 VL 70 IS 2 BP 72 EP 74 PG 3 WC Food Science & Technology SC Food Science & Technology GA DE4BJ UT WOS:000370573900018 ER PT J AU Taha, H Wilkinson, J Bornstein, R Xiao, QF McPherson, G Simpson, J Anderson, C Lau, S Lam, J Blain, C AF Taha, Haider Wilkinson, James Bornstein, Robert Xiao, Qingfu McPherson, Greg Simpson, Jim Anderson, Charles Lau, Steven Lam, Janice Blain, Cindy TI An urban-forest control measure for ozone in the Sacramento, CA Federal Non-Attainment Area (SFNA) SO Sustainable Cities and Society LA English DT Article DE Air quality; Air quality modeling; Biogenic emissions; Biogenic volatile organic compounds; Control measure; Meteorological modeling; Ozone; State implementation plan; Urban forest ID MESOSCALE MODEL; AIR-QUALITY; PARAMETERIZATION; SCHEME; TESTS; MM5; SENSITIVITY; SIMULATION AB Urban forest strategies of gradually replacing high emitters of biogenic volatile organic compounds (BVOC) with low-emitting species are being considered as voluntary or emerging control measures for maintenance of the 8-h ozone standard in the Sacramento Federal Non-Attainment Area (SFNA). We describe a regulatory modeling study demonstrating the air-quality impacts of such measures as well as of strategies that increase net canopy cover. The results indicate that changing the mix of urban trees can improve air quality. The daily reductions in ozone resulting from species replacement alone reach up to 0.50 ppb. With a more geographically targeted replacement, the daily reductions increase to 3 ppb. Population-weighted exposure to ozone is reduced by up to 34% relative to the NAAQS (120 ppb) and 12% relative to the CAAQS (90 ppb). The 8-h average peak ozone is reduced by 2%. If, in addition to species replacement, the net canopy cover is increased, the reductions in ozone become much larger but increases in ozone also occur. In some scenarios, the air-quality impacts are 10 times as large as those of only replacing 650,000 trees (control measure). Furthermore, because of the canopy growth (including the replacement trees) relative to 2000-2005, the SFNA is cooled by up to 1.2 degrees C by 2018 and 1.6 degrees C by 2023. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Taha, Haider] Altostratus Inc, 940 Toulouse Way, Martinez, CA 94553 USA. [Wilkinson, James] Alpine Geophys, Golder Associates, Arvada, CO USA. [Bornstein, Robert] San Jose State Univ, San Jose, CA 95192 USA. [Xiao, Qingfu] Univ Calif Davis, Davis, CA 95616 USA. [McPherson, Greg; Simpson, Jim] US Forest Serv, Pacific Southwest Res Stn, Washington, DC 20250 USA. [Anderson, Charles; Lau, Steven; Lam, Janice] Sacramento Metropolitan Air Qual Management Dist, Sacramento, CA USA. [Blain, Cindy] Sacramento Tree Fdn, Calif Releaf, Sacramento, CA USA. RP Taha, H (reprint author), Altostratus Inc, 940 Toulouse Way, Martinez, CA 94553 USA. EM haider@altostratus.com FU Sacramento Metropolitan Air Quality Management District (SMAQMD) [E2007008]; Altostratus Inc. FX This paper summarizes work sponsored by the Sacramento Metropolitan Air Quality Management District (SMAQMD) under Contract E2007008 "Urban Forest for Clean Air Demonstration Project" (UFFCA) with Altostratus Inc. Findings in this paper do not necessarily reflect the views of the SMAQMD, their staff, employees, or contractors. NR 45 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2210-6707 EI 2210-6715 J9 SUSTAIN CITIES SOC JI Sust. Cities Soc. PD FEB PY 2016 VL 21 BP 51 EP 65 DI 10.1016/j.scs.2015.11.004 PG 15 WC Construction & Building Technology; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Construction & Building Technology; Science & Technology - Other Topics; Energy & Fuels GA EE1CH UT WOS:000389317900005 ER PT J AU Lager, KM Kulshreshtha, V AF Lager, Kelly M. Kulshreshtha, Vikas TI Development and application of the effective vaccine and other strategies against recent porcine epidemic diarrhea outbreaks SO JAPANESE JOURNAL OF VETERINARY RESEARCH LA English DT Article AB In April 2013 porcine epidemic diarrhea virus (PEDV) was detected for the first time in the United States and within months the virus was detected throughout all major swine producing regions of the US. The virus caused severe diarrhea in young piglets leading to high mortality. Within the first year of the epidemic about 50% of the sows in the US became infected resulting in a 7-8% loss in total pig production. Interestingly, the US PEDV isolates were almost identical to contemporary Chinese PEDV isolates. The initial control measures for PEDV in the US included a controlled oral exposure of the sow herd to infectious virus, and an increased cleaning and sanitation of transport vehicles and facilities. Controlled exposure or "feedback" involved inoculating the entire sow herd with infectious material (feces collected from sick and infected piglets) in an attempt to stimulate active immunity against PEDV in the sow herd. The goal was to develop immunity in the sow and transfer that immunity to pigs at birth through colostrum. In most acutely affected herds this practice would take several weeks to induce a protective immunity in sows that would provide clinical protection to newborn piglets. The duration of this sow immunity is not known. In 2014, two PEDV vaccines became commercially available. One was an inactivated whole virus vaccine; the second vaccine was a non-replicating viral vector that expresses a portion of the PEDV spike gene. To date there is limited experimental data available on the efficacy of either vaccine. However, both have been used extensively in the field to vaccinate sows and there are anecdotal reports supporting the use of the vaccines to reduce PEDV losses. During the second year of the epidemic the incidence of new cases declined dramatically although there was a small, but steady reporting of new cases indicating horizontal spread of the virus among swine farms continued, but at a much reduced rate. It is not clear why this occurred, but the reduced spread of the virus to naive herds may be related to intense sanitation efforts of transport vehicles and restrictions on movement of people and fomites among farms. During the second, and now third year of the emergence of PEDV in the US, there are reports of re-breaks in previously infected herds, but these events are less severe than the original disease break. It is not clear if the re-breaks represent a pocket of naive animals (replacement sows), or waning of immunity in the sows. In most swine herds there is a high replacement rate for sows where frequently 50% of the sow herd is replaced every year. It would be expected that these herds would have an unstable herd immunity to many endemic swine diseases including PEDV. Current strategies to control PEDV still involve "feedback" to inoculate replacement gilts, increased biosecurity practices that include extensive cleaning of barns, transport vehicles, restrictions on movement of people, and frequent testing of feed additives in an effort to reduce the threat of introducing new PEDV isolates to a farm through the purchase of feed. Commercial vaccines are still being used although their use may be reduced in an effort to save money in herds that do not currently have clinical disease. At the time of this writing, pork producers in the US are hoping PEDV will continue to fade away, however, winter is considered to be the best season for the virus and there is great concern that with the high replacement rate for sows, PEDV may become a significant problem again during the winter of 2015/2016. C1 [Lager, Kelly M.; Kulshreshtha, Vikas] USDA ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. RP Lager, KM (reprint author), USDA ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. EM kelly.lager@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU HOKKAIDO UNIV PI SAPPORO PA ED COMMITTEE JAPANESE JOURNAL VETERINARY RES, GRAD SCH VET MED, KITA 18 NISHI 9 KITAKU, SAPPORO, 060-0818, JAPAN SN 0047-1917 J9 JPN J VET RES JI Jpn. J. Vet. Res. PD FEB PY 2016 VL 64 SU 1 BP S39 EP + DI 10.14943/jjvr.64.suppl.s39 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DY0HJ UT WOS:000384777600006 ER PT J AU Stice, CP Hussain, S Liu, C Ausman, LM Wang, XD Greenberg, AS AF Stice, Camilla P. Hussain, Sajid Liu, Chun Ausman, Lynne M. Wang, Xiang-Dong Greenberg, Andrew S. TI Deletion of tumor progression locus 2 attenuates alcohol-induced hepatic inflammation SO HEPATOBILIARY SURGERY AND NUTRITION LA English DT Article DE Alcohol; inflammation; liver disease; tumor progression locus 2 (TPL2) ID INDUCED LIVER-INJURY; HEPATOCELLULAR-CARCINOMA; TRANSCRIPTION FACTORS; MOLECULAR-MECHANISMS; SIGNALING PATHWAYS; FATTY LIVER; FED RATS; DISEASE; KINASE; CELLS AB Background: The pathogenesis of alcoholic liver disease (ALD) involves the interaction of several inflammatory signaling pathways. Tumor progression locus 2 (TPL2), also known as Cancer Osaka Thyroid (COT) and MAP3K8, is a serine-threonine kinase that functions as a critical regulator of inflammatory pathways by up-regulating production of inflammatory cytokines. The present study aims to fill the gap in knowledge regarding the involvement of TPL2 in the mechanism of alcohol-induced hepatic inflammation. Methods: Male TPL2(-/-) knockout (TPL2KO) mice and TPL2(+/+) wild-type (WT) mice were group pair-fed with Lieber-DeCarli liquid ethanol diet (EtOH diet, 27% energy from EtOH) or control diet (ctrl diet) for 4 weeks. Both histological and molecular biomarkers involved in the induction of hepatic inflammation by alcohol consumption were examined. Results: Consumption of the EtOH diet in WT mice lead to a significant induction of TPL2 mRNA expression as compared with WT mice fed ctrl diet. A significant induction in inflammatory foci and steatosis was also observed in WT mice fed EtOH diet. The deletion of TPL2 significantly reduced inflammatory foci in the liver of mice consuming both ctrl and EtOH diets as compared to their respective WT controls. This reduction was associated with suppression of hepatic inflammatory gene expression of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) and macrophage marker F4/80. In addition, histological analysis of livers revealed that TPL2 deletion resulted in reduced steatosis in both ctrl (significant) and EtOH (non-significant) diet-fed mice as compared to their respective WT controls. Conclusions: The demonstration that TPL2 deletion attenuates alcohol-induced hepatic inflammation provides evidence of a novel role for TPL2 in the pathogenesis of ALD. C1 [Stice, Camilla P.; Liu, Chun; Ausman, Lynne M.; Wang, Xiang-Dong] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Boston, MA USA. [Hussain, Sajid; Greenberg, Andrew S.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Obes & Metab Lab, Boston, MA USA. RP Wang, XD; Greenberg, AS (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu; andrew.greenberg@tufts.edu FU NIDDK NIH HHS [T32 DK062032, R01 DK098606, P30 DK046200] NR 29 TC 0 Z9 0 U1 1 U2 1 PU AME PUBL CO PI SHEUNG WAN PA ROOM 604 6-F HOLLYWOOD CENTER, 77-91, QUEENS ROAD, SHEUNG WAN, HONG KONG 00000, PEOPLES R CHINA SN 2304-3881 EI 2304-389X J9 HEPATOL SURG NUTR JI HEPATOL. SURG. NUTR. PD FEB PY 2016 VL 5 IS 1 BP 29 EP 37 DI 10.3978/j.issn.2304-3881.2015.12.11 PN 2 PG 9 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA DT8KW UT WOS:000381740100003 PM 26904554 ER PT J AU Chimezie, C Ewing, A Schexnayder, C Bratton, M Glotser, E Skripnikova, E Sa, P Boue, S Stratford, RE AF Chimezie, Chukwuemezie Ewing, Adina Schexnayder, Chandler Bratton, Melyssa Glotser, Elena Skripnikova, Elena Sa, Pedro Boue, Stephen Stratford, Robert E., Jr. TI Glyceollin Effects on MRP2 and BCRP in Caco-2 Cells, and Implications for Metabolic and Transport Interactions SO JOURNAL OF PHARMACEUTICAL SCIENCES LA English DT Article DE active transport; drug interaction; efflux pumps; food effects; flavonoids; genistein; glyceollin; intestinal absorption; intestinal metabolism; phytoestrogen ID RESISTANCE PROTEIN BCRP; MULTIDRUG-RESISTANCE; P-GLYCOPROTEIN; BREAST; FLAVONOIDS; SOY; GENISTEIN; MODEL; DISPOSITION; MODULATION AB Glyceollins are phytoalexins produced in soybeans under stressful growth conditions. On the basis of prior evaluations, they show potential to treat multiple diseases, including certain cancers, Type 2 diabetes, and cardiovascular conditions. The aim of the present study was to expand on recent studies designed to initially characterize the intestinal disposition of glyceollins. Specifically, studies were undertaken in Caco-2 cells to evaluate glyceollins' effects on apical efflux transporters, namely, MRP2 and BCRP, which are the locus of several intestinal drug-drug and drug-food interactions. 5- (and 6)-carboxy2', 7'-dichloroflourescein (CDF) was used to provide a readout on MRP2 activity, whereas BODIPY-prazosin provided an indication of BCRP alteration. Glyceollins were shown to reverse MRP2-mediated CDF transport asymmetry in a concentration-dependent manner, with activity similar to the MRP2 inhibitor, MK-571. Likewise, they demonstrated concentration-dependent inhibition of BCRP-mediated efflux of BODIPY-prazosin with a potency similar to that of Ko143. Glyceollin did not appreciably alter MRP2 or BCRP expression following 24 h of continuous exposure. The possibility that glyceollin mediated inhibition of genistein metabolite efflux by either transporter was evaluated. However, results demonstrated an interaction at the level of glyceollin inhibition of genistein metabolism rather than inhibition of metabolite transport. (C) 2016 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved. C1 [Chimezie, Chukwuemezie; Ewing, Adina; Schexnayder, Chandler; Bratton, Melyssa; Glotser, Elena; Skripnikova, Elena; Stratford, Robert E., Jr.] Xavier Univ Louisiana, Dept Basic Pharmaceut Sci, Coll Pharm, New Orleans, LA 70125 USA. [Sa, Pedro] Univ Fed Vale Sao Francisco, BR-56403917 Petrolina, PE, Brazil. [Boue, Stephen] USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Stratford, Robert E., Jr.] Duquesne Univ, Grad Sch Pharmaceut Sci, Mylan Sch Pharm, Room 425 Mellon Hall,600 Forbes Ave, Pittsburgh, PA 15282 USA. RP Stratford, RE (reprint author), Xavier Univ Louisiana, Dept Basic Pharmaceut Sci, Coll Pharm, New Orleans, LA 70125 USA. EM stratfordr@duq.edu FU Louisiana Cancer Research Consortium Seed Grant [OSP-7011-003]; National Institutes on Minority Health and Health Disparities (NIMHD), National Institutes of Health (NIH), Department of Health and Human Services (DHHS) [2G12MD007595] FX This work was funded by a Louisiana Cancer Research Consortium Seed Grant (OSP-7011-003) and in part by Grant Number 2G12MD007595 from the National Institutes on Minority Health and Health Disparities (NIMHD), National Institutes of Health (NIH), Department of Health and Human Services (DHHS), and its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIMHD or NIH. NR 33 TC 1 Z9 1 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-3549 EI 1520-6017 J9 J PHARM SCI-US JI J. Pharm. Sci. PD FEB PY 2016 VL 105 IS 2 BP 972 EP 981 DI 10.1002/jps.24605 PG 10 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA DT8TQ UT WOS:000381768500065 PM 26296158 ER PT J AU Dasuri, K Zhang, L Kim, SOKF Bruce-Keller, AJ Keller, JN AF Dasuri, Kalavathi Zhang, Le Kim, Sun O. K. Fernandez Bruce-Keller, Annadora J. Keller, Jeffrey N. TI Dietary and donepezil modulation of mTOR signaling and neuroinflammation in the brain SO BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE LA English DT Article DE Brain; Aging; High fat diet; Obesity; Neurodegenerative disorders; mTOR pathway; Donepezil; Neuroinflammation ID HIGH-FAT-DIET; INDUCED INSULIN-RESISTANCE; TAUOPATHY MOUSE MODEL; ALZHEIMERS-DISEASE; MAMMALIAN TARGET; ADIPOSE-TISSUE; GLUTAMATE NEUROTOXICITY; COGNITIVE IMPAIRMENT; METABOLIC SYNDROME; OXIDATIVE STRESS AB Recent clinical and laboratory evidences suggest that high fat diet (HFD) induced obesity and its associated metabolic syndrome conditions promotes neuropathology in aging and age-related neurological disorders. However, the effects of high fat diet on brain pathology are poorly understood, and the effective strategies to overcome these effects remain elusive. In the current study, we examined the effects of HFD on brain pathology and further evaluated whether donepezil, an AChE inhibitor with neuroprotective functions, could suppress the ongoing HFD induced pathological changes in the brain. Our data demonstrates that HFD induced obesity results in increased neuroinflammation and increased AChE activity in the brain when compared with the mice fed on low fat diet (LFD). HFD administration to mice activated mTOR pathway resulting in increased phosphorylation of mTORser2448, AKTthr3 8 and S6K proteins involved in the signaling. Interestingly, donepezil administration with HFD suppressed HFD induced increases in AChE activity, and partially reversed HFD effects on microglial reactivity and the levels of mTOR signaling proteins in the brain when compared to the mice on LFD alone. However, gross levels of synaptic proteins were not altered in the brain tissues of mice fed either diet with or without donepezil. In conclusion, these results present a new insight into the detrimental effects of HFD on brain via microglial activation and involvement of mTOR pathway, and further demonstrates the possible therapeutic role for donepezil in ameliorating the early effects of HFD that could help preserve the brain function in metabolic syndrome conditions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dasuri, Kalavathi; Zhang, Le; Kim, Sun O. K. Fernandez; Bruce-Keller, Annadora J.; Keller, Jeffrey N.] LSU Syst, Pennington Biomed Res Ctr, Baton Rouge, LA 70810 USA. [Dasuri, Kalavathi] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Zhang, Le] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Geriatr, Inst Aging, Wuhan 430074, Peoples R China. RP Dasuri, K (reprint author), LSU Syst, Pennington Biomed Res Ctr, Baton Rouge, LA 70810 USA.; Dasuri, K (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM Kalavathidasuri@gmail.com FU COBRE from the National Institutes of Health [NIH 2P20-RR021945]; NORC from the National Institutes of Health [NIH 2P30-DK072476]; Pennington Animal Metabolism and Behavior Core; NIH; Hibernia National Bank/Edward G Schleider Chair FX This work used the facilities of the Cell Biology and Bioimaging Core which are supported in part by COBRE (NIH 2P20-RR021945) and NORC (NIH 2P30-DK072476) center grants from the National Institutes of Health, the Pennington Animal Metabolism and Behavior Core. The work was supported by funds from the NIH and Hibernia National Bank/Edward G Schleider Chair to JNK. NR 57 TC 1 Z9 1 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-4439 EI 0006-3002 J9 BBA-MOL BASIS DIS JI Biochim. Biophys. Acta-Mol. Basis Dis. PD FEB PY 2016 VL 1862 IS 2 BP 274 EP 283 DI 10.1016/j.bbadis.2015.11.002 PG 10 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA DN9SH UT WOS:000377420500014 PM 26554604 ER PT J AU Telkanranta, H Marchant-Forde, JN Valros, A AF Telkanranta, H. Marchant-Forde, J. N. Valros, A. TI Tear staining in pigs: a potential tool for welfare assessment on commercial farms SO ANIMAL LA English DT Article DE pig; tear staining; tail biting; ear biting; enrichment ID HARDERIAN-GLAND; EXPLORATORY-BEHAVIOR; LABORATORY RATS; TAIL; STRESS; ENRICHMENT; PORPHYRIN AB Tear staining or chromodacryorrhea refers to a dark stain below the inner corner of the eye, caused by porphyrin-pigmented secretion from the Harderian gland. It has been shown to be a consistent indicator of stress in rats and to correlate with social stress and a barren environment in pigs. The current study was, to our knowledge, the first to test it on commercial pig farms as a potential welfare indicator. The study was carried out on three commercial farms in Finland, in connection to a larger study on the effects of different types of manipulable objects on tail and ear biting and other behavioural parameters. Farm A was a fattening farm, on which 768 growing-finishing pigs were studied in 73 pens. Farm B had a fattening unit, in which 656 growing-finishing pigs were studied in 44 pens, and a farrowing unit, in which 29 sows and their litters totalling 303 piglets were studied in 29 pens. Farm C was a piglet-producing farm, on which 167 breeder gilts were studied in 24 pens. Data collection included individual-level scoring of tear staining; scoring of tail and ear damage in the growing-finishing pigs and breeder gilts; a novel object test for the piglets; and a novel person test for the growing-finishing pigs on Farm B and the breeder gilts on Farm C. On Farm A, tear staining was found to correlate with tail damage scores (n = 768, r(s) = 0.14, P < 0.001) and ear damage scores (n = 768, r(s) = 0.16, P < 0.001). In the growing-finishing pigs on Farm B, tear staining of the left eye correlated with tail damage (n = 656, r(s) = 0.12, P < 0.01) and that of the right eye correlated with ear damage (n = 656, r(s) = 0.10, P < 0.01). On Farm A, tear-staining sores were lower in the treatment with three different types of manipulable objects as compared with controls (mean scores 3.3 and 19, respectively, n = 31, F-29 = 42, P < 0.05). In the suckling piglets on Farm B, tear staining correlated with the latency to approach a novel object (n = 29, r(p) = 0.41, P < 0.05). Although correlations with tail and ear damage were low, it was concluded that tear staining has promising potential as a new, additional welfare indicator for commercial pig farming. Further research is needed on the mechanisms of tear staining. C1 [Telkanranta, H.; Valros, A.] Univ Helsinki, Fac Vet Med, Dept Prod Anim Med, POB 57, FIN-00014 Helsinki, Finland. [Marchant-Forde, J. N.] ARS, USDA, LBRU, 1255 Russell St, W Lafayette, IN 47907 USA. RP Telkanranta, H (reprint author), Univ Helsinki, Fac Vet Med, Dept Prod Anim Med, POB 57, FIN-00014 Helsinki, Finland. EM helena.telkanranta@helsinki.ki FU Ministry of Agriculture and Forestry in Finland FX The study was funded by the Ministry of Agriculture and Forestry in Finland. The authors would also like to thank Tuulia Enbom, Sara Holms and Marika Hakonen for assistance with the on-farm scoring of tear staining and tail and ear damage, Antti Flyckt for technical assistance with the video recordings, Gudrun Illmann and Angela Wright for fruitful discussions during the writing of the manuscript, the pig farmers and their staff for cooperation, and the two reviewers for their fruitful comments on the manuscript. NR 26 TC 0 Z9 0 U1 7 U2 14 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD FEB PY 2016 VL 10 IS 2 BP 318 EP 325 DI 10.1017/5175173111500172X PG 8 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA DN5PC UT WOS:000377121500016 PM 26303891 ER PT J AU Jennings, V Larson, L Yun, J AF Jennings, Viniece Larson, Lincoln Yun, Jessica TI Advancing Sustainability through Urban Green Space: Cultural Ecosystem Services, Equity, and Social Determinants of Health SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article ID PHYSICAL-ACTIVITY; ENVIRONMENTAL-HEALTH; NEIGHBORHOOD GREEN; PROPERTY-VALUES; PUBLIC-HEALTH; MENTAL-HEALTH; NEARBY NATURE; INNER-CITY; PARKS; COMMUNITY C1 [Jennings, Viniece] US Forest Serv, Southern Res Stn, Integrating Human & Nat Syst, USDA, 320 Green St, Athens, GA 30602 USA. [Larson, Lincoln] Clemson Univ, Dept Pk Recreat & Tourism Management, Clemson, SC 29634 USA. [Yun, Jessica] Georgetown Univ, Dept Sci Technol & Int Affairs, Washington, DC 20057 USA. RP Jennings, V (reprint author), US Forest Serv, Southern Res Stn, Integrating Human & Nat Syst, USDA, 320 Green St, Athens, GA 30602 USA. EM vjennings02@fs.fed.us; lrl@clemson.edu; jjy24@georgetown.edu OI Larson, Lincoln/0000-0001-9591-1269 FU USDA Forest Service, Southern Research Station FX This project was funded by a joint venture agreement with the USDA Forest Service, Southern Research Station. Any conclusions or recommendations in this article are those of the authors and do not necessarily reflect the views of the federal government. NR 146 TC 7 Z9 7 U1 35 U2 47 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD FEB PY 2016 VL 13 IS 2 AR 196 DI 10.3390/ijerph13020196 PG 15 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DG2ME UT WOS:000371900500030 PM 26861365 ER PT J AU Schwarz, K Pouyat, RV Yesilonis, I AF Schwarz, Kirsten Pouyat, Richard V. Yesilonis, Ian TI Legacies of Lead in Charm City's Soil: Lessons from the Baltimore Ecosystem Study SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article ID WORLDWIDE REGULATORY GUIDANCE; RESIDENTIAL SOIL; URBAN SOILS; HEAVY-METALS; SPATIAL-DISTRIBUTION; CHILDRENS BLOOD; CONTAMINATION; EXPOSURE; CADMIUM; USA C1 [Schwarz, Kirsten] No Kentucky Univ, Dept Biol Sci, Nunn Dr, Highland Hts, KY 41099 USA. [Pouyat, Richard V.] USDA, Forest Serv, Res & Dev, Washington, DC 20250 USA. [Yesilonis, Ian] USDA, Forest Serv, 5523 Res Pk,Suite 350, Baltimore, MD 21228 USA. RP Schwarz, K (reprint author), No Kentucky Univ, Dept Biol Sci, Nunn Dr, Highland Hts, KY 41099 USA. EM schwarzk1@nku.edu; rpouyat@fs.fed.us; iyesilonis@fs.fed.us FU National Science Foundation [DEB 1027188]; University of Maryland Baltimore County, Center for Urban Environmental Research and Education grant from the Environmental Protection Agency [R-82818204] FX USDA Forest Service's Northern Research Station; Baltimore Ecosystem Study Long Term Ecological Research grant from the National Science Foundation (Grant No. DEB 1027188); and University of Maryland Baltimore County, Center for Urban Environmental Research and Education grant from the Environmental Protection Agency (R-82818204) are acknowledged. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture or the Forest Service of any product to the exclusion of others that may be suitable. NR 54 TC 2 Z9 2 U1 2 U2 7 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD FEB PY 2016 VL 13 IS 2 AR 209 DI 10.3390/ijerph13020209 PG 14 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DG2ME UT WOS:000371900500062 PM 26861371 ER PT J AU Poelchau, MF Coates, BS Childers, CP de Leon, AAP Evans, JD Hackett, K Shoemaker, D AF Poelchau, Monica F. Coates, Brad S. Childers, Christopher P. de Leon, Adalberto A. Perez Evans, Jay D. Hackett, Kevin Shoemaker, DeWayne TI Agricultural applications of insect ecological genomics SO CURRENT OPINION IN INSECT SCIENCE LA English DT Article ID RHIPICEPHALUS BOOPHILUS MICROPLUS; FIELD-EVOLVED RESISTANCE; HORIZONTAL GENE-TRANSFER; TERMITE RETICULITERMES FLAVIPES; POTENTIAL ECONOMIC-IMPACT; DOUBLE-STRANDED-RNA; CATTLE TICK; TETRANYCHUS-URTICAE; LUCILIA-CUPRINA; HONEY-BEE AB Agricultural entomology is poised to benefit from the application of ecological genomics, particularly the fields of biofuels generation and pest control. Metagenomic methods can characterize microbial communities of termites, woodboring beetles and livestock pests, and transcriptomic approaches reveal molecular bases behind wood-digesting capabilities of these insects, leading to potential mechanisms for biofuel generation. Genome sequences are being exploited to develop new pest control methods, identify candidate antigens to vaccinate livestock, and discover RNAi target sequences and potential non-target effects in other insects. Gene content analyses of pest genome sequences and their endosymbionts suggest metabolic interdependencies between organisms, exposing potential gene targets for insect control. Finally, genome-wide association studies and genotyping by high-throughput sequencing promise to improve management of pesticide resistance. C1 [Poelchau, Monica F.; Childers, Christopher P.] USDA ARS, Natl Agr Lib, Beltsville, MD 20705 USA. [Coates, Brad S.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [de Leon, Adalberto A. Perez] Vet Pest Genom Ctr, Kerrville, TX 78028 USA. [Evans, Jay D.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Hackett, Kevin] USDA ARS, Off Natl Programs, Crop Prod & Protect, Beltsville, MD 20705 USA. [Shoemaker, DeWayne] USDA ARS, Imported Fire Ant & Household Insects Res Unit, Gainesville, FL 32608 USA. RP Poelchau, MF (reprint author), USDA ARS, Natl Agr Lib, Beltsville, MD 20705 USA.; Shoemaker, D (reprint author), USDA ARS, Imported Fire Ant & Household Insects Res Unit, Gainesville, FL 32608 USA. EM Monica.poelchau@ars.usda.gov; dewayne.shoemaker@ars.usda.gov OI Evans, Jay/0000-0002-0036-4651; Childers, Chris/0000-0002-1253-5550 NR 122 TC 3 Z9 3 U1 14 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2214-5745 EI 2214-5753 J9 CURR OPIN INSECT SCI JI Curr. Opin. Insect Sci. PD FEB PY 2016 VL 13 BP 61 EP 69 DI 10.1016/j.cois.2015.12.002 PG 9 WC Biology; Ecology; Entomology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Entomology GA DM4FL UT WOS:000376301800010 PM 27436554 ER PT J AU Timm, A Hallerman, E Dolloff, CA Hudy, M Kolka, R AF Timm, Anne Hallerman, Eric Dolloff, C. Andrew Hudy, Mark Kolka, Randall TI Identification of a barrier height threshold where brook trout population genetic diversity, differentiation, and relatedness are affected SO ENVIRONMENTAL BIOLOGY OF FISHES LA English DT Article DE Barrier; Brook Trout; Genetic differentiation; Individual assignment; Relatedness ID MICROSATELLITE DNA MARKERS; SALMO-TRUTTA L.; HABITAT FRAGMENTATION; SALVELINUS-FONTINALIS; CUTTHROAT TROUT; F-STATISTICS; BULL TROUT; RIVER; SOFTWARE; CHARR AB The overall goal of the study was to evaluate effects of landscape features, barriers, on Brook Trout Salvelinus fontinalis population genetics and to identify a potential barrier height threshold where genetic diversity was reduced upstream of the barrier and differentiation and relatedness increase. We screened variation at eight microsatellite DNA loci within Brook Trout populations upstream and downstream of ten putative natural barriers ranging in height from 1.5 to 61 m to quantify allelic variation, differentiation (F-ST), individual assignment probability (Q), and relatedness (r(xy)). Average gene diversity per locus (H), differentiation (F-ST), and mean relatedness values (r(xy)) became significantly greater in relation to barrier height starting at 4 m according to piecewise linear regression. This potential barrier height threshold is greater than the barrier height criterion identified for Brook Trout based on physical criteria and jumping ability (0.74 m). The 4 m barrier-height criteria can be used to identify barrier sites where Brook Trout populations may be at risk due to reduced genetic diversity and increased relatedness. C1 [Timm, Anne] USDA Forest Serv, Northern Res Stn, 5523 Res Pk Dr,Suite 350, Baltimore, MD 21228 USA. [Hallerman, Eric] Virginia Tech Univ, Dept Fish & Wildlife Conservat, 100 Cheatham Hall, Blacksburg, VA 24061 USA. [Dolloff, C. Andrew] USDA Forest Serv, Southern Res Stn, 1710 Res Ctr Dr, Blacksburg, VA 24060 USA. [Hudy, Mark] US Geol Survey, Ecosyst, 12201 Sunrise Valley Dr,Mail Stop 301, Reston, VA 20192 USA. [Kolka, Randall] USDA Forest Serv, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. RP Timm, A (reprint author), USDA Forest Serv, Northern Res Stn, 5523 Res Pk Dr,Suite 350, Baltimore, MD 21228 USA. EM altimm@fs.fed.us FU USDA Forest Service (Northern Research Station, Southern Research Station, and Region 9); Virginia Tech Department of Fish and Wildlife Conservation FX This study was funded by the USDA Forest Service (Northern Research Station, Southern Research Station, and Region 9) and the Virginia Tech Department of Fish and Wildlife Conservation. We thank K. Nislow for his feedback on this study and help with data analysis. We thank the U.S. Geological Survey, Conte Anadromous Fish Laboratory for providing laboratory facilities for processing fin clip samples. We thank B. Letcher, J. Coombs, P. Schueller, and G. Mendez for their help in the fish genetics laboratory. We thank J. Kuykendall and R. Stidham for logistical support. We also thank B. Flynn, C. B. Fox, A. Palmeri, J. Emmel, M. O'Melia; D. Belkoski, D. Hagedorn, J. Herrala, P. Lung, A. May, and M. Vincie for their hard work in the field. All procedures performed as a part of this research were performed in accordance with ethical standards approved by the Virginia Tech Animal Care and Use Committee. NR 58 TC 0 Z9 0 U1 4 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0378-1909 EI 1573-5133 J9 ENVIRON BIOL FISH JI Environ. Biol. Fishes PD FEB PY 2016 VL 99 IS 2-3 BP 195 EP 208 DI 10.1007/s10641-015-0467-4 PG 14 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DK1KM UT WOS:000374671000004 ER PT J AU Keele, JW Kuehn, LA McDaneld, TG Tait, RG Jones, SA Keel, BN Snelling, WM AF Keele, J. W. Kuehn, L. A. McDaneld, T. G. Tait, R. G. Jones, S. A. Keel, B. N. Snelling, W. M. TI Genomewide association study of liver abscess in beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE acidosis; beef cattle; genomewide association study; liver abscess; rumenitis; subacute ruminal acidosis ID LUNG LESIONS; ABNORMALITIES; EXPRESSION; SLAUGHTER; ACIDOSIS; KEGG AB Fourteen percent of U.S. cattle slaughtered in 2011 had liver abscesses, resulting in reduced carcass weight, quality, and value. Liver abscesses can result from a common bacterial cause, Fusobacterium necrophorum, which inhabits rumen lesions caused by acidosis and subsequently escapes into the blood stream, is filtered by the liver, and causes abscesses in the liver. Our aim was to identify SNP associated with liver abscesses in beef cattle. We used lung samples as a DNA source because they have low economic value, they have abundant DNA, and we had unrestricted access to sample them. We collected 2,304 lung samples from a beef processing plant: 1,152 from animals with liver abscess and 1,152 from animals without liver abscess. Lung tissue from pairs of animals, 1 with abscesses and another without, were collected from near one another on the viscera table to ensure that pairs of phenotypically extreme animals came from the same lot. Within each phenotype (abscess or no abscess), cattle were pooled by slaughter sequence into 12 pools of 96 cattle for each phenotype for a total of 24 pools. The pools were constructed by equal volume of frozen lung tissue from each animal. The DNA needed to allelotype each pool was then extracted from pooled lung tissue and the BovineHD Bead Array (777,962 SNP) was run on all 24 pools. Total intensity (TI), an indicator of copy number variants, was the sum of intensities from red and green dyes. Pooling allele frequency (PAF) was red dye intensity divided TI. Total intensity and PAF were weighted by the inverse of their respective genomic covariance matrices computed over all SNP across the genome. A false discovery rate <= 5% was achieved for 15 SNP for PAF and 20 SNP for TI. Genes within 50 kbp from significant SNP were in diverse pathways including maintenance of pH homeostasis in the gastrointestinal tract, maintain immune defenses in the liver, migration of leukocytes from the blood into infected tissues, transport of glutamine into the kidney in response to acidosis to facilitate production of bicarbonate to increase pH, aggregate platelets to liver injury to facilitate liver repair, and facilitate axon guidance. Evidence from the 35 detected SNP associations combined with evidence of polygenic variation indicate that there is adequate genetic variation in incidence rate of liver abscesses, which could be exploited to select sires for reduced susceptibility to subacute acidosis and associated liver abscess. C1 [Keele, J. W.; Kuehn, L. A.; McDaneld, T. G.; Tait, R. G.; Jones, S. A.; Keel, B. N.; Snelling, W. M.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Keele, JW (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM john.keele@ars.usda.gov NR 29 TC 1 Z9 1 U1 2 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 IS 2 BP 490 EP 499 DI 10.2527/jas2015-9887 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RA UT WOS:000374689900004 PM 27065119 ER PT J AU Littlejohn, BP Price, DM Banta, JP Lewis, AW Neuendorff, DA Carroll, JA Vann, RC Welsh, TH Randel, RD AF Littlejohn, B. P. Price, D. M. Banta, J. P. Lewis, A. W. Neuendorff, D. A. Carroll, J. A. Vann, R. C. Welsh, T. H., Jr. Randel, R. D. TI Prenatal transportation stress alters temperament and serum cortisol concentrations in suckling Brahman calves SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE calves; cortisol; prenatal stress; temperament ID PITUITARY-ADRENAL AXIS; ANIMAL TEMPERAMENT; GLUCOCORTICOID EXPOSURE; HEREFORD CATTLE; TECHNICAL NOTE; EXIT VELOCITY; BOS-TAURUS; IN-UTERO; BEHAVIOR; RESPONSES AB This experiment examined the relationship between prenatal stress and subsequent calf temperament through weaning. The prenatal stressor used was repeated transportation of pregnant Brahman cows for 2 h at 60 +/- 5, 80 +/- 5, 100 +/- 5, 120 +/- 5, and 140 +/- 5 d of gestation. Prenatally stressed calves (n = 41) were compared with controls (n = 44; dams did not undergo transportation during pregnancy) from 2 wk of age until weaning (average age at weaning = 174.8 +/- 1.3 d). Temperament was defined by pen score (PS; 1 = calm and 5 = excitable), exit velocity (EV; m/sec), and temperament score (TS; (PS + EV)/2) and was recorded for each calf on d -168, -140, -112, -84, -56, -28, and 0 relative to weaning (d 0 = weaning). Cortisol concentrations were determined in serum samples obtained on d -168, -140, -28, and 0 relative to weaning. Birth weight and weaning weight were not different between treatment groups (P > 0.1). Pen score was greater (P = 0.03) in prenatally stressed calves (2.84 +/- 0.21) relative to controls (2.31 +/- 0.21). Exit velocity was greater (P < 0.01) in prenatally stressed calves (2.1 +/- 0.14 m/sec) than in controls (1.61 +/- 0.14 m/sec). Exit velocity was affected by a treatment x calf sex interaction (P = 0.04) and was greater in prenatally stressed females. Exit velocity was also affected by day (P < 0.0001). Temperament score was greater (P = 0.01) in prenatally stressed calves (2.45 +/- 0.16) than in controls (1.95 +/- 0.16). Temperament score was affected by day (P < 0.01). Basal cortisol concentrations were greater (P = 0.04) in prenatally stressed calves (15.87 +/- 1.04 ng/mL) than in controls (13.42 +/- 1.03 ng/mL). Basal cortisol concentrations were greater (P < 0.01) in females (16.61 +/- 1.06 ng/mL) than in males (12.68 +/- 1.02 ng/mL). Cortisol concentrations were positively correlated (P < 0.01) with PS (r = 0.55, P < 0.01), EV (r = 0.4, P < 0.01), and TS (r = 0.55, P < 0.01). Overall, suckling Brahman calves that were prenatally stressed were more temperamental and had greater circulating serum concentrations of cortisol than control calves. C1 [Littlejohn, B. P.; Price, D. M.; Banta, J. P.; Lewis, A. W.; Neuendorff, D. A.; Randel, R. D.] Texas A&M Univ Syst, Texas A&M AgriLife Res, Overton, TX 75684 USA. [Littlejohn, B. P.; Price, D. M.; Welsh, T. H., Jr.] Texas A&M Univ, Texas A&M AgriLife Res, College Stn, TX 77843 USA. [Littlejohn, B. P.; Price, D. M.; Welsh, T. H., Jr.] Texas A&M Univ, Coll Stn, Dept Anim Sci, College Stn, TX 77843 USA. [Carroll, J. A.] ARS, USDA, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Vann, R. C.] Mississippi State Univ, Mississippi Agr & Forestry Expt Stn, Raymond, MS 39154 USA. RP Randel, RD (reprint author), Texas A&M Univ Syst, Texas A&M AgriLife Res, Overton, TX 75684 USA. EM r-randel@tamu.edu FU Texas A&M AgriLife Research; Mississippi Agricultural and Forestry Experiment Station FX Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This project was supported in part by Texas A&M AgriLife Research and the Mississippi Agricultural and Forestry Experiment Station. NR 47 TC 2 Z9 2 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 IS 2 BP 602 EP 609 DI 10.2527/jas2015-9635 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RA UT WOS:000374689900015 PM 27065130 ER PT J AU Villalba, JJ Spackman, C Goff, BM Klotz, JL Griggs, T MacAdam, JW AF Villalba, J. J. Spackman, C. Goff, B. M. Klotz, J. L. Griggs, T. MacAdam, J. W. TI Interaction between a tannin-containing legume and endophyte-infected tall fescue seed on lambs' feeding behavior and physiology SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE condensed tannins; ergot alkaloids; ingestive behavior; intake; prolactin ID SAINFOIN ONOBRYCHIS-VICIIFOLIA; CONDENSED TANNINS; BIRDSFOOT-TREFOIL; ERGOT ALKALOIDS; REED CANARYGRASS; FORAGE INTAKE; SHEEP; STEERS; PERFORMANCE; TOXICOSIS AB It was hypothesized that a tannin-rich legume such as sainfoin attenuates the negative postingestive effects of ergot alkaloids in tall fescue. Thirty-two 4-mo-old lambs were individually penned and randomly assigned to a 2 x 2 factorial arrangement with 2 legume species, sainfoin (SAN; 2.9% condensed tannins) or cicer milkvetch (CIC; without tannins) and a mixed ration containing tall fescue seed (50: 30: 20 seed: beet pulp: alfalfa) with 2 levels of endophyte infection (endophyte-infected tall fescue seed [E+; 3,150 ug/L ergovaline] or endophyte-free tall fescue seed [E-]). For a 10-d baseline period, half of the lambs were fed SAN and half were fed CIC and all lambs had ad libitum amounts of E-. In an ensuing 10-d experimental period, the protocol was the same except half of the lambs fed SAN or CIC received E+ instead of E-. Subsequently, all lambs could choose between their respective legume and seed-containing ration and between E+ and E-. Finally, an in vitro radial diffusion assay was conducted to determine whether tannins isolated from SAN would bind to alkaloids isolated from E+. All groups consumed similar amounts of E-during baseline period (P > 0.10), but lambs ate more E-than E+ during the experimental period (P < 0.05) and lambs offered SAN ate more E+ than lambs offered CIC (P < 0.05). Groups fed E-during the baseline and experimental periods had similar rectal temperatures (P > 0.10), but lambs fed E+ had lower rectal temperatures per gram of feed ingested when supplemented with SAN than with CIC (P < 0.05). Lambs fed E+ had greater concentrations of hemoglobin and more red blood cells than lambs fed E(P < 0.05), but plasmatic concentrations of cortisol and prolactin did not differ among treatments (P > 0.10). All lambs preferred their treatment ration over their treatment legume, but lambs in the SAN and E+ treatment ate more legume + ration than lambs in the CIC and E+ (CIC-E+; P < 0.05) treatment. All lambs preferred Eover E+, but lambs in the CIC-E+ treatment ate the least amount of E+ (P < 0.05). Binding of isolated SAN tannins to protein was reduced by the E+ isolate (P < 0.05), suggesting a tannin-alkaloid complexation but only from tannins extracted from SAN fed early in the experimental period. In summary, SAN supplementation increased intake of and preference for E+ and reduced rectal temperatures relative to CIC supplementation. Our results suggest that these effects were mediated by the condensed tannins in SAN through alkaloid inactivation, an interaction that declined with plant maturity. C1 [Villalba, J. J.; Spackman, C.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Goff, B. M.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Klotz, J. L.] ARS, USDA, Lexington, KY 40546 USA. [Griggs, T.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [MacAdam, J. W.] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. RP Villalba, JJ (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM juan.villalba@usu.edu RI Villalba, Juan/A-5836-2010 OI Villalba, Juan/0000-0001-8868-8468 FU Utah Agricultural Experiment Station; Utah Irrigated Pasture Grants Program FX This research was supported by grants from the Utah Agricultural Experiment Station, the Utah Irrigated Pasture Grants Program. This paper is published with the approval of the Director, Utah Agricultural Experiment Station, and Utah State University, as journal paper number 8844. NR 60 TC 0 Z9 0 U1 2 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 IS 2 BP 845 EP 857 DI 10.2527/jas2015-9790 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RA UT WOS:000374689900040 PM 27065155 ER PT J AU Vogeler, JC Cohen, WB AF Vogeler, J. C. Cohen, W. B. TI A review of the role of active remote sensing and data fusion for characterizing forest in wildlife habitat models SO REVISTA DE TELEDETECCION LA English DT Review DE wildlife habitat; forest; lidar; radar; predictive maps ID SYNTHETIC-APERTURE RADAR; AIRBORNE LIDAR; VEGETATION STRUCTURE; SPECIES-DIVERSITY; SQUIRREL HABITAT; NESTING HABITAT; SCALE; BIRD; BIOMASS; CONSERVATION AB Spatially explicit maps of wildlife habitat relationships have proven to be valuable tools for conservation and management applications including evaluating how and which species may be impacted by large scale climate change, ongoing fragmentation of habitat, and local land-use practices. Studies have turned to remote sensing datasets as a way to characterize vegetation for the examination of habitat selection and for mapping realized relationships across the landscape. Potentially one of the more difficult habitat types to try to characterize with remote sensing are the vertically and horizontally complex forest systems. Characterizing this complexity is needed to explore which aspects may represent driving and/or limiting factors for wildlife species. Active remote sensing data from lidar and radar sensors has thus caught the attention of the forest wildlife research and management community in its potential to represent three dimensional habitat features. The purpose of this review was to examine the applications of active remote sensing for characterizing forest in wildlife habitat studies through a keyword search within Web of Science. We present commonly used active remote sensing metrics and methods, discuss recent advances in characterizing aspects of forest habitat, and provide suggestions for future research in the area of new remote sensing data/techniques that could benefit forest wildlife studies that are currently not represented or may be underutilized within the wildlife literature. We also highlight the potential value in data fusion of active and passive sensor data for representing multiple dimensions and scales of forest habitat. While the use of remote sensing has increased in recent years within wildlife habitat studies, continued communication between the remote sensing, forest management, and wildlife communities is vital to ensure appropriate data sources and methods are understood and utilized, and so that creators of mapping products may better realize the needs of secondary users. C1 [Vogeler, J. C.] Oregon State Univ, Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Vogeler, J. C.] Univ Minnesota, Dept Forest Resources, 115 Green Hall, St Paul, MN 55108 USA. [Cohen, W. B.] US Forest Serv, USDA, Pacific NW Res Stn, 3200 NW Jefferson Way, Corvallis, OR 97331 USA. RP Vogeler, JC (reprint author), Oregon State Univ, Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA.; Vogeler, JC (reprint author), Univ Minnesota, Dept Forest Resources, 115 Green Hall, St Paul, MN 55108 USA. EM jvogeler@umn.edu NR 66 TC 0 Z9 0 U1 16 U2 23 PU ASOC ESPANOLA TELEDETECCION PI VALLADOLID PA C/O JOSE LUIS CASANOVA, EDITOR, PRADO DE LA MAGDALENA, S-N, VALLADOLID, 00000, SPAIN SN 1133-0953 EI 1988-8740 J9 REV TELEDETEC JI Rev. Teledetec. PD FEB PY 2016 IS 45 SI SI BP 1 EP 14 DI 10.4995/raet.2016.3981 PG 14 WC Remote Sensing SC Remote Sensing GA DK1KF UT WOS:000374670200001 ER PT J AU Hevia, A Alvarez-Gonzalez, JG Ruiz-Fernandez, E Prendes, C Ruiz-Gonzalez, AD Majada, J Gonzalez-Ferreiro, E AF Hevia, A. Alvarez-Gonzalez, J. G. Ruiz-Fernandez, E. Prendes, C. Ruiz-Gonzalez, A. D. Majada, J. Gonzalez-Ferreiro, E. TI Modelling canopy fuel and forest stand variables and characterizing the influence of thinning in the stand structure using airborne LiDAR SO REVISTA DE TELEDETECCION LA English DT Article DE Pinus pinaster; Airborne Laser Scanning (ALS); fuel management; canopy fuel load; canopy bulk density; canopy base height ID DISCRETE-RETURN LIDAR; PINUS-PINASTER AIT.; RADIATA D. DON; FIRE MANAGEMENT; STEM VOLUME; LOW-DENSITY; CROWN FIRE; NW SPAIN; PLANTATIONS; TREE AB Forest fires are a major threat in NW Spain. The importance and frequency of these events in the area suggests the need for fuel management programs to reduce the spread and severity of forest fires. Thinning treatments can contribute for fire risk reduction, because they cut off the horizontal continuity of forest fuels. Besides, it is necessary to conduct a fire risk management based on the knowledge of fuel allocation, since fire behaviour and fire spread study is dependent on the spatial factor. Therefore, mapping fuel for different silvicultural scenarios is essential. Modelling forest variables and forest structure parameters from LiDAR technology is the starting point for developing spatially-explicit maps. This is essential in the generation of fuel maps since field measurements of canopy fuel variables is not feasible. In the present study, we evaluated the potential of LiDAR technology to estimate canopy fuel variables and other stand variables, as well as to identify structural differences between silvicultural managed and unmanaged P. pinaster Ait. stands. Independent variables (LiDAR metrics) of greater explanatory significance were identified and regression analyses indicated strong relationships between those and field-derived variables (R-2 varied between 0.86 and 0.97). Significant differences were found in some LiDAR metrics when compared thinned and unthinned stands. Results showed that LiDAR technology allows to model canopy fuel and stand variables with high precision in this species, and provides useful information for identifying areas with and without silvicultural management. C1 [Hevia, A.; Prendes, C.; Majada, J.] Forest & Wood Technol Res Ctr CETEMAS, Finca Expt La Mata S-N, Grado 33820, Spain. [Alvarez-Gonzalez, J. G.; Ruiz-Fernandez, E.; Ruiz-Gonzalez, A. D.; Gonzalez-Ferreiro, E.] Univ Santiago de Compostela, Dept Agroforestry Engn, Sustainable Forest Management Unit UXFS, C Benigno Ledo S-N, Lugo 27002, Spain. [Gonzalez-Ferreiro, E.] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Gonzalez-Ferreiro, E.] US Forest Serv, LARSE, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Hevia, A (reprint author), Forest & Wood Technol Res Ctr CETEMAS, Finca Expt La Mata S-N, Grado 33820, Spain. EM ahevia@cetemas.es RI Gonzalez-Ferreiro, Eduardo/Q-9709-2016 OI Gonzalez-Ferreiro, Eduardo/0000-0002-4565-2155 NR 53 TC 0 Z9 0 U1 3 U2 3 PU ASOC ESPANOLA TELEDETECCION PI VALLADOLID PA C/O JOSE LUIS CASANOVA, EDITOR, PRADO DE LA MAGDALENA, S-N, VALLADOLID, 00000, SPAIN SN 1133-0953 EI 1988-8740 J9 REV TELEDETEC JI Rev. Teledetec. PD FEB PY 2016 IS 45 SI SI BP 41 EP 55 DI 10.4995/raet.2016.3979 PG 15 WC Remote Sensing SC Remote Sensing GA DK1KF UT WOS:000374670200004 ER PT J AU Hutto, RL Keane, RE Sherriff, RL Rota, CT Eby, LA Saab, VA AF Hutto, Richard L. Keane, Robert E. Sherriff, Rosemary L. Rota, Christopher T. Eby, Lisa A. Saab, Victoria A. TI Toward a more ecologically informed view of severe forest fires SO ECOSPHERE LA English DT Article DE early succession; ecological integrity; ecological system; fire management; fire regime; forest resilience; forest restoration; severe fire; wildfire ID CAVITY-NESTING BIRDS; WHITE-HEADED WOODPECKERS; WILDLAND-URBAN INTERFACE; ROCKY-MOUNTAIN FORESTS; WESTERN NORTH-AMERICA; GLACIER-NATIONAL-PARK; COLORADO-FRONT-RANGE; CONIFER FORESTS; BURN SEVERITY; HABITAT ASSOCIATIONS AB We use the historical presence of high-severity fire patches in mixed-conifer forests of the western United States to make several points that we hope will encourage development of a more ecologically informed view of severe wildland fire effects. First, many plant and animal species use, and have sometimes evolved to depend on, severely burned forest conditions for their persistence. Second, evidence from fire history studies also suggests that a complex mosaic of severely burned conifer patches was common historically in the West. Third, to maintain ecological integrity in forests born of mixed-severity fire, land managers will have to accept some severe fire and maintain the integrity of its aftermath. Lastly, public education messages surrounding fire could be modified so that people better understand and support management designed to maintain ecologically appropriate sizes and distributions of severe fire and the complex early-seral forest conditions it creates. C1 [Hutto, Richard L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Keane, Robert E.] US Forest Serv, USDA, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT USA. [Sherriff, Rosemary L.] Humboldt State Univ, Dept Geog, Arcata, CA 95521 USA. [Rota, Christopher T.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO USA. [Eby, Lisa A.] Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA. [Saab, Victoria A.] US Forest Serv, USDA, Rocky Mt Res Stn, Bozeman, MT USA. RP Hutto, RL (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. EM hutto@mso.umt.edu NR 102 TC 2 Z9 2 U1 20 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD FEB PY 2016 VL 7 IS 2 AR e01255 DI 10.1002/ecs2.1255 PG 13 WC Ecology SC Environmental Sciences & Ecology GA DK4NX UT WOS:000374896300008 ER PT J AU Chung, SY Reed, S AF Chung, Si-Yin Reed, Shawndrika TI Removing Peanut Allergen Ara h 1 from Peanut Extracts Using p-Aminobenzamidine SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY MAR 04-07, 2016 CL Los Angeles, CA SP Amer Acad Allergy, Asthma & Immunol C1 [Chung, Si-Yin; Reed, Shawndrika] USDA ARS, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 SU S MA 776 BP AB238 EP AB238 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DK6BI UT WOS:000375005403222 ER PT J AU Hurlburt, BK McBride, J Pote, S Chruszcz, M Maleki, SJ AF Hurlburt, Barry K. McBride, Jane Pote, Swanandi Chruszcz, Maksymilian Maleki, Soheila J. TI Ligand Binding Preferences of Pathogenesis-Related Class 10 (PR-10) Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY MAR 04-07, 2016 CL Los Angeles, CA SP Amer Acad Allergy, Asthma & Immunol C1 [Hurlburt, Barry K.; McBride, Jane; Maleki, Soheila J.] USDA ARS, SRRC, New Orleans, LA USA. [Pote, Swanandi; Chruszcz, Maksymilian] Univ S Carolina, Columbia, SC 29208 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 SU S MA 879 BP AB268 EP AB268 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DK6BI UT WOS:000375005404075 ER PT J AU Maleki, SJ Cheng, H Wolf, JC Teuber, SS Schein, C Grimm, CC Hurlburt, BK AF Maleki, Soheila J. Cheng, Hsiaopo Wolf, John C. Teuber, Suzanne S. Schein, Catherine Grimm, Casey C. Hurlburt, Barry K. TI Cross-Reactivity Among Peanut and Tree Nut Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY MAR 04-07, 2016 CL Los Angeles, CA SP Amer Acad Allergy, Asthma & Immunol C1 [Maleki, Soheila J.; Cheng, Hsiaopo; Wolf, John C.; Grimm, Casey C.; Hurlburt, Barry K.] USDA ARS SRRC, New Orleans, LA USA. [Teuber, Suzanne S.] UC Davis Sch Med, Davis, CA USA. [Schein, Catherine] Fdn Appl Mol Evolut, Gainesville, FL USA. [Schein, Catherine] UTMB, Galveston, TX USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 SU S MA 771 BP AB237 EP AB237 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DK6BI UT WOS:000375005403217 ER PT J AU Mattison, CP Khurana, T Tarver, M Florane, C Grimm, CC Pakala, S Cottone, C Riegel, C Slater, JE AF Mattison, Christopher P. Khurana, Taruna Tarver, Matthew Florane, Christopher Grimm, Casey C. Pakala, Suman Cottone, Carrie Riegel, Claudia Slater, Jay E. TI Termite Proteins Cross-React with Cockroach Allergens SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY MAR 04-07, 2016 CL Los Angeles, CA SP Amer Acad Allergy, Asthma & Immunol C1 [Mattison, Christopher P.; Florane, Christopher; Grimm, Casey C.] USDA ARS, SRRC, New Orleans, LA USA. [Khurana, Taruna; Slater, Jay E.] US FDA, CBER, OVRR, DBPAP, Silver Spring, MD USA. [Tarver, Matthew] Bayer CropSci, West Sacramento, CA USA. [Pakala, Suman] Univ Georgia, Athens, GA 30602 USA. [Cottone, Carrie; Riegel, Claudia] New Orleans Mosquito Termite & Rodent Control Boa, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 SU S MA 872 BP AB266 EP AB266 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DK6BI UT WOS:000375005404068 ER PT J AU Zhang, YZ Du, WX Fan, YT Nadeau, KC McHugh, TH AF Zhang, Yuzhu Du, Wen-Xian Fan, Yuting Nadeau, Kari C. McHugh, Tara H. TI Prediction and Identification of Korean Pine (Pinus koraiensis) Vicilin As a Food SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY MAR 04-07, 2016 CL Los Angeles, CA SP Amer Acad Allergy, Asthma & Immunol C1 [Zhang, Yuzhu; Du, Wen-Xian; Fan, Yuting; McHugh, Tara H.] USDA ARS, PWA, WRRC, Albany, CA USA. [Fan, Yuting] Jiangnan Univ, Wuxi, Peoples R China. [Nadeau, Kari C.] Stanford Univ, Sch Med, Pediat Allergy Immunol, Stanford, CA 94305 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2016 VL 137 IS 2 SU S MA 873 BP AB267 EP AB267 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA DK6BI UT WOS:000375005404069 ER PT J AU Weissmann, S Ma, FF Furuyama, K Gierse, J Berg, H Shao, Y Taniguchi, M Allen, DK Brutnell, TP AF Weissmann, Sarit Ma, Fangfang Furuyama, Koki Gierse, James Berg, Howard Shao, Ying Taniguchi, Mitsutaka Allen, Doug K. Brutnell, Thomas P. TI Interactions of C-4 Subtype Metabolic Activities and Transport in Maize Are Revealed through the Characterization of DCT2 Mutants SO PLANT CELL LA English DT Article ID BUNDLE-SHEATH CHLOROPLASTS; C4 MESOPHYLL CHLOROPLASTS; ZEA-MAYS-L; MALATE DECARBOXYLATION; PHOSPHOENOLPYRUVATE CARBOXYKINASE; GLUTAMATE-DEHYDROGENASE; DEVELOPMENTAL DYNAMICS; INORGANIC-PHOSPHATE; PANICUM-MILIACEUM; CO2 ASSIMILATION AB C-4 photosynthesis in grasses requires the coordinated movement of metabolites through two specialized leaf cell types, mesophyll (M) and bundle sheath (BS), to concentrate CO2 around Rubisco. Despite the importance of transporters in this process, few have been identified or rigorously characterized. In maize (Zea mays), DCT2 has been proposed to function as a plastid-localizedmalate transporter and is preferentially expressed in BS cells. Here, we characterized the role of DCT2 in maize leaves using Activator-tagged mutant alleles. Our results indicate that DCT2 enables the transport of malate into the BS chloroplast. Isotopic labeling experiments show that the loss of DCT2 results in markedly different metabolic network operation and dramatically reduced biomass production. In the absence of a functioning malate shuttle, dct2 lines survive through the enhanced use of the phosphoenolpyruvate carboxykinase carbon shuttle pathway that in wild-type maize accounts for; 25% of the photosynthetic activity. The results emphasize the importance of malate transport during C-4 photosynthesis, define the role of a primary malate transporter in BS cells, and support a model for carbon exchange between BS and M cells in maize. C1 [Weissmann, Sarit; Ma, Fangfang; Gierse, James; Berg, Howard; Shao, Ying; Allen, Doug K.; Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Furuyama, Koki; Taniguchi, Mitsutaka] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan. [Gierse, James; Allen, Doug K.] USDA ARS, St Louis, MO 63132 USA. RP Allen, DK; Brutnell, TP (reprint author), Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA.; Allen, DK (reprint author), USDA ARS, St Louis, MO 63132 USA. EM doug.allen@ars.usda.gov; tbrutnell@danforthcenter.org RI Allen, Doug/M-2836-2013; Berg, R. Howard/M-2839-2013; Brutnell, Thomas/M-2840-2013; OI Allen, Doug/0000-0001-8599-8946; Berg, R. Howard/0000-0001-5081-2769; Brutnell, Thomas/0000-0002-3581-8211; Weissmann, Sarit/0000-0001-9608-0866; Taniguchi, Mitsutaka/0000-0001-6485-2462 FU National Science Foundation [EF-1105249, IOS-092270, IOS-1127017, DBI-1427621, DBI-0521250]; Department of Energy [DE-AR0000202]; USDA-ARS FX We acknowledge the support of the National Science Foundation (EF-1105249; IOS-092270, IOS-1127017), the Department of Energy (DE-AR0000202), and the USDA-ARS. Mass spectrometry and microscopy were performed in the Proteomics and Mass Spectrometry Core and Integrated Microscopy Core Facilities at the Donald Danforth Plant Science Center using instrumentation in part supported by National Science Foundation grants (DBI-1427621 and DBI-0521250 for acquisition of QTRAP LC-MS/MS instruments). We thank Greg Ziegler for help with the R scripts, Kaitlin McNally for help with the screen, and Kevin Ahern for help with the field genetics. Any product or trademark mentioned here does not imply a warranty, guarantee, or endorsement by the authors or their affiliations over other suitable products. NR 69 TC 2 Z9 2 U1 5 U2 14 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD FEB PY 2016 VL 28 IS 2 BP 466 EP 484 DI 10.1105/tpc.15.00497 PG 19 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA DL1SU UT WOS:000375413300014 PM 26813621 ER PT J AU Li, CL Bai, GH Chao, SM Carver, B Wang, ZH AF Li, Chunlian Bai, Guihua Chao, Shiaoman Carver, Brett Wang, Zhonghua TI Single nucleotide polymorphisms linked to quantitative trait loci for grain quality traits in wheat SO CROP JOURNAL LA English DT Article DE Triticum aestivum; Epistasis; Grain quality traits; QTL x environments interaction; SNP ID TRITICUM-AESTIVUM L.; QTL ANALYSIS; BREAD WHEAT; KERNEL HARDNESS; WINTER-WHEAT; PROTEIN-CONTENT; AGRONOMIC TRAITS; DOUGH STRENGTH; COMMON WHEAT; DURUM-WHEAT AB Wheat (Triticum aestivum L.) grain quality traits that are controlled by quantitative traits loci (QTL) define suitable growing areas and potential end-use products of a wheat cultivar. To dissect QTL for these traits including protein content (GPC); test weight (TW); single kernel characterization system (SKCS)-estimated kernel weight (SKW); kernel diameter (KD); kernel hardness measured by near-infrared reflectance spectroscopy (NIRS) hardness index (NHI); and SKCS-hardness index (SHI), a high-density genetic map with single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) markers was developed using recombinant inbred lines (RILs) derived from Ning7840 x Clark. The RILs were evaluated for these quality traits in seven Oklahoma environments from 2001 to 2003. A total of 41 QTL with additive effects on different traits were mapped on most wheat chromosomes, excluding 1A, 2A, 3D, 4D, 6D, and 7B. Seven chromosome regions showed either tightly linked QTL or QTL with pleiotropic effects on two to four traits. Ten pairs of QTL showed additive x additive effects (AA), four QTL were involved in additive x environment (AE) effects, and one was involved in AAE effects. Two to eleven QTL for each of the six traits and 139 tightly linked markers to these QTL were identified. The findings shed light on the inheritance of wheat grain quality traits and provide DNA markers for manipulating these important traits to improve quality of new wheat cultivars. Production and hosting by Elsevier B.V. on behalf of Crop Science Society of China and Institute of Crop Science, CAAS. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Li, Chunlian; Wang, Zhonghua] Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China. [Li, Chunlian] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Li, Chunlian; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] ARS, Hard Winter Wheat Genet Res Unit, USDA, Manhattan, KS 66506 USA. [Chao, Shiaoman] ARS, Cereal Crops Res Unit, USDA, Fargo, ND USA. [Carver, Brett] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. RP Bai, GH (reprint author), ARS, Hard Winter Wheat Genet Res Unit, USDA, Manhattan, KS 66506 USA. EM gbai@ksu.edu NR 43 TC 0 Z9 0 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD FEB PY 2016 VL 4 IS 1 BP 1 EP 11 DI 10.1016/j.cj.2015.10.002 PG 11 WC Agronomy SC Agriculture GA DG3VE UT WOS:000371997800001 ER PT J AU Schaller, AM AF Schaller, A. M. TI Impacts of Western North Carolina Grazing Clinics on Producer Pasture Management. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Interagency Collaboration; Producer educational methods; Grazing plan adoption C1 [Schaller, A. M.] USDA, Nat Resources Conservat Serv, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 016 BP 8 EP 8 DI 10.2527/ssasas2015-016 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900017 ER PT J AU Altman, AW Vanzant, ES Carroll, JA Sanchez, NCB McLeod, KR AF Altman, A. W. Vanzant, E. S. Carroll, J. A. Sanchez, N. C. Burdick McLeod, K. R. TI Heat production, respiratory quotient, and methane loss subsequent to LPS challenge in beef heifers SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE lipopolysaccharide; calorimetry; heifers C1 [Altman, A. W.; Vanzant, E. S.; McLeod, K. R.] Univ Kentucky, Lexington, KY USA. [Carroll, J. A.; Sanchez, N. C. Burdick] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 040 BP 20 EP 20 DI 10.2527/ssasas2015-040 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900041 ER PT J AU Sanchez, NCB Broadway, PR Carroll, JA Gart, EV Bryan, LK Lawhon, SD AF Sanchez, N. C. Burdick Broadway, P. R. Carroll, J. A. Gart, E. V. Bryan, L. K. Lawhon, S. D. TI Sexually dimorphic innate immune responses but not tissue Salmonella translocation patterns in pigs exposed to an oral Salmonella challenge. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Innate immunity; Salmonella; sexual dimorphism C1 [Sanchez, N. C. Burdick; Broadway, P. R.; Carroll, J. A.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. [Gart, E. V.; Bryan, L. K.; Lawhon, S. D.] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA. RI Lawhon, Sara/G-5147-2011 OI Lawhon, Sara/0000-0001-9154-8909 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 061 BP 30 EP 30 DI 10.2527/ssasas2015-061 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900062 ER PT J AU Branco, RAD Neuendorff, DA Schmidt, SE Sanchez, NCB Carroll, JA Welsh, TH Randel, RD AF Branco, R. A. d'Orey Neuendorff, D. A. Schmidt, S. E. Sanchez, N. C. Burdick Carroll, J. A. Welsh, T. H., Jr. Randel, R. D. TI Influence of prenatal stress on insulin response to a glucose challenge in yearling Brahman bulls. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Prenatal Stress; Insulin; Cattle C1 [Branco, R. A. d'Orey; Schmidt, S. E.; Welsh, T. H., Jr.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Branco, R. A. d'Orey; Neuendorff, D. A.] Texas A&M AgriLife Res, Overton, TX USA. [Sanchez, N. C. Burdick; Carroll, J. A.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. [Randel, R. D.] Texas A&M AgriLife Res Overton, Overton, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 066 BP 33 EP 33 DI 10.2527/ssasas2015-066 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900067 ER PT J AU Broadway, PR Carroll, JA Sanchez, NCB Roberts, SL Sharon, KP Richeson, JT Corley, JR AF Broadway, P. R. Carroll, J. A. Sanchez, N. C. Burdick Roberts, S. L. Sharon, K. P. Richeson, J. T. Corley, J. R. TI Yeast probiotic supplementation mitigates some of the negative effects of heat stress in feedlot heifers. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE heat stress; yeast; beef C1 [Broadway, P. R.; Carroll, J. A.; Sanchez, N. C. Burdick] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. [Roberts, S. L.; Richeson, J. T.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX USA. [Sharon, K. P.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. [Corley, J. R.] Phileo Lesaffre Anim Care, Cedar Rapids, IA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 069 BP 34 EP 35 DI 10.2527/ssasas2015-069 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900070 ER PT J AU Broadway, PR Carroll, JA Sanchez, NCB Gart, EV Bryan, LK Lawhon, SD AF Broadway, P. R. Carroll, J. A. Sanchez, N. C. Burdick Gart, E. V. Bryan, L. K. Lawhon, S. D. TI A probiotic bolus is ineffective in reducing Salmonella shedding in orally-inoculated weaned pigs. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE pig; probiotic; Salmonella C1 [Broadway, P. R.; Carroll, J. A.; Sanchez, N. C. Burdick] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. [Gart, E. V.; Bryan, L. K.; Lawhon, S. D.] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA. RI Lawhon, Sara/G-5147-2011 OI Lawhon, Sara/0000-0001-9154-8909 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 072 BP 36 EP 36 DI 10.2527/ssasas2015-072 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900073 ER PT J AU Carroll, JA Sanchez, NCB Broadway, PR Bass, BE Frank, JW AF Carroll, J. A. Sanchez, N. C. Burdick Broadway, P. R. Bass, B. E. Frank, J. W. TI Modulation of the acute phase response following a lipopolysaccharide challenge in pigs supplemented with an all-natural Saccharamyces cerevisiae fermentation product. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE acute phase response; lipopolysaccharide; Saccharomyces cerevisiae fermentation product C1 [Carroll, J. A.; Sanchez, N. C. Burdick; Broadway, P. R.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. [Bass, B. E.; Frank, J. W.] Diamond V, Cedar Rapids, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 076 BP 38 EP 38 DI 10.2527/ssasas2015-076 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900077 ER PT J AU Acharya, M Burke, JM Smyth, E Davis, A Lester, TD Rorie, RW AF Acharya, M. Burke, J. M. Smyth, E. Davis, A. Lester, T. D. Rorie, R. W. TI Effect of semen extender and storage temperature on ram sperm motility over time SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE artificial insemination; semen extenders; sheep C1 [Acharya, M.; Lester, T. D.; Rorie, R. W.] Univ Arkansas, Div Agr, Dept Anim Sci, Fayetteville, AR 72701 USA. [Burke, J. M.; Smyth, E.] USDA ARS, Booneville, AR USA. [Davis, A.] Murray State Univ, Murray, KY 42071 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 107 BP 53 EP 53 DI 10.2527/ssasas2015-107 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900108 ER PT J AU Beck, P Gadberry, MS Gunter, SA Jennings, J AF Beck, P. Gadberry, M. S. Gunter, S. A. Jennings, J. TI Matching forage systems with cow size and environment for Sustainable Cow-Calf production in the southern region SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Forage; Cow-calf; southern region C1 [Beck, P.] Univ Arkansas, Hope, AR USA. [Gadberry, M. S.; Jennings, J.] Univ Arkansas, Dept Anim Sci, Little Rock, AR 72204 USA. [Gunter, S. A.] USDA ARS, Woodward, OK USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2016 VL 94 SU 1 MA 129 BP 63 EP 63 DI 10.2527/ssasas2015-129 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1SQ UT WOS:000374694900130 ER PT J AU Rahman, KM Camp, ME Prasad, N McNeel, AK Levy, SE Bartol, FF Bagnell, CA AF Rahman, Kathleen M. Camp, Meredith E. Prasad, Nripesh McNeel, Anthony K. Levy, Shawn E. Bartol, Frank F. Bagnell, Carol A. TI Age and Nursing Affect the Neonatal Porcine Uterine Transcriptome SO BIOLOGY OF REPRODUCTION LA English DT Article DE developmental biology; gene expression; genomics; lactocrine; neonatal; nursing; porcine/pig; transcriptome; uterus ID PROGESTERONE-RECEPTOR GENE; FEMALE REPRODUCTIVE-TRACT; ENDOMETRIAL DEVELOPMENT; ESTROGEN-RECEPTOR; MOUSE UTERUS; MATRIX-METALLOPROTEINASE; GLAND DEVELOPMENT; EARLY-PREGNANCY; CELL-MIGRATION; DNA-SYNTHESIS AB The lactocrine hypothesis for maternal programming of neonatal development was proposed to describe a mechanism through which milk-borne bioactive factors, delivered from mother to nursing offspring, could affect development of tissues, including the uterus. Porcine uterine development, initiated before birth, is completed postnatally. However, age-and lactocrine-sensitive elements of the neonatal porcine uterine developmental program are undefined. Here, effects of age and nursing on the uterine transcriptome for 48 h from birth (Postnatal Day [PND] = 0) were identified using RNA sequencing (RNAseq). Uterine tissues were obtained from neonatal gilts (n = 4 per group) within 1 h of birth and before feeding (PND 0), or 48 h after nursing ad libitum (PND 2N) or feeding a commercial milk replacer (PND 2R). RNAseq analysis revealed differentially expressed genes (DEGs) associated with both age (PND 2N vs. PND 0; 3283 DEGs) and nursing on PND 2 (PND 2N vs PND 2R; 896 DEGs). Expression of selected uterine genes was validated using quantitative real-time PCR. Bioinformatic analyses revealed multiple biological processes enriched in response to both age and nursing, including cell adhesion, morphogenesis, and cell-cell signaling. Age-sensitive pathways also included estrogen receptor-alpha and hedgehog signaling cascades. Lactocrine-sensitive processes in nursed gilts included those involved in response to wounding, the plasminogen activator network and coagulation. Overall, RNAseq analysis revealed comprehensive age- and nursing-related transcriptomic differences in the neonatal porcine uterus and identified novel pathways and biological processes regulating uterine development. C1 [Rahman, Kathleen M.; Camp, Meredith E.; Bagnell, Carol A.] Rutgers State Univ, Dept Anim Sci, Endocrinol & Anim Biosci Program, New Brunswick, NJ 08903 USA. [Prasad, Nripesh; Levy, Shawn E.] HudsonAlpha Inst Biotechnol, Genom Serv Lab, Huntsville, AL USA. [McNeel, Anthony K.] ARS, USDA, Clay Ctr, NE USA. [Bartol, Frank F.] Auburn Univ, Dept Anat Physiol & Pharmacol, Cellular & Mol Biosci Program, Auburn, AL 36849 USA. RP Bagnell, CA (reprint author), Rutgers State Univ, Dept Anim Sci, 84 Lipman Dr, New Brunswick, NJ 08901 USA. EM bagnell@aesop.rutgers.edu FU NSF [EPS-1158862]; Steinetz Charitable Lead Unitrust; [USDA-NRI-2007-35203-18098]; [2013-67016-20523] FX Supported by USDA-NRI-2007-35203-18098 and 2013-67016-20523 (to F.F.B. and C.A.B.), NSF EPS-1158862 (to F.F.B.), and the Steinetz Charitable Lead Unitrust (to C.A.B.). Sequencing data were deposited in the Gene Expression Omnibus (GEO) repository under series accession number GSE72388. NR 79 TC 1 Z9 1 U1 0 U2 2 PU SOC STUDY REPRODUCTION PI MADISON PA 1691 MONROE ST,SUITE # 3, MADISON, WI 53711-2021 USA SN 0006-3363 EI 1529-7268 J9 BIOL REPROD JI Biol. Reprod. PD FEB 1 PY 2016 VL 94 IS 2 AR 46 DI 10.1095/biolreprod.115.136150 PG 13 WC Reproductive Biology SC Reproductive Biology GA DJ5PL UT WOS:000374261800010 PM 26632611 ER PT J AU Lee, U Joo, S Klopfenstein, NB Kim, MS AF Lee, Uk Joo, Sukhyun Klopfenstein, Ned B. Kim, Mee-Sook TI Efficacy of washing treatments in the reduction of post-harvest decay of chestnuts (Castanea crenata 'Tsukuba') during storage SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Castanea; disease; microbial population; post-harvest decay ID SATIVA MILL.; IRRADIATION; FRUIT; GAMMA AB This research evaluated the influence of different washing treatments (i.e., tap water, ozone, microbubbles, and ozone combined with microbubbles) on post-harvest decay of chestnuts (Castanea crenata 'Tsukuba') during storage. Overall, treatments with ozone and microbubbles significantly reduced the decay frequency and the associated microbial populations (aerobic bacteria, mold/filamentous fungi, and yeasts) during post-harvest storage compared with the traditional practice (tap water washing). Enhancing the efficacy of chestnut washing treatments will contribute to the improved storage of high-quality chestnuts. C1 [Lee, Uk] Korea Forest Res Inst, Div Special Purpose Trees, Suwon 16631, South Korea. [Joo, Sukhyun; Kim, Mee-Sook] Kookmin Univ, Dept Forestry Environm & Syst, Seoul 02727, South Korea. [Klopfenstein, Ned B.] US Forest Serv, USDA, RMRS, Moscow, ID 83843 USA. RP Kim, MS (reprint author), Kookmin Univ, Dept Forestry Environm & Syst, Seoul 02727, South Korea. EM mkim@kookmin.ac.kr NR 15 TC 0 Z9 0 U1 4 U2 4 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD FEB PY 2016 VL 96 IS 1 BP 1 EP 5 DI 10.1139/cjps-2015-0089 PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DJ1CE UT WOS:000373940000001 ER PT J AU Andrade, LHM Lugarini, C Oliveira, RAS Silva, LTR Marvulo, MFV Garcia, JE Dubey, JP Silva, JCR AF Andrade, Leontina H. M. Lugarini, Camile Oliveira, Rhaysa A. S. Silva, Luana T. R. Marvulo, Maria Fernanda V. Garcia, Jose E. Dubey, Jitender P. Silva, Jean C. R. TI Occurrence of antibodies to Toxoplasma gondii in wild birds from three Federal Conservation Units of Paraiba and Bahia, Brazil SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Toxoplasma gondii; toxoplasmosis; wild birds; modified agglutination test ID SPARROWS PASSER-DOMESTICUS; INFECTION; STATE; PERNAMBUCO; PREVALENCE; NORTHEAST; ANIMALS AB Surveillance and monitoring of wildlife pathogens are essential in the environmental context and human public health, as these animals act as sentinels, reflecting environmental changes early on, whath gives more efficient environmental monitoring and allows quick access to information on the conditions of area. Birds are important in the epidemiology and life cycle of Toxoplasma gondii, because their tissues are important source of protein in the diet of felids and humans. The objective was to determine antibodies to Toxoplasma gondii in wild birds from three Federal Conservation Units of the states of Paraiba and Bahia by Modified Agglutination Test (MAT). From December 2011 to October 2013, 222 wild birds of 67 species from 27 families and 12 Orders were captured with mist nets. Blood samples were then collected and the serum was separated by centrifugation. The sera were tested (MAT >= 1: 25) using formalin-fixed whole tachyzoites and 2-mercaptoethanol. Antibodies to T. gondii were found in 3 of 222 (1.3%) birds: in 1 of 16 (6.2%) white-lined tanager (Tachyphonus rufus, titer 50), in 1 of 5 (20%) gray-fronted dove (Leptotilla rufaxila, titer 50), and in 1 of 1 (100%) ashy-throated casiornis (Casiornis fuscus, titer 25). This is the first report of occurrence of antibodies to T. gondii in these tree bird species from two Federal Conservation Units. C1 [Andrade, Leontina H. M.; Lugarini, Camile; Oliveira, Rhaysa A. S.; Silva, Luana T. R.; Silva, Jean C. R.] Univ Fed Rural Pernambuco UFRPE, Dept Med Vet, Rua Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil. [Lugarini, Camile] Ctr Nacl Pesquisa & Conservacao Aves Silvestres C, Inst Chico Mendes Conservacao Biodiversidade ICMB, BR-230 Km 10, BR-58108012 Cabedelo, PB, Brazil. [Lugarini, Camile; Marvulo, Maria Fernanda V.; Silva, Jean C. R.] Inst Brasileiro Med Conservacao Triade, Rua Silveira Lobo 32,Cx Postal 48, BR-52061030 Recife, PE, Brazil. [Marvulo, Maria Fernanda V.] Fac Max Planck, Rodovia Joao Ceccon 60, BR-13331400 Indaiatuba, SP, Brazil. [Marvulo, Maria Fernanda V.] Univ Paulista Unip, Ave Comendador Enzo Ferrari 280, BR-13043900 Campinas, SP, Brazil. [Garcia, Jose E.] Univ Fed Pernambuco UFPE, Unidade Acad Vitoria, Rua Alto Reservatorio S-N, BR-55608680 Vitoria De Santo Antao, PE, Brazil. [Dubey, Jitender P.] USDA, Anim Parasit Dis Lab, Anim & Nat Resources Inst, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Andrade, LHM (reprint author), Univ Fed Rural Pernambuco UFRPE, Dept Med Vet, Rua Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil. EM hellen_mac_vet@hotmail.com NR 27 TC 0 Z9 0 U1 1 U2 3 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X EI 1678-5150 J9 PESQUI VET BRASIL JI Pesqui. Vet. Bras. PD FEB PY 2016 VL 36 IS 2 BP 103 EP 107 DI 10.1590/S0100-736X2016000200007 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DJ1VN UT WOS:000373993200007 ER PT J AU Medina, GN Montiel, N Segundo, FDS Sturza, D Ramirez-Medina, E Grubman, MJ de los Santos, T AF Medina, Gisselle N. Montiel, Nestor Segundo, Fayna Diaz-San Sturza, Diego Ramirez-Medina, Elizabeth Grubman, Marvin J. de los Santos, Teresa TI Evaluation of a Fiber-Modified Adenovirus Vector Vaccine against Foot-and-Mouth Disease in Cattle SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID NONSTRUCTURAL PROTEIN 2B; COXSACKIE B VIRUSES; KIDNEY-CELL LINE; DENDRITIC CELLS; SUBUNIT VACCINE; GENE-TRANSFER; SEROTYPE 5; CAPSID PROTEINS; IN-VITRO; PROTECTION AB Novel vaccination approaches against foot-and-mouth disease (FMD) include the use of replication-defective human adenovirus type 5 (Ad5) vectors that contain the capsid-encoding regions of FMD virus (FMDV). Ad5 containing serotype A24 capsid sequences (Ad5.A24) has proved to be effective as a vaccine against FMD in livestock species. However, Ad5-vectored FMDV serotype O1 Campos vaccine (Ad5.O1C.2B) provides only partial protection of cattle against homologous challenge. It has been reported that a fiber-modified Ad5 vector expressing Arg-Gly-Asp (RGD) enhances transduction of antigen-presenting cells (APC) in mice. In the current study, we assessed the efficacy of a fiber-modified Ad5 (Adt.O1C.2B.RGD) in cattle. Expression of FMDV capsid proteins was superior in cultured cells infected with the RGD-modified vector. Furthermore, transgene expression of Adt.O1C.2B.RGD was enhanced in cell lines that constitutively express integrin alpha(v)beta 6, a known receptor for FMDV. In contrast, capsid expression in cattle-derived enriched APC populations was not enhanced by infection with this vector. Our data showed that vaccination with the two vectors yielded similar levels of protection against FMD in cattle. Although none of the vaccinated animals had detectable viremia, FMDV RNA was detected in serum samples from animals with clinical signs. Interestingly, CD4(+) and CD8(+) gamma interferon (IFN-gamma)(+) cell responses were detected at significantly higher levels in animals vaccinated with Adt. O1C.2B.RGD than in animals vaccinated with Ad5.O1C.2B. Our results suggest that inclusion of an RGD motif in the fiber of Ad5-vectored FMD vaccine improves transgene delivery and cell-mediated immunity but does not significantly enhance vaccine performance in cattle. C1 [Medina, Gisselle N.; Montiel, Nestor; Segundo, Fayna Diaz-San; Sturza, Diego; Ramirez-Medina, Elizabeth; Grubman, Marvin J.; de los Santos, Teresa] ARS, Plum Isl Anim Dis Ctr, USDA, Northeast Area, Greenport, NY USA. [Montiel, Nestor; Sturza, Diego; Ramirez-Medina, Elizabeth] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr, Res Participat Program, Oak Ridge, TN USA. RP de los Santos, T (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Northeast Area, Greenport, NY USA. EM teresa.delossantos@ars.usda.gov FU Departments of Homeland Security [HSHQPM-13-X-00113]; USDA [HSHQPM-13-X-00113]; CRIS project (USDA) [1940-32000-057-00D]; PI-ADC Research Participation Program FX This work was supported by reimbursable interagency agreement HSHQPM-13-X-00113 between the Departments of Homeland Security and USDA, by CRIS project 1940-32000-057-00D (USDA), and by PI-ADC Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy and USDA. NR 53 TC 2 Z9 2 U1 2 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 EI 1556-679X J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD FEB PY 2016 VL 23 IS 2 BP 125 EP 136 DI 10.1128/CVI.00426-15 PG 12 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DI4SI UT WOS:000373489200006 PM 26607309 ER PT J AU Waters, WR Maggioli, MF Palmer, MV Thacker, TC McGill, JL Vordermeier, HM Berney-Meyer, L Jacobs, WR Larsen, MH AF Waters, W. Ray Maggioli, Mayara F. Palmer, Mitchell V. Thacker, Tyler C. McGill, Jodi L. Vordermeier, H. Martin Berney-Meyer, Linda Jacobs, William R., Jr. Larsen, Michelle H. TI Interleukin-17A as a Biomarker for Bovine Tuberculosis SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID T-CELL RESPONSES; (IFN-GAMMA)-INDUCED PROTEIN 10; VIRULENT MYCOBACTERIUM-BOVIS; GAMMA-INTERFERON PRODUCTION; DOUBLE DELETION MUTANT; GENE-EXPRESSION DATA; INFECTED CATTLE; BCG VACCINATION; TH17 CELLS; M. BOVIS AB T helper 17 (Th17)-associated cytokines are integral to the immune responses to tuberculosis, initiating both protective and harmful inflammatory responses. The aim of the present study was to evaluate applied aspects of interleukin-17 (IL-17) biology in the context of Mycobacterium bovis infection of cattle. Using transcriptome sequencing (RNA-Seq), numerous Th17-associated cytokine genes (including IL-17A, IL-17F, IL-22, IL-19, and IL-27) were upregulated >9-fold in response to purified protein derivative stimulation of peripheral blood mononuclear cells from experimentally M. bovis-infected cattle. Protective vaccines elicited IL-17A, IL-17F, IL-22, and IL-27 responses. Reduced IL-17A responses by vaccine recipients, compared to nonvaccinated animals, at 2.5 weeks after M. bovis challenge correlated with reduced disease burdens. Additionally, IL-17A and interferon gamma (IFN-gamma) responses were highly correlated and exhibited similar diagnostic capacities. The present findings support the use of Th17-associated cytokines as biomarkers of infection and protection in the immune responses to bovine tuberculosis. C1 [Waters, W. Ray; Maggioli, Mayara F.; Palmer, Mitchell V.; Thacker, Tyler C.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Maggioli, Mayara F.] Iowa State Univ, Coll Vet Med, Ames, IA USA. [McGill, Jodi L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Vordermeier, H. Martin] Anim & Plant Hlth Agcy, TB Res Grp, Addlestone, Surrey, England. [Berney-Meyer, Linda; Jacobs, William R., Jr.; Larsen, Michelle H.] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA. RP Waters, WR (reprint author), ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM ray.waters@ars.usda.gov RI APHA, Staff publications/E-6082-2010; Vordermeier, H Martin/C-6936-2011 FU Agriculture and Food Research Initiative [2011-67015-30736]; Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011-67015-30736] FX Agriculture and Food Research Initiative provided funding to W. Ray Waters and Mitchell V. Palmer under grant number 2011-67015-30736.; This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-30736 from the USDA National Institute of Food and Agriculture to W. Ray Waters and Mitchell V. Palmer. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. NR 58 TC 3 Z9 3 U1 5 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 EI 1556-679X J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD FEB PY 2016 VL 23 IS 2 BP 168 EP 180 DI 10.1128/CVI.00637-15 PG 13 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DI4SI UT WOS:000373489200011 PM 26677202 ER PT J AU Munoz-Erickson, TA Cutts, BB AF Munoz-Erickson, Tischa A. Cutts, Bethany B. TI Structural dimensions of knowledge-action networks for sustainability SO CURRENT OPINION IN ENVIRONMENTAL SUSTAINABILITY LA English DT Article ID NATURAL-RESOURCE MANAGEMENT; POLICY NETWORKS; CLIMATE-CHANGE; STAKEHOLDER ANALYSIS; SOCIAL NETWORKS; COLLABORATION; GOVERNANCE; ADAPTATION; INFORMATION; COOPERATION AB Research on the influence of social network structure over flows of knowledge in support of sustainability governance and action has recently flourished. These studies highlight three challenges to evaluating knowledge-action networks: first, defining boundaries; second, characterizing power distributions; and third, identifying obstacles to knowledge sharing and connectivity. We present concepts from social network analysis (SNA) commonly found to influence knowledge flows. We examine applications of SNA from across the social sciences and use the case of land governance in San Juan, Puerto Rico, as an illustration of how all three challenges affect knowledge action networks. SNA is a useful way to understand and overcome many challenges to knowledge flow and thus help improve informational governance strategies for sustainability. C1 [Munoz-Erickson, Tischa A.] US Forest Serv, USDA, Int Inst Trop Forestry, Rio Piedras, PR 00926 USA. [Cutts, Bethany B.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. RP Munoz-Erickson, TA (reprint author), US Forest Serv, USDA, Int Inst Trop Forestry, 1201 Jardin Bot Sur, Rio Piedras, PR 00926 USA. EM tamunozerickson@fs.fed.us FU USDA Forest Service, International Institute of Tropical Forestry; National Science Foundation [0948507]; USDA [ILLU-875-919] FX We are grateful to Katrine Soma and Paul Opdam for their useful comments on earlier versions of this manuscript. Support for this work was provided by the USDA Forest Service, International Institute of Tropical Forestry, the National Science Foundation under Grant number 0948507 (Urban Long-Term Research Area Exploratory) and the USDA under Grant number ILLU-875-919. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the view of the National Science Foundation (NSF) and the United States Department of Agriculture (USDA). NR 51 TC 1 Z9 1 U1 6 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1877-3435 EI 1877-3443 J9 CURR OPIN ENV SUST JI Curr. Opin. Environ. Sustain. PD FEB PY 2016 VL 18 BP 56 EP 64 DI 10.1016/j.cosust.2015.08.013 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DI5LP UT WOS:000373540900009 ER PT J AU Lin, BR Zhuo, K Chen, SY Hu, LL Sun, LH Wang, XH Zhang, LH Liao, JL AF Lin, Borong Zhuo, Kan Chen, Shiyan Hu, Lili Sun, Longhua Wang, Xiaohong Zhang, Lian-Hui Liao, Jinling TI A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system SO NEW PHYTOLOGIST LA English DT Article DE Arabidopsis thaliana; ferredoxin : thioredoxin reductase catalytic subunit; Meloidogyne javanica; pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI); reactive oxygen species (ROS); transthyretin-like protein (TTL) ID EXPRESSED SEQUENCE TAGS; THIOREDOXIN REDUCTASE; GENE-EXPRESSION; CYST-NEMATODE; PARASITIC NEMATODES; DISEASE RESISTANCE; MELOIDOGYNE-INCOGNITA; HETERODERA-SCHACHTII; HAEMONCHUS-CONTORTUS; ARABIDOPSIS-THALIANA AB Evidence is emerging that plant-parasitic nematodes can secrete effectors to interfere with the host immune response, but it remains unknown how these effectors can conquer host immune responses. Here, we depict a novel effector, MjTTL5, that could suppress plant immune response. Immunolocalization and transcriptional analyses showed that MjTTL5 is expressed specifically within the subventral gland of Meloidogyne javanica and up-regulated in the early parasitic stage of the nematode. Transgenic Arabidopsis lines expressing MjTTL5 were significantly more susceptible to M. javanica infection than wild-type plants, and vice versa, in planta silencing of MjTTL5 substantially increased plant resistance to M. javanica. Yeast two-hybrid, coimmunoprecipitation and bimolecular fluorescent complementation assays showed that MjTTL5 interacts specifically with Arabidopsis ferredoxin : thioredoxin reductase catalytic subunit (AtFTRc), a key component of host antioxidant system. The expression of AtFTRc is induced by the infection of M. javanica. Interaction between AtFTRc and MjTTL could drastically increase host reactive oxygen species-scavenging activity, and result in suppression of plant basal defenses and attenuation of host resistance to the nematode infection. Our results demonstrate that the host ferredoxin : thioredoxin system can be exploited cunningly by M. javanica, revealing a novel mechanism utilized by plant-parasitic nematodes to subjugate plant innate immunity and thereby promoting parasitism. C1 [Lin, Borong; Zhuo, Kan; Hu, Lili; Sun, Longhua; Liao, Jinling] South China Agr Univ, Lab Plant Nematol, Guangzhou 510642, Guangdong, Peoples R China. [Lin, Borong; Zhuo, Kan; Hu, Lili; Sun, Longhua; Zhang, Lian-Hui; Liao, Jinling] South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou 510642, Guangdong, Peoples R China. [Chen, Shiyan; Wang, Xiaohong] Cornell Univ, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Wang, Xiaohong] ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. [Zhang, Lian-Hui] Inst Mol & Cell Biol, 61 Biopolis Dr, Singapore 138673, Singapore. [Liao, Jinling] Guangdong Vocat Coll Ecol Engn, Guangzhou 510520, Guangdong, Peoples R China. RP Liao, JL (reprint author), South China Agr Univ, Lab Plant Nematol, Guangzhou 510642, Guangdong, Peoples R China.; Liao, JL (reprint author), South China Agr Univ, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou 510642, Guangdong, Peoples R China.; Zhang, LH (reprint author), Inst Mol & Cell Biol, 61 Biopolis Dr, Singapore 138673, Singapore.; Liao, JL (reprint author), Guangdong Vocat Coll Ecol Engn, Guangzhou 510520, Guangdong, Peoples R China. EM lianhui@imcb.a-star.edu.sg; jlliao@scau.edu.cn FU National Key Basic Research Program of China (973 Program) [2013CB127501]; National Nature Science Foundation of China [31171824]; Special Fund For Agro-Scientific Research In The Public Interest of China [201103018]; Pearl River Nova Program of Guangzhou [2014J2200069] FX We thank Professor Hong-bin Wang (Sun Yatsen University, China) for providing the anti-AtFTRc-antibody. This work was supported by grants from National Key Basic Research Program of China (973 Program, no. 2013CB127501), National Nature Science Foundation of China (no. 31171824), the Special Fund For Agro-Scientific Research In The Public Interest of China (no. 201103018) and the Pearl River Nova Program of Guangzhou (no. 2014J2200069). NR 73 TC 5 Z9 5 U1 16 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD FEB PY 2016 VL 209 IS 3 BP 1159 EP 1173 DI 10.1111/nph.13701 PG 15 WC Plant Sciences SC Plant Sciences GA DI3DI UT WOS:000373378000028 PM 26484653 ER PT J AU Fontana, L Villareal, DT Das, SK Smith, SR Meydani, SN Pittas, AG Klein, S Bhapkar, M Rochon, J Ravussin, E Holloszy, JO AF Fontana, Luigi Villareal, Dennis T. Das, Sai K. Smith, Steven R. Meydani, Simin N. Pittas, Anastassios G. Klein, Samuel Bhapkar, Manjushri Rochon, James Ravussin, Eric Holloszy, John O. CA CALERIE Study Grp TI Effects of 2-year calorie restriction on circulating levels of IGF-1, IGF-binding proteins and cortisol in nonobese men and women: a randomized clinical trial SO AGING CELL LA English DT Article DE calorie restriction; cancer; cortisol; IGF-1; IGFBP-1; weight loss ID SKIN TUMOR PROMOTION; GROWTH-FACTOR; FOOD RESTRICTION; DIETARY RESTRICTION; RHESUS-MONKEYS; ENERGY-INTAKE; HUMANS; MICE; PLASMA; CANCER AB Young-onset calorie restriction (CR) in rodents decreases serum IGF-1 concentration and increases serum corticosterone levels, which have been hypothesized to play major roles in mediating its anticancer and anti-aging effects. However, little is known on the effects of CR on the IGF-1 system and cortisol in humans. To test the sustained effects of CR on these key hormonal adaptations, we performed a multicenter randomized trial of a 2-year 25% CR intervention in 218 nonobese (body mass index between 22 and 27.8 kg m(-2)) young and middle-aged (20-50 years age range) men and women. Average CR during the first 6 months was 19.5 +/- 0.8% and 9.1 +/- 0.7% over the next 18 months of the study. Weight loss averaged 7.6 +/- 0.3 kg over the 2-years period of which 71% was fat mass loss (P < 0.0001). Average CR during the CR caused a significant 21% increase in serum IGFBP-1 and a 42% reduction in IGF-1: IGFBP-1 ratio at 2 years (P < 0.008), but did not change IGF-1 and IGF-1: IGFBP-3 ratio levels. Serum cortisol concentrations were slightly but significantly increased by CR at 1 year only (P = 0.003). Calorie restriction had no effect on serum concentrations of PDGF-AB and TGF beta-1. We conclude, on the basis of the present and previous findings, that, in contrast to rodents, humans do not respond to CR with a decrease in serum IGF-1 concentration or with a sustained and biological relevant increase in serum cortisol. However, long-term CR in humans significantly and persistently increases serum IGFBP-1 concentration. C1 [Fontana, Luigi; Villareal, Dennis T.; Klein, Samuel; Holloszy, John O.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA. [Fontana, Luigi] Univ Brescia, Sch Med, Dept Clin & Expt Sci, Brescia, Italy. [Fontana, Luigi] CEINGE Biotecnol Avanzate, Naples, Italy. [Villareal, Dennis T.] Michael E DeBakey VA Med Ctr, Baylor Coll Med, CTRID, Houston, TX USA. [Das, Sai K.; Meydani, Simin N.; Pittas, Anastassios G.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Boston, MA 02111 USA. [Smith, Steven R.; Ravussin, Eric] Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Smith, Steven R.] Florida Hosp, Sanford Burnham Med Res Inst, Translat Res Inst Metab & Diabet, Orlando, FL USA. [Bhapkar, Manjushri; Rochon, James] Duke Clin Res Inst, Durham, NC USA. [Rochon, James] Rho Fed Syst, Chapel Hill, NC USA. RP Fontana, L (reprint author), Washington Univ, Sch Med, 4566 Scott Ave,Campus Box 8113, St Louis, MO 63110 USA. EM lfontana@dom.wustl.edu FU National Institute on Aging [U01-AG-020487, U01-AG-020478, U01-AG-020480, U01-AG-022132]; National Institutes of Health [MO1-RR00036, P30-DK-056341, UL1RR024992] FX This study was supported by National Institute on Aging Cooperative Agreements U01-AG-020487, U01-AG-020478, U01-AG-020480, and U01-AG-022132 and National Institutes of Health Grants MO1-RR00036, P30-DK-056341 and UL1RR024992. NR 44 TC 11 Z9 11 U1 5 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1474-9718 EI 1474-9726 J9 AGING CELL JI Aging Cell PD FEB PY 2016 VL 15 IS 1 BP 22 EP 27 DI 10.1111/acel.12400 PG 6 WC Cell Biology; Geriatrics & Gerontology SC Cell Biology; Geriatrics & Gerontology GA DH6CQ UT WOS:000372879000003 PM 26443692 ER PT J AU Siderhurst, MS Park, SJ Buller, CN Jamie, IM Manoukis, NC Jang, EB Taylor, PW AF Siderhurst, Matthew S. Park, Soo J. Buller, Caitlyn N. Jamie, Ian M. Manoukis, Nicholas C. Jang, Eric B. Taylor, Phillip W. TI Raspberry Ketone Trifluoroacetate, a New Attractant for the Queensland Fruit Fly, Bactrocera Tryoni (Froggatt) SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Lures; Raspberry ketone trifluoroacetate; Raspberry ketone; Cuelure; Melolure; Bactrocera tryoni; Tephritidae; Bioassay; Horticultural pest; Tephritid; Fruit fly; Invasive ID MALE MELON FLY; NEW-SOUTH-WALES; DIPTERA TEPHRITIDAE; FLIES DIPTERA; DACUS-CUCURBITAE; CUE-LURE; AUSTRALIA; FORMATE; DORSALIS; DACINAE AB Queensland fruit fly, Bactrocera tryoni (Q-fly), is a major pest of horticultural crops in eastern Australia. Lures that attract male Q-fly are important for detection of incursions and outbreaks, monitoring of populations, and control by mass trapping and male annihilation. Cuelure, an analog of naturally occurring raspberry ketone, is the standard Q-fly lure, but it has limited efficacy compared with lures that are available for some other fruit flies such as methyl eugenol for B. dorsalis. Melolure is a more recently developed raspberry ketone analog that has shown better attraction than cuelure in some field studies but not in others. A novel fluorinated analog of raspberry ketone, raspberry ketone trifluoroacetate (RKTA), has been developed as a potential improvement on cuelure and melolure. RKTA placed on laboratory cages containing 2-week-old Q-flies elicited strong behavioral responses from males. Quantification of Q-fly responses in these cages, using digital images to estimate numbers of flies aggregated near different lures, showed RKTA attracted and arrested significantly more flies than did cuelure or melolure. RKTA shows good potential as a new lure for improved surveillance and control of Q-fly. C1 [Siderhurst, Matthew S.; Buller, Caitlyn N.] Eastern Mennonite Univ, Dept Chem, 1200 Pk Rd, Harrisonburg, VA 22802 USA. [Park, Soo J.; Jamie, Ian M.] Macquarie Univ, Dept Chem & Biomol Sci, N Ryde, NSW 2109, Australia. [Manoukis, Nicholas C.; Jang, Eric B.] USDA, ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Taylor, Phillip W.] Macquarie Univ, Dept Biol Sci, N Ryde, NSW 2109, Australia. RP Siderhurst, MS (reprint author), Eastern Mennonite Univ, Dept Chem, 1200 Pk Rd, Harrisonburg, VA 22802 USA. EM matthew.siderhurst@emu.edu RI Taylor, Phillip/E-4620-2011; Jamie, Ian/D-6992-2017 OI Taylor, Phillip/0000-0002-7574-7737; Jamie, Ian/0000-0003-0326-5074 FU Horticulture Innovation Australia Limited; Australian Government FX The authors thank Andrew Jessup, Lori Carvalho, Janice Nagata, Anca Chirvasuta, Erika Babikow, and Ian Koons for assistance with bioassays and chemical synthesis. This project was funded by Horticulture Innovation Australia Limited using the Summerfruit, Citrus, Vegetables, and Rubus levy and funds from the Australian Government. NR 39 TC 2 Z9 2 U1 8 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2016 VL 42 IS 2 BP 156 EP 162 DI 10.1007/s10886-016-0673-3 PG 7 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DH8AP UT WOS:000373015800008 PM 26922349 ER PT J AU Kandel, R Singh, HP Singh, BP Harris-Shultz, KR Anderson, WF AF Kandel, Raju Singh, Hari P. Singh, Bharat P. Harris-Shultz, Karen R. Anderson, William F. TI Assessment of Genetic Diversity in Napier Grass (Pennisetum purpureum Schum.) using Microsatellite, Single-Nucleotide Polymorphism and Insertion-Deletion Markers from Pearl Millet (Pennisetum glaucum [L.] R. Br.) SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Pennisetum purpureum; SSR marker; Polymorphism; Transferability; Genetic variability; UPGMA ID CROSS-SPECIES AMPLIFICATION; SEQUENCE REPEAT MARKERS; X-P-GLAUCUM; ASSISTED SELECTION; SSR-MARKERS; EST-SSRS; TRANSFERABILITY; ACCESSIONS; GERMPLASM; RAPD AB Napier grass (Pennisetum purpureum Schum.) is a well-established perennial fodder crop of African origin which recently has also drawn attention for its potential as biofuel feedstock. The absence of genome information in Napier grass limits the development of sequence-specific markers which often involves a high developmental cost. This study aimed to determine cross-species transferability of microsatellite markers between pearl millet (Pennisetum glaucum L.) and Napier grass and to assess the genetic diversity of Napier grass accessions. A total of 107 pearl millet microsatellite markers were tested of which 71 markers (66 %) showed successful cross-amplification. Only 29 markers were selected to study the genetic diversity of Napier grass accessions maintained at the US Department of Agriculture-Agriculture Research Service (USDA-ARS) Tifton, GA. A total of 108 alleles were identified among 99 accessions, a pearl millet line, and a sugarcane hybrid. The average polymorphic information content (PIC) value was 0.212 per marker, while Dice coefficient of similarity ranged from 0.50 to 1.0, indicating high genetic variability among accessions. The accessions with the lowest Dice coefficient of similarity values could be useful in breeding programs. The similarity coefficient of accessions equal to 1.00 is likely an indication of a single genotype being represented by two accessions with different names. This study provides an expanded set of microsatellite markers transferable from pearl millet to Napier grass that can be used to evaluate genetic diversity in Napier grass accessions. C1 [Kandel, Raju] Univ Wyoming, Sheridan Res & Extens Ctr, Sheridan, WY 82801 USA. [Singh, Hari P.; Singh, Bharat P.] Ft Valley State Univ, Agr Res Stn, Ft Valley, GA 31030 USA. [Harris-Shultz, Karen R.; Anderson, William F.] USDA ARS, Crop Genet & Breeding Res Unit, 115 Coastal Ways, Tifton, GA 31793 USA. RP Kandel, R (reprint author), Univ Wyoming, Sheridan Res & Extens Ctr, Sheridan, WY 82801 USA. EM rkandel@uwyo.edu FU USDA-NIFA FX This study was funded by USDA-NIFA grant awarded to Fort Valley State University NR 54 TC 0 Z9 0 U1 2 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD FEB PY 2016 VL 34 IS 1 BP 265 EP 272 DI 10.1007/s11105-015-0918-2 PG 8 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DH6TI UT WOS:000372924200023 ER PT J AU Liu, XL Huang, M Fan, B Buckler, ES Zhang, ZW AF Liu, Xiaolei Huang, Meng Fan, Bin Buckler, Edward S. Zhang, Zhiwu TI Iterative Usage of Fixed and Random Effect Models for Powerful and Efficient Genome-Wide Association Studies SO PLOS GENETICS LA English DT Article ID LINEAR MIXED MODELS; COMMON SNPS EXPLAIN; POPULATION-STRUCTURE; LARGE PROPORTION; HUMAN HEIGHT; STRATIFICATION; HERITABILITY; TOOL AB False positives in a Genome-Wide Association Study (GWAS) can be effectively controlled by a fixed effect and random effect Mixed Linear Model (MLM) that incorporates population structure and kinship among individuals to adjust association tests on markers; however, the adjustment also compromises true positives. The modified MLM method, Multiple Loci Linear Mixed Model (MLMM), incorporates multiple markers simultaneously as covariates in a stepwise MLM to partially remove the confounding between testing markers and kinship. To completely eliminate the confounding, we divided MLMM into two parts: Fixed Effect Model (FEM) and a Random Effect Model (REM) and use them iteratively. FEM contains testing markers, one at a time, and multiple associated markers as covariates to control false positives. To avoid model over-fitting problem in FEM, the associated markers are estimated in REM by using them to define kinship. The P values of testing markers and the associated markers are unified at each iteration. We named the new method as Fixed and random model Circulating Probability Unification (FarmCPU). Both real and simulated data analyses demonstrated that FarmCPU improves statistical power compared to current methods. Additional benefits include an efficient computing time that is linear to both number of individuals and number of markers. Now, a dataset with half million individuals and half million markers can be analyzed within three days. C1 [Liu, Xiaolei; Fan, Bin] Huazhong Agr Univ, Minist Educ, Coll Anim Sci & Technol, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan, Hubei, Peoples R China. [Liu, Xiaolei; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY USA. [Huang, Meng; Zhang, Zhiwu] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Buckler, Edward S.] ARS, USDA, Ithaca, NY USA. [Zhang, Zhiwu] Northeast Agr Univ, Dept Anim Sci, Harbin, Heilongjiang, Peoples R China. RP Zhang, ZW (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA.; Zhang, ZW (reprint author), Northeast Agr Univ, Dept Anim Sci, Harbin, Heilongjiang, Peoples R China. EM Zhiwu.Zhang@WSU.edu OI Buckler, Edward/0000-0002-3100-371X; Zhang, Zhiwu/0000-0002-5784-9684 FU China Scholarship Council; NSF-Plant Genome Program [DBI-0820619, IOS-1238014]; National Science Foundation of China [31072009, 31172192]; New Century Excellent Talents [NCET-11-0646] FX XL was supported by the China Scholarship Council (URL: http://en.csc.edu.cn) ESB was supported by the NSF-Plant Genome Program (DBI-0820619 and IOS-1238014) (URL: http://www.nsf.gov/pubs/2015/nsf15548/nsf15548.htm) BF was supported by the National Science Foundation of China (31072009, 31172192) (URL: http://www.nsfc.gov.cn/publish/portal1/), New Century Excellent Talents (NCET-11-0646). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 4 Z9 5 U1 9 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD FEB PY 2016 VL 12 IS 2 AR e1005767 DI 10.1371/journal.pgen.1005767 PG 24 WC Genetics & Heredity SC Genetics & Heredity GA DH1OI UT WOS:000372554100006 PM 26828793 ER PT J AU Zhou, Q Zhao, L Guan, YT AF Zhou, Quan Zhao, Liang Guan, Yongtao TI Strong Selection at MHC in Mexicans since Admixture SO PLOS GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; ADMIXED POPULATIONS; LOCAL-ANCESTRY; LATINO POPULATIONS; GENETIC-VARIATION; INDIVIDUALS; INFERENCE AB Mexicans are a recent admixture of Amerindians, Europeans, and Africans. We performed local ancestry analysis of Mexican samples from two genome-wide association studies obtained from dbGaP, and discovered that at the MHC region Mexicans have excessive African ancestral alleles compared to the rest of the genome, which is the hallmark of recent selection for admixed samples. The estimated selection coefficients are 0.05 and 0.07 for two datasets, which put our finding among the strongest known selections observed in humans, namely, lactase selection in northern Europeans and sickle-cell trait in Africans. Using inaccurate Amerindian training samples was a major concern for the credibility of previously reported selection signals in Latinos. Taking advantage of the flexibility of our statistical model, we devised a model fitting technique that can learn Amerindian ancestral haplotype from the admixed samples, which allows us to infer local ancestries for Mexicans using only European and African training samples. The strong selection signal at the MHC remains without Amerindian training samples. Finally, we note that medical history studies suggest such a strong selection at MHC is plausible in Mexicans. C1 [Zhou, Quan; Zhao, Liang; Guan, Yongtao] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Zhou, Quan; Zhao, Liang; Guan, Yongtao] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Zhou, Quan; Guan, Yongtao] Baylor Coll Med, Program Struct & Computat Biol & Mol Biophys, Houston, TX 77030 USA. [Guan, Yongtao] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. RP Guan, YT (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA.; Guan, YT (reprint author), Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA.; Guan, YT (reprint author), Baylor Coll Med, Program Struct & Computat Biol & Mol Biophys, Houston, TX 77030 USA.; Guan, YT (reprint author), Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. EM yongtaog@bcm.edu FU United States Department of Agriculture/Agricultural Research Service USDA/ARS [6250-51000-057]; National Institutes of Health [R01HG008157] FX This project is funded by United States Department of Agriculture/Agricultural Research Service USDA/ARS 6250-51000-057 and National Institutes of Health R01HG008157. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 4 Z9 4 U1 2 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD FEB PY 2016 VL 12 IS 2 AR e1005847 DI 10.1371/journal.pgen.1005847 PG 17 WC Genetics & Heredity SC Genetics & Heredity GA DH1OI UT WOS:000372554100035 PM 26863142 ER PT J AU Donaldson, TG de Leon, AAP Li, AI Castro-Arellano, I Wozniak, E Boyle, WK Hargrove, R Wilder, HK Kim, HJ Teel, PD Lopez, JE AF Donaldson, Taylor G. de Leon, Adalberto A. Perez Li, Andrew I. Castro-Arellano, Ivan Wozniak, Edward Boyle, William K. Hargrove, Reid Wilder, Hannah K. Kim, Hee J. Teel, Pete D. Lopez, Job E. TI Assessment of the Geographic Distribution of Ornithodoros turicata (Argasidae): Climate Variation and Host Diversity SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Article ID BORNE RELAPSING FEVER; AFRICAN-SWINE-FEVER; BORRELIA-TURICATAE; UNITED-STATES; NORTH-AMERICA; SOFT TICK; ACARI; TRANSMISSION; ANTIBODY; VECTORS AB Background Ornithodoros turicata is a veterinary and medically important argasid tick that is recognized as a vector of the relapsing fever spirochete Borrelia turicatae and African swine fever virus. Historic collections of O. turicata have been recorded from Latin America to the southern United States. However, the geographic distribution of this vector is poorly understood in relation to environmental variables, their hosts, and consequently the pathogens they transmit. Methodology Localities of O. turicata were generated by performing literature searches, evaluating records from the United States National Tick Collection and the Symbiota Collections of Arthropods Network, and by conducting field studies. Maximum entropy species distribution modeling (Maxent) was used to predict the current distribution of O. turicata. Vertebrate host diversity and GIS analyses of their distributions were used to ascertain the area of shared occupancy of both the hosts and vector. Conclusions and Significance Our results predicted previously unrecognized regions of the United States with habitat that may maintain O. turicata and could guide future surveillance efforts for a tick capable of transmitting high-consequence pathogens to human and animal populations. C1 [Donaldson, Taylor G.; Kim, Hee J.; Teel, Pete D.] Texas A&M AgriLife Res, Dept Entomol, College Stn, TX USA. [de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX USA. [de Leon, Adalberto A. Perez] Vet Pest Genom Ctr, Kerrville, TX USA. [Li, Andrew I.] USDA ARS, Invas Insects Biocontrol & Behav Lab, Beltsville, MD USA. [Castro-Arellano, Ivan] Texas State Univ, Dept Biol, San Marcos, TX USA. [Wozniak, Edward] Texas State Guard, Med Brigade, Uvalde, TX USA. [Boyle, William K.; Hargrove, Reid; Lopez, Job E.] Mississippi State Univ, Dept Biol Sci, Starkville, MS USA. [Wilder, Hannah K.; Lopez, Job E.] Baylor Coll Med, Dept Pediat, Natl Sch Trop Med, Houston, TX 77030 USA. RP Teel, PD (reprint author), Texas A&M AgriLife Res, Dept Entomol, College Stn, TX USA.; Lopez, JE (reprint author), Mississippi State Univ, Dept Biol Sci, Starkville, MS USA.; Lopez, JE (reprint author), Baylor Coll Med, Dept Pediat, Natl Sch Trop Med, Houston, TX 77030 USA. EM pteel@tamu.edu; job.lopez@bcm.edu FU United States Department of Agriculture-Agricultural Research Service [3094-32000-039-00D, 3094-32000-036-00D, 3094-32000-037-00D]; Texas A&M University Department of Entomology in the form of the Knipling-Bushland-SWAHRF Graduate Student Scholarship FX The research of AAPdL is funded through United States Department of Agriculture-Agricultural Research Service appropriated projects 3094-32000-039-00D, 3094-32000-036-00D, and 3094-32000-037-00D (http://www.ars.usda.gov/main/main.htm). Texas A&M University Department of Entomology provided support in the form of the Knipling-Bushland-SWAHRF Graduate Student Scholarship. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 69 TC 3 Z9 3 U1 5 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD FEB PY 2016 VL 10 IS 2 AR e0004383 DI 10.1371/journal.pntd.0004383 PG 19 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA DH1TH UT WOS:000372567300015 PM 26829327 ER PT J AU Hale, VC McDonnell, JJ Stewart, MK Solomon, DK Doolitte, J Ice, GG Pack, RT AF Hale, V. Cody McDonnell, Jeffrey J. Stewart, Michael K. Solomon, D. Kip Doolitte, Jim Ice, George G. Pack, Robert T. TI Effect of bedrock permeability on stream base flow mean transit time scaling relationships: 2. Process study of storage and release SO WATER RESOURCES RESEARCH LA English DT Article DE mean transit time; catchment storage; isotope hydrology; groundwater; surface water interaction; bedrock groundwater; groundwater dating ID RESIDENCE TIME; RUNOFF GENERATION; UNCHANNELED CATCHMENT; WATER STORAGE; GROUNDWATER CONTRIBUTION; CONTAMINANT TRANSPORT; ENVIRONMENTAL TRACERS; HEADWATER CATCHMENT; SHALLOW GROUNDWATER; HYDROLOGIC RESPONSE AB In Part 1 of this two-part series, Hale and McDonnell (2016) showed that bedrock permeability controlled base flow mean transit times (MTTs) and MTT scaling relations across two different catchment geologies in western Oregon. This paper presents a process-based investigation of storage and release in the more permeable catchments to explain the longer MTTs and (catchment) area-dependent scaling. Our field-based study includes hydrometric, MTT, and groundwater dating to better understand the role of subsurface catchment storage in setting base flow MTTs. We show that base flow MTTs were controlled by a mixture of water from discrete storage zones: (1) soil, (2) shallow hillslope bedrock, (3) deep hillslope bedrock, (4) surficial alluvial plain, and (5) suballuvial bedrock. We hypothesize that the relative contributions from each component change with catchment area. Our results indicate that the positive MTT-area scaling relationship observed in Part 1 is a result of older, longer flow path water from the suballuvial zone becoming a larger proportion of streamflow in a downstream direction (i.e., with increasing catchment area). Our work suggests that the subsurface permeability structure represents the most basic control on how subsurface water is stored and therefore is perhaps the best direct predictor of base flow MTT (i.e., better than previously derived morphometric-based predictors). Our discrete storage zone concept is a process explanation for the observed scaling behavior of Hale and McDonnell (2016), thereby linking patterns and processes at scales from 0.1 to 100 km(2). C1 [Hale, V. Cody] Nutter & Associates Inc, Athens, GA USA. [McDonnell, Jeffrey J.] Univ Saskatchewan, Global Inst Water Secur, Natl Hydrol Res Ctr, Saskatoon, SK, Canada. [McDonnell, Jeffrey J.] Univ Aberdeen, Northern Rivers Inst, Sch Geosci, Aberdeen, Scotland. [Stewart, Michael K.] Aquifer Dynam & GNS Sci, Lower Hutt, New Zealand. [Solomon, D. Kip] Univ Utah, Dept Geol & Geophys, Salt Lake City, UT 84112 USA. [Doolitte, Jim] Nat Resources Conservat Serv, USDA, Harrisburg, PA USA. [Ice, George G.] Natl Council Air & Stream Improvement Inc, Corvallis, OR USA. [Pack, Robert T.] Utah State Univ, Civil & Environm Engn, Logan, UT 84322 USA. RP Hale, VC (reprint author), Nutter & Associates Inc, Athens, GA USA. EM chale@nutterinc.com RI Solomon, Douglas/C-7951-2016 OI Solomon, Douglas/0000-0001-6370-7124 FU National Council for Air and Stream Improvement; AGU Horton Research Grant; University of Aberdeen's Northern Rivers Institute team FX The authors thank the National Council for Air and Stream Improvement and the AGU Horton Research Grant for support of this work. The CUAHSI Pathfinder grant program provided opportunity for Cody Hale to receive valuable feedback from the University of Aberdeen's Northern Rivers Institute team, led by Doerthe Tetzlaff and Chris Soulsby. Markus Hrachowitz, Christian Birkel, and Rene Capell are also thanked for their valuable feedback. Tina Garland is thanked for field assistance and isotope analysis assistance. Jakob Garvelmann from the Univeristy of Freiburg is thanked for his field efforts. Two anonymous reviewers are thanked for their valuable comments on an earlier draft of this manuscript. Data used for the analyses presented here will be made available upon request from the corresponding author. NR 112 TC 2 Z9 2 U1 10 U2 20 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD FEB PY 2016 VL 52 IS 2 BP 1375 EP 1397 DI 10.1002/2015WR017660 PG 23 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DH9LJ UT WOS:000373117300043 ER PT J AU Ghannam, K Nakai, T Paschalis, A Oishi, CA Kotani, A Igarashi, Y Kumagai, T Katul, GG AF Ghannam, Khaled Nakai, Taro Paschalis, Athanasios Oishi, Christopher A. Kotani, Ayumi Igarashi, Yasunori Kumagai, Tomo'omi Katul, Gabriel G. TI Persistence and memory timescales in root-zone soil moisture dynamics SO WATER RESOURCES RESEARCH LA English DT Article DE soil moisture; persistence; memory; climate; rainfall ID TEMPORAL VARIABILITY; STOCHASTIC-MODEL; TIME-SERIES; INTERANNUAL VARIATION; NORTHERN THAILAND; WATER-STRESS; SCALES; PRECIPITATION; RAINFALL; CLIMATE AB The memory timescale that characterizes root-zone soil moisture remains the dominant measure in seasonal forecasts of land-climate interactions. This memory is a quasi-deterministic timescale associated with the losses (e.g., evapotranspiration) from the soil column and is often interpreted as persistence in soil moisture states. Persistence, however, represents a distribution of time periods where soil moisture resides above or below some prescribed threshold and is therefore inherently probabilistic. Using multiple soil moisture data sets collected at high resolution (subhourly) across different biomes and climates, this paper explores the differences, underlying dynamics, and relative importance of memory and persistence timescales in root-zone soil moisture. A first-order Markov process, commonly used to interpret soil moisture fluctuations derived from climate simulations, is also used as a reference model. Persistence durations of soil moisture below the plant water-stress level (chosen as the threshold), and the temporal spectrum of upcrossings and downcrossings of this threshold, are compared to the memory timescale and spectrum of the full time series, respectively. The results indicate that despite the differences between meteorological drivers, the spectrum of threshold-crossings is similar across sites, and follows a unique relation with that of the full soil moisture series. The distribution of persistence times exhibits an approximate stretched exponential type and reflects a likelihood of exceeding the memory at all sites. However, the rainfall counterpart of these distributions shows that persistence of dry atmospheric periods is less likely at sites with long soil moisture memory. The cluster exponent, a measure of the density of threshold-crossings in a time frame, reveals that the clustering tendency in rainfall events (on-off switches) does not translate directly to clustering in soil moisture. This is particularly the case in climates where rainfall and evapotranspiration are out of phase, resulting in less ordered (more independent) persistence in soil moisture than in rainfall. C1 [Ghannam, Khaled; Katul, Gabriel G.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Nakai, Taro; Igarashi, Yasunori; Kumagai, Tomo'omi] Nagoya Univ, Hydrospher Atmospher Res Ctr, Nagoya, Aichi 4648601, Japan. [Nakai, Taro] Univ Alaska Fairbanks, Int Arctic Res Ctr, Fairbanks, AK USA. [Paschalis, Athanasios] Univ Southampton, Fac Engn & Environm, Southampton, Hants, England. [Oishi, Christopher A.] US Forest Serv, Coweeta Hydrol Lab, USDA, Otto, NC USA. [Kotani, Ayumi] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan. RP Ghannam, K (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM khaled.ghannam@duke.edu RI Kumagai, Tomo'omi/A-4791-2011; Nakai, Taro/B-7417-2008; Katul, Gabriel/A-7210-2008; Ghannam, Khaled/C-2334-2017 OI Nakai, Taro/0000-0002-6107-0614; Katul, Gabriel/0000-0001-9768-3693; Ghannam, Khaled/0000-0002-2542-6388 FU Swiss National Sciences Foundation [P2EZP2-152244]; Stavros Niarchos Foundation, through the SNSF Early Postdoc Mobility Fellowship; Ministry of Education, Culture, Sports, Science, and Technology-Japan (MEXT) FX The authors thank Amilcare Porporato for the helpful comments and suggestions that motivated the discussion and supporting information. Ghannam thanks Natsuko Yoshifuji for providing the soil moisture and meteorological data at Mae Moh forest. Katul and Ghannam acknowledge the National Science Foundation (NSF-CBET-103347 and NSF-EAR-1344703) and the U.S. Department of Energy (DOE) through the office of Biological and Environmental Research (BER) Terrestrial Ecosystem Science (TES) Program (DE-SC0006967 and DE-SC0011461). Paschalis acknowledges the financial support of the Swiss National Sciences Foundation (grant P2EZP2-152244) and the Stavros Niarchos Foundation, through the SNSF Early Postdoc Mobility Fellowship. Nakai, Igarashi, and Kumagai acknowledge the framework of the "Precise Impact Assessments on Climate Change'' of the Program for Risk Information on Climate Change (SOUSEI Program) supported by the Ministry of Education, Culture, Sports, Science, and Technology-Japan (MEXT). Additional information about the data sets used here can be obtained from the corresponding author at khaled.ghannam@duke.edu. NR 61 TC 5 Z9 5 U1 8 U2 12 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD FEB PY 2016 VL 52 IS 2 BP 1427 EP 1445 DI 10.1002/2015WR017983 PG 19 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DH9LJ UT WOS:000373117300046 ER PT J AU Ward, AS Schmadel, NM Wondzell, SM Harman, C Gooseff, MN Singha, K AF Ward, Adam S. Schmadel, Noah M. Wondzell, Steven M. Harman, Ciaran Gooseff, Michael N. Singha, Kamini TI Hydrogeomorphic controls on hyporheic and riparian transport in two headwater mountain streams during base flow recession SO WATER RESOURCES RESEARCH LA English DT Article DE solute transport; solute tracer; riparian hydrology; time-variable transit time distribution; hyporheic ID AMBIENT GROUNDWATER DISCHARGE; SUBSURFACE WATER EXCHANGE; TRANSIENT STORAGE; SOLUTE TRANSPORT; ZONE; SURFACE; CATCHMENTS; DYNAMICS; MODELS; OREGON AB Solute transport along riparian and hyporheic flow paths is broadly expected to respond to dynamic hydrologic forcing by streams, aquifers, and hillslopes. However, direct observation of these dynamic responses is lacking, as is the relative control of geologic setting as a control on responses to dynamic hydrologic forcing. We conducted a series of four stream solute tracer injections through base flow recession in each of two watersheds with contrasting valley morphology in the H.J. Andrews Experimental Forest, monitoring tracer concentrations in the stream and in a network of shallow riparian wells in each watershed. We found hyporheic mean arrival time, temporal variance, and fraction of stream water in the bedrock-constrained valley bottom and near large roughness elements in the wider valley bottom were not variable with discharge, suggesting minimal control by hydrologic forcing. Conversely, we observed increases in mean arrival time and temporal variance and decreasing fraction stream water with decreasing discharge near the hillslopes in the wider valley bottom. This may indicate changes in stream discharge and valley bottom hydrology control transport in less constrained locations. We detail five hydrogeomorphic responses to base flow recession to explain observed spatial and temporal patterns in the interactions between streams and their valley bottoms. Models able to account for the transition from geologically dominated processes in the near-stream subsurface to hydrologically dominated processes near the hillslope will be required to predict solute transport and fate in valley bottoms of headwater mountain streams. C1 [Ward, Adam S.; Schmadel, Noah M.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN USA. [Wondzell, Steven M.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Harman, Ciaran] Johns Hopkins Univ, Dept Geog & Environm Engn, Baltimore, MD 21218 USA. [Gooseff, Michael N.] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. [Gooseff, Michael N.] Univ Colorado, Dept Civil Environm & Architectural Engn, Boulder, CO 80309 USA. [Singha, Kamini] Colorado Sch Mines, Dept Geol & Geol Engn, Golden, CO 80401 USA. [Singha, Kamini] Colorado Sch Mines, Hydrol Sci & Engn Program, Golden, CO 80401 USA. RP Ward, AS (reprint author), Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN USA. EM adamward@indiana.edu RI Harman, Ciaran/A-7974-2013; OI Harman, Ciaran/0000-0002-3185-002X; Ward, Adam/0000-0002-6376-0061 FU National Science Foundation's (NSF's) Long-Term Ecological Research Program [DEB 1440409]; U.S. Forest Service Pacific Northwest Research Station; Oregon State University; NSF's Hydrologic Sciences program [EAR 0911435]; NSF [EAR 1417603]; National Science Foundation (NSF) [EAR 1331906] FX Data and facilities were provided by the H.J. Andrews Experimental Forest research program, funded by the National Science Foundation's (NSF's) Long-Term Ecological Research Program (DEB 1440409), U.S. Forest Service Pacific Northwest Research Station, and Oregon State University. Gooseff, Singha, and Ward were supported by the NSF's Hydrologic Sciences program, under grant EAR 0911435. Wondzell was supported by NSF grant EAR 1417603. Tools for solute tracer time series analyses were developed by Ward and others with support provided in part by the National Science Foundation (NSF) grant EAR 1331906 for the Critical Zone Observatory for Intensively Managed Landscapes (IML-CZO), a multiinstitutional collaborative effort. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation, U.S. Forest Service, or the H.J. Andrews Experimental Forest. Precipitation and discharge data are available from the H.J. Andrews Experimental Forest Data Catalog (http://andrewsforest.oregonstate.edu/). Topographic survey, in-stream specific conductance, and down-well specific conductance data are available upon request to the corresponding author. The authors declare no conflicts of interest. NR 80 TC 4 Z9 4 U1 5 U2 16 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD FEB PY 2016 VL 52 IS 2 BP 1479 EP 1497 DI 10.1002/2015WR018225 PG 19 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DH9LJ UT WOS:000373117300049 ER PT J AU Lakeh, AAB Farahmand, H Kloas, W Mirvaghefi, A Trubiroha, A Peterson, BC Wuertz, S AF Lakeh, Amir Abbas Bazyar Farahmand, Hamid Kloas, Werner Mirvaghefi, Alireza Trubiroha, Achim Peterson, Brian C. Wuertz, Sven TI Growth enhancement of rainbow trout (Oncorhynchus mykiss) by passive immunization against somatostatin-14 SO AQUACULTURE INTERNATIONAL LA English DT Article DE Growth hormone; Immunoglobulin Y; Insulin-like growth factor I; Rainbow trout; Somatostatin-14 ID CATFISH ICTALURUS-PUNCTATUS; MESSENGER-RNA EXPRESSION; I IGF-I; COHO SALMON; INHIBITS GROWTH; HORMONE GH; FISH; RECEPTOR; ENDOCRINE; SECRETION AB Juvenile rainbow trout (Oncorhynchus mykiss) were passively immunized by intraperitoneal immunization against somatostatin-14 (SS-14) using an antibody originating from egg-laying chicken (Gallus domesticus). Fish were immunized weekly (0, 7, 14, 21, 28, 35 days) with chicken egg yolk-derived immunoglobulin (IgY) against SS-14 (1: 25 IgY, 5 mg mL(-1)), and growth performance, feed utilization as well as plasma concentrations and mRNA levels of growth hormone (GH) and insulin-like growth factor I (IGF-I) were compared to the control group that received placebo immunization with PBS. Passive immunization significantly increased weight gain of treated fish (67.7 +/- 7.4 g) compared to the control group (40.1 +/- 2.0 g) after 35 days (p < 0.05). Feed conversion ratio (FCR) was significantly improved in the immunized fish (0.7 +/- 0.08) compared to control group (1.2 +/- 0.06) (p < 0.05). The concentrations of GH and IGF-I in the blood plasma showed no significant differences between the fish treated with anti-SS-14 and those of control during the treatment (p > 0.05). In both groups, GH levels decreased over the 35 days of the experiment (p < 0.05). However, IGF-I level during the period of treatment remained unchanged in both control and immunized fish with the anti-SS-14. Similarly, no changes were observed in pituitary GH and liver IGF-I mRNA levels between treatment and control at each sampling time (p > 0.05). There was no indication of a cumulative, long-lasting effect of repeated immunization on GH or IGF-I plasma concentrations or mRNA expression. The present study shows that a passive immunization of rainbow trout against SS-14 using a chicken egg yolk-derived SS-14 antibody could increase growth rate and improved FCR. C1 [Lakeh, Amir Abbas Bazyar; Kloas, Werner; Trubiroha, Achim; Wuertz, Sven] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecophysiol & Aquaculture, Muggelseedamm 310, D-12587 Berlin, Germany. [Lakeh, Amir Abbas Bazyar; Wuertz, Sven] Humboldt Univ, Albrecht Daniel Thaer Inst Agr & Hort Sci, Fac Life Sci, Invaliden Str 42, D-10115 Berlin, Germany. [Kloas, Werner] Humboldt Univ, Inst Biol, Fac Life Sci, Dept Endocrinol, Invaliden Str 42, D-10115 Berlin, Germany. [Farahmand, Hamid; Mirvaghefi, Alireza] Univ Tehran, Univ Coll Agr & Nat Resources, Fac Nat Resources, Dept Fishery & Environm, POB 31585-4314, Karaj, Iran. [Peterson, Brian C.] USDA, Warmwater Aquaculture Res Unit, POB 38, Stoneville, MS 38776 USA. RP Lakeh, AAB (reprint author), Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecophysiol & Aquaculture, Muggelseedamm 310, D-12587 Berlin, Germany.; Lakeh, AAB (reprint author), Humboldt Univ, Albrecht Daniel Thaer Inst Agr & Hort Sci, Fac Life Sci, Invaliden Str 42, D-10115 Berlin, Germany. EM bazyar@igb-berlin.de RI Wurtz, Sven/A-6550-2012 NR 41 TC 0 Z9 0 U1 4 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0967-6120 EI 1573-143X J9 AQUACULT INT JI Aquac. Int. PD FEB PY 2016 VL 24 IS 1 BP 11 EP 21 DI 10.1007/s10499-015-9905-8 PG 11 WC Fisheries SC Fisheries GA DH1NX UT WOS:000372553000002 ER PT J AU Reidy, JL Thompson, FR Amundson, C O'Donnell, L AF Reidy, Jennifer L. Thompson, Frank R., III Amundson, Courtney O'Donnell, Lisa TI Landscape and local effects on occupancy and densities of an endangered wood-warbler in an urbanizing landscape SO LANDSCAPE ECOLOGY LA English DT Article DE Canopy cover; Central Texas; Forest type; Golden-cheeked warbler; Setophaga chrysoparia; Urban land cover ID GOLDEN-CHEEKED WARBLERS; AVIAN POINT COUNTS; SETOPHAGA-CHRYSOPARIA; DETECTION PROBABILITY; BREEDING-SEASON; POPULATION-SIZE; MIXTURE-MODELS; NEST SURVIVAL; UNITED-STATES; BIRD SURVEYS AB Context Golden-cheeked warblers (Setophaga chrysoparia), an endangered wood-warbler, breed exclusively in woodlands co-dominated by Ashe juniper (Juniperus ashei) in central Texas. Their breeding range is becoming increasingly urbanized and habitat loss and fragmentation are a main threat to the species' viability. Objectives We investigated the effects of remotely sensed local habitat and landscape attributes on point occupancy and density of warblers in an urban preserve and produced a spatially explicit density map for the preserve using model-supported relationships. Methods We conducted 1507 point-count surveys during spring 2011-2014 across Balcones Canyonlands Preserve (BCP) to evaluate warbler habitat associations and predict density of males. We used hierarchical Bayesian models to estimate multiple components of detection probability and evaluate covariate effects on detection probability, point occupancy, and density. Results Point occupancy was positively related to landscape forest cover and local canopy cover; mean occupancy was 0.83. Density was influenced more by local than landscape factors. Density increased with greater amounts of juniper and mixed forest and decreased with more open edge. There was a weak negative relationship between density and landscape urban land cover. Conclusions Landscape composition and habitat structure were important determinants of warbler occupancy and density, and the large intact patches of juniper and mixed forest on BCP ([ 2100 ha) supported a high density of warblers. Increasing urbanization and fragmentation in the surrounding landscape will likely result in lower breeding density due to loss of juniper and mixed forest and increasing urban land cover and edge. C1 [Reidy, Jennifer L.] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Thompson, Frank R., III] Univ Missouri, US Forest Serv, No Res Stn, 202 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Amundson, Courtney] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. [O'Donnell, Lisa] City Austin, Austin Water Util, Wildland Conservat Div, 3621 Ranch Rd 620 South, Austin, TX 78738 USA. RP Reidy, JL (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM jennifer.reidy@gmail.com FU City of Austin; USDA Forest Service Northern Research Station FX We thank W. Dijak, U.S. Forest Service, and W. Simper, Travis County Natural Resources, for assisting with GIS analyses; P. Bullard, J. Edwardson, N. Flood, M. Frye, G. Geier, J. Halka, S. Stollery, and C. Weyenberg for assistance with data collection; the many BCP staff, partners, and volunteers, for collecting the territory mapping data; and G. Connette, C. Handel, R. Peak, J. Pierce, W. Reiner and two anonymous reviewers for comments on a draft of this manuscript. Funding for this research was provided by the City of Austin and USDA Forest Service Northern Research Station. Any use of trade, product, or firm names in this publication is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 75 TC 5 Z9 5 U1 6 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD FEB PY 2016 VL 31 IS 2 BP 365 EP 382 DI 10.1007/s10980-015-0250-0 PG 18 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DG8FR UT WOS:000372318900013 ER PT J AU Belaire, JA Westphal, LM Minor, ES AF Belaire, J. Amy Westphal, Lynne M. Minor, Emily S. TI Different social drivers, including perceptions of urban wildlife, explain the ecological resources in residential landscapes SO LANDSCAPE ECOLOGY LA English DT Article DE Yards; Residential landscapes; Urban birds; Social-ecological systems; Outdoor cat; Environmental behavior ID DOMESTIC GARDENS; SOCIOECONOMIC-STATUS; CONSERVATION; BIODIVERSITY; GREEN; ECOSYSTEMS; BEHAVIOR; BIRDS; CATS; MOTIVATIONS AB Context The conservation value of residential landscapes is becoming increasingly apparent in our urbanizing world. The ecological characteristics of residential areas are largely determined by the decisions of many individual "managers." In these complex socio-ecological systems, it is important to understand the factors that motivate human decision-making. Objectives Our first objective was to quantify wildlife resources and management activities in residential landscapes and compare vegetation in front and back yards. Our second objective was to test three hypotheses linked with variation in yards: socioeconomic characteristics, neighborhood design factors, and perceptions of neighborhood birds. Methods We conducted surveys of over 900 residents in 25 Chicago-area neighborhoods to examine the wildlife resources contained in front and back yards and the social factors associated with variation in yards. We used a multi-scalar approach to examine among-yard and among-neighborhood variation in residential landscapes. Results Results indicate that back yards contain more wildlife resources than front yards, including greater vegetation complexity, more plants with fruit/berries, and more plants intended to attract birds. Furthermore, different hypotheses explain variation in front and back yards. Perceptions of birds were most important in explaining variation in back yard vegetation and wildlife-friendly resources per parcel, while neighbors' yards and socioeconomic characteristics best explained front yard vegetation. Conclusions This study demonstrates the importance of back yards as an unexplored and underestimated resource for biodiversity. In addition, the results provide insight into the complex factors linked with yard decisions, notably that residents' connections with neighborhood birds appear to translate to on-the-ground actions. C1 [Belaire, J. Amy; Minor, Emily S.] Univ Illinois, Dept Biol Sci, 845 W Taylor,M-C 066, Chicago, IL 60607 USA. [Belaire, J. Amy] St Edwards Univ, Dept Nat Sci, Wild Basin Creat Res Ctr, 3001 S Congress Ave, Austin, TX 78704 USA. [Westphal, Lynne M.] US Forest Serv, No Res Stn, People & Their Environm Res Program, 1033 Univ Pl,Suite 360, Evanston, IL 60201 USA. RP Belaire, JA (reprint author), St Edwards Univ, Dept Nat Sci, Wild Basin Creat Res Ctr, 3001 S Congress Ave, Austin, TX 78704 USA. EM jbelaire@stedwards.edu FU National Science Foundation [DGE-0549245]; University of Illinois at Chicago's Department of Biological Sciences Elmer Hadley Graduate Research Award FX This study was based upon work supported by the National Science Foundation Grant DGE-0549245 and the University of Illinois at Chicago's Department of Biological Sciences Elmer Hadley Graduate Research Award. We thank C. Watkins for advice in social survey design and delivery, H. Gin for help with survey distribution, and L. Vonderlinden for verifying yard characteristics of respondents. We also thank the many residents of Cook County, Illinois who graciously participated in the survey. NR 64 TC 1 Z9 1 U1 19 U2 37 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD FEB PY 2016 VL 31 IS 2 BP 401 EP 413 DI 10.1007/s10980-015-0256-7 PG 13 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DG8FR UT WOS:000372318900015 ER PT J AU Alexandre, PM Stewart, SI Mockrin, MH Keuler, NS Syphard, AD Bar-Massada, A Clayton, MK Radeloff, VC AF Alexandre, Patricia M. Stewart, Susan I. Mockrin, Miranda H. Keuler, Nicholas S. Syphard, Alexandra D. Bar-Massada, Avi Clayton, Murray K. Radeloff, Volker C. TI The relative impacts of vegetation, topography and spatial arrangement on building loss to wildfires in case studies of California and Colorado SO LANDSCAPE ECOLOGY LA English DT Article DE WUI; Building loss; Wildfires; FRAGSTATS; Logistic regression; Best-subsets ID WILDLAND-URBAN INTERFACE; CHARACTERISTIC CLASSIFICATION-SYSTEM; GRASSLAND FIRES; FUEL BREAKS; WESTERN US; FORESTS; PATTERNS; CLIMATE; MANAGEMENT; SEVERITY AB Context Wildfires destroy thousands of buildings every year in the wildland urban interface. However, fire typically only destroys a fraction of the buildings within a given fire perimeter, suggesting more could be done to mitigate risk if we understood how to configure residential landscapes so that both people and buildings could survive fire. Objectives Our goal was to understand the relative importance of vegetation, topography and spatial arrangement of buildings on building loss, within the fire's landscape context. Methods We analyzed two fires: one in San Diego, CA and another in Boulder, CO. We analyzed Google Earth historical imagery to digitize buildings exposed to the fires, a geographic information system to measure some of the explanatory variables, and FRAGSTATS to quantify landscape metrics. Using logistic regression we conducted an exhaustive model search to select the best models. Results The type of variables that were important varied across communities. We found complex spatial effects and no single model explained building loss everywhere, but topography and the spatial arrangement of buildings explained most of the variability in building losses. Vegetation connectivity was more important than vegetation type. Conclusions Location and spatial arrangement of buildings affect which buildings burn in a wildfire, which is important for urban planning, building siting, landscape design of future development, and to target fire prevention, fuel reduction, and homeowner education efforts in existing communities. Landscape context of buildings and communities is an important aspect of building loss, and if taken into consideration, could help communities adapt to fire. C1 [Alexandre, Patricia M.; Stewart, Susan I.; Clayton, Murray K.; Radeloff, Volker C.] Univ Wisconsin, Dept Forest & Wildlife Ecol, SILVIS Lab, 1630 Linden Dr, Madison, WI 53706 USA. [Mockrin, Miranda H.] US Forest Serv, Rocky Mt Res Stn, USDA, 240 W Prospect Rd, Ft Collins, CO 80526 USA. [Keuler, Nicholas S.; Clayton, Murray K.] Univ Wisconsin, Dept Stat, 1300 Univ Ave, Madison, WI 53706 USA. [Syphard, Alexandra D.] Conservat Biol Inst, 10423 Sierra Vista Ave, La Mesa, CA 91941 USA. [Bar-Massada, Avi] Univ Haifa, Dept Biol & Environm, IL-36006 Qiryat Tivon, Israel. RP Alexandre, PM (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, SILVIS Lab, 1630 Linden Dr, Madison, WI 53706 USA. EM patmalico@gmail.com RI Alexandre, Patricia/I-6354-2015 OI Alexandre, Patricia/0000-0003-1242-1710 FU Rocky Mountain Research Station; Northern Research Station of the USDA Forest Service; Fulbright Exchange program fellowship; Foundation for Science and Technology (FCT-Portugal) - POPH-QREN-Tipology 4.1-Advanced formation - European Social fund [SFRH/BD/92960/2013]; MEC National Fund FX This work was supported by a research joint venture agreement with the Rocky Mountain Research Station and Northern Research Station of the USDA Forest Service, and by a Fulbright Exchange program fellowship awarded to Patricia Alexandre, and by a Ph.D. fellowship provided by the Foundation for Science and Technology to Patricia Alexandre in 2014 (FCT-Portugal-reference: SFRH/BD/92960/2013, financed by POPH-QREN-Tipology 4.1-Advanced formation funded by the European Social fund and by the MEC National Fund). Fulbright and FCT had no involvement in the study design, collection, analysis, and interpretation of the results or in the decision to publish. Forest Service scientists were involved in the study design, interpretation of the results and decision to publish. LANDFIRE data were provided by the U.S. Geological Survey Earth Resources Observation Systems (EROS) Data Center. We thank J. Jenness for his help with the Topographic Position Index tool extension for ArcGis, D. Helmers and M. Beighley for their advice, J. Orestes and T. Henriques for support with glmulti R package, and C. Frederick and S. Roberts for help with data collection. Three anonymous reviewers provided valuable feedback, which greatly improved our manuscript, and we thank them for their suggestions. NR 76 TC 5 Z9 5 U1 10 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD FEB PY 2016 VL 31 IS 2 BP 415 EP 430 DI 10.1007/s10980-015-0257-6 PG 16 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DG8FR UT WOS:000372318900016 ER PT J AU McKeown, NM Hruby, A Landberg, R Herrington, DM Lichtenstein, AH AF McKeown, Nicola M. Hruby, Adela Landberg, Rikard Herrington, David M. Lichtenstein, Alice H. TI Plasma alkylresorcinols, biomarkers of whole-grain intake, are not associated with progression of coronary artery atherosclerosis in postmenopausal women with coronary artery disease SO PUBLIC HEALTH NUTRITION LA English DT Article DE Alkylresorcinols; Whole grains; Biomarkers; Coronary artery disease ID FOOD-FREQUENCY QUESTIONNAIRE; CEREAL FIBER INTAKE; RYE INTAKE; CARDIOVASCULAR-DISEASE; REDUCED PROGRESSION; MEASUREMENT ERROR; HEART-DISEASE; WHEAT; REPRODUCIBILITY; HEALTH AB Objective The objective of the present study was to examine the relationship between plasma alkyresorcinol (AR) concentrations, which are biomarkers of whole-grain intake, and atherosclerotic progression over 3 years in postmenopausal women with coronary artery disease. Design Plasma AR concentrations were measured by a validated GC-MS method in fasting plasma samples. Atherosclerosis progression was assessed using change in mean minimal coronary artery diameter (MCAD) and percentage diameter stenosis (%ST), based on mean proximal vessel diameter across up to ten coronary segments. Dietary intake was estimated using a 126-item interviewer-administered FFQ. Setting A prospective study of postmenopausal women participating in the Estrogen Replacement and Atherosclerosis trial. Subjects For the analysis of plasma AR concentrations and atherosclerotic progression, plasma samples and follow-up data on angiography were available for 182 women. Results Mean whole-grain intake was 96 (se 06) servings per week. After multivariate adjustment, no significant associations were observed between plasma AR concentrations and change in mean MCAD or progression of %ST. Plasma AR concentrations were significantly correlated with dietary whole grains (r=035, P<0001), cereal fibre (r=033, P<0001), bran (r=015, P=005), total fibre (r=022, P=0003) and legume fibre (r=015, P=004), but not refined grains, fruit fibre or vegetable fibre. Conclusions Plasma AR concentrations were not significantly associated with coronary artery progression over a 3-year period in postmenopausal women with coronary artery disease. A moderate association was observed between plasma AR concentrations and dietary whole grains and cereal fibre, suggesting it may be a useful biomarker in observational studies. C1 [McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, 711 Washington St,9th Floor, Boston, MA 02111 USA. [Hruby, Adela] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Landberg, Rikard] Swedish Univ Agr Sci, Bioctr, Dept Food Sci, Uppsala, Sweden. [Landberg, Rikard] Karolinska Inst, Inst Environm Med, Nutr Epidemiol Unit, S-10401 Stockholm, Sweden. [Herrington, David M.] Wake Forest Baptist Med Ctr, Winston Salem, NC USA. [Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. RP McKeown, NM (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, 711 Washington St,9th Floor, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU National Heart, Lung, and Blood Institute [U01 HL-45488]; General Mills Bell Institute of Health and Nutrition FX Financial support: This work was supported by the National Heart, Lung, and Blood Institute (grant number U01 HL-45488). General Mills Bell Institute of Health and Nutrition provided funding to support the analysis of AR in plasma samples. NR 39 TC 1 Z9 1 U1 3 U2 7 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD FEB PY 2016 VL 19 IS 2 BP 326 EP 331 DI 10.1017/S1368980015001123 PG 6 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA DH2TR UT WOS:000372639900014 PM 25903091 ER PT J AU Faybishenko, B Hubbard, S Brodie, E Nico, P Molz, F Hunt, A Pachepsky, Y AF Faybishenko, Boris Hubbard, Susan Brodie, Eoin Nico, Peter Molz, Fred Hunt, Allen Pachepsky, Yakov TI Preface to the Special Issue of Vadose Zone Journal on Soil as Complex Systems SO VADOSE ZONE JOURNAL LA English DT Editorial Material C1 [Faybishenko, Boris] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, 1 Cyclotron Rd,MS 84-171, Berkeley, CA 94720 USA. [Hubbard, Susan] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, 1 Cyclotron Rd,MS 90-116, Berkeley, CA 94720 USA. [Brodie, Eoin] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Ecol, 1 Cyclotron Rd,MS70A-3317, Berkeley, CA 94720 USA. [Nico, Peter] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, 90R1116,1 Cyclotron Rd, Berkeley, CA 94720 USA. [Molz, Fred] Clemson Univ, Environm Engn & Earth Sci, Rich Lab, 342 Comp Court, Anderson, SC 29625 USA. [Hunt, Allen] Wright State Univ, Phys & Earth & Environm Sci, 3640 Colonel Glenn Hwy, Dayton, OH 45435 USA. [Pachepsky, Yakov] USDA ARS, Beltsville Agr Res Ctr, 10300 Baltimore Ave Bldg 173, Beltsville, MD 20705 USA. RP Faybishenko, B (reprint author), Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, 1 Cyclotron Rd,MS 84-171, Berkeley, CA 94720 USA. EM bfayb@lbl.gov RI Hubbard, Susan/E-9508-2010; Brodie, Eoin/A-7853-2008; Nico, Peter/F-6997-2010; Faybishenko, Boris/G-3363-2015; OI Brodie, Eoin/0000-0002-8453-8435; Nico, Peter/0000-0002-4180-9397; Faybishenko, Boris/0000-0003-0085-8499; Pachepsky, Yakov/0000-0003-0232-6090 NR 12 TC 0 Z9 0 U1 3 U2 8 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD FEB PY 2016 VL 15 IS 2 DI 10.2136/vzj2016.01.0005 PG 3 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DH5EN UT WOS:000372808400013 ER PT J AU Yakubova, G Kavetskiy, A Prior, SA Torbert, HA AF Yakubova, Galina Kavetskiy, Aleksandr Prior, Stephen A. Torbert, H. Allen TI Benchmarking the Inelastic Neutron Scattering Soil Carbon Method SO VADOSE ZONE JOURNAL LA English DT Article; Proceedings Paper CT 1st Complex Soil Systems Conference CY SEP 03-05, 2014 CL Lawrence Berkeley Natl Lab, Berkeley, CA SP SSSA Bouyoucos Funds, Berkeley Lab, USDOE, MoBio Lab Inc HO Lawrence Berkeley Natl Lab ID SYSTEM; SPECTROSCOPY; IMPACT AB The herein described inelastic neutron scattering (INS) method of measuring soil carbon was based on a new procedure for extracting the net carbon signal (NCS) from the measured gamma spectra and determination of the average carbon weight percent in the upper similar to 8-cm soil layer (AvgCw% 8). The NCS extraction utilized the net-INS spectrum, which was the difference between the INS and thermal neutron capture (TNC) spectra and the net-INS system background spectrum. The proportionality between NCS and AvgCw% 8 for any shape of soil carbon depth distribution was demonstrated theoretically. The theoretical model for NCS calculations accounted for carbon depth distribution and neutron and gamma ray propagation laws in our analysis; previous model results were verified by comparison with a Monte Carlo simulation using Geant4. The experimental results confirmed the identified proportionality. The mobile INS system was calibrated using pits filled with synthetic soil; this calibration was used for AvgCw% 8 determinations in INS field measurements. The AvgCw% 8 was also determined by the dry combustion method. Benchmarking the soil carbon determination by INS demonstrated results that coincided with dry combustion technique (DCT) results (within experimental error limits). Given the agreement between these methods, the described INS measurement system can be recommended as a reliable alternative means for measuring soil carbon. C1 [Yakubova, Galina; Kavetskiy, Aleksandr; Prior, Stephen A.; Torbert, H. Allen] USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. RP Yakubova, G (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. EM galina.yakubova@ars.usda.gov NR 21 TC 3 Z9 3 U1 3 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD FEB PY 2016 VL 15 IS 2 DI 10.2136/vzj2015.04.0056 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DH5EN UT WOS:000372808400002 ER PT J AU Li, H Deng, X Dolloff, CA Smith, EP AF Li, H. Deng, X. Dolloff, C. A. Smith, E. P. TI Bivariate functional data clustering: grouping streams based on a varying coefficient model of the stream water and air temperature relationship SO ENVIRONMETRICS LA English DT Article DE bivariate functional data; variogram; varying coefficient model; water-air relationship; weighted distance ID LONGITUDINAL DATA; REGRESSION-MODEL; QUALITY; NETWORKS; CLIMATE; SYSTEM; TROUT AB A novel clustering method for bivariate functional data is proposed to group streams based on their water-air temperature relationship. A distance measure is developed for bivariate curves by using a time-varying coefficient model and a weighting scheme. This distance is also adjusted by spatial correlation of streams via the variogram. Therefore, the proposed distance not only measures the difference among the streams with respect to their water-air temperature relationship but also accounts for spatial correlation among the streams. The proposed clustering method is applied to 62 streams in Southeast US that have paired air-water temperature measured over a ten-month period. The results show that streams in the same cluster reflect common characteristics such as solar radiation, percent forest and elevation. Copyright (C) 2015 John Wiley & Sons, Ltd C1 [Li, H.; Deng, X.; Smith, E. P.] Virginia Tech, Dept Stat, 406A Hutcheson Hall, Blacksburg, VA 24061 USA. [Dolloff, C. A.] US Forest Serv, USDA, Southern Res Stn, Dept Fish & Wildlife Conservat, Blacksburg, VA 24060 USA. RP Smith, EP (reprint author), Virginia Tech, Dept Stat, 406A Hutcheson Hall, Blacksburg, VA 24061 USA. EM epsmith@vt.edu FU USFS Southern Research Station [JV 2010 11330140-137] FX We thank Joe Cline for data collection and management, and the USFS Southern Research Station for support under JV 2010 11330140-137. Helpful comments from Hongxiao Zhu on the manuscript were appreciated. NR 39 TC 1 Z9 1 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1180-4009 EI 1099-095X J9 ENVIRONMETRICS JI Environmetrics PD FEB PY 2016 VL 27 IS 1 BP 15 EP 26 DI 10.1002/env.2370 PG 12 WC Environmental Sciences; Mathematics, Interdisciplinary Applications; Statistics & Probability SC Environmental Sciences & Ecology; Mathematics GA DG4FY UT WOS:000372028200002 ER PT J AU Elias, E Rodriguez, H Srivastava, P Dougherty, M James, D Smith, R AF Elias, Emile Rodriguez, Hugo Srivastava, Puneet Dougherty, Mark James, Darren Smith, Ryann TI Impacts of Forest to Urban Land Conversion and ENSO Phase on Water Quality of a Public Water Supply Reservoir SO FORESTS LA English DT Article DE reservoir model; urbanization; deforestation; drinking water treatment; total organic carbon; disinfection byproducts; ENSO ID NINO SOUTHERN-OSCILLATION; CLIMATE VARIABILITY; LOAD; QUANTIFICATION; PRECIPITATION; NUTRIENT; MODEL AB We used coupled watershed and reservoir models to evaluate the impacts of deforestation and l Nino Southern Oscillation (ENSO) phase on drinking water quality. Source water total organic carbon (TOC) is especially important due to the potential for production of carcinogenic disinfection byproducts (DBPs). The Environmental Fluid Dynamics Code (EFDC) reservoir model is used to evaluate the difference between daily pre- and post- urbanization nutrients and TOC concentration. Post-disturbance (future) reservoir total nitrogen (TN), total phosphorus (TP), TOC and chlorophyll-a concentrations were found to be higher than pre-urbanization (base) concentrations (p < 0.05). Predicted future median TOC concentration was 1.1 mgL(-1) (41% higher than base TOC concentration) at the source water intake. Simulations show that prior to urbanization, additional water treatment was necessary on 47% of the days between May and October. However, following simulated urbanization, additional drinking water treatment might be continuously necessary between May and October. One of six ENSO indices is weakly negatively correlated with the measured reservoir TOC indicating there may be higher TOC concentrations in times of lower streamflow (La Nina). There is a positive significant correlation between simulated TN and TP concentrations with ENSO suggesting higher concentrations during El Nino. C1 [Elias, Emile; James, Darren] New Mexico State Univ, USDA ARS, Wooton Hall, Las Cruces, NM 88003 USA. [Rodriguez, Hugo] TetraTech Inc, 2110 Powers Ferry Rd, Atlanta, GA 30326 USA. [Srivastava, Puneet; Dougherty, Mark] Auburn Univ, 206 Tom Corley Bldg, Auburn, AL 36832 USA. [Smith, Ryann] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Elias, E (reprint author), New Mexico State Univ, USDA ARS, Wooton Hall, Las Cruces, NM 88003 USA. EM eliaseh@nmsu.edu; hugo.rodriguez@tetratech.com; srivapu@auburn.edu; doughmp@auburn.edu; darren.k.james@gmail.com; rxsmith3@nmsu.edu FU Center for Forest Sustainability, Auburn University FX We thank Jamie Childers (TetraTech, Atlanta, GA), Amy Gill, (Alabama United States Geological Survey), and Tony Fisher (Mobile Area Water and Sewer Systems) for their assistance in this research. We also acknowledge and sincerely thank the Center for Forest Sustainability, Auburn University, for funding this research. NR 58 TC 0 Z9 0 U1 9 U2 20 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD FEB PY 2016 VL 7 IS 2 AR 29 DI 10.3390/f7020029 PG 17 WC Forestry SC Forestry GA DG2KW UT WOS:000371896900020 ER PT J AU Overby, ST Hart, SC AF Overby, Steven T. Hart, Stephen C. TI Short-Term Belowground Responses to Thinning and Burning Treatments in Southwestern Ponderosa Pine Forests of the USA SO FORESTS LA English DT Article DE fuel treatments; nitrification; nitrogen mineralization; phospholipid fatty acids; soil enzymes ID ALTERS NITROGEN TRANSFORMATIONS; FIRE SURROGATE TREATMENTS; FUEL-REDUCTION TREATMENTS; SOIL MICROBIAL COMMUNITY; VEGETATION STRUCTURE; UNITED-STATES; RESTORATION TREATMENTS; BUNCHGRASS ECOSYSTEM; LIPID PHOSPHATE; ENZYME-ACTIVITY AB Microbial-mediated decomposition and nutrient mineralization are major drivers of forest productivity. As landscape-scale fuel reduction treatments are being implemented throughout the fire-prone western United States of America, it is important to evaluate operationally how these wildfire mitigation treatments alter belowground processes. We quantified these important belowground components before and after management-applied fuel treatments of thinning alone, thinning combined with prescribed fire, and prescribed fire in ponderosa pine (Pinus ponderosa) stands at the Southwest Plateau, Fire and Fire Surrogate site, Arizona. Fuel treatments did not alter pH, total carbon and nitrogen (N) concentrations, or base cations of the forest floor (O horizon) or mineral soil (0-5 cm) during this 2-year study. In situ rates of net N mineralization and nitrification in the surface mineral soil (0-15 cm) increased 6 months after thinning with prescribed fire treatments; thinning only resulted in net N immobilization. The rates returned to pre-treatment levels after one year. Based on phospholipid fatty acid composition, microbial communities in treated areas were similar to untreated areas (control) in the surface organic horizon and mineral soil (0-5 cm) after treatments. Soil potential enzyme activities were not significantly altered by any of the three fuel treatments. Our results suggest that a variety of one-time alternative fuel treatments can reduce fire hazard without degrading soil fertility. C1 [Overby, Steven T.] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Overby, Steven T.; Hart, Stephen C.] Univ Arizona, Sch Forestry, Flagstaff, AZ 86011 USA. [Hart, Stephen C.] Univ Calif, Sierra Nevada Res Inst, Life & Environm Sci, Merced, CA 95343 USA. RP Overby, ST (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA.; Overby, ST (reprint author), Univ Arizona, Sch Forestry, Flagstaff, AZ 86011 USA. EM soverby@fs.fed.us; shart4@ucmerced.edu FU U.S. Joint Fire Science Program; Rocky Mountain Research Station [06-JV-11221615-228] FX This is Contribution Number 208 of the National Fire and Fire Surrogate Project (FFS), funded by the U.S. Joint Fire Science Program. Additional funding was provided by the Rocky Mountain Research Station (Research Joint Venture 06-JV-11221615-228). We thank Dana Erickson for field and laboratory assistance. We are also grateful to Ralph Boerner, Ohio State University, for technical assistance and leadership of the soils group of the Fire-Fire Surrogate network and Carl Edminster (RMRS), the Southwestern Plateau FFS site manager. NR 80 TC 2 Z9 2 U1 4 U2 14 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD FEB PY 2016 VL 7 IS 2 AR 45 DI 10.3390/f7020045 PG 19 WC Forestry SC Forestry GA DG2KW UT WOS:000371896900013 ER PT J AU Upadhyaya, HD Wang, YH Sastry, DVSSR Dwivedi, SL Prasad, PVV Burrell, AM Klein, RR Morris, GP Klein, PE AF Upadhyaya, Hari D. Wang, Yi-Hong Sastry, Dintyala V. S. S. R. Dwivedi, Sangam L. Prasad, P. V. Vara Burrell, A. Millie Klein, Robert R. Morris, Geoffrey P. Klein, Patricia E. TI Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions SO GENOME LA English DT Article DE sorghum; association mapping; SNP; early-season cold tolerance ID MINI-CORE COLLECTION; GENOME-WIDE ASSOCIATION; SEASON COLD TOLERANCE; LINKAGE DISEQUILIBRIUM; COMPLEX TRAITS; GERMPLASM; BICOLOR; MODEL; PLANT; REVEALS AB Sorghum is one of the world's most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum's growing season and increases yield in temperate regions. However, sorghum's sensitivity to low soil temperatures adversely impacts seed germination. In this study, we evaluated the 242 accessions of the ICRISAT sorghum mini core collection for seed germination and seedling vigor at 12 degrees C as a measure of cold tolerance. Genome-wide association analysis was performed with approximately 162 177 single nucleotide polymorphism markers. Only one marker locus (Locus 7-2) was significantly associated with low-temperature germination and none with vigor. The linkage of Locus 7-2 to low-temperature germination was supported by four lines of evidence: strong association in three independent experiments, co-localization with previously mapped cold tolerance quantitative trait loci (QTL) in sorghum, a candidate gene that increases cold tolerance and germination rate when its wheat homolog is overexpressed in tobacco, and its syntenic region in rice co-localized with two cold tolerance QTL in rice. This locus may be useful in developing tools for molecular breeding of sorghums with improved low-temperature germinability. C1 [Upadhyaya, Hari D.; Sastry, Dintyala V. S. S. R.; Dwivedi, Sangam L.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. [Upadhyaya, Hari D.; Prasad, P. V. Vara; Morris, Geoffrey P.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Upadhyaya, Hari D.] Univ Western Australia, Inst Agr, Crawley, WA 6009, Australia. [Wang, Yi-Hong] Univ Louisiana Lafayette, Dept Biol, Lafayette, LA 70504 USA. [Burrell, A. Millie; Klein, Patricia E.] Texas A&M Univ, Dept Hort, College Stn, TX 77843 USA. [Burrell, A. Millie; Klein, Patricia E.] Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA. [Klein, Robert R.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Wang, YH (reprint author), Univ Louisiana Lafayette, Dept Biol, Lafayette, LA 70504 USA. EM yxw9887@louisiana.edu RI Updhayaya, Hari/C-4858-2014 OI Updhayaya, Hari/0000-0002-5166-6844 FU International Crops Research Institute for the Semi-Arid Tropics; University of Louisiana at Lafayette FX The study is supported in part by the International Crops Research Institute for the Semi-Arid Tropics and the University of Louisiana at Lafayette. This work has been undertaken as part of the CGIAR Research Program on Dryland Cereals. We thank the editor and the reviewers whose suggestions have greatly improved the manuscript. NR 37 TC 3 Z9 3 U1 3 U2 18 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD FEB PY 2016 VL 59 IS 2 BP 137 EP 145 DI 10.1139/gen-2015-0122 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DG6ML UT WOS:000372199000006 PM 26758024 ER PT J AU Erginbas-Orakci, G Poole, G Nicol, JM Paulitz, T Dababat, AA Campbell, K AF Erginbas-Orakci, Gul Poole, Grant Nicol, Julie M. Paulitz, Timothy Dababat, Abdelfattah A. Campbell, Kimberley TI Assessment of inoculation methods to identify resistance to Fusarium crown rot in wheat SO JOURNAL OF PLANT DISEASES AND PROTECTION LA English DT Article DE Fusarium culmorum; Fusarium pseudograminearum; Screening methods; Triticum aestivum ID GRAMINEARUM GROUP-1; PACIFIC-NORTHWEST; EASTERN AUSTRALIA; ROOT DISEASES; FOOT ROT; PSEUDOGRAMINEARUM; BARLEY; SEEDLINGS; IDENTIFICATION; CULMORUM AB Crown rot, caused by Fusarium culmorum and Fusarium pseudograminearum, is one of the most pervasive diseases of wheat throughout the world. Fusarium culmorum is the most prevalent causal agent in Turkey while F. pseudograminearum is the most predominant in the USA. Consistent and reliable screening methods are required to accelerate the identification and development of wheat cultivars for resistance to Fusarium crown rot in breeding programs. A multifactor experiment with seven replicates was established investigating three different inoculation techniques (seedling dip, stem base droplet and colonized grain) using two pathogenic isolates of F. culmorum in Turkey and Fusarium pseudograminearum in the USA, respectively, against known moderately resistant and susceptible wheat cultivars under controlled greenhouse conditions. Plants were harvested and evaluated for crown rot severity using a 0-10 rating scale. Results indicated that disease severity was greater in seedling dip, compared to colonized grain, and stem base inoculation (in decreasing order of severity), respectively. However, the colonized grain method produced an adequate level of severity and consistent cultivar ranking in both experiments. Results showed significant cultivar 9 inoculation method interactions, and the two species of Fusarium were considered to be virulent. C1 [Erginbas-Orakci, Gul; Nicol, Julie M.; Dababat, Abdelfattah A.] Int Maize & Wheat Improvement Ctr, Global Wheat Program, PK 39 Emek, Ankara, Turkey. [Poole, Grant] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Paulitz, Timothy] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Campbell, Kimberley] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. RP Erginbas-Orakci, G (reprint author), Int Maize & Wheat Improvement Ctr, Global Wheat Program, PK 39 Emek, Ankara, Turkey. EM g.erginbas@cgiar.org OI Paulitz, Timothy/0000-0002-8885-3803 FU CIM-MYT (International Maize and Wheat Improvement Center); Washington Grain Commission; Plant Growth Facility at Washington State University FX The authors would like to thank the Transitional Zone Agriculture Research Institute of Eskisehir, Turkey, which provided technical support. Support was also provided by the CIM-MYT (International Maize and Wheat Improvement Center) for travel exchanges of the first and second authors through the United States Agency for International Development (US-AID) collaboration, as part of the global initiative "Integrated Management of Soil-Borne Pathogens and Pests for Resource-Poor Farmers of Rainfed Wheat.'' In the USA, the authors would like to thank the Washington Grain Commission and the Plant Growth Facility at Washington State University for providing funding and technical support. NR 33 TC 0 Z9 1 U1 5 U2 10 PU EUGEN ULMER GMBH CO PI STUTTGART PA POSTFACH 700561 WOLLGRASWEG 41, D-70599 STUTTGART, GERMANY SN 1861-3829 EI 1861-3837 J9 J PLANT DIS PROTECT JI J. Plant Dis. Prot. PD FEB PY 2016 VL 123 IS 1 BP 19 EP 27 DI 10.1007/s41348-016-0001-8 PG 9 WC Agriculture, Multidisciplinary; Plant Sciences SC Agriculture; Plant Sciences GA DG7RD UT WOS:000372280500003 ER PT J AU Christensen, SA Huffaker, A Hunter, CT Alborn, HT Schmelz, EA AF Christensen, Shawn A. Huffaker, Alisa Hunter, Charles T. Alborn, Hans T. Schmelz, Eric A. TI A maize death acid, 10-oxo-11-phytoenoic acid, is the predominant cyclopentenone signal present during multiple stress and developmental conditions SO PLANT SIGNALING & BEHAVIOR LA English DT Article DE Cyclopentenone; defense; maize; oxylipin; Ten-oxo-11-phytoenoic acid; 9-lipoxygenase; 12-oxophytodienoic acid ID JASMONIC ACID; LINOLENIC ACIDS; CELL-DEATH; DEFENSE; RESPONSES; BIOSYNTHESIS; LIPOXYGENASE; PHYTOALEXINS; ARABIDOPSIS; DISRUPTION AB Recently we investigated the function of the 9-lipoxygenase (LOX) derived cyclopentenones 10-oxo-11-phytoenoic acid (10-OPEA) and 10-oxo-11,15-phytodienoic acid (10-OPDA) and identified their C-14 and C-12 derivatives. 10-OPEA accumulation is elicited by fungal and insect attack and acts as a strong inhibitor of microbial and herbivore growth. Although structurally similar, comparative analyses between 10-OPEA and its 13-LOX analog 12-oxo-phytodienoic acid (12-OPDA) demonstrate specificity in transcript accumulation linked to detoxification, secondary metabolism, jasmonate regulation, and protease inhibition. As a potent cell death signal, 10-OPEA activates cysteine protease activity leading to ion leakage and apoptotic-like DNA fragmentation. In this study we further elucidate the distribution, abundance, and functional roles of 10-OPEA, 10-OPDA, and 12-OPDA, in diverse organs under pathogen- and insect-related stress. C1 [Christensen, Shawn A.; Hunter, Charles T.; Alborn, Hans T.] Univ Florida, USDA ARS, Chem Res Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL 32611 USA. [Huffaker, Alisa; Schmelz, Eric A.] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA. RP Christensen, SA (reprint author), Univ Florida, USDA ARS, Chem Res Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL 32611 USA. EM shawn.christensen@ars.usda.gov NR 27 TC 1 Z9 1 U1 2 U2 9 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1559-2316 EI 1559-2324 J9 PLANT SIGNAL BEHAV JI Plant Signal. Behav. PD FEB 1 PY 2016 VL 11 IS 2 AR e1120395 DI 10.1080/15592324.2015.1120395 PG 5 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DG4EL UT WOS:000372024000020 PM 26669723 ER PT J AU Cheng, T Yang, ZW Inoue, Y Zhu, Y Cao, WX AF Cheng, Tao Yang, Zhengwei Inoue, Yoshio Zhu, Yan Cao, Weixing TI Preface: Recent Advances in Remote Sensing for Crop Growth Monitoring SO REMOTE SENSING LA English DT Editorial Material DE crop status; crop monitoring; crop mapping; canopy reflectance; spectral; optical; SAR; phenology ID VEGETATION INDEXES; TIME-SERIES; MODIS DATA; SAR DATA; AREA; IMAGES; WHEAT AB This Special Issue gathers sixteen papers focusing on applying various remote sensing techniques to crop growth monitoring. The studies span observations from multiple scales, a combination of model simulations and experimental measurements, and a range of topics on crop monitoring and mapping. This preface provides a brief overview of the contributed papers. C1 [Cheng, Tao; Zhu, Yan; Cao, Weixing] Nanjing Agr Univ, Natl Engn & Technol Ctr Informat Agr, Jiangsu Key Lab Informat Agr, Nanjing 210095, Jiangsu, Peoples R China. [Cheng, Tao; Zhu, Yan; Cao, Weixing] Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing 210095, Jiangsu, Peoples R China. [Yang, Zhengwei] Natl Agr Stat Serv, USDA, Div Res & Dev, 3251 Old Iee Highway,Room 305, Fairfax, VA 22030 USA. [Inoue, Yoshio] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan. RP Zhu, Y (reprint author), Nanjing Agr Univ, Natl Engn & Technol Ctr Informat Agr, Jiangsu Key Lab Informat Agr, Nanjing 210095, Jiangsu, Peoples R China.; Zhu, Y (reprint author), Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing 210095, Jiangsu, Peoples R China. EM tcheng@njau.edu.cn; zhengwei.yang@nass.usda.gov; yinoue@affrc.go.jp; yanzhu@njau.edu.cn; caow@njau.edu.cn RI Cheng, Tao/B-4807-2010 OI Cheng, Tao/0000-0002-4184-0730 NR 16 TC 0 Z9 0 U1 10 U2 24 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD FEB PY 2016 VL 8 IS 2 AR 116 DI 10.3390/rs8020116 PG 3 WC Remote Sensing SC Remote Sensing GA DG2LO UT WOS:000371898800071 ER PT J AU Ruiz, O Castillo, Y Arzola, C Burrola, E Salinas, J Corral, A Hume, ME Murillo, M Itza, M AF Ruiz, Oscar Castillo, Yamicela Arzola, Claudio Burrola, Eduviges Salinas, Jaime Corral, Agustin Hume, Michael E. Murillo, Manuel Itza, Mateo TI Effects of Candida norvegensis Live Cells on In vitro Oat Straw Rumen Fermentation SO ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES LA English DT Article DE Rumen; Fermentation; Yeast; Oat Straw; Methane ID CULTURE SACCHAROMYCES-CEREVISIAE; RUMINAL MICROBIAL-METABOLISM; YEAST CULTURE; DAIRY-COWS; SUPPLEMENTATION; GROWTH; IDENTIFICATION; DEGRADABILITY; DEGRADATION; ENUMERATION AB This study evaluated the effect of Candida norvegensis (C. norvegensis) viable yeast culture on in vitro ruminal fermentation of oat straw. Ruminal fluid was mixed with buffer solution (1: 2) and anaerobically incubated with or without yeast at 39 degrees C for 0, 4, 8, 16, and 24 h. A fully randomized design was used. There was a decrease in lactic acid (quadratic, p = 0.01), pH, (quadratic, p = 0.02), and yeasts counts (linear, p<0.01) across fermentation times. However, in vitro dry matter disappearance (IVDMD) and ammonia-N increased across fermentation times (quadratic; p<0.01 and p<0.02, respectively). Addition of yeast cells caused a decrease in pH values compared over all fermentation times (p<0.01), and lactic acid decreased at 12 h (p = 0.05). Meanwhile, yeast counts increased (p = 0.01) at 12 h. C. norvegensis increased ammonia-N at 4, 8, 12, and 24 h (p<0.01), and IVDMD of oat straw increased at 8, 12, and 24 h (p<0.01) of fermentation. Yeast cells increased acetate (p<0.01), propionate (p<0.03), and butyrate (p<0.03) at 8 h, while valeriate and isovaleriate increased at 8, 12, and 24 h (p<0.01). The yeast did not affect cellulolytic bacteria (p = 0.05), but cellulolytic fungi increased at 4 and 8 h (p<0.01), whereas production of methane decreased (p<0.01) at 8 h. It is concluded that addition of C. norvegensis to in vitro oat straw fermentation increased ruminal fermentation parameters as well as microbial growth with reduction of methane production. Additionally, yeast inoculum also improved IVDMD. C1 [Ruiz, Oscar; Castillo, Yamicela; Arzola, Claudio; Burrola, Eduviges; Salinas, Jaime; Corral, Agustin; Hume, Michael E.; Murillo, Manuel; Itza, Mateo] Autonomous Univ Chihuahua, Coll Anim Sci & Ecol, Chihuahua 31000, Chih, Mexico. [Castillo, Yamicela; Itza, Mateo] Autonomous Univ Juarez City, Multidisciplinary Div, Dept Vet Med, Nuevo Casas Grandes 31803, Chih, Mexico. [Salinas, Jaime] Autonomous Univ Tamaulipas, Coll Vet Med & Anim Sci, Cd Victoria 87000, Tamps, Mexico. [Hume, Michael E.] ARS, Southern Plains Res Ctr, Food & Feed Safety Res Unit, USDA, College Stn, TX 77843 USA. [Murillo, Manuel] Juarez Univ Durango State, Coll Vet Med & Anim Sci, Durango 34000, Dgo, Mexico. RP Castillo, Y (reprint author), Autonomous Univ Chihuahua, Coll Anim Sci & Ecol, Chihuahua 31000, Chih, Mexico.; Castillo, Y (reprint author), Autonomous Univ Juarez City, Multidisciplinary Div, Dept Vet Med, Nuevo Casas Grandes 31803, Chih, Mexico. EM ycastillo75@yahoo.com NR 48 TC 1 Z9 1 U1 2 U2 4 PU ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC PI SEOUL PA ROOM 708 SAMMO SPOREX, 1638-32, SEOWON-DONG, GWANAK-GU, SEOUL 151-730, SOUTH KOREA SN 1011-2367 EI 1976-5517 J9 ASIAN AUSTRAL J ANIM JI Asian Australas. J. Anim. Sci. PD FEB PY 2016 VL 29 IS 2 BP 211 EP 218 DI 10.5713/ajas.5.0166 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DF6HZ UT WOS:000371457800007 PM 26732446 ER PT J AU Dugger, KM Forsman, ED Franklin, AB Davis, RJ White, GC Schwarz, CJ Burnham, KP Nichols, JD Hines, JE Yackulic, CB Doherty, PF Bailey, L Clark, DA Ackers, SH Andrews, LS Augustine, B Biswell, BL Blakesley, J Carlson, PC Clement, MJ Diller, LV Glenn, EM Green, A Gremel, SA Herter, DR Higley, JM Hobson, J Horn, RB Huyvaert, KP McCafferty, C McDonald, T McDonnell, K Olson, GS Reid, JA Rockweit, J Ruiz, V Saenz, J Sovern, SG AF Dugger, Katie M. Forsman, Eric D. Franklin, Alan B. Davis, Raymond J. White, Gary C. Schwarz, Carl J. Burnham, Kenneth P. Nichols, James D. Hines, James E. Yackulic, Charles B. Doherty, Paul F., Jr. Bailey, Larissa Clark, Darren A. Ackers, Steven H. Andrews, Lawrence S. Augustine, Benjamin Biswell, Brian L. Blakesley, Jennifer Carlson, Peter C. Clement, Matthew J. Diller, Lowell V. Glenn, Elizabeth M. Green, Adam Gremel, Scott A. Herter, Dale R. Higley, J. Mark Hobson, Jeremy Horn, Rob B. Huyvaert, Kathryn P. McCafferty, Christopher McDonald, Trent McDonnell, Kevin Olson, Gail S. Reid, Janice A. Rockweit, Jeremy Ruiz, Viviana Saenz, Jessica Sovern, Stan G. TI The effects of habitat, climate, and Barred Owls on long-term demography of Northern Spotted Owls SO CONDOR LA English DT Article DE Barred Owl; fecundity; Northern Spotted Owl; occupancy; population change; Strix occidentalis caurina; Strix varia; survival ID STRIX OCCIDENTALIS CAURINA; SITE OCCUPANCY DYNAMICS; HOME-RANGE SIZE; VITAL-RATES; SOUTHERN-OSCILLATION; POPULATION-DYNAMICS; CAPTURE-RECAPTURE; NORTHWESTERN CALIFORNIA; SPECIES DISTRIBUTIONS; TERRITORY OCCUPANCY AB Estimates of species' vital rates and an understanding of the factors affecting those parameters over time and space can provide crucial information for management and conservation. We used mark-recapture, reproductive output, and territory occupancy data collected during 1985-2013 to evaluate population processes of Northern Spotted Owls (Strix occidentalis caurina) in 11 study areas in Washington, Oregon, and northern California, USA. We estimated apparent survival, fecundity, recruitment, rate of population change, and local extinction and colonization rates, and investigated relationships between these parameters and the amount of suitable habitat, local and regional variation in meteorological conditions, and competition with Barred Owls (Strix varia). Data were analyzed for each area separately and in a meta-analysis of all areas combined, following a strict protocol for data collection, preparation, and analysis. We used mixed effects linear models for analyses of fecundity, Cormack-Jolly-Seber open population models for analyses of apparent annual survival (phi), and a reparameterization of the Jolly-Seber capture-recapture model (i.e. reverse Jolly-Seber; RJS) to estimate annual rates of population change (lambda(RJS)) and recruitment. We also modeled territory occupancy dynamics of Northern Spotted Owls and Barred Owls in each study area using 2-species occupancy models. Estimated mean annual rates of population change (lambda) suggested that Spotted Owl populations declined from 1.2% to 8.4% per year depending on the study area. The weighted mean estimate of lambda for all study areas was 0.962 (+/- 0.019 SE; 95% CI: 0.925-0.999), indicating an estimated range-wide decline of 3.8% per year from 1985 to 2013. Variation in recruitment rates across the range of the Spotted Owl was best explained by an interaction between total winter precipitation and mean minimum winter temperature. Thus, recruitment rates were highest when both total precipitation (29 cm) and minimum winter temperature (-9.5 degrees C) were lowest. Barred Owl presence was associated with increased local extinction rates of Spotted Owl pairs for all 11 study areas. Habitat covariates were related to extinction rates for Spotted Owl pairs in 8 of 11 study areas, and a greater amount of suitable owl habitat was generally associated with decreased extinction rates. We observed negative effects of Barred Owl presence on colonization rates of Spotted Owl pairs in 5 of 11 study areas. The total amount of suitable Spotted Owl habitat was positively associated with colonization rates in 5 areas, and more habitat disturbance was associated with lower colonization rates in 2 areas. We observed strong declines in derived estimates of occupancy in all study areas. Mean fecundity of females was highest for adults (0.309 +/- 0.027 SE), intermediate for 2-yr-olds (0.179 +/- 0.040 SE), and lowest for 1-yr-olds (0.065 +/- 0.022 SE). The presence of Barred Owls and habitat covariates explained little of the temporal variation in fecundity in most study areas. Climate covariates occurred in competitive fecundity models in 8 of 11 study areas, but support for these relationships was generally weak. The fecundity meta-analysis resulted in 6 competitive models, all of which included the additive effects of geographic region and annual time variation. The 2 top-ranked models also weakly supported the additive negative effects of the amount of suitable core area habitat, Barred Owl presence, and the amount of edge habitat on fecundity. We found strong support for a negative effect of Barred Owl presence on apparent survival of Spotted Owls in 10 of 11 study areas, but found few strong effects of habitat on survival at the study area scale. Climate covariates occurred in top or competitive survival models for 10 of 11 study areas, and in most cases the relationships were as predicted; however, there was little consistency among areas regarding the relative importance of specific climate covariates. In contrast, meta-analysis results suggested that Spotted Owl survival was higher across all study areas when the Pacific Decadal Oscillation (PDO) was in a warming phase and the Southern Oscillation Index (SOI) was negative, with a strongly negative SOI indicative of El Nino events. The best model that included the Barred Owl covariate (BO) was ranked 4th and also included the PDO covariate, but the BO effect was strongly negative. Our results indicated that Northern Spotted Owl populations were declining throughout the range of the subspecies and that annual rates of decline were accelerating in many areas. We observed strong evidence that Barred Owls negatively affected Spotted Owl populations, primarily by decreasing apparent survival and increasing local territory extinction rates. However, the amount of suitable owl habitat, local weather, and regional climatic patterns also were related to survival, occupancy (via colonization rate), recruitment, and, to a lesser extent, fecundity, although there was inconsistency in regard to which covariates were important for particular demographic parameters or across study areas. In the study areas where habitat was an important source of variation for Spotted Owl demographics, vital rates were generally positively associated with a greater amount of suitable owl habitat. However, Barred Owl densities may now be high enough across the range of the Northern Spotted Owl that, despite the continued management and conservation of suitable owl habitat on federal lands, the long-term prognosis for the persistence of Northern Spotted Owls may be in question without additional management intervention. Based on our study, the removal of Barred Owls from the Green Diamond Resources (GDR) study area had rapid, positive effects on Northern Spotted Owl survival and the rate of population change, supporting the hypothesis that, along with habitat conservation and management, Barred Owl removal may be able to slow or reverse Northern Spotted Owl population declines on at least a localized scale. C1 [Dugger, Katie M.] Oregon State Univ, Dept Fisheries & Wildlife, US Geol Survey, Oregon Cooperat Fish & Wildlife Res Unit, Corvallis, OR 97331 USA. [Forsman, Eric D.; Davis, Raymond J.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR USA. [Franklin, Alan B.] USDA, APHIS Natl Wildlife Res Ctr, Ft Collins, CO USA. [White, Gary C.; Burnham, Kenneth P.; Doherty, Paul F., Jr.; Bailey, Larissa; Green, Adam; Huyvaert, Kathryn P.; Ruiz, Viviana] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Schwarz, Carl J.] Simon Fraser Univ, Dept Stat & Actuarial Sci, Burnaby, BC V5A 1S6, Canada. [Nichols, James D.; Hines, James E.; Clement, Matthew J.] US Geol Survey, Patuxent Wildlife Res Ctr, Laurel, MD USA. [Yackulic, Charles B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Clark, Darren A.; Ackers, Steven H.; Andrews, Lawrence S.; McCafferty, Christopher; McDonnell, Kevin; Saenz, Jessica; Sovern, Stan G.] Oregon State Univ, Dept Fisheries & Wildlife, Oregon Cooperat Fish & Wildlife Res Unit, Corvallis, OR 97331 USA. [Augustine, Benjamin] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA USA. [Biswell, Brian L.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Olympia, WA USA. [Blakesley, Jennifer] Rocky Mt Bird Observ, Ft Collins, CO USA. [Carlson, Peter C.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Colorado Cooperat Fish & Wildlife Res Unit, Ft Collins, CO 80523 USA. [Diller, Lowell V.] Green Diamond Resource Co, Korbel, CA USA. [Glenn, Elizabeth M.] US Fish & Wildlife Serv, Portland, OR USA. [Gremel, Scott A.] USDI Natl Pk Serv, Olymp Natl Pk, Port Angeles, WA USA. [Herter, Dale R.] Raedeke Associates, Seattle, WA USA. [Higley, J. Mark] Hoopa Tribal Forestry, Hoopa, CA USA. [Hobson, Jeremy] US Forest Serv, USDA, Pacific Northwest Reg, Springfield, OR USA. [Horn, Rob B.] USDI Bur Land Management, Roseburg Dist Off, Roseburg, OR USA. [McDonald, Trent] West Inc, Laramie, WY USA. [Olson, Gail S.] Washington Dept Fish & Wildlife, Olympia, WA USA. [Reid, Janice A.] US Forest Serv, USDA, Pacific NW Res Stn, Roseburg Field Stn, Roseburg, OR USA. RP Dugger, KM (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, US Geol Survey, Oregon Cooperat Fish & Wildlife Res Unit, Corvallis, OR 97331 USA. EM Katie.dugger@oregonstate.edu FU USDA Forest Service; USDI Bureau of Land Management; USDI National Park Service; Green Diamond Resource Company; Plum Creek Timber Company; Louisiana Pacific Lumber Company; Hancock Forest Management; Hoopa Tribe; USDI Bureau of Land Management via a Cost Reimbursable Research Agreement with Oregon State University FX Funding for demographic studies of Northern Spotted Owls on federal lands was provided primarily by the USDA Forest Service, USDI Bureau of Land Management, and USDI National Park Service. Funding for studies on nonfederal lands came from the Green Diamond Resource Company, Plum Creek Timber Company, Louisiana Pacific Lumber Company, Hancock Forest Management, and the Hoopa Tribe. Funding for the workshop was provided by the USDA Forest Service and USDI Bureau of Land Management via a Cost Reimbursable Research Agreement with Oregon State University. The U.S. Geological Survey and USDA Forest Service required approval of the final manuscript before publication, but none of the funding agencies associated with this meta-analysis had any influence on the content of the submitted or published manuscript. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Ethics statement: All data from study areas in which K.M.D., E.D.F., and A.B.F. were principal investigators (CLE, OLY, COA, HJA, TYE, CAS, and NWC) were collected following protocols approved under animal care and use permits overseen by Oregon State University's and Colorado State University's Institutional Animal Care and Use Committees. These protocols were followed and all relevant state and federal permits were acquired and maintained for all study areas included in this meta-analysis. NR 138 TC 11 Z9 11 U1 29 U2 75 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD FEB PY 2016 VL 118 IS 1 BP 57 EP 116 DI 10.1650/CONDOR-15-24.1 PG 60 WC Ornithology SC Zoology GA DF8LN UT WOS:000371609400006 ER PT J AU Stover, E Inch, S Richardson, ML Hall, DG AF Stover, Ed Inch, Sharon Richardson, Matthew L. Hall, David G. TI Conventional Citrus of Some Scion/Rootstock Combinations Show Field Tolerance under High Huanglongbing Disease Pressure SO HORTSCIENCE LA English DT Article DE citrus breeding; citrus greening; disease resistance; grapefruit; HLB; mandarin hybrid; sweet orange ID DIAPHORINA-CITRI; SWEET ORANGE AB The citrus disease huanglongbing (HLB) has become endemic in Florida, with estimates that greater than 80% of citrus trees are currently infected. Although there are no commercial citrus varieties with strong HLB resistance, some field tolerance has been observed in trees exposed to the disease after they were mature. There is great urgency to identify citrus which may permit 'economic citrus production where HLB is endemic. Therefore, the objective of this study was to assess field tolerance to HLB. To expedite the trial due to urgency, nursery trees were purchased on rootstock varieties as available. The trial included the following unbalanced scion/rootstock combinations: 'Hamlin/Kinkoji', 'Hamlin/Cleopatra', 'Temple/Cleopatra', 'Fallglo/Kinkojr, 'Sugar Belle/Sour Orange', 'Tango/Kuharske', and 'Ruby Red/Kinkoji', with most comparisons based solely on scion/rootstock combinations. A randomized complete block experiment was established at Fort Pierce, FL, in Sept. 2010. All trees exhibited symptoms of HLB and tested positive for the Candidatus Liberibacter asiaticus (CLas) bacterium by Oct. 2012, with similar titers [directly assessed as cycle threshold (Ct) using quantitative polymerase chain reaction (qPCR)] measured for all scion/rootstocks at most sample dates, but early titer development in 'Ruby Red/Kinkoji' was significantly lower than several other scion/rootstocks. Across all time -points, 'Fallglo/Kinkoy had the lowest rating of distinctive HLB mottling and 'Ruby Red/Kinkoji' had the highest rating, but 'SugarBelle/Sour Orange' had the highest percentage of leaves affected. After 5 years, 'SugarBelle/Sour Orange' and 'Tango/Kuharske' had the greatest overall increase in trunk diameter, and were among the healthiest in overall appearance. In Oct. 2015, 'SugarBelle/Sour Orange' and 'Tango/Kuharske' trees had significantly greater fruit load (80-88 fruit/tree) followed by 'Temple/Cleopatra' and 'Fallglo/Kinkojr (31-35 fruit/tree) while 'Hamlin/Kinkoji', 'Hamlin/Cleopatra', and 'Ruby Red/Kinkoji' produced less than 20 fruit per tree. Despite becoming infected by CLas in less than 2 years after planting, the trees continued to grow and all scion/rootstocks displayed increasing fruit production, although very low in 'Hamlin/Kinkoji', 'Hamlin/Cleopatra', and 'Ruby Red/Kinkoji'. Growth and fruit production in the highest performing scion/rootstocks were likely less than would be expected for healthy trees, but these results are promising, with markedly better response of some scion/rootstocks with mandarin hybrid scions compared with trees with sweet orange or grapefruit scions. Larger fully replicated trials are underway. It is noteworthy that the most pronounced HLB symptoms and higher early pathogen titer, which are the two criteria most widely used in assessing HLB resistance, were not associated with the lowest growth and cropping, and focus on early symptomatic traits when screening for resistance may obscure important disease tolerance. C1 [Stover, Ed; Inch, Sharon; Richardson, Matthew L.; Hall, David G.] ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Inch, Sharon] Novozymes North Amer, Franklinton, NC USA. [Richardson, Matthew L.] Univ Dist Columbia, CAUSES, 4200 Connecticut Ave, Washington, DC 20008 USA. RP Stover, E (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM ed.stover@ars.usda.gov FU Citrus Research and Development Foundation FX This work was funded in part by the Citrus Research and Development Foundation. NR 18 TC 5 Z9 5 U1 4 U2 18 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2016 VL 51 IS 2 BP 127 EP 132 PG 6 WC Horticulture SC Agriculture GA DG0JE UT WOS:000371750300002 ER PT J AU Wu, SS Sun, YP Niu, GH Ahland, J Cabrera, R AF Wu, Shasha Sun, Youping Niu, Genhua Ahland, James Cabrera, Raul TI Response of 10 Aster Species to Saline Water Irrigation SO HORTSCIENCE LA English DT Article DE Asteraceae; physiological response; salt tolerance ID SALT TOLERANCE; MECHANISMS; CULTIVARS; NUTRITION; STRESS; GROWTH; PLANTS; CROPS AB Asteraceae is one of the largest plant families with many important garden ornamental species. Salt tolerance of 10 aster perennials was evaluated in a greenhouse experiment, including the following: damianita (Chrysactinia mexicana), gregg's mist flower (Eupatorium greggii), shasta daisy (Leucanthemum xsuperbum 'Becky% blackfoot daisy (Melampodium leucanthum), lavender cotton (Santolina chamaecyparissus), aromatic aster (Symphyotrichum oblongifolium), copper canyon daisy (Tagetes lemmonii), four nerve daisy (Tetraneuris scaposa), skeleton-leaf goldeneye (Viguiera stenoloba), and zexmenia (Wedelia texana). Plants were irrigated with nutrient solution at electrical conductivity (EC) of 1.2 dS.m(-1) (control) or saline solutions at EC of 5.0 or 10.0 dS.m(-1) (EC 5 or EC 10) for 5 weeks. Upon termination, growth parameters, foliar salt damage, relative chlorophyll content [Soil-Plant Analysis Development (SPAD) readings], and mineral concentration were measured. Gregg's mistflower, skeleton-leaf goldeneye, and lavender cotton were the most salt-tolerant species with less reductions in shoot dry weight (DW) in both EC 5 and EC 10. Considering the relatively severe foliar salt damage (visual quality score of 3.1 and 2.7 at EC 5; 2.4 and 1.6 at EC 10) and mortality rate (10% and 40%) in EC 10, aromatic aster and zexmenia should be avoided where poor quality water may be used for irrigation. Gregg's mistflower and skeleton-leaf goldeneye had relatively lower leaf sodium (Na) concentrations suggesting that both species can selectively exclude Na. Damianita and the four daisies, i.e., blackfoot daisy, copper canyon daisy, four-nerve daisy, and shasta daisy, were salt sensitive as evidenced by their greater growth reduction, foliar salt damage, and high Na and chlorine (Cl) accumulation in leaves, and should be avoided in landscapes where poor quality water may be used for irrigation. C1 [Wu, Shasha] Fujian Agr & Forestry Univ, Coll Landscape Architecture, 15 Shangxiadian Rd, Fuzhou 350002, Fujian, Peoples R China. [Wu, Shasha; Sun, Youping; Niu, Genhua] Texas A&M Univ, Texas A&M AgriLife Res Ctr El Paso, 1380 A&M Circle, El Paso, TX 79927 USA. [Ahland, James] USDA ARS, 1680 Madison Ave, Wooster, OH 44691 USA. [Cabrera, Raul] Rutgers State Univ, Rutgers Agr Res & Extens Ctr, 121 Northville Rd, Bridgeton, NJ 08302 USA. RP Sun, YP; Niu, GH (reprint author), Texas A&M Univ, Texas A&M AgriLife Res Ctr El Paso, 1380 A&M Circle, El Paso, TX 79927 USA. EM Youping.sun@ag.tamu.edu; gniu@ag.tamu.edu FU Department of Agriculture (USDA), Agricultural Research Service; USDA National Institute of Food and Agriculture [TEX090450]; Texas A&M AgriLife Research; China Scholarship Council, China FX This research is supported by a Specific Cooperative Agreement from Department of Agriculture (USDA), Agricultural Research Service, the USDA National Institute of Food and Agriculture Hatch project TEX090450, and Texas A&M AgriLife Research.; The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies. Shasha Wu was a visiting scholar at Texas A&M AgriLife Research Center at El Paso financed by China Scholarship Council, China. NR 23 TC 1 Z9 1 U1 4 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2016 VL 51 IS 2 BP 197 EP 201 PG 5 WC Horticulture SC Agriculture GA DG0JE UT WOS:000371750300013 ER PT J AU Bischoff-Ferrari, HA Dawson-Hughes, B Orav, J Staehelin, HB Meyer, OW Theiler, R Dick, W Willett, WC Egli, A AF Bischoff-Ferrari, Heike A. Dawson-Hughes, Bess Orav, John Staehelin, Hannes B. Meyer, Otto W. Theiler, Robert Dick, Walter Willett, Walter C. Egli, Andreas TI Monthly High-Dose Vitamin D Treatment for the Prevention of Functional Decline A Randomized Clinical Trial SO JAMA INTERNAL MEDICINE LA English DT Article ID TANDEM MASS-SPECTROMETRY; SKELETAL-MUSCLE TISSUE; D SUPPLEMENTATION; HIP FRACTURE; CALCIUM SUPPLEMENTATION; 1,25-DIHYDROXYVITAMIN-D3 RECEPTORS; SEQUENTIAL METAANALYSIS; ORAL VITAMIN-D-3; NURSING-HOME; OLDER-ADULTS AB IMPORTANCE Vitamin D deficiency has been associated with poor physical performance. OBJECTIVE To determine the effectiveness of high-dose vitamin D in lowering the risk of functional decline. DESIGN, SETTING, AND PARTICIPANTS One-year, double-blind, randomized clinical trial conducted in Zurich, Switzerland. The screening phase was December 1, 2009, to May 31, 2010, and the last study visit was in May 2011. The dates of our analysis were June 15, 2012, to October 10, 2015. Participants were 200 community-dwelling men and women 70 years and older with a prior fall. INTERVENTIONS Three study groups with monthly treatments, including a low-dose control group receiving 24 000 IU of vitamin D-3 (24 000 IU group), a group receiving 60 000 IU of vitamin D-3 (60 000 IU group), and a group receiving 24 000 IU of vitamin D-3 plus 300 mu g of calcifediol (24 000 IU plus calcifediol group). MAIN OUTCOMES AND MEASURES The primary end point was improving lower extremity function (on the Short Physical Performance Battery) and achieving 25-hydroxyvitamin D levels of at least 30 ng/mL at 6 and 12 months. A secondary end point was monthly reported falls. Analyses were adjusted for age, sex, and body mass index. RESULTS The study cohort comprised 200 participants (men and women >= 70 years with a prior fall). Their mean age was 78 years, 67.0% (134 of 200) were female, and 58.0% (116 of 200) were vitamin D deficient (<20 ng/mL) at baseline. Intent-to-treat analyses showed that, while 60 000 IU and 24 000 IU plus calcifediol were more likely than 24 000 IU to result in 25-hydroxyvitamin D levels of at least 30 ng/mL (P = .001), they were not more effective in improving lower extremity function, which did not differ among the treatment groups (P = .26). However, over the 12-month follow-up, the incidence of falls differed significantly among the treatment groups, with higher incidences in the 60 000 IU group (66.9%; 95% CI, 54.4% to 77.5%) and the 24 000 IU plus calcifediol group (66.1%; 95% CI, 53.5%-76.8%) group compared with the 24 000 IU group (47.9%; 95% CI, 35.8%-60.3%) (P = .048). Consistent with the incidence of falls, the mean number of falls differed marginally by treatment group. The 60 000 IU group (mean, 1.47) and the 24 000 IU plus calcifediol group (mean, 1.24) had higher mean numbers of falls compared with the 24 000 IU group (mean, 0.94) (P = .09). CONCLUSIONS AND RELEVANCE Although higher monthly doses of vitamin D were effective in reaching a threshold of at least 30 ng/mL of 25-hydroxyvitamin D, they had no benefit on lower extremity function and were associated with increased risk of falls compared with 24 000 IU. C1 [Bischoff-Ferrari, Heike A.; Meyer, Otto W.; Egli, Andreas] Univ Zurich Hosp, Dept Geriatr & Aging Res, Raemistr 101, CH-8091 Zurich, Switzerland. [Bischoff-Ferrari, Heike A.; Meyer, Otto W.; Theiler, Robert; Egli, Andreas] Univ Zurich, Ctr Aging & Mobil, Zurich, Switzerland. [Dawson-Hughes, Bess] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Orav, John] Harvard Univ, TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Staehelin, Hannes B.] Univ Basel, Dept Geriatr, Basel, Switzerland. [Dick, Walter] Univ Basel, Dept Orthoped Surg, Basel, Switzerland. [Willett, Walter C.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. RP Bischoff-Ferrari, HA (reprint author), Univ Zurich Hosp, Dept Geriatr & Aging Res, Raemistr 101, CH-8091 Zurich, Switzerland. EM heike.bischoff@usz.ch FU Swiss National Science Foundation [PP00B-114864]; VELUX Foundations; Merck Sharp Dohme AG; WILD; DSM Nutritional Products FX The primary funding sources for this trial were professorship grant PP00B-114864 from the Swiss National Science Foundation and The VELUX Foundations. Supplementary funding was provided by investigator-initiated funds from Merck Sharp & Dohme AG, WILD, and DSM Nutritional Products. NR 56 TC 37 Z9 37 U1 7 U2 11 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6106 EI 2168-6114 J9 JAMA INTERN MED JI JAMA Intern. Med. PD FEB PY 2016 VL 176 IS 2 BP 175 EP 183 DI 10.1001/jamainternmed.2015.7148 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA DD0RR UT WOS:000369627600008 PM 26747333 ER PT J AU Nizalov, D Thornsbury, S Loveridge, S Woods, M Zadorozhna, O AF Nizalov, Denys Thornsbury, Suzanne Loveridge, Scott Woods, Mollie Zadorozhna, Olha TI Security of property rights and transition in land use SO JOURNAL OF COMPARATIVE ECONOMICS LA English DT Article DE Property rights; Agricultural development; Crop mix; Transition economy; Ukraine ID EUROPE AB Price and yield uncertainty are traditional considerations in agricultural markets and their impact on development. Agricultural producers in transition economies face an additional risk factor - changes in the institutional protection of property rights. This paper illustrates how institutional uncertainty may affect investment, land use, and crop mix patterns. In particular, in the Ukrainian example, the rights of tenants are viewed as uncertain in anticipation of establishment of an open market for sale of agricultural land. Establishment of the land market in Ukraine has been postponed several times over the last 15 years and a significant number of lease contracts are not formalized. A large panel of farm-level data was used to show that a higher share of rented land is associated with a lower share of land used for investment intensive perennial crops controlling for prices and other factors. The difference in response to uncertainty is found to be significant among three crop types: perennials, grains and oil crops. The implication is that the lower level of protection of use rights and uncertainty regarding the future regulation of land sales market lead to under-investments in more capital intensive crops. As a result, tenants deviate from the optimal crop mix, reducing the productivity of tenant farms. Farms under 200 ha are affected most negatively as they are less likely to be able to access the level of legal and political protection enjoyed by large farms. Journal of Comparative Economics 44 (1) (2016) 76-91. Kyiv Economics Institute at the Kyiv School of Economics, Ukraine, University of Kent, United Kingdom; USDA Economic Research Service, United States; Michigan State University, United States; Lazarski University, Poland. (C) 2015 Association for Comparative Economic Studies. Published by Elsevier Inc. All rights reserved. C1 [Nizalov, Denys] Kyiv Sch Econ, Kyiv Econ Inst, Kiev, Ukraine. [Nizalov, Denys] Univ Kent, Canterbury CT2 7NZ, Kent, England. [Thornsbury, Suzanne] ERS, USDA, New York, NY USA. [Loveridge, Scott; Woods, Mollie] Michigan State Univ, E Lansing, MI 48824 USA. [Zadorozhna, Olha] Lazarski Univ, Warsaw, Poland. RP Nizalov, D (reprint author), Kyiv Sch Econ, Kyiv Econ Inst, Kiev, Ukraine.; Nizalov, D (reprint author), Univ Kent, Canterbury CT2 7NZ, Kent, England. EM nizalov@kse.org.ua; sthornsbury@ers.usda.gov; loverid2@msu.edu; willi751@msu.edu; o.zadorozhna@lazarski.edu.pl FU NSF [CNH 0909378] FX This research project was partially supported by NSF Award CNH 0909378. The authors greatly appreciate the support from the State Statistics Service of Ukraine for their methodological comments and access to data. The views in this paper do not necessarily represent those of USDA or ERS. NR 22 TC 0 Z9 0 U1 3 U2 12 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-5967 EI 1095-7227 J9 J COMP ECON JI J. Comp. Econ. PD FEB PY 2016 VL 44 IS 1 SI SI BP 76 EP 91 DI 10.1016/j.jce.2015.10.008 PG 16 WC Economics SC Business & Economics GA DG1QZ UT WOS:000371843900005 ER PT J AU Scudiero, E Corwin, DL Wienhold, BJ Bosley, B Shanahan, JF Johnson, CK AF Scudiero, Elia Corwin, Dennis L. Wienhold, Brian J. Bosley, Bruce Shanahan, John F. Johnson, Cinthia K. TI Downscaling Landsat 7 canopy reflectance employing a multi-soil sensor platform SO PRECISION AGRICULTURE LA English DT Article DE Landsat 7; Downscaling; Apparent electrical conductivity; Bare-soil imagery; Micro-elevation; Geographically-weighted regression ID ELECTRICAL-CONDUCTIVITY; SPATIAL VARIABILITY; MANAGEMENT ZONES; GRAIN-YIELD; REMOTE; MODEL; REGRESSION; MAIZE; SALINITY; QUALITY AB Crop growth and yield can be efficiently monitored using canopy reflectance. However, the spatial resolution of freely available remote sensing data is too coarse to fully understand the spatial dynamics of crop status. The objective of this study was to downscale Landsat 7 (L7) reflectance from the native resolution of 30 x 30 m to that typical of yield maps (ca. 5 x 5 m) over two fields in northeastern Colorado, USA. The fields were cultivated with winter wheat (Triticum aestivum L.) in the 2002-2003 growing season. Geospatial yield measurements were available (1 per ca. 20 m(2)). Geophysical (apparent soil electrical conductivity and bare-soil imagery) and terrain (micro-elevation) data were acquired (resolution <5 x 5 m) to characterize soil spatial variability. Geographically-weighted regressions were established to study the relationships between L7 reflectance and the geophysical and terrain data at the 30 x 30 m scale. Geophysical and terrain sensors could describe a large portion of the L7 reflectance spatial variability (0.83 < R-2 < 0.94). Maps for regression parameters and intercept were obtained at 30 x 30 m and used to estimate the L7 reflectance at 5 x 5 m resolution. To independently assess the quality of the downscaling procedure, yield maps were used. In both fields, the 5 x 5 m estimated reflectance showed stronger correlations (average increase in explained variance = 3.2 %) with yield than at the 30 x 30 m resolution. Land resource managers, producers, agriculture consultants, extension specialists and Natural Resource Conservation Service field staff would be the beneficiaries of downscaled L7 reflectance data. C1 [Scudiero, Elia; Corwin, Dennis L.] USDA ARS, US Salin Lab, 450 West Big Springs, Riverside, CA 92507 USA. [Wienhold, Brian J.] Univ Nebraska Lincoln East Campus, USDA ARS, Agroecosyst Management Res Unit, Keim Hall, Lincoln, NE 68583 USA. [Bosley, Bruce] Colorado State Univ Extens, 508 South 10th Ave, Sterling, CO 80751 USA. [Shanahan, John F.] DuPont Pioneer Hibred Int Inc, 6807 Ridge Rd, Lincoln, NE 68512 USA. [Johnson, Cinthia K.] Plainview Farms Inc, 11416 Cty Rd 63, Sterling, CO 80751 USA. RP Scudiero, E (reprint author), USDA ARS, US Salin Lab, 450 West Big Springs, Riverside, CA 92507 USA. EM elia.scudiero@ars.usda.gov RI Scudiero, Elia/K-3580-2013; OI Scudiero, Elia/0000-0003-4944-721X NR 56 TC 1 Z9 1 U1 3 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD FEB PY 2016 VL 17 IS 1 BP 53 EP 73 DI 10.1007/s11119-015-9406-9 PG 21 WC Agriculture, Multidisciplinary SC Agriculture GA DF8WT UT WOS:000371642000004 ER PT J AU Reginster, JY Cooper, C Rizzoli, R Kanis, JA Appelboom, G Bautmans, I Bischoff-Ferrari, HA Boers, M Brandi, ML Bruyere, O Cherubini, A Flamion, B Fielding, RA Gasparik, AI Van Loon, L McCloskey, E Mitlak, BH Pilotto, A Reiter-Niesert, S Rolland, Y Tsouderos, Y Visser, M Cruz-Jentoft, AJ AF Reginster, Jean-Yves Cooper, Cyrus Rizzoli, Rene Kanis, John A. Appelboom, Geoff Bautmans, Ivan Bischoff-Ferrari, Heike A. Boers, Maarten Brandi, Maria Luisa Bruyere, Olivier Cherubini, Antonio Flamion, Bruno Fielding, Roger A. Gasparik, Andrea Ildiko Van Loon, Luc McCloskey, Eugene Mitlak, Bruce H. Pilotto, Alberto Reiter-Niesert, Suzanne Rolland, Yves Tsouderos, Yannis Visser, Marjolein Cruz-Jentoft, Alfonso J. TI Recommendations for the conduct of clinical trials for drugs to treat or prevent sarcopenia SO AGING CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Review DE Clinical trials; Sarcopenia; Public health; Preventative health care; Frailty ID QUALITY-OF-LIFE; PHYSICAL PERFORMANCE BATTERY; LOWER-EXTREMITY FUNCTION; OLDER-ADULTS; MUSCLE STRENGTH; BODY-COMPOSITION; SKELETAL-MUSCLE; GAIT-SPEED; MOBILITY LIMITATIONS; LEAN MASS AB Sarcopenia is an age-related muscle condition which is frequently a precursor of frailty, mobility disability and premature death. It has a high prevalence in older populations and presents a considerable social and economic burden. Potential treatments are under development but, as yet, no guidelines support regulatory studies for new drugs to manage sarcopenia. The objective of this position paper is therefore to suggest a set of potential endpoints and target population definitions to stimulate debate and progress within the medico-scientific and regulatory communities. A multidisciplinary expert working group was hosted by the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis, which reviewed and discussed the recent literature from a perspective of clinical experience and guideline development. Relevant parallels were drawn from the development of definition of osteoporosis as a disease and clinical assessment of pharmaceutical treatments for that indication. A case-finding decision tree is briefly reviewed with a discussion of recent prevalence estimations of different relevant threshold values. The selection criteria for patients in regulatory studies are discussed according to the aims of the investigation (sarcopenia prevention or treatment) and the stage of project development. The possible endpoints of such studies are reviewed and a plea is made for the establishment of a core outcome set to be used in all clinical trials of sarcopenia. The current lack of guidelines for the assessment of new therapeutic treatments for sarcopenia could potentially hinder the delivery of effective medicines to patients at risk. C1 [Reginster, Jean-Yves; Bruyere, Olivier] Univ Liege, Dept Publ Hlth Epidemiol & Hlth Econ, CHU Sart Tilman, B23, B-4000 Liege, Belgium. [Cooper, Cyrus] Univ Southampton, MRC Lifecourse Epidemiol Unit, Southampton, Hants, England. [Cooper, Cyrus] Univ Oxford, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. [Rizzoli, Rene] Univ Hosp Geneva, Serv Bone Dis, Geneva, Switzerland. [Rizzoli, Rene] Fac Med, Geneva, Switzerland. [Kanis, John A.; McCloskey, Eugene] Univ Sheffield, Ctr Metab Bone Dis, Sheffield, S Yorkshire, England. [Kanis, John A.; McCloskey, Eugene] Univ Sheffield, Ctr Integrated Res Musculoskeletal Ageing, Sheffield, S Yorkshire, England. [Appelboom, Geoff] Columbia Univ, Neurol Inst, Med Ctr, New York, NY USA. [Bautmans, Ivan] Vrije Univ Brussel, Gerontol & Frailty Ageing Res Dept, Brussels, Belgium. [Bischoff-Ferrari, Heike A.] Univ Zurich Hosp, Dept Geriatr, CH-8091 Zurich, Switzerland. [Boers, Maarten] Vrije Univ Amsterdam Med Ctr, Dept Epidemiol & Biostat, Amsterdam, Netherlands. [Boers, Maarten] Vrije Univ Amsterdam Med Ctr, Amsterdam Rheumatol & Immunol Ctr, Amsterdam, Netherlands. [Brandi, Maria Luisa] Univ Florence, Dept Internal Med, Florence, Italy. [Cherubini, Antonio] IRCCS INRCA, Geriatr & Geriatr Emergency Care, Ancona, Italy. [Flamion, Bruno] Univ Namur, NARILIS, Res Unit Mol Physiol URPHYM, Namur, Belgium. [Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gasparik, Andrea Ildiko] Univ Med & Pharm Tirgu Mures, Dept Publ Hlth & Hlth Management, Targu Mures, Romania. [Van Loon, Luc] Maastricht Univ, NUTRIM Sch Nutr Toxicol & Metab, NL-6200 MD Maastricht, Netherlands. [Mitlak, Bruce H.] Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA. [Pilotto, Alberto] EO Galliera Hosp, NR HS, Frailty Area, Dept OrthoGeriatr Rehabil & Stabilizat, Genoa, Italy. [Rolland, Yves] Univ Toulouse 3, CHU Purpan, Gerontopole Toulouse, F-31062 Toulouse, France. [Tsouderos, Yannis] Inst Rech Int Servier, Suresnes, France. [Visser, Marjolein] Vrije Univ Amsterdam, Dept Hlth Sci, Amsterdam, Netherlands. [Visser, Marjolein] Vrije Univ Amsterdam Med Ctr, Internal Med, Dept Nutr & Dietet, Amsterdam, Netherlands. [Cruz-Jentoft, Alfonso J.] Hosp Univ Ramon y Cajal, Serv Geriatria, Madrid, Spain. RP Bruyere, O (reprint author), Univ Liege, Dept Publ Hlth Epidemiol & Hlth Econ, CHU Sart Tilman, B23, B-4000 Liege, Belgium. EM olivier.bruyere@ulg.ac.be OI Cherubini, Antonio/0000-0003-0261-9897; Bautmans, Ivan/0000-0002-6820-9586 FU Medical Research Council of UK; US Department of Agriculture [58-1950-0-014] FX Funding was provided by the Medical Research Council of UK (C. Cooper), by the US Department of Agriculture under agreement No. 58-1950-0-014 (R. A. Fielding) (the US Department of Agriculture wishes to note that the opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the Department). NR 86 TC 8 Z9 8 U1 9 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1594-0667 EI 1720-8319 J9 AGING CLIN EXP RES JI Aging Clin. Exp. Res. PD FEB PY 2016 VL 28 IS 1 BP 47 EP 58 DI 10.1007/s40520-015-0517-y PG 12 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA DF3MY UT WOS:000371249700005 PM 26717937 ER PT J AU Evans, KO Compton, DL Laszlo, JA Appell, M AF Evans, Kervin O. Compton, David L. Laszlo, Joseph A. Appell, Michael TI Feruloyl glycerol and 1,3-diferuloyl glycerol antioxidant behavior in phospholipid vesicles SO CHEMISTRY AND PHYSICS OF LIPIDS LA English DT Article DE Feruloyl glycerol; Diferuloyl glycerol; Fluorescence; Vesicles; Antioxidation ID ENZYMATIC-SYNTHESIS; CINNAMOYL GLYCEROL; CANDIDA-ANTARCTICA; FLUORESCENCE; MEMBRANES; LIPASE; ACID; PARTITION; LOCATION; FERULATE AB Feruloyl-sn-glycerol (FG) and 1,3-diferuloyl-sn-glycerol (F(2)G), the by-product of biocatalytic trans-esterification soybean oil and ethyl ferulate, were examined for their behavior in phospholipid vesicles. Based on absorbance and fluorescence methods, FG and F(2)G both were found to partition into vesicles and incorporate well into 1,2-dioleoylphosphocholine (DOPC) vesicles. FG and F(2)G incorporation resulted in vesicles that were as or slightly more stable than the unloaded vesicles. FG and F(2)G both demonstrated the ability to maintain antioxidant properties within the lipid bilayer. Bilayer depth analysis was conducted using the parallax method and molecular modeling. Published by Elsevier Ireland Ltd. C1 [Evans, Kervin O.; Compton, David L.; Laszlo, Joseph A.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat, Res Ctr,USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Appell, Michael] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat, Res Ctr,USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Evans, KO (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat, Res Ctr,USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Kervin.Evans@ars.usda.gov NR 45 TC 1 Z9 1 U1 5 U2 6 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0009-3084 EI 1873-2941 J9 CHEM PHYS LIPIDS JI Chem. Phys. Lipids PD FEB PY 2016 VL 195 BP 1 EP 11 DI 10.1016/j.chemphyslip.2015.11.001 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DF4ZS UT WOS:000371362100001 PM 26561732 ER PT J AU Li, JH Hunt, JF Gong, SQ Cai, ZY AF Li, Jinghao Hunt, John F. Gong, Shaoqin Cai, Zhiyong TI Simplified analytical model and balanced design approach for light-weight wood-based structural panel in bending SO COMPOSITE STRUCTURES LA English DT Article DE Analytical modeling; Failure criterion; Mechanical testing; Balanced design; Bending behavior; Tri-axial core ID SANDWICH PANELS; LAYERWISE THEORY; COMPOSITE GRIDS; PLATES; FORMULATION AB This paper presents a simplified analytical model and balanced design approach for modeling lightweight wood-based structural panels in bending. Because many design parameters are required to input for the model of finite element analysis (FEA) during the preliminary design process and optimization, the equivalent method was developed to analyze the mechanical performance of panels based on experimental results. The bending deflection, normal strain and shear strain of the panels with various configurations were investigated using four point bending test. The results from the analytical model matched well with the experimental data, especially, the prediction for maximum deflection of the panels under failure load. The normal strain and shear strain calculated by the model also agreed with the experimental data. The failure criterion was determined by the failure modes using a 3-dimensional diagram with apparent normal and shear strain. For demonstration, panels 1 and 2 with a fixed core were modeled using the balanced design approach for optimal face thickness. The results showed that both the 3-dimensional diagram and analytical model provided similar thickness results, which were verified by the FEA for wood-based structural panels. Published by Elsevier Ltd. C1 [Li, Jinghao; Gong, Shaoqin] Univ Wisconsin, Dept Biomed Engn, Wisconsin Inst Discovery, Madison, WI 53715 USA. [Li, Jinghao; Gong, Shaoqin] Univ Wisconsin, Dept Biomed Engn, Wisconsin Inst Mat Sci & Engn, Madison, WI 53715 USA. [Li, Jinghao; Hunt, John F.; Cai, Zhiyong] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Hunt, JF; Cai, ZY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jfhunt@fs.fed.us; zcai@fs.fed.us FU USDA; Forest Products Laboratory FX This work is supported by USDA, Forest Products Laboratory and the authors gratefully acknowledge the support of Sara Fishwild, James Bridwell, Marshall Begel, Dave Simpson and Marc Joyal of EMRSL group for the mechanical testing. NR 30 TC 6 Z9 6 U1 4 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0263-8223 EI 1879-1085 J9 COMPOS STRUCT JI Compos. Struct. PD FEB PY 2016 VL 136 BP 16 EP 24 DI 10.1016/j.compstruct.2015.09.045 PG 9 WC Materials Science, Composites SC Materials Science GA DF3XW UT WOS:000371282900003 ER EF